LOCUS       DAZUFB010000001       108926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZUFB010000001 DAZUFB010000000
VERSION     DAZUFB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 108926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.001"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1523
                     /gene="xdhA"
                     /locus_tag="ACHY3M_000001"
     CDS             <1..1523
                     /gene="xdhA"
                     /locus_tag="ACHY3M_000001"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFS4315000.1"
                     /translation="PMAAFLTSKLGGIPVKVSLSREECFLATRTRHAFTIDGQMGVNR
                     DGTLKGYSLDVLSNTGAYASHGHSIASAGGNKVAYLYPRCAYAYSSKTCYTNLPSAGA
                     MRGYGAPQVVFAVESMLDDAATALGIDPVEIRLRNAAREGDANPLTGKRIYSAGLPEC
                     LEKGRKIFEWEKRRAECQNQQGNLRRGVGVACFSYTSNTWPVGVEIAGARLLMNQDGT
                     INVQSGATEIGQGADTVFSQMVAETVGVPVSDVRVISTQDTDVTPFDPGAFASRQSYV
                     AAPALRSAALLLKEKIIAHAAVMLHQSAMNLTLIKGHIVLVERPEEPLMSLKDLAMDA
                     FYHPERGGQLSAESSIKTTTNPPAFGCTFVDLTVDIALCKVTINRILNVHDSGHILNP
                     LLAEGQVHGGMGMGIGWALFEEMIIDAKSGVVRNPNLLDYKMPTMPDLPQLESAFVEI
                     NEPQSAYGHKSLGEPPIIPVAAAIRNAVKMATGVAINTLPLTPKRLYEEFHLAGLI"
     gene            1534..2412
                     /gene="xdhB"
                     /locus_tag="ACHY3M_000002"
     CDS             1534..2412
                     /gene="xdhB"
                     /locus_tag="ACHY3M_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFS4315001.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            2409..2888
                     /gene="xdhC"
                     /locus_tag="ACHY3M_000003"
     CDS             2409..2888
                     /gene="xdhC"
                     /locus_tag="ACHY3M_000003"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFS4315002.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2928..4706)
                     /gene="ygeV"
                     /locus_tag="ACHY3M_000004"
     CDS             complement(2928..4706)
                     /gene="ygeV"
                     /locus_tag="ACHY3M_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4315003.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAFGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            5185..6372
                     /gene="ygeW"
                     /locus_tag="ACHY3M_000005"
     CDS             5185..6372
                     /gene="ygeW"
                     /locus_tag="ACHY3M_000005"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFS4315004.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            6430..7626
                     /gene="dpaL"
                     /locus_tag="ACHY3M_000006"
     CDS             6430..7626
                     /gene="dpaL"
                     /locus_tag="ACHY3M_000006"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFS4315005.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            7684..8895
                     /gene="ygeY"
                     /locus_tag="ACHY3M_000007"
     CDS             7684..8895
                     /gene="ygeY"
                     /locus_tag="ACHY3M_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFS4315006.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            8948..10333
                     /gene="hyuA"
                     /locus_tag="ACHY3M_000008"
     CDS             8948..10333
                     /gene="hyuA"
                     /locus_tag="ACHY3M_000008"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFS4315007.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            10381..11313
                     /gene="arcC"
                     /locus_tag="ACHY3M_000009"
     CDS             10381..11313
                     /gene="arcC"
                     /locus_tag="ACHY3M_000009"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4315008.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(11354..12979)
                     /gene="yqeB"
                     /locus_tag="ACHY3M_000010"
     CDS             complement(11354..12979)
                     /gene="yqeB"
                     /locus_tag="ACHY3M_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFS4315009.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(13027..13797)
                     /gene="yqeC"
                     /locus_tag="ACHY3M_000011"
     CDS             complement(13027..13797)
                     /gene="yqeC"
                     /locus_tag="ACHY3M_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFS4315010.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            13900..14478
                     /gene="mocA"
                     /locus_tag="ACHY3M_000012"
     CDS             13900..14478
                     /gene="mocA"
                     /locus_tag="ACHY3M_000012"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFS4315011.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            14800..17898
                     /gene="ygfK"
                     /locus_tag="ACHY3M_000013"
     CDS             14800..17898
                     /gene="ygfK"
                     /locus_tag="ACHY3M_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFS4315012.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLAYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            17901..19229
                     /gene="ssnA"
                     /locus_tag="ACHY3M_000014"
     CDS             17901..19229
                     /gene="ssnA"
                     /locus_tag="ACHY3M_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFS4315013.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            19280..20059
                     /gene="ygfM"
                     /locus_tag="ACHY3M_000015"
     CDS             19280..20059
                     /gene="ygfM"
                     /locus_tag="ACHY3M_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFS4315014.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            20056..22926
                     /gene="xdhD"
                     /locus_tag="ACHY3M_000016"
     CDS             20056..22926
                     /gene="xdhD"
                     /locus_tag="ACHY3M_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFS4315015.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            23091..24491
                     /gene="xanQ"
                     /locus_tag="ACHY3M_000017"
     CDS             23091..24491
                     /gene="xanQ"
                     /locus_tag="ACHY3M_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFS4315016.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            24509..25825
                     /gene="guaD"
                     /locus_tag="ACHY3M_000018"
     CDS             24509..25825
                     /gene="guaD"
                     /locus_tag="ACHY3M_000018"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFS4315017.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVIMTLGDDRSIYRTYVDGRLVYERN"
     gene            25861..27228
                     /gene="ghxQ"
                     /locus_tag="ACHY3M_000019"
                     /gene_synonym="ygfQ"
     CDS             25861..27228
                     /gene="ghxQ"
                     /locus_tag="ACHY3M_000019"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFS4315018.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(27264..27752)
                     /gene="ygfS"
                     /locus_tag="ACHY3M_000020"
     CDS             complement(27264..27752)
                     /gene="ygfS"
                     /locus_tag="ACHY3M_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS4315019.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(27752..29671)
                     /gene="ygfT"
                     /locus_tag="ACHY3M_000021"
     CDS             complement(27752..29671)
                     /gene="ygfT"
                     /locus_tag="ACHY3M_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFS4315020.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            30107..31555
                     /gene="uacT"
                     /locus_tag="ACHY3M_000022"
     CDS             30107..31555
                     /gene="uacT"
                     /locus_tag="ACHY3M_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFS4315021.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            31557..31682
                     /gene="yqfG"
                     /locus_tag="ACHY3M_000023"
     CDS             31557..31682
                     /gene="yqfG"
                     /locus_tag="ACHY3M_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFS4315022.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(31679..31750)
                     /locus_tag="ACHY3M_000024"
     CDS             complement(31679..31750)
                     /locus_tag="ACHY3M_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315023.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            31805..32353
                     /gene="idi"
                     /locus_tag="ACHY3M_000025"
     CDS             31805..32353
                     /gene="idi"
                     /locus_tag="ACHY3M_000025"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4315024.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(32396..33913)
                     /gene="lysS"
                     /locus_tag="ACHY3M_000026"
     CDS             complement(32396..33913)
                     /gene="lysS"
                     /locus_tag="ACHY3M_000026"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4315025.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33923..35021)
                     /gene="prfB"
                     /locus_tag="ACHY3M_000027"
     CDS             complement(join(33923..34945,34947..35021))
                     /gene="prfB"
                     /locus_tag="ACHY3M_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4315026.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(35112..36845)
                     /gene="recJ"
                     /locus_tag="ACHY3M_000028"
     CDS             complement(35112..36845)
                     /gene="recJ"
                     /locus_tag="ACHY3M_000028"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4315027.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(36851..37561)
                     /gene="dsbC"
                     /locus_tag="ACHY3M_000029"
     CDS             complement(36851..37561)
                     /gene="dsbC"
                     /locus_tag="ACHY3M_000029"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFS4315028.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(37586..38482)
                     /gene="xerD"
                     /locus_tag="ACHY3M_000030"
     CDS             complement(37586..38482)
                     /gene="xerD"
                     /locus_tag="ACHY3M_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4315029.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            38594..39115
                     /gene="fldB"
                     /locus_tag="ACHY3M_000031"
     CDS             38594..39115
                     /gene="fldB"
                     /locus_tag="ACHY3M_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFS4315030.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(39155..39562)
                     /gene="ygfX"
                     /locus_tag="ACHY3M_000032"
     CDS             complement(39155..39562)
                     /gene="ygfX"
                     /locus_tag="ACHY3M_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFS4315031.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39543..39809)
                     /gene="sdhE"
                     /locus_tag="ACHY3M_000033"
     CDS             complement(39543..39809)
                     /gene="sdhE"
                     /locus_tag="ACHY3M_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFS4315032.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            40052..41032
                     /gene="ygfZ"
                     /locus_tag="ACHY3M_000034"
     CDS             40052..41032
                     /gene="ygfZ"
                     /locus_tag="ACHY3M_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFS4315033.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(41228..41887)
                     /gene="yqfA"
                     /locus_tag="ACHY3M_000035"
     CDS             complement(41228..41887)
                     /gene="yqfA"
                     /locus_tag="ACHY3M_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4315034.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(42051..42362)
                     /gene="yqfB"
                     /locus_tag="ACHY3M_000036"
     CDS             complement(42051..42362)
                     /gene="yqfB"
                     /locus_tag="ACHY3M_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFS4315035.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            42407..43840
                     /gene="bglA"
                     /locus_tag="ACHY3M_000037"
     CDS             42407..43840
                     /gene="bglA"
                     /locus_tag="ACHY3M_000037"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFS4315036.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFRYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(43897..44640)
                     /gene="ygfF"
                     /locus_tag="ACHY3M_000038"
     CDS             complement(43897..44640)
                     /gene="ygfF"
                     /locus_tag="ACHY3M_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4315037.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(44901..47774)
                     /gene="gcvP"
                     /locus_tag="ACHY3M_000039"
     CDS             complement(44901..47774)
                     /gene="gcvP"
                     /locus_tag="ACHY3M_000039"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFS4315038.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(47893..48282)
                     /gene="gcvH"
                     /locus_tag="ACHY3M_000040"
     CDS             complement(47893..48282)
                     /gene="gcvH"
                     /locus_tag="ACHY3M_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFS4315039.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(48306..49400)
                     /gene="gcvT"
                     /locus_tag="ACHY3M_000041"
     CDS             complement(48306..49400)
                     /gene="gcvT"
                     /locus_tag="ACHY3M_000041"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFS4315040.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            49691..49774
                     /locus_tag="ACHY3M_000042"
     CDS             49691..49774
                     /locus_tag="ACHY3M_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315041.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(49848..51050)
                     /gene="ubiI"
                     /locus_tag="ACHY3M_000043"
                     /gene_synonym="visC"
     CDS             complement(49848..51050)
                     /gene="ubiI"
                     /locus_tag="ACHY3M_000043"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFS4315042.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(51073..52251)
                     /gene="ubiH"
                     /locus_tag="ACHY3M_000044"
                     /gene_synonym="visB"
     CDS             complement(51073..52251)
                     /gene="ubiH"
                     /locus_tag="ACHY3M_000044"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFS4315043.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(52248..53573)
                     /gene="pepP"
                     /locus_tag="ACHY3M_000045"
     CDS             complement(52248..53573)
                     /gene="pepP"
                     /locus_tag="ACHY3M_000045"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFS4315044.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(53599..54177)
                     /gene="ygfB"
                     /locus_tag="ACHY3M_000046"
     CDS             complement(53599..54177)
                     /gene="ygfB"
                     /locus_tag="ACHY3M_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFS4315045.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            54345..54674
                     /gene="zapA"
                     /locus_tag="ACHY3M_000047"
     CDS             54345..54674
                     /gene="zapA"
                     /locus_tag="ACHY3M_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4315046.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            54716..54899
                     /gene="ssrS"
                     /locus_tag="ACHY3M_000048"
     ncRNA           54716..54899
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHY3M_000048"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            54920..55522
                     /gene="fau"
                     /locus_tag="ACHY3M_000049"
     CDS             54920..55522
                     /gene="fau"
                     /locus_tag="ACHY3M_000049"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4315047.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(55623..55682)
                     /locus_tag="ACHY3M_000050"
     CDS             complement(55623..55682)
                     /locus_tag="ACHY3M_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4315048.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(56015..56074)
                     /gene="ibsC"
                     /locus_tag="ACHY3M_000051"
     CDS             complement(56015..56074)
                     /gene="ibsC"
                     /locus_tag="ACHY3M_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFS4315049.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(56303..57535)
                     /gene="serA"
                     /locus_tag="ACHY3M_000052"
     CDS             complement(56303..57535)
                     /gene="serA"
                     /locus_tag="ACHY3M_000052"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4315050.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(57791..58450)
                     /gene="rpiA"
                     /locus_tag="ACHY3M_000053"
     CDS             complement(57791..58450)
                     /gene="rpiA"
                     /locus_tag="ACHY3M_000053"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4315051.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(58506..58691)
                     /locus_tag="ACHY3M_000054"
     CDS             complement(58506..58691)
                     /locus_tag="ACHY3M_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315052.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            58833..59726
                     /gene="argP"
                     /locus_tag="ACHY3M_000055"
     CDS             58833..59726
                     /gene="argP"
                     /locus_tag="ACHY3M_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFS4315053.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            59930..62074
                     /gene="scpA"
                     /locus_tag="ACHY3M_000056"
     CDS             59930..62074
                     /gene="scpA"
                     /locus_tag="ACHY3M_000056"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFS4315054.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            62067..63062
                     /gene="meaB"
                     /locus_tag="ACHY3M_000057"
     CDS             62067..63062
                     /gene="meaB"
                     /locus_tag="ACHY3M_000057"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFS4315055.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            63073..63858
                     /gene="scpB"
                     /locus_tag="ACHY3M_000058"
     CDS             63073..63858
                     /gene="scpB"
                     /locus_tag="ACHY3M_000058"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFS4315056.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            63882..65360
                     /gene="scpC"
                     /locus_tag="ACHY3M_000059"
     CDS             63882..65360
                     /gene="scpC"
                     /locus_tag="ACHY3M_000059"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFS4315057.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRASYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(65357..66253)
                     /gene="ygfI"
                     /locus_tag="ACHY3M_000060"
     CDS             complement(65357..66253)
                     /gene="ygfI"
                     /locus_tag="ACHY3M_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4315058.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(66420..67160)
                     /gene="yggE"
                     /locus_tag="ACHY3M_000061"
     CDS             complement(66420..67160)
                     /gene="yggE"
                     /locus_tag="ACHY3M_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFS4315059.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(67253..67888)
                     /gene="argO"
                     /locus_tag="ACHY3M_000062"
     CDS             complement(67253..67888)
                     /gene="argO"
                     /locus_tag="ACHY3M_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFS4315060.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(68027..68887)
                     /gene="mscS"
                     /locus_tag="ACHY3M_000063"
     CDS             complement(68027..68887)
                     /gene="mscS"
                     /locus_tag="ACHY3M_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS4315061.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(69246..70325)
                     /gene="fbaA"
                     /locus_tag="ACHY3M_000064"
     CDS             complement(69246..70325)
                     /gene="fbaA"
                     /locus_tag="ACHY3M_000064"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4315062.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(70540..71703)
                     /gene="pgk"
                     /locus_tag="ACHY3M_000065"
     CDS             complement(70540..71703)
                     /gene="pgk"
                     /locus_tag="ACHY3M_000065"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4315063.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(71753..72772)
                     /gene="epd"
                     /locus_tag="ACHY3M_000066"
     CDS             complement(71753..72772)
                     /gene="epd"
                     /locus_tag="ACHY3M_000066"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFS4315064.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            73144..73575
                     /locus_tag="ACHY3M_000067"
     CDS             73144..73575
                     /locus_tag="ACHY3M_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFS4315065.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            73598..74176
                     /locus_tag="ACHY3M_000068"
     CDS             73598..74176
                     /locus_tag="ACHY3M_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4315066.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            74177..74884
                     /locus_tag="ACHY3M_000069"
     CDS             74177..74884
                     /locus_tag="ACHY3M_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS4315067.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRTFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            74872..75549
                     /locus_tag="ACHY3M_000070"
     CDS             74872..75549
                     /locus_tag="ACHY3M_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4315068.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            75543..76220
                     /locus_tag="ACHY3M_000071"
     CDS             75543..76220
                     /locus_tag="ACHY3M_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4315069.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(76192..76905)
                     /locus_tag="ACHY3M_000072"
     CDS             complement(76192..76905)
                     /locus_tag="ACHY3M_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFS4315070.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(76902..77411)
                     /gene="fumE"
                     /locus_tag="ACHY3M_000073"
     CDS             complement(76902..77411)
                     /gene="fumE"
                     /locus_tag="ACHY3M_000073"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFS4315071.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(77433..78398)
                     /gene="glpX"
                     /locus_tag="ACHY3M_000074"
     CDS             complement(77433..78398)
                     /gene="glpX"
                     /locus_tag="ACHY3M_000074"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS4315072.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(78395..79672)
                     /gene="yggP"
                     /locus_tag="ACHY3M_000075"
     CDS             complement(78395..79672)
                     /gene="yggP"
                     /locus_tag="ACHY3M_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS4315073.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(79687..81075)
                     /gene="cmtA"
                     /locus_tag="ACHY3M_000076"
     CDS             complement(79687..81075)
                     /gene="cmtA"
                     /locus_tag="ACHY3M_000076"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS4315074.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(81103..81546)
                     /gene="cmtB"
                     /locus_tag="ACHY3M_000077"
     CDS             complement(81103..81546)
                     /gene="cmtB"
                     /locus_tag="ACHY3M_000077"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFS4315075.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(81860..83851)
                     /gene="tkt"
                     /locus_tag="ACHY3M_000078"
     CDS             complement(81860..83851)
                     /gene="tkt"
                     /locus_tag="ACHY3M_000078"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4315076.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            84129..84887
                     /gene="loiP"
                     /locus_tag="ACHY3M_000079"
                     /gene_synonym="yggG"
     CDS             84129..84887
                     /gene="loiP"
                     /locus_tag="ACHY3M_000079"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFS4315077.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(84943..85686)
                     /locus_tag="ACHY3M_000080"
     CDS             complement(84943..85686)
                     /locus_tag="ACHY3M_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4315078.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(85673..86782)
                     /gene="dgaE"
                     /locus_tag="ACHY3M_000081"
     CDS             complement(85673..86782)
                     /gene="dgaE"
                     /locus_tag="ACHY3M_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFS4315079.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(86786..87646)
                     /locus_tag="ACHY3M_000082"
     CDS             complement(86786..87646)
                     /locus_tag="ACHY3M_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4315080.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(87643..88392)
                     /locus_tag="ACHY3M_000083"
     CDS             complement(87643..88392)
                     /locus_tag="ACHY3M_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4315081.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(88418..88903)
                     /locus_tag="ACHY3M_000084"
     CDS             complement(88418..88903)
                     /locus_tag="ACHY3M_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4315082.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(88914..89342)
                     /locus_tag="ACHY3M_000085"
     CDS             complement(88914..89342)
                     /locus_tag="ACHY3M_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4315083.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(89461..92259)
                     /gene="dagR"
                     /locus_tag="ACHY3M_000086"
     CDS             complement(89461..92259)
                     /gene="dagR"
                     /locus_tag="ACHY3M_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFS4315084.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(92518..93438)
                     /gene="speB"
                     /locus_tag="ACHY3M_000087"
     CDS             complement(92518..93438)
                     /gene="speB"
                     /locus_tag="ACHY3M_000087"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFS4315085.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(93574..94305)
                     /locus_tag="ACHY3M_000088"
     CDS             complement(93574..94305)
                     /locus_tag="ACHY3M_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315086.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(94451..96427)
                     /gene="speA"
                     /locus_tag="ACHY3M_000089"
     CDS             complement(94451..96427)
                     /gene="speA"
                     /locus_tag="ACHY3M_000089"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFS4315087.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQNMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(96436..96567)
                     /gene="yqgB"
                     /locus_tag="ACHY3M_000090"
     CDS             complement(96436..96567)
                     /gene="yqgB"
                     /locus_tag="ACHY3M_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFS4315088.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(96661..96804)
                     /gene="yqgG"
                     /locus_tag="ACHY3M_000091"
     CDS             complement(96661..96804)
                     /gene="yqgG"
                     /locus_tag="ACHY3M_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFS4315089.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(96915..97166)
                     /gene="yqgD"
                     /locus_tag="ACHY3M_000092"
     CDS             complement(96915..97166)
                     /gene="yqgD"
                     /locus_tag="ACHY3M_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFS4315090.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            97222..98376
                     /gene="metK"
                     /locus_tag="ACHY3M_000093"
     CDS             97222..98376
                     /gene="metK"
                     /locus_tag="ACHY3M_000093"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4315091.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            98812..100206
                     /gene="galP"
                     /locus_tag="ACHY3M_000094"
     CDS             98812..100206
                     /gene="galP"
                     /locus_tag="ACHY3M_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFS4315092.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            100283..100780
                     /gene="yggI"
                     /locus_tag="ACHY3M_000095"
     CDS             100283..100780
                     /gene="yggI"
                     /locus_tag="ACHY3M_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFS4315093.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            100875..101582
                     /gene="endA"
                     /locus_tag="ACHY3M_000096"
     CDS             100875..101582
                     /gene="endA"
                     /locus_tag="ACHY3M_000096"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFS4315094.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            101662..102393
                     /gene="rsmE"
                     /locus_tag="ACHY3M_000097"
     CDS             101662..102393
                     /gene="rsmE"
                     /locus_tag="ACHY3M_000097"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4315095.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            102406..103356
                     /gene="gshB"
                     /locus_tag="ACHY3M_000098"
     CDS             102406..103356
                     /gene="gshB"
                     /locus_tag="ACHY3M_000098"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFS4315096.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            103465..104028
                     /gene="yqgE"
                     /locus_tag="ACHY3M_000099"
     CDS             103465..104028
                     /gene="yqgE"
                     /locus_tag="ACHY3M_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFS4315097.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            104028..104444
                     /gene="ruvX"
                     /locus_tag="ACHY3M_000100"
     CDS             104028..104444
                     /gene="ruvX"
                     /locus_tag="ACHY3M_000100"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4315098.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(104620..105600)
                     /gene="yggR"
                     /locus_tag="ACHY3M_000101"
     CDS             complement(104620..105600)
                     /gene="yggR"
                     /locus_tag="ACHY3M_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFS4315099.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            105618..106322
                     /gene="yggS"
                     /locus_tag="ACHY3M_000102"
     CDS             105618..106322
                     /gene="yggS"
                     /locus_tag="ACHY3M_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFS4315100.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            106340..106906
                     /gene="yggT"
                     /locus_tag="ACHY3M_000103"
     CDS             106340..106906
                     /gene="yggT"
                     /locus_tag="ACHY3M_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFS4315101.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            106903..107193
                     /gene="yggU"
                     /locus_tag="ACHY3M_000104"
     CDS             106903..107193
                     /gene="yggU"
                     /locus_tag="ACHY3M_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFS4315102.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            107201..107794
                     /gene="rdgB"
                     /locus_tag="ACHY3M_000105"
     CDS             107201..107794
                     /gene="rdgB"
                     /locus_tag="ACHY3M_000105"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFS4315103.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            107787..108923
                     /gene="hemW"
                     /locus_tag="ACHY3M_000106"
     CDS             107787..108923
                     /gene="hemW"
                     /locus_tag="ACHY3M_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4315104.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT        26487 a        29064 c        27298 g        26077 t
ORIGIN      
        1 agccaatggc ggcattcctg accagcaagc ttggcggcat tccggtgaaa gtttccctta
       61 gccgtgaaga gtgtttcctc gcaacccgta cccgccacgc ttttaccatt gacgggcaaa
      121 tgggcgtgaa ccgcgacgga acattgaaag gttatagtct ggatgttctg tctaacaccg
      181 gcgcttatgc atctcacggg cactccatcg cttctgcggg ggggaataaa gtcgcttacc
      241 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
      301 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgccgttgag tctatgcttg
      361 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
      421 gcgaaggaga tgctaatccg ctcacgggca aacgtattta cagcgcaggg ttgccggagt
      481 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
      541 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
      601 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
      661 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
      721 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaact caagataccg
      781 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
      841 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
      901 tacatcagtc agcgatgaat ctgaccctga taaaaggcca tatcgtgctg gttgaacgac
      961 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1021 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1081 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1141 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1201 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1261 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     1321 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     1381 agtcactggg tgagccacca ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     1441 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     1501 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     1561 gcagcaaccc ttgccgatgc cataaacctg ctggctgaca acccgcaggc caaactgctc
     1621 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     1681 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctcgcta
     1741 cgtatcggct ctgcaacgac atttacccag ctaatagaag atcctataac tcaacgtcat
     1801 ctcccggcgt tatgtgctgc ggccacgtcc attgctggac cgcagatccg taacgtcgct
     1861 acctacggtg gaaatatttg caacggtgcc accagcgcag attctgccac gccaacgcta
     1921 atttatgacg cgaaactgga gatccactcc ccgcgcggtg ttcgtttcgt cccgattaat
     1981 ggctttcaca ccgggccggg caaagtgtct cttgagcatg acgaaatcct cgtcgccttt
     2041 cattttccgc cacagccgaa agaacacgcg ggcagcgcgc attttaaata tgccatgcgc
     2101 gacgcaatgg atatttcaac gattggctgc gccgcacatt gccgactgga taacggcaat
     2161 ttcagcgaat tacgcctggc atttggtgtt gccgcgccaa cgccgattcg ctgccaacat
     2221 gccgaacaga ctgcacaaaa tgcgccatta aacctgcaaa cgctggaagc tatcagcgaa
     2281 tctgtcctgc aagatgtcgc cccgcgttct tcatggcggg ccagtaaaga gtttcgtctg
     2341 catctcatcc agacgatgac caaaaaagtg attagcgaag ccgtcgccgc ggcgggggga
     2401 aaattgcaat gaatcacagc gaaacaatta ccatcgaatg caccattaac gggatgcctt
     2461 ttcagcttca cgccgcacca ggcacgccgc tctcggaatt actccgcgaa caaggactgc
     2521 taagtgtcaa acaagggtgc tgcgtgggtg aatgtggtgc ctgtacggtg ttggtcgacg
     2581 gcacagcaat agacagttgc ttataccttg ccgcctgggc tgaaggaaaa gagatccgca
     2641 cgctggaagg tgaagcgaaa ggcggaaaac tttctcatgt tcagcaggct tatgcgaaat
     2701 ccggcgcagt gcagtgcggg ttttgtacgc ctggcctgat tatggctacc acggcaatgc
     2761 tggcgaaacc acgcgagaag ccattaacca ttacggaaat tcgtcgcgga ctggcgggaa
     2821 atctttgtcg ctgcacgggg tatcagatga ttgtaaatac agttctggat tgcgagaaaa
     2881 cgaagtaaaa ggatatccgg cctgaattca ggccggattc actgaggtta tgtgtttaac
     2941 aactcatatt tcttaatctt gcgatagagc gtagcaatgc cgatgcccag ttcatcagca
     3001 acttgcttct tgctgttatg acgtgaaagc gcctcgcgga tcatttgctt ttccatctcc
     3061 tccagcgccg tgccgcccgc atcatcgagt gacaggtgcg cctcactgac ctctgttaca
     3121 tcactttgct ccgttgtgcc attattcagc agatttggcg gcaatagcgt gctgtcgata
     3181 acttcacctg aaggaactac gttaaccaga tattccatca aattacttaa ctcgcgcagg
     3241 tttccgggcc aacgatgctt acgcaatatt tcgacgacat cgggagcaat gccaggataa
     3301 accgatccca gacgacgggt atgcagatgt aaaaagtaat gcaccaatag ttcaatatct
     3361 tcctgacgtt cacgcagcgg tggcagagtt atcgggataa cattaagtcg gtagaagaga
     3421 tcttcgcgga atttaccttc ggcaatgaac tgggccaaat tctgattagt tgcagaaatg
     3481 atgcgaatgt cgacttgtat tgggctactg gcaccaatcg gtagaatttc acgcgcctca
     3541 atagcgcgca gtaatttagc ctgcaacatt aatggcatat cacctatttc atcgagaaac
     3601 agcgtgcccg tattcgccgc ctgaatcaac cctgttttac cgttggcaga agcgccagta
     3661 aatgcgcctt taacataacc gaacagttcg ctttccagaa gctgctccgg aatcgcggca
     3721 cagttgatag caataaaggg tttattccgt cttccgctca acttatggat tgctcgggcg
     3781 acgacttctt tacccgtgcc gctttcacca accaccataa cgctggatgg gctgggtgca
     3841 atacggctaa tgagtcgttt taattgccgc ataacacggc actcgccaac caattgttca
     3901 atatgtggtt catcaggtgc atttgctaca gaaaaactgg tatgcgattg gtgaaatgcc
     3961 attaaaaata attgtcggcc ctgaatgtta tgcaattgac caatgattaa ttcactttta
     4021 tcgtcccacg aaacaatatg ctgcatatgt ccatgagtaa aattactctc aaatgttaat
     4081 ggtctgaaac ggataggttt cccaataata ttattttgta caacaccaag tgtttttaag
     4141 gcagtctgat taacaaactg aacccgactt tcatcatcaa caactaatac gccctgatcc
     4201 atattatcga tcatggtcgc gaatatttta ctgatgttat ctcctggccc ctgatcctcc
     4261 agaagtttcg aaacaaaaat ggtggatata tggcgaacat aatcagaaaa ttcgcgtaaa
     4321 ttatcactga tatgctcttg ttgctcgtgg gtaacggcaa tcaaacttat caccccaaca
     4381 caacgatcct gtaaaatgac aggcgtaccc agaaatgctt tttcacggca attttcttta
     4441 ctatcgcaac cttcgcaaag gggatcgaag cgagactgtg tcacaacttt ttcagttttc
     4501 gtttccagga cgtggcggag caggcgtgag ttgccgctca actggcgacc aagaaacttc
     4561 ccaaacgcgc ccgttccggc gacgcgacac aagttttcat caacgatctc aacctcaagc
     4621 tgcaaaacgc tggcaagcat tctggcaaaa cgctgaattg tcggttgaat ttgcatcaat
     4681 actgactgcg tagtagcaag ctccatagct ttaccttcca gacttactta aaagtcgatc
     4741 attgaagacg ttgatggttc acagatcatg atgatattaa ctcaggcgaa attggctttg
     4801 ataaaaacat aagattttta tcattttcta atgaaattat ggaagagata tcacatttct
     4861 atatcaatat gagaattacg gcggtgagtt tatcaaactg aagagagata gcctgtccct
     4921 ttatcttatt tctgatactt agcagcaaat aaataacgcg ataaaaaaag ccaaacgttt
     4981 tcgtatttta caaacaaccg gaagctggca tcaatttgtg atcaacccca cacattatcc
     5041 gtcaaattag tcttttgcag ccccgtggat aattctggca cacttattgt tagtcccagg
     5101 tatagctgtg aaaacaccaa tcactttggc aagtcacagt gaaataaacc actttgcctg
     5161 tcattccact accgggactt tatgatgaaa actgttaatg agctgattaa ggatatcaat
     5221 tcgctgacct ctcaccttca cgagaaagat tttttgttaa cgtgggaaca gacgccagat
     5281 gaactgaaac aagtactgga cgttgccgca gcattaaaag cactgcgtgc tgaaaacatc
     5341 tcaaccaaag tctttaatag tggattaggt atttccgtat tccgcgacaa ctccacccgt
     5401 acccgcttct cttatgcttc cgcgcttaac ctgctcggcc ttgcacaaca agatctcgac
     5461 gaaggcaaat cacaaatcgc tcacggcgaa accgtgcgtg aaaccgccaa tatgatctcc
     5521 ttctgcgccg acgctattgg tattcgcgac gatatgtatc tgggcgcagg caacgcctat
     5581 atgcgtgaag ttggcgctgc acttgatgac ggttacaagc agggtgtact gccacagcgt
     5641 ccggctttag tgaacctgca atgcgatatt gaccacccga ctcagtcaat ggctgacctc
     5701 gcgtggttac gtgaacactt tggttcactg gaaaacctga aaggtaaaaa aatcgccatg
     5761 acctgggcct actctccaag ctatggcaaa ccgctctctg taccacaagg catcatcggt
     5821 ctgatgactc gcttcggtat ggatgtcacc ctggcccatc cggaaggcta cgacctgatc
     5881 ccggatgtgg ttgaagtggc gaaaaacaat gctaaagcat ctggcggtag cttccgtcag
     5941 gtcaccagca tggaagaagc gttcaaagat gcagacatcg tttatccgaa gtcatgggca
     6001 ccttacaaag tgatggaaca gcgtaccgaa ttgctgcgcg ctaacgatca cgaaggctta
     6061 aaagcactgg aaaaacagtg tctggcacag aacgcgcaac acaaagactg gcattgtact
     6121 gaagagatga tggaactgac ccgtgatggc gaagccctgt acatgcactg cctgccagct
     6181 gatatcagcg gcgtatcctg caaagaaggg gaagtgactg aaggcgtatt cgaaaaatac
     6241 cgtatcgcta cctacaaaga agccagctgg aagccttata tcatcgccgc gatgatcctg
     6301 tcccgtaaat acgccaaacc aggtgcactg ctcgagcaac tgctgaaaga agcgcaagaa
     6361 cgcgtgaaat aacccctcgg gccgatcagc tggtcggccc tctatttcca gaggccaaaa
     6421 ggataggata tgtccgtttt ctcattgaag attgatatcg ccgataacaa atttttcaac
     6481 ggcgaaacat caccgctctt ttcgcaaagc caggccaaac tggcgcgcca gttccaccag
     6541 aaaatagctg gttatcgccc aacaccgctt tgtgcgctgg acgatctcgc aaaccttttt
     6601 ggggtgaaga aaattcttgt caaagacgaa tcaaaacgat tcggtctgaa cgccttcaaa
     6661 atgctcggcg gtgcgtacgc tatcgctcaa ttattgtgtg aaaaatatca tcttgatatc
     6721 gaaacgctgt catttgagca cctgaaaaat gccatcggcg aaaaaatgac tttcgcgacc
     6781 accaccgacg gcaaccacgg gcgcggtgtg gcgtgggcag cgcagcaact cggacagaat
     6841 gcggtgattt acatgccgaa aggttctgct caggaacgcg ttgacgccat tctgaacctc
     6901 ggtgccgagt gcatcgtcac agatatgaac tatgacgata ccgttcgcct gaccatgcaa
     6961 cacgcgcagc agcacggctg ggaagtggta caggacacgg catgggaagg ttacaccaaa
     7021 atcccaacct ggatcatgca aggctacgca accctggcag atgaagccgt cgagcaaatg
     7081 cgtgaaatgg gcgtaacccc gacgcacgtt ctgctgcaag ccggtgtcgg agcaatggcc
     7141 ggtggtgtgc tgggttatct ggtcgacgtc tatagcccgc aaaatctgca cagcattatt
     7201 gttgaacctg acaaagctga ctgtatttat cgctccggcg tcaaaggcga catcgtcaac
     7261 gttggcggtg atatggccac catcatggca ggcctggcct gtggcgaacc taacccgctg
     7321 ggctgggaaa tcctacgtaa ctgcgccacc caattcatct cctgccagga cagcgttgcc
     7381 gcattaggta tgcgcgtgct gggtaatccg tacggcaacg acccgcgcat catctccggt
     7441 gaatccggcg ctgtcggttt gggcgttctc gcagcggttc attatcaccc gcaacgtcaa
     7501 agcctgatgg aaaaactggc gctgaacaaa gatgccgtgg tgctggttat cagcactgaa
     7561 ggcgacaccg acgtgaagca ctaccgcgaa gttgtctggg aaggcaaaca cgctgtagca
     7621 ccttaatcac tctattggaa ctactccctg caaaacgggg agtaaaaaat ctggagaaaa
     7681 ataatggcta agaatattcc attcaaactg attcttgaaa aagcaaaaga ttaccaggcg
     7741 gacatgactc gcttcctgcg cgacatggtt gctattccca gtgaaagctg cgacgaaaaa
     7801 cgcgtagtac atcgtattaa agaagagatg gaaaaagtcg gcttcgataa agttgaaatc
     7861 gacccgatgg gcaacgttct cggttatatc ggccacggcc cgcgtctggt ggcaatggac
     7921 gctcatatcg ataccgtcgg cattggcaac atcaaaaact gggacttcga tccgtacgaa
     7981 ggcatggaaa ctgatgagct aatcggtggt cgcggtactt ccgaccagga aggcggcatg
     8041 gcatctatgg tttatgccgg taaaatcatt aaagacctcg gtctggaaga tgaatatacc
     8101 ctgctggtta ccggtactgt gcaggaagaa gactgcgacg gtctgtgctg gcagtacatt
     8161 attgaacaat ccggcattcg cccggaattt gtggtcagta ccgaaccaac cgactgccag
     8221 atataccgtg gtcagcgcgg tcgtatggaa attcgtattg atgttcaggg tgttagctgc
     8281 cacggttctg cgccagaacg cggtgacaac gccattttca aaatgggtcc gattcttggc
     8341 gaattacaag aactctccca acgtctgggt tatgacgaat tcctcggcaa aggcaccctc
     8401 accgtttctg aaatcttctt cacatcccca agccgttgcg ctgtagcaga tagctgcgcc
     8461 gtctctattg accgccgtct gacctggggc gaaacctggg aaggcgcgct ggacgaaatc
     8521 cgcgccctgc ctgcagtaca gaaagctaac gcggttgttt ctatgtacaa ctacgaccgt
     8581 ccgtcctgga ctggcctggt ttacccaacc gaatgctact tcccgacctg gaaagtggaa
     8641 gaagatcact tcaccgttaa agcactggtg aatgcctacg aagggctgtt tggcaaagcg
     8701 ccggttgttg ataagtggac cttctcaact aacggcgtat ctatcatggg ccgtcacggc
     8761 attccggtga tcggctttgg cccgggtaaa gaacctgaag cgcatgcacc taacgaaaaa
     8821 acctggaaat ctcacctggt gacctgtgcc gcgatgtacg ctgcaatccc gttaagctgg
     8881 ctggcaacag agtaattact cttcacttat tcccctccgg ttcgccggag ggtttttgga
     8941 gtttgctatg cgcgtattga tcaaaaacgg cactgtcgtt aacgcagatg gacaagccaa
     9001 acaggatttg ctgattgaaa gcgggattgt tcgccagttg ggcaacaata tttcgccgca
     9061 gctcccgtat gaagaaattg atgccactgg ctgttacgtt ttccctggcg gcgtggatgt
     9121 ccatacgcat ttcaatattg atgtcggcat cgcgcgcagt tgtgatgatt tttttaccgg
     9181 tacccgcgca gctgcgtgtg gcggtacaac aaccattatt gaccatatgg gatttggccc
     9241 aaacggctgt cggttacgcc atcaactgga ggtttatcgt ggttatgccg cccataaagc
     9301 ggtcatcgat tacagctttc acggtgtgat ccagcacatt aatcacgcaa tcctcgacga
     9361 aatcccgatg atggtcgagg aaggactgag cagttttaaa ctctatttaa cctatcaata
     9421 caaactcaac gatgacgagg ttttgcaggc attacgccgt ctgcatgaat ccggcgcgct
     9481 gaccaccgtg cacccggaaa atgatgcggc tatcgccagc aagcgggcgg aatttatcgc
     9541 cgcagggtta accgcgccgc gctatcatgc cttgagtcgc cctctggaat gcgaagcgga
     9601 agccatcgcc cgcatgatta acctggcaca aattgccggt aacgccccgc tctatatcgt
     9661 gcacctgtct aacggcttag gtctggatta tctgcgtctt gcccgtgcga atcaccagcc
     9721 agtctgggtt gaaacctgcc cacaatatct cctgttggac gaacgcagtt acgatacaga
     9781 agacggcatg aagttcattc ttagcccacc gctgcgtaac gtacgcgagc aggacaaact
     9841 gtggtgtggc atcagcgatg gtgcgattga cgtggtggcg accgatcact gcaccttctc
     9901 gatggctcaa cgcctgcaaa tttctaaagg cgatttcagt cgctgcccaa atggcttacc
     9961 cggtgtggaa aaccgcatgc agttactgtt ttccagtggc gtgatgacgg gacgtataac
    10021 accggaacgc tttgttgaat taaccagcgc aatgcccgcc aggctgtttg gcctgtggcc
    10081 gcaaaaagga ttattagcgc ccggttccga tggcgacgtg gtgattatcg acccacgtca
    10141 gagccaacaa attcagcatc gccatctcca cgacaacgcc gactactcgc catgggaggg
    10201 ttttacctgt cagggcgcga ttgtcagaac cttatcccgt ggtgaaacga ttttctgtga
    10261 cggcaccttt acaggcaaag ccgggcgagg tcgtttcctg cgacgcaaac cgtttgtccc
    10321 tcccgtgctc taaccactgt cagggatcta tacacaatca agaatgatga gtgaggaaaa
    10381 atgagtaaga aaattgttct cgccctgggc gggaatgcgc tgggcgatga cctggccgga
    10441 caaatgaaag cggtaaaaat tacttctcag gcaattgttg atttaattgc ccaaggacat
    10501 gaagttatcg tcactcatgg taacgggccg caggtgggca tgattaacca ggcctttgaa
    10561 gctgctgcaa aaactgaagc gcactcgcca atgctgccga tgtctgtctg cgttgcctta
    10621 agccagggtt atattggtta cgatctacaa aacgccttaa gagaagaact gctttctcgg
    10681 ggcattaata aaccggtagc aacgctggtt actcaggtcg aagtcgacgc taacgatccg
    10741 gcattcctca acccgaccaa gccgatcggt tcgttcttta ccgagcaaga agcagagcaa
    10801 ctgacaaaac agggttacac tctgaaagaa gatgctggtc gcggctatcg ccgtgttgtt
    10861 gcctcgccaa aaccggttga tattattgaa aaagaaacgg ttaaagctct ggtagatgca
    10921 ggccaggtgg tgatcaccgt tggcggtggt ggtattcctg ttattcgtga aggtaaccat
    10981 ctgcgtggtg ccagcgcggt tatcgataaa gactgggcca gcgcccgttt agcagaaatg
    11041 attgatgccg atatgctgat cattctgact gcggtagaaa aagtggccat taacttcggt
    11101 aaagagaatg aacagtggct cgatcgcctg tcattaagtg atgctgaacg ctttatcgaa
    11161 gaaggtcatt ttgcgaaagg ttctatgctg cctaaagtgg aagctgccgc ctcgtttgct
    11221 cgctctcgcg caggtcgtga agcactgatt accgtattga gcaaagcgaa agaggggatt
    11281 gaaggcaaaa ccggaacggt gatttgtcag taattttaag gccgggtggg taataccatc
    11341 cggccatttt catttaagcc acttccagca cttcttttgt cgctttcacg ccctgatgca
    11401 tcaacatcat taacgcctca agtacgccgc cgccaatcgc ccgggcttta tcagaaacgc
    11461 tggtgaaatc agccgtttca ccacgaggat cgatatcacc aattttaaaa ccaccgacca
    11521 ctgccaggcc gtcgttcaac aagccacgca ccatccccgt caacggtgct ttaatttcat
    11581 gctcaccaat ccaggcaatc acatcgccct ctttcaccag atcgcctaat ttcacgttgg
    11641 atcgcataat gcctgcagca ggagcacgga tcacccgtcg ggtggtatgc cccataatat
    11701 tgccaggaac accggtattc tcctgcgcac aaccagagta aatcacctga ccgagccagt
    11761 gcccgcgatt tgtttcaatt accgcatgac aatccttccc tgcagtaaag cccggcccaa
    11821 gcgcgattgt tactggtgcc atatctgccc gcgttcccaa attctgtttc gccagaatag
    11881 cgtccaccac gcaaagcggt ttcagttcat caagcaatga acaggcggga tctgccatca
    11941 cagggatgaa tccgcgttcg gtaagtttca tcgcttccgc agagctggtt gccaggcgag
    12001 cagtaacgcc ttcgaccgtc atttcgccat cgaacacggc ctgggcaaac gccacggtac
    12061 aacgaatcac tgtcggtttt tccacttcca gcatgatcac tttaaaaccc gcatgataca
    12121 gacgtagcgc cacaccactg gcgatatctc ccgcaccgcg aatcaccacc agctggtgtc
    12181 cggaaggatg agaaggtttc atcatcagcc cacccggcgc gttatttttc acctgtaata
    12241 tttctgccag cacgctgatg gcgatctcct gcggcgtttc cgcacctatg ttgtaaccaa
    12301 cgggcgcatg taaacgggca atatgttctt cagccacgcc tttctcacgc aattgacgca
    12361 ggaaaagctg aaccttgcgg cgacttgcca gcaaccccaa ccatgcaatg ggctgttcaa
    12421 tgagtttgtc gagggcttca cgatcctgat tattcgtggc aatcaggaca aaattatcag
    12481 ggcgaatatc cagtgcttcc actgctgcac caaacgactc agcatggaga agcgtggttg
    12541 atggagggaa tagttcagga ttgaggcttt cgcgataaat atcggcaacg gcaatatcaa
    12601 atcctaatag cgccgcactc tgggctatcg cccggttgac atgccccgcg ccaatcaaca
    12661 ccagacgtgg acgcatacca tggacgctga taaatactga cattgcacct ccacaatctg
    12721 acccgacagc atccgcaccg ttacgagcca tacgtccatg gaataatcgc ggcttacgtt
    12781 cctgcaatgc ctgaagtgac tcttcaatca ccttccgctc aaccattccg ccaccaattg
    12841 taccgacgat agaaccatcg gcgcgcacta acatttgtgc agaatggcgg ggagtcgagc
    12901 ctcggctatc aacaatttgc gccatcgcaa acggacaatt ttgctcttcg agttttgcag
    12961 cctctgtgaa aatattcata ccaacctcaa tggatatcct ttcagtaacc cggaataccc
    13021 gggccgctaa ttcacaaatc tgcgcgcgat tgcaggatgt tcttgtatat cgcccagcca
    13081 gattgcctct acgtcgtgtt gttgcagcgg ttgaaggagc tcgctttgtg caatcgcatt
    13141 ctcacattga gaaaaacggt taatgaacca gactcgccga caaccttgcg gtacgttttt
    13201 aaacgccccc tgagggtggc gaaccagcgc aacgagatcg ctcagttgca aggttgcatc
    13261 aggtgttaac ccggtaatat cagcaaactg cgaccagcga tggacatttt ctgtgctcac
    13321 tttcgcgccc aaagtatgtc ctcccatcac agcaatcacg cagcaactgc ttttgggtat
    13381 gcaaggttcg tgctcatcag gcgcttttaa cggcattcct cgcgagccat ctgcttcaat
    13441 gagaattacg tcacactctg gtcgttgcac cagtgcatca atcgcttctg gcgtaaatcc
    13501 ctgcactttt ccctggttcg ctttccagct gtgaaaacaa aatgaaatgg gggatgtaag
    13561 agacgcatga ggaagcatgg cgggatcacg acagaaaacc acgggccagt gagacgttgg
    13621 cataaacata tgtgtcgtcg tcgtgattaa cacacgcctg ccgctagcct ggagcagttc
    13681 tgccagccaa aaaagcaagc tggttttccc cccagcacca acaacagaaa tcacagtggg
    13741 acgtttctgc gcacctaaat caatgactaa tgccgatggg tcaactatac ttttcacgag
    13801 tctttatgac ctctctggga taaattatcc ccaacttaat ccatcaggaa gtaacgcaat
    13861 tatcaggcgt tattagcccc tataaataat ggaaccacta tgtcagccat cgactgtata
    13921 attaccgctg ccggattatc atcaagaatg gggcaatgga aaatgatgtt accctggcaa
    13981 caggggacaa ttcttgatac aagtatcaaa aatgcgttgc agttttgtag ccgaattatt
    14041 ttagtcaccg gctatcgtgg taatgaactg cacgaacgtt atgcgaacca gagcaatatc
    14101 actattatcc acaacccaga ttatgcgcag ggtttactaa catcagtaaa ggccgcagta
    14161 cccgcagtgc aaacagaaca ttgttttctc acccacggtg acatgccaac cctcaccatc
    14221 gatattttta gaaaaatctg gtcgttacga aatgatggcg caatactgcc actccataat
    14281 ggcatccccg gccatccgat tttagtgtca aaaccatgct tgatgcaggc aatccaacga
    14341 cccaatgtca ccaatatgcg tcaggcactc cttatgggag ctcattattc cgtcgagata
    14401 gaaaatgcag aaataatttt agatattgat actccggacg actttattac agcgcaaaaa
    14461 aggtatacta aaatttagaa aatgtagtta aacgattgcg ttcaaatatt taatctctcc
    14521 agcactcttt atcataatga taaggaatca ctgattttga gaaaagagaa aaagtgataa
    14581 atgggctgac aataataatt ttcaggagtg attttaattc tcatttggca cctgtgagtt
    14641 cattcacaaa aagcctttaa gtttcgagcc gcctcacatt tttttatatt tcccgccaaa
    14701 cctggcaaga gtggtgcgat tgttgctcta tccccctaaa ccaccggatt tctcaacacc
    14761 ggtcactcaa tgatatctgt ataagctaag gagagggtta tgggggatat tatgcgtccc
    14821 attccgtttg aggaactttt gacgcgcata tttgatgaat accaacaaca acgctcaatc
    14881 tttggtattc ccgagcaaca gttttactca cctgtaaaag gtaaaactgt tagcgtcttc
    14941 ggtgaaacct gtgccactcc cgtcggccct gccgctggcc cgcacacgca gctcgcgcaa
    15001 aatattgtca cttcctggct gactggcgga cgcttcatcg aactaaaaac cgtccaaatt
    15061 cttgaccgcc tggagctgga aaagccctgt atcgatgccg aagacgagtg ctttaacacc
    15121 gaatggtcta ccgaatttac cctgcttaaa gcctgggatg aatacctcaa agcctggttt
    15181 gccctgcacc ttctcgaagc gatgttccag ccttctgatt ccggtaaatc gttcatcttt
    15241 aatatgagcg tcggttacaa cctcgaaggt attaagcaac cgccaatgca acagttcatc
    15301 gacaatatga tggacgcatc tgaccatccg aaattcgcgc aatatcgcga tacgctgaat
    15361 aaattactcc aggatgacgc atttttagcc cgccacggat tgcaggaaaa acgcgaaagc
    15421 ttgcaagcct tacccgctcg catccccacc agtatggtgc agggcgtcac cctctccacc
    15481 atgcacggct gtcctccgca tgaaatcgaa gccatttgcc gctacatgct ggaagaaaaa
    15541 gggctcaaca cctttgtgaa acttaacccg accttactgg catacgcgcg tgttcgtgag
    15601 atcctcgatg tctgcggttt cggttacata ggcttaaaag aagagtcatt tgatcacgac
    15661 ctcaagctga cgcaagcact ggaaatgctg gaacgcctga tggcactggc aaaagaaaaa
    15721 tcactcggct ttggcgtaaa actgactaac actctcggca ccatcaacaa taaaggcgca
    15781 ctgcctggtg aagagatgta tatgtcaggc cgtgcgctgt tcccgctctc catcaatgtt
    15841 gcagcagttc tctctcgcgc ctttgacggc aaactgccca tttcttattc cggtggtgcc
    15901 agtcagctga ctatccgcga tatttttgat acaggtattc gccctattac tatggcaacc
    15961 gacctgctga aacctggcgg ctatttgcgc ttaagtgcct gcatgcgcga gctggaaggc
    16021 tccgacgcct ggggacttga ccatgttgac gtcgaacgac tgaacagact ggcagcagat
    16081 gcgttaacca tggaatacac ccagaaacac tggaagccag aagagcgtat tgaagtggca
    16141 gaagacctgc cgctgaccga ttgctacgtt gccccctgtg ttactgcctg cgctatcaag
    16201 caagatattc cggaatacat ccgtctgctt ggcgaacacc gctatgccga cgcgctggaa
    16261 ctcatctacc aacgcaacgc tctgcccgcc attaccggtc atatttgcga tcaccagtgc
    16321 caatacaact gtacccgcct ggattacgac agtgcgctga atatccgcga actgaaaaaa
    16381 gtcgcgctgg aaaaaggttg ggatgaatat aagcaacgct ggcacaaacc agccggttct
    16441 ggttcacgcc atccggttgc cgtgattggt gcaggtccgg cgggtctggc agcaggttac
    16501 ttccttgcca gagcgggcca tccggttacg ctgtttgaac gcgaagccaa tgcgggcggc
    16561 gtggtgaaaa atatcattcc tcagttccga attccggctg agttaattca gcacgatatc
    16621 gattttgttg ccgatcacgg cgtgaaattt gagtatggct gctcacccga tttgaccgtt
    16681 gaacagttaa aaaatcaggg cttccactat gttctgattg ccaccggcac tgataaaaat
    16741 agcggtgtga aactggcggg tgacaaccaa aatgtttgga aatcactccc cttcctgcgt
    16801 gaatacaaca agggtacagc gctcaagctg ggcaaacatg tggtcgttgt cggggcgggt
    16861 aacaccgcaa tggactgcgc tcgtgcggcg ttacgcgttc caggcgtaga aaaagcaacg
    16921 atcgtttacc gtcgttcact gcaagagatg cccgcatggc gcgaagagta tgaagaagcg
    16981 ttgcacgacg gcgtggagtt ccgtttcctg aataatccgg aacgtttcga tgctgatggc
    17041 accttaacct tgcgcgttat gtcgcttggc gaacccgatg agaaaggccg tcgccgtccg
    17101 gttgaaacca acgaaacagt aacactgcat gtagacagcc tgatcaccgc cattggtgaa
    17161 cagcaggata ctgaagccct gaatgcgatg ggcgtgccgc tggacaaaaa cggctggcca
    17221 gacgtcgacc acaatggcga aactcgtctg actgacgtct ttatgatcgg cgacgtacag
    17281 cgcggaccat cctccattgt cgctgctgtc ggaaccgcgc gtcgggcgac cgatgccatc
    17341 cttagtcggg aaaatatccg ttcccaccag aacgataaat actggaacaa cgtcaatccg
    17401 gcggaaatct atcaacgtaa aggcgatatc tctatcactc tggtgaacag tgacgatcgt
    17461 gacgcgtttg tcgcccagga agccgctcgc tgcctcgaat gtaactacgt ttgcagcaag
    17521 tgtgtggatg tctgcccgaa ccgcgccaac gtctccattg cggtcccagg cttccagaat
    17581 agattccaga cgctgcacct cgacgcttac tgtaacgaat gcggcaactg cgctcagttc
    17641 tgtccgtgga acggtaaacc atacaaagac aaaatcaccg ttttcagcct ggcgcaagac
    17701 tttgataaca gcagcaaccc aggcttcctt gtggaagatt gccgggtacg agtacgactg
    17761 aataaccaaa gctgggtgtt aaacatcgac agcgaaggtc agttcaacaa cgtaccaccg
    17821 gagctgaacg atatgtgccg catcatcagc catgtccacc agcatcatca ttatctgctg
    17881 ggccgcgtgg aggtgtaatc atgttgattc tgaagaatgt caccgcagtg cagttacacc
    17941 cggcgaaagt gcaggaaggc gttgatatcg ctatcgaaaa tgatgtgatt gtcgctatcg
    18001 gcgatgccct gacgcaacgc taccccgatg ccagctacaa agagatgcat ggtcggattg
    18061 tgatgccggg aattgtctgc tcgcacaacc atttttactc ggggctttcc cgcggaatta
    18121 tggcaaacat cgccccctgc ccggatttca tctcaacgct gaaaaatctc tggtggcggc
    18181 tcgatcgcgc ccttgatgaa gagtcgctct attacagcgg gctgatttgt tccctggaag
    18241 cgattaagag cggatgtaca tcggttatcg atcaccatgc ctctccggcg tatatcggcg
    18301 ggtcgctctc cacattgcgc gacgcatttt taaaagttgg cctgcgcgcg atgacctgtt
    18361 ttgaaactac tgaccgtaac aacggcatca aagagttgca ggaaggtgta gaagaaaaca
    18421 tccgcttcgc ccgtcagatt gatgaggcga agaaagcagc aaccgagccg tatctggtgg
    18481 aagcacatat cggtgctcac gcgccgttta ccgtgccaga tgccggtctg gagatgctac
    18541 gtgaagccgt gaaagccaca ggccgtggtt tgcatattca cgctgcggaa gacctttacg
    18601 acgtttccta cagtcaccac tggtacggca aagacctgct ggcacgacta gcgcaattcg
    18661 atctcatcga tagcaaaacg ctggtcgctc atgggctgta cttgtcgaaa gatgacatcg
    18721 ccctactcaa tcagcgcgat gcgttcctgg tgcataacgc ccgttcaaac atgaacaacc
    18781 atgtcggcta caaccatcac cttagcgaca tccgcaatct ggcgttggga acggacggca
    18841 ttggttcgga catgtttgaa gagatgaaat ttgccttctt taaacatcgc gatgcgggtg
    18901 gtccgctgtg gcctgacagt tttgccaaag ccctgactaa cggtaacgaa ctgatgagcc
    18961 gcaactttgg cgcgaaattt ggacttctgg aagccggtta caaagctgat ttaaccattt
    19021 gcgattacaa ctcaccgacg ccgctgctgg cagacaatat cgccgggcat atcgctttcg
    19081 gtatgggctc aggcagcgtt cacagcgtga tggtcaatgg tgtgatggtc tatgaagacc
    19141 gtcagtttaa cttcgattgc gattccattt atgcacaagc cagaaaagcc gctgccagta
    19201 tgtggcgtcg gatggatgcg ctggcataaa tgacagatgc cctcttcccg cagggaagag
    19261 ggttaaataa ggaaagaaga tgattgaaca atttttcagg cccgactctg tcgaacaggc
    19321 gctggaactg aagcgccgct accaggatga agccgtctgg ttcgccgggg gcagcaaact
    19381 caatgctaca ccaacccgta ccgataaaaa gattgccatt tccttacagg atctggaact
    19441 ggactgggtt gactgggata acggtgcact gcggattggc gcaatgtctc gcttgcagcc
    19501 actgcgtgat gcgcgattta ttcctgcagc gctgcgtgaa gccctcggtt ttgtttactc
    19561 acgccatgtt cgtaatcagt cgaccattgg tggtgaaatc gccgcccgcc aggaagagtc
    19621 ggtgctgctt cccgtcctgt tggcactgga tgctgaactg gtttttggca acggcgaaac
    19681 gctgtcaatc gaggactacc tggcctgccc atgcgatcgc ctgctaaccg aaattatcat
    19741 taaagatccg tatcgcacct gtgcgacccg caaaattagc cgttctcagg caggtttaac
    19801 cgtcgtgacg gcagccgttg caatgacaga ccacgacggt atgcgaattg cgctggatgg
    19861 cgtcgccagt aaagcactgc gtctgcatga tgtcgaaaaa caaaatctgg aaggcaatgc
    19921 acttgaacag gctgtcgcca acgccatttt cccgcaggaa gatttgcggg gcagcgtggc
    19981 ctataaacgc tatatcacgg gagttctggt agccgacctg tatgccgact gccaacaggc
    20041 tggggaggaa gccgtatgat catccacttt actttaaatg gcgcgcctca ggagctaacc
    20101 gttaatccag gcgaaaacgt gcaaaagctg ttgtttaaca tgggaatgca ctctgtacgc
    20161 aacagtgatg atggcttcgg gtttgccggt tctgacgcaa taatctttaa cggtaatatc
    20221 gttaacgcgt ccttgcttat tgccgcacag ttagagaagg cagatattcg taccgcagaa
    20281 tctttgggca aatggaacga gttaagtctg gttcaacagg caatggttga tgttggcgtg
    20341 gtgcagtctg gttataacga tccagctgca gctctgatta tcaccgatct tctcgatcgc
    20401 atcgccgcac ctacccgcga agagatcgac gacgcgcttt ctggtttgtt cagccgcgat
    20461 gcaggctggc aacaatacta tcaggtcatt gaactggcgg ttgcacgtaa aaataatccg
    20521 caggccacca ttgatatcgc tccgactttc cgtgacgacc ttgaagtcat tggcaagcat
    20581 tatcctaaaa ctgatgccgc gaaaatggtg caggcgaaac cctgctatgt tgaagaccgc
    20641 gtaacggctg acgcctgcgt cattaaaatg ttacgtagcc cacacgctca cgcactgatt
    20701 actcatctgg atgtcagcaa agctgaagcc ttaccgggcg tcgttcacgt tattactcac
    20761 ctgaattgcc cggatatcta ctataccccg ggtggtcaga gcgcaccgga accgtcaccg
    20821 cttgaccgcc gtatgttcgg caagaaaatg cgtcacgtcg gcgatcgtgt tgctgcggtc
    20881 gtcgcagaaa gtgaagaaat tgcgctcgaa gcattgaagc tcatcgacgt tgaatatgaa
    20941 gtgcttaagc cggtaatgtc gatcgacgaa gcaatggcgg aagatgcgcc tgtcgtgcac
    21001 gatgaaccgg tggtgtatgt tgctggtgcg ccagatactc tggaagacga taacagccat
    21061 gcagcccagc gcggcgagca tatgatcatc aacttcccga tcggttctcg ccctcgcaaa
    21121 aatatcgccg ccagtattca tggtcatatt ggcgatatgg acaaaggctt tgccgatgcc
    21181 gatgtgatca ttgagcgaac ctataactca acgcaggcac agcagtgccc gactgaaaca
    21241 catatctgct ttacccggat ggacggcgat cgtctggtta tccacgcctc cacccaggta
    21301 ccatggcact tacgccgcca ggtcgcgcgc ctcgtaggca tgaaacagca taaagttcat
    21361 gtcattaaag agcgagttgg cggcggtttt ggctccaaac aggacatcct gctggaagaa
    21421 gtgtgcgcat gggcaacctg cgtgaccggg cgtccggtac tgttccgcta cacccgtgaa
    21481 gaagagttta ttgctaacac ctctcgtcac gtcgcgaaag tcaccgtcaa actgggagcg
    21541 aaaaaagatg gtcgcctgac ggcagtgaag atggatttcc gcgccaacac tggcccttac
    21601 ggcaaccact cactcaccgt accgtgtaac ggaccggcgc tgtcgctgcc gttatatccg
    21661 tgcgataacg tcgatttcca ggtcaccacc tactacagca acatttgccc aaatggtgct
    21721 tatcagggtt atggcgcacc gaaaggtaac ttcgctatca ccatggcatt agcggaactg
    21781 gctgaacagt tacagatcga ccaactggaa attatcgaac gtaaccgggt acacgaaggg
    21841 caagagctga aaattctcgg tgcaatcggt gaaggtaaag cgccgacctc cgttccttcc
    21901 gccgccagct gcgcactgga agagatcctg cgtcagggtc gcgagatgat ccaatggtct
    21961 tcaccgaaac cgcaaaatag tgactggcac atcggtcgcg gcgtcgccat tatcatgcag
    22021 aaatcgggta tcccggatat cgatcaggct aactgcatga tcaaactgga atcggacggc
    22081 acctttatcg ttcattctgg cggtgcggat attggtactg gtctggatac tgtagtgacg
    22141 aaactggcag cagaagtgct gcactgccca ccgcaggacg tgcatgttat ctccggtgat
    22201 accgatcatg cgttgtttga taaaggcgca tatgcctcgt ccggtacttg cttctcgggt
    22261 aacgcggcgc gtttggcagc ggaaaatctg cgggagaaaa ttctgttcca cggcgcgcaa
    22321 atgttgggtg agccagtggc agatgttcaa ctagcaacgc cgggcgtcgt gcgcggcaag
    22381 aaaggcgaag ttagtttcgg ggatattgcc cataaaggcg aaaccggcac cggctttggt
    22441 tcactggtgg gaactggcag ttatatcacg cctgatttcg ctttcccgta tggcgcaaac
    22501 ttcgctgaag ttgccgtcaa cacgcgtacg ggtgaaatcc gcctggataa attctacgcc
    22561 ttgctggact gcggtacacc ggtcaatcca gagttagcgt tgggacaaat ctacggtgcc
    22621 accctgcgtg ctatcggcca cagtatgagc gaagagatca tttataacgc cgaaggtcac
    22681 ccgttaacgc gtgatttacg cagttacggc gcaccgaaaa ttggtgacat tccgcgtgat
    22741 ttccgcgctg tgttggtgcc gagcgacgat aaagtcggcc cgttcggggc gaaatcgatc
    22801 tcggaaattg gtgtaaatgg cgcagctccg gcgattgcta ccgcaattca cgatgcctgc
    22861 ggcatctggt tacgcgaatg gcatttcaca ccggagaaaa tactcaccgc gctggaaaaa
    22921 atataagtaa taaatggccg cttcggcggc ctgtttctct gtttgaacga tgactaaata
    22981 gtgactgcga aaatgagata agccgttctc aaggcgtcct gcgccccaaa ttcgtgggaa
    23041 tttattttac taattcagat gatcaaattt actttaaaag gagtgaaggg atgtctgata
    23101 taaaccatgc aggttctgac cttatatttg aactggagga tcgccctccc tttcatcagg
    23161 ctctcgttgg tgccattacc catctgttgg caattttcgt tccgatggta acccccgcgt
    23221 taatcgtggg tgcggcctta cagctttccg ctgaaacaac cgcctatctt gtttctatgg
    23281 cgatgatcgc ctctggtatt ggtacctggt tacaagtaaa ccgctacggc atcgtcggtt
    23341 ctggcctact ctcaattcag tcagtcaatt tttcatttgt tacggtcatg attgcgctgg
    23401 gcagcagcat gaaaagcgac ggttttcacg aagagttaat catgtcgtcg cttctcggcg
    23461 tctccttcgt tggcgcattt ctggttgtcg gatcttcatt tatcttgccc tatttacgtc
    23521 gggttattac gcctaccgtc agcggtattg tggtactgat gatcggctta agcctgatta
    23581 aagtcggcat tatcgatttt ggtggaggat ttgcagccaa aagcagcggt acgttcggca
    23641 attacgaaca tctcggcgtt ggtttattgg ttttaattgt ggtgatcggc tttaactgct
    23701 gtcgcagtcc gttgctacgc atgggaggga tcgccattgg gctatgtgtc ggctatatcg
    23761 catcgttatg cctgggcatg gtggatttca gcagtatgcg caatttgccg ttaatcacca
    23821 tcccgcatcc gttcaaatac ggctttagtt ttagcttcca tcagttcctg gtggttggca
    23881 cgatttatct gcttagcgtg ctggaagcag tcggcgatat caccgccacg gcaatggttt
    23941 cccgccgccc cattcagggg gaagagtatc agtcccgact gaaaggcggc gtgctggcag
    24001 atggtctggt ttctgttatc gcctccgctg tcgggtcatt acccttaacc acgtttgcgc
    24061 aaaataatgg ggttattcag atgactggcg tcgcttcacg ttatgtcggg cgaaccatcg
    24121 cggtaatgct ggttatcctc ggtttgtttc cgatgattgg cggcttcttc acgaccattc
    24181 cctcggcagt tctgggaggc gcaatgacgt tgatgttttc catgattgcc atcgctggga
    24241 ttcgcataat catcaccaac ggtttaaagc gccgagaaac acttattgtc gccacttctt
    24301 taggtttagg acttggcgtc tcctacgatc ccgaaatttt taaaatattg ccagcctcta
    24361 tttatgtact agttgaaaac cctatttgtg ctggcgggtt aactgcgatt ttattaaata
    24421 ttatcctccc tggtggctac cgacaggaaa acgttctgcc tggtattacc tcagcggaag
    24481 agatggatta acagtaaagg agtcaatgat gtcaggagaa cacacgttga aagcggtacg
    24541 aggcagtttt attgatgtca cccgtacggt cgataacccg gaagagattg cctctgcgct
    24601 gcggtttatt gaggatggtt tattactcat taaacaggga aaagtggaat ggtttggcga
    24661 atgggaagac ggaaagcatc aaattcctga cactattcgc gtgcgcgact atcgcggcaa
    24721 actgatagta ccgggctttg tcgatacaca tatccattat ccgcaaagtg aaatggtggg
    24781 ggcctatggt gaacaattgc tggagtggtt gaataaacac accttcccta ctgaacgtcg
    24841 ttatgaggat ttagagtacg cccgcgaaat gtcggcattc ttcatcaagc agcttttacg
    24901 taacggaacc accacggcgc tggtgtttgg cactgttcat ccgcaatctg ttgatgcgct
    24961 gtttgaagcc gccagtcata tcaatatgcg tatgattgcc ggtaaggtga tgatggaccg
    25021 caacgcaccg gattatctgc tcgacactgc cgaaagcagc tatcaccaaa gcaaagaact
    25081 gattgaacgc tggcacaaaa atggtcgtct gctatatgcg attacgccac gcttcgcccc
    25141 gacctcatct cctgaacaga tggcgatggc gcaacgcctg aaagaagaat atccggatac
    25201 gtgggtacat acccatctct gtgaaaacaa agatgaaatt gcctgggtga aatcgcttta
    25261 tcctgaccat gatggttatc tggatgttta ccatcagtac ggcctgaccg gtaaaaactg
    25321 tgtctttgct cactgcgtcc atctcgaaga aaaagagtgg gatcgtctca gcgaaaccaa
    25381 atccagcatt gctttctgtc cgacctccaa cctttacctc ggcagcggct tattcaactt
    25441 gaaaaaagca tggcagaaga aagttaaagt gggcatggga acggatatcg gtgccggaac
    25501 cactttcaac atgctgcaaa cgctgaacga agcctacaaa gtattgcaat tacaaggcta
    25561 tcgcctctcg gcatatgaag cgttttacct ggccacgctc ggcggagcga aatctctggg
    25621 ccttgacgat ttgattggca actttttacc tggcaaagag gctgatttcg tggtgatgga
    25681 acccaccgcc actccgctac agcagctgcg ctatgacaac tctgtttctt tagtcgacaa
    25741 attgttcgtg attatgacgt tgggcgatga ccgttcgatc taccgcacct acgttgatgg
    25801 tcgtctggtg tacgaacgca actaataata aaactttaac atcctcgtga ggacatcatt
    25861 atgtctggag acatcctaca aacaccggac gcaccaaagc cacagggcgc gctggataat
    25921 tattttaaaa ttaccgctcg tggcagtacc gttcgtcagg aagtactggc tggcttaacg
    25981 acctttctgg ccatggttta ttccgttatc gtcgttccgg gaatgctggg caaagcaggt
    26041 tttcctcccg cagctgtgtt tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg
    26101 atggggttgt gggccaattt gccaatggcg attggttgtg cgatttcctt gacggcattt
    26161 accgcattca gtctggtact cgggcaacaa attagcgttc ctgtcgcact gggcgcggtc
    26221 tttctgatgg gcgtcatctt caccgctatt tccgtaaccg gtgtgcgtac ctggatctta
    26281 cgtaatttac cgatgggtat cgctcacggt acaggtatcg gtatcggcct gtttctgctg
    26341 ctgattgctg ctaacggtgt gggtatggtt atcaaaaacc cgattgaagg cttgccagtg
    26401 gcgctcggtg cgtttacctc cttcccggtg atgatgagct tgctggggct ggcggtcatc
    26461 ttcggtctgg agaagtgccg cgtacccggc gggatcttgt tggtgattat cgcaatttcg
    26521 attatcggct taatctttga cccagcggtg aaataccacg gtctggtggc gatgccaagt
    26581 ctgactggcg aagatggtaa gtctctgatt ttcagcctcg atattatggg cgcactccag
    26641 ccaaccgtac ttccgagtgt actggcattg gtgatgaccg cagtgttcga cgccactggc
    26701 accatccgtg ccgtcgccgg tcaggcgaat ttgttggata aagacaacca gatcatcaat
    26761 ggcggcaaag ccctgaccag tgactcagta agttcaatat tctccggtct ggtgggcgca
    26821 gcgcccgcag cggtttatat cgaatcagcg gcaggaaccg ccgccggggg taaaacaggg
    26881 ttgaccgcaa ccgtagtggg ggcgttattc ctgctgattc tgttcttatc accgctgtca
    26941 tttttgatcc ctggttacgc cactgcaccc gctctgatgt acgtgggttt gctgatgtta
    27001 agtaacgtct cgaagctgga tttcaacgat tttattgacg caatggctgg cctggtgtgt
    27061 gccgtgttca tcgttctgac ttgtaatatc gttaccggta ttatgctggg ctttgtgaca
    27121 ctggtcgtag gccgtgtctt tgctcgcgaa tggcaaaagt tgaatattgg tacggtgatc
    27181 attactgccg cactggtcgc attttacgcg ggtggttggg caatctaata gattctccgc
    27241 gcctttcagc gcggagattt ctttcacaga ggattctcac cgctggcaac aatacgctgc
    27301 tggcggactc gcctgagttc tctttcagta agcaactgca gcgcctgtgt cgggcaagat
    27361 tcaacacagg ctggcccctc ttcccgctgt tcacagagat cgcatttcac aatttgttgt
    27421 cgggtatccc ccggcaacga ctgaatggtg atcattccaa acgggcacgc actgacgcaa
    27481 ctctgacagc caatacaacg ggcagaattg gtctgcacca cctgctcgcc catcgtcagc
    27541 gcccccacgg ggcaagcgcc aacacaaggg gcgttttcac actgatggca catcaccggc
    27601 gcactgatgc tgtccagtcg ctgtaccttc agccggggta aaaagacatc ggcattcaac
    27661 tcctgttctg aagggtgagc gaccacacag gctacttcac aagtacggca gccaatacag
    27721 tcagccggat taacgataat taacgatttc atcacgatgc cttcgtatca aacagagtta
    27781 acatatcgcg cgccgcctgc cttcctgcgg ccattgcagt gacaaccaga tccgcgccat
    27841 gaactgcatc accaccagca aagacttttt tcagatgcgt ctgggtaggt aaatacccga
    27901 cgtcaccggt ttgaatcagg ccccatttat cgagtttaat tccgctgccc tgcaaccacg
    27961 gcatggcatg cgcctggaaa ccaaaggcca taatcagaac atcggcgggc aattcaaact
    28021 ctgaacccgc taccggacga ggacgacggc gaccatccgg ccccggctca cccatggcgg
    28081 tacgaatcag gcccaccgca gttaaacgcc catcttcgtc acaagcgata tattgcggtt
    28141 gaacattgaa ctgaaactcg acaccttcct cgcgcgcatt aaccacctct ttgcgcgagc
    28201 ccggcatact gacttcatca cgacgatacg cgcaggtcac gctggcggca ttgaggcgga
    28261 tggaagtccg caaacaatcc attgtcgtat cgccaccgcc caataccacg actcgcttac
    28321 cttccacgtc cgtcagcgga tactcttcag actccggcaa tcccatgagc tggcgggtat
    28381 gggcagtcag gaacggtaga gcctgaatga caccgggcgc atcttcatgc ggcagatctg
    28441 ctcgcatcat cccgtaagtc cccacgccga tgaaaactgc atcatattca gaagttaaat
    28501 cgctaaaggt aatatcgcgg ccaatttcac agttaagatg aaagtcgatt cccattgcgg
    28561 tgaatatctc tcgccgctgg cttaataccg ttttatcgag tttgaaagga ggaatgccaa
    28621 aagtcagcat accgccaatt tctggatggc gatcaaagac atcaacctga actcccgcgc
    28681 gcgccagaat atcagcacac cctaaccctg ctggcccagc gccaatcacc gccacttttt
    28741 cgctacgggg aacaactttg ctgacatcgg gacgccagcc catcgccagc gcggtatcgg
    28801 tgatgtagcg ttccagatta ccgatagaga ctgcgcccga gtgatctttc aaagtacatg
    28861 cgccttcaca aagacggtcc tgtggacata ccctgccgca aatttctggt aaggaactgg
    28921 tctggtggca aagttctgcc gcttcaataa tctttccttc ctgtaccaga cggatgtaat
    28981 ccggaatagc gttatgcagc gggcaatgcc agttgcagtt agctttttcg gcacaataaa
    29041 cacagcggtc actctcataa gtcgcttgca gtggatccag cccgcaatat atttcgccaa
    29101 agtgggtttt ccgttcactc gctgaaattt tatctgcgcc tttacgcgag ttaacgggga
    29161 gcaacgctgc actgcgagat ggctgagcat ctgatgacgc ttttcctgct gccgttttgc
    29221 gctggcgagc cacctttatc cactgtaacc ctttatcgtc catcagtcgt aacgcctgtg
    29281 ttgggcagac ttcaatacag gcttgcgtgc cggaactgcg ctggttacaa aggtcgcatt
    29341 tctgcgcaat cgtatcgacc atctcaacga cgccaaaggg gcaagcgatt gcgcatcttt
    29401 tacagccaat acatttttgc tcgtccagtt gtacgctatc ggactggaaa gtcagagcat
    29461 taaccggaca agccgtaacg caaggggcat tgttgcagtg atggcaggcc accggattcg
    29521 cagcctggcc tttccctaca acgtggatac gcggtcgaaa gtcactgtga ctcagcggcc
    29581 agttttcttg attgtgtgcc accgcacagg caatttcaca agcatgacag cctatacatt
    29641 ccgcagcttc agcagcgata aacttattca tttgcatccc tttcatttaa tgagttatgt
    29701 cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac cgttttaatt
    29761 ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt cattatttca
    29821 acattgccga ccaaaagagc gctatccaga taaaaacgat taaatgcgag agtgcgacat
    29881 gccagaatga ttaatgaaat atcaatatga gaaaattaca gataatttca ttgtcaggtt
    29941 ctcttctcat tttcgtggtt taaccacgat tatgtgattg cctccgcaat tcctgtctct
    30001 aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa
    30061 cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga gcgccataga
    30121 ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt
    30181 atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc
    30241 agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagaag ctattgcgat
    30301 gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat
    30361 cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac
    30421 accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac
    30481 tatcgccgcg ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt
    30541 attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt
    30601 gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt
    30661 aggtatctcc tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt
    30721 tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat
    30781 gaatgaagtc aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat
    30841 gtcgtttggt atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat
    30901 catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa
    30961 actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg
    31021 cggcacgttt aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt
    31081 gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg
    31141 catggtgcca aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc
    31201 tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa
    31261 ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac
    31321 tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgttgctgca
    31381 tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct ttaatggcta
    31441 tcagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac
    31501 agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga
    31561 attttttaat gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca
    31621 tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt
    31681 aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctcactt
    31741 ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga
    31801 aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct
    31861 ggaaaagtat gccgcacaca cggcagacac ccgcttacat cgcgcgttct ccagttggct
    31921 gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc
    31981 tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca acgaagacgc
    32041 ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta
    32101 tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg aagtgtgtcc
    32161 ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta
    32221 tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt gggcgttcag
    32281 tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt ctgctttcgc
    32341 gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt
    32401 ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca
    32461 gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca
    32521 gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg
    32581 caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt
    32641 taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt
    32701 cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa
    32761 tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc
    32821 tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca
    32881 gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt
    32941 cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa
    33001 tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg
    33061 cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt
    33121 cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct
    33181 tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg
    33241 tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt
    33301 ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt
    33361 taaaggtgtt acgggattca tcgttagaga tgagatccaa ataacgctga cgatagcgcg
    33421 cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca
    33481 gacgcagctc ggtgcagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt
    33541 tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg
    33601 gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga
    33661 aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt
    33721 tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg
    33781 tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg ttcgccagct
    33841 tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt
    33901 gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat
    33961 ccaggctgcc gtccagcacg gcctgcgtgt tgcgggtttc taccccggta cgcagatctt
    34021 taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg atgtcggatt
    34081 tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct
    34141 tcgctttcat ctgcttcatg gcctggtctt tgttcttgtg ctgggaacgg tcgttctggc
    34201 attgggtcac gatcccggtt gggatgtggg taatacgcac cgcagattcg gtacggttaa
    34261 cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga
    34321 tttcgatatc aatatcatca tcaacttccg ggtaaacaaa cgcagagctg aacgatgtgt
    34381 ggcgacgacc gccggagtca aacgggcttt tacgcaccag gcggtgaacg ccagtttctg
    34441 tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg
    34501 ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat tcagcccagc
    34561 gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg
    34621 cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata cggcggaact
    34681 caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt
    34741 cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt
    34801 tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct
    34861 gtgcgcgttc gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca
    34921 gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg
    34981 aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg
    35041 agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag
    35101 agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg
    35161 cgaaactcgt tgatatcgag cttataagcc agttgcactt cacgcacgcc gttatccggc
    35221 cagagggcgg tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt
    35281 tcgaccatca ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga
    35341 ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcaactgc
    35401 gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc
    35461 aatagcgaag ggtccagcca ctctgtaacc agttcgccaa accgttgttg aaagagttcg
    35521 aatttatcct cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttcagtatc
    35581 atgccagggt agagtgtgtc taatcgctcc agtgcatcac gcatatgcag cccctgaatg
    35641 gagcgacctg aacctttcag cgtaccatca cctgctggcg caaaggcgat aaccggacgg
    35701 tgaaaacgct ctttgatgcg cgaagccaga ataccgacaa cgccctgatg ccattcgggg
    35761 tgatacattg ccagcccgcc gggtagcgtg tcgcgacttc gctccagttt ctcgcacagg
    35821 gtcagggctt caacttgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg
    35881 agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacaccg
    35941 acggacatat cgtccagtcg cccggcagca ttgagacgtg gccccagcgc aaaacctaaa
    36001 tcgctggcgg cgagtttttg cgcatcgcga tttgccactt ccagcagcgc tttaatcccc
    36061 ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta
    36121 gcgtccagcg gcacgacgtc cgccactgtt cccagcgcga ccagatccag cagttctgcc
    36181 aggttaggaa ttgcgatgcc acgctcatca aaccagccct gatcgcgcaa aaaggtgcgc
    36241 agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta
    36301 cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc gcctggcaaa
    36361 tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgtacaac ccccgcatgg
    36421 gaggaaatac cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg
    36481 accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata
    36541 ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg
    36601 gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc
    36661 tcaacggcct tttcgacgcc gctcagttgc tgccagggca acataccttt aacactgcgt
    36721 tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag caagggaggc
    36781 aattcagcgg gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt
    36841 ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatct
    36901 ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac
    36961 cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg
    37021 ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac
    37081 accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa
    37141 gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc
    37201 agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat
    37261 aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat
    37321 tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt ttaccatcat
    37381 cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct acgggcgcgg
    37441 gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat
    37501 cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca
    37561 taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc
    37621 gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca
    37681 gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt
    37741 gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcaca gcataatgtt
    37801 taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca
    37861 cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc
    37921 agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta ccaatgaccc
    37981 gcactacgcc ctgacgcagg ctgatatcac tcattgtcag tccgaccagt tcagagacac
    38041 gcagcccggt agcatacaac acttcaagca tagctttatc gcgtagctcc agtggctgat
    38101 caattaatgg tgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac
    38161 gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc
    38221 gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt
    38281 tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg
    38341 ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag
    38401 cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga
    38461 tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc
    38521 ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg
    38581 agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat
    38641 ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa
    38701 ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg
    38761 ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg acgacctgaa
    38821 ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat acggcgagtg
    38881 gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt
    38941 cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg
    39001 gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg
    39061 tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca
    39121 ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca
    39181 aaatccgccg taaatcccgc cattcagctt cgtccatgct gtcggctgcc agccataaat
    39241 gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg
    39301 gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc
    39361 gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca aacaccacca
    39421 gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg agtaaaataa
    39481 cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca
    39541 aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccatcatt
    39601 tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg
    39661 tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca
    39721 tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa
    39781 tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc
    39841 cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca ccatcgcgat
    39901 cgcgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc ctcgcagaag
    39961 gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt
    40021 aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc ctccccgtca
    40081 gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc
    40141 caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag
    40201 ccagatgaca gaaaatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg
    40261 gagcaattta cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt
    40321 gcgtgaaccg cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc
    40381 gccagacgac gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc
    40441 aaatctcttt agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac
    40501 tttgctatgg tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc
    40561 taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct
    40621 ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg ggcagtttat
    40681 cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac
    40741 cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg cgctctggtt
    40801 gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg
    40861 cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt
    40921 cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc gcgtacgcga
    40981 cgatgcgaat acattgcata tcgagccgct gccgtattcg ctcgaagagt aaatctcttt
    41041 atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt
    41101 gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgaatc
    41161 cggcagttgt gctccgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacaaaggc
    41221 atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc
    41281 cgagcacgaa gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa
    41341 tcaccccgag cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga
    41401 gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc agataggtca
    41461 ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata cccagcaatg
    41521 ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca
    41581 aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca
    41641 gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca
    41701 ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat
    41761 ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac ccaatgccgt
    41821 gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct
    41881 gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca gcgtaactga
    41941 gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac taatctccta
    42001 aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat ttaaagacat
    42061 ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat gacctttatc
    42121 agttcagtca gggtcatatt ttcctgctct gcatgttttt cagtcagcgt atccagcgtt
    42181 acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg
    42241 acacgaagca catcacccgt tttgaagtgt gattcagact cgtcgcggat ggtgatggtt
    42301 ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc
    42361 atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga aaaaactcac
    42421 cttaccgaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg aaggcggctg
    42481 gaacaaaggc ggaaaagggc cgagcatttg tgacgttctg accggtggcg cacacggcgt
    42541 gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc atgaagccgt
    42601 tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg
    42661 ttttcggact tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa
    42721 cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat acaacatcga
    42781 accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag
    42841 ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg tgtttgaacg
    42901 ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc agcgtaactg
    42961 gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc atgagaaccc
    43021 ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc tggcggtgaa
    43081 agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct
    43141 ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg
    43201 ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt tgaacgagtg
    43261 ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac tgcgtgaagg
    43321 cacctgcgat tatcttggtt tcaggtatta catgaccaac gcagtgaagg ccgaaggcgg
    43381 caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg ttaaagcgtc
    43441 tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg aactgtatga
    43501 gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg acaaagtgga
    43561 agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc atatcgaaga
    43621 gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac cgtggggctg
    43681 catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct ttatctatgt
    43741 gaataaacat gacgacggtg ctggcgatat gtcgcgttca cgtaagaaga gctttaactg
    43801 gtaccaagag gtgattgcca gcaacggcga gaagctttaa gtcgatgaag taccggatgc
    43861 aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg ccaaatcgat
    43921 aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg cctgcgcgac
    43981 ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat cgacgcgtcc
    44041 aggctcgccg ccgctggcgt gcatttcggt ataaataaac cctggccgca cgcagttaac
    44101 gcggatcccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat caatcgcccc
    44161 tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg ccaccgaaga
    44221 gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt ttaccgcctc
    44281 gcggcagcag agaaaatatc ccgtcacgtt ggtggaaagt actcggttga ttcgctctgc
    44341 ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga ccagcgctgc
    44401 tagcggttca tcgtgctgat cgattgctgt aaacatcgca acgacctggt tttcgtcgct
    44461 gatatccgcc tggagcacga atgctttgcc accggcttgc gttattaagt tcatcacttc
    44521 ctgcgccgcg tggaggtttt gctgataatt aaccgccacc gtataccctt cttgcgccaa
    44581 cagtaatgca gttgcccgcc cgatgccgcg actgccaccg gtcacaagtg ctatagccat
    44641 ttgtttttcc gagaaaaatt cattcgttga cgtatctttg agattgctaa caaactggct
    44701 caatcttcaa tgttggcacg cttttccccc tcgccctttc agggagaggg ccggggtgag
    44761 ggtaaatatt cgcgccagtg ccggcctgtt cccctcaccc tctccccaaa ggggcgaggg
    44821 gactgtctga gcacttttgt actttgtcat ctgactaaaa aggcgccgaa gcgcctttag
    44881 aaaatagtcg aatcagtgaa ttactggtat tcgctaatcg gtacgcagga gcagaacagg
    44941 ttacggtcgc cgtaaacatc atccagacgt ttcaccgtcg gccagtattt gtctgccaca
    45001 cctgccggga atactgcaac ctcacggctg tacggatgcg cccactcggc gaccagttcg
    45061 ttctgaatgt gcggcgcgtt caccagcggg ttatcttcca gcggccagac accggcttta
    45121 acctggtcga tttctgcgcg gatagccagc atcgcgtcga taaagcgatc cagttccact
    45181 ttgctttcag attcagtcgg ttcaaccatc agcgtacccg ccaccgggaa cgacatggtc
    45241 ggcgcgtgga aaccgtagtc gatcaggcgc ttggcaatat ccagctcgct gatgccggtt
    45301 tcttctttca gcgggcgaat atcgagaata cattcgtgcg ccacgcgacc gtcgcgaccg
    45361 gtatacagca ccgggaaggc atcctgcagg cggctggcaa tatagttggc gttgaggatt
    45421 gccacctggc ttgctttttt cagcccttct gcgcccatca tgcggatgta catccagctg
    45481 attggcagga tagaggcgct accgaacggt gccgcagaaa ctgcgccctg acgggttaac
    45541 atgccttcga tttgcaccac gctatgaccc ggtacaaacg gtgccagatg cgctttcaca
    45601 ccgatcggtc ccatacccgg accaccaccg ccgtgcggaa tgcagaaagt tttatgcagg
    45661 ttgaggtgcg aaacgtccgc gccaataaag cccggcgagg tgatgccaac ctgggcgttc
    45721 atgttcgcgc catcaaggta aacctgaccg ccgaactgat gcacgacttc acacacttca
    45781 cggatcgttt cttcatacac gccgtgggta gaaggataag tcaccatgat acaggagagg
    45841 ttatcgcccg cctgttccgc tttcgcgcgc agatcagtca gatcgatgtt gccgttttta
    45901 tcacacgcca caaccaccac ctgcattcct gccatatgtg cagaagcggg gttagttccg
    45961 tgcgcagaag ccgggatcag gcagatatcg cgatgccctt cgttgcggct ttcatgataa
    46021 tgacgaatcg ccagcaggcc cgcgtattcg ccctgtgcgc cggagttcgg ctgcatacaa
    46081 acggcgtcgt aaccggtcag tttcaccagc cagtcagcca gctgcgcaat catctgctga
    46141 taaccttcag cctgttccgg cggacaaaac gggtgcagtt cggcaaattc cggccaggtg
    46201 attgggatca tctcggcggc ggcgttcagt ttcatggtgc aggaacccag cgggatcatc
    46261 gcctgattca gcgccagatc tttacgctcc agcgagtgca tatagcgcat catttcggtt
    46321 tcgctgtggt agcgattaaa caccggatgg gtgaggattt cgtcgtcgcg cagcatcgca
    46381 ggctggatag agcggctgtc gtgagccacg tctttgtcca gcgtgtcgat gtccaggccg
    46441 tggttatcgc ccagtagtac gctgaaaagc tgcattacgt tttcacgcgt ggttgtttca
    46501 tcaagggtga tcccaaccgc gttcagaata tcgctacgca ggttgatttc agccgcttcg
    46561 gcacgcgtca gtacgcccgc tttgtcggcc acttccacac acaaggtgtc gaaatagtgc
    46621 gcatggcgca gtttcagacc tttttgttgc aggcccgccg ccaggatatc ggtcagacgg
    46681 tgaatgcggt tagcgatacg tttcaggcca atcgggccgt gataaacggc atacaggctg
    46741 gcgatgtttg ccagcagcac ctgggaagta caaatgttgg agttcgcttt ctcacggcga
    46801 atatgttgct cgcgagtctg catcgccatg cgcagcgcgg tattgccagc tgcatctttc
    46861 gatacaccga taatacggcc cggcattgag cgtttgtatt catctttcgc cgcaaagaag
    46921 gccgcgtgtg ggccaccgta gcccatcggc acgccgaagc gttgcgccga accaaaaaca
    46981 atatccgcgc cctgtttacc cggcgcagtt aacagcacca gcgccataat atcggcggcg
    47041 acactgacca caattttgcg tgatttcagt tcgctaataa gcgcggtgta gtcgtggatt
    47101 tcaccggttg tgccaacctg ctgtaacagc acgccgaaga cgtcctgatg gtcgagcact
    47161 ttttgcgcgt catcgacaat cacttcaaaa ccaaaggttt cggcacgagt acggaccaca
    47221 tccagcgttt gcggatgcac atcggaagcc acgaagaagc ggttggcatt tttcagtttg
    47281 ctgacgcgtt tcgccatcgc cattgcttcg gcggcagcgg tggcctcgtc cagaagagaa
    47341 gcagaggcca tatccagtcc agtcaaatcc agcgttacct gctggaagtt gagcagtgct
    47401 tcaaggcggc cctgggagac ttcaggttga tacggagtgt acgcggtata ccagcccgga
    47461 ttttccagca tgttacgcag gataaccggc ggtagctgca cggcggtgta acccatgccg
    47521 atgtaagacg tgaagcgttt attgcgactg gcaatagcct tgagttctgc cagtgcggcg
    47581 tattcggtcg ccggtgcgcc aacctgcggc ggggtcgcaa gctgaatatc tttcggcaca
    47641 atctggccgg tcagcgcgtt taacgattgt gcaccaacgg cattcagcat ttcttgctgt
    47701 tgcgcggcgt ccggtccgat atggcgttca ataaaagcgc cgctgttttc aagctggctt
    47761 aacgtctgtg tcatgagcga tggttcctga aacgtgcagt gaattgtgaa cctctctcct
    47821 tacgaagaga gtgagggtga ggcataaatt ttcctcaccc tgatcctctc ccgcagaaga
    47881 ggaataaagc cgttactcgt cttctaacaa tgcttcgtat gcggtcgcat ccagcagtga
    47941 ttccagttcg ctttcatcgc tggctttgat tttaaagatc cagccacctg catacggttc
    48001 gctgttcacc agttccgggg aatcgctcag tgcgtcgttt accgccacga tttcaccgct
    48061 tactggcgca taaatgtctg acgccgcttt tactgattcg gcaaccgcgc agtcatcgcc
    48121 cgcgctaacc gttgcgccca cttccggcag gtcaacaaac accatatcgc ctaacagctc
    48181 ctgggcatgt tcggttatac caacggtgta agtgccgtcg gcttctttac gcagccattc
    48241 gtgttctttg ctgtatttca gttctgctgg tacgttgctc atcaatcaat ctccaaaaaa
    48301 gtaaatcacg cgacggcttt accgttacgc acaaaaacag gtttcgtcac tttaaccggc
    48361 atttcacggt tgcgaatttg cacaatcgcc gtttcgccaa taccttccgg cacgcgtgcc
    48421 agcgcaatgc tgtaacccag cgtcggggag aaagtcccgc tggtgataat gccttcatgc
    48481 tggttgccct gcgcatcggt aaaacgtacc ggcagttcat tacgcagcac gcctttttcg
    48541 gtcatcacca gaccaaccag tttttctgtg ccatgctcac gctgcacttc cagggcttca
    48601 cgaccgatga agtcacgatc tgccggttcc caggcgatag tccagcccat gttggcggct
    48661 aaaggagaaa tagtttcgtc catctcctga ccataaagat tcatgcccgc ttccagacgc
    48721 agcgtgtcac gcgcgcccag accgcatggc ttaacgcccg cttccaccag cgcacgccag
    48781 aaatcggccg ctttttcatt gggcagcgca atttcatagc ccgcttcacc ggtataacca
    48841 gtggtggcaa taaacagatc gcccgcctgc acgccaaaga acggtttcat cccttccacc
    48901 gcctgacgct gagcatcatt aaacagtgtg gcagcttttg cctgcgcatt cggcccttgt
    48961 acggcgatca tggaaaggtc atcacgaacg gtaatttcga tgccgaaagg ttcagcgtgt
    49021 tgggtaatcc aggagaggtc tttttcgcgg gtggcggagt taacaacgag gcggaagaaa
    49081 tcttcagtaa agtagtagac gatgaggtca tctatcacac cgccagaggc attcaacatc
    49141 cccgagtaaa gggctttgcc gcttttggtg agcttcgcca catcgttcgc cagcagataa
    49201 cgcagaaact cccgggtgcg gctgccgcga agatcgacga tggtcatatg tgacacatca
    49261 aacattccgg catcggtacg taccgcatga tgttcgtcga tttgcgaacc gtaatgcagc
    49321 ggcatcatcc agccgtggaa atccaccatg cgagcgccgc aaagcgtgtg ttgttcgtac
    49381 aaaggagtct gttgtgccat cttgtcctca ttgaataagc ggggctgaca actttttcat
    49441 ggtgaaatta tcaccacgaa acccagcatc ggagccactc ccggtcccca acgcaatcgt
    49501 tctcttttgc ctgaacttac caccgaaaca gactgttaac cataaggtaa aattgatcat
    49561 cacattagct tatggttaaa aaacgccaaa atcgcaacag aataaaaaac caaaaaatac
    49621 acaagttttt atataaagat gatgtgatga gaaagttaat ttgaataagg caatattaag
    49681 agctgaaaaa atgtcaaaaa acactaaatc aaaaattcgt gacattagaa aatataatgc
    49741 gaaaacggag gtgaaattag tttatttcaa atgaggaaaa tctcccggcg aaaaaaccgg
    49801 gagatgaaag tgtgatgggt atcaaataaa cagcagaaga gaaattttta acgcagccat
    49861 tcaggcaaat cgtttaatcc cattgcctgg cggataagtt gtggtttaac gccaggaagc
    49921 gtgtcggcca gtttcaaacc aatatcacgc agcagttttt tcgccggatt agtaccggaa
    49981 aacagatcgc ggaatccctg cataccagcc agcatcagcg ccgcactgtg cttgcggcta
    50041 cgctcatacc ggcgcaggta aatgtactgc ccgatgtctt tcccctgacg atgcaaccgt
    50101 ttcagttcag caatcagctc tgcagcatcc ataaagccga gatttactcc ctgccccgcc
    50161 agcgggtgaa tggtatgcgc ggcgtcgccc accagcacca gacggtgcgc ggcaaactgg
    50221 cgcgcataac gccccgtcag tgggaacacc tgacgcgcgc tctcaacctt gcataagccc
    50281 aggcgattat caaaagcgat atttaacgcg cgattaaatt catcttcact tgcctgctgc
    50341 atccgctgcg cttcctctgg cgacagtgac cagacaattg agcaaagatg cggatcgcta
    50401 agcggtaaaa aggccagaat gccttcgcca tggaaaacct gccgcgccac cgcatcatgc
    50461 ggttcttccg tgcgaatggt cgctaccagc gcgtgatgct gataatccca gaaagtcagc
    50521 ggaatatctg ctttgttgcg caaccaggaa ttagcaccgt ccgcgccaat caccagacgc
    50581 gccgttaaca tgctgccatc tttcagcgtc aggaaagttt cattttctcc ccaggcgacc
    50641 tgctgtaatt ctgcgggggc caacagagtg atatctgacg actgctgcgc tttgttccac
    50701 agcgcgtagt gaatcactga gttttcaacg atatgcccaa gatggctata gcccatgctt
    50761 tgatcgtcaa acgaaatgtg accaaaactg tctttgtccc acacttccat accgtgataa
    50821 caactggccc tacgagagag aatgtcctgc cagacgccaa gacgggtgag taatttttcg
    50881 ctggcggcat tgatagccga aacgcgcagt tgtggtggtg catccgccgc cagaggttcc
    50941 ggtacgcgct gctccagtac ggcaacgcgt aagccgctcc cctgtaagcc gcaggcaacc
    51001 gccagcccca ccatgccgcc accaacaatg gctacatcaa cactttgcat tgtttattcc
    51061 ttaaaaccgc cttcaacgcg ccacccaacc gagggtgcgc tgcgccagca catcgcgtgc
    51121 cggggtgaat aattccatcg tcatcagtcc gatgttgcgc ccgacaacca gcggtgccca
    51181 acggttggca aaaagatgta ccaggctgtc cgtgacgccg atggttgctt cgcgatcact
    51241 ctgtcgacgc tgctgatagc ggcacaatat gccgtaatca ccgatgtctt ctccgcgctc
    51301 atgcgcctga gtcagggttt ccgcaaggct catgacatct cgcataccga ggttaaaccc
    51361 ttgcccggca atcgggtgca gagtttgcgc cgcattgccc accagcacgg tacgatgggt
    51421 aatagctctg gcggcgcggg ttaacgccag cggataagca ctgcgtttac cggcgtgggt
    51481 aatttttcca agtcgccagc caaaggccga ctggagttca cggcaaaact tctcgtcgct
    51541 ccacgacagc acctcttcgc gccgttccag tggatgacac cagaccagcg aacagcgtcc
    51601 gtcagacatc ggcaacatcg ccagcgggcc atgttgcgtg aagcgttcaa aagcgcggcc
    51661 ttcgtgcgca acggaagtcg caacgttggc aatcacggcc agttgttcgt aaggctcctg
    51721 ctgccagtca acgccgcacg cggtggctaa cgctgaatgg gtgccatcag ctgctaccag
    51781 cacgcggccc gtcagcgtct cgccactctc cagcgtcact tcaacgtgac tctgagtacg
    51841 ggcaacgtta gccacgcgat cagggcaatg cagcgttacg ccaggtgctt tacgcagcaa
    51901 tgcaaacagc cgttgcccga cattgtgcaa ttcgacaacc tgtcccagcg ccgccagttg
    51961 gtaatcttct gcggcgaggg tgacaaatcc agcgtgacca cgatcgctga catgcacggt
    52021 agtgatggcg gttgcgtaat ccgccagaga ttgccagacg ccgatgcgcg ccagttgctg
    52081 acaggtcccc gcagccagcg caatagctcg tccatcaaag cccggatgag catgtgactc
    52141 tggcgcggtc gcttcaatca aatgtaccgg cagcgccccg tgacttaacc gggaaatagc
    52201 cagcgccagc gtcgcgcccg ccatgccgcc accgacgatg attacgctca ttgctttctc
    52261 gcagctgcca tcaacgcttc gatttcttcc ggctttttca ccacgctggc ggtgaggttt
    52321 tcgttgccgg tttcggtaat cacaatgtcg tcttcaatac gaatgccgat accgcgatat
    52381 tgttctggca catccgcatc cggcgcgata tacagaccag gctctacggt cagtaccatc
    52441 cccggttcca gaatgcgcga gcgatcctga ccataaacgc caacgtcatg gacatctagc
    52501 cctaaccagt ggctaaggcc atgcataaag aaaggacgat gggcgttctg agcgatcagt
    52561 tcatcaacat cacctttcag aatgccgagt tttaccaggc cgctaaccat gatgcgcacc
    52621 acttcaccag tgacttccag aatggaagtt cccggacgat acaggcgcaa gctggtttcg
    52681 agagactcca gcacaatgtc gtagatttca cgctgggcct gagtgaattt gccgttgacc
    52741 gggaaggtgc gggtaatatc gccagcgtaa cctttgtatt cgcaacccgc gtcaatcaac
    52801 accaggtcgc cgtcgcgcat ttcacactcg ttttcggtgt agtgcagaat gcagccgttt
    52861 tcaccgctgc cgacaatggt gttataggac ggatagcgcg caccgtggcg gttaaattcg
    52921 tggtgaattt cgccttccag atggtactcg aacattcccg gacggcattt ttccatcgcc
    52981 cgggtatgtg ccatggcggt gatttctccc gcgcggcgga gtacggcaat ctcttctggc
    53041 gatttgaaca ggcgcatttc atgaacaaca ggacgccagt caatcatcgt tgccggtgcg
    53101 gtgagatttt gccgcgaacc tttacgcagt ttttccagcg cactgttcac gatttcatca
    53161 gcatatgcat attcgccctg ggcatggtaa accacatcca ggccgttaag tagttgataa
    53221 agttgctgat tgatttcgct gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg
    53281 gcatcctggc ctaagcgacg gccaaaccag atctccgccg tcaggtcgcg aacgcggtta
    53341 aacagaacgc tgtggttatg agtgtcatcg cttttaatca gcaccagcac cgcttccggt
    53401 tcgttaaagc cggtgaagta ccagaagtca ctgttctgac gataggggta ttcgctgtcg
    53461 gcgctacgtg ttacttctgg tgcagcaaaa atcagcgcgg cgctgccggg ttgcatttgc
    53521 tccaccaggg cctgacggcg acgctgaaac tcttgccggg atatctcact cataacactc
    53581 tccttacgtt ttttgttttt agtgtagagt cggtttttgt acttctggcg cggtcggttg
    53641 cggatgagta aaggtgtcgt ggcataacag cgcggcaaca cgaacgtatt cgatgatctc
    53701 ttcaagcgac atttcaagct cttcctgatc ttcgtcttcg tcgtaaccca gttgcgcaat
    53761 gttacgcaga tcgtcgatag cttcaccggt ttcgccggtc actttatcca gcttcggttg
    53821 cgtaacgcca agaccaagca ggaagtgatt gacccaacct gccaatgcat cagcccgatc
    53881 gaaaacgctg acatcatcgc catcaggcag ataaagctga aaaaggaagc cgtcatcctg
    53941 cagggcatcg ctggtggcag agtgcatttt acgcagtgcc tgtgccagct catgaccgaa
    54001 agccatgcct tcgttcgtca ggtcgtgaag tagcggtagc catgagctgt catcgttacc
    54061 gccacatatc atcccactga ttaaaccatg catctcagca ggggtcagac ccgtcccttg
    54121 ttggttcaga tactggttca tttcgttgta accaggcatt tcgttctgta tagacataag
    54181 cattcgtcgt caaagggagg aatattcatg atatgctacc actttgggcc ctggtggacc
    54241 agaaaagggc ttgtctcttc tcatcagggt agctatagtg tcgccccttc gcagaccatg
    54301 ggtctaaaga cgaaggcagc gcagtcaatc agcaggaagg tggcatgtct gcacaacccg
    54361 tcgatatcca aatttttggc cgttcactgc gtgtgaactg cccgcctgac caaagggatg
    54421 cgttgaatca ggcagcggac gatctgaacc aacggttgca agatctgaaa gaacgcacta
    54481 gagtcacaaa tactgaacag ttggtcttca ttgccgcatt gaatatcagc tatgagttag
    54541 cgcaagaaaa agcgaagact cgtgactacg cggcaagtat ggaacagcgt attcggatgc
    54601 tgcagcagac catagaacaa gcgttacttg aacaaggtcg catcaccgaa aaaactaacc
    54661 aaaactttga atgacacttt tcggtttact gtggtagagt aaccgtgaag acaaaatttc
    54721 tctgagatgt tcgcaagcgg gccagtcccc tgagccgata tttcatacca caagaatgtg
    54781 gcgctccgcg gttggtgagc atgctcggtc cgtccgagaa gccttaaaac tgcgacgaca
    54841 cattcacctt gaaccaaggg ttcaagggtt acagcctgcg gcggcatctc ggagattccc
    54901 ttcttatctg gcaccagcca tgacgcaact accagaactc ccactgacat tatcccgaca
    54961 agaaatccgc aaaatgattc ggcaacgtcg tcgtgcgtta acgccggaac aacagcagga
    55021 aatgggtcaa caagccgcta cccggatgat gacttatccc ccggtggtga tggcgcatac
    55081 ggtcgctgta ttcctctctt ttgatggtga actcgacacc cagccgctaa tagaacaact
    55141 ctggcgcgcc ggtaagcgcg tatatcttcc agttttgcat ccctttagtg ccggtaattt
    55201 gctgttcctg aattaccatc cgcaaagcga actggtgatg aacaggttga agatccatga
    55261 gccaaaactg gatgtgcgtg acgtgctacc cctttcccga ttagacgtgc tgatcacacc
    55321 gctggtcgcc tttgatgagt acggtcagcg cctgggaatg ggcggtggtt tttatgatcg
    55381 gaccttacaa aactggcagc actataaaac gcaaccggtg ggttatgcgc atgattgtca
    55441 gttggtggaa aaactccccg ttgaagagtg ggatatccct cttcctgcgg tggttacacc
    55501 gtcgaaagtc tgggagtggt aagggcgata cacctgcatc gccctgattg acatcgttga
    55561 ttctttaacc taatttagtg agtaagggta agggaggatt gctcctcccc tgtgactgac
    55621 tgttaataag ctgggaaact tatgagtaac agtacaatca gtatgatggc aagtcgcatc
    55681 ataacccttc tccttcaagc cctcgcttcg gtgagggctt taccgttaca gccccatgct
    55741 gcccagctat cgtaagctct ctttaaataa ccacaacgca ttgatctacc tttaaatctt
    55801 ttcttcgagc gtgctcgaaa aaccccatgc aaaacagaga taatttttgg gaaaaaggat
    55861 ctattacggc agtaaacgtg ggcgatgaag tggctatcaa aaagttcggt tcagggcgat
    55921 gcaccccgca tcgccctgat tgacatcgtt gattctttga cctaatttag tgagtaaggg
    55981 taagggagga tgactcctcc cctgagactg actgttaata agctccgaaa cttatcagta
    56041 acagtacaat cagtatgatg acaagtcgca tcataaccct tctcctttaa gccctcgctt
    56101 cggtgagggc tttaccgtta cagccccatg ctgtcccgcc atcgtaaatc cccattaaat
    56161 aaacacaacg cattgatctg actttgattt attttctgga gcagactcgc aaagtagaat
    56221 gcgcaacgcg gcaacggtgt ggagaaggga taaaaaaacg ggcaagtcag tgacctgccc
    56281 gttaattttc agagaagggg aattagtaca gcagacgggc gcgaatggta cccggaatag
    56341 ctttcattgc ctgcagcgct ttttcggcaa cgtcttcgtc agcttcaata tcaataacta
    56401 cataacccat ctgggcggaa gtttgtagat attgcgcggc gatgttgacg ccctgctcgg
    56461 cgaagatttt gttcagcgca gttagcacgc ccggacggtt ttcgtggatg tgcatcagac
    56521 gacgaccacc atgcagtggc agcgagactt ccgggaagtt caccgcagag agcgttgagc
    56581 cattgtcaga atacttgatc aatttacccg caacttccag gccgatattc tcctgcgctt
    56641 cctgagtcga accgccaatg tgtggcgtca gaaggacgtt gtcgaattca cacagtggag
    56701 aggtaaatgg atcgctattg gtcgccggtt ccgtcgggaa tacgtcgatt gccgcccccg
    56761 ccagatgttt gctcgccagc gcatcacaca gcgccggaat atccaccaca gtaccgcgcg
    56821 aggcattaat cagcagcgag ccgggcttca ttagcgaaat ctctttcgcg cccatcatat
    56881 ttttggtgga cggattctct ggtacatgca gactcaccac atcgctcata ttcagcaggt
    56941 cagaaagatg ctgtacctga gtggcgttgc ccagcggcag tttgttttca atatcataaa
    57001 agtaaacata cattcccagc gattcagcca gaatgcccaa ttgcgtacca atatgaccgt
    57061 agccgatgat acccagcttt ttgccgcgcg cttcaaaaga acccgccgcc agtttgttcc
    57121 acacgccacg gtgcgcttta gcattggctt ccggcacgcc gcgcaatagc agcagcagtt
    57181 cgccaatcac cagctccgca acagagcgcg tatttgagaa cggtgcgtta aataccggga
    57241 tcccgcgctt tgccgccgca tccagatcaa cctggtttgt tccgatacag aaacagccaa
    57301 tagcgaccag tttttctgcg gcgttaatca cgtcttcagt cagatgggta cgggatcgca
    57361 ggccgatgaa gtgggcatcg cggatggatt cttttaattg ttcatcatcc agcgcgcctt
    57421 tgtgaaattc gatgttggtg taaccagctg cacgaaggct ttccagcgcc ttttggtgca
    57481 cgccttctac cagcagaaac ttaatcttgt ctttctccag cgataccttt gccatttacc
    57541 caatcctgtc ttttgaaatg ttgtgtgcgg atttgcatcc gcctttcaac atatcaaaaa
    57601 ataatattgc ggcaatatga acgtttgcgt cgcgatgttg aggaaatatc acgcaaaggt
    57661 aattcttgga tgaatatgct ggtttagagg atttatttag agcaatcgac aattgcctgg
    57721 taaaagcgtg acacatgtca ccaaatttaa tgaagagaat ttttttaacg ggggaggttc
    57781 ccccgtcaga tcatttcaca atggttttga caccgtcagg tgtgccaatc agcgcaacgt
    57841 ccgcgccacg gttagcaaac aagccaacag tcaccacgcc aggaatcgca tttatggcgt
    57901 tttccatcgc tatcgggtca aggatttcca tgccgtggac gtcgaggatc acgttgccat
    57961 tatcggtcac cacgccctga cggtattccg gacgaccgcc cagtttcacc agctgacgcg
    58021 ccactgcact acgtgccatc gggataactt ctactggcag cgggaattta cccagaatat
    58081 caacctgctt ggaagcgtct gcaatacaga taaatttttc tgcaaccgaa gcaatgattt
    58141 tttcacgggt cagcgccgcg ccgccgcctt tgatcatttg catgtggccg ttgatttcat
    58201 ctgcgccatc aacgtagatg ccaaggctgt cgacttcgtt gagatcaaaa acgtgaatgc
    58261 cgaggctttt cagtttttca gtggaagcat ctgaactgga aacggcccct tcaatctggc
    58321 ctttcattgt accgagcgcg tcaataaagt gtgcggcggt ggaacctgta cctacaccaa
    58381 caatggtgcc gggctgaaca tactgaagtg ccgcccatcc tactgctttt ttcaattcat
    58441 cctgcgtcat gatcgtttcg cctgtggtat gaaatttcac acgcattata tacaaaaaaa
    58501 gcgattcaga ccccgttggc aagccgcgtg gttaactcat ccataaaata tcgcgcaatg
    58561 gcgggcatcc cctttcgccc cgcaaataaa gcatacaacg gtctgggtat gccgctccac
    58621 ggtgcgaaca gaggcaataa cgccacgcca ttcaacacaa ccagggcgcg aactcgctga
    58681 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acgaatacaa
    58741 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    58801 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    58861 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    58921 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    58981 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    59041 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    59101 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttcctgcac
    59161 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    59221 gcactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    59281 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    59341 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcgc
    59401 cagtggtcgc gtttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    59461 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtacaac
    59521 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagagctgg
    59581 ccagcggtga actgattgac ttaacgcctg ggctatttca acgacggatg ctctactggc
    59641 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgtta ctcgattatg
    59701 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    59761 gttgtcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    59821 ggcaaaaaac gacgtctgaa tgcatttttt ttgctggcga caaacccacg taaaaagctc
    59881 accgtaggcg caaataccct cattttgatt gcgttttacg gagcaaataa tgtctaacgt
    59941 gcaggagtgg caacagcttg ccaacaagga attgagccgt cgggagaaaa ctgtcgactc
    60001 gctggttcag caaaccgcgg aagggatcgc catcaagccg ctgtataccg aagccgatct
    60061 cgataatctg gaggtgacag gtacccttcc tggtttgccg ccctacgttc gtggcccgcg
    60121 tgccactatg tataccgccc aaccgtggac catccgtcag tatgctggtt tttcaacagc
    60181 aaaagagtcc aacgcttttt atcgccgtaa cctggccgcc gggcaaaaag gtctttccgt
    60241 tgcgtttgac cttgccaccc accgtggcta cgactccgat aacccgcgcg tggcgggcga
    60301 cgtcggcaaa gcgggcgtcg ctatcgacac cgtggaagat atgaaagtcc tgttcgacca
    60361 gatcccgctg gataaaatgt cggtttcgat gaccatgaat ggcgcagtgc taccagtact
    60421 ggcgttttat atcgtcgccg cagaagagca aggtgttaca cctgataaac tgaccggcac
    60481 cattcagaac gatatcctca aagagtacct ctgccgcaac acctatattt acccaccaaa
    60541 accgtcaatg cgcattatcg ccgacatcat cgcctggtgt tccggcaaca tgccgcgatt
    60601 taataccatc agtatcagcg gttaccacat gggggaagcg ggtgccaact gcgtgcagca
    60661 ggtagcattt acgctcgctg atgggattga gtacatcaaa gcagcaatct ctgccggact
    60721 gaaaattgat gacttcgctc ctcgcctgtc gttcttcttc ggtatcggca tggatctgtt
    60781 tatgaacgtc gccatgttgc gtgcggcacg ttatttatgg agcgaagcgg tcagtggatt
    60841 tggcgcacag gatccgaaat cactggcgct gcgtacccac tgccagacct caggctggag
    60901 cctgactgaa caggatccgt ataacaacgt tatccgcacc accattgaag cactggctgc
    60961 gacgctgggc ggtactcagt cactgcatac caacgccttt gacgaagcgc ttggtttgcc
    61021 taccgatttc tcagcacgca ttgcccgcaa cacccagatc atcatccagg aagaatcaga
    61081 actctgccgc accgtcgatc cactggccgg atcctattac attgagtcgc tgaccgatca
    61141 aatcgtcaaa caagccagag ctattatcca acagatcgac gaagccggtg gcatggcgaa
    61201 agcgatcgaa gcaggtctgc caaaacgaat gatcgaagag gcctcagcgc gcgaacagtc
    61261 gctgatcgac cagggcaagc gtgtcatcgt tggtgtcaac aagtacaaac tggatcacga
    61321 agacgaaacc gatgtacttg agatcgacaa cgtgatggtg cgtaacgagc aaattgcttc
    61381 gctggaacgc attcgcgcca cccgtgatga tgccgccgta accgccgcgt tgaacgccct
    61441 gactcacgcc gcacagcata acgaaaacct gctggctgcc gctgttaatg ccgctcgcgt
    61501 tcgcgccacc ctgggtgaaa tttccgatgc gctggaagtc gctttcgacc gttatctggt
    61561 gccaagccag tgtgttaccg gcgtgattgc gcaaagctat catcagtctg agaaatcggc
    61621 ctccgagttc gatgccattg ttgcgcaaac ggagcagttc cttgccgaca atggtcgtcg
    61681 cccgcgcatt ctgatcgcta agatgggcca ggatggacac gatcgcggcg cgaaagtgat
    61741 cgccagcgcc tattccgatc tcggtttcga cgtagattta agcccgatgt tctctacacc
    61801 tgaagagatc gcccgcctag ccgtagaaaa cgacgttcac gtagtgggcg catcctcact
    61861 ggctgccggt cataaaacgc tgatcccgga actggtcgaa gcgctgaaaa aatggggacg
    61921 cgaagatatc tgcgtggtcg cgggtggcgt cattccgccg caggattacg ccttcctgca
    61981 agagcgcggc gtggcggcga tttatggtcc aggtacacct atgctcgaca gtgtgcgcga
    62041 cgtactgaat ctgataagcc agcatcatga ttaatgaagc cacgctggca gaaagtattc
    62101 gccgcttacg tcagggtgag cgtgccacac tcgcccaggc catgacgctg gtggaaagcc
    62161 gtcacccgcg tcatcaggca ctaagtacgc agctgcttga tgccattatg ccgtactgcg
    62221 gtaacaccct gcgactgggc gttaccggca cccccggcgc ggggaaaagt acctttcttg
    62281 aggcctttgg catgttgttg attcgagagg gattaaaggt cgcggttatt gcggtcgatc
    62341 ccagcagccc ggtcactggc ggtagcattc tcggggataa aacccgcatg aatgacctgg
    62401 cgcgtgccga agcggcgttt attcgcccgg taccatcctc cggtcatctg ggcggtgcca
    62461 gtcagcgagc gcgggaatta atgctgttat gcgaagcagc gggttatgac gtagtgattg
    62521 tcgaaacggt tggcgtcggg cagtcggaaa cagaagtcgc ccgcatggtg gactgtttta
    62581 tctcgttgca aattgccggt ggcggcgatg atctgcaggg cattaaaaaa gggctgatgg
    62641 aagtggctga tctgatcgtt atcaacaaag acgatggcga taaccatacc aatgtcgcca
    62701 ttgcccggca tatgtacgag agtgccctgc atattctgcg acgtaaatac gacgaatggc
    62761 agccacgggt tctgacttgt agcgcactgg aaaaacgtgg aatcgatgag atctggcacg
    62821 ccatcatcga cttcaaaacc gcgctaactg ccagtggtcg tttacaacaa gtgcggcaac
    62881 aacaatcggt ggaatggctg cgtaagcaga ccgaagaaga agtactgaat cacctgttcg
    62941 cgaatgaaga tttcgatcgc tattaccgcc agacgctttt agcggtcaaa aacaatacgc
    63001 tctcaccgcg caccggcctg cggcagctca gtgaatttat ccagacgcaa tattttgatt
    63061 aaaggaattt ttatgtctta tcagtatgtt aacgttgtca ctatcaacaa agtggcggtc
    63121 attgagttta actatggccg caaacttaat gccttaagta aagtctttat tgatgatctt
    63181 atgcaggcat taagcgatct caaccggccg gaaattcgct gtatcatttt gcgcgcgccg
    63241 agtggatcca aagtcttctc cgcaggccac gatattcacg aactgccgtc tggcggtcgc
    63301 gatccgctct cctatgatga tccattgcgt caaatcaccc gcatgatcca aaaattcccg
    63361 aaaccgatca tttcgatggt ggaaggtagt gtttggggtg gcgcatttga aatgatcatg
    63421 agttccgatc tgattatcgc cgccagtacc tcaaccttct caatgacgcc tgtaaacctc
    63481 ggcgtcccgt ataacctggt cggcattcac aacctgaccc gcgacgcggg cttccacatt
    63541 gtcaaagaac tgatttttac cgcttcgcca atcaccgccc agcgcgcgtt ggctgtcggc
    63601 atcctcaacc atgttgtgga agtggaagaa ctggaagatt tcaccttaca aatggcgcac
    63661 cacatctctg agaaagcgcc gttagccatt gctgttatca aagaagagct gcgtgtactg
    63721 ggcgaagcgc acaccatgaa ctccgatgaa tttgaacgta ttcaggggat gcgacgcgcg
    63781 gtttatgaca gcgaagatta ccaggaagga atgaacgctt tcctcgaaaa acgtaaacct
    63841 aatttcgttg gtcattaatc cctgcgaacg aaggagtaaa aatggaaact cagtggacaa
    63901 ggatgaccgc cgatgaagcg gcagaaatta tccagcataa cgacatggtg gcatttagcg
    63961 gctttacccc ggcgggttcg ccgaaagccc tacccaccgc gattgcccgc agagctaacg
    64021 aacagcatga ggccaaaaag ccgtatcaaa ttcgcctgct gacgggggcg tcaatcagcg
    64081 ccgccgctga cgatgtactt tctgacgccg atgctgtttc ctggcgtgcg tcatatcaaa
    64141 catcgtccgg tttacgtaaa aagatcaatc agggcgcggt gagtttcgtt gacctgcatt
    64201 tgagcgaagt ggcgcaaatg gtcaattacg gtttcttcgg cgacattgat gttgccgtca
    64261 ttgaagcatc ggcactggca ccggatggtc gagtctggtt aaccagcggg atcggtaatg
    64321 cgccgacctg gctgctgcga gcgaagaaag tgatcattga acttaatcac tatcacgatc
    64381 cgcgcgttgc agaactggca gatattgtga ttcctggcgc gccaccgcgg cgcaatagcg
    64441 tgtcgatctt ccatgcaatg gatcgcgtcg gtacccgcta tgtgcaaatc gatccgaaaa
    64501 agattgtcgc cgtcgtggaa accaacttgc ccgacgccgg taatatgctg gataagcaaa
    64561 atcccatgtg ccagcagatt gccgataacg tggtcacgtt cttattgcag gaaatggcgc
    64621 atgggcgtat tccgccggaa tttctgccgc tgcaaagtgg cgtgggcaat atcaataatg
    64681 cggtaatggc gcgtctgggg gaaaacccgg aaattcctcc gttcatgatg tattcggaag
    64741 tgctacagga atcggtggtg catttactgg aaaccggcaa aatcagcggg gccagcgcct
    64801 ccagcctgac aatctcggcc gattccctgc gcaagattta cgacaatatg gattactttg
    64861 ccagccgcat tgtgttgcgt ccgcaggaga tttccaataa cccggaaatc atccgtcgtc
    64921 tgggcgtcat cgctctgaac gtcggcctgg agtttgatat ttacgggcat gccaactcaa
    64981 cgcacgtagc cggggtcgat ctgatgaacg gcatcggcgg cagcggtgat tttgaacgca
    65041 acgcgtatct gtcgatcttt atggctccgt cgattgctaa agaaggcaag atctcaaccg
    65101 tcgtgccaat gtgcagccat gttgatcaca gcgaacacag cgtcaaagtg atcatcaccg
    65161 aacaagggat cgccgatctg cgcggtcttt ccccgcttca acgcgcccgc actatcattg
    65221 ataattgtgc acatcctatg tatcgggatt atctgcatcg ctatctggaa aatgcgcccg
    65281 gcggacatat tcaccacgat cttagccacg tcttcgactt acaccgtaat ttaattgcaa
    65341 ccggctcgat gctgggttaa tggtttgtcc cgtcacgatc ttcagcaata tgctgaagca
    65401 tcgtggcggt ataacgatga tttctgattg aataaagata ttcctgcata tacatcggcc
    65461 tgtgcggcgc atcaaaatat ttcagcgttg ccggattcac cagtcgccag gcaaaatgcg
    65521 ggatcaccgt cagaaaatgt ccgcgctcca cggcgctgat tttggccata aaactgtagg
    65581 gacgataaat aatggtggga ttgatcccac aaggtcgcat attcgcatca atcattgctt
    65641 cgaagttagc ccggttttga aagcgcattt gtagccaggg cagctcctga agcagctctt
    65701 gctcttgacg ctcttcataa cggcgagaaa cgagaaagcc caatcgcagc ggtggtaatt
    65761 cactgatagt caggttttcc agttcctgaa ctcgtgcaga tacatgctgt ggggagataa
    65821 taaaatctaa ctgacggtca aaaagattat cgataacacc gttttcgctg aactcaactg
    65881 gctgggcggt cacgccttca tacttatctc cgagactaat caactgatcg aaaataattg
    65941 tcggataagt attatctatg ccaatcacaa tttctcgtga acgacgccct gagttatgga
    66001 tttcattatc aatcgcagaa agtcgttgat aaaccgggaa cagtttttga tacagttcct
    66061 gaccggcttt attgaggctg atattattat ctttgcgggt aaatagcgta tagccaattt
    66121 gttcttccag cgcggcaatg cttttaccaa atggcgaagc cgtcatatgg attttttctg
    66181 ccgcccgggc aatattattg gtttgcgcta ataaaatgaa attgcgcatc tttttcgaaa
    66241 taaaaatgtc catcccccct ccccgcatcc ttgtccgctt aaccgtttca gtcagcctat
    66301 ccttgatgaa accgcgagca aagataggtg attacgtcat ggttttacag aaaattacag
    66361 aaaaaggagg caatatcggg taaaggcatt agcccgacga atacgtcggg ctacaagtat
    66421 tattgtgctg caggtgtttt agcgggttgt tgatccacag gttctaactg gaagaccaca
    66481 tcgacctgat catcaaactg aatagcggcc tgctcgtaag tttcctgggc agacaccggc
    66541 gcggcatcgg ctttcatcat ccgcaccatt gggctgggct gatagttgga aacatggtag
    66601 cgcacgctat ataccggccc cagtttacga tgaaagccgt tcgccagttc ctgcgcctga
    66661 tgaatcgcgt tatcaatcgc tgccttacgc gctttgtctt tataggcatc cggctgcgcc
    66721 acgcccagcg acacagaacg aatttcgtta agacccgcct tcagcgcgcc atccagcaag
    66781 gaattcagtt tgtctaactg acggagcgtg acttccaccg ttctcacagc gcggtagcct
    66841 ttcaggatac ttttaccatc ctgataatca taatctggct gggtgcgtaa attcgctgag
    66901 ctgatatctt ttttcgcgat ctgattgagt tcaaggaagg aaatgtattg tgcgacgcgc
    66961 tcatctgcct gtttcttggc tgtagcggca tccttcgcgg ccacgttaac ttcaatcgca
    67021 agagtggcaa tgtctggcac cgcatccaca cttgccgtac cggaggtgac aatatgcggt
    67081 ccatccggca attcgttagc ctgcgctgcc atcccgctaa tacccattaa tgccgccagg
    67141 gcgataactt tgaacttcac gttagtctct ccgtgttaat tgacttgccc aaaattcagg
    67201 acctctggag gcaagcttag cgcctctgtt ttatttttcc atcagatagc gcctaactga
    67261 acaaggcttg tgcatgagca ataccgtctc tcgccagctg caaggctata aaccacatga
    67321 cacatcccac taccagattg ataatgcgct gtgattttgc cgtgcgcagg cgcggtgcca
    67381 gccaggctgc gagaatagcc agaccaaaga accacaggaa agaggcgcta attgtcccga
    67441 gcgcaaacca acgttttggt tcgacgtcaa gctgcccgcc aaggctgccc agtacaacaa
    67501 aagtatccag gtaaacatgc ggattcagcc aggtcactgc caacatggtg gcgataattt
    67561 tccatctgcc ttgcttcagg acttcggcac tagctaactc aatattacta ctcatcgctg
    67621 ttttaaaagc gccaaaacca taccacagca agaagactac gccgccccag gtgaccagcg
    67681 ccagcaacca cggcgactgc atcaataacg cgctaccacc aaaaatcccc gcgcaaatca
    67741 ggaccaaatc gctgatagcg caaagtaagg caatcataat gtggtactga cgacgaatgc
    67801 cctgattcat cacaaaagca ttttgtgggc cgagcggcag aatcatagct gccccaagag
    67861 caagaccttg aaagtaataa gaaaacacgt tggatattcc gaattaagta tctgatgcag
    67921 agactatatc gcgggattgt tattagcgga aattgataat attaattggc aatcagaata
    67981 actaataaga gagaggccag agagtattat ctggcctcag cgttgattac gcagctttgt
    68041 cttctttcac ccgcttaaag ttcacatcca tttgcgggta cgggaagctg ataccggcgg
    68101 catcaaattc acgtttaata cgctccagca catcccagta cacgttttgc agatcgccgc
    68161 tgttgctcca gacgcggacc acgaaattaa tcgacgatgc accaagttcg ttcaggcgca
    68221 cagtcatttc gcgatctttc aaaatgcgat cttcagactg gataatattg gtcaggatct
    68281 gcttaacctg atcgatatcg gaatcatacg ccacgccaat aataaattcg ttacggcgaa
    68341 ctggctcgcg ggagaagtta ataatatttc cggcaataat tttaccgttc ggaataacga
    68401 taattttacc gtctgcagta cgcatggtgg tggagaaaat ctgcacactc agcacagtac
    68461 cggctacgcc gccgaggtca acatattctc cggcacggaa cgggcggaac atgacaagta
    68521 acacgccagc ggccaggtta gaaagtgacc cctgcaaagc cagaccaaca gctaagcctg
    68581 cggcaccgag tacagcaatg actgacgctg tttgtacacc cacgcgtccc agggctgcga
    68641 ttagcgtaaa ggcgataata ccgtaacgga ctaatgcaga aagaaaatca gcaacagtgg
    68701 catcgatttt acgggagatc atcaggcgat tcaccgcgtt ggaaatcatc cgcgcgataa
    68761 tcaaaccaac gatgatgatc gcgagtgccg ccacgatgtt tactgcataa cttagcagca
    68821 gcgcctggtt agctaccagc cagcttcccg cgccgtttat gctatcgaca acattcaaat
    68881 cttccattca atattccttt tcaactgact caaaatggag aaatacactc cgccgcctta
    68941 tgacgggcag tctgacaagg gtaaacaata aagcataatt ttgccaaata gatcacagat
    69001 ttctcatctg aatcaccttg cttttggcga aaaaatcccc gcagagcggg gctcagacag
    69061 atatcaatgt gcgctttgtc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
    69121 acctgcaaat tcaatatatt gcaggatacg tgtagatcct gataagcgta gcgcatcagg
    69181 caattttgct tttggcgaaa aaaagacccg cagagcgggc cttgagataa gcagaaagga
    69241 atgacttaca gaacgtcgat cgcgttcagt tcctggaatg ctttttccag acgagcgatc
    69301 atcgaagtct gaccggcacg cagccatacg cgcggatcgt agtatttctt gttcggctga
    69361 tcttcgcctt tcgggttacc cagctgaccc tgcagataag cttcgttcgc tttgtagtag
    69421 ttcagaacgc cttcccaggt tgcccattgg gtatcggtat cgatgttcat tttcactacg
    69481 ccgtagctta cggagtcttt gatttcctga gcagtagaac cggaaccacc gtggaatacg
    69541 aagttcaggc tgttgtgcgg caggttgtgt ttcttagaaa catattcctg agaatcacgc
    69601 aggatggtcg gagtcagaac cacgttaccc ggcttgtaaa caccgtgtac gttaccgaag
    69661 gacgcagcga tggtgaaacg cgggctgatt ttgctcagct cggtgtatgc gtaatcaacg
    69721 tcttccggct gggtgtacag tgcagaagcg tccatgtggc tgttgtccac gccgtcttct
    69781 tcaccaccgg tgcaacccag ttcgatttcc agagtcatgc cgattttgga catacgctcc
    69841 aggtatttag agcagatttc gatgttctct tgcagagatt cttcagacag gtcgatcatg
    69901 tgagaagaga acagcggctt accggtagct gcgaagtgtt tttcacccgc gtccagcaga
    69961 ccgtcgatcc acggcagcag tttcttcgcg cagtggtcag tgtgcaggat aaccggaaca
    70021 ccataatgtt cagccatctg gtgaacgtga tgcgcaccag agatcgcgcc caggatagca
    70081 gcaccctgcg gaacgtcaga tttcacgcct ttaccagcga taaaggaagc accaccgttg
    70141 gagaactgaa cgataaccgg cgctttaact ttagcagcag tttccagtac ggcgttgatg
    70201 gagtcagtac cgacgcagtt tactgctggc agtgcgaagt tgttttcttt tgctacctgg
    70261 aaaactttct gtacgtcatc accagtgatt acgccaggtt ttacgaaatc aaaaatctta
    70321 gacatgtctc ttgtcctgta tcgtcgggcc ttatactcat catacttcaa gttgcatgtg
    70381 ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg gattcactct
    70441 cttgtcgcct tcctgcaact cgaattattt agagtatgaa aaaatgcgtg ctctaaaagc
    70501 gcgctgaaac aagggcaggt ttccctgccc tgtgattttt tacttcttag cgcgctcttc
    70561 gagcatcgct actgcaggca gtactttacc ttccacgaat tcgaggaatg cgccgccgcc
    70621 agtggagatg taggagattt tgtcagcaat gccgaacagg tcgattgctg ccagagtgtc
    70681 gccgccgcca gcgatggaga acgcttcgct gtctgcgata gcgttagcca cgatttcagt
    70741 acctttgcgg aagttcggga attcgaacac gccaaccgga ccgttccaca gaatggtttt
    70801 cgcattcttc aggatttcag ccagttcctg agcggaagca tcaccgatat ccaggatctg
    70861 ctcgtcagct ttcacatcgt taacagattt cagggtagcc ggtgcagttt cagagaactc
    70921 ggttgctacg cgaacatcag acggaaccgg gatgttgcag gtggtcagca gacgtttagc
    70981 ttcgtcaacc aggtcagctt cgtacaggga tttacccaca tcgtggcctt gtgccgcgat
    71041 aaaggtgtta gcgataccac caccaacaat cagctggtca gcgattttag acaaggagtc
    71101 cagaacggtc agtttggtag atactttaga accaccaacg atagccacca tcgggcgagc
    71161 tggttctttc agtgctttac ccagcgcgtc cagttcagct gccagcagcg ggcctgcgca
    71221 cgcaacgtca gcgaatttac cgataccgtg agtagaagcc tgcgcgcggt gagcagtacc
    71281 gaatgcgtcc attacgaaca cgtcacacag tgcagcgtat tttttggaca gggtttcgtc
    71341 gtctttcttc tcgcctttgt tgaagcgaac gttttccaga acaaccagtt caccttcagc
    71401 aacgtcaacg ccgtcgaggt aatctttaac caggcgaaca gggttagaca gtttgtcttt
    71461 caggtagtta acaaccggca gcagagagaa ttcttcgttg tactcgcctt cggtaggacg
    71521 acccaggtgg gaagttacca tcacttttgc gccttgtttc agggccagtt cgatggtcgg
    71581 cagagaagca cggatacgcg cgtcgctggt tactttcccg tcttttactg gtacgttcag
    71641 atccgcacgg ataaatacac gtttcccagc aagatccaga tcggtcatct taattacaga
    71701 catggtgaat cctctcgttg attctaaaag ttttgcagac gctgcttgcg tcttacctga
    71761 aagcaacagt agccatagct aacgtcgtgt cgagcattcg gttagcaaag ccccattcgt
    71821 tatcgcacca gaccaacgtt ttgatcaggt gtgcgccact gacccgggtt tgggtgccat
    71881 cgacaatggc actgtgcgga tcgtggttaa aatctacaga gaccaacggc aattccgtat
    71941 agtcaactat accatgaaat gcaccttgtg ctgctttttg cagcaacagg ttgacttcat
    72001 tggcttttac aggtttcttc accgtcacgc ttaaatcgat tgccgtcaca tttatggttg
    72061 gcacacgtac cgcaatcgct tcaaagcgat cgttaaattg cggaaaaaat cgtgtgatac
    72121 cggcggccag tttagtatcg accggaatga tcgactggct ggctgcccgg gtgcgacgca
    72181 ggtcaggatg gtatgcatca ataacctgtt gatcgtgcat ggcggagtga attgtggtca
    72241 cagtgccgga ctcaataccg tacgcatcat ctaacaattt gatgacggga attatgcaat
    72301 tcgtggtaca ggaagcgtta gaaacgatgc ggtgttccgc acgaagttga tcctgattga
    72361 cgccgtaaac aacggtcgcg tcgagatcgt tactgccagg atgtgaaaag agcacttttt
    72421 tggccccggc ggcaatatgc gcttcgccat gctcgcggga gccatatacg ccggtgcagt
    72481 cgaggactac atcaacgcca agttcacgcc aggggagcga ttgcagtgaa cgttcatgca
    72541 atacgcggat ggcgtcatca ccaacaaaaa gttgatcgcg ttcctgtcgt acttcccatg
    72601 caaaacggcc atggctggtg tcatatttca acaaatgcgc catgcccgca gcatccgcca
    72661 gttcgttgat tgccaccacg gtaatttccg cccggcgtcc ggattcatac aaagcacgaa
    72721 ccacattacg cccgatgcga ccgaagccat ttatcgctac gcgtacggtc atagatctcc
    72781 tgcaaggttt tccctgagca aatttgccag acagagtaat ccagcaaatc gtccggggaa
    72841 accttacctg tcgcaaactg cgactgattg gttaattgtc gaacatttaa tcgactgaaa
    72901 cgcttcagct aggataagcg aaacgtggaa taaaaggaat gattgtccag tcaaagaaag
    72961 caattatctg acttacgtca cgttttcagc cccgcccctg tggaatttat tccactttgc
    73021 agaatcacga ataagcgtag caaaatcttt tcgttgtgcc gtcacaaacc ctgacttata
    73081 tcagaatatg actgatgcag cgtcgatatt atccgcaata cgtccgagtt tcggagtact
    73141 ccaatgatga cattgcccta cccatattct tcctccgcac gcttttacgc actgcgcctc
    73201 ctccccgggc aggaagtttt ttcccagcta cacgcttttg cacagcagca tcaacttcat
    73261 gccgcctgga ttgcaggctg taccggtagc ctgactgacg tcgccttgcg ctatgccggg
    73321 caagaaggca ccacgctcct gaacggtaca ttcgaagtga tctcactaaa cggcacgctg
    73381 gaacagagtg gtgagcatct tcatctctgc gtttccgatc cacacggcac gatgcttggc
    73441 ggtcatatga tgcccggctg taccgtgcgg accacgcttg aactggtgat cggtagcctt
    73501 gaggaactgg cgtttagccg ccaaccttgc gcgctttctg gttacgacga gttgcatatt
    73561 tcccccgtta aataactact ttcaaagagg ttttcttatg gcgcgtagcc acttttccag
    73621 tcaggcactg gtgttgatcg ttatatctat cgccatcaat atgatcggcg ggcaactggc
    73681 cagcatggtc aaattaccta tatttctcga ctccatcggc acacttatta gcgcggtact
    73741 gcttggtccg gtgatcggaa tgctgaccgg cttactgacc aatttactct ggggattgct
    73801 gaccaacccg atcgccgccg catttgcacc cgtcgcgatg gtcatcggcc tggtcgccgg
    73861 ttgcctggcg cgagcgggtt ggtttcgcac cctgccaaaa gtcgttgtca gcggcgtgat
    73921 tattacgctt gcggtaacgg tcgtcgctgt accgctgcgt actgcgctgt tcggcggtgt
    73981 caccggtagc ggagccgatt tgttcgttgc ctggatgcac tcgatggggc aaaacctggt
    74041 ggagtcggtg gcgattacgg tgataggcgc gaacctggtc gataaaattc ttacagcggt
    74101 gattgtctgg cttttactgc gccagctgcc gattcgcacc acgcgccatt ttccggcaat
    74161 ggctgccgtg cgctaaatgc atccatttac ctcattaaca ctgtgggcat tggcggcctg
    74221 caccacgctt atcctgcccg cgcagactat tttgccgatc tatagcgcgg cgaccttttt
    74281 ctgccttatt gcgctaaagg ccacgcggcg gcgggcaaaa tatgtcgtct ggctgatgtt
    74341 ctcgctggga gccgggctgt ggctggtaca cggtggctgg ctaccagaat ggctaagcgg
    74401 cacgccacgc tcgccagaac ggtggtcgca tgctattacg ctctggttgc ggattctggc
    74461 gattgtttca acctcacagt tgtggatgca gtacgtcccc gtgcaatgtt ttattcgcgc
    74521 tctgtttgct tctcgcctgc cgccaggcgt cgcttatctg tttgccgggc cgttattggt
    74581 cgttgagcag ttaaaacggc aattagcgat tattcatgaa gcgcagcgtg cccgcggcgt
    74641 gccgctggat gagggctggt atcagcgttt acgtgcgatg cccgcgttaa ttattccgct
    74701 aacacacaat gcgcttaacg atctggctgt ccgtggtgcg gcgctggata tgcggacatt
    74761 tcggatccac aaccgacgaa caacgttgtg ggcaccagcc gatagcaccc tacaacgcgt
    74821 agcgcgttac accatgatct tgctgatgct gacagaattc ggagcgtgga tatggttacg
    74881 ttagaacagt tccgttattg cccgacacac tcaacgcatc ctccgttctg ttatgacttt
    74941 cactatgtca aaccggggat ggtcgccatc tttggtgaca acggcagtgg caaaagtacg
    75001 ctggcacagc tgatggcggg ctggtatccg gactttctgc caggagagat aacaggcacg
    75061 gggacgctcc tcggtacgcc cattgggcat ctcccgctta atgaacaatc cgccaccatc
    75121 cagctcgtcc agcaatcgcc ctatttgcag ctctccggct gtacctttag cgtggaagaa
    75181 gaagttgcct ttgggccaga aaatctgtgt cttgctgaag cagagattat ggcgcggatt
    75241 gatgccgccc tgactctgac cgagtgccag ccgttgcgcc atcgtcaccc ggcaacactc
    75301 tccggtggag agacacagcg cgtggtcatc gcctgcgcca tcgcgatgca gccaaagctc
    75361 ttaattcttg atgaagcctt tagccgcctg actccgcagg ccagcgaaat gctgttgcag
    75421 cgcttacaac actgggcgtt tgaacgtggt tcgctgatta ttctttttga acgtcatcgc
    75481 acacctttcc tgaactactg ccagcaagcg tggcaattgc aaaacggagc gctccagcca
    75541 ctatgttaac gttaaataag atatcttatc gctggcctgg cgcggcaacg gattgtcttt
    75601 gcgacatttc tctgcaactc aaacaaggtg aatggttggc gctgaccggc gacaatggcg
    75661 cgggaaaatc gacgctgtta cgagtaatgg caggtcttct tacgcccaca gccggtacgg
    75721 ttatgctgca acaacaggcg atgaaaaacc tgaaaaaccg ccagcgcgcg gcaaaggttg
    75781 gcgtactgtt tcaggaagcg gaaaaccaac tttttcacag caccgtcgct gacgagatcg
    75841 catttggttt gaaactgcaa aaatgcccgg cggatgaaat cactcaacgt acccatgctg
    75901 ccctgcaatg ttgtcagtta gcagacactg ccaactcgca cccgttggat ctgcatagtg
    75961 cgcagcgacg gatggtcgcc gtcgcctgcc tggaagcact ctctccgcca ctattgctgc
    76021 tggacgagcc gagtcgggat tttgacgaaa attggttgag cgtgtttgaa agctggctgg
    76081 agaagtgcgg ccagcgaggc acgagcgtcg tagcaatcag tcatgacgcc gcatttaccc
    76141 ggcgtcattt ctctcgtgtg gtgcgactgg aagatggtct aatcaggaac gttaatccac
    76201 cagacgatat tcacccgtag cggtcatcat taaggtcaaa tttgcaggca gactctcttc
    76261 cagcccccgg cgaacattcg gcccgtcggt acggtcataa aaagcttctg cgtctgccag
    76321 cgatacccct cccgccagct tgcgccctac cagtcgttcc cgcaacgcag tagctggcgc
    76381 gttgataaag atcgagaaat cacaaaattg cgcaagctgg caccacttct cgtcgtccag
    76441 caacaaccag tttccttcga cgatgacgag tggtgcggta acgtgcaacg catcttcaac
    76501 aggatcatgc ttttgtcgat catactgcgg ccacgtacaa tccccttcca ctacctggca
    76561 cagattttcc gccagtttcg caacgtcaaa tgtctctggt gcgcctttga aggggcgcaa
    76621 ttgatgcgca tccagccagc tattgtaatg gtgaaaacca tccatcggga gcgtctggat
    76681 agcgggtaat tcagggtctt gttgcgcgag atattcccag aaggtagtta aggtagattt
    76741 acccgtcccc ggcggagcgc aaagaaaaac caccgtacgt cgctgcggac ttaccgtctg
    76801 taaggccgcc agcatacgca gcaacggttt atgcacgcgt tctatctctt catcatggta
    76861 ctgcgcttgc acgttcagcc cattcaccgt taattcaatt ttcacggcgt taattccttt
    76921 aaggtggtcg ttactgccag cgtcagcgcc gttttgacca ttccgctaaa acgcgcctct
    76981 gaccagacgg cgaaatcggc gaacttcatc gcttttaacg cgtcatagag cgtcgcattg
    77041 caggttatgc tattgacgtt gctgataaaa tcccaggtca tgtcatcggc aaatccgggc
    77101 gtttcatcct gttcgttcag gtaatgccag tactgggaaa agtgcgtgat atcggcatac
    77161 agccatttat ccattttccc cagcgcataa atcaaacgca gcgcaacatc aatatcgtca
    77221 agcgggccgc tttgggcgag taacggcttc actgcatatt cgacgatctc ttcatcgtta
    77281 tcgacaaaca gcgacggcag aaactggcgt atcccctgga gcaaaataga atgcgcagtt
    77341 ttcataaatg aaaataaatt gtcctgtgca tccagttgct caagcacatc atcttctgtc
    77401 agcgtcgcca ttggtcttcc gatagatcac catcaatgca gggagtctat tatattacac
    77461 gtttggtccg cgccgccgat cagtaatgtc tgcgtccgca ccccattcgc cgtctgttgg
    77521 ataccgttca ccaactcgcc gcccgtcacg cccgtagcgc taaagaggat atcgttcccc
    77581 ctcaccagtt catcgagcga gtacacacgg ttaacgtcaa cgcccattgc cttacaacgc
    77641 ttgcgctcct gctctgcaat ctgccgattt tccgtgtaat cccctttagc ctgacaaaag
    77701 tcaatcagct cggcctgcat atccccacct aatgctttaa cggcacaggc agaaatcacg
    77761 ccttctggcg caccgccgat ggtgtacatc acgtcgtaag gattatcctg ccagcaggtc
    77821 agcacgctgg cggcgacatc gccatccggc agagcgaaaa ccttcacgcc cagctgtgtt
    77881 gcttcttcaa tcgcggcact caggcgcggt ttatccagcg taaccatgcg cagcttgtcc
    77941 agcggtttac ctaacgcccg cgccacgtta cgcaagttgt ccgccagagg caaagacaga
    78001 tcaatcgccc cggcagccag tcgattaaca accagctttt tcatatacat atcgggcgca
    78061 tgtaacaggc tatctcgagg ggcgaaagcc atgaccgcca gcgcattgct ctgccccatc
    78121 gctaccatcc gcgtgccttc aatcgggtca accgcgatat cgacttctgg cccatcgcct
    78181 ttgcccacct cttcgccaat ccacagcatc ggcgcatggt caatttctcc ttcgccgata
    78241 accactcgtc cacgaaaagc gacgtcgttt aatgcctggc gcatcgcggt gaccgccagg
    78301 ccatcaattt tatttttgtc gccacatccg gtttgcggcc aggcagccaa cgctgcctgt
    78361 tccgtaacgc gaaataatgg ccacgccagg gacatcattg cgcggcctcc ccgatatcaa
    78421 cgccaaattg tgccagcaga tatttctcgg cctgttcatt ccagatgccg tgcgtttcct
    78481 caaccagccg cgccaactct ttaaacagcg gatcggtttt gcctttttcg gcaaaatcgg
    78541 caatggcagt gagcggcatg gtcacgccgt tataaatgag ttttttaccg ccagggatat
    78601 ccggcagatt gagcacggta tctggcaccg catccagccc accgatatgc gtgaccataa
    78661 aggacggctg caactgcccc gtggcgctta acgcaatcgc ctctttcatg tcgtccgttg
    78721 aaccgccgga tgtaccgact acgtgcgtgc tgttgtaatg gacgttgtag aaattaaacg
    78781 gcactttgaa gtttttatcc gtcggcccgg caaagaagtt cagacagcca tcttccgcca
    78841 gtaattcgtc agccatctca acgacagcag gcaccgccgc ataaacaaaa atgtcatcga
    78901 acccgacatc tcctgtcagc gcccgcagcg tctggaccgg atcgctcatc cctttggtat
    78961 tcacatacac cagctcaatg cctttactgg ccgccagatc caccggcagc agcttctgca
    79021 cctgcgccag gcgtttgtcg tcgatatcga ccaccaccac ccgtgacggt tgtatgccac
    79081 cgttaatggc gtaatcgata gcgccaatgc ccatcggacc cgcacacgcc agcagtgcaa
    79141 tattgccgcc aggtttgacg cccatgcgat gctcataaac atattgcgtg gtgtgataat
    79201 tggcatgata agcaccaata atgcagcaca taggctctgc cagcgacgcc gcagcaaaat
    79261 aagagccgtg atacggtaat acgcagccca aattaatggc gatttcggga ataatcatat
    79321 aggtggcgtt accgccaaaa tattcgtagc tgtagcctgc tgaatatccg cttggtaacc
    79381 ccatcgccgg ttgcaataca aaacgctggc cttttttata tttgccagtg agatttttac
    79441 ccacttcgac aataaccccg gcacattcat gcccggtaat gaccggatga ttttctaaat
    79501 cgtcgggtac gcgtttatgt tcactaccga gtaacgccgc tttccaggtc gataaacaga
    79561 cgctgtcaga aattacactc actaacagtt cattatcggt aatttctggc agttcaaatt
    79621 cgcgcagacg gacatcccgc ttgccataaa tagcagcaac tttggttttc atttttacct
    79681 ctgtaatcag tgtttatgtt cggcggttaa ttgattaaat aaagtgtcga gttttggatc
    79741 gccaatatag ttattaatca atatcagcgg tttatccgtc acacgtttca cgcgcccttc
    79801 cagactggcg tgagtgacga cgatatcggc atccgcaggc acgttttcta tcgcgtaatg
    79861 ttttacttct attgccagcc ccgctttttc caggcgttta cggaaggtgg tcgcgcccat
    79921 cgcactggag cccatccccg catcgcaaac aaaggcgata cgcttaaccc tgcttaacga
    79981 gaatgcaccc tcttgtttca tcgccttaac cgcattggct gactgagcaa actcatcttc
    80041 gctctccgtt tccaccgttt tttccatctt cagtatcagc gaagtaatag caaaggacac
    80101 cagggtacct accgtaacac cggcaattgt cgccaggaac gagcctttcg gcgtcagtgc
    80161 caggtaagca aagatagacc ccggacttgg gccagccacc agaccaccgt ccagtaagtt
    80221 aaacatccag gtaccagaca taccgcccgc aatcatggca ataatagtca gcggcttcat
    80281 cagcacatat gggaaataca gttcgtggat cccaccgagg aagtgaataa tcatcgcccc
    80341 gggcgcagaa cgtttactca tccctttacc aaacaaggta aacgccagca gcagccccag
    80401 gcccggacct gggttagagg ccaccataaa gaagatggat ttaccgttaa ccgaagcctg
    80461 ttgcattccc agcggataat agacgccctg atcgatcgca ttattaagga aaagcacttt
    80521 cgccggttca ttgatgactg acagcagagg cagataaccc gcatgtacca gcgcctcgat
    80581 acactctttg acgaaagtat tggcaattaa caccgccggg ccgatcactt caaaacccag
    80641 cagacaaagg agcatccccg cgatacctaa tgagaagtta ttgataacca tctcaaaacc
    80701 ggcaggtatg cgtttttcca gtgccttatc gacatatttt atgaccaacc caccgagcgg
    80761 ccccatgatc attgagccaa ggaacatcgg gatctctgcg ccaacgatca caccaatagt
    80821 acctattccg cccatgaccg ccccgcgttt accgccgacc agatgaccac ctgtagaacc
    80881 aatcatcacg ggcaataaat aggtaatcat cgggccgaca attttggcga aatgttcgtt
    80941 aggcagccaa ccggtgggaa taaataacgc agtaataaaa ccccaggcaa taaaagcacc
    81001 aatattgggg atgaccattg cagtcaaaaa gcccccaaaa gcctggacct ttgcacgagc
    81061 agacttgttt tccataatat tatcctgtag aggagagagt aattatccgc ggctgataat
    81121 gtccgctaat tgtttttctg atgatgctgt taagagttgt tcgagtattt cttcttcgca
    81181 cagtaattca cttaacgcct gaatagcgcc aatgtgcgaa tcggcatcgg cagcagagag
    81241 tccgatgagt aatttaattg gttcgtcatt ccccggaaaa taaacacctt gttcaagtaa
    81301 tgtcaaagac atcccggttt taagcgcccc acattccggt cgcgcatgag gcattgccac
    81361 gcctggtgcg agaatataat aagggccatt gttaatggtg gaatctttaa ttgcctgaat
    81421 gtaattctcg ctgatatagt ttttatccag caatgatacc atcgagaaat cgatagcttc
    81481 ctgccaatct tttgctgaat gtataactga gattgatgat tctggaaaat aatcactaag
    81541 ccgcataaat tatccttatt ttatttttag ggtaccgtcc ggcggtaaaa ataaaatact
    81601 cgtgcggttt aatgaaagca attgagcagg gaaaccgatg ttttgaaaac agtgaattat
    81661 taatttctta tataacattg agttatagat ataacaacaa atgtcaatac gcatatcgtg
    81721 atgcatatta cataattgat atttattgca tataaatatt tgtgatctac aacacgcttt
    81781 atctattgct tgtccgcaaa cggacatatc aaggtaataa aaaaggtcgc cgaagcgacc
    81841 tttttacccg aaatgctaat tacagcagtt cttttgcttt cgcaacaacg ttatcaacag
    81901 tgaagccgaa ctcttcaaac agcagctctg ccggagcaga ttcaccgaag gtggtcatac
    81961 cgacgatagc accgttcagg ccaacatact tgtaccagta gtcagcaata cccgcttcta
    82021 cagcaacgcg tgcagtaacc gctttcggca gtacggattc acggtaagca gcatcctgct
    82081 tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc tttcacgcct tcggcagtca
    82141 gtttttcgta ggcagcaaca gccagttcaa cttctgaacc ggtagcgatg aaaatcagtt
    82201 ccggctgacc ggcgcagtct ttcagcacat aaccaccgcg cgcgatgttt gccagttgct
    82261 cttcagttcg ttcctgctgc gccaggttct gacgggagag gatcagtgcg gtcgggccgt
    82321 cctgacgctc aacaccgtat ttccacgcga ccgcggattc aacctggtca cacggacgcc
    82381 atgtagacat gttcggggtt acgcgcagag aagcgacctg ctctaccggc tggtgagtcg
    82441 ggccatcttc gcccagaccg atggagtcgt gggtgtaaac catcacctga cgctgtttca
    82501 tcagcgcagc catacgtacg gcgttacgtg cgtattccac gaacatcagg aaggtggagg
    82561 tgtacggcag gaaaccaccg tgcagggaga taccgttagc aatcgcggtc ataccgaact
    82621 cgcgaacacc gtagtggatg tagttacccg cagcatcttc gttgattgct ttagaaccag
    82681 accacagggt caggttagac ggtgccaggt cagcagaacc gccgaggaat tccggcaaca
    82741 gcggaccgaa agcttcgata gcattctgag acgctttacg gctggcgatt ttcgccgggt
    82801 tagcctgcag tttagcaatg aactcttttg ctttcgcgtc gaagtcagac ggcatttcgc
    82861 ctttcatacg gcgggtaaat tcagcggctt cctgcggata agctttcgcg taagcagcga
    82921 atttctcgtt ccatgcggat tctttcgcct ggcctgcttc tttcgcatcc cactgagcat
    82981 agatttcaga cgggatttcg aacggtgcat atttccagcc cagctgttcg cgggtcaggg
    83041 caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg
    83101 aaccgaaacc gatgatggtt ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg
    83161 cttcttctac tgcgcgtttg atggatgccg cgtcatgacc gtcgatgtcg cgaataacgt
    83221 gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt gaaccagcct tcaacgtgac
    83281 catcaataga gataccgttg tcatcgtaga acgcaatcag tttacccagc ttcagcgtac
    83341 ccgccagaga gcaaacttcg tgggagatgc cttccatcat gcagccgtcg cccatgaagg
    83401 cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt
    83461 tttctgcaat cgccatacct actgcgttgg caataccctg ccccagcgga ccggtggtgg
    83521 tttccacacc agcggtgtaa cccacttccg ggtggcccgg agttttagag tgcagctgac
    83581 ggaagttttt cagttcttcc atcggcagat cgtaaccggt gaggtgcagc aggctgtaga
    83641 tcagcatgga gccgtggccg ttggacagca cgaagcggtc acggtcagcc caggacggat
    83701 tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc ggcaatgtca gccataccca
    83761 taggggcacc cgggtgaccg gatttggctt tctgtactgc gtccatgctc agcgcacgaa
    83821 tagcattggc aagctcttta cgtgaggaca ttttgactcc agatcggatg atgaagggca
    83881 cgcccttaac gacttgacga cagcgcgttt tgggctacgc cggaaaattt gccaacaatt
    83941 taccgcaagc cgcgcgtcat gtacatggaa catccttttg ccgcttcaga aatctctgga
    84001 tcatgctcgc atgttgcgca atctactcgc ccgtccgctg cgcttttcct tatactgaga
    84061 ctgagcgtcg attcacctgc aaacggcgca tttttagaat aatcctgacc ttgtgcggaa
    84121 gagaaaacat gaaaattcgc gccttattgg tagcaatgag cgtggcaacg gtactgactg
    84181 gttgccagaa tatggactcc aacggactgc tctcatcagg agcggaagct tttcaggctt
    84241 acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca
    84301 gcaaggcgac gattgcgcca gccaatagcg aatacgctaa acgtctgaca actattgcca
    84361 atgcgctagg caacaatatc aacggtcagc cggtgaatta caaagtgtat atggcgaagg
    84421 atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt ctatagcggg ctgatggata
    84481 tgatgacgga taacgaagtc gaagcggtga tcggtcacga aatggggcac gtggcgttag
    84541 gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa tgccgtgcga gtagctgcgg
    84601 cctctgcggg cgggattgtc ggaagtttat ctcaatcaca acttggtgat ctgggcgaga
    84661 aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga agccgatgat tattcttacg
    84721 atcttctgcg ccaacgcggc atcagcccgg caggtcttgc caccagcttt gaaaaactgg
    84781 caaaactgga agaaggtcgc caaagctcaa tgtttgacga ccatcctgca tccgccgaac
    84841 gcgcccagca tattcgcgat cgtatgagcg cggatggggt taagtaagtc agacataccg
    84901 atctacaaca taattgtagg tcggtaacgc ctgaatcccc gatcagagta gtgatttcac
    84961 tttatttagc aaaacggcta catcatccgg tcgggtatat cccgtatcag ggtctataat
    85021 cgagctgtaa acatgtggca taatgcgcgg cacccccgcc tccagacaag ttttcagaat
    85081 cacactgaag ttatccaaat caatcccgcc agtcggctca atcagcgtca taccattgcg
    85141 cgccgccgtg gtcgccagcg cgtatagctc cggaagcgac ctctcgccgc ccatcggaaa
    85201 gaacttcgcc gcgtgtgcgc ccatatctag catcattcgc accgccgtat cgcaggagac
    85261 gcgggcaggc gtaccctggc tgctgctaac gccggtggaa atcagcactt cgcccggcgt
    85321 ccccgtcggg ctaaccagcg cgttgacatg ggtctgctca ccgcccgtag ccgcaagcgc
    85381 gcctgccgcg aaaccacaac cggtaaaggt ctggttaacg tgtgctggat gaatctgcgc
    85441 cgcaatcatc gcagctttgt aatactgcgc cgggtcaccc gcccccagac ctactgaaat
    85501 ggacggtatt tccgccatcc aacgtttaac ttcctggatc ccctcatcca ccgaggagaa
    85561 ctgcgcggaa agcacgccaa tcactgcgtg gccttcggcg gcgtcaaaga tttcacgggc
    85621 attggcgata tctttcgcca gcacattaat tgccacgcga ttacgataga aattaatctt
    85681 ttgcatgatc tgcaatctcc ttcagtcgcg ccacgataag tgccatttcc ccttccgcca
    85741 ccgtacgcgg atcaaggcta aaaacgccct ggtggaggtt ataccgccgt gcataaatcg
    85801 cgatatcgcc accacgcagt tgcgcttcga cgacgcgagc gtcaactccc aattcctgcg
    85861 catttactcg aatacgaata cgccagattg cacgcccggc ttcatcctgc tcaatatcag
    85921 ccgttaaccc gtgaatcgca gaaatcgctt caaccactgg ctggagctgt tccgcggtga
    85981 tgactgcctg cccctggtga taattctcca gcgcgtaaac cagcccaacc atgttctcct
    86041 tgccgatttt catcgccctg gcaatgccgt gatgctgcgc tttgcaggct gcaatccaga
    86101 tttttttacc agtgataaac ccggaggtcg gcgcgttaaa ggcttttgcg ccgctgtaga
    86161 ccaccatatc cgctccgctc accacccagg cacgtaaatc ctcttcggcg gcggcatcga
    86221 caatcagcgg caggttgtac atctgcgcga catgtgcgaa gtcctcgatg ttcaacattc
    86281 ccttctgcac gcagtgatgc gacttcacat acagcagcgc ggcggtcgcg tcggtaatgg
    86341 cactctccag ctgccagaga gccgccaggt tacttgaacc cacctccaca acgcgcccgc
    86401 cacctaagcg gatcgcgctg aggatcgatg caccgtaatc gacgttatgg ccgcgcagca
    86461 tcaccacttc attggccatg ccggagctat ccggcatgag cgccacccgg gccttgtcgc
    86521 cgcgtgtaat ggcggcggcg acagcgatgg cgatccctgc tgaggcacag gaggtaacgt
    86581 agctatcctc cgccccggta aagcgagaga ccagctcgcc ggttttgtcc accagctggt
    86641 caatctccac gaaagaggat gccgcgcgcg cggtggcctg catcacctcc ggcgcgacgc
    86701 tggagacgcc gagaatcgtc atcttgccgc aggcgttaat cacctgtttc agtcccagtt
    86761 gttgatagat attctgcgtc atggcttaca gcactcccag cagcgagcag accacgctca
    86821 acgccacaat cgacagcaga atcgtggtgt aacgcggacc ctttttcacc aaatagaagt
    86881 agatggcgaa cactgccgcc agcggcagca ggccgggcgc gatggagtcc agaatctgct
    86941 gtaccaccac ttcagagcct ttcagtgcgc taatttttaa aggtgaggtt atcttgacaa
    87001 agctcgcgga tagcgcgccc atcatgataa gccccagtac gttagcgccg tagataagct
    87061 ccttgatgcg ccctccctgc agcagactga taatcgaatc gcgccccagc gtatagccct
    87121 tgtgcaccat gccgtagctg attgccagcg taatcgccgg gtagagaatc agcggcaaaa
    87181 taccgcccat cgcgctgcca ttggcggcga acgggataaa aatagcaatc aacagcggca
    87241 tcaccgccgc ccagatgatg gagtcgccca tcccggccag cggccccatc aggccggttt
    87301 taatcccggt gatagaggca tcgttaatcg cctcgccgcg ggtcttttgc tcttccatgg
    87361 cgatggagat cccctggatc accgcgccaa acgtctgctc tgagttgaag aagttcagat
    87421 ggcgttttaa cgcctccacc tgctcctcct tttgcggata gagctttttg ataatcggcg
    87481 tcatcgaggc gcagaaaatc aggctctgta aacgctcata ggagctggaa acttccgcgc
    87541 ccagccagta gataaaccac gctttggtga tatccgcttt ggtcagcgcc ccggactccc
    87601 gcgcgcgctc aaccagttca tgctgcatta cgtcgtcgct cattatgccg ctccttcatt
    87661 tttcgccagg cctttaatca ggaaggccac gcaggtgccg aaaatcgcca tcgccatgat
    87721 gtccaccttg aggtacagca ccgcgaagaa tccgccgata aaccacggca gcaggctctt
    87781 tttaccaatc accataatgg tgatggcgaa gcccagcgcc gggagaatcc cgcccatgat
    87841 ttcaaacgag tgggtcagcc agtgcggcat cagcttgagg aaactttcca ctacgttctg
    87901 gccgaagtag ttggcggcga acaccactgg gaaacgtagt accagcccca gcagcgccgg
    87961 gtagagaaag gcgcagcgca taatggctga catattggcc gtttccgcgt gtttatcggc
    88021 catatgcacc caggccgcat ttagcgtgcg acgcaactgg tcgaggaata cgccgataac
    88081 gccaaatgga atcgccagtg caatcgccag gttcgggtcc atattggcct taacggcgat
    88141 aggaatggcg atacaggccg ccagcgccgg gtctgacggc acattgccgc ctggcgttga
    88201 ggtcacaccc agatagacca actgcatccc cgcgccgata atcatcgccg caggcatatt
    88261 gccgagcagc aggccgacaa agaccgccac caccactggc tgtaacagca tggcggagaa
    88321 ggtatagcca aggcgcaacc gggcaaacca gtaatacagc cccatcaggc ttgcaaaaag
    88381 taaggtatcc ataatttgtt atcccatgtt cagagggtca gaattttttc aggatatcgt
    88441 ccagcgactg cggtttatct tccgggatgg tctggaaaac agccttaatg ccgcgacttt
    88501 tcaaatcgtt aagaatgcca acgtcttttt catccagcgt tatgttctgg aacaccgctt
    88561 tacggtttgg cccgccgcct aaaccgccaa cctgaatatc agccacgtca aagccctgct
    88621 gcaccgcttc ctgaatcgcc gccagcgacg ggaacagcac cagcacgttg ccgtcgccca
    88681 gctggttctc tttccacgcg gccgcgaagc tctggttgct aaagcagtcc actttgatat
    88741 tcggcggcgc ggccatcagg tagatatttt tcataaacgg atcggcgtcc agctcgtcgc
    88801 tgaccaccgc aatacggttg gcctgagact gccctaccca cttcgtcacc acctgtccgt
    88861 ggataagacg gctgtcaatg cgacataaca cgatgtttgc catacgattt tccttactgt
    88921 tgtaattgac gaatatggga gacgacgtcc aggcaactgt tgcgcccagc ttccaccaga
    88981 tcggccacgc aggcggtcag tgggccatgt tcgcgcttgt ccagcgcctc cagcagcagc
    89041 gaggcattaa gcccggagat caccgccacc ggataatcgg cgctcagccg cgccgccacg
    89101 ttagaggtgg tgccgccgat aaaatcggtc acgataagcg aaccttccgg catggttttc
    89161 accacggctt cgacgcgctg gtaaaactcg cccagcgtat cgacgggcat cagcgcgatt
    89221 tccgtcacgc cttcgatttc gcccatcacc atgcgcagac tgttgcagag ctgcagcccc
    89281 cagccgccgt gagttagcag cagaatttca ggtaacggat gaataggtac agttgtggtc
    89341 aaaatagcct cctttgctcc agtgtttaca tttcatcagg agcaaaggac gtgccagaat
    89401 ttgtgtcacg cacagaggaa atgagattgg atggggcgtt aagccccgta aaccagggga
    89461 tcagctgtag agcagttcgt agatataaaa atattctgcg tccgataaac ggatagcgta
    89521 agcctcttca atcggcaaaa aggaggattt gatgacgcta aaggcacgag cgtccagatc
    89581 aagccggttt tctagcgcca tctgtaacgg tttgcggtta atcacgatac gctcgaccat
    89641 gcagcagcag tggatcagaa aacgtagtgt cacctggcgg ctcggtttca gggagagcgt
    89701 gttggttaag tagttcagaa catcctcgat ctctttcaga attcgctgcg ggttgagcac
    89761 cgaaatatgg ttgatgatgc tctccatggt cagcgcgctg ataaagcgca tggcgctgcg
    89821 ctccatctcc agtcggcgct cgctgcttga gagatccggc gtcagcaggc tgagcaccag
    89881 ctccggccct tgctcggaaa acagttcctc cagcgagata aacggtatat ccggcagtcc
    89941 cggctggaag gtgccgacga tccctgccag ccgttcactg gcattaagcg cctgctgaat
    90001 acgctccagg ctgcgcacct cgttatagtc gagaatcacc atacgcgtgt cctgcgacat
    90061 cagctcgcca aagctctcct ccagcacctt tttgatcttt tccgccgtcc ccattccggt
    90121 tatacatgag ataaccagca ctttgccacc gctctcttgc tgcggcgtgc agagccgaca
    90181 agggatattt ttactctgca taagcacagc caattgcgat agatcgccag tttcgtagct
    90241 taaatccagc ccaacctcca gcaggctggt cagagtgata tttggcaaca acagaacgtc
    90301 aatctggaac agcttgctga tggtgctgcc aaaatggacc agcgatccga tatccaccat
    90361 cagtatcagc cgctgccagc accggtcctg aatcatccgg gtcagggttt ccagggtgtc
    90421 atgcaccgac tgctcaaatg gcatatcaat tgcgtagaac agatcgcgct ccagcacccg
    90481 gttcacatat tgcgcctggc tcgttgcggt ggtcgcaccg tgagcgatta gaatcacgcc
    90541 gcaatccggg ctggtgtcga tgcgctggcg gtagtggcga cactctttca ggaacaggca
    90601 cagccagacc acctccgtcg ccgggcactg aatatgcaac aactcattga ttttgcggca
    90661 cagaagcgtg gcattgtcat actcatcttt acagcgatcc agaatcaggc tggatgaata
    90721 gagttgaggt ataagccctc gccgcacata gccaatcagc gcaagaaagt gtttacgtaa
    90781 cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc tgctccacgt aaccgatcag
    90841 cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt ggatgcgccg cactatggcg
    90901 gctataaaga ccatattcaa aaatcgaact gagtttgttt ttcaggatag ccagcgtttc
    90961 tgcgggcggc acgttactgt tgcgcagatt gacatattca cgagtcagga aactgtaaaa
    91021 caggtcgctc tcttctattt ccgcgctggt cgccaacgaa ttttttaacg cgggcaacgt
    91081 acgcgcatcc acgttcagac gatctttacc gccaaacaac gcatcgacca gcagacgctg
    91141 ctctggcgtg gtattaaacg acatctccgc cagtcgttta tctaactcta gcgtatcatt
    91201 acgctcggtc attcccgacg cccacgcctg cgcgcacaag aactgaatat cacttttaag
    91261 ctggccgata ttgccttcca ggggtttact taacaaccaa tgcaagagcg ttttatcaag
    91321 gcttatggta cgttcaattt tacgactttc acgctgaaag aagcttacaa tcaattcgag
    91381 ctgctcctcg accgaacgct ggcgaatacc cggtaagtca atacatacct ggatacgccg
    91441 ctggaaggta cgcaatagtg ccgagttgac cgactctgta gtggcgcaaa taaggcgcac
    91501 cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg tattcacctt tatcgagaat
    91561 agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc acttcatcca gaagtaaata
    91621 acctccgtca gcttgctcca ccaatcccgt tttattctca cttgccccgg taaatgcccc
    91681 cttatggtga ccaaacagat gtgaggaaag caactccgga ttatgagcat attcggcaca
    91741 gttaaagtaa accaacggag gtggcgaacc tatggtgtgc ttgcaagcga agcaatgcat
    91801 taactcagca aagaatgttt tgccaacacc agatgcaccg gttaacagca catgtagtcc
    91861 gtggggataa agcaccgctg cgcggccttt ttctaccgca tcacggagac tacggtcgta
    91921 tccgataagt ccggaaaacg gatcatcatc tgtatatttt tctttctttg gcaataaact
    91981 agccactgaa tgaactatgc attcgcaatc atccagtttt cgattaatga gcaattctag
    92041 tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt tttaccacca ggccttcatt
    92101 ccataattga ttaagatctt tactaaccga gttgcgtgcc agcccaagat taaaaccaat
    92161 cgcctcagca gtaaatgctt tctccttcgc taaatcagac agttccagcc catacgtcag
    92221 ccgctccagc tccttgtgaa taacatcaat ccgtctcatt tctttaccgc ttaaaaaaca
    92281 atagttgttc agaacataca ctatttgtaa acgtaagaat gagaactatc gggaagaatt
    92341 aatgttcctt gtggcaatct ggtttctgaa tattcgtgtt ttaaatatta cttatggtct
    92401 gaaacaacca ggcaggtgaa cagagctaag aaaaagtagc gtagaaatat aaaaacatat
    92461 atcgcactga tgaatgtttc ccggcatgac tatgcacacc gggaaatatt tagtaaatta
    92521 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
    92581 agcagtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
    92641 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca gaccgccaat
    92701 taccggcgta ccggttcccg gcgcaaatgc aggatccagg cagtcgatat caaaggtcag
    92761 gtaaaccggc atatcaccca caatttgttt cacctgggcg ataacgtcat ccacgctgcg
    92821 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa attcggtacg
    92881 aataccaatc tgcacggaat gattcggatc gatcagacct tctttcggcg cggtatagaa
    92941 catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg
    93001 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
    93061 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
    93121 ctgcagcttc tcgctcatct cacgggcatc gccgaaagca tataccagat caccgcagtc
    93181 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
    93241 cagattcgtc gaaacctgac ggatagctgc cggaccgtgg cgtccccccg cacgaccaga
    93301 agtggccata tcgaacggca caccagtaat cacccagtca gcatcgctgt catacggctg
    93361 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
    93421 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
    93481 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
    93541 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
    93601 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgcctg
    93661 gccttgtaat tcaaaggcgg caaagcctat tacaccggta ttacgcacat cgccttgcgc
    93721 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cggtatgttt
    93781 atctttgcgg aatcctttaa tcgctaaaga atctccggca ttcacaatat agccgttgtt
    93841 attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcaacagtag caacgatctc
    93901 gtagttggtg ttacgactgt aattacggac atataattga taattcattc cgactctggc
    93961 atggacaata tagcgtggtt cattattgga tgaatcataa acacgcgtaa taggtagaga
    94021 attaaaatta ccatcgcgaa tagcatattc cagttcgtta atacgaatgc tataaacgcg
    94081 gtcatagccg gaaggaacat tagtcgaata actaataacg atcatctctg ccggatcgcg
    94141 ccaggctcgc tcagcagata cgccctgaac cgatgagtgc acatccccac cccaggcagt
    94201 gccgacattg cccgatgcac tgctattata ctgcgcatta ctggaacaac cggccagcag
    94261 cacgattaat gccaccaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc
    94321 atttaattaa tgcagcgatt gtagggagat tatttaataa gaaaagcagc gagattattt
    94381 ttaagaatat ataaaaagaa agcgggccga tttacgtaaa taaatcgacc cgccagcgga
    94441 ataatcttaa ttattcatct tcaagatagg tataaccgta caaacctgcc tcgaactctt
    94501 caaggaactg ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact
    94561 gggttaacag cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc
    94621 cttcgtcaga cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt
    94681 cggtatcacc gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa
    94741 agaaaccgag catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa
    94801 taccatcacc atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca
    94861 gcactgcgcg acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact
    94921 ggtcgatccc ccatgcatcc ggcattgact ggaacagcga gaaattgacg tacattttgt
    94981 ccgccatacg ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca
    95041 gctgcttttg cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt
    95101 gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt
    95161 cgtgtaacca ttcacgcaga gaacggcgag ttcccggttc gtgcatttcc tgccaggttt
    95221 cccacatgtt ttgcagcgcg cgcggcgcat cttctgcagg cgcggtcggc accgtgtatt
    95281 cgttacgttc cacgccgatg atattagata ccagcacggt gtgatgcgca gtcaccgcac
    95341 gacccgattc ggtgattacc gtcggatgcg gcagaccgtt ttcttcacac gcatcaccaa
    95401 tcgcccagat gatgttgttg gcatattcat tgaggccgta gttcaccgaa cagtcggact
    95461 gcgaacgggt gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat
    95521 tgacgcccag cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cctgtcgcga
    95581 tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg cagcagttgc aggctgtcga
    95641 gacgcccggc ttcacgcagg gtttcaacaa gttgcagtac ctgagtcgca gccaggccga
    95701 acttcgattt ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg
    95761 cacgcacgcc cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga
    95821 tttctgacat cttctcaatg accaggtaga ccttgtggcc catcttctcg ccaattaatg
    95881 ccaggcggat atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc
    95941 cagcatgtgc cagcaccgcc atcaactctg ctttcgagcc tgcttccagt cccagcggtt
    96001 cgcccgaatg aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa
    96061 caaggaagta atcgccgtta tagccgtagg attcccgcgc acgtttgaac gcggcgttaa
    96121 tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac
    96181 cctgtgcttc acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg
    96241 ggtccgggca cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc
    96301 aggcaatatt gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct
    96361 gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt
    96421 cagacatagc gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc
    96481 ctggaactgc gataacaacc cataagcaca tggattttcc agcagtgtat gctgacgctc
    96541 cgactgcgca accggtttct ttttcataac attattaagc acataaccga acgtaagtgt
    96601 gacagttcgg cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc
    96661 tcagtaactg taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca
    96721 ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag
    96781 gttaagcagc agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg
    96841 aacccacaag ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt
    96901 cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc
    96961 aaaagataaa tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac
    97021 agaatgagac acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc
    97081 atccagatgt taatccatcc ataccgatta acactcagac tgccagtgtt tttaacttgc
    97141 agagtcgtgg taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat
    97201 tctctttagg tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa
    97261 gggcatcctg acaaaattgc tgaccaaatt tctgatgccg ttttagacgc gatcctcgaa
    97321 caggatccga aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt
    97381 ggcggcgaaa tcaccactag cgcctgggta gacatcgaag agatcacccg taacaccgtt
    97441 cgcgaaattg gctatgtaca ttccgacatg ggctttgacg ctaactcctg tgcggttctg
    97501 agcgctatcg gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg
    97561 gaacagggcg cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg
    97621 ctgatgccag cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt
    97681 aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat
    97741 gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag
    97801 atcgaccaga aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc
    97861 gctgaatggc tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc
    97921 ggtggcccga tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc
    97981 ggcatggcac gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt
    98041 tccgctgcgt acgcagcacg ttatgttgca aaaaacatcg ttgctgctgg cctggccgat
    98101 cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc catcatggta
    98161 gaaactttcg gtaccgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc
    98221 ttcgacctgc gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa
    98281 gaaaccgcag catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa
    98341 gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg
    98401 ccagcctcgc gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca
    98461 tccggcaaaa agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat
    98521 tatcatcact actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc
    98581 atattattat tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga
    98641 taacagcgtt taatctatga tgatataact caattatttt catgcactta aaccataact
    98701 aagataaatg ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc
    98761 gtactcacct atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac
    98821 gctaaaaaac aggggcggtc aaacaaggca atgacttttt tcgtctgctt ccttgccgct
    98881 ctggcgggat tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt
    98941 gcagatgaat tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc
    99001 ggtgcggcag tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag
    99061 agcctgatga tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca
    99121 aacgttgaag tactgattct ttcccgcgtt ctgctggggc tggcggtggg tgtggcctct
    99181 tataccgcac cactgtacct gtcagaaatt gcgccggaaa aaattcgcgg cagtatgatc
    99241 tcgatgtatc agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc
    99301 ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg
    99361 ctgctgattg gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caagcgccgt
    99421 tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc
    99481 gaactggatg aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa
    99541 gagaacagca acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa
    99601 ttcaccggga tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat
    99661 accaacacta ccgagcaaat gtgggggacc gtgattgtcg gcctgaccaa cgtacttgcc
    99721 acctttatcg caatcggcct tgttgaccgc tggggacgta aaccaacgct aacgctgggc
    99781 ttcctggtga tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtattcac
    99841 tctccgtcag cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc
    99901 atgagtgccg gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc
    99961 gattttggca tcacctgctc caccgccacc aactggattg ccaacatgat cgttggcgca
   100021 acgttcctga ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct
   100081 ctgaacgtac tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg
   100141 ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac
   100201 gattaatctc ctcaggcttc ctcccatcgc ggaggaagcc acctcttgca gtcatctttt
   100261 cttcgctcta tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccagcaggc
   100321 cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta
   100381 cccggagcca aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga
   100441 aagctatgaa atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga
   100501 agaagtggta ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc
   100561 gccacatggc aaagagtgga agtggatgat ggaaagcgtg ctgggcgttc ccgcccgtcg
   100621 tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg
   100681 ccaggagcat cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta
   100741 tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac
   100801 tttcctgatc tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta
   100861 acacggagta agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat
   100921 tttccggccc ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg
   100981 taaaagtcca cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg
   101041 gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc
   101101 gagccagccg cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc
   101161 agtgctggca ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg
   101221 aaagcgatat gcataacctg caaccgtcag tcggcgaggt gaatggcgat cgcggcaact
   101281 ttatgtacag ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg
   101341 atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcacgcacct
   101401 acttctatat gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca
   101461 acgcatggaa caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg
   101521 cgaaggtgca aggcaatcat aatccgtatg tgcaacgcgc ttgccaggcg cgaaagagct
   101581 aacctacact agcgggactc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg
   101641 agacgacgcg gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc
   101701 agccattctc acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc
   101761 atggggccgg ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa
   101821 attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat
   101881 cgcgaatctc cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa
   101941 tttacgatcc agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag
   102001 cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag
   102061 attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgcccagcg
   102121 atggatctgg aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg
   102181 cgcgccagta acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt
   102241 ggcccggaag gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact
   102301 gatatcctgt tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc
   102361 gcgctacaag tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc
   102421 atcgtgatgg accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg
   102481 ctggaagcac agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg
   102541 atcaatggcg aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag
   102601 tggttttcgt tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg
   102661 cgtaaagacc cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc
   102721 gaagagaaag ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa
   102781 ctgtttaccg cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg
   102841 cagctaaaag cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg
   102901 ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa
   102961 accctgactg agcatggcac tcgctactgc atggcgcaaa attacctgcc agccattaaa
   103021 gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt
   103081 attccgcagg ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt
   103141 ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgtt gaaagaaaaa
   103201 gggctgattt ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc
   103261 agcccaacct gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta
   103321 atggatgcca tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg
   103381 ttgagactct gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg
   103441 aaccaggaaa cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct
   103501 gctctccagg atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat
   103561 ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg
   103621 gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt
   103681 atgctcggcg gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat
   103741 tttgcttcca gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg
   103801 gaaacgctcg gcaccgataa acaaccgtct gacgtattgg tggctctggg ttatgcctcc
   103861 tgggaaaaag gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg
   103921 gatctgaata ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg
   103981 attggtgtgg atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct
   104041 tactcgcctt cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg
   104101 gcactgctcg ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca
   104161 tcgaacgttt actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata
   104221 tggacggcac cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg
   104281 gtcgtttcgg tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt
   104341 ccggtctgtt tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct
   104401 ctgcggttat tattctcgaa agctatttcg agcagggata ttaaggcgat ctaaaagcct
   104461 ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg aacgccttat ccagcacaca
   104521 tctggcagcg gctgtaggtc tgataagacg cgttagcgtc gcatcagaca tttattgcct
   104581 ttataggccc tataaacttg cgcatcgggc atggcaacgt cacaaacgcc cttcccccac
   104641 ccgctgctga taactctgct gaaacgttaa catccccacc tgctgcccgg tttgaataac
   104701 atgcggtaac tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat
   104761 caataattca aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa
   104821 cacggcccgt aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa
   104881 tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac
   104941 caaatgcccg gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc
   105001 tccgagcaaa atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt
   105061 catacagtgc aaaccaattt cccgctgctg gatcaaacat cgctggctgg catagatata
   105121 ttcaacagga tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac
   105181 catcgccgcc agcgtggtag atttgccgct ccccgtcgcc cccgtcacca gaatcaggcc
   105241 attctcgctc ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgtgg
   105301 acagtgcgaa ggtaacaacc gtaatgccag agaaatacct tgccgttgcg cgaacgcact
   105361 gccacgcaac cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag
   105421 caatattgcc cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc
   105481 atcaaacggc gcagcttcca ttagcccgcg aatgcgccat cgtgcggacc aggcgctgca
   105541 caggtgtaga tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat
   105601 actaagatcc tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa
   105661 tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca
   105721 gtaaaacaaa acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg
   105781 gtgaaaacta cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa
   105841 caggattaga atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag
   105901 agcatttcga ctggtgtcat accatcgacc gtttgcgcat cgctacccgc ctcaacgatc
   105961 agcgcccggc agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa
   106021 acagtaagtc cgggattcaa ctggcagaac tggacgagct ggcagctgcg gtcgctgaac
   106081 taccgcgttt acgtctgcgc gggctgatgg caatccctgc acctgagtca gaatatgtaa
   106141 ggcagtttga agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc
   106201 cgcatatcga cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag
   106261 gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat
   106321 aaggaattaa aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct
   106381 gtataccatg gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa
   106441 ccccttctca cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt
   106501 tattccggca atgggcccca ttgacagcgc ctcgctgctg gttgcctata ttctcagttt
   106561 tatcaaagcc atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgccgg
   106621 tttactgatt ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc
   106681 gattatgagc tgggtaagcc agggtcgtag cccgattgaa tacgtgctga ttcagctggc
   106741 cgatccgctg ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc
   106801 gccgatgatc ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca
   106861 ggcaaccgga aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt
   106921 aatgatgacg gtctggtttt acggctctat attcagccga aagccagccg tgattctatt
   106981 gtcggtttac atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggtcag
   107041 gccaacagtc atctggtgaa gtttctcggt aagcaatttc gggttgccaa aagccaggtg
   107101 gtgattgaaa aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa
   107161 atcccgccag aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc
   107221 aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga
   107281 tatcgtggcc caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat
   107341 cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg accggtttac cggcaattgc
   107401 cgacgactct ggtctggcgg tagatgtgct tggcggcgcg ccgggcattt actccgcgcg
   107461 ttattccggt gaagacgcga ccgatctaaa gaatctgcaa aaactgctgg aaacactgaa
   107521 agacgtaccg gatgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca
   107581 cgcggaagat ccgactccgc tggtatgcca cggtagctgg ccgggcgtga ttactcgtga
   107641 accagcgggc actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa
   107701 aaccgccgcc gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcatt
   107761 gaaactgctg ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc
   107821 acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag
   107881 gagaagtgcc gcacgacgat tacgttcagc atctgctttg cgatctggac aacgatgtgg
   107941 cttacgctca gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc
   108001 tttccggccc ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag
   108061 cggatgcaga aattactatg gaagcgaacc ctggtacggt agaagccgat cgctttgtcg
   108121 attatcagcg tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaca
   108181 agctgaaacg acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg
   108241 cgagcgggct ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat
   108301 cactggaaga ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt
   108361 cctggtatca actgaccatc gaacctaata cgctgtttgg ttcacgcccg ccggtactgc
   108421 cggacgacga cgcgctgtgg gatatattcg aacaggggca tcagttatta accgcagcgg
   108481 gttatcagca atatgaaact tccgcttacg ccaaatccag ttatcagtgc cagcacaatc
   108541 tcaactactg gcgctttggc gactacatcg gtattggctg cggcgcgcac ggcaaagtga
   108601 ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg tcatccgcgt ggttttatgc
   108661 aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct
   108721 ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg
   108781 ggctttgcga agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca
   108841 ccgaatgtgc ggattactgg cagataacgg aacatggtaa attattctta aactcacttc
   108901 ttgaattatt cctcgctgag taatca
//
LOCUS       DAZUFB010000002        98661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZUFB010000002 DAZUFB010000000
VERSION     DAZUFB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.002"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(83..1495)
                     /gene="pykF"
                     /locus_tag="ACHY3M_000107"
     CDS             complement(83..1495)
                     /gene="pykF"
                     /locus_tag="ACHY3M_000107"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFS4315105.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSITLPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1503..1697)
                     /locus_tag="ACHY3M_000108"
     CDS             complement(1503..1697)
                     /locus_tag="ACHY3M_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315106.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2052..2261
                     /gene="fumD"
                     /locus_tag="ACHY3M_000109"
     CDS             2052..2261
                     /gene="fumD"
                     /locus_tag="ACHY3M_000109"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFS4315107.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2717..3343
                     /gene="ydhY"
                     /locus_tag="ACHY3M_000110"
     CDS             2717..3343
                     /gene="ydhY"
                     /locus_tag="ACHY3M_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFS4315108.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            3364..5466
                     /gene="ydhV"
                     /locus_tag="ACHY3M_000111"
     CDS             3364..5466
                     /gene="ydhV"
                     /locus_tag="ACHY3M_000111"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFS4315109.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            5479..6117
                     /gene="ydhW"
                     /locus_tag="ACHY3M_000112"
     CDS             5479..6117
                     /gene="ydhW"
                     /locus_tag="ACHY3M_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFS4315110.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6181..6849
                     /gene="ydhX"
                     /locus_tag="ACHY3M_000113"
     CDS             6181..6849
                     /gene="ydhX"
                     /locus_tag="ACHY3M_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS4315111.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACSKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKLHLYRRFGQHLIK
                     KENV"
     gene            6846..7631
                     /gene="phsC"
                     /locus_tag="ACHY3M_000114"
     CDS             6846..7631
                     /gene="phsC"
                     /locus_tag="ACHY3M_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFS4315112.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7635..8447
                     /gene="ydhT"
                     /locus_tag="ACHY3M_000115"
     CDS             7635..8447
                     /gene="ydhT"
                     /locus_tag="ACHY3M_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFS4315113.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERAWVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(8459..10063)
                     /gene="ydhS"
                     /locus_tag="ACHY3M_000116"
     CDS             complement(8459..10063)
                     /gene="ydhS"
                     /locus_tag="ACHY3M_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFS4315114.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(10189..10494)
                     /gene="ydhR"
                     /locus_tag="ACHY3M_000117"
     CDS             complement(10189..10494)
                     /gene="ydhR"
                     /locus_tag="ACHY3M_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFS4315115.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(10602..10678)
                     /locus_tag="ACHY3M_000118"
     tRNA            complement(10602..10678)
                     /locus_tag="ACHY3M_000118"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10642..10644),aa:Val,seq:gac)
     gene            complement(10683..10759)
                     /locus_tag="ACHY3M_000119"
     tRNA            complement(10683..10759)
                     /locus_tag="ACHY3M_000119"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10723..10725),aa:Val,seq:gac)
     gene            11067..12323
                     /gene="ydhQ"
                     /locus_tag="ACHY3M_000120"
     CDS             11067..12323
                     /gene="ydhQ"
                     /locus_tag="ACHY3M_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS4315116.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(12364..13737)
                     /gene="mdtK"
                     /locus_tag="ACHY3M_000121"
     CDS             complement(12364..13737)
                     /gene="mdtK"
                     /locus_tag="ACHY3M_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFS4315117.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            13952..14593
                     /gene="ribE"
                     /locus_tag="ACHY3M_000122"
     CDS             13952..14593
                     /gene="ribE"
                     /locus_tag="ACHY3M_000122"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFS4315118.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(14633..15781)
                     /gene="cfa"
                     /locus_tag="ACHY3M_000123"
     CDS             complement(14633..15781)
                     /gene="cfa"
                     /locus_tag="ACHY3M_000123"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFS4315119.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(16072..17283)
                     /gene="punC"
                     /locus_tag="ACHY3M_000124"
     CDS             complement(16072..17283)
                     /gene="punC"
                     /locus_tag="ACHY3M_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFS4315120.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17396..18328
                     /gene="punR"
                     /locus_tag="ACHY3M_000125"
     CDS             17396..18328
                     /gene="punR"
                     /locus_tag="ACHY3M_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFS4315121.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18325..19350)
                     /gene="purR"
                     /locus_tag="ACHY3M_000126"
     CDS             complement(18325..19350)
                     /gene="purR"
                     /locus_tag="ACHY3M_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFS4315122.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19649..19738
                     /gene="cydH"
                     /locus_tag="ACHY3M_000127"
                     /gene_synonym="ynhF"
     CDS             19649..19738
                     /gene="cydH"
                     /locus_tag="ACHY3M_000127"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFS4315123.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            19904..21073
                     /gene="ydhP"
                     /locus_tag="ACHY3M_000128"
     CDS             19904..21073
                     /gene="ydhP"
                     /locus_tag="ACHY3M_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4315124.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(21219..21800)
                     /gene="sodB"
                     /locus_tag="ACHY3M_000129"
     CDS             complement(21219..21800)
                     /gene="sodB"
                     /locus_tag="ACHY3M_000129"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFS4315125.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21928..22755)
                     /gene="mepH"
                     /locus_tag="ACHY3M_000130"
     CDS             complement(21928..22755)
                     /gene="mepH"
                     /locus_tag="ACHY3M_000130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFS4315126.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23090..23437
                     /gene="grxD"
                     /locus_tag="ACHY3M_000131"
     CDS             23090..23437
                     /gene="grxD"
                     /locus_tag="ACHY3M_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFS4315127.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23488..28104)
                     /gene="lhr"
                     /locus_tag="ACHY3M_000132"
     CDS             complement(23488..28104)
                     /gene="lhr"
                     /locus_tag="ACHY3M_000132"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4315128.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAETDQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(28197..28844)
                     /gene="rnt"
                     /locus_tag="ACHY3M_000133"
     CDS             complement(28197..28844)
                     /gene="rnt"
                     /locus_tag="ACHY3M_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFS4315129.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(28947..29354)
                     /gene="gloA"
                     /locus_tag="ACHY3M_000134"
     CDS             complement(28947..29354)
                     /gene="gloA"
                     /locus_tag="ACHY3M_000134"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4315130.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(29435..30532)
                     /gene="nemA"
                     /locus_tag="ACHY3M_000135"
     CDS             complement(29435..30532)
                     /gene="nemA"
                     /locus_tag="ACHY3M_000135"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFS4315131.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(30569..31168)
                     /gene="nemR"
                     /locus_tag="ACHY3M_000136"
     CDS             complement(30569..31168)
                     /gene="nemR"
                     /locus_tag="ACHY3M_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFS4315132.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            31271..31510
                     /gene="ydhL"
                     /locus_tag="ACHY3M_000137"
     CDS             31271..31510
                     /gene="ydhL"
                     /locus_tag="ACHY3M_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFS4315133.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            31559..32413
                     /gene="ydhF"
                     /locus_tag="ACHY3M_000138"
     CDS             31559..32413
                     /gene="ydhF"
                     /locus_tag="ACHY3M_000138"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4315134.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQ"
     gene            32536..33057
                     /gene="sodC"
                     /locus_tag="ACHY3M_000139"
     CDS             32536..33057
                     /gene="sodC"
                     /locus_tag="ACHY3M_000139"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS4315135.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(33058..35070)
                     /gene="ydhK"
                     /locus_tag="ACHY3M_000140"
     CDS             complement(33058..35070)
                     /gene="ydhK"
                     /locus_tag="ACHY3M_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4315136.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNIAEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(35070..35927)
                     /gene="ydhJ"
                     /locus_tag="ACHY3M_000141"
     CDS             complement(35070..35927)
                     /gene="ydhJ"
                     /locus_tag="ACHY3M_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS4315137.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(35930..36166)
                     /gene="ydhI"
                     /locus_tag="ACHY3M_000142"
     CDS             complement(35930..36166)
                     /gene="ydhI"
                     /locus_tag="ACHY3M_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFS4315138.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            36367..36801
                     /gene="slyA"
                     /locus_tag="ACHY3M_000143"
     CDS             36367..36801
                     /gene="slyA"
                     /locus_tag="ACHY3M_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFS4315139.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(36848..37315)
                     /gene="slyB"
                     /locus_tag="ACHY3M_000144"
     CDS             complement(36848..37315)
                     /gene="slyB"
                     /locus_tag="ACHY3M_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFS4315140.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            37589..38698
                     /gene="anmK"
                     /locus_tag="ACHY3M_000145"
     CDS             37589..38698
                     /gene="anmK"
                     /locus_tag="ACHY3M_000145"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFS4315141.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            38796..39125
                     /gene="mliC"
                     /locus_tag="ACHY3M_000146"
     CDS             38796..39125
                     /gene="mliC"
                     /locus_tag="ACHY3M_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFS4315142.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            39184..39840
                     /gene="pdxH"
                     /locus_tag="ACHY3M_000147"
     CDS             39184..39840
                     /gene="pdxH"
                     /locus_tag="ACHY3M_000147"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFS4315143.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            39969..41243
                     /gene="tyrS"
                     /locus_tag="ACHY3M_000148"
     CDS             39969..41243
                     /gene="tyrS"
                     /locus_tag="ACHY3M_000148"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4315144.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            41302..42165
                     /gene="pdxY"
                     /locus_tag="ACHY3M_000149"
     CDS             41302..42165
                     /gene="pdxY"
                     /locus_tag="ACHY3M_000149"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFS4315145.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(42209..42814)
                     /gene="gstA"
                     /locus_tag="ACHY3M_000150"
     CDS             complement(42209..42814)
                     /gene="gstA"
                     /locus_tag="ACHY3M_000150"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFS4315146.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(42919..44421)
                     /gene="dtpA"
                     /locus_tag="ACHY3M_000151"
     CDS             complement(42919..44421)
                     /gene="dtpA"
                     /locus_tag="ACHY3M_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFS4315147.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(45032..45667)
                     /gene="nth"
                     /locus_tag="ACHY3M_000152"
     CDS             complement(45032..45667)
                     /gene="nth"
                     /locus_tag="ACHY3M_000152"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4315148.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(45667..46362)
                     /gene="rsxE"
                     /locus_tag="ACHY3M_000153"
     CDS             complement(45667..46362)
                     /gene="rsxE"
                     /locus_tag="ACHY3M_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFS4315149.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(46366..46986)
                     /gene="rsxG"
                     /locus_tag="ACHY3M_000154"
     CDS             complement(46366..46986)
                     /gene="rsxG"
                     /locus_tag="ACHY3M_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFS4315150.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(46990..48048)
                     /gene="rsxD"
                     /locus_tag="ACHY3M_000155"
     CDS             complement(46990..48048)
                     /gene="rsxD"
                     /locus_tag="ACHY3M_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFS4315151.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(48049..49983)
                     /gene="rsxC"
                     /locus_tag="ACHY3M_000156"
     CDS             complement(48049..49983)
                     /gene="rsxC"
                     /locus_tag="ACHY3M_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS4315152.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            complement(49976..50554)
                     /gene="rsxB"
                     /locus_tag="ACHY3M_000157"
     CDS             complement(49976..50554)
                     /gene="rsxB"
                     /locus_tag="ACHY3M_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFS4315153.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(50554..51135)
                     /gene="rsxA"
                     /locus_tag="ACHY3M_000158"
     CDS             complement(50554..51135)
                     /gene="rsxA"
                     /locus_tag="ACHY3M_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFS4315154.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(51212..51652)
                     /gene="ydgK"
                     /locus_tag="ACHY3M_000159"
     CDS             complement(51212..51652)
                     /gene="ydgK"
                     /locus_tag="ACHY3M_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFS4315155.1"
                     /translation="MITTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(51738..51953)
                     /gene="ydgT"
                     /locus_tag="ACHY3M_000160"
     CDS             complement(51738..51953)
                     /gene="ydgT"
                     /locus_tag="ACHY3M_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFS4315156.1"
                     /translation="MTVQDYLLKFRKISSLKSLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(52226..52351)
                     /gene="blr"
                     /locus_tag="ACHY3M_000161"
     CDS             complement(52226..52351)
                     /gene="blr"
                     /locus_tag="ACHY3M_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFS4315157.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            52594..53634
                     /gene="ydgJ"
                     /locus_tag="ACHY3M_000162"
     CDS             52594..53634
                     /gene="ydgJ"
                     /locus_tag="ACHY3M_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4315158.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(53669..54670)
                     /gene="add"
                     /locus_tag="ACHY3M_000163"
     CDS             complement(53669..54670)
                     /gene="add"
                     /locus_tag="ACHY3M_000163"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4315159.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(54774..55946)
                     /gene="malY"
                     /locus_tag="ACHY3M_000164"
     CDS             complement(54774..55946)
                     /gene="malY"
                     /locus_tag="ACHY3M_000164"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFS4315160.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(55956..57548)
                     /gene="malX"
                     /locus_tag="ACHY3M_000165"
     CDS             complement(55956..57548)
                     /gene="malX"
                     /locus_tag="ACHY3M_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFS4315161.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            57723..58751
                     /gene="malI"
                     /locus_tag="ACHY3M_000166"
     CDS             57723..58751
                     /gene="malI"
                     /locus_tag="ACHY3M_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFS4315162.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            58863..59630
                     /gene="hdhA"
                     /locus_tag="ACHY3M_000167"
     CDS             58863..59630
                     /gene="hdhA"
                     /locus_tag="ACHY3M_000167"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFS4315163.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            59851..60441
                     /gene="uidR"
                     /locus_tag="ACHY3M_000168"
     CDS             59851..60441
                     /gene="uidR"
                     /locus_tag="ACHY3M_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFS4315164.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            60830..62641
                     /gene="uidA"
                     /locus_tag="ACHY3M_000169"
     CDS             60830..62641
                     /gene="uidA"
                     /locus_tag="ACHY3M_000169"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4315165.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            62638..64004
                     /gene="uidB"
                     /locus_tag="ACHY3M_000170"
                     /pseudo
     CDS             62638..64004
                     /gene="uidB"
                     /locus_tag="ACHY3M_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            64043..65308
                     /gene="uidC"
                     /locus_tag="ACHY3M_000171"
     CDS             64043..65308
                     /gene="uidC"
                     /locus_tag="ACHY3M_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFS4315166.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTWWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(65353..66861)
                     /gene="ydgA"
                     /locus_tag="ACHY3M_000172"
     CDS             complement(65353..66861)
                     /gene="ydgA"
                     /locus_tag="ACHY3M_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS4315167.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(66962..68137)
                     /gene="manA"
                     /locus_tag="ACHY3M_000173"
     CDS             complement(66962..68137)
                     /gene="manA"
                     /locus_tag="ACHY3M_000173"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFS4315168.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            68336..69982
                     /gene="fumB"
                     /locus_tag="ACHY3M_000174"
     CDS             68336..69982
                     /gene="fumB"
                     /locus_tag="ACHY3M_000174"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS4315169.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            70125..71528
                     /gene="fumC"
                     /locus_tag="ACHY3M_000175"
     CDS             70125..71528
                     /gene="fumC"
                     /locus_tag="ACHY3M_000175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFS4315170.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(71525..72454)
                     /gene="tus"
                     /locus_tag="ACHY3M_000176"
     CDS             complement(71525..72454)
                     /gene="tus"
                     /locus_tag="ACHY3M_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFS4315171.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(72530..73831)
                     /gene="rstB"
                     /locus_tag="ACHY3M_000177"
     CDS             complement(72530..73831)
                     /gene="rstB"
                     /locus_tag="ACHY3M_000177"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFS4315172.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(73835..74554)
                     /gene="rstA"
                     /locus_tag="ACHY3M_000178"
     CDS             complement(73835..74554)
                     /gene="rstA"
                     /locus_tag="ACHY3M_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFS4315173.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            74683..75018
                     /gene="ydgC"
                     /locus_tag="ACHY3M_000179"
     CDS             74683..75018
                     /gene="ydgC"
                     /locus_tag="ACHY3M_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFS4315174.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(75015..75737)
                     /gene="folM"
                     /locus_tag="ACHY3M_000180"
     CDS             complement(75015..75737)
                     /gene="folM"
                     /locus_tag="ACHY3M_000180"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFS4315175.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(75774..77156)
                     /gene="ydgI"
                     /locus_tag="ACHY3M_000181"
     CDS             complement(75774..77156)
                     /gene="ydgI"
                     /locus_tag="ACHY3M_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4315176.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(77342..78286)
                     /gene="ydgH"
                     /locus_tag="ACHY3M_000182"
     CDS             complement(77342..78286)
                     /gene="ydgH"
                     /locus_tag="ACHY3M_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFS4315177.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            78810..80342
                     /gene="pntA"
                     /locus_tag="ACHY3M_000183"
     CDS             78810..80342
                     /gene="pntA"
                     /locus_tag="ACHY3M_000183"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFS4315178.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            80353..81741
                     /gene="pntB"
                     /locus_tag="ACHY3M_000184"
     CDS             80353..81741
                     /gene="pntB"
                     /locus_tag="ACHY3M_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFS4315179.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(81766..82800)
                     /gene="tqsA"
                     /locus_tag="ACHY3M_000185"
     CDS             complement(81766..82800)
                     /gene="tqsA"
                     /locus_tag="ACHY3M_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFS4315180.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVMLSDLNKE"
     gene            83212..83577
                     /gene="mdtJ"
                     /locus_tag="ACHY3M_000186"
     CDS             83212..83577
                     /gene="mdtJ"
                     /locus_tag="ACHY3M_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFS4315181.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            83564..83893
                     /gene="mdtI"
                     /locus_tag="ACHY3M_000187"
     CDS             83564..83893
                     /gene="mdtI"
                     /locus_tag="ACHY3M_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFS4315182.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(83932..84753)
                     /gene="ydgD"
                     /locus_tag="ACHY3M_000188"
     CDS             complement(83932..84753)
                     /gene="ydgD"
                     /locus_tag="ACHY3M_000188"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFS4315183.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(84853..84936)
                     /locus_tag="ACHY3M_000189"
     CDS             complement(84853..84936)
                     /locus_tag="ACHY3M_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315184.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(85029..85337)
                     /gene="asr"
                     /locus_tag="ACHY3M_000190"
     CDS             complement(85029..85337)
                     /gene="asr"
                     /locus_tag="ACHY3M_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFS4315185.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            85608..85712
                     /locus_tag="ACHY3M_000191"
     CDS             85608..85712
                     /locus_tag="ACHY3M_000191"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFS4315186.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(85761..87014)
                     /gene="ynfM"
                     /locus_tag="ACHY3M_000192"
     CDS             complement(85761..87014)
                     /gene="ynfM"
                     /locus_tag="ACHY3M_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4315187.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            87121..88014
                     /gene="ynfL"
                     /locus_tag="ACHY3M_000193"
     CDS             87121..88014
                     /gene="ynfL"
                     /locus_tag="ACHY3M_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4315188.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            88149..89369
                     /gene="mlc"
                     /locus_tag="ACHY3M_000194"
                     /gene_synonym="dgsA"
     CDS             88149..89369
                     /gene="mlc"
                     /locus_tag="ACHY3M_000194"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFS4315189.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            89494..90189
                     /gene="bioD"
                     /locus_tag="ACHY3M_000195"
     CDS             89494..90189
                     /gene="bioD"
                     /locus_tag="ACHY3M_000195"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS4315190.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(90142..91434)
                     /gene="clcB"
                     /locus_tag="ACHY3M_000196"
     CDS             complement(90142..91434)
                     /gene="clcB"
                     /locus_tag="ACHY3M_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFS4315191.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TREYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(91593..92207)
                     /gene="dmsD"
                     /locus_tag="ACHY3M_000197"
     CDS             complement(91593..92207)
                     /gene="dmsD"
                     /locus_tag="ACHY3M_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFS4315192.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(92250..93104)
                     /gene="ynfH"
                     /locus_tag="ACHY3M_000198"
     CDS             complement(92250..93104)
                     /gene="ynfH"
                     /locus_tag="ACHY3M_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFS4315193.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(93106..93723)
                     /gene="ynfG"
                     /locus_tag="ACHY3M_000199"
     CDS             complement(93106..93723)
                     /gene="ynfG"
                     /locus_tag="ACHY3M_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFS4315194.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(93734..96157)
                     /gene="ynfF"
                     /locus_tag="ACHY3M_000200"
     CDS             complement(93734..96157)
                     /gene="ynfF"
                     /locus_tag="ACHY3M_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFS4315195.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(96218..98644)
                     /gene="ynfE"
                     /locus_tag="ACHY3M_000201"
     CDS             complement(96218..98644)
                     /gene="ynfE"
                     /locus_tag="ACHY3M_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFS4315196.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT        24856 a        24273 c        25546 g        23986 t
ORIGIN      
        1 tttattgctt ctggttatcg attaaataaa aaaagcgccc atcagggcgc ttcgatatac
       61 aaattaattc acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc
      121 tcggtaccag tgcaccagaa accataacta caacgtcacc tttgtgtgcc agaccgctct
      181 gcagagccag ttctttaccc agacggtaga aatcatcagt agaagtgatc tctttaacca
      241 gctgcggcac aacgcctttg ctcagtacca actgatgagc cgttttttcg ttggtggtca
      301 gtgccaggat ggtggcatcc gggaagtatt tacgtactgc gcgagcagat ttaccgccct
      361 gagtagcaac cacgatcagc ggagcatcca gtttttcagc agtttcaacg gcaccacggc
      421 ataccgcttc ggtaatgcgc agtttacggt tgtcattgtt gaactcgaga cggctgttca
      481 tcacgcggtc ggtacgttcg caaatggtcg ccatgataga aacagcttcc agcgggtatt
      541 taccttttgc ggattcacca gacagcatca ctgcatcagt accgtcgagg atggcgtttg
      601 caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa tccagcatct
      661 gggtcgcagt gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg
      721 cgaagataac ttcttctacc gggatttcta cacccaggtc gccacgcgca accatgatgc
      781 cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg
      841 agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt
      901 cagaacgctt acgaataaag gaagcagcaa caaagtctac gccttgttcg caaccaaaga
      961 tcaggtcctg tttgtctttt tcagccagtg ctggcagagt aatggaaacg ccaggcaggt
     1021 tcacaccttt gttttcgccc aggtcaccgt tgttcagcac tttacagata actttgttac
     1081 cttcaatggc ggtaacttcc ataccgatca gaccatcgtc aaccagtacg gtgttgccaa
     1141 ctgacaggtc agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa
     1201 cagatttatc agtggtgaaa gtaaaggtct gaccagcttt cagagaaacg tcgttaccgc
     1261 cttccagttt catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt
     1321 taccagtttt gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt
     1381 caccatgaga gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca
     1441 tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt ttcatgacag
     1501 tcttagtctt taagttgaga aggatgggag aaacttgctt tctgggcgca ctgccggaaa
     1561 gcgaatccgg tattacgaaa gtggtggtgc ctcgctctaa ggataggtga catcgaaaaa
     1621 gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg agtgcctgtt tttgagtcgt
     1681 tgagttctac agtccaaagt gctgaaacca ttcaagagtc aattggcgcg cattatacgc
     1741 tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa cttgttagac
     1801 aataaattgt tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa acttcgctgt
     1861 ttgcgcaacg tctggtgcca aagttgtcag tgtgaggata agtgaaagtt catccggtgt
     1921 ataaatatat catcgctcaa ttgctatagt taaaataatt tatcgcgtta tttgtctgat
     1981 tttgtttgat ttttgtttaa ttggttagat tgatatatgc tgatagttgg catgataaat
     2041 aggtataaag aatgggaaat cggacaaaag aggatgaact gtaccgagaa atgtgcagag
     2101 ttgtcggtaa agttgtgctg gaaatgcgcg atctggggca ggaaccaaag catattgtta
     2161 tcgcaggcgt attacgtaca gcattagcca ataaacgcat tcagcgcagt gagctggaaa
     2221 agcaggcgat ggaaaccgtc attaacgcac tggtaaaatg accattcagg atgaggcggt
     2281 aagcttcatc ctgatattct tgcttcagtt gcggtccagt taatcattct aaataacaac
     2341 tgctgaaatt atacgaatcg cgatatgtat tttaatttat aacgataata ttatttttat
     2401 tttctgcaat gaacgtcaca aataattgtc gcttaagtta aatttatttc aaatgatgat
     2461 aaatatcagt tcacttataa tttcttgctt tgtaatttta attgataacg atcaatgtta
     2521 agggctcaga ataatcacat tattttttct cctaaccata aaggattagt ttatcggcgt
     2581 taaatctttt ctcatacact atcgggttag ggaaaatatc tggtgttggg gacagagtca
     2641 gcagcgtatt agttgaatgc ttaatatcag gctgctgaat tgtcagcact gtgtttgcga
     2701 agaggatgcg aaataaatga acccggttga tcgtccacta ttagatattg gcttaacgcg
     2761 attagaattc ttgcgtatat caggaaaagg ccttgctggt ctaacaattg cccctgcgtt
     2821 actgtcactt ttgggttgca aacaggaaga tattgatagc ggcacagtag ggttgataaa
     2881 tacacccaaa ggggtgctgg tgacccagcg agcgcgatgc acgggttgcc accgctgtga
     2941 aatctcttgt accaacttca acgatggctc agtagggaca ttcttctccc gtatcaaaat
     3001 ccaccgcaat tatttctttg gcgacaacgg ggttggctct ggcggcggcc tgtatggcga
     3061 tctcaactat accgcggaca cctgccgtca atgcaaagag ccgcaatgca tgaacgtctg
     3121 cccgattggt gcgattacct ggcagcagaa agaaggctgt attaccgtcg atcataaacg
     3181 ctgtattggc tgtagcgcct gtactacggc atgcccgtgg atgatggcca ccgtaaatac
     3241 cgaaagtaaa aaatcctcga aatgtgtgtt atgcggtgaa tgcgcaaacg cctgcccaac
     3301 aggggcgtta aaaattatcg agtggaaaga tattactgtg tgaatcttgc aaaggaaaaa
     3361 atcatggcta acggttggac aggtaatata ttaagagtca atctcacgac aggaaatatt
     3421 accctcgaag attccagtaa gtttaaaagt tttgtcggtg gcatgggctt cggctacaaa
     3481 attatgtatg acgaagtacc gccaggcacg aaacctttcg atgaagcgaa taaattagtc
     3541 tttgctaccg gcccattaac tggatctggt gccccctgta gttctcgcgt aaatatcacc
     3601 tcactttcta cttttaccaa aggaaattta gttgtcgatg cccatatggg tggctttttt
     3661 gcagcgcaaa tgaaattcgc tggatacgac gtcattatta tcgaagggaa ggcgaaatca
     3721 ccggtatggc tgaagattaa agatgacaaa gttagcctgg aaaaagccga tttcttatgg
     3781 ggaaaaggga cgcgcgcaac gacggaagaa atttgtcgat tgaccagtcc ggaaacctgt
     3841 gtggcggcta ttggtcaggc tggggaaaac cttgttcctc tctctggcat gttgaatagc
     3901 cgtaaccaca gcggcggtgc gggaactggc gcaattatgg gttcgaaaaa cctgaaagcg
     3961 attgcggttg aagggacgaa aggggtcaac attgccgatc gtcaggagat gaagcgtctc
     4021 aacgattaca tgatgactga acttattggt gcgaataaca accatgtcgt gccaagtacg
     4081 ccgcaatcgt gggcagagta ttcagatccc aagtcacgtt ggacagcacg taaagggctg
     4141 ttttggggcg cggctgaagg tggtccgatt gaaacggggg aaattccgcc aggcaatcag
     4201 aacacagtcg gctttcgtac ctataaatcc gtttttgact taggtccggc ggcagagaaa
     4261 tacacagtaa aaatgagtgg ctgccactct tgcccgatcc gttgtatgac ccaaatgaat
     4321 attcctcggg tgaaagagtt tggcgtgccc agcacaggtg gtaacacttg tgtagcaaac
     4381 tttgtccata ccaccatctt cccgaacggg ccgaaagatt ttgaagataa agacgatggt
     4441 cgtgtgattg gtaacctggt tggtctgaat ttgttcgatg actacggcct atggtgtaac
     4501 tacgggcagc tgcatcgcga ctttacttat tgttacagca aaggggtgtt caagcgtgtt
     4561 ctgccagctg aagagtatgc ggaaattcac tgggatcaac tggaagcggg tgacgttaat
     4621 ttcattaaag atttttacta ccgtctggcg catcgtgtgg gtgagctgag tcacctggct
     4681 gatggttcat atgccatcgc agaacgctgg aatttgggtg aagagtactg gggctacgca
     4741 aaaaataaac tctggtcgcc gtttggctat ccggttcacc atgccaatga agcgtcagcg
     4801 caggtcggtt ccattgttaa ctgtatgttc aaccgtgact gcatgacgca tacccatatc
     4861 aattttattg gttcgggttt gccattgaaa ctgcagcgtg aagtggcgaa agagcttttt
     4921 ggttctgaag atgcctacga tgaaaccaaa aactacacgc caatcaatga cgcaaaaatt
     4981 aaatatgcaa agtggtcgct gttgcgggtc tgtttgcata acgccgtcac cctgtgcaac
     5041 tgggtctggc caatgaccgt ttcgccgctg aaaagccgta attaccgggg cgatctggcg
     5101 cttgaagcca aattcttcaa agcgatcacc ggcgaagaga tgactcagga aaaattagat
     5161 ttagctgcag agcgtatctt tacgttgcat cgtgcctaca cggtaaaact gatgcaaacc
     5221 aaagatatgc gtaatgaaca cgatcttatc tgttcctggg tattcgacaa ggatccgcag
     5281 atcccggtct ttactgaagg tactgacaaa atggatcgtg acgatatgca tgcttcgctc
     5341 acgatgttct acaaagaaat gggctgggac ccacagcttg gttgtccgac ccgcgaaaca
     5401 ttgcagcgtc tggggctgga agatattgcc gccgatctgg cagcacacaa tctactgcct
     5461 gtgtaaggaa tgggtgagat gaaccatcgg gatgaattgc cgttagcgaa agtcagcgaa
     5521 gttgacgagg cgaagcggca atggttacag gggatgcgtc accccgttga tacggtaacc
     5581 gaaccagaac cggctgaaat cctggcagag ttcattcgcc agcattctgc ggctgggcag
     5641 ctggtggcgc gcgccgtgtt tctctcgcca ccctattcgg ttgcagaaga ggagctgtca
     5701 gttttgctgg aaagcattaa gcagaacgga gactacgcgg atatcgcttg tatgacggga
     5761 agccaggatg attactatta ctcaacgcag gccatgagtg aaaactacgc ggcgatgtct
     5821 ttgcaagttg tggagcagga catttgtcgc gccattgcac acgccgttcg ttttgagtgc
     5881 cagacatatc ctcgtcctta caaagtcgcc atgctgatgc aagcgccata ttacttccag
     5941 gaagcgcaga ttgaagccgc gatcgccgcg atggatgttg cacctgaata tgccgatata
     6001 cggcaggtgg agtcatcgac ggcggtgttg tacttgttta gcgagcgatt tatgacctat
     6061 ggcaaggctt atggcttatg cgaatggttc gaggtagagc agtttcagaa cccctgaaat
     6121 ttcagggaga tgtcatggat agggtggaca gttgcagcca ctgcattagg ggataatcag
     6181 atgtccttca ctcgacgcaa atttgttctg gggatgggaa cagtaatctt ttttactggt
     6241 tctgcgtctt ctctgttagc gaacacgagg caagaaaagg aagttcggta tgccatgatt
     6301 catgacgagt cacggtgcaa tggctgtaat atttgcgccc gcgcatgcag taaaactaac
     6361 cacgtccccg ctcagggaag ccgtttatcg atagcacaca ttccagtcac cgataacgac
     6421 aacgaaacgc agtatcactt ttttcgccag tcatgtcagc actgcgaaga tgctccgtgc
     6481 atcgacgtat gcccgacagg ggcgtcgtgg cgtgatgagc aggggatcgt gcgggtagaa
     6541 aaatcgcagt gcatcggctg cagttattgc attggcgctt gtccgtacca ggttcgctac
     6601 cttaaccctg tcactaaagt ggcagataaa tgtgatttct gcgctgagtc ccgactggca
     6661 aaaggctttc cgcccatttg cgtcagcgcc tgcccggaac acgcattgat ctttggtcgc
     6721 gaagatagcc cagaaattca ggcgtggttg cagcaaaata aatactatca atatcagcta
     6781 cctggtgccg ggaaactgca tctgtatcgc cggttcggtc aacatttgat taaaaaggaa
     6841 aatgtatgaa cccgtcgcaa catgctgaac agtttcagag ccagttagcg aactatgtgc
     6901 cacagttcac tcccgaattt tggccggtgt ggttgatcat tgccggagta ttgctggttg
     6961 ggatgtggtt ggtgctgggg ctgcatgcct tgcttcgtgc tcgtggcgtg aagaaatcag
     7021 tcaccgatta tggtgagaag atttatcttt actgcaaagc ggtcagatta tggcactggt
     7081 cgaatgcgtt actctttgta ttgttgctgg caagtgggct gataaatcac tttgcgctgg
     7141 tgggcgcaac tgcggttaaa agtctggttg cggtgcatga agtttgcgga tttttgttac
     7201 tcgcatgctg gctcggcttt gtgctgatca atgccgttgg gggtaatggt caccactatc
     7261 gcattcgtcg tcaggggtgg ctggagcgag cggcaaaaca aacgcgattt tatttgtttg
     7321 gcattatgca gggggaagaa catcctttcc cggcaacaac ccagtctaaa tttaatccct
     7381 tacagcaggt cgcctatgtt ggtgttatgt atggattgct gccgttgtta ctattgacgg
     7441 ggctgctgtg tctctatccg caagccgtgg gagatgtgtt tcctggcgta agatactggt
     7501 tattgcaggc gcattttgct ctggcattta taagcctctt ttttatcttc ggtcatcttt
     7561 atctttgcac cacggggcgt acgccacacg aaacctttaa aagcatggtc gatggctatc
     7621 accggcacta acgaatgatc attacacgag cagacctgcg agaatggcgt atcggagcgg
     7681 tgatgtatcg ctggttcctg cgccatttcc cacgcggcgg tagttatgcc gatattcatc
     7741 atgcactgat tgaggaagga tatacggact gggcggagag tctggttgag tatgcgtgga
     7801 aaaaatggct ggcggatgaa aacttcgcgc atcaggaagt ttcctctatg caaaaactgg
     7861 ccacagaccc cggagagata ccattttgca gtcagtttgc ccgcagcgac gatcatgctc
     7921 gcatcggctg ttgtgaggat aacgcccgaa tcgcaaccgc tgggtatgcc gcgcaaattg
     7981 ccagtatggg ttatagcgtg cggattggaa gtgtcggctt taacagtcac attggcagct
     8041 ccggtgaacg cgcatgggtt gctgtaaccg gtaattcttc gcggataagc agcgctggag
     8101 atagtagtcg gattgcgaat accggaatgc gagtacgcgt ttgtacattg ggtgaacggt
     8161 gtcatgtcgc cagtaatggt gacctggtgc aaatcgccag ctttggcgca aacgccagaa
     8221 ttgccaacag cggcgacaat gtacacatca ttgccagcgg tgaaaattca acggtagtga
     8281 gcacgggcgt ggtcgactca attattctcg ggccaggggg aagcgccgca ctggcatatc
     8341 atgacggcga aagagttcgc ttcgccgtcg ccattgaagg tgaaaacaat attcgaaccg
     8401 gagtccgcta tcgtcttaat gaacagcatc agtttgttga gtgctgatag ctttaccttt
     8461 aatcaaacga aagacgccga cgagcacggg atgcaggctt tacgaccgcc cgagccatcg
     8521 cctcaccaat ttctgcacat gccgtaagtc cctgaacgaa aggctggtcg tgcatcaacc
     8581 agggcaacgc gccgaacgct acgcgtccac gaatatcttc gggttgctgt aacgtataat
     8641 cttcgccaat atcagggatt tcatcccctg ttttctgtaa ttgctcgcgt agcccaggga
     8701 aaggaatatc tttcacttta agcggacgct gtccgcgggc atcaataaaa acgtcaaacg
     8761 agtagctgtt gtcttccgtt ttcaggacgg tgcgcgactc attaatttcc attttgtagt
     8821 cttcaccgag ggcgagaata tgaatgattc ccgcttcacg taaggcaagt aggcgacgaa
     8881 tagactctga agggattgcc gcataattat cgatgaaaac cctggcaagg cctttactga
     8941 accgtttatg gtcgtgttca ttcagatgag gaacaatttc ctgtacggct tcatgcaggc
     9001 gcagaatgac ataacgccag ggaacagtat gtttttctcg tttattgcgt tcgacttcct
     9061 ggagattttt ttctgcccag tcaaatgggt cgcgttgttt gcgctcggca aaccaggctt
     9121 gagcaaagga atcgacattc aggctctcta aggctatgcg ttggctccag tctggatcag
     9181 caaacttgat ttcctctact atcaatctaa atacccgatc caaaaggccc tcttcacctt
     9241 tttgaatctc agcatttaat gcctgatcgg tgacgatgtg taagggctcg tagggaatag
     9301 ggcaatagaa atcggcttcg ggtaaaatac ccgtgcgcga catcaacgta atatttagct
     9361 tttcacttgc gttatcgcgg ttaaaaacga cgtgttgttt atcatcttca atgaacgaac
     9421 catgctgaat agccactgcc attgccgcat ccagtccgct caaggatgtt cccataatac
     9481 ccacgttaca cgcatcgacc tttgcttcca tcaggcctga ccacggactg ggaaaatacg
     9541 ttctggttgc ttcttcttca tcaggccaga cgtgacccgt ggcaatcacc gctaaatcaa
     9601 acgtctcgct gggtaaatcc tgatttgtag cgagcatgac gccagcattt gtaatttgca
     9661 gatcggtaac ctggcatgat tcataaacag ccactgcaaa tttttgctgt cgtgcctggt
     9721 ctactaatcg taaaaattga tcgcggaaat attcgcccag cagaattcgc ggtaaaaact
     9781 gacgatcgtg caaggtttct tttttaacgc cataacgctg gagatggctg gcttcttgct
     9841 tttgtagcca ttcgagatac gtacaattaa tcggcggtat ttcaatactg gcaatatttg
     9901 ccagcatcat ttttgagttt tcctcatcac tgtatggcat cccgacaccg gcctcgtcag
     9961 cctgctcgaa gatagaaata gaaagtggag tttgttgctg tagaagcgag aataaggtgt
    10021 agatccccgt aggcccggca cccacaatag caattttttt cattgttact tacctgtacg
    10081 cattctttcg aatttagtgt tatcagataa gtttggttga gatttttaga atttgagaaa
    10141 tttcagacag gtatgaaaag ggcgctaacc gcccttgatt aaattctgtc aggcgagttt
    10201 tgcctgattg atttgactca gtggctcatt gacatcgaaa actttggcga caacttcctc
    10261 aacgccgaga tttttaagtc gggcagtatg tttttccaga taggcaaggg cgcttttttc
    10321 atcagtaaaa agatagatcc caccggcttc gtggttcttt tcactttctg tccatacctt
    10381 ccacagaaaa ccaggttcct gattaatcga ctcagcaagt ggtttaagct gctcagccat
    10441 tgcgtcgcca aatgggccat taaaagcaaa atgaagttgt aacaaagttg ccatagcaat
    10501 tctccttctg ataacgcata cgaagacagt gtaaatcttt taacgtattg gatacagaga
    10561 agaaacagag gaaaaaagga gaagaccaga ttaagaaaat ctggtgcgtc cgagtggact
    10621 cgaaccaccg acccccacca tgtcaaggtg gtgctctaac caactgagct acggacgcag
    10681 gatggtgcgt tcaattggac tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa
    10741 ccaactgagc tatgaacgca acgttgtagg tgacaacggg gacgaatatt agcggcagag
    10801 tgggaaggtg gcaagaggca aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct
    10861 tttaagcaaa ttggctatat tttgttgatt tgcaagggcg atttttattc aggatcgcat
    10921 ttacatctga tacaacccaa taattcgtgt atcagagttt aaacagagtt gtttgccacc
    10981 atctcaaatc tgtcgtggat aaatcctccg tagcctttac acttaggtag caataaaggc
    11041 acacagatgc agcaaaggaa taaatcatgg gatctgatgc gaaaaacttg atgagcgacg
    11101 ggaatgtgca aattgttaag accggcgagg tcattggcgc gacgcaactt actgaaggcg
    11161 agttgattgt tgaagctggc ggaagagccg aaaataccgt ggtcacgggg gctggctggt
    11221 tgaaagtggc aaccggtggg atcgccaaat gcacacagta cggtaacaat ggcacgctat
    11281 cggtcagcga cggtgccatt gccacagata ttgttcagtc cgagggaggc gcaattagtc
    11341 tctctacgct cgctacggtt aatggccgcc atcccgaagg tgaattcagc gttgatcagg
    11401 gttatgcctg cggtttgttg ctggaaaatg gcggtaacct gcgtgtactg gaaggacatc
    11461 gcgcggaaaa aatcattctc gatcaagagg gcggcctgtt ggtcaatggg acaacctcag
    11521 ctgtcgtggt agatgaaggt ggtgaattgt tggtgtatcc aggtggggaa gccagcaatt
    11581 gtgaaattaa tcagggcggc gtttttatgc tggccgggaa agccagtgat acgttgcttg
    11641 ctggtggcac catgaataat ctcggtggtg aagactctga cactattgtt gagaatggat
    11701 ccatttatcg tctggggacg gatggccttc agctctacag ttccggtaag acgcaaaacc
    11761 tgtccgtgaa tgtgggtggt cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa
    11821 tacaaggtgg aacagtgatc ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag
    11881 aggaagatcg cgcaccggtt gaactgaccg ggagtgttgc attactggac ggcgcttcaa
    11941 tgattattgg ctatggtgca gatctgcaac aatcaacgat tactgtacag cagggcggtg
    12001 tgttgattct cgacggcagt acggtaaaag gtgacagtgt cacttttagt gttggtaaca
    12061 tcaatctgaa tggcggaaaa ctgtggctga tcactggtgc ggcaacgcat gtgcaactga
    12121 aagtgaaacg cctgcgcgga gagggagcga tttgcctgca aaccagtgcg aaagaaatct
    12181 cacctgactt catcaatgtg aaaggggaag ttaccgggga tatacgcgtt gagataacag
    12241 atgccagtcg gcaaactctg tgtaacgcac tgaaactaca gccagacgaa gacgggattg
    12301 gcgcaacgct ccagcctgcg taaaaacaca ggcgcaaccc gtgaaggttg cgccttgtct
    12361 ttattagcgg gacgctcgtt gcagaatgat ggctgacggc agacgttgca ggaaccgcat
    12421 acgcagcatc atcataatgg ctgccgacgt caggccaata ataaagccta tccagaagcc
    12481 tgctggcccc ataggttcaa cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa
    12541 gcccagcacc cagtaagccg taaaggtaat atagaaaatg gaacgcgtat ctttataacc
    12601 acgcaaaatc ccactgccaa tcacctggat cgagtcagaa atctgatata ccgccgccag
    12661 caacatcaaa tgcgcagcca gcgttacaac ctcgggattg tcgttgtaca acagggcgat
    12721 ttgctcccgc agtgaaaccg tgaatatggc cgtcagggtt gccatacaga cacccaccat
    12781 aagcccggtc cgcgcagcgg tttgcgcatc cagcgttgag ccctgaccca gacgataacc
    12841 tacgcggata gttaccgctg ccgccagcga catgggaagc acgaacatta gtgaactaaa
    12901 gttcagggca atctggtgtc ctgcgacatc aacaataccg agcggagaca ctaacagagc
    12961 cacgacagca aacagtgtca cttcaaagaa cagtgccagc gcaatcggca aaccgagttg
    13021 aatcagtcgt ttcataaccg caggatcggg tttcgcggtg cctttttcgt tacgaatatc
    13081 gcgcatggag cgggcgcgtt taatgtaaga aaccatggca aggaacataa cccagtacac
    13141 cgccgcagta gccacgccac aaccaacgcc accgagctca ggcataccga aatgaccata
    13201 aataaagata tagttcaccg gaatgttcac cagcaggccg ataaagccca ttaccatacc
    13261 cggcttggtt tttgccagac cttcacactg gttacgggca acctggaaga acagatagcc
    13321 cggcgcgccc cacaacaacg cacgcagata acccacggct ttgtccgcca gagccggatc
    13381 gatgttttcc atggagcgga taatgtaacc tgcattccac agcaccagca taatgagaac
    13441 ggaaacaaaa cctgccagcc agaaaccttg tcgcacctga tgcgcaatgc gctcacgtcg
    13501 accggaacca tttaattgcg cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa
    13561 gaggatcgcc ggaagccaga tagaagtacc gatagcgacc gccgccatgt cggtggcact
    13621 atagccgccc gccatcacgg tatcgacaaa acccatcgca gtttgggcga tttgcgcgag
    13681 aatcaccggg attgctaatg ctaataacag acgcgcttca ctgatatact tctgcacgtg
    13741 aacacctttt atttgtagtt atatgagaga ctaaaaagcc gcctgaatgg gcagcaaaaa
    13801 gaagaagcag gggaaattca gtctattgta gcgaggattt attaattctc cagtgaaaaa
    13861 atagtcattc ttaccccttc actggtaagg gcgtttgcca cctgctattg tgctgaacag
    13921 aatgtcttaa ctgatttcag gagttgtaag tatgtttacg gggattgtac agggcaccgc
    13981 aaaactggtg tcgattgacg agaaaccaaa ttttcgtacg catgtggtgg agttacccga
    14041 ccacatgctg gacggcctgg aaaccggtgc gtccgtggcg cataacggtt gctgcctgac
    14101 cgtgacggaa attaacggca accatgtcag ttttgacctg atgaaagaaa cgttacgcat
    14161 taccaatctt ggcgatttaa aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt
    14221 cagtgatgaa attggcggac acttaatgtc aggtcatatt atgaccactg ctgaagtggc
    14281 gaaaatatta acctcagaaa ataatcgcca gatctggttt aaagtccagg atagtcagtt
    14341 gatgaaatat attctgtaca aaggatttat tggcatcgac ggtattagcc tgaccgtcgg
    14401 cgaagtcacg ccaacgcgtt tttgcgtcca tttaattccg gaaacactgg aacgcacgac
    14461 tcttgggaag aaaaaactcg gcgcacgcgt caacattgaa attgatccac aaactcaggc
    14521 agtggtagat acggtagaac gtgtgctggc ggcacgagaa aatgccatga atcaaccagg
    14581 tacagaagcc tgatctgaac tcgcccccga agagggggcg atagaatagc ctttagcgag
    14641 cgactcgaag gccgttttca acaccgcgtg agaacaccac ctgccagagc tgaatatcac
    14701 gggcgcggaa agcacctgca caagcattca gataataggt aaacattcgt ttaaagcgtt
    14761 cactgtagtt atctgcaatt tctggccatg cggcgaggaa tcgttcatac cacgccatca
    14821 acgtagtatc gtaatcagca ccgaagttat gccagtcttc catcacaaag tggggttcgc
    14881 tggactgagc aatctggcgt acagagggca ggcaaccgtt cggaaaaata tatttattaa
    14941 tccagggatc aacattcaga tcggtttttt tcgaaccgat agtatggagc aggaatatac
    15001 cttccggttt caaattacga tccaccaccg caaaataggt atcgtaattt ttcggtccga
    15061 cgtgctcgaa catccccaca gaaacaatac gatcaaactg gtcgttcagg tcacgataat
    15121 cttgcagcaa aatggtgaca tccaggcctt cacagcgttc ctgagccatt ttttgctgtt
    15181 cggcagaaat ggtgacgccg accacgctta cgtcatagtt agatgccatg tagtgcgcca
    15241 gtccgcccca gccgcagcca atatccagta cgcgcatccc tggttttaac tgcaattttt
    15301 cacaaatcat tttgagcttc gcctgctggg cagattccag attatcggca tctttccagt
    15361 aagcgcagga atattgcatg aagggatcaa gcatgcgact gaacaagtca ttacccaaat
    15421 cgtaatgctc tttgccgact atccaggcac gttttttact ctgcagattg aagagacggg
    15481 cgcctgcaat acgcagcgtg tctttgaaat gatgggggag ttggttctcg agacctgcgc
    15541 gtaagacttt gctaaaaaac atatccagtc ggtcacattc ccaccagcca tccatataac
    15601 tttcgcctaa ccccaaagag ccttcttgca gaacgcgttt aaaaaaatcg gggtttttca
    15661 cacgaatatc cgccggggca gaaccgttaa tggctatacc ggcacggcta agtaattcgt
    15721 tggcgatacg gtaccagttg tcatccggta cactgacttc ttctatacac gatgaactca
    15781 tagtttctcc atcactggtc ggtgatcaga accttaaaac agcgtagacg ctttttgggc
    15841 tttgtgagaa atctcgcaga aaaaaccgcg agtctataat ccgacgtaga acagagggaa
    15901 aggaggaacc cttgccgaaa tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta
    15961 aaaacataat atttatcgta gctttaacaa ctcgaaatta gtataggccg ctaatttcaa
    16021 tcatacaaca aaattttgtt agcagcctaa gtataagtat atcgatatag atcagtgtga
    16081 ttcgctatga gcgacttcgg cattgccatg attctggcag ccaacttctt cacaacgttg
    16141 catcatgtaa cccagcgcga ccagcactac tgttgataac atcacgctgg tggtggtgag
    16201 caatggcgtg ctgatactga tcagccagga aactaccaga cttgcgagga agcacagacc
    16261 cagttgcaga gtgttctgca acgctgcggc gcgaccggtt gcatgtggga agggacgcag
    16321 cgcctgggcg acaacaatag ggtagatcgc gccattggca atcgccatca cacagaatgg
    16381 gatcaggatt tcgaccagcg acacatggct aatgaagccc gcagcccagg tcgcaatgac
    16441 gctgacagca aacagcacca gcaaccacgg taataactgc ttgccttgcc atttctgcag
    16501 tgcggcgcga cagccataac caccaatcag aaacgcgata gtttgcggga cataacttaa
    16561 accaataact gccgggctgt agcccatttc actaaggatg aacggtgaac cggtcagcca
    16621 tgcaaaaaaa ctggctgaac aggctgcata tatcagcacg ttgccgcgat aggttttaga
    16681 acgtagcagg tcggtaaagg tcagaccatc ctgactattg ttacgggcct tcgtcgtggg
    16741 tttgagccag aaaataggca gaatcagcac cacggtaatg gcaaacaggg tggcgaaaat
    16801 cgcctgccag gaaaaatgga ccagcagcca gcttcctaac aggggagcca gtgccggaga
    16861 cagacccacc agcggcatga tggtcgcaaa aatacggttg actttctgtg aaggataata
    16921 atctgtcact aatgcttgcc agataaccgc cgcggcgcag acccccacag cctgtacaaa
    16981 acgcaatacc agcagcgtag cggcgttttc tacccacagc atccccagac tacctaacgc
    17041 aaaaattgtc aggccgatta ataacaccgg tttacgacca taacggtcgg agagtggccc
    17101 ccacagaagc tgggctgcgg caaaaccggc aaggaacaga ctaaggctgg cactgacagc
    17161 agacgcaggc gtttgcaggt cggcctgtat ggcggcgaaa gcaggcagat acatatcggt
    17221 tgccagaaaa ccgagtacgc tcaaacccgc cagccagact aaaaatcttt tcccaggttg
    17281 catgtatatt ctctaaatct ctcaattaaa caccgccgca aagtgtaggg agtgaaatta
    17341 gtgttgtgaa acggtaatat ttgatgactg gtttcaaaaa ttttgcaggc agactatgtg
    17401 gtcagaatat tcactcgaag ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc
    17461 ggcacaggag ctgcatcgcg ttccttctgc ggtcagctat accgtgcgtc agctggaaga
    17521 gtggctggcg gtaccgctct ttgaacgacg gcaccgtgat gtggagctga ccgctgctgg
    17581 agcgtggttt ctcaaagaag ggcgctctgt tgtcaaaaaa atgcagatca cccgccagca
    17641 atgtcagcag atagcgaacg gctggcgcgg tcagttagct atcgcagtgg ataatattgt
    17701 caggccagaa cgtacacggc agatgatcgt tgatttttat cgccattttg acgacgtcga
    17761 acttctggtc tttcaggaag tgtttaacgg tgtctgggat gcgctttccg atgggcgcgt
    17821 ggaactggcg attggcgcaa cacgcgcgat tccggtaggc ggtcgttatg ccttccggga
    17881 tatggggatg ctaagctgga gttgcgttgt tgccagccac cacccgctgg cgttgatgga
    17941 tggcccgttc agcgatgata cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc
    18001 gcgaacgcta cctaaacgta ttacctggtt gctggataac caaaaaagag ttgtcgtgcc
    18061 tgactgggaa tcatcggcaa cctgtatctc ggcaggatta tgcataggga tggtgccaac
    18121 acatttcgcc aaaccgtggc ttaatgaggg gaagtgggtg gcattagaac tggaaaaccc
    18181 ctttccggat tcggcatgtt gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac
    18241 ctggctgctt gagtatctcg gcgatagtga gacgctgaat aaggagtggc tgcgggagcc
    18301 ggaagagact cccgcaacgg gtgattaacg acgatagtcg cggaacgggc cgtcagccac
    18361 ggagcggcgt tcaatcaagc gcggatgcac ttcaatggac tgcggttctt cacgtttgtt
    18421 gacgatacga tccaacagca tgttgaacgc tgtttcaccc agcgaatctt ttggctggtg
    18481 gatcgtggtc agcgccggcg taaaatagcg ggcgttgcgc acgttatcat aaccgatcag
    18541 cgaaacatcc tgcgggacgc gcagacccat ttcatcagca gcacaaagtg cgcccattgc
    18601 catgatatcg ccaccacaga agacggcagt agggcgatgc ggctgcgaca gtatttgctg
    18661 catggcgcga taaccagatt cgggttcaaa gtcaccctga acaatccagc tttccggcac
    18721 cttgatcatc gcttcttcca tcgccttcat aaaaccggca aggcggcctg cgccggtgtt
    18781 acgttccagc gggccgggga tgacgccgat ttcgcggtga ccgcgttcaa tcagataacg
    18841 cccggccatg tagccgcctt cgaacgcgtt atcaatgacc gcatcggtga agtcagcttt
    18901 tgcttcaccc cagtccatca ccaccattgg gatatggcga tactcttcca gcatcgccag
    18961 caacggttct gggtactcag aacacatcac cagcagacca tcgacgcgtt tttgcgccat
    19021 catcgacaga taagcccgct gtttctcaag attgttccac gcattaccca gaatcagggt
    19081 gtaacctttc tggaagcaat ttttttcaac cgcttcaatg atctcggcaa aataggccgc
    19141 ttcgctgctg gtcgccagca aaccgataga cttggtgtgg ttaaccttca ggctacgcgc
    19201 caccgcgcta ggggagtagt gtaattcttt aatcgctgcc cacacggcgt tgcgcgtttc
    19261 ttcagcgacg aaacgtgttt tgttgattac gtgtgacaca gttgtagtgg aaacgtttgc
    19321 tcgtttcgct acatctttta ttgttgccat tccatttcac tccagaccct aacttcagct
    19381 cctgcaaaat cgcaaggtaa acgtttgcct ttacacacct tacgcacaac aaaagtgttg
    19441 cggtacgccg gaaaaacgac acggagcgtc aggaaggggt caatggccag tacgcaaata
    19501 gtgtaagcgt ggaattttgg ccgatcttga cgaaaagggg aagtggtaaa aacacttatc
    19561 attataaatg acgggagatt ttttcatcac agtgtgtaaa aatgcgtaat atcactattt
    19621 gtgtggtaat aaaaaaggag aaaacttgat gagcaccgac cttaaatttt cactggtaac
    19681 aacgattatc gtcctcggtt tgatcgtggc cgtgggtttg actgccgcgc tgcactgatt
    19741 tcctctcgag ggagcgcgtt ctccctcgca tctccttcct gtttgttagc aaaatggcaa
    19801 aagtgttttc tctttttgtt attttcattt ttttgtgatt gctgtcatgc tttcgccact
    19861 ttaatgaata tcgcgtaatg acgcgaagag gtggagataa ggcatgaaaa ttaactatcc
    19921 gttgctggcg ctggcgattg gcgcgtttgg tatcgggaca acggagttct cgccaatggg
    19981 tttgttgccc gtcattgcgc gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat
    20041 cagtgcctat gcagttggcg taatggttgg cgcgccgctg atgacgcttc tactttctca
    20101 tcgtgcccgc cgcagtgcgt tgattttcct gatggcaatt ttcacgctcg gcaacgtact
    20161 ttccgccatc gcgccggatt atatgaccct gatgctttca cgcattttga ccagcctgaa
    20221 tcacggagca ttttttggtt tgggttcagt cgtggccgca agcgtggtgc caaaacataa
    20281 acaggccagc gcagttgcca ctatgtttat ggggttaacc ctggcaaata tcggtggcgt
    20341 gccggcggcg acctggttgg gtgaaaccat cggctggcgg atgtcatttc tggcaacggc
    20401 ggggctggga gtgatttcaa tggtaagtct gttcttctca ttacctaaag gtggtgcagg
    20461 ggcacgacct gaagtgaaaa aagagctggc ggtattaatg cgtccgcagg tgctgtctgc
    20521 attgctgacg acggtactgg gagctggtgc aatgtttact ctctacacct atatctctcc
    20581 ggtactacaa agtattaccc acgcaacacc agtgttcgtc acggcaatgc tggtgctgat
    20641 tggtgtcgga ttctctatcg gtaactatct cggcggcaaa ctggcagatc gttcagttaa
    20701 cggcacgttg aaaggctttt tgttgttgtt gatggtgatt atgctggcaa tcccgttcct
    20761 ggcccgcaat gagttcggcg cagctattag catggtggtg tggggcgcag caacctttgc
    20821 ggtcgtaccg ccgttacaga tgcgcgtgat gcgtgtcgcc agtgaagcgc caggtctgtc
    20881 ttcatcagtc aatattggtg cctttaatct tggaaatgcg ctgggagcag ctgctggtgg
    20941 tgcggtaatt tccgctgggc tgggatacag ctttgtgccg gtgatgggag cgattgtcgc
    21001 gggactggca ttattgctgg tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt
    21061 tgctaacagc taattaaaca aaaacgcaga aggataacct tctgcgttac agttgctgac
    21121 aacgtgcgat ttatcttgcc gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag
    21181 cgaagcgcat caggcaatgc tgcatttgcc atcagttatt atgcagcgag atttttcgct
    21241 acgaattccc agttcaccag cgcccagaag tgctccagat agccaggacg tgcattgcga
    21301 tagtcgatgt aataagcgtg ttcccagaca tcaacggtca gcagcggagt cgcatcggtg
    21361 gtcagcggag tacccgcgtt agaggttgaa acgatagcca gtttgccatc gctgtttttc
    21421 accagccagg tccagccaga accaaagttt ttgatcgctg catcagtaaa ctgcgctttg
    21481 aaatcggcaa agctgccaaa agatgcggcg atagcttcag cgacttttcc agtcggttcg
    21541 ccaccggcgt tcggtgccag gcagttccag tagaaagtat ggttccagac ctgagctgcg
    21601 ttgttgaata cgccaccttc agagctgcga ataatctctt ccagtgattt accttcaaac
    21661 gcggtacctt taatcaggtt gttcaggtta gtgacataag tctgatgatg cttgccgtag
    21721 tgatactcga tagtttccgc agaaatgtgc ggtgccagag catctttagc atatggtagt
    21781 gcaggtaatt cgaatgacat tgctactctc ctttattatt aatttgcata cgtacaatag
    21841 ccttattgtg cgtatggata gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc
    21901 ctgttgccgt aacaacaggg taaagtttta gcgaagtgtt tttggggtca ttacccgacg
    21961 agcgccaaca tagtggcgct gccagtagtc ttcactgaga gaagtgattt gaatttcctg
    22021 acctgtgcgc ggtgactgaa taaatttgcc gttgccgaca tacacgccga catgatcggc
    22081 tgtgccgcgt ccctgagtac ggaaaaagac caggtcgccg tttttcagtt cactacgttc
    22141 gattggcgct gcatcacgca ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt
    22201 caccaaatct ttataagcgt aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga
    22261 gctgccaccc caacgatatg gcttaccaat ttgctgcatc agtttattca ttgccacttt
    22321 cgtagctttt tgcaccttcg ctttgtgcgc gtcggcaaga gttcctgacg cggcattttt
    22381 gactttcaca caatgcgatt tatagccctt acgtttggta cacttttcgg taaagctggc
    22441 tgaggccgtt ttattgacgg cgtgtttgct gcgagttttg gcgtttttag aagcggtaat
    22501 ggaagatttt ttaacggaag aagcggtttt gcttttggtc gtacttttac tggcggcttt
    22561 tttcgctgtt ttctggctgg ctttttgggt ctttttggtg gttgccgtgc ttttcttttt
    22621 atctgctttt ttggtgatat gagtggtagc agaactctcc ctggcttgct ttgaagcatg
    22681 ggccataggc gttaaaggca gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg
    22741 gtttatccgc gccactgagc gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat
    22801 ttacaaaact tgatgattct aatgcataga agaagagaaa gttaatagct tttattgtta
    22861 aaaaatacgt ttcgttggcg agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat
    22921 aaatacgcca gtttgccgag catcagggaa gaaacgaata tttaaaatgc aacaaataga
    22981 gcaaagggat aaaatagcaa aagcgtgatg aaaatgttat tttccgatgc cgcactgacc
    23041 cgctacaatg tcagattact gccgtaaaga agtttgagga agcaagacaa tgagcaccac
    23101 tatcgaaaaa atccaacgcc agattgctga aaacccgatc ctgctgtaca tgaaaggttc
    23161 accgaaactg ccgagctgcg gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg
    23221 cgaacgtttt gcctatgttg atattctgca gaacccggac attcgtgcgg aactgcctaa
    23281 atatgctaac tggccgacct tcccgcaact gtgggttgac ggcgagctgg taggcggttg
    23341 tgatatcgtg atcgaaatgt atcagcgtgg cgaactgcag cagctgatca aagaaactgc
    23401 cgctaaatac aagtctgaag agccggacgc ggaataatcc gtcctgcaat actgacggat
    23461 gcgaacgggc atccgtcagt cctttctcta tccccaatcc agcccttgtg gcgaacttga
    23521 aaatcccgcc tcacgcagca gcgtaaacat cggcgtttgc cggaccggta gatcattcac
    23581 ttctgttagc gtaaagcgca gccgtggttc gcgacgcagt gcggtagtca gcgcgtggaa
    23641 aacctctggg gcgagtaatt cctctttttc ctgccagacc agcatttttt tgccaccttg
    23701 cgccagatag agtaacaatt tgccgccaga aacgaccacc agcgcacccg cccgacgcgt
    23761 tggaaccagc gttgccggat gtgcaggcca gggcagaaga tttccccaca cattagccgg
    23821 atcgttggca gagagcgcca ctggtgtata gtggcgcgtt tgcgtggctt gtgtcgccag
    23881 atcgcgcaat cggtcaatag tcagacgttc agcgaattgc gcgccaccca gaccttctac
    23941 aaaacgacct cgcataattc gcccggagtc ttccatactt cgacaaagcg tttgcatcga
    24001 tggaaacccg ccagggatat tttcggctat caccgcctga cgactgatga tgccgtagcg
    24061 gtcgagcata ttttccgcca gcgccagcat cctttcggta tcgtttagtg gctccacctg
    24121 caataacgac cagcgtccag ccagatttgg tgtgttgtaa gataccagcg gcgagacggg
    24181 ttgcgcatag acaggacgtc cacgacgagc ccggtgactg cggcgagttg aggtgcgtgc
    24241 gttggaactg ctgcgggtga gggcgcgtaa cggtgcccaa atgtcgctgg tgatgacgcc
    24301 ttgccagacc agcgcccata acgcctcttg cagggcagag agatcaaccg attcgccgat
    24361 tttgtcgcgt atccgttggc tgatttgttg tgcaaaccag gctcctccgt cagccagaac
    24421 agcgattatc gcttgctgca gcgccgaacg attcgcctga tccgtttcgg cgggagtgaa
    24481 cgattctgcg gcatattcct gtagatgcag tgccaccagg ccgtcatctt cacccagctt
    24541 tttttgtccc gaccagataa ccgctccggt tgccagtaat tcatcgagca tttctggcga
    24601 atagtcgcgt acgcgggcag gcaggatctg gctttcccag agtgaggcgg gtaaaccgac
    24661 tccggcaagc tgttcgatca cccgcatcac gccatctacg ccctcataaa cgcctggcga
    24721 tgttgaggca aagagcgccg ggctaccatc ggtggcgggt aatacgccct gacgttccag
    24781 cagcaatcgc gcataggtcg tggctgcaac gggacgcgtc gcttctctgg cggcttgcag
    24841 cgagcgcaaa cgcagacgac gaaatacttc atcgctcacc cagatgtctt gttgcagatt
    24901 catcaccaga ccctgttcac gcagttgctg aagctgctct tcgacaatgg caataccgag
    24961 actaaactca tgagccagtt gttcagccgt gaccagagca tgagcccgga gatagcgcag
    25021 aaagaggtcg cgaagcgggt aactgactct atgtaaataa atctctggca atgactctgg
    25081 tagtcgtacg ccgagggcat cacgcagcct ggcggcatca tccatacagg caagacgctc
    25141 ctgtccgcta atcatcgctg ggaagattcg ttttactgca agaagatttt ccagatacga
    25201 cgcaacctct tcactgctgc ctgtatgccg ttgcgccagg tcttcaacgg tcattggccc
    25261 cagttcgcgc agcaggtcga acaatccttc ttcacctttc gctcttctgc caggagccag
    25321 tcgttgcaac tcttcttcca cctggcgaat gacctgcggg tcgagtaatt cccccggatc
    25381 gacctgtccg agtagattgc gcagtaactc gctgtccagc gacagtacgg atgcccggcg
    25441 ctctgccagc ggggcgtcgc tctggtacat aaattccgcg acatagccga acaataaact
    25501 tgtggcgaaa ggcgagggcg tagtggtcgt tacatcggat atttgaattt cgccaccgtt
    25561 caggcgacgc atcaaacgtt ccagtgcggg aagatcataa acatcttgca gacattcgcg
    25621 tagggtttcg agaatgaccg gaaaatccgg atatccctga gcgatttcca gcaactgact
    25681 ggcgcgcagc cgttgttgcc aaagcggggt gcgatggccc ggagtgcgcc ccggcattaa
    25741 taatgcccgc gcggcgcatt cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc
    25801 ctcgcggaca atttgcagca acttttctgg ttcaaacaaa aaaatcgcgg cgtcgggcaa
    25861 tttaccatca gtgtcaggaa tgcgcgcaac aatgccgtca tcactggcga ccacagacgc
    25921 gtcagcgccc catagggcat gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac
    25981 ccgtcttcca tagggagagt gcaaaataat acgccagtcg ccaatttcat cacggcaccg
    26041 ttcgaggacc agatggcgac tccccggaac gatgcccgtc gcgttgcgtt gctcgtcaat
    26101 caacccctga atattggcgt tcgtattttc ttctgccagc cacgggggaa tagtcccgga
    26161 aaagaacgcg ccatccgcca gtaaatgaag aaaatcgccg atcatctcgc ctaattcagc
    26221 cggacgtccg ttaccttcac cacgccagaa ggggagccgg gcagaacgac ccggagcagg
    26281 agtcacaatc acctgatcgc gggtgatttg ctggatccgc catgaggtag cgccgagagt
    26341 gataatgtcg ttcacccgcg actcatatac catctcctca tccagttcac ccacccgccg
    26401 cgaaccggcc tgttcttcac cttcgggtaa taacacgcta tacatgccac gatccggaat
    26461 ggtgccgccg ctggtaaccg ccaacaattg agcgccaggt cgggcggtca atatcccggt
    26521 ctccctgttc cagaccagtt tggggcgaaa agcagaaaaa tcgccagagg gatagcgccc
    26581 ggaaagcata tccagcgtgg cgtcaaaaac acgtcttggc agatctttcc acggtgcggc
    26641 acggcgtacg cgggagtacc attcgtctac ctgtaatgca tccatcgccg cggcggcaac
    26701 ggtttgctgc gcaaggacgt cgagtggatt atgcggcggt gtcaggtttt ccagcctgcc
    26761 tgcgaacata cactctacaa tgactgcgga atcgactaag tcacgccggg tacgagggaa
    26821 aaacagccct ttagatacac cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc
    26881 actggcaaca gaaagcggcg ttgccacctg aatcaccaga tccaccgcgc ccatatcaat
    26941 ccccagttcg agactggagg ttgcgaccac gcagcgtaat tcacccgatt tcagcgcctg
    27001 ttcggtgatt gctcgttgtt ctttagagac ggagccgtgg tgtgagcggg caataaaaac
    27061 gtcgctactt tgtacatggt tagaggttgc gccggaggtc gactcgaaat gggccgtatc
    27121 aacggcgata gacggggaac gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt
    27181 cagtttttcc gccagcccac gtgaattagt aaagacaatg gtcgagcgat ggcgcaacac
    27241 ttcatcaagg atacccgttt caatatatgg ccagatggag ccttcccggc cggcatggct
    27301 gtcttcgccg gtgccgctgg cgaccgatga gacatcatcc atattggcga ccggtacgac
    27361 aattcgtatc tgcggatggc gcattgcggg cgggttgact accgtaaccg ggcgatcgcc
    27421 accaagaaat gctgccacat cgctggctga gcgcacagtg gcagaaaggc caattcgctg
    27481 tgctgaggtg tggagcagcg catcgagccg ctccagactt aacgccagat gcgcaccacg
    27541 tttactgccc gctaccgcgt ggacttcatc aataattacc gtttcgacgc cgcgtagcgt
    27601 ttcgcgcgcg cgggaggtca gcatcagata gagtgattcg ggtgtggtga tcagaatatc
    27661 cggcggatta cgggtgagtt tgctgcattc ctgtgcaggc gtatcgccag tacggatccc
    27721 tacgcgaaga ttgacttccg tttcgccgcg ccgccgccgt tcatcggcaa tacccttcaa
    27781 cgggagctgc aagttgcgct gaacgtcggt gcccagagct tttatcggtg aaatatagag
    27841 gatgcgtgag gttttacgct tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag
    27901 ccgatcgagg gcgtagagaa atgctgccag cgttttcccg gagccggtcg gtgcaatcac
    27961 cagcgcatgt tcgcttcgcg ccgccacatg ccaggtttgc gactggacag cggtcggctg
    28021 tttaaaggcg cgaagaaacc agtcgcgggt cgccggtgaa aacacgtccg gcaggagcga
    28081 tgaagggtca ggattatctg ccattaacgc tattccccgt gaatatcatg cttgatgcgt
    28141 ggaatagcct cactatagat gcctgaatca ttgcatgtca tcaggcatcg actcgattac
    28201 acctcttcgg cggcaggtag cggccagcct cccagacgtt tccagcggtt gacgatttca
    28261 caaaacagca cagcagtgcg ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg
    28321 aagtccatgc cagcggtctg gcaagccttt gacaatacgg tttgtccgag tgccagcccg
    28381 gccagtgcag cagtgtcaaa agtggcgaaa gggtggaacg ggttacgttt cagtgaggcg
    28441 cgttctgcgg cggccatcat aaagctgtga tcaaaattgg cattgtgcgc caccataatg
    28501 gcgcggttac agccgctcgc tttaatacct ttacgtacaa ctttaaaaat ttcgtgcagc
    28561 gcctcgtatt cgctgaccgc gccgcgatcg ggatcgttcg ggtcaatgcc gttgaaggcg
    28621 agggcctctg gctgcaaatt tgcgccgaca aatggttcga cgtggaaatg taatgtggtg
    28681 tccggcatca gccagccttg ttcatccatt ttcagggtga tggcggcaat ctcaagcagc
    28741 gcatcggttt tggcgttaaa tccggctgtt tcaacatcga tcacaacagg ataaaaacca
    28801 cgaaaacggt cgcacagacc ggtaagttga gcgttatcgg acatctgatt ctcttaatac
    28861 ggataaattg cagcgcgcat tatgacaaat attacttgtc gatgcagtaa agatgcgggc
    28921 gcgatgagtt cacgcccggc aggagattag ttgcccagac cgcgaccggc gtctttctct
    28981 tcgattaact caattttgta accgtccgga tcttccacaa acgcgataac cgtagtaccg
    29041 ccttttaccg gacccgcttc acgggtcacg ttacccccgt tttgacggat tttttcgcac
    29101 gcttcagcgg cgttatctac gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat
    29161 ttatccacgc cccagttgta ggtcagttca atcaccgctt cttcggtttc cgggccgtag
    29221 ccaacaaacg ccagtgagta tttgtattcc gggttttcgc tggtacgcag cagtttcatg
    29281 cccagcactt tggtataaaa atcgatggag cgttgcagat cgccaacgcg cagcatggta
    29341 tgaagaagac gcatcttttt atcctcaaaa tggctaacag attcaaaatg ttgttttagt
    29401 atagcggcgg ctttacgccg ctcgcaatgt tggattacaa cgtcgggtaa tcggtatagc
    29461 cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg gttaagctca gctttgcgct
    29521 gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg accaaatgcc accgcatcaa
    29581 ttaacccttt gccgatcagc gtttcagctt tttctactgt gtatgcacct gcgccgataa
    29641 tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc atcagtatac ggttcacccc
    29701 ccgcccaatc tggttctgac atatgcagat aagcaatgcc gcgtttaccc agttgttcaa
    29761 tcagatacag tgcatcggct tcttcattcg cgccgttatc cgtgttctgg aaagtaccga
    29821 ttggcgaaac gcgaatgcca atgcgatcgg caccccattc ttcaatcccg gcatcgacca
    29881 cttccagtac cagacgtgcg cgattttcca cgctgccgcc gtactgatcg gtacgatggt
    29941 ttgaagaagg agaaaggaac tgatgcagca aataaccgtg agcagagtgg agctctacca
    30001 gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg gaaatcattg acgatacctg
    30061 gaatctcttc cagttcaagc gcacgcggca tggatgtttc aacacggatc gcctgaccat
    30121 tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct
    30181 gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt gtgccacagc tgcacggcca
    30241 tatgaccatt ttcagcatga acgccagcgg tgattttttt ccatgcggca atttgctccg
    30301 gactatggat gccaggcgca cctgcatatc cttttgcctg ggcagaaatt tgcgtggctt
    30361 cactaataat caaaccggca ctggcacgtt ggcgatagta ttccgccatc aacggggtag
    30421 gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg
    30481 ccgccgtgat cgcgcccact ttcagtgggg aatacagttt ttcagatgac ataaaggctc
    30541 ccggtcgtct ggtgataaaa aatgcctgct aaacggcagg cgtcgcaata atgtttttta
    30601 catgggccag cgcgttttcc agtggctcga aactgcgcga aattttggcc tgcagattcg
    30661 cacccagcca tagtgcgtaa agcacttgtg cctgttgtag cggttcgcca caaaaggtta
    30721 aacaatggtt ctcacggcca ttttccagcg cctgagagag cagggcgatc acgccgcgag
    30781 cacctttatc catcgcgctg cgcatatctt ctgacagatc gcacacttcg gcagagagtt
    30841 ttactgtcag gcaaccactg atggttccat gttggcaaaa ctggttcagt gtttgctggt
    30901 aataagccag tatgcggtcg cggtagttac cttcgccgga ttgcagcaac tcagtcagtc
    30961 gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac gccaaacgct tctttagagc
    31021 gaaagtagtg atagaaggac cctttcggca cttcagcggt ttttagtaat tcgcttagcc
    31081 ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc
    31141 gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt agtagaccag tcggtctagt
    31201 gcaagcaaag ttgctacttt ggacattaac agcttcaata atggtaataa tcttatctga
    31261 ggagggcgaa gtggcggagc aattagagtt ctttcccgtc cagagcccgt gccggggaat
    31321 ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt cgtagtcgtg atgagcgttt
    31381 taactggaat aaaatgagcg atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg
    31441 attaatgcgc aaattacgcg caaataagcc agcgtcatca gacgaaccgg agcaaccatc
    31501 actcttttga acctaatctt gcgtatactc ctctcatatc tgataagagg aagtgattat
    31561 ggttcagcgt attactattg cgccgcaagg cccggagttt tcccgttttg tgatgggcta
    31621 ctggcgattg atggactgga atatgtccgc ccgccagctg gtcagtttta ttgaagagca
    31681 tctggatctc ggcgtgacca ccgtggacca tgctgatatt tatggtggct atcagtgcga
    31741 agcggcgttt ggcgaggcac tgaaactggc acctcacctg cgtgaacgga tggaaatcgt
    31801 cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac gtcattggtc attacatcac
    31861 tgaccgcgat cacatcatta agagcgccga acagtcgcta attaatctcg cgaccgatca
    31921 tctggatctg ctgttaatcc accgaccaga cccgttaatg gatgccgatg aagtggcgga
    31981 cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat tttggcgtat cgaactttac
    32041 gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt acccttgcca ctaatcaggt
    32101 ggaaatatcc ccggtgcatc agccgttact gctggatggc acgctcgacc aactacaaca
    32161 actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga
    32221 ttatttccag cagctgcgtg atgaactggc tgtggtggca gaggagttaa acgcgggctc
    32281 gattgaacag gtggtttacg cctgggtatt acgtttacca tcgcagccgc tgccaattat
    32341 cggttcaggt aaaattgagc gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac
    32401 ccgtcaacaa tgatttcgta tccgtaaagc ggcactgggg tacgacgtac cgtaagcact
    32461 tttaggaata gccgccgttc aaaaatgtgt cactggttta cacttattca ggaatgcaca
    32521 atgaacggag gtcctatgaa acgttttagt ctggctattc tggcgctggt tgttgcaacc
    32581 ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg
    32641 cagtcaattg gtagcgtcac cattactgaa accgataaag gtctggagtt ttcgcccgat
    32701 ctgaaagcat taccccccgg tgaacatggc ttccatattc atgccaaagg aagctgccag
    32761 ccagccacca aagatggcaa agccagcgcc gcggaatccg caggcgggca tcttgatcca
    32821 caaaataccg gtaaacatga agggccagaa ggtgccgggc atttaggcga tctgcctgca
    32881 ctggtcgtca ataatgacgg caaagctacc gatgccgtca tcgcgcctcg tctgaaatca
    32941 ctggatgaaa tcaaagacaa agcgctgatg gtccacgttg gcggcgataa tatgtccgat
    33001 caacctaaac cgctgggcgg tggcggtgaa cgctatgcct gtggtgtaat taagtaatta
    33061 agaggccagc gtaccttgcg gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg
    33121 ccagacaatt gccgccagct cacgggcggc aggttgatga tgacgggcaa ggctgtcgca
    33181 aatccgctgt aattcttcaa gtgtggccgc cagtgatttt tgctgaacgc cacgctcact
    33241 catcactccc cgcaacagtg aaatacagtt atcccgtact cgcgataacg gatcggaacg
    33301 cgattcccag tcgcgcaatt gccagacaac atgagaacag ttcagcagca ctacacccca
    33361 gcgtaataac caacggcgag ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg
    33421 ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta gctgatcgac
    33481 aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg atcctggacg
    33541 cagaatggcg aacgctagcc aagccagcgc aacgccaacg atttttgcca gattatcgtt
    33601 aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg aacccataaa
    33661 aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat
    33721 tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa tctggaccat
    33781 caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc gcatcagcag
    33841 tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa ttgctgccag
    33901 cgttaatgca ttggcaccgg catcccattg cgatgcaata ctccatgcgc caatcatcat
    33961 caacgtacaa aatgtacgca gcccgctcca catagcttcg gcgttgtcgg tatgacgagc
    34021 cagagcggat gtccgtgcga gtctggcatg atcatctaca ccgctttgca gacgatgtaa
    34081 ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc agaaggcgac
    34141 gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg ataatacgtg cgacggtgta
    34201 agcatctgtt tgcgaactgg cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc
    34261 acctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga cactggtcat
    34321 acgtaattgc tggtggagta gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg
    34381 gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa tcaccccctc
    34441 atgtgctgca cgaatggcat cgtttgtttc aggctgccag agtaaactgg catgttccag
    34501 taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg aactgctagg
    34561 caggatcatc atcatcatgc cgccgcacaa aataccgact atcacctcgc aaacgcgcgc
    34621 ctgagcgata tcccacagct ggctggcctc agcaatatta accatcggaa aggcgatgat
    34681 ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca
    34741 ggcccaggta caaaagccaa gccacgccga catgctcaat agaaaaaacc acggctcatt
    34801 gagcgtatgc ccggcaagaa gcagtgccgc aatggctccc agtaaactgc cagcgatgcg
    34861 tccgaggctt ttgctgatga caccgccaac ggtgggaaag ctaaccactg cagccgaggt
    34921 catcgcccaa tagggttcat ccagattcaa ataataggca accgtcagcg ccagacacat
    34981 ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc gtggccttga accagggcaa
    35041 attgcgcaag gaccatgacg atgcgttcat tattgaccta cggcaatgct acaggtggtg
    35101 cccgacacca gcgtgacatc gcctggaact ttatccagcg caaagcggac gggaacgcgt
    35161 tgggcgagac gaacccaggg gacgttaggt ttcacatccg gaataaggct tgagtcactt
    35221 tcaacgcttt gatcataaat cgcccgaccg atactggaaa cgtgaccccg taacgttttg
    35281 ttatcactat agagggtaat ttgtgctggt gcaccttcgc ggatatggcg caacttggtt
    35341 tcttcaaaat aaccaatgac gtaaaacgag tggctgtcga caagggcaaa cagcggtttc
    35401 ccggtgtcag cgtaatcgcc gatgcgggta gtgaggttag tcacccaacc actcaccggg
    35461 gcgcgaattt ccgtttgcgc cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca
    35521 ttaacactgg cctgcatcgc tttaacattg aggttagcgg tatcgagttc ttcggcagaa
    35581 ataaaatttt gtgagagatg acgacgacga ttcgcttcgt tattggcttt tgcgaggtca
    35641 gattgcgctt tcgccagctg agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta
    35701 tcgatcgtga gcagaagatc ccccgcgtta accagttggt tgtcttttat attcagctca
    35761 acaatacgtc ccgacacctg tggggtgata gaaacctttt ctgcgcgtat tttcccgtcg
    35821 cgtgtccacg gagattgcat gtaataattc cacagccacc atccggcaag aaccgcaact
    35881 atcgctacaa tgattgttga gaaatactta attgttttaa ttgacatagt taccacgcaa
    35941 tcagtattgc cagagcaagg caaacgcaaa tcgcaaacag cgataaatcc attaacaagg
    36001 gatgccagat gtcaccggcg tagatccagc cacgaagcag gcggtgtacg acaagccaga
    36061 tgacgaatcc aaacgcgaag gctttgaaaa acggaggaaa atagacggac gcaccgaaca
    36121 ccagatcttg taagggcaat cctgttgcat tgagcataaa cttcacaaag tagattcctt
    36181 tacgaccgaa tatattgcgt tagattaata aatattcttt aagtgcgaaa aatttacgcg
    36241 caatttctga aaaatatgtc tgacggtaac caaatgcagc aatacatttg ttttagcaat
    36301 acaattgctg cacactattc taaaagccgc ataatatctt agcaagctaa ttataaggag
    36361 atgaaattgg aatcgccact aggttctgat ctggcacggt tggtgcgcat atggcgtgct
    36421 ctgatagacc atcgcctgaa accgctggag ttaacacaaa cccattgggt tacgttacac
    36481 aatatccatc agttacctcc agaccagtcg caaattcaac tggcaaaagc gattggcatc
    36541 gagcagccat cactggtccg tactctggac caactggaag aaaaagggtt aatttcgcgt
    36601 caaacttgtg ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca
    36661 ctgatcagcg aaatggaagc tgttattaac aaaacccgcg cggaaatatt acatggcatc
    36721 tccgcagagg aactggagca actgattacg ctcatcgcaa aacttgagca taatatcatt
    36781 gagttacagg ccaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg accacacgca
    36841 aacttattta gcgcggagaa acggtcacct gactgccatt gctggccagt acgacacgtt
    36901 ggcccggaga gaaacgagtg ttgccttgtt tctgtaccac catgatggta ttaccatcgt
    36961 ctttacgaat ttccagctcg acaccctgcg ttttgttcat tgcactctgt acgccctgac
    37021 cagctacgcc acctgcaaca gcgcctgctg cagtagccag agaacgcccg gttccgccac
    37081 caacagtatt ccccaggaaa ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg
    37141 aatcatcacc gccctgaatc tgtaccggac gtacgttaac gatggtgcca tagctgacat
    37201 tctgtacttg tttcgcttca gaagcggtat aaacatcccc tgacagggtg tcgttattaa
    37261 cacaaccgac aagagacaga cctaccattg aaacaaccaa tacgcgttta atcattgaaa
    37321 aatctcctgt tcaccatgaa acgccacgca agcatccctc atggtcaaag tatatggcat
    37381 atgtgaatcc atggttacat gttctaccaa atatgagcaa atcataatga aagtaggatc
    37441 aaccaattat aaacaaaaca ttcatgatga ttattgattt gaagataatg ttttactacc
    37501 aacggtaaaa atcggtcatt ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg
    37561 caaacgtcag attgaaaaag gatgaactat gaaatcgggc cgctttattg gcgttatgtc
    37621 aggcaccagc cttgatggtg ttgatgttgt gttggcgaca atagatgaac accgggtcgc
    37681 acagctggca agcttgagct ggccgatccc ggtgtctctg aaacaggctg tactggatat
    37741 ttgccagggc cagcagctta cactttcgca gtttggacag cttgatactc aactcgggcg
    37801 actttttgct gatgcggtca atgccttgct taaggaacaa aacctgcagg cgagagatat
    37861 agttgcgatc ggctgtcacg gtcaaaccgt ctggcatgaa ccgacgggcg tggaaccaca
    37921 cactttacag attggcgata acaatcaaat tgtggcacgc accggaatta cggttgtcgg
    37981 tgattttcgc cgtcgcgata ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt
    38041 ccatcatgcc ttgctggctc acccaaccga gcgacgaatg gtgctcaata ttggcggcat
    38101 cgccaatctg tcactgctca ttcctgggca gccggttggc ggctacgata ccggtcctgg
    38161 taacatgctg atggatgcct ggatctggcg tcaggccggt aaaccttacg ataaagatgc
    38221 cgagtgggca cgggcgggta aagttattct cccactgctg caaaatatgc tcagcgaccc
    38281 atatttctcg caacctgcac cgaaaagcac cggacgcgaa tactttaact acggctggct
    38341 ggagcgccat ttacgccatt ttccgggtgt tgatccccgt gatgttcagg ccacactggc
    38401 agaactcacc gccgtgacca tttctgaaca agttttgttg agcggtggct gcgaacgatt
    38461 gatggtatgt ggtggaggta gtcgtaatcc gctactcatg gcgcgtctgg cggcattact
    38521 gccaggcaca gaagtcacca ccaccgatgc cgttggcatt agtggcgatg acatggaagc
    38581 attggctttc gcctggcttg cctggcggac gctggcggga ttaccaggaa atctgccttc
    38641 cgtcactggc gcaagccagg agacggtact gggggctatt ttccctgcta acccgtgata
    38701 tcagagttaa ctgaattttt cttaccgcgt ctgccgttac acttggaacg ttagggaggg
    38761 cgtattgccc tccagaccag gaaagtcttc gggaaatgtc tatgaaaaaa ctgttactga
    38821 tctgtttgcc ggtgcttctc accggctgta gcgcgttcaa tcaactggtt gagcacatgc
    38881 aaaccgctac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc
    38941 cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa cagggcattt
    39001 cagcgtctgg tgcacgttac actgacggaa tctatgtttt ctggtcgaaa ggcgatgaag
    39061 cgactgtcta taaacgcgac cgcatcgtat tgaataactg tcagttacaa aatccacagc
    39121 gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa
    39181 accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa
    39241 ggcgggttac gccgtcgcga tcttcccgcc gatccattaa ccctttttga acgttggctc
    39301 tctcaggcgt gtgaagccaa actggcagac cctaccgcga tggtggtcgc taccgtggat
    39361 gaacatgctc agccttatca acgcatcgtt ttactcaaac attacgacga aaaaggcatg
    39421 gtgttttaca ccaacctcgg tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt
    39481 agcctgctgt tcccgtggca tacccttgaa cgccaggtga tggtgatcgg taaagcagaa
    39541 cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga tagccagatt
    39601 ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa
    39661 ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag cttttggggc
    39721 ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca tcgcctgcat
    39781 gaccgctttt tgtaccagcg agaaaatgat gcgtggaaga ttgatcgtct tgcaccctga
    39841 aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact ttattctatg
    39901 tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg
    39961 agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg ctggtagccc
    40021 aggtgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc gcgctctatt
    40081 gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca ttgttatgcc
    40141 tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc
    40201 tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa gaaactgttc
    40261 aggagtgggt ggacaaaatc cgtaagcagg ttgccccgtt cctcgatttc gactgtggag
    40321 aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat gtgctgacct
    40381 tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa gaagcggtta
    40441 agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc tacaacctgt
    40501 tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg caaattggtg
    40561 gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt ctgcatcaga
    40621 atcaggtgtt tggcctgacc gttccgctga tcactaaagc agatggcacc aaatttggta
    40681 aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac aaattctacc
    40741 agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc ttcaccttta
    40801 tgagcattga agagatcaac gccctggaag aagaagataa aaacagcggt aaagcaccgc
    40861 gcgcccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac
    40921 aggcggcaaa acgtattacc gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag
    40981 cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa aagggcgcag
    41041 acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa
    41101 ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct gaatacttct
    41161 ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt
    41221 actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga aatcactccc
    41281 cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc tcacgttgtt
    41341 tatggtcatg cgggtaacag tgcggcagag tttcctatgc gccgcctggg cgcgaacgtc
    41401 tggccgctga acaccgttca attttctaat cacacccaat acggcaaatg gactggctgc
    41461 gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgccat tgataaatta
    41521 cacacctgtg atgccgtgtt aagtggctat ctgggatcgg cggagcaggg tgaacatatc
    41581 ctcggtatcg tccgtcaggt gaaagccgcg aatccgcagg cgaaatattt ttgcgatccg
    41641 gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg
    41701 cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc
    41761 tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga actcattgcg
    41821 caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt
    41881 tttgaaatgc tgctggtcac cgccgatgaa gcctggcata tcagccgtcc gctggtggat
    41941 tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact gctggtgaaa
    42001 ctgcttcagg gggcaacgct gcaggaggcg ctggaacatg tgaccgctgc agtctacgaa
    42061 atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc tgctcaggat
    42121 cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg
    42181 cccacaacga cctgtgggcc gatggatatt actttaagcc ttccgctgac agcgcgtctt
    42241 gtacttccgg acgttcagcc atacgttgca taaatgctgc aatgtgctct aacccttcca
    42301 gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg
    42361 taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct
    42421 tcttctccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatca gggcgaaaca
    42481 gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat
    42541 agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat
    42601 actgcataat cgctacgcct tccgtcagca aagtaccgtc atccagcagc aatgcaggca
    42661 cctgcccctt agggttaacg gcaaagtaat cgtcaccgtt ttcgagacgt tttttcatta
    42721 aatccacact gacgagggta aaatcctttc cgctctcacg cagggtgata tgggaagcga
    42781 gagagcaggc acccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta
    42841 aagtatggta gtgcgttgct gaacaaaaaa aagccgctaa cgaaaagtta gcggcttgta
    42901 aaagagtttc cctgaatttt acgctacagc agctttcgcc gctttgtctg cagcgtcatc
    42961 ctgcgtcata cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taatggcagt
    43021 agcgacacca atctgcaaga atacgcgacc ataaacttcc agtgacatca gcgggtcggt
    43081 aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
    43141 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
    43201 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
    43261 tgcgaccagc cagcttacag acacgatacc agcgtcagac gcgaatttcg cacccagcgg
    43321 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
    43381 cagggtatcg cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
    43441 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
    43501 aacgttacga atcgcaaaga agttcagtga tgtcggcatc tggctgtaca gcacgaagaa
    43561 gatgattgct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
    43621 catcgcgaag gcttctttac cgaagataac cacgataccg aaggcaacaa cgcccagcgc
    43681 catacgcgca acttcctgat tgtgcagcag ccaggtggcg atagcgatca gtgccacaac
    43741 accaataatg gttagcagca ggttacggta gttgataggc tcgaagtctg gttttgaacc
    43801 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
    43861 aacgctcaat gcaaacgcaa cgctccagcc gtatttcgcg gccagccacg gcgtagcaat
    43921 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
    43981 acgcgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ccttaaacag
    44041 gccgttaccg accgcaatag ccgccatacc catataaaca ataccggcgt cgtgaccaga
    44101 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
    44161 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
    44221 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
    44281 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
    44341 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
    44401 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
    44461 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
    44521 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
    44581 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
    44641 cgtaacggca gattttttca ctgcactgag aggggggtga tggcttaacg gcgatatgtt
    44701 ctgctattta gcgtttttac tagtgagata gtccagtttc tgaaaaatag ccagtgtaat
    44761 gttttgtagg tcaatatttg ttgcgtgtgt aaatttaaat gattttaacc tgatttttgc
    44821 aatgttttta cattgccaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
    44881 attaaaaaaa taattaacct gcatttttgg ttatccgcaa acggattacc tgatgataac
    44941 gctaagcaat ggcatcattc acgttaaaaa gatgttttaa aagaggataa agaaaggtta
    45001 tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactttctct ttgtattcac
    45061 aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc
    45121 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc acttttagta
    45181 gcttttcttc tacctgttcg acatttttcc ccggcgcaaa ttgagtacga ttacaaacgc
    45241 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt
    45301 tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacca
    45361 agccattatg ctgctccagt aagatacggc aggttttgat gatattttct gctttgctgt
    45421 tataaagccc aatcgttttg atataggttt taaccccttc aacgcccagt tcaagcatcg
    45481 ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca ctgacatcgg
    45541 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
    45601 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccagg cgttttgctt
    45661 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagt
    45721 acggcgcttt ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaggcc
    45781 aataaatgca cctggaggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatttc
    45841 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaatgtgcc
    45901 attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccatacc
    45961 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
    46021 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
    46081 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
    46141 catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt
    46201 caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cgttagtggc
    46261 agtggacgtg accgccaaca gaggacaaag gccgagcaac tggaccagcg cagagttgtt
    46321 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc
    46381 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
    46441 attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc
    46501 tttcttcacc gcccagtgcg catcatctgc accactgatt tttttacccg caaaatgggt
    46561 gatccagtca gaaaggcgca gttcgatttt atcgccaagc cctggcgttt cgtggtgctc
    46621 tgtcacgcgc gtgccaagta ccgtgccgtt aaaatcggct cccaccagca gctgaatcgc
    46681 accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg
    46741 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
    46801 actctgtgcc agcgcattgt tatagcgttc ggctggcagc acctgatcaa ataatgcctt
    46861 ttgttgcaga ctggcctgtt cagcaatcgt cgttttggtc atctggttga tggccgcagt
    46921 taaccctgtt gaacccgctg caaatagcgc taacgtaatg ccgtgttttc ggatagtttt
    46981 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
    47041 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
    47101 actgcggatc aaccagacta ataagcccgc cagcgcgccg aaaataagac gaccacgatt
    47161 ggtcgtagaa gcggtaaccg ggtcagtcaa aataaagaat gcgccgagca tggtcgcacc
    47221 agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt
    47281 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
    47341 ccatagcaac cacaggccgc cagccagcca ggcgagattt acccattgcc aaccagcgcc
    47401 cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat gaccggcacg
    47461 gacagaggtt ttaaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg
    47521 tagtgtgttc atatcaccac cactggcggt atgtccgctg aaaataacct gtatggcgtc
    47581 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
    47641 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
    47701 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca gcacgaccat
    47761 ccaccatggc gcgagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
    47821 gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc
    47881 taacagagca ctaaccgatg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
    47941 cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt
    48001 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
    48061 tacaaccttc tgctgggcgg cttttttggc ctgagcgcgg gcaatagccg cggcaactgc
    48121 cgctttgcgc ggatcaacct gttcttctgg ttctgcgtta gtctgttgct gttccagctt
    48181 gcgcgctttg gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgacctgttc
    48241 ttctggttcc gcattagcct gttgctgttc cagcttgcgc gctttggcgc gggcgatagc
    48301 tgcttcaacg gcagttttac gtggatcagc aacggttgct gcgtcgttag tttgctgcag
    48361 ttctgcctgt ttcgctctgg cttgcgcttt acgggcttcc cgtgctgcaa taattgcact
    48421 gttatccggg cgttcgcccg ctttaatcac aataggctgt gtagcctggg cctgtttctc
    48481 tttcacccgc gccagagcgg cagcaatcgc atctttatct ttggctgcag gttgaacggc
    48541 tgcgctctta tgtcgttcaa ggcgagccgc tttttcgcgc tccagacgag cctggcgcgc
    48601 ttcgaaacgc gctttggctt ctgcggcgcg cttttcttcc tgacgaatag ccgcaatttc
    48661 agctttttcc tgacggaaat attgcaccag gggaatattg ctcgggcaaa cccacgcgca
    48721 agccccacat tcaatgcaat cagcaatgtt atgcgtggta gctttatcgt gttgctgacc
    48781 tttgctgaac cagtacaact gttgcggcaa aagatccgca gggcaggcgt cagcacaggc
    48841 gctacaccgg atgcagcttt gttcttcctg tggttcgcca agttcattgg cagagggagc
    48901 caacagacag ttggtaatct ttacgaccgg gacatccagc catggcaagg taaagcccat
    48961 tagcgggcca cccataatca ccatttgatc ggcagagggg cagaatccgg catcattcaa
    49021 taaatgacgc actggcgtcc ccagccgtgc ccagacgttg cccgggcgag cgattgcttc
    49081 gccagtcagg gttacaacac gctcggtaat cggctcgcca tcaataacgg cacgtttcac
    49141 tgcataagca gtgccgacgt tttgcattaa tacgccgata tcggatgaac gcccgccatg
    49201 tggaacctgc ttcccggtca gaatgtaggt taattgttta gcaccgccag aaggatattt
    49261 ggttggaatc acccgcagag aaatatcgtt agagtccgcc agcaccgcgc gcagcatgga
    49321 aatcgcctgc ggtttgttat cttcaatgcc gataagaatt tcgcgtggct gcagaatatg
    49381 cgcaagaatg cgaataccct ctacgacctg agccgcgcaa tcctgcatca aacggtcatc
    49441 ggcggtaatg tacggctcgc actcagccgc gttgataatc aacgtttcaa tcttatctcc
    49501 gccaccctgc aatttaacgc ctgtcgggaa tcctgcaccg cccagcccgg caacaccaaa
    49561 ctgatgtatg cgctcgatta actcttcgcg actgcgagtg cgataatcgg cccagccgtc
    49621 gcgcgggatc cagcagtctt caccatcggc atcaataatc acgcttaatt cagctaaagc
    49681 tgaaggatga gccgtagagt ggggcgcaat agccgtaacg gtacccgagg tgggcgcgtg
    49741 aacaggcagc attttgccgc gaccacgggt aagcggctgg ccgcgcaata ctttatcgcc
    49801 gacgctaacg cacaactcac cttcagcgcc aatatgctgt ttcagtggaa taacaaaacg
    49861 ctgcgccagt ggtacctggc gcaggggtgt accgttggac tgggttttca tctccggtgg
    49921 atggatgccg ccgttgaaat cccagatttt attttttctg aatgcagaga ataacttaag
    49981 catggtgttc cacgggaatg atacgcacgg gaatggtgtt cagatcccat ttccaggagt
    50041 caggtgtttc tgcgaccggt tgcaacgaga tgcagtgcgt cgggcacgga tcaacacata
    50101 aattgcagcc cgtacagaga tcactcatta ccgtatgcat ggcacgggta gcgccaacga
    50161 tggcgtctac cggacacgcc tgaatacatt tagtgcagcc aatacagtta ttttcatcaa
    50221 taaccgccac catccgcgca ggcgttatct cttgcgcttc gccatccagc ggctgcggct
    50281 cgacattaag caactcggca atttttagca tcacagcttc gccacctggg gcgcaacggt
    50341 tgattttttc accgttacag ctgatggctt ccgcgtaggg gcgacagccg ggataaccgc
    50401 actgaccaca ctggctctgc ggtaagattt cgtcaatttt ctcaacgacc ggatcgtctt
    50461 ccaccgcaaa acggcgggag gcataaccca gaatggcgcc aaacgccagg cccagcaggc
    50521 tcacggcggc aacggcaatc cagatagcat tcattacaac ttcaccaaac cactaaagcc
    50581 cataaaggcc agagacataa gacctgcggt aattaacgca atggcatgac cgcgaaaagg
    50641 tgccgggaca tcagccacag caaggcgttc gcggatggcg gcgaagagca ccatcaccag
    50701 cgagaaaccg atagcggcgg aaaaaccgta cagcgccgac tgcaagaaat tgtgcccgag
    50761 attgatattc agcaacgcca cgccgagcac tgcacagttg gtggtgataa gcggcaaaaa
    50821 aatccccagc agacggtaaa gcaccgggct ggttttgcgc accaccatct cggtgaactg
    50881 cacgaccaca gcaatcacca gaataaatgc cagggtgcgc aggtaaatca gattaagtgg
    50941 gatcaaaatc cacgtatcga taagccaggc gcaaatagac gccagcgtca tcacaaacgt
    51001 tgttgccagc cccatgccca tcgcggtttc cagttttttg gaaaccccca taaacggaca
    51061 gagaccgaga aacttgacca gtacaaagtt attgaccagt acagttccga caaacagtaa
    51121 caggtagtca gtcatttttc ggcctgaaat aaaaaagccg cctattatcc gttaatccag
    51181 agcaggcgac aacaggttaa ctgtaaggtt attacggatt cacaaacgtc gcttttaccc
    51241 gcgacgagcg tttgaaatag gggacgatca atgcggttgc caacaaagta aacagcaact
    51301 ggcgaacggc tatgccatct tccacgggtg aaaaagcgaa tgcttttact gccagcaata
    51361 cggaaataag cagccagata atatagtgtt taggaacaca acgccgacgt ttaaagaaag
    51421 cgatggtcaa ccacagtgtg taataccaca tggcgatagc ggtaataaaa gaaacgcccc
    51481 ataagatttg tgtcgtcagt gcttgtccgc cgagcgtttg aaatgtttgc ggagaagata
    51541 acgcagcggt atacagcaac aacgccagcg tcgtactcaa cagagcgact aaaagccagg
    51601 ccaacgggcc aagcaaccag cctccaatac gttgtggcgt tgtggtgatc ataggttctc
    51661 cagaatagcg acgcaaacag gcaaatctaa ttttaggggc gttattataa ggcttttata
    51721 accgatcgct actttcttta ttggacatag tgccagacgg acttcggtac ctggccgagg
    51781 tcaaacaaac gcccgccaga aaccagctct gcgcgacggt gatcggcagc acgatacata
    51841 ttgatcagtt cctgatcgtc cgtcagggtg taattaagat ggtcgtagag tttttccaga
    51901 cttttgagtg aactgatttt gcgaaatttt aataagtagt cctgaacagt cataaaaggt
    51961 ccataaaata attaatacca aaggggtagg tattattagt gatgataaaa tttcaccaaa
    52021 agtgaacgct tgtgttatta caataataag aatgcgctga tggcaaatat tcagggatga
    52081 aaaactgctc tgaaaagagt aacgcgcttt actatccctg acaatcacca acaacatcga
    52141 acaagataat aaattcctgg tttaatatcc gacaagtgaa aacatgcacc cggacgggca
    52201 gcatgtcgct ccacaagtgc agagcttact tgtgttgtac cgaagcactc tgttcaggtg
    52261 gctgatagtt gtcaatgtga ctcgccacgc caagaagaat gactgaaacg acaagaacga
    52321 tccaacctgt taattcaata agacgattca ttacagccca catctctctt gattgatcca
    52381 ttaacttcag ggggtaaatg ttacttagca ataatagctc cagcaagatt tttactgagg
    52441 tttttgcgat atttagcttt tgtcgttgga aaattcgcta tttttttgac ttaagttaaa
    52501 caacatccct tattgctggc aggttattaa actgttgagc gtgggtaaag gatagtgtca
    52561 aatagccatc atacttcaat gagaggcaat gacatgagcg acaacatccg tgttgggttg
    52621 attgggtatg gttatgcgag caaaaccttc catgcgcccc tgattgcggg cacgcccggg
    52681 ctggaactgg cggtaatctc cagcagtgat gaaacaaaag taaaagccga ctggccaacg
    52741 gttacggttg tctctgagcc gaagcatctg tttaacgatc ccaacataga cctgattgtc
    52801 attcctacac ccaacgatac ccatttcccg ttagccaaag cggcgcttga ggcgggtaaa
    52861 catgtggtcg ttgataaacc ctttaccgtg acactgtcac aagcgcgaga gctggatgcg
    52921 ctggcaaaaa gcctggggcg tgtgctgtct gtattccata accgtcgctg ggatagcgat
    52981 ttcttgacgc taaaaggttt actcgcggaa ggcgtgctgg gtgaagttgc ttactttgag
    53041 tctcattttg accgcttccg tccgcaggtg cgcgatcgtt ggcgtgaaca gggcggccca
    53101 ggcagcggta tctggtacga tttagcacca catcttcttg atcaggccat tacgcttttt
    53161 ggtttaccgg tcagcatgac ggtagatttg gcacagttac ggcccggagc gcagtcgacc
    53221 gattatttcc acgccatctt gtcctatcca cagcggcgag tcattttaca cggtaccatg
    53281 ctggcagccg ctgagtcagc acggtatatc gtgcatggat cccgaggcag ttatgtgaaa
    53341 tatggcctcg atccacagga agaacgtctg aaaaatggcg agcgtctgcc gcaggaagac
    53401 tggggctacg atatgcgtga tggcgtactt acccgcgtgg aaggtgagga acgtgtcgaa
    53461 gaaacgctgt tgacggtacc tgggaattat ccggcttact atgcggctat tcgtgatgcg
    53521 ttaaatggcg atggtgaaaa tccggttccg gcaagccagg caatccaggt aatggagttg
    53581 attgagctgg gcatcgaatc cgccaaacat cgcgcgactt tgtgccttgc atgaaaaatg
    53641 gatggtgcaa tttgcaccat ccattttgtt acttcgcggc gactttttct cgcagtgcgc
    53701 gtttttcctc agcgctgagg aaagccattt ccagaccatt aatctgtgcc tggcggattt
    53761 gctcgcggga taacccagca gctggcgcgg caacggtata ttcgtgaatg atatccactc
    53821 cctgtacgcc gggatcgtca gtgttaatgc tggcacgaat gccatgctca aggaacgttt
    53881 tcagcggatg tgcagccagc tctgctacgg tgctggtctg aatattggag gtcagacagg
    53941 attcaatacc aatttgttgc tcggcgagaa aatccatcag cgcccgatct tcaatggctt
    54001 ttacgccatg tccaatacgc tccgcaccca gttcacgaat cgcctgccag atgctttccg
    54061 gcccggcagc ttcgcctgca tggacggtaa tatgccagcc cgcatcacgc gcgcggttga
    54121 agtgagaaag gaacagactt cccgggaaac caagttcatc accggctaaa tcaagtgcgg
    54181 taatctggtc acggtgggct aaaaaggcct ccagctcttg ctgacaggcg gcttcgccga
    54241 aggtccggct cataatgccg ataagcttcg cctgcacacc aaaggtgcgg caaccttcac
    54301 gtacgccatc gatcaccgct tcgacaacac ccgctacagg cagctgatgt gccattgcca
    54361 tgtagcctgg tgaaaaacgc agctcgacat agtgcaggcc gtgacgggct gcatcttcaa
    54421 tgttttcaaa tgccacgcgg cgacaggcat caagagaggc gagaacttta acgccccagt
    54481 caagtttggt cagaaagctc accagatcgg gttcgttggc aatgacctga acgtggggaa
    54541 tcagtgtttc cagggattgt gcaggaagcg agatattata ctggcggcca agttcaagaa
    54601 tggtctgggg acgaatgttg ccatcaaggt ggcgatggat atcagttaat ggcagggtgg
    54661 tatcaatcat ggtcgcactc tttttctgaa tataaaagtg cgcaccatta taaaaacaaa
    54721 gcagggtaaa aatccacgat tgcgcaacga ttttttacgt tgcgcaattg gggttagcga
    54781 acagcgcgga tggcgtttat taatccagcc acaccttttt ccagtttcga acgtgggcag
    54841 ccggcattga gacggacaaa accacgacct tcttcaccat aggtataccc cggcatgatc
    54901 gcgacttttt cttgctcgat aagcgctttt tgcaacgcgt tgtcgtcaat attcaacaga
    54961 cgtagatcaa gccaggccag ataagtggat tgcgggatct gccagttgag ttcaggaaac
    55021 gcggcgttca ttttatctga gatatacgtc aggttatctt tcagatagac gcgtaaggca
    55081 tccagccacg gtgcaccttg ctgataggcg gcgatatggg cagttaacgc cagtaccgaa
    55141 ggggaagaaa gcccatcacg tcctttcagt gccgataaat aggcatcacg gctgctgcta
    55201 ttttctataa tcccgtaagc accggtcagc gcgggaatat tgaaactttt cgagcccgac
    55261 gtcagcaacg cccagtctcc ccgcgctaca ttactccagg gaatatgcgg ctgctcgccc
    55321 cagaccatat ccatatggat ttcatcggaa ataacccgca caccatgacg ctcgcatagg
    55381 tcagccatga tctccagctc atcgcacgtc cacactttcc cggtaggatt ctgtgggcta
    55441 cacaggagca taattttaca ttctggtttc gccaacacgg cttccaactt gcccatatcg
    55501 caaaaccaac catcagcctg cttctctaaa gcaacgggca ttactgtgcg ctggttacct
    55561 tcaatggcct tgtaaaatgc gtcataggcg ggtgtgtgga tcaccacgcc ttcacctgtt
    55621 tcagaccact gacgaatcag ttctgaaacc atatagatga cagaagggcc atacaccacc
    55681 gtctgagaat cgatggcggt gtaatgctgg gtggaaaacc agtgggcaat agccgcgaga
    55741 aactcatcgt ttttccagcg gctgtagcca aatacgccgt gcatcaggcg ctgattcagc
    55801 gcctcgataa tgcagggggc agtggcaaaa tccatgtctg aaatcgtgaa cggtaacagg
    55861 tcagcagtgc cgaaacggtc agcgacataa tcccactgtg tacaccatgt gccatgacga
    55921 tccacgacct ttgaaaaatc gaacatatct tatccttatg cctggacagt atgcatcaga
    55981 ccggccattt catcttttac tgactgaact tgtggcccga taacaacctg caggttatgt
    56041 tgattaagtt gtactacgcc aattgcccga ttgtccttca gtgccagcac attaacaagc
    56101 gacatatctt tcacagacaa acgcagacgg gtaatgcagt tatcgaggct gacaatattg
    56161 tcggcaccgc ctaatgcttc gaggattgca ggaacgttgt aacctgattt acccggcgca
    56221 ccggcaacgg ctttttcgat tgagctggca acttcgctat cgcgccccgg ggttttcaga
    56281 ttgaagcggg tgatagcgaa acggaagatg acgtagtaaa cgacaaacca gattgccgcc
    56341 accactggca ccatgtacca cttggttgac agaccatgca aaataccgaa caccacgaag
    56401 tcgatgatat tgccgtcggt attaccgatg gtgacgccga gcacagacat gacggtgaag
    56461 ccgaggccgg ttaacagcgc gtggatgaca tacagaactg gcgctacgaa caggaacagg
    56521 aattccagcg gttcggtagt gccgccaacg acgcaggcga tcaggccaga aatcagcaga
    56581 cctttaattt tatggcgatt ttccgggcgc gcgcagtgat acatagctaa cgctgcacct
    56641 ggcagaccgc cgagaaacgc aggcatttta ccttgcgaaa ggaaacgcgt ggcgctttca
    56701 gaaaaaccgt gagtggtcgg gcaactcaat tgcgcctgga agatggtcaa tgcgccgctg
    56761 acggtttgac cgcagacttc ctgcgtgccg cctgcgtcgg taaagcgaat taatgccacc
    56821 agaatgtgat gcagaccaaa cggcaacagc agacgttcac cggtaccaaa cagcatcggt
    56881 ccgaaatcac ccgcgctgtt tatcatatgg cccaagccgc taatacccat cgcgaaaatc
    56941 ggccagacta atggaatcac caggccgaca aggcccatca ccagcgagga gatgattggt
    57001 acgaagcgcg taccgccgaa gaatgcaagc gcatccggca ggcggatatt atggaatcgc
    57061 tcatgcagca tccagacgat aataccggcg atcaccgcac cgaggatacc ggtgtcgatc
    57121 gactggatcc caagaatgct ctggatgtta ttggctttca gaaccgcggc gtccgtggtt
    57181 ggcagaatgc ctttattggt caaccagaag tttaccgcga ggttcattac cgcataaccg
    57241 acgaagccag cgaatgccgc tacgcctttg ttttcgcgtg ccagacccag cgggatggcg
    57301 atacagaaca tgactggcag gaaactaaaa gcaaacgagc caatcttact catccaggta
    57361 aagatagcct gcaacacggg gttgcccagg accgggatca gggtgatgac atcgtggcta
    57421 ctaagagaac taccaatgcc gagcataatg ccgcagaacg acaataatgc cacgggtaac
    57481 atgaaggttt tgcctaactg ctggaagaac tcccacagcg tgactttcgg tgctgttttc
    57541 gccgtcataa aacgactcct cgtagagaac aatctggatt cagtaaattg tgcgagaaga
    57601 taaaacgttt tatcaaattt tagtgaggca taaatcacat tacgcaacga taatagcggg
    57661 tataagataa ataaaaggta aaacgtttta tctgtcacat aatcagggag taggtcatct
    57721 gcatggctac cgccaaaaaa ataaccattc atgatgttgc gctggctgcg ggcgtgtcgg
    57781 taagtaccgt ttcgctggtg cttagtggca aagggcgaat ctctaccgcc acaggagaac
    57841 gcgttaacgc cgccattgaa gagctgggat ttgtgcgcaa tcgccaggcg tcggcgctgc
    57901 gcggcgggca aagcggcgtc attggtttga tcgtccgtga tttatctgcg ccgttttacg
    57961 ccgaattgac ggccggattg acggaagctc tggaagcgca gggacggatg gtttttttgc
    58021 ttcacggcgg taaagacggc gagcagctgg cacagcggtt ttcactgtta ctgaatcagg
    58081 gtgtggatgg tgtggtaatt gccggggctg caggaagcag cgatgacctg cgacggatgg
    58141 cagaagaaaa agctatcccg gtgattttcg cttcccgtgc cagttatctt gatgatgttg
    58201 atacggttcg cccggacaac atgcaggctg cacagttgtt gacggagcat ctcattcgca
    58261 atgggcatca gcggatcgcc tggctgggag ggcaaagttc ctcattaacc cgtgcagaac
    58321 gggtgggggg ctattgtgca actctactaa aatttggcct gccgtttcat agcgattggg
    58381 tgttggagtg cacttccagc cagaagcaag ccgcggaagc tatcacggcg cttttacgtc
    58441 ataacccgac catcagtgcc gtggtttgct ataacgaaac tattgcgatg ggggcatggt
    58501 ttggtttgct caaagcaggg cggcaaagcg gggaaagcgg agtcgatcgt tactttgagc
    58561 aacaggtttc gctggcggca tttaccgatg cgacaccaac cacacttgat gatatacctg
    58621 ttacctgggc cagcacgcca gcgcgggaac ttggtatcac acttgcggat cgcatgatgc
    58681 aaaaaatcac ccatgaagag acgcattcac gcaatcttat tattcccgcc cggctcattg
    58741 cggcgaaata atcctctctt tatctgctat acctggtagt gtcctttcct caaggttaat
    58801 gataaaaaat gtggcacaca ggcggtgcct tttattttta ctgaagttca caggaggttt
    58861 atgtgtttaa ttctgacaac ctgagactcg acggaaaatg cgccatcatc acaggtgcgg
    58921 gtgcaggtat tggtaaagaa attgccatta cattcgcgac agctggcgca tctgtggtgg
    58981 tcagtgatat taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactgggtg
    59041 gtcaggcatt tgcctgccgt tgtgatatta cttccgaaca ggaactctct gcactggcag
    59101 actttgctat cagtaagctg ggtaaagttg atattctggt taacaacgcc ggtggcggtg
    59161 gacctaaacc gtttgatatg ccaatggcgg attttcgccg tgcttatgaa ctgaatgtgt
    59221 tttctttttt ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggcggtggcg
    59281 ttattctgac catcacttct atggcggcag aaaataaaaa tataaacatg acttcctatg
    59341 catcatctaa agctgcagcc agtcatctgg tcagaaatat ggcgtttgac ctgggtgaaa
    59401 aaaatattcg ggtaaatggc attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg
    59461 ttattacacc agaaattgaa caaaaaatgt tacagcacac gccgatcaga cgtctgggcc
    59521 aaccgcaaga tattgctaac gcagcgctgt tcctttgctc gcctgctgcg agctgggtaa
    59581 gcggacaaat tctcaccgtc tccggtggtg gggtacagga gctcaattaa tacactaacg
    59641 gaccggtaaa caaccgtgcg tgttgtttac cgggataaac tcatcaacgt ctctgctaaa
    59701 taactggcag ccaaatcacg gctattggtt aaccaatttc agagtgaaaa gtatacgaat
    59761 agagtgtgcc ttcgcactat tcaacagcaa tgataggcgc tcacctgaca acgcggtaaa
    59821 ctagttattc acgctaacta taatggtttt atgatggata acatgcagac tgaagcacaa
    59881 ccgacacgga cccggatcct caatgctgcc agagagattt tttcagaaaa tggatttcac
    59941 agtgcctcga tgaaagccat ctgtaaatcc tgcgccatta gtcccgggac gctctatcac
    60001 catttcatct ccaaagaagc cttgattcag gcgattatct tacaggacca ggagagggcg
    60061 ctggcccgtt tccgggaacc gattgaaggg attcattttg ttgactatat ggtcgagtct
    60121 attgtctctc tcacccatga agcctttggg caacgggcgc tggtggtcga aattatggcg
    60181 gaagggatgc gtaacccaca ggtcgccgcc atgcttaaaa ataagcatat gacgatcact
    60241 gaatttgttg ccgagcggat gcgtgatgcc cagcaaaaag gcgagataag cccagacatc
    60301 aacacgtcaa tgacttcacg tttactgctg gatctgacct acggtgtact ggccgatatc
    60361 gaagcggaag atctggcgcg tgaagcgtcg tttgctcagg gattacgcgc gatgattggc
    60421 ggtatcttaa ccgcatcctg atcctctcat ttcgcgggct ggtgataact gtgcccgcgt
    60481 ttcatacttt aatttccctg tacaaaatac aatctagccg ggatcggaga attccccccc
    60541 aaaaatattc accgtagcca tatgtcatga gagtttatcg ttcccaatat gctcgaacga
    60601 acgttcggtt gcttatttta tggcttctgt caacgccgtt ttaaagatta atgcgatcaa
    60661 tatcacgctg tgggtattgc agtttttggt tttttgatcg cggtgtcagt tctttttatt
    60721 tccatttctc ttccatgggt ttctcacaga taactgtgtg caacacagaa ttggttaact
    60781 aatcagatta aaggttgacc agtattttta tcttaatgag gagtccctta tgttacgtcc
    60841 tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga
    60901 tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc
    60961 aattgctgtg ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc
    61021 gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat
    61081 cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt
    61141 gatggagcat cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc
    61201 cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc
    61261 gccgggaatg gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt
    61321 ctttaactat gccgggatcc atcgcagcgt aatgctctac accacgccga acacctgggt
    61381 ggacgatatc accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg
    61441 gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggagc aacaggtggt
    61501 tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc
    61561 gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat
    61621 ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa
    61681 ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa
    61741 aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa
    61801 ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca
    61861 tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg
    61921 tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac
    61981 tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag
    62041 cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata
    62101 tttcgcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt
    62161 caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg
    62221 cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt
    62281 actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga
    62341 atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga
    62401 gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt
    62461 cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg
    62521 cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct
    62581 gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg
    62641 aatcaacaac tctcctggcg caccatcgtc ggctacagcc tcggtgacgt cgccaataac
    62701 ttcgccttcg caatgggggc gctcttccta ttgagttact acaccgacgt cgctggcgtc
    62761 ggtgccgctg cggcgggcac catgctgtta ctggtgcggg tattcgatgc cttcgccgac
    62821 gtctttgccg gacgagtggt ggacagtgtg aatacccgct ggggaaaatt ccgcccgttt
    62881 ttactcttcg gtactgcgcc gttaatgatc ttcagcgtgc tggtattctg ggtgccgacc
    62941 gactggagcc atggtagcaa agtggtgtat gcatatttga cctacatggg cctcgggctt
    63001 tgctacagcc tggttaatat tccttatggt tcacttgcta ccgcgatgac ccaacaacca
    63061 caatcccgcg cccgtctggg cgcgggattg ccgcttcatt aacctttgtc tgcctggcat
    63121 ttctgatagg accgagcatt aagaactcca gcccggaaga gatggtgtcg gtataccatt
    63181 tctggacgat tgtgctggcg attgccggaa tggtgcttta cttcatctgc ttcaaatcga
    63241 cgcgtgagaa tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt ctgcaaaccc
    63301 tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg ctgatttcga
    63361 ccttcgcggt cagcgcctcg tcgttgttct acgtgcgcta tgtgttaaat gataccgggc
    63421 tgttcactgt gctggtactg gtgcaaaacc tggttggtac tgtggcatcg gcaccgctgg
    63481 tgccggggat ggtcgcgagg atcggtaaaa agaatacctt cctgattggc gctttgctgg
    63541 gaacctgcgg ttatctgcta ttcttctggg tttccgtctg gtcactgccg gtggcgttgg
    63601 ttgcgttggc catcgcttca attggtcagg gcgttactat gactgtgatg tgggcactgg
    63661 aagctgatac cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa gggctcacct
    63721 attcactatt cttatttacc cgtaaatgcg gtcaggcaat cggaggttca attcctgcct
    63781 ttattttggg attaagcgga tatatcgcca atcaggtgca aacgccggaa gttattatgg
    63841 gcatccgcac atcaattgcc ttagtacctt gcggatttat gctactggca ttcgttatta
    63901 tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgtggttgaa attgataatc
    63961 gtaaaaaagt gcagcagcaa ttaatcagcg atatcactaa ttaatattca ataaaaataa
    64021 tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta tttgcctgac
    64081 ggctgcctct ggccttacct ctgcttatgc ggcgcaactg gctgacgatg aagcgggact
    64141 acgcatcaga ctgaaaaacg aattgcgcag ggcggataag cccagtgctg gcgcgggaag
    64201 agatatttac gcatgggtac agggaggatt gctcgatttc aatagtggtt attattccaa
    64261 tattattggc gttgaaggcg gggcatatta tgtttataaa ttaggtgctc gtgctgatat
    64321 gagtacctgg tggtatcttg atggtgataa aagttttggc tttgccctgg gggcagtaaa
    64381 aataaaaccc agtgaaaata gcctgcttaa attaggtcgc ttcgggacgg attatagtta
    64441 tggtagctta ccttatcgta ttccgttaat ggctggcagt tcgcaacgta cattaccgac
    64501 agtttctgaa ggagcattag gttattgggc tttaacacca aatattgatc tgtggggaat
    64561 gtggcgttca cgagtatttt tatggactga ttcaacaacc ggtattcgtg atgaaggggt
    64621 gtataacagc cagacgggaa aatacgataa acatcgcgca cgttcttttt tagccgccag
    64681 ttggcatgat gataccagtc gctattctct gggggcatcg gtacagaaag atgtttccaa
    64741 tcagatacaa agtattctcg agaaaagcat accgctcgac ccgaattata cgttgaaagg
    64801 ggagttgctc ggcttttacg cgcagctcga aggtttaagt cgtaatacca gccagcccaa
    64861 tgaaacggcg ttggttagtg gacaattgac ctggaatgcg ccgtggggaa gcgtatttgg
    64921 cagtggtggt tatttgcgcc atgcaatgaa tggtgccgtg gtggataccg acattggcta
    64981 tcccttttca ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc aattgggcgt
    65041 caactatcgt ttaacgccgc aatttacgct tacatttgca ccgattgtga ctcgcggcta
    65101 tgaatccagt aaacgagatg tgcggattga aggcacgggt atcttaggtg gtatgaacta
    65161 tcgggtcagc gaagggccgt tacaagggat gaatttcttt cttgctgccg ataaagggcg
    65221 ggaaaagcgc gatggcagta cgctgggcga tcgcctgaat tactgggatg tgaaaatgag
    65281 tattcagtat gactttatgc tgaagtaaaa aataacgccg gagagaaaaa tctccggcgt
    65341 tttgtctgtg aattactgct gcggaatagc gggtacgacc ggaacattta atgccggcat
    65401 tgcaaacata ccaacgaaat cttccagtgg cattttctgc ccgtttaacg ttatctgacc
    65461 gttagtatat tgcaggctgg tggtgatggt attgtcctgc aaggtggtca gacggaacat
    65521 ctgccccatt gctgatgcac cttcaacttg ctgtttcgcc agttttttcg cttgatcttc
    65581 ctgataacct tccagcttcg ctacctgagt cataaactca gttgccatat ccaccggaat
    65641 ggtcagtttc gcatccagag atttaaccga acgatctact tcctgcgcca gcgtttgcgg
    65701 cgcttcttta gtcgttgccg gatctttcag gaacagcgac agattcagcg cactttcacc
    65761 ctgactgttt ttccagctta gcggcgcgat agtaatcacc ggatcgcctt tcaacatcag
    65821 cggcagggcg ctaaagaagg cttccgtcac tttctcctga taaagttcgg gattgttggc
    65881 aatttctggc tgtgccagca gcgcctgagt ttgcgcgtta tattgctggc taaactgatg
    65941 ccaggcttca ccatcaatct ggccgacttt taaagtcagc ttgccgctgc ccagatcctg
    66001 attctgtacc ttcaggctgt ttagcgagta atccagttgg ctattgatcg ttttaccgtc
    66061 attgaccaga tccgatttac cgctgatctc catgccttcc agcagtgcca gttctttgcc
    66121 ttccactgaa atggtcattt tttccagtga cagtttttgg tttcctacgc gctcaccaaa
    66181 acttgccagc gtgctggaac cgtcggtttt cagattatta aaggtcaact gcactttctg
    66241 gttgtattcg ttaactgcgt ctatccgacc actttgcgcc tccccggaaa gggagatggc
    66301 tttgccgtct ctgtcagcat ttaactggaa ctcgccgccg ctaaaggcga ctttttcatc
    66361 cttttgctcg taattcagtg gcttgagcga aatatcggaa ctggaatcac cgctgtaacc
    66421 aatgcgcgag ttaatctcaa aaggcgtttc accttttgcc atatcaaaca gtggtttgct
    66481 tacttcgtta ttaaccagcg tggtttgaat tgatgccatc gacgggatca ggttcagttt
    66541 tttaagctgg gcaagcggga agggaccatg atcaaccgat tcgttgaaga tgacgctctg
    66601 accgctttta atccacggat tttctttccc ggcaatgggt ttcaccaaca gttgcaactg
    66661 gctgctgaat acgccgcgat gatagttttg ataactcact tccaggttgg attcaggagc
    66721 tgtcagtttg agttgcgcgt tcgcctgcgc gaccatgtct tcgagatggg tttcaatctt
    66781 cttgcctgta taccatgcgc cgcctgtcca gactacgcct agcgcaacaa tgacgcctac
    66841 cgctaccagc gatttattca taatgattat ccataaaatg aaatcaggcg gactggccgc
    66901 ctgaaggtgt tataagcctt taataagctt agcaagagat gttaattttt tcagtaagct
    66961 cttacagctt gttgtaaaca cgcgctaaac ggccgtggcc tttgacagtc accggtgatt
    67021 cgttggcggc aataaacgct gattcacccg gtttaagctg taactgctga gaacctttcc
    67081 acaacgttgc atcgccttcg acgcagaaca aaatggcggc actctgctgg ctaatggtgg
    67141 tttctttatc actaaggtca tgcagcgaga aggcaaaatc atccactgga atcgggaagt
    67201 ccagttctgc accttgtttc accggctggg tcaacaactg gttagccggt ttggcttcga
    67261 atttcacatt ggcaaccagt tccggaatat caatgtactt aggcgtcaga cccgcacgca
    67321 gcacgttatc ggagttcgcc atcacttcca gcgccacgcc ttgcaggtaa gcgtgcggtg
    67381 tttcagcgaa caggaacatc gcttcgccag gattcaattt caccacattc agtaacagcg
    67441 gggagaacag gccgctgtct tccgggtaaa attcagaaat taaacgaatc gtttgccacg
    67501 gttcaccctg ctgactgtcg agcgccgatt ttaaaatcgc cagcgcgcgg gatttttctt
    67561 caccctgcat attcaacagg ctggcgaaca gttcgcttaa acgttcggca ttaggctgtt
    67621 gtaaaaagtg agcaattgcc ggatgtgcac ctgcgactgg ctggagtagg gagacaatct
    67681 cggaaaactc acgaaacgcg ttcatcgcaa ggaaaggcgt cagcgcaaaa accagctccg
    67741 gcttgtggtt aggatcttta tagttacgct cggcggcatc catcgggata cctgcggcat
    67801 tttctttggc aaaaccgatt tcagaattgc gtttgtttgg atgaacctga atggagagcg
    67861 gctgtgctgc gcataatact ttgaacagga aaggcagttc gccaaagcgt ttggcaacgg
    67921 cctctccgag cagagtcgat ttatcactct caatcacatc acgcagtgaa acgatatctc
    67981 cggcggcatt ctgcactcgt gaactgcttt tcggatgtgc gcccatccac agctcggcca
    68041 tcggctggct ggacggattt tccataccat aaagttcagt caacgccgtt ttgctgcccc
    68101 aggcatagtt ttgcactgag ttaatgagtt tttgcatgat caatccctgt tttaatgtgg
    68161 aaattaatcc cactattaaa gcaagaatcc tacgggaagt aacctggagc cgcaaaaagt
    68221 cgtactagtc tcagtttttg ttaaaaaagt gtgtaggata ttgttactcg cttttaacag
    68281 ggcaacggaa cacccgccca gagcataacc aaaccaggca gtaagtgaga gaacaatgtc
    68341 aaacaaaccc tttcattatc aggctccttt tccactcaaa aaagatgata ctgagtatta
    68401 cctgctaacc agcgaacacg ttagcgtatc tgaatttgaa gggcaggaga ttttgaaagt
    68461 cgcacccgaa gcgttaactc tgttggcgcg tcaggcgttt catgatgcgt cgtttatgct
    68521 gcgtccggct caccaacaac aggtggccga cattctgcgt gacccggagg ccagcgaaaa
    68581 tgataaatat gtggcgctgc aattcctgcg taactccgac atcgcggcga aaggcgttct
    68641 gccaacctgt caggataccg gcactgcgat tattgttggt aaaaaagggc agcgtgtatg
    68701 gaccggtggc tgtgatgaag cggcgctggc gcgcggtgtc tataacactt atatcgaaga
    68761 taatttgcgc tactcgcaaa acgcgccgct ggatatgtat aaagaggtga ataccggcac
    68821 caatctgcca gcgcagatcg atctttatgc cgttgatggc gacgagtaca aattcctctg
    68881 tatcgccaaa ggtggtggtt cggcaaacaa gacgtatctc tatcaggaaa ccaaagcgtt
    68941 actaacgccg gggaaactga aaaattacct ggttgagaag atgcgcacgc tgggtacggc
    69001 ggcctgtcct ccgtatcata ttgcgttcgt aattggtgga acttctgcag aaactaacct
    69061 taaaacagtg aaactggctt ccgctaaata ctatgacgaa ctgccaacgg aagggaatga
    69121 gcacggtcag gcattccgcg atgtggaact ggaaaaagaa ttgctgatcg aagcgcaaaa
    69181 tctcggtctg ggtgcgcagt ttggtggtaa atacttcgct cacgacatac gcgtgattcg
    69241 cctgccacgt cacggcgcat cctgtccggt tggtatgggt gtttcctgct ctgctgaccg
    69301 taatatcaaa gcgaagatca accgtcaggg gatctggatc gaaaaactgg aacataatcc
    69361 aggtaaatat atccctgaag agctgcgtaa agcgggagaa ggcgaagcgg tgcgcgttga
    69421 ccttaaccgt ccgatgaaag agatcctcgc acagttgtcg cagtatcccg tttctacacg
    69481 cttatcgctt aacggcacga ttatcgtcgg tcgtgatatt gctcacgcca aactgaaaga
    69541 gcggatggat aacggtgaag ggctgccgca gtacattaaa gatcatccga tttactacgc
    69601 gggtccggcc aaaacgccgg aaggttatgc ctccggttct cttggcccaa cgaccgccgg
    69661 acggatggat tcttatgtcg atcaactgca agcgcagggc ggaagtatga tcatgctggc
    69721 gaaaggcaac cgcagccagc aggtgacgga tgcctgtaaa aaacacggcg gcttctacct
    69781 tggcagtatc ggtggtccgg ccgctgtatt ggcgcaggga agtattaaga gcttggaatg
    69841 tgttgaatat ccggaactgg gaatggaagc catctggaaa attgaagtgg aagatttccc
    69901 ggcgtttatc cttgtggatg ataaaggaaa tgacttcttc cagcagatac aactcacaca
    69961 gtgcactcgc tgtgtgaaat aaacagagcc gcccttcggg gcggtttttt tacatggcac
    70021 gaaagaccaa acatttgtta tcaaatagta aataataagt gagctaaaag ttgcttaacg
    70081 aaagcaaaac agaaagaaaa aattaaacag gtgaggagca ggccatgaat acagtacgca
    70141 gcgaaaaaga ttcgatgggg gcgattgatg tcccggcaga taagctgtgg ggcgcacaaa
    70201 ctcaacgctc gctggagcat ttccgcattt cgacggagaa aatgcccacc tcactgattc
    70261 atgcgctggc gctaaccaag cgcgcagcgg caaaagttaa tgaagattta ggcttgttgt
    70321 ctgaagagaa agcgagcgcc attcggcagg cggcggatga agtactggca ggacagcatg
    70381 acgacgaatt cccgctggct atctggcaga ccggctccgg cacgcaaagt aacatgaaca
    70441 tgaacgaagt gctggctaac cgggccagtg aattactcgg cggcgtgcgc gggatggaac
    70501 gtaaagttca ccctaacgac gacgtgaaca aaagccaaag ttccaacgat gtctttccga
    70561 cggcgatgca cgttgcggcg ctgctggcgc tgcgcaagca actcattccg cagcttaaaa
    70621 ccctgacaca gacactgagt gaaaaatcgc gtgcatttgc cgatatcgtc aaaatcggtc
    70681 gaacccactt gcaggacgcc acgccgctaa cactagggca ggagatttcc ggctgggtag
    70741 cgatgctcga gcataatctc aaacatatcg aatacagcct gcctcacgta gcggaactgg
    70801 ctcttggcgg tacagcggtg ggtactggac taaataccca tccggagtat gcgcgtcgcg
    70861 tagcagatga actggcagtc attacctgtg caccgtttgt taccgcgccg aacaaatttg
    70921 aagcgctggc gacctgtgat gccctggttc aggcgcacgg cgcgttgaaa gggttggctg
    70981 tgtcactgat gaaaatcgcc aatgatgtcc gctggctggc ctctggcccg cgctgcggaa
    71041 ttggtgaaat ctcaatcccg gaaaatgagc cgggcagctc aatcatgccg gggaaagtga
    71101 atccaacaca gtgtgaggca ttaaccatgc tctgctgtca ggtgatgggg aacgacgtgg
    71161 cgatcaacat ggggggcgct tccggtaact ttgaactgaa cgtcttccgt ccaatggtga
    71221 tccacaattt cctgcaatcg gtgcgcttgc tggcagatgg catggaaagt tttaacaaac
    71281 actgcgcagt gggtattgaa ccgaatcgtg agcgaatcaa tcaattactc aatgaatcgc
    71341 tgatgctggt gactgcgctt aacacccaca ttggttatga caaagccgcg gagatcgcca
    71401 aaaaagcgca taaagaaggg ctgaccttaa aagctgcggc ccttgcgctg gggtatctta
    71461 gcgaagccga gtttgacagc tgggtacggc cagaacagat ggtcggcagt atgaaagccg
    71521 ggcgttaatc tgcaacatac aggtgcagcc gtggaatgat caaacgaagc ggctgcgcct
    71581 gtggtttata acggtgctgc acattgtcgg catcgtaatt taacaactca ccaacgtccg
    71641 gcacgcccgc gccattatcc cgattaatca gtgcaatcag tggtgtaggg caggcgtgtt
    71701 ggacttgttt ttgatctcct ttgtaccaga cgcgggcaat cggctgcacc ttcaccggac
    71761 gtttgatttt taacttcgcg ttctgtggca gggcagcgat atcctgatac tctcgctcca
    71821 gttttctttg ccactcctcg cgcgtccacg gtgcgacact gcgtggtgat ttcaggcttt
    71881 tttccagctg tgccaggact tcatcacgat gcaaattctt aatgatatgt ttattagccc
    71941 aaccaaagcg taaagtggcg gggtcgtgca gaacggtgag cgtgcggtaa gcattaaggg
    72001 tgatcagccc cggcaaatga cgatgcaccc attcaaaacg tgccgcggtg gggagttctg
    72061 attcaaccgt gacgatatgc tcgaacgtgg tcttgagttt attgatgtgc tgaatatgac
    72121 tgaccaacgc tgcttgcgaa aggttatcga cctggtaaca caacacgcca ggcagacgga
    72181 cagcggcctt gctgctgcga ttttcggact gttgttgaat aaataaatgg cggaaatgac
    72241 gcaacgccag cgactgcgcg tcgttgccga gatgttgttt tacctcaata cgattaagcg
    72301 gattatgctc atcctctttt tttacctccg gcaaagagaa cacgcgggca accaatagct
    72361 tgtgttgctc aagatgagcg gcaaatgtag ccagctcttg ttccatctgg cgaaaggtag
    72421 tgttgagtcg gtctacgaga tcgtaacgcg ccataatatt aaccacttta gttacaacat
    72481 acttatttta tagcacagtc gtggtgactt gaccatcgtg cgcgtagtgt caggcagagg
    72541 taaattgcgg gatgttatgc cataacggcc agctaaacga gaagcgagca ccacccagtt
    72601 cgctggtgtc acaattaacc gtaccgccca ttgccagtgc tatagagtgg acaattgcca
    72661 gccccagccc gcagccgccg gttgagcgat cccggctggg atcgaggcga acaaaaggtt
    72721 caaagatatg ttcgcggttt tctggggcaa tccctgggcc atcatcctca acaattaatg
    72781 tcgctctatt ccccgacagt agcaggctgg tttcaaccgt tgaatggcag taccgcaggg
    72841 cgttattgag caaattatcc agcacgcgct ccattaagcg catatccaac gccgcataat
    72901 ggccttgcac gagcgttttt atccgtaccg ttttatcggg cgttactgcc tgaatatctg
    72961 ccagatgcgt tgacagccac aacggcaggt ctggttcgct aagatgaagc tcgttttgtg
    73021 ggcgatcgag tcgggcataa gtcagcagct cttcaattaa agcttcaagt tgactgatat
    73081 cacgattcaa cgcctgggat tcggcggcgc tcaggttatc gctcatctcc agtcgataac
    73141 gcaggcgcac taacggtgtt cgcagttcgt gagcgatacc gtcaataagc tgttttttgc
    73201 tggcaatcaa ggcgttgata ttgtccgcca tctggttaaa tgcgatacca agtcgttcaa
    73261 agctcgaacc ctcatcaaag tggatacgct cattgagatg cccatcgcca aatcgttgcg
    73321 ccgctgcttc cagttttaac atatcctgcc agtgcggacg catccagata aacaccggaa
    73381 aggcgaggga aatagcaata aaagcgatca gggcgatatc cagcaatcgc atctgatgga
    73441 gataataaag ataaggaaca ggaccaactg ccagcacgta gtggctgcgc gggatacgct
    73501 gcaaaaacgt gtactgatcg tccagggcga caatttcgcc gccacgcagt cggtgcatgg
    73561 aaatatcatc aagatggtat ttactcagtg gctcgacacg cagatcgaaa gagagattta
    73621 aatccatctc tttcagcgtc ttaccccagt cgtgtggggg gatctcacgc aattcgctgc
    73681 gcatcagata cagcgaactg ttcatcaaat catccagcga ctgtttgccc gcgcgttcgg
    73741 cggtaaattt gtacaccaac ccaaccagca gagacatcac aaggaagcag acaaacaata
    73801 acaggtaaaa ctggataaac agttttttca tcgcttattc ccatgcatga ggcgcaaaaa
    73861 gatagccttt gttacgcaca gttttaatgc gataaggttc tgcggcgtta tcgagcagtt
    73921 tttttcttaa ccgcgaaata gccacgtcca cgctacgatc cagtccgtca taactgacgc
    73981 cgcgtaaatt tttcagcaat gcatcgcggt ccatgatttg cccggcatgg gtagctaatt
    74041 cccacaataa ttcgaaatca gctgtcgaga gcgagatttc agtgttagcc agggtgacta
    74101 cgcggttgat gggatcgatg gtcaacgtgc cgaaatgcag ggctttgtag ggagtcagag
    74161 acgtttcctg aagacctttg gtcagtgtgg cttgctcatt ctgacgcaaa tgcaaacgta
    74221 aacgcgctag caaaacagca gggggcgtcg ttttgagaat atagtcgcag gcacccattt
    74281 ccagtgccag gatgtggttc atatcgctat cgagagaggt tagaagaaca atcggtccag
    74341 accactttgc gcgtaaatca cgacaaatgg tcatgccgtc cttgcctggt agcatgatgt
    74401 cgagtaacac caaatccgga ctttctcgca aaatggtttc ttcggcctgg tcgccgcgcg
    74461 gttctacggt aacctgcata tcatgttttg ccaggtacgc ggcaatcagt gaaccgactt
    74521 ccgcatcatc ttccacaaat acgatagtgt tcataacatt cacggtagat ataaaaacgt
    74581 cacaatacac cgcgctgttt ttacttacta tcaatcgttt ctaaacaagt tttcatcagg
    74641 tattctactg atgacttgtt atggaagtgt taaggtaaaa agatggggct ggtaatcaaa
    74701 gccgcgctcg gtgcgctggt agtgctgttg attggtgttt tagcaaaaac gaaaaattat
    74761 tatatcgccg ggctgattcc acttttcccg acctttgcgc ttatcgcgca ttatattgtt
    74821 gccagcgaac gcggcattga agccttacgc gcaaccatca tttttagtat gtggtcgatt
    74881 attccctatt ttgtttacct ggtgtcgctg tggtatttca ccggaatgat gcgcttgccc
    74941 gccgcttttg ttggttctgt tgcgtgttgg gggataagcg catgggtgtt gattatatgc
    75001 tggataaagc tgcattaacg cagatgacga ccgccatcaa gtgggaaact gcgtccggtg
    75061 acaaagcagc tggtaagtaa gtaatcgacc aggtcgatca cttctttctc gccaggcgcg
    75121 gttttcatca gtgatttatt cagcgcctgt tgtcgatatt cggcatcatc atgttcatta
    75181 aacaggatca gcgatggcgc aatagaattc actttcactt ccggtgccag cttgcgggca
    75241 aacgagcggg tcatattatc cagtgccgct ttgcttgcag catacgcaac atgtttgtcg
    75301 ctaccgcgct ccaccacata atcggtaaag tgaatgatat cgctggcggc gtgtccgtgc
    75361 ccacgcagta atctttccag cgcatggttg agcaggtatg gggtattaac gtggatctgc
    75421 atcatgcaag ccagtacgtc ggccagtggt gtacctggtt tttccgccat ccacgcactg
    75481 gcgttatgca aaatagcacg cagaccatgg gtgcttttta gtacttcatc ggcaaacgcc
    75541 atcacaccgt cgttggtcga aaaatcagcc tgaatacact gcgcacctgc cttaatcagt
    75601 ccatcaatgg ctggatagtg cgtccgatag ctgacaatca ccggttgctt ttgattaatg
    75661 aaatgccagg cgagggcgag gccgatgcga cgacctccgc cagtaattaa tattggcaag
    75721 ggctgggctt tacccatcgt tatctccttt gctatccaac gatgggatga acgttatccc
    75781 accagcatcc aggtcgccgg aactgaggca attaacagca tgccaatcag caccatttcc
    75841 tgacggttca gtacattatc atgtgtatgc gttttgcgcg catacaggaa aaccagcagc
    75901 cccggcgcat acagaacaac ggataacagc aagtgcattg gaccagaggc atacaataac
    75961 cataagccat aaatgcaggc accgacacct accgctttat gcagtggacg ggttgcgatt
    76021 ttcagcaaga acgcgccgac caggaaatag ggcaccagaa tcatttctga ggcgatggtc
    76081 agcaacgtgt tgtaatcgga accggtcagc cagatgagta ccagacaaat ttgcacgcag
    76141 atgttggtca accacagcga ggcggatggc gcagcttgtg cgttctggcg cgcaaaaatg
    76201 cgtgggaatg ctttatgagt ggcagccagg aacggaactt ctgccgccat gatggtccag
    76261 cttaagtacg cgccgcaaac ggaaacgatc aaaccggcag cgatgatgat ttcgccccat
    76321 ggtcccatca tttccaccat cagaccggcc attgacgggt tacgaatttc agccagttca
    76381 ggacgtgcca ccacgcccag cgaaagcagc gttaccagca agtaaacgcc cagagcggag
    76441 agaaccgcca gcagtgtcgc tttgccaaca tcacgtttat tacgcgcacg cgcagaaaca
    76501 accacggcac cttccacacc aatgaaaacc cacagggtga tcagcatggt gtttttcacc
    76561 tgttcccaca cgggtacgcc aagtgcaagt ccggtgaagt cgagcttgaa ggtatccagt
    76621 ttgaacatca tcatcgccag cacaacaaac agacccagcg gcaacaattt tgccagtgtc
    76681 gccaccaggt taatgctggc agcggtttgc accccgcgca ggatcaaaaa gtgaacaatc
    76741 cacaataaag ccgatgcacc gactatcgat tgccaggtat taccatcgcc gaacaggcgc
    76801 aattccggcg tgtccgtaaa aaagcttaac gcggaaaaaa cgatcaccag ataggagacg
    76861 ttggcgatga ctgcgcacag ccagtatccc catgcggaac aaaagccgat tagctcgcca
    76921 aacccttcgc gggcataggt aaagataccg ccgtcaagtt cgggacgaat gcgcgtgagg
    76981 atcagcatgg caaaggccag caataaaatg ccagcgccag taatacccca gccgatgagc
    77041 agtgctgccg ggctggcaac tgccgccata ttttgcggca gactgaaaac acccgcgccc
    77101 agcattgagc ttaataccag cgcggtgagt gcgctcagtc ccagtttctt ttccatcgat
    77161 atccagtgat taaaacgtga taagaagaat aaatattagg gcaaaacgca taaaaatgga
    77221 gtttgccact gaagcgcggg attttacgaa gagatgtgtc tgcatgcaat ggtcgcgggg
    77281 aaatttgtga ctaaaaatgc gaaaggcggc atcaagccgc cttataggag taactgaata
    77341 gttatttata cagatctgcg ctgacggtca ggttattacc acgttcctgc cactggcggg
    77401 tgatgtggta atacttagca cctttcttcg cggcacgttt cgcaacctga taggagactt
    77461 cggtcatgtt gccgtagttg ccagagaatt tgatgctgtc gaacgggacc atcatcgctg
    77521 ctgtcgcttt gttcagttct tcgactttag tgccgtcagg aagcgtgaca gtgtaacgtc
    77581 cgccttttga tgactgggtt tcaaagaaac gaccgacttc agaacttggt gatgcggtag
    77641 tcgcaacacc tgggatctca actttcttcg cggcttcgcc accggcagcc agagctgcac
    77701 gtcctgcttc ggaatctgcc gggatcacat ccgggctctg gacgatacgt ttcttagcat
    77761 cttttttata gatgaatgca gtaatacgct ggttgccgcc ctggttggca tcgatttgac
    77821 gaacaatgta gaaagagtag gcacctttcg cttttgctgc tttggtgatg gcatcattga
    77881 cttctggctg gctacgatag aagccctgga cggtgaccgt gtcaaacggt tcaatcagaa
    77941 cagcctgatc tttcggcagt tcgacaacac cgttaattac gcgattactt gtttcttctg
    78001 ctttttcagc atcggcttta tagaggtcag cgaccacacg ccagttaccg ctgttaccaa
    78061 aatcagaagt gtcgacaaca taaaaagagg cggcaccttc tttatctgcg cgacgagaaa
    78121 cggctttcac cgcttcgcca atagcattaa aacgaccggt aaccactaca cggtcaaaag
    78181 gtttaaccgc tgccgcttgc tccggtgtca gttctgttgc tgcgttaacg gagaatgccg
    78241 tagcagaaag cagtgccgac gccaggaggg tgttcttaag cttcataaaa ataatccttc
    78301 gccttgcgca aaccaggtac tggtattgtt attaacgaga aacgtggctg attattgcat
    78361 ttaaacggtg taactgtctg cgtcattttt catatcacat tccttaagcc aattttaatc
    78421 ctgctcaaat gaccgtctat gcttaaaaaa cagccgtatc agcatcatta ctactgaagc
    78481 aactgaattg tataagttaa tttaatgtta agtagtgatt cgtgccgggg cgatgtctcg
    78541 ttttacccga ccgtcgaaga caattatcag tctttatccg gcgttctaag gtgtttatcc
    78601 cactatcacg gctgaatcgt taatattttg cgagttcacg ccgaaatact gatttttggc
    78661 gctagatcac aggcataatt ttcagtacgt tatagggcgt ttgttactaa tttattttaa
    78721 cggagtaaca tttagctcgt acatgagcgg cttgtgtggc tcctgacaca ggcaaaccat
    78781 catcaataaa accgatggaa gggaatatca tgcgaattgg cataccaaga gaacggttaa
    78841 ccaatgaaac ccgtgttgca gcaacgccaa aaacagtgga acagctgctg aaactgggtt
    78901 ttaccgtcgc ggtagagagc ggcgcgggtc aactggcaag ttttgacgat aaagcgtttg
    78961 tgcaagcggg cgctgaaatt gtagaaggga atagcgtctg gcagtcagag atcattctga
    79021 aggtcaatgc gccgttagat gatgaaattg cgttactgaa tcctgggaca acgctggtga
    79081 gttttatctg gcctgcgcag aatccggaat taatgcaaaa acttgcggaa cgtaacgtga
    79141 ccgtgatggc gatggattct gtgccgcgta tctcacgcgc acaatcgctg gacgcactaa
    79201 gctcgatggc gaacatcgcc ggttatcgcg ccattgttga agcggcacat gaatttgggc
    79261 gcttctttac cgggcaaatt actgcggccg ggaaagtgcc accggcaaaa gtgatggtga
    79321 ttggtgcggg tgttgcaggt ctggccgcca ttggcgcagc aaacagtctc ggcgcgattg
    79381 tgcgtgcatt cgacacccgc ccggaagtga aagaacaagt tcaaagtatg ggcgcggaat
    79441 tcctcgagct ggattttaaa gaggaagcgg gcagcggcga tggctatgcc aaagtgatgt
    79501 cggacgcgtt catcaaagcg gaaatggaac tctttgccgc ccaggcaaaa gaggtcgata
    79561 tcattgtcac caccgcgctt attccaggca aaccagcgcc gaagctaatt acccgtgaaa
    79621 tggttgactc catgaaggcg ggcagtgtga ttgtcgacct ggcagcccaa aacggtggca
    79681 actgtgaata caccgtgccg ggtgaaatct tcactacgga aaatggtgtc aaagtgattg
    79741 gttataccga tcttccgggc cgtctgccga cgcaatcctc acagctttac ggtactaacc
    79801 tcgttaatct gctgaaactg ttgtgcaaag agaaagacgg caatatcact gttgattttg
    79861 atgatgtggt gattcgcggc gtgaccgtga tccgtgcggg cgaaattacc tggccggcac
    79921 cgccgattca ggtatcagct cagccgcagg cggcacaaaa agcggcaccg gaagtgaaaa
    79981 ctgaggaaaa atgtgcctgc tcaccgtggc gtaaatacgc attgatggcg ctggcaatca
    80041 tccttttcgg ttggctggca agcgttgcgc cgaaagagtt tcttgggcac ttcaccgttt
    80101 tcgcgctggc ctgcgttgtc ggttattacg tggtgtggaa tgtatcgcat gcgctgcata
    80161 caccgttgat gtcggtcacc aacgcgattt cagggattat tgttgtcgga gcactgttgc
    80221 agattggcca gggcggctgg gttagcttcc ttagttttat cgcggtgctt atagccagca
    80281 ttaatatttt cggtggcttc accgtgactc agcgcatgct gaaaatgttc cgcaaaaatt
    80341 aaggggtaac atatgtctgg aggattagtt acagctgcat acattgttgc cgcgatcctg
    80401 tttatcttca gtctggccgg tctttcgaaa catgaaacgt ctcgccaggg taacaacttc
    80461 ggtatcgccg ggatggcgat tgcgttaatc gcaaccattt ttggaccgga tacgggtaat
    80521 gttggctgga tcttgctggc gatggtcatt ggtggggcaa ttggtatccg tctggcgaag
    80581 aaagttgaaa tgaccgaaat gccagaactg gtggcgatcc tgcatagctt cgtgggtctg
    80641 gcggcagtgc tggttggctt taacagttat ctgcatcatg acgcgggaat ggcaccgatt
    80701 ctggtcaata ttcacctgac ggaagtgttc ctcggtatct tcatcggggc ggtaacgttc
    80761 acgggttcgg tggtggcgtt cggcaaactg tgtggcaaga tttcgtctaa accgttgatg
    80821 ctgccaaacc gtcacaaaat gaacctggcg gctctggtcg tttccttcct gctgctgatt
    80881 gtatttgttc gcacggatag cgtcggcctg caagtgctgg cattgctgat aatgaccgcc
    80941 attgcgctgg tattcggctg gcatttagtc gcctccatcg gtggtgcaga tatgccagtg
    81001 gtggtgtcga tgctgaactc gtactccggc tgggcggctg cggctgcggg ctttatgctc
    81061 agcaacgacc tgctgattgt gaccggtgcg ctggtcggtt cttcgggcgc tatcctttct
    81121 tacattatgt gtaaggcgat gaaccgttcc tttatcagcg ttattgcggg tggtttcggc
    81181 accgacggct cttctactgg cgatgatcag gaagtgggcg agcaccgcga aatcaccgca
    81241 gaagagacag cggaactgct gaaaaactcc cattcagtga tcattactcc ggggtacggc
    81301 atggcagtcg cgcaggcgca atatcctgtc gctgaaatta ccgagaaact gcgcgctcgt
    81361 ggtatcaacg tgcgtttcgg tatccacccg gttgcggggc gtttgcctgg acatatgaac
    81421 gtattgctgg ctgaagcaaa agtaccgtat gacatcgtgc tggaaatgga cgagatcaac
    81481 gatgactttg ctgataccga taccgtactg gtgattggtg ctaacgatac ggttaacccg
    81541 gcggcgcagg atgatccgaa gagtcctatt gctggtatgc ctgtgctgga agtgtggaaa
    81601 gcgcagaacg tgattgtctt taaacgttcg atgaacactg gctatgctgg tgtgcaaaac
    81661 ccgctgttct tcaaggaaaa cacccacatg ctgtttggtg acgccaaagc cagcgtggat
    81721 gcaatcctga aagctctgta accctgacgg cctctgcgga ggccgtcact ctttattgag
    81781 atcgcttaac ataacggcga tgctttgacc tcccgctgtt tgttcaagcg caattttgac
    81841 aataattgtt aacggcacgg aaagcagcat acccaccggt cctaacaacc atccccaaaa
    81901 aatcaacgac aaaaatacca ccaatgtgga aagccccagc ccacgtccca tgatgcgcgg
    81961 ctccagaata ttgccgaaga ccagattaat cagcagatat cccgccagca ccagcaacgc
    82021 ttcgtagaag ccattaaaca ccagtacctg agcgataggg gggattgccg cgagaactga
    82081 accaatatta gggatgtaat taagcgcaaa ggccagcaat ccccagacaa aagcgaagcg
    82141 aacatcgagt gcggcgagca tcgcccaggc gaccaggccg gtgatgatgc tgatggctgt
    82201 tttcagcacc agataatgag aaacactgtc aattgcacgt tgaatcgccg ccatcccttc
    82261 aaccggacgc gccatcattt gctgaaattt tccgggcaat tgtggcactt cgagcagcat
    82321 aaacagcacc gtcagcagca ataaaaatat tgatgacatg gcattagata actgcgtcaa
    82381 taagttggtg agcaacgtca tcgccgcgtt cggatcaatg taatgcgcca gctggtcaac
    82441 tgagacgtca atccctacgc gttgcaacaa cggctcaaga gcttgcagcg gcgtcataat
    82501 agagttgcga tattgcggta acgtccgcgt caactcgttg agcgcggaac ccagataagc
    82561 taatagcaac accatcgcca tcacgatgat ggtcatcaaa atcgacaccg ccagtacacg
    82621 cggcactcgc cagcggacca tgtgttgcac cagcgggtta agaataacag caataaataa
    82681 tgcgagaata aacggcacga tgatctcggc ggcaaaacgg ataccgcaga gaataatgac
    82741 cagcattccc aacataatga cgatttttag gccattgagc gtgatgatcg gctttgccat
    82801 gcttgtcccc gttttttctt cctttgctaa taataatgtc tttttcgctc cgtcatcata
    82861 acgaaaatca aaccgctcag gtaaagaagt gaaaatattt tgagttaatt cttaagctat
    82921 gatacaaatc aggcgtgttc aactaccgag gacaattatc atccgcgatg acgagaagca
    82981 acactgcgga taattgtaat attatggaca atttgttcag gacgttattt tcaacattta
    83041 cccatcttcg tacctcatcc actatcttgc tcgtaggtga gcagcactgg cgcaacgcgc
    83101 tttagtcctt tcttctgcct aagcttgccc tatgcgcggg gcttgaccac tttactttgg
    83161 tttcgctgaa ttaagcgaaa attaaaataa ttctcttgca ggagaaggac aatgtatatt
    83221 tattggattt tattaggcct ggctattgct acagaaatta ccggtacgct gtcaatgaaa
    83281 tgggcgagcg tcagtgaggg aaatggcggc tttattttaa tgctggtgat gatttctctg
    83341 tcgtatatat ttctctcttt cgccgttaaa aagatcgcct taggcgtagc ttatgcgctg
    83401 tgggaaggta tcggtatttt atttattacc ttgtttagcg ttttgttatt cgacgaaagt
    83461 ttatcgctga tgaaaattgc cgggttaacc accctggtcg ccgggattgt gttgatcaaa
    83521 tcaggtaccc gtaaagcgcg taaacctgaa ctggaggtga accatggcgc agtttgaatg
    83581 ggttcacgcc gcctggctgg cattggcaat cgtgctggaa atcgttgcta acgtcttttt
    83641 gaaattttct gacggctttc gtcgcaaaat atttggcttg ctctccctgg cggcggtgct
    83701 ggctgccttt agtgcgcttt ctcaggccgt aaaagggatc gacttgtctg tcgcttatgc
    83761 attgtggggc gggtttggta ttgccgccac gttagccgca ggttggatct tgtttggtca
    83821 acggttaaat cgtaaaggct ggattggcct ggtcttgctg ttggctggaa tgatcatggt
    83881 gaaacttgcc tgatgaacac gctgcccgca ctgtcgggca gcgtttgaac attatttttg
    83941 cgacagttga tccagcttgt cgcgaaaacc ggtaacagaa atggcccggt tatcggcgcg
    84001 ccagcgatct ttcgcggcag gagccgaact ttgcacccca attaattgcc agccgtcatc
    84061 ggtatgcaac atcaaaggcg aaccgctgtc acccggcaag gtatcgcact gatgtgacat
    84121 caccgacgtt tgcgcccagc cagtcacttc acagttttga tgactgtata acgtatcgag
    84181 atgatcttca ggatatcccg cctgagtcac tttacgacca gccgatttta atgcggcagt
    84241 aagcgcggct ttatctccct caaataacgg caacggtgta atgccagaag ggggattacg
    84301 cagcacaatc aagccgaagt cccatggtgc agctgcggga ggaacaatcc aaccatcccc
    84361 atctgctttt aaccgctttc ccagtgtcgg atcaacgcgg ccttctatgt cgtggatgtc
    84421 atagcgccaa agacctttat ttgacacaaa acgcagcgct accgccttat ccgctttacc
    84481 cttaggcggc gtcaataaac agtgtcctgc cgttaatgcc agattgggtg caatcagcgt
    84541 cgccgtacat aaattgccgc tggccgtttc cagttgccca accgcatccc acggtgattg
    84601 ggtcgtgtca ttcactggca cacgatcatc atgaccaaaa aacagggtgc tgacctcatc
    84661 gtttgccgat ttggcaacgt ctggtttatc tgcaaacaca aaagcagacg tcaaactaat
    84721 tgcacccaac actacagcaa tggttgtacg catatcacac tctggtgggt aattatgatt
    84781 attaaaagca aaccctcata aatactatag acgggacggg atgagagtgg gagtaaaatc
    84841 agataactac agtcaggaaa gataaaaacg ggcggcaatc agtgcgcata aaataaggat
    84901 gataagaatg aactcaaagc gataacggcc caccatacta ccctccgcta aaggcggtgc
    84961 taaacgttac ctgcttagca ccgtaatttt caggcgcgag ggggcgcgcc agcattactg
    85021 ttgaaaactt acgctgcggg ttgtgcagca ggttttgccg gttgctgatg gctgtgtttc
    85081 ccggcgtgtt tcttcgctgc ctgcgctttt tgttcagggg ctttctgttc agctttcgta
    85141 tttttatgat gctttttagc cgcctgcgct ttctgggcag gtgctgcttt atgctgtttt
    85201 ttatgatgtg tcgttttcgc cggcgctgct ttggtggtcg tcgcagtcgg agcaggtgtg
    85261 gtcgcagtct ctgcagcaaa ggcggcagaa gacagaccca tagcagcggc aacaaccaga
    85321 gctaatactt ttttcattgt cataccctca atttgttttt tcatttaacc ccactgcggg
    85381 gccgttgaaa taactatatc cctgggaatg gcagacttcc gtgagtggtt ggtttcagcg
    85441 tgtaacgata tgtacaaacg ctgaataaat tacagcgttg ataataacgt attgtgactt
    85501 aagggaaatt tagctaccaa taatagtagt cttgatcggg ttaactattt tactacttac
    85561 aaagcggtag aataactgcg catcagtaat aaatgacaca cagcaaaatg aatccgttta
    85621 tttgggtact tataatcctg atgacgctag acgcgctgcg ggaattggct ggcgcttcgt
    85681 ctattttagg atggctatta acgttggttt gagcaagcaa taagtccgga cgtgtagtta
    85741 ccgcagtccg ggcttatttt tcaggcgtgc agacgacgat gcaaacgcgt cccgaccagc
    85801 agagcaatga ccagcatcag agcaataaat gcgccgacgc cgttccagcc atagttatgc
    85861 cagaaaacac cacccagcgt cccggcaata ctcgacccca gatagtaact gaacagatac
    85921 agcgaggagg cctggccttt agcgcgtttt gcgcgggggc caatccagct gctggcaact
    85981 gagtgggctg cgaagaatcc tgctgagaag agtaacattc cggcaaagat cagccacagc
    86041 gagctgaata aggtcatcag taaaccaaac agcataaccc ccgtcgaaaa caacatcacc
    86101 ggaccgcgcc cgtagcgggt ggtcatggtt ccggctttgg gtgagctcca tgtaccggtc
    86161 aaataagcca gcgataataa gccaaccacg gcctgactga catgccaggg ggagagcatc
    86221 aaccgatatc cgatgtaatt aaacagcgtg acgaacgacc ccatcagcaa aaagccttct
    86281 gcgaacaata acggtaatcc ccggtcacgc cagtgcagac gaaagttgat aaacaacgtc
    86341 ttaggtcgca gcgaagtcgg gcgaaaatgg cgtgattcag ggaggatttt ccagaacatc
    86401 aacgccgagg ctagcgcgaa acaaccgatt gccgccagag caattcgcca gttgaaaaag
    86461 tccgtgaaga caccactaat taagcgtccg ctcatgccgc caattgagtt gccgctgata
    86521 tacaacccca tcgaaaaggc cacgaaactg gggtggattt cctcgctaag ataagtcatg
    86581 ccaacagctg ccacgccact taacgaaagc ccaatcaagg cgcgcataat caaaatgccg
    86641 tgccagctgg tcatcattgt cgaaagtaac gtacaaatgg aggccaacag tagcgccgtg
    86701 accatcactg gtttgcgacc aatggcatcg gatagggggc cagtaaacag caaaccaata
    86761 gccaacatcg ccgtggaaat ggacagtgaa atactactgt tcgcgggcgt taagccaaac
    86821 tcctgcgaaa gcaccggaag gataggctgc acacaataga gaagtgcaaa tgttgccagt
    86881 ccggcagaga acagcgccag ggtgacgcgc ataaattgcg gcgtaccgcg tttaataaat
    86941 tgatttggct gagaaatgct ttgcttgtca gtgtcgcttg ccggagcgcc atcaacagtt
    87001 gtagtacggc tcacttgaaa tccttgctaa atatgcctgt ggatcaggct tatacatagg
    87061 ttaggaaaat cgaattgttc tgtctaatat attaataatc tcaaataaga tgttttaaat
    87121 atgaatattg aacttcgtca tctgcgttac tttgttgctg ttgcggaaga gctgcatttc
    87181 gggcgcgccg ctgcccgcct gaatatttcg caaccaccgc taagtcagca gattcaggca
    87241 ctggagcaac aaattggtgc ccgactgctg gcacgaacta atcgcagtgt attgctgacg
    87301 gcagcaggaa aacagtttct tgcagatagt cggcaaatcc tgtctatggt agatgacgct
    87361 gccgctcgcg ctgaaagact gcatcagggg gaagcggggg agttgcgcat tggttttact
    87421 tcgtccgctc cttttattcg ggcggtgtcc gatacgttat cgctgtttcg ccgtgattat
    87481 cccgatgtcc atttacaaac ccgcgaaatg aacactcgtg agcaaatcgc tccgctcatt
    87541 gaaggaacgc tggatatggg attgctgcgt tacacagcgt taccggagac gcttgaacac
    87601 gcagtcatcg tccatgaacc gcttatggcg atgatcccgc acgatcatcc cctggcaaat
    87661 aacccgaatg taacgctggc tgaactggcg aaagaaccct tcgtcttttt tgatccgcac
    87721 gtcgggacag ggctgtatga cgatattctc gggctgatgc gacgttacca tttgacgccc
    87781 gtcatcactc aggaggtggg cgaggcaatg accatcatcg gtctggtttc cgccggtctg
    87841 ggtgtttcaa ttttgcctgc ttcatttaaa cgtgttcagc tcaacgaaat gcgctgggtg
    87901 ccgattgctg aagaggatgc ggtttctgaa atgtggttgg tctggccgaa acatcatgaa
    87961 caaagtccgg ctgcgcgtaa ctttcgtatt catctgctga atgctctcag gtgagggaaa
    88021 tttcagcgaa aaagcccgaa aaatgtgctg ttaatcacat gcctaagtaa aaatttgacg
    88081 acacgtattg aagtgcttca ccatagccta cagattattt cggagcgcga aaatataggg
    88141 agtatgcggt ggttgctgaa aaccagcctg ggcacattga tcaaataaag cagaccaacg
    88201 cgggcgcggt ttatcgcctg attgatcagc ttggtccagt ctcgcgtatc gatctttccc
    88261 gtctggcgca gctggctcct gccagtatca ctaaaattgt ccgtgagatg ctcgaagcac
    88321 acctggtgca agagctggaa atcaaagaag cggggaaccg tggccgtccg gcggtggggc
    88381 tggtggttga aactgaagcc tggcactatc tttctctgcg cattagtcgc ggggagattt
    88441 tccttgctct gcgcgatctg agcagcaaac tggtggtgga agagtcgcag gaactggcgt
    88501 taaaagatga ctcgccattg ctggatcgta tcatttccca tatcgatcag ttttttattc
    88561 gccaccagaa aaaacttgag cgtctaactt cgattgccat aaccttgccg ggaattattg
    88621 atacggaaaa tggtattgta catcgcatgc cgttctacga ggatgtaaaa gagatgccgc
    88681 tcggcgaggc gctggagcag cataccggcg taccggttta tattcagcat gatatcagcg
    88741 catggacgat ggcagaggcc ttgtttggtg cctcacgcgg ggcgcgcgat gtgattcagg
    88801 tggttatcga tcacaacgtg ggggcgggcg tcattaccga tggtcatctg ctacacgcag
    88861 gcagcagtag tctcgtggaa ataggccaca cacaggtcga cccgtatggg aaacgctgtt
    88921 attgcgggaa tcacggctgc ctcgaaacca tcgccagtgt ggacagtatt cttgagctgg
    88981 cacagctgcg tcttaatcaa tccatgagct cgatgttaca tggacaaccg ttaaccgtgg
    89041 actcattgtg tcaggcggca ttgcgcggcg atctgctggc aaaagacatc attaccgggg
    89101 tgggcgcgca tgtagggcgc attcttgcca tcatggtgaa tttatttaac ccacaaaaaa
    89161 tactgattgg ctcaccgtta agtaaagcgg cagatatcct cttcccggtc atctcagaca
    89221 gcatccgtca gcaggccctt cctgcgtata gtcagcacat cagcgttgag agtactcagt
    89281 tttctaacca gggcacgatg gcaggcgctg cactggtaaa agacgcgatg tataacggtt
    89341 ctttgttgat tcgtctgttg cagggttaac attttttaac tgttctacca aaatttgcgc
    89401 tatctcaatt tgggccagga aagcataact tagactttca aggttaatta ttttcctggt
    89461 ttatatttgt gaagcataac ggtggagtta gtgatgctga agcgtttctt tattaccggt
    89521 acagacactt ctgtagggaa aacggtggtt tcccgcgcat tgctacaagc gttagcctcc
    89581 cagggaaaaa cggttgcggg atataaaccc gtagcgaagg ggagcaaaga gacacccgaa
    89641 gggctgcgta ataaagatgc cctggtgttg cagagtgttt caaccatcga actgccttat
    89701 gaagcagtta atcctatcgc gttaagcgaa gaagaaagta gcgtggcgca cagttgccca
    89761 atcaattaca ccctcatttc aaacggcctg gcaaacctga ccgataaagt cgatcatgtc
    89821 gtggtagaag ggactggcgg ctggcgcagt ctgatgaatg atttgcgtcc actctctgaa
    89881 tgggtggtgc aggaacagtt gccggtgttg atggttgtcg gtattcagga aggttgcatt
    89941 aaccatgcac tgctaacagc tcaggcgatt gccaacgacg ggctgccgct cattggctgg
    90001 gtggctaacc gaatcaaccc aggactggcg cattatgcgg aaatcattga tgtgctgggt
    90061 aaaaaacttc cggcaccgct cattggtgaa ctgccttatc tgccgcgcgc cgaacagcga
    90121 gaactggggc aatacatccg cttagctatg ctgcgcagtg tgctggcggt agatagagtc
    90181 acggtgtaac gtccgcgaaa ttaccgacgc aatgacgcag gcaatcaata aaccggggag
    90241 tagctgatac tccctggtca tttcacatat catcaacgtc gacataatcg gcgcgtgcgt
    90301 ggttgccgcc aacagtgtcg ccatcccggt caatccgagt aaaagcgtaa tttcttcgcc
    90361 atcagggaac cataatccca ggctacgacc atacaacatg ccaatggcaa gaccgataaa
    90421 tagcgtcggt gtaaagacac caccgggtgc gccggaaccg ctactcgcca gcacggcaca
    90481 cagtttacaa aggaagatcc cggcaatgat cattaacagt ggtggggcgg ttaaaaagga
    90541 ttgtacggtg ctatagccgt tgccccacac tgcaggtgtg aacagggaaa gcagacccac
    90601 gatcaacccg cctagtgcca gttgccaggg cggcgcaagt ttgagactca caaatccacg
    90661 atgacaggcg ttcattaacg ttaacaacag tggtccgcac agacctgcca gcacacctgt
    90721 actgataatc agcgcatagt cacgagcctg aaccgtcact gagagttgta cgttgtagag
    90781 taacgcgtcg ctatgattaa tcagattgct aaccagcaat gccacgacgg cggaaataat
    90841 caccgggccg agagaggcca acatcatagt gccaaacagc acttcggcta taaataaact
    90901 gccagcaagc ggggcacgat aggccgcagc cattcccgcc gcggccccac aggcgatcca
    90961 taatttccac tcctggcgtg gcgtaaaacg ttgggcaaaa caggaggcgg caagggcagc
    91021 taaaagaatc atcgcacctt cgcgaccaat tgcactgccg ctggttacta ccagcagaga
    91081 ggcaagcgat ttaaccaggc ttgctgcgta atcgaactgt ccatcggttt gcaacgcttc
    91141 catgtaatcg gtcggcgcat gagggcgttg ttgggtaaat ttctgccagc ccatcagcaa
    91201 caaacccgcc gccagtccgc cgagcgccgg agttagcaac cgtcgccagg gggaaaggtt
    91261 tgtcgctgca ttgaccagac tgccggagtc attattgagg aacaaccact ccagtagcag
    91321 catcgcatga cgaaacccgg caacggcaaa ggccgcgaga ataccgacga ctgttgcgat
    91381 aagcagacgg cggaacatag tgcgcagatc cggatagatg tgtagatgat gcatgagcag
    91441 aaatgggcaa tgaaacacgg gcgactattt tgcggcaaaa gaggccagcc gcaaaagcag
    91501 tgcgtgcttt aagagataaa aaaacgtgac actgtaacct attattgccg gattgcgcgt
    91561 aatcgtcacc atccggcaat attacggtga tcctatcgaa acagcggttt aaccgcgaca
    91621 ggaattaaca gttgtgattg ccattgcgcc agcgttaagc gagccagttc acccagtgca
    91681 cggtagaaag ggtgttctgc tttttcgata aaaacatcaa gaaaacgtgt tgaccacgga
    91741 aaaaggtgcc atgccagcaa ttcttcgcat tctgtctggc gaccattctc cgccaaccac
    91801 gcagccatca acagcagtga accaaaatga tcttccggtt cgttttgctt catttcaaac
    91861 tgaatgcctt tctcgcgcat ccactgacga agtgccaatg ttgaatcgcc aaacagcaca
    91921 gattcgcgat ccagccagac cgacccccac ggcggagacg gcagtgccca cgggccgaca
    91981 aacaaacgct gccaggcctg ggcgtgagtc tcttcacact gtgtctgaaa agccgtcacc
    92041 agcggcgcta atgacgcttc tggtagaggc cactgagttt cccagccgtc actggtgagt
    92101 accgcaacca gaggtgcggc ttccgcactc tctggagcgt aataaaacag cgcgcccaac
    92161 acgcgcgccg cgacagaaaa attatcttgc tgtgaaaaat gggtcattcc taacatcctg
    92221 aaatgtgcgg gttgccccgc accaatttgt taacctgcaa tagccattcc tactgtcatg
    92281 tgcaggccat aaaagagcac acgcccgatc atctcaccgc caagtatgag gattagcccc
    92341 agaataagtc cggcaacgtg aggttcacgg cgacgaatga gcgggcaaag ccagcatcca
    92401 agacctgcgc ataacaatac cacacgccag acctgtaagg aggcgtaatc tggtaccagt
    92461 gcgctggctt gttgcacgga tgagtgaata gacgccagag acaaaccttg cagcacaatc
    92521 accccggcac aagcgatcag cgccagaacg ctaatgatgg caaatggtgt ggtattaaat
    92581 gtaacgcggg ctgcccgcag aattgcggca gcaagtattg gaccgctcaa caataccgtc
    92641 aggaagaaag ccagcgtagt gtaaccgtta tgccaggttg gcacagtgtc gatttgatac
    92701 acgcaggtca tcatccagac gaaaatgacg ccgagcgcca tgctgacaag taaccagagt
    92761 ttccccaacg cttgcggcat tttaccgatg accgccacca gccaccatag tccgccaacg
    92821 gcaaaaaata tggaaccggc agcaatctcg ttactcagac cagaagctcc gattcgattc
    92881 agcgagttaa acgcccgcag tggcgatccc aggtgcatga cagaggcaat aaacccgatg
    92941 cccatcaaca gccagaggaa aaacatgcca cggacaatac gctgacggtc tgcgtcattt
    93001 ttggcggcga accagccaat tccgctaaca atcagcgcgc ctactacgca ttgacccaat
    93061 acggtaaaga taaccagtgg ccattcatgc catccatttc ccattttaca cttcctccgg
    93121 attagccaga taaccagtgg tatcgccggt cgggcggctg ttggcgttag gtttgataac
    93181 gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg acggcagcca gagtgccgtg
    93241 cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca cgcagcgggc aggattcgac
    93301 gcatatgggt tgtttcccct cggcgacgcg cgaataacaa ccatcgcact tcgtcatgtg
    93361 ccctttttca gcattgtact gtggtgcgcc gtacggacaa gccatgtggc agtagcggca
    93421 gccgatacaa acatcttcat caaccaccac aaagccatct tcacgcttat gcattgcgcc
    93481 gctggggcag acttttgtac atgcggggtc atcgcaatgg ttacaggaga tggagagata
    93541 ataagcgaac acgttctggt gccagacgcc attatcttcc tgccagtcgc cgccagcgta
    93601 ttcataaata cggcggaaac tgacttccgg gccaagatct ttaaaatctt tgcacgccag
    93661 ttcgcaggtt ttacagccag tacagcggct ggaatcgata aaaaatccat attgtgtggt
    93721 catgggctac tccttaaacc ttttcgatct ggacaagatt gctgtgcgac gggtttccct
    93781 ttgccagcgg tgaagggcgg tgagaggtca gaatattgat actgccgccg tgatcgaccc
    93841 ggtcaccaaa catatccgct ttaagccacg caccttgccc gatggcggta acgccaggca
    93901 gaatacgcgg agtcactttt gcggcaatca gcatttctcc attattgtta aatacccgca
    93961 cggtatcgcc atgacggata ccgcgtgcct gagcatcaat ggggttgatc cacacctctt
    94021 gtgggcaggc ctgctgtaac acatcaatat tgccgtagct ggagtgggta cgcgctttgt
    94081 aatggaagcc cgttaactgc agtggatagg ttttccgcag gggatcgtcc cagccatcaa
    94141 aacctggggt atacgcagga aggggatgaa taatttcatc ttttttcaat tcccaggtat
    94201 ctgcaatctt cgccagtcgt tcagaataaa tttcgatttt ccccgaaggt gttttcaacg
    94261 ggtttgcctg tggatcttca cggaatgcgc ggaaagcgac gtagtgttct tccgggcatt
    94321 ttttcttaaa gatcccggtc gttttcatct cctcgtagtc gggcatctca gggttacgtt
    94381 ccttcgtttt cgcatggaga tatttgaccc attcatgctg actgcgacct tcagtaaagg
    94441 tttgataaac gtctggtcct aagcgtttgg cgacttcact cagcatccag tagatgggtt
    94501 tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat cacatagccc atattccctg
    94561 cagattcatg agagataagg tcttcttgct ctgttggcat caggtcgggc aacaggatat
    94621 cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc aacaatcatc tcgcacttgc
    94681 tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc atgttgattg atcaatgtgt
    94741 tactggcgta gcaccataaa aacttgatgg ggacatccag tttttctttt ccacgaacac
    94801 catcacgggt cgcggtcatt tccgtaccat ggtcgatggc atctgtccat gtaaagacgg
    94861 aaatctgcgt tttaacagga ttctcaagca tcgggaacca ttctaccccc agatcccagc
    94921 taccttcgcg tacacctgag ttgccgccgt ttatgccgac gttaccggtg agaacggaaa
    94981 gcatggcaat agcgcgggac gtttgctcgc cgttggaatg tcgttgtggc ccccaaccct
    95041 gacaaatata agcaggtttt gctgaaccga tctctcgtgc caactggata attttttctg
    95101 ccgggatgct ggtgattttt gctgcccatt ccggcgtttt agctatgccg tcaggccctt
    95161 cgcccagaat ataggcttta taatgcgcgt tacgtggtgc gttggcgggc agcgtttttt
    95221 catcgtaacc aacacaatat ttgtcgagaa atggctgatc gaccatgttt tcagtaatca
    95281 gtacccaggc aatcgcacag gccagtgcgc catcggtgcc agggcgaatg ggcagccatt
    95341 catcttcacg cccggcagca gtgtcgttat aacgtggatc gatgacgatc atgcgtgcgt
    95401 ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc accgccgctc atccgcgttt
    95461 ctgccgggtt atttccgaac ataacgacca gtttcgtatt ggcgatatca tccgggctgt
    95521 tgccatcatt ggcaccgaac atataactca ttgcggcact gatctgtgcg gtactgtagc
    95581 tgccatagcg actgagaaaa ccaccgcaag agttcatcag acggtacggg acgtttgagt
    95641 tggtgatgtt tccgccatct acgcctgttc cgtacaggac atgtacagcc tcattgccgt
    95701 aatctttcag gatccgccga agattatcac tgatggtatc cagggcttcg tcccaactta
    95761 tccgttcaaa tttaccttca ccgcgcttgc cgacgcgctt catgggatat ttcaacctat
    95821 caggatgatt catccgtcgg cggatagagc gcccgcgtaa acacgctcga acctgatggt
    95881 taccgtagac gtcgtcacct gtcgtatcag actccaccca gtacacggtg tcatctttca
    95941 catgcaaacg taacagacag cggctcccgc agttaacggt gcaggaactc cagaccgctt
    96001 tctcttctac cggagcctct gccgcccgga ccatttggga aaatggcaga gtgaaagcac
    96061 tgcttgccag cgcaagactg ccaagtgcgg aggttttcat cagacttcta cggctgattt
    96121 cagccttcat gagcgcctct gtggtatgga ttttcatcat tactcactta ttgcttttca
    96181 aacaaaatgt catgccagaa tttatggttg tcgtgggtta tattttttcg atctcgacca
    96241 gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg gcgcagagtg gttagcgtat
    96301 tcacacagcc gccatggtcg attttatcgc cagacatatt ggcctcgtgc caggctccct
    96361 ggcccatagc gctaactcca gggagaatac gtggtgttac tttggctggt agccgaactt
    96421 cgccacgatg gttaaacacc cgcaccatat cgccgttggc aatcccacgt ttctgcgcat
    96481 ctatagggtt gatccacacc tcctgacggc aggcagcctt caggagatca atattgccgt
    96541 aggtcgagtg agtacgggat ttgtaatgga aaccaaacag ttgcagtggg aaggttctac
    96601 gttcagggga gttccagcct tcaaaggttg aggcataaac tggcaatggg cttatcactt
    96661 catctttttc cagttcccag gtacgggcaa tttccgccag cctgctggaa taaatttcaa
    96721 tcttaccgga aggcgtttta agtggatttg cctcggggtc gtcacgaaat gctttgtagg
    96781 cgacaaaatg gccattggga tctttacgct tatagatacc catttttttc agttcgtcgt
    96841 aagacggtaa cgccggatct ttggcaagca ttttggcgta cagatgttgt aaccattgtt
    96901 cctgcgtgcg accttctgtg aacttttgat agacgtcagg tccaagacgt ttcgcgactt
    96961 cactcaggat ccagtaaatc ggtttgcgtt cgaatttttc gctggtgaca ggctggagga
    97021 aaatgagata tcccatgtta ccggcgtagt cgttaggaat aatatcttcc tgctcaacgg
    97081 tcatcaggtc tggcagcaga atgtcggcat attttgccga tgaggtcata aagttttcga
    97141 tgatcacaat catttcgcat ttcgattcgt cctgcagaat ttcatgcgtt ttgttgatgt
    97201 cagaatgctg attaacgagg gtatttcccg cgtagttcca gatgaactta atgggcacat
    97261 ccagtttatc tttgccgcgg acgccgtcgc ggattgccgt catttgcgga ccatgatcga
    97321 tagcatctgt ccagctgaag caggagattg acgttttgac cggattatcc agcaccggca
    97381 ggcgttctat ggtaatggta taggtcgatt cacgcgcgcc actatttccg ccgctgatgc
    97441 cgacattgcc cgtcaaaata ggtaacatag caatagcgcg tgcagtcagt tcgccgtttg
    97501 cctggcgttg cggcccccag ccctggcaga tataagcggg ttttgctgtg ccaatttcac
    97561 gcgccagttt gatgatacgg tctaccggga taccggtaat ttgcgaagcc cactgcggcg
    97621 ttttcgctgt gttatcatca ccttcaccaa gaatataggc tttatagtga ccatttttgg
    97681 gtgcatctgc gggtaaggtt ttttcgtcat agccgacgca gtatttatcg agaaaaggtt
    97741 gatcaacgag attttcgtta atcaataccc aggcaatacc cgcaaccagc gcggcatcgg
    97801 tgcccgggcg aatagggagc cattcatctt cacgaccagc agccgtatcg gtatatcgcg
    97861 gatcgataac aatcattttg gcgttcgatt tctcgcgcgc tttttcaaga agataagtga
    97921 tgccaccgcc gctcatgcgg gtttctgccg ggttgttacc aaacatcacg accagcttgc
    97981 tgttttcaat atccgtggtg ctgttgccat cattactgcc gtaggtgtag ggcatggcac
    98041 aggaaatttg cgcggtgctg taggagccat actgattgag tgaaccgccg tagcagttca
    98101 tcaggcgttt gaccgccgag gctgatggcg aagagcgggt catattgccg ccaacaatcc
    98161 ccgaagagta ctgaatatat acagcctcat tgccatattg ttcgacggtt tttttcaggc
    98221 tactggcgat agtatccagg gcttcatccc agctaatccg ttcgaatttg ccttcaccgc
    98281 gtgtacccac gcgtttcatt gggtaattca agcgatcggg atgattaata cgccggcgga
    98341 tggagcgacc gcgcaaacag gcgcgtacct gatggttgcc gtactcatcg ctgccggtat
    98401 tgtcagtttc cacccaggtc acttcattat ctttaacatg tagacgaagt gcacagcggc
    98461 taccacagtt gacggaacag gcaccccaga tcactttttc gctggcctgt tgtaccgttg
    98521 ccgctgcact gcgcagggtg aacggtaaag aaaaaccgcc tgcagccagc gccagagaac
    98581 ctatcgcggt tgatttaacg agtgttctgc ggctgatgcc caccattcgg tcatttttgg
    98641 acataactca ctccttgttc t
//
LOCUS       DAZUFB010000003        98010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZUFB010000003 DAZUFB010000000
VERSION     DAZUFB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.003"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..221
                     /locus_tag="ACHY3M_000202"
     CDS             <1..221
                     /locus_tag="ACHY3M_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4315197.1"
                     /translation="WISCSERMPKKNQNVLISVNFDSDLVEPLICSARYTGSTFRRGE
                     ATIKPGNGVEQVTHWMPLPEPPQEVNRG"
     gene            577..1215
                     /locus_tag="ACHY3M_000203"
     CDS             577..1215
                     /locus_tag="ACHY3M_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFS4315198.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFVESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMR
                     ILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            1271..1702
                     /locus_tag="ACHY3M_000204"
     CDS             1271..1702
                     /locus_tag="ACHY3M_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFS4315199.1"
                     /translation="MKQIAFYRRSGRPGVFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            1699..2325
                     /locus_tag="ACHY3M_000205"
     CDS             1699..2325
                     /locus_tag="ACHY3M_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4315200.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            2285..2497
                     /locus_tag="ACHY3M_000206"
     CDS             2285..2497
                     /locus_tag="ACHY3M_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFS4315201.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            2533..2922
                     /locus_tag="ACHY3M_000207"
     CDS             2533..2922
                     /locus_tag="ACHY3M_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4315202.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGQ
                     KQKRWEGACKALQELNKYRDLINELSE"
     gene            3066..3299
                     /locus_tag="ACHY3M_000208"
     CDS             3066..3299
                     /locus_tag="ACHY3M_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315203.1"
                     /translation="MKITFESYGLTASVVISSSIFESRKHQHIVDAVKLKAPEVTVTT
                     HGLFRIRTTLTTTHLSACRVYDMALKEYNQCSS"
     gene            3287..3538
                     /locus_tag="ACHY3M_000209"
     CDS             3287..3538
                     /locus_tag="ACHY3M_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4315204.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEQHIASLRKMRRISIN"
     gene            3599..3781
                     /locus_tag="ACHY3M_000210"
     CDS             3599..3781
                     /locus_tag="ACHY3M_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4315205.1"
                     /translation="MEINLMTEKEVSELLQKRRTALYNLRKKHGFPDPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(3765..4934)
                     /locus_tag="ACHY3M_000211"
     CDS             complement(3765..4934)
                     /locus_tag="ACHY3M_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4315206.1"
                     /translation="MALSDTKLRGLYGKNYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPTDGELTLKECLDQ
                     WWDKYVSGLKPNTQTLYRSVVYNTMYTQFEDSPVAGIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKLLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPIFTQ
                     VEPYIERLLNIGFDVLFPGQEIDKPIDRSSANLYMKKLRAKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            5130..5204
                     /locus_tag="ACHY3M_000212"
     tRNA            5130..5204
                     /locus_tag="ACHY3M_000212"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5163..5165,aa:Arg,seq:cct)
     gene            complement(5645..6892)
                     /locus_tag="ACHY3M_000213"
     CDS             complement(5645..6892)
                     /locus_tag="ACHY3M_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFS4315207.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMVLRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(6964..7878)
                     /locus_tag="ACHY3M_000214"
                     /pseudo
     CDS             complement(6964..7878)
                     /locus_tag="ACHY3M_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
     gene            8094..9527
                     /gene="cscA"
                     /locus_tag="ACHY3M_000215"
     CDS             8094..9527
                     /gene="cscA"
                     /locus_tag="ACHY3M_000215"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4315208.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(9535..10530)
                     /locus_tag="ACHY3M_000216"
     CDS             complement(9535..10530)
                     /locus_tag="ACHY3M_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4315209.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            10773..10991
                     /locus_tag="ACHY3M_000217"
                     /pseudo
     CDS             10773..10991
                     /locus_tag="ACHY3M_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            11009..12337
                     /gene="dsdA"
                     /locus_tag="ACHY3M_000218"
                     /pseudo
     CDS             11009..12337
                     /gene="dsdA"
                     /locus_tag="ACHY3M_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(12445..13983)
                     /gene="emrY"
                     /locus_tag="ACHY3M_000219"
     CDS             complement(12445..13983)
                     /gene="emrY"
                     /locus_tag="ACHY3M_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFS4315210.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKLPFTA
                     KGVG"
     gene            complement(13983..15146)
                     /gene="emrK"
                     /locus_tag="ACHY3M_000220"
     CDS             complement(13983..15146)
                     /gene="emrK"
                     /locus_tag="ACHY3M_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFS4315211.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            15562..16176
                     /gene="evgA"
                     /locus_tag="ACHY3M_000221"
     CDS             15562..16176
                     /gene="evgA"
                     /locus_tag="ACHY3M_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFS4315212.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            16181..19774
                     /gene="evgS"
                     /locus_tag="ACHY3M_000222"
     CDS             16181..19774
                     /gene="evgS"
                     /locus_tag="ACHY3M_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFS4315213.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(19830..20975)
                     /gene="yfdE"
                     /locus_tag="ACHY3M_000223"
     CDS             complement(19830..20975)
                     /gene="yfdE"
                     /locus_tag="ACHY3M_000223"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFS4315214.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(21049..21993)
                     /gene="yfdV"
                     /locus_tag="ACHY3M_000224"
     CDS             complement(21049..21993)
                     /gene="yfdV"
                     /locus_tag="ACHY3M_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFS4315215.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(22063..23757)
                     /gene="oxc"
                     /locus_tag="ACHY3M_000225"
     CDS             complement(22063..23757)
                     /gene="oxc"
                     /locus_tag="ACHY3M_000225"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFS4315216.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(23811..25061)
                     /gene="frc"
                     /locus_tag="ACHY3M_000226"
     CDS             complement(23811..25061)
                     /gene="frc"
                     /locus_tag="ACHY3M_000226"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFS4315217.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(25574..26206)
                     /gene="yfdX"
                     /locus_tag="ACHY3M_000227"
     CDS             complement(25574..26206)
                     /gene="yfdX"
                     /locus_tag="ACHY3M_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFS4315218.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            26502..26777
                     /gene="ypdI"
                     /locus_tag="ACHY3M_000228"
     CDS             26502..26777
                     /gene="ypdI"
                     /locus_tag="ACHY3M_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFS4315219.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(26854..27096)
                     /gene="yfdY"
                     /locus_tag="ACHY3M_000229"
     CDS             complement(26854..27096)
                     /gene="yfdY"
                     /locus_tag="ACHY3M_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFS4315220.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            27449..28369
                     /gene="lpxP"
                     /locus_tag="ACHY3M_000230"
     CDS             27449..28369
                     /gene="lpxP"
                     /locus_tag="ACHY3M_000230"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFS4315221.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            28725..28796
                     /gene="ypdK"
                     /locus_tag="ACHY3M_000231"
     CDS             28725..28796
                     /gene="ypdK"
                     /locus_tag="ACHY3M_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFS4315222.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(28861..30099)
                     /gene="alaC"
                     /locus_tag="ACHY3M_000232"
     CDS             complement(28861..30099)
                     /gene="alaC"
                     /locus_tag="ACHY3M_000232"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFS4315223.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            30476..32173
                     /gene="ypdA"
                     /locus_tag="ACHY3M_000233"
     CDS             30476..32173
                     /gene="ypdA"
                     /locus_tag="ACHY3M_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFS4315224.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            32188..32922
                     /gene="ypdB"
                     /locus_tag="ACHY3M_000234"
                     /pseudo
     CDS             32188..32922
                     /gene="ypdB"
                     /locus_tag="ACHY3M_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            32935..33792
                     /gene="ypdC"
                     /locus_tag="ACHY3M_000235"
     CDS             32935..33792
                     /gene="ypdC"
                     /locus_tag="ACHY3M_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4315225.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(33795..36290)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_000236"
     CDS             complement(33795..36290)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_000236"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4315226.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(36315..37352)
                     /gene="ypdE"
                     /locus_tag="ACHY3M_000237"
     CDS             complement(36315..37352)
                     /gene="ypdE"
                     /locus_tag="ACHY3M_000237"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4315227.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(37352..38437)
                     /gene="ypdF"
                     /locus_tag="ACHY3M_000238"
     CDS             complement(37352..38437)
                     /gene="ypdF"
                     /locus_tag="ACHY3M_000238"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4315228.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(38452..39699)
                     /gene="fryC"
                     /locus_tag="ACHY3M_000239"
     CDS             complement(38452..39699)
                     /gene="fryC"
                     /locus_tag="ACHY3M_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4315229.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(39721..40047)
                     /gene="fryB"
                     /locus_tag="ACHY3M_000240"
     CDS             complement(39721..40047)
                     /gene="fryB"
                     /locus_tag="ACHY3M_000240"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4315230.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(40266..41231)
                     /gene="glk"
                     /locus_tag="ACHY3M_000241"
     CDS             complement(40266..41231)
                     /gene="glk"
                     /locus_tag="ACHY3M_000241"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFS4315231.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            41435..42691
                     /gene="yfeO"
                     /locus_tag="ACHY3M_000242"
     CDS             41435..42691
                     /gene="yfeO"
                     /locus_tag="ACHY3M_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFS4315232.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYEQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            42806..43132
                     /gene="ypeC"
                     /locus_tag="ACHY3M_000243"
     CDS             42806..43132
                     /gene="ypeC"
                     /locus_tag="ACHY3M_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFS4315233.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(43273..44511)
                     /gene="mntH"
                     /locus_tag="ACHY3M_000244"
     CDS             complement(43273..44511)
                     /gene="mntH"
                     /locus_tag="ACHY3M_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4315234.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQLLSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            44847..46049
                     /gene="nupC"
                     /locus_tag="ACHY3M_000245"
     CDS             44847..46049
                     /gene="nupC"
                     /locus_tag="ACHY3M_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFS4315235.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(46099..48288)
                     /gene="pdeA"
                     /locus_tag="ACHY3M_000246"
     CDS             complement(46099..48288)
                     /gene="pdeA"
                     /locus_tag="ACHY3M_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS4315236.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(48496..48571)
                     /locus_tag="ACHY3M_000247"
     tRNA            complement(48496..48571)
                     /locus_tag="ACHY3M_000247"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48536..48538),aa:Ala,seq:ggc)
     gene            complement(48611..48686)
                     /locus_tag="ACHY3M_000248"
     tRNA            complement(48611..48686)
                     /locus_tag="ACHY3M_000248"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48651..48653),aa:Ala,seq:ggc)
     gene            48907..49266
                     /gene="yfeC"
                     /locus_tag="ACHY3M_000249"
     CDS             48907..49266
                     /gene="yfeC"
                     /locus_tag="ACHY3M_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS4315237.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            49268..49660
                     /gene="yfeD"
                     /locus_tag="ACHY3M_000250"
     CDS             49268..49660
                     /gene="yfeD"
                     /locus_tag="ACHY3M_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS4315238.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(49712..51127)
                     /gene="gltX"
                     /locus_tag="ACHY3M_000251"
     CDS             complement(49712..51127)
                     /gene="gltX"
                     /locus_tag="ACHY3M_000251"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4315239.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            51386..51461
                     /locus_tag="ACHY3M_000252"
     tRNA            51386..51461
                     /locus_tag="ACHY3M_000252"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51419..51421,aa:Val,seq:tac)
     gene            51466..51541
                     /locus_tag="ACHY3M_000253"
     tRNA            51466..51541
                     /locus_tag="ACHY3M_000253"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51499..51501,aa:Lys,seq:ttt)
     gene            51573..51698
                     /locus_tag="ACHY3M_000254"
     ncRNA           51573..51698
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_000254"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(51807..52691)
                     /gene="xapR"
                     /locus_tag="ACHY3M_000255"
     CDS             complement(51807..52691)
                     /gene="xapR"
                     /locus_tag="ACHY3M_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFS4315240.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(52732..52890)
                     /locus_tag="ACHY3M_000256"
     CDS             complement(52732..52890)
                     /locus_tag="ACHY3M_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFS4315241.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(52943..54199)
                     /gene="xapB"
                     /locus_tag="ACHY3M_000257"
     CDS             complement(52943..54199)
                     /gene="xapB"
                     /locus_tag="ACHY3M_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFS4315242.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(54259..55092)
                     /gene="xapA"
                     /locus_tag="ACHY3M_000258"
     CDS             complement(54259..55092)
                     /gene="xapA"
                     /locus_tag="ACHY3M_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFS4315243.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            55341..56105
                     /gene="yfeN"
                     /locus_tag="ACHY3M_000259"
     CDS             55341..56105
                     /gene="yfeN"
                     /locus_tag="ACHY3M_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFS4315244.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(56144..57070)
                     /gene="yfeR"
                     /locus_tag="ACHY3M_000260"
     CDS             complement(56144..57070)
                     /gene="yfeR"
                     /locus_tag="ACHY3M_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4315245.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            57160..58158
                     /gene="yfeH"
                     /locus_tag="ACHY3M_000261"
     CDS             57160..58158
                     /gene="yfeH"
                     /locus_tag="ACHY3M_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFS4315246.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(58155..58373)
                     /gene="ypeB"
                     /locus_tag="ACHY3M_000262"
     CDS             complement(58155..58373)
                     /gene="ypeB"
                     /locus_tag="ACHY3M_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFS4315247.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(58375..60390)
                     /gene="ligA"
                     /locus_tag="ACHY3M_000263"
     CDS             complement(58375..60390)
                     /gene="ligA"
                     /locus_tag="ACHY3M_000263"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4315248.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(60461..61447)
                     /gene="zipA"
                     /locus_tag="ACHY3M_000264"
     CDS             complement(60461..61447)
                     /gene="zipA"
                     /locus_tag="ACHY3M_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFS4315249.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            61677..62438
                     /gene="cysZ"
                     /locus_tag="ACHY3M_000265"
     CDS             61677..62438
                     /gene="cysZ"
                     /locus_tag="ACHY3M_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFS4315250.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            62623..63594
                     /gene="cysK"
                     /locus_tag="ACHY3M_000266"
     CDS             62623..63594
                     /gene="cysK"
                     /locus_tag="ACHY3M_000266"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4315251.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            63978..64235
                     /gene="ptsH"
                     /locus_tag="ACHY3M_000267"
     CDS             63978..64235
                     /gene="ptsH"
                     /locus_tag="ACHY3M_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFS4315252.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            64280..66007
                     /gene="ptsI"
                     /locus_tag="ACHY3M_000268"
     CDS             64280..66007
                     /gene="ptsI"
                     /locus_tag="ACHY3M_000268"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFS4315253.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            66048..66557
                     /gene="crr"
                     /locus_tag="ACHY3M_000269"
     CDS             66048..66557
                     /gene="crr"
                     /locus_tag="ACHY3M_000269"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4315254.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(66599..67450)
                     /gene="pdxK"
                     /locus_tag="ACHY3M_000270"
                     /gene_synonym="thiJ"
     CDS             complement(66599..67450)
                     /gene="pdxK"
                     /locus_tag="ACHY3M_000270"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFS4315255.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            67555..67923
                     /gene="yfeK"
                     /locus_tag="ACHY3M_000271"
     CDS             67555..67923
                     /gene="yfeK"
                     /locus_tag="ACHY3M_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFS4315256.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(67926..68837)
                     /gene="cysM"
                     /locus_tag="ACHY3M_000272"
     CDS             complement(67926..68837)
                     /gene="cysM"
                     /locus_tag="ACHY3M_000272"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFS4315257.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68971..70068)
                     /gene="cysA"
                     /locus_tag="ACHY3M_000273"
     CDS             complement(68971..70068)
                     /gene="cysA"
                     /locus_tag="ACHY3M_000273"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFS4315258.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIKTRDEELALAQSA"
     gene            complement(70058..70933)
                     /gene="cysW"
                     /locus_tag="ACHY3M_000274"
     CDS             complement(70058..70933)
                     /gene="cysW"
                     /locus_tag="ACHY3M_000274"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFS4315259.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            complement(70933..71766)
                     /gene="cysT"
                     /locus_tag="ACHY3M_000275"
     CDS             complement(70933..71766)
                     /gene="cysT"
                     /locus_tag="ACHY3M_000275"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFS4315260.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(71766..72782)
                     /gene="cysP"
                     /locus_tag="ACHY3M_000276"
     CDS             complement(71766..72782)
                     /gene="cysP"
                     /locus_tag="ACHY3M_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFS4315261.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(72953..73744)
                     /gene="ucpA"
                     /locus_tag="ACHY3M_000277"
     CDS             complement(72953..73744)
                     /gene="ucpA"
                     /locus_tag="ACHY3M_000277"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFS4315262.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(73873..74730)
                     /gene="murR"
                     /locus_tag="ACHY3M_000278"
     CDS             complement(73873..74730)
                     /gene="murR"
                     /locus_tag="ACHY3M_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFS4315263.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            74894..75790
                     /gene="murQ"
                     /locus_tag="ACHY3M_000279"
     CDS             74894..75790
                     /gene="murQ"
                     /locus_tag="ACHY3M_000279"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4315264.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKLGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            75794..77218
                     /gene="murP"
                     /locus_tag="ACHY3M_000280"
     CDS             75794..77218
                     /gene="murP"
                     /locus_tag="ACHY3M_000280"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFS4315265.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            77223..78527
                     /gene="pbp4b"
                     /locus_tag="ACHY3M_000281"
     CDS             77223..78527
                     /gene="pbp4b"
                     /locus_tag="ACHY3M_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFS4315266.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYDHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(78585..79484)
                     /gene="yfeX"
                     /locus_tag="ACHY3M_000282"
     CDS             complement(78585..79484)
                     /gene="yfeX"
                     /locus_tag="ACHY3M_000282"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFS4315267.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGIHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(79580..80137)
                     /gene="yfeY"
                     /locus_tag="ACHY3M_000283"
     CDS             complement(79580..80137)
                     /gene="yfeY"
                     /locus_tag="ACHY3M_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFS4315268.1"
                     /translation="MLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQGVGELT
                     ASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQGTISRID
                     VLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKVEGSQHISYQFSGEW
                     SGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(80217..80666)
                     /gene="yfeZ"
                     /locus_tag="ACHY3M_000284"
     CDS             complement(80217..80666)
                     /gene="yfeZ"
                     /locus_tag="ACHY3M_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFS4315269.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(80653..81078)
                     /gene="ypeA"
                     /locus_tag="ACHY3M_000285"
     CDS             complement(80653..81078)
                     /gene="ypeA"
                     /locus_tag="ACHY3M_000285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFS4315270.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNSEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            81292..82161
                     /gene="amiA"
                     /locus_tag="ACHY3M_000286"
     CDS             81292..82161
                     /gene="amiA"
                     /locus_tag="ACHY3M_000286"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFS4315271.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            82165..83064
                     /gene="hemF"
                     /locus_tag="ACHY3M_000287"
     CDS             82165..83064
                     /gene="hemF"
                     /locus_tag="ACHY3M_000287"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFS4315272.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(83070..84122)
                     /gene="eutR"
                     /locus_tag="ACHY3M_000288"
     CDS             complement(83070..84122)
                     /gene="eutR"
                     /locus_tag="ACHY3M_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFS4315273.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(84168..84668)
                     /gene="eutK"
                     /locus_tag="ACHY3M_000289"
     CDS             complement(84168..84668)
                     /gene="eutK"
                     /locus_tag="ACHY3M_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFS4315274.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(84681..85340)
                     /gene="eutL"
                     /locus_tag="ACHY3M_000290"
     CDS             complement(84681..85340)
                     /gene="eutL"
                     /locus_tag="ACHY3M_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFS4315275.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(85350..86237)
                     /gene="eutC"
                     /locus_tag="ACHY3M_000291"
     CDS             complement(85350..86237)
                     /gene="eutC"
                     /locus_tag="ACHY3M_000291"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFS4315276.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(86258..87619)
                     /gene="eutB"
                     /locus_tag="ACHY3M_000292"
     CDS             complement(86258..87619)
                     /gene="eutB"
                     /locus_tag="ACHY3M_000292"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFS4315277.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RITGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(87631..89034)
                     /gene="eutA"
                     /locus_tag="ACHY3M_000293"
     CDS             complement(87631..89034)
                     /gene="eutA"
                     /locus_tag="ACHY3M_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFS4315278.1"
                     /translation="MNTRQLLRVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(89031..90257)
                     /gene="eutH"
                     /locus_tag="ACHY3M_000294"
     CDS             complement(89031..90257)
                     /gene="eutH"
                     /locus_tag="ACHY3M_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFS4315279.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(90374..91561)
                     /gene="eutG"
                     /locus_tag="ACHY3M_000295"
     CDS             complement(90374..91561)
                     /gene="eutG"
                     /locus_tag="ACHY3M_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFS4315280.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(91551..92387)
                     /gene="eutJ"
                     /locus_tag="ACHY3M_000296"
     CDS             complement(91551..92387)
                     /gene="eutJ"
                     /locus_tag="ACHY3M_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFS4315281.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(92398..93801)
                     /gene="eutE"
                     /locus_tag="ACHY3M_000297"
     CDS             complement(92398..93801)
                     /gene="eutE"
                     /locus_tag="ACHY3M_000297"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFS4315282.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(93813..94100)
                     /gene="eutN"
                     /locus_tag="ACHY3M_000298"
     CDS             complement(93813..94100)
                     /gene="eutN"
                     /locus_tag="ACHY3M_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFS4315283.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(94207..94500)
                     /gene="eutM"
                     /locus_tag="ACHY3M_000299"
     CDS             complement(94207..94500)
                     /gene="eutM"
                     /locus_tag="ACHY3M_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFS4315284.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(94539..95555)
                     /gene="pta"
                     /locus_tag="ACHY3M_000300"
     CDS             complement(94539..95555)
                     /gene="pta"
                     /locus_tag="ACHY3M_000300"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4315285.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(95552..96355)
                     /gene="eutT"
                     /locus_tag="ACHY3M_000301"
     CDS             complement(95552..96355)
                     /gene="eutT"
                     /locus_tag="ACHY3M_000301"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFS4315286.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(96352..97053)
                     /gene="eutQ"
                     /locus_tag="ACHY3M_000302"
     CDS             complement(96352..97053)
                     /gene="eutQ"
                     /locus_tag="ACHY3M_000302"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFS4315287.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(97028..97507)
                     /gene="eutP"
                     /locus_tag="ACHY3M_000303"
     CDS             complement(97028..97507)
                     /gene="eutP"
                     /locus_tag="ACHY3M_000303"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFS4315288.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(97520..97855)
                     /gene="eutS"
                     /locus_tag="ACHY3M_000304"
     CDS             complement(97520..97855)
                     /gene="eutS"
                     /locus_tag="ACHY3M_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFS4315289.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        24831 a        25769 c        23653 g        23757 t
ORIGIN      
        1 gttggataag ctgtagtgag cgaatgccga aaaagaatca gaacgtactt atttcggtga
       61 atttcgatag tgatctggtt gagccgctaa tatgctccgc acgctatacc ggaagcacct
      121 ttcggcgagg agaagcaacg attaagccgg gcaatggtgt tgagcaggta acacactgga
      181 tgccgctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      241 ggtgaatact tcgcagatag tttcccccaa atctggggaa aagcccgaat ggcgcggctt
      301 acagcaagat aaggcctaca tgatttgaca aatccgccag agctatcgca tactgaccgc
      361 actagaactt caaagcggtc atccgcaccc gatagctttg cggctttttt atgcctgcaa
      421 tttggcatag tcacatccgt acaaaggtcg ggtggagagg cgtaatacaa tacccgcaag
      481 gggaatatgc ccggagcatc tttgaaggct ctagttgaca cccgatcacc agccactaac
      541 tggtgattgc taactaaaac ttcaaaggag gtcatcatga ccagtcaact catccccgta
      601 ttcaacggca ctatcgacaa cgaaacaatt cttctagtta acgctcgtga tctacactct
      661 tttcttggtg tgggcagaat gttcgcgcac tgggttaaag aacgcattgc tgaatatgga
      721 ttcgttgaaa gccaggacta tattttgatt tgccaaaatg ggcaaaccaa agggcgagga
      781 ggtgatcgcc gtagcaagga ctaccaccta actctcgaca cagccaaaga actggcgatg
      841 gttgagcgta acgaaaaagg ccgccagatc cgccgatact tcatcgagtg cgaaaagaaa
      901 cttcgccaga gccttttacc tgcaccaatg aacatcaact accctctatc gtggttttca
      961 gagcaccacc cctactccat gatgagctat gttgatcgaa aaactcttaa cctggacgtt
     1021 tctgtgctct tcgatatgcc aagcccaact atgcgcatcc tcaacgagct acacagcaag
     1081 ggctataacg ttgacgccgc tgtcgccgaa tttaacgcct tcaaacatct gacggaagaa
     1141 atgcgccgta ctctacagga tatttcaaga ttgtcagatc gaaattcccg aaaaggcttc
     1201 tcgttaagcc tgtgacttcc ccgaccatcc ccgacatccc gtcggggttt tcatatctgg
     1261 agacaaaaat ttgaaacaga tcgctttcta caggcgttct ggaaggcccg gtgtcttccg
     1321 tgggttaaaa gaacgcgtca cctggatgat tcagtctcgt ggtcgtcctg ttactggcag
     1381 tgaaatagca gagaaatttg gtgtttcact ttgcgagttc aacaaggtag ctcgcggcct
     1441 gacaaagggc agtaaggttg tgaaaataaa ggcttcagaa ccattcacca cagacaccgg
     1501 aatcgttgat cgctttttct ccctcgaatc aaatcctcgt cgtgacacac ctcgctcacg
     1561 caatgccgtt cctccattca gtcgcagaag ccgtgaacac gcagcaaaaa actgtcgcga
     1621 ggaatacgtg caaaaggccg aacgccgtcg ccgactgatt aaagcaggac tttacattga
     1681 tgagtttgaa aacgcgctat gacgaaaaaa tacaccctca tttacgcaga tcccccctgg
     1741 acattccgcg acaaagcaac cgatggtcaa cgcggtgcaa gttttaaata tccggtcatg
     1801 agtcttctgg atatctgccg cctcccggta tgggaactgg cagccgataa ttgcctgttg
     1861 gctatgtggt gggtgcctac acaaccactt gaagcattga aggttgtaga agcgtggggc
     1921 tttcgtctgg tgacgatgaa aggattaacc tggaacaaat gcgggaaaag acagaccgac
     1981 aagctggtca tgggtatggg tagcaccact cgcgctaaca gcgaagactg cctttttgca
     2041 gtgaaaggaa atctgcccga acgcattaac gccggaataa tccagtcatt cactgcacca
     2101 cgccttgatc actcccgcaa gccggatatg gctcgagaaa aacttgtgca acttcttggc
     2161 gatgttcccc ggatagaact gttcgcccgc cacacctcgc atggatttga tgtatggggt
     2221 aaccaatgcg gcacaccatc cattgagatg gttccgggta ttgttaaatt tctggagaaa
     2281 accaatgagc gaaaaaacga cgttgacaaa ggcatcacca gttgaattaa ggcagtgtct
     2341 ggaaatcgca aatcaacttg ccagaagtgg aatacgattt gttccaatcc cgattacagc
     2401 agatgcagaa cttcatctgt ttggtgaaat tctttcccga aagctggatg aactggaaaa
     2461 gctggtagaa gaagctgaca cctcaccaac cgtataacag ccccaccgac attaaaatat
     2521 caggagaaaa aaatgaacgc agtgctcaca gaattgaaca aattaggaaa agcatcagcc
     2581 gaaagtattt ctaaaggtct caatattgat ttgaatgacg ttattgacac tctatggaag
     2641 ttaaaaaacc agggggtagt aactgtaaaa aatggcatct ggcaggcagt tgcaagggaa
     2701 gtggacaaaa aaccaaatat cgcctcagtg cagccagtgc agccagtgca gccagtgcag
     2761 ccagtgcagc cagtgcagca caacattata ggtgacctgc tacgtaaatc acggaaagaa
     2821 gcgcgtcgcg ccgggcagaa acagaaacga tgggagggtg catgtaaggc gctgcaagaa
     2881 ctgaataaat accgtgactt gatcaacgaa ttgtcagagt gagcgaatca cagagagaat
     2941 attttgagaa acaacccgcg tacattaata ttgtttttcc tttctctaat gacgggcggt
     3001 atcctgttat tcgcaggaac aacattattt atttttgttg cccggctaac tgcgaggata
     3061 atgacatgaa aatcacattt gaaagttacg gtttaacagc aagtgtggtt atctccagct
     3121 ctatattcga aagccgcaaa catcagcata tcgttgatgc agtgaaactc aaagccccgg
     3181 aagttaccgt cacaacacat ggacttttca ggatacgaac gacgttgact acaacacacc
     3241 tctcggcatg ccgtgtttat gatatggcac tcaaggaata caaccagtgt tcgtcctgat
     3301 ccagcgcggg cagtctttcg ttgatgccaa caactatccg gtagaaatat gcaaggtaac
     3361 tctgactcag gtgatctacc gaaggctcga cggcagaacc agagccactt caattggtgc
     3421 atttaatgaa gaatttgagc gaatcgagca caacgaacta cacatgatta aagcggaaat
     3481 tgagaaggaa cagcatattg ccagccttcg caaaatgcgc cgcatatcaa tcaactaaca
     3541 accgccttcg ggcggtttat tatgctgttg aaaaacatgt agaaaaggag agcatactat
     3601 ggaaatcaac ttaatgaccg aaaaagaagt ttctgagttg ttacaaaaaa ggcgcacagc
     3661 tttgtataat ttgcgaaaaa aacacggatt ccctgatcca gtacttactc atccggcacg
     3721 atatagtcgt caggccgttg aacaatggct taaagccgga ggaattaacc gagctgttta
     3781 gcgtgccaga aaattttatc agcatacaac tcatacgcat ctttctgttc cgacaaccag
     3841 tcgtgtttat tatacacggc catcactcct cccagttcat gccccagcat tttttcagtg
     3901 acgtggggca taattccttc cccagataaa ttcgttacca gagaacgcct aaaatcatgc
     3961 gttctccatt caggaatatc aattttcgcc ctgagttttt tcatgtacaa attagcagac
     4021 gaacggtcaa taggtttatc tatttcctgc ccagggaaca gcacatcaaa tccgatatta
     4081 agcaatcgct caatataagg ctcaacctga gtaaatatcg gacgccggat aatattaccc
     4141 atctttgaat gctctttagg ggttgtccag accagatctt ccatgttgaa ctcactggct
     4201 gtagcgagac gtagctcaga aagcctggcc ccccatagca atagcaattg atgtaacagc
     4261 ttgttggaag taaccacttt tgagttctcc agtgccagcc agatctttgc cagttccgta
     4321 taggtaagta cgcgacttcc cacatctggt ttcttcccaa tattctttac actgagtttg
     4381 agtaattcgc atgatgggat taactggcgg ctgatacacc agttaataac tgaacgcagt
     4441 tgtaacaaca gaacacgagc ctttttcttg ttgaggcttt cttgcttgtc aaaaaagcga
     4501 gcccaggccg atacagggat accggcaact ggcgagtctt caaactgtgt gtacatagtg
     4561 ttgtacacaa cagatctata aagcgtctga gtattgggtt tcaggccgct aacatactta
     4621 tcccaccact gatccaggca ttcttttaat gtcaattcgc catcagtggg ggcaaaataa
     4681 tttttagggt taactccctt catgtacaat gcgcgcatct caccgacgat aacacgtgca
     4741 tccttcagtg aagtggacgg atagcgcccg acagtaagac gtacgggctt accattccag
     4801 cgataacggt actgaaaggt gatagttcct gctggtgtaa tccgcacact taacccgtcg
     4861 ccatcggtga tttcagcagg gccagagtag ttttttccgt agaggcctcg tagtttggtg
     4921 tcgctcagtg ccaatgcttt atatcctgta cacaagttta taatgtattc tgtactcaat
     4981 atgtacgcat tagcaagtga acaaaacgtt ttttaacgca aaagaagaca atcaatcatg
     5041 aacaaaaacg cacttacacc ttgattttaa aaccataagg acggtattat cagtcatatg
     5101 gtgaacaacc aaaatcaaca cgcaacaatg tcctcttagt taaatggata taacgagccc
     5161 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accagatacc cttcaaacga
     5221 aatctacctt caccccgtaa aagataggtt tggcagcaca cttgccttat atctactcat
     5281 ttttactgca acaggttgaa atctcagcac tgtcagaaag cgctgatgac taaacagccc
     5341 tgggccgggc gatgtaacca tcacacagaa tcctgatagc gaaatatggc gtgactcgat
     5401 actttactcc gcaatgcatt ccttgatgaa ttcgcaggac cgtgatacac gggacaggtc
     5461 gctgaatgac gacaatgtcc tggaaatcag cgaaccgcgc atctgaagta catttgagcg
     5521 actgtaccag aacatgaatg aggcgtttgg attaggtgat tattagcagg gctaagcact
     5581 ttagtattat tattttccgg ttgagggata gggagatatc gacaacaacc ggaaaagttt
     5641 acgtctatat tgctgaaggt acaggcgttt ccataactat ttgctcgcgt tttttactca
     5701 ggaagaaaat gccaaatagc aacatcaggc agacaatacc cgaaattgcg aagaaaactg
     5761 tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt tgaaagcagc acaatcccaa
     5821 gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga cgacaggcgc ttatcaaaat
     5881 ttgccacgct gtatttgaag acggatatga cacaaagtgg aacctcaata gcatgtaaca
     5941 gcttcactaa tgaaataatc caggggttaa cgaacagcgc gcaggaaagg atacgcaaca
     6001 ccataatcac aacaccgata agtaatgcat tttttggccc tacccgattc acaaagaaag
     6061 gaataatcgc catgcatagc gcttcgagta ccacctggaa tgagttgaga taaccataca
     6121 ggcgcgttcc tacatcgtgt gattcgaata aacctgcata aaagacagga aaaagttgtt
     6181 gatcaaaaat gttatagaaa gaccacgtcc ccacaataaa tatgacgaaa acccagaagt
     6241 ttcgatcctt gaaaactgcg ataaaatcct ctttttttac ccctcccgca tccgccgcta
     6301 cgcactggtg atccttatct ttaaaacaca tgttgatcat cataaataca gcgccaaata
     6361 gcgagaccaa ccagaagttg atatggggac tgatactaaa aaatatgccg gcaaagaacg
     6421 cgccaatagc atagccaaaa gatccccagg cgcgcgctgt tccatattcg aaatgaaaat
     6481 ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca tcccgccaga taccccaggc
     6541 caaaaaagag cgcccccaga attagaccta cagaaaaatt gctttgcagt aacggttcat
     6601 aaacgtaaat cataaacggt ccggtcaaga ccaggatgaa actcatacac cagatgagcg
     6661 gtttcttcag accgagttta tcctgaacga tgccgtagaa catcataaat agaatgctgg
     6721 taaactggtt gaccgaataa agtgtaccta attccgtccc tgtcaaccct agatgtcctt
     6781 tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag agaaatgagt
     6841 aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
     6901 acctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc cgtttacttc
     6961 tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg ccgttactgc
     7021 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct catctgtaga
     7081 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
     7141 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
     7201 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
     7261 ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
     7321 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
     7381 gtcttaccat agatcttcac gaatattagg atcgaagctg acaaaacctc cggcatgccg
     7441 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
     7501 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
     7561 atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt tcagatcgac
     7621 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgactccctc
     7681 agttagcagc gttccttgca ttaacgcccc aaaaggatca tcaccgaccc gacctataaa
     7741 tccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
     7801 acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
     7861 ccatactttg gctgacattt ttttccctta aattcatctg agttacgcat agtgataaac
     7921 ctctttctcg caaaatcgtc atggatttac taaaacatgc atattcgatc acaaaacgtc
     7981 atagttaacg ttaacatttg tgatattcat cgcatttatg aaagtaaggg actatatttt
     8041 tataaaagtt aacgttaaca attcaccaaa tttgcttaac caggatgatt aaaatgacgc
     8101 aatctcgatt gcatgcggcg caaaacgcac tagcaaaact tcatgagcac cggggtaaca
     8161 ctttctatcc ccattttcac ctcgcgccac ctgccggatg gatgaacgat ccaaacggcc
     8221 tgatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccgatg agcgaacact
     8281 ggggaccaat gcactgggga catgccacca gcgacgatat gatccactgg cagcatgagc
     8341 ctattgcgct agcgccagga gacgataatg acaaagacgg gtgtttttca ggtagtgctg
     8401 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
     8461 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
     8521 tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc cgcgatccta
     8581 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca
     8641 cggggcagat cctgctttat cgcggcagtt cgttgcgtga atggaccttc gatcgcgtac
     8701 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
     8761 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
     8821 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
     8881 aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttt
     8941 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
     9001 cctcaaaacg tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
     9061 atggcaaact tctacaacgc ccggtacacg aagctgagtc gttacgccag cagcatcaat
     9121 ctgtctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
     9181 agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg
     9241 gcactggaat gcggctgtat attgataacc aatctgagcg acttgttttg tggcggtatt
     9301 acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg
     9361 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg
     9421 tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc
     9481 acggagtggc tgtgctgcaa catggagcac tctggctact gggttaacat aatatcaggt
     9541 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgaccttc
     9601 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
     9661 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cggcgacatc
     9721 ctgcgggatt cgtaccccct tcgccagaag aacctgataa gccacaaagg ctgcgcgatc
     9781 gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtggt cattgagtaa
     9841 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
     9901 cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat accctgttgc
     9961 caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg cgagcaaatg
    10021 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
    10081 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgtgat
    10141 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
    10201 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccgaa
    10261 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtagccgt
    10321 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
    10381 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
    10441 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
    10501 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
    10561 gtagcagaaa cagacgtcac tggcgagatc caacgatatt ctcaacgccc tatcacccga
    10621 cacggcaata caataaaaaa taacaataat tgccggataa ttgtactcaa ttccgcctct
    10681 gttctcgcca acgagtaacc atataggcaa gtaagtaaaa catgccaact ggcatcgcca
    10741 acctggagaa gaaaatataa atatggatca ttgtgctgcc gctgtatccc gacatcagcc
    10801 cggaaattat tgcgattgct atcggttcag gtgcaattgg ctgcacgatc gttacggact
    10861 cgcttttctg gctagtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
    10921 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
    10981 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
    11041 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg
    11101 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
    11161 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc
    11221 tgccactggg gggattattg aatcagaact ggttgccatt ccagctatgc aaaaacggct
    11281 ggaaaaagaa tatcagcaac cgatcagcgg gcaactgtta ctgaaaaaag atagccattt
    11341 gcccatttcc ggctccataa aagcacgcgg cgggatttat gaagtcctgg cacacgcaga
    11401 aaaactggct ctggaagcgg ggttgctgac gcttgatgat gactacagca aactgctttc
    11461 tccggagttt aaacagttct ttagccaata cagcattgct gtgggctcaa ccggaaatct
    11521 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag ttcatatgtc
    11581 tgctgatgcc cgggcatagc aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
    11641 atatgagcaa gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc
    11701 gaactgtttc tttattgatg acgaaaattc ccgcacgttg ttccttgggt attccgtcgc
    11761 tggccagcgt cttaaagcgc aatttgccca gcaaggtcgt atcgtcgatg ctgataaccc
    11821 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcggact
    11881 taaactggcg tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
    11941 tatgttgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
    12001 cgacaacctt accgcagcgg atggccttgc agttggtcgc gcatcaggct ttgtcgggcg
    12061 ggcaatggag cgtctgctgg atggcttcta tacccttagc gatcaaacca tgtatgacat
    12121 gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa ccttcagcac tggcgggtat
    12181 ggccggatct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
    12241 agaacaattg cgtaatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
    12301 agaagagatg aatcaatatc tggcaaaagg ccgttaataa cgtttcaacg cagcatcgca
    12361 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cgctcttacc
    12421 cgctataacc ccctcctttt atcatcaccc aacgcctttc gctgtaaacg gcagtttcgc
    12481 aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
    12541 tgcagaaata gaaagtgact gctgggttat ttcattatta atttcattaa gaactcctga
    12601 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaacacgg ggttaaattg
    12661 atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
    12721 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
    12781 attggcaaat ttattatctg gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa
    12841 acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt
    12901 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactaaatgt
    12961 cactaacaac cgcatgtcta ttttgttgcc ataacgtcct atcaaaggtg aaattaatag
    13021 tggcatgatg ccgatgggcg cataagcaag tccggcccat atcgcattat accccatcgt
    13081 ttcctggagt aactgcggca taaggacgat cgctccagag taaaataaat acgcgcatgt
    13141 gatactcaca ataccaatgg tgaagttacg ggacttaaac aaactgagat caagaatcgg
    13201 gttctctgag gtcgactccc aaatgactaa agagatcaga gaaataactg atactactgt
    13261 taatattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcataat
    13321 ttgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca ttttgaccgg
    13381 tgaagtttca gtttctcttc ctttaagtaa ggttaagcat aatgtcagga cgataatccc
    13441 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tataaccgcc
    13501 caatatcggc ccacatatcg gagcgataat cacggtcatt gaccataatg ccagagcaaa
    13561 tgttcttttt tctggtggat aattccttaa taataaactc tgtgacagtg gaattaacgg
    13621 ccccgccatt aatccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgataa
    13681 actacacatt aaggaagaca gcgaaaaaaa agtgactgaa agtaaaaata accttaattc
    13741 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
    13801 cgaggtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
    13861 tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta atgacaatgc
    13921 aatagtaacg caccataacg tcccaccggt taatggtgcc ggagtcgatt tagtgattgc
    13981 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggactggta
    14041 tcgataacta aagccttact ggtataagcc ggcatggacg tcacggttga agccagatca
    14101 ggcatctcag caatgtcttc gttcttcgta tcaatagttg cagtcatcga taaaccaatg
    14161 cgtaaggggt gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
    14221 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
    14281 gttcccatat tgatccctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg
    14341 ctgataatat tgactgattg accaatccgt acatccgtga gttgtgtttc tttaaagttg
    14401 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc gggactcact
    14461 gtttcgccga cctgaacact ccgctgggca atatagccag taaccggact ccttatatcc
    14521 gtacgtttaa gcgccaacca ggcttcttta gttgcatccg ccgcttccac gacttgtggc
    14581 tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagcg
    14641 gcattcagtg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
    14701 gaaataaccc cctgcttcgc taacggtact cgacggttat aatcttctaa agattgttga
    14761 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtacagttta
    14821 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagagc gatagtggca
    14881 tcagttttat ccagtgaaac taaaatgtca ccttgtcgaa cgtagttcgt atctttatga
    14941 ttaacgacag tgacactacc tgagacttgt gcagaaattg gatctgcatt ccccgtgaca
    15001 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataggcacct
    15061 gaaaacgcaa taaataaaac taccgccaat aaagaaaagt attttcttct gttagaatgt
    15121 tttttatttg aattaatctg ttccactatt atctctcatt tctcatagat gaaatttatg
    15181 aattgaatga aaagagcatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
    15241 cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
    15301 cgtggctttt gcaatacaat tcttacgcct gtaggattag tgagaagact tatagtgcta
    15361 acttgaaact ataaatcatc ggtataatcc ctgattttat tgttgacatt tcatttatgc
    15421 cgactattta tatggtatac ttgtcgaatt atcttaaagg aagctcagat tttcttattt
    15481 ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa gggagatgct
    15541 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
    15601 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttgactgaa
    15661 gggggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc
    15721 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
    15781 ggaattatta ttattgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
    15841 gctggcgcta atggattcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt
    15901 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
    15961 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
    16021 tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa
    16081 actgtcagca cttataaaag tcgcctgatg gaaaaattag aatgtaaatc actgatggat
    16141 ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt taccctattt
    16201 ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag attacatcga
    16261 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc tgagcaacaa
    16321 ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
    16381 ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg ctgattattt
    16441 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg ccgatcatca
    16501 aaaagcaatg gacgcgcttg cagaaggtga agtcgatata gtgttatcac atttagttac
    16561 ttcgccgcct cttaatagtg acattgctgc aaccaaacca ttgataatta cctttccggc
    16621 gctggtaacc acccttcacg actcaatgcg accgcttacc tcaccaaaac cagtaaatat
    16681 tgctcgggta gcaaattacc ccccagacga ggtaattcat caatcgtttc caaaagcaac
    16741 aattatctct tttacaaatt tatatcaggc attagcatcc gtatcagccg gacagaatga
    16801 ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt tcactcactc
    16861 cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat aattttttct tgaccagaaa
    16921 agaatccgtc attcttaatg aagtactcaa tagatttgtt gatgctttaa caaatgaagt
    16981 tcgctatgaa gtttcacaaa attggcttga tacaggaaac ctggcctttc tgaacaaacc
    17041 attagaactc actgaacatg aaaaacagtg gattaagcaa catcccgatt taaaggtgct
    17101 ggaaaatcct tactctcctc cctattctat gacggatgaa aatggctcgg ttcggggcgt
    17161 tatgggggat attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac
    17221 cgtttcacac aatatccatg ccggaacaca gcttaacccc ggtggatggg atataatacc
    17281 tggcgctatt tatagtgaag atcgagaaaa taatgtttca tttgcagaag ctttcataac
    17341 aacgccatac gtttttgtca tgcaaaaagc acctgacagt gaacaaacat taaaaaaagg
    17401 aatgaaagtt gccattccat attattatga gctgcattcg caattaaaag agatgtatcc
    17461 ggaggttgaa tggatacagg tcgataatgc cagcgctgca tttcacaagg ttaaggaagg
    17521 tgaacttgat gctctggtcg cgacacagtt aaattcgcgt tacatgatcg atcattacta
    17581 tcctaatgaa ctttatcatt ttcttattcc tggcgttccg aatgcatcgc tttcgttcgc
    17641 ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga atgcaattcc
    17701 cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa atgcccaatg tgaccattga
    17761 cacatgggac ctatatagcg agcaatttta tattgttacg acattatccg ttttattagt
    17821 tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagtcat
    17881 tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca ctctcggatt ccttaccgaa
    17941 tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cataatagtg cttttgaaca
    18001 ttatttcact gcggattact acaaaaatgc aatgttacca ttagaaaaca gtgactcacc
    18061 ctttaaagat gttttttcta atgcgcatga agtcacagca gaaacgaaag aaaatcgaac
    18121 aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg
    18181 gcatacatta tgcaatcttc ctgcaagtga caatgcagta tatatttgtg gttggcaaga
    18241 tattactgaa acgcgtgatc taattaatgc actcgaggta gaaaaaaata aagcgataaa
    18301 ggctaccgta gcaaaaagtc agtttctggc aacgatgagt cacgaaataa gaacaccaat
    18361 aagctctatt atgggcttcc tggaacttct gtcgggttct ggtcttagca aggagcaacg
    18421 ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct taattggtga
    18481 aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga
    18541 tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc
    18601 gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga tcgaccctca
    18661 ggcgtttaag caggtcttat caaatttact gagcaatgct ctcaaattta ccaccgaggg
    18721 ggcagtaaaa attacgacct ccctggttca cattgatgac aaccacgctg ttatcaaaat
    18781 gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac tctttaaacg
    18841 ctacagccaa acaagtgcag gtcgccagca aacaggatct ggcttaggct taatgatctg
    18901 caaagaatta attaaaaata tgcagggcga tttgtcatta gaaagtcatc caggcatagg
    18961 aacaacattt acgatcacaa tcccggtaga aattagccag caagtggcga ctgtcgaggc
    19021 aaaagcagaa caccccatca cactacctga aaagttgagc atattaatcg cggatgatca
    19081 tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga
    19141 agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg atctgcttat
    19201 tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac tccgtgagca
    19261 aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa
    19321 agggttaagt tgcggcatga acttatgttt gttcaaaccg ttgaccctgg atgtactgaa
    19381 aacacattta agtcagttac accaagttgc gcatattgca cctcagtatc gccaccttga
    19441 tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg agattctcat
    19501 gactttccag catgaaacgc ataaagatct acccgctgcg tttcaagcac tagaagctgg
    19561 cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa
    19621 tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgttt cagatgacag
    19681 taagcctgaa attcttcagt tgctgaactc tgtaaaagaa cacattgcag agctggacca
    19741 ggagattgct gttttctgtc agaaaaatga ctaaatagcg gctcccacaa tgttcaaatg
    19801 tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg aatttgttcc
    19861 ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca gccgctgatt
    19921 tttatcggat tacccggcat cattattccc ccggcttcaa tcaacatatt tctcgcctga
    19981 gtttgtggca atttaatggc ctcagccaca cttaataacg gcgcgacggg tacaccaact
    20041 tcatgtattc tggctaacca aacttcagct gcctgcgttt ttaacgtccg ctcaatatat
    20101 tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg gggatcatta
    20161 accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc attaccacaa
    20221 caaatcgtaa tcggcttatc ctgagtattg aaaacatcaa aaggtgccat gtagggatgg
    20281 cgatttccca gacgttgtgg tgacttccct gtcgcgatat atgccatcag accatgctcc
    20341 agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct ctggctcttt
    20401 tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc gcatagatcc
    20461 gcaagagatg taccaacgcg cactggcgga gcatcaggat atcctgtttc catcattatc
    20521 ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt
    20581 ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgaag cgtttcccat
    20641 gaaaacccca gtttttccat tgtacctggg cgaaaattct cagctaatac atcagcttgt
    20701 ttgagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc
    20761 tctttgccat gattaataaa actgtaatag agtgactgtc catccacata gggaccaaat
    20821 gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc gcccatatta
    20881 caaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat aactaataag
    20941 ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga aaaaagccga
    21001 aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt atgaaaccag
    21061 tcgactgaca taaatccaca agggagccgt gacgacaaag cccagtacgc tcacagccaa
    21121 tgacgctgta ccagtacggg tgtagacatt aaaccggctg gcaataatga ttccggagaa
    21181 tgccggcggt aatgcgcctg ccagtaccat catctgcaga tgttcgctgt tcaaatgaca
    21241 tgccatacca acgagaagca gtgccagtgg catcagaatc agcttcagga aggtgttata
    21301 agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg cagcgaatac
    21361 cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg ctgccggaat
    21421 ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacta ctggctcctt
    21481 tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc ctgaggaagg
    21541 attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag aaataattgc
    21601 taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg caaaccccag
    21661 gaatccaatg gtaggtgaac ccgcaattaa tgcacataca gctgcttctg catgggtacg
    21721 tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa taaccacaag
    21781 tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc gggtaataga
    21841 aacaaataga gccgcaggaa gagcgtagtt taataccagt ttattaaatg cgcgggcttg
    21901 atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga ttacgataat
    21961 aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa gggtcccctc
    22021 accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac cagcgacttg
    22081 ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt cgatgaccac
    22141 attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac gaagttcatc
    22201 tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt cataccttgc
    22261 atggtgcaac agatccgttg gtgatggtgc accagcgcca ctgagatcaa caccgtctcc
    22321 tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat atcgacaaat
    22381 cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg cgacaaccgg
    22441 agaaccagaa gtcacgctag caccgatggc atagcccata ccgatgccca tgacacccca
    22501 ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc gtgcattatc
    22561 cagggtattt gcaccttcat taactaaata aatatcctgg ttctcgcgca atacgtcgcg
    22621 cacagcactt aatgcattaa agtaatttaa tggttgagta tctgtactta atttttcatg
    22681 cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata ccagtggagt
    22741 cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg caatatcacc
    22801 aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa tatccagttg
    22861 aataaactgt gtatctgccg cccatccttt tttaccgtgt gccaataacc aattcagtcg
    22921 tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg cagctgccgc
    22981 agaaagtgga tgcgtatctt caaggatccc tttcgccata gacattggca ggaatggaat
    23041 ctgagtactt tcaataaatt cacgaagctg ttcatcagct tgtgaatacg ccgcgccttt
    23101 gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc tagtgactga
    23161 cttcgggcat ggcaataatg ctggcgacgg attttcaact ttaacaatcg tggttaacgc
    23221 ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat aaactccgcc
    23281 agggcgaccc gatacagaga cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg
    23341 ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt ggtccagctc
    23401 ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc taatcatgat
    23461 catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga ggaatcctgg
    23521 cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg ccgcagcggc
    23581 atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg cctgggcatg
    23641 gcgtgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa tattattctg
    23701 ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat ctgacatgat
    23761 taataacctc gaaattttta taaattggga gccagaagcc cccgttaata tcagatggcg
    23821 tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg caatacagca
    23881 gcggtatgtt cacctaatag cggcgcagct ttaatatccg gcgtaaaggc agagaatttc
    23941 attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc aacaacactg
    24001 ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac cggtgcacaa
    24061 ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt gacagtgtat
    24121 ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gtgcatgggc tgtactgtat
    24181 gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc ccagttttgc
    24241 tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt acatttcagg
    24301 atccagccag gctgaccgcc accacctgca ttaccaccgc ggggaactgc atcaccaaat
    24361 gtaccattcg gatactgcgg gtattcttcc agataaccca atttatcgag acgctgctgg
    24421 tcacgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat ggtgactcgt
    24481 tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc
    24541 attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc atcccaaaaa
    24601 cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc ttttacattc
    24661 acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg tggattgatt
    24721 tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg aaagttctcg
    24781 actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc cgctgttttg
    24841 gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag cgcatcgata
    24901 tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa cgccgggacg ttcaatttta
    24961 ataacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga ttgcacaccg
    25021 gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc tattagttaa
    25081 ttaaatatgt gttaatttag cgtttattta aggcgtaata tcttctataa ataacccgca
    25141 aaatatcgtc gtagctgcac tcgatatttt taaaatcctg tatcaagtgc tgaaaatcat
    25201 ttcatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa agaagaatat
    25261 gataaatatt gattcgtggt aatcagagag agtcgcaaca tttgtgatgt tattcttaca
    25321 actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt aaagcgtacc
    25381 ataaaacata acataataaa tgatttgtga tgacacggga ttattgggat ttactttgag
    25441 gaagataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga ccacaacctg
    25501 tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta cctcaatata
    25561 ctgcctgtca gcattagttc acaaacagtg cttcgctgtc gacgatgatt ccatcttccg
    25621 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
    25681 cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc attacgccaa
    25741 caccggccag gcgaagttct tccattgcgc cctttttatc ccctttcgcc attttttcat
    25801 tggcaatttt tatcgcggct tctttttcgg gagttgctac ataactctca gaaattccga
    25861 cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta cctggcttag
    25921 cgaactttgc ccattcggta ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt
    25981 cttttgcctt ttctggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
    26041 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgaa
    26101 cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag
    26161 atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct
    26221 ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga
    26281 gcaacggtca ttttgaaagg gttatcttac agttgtagtt attatcgcaa gaagtatttt
    26341 agtcttacaa ttgtaggaat ctgcttcctg tcgcagagaa ctattcttat gtaccacaaa
    26401 gagatgtcat ttattatatc gatgtaaata ttgattaatt atatgtaaat acagccataa
    26461 catcgtaggt tctcagtgaa ccattttcag gataactttc tatgaaagta aacttaatac
    26521 ttttcagctt atttttattg gtctctatta tggcatgcaa tgtttttgca ttttccattt
    26581 ctggtggtgt aagtgagagg agctataaag agactgaaaa aacatcagcg atgacaacca
    26641 cacactctac aaaacttcag ccatcacagg cgattttgtt taagatgaga gaagatgcgc
    26701 caccattaaa cctcacagaa gaaatcacgc ctacttatcc gacaaaggcg aattatctta
    26761 ttcatcctgt gcgatagcgt ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg
    26821 tgaacacctg cccaaatgag ccatcctgac ccactatcgc ctgccatacc ccaccataaa
    26881 gcgaatcatc aagccacaag ccggggcaag aaacataaat atccccgtca ctagttcatt
    26941 accgccagtt aaccacatgg tgaagtaata aatgagtgca gcgaggatca ccatgccgaa
    27001 gaaagaagag accgccgata ccacgttcag tacctgaccg atgtagccgc ttacgcagac
    27061 aatacacagg gcgagaaaca tccataaata aatcatagct caacctcctg ccgcgctaaa
    27121 aaacctgtaa cttcagtcac tttaatagta agacaatcct taacctctgg caatttttgc
    27181 taagcatcaa tgaaaacagc atgttaaatg caagactgtt gtgtacggaa aaatatttac
    27241 tttgcacgat taataatcag tagctgaaag cagtcagcga gtgataaagt acagataccg
    27301 cattcatgct cctcgtggtt atgtcctgac tagtctttac actctttaca ggaaccattg
    27361 tcgtacatga tggcccaacc aattgagtgt accgctgtcg acatggaatc ttctgcgata
    27421 caacaattcg tatctacaga aggtaactat gtttccacaa tgcaaatttt cccgcgagtt
    27481 tctacatcct cgctactggc tcacatggtt tgggcttggt gtactctggc tttgggtaca
    27541 gcttccttat cctgttctct gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt
    27601 cctgaaacgt cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc
    27661 tgcggaagaa cgcgagaaga tgattgccga aaactttcgt tcactcggca tggcgctggt
    27721 agaaaccggc atggcatggt tctggcccga cagtcgcgta cgtaaatggt ttgatgttga
    27781 agggttggat aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt
    27841 ccattttatg tcgctggaac tgggcggccg cgtgatggga ctgtgccaac caatgatggc
    27901 tacctatcgt ccacataata atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg
    27961 ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg cactgaagaa
    28021 aggtgaagcg gtatggtttg ctcccgatca ggattatggt cgtaaaggca gctccttcgc
    28081 gccgttcttt gcggtggaaa atgtcgccac aaccaatggc acctatgttc tctcccgtct
    28141 ctctggcgca gccatgttga ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg
    28201 tttgttcatc accccagaga tggaaggcta cccgacagat gaaaatcaag ccgctgccta
    28261 tatgaacaag attatcgaga aagagatcat gcgcgcaccg gagcagtacc tctggatcca
    28321 ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgttg tacatttaac aaactgctct
    28381 tactctttcc ctcctcaaat cggccataat aacctcatgg tttttatggc ttttttctta
    28441 aggttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact gtcgccgata
    28501 tgagacactt gtaagttact aaaagtaaat aagaaaatgt ctcttgtctc gtttcagttt
    28561 taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt gatgcccacg
    28621 gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta tcaggtgtaa
    28681 tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa tatttcttta
    28741 tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg atctaaaagc
    28801 aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg acgacggtgt
    28861 ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt agtaaaccgt cggcacggaa
    28921 catcgcttta atcccacgaa tcgcctgacg aatacggtcg cggttttcaa tcagtgcaaa
    28981 gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac aaaccttcgc
    29041 ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg gttccgggat
    29101 tttcgcccag acatacatcg aagccttcgg catttcaacc atccagcccg cttcatgcag
    29161 ccctttaacc aatacatcac ggcggcgttt gtactgttcg gcaatgtcgc gcacgcactg
    29221 ttgatcgccc tccagcgccg caatcgccgc cacctgcaac ggcgtaaagg tgccgtaatc
    29281 gtgatagctt ttaatacgag ccagggcgct gaccagcgtt ttgttgccaa ccataaagcc
    29341 gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga ctgccacatc
    29401 gcgtgcaccc ggtacctgca tgattgacgg cgctttccag ccatcgtaga cgatatcggc
    29461 ataggccagg tcgtggacca ccagcacatc gtagcgtttc gccagcgcca caaccttttc
    29521 gaagaactcc agctccacgc attgcgcggt tggattcgac gggaagccga ggatcatcat
    29581 cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga aatcgacacc
    29641 ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat aaatatggat
    29701 cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg ccagcatcag
    29761 atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg ggtcgatttc
    29821 aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg gaatgccgcg
    29881 tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt tttcgacgat
    29941 atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat cttcgccgcg
    30001 ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg ggagacgatc
    30061 aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag attaacgtta
    30121 gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa tagcctgatt
    30181 aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggctttaaat gaggaactcc
    30241 acttcaaaaa caggaaattt ttctacgcta acaagaaaat taccaccaat tttattaaca
    30301 ttataatttc aataaattta ccaaccagaa gtgcattatc aagagataca ttgctaatac
    30361 accatttatc tttcaatccc cttttaaatt tatggttttt cctcaaaaac ggcctgctgg
    30421 tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt cacccgtgca
    30481 cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc ttatctgcct
    30541 gttctttctc atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg cgcactcccc
    30601 aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca gcacctggtc
    30661 cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga tgtccggtgg
    30721 gattctgttt ggcccgtggg taggcatcat taccggcgtg attgcgggta ttcaccggta
    30781 tttaattgat atcggcggcg tgacggcgat cccctgcttt atcaccagca ttctggcggg
    30841 ttgtatatcg ggctggatca acctgaaaat ccccaaagca cagcgctggc gcgtcggtat
    30901 tctcggcggc atgttgtgtg agacgttgac catgattctg gtaattgtct gggcaccaac
    30961 taccgcgttg gggatcgata tcgtctctaa aatcggcatt ccaatgatcc tcggtagcgt
    31021 ctgtatcggc tttattgtgc ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc
    31081 cgcgcggcag gccaagctgg cgctggatat cgccaacaaa acgctaccgc tgtttcgcca
    31141 tgtcaatagc gagtcattac gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga
    31201 tgcggtggcg attactaata ccgatcatgt gctggcctat gttggcgtgg gtgaacataa
    31261 ctatcagaat ggcgatgact tcattagccc gactacccgt caggcgatga attacggaaa
    31321 aatcatcatt aaaaacaatg atgaagccca ccgcacacca gagattcatt ccatgctggt
    31381 gatcccattg tgggagaaag gggtcgtgac cggaacgctg aaaatttact actgccacgc
    31441 gcatcagatc acctcgtcat tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac
    31501 gcaactggag gtttcacgcg ccgagcagct acgtgaaatg gcaaataagg cagagcttcg
    31561 cgccctgcaa agcaaaatta atccccattt tctgtttaac gctctgaacg ctatttcatc
    31621 gtcaatccgt ctgaatccgg ataccgctcg ccagttgatc tttaatctgt cgcgttatct
    31681 gcgctataac attgaattaa aagacgatga gcaaatcgat atcaaaaaag agctgtatca
    31741 aattaaagac tatattgcca ttgagcaggc ccgctttggt gacaagctga cggttatcta
    31801 tgatattgat gaagaggtga attgctgcat tcccagcctg ctgatccagc cgttggtgga
    31861 gaacgccatt gtccacggta ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt
    31921 tgcagagtgc ggaaatcggg tacgcattgc ggtgcgagat accgggcacg gcatcgatcc
    31981 aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc aataaaattg gcctgctgaa
    32041 tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg catatccgcc gcctggagcc
    32101 ggggacggaa attgcgtttt acattcctaa ccaacgcacc ccagtcgcct cacaggctac
    32161 gttattgctt tgagccggag tgatattgtg aaagtcatca ttgttgaaga cgaattcctg
    32221 gcacaacagg aactgagctg gctaattaaa gagcacagcc agatggagat tgtcggcacc
    32281 tttgacgacg gtctggacgt gttgaagttt ttgcagcata accgcgtcga cgccattttt
    32341 ctggatatca atattccgtc gctggatggc gtgttgctgg cgcaaaacat cagccagttc
    32401 gcccataaac cgtttattgt gttcatcacc gcgtggaaag aacatgcggt agaagcgttt
    32461 gaactggagg cgtttgacta cattctcaaa ccgtatcagg agtcacgtat taccgggatg
    32521 ctgcaaaaac tggaagcggc ctggcaacaa tagcagacca gcagtacgcc tgccgcgacg
    32581 gtaacgcgtg agaatgacac cattaatctg gtgaaagatg agcgaataat cgtcacgcca
    32641 attaacgata tctattacgc cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt
    32701 gaatcctacg taatgccgat gaacattacc gaattttgca gcaaactgcc gccgtcgcat
    32761 tttttccgct gccatcgctc attttgtgtc aatctgaaca aaatacgcga aatcgaaccg
    32821 tggtttaata acacctacat tctgcgactg aaagatctgg attttgaagt gccggtcagt
    32881 cgcagcaaag tgaaagaatt tcgccagtta atgcatcttt aatcgggaac tttcatgaaa
    32941 gcaccaggct tgcccgccga tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt
    33001 aacccgcaac tactggggcg cgtctggttt gccagccagc ctgcctcgtt accggtgggc
    33061 agtttatgta ttgattttcc ccgtctggat atcgtgctgc gcggcgaata cggcaacctg
    33121 ctggaagcaa agcagcagcg aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg
    33181 gctaatttac cgatcaacaa caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc
    33241 tggcttgggt tatcgtttta cgatagccgc accacgtcgt tgttgcatcc tgctcgccag
    33301 atccagcttc ccagcctgca acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat
    33361 cttagccgtt cgccgctgga gcagaatatc attcagccac tggtgttaag tttgctgcat
    33421 ctttgccgca acgtggtgaa tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc
    33481 tatcacagca tttgtaactg ggttcaggat aattatgccc agccgctcac ccgcgagagc
    33541 gtggcgcagt tttttaatat cacgcccaat catctgtcaa aactgtttgc tcagcatgga
    33601 acgatgcgtt ttatcgagta tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg
    33661 cagaaatatc atctgtcaat tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac
    33721 tatttttgtc gcgttttccg gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt
    33781 tttcagggct aacgtcagaa ggttaattct gtttccagca gcgtcaggat actttgtgca
    33841 tcttgcgcgg caaacaacga ctggcggaag tttttattca ccagtttacg cgccagctgc
    33901 gagaagactt tcacatgatt catcccttcg ttagcaccca gcgtcagcat gatcaccagt
    33961 tcgacttcgc ccatttctga ctgccagtca accggtttcg ccagccgggc aatgctgata
    34021 ctggaatgac ggatccactg agatttggtg tgcgggatcg ctacgccaaa accaacgccg
    34081 gtggtgacaa tctcttcccg ctgccagaca tcttcttcca gctcaaacgg atgttcagtg
    34141 cgcccgttaa cgccgaggtt gccgcacagg aactggatcg cctgctcttt attgctaaaa
    34201 tcctgatcaa caaagatatt ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta
    34261 aaggcggtga gtaacgcttc aatttcctgc gcactgcggc attcacatgc ctgacgcgcc
    34321 agttcccgac acgcctcgct atccagttga cgaagctggc ttttcaccgc cggaatacgc
    34381 gggctactca tactcagctc atccaggccc agcccaagca gtagcggcag ataacggctt
    34441 tcaccgccca gttcaccgca aatgcctacc catttgcccc gctgatgcgc agtggtaaca
    34501 atctgctgca acatgcgcag gaacgatggc gtaatcgggt tatacagcgg cgatacgcgc
    34561 gggttattac gatcgaccgc atacagatac tgggtcatat cgttggagcc gatactgaag
    34621 aaatcgacct catcgcagaa gtgatcgatg atgtagcaca ccgacggcac ttccaccatg
    34681 atccccagcg taatcgtctc tgcatgacgc aggccatcgc gcttaagctc aacgatcgct
    34741 ttttggatct cgcccttcac ccataagatc tgatcgagac tgtggaccat cgggatcatc
    34801 aactgggcgt tgccaaaact ggcggcgcgc aaaatcgccc gtagttgagt gcggaacagg
    34861 ccagcaaatt ccgggtaaat acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg
    34921 ggaatattca gataaggaat gcttttatcg ccaccgatgt ccattgtgcg gaagataatc
    34981 ggcttgtcgc ccgctgccag tagcacctgc tggtaggctt caaattgctc ctgctcgtcc
    35041 ggcgcgctgt cgcgatccat atagagcatt tcagtacgga acagtccaac gccttccgcg
    35101 ccgttggcaa acgcgcctgg cgcttccaga gcggtgccga tattcgccgc aatatcaata
    35161 cgcttgttat cacgggaata ggccagctgc gcggcggcct gcgcctgttg tttttggcgt
    35221 ttatccgcca acgtctgtgc gacctgataa taaccgctca ccgcgtcatt cgggttaatc
    35281 gccagcacgc cgcactgggc gtcaagcacc gccggttgcc cggcataacg ggcaatcgca
    35341 tccagcggca ggccgctcag taccgggatc gccgaggcgc gggtcagaat cagggtatgc
    35401 gaggtgcgcc cagttttctc cagaatcatg cctgcaagat tttttaaatc gaggctcaaa
    35461 aactggcttg gggttaaatc ctcggctacc agaatagtcg gtttttccag caccagattg
    35521 ttgcgcggct tcagttccgg ccaggtgata tgcagcaact gttcgctaat atcgcgaatg
    35581 tcgctaacac gttcgcgcag ataatcactg gcagaagcag aaagtttggc gcaaacctgc
    35641 tccatattgc tgatgatcgc cgcccccagc ccttgatgct gttctgtcat cagacgacgg
    35701 atattgcccg cgaattcatc atcctgaatc agcgacaaat gggcgctgag gatagttttg
    35761 ctttcgccgt cacgctcacg caattgctgg ttcagttgct cggcaagcgt tgccaggctg
    35821 tgctccagcc gggtggaatc ttgcgcactg gcggggattg cccgataact gtcgaggctg
    35881 tcgctttgta acagggtcag cgtacccacg ccgacgccgc ttgccagcac attgccgtac
    35941 agtaaatccg ggttaaggcg gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc
    36001 tgcgtcggct gaacgctgtc gctgtcgata aagcgcacca ggatgtactc ttccagcacg
    36061 cgccgcgcct gctcttcatc gctgccgcta atgttcaggc tgcaactgtc attaaataag
    36121 gtgccagttc caatcaacgc cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg
    36181 ttgaaaaagg tgatttcgct ttgccactgg ctgcactgtt ctttaagttc ccacgccgga
    36241 cgagcatgta gaccgttagg caaaggacag agaaattgaa tcgttaacat aaccgctccg
    36301 ggttaatcag gagatcatct gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt
    36361 tgaataaggg cagataaaag ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat
    36421 gccgcgcaat gtccgtggcg ggtggcaggc cccattacca ctgtggggac gccagtaccg
    36481 gttaagtgca ccgctccgcc gtccgtgccg ccgttgctga acatatctgc ctgcaacggt
    36541 acgccaattt cagccgccac ggtttcgatc caggcggtaa gttttggcgg cgcaatcagt
    36601 gacttgtcgc ttaacaccag catcgggccg ttaccaatct ggcgatggtt agccgcgcca
    36661 taatcaaagt ttttcgccca gcaggcggta tccagcacaa tggcgacgtc cggcgacacc
    36721 gcgcgggtag cggtttgccc accgcgtaat cccacctctt cgctggaact tgccaccagc
    36781 cacacctccg caggcagttc ggcatcgtgc agctcacgta gtaacgtcac cagcagatag
    36841 caaccgaggc gatcatcaaa ggctttcccc atcacccgct ggtgagggag aacctgaaaa
    36901 gttgtatcaa aggtgacgcg atcgcctgga cgaattcccg cctgcatcac ttcgtcatac
    36961 gagcgcgcac caatgtccac gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca
    37021 agcaggcctg gaattttgca ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg
    37081 gcagccatgc gcacgttgcc aaccggcagc acatcaattg ccccttcgcg ggagatgcta
    37141 cgcaccataa atcccacttc gtccatatgg gcgcagatca tcaccttcgg acctgtcgat
    37201 tcattgaggc ggatcagcac cgatcccaga ccatcaaatc gcacttcttt ttgcaggcga
    37261 tccgcttctt ccagcaggat ctgccgcact tcctgttccg aggaggcgat cgcatctgcc
    37321 tcgctcaacg cttttaatag cgataaatcc attatgcctc tcccgtgagc aacactgttt
    37381 tcggcatggc gtagagcact tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc
    37441 gcacgccccc ttgccctggc aaataaatcc ccggctccac ggtcagtaac atgcctggct
    37501 gtagcgtcgt ggtgtcccgc ggtgaaaaac gcggatcttc atgaacttca atgccgatag
    37561 catgaccggt gttatgaccg aaatagtggc taaaacctgc ctcggtaatc acttggcgcg
    37621 cggcatcgtc aacctgctgg cagcgcacgc cggggcgaat tgcagagatt gctgcgagct
    37681 gtgcctgcag gacaatctgg tagacgttaa acagcgggtg agattcggcg ctcacccctt
    37741 cgccattcac cagcaaggtg cgcgtcatat cagagcagta gccctgatac agcgcaccaa
    37801 aatcgagagt gacaaactcg cccgctgcaa caatcttgtc gctggctttg ccgtgcggca
    37861 gcgccccgcg ccagccgctg gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct
    37921 ggcgcataaa ccactccagt tcagcggcta tctcgcgctc gctcatcccc gcctgaataa
    37981 agcggcgaat atgctccgct ccacgatcgg caatcccaca ggcgaggcgg attatctcca
    38041 cctcctctgg cgttttgatt tgccgcagca catccggcgt ggcgctaacc agtttcgcat
    38101 tgagttcaga cttccagcgg tgcgcggttt cccaactcac ctgctggcct tcaaaaccga
    38161 gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat agtggtaagc gtgttcgtcg
    38221 cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc cacctcaacg aaatagcgcg
    38281 aatctaccag aatgtgcgca ctttcacggc taataaccac atagcctgat ccggtggaga
    38341 tccccagatg cggctgtttg ttctgccgtg aggagagaag cactgcatcc agttgttgcg
    38401 ccttaagcca gtcgcgcagc gaagcgagta atgtcatctg gattttcctt cttacaggct
    38461 atcgattaac aatttgcctt tacggaacat catcagacgc aggaacacca ccatcagcgc
    38521 agttatgact gcccccagcg cgatccccgc catatagacg cccaggttag tgaccagcgg
    38581 ccatgcccag atagcggatt ccgggaacca ttgcactgcg cccagccaga cagcggcggt
    38641 tgagccgaca atcgcgccga ccatatacga cggaatggcg gtgatggggc tttccagcgc
    38701 aaacggaatc gcaccctcac taatccccat aaaggcaagg aacatcgcgg tcttaccctg
    38761 cggataaagt tgagcgttga acaggcgttt gccggttaaa cgacggtcaa taatggtcgc
    38821 cagacccaga ccaatcggag gaataacgat agcgatagaa cgagcggtga ccggtaatac
    38881 gtggtcagtg gtaaagctga aggcaacgaa acctgcggct ttgttgatcg ggccaccaag
    38941 gtcgattgct gtcgccgcgg cgatccccat tgcgtacatc agcgcgccct tctcacctgc
    39001 ggcagtcagc acagtacgga taccgccgtt gatccagccg ccaaacgggg tgatgacgta
    39061 gtacatcgcc agcatgacaa aaatggcgga aagaatcggt agcaaaaatg tggttttgaa
    39121 cgctaacagg aaatccggta actggatttt ctggttcatc catttcacaa ggtaaccggc
    39181 aacgatagaa ataatcagcg cgccaatgaa ggtggacggc accggcgaag aggtcgccca
    39241 ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc
    39301 gataaaacca gccgggaacg ccagtttgcc accgatagag ttcgccacaa aagcagcaaa
    39361 catcggaatg gcaaaaccaa acaacacgcc gccgaaggac tgcgacagcc aggcaaattt
    39421 cagtaaggag aggtcgaaac cggagaattt cccgctatta agagcatcca tgatgccgat
    39481 ctcagcagga attttaagcc agctataagc aatcagctgg ctgaaagcga ggatcacacc
    39541 gcccataatt aaggtcggca ccatgcgtga aatccctgac atcacatgct gcgggagttc
    39601 accccagaag ctgcttttag aggcctgcgg attcttaact gccgctgccg caccggatgc
    39661 gccaggcaca acggttgcac tgcgtttttt aatggccata acaattatcc ctgtcgaata
    39721 ttactgttgt tcagaggcaa tcatctcttc gatttctttg atgatgcccg cagcgttttt
    39781 aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgct cattatcttc
    39841 cggggtaact gccacggagt ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag
    39901 gcgattctgg ataccgtccg cgccctgggt ttcaattttc acttcataac cggcttctac
    39961 cgccgcttct tccagcgcct gagcggccat aaaagtgtga gccaggccca ttgggcaggc
    40021 acataaggca attagtttct tactcatcgt taattcctca ttcagtgata gttgacgagt
    40081 gaaattatga acgtgcaaaa caaatcgccg ttaccagaca aataatgcat ttactggaga
    40141 attaatcccg ctgtacggaa gtgtgacgca ggtcggtctt gtgaggggac acaaagaaaa
    40201 attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga tataacagga
    40261 aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc gagaagaccc
    40321 ggattgtcat ggacgatgag atacaccgga atatcataga catattcttt aaagcgccct
    40381 ttatcttcaa atgcggcacg gaaaccagag gctttgaaga actcaaggaa gcgcggcacg
    40441 ataccgcccg caataaacac gccgccaaat gtcccgagat tgagcgccag attgccgcca
    40501 aaacggccca taatgacgca aaacagcgac aacgcgcggc ggcaatcggt gcagctgtca
    40561 gccagcgcac gttcggtaat atcttttggc ttgagatttt ctggcaggcg gttgtcagct
    40621 ttcacaattg cgcgatacaa attcaccagc ccagggccag aaagcacgcg ttccgccgaa
    40681 acatggccaa tttccgcacg cagtatttcg aggataatgg cctcttcttc actattcggc
    40741 gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc gacatggacc
    40801 agatgcgcaa ccccaagccc tgttccggca ccgtaaaccg caataggttt accttcgacc
    40861 ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat cgccatcgat
    40921 acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt tttcatttcg
    40981 gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt aattgggcaa
    41041 gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata aacgcgaatg
    41101 accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga gatttcacca
    41161 ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc gactaatgca
    41221 tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc acactgtaaa
    41281 tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt
    41341 cacacattta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc aagctgggca
    41401 aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag ccagaaccat
    41461 gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta ttctgattgt
    41521 ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc cgggaactct
    41581 ggggatagcc caggattcac ctctctggat catcggtgta ttaacgctaa cgggtattgc
    41641 ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg cctgtgaacc
    41701 gctgatcggc gcaccagttc cgccctctgc gctaccagga cttatcgtag cattaattct
    41761 cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg tcaatatcgc
    41821 ccttgcggtt gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat
    41881 tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat
    41941 attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc tttttgcgcc
    42001 attaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc cccatttttc
    42061 actgcccatt gcgcattacg aacagatgga aatgaccgat attctcagcg gtgcaattgt
    42121 cgcggcgatt gccatcgcag caggaatggt cgccgtatgg tgcttaccac ggttgcacgc
    42181 gatgatgcat caaatgaaaa atccggtgct cgtgctaggt attggcggat ttattctcgg
    42241 tattctggga gttattggtg ggccggtttc gctgtttaaa gggctggatg agatgcagca
    42301 gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg taattaaact
    42361 tgccgctctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt
    42421 gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg ttccagcggc
    42481 aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg atggctggtt
    42541 aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc tgctctgtat
    42601 cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg tcaatcgtcc
    42661 gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctcttcttta tgatttaaga
    42721 gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag gccgctgacg
    42781 gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg ccgccctgat
    42841 ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat cgatcaaatt
    42901 acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact ggcgagatcg
    42961 tgattactgg catcgtaatt atgaatggcg caaaaaccgc tggtggcgtc atgataatgg
    43021 ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg
    43081 cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt aaacgcgtcc
    43141 tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc
    43201 tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt gccagatgcg
    43261 acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat ttagcgccac
    43321 gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc
    43381 catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg cgataccaaa
    43441 acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac
    43501 aataaatgac ggcagcatgg tgactgtacg acgcacccac agcgggatat ggaagcgaat
    43561 gaacccctgc atcaccacct gccctgccag tgtccccacc accgttgagg acagtccggc
    43621 agcaaccagg cttaatccaa agaccgttgc cgcagcatgg cttaacaacg gttgcagcgt
    43681 cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga acgccgccgc
    43741 agctgtagcc atcatcgcca gattgacaaa accggcaatc gtcatggcga tagccacatc
    43801 ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga
    43861 agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg ctgccaggaa
    43921 gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct gcgccaggtt
    43981 aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca gtaacccgcc
    44041 aatcactttc tccagcggtt tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc
    44101 gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt taaaaccgat
    44161 cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg cctgaaccca
    44221 atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca gatttttacc
    44281 ggtggcaatc cctagtttgg cagagaggag ctgaatcagc atcgccatca ggttggccca
    44341 aacgacaacc cacagtagct gatagccgaa gctagcaccc gcctgaatat tggtcgcaaa
    44401 gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta atgcgagcct
    44461 catcttgcgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca tcttgtgcct
    44521 ctaaaacata gcctttgcta tgtttcatgc tatgccaaac gagaatgatt atcaaattca
    44581 tttgaatggg ttgtggtgat ttctttgata gaccaggatt atgactacga aaagattgat
    44641 gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc agcttccata
    44701 cacaacatag cagaaatgta tgacagatca ctatttttga agcttgtcac aggacgtcat
    44761 tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg cacggaaatt
    44821 taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta ctggcacttg
    44881 ccgttgttgc gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt
    44941 atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg aactccgacg
    45001 ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc ggatttgcca
    45061 acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca ttcttcttcc
    45121 tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc cagcacattc
    45181 gcgtgttgcc ggtgatcatc cgcgcaattg gtttcctgct ctccaaagtc aacggcatgg
    45241 gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtct gaaaacttta
    45301 ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc atggctgcca
    45361 cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg ctggaaccga
    45421 aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga
    45481 tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac ctgcacgaag
    45541 gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa gttgccatta
    45601 tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg
    45661 ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg ggctacatct
    45721 tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa gtgggcagta
    45781 tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg cagaaaattg
    45841 cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctggtt tccttcgcta
    45901 acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta
    45961 acgtggtttc tcgcttcggt ctgaagctgg tttacggctc taccctggtg agtgtgctgt
    46021 ctgcgtcaat cgcagcactg gtgctgtaag accatacata aaaaagccgg ggataattcc
    46081 ccggtttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca aatacccttg
    46141 cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc
    46201 cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca caatcatcgc
    46261 atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga tgatatcagc
    46321 ctgcaaacgc tttaaccgtt cgtagttggc atatccagtg ccaaagtcat caatcgcaat
    46381 ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg cgttagaaaa
    46441 cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat aacgcttaaa
    46501 cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca ttaaattgac
    46561 tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca acaaggattc
    46621 cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg gcagaaactt
    46681 atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat cataaccttc
    46741 accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt
    46801 gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca tctcctctct
    46861 gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc agcttaactg
    46921 ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg cagcaccata
    46981 gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga tgttaaccat
    47041 atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt cactacccgg
    47101 caattgatac aacttttcgt tttcctgcat caacggcagc agcgtacggc aaattgagcg
    47161 gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa gattatcaat
    47221 gcgcaggcag caaaaactct tgcccgcctc ttgctccggc gcttgttcca acgcacgaaa
    47281 gttgggtagg agcgttaacg gatctgtcag cgcctgcaaa tgccagcggc gattaagcca
    47341 ttcactgcga tgataaatgc gcaccatata gagcaggcaa acgctaaagg aaatcagcac
    47401 cgcaagaata aatgccagcg aatattcggt ttccacccct tgcaaaaagt tctggttgta
    47461 attcagcagg caaagcgttg aaacagccca ggtgagattt aaaaacggat agcgaagctt
    47521 accgaccccg agggtaaaga tgatgaagaa aacgggtacc aagtaaccgg caataaagtc
    47581 gttttcataa ggtgtgcaca acagaagtag cagcacgctt aacgctgcca gccagcttaa
    47641 ggtaaatgcg cgtttctctt tgcccaacga cggagcgata tccctgcgcc acaatatctg
    47701 cgcaaagtgg ggacttacaa tcatgcgagt gagatagtag aaaagcatgt tgtaaatcag
    47761 cacagcggtg aaaaggctta gcaaatcaac gaccgtgaaa atggcatccg catcgccgaa
    47821 aaaggtggat atctttagcg ggaaatcaaa gaagctcccc acaagataca tgctgcattt
    47881 gataccgatc ggcgtcacca aaccaagcca gaacagacgt tgccagacat atcggctggt
    47941 caatccataa cgccaacgcg tccccagctg ccagcgtaat attgcgcagg cacacaacac
    48001 gacaaacgtc tggctaccga gcatgaccgc agtctgtaaa aaagagagat gaaagttcca
    48061 cagattggtg caaaacatcc cgcataaaac gggaacgaca ccgcgccagc caaagataaa
    48121 caagattgac agcattacgc acagtggcat ccacgccaga aagatataac tggaatggat
    48181 aatggcaagt ggcgaaataa aacgggatag ctgaataagt accacggtga gcgtaaacgc
    48241 tagtgtgaat atcttgatat tttttatcag gttatgctcc acaaacatga gtacactact
    48301 ctcactgatc ggggttttca ggcacacaaa taataagttt attcgcatcg cgaagcaaac
    48361 aggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcgctaaccc
    48421 ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag
    48481 cgagggttgg aaatttggtg gagctaagca ggatcgaacc gctgacctct tgcatgccat
    48541 gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca aatttgctgg
    48601 gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
    48661 cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat
    48721 catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga
    48781 aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac
    48841 ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata cattcaggga
    48901 attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc
    48961 cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc
    49021 gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc
    49081 aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt
    49141 gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca
    49201 tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc
    49261 aaataatatg aaaagattac gcaacaaaat gaccaccgaa gaactggctg aatgcctcgg
    49321 tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa cggaaaaatt
    49381 tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt
    49441 tattcagaac acgccagcct tccataacac accaatgttg atggaagctg aagagagaat
    49501 tgctgaatac gctcctggag cacgcgcccc ggcgtatcga caaatcatca acgcgataga
    49561 taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg
    49621 caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt
    49681 atctcctgcc gtttatcttt ttacacgcta attactgctg attttcgcgt tcagcaataa
    49741 aatccagcgc tttgttgata cgctcgatac tgcgggtctt accaattgcg tgaacggtaa
    49801 catccagtgc tggagactgc cccgcaccgg ttacggcgac acgcagcggc ataccaactt
    49861 tacccatacc cacttccagc tcatccgccg tcgcctgaat agcgtgatga acgttttcag
    49921 cggtccagtc agtaatcgcg gccagtttgt cacgaaccac ttccagcggc tgacgcgcta
    49981 ccggacgcag atgttttttc gccgcatcgg catcgaactc agcaaaatct tcgtagaaat
    50041 aacggcagct ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc agtttcacca
    50101 gatcagccag ctgcgggccg ttacgggtat cgatattttc ctgctcaatg tgccactgta
    50161 agtgagtagc aacatactcc ggcggcagcg cgttaatgta gtgatggttc agccacagca
    50221 gcttgtcggt gttgaacgca ctggcagatt tgctgacggc attcaaagtg aagtatttga
    50281 tcatctcttc acgagtgaag atttcctgat cgccgtggga ccagcccaga cgcaccagat
    50341 agttcagcag tgcttctggc aaataaccgt catcacgata ctgcattacg ctgactgccc
    50401 cgtgacgttt ggacagtttt ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa
    50461 ccggcaccgg cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat
    50521 ggtcttcgcc acggataacg tgggtgattt ccatatccca gtcatcgaca accacacaga
    50581 agttataggt tggggaacca tcggtacggc ggataatcag atcatccagc tcctggttgc
    50641 tgaactcgat cggaccacgg atctgatcgt caaaaacaac agaaccttcc tgcggattag
    50701 caaaacgcac gacacacggt tcatcatcag catgatgctc atggctgtgg cggcagcgac
    50761 cgtcataacg cggcttctca cctttcgcca tttgctcttc gcgcagcgct tccaggcgct
    50821 ctttagagca atagcattta taagcagtgc cctcttccag catctgatcg atcaccgcat
    50881 tgtagcgatc aaaacgtttg gtctggtagt acggaccttc atcccactcc aggctcagcc
    50941 agttcatgcc atccataatg gcttcgatag cttccggcgt ggaacgctca agatcggtgt
    51001 cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag
    51061 cagtacgcgc gccgccaacg tgcagatagc ctgttgggct tggcgcgaag cgagttttga
    51121 ttttcatgaa atggccttac gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat
    51181 tgagtgggcg atattctatc actcccgcct gattcctcaa tgtagttcgg gttgcaacct
    51241 tacgcattat tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg atcatcttgc
    51301 ttaaatttac gacgaacgaa caatttcttt agaaaatgcg ttgactcatt ttgaactctc
    51361 cctataatgc gactccacac agcgggggtg attagctcag ctgggagagc acctccctta
    51421 caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc gttagctcag
    51481 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
    51541 aatgtaaaaa aagcgcccta aaggcgcttt tttgctatct gcgatactca aagattcgaa
    51601 gctgccgcag gttcgagttg agcgcagcga aacaacggag ccgctcgcgg cgacggcccg
    51661 aagggcgagc gaagcgagtc atcctgcacg acccaccaat gtaaaaaagc gccctaaagg
    51721 cgcttttttg ctatctgcga tttgcgaaat tgcctgatgc gcttcactta gcagacgact
    51781 atttccggca attcctgtct cctcacctac tgtgtcaatg cagccaacag cttaaccatc
    51841 gcgggcgtca cctgctgtgt ttcataaaca atatataaat ctgcagggat gcgctgtttg
    51901 agcggacgga aaatgacacc tggccagttc atttgtgcgt agctgtccgc tatcaatgtg
    51961 ataccaatac ccatactgac catggcgagt accgtttgcg gttcattaac ttcgcgaata
    52021 acaaccggtg aaaatcccac ctgctggcaa actcgctgca aaaaatccca gtcagtgtac
    52081 acgggcggca ttgtaacaaa atactcgtca cgtagcgctt ccagcggaac ggtggaaaat
    52141 gatgagagat gatgctcttc aggcatcgcc accagaaacg ccgattcatg caaccgtaag
    52201 ctggtaaaac ctgtcggtgg ttctgtcgcc attcgccaga tcccggcatc gagttcacgt
    52261 cgttccagca gggccatttg catcgcgggc atcttttcgc gaaaaagaac ttcaacgtta
    52321 ggattttccc tgaggaatcg ccgcataacc gggcgcatcc gtccccacat agccgttccc
    52381 actacgccga gttcaatccg ccctgcttct ccccgaccta tttgttcaac ccgagccaat
    52441 acattattag cattcaccag caatcgacgc gattcttcca tcaagatttt gcccgcgtgt
    52501 gtcagtacga cgctgcgcga atggcgaata aaaagctgcg tgccgagttg attttccagc
    52561 tctttaatat gaatactgag cggaggctga gacatattta aacgcgctgc tgcgcggcca
    52621 aaatgcaact cttccgctac ggcaagaaaa taacggagca acttaagatc tgttctgtat
    52681 acgcgttcca taattaagtg ctccatataa taaatacatc gtattaaatt atcatggcat
    52741 aaagtatttc accaccagat atccgacata cataaacaca atggataata tacctggtaa
    52801 tacatttggt acaggaatag gcaagcgcac gattgtaaac aaagcaccaa tgccaaaacc
    52861 aacgccggaa gaaaataaaa tatctttcac taattaacct ttatcataaa agcagctctg
    52921 aagagcagag ctgcgaatcc ttttaatgag ccaccgctcg atgctttatc ttttcagggt
    52981 catgattata tttaaaccca aagaaaaata tcactgcgag aaaaagagca tatcctgcaa
    53041 acaccagcca gatagtttgc cagtctttta cgccatccac cgaaaagtaa tctactgcca
    53101 tgccactcag aatcgagcca acccatgcgc cgacaccatt taccatggtc ataaagagcc
    53161 cctgcgcgct ggcacgaatg ctggaatcaa cttcctgttc gacaaatacc gaaccagaaa
    53221 tattgaagaa atcgaatgca cagccataaa caatcatcga cagcagcagc aaaataaatc
    53281 cggttgttga cggatcgcca taggcgaaga agccaaagcg cagcgtccag gccaccatac
    53341 tcatcagcat gacggtttta atgccaaatc gctttaaaaa gaatgggata gtcagtataa
    53401 agcccacttc tgccatctgt gaaactgaca gtaaaatgga gggatatttc accacaaaac
    53461 tgtcagcaaa ctccgggtta cgggcgaaat catgtaggaa cggattacca aaaacgttgg
    53521 taatttgcag taccgcaccc agcatcatgg caaagaggaa aaagatggcc atgcgtggat
    53581 ttttaaacag cacgaaggca tccagaccca gcttgctggc aagcgatgtg gtcgcttttt
    53641 tctccgcaac cggaatcttc ggcaaagtca gcgcataagc cgacagcagc aatgacgcac
    53701 cggacgcgat atacagctgc agactactca attccagatg cagcaggctt actgcccaca
    53761 tcgcgacaat gaaccccacc gtaccaaaaa cgcgaatggg cgggaaagcg gtcaccgggt
    53821 caagccctgc ctgggcaaga caggaataag agacgctgtt cgataacgca atagtcggca
    53881 taaacgccat cgcattgact aacatcaccc aaaacatcat atccggatca gttacggatg
    53941 ccgcataaaa aagtacgccc gcacacacca ggtgacacag catgtatgca cgttctgcgc
    54001 gcagccattt gtctgcgatg atccccatta taccaggcat aataatcgcg gcgatccctt
    54061 tggaactgta aaccatgcca acattagcgc cggtgaaatg aagggtatta atcatgtaag
    54121 agccgagggt aaccagccag ctcccccaga taaaatattg caaaaaggac attaccttta
    54181 agcgcatcgc gatgctcata atcgtttcct tgtcgcatca tttggcctca cccgcgaggc
    54241 cgtttcttat tgtttttctc aggcaatttt gcgcagaaag ccacaaataa ggttgatgaa
    54301 gttctgctta gagagctccg cagccgccag cgtttgcgca tgtgacaatt tcacatcgct
    54361 taaaccttcc gccatattgg taatcgcaga gaccgcaacg actttaaggt cgcaatggcg
    54421 agctgaaata acctcaggca ccacagacat accaacaaca tccccaccaa taatttgcat
    54481 catgcgaatt tccgccgcag tctcgaaatt cggccccgga tacgagacga acacgccctc
    54541 cgtcagaggg aaaccctctt ctttcgccac tttttgtaac agtgcgcggt attccgcatc
    54601 gtaggcattt gccagtgaga agaagcgctc tccaaaacga tcatcgttaa gacccaccat
    54661 cggcgtaccg ggcatggtgt tgatatgatc tttcaatgcg accaggctgc ctgcccccac
    54721 ttccggacgc agcgagcctg ccgcattggt gcagaacagt agctcgcagc ccagcagctt
    54781 aaaggtacga attgcgtcag tcataatggt cattccacga ccttcgtaga aatgtccgcg
    54841 acctttcata cataccaccg gtacgccctg gagatggccc agcactaact cccccgcatg
    54901 accatgtacc gtacttaccg ggaagcccgg cagcttttcg taggaaattg cgacagcgtt
    54961 ctcaatctga tcggccagcg cgccgagccc ggaacctaaa ataaaggcca ctcgtggcgt
    55021 gaaatcaggt ttataagtct tgataatatc tatgcaaaac agcgggttat gagaaaattg
    55081 aacctgagac atacatatcc tttttctgta gggcggaatc taacgccttt ttatagcgag
    55141 gttgtcccgg ctcttaccaa tacacttttc tcacgcattc aatagcgatt cgatatcgat
    55201 ggatttatat cttttctcta ttaatccacg gtaaaaactg tattggcgat attaattcct
    55261 ttgcattaac aatatgtcgt ttttagcaaa ccactttatt aagcaatcta ctaataacga
    55321 tcggcatagg gaattatgct atgaaaaaac atcttttaac tctgacactt tcctctatat
    55381 tagcgatacc tgttgtatct catgcagaat ttaaaggcgg ttttgcagat atcggcgtgc
    55441 attatctgga ctggaccagc cggactaccg aaaaatcgtc gaccaaatca cacaaagatg
    55501 attttggtta tcttgaattt gaaggtggcg ctaactttag ttggggagaa atgtacgggt
    55561 tcttcgactg ggaaaacttc tataacggtc gccataataa accaggtagc gagcagcgtt
    55621 atacctttaa aaataccaac cgtatttacc tgggtgacac cggatttaat ctctacttgc
    55681 atgcgtatgg cacctacggt tctgcgaatc gcgtgaattt ccacgacgat atgttcctgt
    55741 acggtatcgg ctacaatttc accggcagcg gttggtggtt caaaccgttc tttgctaagc
    55801 gttatacaga tcaaacctat tacactggcg acaacggcta tgtcgccggc tgggttgcag
    55861 gttacaactt tatgctgggc agcgagaaat tcactcttac caactggaac gagtacgagt
    55921 ttgaccgtga cgctacctat gcagcgggta atggcggtaa agaagggctg aatggcgcag
    55981 ttgcactgtg gtggaatgca acatcacaca ttactacggg gattcagtat cgatatgcgg
    56041 atgacaaact gggcgaagat ttttaccagg atgcgataat ctactccatc aaatttaact
    56101 tctgattgat tactgccggg gctcccggca gtatgaattc actctatatc tgatacaacg
    56161 gatccccttc ccgaccagcc attagcgcat tgccctggtc tcgtaccacg tcccataacg
    56221 cttcagcggc ggtggaaagt gaacggttct tcctgcgcac cagcatcaac tggcgttcaa
    56281 caaccggcgt aatgcgcttt accaccagtg ggctaccttc aggcaatggt agcgccagtg
    56341 cgggaagaat actgatgccg atgcctgcgg ctaccatcgg gaacagcgtc gccggatgtc
    56401 caatctcctg cacaatattc gcctgaatac cattgcgcgc cagcgctgcg tcaatcagcg
    56461 gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc tccctgtaat gcctgccagg
    56521 gtacataatc ttcgacggcc aaagcgctat cgcgatggca aagcagaaag aaaggctcgg
    56581 aaagaatcgc ttcacattgc agatcgccca ctgggcctgg atcgatgacg atgccaaagt
    56641 cgacatctcc ctggcgaata ctttccatca cccactgctg cggacgatcg tgcaggacaa
    56701 actgaatatc tggatagcgg cgatggcttt cagcaatgca ttgcggaata agatgcgcgg
    56761 aaatggtctg gctggcagcg acccgaactt ttccgctcag ttgttgcccc atacgcccgg
    56821 tatcgcgcaa cgtgctgttc agttcatcca gcagtcgctc aagacgcaaa gccagctgct
    56881 gccctgcatc tgtaagcacc acttcacgcg tggttctgtc cagcaggcga acaccagtat
    56941 gattttccag ttccttcaca ctgtgactca ctgccgactg gctcaggccg atacgctctc
    57001 ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa aacctttaat tgttttaaag
    57061 aataattcat ctgttttctt catgaatgga tgcaataaat caattttatt tctcaaactg
    57121 gaagaagcac aatagaacca tcgatcatct ggagtcttta tgaaactttt tcgtatcctc
    57181 gatcctttca ccttaaccct gatcacggtg gtgttgctgg cctctttctt tccggccaga
    57241 ggcgatttcg tccccttctt tgaaaatctg accaccgcag cgattgccct gctgttcttt
    57301 atgcacggcg cgaagttgtc gcgtgaggcg attattgctg gcggtggtca ctggcgactg
    57361 catttgtggg taatttgcag caccttcgtg ctgtttccga ttctgggtgt actgtttgcc
    57421 tggtggaaac cggtaaatgt cgacccgatg ctctactccg gttttctcta cttgtgcatt
    57481 ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa tggcgggcgg taacgtcgcg
    57541 gcggcggttt gttctgcgtc agcatccagc ctgctgggga ttttcctttc accattgctg
    57601 gttggtctgg tgatgaatgt tcacggtgca gggggcagcc ttgagcaggt cggtaaaatt
    57661 atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt cccggccgtg gattggtgac
    57721 tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc agacgtccat tctgttggtg
    57781 gtttatacag cgttcagcga agccgtcgtt aatggtatct ggcataaagt tggctgggga
    57841 tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg ctatcgtgat tgtagttaac
    57901 gtctttatgg cacgccgact gggcttcaat aaggcagatg aaattactat cgtcttttgt
    57961 ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa acattctgtt ccccacatcg
    58021 gtgatcggta tgatggtgct gcccctaatg attttccatc agatccaatt gatggtctgt
    58081 gcggtgctgg cgcgtcgata caaacgccag accgaacagt tacaggcaca gcaggaaagc
    58141 agcgccgata aagcttaaag cggacgcttc aggggctgga ccagttgcgt cagcccctcg
    58201 gttttaatca gcagcgtgat ttgcattagc tcaccgagct tccctgccgg gaattcatct
    58261 ttgcgggcaa accacagcag atactcctcc ggcaggtcga ttaagcgacg ccctttatat
    58321 ttgccaaacg gcattatcgt attggctatt tcaatcagct gctctttttc catctcagct
    58381 acccagcaaa cgcagcattt ccgcttcgtc gatgacttca atgcccagtt cctgcgcttt
    58441 cgccagttta gaacctgccg cttctccggc aatcaccaga tcggttttct tcgacacgct
    58501 gcccgcgact ttcgcgccca gttcaaccag tcgagcttta gcgtcatcac gcgacatctg
    58561 gcttaagctg cccgtaagca ccacggtttt accagcaaac gggctgtcaa tctcttccgc
    58621 gttgataacg atcggcgcag gccagtgaac accttccgcc aacagctcgc tgatgacatt
    58681 gcggttgctt tcttcggcaa agaagttgtg aacgtgggat gcaacgacaa tgccaacatc
    58741 aggcaccttt tgcagctctt caatcgaagc ggcttccagc gcttccagcg tgccgaaata
    58801 tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg atgccaagtg catagaggaa
    58861 gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta accacgtttt gtgccgattt
    58921 tggccccata cgctccagtc cggtcagttt gcctgcggtg agtttgaaca gatctgccgg
    58981 agtatggaca tattcttttt caaccagctg atcgatgatt ttgtcgccca ttccgtcaac
    59041 atccatcgca cggcgggaaa caaagtgttt cagcgactct ttacgctgcg caccgcaaat
    59101 caggccaccg gtacagcggg caaccgcttc accttccaca cgctcaacgt cagaaccaca
    59161 taccggacaa tgcgtcggga atacaacctc acgagtatct tccgggcgtt cagaaagcac
    59221 gacgttaacc acctgcggga tcacgtcgcc agcgcggcga atcaccactt tatcgccaat
    59281 gcgtaaacca agacgttcga tttcatccgc attgtgtaag gttgcgttac tcaccagcac
    59341 gccagcaaca tggacaggtt ccagacgcgc aacaggcgta atcgcgccag tacgcccaac
    59401 ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc gccgggaatt taaacgctac
    59461 cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc tgctgttcca gtgagttgac
    59521 cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg cggtcttctt ccactttgtg
    59581 atagaacgcc agcacttctt ccgccgattc acaaagcgtt acccgatcgc tgaccggcaa
    59641 cccccacttt ttaaattgca gtaaacggcc aagatgagta tccggcagct cgccaccttc
    59701 cagaacacca acgccatagc agaaaaaagt gagcggtcgc ttcgctgtaa tacgcggatc
    59761 aagctgacgc agtgaaccag ctgccgcatt acgtgggtta gcaaaaactt tcccgcccgt
    59821 gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc ggcaggaaca cttcaccacg
    59881 cacttccaga cgcgccggga tattctctcc gtgcagcttc agcggaatag cgcgaatagt
    59941 acgcacatta gacgtgatat cttccccggt ggtgccatcg ccacgggtcg cggcactgac
    60001 taaaacgcca ttttcataca gaatactgac ggcaaggcca tccagcttca gctcacagca
    60061 ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca cgtttgttga aagcaagaaa
    60121 gctttcttca tcaaaaacgt tatccagtga cagcattggt acttcatggc gtatctggct
    60181 gaaagccgcc agcggcgcag cgcctacacg ttgggtaggc gaatcaggcg taatcagttc
    60241 tggatgtttg gtttccagct cgcgcagttc gcgcatcagc ctgtcgtatt cagcgtcggg
    60301 aatttccggc gcatccatca catgataaag atattcatga tggcgaagcg tcgttcgcag
    60361 ttctgtcagt tgttgttcga ttgattccat atcgcaccat caatgctaaa aacccccgac
    60421 aagcgggggt tcgaagagga gttaatttgc cttaagtgta tcaggcgttg gcgtctttga
    60481 cttcgcggat gatgtcctgg tactcgcgca atttctgcgg agtcatcata cggcgctgat
    60541 cgtcaagcac gacaccgccc acttcatcgg caatatgctg cgcagattgc agcatcagct
    60601 tgaagttctg cagctcgtca ccgtaagacg gtacctgcat aaagatggtg acacccggag
    60661 tagtgaaatc cttcatttca ggatcaaagg ttcccggttt caccatattc gccaggctga
    60721 ataacgccgg gccgctgcca tccgggctaa gatgacgatg gtaaatattc atatcgccaa
    60781 aaatgaagcc cgcttgttga atgctgttaa gaagcagttc accgtttagc tcgctaccgt
    60841 gatgcgccgc gacgttcatg ataatcaccg cttctttgcg cttcggttta tccataactg
    60901 gagccggttc cgctacaggc tcaggctgtg gtgccgctac gggttctgca ggctggaaag
    60961 cctgttgtgc cggttgcggt gctgaatgca caggctgcgg cgctggcgcg acctgtggcg
    61021 aaactggctg ctgcaacggc tgttcaggct gcggctgata ggcaggctgc tgcaccggct
    61081 gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggctgc tggaccggtt
    61141 gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac ggacgagcag
    61201 cctcatgctc ctgagcgtta gccggggcat gattcacgcg gtgaacacga acctcaccaa
    61261 cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc
    61321 gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc cagaaaccat
    61381 gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga cgcaaatcct
    61441 gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat ttactcacta
    61501 agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag cacaaagata
    61561 gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat tgcgcaatta
    61621 cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa ggagctcatc ctgattatgg
    61681 tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc
    61741 tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc aatattttgt
    61801 tgatgggggg cgcattctgg tggctcttta cacagctcga tgtctggatc ccgactctca
    61861 tgagttacgt tccggactgg ctacaatggc tgagttatct gttgtggcct ctggctgtca
    61921 tctctgtgct gttagtgttt ggctatttct tctccacgat tgctaactgg attgccgctc
    61981 cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct acaccgccag
    62041 ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa
    62101 aatttgcctg gtatctgccg cgcgcaattg tattgctaat tctttactta atccctggta
    62161 ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg ttagccatcc
    62221 agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg cgcaccgccc
    62281 tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg tttacgatga
    62341 tcccgctgct taatctgttc atcatgcccg ttgccgtttg tggcgcgacg gcgatgtggg
    62401 tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt tattttgtaa
    62461 accgtttgtg tgaaacaggg ggggcttatg ccgcccctta ttccatcttg catgtcatta
    62521 tttcccttct gtatatagat atgctaaatc cttacttccg catattctct gagcgggtat
    62581 gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa gatttttgaa
    62641 gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga
    62701 cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg ccgtatcggt
    62761 gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt
    62821 gaaccgacca gcggtaatac cgggattgca ctggcctatg tagctgccgc tcgcggttac
    62881 aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct gctgaaagcg
    62941 ttaggtgcaa acctggtgct gacggaaggt gctaaaggca tgaaaggcgc aatccaaaaa
    63001 gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca attcagcaat
    63061 ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg agatctggga agataccgac
    63121 ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac tggcgtcagc
    63181 cgctacatta aaggcaccaa aggcaagacc gatcttatct ctgtcgccgt tgagccaacc
    63241 gattctccag ttatcgccca ggcactggct ggtgaagaga ttaaacctgg cccgcataaa
    63301 attcagggta ttggcgctgg ttttatccca gctaacctcg atctcaagct ggtcgataaa
    63361 gtcattggca tcaccaatga agaagcgatt tctaccgcgc gtcgtctgat ggaagaagaa
    63421 ggtattcttg caggtatctc ttctggagca gctgttgctg cggcgttgaa actacaagaa
    63481 gatgaaagct ttaccaacaa gaatattgtg gttattctac cgtcatcggg tgagcgttat
    63541 ttaagcaccg cattgtttgc cgatctcttc actgagaaag aactgcaaca gtaatgccag
    63601 cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt ttttgtggcc tgcttcaaac
    63661 tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca gactaattat
    63721 tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt ttatgatttg
    63781 gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca gcggtcaaca
    63841 cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct
    63901 aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac actaaaccta
    63961 taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg aacggtctgc
    64021 acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct gaaattactg
    64081 tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag actctgggcc
    64141 tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag aaagcggttg
    64201 aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt taaaaatcag
    64261 tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta tcgctttcgg
    64321 taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa tttctgccga
    64381 ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat cagcccagct
    64441 ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga
    64501 agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag ccctgattaa
    64561 agataagcac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc
    64621 cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg
    64681 taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg ccattcagga
    64741 tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc tgaacctgaa
    64801 gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct ctatcatggc
    64861 gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa
    64921 tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc caaccaacga
    64981 agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag cagagcttgc
    65041 taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag tgtgcgctaa
    65101 cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct
    65161 gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag aagaacagtt
    65221 tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg ttcgtaccat
    65281 ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag agaacccgtt
    65341 cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa gagatcctgc gcgatcagct
    65401 ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc cgatgatcat
    65461 ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac aggaactgcg
    65521 cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc
    65581 tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta tcggcaccaa
    65641 tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt cacaccttta
    65701 ccaaccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg cttctcatgc
    65761 tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg ctacacttct
    65821 gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc cgcgcattaa
    65881 gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc
    65941 tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag aaaaaacaat
    66001 ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg ggtttgttcg
    66061 ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt gagatcattg
    66121 ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa
    66181 aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc gcgcctgtag
    66241 acggtaccat tggtaaaatc tttgaaacca atcacgcgtt ctctatcgaa tctgatagcg
    66301 gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc gaaggcttca
    66361 agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt gaatttgatc
    66421 tgccgctact ggaagagaaa gccaagtcta ccctgactcc ggttgttatc tccaacatgg
    66481 acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa accccggtta
    66541 tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc gcccatcggc gccatttttt
    66601 atgcttccgc cagcggcggc aaaatcaatt catcgctctc atgctgctgg gtgtagcgca
    66661 tcacttccag tacgcgcaac cccgcacggt gcaccgcatc gtttaacgcc ttccctttca
    66721 gcaaaccact gataagctga gcacaaaaca ggtcgccagt ccctttcagg tcggttttta
    66781 cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac caccaccacc tgcatctcct
    66841 gattttcttc attaccggag gcgctggtaa tcaccaccca ttttaatgtg tctgaaagca
    66901 gactttttgc ggcagcaatg gcactgtcga gatcgcggca atttttaccg gtcaggattt
    66961 ccagctcaaa gatattgggg gtaattccct gcgccagcgg cagtaaatat tgtcgatacg
    67021 cctcgggaag gtcaggtttg acataaattc cgctatcaat atcgccaatc accggatcga
    67081 ccatgatcaa taggtcagga tggtctttgc gtagcgcagt cagccactcg gcaaggattt
    67141 tgatttgcga tgccgttccc atatagcccg tggttacagc gcgcagctgg cgcagcgcat
    67201 cacgctcctg aagcgcacgc aaataaccgc taaaccattc atccggaatc gcaccaccgt
    67261 agaaagtgtc ataatgcggc gtattgctca gcaataccgt cggcacggca aaaacattca
    67321 gaccgttctg tttgatagca ggcacggcaa tgctgttgcc cacgctgccg taaaccacct
    67381 gcgactgcac ggcgacgata tccgcctgca gcgccctgct cttatcgtta aacaacaaca
    67441 aactactcat ttaatttttt ctccttgccg atgatcctca tcgtattcca accgaaactt
    67501 tacctgattc tggcagtcaa atcggctatc acaaaacaag gataaggtaa ttcaatgaag
    67561 aaaatcattt gtctggtcat tacactacta atgacactcc ccgcttacgc gaagttaact
    67621 gcccatgaag aagcccgcat caacgccatg ctggagggat tagcacagaa aaaggatttg
    67681 atatttgtgc gcaacggtga tgaacatacc tgcgatgaag cggtttctca tctgcgtctg
    67741 aagctgggca atacccgtaa ccgcattgac actgccgagc agtttattga taaggttgct
    67801 tcgtcgtcat cgattaccgg gaagccgtat attgtgaaga tccccggtaa gagcgatgag
    67861 aacgcacagc cttttttaca tgcgctaatt gcgcagacgg ataaaacggt gcctgcgcaa
    67921 taatcttaaa tccccgcccc ctggctaaaa tgctcttccc caaacacccc ggtggaaagg
    67981 tagcgatcgc cacgatcgca gatgatcgct accaccaccg caccagggtt agcttttgcc
    68041 acccgcagtg ctccggcaac cgcgccgccg gagctgacgc cacagaatat tccttcccgc
    68101 actgccagtt cgcgcatggt gttttctgca tcgcgctgat gaatatccag cacctcatcc
    68161 accagagaag cgttgaaaat ccccggcaga tattccgcag gccagcggcg aatgccggga
    68221 atactgctgc cttcttccgg ctgcaggcca acaatcgtta ctggtttgga ttgttcgcgc
    68281 ataaagcgtg aaacgccggt gatagtgccg gttgttccca tgctggagac aaaatgggta
    68341 atgcgcccgc cggtttgctg ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg
    68401 ttatcgggat tattaaactg atcgagcagc tttccttcgc cacgattcgc catctccagc
    68461 gccagatcgc gcgcaccttc catgccctgc tctttggtga caagaatcag ttccgcacca
    68521 taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag caatttcatg
    68581 cgatagcctt tcagcgcggc aatcattgcc agcgcaatgc cggtgttacc actggtggct
    68641 tcgattaaga catcacccgg tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa
    68701 agtgccgcac gatctttcac cgaacctgcc gggttattgc cttccagttt taaccacact
    68761 tcactgccgt tatccggccc cattcgctgc aacttcacca gaggcgtatt gcctattgtt
    68821 tgttctaatg tactcacgat ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc
    68881 gtcttatcag gtctacaggt tacaaacctt gccattaaaa agcccggatc gcgggaagcg
    68941 cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag caagttcctc
    69001 atctcgggtt ttgatacgct cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa
    69061 taaacgctcg ccacgctgcg gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt
    69121 gtaccacccc agcggctgca ccactaattg ggtgtagtga cctttcgggc tggcttccag
    69181 tacctgtacc ggcagcggcg aatcgagact ggtacggcgg ctgatatcca cttcccacgg
    69241 gcgcaggaag agatccaccg gaccctgata cgcaggtgtg tagcccagcg gccagcgatg
    69301 cgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca cttcgcccat
    69361 aaattcgagt acaaaacggg tcgctggttc gcgccatacc tgattcggcg catcagcctg
    69421 ttcaatattc ccctgactca tcaccactac acgatcggct acttcggtcg cctcttcctg
    69481 gtcgtgggtc acgaacacgc tggtgaattt cagctcttca tgcagttgac gcagccagcg
    69541 acgcagctct ttacgcacct gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat
    69601 ttgcggttct acagccagcg cgcgcgccag cgccacgcgc tgtttctggc cgccggaaag
    69661 ctgtgccgga taacgatctg ccagatgagc aagctgcacc atctccagca attttgtcac
    69721 tttcgctttg atggctgcgg cattcgggcg ctcgcgacgt ggcagcaccg tcaggccaaa
    69781 agcgatattg tcgaacaccg tcatatgacg gaacagcgcg taatgctgga acacgaaacc
    69841 gaccttgcga tcgcgcgcgt gcaggcggct gacgtcggtg ccgtggaagc gaatatgccc
    69901 gctggtttga tgctccagcc cggcgataat gcgcagcagc gtggttttcc cggaaccgga
    69961 cggccccagc aacgcgacca tctgacctga aggaatatcc agtgagatat cgttcagcac
    70021 ctgggtgcga ccaaacgact tcttaatatt ggcaatctca atgctcatga tgttcctcct
    70081 gctgtgcgcg tttatcctga ttctccaggc gccactgcaa catacttttt aaaaacaggg
    70141 tgataatcgc catcagcgtt aacagtgccg cagcggtaaa ggagccgacg gtgttgtagt
    70201 cctgctccag caattcaatc tgtaacggca gcgacagggt ttcgccgcga atcgagccgg
    70261 aaaccaccga caccgcgcca aactcgccaa ttgcgcgggc gttggtcaac accacgccat
    70321 aaagcagcgc ccagcgaatg ttcggcaatg tgacgcggcg aaacatctgc cagccagacg
    70381 cgccaagcaa aatcgccgct tcgtcttcct ggctgccctg gcttaacatc accggcacca
    70441 gctcgcgcac cacaaacgga cacgtcacaa agatggtgac cagcaccatt ccgggccagg
    70501 agaacataat ttgcagatta tgttcatcca gccagccgcc cagcgggccg ttagagccgt
    70561 agaacagcag atacaccaga ccggcaacca ccggcgatac ggcaaacgga atgtccagta
    70621 gcgtcagcag taactggcgt ccagggaagt taaagcgcgt caccagccag gccagcagaa
    70681 tgccgaacac caggtttacc ggtacggcaa tcagcgcgat catcaccgtc agccagatgg
    70741 cgtgcagcat gtccggatcg gccagattct gtaaaaccgg catcagcccc ttgctgaatg
    70801 cctgcacgaa gatgtaaatc atcggcacca gcaggatgaa cgctgaaacc agcatcccga
    70861 tgccaattag aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc ttcaattggg
    70921 taacttccgc cattaatgac ctaccacacg ccgaccaaag cgactttgca gagtgttaat
    70981 tgagaacagc agcagcaggg atgccgcgag gatcaccgaa gcaatcgcgc tcgccgccgg
    71041 gtaatcaaac tcctgcaagc gcacaaaaat catcagcgac gtcacttccg tcttccaggc
    71101 gatgttcccg gcgataaata tcaccgcgcc aaactcacca agactacggg taaacgacaa
    71161 cgccacgccc gccaccagcg ctggagaaag ttccggcagc accactttgc agaaactctg
    71221 ccagcgcgtt gcaccaagcg tttccgccgc ttcttcatat tccgggccta actcttccag
    71281 caccggctgc acggtacgca ccacaaacgg aatgctggta aaggccatcg ccaccgcaat
    71341 accaagccag gtataggtga ctttgatatc aaacttcgcc agccattcac cgtaaaaacc
    71401 gtttacggaa aagagcgagg ccagcgttaa accggcgact gccgttggca gcgcaaaggg
    71461 taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgataacgtg ttaaaatcca
    71521 cgccatcagc agaccgaaaa cgccgttaaa aatcgatgcc acaaacgccg acagcagcgt
    71581 cactttgtag gccgcgacca cttgtgggtt ggtgatcacc tcccagtact gcgcccagct
    71641 catctgagcc agctgcatca ccagcgcgga gagcggcagc agcaaaatca ggcacacaaa
    71701 cagcagactg gtgccgaggc ttaaggtaaa gcccggcagc acgcgtcttg atgaaactgc
    71761 aaacatcagt tacgccccgc cgctaacagc ttgtctaact cgccgccgct ggtgaagtgg
    71821 gttttcatca cttccggcca ggagccaaat ttgtcttcca cgcggaacag ctcggtctgc
    71881 gggaatttat ctttcagttt gtccatgact tccgggttat ttacgcggta gtaatagtcg
    71941 gtgatgatgg tctgcgcctg cgggctgtag agccagttca ggtaggcttt tgctgctttt
    72001 tccgtaccgt tggcctgcac gtttttatcg acccacgcca ccgggaattc cgccagaata
    72061 ttggttttcg ggatcaccac ttcaaagccc tgtgcttcat actgtttacg gatgttgttc
    72121 acttccgatt caaagctaat cagcacatcg cccaggccac gctcggcaaa agtggtggtt
    72181 gcgccacgac cgccggtatc gaacacttca acatttttca ggaactgggt cataaactgt
    72241 tcggttttgg ctttatcgcc accgtcagct ttatccgctg cgccccatgc cgccagatag
    72301 gtataacgcg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc
    72361 accaggtcgt tccagtcgtg gatattcttc gggttgccct tacgcaccag gaagcccatg
    72421 gtggagtaga acggcgagct attattcggc aggcgcgact gccagtccgc cgggatcagc
    72481 ttgcctttgt catgcaggat ttgtacgtca gtcacctggt tataagtgac aacgtcggct
    72541 tttaagccct gtaaaatcgc cagcgcctgt tttgatgacc cggcatgaga ttgtttaatc
    72601 gtcagtttgt cgccgccgtt atctttggcc cattgctgtt caaacggcgg gttcagggcg
    72661 gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc ctgtacatgg
    72721 cctgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa gttaacggcc
    72781 attgcgcacc cttatatatt taatgacttt ctaatagcca tcatatttat aacggacgtg
    72841 aaaggagtaa cggttttata taccgtttgg tgatttggaa gttgaaaagg gaataagaac
    72901 cgggtttaaa acgaaatcct ccccacacat gcagggagga aacagaggtg aatcagatac
    72961 cgacgctaac cgtctccggc agtgtgctgc cgccatcaat cacattctgt gtaccggtta
    73021 aatagctgga ttcatccgat gcgaggaagg ccgccagttc gccgacttcc agcggatcgg
    73081 cgaggcgacg cagcgggatt gcttttgcca tatcagtcag caccgactct ggatcttccg
    73141 ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg gacgtacccc gggcagatgg
    73201 cgttaacgcg aataccggat tgcgcgtact ccaccgccag cgattttgtc aggccaacaa
    73261 tcgccgcttt cgttaaggcg tacgccgttt cacccggatc ggccaccata tcaccagtga
    73321 ctgaagacat catcacaatg cgaccatctt tgcgcgcaat catctctggc agcaccgcct
    73381 tcgtgacgtt ccatacgcct ttaatattga tgtcaatatg gaaatcgcga tcttcatcgc
    73441 tcatatcgag gaaactgccc agacgacaaa cgcctgcgtt attcaccagg atatcaatgc
    73501 gcccttcttt ttccttcgcg cgtttgatag ctgcggctac cgacgccggg tcacgcacat
    73561 cggcgacaac cgccgtacag cgatgaccac gaccacacag ttcgtccgcc agcttttcga
    73621 tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa gttctggcaa
    73681 ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc gtgagtttac
    73741 ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc tgaaaataca
    73801 ggataaacaa aatattgctg cggatcagct cactaattca ggaaaatctc aatccagtcg
    73861 ctgctttatg catcatttca ggcgttgtgt tagctcactg ctgcgctgaa tcattttcaa
    73921 tgactcgaca tcattgagtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    73981 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    74041 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    74101 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    74161 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    74221 cgtgtcggct tcgcaggcca cgcgatatcc aattttcatc agtttgaacg acagatcgcg
    74281 ccccaccagc gccgatccgc ccaggcccgt tatctgaata aattgcgctt tactgatgac
    74341 atcaatgatt ttttgcagcc gcgcgtagtc gaacaacgcg caggtttgct ccagcgccag
    74401 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    74461 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    74521 taacgccata cgcaattcag taaatccttg tgcgccgagc ttctgggcaa acttcacaat
    74581 actcgactgg ctgatgccga gctgtttcgc catcttgcgc gaagagacgg attgcagctc
    74641 actcacgttg gcctgcagaa agtcagcgat tttttgctcg ttttcggtga attcactccc
    74701 cgcattgctt attttcgtca ggtacaacat cttccactct cctgggctgg cagcataaat
    74761 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    74821 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    74881 aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc
    74941 tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac
    75001 cgtaccgctt gccgttgagc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca
    75061 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    75121 ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaactgg gtctggtggt
    75181 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggcg ctgaagatag
    75241 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    75301 tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca
    75361 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agtgccgttt ccaccaccgc
    75421 cgagtttgcc attacaccga ttgtaggtgc cgaagtggtc actggttctt cgcgaatgaa
    75481 agcaggcaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc
    75541 tggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    75601 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    75661 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    75721 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    75781 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    75841 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    75901 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    75961 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    76021 aaaagcgatg agcgagctgc tgggcgaggc accggtacag gatgccgcag aaatcgccgc
    76081 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    76141 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg
    76201 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    76261 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcactttcc tggtgattct
    76321 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    76381 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    76441 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc
    76501 gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    76561 gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat
    76621 gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa
    76681 tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    76741 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    76801 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    76861 ggcgcaaccg cacagtgcat tacgcagtca ggtacgcggg gcgattattc ccggtctgct
    76921 gggcgttggt gaaccgttga tttacggcgt caccctgccc cgcatgaaac cgtttattac
    76981 cgcctgttta ggcggcgcag cgggcggttt gtttatcggc ctgatagcct ggtggggtct
    77041 gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    77101 ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg
    77161 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    77221 tcatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    77281 caaaataccc tgttctgaca gaaagttcgc cagagaaagc agggttcaac gtcgaacggc
    77341 ttaaccagat ggatcgctgg attagccagc aagtggacgc cggttatccc ggcgtaaacc
    77401 tgctgatcat taaagataat cagattgtgt atcgcaaagc ctggggagcg gcgaaaaagt
    77461 acgatggcag cgtgttgatg gaacagcctg tcaaagccac caccgggacg ctgtatgacc
    77521 tggcctcaaa caccaaaatg tacgccacga acttcgccct gcaaaagctg atgagcgaag
    77581 gcaaactgca tcctgacgat cggattgcga aatatattcc ggggtttgcc gatagtccaa
    77641 atgacaccat caaaggcaaa aacacgctgc ggatttctga cctgctgcat cacagcggcg
    77701 gtttcccggc ggacccacaa tatccgaata aagcggtcgc gggcgcgtta tattcccagg
    77761 ataaaggcca gacgctggag atgatcaagc gcacgccgct ggagtatcag cccggcagca
    77821 agcatattta cagtgatgtc gattatatgc tgcttggatt tatcgtcgag tcggtcaccg
    77881 gtcagccgct tgaccgctat gttgaagagt cgatttatcg cccgctcggc ctgacacata
    77941 cggtatttaa cccgctgctg aaaggcttta aaccgcagca aattgccgcc acggaattga
    78001 acggcaatac ccgcgatggc gtgatccatt tcccgaatat ccgcacctcc actctctggg
    78061 gtcaggtgca cgatgaaaaa gccttttatt cgatgggcgg cgtttccggg cacgcaggtt
    78121 tgttttccaa taccggcgat attgcggtgt taatgcaaac gatgctgaac ggcgggggct
    78181 atggcgatgt gcagctgttc agtgcggaaa cggtgaagat gttcaccacc agctctaagg
    78241 aagatgccac ttttggcctc ggctggcgcg tgaatggtaa tgccaccatg acgccgacgt
    78301 ttggcacgct ggcaagcccg caaacctacg atcacactgg ctggaccgga acggtgaccg
    78361 ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca cattcgccgg
    78421 ttgccgatcc gcaaaagaat cccaatatgt tcgaaagcgg tcagctgccg attgcaacgt
    78481 atggttgggt agttgatcag gtgtatgcgg cgttaaagca aaagtaataa aaaggccagt
    78541 cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag cgccatcaac
    78601 ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa acgcaacatc
    78661 gcatcacgct taccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc
    78721 agacgcgcgc agtaggcgca gaagtacaga ccgtgaatgc cactggcagt gccgtacggc
    78781 aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtgagg
    78841 tgagaggttt ccggacgttc gtcgccgtcg atctcttcgt tggcctcttt ggtgcgcccg
    78901 atcatcatct cctgatcgtg aacgctcatc cggttgagct gcttcaggtt gtgttcccaa
    78961 cgctggacaa acacatagct gccgcccgca tccacgccgt ctttgataac cgccacttcg
    79021 cgacgcgtct cttcacccgc cgggttttcc gtaccgtcaa caaagccgct caggtcacgc
    79081 tcttcaaccc aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct
    79141 tccatcgccg cctgggcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc
    79201 aacacatcga actgggtggt cggcgcaagg cctttaccgt agcccggaaa atctttcagc
    79261 tcttctgccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc
    79321 gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt
    79381 ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc
    79441 gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat
    79501 aataatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt ttcgcggcgg
    79561 cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca
    79621 gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg aactggtagc
    79681 taatatgttg actgccttcg actttacatt cgacggcacg attatcatca ccatcagctt
    79741 tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac
    79801 cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc
    79861 cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc accacgttgc
    79921 cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcggcaatgg
    79981 cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg ctcactttgg
    80041 tggacgaccc aaaccagttc cacgggttag cggccgacca gttaactgac gacatcgtgg
    80101 aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcaagcga tttcatgtca
    80161 cttcctttgg ttattcaata acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat
    80221 tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc
    80281 acaatatccg ccactaccag cgccagaccg ataccgctaa cggattcacc gttcagccac
    80341 agccacggtt gccagcaaag taaaaccacc tgcgccaaca gcaacagaaa atagagcaca
    80401 tgccaagtgc gaggaaatgt agcccgccga ccgctcaaca aaaacgccag caccgccgga
    80461 atgcctggaa tcagccccag ccagaaatta tcgtgatcgg gataaaacag atttagcagc
    80521 gcagtaccct gctcgcgcga cgcaccggca atgacaaaca gcacccaggt tcgcgcctga
    80581 agcaatagca caagccagaa gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg
    80641 acaggatgga actcagtact cttcatcttc aatcaaacgc ttacccagac tcagcacgtc
    80701 ggcgtgttca tatcccaggc gttcatacat tccgagcacc atgtcgttat cttccggcac
    80761 attgatctga attttcgggc agccacgagc aatcagcttt ttctccagcc gattaagcaa
    80821 cgcattggca atcccacgcc cacgaaactc tggatgcacg ccaagataat aagcagaccc
    80881 gcgatgcccg tcataaccgc ccatcaccgt tccgaccact tcactgttta cctcagcgac
    80941 caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg tccatttccg gatcgttcca
    81001 cggacgcagc aagtcgcaac gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg
    81061 aaatacgcgt atctccatgg tattcgttac ctttttgcgg gttaaaaggc tgattatggc
    81121 gtgaacggtc gaattagcca atatctgacg aaaatcggtt gaaaaagtgg cataatgggg
    81181 agttgtcaac tattgaaatg aaaagtaaaa caattctcaa catcaaaccg tcgtaacgga
    81241 ttacgcgata cgatataaca tctggaactt tattattaca actcaggccg tatgagcact
    81301 tttaaaccac taaaaacact cacttcgcgc cgccaggtgc tgaaagccgg tttggctgcc
    81361 ctgacgttgt caggaatgtc gcaagccatc gccaaagacg aacctttaaa aaccagcaac
    81421 ggacacagca agccaaaagc caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca
    81481 ggccacggcg gaattgatac cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg
    81541 gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta atcatgggat tgatgcgcgt
    81601 ttaacgcgtt ctggcgatac gtttatccca ctttacgatc gcgttgaaat cgcccataaa
    81661 catggcgcag atctgtttat gtcaattcat gccgatggct ttaccaaccc gaaagctgcc
    81721 ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg
    81781 tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa aggcgactga caaggatcac
    81841 ctattgcagc aagtgctgtt tgatctggtg caaaccgata ctattaaaaa cagcctgacg
    81901 ctcggctcgc atattctgaa aaagattaag ccggtgcata aactgcacag ccgcaacacc
    81961 gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc
    82021 tcgtttatca ccaacccgga agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa
    82081 atcgccacag cgattgctga aggcgtgatc agttatttcc actggttcga caaccagaaa
    82141 gcacattcga aaaagcgata agttatgaaa cccgacgcac accaggttaa acagtttctg
    82201 ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc
    82261 gaagatagtt ggcagcgcga agctggcggc ggcgggcgta gtcgggtgtt gcgtaatggt
    82321 ggtgttttcg aacaggcagg cgtcaacttt tctcacgtcc acggcgaggc gatgccagct
    82381 tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca
    82441 ctggtagtgc atccgcataa cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt
    82501 attgccgaaa aaccgggtgc agagcccgtc tggtggtttg gcggcggctt cgatttaacc
    82561 cctttctatg gttttgaaga agacgccatt cactggcacc gcaccgcccg tgacctgtgc
    82621 ctgccatttg gtgaagacgt ttatccccgt tacaaaaagt ggtgcgacga ttacttctac
    82681 ctcaaacatc gcaacgaaca gcgcggtatt ggcgggctgt tctttgatga tctgaacacg
    82741 ccagatttcg accactgttt tgcctttatg caggcggtag gcaaaggcta caccgacgct
    82801 tatttaccaa ttgtagagcg acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt
    82861 cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg tctgggatcg cggcactctg
    82921 tttggcctgc aaactggcgg gcgcaccgag tcgatcctga tgtcaatgcc gccactggta
    82981 cgctgggagt atgattatca gccaaaagat ggcagcccag aagcggcgtt aagtgagttt
    83041 attaaggtca gggattgggt gtaactccct cacccccatt cccgcatccg ctgatgcaac
    83101 gtcaacgacg gcttctcggc aaacagctgc tggtaatccg tggcaaattg ccccagatgc
    83161 cagaatcccc actgcatggc ggcgtctttt accgttgtgc tttgcgacca cgggcttatc
    83221 agttcgcggc gtacggcgtt caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct
    83281 aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga catgcagttg attacacaag
    83341 tccagcaccg tcaccggctc agacatattt tccagcacat attcacgggc gcgggaaagt
    83401 aatctacggt aactctgatg actgatgctt tccgccgtca ccattggttg cgcgtcttcc
    83461 agcatcgccc ccatcgccat tagcaaatta tcccccagca cttttcgcac tgcaggctga
    83521 tggagatttt ccgggttctc gcaaaacgtc gccagcgcct gttggacaaa gccccacagc
    83581 gcggctttat gctgctcttt cacttccagc gccgactggc tacgcagcat atgtaatacc
    83641 cgatccgggt tatgcaaaaa gttagcctgc cgggtgatga catcttcaga aagcaccacg
    83701 cccaggatcg tgtaatcatc cggcgtgctc agttcaaatt cagtgccacc agggcgggtg
    83761 gcgatttccg cgcttcccag acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc
    83821 ggaatgccaa accagaacga gttcggccag accaggcacg actgacgcag cgccagaccg
    83881 gtgtattcac gaaaaacctg aatatcatcg agtagaattt ccgtaaattc accatgaaac
    83941 ttgcccggat gcagctgatc gtaaatctgc tgccaggcgg taatcgttaa agcatgttca
    84001 tagacatccg ttgtctgtcg ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc
    84061 aggttttcgg gtaagggttc atgataaaga tggtgcaaat tggctgtacg ggtctttttc
    84121 atgatgttaa tgccgggtgt tgtaggacac ccggcacctc cgacaggtta atggggcttg
    84181 agacgataac gactactgcg tttacgtaac gtcccggcag aaaagagctg ttcgagcgca
    84241 tttctggctt tttccagcgg ccagccaaag tgggcagcca cttctcctgc cgtcattccc
    84301 tgacgtactg atgttaacag cgccaacaac tcgtcagcag attccgcgac gataactggc
    84361 gcgtcgggtt cccttacggg ttgcttcggc tggcggttaa agccagtaac cagccactgg
    84421 gtgtcatcgt ctggccgacc gatctccttg cggctgatga cacgcccggt acgcatcgcg
    84481 gcagcacaac ctgcgtccag cgccgcacga cacgccgcca gatcgccttc caccaccagc
    84541 gttaagcgac cagggtcaag cacttcgtga ctgagcagac gaacgttagc tgctttgagc
    84601 atggcatccg ctgcatctat cgcggcgacc atgccgtcca cttccagcaa tcccagtgca
    84661 ttgatcatcg gcaacctccg ttacgcacgc tggattggat tacgcgcgat ttccagcact
    84721 gcatcggtaa aggcgttaca ggctgcttta cacgcggcct ggctaccggt taaaaatgct
    84781 gccgagtagt tggtttcaga cggtggcggg acataggtca ccagctgtac gtcggcagat
    84841 ttcaacgctg catcaatgcc gtaggtcgct tccagcggcg gtgccaccag atacgccatc
    84901 ggatcgccaa gcgtgatccc ggcggttgat gagagataag aaccggtacg cgaaactaca
    84961 tgtgccagga aggcggtgtt ttctgcgtca ttggcccact ggaaagctgc gccattttca
    85021 atatgtgcaa ccatcgcatc cagaccggca cgcacttccg ccgggtttgg cccaccaagc
    85081 ataatcagca cttcaccggc agtcggtgac gggccgtgtg ccgctcccgc atacagcgag
    85141 cgaccatata ccacttcgac catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtg
    85201 acgtcgtcag aatcggcaga aatcagcccg aggctacgaa tatgcggcgg caattttagt
    85261 tcacgcgcaa attcagcgtt aacggaggca atcacccgca tggcggttac cgacggtcga
    85321 atcaaatcta aagctggcat gatgcctcct tatcgggtca tgttgatgcc ggatgctttc
    85381 tgctccagca tacgtttcgc caaatccaca atgacggctg cggcttcaac cggcggcgtg
    85441 ccgccctggt gaatgttaga gatacaggtg cgatcggcct caacggtggt cgccatacgc
    85501 ggcgagtaaa cggcgtagca ggagaggctt tctgactgcc ctaaccccgg acgttccccc
    85561 accagcagga tcaccacttt cgcgccgagg atctcaccga tctgatcttc aatcttcacg
    85621 cgaccataac gaacaaagaa tggcgtgccg actttcagcc cggcctgttt caggcccgcc
    85681 atcagcggcg gcaggatctc ttcgtagttc acggtgatgg catcagttga caggccatca
    85741 gaaataacga cctgtacgtc cggattagca acacactgtg ctttcagcgc ttcaacagct
    85801 tctgcacaca ggcggcggcc catatccggg cgagtcaggt agagattttt gtcgctaatt
    85861 tcggagcgta cttccagcaa gccctgcgct ttcacccact cttccggcac ttctttcagt
    85921 acggtatctt tcgaacgaga gtgatcggcc aggaaacgca gcaacgcctg cgtacgcgga
    85981 cgcggaccgg cacgaccggt acaaacgcgg gccacggtgc tgcgacgcag ttctgttaat
    86041 acgtctgcgc gatgcgggtt ttcaacacca atccacgctt ttgcttccgc agaacctaaa
    86101 tccagcgcgc agctttccga ggtcaccggt gccgcacagt tggtggtggc gcactttgct
    86161 tctgacggcg cgggggccgt ttgtcccatt gacgccatca cgctgcgtac aatttcttca
    86221 atctgttttt gatccatgat gtgttatctc cgcgtcatca gaagaatagt gacggatcgc
    86281 ccgcccgttt ggtcaggcga ccgtttgcca taatgcccat gctttccagc cagcgttcaa
    86341 actccggtga cggacgcagg ttgagtaact gacgcacagt ggcggtatcg tggaatgcgg
    86401 tggtctggta gttgagcatg atgtcatcac ccagcggcat ccccatgatg tagttgcagc
    86461 ctgcggtggc gagcaggatc atcaggtttt cgttgaggtt ctggtcagcg tcagcgtggt
    86521 tggtgtaaca gcagtcacag cccatagaga tgccgctcag cttgcccata aagtgatctt
    86581 ctaagccagc acggataatc tggcggtcgt tgtagagata ctccggccca ataaagccga
    86641 ccacggtgtt gacgataaac ggatcgtaat gacgcgccag cccatagtta cgtgcttcca
    86701 tcgtcacctg gtcagcgccg aagttagcgc cagcggatag cgcagagcct tgtccggttt
    86761 cgaagtagag gcagttttcc ccggtgatac ggttgaactc tgcacccact gcgcgcgctt
    86821 cgtcgagcat cgccagttcc acgccaaact ctttcagccc tttttcgctg ccacagatac
    86881 tctggaaaat cagtccgcca ggcgcgccgc gacgaatcgc ttcgatctgg gtggtgacgt
    86941 gcgccagtac gcagccctga gttgggatgt tgaatttgtc gatcacgccg taaatggtgt
    87001 ccaacacgcg gcttaagttt tccacgtcgt cagttaccgg gttaacgccg atcaccgcat
    87061 cgcccacccc aaaggaaagc ccttcataga tttgcgcagc gatactttgc acgtcgtcac
    87121 gggtatcgtt cggctgcaaa cgggcgctga aggtgcccgg aataccaatg gtggtattgg
    87181 cctttttgat taccggcatt ttcttcgcgc cgtagatcag gtccgcgttg gagcaaatct
    87241 tcgctaccgc cgcgaccact tccgaggtca gccctttgcg ggtaaaggca atgtcgtcca
    87301 cgctggtttc atcgctcagc acatactcac gcagttcgct gatgctccag tttttaatct
    87361 ggttgtaggc cgtttcgtta acatcgtcct gaatcagccg cgtcacgcag tcatcttcat
    87421 aggcaatcac cggattattg cggatgtccg ctacggtcat ttccgacaac acctgctttg
    87481 ccgccacgcg ctcctgtgag cttgccgctg caacgcccgc cagcacatcc cccgaacgca
    87541 gttcgttggc tttagccagc acctctttta catccttaaa ctgatataca ttgccgaaca
    87601 atgtggtctt tagtttcata agtcgttccc tcaggaagga aatgcgagtg atttcaccgt
    87661 caccggcaca accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg
    87721 gacaatcact tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat
    87781 gcccagagct ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa
    87841 acgcgcgacg aaatcgacca gcgcgttgat gaccgtcagt accgcggcgt aacgcacagg
    87901 cagcgaggcg ggaagcgcca gcacgtacgc gtcagttttg ggatcaagat ccagctgaag
    87961 cagcgcctgt tgccaggcac tcaccagatc cgtttcatca atcgggatcg ccaccggcaa
    88021 attgcgcagc ggcagttgta cgccctccag ccagattgtg ctgccagaga gcgaaagggt
    88081 atgtgcaccc gcgccaatca ccgtggcgcg tacggtttgc gccggaaact gcacattcat
    88141 ctcacgcagg cgcggatggt catgcagcgc cgttgccaga agtgggccaa tatcggcaaa
    88201 acagaacggg tcggcgggct ggtggcgata acattcgccc acgcctccag aaagcgtaat
    88261 gatttcaggc gtaacacctg cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg
    88321 cgaaagcgtt ccgtcaatca cttcgacaat cagcgccgcc atccgccggg taacctgcac
    88381 cagctgcgcg ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact catccacaat
    88441 catctgcccc ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg
    88501 accaccgacg ttgagacagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt
    88561 cgcggtgcca ccgccgatgt cgatattcag tacccgacac agccgttgtt cagaaagggt
    88621 ttgcgcccca gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcaac
    88681 gacaaaatcc cccagcgatt gagagagcgc catcaccgcc gggcgagcat tgcgggtttt
    88741 cgcgctttca ccggtgatga tgatggcacc agaatcaacg ctttccggcg caatacccgc
    88801 agcctgatat tgctcgagta ttaaggtttt cagttccgct tcttttaaac cgccctgttt
    88861 atcgacaggg gtaaagaaca ccggactttg ccagctaatt tcgcgtttaa tgaattcgta
    88921 gcgcggcacc tgcgacaccg ccgcacggtt aaccagctcc aggtgggaga aaatcacctg
    88981 ggtggtggtg gtgccgatat cgataccgac tctcaatagc tggcgagtgt tcacgattgt
    89041 gcctccgctt cggttttagt cgcggtcgcg tcttcttttg gcaccagcat catcgccacg
    89101 ccaatcgccg ttacgccgcc gatcaacttg ccgacaatca tcgggaagat catggcgttc
    89161 atgttggcag cggcgaagcc taagtggtcg cccagggcga aagcagcgga aacggcgaag
    89221 gcgcagttga tgactttgcc gcgggtatcc atctgcttca tcatgccgaa catcgggatg
    89281 ttgttggcaa gcgttgccac catgccggct gccgcgatgt tgttcatatt cagcacttta
    89341 ccgacgctca tcagcggttt ttcaaaccag cgagtcagca gcagcaccat tggatacgcc
    89401 cctaacagca cgcaggagat agaaccgata acttcaatgg cgcgcatcac ttcaccgggt
    89461 ttatcgccag gggccataaa gataggatcc agtccgggga tcagttccca gccaaggagg
    89521 aatttcacta ccgcagcggc aagaccgagg gtgatcaatg ccacgaggaa tttggcgaag
    89581 atctggaagc cgttgatcat tttttccggg atgaatttca gccccagcgc caccagaatc
    89641 gcgacaatga tcaccgggat catgttcatc aggatcaggg cgaaagtgaa ttccaccggc
    89701 tgaccgttga tctgcacacc ggagtacata gcaaccagac caccagcaat acagccaatc
    89761 ggaatggtca caatgcccgc cagcacgccg agcgccagat aacgacggtc agaaggttcg
    89821 ataatgccga gcgccaccgg aatggaaaac acaatcgttg gccccatcat cgacccgaga
    89881 attaacccag agtatagcca cgctgctacg tcgccgcccg ccagctcttt ggcgaggaag
    89941 aagccgccca tatcgcacgc cagcagtgtt ccggcgaaca tcgatgggtt agcgccgagc
    90001 atttcgtaaa ccggaataat taccggcccg agtacgtgag ccagtaccgg tgccagcgcg
    90061 gtcataccga ccatcgccag gcccagtgcg cccattgcca taaagccttc ttcgaactga
    90121 ccgcctgatc cttcgatact tttaccgaac ttaccgagga aacgagcaga accgccgaac
    90181 tgcgacagga tcctgtctac ggcagctatc agcataaaga acatcatgat gtacatgatg
    90241 atttcgttaa ttcccatcgc ctttactccc tgttagttgt tatttattgg cggatgcggc
    90301 gtaaacgcct tatccgccct acatgtgcaa tcccgaaggc cggatcagac gcggcaagcg
    90361 tcgcatccgg catttattgc gccgctgcgt acaggccgac aatctgctcc aggctggcgg
    90421 tacgcgggtt gctgcgcaga caaatatctt cctgcgcggc ctgcgcccat gcaccgtaat
    90481 gcgcagatgt cgcgccaaca tcgcccagtc gtttaccaat cccaacttcc gcaatcagct
    90541 cacttaccgc gttaatagcg tcacgatcgt cggatttttt agttcgcagt gcccgaccaa
    90601 tctgactaaa ccgttcgcga caaaccatcc ggttaaattc catcaccgtt ggcagcaaca
    90661 tggcgttcgc cagaccgtgt ggaatatgca gcgccgcccc tggctgatgc gccatcgcgt
    90721 ggcacaaccc aagccccgca ctggaaaacg ccattcccgc catacatgaa gccagtaaca
    90781 tgctctcgcg cgcggcaagg tcatgaccgt agcccaccgc tttcggcagc gatttgccaa
    90841 tcatcgcaat ggcaccaatc gccagactgt cggtaaacgg tgtagcgttc agggcgctgt
    90901 atgcttcaat ggcatgggtt aacgcatcaa tgccggtcat cgccgtgaca tgcgacggca
    90961 caccttcggt caatgcggcg tcgagaatcg ccacatccgg catcagcgag gcatgggcta
    91021 acacctgctt acgcccgctc accgcatcga taatcaccgt tacgttggtg gtttcagagc
    91081 cggttccggc ggtagtggga atggcaatca acggcaggcg cgattgcaga acgctggttt
    91141 ctgacatctc tgccagcgtg ctatccgggt tcgtcaccag caacgccacg gctttcgccg
    91201 catccagcac cgagccgccg ccaaatgcga tcaccccatc acagcctgac tcacgcaact
    91261 gcgccacggc tgcacacacg tcggtgatgc acggttcgcc caccggacat ggccagagcg
    91321 tcatggcgat acctttaacg gccaggctac gcgtcagccc ggcggtcatc cctgcctgat
    91381 gcaaaaagct gtctgccatc acgaacagat gtttcagccc acgcgtttgc gcttgctgtc
    91441 cgcaactgct caccgagccc ggaccgcaaa gcgtcaccgg tggaacgcta aatgttttta
    91501 cccgttgcag attcagggta tcgaacgcct gaaagagcgc ggtctgcaat tcattttgca
    91561 tatagcccct ccgctttctc tcgcccacta ctggcgatcg ccagcggggt cataaacaaa
    91621 ctgtgctgcg gtaaatgcac ctgtaacgcc gggaattgtt tgcgaaacag ttccgccacg
    91681 cccggttgca tacaagagcc gcccgccagc cataaatcag taatgccctg cccttcaata
    91741 tgccgggcga caatgtccgc cattttttcg tacaccggtt tcaccgcggg ccaaatctcg
    91801 tcaccatgac cgcgcttgta ctgctctgcc tcttccagcg aaatacgacg atttccggcg
    91861 agagtcagag agatgtgatg tccgccggtg gcttcatccg ccgagtacgt caccttgccc
    91921 tttttcacga tggcaatgcc ggtagtgccg ccgccgatat ccactacacc ggcgttgtcc
    91981 agttgcagca gatccgcgac tgcggttggc tcatccagca catggctcac ttccagcccg
    92041 gcagattcca gcacgttgat ggaaatacgc gggtccgtac ctggaggaaa tgaagtcgcc
    92101 gcatggctaa aacgaagacc gaattgctgc tcaagagtgt cgagatggcg acgaacaatg
    92161 gtgaccgcac cgaagaaatc ccagacgatg ccgtcgcgga ccacatcggc ccagtcgagg
    92221 cataccgcca ccggctgacc gtcgcggtca acaaccatcg acaccacatc gcaggtgccc
    92281 agatccaccc ccagccacag cggagattcc gtcgcggcag gcgtctggtt acacagcgtt
    92341 gccgccgttt gcagacgtgg ggtgagccat tgttcgtcgt gcgccatcgg ttactcctta
    92401 aacaatgcga aacgcatcga ctaatacaca gcgacgcaga cggacaaacg tccgcgcgct
    92461 ggttacccct tcaccggttg gcgtggtgat ggtcatggtg gtccagcctt ccccgcccag
    92521 ccccagcccg gcaatgcacg gtccgttctt aacgaaaatg ctggtatcaa tggcgttcgc
    92581 catctggttc atgttttcga tattgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc
    92641 ctccagtttc accgccagcg caatggcatc cgccacgttg gcgacgcgca cgacgggcag
    92701 caccggcatc atcagttcgg tcacggcaaa cggatgttct gcagtggttt ccacaaacag
    92761 caaacgcgtt tcttgcggca cgttcaggcc gattgccgcc gcgattttcg ccgcatcgcg
    92821 accaacccag tcacggctga cggtgccttt tccgcgctcg tcgatatttt tcagcaacac
    92881 cggttgcagc tgctgcgcct gttctgcggt cagtttcacc gcgtgctggc cttccatcag
    92941 acgcatcagt tcatcggcta cgctatcaac aacaatcagt accttttcgt cggcacaaat
    93001 gatgttgtta tcgaaagaag cgcctttgac gatggactga gcggcacggg cgaggtcggc
    93061 ggtttcatcc accactaccg gcgggttgcc agcgcctgcg gcaatcagac gtttattggt
    93121 gtgtttacgc gccgcttcta ctaccgcttc gccgccggtt actaccagca ggccgatacc
    93181 cggaaacttg aacaagcgtt gcgcggtttc gatatccgga tttgccacag taaccagtaa
    93241 gttttccggc ccacctgcgg caacaatcgc ctggttgagc agcgtaatcg cccgctggga
    93301 gacttttttc gccgccggat gcggggcaaa aatgacgctg ttgcccgcgg caatcaggct
    93361 gatggcgttg ttaattacgg ttgccgccgg gttagtggaa ggcgtcaccg aagccaccac
    93421 gccccagggt gcgttttcaa ttagggtcag gccgttgtcg ccagtcagca cttgcggaga
    93481 gaggcactca acgcctggtg tgccgcgcgc ctgagcgacg ttttttgcaa atttatcttc
    93541 aacgcgcccc atgccggttt cactgacggc aagttccgct aaatctctgg cgtgtttttc
    93601 gcctgcttca cgaatggcag caatggctaa ctggcgcatt gccacgcttt ttaacccttg
    93661 ctgggcgact ttggctgccg caacggcgtc atccagggac gcgaaaacgc ccatctcatg
    93721 aacggcggcg gacggcgtgt cactgctttg cattttcagc agtaccgctt tcaccacctg
    93781 ttcaatatcc tgttgattca tgatgttctg ccttatttgt ggaaaattac ctgaccgcca
    93841 gacaccacct catcgacaat gccaatcacg cacagatcga ccggtgacgt ttcgctttta
    93901 tgcgcctggc gggcggaact gccactcacc agtaacaccc actccccggt tcccgcgcca
    93961 atattgtcga tggcgacggc gcattgcccg tcaggattac cttgtggatc aatcatttcc
    94021 accatcagca atttgtcatg cgccagtccg tgatggcgta cggtacaaac aatttgtcca
    94081 gtgacgactg ccagtttcat acccgcctcc gtggcgtatt tcaggtaaaa gctcccccta
    94141 ccctccgcag aaggtaaaat gaaaaaggag agagcgtgac gcccgaatcg acgtcacaca
    94201 gggtgattac aggttgctgc tatcgccttt caggccgatc gggaagactt cttccaggtc
    94261 gccgtgcgga cgcgggataa cgtgtacaga taccagctcg ccgatacgct gtgccgctgc
    94321 ggcacctgca tcggtcgcgg ctttacaggc tgcaacatca ccacgaacca tggcagtaca
    94381 caggccgccg ccaatctgct taacaccaac cagcttgacg cgcgcagctt tcaccatcgc
    94441 gtcagaagcc tcaatcagtg caaccaggcc ccgggtttcg atcattccta atgcttccat
    94501 tgtgctttcc tctttatcag ggtccagaac gggaccgttc attcaaccag tgtttgtaaa
    94561 ctgctttcgc ggttcacttc tgtctgacgc ggcacagctg ccaccagcgc cagctcgata
    94621 atttcctgca cgctacaacc acgagagaga tcgtgcatcg gcgcggcaag tccttgtatc
    94681 agtggcccga cggcacgata tccgccgagt cgttgtgcga ttttgtaacc aatatttccg
    94741 gcttccagcg acggaaaaac catcacattg gccttgccct gtagcgggct ggcaggcgct
    94801 ttttgcgccg ccacttccgg cacgaaggcg gcgtcaaact gtaactcgcc atccaccacc
    94861 agctttggtg cgcgctcacg gacgatttct gtcgcctgct ggacgttagc aacacagggg
    94921 tgacgggcgc taccattgct ggaaaacgac agcatcgcca cgcgcggctc ttctccggtg
    94981 atggcgcgcc aggtttcggc actggcaagc gcgatatccg ccagctgcgc cgccgtcggc
    95041 tgtggcacca cgctgcaatc ggcaaagccc aacgccgggc cgctgtactg tggcaacatc
    95101 aggaaaatgg aagagagcgt tttacagcct ggctgcaagc cgataatgcg taatccggca
    95161 cgcagcacat ttgccgtgga agagaggttg cccgcgatac agacatccgc tttaccggcg
    95221 ctgaccattg ctgcggcgaa catcagaggg tcggtaagtt tttccagcgc atccggcggc
    95281 gttttttcgc ccgcgcgggc cagccagcga tgagcaaatt cttcccgcat tgcgaggttg
    95341 ccatgcggat caatcacctg tagcccgtcc atcgccacgc cgtgactgag cgcaaactga
    95401 cgaagttcaa acggattggc gaccagaatg ggcgttgcca gaccttgttg atgtaaatat
    95461 tgcgcggctt tcagcacacg ttggtctaac gcatccggaa aaaccactct ggcgggcgct
    95521 cgcaacgcca gttcacgaca acgttcaata atcattgcgt ttcccccagt cgttgttgga
    95581 tctgcttcac cgttagcggc tgcttcgtta cgctcaggat catcatcacg tagaccgtgc
    95641 tggaaaggcg gttcagcgcc tgcaaaatgt ccgggcgtaa tacttcaaag ctgcgggtga
    95701 taaacacctg cgccgctacg gtttcggttt cccgcacttt ggtacgtagc agattgagca
    95761 acgccgcatc gcgcccgtgg ctggcttccg gcaccagatg atcgtggtcg agatagcgca
    95821 gcggctggtg agaaagtcga tgcagatctt cgtcagaaag cccgacaatc gcctggcagc
    95881 ccagcggttc acccagcgca tcggcgcgca taatgttgcc aagacgcgaa cggatatccg
    95941 ccagccacgg ctgccacggt tccgccagtt caatttgcag ccacactgcc agcgcaatgg
    96001 tgctgtcgag aaccgcgcga aaacccagac gcggatcgct tttggcgacc attttttccg
    96061 ccgacaggtg agtcagcgta tccggctttt ttgccaccgg ctggcggcac agttcgcagc
    96121 acgcctgcgg atgttcatcg ctactggtca gcccatgaac gggctgcggc tgctgctgtt
    96181 catcgtcaac aaacaggcgg ccctgctcgt caataaactt gatgcgcaga tggcggcttt
    96241 ccagtaactc ccgggcagag ggcgtcaggc gactgtccgc agggagatgg atctctgcgc
    96301 cttcgctgag cgtatggttc gctcttagcc atgcttcggt gatgaaatct ttcataggga
    96361 ttgccagtta gccggccagg cgacatacag gaatttcacg ctggatgtcg taccaaattc
    96421 gatactggag cctttcggga taaacatcac gtcacccgct ttggcgatca tggtttcgcc
    96481 ttcgtggcga acatgcagct ccccttccag caccatgtcg atttcgtcgt agttcagggt
    96541 ccacgggaag aatgcgttct cccactgcat aaaaccagcg gccatgctgc tgccgtcgtc
    96601 tccggtcaca agatcagtca agcccacgca atgcggctct gcgccgtcga aacgaccaaa
    96661 cttgacgctg ctgccatcaa tcactttgat gccgcctttg ccggtcaccg atttgaagct
    96721 cggctgcatt gcgccctgct ccagcgactg tttttccttc atcacttttt ccatcagctg
    96781 tgcgaccagg ctttcggtaa actggccttc cggcagctgg gcaatgatgg tttcgcggat
    96841 gcgctggctt tcggttttgt cggctggcac gctggcgggc acggacgccg ttaccgggat
    96901 cgattcatca cattcggtga tggtaaaacc cagtagatcc gccacttcac gggcttccgg
    96961 ggtaataatg ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg cgtgtgcttc
    97021 acgaatatca ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg gtgagagatg
    97081 ccagataatc caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc tcgaaaatcg
    97141 gctcttcgaa acccgtctca agcaataact ttcgcgttgc ggcgacgtca gcatctggca
    97201 tgtccgtttt actgatgacg gcgatttgcc gtttgctgac gccaatatcc agcaacccgg
    97261 caggcaaccg actttccggg tcatttgcgc catggacata gatcagcatg tctacatcct
    97321 gtagcgtggt aattaaggcg tgataccagc ggggatggct aaaatattca cccggcgtat
    97381 caatatcgcc cttatcatta aattccacgg cctgtgtttt tctggcgagg gtataattcc
    97441 cctgcaacgc attaaataac gttgtttttc ctgccccgac ggtacccaca aacgcaattc
    97501 gtttcatgaa tgcctcgtat taacttttgg tcatttcaca aagcgtataa tttaataagc
    97561 gacctaaacc gctgacggtt tgtgataacg cttcttctac tgcgcccacc gagccataaa
    97621 tcaccaatgc accgctgaag cggtcgagaa aaccgatatg cacatcagcg gctttcagcg
    97681 ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag cgtcataatg ccaattgcgc
    97741 ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg atgagatgcg
    97801 ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta tccatgagtc
    97861 gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa ataacaaaat
    97921 tcggcaagcg cgtttaaaag gtgaggtaga tcactaagaa tggagagata ttcgctgtgg
    97981 tgcataaact ttcatatgca acgcatgaat
//
LOCUS       DAZUFB010000004        95826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZUFB010000004 DAZUFB010000000
VERSION     DAZUFB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.004"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            299..1597
                     /gene="kgtP"
                     /locus_tag="ACHY3M_000305"
     CDS             299..1597
                     /gene="kgtP"
                     /locus_tag="ACHY3M_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFS4315290.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHY3M_000306"
     CDS             complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHY3M_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFS4315291.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHY3M_000307"
     CDS             complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHY3M_000307"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFS4315292.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHY3M_000308"
     CDS             complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHY3M_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFS4315293.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHY3M_000309"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHY3M_000309"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFS4315294.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHY3M_000310"
     CDS             complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHY3M_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFS4315295.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHY3M_000311"
     CDS             7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHY3M_000311"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS4315296.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHY3M_000312"
     CDS             complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHY3M_000312"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4315297.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9595..9978
                     /gene="grcA"
                     /locus_tag="ACHY3M_000313"
     CDS             9595..9978
                     /gene="grcA"
                     /locus_tag="ACHY3M_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFS4315298.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10034..10621)
                     /gene="eamB"
                     /locus_tag="ACHY3M_000314"
     CDS             complement(10034..10621)
                     /gene="eamB"
                     /locus_tag="ACHY3M_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFS4315299.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHY3M_000315"
     CDS             10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHY3M_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4315300.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11816..13150)
                     /gene="srmB"
                     /locus_tag="ACHY3M_000316"
     CDS             complement(11816..13150)
                     /gene="srmB"
                     /locus_tag="ACHY3M_000316"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFS4315301.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13282..14019
                     /gene="trmN"
                     /locus_tag="ACHY3M_000317"
     CDS             13282..14019
                     /gene="trmN"
                     /locus_tag="ACHY3M_000317"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFS4315302.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14004..15626)
                     /gene="nadB"
                     /locus_tag="ACHY3M_000318"
     CDS             complement(14004..15626)
                     /gene="nadB"
                     /locus_tag="ACHY3M_000318"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFS4315303.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15882..16037
                     /gene="rseD"
                     /locus_tag="ACHY3M_000319"
     CDS             15882..16037
                     /gene="rseD"
                     /locus_tag="ACHY3M_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFS4315304.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16034..16609
                     /gene="rpoE"
                     /locus_tag="ACHY3M_000320"
     CDS             16034..16609
                     /gene="rpoE"
                     /locus_tag="ACHY3M_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFS4315305.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16642..17292
                     /gene="rseA"
                     /locus_tag="ACHY3M_000321"
     CDS             16642..17292
                     /gene="rseA"
                     /locus_tag="ACHY3M_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFS4315306.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17292..18248
                     /gene="rseB"
                     /locus_tag="ACHY3M_000322"
     CDS             17292..18248
                     /gene="rseB"
                     /locus_tag="ACHY3M_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFS4315307.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18245..18724
                     /gene="rseC"
                     /locus_tag="ACHY3M_000323"
     CDS             18245..18724
                     /gene="rseC"
                     /locus_tag="ACHY3M_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFS4315308.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18922..20721
                     /gene="lepA"
                     /locus_tag="ACHY3M_000324"
     CDS             18922..20721
                     /gene="lepA"
                     /locus_tag="ACHY3M_000324"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4315309.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20737..21711
                     /gene="lepB"
                     /locus_tag="ACHY3M_000325"
     CDS             20737..21711
                     /gene="lepB"
                     /locus_tag="ACHY3M_000325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4315310.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21984..22664
                     /gene="rnc"
                     /locus_tag="ACHY3M_000326"
     CDS             21984..22664
                     /gene="rnc"
                     /locus_tag="ACHY3M_000326"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4315311.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22661..23566
                     /gene="era"
                     /locus_tag="ACHY3M_000327"
     CDS             22661..23566
                     /gene="era"
                     /locus_tag="ACHY3M_000327"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4315312.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23578..24306
                     /gene="recO"
                     /locus_tag="ACHY3M_000328"
     CDS             23578..24306
                     /gene="recO"
                     /locus_tag="ACHY3M_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4315313.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTCMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24318..25049
                     /gene="pdxJ"
                     /locus_tag="ACHY3M_000329"
     CDS             24318..25049
                     /gene="pdxJ"
                     /locus_tag="ACHY3M_000329"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFS4315314.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25049..25429
                     /gene="acpS"
                     /locus_tag="ACHY3M_000330"
     CDS             25049..25429
                     /gene="acpS"
                     /locus_tag="ACHY3M_000330"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4315315.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25849..25929
                     /gene="shoB"
                     /locus_tag="ACHY3M_000331"
     CDS             25849..25929
                     /gene="shoB"
                     /locus_tag="ACHY3M_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFS4315316.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26123..26383)
                     /gene="yfhL"
                     /locus_tag="ACHY3M_000332"
     CDS             complement(26123..26383)
                     /gene="yfhL"
                     /locus_tag="ACHY3M_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFS4315317.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26439..27287)
                     /gene="yfhH"
                     /locus_tag="ACHY3M_000333"
     CDS             complement(26439..27287)
                     /gene="yfhH"
                     /locus_tag="ACHY3M_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4315318.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27496..28131
                     /gene="yfhb"
                     /locus_tag="ACHY3M_000334"
     CDS             27496..28131
                     /gene="yfhb"
                     /locus_tag="ACHY3M_000334"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFS4315319.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28189..28692
                     /gene="tadA"
                     /locus_tag="ACHY3M_000335"
     CDS             28189..28692
                     /gene="tadA"
                     /locus_tag="ACHY3M_000335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4315320.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(28689..30245)
                     /gene="mltF"
                     /locus_tag="ACHY3M_000336"
     CDS             complement(28689..30245)
                     /gene="mltF"
                     /locus_tag="ACHY3M_000336"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFS4315321.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30503..34390
                     /gene="purL"
                     /locus_tag="ACHY3M_000337"
                     /gene_synonym="purI"
     CDS             30503..34390
                     /gene="purL"
                     /locus_tag="ACHY3M_000337"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFS4315322.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELYLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34966..36393
                     /gene="qseE"
                     /locus_tag="ACHY3M_000338"
     CDS             34966..36393
                     /gene="qseE"
                     /locus_tag="ACHY3M_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFS4315323.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36558..37271
                     /gene="qseG"
                     /locus_tag="ACHY3M_000339"
     CDS             36558..37271
                     /gene="qseG"
                     /locus_tag="ACHY3M_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFS4315324.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            37261..38595
                     /gene="glrR"
                     /locus_tag="ACHY3M_000340"
     CDS             37261..38595
                     /gene="glrR"
                     /locus_tag="ACHY3M_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFS4315325.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38656..38994
                     /gene="glnB"
                     /locus_tag="ACHY3M_000341"
     CDS             38656..38994
                     /gene="glnB"
                     /locus_tag="ACHY3M_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFS4315326.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(39039..40229)
                     /gene="hmpA"
                     /locus_tag="ACHY3M_000342"
     CDS             complement(39039..40229)
                     /gene="hmpA"
                     /locus_tag="ACHY3M_000342"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFS4315327.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40557..41810
                     /gene="glyA"
                     /locus_tag="ACHY3M_000343"
     CDS             40557..41810
                     /gene="glyA"
                     /locus_tag="ACHY3M_000343"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4315328.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41874..43067)
                     /gene="yphH"
                     /locus_tag="ACHY3M_000344"
     CDS             complement(41874..43067)
                     /gene="yphH"
                     /locus_tag="ACHY3M_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4315329.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43185..46466
                     /gene="yphG"
                     /locus_tag="ACHY3M_000345"
                     /pseudo
     CDS             43185..46466
                     /gene="yphG"
                     /locus_tag="ACHY3M_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            46563..47546
                     /gene="yphF"
                     /locus_tag="ACHY3M_000346"
                     /pseudo
     CDS             46563..47546
                     /gene="yphF"
                     /locus_tag="ACHY3M_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            47569..49080
                     /gene="yphE"
                     /locus_tag="ACHY3M_000347"
     CDS             47569..49080
                     /gene="yphE"
                     /locus_tag="ACHY3M_000347"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS4315330.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQVIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            49105..50103
                     /gene="yphD"
                     /locus_tag="ACHY3M_000348"
     CDS             49105..50103
                     /gene="yphD"
                     /locus_tag="ACHY3M_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315331.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            50136..51230
                     /gene="yphC"
                     /locus_tag="ACHY3M_000349"
     CDS             50136..51230
                     /gene="yphC"
                     /locus_tag="ACHY3M_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS4315332.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            51242..52114
                     /gene="yphB"
                     /locus_tag="ACHY3M_000350"
     CDS             51242..52114
                     /gene="yphB"
                     /locus_tag="ACHY3M_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFS4315333.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(52162..52584)
                     /gene="yphA"
                     /locus_tag="ACHY3M_000351"
     CDS             complement(52162..52584)
                     /gene="yphA"
                     /locus_tag="ACHY3M_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS4315334.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(52681..53883)
                     /gene="hcaD"
                     /locus_tag="ACHY3M_000352"
     CDS             complement(52681..53883)
                     /gene="hcaD"
                     /locus_tag="ACHY3M_000352"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFS4315335.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(53893..54705)
                     /gene="hcaB"
                     /locus_tag="ACHY3M_000353"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(53893..54705)
                     /gene="hcaB"
                     /locus_tag="ACHY3M_000353"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFS4315336.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(54702..55022)
                     /gene="hcaC"
                     /locus_tag="ACHY3M_000354"
     CDS             complement(54702..55022)
                     /gene="hcaC"
                     /locus_tag="ACHY3M_000354"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFS4315337.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(55022..55540)
                     /gene="hcaF"
                     /locus_tag="ACHY3M_000355"
     CDS             complement(55022..55540)
                     /gene="hcaF"
                     /locus_tag="ACHY3M_000355"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFS4315338.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(55537..56898)
                     /gene="hcaE"
                     /locus_tag="ACHY3M_000356"
     CDS             complement(55537..56898)
                     /gene="hcaE"
                     /locus_tag="ACHY3M_000356"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFS4315339.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            57034..57924
                     /gene="hcaR"
                     /locus_tag="ACHY3M_000357"
     CDS             57034..57924
                     /gene="hcaR"
                     /locus_tag="ACHY3M_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFS4315340.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            58084..59223
                     /gene="hcaT"
                     /locus_tag="ACHY3M_000358"
     CDS             58084..59223
                     /gene="hcaT"
                     /locus_tag="ACHY3M_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFS4315341.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(59215..60495)
                     /gene="csiE"
                     /locus_tag="ACHY3M_000359"
     CDS             complement(59215..60495)
                     /gene="csiE"
                     /locus_tag="ACHY3M_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFS4315342.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(60686..61540)
                     /gene="yfhR"
                     /locus_tag="ACHY3M_000360"
     CDS             complement(60686..61540)
                     /gene="yfhR"
                     /locus_tag="ACHY3M_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4315343.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(61685..62488)
                     /gene="suhB"
                     /locus_tag="ACHY3M_000361"
     CDS             complement(61685..62488)
                     /gene="suhB"
                     /locus_tag="ACHY3M_000361"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFS4315344.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            62607..63344
                     /gene="trmJ"
                     /locus_tag="ACHY3M_000362"
     CDS             62607..63344
                     /gene="trmJ"
                     /locus_tag="ACHY3M_000362"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFS4315345.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFSR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            63645..64133
                     /gene="iscR"
                     /locus_tag="ACHY3M_000363"
     CDS             63645..64133
                     /gene="iscR"
                     /locus_tag="ACHY3M_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFS4315346.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            64374..65588
                     /gene="iscS"
                     /locus_tag="ACHY3M_000364"
     CDS             64374..65588
                     /gene="iscS"
                     /locus_tag="ACHY3M_000364"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4315347.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            65616..66002
                     /gene="iscU"
                     /locus_tag="ACHY3M_000365"
     CDS             65616..66002
                     /gene="iscU"
                     /locus_tag="ACHY3M_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFS4315348.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            66019..66342
                     /gene="iscA"
                     /locus_tag="ACHY3M_000366"
     CDS             66019..66342
                     /gene="iscA"
                     /locus_tag="ACHY3M_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFS4315349.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            66438..66953
                     /gene="hscB"
                     /locus_tag="ACHY3M_000367"
     CDS             66438..66953
                     /gene="hscB"
                     /locus_tag="ACHY3M_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFS4315350.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            66970..68820
                     /gene="hscA"
                     /locus_tag="ACHY3M_000368"
     CDS             66970..68820
                     /gene="hscA"
                     /locus_tag="ACHY3M_000368"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFS4315351.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            68822..69157
                     /gene="fdx"
                     /locus_tag="ACHY3M_000369"
     CDS             68822..69157
                     /gene="fdx"
                     /locus_tag="ACHY3M_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFS4315352.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            69169..69369
                     /gene="iscX"
                     /locus_tag="ACHY3M_000370"
     CDS             69169..69369
                     /gene="iscX"
                     /locus_tag="ACHY3M_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFS4315353.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            69547..70830
                     /gene="pepB"
                     /locus_tag="ACHY3M_000371"
     CDS             69547..70830
                     /gene="pepB"
                     /locus_tag="ACHY3M_000371"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFS4315354.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            70972..71748
                     /gene="sseB"
                     /locus_tag="ACHY3M_000372"
     CDS             70972..71748
                     /gene="sseB"
                     /locus_tag="ACHY3M_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFS4315355.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(71780..71905)
                     /gene="timP"
                     /locus_tag="ACHY3M_000373"
     CDS             complement(71780..71905)
                     /gene="timP"
                     /locus_tag="ACHY3M_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFS4315356.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(72241..73086)
                     /gene="sseA"
                     /locus_tag="ACHY3M_000374"
     CDS             complement(72241..73086)
                     /gene="sseA"
                     /locus_tag="ACHY3M_000374"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFS4315357.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            73293..78254
                     /gene="yfhM"
                     /locus_tag="ACHY3M_000375"
     CDS             73293..78254
                     /gene="yfhM"
                     /locus_tag="ACHY3M_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFS4315358.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTG
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMVLNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            78255..80567
                     /gene="pbpC"
                     /locus_tag="ACHY3M_000376"
     CDS             78255..80567
                     /gene="pbpC"
                     /locus_tag="ACHY3M_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFS4315359.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            80716..81147
                     /gene="ndk"
                     /locus_tag="ACHY3M_000377"
     CDS             80716..81147
                     /gene="ndk"
                     /locus_tag="ACHY3M_000377"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4315360.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            81297..82451
                     /gene="rlmN"
                     /locus_tag="ACHY3M_000378"
     CDS             81297..82451
                     /gene="rlmN"
                     /locus_tag="ACHY3M_000378"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFS4315361.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRDKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            82736..83749
                     /gene="rodZ"
                     /locus_tag="ACHY3M_000379"
     CDS             82736..83749
                     /gene="rodZ"
                     /locus_tag="ACHY3M_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFS4315362.1"
                     /translation="MNTEATHDQNEALTTGARLHNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            83776..84894
                     /gene="ispG"
                     /locus_tag="ACHY3M_000380"
                     /gene_synonym="gcpE"
     CDS             83776..84894
                     /gene="ispG"
                     /locus_tag="ACHY3M_000380"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFS4315363.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            85005..86279
                     /gene="hisS"
                     /locus_tag="ACHY3M_000381"
     CDS             85005..86279
                     /gene="hisS"
                     /locus_tag="ACHY3M_000381"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4315364.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            86297..86917
                     /gene="yfgM"
                     /locus_tag="ACHY3M_000382"
     CDS             86297..86917
                     /gene="yfgM"
                     /locus_tag="ACHY3M_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFS4315365.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            86928..88106
                     /gene="bamB"
                     /locus_tag="ACHY3M_000383"
     CDS             86928..88106
                     /gene="bamB"
                     /locus_tag="ACHY3M_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFS4315366.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            88224..89696
                     /gene="der"
                     /locus_tag="ACHY3M_000384"
     CDS             88224..89696
                     /gene="der"
                     /locus_tag="ACHY3M_000384"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4315367.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            89765..89980
                     /gene="yfgJ"
                     /locus_tag="ACHY3M_000385"
     CDS             89765..89980
                     /gene="yfgJ"
                     /locus_tag="ACHY3M_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS4315368.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(89977..91347)
                     /gene="xseA"
                     /locus_tag="ACHY3M_000386"
     CDS             complement(89977..91347)
                     /gene="xseA"
                     /locus_tag="ACHY3M_000386"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4315369.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRLFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            91509..92975
                     /gene="guaB"
                     /locus_tag="ACHY3M_000387"
     CDS             91509..92975
                     /gene="guaB"
                     /locus_tag="ACHY3M_000387"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4315370.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            93044..94621
                     /gene="guaA"
                     /locus_tag="ACHY3M_000388"
     CDS             93044..94621
                     /gene="guaA"
                     /locus_tag="ACHY3M_000388"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4315371.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(94716..95255)
                     /gene="yfgI"
                     /locus_tag="ACHY3M_000389"
     CDS             complement(94716..95255)
                     /gene="yfgI"
                     /locus_tag="ACHY3M_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFS4315372.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(95271..95789)
                     /gene="yfgH"
                     /locus_tag="ACHY3M_000390"
     CDS             complement(95271..95789)
                     /gene="yfgH"
                     /locus_tag="ACHY3M_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS4315373.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT        22761 a        24094 c        26116 g        22855 t
ORIGIN      
        1 gtcaggatgg cctttttgcg ttggtgaaaa aaatgcctga tgcgacgctg cgcgtcttat
       61 actcccacat atgccagatt cagcagcgaa tacggcttcc ccaacttgcc cacctccata
      121 cgtgttctcc tcaccagaaa tttatcctta agctcctcaa taaccatttt cctgctaact
      181 aaattcatgg ttaaggttgc ataatgatac gcaacaaatg tataacattt tctttaccaa
      241 aagaaataaa caaaagcgac cgacaaaagc atcggattac ggcaggagac ataatggcat
      301 ggctgaaagt actgtaacgg cagacagcaa actgacaagt agtgatacac gtcgccgcat
      361 ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag tggttcgatt tctatgtcta
      421 ctcgttctgt tcactctact tcgcccacat tttcttccct tccgggaaca cgacgactca
      481 actactacaa acagcaggtg tttttgctgc gggattcctg atgcgcccaa taggcggttg
      541 gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa tcgatgctgt tatcggtgtg
      601 tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca ggttatgaaa ctataggtac
      661 gtgggctccg gcattattgc ttctcgctcg tttatttcag ggattatccg ttggcggaga
      721 atatggcacc agcgccacct atatgagtga agttgccgtt gaagggcgca aaggttttta
      781 cgcatcattt cagtatgtga cgttgatcgg cggacaactg ctagccctac tggttgtcgt
      841 ggttttacaa cacaccatgg aagacgctgc actcagagag tggggatggc gtattccttt
      901 cgcgttagga gctgtgttag ctgttgtggc gttgtggtta cgtcgtcaat tagatgaaac
      961 ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct ctgaaaggat tatggcgcaa
     1021 tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg ggctcccttt gtttctatac
     1081 cttcaccact tatatgcaga agtatctggt aaatactgcg ggaatgcatg ccaacgtggc
     1141 gagtggcatt atgactgccg cattgtttgt attcatgctt attcaaccac tcattggcgc
     1201 gctgtcggat aagattggtc gccgtacctc aatgttatgt ttcggttcgc tggcagccat
     1261 ttttaccgtt cctattctct cagcattgca aaacgtttcc tcgccttatg ccgcttttgg
     1321 tctggtgatg tgtgccctgc tgatagtgag tttttataca tcaatcagtg gaatactgaa
     1381 ggctgagatg ttcccggcac aggttcgcgc attaggcgtt ggcctgtcat atgcggtcgc
     1441 taatgctata tttggtggtt cggcggagta cgtagcgttg tcgctgaaat caataggaat
     1501 ggaaacagcc ttcttctggt atgtgacctt gatggccgtg gtggcgtttc tggtttcttt
     1561 gatgctacat cgcaaaggga aggggatgcg tctttagtga cgggtcagtt gccagacggt
     1621 atagccggtg cttgcaccgg cgacatccca ggccaaatcc ttccagctcc agccgctccc
     1681 ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa ctgacagaga acatcaatcc
     1741 aaacatggca ctgcgatccc ggctcatccc ctgatgctgt gaatattcat ttccggcggc
     1801 ggaaagcatc gccgaggcga taaagtgttg agctttatcc tgcccgctcc agctatcgtt
     1861 agccatatgg ctgcatccag aaagtaacaa tagcgaacag acaaaaagaa tacgcaaaac
     1921 gtggcctcca aaaaacaaac cccgtacagg acggggttgt gattacagga tgcggctaat
     1981 taatcggtcg atgcggatac ggcgcaaacg gcggatgagt ttacgaacct tcaccggata
     2041 atcggcaata ctttgcagat cgcgatagtg cttaacgatg gtggtatgct cgcggatcag
     2101 ctccagttct ttctctcgct gcggcgcaag ctccagttgt ggatcgtgga tcaaaatggc
     2161 gttttccaga tccagacgcc aggcgcgcgg gttcaggtta ttaccggtga tcaacatcca
     2221 cttatcatca acccacatcc cttttaggtg ataggtgttg tcgtcatctt tccataaccg
     2281 aaccactagc tggtcagtat tgacgtaata ctgcaaacgg ctcaggaaac gacgcagatt
     2341 gatctcatag agataaggca atgcgccaat tatcttgaaa ggttcatctt ccggaatgta
     2401 gaagtcgttc gcggttttat caccaacaat aatttcgacc tttttccctt cgcgcagcaa
     2461 ctggataata ttgcgcacaa ggattgctgg caggttgaag tatggcgtac agatggttag
     2521 tttctgctcg gcacaaggca taagatggaa aatggtcttg ttcaacagac tcgatttccc
     2581 cagccccact agcggcgtta cggaaagctg atcgttgtcg gcatcgccct ggaaatgata
     2641 agcggcatca cgcagctcct ggcggaacag acgaatatcg ttcttgattt ccgggctttt
     2701 tggccgatta acatcatcca gacgattaac gccgcggcca ttcataatat tctgtgtaac
     2761 ccattcaaac ataatgtctg acatcttacg gttacggata agatgataac ggtcgtagcg
     2821 atatttatcg tgctgatgca ggtaaacatc gttcaggctg gcaccgctat aaagtacgct
     2881 atcgtcgatg ataaagcctt taaagtgcag aacaccaagg gcttcacggg tattgattgg
     2941 aacgccataa accggaacat ctacgcccgg attttcctgc gccatgcggc agtaccagtc
     3001 agcgttagtg ttagatgccg cagcgccaat gcgtccacgt tgtgcacgat gccagtcgac
     3061 cagcacccgc acatccagtt ccggacgctg ccttttagct tcatacaacg cgttcagaat
     3121 gcctttgcca ccgtcatcct gttcgagata cagggcgaca atgcaaatgc gctgcttcgc
     3181 gctggctatt ttttccagca gcgtctcccg gaagtcggcg ggagcgtaaa agaaatcgac
     3241 atcatcaact gattgagaaa tcttgggtag ttgggcaagg tgttgttgat gtttattacg
     3301 cttaaatttt gacaacatca cagtgcattt cttctctgtt cattgaaggg tcctctgtgc
     3361 aatgcagacg acataagcgg gcaataataa caccagtccc gattaagtgg tcaacatttc
     3421 cagtacctta ctcatgattc ctcgcgttgg gcaagattta gcgtaagccc aacgatcccc
     3481 tcttcgagct ggatatcaac gttaaacccg agcttgcggg ctagcgccac catgccacga
     3541 ttgtttggca tcgtaatacc attcagacgt tgtagtccgt gatctcgcgt ataggtaatc
     3601 aacttttcca ttaagcgtcg acctaagcct aaccctttga gatccgagcg aaccagcaca
     3661 gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac gcgtgacgcc gaggatctct
     3721 tccgtttgat caatacgtcg taccgctaca aacgccattt cccgatcgta gtcgatctgt
     3781 gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct cgctaaagta gcggtaataa
     3841 agatcttctt tggtgactcg cgaaatgaat tgctgaagtt gtggctcatc ttctggcaaa
     3901 atcgggcgga acaagcagcg ttcaccgttt ttcaattcta cccattcttc cagctgatgc
     3961 ggataagggc gcactgccag ccgactctcg ttatcgcctt caaacggcga gatatccagc
     4021 gtgacatcca gcgcggtaaa ttcactgcca gaagccagca aaggatgaat atccagacgc
     4081 tgaatttccg ggcaatcgac aatcaagttg gaaacctgca ccagaagctg gctcaagcct
     4141 gcaacatcca atgggcgtag cgcactgcgt gcacgaatct ttttactttt gatcccctga
     4201 ataaccagat agcgggccag gttcatgttc agcggcggta acgcgacgac ggcttgatct
     4261 tcaggacgcc actccacacc gccttcgccc agcatgatca acggcccgaa aaccggatcg
     4321 tgctcaacca caacccgcaa ctcctgagca ccagcacggt tagccatact ttgcaccaac
     4381 aggccgtgga cccgtgcctg cggccaggcc atttttacgc gatcgaaaat ggcgtttgcc
     4441 gcttgctgga cttcattggc tgtacgcagg tagagcatga cgccctgaac ttccgattta
     4501 tgtggaatat ccggcgaacg caatttcagc gccaccggat aaccaatctg ttcggcaata
     4561 tgcaccgctt cggtgctatc gctggcaatc caggtgggga gcgtgttcat gccatatgct
     4621 tgcaggatag gctgaacttc atgggtatcg agtgacgttg ctccttcggc aatcgcctgt
     4681 tgcaacagaa gatgcgcttc tgcggtattg gaggtcagat tgctgggcaa cgccggcgtt
     4741 tcgcgtagtt gcttctgatt acgccggtac tccaccatat gcataaaagc agtgatggtt
     4801 ccttccgggg tacggtaggt cggcagcccg gcttcgctga ataatcgtcg tgcctcttgc
     4861 gaggaatgtt cgccacacca gttcgtcagc agagaaacat acttgctgcg gggatgatgc
     4921 tttaccgctt caattaatac ttgcgcgctt tctgttgcgg gagcagcggc gctgggcgaa
     4981 tgaataacca tcagcgcatc aaaatcctgg ctgtggagca gaatgtccag cgttttaata
     5041 tagtgctcac tgctggcgtc atcgcgcaga tcgagcgggt tagagattgc cacatgtcct
     5101 ggcagtgcat cgcgcagttt ctggcaggtt tcttcgctta gtgttgccag cttgccattg
     5161 cgtgaccata aggcatcaag cgccagcgcg gcaggcgcag caccgttgct gataatcatc
     5221 aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt ccaccgccga aaacagttcg
     5281 tgggtgtcct gtacccgcaa caaaccggca cgctgaatag ccgcatccca cgccggatcc
     5341 attcctgccg tggtgttcaa cagacgttga gccgccgggc tacgtccgct tttaatcacc
     5401 agaatcggtt tgttgcgaga ggcactacgg gccgccgaaa caaagcgtcg cgcgtcgctt
     5461 aactgttcga gatagagcag gatggcgctg gttttactgt cgcgtgccag atagtcaagc
     5521 aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa taaagtagga aaagcccatc
     5581 ttacgctgtt gcgcccagtc gaggatggtg ttggagacgg cagccgattg cgaaataaac
     5641 gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg cattcagacc ttgccaggga
     5701 gccagtaaac ccagactgtt tggtccaagc aggcgcatgt tatggcgcag ggcgcaggcg
     5761 cggagatctt cgtgttgcga tgccggggcg gaaagaataa tgcaggtttt acagcctttc
     5821 tcgccgagct cttccagaag agcaagatta cggctggcat tggtacataa aaccgcaagg
     5881 tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca acacacccaa caccgctttc
     5941 caggctggcg tcaccgggag taccggtccg ttaaagcctc ccgccagcag gttacgcatc
     6001 atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa ttaccgctat cgattttggt
     6061 cgcagtagtg cttccagtcc tcgctgactc ataccggtct cccgcagaag tgaccgaatg
     6121 attttaaacg ctttctaact gttctgctgt gatgctaccc agatgttgcg tttttcctgc
     6181 cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac cctgctgccg ccccggtatc
     6241 gctggccata tctaacagtg cgatggctac ctcggcagta caatattggc ctgcagcctg
     6301 ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg acggaaatga cgggaagatt
     6361 atccagatac ggacttttac gaaacatctt gcgagcttcc ggccaggtac catcgagcat
     6421 gataaacagc ggtggcttac cggcaggtgg tgtgaagatc acttcccgtt gctcatcagc
     6481 atacgaagcg ggaaagacca ccattggctg atagtccggg ttttgcacca actccagcaa
     6541 atcctgcgag ggttcggtac gcgaccattg aaacgcaacg gtatcaggca aaatatcagc
     6601 aatcagacgc ccggtattac tgggcttcat tggctcggtg tcgaacatca gcaaacagaa
     6661 gcgacttttt gcttgtgctg gggtaattgt cgaacagaga cataatttct ctggcaaaag
     6721 acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga aaaggacgtg ttgcgcgcgc
     6781 aatacgctcg gcgcgtaact ggagaacacc gttttcggtc ataagagagc gtcgaaaaaa
     6841 cgccattgtc gcagaggaga aaacggggca caagatgcgc cccggtaaga ttaaagagat
     6901 tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc cattaagcat gtcgacaacc
     6961 tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa ttccaaagcg actgctcaat
     7021 tcacgttcag cttcggtatt cactttcaca aagcgcactt taccgctacg ctcttgcgcg
     7081 acatcttcaa aaattggtgc gaaattacgg caggggccgc accacggtgc ccagaagtcg
     7141 atcaccacag gtagatcatc cttcagcaat ttgtcgagtg tttcaccggt cgcattaatc
     7201 acctctccgt caaacaagtc gtgaccgcag cgtccgcatt ttgccgcatc ttcgatccga
     7261 tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa cggtattcat aactaacctc
     7321 gggatgagta aggagactta tcggcaacga cgccggtatg tttctaatat gttacatatt
     7381 atcggggaca ttgttccaga caacaatctg ttttattcaa taggtatgat agtcgcaggc
     7441 tttggtacga aatcatggcg aaccgcgcgg acttcgggta atctgcgcgc ttcgcgcagc
     7501 gctggtggag aaaagcatga acgatgaaat gaaaggtaaa agcggcaagg tcaaagtgat
     7561 gtatgtccgc agtgatgatg attctgataa acgtacccac aacccgcgta ccgggaaagg
     7621 gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt cgccccgccc gcgatgacaa
     7681 acagagtcag ccccgtgacc gcaagtggga agattcgccg tggcgcacgg tttcccgcgc
     7741 gccgggtgat gagacgccgg aaaaggccga tcacggtggc atcagtggta aaagttttat
     7801 tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc cgcgtctacg gcgaaaatgc
     7861 atgtcaggca cttttccaga gccgtccgga agcgattgtt cgcgcctggt ttatccagag
     7921 tgtaacgccg cgttttaaag aagccttgcg ctggatggca gcaaaccgta aagcgtacca
     7981 tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg gaacatcacg gcggcgtttg
     8041 cttcttgatc aaaaagcgta acggtacaac cgtgcagcag tgggtaagcc aggcaggcgc
     8101 gcaggattgc gttctggcac tggaaaacga atctaacccg cataacctgg gtggcatgat
     8161 gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg caggatgcgg cactgctgga
     8221 gtcgggggcg gctatccgta ccgcagaagg cggcgcagag cacgttcagc cgattactgg
     8281 cgacaacatt gttaacgtgc tggatgattt ccgtcaggca ggttacaccg tagtgaccac
     8341 ttccagcgag cagggtaaac cgctgttcaa aactagtctg ccagcgaaaa tggtactggt
     8401 gctgggtcag gaatatgaag ggttaccgga tgccgcacgc gatccgaacg atctgcgcgt
     8461 gaagattgac ggtacgggca acgttgccgg gctgaatatc tctgtcgcaa ccggcgtttt
     8521 gcttggtgaa tggtggcgtc agaataaagc ctgataaatc agccgggtgg caactctgcc
     8581 atccggcatt tccccgcaaa tttactcact ctctgccggt aatactggca tccagtcaat
     8641 cggcgtctcg ccacgttgtt ccagccactg atttgccagc acaaaatggt tgcagccaaa
     8701 gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct ttcagtacat gatggcgttg
     8761 cttatctata atcgcccctt tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc
     8821 ttcgcggtgc tggttaatca ggctgatgac tttatcagta aacgtctccc aaccgaggct
     8881 ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca gtattgagca gcagaacgcc
     8941 ctgacgcgcc cagctttcga gatagccatg attagggcgg gtgaagcccg gaatagtatt
     9001 ttccagctct ttatacatat tcaataatga cggaggaatg gcaatgccgg gacgaacgga
     9061 aaatgccaga ccatgcgcct gtcccggtcc gtgataagga tcctggccga gaatcaccac
     9121 tttaacgtca cccaactcgg taaagcggaa cgcgttaaag acatcttttt gtggtggata
     9181 gatagtgacg ccggactgcc gctcgctggc gacggtctga agggtattaa gaaaataagg
     9241 ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat tcgttagcca tctcgctctc
     9301 ctgcgaatct tcaatccgcc tagcttaact gcttctttct acagaacaaa atcatgctca
     9361 ttttttagac ttaaacgagt gcaaaaaatg taaattggta aaatttcaca cccgcataag
     9421 ttgatgtaaa acaataaaat ccatttacga ccacaatcga tgtgtggttt ttattgattt
     9481 aaatcaaaga atcaagggtg gttgaggagt atatatacac tcaagcaaca atggttttac
     9541 caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca tcacatgatt
     9601 acaggtatcc agattactaa agccgctaac gacgatctgc tgaactcttt ctggctgctg
     9661 gacagcgaaa aaggcgaagc acgttgcatc gttgcaaaag caggttatgc agaagatgaa
     9721 gtggttgcag taagcaaact gggtgacatt gaataccgtg aagttccagt agaagtgaaa
     9781 ccagaagttc gcgttgaagg tggtcaacac ctgaacgtta acgttctgcg tcgtgaaact
     9841 ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg tgtatccggt
     9901 tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat cgctcgtacc
     9961 tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg gcgacctctt
    10021 ccgctttttt tcgttaatag aaaatgcgta ccgcgcaata gaccagcaac agggcaagca
    10081 cgatatttaa ctggcgacca tactggcgaa acaatcgctg aaacagatgc cccgccagcg
    10141 cccagcacac attgccaaac gtcccaatca tcgccagcaa aacgctgacg ccaactaccc
    10201 agcttagcgc ctgtgtttgc ggcagaacaa acgtcgacag tgccgtaaca ccgtacaaaa
    10261 tgattttgac gttcacaaac tgcaaagcaa agctggccca aaagctgatt ggttttgcct
    10321 gaagtccgtc ttcctttgtt gggctggtgg cgattttcca cgccagccag acaatatatg
    10381 ccgcccccgc ccaactcaaa aggtgtaccg ctgccgggtc aatcactgcc agtgaaaatg
    10441 aaatgcccgc acacagtaac atcacaatca aaaatcccag actcatccct gccagcacgc
    10501 gggtactttg acgaaatcca tgcgacgtag cagagctaag ggcgagaata ttgttcggtc
    10561 ctggcgtcat agcggtaatc agggtgtaag tccaaaaagc acttaaaagg gtcggtgtca
    10621 cttgttatct cctctgtttt ggtagacagt acccgacacg gaacatttaa aaaaaatgaa
    10681 tgtttttata ttctagattc gatattttcg ataggtttgg ggttatggat ctgcgccgtt
    10741 ttattacgct taaaaccgtg gtggaagagg gttccttttt gcgagcttcg caaaaattgt
    10801 gctgtacaca atcgacggtg acttttcata ttcagcagct tgagcaggag ttttcagtcc
    10861 agttatttga gaaaattggc cgacggatgt gccttacccg cgaaggaaaa aagttgctgc
    10921 cgcacattta tgaacttacc cgagtgatgg atacgcttcg tgaggctgcc aaaaaggagt
    10981 cagatccgga cggggaacta cgcgttgttt cgggcgaaac gttgctctct taccgaatgc
    11041 cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt gcgtttatcg ttgcagtcgc
    11101 tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggccgatgtc ggcgtctttt
    11161 atcgcgtagg gaatgatgat gccctgaacc gacgagagtt gggtgaacaa tcgctggtgc
    11221 tggtggcttc accgcaaatt gcagatgtcg attttactga gccgggaaga cataacgcct
    11281 gtagctttat tatcaacgaa ccgcaatgtg tcttccggca gatatttgag agcacgctgc
    11341 gtcagcggcg gatcacggtg gaaaacacca ttgagctgtt aagcattgaa agtatcaaac
    11401 gttgtgtcgc ggcgaatatc ggtgtcagtt acttgccccg ctttgcggtg gaaaaagagc
    11461 ttgagagcgg agaattaatt gaattgcctt ttggcgaaca gtcgcagacc attacggcca
    11521 tgtgcgcaca ccatgctgga aaagcggtta gcccggcgat gcacactttt attcagtgtg
    11581 ttgaagagag ttttgtggct ggataaaaat aatgtcggga ttaaacccgg catttaactt
    11641 gtcgacaaag tgcgctttat tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    11701 gatcgtgcaa attcaatata ttgcatgagc cacgtagata ctgataagcg tagcgcatca
    11761 ggcagttttg cgtttgccag cacttttaat accaggctta aacccggcat tgagattact
    11821 cttctgtcgt ttgcggtggc acgcccgttc cgcttggttt acggcgctta ccaatatttt
    11881 tggtgtcgcg atggcgtttt ttcacacgcg gtttctcttt ctctttagct tttttcttct
    11941 cggcgcgttt tgccagtact ttcttcgatg gcttgccggt ctgcttctcg cttggcgcac
    12001 gcgttttcgg gcgtaactca tcaataacgc gagctttaat cggctcttca atgtaacggc
    12061 caaccttgcc cagcagcaaa tggtcatggg cttccaccag cgaaattgcg gtgcctttac
    12121 gaccggcgcg cgcggtacgt ccgatacggt gcaaataagt atcgccactg cgcggcatat
    12181 cgaagttaaa gacgtggctg acgtcaggaa tgtcgatacc gcgcgcggca acatcggttg
    12241 caaccagtac gtttacgcga ccttcggtca aacgcttgat cgcttcgtta cgcttgcctt
    12301 gtaccatctc accttcgaga tagcagttgt tgatgcccgc ttcgcgcagc cagtttgcca
    12361 gctcatgcac acgttcacgc ttacgcacaa acacaattga gcgggtcgct tccggctgtt
    12421 ttaacagatg caccagcaat gcggttttat gttccaggtc atcggcgcgg taataccact
    12481 gatgaatttt tttgcgctca cgggtggacg gattggcaga aacctccacc ggatcttcca
    12541 gcagacgctc ggcaaagtcc tgaatcgcat cgccttccag cgttgccgaa aagagcaggg
    12601 tctgtttacg ccagcgcgtt tcgccagcaa tatgttcgat atcctgagcg aagcccatat
    12661 ccagcatacg gtctgcttcg tcgaggatca gcgtttcaac cgcgcggcaa tcgaagttct
    12721 cttcttttat gtattgcagc agacgtccgg tcgtggcgac cacgatgtcc tgattttcgc
    12781 tgaacacttc cgcgtggttc atataggcta cgccgccggt gatggtggcg atatccagat
    12841 gcgtatgttt cgccagttcg cgggcatgat cggccacctg catcgccagc tcgcgagttg
    12901 gggtgaggat caaaatacgc ggcggaccgg atttcttacg cgggaaatcg agcaggtgct
    12961 gcaacgctgg cagcagatac gccgccgttt taccggtgcc tgtcggcgca gaaccgagta
    13021 catcacggcc atcgagcgca ggcggaatgg cggcagcctg aatggcggtc gggcgagtga
    13081 aacctttatc ctggagggct tccagcaggc tttcgtcgag ttcaagttcg gaaaaagtcg
    13141 ttacagtcat gttctacctc tgtgtggggc gctgattata gacgttacgg ctgcaatctt
    13201 catctgtttg tatggatatc gcttctcggg tattgttctc acccggtgct atcgccgttt
    13261 ttcctgcaag gttttatttt catgtcacag tctacatccg tgcttcgtcg taatggattt
    13321 acttttaaac agttttttgt tgctcacgat cgctgtgcga tgaaagtggg aacggatggc
    13381 attttgctgg gcgcatgggc accggtggct ggggtgaaac gttgccttga tatcggcgcg
    13441 ggtagcgggt tgctggcact gatgctggcg cagcgaacca gtgacagcgt gataattgat
    13501 gccgttgagc tggaaagtga agctgccgcg caggcgcagg aaaatatcaa ccagtccccg
    13561 tgggcagagc ggattaacgt ccatacggcg gatattcagc agtggatcac acagcagaca
    13621 gtacgcttcg atttaatcat cagtaaccca ccttactatc agcagggagt agagtgcgcg
    13681 acacctcaac gggaacaggc tcgctatacc actacgcttg atcacccatc gctgctgacc
    13741 tgtgcggcgg agtgcattac cgaagaggga tttttctgcg tggtgctgcc agagcagatt
    13801 ggtaatggtt ttactgaact ggcattaagc atgggctggc atttacgttt gcggacggat
    13861 gtggcggaaa acgaagcgcg actgccgcat cgggtgctgc tggcattctc cccgcaggcg
    13921 ggagaatgct ttagcgatcg cttagtcatt cgtgggccag accagaacta ttccgaagcg
    13981 tatacggcgc tgacccaggc tttttatctg tttatgtaat gattgccggg ggaaaggatc
    14041 gatggaccgg aatgcgtgag cagttccggg taatccagcg taaaatgcag cccccggctt
    14101 tctttgcgca tcatcgcgca gcgaacaatc aactcggcaa cctgtaccag attacgcagc
    14161 tccagcaaat tatttgagac gcggaaatgg gcgtaatatt cgtctatttc ttgttggagc
    14221 atggttatcc gccgcagggc gcgttccagg cgcttcgttg tgcgcacaat gccaacgtaa
    14281 tcccacataa acagccgtag ctcgtgccag ttatgctgaa ttactacccg ttcgtcaggg
    14341 ttctcaacgc ggctttcatc ccacggcggt aacgtactga cgccgtgggc atcaggcata
    14401 cgtctggtga tatcttctgc cgccgaccag ccatagacca gacactccag caatgaattc
    14461 gaggccatgc ggttagcgcc gtgtaagccg gtataactca cctcgccaat ggcatacaag
    14521 ccctcgacgt ccgtacgccc atgatcatca accattacac caccgcaggt ataatgtgca
    14581 gcaggcacaa tcggtaccgg ttcttgtgtg agatcaatcc ccagcccgag caatttttca
    14641 taaatcatcg ggaaatgctg gcgaataaaa tcggcgggct tatggctgat gtcgaggaac
    14701 atacaatctg cgccgaggcg tttcatttca tggtcaatgg cgcgggcgac aatatcgcgc
    14761 ggggccagtt cgccgcgctc atcaaaatcg ggcataaaac gcgtgccatc cgggcgcttg
    14821 agataagcgc cttcgccgcg cagtgcttcc gttaacagga aattgcgtgc ctgtgggtga
    14881 tacagtgcgg tagggtggaa ctgattaaat tcgagattgg caacccgaca gcctgcgcgc
    14941 cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta ctgataaacc
    15001 ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc caccgtttct
    15061 ttatttcggt tccataccca cgcgccaaca acccgtcgcg tgcccgggag gccaattttg
    15121 tcagaaacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt cggatggttc
    15181 tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc ggcatgaaga
    15241 atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc gccattcggt
    15301 tgaacgtggg tatcaaacaa aaccccttgg tcaattaacc attgcacaca ggatcgtgca
    15361 ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc aatcaatgtg
    15421 tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat accgccctgg
    15481 gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac ctgatgctgg
    15541 tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccgataat caacacgtca
    15601 catgaatgtt ctgggagagt attcattttc tttgtttaat ttactaaaca ccgtttggtc
    15661 agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt acagtggcta
    15721 aacacgctga cgcagggcgg cgagaaaaag agaagttact ggctggtgga ggattaggtg
    15781 gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt tctacacaga
    15841 taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt ctacagcatg
    15901 acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta accctttgct
    15961 tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt ggtttgggga
    16021 gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac gggtccagaa
    16081 gggagatcag aaagccttta acctactggt agtgcgctat cagcataaag tggcgagtct
    16141 ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag cttttattaa
    16201 agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat ggctgtatcg
    16261 gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc caccttccag
    16321 tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga aagaaatttc
    16381 gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc gaactattga
    16441 gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg gcctgagcta
    16501 tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac gtatcttccg
    16561 agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac gatagcggga
    16621 tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat ggatggcgaa
    16681 acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca gaaaacctgg
    16741 gaaagctatc acttaatccg tgactcaatg cggggtgata ctcccgaggt gctccatttc
    16801 gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca accggcgaca
    16861 ttgatcccgg aagcccagcc tgcgccgcat caatggcaga aaatgccatt ctggcagaaa
    16921 gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt atcgcttgca
    16981 gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca gcccgaaacg
    17041 ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct gggagtacct
    17101 tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg tcgtcgcatt
    17161 aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca gcttcagttt
    17221 gagcaggcgc aaacccagca agccgctgta caggtgccag gaattcaaac tttaggaacg
    17281 caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt agcctgttat
    17341 tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag atgaacttgg
    17401 ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag ggtgttgagt
    17461 ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg ttgcaaatgg
    17521 atggcccgcg ccgggaagtg gtacagcgtg gcaatgaaat cagctatttt gaaccgggac
    17581 ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg ctcatctata
    17641 ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc acgcgtattg
    17701 ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc tacagctaca
    17761 tcgtgtggat ggacatcgaa tcgaaattac cgatgagggt tgatctcctt gatcgcgatg
    17821 gtgaaacgct ggaacaattt cgcgtgattg cttttaacgt caatcaggat atcagtagca
    17881 gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct gtaggtgaaa
    17941 aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa gtttccagta
    18001 gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc tattccgacg
    18061 gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc gatcagatgt
    18121 tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa atcaccattg
    18181 tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag ttcggggcag
    18241 cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg cgctggtcag
    18301 ttgtgatgtt aaagtttcat gcagcagctg cgcttcacgc gccggttgcg gtagccgcgt
    18361 gttaaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg atgagccgtt
    18421 agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta gctccgcatt
    18481 actggtttat atgtcgccgc tggtgggatt attcctcatc gcttcgctat ttcagctact
    18541 ctttgcttct gacgtcgcag cattatgtgg tgcggtcctc ggcggtattg gcgggttcct
    18601 gattgcgcgc ggctactcgc gtaagtttgc tgcccgggca gaatggcaac cgatcatctt
    18661 aagcgtggca ttgccgcctg gccttgtgcg atttgaaaca tcttcggaag acgcgagcca
    18721 gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt ccagatacca
    18781 caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat gcggcgtttc
    18841 tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta accgggcatt
    18901 aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat agctcacatt
    18961 gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg cctgtctgac
    19021 cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg tggcataacc
    19081 atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac ctatcagctg
    19141 aactttatcg acaccccggg ccacgttgac ttctcctatg aagtttcccg ttcgctggct
    19201 gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtagaagc gcaaaccctg
    19261 gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccggtact gaacaagatt
    19321 gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat cgtcggcatc
    19381 gacgccaccg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca ggacgttctc
    19441 gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc gttgcaggca
    19501 ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat ccgtattaaa
    19561 aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca gacctataac
    19621 gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact gaaatgtggc
    19681 gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt cggcgatacc
    19741 ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa agtcaaaccg
    19801 caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt ccgtgacgcg
    19861 ctgggtaaac tcagcctgaa cgatgcttca ctgttctatg agccggaaag ctccagcgcg
    19921 ctgggctttg gtttccgctg cggcttcctc ggcctgctgc acatggagat catccaggaa
    19981 cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt agtgtatgaa
    20041 gttgaaacca cgtcaagaga agttatttac gtcgacagtc catccaagct gccagcggta
    20101 aataacatct acgaactgcg cgagccgatt gccgaatgtc acatgctgct gccgcaggca
    20161 tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac caatatggtt
    20221 taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt ggtgctcgac
    20281 ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta caacttcaaa
    20341 cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga acgtgttgat
    20401 gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga gttggtggag
    20461 aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc agcgattggt
    20521 acgcacatta ttgcgcgatc caccgtgaaa cagctacgta aaaacgtact ggctaaatgt
    20581 tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga aggtaagaaa
    20641 cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc cattctgcac
    20701 gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt tgccctgatt
    20761 ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt ctttttcgca
    20821 cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc actggataaa
    20881 gcaaccctga aaaaggttgc gccgaagcct ggctggctgg aaaccggtgc ttctgttttt
    20941 ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt ccagatcccg
    21001 tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga gaagtttgct
    21061 tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca tccgaaacgc
    21121 ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat caagcgcgcg
    21181 gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct gacgattcaa
    21241 ccgggatgca gttccggcca ggcgtgtgaa aacgcgctac cggtcaccta ctccaacgtg
    21301 gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc gaccagcgga
    21361 ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc cgagcgtaaa
    21421 gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca ggaccaggtg
    21481 gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc gccgggacaa
    21541 tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg gggttttgtg
    21601 cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga taagcaagaa
    21661 ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta atagcccatc
    21721 ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat aaatctcccg
    21781 tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt aaggttggca
    21841 cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt aaactgaaac
    21901 tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc tgaattgttg
    21961 acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt caacggaagc
    22021 tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat cgtagtgcca
    22081 gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc tacgttatcg
    22141 ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc cggatgcgcg
    22201 ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag ttaggcgagt
    22261 gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag tcaattctcg
    22321 ccgacaccgt cgaagcatta attggtggcg tattcctcga cagtgatatt caaaccgtcg
    22381 agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca ggcgataaac
    22441 aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg ccgctgccga
    22501 cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact atccactgcc
    22561 aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt aaggctgagc
    22621 aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg ataaaagtta
    22681 ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat tgttgaacaa
    22741 actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc gtcaccgcat
    22801 tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc ctggcctgca
    22861 tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca gctctattgg
    22921 cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg acgacgaaat
    22981 ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga acaaagtgga
    23041 taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa gtcagatgaa
    23101 cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca ctatcgcagc
    23161 aatcgtgcgt aagcatctcc ctgaagcgac ccatcacttc ccggaagatt acatcaccga
    23221 tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc gtttcctcgg
    23281 cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta acgaacgcgg
    23341 tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga agatggttat
    23401 tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag acatgcagga
    23461 aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg gttgggccga
    23521 cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt cactccgatg
    23581 gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac cagcctgatg
    23641 ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg cgcacgctct
    23701 aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct acgtttcggc
    23761 gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc gctgccatta
    23821 agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg cgtactggaa
    23881 tacgagacgc gcttctctga actctttttc gattacttgc actgcattca gtctcttgca
    23941 ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact gctcgggcat
    24001 ctgggctatg gcgtcaattt tacccattgt gcgggtagcg gcgagccggt agatgacacc
    24061 atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat cgacaataaa
    24121 acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga cgcagacaca
    24181 ctacgcgccg cgaaacgctt tacctgcatg gcgcttaagc cgtatcttgg cggtaaacca
    24241 ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa aacacattat
    24301 gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac catattgcaa
    24361 cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg tttattgctg
    24421 agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc cacattactg
    24481 accgtgatgt gcgcatcctg cgtcagacgc tggatacccg catgaatctg gagatggcgg
    24541 tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc tgtctggtac
    24601 cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgtcgcaggg cagcgtgaca
    24661 aaatgcgcga tgcctgcaaa cgtctggcag atgccgggat ccaggtttct ctctttatcg
    24721 acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt atcgagatcc
    24781 acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag ctggcgcgta
    24841 tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgct ggtcacggtc
    24901 tgacctatca caacgtgaaa gctattgccg ccatccctga gatgcatgaa ctgaatatcg
    24961 gtcatgccat tattggtcgt gcagtgatga ccggactgaa agatgcggtg gcagaaatga
    25021 agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca cggatatcgt
    25081 agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg cacgccgcgt
    25141 attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc gttttctggc
    25201 gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga tccgcaatgg
    25261 tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac ggctacggct
    25321 atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc atgtaacgct
    25381 ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa ataataccgt
    25441 tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact ttttaacttt
    25501 aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac ttatctttta
    25561 caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag caatattgat
    25621 ggaaagatta acgtgaccgc caattcgtaa gtacattaaa attggcttcg ttattgaaga
    25681 ttttgctgtg ctttacacca tgccacagaa ttcccccatt gaaacgagtg gtgtcgtcaa
    25741 agctctggtg tggagtgcag catgcaccct caataactcg cacgttcagt tttggggaga
    25801 tgtaagggct aatctgaacg gctgcattcc ttgtttaagg aaaaacgaat gactgattgc
    25861 cgatacctga ttaaacgggt catcaaaatc atcattgctg ttttacagct gatccttctg
    25921 ttcttataac acaaggaaac gtacttaagg tgcgtccggt gaaccaatcg gacgcgcctt
    25981 taataactat aaataagtgt cttggcagat actatataaa ttaacttagt gaatgattat
    26041 gctaatgtca tcaattaaat aaatataatg gcgttaaggc ttcccagtaa tataattaat
    26101 actctacttc cagagtagaa tattaaattt tatccgcgtg gtgcatcagc acaaatttat
    26161 cccacaactg ttcttctgtc tcgacatgcg ccggatcttt cacaatagta ttggggatcg
    26221 ggcacacctt ctggcaggtt ggtgtctcgt agtgccctac gcattcggta cacttatcgc
    26281 tgttaatctc gtagatatga tctcccattg aaatcgcctc attcgggcat tcgggttcac
    26341 acatatcaca attgatgcat tttttagtga ttaacaacgc cataaggaaa ctctcaaaac
    26401 aacacaaacc gggcgggcat tatacgcgca ttctttactc agaccagttt tttcaccagc
    26461 gcttcactat gacgaatacg ttctggtgcc agttccagat cctgctgaat cagcgcaatg
    26521 aacagcaaat ccgttaacat tccctgagcg tgacaagcag agattgaagc gctgtttgtc
    26581 gcttgttctt cggcaatggt atacaggcaa tgagaagcac gctgttgcag ggcattcgga
    26641 gtaaagccgg taatcgccag cacttttccg cccactcgca gcatctcatc tgccgccagg
    26701 tttaactcgc gtcgtacacc ggtgtaggaa atggctaata acagatcgtc aggggacgac
    26761 gcctgtactg ttgcgagtag cgcatgcata tcgcgcactg cggcagcatt gaagccaatc
    26821 ttcatcagct tccaggcaaa gttttgcgcc accagacccg aagcgccaat accggtcaga
    26881 attatccgcc gcgcagagcg caacattgtt acgcattcat gcagtttctc ttcactatta
    26941 acgtttagcg ttgcgtacat agcggcagta ttttctttaa tcagtttttc gccgaccagc
    27001 cgtaacggat catcaccgcg gatttggtta tgaatgggca cggagggcga ttccggctgg
    27061 cttgccagcg cttcactcaa cgccaactta agcgccggaa aacctttata gccgagtttt
    27121 tgcgcgaact tcacgacgct ggactgactg actccggctt cgttggccag ttgctgagag
    27181 cttaaatggc gcgccgtatc aggttgtagc agcagataat ccgccagttt tttatcactc
    27241 tgggcaagcc cctggtaacg ctgacggata cgaagtaagc cgttcatatc tttcctcgcg
    27301 atgaaatagt ggcgacaaca ggcaatatga gagacacgcg agtgaatttt atattccatt
    27361 gccgctaatt gccatgaatt aaaaattcca gttagccatg atgcggtcga ttgttgccgg
    27421 aaaagtgatg atctatctgt taggctatgt agtagcgaaa attaatcagg atgtttcagt
    27481 ccagaggagt atggtttggc aactcacgag cgtcgtgtgg tgttttttga cttagatgga
    27541 acattgcatc agcaggatat gttcggcagt tttctgcgct atttactgcg tcgccaaccg
    27601 ctgaatgcgt tacttgtcct gccgttgtta ccgattatag ccattgcgtt attgataaaa
    27661 ggtcgtgcgg cacgctggcc gatgagtctg cttctgtggg ggtgcacttt tggtcacagc
    27721 gaagcacgtt tacagacgtt gcaggccgat ttcgtgcgct ggtttcgcga caatgttacc
    27781 gcctttccgc tggttcagga gcgattaacc acctacctgt taagttccga tgctgatatc
    27841 tggttgatta ccggctctcc gcagccgctg gttgaagcgg tttatttcga tacgccctgg
    27901 ctgccgcggg ttaatcttat cgccagccaa attcagcgtg gctatggtgg ttgggtattg
    27961 acgatgcgtt gtctgggaca tgaaaaggtc gcacaactgg agcgcaaaat cggcactccg
    28021 ctgcggctgt acagtggcta tagcgacagt aatcaggaca atccgctgct ttatttctgt
    28081 cagcatcgtt ggcgagtaac cccgcgcggt gaactccagc aactggaata gagtaaagca
    28141 tagcgtccgt gtataatgcg ccgcgctttt ataaccggag ttttcttttt gtctgaagtc
    28201 gaatttagcc acgaatactg gatgcgtcac gcgctgacgc tggcgaaacg tgcctgggat
    28261 gagcgggaag tgccggtcgg cgcggtatta gtgcataaca atcgggtaat cggcgaaggc
    28321 tggaaccgcc cgattggtcg ccatgatccc accgcacatg cagaaatcat ggccctgcgg
    28381 cagggtggtc tggtgatgca aaattatcgt ctgatcgacg ccacgttgta tgtcacgctt
    28441 gaaccatgtg taatgtgtgc cggagcgatg atccacagtc gcattggtcg cgtggtcttt
    28501 ggtgcgcgtg acgcgaaaac tggcgctgcg ggatctttaa tggatgtgct gcatcatccg
    28561 ggtatgaatc accgagtgga aattacggaa ggaatactgg cggatgagtg cgcggcgttg
    28621 ctcagtgact tctttcgcat gcgccgccag gaaattaaag cgcagaaaaa agcgcaatcc
    28681 tcgacggatt aattttgttt ctcttcactc cctttcctgg aaaacagcag agagggagaa
    28741 tgggtcaaat agtttgatga cgactgggaa agaaacgaga gaaaaggaaa tttctctttg
    28801 cccaactccg taggcgatac cgccggataa tcctgcgcca gttgcatcgc cgcttctgta
    28861 gcctgcttct ctttctcttg cagataaccc accaggctaa tctgatactt acgaatattt
    28921 tcgacataag cgtaggcttc atgtccacga gcgtagccgt aagtcagctt gctgtaatag
    28981 ggtttctggc taagtaaagg cagacgctgt tttacgtcag cccaactgtc aggattccct
    29041 ttggtttttg ccgtcagggc gcgggcatcc agcatatgcg catagcccat attgtacgca
    29101 gcgagggcaa accagatccg ctcgttctcc ggcacacttt ccggcacttt actcatcata
    29161 tcctgcaaat aacgcacgcc accgctgatg ctctgttcgg catcggtacg atccgtaatg
    29221 ccgaggcttt gcgcggtatt tttggttaac atcatcatgc cgcgcacacc cgtcggtgaa
    29281 gtggcctgtg catcccagtg cgattcctga taagcaatag cggccagcaa acgccagtca
    29341 atttcttcgg cgtatttctc aaacaggggc tttaactgcg gcagtaccgc atcgacggcg
    29401 cgtaaaaatg tgcgcgtatc gacgtaatca aaatcatcgc catgccccag gtatttctct
    29461 tcaatgcgtg ccagcgtacc gtcttcattc atttcgttga agaagtcgag cagggcggcg
    29521 gaaagggtat tatcgccatc taacgggcta aaccaagtca ccggttgttc atcggtgata
    29581 tcgagcgcta cggcgagctc cgggtgaacg cgctgaaaca ggctgatggc gacagaatca
    29641 gcaatggtgt aatcgagttt tccttcgatg acatcttcca ttaattccgc agagcctttt
    29701 ttgtcgtcta ccttccagct taattccggg aattttgttt ctttcagggt ctggagatcg
    29761 ttaaccacca catgacccgg tgcaacggtg agttgctccg ccgtcaggtt gcccagcgta
    29821 cgtgggcgat actgacccac tttataaacc agttgttgtg acacggaata ataggtaggg
    29881 ccaggctgat aattttttac ccgctcactg ttatagacca gtcctgccgc cagcaggtcg
    29941 gcgttaccat tatcaaggtc gtcaaacagc tggctgatat tctgccgcac ggtcactttc
    30001 agttttacgc cgaggtaatc ggcaaactgt ttcgccagtt cgtaatccag gccaaaaggt
    30061 ttcccgttga tttcgttata agtcagggga gtatgaatgg tgctcacacg caactctccc
    30121 cgcgcttgaa tggcggcgat acggttgtcg gctttaccaa accagggaat ggatggccag
    30181 agagcgaccg cgagcagcag tgccagaatg ccgatgaaca gataattaat ctttaatttt
    30241 ttcaattagt taattctctg tgtcgtgcgc gtcccagctt gaaaaaacgt aataatagtg
    30301 aaaggtttac tcttaaatga gcggcatttt gcgtaaacct gcgccagatg gcaacttatt
    30361 acagccattg gcggcacgcg ttgctaattc acgatggtga ttttatttcc acgcaaacgg
    30421 tttcgtcagc gcatcagatt ctttataatg acgcccgttt cccccccttg ggtacaccga
    30481 aagcttagaa gacgagagac ttatgatgga aattctgcgt ggttcgcctg cactgtcggc
    30541 attccgaatc aacaaactgc tggcacgttt tcaggctgcc aggctcccgg ttcacaatat
    30601 ttacgccgag tatgtccatt ttgctgacct caatgcgccg ttaaacgatg atgagcacgc
    30661 acaacttgaa cgcctgctga aatatggccc ggcactcgcc agccacgccc cgcaaggcaa
    30721 actcctgctg gtgaccccgc gtcctggcac catctctccc tggtcttcga aagcgaccga
    30781 tattgcccat aactgcgggc tacaacaggt aaaccgcctt gagcgcggcg ttgcttacta
    30841 tatagaagcc ggtacgctga ccaatgaaca atggcagcag gttaccgctg aactgcacga
    30901 ccgcatgatg gaaacggtct tttttgcttt agatgatgca gagcagctgt ttgcacacca
    30961 tcaaccgact ccggtcacca gcgttgattt gctggggcag ggccgtcagg cgctaatcga
    31021 cgctaacctg cgtcttggcc tggcactggc ggaagatgaa attgactatc tgcaggatgc
    31081 tttcacaaag cttggtcgta acccgaacga catcgagctg tatatgttcg cccaggcgaa
    31141 ctccgagcac tgccgccaca agatttttaa cgccgattgg gttatcgacg gtgagcaaca
    31201 gccgaaatcg ctgtttaaga tgatcaaaaa caccttcgaa accacgccag attacgttct
    31261 ctctgcttat aaagataacg ccgccgtaat ggaaggttct gaagtgggcc gctactttgc
    31321 tgaccacgaa acgggccgct acgatttcca tcaggaaccg gcgcatattc tgatgaaagt
    31381 cgaaactcac aaccacccga cggcgatttc tccgtggccg ggcgcggcga ccggttccgg
    31441 cggtgaaatc cgcgatgaag gtgccaccgg gcgcggcgcg aagccgaaag cgggtttggt
    31501 tggtttctcc gtttccaacc tgcgaattcc tggcttcgaa cagccgtggg aagaagattt
    31561 cggtaagcct gagcgcattg tcaccgcgct ggacatcatg accgaaggcc cgctgggcgg
    31621 cgcggcgttt aacaacgaat ttggtcgtcc ggcactgaat ggctacttcc gtacttatga
    31681 agaaaaagtg aacagccaca acggcgaaga gctgcgcggt tatcacaaac cgatcatgct
    31741 ggcgggcggg atcggcaaca ttcgcgccga tcacgtacaa aaaggcgaga tcaacgtcgg
    31801 tgcgaagctg gtcgttctcg gcggcccggc aatgaacatc ggtcttggcg gcggagcggc
    31861 gtcttccatg gcgtcaggtc agtctgatgc cgacctcgac tttgcttccg tacagcgcga
    31921 caacccggag atggagcgtc gttgccagga agtgatcgac cgttgctggc agcttggtga
    31981 tgctaaccca attctgttta tccacgacgt cggtgcgggc ggtctttcta acgcgatgcc
    32041 ggagctggtg agcgacggcg gacgcggcgg taaatttgaa ctgcgcgata ttctaagcga
    32101 cgaaccgggc atgagcccgc tggaaatctg gtgtaacgaa tcccaggaac gctacgtgct
    32161 ggcggttgct gccgatcaat taccgctgtt tgacgaactg tgtaagcgtg agcgcgcacc
    32221 ctacgcggtg attggtgaag cgaccgaaga actgtatctt tctctgcacg atcgtcattt
    32281 tgataatcag ccgatcgatc tgccgctgga cgtcctgctt ggtaaaacgc cgaagatgac
    32341 ccgcgatgta caaacgctga aagcgaaggg cgatgcgctg gtccgtgaag ggatcaccat
    32401 tgcagacgcg gtgaaacgcg tactgcatct gccgaccgtg gcggagaaaa ctttcctggt
    32461 gaccatcggt gaccgcagcg taactggcat ggtagcgcgc gatcagatgg tgggtccgtg
    32521 gcaggtgccg gtcgctaact gcgcggtcac taccgccagc ctcgacagct actacggtga
    32581 agcgatggcg attggcgagc gtgcgccggt tgcgctgctg gatttcgccg cctctgcccg
    32641 tctggcggtc ggtgaagcgt taaccaatat tgccgcaaca cagattggcg atatcaaacg
    32701 cattaaactt tccgccaact ggatggcggc ggcaggccac cctggtgaag atgcgggcct
    32761 gtatgaagcc gttaaagccg tgggcgaaga gctttgtccg gcgctgggcc tgacgatccc
    32821 ggtgggtaaa gactctatgt cgatgaaaac ccgctggcag gaaggtaacg aagagcgcga
    32881 aatgacgtcg ccgctgtcgc tggtgatttc tgcatttgcc cgcgtggaag atgtacgtca
    32941 caccatcacg ccgcagcttt ctaccgaaga taacgcactg ctgctgattg atttgggcaa
    33001 aggcaataac gcgctgggcg caacggcgct ggcgcaggtt tatcgtcagc ttggcgacaa
    33061 accggcagat gtacgcgatg tcgcgcaact gaaaggcttc tatgacgcga ttcaggcgct
    33121 ggttgcacag cgtaagctgc tggcgtatca tgaccgctct gatggcggtc tgttggtcac
    33181 actggcggaa atggcctttg ctggtcattg tggcattgat gcggatatcg gctctctggg
    33241 tgacgatcgc ctggcggcgt tgtttaacga agaactgggt gcggtgattc aggttcgtgc
    33301 cgctgaccgt gaagcggtcg agtccgtact ggcacagcat gggcttgctg attgcgtcca
    33361 ttatgttggg caggcggttt ccggtgaccg ttttgtgatt accgccaacg ggcagactgt
    33421 attcagcgaa agccgcacta cgttgcgtgt ctggtgggca gaaactacct ggcagatgca
    33481 gcgcctgcgt gacaacccgg agtgtgctga ccaggagcac caggcgaaat caaacgacgc
    33541 cgatccgggc ctgaatgtaa aactgtcgtt cgatatcaac gaagatgtgg cagcaccgtt
    33601 tattgccact ggcgcgcgcc cgaaagttgc cgtactgcgt gagcagggcg tgaactcgca
    33661 tgttgaaatg gcggcagctt tccaccgtgc aggctttgat gctatcgacg tgcatatgag
    33721 tgacctgctg gctggacgca cgggcctgga agatttccac gccctggtcg cgtgcggtgg
    33781 tttctcctac ggtgatgtgc tgggtgccgg tgaaggttgg gcgaagtcaa tcctgttcaa
    33841 tgaccgtgta cgcgatgagt ttgcaacctt cttccaccgt ccgcaaacgc tggcgctggg
    33901 ggtatgtaac ggttgccaga tgatgtctaa tctgcgtgaa ctgatcccag gtagtgagtt
    33961 gtggccacgt tttgtgcgca atacctccga tcgctttgaa gcgcgtttca gcctggttga
    34021 agtgacccaa agcccgtcgc tgctgttgca ggggatggtg ggctcgcaaa tgccgattgc
    34081 tgtctctcac ggtgaagggc gcgtggaagt gcgtgatgcg gcgcatctgg cggcactgga
    34141 aagcaaaggg ctggtggcac tgcgctatgt cgataacttc ggcaaagtca ctgaaaccta
    34201 cccggctaac ccgaacggtt ctccgaacgg tattacgtcg gttacgactg aaagtggtcg
    34261 agtcaccatt atgatgccgc acccggaacg tgttttccgt actgtcagca actcctggca
    34321 tccggaaaac tggggcgagg atggcccatg gatgcgcatt ttccgcaatg cgcgtaagca
    34381 gttggggtaa gtcatcagtt cattggtttt gcagcccgga tgcggcattt acgccgcatc
    34441 cggcattaag caccaaccac tatcataagc tcccaactcc gggagctttt ttgtgtctgt
    34501 aaatcacgac aatgggtggt ttaccgtgtc gctttctggc gacacttaaa tgattgattt
    34561 tattgtaatc taataagttt atcttaagtt gtctcttttt agcgacacag tggctgattt
    34621 acattcaaat agcgaagaag ccataaatcc ctcaaagcaa cgtataatca gtacgttacc
    34681 aaactatttt ctttattggc acagttactg cataatagta agcagtggct cattcaccga
    34741 cttatgtcag ccccttcggg acgtgctaca taaaattcga atgacgcaca acaaggtgcc
    34801 tgccgtccaa cttctgatat cagcgtagct atatcaacca tcgggcgaaa cgtcgagtta
    34861 ggcaccgcct tattccataa caacgccggg taattcccgg ctttgttgta tctgaacttc
    34921 ccctcggtta gcatcaggct attcgcgtct gacgagagta acaccttgaa acgctggccc
    34981 gtttttcccc gctcattacg acaactggta atgctggcat ttttgctaat tctactaccc
    35041 ctgttggtgc tggcatggca agcctggcaa agcctgaatg cgcttagcga tcaggcggcg
    35101 ctggttaacc gcactacgct tattgatgcc cggcgtagtg aagcgatgac caacgcggcg
    35161 ctggagatgg agcgtagcta ccgtcagtat tgcgtgctgg acgaccctac gcttgcgaag
    35221 gtttatcaaa gccagcgcaa gcgttacagc gaaatgctcg atgcccacgc aggcgtactg
    35281 ccagacgata aactttacca ggcattgcgt caggatttga acaatctggc tcaacttcag
    35341 tgtaacaaca gtggtcccga tgctgccgcc gccgcgcgtc ttgaagcctt tgccagtgcc
    35401 aataccgaaa tggtgcaggc cacgcgcaca gtggtgttct ctcgtgggca gcaacttcag
    35461 cgtgaaatcg ccgaacgtgg gcaatatttt ggttggcaat cgctggtgct atttctggtg
    35521 agtctggtga tggtgctact tttcacgcgg atgattatcg ggccggtgaa aaatatcgag
    35581 cgcatgatca accgtctggg ggaagggcgt tctctgggca atagcgtttc gttcagtgga
    35641 ccgagcgagt tacgctcggt tgggcaacgt attctttggt taagtgagcg cctgtcatgg
    35701 ctggaatccc aacgccatca atttttaaga catttatctc atgaattaaa aacgccactg
    35761 gcgagtatgc gcgagggcac tgaattactg gctgaccagg ttgtcgggcc gcttacgcca
    35821 gagcaaaaag aggtggtgag cattcttgat agcagcagcc gcaatttgca aaaactgatc
    35881 gaacaactgc ttgattacaa ccgtaaacag gcggacagtg cggtggaact ggagaatgtt
    35941 gaattagcac cgctggtgga gacagtggtt tctgctcata gcctgcccgc acgggctaaa
    36001 atgatgcata ccgacgtcga tctcaaagca acagcttgcc tggcggagcc aatgctgctg
    36061 atgagcgtac tggataatct ttactccaat gcggtgcact acggggctga atccggtaac
    36121 atttgccttc gcagcagctt acatggtgca cgggtttata ttgatgtcat caatacaggc
    36181 acacccattc cgcaagagga acgcgccatg atcttcgaac ccttttttca aggaagccac
    36241 cagcgaaaag gggcggtgaa gggcagcggt ctgggattaa gcattgccag ggattgtatt
    36301 cgccgtatgc aaggggaact gtatctggtc gacgagagcg ggcaagacgt ttgtttccgc
    36361 attgaattgc cgtcgtcgaa aaacacgaaa taatgctaac agcctatccc atcaggctat
    36421 tttatttgcc agttcggccc cgggcagtgc tcgaaatcct cacgtacgac gtgtacgctc
    36481 cggtttctcc gcgctgtcca tgtccaaact gcctgcaata attacgccca ctgggatagg
    36541 cattaagtct ggtgaatatg cgacacattt ttcaacgatt attgccccga cggctgtggc
    36601 tggcagggct gccatgtctg gcattgctgg gttgcgtgca gagtcacaat aagccagcca
    36661 ttgatacgcc agcagaagaa aaaattccgg tttatcaact ggctgactat ctttctactg
    36721 aatgtagcga catctgggcg ctgcaaggta aatcaaccga aaccaatccg ctttactggc
    36781 tgcgggcgat ggattgtgct gatcgtttaa tgcctgcgca gtcacgtcag caagctcgcc
    36841 aatatgacga cggcagctgg caaaatacct ttaaacaagg gattttgctc gccgacgcca
    36901 aaattacgcc gtatgagcgc cgccagctgg tcgcgcgcat tgatgcgcta agtaccgaga
    36961 tcccggcaca ggttcgccct ctgtatcaac tctggcgcga cggtcaggcg ctgcaactgc
    37021 aactggcgga agagcgccaa cgctacggca agctccagca ggccagcgac agcgagctgg
    37081 atacattgcg ccagcaacat cacgttctac agcagcaact ggaactcacc acccgcaagc
    37141 tggaaaacct gactgatatt gaacgccagc tctcgacccg caaaccggct ggaaatttct
    37201 cgccggatac gccgcacgag ggtgaaaagc ccgcgccatc cacccatgag gtcactcctg
    37261 atgagccata aacctgcgca tttattattg gtcgatgacg atccgggatt gctgaaactg
    37321 cttggcctgc gcctgaccag cgaaggctac agcgtggtca cggcggaaag tggcgctgaa
    37381 ggattacggg tactgaatcg cgaaaaagta gatttagtca tcagcgacct gcggatggat
    37441 gaaatggacg gtatgcagct gtttgctgaa attcagaaag tgcagccggg aatgccagta
    37501 attattctta ccgcgcatgg ttctattccc gatgccgttg ctgcaacaca gcagggcgtg
    37561 ttcagtttcc tcaccaagcc tgtcgataaa gatgcgctat atcaggcaat tgacgatgcg
    37621 ctggaacaat ccgcgccagc caccgatgaa cgctggcgcg aggcgattgt cacccgcagc
    37681 ccgctgatgc tgcgtttgct ggaacaggcg cggctggtgg cgcaatcaga cgttagcgtt
    37741 ttgattaacg gccagagcgg caccgggaaa gagattttcg cccaggctat ccacaacgcc
    37801 agcccgcgca atagcaaacc atttattgct attaactgtg gcgcattgcc cgagcaattg
    37861 ctggagtcgg agctgtttgg tcatgcgcgt ggcgcgttta ctggcgctgt cagcaatcgc
    37921 gaaggtttat tccaggcggc ggaaggcggt acgctatttc tcgatgagat tggcgatatg
    37981 cccgcaccgt tgcaggtcaa actgctgcgc gtgttgcagg agcgtaaagt gcgcccgctg
    38041 ggcagtaacc gcgatattga tatcgacgtg cggattattt ccgccactca ccgcgacctg
    38101 ccaaaagcga tggcgcgcgg ggaattccgt gaagacctct attaccgcct caacgttgtc
    38161 agcctgaaaa ttccggctct ggcagagcgc acagaagaca ttccgcttct ggcaaatcac
    38221 ctgttgcgcc aggcggcaga gcgacataaa ccgtttgtcc gcgcgttctc cactgatgcg
    38281 atgaaacgcc tgatgaccgc tagctggccg ggtaatgtgc gccagttggt caacgtgatt
    38341 gaacagtgcg tggcgctgac ctcatctccg gttattagcg atgcattagt agagcaggcg
    38401 ctggagggtg aaaatacggc gctgccaacc tttgttgagg cacgtaatca gtttgaactc
    38461 aactatttgc gtaagctgct gcaaatcacc aaaggcaacg tcacacacgc ggcgagaatg
    38521 gcggggcgca accggacaga attttataaa ctgctttccc gacacgagct ggatgcaaac
    38581 gatttcaagg aatgaattgg cgttatgtgt tacgtttagc agatcaaaag acaggcgacc
    38641 ttttcaagga atagcatgaa aaagattgat gcgattatta aacccttcaa gctggacgat
    38701 gttcgcgaag cactggctga agtcggcatt accggcatga cggtcactga ggtgaaaggt
    38761 tttggccgcc agaaaggcca taccgagctg taccgcggcg cggagtatat ggtggatttt
    38821 ctgccgaaag tgaaaattga gattgtcgta ccggacgaca ttgtcgatac ctgtgttgat
    38881 accattattc gcacggcgca aaccggcaaa atcggtgacg gtaaaatctt cgtcttcgac
    38941 gtggcacggg tcattcgcat ccgtaccggt gaagaggacg acgcggcaat ttaaaccgcg
    39001 tcaagggttg ccggatgttt ccatccggca acatcaaatt acagcacctt atgcgggcca
    39061 aagcattcgt aatgaatgtt ttcctgcttc acgcccagat ccactaactg tttcgcggta
    39121 aactgcatga agccaaccgg gccgcagaga tagaactgca ttgtcggatc gctgaacgca
    39181 ccttccagtt tgctcaaatc catcagacct tcgctatcaa actgaccttt agcgcgatcg
    39241 gcttcgctcg gctgacgata ccaggtgtgc gcggtaaagc gcggcagtga ctgccccagt
    39301 tccttaactt catcagcaaa ggcgtgaaca tcgccatttt ctgccgcatg gaaccagttc
    39361 acttgtgctg tgtggcctgc ttttgccagc gtgtcgagca ttgccagcat tggcgtttga
    39421 ccaacaccgg cagagattaa cgtcactggt gtgtcatctg cgacagccat aaagaaatca
    39481 cctgccggag cgaccagttt cacgacatcg ccaacattgg cgtgattgtg caaccagttg
    39541 gatacctgcc caccctcttc gcgtttcacc gcaatacgat agcctttgcc atccggtttg
    39601 cgagtcaaag agtactgacg aatttcctga tgcgggaaac cttccggctt cagccagacg
    39661 ccgagatatt gccccggacg gtattctgcc actgcgccac cgtcgaccgg ctccagttcg
    39721 aagctggtga taagcgcgct gcgcggtgtt ttagccacaa tgcggaaatc gcgagtacct
    39781 tcccaaccac cggctttgct ggcgttttcg ttatagattt ccgcctcgcg attgataaat
    39841 acattagcca gtacaccata ggctttaccc cacgcgtcca gcacttcctg ccccgggctg
    39901 aacatttcgt ccagcgttgc caacaggtgt tcaccgacga tgttgtactg ttccggtttg
    39961 atctggaagc tggtgtgctt ctgcgcgatt ttttctaccg ctggcagcag cgcaggcagg
    40021 ttttcaatat tactggcgta ggcggcaata gcgttaaaca gggcttcacg ttgatcgcca
    40081 ttacgctggt tactcatgtt aaaaatttct ttgagttctg ggttatgagt aaacatacgg
    40141 tcgtagaaat gggcggttaa ctttggcccc gtttccacca gtaaagggat ggtggctttt
    40201 actgtagcga tggtttgagc gtcaagcata tggtcttcct ttttttgcat cttaattgat
    40261 gtatctcaaa tgcatcttat aaaaaatagc cctgcaatgt aaatggttct ttggtgtttt
    40321 tcagaaagaa tgtgatgaag tgaaaaattt gcatcacaaa cctgaaaaga aatccgtttc
    40381 cggttgcaag ctctttattc tccaaagcct tgcgtagcct gaaggtaatc gtttgcgtaa
    40441 attcctttgt caagacctgt tatcgcacaa tgattcggtt atactgttcg ccgttgtcca
    40501 acaggaccgc ctataaaggc caaaaatttt attgttagct gagtcaggag atgcggatgt
    40561 taaagcgtga aatgaacatt gccgattatg atgccgaact gtggcaggct atggagcagg
    40621 aaaaagtacg tcaggaagag cacatcgaac tgatcgcctc cgaaaactac accagcccgc
    40681 gcgtaatgca ggcgcagggt tctcagctga ccaacaaata tgctgaaggt tatccgggca
    40741 aacgctacta cggcggttgc gagtatgttg atatcgttga acaactggcg atcgatcgtg
    40801 cgaaagaact gttcggcgct gactacgcta acgtccagcc gcactccggc tcccaggcta
    40861 actttgcggt ctacaccgcg ctgctggaac caggtgatac cgttctgggt atgaacctgg
    40921 cgcatggcgg tcacctgact cacggttctc cggttaactt ctccggtaaa ctgtacaaca
    40981 tcgttcctta cggtatcgat gctaccggtc atatcgacta cgccgatctg gaaaaacaag
    41041 ccaaagaaca caagccgaaa atgattatcg gtggtttctc tgcatattcc ggcgtggtgg
    41101 actgggcgaa aatgcgtgaa atcgctgaca gcatcggtgc ttacctgttc gttgatatgg
    41161 cgcacgttgc gggcctggtt gctgctggcg tctacccgaa cccggttcct catgctcacg
    41221 ttgttactac caccactcac aaaaccctgg cgggtccgcg cggcggcctg atcttggcga
    41281 aaggtggtag cgaagagctg tacaaaaaac tgaactctgc cgttttccct ggtggtcagg
    41341 gcggtccgtt gatgcacgta atcgccggta aagcggttgc tctgaaagaa gcgatggagc
    41401 ctgagttcaa aacttaccag cagcaggtcg cgaaaaacgc taaagcgatg gtagaagtgt
    41461 tcctcgagcg cggctacaaa gtggtttccg gcggcactga taaccacctg ttcctggttg
    41521 atctggttga taaaaacctg accggtaaag aagcagacgc cgctctgggc cgtgctaaca
    41581 tcaccgtcaa caaaaacagc gtaccgaacg atccgaagag cccgtttgtg acctccggta
    41641 ttcgcgtggg tactccggca attacgcgtc gcggcttcaa agaagcagaa gcgaaagaac
    41701 tggctggctg gatgtgtgac gtgctggaca gcatcaatga tgaagctgtt atcgagcgca
    41761 tcaaaggtaa agttctcgac atctgcgcac gttatccggt ttacgcataa gcgaaacggt
    41821 taatctgttg aaaaaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc
    41881 tgccgtaact gcgcctcaac atacaaatag ccaattccca gcacctgttg tgcgcggctt
    41941 aattgcccga agccaatttg cgtcgctttc acgctcggtc cttcgtcgcg gtcgaacggg
    42001 ttaaatcctg tctggcgaat aatagtatta agccagttct caccaaaggc acaactgcga
    42061 ccgtacaacc aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca
    42121 atggcattgg cactgcggtc aacccagctg gtgatccacg gttctccact ttgccaggca
    42181 gcgattaact gcgctgtagt cagtttttca ggatcaagtt gagtactaac cggttgtgat
    42241 tttagccata cccgcgcctg tttttttaat gcgcttaacg aggcgacggt ttccaggcag
    42301 ccataacgtc cacagtcgca gacgacgcca tccggattaa cgatggtgtg accaatctgt
    42361 ccgctaccat acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg
    42421 ccgtaatcaa cgttgatcac gcagaaatcc cgttcctgcg aattattttg ccatttctcc
    42481 gccagcgcca gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc
    42541 agcagatatt tcacctccac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg
    42601 ccggtcacag gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc
    42661 cacaatttac ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc
    42721 ggtgcatcaa tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc
    42781 tgacactcaa tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca
    42841 atcagccatg taccgctact atgcccgcgc gtctggcttt catcgtcaat attcaccacc
    42901 cgtttttcgc tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc
    42961 agccgggcca gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg
    43021 catttgttat ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct
    43081 tgtttagtta attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc
    43141 acaaatcttt taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag
    43201 tgtggcaaga gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca
    43261 tgttcctcga aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga
    43321 ccgatacgct gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg
    43381 actacatcaa agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata
    43441 aagtgaaaca gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc
    43501 tgctggggcc gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga
    43561 ccacctttat gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt
    43621 gggtaggcga aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc
    43681 ctgaccgggc ggcgctggaa atcgccagcc gcgtctataa cggtaacgcc acgccgcgtc
    43741 atttcttgtg gtgggccaac ccggcagtga aaggggggga agggcatcag agcgttttcc
    43801 caccggatgt gactgcggtg tttgatcacg gcaaacgggc cgtctccgct ttccccatcg
    43861 ccaccggcac ttactacaaa gtggactact ccgccggagt ggacatttct cgctataaaa
    43921 atgtgcctgt tccaacctca tatatggccg aaaaatcgca gtacgatttt gtcggcgcgt
    43981 ggtgtcacga tgaagatggc ggtttgctgc acgttgccaa ccaccatatt gcgccaggta
    44041 aaaaacagtg gagttgggga cacagtgaat ttggccaggc gtgggataag agtctgaccg
    44101 acaataacgg tccgtatatc gaactgatga ccggtatttt tgccgataac cagcccgatt
    44161 ttacctagct tgatgcttac gaagagaagc gttttgagca gtatttcctg ccttatcatt
    44221 ctttgggcat ggtgcaaaat gcctcccgcg atgcggtgat aaaactccag cgtagtaagc
    44281 gggggattga gtgggggctg tatgccatct ctccgttgaa cggataccgc ctggcgatcc
    44341 gcgaaatcgg caaatgcaac gcgttacttg atgatgccgt ggcactgatg cctgcgaccg
    44401 ccatccaggg cgtgttgcac ggtatcaatc ctgaaaggct gaccattgag ctctccgatg
    44461 ccgacggcaa tattgtactg agttatcagg aacatcagcc gcaagagttg ccgttgccgg
    44521 acgtcgccaa agcgccactg gcagcacaag acattaccag tacagatgaa gcctggttta
    44581 tcggtcagca tctggagcaa tatcatcacg ccagccgttc accgttcgat tactacctgc
    44641 gcggcgtggc gctggatccg ctggattacc gctgtaacct ggcgctggcg atgctggaat
    44701 ataaccgcgc cgatttcccg caagcggtgg cgtatgccac tcaggctctg aaacgcgcac
    44761 atgcgctgaa caaaaatccg cagtgcggac aggcgagttt gattcgcgcc agtgcttacg
    44821 aacgtcaggg acaatatcaa caagccgaag aggatttctg gcgtgcggtc tggagcggca
    44881 acagcaaagc cggtggctat tatggcctgg cacgactggc ggcgcgtaat ggtaacttcg
    44941 acgctggtct ggatttttgc caacaaagtc ttcgcgcctg cccaaccaat caggaagtgc
    45001 tttgcctgca taatctgctg ctggtgttaa gtggtcgtca ggacaacgcg cgtgtgcagc
    45061 gcgagaaact gctgcgcgat tatccgctga acgccactct gtggtggctg aactggttcg
    45121 atggtcgtag cgaatcagct ctcgcgcagt ggcgcggtct gtgtcaggga cgcgacgtta
    45181 acgccctgat gaccgccggg caactgatta actggggaat gcccaccctg gcggcagaga
    45241 tgctgaacgc actggactgc cagcgcacgc tgccgcttta cctgcaagcc agcttgctgc
    45301 cgaaagccga acgtggcgaa ctggtcgcaa aagccattga tgtcttcccg cagtttgtcc
    45361 gtttcccgaa tacgctggaa gaagtggcgg cgctggagag tattgaagag tgctggtttg
    45421 ctcgccattt actggcctgc ttctactaca acaaacgtag ctacaacaaa gccattgcct
    45481 tatggcaacg ttgcgtagag atgtcgccgg agtttgccga cggctggcgc gggttagcga
    45541 tccatgcgtg gaataagcaa cacgattatg agctggccgc gcgttatctt gataatgctt
    45601 atcagcttgc gccgcaggat gcacgtctgc ttttcgaacg ggatttgctt gataagttaa
    45661 gtggagccac accggagaaa cgactggcgc gtctggaaaa taatcaggaa attgcgctga
    45721 aacgcgacga catgaccgca gaactgctca atttgtggca tctcacgggt caggcagaca
    45781 aagcggcgga cattctcgcc acgcgtaaat tccacccgtg ggaaggcggg gaagggaagg
    45841 tcaccagtca gtttatcctc aaccagttat tacgcgcctg gcagcatctt gatgccagag
    45901 agccgcagca ggccagcgaa ctgcttcatg ccgcgctgca ttatccggag aatttaagcg
    45961 aaggccgttt accggggcaa actgataacg acatctggtt ctggcaggcg atatgcgcca
    46021 acgcgcaggg cgatgaaact gaagcgatgc gttgtttacg tctggcggcg accggcgatc
    46081 gcaccattaa catccacagt tattacaacg atcagccggt tgattatctc ttctggcaag
    46141 gaatggcgct gcgactgctg ggtgaacagc aaaccgcaca gcaactgttt agtgaaatga
    46201 aacagtgggc gcaagagatg gcgaaaacca gtattgaggc ggatttcttt gctgtttcac
    46261 aacctgacct gttgtcgctg tatggcgatt tacaacagca gcataaagaa aaatgcctga
    46321 tggtggcgat gctggcgtcc gcgggactcg gggaggttgc gcaatatgaa tctgctcgcg
    46381 ctgaattgac ggcgattaat ccggcctggc cgaaagcggc attattcatc accgtgatgc
    46441 cttttatttt taactacgtt cactaaaccc tattaactgt aatatttgag cggcacgcgc
    46501 tgccgctcat cacaaaataa ttagaataag ccgttgttaa taaaaaccct acaggaggca
    46561 ctatgcctaa aaaaatgaga acaacccgta atctattatt aatggctacg ctattaggta
    46621 gcgcactatt tgccagggct gcggataaag aaatgaccat tggtgcaata taccttgata
    46681 cctagggata ttacgctgga gtgcgtcagg gcgttcagga tgcggcaaaa gattcttcag
    46741 tacaggtaca gttaattgaa actaacgccc agggtgatat ttcgaaagaa agtacctttg
    46801 ttgataccct cgtggcgcgt aatgtcgatg ccattatttt atcggcagtg tctgaaaatg
    46861 gcagtagccg taccgttcgt cgcgccagtg aagcgggtat tccggtgatt tgctacaaca
    46921 cctgtattaa tcaaaagggg ggcgataaat atgtctcggc gtatctggtc ggcgatccgc
    46981 tggaatttgg taaaaaactg ggtaacgctg ccgccgatta ttttattgcc aataaaattg
    47041 accagccgaa aattgctgtc atcaattgcg aagcctttga agtttgtgtg cagcgacgta
    47101 aaggatttga agaagtatta aaatcccgcg ttcccggcgc gcaaattgtc gctaatcagg
    47161 aagggactgt tttagataaa gcgatttccg ttggtgaaaa actgattatc tccacgccgg
    47221 atctcaacgc cattatgggg gagtcgggcg gtgcgacact cggcgcggta aaagcggtac
    47281 gtaatcaaaa tcaggccgga aaaattgctg ttttcggttc ggatatgaca accgaaattg
    47341 ctcaggagct ggaaaacaat caggtgctga aagcggtagt ggatatttcc ggtaagaaaa
    47401 tgggcaatgc tgttttcgcg caaacattga aggttatcaa taaacaagcc gacggtgaaa
    47461 aagtgattca ggtgccaatc gatctctata ccaaaacgga agatggtaaa cagtggctgg
    47521 caacgcacgt tgatggtctg ccctaatcgc tgattcgcag aggtccctat gttcacggca
    47581 acagaggcag tcccggtagc aaaagtggtg gcaggaaata agcgttaccc cggcgtcgtt
    47641 gcgctggata acgttaactt cacgctcaat aaaggcgaag ttcgtgcgct gttaggtaaa
    47701 aacggcgcgg gcaaatcgac tctcattcga atgcttaccg gcagcgaacg cccggatagc
    47761 ggtgatatct ggattggcga gacgcgactg gaaggtgacg aagctacgtt gactcgccgt
    47821 gccgctgaac tgggggttcg tgcggtttat caggagttaa gtctggtgga gggactgacg
    47881 gtggcggaaa acctctgcct cggtcagtgg ccccgccgca acggcatgat tgattacctg
    47941 caaatggcgc aggatgccca acgttgctta caggcgctgg gcgttgacgt tagccctgaa
    48001 caacttgttt caacgctaag cccggcgcaa aagcaactgg tggaaattgc gcgggtgatg
    48061 aagggcgagc cgcgcgtggt cattcttgat gaacctacca gttcgcttgc gagtgcggaa
    48121 gttgaactgg tgatcatcgc ggtgaaaaag atgtcggcac tgggcgtggc ggtgatttat
    48181 gtcagccacc ggatggaaga aattcgccgc attgcctcct gtgccaccgt tatgcgcgat
    48241 ggtcaggtgg cgggcgatgt gatgctcgaa aacacctcca cgcatcatat tgtgtcgcta
    48301 atgctagggc gtgatcacgt tgatattgcg ccggttgccc ctcaggtaat tgtggatcag
    48361 gccgtgctgg aagtccgtgc attacgccat aagcccaagc tggaggatat cagctttacg
    48421 ttacgtcgcg gcgaagtgct tggcattgct ggcctgctgg gggcagggcg cagtgaattg
    48481 ctgaaggcga ttgttgggct ggaggagtat gaacagggcg aaattgttat caacggcgag
    48541 aaaatcacgc gccccgatta cggcgacatg ctgaaacgcg gcattggcta tacgccagaa
    48601 aaccgcaaag aagcggggat cattccctgg ttgggcgttg acgaaaatac agtgctgacc
    48661 aatcggcaaa aaatcagcgc caacggcgta ctgcaatggt ccaccatccg ccgcctgacc
    48721 gaagaggtga tgcagcggat gacggtcaag gccgccagta gcgaaacgcc catcggcacg
    48781 ctttctggcg gcaatcagca aaaagtggtg atcggtcgtt gggtctatgc cgccagccag
    48841 attttgttgc tcgatgagcc aacgcgcggc gtcgatatcg aagccaaaca gcagatttac
    48901 cgtattgtcc gtgagctggc tgccgaagga aaaagcgtgg tgtttatctc cagtgaagtg
    48961 gaggagttgc cgctggtctg tgaccgcatc ctgttattac agcacggcac gttctcgcag
    49021 gagtttcacg ctccggtcaa tgtggatgag ctgatgtccg ccattctgtc tgtgcactga
    49081 tgaattttac cgaggaacct gaagatgtct gcttcgtcat taccattgcc gcagggcaag
    49141 agcgtctcgc tcaaacaatt tgtcagtcgc catattaatg agatcggttt gctggtggtg
    49201 attgccattc tttatctggt cttctccctg aacgcgccag gctttatctc attgaataac
    49261 cagatgaacg tgctgcgcga tgccgccacc attgggattg ccgcctgggc gatgacgctg
    49321 attattatct ccggtgaaat tgatgtcagc gttgggccga tggtggcttt tgtctcggtg
    49381 tgcctggcat ttttgctgca atttgaagtt ccgctggcgg tggcgtgtct gctggtgttg
    49441 ctgttaggcg cgctgatggg aacgctcgcc ggggtgctgc gcggcgtgtt taacgtacca
    49501 agtttcgttg ccacactggg gctgtggagc gccctgcgcg gaatggggct gtttatgacc
    49561 aacgcactgc cagtgccgat tgacgaaaac gaggtgctgg actggctggg cggacaattt
    49621 ctcggtgtgc cagtatccgc gctgatcatg atggtgttgt ttgcgctgtt tgtgttcatt
    49681 agccgaaaaa ccgccttcgg gcgctcggtt tttgccgttg gcggtaatgc cacggcggcg
    49741 cagttgtgcg gtattaacgt tcgtcgagtg cgcattctta tctttaccct ttcgggatta
    49801 ttagcggcgg tgaccggcat tttgctggcg gcacgcctcg gttccggtaa cgcaggtgcc
    49861 gcaaacggtc tggagtttga cgtcatcgcc gcggtcgtgg tcggcggtac ggcactttcc
    49921 ggcggtcgcg gttccctgtt cggtacattg cttggtgtgc tggtgattac gctaatcggt
    49981 aacggtctgg tgctgctcgg tattaactcc tttttccagc aggtggtacg cggcgtcatc
    50041 atcgtggtgg cggtgctggc gaatatcttg ctgacccagc gaagcagtaa agcgaaacgc
    50101 taaaccttaa cgcccctggc gcgggtgcgg cagggatgaa aattaatcgt attgttaatg
    50161 agggatttat gaaaacgatg ctggcagctt atttaccagg aaattcaacc gtcgatctgc
    50221 gggaagttgc ggtgccgacg ccggggatta accaggtact gatcaaaatg aaatcctccg
    50281 ggatttgcgg aagcgatgtc cactatatct atcatcaaca ccgtgccacg gcggcggcac
    50341 ccgataaacc gttataccag ggatttatca acggtcatga accgtgcggg cagattgtgg
    50401 cgatggggca aggctgccgc cattttaaag agggcgaccg cgtgctggtg tatcacattt
    50461 ctggctgtgg tttttgcccg aactgccgcc gcggctttcc tatctcttgt actggcaaag
    50521 gaaaagcggc ttacggctgg cagcgtgacg gcggtcatgc cgaatatctg ctggcggaag
    50581 aaaaagatct gatcctcctg ccggatgcgc tgagctacga agatggtgcg tttatcagtt
    50641 gcggcgttgg tacagcgtat gaaggaattt tgcgcggcga agtttccggc agtgataacg
    50701 tgctggtggt cggtctgggg ccagtcggca tgatggcgat gatgctggcg aaaggtcgcg
    50761 gtgcaaaacg gatcatcggc gttgatatgc tgccggaacg tctggcgatg gcaaaacagt
    50821 taggggtgat ggatcacggc tatttagcaa ccaccgaagg tctgccgcag attatcgccg
    50881 aactcaccca cggtggcgcg gatgttgcgc tcgattgttc tggtaatgcc gcaggtcgct
    50941 tgctggcact gcgatccacc gctgactggg gacgggtggt ttacatcgga gaaaccggaa
    51001 aagtggagtt cgaggtcagc gccgatctga tgcaccatca acggcggatt atcggctcct
    51061 gggtgaccag tctgttccat atggaaaaat gcgcccatga tctgacggac tggaaactgt
    51121 ggccgcgtaa cgccattacc catcgcttct cgctggaaca ggcaggtgat gcctatgcgc
    51181 tgatggcgag cggcaaatgc gggaaagttg tgattaactt cccggattaa ggagttagcg
    51241 gatgaccatc tataccttat cccacgggtc gctaaagctg gacgtttctg accagggcgg
    51301 cgtaatcgaa ggcttctggc gcgatacgac gccgctctta cgtcctggta aaaaaagcgg
    51361 tgtggcaacc gatgcctcat gttttccgct ggtgccattt gcaaaccggg tgagcggcaa
    51421 tcgttttgtc tggcaggggc gtgagtatca actgcaaccg aatgttgagt gggatgcgca
    51481 ctatctacac ggcgatggct ggctgggcga gtggcaatgt gtctcgcaca gcgatgatag
    51541 tttgtgtctg gtgtatgaac atcgcagcgg tgtctatcac tatcgggtaa gtcaggcgtt
    51601 tcatttaacg gcggatacgc tgacggtgac gctctctgtc accaatcaag gggcagagac
    51661 gctgccattt ggtaccggct ggcatcctta ttttccgttg tcgccgcaaa cgcggattca
    51721 ggcacaggcg agcggttact ggctggagcg ggagcagtgg ctggcgggtg agttttgcga
    51781 gcagctaccg caggaactgg attttaacca gcccgcgccg ttgccgcgcc agtgggtgaa
    51841 caatggtttt gccggatgga atggtcaggc ccgcatcgag cagccgcagg aaggttatgc
    51901 catcatcatg gaaacgacgc cacctgcacc gtgttatttc atctttgttt ccgaccctgc
    51961 gtttgataaa ggatatgcgt ttgatttctt ctgtctggaa ccgatgagcc atgcgccgga
    52021 tgatcatcat cgcccggaag ggggtgatct cattgcgcta gcaccagggg aatcaacaac
    52081 ttcagagatg tcgttgcggg tagagtggct gtaatttatt aaatgccgga taaggcgttc
    52141 acgccgcatc cggcatcttt cctaacgccg atcgagagaa attgcccccg gcccggtaat
    52201 tgccagcagc aggaatgcgc cagcgatact gacattcttc cagaaattaa tcatatttgg
    52261 cccaaccgca tcgccggtca tatcccagta atggtgacca atcaccgccg tacccagcgt
    52321 gtagaaaata aacagcaccg ccagcggacg ggtgaaaaag ccaagcacga ttaatatcgc
    52381 ggcgggcact tccataacta ccgcaataat cgctgccagc atcggcattg gcgcgcccaa
    52441 cgaggccata tattggaccg taccgtcaaa gcccatcatt ttgggaaaac caaaaataat
    52501 gaaaattaag accacggcga tacgggcaat taataacaaa acggggcggg cagctccaaa
    52561 atcaaaataa cgtagtgtgt tcataaccaa tccctttgca agataatgtg atttaaacgt
    52621 aatacacact cttgcacctc gccacgcccc aggcattgag atattcgcta attatcctgg
    52681 ttacagtgat ttaagcgcga tgttctcatc tatcagcagt ttcgcatcaa acgttttgcc
    52741 gctctggatc catttgcgaa ttgggcgaat ctcacgcccc tgattcagcg tgaccgcacc
    52801 gataagcacg ccgttttgca gattaaacca aatcgccttc tgagtttccg ggttgccacg
    52861 acaaagccag tcatcgccac gcatatcgcc aataaactgt aagttatcac tgtactgatc
    52921 gctccagaac cacggcggcg gcagtagcgg tagcggtagc cctaacattg cggcagcggc
    52981 aatttgcgcc tggttattgg cgttttccca gctttcgcag cggtgtagtg caccattatc
    53041 aagacgagtg attgccacat cgccaccggc aaagatcgcg ggatcgcagg tgcggcaagc
    53101 ctcatcaatg acaatgccat tggcagtatc aaggttggcc tcgcgagcca gttgctcgtt
    53161 ggcgctgata ccaataccgt aaatcaccac atcagcctga agcgtttccc cactttgcag
    53221 cgtcagttct actttttcac catcgaccac atgttcaatg gcattattga gcagaatgcg
    53281 cacaccagcc tgctggtggc gctgtaaaag atagcgttgc acgggcggtg gtgcattacg
    53341 gcccatgacg gttgccgcca gttcaatcac tgtcacctta catctgcgct gcgtggcgct
    53401 ggcagccagt tccagaccaa tagttccggc accgacaatc acgactgacc gttcgggctg
    53461 cagaacttct cgcagtctgg cggcatcacc ggcatggcgt agggtaaagc agcgttctcc
    53521 cagtgcatca agcaacggca gcggtcgagc tgccgcgccg gttgctataa aaagctgatc
    53581 ccagtgccag ctttcgccgt tggttaacac taactctcgt gtgtcgcgac ccagcgtttt
    53641 gatggttaca ccggaatgca gatgaacatt gttttcctgc caccagttag cgggtaacac
    53701 ctgctgtaac tgcggggaat cttccagcaa catggatttc gagagtggag gtcgttcata
    53761 aggaagatgt cgctcatcgg aaaacagatg cagctcaccg gtgaaccctt gctggcgtag
    53821 cgaggccgca gccattgccg ccgcttgccc gccaccgaca ataatgatgg ttttttcttt
    53881 catcgtgctt ccttaaagat ccagcccagc cgctacgtgg cgaatgccgc gaatcgctaa
    53941 acccgcatca gcgttgatca tcacaccgct taatgcgcga ttattgcgcc gcgatgccaa
    54001 catcacatac ggccccgtaa aatccgccgg ttgcgggaaa aattgcagcg gtaaaatggc
    54061 ggcaattttc tccggcgtca gagactgcat tatcgaggtt tcactttgcc cgagcgcctg
    54121 tgggccgcgc aggtcgctgg ccataccaca cgggccgacg ccattcaccc gcactttcgg
    54181 tgccagttca taagccagtt ggcgaataag tccggttgcg gcatgtttac tggcggtgta
    54241 cagcgggcca ccgccgccag gataccaggc ggcatttgac agtgtgaaaa tcatgctgcc
    54301 ttcactggcg attaacgccg gagcgcaggc ttttgcgccc agcaggtaac cgagaacatt
    54361 gacgttaaac agctcgtgga agccggtttc gagcgtctct gcgggagtat taaccagtga
    54421 ggcattgtga tcccagatgc ctgcattgcc gataaaacaa tccagcttgc cggaacgagt
    54481 caggatctga tcgaccgcgc gttgataatc ggcataacag gtcacgttac cttccaccgc
    54541 cagaatatgt tcgccaaatc gctgacgcag actggcgact tttgccgccg acagttccag
    54601 cgtggcaacc tgcgcgcctt cttcgataaa tcgctcgacc agcgccagcc ctaatcccga
    54661 tccgccgccg gtaataaaaa tggactcgtt atgcagatcg ctcatggctg cgcctccggt
    54721 aagtcgatga aaatgtcacc accttcaacg tgtactggat aagtggtgag cggatcggtg
    54781 gcgggcaggc ataacgcttt ccccgttttc aggcaaaaac tggcggcgtg tagcgggcac
    54841 tccaccgtgg cgtcatcttc cagataccct tctgacattg acgcattacc atggctgcaa
    54901 cgatcgttaa tggcataaaa ctcgccgcca acgttgaaca gggcgatgac gggcgaggta
    54961 tcgatccgga gagcctcacc ctccggcaca tccgcaacgg gacacgcata aattcgattc
    55021 atcagaacag tacactcagg ttatgggaag tgattaccgc ttgatccagg acgatatccc
    55081 gttccagcaa gcgccagttg tcatcttcca gacggcgaac tttgtcgaaa cgcgttccaa
    55141 catagaatgt ttcatcgcgc tctttttgtg cccgataaag cagataattt acccgcacag
    55201 caaatacgtt tgggatgtcg gtttcgctta tctggcagtt gctgattaag tgacgggtgc
    55261 gtgacggcgg ctcttctgcc caggccatgc ccgtttccag acgggcgatt cgccgctcca
    55321 gctggtcttt ggtgtcatta aaaatccagg ttgtcggtgg ctggacgcct ttgcggcggt
    55381 cgcgtgtttg cgcgttaact gtggtgcgca tggtgtaacg aatctcttcg tcgagctgcg
    55441 ccagccagtc acgaaatttc cagtcgtcca gtaagctggc ttcgtgaaag agaaactggc
    55501 taatgcggtg atgtaactct agtgaaactt gcgcactcat ttcatcacct cctgctggta
    55561 agcggcggtt ttatcgagca cttcctgcca gctttcgcta ctcagaagat cagcccagcg
    55621 ttggtacatt ccacgagcgg cagtttctga aaagatatag ttagtaatgc caggaatgcc
    55681 gtcgtcgcgg cgcttttcct gaccaagccc catttccaga cacagtttgc tgttgcgggc
    55741 gcggtggcct ttaagcaatt tctggatttc acaccagttc tccgagtcat cctgctcgag
    55801 aaaaccagca ggaccaaaag cacgagtggc gctgttttca aaagcggctt taacttcatc
    55861 ggaggcggct ttgtcagtaa tacagaacgc ccacacttca acttgatcag ggccgcgcgg
    55921 atgccagacg cggagtgtgg cagtgccgtt tagccatgaa agcgtgggga atatattgtt
    55981 atgacccgcc aggcgcaggg cgcgaacttc acctaaacgt tgttctgctt cggcataggt
    56041 ttcgcggtaa tagcttgaaa ctgcgccatc gacccagaca ttagcatccg gtttttcagt
    56101 aaagaagaaa ccgctaccgt gaccgtcctg accaaattgc agcgcatctt tggcggtttc
    56161 ccacaccggg cgggcggttt gtccatcacc gaggcgctta tcgctgccat catctttcgc
    56221 ccctaatacc tgaacggcag aagcatggct gaacagagca tgatactggt cactggcgaa
    56281 ctgctctgcc gggaatttcc agttacagtt gatcacccac ttttgtacgc cgccgacaat
    56341 ttcggtgccg ccttcgcgac gatccagcat gccatccaga taccaggcaa tgtcacccag
    56401 gtaatcacgc aggcccggtg cgctggtatc ccagttgcca aaaatcagcc ctttataact
    56461 ctccacacaa ggaacttcgt ttagtcccca gtgggattta cacaaccctt gtgggtaggc
    56521 gcgaggttcc agcggtacat cgatcaactc gccgttaatg ccataagacc agccgtgata
    56581 cggacaggta aaggcgcgag tgttgccgca atctgcataa ctcacacgca tggcccggtg
    56641 gcggcattgg ttgagaaacg ccttaatgct gccgtctttc tgacgcacta cgacaaccgc
    56701 atcttccccc atgtaggtgt taaagaaatc accaggtttt gggatctggc tttcgtgggc
    56761 gagaaataac cagcaacggc cgaaaatacg ttcaagctcc agttggtaaa tatctgggtc
    56821 ggtataaata cgcggagtga cccgaccatt ttgggtatca atcagttggt aaatgttcaa
    56881 atctgagggt gtggtcatat tttttaatcc tgaaagataa gatgtattga ccttctattt
    56941 ttgtgccata aggatgttgc tggttggttg ctaatatgtg aaatagttaa tttctaccag
    57001 ctgagacgtt ttttcgataa caaggggaag gtgatggaac tacggcattt acgctatttc
    57061 gtcgcagtgg cgcaggcact gaactttacc cgtgcggcgg aaaaactgca tacctcacag
    57121 ccttcgttaa gcagccagat ccgcgatctt gaaaactgtg tcggtgttcc gttactggtg
    57181 agggataagc gcaaagtcgc gttgacggcg gcgggagaat gttttctcca ggacgcgctg
    57241 gcaatcctcg aacaagcgga aaatgccaaa ttacgggcgc ggaaaattgt tcaggaagac
    57301 agacaattaa ccattggctt tgtgccatcg gcggaagtga atttactgcc aaaagtatta
    57361 ccgatgtttc gtctcagaca gccagacacc ttgattgagc tggtgagttt aatcaccacg
    57421 caacaggagg aaaaaattcg ccgtggtgaa ctcgacgtcg gcttaatgcg ccatcctgtt
    57481 tatagcccgg agattgatta tctggagctt tttgacgaac cattagtggt tgtgttaccg
    57541 gttgatcacc ctttagcaca tgaaaaagag atcaccgccg cgcaactgga tggcgtgaat
    57601 ttcgtcagta ccgatccggt gtattccggt tcgcttgcgc cgatcgttaa agcgtggttt
    57661 gcgcaagaaa acagccagcc aaatatcgtc caggtggcaa cgaatattct ggtgaccatg
    57721 aatctggtgg gcatggggct gggcgtcact ttgatacccg gttatatgaa taattttaat
    57781 accggacagg ttgtttttcg tcccattgcc ggaaatgtgc cttccattgc tttactgatg
    57841 gcgtggaaga aaggggagat gaagccggca ttacgcgatt tcatcgccat tgtgcaggaa
    57901 cgtttggcaa gcgtaacggc ataattacgc attatttttg caacatgcgt cgcggatctc
    57961 agttttttag tgcttgtgaa ctggcgttac agacatcggg aagcaggctc gaatattctt
    58021 atttttcaac ttgcaccaac attcgattaa ccccagacta acgcctcctg acgggaggga
    58081 ctcatggttt tgcaatccac gcgctggttg gcgctcggct atttcacata cttttttagt
    58141 tacggcattt ttctaccttt ctggagcgtc tggcttaaag ggattggttt aacgccagaa
    58201 accatcggcc tgttattggg ggcaggtctg gttgcccgtt tcctcgggag tttgctcatc
    58261 gcgccccgcg tcagcgatcc ttcccgcctg atttccgcct tgcgcgtgct ggcactgctg
    58321 acacttctct ttgctgtcgc cttctgggcg ggggcgcacg tagcgtggct gatgctggtg
    58381 atgattggct ttaacctctt tttctcacca ctggtaccgt tgaccgatgc actggcgaat
    58441 acgtggcaaa agcagttccc gcttgattac ggcaaagtgc gactgtgggg ctcggtggcg
    58501 tttgtcattg gctcggcgct gacgggcaaa ctggtcacta tgtttgatta tcgggtgatc
    58561 ctcgcgctgt tgacgttggg cgtggcatcc atgctgctcg gctttctcat ccgtccgacg
    58621 attcagccac aaggggcaag tcgccagcag gagagcaccg gttggtctgc gtggttggcg
    58681 ctggttcgcc agaactggcg ctttctggcc tgcgtttgtt tattgcaggg ggcacatgcg
    58741 gcctattacg gttttagcgc catttactgg caggcagctg gctactcggc ctcggcggtg
    58801 gggtatttgt ggtcgctggg cgtggtggcg gaagtcatta tctttgcgct gagtaataaa
    58861 cttttccgcc gttgtagtgc acgcgatatg ctgttgatct cggcgatttg cggcgtagtg
    58921 cgctggggca ttatgggagc aactacggcg ttgccgtggt tgatagtggt gcaaattctg
    58981 cattgcggca ccttcacggt ctgccacctg gccgccatgc gttatattgc tgctcgccag
    59041 ggtagcgaag tcatccgttt acaggcggtt tactctgccg tcgcgatggg cggcagtatc
    59101 gctatcatga ccgttttcgc cggtttcctg tatcaatatc tgggccacgg cgtgttctgg
    59161 gtaatggcgc tggtggcgct gccggcaatg tttttgcgcc cgaaagttgt tccctcatgc
    59221 tgattcgagc attttgcgga tctgctcctg ttgatgtgtt gtcagcgtca ggtccgtata
    59281 gatcagcggt ggtgaaaaga gcggtaatgg catggtataa ggcgcaataa tcagtgccgc
    59341 gccacgcgga gcgcctgtct gcaaaaatgc ctttaccgac atatgcttaa tgttgagcgg
    59401 cagtaacgtt agttcgcgta actgctgctc aatctgcgct tctcgctcgc tatcattccc
    59461 ggtcagtaga ataatctgtt tttcatgcag atcgttttcc tgcattagcc aggcaccgaa
    59521 aatcaccgcg accagaccac tttcctcatc agaaagatgg acgccgtatt cactttcaaa
    59581 tccggccagc gccgcgcggg tggtgcgcac caggcgtggg tacagtctgg cgaactcttc
    59641 cggcagggta ttatcaatac cgatggcaaa caaactgcga tttaacgcct gggcgaggtg
    59701 ggtataaagc tgatcacata acccctgttc atcataaaaa cgaacatttc ccagctcacg
    59761 aaaatggttt accagacgtt tgatagactg acgcaattgt ctgtctcgct gatgcgcatc
    59821 ccgcagtgga tcgggaaccc gcaacatgga aaaaagtagt gccataaaca gtggctcatc
    59881 aggcggtaca ggctggagcg cccgacgctg ccagtggcgt ccaatttcct gcgctacctg
    59941 aaattcaagg caggattccg cccagcgacg ttggagcgga ttaaactgag gcgtaattcc
    60001 agcgtggtgc tgcagcaaac aatattgcag atacagacac aggaaatgaa tatcgcgcga
    60061 ttcaaagcgt ttttgcagcc gtcgggagca gagattcact aacgcttgta gattggtatc
    60121 gtcgtaaaag ttacgcgcaa ttccgcgccg cttcagttca ctcttcaggg cgggggtgaa
    60181 ttggctggta ataaatgacg gacacagacg gaaaccacgt cgtagccagt gaaataaaca
    60241 caaacgttgg ttaagcactg taccttcaac ccggtagcta ccgtcataac cagtcgtcag
    60301 tgtgagttga tgatagcgca ggatctcccg tcctgtttcg ctgatatcaa gactggcaat
    60361 atcatcatcc acaccattaa gctcgctgaa ggttgccatg gtggcggtca accccggctg
    60421 aaagagcgtc agcaagatct ggcagcggcg ctggggagcc gaaaggacag atggtggagc
    60481 aagcgtaggc atcatctgta gatctcccat aagcataatt gataagaata gctaaagttt
    60541 gctcagggaa atggcgaagg gaatgctaat catcagaaat gttggcaagc gtcacagaat
    60601 ttttctcatc ttaaactgtt ctgaccagtg gattagcatg tcttaaggtg gttttgtgaa
    60661 tgattatcgt gctagcgatc atgggttagt tctgcggatt caacgcgcta aggataaagt
    60721 ccaccatctg ttcgcgataa acatcgccgt ggcgatcgga aaaagcatca atgtgttcgc
    60781 catccgggat taggatcaac cgttttggct cttttgccag gctatacaac ttttcgctgt
    60841 gctgccatgg gataacgtga tcagctttac cgtgaatgag taaaagcggg atcgggctga
    60901 cgctggcgat ataattttcg ccgctgtaac tctcatcaag taagtagcca ctgccgggga
    60961 tcatctggtt ggcgatggtt gcataagagg caaatgtgga gtcgaggatc accgcacgta
    61021 tgccttcacg atcaccctga ccaataacag caagaatatt cgccccgcca atactctgcc
    61081 cgaacagcac cagccgttgt gggtttacat cactgcgatg gcgcaccaca ttgatggcgc
    61141 tttgcgtatc gtccagcaat ccggcctggg acggcgtgcc ttttgattta ccaaacccgc
    61201 gataatcaaa cataaaaacg ttgaaattac gctcgggtaa ccaactgacc agcggccagt
    61261 gggcggacat atttccggca ttgccgtgag catgaatgat ggttgcgatg gcgttgtcag
    61321 cagggcccgt cgaagaagga ataaaccagc cttgcagacg agtaccgtcc ttagccgtaa
    61381 attcgacgga ttccgccgac caggggtcgg gaccgtaaat tttatcgtca ggataataga
    61441 agaagttgat ggcaacgcgc ggcactaaat aaacgcagaa ggcaacaaca aagagaataa
    61501 acagaatttt gggaacgcgt ttgttcactg gcagtgccat aacctttcct tgcgacttct
    61561 ttatcatcca ttaatcgggg aaagatggtg ggagagtgac gaaagaaagt aaagcggtga
    61621 ggaagtattt cagaatagcg cctgaaaggc gagggcgggt gagtgacatc acccgcctga
    61681 gtcattaacg cttcagagcg tcgcttaact cgtcacgcat gttcgccagc atggctttca
    61741 caacacgtgg gttaccagca acgatgttac cggtcagcat atagttatga ccaccggtga
    61801 agtcgctgac gatgccgccc gcttcacgaa ccagcagctc gcctgccgcg aaatcccacg
    61861 gacgcagacc gatttcaaag aaaccgtcaa cacgacccgc agcgacgtaa gccagatcca
    61921 gcgccgcaga accggtacga cggaagtctg cacattcgtt gaacagtttg ccgacgatgt
    61981 tgatgtaggt agtggcgtac tgttttgctt tgaacgggaa gccggtcgcc agaatggtac
    62041 cgtcgagatc gcgagcggtg ctaccgcgca gacggtagcc gttcagctgt gcgccctgac
    62101 cgcgagtggc ggtgaacagt tcgttacgca taggatcgta taccacagca acttcggtgc
    62161 ggcctttgat acgaacggcg atagataccg cgaagtgcgg cagacgtttg ataaagttgg
    62221 tagtaccatc cagtggatcg ataacccatt gaacatcctg atcagtacct tcaagttcac
    62281 cgctttcttc ggtgatgatg gtgtgctgtg ggtaagattt acgaatcgtg tcgataatca
    62341 ccgcttcggc agctttatct acgttggtca cgaaatcgtt actgcctttc tggctcgctt
    62401 ctacagcgtc cggggtttca tagtttttgg caattaaatt acccgccttg cgcgctgcgc
    62461 gcacggcgat gttcagcatc ggatgcatcg gtctctctca ctggatgtta aagaacggga
    62521 aaacggcgca gagtatagca gcgagatcgg catctgtctt cgttttatga taagatgcgt
    62581 gaataatctt tagatgaaga aagacaatgc tgcaaaatat tcgaattgtg ctggtggaga
    62641 cgtcacacac cggcaatatg ggttctgttg cccgtgccat gaaaacaatg ggattaacca
    62701 atctgtggct ggttaatcca ctggtgaaac ccgactccca ggcgattgcc ctggcagcag
    62761 gggccagcga tgtgattggt aatgctcaca tcgtcgatac gctcgacgaa gcgttagccg
    62821 gttgtagcct ggtggtgggc accagcgcac gctcccgcac gctgccatgg ccgatgctcg
    62881 acccgcgcga atgcggcctg aaaagcgtcg ctgaagcggc aaataccccg gtggcgctgg
    62941 tgtttggtcg cgagcgcgtc ggcttgacca atgaagagtt gcagaaatgc cattatcatg
    63001 tcgcgattgc ggctaacccg gaatacagct cgctgaacct ggcgatggcg gttcaggtta
    63061 ttgcctatga agtgcgcatg gcctggctgg caactcagga aaacggcgag caggtcgagc
    63121 atgaagagac gccgtatccg ctggtcgatg atctggagcg tttttatggt catctggaac
    63181 aaacgctgct ggcaaccggt tttatccgtg aaaaccatcc ggggcaggtg atgaataaat
    63241 tgcgccgtct gtttagccgt gcgcgcccgg aaagccagga attgaatatc ctgcgcggga
    63301 ttctggcttc tattgagcag cagaataaag gtaataaggc ctgagggcta agccagataa
    63361 atgcacggac ggtcccctcg ccccttcggg gagagggtta gggtgagggg aagaggccag
    63421 cgctaatgcg aataattgcc ctcaacccgc ccatggtctg gaaggacaac aggtacaaac
    63481 gccacgataa aaaaatggca ctgaaggtta aatacccgac taaatcagtc aagtaaatag
    63541 ttgaccaatt tactcgggaa tgtcagactt gaccctgcta tgcaataccc ccacttttac
    63601 aataaaaaac cccgggcagg ggcgagtttg aggtgaagta agacatgaga ctgacatcta
    63661 aagggcgcta tgccgtgacc gcaatgcttg acgttgcgct caactctgaa gcgggcccgg
    63721 taccgttggc tgatatttcc gaacgtcagg gaatttccct ttcttatctg gaacaactgt
    63781 tttcccgtct gcgtaaaaat ggtctggttt ccagcgtacg tggaccaggc ggtggttatc
    63841 tgttaggcaa agatgccagc agcatcgccg ttggcgaagt gattagcgcc gttgacgaat
    63901 ctgtagatgc cacccgttgt cagggtaaag gcggctgcca gggcggcgat aaatgcctga
    63961 cccacgcgct gtggcgtgat ttgagcgacc gtctcaccgg ttttctcaac aacattactt
    64021 taggcgaact ggttaataac caggaagtgc tggatgtgtc tggtcgtcag catactcacg
    64081 acgcgccacg cacccgcata caagacgcga tcgacgttaa gttacgcgct taatttaacc
    64141 gtcctgtttc gtgttgcatc tgcgttgact gcacatactc actccggtca cttacttatg
    64201 taagctcccg gagattcgta agtttgtcaa cttgctgaaa cccgaaacag ttaggttaaa
    64261 gataacaaga tattagaatc aggccggggc gtatcgcgct ccgtaaacac ggtcgtacat
    64321 ccagccggta gcctggttcc ttgcattgag tgatgtacgg agtttataga gcaatgaaat
    64381 taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt gccgagaaaa
    64441 tgatgcagtt tatgacgatg gacggaacct ttggtaaccc ggcctctcgt tctcaccgtt
    64501 tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc gatctggtcg
    64561 gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac aacctggcga
    64621 tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc agcaaaaccg
    64681 aacacaaagc ggtactggat acctgccgtc agctggagcg cgaaggtttt gaagtcacct
    64741 acctggcacc gcagcgtaac ggcattatcg acctgaaaga acttgaagca gcgatgcgtg
    64801 acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg gtgcaggata
    64861 tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt gatgcaaccc
    64921 agagcgtggg caaactgcct atcgacctga gccagttgaa agttgacctg atgtctttct
    64981 ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtacgt cgtaaaccgc
    65041 gcgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg cgttccggca
    65101 ctctgcctgt tcaccagatc gtcggaatgg gcgaggccta tcgcatcgca aaagaagaga
    65161 tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac ggcatcaaag
    65221 atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac attctcaacg
    65281 tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac ctcgcagttt
    65341 cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg cgcgcgctgg
    65401 ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt tttactactg
    65461 aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt ctgcgtgacc
    65521 tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc gaatgggctc
    65581 atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa aaagttatcg
    65641 accattacga gaatccgcgt aacgtgggtt cctttgacaa caacgacgag aacgtcggca
    65701 gcggcatggt gggtgcaccg gcctgtggcg acgtgatgaa gttgcagatt aaagtcaacg
    65761 atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttct gctatcgctt
    65821 ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg caggcgatca
    65881 aaaataccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac tgttctattc
    65941 tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt gaagcaaaat
    66001 aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc gcgagtaaat
    66061 acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag aacctccgga
    66121 tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga agacatcgtg
    66181 tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt tctggacggt
    66241 acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac caacccgaac
    66301 gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata ccgataaccc
    66361 caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc gcggcggatc
    66421 gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc cgttatcaac
    66481 tcgataccca ggcgctgagc ctgcgttttc aggatctaca acgtcagtat catcctgata
    66541 aattcgccag cggaagccag gcggaacaac tcgccgccgt acagcaatct gcaaccatta
    66601 accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg ctttctttac
    66661 acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc ctgatggaac
    66721 agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa gcgcggctgg
    66781 aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg atggttgaac
    66841 agttagacaa cgaggcgtgg gacgcggcgg cggataccgt gcgtaagctg cgttttctcg
    66901 ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt taatttctgg
    66961 aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc gccgcatcag
    67021 cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc gacagtgcgc
    67081 agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc atctgttgtt
    67141 cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc agcgcttgac
    67201 accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc tgatatccag
    67261 caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct gccgatgatt
    67321 gaaacggcgg cggggctgct gaacccggtg cgcgtttctg cggacatcct caaagcactg
    67381 gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat caccgttccg
    67441 gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct ggcgggcctt
    67501 cacgtcctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg gctggattcc
    67561 ggtcaggaag gcgtgatcgc cgtttatgac ctcggcggcg ggacgtttga tatttccatt
    67621 ctgcgcttaa gtcgcggtgt gtttgaagtg ctggcgaccg gcggtgattc cgcgctcggt
    67681 ggtgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcggg cattcctgat
    67741 cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc caaaatcgcg
    67801 ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga aatcagccgt
    67861 gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc ttgtcgtcgc
    67921 gcgcttaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat ggtgggcggt
    67981 tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg tccaccgctg
    68041 acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc ggatattctg
    68101 gtgggtaaca agccagacag cgaaatgctg ttgcttgatg tgatcccact gtcgctgggc
    68161 ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac tattccggtg
    68221 gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc tatccatgta
    68281 atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt tgcgctgcgt
    68341 ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca ggtcgatgcc
    68401 gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc gtctattcag
    68461 gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa agactcaatg
    68521 agctatgccg agcaggacgt aaaagcccga atgctggcag aacaaaaagt agaagcggcg
    68581 cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt aagcgccgca
    68641 gaacgtcagg tcattgacga tgctgccgct cacctgagtg aagtggcgca gggcgatgac
    68701 gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga tttcgccgct
    68761 cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga cgaggtttaa
    68821 tatgccaaag attgttattt tgcctcatca ggatctctgc cctgatggcg ctgttctgga
    68881 agctaatagc ggtgaaacca ttctcgacgc agctctgcgt aacggtatcg agattgaaca
    68941 cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg aaggttttga
    69001 ctcgctgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct ggggactgga
    69061 gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag tggtcgaaat
    69121 cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat gggacttaaa
    69181 tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga tcttgatccg
    69241 aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga tttcgacgac
    69301 gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg gctggacgag
    69361 gccgaataat ttccgcgtca tgcttcacgc cgcagatgcg ttggctgcgc tcgttcaccc
    69421 cagtcacata gttatctatg ctcctggggc ttcactcact tgccgccttc ctgcaacgcg
    69481 aatcatttag cggaaaaatc ctggggctgc caactggcgg cccttttaca aagaaggata
    69541 actaaaatga cagaagcgat gaagattacc ctctctaccc aacctgccga cgcgcgctgg
    69601 ggagaaaaag caacttacag cattaataat gacggcatta ccctgcattt gaacggggca
    69661 gacgatctgg ggctgatcca gcgtgcggcg cgcaagattg acggtctggg catcaagcat
    69721 gttcagttaa gcggtgaagg ctgggatgcg gatcgctgct gggcattctg gcaaggttac
    69781 aaagccccga aaggcacgcg taaagtggag tggccggatc tggacgatgc ccagcgccag
    69841 gaactggata accgcctgat gatcatcgac tgggtgcgtg acaccatcaa cgcaccggca
    69901 gaagaattgg gaccatcgca actggcacag cgtgctgttg atctgatcag caacgtcgcg
    69961 ggcgatcgtg tgacttatcg gatcaccaaa ggcgaagatc tgcgtgagca aggttatatg
    70021 gggctgcaca ccgtcgggcg cggttcagaa cgttctccgg tattgctggc gctggattac
    70081 aacccaactg gcgataaaga agcaccggtg tacgcgtgcc tggtaggtaa aggtatcact
    70141 tttgactccg gcggctacag catcaaacag actgcgttta tggactcgat gaagtcggac
    70201 atgggcggcg cggcaacggt taccggggcg ctggcatttg ccattacgcg cggactgaac
    70261 aagcgcgtga agctgttcct ctgctgtgcg gataacctga ttagcggcaa tgcgttcaag
    70321 ctgggcgata tcatcaccta tcgcaacggt aaaaaagttg aagtgatgaa cactgatgcc
    70381 gaagggcgtc tggtgcttgc cgatggtctg attgatgcca gtgcgcagaa accggaaatg
    70441 atcattgatg cggcgaccct caccggggcg gcgaaaactg cgctgggtaa tgattatcac
    70501 gcgctgttca gttttgacga tgcgcttgcc ggtcgtttgc tggcgagtgc ctcacaagag
    70561 aacgaaccat tctggcgtct gccgctggcg gaattccacc gcagccagct gccgtctaac
    70621 tttgccgaac tgaacaatac cggaagcgcg gcgtatccgg caggcgcgag cacggcggcg
    70681 ggcttcctgt cgcactttgt tgagaactat cagcaaggct ggctgcatat cgactgctcg
    70741 gcgacttacc gtaaagcgcc ggttgaacag tggtctgcgg gcgctacggg acttggtgtg
    70801 cgcacgatag ctaatctgtt aacggcgtaa actaatatgc cggatgcggt gtaaacgcct
    70861 tatccggcct acaaattctg caaattcaac aaattgcgaa tcccttgtag gcctgataag
    70921 cgtagcgcat caggcatttg cgccccgaac gttgaatgaa ttgtggttca tatgtccgaa
    70981 acaaaaaacg aacttgaaga cctgctggaa aaagcagcaa ccgaaccggc gcaccgcccg
    71041 gcctttttcc gtactctact ggaatccacc gtctgggtgc ctggtacggc ggcgcagggc
    71101 gaggctgtgg ttgaagatag tgcgcttgat ttacagcact gggaaaaaga agacggcacc
    71161 agcgtcattc cttttttcac ctcgttagaa gcgcttcaac aggcggttga agacgaacag
    71221 gcatttgtcg taatgcccgt tcgcacattg tttgagatga cccttggcga aacgctcttc
    71281 cttaatgcca aactgccgac cggtaaagaa tttatgccgc gtgaaatcag tttgttgatt
    71341 ggtggagagg gaaatccgct gagcagccag gaaatcctgg aaggcggtga atcgctgata
    71401 ttatcggaag tcgcagagcc gccagcacaa atgattgatt cactcaccac cttatttaaa
    71461 accattaagc cggtgaagcg tgcttttatt tgttcaatta aagagaacga agaggcacag
    71521 cctaatttac ttattggcat tgaagccgat ggtgatatcg aagaaattat tcaggcgacg
    71581 ggaagtgtag cgaccgatac attacctggc gatgaaccaa tcgatatttg tcaggtgaaa
    71641 aaaggggaaa aaggaattag ccactttatt accgaacata ttgcgccatt ctatgaacgt
    71701 cgctggggtg gttttttgcg tgactttaaa cagaaccgga taatctaaaa tatgccgccc
    71761 taaagggcgg cattcgggct tacttgctgc tctgaaggtt gttaaccacc agtaaattgt
    71821 cgccggagag actccggtta ttatttactt gggaaaacag cgcaccagaa acaataagta
    71881 cgatacagaa gcaccgtatc ttcattgtgc ggtccatgcg aagaccgcat cacgacagca
    71941 ccactggcga tattgccgcg atagcaagcg ctgagacgcg cttgctctaa cggtcgcgga
    72001 acatcctttc cttaagccag cgcccgcttg cgagacggcg gaaagggccg cagtggataa
    72061 tacactaaca gaacaataat aactctcttt tgcttgacaa aaagagagtt actggcgagt
    72121 attgtctccg ctgagacgcg cttgcattac tacggtaata caacctgctg gaaaggccgc
    72181 gaaccagacc agcaataaaa aaccgccaaa ttcggcggtt ttttattgcc tgtaaaatac
    72241 ttatttcact ggctcaaccg gtaaatctgc ccgcgcgccc cattcactcc atgcgccgtc
    72301 gtacagtttc acgtttggca catccagcgt cgcgagtgct aacaaaacca cggctgccgt
    72361 tacaccagag ccgcagctga cgataattgg tttgtcgtag ctgacgccgc gaccaaaaaa
    72421 tatcgcatcc agttcatcgg tcgtttttag ttcgccttcg cgcaccagtt ccgtccacgg
    72481 aacattcagt gctccgggaa tatgtccgcg acgtaaacct gggcgaggtt catcaacttc
    72541 tgcgttaaaa cgtgcagccg ggcgggcatc aataatttgc gccgtatttt catggcttgc
    72601 caataataca tcggttactt tcaccacggc ttcaggatta aacgcggcgt taaactctcc
    72661 ttccggcagc tctactgcac cttcttctaa cagcagatca tcgcgctgcc agcctgcaag
    72721 tccacccccc agaatcgaca ctttctctac accaaaggtg cgcagcatcc accatgctcg
    72781 tggggctgag aaaagattac cttcgtcata gacaatcagg tgcttatcct ggttaacgcc
    72841 taattcacgc atcgccacgg cgaacgtttc cgggcgcggc agcatgtgcg gaagcgggga
    72901 agtgtgatca gaaagcgctt cgatatcaaa aaacactgcg ccgggaatat gtccattcag
    72961 atactcctga gcaacgttac gatcttcctg tccaggcgac gccatgcggg catcaataat
    73021 ctgaatttcc gggtcatcaa tatgttcggc gagccagtcg gctcctacaa accatgtcgt
    73081 ggacataggc atctccattt ttgccggggt taggctaaat tgtcggatgt ttagtgaaaa
    73141 gcgacaagcg aaagaggctg atttggctgt ctgagccaca attctcacta aaattcggat
    73201 tgtatgggcg tgatactgaa ttacataacg taaattctca cgcataataa ttattcgacg
    73261 ttgctaacgg gccattgtgg cagggatgaa aaatgaaaaa gttacgcgta gccgcctgca
    73321 tgctaatgct ggcgctggca gggtgcgaca acaacgataa cgcgccaaca gcggtgaaaa
    73381 aagatgcgcc ttctgaagtt actaaagcgg cctcttcaga aaacgcgagt tcagcaaaac
    73441 tctccgtgcc ggagagacaa aaactggccc aacagagtgc cggtaaggtg ctgacattgc
    73501 tggatctctc tgaagtccaa cttgatggtg cagccacgct ggtgctgacg ttctccatcc
    73561 ctctcgaccc ggatcaggat ttctcacgcg ttattcatgt cgtcgataaa aaaagcggca
    73621 aagtggatgg tgcctgggag ctgtcagata atcttaaaga gttacgttta cgccacctcg
    73681 aaccgaaacg tgatttgatc gttactattg gcaaggaggt caaagcactc aacaacgcaa
    73741 ccttcagtaa agattacgaa aaaactataa ctacccgcga catccaaccc agcgtcggtt
    73801 ttgccagccg tggttcgctg ctgcctggca aagtcgttga agggctgccg gtaatggcgc
    73861 tcaacgttaa taatgtcgat gttaacttct tccgcgttaa gccagaatct ctgccagcat
    73921 tcattagcca atgggaatac cgcaattcgc tggcgaactg gcagtcagac aaactgctgc
    73981 agatggcgga tctggtctac accggacggt ttgatctcaa tcctgcgcgt aacacccgtg
    74041 aaaaattatt gctgccgctg ggcgatatca aaccgcttca gcaggcgggc gtgtatctgg
    74101 ctgtgatgaa tcaggctgga cgttacgatt acagtaatcc cgcgacgctg tttacgttaa
    74161 gtgatatcgg cgtttcagct caccgttatc acaatcgtct ggatatcttt acccaaagtc
    74221 tggaaaacgg cgcggcccag caaggaattg aagtctcttt attaaatgag aaagggcaga
    74281 ctctgactca ggcaaccagt gacgctcagg ggcatgtgca gctggaaaat gataaaaacg
    74341 cggcattact gttggcgcgt aaagacggtc agacaacgct actcgattta aaacttccgg
    74401 cgctggactt agcagaattt aacattgctg gcgcgccagg ctatagcaaa cagtttttca
    74461 tgtttggccc acgcgatctt tatcgcccag gtgaaacggt aatcctcaat ggtttgctgc
    74521 gtgatgcaga cggtaaagcg ttgcccaatc aacccatcaa gttagacgtg attaaacccg
    74581 atgggcaggt actcaggagc gtcgttagtc agccggagaa tggcctctac cactttaccg
    74641 ggccactcga tagcaatgcg gcaaccggta tgtggcatat tcgcgctaac acgggcgata
    74701 accagtatcg gatgtgggat ttccacgtcg aagattttat gccagagcgc atggtgctga
    74761 atctgaccgg tgagaaaacc ccgctaacgc cgaaagatga agtgaaattc tccgtggtgg
    74821 ggtactacct gtatggtgca cctgctaatg gtaatacttt gcaagggcaa cttttcctgc
    74881 gcccactgcg tgaagctgtg tcagccttac ctggttttga attcggcgat atagctgccg
    74941 aaaatctttc ccgcacgctg gatgaagttc agttgacgct ggatgataaa gggcgcggcg
    75001 aagtttctac agaaagccag tggaaggaaa cgcattcccc attacaggtt attttccagg
    75061 gtagtttgct ggaatcgggc ggtcgcccgg tgacgcgccg cgctgagcag gctatctggc
    75121 ctgccgatgc attgccgggg atccgtccgc agttcgcctc gaaatcggtt tacgattatc
    75181 gtactgacag cacggtgaaa cagcccattg ttgatgaagg cagtaacgcc gcttttgaca
    75241 tcgtttatag cgatgcgcaa ggcgtgaaaa aagccgtgtc gggcttgcag gtgcgcctga
    75301 ttcgcgaacg ccgcgattac tactggaact ggtcagaaga tgaaggctgg cagtcacagt
    75361 ttgatcaaaa agatctgatc gaaaatgaac aaactctgga tctgaaagcg gacgaaaccg
    75421 gcaaggtcag ttttccggta gagtggggcg cttatcgtct ggaagtcaaa gcgccgaatg
    75481 aagcggtcag tagtgttcgt ttctgggctg gctatagctg gcaggacaac agcgacggta
    75541 gcggcgcagt gcgacccgac cgtgtcacgc tgaaactgga taaagccagt tatcgccctg
    75601 gcgacaccat taagttgcat atcgccgcgc caacggcggg taaaggttat gcgatggtcg
    75661 agtccagtga agggccgctg tggtggcaag agattgatgt tcgggctcaa gggctggatc
    75721 tgacgattcc ggtcgataaa acctggaatc gtcatgatct gtatttaagt acgctggtgg
    75781 tacgtcctgg cgataaatct cgctccgcga cgccaaaacg cgcggttggt gtgttgcatc
    75841 tgccgcttgg cgatgaaaac cgtcgcctcg atctggcgct ggaaacacca gcaaaaatgc
    75901 gtcccaatca accattaacc gtgaaaatta aagccagcac taaaaatggc gagaagccta
    75961 aacaggtgaa tgtgctggtg tctgccgttg atagtggtgt gctgaatatt actgactacg
    76021 tcacgccaga tccgtggcag gcgttctttg gtcagaaacg ctatggcgca gacatttacg
    76081 atatttacgg tcaggttatt gaaggtcagg ggcgtctggc agctctgcgt ttcggtggcg
    76141 atggtgatga gctgaaacgt ggtggtaaac cgccggtcaa tcacgtcaat attgtcgcgc
    76201 agcaggcgct gccggtaacg ctcaacgaac agggcgaagg ctcggttaca ctgccgattg
    76261 gcgattttaa cggtgaattg cgcgtcatgg cgcaagcctg gacggcagat gatttcggta
    76321 gcaacgaaag caaagtgata gttgccgcac cggtgattgc tgaactgaac atgccgcgct
    76381 ttatggcgag tggcgatacc tcgcgtctga cgctggatat cactaatctt accgataaac
    76441 cgcaaaaact gaacgttgcc ctgaccgcca gtggtttgct tgaactggtc agcgattcac
    76501 ccgcagccgt tgaattagcg ccaggtgtgc gtactacgct gtttatcccg gtgcgagcat
    76561 tgccgggtta tggcgatgga gaaattcagg ccaccattag cgggttagcg ttaccgggtg
    76621 aaaccgttgc cgatcagcat aagcagtgga aaatcggcgt ccgtccggcg ttcccggcac
    76681 aaacggttaa ttacggtacg gcgttacagc ctggtgagac atgggcgatt ccggcggatg
    76741 gattgcaaaa cttctcgcct gttacgctgg aagggcaatt gttgttgagc ggcaaaccac
    76801 cgctgaacat tgcacgttat atcaaagagt taaaagcgta tccgtacggc tgtcttgagc
    76861 aaaccgccag cggcctgttc ccgtcacttt ataccaacgc agcccaactg caggcgttgg
    76921 gcatcaaagg cgacagtgat gagaaacgcc gtgcatcggt cgatatcggc atttcccgtt
    76981 tgctgcaaat gcaacgtgat aacggcggct ttgcgctgtg ggataaaaac ggtgacgaag
    77041 agtactggct gacggcttac gtgatggatt tcctggtccg cgcaggcgaa cagggttaca
    77101 gcgtgccgac agacgccatt aaccggggta atgagcgtct gctgcgctat ttacaagatc
    77161 cgggcatgat gtcgatcccg tacgcggata atctcaaagc cagtaaattc gccgttcagt
    77221 cttacgctgc gctggtgctg gcccgtcagc aaaaagcacc actgggtgcg ctgcgtgaaa
    77281 tctgggagca tcgtgcagat gccgcttctg gtttaccgct gctgcaactt ggcgttgcgc
    77341 tgaaaaccat gggtgatgca acgcgtggtg aagaagcgat tgtgctggcg ctgaaaacgc
    77401 cgagaaatag tgatgagcgg atatggctgg gtgattacgg tagtccactg cgcgacaacg
    77461 cgttaatgct ctccttgctg gaagaaaata aactgctacc cgatgagcag aacactttgc
    77521 tgaacacgct ttcgcagcag gcatttggtg aacgctggct atcgacgcag gaaagtaacg
    77581 cgttgttcct ggctgcccgt acgattcagg atttaccggg taaatggcag gcgcaaacct
    77641 ctttctcagc tgagccgctg acaggcgaga aaacgctaaa cagcaatctg aatagcgatc
    77701 aacttgccac cttgcaggtg agaaacagtg gcgatcagcc gttatggctg cgtgtggatg
    77761 ccagcggtta tccgcaatcc gcacctttac cggcgaacga tgtgctgcaa atcgagcgtc
    77821 atattcttgg tactgatggt aagagcaaat cgctggactc gttacgtagc ggcgatctgg
    77881 tgctggtgtg gttgcaggta aaagccagta acagcgtgcc ggatgcgttg gtcgtggatc
    77941 tgctgcctgc gggtctggaa ctggaaaacc agaatctggc gaacggtagc gccagcctgg
    78001 agcaaagtgg tggcgaagtg cagaacttac tgaaccagat gcagcaggcg agtattaagc
    78061 acattgagtt ccgtgacgat cgctttgtgg cggcggttgc cgttgatgaa taccaaccgg
    78121 taacgctggt gtatctggcg cgggcggtga cgccgggaac gtatcaggta ccgcaaccga
    78181 tggtggaatc aatgtatgtt ccccaatggc gggcgaccgg cgcggctgaa gatctgctga
    78241 ttgtccgacc gtaaatgcct cgcttgttaa ccaaacgcgg ctgctggata acgctggcag
    78301 ctgcgccatt tctccttttt cttgcagcgt ggggagcaga taaactctgg cctctaccgc
    78361 tgcatgaagt caatcccgca cgagtggtcg tggcgcagga tggtacgccg ctatggcgct
    78421 tcgccgatgc tgacggcatc tggcgttatc cggtaacaat cgaagatgtt tctccacgtt
    78481 accttgaagc gctgatcaat tatgaagatc gctggttctg gaagcatccg ggggtgaatc
    78541 cattctcggt ggcgcgcgca gcatggcaag atctcacttc gggacgggtt atttccggtg
    78601 gcagcacgct cactatgcag gttgctcgtc tgcttgatcc tcaccccaaa acatttggcg
    78661 gcaaaattcg ccagctctgg cgcgcgttgc aactggaatg gcatctgtct aagcgtgaaa
    78721 ttctgacctt gtatcttaac cgcgctccgt ttggcggtac gttgcagggg atcggtgcgg
    78781 caagttgggc ttatctcgga aagtcgcctg cgaatttaag ctattccgag gcggcaatgc
    78841 tggcggtttt gccgcaagcg cccagccgtc ttcgcccgga tcgttggccg gagcgtgccg
    78901 aagccgcgcg taataaagtg ctcgaacgga tggccgtgca aggtgcgtgg tcccgtgagc
    78961 aggtaaaaga gtcaagggaa gaacccatct ggctggcacc ccgacaaatg ccgcaactgg
    79021 caccgctgtt ttcgcgcatg atgctcggta aaagcaaaag cgacaaaatc gttactacgc
    79081 tggatgccgg tcttcaacga cgtctggaag aactggcgca aaactggaaa gggcggttgc
    79141 caccgcgcag ctcactggcg atgatcgtgg ttgatcatac cgatatgcgt gttcgcggct
    79201 gggtgggatc ggttgatctc aacgatgatt cacgctttgg tcatgttgat atggtcaatg
    79261 cgatccgatc gccaggatcg gtgctcaaac cgtttgttta tggtctggcg ctggatgaag
    79321 gcttgatcca cccggcatca ctgctgcaag acgtcccccg gcgcaccggt gattatcgac
    79381 caggtaactt tgatagcggt tttcatggcc cgatcagcat gagcgaggcg ctggtgcgct
    79441 cgctgaactt acctgctgtg caggtgctgg aagcctatgg accgaaacgg tttgcggcaa
    79501 agttacgcaa tgttggattg ccgttatatt tgcccaacgg tgctgcgccg aatctttcac
    79561 tcattctcgg cggcgctggt gcaaaactgg aagatatggc ggcagcgtat accgcgtttg
    79621 ctcgccacgg caaggcaggc aaattgcgct tacagcctga cgatccgctg cttgaacggc
    79681 ctttaatgtc gtcgggggcg gcgtggatca ttcgccggat tatggctgat gaagcgaaac
    79741 ccttgccgga tagtgccttg ccgcgcgtcg ccccactggc atggaaaacg ggcaccagct
    79801 atggctatcg tgacgcctgg gcgattgggg ttaacgctcg ctatgtcatt gggatctgga
    79861 ctggcagacc ggacggcacg cccgttgttg gtcagtttgg ctttgccagt gccgtaccat
    79921 tgttgaatca ggtcaataat atcttactgt cgcgcagtgc gaatctgccg gaagacccgc
    79981 gcccgaactc agtcactcgt ggcgttatct gctggccggg cggacagtct ttgccggaag
    80041 gtgacggtaa ctgtcgccgc cgcctggcaa cctggctgct ggacgggagt cagccgccaa
    80101 ctctattact gccggagcag gaaggcatta atggcattcg tttccccatc tggctggatg
    80161 aaaatggcaa acgtgttgcc gctgattgcc cgcaagcgcg acaagaaatg ataaatgtct
    80221 ggccgctacc gctggaaccc tggctgcccg catcagaacg tcgcgctgtg cgcttaccac
    80281 cggcctcgac aatttgcccg tcatacggtc acgatgccca gctccctctg cagctgacag
    80341 gcgttcgcga tggcgcgatt attaaacgtt taccgggcgc agcggaagcg actttgccgt
    80401 tgcaatcaag tggaggggca ggtgaacgct ggtggtttct gaatggcgaa ccgttaactg
    80461 aacgcgggcg caacgtgacc ctgcatttga cggataaagg tgattatcaa ttgctggtga
    80521 tggatgatgt ggggcaaatc gcgacagtga aatttgtcat gcaatagtct gttttgtccc
    80581 tgattgttgc taaaactcat tttattttaa aaaaatgtta ccttcatcaa tagtcaacgg
    80641 ccctgttgct cattataatc cgcgccatct cgtacgctgg tacagacaac aacagaacaa
    80701 tttacagagg taaaaatggc tattgaacgt actttttcca tcatcaaacc gaacgcggta
    80761 gcaaaaaacg tcattggtaa tatctttgcg cgctttgaag ctgcagggtt caaaattgtc
    80821 ggcaccaaaa tgctgcacct gaccgttgaa caggcacgtg gcttttatgc tgaacacgat
    80881 ggaaaaccgt tctttgatgg tctggttgaa ttcatgacct ctggcccgat cgtggtttcc
    80941 gtgctggaag gtgaaaacgc cgttcagcgt caccgcgatc tgctgggcgc gaccaatccg
    81001 gcaaacgcac tggctggtac tctgcgcgct gattacgctg acagcctgac cgaaaacggt
    81061 acccacggtt ctgattccgt cgaatctgcc gctcgcgaaa tcgcttattt ctttggcgaa
    81121 ggcgaagtgt gcccgcgcac ccgttaataa tttcgtaaat gccgcgtgca aacgtggcat
    81181 ccgtgcgcca gaatttgtac aatgcagcgc ccccggacga gcagccgctc accggggcgt
    81241 ttcttttttc aatcctccag gggccataac gtgtaataac gaggccggaa tagcatatgt
    81301 ctgaacaatt agtcacacct gaaaacgtca ccacgaaaga tggaaaaatc aacctgctgg
    81361 atctcaaccg tcagcagatg cgggagtttt ttaaagattt aggtgaaaaa cccttccgcg
    81421 ccgatcaggt gatgaagtgg atgtatcact attgctgcga caactttgat gagatgaccg
    81481 acatcaacaa agtgttgcgc gacaaactga aagaggtggc ggaaatccgc gcaccggaag
    81541 tggttgaaga acagcgttca tccgacggca ccattaaatg ggcgatcgcc gttggcgatc
    81601 agcgcgtcga aacggtgtat atcccggaag acgaccgtgc cacgctctgc gtctcttcgc
    81661 aggtggggtg tgcgctggag tgtaaattct gttccaccgc ccagcagggc tttaaccgca
    81721 acctgcgggt atcggaaatt attggccagg tgtggcgtgc ggcgaaaatc gtcggcgcag
    81781 caaaagtcac cggtcagcgc ccaatcacta acgtagtgat gatgggcatg ggcgagccgc
    81841 tgctcaacct gaacaacgtc gttccggcga tggaaatcat gcttgatgat ttcggttttg
    81901 gcctgtctaa acgtcgcgtc acgctttcta cttccggtgt agttccggcg ctggataaac
    81961 tgggcgatat gatcgacgtt gcactggcaa tttccctgca cgcgccgaac gacgaaattc
    82021 gtgacgaaat tgtgccgatc aacaaaaagt acaatatcga aacgttcctt gctgcggtgc
    82081 gccgttatct ggagaaatcc aacgccaatc agggccgtgt cactattgag tatgtgatgc
    82141 ttgaccacgt taacgacggc actgaacacg cgcaccaact ggcggaactg ctgaaagata
    82201 cgccgtgtaa gatcaacctg atcccgtgga accccttccc gggcgcgccg tatggacgca
    82261 gctcgaacag ccgtatcgac cgtttctcaa aagtgctgat gagctacggt ttcaccacta
    82321 ttgtgcgtaa aacccgtggt gatgatatcg atgctgcctg tggtcagttg gcgggcgatg
    82381 ttatcgaccg tacgaaacgt accctgcgta aacggatgca gggtgaagcc atcgacatta
    82441 aagcggtctg atagccatta cgccacggtt actttatgta ccgtggcgta atcttttatg
    82501 aatgaagtat taagttgcaa ttgtctggtc aatattaacg gtgcgttttt gctgacttta
    82561 aggcagtatg taacgatgca acagtaagtt agccttagtg aatgtgggct ttgtcacgag
    82621 cacacagacg gtcttatact gtatgataac ggttaactta acggatgttt cgcggtgtgg
    82681 gtgagcatga tggttcaccg gcatctcaat tctcatttaa acgtacctgc agcgaatgaa
    82741 tactgaagcc acgcacgacc aaaatgaagc acttactacc ggcgctcgcc tgcataatgc
    82801 tcgcgaacaa ctaggactta gtcagcaggc cgttgccgag cgactttgcc tgaaggtttc
    82861 cacggtacgc gacattgaag aagataaggc acccgccgat cttgcttcaa cattcctgcg
    82921 cggatatatc cgctcttatg cgcgtctggt acatattcca gaagaagaac tgctgccagg
    82981 gctggaaaag caggctccac tacgggctgc aaaagttgcg cctatgcaga gtttttccct
    83041 cggtaaacgc cgcaaaaaac gtgacggctg gctgatgacc ttcacctggc tggtgttgtt
    83101 tgtggttatc ggcctgagcg gtgcctggtg gtggcaagac cacaaagctc agcaggaaga
    83161 gatcaccact atggccgatc aatcttcggc ggaactgagc agtaatagcg agcaggggca
    83221 gagtgttccg ttaaatacgt cgacaactac agacccggct acaacgtcca cgccgccagc
    83281 gtctgtggat actaccgcaa ccaacacgca aacacctgcc gtaactgcgc cagcaccagc
    83341 tgttgatccg caacagaatg cggttgtttc gccttcgcag gcaaatgttg ataccgccgc
    83401 gaccccggca ccaacggcaa caacaacgcc agatggcgct gcgccgttgc caaccgatca
    83461 ggcaggcgtg actacgccgg cggctgatcc gaatgcgctg gtgatgaact ttactgccga
    83521 ttgctggctg gaggttactg atgctaccgg taaaaaattg tttagcggta tgcagcgtaa
    83581 agacggtaat ttgaacttaa ccggccaggc accatacaaa ctgaaaattg gtgcgccagc
    83641 cgcagtacag atccagtatc aagggaaacc tgtcgatctg agtcgtttta tcagaactaa
    83701 ccaggttgcg cgtctgaccc tcaatgccga acaatcaccg gcgcagtaac agacgggtaa
    83761 cgcgggagat ttttcatgca taaccaggct ccaattcaac gtagaaaatc aacacgtatt
    83821 tacgttggga atgtgccgat tggcgatggt gctcccatcg ccgtacagtc catgaccaat
    83881 acgcgtacga cagacgtcga agcaacggtc aatcaaatca aggcgctgga acgcgttggc
    83941 gctgatatcg tccgtgtctc cgtaccgacg atggacgcgg cagaagcgtt caaactcatc
    84001 aaacagcagg ttaacgtgcc gctggtggct gacatccact tcgactatcg cattgcactg
    84061 aaagtagcgg aatacggcgt cgattgtctg cgtattaacc ctggcaatat cggtaatgaa
    84121 gagcgtattc gcatggtggt tgactgtgcg cgcgataaaa acattccgat ccgtattggc
    84181 gttaacgccg gatcgctgga aaaagatctg caagaaaagt atggcgaacc gacgccgcag
    84241 gcgttgctgg aatccgccat gcgccatgtt gatcatctcg atcgcctgaa cttcgatcag
    84301 ttcaaagtca gcgtgaaagc gtctgacgtc ttcctcgctg ttgagtctta tcgtttgctg
    84361 gcaaaacaga tcgatcagcc gctgcatctg gggatcaccg aagccggtgg tgcacgcagc
    84421 ggggcagtaa aatccgccat tggtttaggt ctgctgctgt ctgaaggcat cggcgacacg
    84481 ctgcgcgtat cactggcggc cgatccggtc gaagagatca aagtcggttt cgatattttg
    84541 aaatcgctgc gtatccgttc gcgcgggatc aacttcatcg cctgcccgac ctgttcgcgt
    84601 caggaatttg atgttatcgg tacggttaac gcgctggagc aacgcctgga agatatcatc
    84661 actccgatgg acgtttcgat tatcggctgc gtggtgaatg gcccaggtga ggcgctggtt
    84721 tctacactcg gcgtcaccgg cggcaacaag aaaagcggcc tctatgaaga tggcgtgcgc
    84781 aaagaccgtc tggacaacaa cgatatgatc gaccagctgg aagcacgcat tcgtgcgaaa
    84841 gccagtcagc tggacgaagc gcgtcgaatt gacgttcagc aggttgaaaa ataataacgt
    84901 gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atagctcccg aaacattgag
    84961 ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca aaaaacattc
    85021 aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg cagcgcattg
    85081 aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc ttgccgattg
    85141 tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg gttgaaaaag
    85201 agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct gaagggacgg
    85261 cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa cagcgtctgt
    85321 ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat cgtcagttcc
    85381 atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct gaactgatta
    85441 tgctcaccgc ccgctggtgg cgcgcgctgg gtatttccga acacgtaact cttgagctga
    85501 attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg gcattccttg
    85561 agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact aacccgctgc
    85621 gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct ccggcattag
    85681 gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa ctgcttgaga
    85741 gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat tactataacc
    85801 gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg tgtgcaggcg
    85861 gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct gtcggttttg
    85921 cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa tttaaagccg
    85981 atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct gcggctatgg
    86041 cattagctga gcgtctgcgt gatgaattac cgggcgtgaa attgatgacc aaccacggcg
    86101 gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc gttgctgtgg
    86161 tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg cgctctggtg
    86221 agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta ctgggttaag
    86281 gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag aagcggttaa
    86341 acgctttttt gctgaaaatg gcaaagcact ggctgttggg gtgattttgg gcgttggcgc
    86401 actgattggc tggcgctact ggaacagcca tcaggttgat tctgcacgct ccgcttctct
    86461 tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca tcccggcggc
    86521 ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt tggaacttgc
    86581 gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac aacaggggct
    86641 ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg ctcgcgttca
    86701 ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca aaggtgaagg
    86761 gtgggctgcc attgttgccg acctgcgtgg tgaagcattg ctgagcaaag gtgataagca
    86821 aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg cactgagcga
    86881 aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg caattgcgta
    86941 aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg ctgtttaaca
    87001 gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag tttacgccga
    87061 ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc aatcttcatc
    87121 cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta aaagcgctga
    87181 atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat ggctggttct
    87241 ctaaagagcc tgcattactt tctggcggtc tgaccgtgtc tggtgggcat gtctacattg
    87301 gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg gcatggcaaa
    87361 ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg gtgttaatcc
    87421 acaccagtaa cggtcagtta caagcgctga acgaggctga cggcgctgtc aaatggacag
    87481 ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca acggcttttg
    87541 gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg gaacagggcc
    87601 agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt gaccgtctga
    87661 gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg gcctataatg
    87721 gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc gaactgggtt
    87781 cggtgaatga tttcatcgtc gacggcaatc gcatctatct ggtcgatcaa aatgaccggg
    87841 tgatggcgtt gaccattgat ggcggcgtta cgctgtggac acaaagcgat ctgttgcatc
    87901 gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtaggtgac agtgaaggtt
    87961 atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa gttgatagtt
    88021 ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag gcaaaagacg
    88081 gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa acggctcctg
    88141 gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt tgtctgatga
    88201 tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg cgccctaacg
    88261 taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg gttgcggatt
    88321 tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc cgtgagttta
    88381 tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc cgcatggcgg
    88441 aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg gatgcgcgcg
    88501 cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt gaaaaaccga
    88561 ccttcctggt ggcaaacaaa actgacggtc tggatcccga tcaggcagtg gttgatttct
    88621 actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt ggcgtattaa
    88681 gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa gaggaagtcg
    88741 acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa gaagaagagg
    88801 aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt cgtccgaacg
    88861 taggtaagtc tacactcact aaccgtattc ttggtgaaga gcgcgttgtt gtttatgaca
    88921 tgcctggcac gacgcgtgac agtatctaca ttccaatgga acgcgatgga cgtgagtatg
    88981 tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct gtagagaaat
    89041 tctccgtaat caaaacgttg caggccattg aagatgccaa cgtggtgatg ttagtgattg
    89101 atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt ctcaatagtg
    89161 ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa gtgaaagagc
    89221 aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt gtgcacttta
    89281 tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt gaagcgtatg
    89341 acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg atggctgttg
    89401 aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat gcccacgctg
    89461 gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac ctgcctgatt
    89521 cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg ggatcgccga
    89581 ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac accctgacgc
    89641 caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat aaataattat
    89701 ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggatggcta ccggaggaat
    89761 tcatatggaa ctgcattgtc cacaatgtca gcatgtgctt gatcaggata acgaccatgc
    89821 gcgttgccca tcctgcgggg aaattattga aatgaaagct ctttgtccag actgccatca
    89881 accgttgcag gtgttaaaag cctgcggtgc agtagattac ttctgtcagc acgggcatgg
    89941 cttgatatcg aaaaaacgcg ttgaattcgt gctggcttaa tgcacctttt tacgcgattt
    90001 ttttaccggc tggatgtttt ttacttcact ttctatccag ccgtcttcca gacgagtggt
    90061 tagcatttca cccgctttaa cttgcttcac ttttttcagt acattgccgt cagtagcagt
    90121 agtaacgctg tatccacgcg ccagcgttga cagtgggctt acggcttcga ggtgcgttac
    90181 tgcattaccg aaacgttcac gcgtggcgct aagctgtacg cgcagggttt ctgctaaacg
    90241 atattccagt tgctgaatgc gcgtttgcgc gcgatgaatc ttcggctgtg gattttgctg
    90301 attcagccgc tgtgttaacc gctgctgctg ttgcccggta cgcttaagtt gattttccag
    90361 cgcaaagctc atccgctttt gcaggcgctc aagcatggtt tgctggcgtg ccagccggag
    90421 ctgcggatgc tgttgctgta atcggtgatg gatctgcgta aagagacgcg tgcggttggc
    90481 gagataataa tccatcgcca tctccagccg ttgacgggtc gattgcacct ggcgcagtaa
    90541 ctcttgctga ttacggctca ctacttcagc ggcggcagac ggcgttggcg cacgcagatc
    90601 ggcaacaaaa tcggcaatgg tcacatccgt ctcatgcccg acggcgctga cgaccggaat
    90661 gcggctggca aaaatcgccc gcgctacgcg ttcgtcgtta aaactccata aatcttccag
    90721 cgaaccgccg ccgcgcccaa cgatcaacac gtcgcactca ttgcgctgat tcgccagttc
    90781 aatggcgcga acagtttgcc ccggcgcgtc atcgccctga acggcggtgg ggtagatgat
    90841 caccggtaga gaaggatcgc gacgttttaa cacatgcaaa atatcatgta gcgcagcacc
    90901 ggtttttgag gtgatcacac caacgcaatg cgcaggggag ggaagtggtt ttttgtattg
    90961 cagatcgaac aatccttcag cctgcaactt cgctttgagc tgttcgtact tctgttgcag
    91021 cagcccttca ccggccggct gcatactctc aacgattatc tggtagtcgc cgcgcggctc
    91081 gtagagcgta atattggcgc gaactaaaac ttgttgccca tgctgtggac ggaaggtcac
    91141 ccgacggttg ctgttgcgga acatcgcgca gcgtacctgg gcggtgtcgt ctttgagtgt
    91201 aaagtaccag tgaccggaag ccggttgtgt gaaattagag atttcgccgc tgatccaaac
    91261 ctgtcccatc tcatgctcaa gcagcagacg aaccgtttga ttcaggcgac taacggtaaa
    91321 aattgcaggg gattgagaag gtaacatgtg agcgagatca aattctaaat cagcaggtta
    91381 ttcagtcgat agtaacccgc ccttcgggga tagcaagcat tttttgcaaa aaggggtaga
    91441 tgcaatcggt tacgctctgt ataatgccgc ggcaatattt attaaccact ctggtcgaga
    91501 tattgcccat gctacgtatc gctaaagaag ctctgacgtt tgacgacgtt ctcctcgttc
    91561 ctgctcattc taccgttctg ccgaatactg ctgacctcag cacccagctg acgaaaacta
    91621 ttcgtctgaa tatccctatg ctttccgcag caatggatac cgtaacggaa gcgcgcctgg
    91681 ctattgctct ggctcaggaa ggcggtatcg gctttatcca caaaaacatg tccattgaac
    91741 gccaggcaga agaagttcgt cgtgtgaaaa aacacgaatc tggtgtggtg actgatccgc
    91801 agactgttct gccaaccacg acgctgcgcg aagtgaaaga actgaccgag cgtaacggtt
    91861 ttgcgggcta tccggtcgtt accgaagaaa acgaactggt gggtattatc accggtcgtg
    91921 acgtgcgttt tgttaccgac ctgaaccagc cggttagcgt ttacatgacg ccgaaagagc
    91981 gtctggtcac cgtgcgtgaa ggtgaagccc gtgaagtggt gctggcaaaa atgcacgaaa
    92041 aacgcgttga aaaagcgctg gtggttgatg acgaattcca cctgatcggc atgatcaccg
    92101 tgaaagactt ccagaaagcg gaacgtaaac cgaacgcctg taaagacgag caaggccgtc
    92161 tgcgtgttgg tgctgcagtt ggcgcaggtg cgggtaacga agagcgtgtt gacgcgctgg
    92221 ttgccgctgg cgttgacgtt ctgctgatcg actcctccca cggtcactca gaaggcgttt
    92281 tgcagcgtat ccgtgaaacc cgtgctaaat atccggatct gcaaatcatc ggcggcaacg
    92341 tggcaacagc tgcaggtgca cgcgctttgg cagaagctgg ttgcagcgcg gttaaagtcg
    92401 gtatcggccc tggttctatc tgtacgactc gtatcgttac tggcgtaggt gttccgcaga
    92461 tcactgccgt tgctgacgca gttgaagccc tggaaggcac cggtattccg gttatcgcag
    92521 atggtggtat tcgcttctcc ggcgacatcg ccaaagctat cgcagctggc gcaagcgcgg
    92581 tgatggtagg ttccatgctg gcgggtactg aagaatctcc gggtgaaatc gaactctacc
    92641 agggccgttc ttacaaatct taccgtggta tgggttccct gggcgcgatg tccaaaggtt
    92701 cctctgaccg ttatttccag agcgataacg ctgccgacaa actggtgccg gaaggtatcg
    92761 aaggtcgcgt agcttataaa ggtcgcctga aagagatcat tcaccagcag atgggcggcc
    92821 tgcgctcctg tatgggtctg accggctgtg gtactatcga cgaactgcgt actaaagcgg
    92881 agtttgtacg tatcagcggt gcgggcattc aggaaagcca cgttcacgac gtgaccatta
    92941 ctaaagagtc cccgaactac cgtctgggct cctgattctc ttcgcccgac ttcatgtcgg
    93001 gcgatttata ttatctgttt cacttgcctc ggaataagcg tcaatgacgg aaaacattca
    93061 taagcatcgc atcctcattc tggacttcgg ttctcagtac actcaactgg ttgcgcgccg
    93121 cgtgcgtgag ctgggtgttt actgcgaact gtgggcgtgg gatgtgacag aagcacaaat
    93181 tcgtgacttc aatccaagcg gcattattct ttccggcggc ccggaaagca ccaccgaaga
    93241 aaacagcccg cgtgcgccgc agtatgtctt tgaagcaggc gtaccggtat tcggcgtttg
    93301 ctatggcatg cagaccatgg caatgcagtt gggcggtcac gttgaagcct ctaacgaacg
    93361 tgaatttggc tacgcgcagg ttgaagtcgt aaacgacagc gcactggttc gcggtatcga
    93421 agatgcgctg accgcagacg gtaaaccgct gctcgatgtc tggatgagcc acggcgataa
    93481 agttaccgct atcccgtccg acttcgtcac cgtagccagc accgaaagct gcccgtttgc
    93541 cattatggct aacgaagaaa aacgcttcta tggcgtacag ttccacccgg aagtgactca
    93601 cacccgccag ggtatgcgca tgctggagcg ttttgtgcgt gatatctgcc agtgtgaagc
    93661 cctgtggacg ccagcgaaaa ttatcgacga tgctgtagct cgcatccgcg agcaggtagg
    93721 cgacgataaa gtcatcctcg gcctctctgg tggtgtggat tcctccgtaa ccgcaatgct
    93781 gctgcaccgc gctatcggta aaaacctgac ttgcgtattc gtcgacaacg gcctgctgcg
    93841 tctcaacgaa gcagagcagg ttctggatat gtttggcgat cactttggtc ttaacattgt
    93901 tcacgtaccg gcagaagatc gcttcctgtc agcgctggct ggcgaaaacg atccggaagc
    93961 aaaacgtaaa atcatcgggc gcgttttcgt tgaagtattc gatgaagaag cgctgaaact
    94021 ggaagacgtg aagtggctgg cgcagggcac catctaccct gacgttatcg aatctgcggc
    94081 ttctgcaacc ggtaaagcac acgtcatcaa atctcaccac aacgtgggcg gcctgccgaa
    94141 agagatgaag atgggcctgg ttgaaccgct gaaagagctg ttcaaagacg aagtgcgtaa
    94201 gattggtctg gagctgggcc tgccgtacga catgctgtac cgtcacccgt tcccgggacc
    94261 aggccttggc gttcgtgtgc tgggtgaagt gaagaaagag tactgtgacc tgctgcgccg
    94321 tgctgacgcc atcttcattg aagaactgcg taaagcggac ctgtacgaca aagtcagcca
    94381 ggcgttcact gtgttcctgc cggtacgttc cgttggcgta atgggcgatg gtcgtaagta
    94441 tgactgggtt gtctctctgc gtgctgtcga aaccatcgac tttatgaccg cacactgggc
    94501 gcatctgccg tacgatttcc tcggtcgcgt ttccaaccgc attatcaatg aagtgaacgg
    94561 tatttcccgc gtggtgtatg acatcagcgg caagccgcca gctaccattg agtgggaatg
    94621 attttaatcc ctgcactatg aatgaacaaa accctctgtt actacagagg gttttttatc
    94681 ttcaagaatc ataggcttga agttactaac atcgattaat taaaccagct gtccgatttg
    94741 ttctcttctg ctttgcccac gcgtttcatc agatcgcgac cgccttcagt catatttctg
    94801 ttggcgtcag cttcagattg caccacatcg gtttgcgcag ctttgtgctt cagttcctga
    94861 tcgataaatt cgttttctcg cttaacgcgg gcttcttctt ttgccagcgc cagtttttgt
    94921 ttctgaatct ctaagctgcg tagctcatct tcataacttt gatcgcgttt tttgtccgca
    94981 gaggcttcgg cgtccagttt atcctgacga gctttcttat ttgccgctgc cgttgccgct
    95041 cttttgttag cggcggcctg ggcatttgcg cgacgttgct tctcttgctg gatttccctg
    95101 ttgcgctccg cgacccattc gtcatgctgc ctttgctctt catttttacc ttgctgttca
    95161 gcttctgcta cagcagagag ttgatcctgc aatgatgagg cgatagccgg atagcttaag
    95221 gaggccaaga tggcgcaaag aaaaactttc ttcatgactc ctcctgatta ttagctcttt
    95281 tcaggacatt tagtatttgg ctgaatacgc gtttcgttat acgttgtggt aataacaacg
    95341 gctaaacctg tcgtaaactg gcactcttta cccacctggg tagaggtata cactttggtg
    95401 ccttccttat atgttaaaga aacaccttcc actaaggttt tatcattcac catagaaccc
    95461 actgccgcgc ctacagctcc gccgccaact gcccctgccg tcgttccgga attgctgcca
    95521 gaaccgacgt tgtggccgat aacaccgcca gcgactgcgc caataagcgc gccgaaggct
    95581 tgtgcgttcc gtttattttg ggagttgtct acggcaactt ttgcgggaag aatggaaata
    95641 atattaacgg ttttagtttc ttgtttggta ttcagttgat cggtttgata aacatcggcg
    95701 gcatgatcgt cagcatttga ctggcatcct gccagagtga atgacgctaa cattgccaca
    95761 ggcagaagac attttttaaa tttcatcact attccttgtt atacacatta cgaaaaataa
    95821 gacatt
//
LOCUS       DAZUFB010000005        91531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZUFB010000005 DAZUFB010000000
VERSION     DAZUFB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.005"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            517..1605
                     /gene="yehL"
                     /locus_tag="ACHY3M_000391"
     CDS             517..1605
                     /gene="yehL"
                     /locus_tag="ACHY3M_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS4315374.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1616..3895
                     /gene="yehM"
                     /locus_tag="ACHY3M_000392"
     CDS             1616..3895
                     /gene="yehM"
                     /locus_tag="ACHY3M_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFS4315375.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARYKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            3888..5024
                     /gene="yehP"
                     /locus_tag="ACHY3M_000393"
     CDS             3888..5024
                     /gene="yehP"
                     /locus_tag="ACHY3M_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS4315376.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5021..7019
                     /gene="yehQ"
                     /locus_tag="ACHY3M_000394"
                     /pseudo
     CDS             5021..7019
                     /gene="yehQ"
                     /locus_tag="ACHY3M_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            7144..7605
                     /gene="yehR"
                     /locus_tag="ACHY3M_000395"
     CDS             7144..7605
                     /gene="yehR"
                     /locus_tag="ACHY3M_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFS4315377.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGTTTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7645..8115)
                     /gene="yehS"
                     /locus_tag="ACHY3M_000396"
     CDS             complement(7645..8115)
                     /gene="yehS"
                     /locus_tag="ACHY3M_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4315378.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(8162..8881)
                     /gene="btsR"
                     /locus_tag="ACHY3M_000397"
                     /gene_synonym="yehT"
     CDS             complement(8162..8881)
                     /gene="btsR"
                     /locus_tag="ACHY3M_000397"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFS4315379.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8878..10563)
                     /gene="btsS"
                     /locus_tag="ACHY3M_000398"
     CDS             complement(8878..10563)
                     /gene="btsS"
                     /locus_tag="ACHY3M_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFS4315380.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10785..11516
                     /gene="mlrA"
                     /locus_tag="ACHY3M_000399"
     CDS             10785..11516
                     /gene="mlrA"
                     /locus_tag="ACHY3M_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFS4315381.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            11576..11683
                     /gene="yohO"
                     /locus_tag="ACHY3M_000400"
     CDS             11576..11683
                     /gene="yohO"
                     /locus_tag="ACHY3M_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFS4315382.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(11664..12395)
                     /gene="yehW"
                     /locus_tag="ACHY3M_000401"
     CDS             complement(11664..12395)
                     /gene="yehW"
                     /locus_tag="ACHY3M_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFS4315383.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(12400..13326)
                     /gene="yehX"
                     /locus_tag="ACHY3M_000402"
     CDS             complement(12400..13326)
                     /gene="yehX"
                     /locus_tag="ACHY3M_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFS4315384.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTSL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(13319..14476)
                     /gene="yehY"
                     /locus_tag="ACHY3M_000403"
     CDS             complement(13319..14476)
                     /gene="yehY"
                     /locus_tag="ACHY3M_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFS4315385.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(14483..15400)
                     /gene="osmF"
                     /locus_tag="ACHY3M_000404"
     CDS             complement(14483..15400)
                     /gene="osmF"
                     /locus_tag="ACHY3M_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFS4315386.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(15611..17908)
                     /gene="bglX"
                     /locus_tag="ACHY3M_000405"
     CDS             complement(15611..17908)
                     /gene="bglX"
                     /locus_tag="ACHY3M_000405"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFS4315387.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            18104..19819
                     /gene="dld"
                     /locus_tag="ACHY3M_000406"
     CDS             18104..19819
                     /gene="dld"
                     /locus_tag="ACHY3M_000406"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS4315388.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGRAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(19857..20789)
                     /gene="pbpG"
                     /locus_tag="ACHY3M_000407"
     CDS             complement(19857..20789)
                     /gene="pbpG"
                     /locus_tag="ACHY3M_000407"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFS4315389.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(20963..21550)
                     /gene="yohC"
                     /locus_tag="ACHY3M_000408"
     CDS             complement(20963..21550)
                     /gene="yohC"
                     /locus_tag="ACHY3M_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFS4315390.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            21720..22298
                     /gene="yohD"
                     /locus_tag="ACHY3M_000409"
     CDS             21720..22298
                     /gene="yohD"
                     /locus_tag="ACHY3M_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS4315391.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(22428..23189)
                     /gene="yohF"
                     /locus_tag="ACHY3M_000410"
     CDS             complement(22428..23189)
                     /gene="yohF"
                     /locus_tag="ACHY3M_000410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4315392.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(23242..24678)
                     /gene="mdtQ"
                     /locus_tag="ACHY3M_000411"
     CDS             complement(23242..24678)
                     /gene="mdtQ"
                     /locus_tag="ACHY3M_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFS4315393.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCGPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            24902..24985
                     /gene="yohP"
                     /locus_tag="ACHY3M_000412"
     CDS             24902..24985
                     /gene="yohP"
                     /locus_tag="ACHY3M_000412"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFS4315394.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(25373..26323)
                     /gene="dusC"
                     /locus_tag="ACHY3M_000413"
     CDS             complement(25373..26323)
                     /gene="dusC"
                     /locus_tag="ACHY3M_000413"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFS4315395.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            26562..26960
                     /gene="yohJ"
                     /locus_tag="ACHY3M_000414"
     CDS             26562..26960
                     /gene="yohJ"
                     /locus_tag="ACHY3M_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4315396.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            26957..27652
                     /gene="yohK"
                     /locus_tag="ACHY3M_000415"
     CDS             26957..27652
                     /gene="yohK"
                     /locus_tag="ACHY3M_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFS4315397.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNVMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            27782..28666
                     /gene="cdd"
                     /locus_tag="ACHY3M_000416"
     CDS             27782..28666
                     /gene="cdd"
                     /locus_tag="ACHY3M_000416"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4315398.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            28816..29535
                     /gene="sanA"
                     /locus_tag="ACHY3M_000417"
     CDS             28816..29535
                     /gene="sanA"
                     /locus_tag="ACHY3M_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFS4315399.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            29538..29777
                     /gene="yeiS"
                     /locus_tag="ACHY3M_000418"
     CDS             29538..29777
                     /gene="yeiS"
                     /locus_tag="ACHY3M_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFS4315400.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            30096..31334
                     /gene="preT"
                     /locus_tag="ACHY3M_000419"
     CDS             30096..31334
                     /gene="preT"
                     /locus_tag="ACHY3M_000419"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFS4315401.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            31328..32563
                     /gene="preA"
                     /locus_tag="ACHY3M_000420"
     CDS             31328..32563
                     /gene="preA"
                     /locus_tag="ACHY3M_000420"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFS4315402.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(32634..33644)
                     /gene="mglC"
                     /locus_tag="ACHY3M_000421"
     CDS             complement(32634..33644)
                     /gene="mglC"
                     /locus_tag="ACHY3M_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFS4315403.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33660..35180)
                     /gene="mglA"
                     /locus_tag="ACHY3M_000422"
     CDS             complement(33660..35180)
                     /gene="mglA"
                     /locus_tag="ACHY3M_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFS4315404.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(35241..36239)
                     /gene="mglB"
                     /locus_tag="ACHY3M_000423"
     CDS             complement(35241..36239)
                     /gene="mglB"
                     /locus_tag="ACHY3M_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFS4315405.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(36519..37559)
                     /gene="galS"
                     /locus_tag="ACHY3M_000424"
     CDS             complement(36519..37559)
                     /gene="galS"
                     /locus_tag="ACHY3M_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFS4315406.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIAPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(37701..38858)
                     /gene="yeiB"
                     /locus_tag="ACHY3M_000425"
     CDS             complement(37701..38858)
                     /gene="yeiB"
                     /locus_tag="ACHY3M_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFS4315407.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(38875..39543)
                     /gene="folE"
                     /locus_tag="ACHY3M_000426"
     CDS             complement(38875..39543)
                     /gene="folE"
                     /locus_tag="ACHY3M_000426"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFS4315408.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            39801..40637
                     /gene="yeiG"
                     /locus_tag="ACHY3M_000427"
     CDS             39801..40637
                     /gene="yeiG"
                     /locus_tag="ACHY3M_000427"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS4315409.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(40669..42660)
                     /gene="cirA"
                     /locus_tag="ACHY3M_000428"
     CDS             complement(40669..42660)
                     /gene="cirA"
                     /locus_tag="ACHY3M_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFS4315410.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(42953..44422)
                     /gene="lysP"
                     /locus_tag="ACHY3M_000429"
     CDS             complement(42953..44422)
                     /gene="lysP"
                     /locus_tag="ACHY3M_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFS4315411.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(44627..45508)
                     /gene="yieE"
                     /locus_tag="ACHY3M_000430"
     CDS             complement(44627..45508)
                     /gene="yieE"
                     /locus_tag="ACHY3M_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFS4315412.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            45607..46656
                     /gene="yeiH"
                     /locus_tag="ACHY3M_000431"
     CDS             45607..46656
                     /gene="yeiH"
                     /locus_tag="ACHY3M_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFS4315413.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            46730..47587
                     /gene="nfo"
                     /locus_tag="ACHY3M_000432"
     CDS             46730..47587
                     /gene="nfo"
                     /locus_tag="ACHY3M_000432"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4315414.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            47591..48665
                     /locus_tag="ACHY3M_000433"
                     /pseudo
     CDS             47591..48665
                     /locus_tag="ACHY3M_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(48721..49971)
                     /gene="nupX"
                     /locus_tag="ACHY3M_000434"
                     /pseudo
     CDS             complement(48721..49971)
                     /gene="nupX"
                     /locus_tag="ACHY3M_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(50071..51012)
                     /gene="rihB"
                     /locus_tag="ACHY3M_000435"
     CDS             complement(50071..51012)
                     /gene="rihB"
                     /locus_tag="ACHY3M_000435"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFS4315415.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            51142..51840
                     /gene="yeiL"
                     /locus_tag="ACHY3M_000436"
     CDS             51142..51840
                     /gene="yeiL"
                     /locus_tag="ACHY3M_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFS4315416.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(51908..53158)
                     /locus_tag="ACHY3M_000437"
     CDS             complement(51908..53158)
                     /locus_tag="ACHY3M_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS4315417.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(53252..54190)
                     /gene="psuG"
                     /locus_tag="ACHY3M_000438"
     CDS             complement(53252..54190)
                     /gene="psuG"
                     /locus_tag="ACHY3M_000438"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFS4315418.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(54178..55119)
                     /gene="psuK"
                     /locus_tag="ACHY3M_000439"
     CDS             complement(54178..55119)
                     /gene="psuK"
                     /locus_tag="ACHY3M_000439"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFS4315419.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(55543..57234)
                     /gene="fruA"
                     /locus_tag="ACHY3M_000440"
     CDS             complement(55543..57234)
                     /gene="fruA"
                     /locus_tag="ACHY3M_000440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFS4315420.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(57251..58189)
                     /gene="fruK"
                     /locus_tag="ACHY3M_000441"
     CDS             complement(57251..58189)
                     /gene="fruK"
                     /locus_tag="ACHY3M_000441"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4315421.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(58189..59319)
                     /gene="fruB"
                     /locus_tag="ACHY3M_000442"
     CDS             complement(58189..59319)
                     /gene="fruB"
                     /locus_tag="ACHY3M_000442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFS4315422.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            59687..60868
                     /gene="setB"
                     /locus_tag="ACHY3M_000443"
     CDS             59687..60868
                     /gene="setB"
                     /locus_tag="ACHY3M_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFS4315423.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTN
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(60865..61119)
                     /gene="yeiW"
                     /locus_tag="ACHY3M_000444"
     CDS             complement(60865..61119)
                     /gene="yeiW"
                     /locus_tag="ACHY3M_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS4315424.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            61274..61846
                     /gene="yeiP"
                     /locus_tag="ACHY3M_000445"
     CDS             61274..61846
                     /gene="yeiP"
                     /locus_tag="ACHY3M_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFS4315425.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            62069..63535
                     /gene="yeiQ"
                     /locus_tag="ACHY3M_000446"
     CDS             62069..63535
                     /gene="yeiQ"
                     /locus_tag="ACHY3M_000446"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4315426.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     DVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            63653..64639
                     /gene="yeiR"
                     /locus_tag="ACHY3M_000447"
     CDS             63653..64639
                     /gene="yeiR"
                     /locus_tag="ACHY3M_000447"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFS4315427.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            64678..65391
                     /gene="lpxT"
                     /locus_tag="ACHY3M_000448"
     CDS             64678..65391
                     /gene="lpxT"
                     /locus_tag="ACHY3M_000448"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFS4315428.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            65803..66369
                     /gene="mepS"
                     /locus_tag="ACHY3M_000449"
     CDS             65803..66369
                     /gene="mepS"
                     /locus_tag="ACHY3M_000449"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFS4315429.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            66550..68106
                     /gene="pdeN"
                     /locus_tag="ACHY3M_000450"
     CDS             66550..68106
                     /gene="pdeN"
                     /locus_tag="ACHY3M_000450"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS4315430.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            68188..70002
                     /gene="yejA"
                     /locus_tag="ACHY3M_000451"
     CDS             68188..70002
                     /gene="yejA"
                     /locus_tag="ACHY3M_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4315431.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAASLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            70003..71097
                     /gene="yejB"
                     /locus_tag="ACHY3M_000452"
     CDS             70003..71097
                     /gene="yejB"
                     /locus_tag="ACHY3M_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFS4315432.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            71097..72122
                     /gene="yejE"
                     /locus_tag="ACHY3M_000453"
     CDS             71097..72122
                     /gene="yejE"
                     /locus_tag="ACHY3M_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315433.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            72124..73713
                     /gene="yejF"
                     /locus_tag="ACHY3M_000454"
     CDS             72124..73713
                     /gene="yejF"
                     /locus_tag="ACHY3M_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFS4315434.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKSSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(73717..74061)
                     /gene="yejG"
                     /locus_tag="ACHY3M_000455"
     CDS             complement(73717..74061)
                     /gene="yejG"
                     /locus_tag="ACHY3M_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFS4315435.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(74394..75584)
                     /gene="bcr"
                     /locus_tag="ACHY3M_000456"
     CDS             complement(74394..75584)
                     /gene="bcr"
                     /locus_tag="ACHY3M_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFS4315436.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(75612..76307)
                     /gene="rsuA"
                     /locus_tag="ACHY3M_000457"
     CDS             complement(75612..76307)
                     /gene="rsuA"
                     /locus_tag="ACHY3M_000457"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFS4315437.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            76456..78216
                     /gene="radD"
                     /locus_tag="ACHY3M_000458"
     CDS             76456..78216
                     /gene="radD"
                     /locus_tag="ACHY3M_000458"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4315438.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            78341..78625
                     /gene="rplY"
                     /locus_tag="ACHY3M_000459"
     CDS             78341..78625
                     /gene="rplY"
                     /locus_tag="ACHY3M_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFS4315439.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(78764..79771)
                     /gene="yejK"
                     /locus_tag="ACHY3M_000460"
     CDS             complement(78764..79771)
                     /gene="yejK"
                     /locus_tag="ACHY3M_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFS4315440.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGMPPNLRDQLQRRTSGG
                     N"
     gene            79953..80180
                     /gene="yejL"
                     /locus_tag="ACHY3M_000461"
     CDS             79953..80180
                     /gene="yejL"
                     /locus_tag="ACHY3M_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFS4315441.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            80200..81960
                     /gene="yejM"
                     /locus_tag="ACHY3M_000462"
     CDS             80200..81960
                     /gene="yejM"
                     /locus_tag="ACHY3M_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFS4315442.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            82035..82111
                     /locus_tag="ACHY3M_000463"
     tRNA            82035..82111
                     /locus_tag="ACHY3M_000463"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82069..82071,aa:Pro,seq:ggg)
     gene            82330..83571
                     /locus_tag="ACHY3M_000464"
     CDS             82330..83571
                     /locus_tag="ACHY3M_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS4315443.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLIKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALIRAGTDPQDHERQKRETEARR
                     QGNTFENVATAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGMIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRVVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            84068..84274
                     /locus_tag="ACHY3M_000465"
     CDS             84068..84274
                     /locus_tag="ACHY3M_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4315444.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTTA"
     gene            complement(84396..84599)
                     /locus_tag="ACHY3M_000466"
     CDS             complement(84396..84599)
                     /locus_tag="ACHY3M_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315445.1"
                     /translation="MTNKESTNTPSQKTNREHENMALEHESERFAPCAFVLDEFLKKY
                     SRSERMKMAAQYGPNKQDNCPPA"
     gene            complement(84657..84830)
                     /locus_tag="ACHY3M_000467"
     CDS             complement(84657..84830)
                     /locus_tag="ACHY3M_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315446.1"
                     /translation="MSTPLEKRSPPYQMRLPEEFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
     gene            complement(85335..85640)
                     /locus_tag="ACHY3M_000468"
     CDS             complement(85335..85640)
                     /locus_tag="ACHY3M_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4315447.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKAKESE"
     gene            85837..86037
                     /locus_tag="ACHY3M_000469"
     CDS             85837..86037
                     /locus_tag="ACHY3M_000469"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4315448.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            86253..86698
                     /locus_tag="ACHY3M_000470"
                     /pseudo
     CDS             86253..86698
                     /locus_tag="ACHY3M_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            86691..86924
                     /locus_tag="ACHY3M_000471"
     CDS             86691..86924
                     /locus_tag="ACHY3M_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315449.1"
                     /translation="MRDIYLETIDRASLALSHSENMMEILRMCLESFGDNERNAKKTR
                     IITSLITLLESVINELQEIETLHDRYNEQHTGE"
     gene            86930..87229
                     /locus_tag="ACHY3M_000472"
     CDS             86930..87229
                     /locus_tag="ACHY3M_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4315450.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            87226..88626
                     /locus_tag="ACHY3M_000473"
     CDS             87226..88626
                     /locus_tag="ACHY3M_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFS4315451.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSQKRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKL
                     ENDGVIVIDGDDVRPL"
     gene            88828..89073
                     /locus_tag="ACHY3M_000474"
     CDS             88828..89073
                     /locus_tag="ACHY3M_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315452.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            89204..89398
                     /locus_tag="ACHY3M_000475"
     CDS             89204..89398
                     /locus_tag="ACHY3M_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315453.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            89402..89563
                     /locus_tag="ACHY3M_000476"
     CDS             89402..89563
                     /locus_tag="ACHY3M_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFS4315454.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            89691..90179
                     /locus_tag="ACHY3M_000477"
     CDS             89691..90179
                     /locus_tag="ACHY3M_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4315455.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDQKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            90191..90352
                     /locus_tag="ACHY3M_000478"
     CDS             90191..90352
                     /locus_tag="ACHY3M_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315456.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDDDF"
     gene            90342..91265
                     /locus_tag="ACHY3M_000479"
     CDS             90342..91265
                     /locus_tag="ACHY3M_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4315457.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPCTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT        22747 a        23804 c        22885 g        22095 t
ORIGIN      
        1 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
       61 taacttttgt gtcagcgagt ccatccgctc tccttattaa ggagcgcaac accagcacct
      121 tacccttaca ctgcctgtcg atactggttt acgtatgttg gaccacgatg ttgcactccg
      181 gatcagggca ccacctgcag caccccccgt ccggtagcca ggacttgaaa taggttcgat
      241 ccggcgcaac gtcccaacag tcaggattat aaatctcatt atcttaattg caacggggtc
      301 cagccgtggt tataattcgt tcgttggaac acgatgttgc acaggctgtg gtgttaggcc
      361 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtaatctg
      421 ctttccagat attttccgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
      481 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
      541 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
      601 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
      661 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
      721 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
      781 aaatctctcc tgtctgaatt actggcaacc gctattagcg gcgatgccgg gttaaccatc
      841 cagggcggcg catctaccac tgaagatcaa atcaagtacg gctggaacta cgctctgctt
      901 atcaatcatg gcccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
      961 gacggcaaaa tcgtccgttt cgaagagata acgcgtacac cgctggaagt gcaagattgt
     1021 ctgttaggta tgctttctga cagagttatg acggtgccgg aactcactgg tgaagccagt
     1081 caactctatg cgcgggaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     1141 gtcaatgaga tgagtgccgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     1201 atggatttcg ctcaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     1261 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     1321 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     1381 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     1441 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     1501 gaagaagatc gcgctcgtct gcgccgttac ttcgaacaac gagtcgccac acataaagaa
     1561 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     1621 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     1681 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggcccggctg attttaatga
     1741 ccgggtagac gaactgtttt tatcccacca gcttccggta gctatttaca gttattgcca
     1801 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     1861 ggagtggcag gcgctacaag ccgcacgtcg tattcaggca caaacttact tcatcgattt
     1921 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag atgaaagcca
     1981 ggccttactg ctgcgtgcca cccgcatgga taacagcgat accctgtggg atcacttgtt
     2041 cgaagatgaa agccagcaaa ctgcattacc ctctgcgctg gcgcactatt ttgcccaact
     2101 gcggggcgat ttccccggcg atgcactcaa tcgtcagcgc gaagccttta tggctcgctg
     2161 gattgcatgg gcggtgcagc aaaataatgg cgacgtgtta gtcgtctgcg gtggctggca
     2221 cgctccggca ctggcaaaaa tgtggcgcga atgcccgcag gacattaaca cgccggaatt
     2281 gccctcgctg gcagatgcca ttacaggttg ttatctcacg ccctacagtg aaaagcgcct
     2341 tgatgtgctg gcaggatacc tttccggtat gcctgccccg gtctggcaaa actggtgctg
     2401 gcagtggggc ttacagcagg ccggtgaaca actgctaaaa actatcctta cccgtttgcg
     2461 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     2521 gctggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     2581 aggctcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     2641 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     2701 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cctgcgaact
     2761 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
     2821 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
     2881 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
     2941 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcaa cactccagga
     3001 agccgcacgc tataaattag aagccgatat gctggacgcg ggtggaatcg gcagtatcac
     3061 cacatgtctt agccaggcgg cgttagcggg tctggcgtcc ttcagtcaac aattactgga
     3121 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
     3181 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
     3241 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctatgtgaat ctaacggcag
     3301 accagatgaa aaggagtttc acgctcacct gcatagctgg caagcgttgt gtcatatcct
     3361 gcgcgatcta catagcggtg tgcaactccc cggcatttct ctttctgcgg cggtagcctt
     3421 actggagcga cgcagtcagg caattcatgc cccggcgctg gatcgcggcg cggctcatgg
     3481 cgcactgatg cgtctggaac atcctaacgc cagtgccgaa gcggcgctga cgatgctggc
     3541 gcagttatcc ccggcacaat ccggcgaagc gctgcacggt ttgctggcgc tggcccgcca
     3601 tcaactggcc tgtcagccga catttatcgc cggtttcagc agtcatttaa atcaactaag
     3661 tgatgccgat tttatcaatg ccctgcccga tttacgcgcg gcgatggcct ggctaccacc
     3721 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
     3781 cgtttcggcg ctgcaaatgc cgttgcattg tccaccacag gccattgcac atcatcaaca
     3841 actcgaacag caggcactgg catcgctgca aaactgggga gttttccatg tctgaactga
     3901 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
     3961 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agaccacgcg ctggagtggc
     4021 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctggtgga ttaggtggct
     4081 caaatctcac cacccctgag tggatcaaca gtattcacac tctgtttccg caacaggtga
     4141 ttgagcgact ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacgaatc
     4201 tcgacgtgct ggaacgtatg cagccttcgg aaagcctgct acgcgccgtt ttgcacacca
     4261 aacatctgat gaatcccgaa gtactggctg ccgcccgcca gatagtgcgc caggttgttg
     4321 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
     4381 gccgtcgccg ctcattaatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
     4441 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta
     4501 acagccgcat taaacgccaa agcgaacaat ggcaactggt cttactggtt gatcaaagcg
     4561 gatcgatggt ggactcggtg atccactctg cggtgatggc ggcctgtttg tggcagttac
     4621 ccggcattcg tacccatctg gtggcgtttg acacaagcgt ggttgatctc acggcagacg
     4681 ttgccgatcc ggtagagtta ttaatgaaag tacagttggg cggcgggacc aatatcgcca
     4741 gtgccgtgga gtatggtcgg caacttattg aacaaccagc aaaaagcgtc attatcctcg
     4801 tgagcgattt ttacgaaggg ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg
     4861 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcaccgca acgccttgct
     4921 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacgc
     4981 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
     5041 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
     5101 cagtcttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acggcgcttt
     5161 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacagccagg cactgaaaga
     5221 ggctcagtgc agctgcgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
     5281 ttatcaacga ctttgtgcca ccgtccagcc cacggaaaaa gaagaagagt gggatccggc
     5341 aatctggctg gaagaactgg ctaccctgcc cgatgccacc cgcaaacgcg cgcaggcgct
     5401 ggtcgctaaa ggcatcacca ttgagttgtt ctgtgcgccg ggcgaaattc cctctgcccg
     5461 cttaccgatg agcgatgtgc gtttttattc ccgtagcagt attcgtttcg cccgttgtga
     5521 ttgtattgaa ggcacacttt gcgaacatgt cgtactggct gtgcaggcct tcgtcgaggc
     5581 caaagcgcag caagcagaat ttaaccattt aatctggcag atgcgcagcg aacacgtcac
     5641 atcatctgac gatccgtttg ccagcgaaga aggccaaacg tgtcgtcaat atgttcagca
     5701 attaagccag gcattatggc ttggcggcat cagccagccg cttattcact acgaggcagc
     5761 attcaaccgc gcattgcagg cggcagaggc ctgcaactgg cgctgggtga gtgaatcact
     5821 acgacagcta cgggcaagcg ttgatgcctt ccacactcgc gccagccact atcatgccgg
     5881 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
     5941 ccggcgcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
     6001 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
     6061 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
     6121 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg
     6181 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
     6241 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
     6301 gccgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
     6361 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
     6421 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
     6481 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctcc tgacgttatc
     6541 ccttccggca tctgcctgtt ctccttttgc cgttgaacgc atggcggcgc ttttgcaaca
     6601 aacagacgac cccgtgagtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
     6661 actggaacca cgggtgatga tgacaaaaac ccgcgcctgg gcgctggacg cggaaactgc
     6721 gcctgtggca cctaccttct gccagcgttt tgcctgtgcc gtctaccgct catcagttgc
     6781 tgatgcgctg ccaggcgtta cttattcaac tgctccataa cggctggcgc tatcaggaac
     6841 agagcgctat tagtcaggca gagttgctgg cgaatgacct ctccgcggtc ggtttttatc
     6901 ggctggcaca tgtgttggga caatttcgta atacagaaag cgaggcacgg gtagaagcaa
     6961 tgaataacgg tgttttactt tgcgaacaat tattccccat gcttcagcaa caaggatgaa
     7021 atagtgcttt ttactaagag ttctactcca gttccggact gctcacgtca cggtattagg
     7081 catattctat atagcccctg gtgagagtca ccagtttctt gattaaataa aatggagttt
     7141 tacatgaagg ctttcaataa gctgttttcc ctcgttgttg catctgttct ggttttctct
     7201 cttgctggct gcggtgacaa agaagaatcg aagaaattca gcgccaatct gaacggcact
     7261 gaaattgcca ttacctatgt ctacaaaggt gacaaggtgc ttaagcaatc ttctgaaacc
     7321 aaaattcaat ttgcttctat tggtacaacc accaaagaag acgctgccaa gacacttgag
     7381 ccgttaagcg ccaaatacaa aaacatcgcg ggtgttgaag aaaaattaac ctatacagat
     7441 acctacgcgc aggaaaacgt gactatcgat atggaaaaag tggattttaa agccctgcag
     7501 ggtatttcag gaatcaacgt ttctgctgaa gatgccaaaa aaggtatcac tatggcgcaa
     7561 atggaactgg tgatgaaagc cgctggtttt aaagaagtga aataatcggt tggcggtcat
     7621 gctctaaaca tgaccgccaa ttttttagcc ttttttcaca tgctggcgcg ctgccagtcc
     7681 acgcagaaaa taacgtaaaa attgatcgcc gcattcgcgg aagtttttat gatccggtgc
     7741 gcgcatcatc gctgtaattt ccggcatcga aacgcggaac tgctgttcgg tgaggatagc
     7801 cagaatgtca tcggttttca gcgaaaacgc gatgcgtaat tttttcagca cgatgttgtt
     7861 attaatgcga cgttccggct ccagtgccgg agcagactca tccttgccgc gtttttcata
     7921 aatcaggcca ttgaggaatg acgacaaaaa aatgtccgga caacgctgaa aaccctcttc
     7981 gtcttcttta cgtagccaga cggcgatctg ttccgcggtg gcttcgacat tacccagcgc
     8041 cagaatacgc accaggtcat tattattggc tttcaaaatg tagcgcacgc tgcgcagaat
     8101 atcgttactt agcatgaggc cttcaggtgt tgatgaggca aaaagccatt ttagcagtct
     8161 tttacaggcc aatcgcctct tttaagcttt tcagataacg gcggctgacc ggcacggtta
     8221 agccattacg caaaatcaac tcggcctggc cgttatcttc cagacgaatc tcctgtaaat
     8281 gcgcgaggtt aaccagatac tgacgatggc agcgcagtag tggtgtacga ctttccaggg
     8341 tacgtaatgt caattcggta aagccctctt tcccttcgtg gctggtaacg tagacaccgc
     8401 tcatccgact gctgacaaat gccacatctt tcatttgcag caaataaatc cgactatgcc
     8461 ccgtacaagg gataaatttc agcgcctgtt gattttccgg taacagcgaa acatcctgct
     8521 tgctgcgctc ctgacgcaat cgcgccagcg ttttctccag tcgcgcttca tcaattggct
     8581 tcagcagata atcaaaggca tgttcttcaa aggctttaat agcgtattcg tcaaacgcag
     8641 tgagaaaaac aatatacggg cgatgttccg ggtcaagcat ccccaccatt tccagaccac
     8701 tgatgcgcgg catctggata tcgagaaaca gcacatccgg gcgcagttta tgcaccgcgc
     8761 cgatcccttc cacggcgttt gaacactctc caacgatttc aatatcgctc tgctcctgca
     8821 aaaatacgcg caggttctcc cgtgctaacg gttcatcatc gacaattaag actttaatca
     8881 tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg taactatcag gctcacaggc
     8941 gacgcttatt ccatagtcat cgccaaaccg ttcacgtaaa cgcttatcca ccagattcat
     9001 ccccagccca ctggcattgg ttaccggttg atacaaaccg gcattgtctt cgatctccag
     9061 catcaaatgt tgcccctcac gtcgggcgct gattgccact cgccctgtat ccagcagttg
     9121 tgatgtccca tgtttaatgg cgttttccac tatcggttgc agggtaaacg cgggcaattg
     9181 ctgctgggat aattcttgcg gaatagcaat gttgacctgc aaccgcgact ggaagcgcgc
     9241 cttttcaatt tgcagataag cgttcacatg ttcaatttcg tcggcgagag taacaaactc
     9301 cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga tactgcacca gctggctggc
     9361 ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta agcgcattaa acaaaaaatg
     9421 aggattcacc tgggcgtgaa gcagtttgat ttctgactgg gtgagcatcg ctttttgccg
     9481 ctcatattgc ccggcaagaa tctgcgccga aagcagttgc gcaatcccct cgcccagcgt
     9541 gcggtttatt gaactgaata aacggttttt ggcttcatac aatttgatgg tgcccatcac
     9601 gcgctgattt tcgccacgca acggaattac cagcgtcgac cccagtttgc attgcggatg
     9661 caaagagcaa cgataaggta cttcgttgcc atcagcgtag accacttcac cggtttcaat
     9721 cgcttttaag gtgtaagtcg aagaaatcgg tttgccgggt aaatggtggt cgtcaccaat
     9781 tccggtaaag gccagcaatt tctctcgatc ggtaatcgcg actgcaccaa tatccagctc
     9841 ctgatacagc acctgagcca ctttcatgct gttcacttcg ttaaacccct gtcgcaaaat
     9901 gccttccgtc gaggctgcca ctttcagcgc agtggcagaa aaagcagaag tgtatttttc
     9961 aaacatcgcg cgtttatcga gcaatatacg cataaacagc gccgcgccga cggtattggt
    10021 gaccatcatt ggtgcagcaa tattactcac cagacgcacc gcatcttcat aaggtcgggc
    10081 gatcgcaagg atgatcagca tttgcaccat ttcagcgacg aacgtgacgg caccggcggt
    10141 aatggggtta aagactttat cagtgcgccc gcgacggatc aggatgctgt gtaccaggcc
    10201 gccgagcaat ccttcaacga tggttgagat catgcaactt agcgcggtca tgccccccat
    10261 cgaatatcga tgtaagccgc cggtcagacc aaccagccca ccgacgaccg gaccgccgag
    10321 taagccgccc attaccgcgc ctatcgcacg ggtattggca atagaatcgt caatgtgcaa
    10381 cccaaaccag gtgcccatga tgcagaagat ggaaaagacg atgtagcaga gaaatttatg
    10441 cggcagacga accgtgacct gcattaacgg tatgaataat ggcgttttac tcattaacca
    10501 cgcaatgact aaaaaaacgc acatctgctg aagcagcagc aacaccagat taaaatcgta
    10561 catacccgca aaccacactt ccctttaaaa cgcgtaacat acattgcctg cgtttaactt
    10621 tctttgaact cttgcagaaa aatgagaatt cgtgagtacg atcactcaaa atcgcctggc
    10681 aaaaataaaa tcaccctata gatgcacaaa aaacgggcaa aactacctgg ttcgcaaaac
    10741 tgcgtctaaa gttaaaccgg gacctcgcga gcaagggtga gacgatggcg ctttacacaa
    10801 ttggtgaagt ggcgttgctt tgtgatatta accctgtcac gttacgcgcg tggcagaggc
    10861 gttacgggtt gctgaaaccg caacggacag acggcggtca tcgactgttc aacgatgccg
    10921 atattgaccg tatccgcgag atcaaacgct ggatcgacaa cggcgtgcag gtcagcaaag
    10981 ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc gatcagcaag
    11041 aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg tggatcaaag
    11101 agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt cctctgcgcc
    11161 gacgacttca atgccaacaa ccgactctcc aggcgctgct ggcgatcctc gacggcgtac
    11221 tgatcaacta catcgccatt tgtctggctt cggcacgtaa aaaacagggt aaagatgcgc
    11281 tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc tggattgcca
    11341 gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta cgccctgaac
    11401 tattcgaagg tcgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc gcccaacagc
    11461 agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc atttaatgat
    11521 tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt gcattatgcg
    11581 catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttcggcg gaattggtgg
    11641 cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg caccagcctt
    11701 tcaaacaggc ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc ctggatcaca
    11761 tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt gctggcccct
    11821 accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc
    11881 agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc acgactcatt
    11941 cccatacctt tcgcaacttc tgtcacgctg gcatcgatcg ctcccagccc ggcaagtgtc
    12001 gcctgcagga cgggcagcac accgtaaagg atcaaggcga taatcgctgg ttgcagacca
    12061 aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt ctgtccaacg
    12121 gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt gacagcaatt
    12181 ccggcaccag tgccaatgat caccgcaaac aaactcgaaa ttcccaccag ccagaaatga
    12241 gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag ttgtgggaac
    12301 aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac aaacaaagca
    12361 atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcca ccagcagatc
    12421 ctgaaaatgc agcgtaccgc aaggctggcc ctgcgtgttc accaccggca gcacctcgca
    12481 accccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc
    12541 accttctgcc cgttcttcgc gacgcacgta atccgccaca ctacgtaacg aaagcagacg
    12601 cacacccagt tcgctacgtc caaaaaactg gcggacaaaa tcattcgccg gacgagtcag
    12661 catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc
    12721 agccagccgt agcgcctcat caatatcatg agtgaccagc acaatagtgc gccccagcaa
    12781 acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc
    12841 aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg ccactcccac
    12901 acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgcaaat ttgactccag
    12961 ccccagtagc gtcattaatt cgtcgatacg atcgtcaatc cgtgcccgcg accatttttg
    13021 taattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaata ggccaataga
    13081 ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc
    13141 tccggcaaag cggatctctc cgctgtcatg ttccaccagg cggttaatca ttttcaagga
    13201 ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt
    13261 gagattaaga tcgttaacgg ctttttgtgc gccaaacagt ttgctgacat ggctaaattc
    13321 aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagggca tcgatcagca
    13381 ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca
    13441 gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca
    13501 tacctacagt ttgcaccatc accacccgca ggctgcgcag aaataccggt aacgccagtg
    13561 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
    13621 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
    13681 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcttcc
    13741 ccagccacgg aaaggccgtc accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
    13801 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
    13861 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
    13921 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
    13981 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
    14041 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
    14101 catcgctaca ggccagcaat gccagcgccg cagccagcca gaaaccactg ccgaggctgg
    14161 tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc cgcctttccc gcgccccaca
    14221 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct tttttaccgg
    14281 gaataaaaca ggccgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
    14341 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtagctga
    14401 taaacggcag cgctgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
    14461 tacgtagata agtcacggga aattacttcg cccacccttt ttgtttcagg tagtcggcag
    14521 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
    14581 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactccttca
    14641 acaccgattc acgcaccact ggtgcaggcg catagatagg ttgcacgcct tgcggatcgc
    14701 ttaaggtttg cagccccagc gccgctaccg agccgtcagt gccgtaagcc attgcagcat
    14761 taacgccaga ggtttgctgg gcagcggctt tgatcgtcac cgccgtgtcg ccaccagcca
    14821 gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaac gcgggtaacg
    14881 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctcttgca
    14941 gataacgact caggtcggca agcgaagtga gtttattttt ctctgccaca tcctgacgca
    15001 cggcgatagt ccaggtgtta tttgcaggcg cgggcgtcag ccagattaac ttgttgtgct
    15061 ccgaatcgag ctttttgact ttctcgtaac cttgctgcgc gtttttccac gctgcatcgt
    15121 tttcatcttt aaagaagaaa gcgccattgc cggtatattc cggatagata tccagttcac
    15181 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
    15241 gtacgccgtg gctttccagc acctgcaaaa tgatattgcc gagtagcgca ccttcggtat
    15301 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
    15361 acataaccag tgaacctgcc cagagcttta agagtggcat gatgctttcc tcattctttt
    15421 actgttgttt tcagcgaatt aagagaaaag catagttgat aatggcgggg ttagcttgag
    15481 cggaaggatt cagttgcaga atcagataaa tacttaagag gcatattcgg tccggcgttt
    15541 cctgccggat gcggcgcgag cgccttatcc ggcctacaaa gggcgcaaac gtcgcgccct
    15601 tactaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa tcagtgccga
    15661 taaatacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc cagaacttca
    15721 gcgcctcaat atcgatcggg aagctgacag tctgagtttc gcccggtttc agggtgattt
    15781 tctcaaagcc tttcagctgt ttcacagggc gactcatgga agccgtcaca tcctgcaagt
    15841 acatctgcac taccgtggcc ccctcgcgct taccggtgtt cgtcacctgc acgctggcag
    15901 tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag acggtgaaag
    15961 tggtgtagct cagcccatag ccgaacggat acaacgcccc gttagcttca tcaaaataac
    16021 gcgaagtgta tttgttcggc ttgtcggcat tatacgggcg accggtattc agatggctgt
    16081 agtacaccgg gatctgcccg acagaacgcg ggaaggacat tggcagcttg ccggacgggt
    16141 tgtaatcgcc aaacaataca tcggcaattg cattaccgcc ttcagtcccc gcaaaccagg
    16201 tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga cgcccgttca
    16261 tcagcaccag caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag tcacgttggc
    16321 tttgcggaat agtgatatcg gtccggctgg aggcttcgtg cgccatcccc tgtgcttcac
    16381 cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca tcaatcatct
    16441 cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg aaatcgataa
    16501 tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg ttttcaccga
    16561 cggaattttt aatcccggtc agtacggtca cggattgatc ggcaacaccg gctgcggacc
    16621 agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca atggtggccg
    16681 attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt tcgcgcgcca
    16741 cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt ggtatccacc gggtcagact
    16801 ctttcggccc caaatggctg tatgggtcgt taaacaatcc catatcatat ttaacgttca
    16861 gtacatggcg ggcagcatcg tccagctctt ccatcgtcac tttgccggat ttgatcaacc
    16921 caggcagata cttcgagtaa tactcgtcgc tcatactcat gttgattccg gatttcagcg
    16981 ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg tttaatcagc tctttgattg
    17041 caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga acatctttca
    17101 gcagccagga atcggaggtg gctggcgtgc cgttcagcga gttcagcgcc accatcaccg
    17161 cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta aacaggcgct
    17221 gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg tatgcggcaa
    17281 agtgtttgac gctggtcatc accgagtagc gatctgccgg gcttttaccc tgcatcgctt
    17341 ccaccatggt tttacccatt gttgaggtga gatacgtatc ttcgccaaaa ccttcggaag
    17401 cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc atattcaggc
    17461 catcatctgc cgcttcataa gcagagacac gtccgaccgt tttcactgca tcgaggttaa
    17521 aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc agcacgtcgt
    17581 aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc tgcatggcgc
    17641 ggatatcctg acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct ttgatcatct
    17701 cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc tgaccaattt
    17761 tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc gcttcgggcg
    17821 ttaatggatg gttgccgaat aaatcatccg ccagtgctgg ctgcagggcc agactcaccg
    17881 cgattcctac tgaacatagc catttcatat ggatttttct tcctctgttg cccgacgagg
    17941 cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga gaaaaagaat
    18001 gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat acaaggcgct
    18061 attctagttt gtgatatttt ttcgccacca caaggagtgg aaaatgtctt ccatgacaac
    18121 aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt cacacctgct
    18181 caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc agggcgacgc
    18241 gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga aagcctgtgt
    18301 caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg aaggatcgac
    18361 gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctgc gtctcgacaa
    18421 gctgcacgtt cttggcaagg gcgaacaggt gctggcctat ccgggcacca cgctctattc
    18481 gctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg gatcgtcgtg
    18541 tataggcgca tcggttatcg gcggtatttg taacaactcc ggcggctcgc tggtgcaacg
    18601 tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagacg gcaaactgac
    18661 gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc ttagcaagct
    18721 ggatgatgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg cccacgatta
    18781 tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt ataacgccga
    18841 tccggatcgg ttatttgaat cttctggtcg cgccgggaag ctggccgtct ttgcggtacg
    18901 tctagatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca ccaaccagcc
    18961 ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc tgccggttgc
    19021 cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca aagacacctt
    19081 cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc tcaagggacg
    19141 caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg accgtgcgat
    19201 gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa actggcgcga
    19261 taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg aagccaaatc
    19321 gtggttggtg gattatttca aacaggccga aggcgatttc tttgtctgta cgccggagga
    19381 aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc gttatcaggc
    19441 ggtgcattcc gatgaagtcg aagacattct ggcgttggat atcgctctgc ggcgtaacga
    19501 caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc acaagctcta
    19561 ttacggccat tttatgtgct atgtcttcca tcaggattac atagtgaaaa aaggcgtgga
    19621 tgtgcatgcg ttaaaagaac agatgctgga actgctacag cagcgcggcg cgcaataccc
    19681 tgccgagcat aacgtcggtc atttgtataa agcaccggag acgttgcaga agttctatcg
    19741 cgagaacgat ccgaccaaca gcatgaatcc ggggatcggt aaaaccagta aacggaaaaa
    19801 ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga attcacttaa
    19861 tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt tttatagctc
    19921 aacgctgctg ccggcacagg catcacttta ccggtttcaa tccaggtacg caggcggctg
    19981 gcatcggcaa aatgggtata tttgccaaac gcgtccatca ctaccagcgc caccggttta
    20041 ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa gccggttttg
    20101 gttaactgaa tattccagtt atcgcgatac accagatgat tagtattgcg gaacggcagc
    20161 gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa ctgcccgatc
    20221 aacggatact gtttgctggc aatgagcagt ttggttaagt cacgggcagt tgaaacgtta
    20281 tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag cgatttcgct
    20341 ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg cgcaaggctt
    20401 gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt acggctgatt
    20461 tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg gctgatatcc
    20521 acttttagtt tttcatccag cggcagtcgt gcatccagca caaccatcgc ggtcattaat
    20581 ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat cactttgttg
    20641 gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga agcggtggta
    20701 gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat cagggccagg
    20761 ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt tcacgcgcac
    20821 gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca taacgagcaa
    20881 aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca tttacgccag
    20941 caatgcaatg tcaaagaact ttctagaaca ggcgataccc gtagccccat agtataacgg
    21001 ttagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa ctggagaagc
    21061 taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa agcaggtagc
    21121 cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa ccagaccagc ggataaagcg
    21181 ccaccagacc gcttaaaaac agcggagttg caacgtagcc cgcgaagacc atgcagtgcg
    21241 caagtgacgg acgctgcggg taattgcgcg ccatccacca gatgacccgc cccatcaccg
    21301 caaccccagc gagcataacg ccataaaaca ggacagccag cgccagtccg gtaaaccagg
    21361 acaacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg ccaatgaagg
    21421 cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg tgagaaatcg
    21481 tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac agtccccaga
    21541 catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa gtataagtca
    21601 gcttgtgatt attttttgtt cgctcatgta atttgcaccg tctgatgtac gtcctttttc
    21661 agcgtgaatg atttccaggg tgtatgctta caggcaacca aagggagaca gactggccta
    21721 tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggtgatc ggtagcctgg
    21781 cggaaggtga aaccgtgact ttgctgggag gcgttgcggc gcatcagggg ctattaaagt
    21841 tcccgctggt ggtactttct gtggcgcttg gcggcatgat tggcgaccag gtgctttatc
    21901 tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat caggataaaa
    21961 ttgagcgggc gcagaaactt atccaacgcc atccgtatct gtttgtcatt ggtacgcgct
    22021 ttatgtatgg ctttcgggtt attggcccga cgctgattgg tgccagccag ctgccgccga
    22081 aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt tttaccacta
    22141 tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgat cagcatttga
    22201 agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc tggtggctga
    22261 aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct ggataaggcg
    22321 ttcgcgccgc atccgacatc cggtgcacca tgcctgatgc ggcgcttgtg cgtcttatca
    22381 tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta ttctgggttg
    22441 aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc ggtggtgtaa
    22501 tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatacgt tgcgccaaaa
    22561 cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc catgccgttc
    22621 attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg cctgaccaac
    22681 tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt gtaggcgctg
    22741 gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg accaccctgc
    22801 ccttgtttca ccatctggcg ggccgcaatt tgtgagcata aaaatgcacc atcgacatca
    22861 acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcgc tttggtcatt
    22921 gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaatgag tttctccagc
    22981 gcctgtgcac cttctggcag tttaccgaga tccagctgca caatctccgc acgtacgccg
    23041 tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg ccaggtaatg
    23101 ccaatatcaa acccctgctg cgccagtaat aacgcgcact ctttgccgat ccccgaatcg
    23161 gaggcggtaa taatcgcaac ctgtgccatc gagttctcca cttaacgctg aataaacgtt
    23221 aagtatagaa ggcgcatatc atcagcgttt gtaacccccg cccaacgcac cagtgagttg
    23281 aatggaagca tccagccact gcccttgcaa taacaggcca ttggcccgct cacgcagcgc
    23341 ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa agcgcgcctg
    23401 cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct ggtttttctc
    23461 cgcaagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt caaccaccgc
    23521 tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga gattggcgtt
    23581 aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca tttgctgcgc
    23641 ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg ccatcaggtt
    23701 gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg actcaacgta
    23761 ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca gttcatcagg
    23821 aagctggctt gccactttcg gcaacgcgac cggtttaagc ttcaatgact ttgtctggtt
    23881 atttgtaagt gcgcttaacc gtgcctcaat aattttcatt ttccccgcga catcgttgag
    23941 ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg aagaagtaat
    24001 cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt tttctatttg
    24061 ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc gggctacgct
    24121 gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa ccgtacccag
    24181 gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc ccgccgttaa
    24241 gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat cgttcagagc
    24301 aaacggcccc attaagcctt ctgccgacat tttttgccgc tccatatccg cagaaaagtc
    24361 gatctgcgga ccatcctgag tagcaattgc cttcgcctgg gcttcagcta gctgaatgcg
    24421 ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta aggaagtgag
    24481 ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt tcagcgccgt
    24541 gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct ggcgggtttc
    24601 atgcataggc ccacacccgg ccagcatcag taacagcgga aaacaggcga tggcgggata
    24661 aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg agattaccgt
    24721 gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata aaacgacata
    24781 tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc gtttatggat
    24841 aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata aaggaacgcc
    24901 aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac tggtggtgac
    24961 tggcgtattt aagatgatat tttaaaaatc agatatggca tataagttgt caggtcagga
    25021 actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg tcaaaatcgc
    25081 tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgtcttac
    25141 aaggacgtta cctcgcgccc gacccggtag acgaagggga taagctgaag gtaccgcgca
    25201 gcggcgattt tgttcgccag agcccggggg tgcaggggga ggaggcgatt gtcctccccc
    25261 tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga
    25321 tgatgaagct gaataagcga ttcattgtct ctgataaact gcgattccac tattatcgca
    25381 gcttatcgat atcaattgcc tgaatagccc ttgcaatatc aggggaatta ttcaacaccc
    25441 gaacatgctg aaataattcc gttgcttcat cgtattcttt acgcaaataa ctcaaccact
    25501 gtttaatccg cgcaacgtga tataacccgg tatcgccctg cttttccaga cgggtatatt
    25561 tttgcagcaa agcaaccacc tccggccacg gcattcgcgg ttcgttatat tttactaccc
    25621 ggctcaggtt gggaatattg agcgccccgc gaccaatcat cactgcgtcg cagccgctga
    25681 tcgcaatgca ttgttgcgcg ctctgccagt cccagatttc gccgttggca atcaccggaa
    25741 tattcagccg ctggcgaatc tcgccaatcg cctgccagtc aatatgctcc gcgcggtaac
    25801 cctgctcttt tgtccgtcca tgcaccacca gctccgtagc accagcctgt tgaaccgcat
    25861 cggcgatttc aaatttcttc tcaccgctgt cccagcccag acgcactttc acgctgacgg
    25921 gcaaatgcgc cggtacagct tcacgcatcg cttttgcacc ctggtagatg agttcaggat
    25981 ctttgagtaa cgtcgcccca ccaccgctac cgttgaccgt tttcgacggg cagccgcaat
    26041 tgagatccac gccccaggaa cctaattcca ccgcacgggc ggcgttctct gccagccatt
    26101 gtgggaactg acccaacaac tgcacgcgca ccagcgtacc ggatggtgtc cggctggcgt
    26161 tttgtagctc agggcaaatg cgatgaaaaa cttttaccgg cagcagttga tccaccacgc
    26221 ggacaaactc ggtgatgcac agatcgtagt cgttaacttc ggtcagcaat tcacgcacca
    26281 gagagtcaag cacaccctcc atcggagcca gtaacacacg catatcttca cccgcaaaaa
    26341 aatgaggcgc tatgttagcg cctatggtca acggattaaa ggtctgcaaa gagggattgc
    26401 tgcaaactgt aatccctctc tatgcttgat gtatgcctga gttcgttgcg cattgcgtaa
    26461 tgccggggag cgaaaaaata agcgctgatt tcttaatgtg atcggtagca cgttttacga
    26521 attaattgta tgatgaatcc atctcatctg gggtgttgat tatgagtaag acactgaaca
    26581 ttatctggca atatttacgc gctttcgtcc tgatttatgc ctgcctgtat gcaggcattt
    26641 tcattgcttc cctgctaccg gtaaccattc cgggcagcat catcggtatg ctgatcctgt
    26701 ttgtcctgct ggccttgcaa attcttccgg caaaatgggt caatccgggg tgctacgtac
    26761 tgattcgcta tatggcgcta ttgtttgtgc cgattggcgt aggcgtcatg caatattttg
    26821 atttgctccg cgcacagttt ggcccggtag tggtttcctg tgcaatcagt acgctggtgg
    26881 tttttctggt ggtgagctgg agttcgcaac tggtacacgg tgaacgtaaa gtcgtaggtc
    26941 agaaaggatc agaagaatga tggcgaatat ctggtggtca ttaccgctga cgttgattgt
    27001 cttttttgcc gcccgcaaac tggcggcacg gtataagttt cccctgctta acccgttgct
    27061 ggtagcaatg gtggtgatca ttcctttttt aatgctgacc ggcatctctt acgacagcta
    27121 ctttaaaggt agcgaagtgc taaacgatct gctgcaaccg gcggtcgtcg cgctggccta
    27181 tcctttatat gagcagctac accagatccg cgcgcgctgg aaatcgatca tcaccatctg
    27241 ttttatcggc agcgtggttg cgatggtgac ggggacttcc gtggcattgt tgatgggcgc
    27301 ttcaccggaa atcgccgcgt caatcctgcc gaaatcagtc accacgccta ttgcaatggc
    27361 ggttggcggc agtattggcg gtattccggc aatcagcgca gtttgcgtga ttttcgtcgg
    27421 catcctcggc gcggtatttg gccatacatt gcttaatgtg atgcgtattc gtaccaaagc
    27481 tgcgcgcggt ctggcaatgg ggactgcctc gcacgccctc ggtacggcgc gctgcgccga
    27541 gctggattat caggaaggtg catttagttc gctagcgctg gtgttatgcg ggataattac
    27601 ttcgctgatc gcaccgttcc ttttcccgat tattctggca gtaatgggct aaaatttgcg
    27661 atgcgtcgcg catttttgat gtaagtttca cgcgttgcat aattaatgag attcagatca
    27721 catataaagc cataacgggt tcgtaaactg ttatcccatt acatgattat gaggcaacgc
    27781 catgcatcca cgttttcaaa ccgcttttgc ccaacttgcg gataacttgc aatctgcact
    27841 ggaacctatt ctggcagaca agtacttccc cgctttgttg accggggagc aagtctcatc
    27901 gctgaagagc gcaacggggc tggacgaaga cgcgctggca ttcgcactac ttccgctggc
    27961 ggcggcctgt gcgcgtacgc cattgtcgaa ttttaatgtt ggcgcaattg cgcgcggtgt
    28021 gagcggaacc tggtatttcg gtgccaatat ggaatttatt ggtgcgacaa tgcagcaaac
    28081 cgttcatgcc gaacaaagcg cgatcagcca cgcctggttg agtggtgaaa aagcgcttgc
    28141 agccatcacc gttaactaca cgccttgtgg tcactgccgt cagtttatga atgaactgaa
    28201 cagcggtctg gatctgcgta ttcatctgcc gggccgcgag gcacacgcgc tgcgtgacta
    28261 tctgccggat gcctttgggc cgaaagatct ggagattaaa acgctgctga tggacgagca
    28321 ggatcacggc tatgcgctga ctggtgatgc gctttctcag gcggcgattg cagcggcaaa
    28381 ccgttcgcac atgccttaca gtaagtcgcc aagcggtgtc gcgctggaat gtaaagacgg
    28441 tcgtattttc agtggcagct acgctgaaaa cgccgcattc aacccgactc tgccaccgtt
    28501 gcagggagcg ttaattctgt tgaatctcaa gggttatgat tacccggata tccagcgcgc
    28561 ggttctggca gaaaaagccg atgcgccgtt gattcagtgg gatgccacct ccgcaacgct
    28621 gaaagctctc ggctgtcaca gtatcgaccg agtgcttctc gcttaagcct ggtgccggat
    28681 gcggcgtgaa cgccttgtcc ggcttgccag ccctctcctg gtgtcgaaat tcccggcaaa
    28741 cagtttgccg tttcttgcgc aaaaccagcg ggtaaagtag cctgatggaa attttcctta
    28801 gatcgagtct cctgcatgtt aaagcgcgtg ttcctcagcc tgttagtcct gatcggcttg
    28861 ctgctgttga ctgtgctcgg cctcgatcgc tggatgagct ggaaaaccgc gccttatatc
    28921 tacgacgaat tgcaggatct cccctaccgc caggtcggtg tggtgctcgg aacagcaaaa
    28981 tattatcgta ctggcgtaat taatcagtat tatcgctacc gcattcaagg agcgattaat
    29041 gcctataaca gcggtaaggt aaattatcta ttactgagcg gcgataacgc attgcaaagt
    29101 tataatgagc cgatgaccat gcgcaaagat ttaatcgctg ctggtgtcga cccatcagat
    29161 attgttctcg attacgcagg ctttcgtacg ctggattcca tcgtgcgtac acgcaaagtt
    29221 ttcgatacta atgatttcat tattatcacc caacgtttcc actgtgagcg ggcattattt
    29281 attgcgctgc atatggggat tcaggctcag tgttatgccg taccgtcacc gaaagatatg
    29341 ctgtcagtac gtattcgcga atttgccgcc cgtttcggtg cgctggctga cctttatatt
    29401 tttaaacgtg aaccgcgttt tttagggccg ctggtcccta ttccggctat gcaccaggtg
    29461 ccggaagatg cgcaggggta tcccgccgtc acacccgaac agttacttga attacaaaag
    29521 aaacaaggaa agtagttatg gatgtacagc agttttttgt cgttgccgtt tttttcctta
    29581 ttccgatatt ttgtttccgc gaagcatgga aaggctggcg cgcaggcgcg attgataaac
    29641 gggttaaaaa tgcaccggaa ccggtgtatg tctggcgagc aaaaaatccc ggactctttt
    29701 tcgcttatat ggtggcatat atcggcttcg gaattttatc tatcggcatg attgtttatc
    29761 ttattttcta tcgttaatat tttctctttt ctgttaatta ttgagaataa ttattacttc
    29821 actcaataag ctgcggatat aacattctga accgcagcta tttgtgaatc ctttcacagt
    29881 ataaatttcc ccacgcgcat agccttaata tcagcacgca atatttatac tctaaataat
    29941 tcgagttgca ggaaggcgac aagtgagtga gtccccagga gcttacataa gtaagtgact
    30001 ggggtgagcg aacgcagacg cagcacatgc aacttgaagt atgacgagta tatttagcgt
    30061 tcgccttaat tactcatagc attaaggaag atcacatgcc gcaacaaaat tatctggatg
    30121 aactcactcc ggcttttacg cctttactgg cgattaaaga agcctctcgc tgtttattat
    30181 gtcatgacgc tccctgtagt caggcttgcc cggcacagac cgatccgggg aaatttattc
    30241 gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac aattcgcgaa aataatgccc
    30301 tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt atgtcaaagc ggttgtaccc
    30361 gtgccggtgt tgatactccc attgatatcg gtcgcttaca gcgttttgtt actgattttg
    30421 aacaacaaac cgggatggaa atttatcagc ccggtactaa aacgctcggc aaagtcgcga
    30481 ttattggcgc aggtcctgcc ggattgcagg ccagtgtaac actgacaaac cagggttatg
    30541 acgtcacgat ttatgagaaa gaagcgcacc ccggtggctg gttgcgtaac ggtattccgc
    30601 aattccggtt accgcaatca gtgctggatg cagagatcgc ccgtattgaa aaaatgggcg
    30661 tgaccattaa gtgcaacaac gaagtcggta acacactcac ccttgagcag ctgaaagcag
    30721 aaaaccgcgc ggtactggtc accgtggggt tatcgagcgg ttccgggtta tcgctgtttg
    30781 agcatagcga cgttgagatt gccgtcgact tcctgcaacg tgcacgacag gcgcaaggcg
    30841 atatcagcat tccacaaagc gcattaatta tcggcggcgg tgatgttgcg atggacgtag
    30901 ccagcacgct gaaagttctc ggctgtcagg cggtaacctg cgtagcgcgt gaagagttag
    30961 atgagtttcc ggcaagcgaa aaagagtttg ccagcgcccg ggagctgggc gtttcgatca
    31021 tcgatggatt cacgccagta gccgtggaag gcaataaagt cacgtttaag catgtacggt
    31081 taccgggcga actgacgatg gcggcagata aaattattct cgccgtcggt cagcatgcca
    31141 gactggatgc ctttgcggag ttagagccgc agcgtaacac catcaaaaca caaaattatc
    31201 agacccgcga tccgcaagtc tttgctgctg gcgatattgt tgagggtgac aaaaccgtgg
    31261 tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat tcatcactat ttagagggag
    31321 cttgctcatg ttaacgaaag atctttcgat tactttttgc ggcgtgaagt ttcccaaccc
    31381 gttctgcctc tcttcttcgc ctgtaggcaa ctgctatgag atgtgtgcca aagcctacga
    31441 cacaggttgg ggcggcgtgg tgtttaaaac gatcggcttt tttatcgcca acgaagtctc
    31501 gccgcgtttt gatcatctgg tgaaagaaga taccggtttt atcggcttca aaaatatgga
    31561 gcagattgct gaacatccgt tggaagagaa tctggccgcc ctgcgtcagc tgaaggaaga
    31621 ttacccggac aaagtattga tcgcttcgat catgggggaa aatgagcagc aatgggagga
    31681 gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc gagtgtaact tctcctgtcc
    31741 gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa agcccggagc tggtagaaaa
    31801 atattgtcgg gcagtgaaac ggggttccac gctgccaatg ctggcgaaga tgacgccgaa
    31861 tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc ggcggcgcag atggcattgc
    31921 ggcgattaac accgttaaat ccatcaccaa tatcgatctt aatcagaaaa tcggtatgcc
    31981 gatcgttaac ggaaaatcga gtatttccgg atattccggt aaagcggtaa aaccgatcgc
    32041 cctgcgcttc attcagcaga tgcgcaccca tccagaactg cgcgatttcc caatcagcgg
    32101 tatcggcggc attgaaacct gggaggatgc ggctgagttt ttattgctcg gcgcagcaac
    32161 gttacaggtg accaccggca tcatgcagta cgggtatcgg atagtggaag atatggcaag
    32221 cgggttgtcg cattatctcg ccgatcaggg atttgattcg ctgcaggaga tggtaggtct
    32281 ggcgaataac aatattgtcc cggcggaaga tttagaccgc agttatattg tctatccccg
    32341 tatcaatctt gataaatgtg ttggctgtgg acgctgttat atttcctgtt acgacggcgg
    32401 tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg cattgtaata ccgagaaatg
    32461 tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt tgtattgagc tcggggaagt
    32521 gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg taaaaccaga gacgcatccg
    32581 gcatttggca ccgattgccg gatgcagcgc atccggcaat atccacaatc acatcacttc
    32641 ttacgcgcgt atttcagtga atccagcgct acggcgaaga taataatcgc ccctttgatg
    32701 atgtactgcc agtatgggtt tacgccgata tacgtcaggc catagttgat gacggtaaaa
    32761 ataattaccc cggtcaccac gccaatcacc gtccccacac cgccgctgaa cgatacaccg
    32821 cctaccacgc acgccgcgat agcatccagc tcatacataa agccgaggtt gttggtggca
    32881 gagccgatac gtccggcttc taacatcccg ccaaaggcat agaacacgcc agacaacgcg
    32941 tagatcatca gcaggttcag gccgacgttg acaccagata cttttgccgc ttccgggtta
    33001 ccgccaatgg caaaaatgtt cttaccgaag cgggttttgt tccacaacac ccagacgaac
    33061 gccaccgcaa tcaacgcgta gaaggtgatg taagagagac ggaaactccc cagcgcgaca
    33121 aagccctgag caaaggtaga gaagccactg tcaaaaccag aaattggcga cgccccgaca
    33181 aagtcgtaat agagcgagtt gattccatag acgatgatca tcgtgccgag cgtggtaatg
    33241 aacggcgtca cgttgagata agcgataatc agaccgttga tcaaaccgat caccgcacca
    33301 atggcacaga caatcagaat aaccagcgca atcggcatcg tcgccatttc cgggaacact
    33361 ttgttggcgt tatccatgga ctgcaataat gtcgcagcca ccactgccgc cagccctacc
    33421 tgacgaccag cagaaagatc ggtcccctgg gtgacaatta accctgccac accgagcgcg
    33481 ataataatac gcaccgatga ctgggtgaga atattactta agttcaacag acttaaaaat
    33541 gttgggtcct ggaaaataat aatcgccagc aaaactaaaa gaacgacgta aataccgccc
    33601 tctttcaggt aagtaagaaa acttttctta tttaacgcac tcatgggaag cccctaatct
    33661 taaaggtgca aagacgcaag acgcagaatt tcgttttgcg ttgttgtttt tgtatcgaca
    33721 attccggaaa cgagaccatt gctcatgacc agaatacggt ctgttatccc taacaactca
    33781 ggcatttcag aggagataat aataatcccc ttgcctttct tcgccagttc ggcaattaac
    33841 tgataaattt caaacttcgc cccgacatca ataccgcgcg tcggttcatc gagcattaat
    33901 atttctggtt gcgttagtag ccagcgacca ataatcacct tttgctgatt accaccggag
    33961 agcgaaccaa tttgcgtccg atgacccggc gtttttaccc gcatcgaatc aatcacccac
    34021 tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa ctttattttt gtagttgcga
    34081 atattggaaa ttaacgagtt aaaactaata tccagatagg cataaattcc cgttgagcgg
    34141 cgctcctcag ttaccagtgc aaatccgtgg tttatggctt cgttggcatt atggttattg
    34201 atctgtttgc cgtgcaacgt aatggtgcca gccgatttct cgcgaatacc aaataacgtc
    34261 tcaacaatat cggtacgttt cgcccccacc agcccggcaa taccgaggat ctccccttta
    34321 tgcagatcaa acgagacatc gcgaatcgac ggctggcgca gtgacgttag gttacgcacc
    34381 tcgaggatga cttcgcccgg cttgttttct ttgtcaggaa aacgctgatt aagagaacgc
    34441 ccaaccatca tggcgatgat cttgtccatc gtcagtcctg ccagcggttc ggtggcgatc
    34501 cactgaccgt cgcgcaatac ggtaacttca tcacataact ggaagatttc ttccatttta
    34561 tgcgagatat aaacaatacc gcagccgcgc tcttttaatt tacgaataat agtgaacaga
    34621 tgattgacct ctttttcggt taacgaagaa gtcggttcat ccataatcac aattttcgcg
    34681 ttataggaaa acgctttggc gatttcgatc atctgcattt gcgaaacgga taatgtgccg
    34741 acgcgcgcac gcggatcgat atcaatatcc agttcatcaa aaatcgcttt ggtttcgcgg
    34801 tacattttgt cctgatcgac aaacatgcct ttggtgggat atcgccccag ccacatgtta
    34861 tccatcaccg aacgttgtaa taccaggttt aactcctggt gtaccatcga aataccattt
    34921 tccagtgcct ctttggcgga atggaaatcg atctctttac cctggaataa aatggtgccg
    34981 gagtcttttt gataaatacc aaacaggcat tttaataatg tcgattttcc tgcaccgttt
    35041 tcccccatta atgcatggat agaatgtggc cggactttta aattaacgtt atcaagtgcc
    35101 ttaacaccag gaaaggactt gttgataccg ctcatttcca acaagtattc cccggaagac
    35161 ggagtcgttg agctgaccat ataattttac cttgttggcc atacaataag ggcgcagtaa
    35221 acgactgcgc ccaatcagtc ttatttcttg ctgaattcag ccaggttgtc tttatctacg
    35281 ccaacataag gtacgcggac cactttgttg tcgattttcc agttggtgcc atcagccgca
    35341 cctttgccat cggccaggtt tttcgccaga tcaaaggtcg ctttcgcctg gttgttagca
    35401 tcgttcagta ctgtgcccgc cagtgcaccg gatttcacca gcgccagcgc ttctggcagc
    35461 gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg ctttcagtgc ttctaccgcg
    35521 cccattgcca tcgcatcgtt gttggcgata accacttcga ttttgttggc gttcgggcca
    35581 gacagccagg cgtccatctt atctttcgcc tgagcggtat cccacatagc ggtatctaac
    35641 tgtaactgtt cagttttaat gcctttgtcg ttcagctctt taatcacgta agtggtacgt
    35701 gcttctgcat ccggatggcc cggttcacct ttcagcaata cgaattgaat ctgaccgtct
    35761 ttgttcagat cccaaccctg attcgccgcc cagtgtttag caatcaaatc gccctgaata
    35821 atgccggact ctttggaatc agtgccaacg tagtaggctt tgtcgtagct atccagcgcc
    35881 ttacgagacg gttctttgtt gaagaaaacc accggcacgt tttgcccacg cgctttctca
    35941 atcaccgtac ccgcagctgc cgggtcaacc aggttgattg ccagtgcctt cacccctttc
    36001 gccagcaata cgtcgatctg atcgttctgc ttggactggt cattctgaga atcattcatc
    36061 agcagctgaa catctggcgc ggctttcgca tcttgctcaa tagccttgcg cactacagac
    36121 ataaagttat cgtcgtactt atagattgtt acaccaatgc gagtatcagc agcgtgtgca
    36181 gcggcaccga ataacatgct ggccatcaca gcagacaggg ttaacacctt cttattcatg
    36241 gtatctccgg tttttcttat gcagggtagt gcttgagata aatgctcggc ggggcagtag
    36301 agttaatgaa gtgttactga acgccgaagc tcacttttta aaattcgttc ttccatgccc
    36361 ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg ctattacctc ggtgaaagtg
    36421 attattcact gttacatacg ggttacaacg ttaaaacggt gcaatcatag ctatcacatt
    36481 gttaagatac tgtgaaatca ctcacagatt gaaagcggtt acatcgcctg atttgttgag
    36541 ttagtgatcg tcgccgcatt ctggcgtgtt gcgacagaat ggcgacgcac taacgtcggc
    36601 ataaaacagt ggctagcacg aggagcaata ttgcctgctg ccccctgcaa ggccagttcg
    36661 gtggctaatt tcgccattga agcaatggga taacgcacgg tcgttaattg cgggtcggtg
    36721 taacgggcga tgggaatatc atcgaaaccg atgattgaga gatgtaacgg aatcgcaatg
    36781 ccattatctt ttaatgctgt cagtgcgcca gcagccatat tgtcgttata agcaaataca
    36841 gcggtaagtt gtagattgcg ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg
    36901 tccggcgtac cagtgccaat ccagctttcc ggcggaataa tatcctgctc tttcaacgca
    36961 ctcatccagc ctgctttacg catggcgtca tcttcaatgc cgtgactgga agaaagataa
    37021 ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg ccattcgggc accgctgaga
    37081 ttatccaggc aaacgcaacg atgggcgtac cccggcacaa cgcggttgat taacaccata
    37141 ccgggaatgt tatccataaa ttgcgccagt tcatcgtcac tcaatgcttt tgagtgaaca
    37201 atcaacgcat tacaacgctg gcgaattaac acctcaatgg cgtgacgctc tttttccgct
    37261 tcatgatagc tattgccgat tagcacgtat ttctgatgct gctgagcgac cagatccacc
    37321 gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca tcaccaccac gccaatggtg
    37381 tcgctaacct gagttgccag cgcctgggca ttggcgtttg gccgataatc cagctcactc
    37441 acggctttca ttactgcttc acgcgtgtcg gcactgacca gcgtgctgtt attgagcacc
    37501 cgggaaaccg ttgccacaga gacgccagcc tgacgcgcta catcacgaat ggtgatcata
    37561 ttcactacct gttatgagat tgcagtaaat gactgcttgc tggcggctat tttgtcagca
    37621 ctgaatacag gacttcgtga atcgagtcac agcaatggaa acggttacag ccgttttgtt
    37681 aacgaatgtg atccagatcg ttatcttgat gttttagata ttgccgttcc ggcagcacgc
    37741 aaagttaact gacgccagag ccattccacc ggcccctggc ggaagtaacg cagccagata
    37801 acagagaaga ggatattcgc cagccatacc ggaataacaa acgccagcag ctccaggcgg
    37861 tcaaaatgca taaacaaacc gaggtggtaa aaaagcatgg tacaaatcag cgtttgcaat
    37921 agatagttgg tcagcgccat ccgtccgacg caggcgatcg caagcaccag cttaaagcgg
    37981 ctcaattgcg gccagaagcc ataaaacagc gacgcatagc caatcgcctg aaacggcgca
    38041 ctcagttccc gcggcatttg aagtaagaag gcacaccagc gatacgccca gtccagctgc
    38101 cattgcaggg cgatggcagg aaggttaatg gtcaccccaa tcgccaccag cacaaaacca
    38161 gtacgacgat agtgacgtaa gctgaactgc cctttcagcc agccgctgcg catcagcgct
    38221 gcaccaatga gcatcatccc agccagttgc cagccatact gtgcgcccag tgccagtaaa
    38281 ctgttgccaa caccatcggc acgattactg atcgcttcaa cgccgccgtg aagcttccag
    38341 tatttttcat acaaaatagc cgatgcatcc ggcgtccagg cgcggctggt ctggctgtcg
    38401 gaaatcagcc ccaataacag caaaacgcca agccccacca gataaagcat gacgcctgta
    38461 ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc gccagcagat taagcccacc
    38521 agcccgtaag ccagcagaat atcgccgtcc cagaacaata aaccgtgaat aaagcccaat
    38581 aagactaaca gcgttaaccg cgactggatc cagcgtctgc cacggggcag caacatttgc
    38641 agtccagcac caaacagcag cgcaaaaagc gtgaggaatt tcacctgggc gatgagatca
    38701 agaaatgccc aggtccatgc atcctgcggc gtaatagcac cgtaccaggc gggattgaga
    38761 taagccgcct ttggtagccc aaaggcgctg atgtttaata gcaggatccc cagaatggcg
    38821 acgccgcgaa caaaatcgag cgtgacgttg cgctccatgg ttcctgcctt ttaatcagtt
    38881 gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc tgactggatt tgaacaatcc
    38941 accaagagag gtcgttgttg tggcactggt tgcatcgcgg atgccacgcg ccttcacgca
    39001 gtaatgcacc gcgtcgatcg agacagccac gttattggtg cccagcagcg tttgtagcgc
    39061 aataagaatt tgctgcgtca gacgttcctg cacctgcgga cgctgggcaa agaactgcac
    39121 gatacggtta atttttgaca gaccgatcac cgaatctttc gggatataag ccaccgtcgc
    39181 tttgccatcg atggtaacaa aatggtgttc acaggtactg gtcagagtga tatcgcgcac
    39241 ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg gtgattttcg ggaagttggc
    39301 gtaatccaga ccggagaaaa tttcatcgac atacatttta gcgatgcgat gcggcgtttc
    39361 catcaaactg tcatcagcca ggtcgagatt cagcagctgc atgatttcgg tcatatgacc
    39421 agcaataagg cttttgcgcg tttcgttatc catttcatgc acgggcgggc gcagcggtgt
    39481 ttccagtcct cgcgcaacta acgcttcatg aaccagggcc gcttctttac tgagtgatgg
    39541 catttatgat ttctcctgca ggtgtgacgc ctccgccctg cgtgggggca aagttattaa
    39601 gctgatttac agcttgatta ttgtgcgtga ggcggcgcac ataatccagt attcacagcg
    39661 ataattattg taattgccgc tgcctttcat cagcagatgt taaaacatcg ttatgcaaat
    39721 acggaagtga aagttactca cagcacattg aataaacggt atgatgaaga aattgcaaac
    39781 aacacaacaa ggagccacgc atggaaatgc tcgaagagca ccgctgtttt gaaggctggc
    39841 agcaacgctg gcgacacgac tccagtacct taaactgtcc gatgacgttc agtatctttc
    39901 tcccaccacc tcgtgatcac accccgccac cagtgctgta ctggctttcc ggattaacct
    39961 gcaatgacga gaacttcacc atcaaggcgg gtgcccagcg cgtggcggca gaactgggga
    40021 ttgtactggt gatgccagac accagcccgc gcggcgaaca ggttgccaac gatgatggct
    40081 acgatttagg ccagggcgcg gggttttatc ttaacgccac gcaaccgccg tgggcgacgc
    40141 attaccggat gtatgattat ctgcgcgatg aattaccggc gctggttcag tcgcaattta
    40201 atgtcagcga ccgctgcgcc attagcggtc attcaatggg tggtcacggt gcgctgatta
    40261 tggcgctgaa aaatccgggt aaatacacca gcgtttcggc ctttgcgcca attgtgaatc
    40321 cgtgcagcgt cccgtgggga atcaaagcgt ttagcagcta tttaggtgag gacaaaaatg
    40381 catggctgga atgggacagt tgcgcgctga tgtatgccag taacgcgcag gatgcgatcc
    40441 cgacgcttat cgatcagggc gataatgatc agtttcttgc cgaccagttg caacctgcgg
    40501 tactggcaga agccgcgcgc cagaaagcgt ggccgatgac gctgcgtatt cagccgggat
    40561 atgatcacag ttactacttc atcgcctctt ttatagagga tcacctgcgc ttccatgcgc
    40621 agtatttact gaagtgaaag tccgcccggt tcgccgggca tcttctcatc agaagcgata
    40681 atccactgcc ataaagtaac gacgtccgtc ttcgttatag ctgtagtcgt cacgactgag
    40741 atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg acgtctttag tcacctgcca
    40801 ggccgcgccg gtattccaga tggtataacc gcccggtgtt ttagccgtcg cgctgtcggc
    40861 gcgtttctgc ccggtatagt gaccagaaac atagaatgac cagtcttcca gcgccagcgg
    40921 tttccagtcc agcgtaccgt tagcagtatg gaacggcaga tcggatagcg gtttgttttc
    40981 gccgttgctg acatcacgac catcgttgta ggtgtagttg atcgacagtt tccattcatc
    41041 gttgaacgga attttcagtt cggtttccac gccctgaata cgagctttgt taacgttgta
    41101 gtagctaaat accggtatgc gccgtccgtt agcgcccgtc tcaaaaccaa caaagttttg
    41161 gtagcccggt gcagcgttga cgtcagacgt acggctgatg ctgatacgat ctttcacatc
    41221 gttacggaaa acggtaacgc tggattcaac cccttccagc cagccttctt cacccatgta
    41281 gtaaagcccc agctcccaac tttcgctggt ttctggtttc agatctgggc tacccacaat
    41341 cttacatgcg ccacggcagg aattgctcgt ccagtcaggg ctaagttgca acagagaagg
    41401 tgctttaaat gccgtcgccc agcccccttt caccgttacg gtgtcggtgg cgttataaac
    41461 caggtaggca cgcggactcc agtgttcacc gtaggtttcg tgatcgtcca tacgcacgcc
    41521 ggtcgtcagc gccagcggct cgaagatccg ccattcatct tccacaaaca gcgcgtactg
    41581 gctggcagac gttttggagc tggttccccc ggtcaggttc accgcatcgc taagtttgtc
    41641 gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatt gccgtcagcg gcaacgtgta
    41701 tttgccgtcg accgtattgc tttcggaagt tatcgggctg ctgttgccag ggtttttgtt
    41761 ctcgactttc tcaccgtagt atttcagttc gctggtgccg taatcccaac gcccattatg
    41821 gctgacggag tagttctggc gttccaggcg gtttttgtcc agcgagtcgg aatcacgatc
    41881 ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt tgattcggtg tccaggcaaa
    41941 ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc ggcgtttctc cggtatcggt
    42001 ggtcgttgag ttttgcgggt catccttttc acgttttgcc aggctgccgt aagctttcat
    42061 tcccagcaca ccatcaatta atggtccact ggtaaagaac tgaccgttat aggtatcacc
    42121 gcgatcgcga tgttcctgaa tggtggtatc gacggtaacg gtacccgacc atttctgacc
    42181 gatttttttg gtgatgatat tcactacacc gccgagcgca tcggagccgt atagcgacga
    42241 catcgggcca cgaaccactt caatacgttc gatggaatcg accgggatcc agttcagatc
    42301 gaaatcattg tggcggaaga cggcattgcg ggagttaacg cgtttaccgt cgacgagaat
    42361 cagggtgtag ctgctgtcca gaccacgaat actaacgccc ttacggttat ccccttcgtt
    42421 cgtcagttgt acgccaggca cttctttgag gacatccttc agattctgta ccggttttcg
    42481 ctgcaggtct tcctgggtaa tgacgctgat actggcgggt gcatctttga ggttttgttc
    42541 aacggaagat gcagtgacaa ccatcgtttc gccatcatca tcgaccgcta acacaggcca
    42601 tgcacaagaa atagcggaca aacacagccc gacccgtacg aaagggttca acctaaacat
    42661 tccatatctc catgaggtaa ctacgaaaat aaaatgggtt atcgctcaca tcttcttcac
    42721 gtccccttgc gtacggcagc atcgcggtgg acttatttgt atgcaggtga tcatcccaga
    42781 aagccggaac agcctctgat ttgtcagcct tttttgattg cggcgtaacg ataatgcaaa
    42841 cgataacaat tatcaatcca aatgttaaat tttatatccg catgatttgt cagggaaatg
    42901 gaattgaaaa agccctctcg gttgagaggg cttagcaagg aagggaggaa acttatttct
    42961 tatcgttctg cgggaacttc atttcgctgt agcgtacgaa gtgagttcct ttaatcagct
    43021 tgtagccgaa ccaaataatc aggaacagcg ggataccaat atacgttgcc gctacgccgc
    43081 cccagtcaat ggtatctttc aggaacgctt cgtagttctg gcccaaagtg ataatcagac
    43141 acagaatgaa tgcgaagatc ggccccagtg ggaagaaacc tgaacggtac ggcagatcgt
    43201 taatgtcgtg tccctgcaat acgtaaccgc gacggaagcg atagtggcta atggcaatcc
    43261 ccagccaggc gataaaaccc gtcatcccgg aggtgttcag cagccacagg tataccgtct
    43321 ggttgccaaa catggaggtc aggaagcaca gaccggcaat caccgtcgtc gcatacagcg
    43381 cattacgcgg cacgccacca cgcgacagtt tagcgaaaat gcgcggcgct ttaccgtcac
    43441 acgccagggt gtacagcata cgagtagacg catacatacc ggagttaccc gctgacagca
    43501 ccgccgtcag aataactgcg ttcatcaccg ccgccgcaga gagcagaccc gcgtgctgga
    43561 acaccagggt gaacggacta acgctgatgt ctttaacatc gttacgcagc aggctcggat
    43621 cggtgtacgg aataatcagg ctgataatca ggatcgcgaa cacatagaac aacaggattc
    43681 gccagaacac ctgacgtacc gcgcgtggaa tgtttttcgc cggatcttcg gactcgcctg
    43741 cagcaatacc gatcagctcg gttccctgga aagagaagcc gacaatcata gctacgccga
    43801 tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat tgtccagttg ctccagcccg
    43861 caggctgcgc gcctttgaag ataccgataa tcatcagcac gccaacgatg ataaagacaa
    43921 taactgtcgt gactttgatc agtgagaacc agtattccgc ttcaccaaag ccacgaactg
    43981 agatgtagtt cagcaggaag ataacgccga ggaacaacgc actccagatc cagcccggtg
    44041 tatccgggaa ccaccagctc atgaccagct gagctgcaac caggtcaacg gcgatagtca
    44101 ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc aaagccttct tcaacatagt
    44161 tctgaccgta agtggcaaac gaaccggaaa ccggcatata tgcagccagt tcaccgagac
    44221 tggtcatcag gaagtaaacc atcaggccaa tcagcatata cgagagcaat gccccgcccg
    44281 gacctgcctg agaaatcgtt gcgccagagg caacaaaaag acctgtaccg atggaaccgc
    44341 caatggcaat catcgtcagg tgacgcgcct ttaattcacg gcgtaagccc ggcgcttctg
    44401 tggttttagt ttcggaaacc atacaaaaat gctatccatc ttaaaaatga ggcgcgattg
    44461 tagcagacga ttggcaatcc ttccggcaga aatacacggt tataagacac cttcatgatc
    44521 gcccagggat tataagtaaa gcagccaatc tcttttctgg cgaatgcatg ctgagggcgc
    44581 tgcgtcacga aatcgacaca gcaccagcat gttcttgtac agcaacttaa cgcggtacat
    44641 tcgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt ggaaaggtgt ttttgccgat
    44701 gatgtatacg ccacaacgta cgcatcaggc gcgggagagg gaccgcaact tcacttaatg
    44761 tgcctgcctg caattgatct ttaatcaccc gtcgcgacag gcaactaatt cccaacccat
    44821 gacgcaccgc atgtttgatt gcctcggagt tacctaattc catcgccatc tcaaacttcg
    44881 gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt gccggaaccg cgttcacgca
    44941 ggatccacgg cgcagcggcc agctgttcta aggtgaccgg accacgagcc aacggcgaag
    45001 tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg ttcagaaatg atttcagtgc
    45061 tgtggcacgg tccttcaata aagccaatat caacgcggaa atccagcacc gcttgcatca
    45121 cgtcctggct gttcccaacg ctaagttcaa tcggcaactg cggataatca tggcgataac
    45181 gggcgataac tgcaggcaga atgtagttac cgatggtact actggcatag atacgaatcg
    45241 cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc ctgttcaagc aatgccaacg
    45301 cacgcggata gagcagccgc ccgtgttcat taacaaccag tcttttcccc acgcgatcaa
    45361 acagttgcac gccaagctgc ccttccaggt cggtcaaggc tgcgctcact gctgattgcg
    45421 acaacgccag catcaccgac gcctgggtgg ttgatccact tttcaatact tctgcaaaaa
    45481 cttccaactg ccggagggtg atgtgcatag tcgcttacca cttataaaga ttaattataa
    45541 atatataatc aattttattt ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag
    45601 agggttatga cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg
    45661 gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg
    45721 gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc
    45781 aacaccatct atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa
    45841 tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc
    45901 gatgtcggta tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg
    45961 gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt
    46021 gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagccggt agtgaaagcg
    46081 gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgctatt
    46141 ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga aactttcggt
    46201 atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc
    46261 agcccggatg cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct
    46321 ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag
    46381 aaaagcaaaa tcactattcc gtggtttgcc atcttgttca tcgtagttgc catctttaac
    46441 tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg
    46501 ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagcc
    46561 ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt
    46621 gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa
    46681 cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat
    46741 tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat
    46801 cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct
    46861 cacgacgcaa accatcgatg aattcaaagc ggcctgtgaa aaatatcact acacatcggc
    46921 gcaaattctt ccccacgaca gctatctgat taacctcgga catccggtca ctgaagctct
    46981 ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tggggctttc
    47041 tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc
    47101 gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtaa cagcggtgat
    47161 tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat
    47221 tatcgacggc gtggaagata aatcccgtgt cggcgtctgc attgatacct gccatgcttt
    47281 cgctgccggg tatgatttgc gtactccagc cgaatgcgaa aaaacgttcg ctgattttgc
    47341 ccgtattgtt ggctttaagt atctgcgcgg gatgcacctt aacgatgcga aaagcacctt
    47401 tggcagccgc gttgaccgcc atcatagcct cggtgaaggc aatatcggtc atgatgcgtt
    47461 ccgctggatc atgcaggacg accgtttcga cggcattccg ctgatccttg aaaccatcaa
    47521 cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt
    47581 ggcctgatcg atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct
    47641 gattcaacag aatgaaatcg ccgatattct gcaaatcagc cgctcatatt atggatttga
    47701 tgcgcaaggg tttgattaaa ggcaaaggat atatccttac cgagcaggat tactgtgtgg
    47761 ttgtgggtgc aatcaatatg gatattcgcg gaatggcgga tatccgttac ccgcaagcag
    47821 cctctcatcc aggttctgtt cactgttccg caggcggcgt cggacgtaat atcgcgcata
    47881 acctggctct gcttggtcgg gatgtgcatt taatatcagc tattggcaac gatttttacg
    47941 gcgagacatt actggaagaa acgcgccgcg cgggcgtgaa cgtttcaaac tgcattcgtc
    48001 tgcatggaca cagcactgca acttatctcg ctatcgctaa taagcaggaa gaaactatcc
    48061 tggcaatcaa tgacacacat attctgcaac agctaacgcc tcaactgctc aatacttcac
    48121 gggacttgat tcgccatgct ggtgtggtgc tggctgattg taatctcaca cctgaagccc
    48181 tggagtgggt ctttaccatt gctgatgaaa ttccgatgtt tgtcgatacc gtttccgaat
    48241 ttaaagcgaa caaagtaaaa aactggtaca gccggatcca cactctgaaa ccaacacaaa
    48301 atgaactgga aattttgtgg gggcaaccga ttaaagacga caacgatcgc atcagagctg
    48361 taaattcgct gcatcaacaa ggtgttaaac gcatttttgt ttatctgaaa gatgaatctg
    48421 tattttgcag cgataaggat ggagaacaat ttttgctgac cgcgccagcg catacgacag
    48481 tagacagttt tggtgctgac gatggtttta tggcgggcct ggtatatagc tttcttgaag
    48541 gatacagttt ccgcgacagc gcccgttttg cggtagcctg cgcggcaatt tcgcgcgcca
    48601 gcggcagctt aaacaaccct accctgtctg ccgataacgc gctttcatta gtgccaatgg
    48661 tgtaacaatg ttgccggatt cgacgcttga ccgcatctta tccggctcat aaattgcgca
    48721 ctacgctaaa ccaataaaga atcctgcaat agtagcactc atcaggttag aaagtgtcgc
    48781 cgccgccagc gcgcgtaaac caagttgggc gatttccggc gcacggtgtg gcgcaaccgc
    48841 agaaaacgcc cccaccacca ccccgataga accaaagtta gcgaaaccgc acaacgcgaa
    48901 agaaataatc gccacggttt tagcatccag agtgccaccc gtttgcagat atggcgagaa
    48961 attgagataa gcgacaaatt cattgatcgc cagcttctgc ccaatcaaac tcccggcaag
    49021 atttgcatca ctccagtcaa cccccatcac ccacgccaat ggggccaaca ggtaacctaa
    49081 aatggactcc agcgaggcat gtgcaaaacc aaaccagccg ccaacgccgc cgataatacc
    49141 attaattaac gcaatgatgg cgacaaatgc cataacaact gtcgctacac cggcggcgat
    49201 tttcagcccg gtcattgccc ctgtcgcagc ggcttcaata atgctttttg gcggtgtttc
    49261 ggtgaaagag aggttattaa aggaaacctg cgaagattcc gtagccgggc ttaacaggcg
    49321 ggcaaacaag atcccgcctg ggatcgccat taacgatgcc gccagcaaat attccacagg
    49381 tacgcccagg gcggcgtaac caatcattgt cgaaccagca atcgaggcca tgccactaca
    49441 aatcgctgta aataattcat tgcgattcag acgatcgata aagggtttga cgattgccgg
    49501 aatttcgttt tgcccgagga aaatggtagt gaccgcgacg aatgactaga tcttgctgat
    49561 atttaacgct ttctggaaga taccgccgag aatgcgaatt aaaatcccca tcacaccgat
    49621 gtagtagaga atactcacca gtgctgtaac gaagataatt gccggtaata ctctgaaacc
    49681 aaagataaat cctgcgccat caaacagcgt gtccattttc ggcccgacca gcgaaccgaa
    49741 gataaatgcg ctacccgcgt cgctgtacgc catcacttta tgcacgccaa aagcgacttt
    49801 ttcagcgacc caacgccctg gcggtaacca aagcataatg ccgccaatca caacctgtaa
    49861 cactaacgcc gcgccaacgg tacgcaggct gatcttcttc ttgtttactg acagcaaaaa
    49921 tgcaattgcc agcaatacca ccattcccag aacacttctc atgacatcca tagtgattat
    49981 ctcttcatgt catattgggc gacggctttc tgcccatcag aaatagccgg gcctgcatct
    50041 tgcgctatcg gcccggcttg ttatcaacgc ttaatgggtt ttgatgtagc cgcgcacgca
    50101 ctcttcgact aagccccaga accagtcggt atcaatagtg atgccgactt tggtattggc
    50161 gggcttgcca agaacgccca gctcgtcgca gacggtgcgc ccatagcaag ggccactgtt
    50221 gacgtccact tcgacgtaca tctcctgggt tttaatgcca tcagggttaa tcagataacc
    50281 gatgcaggtg gcgtcgtgca ccgggccgcc agccaggccg tagttttcga actgcgtttt
    50341 gagagtgaag ttcatgatgt cgctgaacag ctctccggca gggccgcctg ccctttccat
    50401 ccgagcaatc acgtccgggg tgcaaacggt ctggttggtg agatcgaggc ccatcatcac
    50461 taatggaacg ccagaggtga acaccacgcg ggctgcttcc gggtcagcaa agatgttgaa
    50521 ctcggcagat ggcgtgaagt tgcctgtacc gtaagcgccg cccattagca caatttcacg
    50581 gattttgggc aggatcgcgg gttgcatacg cattgccacc gcgatatttg aaagcggacc
    50641 aaccggcacc agagtgatat cgccatcgct tgccatcagg gtatcaatga tgtatttcac
    50701 cgcgtgggta ctttctgcct ggcgggttag cggctcgaat accgggccat ccagtccggt
    50761 ttcaccgtga atattatcgg caacgatttg ttgacgcata atgggttgcg gcatccccgc
    50821 ataaaccgga acattaatct ccagtttctg gcaaacattc aggccattaa ttaatgtttt
    50881 atcaagcgtc tgattacccg ctacaatagt gatgcctaat aaatctattg ccggatgttt
    50941 cgctgccatc atcattgcaa tagcatcatc atgacccgga tcacaatcca gaataatttt
    51001 tctcttttcc attgtttatt tcctctgttt ccagttgcgt tattttttct acagcaaaga
    51061 aaattcgacc gggccgatga ttgaatctta ccaaccgcgt acgtatgcta aatatgagaa
    51121 atctcatagc ggataaacat cgtgaaagaa atccacaata atgatcttaa gcagcaattg
    51181 atgagtgaat ccgcgtttaa ggattgcttt ttaacggatg tttcagccga tacgcggctg
    51241 tttcattttt tagcgcgtga ctacattgtg caggaagggc aacagccgtc ctggctgttt
    51301 tacctgacgc gaggccgcgc caggctttac gccacgctag ctaatggtcg cgtgtcgctg
    51361 atcgatttct ttgccgcccc ctgttttatt ggcgagattg agttaatcga caaagaccat
    51421 gaaccgcgtg cggtgcaggc tattgaagag tgttggtgcc ttgcgctccc tatgaaacat
    51481 taccgcccgc ggttattaaa cgacacgcta tttttacgaa aactctgcgt caccttaagt
    51541 cataaaaatt atcgtaatat tgtttcttta actcagaatc aatcatttcc gttagtgaat
    51601 cgcctggcag catttatatt actctcgcag gaaggtgatc tttatcacga aaagcatacg
    51661 caagcggcag agtatttagg cgtttcttat cgacatcttt tatatgttct cgcgcaattc
    51721 attcacgacg gtttattaat taaaagcaag aaagggtatc taattaaaaa cagaaagcag
    51781 ttgtcaggac tggcgctgga gatggacccg gagaataaat tctccgggat gatgcagtaa
    51841 aaattatttg caatagcgcg attgccggat gcaacactta ataagcgcgt acatccggca
    51901 atgaaactca agctaaacca ataaagaacc cggcaatggt cgcactcatc aggttggaaa
    51961 gcgttgctgc tgccagcgcc cgtaaaccaa gctgggcgat ttccggcgcg cgttttggcg
    52021 aaatagccga aaatgcgcca acgacaacac cgatagaacc aaagttagca aaaccacaaa
    52081 gcgcaaagga gataatcgca atggttttca cttccagcgt gccgcccgtt tgcaggtatg
    52141 gggagaaact caggtaagcg acgaattcgt taatcgccag tttctgccca atcaggctac
    52201 ccgcaagatt ggcatcactc cagtccacac ccatgatcca cgccagcggt gccagcacat
    52261 agccaaaaat actttccaga gaggcattgg cgaaaccaaa ccagccacca attccgccga
    52321 taatgccgtt gatcagcgca ataattgcga caaacgccat taccaccgtc gctacaccag
    52381 cggcgatttt cagcccggtc atcgcaccgc tcgccgccgc ttcgataaag ctttttggcg
    52441 gcgtttcgct gaacgacaga ttttcaaatg tgacctgtga aggctcggtg gccgggctaa
    52501 gaatacgtgc aaacaaaata ccacctggga tcgccatcag cgatgccgct aacaggtagt
    52561 caattggtac gcccattccg gcataaccaa tcatcatcga accagcaatg gacgccatcc
    52621 cgctacaaat tgcggtaaac aactcgttgc gattcatgcg atcgataaac ggtttaacga
    52681 tcgccgggat ctcattttgc ccgaggaaaa tagttgtgac tgcgacaaaa gattcgattt
    52741 tgctgatgtt aagagctttc tggaaaatgc tgccaaggat gcgaatcagc agccccatca
    52801 cgccaatgta gtacagcaga ctgatgagcg cagtaacgaa aataatcgcc ggaagtacgc
    52861 gaaaggcgaa gataaaaccc gcaccgtcaa acaggacatc cattttcggc ccaaccagcg
    52921 aaccaaaaat gaaggcgcta ccggcatcac tgtaagacat cactttatga acgcctaatg
    52981 ccgcctgttc tactgcccac tttcccggtg ggaagtagag catgatgcca ccaatagcga
    53041 tttgcagcag cagtgcggct ccaaccgtgc gcaaactgat gctcttttta ttcactgaca
    53101 acagaaatgc tattgccagt aacaccacca tccccacaac gcttctcatt atatccataa
    53161 tgattttccc ttcatgccag taaacccggc gtcagcgcca ggttttggta tgcttgatga
    53221 gtacgggcga cggctttctg cccgtcagaa attaacccgc gagacgctgg tattctttgg
    53281 caatttcact cgccagaatg gcgttgttga acaccagctg gatattggat ttcaggctgt
    53341 caccgccggt cagctcagca acgcgcgcca gcaggaacgg tgtactttct ttaccaataa
    53401 cgccctgctc ttcagcttcg gcgacggctt gatctatggc tgcattgata gattcctccg
    53461 gcatcgcaaa ctgttccggg atcggattcg ccactaccag gccgccgttc aaaccggttt
    53521 gccatttcac tgccattgca cgagcgattt ccgtggcact atcaaggcga atactgactt
    53581 caaatgggct ggtacggcag aaaaacgcag gcagcgcttt tgtctgatag ccaattaacg
    53641 gcacaccgaa ggtttctaaa tattctgttg ttaatcctaa atcgagaata gatttcgccc
    53701 cggcacaaac aacggtgaca ttagtatttg ccagttcttg caaatcggca gaaatatcga
    53761 aggtatgttc cgccccgcga tgcacaccac caattccccc ggtggcaaat actttaattc
    53821 cggcaagcgc cgcaataatc atcgttgaag ccacagtggt tgcgccattt tttccggcgg
    53881 caacaacaaa aggtaaatcg cgacgactaa ctttggtcac gttatgccct tcacgaccca
    53941 gtaattcaat ttcttctttg cttaaaccca ctttcatcac gccgccaata atggcgatag
    54001 tggcaggtac tgcgccctgt ttacgaatag tttcttctac ttcaattgcg gtctgggcat
    54061 tttgtgggaa cggcatcccg tgagaaataa tggtcgattc cagcgccaca accggttttt
    54121 tgttttttaa agcgtcctgc acttccgggg aaatttgtaa taattcaggg gaaattttta
    54181 attcagacat tctgcgttct ccactaacga tataacgttg gcaatcgata aatcggggtt
    54241 attggtgtat tcacaggaga gcgccattga cgaacatccc tgtgcgaaac gaacagattc
    54301 ggcaaacggc attccgtcta cccaacacga agcaagtccc gccatcatgg catcgcccgc
    54361 tccggtaaca ttaataacat tggttttgat cggggcagac cagccacttt caccgctgat
    54421 atcactgtaa taaacgccat cgccgcccat gctcaatacc agtcggttca ggccatgttg
    54481 atggaaccag gcagcaactt ttgccacatc tccacgccct gacagcgcaa tcccactcag
    54541 tgtttccgct tcaaggcggt ttggcttcag agtgtggatc tgatttagac ggtcgcgcac
    54601 tttgacacat ttccacgctg aaaccggatc gacaaatacg ggtacgttgg cagcattatc
    54661 cagaatccat gccagtgcct cttcactgat attacagtcc gcgacaatga cctttgccct
    54721 ctgaataaat tcacggtgct gtgcgagata ttcagctgta atagcgttgc taatattcat
    54781 gtcatttata gcaaccagca tttcaccggt attatcgagt aatgataaat aactcgacgt
    54841 attttctccc ggcacaatca ggcatttatc gacataaacg ccagattgat tggtttgcgt
    54901 tagcagcgat tgaccataaa aatcactacc tactgcgctc agtaaccagg ctttgttacc
    54961 cagcaacgcc aggttttgtg caatatttcg ccctactcca ccaggtgtaa attttatttt
    55021 acctggattt gaatccgcat aatttaatga ttcatgtgaa tatccggcga catcaatatt
    55081 cgccgaacct ataattacga cataatcctt ttcgcgcata gcacgtcctc tggtaaaaaa
    55141 ttaccaaccc tgtcaatcca caagaatata gatactgcaa agacagttta aacatgtgtt
    55201 catatttaaa catgtgctca tggtatgcat gttttacgta aagtaaattg ccgccagaga
    55261 ggcaaagcgg gcagatttga gcaggctcac aaaatgcgat taaatgccag aattacaaaa
    55321 gcgaggaatt tgtgacggga tgcacagaac aggaacacta aaaaagggca gaaaagaatc
    55381 tgcccttgag gaaataacct tagtgtttga tgaacgtttc aggtttctac tgaaagataa
    55441 aaccatttaa attcataaaa ttacattcac aaaccggtca ttctctttgc gctcatccaa
    55501 tgatacaggg cagtaaattt ctgccctgta acacaccttt tattacgcgg ctttcgctac
    55561 tgcgtccact tccggacgtt tcaggaaggc ataggccaaa cccgccacca gcgtaccggc
    55621 aataattgct accaggtaac ccagtaccgg cgtaatagcg ccagggatca gcagaacaaa
    55681 cagaccaccg tgcggtgcca tcagtttcgc accaatcgcc attgagattg cgccagtcag
    55741 cgccccacca acgatacaac acggcagcac acgcatcgga tcacgagcag caaacggaat
    55801 tgcaccttcc gaaatgaagc acagtcccaa taccagagcg gctttgccac cttcctgctg
    55861 cgctttgtcg aatttgcgac gcgccaccat tgttgccaga cccattgcca gcggtggcac
    55921 catacctgcc gccataatcg ccgccatcgg gccataggtt tgagtactca gcagacccac
    55981 accgaatgcg tacgctgctt tgtttaccgg accgcccatg tcagtacaca tcatgccacc
    56041 gaggatcgcc cccagcagaa ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa
    56101 cccttcgaga atgccagcaa ctggtttacc gatcaggtag atcatcgcca gaccgaccac
    56161 cagactggaa attagcggaa tgatcaggat cggtttcagc gcctccatac tctgtggcag
    56221 tttcagttgc gtactgatta acttcgcaat gtaaccagcc aggaagcccg caataatacc
    56281 gccaatgaag ccagaaccgg tgctgaccgc cagcataccg ccaatcagac ccggcgtgag
    56341 acccggacga tcggcaatgg agaaggcaat gtaacctgcc agtaccggca ccatcagcgc
    56401 aaaggctgaa ccaccaccaa tctgcatcag cgccgctgcc aacgtacccg gctctttaaa
    56461 cgcttcgata ccaaaagcaa aagaaagcgc gatacacaga ccacctgcaa cgaccatcgg
    56521 cagcatgtaa gagacgcccg tcagcaagtg acggtatgcg cctgcactct ctttcttacc
    56581 ttcagaggtc gccgtttgag ctttgcccgc cggttcatac ggcgttgctt cagcaaccgc
    56641 tttatccagt tcctgcgcgg ttttcttcag cgccagaccg gtagaggtac gatacatcgg
    56701 tttaccagca aatttcgcca gatccacttc gatatctgcc gccacaatca ccagatccgc
    56761 tgcggctact tcttcggggg tgattgcatt acccgcacca acagaaccac gggtttcaac
    56821 tttcacccac cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt
    56881 gtgtgctacg ccagtcgggc aagcagtcac cgcaactacg cgtttcggac cgctggcggc
    56941 aaccggtgct gtcgcagcga ccggcgcagt gtaaggtttc gcatggcctt tggcttcact
    57001 caggaacagc tcagggtgcg caactgcccg ggaaatatca cccagccaga catttttacc
    57061 gttcagcgcg ctgtcattcg ggatggaatc accgagaaca atcgccattt ctgcgtcgtt
    57121 cggattgtcg atgatttcca gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc
    57181 catataggcg cgtgcctgac cgagattagc gtcaataatc agcagcgttt tcattatgcc
    57241 tctcctgctg tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg
    57301 gacgatcggt aatacccaca ttgctttgac ttaccgccag ggctgcaaca gcagtcgcca
    57361 gacgcagtgt gtgttcactg gattcacgca tcagcaagcc ataaatcagg ccaccaacca
    57421 tagaatcccc tgcgccaacg gtgcttacga catcgactga cggtggtttg gcgatccatt
    57481 cgccggaggc attaacccaa agcgcgcctt cggcacccag tgaaataaca acatgcgcaa
    57541 taccttgttc acgtagcgca tgtgcagctt caatcacatc tttcatttca ggcagtttac
    57601 ggcctgccca gatttccaac tcgcggcggt taggtttcac cagccacggt gccgctttca
    57661 aacctgctac taacgcttca cggctactat caaagataat gcaaggacac tgactacgca
    57721 ggcgagtcat ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag cttccgctga
    57781 cacagaccat atcgaactga ccgagccagc tcagagaatc agtcacaaag cgttcccagt
    57841 cggcgggggt gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg tctttttccg
    57901 tcagcttaac gttaattcgg gtgcgcccct gtacaacctg gaaacggttg gcaatgccca
    57961 gctcgctgaa cagttgctga aaaccatcct gattgtcttt acccaggaag ccgccaacgg
    58021 tgacatcaat tcccaggtct tttaatactt tggccacgtt gatgccttta cccgccgcat
    58081 gcagaccggt ggttttcacc aggttcactt cgccgcgttc aatttccggg cagaaaccaa
    58141 caaggtcata agccggatta agggtgatag tagcaacacg tctgctcatt atgcgccctc
    58201 cccaagacca gcagcgatag cgtcgccgat tgctttcagc gcctgttcag catctgcacc
    58261 ctgggcggta aagcgtaggc gatgaccttt cttaacgcca agtgccacaa ctttcatcag
    58321 actacgtccg tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt
    58381 aaattgttta atggtgttga ccagcatggt acctggacga gcatgcaggc cgtgttcatt
    58441 gcgcaccaca aactccgcgc ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag
    58501 cgccagcaac gttgccgcat ccgctttcag caagcggtca gctttattgt cgagcaacaa
    58561 atcagcgaga cgcttaagaa ccgcaatggg ctgatcgtca ttcatcgcca cactcaccag
    58621 catggctgcc gtttcgccgt ccacatcaaa agcatttgcc gcacggctta ccgcaatcgc
    58681 gctacgcaga ttgccttcgg cgctatcgct cagccagata ccctgtccga ggttcagcgg
    58741 ttgttcattg atggctttgg tgacgaaagt ggcgtcaact gccctcgcct ctttcagacg
    58801 cgcagcgttc agcgcctgaa gagtcagcag atcgctggcg gcgatatcca gcgtcagcat
    58861 ttcgttgtcg agcttcagct gctcactctg cttttcgccc atcagcaatg cgcgaagttc
    58921 ttctgctgtt gttgctgact tcagttgttc agcaacggaa tcatcgctca gtacgtgggt
    58981 cagctggcgt agcaggccca gatgttcatc cgagctggca gcaataccga tcgctacgta
    59041 cgctacctga ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct gaacgccggt
    59101 tttcagcacc tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac cattgccgag
    59161 gaacgttgag gtttgctgtt cgcgcgccag catgccattg acgtagcctt ctgctacgtt
    59221 accggcctgc accagcgccg cagcgagctg gcgaatcgcc tcttctttgt ctccggcctt
    59281 ttcgcccgga tggatgtcct gtacggataa ctggaacata gttctcctct cttgctgaat
    59341 tgaaacgatt cagcttctat gagaaaaaaa gcgccaacct ggcttagggt taaagacaag
    59401 atcgcgctga aacgtttcaa gaaagcataa tacttctgtt tcagcacgca aggaaagccg
    59461 taaagttatg agcaaaagtt tgatgtgctg cacaattttt tgtcattttt cctaattgct
    59521 gatgggaaaa tccgttatca gcaatttcat ttcagcagcg tcagcagcca atcagcaaca
    59581 gtgaaatggc attttgatta ttcgtggcgc ttttgatttc gctgtctgtt tattttctga
    59641 caagcagcgt aaactccgcg tcttcctctt ccagtgatcg accagcatgc ataactcccc
    59701 cgcagtctcc agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt
    59761 tctcaccggt attgcgggcg ctctgcaaac cccgacactc agtatttttc ttaccaatga
    59821 agtacatgcc cgtccggcga tggtgggatt cttctttacc ggcagcgctg tcattgggat
    59881 tctggtaagt cagtttctcg ccgggcgctc tgataagcgc ggcgatcgca aatcgctgat
    59941 tgtcttttgc tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta
    60001 ctttgttttg ctattcgttg gcgtctttct tagcagcttt ggctcgaccg ctaacccgca
    60061 aatgtttgcc cttgcccgtg aacatgccga caaaaccgga cgtgaggcgg tgatgttcag
    60121 ctctttttta cgcgctcagg tttcactggc atgggtcatt ggcccaccgc tggcttatgc
    60181 gttagcgatg ggtttcagct ttacggtaat gtatctgagc gcagcggtag cgtttattgt
    60241 ttgcggcgtg atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac
    60301 cggcacggtt gaagcgccgc gccgtaaccg tcgcgatacg ctactgctgt ttgtcatttg
    60361 tacattaatg tggggctcga acagcctgta catcatcaac atgccgctat ttattatcaa
    60421 cgaactgcat cttcccgaga aactggccgg tgtgatgatg gggaccgccg ccgggctgga
    60481 aatcccgacg atgttgattg ccggatattt cgccaaacgt ctgggtaagc gtttcttaat
    60541 gcgcgttgct gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg cgcattcacc
    60601 tgtcattctg ttgggcttgc agctgctaaa tgctattttt attggcattc tgggcggcat
    60661 cgggatgctc tattttcagg atctgatgcc cggtcaggcg ggttcagcca ccacgctcta
    60721 taccaacact tcgcgcgtgg gatggatcat cgcaggatca gtggcgggca tcgtcgccga
    60781 gatctggaat tatcacgctg tgttctggtt tgcgatggtg atgattatcg ccactctgtt
    60841 ttgcttactg cggattaaag atgtttaagg cgcggtcagc atttccagat cgataagcca
    60901 ggtcatggcc tgctgacgcg agttaccgca catttcagcg ctggcttgta agcctgcaca
    60961 gactttcggt cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat caagctgaat
    61021 gcagggcgta ttggcgggct tgccatctgg cataccggga atagggctgg aaattgaagg
    61081 ggcagtgcaa cacgccccgc aacccggacg gcattccata cgttgttctc ttaaggttaa
    61141 ccaccggatt gatgagcgcg cacagtacca ccttttttgc accagcaaaa gtgcgaatac
    61201 cacttgcccg aaaggcccgt cgcgagtact ttgtcgcgat atttttgaca ttttcgacta
    61261 caggaatttt tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac tgaattacaa
    61321 cggcaaactg ctgctggtta aggatattga tattcagtcg cccactgccc gcggtgccgc
    61381 tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt
    61441 caaaggtgat gatatcgttg acaccgtgac gctgacccgc cgttacgttg acttctccta
    61501 tgtcgatggc aacgaatatg tctttatgga taaagaagac tataccccgt ataccttcac
    61561 caaagatcag attgaagaag agttgctgtt tatgccagag ggcggcatgc cggacatgca
    61621 ggtgctgacc tgggacggtc aactgctggc gcttgagctg ccgcagaccg ttgatctgga
    61681 aatcgttgaa accgcaccag gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc
    61741 gacattgagc actggtctgg tgattcaggt accagaatac ttaagcccgg gcgaaaaaat
    61801 tcgtatccat atcgaagaac gccgttatat ggggcgtgct gactaacttc agccgcatgc
    61861 agaaaaggga tagctcaggc tgtccctttt ttaatttatt ataccaatct tctattttgc
    61921 gcttcctgaa caccttacgc cctcaatttt cactcgttga ttgatcgccc tcacacttca
    61981 tcgcattaac aatccagacc aatttcaatt gctgtcatat aactttacac tgtcattgtt
    62041 aatttatcgt tactaagacg tgactcctat gaagacaatt gcctccgtta cgctcccgca
    62101 tcatgtacac gctccacgct acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg
    62161 ctttggcgcc tttcaccgcg ctcatcaggc gttactgacc gatcgtgtgc tgaatgccca
    62221 gggcggcgac tgggggatct gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca
    62281 gctccgcgca cagaaccatt tatataccgt gctggagaaa ggtgcggacg gcaatcaggt
    62341 gataattgtc ggtgccgttc acgaatgcct taatgcaaaa ctggattcct tagcggcaat
    62401 tattgagaaa ttttgcgagc cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg
    62461 ctattgtatt gacccggcca ccggtgcact cgacaccagt aatccgcgga ttattcacga
    62521 tctacaaacc cctgaagaac ctcactccgc accgggtatt ctcgtcgaag cactgaaacg
    62581 ccgccgtgag cgcggcctta caccgtttac cgtgctctcc tgcgacaata ttcccgacaa
    62641 tggtcatgtg gtgaaaaacg cggtgctggg aatggcagaa aaacgttcgc cagaactcgc
    62701 cgggtggata aaagagcacg tcagttttcc gggaaccatg gtcgaccgca ttgttccggc
    62761 tgcaaccgac gaatcactgg tggaaatcag ccagcatctg ggggtgaatg atccctgcgc
    62821 gattagctgc gaaccgttta tccagtgggt ggtggaagat aacttcgtcg ctgggcgtcc
    62881 tgcctgggaa gtcgcagatg tacaaatggt gaatgatgtc ctgccatggg aagagatgaa
    62941 actgcggatg cttaatggca gccactcttt tctcgcttat ctgggttacc tctcaggatt
    63001 cgcccatatc agtgattgca tgcaggatcg cgcatttcgc catgccgcca gaacattaat
    63061 gctggatgag caagcgccga cactgcaaat taaagatgtc gatttaacac aatatgcgga
    63121 taagttaatt gcacgttttg ctaatccggc gctgaaacat aagacctggc aaatcgcgat
    63181 ggatggcagc cagaaattac cgcaacgcat gctggcaggt attcgcatac atcaggggcg
    63241 cgaaacggac tggtcgttgc tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg
    63301 cgttgatgat gccggaaatg ccattgatgt tcgcgatccg cttagcgata aaattcgcga
    63361 acttgttgcg ggcagcagca gtgaacaacg cgtaaccgcc ctgctttccc tgcgtgaagt
    63421 tttcggtgat gatctgccag ataacccgca ttttgtgcag gccatcgaac aagcctggca
    63481 acaaatcgta caattcggcg cacatcaggc gctattaaac accctcaaaa tttaacgatt
    63541 tctgcggtta aagcggatga agctcacctt cgtccgctct ccccttctct tttctgcttt
    63601 ttttagccag gattaacgct cagttaactt accagagtat tgcggagccg ttgtgaccag
    63661 gaccaacctc atcaccggtt ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt
    63721 agcccataaa gatcccaacg aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg
    63781 aattgatggt gctttgctcg ccgatagcgg cgcattgctg aaagagatcc ccggcggctg
    63841 catgtgctgc gttaatggtt tacccatgca ggtagggttg aataccttac tgcgtcaggg
    63901 aaaaccagac cgcttgttga tagagccgac cgggctgggc catccgaaac agatcctcga
    63961 tctgttaacc gcaccagtct atgaaccgtg gatagatctg cgcgccacct tgtgcattct
    64021 cgatccgcgc ttgctgctgg acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct
    64081 ggctgccgca gacatcattg tcgccaataa atccgaccgt gcgacgcccg aaagtgagca
    64141 agcgctacag cgttggtggc agcaaaatgg tggcgatcga caattaattc acagtgagca
    64201 tgggaaagtt gacggtcatc ttctggattt gccgcgtcgc aatttagccg agttgcccgc
    64261 cagcgccgcg cattctcatc agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc
    64321 agagcatcaa cgctggcgtc gcagtctgaa cagcgggcaa ggatatcagg cctgcggctg
    64381 gatattcgac gctgatacgg tattcgacac cattggcatt ctggaatggg cgcgacttgc
    64441 accggtggaa cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtac gaatcaaccg
    64501 tcagggcgat gacctgcaca ttgaaacgca aaacgttgcg ccaccggaca gccgtattga
    64561 gctgatttcc agcagcgaag ctgactggaa tgctttgcag agcgcgctgt tgaagcttcg
    64621 tttagcgact accgcgtaag gttgcctgcg tttttcagta agataattag agaaaatatg
    64681 attaaaaatt tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct gtttctttcc
    64741 tggtatattc ccgttaatca tggattctgg ttgccgattg atgcggatat tttttatttc
    64801 tttaatcaga aactggtcga aagtaaggcc tttttgtggc tggttgcatt gaccaacaat
    64861 cgcgctttcg acggttgttc tctgctggcg atgggaatgt tgatgctgag tttctggctg
    64921 aaagaaaacg cccctggcag acgacgtatc gtgattatgg gtctggtcat gctattaact
    64981 gcagtggtat taaaccagct gggtcaggca ttaattcctg taaaacgggc cagcccaaca
    65041 ttgactttta ccgatattaa ccgcgtcagc gaactgctct ctgttcccac gaaagatgcc
    65101 tcacgagata gctttcccgg cgatcacggc atgatgctgc ttattttttc ggcattcatg
    65161 tggcgttatt tcggcaaagt tgcaggcctt atcgccctta ttatttttgt ggtttttgca
    65221 tttcccagag taatgattgg cgcacactgg tttactgaca tcattgtcgg ttcgatgacc
    65281 gtgatattga tcggtttgcc ctgggtgttg ctgacgccat taagtgatcg attaatcacc
    65341 ttttttgaca aatcactacc gggaaaaaac aaacatttcc aaaacaaata actcacagta
    65401 attaacatca tcaggcttat ttttatagtg aggataatcc tgatgatgcg caccgtgctt
    65461 tcatccatcg cgcgcaaaaa tcattctcta agtaaatgaa tggattgcat gcgtttcact
    65521 caatcgtact ttaattgacc aatcccgctt attaactttc tgtatcactt tttcttataa
    65581 aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg gtaatctcga actcgttttg
    65641 cctcggcggt agattatcct cacagcatat aattttgtgc gttagtccac agatttggcc
    65701 ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc atttttttat
    65761 aacgatattt gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa atctcaaccg
    65821 attttgagat atatcttgcg cgggatcccc gcgattgcag tagcggttct gctttctgca
    65881 tgtagtgcaa ataacaccgc aaagaatatg catcctgaga cacgtgcagt gggtagtgaa
    65941 acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc tggttcgtaa tgtcgacgta
    66001 aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg tacgttatcg tctgggcggc
    66061 agcactaaaa aaggtatcga ttgttctggt tttgtacaac gtacattccg tgagcaattt
    66121 ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa tgggtaaatc tgtttcccgc
    66181 agtaatttgc gtacgggtga tttagttctg ttccgtgccg gttcaacggg acgccatgtt
    66241 ggtatttata tcggcaacaa tcagtttgtc catgcttcca ccagcagtgg tgttattatt
    66301 tccagcatga atgaaccgta ctggaagaag cgttacaacg aagcacgccg ggttctcagc
    66361 cgcagctaat aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt aactgaaagc
    66421 actgcttagg cagtgctttt ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt
    66481 gatccaggct atagtccggt cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt
    66541 aacggaatca tgttcatacg cgctcccaat tctggacgta agctcctgct tacctgcatt
    66601 gttgcaggcg tgatgattgc gatactggtg agttgccttc aatttttagt ggcctggcat
    66661 aagcacgaag tcaaatacga cacactgatt atcgacgtac aaaagtatct cgatacctat
    66721 tttgccgacc tgaaatccac tactgaccgg cttcagccgc tgaccttaga tacctgccag
    66781 caggctaacc ccgaactgac cgctcgcgcg gcgtttagca tgaatgtccg tacgtttgtg
    66841 ctggtgaaag ataaaaaaac attctgttca tctgcgactg gtgagatgga cattccactc
    66901 aatgaattga ttccggcgct cgacattaat aaaaacgtcg atatggcgat cttacccggc
    66961 acgccgatgg tgccgaacaa acccgcaatc gtcatctggt atcgcaaccc tttgctgaaa
    67021 aatagcggtg tgtttgccgc tctgaatctc aacctgacgc cttctctctt ttatagttca
    67081 cggcaggaag attacgatgg cgtcgccctc attattggca atactgcgct atctaccttt
    67141 tcttcacgtt tgatgaacgt taatgaatta accgacatgc cggtccgtga aactaaaatt
    67201 gcgggcattc ctctgaccgt tcggctttat gcggatgact ggacatggaa cgatgtgtgg
    67261 tacgcatttt tactgggcgg catgagtgga actgtcgttg gcctgctctg ctattacctg
    67321 atgagcgtac gtatgcgccc cggcagagaa atcatgaccg ccatcaagcg cgaacaattt
    67381 tacgtggcgt atcaaccggt ggtggataca caagctttgc gagtaacggg cctggaagta
    67441 ctgctacgct ggcggcatcc tgtcgcggga gaaattcccc cggatgcctt cattaacttt
    67501 gccgaatcgc aaaagatgat tgtgccgctg actcagcacc tgtttgagtt aattgcccgc
    67561 gatgccgcag aattagaaaa agtgctgcca gtaggcgtca aatttggtat taacattgcg
    67621 ccggaccatc tgcacagcga aagctttaaa gcagatatcc agaaactgct cacttcccta
    67681 cccgcacacc atttccagat tgtgctggaa attaccgagc gcgatatgct gaaagagcaa
    67741 gaagccacac aactcttcgc ctggctgcac tcggtcggcg tagaaattgc tattgatgac
    67801 ttcggcaccg ggcacagcgc gcttatctat cttgagcgtt ttacgctcga ttatctgaaa
    67861 attgaccgtg gatttatcaa cgccatcggt acggaaacga tcacttcacc cgtacttgac
    67921 gcggtgctga cgctggcgaa acgtctcaat atgctgacag ttgctgaagg ggtcgaaacg
    67981 ccggaacaag cgcgatggct aagcgaacgc ggcgttaatt tcatgcaagg ctactggatt
    68041 agtcgcccgt taccgctgga cgattttgtc cgctggctga agaaaccgta tacgccgcag
    68101 tggtaaggtg tgcttacgtc ccttattatt catagtgaaa gcatgccgga ttgcggctaa
    68161 tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca tactgctgct gtttatcgct
    68221 ctgttcacct ttggtgtgca ggcgcaggct atcaaggaaa gctatgcctt tgccgtgctg
    68281 ggcgaacccc ggtacgcgtt taatttcaac cattttgatt atgtgaaccc cgccgcgcca
    68341 aaaggtgggc agataacgtt gtcagccctc ggcaccttcg ataatttcaa ccgctatgca
    68401 ctgcgcggca acccgggcgc acgcaccgag cagctgtacg acacgctatt tacgacttcc
    68461 gatgacgaac caggcagtta ttacccgctg attgctgaaa gcgcacgcta tgctgacgat
    68521 tattcctggg tggaggtcgc tattaatccg cgcgcccgtt ttcatgatgg ttcgcccatt
    68581 actgcccgcg atgtagagtt tacttttcaa aaatttatga ccgaaggcgt gccgcaattt
    68641 cgtctggtct acaaaggcac caccgtcaaa gccattgcgc cgttaaccgt gcgcattgag
    68701 ttagctaaac ccggcaaaga agatatgttg agtctgtttt cgctgccggt atttccagaa
    68761 aagtactgga aggatcacaa acttagcgac ccgctcgcca cgcctccgct tgccagtggt
    68821 ccgtaccgca ttacgtcctg gaaaatgggg caaaatattg tctattcccg cgtgaaagat
    68881 tactgggcag caagcttacc ggtaaaccgc ggacgctgga atttcgacac cattcgctac
    68941 gattattacc tcgatgataa tgtcgccttt gaagcgttta aagcaggtgc ctttgatttg
    69001 cgtatggaaa acgacgccaa aaactgggcc acgcgttata ccggtaaaaa tttcgataaa
    69061 aaatacatca tcaaagatga gcaaaagaac gaatcagccc aggatacgcg ctggctggcg
    69121 tttaatatcc aacgtccagt attcagcgat cgccgggtgc gggaagcaat cactctcgcc
    69181 tttgactttg aatggatgaa caaggcgttg ttttacaatg cctggagtcg cacgaacagt
    69241 tattttcaga ataccgaata cgcggccaga aattaccccg acgccgcgga gctggtgctt
    69301 ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca cacaaatcta ccagccgccg
    69361 gtatccaaag gcgatggcta cgatcgtgac aacctgttaa aagccgacaa acttctcaac
    69421 gaagcgggct gggtgctgaa gggtcagcaa cgcgttaatg ccacaacggg tcagccactc
    69481 agctttgaat tattgcttcc cgcaagcagc aatagtcagt gggtattgcc gttccagcac
    69541 agcctgcaac ggctgggtat caacatggac attcgcaagg tggataactc gcaaatcact
    69601 aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcg tatggcgggc gatgccgtgg
    69661 cccagttccg atttacagat ttcctggtca tcggaatata tcaattccac ttataatgcc
    69721 cccggcgtgc aaagcccggt tatcgactcg ctgatcaacc aaattattgc cgcgcaggga
    69781 aataaagaaa aattactgcc gttggggcga gcactggatc gcgtattaac gtggaattat
    69841 tacatgctgc caatgtggta catggcggaa gaccgtctcg cctggtggga taaattctcc
    69901 cagccggccg tgcgccccat ctatagcctc ggtatcgata cctggtggta tgacgtcaat
    69961 aaagcggcca aactgccgtc cgccagcaaa cagggagagt agatgggcgc ttacctgatt
    70021 cgccgtctgt tgctggtgat cccaacatta tgggcgatta tcaccatcaa ctttttcatc
    70081 gtgcaaattg cgcctggcgg tccggtcgac caggccatcg ccgccattga gtttggtaat
    70141 gccggagtat tacccggcgc aggcggtgaa ggtgttcgtg ccagccatgc gcaaacgggt
    70201 gtcggcaata tcagcgacag taagtaccgt ggcggacgcg gattagatcc agaagtgatc
    70261 gctgagatca ctcatcgcta cggttttgat aagccgatcc acgaacgtta cttcaaaatg
    70321 ctctgggact acatccgctt tgattttggc gatagcctgt ttcgcagcgc ctcggtgctg
    70381 acgctgatta aagacagtct gccggtttcc atcaccctcg gattgtggag cacgctgatt
    70441 atctatctgg tgtcgattcc gttaggcatt cgcaaagctg tttataatgg gagccgcttt
    70501 gacgtctgga gtagcgcatt tatcatcatc ggctacgcca ttccggcctt tttgtttgcc
    70561 atcctgctga ttgtcttctt cgcgggcggc agctatttcg acctgttccc tctacgcggc
    70621 ctggtttccg ctaactttga ttcgctgccg tggtatcaga aaatcaccga ttatctgtgg
    70681 catatcacgc tgccggtgct ggcgacagtg attggtggct ttgcggcgct gaccatgctg
    70741 acaaaaaact cattccttga tgaagtgcgc aagcaatacg tggtgaccgc gcgtgcgaaa
    70801 ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc gcaacgccat gctgctggtg
    70861 attgccggtt ttccggcgac gtttatcagc atgtttttta ccggctcgct gctgattgag
    70921 gtgatgtttt cactcaatgg tctgggctta ctgggctacg aagcgaccgt ctcgcgcgat
    70981 tatcctgtaa tgtttggtac cttgtatatt ttcaccctga ttggcctgct gctgaatatt
    71041 gtcagtgata tcagctatac gctggtcgat ccgcgtatag attttgaggg acgttaatgt
    71101 cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt tcgccataac cgtcgcggct
    71161 actggtcgtt atggattttc ctcgttttgt ttggtttgag tttgtgttct gaacttatcg
    71221 ccaacgataa accgttgctg gtacgttatg acggcagttg gtatttcccg ttgttgaaaa
    71281 actacagcga aagcgatttt ggcggcccgc tggcaagtca ggctgattat caggacccgt
    71341 ggctgaaaca acggctggaa aataacggct gggtactgtg ggcaccgatt cgctttggtg
    71401 ctaccagtat caactttgct accgataagc ccttcccttc tccaccctcc cggcaaaact
    71461 ggctgggaac ggatgccaac ggcggcgatg tgctggcacg tattctctat ggcacgcgga
    71521 tctcggttct gtttggcctg atgctgactc tctgttccag cgtgatgggc gtgctggcgg
    71581 gggcgctaca aggctattac ggcggtaaag tcgatctctg ggggcaacgc tttattgaag
    71641 tatggtcggg gatgccgacg ctgtttttga ttattttact ttccagcgtc gtacagccta
    71701 acttctggtg gctgctggca attactgtct tgtttggctg gatgagtctg gtcggcgtgg
    71761 tgcgggcgga gtttttacgc acccgtaatt tcgactacat tcgtgcggcg caggcgcttg
    71821 gcgtcagcga tcgcagtatc atcctgcgtc atatgttgcc taatgccatg gtcgcgaccc
    71881 tcaccttttt accgtttatt ttatgtagtt cgataaccac cctgacctca ctcgatttcc
    71941 tcggcttcgg tctgccgctc ggttcaccgt cactcggtga actgctgtta caagggaaaa
    72001 ataaccttca ggctccgtgg cttgggatca ccgccttctt gtcggtggcg atattattgt
    72061 ctttgctgat ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt
    72121 agcatgacgc aaactctgtt agcgattgaa gatttgtcgg tgggttttcg ccatcagcaa
    72181 accgtacgta cagtagtcaa tgatgtttca ctacagattg aggctggcga aacgctggcg
    72241 ctggtgggtg agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc
    72301 ccttccccgc cggttgaata tctctccggc gatattcgtt ttcgtggcga atcgctgctt
    72361 catgccagcg atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcaa
    72421 gagccgatgg tgtcgttaaa tccattgcat accctggaaa aacagcttta tgaagtgctt
    72481 tcactccacc gcgggatgcg tcgggaagcg gctcgaggcg aaattcttaa ctgccttgat
    72541 cgcgttggta tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc
    72601 ggcgaacgcc agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt
    72661 gccgatgaac cgaccaccgc actggacgtc tctgtccagg cgcagatttt acagctgttg
    72721 cgcgaactgc aaggcgagct gaatatgggc atgctgttta ttactcataa cctcagcatt
    72781 gtcagaaaac tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat
    72841 aacgccgcta cgctatttgc ctcccccact catccttaca cacaaaagct actcaacagt
    72901 gaaccgtcag gcgacccggt gccgttgcca gaacctgcct caacgttgct ggatgttgaa
    72961 cagcttcagg ttgccttccc cattcgcaaa gggattttga agcgcattgt ggatcataat
    73021 gtggtggtga aaaacatcag ttttacgcta cgagcgggtg aaacactggg tttagtgggc
    73081 gagtccggtt ccgggaaaag tacgacggga ctggcgctgc tgcgactgat taattctcag
    73141 ggcagcatca tctttgacgg tcagccactg caaaatttaa atcgccgcca gctgttacct
    73201 attcgtcatc gcattcaggt ggtatttcag gatccaaact cctcgctcaa cccacgactc
    73261 aacgttttgc agattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca
    73321 caacgcgaac aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc
    73381 caccgttatc cggcggagtt ctctggtggt cagcgacaac gtattgcgat tgccagggca
    73441 ttaattctta agtcctcgct gatcatactt gatgaaccga catcatcact cgacaaaacg
    73501 gtacaggcgc aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat
    73561 ttgtttatca gccacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg
    73621 cgacaagggg aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccac accgcagcag
    73681 gagtatacgc gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa
    73741 gggttcggca attgccacac caaaattttt caatcggcat gttgcagcaa actcgtcctg
    73801 gcgatttaca aacaggcacg gctcaccttc acactccagc accgaacatg gaacttcgat
    73861 gtcgcttaac gcctggctta gtttatacat caccgaccat gcattatcac catccgggct
    73921 aagcaattta agagccacca ggctgttgtc accgcacggt ccattttgcg gaatcggttc
    73981 aaccttcgaa cctgcgaaac cagcagacca gcgatagttc tgcggcagtc gatggacgat
    74041 tgagagttgt aatgaagtca cttattttcc ccggaagcac attcacttca caatttgttt
    74101 acatcaattt taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc
    74161 tcgcctgcgt ggcctctatg gctctgattt aagtaatatc tgcgggcgtg gtcacgtttg
    74221 cggggctata tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga
    74281 ctttttacat aaattgattt tacataaaat aaacatatat cgggggaatg atagatttgt
    74341 ggttgacatg catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt
    74401 tttttcggtc gactggcgta cagacagaag agaatggagc tgcttgcgca gaatgcaatt
    74461 gaccaaatca tcggccatgc agagttaaag gtcgcaagag aaagcaatgc gccaacaatt
    74521 gcccctatgc caaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga
    74581 aactcatcaa gaatgaccgc catcgcattg gatgacacca tcgacacgca gcccacaaac
    74641 gccgcaacgc caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc
    74701 cacgctgcca taataaattg tatccacaac cccgagcgga acatatttaa cgcgccaatg
    74761 cggcgaacga agcggctgtt aaaaatggtc atcacgaaca ggaaaacaat gtttagtgca
    74821 aagtaataac caaagttttc cggcgcgacg tggttaattt caatgtagac aaacggtccg
    74881 gcgcttaaga acgagaacat cccggcaaag ctgaaaccac tggcaagcat ataactcagg
    74941 acacgcttat gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc
    75001 tggcgacgct ccggtggtaa ggtttcttta atcaggaaga aaatcattgc cgaagccaga
    75061 atcgccgcta atgccaggat ccagaagatg taatgccagc tcagccacac cagcacccaa
    75121 ccgccgacaa tcggtgccat cagcggtgca atagtggtca ccagcatgac aaacgacatc
    75181 atccgcgaga actcttcttt cgggtaaata tcgcgcatca gggcgttaat gaccacgctg
    75241 gccgcagccg ccgccagccc gtggaagaaa cgcatcacaa tcagctgatc gatggtgttt
    75301 gccaacgcac acgccaccgc ggcggcggca aacaccagcg taccgccgag caccaccggc
    75361 ttacgcccga agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga
    75421 atataagtac tgagggtcat ctgcgtactg cccgcaggta cgccaaactg cgctgaaatt
    75481 accggtagcg cgggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca
    75541 aggataaaaa caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag
    75601 tcatggaaaa gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg
    75661 ggggctaaat cagcatccag cgtaataccg ccaatacgtt cacgatgcag ctcaaccacg
    75721 tggttaccca cggcggcgaa catgcgtttc acctgatgat aacgcccttc gctgatggtc
    75781 agacgaacct gcgttggggt aatcacttcc agcaccgcag gcttagtgag atctttttcg
    75841 ttatgcagct gcacgccttt agcaaattgc tctgccgtat cgtcagctac aggtgattcc
    75901 agtgtcacca gataggtctt ctcgcaatga tggcgcggag aagtaatgcg gtgcgaccac
    75961 tgaccatcat cagtcatcag caccagaccg gtggtatcga tatccagacg tcctgcggcg
    76021 tgcagtttcc aggctaccgg ttcatccaga aaatagagca ccgttgggtg atcagggtcg
    76081 tccgtggagc aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accatgttgc
    76141 tgcgcgagcg ggttgccatc gtaagcgaca tcatgttcag gaagcagttt gaacgctgca
    76201 ttacgaacaa tttcgccatc gacggtgaca cgattgccgc ggatttcacg cccggcaata
    76261 gcacggctaa cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg
    76321 cctgtaaaaa cggaggtcgg gcattgaccc gaaaatctga actgttgtct gcccagtata
    76381 gcggtctaat aacatccctc aagggaaaaa gatccatggc atactattag cagaataatc
    76441 tacctacgcg agaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    76501 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    76561 ggtaaaagcc tggtgattgc cgaactggca cggctggcgc gtggtcgtgt tctggtgctg
    76621 gcacacgtca aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    76681 gccgatattt ttgccgctgg gttaaagcgc aaagagagcc acggtaaagt ggtatttggc
    76741 agcgtgcagt cggtcgctcg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    76801 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    76861 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    76921 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    76981 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    77041 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    77101 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    77161 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    77221 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    77281 gatgctgcac tgattactgg cgacaccccc ggcgctgagc gcgatgtgtt aattgaagat
    77341 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    77401 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    77461 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    77521 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    77581 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    77641 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    77701 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    77761 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    77821 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    77881 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    77941 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    78001 cgaactgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    78061 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    78121 gtcgtcgccc gcatgaaagg tcagtactgg caggtgcgtg aaaaagtgtt cgattacgaa
    78181 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    78241 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    78301 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    78361 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    78421 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    78481 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    78541 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    78601 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    78661 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    78721 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    78781 tccggcgctg caattggtcg cgcaaattcg gcggcatgcc tttaatggtc aaggtatcgg
    78841 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    78901 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    78961 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    79021 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    79081 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    79141 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    79201 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    79261 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    79321 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    79381 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    79441 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    79501 cgtcacgcag gcgcccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    79561 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    79621 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    79681 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    79741 gcagggcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    79801 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    79861 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    79921 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtgatg
    79981 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    80041 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    80101 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    80161 acgaagacaa agcgcactaa gggaaacaga taactggtta tggtaactca tcgtcagcgc
    80221 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    80281 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    80341 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    80401 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    80461 tttttgtccg tcattctggc aacggcggga atgacgctat tactgatcga tagcgaagtc
    80521 tttactcgtt tccatctcca tcttaatccc atcgtctggc aactggttat caacccagac
    80581 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    80641 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    80701 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    80761 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    80821 ctttcgtacc cgatgacggc gcgacgtttt cttgagaagc atggtctgct tgatgcgcag
    80881 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    80941 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    81001 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    81061 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    81121 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    81181 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcgtcagat
    81241 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    81301 cgcacccaat ccgacgagca gaccgccacg cagtggatca actggctggg ccgctacgca
    81361 caagaagata accgctggtt ctcgtgggtc tctttcaatg gcactaacat tgacgacagc
    81421 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    81481 aaccgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt tgtgattatc
    81541 actgccggtc ggggtattcc gctgagcgaa gacgaagaaa cctttgactg gtcccacggt
    81601 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgcg
    81661 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca
    81721 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    81781 gttactgcag cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    81841 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    81901 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    81961 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    82021 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    82081 gtcggaggtt caaatcctct cgtgccgacc aaatcgccca ccggatacca ccggaaatca
    82141 ccgaataaga cagggatagc ccgccaatta cttgattggc gggttttttt atggttttta
    82201 gtcaccgaac atcaccggat ttataagcta tcagccggat tttttagtta tacgtttagt
    82261 tatacgggct gacgtataac taaagccgta taactaaaac cctgtttttt cgtttggaat
    82321 cataagcata tggcgagaaa aacaccccca ttaaccacag tacagatcaa agcagccaga
    82381 ccagcagaaa aggaatacac cctacaggac ggcggagggc tttttctcct gatcaaaccg
    82441 tccggatcaa aactctggag attttcctac taccgaccat cggacaaaaa aagaatattg
    82501 ctgagttttg gatcgcttga tgatgtttcc ctggctgatg ccagaaaacg ccgtagcgag
    82561 tacagggcgt taattcgtgc cggaactgac ccgcaggacc acgagaggca aaaaagagag
    82621 acagaggccc gaagacaagg gaacacgttc gaaaatgtgg caacagcatg gtaccaggtg
    82681 aaaatcagcc agaatctggc ccccaatacg attaaagaca tctggcgatc gctggaaaaa
    82741 tatgtattcc cgttcatcgg caacacgcca atagacaccc tgaccgctcg caggtttgtt
    82801 gaggtgctta cccccatcaa ggaacgtggc aacctggaaa cgctcaagcg agttttacag
    82861 cgtgtaaatg aggtaatgga ttacgccgcc aatagtggga tgattgatgc caatccggct
    82921 atgaatgtgc gtaaggcgtt cccctcccct gtaaaaaaac atatgccaac aatccgcccc
    82981 gaacagctgc cggagcttat gcaggcttta tcagtatcgg caacagaacg gcagaccaga
    83041 ttactgattg aatggcagtt actgaccgta acccgtcccg ttgaagcatc gtcggcacgc
    83101 tgggaagaaa tcgacatgga ggcgcaacgc tggaccatac ccgccggacg catgaagatg
    83161 cgccgcgacc acgttatccc gctttgtgac caggctatgg cagtgctgga ggctatgaaa
    83221 ccaatcagcc accaccgcga ttatgttttc ccaagtctga aagacccacg gcagccgatg
    83281 aacagccaga cagcaaacgc agcattacga cgcatggggt tcgctggcgt gctggtgtct
    83341 catggattac gcgccatatt cagcacagca gcgaacgagg aaggattcga gccggacgta
    83401 atcgaggcgg cacttgccca cgtcgacacc aacgaagtca gacgggcata caaccggagc
    83461 aactacatag aaaaacgcgt ggtgcttatg cgctggtggg gcgaatttgt cgaggctgcg
    83521 gcgacgggcg taaccctcgc cagtggtaaa aggggtatcc gagccgttta gctgtacaga
    83581 aaaccagtaa aaactacgaa aaccatttaa aaccgtcgta taattgcatc acatttaacg
    83641 acgaggcctt gaaaaatgaa accgttaaga tgcaaacaaa tttcagatgc aattgcgacg
    83701 ggctgcaact ggccctgatg gttcagcatg aattttggtc aacctacgat ccggaggaca
    83761 gaacgacggc cccatcaaaa aaacaggtag tagatttcct ggtatcccgt ggagcttcca
    83821 gaaatctggc tgtaagtatt gataaggttg tacgtccggc atccatgaag accggaggca
    83881 ggcccaaaaa atggcggtaa caatcctgaa agcggcagaa atgccgcttt ttttataaat
    83941 ccctttcaaa tcatcaatat aaaaaacggt gtataccgtt taaaaacggt gagtactgtt
    84001 tttccctgca tccgatgatt taccgtattt ctcaccggaa tacaccggat taatgaggta
    84061 aatcacgatg gaagcaatca aaaaagtcat ctttcgccag gaagtaaaaa aacttatcca
    84121 tattaaggca gacagcacgc tgcaaagcat gatcaacgcc ggagaattcc cgcagggttt
    84181 tcgtgtcggt ttgcgccgtc gcggatggta tgaggaagac gtaatcgcat ggatgaaaga
    84241 acgcgaacaa gaagcgcgcg gaacgaccgc ttaacatata tagggcaaat gatgaacata
    84301 aaaaaagcgg ccccgaaatg gagccacctt tctgaacaat taacccgctg cgccgtatgt
    84361 gtcaacaatc ataagcctgg gcatgataac cgcgatcagg ctggtgggca attatcttgc
    84421 ttgttggggc cgtattgtgc tgccattttc atccgttcag aacgtgaata tttttttaga
    84481 aactcatcaa ggacaaaagc gcatggtgcg aatctttctg attcatgctc gagcgccata
    84541 ttttcgtgct ctctgttcgt tttttgcgat ggtgtattgg ttgattcttt attggtcatt
    84601 gtgtgtacct gtaaagcaat gcgccgtagt acctcacgcc acggcgctga tgttttttat
    84661 cctttgggtt caataccgcg ttgctgtaat tcttttcgga gtactcgctt gatccaggtg
    84721 gctaaagaag tgtcaccgtc tgctttcgct gcctcttcaa gttgtgctct gaattcttct
    84781 ggtagccgca tttggtatgg aggcgatcgc ttttccaatg gtgttgacat ggtaattacc
    84841 cttaattact atagttacat ggtaattacc attgtactta ccgttataca aaaaacaacg
    84901 ccccgcagtg gtggctcaca tacggggcgt ctaaccaaac cattaattga ggtaacgatt
    84961 atggctataa aagagcatag cttactcttt atacacacac aaacgccccg gaaacggaat
    85021 aatccgtttc atagctccgt tttgtgtact catcctggcg ggcgattctt acgggacttt
    85081 agccgccgtg ggcagcttgc taaaaatgcc tccaacgcga atagtccagg tttcatttct
    85141 atcggtatta gttggatatt cagaggatta gccagttcgg cttcaaggcg agcacaggtt
    85201 tctaactgcc tttgccgaat gatttcatct tcttgcgtct gcgtgtcttg tattggtgat
    85261 agtgtgttgt tggtcatagt actgccctgt aaagcaatgc gccgtagtac ctcacaccac
    85321 ggcgctgata gtgattattc tgattctttg gccttgcggc gctggagttc ttcacgtgcg
    85381 acagtgacga gctgcccgat ctcctcggcg gctttgattc cgagtttttc cacctgcgcc
    85441 agtgcattga gcgaagaaat caggaggttt tctccgcttc cttctgcctg gcggcgggcg
    85501 atttcaccgc gcatggcggt tactatgaat ccggcgttgc tttcaccgtc cagttttacg
    85561 gattccatgc cttcaataac atcatgcggg atccgagctg tcagtgattg tgattttgcg
    85621 ttttttgaac ctgtagccat ctgtaatcct ctcaatgaaa gtgtaagaca atatacacat
    85681 aaaaagtctt acataaaagc attgacatgt aagccaccta taaataaagt tacttacacc
    85741 ttgttaatgc aaggtgcaga aacgacgaaa ccccgcactg taggagcagt aacggggctt
    85801 ctaaccacca acgataacga gagtatcgag gtagctatga gaaatcatac cacacacccg
    85861 caagggcggg actcgcacaa cctgaataaa tacatttggc gttttatcgc cctgagcact
    85921 gcacaaccgc gcgtgattac cattgaggcc accagcgaac aggaagcacg cctgcaatcc
    85981 ccggctggct gcgtgatggt attcgctgcc cgtattcgtc agggggtagg cttatgagcc
    86041 aggaaatcac actacaacag gcagcagaac gcgcccacca aatcgaagtt atttgcgcac
    86101 tggcagagga ttacccaagc atgatgaccg acagagaatc aggggcaatc atcagcttac
    86161 ttaaacgcct tagcggtgag gtctgcgtat tcctgatcga tgaacaggaa agaagaacgc
    86221 ttatttctaa cgaaaaaaat gcggaggggt acatgtgcaa taaaaccaca ccggacgcag
    86281 ccgccgcgct cactacgctg atacacgcac ttattgatat tgaatgcacg gcagagcttg
    86341 cgcagggaga agaacagaaa gaccggacac agttcgccct ggaatgtatc cgatacatcg
    86401 caacgcggtc gctgaatgac gctaaaaata ttcttgttgc tgattgtgaa aatggggggg
    86461 ggttatgcgt gatgatcgtt ttaattccct gaaacaagaa ttttccggcg ttcctgatga
    86521 tgcggctgat gcgctttcgt caatgccaga acttattaga gcggcttttt tcttactttc
    86581 cacgagagaa tataaatcaa cgggacttga tgtactgaat atcgccgccg attatgcgga
    86641 atatgtggca gaggcgcgtt acagaagaaa atttcctgag gatgtaagcc atgcgtgata
    86701 tttatcttga aacaatagac cgcgcatcac ttgcactttc tcacagtgaa aacatgatgg
    86761 aaatattgcg gatgtgcctt gaatcctttg gcgataatga acgtaatgca aaaaaaacgc
    86821 ggattattac atcattaata acgcttcttg agtctgtaat aaatgagttg caggaaatag
    86881 agacattgca cgacagatat aacgaacagc acaccggaga ataaaaataa tgaaacttaa
    86941 atattctggc ttaactgcca gtggcaacac tcaccctaaa tttacgcgcg gtgatattta
    87001 ccgcgaccag tacggcggca cggtaatgat taagggcgtg gcgggacggt gcgtaactta
    87061 ccgccgtgaa ggttacgaat atgattgcgt gatgcctgtt tatcagttcc ggcgtgattt
    87121 ttctctggta cagaccgcgc cgcataacgt gcccaccagc aacgccaggg cacgggcaaa
    87181 catccagaag ctgaaaacca tgattaacgg attcaggggc aagaaatgaa aagcgcaccg
    87241 aacttaaaaa aacagcctta cgacaagatg accgaagtca tcatttttgc gggtagtgat
    87301 gcgtgggcac atgcgaaaca gtggcaggaa caggacgggc gactggctgg cgataatgtg
    87361 cctcccgttg tgctggctga tgatcaactg gatgaactgg cagacctgag aatcatcgac
    87421 gaggggcgct attgtgtccg gctgtacaag gcaggccaca tcaggccatc aaatattaat
    87481 gccattgcgc acaagctggc ggcggcgggt gtaactgatg cgaattatta ccccgaaggg
    87541 atgcacagcc agaagcggga gaactggcgc gaatatctgg aacgggagcg ggggaaaaag
    87601 ccaggggaag aaaaacacca ccagcgaaaa accacgctac caatgagcgt gggatctgcc
    87661 ggatacgaca cacaacttga ttacgtggtt aaggggatta ttccggcggt atcgctgtgc
    87721 agcatatacg gggcgagcgg ttcatataaa tcgttccttg cgggttcgtg ggcgtgtcac
    87781 gtttccacgg gccgccagtg gggaggccgc agggtggcgc atggcgctgt tctctatgtg
    87841 gttggtgaag gcggtatcgg cgttccgcgt cgtgtaaaag cctgggaggt tgtgcacggt
    87901 gagcaggtga aaaatctgta tctggtaaac cgtcccatct ttccggctgc cccgcttgat
    87961 gttgaggaaa tggttatcgc tgcccgtcag gtggaacggg aaacgggtaa accggttcga
    88021 atgattattc tggatacgct ggcgcgctgc tttggcggca atgatgaaaa tgattcccgt
    88081 gatatggggg cgtttatccg tgggtgtgac gaactgaaac ggcgcacagg ggcaacggtg
    88141 ctggtggttc accattccgg caaggatgaa acaaaggggg cgcgtggttc cagtgcattt
    88201 cgtgcatcac tggacgccga ataccggata cgcagggagg acgcaggcag cgaagcgctg
    88261 gttatctcat gtaccaaaat gaaggacgcg gaggaactca aagaagcagc atatgactta
    88321 cgcgtggtgg agctttttac cgacgctgac ggggagttaa tcacgtcgct ggtggtggtg
    88381 gataagccgc gccctcccgt tgaactggag cgcatcgagg aggcagggaa caagacggag
    88441 aatcacgccg cgctatggca gtgcatcaga acacgaacag cacacgggga ggcgtgcact
    88501 atcgcccttc tccgcgacga tatgaaaaag ctggggtatg agatgaagca cttccggcgc
    88561 tggctgtaca agctggaaaa tgatggcgta atcgttattg acggtgatga cgtgcgcccg
    88621 ctgtaaaaag tggggagtaa aagtgggggg agtggggaat ttaacaaaat tgaaacataa
    88681 ttacccactt tcccacctgt atatacccaa aaaagtgggg agtaaaaaac atattgaaaa
    88741 acataacgtt agaatcacaa aaaaaagagg tggggagacg gtgggtaatt tcaaaaagtg
    88801 gggagcaaaa gtggggagta gtggggaatg aacagaaaaa ccagagataa gacagcgcca
    88861 aaatataaag cgttagacat gacagagctt tccttaaagg tggcaatcag aacgatagac
    88921 cgccacgcgg gggaaggata cgcgaaggag catcccgaac tgataagcgc attcatgacc
    88981 acagcggcgg caaactttgc cacgctgaca gaacgggaga ttgccgaagc ggaacaggta
    89041 acaaccatca acgttaaaag cgctggagaa taaccgaaga agtgagcaca tagccggagc
    89101 aatccggctt tttttcgatc tcatatatct tcaaagatgg ttaattaatg ataatctatc
    89161 aaaaatactg gttatgcaat gataagtatc aggagaaaac aaagtggaag agataaagca
    89221 aagcgctacc gtgatgattg atcatgaaat ccttaacgag cttaaaaagt tgcagttcga
    89281 tattgtgcgc cggatgaatg aatcaggtat ctacggcagg gcggcaacac caacaatagg
    89341 gcatcttgcg cgcgtactgt tacgcgaacg cctgggcatt gcaacaagtg aggcgtaaac
    89401 aatggctatc acagaacgcg aggcaagcat cattaaagcc attggcgagg aaacaagaga
    89461 caccatagcg ccattgatgg aaaagatcgc agagcttgag acacgaataa acaagctgag
    89521 tgatgaagtt gaaggcctga tctcgttact cggtgagcaa tgacaggggg ggagtgttcc
    89581 ggccggaaag tcgatcgccc tggacaccgc cccccgtctc attcatagaa aattttgctt
    89641 ttcaaaaaag ttacacgaaa agttacaccc cctcaaaacg aggtttttgc atggcattaa
    89701 cgaccaaaaa aaagcggttt gctgatgcgt tatcaaaggg cacaaatcca acggaagccg
    89761 cgatacatgc gggatacagc gaaaagactg caagggttaa aggctggcag ctatccaatg
    89821 atccggaggt gaaaaagtat cttaaggctg taacttctga tgtaactttt caggttacaa
    89881 aaccgaaaaa agttacaaag aaagttaccg aaaaagttac accggaacgg gcatcaatta
    89941 acaccattga gatgatggat aacggcctac ccgaccccat caaagcaatg ggcaggatac
    90001 tgattgacaa catggacaca gatcaaaaac tggctttaga tgccgcgttt aagctggccc
    90061 agttcaccca ccgcaagatc ggcagcattg ggaaaaaaga ggttaaaaaa ctcaatgccg
    90121 ctaatgtctc ctcaaatcgg ttccccatcc ctaagccacc aactggctta aaacactgag
    90181 gtagcgaact atgagcgaat acatcacaga ccctattgat gtactcacgg aaatcatgaa
    90241 cgacaagcag cagccaaccg aaatacggtt acaggctgcc gctgttctga tgccatattt
    90301 tcacgaaacc ctaaccccat gttaccagga ggaagaagat gatgatttct aaaaacatga
    90361 aaggcgtgtc ggcagttcgc gccgccagcg tgcttacatc gtgtaaattc agccatgcag
    90421 acgcaattga aatgacgaaa aaaaatgatc cggcagtatc gaaatttctt gaaaaatcca
    90481 ttgttgccgg aatgactacg gataatatgc cgtcgctggt ggcagagccg atagtaagag
    90541 agcttggcga gtatattttt tcgcagtctg taccaggcaa gctaatcagc agcgcgctga
    90601 atcttccgtt tggcgcaaaa ttgcagtcaa tatcaggcaa gggtagtaaa tggcttaaag
    90661 aagccgaaga aatccctgtc aaagagggag aagtaaacga aagtgcgcta aaactgtgca
    90721 aacttggcgc tattgtgatg ctgaataacg agctaatcaa atttagcacc ccttgtacca
    90781 attcagcgat cagagacttg atcacatcgg aatcggtgaa agaaatagat aaaaaatttc
    90841 tttccagtga ccaggaggtg gcgggcgtat cccctgaggg cgtgcttaat ggtgctgatg
    90901 aggccgaaaa cttcatagag cttttcaaaa agcacatagc gaacggtaac acgcttgcca
    90961 cgtcgtcgct aatcttgccc gtagaaaacg ccttacagct tactgatgct gagttctggc
    91021 agatagagct tttgagaata ccggtaattg tgagcgaaca ggcggatcgc atgatgctga
    91081 tagacgctca aaagctgatt attaacgttg aagccacagt tataacccca actgatgagg
    91141 cggtaataaa aaccgaatca gggacggtta gccttttcca gaatgacgct acagcgttca
    91201 gggcaatcac ctattgcgga tggcaaaaac tcgataaggc cgtcaccgtt ctggcgaaaa
    91261 gctgattttt ccggcatcaa tcccgatatg gtgatcccca tatcgggata attacgaaat
    91321 tttcgcagtt taattaatgg tcagattaat cgctaacccg ctgatatttt cggaaacctc
    91381 aatttgagga agtcggaagc gtcacccttc aaatcctgaa tggtcataat gaccacgcag
    91441 accatcaagg caaccataac gaacatcgtt acggttggag cttttaccac aggaaaactc
    91501 gcatttaaat tgtacacttt ttcagcaaat g
//
LOCUS       DAZUFB010000006        87014 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZUFB010000006 DAZUFB010000000
VERSION     DAZUFB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.006"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..301)
                     /locus_tag="ACHY3M_000480"
     CDS             complement(<1..301)
                     /locus_tag="ACHY3M_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4315458.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMD"
     gene            complement(372..2486)
                     /gene="fusA"
                     /locus_tag="ACHY3M_000481"
     CDS             complement(372..2486)
                     /gene="fusA"
                     /locus_tag="ACHY3M_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4315459.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACHY3M_000482"
     CDS             complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACHY3M_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4315460.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3150..3524)
                     /gene="rpsL"
                     /locus_tag="ACHY3M_000483"
     CDS             complement(3150..3524)
                     /gene="rpsL"
                     /locus_tag="ACHY3M_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4315461.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3650..3937)
                     /gene="tusB"
                     /locus_tag="ACHY3M_000484"
     CDS             complement(3650..3937)
                     /gene="tusB"
                     /locus_tag="ACHY3M_000484"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFS4315462.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3945..4304)
                     /gene="tusC"
                     /locus_tag="ACHY3M_000485"
     CDS             complement(3945..4304)
                     /gene="tusC"
                     /locus_tag="ACHY3M_000485"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFS4315463.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4304..4690)
                     /gene="tusD"
                     /locus_tag="ACHY3M_000486"
     CDS             complement(4304..4690)
                     /gene="tusD"
                     /locus_tag="ACHY3M_000486"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFS4315464.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4690..5412)
                     /gene="yheO"
                     /locus_tag="ACHY3M_000487"
     CDS             complement(4690..5412)
                     /gene="yheO"
                     /locus_tag="ACHY3M_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4315465.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5579..6391)
                     /gene="fkpA"
                     /locus_tag="ACHY3M_000488"
     CDS             complement(5579..6391)
                     /gene="fkpA"
                     /locus_tag="ACHY3M_000488"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4315466.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6612..6830
                     /gene="slyX"
                     /locus_tag="ACHY3M_000489"
     CDS             6612..6830
                     /gene="slyX"
                     /locus_tag="ACHY3M_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFS4315467.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6879..7469)
                     /gene="slyD"
                     /locus_tag="ACHY3M_000490"
     CDS             complement(6879..7469)
                     /gene="slyD"
                     /locus_tag="ACHY3M_000490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4315468.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7564..7764)
                     /gene="yheV"
                     /locus_tag="ACHY3M_000491"
     CDS             complement(7564..7764)
                     /gene="yheV"
                     /locus_tag="ACHY3M_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFS4315469.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIIECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7774..9578)
                     /gene="kefB"
                     /locus_tag="ACHY3M_000492"
                     /pseudo
     CDS             complement(7774..9578)
                     /gene="kefB"
                     /locus_tag="ACHY3M_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9578..10098)
                     /gene="kefG"
                     /locus_tag="ACHY3M_000493"
                     /pseudo
     CDS             complement(9578..10098)
                     /gene="kefG"
                     /locus_tag="ACHY3M_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
     gene            10224..12137
                     /gene="yheS"
                     /locus_tag="ACHY3M_000494"
     CDS             10224..12137
                     /gene="yheS"
                     /locus_tag="ACHY3M_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4315470.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12137..13159
                     /gene="yheT"
                     /locus_tag="ACHY3M_000495"
     CDS             12137..13159
                     /gene="yheT"
                     /locus_tag="ACHY3M_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4315471.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGSSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13153..13371
                     /gene="yheU"
                     /locus_tag="ACHY3M_000496"
     CDS             13153..13371
                     /gene="yheU"
                     /locus_tag="ACHY3M_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFS4315472.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13425..14294
                     /gene="prkB"
                     /locus_tag="ACHY3M_000497"
     CDS             13425..14294
                     /gene="prkB"
                     /locus_tag="ACHY3M_000497"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFS4315473.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14349..14753)
                     /gene="yhfA"
                     /locus_tag="ACHY3M_000498"
     CDS             complement(14349..14753)
                     /gene="yhfA"
                     /locus_tag="ACHY3M_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFS4315474.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAV"
     gene            15055..15687
                     /gene="crp"
                     /locus_tag="ACHY3M_000499"
     CDS             15055..15687
                     /gene="crp"
                     /locus_tag="ACHY3M_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFS4315475.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15738..17828
                     /gene="yhfK"
                     /locus_tag="ACHY3M_000500"
     CDS             15738..17828
                     /gene="yhfK"
                     /locus_tag="ACHY3M_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFS4315476.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17895..19115)
                     /gene="argD"
                     /locus_tag="ACHY3M_000501"
                     /gene_synonym="dapC"
     CDS             complement(17895..19115)
                     /gene="argD"
                     /locus_tag="ACHY3M_000501"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFS4315477.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19201..19764)
                     /gene="pabA"
                     /locus_tag="ACHY3M_000502"
     CDS             complement(19201..19764)
                     /gene="pabA"
                     /locus_tag="ACHY3M_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFS4315478.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19796..20398)
                     /gene="fic"
                     /locus_tag="ACHY3M_000503"
     CDS             complement(19796..20398)
                     /gene="fic"
                     /locus_tag="ACHY3M_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFS4315479.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20388..20555)
                     /gene="yhfG"
                     /locus_tag="ACHY3M_000504"
     CDS             complement(20388..20555)
                     /gene="yhfG"
                     /locus_tag="ACHY3M_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFS4315480.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20660..21232)
                     /gene="ppiA"
                     /locus_tag="ACHY3M_000505"
                     /gene_synonym="rotA"
     CDS             complement(20660..21232)
                     /gene="ppiA"
                     /locus_tag="ACHY3M_000505"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFS4315481.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21503..22684
                     /gene="tsgA"
                     /locus_tag="ACHY3M_000506"
     CDS             21503..22684
                     /gene="tsgA"
                     /locus_tag="ACHY3M_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFS4315482.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22946..25489
                     /gene="nirB"
                     /locus_tag="ACHY3M_000507"
     CDS             22946..25489
                     /gene="nirB"
                     /locus_tag="ACHY3M_000507"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFS4315483.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25486..25812
                     /gene="nirD"
                     /locus_tag="ACHY3M_000508"
     CDS             25486..25812
                     /gene="nirD"
                     /locus_tag="ACHY3M_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFS4315484.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25938..26744
                     /gene="nirC"
                     /locus_tag="ACHY3M_000509"
     CDS             25938..26744
                     /gene="nirC"
                     /locus_tag="ACHY3M_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFS4315485.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26763..28136
                     /gene="cysG"
                     /locus_tag="ACHY3M_000510"
     CDS             26763..28136
                     /gene="cysG"
                     /locus_tag="ACHY3M_000510"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFS4315486.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRTGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQKMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28383..28550
                     /gene="yhfL"
                     /locus_tag="ACHY3M_000511"
     CDS             28383..28550
                     /gene="yhfL"
                     /locus_tag="ACHY3M_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFS4315487.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28845..30182
                     /gene="frlA"
                     /locus_tag="ACHY3M_000512"
     CDS             28845..30182
                     /gene="frlA"
                     /locus_tag="ACHY3M_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFS4315488.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30203..31225
                     /gene="frlB"
                     /locus_tag="ACHY3M_000513"
     CDS             30203..31225
                     /gene="frlB"
                     /locus_tag="ACHY3M_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFS4315489.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31275..32105
                     /gene="frlC"
                     /locus_tag="ACHY3M_000514"
     CDS             31275..32105
                     /gene="frlC"
                     /locus_tag="ACHY3M_000514"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFS4315490.1"
                     /translation="MKTGMFTCGHQRLPIEHVFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32102..32887
                     /gene="frlD"
                     /locus_tag="ACHY3M_000515"
     CDS             32102..32887
                     /gene="frlD"
                     /locus_tag="ACHY3M_000515"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4315491.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNMAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32987..33718
                     /gene="frlR"
                     /locus_tag="ACHY3M_000516"
                     /gene_synonym="yhfR"
     CDS             32987..33718
                     /gene="frlR"
                     /locus_tag="ACHY3M_000516"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFS4315492.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33870..34955)
                     /gene="yhfS"
                     /locus_tag="ACHY3M_000517"
     CDS             complement(33870..34955)
                     /gene="yhfS"
                     /locus_tag="ACHY3M_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4315493.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34967..36271)
                     /gene="yhfT"
                     /locus_tag="ACHY3M_000518"
     CDS             complement(34967..36271)
                     /gene="yhfT"
                     /locus_tag="ACHY3M_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFS4315494.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36283..36636)
                     /gene="yhfU"
                     /locus_tag="ACHY3M_000519"
     CDS             complement(36283..36636)
                     /gene="yhfU"
                     /locus_tag="ACHY3M_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFS4315495.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAAFSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36647..37525)
                     /gene="php"
                     /locus_tag="ACHY3M_000520"
     CDS             complement(36647..37525)
                     /gene="php"
                     /locus_tag="ACHY3M_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFS4315496.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37522..38748)
                     /gene="yhfW"
                     /locus_tag="ACHY3M_000521"
     CDS             complement(37522..38748)
                     /gene="yhfW"
                     /locus_tag="ACHY3M_000521"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4315497.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38748..39911)
                     /gene="yhfX"
                     /locus_tag="ACHY3M_000522"
     CDS             complement(38748..39911)
                     /gene="yhfX"
                     /locus_tag="ACHY3M_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFS4315498.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39995..40357)
                     /gene="yhfY"
                     /locus_tag="ACHY3M_000523"
     CDS             complement(39995..40357)
                     /gene="yhfY"
                     /locus_tag="ACHY3M_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4315499.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40374..41279)
                     /gene="yhfZ"
                     /locus_tag="ACHY3M_000524"
     CDS             complement(40374..41279)
                     /gene="yhfZ"
                     /locus_tag="ACHY3M_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFS4315500.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41549..42553)
                     /gene="trpS"
                     /locus_tag="ACHY3M_000525"
     CDS             complement(41549..42553)
                     /gene="trpS"
                     /locus_tag="ACHY3M_000525"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4315501.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42546..43304)
                     /gene="gph"
                     /locus_tag="ACHY3M_000526"
     CDS             complement(42546..43304)
                     /gene="gph"
                     /locus_tag="ACHY3M_000526"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFS4315502.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43297..43974)
                     /gene="rpe"
                     /locus_tag="ACHY3M_000527"
     CDS             complement(43297..43974)
                     /gene="rpe"
                     /locus_tag="ACHY3M_000527"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4315503.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43992..44828)
                     /gene="dam"
                     /locus_tag="ACHY3M_000528"
     CDS             complement(43992..44828)
                     /gene="dam"
                     /locus_tag="ACHY3M_000528"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS4315504.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44935..46221)
                     /gene="damX"
                     /locus_tag="ACHY3M_000529"
     CDS             complement(44935..46221)
                     /gene="damX"
                     /locus_tag="ACHY3M_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFS4315505.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46313..47401)
                     /gene="aroB"
                     /locus_tag="ACHY3M_000530"
     CDS             complement(46313..47401)
                     /gene="aroB"
                     /locus_tag="ACHY3M_000530"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4315506.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47458..47979)
                     /gene="aroK"
                     /locus_tag="ACHY3M_000531"
     CDS             complement(47458..47979)
                     /gene="aroK"
                     /locus_tag="ACHY3M_000531"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFS4315507.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48380..49618)
                     /gene="hofQ"
                     /locus_tag="ACHY3M_000532"
     CDS             complement(48380..49618)
                     /gene="hofQ"
                     /locus_tag="ACHY3M_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFS4315508.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49530..49934)
                     /gene="hofP"
                     /locus_tag="ACHY3M_000533"
     CDS             complement(49530..49934)
                     /gene="hofP"
                     /locus_tag="ACHY3M_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFS4315509.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49924..50364)
                     /gene="hofO"
                     /locus_tag="ACHY3M_000534"
     CDS             complement(49924..50364)
                     /gene="hofO"
                     /locus_tag="ACHY3M_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFS4315510.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50348..50887)
                     /gene="hofN"
                     /locus_tag="ACHY3M_000535"
     CDS             complement(50348..50887)
                     /gene="hofN"
                     /locus_tag="ACHY3M_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFS4315511.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50887..51666)
                     /gene="hofM"
                     /locus_tag="ACHY3M_000536"
     CDS             complement(50887..51666)
                     /gene="hofM"
                     /locus_tag="ACHY3M_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFS4315512.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51786..54338
                     /gene="mrcA"
                     /locus_tag="ACHY3M_000537"
     CDS             51786..54338
                     /gene="mrcA"
                     /locus_tag="ACHY3M_000537"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFS4315513.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54503..55063)
                     /gene="nudE"
                     /locus_tag="ACHY3M_000538"
     CDS             complement(54503..55063)
                     /gene="nudE"
                     /locus_tag="ACHY3M_000538"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFS4315514.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55383..57518
                     /gene="igaA"
                     /locus_tag="ACHY3M_000539"
     CDS             55383..57518
                     /gene="igaA"
                     /locus_tag="ACHY3M_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFS4315515.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57583..58251
                     /gene="yrfG"
                     /locus_tag="ACHY3M_000540"
     CDS             57583..58251
                     /gene="yrfG"
                     /locus_tag="ACHY3M_000540"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFS4315516.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58262..58663
                     /gene="hslR"
                     /locus_tag="ACHY3M_000541"
     CDS             58262..58663
                     /gene="hslR"
                     /locus_tag="ACHY3M_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFS4315517.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58688..59566
                     /gene="hslO"
                     /locus_tag="ACHY3M_000542"
     CDS             58688..59566
                     /gene="hslO"
                     /locus_tag="ACHY3M_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4315518.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59701..61425)
                     /gene="yhgE"
                     /locus_tag="ACHY3M_000543"
     CDS             complement(59701..61425)
                     /gene="yhgE"
                     /locus_tag="ACHY3M_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFS4315519.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61803..63425
                     /gene="pckA"
                     /locus_tag="ACHY3M_000544"
     CDS             61803..63425
                     /gene="pckA"
                     /locus_tag="ACHY3M_000544"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4315520.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63501..64853)
                     /gene="envZ"
                     /locus_tag="ACHY3M_000545"
     CDS             complement(63501..64853)
                     /gene="envZ"
                     /locus_tag="ACHY3M_000545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFS4315521.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64850..65569)
                     /gene="ompR"
                     /locus_tag="ACHY3M_000546"
     CDS             complement(64850..65569)
                     /gene="ompR"
                     /locus_tag="ACHY3M_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFS4315522.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65797..66273
                     /gene="greB"
                     /locus_tag="ACHY3M_000547"
     CDS             65797..66273
                     /gene="greB"
                     /locus_tag="ACHY3M_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFS4315523.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66370..68691
                     /gene="yhgF"
                     /locus_tag="ACHY3M_000548"
     CDS             66370..68691
                     /gene="yhgF"
                     /locus_tag="ACHY3M_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4315524.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69129..69356
                     /gene="feoA"
                     /locus_tag="ACHY3M_000549"
     CDS             69129..69356
                     /gene="feoA"
                     /locus_tag="ACHY3M_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFS4315525.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69373..71694
                     /gene="feoB"
                     /locus_tag="ACHY3M_000550"
     CDS             69373..71694
                     /gene="feoB"
                     /locus_tag="ACHY3M_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFS4315526.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71694..71930
                     /gene="feoC"
                     /locus_tag="ACHY3M_000551"
     CDS             71694..71930
                     /gene="feoC"
                     /locus_tag="ACHY3M_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFS4315527.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72133..73011
                     /gene="rpnA"
                     /locus_tag="ACHY3M_000552"
     CDS             72133..73011
                     /gene="rpnA"
                     /locus_tag="ACHY3M_000552"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFS4315528.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73040..73810)
                     /gene="bioH"
                     /locus_tag="ACHY3M_000553"
     CDS             complement(73040..73810)
                     /gene="bioH"
                     /locus_tag="ACHY3M_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFS4315529.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73848..74531
                     /gene="gntX"
                     /locus_tag="ACHY3M_000554"
     CDS             73848..74531
                     /gene="gntX"
                     /locus_tag="ACHY3M_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFS4315530.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74590..75165
                     /gene="nfuA"
                     /locus_tag="ACHY3M_000555"
     CDS             74590..75165
                     /gene="nfuA"
                     /locus_tag="ACHY3M_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFS4315531.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75526..76842
                     /gene="gntT"
                     /locus_tag="ACHY3M_000556"
     CDS             75526..76842
                     /gene="gntT"
                     /locus_tag="ACHY3M_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS4315532.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76887..78971)
                     /gene="malQ"
                     /locus_tag="ACHY3M_000557"
     CDS             complement(76887..78971)
                     /gene="malQ"
                     /locus_tag="ACHY3M_000557"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFS4315533.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(78981..81374)
                     /gene="malP"
                     /locus_tag="ACHY3M_000558"
     CDS             complement(78981..81374)
                     /gene="malP"
                     /locus_tag="ACHY3M_000558"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFS4315534.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            81986..84691
                     /gene="malT"
                     /locus_tag="ACHY3M_000559"
     CDS             81986..84691
                     /gene="malT"
                     /locus_tag="ACHY3M_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFS4315535.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84734..85750)
                     /gene="rtcA"
                     /locus_tag="ACHY3M_000560"
     CDS             complement(84734..85750)
                     /gene="rtcA"
                     /locus_tag="ACHY3M_000560"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFS4315536.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85754..86980)
                     /gene="rtcB"
                     /locus_tag="ACHY3M_000561"
     CDS             complement(85754..86980)
                     /gene="rtcB"
                     /locus_tag="ACHY3M_000561"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFS4315537.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20751 a        23777 c        21981 g        20505 t
ORIGIN      
        1 cgtccatctg agcagcaccg gtgatcatgt ttttaacata gtcggcgtgc cccgggcagt
       61 ctacgtgtgc gtagtgacgg gtcggggtgt catattcaac gtgagaagtg ttgatggtga
      121 taccacgagc tttttcttcc ggcgcgttat cgatctggtc gaatgcacga gcagcaccgc
      181 cgtaggtttt agccagtacg gtggtgattg cagcggtcag agtagtttta ccgtggtcaa
      241 cgtggccgat agtaccaacg ttaacgtgcg gttttgtacg ttcaaatttt tctttagaca
      301 cggctatatt ccttactata gtgctctccc cttcaggaga gagcacggga ctttggtatt
      361 aacccttagg cttatttacc acgggcttca attacggcct gagcaacgtt actcggcgct
      421 tcatcatact tcaggaattc catagtgtat gatgcacgac ctttggtcag agaacgcagc
      481 tgagttgcgt atccgaacat ttcagacagc ggtacttcag cgtggatctt aacgccagta
      541 acttcagatt cctgaccttt gagcatacca cgacgacggc tcaagtcacc gataacgtca
      601 ccggtgttct cttccggagt ttctacttca accttcatga tcggctcaag cagaactggt
      661 ttcgctttct taaagccttc tttaaaggcg atagaagcag ccagtttaaa cgccagttca
      721 gaggagtcaa cgtcatggta agaaccgaag tgcagacgaa tacccatgtc tactaccggg
      781 tagcctgcca gcggacctgc tttcagctgt tcctggatac ctttatcaac ggccgggatg
      841 tattcgccag ggattacacc acctttaatg tcgttgatga actcgtagcc tttcgggttt
      901 gaacccggct ccagcgggta catgtcgata acaacatgac catactgacc acgaccacca
      961 gactgtttcg cgtgtttacc ttcaacatcg gtaactttct ggcggatagt ttcacggtaa
     1021 gcaacctgcg gtttacctac gttcgcttca acgttgaatt cacgcttcat acggtcaacg
     1081 atgatgtcga ggtgcagttc gcccataccc gcgataatgg tctggttaga ttcttcgtca
     1141 gtccatacac ggaaagacgg gtcttcttta gccagacggc ccagagccag acccattttt
     1201 tcctggtcag ctttggtttt cggttcaact gcgatggaga ttaccggctc agggaattcc
     1261 atacgttcca gaatgatcgg cgcatccggg tcacacaggg tgtcaccagt ggttacgtct
     1321 ttcagaccga tagcagcagc gatgtcgccc gcgcgaactt ctttgatctc ttcacgtttg
     1381 ttagcgtgca tctgaacgat acgaccgaaa cgctcacgtg cagctttcac ggagttcagt
     1441 acggtatcac cagagttaac cacaccggag taaacacgga agaaggtcag gttaccaaca
     1501 aacgggtcgg tagcgatttt gaacgccagt gcagaaaacg gctcgtcatc acttgcgtga
     1561 cgctcagccg gagtgtcttt accgtcgtcc aggataccgt tgatcgcagg tacgtcaacc
     1621 ggggatggca ggtaatcaat taccgcatcc agcatcgcct gaacaccttt gttcttgaac
     1681 gcagaaccac aggttaccag gatgatttcg ttgttcagaa cgcgctgacg cagagcacct
     1741 ttgatttctg cttcagtcag ttcttcacca cccaggtatt tttccatcag ctcttcagaa
     1801 gcttcagctg cggattcgat caggttctgg tgccattcgt tagccagttc aaccatgtct
     1861 gccgggatat cttcgtattc gaaggttacg ccctggtcag cgtcgttcca gttgatagct
     1921 ttcattttca ccaggtcaac aacaccggtg aaatgttctt cagcaccaat cgccagctgc
     1981 agcggaaccg ggttcgcgcc cagacgggtt ttgatctggt taacaacttt caggaagttc
     2041 gcacccatgc ggtccatttt gttaacgaac gcaatgcgcg gaactttata tttgtttgcc
     2101 tgacgccata cggtttcaga ctgcggctga acaccaccaa ctgcgcagta aaccattacc
     2161 gcaccatcga gaacacgcat ggaacgttct acttcgattg tgaagtcaac gtgccccggg
     2221 gtgtcgatga tgttgatgcg atgcggctca tactgcttag ccataccaga ccagaatgca
     2281 gtagtcgcag cggaagtgat ggtaatacca cgttcctgct cctgctccat ccagtccatg
     2341 gttgcagcgc cgtcatgaac ttcaccgatt ttatggttta caccggtgta gaacagaata
     2401 cgttcggtag tagtggtttt accggcgtcg atgtgcgcac tgataccgat attacggtag
     2461 cgtgcgatgg gtgttgtacg agccatttgt ttcctcgttt atcttttagg cgttcaattt
     2521 aagtagccca aagcgggctg cttactggaa gcgcccgcct ggtgactaaa actccgaagg
     2581 gattaccaac ggtagtgtgc gaacgccttg ttggcttcgg ccatacggtg aacgtcttca
     2641 cgtttcttaa ctgcagtacc tttgttttct gcagcatcag aaagttcgtt cgccaggcgc
     2701 agagccatgg atttatcacc gcgtttacga gcagcttcaa cgatccaacg cattgccaga
     2761 gcattacgac gaaccggacg gacttcaact ggtacctgat aagtagaacc accaacgcgg
     2821 cgagacttaa cttctacagt cgggcgcacg ttttcgagag ctacttcgaa tgcttccagt
     2881 tcagatttac cagagcgctg agccagggtc tccagcgcgc tgtatacgat agattcagca
     2941 gtagattttt taccatctac catcaggata tttacaaatt tagccagcag ttctgatccg
     3001 aacttcggat ccggcagaat tttacgctga ccaatgacgc gacgacgtgg catggaaata
     3061 ctccgttgtt aattcaggat tgtccaaaac tctacgagtt tagtttgaca tttaagttaa
     3121 aacgtttggc cttacttaac ggagaaccat taagccttag gacgcttcac gccatacttg
     3181 gaacgagcct gcttacggtc tttaacgccg gagcagtcaa gcgcaccacg tacggtgtgg
     3241 taacgaacac ccgggaggtc tttaacacga ccgccacgga tcaggatcac ggagtgctcc
     3301 tgcaggttgt gaccttcacc accgatgtag gaagtcactt cgaaaccgtt agtcagacga
     3361 acacggcata ctttacgcag cgcggagttc ggttttttag gagtggtagt atatacacga
     3421 gtacatacgc cacgtttttg cgggcatgct tccagcgcag gcacgttgct tttcgcaact
     3481 ttgcgagcac gtggtttgcg taccagctgg ttaactgttg ccattaaata gctcctggtt
     3541 ttagcttttg cttcgtaaac acgtaataaa acgtcctcac acaatatgag gacgccgaat
     3601 tttagggcga tgccgaaaag gtgtcaagaa atatacaacg atcccgccat caccaggcca
     3661 tctggctgga gtgcttaacc gtaagtctga cgaaatcagt atagtcaatg ggaatgatgt
     3721 cgttcgaaat ttgaccagtc aaaccgcggg caataaggtc ttcgttcagg gcatagacct
     3781 taatgggggc attacgcaga ctttcaaggt agcggttacc gtcaactgcg gcagttacgc
     3841 catcttgcaa taatagcagt tcgtctcctt cactgagcag acgcagcagc gcagcaaaat
     3901 ccgtcagcca gggtgaacga tgtaacgtgt gcagcataaa cgcctcaaaa cctcaaaata
     3961 acatcgtagt tggcgagttc gcggcgtaag gcatctgctt cgagcggcgt ggcttcgaca
     4021 acaaagggtg tctgcggatc taacccgcgt tcgcgcagtg aagccgcaca aacccagcac
     4081 tgttcaatgt cgtacagacc caacaattta aaagtggcaa tgtaatcacg cgccagcact
     4141 gcatcgggct tttgtcctga cagcagctga aaaacgccat cagcaataaa gaagacggcc
     4201 agatcgtcag ttaatgcgga agttgccagt aaagcatcta aaccttcccg accagcagct
     4261 gtaccatgag gtgcagtaga aaaaacaaac gcaattcgtt tcatcagaac tgtaccaccc
     4321 tgtcacaggt cagcgaggct tccgccagcg cgccaagtcc gcttaaggta aatcccggct
     4381 gaaggtttga cgaagccagc cccagccttc ctgcttcagt ttcatcgacg ataccgcggc
     4441 gtaacgctgc cgctacgcag atattcagcg ccacaccatg ttgcgcattc aactgttgcc
     4501 agccgcgcac aaggtcaaat tcgtcacttg ccggtgaggt caattggtta gcgttataga
     4561 ccccttcccg atagaaaaag acgctgctta actcatggcc ttctactatc agcgcctgcg
     4621 caaactgaaa agcactactc gcctgttgcg taccgtatgc tggcccggtc accacgatgg
     4681 caaaacgcat tacttatctt gcccctggaa atcaccgctt ttgaactggc ggatgtagag
     4741 atagacagtg tgtttggaga tgttcaggcg gtcagcaacc tggttgatcg cgtctttaat
     4801 atcgaagatc cctttctcgt agagattcag cacgatctga cgatttttgg cgttattaga
     4861 aacattgcga tcggcattca cttcttcgat ggtgaactcc agcgtttggg taaccagatc
     4921 ttcaacagaa gaggcaaagt tgacgcttga accgacatcc ggggtttctg gcggcacaaa
     4981 agtgctcata atctgcgaga agggaacatc aagattcata ttgatgcaca gcagaccaat
     5041 tacacgctgt tcgcggttac gaatcgcgat agtcagggac ttcattaata cgccgctttt
     5101 ggcgcgagta aagtagcatt tagaaacgct gctatccgct cccgtcatat cgtgcagcat
     5161 acgtagcgcc aggtcagtaa ttggcgaacc aatcttccgg cctgtatgtt caccgttagc
     5221 aatgcgaatg gctgaacatt ttagatcctg caaagagtgc aaaacgattt cacagtggga
     5281 gccaataagc atcgctaacc cgtccaccac cgcttcgtag gatttcagaa tatcaaaatc
     5341 ggtctggtcg aaaggacgtt gatccagtaa atccaactca ctggtttcgt tggttaaaag
     5401 cgacctggac atgaaaaaaa acactccttt tcaggagcct gtcgttaact tttcagggca
     5461 ggctcattaa tgatgcgggt aactaaatta atacagcgga ggttccgctt tccagcacta
     5521 attatatccg gcctgtaata aaaaaaccgc cgcctggtca ggcggcggtt cttaatgctt
     5581 attttttagc agaatctgcg gctttcgcat cagcttccgg ctttgcatca gccttcggcg
     5641 ctggtttcac atccagcagc tctacgtcaa acaccagggt agaattcggt gggatccccg
     5701 gaacacccgc tttgccgtaa gccagttctg gtggaataac cagtttgatc ttaccgcctt
     5761 tcttgatgtt cttcagacct tctgtccaac ccgggataac accgtccaga cggaaagaaa
     5821 gcggttcacc acgggtgtaa gagttgtcga actctttacc gtcgatcagc gtacctttgt
     5881 agttcactac aacagtatcg ctgtctttcg gtgcttcgcc tttaccggct tctactacct
     5941 gataaaccag accagttgaa gaggttttca cacctttctc tttggcaaat ttctcgcggt
     6001 actctttacc ttttgcttcg ttatcagccg cgtctttttc catcttcgcc tgagcagaag
     6061 acttcacgcg agcttcgaat gcttgcagag tctgttcgat ctcttggtcg gagagtttgc
     6121 tcttatcagc aaatgcatcc tgaacaccag cgatcagctg atctttatcc agtttgatgc
     6181 ccagtttttc ttgttcttta agagagtttt ccatgtaacg acccagcgaa gcacccagtg
     6241 cataagctga tttctgatcg tcatttttga acgctgcttt gctgtcagca gttgtagcag
     6301 gttttgcagc ttcagcagca aaagtgattg gtgcatgcag ggcaacggcc attgtggtcg
     6361 ccagcagcgt tactttaaac agtgatttca tccatatctc caggatcggg gcatctcacc
     6421 ccagggttaa ctattatcag aagggtacta taaagcgttg tcgaacaaat ctacatacag
     6481 acacgcccta ttatcatcta ttttcagact ctttttgttt aaattagttt cgatgaccgc
     6541 gaaatgagtg ctgtctcggg cagcaaagtt aagtagaatc cgcggcgacc attcgacaaa
     6601 agaggtgaat catgcaggat ttatcattgg aagcacgcct ggcagagctg gagagccgac
     6661 tggcttttca ggaaatcacc attgaagaac tgaacgtcac ggtgaccgct catgaaatgg
     6721 agatggcgaa actgcgcgat catctgcgtc tgctgaccga gaagttaaaa gccagccagc
     6781 cgtcgaacat cgcgtcgcag gctgaagaaa cgccaccgcc acattattga ggcgtaaaaa
     6841 aagcggggat tccccgcttt tttgtcacct tttcggtatc agtggcaacc acaaccgccg
     6901 ttgcctttac cgccacagca gccttcgcca ccgtgttcat gaccgtgatc gtggccatga
     6961 ccgccgcagc aaccgtcgtg gtcgtgatcg tggtgatgat cgtgcgcgcc gtgaacgtgg
     7021 ccatgagcca gttcttcttc ggtcgcttcg cgaatcgcca caacttcaac gttgaatttc
     7081 aggttctgac cggccagcat gtggttacca tcaaccacga cgtgatcgtc ttcaaccgca
     7141 gtgatttcaa ccggtaccgg cccctggtcg gtttcagcca ggaaacgcat acctacctgc
     7201 agttcatcaa cgcccataaa tacgtcttta ggaacacgtt gcaccaggtt ttcgtcgtac
     7261 tgaccgtaag cgtcgttcgc gccaacagcg acatcaaatt tgtcgccaac ttcatgacct
     7321 tccagcgccg tttccaggcc agagatcagg gaaccgtgac catgcaggta gtccagcggc
     7381 gcactcaccg gagactcatc aaccaacaca ccgtcttctg tacgtacctg ataggccagg
     7441 ctgaccacca ggtcttttgc tactttcatg atatctcctg agcatgggaa gaatagtggc
     7501 gcagattgta gcggaattct gcagccgtgt actcactagc ttaaaaaaac tcggcgcata
     7561 tcgctagtcc ggatgaaaaa tcccgatcac ttgctcatct ttgcgaacgt gatcgcgggc
     7621 ttctttgtct gcttctcgca tctgatgtcc gcacttaaca cattcaataa tatcaatatt
     7681 attttcgcgc cacatcgcca ttgaatcctg cgcctgacag gccgggcatt ttgcgcccgc
     7741 gataaaacgt tttcggattg ccatctttac cctctactca aattcatccc agccgtccag
     7801 ctggcgtcgt tcttgttgca tttcacgctg gaaaatctct tccagttcgc gtcgggcttc
     7861 cctggcgcga gaaatttgta cggtatcagc atgcatcggg attaactccc gcagcattcg
     7921 catatccagg cggcgaaaat gcagttgcgc ccgctgtgcc tgatgcagat gcatgccaag
     7981 cgtgaccagc gtcttgcgcc ccagctctaa cgcactggag aatgtttcac gggaaaactg
     8041 cgtcacccct gcctgtaata actcatgcgc ttccacacgt ccgcgcgctc gcgcaagaat
     8101 atgcaaatgc ggaaagtgct gctggcatat ttccaccagc ttcatggtgt cttccggctc
     8161 gttacaggta atgacgatag actcagcggc ctctgcaccc gcagaacgta aaagatcgac
     8221 ctgcgtggcg tcgccgtaat aaactttgta gccatattta cgcatcaggt taacggcgct
     8281 gatatcccgc tccagcacgg taatgcgcat tttatttgcc atcagcaaac gaccaatcac
     8341 ctgaccaaaa cgcccgaagc ccacgacaat gacctggggt ttatcatcgt tgacccacgg
     8401 tttttcgtct tcttcttccg gtccgttaaa ctggcgggat agccatttat ccaccagctt
     8461 catcagcaac ggcgtggtca tcatggaaag cgtcaccgtc accagcaaca gcgccatctg
     8521 gtcgccctgg aataaacgtt gtgaagaagc ggtagaaaag aggacaaagg caaactcacc
     8581 gccctgactc aacacgccag caaactgcat ccgctctgag ctacgcacgc catacaatcg
     8641 cgccagcaga tacagcacga gaattttcac cgccaccagc acaaccacgc ttatcactac
     8701 ccacaacaga tgggtataaa gcaccccgag gttgagcgac atgccgacag agataaagaa
     8761 caaaccgaga gcaatccttt gaagggatcg atagccgttt ccagttcatg gcgatattca
     8821 ctttccgcca gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca
     8881 tccataaaca atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc
     8941 acgccagaag ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc
     9001 atgccgacaa acgccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc
     9061 gaacccgcca acaacggcac taacgccagt gccgggatca ccgccagatc ctgaaacagc
     9121 agtaccgaaa acccgagctg cccggattcg ctgcgattca tccctttctc acgcatcaat
     9181 tgcaacgcca ttgcggtcga agacatcgca aggccaatgc caccgaccac cgccgcctgc
     9241 caggcgaaat ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt
     9301 gccgcgccta cgccaaaaat cgaacgccgc agttgccaaa gcttggaggg attcaactca
     9361 aggccgatga taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg
     9421 acgtcgctaa taaaccccag cccccacggg ccaattgcaa tccctgccag caaatatccc
     9481 aacacagcgc caatccccag ccgcgatgcc aacggcaccg cagccaccgc cgcgaagaga
     9541 aacagcactc ctgcgagtaa aaaatcggaa ccttccatca gcggcctcct ggagacagcg
     9601 gatttgccag ccagtcaccg taggctctgg cgtggctcgc cagctcctgt gcgctttgcc
     9661 gtctcgccca gtaaataatg atgggactta accaatgcat ccggcacatg cccgccgcca
     9721 gttcaaaggg acgcagaacg tcgctcatgg ggtagcgatt caatgcgtca taacggtaag
     9781 cactttccgg ctcgccggtg gtgatcacgc tacgccagta ctttcccgcc agttggtttc
     9841 ctcccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9901 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9961 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
    10021 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcaaaca gcagcaaaac
    10081 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10141 gggctaccat tgcgcccggt gcggcagctc gcccatacat tatcataatg ataagttaac
    10201 atagtctgaa catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg
    10261 tgcgcgtcct gctggataat gccaccgcca ccatcaaccc tgggcagaaa gtcggcctgg
    10321 tgggtaaaaa cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg
    10381 ccgacggcgg cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa
    10441 cgccggcgtt accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc
    10501 aactagaagc gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca
    10561 ttcatggcaa gctggatgct attgacgcat ggagtattcg ctcccgtgcc gccagcctgc
    10621 tgcacggcct cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttctccggtg
    10681 gctggcgtat gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc
    10741 tcgacgaacc gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggctga
    10801 agagctatca gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg
    10861 tcgataaaat tattcatatc gaacaacaaa gcatgttcga gtacaccggc aactacagtt
    10921 cgtttgaagt acagcgcgcc acccgtctgg cgcagcaaca agcgatgtac gaaagccagc
    10981 aggaacgcgt agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag
    11041 cgaagcaggc ccagagccgc attaagatgc tcgagcgtat ggagctaatt gcccccgcgc
    11101 acgtcgacaa cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac
    11161 tgaagatgga aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac
    11221 tgaacctggt gcccggctcg cgcattggtc tgttaggccg caatggcgcg ggtaaatcga
    11281 cattaatcaa actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga
    11341 aagggatcaa actcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat
    11401 cacctattca acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact
    11461 acctcggcgg ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctccg
    11521 gtggggaaaa agcccgcctg gtgctggcat taattgtctg gcagcggccg aatctgctgc
    11581 tgctcgacga accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat
    11641 taatcgagtt tgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca
    11701 ccactgacga tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg
    11761 aagattatca acagtggttg agcgacgtac aaaagcagga aaaccagacc gacgaagcgc
    11821 caaaagagaa tgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagctgagc
    11881 tgcgtgcgca aacccagcca ctgcgtaaag agattgcccg tctggaaaaa gagatggaga
    11941 agctgaacgc gcaactggcg caggcggaag agaaactcgg cgacagcgaa ctgtatgacc
    12001 agagccgtaa agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc
    12061 tggaagagtg cgaaatggca tggctggaag cccaggagca gcttgagcag atgctgctgg
    12121 aaggccaaag caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag
    12181 tgctgaattc atccccatgc gcggctctag caattgtcat ctgcaaacca tgctgccgcg
    12241 tctgtttcgt cgtcaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg
    12301 cgattttgtc gatcttgcgt ggagtgaaaa ccctgcacag gcgcaacata aacctcgtct
    12361 ggtggtgttt cacgggctgg aaggcagtct caacagccct tacgcccacg gtctggttga
    12421 ggcggcgcaa aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga
    12481 accaaaccgt atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt
    12541 acgctggctg caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg
    12601 cggtaatatg ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc
    12661 ggtgattgtc tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt
    12721 ttcccgcgtt tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct
    12781 ggcagcctac cccggaacgc tgccgattaa tctcgcacag ttaaaatcgg tacgtcgcat
    12841 ccgtgaattt gacgatctca tcaccgccag aattcacggc tacgccgacg ctatcgacta
    12901 ttatcgtcag tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat
    12961 tcacgccaaa gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc
    13021 cccgcaggtg gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac
    13081 attacttcat ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct
    13141 ggaggcgaaa tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta
    13201 attgaaagct ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa
    13261 cagaaagtcg ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg
    13321 gagctgcacg aaacggtcaa tattatgccg cgcagtcagt ttcgcgaata acctgcaacc
    13381 gtcggcggac gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc
    13441 cggtcattgc ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc
    13501 gtaaaatatt cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc
    13561 gttacacccg cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata
    13621 tcagctactt cggccccgag gctaacgact tcggcctgct ggaacaaacc ttcattgaat
    13681 acggtcagag cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac
    13741 cgtggaatca ggtaccgggg acattcaccc cctggcaacc tttaccggaa cccactgatg
    13801 tactgtttta tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc
    13861 atgtggactt actggtcggc gtggtgccta tcgttaacct tgagtggatt caaaaactga
    13921 tccgcgacac cagcgagcgc gggcactcac gagaagcagt gatggactca gtagtgcgtt
    13981 caatggaaga ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc
    14041 agcgcgttcc caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctcg
    14101 atgaaagctt tgtggtgatc cattttcgta atctggaagg gatcgatttc ccctggctgc
    14161 tggcgatgtt gcaaggctca ttcatttccc acatcaatac gttagtggta ccgggcggca
    14221 aaatgggtct ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa
    14281 agaaaatcga gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga
    14341 tagttaagtc acacggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc
    14401 atcaacgcca ctgaacaata tttctcggca gagagatcaa ccgcacgcgc aaccgctgcg
    14461 tctttcaggt cgcgaccggt gacgataaaa tgcagattaa tgtgcgtaaa caggcgtggt
    14521 gcctcttcgc ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct
    14581 ttttgcagga tcgaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt
    14641 tccatcggac ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg atgaccagag
    14701 gcggattcgc ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct
    14761 actccactgc gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag
    14821 gcgacctggg tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa
    14881 cagtcaggat gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta
    14941 ccgtgcagta cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag
    15001 agacagcggc gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg
    15061 cttggcaaac cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat
    15121 aagtacccat ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac
    15181 atcgttaaag gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc
    15241 tcctatctga atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa
    15301 cgtagcgcat gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa
    15361 tttcgccaat tgattcaggt aaacccggac attctgatgc gtttgtctgc acagatggcg
    15421 cgtcgtctgc aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc
    15481 cgcattgcac agactctgct gaatctggca aaacaaccag acgctatgac tcacccggac
    15541 ggtatgcaaa tcaaaattac ccgtcaggaa attggtcaga ttgtcggctg ttctcgtgaa
    15601 accgtgggac gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa
    15661 accatcgtcg tttacggcac tcgttaatcc cgtcggagtg gcgcgttacc tggtagcgcg
    15721 ccattttgtt tcccccgatg tggcgcagac tgatttatca ccccgatatc aactatgcac
    15781 ttcgacaaac gctggtgcta tgtttgcccg tggccgttgg gttaatgctt ggcgaattac
    15841 gattcggtct gctcttctct ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc
    15901 ctcataaacg ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgcc acctgtagct
    15961 tactgacaca gctgctactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa
    16021 cgctggtact tggcgtcact gctgagttgg ggccattgca cgcaaaattg cttcccgcat
    16081 cgctgctggc agccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt
    16141 tgctcatcta tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga
    16201 tctggcgcga acaaccgctg cgcgagtcac taagtctgct gtaccgtgaa ctggcagatt
    16261 attgtgaagc caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc
    16321 cgctgctggt gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc
    16381 atatgctttc cgcgcaaaat aatactgact acaagcggat gctgcgtatt ttccaggagg
    16441 cgctggattt acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc
    16501 tggtcgagcg tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc
    16561 gcctgcgcgt gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg
    16621 aaaagcaaat tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc
    16681 aattctgcta ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct
    16741 atgcccgtga cttactggcc gataaacagc ggcgaatgcc attacttccg gcgctgaaaa
    16801 gttatctgtc actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa
    16861 gcgttgccag cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga
    16921 cggtattgct ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct
    16981 ccgtgggaac cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg
    17041 aaggttacac cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca
    17101 aaaactacgg ctgggcgacg gtcggtttta ctattaccgc agtgtatacc ctgcaactat
    17161 tgtggttgaa cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt
    17221 taattgcttt cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta
    17281 aaaacgccca tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg
    17341 atccgcaacc tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc
    17401 tgtataactc attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag
    17461 atatgaaact gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca
    17521 cgctggcgcg ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt
    17581 gtgaaatcgc cattcagcgt tgtcagcagc gactggagta tgacgaaccg ggtagttctg
    17641 gcgatgccaa tatcatggat gcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc
    17701 tggagcagca tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga
    17761 tggcatggcg tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga
    17821 aggcgtaatg caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc
    17881 ctgatgcttc gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc
    17941 ccttcatcga tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt
    18001 ccggcattca gcaccattac gccagcctct gcgcccgcat acaggaaatc acgcgcccga
    18061 cctttgtact gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta
    18121 aacacatcgt actgctgatc gatcttctgc agatggtcaa caaaacgctg gcgtttcgcc
    18181 tgaatgcctt ccagcacttc aggggtattg atgatatcaa acgccgcccc cgctactgca
    18241 caggccagag gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca
    18301 atttctgccg tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag
    18361 gtcagaatat ccggcgtcac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc
    18421 atcccgcact gtacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc
    18481 aagccctgca aaaactctgg cgtcgctgcc gtcacaccgc cctcgccctg gatcggctca
    18541 accaccaccg cacaggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaaa
    18601 ggaacgtgga tgatgtctgc cggttttggc ccaaagccgt cggaatattt tggctgcccg
    18661 cccaccgaga cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg
    18721 gttttgaacg gactatgacg cacacaggcg taatggcgtg ccagtttaaa ggcggtttcg
    18781 ttagcttccg tgccggagtt cataaacacc acgcgttcgg caaacgtcgc ctcaatcagt
    18841 ttgcgcccaa gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagagtt
    18901 tcgccctggg tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact
    18961 gcaatgccac ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc
    19021 tgacctttta ccggaatgaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa
    19081 gtcgcgcgtg taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa
    19141 ataaatgtga aattataatc acaaaatatg cataaaaaat cattgaatgg caatcagaaa
    19201 tcagcgatgc aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg
    19261 atggaactgc acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt
    19321 ttcgctccag gccgtcacgt caaagcatgc tggtaatgag ccaggttcca ccaccagcga
    19381 atggtagcgt gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgttatg
    19441 tgtaatcggc gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt tgccgccaaa
    19501 tgcctgcgcc attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata
    19561 gtggcgaata acgtcaaggg agatcccggc ttcatctggc gtacaggggc cgggtgatat
    19621 gacaattttt tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt
    19681 aaccagcaca tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc
    19741 gtagttatct ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact
    19801 cagattcccc ggcttcgctt accactttgc taaatatcat ctgcagcgcg gtgagatccc
    19861 ccattgcccc actctgattt gcctgattcc aggcctcttt ttcgataccc tgccagctca
    19921 gttgataccc ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agaccacttc
    19981 ccacccggaa gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga
    20041 acttcgcttt ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat
    20101 tgccctcttt ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa
    20161 cttcacgcag ttgccccgcc cagtcgaaaa tatcctgata cagctggcga tggatagtgc
    20221 gcaaatgcgg taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg
    20281 tcatttcgta agcggcctgt tccagccgct gctgctggtg gatgttcagc cggttacgca
    20341 tgatatcaag gccaggataa agatacggat cgcgcccttc gccgaattta tcgctcatag
    20401 tgcctcctca gctcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaaggc
    20461 atctcgacgc cttcaagacg gcgactggcc tggaaattac gattacgctg aagctcccag
    20521 agacgggact tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat
    20581 ttgccacaag tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga
    20641 ttgcccgcgc gaaaaatcat tacggcagga ctttagcgga aaggataact accggttttg
    20701 acggcacatt ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcaa
    20761 cgtccatgcc tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccatggt
    20821 caaggaaggc gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag
    20881 cggtacgcgc catcgcgatg gtgccacgcg tgttgcgcag gccgttatcg gcttcatttt
    20941 tgattggcgg gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca
    21001 taaagccagg aatgacgcgg tgaaaggtag tgttgttata aaaaccgctg ttcacataat
    21061 cgacaaagtt ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac
    21121 cagctgaggt tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa
    21181 gagcagaaag agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc
    21241 cttaacaaac agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa
    21301 ggccatccct ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt
    21361 attccgtgat caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt
    21421 taaacatatc acaaaatcac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt
    21481 tatttttgaa cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag
    21541 ctttctctcc tacgcactga ctggtgcgtt ggttattgtc accgggatgg tgatgggaaa
    21601 tatcgccgat tatttcaatc tgcctgtttc cagtatgagt aacaccttca ccttcctcaa
    21661 cgccggcatt ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa
    21721 aacgcagtta cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag
    21781 ccacagcctg gcgctgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac
    21841 catgtcgatt ggtacattcc tggtaacaca aatgtatgaa gggcgtcagc gcggttcccg
    21901 cctgttattt accgactcct tcttcagtat ggctgggatg attttcccaa tgatcgccgc
    21961 gtttctactg gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta
    22021 tgtcgctatt tttattctga ccttcggctg tgagttcccg gcgctgggca aacatgcgcc
    22081 aaaaacggat gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt
    22141 tgcggcgctg tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc
    22201 caaaggcctg ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc
    22261 atacatggtc ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat
    22321 tctgaccgta ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc
    22381 agcacatatg gcgtggtcaa ttctcgctct gggcttcttc tccagcgcga tctataccac
    22441 catcatcacc ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt
    22501 cctgacctgc ggaaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga
    22561 acatagcggt ccgcaggcgg cactgcttac agcaaacggt ctgtacgctg tcgtctttgt
    22621 gatgtgcttc ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca
    22681 ttaattgctc atgccggacg gcactctcgt cgtccggcct tttcctctct tcccccgcta
    22741 cgtgcatcta tttctataaa cccgttcatt ttgtccattt tttgcacaaa catgaaatgt
    22801 cagacaattc cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta
    22861 tataaaggtg aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg
    22921 taatagaaaa gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg
    22981 tatggtcggc catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga
    23041 tattaccgtt ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta
    23101 cttctctcac cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca
    23161 cggcatcaaa gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat
    23221 tcactccagc gccgggcgca ccgtttttta tgacaagctg atcatggcga ccggttccta
    23281 cccgtggatc ccgccaatca aaggttctga tactcaggac tgctttgtct atcgcactat
    23341 tgaagacctc aacgccattg aatcctgcgc ccgtcgcagc aaacgcggtg ccgttgttgg
    23401 tggcggcctg ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca
    23461 cgttatcgaa tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca
    23521 gctgcgtcgc aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga
    23581 gattgtgcag gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact
    23641 ggaagtcgac tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca
    23701 gtgtggtctg gacgttgctc cgcgtggggg tatcgtcatt aatgattcct gccagacttc
    23761 cgatccggat atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct
    23821 ggtagcacct ggctacaaaa tggcgcaggt cgccgttgac catattctcg gtagcgaaaa
    23881 cgcctttgaa ggtgctgacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg
    23941 tattggtgat gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag
    24001 taaagagatc tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt
    24061 actggtgggc gataccagcg actacggcaa cctgctgcaa ctggtgctga atgctatcga
    24121 gctgccggaa aacccggatt ctctgattct gccggcgcac tcgggtagcg gtaagccgtc
    24181 tatcggtgtt gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa
    24241 aggtgatctg attgctgcca tcaacaaagg ctgccacacc gttgcggcgc tgaaagctga
    24301 aaccaaagcg ggtactggct gcggtggctg tatcccgctg gttactcagg tactgaacgc
    24361 ggaactggcg aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc
    24421 gcgtcaggaa ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct
    24481 ggcgaaacac ggcaaaggct acggttgtga agtttgtaaa ccgaccgtcg gttcgctgct
    24541 ggcctcctgc tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa
    24601 cgacaacttc ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc
    24661 gggcggtgaa atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa
    24721 tctctacacc aaaatcaccg gctcccagcg cctggcgatg tttggcgcac agaaagacga
    24781 tctgccggaa atctggcgtc agctgattga agctggcttc gaaaccggtc atgcctatgc
    24841 gaaagcactg cgtatggcga aaacctgcgt gggtagcact tggtgccgct acggtgttgg
    24901 cgatagcgtc ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca
    24961 caaaatgaag ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga
    25021 cgtgggtatt atcgccactg aaaaaggctg gaacctgtat gtttgcggta acggcggcat
    25081 gaaaccgcgt catgcggatc tgctggcagc agatatcgat cgcgaaacgc tgatcaaata
    25141 tctcgaccgc ttcatgatgt tctacatccg taccgccgac aaactgacgc gtaccgcgcc
    25201 gtggttagaa aacctcgaag gcggcatcga ttacctgaaa gcggtgatca ttgacgacaa
    25261 actggggctg aacgctcatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg
    25321 tgagtggact gaaacggtca atacaccgtc cgcgcagact cgcttcaaac acttcatcaa
    25381 cagcgacaag cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc
    25441 aacgccgtat gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa
    25501 gacatctgca aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggcgac
    25561 gagcaggtcg cgattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatc
    25621 gatccgttct tcgagtccag cgtgctgtca cgcgggctga ttgcggaaca ccagggcgag
    25681 ctgtgggtcg ccagcccgct gaaaaaacag cgttttcgct taagtgatgg cttgtgcatg
    25741 gaagacgaac agttttccgt caaacattac gaagcacgag tgaaagacgg cgtggtgcag
    25801 ctgcgcggtt aatgttttaa cgggaggcgc aatgcctccc ctttttgcat ggtcctgtaa
    25861 tgaccttcgg tataatgcag gacatttttt aaactttttg ttttattttt tgtttttatt
    25921 ttttaaagga taatcaaatg tttacagaca ctattaataa gtgtgcggct aatgctgcac
    25981 gcattgcacg cctgtcggca aataacccgc tcggcttttg ggtcagctcc gccatggcgg
    26041 gcgcatatgt gggtcttggg atcatcctga ttttcacgct cggtaatttg ctcgacccgt
    26101 ccgtacgtcc gctggtgatg ggcgcgacct ttggtatcgc cttaacgctg gtgattatcg
    26161 ccggttctga actgttcacc ggacacacca tgttcctcac ctttggggta aaagcgggca
    26221 gcatcagcca cgggcaaatg tgggcaatcc tgccgcaaac ctggctgggt aacctggtcg
    26281 gttccgtctt cgttgccatg ctctatagct ggggcggcgg tagcctgctg ccggtagata
    26341 ccagcatcgt tcactccgtc gcgctggcta aaaccactgc accggcaatg gtactgttct
    26401 tcaaaggtgc attgtgtaac tggctggttt gcctggcaat ctggatggcg ctgcgcactg
    26461 aaggggcggc gaaatttatc gctatctggt ggtgtctgct ggcatttatc gcgtccggct
    26521 acgagcactc tatcgctaac atgacgctgt tcgcgctctc ctggttcggc aaccacagcg
    26581 aagcctacac gctggcgggt attggtcata acctgctgtg ggtgacgctg ggtaatactt
    26641 tatcaggtgc cgtattcatg ggattgggtt attggtatgc tacgccgaaa gcgaatcgtc
    26701 cggttgcgga caaatttaat caaactgaaa cggctgccgg ttaattacta aggggttttt
    26761 acgtggatca tttgcctata ttttgccaat tacgcgatcg cgactgtctg attgtcggcg
    26821 gtggtgatgt cgcggaacgc aaagcaaggt tgctgttaga cgcaggcgct cgcttaacgg
    26881 tgaatgcatt agcgtttatt ccacagttca ccgcatgggc agatgcaggc atgttaaccc
    26941 tcgtcgaagg gccatttgat gaaagccttc tcgacacctg ctggctggcg attgcagcga
    27001 cggatgatga cacgcttaac cagcgcgtca gcgaagccgc tgaagctcgt cgcatcttct
    27061 gtaacgtagt cgatgcgccg aaagccgcca gctttattat gccgtcgatt attgaccgct
    27121 caccgctaat ggtagcggtc tcctctggcg gcacctctcc ggttctggcg cgcctgttgc
    27181 gcgaaaaact cgaatcactg ctgccgttgc atctgggcca ggtggcgaaa tatgccgggc
    27241 aattacgcgg gcgagtgaaa caacagttcg ccacgatgag tgagcgtcgc cgtttctggg
    27301 agaaattgtt cgttaacgac cgcctggcgc agtcgctggc aaacaacgat cagaaagcca
    27361 ttactgaaac gaccgaacag ttaatcaacg aaccgctcga ccatcgcggt gaagtggtgc
    27421 tggttggtgc aggtccgggc gatgccgggc tgctgacgct gaaaggactg caacaaattc
    27481 agcaggcaga tgtggtggtc tacgaccgtc tggtttctga cgatattatg aatctggtac
    27541 gccgcgatgc tgatcgcgtt ttcgtcggca aacgcacggg ataccactgt gtaccgcagg
    27601 aagagattaa ccagatcctg ctgcgggaag cgcaaaaagg caaacgcgtg gtgcggctga
    27661 aaggtggcga tccgtttatt tttggccgtg gtggcgaaga gctggaaaca ctgtgcaacg
    27721 cgggtattcc gttctcggtg gttccgggta ttaccgcagc ttctggttgc tctgcctatt
    27781 cgggtattcc gctcacgcat cgcgattatg cccagagcgt gcgcttaatt accggacact
    27841 taaaaaccgg tggcgagctg gactgggaaa acctggcggc agaaaaacag acgctggtgt
    27901 tctatatggg gctgaatcag gctgcgacta ttcagcaaaa gctgattgaa cacggtatgc
    27961 ctggcgaaat gccggtggca attgtcgaaa acggaacagc agtcacgcag cgcgtgattg
    28021 acggtacgct cacgcaactc ggtgaacttg ctcagaaaat gaacagtcca tcgctaatta
    28081 ttattggtcg ggttgttggc ctgcgcgata aactgaactg gttctccaac cattaattta
    28141 acccgggcca gagaattctg gccttcttaa tgactctttt tatatattca gcaaataaaa
    28201 catagcccct taataaatat tatctgctaa tgggttatat tcaccgttac agcgacttac
    28261 aaaattaagt cattcgctca ctgacgccaa ttaaatattc ctgtatcgtt cttaacgagc
    28321 agtctgctta tattcatata atcaatgaat attaattaat atataataca tagggaatgt
    28381 aaatgaacaa atttattaaa gttgcactgg taggtgcagt actggctacg ttaactgcat
    28441 gtactggtca tattgaaaac cgtgataaga actgctctta cgactacctg ctgcacccgg
    28501 caatttctat ttctaaaatc attggcggtt gcggtcctac tgcacagtaa gtctctgaca
    28561 gaaatcggct aacaccggtt taattgcccg acgccctctg gattttccga gggcgtattt
    28621 gtttctatgt ccttcttgcc cccgcccgta aaaatatttt cactttaaat tcaatttgat
    28681 aactacatcc attagccaca gcgcgatcct gtgcgaaatt ttgtgatctt cctccacatt
    28741 acataatatc atatgttgtt atattcatca tgcattgtca tgttaccttt taaatgactg
    28801 caaactctcc cctacaacgt attggacaag aaaaaggtat cgctatggga agccaggaac
    28861 tccaacgcaa gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca accgtcggct
    28921 ccggtatttt tgtatctgtg ggtgaagtgg caaaagcagc gggcacgccg tggcttacgg
    28981 tgctcgcgtt tgtcattggc gggttaattg tgatcccgca aatgtgcgtc tatgcggaac
    29041 tatccaccgc ttatccggaa aatggcgcag attatgttta tctgaaaaat gccggaagcc
    29101 gaccgctggc tttcctctcc ggctgggcca gcttctgggc caacgatgcg ccgtcattgt
    29161 cgattatggc gctggcgatt gtcagcaatc ttggcttttt aacgcctatc gatccgttgc
    29221 tcggtaaatt tatcgccgcc ggattaatta tcgcctttat gttgctacac ctgcgctccg
    29281 ttgaaggcgg cgcagcgttt cagacgctaa ttaccatcgc caaaattatc ccgttcacta
    29341 tcgtcattgg ccttgggatc ttctggttta aagcggagaa ttttgccgcc cctaccacca
    29401 ctgcgattgg cgcaacgggc agctttatgg cgctgctggc ggggatctct gccaccagtt
    29461 ggtcgtatac cggcatggcc tctatctgtt atatgaccgg cgaaattaaa aaccccggaa
    29521 aaaccatgcc acgagcgctg attggttcct gtctgctggt tctggtgctc tacaccctgc
    29581 tggcgctggt gatttccggc ctgatgccct tcgacaaact cgccaattct gaaacgccga
    29641 tttccgacgc cctgacctgg atccccgcac tcggcagcac cgctgggatc tttgttgcca
    29701 tcacggcgat gatcgtcatt cttggttcgc tttccagctg cgtgatgtac cagccgcggc
    29761 tggaatacgc gatggcgaaa gacaacctgt tctttaaatg cttcggccat gtgcatccga
    29821 aatacaacac gccggatgtc tccatcatcc tgcaaggggc gctggggatc ttcttcatct
    29881 tcgtttccga tctcaccagc ctgctgggtt atttcaccct ggtgatgtgt ttcaaaaata
    29941 ccctcacctt cggctccatc atctggtgtc gtaaacgcga cgattacaaa ccgctgtggc
    30001 gtactccggc tttcgggctg atgaccaccc tcgccattgc gtcaagcctc attctggtcg
    30061 cctcaacctt tgtctgggca ccgattcccg gccttatctg cgccgtcatc gttattgcta
    30121 ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg ccagctcaac gctttgtcgt
    30181 aatgttatct ggagaaaata aaatgttgga tattgataaa agcaccgtgg actttctggt
    30241 caccgaaaat atggttcagg aagtggaaaa agttctcagc catgacgttc cgctggtgca
    30301 caccatcgtg gaagagatgg tgaagcgcga cattgatcgt atttatttcg ttgcctgcgg
    30361 atcgccactc aacgcggcgc aaacggcgaa acatctggcg gatcgctttt ccgatcttca
    30421 ggtctacgcc atttccggct gggagttctg cgataacacc ccgtatcgcc tcgacgatcg
    30481 ttgcgcggtg attggcgttt ctgactacgg taaaacggaa gaggtaatca aagcgctgga
    30541 gctgggccgg gcctgcggcg cactcactgc ggcgttcacc aaacgcgcgg acagcccgat
    30601 tacctcggcg gcggaattta gcattgatta tcaggccgac tgtatctggg aaattcacct
    30661 gctgctctgc tacagcgtgg tgctggagat gatcacccgc ctcgcgccga acgcggaaat
    30721 cggcaagatc aaaaacgatc tcaagcagtt gccgaatgcg ctcggtcaac tggtacgcac
    30781 ctgggaagaa aaaggccgcc agcttggtga actggccagc cagtggccga tgatttatac
    30841 cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa ggcattgtaa cgctgatgga
    30901 atttacctgg acgcacggct gcgtgattga gagcggagag ttccgccatg gcccgctgga
    30961 gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc aatgatgaaa gtcgccacac
    31021 caccgaacgc gccattaact ttgttaaaca gcgtactgac aacgtgatcg tcatcgatta
    31081 cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt tcgtgccaat
    31141 ggagtggctc tgctactacc tgtctattta caaagatcac aacccggatg aacgccgcta
    31201 ttacggtggt ctggtggaat attaatccct ctcccggccc ggcaacgggc cgaatttacg
    31261 caaggagtta cccgatgaaa acaggtatgt ttacctgcgg ccaccagcgg ctgcctattg
    31321 aacacgtatt tcgtgatgca agcgagctgg gttacgacgg catcgaaatt tggggcggtc
    31381 gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat caaacaaatc aaggcgctgg
    31441 cgcagacgta tcagatgccg attatcggct atacgccaga aaccaacggc tatccgtata
    31501 acatgatgct gggcgatgaa catatgcgtc gcgaaagcct cgacatgatc aagctggcga
    31561 tggatatggc aaaagagatg aacgcgggtt atacgctgat ttccgcggcc cacgcgggct
    31621 atctcacgcc acctaatgtt atctggggac ggctggcaga gaacctaagc gaactgtgtg
    31681 agtacgcgga aaacatcggc atggatctaa tcctcgaacc gttaacgccg tatgaatcga
    31741 acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc gctggtgcct tcgccgcgcc
    31801 tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg atgagttatt
    31861 tcgacaaact gggcgataaa ttacgtcatc tgcatattgt cgacagcgac ggggccagcg
    31921 acacgcatta cattcctgga gaaggcaaaa tgccgctgag ggaactgatg cgcgatatta
    31981 tcgaccgggg ctatgagggt tactgtacgg tggagctggt gacgatgtat atgaacgagc
    32041 ccagactcta tgcccgccag gcgctggaac gctttcgcgc gctgctgccg gaggatgaga
    32101 gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat atctacccgc aactgaataa
    32161 agcgttttct ggcggtaatg cggtcaatat ggcggtgtac tgcactcgct acggcataca
    32221 gccgggatgc attacctggg tgggtgacga tgactacggc acaaagctga agcaggatct
    32281 cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa cacggcgtta ccgcacaaac
    32341 tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac tacaccgaag gcgtgatggc
    32401 cgactttgcc ctgagtgaag aggattacgc ctggctggcg cagtatgaca ttgtgcacgc
    32461 ggcaatctgg ggacatgcgg aagacgcatt cccacagctg cacgctgcgg gcaaacttac
    32521 cgctttcgac ttctccgaca agtgggacag ctcgctctgg cagacactgg tgccgcatct
    32581 cgattttgcc tttgcctccg caccgcaaga agacgaagcg ctgcgtctga agatgaaagc
    32641 gattgttgcc cgtggcgcag gcgtggtgat tgtcacgctg ggtgaaaacg gcagcattgc
    32701 ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg gtgacggtta tcgacaccat
    32761 gggtgccgga gattcgttca ttgccggatt cctttgcggc tggtctgcgg ggatgacatt
    32821 accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa accattcagt accacggtgc
    32881 ctggtaggta taacgttggc gtgagcatct tcacgccaac gtgctgttac ttgccggaaa
    32941 acgaccctat aatccgagta attcattctt tatttcaggg tcgattatgt cagctacgga
    33001 ccgctactct catcaactcc tctacgctac cgtccgccag cggttgctgg atgatatcgc
    33061 gcagggggtt taccaggccg ggcaacagat ccctaccgaa aacgagcttt gtacgcaata
    33121 taacgtcagc cgcattacta ttcgcaaagc tatcagcgac ttagtggcag acggcgtact
    33181 gatccgctgg cagggcaaag gcacctttgt acaaagccag aaggtcgaaa acgccctgct
    33241 gaccgtcagt ggttttaccg attttggcgt ctcacaaggc aaggcgacga aagagaaagt
    33301 gatcgagcag gaacgagtca gcgccgcgcc gttttgcgaa aagctgaaca tccctggcaa
    33361 cagcgaagtg ttccatcttt gccgggtgat gtatctcgat aaagagccgc tgtttattga
    33421 tagttcctgg atcccactgt cgcgttatcc tgactttgat gagatttacg ttgaaggaag
    33481 ctccacttat cagttatttc aggagcgttt cgacacgcga gtggtcagcg acaaaaagac
    33541 catcgatatc tttgccgcca cccgcccgca ggcaaaatgg ctgaaatgcg aactgggcga
    33601 accgttgttt cgcatcagca aaatcgcctt tgatcagaat gacaaaccgg tgcacgtctc
    33661 cgaactcttc tgccgcgcca atcgcatcac cttaacgatt gataataaaa gacattaacc
    33721 gtaggccgga taagatgcgc cagcatcgca tccggcgatg ctggcgcgtt gaattttaca
    33781 tcccgtacgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcagtac
    33841 ggaatttagc ttgacggtgc tatccagcat taaatactgg caatactctc acgcaaaatc
    33901 cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca atgttctgat
    33961 tgtggattcg cctggcgaaa cgttccggaa aggcgataaa agagcggcgg gatttcatat
    34021 ttcgactctg cacccaccgg gtaaggcaag gcaccgcgct tttgcgcctc ttccagcact
    34081 ctggcggcaa tcggctgatg aaactcgaca atcaacacct tcgactgcgc attagcaatc
    34141 accgcgcttt tcacttccgg caccgcacca ccgttaagca aagccagcaa ccgttcagat
    34201 acccctgcct gcaccgcgtg catcactggc gcaaacacca gaccacgcaa tacttccagc
    34261 gcctgtgcgc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat acggttgata
    34321 acatcagcat cgccgaccac tgcaccaaca ccctctggcc caaatagctt gaagcaggaa
    34381 aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ccaccttcat caccgcatag
    34441 ttgtcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac atctgccagc
    34501 acgtagctgt cctgcggctg ctggcgcgta tgctgcacca gcgccgcatc cggttgttgc
    34561 tcgtcgacga cctgcttcag tgccgacagg tcattgaaat caacagtaat aagcgtcagc
    34621 cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg caccagaaga
    34681 cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt ccccgcgccc
    34741 tgcaccagcg ccgcagcctg tgcgtgaaat gcatcagcca gcacctgctc cacccgttgg
    34801 gtaatacgcg gttgattcag acctggagtt aaccctaaat caccgccagt aagaaactcg
    34861 ctgccgggga aatggcgaca aatgctatcc accagcgcaa actgcttttg ctgcgcctca
    34921 ataatcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa gccggaacga
    34981 acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact gccacgaccg
    35041 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
    35101 cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa ccgaccagca
    35161 gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
    35221 acgggtagcg tcccagccat ttgccgatcg aacgaagcag caagacttcc gccgaaataa
    35281 ccactgcgcc taataccgcc gcaaccatcg gatttggcga gagatagccc accgcataaa
    35341 caaaggtaaa gcccgcaact gcatacacgc cagtggctaa cgcggtggtg gcaatcaacg
    35401 gcacaaaacc cagtccgcgc ataaattctg ccagagccgc ctgattaatc agcgtttgcg
    35461 attgttccgg cgtcacgcct gcggaatacg ctttctccag tgtgaagatc gaaacttcgc
    35521 taccagcaaa aatcttcata ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
    35581 aaggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc ccgctggcat
    35641 cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
    35701 ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacta
    35761 ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag ccaaactggt
    35821 agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag cttaattccc
    35881 cgaggctacc taatacatcc accggcagcg cggtcagcag ctggtttact ggcaacaggc
    35941 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
    36001 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agcccggttt
    36061 tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca aaagcgatac
    36121 tccccgcctc gcgacgattc atatagcctt caatcagttg cggcaggatc gggcggatgc
    36181 cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
    36241 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
    36301 cagcatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca ctttcccttc
    36361 agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg caatggtgca
    36421 acttttgtta tagccaatca ccgcgatggc aatcgaaaat gccgcgcccg caccggtatt
    36481 acacgcaccg atgtagtaat ccagttgccc ggatttcact ttcattgccg cttccatgtc
    36541 gttgtgaatg aaaacttcaa aactgccagg agccgtcgct tcaatggttt ttttaatctg
    36601 ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt
    36661 gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
    36721 attgcggaat aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat
    36781 gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta
    36841 gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt
    36901 cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgaga ttgtctttca
    36961 gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca
    37021 gttgttccag ccccatggtg ctgaacgaag tatgcgtgga gatcgggcgt ccggtctggt
    37081 tatgcgccag cgcagcggca ataaacacct tctcttccag cggcgtaatc ttcccttcgc
    37141 tagagccgat ctccgcgata atcccggctt tcaggtcggt gccatcgata ccctgttcaa
    37201 tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcgccaca tgctccggga
    37261 aaaaagcgtc ctggtaataa ccggtgcagg ccaccacgtt gatccccgtc tcgcgcatta
    37321 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac
    37381 gcacgccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
    37441 ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg
    37501 tgtaacccgt cggatcaata ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
    37561 agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag cccccacatc
    37621 agaaagcgtg gtgcgtacac cgagttgcgt gtggaccagc ccttgctgat aaaccagcac
    37681 tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc
    37741 catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc
    37801 aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt
    37861 ttcctggata ttgatgcaaa taaacgccgt cggatgcgtg ttaaattcgt cgagggtgat
    37921 atccataatc cgctggctat ccaccaggtt ttgccagctc acgccataag gattgctgac
    37981 gatatctgcc accttaccta ccagcacggt tggcacgccc gcctcataca gtttttgcgg
    38041 cacctgcact ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata
    38101 agcgccagaa cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc
    38161 gagaatacgc tgactgtcgg ttaacaagcc gccaaatgta atgacccgac cgacctgtac
    38221 ctgctcacgc acgatacgac caattttgat cgcgtcgtca aaagagatca cagagagatt
    38281 ggcggtaatg ttatagacct ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
    38341 ctgattgacc cacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga
    38401 aactaatgcc tgctcaacac ggccaatcac atcgcgaaag ggcatccgca gcggcggtaa
    38461 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
    38521 ctctgccacc ccccaggttg cggaatctga cggttgcata tcacctggtg cataacccaa
    38581 tgcgttgatt agccccagct tctccagcgc tggtagctgc aaatgcggca actggctcag
    38641 gatgtgacca caggtattcg ccccggcatc ttgcgggcgc accagcgtga catctttcat
    38701 tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccatta cgcccccaga
    38761 gagtttccaa gactgtcata acgaccgacg attttcggtt cgccacggtg aataccggac
    38821 accagcacca catcgctacg agtgacaaaa atctgcgtgc gaaaacagag cactactgca
    38881 ctgcttaccg gaaactcgcc tgccagcggc agggtgtagt cgatactgct gtcatccaca
    38941 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
    39001 gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
    39061 gagatttcac ttaaccaaag catcgctata tattcaggct gatcgccctg ctggtttgcc
    39121 ggaatagtgc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta ttccgccagc
    39181 atcggcagcg aagtgcagct ggtcgcagaa ggcgcgttca gttgctcaat cgcaatacca
    39241 gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt cggcaaaact
    39301 ttcccggcag cctcatccca aagcaggcaa gggaaatggg taagtccggc taaatgcaga
    39361 cccggcagtt tcttaatttc agcaaccacc tcgtgcagcg aatgctgtac aaaaccgctc
    39421 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac aaactgcaca
    39481 cgtccggcct tcaccgctgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
    39541 atgacgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat ttgtaccaga
    39601 tgcccctgat gcgccaccga caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
    39661 accgccacaa tgccgctgta acctaatgcc agcaattttt ccgccagcca tgggttacgg
    39721 ccaaactgct tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatcagt
    39781 cgcttaccgt tttccagtac ctgatccacg tcgatcaccc agctgtccgg ggcgatcttg
    39841 ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
    39901 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
    39961 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagccatcc
    40021 ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact tccagtgcga
    40081 attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
    40141 gctctgccag caattcgtta tccagcggct ctatttcttc accacggcga ctgcgcatca
    40201 gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggcagatggc
    40261 actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc ttatcaataa
    40321 cgcctgcctc gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa
    40381 ctcggttcct gttccccact cactacccgt tgttgatggg cgagcagggc gtgtttatct
    40441 acaacggcac gcagcagttg ttgcattggg taatcatcga ctcgcgtcag gaccacggct
    40501 tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcctctaa tcccagcatg
    40561 gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc tttaacaatg
    40621 cgttgtaaac tctcgtgata agagagatcg acacgttcca catcactatc gccaaaaaag
    40681 acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg cttcacgttt
    40741 gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg cggccccaac
    40801 tccagcgcga ggcaaaaccc tttttgcgtg agataacttt ccgccgccag tcgggaaacc
    40861 accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
    40921 tgcgcatagt aaaaaggaat gccatcaaac tgggctttca atccgctcgc taaaccttcg
    40981 tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc cacatgactc
    41041 agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg gcgttcaatc
    41101 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aacggatgaa
    41161 cggcattcag ttgccagctc atctatggtt ttcaacctat taccgcattt ttcacccaac
    41221 aaataacggg ccagcgtcgt gataacgacg ccttcttttt tgataaacgt tcgacgcata
    41281 atatattttc aataaaatga atgtttatat cttcattaaa atgaagataa gtggcaactg
    41341 tgagatgtgg aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa tgaggataaa
    41401 aaaaaccggg tttccccggt ttcagagtga tgacgaagtg atttattcga caatgcgagc
    41461 aaagggtaat tcccactggc gcttgcctgc tcccctcacc ctaaccctct ccccaaaggg
    41521 gcgaggggac cgtccgagcg ttttgatatt acggcttcgc cacaaaacca atcgcttcgt
    41581 acaccgcttt cagcgtacgg gaagcgtgta cactggcttt ttctgcgccg tctttcatca
    41641 cctgttgcag gaaggcttca tcgttgcgga aacggtgata gcgttcctgc aattcagtca
    41701 gcataccgga aacggcatca gccacttcac ctttcagatg accatacatc ttgccttcga
    41761 actgtttttc cagttctgga atgctctggc ccgttaccgc agaaaggata tccagcaggt
    41821 tggaaacgcc cgctttgttc tgcacatcgt agcgaactac cggcggctcg tcggagtcag
    41881 tgaccgcgcg tttgattttc ttcactaccg atttcggatc ttccagcagg ccgataacgt
    41941 tattgcggtt atcgtcagac ttggacatct tcttggtcgg ctccagcagc gacattacgc
    42001 gcgcgccaga tttcggaata aacggctccg gcaccttaaa gatctcgcca tacagcgcgt
    42061 tgaaacgctg ggcgatatcg cgactcagtt ccaggtgctg tttctggtct tcacccaccg
    42121 gtaccagatt agtttgatac agcaggatgt ccgcagccat cagcaccgga tagtcaaaca
    42181 gaccagcgtt gatgttctcg gcataacgcg cagatttatc tttaaactgc gtcatgcggc
    42241 tcagttcgcc gaagtaggta tagcagttca gtgcccagcc taactgcgca tgttctggta
    42301 cgtgggactg aacaaaaatg gtgcttttct caggatcgat accacaagcc agatacaagg
    42361 ccagcgtatc caacgtcgct ttacgcagct tctgtgcatc ctggcgcacg gtgatcgcgt
    42421 gttggtcaac gatacagtaa atgcaatggt agtcatcctg catgtttacc cactgacgca
    42481 gcgcacccat gtagttacca atggtcaatt cacctgaggg ctgtgcgcca ctaaaaacga
    42541 tgggcttagt catttttcga ttcctgattt tcgctatgcg gaagccctaa tgcgggcaga
    42601 aggtcattta tagactgata aattacatca ggctggctga gatcgatagc ctcgccgtag
    42661 ttatatccgt aggttaagcc aactgatggg caacctgccg cttttgccgc ctgaatatca
    42721 ttgcgtgagt cgccgacaaa cagcatctgt tgtggggcaa ttcccatccg ctcagccacc
    42781 agtaacagcg ggtccggatg cggttttttg ttttgcacat catcgccgcc gataaccacg
    42841 ctaaagtatt tggcaatatc taaggcttcg agcagcggcg cgacgaacgg cgtcggtttg
    42901 ttggtgacca ggcctagcgg caggccttta gcctgcaacg cgcccaacgt atcggcaacg
    42961 tgcgggaaca aaaacgtccc ctcttcggca acctcgccat agtagcgatc gaacagttta
    43021 cgcagaatac gtacctgttc ttctgccgga atgtcgtcat caacgggcgg tttacccatt
    43081 gttttacgct gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc catcagaaca
    43141 tctgcgccgt taccaatcca ggtaataacg cgttcttcac ctgcgacggg caactccagc
    43201 gcatacagcg ccatatctac cgcagcagca agaccgggag cactgtcgac cagcgtacca
    43261 tcaagatcaa aagcgacgcc gcgaatatct tcaaacttat tcatgactta cctttgccag
    43321 ttcactgcgc atttcatcaa tgactttttt gtagtctggc tggtcgaaga ttgccgaacc
    43381 ggcgacgaac atatccgcgc ccgccgcagc gatttcgcca atgttgttca ccttcacgcc
    43441 accgtctact tccagacgaa tgtcaaagcc agactcgtcg atacgacgac gtacttcgcg
    43501 cagtttatcc agtgtttgag gaatgaacga ctgaccgccg aaaccaggat tgacggacat
    43561 cagcaggatc acatccagct tatccatcac gtaatccaga tagctcagcg gtgtcgccgg
    43621 gttaaatacc agtcccgctt tacagccatt ttctttaatc agttgcagcg tgcggtcaac
    43681 atgctcggag gcttctggat gaaaggtaat gatgctggca ccagcggcag cgaaatcagg
    43741 cacaatgcga tcgacaggtt tcaccatcag gtgtacgtcg ataggggcgg taatgccgta
    43801 gttacgcaag gatttcagca ccattggccc aatcgtcaga ttgggaacat agtggttatc
    43861 catgacgtca aaatgcacga catcagcgcc agctgccagg gcttttgcgg tatcttcacc
    43921 caggcgggca aaatcagccg acagaattga gggggcaatc aaatactgtt tcatccgctt
    43981 ctccttgaga attatttttt cgcgggtgaa acgactcctg gtttgtacaa agccagcagt
    44041 tcgtccacct ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac tttgacgaca
    44101 tgcaattttg cgcgctgata ccactcacgc gttaacatcg tatcgtgatt ggagatcagc
    44161 actggaatat ggcgctcaac cagaccttcg gcgatctccg ccagatgcgc ttgttgttca
    44221 agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg tcgcagacag cggtgcataa
    44281 ggcggatcgc aatagacgac ggatgcatca tctgcgcgcg ccatgctatc ggcgtaagac
    44341 tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc tgcttccggg
    44401 aaatagggtt ttttgtagcg gccaaacggc acgttaaact caccgcgcag attgtaacga
    44461 cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg acggaacgga
    44521 tcctggcttt tgttgaactc ttcgcggaac tgatagtaga cctcggcgca atttgtttcg
    44581 ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag tacgcatctt cacaatgtta
    44641 tagagactga tcaggtcgct attgatatcg gcaaggatat aacgagaaaa gtcggtgttg
    44701 agaaacaccg acccggcacc tacaaaaggc tcaaccagac attcgccctt gggcaaatgc
    44761 cgtttaatat catcaagcag gggatacttg ccccctgccc acttcaaaaa agcgcgattt
    44821 ttcttcatgc tgactaacta attacacctt ctccggctgt ggagaaagct ccgacagcat
    44881 ccagcgcttc aggcaactcc cgcaacgata acccattgcg ggagataacc ttgattactt
    44941 cagatcggcc tgtacctgac gcagcggttt cgcccacggg tttttggcct gaacatctgc
    45001 tggcaatgta gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc cagaaaccag
    45061 gacataccac ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt tcagattctc
    45121 tttcttcgcc caaccgttca ggttgtcgta gttagaggaa ctgctcagct gcagagtgta
    45181 atggctggac ggtgccgatt tcaacgaacc aacattacct gcggtcttcc ctccagcggc
    45241 tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact ggagccgtag tcgccgtctc
    45301 ttttggtgct ggcgcagaag tcgcagccgg tgccttcgtg ctcgccacag gagcagctgg
    45361 ttcagtacgc ttcggcgtct gtgctaccgg cttcggttcc gttttcacgg ttgcttgcgg
    45421 ttttttcggt tcaatcaccg cctgctgacg agctggacgc gtagtggccg gacgttcagc
    45481 agtttgcgtt ttcgccgtgt cacgcgatgc attgccattg cgtactggcg cgacagttgc
    45541 tggttcagtc ggcaatgtag aattgaccgc cacattgttc aactgttgct gattttgtgg
    45601 ctgggtcagc gcattgttca ggtcaccctg cacttcaaca cgttgttgac catccgttgc
    45661 cgccggggtt tgcccttgag tcggcgtaga agagatcggt ggcagagaaa catcctgctg
    45721 agtattttcc gcagacgtgg ttcccggcgc tggctgcacc ccattcgcct gatcggtcgc
    45781 attgcctgca agatcaatac tcttctcgcc agacgcggtt tgatcgctgg aagaggtcga
    45841 gggggctttt agcgcagaac cgataccgat gatcaacagc agtagaacca gaatgccgac
    45901 gcccatcatc atatactgac gagaagcggg tttactggct gcttttttgc gcttacgcgg
    45961 acgacgctct acgcgctctt catccacggt ttcatcttcg gattcgtcaa tttcttcttc
    46021 gatttccggt tcctcattgc gctctttttg cgcacgagtc ggacggcgat cgtcagtgtc
    46081 atcaagttca atatcatcaa aattgatctg cggttcgcca cgttcagtac gctcagaacg
    46141 ttcagaagat tgacgagaac gaccagtacg acgatcgctg ggatcgggtt tcagctcgtc
    46201 ttctggtttg aattcatcca tttaacaccc cactaaaagg ttaatgctta ccacgttgca
    46261 attaacctga agctaataga ccgcttgata agcggcctga cctttcttgt tgttacgctg
    46321 attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga aacgccgctg cgaacttcac
    46381 tcttaccaat tgccaacgga agaattaagc gtatctctcc cgcaaggact ttcttgtcac
    46441 gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg cggcccattg accggtaacc
    46501 cagcccgctt gagcagggtt ataatacgct gcgtttcggc agaactaaac tgcccgagac
    46561 gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac cgcttcacca tgtaaccaat
    46621 tgccataccc catttcagct tcaatggcat gaccaaaggt gtgtcccaga ttcagtaaag
    46681 cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc tgccttcagt tcacaacaac
    46741 ggcgaataca gtacgccatt gccggaccgt ccagacgcaa caacgcatcc agattctctt
    46801 ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta tttgatgact tctgccagcc
    46861 ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc gagatccacc accactgaag
    46921 caggctggta gaacgcgcca atcatgtttt taccgagggg atggttgacc gcagttttgc
    46981 cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg gacttgaatg aaacgaacac
    47041 cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc cactacgccg ccgccaagcg
    47101 ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa cgccgtaaag acggtatcga
    47161 gtacagccag gcttttatac tgctcgccgt cagggaggat aacgctatcg acgttaacac
    47221 ccgcctgttc aagtacgccg cggaccttat cgagatacag aggagccagg gtttcgttgg
    47281 tgaccaacat gacctgctcg cccgatttca gcggtaagaa tgaagctggt tcattaaaca
    47341 aaccagatgc gatggtaatt gggtaactac gttccccgag agtaacgaca atcctctcca
    47401 taacgcgaca tccaccttaa ttactgtacc cgcagacgag tgtatataaa gccagaatta
    47461 gttgctttcc agcatgtgaa taatctggtt tgcaaccact ttagcgcttt gatcatcagt
    47521 acgaatggtc acgtcggcaa tctcttcata cagcggattg cgttcattgg ccaacgcttc
    47581 cagaacttca cgcggcggtg tttcaacgtg cagcaacggg cgttttttat cacgctgcgt
    47641 gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg acaacgccac gagcggaaag
    47701 acggttacgc gtttcacggg atttcacaga gccgccgcca gtagccagca caataccctg
    47761 tttctcggtc aactcattga tgaccttttc ttcgcgatcg cggaagcctt cttcgccttc
    47821 taaatcgaaa acccagccca catcagctcc ggttcgtttc tcaatctctt gatcggaatc
    47881 gtaaaattcc atattgagtt gttgagctaa ctggcgccca atagtgcttt ttccggcacc
    47941 cataggccca accagaaaga tattgcgttt ctctgccatt ttttcggtac tactaagact
    48001 attcgttaat gataaacccg cttcgctcag agagcgccgc aggacatgaa ctgaaacctc
    48061 ataagatatt gcgagagtca gactgaaaat tatctcaata ctccagcggg tttggcaact
    48121 gaataaatca cccatccctc tgcatatctg gtcgctgcaa gcgcgctgcc ttgctaccac
    48181 cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa agacagcaaa atgccgcctg
    48241 aatgataaat catcatcatg gaacgcaggc agcgaagagc accaactctc aaatcggtac
    48301 tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa acacgttcag cataaaaaca
    48361 ttaccgcttt tacggctgtt tactcactgg aaaccagtcg tggcgtgata aacaccacta
    48421 actcgcgtcg ttcatcttct tttccgtcat gacgaaataa ttgcccgaac cagggaatgt
    48481 cgccaagcaa cggtacgcta tcctgacccg atttattttt acgggtaaaa atgccgccca
    48541 gcgccaacgt ttctccgctt ttgacctcga cctgcgtttc gatctcctgc ttatcaatcg
    48601 ccagcacttc gccatcggcc tgctgtagca cctgccccgg aacgttctgg ctgatgtgta
    48661 atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt gacctccatc cccaggacgg
    48721 cctctttaaa ttccaccgac gtcgcgccac tttccccgct ggaaacctga tatggaattt
    48781 cgctcccctg tttaatgctg gcaggctgaa gatgtgaggc cagcagacgc ggactggcga
    48841 taatatccag ctgctgtttt tgttcgagcg cagaaagctc aagatccagc aagcgtccgt
    48901 tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt cgctacggag aggtcgctac
    48961 caagcgtggt gacttgccca acgccaccag cgtgttgcgc atcggccagc gtccatttca
    49021 cgcctaactc acgcaaactt ttttcattaa tggtgacaat atgcgccgac agctcaacct
    49081 gcccgaccgg cagatccatt tgcgctaccc actgttcaag cgcgcttaac gccgttttgt
    49141 tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt catactccct ttggcactca
    49201 gtagcttctc ccccgctttc gccagttctc ccgcgtcggc gtattgcagg gttatactgc
    49261 gattttccag cggcagattt gcctgcgccc gcgcctgctc cgcctcctgg cgggcgatat
    49321 tgttattctg ccaggcaatg gaatgcactg agagaatgtt gtcttcctgc cgcgttatca
    49381 gtccggcgct tttcactaca gtttgtagtg cctgcttcca gggaacatct gttagatgta
    49441 acgacaccgt accgctgacg tctggcgaca cgaccaggtt caacttctcc tgttcagcca
    49501 gcgcctgcaa cacctgagcg accggaacgt catccaccat cagcgtcact ttttgcggct
    49561 ttgccgcctg tacgccgggt atcagcatca acagtagtgc ggctatccat tgcttcattt
    49621 gtatctcctt gccgttgcca caaccattgt ggcggttcgc agtttgtccc ggttcccagc
    49681 gttagtgcgt ctggcgtcag ctgtaaaatt gtccagccgt tttccagcac atcgttttgc
    49741 tgcacccgtc gccatttctt ttgcccgtct tttattacac cgatgatgtg ctcgcctcgc
    49801 cctaccatcc cctgatagcg ccactggcta agttcgctaa tccggcatag atcttccggc
    49861 ggtttaaaag ggtcacgcat accggttaaa aggcacaatg caatacctgc caacaaccag
    49921 cgtttaaccc tcatgcggtg tctccagttg tagcgtgaac aaaagatcat caccttccac
    49981 gcttaacgaa aaacggctca cgctgacgtt gcgctctgcc agccgtgtaa atgccgacgg
    50041 cactgcttcc cacagcgttt tcaacgccaa ctcacctccc tgcgcggatg gatgccagga
    50101 aaccagttgc gcgccgggca actgaaaatc cagtggcgaa aagggcagcg ttttttcttc
    50161 gctgaaggga gtggtgtcaa ccaggcgata cagtgtggcc cactgctggt gatgacttgc
    50221 ccgtagtcga attaatgcgt cgcgctcttc atggtgtgtc gatgacagaa aaatgagcgt
    50281 aactaacatc agcaaccaga atgcccagca aaactggcgg aggcggggtg atgtggcgaa
    50341 ccaccagtca aagaacatgt tcatcgctaa ccttccttgt taactgatac tcaaattgcc
    50401 agcgtccctg cgcatcctgc tgcgtggctc cccgctgatt gagatgaaaa gacgcatcct
    50461 ggcggagtga cgtttctagt gcgtttaacg cggtaatgct tgttgtcagc cccttgatct
    50521 ccagcgttcc ctgctgccag cttatcgttg tcagccaggc gtgctcgggt aaaagcgccg
    50581 ccagtgcttc cagcgcagat tgccagtcgc gggtaaattg tcgctggcgc tgccgttgcg
    50641 aacgctgttc gcgtaactgc tgttgctcca gcaaacgtgg cttcgttatc tgtaagctgc
    50701 gggcgagttg ttgttccgcc tgaagcaaaa ctgcgtctat gcgcgcttcg gcgctgcctg
    50761 tcagacgcag tattagcgtt atcccgacgg ccagcagcag aggcgcaacg aacatcagca
    50821 accagaaacg cagaaaagcg gtccggcgtt gctgtcgcca gggcaaaaaa ttaattggcg
    50881 ggttcatcag tacgccttcc caagcgccag ccccagcgcg atggcaaagt ctccaccagg
    50941 cggcggtagc ggcggctgac gaacagaaac ggcctcccag gggtcaaatc cgccttcgcc
    51001 acatatcgcg acgctgtcgg gatcaacgga taacgctgcc gccagctcct tcgcgctagt
    51061 catccccacc gccagtttgc gcccccagct atagcgcgtc gcccacagcc actgttcgtt
    51121 atcacgccag gccagacatt gctgatgaga aggtaaaaaa ggcaggaatc gctgtaatgc
    51181 actggcatcc ggggtgatcg cactaacatg aacacgcaac ctttctgcca gagtaagcag
    51241 cgttgccagc tctttgcttt gcgcggcagt cacgttatag gcgggactga gtgagtcttc
    51301 gctgtaatcg aaacgcaggg agtccggatc catatccagc tcgcgggcca tcgtccctga
    51361 cagccaggcc gtttgctccc gctcaccaag ggacatcgac gggcgcggaa atgaccgctg
    51421 taatgtgcga ctggcgggaa acgccaacat aatgtgatga cgctgcggca gttcgcgact
    51481 ccacggtaac aacgttttag ccagctgctg cgcatcaaca atccgcccat ctttgataat
    51541 gtcgttctcc agcggcaacc gccaccagcg ttgcaaaaag cattcttttg ctccccgcac
    51601 gatcgcaacc gctaccgctt cttgctgttg taaatgcaaa ccaatttgcc agatcttaaa
    51661 tgccattgtg atgatctcct tatcacccgt cactctgacg ggtatatcaa tgcgtctggc
    51721 ttgcctttat actaccgcgc gtttgtttat aaactgccca aatgaaacta aatgggaaat
    51781 ttccagtgaa gttcgtaaag tattttttga tccttgcagt ctgttgcatt ctgctgggag
    51841 caggctcgat ttatggccta taccgctaca tcgagccaca actgccggat gtggcgacat
    51901 taaaagatgt tcgcctgcaa attccgatgc agatttacag cgccgatggc gagctgattg
    51961 ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga tcaaatccca ccggagatgg
    52021 tgaaagcctt tatcgcgacg gaagacagcc gcttctacga gcatcacggc gttgacccgg
    52081 tggggatctt ccgtgcggca agtgtggcgc tgttctccgg ccacgcgtca caaggggcga
    52141 gtaccattac ccagcagctg gcgagaaatt tcttcctcag tccagaacgc acgctgatgc
    52201 gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca gttgctgacg aaagacgaga
    52261 tcctcgagct ttatctgaac aagatttacc tcggttaccg cgcctatggt gtcggtgctg
    52321 cggcacaagt ctatttcggt aaaacggtcg accaactgac gctgaacgaa atggctgtga
    52381 tcgccgggct gccgaaagcg ccttccacct ttaatccgct ctactcaatg gatcgtgccg
    52441 tcgcgcggcg taacgtcgta ctgtcgcgga tgctggatga agggtatatc acccaacaac
    52501 agttcgatca gacacgcact gaggcgatta acgctaacta tcacgcgccg gagattgctt
    52561 tctctgcgcc gtacctgagc gaaatggtgc gccaggagat gtataaccgt tatggcgaaa
    52621 gtgcctatga agacggttat cgcatttaca ccaccatcac ccgcaaagtg cagcaggccg
    52681 cacagcaggc ggtacgtaat aacgtgctgg actacgacat gcgccacggc tatcgcggcc
    52741 cggcaaatgt gctgtggaaa gtgggcgagt cggcgtggga taacaacaag attaccgata
    52801 cgctgaaggc gctgccaacc tatggtccgc tgctgcctgc cgcagtcacc agcgccaatc
    52861 ctcagcaagc gacggcgatg ctggcggacg ggtcgaccgt cgcattgagt atggaaggcg
    52921 ttcgctgggc gcgtccttac cgttcggata ctcagcaagg accgacgccg cgtaaagtga
    52981 ccgatgttct gcaaacgggt cagcaaatct gggttcgtca ggttggcgat gcatggtggc
    53041 tggcacaagt gccggaagtg aactcggcgc tggtgtcgat caatccgcaa aacggtgccg
    53101 ttatggcgct ggtcggtggc tttgatttca atcagagcaa gtttaaccgc gccacccagg
    53161 cactgcgtca ggtgggttcc aacatcaaac cgttcctcta caccgcggcg atggataaag
    53221 gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc tcgctgggat gcaggtgccg
    53281 gttctgactg gcagccgaag aactcaccac cgcagtatgc tggtccaatt cgcttacgtc
    53341 aggggctggg tcagtcgaaa aacgtggtga tggtacgcgc aatgcgggcg atgggcgtcg
    53401 actacgctgc agaatatctg caacgcttcg gcttcccggc gcaaaacatt gtccacaccg
    53461 aatcgctggc gctgggttca gcgtccttca ccccaatgca ggtggcgcgc ggctacgcgg
    53521 tcatggcgaa cggtggcttc ctggtggacc cgtggtttat cagcaaaatt gaaaacgatc
    53581 agggcggcgt gattttcgaa gcgaaaccga aagtagcctg cccggaatgc gatattccgg
    53641 tgatttacgg tgatacgcag aaatcgaacg tgctggaaaa taacgatgtt gaagatgtcg
    53701 ctatctcccg cgagcagcag aatgtttctg taccaatgcc gcagctggag caggcaaatc
    53761 aggcgttagt ggcgaagact ggcgcgcagg agtacgcacc gcacgtcatc aacactccgc
    53821 tggcattcct gattaagagt gctttgaaca ccaatatctt tggtgaacca ggctggcagg
    53881 gtaccggctg gcgtgcaggt cgtgatttgc agcgtcgcga tatcggcggg aaaaccggta
    53941 ccactaacag ttcgaaagat gcgtggttct cgggttacgg tccgggcgtt gtgacctcgg
    54001 tctggattgg ctttgatgat caccgtcgta atctcggtca tacaacggct tccggagcga
    54061 ttaaagatca gatctcaggt tacgaaggcg gtgccaagag tgcccagcct gcatgggacg
    54121 cttatatgaa agccgttctt gaaggtgtgc cggagcagcc gctgacgccg cctccgggta
    54181 ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc taatggtggc aacagccgcg
    54241 aagagtattt catcgaaggt acgcagccga cacaacaggc ggtgcacgag gtgggaacga
    54301 ccattatcga taatggtgag gcacaggaat tgttctgatt aaaaaggcgc ttcggcgcct
    54361 tttcagttgg ctgacaaagt gcatttgttt atgccggatg cgggtgaacg cgttatccgg
    54421 ctaacaaaat cgtgaaaaat caatatattg caggaaaccc ataagcctga taagcgttgt
    54481 gcatcaggca aacttcacgc atttacactc gcccctgccc tttcaaccat tcgcgcacga
    54541 ggaacagcgc gctgacatta cgcgcttcgt tgaagtcagg gtcttccagc aaatccatca
    54601 tatgcgccag cggccagcgc acctgtggta gcggctctgg ctcatcgcct tccagcgatt
    54661 ccgggtagag atcttgcgcg accacgatat tcattttgct ggaaaagtaa gacggtgcca
    54721 tgctgagctt cttcaaaaaa gtcagatcgt tcgcaccaaa tccaacctct tcttttagct
    54781 cgcggttagc ggcttcgtag acgctttcac ccggatcaat taatcctttc gaaaaaccta
    54841 attcgtagga ttcagttccc actgcgtatt cgcggatcag gatcaggtga tcgtccacaa
    54901 tcggcacaat catcactgct tcccggttgg ttggacgcat tcgttcataa acacgccgca
    54961 cgccattgct gaactccaga tccacgctct cgacggtaaa cagtcgggaa cgggctacag
    55021 tttcaacatt cagaatggtg ggtttttgta atgatttgct catcgtggga tctatgctgt
    55081 gaaatcagcg gttattgtgc gatatcggac acgctttcgg caatgtgaat tgcatgttat
    55141 ttacatttat gtaacttaat aaataatcgt cctcaaatca aattaaaagt caataggttg
    55201 aaataactcc aggaatttgc tgatattccg ccttcagagg gtttgctatg atcagcggtt
    55261 actgtgatgt gcttaatgat gctcaagtta aactccacgc ttgccgatag ccaaccgcag
    55321 aatcatgtat tgtgtctggt gcgactgacc acgcctgaca gactaagtaa gatggggaaa
    55381 gcatgagcac cattgtgatt tttttagctg ctttgctggc ctgctcacta cttgcgggat
    55441 ggctgataaa agtgcgatcc agacggcgtc agctgccctg gaccaacgcc ttcgcggatg
    55501 cgcaaacgcg taaactcaca cctgaagaac gtagcgccgt tgaaaattat cttgagagcc
    55561 tgacgcaggt attacaggtg cctggcccaa cgggagccag cgcggcaccg atctctctgg
    55621 cgctgaatgc cgaaagcaac aacgtcatga tgctgacaca cgctatcacg cgttacggca
    55681 tctctaccga cgatccgaat aaatggcgtt actacctcga ttcggtagaa gtccacctgc
    55741 cccctttctg ggaacaatac atcaacgatg agaataccgt tgaactgatt tataccgatt
    55801 cgctgccgct ggttatttca ctcaacggtc atacgctgca ggagtacatg caggaaacgc
    55861 gcggctatgc cttgcaacct gttccgtcca cgcaggcgtc gattcgcggg gaagagagtg
    55921 agcaaatcga gctactcaat attcgcaaag aaacgcatga agaatatgcg ctgagtcgtc
    55981 cgcgcgggct gcgtgaagcg ttgctgatcg tcgcctcctt cctgatgttc tttttctgcc
    56041 tgattacccc ggatgtattt gttccgtggc tggcaggcgg cgcattactg ctgctgggcg
    56101 caggtctgtg ggggctattc gcgcccccgg caaaatcctc cctgcgagaa attcattgtc
    56161 tgcgcggtac accccgtcgt tggggattgt ttggcgaaaa cgatcaggaa cagatcaaca
    56221 atatttcact cggtattatc gacctggtct atcccgcaca ctggcagcca tacattgctc
    56281 aggatctcgg tcaacaaacc gatattgata tctatctcga ccgccacgta gtgcgtcagg
    56341 ggcgatatct ttcgctgcat gatgaagtaa aaaactttcc gttacagcac tggctgcgca
    56401 gtacgattat cgctgcgggc tcgctgctgg tgctgtttat gctgttattc tggatcccgc
    56461 tggatatgcc gctgaaattc actctctcat ggatgaaagg cgcgcagacc attgaagcca
    56521 ccagcgtaaa acaactggct gacgctggcg tgcgggtagg cgatacattg cgtattagcg
    56581 gtacgggaat gtgtaatatt cgcacttccg gaacctggag cgcgaaaacc aattcacctt
    56641 ttttaccgtt tgactgctcg cagatcatct ggaatgacgc ccgctcatta ccattaccag
    56701 aatctgaact ggtcaacaaa gcgacggcat tgactgaagc agttaatcgc cagctgcacc
    56761 ctaaaccgga agatgaatct cgcgtcagtg cctcattacg ttcagcgatt caaaaatccg
    56821 gcatggtatt gcttgatgat tttggcgaca ttgtactgaa gacagcggat ttatgttctg
    56881 ccaaagatga ctgtgtgcga ctgaaaaatg cgctggtcaa tctcggcaac agtaaagact
    56941 gggacgcgct ggtaaaacgc gccaacgccg ggaagctcga tggcgtgaat gtgttattac
    57001 gcccggtgag tgcagaatcg ctggataacc tggtggcaac ctccaccgcg ccgtttatca
    57061 cgcatgaaac ggcgcgagcg gcacaatcac taaacagtcc ggcccccggc ggattcctga
    57121 ttgtcagcga cgaaggcagc gattttgttg atcagccctg gccttcggca tcactttacg
    57181 actacccgcc gcaagaacag tggaacgctt tccagaaact ggcacaaatg ctgatgcata
    57241 cgccgtttaa cgccgaaggt atcgtcacaa aaatcttcac tgacgccaat ggtacgcagc
    57301 atattggcct tcatccgatc ccggatcgtt ccggcctgtg gcgctatctc agcaccacat
    57361 tgctgctact gacgatgctg ggtagcgcca tttacaatgg cgtacaggcc tggcgtcgtt
    57421 accagcgtca tcgcactcgc atgatggaga ttcaggccta ttatgaaagc tgcctgaacc
    57481 cgcaactgat caccccttca gaaagcctta tcgaataaca cgtttgcgcg gcaggttatg
    57541 ctaccctgtc gcgcaaattg cttcactctg gagatttccc tcatgcatat caacattgcc
    57601 tggcaggacg tagataccgt tctgctggat atggacggca cgttgctcga cctcgccttc
    57661 gataactatt tctggcaaaa gctggtgcct gaaacatggg gcgccaaaaa cggggttacg
    57721 ccacaggaag cgatggaata tatgcgccag caatatcacg acgtacagca tacgctaaac
    57781 tggtactgtc ttgattactg gagtgagcaa ctgggtctgg atatctgtgc gatgaccacc
    57841 gagatgggac cgcgtgccgt actgcgtgaa gataccattc cgtttcttga ggcactgaaa
    57901 gccagcggta agcagcgaat tttgctcacc aatgcgcatc cgcacaacct ggcggtaaaa
    57961 cttgagcata ccggtctgga cgcacacctt gatttattac tttccaccca cacatttggt
    58021 tatccgaaag aggatcagcg gttatggcat gcggtggccg aagctacggg tctgaaagct
    58081 gaaagaacgc tgtttattga tgacagcgaa gcgattctcg atgctgccgc gcaatttggt
    58141 attcgttact gcctcggcgt gactaatcct gattccggga ttgccgagaa acagtatcaa
    58201 cgccatccgt cactgaatga ctaccgccgc ctgatcccct cgctaatgtg aaggagacgc
    58261 catgaaagag aaacctgctg ttgaggttcg actggataaa tggctatggg ctgcccgttt
    58321 ttataaaacc cgcgcgctgg cccgtgaaat gattgaaggc ggtaaggtgc attacaacgg
    58381 gcagcgcagc aagccgagca aaatcgtcga gctgaatgcc acgctcactc tgcgccaggg
    58441 aaatgacgaa cgcacggtga ttgtaaaggc gattactgaa cagcgtcgcc ccgccagcga
    58501 ggcagccttg ctgtatgaag agactgcgga aagtgtagag aaacgcgaaa aaatggcgat
    58561 ggcacgtaaa cttaatgcct taaccatgcc gcacccggac cgacgcccgg acaaaaaaga
    58621 gcgccgcgac ctgttacgat ttaaacacgg cgacagtgaa taactgtcac ctgcaagaga
    58681 gatgattatg ccgcaacatg accaattaca tcgctatctg tttgaaaatt ttgccgttcg
    58741 cggcgaactg gtaaccgttt cggaaaccct gcaacagatc cttgagaacc acgattatcc
    58801 gcagcccgtt aaaaacgtgc tggcagaact gctggttgcg accagcctgt taaccgctac
    58861 gctgaagttt gatggtgata tcaccgtaca gctgcaaggc gacggtccga tgaatctggc
    58921 ggttattaac ggtaacaata accagcaaat gcgcggtgtg gcgcgcgtgc agggcgaaat
    58981 tccagaaaat gccgacctga aaacgctggt cggcaatggt tacgtggtga tcaccattac
    59041 cccgagcgaa ggcgaacgct atcagggcgt ggttggtctg gaaggtgata ccctggcggc
    59101 ctgcctggaa gattacttta tgcgttctga acagctgccg acgcgcctgt ttattcgcac
    59161 cggcgacgta gacggcaaac cggctgcagg cggtatgttg ttgcaggtaa tgcctgcgca
    59221 aaatgcccag caggacgact ttgaccacct ggcgacgcta accgaaacca tcaaaaccga
    59281 agaactgctg accttaccgg caaacgaagt gttgtggcgt ttgtatcacg aagaagaggt
    59341 gacggtttac gatccgcagg atgtggagtt caaatgcacc tgctcgcgtg aacgttgcgc
    59401 cgatgcgctg aaaacgctgc ctgatgaaga agttgatagc atcctgacgg aagatggcga
    59461 aattgacatg cattgtgatt actgcggtaa ccactatctg ttcaatgcga tggatattgc
    59521 tgaaatccgc aacaacgcgt ctccggcaga tccgcaagtt cattaatgag tatgtccggc
    59581 agagagggct ctgccggatt ttacgacgta atggcctgat gtgctgcgct taccaggcct
    59641 gtgaaataaa tcgcaaatta ttgttggtca gatacagcgt cgtatccgtt aaaagagttg
    59701 tcacttactt aagtttattt ccagcgtttc accatctacc gtcagatcgc gccacgcttt
    59761 cggcctcgtc cccagttttc catccttcgc agctgtgagt aatttttctt tcgttatttc
    59821 gttcggaagt aaactcatat caagcgcgtc ccattctttt ctgtctgagt caaagccata
    59881 gacaatgact ctgtcatcat taaaagcaaa caggatccgc tccgcctgac catcactatt
    59941 cagatcttgc tcgaccagga cgcaggcgtc tttttcaata caggtcatca cgttatagcg
    60001 atcctggatt aaggccgacc agaatgtcgc atccggttta acagaaccag gggcaattaa
    60061 cacattttcg gctaatactt tttccgatac ctgttcctga agatgttgct ctccatctaa
    60121 cgccatcagc agatcccgcg cgcgtttcgg gtctttcata aacccggcat cgcttttcag
    60181 cgactcaagc gccgcacgtc catagcgacc gctctgttcg agcatgtaga tagttacctg
    60241 gtctggcgtg tttttgccgc tctgataacg cgccatatga ctgttcacgc taatacgcat
    60301 actatccagc accggcgaat taagaagcac cagtatcacc aacaccaata acgaaaccgc
    60361 aaggttcact ttgccctgaa gaacaagggg attttgccca ttacgccaga cgatactgac
    60421 aaaatacccc agcgaccaca ccagtaacac cagcaccgcc agcgcgccct gcaagcggtc
    60481 gacagtccag ccgtactgag cgacccgcag ccataacgcc caggcggcga cgaacacata
    60541 cagcggagca accagcaaag cggttttaat caggcaacgc aacggccccg tccagggaag
    60601 tgacgctttt tgcggatcgc ggacaatagc cattaagatc aattgcaaaa aggccagcgt
    60661 taacagcaac ccggcggcgg agatgtggcg agaaatcgcg ctcaggcccg taaacggcag
    60721 agtgatgata aacatcaggg ttagcaatga tactaacggc agcaaccccg tggcgattaa
    60781 cgtgaacaac ttttgaatag agtcgattaa acgtgactgt gtgcgcgcga gaatcaccgc
    60841 cagtgcggta accagaccta atgtaagata cataaaccag tcggttgcaa aaaagagcgt
    60901 attgaaaaac ttgatgccaa caagtttaaa caactcaccc cacagtaaaa gcaccaccca
    60961 cgtcaggcca ttggcgagaa aaatcaccag taatataagt acgttatgcc atactgactt
    61021 gtaaaagtag ctatagcggc tactgccatt gcggatacga aggctttgtt gtatccacgg
    61081 tagcaacaac attgccatca acagcagata gcagccaaaa tcccaaatcg ccttttcagc
    61141 tctccagggg ttcatgccat cagtttgcca cttcagccaa ccgctcatcc ctgtcgtggc
    61201 aataaacacc agcgccagcc atccccaaag gcgcttctgt ttaaaagaga tcacggagaa
    61261 gagtaaaacg gatgaaaacg caacggtcgc aggcacgcca taaacaatcc aactgtgatt
    61321 ttttccggca agccaggcga tcaacaggta gcaaaccagc ccctgaagta atccggtagc
    61381 aatcatcccc cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa
    61441 tcaaaaggtg ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa
    61501 tcaaaaaaag ttagcgtggt gaatcgatac tttaccgatt gaatttgcat caatttcatt
    61561 aaggaatgcg attccactca caatattccc gccatataaa ccaagattta accttttgag
    61621 aacattttcc acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa
    61681 tcacggagtt tttgtcaaat atgaatttct ccagatacgt aaatctatga gccttgtcgc
    61741 ggttaacacc cccaaaaaga ctttactatt caggcaatac atattggcta aggagcagtg
    61801 aaatgcgcgt taacaatggt ttgaccccgc aagaactcga ggcttatggt atcagtgacg
    61861 tacatgatat cgtttacaac ccaagctacg acctgctgta tcaggaagag ctcgatccga
    61921 gcctgacagg ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc gtcgataccg
    61981 ggatcttcac cggtcgttca ccaaaagata agtatatcgt ccgtgacgat accactcgcg
    62041 atactttctg gtgggcagac aaaggcaaag gtaagaacga caacaaacct ctctctccgg
    62101 aaacctggca gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa cgtctgttcg
    62161 ttgtcgacgc tttctgtggt gcgaacccgg atactcgtct ttccgtccgt ttcatcaccg
    62221 aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc gatgaagagc
    62281 tggcaggttt caaaccagac tttatcgtta tgaacggcgc gaagtgcact aacccgcagt
    62341 ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt taacctgacc gagcgcatgc
    62401 agctgattgg cggcacctgg tacggcggcg aaatgaagaa agggatgttc tcgatgatga
    62461 actacctgct gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac gttggtgaga
    62521 aaggcgatgt tgcggtgttc ttcggccttt ccggcaccgg taaaaccacc ctttccaccg
    62581 acccgaaacg tcgcctgatt ggtgatgacg agcacggctg ggacgatgac ggcgtgttta
    62641 acttcgaagg cggctgctac gcaaaaacta tcaagctgtc gaaagaagcg gaacctgaaa
    62701 tctacaacgc tatccgtcgc gatgcgctgc tggaaaacgt caccgtgcgt gaagatggca
    62761 ctatcgactt tgatgatggt tcaaaaaccg agaacacccg cgtttcttat ccgatctatc
    62821 acatcgataa cattgttaag ccggtttcca aagcgggcca cgcgactaag gttatcttcc
    62881 tgactgctga tgctttcggc gtgttgccgc cggtttctcg cctgactgcc gatcaaaccc
    62941 agtatcactt cctctctggc ttcaccgcca aactggccgg tactgagcgt ggcatcaccg
    63001 aaccgacgcc aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg cacccgactc
    63061 agtacgcaga agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct tatctggtta
    63121 acactggctg gaacggcact ggcaaacgta tctcgattaa agatacccgc gccattatcg
    63181 acgccatcct caacggttcg ctggataatg cagaaacctt cactctgccg atgtttaacc
    63241 tggcgatccc aaccgaactg ccgggcgtag acacgaagat tctcgatccg cgtaacacct
    63301 acgcttctcc ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg tttatcgaca
    63361 acttcgataa atacaccgac acccctgcgg gtgccgcgct ggtagcggct ggtccgaaac
    63421 tgtaatgatt tgaagctgga gaatatctat ccagtatctt atagaaagca aaacgggagg
    63481 caccttcgcc tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc
    63541 actggcagcc aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag ctccagcatc
    63601 ccgttatggt tatccacgat acgctgcaca attgccagcc ctaatcccgt gccgctaatg
    63661 gtgcgcgcac tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg ttccggcgca
    63721 attcccggac cgtcatcttc cacctggaac caggcgcgat tcggctccgt tccgctgctg
    63781 actttgatcc agccattgcc ataacgggcg gcgttgacca ccatattcgc caccgcgcgt
    63841 ttgatcgaca gcgggtgcat tttcacttca atgctgccgg ggtaaagcgc ggtttcaatt
    63901 tcccgctcat agccactttc ggcagcaatc acctcaccga gtactgcatt aagatccgcc
    63961 atttccatcg gcatctcctg cccggtgcgc aggtagtcga taaactgctc aatgatggcg
    64021 ttgcactctt cgatatcttt attgatcgat tctgccagat agccatcctg ctcgctcatc
    64081 atctcagtcg ccaggcgaat acgcgtcagc ggcgtgcgca agtcgtgact tacccccgcc
    64141 atcagcagcg tgcggtcatc cgccagttgc ttaacaccag ccgccatatg gttaaaggca
    64201 cgggtaacgg aacgcacctc ggacgcgcca tactcacgca gcggcggcgg aataatccct
    64261 ttaccaacct gcaaggctgc gtgttcgaga tcgaccaacg gtcggttctg gatacgaata
    64321 aacagccacg ccccgcctat cgccaatagc ataatcgcca gcgtatagcg gaacagcgga
    64381 gagaaatcgc cctgatgaat ttcggtcagc ggcacgcgta cccagatatt gggcgacagc
    64441 caggttttca gccagacgac aggcgaactt ttgttgacct caacgcgcac ttccgtcggg
    64501 ccgcccagtt gctgtgccat ctgatggctt aagaattcat agtgttgcgc ccaacgcaga
    64561 cctgcctctt cggcagcctc gttggagtag agagagatcc ccagctcacg gtagatctcc
    64621 cgacggaaag cgggaggcac aaccaactgc gtgccgtcct ccagttgcag tttgtcggtc
    64681 atcaacatac ggacttcgta cgcgaggact ttattaaact gctggaggct cggcaaaatc
    64741 gcgaagttca gcaccaccag ataagtcgtc accaggctgg cgaacagcaa ggtgacgatg
    64801 agcaataacg tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct catgctttag
    64861 agccgtccgg tacaaagacg tagcccaggc cccaaacggt ctgaatgtaa cgcggatgcg
    64921 ctggatcttc ttctaccatg cggcgcagac gcgaaatctg cacgtcgatg gagcgttcca
    64981 ttgcggaata ttcacgacca cgggcaaggt tcatcagctt atcgcgggag agcggctcac
    65041 gcggatggct gaccagtgcc ttcagtaccg caaactcacc gctggtgagc ggcatcggct
    65101 catcttcgcg gaacatttcg cgcgtaccga ggttaagttt gaacttaccg aaagcaatta
    65161 ccgcctcttc ctgtgacggt gcgcctggca gttcgttcgc ctgacgacgc agcaccgcac
    65221 gaatacgggc cagcagttca cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa
    65281 tctccaggcc tacgatacgg tccacttctt cccctttcgc cgtcaccata atgatcggca
    65341 tcgggttgct ctgactacga agacgtcggc aaatcgacaa gccatcttca ccaggtaaca
    65401 ttaaatccag taccataaga tggaaagatt cacgagtcag caggcgatcc atctgttctg
    65461 cattagcgac gcttcgaacc tggaagcctt gttcggtgag ataacgttcc agcagcgcac
    65521 gcaggcgcat gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt gtttgtactc
    65581 ccaaaggttc gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag
    65641 ctaaatctgg tatgaatttc agcctaaatt gttacaaagc atattaaaca gcagcttaag
    65701 tatacaattt attcagcgaa acattattga ttctgttgat atgatcacgt tatacccaat
    65761 gtgcgcatta tcaaacagac aaagggaatc aacgagatga aaacgcccct ggttacccgg
    65821 gaagggtatg aaaaactcaa acaagagctt aattatctct ggcgtgaaga acgcccggag
    65881 gtcacaaaaa aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat
    65941 cagtataata aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc
    66001 atggaaaatc tcaaaatcgt cgattactcc cctcagcagg aaggcaaggt cttttttggc
    66061 gcatgggtgg agattgaaaa cgacgatggc gtgactcacc gtttccgtat tgtcggctac
    66121 gatgaaattt ttggccgtaa agattacatc tctatcgatt ccccgatggc ccgcgcattg
    66181 ctgaaaaaag aagtcggcga tctggcggtg gtgaataccc ctgccgggga agcgagctgg
    66241 tatgtcaatg ctatcgagta cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg
    66301 ctggcatttt tgccagccag tccgtataac tatcccctga tttttgatcc gaaaagatga
    66361 actcaaacca tgatgaatga ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc
    66421 ccggaacagg ttgacgctgc cgttcgcctg cttgacgaag ggaataccgt gccgtttatc
    66481 gcacgttatc gtaaggaaat caccggcggt ctggatgaca cgcagctgcg taatctggaa
    66541 acgcgtctga gctatctgcg cgagctggaa gagagacgtc aggcgatcct caaatccatt
    66601 tccgagcaag gcaaactcac cgatgatctg gcgaaggcca tcaacgccac actaagcaaa
    66661 accgaactcg aagatctcta cctgccctac aaacctaaac gccgcacccg cgggcaaatc
    66721 gccattgaag cagggcttga gccgttggct gacctgctgt ggagcgatcc gtcacacacg
    66781 ccagaagtcg ccgctgcaca atatgttgat gcagataaag gcgtggcaga taccaaagcc
    66841 gcgctggacg gcgcgcgcta tatcctgatg gaacggtttg ccgaagatgc cgcgctgctg
    66901 gcgaaagtgc gtgattatct gtggaagaac gcgcatctgg tctcaaccgt gatgagcggt
    66961 aaagaagagg aaggggcaaa attccgcgac tatttcgatc atcacgaacc gctgtccacg
    67021 gtgccttctc accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt actccagctt
    67081 tcgctgaatg ccgatccgca gttcgaagag ccgccaaaag agagctattg cgagcaaatc
    67141 atcatggatc atctcggcct gcgcctgaac aatgctccgg cggatagctg gcgcaaaggc
    67201 gtggtgagct ggacctggcg catcaaggtg ctgatgcatc tggaaaccga actgatgggc
    67261 accgtgcgcg aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa cctgcacgat
    67321 ctgctgatgg cggcccctgc cggactgcgt gccacgatgg gcctcgatcc gggtctgcgt
    67381 accggggtaa aagtagcggt ggtcgatgcc actggcaaac tggtggcgac cgataccatt
    67441 tacccgcaca ccggacaaac agcaaaagca gcgatgaccg ttgctgcctt gtgtgaaaag
    67501 cataacgttg aactggtggc gatcggcaac ggtacagctt cccgcgaaac cgaacgtttc
    67561 tatctcgacg tgcagaagca gttcccgaaa gtgaccgcgc agaaagtgat cgtcagcgaa
    67621 gctggcgcgt cggtttactc ggcttccgag ctggcagcgc aggagttccc ggatctcgac
    67681 gtttcgctgc gtggtgcggt atctatcgcc cgccgtttgc aggatccgct ggcggagctg
    67741 gtgaaaatcg atccgaaatc tatcggcgta ggtcagtatc agcatgacgt cagccagacg
    67801 caactggccc gcaaactgga tgcagtagta gaagactgcg taaacgccgt tggcgtcgat
    67861 ctcaacaccg cttctgttcc gctattaacc cgcgtggcgg gcctgacgcg catgatggcg
    67921 caaaacatcg tggcctggcg cgatgagaac ggccagttca agaaccgtca gcaactgctg
    67981 aaagtcagcc gtctggggcc gaaagccttc gagcagtgcg cgggcttcct gcgcattaac
    68041 cacggtgata acccgctgga cgcctctacc gttcacccgg aagcctatcc ggtggtggaa
    68101 cgcattctgg cagcaacaca gcaggcactg aaagatctga tgggtaacag cagcgaactg
    68161 cgtaacctga aagcgtctga ctttactgat gaaaaattcg gtgtgccgac agtaactgac
    68221 atcatcaaag agctggaaaa accgggtcgc gatccgcgtc cggaatttaa aaccgctcag
    68281 tttgccgatg gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct cgaaggcgct
    68341 gtcaccaacg tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg
    68401 gttcacatct cttcattgtc gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg
    68461 ggcgacattg tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg tatcgccctg
    68521 accatgcgtc tggacgagca gcctggcgaa accaacgccc gtcgcggcgg cggtaatgaa
    68581 cgcccgcaaa acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca gcctgccggt
    68641 aatagcgcga tgatggatgc gctggcggcg gcaatgggca aaaaacgtta aacgccctga
    68701 ttgaccccta aacctgcgcg ataaggtgtc cacgccgcat ctggcaacga acgccttgtc
    68761 tgacataaaa gtgccggaga atatctccgg catttttatt ccacagccaa actcacaata
    68821 tattccggca acatttatca cttcattaac aactgaaacc ttaattaaac attagccagt
    68881 cctggtaatt cgctattcga attatatttt cgctgcgata taaccttgag ccacatcaac
    68941 attgagtcag attattattc aaaccaacat tcgcacacat tttaagtatt gctgatagaa
    69001 accattctca ttatcattgt gctgttgatt atttaatctc tccttcgttg gcaaatcatc
    69061 tggtctcatg tcgctgtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt
    69121 aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt tcccgtgaaa
    69181 tcagcccggc atatcgccaa aaactgcttt ctcttggcat gttacctggc tcctctttta
    69241 atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc
    69301 tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg
    69361 gtgataacaa caatgaaaaa attaaccata ggcttaattg gtaatccaaa ttctggcaag
    69421 acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg ggctggcgtt
    69481 accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac
    69541 ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc
    69601 gcctgtcact acattttgag tggcgacgcc gacctgctga ttaacgtggt ggatgcgtct
    69661 aaccttgagc gtaacctgta cctgacgcta caactgctgg aactcggcat tccctgcatc
    69721 gtggcactga acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgct
    69781 ctgtcggcgc gtcttggctg cccagtgatc ccgctggttt caacccgtgg tcgcggtatt
    69841 gaagcgctca agctggcgat tgatcgctat aaagctaacg agaatgtgga actggtgcat
    69901 tacgcacagc cgctactcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc
    69961 ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc
    70021 gcctacgccg gtgaagcgtc gcagcatctg gatgccgccc tcgcccgtct gcgtaatgag
    70081 atggacgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt
    70141 gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa
    70201 atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc
    70261 ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg ctccgtggcg
    70321 ctatttgtgc atggtattca atggattggc tacacgctcc acttcccgga ctggctgact
    70381 atcttcctcg cccagggcct gggtggcggc attaacaccg tgctgccact ggtgccgcag
    70441 attggcatga tgtacctgtt cctctccttc cttgaggact ccgggtatat ggcgcgtgcg
    70501 gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc cggggaaatc ctttgtgccg
    70561 ctgatcgtcg gtttcggttg taacgtaccg tcggtaatgg gtgcacgtac gcttgatgca
    70621 ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg
    70681 gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgcgct ggcggtcttc
    70741 tcgctgtata tgctgggtat tgtgatggcg gtgctgactg gcctgatgct caagtacacc
    70801 atcatgcgcg gtgaagcgac gccgtttgtc atggagttgc cggtctatca tgtaccacac
    70861 gttaaaagcc tgattatcca gacctggcag cgtctgaaag gcttcgttct gcgtgctggt
    70921 aaagtgatca tcatcgtcag cattttcctg agcgctttca acagcttctc gctgagcggg
    70981 aaaatcgtcg ataacatcaa cgactcggcg ctggcgtccg tcagccgggt gatcaccccg
    71041 gtcttcaagc caattggcgt gcatgaagat aactggcagg caacggttgg cctgtttaca
    71101 ggtgccatgg cgaaagaagt ggtagtgggt acgctcaaca ccctctacac cgcagaaaat
    71161 attcaggacg aagagttcaa tccggcagag tttaacctcg gtgaagagct gttcagtgcg
    71221 atagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc
    71281 attgaagcca gcaaaggcga cggcgaaatg ggtaccgggg cgatgggcgt gatggatcag
    71341 aaattcggta gcgcagcagc agcttacagc tacctgattt tcgtcctgct gtatgtacca
    71401 tgtatctcgg tgatgggagc catcgcccgt gaatcaagcc gtggctggat gggcttctcc
    71461 atcctgtggg ggctgaatat cgcttactcg ctggcaacat tgttctatca ggtcgccagc
    71521 tacagtcagc acccaactta cagcctggtg tgcattctgg cggttatcct gtttaacatc
    71581 gtggttatcg gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact gctggcaacc
    71641 cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt
    71701 cacttattca ggtgcgcgat ttgctggcgt tacggggccg tatggaagcg gcccagataa
    71761 gccagacatt gaacactcca cagccaatga ttaacgccat gctgcaacaa ctggaaagta
    71821 tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc agttgtaaaa
    71881 gctgcccgga aggaaaagcc tgtctgcgcg agtggtgggc gctgcgttaa ccttactcca
    71941 tcgcctgttt tggaaagccc ggtatgcgtc tgcatccggg cttttttgcg tgcggctttc
    72001 cataaaaatg caactcttgc agcacggcgt aagttccttt gaaagcatct cgcagggatg
    72061 aaaactcgct aatacacagg tgtggagtgg cgcgtagagt cgcggcattc aaacaacagg
    72121 tgaaggaacg ccatgagcaa aaagcagagt tccaccccac acgatgcgct gttcaaactc
    72181 tttttacgcc aaccggacac ggctcgtgat tttcttgcgt ttcatttacc ggcacccatt
    72241 cacgcgcttt gtgatatgaa aaccctcaag ctggagtcga gcagctttat tgatgacgat
    72301 ctgcgtgaaa gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg accaggatac
    72361 atctattgtc tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg
    72421 cgttacgcta ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac gttgccgatg
    72481 gtggtgccat tgttgtttta ccacggtatt gaaagcccct atccctattc gctgtgttgg
    72541 ctggattgtt tcgccgatcc caaactggca aggcagcttt atgcctccgc atttccgctg
    72601 attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat ggcgctgctg
    72661 gagttaattc aaaaacatat tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc
    72721 tgcttattaa gtagtggata cgctaatgac agacaaatca aagggctgtt taattacata
    72781 ctgcaaaccg gcgacgcggt acgttttaac gattttatcg acggcgttgc cgaacgttca
    72841 ccgaaacaca aggagagttt aatgacaatt gcggaaagat tgcggcagga gggggaacaa
    72901 tccaaagccc tgcatatagc caaaataatg cttgaatccg gagtccctct tacagacatc
    72961 atgcgcttta ccggggtgtc agaagaagag ttggctgcgg cgagccagta aatttctgtc
    73021 tcgccatttc aaaagttgcc tacaccctct gcttcaacgc caccagcagg tgacaaaact
    73081 cgaccggatg cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt
    73141 gaggccaaag tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc
    73201 catacaatcg caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt
    73261 tcaggatttc cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg
    73321 ttttcttcaa cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca
    73381 ggaaccgctc cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca
    73441 cgtccggttt tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg
    73501 tgaccagcgc ctgaacacgc tcgggatggg ttaaaacaat ttggcttgcc accagcccgc
    73561 ccagactcca gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca
    73621 tatcagcaag tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa
    73681 ggtgcagcgt aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca
    73741 gtccccatcc gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga
    73801 tgttattcat ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac
    73861 cgggattatg ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg
    73921 tctgttcacg cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca
    73981 cacactccca tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg
    74041 tcacggttgc cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc
    74101 ggcgcagtga aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc
    74161 gtcgcaccac cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc
    74221 gtcactggcg tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt
    74281 tgcattgcca atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt
    74341 ttctcagtgc ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg
    74401 tgcaaggtcg ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag
    74461 agattgcgca gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc
    74521 gaaccttgta gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag
    74581 gccattacta tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca
    74641 aatcaggaag aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct
    74701 gaatgtggtg tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa
    74761 tttgacctgc tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag
    74821 atcgactttg ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa
    74881 atgcgtaaag tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg
    74941 cagatcaacc cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa
    75001 gacggttacg ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg
    75061 acgctgaaag aagggatcga gaagcaactg ctgaacgaat tcccggagct gaaaggtgtg
    75121 cgcgatctca ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt
    75181 gtagattgcc cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga
    75241 cgttggcaca gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca
    75301 acctctcata atttaaattt accccgctct ggtgattctc aaacgccaga tgttacccgt
    75361 atcattcaca tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg
    75421 ggtatcagtc gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt
    75481 cagaaaattg acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt
    75541 gttgctatcg gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc
    75601 atcgctctcg tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa
    75661 gttattggct ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg
    75721 ggttttggcg caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc
    75781 accacgctga ttgccaaatt tggtaaaaaa cacatccagt gggcggtggt actgaccggt
    75841 tttaccgttg gttttgccct gttctatgaa gtgggctttg tgctgatgct gccgctggtg
    75901 tttaccatcg cggcttctgc gaatattcca ctgttgtatg ttggtgtacc aatggcagct
    75961 gcactgtctg tgacccacgg cttcctgcca ccgcatccgg gtccgactgc gattgccacc
    76021 attttcaatg ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc
    76081 gtgattctcg ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaattccg
    76141 gaaggtctct acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc
    76201 gtctggacct ctctggtgcc ggtagtactg atggcgatgc gtgcgattgc cgaaatgatc
    76261 ctgccgaaag gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggct
    76321 acgctgattg ccgtgctgat tgccatgttc acctttggtc tgaaccgtgg tcgttcaatg
    76381 gatcagatta acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc
    76441 atcggtggtg gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt
    76501 gcttccatga tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc
    76561 gtactgcgta tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg
    76621 gcaccgctga ttgcaacgac gggtgttagc ccggagctga tggttattgc ggtgggttcc
    76681 ggtagtgtga ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt
    76741 aacctgacta tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg
    76801 tgcggtctgg taggctgtct gctgctgaat atggtgattt gataacactg actgccggat
    76861 gcatcgcatc cggcatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg
    76921 tccaaatcct tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg
    76981 gaaagcttgc gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg
    77041 gccatatcca gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg
    77101 tagcgctgca aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg
    77161 gcacgttttg gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt
    77221 tcgcgatcct gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg
    77281 cccagcgtta gatccccgct ctcccagtaa ccgcgtagcg taggcaggtc atgtgtcgcc
    77341 gcaaccgcca tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg
    77401 ttttcgaaat agagcacttt gtaggagtac acaccgctgc tgcgcagctt accgacaatc
    77461 tctaccggta cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg
    77521 agtgccagaa tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc
    77581 gtctcgccat acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat
    77641 gcgccgcagt tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg
    77701 gtgatgatat gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata
    77761 tccggcggcg cgccaaccga cgctttcagg caatatagtt cacggtcaca ccaggtttcc
    77821 gccccacctt ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag
    77881 ccctggctta tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga
    77941 taaaaatcga cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg
    78001 ttctgataca tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc
    78061 tgggcatgta gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg ctctgcaaca
    78121 aactggcgaa acgcggtcat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt
    78181 cgtaatgctg tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc
    78241 gcctgttgca gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta
    78301 agatggaaat cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa
    78361 gacgggctgt atgggctggc actctccgga tttgccggat agagcgcatg aatcgggttc
    78421 aggccgatga acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca
    78481 aaatccccaa taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc
    78541 cacagctttt gtttattcag caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc
    78601 acccggcaat gcgcgcgctg gtcgtcctgg gtgagtgtca gcgtgtgata accttccggc
    78661 agcttcgtcg gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgtgttcct
    78721 tcttcggtgg tcagcagcca gctatattcg ccgctgccct ccaccaccat cggcattttt
    78781 ttgccgctgg tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta
    78841 cgttgatgca tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta
    78901 ccgtgggcat tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt
    78961 ttgctttcca tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga
    79021 atagagcgat ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc
    79081 caggcctcct ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg
    79141 aaatccgcca tcaccagata cggatcgccg ccttgtttgc cgatactgtg cagcatctgg
    79201 tcgaaggcat gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg
    79261 tccagcacct tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc
    79321 ttcacttgtt ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg
    79381 atttcaacgt tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc
    79441 atattgccgg taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc
    79501 gccgggatca gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac
    79561 ttatcgccaa ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata
    79621 atattcttcg ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt
    79681 acgcgatcag cctgcgggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga
    79741 ttcaggtgct ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt
    79801 ggattgatct caataccggt acgaactttc acaaactccg ccagacggac tttattcgcc
    79861 tgcttgatct cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg
    79921 atcagctgat cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc
    79981 agtgcagggt tgcactgttt gatccagcga cgtggggtaa taccgttggt gacgttatgg
    80041 aatttgttcg gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag
    80101 tgcagcgccg caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc
    80161 acttgtttat cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt
    80221 ttctccacca gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc
    80281 agtaagcctt tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg
    80341 ttggtgtaag cgaaagtttt gctggtaatg gcccaggcgt catcccagct catctggtgc
    80401 tcatcgatca gcacgcgcag cagttctgga atcgcgatag ttgggtgggt atcgttcagc
    80461 tgaataactt cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc
    80521 aaaatatccg ctaccgaaca ggcacactgg aagtactgct gcatcaagcg cagcttttta
    80581 ccggcagtat ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgccc
    80641 tgctgttcgg cacgcaggaa atcaccgtcg ttaaatttag tcagatcaaa cggatgcgcg
    80701 tgcgtcgcct gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg
    80761 agatcccacg cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcgtcact
    80821 ttaccgccaa tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta
    80881 ctgcgatgcc agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac
    80941 aaaccatatt gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag
    81001 tcgaggaagc acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct
    81061 tcttccagca ggtccgtcag attgatgtca taagccttca acgaatcctg tacatcctga
    81121 taccagccga gattcaacag gttgttgccc gtcaggcgac caatcaaaaa ctccattgag
    81181 atgtagttaa catgtcgctg attcgccacc ggcttggcga atggctgagc acgcagcatt
    81241 tcggccagtg cttcactcac tgctagccac cactggcgag gagtcatttc agccgcagaa
    81301 tttaagccat aacgctgcca ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa
    81361 ataggttgtg acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt
    81421 ttgccaggct cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg
    81481 gggatgagca aggaaatgtg atctcaacca cttaaagcta gtgcaaacca caggattagc
    81541 agcaaatcaa tgcaatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc
    81601 gtattttcaa aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcat
    81661 aagtaaaata tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt
    81721 agatggcact ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat
    81781 aagggaaacc agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt
    81841 tggaattgtg acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa
    81901 aggtttctgg ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc
    81961 attttccaca gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg
    82021 actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt
    82081 ccggctggcg ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg
    82141 ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga
    82201 gcgtttcgcc agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat
    82261 atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct
    82321 tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct
    82381 gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa
    82441 tctcaccctg gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt
    82501 tcgtgatcaa ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa
    82561 gcagtttttt gattgccgtc tgtcatcgcc gattgaagct gcagaaagca gtcggatttg
    82621 cgatgacgtt tccggttggg cgacggcact acagctaatc gccctctccg cccggcagaa
    82681 tacccactca gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc
    82741 ggattatctg gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt
    82801 gaaaagcgcc attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga
    82861 aaacgggcaa atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga
    82921 tgataccggc gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg
    82981 ccagtgggaa ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat
    83041 ggcccaggga tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat
    83101 gctgcgcgat attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct
    83161 gctggaagag tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt
    83221 gttattgcag gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct
    83281 agcccgtgct gaacatgaaa tcaaggacat cagagaaggc accatgcacg cagaatttaa
    83341 cgctctgcgc gcccaggtgg cgattaacga tggtaatccg gatgaagcgg aacggctggc
    83401 aaaactggca ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc
    83461 ggtgctgggt gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctaatgca
    83521 gcaaaccgaa cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat
    83581 ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacacagga
    83641 aaaagcgttc cagctgatca acgagcagca tctggaacag ctgccgatgc atgagtttct
    83701 ggtgcgcatc cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc
    83761 ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct
    83821 ggcaatgttg attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagct
    83881 gaaccgtctg gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc
    83941 caacaaagtc cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg
    84001 gttgcgtcat acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg
    84061 caacattgcc cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga
    84121 agaactcaat gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt
    84181 gctgcttaat caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct
    84241 ggacgcatta aaactggcga atcgcaccgg atttatcagc cattttgtca tcgaaggcga
    84301 agcgatggcg caacaactgc gccagctgat tcaactcaat acgctgccgg aactggaaca
    84361 gcatcgcgca cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca
    84421 tttcgatgag aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactgatccg
    84481 caccagcccg ttgacccaac gtgaatggca ggtcctgggg ctgatttact ccggttacag
    84541 caatgaacaa attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg
    84601 caatctgtac caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa
    84661 actgctgaag atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt
    84721 ttacatcttt acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa
    84781 accgcaccgg caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat
    84841 gggcgacctt aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga
    84901 gatattcccc caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg
    84961 cggctaccac ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat
    85021 tttcactttc gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat
    85081 gaatattctg ttcatgcagg gaaaaactcc ccgccagtgt agcgatttca cgctcagcaa
    85141 catggcgcgg cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct
    85201 cgccaagttg caaggtgtta aacgatgcca cgggcgagac ttccgttgcc accacgccgc
    85261 ctccggcagg ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg
    85321 ccagcagcgg ctccagcacc cggcggataa aatccgcagg cggggccgac gggttatcgg
    85381 tgcctccgct cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg
    85441 tttgcagcac cagcgtacag cttccggcac taccgatagc aaagcggtac tcgccgccgc
    85501 gcacggtgcc aggccggaag accagacgct gcgaccccag ctccgcgcct tccaccgttg
    85561 ccctacaaat ttccgcagcc gcttttaccg cggtcagatg ctggcgcaac agccccggtt
    85621 tcgcccgccc ggcacgaatg ctggtgatgg taaatggctg gccggttatc atcgacaggc
    85681 tcagcgccga gcgcaggatc tgcccgccac cttcgccctg tgcgccatcc agcgcaatca
    85741 tccttttcat catttatcct tttacgcaca ccacctgacg cagggtatag ataacttcca
    85801 ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt
    85861 cgatcacttc ggcatcttta cggcattcca catgcgcggt ggcacgaatt tgatcttcca
    85921 cgctgaacag ttttttcgct ttagtacggc tcattacccg cccggcaccg tggctgcacg
    85981 aacagaacga ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac
    86041 cgggaataat tccatattga ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga
    86101 tctcttcacc aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca
    86161 tcgccagcgt ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca
    86221 cgttttccat catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc
    86281 ttaggtaatc atcaaagtat tccgtacctt ccataaagta cgccaggtcg cgcgatggca
    86341 acgtttcaag ctggtcctgc atctcttttt gtgccaaatc gataaagtaa gtcccgatgg
    86401 cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctggtcc gactcatcaa
    86461 ggcagatttc aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag
    86521 tgttaaggaa acgcggatat ttttgcgtta accactgata accggcttca agctcagccc
    86581 atttcgcatc gacgttaaca ggcgggtttt cccatgcgcc tttatcacgt ttacaacggc
    86641 cagtggtacg tccgtgcggc accgcagttt caatcgcctg acgcagctct gccaggtttt
    86701 caggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca
    86761 cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac
    86821 ctttacccag gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg
    86881 ccgtattaat aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt
    86941 ttaccggggc attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt
    87001 gtttgctgtc gtga
//
LOCUS       DAZUFB010000007        86498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZUFB010000007 DAZUFB010000000
VERSION     DAZUFB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.007"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1013)
                     /locus_tag="ACHY3M_000562"
     CDS             complement(<1..1013)
                     /locus_tag="ACHY3M_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4315538.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRG"
     gene            complement(1472..1558)
                     /locus_tag="ACHY3M_000563"
     tRNA            complement(1472..1558)
                     /locus_tag="ACHY3M_000563"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1522..1524),aa:Leu,seq:gag)
     gene            complement(1573..1905)
                     /gene="secG"
                     /locus_tag="ACHY3M_000564"
     CDS             complement(1573..1905)
                     /gene="secG"
                     /locus_tag="ACHY3M_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4315539.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(2133..3470)
                     /gene="glmM"
                     /locus_tag="ACHY3M_000565"
     CDS             complement(2133..3470)
                     /gene="glmM"
                     /locus_tag="ACHY3M_000565"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4315540.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(3463..4311)
                     /gene="folP"
                     /locus_tag="ACHY3M_000566"
     CDS             complement(3463..4311)
                     /gene="folP"
                     /locus_tag="ACHY3M_000566"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFS4315541.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(4401..6335)
                     /gene="ftsH"
                     /locus_tag="ACHY3M_000567"
     CDS             complement(4401..6335)
                     /gene="ftsH"
                     /locus_tag="ACHY3M_000567"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4315542.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(6435..7064)
                     /gene="rlmE"
                     /locus_tag="ACHY3M_000568"
     CDS             complement(6435..7064)
                     /gene="rlmE"
                     /locus_tag="ACHY3M_000568"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFS4315543.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVNQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            7190..7483
                     /gene="yhbY"
                     /locus_tag="ACHY3M_000569"
     CDS             7190..7483
                     /gene="yhbY"
                     /locus_tag="ACHY3M_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4315544.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7639..8115)
                     /gene="greA"
                     /locus_tag="ACHY3M_000570"
     CDS             complement(7639..8115)
                     /gene="greA"
                     /locus_tag="ACHY3M_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4315545.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDKE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            8363..9796
                     /gene="dacB"
                     /locus_tag="ACHY3M_000571"
     CDS             8363..9796
                     /gene="dacB"
                     /locus_tag="ACHY3M_000571"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS4315546.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9836..11008)
                     /gene="cgtA"
                     /locus_tag="ACHY3M_000572"
     CDS             complement(9836..11008)
                     /gene="cgtA"
                     /locus_tag="ACHY3M_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFS4315547.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(11024..11989)
                     /gene="yhbE"
                     /locus_tag="ACHY3M_000573"
     CDS             complement(11024..11989)
                     /gene="yhbE"
                     /locus_tag="ACHY3M_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4315548.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(12116..12373)
                     /gene="rpmA"
                     /locus_tag="ACHY3M_000574"
     CDS             complement(12116..12373)
                     /gene="rpmA"
                     /locus_tag="ACHY3M_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4315549.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12394..12705)
                     /gene="rplU"
                     /locus_tag="ACHY3M_000575"
     CDS             complement(12394..12705)
                     /gene="rplU"
                     /locus_tag="ACHY3M_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4315550.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12964..13935
                     /gene="ispB"
                     /locus_tag="ACHY3M_000576"
     CDS             12964..13935
                     /gene="ispB"
                     /locus_tag="ACHY3M_000576"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFS4315551.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            14162..14440
                     /gene="sfsB"
                     /locus_tag="ACHY3M_000577"
     CDS             14162..14440
                     /gene="sfsB"
                     /locus_tag="ACHY3M_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFS4315552.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14488..15747)
                     /gene="murA"
                     /locus_tag="ACHY3M_000578"
     CDS             complement(14488..15747)
                     /gene="murA"
                     /locus_tag="ACHY3M_000578"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4315553.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15802..16056)
                     /gene="ibaG"
                     /locus_tag="ACHY3M_000579"
     CDS             complement(15802..16056)
                     /gene="ibaG"
                     /locus_tag="ACHY3M_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFS4315554.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(16216..16509)
                     /gene="mlaB"
                     /locus_tag="ACHY3M_000580"
     CDS             complement(16216..16509)
                     /gene="mlaB"
                     /locus_tag="ACHY3M_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFS4315555.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16509..17144)
                     /gene="mlaC"
                     /locus_tag="ACHY3M_000581"
     CDS             complement(16509..17144)
                     /gene="mlaC"
                     /locus_tag="ACHY3M_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFS4315556.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(17163..17714)
                     /gene="mlaD"
                     /locus_tag="ACHY3M_000582"
     CDS             complement(17163..17714)
                     /gene="mlaD"
                     /locus_tag="ACHY3M_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFS4315557.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17719..18501)
                     /gene="mlaE"
                     /locus_tag="ACHY3M_000583"
     CDS             complement(17719..18501)
                     /gene="mlaE"
                     /locus_tag="ACHY3M_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFS4315558.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18509..19318)
                     /gene="mlaF"
                     /locus_tag="ACHY3M_000584"
     CDS             complement(18509..19318)
                     /gene="mlaF"
                     /locus_tag="ACHY3M_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFS4315559.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19528..20505
                     /gene="yrbG"
                     /locus_tag="ACHY3M_000585"
     CDS             19528..20505
                     /gene="yrbG"
                     /locus_tag="ACHY3M_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFS4315560.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPSRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20519..21505
                     /gene="kdsD"
                     /locus_tag="ACHY3M_000586"
     CDS             20519..21505
                     /gene="kdsD"
                     /locus_tag="ACHY3M_000586"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFS4315561.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21526..22092
                     /gene="kdsC"
                     /locus_tag="ACHY3M_000587"
     CDS             21526..22092
                     /gene="kdsC"
                     /locus_tag="ACHY3M_000587"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFS4315562.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            22089..22664
                     /gene="lptC"
                     /locus_tag="ACHY3M_000588"
     CDS             22089..22664
                     /gene="lptC"
                     /locus_tag="ACHY3M_000588"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFS4315563.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22633..23190
                     /gene="lptA"
                     /locus_tag="ACHY3M_000589"
     CDS             22633..23190
                     /gene="lptA"
                     /locus_tag="ACHY3M_000589"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFS4315564.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            23197..23922
                     /gene="lptB"
                     /locus_tag="ACHY3M_000590"
     CDS             23197..23922
                     /gene="lptB"
                     /locus_tag="ACHY3M_000590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFS4315565.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23970..25403
                     /gene="rpoN"
                     /locus_tag="ACHY3M_000591"
     CDS             23970..25403
                     /gene="rpoN"
                     /locus_tag="ACHY3M_000591"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4315566.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25426..25713
                     /gene="hpf"
                     /locus_tag="ACHY3M_000592"
     CDS             25426..25713
                     /gene="hpf"
                     /locus_tag="ACHY3M_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFS4315567.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25831..26322
                     /gene="ptsN"
                     /locus_tag="ACHY3M_000593"
     CDS             25831..26322
                     /gene="ptsN"
                     /locus_tag="ACHY3M_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFS4315568.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26368..27222
                     /gene="rapZ"
                     /locus_tag="ACHY3M_000594"
     CDS             26368..27222
                     /gene="rapZ"
                     /locus_tag="ACHY3M_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4315569.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            27219..27491
                     /gene="npr"
                     /locus_tag="ACHY3M_000595"
     CDS             27219..27491
                     /gene="npr"
                     /locus_tag="ACHY3M_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFS4315570.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27705..28337
                     /gene="yrbL"
                     /locus_tag="ACHY3M_000596"
     CDS             27705..28337
                     /gene="yrbL"
                     /locus_tag="ACHY3M_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFS4315571.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(28334..29062)
                     /gene="mtgA"
                     /locus_tag="ACHY3M_000597"
     CDS             complement(28334..29062)
                     /gene="mtgA"
                     /locus_tag="ACHY3M_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFS4315572.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(29059..29712)
                     /gene="elbB"
                     /locus_tag="ACHY3M_000598"
     CDS             complement(29059..29712)
                     /gene="elbB"
                     /locus_tag="ACHY3M_000598"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFS4315573.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29942..32278)
                     /gene="arcB"
                     /locus_tag="ACHY3M_000599"
     CDS             complement(29942..32278)
                     /gene="arcB"
                     /locus_tag="ACHY3M_000599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFS4315574.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32374..33303)
                     /gene="yhcC"
                     /locus_tag="ACHY3M_000600"
     CDS             complement(32374..33303)
                     /gene="yhcC"
                     /locus_tag="ACHY3M_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4315575.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            33978..38438
                     /gene="gltB"
                     /locus_tag="ACHY3M_000601"
     CDS             33978..38438
                     /gene="gltB"
                     /locus_tag="ACHY3M_000601"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFS4315576.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQRGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38451..39869
                     /gene="gltD"
                     /locus_tag="ACHY3M_000602"
     CDS             38451..39869
                     /gene="gltD"
                     /locus_tag="ACHY3M_000602"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFS4315577.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            40053..41100
                     /gene="yhcG"
                     /locus_tag="ACHY3M_000603"
                     /pseudo
     CDS             40053..41100
                     /gene="yhcG"
                     /locus_tag="ACHY3M_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(41160..41624)
                     /gene="nanQ"
                     /locus_tag="ACHY3M_000604"
     CDS             complement(41160..41624)
                     /gene="nanQ"
                     /locus_tag="ACHY3M_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFS4315578.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(41621..42496)
                     /gene="nanK"
                     /locus_tag="ACHY3M_000605"
     CDS             complement(41621..42496)
                     /gene="nanK"
                     /locus_tag="ACHY3M_000605"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFS4315579.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPATFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(42493..43182)
                     /gene="nanE"
                     /locus_tag="ACHY3M_000606"
     CDS             complement(42493..43182)
                     /gene="nanE"
                     /locus_tag="ACHY3M_000606"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4315580.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(43230..44720)
                     /gene="nanT"
                     /locus_tag="ACHY3M_000607"
     CDS             complement(43230..44720)
                     /gene="nanT"
                     /locus_tag="ACHY3M_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFS4315581.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(44830..45723)
                     /gene="nanA"
                     /locus_tag="ACHY3M_000608"
     CDS             complement(44830..45723)
                     /gene="nanA"
                     /locus_tag="ACHY3M_000608"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS4315582.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLTGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(45845..46636)
                     /gene="nanR"
                     /locus_tag="ACHY3M_000609"
     CDS             complement(45845..46636)
                     /gene="nanR"
                     /locus_tag="ACHY3M_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFS4315583.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            47016..48383
                     /gene="dcuC"
                     /locus_tag="ACHY3M_000610"
     CDS             47016..48383
                     /gene="dcuC"
                     /locus_tag="ACHY3M_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS4315584.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMSTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(48426..48923)
                     /gene="sspB"
                     /locus_tag="ACHY3M_000611"
     CDS             complement(48426..48923)
                     /gene="sspB"
                     /locus_tag="ACHY3M_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFS4315585.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(48929..49567)
                     /gene="sspA"
                     /locus_tag="ACHY3M_000612"
     CDS             complement(48929..49567)
                     /gene="sspA"
                     /locus_tag="ACHY3M_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFS4315586.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(49962..50354)
                     /gene="rpsI"
                     /locus_tag="ACHY3M_000613"
     CDS             complement(49962..50354)
                     /gene="rpsI"
                     /locus_tag="ACHY3M_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4315587.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(50370..50798)
                     /gene="rplM"
                     /locus_tag="ACHY3M_000614"
     CDS             complement(50370..50798)
                     /gene="rplM"
                     /locus_tag="ACHY3M_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4315588.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(51017..52144)
                     /gene="zapE"
                     /locus_tag="ACHY3M_000615"
     CDS             complement(51017..52144)
                     /gene="zapE"
                     /locus_tag="ACHY3M_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFS4315589.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            52338..52736
                     /gene="zapG"
                     /locus_tag="ACHY3M_000616"
     CDS             52338..52736
                     /gene="zapG"
                     /locus_tag="ACHY3M_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFS4315590.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            52890..54257
                     /gene="degQ"
                     /locus_tag="ACHY3M_000617"
     CDS             52890..54257
                     /gene="degQ"
                     /locus_tag="ACHY3M_000617"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFS4315591.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            54347..55414
                     /gene="degS"
                     /locus_tag="ACHY3M_000618"
     CDS             54347..55414
                     /gene="degS"
                     /locus_tag="ACHY3M_000618"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFS4315592.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(55477..56415)
                     /gene="mdh"
                     /locus_tag="ACHY3M_000619"
     CDS             complement(55477..56415)
                     /gene="mdh"
                     /locus_tag="ACHY3M_000619"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS4315593.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            56850..57320
                     /gene="argR"
                     /locus_tag="ACHY3M_000620"
     CDS             56850..57320
                     /gene="argR"
                     /locus_tag="ACHY3M_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFS4315594.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            57685..57948
                     /gene="yhcN"
                     /locus_tag="ACHY3M_000621"
     CDS             57685..57948
                     /gene="yhcN"
                     /locus_tag="ACHY3M_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFS4315595.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(58004..58276)
                     /gene="yhcO"
                     /locus_tag="ACHY3M_000622"
     CDS             complement(58004..58276)
                     /gene="yhcO"
                     /locus_tag="ACHY3M_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFS4315596.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(58368..60335)
                     /gene="aaeB"
                     /locus_tag="ACHY3M_000623"
     CDS             complement(58368..60335)
                     /gene="aaeB"
                     /locus_tag="ACHY3M_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFS4315597.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVEIAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(60341..61273)
                     /gene="aaeA"
                     /locus_tag="ACHY3M_000624"
     CDS             complement(60341..61273)
                     /gene="aaeA"
                     /locus_tag="ACHY3M_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFS4315598.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(61281..61484)
                     /gene="aaeX"
                     /locus_tag="ACHY3M_000625"
     CDS             complement(61281..61484)
                     /gene="aaeX"
                     /locus_tag="ACHY3M_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFS4315599.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            61667..62596
                     /gene="aaeR"
                     /locus_tag="ACHY3M_000626"
     CDS             61667..62596
                     /gene="aaeR"
                     /locus_tag="ACHY3M_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFS4315600.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(62724..64169)
                     /gene="tldD"
                     /locus_tag="ACHY3M_000627"
     CDS             complement(62724..64169)
                     /gene="tldD"
                     /locus_tag="ACHY3M_000627"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFS4315601.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(64325..68125)
                     /gene="yhdP"
                     /locus_tag="ACHY3M_000628"
     CDS             complement(64325..68125)
                     /gene="yhdP"
                     /locus_tag="ACHY3M_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFS4315602.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNLIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(68193..69662)
                     /gene="rng"
                     /locus_tag="ACHY3M_000629"
     CDS             complement(68193..69662)
                     /gene="rng"
                     /locus_tag="ACHY3M_000629"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFS4315603.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(69652..70245)
                     /gene="yhdE"
                     /locus_tag="ACHY3M_000630"
     CDS             complement(69652..70245)
                     /gene="yhdE"
                     /locus_tag="ACHY3M_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFS4315604.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(70254..70742)
                     /gene="mreD"
                     /locus_tag="ACHY3M_000631"
     CDS             complement(70254..70742)
                     /gene="mreD"
                     /locus_tag="ACHY3M_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4315605.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(70742..71845)
                     /gene="mreC"
                     /locus_tag="ACHY3M_000632"
     CDS             complement(70742..71845)
                     /gene="mreC"
                     /locus_tag="ACHY3M_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4315606.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(71911..72954)
                     /gene="mreB"
                     /locus_tag="ACHY3M_000633"
     CDS             complement(71911..72954)
                     /gene="mreB"
                     /locus_tag="ACHY3M_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFS4315607.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(73259..75199)
                     /gene="csrD"
                     /locus_tag="ACHY3M_000634"
     CDS             complement(73259..75199)
                     /gene="csrD"
                     /locus_tag="ACHY3M_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFS4315608.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            75351..76325
                     /gene="yhdH"
                     /locus_tag="ACHY3M_000635"
     CDS             75351..76325
                     /gene="yhdH"
                     /locus_tag="ACHY3M_000635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFS4315609.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            76362..77192
                     /locus_tag="ACHY3M_000636"
     CDS             76362..77192
                     /locus_tag="ACHY3M_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315610.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            78058..78528
                     /gene="accB"
                     /locus_tag="ACHY3M_000637"
     CDS             78058..78528
                     /gene="accB"
                     /locus_tag="ACHY3M_000637"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4315611.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            78539..79888
                     /gene="accC"
                     /locus_tag="ACHY3M_000638"
     CDS             78539..79888
                     /gene="accC"
                     /locus_tag="ACHY3M_000638"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4315612.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(79980..81026)
                     /locus_tag="ACHY3M_000639"
     CDS             complement(79980..81026)
                     /locus_tag="ACHY3M_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4315613.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(81023..81985)
                     /locus_tag="ACHY3M_000640"
     CDS             complement(81023..81985)
                     /locus_tag="ACHY3M_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS4315614.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(82007..82996)
                     /locus_tag="ACHY3M_000641"
     CDS             complement(82007..82996)
                     /locus_tag="ACHY3M_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315615.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(82997..84496)
                     /locus_tag="ACHY3M_000642"
     CDS             complement(82997..84496)
                     /locus_tag="ACHY3M_000642"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS4315616.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(84557..85447)
                     /locus_tag="ACHY3M_000643"
     CDS             complement(84557..85447)
                     /locus_tag="ACHY3M_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4315617.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(85483..86337)
                     /locus_tag="ACHY3M_000644"
     CDS             complement(85483..86337)
                     /locus_tag="ACHY3M_000644"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFS4315618.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT        21564 a        23123 c        21138 g        20673 t
ORIGIN      
        1 cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc
       61 tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata
      121 ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa
      181 gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg
      241 atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac
      301 atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat acctgtatcc
      361 ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct
      421 ttcgctgcta aagcaaaata gttgagattg aaaaacggcg tataggtagc aaatcgcgac
      481 gctagtatat atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa
      541 gcggaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc
      601 ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaag
      661 gcacatagat aagggatata catccccagc attttgacaa cttcatccgg atcactctgg
      721 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg
      781 ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc
      841 ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag caatcgctga
      901 accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga
      961 tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt catccagaaa
     1021 tccaagttca caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt
     1081 ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc
     1141 ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga
     1201 acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat
     1261 ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc agcagaaggg
     1321 aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag
     1381 caactggacg agatacagat acctgacaac cattcctcag accaggacca aaacgaaaaa
     1441 agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta
     1501 agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac
     1561 ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggttgagt
     1621 ttgttcggtt ttcaccggtg cactcagatt ttcccattcg ctacctttat tggttttgtt
     1681 gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca gcagcgccgt
     1741 catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc
     1801 tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag
     1861 gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca acctgttcct
     1921 tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca
     1981 ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca
     2041 ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa aaaggcgaca
     2101 atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg
     2161 cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac
     2221 ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc
     2281 tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc tgcggtgtaa
     2341 cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc
     2401 atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta
     2461 gtagttttat ccagtaggat cacatgaccg gaattctctg caccgatacg ccagcctttc
     2521 tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct
     2581 aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca
     2641 cgtagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg
     2701 actttattgc cttcatggtc aaccataatc acgcgatcgc catcgccgtc gaaggcaata
     2761 ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc
     2821 acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc
     2881 agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca
     2941 atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag
     3001 cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg
     3061 atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag tttggtgccg
     3121 tcgatagaga agaatttaat gccattatcg tagaacgggt tatgcgatgc agatatcaca
     3181 attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg
     3241 ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc
     3301 atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc
     3361 agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc
     3421 cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt
     3481 ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc
     3541 atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc
     3601 gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat
     3661 acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata
     3721 gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc
     3781 gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa
     3841 gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag
     3901 acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg
     3961 gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga
     4021 tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac
     4081 acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc
     4141 aacgtcaatg atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat
     4201 cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat
     4261 tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc
     4321 cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata
     4381 cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt gttacccggg
     4441 ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa
     4501 ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc
     4561 aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca
     4621 tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc
     4681 agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc
     4741 gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat
     4801 ttctcagaga agccccactg ggtcaccatg ttacgtgcca gattggtcgc aactttaata
     4861 tcgttggacg caccggtaga aacatgttcc ggcccataga tgatctcttc tgccagacga
     4921 ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg
     4981 ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcactttg
     5041 tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa
     5101 gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg
     5161 attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca
     5221 gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa accaggagta
     5281 ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg gtacgcgacg catgtgaact
     5341 ttcaggatct gctcacgacc gcgaacatct ggcaagccga ccacaacctg acggtcgaaa
     5401 cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg
     5461 atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt
     5521 tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt
     5581 tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca
     5641 cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac
     5701 ggaactttcg cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga
     5761 ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg
     5821 cgcagatact ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca
     5881 gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt accaaacgac
     5941 atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc
     6001 agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc
     6061 ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga
     6121 accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg
     6181 ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc
     6241 accttacggc cattagactc gctgggccca aagctctgga atactgacat cagcacaacg
     6301 gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat
     6361 tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt
     6421 ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt
     6481 gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt
     6541 agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct
     6601 aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc
     6661 accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg gactttgctg
     6721 tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga
     6781 aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg
     6841 ccgccaattt ggttgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca
     6901 accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag
     6961 gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc
     7021 tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg
     7081 gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag
     7141 atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag
     7201 tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct
     7261 tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca
     7321 tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct tgatcgtgga
     7381 agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct
     7441 ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac
     7501 acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc
     7561 tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata catcaacatc
     7621 ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc
     7681 gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat
     7741 aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg
     7801 ataagtctgt tctttgtcag aatccagatt cagcaccgtt acggtagcac cgaaaataac
     7861 gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca gcttggcttc
     7921 gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt ggtattcggc
     7981 gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg
     8041 gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat
     8101 cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca
     8161 ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc
     8221 gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat tttaccctga
     8281 agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat
     8341 tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg
     8401 tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgctgg
     8461 tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta ttgattacca
     8521 cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc tggcggcgtt
     8581 gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga
     8641 aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg
     8701 tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg
     8761 caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg
     8821 gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg accgcaactg
     8881 tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta tacgcgtggc
     8941 atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag gttctgccga
     9001 agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta cgctgacggg
     9061 atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag
     9121 ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct ggagcggaac
     9181 actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc
     9241 cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac
     9301 ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc gggccgggtc
     9361 ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa acaccattat
     9421 tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt
     9481 gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc
     9541 gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggaaa
     9601 agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac
     9661 agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag tagaaccggc
     9721 ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt ataaagatat
     9781 ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt gctgattaac
     9841 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca tcccagtcct
     9901 catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt
     9961 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    10021 tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag atcagataat
    10081 atttatcttc ccagcccagc gcttcagcaa tcgctttcgc tttctcttca gcttctgcct
    10141 tatccagcag atcgatctta ttgaacacta accaacgcgg tttcgccgcc agatcctggc
    10201 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    10261 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    10321 ggatacccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa
    10381 cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg
    10441 gataatccgc cactttcggt ttagccgccg ataccgcgcg aataaaggtc gatttacccg
    10501 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    10561 gcttatcacc cggcgtaccg ttggttttct gccgtggtgt acggttaacg gacgatttga
    10621 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    10681 tagtcatatc gcccatggtt tcaccagtac cttggtcgat tatacgcgta cctaccggca
    10741 ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac
    10801 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttttcgt
    10861 cggcttccat ccatacgtca ccaccatcac caccgtcgcc gccatccggg ccgcctttcg
    10921 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    10981 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    11041 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    11101 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    11161 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    11221 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    11281 ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc
    11341 agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca attgtacata
    11401 aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg
    11461 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    11521 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    11581 tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata
    11641 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    11701 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    11761 ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct
    11821 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    11881 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    11941 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    12001 tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    12061 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    12121 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    12181 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    12241 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    12301 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    12361 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    12421 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    12481 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    12541 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    12601 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    12661 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    12721 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    12781 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    12841 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcactcta tattcctaac
    12901 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    12961 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    13021 gcaatccttg agcagcttaa ttccgacgtc cagctgatca atcagttagg ctattacatc
    13081 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    13141 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    13201 actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    13261 aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    13321 ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc agaagccgta
    13381 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    13441 gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg
    13501 cagtgttccg ggattctggc tggctgtacg ccggaagagg agaaaggcct gcaggattat
    13561 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    13621 ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    13681 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc
    13741 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    13801 tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat cgcagcgtta
    13861 caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt
    13921 caacgcgatc gttaatcccc tcccctcatc ccgcgcagcg cgcgggatgg ttccagtaaa
    13981 ttccataaaa acacttactc agctctataa cacctgaaaa acaggctgtg catctttata
    14041 tattctgaat attcacactc cttacaggaa ctttttagag caatcggcca tcaggagtat
    14101 agtgatgctc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa
    14161 gatggaaagt aatttcattg actggcatcc cgctgacatc attgcgggtt tgcgcaagaa
    14221 gggaacgtca atggcggcgg agtctcgcag aaatggtttg agttcctcaa cgctggcgaa
    14281 tgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga
    14341 cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag
    14401 aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg
    14461 cgaacggggc tgccagctct cagacgatta ctcgcctttc acacgctcaa tatttgcacc
    14521 taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc
    14581 aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag
    14641 atcggttgcc ataacctgtg cgccagaaag tttttcaaca ccgtgacaaa taacggtatt
    14701 gctttcgatt tcggcatgcg cgcccatacg gctaagctcc ggcacatgca taaagcggtt
    14761 ttcaaagacc gtttcggtga tgaatccggt cccttctgcc accaggttca acagcgtgaa
    14821 ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt
    14881 cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc
    14941 agcgtcacgc agtttcgcca gcacggcgtc gagagtatct ggctgcgcgt tacggcagat
    15001 aattttgccg cgagaaatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg
    15061 tagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt
    15121 gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc
    15181 cggttcacgc gctgcgtttt caataatcgt ggtaccttcc gccagggttg cagcacacat
    15241 gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg
    15301 accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc
    15361 gaggccagaa atgtgtagat caaccggacg cgcaccgatc gtacaaccgc caggcagtga
    15421 aacttgcccc tgaccaaagc gcgctaccag cggccccagt gcccagatag aagcacgcat
    15481 ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac
    15541 agaaccatta cgttctactt tcgcacccag ctggcttagc agcttcattg atgtatcgac
    15601 gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta gagcggcaaa
    15661 aaggataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt
    15721 tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc
    15781 taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc
    15841 aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag
    15901 cgggccatag accgtctgct gttttttaac ccgactcatg ccgtcaaaca actcacccac
    15961 ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg acttcctgga gggagagagc
    16021 gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata
    16081 aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg
    16141 ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat
    16201 ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgcgag
    16261 ggtatacact ttgtcgttta ccccctgaag cgtcacgttg ttgccctgct ttttcgccag
    16321 atcgataaga tggagcagca gtgccagtcc acccgtatcc acgcgggaca cacggctaag
    16381 atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac
    16441 gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga
    16501 ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc
    16561 gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg
    16621 gtgatcatac tgacgccttc agcaatcatg tcgtaagcct gccagtttcc cgtctgggag
    16681 tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta
    16741 acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt
    16801 tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg
    16861 gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc
    16921 gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga
    16981 taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc
    17041 gccgcctcgt ccatcagctt atacggattg gtctggtctg ccgcggttgc cgcactcaga
    17101 ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga
    17161 aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc
    17221 gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga
    17281 tcttccagca ccatcgcaga cttagtgtcc tgaattgtat cgccatcctt caggatagca
    17341 gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc ccccagcagg
    17401 ccggaagtac gaatgctcag cgaactggta tctggaatgt ggttataacg ttgttcaatt
    17461 tccagcgtta cgcgtggcag ataggttttc gggtccagcg taatatccgc cacccggccc
    17521 acaacaacgc caccaatact gaccggagag cgcgctttca ggccgccaat gttatcgaac
    17581 gtcgcataaa gcgtgtaggt cggttcagta cgtatggacg tcacgttcgc cgccttcagg
    17641 caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt
    17701 tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc
    17761 cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga
    17821 cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac
    17881 cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc
    17941 cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagag atccgcccca
    18001 gatccccacg gcgacgaaaa taaccgtcaa cagtggtaat gaaataaccc cagcccagaa
    18061 acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg
    18121 ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc gaaccagcac gcccggcaaa
    18181 caacaacgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc
    18241 cagactggtt tccgcactat aagtggtcag aaccagataa ccttgcagcc ccaacaccat
    18301 tccgatgaac acgccagaaa ccacaataat cagcatcgac aggacgccga cattatagag
    18361 ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa
    18421 taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga
    18481 cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat
    18541 cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta
    18601 cgcgcggatc aggattcgcc tgcaacgcct gggcactgcc atgagcgaca atttttttgt
    18661 ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca
    18721 ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca
    18781 tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg
    18841 caatcgcacg cgccagcgct gcacgccgcg ccatcccacc agaaagttca gaaggcatta
    18901 gcttagccgc tccacgcagc cccacggcct ccagcttcat catcactgta ctatgcaaca
    18961 atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca
    19021 tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttgcgc actgtataca
    19081 ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg
    19141 gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca
    19201 tgattgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat
    19261 tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa
    19321 ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa
    19381 catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta
    19441 gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg
    19501 cgagtatacc tgaagaaagg atgttagatg cttttagcta cggcactgtt aattgttggt
    19561 ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga
    19621 acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta
    19681 ccagaagtca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt
    19741 acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt
    19801 cgtcctttta ccgtccattc tgatgttcta cgccgtgaat tacccttaat gttgttggtc
    19861 agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgcagcga tggtatcttt
    19921 ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggctgaa
    19981 cgtcagggga ctgacagcct gaccagagag cagcttgcag agctgccgcg tgacggcgga
    20041 ttgcccgtcg cgtttttatg gctcggcatt gcgcttatca tcatgccagt ggccacgcgg
    20101 atggtggttg ataacgccac ggtgctggcg aattactttg ccatcagcga gttgacgatg
    20161 ggtctgacgg caattgctat cggaaccagc ctgccggaac tggcaaccgc aatagcgggg
    20221 gttcgcaaag gtgaaaacga cattgctgtc ggaaatatca ttggcgcaaa catttttaat
    20281 attgtcatcg tgttgggttt acccgcgctg ataacgccag gagagattga tccactggcg
    20341 tacagtcgtg actacagcgt gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg
    20401 cggcgctccc cacaaccgag ccgtggtgta ggggtattat taactggcgg atttatcgta
    20461 tggctggcga tgttgtactg gttatcgcca atactcgttg aataactgga aacgcattat
    20521 gtcgcacgta gagttacaac cgggttttga ctttcagcaa gcaggtaaag aagtcctggc
    20581 gattgaacgt gaatgcctgg cggagcttga tcaatacatc aatcagaatt tcacgcttgc
    20641 ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg
    20701 gcacatcggg cgcaaaatgg ccgcaacgtt tgccagcacc ggtacacctt catttttcgt
    20761 ccatcctggt gaagccgcgc atggtgattt aggcatggtc accccacagg atgtggtgat
    20821 tgctatctct aactcaggtg aatccagcga aatcacggcc ttaattccag tgcttaagcg
    20881 tcttcacgta ccgttaatct gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc
    20941 agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt ccgttagggc tggcaccgac
    21001 cagcagcacc accgccacgc tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc
    21061 acgcggcttt actgctgaag attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa
    21121 acttctgctg cgcgtaaacg atattatgca tacgggcgat gagatcccgc atgttaagaa
    21181 aacggccagt ctgcgtgacg cattgctgga agttacccgc aaaaatcttg gtatgactgt
    21241 catttgcgat gacaatatga tgattgaagg catctttacc gacggtgatt tacgccgtgt
    21301 cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg
    21361 aatacgtgtg cgccctggca ttctggccgt tgaggcactg aacttaatgc agtcccgcca
    21421 tatcacctcc gtgatggttg ccgatggcga ccatttactc ggtgtgttac atatgcatga
    21481 tttactgcgt gcaggcgtag tgtaaagatt caaggataaa caacaatgag caaagcaggt
    21541 gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg ttatggcaaa agcagagaac
    21601 attcgtctgc tgatcctcga cgttgatggc gtactgtcag atggcctgat ttatatgggc
    21661 aataacggcg aagagctgaa agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg
    21721 ctcacctctg atattgaagt cgctatcatt accgggcgaa aggctaaact ggtagaagat
    21781 cgttgtgcca cattggggat cactcacttg tatcaggggc agtcaaacaa actgatcgcc
    21841 tttagcgatt tgctggaaaa actggcgatt gccccggaaa atgtggctta tgtcggcgat
    21901 gatctcatcg actggccggt aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgcg
    21961 catccactgt tgatcccgcg cgccgattac gtgacgcgca ttgctggcgg tcgtggcgca
    22021 gtgcgcgaag tttgcgactt attactcctg gcgcagggca aactggatga agccaaaggg
    22081 caatcgatat gagtaaagcc agacgttggg ttatcattgt gctatcactg gcggttctgg
    22141 tgatgatcgg cattaatatg gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca
    22201 atgatcccac ctataaaagc gagcatacgg acacgctcgt ctataaccca gaaggggcac
    22261 taagctatcg attgattgct caacacgttg aatattattc cgatcaggcc gtttcgtggt
    22321 ttacgcagcc ggtacttacc acgtttgata aggataaaat cccgacatgg tccgtaaaag
    22381 cagataaagc caagctgacc aatgaccgga tgctctattt atatggacac gttgaagtca
    22441 acgcactcgt gccggactct caacttcgca gaatcacgac ggataacgcg cagatcaatc
    22501 tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt atacggaaca acatttaact
    22561 ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa cgccgagctg attgaaaagg
    22621 ttagaacatc ctatgaaatt caaaacaaac aaactcagcc ttaatcttgt gcttgccagc
    22681 tcacttctgg ccgccagcat tccggcattt gccgtaaccg gagacactga tcagccgatc
    22741 cacattgaat cggaccagca atctcttgat atgcaaggca acgtggttac ctttaccggt
    22801 aatgtcatcg tcacccaggg caccatcaaa attaatgccg acaaagtggt tgttacccgt
    22861 ccgggcggcg aacaaggtaa agaagtgatt gacggctacg gtaaaccggc aacgttctac
    22921 cagatgcagg acaacggtaa accggttgaa ggtcacgctt cccagatgca ctacgaactg
    22981 gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc agcaggttga tagcaacatt
    23041 aagggcgata agatcactta cctggtaaaa gagcagaaaa tgcaggcttt cagcgacaaa
    23101 ggcaagcgcg taaccaccgt tctggtgccg tcgcagctgc aggacaaaaa caacaaaggc
    23161 cagaccccgg cacagaagaa gggtaattaa ttcgttatgg caacattaac tgcaaagaac
    23221 cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc
    23281 ggggaaattg tcggtctgct ggggccaaac ggtgccggta agaccaccac tttctatatg
    23341 gttgtaggca ttgtgccgcg cgatgcgggc aacatcatta ttgatgatga cgatatcagt
    23401 ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc
    23461 attttccgtc gcctcagcgt ttacgataac ctgatggcgg tactgcaaat tcgtgacgac
    23521 ttgtctgctg aacaacgtga agaccgcgcg aacgagctga tggaagagtt tcacattgag
    23581 cacctgcgtg acagcatggg gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt
    23641 gcccgcgcac tggctgcgaa tccgaaattt attctgctcg acgaaccgtt tgccggtgtt
    23701 gacccgatct cggttatcga cattaaacgc atcattgagc acctgcgcga cagcggcctg
    23761 ggcgtgctga tcactgacca caacgtgcgt gaaacactgg cggtttgtga acgcgcttat
    23821 atcgtcagtc aggggcattt gatcgcccac ggcacgccta cagaaatctt acaagacgaa
    23881 cacgttaagc gtgtatacct tggggaagac ttcagactct gatagggtag aagtttgcga
    23941 cgttttagca ggagagtacg attctgaaca tgaagcaagg tttgcaactc aggcttagcc
    24001 aacaactggc gatgacgcca cagctccaac aggcaattcg tctgttgcag ttgtcgacgc
    24061 tggaacttca gcaggagcta cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg
    24121 acactcatga agaaatcgac acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg
    24181 acgcgctcga acaaaaagag atgccggaag agctgccgct cgatgccagt tgggacacca
    24241 tttacaccgc tggtacacca tccggcacca gcggtgacta cattgacgat gagctgccgg
    24301 tctatcaggg cgaaacgacg cagaccttgc aggattacct gatgtggcag gtcgagctga
    24361 caccgttttc cgacactgac cgcgctattg ctacctctat cgtcgatgcc gttgatgaca
    24421 ccggttatct gactgtcccg ctggaagata ttctcgaaag tatgggcgat gaagagatcg
    24481 acatcgacga ggttgaagcc gtccttaagc ggatccaacg gtttgatccg gtcggtgtag
    24541 cggcaaaaga tctgcgtgac tgcctgctaa tccaactctc ccaattcgat aaaaccacgc
    24601 cgtggctgga agaggccaga ttgatcatta gcgatcatct cgatctgtta gccaatcacg
    24661 acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca
    24721 atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat ccagactggc gaacctgagt
    24781 atgtcattcc agatgtgctg gtgcgtaagc ataacggtca ctggacagta gaactcaaca
    24841 gtgacagcat tccgcgtctg caaatcaacc agcactacgc ctcgatgtgc aataacgctc
    24901 gcaatgatgg tgacagccag tttatccgca gcaatctgca ggatgccaaa tggttgatca
    24961 agagtctgga aagccgtaac gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc
    25021 agcaagcctt ctttgagcaa ggcgaagaat atatgaaacc gatggtactg gccgatatcg
    25081 cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc
    25141 atagtccacg aggcattttt gaactgaagt atttcttttc cagtcacgtc aataccgagg
    25201 gcggcggcga agcttcctcc acggcgattc gtgcgctggt gaagaaatta atcgcggcgg
    25261 aaaacccagc gaaaccgttg agcgacagca agttaacctc tttgctgtcg gaacaaggta
    25321 tcatggtggc acgccgcact gttgcgaagt accgagagtc tttatccatt ccgccgtcaa
    25381 accagcgtaa acagctcgtt tgacccaacc gataaggaag acactatgca gctcaacatt
    25441 accggaaata acgtcgagat caccgaggca ctgcgcgaat ttgttacagc caaatttgcc
    25501 aaacttgagc aatattttga ccgaatcaac caggtctatg ttgttctgaa agtggagaaa
    25561 gtcacccaca cctcagatgc aacactgcat gtaaacggtg gtgaaattca tgccagcgcg
    25621 gaaggtcagg acatgtacgc tgccattgat ggcttaattg ataagctggc acgccagttg
    25681 accaaacata aagataaact gaaacaacac taattgtccg ggcaattagc atgtgcatgg
    25741 cggcctgttg tgcggcacaa cgggccattt gtacggttaa tgctccgagc ctgttccact
    25801 gtttgagtgg gcaggttctt aggtgaaatt atgacaaata atgatacaac tctacagctt
    25861 agcagtgttc ttaacaggga atgtacgcga agccgcgtcc actgtcagag caaaaaacgc
    25921 gccctggaaa tcatcagcga gctggcggcg aaacaactta gcctgccgcc tcaggtggtt
    25981 tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc
    26041 attccgcatg gcaaactgga agaagatact ctgcgcgccg ttggcgtttt cgttcagctc
    26101 gaaacgccta tagctttcga tgccatcgac aaccaaccgg tggacttact ttttgccctg
    26161 ctggtgccgg cagaccaaac taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt
    26221 ctggcggaca aaaccatctg ccgccgttta cgcgcagccc agagcgatga agagctgtat
    26281 caaatcatta cggataccga aggtactccg gatgaagcgt agttattcgg taatgtctct
    26341 tttagacgtt gtgaggagaa acagtacatg gtactgatga tcgtcagcgg acgttcaggt
    26401 tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg gtttttactg cgtggataac
    26461 cttcccgtag tgttgttacc cgatctggct cgaactctgg ccgatcgcga gatttctgcc
    26521 gccgtcagca ttgatgttcg taatatgccg gagtcaccag aaatattcga acaggcgatg
    26581 agtaacctgc ctgacgcttt ctcaccgcaa ctactgttcc tggatgccga ccgtaatacc
    26641 ttaattcgtc gttacagtga cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg
    26701 ctggaaagtg ctatcgacaa agaaagcgat ttgctggagc ctctgcgttc gcgagcggat
    26761 ctgattgtcg atacctcaga aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt
    26821 ctgctgggta aacgtgaacg cgaactgacc atggtctttg agtctttcgg cttcaaacac
    26881 ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg
    26941 gatccgaaac tgcgtccaat gacaggtctt gataaacctg tcgccgcgtt cctcgaccgc
    27001 cacacagaag tacacaattt tatctaccag acgcgaagct atcttgaact atggttacct
    27061 atgctggaaa ccaacaaccg tagctacttg acggtcgcca ttggttgtac cggcggaaag
    27121 caccgttcgg tgtatattgc agagcaactg gcagactact tccgctcgcg cggtaaaaac
    27181 gtccagtcac gccatcgtac gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg
    27241 aaatcacaaa caagctgggc atgcatgccc ggcctgcaat gaagctgttt gaattaatgc
    27301 agggttttga cgctgaagtg ctgttacgta atgacgaagg caccgaggcc gaagccaaca
    27361 gcgttattgc gctgctgatg ttggattctg ccaaaggacg gcagattgaa gttgaagcga
    27421 ccggcccaca ggaagaggaa gcactggccg ccgttatcgc cctctttaac tctggttttg
    27481 atgaagatta atcttcatca ctttgacata caactccctt caaactcccc ccgacaataa
    27541 gaaaatcacg tactgaaatc gttctcaatc aacgtcattt gtacattttg tgcgcttttc
    27601 actttcagaa gaaccttaag aaaaccttaa gaggcattgt ttaggttttg tttaagttaa
    27661 tcgaccatac tggagatcgt cagaaaatat ttccaggaga tggcatgatt cgcttatctg
    27721 aacaaagtcc cctgggcacc ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc
    27781 aacgctgtat caagattgtc taccatcgtg gcgatggtgg cgataaagag atccgccgtg
    27841 agttaaagta ctacgcgcat cttggtcgcc ggttaaaaga ctggagtgga ataccgcgct
    27901 atcacggtac cgttgagacg gattgcggaa ccggttatgt ctacgatgtg attgcagatt
    27961 ttgacggcaa accctccatt acgctgaccg aatttgctga acaatgccgt tacgaagaag
    28021 acattgcaca actgcgccag ctactgaaac agctgaagcg ttatttgcag gataaccgta
    28081 tcgtgacgat gtcgctgaag ccgcagaata tcctctgtca ccgcatcagt gagtcagagg
    28141 ttatcccggt cgtctgtgac aacattggtg aaagcacatt gattccactg gcgacctggt
    28201 cgaagtggtg ttgtctgcgt aagcaagagc ggttatggaa acgatttatc gcccaaccgg
    28261 cgctggctat tgccctgcag aaagatctgc aacctcgtga gagcaaaacg ctggccctca
    28321 cttcgcgcga agcttaatcc agctggtgtt gttgcataaa cggctcaccg cctaactgat
    28381 acatctgccg taaaatccac gcctgacggc tacgcacgta gcccgatggc gaggagactt
    28441 tgaaacgaag tggattaggt aatacagctg ccagtaatgc agcttccgac cgggtaagtt
    28501 tgctcgcggg tttgtggaaa taacgttgtg ccgcagcttc gacgccaaac acaccgtcgc
    28561 caaattcggc gatattcagg taaaccgtca ggatacgctt tttgctccag actgtttcta
    28621 tccccagcgt taatccagct tccagccctt ttcgtaccca gctacgccca tcccataaaa
    28681 agagattttt ggccgtctgc tgagaaatcg ttgaagcacc gcgaatacgg ttttcattgc
    28741 gctcgttgtg cgccagggct ttctcaatgg aagcgacatc aaagccccag tgctcaggaa
    28801 atttctgatc ttctgcggca atcaccgcca gtcccatcca cggcgagatt tgatccatac
    28861 tgacccagtc agaatgtgcc acgtaacgaa aattgccatg cagccaggcg ctgacctgtc
    28921 gctcgaccat taccgctgag aagggaacag gcgcaacact aaacaacgcg atgcccccgc
    28981 cccagaaaac ggcgaggaca accattaacc gcaaaagaaa acggcgaacg aaactaaaca
    29041 ccgttaagcg gcttttactc attcagccag aaccagcacg cgggaaacca gcttatcaat
    29101 gccgctcgcc gcttctgcaa tgttctgcgc cagcatatat gctggggtgg tgacaatctt
    29161 attgtcttca tcaaccacga tatcatcgac aggacagggc acatgctccg cgcccatctc
    29221 ttccagcact tctgcggtat cgatatcagt accgatggtc aaacgcagcg ggaaatcgaa
    29281 aattttcggc agcatcgccg gggcaataca cataaaacca agcggttttc cggcctgatg
    29341 catcgcttgt gccagcgcct ttaattcacg gtcaacggtg cattcgctac caagagtggc
    29401 aaaattgctt aaattcttcg ccgcgccaaa cccccccggc acaatcaacg catccagttc
    29461 agcggcatcg gcctgggcca gaggacggat ttcaccgcgc gttattcgtg ccgcttcaat
    29521 cagcacgttg cgcgtttccg tcatcgcttc gccagttaaa tggttgataa catcaacctg
    29581 ctgcttatcc ggtgcaaagc agaccgcctg cgcaccgctg cgtgaaatag ctaacaacgt
    29641 caacaccgct tcatgaattt cagaaccgtc atagacgccg catccgctca gaattacgcc
    29701 aattttcttc attgtgatca tcctttcgca attgactgaa acacattaat ttttttaata
    29761 aaaatggtac gcatcacaca tttaactgat tcatgtaaca aatcatttaa gttttgctat
    29821 cttaactgcg tgcggcctga aaaacagtgc tgtgcccttg taactcatca taataattta
    29881 cggcgcagcc aagatttccc tggtgttggc gcagtattcg cgcaccccgg tctagccggg
    29941 gtcatttttt agtggctttt gccacccacg ctttcagcac ttctacgtcg tgacgccact
    30001 cttctttcat ctcttcaatc cattcaccga cgttatcttc ccaggcagga aggtccggag
    30061 actgaatttg ctgacccagc tgttgcagat ggcgtaaccc cactgacccc gccgcacctt
    30121 taattttatg tccttcctct acaatgcctt ttttatcctg cgccgtcaga ttcgactcca
    30181 gcacgctgac atagcccggc atcatcttct caaacaccgc taacccgtcg gtgatcagct
    30241 tcggtcctac aagttcgaga tactgttcca gcatgggaat atcgagcaat gcttctgatt
    30301 tactgttctc ttctgtcgtc accgtactct cctcatcatc ctgggtatcc cagaatttct
    30361 tgatcatcgc ggttagcgcc ggaaccgaaa gcggcttact cagcacatca tccattccag
    30421 cattgaggta ctcttgtttg tctttcagca cgttagcggt taaggccacc agcggcggta
    30481 aatcctcgcg cggataacgc ttcgtcagtt cacgagagat atccagcccg gtcatatctg
    30541 gcaactgaat atccagcaac accaggtcgt attcgcccgg tttaaacatc tccagcgccg
    30601 ccttgccggt catggcgaca tcaacgctgt tacctaattt ttccagcaca gaacgcgcaa
    30661 caatcacgtt cagttcaatg tcttccacca gcagcacatt cagcgccggt aaaggcatat
    30721 cgtcttcatc aaacgcatca tcgacctctt ctgctaccga cggtgcgtgg atcgtcaacg
    30781 taaaggttga acctttgccc tgttcgctgg taaccgtaat atcgccgccc atatttttcg
    30841 ccagacgacg agaaacggcc agaccaatac cggtgccggt ggcaggttta ccgccatgac
    30901 tgtctttcac ctggtaatac atggcgaaaa ttttatccag ctcatcctgc ggaatgccga
    30961 taccagagtc ttccacttca aaatgcagca tatcgccttc atcgtagcgc acgcgcacgg
    31021 taacctggcc ttgctgggtg aatttgacgg cgttactgat gaggttccac aggatctgcc
    31081 gtaaacgcgt cccgtcggta atgacctgat gcggtaatgg cagcgtcggc tccaggttaa
    31141 agcgcaatcc tttttgttgc gcctgcaatg cggagagatt ttccagatcg gcaaggaagc
    31201 tggtgaaatc aaccggttga ttatcaagct ggaccttgcg ccgttccatc ttatccatgt
    31261 cgataatatc gttaaagata ttccccagcg tgacggccga aacatggatg gtcttgagat
    31321 atttttcctg ctcggcggtg agttcggtat ccagcagaat acggctcagg ccgacgatac
    31381 cgttcagcgg tgtacgcaat tcgtgactga tggtggagat aaacgtcgtt ttgtcgcggc
    31441 tggcccgttc aagcgcatcc tgataccgct tacgctcggt aatgtcgcga ccaaagccca
    31501 tcaaaccgtg acgtttaccc acgcggtcgt agtacggcac tttacggatt tcaaagcagg
    31561 ctttgcgccc gtccgggtaa tccagccact gttcataggt cagtgacaca ttatgacgga
    31621 acactttttc atcggtttca atgacttttg cggcggcttc cggtgagtaa acatcagcag
    31681 gtttcaggtg aaccagttgt ttttcgcttt ttccggtcag cagctccatc gcgcggttac
    31741 agccggaaaa ctctttatct tcgttacgat aaaaaaccag gtcgggtgaa gcatcaagga
    31801 aggaacgtaa gaatgaggat tgctgctcga gctgaatttg tgtctcttcg cgctctttga
    31861 tttcaatttt cagttggccg aaggtttcct gcagttctgc ttccgctttt tcacgaacgg
    31921 cgatttcctg atttagctgg gcaatattat cttttaactg aacgttgagg ctcaaatcgc
    31981 gctcgcgcat ctcctccagt ttttgcacca gccgtgacag acgttgtcgt gactcctcca
    32041 gttgctcgac gaccaccgat agaaagtaga ccgcccacgg cgtaatcagc aaaccaaaga
    32101 agatagaacg aataacatca atgctttcga cctgaccatg cagcaccatg gttaccgcca
    32161 tttgtaccac aatggcaaga acgacgaggg ccagcgccag caacattgag aagcgcacca
    32221 gacctaactt catcatcagg tcaacataat actgcgccag cagacgaatt tgcttcatta
    32281 gggaattcct tcacgacaac ctgccccaat aatacccaat tctgacagtt acgttataaa
    32341 ttgtgtgaga aatgcggaat tcccctcaac gacctactcc gttggaggta tccacggacg
    32401 ttccagcgcc gatccctgta cgccatgttc attcagatag cgatccagct cgaccattcc
    32461 cgtccatcga ttttcgcacc acagaggagc aagcagcgtc gggcgacggg cgctggcgga
    32521 aatgcggtgg tagatcactt ccggtggcgt atggcgaatc atttctccgg cagtgagcgt
    32581 gtaatcctcc agttcaatac cgttcaaacg ccccgcttcc caggctttcg ccataatgct
    32641 gcctttcaca atatgcagcg gatgcagctt tatgccgtcc acgccggttt caaccactcg
    32701 ctccagcgtt tgcaaacatt cagcctgccc ttcgccaggc aggccgacaa ttaagtggga
    32761 acacaccttc agcccgcgct gacgtgccag ctgggttgta cgctgataac aggcaaaatc
    32821 atggccgcgg ttgatgcgat gcagtgtttt gtcgtgggcg gtttgtagcc ccagctccag
    32881 ccacacttcg taaccctggt ccttatattc gcaaagcaga tccagcaccg catccggcac
    32941 gcagtccggg cgggtaccaa cacacaaacc gacaatattg gcctggctca ccgcctgctg
    33001 atacatagaa cgcagcacct gaacttccgc aaaggtgctg gtatacgcct gaaagtaggc
    33061 cagatagcgt ttagcgcggt taactaaatt cgcctggtgc gccagttgct cggcaatgga
    33121 acgatgctgc tgcgcttcat cggcaaacga ggcaacatta cagaatgtgc agccaccacg
    33181 cccgatggta ccgtcacggt tagggcagct aaaaccgccg tgcagcgtca gcttatgcac
    33241 cttttgccca taacgacggg tgagatcacc accaaacata ttgactaatt tctgtaactg
    33301 cataatctga tagaccgcgc cttgaaaaga ggccaaagcc tgccattttt agctcaattc
    33361 ggcgatgacc tggatcaatc gtcccggcct gcttttatca actgcataat caatcaaaat
    33421 taccgaaatt tcatgcataa tcacataaat cacttttgct tatcttgtgt cagatttttt
    33481 tatctcctta tggattttag gcaaaaacag tagcatgaaa cgtcattacc aattaaggca
    33541 gtataaaatg ctggttttct cgtcagttca aggcaggata agggttaaca cgcctttatg
    33601 acagtcagga attgactgtt tctctaacga cttccctttt agccttaaag ataaaatcta
    33661 ttttaatttc agtcatttaa taaagaattt tgcgctaaag cacatttctg taccaataag
    33721 cttgccattt gacctgtatc agctttcccg ataagttgga aatccgctgg aagctttctg
    33781 gatgagcagc ctgctcatca tatttatgca gtaattgaga tcccctcttc accgtattaa
    33841 ccgatgcgaa aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc
    33901 gtcgccacgc tctttctgtg cccgtgcgca gcggttcgga agtggggttc ccgcagagcc
    33961 tgggggaggt tcacgatatg ttgtacgata aatcccttga gagggataac tgtggtttcg
    34021 gcctgatcgc ccacatagaa ggcgaaccta gccacaaggt agtgcgtact gcaatacacg
    34081 cactggcccg catgcagcac cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt
    34141 gcggcttgct gttacaaaaa ccggatcgct tttttcgcat cgttgcgcag gagcgcggct
    34201 ggcgtttagc aaaaaactac gccgtcggga tgctattcct gaataaagat cctgaactcg
    34261 ccgctgccgc acgccgcatc gttgaagaag aactgcaacg cgaaaccttg tcgattgtgg
    34321 gctggcgtga tgtccccact aacgaaggcg tgctgggtga aatcgccctc tcctctctgc
    34381 cacgcattga gcaaattttt gtgaacgccc cggcaggctg gcgtccgcgc gatatggagc
    34441 gccgtctgtt tatcgcccgc cgccgcattg aaaagcgtct cgaagccgac aaagacttct
    34501 acgtctgtag cctgtcgaat ctggtgaaca tctataaagg tctgtgtatg ccggcggatc
    34561 tgccgcgctt ttatctggat cttgcggacc tgcgtctgga atcggccatc tgcctgttcc
    34621 accagcgctt ctccactaac accgtaccgc gctggccgct ggcgcagccg ttccgctacc
    34681 tggcgcataa cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta
    34741 cctataaatt ccagacgccg cttatccctg atctgcacga cgccgcaccg ttcgtcaacg
    34801 aaaccggctc tgactccagt tcgatggata acatgctgga actgctgctg gcaggcggga
    34861 tggatatcat ccgcgccatg cgtctattag taccacccgc ctggcagaac aacccggata
    34921 tggacccgga actgcgtgcc ttctttgact ttaactccat gcatatggag ccgtgggatg
    34981 gcccggcggg catcgtgatg tccgatggtc gcttcgctgc ctgtaacctc gaccgtaacg
    35041 gtctgcgtcc ggcacgctac gtcattacca aagacaagct catcacctgc gcctctgaag
    35101 tcggtatctg ggattatcag cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg
    35161 aactgatggt gatcgacacc cgcagtgggc gtattctgca ctcggcagaa accgatgacg
    35221 atctgaaaag ccgccatcca tataaagagt ggatggagaa aaacgtccgc cgactggtac
    35281 cgtttgaaga tctgcccgat gaagaagtgg gtagccgcga actggacgac gacacgcttg
    35341 ccagctacca gaaacagttt aactacagcg cggaagagct ggactccgta attcgcgtac
    35401 tgggcgaaaa cggtcaggaa gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc
    35461 tctccagtca gccgcgcatt atttacgact acttccgcca gcagtttgcc caggtgacta
    35521 acccgccaat cgacccgctg cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc
    35581 gtgaaatgaa cgtcttttgc gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc
    35641 cgattctgct ctactccgat ttcaaacagc tcacgacgat gaaagaggag cactaccgcg
    35701 cagatacgct ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag
    35761 agctgtgcga caaagccgaa aaaatggtac gtagcggcac cgtgctgctg gtgctctccg
    35821 accggaatat cgctaaagat cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc
    35881 agacccgtct ggtcgatcaa agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca
    35941 gcgcccgcga tccgcaccac ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc
    36001 cataccttgc ctatgaaacg ctgggccgcc tggtagacac ccatgcgatt gccaaagatt
    36061 atcgtaccgt gatgctcaac taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt
    36121 ccaaaatggg catctccacc atcgcctctt accgctgctc gaaactgttt gaagcggtcg
    36181 gtctgcacga tgatgtagtg ggcctgtgct tccagggggc ggtcagccgc attggtgggg
    36241 cgagctttga agacttccag caggatctgc tgaacctgtc gaaacgtgcc tggctggcgc
    36301 gtaagcccat cagccagggc ggcctgctga aatacgtcca cggcggcgaa taccacgctt
    36361 acaacccgga cgtggtgcgc acgctgcaac aggcggtaca aagaggcgag tacagcgact
    36421 atcaggaata cgcgaagctg gttaatgagc gtccggcaac cacgctgcgc gatctgctgg
    36481 caattacgcc gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt
    36541 ttaaacgctt tgataccgcc gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg
    36601 cgctggcgga agcgatgaat agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag
    36661 acccggcgcg ctatggcact aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct
    36721 tcggcgttac tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg
    36781 gcgcgaagcc gggcgaaggc ggtcagttgc cgggcgacaa agtcactcct tacatcgcca
    36841 aactgcgcta ttcggtgccc ggagtgacgc tgatctcccc gccgccgcac cacgatatct
    36901 actctatcga ggacttagcg cagctcattt tcgacctcaa gcaggttaac ccgaaagcga
    36961 tgatctccgt gaagctggtt tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa
    37021 aagcttatgc cgatttaatc accatcgcag gctatgacgg tggcaccggc gcaagcccgc
    37081 tgtcgtcagt gaaatacgca ggttgcccgt gggagctggg gcttgttgaa acccagcagg
    37141 cgctggttgc caatggtctg cgtcataaga tccgtttgca ggtcgatggc ggcctgaaaa
    37201 ccggtgttga tatcatcaaa gcggcgattc tcggcgcaga aagcttcggc ttcggcactg
    37261 gcccgatggt ggcgctcggc tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa
    37321 cgggtgtagc aactcaggat gacaaactgc gtaagaacca ctatcacggc ctgccgttca
    37381 aggttaccaa ttactttgaa tttattgccc gtgaaacccg cgagctgatg gcacagctgg
    37441 gcgtaacgcg tctggtggat ctgattggtc gcaccgacct gctgaaagaa ctggacggtt
    37501 tcaccgccaa acagcaaaaa ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc
    37561 caggtaaggc actctactgc accgaaaaca acccaccgtt tgataacggc ctgctgaacg
    37621 cgcagttgct gcaacaggcg aaaccgtttg tcgatgaacg ccagagcaaa accttctggt
    37681 tcgatattcg caacaccgac cgttctgtcg gcgcgtcgct ttcaggctat atcgcccaga
    37741 cgcacggcga tcaggggctg gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag
    37801 gccagagttt cggtgtgtgg aacgcgggcg gcgtggaact gtacctgacc ggggatgcca
    37861 acgactatgt cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct ccggttggtt
    37921 ccgccttccg cagccatgaa gcaagtatta tcggcaacac ctgcctgtat ggcgcgaccg
    37981 gtggtcgtct gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg
    38041 ctatcaccgt ggtagaaggc attggcgaca acggctgtga atatatgacg ggcggtattg
    38101 tctgtattct gggtaaaacc ggcgttaact tcggtgcggg catgaccggc ggcttcgctt
    38161 acgttctcga tgaaagcggt gatttccgca aacgcgttaa cccggaactg gtcgaggtct
    38221 taagcgttga cgatctggcg atccatgaag agcatctgcg cggtcttatc accgagcatg
    38281 ttcagcatac tggctctcag cgcggtgaag agattctggc gaactggtca accttcgcca
    38341 ctaaatttgc gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg ggtcatcgta
    38401 gtcgtagcgc agcagagttg cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga
    38461 atgtttatca atttatcgac ctgcagcgcg ttgatccgcc aaagaaaccg ctgaagatcc
    38521 gcaaaattga gtttgttgaa atttacgagc cgttttccga aggccaggcc aaagcgcagg
    38581 ctgaccgctg cctgtcgtgc ggcaacccat actgcgagtg gaaatgtccg gtacacaact
    38641 acatcccgaa ctggctgaag ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt
    38701 cgcaccagac caaccccctg ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt
    38761 gcgaaggttc ctgcactctg aacgatgagt ttggcgcggt gaccatcggc aacattgagc
    38821 gttatatcaa tgataaagcg ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc
    38881 aaaccggtaa aaaagtggcg attatcggcg caggcccggc aggtctggcg tgtgcggatg
    38941 tcctgacgcg taacggcgta aaagccgttg tcttcgaccg tcatccagaa attggcgggc
    39001 tgctgacctt cggtattccg gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg
    39061 aaatcttcac cggcatgggt attgaattca aactcaatac cgaagtgggc cgcgacgtgc
    39121 agctggacga tctgctgagt gattacgatg ccgtcttcct tggtgtcggg acttatcagt
    39181 caatgcgcgg cgggctggaa aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc
    39241 ttatcgccaa caccaaacag ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca
    39301 gcatggaagg caaacgcgtg gtggtccttg gcggtggcga cactgcgatg gactgcgtgc
    39361 gtacgtccgt gcgccaggga gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga
    39421 acatgccggg ttcccgccgc gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat
    39481 tcaacgtcca gccactgggg attgaagtga acggtaacgg taaagtcagc ggcgtaaaaa
    39541 tggtacgtac cgaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg
    39601 caggttccga acatatcgtt ccggcagatg cagtgatcat ggcgtttggt ttccgtccac
    39661 acaacatgga atggctggca aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg
    39721 ccccggaagg cagcgacaac gccttccaga ccagcaaccc gaaaatcttt gctggcggcg
    39781 atatcgtccg tggttccgat ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag
    39841 acggtattat gaactggctg gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag
    39901 agtcggcgct gaaattctcc tttctttctg gaaagcgcct cgcataatct gaagaaaacc
    39961 aatgtaatac gctcatctgg attgttaccg gtagatgccc ggcgtatagc tgattccatt
    40021 attcaacgca gcgccatata aagggacaga taatggaatc gctctcagag agaacctcaa
    40081 caggctacca gcaaatccac gacggtatta ttcatctggt cgatagcgcc cggacggaaa
    40141 cggtacgtag cgttaacgcg ctgatgaccg cgacatactg ggaaattggc cgacgaattg
    40201 tcgaatttga acaaggtggc gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac
    40261 tatcaaagga tttaagtcta aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa
    40321 tgaggctttt ttacctcttt tttcaacatg ttgaaattcg ccagacagtg tctggcgaat
    40381 taacacctgc cctggtccac ctacgtccat ttactttccg ttaaaaacgc tgacgcccgc
    40441 agcttttatg aaaaagagac gctccgctgt ggctggtctg ttcgccagct agaacggcaa
    40501 attgcgaccc aattttatga gcgaacacta ctgtcacatg acaaatcagc catgctgcaa
    40561 caacacgctc ctaccgagac gcatattctt ccgcaacagg cgatacgcga tccctttgtg
    40621 ctcgaatttc tggaattgaa agatgaatat tcagaatctg atttcgagga ggcgctgatc
    40681 aaccacctga tggatttcat gctggaactt ggggatgatt ttgcctttgt tggtcggcag
    40741 cgaaggttac gcattgatga caactggttt cgtgtcgatc tgctgttttt ccaccgccgt
    40801 ttacgctgcc tactaatcgt cgatctaaaa gtgggcaaat tcagctatag cgatgccgga
    40861 cagatgaata tgtatctcaa ctacgccaaa gagcactgga cgctaccgga tgaaaatccg
    40921 cccatcggtc tggttctctg tgcagagaaa ggagccggag aagcacatta tgctctggca
    40981 ggtttgccta acaccgttct ggcaagcgaa tataagatgc aactacctga tgagaaacga
    41041 ctcgcagatg aactcgttcg aacacaggcg gtgctagagg aaggctatag acgccgttaa
    41101 tttcagcggt ttcgaattct gcagacagtg tctgcagaat ctttaaaacg aactaagttt
    41161 taagccatta aatcagcctt aaccttcacc acaacctttt taatctctcc aggctcgccg
    41221 acaacgcatc ctggtttatg cggttcacct ggcataaaca cggcgaacat tcccggtttt
    41281 aagatgatgg cttgctcgtt atcaatggtg ctgcaaagct gataatcatc ctcatggtgg
    41341 aactcttcac actggcgcgc agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt
    41401 aataacagtt ggatatcaat gtattgctcg tgcaattccg cttttttctc gacgggcgat
    41461 tgagtgttaa acgtcatgac attcataaag atattgtcgc cctgtaattc gtaacgaccc
    41521 ggcgcttttt cttgcggtct ggcagctaat gccagcgtta acgcgtcctg taacgcagga
    41581 tgtaacccag cagacggtaa tgactgtact tcacccatca tcataatttt tctccctggg
    41641 ccaacagcgc agccccaagt aaacctgcat cgtggcggta gtgcgccgcc agtaaatcaa
    41701 catgaaatgt cgctggctcc tgcgccagat acgtttccac cagcgccaga tacccttctg
    41761 ccagcccaac gctgccaccg accaccacgc actggcaatc agttgtggct ttaatatcag
    41821 cgatcagcct tgcaagcgta cgtgcggagc ggtgaatcag ctgctgcgcc tgctcgtcgc
    41881 cctgcccggc gcgtgtgaaa atagttctcg catccgcgcc cgccagttca ccctgcgctg
    41941 ccgccgcaat gccgcgacca gaagcaatcg cctccacgca acctgtgcgt ccacagccac
    42001 agaccgggcc gtgtggatcg gcaagcgtat gcccgatgtg ccccgccaga ccgccagggc
    42061 cggtaagcag tttgccgccg ctcactacac cgccgccgac gccagtggaa acggtgataa
    42121 agaccatatc ggttatatcg ccttccagcg cctgatactc tgcccatgct gcggcctgcg
    42181 cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc cagcgttttg actaacggaa
    42241 agtgtagcaa tccaccgaga ttatgcggat tcagcgccag caaactgccg tcgcgaataa
    42301 ttccggttga agcgatggca acccgctgcg catgagcttg caacggagag actaatgcgg
    42361 ataaggcatc acgcaaggct tctggtgtct ggctggctgg cgtaggaagt tcacgacgat
    42421 cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa
    42481 tcgccagcgt ggtcatagca ccgccttttt catcgctgtg ttgtaccact gacaaatgtg
    42541 ttcaagacgc gtaatggcag aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc
    42601 tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc accctacatc cggcgtcgct
    42661 caacgttttc accagcgcca gatccggctc ttctggcgtt tcaggcgtgg tatagccaga
    42721 aagcgtagtg ccaataattt cggctcccag cttttggcat gccaggccgt cttccggcgt
    42781 tgagcagtcg gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac
    42841 aggcaccgga cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc
    42901 cagcgcatca acatcttcaa tataggccgt gatgcgtacc ggagaatcct ccagatcgcg
    42961 tttcacaatt ccaataatcg gcacgctcac caccgcacgc gtggcttgca gatttgccac
    43021 accttcaatg cgaatggcaa ccgcgcccgc ctgttctgcc gctaatgcca tggcggcgac
    43081 gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg caggagacaa tcaggccacc
    43141 gttagcagcg attttttgat ccagttgtgc aagtaacgac atacatcttc ccttagcgaa
    43201 aggcccggta catagaccgg gcaacaggat taacttttgg ttttgactaa atcgtttttg
    43261 gcgctgccaa atggcacggc accgctaaat ggtttgccgt cgatagcgtc atgagtacgc
    43321 aatgcttccg ggcgcagcca acgctgaacg cgggaaggca tatccagacc aatcagcagg
    43381 atcaccacaa atgtcagact gaacgatagc gatgccagcg cagtacccag atccagacgt
    43441 tgagcgatca acgcgccgat gattggggcc agtgcgccgc ccaatgcacc aacgttgtag
    43501 gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat cagtttcggt
    43561 aagatcccgg cgatcccttg tccaagcatt tgctggaaga acagtaacag accgagcacc
    43621 cagacgtttg cgccgccaat cgcaaatacc ggaataatca acagctgcga ggccaacagg
    43681 ctacaaacgt acgctttgcg ggttcccagc cagtcaccga ggaagccgcc gacacagcac
    43741 cccaccgcag ccccaaagcc gctaaagaac agcacattgg cgacagtatg cgggttataa
    43801 gccagatcgg ttttcagata cgttggcagc agcgcctgaa tcggccatga gtagaggaaa
    43861 gcaaacaaca cgaccaccat cagcattacg cccgttggcc agcgtttacc cgtactttgc
    43921 accataaagc tgataaagat tgcggcgcat aacagcccaa gaacagcgac gatcgcggca
    43981 ttttgcaggt taccggcgaa gcaaaaccac agcgcagtag ccgccgccag tgtcattacg
    44041 atattggcaa tgcgatgttc accacggtag agaatatcta ccatcgtgcg tactggtgct
    44101 ttacctgcat gtttctcttt ccagtcttcc gcttccggga tgtttttacg cagccagaga
    44161 gcaaagatga taggcaaaat gccgataaag aacagcgcac gccagcccca gaccggaacc
    44221 accaggctat agacctgagc ggcaacgacg gcccccacag agaagcctga aatcagaaaa
    44281 ccactggctt tgttacgcag atgttttggc cagctttcaa tgacataggt ggcgctggaa
    44341 ccgtattcac ccgccatccc catgccgatg accagacgcg cgataaacat ggtgatgtag
    44401 cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt
    44461 gccagacgac gcccgtagcg gtcacccata gcgccgagca tcaggccgcc gaaccagcga
    44521 gagataaagg ctgcggagat cagacttgcc gcctgcaccg tcgtcagccc gaattcacct
    44581 tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc
    44641 aaccaggcag cggaaaatgc ccgccattgt gcacgattga gatggcgata ccacgggata
    44701 ttctgggttg tagtactcat tgtgagtctc ccgcggtggg cgatgcccac acgctttggt
    44761 atgaaaattg tagggtacag atgcgtttat ttcccctcac ccggtagggg cgagcgaggg
    44821 gaaaacaact cacccgcgct cttgcatcaa ctgctgggcc agcgccttca gttctggcag
    44881 atatttttca tctaccggtc caaacggttt gcggcacagc ggcacagaaa cgacatccat
    44941 ataatggagg acagttttca ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac
    45001 tttattgcat tcagtttgca gtttctgcgc ggtctggata tcgccttctt tcagcgcctt
    45061 aacgatcccc tgatagcgcc agcccatgat gttgtaggta ctaccgatac caccatcagc
    45121 gcccgtcagc agaccagagg cgaagatttc gtcgtaaccg ttatagagca caagatcagg
    45181 atgttcacga cggatctgct ccatctgata gagatcgcca gaagtctgtt tcagcgcacc
    45241 gacgccaggc aacgtaacca gggtgttgat ctgatccagg gtcagtttta ccccactcag
    45301 ggctggaatg ttgtacacca ccatcggcaa accatccgct gaatcaataa ttgcccgata
    45361 gtgatcgcag tgttcttcaa agctgaaagg atagtagaac ggcgtgacgg cggagacggc
    45421 atcgaagcca taacgtttag ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca
    45481 accgacgtgg gcgatgagtt taatcttacc tttcgcctct tcggcgacga tttccagtac
    45541 ctgttcacgc tcggaaaggc tttgtacaaa ggcctcgccg gtcgaaccac ccacgtataa
    45601 accgtcgatg ccctgctgaa tattgaactg aaccaggcga cgcagactcg ctttatccag
    45661 tgcttgttgt tgatcaaaag gagtcaggag tgcagccatt acgccacgta aattcgttgc
    45721 cataaatacc tctgaagtga tgcttgtctg ataaacgata tacctttata cctgttatac
    45781 cagatcaatt aagcaacacc ccatacagaa agcttataat gcgatctgct tcactaaagt
    45841 ggcattattt ctttttgttg gtggtctgac cgaaagcgtg ccaggtagca gagacgctgt
    45901 tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa
    45961 cgatcgcaat atgctgttga taactaacgt tgttatgttc gtgcagtgcc tgatcggtaa
    46021 ccgttgggcg tgcggcaata agccagtcga gcagggcaac gtggatcgcc atgaagattg
    46081 ggttaccggg gatctccgcc agcacgcggt ggaaatcaac gtctgaacga atgaatgccg
    46141 cgttgttatc cagcgactga ctgttaattt ccagtgcttt tgccagcaaa tcgatttgct
    46201 catcggtggc atgttcagcc gcatagcgca ccaggctgga ttcaaagaac agacgtaatt
    46261 gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc tttcgccatg ccggaaagct
    46321 cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc acgttcgccg ttgttgattt
    46381 gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg cacagaagga cgcccgacgt
    46441 taaagaacgc catcagttcg cgttcagacg gtaattgttc accttcgcca aattcacgac
    46501 ggcggatcat ctgttccagc tcttcttcca ccatttcgga gagtttttta cgcgccagcg
    46561 ggcggctacg caagttgcga ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg
    46621 cgttcataag gcccattctg taaggtcagt gtgattaaca tcatcagtga catcctatca
    46681 caggattgaa agtaggggaa aatggcaggt ttttctcttt gtgcctcatc attaccataa
    46741 ttaacggaat aattaactat tgcgaaaaat taatgtaacg cagataaaaa catcccgttt
    46801 gaattattta taagactatt cacgagcatt atgaatatta tgaatgtgtt cttacaaaat
    46861 aatcataagc gcatattttt taatgaaaaa tcacctcacc tacaattaaa aacacgacat
    46921 ccgcaccata aatagacttg caaaaaatat aacatcattg ttttcaatct gccgtttatg
    46981 ggattgaccg ttttcttttg acacggagtt caacaatgtt cggcataatt atatctgtca
    47041 tcgtattaat tacgatgggc tatttgatcc tgaaaaacta caaacctcag gtggtgctgg
    47101 ctgccgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttcggt ggtgtactcg
    47161 atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc ctgcgcatgc
    47221 tgtccaaccg cattgccgga ttggggctgt cgattatggc ggtgggcggt tatgcccgct
    47281 acatggagcg cacaggagcc agtcgcgcga tggtgagctt gctaagccgc ccgttaaaac
    47341 tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggccaa atcatggcgc
    47401 agtttattac cagcgcctcc ggcctgggta tgttgctgat ggtcacctta tttccgacgc
    47461 tggtgagtct gggagtaagt cgcctctctg cggtggcggt tatcgcaacc acgatgtcca
    47521 ttgagtgggg gattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa
    47581 ttgccactta cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg
    47641 cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg
    47701 aacaggcaga gctaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg
    47761 tgatgccgtt aatcttgatg ctcggctcgc tgttcctcgc ccacatcggg ctgatgcagt
    47821 cagaactgca tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt
    47881 tcttccgcaa gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca
    47941 tgagtacgca gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag
    48001 gcttaacgac gattggcact gttgatgcgg ttatcagggg tgcggagcat tctggtctgg
    48061 gcggtattgg cgtgatgatt attatggcgc tagtcattgc catttgtgcc attgtgatgg
    48121 gctctggcaa tgcgccgttt atgtcatttg ccagtcttat tccgaatatc gcagccggac
    48181 tacatgtacc agcggttgta atgattatgc cgatgcattt tgccacgacg ctagcgcgcg
    48241 cggtttcgcc gattactgcg gtggtggtcg ttacgtcagg aattgcaggc gtttcgcctt
    48301 ttgcggtggt gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg
    48361 ccacaatcac gctattttat taagccatta aaagacaaaa caggccacca gggcctgttt
    48421 ttgtattact tcacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca
    48481 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
    48541 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca
    48601 gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca
    48661 gccagcggca cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa gcgtacctca
    48721 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
    48781 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaagtgc
    48841 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
    48901 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
    48961 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
    49021 gctctttcgc acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg
    49081 gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga
    49141 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
    49201 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
    49261 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
    49321 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
    49381 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
    49441 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
    49501 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
    49561 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
    49621 agtcagaagt tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa
    49681 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
    49741 tttgtggtga gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
    49801 gacttagtcc cttattggcg atgtggtttt tgttttgcct gtctgtcaga cggtagcaaa
    49861 aagcaacttt ccaattttta cgctgattca gaatttaacc ataaaaaaac ccgccgaagc
    49921 gggttttttc gcaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
    49981 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
    50041 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
    50101 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
    50161 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
    50221 cgagcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
    50281 ttgcccggtt tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat
    50341 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
    50401 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
    50461 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
    50521 atcatctctt caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
    50581 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
    50641 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga
    50701 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
    50761 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
    50821 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
    50881 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
    50941 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
    51001 tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
    51061 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa
    51121 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
    51181 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
    51241 caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
    51301 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
    51361 ccaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcgcc cccgccagcg
    51421 ccaaccatag tttatccatc tgcgcccggg tttcatcgtt gagtggcgaa agccacagat
    51481 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
    51541 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcagg ccatttcgat
    51601 aaagttcatc cggcggaata tttgacgtcg ctaccagggt aatgccgcgg gcgaacaggg
    51661 ctttcatcag accgccaagt agcatggcgt cggtaatatc agaaacaaaa aattcgtcaa
    51721 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
    51781 gcccctgtaa ggcagttagc tcttcgtgca cccgcagcat aaaacggtga aagtgcaggc
    51841 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
    51901 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
    51961 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcctgc
    52021 tattgatgag ttcttgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
    52081 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
    52141 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
    52201 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt
    52261 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
    52321 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
    52381 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
    52441 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
    52501 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
    52561 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
    52621 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
    52681 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
    52741 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
    52801 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
    52861 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca aacccagctg ttgagtgcat
    52921 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
    52981 gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc
    53041 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
    53101 aattcaaaaa gttttttggt gatgatttac cggatcaacc tgcacaaccc ttcgaaggtt
    53161 taggttccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
    53221 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
    53281 tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat
    53341 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg
    53401 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggcc
    53461 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
    53521 gcggtaactc cggcggtgca ctattaaacc ttaacggtga gttaattggc atcaacactg
    53581 caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg
    53641 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
    53701 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
    53761 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc aaaagcgggc gtcaaagcgg
    53821 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
    53881 gtatcgcgac tacagagccg ggcacgaaag tgaagcttgg cctgctgcgt aacggcaaac
    53941 cactggaagt agaagtgacg cttgatacca gcacctcttc gtcggccagc gctgaaatga
    54001 tcacgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
    54061 gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
    54121 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
    54181 tgctggcggc aaaaccggcc atcatcgccc tgcaaattgt acgcggtaat gaaagcatct
    54241 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa
    54301 ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
    54361 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
    54421 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
    54481 cagttataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
    54541 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
    54601 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
    54661 cgtcgcctta caggatggac gtgtatttga agcattgctg gtgggatctg actctctaac
    54721 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc cgatcaatgc
    54781 acgtcgcgta ccgcacattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
    54841 gcagaccatt acccagggga ttattagtgc tacgggtcga atcggtctga acccgaccgg
    54901 gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
    54961 ggtgaactcg ctgggagaac tgatgggcat taacacgctg tcgtttgata agagtaacga
    55021 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
    55081 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggtatcg gcggacgcga
    55141 gatcgcacca ctgcacgcgc agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
    55201 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
    55261 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcagaaat
    55321 tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
    55381 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
    55441 agtctgtgct ccggtttttt attattcgct aattaattac ttattaacga actcttcgcc
    55501 cagggcgata tctttcttca gcgtatccag cataccttcc agtgcgctct gttcaaatgc
    55561 gctcagggta ccgatagatt tacgctcttc cacgccgttt ttacccagca gcagcggttg
    55621 agagaagaaa cgggcgtact gaccgtcgcc ttcaacgtag gcacattcga caacgccttg
    55681 ttcgccctgc agtgcacgaa ccagagacag accaaaacgt gcagctgcct ggcccataga
    55741 cagggttgca gacccaccac cggctttcgc ttcaaccact tcagtacctg cgttctggat
    55801 acgtttggtc agatcagcca cttcctgctc ggtaaaacta acgccaggaa cctgtgacag
    55861 cagcggcaga atggtaacac cagagtgacc gccaataacc ggcacttcaa cttcgcctgg
    55921 ctgtttgcct ttcagttccg caacaaaagt gttggaacga atgatatcca gcgtggtaac
    55981 gccgaacagt ttgtttttgt cataaacacc ggcttttttc agcacttcag cagcaatcgc
    56041 aactgtggtg ttaaccgggt tagtgataat accaatgcac gctttcgggc aggttttcgc
    56101 aacttgctgt accaggtttt tcacgatgcc ggcgttaacg ttaaacaggt cggaacgatc
    56161 catacccggt ttacgcgcta cacctgcaga gataagaacg acatctgcgc cttccagcgc
    56221 cggagtcgca tcttcaccag aaaaaccttt gattttcaca gcagtaggga tatggctcag
    56281 atcgacagcc acaccgggag tcactggagc gatatcatac agagagagtt ctgaacctga
    56341 aggcagttgg gtttttaaca gtagtgcaag cgcctggcca ataccgccag cagcgccgag
    56401 gactgcgact ttcatcctaa actccttatt atattgataa actaagatat gttgctccgc
    56461 tgccgcgacc ttaatccaca aaattgccgt ttacaatgac cacatctcaa gaatgtgtag
    56521 tcacgcaagt ttagcgttta tgcatttaat tgccgtaatc aggaacctaa cttacgtaat
    56581 taacagtcgc ttatgcatta gcgcaacatt ccaacaggtg gtgacaatat accctaccgt
    56641 tcagccaaaa caacatcaat ttgataacaa ttaatttact tttaaacaga atttgcatgc
    56701 cgtgacgcag gcatgtttct caataacgaa atttgataaa atcccgctct ttcataacat
    56761 tatttcagcc ttcttcaggg ctgactgttt gcataaaaat tcatctgtat gcacaataat
    56821 gttgtatcaa ccaccatatc gggtgactta tgcgaagctc ggctaagcaa gaagaactag
    56881 ttaaagcatt taaagcatta cttaaagaag agaaatttag ctcccagggc gaaatcgtcg
    56941 ccgcgttgca ggagcaaggc tttgacaata ttaatcagtc taaagtctcg cggatgttga
    57001 ccaagtttgg tgctgtacgt acacgcaatg ccaaaatgga aatggtttac tgcctgccag
    57061 ctgaactggg tgtaccaact acctccagtc cattgaagaa tctggtgctg gatatcgact
    57121 acaacgatgc agttgtcgtg attcatacca gccctggcgc ggcgcagtta attgctcgcc
    57181 tgctggactc actgggcaaa gcagaaggta ttctgggcac catcgctggc gatgacacca
    57241 tctttaccac ccctgctaac ggtttcaccg tcaaagacct gtacgaagca attttagagc
    57301 tgttcgacca ggagctttaa tctctgcccc gtcgcttctg gcgtcgggga aaatgttgct
    57361 tatccctctc attccccttc tttctcctgc gatacattta acgaatagtg cgttttaccg
    57421 cgacacatca ttcacaaaat gaatacatac ggtaaaaaaa gcacattatg caaaattcat
    57481 tatctaattg aaaatactat aattaataat aaataaccgt gttttttata cttttttgtt
    57541 attaaaattc acatttttaa cacttagtat caactgaaac agttagcgtg gtattaatta
    57601 gttcaataat tagtgtatac ttgattttgt gatatgggtc acaaaacaaa ggcccagcta
    57661 aaagattatg tcgaggtaaa aatcatgaaa atcaaaacca ctgtagctgc attaagcgta
    57721 ctttctgttc tctctttcgg tgcattcgct gccgactcca ttgatgctgc acaagcacaa
    57781 aatcgtgaag caatcgggac cgtatccgta agtggtgtgg cgtcttcgcc aatggatatg
    57841 cgtgaaatgc tgaacaaaaa agcggaagag aaaggcgcaa cggcctacca gattactgaa
    57901 gctcgtagcg gtgacacctg gcacgctacg gctgaactgt acaaataaac cctcatcgtc
    57961 ttgtccgacg atattgcccc cggttcgggg gctttttttt gcgctaatga cgaacattaa
    58021 aacgcaaatg cccttccagc tcttcctctg cctcatcaaa cagcaatatt aacgcgccaa
    58081 aacggcgacg cgttttctct cccagatgaa caaattcaat ctcaagtggt agcggcagga
    58141 catcgttcat taacacatcc catagtgagt cgagatcgcg taccttatct ttagccagac
    58201 caaaggtttg gctaaagtca cgataaaaat cctcctgact ctcaatctca tcaaaatcaa
    58261 aggtataaat attcatctgt tgccaccgtc acgtttcgcg gcgagttctg ccgtgcatcg
    58321 ctaagtatag ccatgaaaaa accgacgctt ttggcgtcgg ttttgactta actatcggtc
    58381 aacgcatgtt gatacttatg gagcatcccc gccagccgat gaaccggttc cgtcacctgc
    58441 ggtggcgctt gccagatgcg tagcttttcc tggtagattt ccagttcttc cagcaactgg
    58501 ccaaagtacc gacgacgttt atcatcgcta cgggcagata tcacatggtc tgctgtgcgg
    58561 cgcatttgtc ggtgaaacgc cgataaatcc tcgttaaccg ggatcggcgc atcacgcagg
    58621 cgctggtgcg cgataatcat cgtcagcgcc aggcgaaatt tcggcaaatc ccctgggaac
    58681 ttattcatca gcaaaaacag ctgctgataa agtgccggga ggtggttctc tttacgacgt
    58741 gccacattgg tagtcatcgc ggaaacagcg gcagaaacaa actgattaag cagtacacgt
    58801 ccggtcctgt cgcgcgattt atcccgcaca agcaaaataa cggtgaacgc gagcacacag
    58861 ccgacgattt gccctaatgc gctgtcgaga aactgactga aatggaaagt catcgggtta
    58921 tccagcacga taatatttat ggtgctggcc agcgccccca tcgagcccag tcgccgtttc
    58981 tgtacttcta taccgaggaa aaatcccagc actgccaggc taatacacag cagcaacatg
    59041 ctctgttggg tattagggat aatcaccaaa aagtagagca gccctaacgg cagcgcggcc
    59101 agcgtcccgt agataaagtc gatcgccacc atgcgtggat tcggcaaacg cattgccagt
    59161 gacgtcacga ccgcaatcat caccattgca ccactgccgg aagtccagcc cgtccacagc
    59221 cagaaaagcg tgcccagaat gcaggaaagt gtggttcgcc agaagttaac catcgcatga
    59281 tgacgttcgg ctgactctac tttgacttcc ggttcgcctt gcaggatctc ttcttcggtg
    59341 gcgttgattt ttgtgttact gataacgccg cgcttgagaa gctgataacg cgttgccgcc
    59401 gcgacccagc tataaatggt gacaggcgtt tcccgttccc cggtccaggc gataactctc
    59461 cgcaggcgtt tgagctgctt gtggacgtcc tgcgcgattt ctaccggcgt gtcaaaaaat
    59521 tcgcggaaag tatcagtgat caattccggg cgcgtattct gaataagata agtttcgcag
    59581 gattgggtaa tcagcgtcag cgatagcgta ttgatcgctt ttaaacgtcg attggcccgc
    59641 gcccagcggg aagattccat attcaggttg ctgcgcatgc cttgtagcgc cgtggtgcgt
    59701 cgcaccaggt cgccccaggc tttatcgaca acttcaccat cgccatgctt gatacagagt
    59761 tgcattaatt gatattgcgc gaccagcaaa ctttccagct ctcgatccac ttcttgtttg
    59821 atcgatcgcg gagaaaagag caaatccgcc ataatcgcac acacaatacc gataacgatc
    59881 tcgctacaac gttcgacggc aaactgcggc gtaagcaatg gttccggctg aatggtgatc
    59941 acaatgatca gcgcggtata accggccagc ccccacgcgt acgagttttc tattcgtacc
    60001 agcgaggata tccaggtaca aaaaccggcc cagatacagc acaccagaat catcaataat
    60061 ggtgcgcgga tcatcgcaat gatgatcacc agtccggcaa tacagccaat aaatgtgccg
    60121 atgatgcgca aaaagccacg atagcgaata gcgccagaat acggttcacc tcccgcagca
    60181 aaggccggac cggcggcaac aatcgccgct gtcagtaccg cccagcgtgg cgtttccagc
    60241 tggaagtgaa agccaacaaa cagcgccagt acaatggcgg tcgccagttt taccgcaaag
    60301 cgaatatgtt ggttagcaat ggagaaaata cccatcgtga ttaaccaaac tcacgcaggc
    60361 gatgggccat tttacggaag aacgaatcct ggctttcgtc gcgatcttgt ttgccagtga
    60421 ccaccactgt agcagtggtg cccgcaggcc agatgttctc ttgctggttg tcgagacgaa
    60481 tacgaaccgg aacacgttgc gccaaacgca cccattcaag gttagagtcg atggtcgcca
    60541 tccctttgtc gtcacgcgtg ctgctggcgt tggtgacccc agcggcaaca ctatcaacag
    60601 tccctttcag cactttgtta ctgccaagcg gcgtgatctc tgcacgatac cccggacgca
    60661 ccccttccag cttagtttct tccatatagg ccagtacata gaaggagttc tgtttcacca
    60721 gcgcaaccgc cgttgatcct cgagtaataa actcaccggt atagacgttg aggttggtca
    60781 cccagccatc tgctggcgcg cggatcaccg tgcgttcaag atccagtttt gccagatcgc
    60841 gggtcgcctg cgctttcgct aactgatgca gaaccgtttg tagtacgttg ttagcctggt
    60901 cgatctcttc gcgagacatc gcctgcacac cgagacggtt acgacgtccg gcctcctggc
    60961 gtttctcctg tgccagtacc tgataataag caacatcggc ttgcgcttcc tcaagcgcct
    61021 tttgatagcg cggctggtcg atggtgaaca gtatctgtcc ttttttcacc agctggttat
    61081 catgaacatt cacctgggta atgagtccag aaacgtccgg cgcgatcgca acgacgtcag
    61141 cgctaaagcg cgcgtcacgc gtccaggggg attcggtgta atagacccag gcattaaaaa
    61201 ttgcgatgaa ggccagaatg actaatacga ccgtgatggc cgtacgggag aattttctta
    61261 ttagtgtttt cacttcaacc tcaaacgaac agtcgcgata tcaaataaaa caagcagcaa
    61321 tagagcgcgg tgttgaacaa cgccggatgc cagacaaagt cgtagatacc tgttggcaca
    61381 agtacccggc gcaccagcca gaaaatcgcc agtgataaaa gcaattcaaa aaatatcggt
    61441 gggaaggaca gcccaaacac cacgataacg ggaaacagac tcatgttgac cttggttgta
    61501 tagagagagc aggcgttatt attttcagca tctgtcgccg cagagaaggg catggaaagc
    61561 cgggcgagag caacattgct gtagattgat atttaatata ttagcgtaac tgttatgctg
    61621 ttatctatat tatgtgatct aaatcacttt taagtcagag tgaataatgg aacggctaaa
    61681 acgcatgtcg gtgtttgcca aagtagttga atttggctct tttaccgccg ccgccagaca
    61741 gctacagatg agcgtttcgt ccatcagtca gacggtgtca aaactggaag atgagttgca
    61801 ggtaaagctg ttaaaccgta gcacacgcag cattggcctg accgaagccg gtagaattta
    61861 ctaccagggc tgccgtcgta tgcttcatga agtgcaggat gttcatgagc aactgtatgc
    61921 cttcaataac acccccatcg ggacgctacg cattggctgt tcttcaacta tggcacaaaa
    61981 tgttctcgcc gggctgacag ccaaaatgct gaaagaatac ccaggtttga gcgtcaatct
    62041 ggttaccgga attccagccc ccgacctgat tgccgacggt ctggatgtgg tgatccgcgt
    62101 cggcgcgttg caggattcca gcctgttttc ccgccgtctt ggcgcgatgc caatggtggt
    62161 gtgcgccgcg aaaagctatc tcacacaata cggcataccg gaaaaacccg ccgatttaag
    62221 tagccattca tggcttgaat acagcgtgcg gcccgacaat gaatttgaac tgattgcacc
    62281 ggaagggatc tcgactcgcc tgatcccgca agggagattt gtgactaatg atccgatgac
    62341 gctggtgcgc tggctaacgg cgggtgccgg gatcgcctac gtgccgctga tgtgggtgat
    62401 caacgagatc aatcgcggtg agctggagat cctgctgccg cgttatcagt cagatccacg
    62461 cccggtttat gcgttatata ccgaaaaaga taagctgccg ctgaaggtac aggtcgtgat
    62521 caactcgctg acggattatt ttgttgaggt cggtaaattg tttcaggaga tgcacgggcg
    62581 cgggaaagag aagtaattca tattgtactg ttacgttcta caaacctgtg ccaacgggtt
    62641 cccctcaccc taaccccaga ggagcgaggg gaccgttcgt gcacgtagaa agattaatta
    62701 tccttctgaa aataatgaaa ttattacgca gtaccgccaa cagtcaggtt atcaactttc
    62761 agcgttggct ggcccacgcc aaccggcaaa ctttgccctt ctttaccgca gacacccacg
    62821 ccgttatcca gtttcaggtc gttgccaacc atcgaaatct gctgcatggt ttcgataccg
    62881 gaaccaatca acgtcgcgcc tttcaccggc ttcgttactt taccgttttc aatcagatac
    62941 gcttctgaag tggagaaaac gaatttgccg gatgtaatat ccacctgacc gccaccaaag
    63001 ttcggtgcat agataccgta ctcaacggat tcaataattt cctgcggggt cgatttaccc
    63061 ggcagcatat aggtgttggt catacgcggc atgggcagat gggcgtagga ttcacggcga
    63121 ccgttgccag tcggcgtcat ccccatcaaa cgcgcgttga gtttatcctg catgtagcct
    63181 ttcagaatgc cgttctcaat cagcacgttg tactggcctg gcgtaccttc gtcatcaatc
    63241 gccaccgaac cacggcgatc gaccatcgtg ccgtcatcaa ccacggtaca cagttctgaa
    63301 gccaccagct ccccgacctg cccactaaat actgaagtgc cgcggcggtt gaagtcgcct
    63361 tccagaccgt gacctaccgc ttcatgcaac agcacgcccg gccaacctgc gccaagtact
    63421 accggcatgg tgcccgctgg tgcagcaacg gcagaaagat tgaccagcgc catacgcact
    63481 gcttcttttg cccatgcatc cgcacggact tcgccgtcaa gatcggcaag gaagaactca
    63541 taaccaaaac gaccgccgcc gccactggca ccgcgttcgc gtttgccatc ttcttcgacc
    63601 agaacgctca cggaaagacg caccagcgga cgaacatccg ccgccagcgt gccgtcggtg
    63661 gccgcaacca gaattaattc ataaacgccg ctgaggctgg cagtcacttc ttgtacgcgc
    63721 ttgtccgctt cgcgggcaac cttatcgacg cgacgcagga tatccagctt ctcttcacgg
    63781 ctcatgcttt gcagcggatc taccgaggta tacaacgggc tatgctctac cgcgcccagc
    63841 gtctgtactt tgccatcacc actatcacgg acgatggtgc gcgccgcttg cgcactctgt
    63901 tccagcgcca gcaggctgat ttggtcagcg taagcaaatc cggttttttc accactgatt
    63961 gcacgcacac caacgccctg atcgatgttg taagaaccat ctttaataat gcggtcttct
    64021 aaaacccagg attcgtgata gctcgactga aaatagagat cgccataatc aaggcgacgt
    64081 tcggccagtt gaccgaggat cgcgaacaag tcctgatgtt tcaggccgtt cgccgctagc
    64141 aattgttcac ttaccaggtt cagactcatc gtttttgcta ctcgttagtt actgcagtag
    64201 aggatttttt acggctgccg gatgcggcgt gaacgcccta tccggcctac agttatgttc
    64261 cggctcgttg gcctttggca acgattatca tatgagattg gggcaattac gcgccctcgt
    64321 caaatcattg cgctttttct ttacgcggtt ggcgcaacac ttcgttgatt tgcggatcgt
    64381 ccagcggacc cgaaatgtga tagcgcaaaa tggagacttt gctccacagc ggccccagca
    64441 ctttactggc tgcaaatact gccgcgccaa caatgaggtt aaccgcaaaa gccgcagcca
    64501 cgcccaccgt cgcagaaatc tctggtgcga caaccgcttc catattcagg tcgcgacgta
    64561 ccagatttac cgaccctttc atggcgatat ccgcctccag gccatccacc agcgtgtcgt
    64621 cggtgtgcat aaccccgtct ttaatccacg cggtgctgcg aatggagtca aaatagaacc
    64681 cttcgccaaa agtgtctctg aaatcaaaac gcagcttacg catcagggca tcaacgctca
    64741 ataagcgcag caattgcccg gcatgtccgg tattgatttc ggtaatttcg cctttaccca
    64801 gttgagtatg aatgatgcca ttcaacgtcg cctcatctgg ttgccagggt gctttgcgcc
    64861 agtgtaaatc gtaatccaca ttaaatgacg actggcgtat gggcgtcgtg acaccaaaaa
    64921 attctgcggc ggcatcaatt ttctgcccgc gcagttttcc tttcagcgag gtacgttcat
    64981 tctccggatt attaacccat tcaccatcgg cagtaagccg cgagaaacca gtatcaatca
    65041 gtccattggt cagcgttaac gtatcgccag aaatggtgat atcactgtca atgcgaccga
    65101 atttttgccc ccagaaccag cactctgtgc atcgtatttg tgcgtccggc cagccacgga
    65161 agttaatgcg ctccgttgtc gggaaaggtg atgacggcgt tgaatcacca cgagttttcg
    65221 ccacgctcgg gttgtaataa agatatttga tattcgccag ccacggcgcg ttattacgca
    65281 tcgctagcgt ggcgttgatt tcacgccctt gcgcctcaac cagggtgcca tttgccgtcg
    65341 gttgcgaaac aatactcagg ttattccatt gctgatttcc cagtgacaac ataggcgtac
    65401 gtaacgttat gtgttgtggg aaactcgctg caccaccgac actctccgca gcgcctttct
    65461 ggaacagggc cagccactcg gcaccattca tcggcggcat attgagttca acgccacttt
    65521 gttccggcaa cggcgggagc gttttactgt ctgccgccca aatagcacga tcgagcgtca
    65581 gcttttgacc aagcaaccag cggctattga aatggttatc cgcaccagcc tgtccggtta
    65641 attcaaagct gttgagattg ccatcaacct taacgtttac cgctagtggt tcacccgcag
    65701 gtttggctaa cggtgaaggt aagtgactac tcacattatt cagatcgccg ttcagctcta
    65761 cgttataggt cgcgccagca tgataaggca gatcaatgcc cactttacca tcccacgcca
    65821 cgctgccact caatgcttcg ttcaccgctt caggcagaac gctggttttc gccggttgcc
    65881 agttaccgtt gagattcacc gcaacctggt aggcttttgc cccttctttg gtggaaaaat
    65941 ccacgttcaa cggctgatta aaccagcttg ctgtcagtgg ttcactttgc agatcgccat
    66001 tgataaagct gaatttaccg ctcaaatttt tcagggtgct gtcgagtggt ttgataaaca
    66061 gactgttatt acgcagcgtc acttcacctt tcgcggttac cagttcgccg ttcagcggga
    66121 tatcaagatg taagcgagca ttcacatcgc cgtcgagctg gagttcttgc agggtcgcac
    66181 ccagagaatc tttcagcggt gtctcatcaa agtaagggcc aacggcttta cccggacctt
    66241 taatgtcagc gtcaatcagc agtttttctt tcgagtagtc agggatcact gcggtgagat
    66301 tactcgcgcg cacgccgccc agattaacgc catcggtttt catccataaa ccgtcgttaa
    66361 taaagtccag ttcaatatca aggttagtta atgcaggcca gtccggctgg aaggcaaact
    66421 tcgcgttgcg cagcggcacc agcacttcaa actgaccttc gttgtgttta taggggaaga
    66481 gttgcggatt gccaccataa accagcgtcg cgttatccgc ttcaccgccc tgaatcgcgc
    66541 cacttaagta atcaaccagg tctttaccca tcaagttttc cgggaaatag cgccaggctt
    66601 gtgaaccatc atcggtactg atgccagcca gaatacccag ccagggttca tcgttagcag
    66661 gttgcaggta acgaaaaccg ccgcgcgcat ggacggcttt ggctttaacg tcaatattac
    66721 gcccatccag ctggaaacct ttgtcattgt tcagccagct tatagttgcc tggccgtcgg
    66781 cgatttccag tggcgcacgg aataccgttt cgtaaggcat ctttgcctgc ttcatcgacg
    66841 cggtaagcaa accattttca acgctgccgg aaagcgtccc ggagaagtgt tccgcacccg
    66901 gtaataattt ccattgcttc caggccagat cgctccacga tgcctgaaaa cgggtcttgt
    66961 ctgccgcctg aagcgggata tccagcgcca gagtgttaat cttgccgctc ggttgtgtgg
    67021 agcgccaaac atcacccagt gcaggtgaaa gtttcgcggc cagcgggcgt atgccctcca
    67081 ggcctgccag ctccagatta ctggcgcgaa tccggagttc gtcactgcgt ttattgtctt
    67141 tgccgccaac gtcctgttcc ggtatccagg ccagcgtcaa tgctccgctc ggccagggtt
    67201 tgccgtccat cgtgatccgt gtatcgggaa tagagaactg ccagcccgga ttttcacgcg
    67261 taatatgcgc ggtcagatta tccaccgaca gcgtatgcgt ttgcttctcg cccaaccagc
    67321 tggcaccgcc ctgtttcagc cagacgtcac cgccggttac atcgcctttg tcgatcgtca
    67381 tccagccttc aagggagaac tgtgccgttt ccagagcaat attgtcctgc atccatttac
    67441 cgagccacgg cttcaggtcg atgtcatccg cctgtagcca gacgcgacca ttgcttaaca
    67501 acccctcatc atcgcgcaaa tccatgcgca cctgcatcac gccgtgctgt ccggtaaggc
    67561 tggagaggct taccaggcct tccgcacggt gtcgacgtgg atcgttcagc caggtgagtt
    67621 gtgggatcgc cagctcggcg cgctgaccgg atggcgtcag aaaactgact tcactgtcgc
    67681 ggagatcgaa atggtcaaat tgacgaagaa acagatcgct gatgtgactg gcttccagac
    67741 tgtcatcgct accaccgctg gtgataggag tgttggtgcg aaagcgcagc tgccagaaag
    67801 tgaggtcgcg aaactgccag cgcatatgta acaggctctg ccagacatcc agcgccagag
    67861 taacgcgttt aaccgaaaat tcgccgccat cttttagttc tgcacggatg tcgtgtgctt
    67921 caagcgtcgg gccaaaattc tgccagctgg ctgagagctg actggcttct accggcatgc
    67981 cagtcgcgga ttctattttg ttgaggattt ccggacgcca ggcgtcaaga tgcggtaaag
    68041 caatacgcag gccgctaacc agcagagcag cgatcacaac gagcgcggct ccagtaagca
    68101 gtaaaatccc cggcaatcgc ctcacccgtc actccttgtc tgctaaaaat gtgactcaaa
    68161 aaccctttgc cggatggcgg cccagcatct gtttacatca ttacgacgtc aaactgctcc
    68221 tggttataga gcggttcaat ttgtacttta acctgtttgc caacgaaaat ttccacttcc
    68281 gccagcgagt gtgactcttc gcctttcaag gcttcagcta ctgccggaga tgcatagacc
    68341 aggaaacggt cggagtcgta agcatggtgg acacgaacaa tctcgcgcat gatttcatag
    68401 cataccgttt ccacggtttt caccgttccg cgaccgtggc aggttgggca ttcgttacac
    68461 agtacgtgct caatgctttc gcgggtgcgt ttacgcgtca tctccaccag ccccagcgcc
    68521 gaaaaaccat taacgctggt tttcacccgg tctttgctca acgcctgctc cagcgagtgc
    68581 agcacgcggc ggcggtgatc ttcattattc atatcgatga aatcaataat gataatccca
    68641 cccagattac gcaaccgtaa ctggcgagcg atagcctgcg tcgcttcaat attggtattg
    68701 aaaatggtgt cgtccagatt gcgatgaccg acaaacgctc cggtattgat gtccacggtg
    68761 gtcatcgctt cggtctggtc gataatcaga taaccaccgg atttcagttc tactttgcgt
    68821 tccagcgctc gctggatttc gttttcgaca tcaaagagat cgaaaatcgg ctggcgtccg
    68881 gtgtaatgct ccagcttgct ggtcatctcg ggaatgtact ccgaggtaaa ctccagcaat
    68941 gcttcgtaag tcaggcgtga gtcaacgcga atgcggtcca gttcggcgtc ggcgaaatca
    69001 cgcagaacac gctgcgccag cgccagttcg ccgtacagct gataacgggt ctgcgggcgt
    69061 tttttacgct ccataacttt ggtccagacg cgtttcagat aagcggcatc ggaggccagt
    69121 tcagcctcgc caaccccttc cgctgcggta cggatgataa acccgccctg ctcgtcgcaa
    69181 tactctgcga ccactttttt caggcgttca cgttctgatt cgctttcaat acgttgggaa
    69241 accccaacgt gagaagcccc tggcataaac accagatagc gagaaggcag cgtgatatcg
    69301 gtggtcaggc gcgcaccttt agtgccaagc ggatctttca ccacctgcac catcagatct
    69361 tgcccctgac gaaccagttc cgagatgtcg cgcaccgtga attgcttttg ttcttcaccc
    69421 gccacacatt cggtgtgcgg catgatatcg gatgcatgaa gaaacgcggc tttatccagc
    69481 ccaatatcta caaaagccgc ctgcataccc ggaagtacac gacttacacg acccttgtag
    69541 atattgccta ctattccgcg tcgcgcctca cgttcaatat gaatttcctg cagaataccg
    69601 ccatcaatat acgccactcg cgtttccgaa ggcgttacgt ttactaacaa ttcagccgtc
    69661 atgtttatcc cttttctcac gcagtgcgtt aaaattactt aataattcat acgtttcaac
    69721 cagcggtaag ccgactacgg cgtgatagct gccatttatc ttcctgacaa aacagccacc
    69781 cagcccctga ataccgtatg cacctgcttt atctaacggt tcaccgctgg cgacatagcc
    69841 cgcgatgtct tcgtctgtta acgttctgaa agtcacatcg gtgaccacca ggcaatcgag
    69901 aatgtgctgg ctgtcggcca acgccactgc cgtcatcacc tgatgggtct gacccgataa
    69961 tttgcgcaac atctgtgccg catgctctgc gtcgcgcggt ttctccagca cttctccatt
    70021 caggataacg atagtatccg cacccagcac cgggagatcc tgcgccgttt gcgcgacacc
    70081 cgcctgtgct ttctcgcgcg ccagacgcac aacatactgc tgcgcgctct cctgcggctg
    70141 acgctgctcc tcaatgcccg taacaatacg ttcaaaggtc acgccaagtt gcgcaagtaa
    70201 ctcctgacga cgcggagaac cggaagctaa atacagagaa gtcatagaaa cctttattgc
    70261 actgcaaact gctgacggac tttacgcatc agcaagaaaa tccacggcca gagcacccca
    70321 ttgactacac tactccagaa cacttccggt ctgaaagaga cgttaatcac taaaaactct
    70381 gcccagaaaa caataatatc caccaccagc gaaagcaaca tgacgaccag cgcctgctgc
    70441 cataatgcga ggttgcggaa aagctggtat ttcagcgcca ccaggtaagc aatgatgctc
    70501 atcgccaata cgcgtacgcc aagcgtcgag ccgctgatca gatccagtat ggcacccatc
    70561 acaaaacctg tgcccacatt tacgcgatga ggcaaggcca ggatccaata caacaagatg
    70621 agtaacaccc agtttggccg gaaaacaatc aggttatccg gccagggcat gatttgcagc
    70681 aacagcgcaa tgaggaaaga gagccagatt acccaacgtc cctggctacg atagctcgcc
    70741 actattgccc tcccggcgca cgcgcaggcg gttgagcacc actttgcggc ggcgtagccc
    70801 tttgtggaga gcgattagca gcaggctgtg tgggcgcagc aggcgcagta gctgcatttc
    70861 ctgtcgtcgg ttgctgcgga gtcggctgag cgatccccgt tgccggttca ggtaactttg
    70921 gccccatcgc gtctggcgaa ggcaatacct gcggcatcat ctgcatcaga cgttcattag
    70981 caacacgatg cacctcttcc ggcgtcatcg ggttagcgcc gttacgatcc gcgccccaca
    71041 gcagcagcag ataacgcaaa cgttgcagtc ctgcagtcgg acgcgcctga atcacagtat
    71101 aagcgcgctg ggtatcgagt tttacggaag agacaaccgc gaccggatag ccttccggga
    71161 aacgaccgcc cagaccggag gtcaccagca catcaccaac acgaatatcc gtattcgctg
    71221 gcagatgctc aagctgcaaa tcatccgtac aaccgttacc ggctgcaatt acgcggatat
    71281 cgttgcgcag cacctggatt ggcagcgcgt gggtcgcatc acaaatcagc agcacgcgac
    71341 tggtcagttt agcgacggcc accacctgac caacaacacc tttgtcgctg atgaccggct
    71401 ggccttcata aacgccatta acgctacctt tatcgataac aacttgatcg ctataaggat
    71461 cgttaaccgt ggagataacc tgagtcacca ttttctgctc atcctgacgc agcggggaac
    71521 ccagcagctc gcgcagacgc gcgttctcct gtttgtattg tccaagcatc agcagttcac
    71581 tgtttttcag caacagttcc tgacgtaacg cccggttttc aagttctagt tggtcacgcg
    71641 aggccagcgt ctgcgatacg ccatccagca attcacgagg agcattggaa acaaagtaga
    71701 aaggactgac ggcggtatcc atataagtac ggatttgact gaacgtcccc aggcggctgt
    71761 cggcaataat aatgccgagc gccaccagca ccgccagaat aaggcgaatc tgtagcgacg
    71821 ggccacggct aaaaattggc ttcataagtt atgcgtattc tcgtatcaga ccaggcaggg
    71881 taaacagaca tttcccctgc ctgcatccga ttactcttcg ctgaacaggt cgccgccgtg
    71941 catgtcgatc atttccagcg ctttgccgcc accgcgcgcc acacaggtca gcgggtcttc
    72001 agcaacaacg actggaatgc cggtttcttc cattaacaaa cggtcaaggt tacgcagcag
    72061 tgcgccacca ccggtgagca ccatgccgcg ctcggagatg tcggaagcca gttccggcgg
    72121 gcactgttcc agtgcaacca ttaccgcgct cacaataccg gtcagcggtt cctgcagtgc
    72181 ttcgaggatt tcgttggagt tcagggtaaa accgcgtgga acaccttctg ccaggttacg
    72241 gccacgaact tcgatttcac ggacttcatc gcccgggtaa gccgaaccga tttcgtgctt
    72301 gatacgttct gcggtggctt caccgatcag agaaccgtaa ttacgacgca catagttgat
    72361 gatagcttcg tcgaaacgat caccaccaat gcgcacggaa gaggagtaaa ccacgccgtt
    72421 caaggagata acagcaactt cagtggtacc accaccgata tcaaccacca tagaaccggt
    72481 cgcttcagaa accggcaggc cagcaccaat tgcggcagcc atcggttctt caatcaggaa
    72541 gacttcacgg gcaccagcgc cctgcgcgga ttcacgaatt gcgcggcgtt caacctgggt
    72601 cgcgccaacc ggtacacaaa ccagaacgcg cgggcttgga cgcataaagc tgttgctgtg
    72661 cacttgtttg atgaagtgct ggagcatttt ttcagtcacg aagaagtcgg cgataacgcc
    72721 gtctttcatt gggcgaatgg cagcaatatt gcccggcgta cggcccagca tctgcttcgc
    72781 gtcatgacct actgcagcta cgcttttcgg tgaaccggca cgatcctgac gaatggccac
    72841 cacggaaggc tcattcaata cgatgccttg tccttttaca taaatgaggg tattcgcagt
    72901 acccaggtca atggacaagt cattggaaaa catgccacga aattttttca acatactaag
    72961 ggataatcct gaaagctggg gcggaaaaga aaatccgctt actttaccaa ccacacgcag
    73021 cagcgacaag gcgcaaaaat catctgctac ggtgaaaatt agtgcagttc gtttcctttg
    73081 ttacaaatct ctgcctgagt ccagaaaggc ttaatgcatc agcagcattc ctcgcctgtt
    73141 tgcaaccgcg taaggtcatt catctgcata tgtgctgcaa caatctggcg agcagacaag
    73201 cacactccca tgagacgcag cgcgcgttat tctacgtgaa aacagattaa acggcaggtt
    73261 aaaccgagta tctttgtgaa tattttttca cgttagtatc aagtggctgt gaggacgcga
    73321 aaaaatcccc ttgcccgcct gtaacaccgc gctgaatcag ggtctgccac tcgcttcgcg
    73381 aacgcacacc ggtggcgtaa acttgggtgc tggtcccgga gcaggcttcc accaggcttt
    73441 gaaccagcag ctggttctcc gttcgcttct caatgtttct gaccagcccc ggatggagct
    73501 tgagtaactc aacattaagt tctttgatcc aactggtgct taccagcgtc aaaccagcct
    73561 ggttgacggc tacccgtacc cctaaagcat tcactaaacg aataacaggt tgtaaacgac
    73621 tgatatgttg acctacatcg gcctctgcaa gttcaataat tatgcgtttt cgttgtgatt
    73681 tttcacattg cattaacgta tcgcgcagcc aacgctgaaa acgcgggcga atcagcgact
    73741 caacggtaac ctgaatcgcc agattttcct ctggccagta acgcaatagt ggaataagac
    73801 ggctgatttg cagacggtca tactcttccg ataagccaaa ctgcaagacc atcggcatat
    73861 actccgccga gctaacctct tcattaccat cgaagatgcg gcacatgagt tcgcgatgat
    73921 gaacctgacc ttcgcgagta accgccggtt tttgataaag gcgcgggccg ccgcgactga
    73981 gcatttgctc gataagcgta cgccagcgaa cattaccgcg tcctttttca ggcaacgagt
    74041 catcgtaaat agcccagcta ttgccgccct gcaatcccgc attacgcgtg gcagactctg
    74101 catgttccat tacctgctcg gtatcctgac cactacgcca ggcgcagata ccaatgtgga
    74161 tcatatcgtc gcgatcgagc attttattgt tcggcaaggt atcgacggct ttgattaact
    74221 gaccggcgat gctctctgcc tcttttaacg tccggtgcgg taacagcgca gcaaaatcac
    74281 tgcggtggta acgcgccagc agtgcgccag ggtagcgcat cataaatgtc gacagcagat
    74341 tcgtcagagt gaagaactgt tcttcaacct ggctgtgccc ccaagtatcg ctcaacatat
    74401 tgaaatccgg cagacgaatc atcatcacga tcccgtgggt acctactttc tcctgatctt
    74461 ccagtaacgt tgctaactga ttatcgaaaa agagtcggtt attgaggccg gttttcacgt
    74521 cctgggcggc ataagagcgg atcagcgtat caagacggct gtgttgttcg cgtgcgttct
    74581 gaatttcacg aagcagcgta tctagcgcac tgctggttct gggcggccat tcatagatgg
    74641 ttcccaacac attagagcca cgctcaccgt ttaagatacg agtagcccgg gtttccagca
    74701 attcttgccc ggcaagttgc cgttgtaacc agcgtaccgc caggaagagc ataacaatga
    74761 taaagccaat cgcccccgtg agcggcgcgg tagtcattaa agaatggaaa tagttgccca
    74821 tcggatcctg ataaaccaga cgcagcgaca tccccggatg ctttatcaac ggaacactca
    74881 gttcgcgaaa cagatcgctg gagccaaccg gacgataact accatttctg gccagggtat
    74941 aaacctgttt atcaccatgg agcaggtcta cacgaacgat atctgccgac atcattaatt
    75001 cggtaatttg tggccttaat gtgctgaagt cattcgacac aaggtgggta tcaatcgccg
    75061 tcgccaccgc ctgaacgcga tgactaaact tatactgaat tgcgttgtag aaacttagcg
    75121 aacagcccag caaagtcaca aaaattgtta acccggtgag cagcgtaaca aaggccgaaa
    75181 atttcgtcgt taatctcatc cttgtgttaa ctccgatagt gaggaagcgg gcatactagc
    75241 aaatcagatt tatctcgcaa tttattgcgc ttcatcggct ttgcttttcc attagcgagt
    75301 atagtcttca gaaattattt tccaatccat catgcacatg aggaccactt atgcaggcgt
    75361 tacttttaga acagcaggac ggcaaaactc tcgcatcggt acagacactt gacgaaagtc
    75421 gcctgccgga gggcgatgtc acggttgatg ttcactggtc gagcctgaac tataaagatg
    75481 cgctggcgat taccggtaag ggaaaaatca tccgtaattt tccgatgatt cctgggatcg
    75541 attttgccgg aactgtacgc accagcgaag atccgcgttt tcatgccggt caggaggtgt
    75601 tactcactgg ctggggcgtt ggtgaaaacc actggggtgg gctggcggag caggcgcgag
    75661 tgaaaggtga ctggctggtt gccatgccgc aagggctgga cgcgcgtaaa gcaatgatta
    75721 tcggtactgc cggttttacc gccatgctgt gtgtgatggc gctggaagat gccggtgttc
    75781 gcccgcagga cggggagatt gtcgtgacgg gtgccagtgg tggcgtcggc agtaccgccg
    75841 tggcgctgct gcataagttg ggttatcagg tcgttgccgt ttccggtcgc gaaagtaccc
    75901 atgaatatct gaaaagttta ggtgccagtc gtattctccc tcgtgatgag tttgccgaat
    75961 cccgtcctct ggaaaaacaa gtctgggctg gggcaattga cactgttggc gacaaagtgc
    76021 tggcaaaagt gctggcgcaa atgaattacg gcggctgcgt ggctgcctgt ggtctggcgg
    76081 gtggttttac tctgccaacc acggtcatgc catttattct gcgtaatgtc cgtttgcaag
    76141 gggtggattc agtaatgacg ccaccagaac gccgcgcaca agcctggcag cgactggtcg
    76201 ccgatttacc ggaatcattc tatacccagg cggcaaaaga gatatctctg tcagaggcac
    76261 cgaactttgc cgaggccatc attaataacc agatccaggg tcgcacgctg gtgaaggtta
    76321 actaaccatt tagcaaggaa taataagaga gggaactcat tttgacatca ttattcaatc
    76381 ggttaacggg aaaagcggtt agccggacag ctttcgtcga acaccttggt caggaagttg
    76441 tacaacatca tccaaactgg aaagtcatga tttcgactga ccacaaattg atgcgcattg
    76501 atacgggtca ccaaacggtg gaaatcaccc ttgatgatgc ctacaaacgt tatcaagccg
    76561 caccagaccc gctgcaagaa gtggctgcag atatgctgaa acagcttgca gaaatgctgc
    76621 cgaccgggcg ctgtattgtt ccggtgatta aagaaaaagc ctggctggat accgttcgcc
    76681 aacagcttag ccagtatgag tctgatccag aggtcctcaa cgcctcgctg gattttgcct
    76741 gggaagactt taacgatgag ttaattatct tgtttgctga agatggtagc gaaaccatgc
    76801 gctatctgat gcgctacgaa gtgaccattg accgcgaaca agccatcagt aatttgaagc
    76861 aaatattgcc ggaagttacg gctgaaatcg ttgatacagg cgcaaacgat ggaaaagttg
    76921 ccgttatctc cgcagggggt acttatgagc ccagtctgat gctcattgac gatctttggg
    76981 tgcaatcacc cgatcctgaa tgcggcgacc tgttagtgac cgttccctcc agaggaaaac
    77041 tgtttttcac ccacaatgat cagccagcaa atctcagtgc attacgacag ctaacaaaac
    77101 aatgcttccg tgaggatgac catcctctga caacacagat attcatccgc cgtaacggtc
    77161 acttttatcc ctgggtagaa accttgcatt aacgcaacct ccgtcatgct tgaaaaagct
    77221 tgtcggagta tgtgatgaaa actgcaaact caccgaagcc gattttgcta ccaaatcggc
    77281 ttttttattg tcaggtggca aattctgaaa gcatcagata gccgcactgc cgcctgacaa
    77341 aacagtttac cttctgcaaa atggggggaa taatctgatt ttgtttgact acaaattaat
    77401 cactcgaacc tatttaatgc tgagcattgt caatcggtta attttgcgtg ctttagcatt
    77461 aacatctatc cagacaatgc agtgaaaatt gggtaatccc cagcaaccgc tgcgtaatgt
    77521 cgtctatctt gtcgcgatcc tggcatccct acattatttg tggtctgtga agattatctc
    77581 attgcagccc ctcatcttcg cagggctggc tttacagctt ttcaccttac gttataagaa
    77641 gttccgtcga tgatggcgct aatttcgtga attgtgcggc ttgttgcaaa ttacacggtg
    77701 ttgaaggtta tttacatgtt agctgttgat tatcttccct gataagacca gtatttagct
    77761 gccaattgct acgaaatcgt tataatgtgc gacctcgtcc tccctgacgc agtttttgcg
    77821 ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt gttttttgcc ggaggatagc
    77881 agcagatcgc tgcacaatgt ccgtcaagtc taacattgac actctggggc aaaatagacc
    77941 ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc tgtcggggca tacgctttac
    78001 agacggcggt gaaacgcctg tcacaatcac actaaacaaa gagtacggaa cccactcatg
    78061 gatattcgta agattaaaaa actgatcgag ctggttgaag aatcaggcat ctccgaactg
    78121 gaaatttctg aaggcgaaga gtcagtacgc attagccgtg cagctcctgc cgcaagtttc
    78181 cctgtgatgc aacaagctta cgctgcacca ataatgcagc agccagctca atctaacgca
    78241 gccgctccgg cgaccgttcc ttccatggaa gcgccagcag cagcggaaat cagtggtcac
    78301 atcgtacgtt ccccgatggt tggtactttc taccgcaccc caagcccgga cgcaaaagcg
    78361 ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc tgtgcatcgt tgaagccatg
    78421 aaaatgatga accagatcga agcggacaaa tccggtaccg tgaaagcaat tctggtcgaa
    78481 agtggacaac cggtagaatt tgacgagccg ctggtcgtca tcgagtaacg aggcgaacat
    78541 gctggataaa attgttattg ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg
    78601 taaagaactg ggcatcaaga ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca
    78661 cgtattactg gcagatgaaa cggtctgtat tggccctgct ccgtcagtaa aaagttatct
    78721 gaacatcccg gcaatcatca gcgccgctga aatcaccggc gcagtagcaa tccatccggg
    78781 ttacggcttc ctctccgaga acgccaactt tgccgagcag gttgaacgct ccggctttat
    78841 cttcatcggc ccgaaagcag aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc
    78901 ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct gacggcccgt tgggcgacga
    78961 tatggataaa aaccgtgcca ttgctaaacg cattggttat ccggtgatta tcaaagcctc
    79021 cggcggcggc ggtggtcgcg gtatgcgcgt agtgcgcggc gacgctgaac tggcacaatc
    79081 catctccatg acccgtgcgg aagcgaaagc tgctttcagc aacgatatgg tttacatgga
    79141 gaaatacctg gaaaatcctc gccacgtcga gattcaggta ctggctgacg gtcagggcaa
    79201 cgctatctat ctggcggaac gtgactgctc catgcagcgc cgccaccaga aagtggtcga
    79261 agaagcacca gcaccgggca ttaccccgga actgcgtcgc tacatcggcg aacgttgcgc
    79321 taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact ttcgagttcc tgttcgaaaa
    79381 cggcgagttc tatttcatcg aaatgaacac ccgtattcag gtagaacacc cggttacaga
    79441 aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt atcgctgccg gtcaaccgct
    79501 gtcgatcaag caagaagaag ttcacgttcg cggccatgcg gtggaatgtc gtatcaacgc
    79561 cgaagatccg aacaccttcc tgccaagccc aggcaaaatc acccgtttcc acgcacctgg
    79621 cggttttggc gtacgttggg agtctcatat ctacgcgggc tacaccgtac cgccgtacta
    79681 tgactcaatg atcggtaagc tgatttgcta cggtgaaaac cgtgacgtgg cgattgcccg
    79741 catgaagaat gcgctgcagg agctgatcat cgacggtatc aaaaccaacg ttgatctgca
    79801 gatccgcatc atgaatgacg agaacttcca gcatggtggc actaacatcc actatctgga
    79861 gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc ggtgttttca tcgcgcttag
    79921 caaagaacgt aatcaacgcc gggcctgtta acgctgcccg gcgtttttat tgccgttagc
    79981 taacggtagc ctgcaatttc cgctttcaac gcgctcattt cagcatcgcc atcaacaatt
    80041 cccagctcaa agcagtattt ctttgtttcc cctggctcaa taaagggcag acgcccctgc
    80101 gctcgttctt tatcacgacc gtcaggaaaa ctattactga cctccagtcc cataacatac
    80161 tcacccgcgc ctaaattttt ccactggata aagttgggta gctgcatggt gtcgaatttc
    80221 tcgtaaattc cccaccccag ttcggcgttc accagagcaa ctttactcat cccctgactg
    80281 tcgctattca acgtgagata gtaaacctgc tcgtcaaaac cgggctgtgg ggtacaaatc
    80341 tccagatgac tggttaaccc ttccgccgca tgcggagttc gtggcgttac gcttttagcc
    80401 gacagcaaga tttctgcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    80461 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    80521 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    80581 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    80641 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    80701 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    80761 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    80821 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    80881 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    80941 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    81001 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    81061 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    81121 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atgggcgtca aaccctagct
    81181 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    81241 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    81301 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    81361 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    81421 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    81481 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    81541 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    81601 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    81661 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    81721 tagcttccag aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    81781 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    81841 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    81901 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    81961 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    82021 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    82081 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    82141 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    82201 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttgc ccggaataga
    82261 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    82321 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    82381 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    82441 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    82501 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    82561 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    82621 agccaccatt tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa
    82681 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    82741 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    82801 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    82861 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    82921 gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc cgaaggtttc ggactggcaa
    82981 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    83041 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    83101 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    83161 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    83221 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    83281 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    83341 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    83401 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    83461 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    83521 taggattagc gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    83581 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    83641 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    83701 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    83761 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    83821 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    83881 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    83941 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    84001 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    84061 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc agttccgcga
    84121 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    84181 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    84241 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    84301 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    84361 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    84421 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    84481 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    84541 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    84601 ctaccggttt accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    84661 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    84721 ctttcatgcc gtcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    84781 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    84841 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacca ggctgttcag
    84901 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    84961 ccaccgattg tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt
    85021 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    85081 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    85141 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca
    85201 cttttttagt aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgattggcaa
    85261 tagagacact taaattgaca ttgtcttttt ttgcctcttc tttcatggca ttcgcaagat
    85321 caatatagaa aggatgttgc tgtgttaata atgacgcacc aattttatac tcttttgcca
    85381 acgaattttg cgagaacaat gccaagctca ccaacatagc tgaaccaagt aatttaatat
    85441 ttttcataaa caatcctcat tgtttttgta gggtacaacg tcttaaattt ttccttcact
    85501 accacagaca ccaataatac gagttacgac ttctttcatg gcggcctttg cgggctgcat
    85561 atagtggcgt ggatcatttg ccgcaggatt gttgtagaaa tatcctttta acgcatcggc
    85621 aaacgcaatt ttcaattctg tcgcgacatt cactttacaa ataccgcggc gaatacagtg
    85681 ctgaacgtcc tgttctgata agcccgaagc accatgcaac accagtggaa tctccactcg
    85741 ctgacgaatc tgttccagcc gcacaaaatc cagtcgtggt tcatgcgcat ataaaccatg
    85801 tgctgtgccg attgctacgg ccagggagtc aatttgggta cgttgaacaa actctgcagc
    85861 tgcggctggt gaggtataag cggaatcttt ttcatccacc agcagatcgt cctcctggcc
    85921 gccaagacgc cctagctcgg cctcaacgct aacatcaaag cgatggcaaa aggtggtaac
    85981 ttcttgcact cgcgcaatgt tgtcttcaaa tggcaaatgg gagccatcaa tcatgacaga
    86041 acggatccct gcgcgaacct tgttaaaaat gtcatcactc tcttcatgat ggtcgagatg
    86101 caggactaac ggcaggttgt actcgcgagc cagcgattgt actaacgaaa ttaaacttcc
    86161 cacgccacca taacgataag taccgggtgt tcccgccagc ataacgggag agcgaagttg
    86221 tgcagcgctc tcaacaacta cctgtaaagt ttcaaggtta tgaatgttga aagcggggac
    86281 ggcatatccc tctgactggg cttttttcag catgtttttg cttgaaatga tgtacatcgc
    86341 agctcctgtt atttgtcgtt cgccttacaa aatgaagaaa gagaatattc actttcattt
    86401 tgaaagcaaa ctagcacagc aaaagcggca aaaagcgtga tgcatgtcga atatcatcaa
    86461 aataacttac aaaaaaataa cttaatttgt ttttcact
//
LOCUS       DAZUFB010000008        81884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZUFB010000008 DAZUFB010000000
VERSION     DAZUFB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.008"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..325
                     /gene="iraP"
                     /locus_tag="ACHY3M_000645"
     CDS             65..325
                     /gene="iraP"
                     /locus_tag="ACHY3M_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFS4315619.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            426..1841
                     /gene="phoA"
                     /locus_tag="ACHY3M_000646"
     CDS             426..1841
                     /gene="phoA"
                     /locus_tag="ACHY3M_000646"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFS4315620.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            1960..2280
                     /gene="psiF"
                     /locus_tag="ACHY3M_000647"
     CDS             1960..2280
                     /gene="psiF"
                     /locus_tag="ACHY3M_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFS4315621.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSYCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2382..3497
                     /gene="adrA"
                     /locus_tag="ACHY3M_000648"
                     /gene_synonym="dgcC"
     CDS             2382..3497
                     /gene="adrA"
                     /locus_tag="ACHY3M_000648"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFS4315622.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3514..4323)
                     /gene="proC"
                     /locus_tag="ACHY3M_000649"
     CDS             complement(3514..4323)
                     /gene="proC"
                     /locus_tag="ACHY3M_000649"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4315623.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4443..4901
                     /gene="yaiI"
                     /locus_tag="ACHY3M_000650"
     CDS             4443..4901
                     /gene="yaiI"
                     /locus_tag="ACHY3M_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4315624.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5084..5608
                     /gene="aroL"
                     /locus_tag="ACHY3M_000651"
     CDS             5084..5608
                     /gene="aroL"
                     /locus_tag="ACHY3M_000651"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFS4315625.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5658..5849
                     /gene="yaiA"
                     /locus_tag="ACHY3M_000652"
     CDS             5658..5849
                     /gene="yaiA"
                     /locus_tag="ACHY3M_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFS4315626.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6107..6784
                     /gene="aroM"
                     /locus_tag="ACHY3M_000653"
     CDS             6107..6784
                     /gene="aroM"
                     /locus_tag="ACHY3M_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFS4315627.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            6856..7140
                     /gene="ppnP"
                     /locus_tag="ACHY3M_000654"
     CDS             6856..7140
                     /gene="ppnP"
                     /locus_tag="ACHY3M_000654"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFS4315628.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7288..7629
                     /locus_tag="ACHY3M_000655"
                     /pseudo
     CDS             7288..7629
                     /locus_tag="ACHY3M_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7626..7709
                     /locus_tag="ACHY3M_000656"
     CDS             7626..7709
                     /locus_tag="ACHY3M_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315629.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(7787..8698)
                     /gene="rdgC"
                     /locus_tag="ACHY3M_000657"
     CDS             complement(7787..8698)
                     /gene="rdgC"
                     /locus_tag="ACHY3M_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFS4315630.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMESPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8823..9731
                     /gene="mak"
                     /locus_tag="ACHY3M_000658"
     CDS             8823..9731
                     /gene="mak"
                     /locus_tag="ACHY3M_000658"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFS4315631.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9875..11059)
                     /gene="araJ"
                     /locus_tag="ACHY3M_000659"
     CDS             complement(9875..11059)
                     /gene="araJ"
                     /locus_tag="ACHY3M_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFS4315632.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAELFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11185..14328)
                     /gene="sbcC"
                     /locus_tag="ACHY3M_000660"
     CDS             complement(11185..14328)
                     /gene="sbcC"
                     /locus_tag="ACHY3M_000660"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFS4315633.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDKQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(14325..15527)
                     /gene="sbcD"
                     /locus_tag="ACHY3M_000661"
     CDS             complement(14325..15527)
                     /gene="sbcD"
                     /locus_tag="ACHY3M_000661"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFS4315634.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            15717..16406
                     /gene="phoB"
                     /locus_tag="ACHY3M_000662"
     CDS             15717..16406
                     /gene="phoB"
                     /locus_tag="ACHY3M_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFS4315635.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16464..17759
                     /gene="phoR"
                     /locus_tag="ACHY3M_000663"
     CDS             16464..17759
                     /gene="phoR"
                     /locus_tag="ACHY3M_000663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFS4315636.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFIQGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            18166..19485
                     /gene="brnQ"
                     /locus_tag="ACHY3M_000664"
     CDS             18166..19485
                     /gene="brnQ"
                     /locus_tag="ACHY3M_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFS4315637.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19561..20934
                     /gene="proY"
                     /locus_tag="ACHY3M_000665"
     CDS             19561..20934
                     /gene="proY"
                     /locus_tag="ACHY3M_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFS4315638.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            21090..22907
                     /gene="malZ"
                     /locus_tag="ACHY3M_000666"
     CDS             21090..22907
                     /gene="malZ"
                     /locus_tag="ACHY3M_000666"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFS4315639.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHRQLTDGILALPAISATVWMN"
     gene            complement(22912..23493)
                     /gene="acpH"
                     /locus_tag="ACHY3M_000667"
     CDS             complement(22912..23493)
                     /gene="acpH"
                     /locus_tag="ACHY3M_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFS4315640.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            23586..24656
                     /gene="queA"
                     /locus_tag="ACHY3M_000668"
     CDS             23586..24656
                     /gene="queA"
                     /locus_tag="ACHY3M_000668"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4315641.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIIHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24712..25839
                     /gene="tgt"
                     /locus_tag="ACHY3M_000669"
     CDS             24712..25839
                     /gene="tgt"
                     /locus_tag="ACHY3M_000669"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4315642.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25862..26194
                     /gene="yajC"
                     /locus_tag="ACHY3M_000670"
     CDS             25862..26194
                     /gene="yajC"
                     /locus_tag="ACHY3M_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4315643.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26222..28069
                     /gene="secD"
                     /locus_tag="ACHY3M_000671"
     CDS             26222..28069
                     /gene="secD"
                     /locus_tag="ACHY3M_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFS4315644.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28080..29051
                     /gene="secF"
                     /locus_tag="ACHY3M_000672"
     CDS             28080..29051
                     /gene="secF"
                     /locus_tag="ACHY3M_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFS4315645.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29180..29527
                     /gene="yajD"
                     /locus_tag="ACHY3M_000673"
     CDS             29180..29527
                     /gene="yajD"
                     /locus_tag="ACHY3M_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFS4315646.1"
                     /translation="MAIIPKNYARLESGYREKALKIYLWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29704..30588)
                     /gene="tsx"
                     /locus_tag="ACHY3M_000674"
     CDS             complement(29704..30588)
                     /gene="tsx"
                     /locus_tag="ACHY3M_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFS4315647.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30887..31426)
                     /gene="yajI"
                     /locus_tag="ACHY3M_000675"
     CDS             complement(30887..31426)
                     /gene="yajI"
                     /locus_tag="ACHY3M_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS4315648.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            31577..32026
                     /gene="nrdR"
                     /locus_tag="ACHY3M_000676"
     CDS             31577..32026
                     /gene="nrdR"
                     /locus_tag="ACHY3M_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4315649.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            32030..33133
                     /gene="ribD"
                     /locus_tag="ACHY3M_000677"
     CDS             32030..33133
                     /gene="ribD"
                     /locus_tag="ACHY3M_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFS4315650.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33222..33692
                     /gene="ribE"
                     /locus_tag="ACHY3M_000678"
     CDS             33222..33692
                     /gene="ribE"
                     /locus_tag="ACHY3M_000678"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFS4315651.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33712..34131
                     /gene="nusB"
                     /locus_tag="ACHY3M_000679"
     CDS             33712..34131
                     /gene="nusB"
                     /locus_tag="ACHY3M_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4315652.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34209..35186
                     /gene="thiL"
                     /locus_tag="ACHY3M_000680"
     CDS             34209..35186
                     /gene="thiL"
                     /locus_tag="ACHY3M_000680"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFS4315653.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            35164..35682
                     /gene="pgpA"
                     /locus_tag="ACHY3M_000681"
     CDS             35164..35682
                     /gene="pgpA"
                     /locus_tag="ACHY3M_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4315654.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSSIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(35736..36710)
                     /gene="yajO"
                     /locus_tag="ACHY3M_000682"
     CDS             complement(35736..36710)
                     /gene="yajO"
                     /locus_tag="ACHY3M_000682"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFS4315655.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(36890..38752)
                     /gene="dxs"
                     /locus_tag="ACHY3M_000683"
     CDS             complement(36890..38752)
                     /gene="dxs"
                     /locus_tag="ACHY3M_000683"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4315656.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(38777..39676)
                     /gene="ispA"
                     /locus_tag="ACHY3M_000684"
     CDS             complement(38777..39676)
                     /gene="ispA"
                     /locus_tag="ACHY3M_000684"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFS4315657.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(39676..39918)
                     /gene="xseB"
                     /locus_tag="ACHY3M_000685"
     CDS             complement(39676..39918)
                     /gene="xseB"
                     /locus_tag="ACHY3M_000685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4315658.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            40124..41572
                     /gene="thiI"
                     /locus_tag="ACHY3M_000686"
     CDS             40124..41572
                     /gene="thiI"
                     /locus_tag="ACHY3M_000686"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4315659.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41626..42216)
                     /gene="yajL"
                     /locus_tag="ACHY3M_000687"
     CDS             complement(41626..42216)
                     /gene="yajL"
                     /locus_tag="ACHY3M_000687"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFS4315660.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(42179..43090)
                     /gene="panE"
                     /locus_tag="ACHY3M_000688"
     CDS             complement(42179..43090)
                     /gene="panE"
                     /locus_tag="ACHY3M_000688"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4315661.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43258..43749
                     /gene="yajQ"
                     /locus_tag="ACHY3M_000689"
     CDS             43258..43749
                     /gene="yajQ"
                     /locus_tag="ACHY3M_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFS4315662.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43877..45241)
                     /gene="yajR"
                     /locus_tag="ACHY3M_000690"
     CDS             complement(43877..45241)
                     /gene="yajR"
                     /locus_tag="ACHY3M_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4315663.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSELTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            45649..46584
                     /locus_tag="ACHY3M_000691"
     CDS             45649..46584
                     /locus_tag="ACHY3M_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4315664.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(46640..47182)
                     /locus_tag="ACHY3M_000692"
     CDS             complement(46640..47182)
                     /locus_tag="ACHY3M_000692"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4315665.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(47166..47477)
                     /locus_tag="ACHY3M_000693"
     CDS             complement(47166..47477)
                     /locus_tag="ACHY3M_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFS4315666.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(47662..48552)
                     /gene="cyoE"
                     /locus_tag="ACHY3M_000694"
     CDS             complement(47662..48552)
                     /gene="cyoE"
                     /locus_tag="ACHY3M_000694"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFS4315667.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(48564..48893)
                     /gene="cyoD"
                     /locus_tag="ACHY3M_000695"
     CDS             complement(48564..48893)
                     /gene="cyoD"
                     /locus_tag="ACHY3M_000695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFS4315668.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(48893..49507)
                     /gene="cyoC"
                     /locus_tag="ACHY3M_000696"
     CDS             complement(48893..49507)
                     /gene="cyoC"
                     /locus_tag="ACHY3M_000696"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFS4315669.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(49497..51488)
                     /gene="cyoB"
                     /locus_tag="ACHY3M_000697"
     CDS             complement(49497..51488)
                     /gene="cyoB"
                     /locus_tag="ACHY3M_000697"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFS4315670.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(51510..52457)
                     /gene="cyoA"
                     /locus_tag="ACHY3M_000698"
     CDS             complement(51510..52457)
                     /gene="cyoA"
                     /locus_tag="ACHY3M_000698"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFS4315671.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(52917..54392)
                     /gene="ampG"
                     /locus_tag="ACHY3M_000699"
     CDS             complement(52917..54392)
                     /gene="ampG"
                     /locus_tag="ACHY3M_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFS4315672.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(54436..55014)
                     /gene="yajG"
                     /locus_tag="ACHY3M_000700"
     CDS             complement(54436..55014)
                     /gene="yajG"
                     /locus_tag="ACHY3M_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4315673.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            55319..55636
                     /gene="bolA"
                     /locus_tag="ACHY3M_000701"
     CDS             55319..55636
                     /gene="bolA"
                     /locus_tag="ACHY3M_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFS4315674.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            55980..57278
                     /gene="tig"
                     /locus_tag="ACHY3M_000702"
     CDS             55980..57278
                     /gene="tig"
                     /locus_tag="ACHY3M_000702"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4315675.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            57524..58147
                     /gene="clpP"
                     /locus_tag="ACHY3M_000703"
     CDS             57524..58147
                     /gene="clpP"
                     /locus_tag="ACHY3M_000703"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4315676.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            58273..59547
                     /gene="clpX"
                     /locus_tag="ACHY3M_000704"
     CDS             58273..59547
                     /gene="clpX"
                     /locus_tag="ACHY3M_000704"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFS4315677.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            59735..62089
                     /gene="lon"
                     /locus_tag="ACHY3M_000705"
     CDS             59735..62089
                     /gene="lon"
                     /locus_tag="ACHY3M_000705"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFS4315678.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            62280..62570
                     /gene="hupB"
                     /locus_tag="ACHY3M_000706"
     CDS             62280..62570
                     /gene="hupB"
                     /locus_tag="ACHY3M_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFS4315679.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            62762..64633
                     /gene="ppiD"
                     /locus_tag="ACHY3M_000707"
     CDS             62762..64633
                     /gene="ppiD"
                     /locus_tag="ACHY3M_000707"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4315680.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSLYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFTLPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            64784..65155
                     /gene="ybaV"
                     /locus_tag="ACHY3M_000708"
     CDS             64784..65155
                     /gene="ybaV"
                     /locus_tag="ACHY3M_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4315681.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            65249..65647
                     /gene="fadM"
                     /locus_tag="ACHY3M_000709"
     CDS             65249..65647
                     /gene="fadM"
                     /locus_tag="ACHY3M_000709"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFS4315682.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(65699..66394)
                     /gene="queC"
                     /locus_tag="ACHY3M_000710"
     CDS             complement(65699..66394)
                     /gene="queC"
                     /locus_tag="ACHY3M_000710"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFS4315683.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(66459..68159)
                     /gene="ybaE"
                     /locus_tag="ACHY3M_000711"
     CDS             complement(66459..68159)
                     /gene="ybaE"
                     /locus_tag="ACHY3M_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFS4315684.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            68259..69077
                     /gene="cof"
                     /locus_tag="ACHY3M_000712"
     CDS             68259..69077
                     /gene="cof"
                     /locus_tag="ACHY3M_000712"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFS4315685.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            69230..69688
                     /gene="decR"
                     /locus_tag="ACHY3M_000713"
     CDS             69230..69688
                     /gene="decR"
                     /locus_tag="ACHY3M_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFS4315686.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            69718..71490
                     /gene="mdlA"
                     /locus_tag="ACHY3M_000714"
     CDS             69718..71490
                     /gene="mdlA"
                     /locus_tag="ACHY3M_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS4315687.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVASNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            71483..73264
                     /gene="mdlB"
                     /locus_tag="ACHY3M_000715"
     CDS             71483..73264
                     /gene="mdlB"
                     /locus_tag="ACHY3M_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS4315688.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            73445..73783
                     /gene="glnK"
                     /locus_tag="ACHY3M_000716"
     CDS             73445..73783
                     /gene="glnK"
                     /locus_tag="ACHY3M_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4315689.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            73813..75099
                     /gene="amtB"
                     /locus_tag="ACHY3M_000717"
     CDS             73813..75099
                     /gene="amtB"
                     /locus_tag="ACHY3M_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFS4315690.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWGVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(75147..76007)
                     /gene="tesB"
                     /locus_tag="ACHY3M_000718"
     CDS             complement(75147..76007)
                     /gene="tesB"
                     /locus_tag="ACHY3M_000718"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFS4315691.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            76225..76797
                     /gene="ybaY"
                     /locus_tag="ACHY3M_000719"
     CDS             76225..76797
                     /gene="ybaY"
                     /locus_tag="ACHY3M_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFS4315692.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(76828..77139)
                     /locus_tag="ACHY3M_000720"
     CDS             complement(76828..77139)
                     /locus_tag="ACHY3M_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFS4315693.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            77302..77398
                     /gene="ffs"
                     /locus_tag="ACHY3M_000721"
     ncRNA           77302..77398
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHY3M_000721"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            77518..77871
                     /gene="ybaA"
                     /locus_tag="ACHY3M_000722"
     CDS             77518..77871
                     /gene="ybaA"
                     /locus_tag="ACHY3M_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFS4315694.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(77913..79463)
                     /gene="pdeB"
                     /locus_tag="ACHY3M_000723"
     CDS             complement(77913..79463)
                     /gene="pdeB"
                     /locus_tag="ACHY3M_000723"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFS4315695.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRLVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(79627..80097)
                     /gene="ylaC"
                     /locus_tag="ACHY3M_000724"
     CDS             complement(79627..80097)
                     /gene="ylaC"
                     /locus_tag="ACHY3M_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFS4315696.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFAALAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(80213..80764)
                     /gene="maa"
                     /locus_tag="ACHY3M_000725"
     CDS             complement(80213..80764)
                     /gene="maa"
                     /locus_tag="ACHY3M_000725"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFS4315697.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(80936..81154)
                     /gene="hha"
                     /locus_tag="ACHY3M_000726"
     CDS             complement(80936..81154)
                     /gene="hha"
                     /locus_tag="ACHY3M_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFS4315698.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(81180..81554)
                     /gene="tomB"
                     /locus_tag="ACHY3M_000727"
     CDS             complement(81180..81554)
                     /gene="tomB"
                     /locus_tag="ACHY3M_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFS4315699.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19941 a        20230 c        21836 g        19877 t
ORIGIN      
        1 agacactatg aagttgtgaa acataatgtt aacttctcca tactttggat aaggaaatac
       61 agacatgaaa aatctcattg ctgagttgtt atttaagctt gcccaaaaag aagaagagtc
      121 gaaagaactg tgtgcgcagg tagaagcttt ggagattatc gtcactgcaa tgcttcgcaa
      181 tatggcgcaa aatgaccaac agcgcttgat tgatcaggta gagggggcgc tgtacgaggt
      241 aaagcccgat gccagcattc ctgacgacga tacggagctg ctgcgcgatt acgtaaagaa
      301 gttattgaag catcctcgtc agtaaaaagt taatcttttc aacagctgtc ataaagttgt
      361 cacggccgag acttatagtc gctttgtttt tattttttaa tgtatttgta catggagaaa
      421 ataaagtgaa acaaagcact attgcactgg cactcttatc gttactgttt acccctgtga
      481 caaaagcccg gacaccagaa atgcctgttc tggaaaaccg ggctgctcag ggcgatatta
      541 ctgcatccgg cggtgctcgc cgtttaacgg gtgatcagac tgccgctctg cgtgattctc
      601 ttagcgataa acctgcaaaa aatattattt tgctgattgg cgatgggatg ggggattcgg
      661 aaattacagc cgcacgtaat tatgccgaag gtgcgggcgg cttttttaaa ggtatcgatg
      721 ccttaccgct taccgggcaa tacactcact atgcgctgaa taaaaaaacc ggcaaaccgg
      781 actacgtcac cgattcggct gcatcagcaa ccgcctggtc aaccggtgtc aaaacctata
      841 acggcgcgct gggcgtcgat attcacgaaa aagatcaccc aacgattctg gaaatggcaa
      901 aagccgcagg tctggcgacc ggtaacgttt ctaccgcaga gttgcaggat gccacgcccg
      961 ctgcgctggt ggcgcatgtg acctcgcgca aatgctacgg tccgagcgcg accagtgaaa
     1021 aatgtccggg taacgctctg gaaaaaggcg gaaaaggatc gattaccgaa cagctgctta
     1081 acgctcgtgc cgacgttacg cttggcggtg gcgcaaaaac ctttgctgaa acggcaaccg
     1141 ccggtgaatg gcagggaaaa acgctgcgtg aacaggcaca ggcgcgtggt tatcagttgg
     1201 tcagcgatgc tgcctcactg aattcggtga cggaagcgaa tcagcaaaaa cctctgcttg
     1261 gactgtttgc tgacggcaat atgccagtgc gctggctagg accgaaagca acgtaccacg
     1321 gcaatatcga taagcccgca gtcacctgta cgcctaatcc gcaacgtaat gacagtgtac
     1381 caaccctggc gcagatgacc gacaaagcca ttgaattgtt gagtaaaaat gagaaaggat
     1441 ttttcctgca agttgaaggt gcgtcaatcg ataaacagga tcacgctgcg aatccttgtg
     1501 ggcaaattgg cgagacggtc gatctcgatg aagccgtaca acgggcgctg gaattcgcta
     1561 aaaaggatgg caacacgctg gtcatagtca ccgctgatca cgcccacgcc agccagattg
     1621 tcgcgccgga taccaaagct ccgggcctca cccaggcgct aaataccaaa gatggcgcag
     1681 tgatggtgat gagctacggg aactccgaag aggattcaca agaacatacc ggcagtcagt
     1741 tgcgtattgc ggcgtatggc ccgcatgccg ccaatgttgt tggactgacc gaccagaccg
     1801 atctcttcta caccatgaaa gccgccctgg ggctgaaata aaaccgcgcc cggcagtgaa
     1861 ttttcgctgc cgggtggttt ttttgctgtt agcaaccaga cttaatggca gatcacgggc
     1921 gcatacgctc atggttaaaa catgaagagg gatggtgcta tgaaaataac attactggtt
     1981 accttgcttt ttggtctggt ttttttaacc accgtcggcg ctgccgagag aaccttaacc
     2041 ccacaacaac agcgtatgac atcctgtaat cagcaggcga cggcgcaggc gttgaaaggg
     2101 gatgctcgta agacctacat gagttattgc ctgaagaaca gcaagtctgc gcctggtgaa
     2161 aaaagtttga cgccacagca gcaaaagatg cgcgaatgta ataatcaagc aacgcaacaa
     2221 tctctgaaag gtgatgatcg taataagttt atgagtgcct gcctcaagaa agccgcctga
     2281 tacctgatag tgctaacggg tgagctacga aagtggctca cccgaaattt catacttctg
     2341 cctttagccc cgtctctata atttgggaaa attgtttctg aatgttccca aaaataatga
     2401 atgatgaaaa ctttttcaaa aaagcggcgg cgcatgggga ggaacctcct ttaactcctc
     2461 aaaacgaaca tcagcggtcc gggctgcgct tcgcccgtcg cgtcagacta ccccgtgcgg
     2521 ttggcctggc tggcatgttc ttaccgattg cttcaacgct ggtttcgcac ccgccgccgg
     2581 gctggtggtg gctggtgttg gtcggctggg cgttcgtctg gccgcattta gcctggcaga
     2641 tagcgagcag ggccgtcgat ccgcttagcc gggaaattta caacttaaaa accgatgcag
     2701 tattagcggg aatgtgggta ggcgtaatgg gcgtaaacgt gctgccttcc accgctatgt
     2761 tgatgattat gtgtctgaat ttgatggggg caggcggccc ccgtctgttt gtcgcgggtc
     2821 tggtgttgat ggtggtttcc tgccttgtca ccctcgagct tacgggcatt accgtgtcgt
     2881 tcaatagtgc gccgctggaa tggtggctct cccttcccat tattgtcgtt tatcctctgc
     2941 tgtttggctg ggtcagctac cagacggcaa ccaaactggc ggaacataaa cgcaggttgc
     3001 aggtcatgag tacccgcgac ggcatgacgg gcgtgtataa ccgacgtcat tgggaaacta
     3061 tgttacgcaa tgaatttgat aactgtcggc ggcataatcg cgatgcaacg ttactgatta
     3121 tcgatatcga ccatttcaag agcatcaacg atacctgggg tcatgatgtg ggcgatgaag
     3181 cgattgtggc gcttacccga cagttacaaa ttaccctgcg cggtagcgat gtgattggtc
     3241 ggtttggcgg cgatgagttt gcagtaatca tgtccggtac gccagctgag agcgccatta
     3301 ccgccatgtt acgggtgcgt gaagggctaa acacgttacg cttgccgaat acgccacagg
     3361 taactttacg gattagtgtg ggggttgcgc cgctgaaccc acaaatgagt cactatcgtg
     3421 agtggttgaa atcggcagat ttggcgcttt acaaagcaaa gaaagccgga cgtaaccgca
     3481 ccgaagtggc ggcctgacgt ccggcgaaag tcatcaggat ttgctgagtt tttctgattt
     3541 ttccatacac ttcgtcatcg cttcgatcac tgcggcacgg aagcctttct cttccagtac
     3601 gcgtaccgct tcaatggtgg tgcctcccgg tgagcagacc atatctttca gtgcccccgg
     3661 atgttctccc gtttccagca ccatttttgc ggaacccatt accgcctgag cggcaaattt
     3721 atacgcctgg gcgcgtggca tcccgcccag cacggcggcg tcggccatcg cttcgataaa
     3781 cataaatacg taggctggcg aagaaccgct cacaccgacc accgggtgga tcatcggctc
     3841 agcaattact tccgcttcgc caaagcagcg gaaaatattc agcacatcag cggtatcttc
     3901 tggggttacc agcgcgtttg gcgttacgga ggtcatcccg gcattaacca gcgcgggagt
     3961 gttcggcatg gcgcggataa ttttccggtc atggcccagc gcgcgggcaa gctggtcgag
     4021 cgtgacacct gcagcaatag aaacgaccag agagtcttta ttcaggctgg aggtgatttc
     4081 gctaagcact ttaatcatga tgccaggttt aacggcagca aaaatgatgt cggcgatttg
     4141 cgccacttct tgcgccgatt ctgcggcgtt gatgccgaac tggtcatgca gggcggcgac
     4201 tttatccggg gagggggtgt atacccagat ttgccctgga agcacctgac cgctggcaat
     4261 cagaccgccg agaatggctt ttcccatatt gccgcagcca ataaaaccga ttttcttttc
     4321 cattgcctca ctcctgccgt gaaattcatt gttttgataa tcgctggcag aagcataaac
     4381 agaactatgc cggaaggcaa aagcgcgaca caatagagga ttacccaaca aaggatgact
     4441 ttatgacaat ttgggtggat gccgacgcgt gtcccaatgt aattaaagag attttgtatc
     4501 gcgcggcgga acgtatgcag atgccgctgg tactggtagc aaaccagagt ttacgcgtgc
     4561 cgccatcgcg atttattcgt acgctgcgcg tcgcggcagg tttcgacgtt gccgataacg
     4621 aaattgtccg gcagtgtgaa gcgggcgatt tggtgatcac cgcagatata cctttggctg
     4681 ctgaagccat cgagaaaggc gcagcggcgc ttaatccgcg cggcgaacgt tacacgccag
     4741 cgaccattcg tgagcgcctg acgatgcgcg attttatgga taccttacgt gccagtggga
     4801 tccagaccgg cggaccagat agcctttcac aacgtgaccg ccaggccttt gccgcggagc
     4861 tggagaagtg gtggctggaa gtgcaacgta gtcgtggcta aatgtaattt attatttaca
     4921 cttcattctt gaatatttat tggtatagta agggatgtat tgagattttt actttaagtg
     4981 gaattttttc tttacaatcg aaattgtact agtttgatgg tatgatcgct attctcatga
     5041 caccggcttt cgccgcattg cgacctattg gggaaaaccc acgatgacac aacctctttt
     5101 tctgatcggg cctcggggct gtggtaaaac aacggtcgga atggcccttg ccgattcgct
     5161 taaccgtcgg tttgtcgata ccgatcagtg gttgcaatca cagctcaata tgacggtcgc
     5221 ggagatcgtc gaaagggaag agtgggcggg atttcgcgcc agagaaacgg cggcgctgga
     5281 agcggtaact gcgccatcca ccgttatcgc tacaggcggc ggcattattc tgacggaatt
     5341 taatcgtcac ttcatgcaaa ataacgggat cgtggtttat ttgtgtgcgc cagtatcagt
     5401 cctggttaac cgactgcaag ctgcaccgga agaagattta cggccaacct taacgggaaa
     5461 accgctgagc gaagaagttc aggaagtgct ggaagaacgc gatgcgctat atcgcgaagt
     5521 tgcgcatatt atcatcgacg caacaaacga acccagccag gtgatttctg aaattcgtag
     5581 cgccctggca cagacgatca attgttgatt ttagagcgcc tatacttaac gttcatcccg
     5641 tgaaataagg aagaacgatg ccaacgaaac cgccttatcc tcgtgaagca tatatagtga
     5701 cgattgaaaa aggaaagcca ggacagacgg taacctggta ccaactcaga gccgatcatc
     5761 ctaaaccaga ctcgttgatc agtgaacatc cgacggctca ggaagcgatg gatgcgaaaa
     5821 aacgctatga ggaccctgac aaagagtgac cgcatcagac tgctcggaag ggattctgag
     5881 tgccactaca agggatctgc gtcacatttt tcataattca tgtttttcta ataattagaa
     5941 tattaaacaa taacaatcca ttactggaat catttggaat ctttacatta tgccgtgcac
     6001 gtctgctgct acgctttttg tcatttgtag cacaagtaag tgtcagcagt ggtgcttcac
     6061 acttgcccgg taattaacga cgaaagaaaa gtaaggtgga tgaacaatga gtgcgtcgtt
     6121 ggcgatcctg accatcggca ttgtacctat gcaggaggtt ttgccgctcc tgacggaata
     6181 cattgacgaa gataatattt cccatcatag cttgctgggg aagttaagtc gtgaagaagt
     6241 gatggcggag tacgcgccag aagcaggcga agacaccatt ctcacattat taaatgacaa
     6301 ccagctggcc catgtttcgc gtcgtaaagt ggagcgagac ctgcaaggtg tggttgaagt
     6361 gctcgataat cagggttatg acgtcatttt attaatgagt acagcaaaca ttagtagtat
     6421 gactgcgcgt aatacgatct ttcttgagcc gtcgcgaata ttgcctccac tggtttcctc
     6481 tattgttgaa gatcatcagg tgggggttat cgttccggtt gaggagatgc tgaccgttca
     6541 ggcgcaaaaa tggcaaattt tgcagaaatc gccggtattt tcattgggca accccattca
     6601 tgattcagaa caaaaaatca ttgatgccgg gaaagaatta ctggcaaaag gggctgatgt
     6661 catcatgctg gattgtttag gatttcacca acgtcatcgc gatttactgc aaaaacagct
     6721 cgatgttcct gtcttgctgt ctaacgtatt gattgcacgg ctggctgcgg aattactggt
     6781 gtaattttgc gtgacagcca gcgcctctgg cccctatagt gaagtagatg ttcaactacc
     6841 agacagggcc agtttatgct tcaaagtaat gagtactttt ccggcaaagt gaaatcaatc
     6901 ggcttttcca gcagcagcac tggtcgcgcc agcgtgggtg ttatggttga aggcgaatac
     6961 accttcagca ccgctgagcc ggaagagatg acggtaatca gtggcgcgct gaatgtgtta
     7021 ctgcctgacg cgaccgactg gcaggtgtat gaagccggtt cggtgtttaa tgttcccggt
     7081 cacagtgagt ttcatctgca agttgccgaa cccacctctt atctgtgccg ctatctgtaa
     7141 ttcctcgcct tccccttgaa cgggagggca tttttctgaa atatcctctc tttaacccat
     7201 aataatattt cctttgctgc gattttttca atttccggta tattcataat tcatcaaggt
     7261 tgatataaat atcagtgaag atctccagat attgttgcgg aactggctac gataaaagat
     7321 aaatcagatg atgaatggtg gcgtgcattg ctgcaaagtc gcaatgatca tttgcgccaa
     7381 acggcattac gcaacgcaca taccccggcg tcgttgttaa cgacattgac cgagtctcgg
     7441 catcggtcgc tagctatgaa taatccacag ctcgctgccg atgtgaaaac gacgtggtta
     7501 aaagaggatc catcattact cttatttgtc gaacaacccg atctttcgct gttacgggat
     7561 ttagtgaaaa ccggggcaat gcggaaaatt cgcagtgaag cgcgtcactg gcttgaggaa
     7621 aaacaatgac acaacgtcca tggagcaagt tacagcataa aacgcataat attgctgcgc
     7681 taaaaattat tgcccgccgt agcgaataat tatatgcctg gtgtggcttc gtacgccgga
     7741 taagacgcgg caggcgtcgc atccggcatt aaaggaaaat cagcaattaa cgttgtgctt
     7801 cgccacctaa tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga
     7861 tgaaatcggc atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgc
     7921 gcagctcgtc gcagaacttc agacgcttga gcgaaccatc gtcgcacatc acaaactgaa
     7981 tgcgctgctg ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtgattgg
     8041 tgatctcttc gctggtcaga tcttgtttct tcgcgcggat cacgccgcca tcttccagca
     8101 acgatttcag ctcggcttca tcaagcagct ggaagccctg tgccgcacta ccggagcgaa
     8161 cccattcggt cagcgtcagt tcaatcgggc tttccatgct caatggtaca accggtaacg
     8221 accccaggct tttacgcagt aatgccagcg tatcttccgc ttttttggca ctggcgcagt
     8281 ccaccataat caaaccgtta accgtgtcga tccacatcat tgtctggcta aaacggctga
     8341 aagcacgcgg cagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct
     8401 tcagcttacg cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa
     8461 tcaccggaga cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg
     8521 caacgtgcgt taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca
     8581 tgtcctggct gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg
     8641 cacgcagcga aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga
     8701 taatttccac ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt
     8761 gcattgccaa tccggttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta
     8821 ccgtgcgtat aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg
     8881 caggggagca gttgtaccgc catcgtctgc ccacgccgcg tgatgattac cggcaaacta
     8941 ttgaaacgat cgccacgttg gttgatatgg cggagcaggc gacagggcag cgcggaacgg
     9001 taggtatggg cattccaggt tcgatttcgc cctacaccgg tgtggtgaag aatgccaatt
     9061 caacctggct caacggtcag ccattcgata aagacttaag cgcgcggttg cagcgggaag
     9121 tgcggctggc aaatgacgcc aactgtctgg cggtttcaga agcggtagat ggcgcggcag
     9181 cgggagcgca gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat
     9241 tcaatgggcg ggcgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc
     9301 taccgtggat ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta
     9361 aacaaggttg tattgaaacc tttatttcgg gcactggatt cgcgacggat tatcgtcgtt
     9421 tgagcggaca tgcgctgaaa ggcagtgaaa ttatcagcct ggttgaagaa agcgatccgg
     9481 tagcggaact ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg
     9541 tgaatattct cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt
     9601 tatatcaaac ggttccgcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc
     9661 cggtgcgtaa ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc
     9721 cacaagagta aaaaacgtag gcaattggcg catcatgcct gatgcgacgc ttgccgcgtc
     9781 ttatcagtat ctacaaaatg tgccagaacc gcgtagggcg gataaggcgt tcacgccgca
     9841 tccggcaata agcaacgcgc accgtgacag taatctaccc cagtggtttc gccagcaccg
     9901 gactatccgc cgcttgctgg cgcttatagc gaccatacag cagcaacgat gacatcgcag
     9961 caaacgaaag cagggcggca ggcagcgcca cgtaattata tgccagcccc agcgtcagca
    10021 tcatacctcc gcaatatgcg ccgacggcgc taccgaggtt aaacgctatt tgcccacctg
    10081 cggcacctaa taactctccg cctttggcgt tttgtagtaa caatatttgc agaggtgctg
    10141 aaagggcaaa taattccgcg caacaaataa aagcaaaaat aagcgacgtt gttttcatgc
    10201 cgccgcagaa aaagagcatc agcagtgcca gtacaattat aaagtcagtc actgctgcaa
    10261 tgcgcagtgg tgaataacgt cctgaaatcc tgccacttag catatttccc aacaccatcc
    10321 ctagcccaac taacatcata ataaaggtca tcgccgtttc cgaaaaaccg gaaataaaca
    10381 tcatgtatgg ctttacgtag ctgaaccagg caaacacacc tgcgttgcca aacatcgtgg
    10441 cggcgaaaat taaccacggg gccgggctgc gtaaaaagtg aaattgttcg cgcagctttc
    10501 ctttcgcctc gtcgcgaata tccggcaccc aaaaatagac cgatgccatc accgcaatat
    10561 taaaaacagc gatcaataaa aaggtgtaac gccagctaaa ttcctgactt aaatacgttc
    10621 ccagcggaat gcccagcaaa ttggcgactg tcatcccgga aaccatcccc gccacggcgg
    10681 cggtgacttt tccgggtttg ataatttttg ataacacgat cgcgccgacg ccaaaaaatg
    10741 cgccatgcgg aaagccggaa accaggcgac caatggcaag catcaggtaa gacgaagaga
    10801 gcgtgaacat ggcgttgcca atgacgcaca acgccaccag aaacaacaag atatgtttga
    10861 gtgagtagcg gctggaaaag agtgcgatga ttggcgcacc gaccaccact cccagcgcat
    10921 aatacgagat catatgcccg gcagcaggaa tcgaaattcc tacgttatgc gccagctccg
    10981 tgagcacgcc cataatgcca aattcggcca tccccaaacc aaacgtgccc agagccaaag
    11041 ataaaatgac ttttttcata ccaccatcac aactaccaga acggctacca atcaccgtag
    11101 ccaataaagt gatagcttgc aggataacga ccagcaaggc caagagataa ttcgcccctc
    11161 tgtattcatt atcctgctga atagttattt cactgcaaac gtactttcca gtttgctgta
    11221 gcctaggccg ttgatctttt tcactttgat ctgcaccgga atacgctctt tcatcgcttc
    11281 tacgtggcta atcacaccga tggttttgcc actggcgttc agggcatcca gcgcatcaag
    11341 ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct tcatcaagga acagcgagtc
    11401 aatacgtgtt ttatggctga ccagatccga aagcgccagc gccagcgcca gactaacgag
    11461 gaaactttcg ccgccggaaa gggtacgggt atcgcgtacc gcatctgcct gccaggtatc
    11521 aacaacctcg acttccagcg tctcgctggc tttgcgctgt aacagatagc gcccgtgcag
    11581 ccgggtaagt tgctgattag cgagatggac taaattatcc agcgtcagcc cctgggcaaa
    11641 cttgcggaat ttatcgccct ctttggaacc tattagcgaa ttcagatatc cccagtcctc
    11701 aacctgctgc gtcatttgag caatttgctg cattaaggtt tgttgttgct gacggttatc
    11761 tgcatcctgc ttcagctgct ggcgaatctc gccttgactc gtcgtgtttt cacgcaactt
    11821 ttggtgagtt tgcgctaact cttgctgaat ctgctccacc gtcacagtga gagccaaccc
    11881 gtcaggtcgg tgttgttgat gctgtgccag cgtttctgct gtctgagtga ccagcgtttg
    11941 cgcctgacgg cgctggtttt ccagattctg cttgagctgt tccagctgcg ttagtgtttg
    12001 ttcatccatt agcgccgcaa ggaacgcctg ctgatcgtca aagacgctgg cctgtagcgc
    12061 ggtgtcaaac tgcgcctggg ctttttgcag actttgcgcc gccagaacat cctgttgctg
    12121 taatgtctgc tgctggctgt gtaatgcgag acattgttca tgaacctgcc gccagttatc
    12181 cagcgccaca gtttcttcgc tgtgcgggag atcatcactt tgcggcaacg tttccagaat
    12241 cggcgtcagc tgctgaatac ggttttgcag cgcggttaat tcgttctggc gttgctgcca
    12301 gctctgcgct tcttgctgac gtgtcgccaa ccagctctct tcttcatctt cctgtggcaa
    12361 tgtcagtgca taacccgcca atgccgttaa gagctgctgc tggcgttgtt caatttgctg
    12421 ttgatactgg ataatttgct gattatgcgc ggcaatctgc ccttgtaatt catgtcgttg
    12481 gctgagtaac cgcagctggc gttcgtgctc atcttgtgca tcaagccacg gttgaatatc
    12541 gtcctgtggc tgcaaggtga tattgaggct ggccgtgacg gcttgccatt gttgagtaag
    12601 tgcttgctta tcttgtcgga ggctttgcgc ttcgttttca tcgcgctgaa gctgcttcgt
    12661 taatgcatcc agttgcccgc gcagcgccgc accttcttcg ccgagctttt taacttcgtt
    12721 ttccagcgcc agtaatcgag actgattaac gccaggctcc agcgcctgat acgcctcgac
    12781 cgccggatgg ctggtggaac cacaaagtgg acaaggctga cccgcctgta actgtgcacg
    12841 ctgggcttcc agcgttttga tgcgcgcttc ctgctcgcaa atggttttca catcggcaag
    12901 ttgctgcgtc ttttctttat aacgctggcg catttcgtta agtgcgacat tacgttgcgt
    12961 ctgttccaga gtgacattct ggatagtgac cattaactgc gccagacgtt tttgttgggg
    13021 aacaatctgc ccatgcagcg cgaccaggcg ctgacgcagt gggcgttgct cagcatgttg
    13081 cgccagggcg gtagcaactt catcggcggt taacgtcaac gtgatcgccg caagcgcatt
    13141 aagtttttgc tcagcatggg ttaactgttg ctgccattgc cgcagatgct cgcgatcgct
    13201 ggtttgttgg gagaactgcg cacgccaacc cgccagttcg ttgttccact gacggaagcg
    13261 gtcgtgttcc tgtaaccagg tattcaggct ttgttgctgc tgctgtaatt ctgctgactg
    13321 cttcgccgcg tggtggcgaa tgctcgcgcg aagcgccatt gtgctctgta agcgagtatt
    13381 tacttcttca atccgctggc gaatatgcgc cagcgccgcg ctgtgttctg cgatgcgttc
    13441 ccagtgtgga cgaagatttc gtgccggttg tgccagacta agcgccgcca gttgaggttg
    13501 cgctttttct tcttcggcta acacctgttg caaggcctgc tgacggcggc tggcttcttg
    13561 ctgcaattcg tcctgacgcg ttaaccagtt tagcgattgt tgttcttgct gctgcgcggt
    13621 aagtaactgt ttttcttcgt cagtaagtac ctgcaaactc gctgtcagcg attgcacttg
    13681 ttccggcgtg agcaacgcga cgccgctggc ctgcgcttgc agcttctcca gctctgtgcg
    13741 ggccgatttg tgctgctcaa aaaccatcgc cgagatttgc ccgtagattt cagtgccggt
    13801 taactcctcg agcaattccg cgcgttcttt gggtttggca ttcaggaagg cagcaaattg
    13861 cccctgcgaa agcagcatcg aacgggtgaa gcgcccgtaa tccagcccgg ttaacgtcgc
    13921 tgtcagttcc agcttatctt tcactttgtc ggcgagaatt ttgccgtcgg cgcagcgcgc
    13981 cagctctacg cgtggcacct gcaaattacc gtcgggttgg ttacgcgccc gattctggct
    14041 ccagaatgca cggtacgctt cacctttcac ttcaaactcc acctccgcca gacattcggc
    14101 ggtatcgcgg gtcatgagat cattttgcga ttgtgaaacg ttagagagac gcggagtttc
    14161 atgatacagc gccagacaaa tggcgtccag cagggtggtt ttccccgcac ctgttgggcc
    14221 ggtaatagca aacagcccgt tgctggcgaa cggctcgcgg gtgaaatcaa tcttccattc
    14281 gccttttaat gagttcaggt ttttcaggcg caggctgaga attttcatgc ttcgtgttct
    14341 ccggcgaggg tatgcaacgt cgtggtgaaa agatgctgca gacgttgctg ctgcgattca
    14401 tccagttctt ccagtgccag acggcgattg aacacctctt cgacgctgag ttcgctgagg
    14461 gtttcacgct gttggctggc taacacacgc tcgcgctgtt cacgactccg acgtaccagc
    14521 aatacttcga caggcaatga ttcggttaat gcctggattt tgcgctgaat atcatgcaga
    14581 tactcatcag tagtgatttc gatatccagc cagacaggtg gctcctgcga tacatcgcgc
    14641 cactgttcca gctgtgcggt aatcgacgcc agatcgcctt tcagcactgc catgggttgc
    14701 gttaccggta cgttcaggtt ttccacgctc tctaatttgc cgtttgaaaa tgtcaccaga
    14761 tggacatatt tactcttacc gcattcatca aaactcagtg gaatggggga gccgcaatag
    14821 cgaacatgtt ccatgccgcc aataatctgt gcgcggtgaa tatgcccgag cgcgatgtag
    14881 tcggctggtg gaaagttttg tgccggaaac gcgtccagcg tgccaatata aatgtcacgc
    14941 acggcgtcac ttttactggc ccccacggtc gttaaatgtc ccgtggcgat gatgggcaga
    15001 ggctgatcgc cgcgcagttt gcaggcatcg gcatagtgtt gttggtaata atcggtaatc
    15061 gctgccagta aatgctgctg tttttcaata ccgttaagcc ccgcctggct ggtaataatg
    15121 tcacgtggac gtaaaaacgg aatggggcac agcactgcgc ctggcgtccc gtcgcgacga
    15181 ggcaagattt gcggcgcatg tccggcgctg gcgaccacgg tagtattgag gaacgccatg
    15241 atatcgcgcg attcattcag cgtggcgacc gagtcatggt ttcctgccag taccaccaga
    15301 tgacagccag tttgctgtaa attgacaaca aaacggttgt ataacgtgcg ggcgtaactg
    15361 ggcggcgagc cggtatcgaa aacatcaccg gcaacaataa tcgcatccac ctgatgggtt
    15421 tgtgctgtct ccagcagcca gtcaagaaaa gcctgatgtt cagcttcgcg gcttttactg
    15481 tagaagttct ggccgagatg ccagtctgag gtgtgaagga tgcgcataac ggttccctgg
    15541 cgaaaaagca tgggcgcgat tatacccaaa cagatgtgcc atttgctttt ttctgcgcca
    15601 cggaaatcaa taacctgaag atatgtgcga cgagcttttc ataaatctgt cataaatctg
    15661 acgcataatg acgtcgcatt aatgatcgca acctatttat tacaacaggg caaatcatgg
    15721 cgagacgtat tctggtcgta gaagatgaag ctccaattcg cgaaatggtc tgcttcgtgc
    15781 tcgaacaaaa tggctttcag ccggtcgaag cggaagatta tgacagtgct gtgaatcaac
    15841 tgaatgaacc ctggccggat ttaattctcc ttgactggat gttacctggc ggctccggta
    15901 tccagttcat caaacacctc aagcgcgagt cgatgacccg ggatattcca gtggtgatgt
    15961 tgaccgccag aggggaagaa gaagatcgcg tgcgcggcct tgaaaccggc gcggatgact
    16021 atatcaccaa gccgttttcg ccgaaggagc tggtggcgcg aatcaaagcg gtaatgcgcc
    16081 gtatttcgcc aatggcggtg gaagaggtga ttgagatgca gggattaagt ctcgacccga
    16141 catctcaccg agtgatggcg ggcgaagagc cgctggagat ggggccgaca gaatttaaac
    16201 tgctgcactt ttttatgacg catcctgagc gcgtgtacag ccgcgagcag ctgttaaacc
    16261 acgtctgggg aactaacgtg tatgtggaag accgcacggt cgatgtccac attcgtcgcc
    16321 tgcgtaaagc actggagccc ggcgggcatg accgcatggt gcagaccgtg cgcggtacag
    16381 gatatcgttt ttcaacccgc ttttaacgcc ttgctcatcg gacgcagagc agggcttatg
    16441 atttcttaac tggagtatct tacgtgctgg aacggctgtc gtggaaaagg ctggtgctgg
    16501 agctgctact ttgctgcctc ccggctttca tccagggtgc attttttggt tacctgccct
    16561 ggtttttgct ggcatcggta acgggactgc ttatctggca tttctggaat ttattgcgcc
    16621 tttcatggtg gctgtgggtg gatcgtagta tgaccccgcc accggggcgt ggtagctggg
    16681 aaccgctact atacggctta caccagatgc agctgcgaaa taaaaaacgc cgccgtgaac
    16741 tgggcaatct gattaaacgc tttcgtagcg gcgcggagtc gctgcccgac gcagtggtgc
    16801 tgaccacgga agagggcggt attttctggt gtaacggtct ggcgcaacaa attcttggtt
    16861 tgcgctggcc ggaagataac gggcagaaca tccttaacct gctgcgttac ccggagttta
    16921 cgcaatatct gaaaacgcgt gatttttctc gcccgctcaa tctggtgctc aacaccgggc
    16981 ggcatctgga aattcgcgtc atgccttata cccacaaaca gttgctgatg gtggcgcgtg
    17041 atgtcacgca aatgcatcaa ctggaagggg cgcggcgtaa cttttttgcc aacgtgagcc
    17101 atgagttacg tacgccattg accgtgttac agggttacct ggagatgatg gatgagcagc
    17161 cgctggaagg cgcggtacgc gaaaaagcgt tgcacaccat gcgcgagcag acccagcgga
    17221 tggaagggct ggtgaagcag ttgctaacgc tgtcgaaaat tgaagccgcg ccgacgcaat
    17281 tgctcaatga aaaggttgat gtgccgatga tgctgcgcgt tgttgagcgc gaggctcaga
    17341 ctctgagtca gaaaaaacag acatttacct ttgagataga taacggcctc aaggtgtctg
    17401 gcaacgaaga tcagctacgc agtgcgattt cgaacctggt ctataacgcc gtgaatcata
    17461 cgccggaagg cacgcatatc accgtacgct ggctgcgagt gccgcacggt gccgaattta
    17521 gcgttgaaga taacggaccg ggcattgcac cggagcatat tccgcgcctg accgagcgtt
    17581 tttatcgcgt tgataaagcg cgttcccggc aaaccggcgg tagcggatta gggttagcga
    17641 tcgtgaaaca tgcggtgaat catcacgaaa gtcgcctgaa tattgagagt acagtaggaa
    17701 aaggaacacg tttcagtttt gttatcccgg aacgtttaat tgccaaaaac agcgattaat
    17761 ccgcctttgt catcttttat tgccataagc cagccgatgc tggcttattt tctttgcagt
    17821 caaaatacgg gcgttagatt ttacaacggt tggtgatttt ttgttcgcat gattagccat
    17881 gtctttttca cggaaatagt gttttatact ggttggtgat ttcttatcgc tatatacctc
    17941 tggtttttag atccctcctt gctttaaaac gttataagcg tttaaattgc gcttcaggtg
    18001 ctgtcatact gactgcatta acgcggtaaa tcgaaaaact attcttcgcc gcgcctggtt
    18061 gggagtattt cccgctaaaa ttgtttaaat ataccgctgt atcatcccca gggattggca
    18121 caaaaattta acgttacaac accacatcca caggcagtat gatttatgac ccatcaatta
    18181 agatcgcgcg atatcatcgc tctgggcttt atgacatttg cgttgttcgt cggcgcaggt
    18241 aacattattt tccctccaat ggtcggctta caggcaggcg aacacgtctg gactgcggca
    18301 ttcggcttcc tcattactgc cgttggcctg ccggtattaa cggtagtggc gctggcaaaa
    18361 gttggcggcg gtgttgacag cctcagcacg ccaatcggta aagtcgctgg cgtactgctg
    18421 gcaacggttt gttacctggc ggtggggccg cttttcgcta cgccgcgtac agctaccgtt
    18481 tcctttgaag tggggattgc gccgctgacg ggtgattccg cgctgccgct gtttatctac
    18541 agcctggtct atttcgctat cgttattctg gtttcgctct atccgggcaa gctgctggat
    18601 accgtgggca acttccttgc gccgctgaaa attatcgcgc tggtcatcct gtctgttgcc
    18661 gcgattgtct ggccggcggg ttctatcagc acggcgactg aggcttatca aaacgctgcg
    18721 ttttctaacg gcttcgttaa cggctatctg accatggata cgctgggcgc aatggtgttt
    18781 ggtatcgtta ttgttaacgc ggcgcgttct cgtggcgtta ccgaagcgcg tctgctgacc
    18841 cgttataccg tctgggctgg cctgatggcg ggtgttggtc tgactctgct gtacctggcg
    18901 ctgttccgtc tgggttcaga cagcgcgtcg ctggtcgatc agtctgcaaa cggcgctgct
    18961 attctgcatg cttacgttca gcacaccttt ggcggcggcg gtagcttcct gctggcggcg
    19021 ttaatcttca tcgcctgcct ggtaacggca gttggcctga cctgtgcttg tgcagaattc
    19081 tttgcccagt acgtaccgct ctcttatcgt acgctggtgt ttatcctcgg cggcttctcg
    19141 atggtggttt ctaacctcgg cttaagccag ctgatccaga tctccgtacc ggtgctgacc
    19201 gctatttatc cgccgtgtat cgcactggtt gtattaagtt ttacacgctc atggtggcat
    19261 aattcgtccc gcgtgattgc tccgccgatg tttatcagcc tgctttttgg tattctcgac
    19321 gggatcaaag catctgcatt cagcgatatc ttaccgtcct gggcgcagcg tttaccgctg
    19381 gccgaacaag gtctggcgtg gttaatgcca acagtggtga tggtggttct ggccattatc
    19441 tgggatcgcg cggcaggtcg tcaggtgacc tccagcgctc actaaatcac tgaacatttg
    19501 ttttaaccac ggggctgcga tgccccgtgg ttttttattg tgttgatggg ttaggaattg
    19561 atggaaagta agaacaagct aaagcgtggg ctaagtaccc gccacatacg ctttatggca
    19621 ctgggttcag caattggcac cgggctgttt tacggttcgg cagacgccat caaaatggcc
    19681 ggtccgagcg tgttgttggc ctatattatc ggtggtatcg cggcgtatat cattatgcgt
    19741 gcgctggggg aaatgtcggt acataacccg gcagccagct ctttctcgcg ttatgcgcag
    19801 gaaaacctcg gcccgctggc aggttacatt accggctgga cctattgctt tgaaatcctt
    19861 attgtcgcca tcgccgatgt gaccgctttt ggtatctata tgggtgtctg gttcccgacg
    19921 gtgccgcact ggatttgggt actgagcgtg gtgctgatca tttgcgccgt aaacctgatg
    19981 agcgtgaagg tcttcggtga gctggaattc tggttctcgt tctttaaagt cgccaccatc
    20041 atcatcatga ttgtcgccgg tttcggcatc atcatctggg ggattggcaa cggcgggcaa
    20101 ccgaccggta ttcataacct gtggagcaac ggcggcttct tcagtaacgg ctggcttggg
    20161 atggtgatgt cgttgcaaat ggtgatgttt gcttacggtg ggatcgaaat tatcgggatt
    20221 accgccggtg aagcgaaaga tcctgagaaa tcgataccgc gtgcgattaa ctccgtgccg
    20281 atgcgtattc tggtgttcta cgtcggtacg ctgttcgtca ttatgtctat ctacccgtgg
    20341 aatcaggttg gcactgccgg tagcccgttc gtgctgacgt tccagcatat gggcattacc
    20401 tttgccgcca gcattcttaa ctttgttgtg ctgactgctt cgctgtcggc aattaacagt
    20461 gacgtatttg gcgtaggccg tatgctccac ggtatggcag agcagggcag cgcgccgaaa
    20521 attttcagca aaacgtcgcg tcgcggtatt ccgtgggtta cggtgctggt gatgactacc
    20581 gcgctgctgt ttgcggtgta tctgaactac atcatgccgg aaaacgtctt cctggtgatc
    20641 gcttcgctgg caaccttcgc cacggtgtgg gtgtggatta tgatcctgct gtcgcaaatt
    20701 gccttccgtc gccgtttgcc gccagaagaa gttaaggcgc tgaaatttaa agtgccgggt
    20761 ggggtagcaa cgaccatcgg cgggctgatt ttcctgctct ttattatcgg gttgattggt
    20821 tatcacccgg atacgcgtat ctcgctgtat gtcggtttcg cgtggattgt tgtgctgttg
    20881 attggctgga tgtttaaacg ccgccacgat cgtcagctgg ctgaaaacca ataatacttg
    20941 cgttctgtaa gccggttcag gaggaaatcc tgatccggct attccgaaag ttatccgccc
    21001 ccgtcctcct cccccaaata tccttcagat gatgagtgat cctgcattag gctatggcaa
    21061 ggtgatcaga ttttcatcac aggggaatta tgatgttaaa tgcatggcac ctgccggtgc
    21121 ccccatttgt taaacaaagc aaagatcaac tgctcattac actgtggctg acgggcgaag
    21181 acccaccgca gcgcattatg ctgcgtacag aacacgataa cgaagaaatg tcagtatcga
    21241 tgcataagca gcgcagtcag cagcagcctg gcgtcaccgc atggcgtgcg gcgattgatc
    21301 tctccagcgg acaaccccgg cggcgttaca gtttcaaact gctgtggcac gatcgccagc
    21361 gttggtttac accgcagggc ttcagccgaa tgccgccggc acgactggag cagtttgccg
    21421 tcgatgtacc ggatatcggc ccacaatggg ctgcggatca gattttttat cagatcttcc
    21481 ctgatcgttt tgcgcgtagt cttcctcgtg aagctgaaca ggatcatgtc tattaccatc
    21541 atgcagccgg acaagagatc atcttgcgtg actgggatga accggtcacg gcgcaggcgg
    21601 gcggatcaac gttctatggc ggcgatctgg acgggataag cgaaaaactg ccgtatctga
    21661 aaaagcttgg cgtgacggcg ctgtatctca atccggtgtt taaagctccc agcgtacata
    21721 aatacgatac cgaggattat cgccatgtcg atccgcagtt tgacggtgat ggggcgttgc
    21781 tgcgcttacg ccacaatacg cagcagctgg gaatgcggct ggtgctggat ggcgtgttta
    21841 accacagtgg cgattcccat gcctggtttg acaggcacaa tcgtggcacg gggggagctt
    21901 gtcacaaccc tgaatcgccc tggcgcgact ggtactcgtt tagtgatgat ggcacggcgc
    21961 tcgactggct tggctatgcc agcttgccga agctggatta tcagtcggaa agtctggtga
    22021 atgaaattta tcgcggggaa gacagtattg tccgccattg gctgaaagcg ccgtggagta
    22081 tggacggctg gcggctggat gtggtgcata tgctggggga ggcgggtggg gcgcgcaata
    22141 atatgcagca cgttgctggg atcaccgaag cggcgaaaga aacccagccg gaagcgtata
    22201 ttgtcggcga acattttggc gatgcacggc aatggttaca ggccgatgtg gaagatgccg
    22261 ccatgaacta tcgtggcttc acattcccgt tgtggggatt tcttgccaat accgatatct
    22321 cttacgatcc gcagcaaatt gatgcccaaa cctgtatggc ctggatggat aattaccgcg
    22381 cagggctttc tcatcaacaa caattacgta tgtttaatca gctcgacagc cacgatactg
    22441 cgcgatttaa aacgctgctc ggtcgggata ttgcgcgcct gccgctggcg gtggtctggc
    22501 tgttcacctg gcctggtgta ccgtgcattt attacggtga tgaagtagga ctggatggca
    22561 aaaacgatcc gttttgccgt aaaccgttcc cctggcaggt ggaaaagcag gatacggcgt
    22621 tattcgcgct gtaccagcga atgattgcgc tgcgtaagaa aagtcaggcg ctacgtcatg
    22681 gcggctgtca ggtgctgtat gcggaagata acgtggtggt atttgtccgc gtgctgaatc
    22741 agcagcgtgt actggtggca atcaaccgtg gcgaagcttg tgaagtggtg ctacccgcgt
    22801 caccgttgct caatgccgtg caatggcaat gcaaagaagg gcataggcaa ctgactgacg
    22861 ggattctggc tttgcctgcc atttcggcta cggtatggat gaactaacgt tttataacgc
    22921 cttgtgtgac gcctgcgcca tcatccgcgg ataaaactgc cagaagcggg tttcgagggc
    22981 gtcataatga gcgtctaaat cgtaccagga gtcacgcagg gcatccagac gtgggcggcg
    23041 gcttgccatg ccgtttaaca cgttctggat gaaatccata tcgcgatagc gcaccagcca
    23101 ctgctctgac cacaagtaat tgttcagatt gataaaacgt ggcggtgagt ccggcaaaat
    23161 cgtcatcact tgctcgcggg cataacagac aaattcctgt agcggaaagt ccggcgacag
    23221 ctgcgaccag tggcgggaaa gaaagtgatc ccacatgaca tccagcgtaa taggcgcaac
    23281 gcggcgagtt tcactacgaa accattcccg tgcttcgcgg acttccggca gattgtcggt
    23341 taatacgtcg atacgtcgat gcatatgaat gccagccacg acgtcgggcg gaaaactttc
    23401 ttcaggattt ccgcgtacga aatcagccag taaattgccg gaaagcgagc tttccgcgag
    23461 atgggctaaa tgcaggtgag ctaaaaaatt catcgattat attctatcca aaagggggta
    23521 aaggttgcag ggagagcgcc ccggcactag actacccgcc tcttatttta gtctgagtca
    23581 gtgtcatgcg cgttaccgat ttctcctttg aattgcccga atccctgatt gcccactatc
    23641 ccatgcctga acgcagtagc tgtcgtttac tgtcgctgga cgggccgacg ggcgcgctga
    23701 cgcacggtac tttcaccgat ttacttgata agctcaaccc cggcgatctt ctggttttta
    23761 ataatacccg cgtgatcccg gcgcgcctgt ttgggcgtaa agccagcggc ggcaagattg
    23821 aagtgctggt tgaacggatg ctcgacgaca aacgcattct tgcgcatatt cgcgcctcga
    23881 aagcgccaaa acctggcgca gaactgctgc tgggcgatga cgaaagtatt aacgcaacaa
    23941 tgaccgcgcg ccacggcgca ctgtttgaag tcgaatttaa tgatgaacgc tcggtgctgg
    24001 atattctcaa cagcatcggc catatgccgc tgccgccgta tatcgaccgt ccggacgaag
    24061 acgctgaccg cgaactttat caaaccgttt atagcgaaaa accgggcgcg gttgcagccc
    24121 cgaccgcagg tctgcatttt gacgaacctt tgctggaaaa attacgcgcc aaaggcgtgg
    24181 agatggcgtt tgtgacgttg cacgttggtg cgggcacctt ccagccggtg cgcgtcgaca
    24241 ccattgaaga tcacatcata cactcggaat acgctgaagt gccgcaggat gtggtagacg
    24301 cggtactggc ggcgaaagcg cgcggtaacc gggtgattgc ggttggcacc acctcagtac
    24361 gttcgctgga aagcgcggct caggcggcga aaaacgatct tatcgaaccg ttctttgacg
    24421 atacccaaat ctttatctat ccgggcttcc agtacaaagt ggtcgatgcg ctggtgacaa
    24481 acttccacct gccggaatcg acgctgatta tgctggtttc ggcctttgcc ggttatcaac
    24541 acaccatgaa cgcctataaa gcagcggtag aagagaaata tcgctttttt agttacggtg
    24601 atgcgatgtt tatcacgtac aatccgcagg caattaatga gcgcgtcggg gagtaattcc
    24661 gcggcgctgg tttaaaacgt tggactgttt ttctgacgta gtggagaaaa aatgaaattt
    24721 gaactggaca ccaccgacgg tcgcgcacgc cgtggccgcc tggtctttga tcgtggcgta
    24781 gtggaaacgc cttgttttat gcctgttggc acctacggca ccgtaaaagg gatgacgccg
    24841 gaagaagttg aagccactgg cgcgcaaatt atcctcggca acaccttcca cctgtggctg
    24901 cgcccgggcc aggaaatcat gaaactgcac ggcgatctgc acgattttat gcagtggaag
    24961 gggccgatcc tcaccgactc cggcggcttc caggtcttca gccttggcga tattcgtaaa
    25021 atcaccgaac agggcgtgca cttccgtaac ccgatcaacg gcgatccgat tttcctcgat
    25081 cctgaaaaat caatggagat tcagtacgat cttggttcgg atatcgtcat gatctttgat
    25141 gagtgtacgc cgtatcctgc tgactgggat tacgcaaaac gctccatgga gatgtctctg
    25201 cgttgggcga agcgtagccg tgagcgtttt gacagtctcg gaaacaaaaa tgcgctgttt
    25261 ggtatcattc agggcagcgt ttacgaagat ttacgtgata tttctgttaa aggtctggta
    25321 gatatcggtt ttgatggcta cgctgtcggc ggtctggctg tgggtgagcc gaaagcagat
    25381 atgcaccgca ttctggagca tgtatgcccg caaattccgg cagacaaacc gcgttacctg
    25441 atgggcgttg gtaaaccaga agacctggtt gaaggcgtac gtcgtggtat cgatatgttt
    25501 gactgcgtaa tgccaacccg caacgcccga aatggtcatt tgttcgtgac cgatggcgtg
    25561 gtgaaaatcc gcaatgcgaa gtataagagc gatactggcc cactcgatcc tgagtgtgat
    25621 tgctacacct gtcgcaatta ttcacgcgct tacttgcatc atcttgaccg ttgcaacgaa
    25681 atattaggcg cgcgactcaa caccattcat aaccttcgtt actaccagcg tttgatggcg
    25741 ggtttacgca aggctattga agagggtaaa ttagagagct tcgtaactga tttttaccag
    25801 cgtcaggggc gagaagtacc acctttgaac gttgattaat attaataatg agggaaattt
    25861 aatgagcttt tttatttctg atgcggtagc ggcaacgggt gcaccggcgc aaggtagccc
    25921 gatgtctttg attttgatgc tggtggtatt cggtctgatt ttctatttca tgatcctgcg
    25981 tccacagcag aagcgcacca aagaacacaa aaagctgatg gactccattg ccaaaggtga
    26041 tgaagttctg acgaacggtg gcctggttgg tcgcgtaacc aaagtagcgg aaaacggcta
    26101 cattgctatc gcgctgaatg acaccactga agtagttatt aaacgtgact tcgtagctgc
    26161 cgtcctgccg aaaggcacca tgaaggcgct gtaattaaaa tttttcccta agggaattgc
    26221 cgtgttaaac cgttatcctt tgtggaagta cgtcatgctg atcgtggtga ttgtcatcgg
    26281 tctgctgtat gcgcttccca acctgtttgg tgaggatccg gctgttcaga tcactggtgc
    26341 gcgcggagtc gccgccagtg agcaaacgct gatccaggtc cagaaaacgt tacaagaaga
    26401 aaaaataact gctaagtctg tggcactgga agagggcgct attcttgcgc gcttcgactc
    26461 cactgacacc cagctgcgcg ctcgtgaagc attaatgggc gttatgggtg acaaatacgt
    26521 cgtggcgctt aaccttgccc cggcaacgcc gcgctggctg gcagctattc acgctgagcc
    26581 gatgaagctc ggccttgacc tgcgtggcgg cgttcacttc ctgatggaag tggatatgga
    26641 caccgcgctt ggcaaactcc aggaacaaaa tatcgatagc ctgcgcagcg atctgcgtga
    26701 aaagggcatc ccgtatacca ccgttcgtaa agaaaacaac tacggcctga gcattacttt
    26761 ccgcgatgct aaagctcgtg atgaagccat tgcgtatctg agcaagcgcc atccggacct
    26821 ggtgattagt agccagggca gcaaccagct gcgtgcggta atgagcgatg ctcgtctgag
    26881 tgaagcgcgt gaatatgcgg tgcagcagaa catcaatatc ctgcgtaacc gtgtaaacca
    26941 acttggcgtg gcggagccgg tggttcagcg tcagggtgct gaccgtatcg ttgttgaact
    27001 gccaggtatt caggacactg cgcgtgcgaa agagattctg ggtgcgacgg caacgctgga
    27061 attccgtctg gtaaacacca acgttgacca ggccgctgcg gcatctggtc gcgtaccggg
    27121 cgactctgaa gtgaaacaga cccgcgaagg tcagccagtt gtgctgtaca aacgcgtaat
    27181 tctgaccggt gaccatatca ccgactccac ttccagccag gacgaataca accaaccgca
    27241 ggttaacatc tcgctcgata gcgctggtgg taacatcatg tctaacttca ctaaggacaa
    27301 catcggcaaa ccgatggcaa ccctgtttgt ggagtacaaa gacagcggta agaaagatgc
    27361 aaatggtcgt gcggttctgg tgaaacagga agaggtgatt aacatcgcca acatccagtc
    27421 tcgtctgggt aacagcttcc gtatcaccgg catcaacaac ccgaacgaag cccgtcagct
    27481 gtcactgctg ctgcgtgccg gtgcgttgat cgcgccgatt cagattgttg aagaacgtac
    27541 cattggccca accctgggta tgcagaacat tgaacagggg ctggaggctt gccttgccgg
    27601 tctgctggtt tctattctgt tcatgatcat cttctataag aagtttggtc tgattgcgac
    27661 cagtgctctg attgccaact tgatcttaat cgtcggcatt atgtcgctgt tgccaggcgc
    27721 aacgctgagt atgccaggta tcgcgggtat cgtcttaacc cttgcggtgg cggtcgatgc
    27781 gaacgtactg atcaacgaac gtattaaaga agagttgagc aacggacgta ctgttcaaca
    27841 ggcaattgat gaaggttatc gtggcgcatt cagttctatc ttcgatgcga acatcaccac
    27901 gctgattaaa gtcatcatcc tgtacgcagt gggtaccggg gcaattaaag ggttcgcgat
    27961 tactaccggt atcggtgtgg cgacgtcgat gtttaccgcg attgtcggta cgcgtgccat
    28021 cgtaaacctg ctatatggcg gcaagcgcgt caagaagctg tcaatctgag gagtgcgatg
    28081 tggcacagga atatactgtt gaacaactaa accacggccg taaagtctat gactttatgc
    28141 gctgggacta ctgggctttc ggcatctctg gtctgctgtt aatcgccgct atcgttatta
    28201 tgggcgtgcg cggctttaac tgggggctgg atttcaccgg tggtacggtt atcgaaatta
    28261 cgctcgaaaa accggctgaa attgacgtaa tgcgtgatgc attgcaaaaa gccggttttg
    28321 aagagccaat gctgcaaaac tttggtagca gccacgacat catggtccgt atgccgcctg
    28381 ctgaaggcga aaccggcggt caggtgctgg gcagccaggt tctgaaggtg attaacgaat
    28441 ccaccaatca gaatgcagca gtgaagcgta ttgagttcgt cggtccgagc gtgggggcag
    28501 accttgcgca aaccggtgcg atggcgttga tggcggcgct gctgtctatc cttgtgtacg
    28561 taggttttcg ctttgagtgg cgactggcgg caggggtggt tattgcgctg gcgcacgacg
    28621 ttatcattac gctgggtatt ttgtcgttat tccatatcga gattgacctg accattgtgg
    28681 catcgttgat gtcggttatc ggttactcgc ttaacgacag tatcgtggta tcggaccgta
    28741 ttcgtgaaaa cttccgcaag atccgtcgcg gtacgcctta cgaaatcttt aacgtgtcct
    28801 tgacccagac gctgcaccgt accttgatca catccggtac taccttgatg gttatcctga
    28861 tgctgtacct cttcggtggt ccggtactgg aaggcttctc gctgaccatg cttatcggtg
    28921 tttccatcgg tactgcatct tccatctatg tggcatctgc gttggctctg aaactgggta
    28981 tgaagcgcga acacatgttg cagcagaaag tggaaaaaga aggggcggat cagccgtcaa
    29041 ttctgccgta atcaagttcc cgttgatgtt gaaaatcccg gtcagaagat cgggattttt
    29101 tttgatgtat ggagattgcg aaaatccccg catcttggga aactgcgcgt aaccctacat
    29161 ttcatccagg taactcttca tggctatcat cccaaaaaac tatgcgcggt tagaaagcgg
    29221 ctatcgcgaa aaagcattaa aaatatatct gtgggtctgc ggtcgctgtt cccgcgagtt
    29281 tgtttattcc aacctgcgtg aacttactgt tcaccacatt gatcacgacc ataccaataa
    29341 cccggaagat ggcagtaact gggaattgct ttgtctctat tgccacgatc atgagcattc
    29401 gaaatatacc gaagcggatc agtatggtac gaccgttatc gcaggggaag atgcgcagaa
    29461 agatgtcggt gaagcgaagt acaacccatt cgctgacctg aaagcgatga tgaacaagaa
    29521 gaagtgatta aaacgtaaaa ttgcctgatg cgctacgctt atcaggacta cgttatttca
    29581 gcaatatatt gaatttacgt gcttttgtgg gtcggacaaa gcgtttacgc cgcatccggc
    29641 atgaacaaag cgcacgttgt taacaatctg aaatgccggg aataaatccc ggcattttca
    29701 taatcagaag ttgtaaccta caaccaggta accaccccag ccggtagagc gaacgttgaa
    29761 gttgccgttg ccgaagttca gttctgcatc gtcgttccac tgaccaccgt cgtgccagta
    29821 acgagctacg acagagtagt gccagtgatc gtagttcaga gccagaatat ggctggaagc
    29881 gatagagtta ttagtacggg tcttaatacc gttgattgcg ttaccgctgt catcccctaa
    29941 atcggaaccc cagtcgaagt tggtgaagcc gatgtagctc agctgaccgc cccacagatc
    30001 ggtaatcggc acaaagtatt taattttgaa acggtaaccg tcccactcgt tttcgtttgc
    30061 tgcgccatag ttctgccact ggtatttcgc atagacgttc atggacaggc tcatcggcag
    30121 accagtatcg atgtcggtac ccagacccat gtaccaggtg ctctggcgac catctttatt
    30181 acgacccatg tcgtaaatgt agttgttcgc gaagtaccac tctttgaacg gaccgaagct
    30241 aaggtcagta ttggtcagct tgtcgatgga gaaacgtggt tcgatttcca taaacagcgg
    30301 agaaccgtgg ttccagatac ctttagcatc ggagttaccg ccgaagaata ccggcgcatc
    30361 cgcataacca tagaagtcga accagtcttt tttagcgaat gcttcgtact caaggtaggt
    30421 atcgttgcgg atctgcggtc cgaaacgggt gtgatagctt ccgacaacgt taacgctctg
    30481 gtgccaccag tcggaaagat actgcggttt gtcgttttca gctgcgttga cagtaaaaga
    30541 cgaagagagc gccagtaccg caccggctgc cagtaatgtt tttttcatat gtatgccact
    30601 gtttgaaaat cccttgcggg agtgaaaaag gcgcaaattg cgtttctaaa tatttcgtat
    30661 acagaggagc ctattataaa aatcattgct cacaaaaata tgtttcgttt cacagttcta
    30721 tcatttacgt aatcgattgc gttcacgttt acacacattc gggcagggat tgtactgact
    30781 ttcacccttg ttgcaaactt ttactatcaa tcagggtgat gtaagcggag taacaaaatg
    30841 acggggaaag atgttgcgag acgcacaaaa tcgccccgaa atacgtttac tgcataacag
    30901 gttgaatatc gtggatacgt acgaagtcta actgttctac agaaataccc tttaactgta
    30961 acggaatatc aacatcgctg ggggcgagga cgctggcagg ggcattaatc aattgattct
    31021 gcacattgat ttcctgaaag ttgtctattg ttccctgaat ttgcccatat tcaacagttc
    31081 cgctgaatgc gggcaaggga tcattcgact cgccctgaat acgtagcgtc agggtggtgc
    31141 catctgcatc aggcgtaata ttcaccagcg acatacgtaa agtaccgatc tggctttcca
    31201 gtcttgccgg tgttttcgct cctggcagaa ggtaaacccc actgctggat tttgcattca
    31261 gcctgttttg ctgagtgatt ttgaccgttt ctttattgag ttgcgtcatc tcttggttca
    31321 gcgtgctgac gctgtgtttc atctgtcgca cttcgctctg ctgcacacag gcgctaaggc
    31381 tgaacaggct tcccagcagg agcagtcgaa aaacgttagt attcataggt cgtccctttc
    31441 tcgtcgcaat tagcctaatg gtagagggta aaagcagcga aagcatcgat cctttatctc
    31501 aaaagcgttg cgcctttgtt gtatcgtcag ttcagggtaa aatagatttc tgttaaccac
    31561 ctagtcagga cgccgtatgc attgcccatt ctgtttcgcc gtggacacta aggtaattga
    31621 ctctcgtctc gtgggcgagg gttcatccgt acgccgccgt cggcagtgtc tggtgtgtaa
    31681 tgaacgtttc accacctttg aagtggcgga gctggttatg ccgcgtgttg taaaaagcaa
    31741 cgacgtgcgt gaaccgttta atgaagagaa attgcgtagc ggaatgctgc gggcgctgga
    31801 aaaacgtccg gtgagttccg atgacgtcga aatggcaatc aatcatatta aatcgcagct
    31861 gcgcgccacc ggtgagcgcg aagtgccgag caagatgatt ggcaatctgg tgatggagca
    31921 attgaaaaag ctcgataaag tcgcctatat ccgttttgcc tctgtctacc gcagtttcga
    31981 agatatcaaa gaatttggcg aagagatcgc gcgcctggag gactaagccg tgcaggacga
    32041 gtattacatg gcgcgagcgc taaagctggc gcaacgagga cgttttacca cgcatcccaa
    32101 cccgaatgtc gggtgcgtca ttgtcaaaga tggcgaaatt gtcggtgaag gttaccacca
    32161 acgtgcgggt gaaccacatg ccgaagtaca cgcgttgcgt atggcgggtg aaaaagccaa
    32221 aggtgcgacc gcctatgtca cactcgaacc ctgtagccat catggtcgta cgccaccgtg
    32281 ctgtgacgca ctcatcgccg ctggcgtagc gcgcgtggtt gcctcgatgc aagatcctaa
    32341 cccgcaggtc gctgggcgtg gactttaccg tctgcaacag gctggcattg acgtcagcca
    32401 cgggctgatg atgagtgaag ccgagcaatt gaataaaggc tttctcaagc ggatgcgcac
    32461 cggctttcct tatattcagt taaaacttgg cgcatcgctt gatggccgca cggcgatggc
    32521 gagcggcgaa agccagtgga tcacttcgcc tcaggcgcgg cgcgacgtac aacgacagcg
    32581 cgcacaaagt catgccattt taaccagcag cgccacggtg ctggcggatg atccagcctt
    32641 aacagtgcgt tggtctgaac tggatgaaca aactcaggcg ctctacccgc aacaaaatct
    32701 ccgccagcca gtacgtattg tgattgatag ccaaaatcgc gtgacgccgg aacatcgcat
    32761 tgtgcagcag cccggcgaaa cctggttcgc gcgtacgcag gaagattcta gtgagtggcc
    32821 ggaaacggtg cgtaccttgc tgattccaga gcataaaggt catctggatc tggttgtact
    32881 gatgatgcaa ctgggtaaac agcaaattaa cagtatctgg gtggaagcgg ggccaacgct
    32941 cgctggcgca ttgctgcagg cgggtttagt cgatgagctg attgtctata tcgcacctaa
    33001 actattaggc agcgacgccc gcggattatg cacgctgcca gggcttgaga aattagccga
    33061 cgccccccaa tttaaattca aagagatacg tcatgtaggc ccggatgttt gcctgcattt
    33121 agtgggtgca tgatctcccg gctcgaaagg gaagcagcgc acgaaatatt atgctaaaat
    33181 ccgcccccct gcggggccat actcgaaccc gaaggaagaa aatgaacatt attgaagcta
    33241 acgttgctac cccggacgct cgcgtcgcca tcaccatcgc gcgtttcaac aactttatca
    33301 atgacagcct gctggaaggt gcaattgacg cactgaaacg tatcggtcag gtaaaagatg
    33361 aaaacattac cgttgtttgg gtgcctggtg cctatgagct gccgctggcg gcgggtgcac
    33421 tggctaaaac cggtaaatac gacgcggtga ttgcgctggg tacggttatt cgtggtggca
    33481 ctgcccactt tgaatatgtc gctggtggtg caagcaacgg cctggcgcat gttgcccagg
    33541 acagcgaaat tccggttgct tttggggttc tgaccactga aagcattgaa caagcgatcg
    33601 aacgtgctgg caccaaagct ggcaacaaag gtgcagaagc tgcactgacc gcgcttgaaa
    33661 tgattaatgt attgaaagcc atcaaggcct gaaattagta aggggaaatc cgtgaaacct
    33721 gctgctcgtc gccgcgctcg tgagtgtgcc gtccaggcgc tctactcctg gcagttgtcc
    33781 cagaacgaca tcgctgatgt tgaataccag ttcctggctg aacaggatgt aaaagacgtt
    33841 gacgtcctgt acttccgtga gctgctggcc ggggtggcga ctaacaccgc atacctcgac
    33901 ggactgatga agccatacct gtcccgcctg ctggaagaac tgggacaggt agaaaaagca
    33961 gtactgcgca ttgcgctgta cgagctgtct aaacgtagcg atgtgccata caaagtggcc
    34021 attaacgaag cgatcgaact ggcgaaatcg ttcggtgcag aagacagcca taagttcgtc
    34081 aacggcgtac tcgataaagc agcacctgtg attcgcccta acaaaaagtg atatccaggc
    34141 cggtagattc acggaagacc gttccgcgat cgccggcctt ttctttttta cctgctgagg
    34201 cataacgtat ggcatgtggc gagttctccc tgattgcccg ttattttgac cgtgtaagaa
    34261 gttctcgtct tgatgtcgaa ctgggcatcg gcgacgattg cgctcttctc aatatccccg
    34321 agaaacagac cctggcgatc agcactgata cgctggtggc gggcaaccac ttcctccatg
    34381 atatcgatcc tgctgatctg gcgtataaag cactggcagt gaacctaagc gatctggcag
    34441 cgatgggggc cgatccggcc tggctgacgc tggcattaac cttaccggac gtagacgaag
    34501 cgtggcttga gtccttcagc gacagtttgt ttgatcttct caattattac gatatgcaac
    34561 tcattggcgg cgataccacg cgtgggccat tatcaatgac gttgggtatc cacggctttg
    34621 ttccgatggg acgagcctta acgcgctctg gggcgaaacc gggtgactgg atctatgtga
    34681 ccggtacacc gggcgatagc gccgccgggc tggcgatttt gcaaaaccgt ttgcaggttg
    34741 ccgatgctaa agatgctgac tacttgatca aacgtcatct ccgtccatcg ccgcgtattt
    34801 tacaggggca ggcactgcgc gatctggcaa attcagctat cgatctctct gacggtctga
    34861 tttccgatct cgggcatatc gtgaaagcca gcgactgcgg cgcacgtatt gacctggcat
    34921 tgctgccgtt ttctgatgcg ctttctcgcc atgttgaacc ggaacaggcg ctgcgctggg
    34981 cgctctctgg cggtgaagat tacgagttgt gtttcactgt gccggaactg aaccgtggcg
    35041 cgctggatgt ggctctcgga cacctgggcg taccgtttac ctgtatcggg caaatgaccg
    35101 ccgatatcga agggctttgt tttattcgtg acggcgaacc tgtcacgttt gactggaaag
    35161 gatatgacca ttttgccacg ccataaagat gtcgcgaaaa gtcgcctgaa gatgagtaat
    35221 ccgtggcatc tgcttgctgt cggattcgga agtggattaa gctcaatcgt tcctggcacg
    35281 atgggatcgc tggcagcgat tccgttctgg tatctgatga cctttttacc ctggcagctc
    35341 tactcgctgg tggtgatgtt ggggatctgt atcggcgtct atctttgtca tcaaacggcg
    35401 aaagatatgg gcgtgcacga tcacggcagc attgtctggg acgaatttat tggtatgtgg
    35461 atcacgctca tggcgctgcc gaccaatgac tggcagtggg ttgccgccgg gtttgtgatt
    35521 ttccgtattc tggatatgtg gaagccgtgg ccgatccgct ggtttgatcg caatgtacat
    35581 ggcggcatgg ggatcatgat cgacgatatt gtcgccgggg tgatttccgc aggcatcctg
    35641 tactttatcg gtcatcactg gccgctgggt attctgtcgt agttgtgcac cgatgcctga
    35701 tgtgacgctt gtcacgtctc atcaggcctg gactcttatt taaatcctac gaccgcatgc
    35761 ggtttatacg gcgtttccag ttcggcaatc tgttccggct tcaaagtgat atccaccgcg
    35821 ttcaatagct catcaagctg ttcttcgcgc gaagttccga taatcggtgc ggcaatgccc
    35881 ggtttactca acaaccaggc cagcgcaact tgtgctcgtg ttgcaccgag ttcttcgctg
    35941 acgcctgtta accgctctgc gatctgcgcg tcattttcat cgctttcttt atagagattt
    36001 ttccccacct catcagacac cagtcgtgcg gtagtttctc cccacggacg cgtcagtcgc
    36061 ccccgcgcca gcgggctcca cggaatcacc gccacgccct cctgataaca cagtggcagc
    36121 atctcgcgct cttcttcgcg ataaatcaga ttgtagtgat cctgcatact gacaaactgc
    36181 gcccagccgt gctgtttttg tagcttcagg gcctgagcaa actgcgaagc gtgcatggat
    36241 gacgcgccga tataacgcgc tttcccggct tttaccacgt cgttcagggc ttccagcgtc
    36301 tcttcgatcg gcgtgttgta atcccagcga tgaatttgca ggatatcgac ataatccatg
    36361 ccgagacggc gcaggctgtc gtcgatagag cgcaaaattt gtgcacggga taatccttcc
    36421 ggtaaatcac caacgcgatg gaacactttc gtcgcaacga ccacgtcttc acgacgggcg
    36481 aaatcccgca gtgcgcgacc aacgatctct tcgctgctgc catcggaata gctattggcg
    36541 gtatcaaaga aatttatgcc gccttccagc gcgcgtttaa tgatgggacg gctgctctct
    36601 tccggcagtg tccatgcgtg attaccgcga tctggctcgc caaaggtcat acagccgagg
    36661 caaagtcggg aaacgcgaag gtcggttttt cctaaggggt tgtattgcat gctgccactc
    36721 ccgctatact cgtcatactt caagttgcat gtgctgcggc tgcattcgtt caccccagtc
    36781 acttacttat gtaagctcct ggggcttcac tcgtttgccg ccttcctgca actcgaatta
    36841 tttagagtct atgaataatt tcttaagcat agcaggagtg gagtagggat tatgccagcc
    36901 aggccttgat tttggcttcc ataccggcgg catcgaggcc gagttcggcg cgcatttctt
    36961 cctgagttcc ttgtggaata aagaagtcag gcaggccaat gttcagcacg ggtactggtt
    37021 tacgatgggc cattagcact tcgttcacgc cgctgcctgc gccgcccata atggcgtttt
    37081 cttctacggt gaccagcgct tcatggctgg cggccatttc cagaattaac gcttcatcaa
    37141 gcggtttcac aaaacgcata tcgaccagcg tagcgttcag cgattcagcg actttcgccg
    37201 cgtctggcat cagcgtacca aagttaagga tcgccagttt ctcgccacga cgcttcacaa
    37261 tgcctttgcc aattggcagt ttttccagcg gcgtcagttc cacgccaacc gcgttaccgc
    37321 gcgggtagcg caccgcggac gggccgtcgt tatagtgata gccggtatag agcatctggc
    37381 gacattcgtt ttcatcgctc ggggtcataa tgaccatttc cggtatgcag cgcaggtaag
    37441 agaggtcaaa agcgccctga tgggtttgac cgtcagcacc aacaatgccc gcgcggtcga
    37501 tggcgaacag gaccggaagc ttttgaatcg ccacgtcatg cagcacctga tcataggcgc
    37561 gttgcaggaa agtggagtaa atcgcgacaa tgggtttgta cccaccaatc gccagaccgg
    37621 cggcaaaggt caccgcgtgt tgctcggcga ttgccacgtc gaagtaacga tccgggaatt
    37681 tacgtgaaaa ctcgaccatg ccggaacctt cacgcatcgc cggagtaatc gccatcagct
    37741 tgttgtcttt cgctgccgtt tcgcacaacc agtcgccaaa gatttttgaa tagctcggca
    37801 aaccgccgct acttttcggc aaacaaccgc tggagggatc aaatttaggc acggcgtgga
    37861 aagtgatggg gtctttttct gccggttcat aaccacgacc ttttttggtc atgatatgca
    37921 ggaactgcgg gcctttcagg tcgcgcatgt tcttcagcgt ggtgataagc cccagcacat
    37981 cgtgaccgtc aaccgggccg atgtagttaa agcccagctc ttcaaacaac gtgccaggca
    38041 ctaccatgcc tttaatatgt tcttcggtac gtttgagcag ctctttaatt ggcggaacgc
    38101 cagagaaaac ttttttcccg ccttcgcgca gtgaagagta aagcttaccg gaaagcagct
    38161 gcgccagatg gttgttgagc gcgccgacat tttcggaaat cgacatttcg ttgtcgttga
    38221 ggaccaccag catatcagga cggatatcgc ccgcgtgatt catcgcttca aacgccatgc
    38281 ctgcggtaat cgcgccatcg ccgattacac agacggtacg gcgatttttg ccttctttct
    38341 cggcagcaac cgcaatacca attccggcac tgatggaggt tgatgaatgc ccgacgctta
    38401 atacgtcata ttcgctttcg ccgcgccacg ggaacgggtg caggccgcct ttctgacgga
    38461 tggtgccgat tttgtcgcgg cgtccggtca aaattttatg cggataagcc tgatgcccca
    38521 catcccaaat caattggtca aacggggtgt tgtagacata gtgcagcgcc acggtcagtt
    38581 cgaccgtgcc cagcccggag gcgaagtgcc cgctggaacg gctcacgctg tcgagtaaat
    38641 agcggcgcag ttcgtcgcag agtttcggta aactctcttt cggcaacagt cgtaactcct
    38701 gggtggagtc gaccagtgcc agggtcgggt atttggcaat atcaaaactc atcaggggcc
    38761 tattaatact tattgtttat ttattacgct ggatgatgta gtccgctagc gcttccagtg
    38821 ccgaggtatc gagtgattgt tcagccagtt gtttcagcga ctgacgggca tcgtcgatca
    38881 gatcccgggc tttcttccgg gcttgctcaa gacccagaag tgcagggtag gtacttttac
    38941 caagttgctg gtcggcaccc tggcgttttc ccaacgttgc agtatctcct accacatcca
    39001 ggatgtcatc ctgaacctgg aaggcaaggc cgatgctctc tgcgtacttg tcgagtactg
    39061 gcagagcacg acgcccttta tctccggcgc ttaatgcacc aaggcgaacg gcggcgcgaa
    39121 tcaatgcgcc ggttttatga cgatgaatac gctcaagcgc gtccagaggt acgtgtttgc
    39181 cttccgcgtc taaatctaat gcctgaccac cgcacattcc ggcaataccg ctggcgctcg
    39241 ccagttcaga aatcatcgaa attctgtcgc gatccgacac ttccggcata ttggcatcgc
    39301 ttagaatcga gaacgccagc gtttgtaaag cgtcgccagc gagaatcgcg tttgcttcgc
    39361 caaacttcac atggcaggtc ggcaaaccgc gacgcagatc gtcatcatcc atcgccggta
    39421 aatcatcatg aattaatgag taagcgtgga tacactctac ggcggcagcg ggtgcgtcca
    39481 gcgtgtttgt gctaacgcca aacatatgac cggtggcata aaccaggaag ggtcgcagac
    39541 gcttaccacc taataatgcg ccatactgca tggtttcgac cacgggagtg ttctgaaagg
    39601 gcagtggggc gataaaacgg ctcagcgcct ggttggcctg cttaacgcag gcttcgagtt
    39661 gctgcggaaa gtccattact cattgtccgg tgtaaaaggg gttagagagg cgtcttcatt
    39721 gtcagacagc agaatttgta cgcgctgttc ggcttgttgt aatttggcct gcccctgacg
    39781 tgccagctgc acgccgcgtt cgaactcgtt cagcgcctct tccagcggca ggtcgccact
    39841 ttccagacgg gttacaatct gttccagctc gctcagcgcc ttttcaaagc tggcgggagc
    39901 ctcatttttc ttcggcataa tgaatgtctg actctcaata tttttcgccc cgtcatggta
    39961 acggactcag ggcaaatagc aaataacgcg caatggtaag gtgatgtgcg cagcaaagcg
    40021 atgttagtgg tatacttccg cgcctggatg cagccgcagg tgtgggctgc tgtatttttc
    40081 cctatacaag tcgcttaagg cttgccaacg aaccattgcc gccatgaagt ttatcattaa
    40141 attgttcccg gaaatcacca tcaaaagcca atctgtgcgc ttgcgcttta taaaaatcct
    40201 taccgggaac attcgtaacg ttttaaagca ctatgatgag acgctcgctg tcgtccgcca
    40261 ctgggataac atcgaagttc gcgcaaaaga tgaaaaccag cgtctggcta ttcgcgacgc
    40321 cctgacccgt attccgggta tccaccatat tctcgaagtc gaagacgtgc cgtttaccga
    40381 catgcacgat atttttgaga aagcgttggt tcagtatcgc gatcagctgg aaggcaaaac
    40441 cttctgcgta cgcgtgaagc gccgtggcaa acatgatttt agctcgattg atgtggaacg
    40501 ttacgtcggc ggcggtttaa atcagcatat tgagtccgcg cgcgtgaagc tgaccaatcc
    40561 ggaagtgact gtccatctgg aagtggaaga cgatcgtctc ctgctgatta aaggccgcta
    40621 cgaaggtatt ggcggtttcc cgatcggcac ccaggaagat gtgctgtcgc tcatttccgg
    40681 tggtttcgac tccggtgttt ccagttatat gttgatgcgt cgcggctgcc gcgtgcatta
    40741 ctgtttcttt aaccttggcg gcgcggcgca tgaaattggc gtgcgtcagg tggcgcatta
    40801 tctgtggaac cgttttggca gctcccaccg cgtgcgtttt gtcgctatta atttcgaacc
    40861 ggtcgtcggg gaaattctcg agaaaatcga cgacggtcag atgggcgtta tcctcaaacg
    40921 tatgatggtg cgtgccgcgt ctaaagtggc tgaacgttac ggcgtacagg cgctggtcac
    40981 cggcgaagcg ctcggccagg tgtccagtca gacgctgacc aacctgcgcc tgattgataa
    41041 cgtctccgat acgctgatcc tgcgtccgct gatttcttac gacaaagagc acatcatcaa
    41101 cctggctcgc cagattggca ccgaagactt tgctcgcacg atgccggaat attgcggtgt
    41161 tatctccaaa agcccgacgg tgaaagcggt taagtcgaag attgaagcgg aagaagagaa
    41221 attcgacttc agcattctcg ataaagtggt tgaagaagcg aataacgttg atatccgcga
    41281 aatcgcccag cagaccgagc aggaagtggt ggaagtggaa accgtcaatg gcttcggccc
    41341 gaacgacgtg atcctcgata tccgttctat cgatgaacag gaagataagc cactgaaagt
    41401 cgaagggatt gatgtagttt ctctgccgtt ctataaactg agcaccaagt ttggcgatct
    41461 cgaccagaac agaacctggc tactgtggtg tgagcgcggg gtgatgagcc gtctgcaggc
    41521 gctctatctg cgcgagcagg gctttaacaa tgtgaaggta tatcgcccgt aatttgtggt
    41581 tgtaacgtcg catctggtca gatgcgacgc ttaccgcatc cgacactact cgtaataatt
    41641 ataaatccct gccgccatca ccagttgtga tgccacttca tgggctttct cgcgcccaac
    41701 cagcaggtcg ataattttca gaccaaagtc gatagctgtc cccggcccct ggctggtcag
    41761 caattttacc cgtgcatccc agacgacgcg cttgtcctgc cattgttcag cgggaatttt
    41821 gtctttcagc gtcgggaagc cggtcatatt accaatcggg aagatatcgt gcggcaccag
    41881 cacggtggct ggcgcggcgc aaatagccgc gacgatacgc ccggaacggt ggaactgttt
    41941 aacggtttca accagcaggg tactatcgcg aaaacactct gcgcctttaa tgccaccagg
    42001 cagtacgatc acgtcatatt cgccatcagc cacctcgacc agcggcgcat ccgccagcag
    42061 cttcacgccg cgcgagcagg taatggccag gttgccatcg ctggcgacgc tggcagtggt
    42121 gactttgata ccgccgcgaa ccagcagatc gatagtggtg acggcttcag tctcttcact
    42181 accaggggcg aggcaaacca gtgccgatgc gctcatattc actctccttt ctttttacca
    42241 tttcaaacag gcgggtgttt tccggtacgg caatcccatg cgcgcgggcg cggcgtaaga
    42301 gaaaaccatt gatatagtcg atttcggtgt ggcgcagcgc gcggatatcc tgcaacatcg
    42361 acgagatatt ttccgctgtg gcatcaatca cctgcatcac gtaatcacgc aaatcttctg
    42421 ctgaagtatg atgcccttcg cgctcgatca ctgccgcgac ttcttcgcat atctgcataa
    42481 tttcttgcgg atgatgacgt aattcaccat tcgggcaatt ccagatggcg gtcagtggat
    42541 taatcacaca gttgacggcc agcttgcgcc acagctcggc gcgaatattg ttatgccagg
    42601 caacgtcagg caacacggtt tgcaaaatat ccgccagata actgtaatcg ccatcctgtt
    42661 gccgtgccgg accaatatgc gtgattccgt ttgccacatg aataatgaca ttgccgtcgc
    42721 ggcgggcggc atgggtggtg gtgcccatca gtaatggctg ctgaatgttt tgcaactctt
    42781 cgatggtgcc catgccgttg tgaattaaca gtattggcgt ggttacaggc agtgtggacg
    42841 cgaggctttt gacggcatcg taaacctgcc atgctttcag cgtcaccagg agcagatcgc
    42901 tggtggcaag aaaatcggga tcgttggcgg tcagcgattc gttaaatatc gaaccatctg
    42961 tctcaaccag attcacgcta caataaggtt gcggtacgcg cagccagccc tgaacttcat
    43021 gaccctgttt gcaaagtgct gtaagccata attgccctaa ggcaccgcat cccaatacgg
    43081 taattttcat tgttcctcct cacccgcaac cactccgggt gttcaataag gctatccctt
    43141 aattgtgcat gctgttgcga ctatgcacaa ttaagggata cgtcctggtg caggactgtc
    43201 ggttatttaa ctttgcgggt attatgcttc gcatcaaaaa tgaagggaga ggaaaagatg
    43261 ccatctttcg atattgtctc tgaagttgat cttcaggaag ctcgtaacgc ggtcgataac
    43321 gcgagccgcg aagtggagtc ccgttttgac ttccgtaacg ttgaagcctc atttgagctg
    43381 aacgacgcca gcaaaaccat caaagtgttg agcgagtccg atttccaggt caatcagttg
    43441 ctggatattc tgcgtgccaa gctgctgaag cgcggcattg aaggcagttc gctggatgta
    43501 ccggagaata tcgttcatag cggtaaaacc tggtttgtgg aagcgaaact gaaacagggc
    43561 attgagagcg cgacccagaa gaaaatcgtc aagatgatca aagacagcaa actgaaagtg
    43621 caggcgcaaa ttcagggcga tgaaatccgc gtaacgggca aatctcgtga tgatttgcag
    43681 gctgtcatgg cgatggtacg tggtggcgat ctcggtcagc cgttccagtt caaaaacttc
    43741 cgcgattaat cgcgactatg tgcgttttgt tcatgtcaga tgcggcatga acgcctgatt
    43801 cggcctacaa aggtttgtaa ttcagaaact ttgtaggcct gataagcgta gcgcagcagg
    43861 caattcgccg cgttttttat gcctgacgaa ttgcctgttc tatctcaaag cgattcgtca
    43921 ctttgctgtc gattttcaca taagctgaat gttcttcttc tgcaatcaac acttctttga
    43981 cgccttcagt ttccagcaaa cgcactttta acgcttcgtt tgcggcaatg tccgccggga
    44041 tttcaatgcg caaactgctg acatacggcg gttctttcat ggtactggcg actgccagcc
    44101 acactgcggc cagcattgcg ccagcgagaa ataccccctg accgtcaaac atgccgtcaa
    44161 tccagccgcc cagcgaaccg ccaatcgcca cgccaagaaa ctggctggtg gagtagacac
    44221 ccatcgccgt acctttgtaa cctgctggcg actctttact gataagtgag ggcagaaggg
    44281 cttccatcaa attaaacgcc acaaagaaaa gctgcacgcc gaccaccagt tgccagaact
    44341 gcgtttgtgc gttccacaac acaatttccg caaccacgat caacccgacg cagaagacaa
    44401 agacttgctt cattttgcgc ttaacttcag cgtagataat gaaaggcacg accgagccaa
    44461 aggcgattaa catcgtcgcc agatagacct tccagtgttc agccgccggg aaccccgcat
    44521 cagctagctg tccgggcagg gcaacaaacg tagacatcag aagcatgtgc agacacataa
    44581 tgccaaagtt gagttttagc agccgcggtt ccgccagcac tttactgaaa ctgcctttca
    44641 ccattccgga ctcacgatta agtacgtgag tgctactgtt gggcacaacc caaatggtca
    44701 acgcaatgcc ggttgttgcc agaatagcga tcatccagaa cagcgcgtgc agcccaagtt
    44761 tgtgagtgat gatcgggcca agcaccatcg caatggcaaa ggtaatgcca aagctcacgc
    44821 cgataaacgc catcgctttg gtgcggtttt gttcgcgcgt gagctcggaa agcagcgcca
    44881 taacggcggc ggcaattgct ccagagcctt gtagcgcccg gcccagaata attccccaga
    44941 tggagtcgga aagcgcagcg ataacgctac cggcggcaaa caccgccagc ccaccgacaa
    45001 ttaatggttt gcgaccaatg cggtcggaaa gcaggccaaa cggaatctga aaaacggcct
    45061 gagtcagacc ataaatacca atggcaatac cgattaatgc ttcgctggca ccttgcagag
    45121 ccatgccgta cgtggtcaga accggcagaa ccataaacat gcccagcatg cgcaacgaga
    45181 atacggtccc taaaccccag gtcgcgcgcc tctcacctgg cgtcatttta taatcgttca
    45241 ttaccacccc ttaaaaaaat gcccacatag tgtagtgcgg agcgcatgtt gggtaaacag
    45301 agtgttaatg agaaaatatt acagaggaga gtaatttgtg tgtcaggtat gtttttttgc
    45361 attcataaaa tgaatgctct atatgttgaa aaaatgaatt ttaaaactaa atagttttac
    45421 atcaaaaaag taataatatc atttcttgat tgctatgtgc attttttatc ttacgaatat
    45481 gctagtttta tctcactaaa taaacgattt ggtgacatgg atgataatgt ttcaggctaa
    45541 atgtgtgctt attacttgga cctttatggc actgtattta ttcattaaat ggatatttgg
    45601 tggttaatta ttatggaaat atatgacagt gaggacataa aggtatacat gttaactaaa
    45661 agaatattat tttctgtaat gctgatagtt tcaccaagcg tggtggccag cgagaaagcg
    45721 catgaattgt atgacagcat atacggtggt aaacctgctc ccgatgttat aaatacactg
    45781 cataaaatgg ctgaatcggg agatatcgac gcgcagagcc tgttaggctg ggaatattat
    45841 cagcctcgtt atgataccaa acctgatgtt caggaagcga ttaaatggtt cgagttagcg
    45901 gctaaacaag gtgacagaga agctccgtta gcgctggggg gtatctacta cgacggtgag
    45961 caggtgcggg tggattatgc caaagcgtat gcactattta atcaggccgc gcagcacggt
    46021 gtgaatttag caaggtccag gttgggcatt atgtacgcca atggtcagta tgttgaggta
    46081 gattgtaaga aagcgaagga atatcttgat aaaggtgtcc acatttatgg tggcccagaa
    46141 gactttctgg ctacttgtcg aaaagacatg attgacagaa aaacggttga cgatacgtta
    46201 cccgtgatta cggttacccg ttctgggatg cgagataatt ttttagataa aggtttttcc
    46261 tgcatggata gcctcttcgc caccaccaat aaattaggcg aagtggccaa tcttcgcgtg
    46321 acattcagca ttcgtcgccc atccggtaag gagattaacc aaacggttgg cttcgcgcct
    46381 ttcggtttaa accggctgaa tattagcttt actgattacc tatttggttc atttaccagt
    46441 aattcgtctc ttattcttta taaaccggaa tttgagcgaa aatcttgtgc aactgtaagg
    46501 accacaatcg ttgccgcaac ggcaaccatt aacggcaagg atgtggagtt gctgaaagcg
    46561 ggggcaattg aacaaaagtg gtaacgctgc tcagcccccg gctgaggcaa aacttgccct
    46621 ggcgggtggc gataataatc taagccgcga gaatatgctg aatcgccaaa aacataggta
    46681 cagcaccaaa agcatttggt gtcgctgaaa gctgaacaaa gccgagacga gcataaaaat
    46741 taatggcggc agggtcagca tcaagataaa cccctttaat tggtaatgcc tggtgaatta
    46801 tttttacatg ttcaaaaaaa tcacatagta ggtcctgacc aaagccccgc ttttgatact
    46861 tccgtgctac ccccaacatg actaatctga caacaccaat ttctgaaggt tgtgaaccct
    46921 gaaggacgcc agaaacgcgt tgtttttcca gcgaatatgc cgtaaaagta cagatgccga
    46981 tcagttcacc actttgtctg tcaataagtg ctttagccgc acagtcgcta ttacgcacac
    47041 ttttctttag cgatgcgcgt acaaatttat cgataacagg gttaccgcaa tcaaactgtt
    47101 tttgcccagg ataagtaata tcagactgat aattacagga gaggactatg ggcagagtaa
    47161 tctcttcatg cttgtcgacc atcgctgttt ttccttttca ttaacgcctt gagggcgagc
    47221 gtcggttgag caggttcagc gatgagttgg ttcatcagtt cccagctttg attcgaaagc
    47281 tcacggcgac gttggttatc aagcacactt tcggcgcgtt ccatagcagc gctcaaaata
    47341 aaagcgctta aatctacccc aacggcttgc gcagcttcac gaagtttgtt tttggcgtcg
    47401 ggactagttt tgagttcgac ccgcgcattt ttcagcgttt tatcgacatc gggtagagtg
    47461 gctgtttttc caggcattat gacctctttg attaactgaa tgaaatcgta gggagtcgat
    47521 ctcatacata atacggctat caaccgtatg attaatttat gttcatctgc tcactctgtc
    47581 aagacatacg gttattagcc gtatctctga tctcagacaa taaaaaaggt gccgtagcac
    47641 ctttttaata gagaggtttt gttaccacac agcagccagc agcgtatgcg agtccggtac
    47701 cataaaatca acggacatca tcaccgagag ggcagtgatg gcgatgatag agaagccgaa
    47761 cagcttgcgc gcccagattc tgtcatcagc aactttataa ccgcgcagag ccatacctaa
    47821 ccaccagacg ctaaccgccg cggcgaccac cagatattta tatccagcgt aaccgccaag
    47881 agagagcatc agcgtggcaa cggcaaaggc gatgatatac agcgtgatgt gattcttcgc
    47941 caccgaaatg ccttttacca ctggcaatac cggaatgttt gccgcctggt aatccttaaa
    48001 gcggaaaatg gcgatggcat aggagtgagg catctgccac aggctgaaga tagccagcag
    48061 gatcgctgcg ccgctatcga actcgccggt tactgcacag tagccaatca ccggcggcgc
    48121 agcgccggag agcgaaccaa tcaatgtgcc gtagactgag tggcgtttca tgtacaggct
    48181 atagacgccg acataaacca caaagcccat cacccccagc cagcaggcca gcggattcgc
    48241 gccaaaccac agcagcataa agccagcaat acccagcaag gtggcgtaca ccagcgagac
    48301 agcaggagag atcaggcctt taaccagcac ccgattcttc gtcctttcca tctttctgtc
    48361 gatatccctg tcgatgtagt tgttaaacac acaacccgac gccacaacca gtgacacccc
    48421 aaccagcgtg tagataaaca ggggataatc aatgctgccc tttgaggcca gcaggaatcc
    48481 cccaatcacc gagatcaggt tgccaaagat gatgcctggt ttcgttactt gcaggtattg
    48541 cttaaacatc ataaccgccg ctcttagtgc atcatcatgt tgtagttgag gttccacata
    48601 atccagatgg agcctacaac caggatagcg atgattagca cggtgaagac aaacgccgtc
    48661 atgttccagc cttcatctga tttggtattc atgtgcagga agcacaccag atgcaccaga
    48721 acctgtacca ctgccattgc caggattgtt cccagaatta cggccggaga ggcagcccct
    48781 gtcatcacca tccagaacgg aatcaccgtc agaatgatcg acaggataaa gcctgtcatg
    48841 taggttttta cgctgccatg ggacgcgccg ctgtgatcgg tagaatgact cattacatcg
    48901 cccccatcag ataaacaaca gtgaacacac agatccaaac cacatccagg aagtgccaga
    48961 acaggctcag gcacatgatg cgggtacggt tagtgctggt caggccgcga cgagcgattt
    49021 gtaccatcag caccgccatc cagataagac cggaagtgac gtgcagaccg tgcgtgccga
    49081 ccagcgcaaa gaacgctgac aggaagccgc tgcgatccgg acccatgccg ttaacaatca
    49141 ggtgatggaa ttcgtagatt tccatcccga taaatccggc accaaacaac caggtcaacg
    49201 ccagccagga gataacctgg cttttgttgt ttttgtacat ggcgatagcc gccatgccgt
    49261 aggtgatgga gctgaacaac agcaagaaag tttcaaccag aacgaacggc agttcgaaaa
    49321 tgtccttacc tgtcgggccg cctgcggtgc cgttcaccag aacggcatag gtagcaaaca
    49381 agattgagaa cagaatgcag tcgctcatca ggtagatcca aaatccgaag attttggttc
    49441 cgcctgcatc gtggtgcccg tgttcgtgcg cgtgggcagt cgcgtgcgtc aaagtatcag
    49501 ttgccatttt tcagccctgc cttagtaatc tcatcgaaat gctggttttc cagtttttcg
    49561 atttctgcca ccggcacgta gtaatccacg tcctcgtcga agcttttcac gatccaggtg
    49621 atgatcatgc ctgcgaagcc aacaatcgcc agccaccaga tatgccagat catggcgaaa
    49681 ccgaagatgg tggagaaagc tgcaatgacg atacctgcac cgctgttttt cggcatatga
    49741 atttcttcat agtggtcagg ctttttatac gcttcgcctt tctctttcat ttcccagaat
    49801 gcatcacgct cgtgaacgtg cggcacaacg gcaaagttat agaacggagg cggggaagag
    49861 gttgcccact ccagcgtacg gccaccccac gggtcgccag tcaggtcacg gttctggtcg
    49921 cggtcgcgaa tagaaacgta catctgaata acgaggcaga gaatacccag cgcaatcagt
    49981 actgcaccgc tagctgcaat catcagcatg gtgtggaact gcgggtcaat ctgctggctc
    50041 aaacgacggg tcatgcccat gaagcccagc gcatacagcg gcataaaggc aacgaagaag
    50101 ccgatgatcc agaaccagaa cgcgcgttta ccccaggttt cattcagttt gaaaccgaag
    50161 gctttaggcc accagtaggt catccctgcg aagcagccga agaccacgcc gccgatgatc
    50221 acgttatgga agtgcgcaat caggaacagg ctgttatgca gaacgaagtc cgcgcccggt
    50281 actgccagca gcacgccagt catcccgccc accgagaaag tgacgataaa accgatggtc
    50341 cacagcatcg cagaatggaa cacgatgcgg ccctgataca tggtgaacag ccagttgaag
    50401 atcttcaccc cggtcgggat ggcgataatc attgtggtga taccaaagaa ggcgtttacg
    50461 ttcgcgcccg cacccatcgt aaagaagtgg tgcagccaaa cgatgaacga cagcacggtg
    50521 atacagacgg ttgcccatac cagcgaggta taaccaaaca gacgtttacg cgagaaggtt
    50581 gccgcaattt cggagaacac accgaaaaca ggcaggatca ggatgtaaac ttccgggtgg
    50641 ccccaggccc aaatcaggtt gatgtacatc atcatgttgc cacccatatc gttggtaaag
    50701 aaatgggtgc ccagatagcg atccagggtc aacaacgcga cggtaaccgt cagaattggg
    50761 aaggaagcaa taatcagtac gttcgcgcac agtgatgccc aggtaaatac tggcatcttg
    50821 aacatggtca tgcccggtgc gcgcatcttc agaatggtaa cgaagaagtt gataccggta
    50881 agcgtcgtac ctataccgga tagctggaga ctccatatcc agtaatcgac accgactccc
    50941 ggactgtact ctattcccga tagcggtgga taggccagcc agccggtctg cgcaaattcg
    51001 cccacgccga gagaaacgtt aaccagaatc acaccaacaa cagtaaacca gaagcttaag
    51061 ttgttgagga acgggaacgc aacgtcacgc gcgccgatct gcagcggaac caccaggttc
    51121 atcagaccga taacgaaagg catcgccacg aagaagatca taatcacgcc gtgcgcggtg
    51181 aagatctgat cgtagtggtg aggtggcagg aagcccgctt cgcccgccga ggcaagagcc
    51241 tgctggctac gcatcataat ggcgtcagca aaaccacgca gcaacatcac aatcgccacg
    51301 atgatataca tgataccgag gcgtttatgg tcgacggagg tcagccactc tttccacagg
    51361 taggtccact taccgaagta agtgatcagg ccaacgagcg ccagacctcc caaaataatg
    51421 ccagcgatcg taaccatgac gataggttca tggaacggga ctgcatcaag tgataatttt
    51481 ccgaacatct ttattcttcc tcaacccctt taatgggcgg attccgcgtg gctcatgtcc
    51541 atgccttcca taccttcgtg tgcactgtgc tcaccttctg gctgggtcat gtccatgctc
    51601 ttaccgtgag ccataaactt gttaatcaca tcggcaaaca agtctggttt cacgttggag
    51661 aaatattcca cctggttgta ttcgctaggc gcggccagtt tttcgaacgc agccatgtca
    51721 gacatggtgt tcggcgactg tttcgctttt gcgacccatt ggtcgaattc ggcgcgatcc
    51781 ggtgttgcaa tagctttgaa cttcatgcct gagaagcccg gcccgctgta gctggcggag
    51841 ataccgtcat aagtaccggg ttcgttggcg atcagatgca ggcgagtctg cataccggcc
    51901 atggcataaa tctggctacc cagacgcgga atgaagaagg agttcatcac ggagttggag
    51961 gtcactttga agtacaccgg agtgttcgcc gggaaagcga tttcattcac ggtagcaatg
    52021 ccctgttccg ggtagatgaa gaaccatttc cagtccatgg aaaccacttc gatggtaatg
    52081 ggcttctcgt cgtgtgccag cggcttgcta ggctcaagag cgtgagtggt tttccaggtc
    52141 agtaccgcaa ggaagatgat gattaagata ggtaccgtcc agaccacagc ttccacttta
    52201 ttggagtgtg accagttcgg gctgtactta gcatctttat tgctcgcacg atacttccag
    52261 gcgaaaccaa cagccatcaa gattgcggga ataacgacaa tcaacatcag gccaaatgcc
    52321 gtcagtatca gtgaacgttg ctccagacca atctgtcctt tgggatctaa cagcgcagaa
    52381 ttacagccac tgagcaatac agtgcctgca aataatgaca accatcccaa acttttattg
    52441 tatttcctga gtctcattta acgacctcaa ttccacggga tctggtggcg tttaaagtgt
    52501 gtgggcattt tacgggaagg ttacattact gtaaacatga ttaaattcct gttacttgga
    52561 tttggctgtc aggtcacata tgttgcaaaa catatcaagt tgattttaag aagctgttgc
    52621 aaaagggcgt tataacgaaa ggggagtgca gaaggagttt ggacgggcaa ttggtataac
    52681 caatctggaa taaaacaatt gtttaacaaa taattatcat tatggtgaca attgggtggg
    52741 ggaaaataaa gactcattag ctgaatcgtg taagaaaaaa tagaattcat aaattgcgtc
    52801 aataatttcc aaacgctaag ccgcacaaaa gaacaaatat tattaagcga tactgatgtt
    52861 tatttagccg taataattac ggcgagtgat tactacagct aaataatatt tacagattac
    52921 gtcagatgcg tttttcgtag cgccagataa tccagcaaac caccaagcac gacgccagaa
    52981 agggcgacta aaaccccgac ttccagcaga gcaggcagga aagagaagtg cgtcaaatcc
    53041 agcgcgtcca tcgtcaacag cagtaaccac acggccaaca ggctgacgcc cgccgccagt
    53101 gtccacatgg caaaggcata acctgccgga tattcggtac gggagataaa gttgtcattt
    53161 actcctgtat attcaagcgt ctggcggcaa accagcagca aaataagccc cggtacggca
    53221 gcggcgacgg agaatagata gaatgtcgac cagccgtgtg cttcaacaaa ccaacccgcc
    53281 acggggccga cataaactcg tcctacagcg gaaagcgctg agagcagggc aaattgagtt
    53341 gcggaaaatg acttattaca cagcgtcatt aacagcgcga caaaggctga tgtgcccatc
    53401 ccgccacaga ggttttcgaa aaagacggct gcgcccatgc tgtagagatg cttatcagta
    53461 atcgacagca gccagtaacc agcgttagac gcaccttgta aaatgccgaa aatcagcagc
    53521 gcccggaaca gcgacaggcg ctgcatcaaa atcccaccgt acaatgcgcc cacaatggtc
    53581 gccagtaagc caagcgtttt gttaaccacg cctacttcac ccgcatcaaa cccgacgccg
    53641 cgaatcaaaa acgtggttgt caggctcatg gcgaatgcgt cgcccagctt atacagcacg
    53701 ataagaagca aaataagcca ggcattattg cgaccaaaga aatctcgcag aggtgcaaca
    53761 accgcttgtt ccagcgtttt tggcacagga atggtgtcgg tcggttctgg tgcaagcaac
    53821 gtcgcgataa tacaagggat caacagcgcc gccatcaacc agtacatgcc ctgccagccc
    53881 agccatttat ctgccagcca cagggctaag ccgccggaaa ccagcatccc taaacggtaa
    53941 cccagcacgc tgattgccgc gcccgcacca cgttcttctg ccgggagcac atcggttttc
    54001 cacgcatcga agacaatatc ctgggaggcc gagcaaaaag cgatcaccac cgccagtgcc
    54061 gccatccagc ggagttgggt gccaggttcg agaaaaccca tcgccgcaat ggcgactaat
    54121 aacaggattt gcgtggcgag cagccaaccg cgccgccgcc caaaaaatgg gggcgtgtag
    54181 cggtccatca gcggtgacca gaggaattta aaaacgtaag cctggcctac cagagagaag
    54241 aaaccaatgg ttttgagatc aatattctcg accgtcatcc aggcctgtaa ggtgccggat
    54301 gtcagggcga gcggtagccc ggaagcaaaa cccaggatca gcaatatggc tgaacgcggc
    54361 tgttgaaaaa tacgtaaata ttgactggac atgggcgtct acaggcccgg cttgcaccgg
    54421 gccagagggc agaaattaac gcgcgttctg cttgatgaat tcgtgaatgc tggtgtcctg
    54481 agacatatca gcgatggtat cggtcagcac actgttaacc gcatcggcga tatttttgtt
    54541 ggaggcctgg aacgcacctt caacgttgta gctggcacga tagtttttgg tcattttgtt
    54601 gccattctgc gcggtagcga tgatggcgat atccgctttg gtagcgatgt tgtagcgcac
    54661 gttgccctgg gacacgtcag catacagttg gctaacgatg atttgcagat taaccgggcc
    54721 attcggacca accatgtaac cacgcgcggt catctgtttt tccagcactt cttgcagcag
    54781 gaaacgcaga tcgcgggagg cggtcagggt aacgatttga ttatcgcggg tgacttttgc
    54841 cagcgcctga tcggtacgct gatcggcacc attaatgctt acggtgacgc ccatcaggct
    54901 tggatcctgc tgtggcagtg taatcgtcgg ggaaacttca atagttgttg gcggttttgc
    54961 gcatcctgca agcataaaca gagcaactaa cgggaagagg atttttttga acatgttcgg
    55021 gctctcagag actcttaagc gtgtttggta aaaattcccg ccatcataac attgccaacg
    55081 gcgaggggaa gtgggtaagg catgtaaatt catcatgttg acgaaataat cacccctggt
    55141 aaaagaaacg ctgatgcgag gcctgtgttt caatctttaa atcagtaaac ttcatacgct
    55201 tgacggaaaa accaggacga aacctaaata tttgttgtta agctgcaatg gaaacggtaa
    55261 aagcggctag tatttaaagg gatggatgac atctcagcgt tgtcggagga gatatttcat
    55321 gatgatacgt gagcggatag aagaaaaatt aagggcggcg ttccaacccg tattcctcga
    55381 agtagtggat gaaagctatc gtcacaatgt cccagccggc tctgaaagcc attttaaagt
    55441 tgtgctggtc agcgatcgtt ttacgggtga acgttttctg aatcgtcatc gaatgattta
    55501 cagtacttta gcggaggaac tctctactac cgttcatgcg ctggctctgc atacttacac
    55561 tattaaggag tgggaagggt tgcaggacac cgtctttgcc tctcctccct gtcgtggagc
    55621 aggaagcatc gcgtaaaaac gcatttgcaa ctgtcggcgc ttttccagta tgttgctaaa
    55681 gattttatga aaaacggcct gcgggccgtt ttgttttgtc tggattttgc gctttttgcc
    55741 cagcattcag acgaaaattg cccgggaatt gtgaaaaaat acgcgacagc gcgcaataac
    55801 cgttctcgac tcataaaagt gatgccgcta taatgccgcg tcctatttga atgctttcgg
    55861 gatgattctg gtaacaggga atgtgattga ttataagaac atcccggttc cgcgaagcca
    55921 acaacctgtg cttgcggggt aagagttgac cgagcactgt gattttttga ggtaacaaga
    55981 tgcaagtttc agttgaaacc actcaaggcc ttggccgccg tgtaacgatt actatcgctg
    56041 ctgacagcat cgagaccgct gttaaaagcg agctggtcaa cgttgcgaaa aaagtacgta
    56101 ttgacggctt ccgcaaaggc aaagtgccaa tgaatatcgt tgctcagcgt tatggcgcgt
    56161 ctgtacgcca ggacgttctg ggtgacctga tgagccgtaa cttcattgac gccatcatta
    56221 aagaaaaaat caatccggct ggcgcaccga cttatgttcc gggcgaatac aagctgggtg
    56281 aagacttcac ttactctgta gagtttgaag tttatccgga agttgaactg cagggtctgg
    56341 aagcgatcga agttgaaaaa ccgatcgttg aagtgaccga cgctgacgtt gacggcatgc
    56401 tggatactct gcgtaaacag caggcgacct ggaaagaaaa agacggcgct gttgaagcag
    56461 aagaccgcgt aaccatcgac ttcaccggtt ctgtagacgg cgaagagttc gaaggcggta
    56521 aagcgtctga tttcgtactg gcgatgggcc agggtcgtat gatcccgggc tttgaagacg
    56581 gtatcaaagg ccacaaagct ggcgaagagt tcaccatcga cgtgaccttc ccggaagaat
    56641 accacgcaga aaacctgaaa ggtaaagcag cgaaattcgc tatcaacctg aagaaagttg
    56701 aagagcgtga actgccggaa ctgactgcag aattcatcaa acgtttcggc gttgaagatg
    56761 gttccgtaga aggtctgcgc gctgaagtgc gtaaaaacat ggagcgcgag ctgaagagcg
    56821 ccatccgtaa ccgcgttaag tctcaggcga tcgaaggtct ggtaaaagct aacgacatcg
    56881 acgtaccggc tgcgctgatc gacagcgaaa tcgacgttct gcgtcgccag gctgcacagc
    56941 gtttcggtgg caacgaaaaa caagctctgg aactgccgcg cgaactgttc gaagaacagg
    57001 ctaaacgccg cgtagttgtt ggcctgctgc tgggcgaagt tatccgcacc aacgagctga
    57061 aagctgacga agagcgcgtg aaaggcctga tcgaagagat ggcttctgcg tacgaagatc
    57121 cgaaagaagt tatcgagttc tacagcaaaa acaaagaact gatggacaac atgcgcaatg
    57181 ttgctctgga agaacaggct gttgaagctg tactggcgaa agcgaaagtg actgaaaaag
    57241 aaaccacttt caacgagctg atgaaccagc aggcgtaatt tacgcagcat aacgcgctaa
    57301 attcgcacaa aggcccgtca ccgccaggtg gtgggctttt ttttgtcatg aattttgcat
    57361 ggaaccgtgc gaaaagcctc tttcggtgtt agcgtaacaa caaaagattg ttatgcttga
    57421 aatatggtga tgccgtaccc ataacacagg gactagctga taatccgtcc ataaggttac
    57481 aatcggtaca gcaggttttt tcaattttat ccaggagacg gaaatgtcat acagcggcga
    57541 acgagataac tttgcacccc atatggcgct ggtgccgatg gtcattgaac agacctcacg
    57601 cggtgagcgc tcttttgata tctattctcg tctacttaag gaacgcgtca tttttctgac
    57661 tggccaggtt gaagaccaca tggctaacct gattgtggcg cagatgctgt tcctggaagc
    57721 ggaaaaccca gaaaaagata tctatctgta cattaactcc ccaggcgggg tgatcactgc
    57781 cgggatgtct atctatgaca ccatgcagtt tatcaagcct gatgtcagca ccatctgtat
    57841 gggccaggcg gcctcgatgg gcgctttctt gttgaccgca ggggcaaaag gtaaacgttt
    57901 ttgcctgccg aattcgcgcg tgatgattca ccaaccgttg ggcggctacc agggccaggc
    57961 gaccgatatc gaaattcatg cccgtgaaat tctgaaagtt aaagggcgca tgaatgaact
    58021 tatggcgctt catacgggtc aatcattaga acagattgaa cgtgataccg agcgcgatcg
    58081 cttcctttcc gcccctgaag cggtggaata cggtctggtc gattcgattc tgacccatcg
    58141 taattgatgc cagaggcgca actgtgccgc tatacttatc cagggcggca caacgctgta
    58201 agcggcttgc gcctgagaat ggcatttgcg tcgtcgtgtg cggcacaaag aacaaagaag
    58261 aggttttgac ccatgacaga taaacgcaaa gatggctcag gcaaattgct gtattgctct
    58321 ttttgcggca aaagccagca tgaagtgcgc aagctgattg ccggtccatc cgtgtatatc
    58381 tgcgacgaat gtgttgattt atgtaacgac atcattcgcg aagagattaa agaagttgca
    58441 ccgcatcgtg aacgcagtgc gctaccgacg ccgcatgaaa ttcgcaacca cctggacgat
    58501 tacgttatcg gccaggaaca ggcgaaaaaa gtgctggcgg tcgcggtata caaccattac
    58561 aaacgtctgc gcaacggcga taccagcaat ggtgtcgagt tgggcaaaag taacattctg
    58621 ctgatcggtc cgaccggttc cggtaaaacg ctgctggctg aaacgctggc gcgcctgctg
    58681 gacgtcccgt tcaccatggc cgacgcaacc acgctgaccg aagccggtta tgtgggcgaa
    58741 gacgttgaaa acatcattca gaagctgttg cagaagtgcg attacgacgt acagaaagcg
    58801 cagcgcggga ttgtctacat cgatgagatc gacaagattt ctcgtaagtc agacaacccg
    58861 tccattaccc gagacgtttc cggtgaaggc gtacagcagg cactgttgaa actgatcgaa
    58921 ggtacggtag ctgctgttcc accgcaaggt gggcgtaaac atccgcagca ggaattcttg
    58981 caggttgata cctctaagat cctgtttatt tgtggcggtg cgtttgccgg tctggataaa
    59041 gtgatttccc accgtgtaga aaccggctcc ggcattggtt ttggcgcgac ggtaaaagcg
    59101 aagtccgaca aagcaagcga aggcgagctg ctggcgcagg ttgaaccgga agatctgatc
    59161 aagtttggtc ttatccctga gtttattggt cgtctgccgg ttgtcgcaac gttgaatgaa
    59221 ctgagcgaag aagctctgat tcagatcctc aaagagccga aaaacgccct gaccaagcag
    59281 tatcaggcgc tgtttaatct ggaaggcgtg gatctggaat tccgtgacga ggcgctggat
    59341 gctatcgcta agaaagcgat ggcgcgtaaa accggtgccc gtggcctgcg ttccatcgta
    59401 gaagccgcac tgctcgatac catgtacgat ctgccgtcca tggaagatgt cgaaaaagtg
    59461 gttatcgacg agtcggtaat tgatggtcaa agcaaaccgt tgctgattta tggtaagccg
    59521 gaagcgcaac aggcatctgg tgaataatta accattccca tacaattagt taaccaaaaa
    59581 ggggggattt tatctcccct ttaatttttc ctctattctc ggcgttgaat gtgggggaaa
    59641 catccccata tactgacgta catgttaata gatggcgtga agcacagtcg tgtcatctga
    59701 ttacctggcg gaaattaaac taagagagag ctctatgaat cctgagcgtt ctgaacgcat
    59761 tgaaatcccc gtattgccgc tgcgcgatgt ggtggtttat ccgcacatgg tcatcccctt
    59821 atttgtcggg cgggaaaaat ctatccgttg tctggaagcg gcgatggacc atgataaaaa
    59881 aattatgctg gtcgcgcaga aagaagcttc aacggatgag ccgggtgtaa acgatctttt
    59941 caccgtcggg accgtggcct ctatattgca gatgctgaaa ctgcctgacg gcaccgtcaa
    60001 agtgctggtc gaggggttac agcgcgcgcg tatttctgcg ctctctgaca atggcgaaca
    60061 cttttctgcg aaggcggagt atctggagtc gccgaccatt gatgagcggg aacaggaagt
    60121 gctggtgcgt actgcaatca gccagttcga aggctacatc aagctgaaca aaaaaatccc
    60181 accagaagtg ctgacgtcgc tgaatagcat cgacgatccg gcgcgtctgg cggataccat
    60241 tgctgcacat atgccgctga aactggctga caaacagtcc gttctggaga tgtccgacgt
    60301 taacgaacgt ctggaatatc tgatggcaat gatggaatcg gaaatcgatc tgctgcaggt
    60361 tgagaaacgc attcgcaacc gcgttaaaaa gcagatggag aaatcccagc gtgagtacta
    60421 tctgaacgag caaatgaaag ctattcagaa agaactcggt gaaatggacg acgcgccgga
    60481 cgaaaacgaa gccctgaagc gcaaaatcga cgcggcgaag atgccgaaag aggcaaaaga
    60541 gaaagcggaa gcagagttgc agaagctgaa aatgatgtct ccgatgtcgg cagaagcgac
    60601 cgtagtgcgt ggttatatcg actggatggt acaggtaccg tggaatgcgc gcagcaaggt
    60661 caaaaaagac ctgcgtcagg cgcaggaaat ccttgatacc gaccattatg gtctggagcg
    60721 cgtgaaagat cgcatccttg agtaccttgc ggttcaaagc cgtgtcaaca aaatcaaggg
    60781 accgatcctc tgcctggtag ggccgccggg ggtaggtaaa acctctcttg gtcagtccat
    60841 tgccaaagcc accgggcgta aatatgtccg tatggcgctg ggcggcgtgc gtgatgaagc
    60901 ggaaatccgt ggtcaccgcc gtacttacat cggttctatg ccgggtaaac tgatccagaa
    60961 aatggcgaaa gtgggcgtga aaaacccgct gttcctgctc gatgagatcg acaaaatgtc
    61021 ttctgacatg cgaggcgatc cggcctctgc actgcttgaa gtgctggatc cagagcagaa
    61081 cgtagcgttc agcgaccact acctggaagt ggattacgat ctcagcgacg tgatgtttgt
    61141 cgcgacgtcg aactccatga acattccggc accgctgctg gatcgtatgg aagtgattcg
    61201 cctctccggt tataccgaag atgaaaaact gaacatcgcc aaacgtcacc tgctgccgaa
    61261 gcagattgaa cgtaatgcac tgaaaaaagg tgagctgacc gtcgacgata gcgccattat
    61321 cggcattatt cgttactaca cccgtgaggc gggcgtgcgt ggtctggagc gtgaaatctc
    61381 caaactgtgc cgcaaagcgg ttaagcagtt actgctcgat aagtcattaa aacatatcga
    61441 aattaacggc gataacctgc atgactacct cggtgttcag cgtttcgact atggtcgcgc
    61501 tgataacgaa aaccgtgtcg gtcaggtaac cggtctggcg tggacggaag tgggcggtga
    61561 cttgctgacc attgaaaccg catgtgttcc gggtaaaggc aaactgacct ataccggttc
    61621 gctcggcgaa gtgatgcagg agtctattca ggcggcgtta acggtggttc gtgcgcgtgc
    61681 ggaaaaactg gggatcaacc ctgattttta cgaaaaacgt gacatccacg tccacgtacc
    61741 ggaaggtgcg acgccgaaag atggtccgag tgccggtatt gctatgtgca ccgcgctggt
    61801 ttcttgcctg accggtaacc cggttcgtgc cgatgtggca atgaccggtg agatcactct
    61861 gcgtggtcag gtactgccga tcggtggttt gaaagaaaaa ctcctggcag cgcatcgcgg
    61921 cgggattaaa acagtgctaa ttccgttcga aaataaacgc gatctggaag agattcctga
    61981 caacgtaatt gccgatctgg acattcatcc tgtgaagcgc attgaggaag ttctgactct
    62041 ggcgctgcaa aatgaaccgt ctggcatgca ggttgtgact gcaaaatagt gacctcgcgc
    62101 aaaatgcact aataaaaaca gggctggcag gctaattcgg gcttgccagc ctttttttgt
    62161 ctcgctaagt tagatggcgg atcgggcttg cccttattaa ggggtgttgt aaggggatgg
    62221 ctggcctgat ataactgctg cgcgttcgta ccttgaagga ttcaagtgcg atataaatta
    62281 taaagaggaa gagaagagtg aataaatctc aattgatcga caagattgct gcaggggctg
    62341 atatctctaa agctgcggct ggccgtgcgt tagatgctat tattgcttcc gtaactgaat
    62401 ctctgaaaga aggggatgat gtagcactgg taggttttgg tacttttgcc gttaaagagc
    62461 gtgctgcccg tactggccgc aacccgcaga ccggtaaaga gatcaccatc gctgctgcta
    62521 aagtaccgag cttccgtgca ggtaaagcac tgaaagacgc ggtaaactaa gcgttgtccc
    62581 cagtggggat gtgacgaagt tcaagggcgc atctactgat gtgccttttt tatttgtatt
    62641 cggtgacttt ctgtgtcttg tgggctgaca attgcccccg tttcttgtca caataggcct
    62701 ttgcgcgcat cgatacgttg cgtgaggtac acagtcatct acagcggagt gttgttacac
    62761 catgatggac agcttacgca cggctgcaaa cagtctcgtg ctcaagatta ttttcggtat
    62821 cattatcgtg tcgttcatat tgaccggcgt gagtggttac ctgattggcg gaggcaataa
    62881 ctacgccgca aaagtgaatg accaggaaat cagccgtggg cagttcgaga acgcctttaa
    62941 cagcgagcgt aatcgcatgc agcaacagct gggcgatcaa tactccgagc tggcagcgaa
    63001 cgaaggctat atgaaaaccc tgcgtcaaca ggtgctgaat cgtctgatcg acgaggcgct
    63061 gttggatcag tacgctcgtg agctgaaact gggtatcagt gatgagcagg ttaaacaggc
    63121 gattttcgcg accccagcct tccaggttga cggcaaattt gataacagcc tctataacgg
    63181 tatcctcaac cagatgggga tgaccgccga tcagtacgcc caggcgctgc gtaaccagct
    63241 cactacccaa cagctgatta acggtgttgc cggtaccgat tttatgctga aaggtgaaac
    63301 cgacgagctg gcggcactgg tcgcgcaaca acgcgtggtg cgtgaggcga ctatcgatgt
    63361 taacgcgctg gcggcgaagc agcctgtgac cgaacaggag attgccagct actacgaaca
    63421 aaacaaaaac aatttcatga cgccggaaca attccgcgtg agttacatca agctggatgc
    63481 cgcaacgatg cagcaaccgg ttagcgatgc ggatatccag agctactacg atcagcatca
    63541 ggatcaattc acccagccgc agcgtacccg ctacagcatc atccagacca aaactgaaga
    63601 tgaagcgaaa gtggtacttg atgagctgaa taaaggcggt gattttgctg cattagccaa
    63661 agaaaaatct gccgatatta tctctgcccg taacggcggc gatatgggtt ggttagaaga
    63721 tgccactatc cccgacgaac tgaaaaatgc tggtctgaaa gaaaaagggc aactgtctgg
    63781 tgtcatcaaa tcttcggtcg gtttcctgat tgtacgtctg gacgacattc agccagcgaa
    63841 agtgaaatcg ttagacgaag tacgtgacga cattgcggcg aaagtgaaac acgaaaaagc
    63901 cctcgatgcg tactatgcgc tgcagcagaa agtgagcgat gcggcaagca acgacacgga
    63961 gtctctggcc ggtgcagagc aagctgccgg cgttaaagcc actcagacgg gttggtttag
    64021 caaagataac ctgccggaag agttgaactt caagccggtt gccgacgcta tctttaacgg
    64081 cggtctggta ggtgaaaacg gcgcgccggg catcaactct gacatcatca ccgtagacgg
    64141 cgaccgcgca ttcgtgctgc gcatcagcga gcacaaaccg gaagcggtga aaccgttggc
    64201 agatgttcag gaacaagtta aggcactggt tcagcacaac aaagctgaac aacaggcgaa
    64261 agtggatgct gagaaactgc tggttgattt gaaagccggc aaaggtgcgg aagctatgca
    64321 ggctgccggt ctgaaatttg gcgagccgaa aatcttaagc cgttccggtc gtgacccgat
    64381 tagccaggcg gcgtttacac tgccactgcc agcgaaagac aaaccgagct acggtatggc
    64441 gaccgatatg caaggtaatg tggttctgct ggcgctggat gaagtgaaac aaggttcaat
    64501 gccggaagat cagaaaaaag cgatggtgca gggtatcacc cagaacaacg cacaaatcgt
    64561 ctttgaagct ctgatgagta acctgcgtaa agaggcgaaa atcaaaattg gcgatgcgct
    64621 ggaacagcaa taatcctgaa gccgcctcgc aaaaaaatgc gttgcagctg taacaagaaa
    64681 aggtcgcttt cgcggccttt tccatttctg aacattgcca tttgtttact gttttcactc
    64741 ccgttaaggt gattccactg ttaacaaaca aggagaaaac agtatgaaac acggaattaa
    64801 agcactgctc attaccctgt ccctggcctg tgccggaatg tctcatagcg cgctggcggc
    64861 agcttctgtg gcgaaaccga cggcggtaga aaccaaagcg gaagctcctg cagcacaaag
    64921 taaagcagca gtaccggcga aagccagtga cgaagaaggc acccgggtca gcattaataa
    64981 tgccagcgcg gaagagctag cccgcgcgat gaatggcgtt ggcctgaaga aagcgcaggc
    65041 gattgtcagt tatcgcgaag agtacggtcc gtttaaaacg gtggaggatc taaagcaggt
    65101 gccggggatg ggcaattcgc tggtggaacg taatctggcg gtattaaccc tgtaattaat
    65161 ttgcatagtg gcaatttttt gccagactga agaggtcata ccagttatga cctctgtact
    65221 tataacaaca acgtaaggtt attgcgctat gcaaacacaa atcaaagttc gtggatatca
    65281 tctcgacgtt taccagcacg tcaacaacgc ccgctacctt gaatttctcg aagaagcccg
    65341 ctgggatggg ttggaaaata gcgacagttt tcagtggatg acggcccata acatcgcctt
    65401 cgtcgtggtc aatatcaata ttaactatcg tcgcccagcg gtattaagcg acctgttaac
    65461 tattaccagt cagttgcagc aattaaacgg taaaagcggc atcttaagcc aggtcattac
    65521 actggagccg gaagggcagg tggtagcgga tgcgcttatt acgtttgttt gtattgatct
    65581 taaaacgcag aaagcattag ctctggaagg ggaattgcgc gaaaagctgg agcagatggt
    65641 taagtaaacg ttttgtggtg ccggatgctc aagccgcatc cggcgacacc cggaataatt
    65701 acctcaaccc ggttttctgc ttcatcgctg ccatcaccgt cggtttatcg gccagataat
    65761 gattcaaacc gttggcgcgt aaattacatg ccgcacaatg accgcaaccg tcgcctttaa
    65821 agccgttata gcaggtcaac gtttcgttac ggactaaatc cagtttgccg taataatctg
    65881 ccagcgccca ggtttccgct ttatcaatcc acatcagcgg cgtttcaaaa cgaatatctt
    65941 tcgccatgcc caaactgacg gcatggttta gtgctttcac aaactcatcg cggcaatccg
    66001 ggtagccgga gaaatccgtt tcacagacgc cggtaattac ggcttctgct tttacctgat
    66061 acgcatatat tgccgccagc gtcaggaaca aaatattacg cccagggaca aacgtattcg
    66121 ggatgccatc ggcttcaggt tcataatcag gcaccggaat gctgtcacgt gtcaggctgc
    66181 tgaccgccag ctcgttgagc agcgttacat caagaacctt atgcgcgcgt gctcccagtt
    66241 tcagcgccag ttcgcgtgcc acgtcgattt ctgcgcgatg tcgctgaccg taatcgaacg
    66301 tcacgcaatg gacttcatca tattgttgta atgcctgcac cagacaggtg gtggaatcct
    66361 ggcctccact gaacacaacg acagcacgtt tcatagataa tcctgcttga acaataaaag
    66421 cgttatggta acgcctgcga ttaacccgga ccagctattc attgcgacgg cgcgggaagc
    66481 caggcttcgg taaattcaaa ccagccgcgc ggtgtcagtc gcacaccgtt cacgccggga
    66541 ggggcactaa tgcgatagtg atagttgaac agcggcgtca gcgtcgcgtc tgccattaac
    66601 tggctaaaaa ccgctttcag ggcattaaat cggttttcct catcaggcat tacttgcacc
    66661 gcgtccagcg tcgattgcag atgtgcatat gctggagcgt cgaaaacatg tggccatagt
    66721 ggatcgcaac gtagccattg ctccagagta tattccggtg cttcgccaat taatctgtcg
    66781 cccatcatga ggtctgcgtg tgccagtagg gtcgtgtcgt cccagttttt tgcgttatga
    66841 aaaataattg tgagttcaca gccttccgct gccagtgtcg cctgtaggcg ttctgccatg
    66901 gtatgaagtt ctatcggtag gtgataaacc agcgtcaagg ttttcggtag tttgacttca
    66961 tccggtactt gccagtacgg aatagtccag cctggcagta atgcatgact ggcggtgatc
    67021 aggttttctc cgacttctaa cgtttgtaat aaaccggatt gatgaataat ggagatcact
    67081 tttcgcgcct gccagaggga gagtcgggga cttttgcgca acgttaaata gcaaaaacct
    67141 aaactgatgc cactactgac ctggctgacc cgttgcagct cctccggttt gccgatggtg
    67201 atttgcacgg gatgccgaca actgtttccc aaatcttttt cgaaaagcgg cggagttatc
    67261 cagtactcaa ccgctttaag cagcggatga cgcaaatggt aataatcatg gctctccagg
    67321 cgcaccagct ccgctgtgaa ttgtgttaag cgaaaaggac cagtgccgat cagtgggaat
    67381 tgcggatgcg ccagatggct gcaatagctc gccagccggt gcgcaagcca gtaatcgggg
    67441 cgatgtaaaa agaaggtcag acactgcgga tgggtgactt caatatgctt cacgctaata
    67501 aataattgat ccagtgctgg cagttgtaac agcatcaata atcgctggtg taagtgtgag
    67561 gcttttactg catcgccgtt atgccagtgt agggttgaac gaagataaaa gtcccagcgt
    67621 aacccgtcag tagaggtttc ccagtgatgc gctaaatcgc caatcgggcg ctgagtatta
    67681 ttatcgaagc gggtcaggcc ggaaaatatc tgcccggcga gatgctgctc ggcacggccg
    67741 ggcaaaaagc ctggttgtag cggttcgagc gggcgatagt agggaatacg caacgtgggt
    67801 gtatcgtttt gccattgtcc gcccataaac ggctgtaaca gagtgcgcag ctcacctggg
    67861 gccagttgcg ccagctccag cacatcttgc tgctttccgg tttccagtgc ctgttccatc
    67921 atcgcattgc gtagcgattc cggcgtgacc agaaagcgta attgtccgcg ctttccgcgt
    67981 cctgactgcg cctgccactc cagccatccc gcctcctgtg cctgacgcaa cagcgtacga
    68041 acatggcgtt cgctgcaaaa acagcgttcg gccagttcgc tgacggtgac ggtttgcggc
    68101 tttccggctg aaggttgcca cagacgttga tactggttaa gacggttgag caatcgcata
    68161 taaacccgga acaatattat ttaactattc actattactt ccgtatatat caggtgatac
    68221 tcaatcacca ttaaccgtgt cacagagtgg agaaagaaat ggctcgtctg gcagcatttg
    68281 atatggatgg cactttattg atgcctgacc atcatttagg tgagaaaacc ctctctactt
    68341 tggcgcgact gcgtgaacgc gacattaccc tcacttttgc cacggggcgt catgcgctgg
    68401 agatgcagca tattctcggg gcgctatcgc tggatgcata tttgattacc ggcaacggaa
    68461 cgcgcgtgca ttctctggaa ggtgaacttt tacatcgtga tgatttacct gcggatgtcg
    68521 cggagctggt gctgtatcag caatgggata cccgagccag catgcatatc ttcaatgacg
    68581 acggttggtt taccgggaaa gagatccctg cgttgttgca ggcatttgtc tatagcggtt
    68641 ttcgttatca gataatcgat gtcaaaaaaa tgccactcgg cagagtcacc aagatctgct
    68701 tctgtggcgg tcacgacgat cttacacgct tgcagatcca gctatacgaa gcattaggcg
    68761 agcgtgcaca tttgtgtttt tccgccacgg attgcctcga agtgctgccg gtgggctgca
    68821 ataaaggcgc tgcattgacg gtgctgaccc aacatttagg tttatcgttg cgcgattgca
    68881 tggcctttgg tgatgcgatg aacgatcgcg aaatgttagg cagcgtcggt agcgggttta
    68941 ttatgggcaa tgcgatgccg caactgcgcg cggagctccc gcatttaccg gtgattggac
    69001 attgccgaaa tcaggctgtc tctcactatt tgacgcactg gctggactat ccacatctac
    69061 cttattcccc cgaataacga gatcccttcc agcaccgggc aattgcccgg ttttttttgc
    69121 gttgaatttg tcattttgtg ccgtggtgtt taaaccgcac agaataaatt gtcgtgattt
    69181 cacctttaaa atagaattaa aagagaaaaa aattctctgt ggaagggcta tgttagataa
    69241 aattgaccgt aagctgctgg ccttactgca gcaggattgc accctctctt tgcaggcact
    69301 ggctgaagcc gttaatctga caaccacccc ttgctggaag cgtctgaaac ggctggagga
    69361 cgacggtatc cttatcggca aagtcgccct gctggatccg gaaaaaatag gcctcggcct
    69421 gaccgctttt gtgctgataa aaacgcaaca tcacagcagc gaatggtatt gccgctttgt
    69481 cacggtggtt accgaaatgc cagaagtgct ggggttctgg cgcatggctg gtgaatacga
    69541 ttatctgatg cgcgtccagg ttgccgacat gaaacgctac gacgagtttt ataagcgtct
    69601 ggtaaacagc gtgccggggc tgtcggacgt cacttccagc ttcgcgatgg aacagattaa
    69661 atacaccact tctttaccca tcgaataaat atccagaatc aggtcaggac acaacgcgtg
    69721 cgattatttg ctcaattaag ctggtatttc cgtcgggaat ggcgtcgcta tctcggggct
    69781 gtcgccttgc ttgtcattat cgcgatgctg caactggttc cgccaaaagt ggttggtatt
    69841 gttgtcgatg gcgtgacaga acaacacttt actaccgggc tgatcctgat gtggatcgcc
    69901 accatggtgc tgattgccgt tgtggtttat ctcctgcgtt atgtctggcg cgtattgctg
    69961 tttggcgcgt cttaccaact ggctgttgaa ctgcgtgaag attattaccg tcagctaagc
    70021 cggcagcacc cggagtttta cctgcgtcat cgcacgggtg atctcatggc tcgtgcgacc
    70081 aatgacgtcg atcgcgtcgt gtttgccgcc ggagaagggg tgctgacgct ggtggattca
    70141 ctggtaatgg gctgcgctgt gttgattatg atgtctacgc aaattagctg gcagttgacc
    70201 ttatttgccc tgttaccgat gccagtgatg gcgatcatga ttaagcgcaa cggcgatgcc
    70261 ttgcatgaac gctttaagct ggcacaggcg gcgttttcca gtcttaatga ccgcacccag
    70321 gaaagcctca ccagtatccg catgatcaaa gcctttggtc tggaagatcg ccagtcggcg
    70381 ttatttgccg cggatgccga agataccggc aaaaaaaaca tgcgggtggc gcgtattgat
    70441 gctcgtttcg acccgacaat ctatatcgcg attggtatgg cgaacttgct ggcgattggc
    70501 ggcggtagtt ggatggtggt gcagggcagt ttaacgctgg gccagctcac cagttttatg
    70561 atgtatttag gtctgatgat ttggccaatg ctggcgctgg catggatgtt taacattgtg
    70621 gaacgtggta gtgctgcgta cagccgtatt cgtgcgatgc tggcggaagc gccggtggtg
    70681 aacgatggta gcgaacccgt gccggaaggg cgtggcgaac ttgatgtaaa tattcaccag
    70741 ttcacgtatc cgcagactga ccatcgtgcg ctggaaaacg tcaatttcgc cctgaaaccc
    70801 ggtcagatgc tgggtatctg cgggccgact ggttccggca aaagtaccct gttgtcgctc
    70861 attcagcgtc atttcgacgt cagcgagggg gatattcgct ttcatgatat tcctctgacg
    70921 aagttacaac tcgatagctg gcgtagccgc ctggcggtag ttagccagac accgttcctt
    70981 ttttccgaca ccgtagcgag caacatcgcg ttgggttgcc cgaatgccac ccagcaagag
    71041 attgagcatg tcgcgcggtt agccagcgta catgacgata ttttgcgttt accgcaaggt
    71101 tacgatacag aggtgggcga gcgcggcgtg atgctttccg gcgggcaaaa acagcgtatc
    71161 tctattgctc gtgcgttatt agtcaacgcg gaaatcctca tccttgatga tgcgctttcg
    71221 gcggtggacg gacgcacaga gcaccagatc ctgcataacc tgcgtcagtg gggacagggt
    71281 agaacggtaa tcatcagtgc ccatcgcctt tcggcactga cggaagccag tgaaattatt
    71341 gtgatgcagc acggacatat cgcccagcgt ggcaatcatg atgagctggc gcaacaaagc
    71401 ggctggtatc gcgatatgta tcgctatcaa caactggagg cggcgctcga cgacgctccg
    71461 gaaattcgcg aggaggccgt cgatgcgtag ttttagccaa ctgtggccga ctctcaagcg
    71521 tctgttagcg tacggttcgc cgtggcgtaa accgctgggg attgcggtcc tgatgatgtg
    71581 ggttgcggca gcggcagaag tcagtgggcc gctgcttatc agctatttta tcgacaatat
    71641 ggtagcgaaa aataacctgc cgttgaaaat ggttgcaggg ctggctgcgg cgtatgttgg
    71701 gctacaactg tttgccgccg ggctacatta cgcgcagtcg ctgctgttta atcgggcggc
    71761 agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat gccgcattgc gccagccatt
    71821 aagcgagttt gatacccaac ccgtcgggca ggtgatttcc cgcgtcacta atgacactga
    71881 agtgatccgc gatctctacg ttaccgtagt ggcaactgtc ctgcgtagcg ccgcactggt
    71941 gggcgcgatg ctggtggcga tgttcagcct cgactggcga atggcgctgg tggcgataat
    72001 gattttcccg gtggtgctgg tggtaatggt gatataccag cgttacagca cgccgattgt
    72061 ccgtcgtgtg cgcgcctatc tagcggatat caacgacggc tttaacgaaa tcatcaatgg
    72121 catgagcgtt atccagcagt tccgtcagca ggcgcgattt ggcgaacgta tgggggaggc
    72181 cagtcgttca cactatatgg ccaggatgca aaccctgcgc ctcgacggtt ttctgctgcg
    72241 tccgctgctg agtctgtttt catcgctcat tctttgtggc ttgttgatgc tgtttggctt
    72301 ctccgctagc ggcaccattg aagtgggcgt gctgtatgcg tttatcagct atcttgggcg
    72361 acttaacgaa ccattaatcg aactgaccac gcaacaggcg atgctgcaac aggctgttgt
    72421 tgctggtgag cgcgtgtttg aactgatgga cggaccgcgc cagcaatatg gcaatgatga
    72481 tcgcccgtta cagagtggca ccatcgaagt cgataacgtg tcatttgctt atcgcgatga
    72541 caatctggtg ctaaagaaca ttaatctctc tgtgccttcg cgtaactttg tggcactggt
    72601 cgggcatacc ggcagtggca aaagcaccct cgccagttta ttgatgggct attacccgct
    72661 aacggaaggt gagattcgcc ttgatggtcg tccattaagt tcgctaagtc acagcgcgct
    72721 gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg ctggcggata ccttcctcgc
    72781 caacgtgacg ctggggcgtg atatctccga agaacgcgtc tggcaggcgc tggaaaccgt
    72841 gcaactggcg gagctggcgc gcagcatgag tgatggtatt tatacgccgc tgggcgagca
    72901 ggggaataat ctctccgtcg ggcaaaagca actgctggca ctggcacgcg tgttggtgga
    72961 gacgccgcaa atcctgatcc ttgatgaggc aaccgccagc attgactccg gtactgaaca
    73021 ggcgattcaa catgctctgg cggctgtgcg tgaacatacc acgctggtgg tgattgctca
    73081 ccgcttatcg accattgttg atgccgacac cattctggtg cttcatcgtg ggcaagccgt
    73141 ggagcagggc actcaccagc aactgctggc ggcccaggga cgctactggc agatgtatca
    73201 actgcaactt gcgggcgaag agctggcagc cagcgtgcgt gaagaagaat cattgagcgc
    73261 ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa cgcactgtgc actgtcatag
    73321 tgcgttttca ttttcaatct tcttaacttc ctgctctctt tctcgttttt catttctggc
    73381 acaccgcttg caataccttc ttcgtgtagc agaaccatta ccgaattctg accggagggg
    73441 atctatgaag ctggtgaccg tgataatcaa accattcaag ctggaagacg ttcgtgaagc
    73501 gttatcttcc attggtattc agggcctgac cgtcaccgaa gtgaaaggtt tcgggcgtca
    73561 gaaagggcat gccgagctgt accggggggc ggaatacagc gtcaacttcc tgccaaaagt
    73621 aaaaattgat gtggcgattg ctgatgacca actcgatgaa gtgatcgata tcgtcagtaa
    73681 ggcggcttac accggaaaaa ttggcgacgg caaaatcttc gtcgctgaat tgcaacgcgt
    73741 cattcgtatt cgtaccggcg aagccgacga agcggcgctg taatctctgg cacacagcaa
    73801 caggaacgaa aaatgaagat agcgacgata aaaactgggc ttgcttcact ggcgatgctg
    73861 ccgggactgg taatggctgc acctgcggtg gccgataaag ccgacaatgc gtttatgatg
    73921 atttgtactg cgctggtgct gtttatgact attccgggga ttgccctgtt ttacggtggg
    73981 ttgattcgcg gcaaaaacgt gctgtcgatg ctgacgcagg tgacggtgac gtttgcactg
    74041 gtctgtattc tctggggggt ttacggttac tcgctggcgt ttggtgaggg caacaacttc
    74101 ttcggcaaca ttaactggtt gatgctaaaa aacatcgaac tgacggcggt gatgggcagc
    74161 atttatcagt atatccacgt ggcgtttcag ggctcgtttg cctgcattac cgtcggcttg
    74221 atcgttgggg cgctggcgga acgaatccgc ttctcagctg tgttgatttt cgtggtggta
    74281 tggctgacgc tctcttacat tccgattgcg catatggtgt ggggcggtgg tttgctggct
    74341 tctcacggtg cgctggattt cgcgggtggc acggtggtgc atattaacgc cgcaatcgcc
    74401 ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct tcggcaaaga ggcgtttaaa
    74461 ccgcacaacc tgccaatggt cttcaccggt actgccattc tctatattgg ttggtttggc
    74521 tttaacgccg ggtcagcggg cacggcgaat gaaatcgcgg cactggcatt tgtgaatact
    74581 gtggtcgcaa cggcggcggc aattcttggc tggatcttcg gtgaatgggc gctgcgtggt
    74641 aagccttctc tgctgggagc gtgttctggc gcgattgccg gtctggtcgg cgtgacgcca
    74701 gcgtgtggct acattggggt tggcggcgcg ttgattatcg gcgtggtagc tggtctggcg
    74761 ggcttgtggg gcgttactat gctcaaacgc ttgctgcggg tggatgatcc ctgcgatgtc
    74821 ttcggtgtgc acggcgtttg tggcattgtc ggctgtatca tgactgggat ttttgccgcc
    74881 agttcgctgg gtggcgtggg cttcgctgaa ggtgtgacga tgggccatca gttgctggta
    74941 cagctggaaa gcatcgccat tacgatcgtc tggtccggtg tggtggcatt tattggctac
    75001 aaactggcgg atctgacggt tggtctgcgt gtaccggaag agcaggagcg agaagggctg
    75061 gatgtcaaca gtcacggcga gaatgcctat aacgcgtaac aagcactgca aaaaatagcc
    75121 ggacggtttt acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg
    75181 gtcgaggcaa ccagcacgcc gtcctgggta taaaactcac cgcgcacaaa gccgcgtgcg
    75241 ctggacgccg aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga
    75301 tggaaccaca tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgatg
    75361 ccgtgcggct gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac
    75421 tgatgaacgc gcaggtcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgt
    75481 ggttctgcga cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga
    75541 cgatcgcaga tgaatttatc tttaagcact ggcggcagca ggtgcgccag cgattgggcg
    75601 atttgcgttt ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt
    75661 tcgaaacccg cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt
    75721 tgaatagcag caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca
    75781 taaataatcg gcttcttact atcgccaggg cgaagaaagt agctgtgaaa cgaatgtacc
    75841 agccgctctt cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg
    75901 ccaaacacct ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt
    75961 ttttccagat ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt
    76021 aacaaagctg ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ctactggtcc
    76081 gatgggtgca atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca
    76141 cacttgttga cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg
    76201 tgcattgata ataaggagaa atgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt
    76261 gcgattgcgt tggcggcttg cgcagataaa agcgcggata ttcagacgcc agccccggct
    76321 gcaaatacat ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt
    76381 accgtctgga tccggcagaa agtcgcactg ccgcctgatg ctgtgctgac cgtgacactt
    76441 tctgacgcgt cgttagccga tgcaccgtca aaagtgctgg cgcagaaagc ggtgcgtacc
    76501 gaaggtaaac agtcaccatt cagctttgtt ctgccattta atccggcaga tgttcagccg
    76561 aacgcgcgta ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc
    76621 gataccgttc agccggtgat caacaagggc ggaactaaag ccgacctgac attggtgccg
    76681 gtgcagcaaa ccgccgtgcc ggttcaggcc agcggtggtg caacgactac cgtaccttcg
    76741 acttcaccaa ctcaggtgaa tccgtcttcg gcagttccag ctcctacgca atattaagcg
    76801 acgttaaccc tctccgcccg gagagggtta gtagttccag cgataacgct gcaagtcgat
    76861 ttgcccgctt cccgatacca tcacaccttc tgccagtaat gcctgtcgct gacgctgtaa
    76921 atccggtccg gttagcgaaa ttgtgccgtg gcgattaacc acccggtgcc agggtaaggt
    76981 gctgccttca gggagacgct ttaacacacc gcccacctgg cgcgcggcgc ggggcgatcc
    77041 cgccagtttc gccacatcac cgtaagtggt gacatagcct tcgggaatag cggcgacgat
    77101 ttgccagacg cgttggggaa atgaatcttc tttttccatc ttttcttcct gaggtaattt
    77161 ttcagcataa tctggaaaaa cgcccgagtg aagtcgcatt gcgcaagaaa ccagcatctg
    77221 gcacgcgatg ggttgcaatt agccggggca gcagtgataa tgcgcctgcg cgttggttct
    77281 caacgctctc aatgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca
    77341 ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc tggcagggcc
    77401 cccacccatt tctgcctccc accgtttcgt caaaaaaccc caacatggct aaactttaac
    77461 cacaactgac gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt atgtctgatg
    77521 aagtatgttg atggttttgt ggttgccgtt cctgccgata aaaaggatgc ctatcgggaa
    77581 atggccgcta aggctgcgcc attgtttaaa gagtttggcg cgcttcgtat tgtcgaatgc
    77641 tgggccagcg atgtaccgga tggcaaagtg accgattttc gtatggcggt gaaagcggaa
    77701 gagaatgaag aggttgtctt tagctggatt gaataccctt caaaagaggt ccgcgacgct
    77761 gctaatcaaa agatgatgtc ggacccacgg atgaaagagt tcggcgagtc catgccgttt
    77821 gacggcaagc gaatgatcta tggcggattc gagtcaatca tcgacgaata ggtagcgtga
    77881 cgggctgtgc ggcgtgccgt cagcccaaaa attcacaaat gttgctcggc ccagcgtaag
    77941 aaatcttctt tcggtaatgc cttgctgtag agccagccct gaccgtagtg cacgccatgc
    78001 tggcgtagcc actcttcttg tttactggtt tcgattccct ccgctaccat tttcagtttc
    78061 agtgtttttg ccatttcgat gatatgcggc gtgacatttt tatattccag cgcatcaacg
    78121 aaagatttat cgatcttcag aatgtcgaca tccagatcct gtaaataact taaacttgaa
    78181 tatcccgtac caaaatcatc aagataaatt tcatggcccg cctcccggta gcgagaaatt
    78241 atcggggcgc tggttttcgg atcggcaaac tcgcgttcag tgagttcaag cgcgatctgt
    78301 ctgggattaa cctgatagtg attgatcatt tcacgcagca attgcgggat tttttccgag
    78361 gtgagcacgg tggattcaag attgatcgaa atatgctgct gtggatgctg acgcagccag
    78421 tcgcccatat cttcaaagac gcttcttata atcagtagcg tcaatggctc agaaaggccc
    78481 gtttgctgtg ccagcggaat aaaactatct ggtgacaacc aactaccgtc tgtctgcggc
    78541 cagcgcgcca gtgcctcagc accgacaatt ttgccattgg ctaaggagac aatcggctga
    78601 tagtgcacgc aaatatcacg attttcgata gcatcctgca gccgatgatg cggtgactgg
    78661 atacggcgca aaatacgcag cacaaacatc gctgccagca ggccaatcac caacccgagc
    78721 ggtaaccaaa taaagacttg ccgatgccag cctttctgca acattttcgt tgaagcccag
    78781 gtgatgatcg aaatattcat ctccgggaag ggcaggatat cgtaaatgat tccattattt
    78841 tcgatatgct caccgggtgt tttttgtagc ctggtaataa ttccctgagc aatttcatcg
    78901 ctgctggtta tgacaacgtt atgggcattg ccaataatgg cggcatcaat ttgccatgag
    78961 ctatagggaa tgacatcaat aaaggaagcg gggtcgatca tgacgacata atgtgccgtt
    79021 cccatggcga ccatgtaacg gataatgcct aaatcgttat gcgatgttaa ccagacacga
    79081 taaccatctt tcgaaatttt acctggctcg gggaaggtat cgggcggact ttcatgctcc
    79141 agagacgaac actggggaac gttgttatcg atataaacca cttcctgaat atagcgataa
    79201 ctgtaagata ccagacgcat ttccatgaga tgggcttcgc tacaggcagc gccttgccat
    79261 ctttccagct cttgcagcgc atctttccct tgtgtcgcca ccttattggc ccgaatagcg
    79321 acgcgggagg aataggtatc cagctcttca ataaacgatg tttctacctg ctgatgggcc
    79381 agccagatgc ttaagccgac aggcagcaat actgaaagaa tcagtactcc cgaaataagg
    79441 ccgaccagat gtcgtgttct caccagcata tccttattaa cacagtgtag ggaaagtata
    79501 tctgattgtg gaaaaaggct ggcaggagat aaggaggtag agctggtatt tcatgaagtg
    79561 atgggtggaa acgatcattg tcatttgacc aggcgcagag ctttacgcct ggtatgtaac
    79621 cctaacttaa gatgttgatt cggcgcgagc gagggtaaaa atatcgtaaa aagacagttc
    79681 acctttacgg acgatcattt tttgcagttg ttccttatga acatccgcga cacgcggaga
    79741 gttcaagatg gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt
    79801 atagcaaacg cggaaatcca gcacgtcgat atcttcatag cggtagcgtg ggtagacatc
    79861 gaaaaagaag aaaccattaa gcaggataaa taatacaacc agtagccaga cagagcctga
    79921 cagcgtttcg gactgcaaca tcaccgccag ggcggcaaaa aaagcaacgt acataccgat
    79981 aaacagcccc ggatgtttac ggataaaact gatactaaag cggggtttgt tgtcgcgttt
    80041 ttcgcgggta ttcagagact caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt
    80101 ttgccttcgt ggtatctgca actttccagg gaacacccta ttttaacgtg ggaatagtag
    80161 aggggaagta acagatctac cacaattttg cataacagtt gcgaaaaacc gattacaatt
    80221 ttttaattat tctggctgga ttaccgccca cgacaacgtt gtccgggaca tcttttgtga
    80281 caactgcacc tgaggctacc acgacgttat caccaatggt cacaccaggg ttaatgaccg
    80341 cgcgtccgcc aatccagacg ttattaccga tggtgacggg tttccccagt tcagcaccgc
    80401 tattacgtgc tacagggtcg atgggatgtg ttgccgtgta gatatgaacg cctggtgcca
    80461 acatacagtt atcaccgatg cgaatagggc agacatcaag catcacgcaa tcgaagttgg
    80521 cgaaaaaatt attaccgaga aaaatgttat agccatagtc acagcgaaac gttggctcaa
    80581 tataagcctc tgtcacctga ccgaatagat cagcgagaat ttgctggcgt aatgtgtgct
    80641 cttccgccag ggaatgattg tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag
    80701 ataacgtctc atctgccgag cgatacaact caccagcaat catcttttct ttttctgtgc
    80761 tcattactga cctccggttg ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa
    80821 gagaacgttc ccattacatc tgtgtgataa agatcacata gggtatgtct tcatggtgaa
    80881 aaagtataaa attcttaata aacagccggt tatagctccg aaagcgaatt atttattagc
    80941 gaataaattt ccatactgag gaagggatct tgtcgtatag tttattcatg gtcaattcgg
    81001 cgaggcggtg atctgcggct gagtaaaata ccgccagttc attatctgat aattcgtatt
    81061 tatttttctc gataacacgc tccagcgtgt caattgtctg gcaacgacgt aaacgcatta
    81121 aataatcggt tttcgttaaa ggtttttcgg acatacttct acccatggtt gtaataattc
    81181 taacaagata aactcgcagg attctctttc gtcgcattga caaaacaacg gaatagtcga
    81241 ttacctgact tccgccattt ctgaagatcc tgcatattaa taccataact gctgaacaac
    81301 ataaaggtgt catccagata ttcgtcgatc tgctcaatga gcttattgtc ttcattatac
    81361 ttaattttgt aattaagtgc gaaggtcgca atatgctcaa tcagttcatt caactggagg
    81421 ttgatcgccg aggttgggtc gttaacccag ccatgattgc tttcttcaag gtttgcaaga
    81481 cagtcatgat acagggtttc acagagaaac ttaagctgtg cgatatcatg tcttttgggt
    81541 gagtattcat ccataacgcg tccccttctt agtggttgaa ctaacggaca cctttcggga
    81601 tggaaaaaac ttactgacct ggacttgccc tctcgtttgt tagcttaact ataagccact
    81661 ctttgcaggt tttcatcgca ttattacgaa aaattacaat taggaaggaa tcgggacagg
    81721 actcactttt tcatactagc actaacccgc agcaggtaaa agcagtttgc tcgaactgtt
    81781 cataacattt caggattatc gccacatgaa attggtgaca ctaataccag gattgctctg
    81841 aatatgacgt aataaccgag gaatgaataa agaattaccg caaa
//
LOCUS       DAZUFB010000009        72826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZUFB010000009 DAZUFB010000000
VERSION     DAZUFB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.009"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            250..834
                     /gene="yadN"
                     /locus_tag="ACHY3M_000728"
     CDS             250..834
                     /gene="yadN"
                     /locus_tag="ACHY3M_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4315700.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            939..1679
                     /gene="yadV"
                     /locus_tag="ACHY3M_000729"
     CDS             939..1679
                     /gene="yadV"
                     /locus_tag="ACHY3M_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS4315701.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1714..4314
                     /gene="htrE"
                     /locus_tag="ACHY3M_000730"
     CDS             1714..4314
                     /gene="htrE"
                     /locus_tag="ACHY3M_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFS4315702.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESNPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4331..4900
                     /gene="yadM"
                     /locus_tag="ACHY3M_000731"
     CDS             4331..4900
                     /gene="yadM"
                     /locus_tag="ACHY3M_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4315703.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4915..5517
                     /gene="yadL"
                     /locus_tag="ACHY3M_000732"
     CDS             4915..5517
                     /gene="yadL"
                     /locus_tag="ACHY3M_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFS4315704.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5544..6140
                     /locus_tag="ACHY3M_000733"
     CDS             5544..6140
                     /locus_tag="ACHY3M_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFS4315705.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6193..7466
                     /locus_tag="ACHY3M_000734"
                     /pseudo
     CDS             6193..7466
                     /locus_tag="ACHY3M_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7579..8373
                     /gene="panB"
                     /locus_tag="ACHY3M_000735"
     CDS             7579..8373
                     /gene="panB"
                     /locus_tag="ACHY3M_000735"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS4315706.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8385..9236
                     /gene="panC"
                     /locus_tag="ACHY3M_000736"
     CDS             8385..9236
                     /gene="panC"
                     /locus_tag="ACHY3M_000736"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS4315707.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPSLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9310..10212)
                     /gene="rpnC"
                     /locus_tag="ACHY3M_000737"
     CDS             complement(9310..10212)
                     /gene="rpnC"
                     /locus_tag="ACHY3M_000737"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFS4315708.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10486..10866
                     /gene="panD"
                     /locus_tag="ACHY3M_000738"
     CDS             10486..10866
                     /gene="panD"
                     /locus_tag="ACHY3M_000738"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS4315709.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10870..12099)
                     /gene="yadE"
                     /locus_tag="ACHY3M_000739"
     CDS             complement(10870..12099)
                     /gene="yadE"
                     /locus_tag="ACHY3M_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4315710.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12163..12603)
                     /gene="yadI"
                     /locus_tag="ACHY3M_000740"
     CDS             complement(12163..12603)
                     /gene="yadI"
                     /locus_tag="ACHY3M_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4315711.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12708..13478)
                     /gene="yadH"
                     /locus_tag="ACHY3M_000741"
     CDS             complement(12708..13478)
                     /gene="yadH"
                     /locus_tag="ACHY3M_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315712.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13475..14401)
                     /gene="yadG"
                     /locus_tag="ACHY3M_000742"
     CDS             complement(13475..14401)
                     /gene="yadG"
                     /locus_tag="ACHY3M_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4315713.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14510..15172
                     /gene="can"
                     /locus_tag="ACHY3M_000743"
     CDS             14510..15172
                     /gene="can"
                     /locus_tag="ACHY3M_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFS4315714.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15213..15749)
                     /gene="hpt"
                     /locus_tag="ACHY3M_000744"
     CDS             complement(15213..15749)
                     /gene="hpt"
                     /locus_tag="ACHY3M_000744"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4315715.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15955..18345
                     /gene="gcd"
                     /locus_tag="ACHY3M_000745"
     CDS             15955..18345
                     /gene="gcd"
                     /locus_tag="ACHY3M_000745"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFS4315716.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMPGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="ACHY3M_000746"
     CDS             complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="ACHY3M_000746"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFS4315717.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20108..20455
                     /gene="yacC"
                     /locus_tag="ACHY3M_000747"
     CDS             20108..20455
                     /gene="yacC"
                     /locus_tag="ACHY3M_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFS4315718.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20561..21427
                     /gene="speE"
                     /locus_tag="ACHY3M_000748"
     CDS             20561..21427
                     /gene="speE"
                     /locus_tag="ACHY3M_000748"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFS4315719.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21443..22237
                     /gene="speD"
                     /locus_tag="ACHY3M_000749"
     CDS             21443..22237
                     /gene="speD"
                     /locus_tag="ACHY3M_000749"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFS4315720.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22275..22637)
                     /gene="yacL"
                     /locus_tag="ACHY3M_000750"
     CDS             complement(22275..22637)
                     /gene="yacL"
                     /locus_tag="ACHY3M_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFS4315721.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22812..25409)
                     /gene="acnB"
                     /locus_tag="ACHY3M_000751"
     CDS             complement(22812..25409)
                     /gene="acnB"
                     /locus_tag="ACHY3M_000751"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFS4315722.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25764..27617
                     /gene="yacH"
                     /locus_tag="ACHY3M_000752"
     CDS             25764..27617
                     /gene="yacH"
                     /locus_tag="ACHY3M_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFS4315723.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(27688..29112)
                     /gene="lpdA"
                     /locus_tag="ACHY3M_000753"
     CDS             complement(27688..29112)
                     /gene="lpdA"
                     /locus_tag="ACHY3M_000753"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4315724.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29437..31329)
                     /gene="aceF"
                     /locus_tag="ACHY3M_000754"
     CDS             complement(29437..31329)
                     /gene="aceF"
                     /locus_tag="ACHY3M_000754"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4315725.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGVVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDK
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31344..34007)
                     /gene="aceE"
                     /locus_tag="ACHY3M_000755"
     CDS             complement(31344..34007)
                     /gene="aceE"
                     /locus_tag="ACHY3M_000755"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFS4315726.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34168..34932)
                     /gene="pdhR"
                     /locus_tag="ACHY3M_000756"
     CDS             complement(34168..34932)
                     /gene="pdhR"
                     /locus_tag="ACHY3M_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFS4315727.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35473..36846
                     /gene="aroP"
                     /locus_tag="ACHY3M_000757"
     CDS             35473..36846
                     /gene="aroP"
                     /locus_tag="ACHY3M_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFS4315728.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36889..37743)
                     /gene="ampE"
                     /locus_tag="ACHY3M_000758"
     CDS             complement(36889..37743)
                     /gene="ampE"
                     /locus_tag="ACHY3M_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFS4315729.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQMPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37740..38291)
                     /gene="ampD"
                     /locus_tag="ACHY3M_000759"
     CDS             complement(37740..38291)
                     /gene="ampD"
                     /locus_tag="ACHY3M_000759"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFS4315730.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38379..39272
                     /gene="nadC"
                     /locus_tag="ACHY3M_000760"
     CDS             38379..39272
                     /gene="nadC"
                     /locus_tag="ACHY3M_000760"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFS4315731.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39475..39915
                     /gene="ppdD"
                     /locus_tag="ACHY3M_000761"
     CDS             39475..39915
                     /gene="ppdD"
                     /locus_tag="ACHY3M_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFS4315732.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39925..41310
                     /gene="gspE"
                     /locus_tag="ACHY3M_000762"
     CDS             39925..41310
                     /gene="gspE"
                     /locus_tag="ACHY3M_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFS4315733.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41300..42502
                     /gene="hofC"
                     /locus_tag="ACHY3M_000763"
     CDS             41300..42502
                     /gene="hofC"
                     /locus_tag="ACHY3M_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFS4315734.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42537..43580)
                     /gene="guaC"
                     /locus_tag="ACHY3M_000764"
     CDS             complement(42537..43580)
                     /gene="guaC"
                     /locus_tag="ACHY3M_000764"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4315735.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43736..43780)
                     /locus_tag="ACHY3M_000765"
     CDS             complement(43736..43780)
                     /locus_tag="ACHY3M_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315736.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43805..44425
                     /gene="coaE"
                     /locus_tag="ACHY3M_000766"
     CDS             43805..44425
                     /gene="coaE"
                     /locus_tag="ACHY3M_000766"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4315737.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44425..45168
                     /gene="zapD"
                     /locus_tag="ACHY3M_000767"
     CDS             44425..45168
                     /gene="zapD"
                     /locus_tag="ACHY3M_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFS4315738.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45178..45375
                     /gene="yacG"
                     /locus_tag="ACHY3M_000768"
     CDS             45178..45375
                     /gene="yacG"
                     /locus_tag="ACHY3M_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFS4315739.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45476..45865)
                     /gene="mutT"
                     /locus_tag="ACHY3M_000769"
     CDS             complement(45476..45865)
                     /gene="mutT"
                     /locus_tag="ACHY3M_000769"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFS4315740.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(45925..48630)
                     /gene="secA"
                     /locus_tag="ACHY3M_000770"
     CDS             complement(45925..48630)
                     /gene="secA"
                     /locus_tag="ACHY3M_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4315741.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAAPAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48692..49204)
                     /gene="secM"
                     /locus_tag="ACHY3M_000771"
     CDS             complement(48692..49204)
                     /gene="secM"
                     /locus_tag="ACHY3M_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFS4315742.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49435..50352)
                     /gene="lpxC"
                     /locus_tag="ACHY3M_000772"
     CDS             complement(49435..50352)
                     /gene="lpxC"
                     /locus_tag="ACHY3M_000772"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFS4315743.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50453..51604)
                     /gene="ftsZ"
                     /locus_tag="ACHY3M_000773"
     CDS             complement(50453..51604)
                     /gene="ftsZ"
                     /locus_tag="ACHY3M_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4315744.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51665..52927)
                     /gene="ftsA"
                     /locus_tag="ACHY3M_000774"
     CDS             complement(51665..52927)
                     /gene="ftsA"
                     /locus_tag="ACHY3M_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4315745.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52924..53754)
                     /gene="ftsQ"
                     /locus_tag="ACHY3M_000775"
     CDS             complement(52924..53754)
                     /gene="ftsQ"
                     /locus_tag="ACHY3M_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFS4315746.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53756..54676)
                     /gene="ddlB"
                     /locus_tag="ACHY3M_000776"
     CDS             complement(53756..54676)
                     /gene="ddlB"
                     /locus_tag="ACHY3M_000776"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4315747.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54669..56144)
                     /gene="murC"
                     /locus_tag="ACHY3M_000777"
     CDS             complement(54669..56144)
                     /gene="murC"
                     /locus_tag="ACHY3M_000777"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4315748.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56198..57265)
                     /gene="murG"
                     /locus_tag="ACHY3M_000778"
     CDS             complement(56198..57265)
                     /gene="murG"
                     /locus_tag="ACHY3M_000778"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4315749.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57262..58506)
                     /gene="ftsW"
                     /locus_tag="ACHY3M_000779"
     CDS             complement(57262..58506)
                     /gene="ftsW"
                     /locus_tag="ACHY3M_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFS4315750.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58506..59822)
                     /gene="murD"
                     /locus_tag="ACHY3M_000780"
     CDS             complement(58506..59822)
                     /gene="murD"
                     /locus_tag="ACHY3M_000780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4315751.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59825..60907)
                     /gene="mraY"
                     /locus_tag="ACHY3M_000781"
     CDS             complement(59825..60907)
                     /gene="mraY"
                     /locus_tag="ACHY3M_000781"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4315752.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60901..62259)
                     /gene="murF"
                     /locus_tag="ACHY3M_000782"
     CDS             complement(60901..62259)
                     /gene="murF"
                     /locus_tag="ACHY3M_000782"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4315753.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62256..63743)
                     /gene="murE"
                     /locus_tag="ACHY3M_000783"
     CDS             complement(62256..63743)
                     /gene="murE"
                     /locus_tag="ACHY3M_000783"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFS4315754.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63730..65496)
                     /gene="ftsI"
                     /locus_tag="ACHY3M_000784"
     CDS             complement(63730..65496)
                     /gene="ftsI"
                     /locus_tag="ACHY3M_000784"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFS4315755.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65512..65877)
                     /gene="ftsL"
                     /locus_tag="ACHY3M_000785"
     CDS             complement(65512..65877)
                     /gene="ftsL"
                     /locus_tag="ACHY3M_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4315756.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPAVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65874..66815)
                     /gene="rsmH"
                     /locus_tag="ACHY3M_000786"
     CDS             complement(65874..66815)
                     /gene="rsmH"
                     /locus_tag="ACHY3M_000786"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4315757.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66817..67275)
                     /gene="mraZ"
                     /locus_tag="ACHY3M_000787"
     CDS             complement(66817..67275)
                     /gene="mraZ"
                     /locus_tag="ACHY3M_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4315758.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67877..68881)
                     /gene="cra"
                     /locus_tag="ACHY3M_000788"
     CDS             complement(67877..68881)
                     /gene="cra"
                     /locus_tag="ACHY3M_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFS4315759.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68963..69049)
                     /locus_tag="ACHY3M_000789"
     CDS             complement(68963..69049)
                     /locus_tag="ACHY3M_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFS4315760.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(69061..69552)
                     /gene="ilvN"
                     /locus_tag="ACHY3M_000790"
     CDS             complement(69061..69552)
                     /gene="ilvN"
                     /locus_tag="ACHY3M_000790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS4315761.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69555..71279)
                     /gene="ilvI"
                     /locus_tag="ACHY3M_000791"
     CDS             complement(69555..71279)
                     /gene="ilvI"
                     /locus_tag="ACHY3M_000791"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFS4315762.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71398..71442
                     /locus_tag="ACHY3M_000792"
     CDS             71398..71442
                     /locus_tag="ACHY3M_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315763.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(71597..72541)
                     /gene="leuO"
                     /locus_tag="ACHY3M_000793"
     CDS             complement(71597..72541)
                     /gene="leuO"
                     /locus_tag="ACHY3M_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFS4315764.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17944 a        19584 c        17617 g        17681 t
ORIGIN      
        1 aatcccatcg ccatacccta accaattgca cttgtgaatt tatttttgct taattttgaa
       61 ttaagaaaat ttaatttgtt caaaagccct taaagtgcac aataggcgtc ctaatgaaca
      121 atggttgtaa attttggtaa caaatatcat tgctcattaa tatctagcat ctgcttataa
      181 catttgtgtc atgaatagtc attcttcaaa ccaatagtct gactaatgga attttattca
      241 aggattttta tgtctaaaaa attaggtttt gccctgtctg gtcttatgct ggcaatggtt
      301 gcaggtactg cttctgctga tatggatggt ggtcagttaa atatcagtgg tctggttgtt
      361 gataacacct gtgaaacccg cgttgacggc ggtaataaag atggtctgat cctgctgcaa
      421 actgcaactg ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg cgctaaagcc
      481 aaacctttca gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg tagtacagct
      541 aaaatgacct tcggttctgt tttctttggt aatagcaaag gaacgctgaa caacgacatg
      601 tctattaaca acccgtctga cggagtgaat atcgctctgc acaatattga tggttccact
      661 atcaaacagg ttcaaattaa caaccctggc gatgtgtata ctaaagccct ggatgcaacg
      721 acaaaatctg ctgtttatga ttttaaagcg tcttacgttc gtgccgtcgc agaccaaaca
      781 gcaactgctg gttatgtaaa aactaacact gcatacacca ttacttatca gtaagtattc
      841 accaattaaa ttgattgaat gaatatacag ggaatagtgt taataatttc tatttttata
      901 ttattccctg ttttaattaa ctctgtcagg gatggtttat gttttttaac accaaacata
      961 caacagcttt atgcttcgta acctgtatgg cttttagttc atcgtctatt gcggacattg
     1021 tcatttcggg tactcgcgta atatataaaa gcgatcaaaa aagtgtcaac gtacgtctgg
     1081 aaaataaagg gaataacccg ttgcttgtcc agagttggtt agatactggc gatgacaacg
     1141 ctgagcctgg cagtattaca gtccctttta ctgccacgcc gccagtatcg cgtattgatg
     1201 ccaaacgtgg gcaaacaatc aaattaatgt acacagccag cacctcactg cctaaagaca
     1261 gagagagcgt gttctggttt aacgtactgg aagttccacc aaaaccagat gcagaaaagg
     1321 tcgcgaatca aagtctgctg caactggcat ttcgcacacg tataaaactt ttctatcgcc
     1381 cggatggatt gaagggaaat ccctctgaag ccccgttagc cctgaagtgg ttctggtcag
     1441 gttcagaagg taaggcgtca ttgcgagtga ccaatccaac cccttactac gtctctttta
     1501 gcagtggtga tttagaagct agcggtaaac gctatccgat tgatgtgaaa atgattgcac
     1561 catttagtga tgaggtcatg aaagtcactg gccttaatgg caaagcaagt tctgcaaaag
     1621 tacattttta cgccattaat gattttggtg gcgccattga aggtaatgcc agtctgtaat
     1681 caggcaagga tataattccg caggaagcat aacgtgacta taaaaccaac taaaaattat
     1741 cataatcatc tgacccgtat cgctacgttt tgcgcgctgc tgtattgcaa tagtgctttc
     1801 tgtgctgaac ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc ctcaaatatt
     1861 gatctcagcc ggtatagtga aggtaaccct gctataccgg gtatgtacga cgtcagtgtt
     1921 tatgtcaacg atcaacctat tattaaccaa agcattacat ttattgaaat tgaaggaaaa
     1981 aaaaacgcac aagcctgtat cacacttaag aatttattgc agtttcatat aaattctccc
     2041 gatataaata acgaaaaagc cgttctgctt gccagggatg aaacactcgg caattgcctt
     2101 aatttgacgg aaattatccc tcaggcttct gttcgttatg acgttaacga gcaacgtctg
     2161 gatatagacg ttcctcaggc atgggtaatg aaaaattacc aaaactatgt tgatccatcg
     2221 ttatgggaaa atggcattaa tgcggccatg ttgtcataca acctcaacgg atatcatagt
     2281 gaaactcctg gtcgaagaaa tgacagcatt tatgctgctt ttaacggtgg gatgaattta
     2341 ggtgcatggc gactgcgtgc ctcaggcaac tacaactgga tgaccgattc tggcagtaat
     2401 tatgatttta agaatcggta tatacagcgt gatatcgcct cgctgcgttc tcaactcatt
     2461 cttggtgagt cttatacaac gggcgaaacc tttgattctg tcagtatccg cggcattcgt
     2521 ttatacagcg acagccgcat gttgcctccg acattagcga gttttgcgcc tatcattcat
     2581 ggcgttgcta ataccaacgc caaagtcact attacgcaag gtggctataa gatttatgaa
     2641 acgactgtgc cgccaggcgc tttcgtcatt gatgatctga gtccgtcagg ttacggcagc
     2701 gatcttattg ttactgtcga agaatccgat ggctcaaagc ggacattctc gcaacctttt
     2761 tcatcggtcg ttcaaatgtt acgccctggc gttggacgtt gggatattag cggcggccag
     2821 gtcttaaaag atgatattca ggatgagcct aatttatttc aggccagcta ctactacggt
     2881 ctgaataact atctaacggg ttatactggt attcagatta ccgataataa ctataccgct
     2941 ggtttgttag gtcttggtct gaatacttca gtcggtgcat tttctttcga tgtgactcac
     3001 tccaatgttc gtataccgga tgataaaaca tatcagggac aaagttatcg tgtttcctgg
     3061 aacaagttat tcgaagaaac aagtacttca ctgaatatcg cggcctatcg ctattcgaca
     3121 cagaattacc ttggtcttaa tgatgcacta actctaattg atgaagtgaa acatcccgaa
     3181 caagatcttg aaccgaaatc catgcgtaat tactcgcgca tgaaaaatca ggttacggtc
     3241 agtattaacc aaccgctaaa atttgagaaa aaagattacg gttcatttta tctttccgga
     3301 agttggtccg attactgggc ttccggacaa aatcgtagca attactctat tggctacagt
     3361 aacagtgcat cctggggcag ctacagtgtc agtgcccagc gttcatggaa tgaagacggc
     3421 gacactgacg atagcgttta tctcagtttc accattccta ttgaaaaatt acttggcact
     3481 gaacaacgga cttcaggttt ccagagtatt gatactcaaa tgagcagtga ctttaagggt
     3541 aataaccaac tcaacgtcag tagcagtggc tatagtgata acgctcgcgt cagttatagc
     3601 gtaaatactg gctatacgat gaataaagcc agcaaagatt tgagttatgt tgggggttat
     3661 gccagctatg aatcaccgtg gggaaccctg gcaggttcag tttctgcaaa tagcgataac
     3721 agtcgtcaag tgtctctcag caccgacggt ggttttgtat tgcatagcgg cggcctgacc
     3781 ttcagtaatg acagttttag cgactccgat acactggcgg tagttcaggc tccaggtgct
     3841 caaggagcgc gaataaatta tggcaacagt actatcgatc gatggggtta tggtgtcacc
     3901 agcgctcttt ctccttatca tgaaaaccgt atcgcgctgg atatcaacga tcttgagaac
     3961 gatgttgaat taaaaagtac cagtgcagta gctgtaccgc gtcagggttc agtcgtcttt
     4021 gctgattttg aaaccgtgca agggcaatca gccattatga acatcacacg aagtgatggt
     4081 aaaaatattc catttgctgc agatatttat gatgagcaag gcaatgtcat tggtaatgtt
     4141 ggacagggtg gacaagcatt tgttcgtggt attgagcagc agggaaatat cagcattaaa
     4201 tggctcgaag aaagtaaccc cgtaagttgt cttgcgcatt atcaacaaag ttcagaagca
     4261 gaaaaaatag cgcaatctat tattctgaat ggaattcggt gtcagattca gtaactacaa
     4321 gggacgtcaa atgataaaaa taacgccaca taaaatagcg atactgatgg gaatattatt
     4381 atcaccatca gtatttgcaa cggatattaa tgtagagttt acagccactg tcaaagcgac
     4441 aacctgtaac atcacactta ctggtaataa cgtcacgaat gatggcaata ataactacac
     4501 attgagaatc cctaagatgg gtctggataa gatcgcgaat aaaacgacag aatctcaggc
     4561 tgattttaaa ctggttgcta gtgggtgcag cagtggcatc agttggattg ataccactct
     4621 gaccggaaat gcatcatcaa gctcacctaa gcttattata ccgcagtctg gtgattcatc
     4681 ttcgacgaca agtaatatcg gtatgggttt caaaaaacgg actactgatg atgccacttt
     4741 ccttaaacct aacagtgcgg aaaagatacg ctggagcaca gacgagatgc agcccgataa
     4801 gggtcttgaa atgaccgttg cgctacgtga aacagatgca gggcaaggcg taccggggaa
     4861 ttttcgtgca ctggcaacgt ttaatttcat ctatcaataa gaaggataat taagatgaca
     4921 tttaaaaatt tacgttatgg attatccagc agtgtcgttt tggctgcctc attgttcagc
     4981 gtaccctctt atgcggcaac agacagcatt gggctgaccg tgattactac cgtagaaata
     5041 ggtacttgta ccgctacatt agtaaatgac tctgatcagg acatttctgt tgttgacttt
     5101 ggcgatgtat atatttctga aatcaatgcc aagaccaaag ttaaaacatt caaactcaaa
     5161 ttcaaagact gtgcgggtat ccccaataaa aaagcacaaa taaaattaac caagcgagcc
     5221 acatgcgaag gtactgctaa tgacggtgcg gggttcgcaa atggatccac agccgcagat
     5281 aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa ctccggcaac agggagcgca
     5341 acacaattta gttgtgtgac accagcatct caagaggtca ctatctccaa tgcagccaac
     5401 gcggttgttt attatccgat gagtgctcga ctggtcgtcg aaaaaaataa aaccgtaagc
     5461 aatgtcactg cgggtaaatt ttctgcacct gccacattta cagtaaccta taactaacag
     5521 actgacagat ccaggaacta tatatgcatc ccactcaacg taagctgatg aagagaataa
     5581 ttctgtttct gtcactacag tttcccatta cctatccagc catcgctggg caggatattg
     5641 accttgttgc caatgtaaaa aacagcactt gcaaaagcgg aatcagtaac cagggtaata
     5701 ttgatcttgg cgtcgttggg gtgggatatt tttcagataa tgttactcct gaaagctatc
     5761 aaccaggtgg aaaagagttc actgtcacag tatcagactg tgcattacag ggaactggcg
     5821 atgtgcttaa tcagttacat attgatttta gagcccttag cggtgtcatg gctgcaggtt
     5881 caaggcaaat atttgccaat gaagtcgcaa ccggggctaa aaatgtcggt gtcgttctct
     5941 tttccattca ggacccgacc aatatattca atgttattag ttcagcagga aattctcgtt
     6001 ccgtgtatcc agtaatgact tctgccctgc ataattcttc atggaaattt tatgccagaa
     6061 tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca ggtaatgagt catatattag
     6121 ttgatgtgta ctacgaataa tagatcaaaa caaataactg cgactgttac agcagtagat
     6181 ggtggagtga taatgaagaa attcttcaga cattttcttt ttttaattct gtgcttgagt
     6241 tgttataccg cgagtgctgg cactgatgat aacgtaggat atattgttgg gaatagttac
     6301 ggcgttggtc cgtcagatca aaaatggaga gaaacggggc cgaatggcga tgctactgtc
     6361 atattcagat atgcaacaag tacaaataat ttagttttct acaaaccgac gcagcttgga
     6421 cctacaggtg taaaattaca gtggagtcag ttagataccg cttctggtgg tggttttctt
     6481 tattgcaaca gaagtgatag cacaagtggt agcgcaatgc gtattgaaaa tgcaatggtt
     6541 gactcaggta aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt
     6601 acacattatt aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac
     6661 ctgggatata cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag
     6721 acgtgttata ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg
     6781 gtatttatca gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa
     6841 ctcaacagat caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg
     6901 caggctaagg tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg
     6961 tcaagttgac taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt
     7021 ctacggtaaa aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc
     7081 cttttgatat ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt
     7141 caacaaaggt tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg
     7201 cagcaaaagg agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga
     7261 cattgaaacc taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg
     7321 atacaacagg aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc
     7381 aggccacgct acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca
     7441 gtactttcca ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgca
     7501 gtaattgata aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat
     7561 catttatcag gatacgttat gaaaccaacc accattgctt cacttcagaa atgtaagcag
     7621 gataaaaagc gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct
     7681 gatgaaggac ttaacgtcat gctggtaggc gactcgctgg gcatgacggt tcagggtcac
     7741 gactccaccc tgccggtgac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc
     7801 ggcgcgccaa actgcctgct gctggctgat ctgccgttta tggcgtatgc cacgccggaa
     7861 caagcctttg aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag
     7921 ggcggtgagt ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt
     7981 ggtcacttag gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg
     8041 cgcggcgatg aagcgggcga tcaactgctc agcgatgcat tagccttaga agccgctggg
     8101 gcacagctgc tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgatgca
     8161 ctggcgatcc cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg
     8221 atgcacgacg ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc
     8281 gccgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc
     8341 gtttatccgg gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa
     8401 ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc
     8461 tggtgcctac tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag
     8521 cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc aatgcagttt gaccgcccgg
     8581 aagatctggc tcgttatcca cggaccttgc aagaggactg cgaaaaacta aacaaacgta
     8641 aagtggattt agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc
     8701 acacttacgt tgacgttcct agcctttcga ccatgctgga aggtgccagc cgtccgggac
     8761 attttcgcgg cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg
     8821 cctgcttcgg tgaaaaggac tttcagcaac tggcgctaat ccgcaaaatg gttgccgata
     8881 tgggcttcga tattgaaatt gtcggcgtcc cgattatgcg cgccaaagat ggccttgcgc
     8941 tcagttcccg taatggttat ctgacggcgg aacaacgcaa aattgcgccc ggtctgtaca
     9001 aagttttaag ttcaattgcc gacaaattgc aggctgggga acgggatctc gatgaaatta
     9061 ttaccattgc ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc
     9121 gcgatgccga cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag
     9181 ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact
     9241 taactggcgc tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac
     9301 acaggtaact taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat
     9361 ctctgcaacg tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct
     9421 ttcctcctgt cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc
     9481 cagcgtcatc atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc
     9541 cgcttgttca gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc
     9601 gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg
     9661 ctggcgaata tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc
     9721 gtcatccggt gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc
     9781 cagctccggc gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc
     9841 gccctgataa aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg
     9901 ccggtgcatg gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt
     9961 gctttggtgt tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata
    10021 gagcacgtcc gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg
    10081 aagcgtgttg aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc
    10141 gcgagccgtc tccgcgtgca ttaaaaattg tttaaacact gcatcatgcg gtgtggtact
    10201 cggtgcatcc atcttccctg ccttccgtgt tgtcttgcgt gaaggcagta tccagtcaag
    10261 gcgtgccatc gtcagccacc tctttagtgt ttaccgagca gcgttcagag aaatttcaat
    10321 tggttacagc cgttacatgg cacgcttcgg cacggtacgc aaactgctga tgtaaatgcc
    10381 gtcagatgag tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc
    10441 aggcatcgcc tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg
    10501 ctgcagggca aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct
    10561 tgcgccattg accaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat
    10621 atctggaatg tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt
    10681 tcgagaatta tttctgttaa cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc
    10741 atcatcgcca gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc
    10801 gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt
    10861 gcttgattgt tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc
    10921 cgttcttaag atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc
    10981 tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc
    11041 attaaatccg ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc
    11101 gcggcgtgaa cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg
    11161 tcggcgatag ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc
    11221 gcgaatttcg ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg
    11281 cgggtgacgt ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt
    11341 caatacagga tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac
    11401 cgctcgcgca gggagattga tcttattctt cacgtaacct tccagctgcg ccatgctcag
    11461 tgtcgcgtat cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt
    11521 ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt
    11581 tagcaccggc aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc
    11641 gccaatacgg atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg
    11701 gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata
    11761 gaccggcgta tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc
    11821 caaaccgtct tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa
    11881 ccctttacca aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat
    11941 gatttgcccg gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat
    12001 cgcagcattt tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt
    12061 aaacagcatc agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa
    12121 acaacgcttt tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt
    12181 atgtttgagg acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg
    12241 cccacccagt tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc
    12301 catcatctgt tcaattaacg gtaacgtgac accagaaatc acttcacaac gggagtgttt
    12361 atggcttaat aatgaagcca cgcggtacgg tggcgctccg gctaaatcgg ttaagaagat
    12421 gacaccctca ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt
    12481 agagcttaat ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt
    12541 ctccagcgca tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa
    12601 catcgccttc ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat
    12661 agccgtcaaa actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa
    12721 ccacgtccac gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc
    12781 agtacgccaa aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa
    12841 ccactgatca tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac
    12901 aaagtcaggg agtaaaacac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc
    12961 accaggctga tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag
    13021 aatagcaccg ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac
    13081 ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg
    13141 ccaccgccga catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct
    13201 tcaatattac gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcattg
    13261 gtgatcaccg acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc
    13321 atatcgccaa tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg
    13381 atgactggcg gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc
    13441 cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat
    13501 taaccagtga aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct
    13561 gaatgccctg ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt
    13621 caacttccag cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg
    13681 gacttttcgg tgcgagatcg agaataaagg tttccgattt cagcttagcc agcagcgcct
    13741 tcatcgaggt attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca
    13801 tttctgcttc ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat
    13861 ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt
    13921 cgagaatcag tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca
    13981 tcccgccaga taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agctgcttaa
    14041 gatatttttc gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat
    14101 tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc
    14161 ccaactgacg tttagcgttc acgacatcct tctcgagatc gtaaccaaat acgctgaccc
    14221 gcccggaggt tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc
    14281 cgttcggccc gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct atcccacgaa
    14341 gcgcctgaac gccgcctgga taggtttttt taagctgttg aagttccagt gcaatggtca
    14401 taaattttta cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt
    14461 gccctatatt agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat
    14521 agatacactc atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg
    14581 gttttttgag aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga
    14641 cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg
    14701 taatgttgct aacctggtca ttcacactga cctgaactgc ctttccgtgg ttcagtatgc
    14761 agtggatgta ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt
    14821 acaagccgca gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg
    14881 cgatatatgg ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga
    14941 taccttgtgt gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat
    15001 gcaatcagcg tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca
    15061 cgacggcttg ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg
    15121 ttaccgtcac gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca
    15181 tgccggatgc aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg
    15241 atatacggca gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca
    15301 tccgggatcg agaaaccgat aaattctacc gggacgttca cttcacgacg ggacggttta
    15361 tccagcagcg tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact
    15421 ttcgacagtg tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg
    15481 atatcttcat ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg
    15541 tagctggagg cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc
    15601 gccataaaca taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct
    15661 ttgtaacgct cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg
    15721 gggatcatta cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca
    15781 cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa
    15841 actattcatt cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta
    15901 aaacctataa ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca
    15961 attaacaata caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt
    16021 tgcgggctgt atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac
    16081 taccctatcg ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt
    16141 gccgcgcttt ggctatacgc cgccctgctg ctcggcacca tgatttgggg cgtctgggaa
    16201 gttggtttcg acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc
    16261 tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc
    16321 gcactggtgg tggcactgct gattagcggt agtatcctga cctgggccgg atttaacgat
    16381 ccgcaggaaa tcaacggcac cttaagcgcc gatgccacac ctgcggaagc tatctcaccc
    16441 gttgccgatc aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct
    16501 ctgaaacaaa ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact
    16561 ggcgatgtga agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa
    16621 gtgggcgaca ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc
    16681 agcggcaaag agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac
    16741 gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg
    16801 atggcggatt gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt
    16861 aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa
    16921 agcaatatgc cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc
    16981 gataaaacca tcgtgatggc aggttcagtt accgataact tctcaacccg cgaaacgtct
    17041 ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc
    17101 gcgaaagatc cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac
    17161 tcgtgggcgc cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg
    17221 accacgccgg atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg
    17281 attctggcgc tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac
    17341 gacctgtggg acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt
    17401 cagaaagtgc cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt
    17461 cgcaatggcg aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa
    17521 ggcgattacg ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat
    17581 ttgagcggtg cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg
    17641 ttccaccaga tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc
    17701 ttcccgggta acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa
    17761 gtggcaattg ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg
    17821 ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag
    17881 ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg
    17941 ccgtgtaaac agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg
    18001 gtgtggaaga aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg
    18061 gtgccgttca atatgggtat gccgatgccg ggcgggccaa tctccacggc gggtaacgtg
    18121 ctgtttatcg ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa
    18181 aaactgtggc agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg
    18241 aatggcaagc agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg
    18301 ggcgactata ttgtggctta tgcgctgccg gatgatgtga aataaccata atgcccggag
    18361 agatccgggc atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg
    18421 tatcttcatg ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt
    18481 gattaaactt caccagcact tcactgacat tgccttctac cttaacggta tctttccagc
    18541 ccgcgcgatg agccgctggc ggtttgccat tttctgacaa gatacggaac tgggtgccgt
    18601 gaatatggaa cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact
    18661 gccctttcgc cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat
    18721 tggcatggtg gaaatcgaac ttcccgccat ggttcatatg attcatattg ccgtgcccca
    18781 tatggcccat catctggcta tgatccatcc cggccatcgc ctgatcgcca tatttctcca
    18841 tcagcatctg catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta
    18901 ccgtcagccc ttccagagaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac
    18961 cggaggcact aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg
    19021 caatcgccat ccccatctgg ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt
    19081 taacctccac cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca
    19141 ccggttcagg tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg
    19201 tggcgaaatt gagcgagcgg gcattacagc cattaagcaa acgcaggcgc agccaaccac
    19261 gcggggcagc gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc
    19321 ccacggcggc ggtcatcaca tccagttgat aatcaatctg cccatcagcg ttaaattttt
    19381 tatcctgaac aatcaccgga acatcatcga tgccccactg ttttggtagc attaatttca
    19441 ggatctcgtc atcttcaatt accaccagcc cagccaaccc catcgccacc tgtcgcccgg
    19501 tcttgccatg ttgatgtgga tggaaccagc aagtagcggc aggttgatca acgttcaacg
    19561 tcaccgagcg cttgccacct ggcagaataa ttccctgcgg gccgccgtcg acttcacccg
    19621 gtacttccag cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa
    19681 ccgttaccgc tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc
    19741 cccaggtagt tgcagttttc tcgccaaagg tagactgacc tgcgccaata gttaactgaa
    19801 tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg
    19861 caaagactgc gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt
    19921 ttaagaaatc acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca
    19981 aacgttgagc atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga
    20041 caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt
    20101 agaagcaatg aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc
    20161 aaacagttac gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt
    20221 tgtctttctg aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc
    20281 actggtcttt ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat
    20341 gaaagccctc ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa
    20401 aaagtgcaaa gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct
    20461 gatacctttt tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc
    20521 gccctttttt tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg
    20581 gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca
    20641 tgaaaagacc gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat
    20701 ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat
    20761 gacccatgtt ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg
    20821 cgacggtgcc atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt
    20881 ggaaatcgat gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg
    20941 tagctacgac gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca
    21001 aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga
    21061 aagtctgttc acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat
    21121 cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg
    21181 caaactcagc cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta
    21241 cggcggtatc atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac
    21301 cgaaattatt caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc
    21361 aatccatacg gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc
    21421 gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac
    21481 caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga
    21541 cggttatatt gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc
    21601 agaaacctgt tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc
    21661 acagggtgcc agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga
    21721 caaaacagaa caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca
    21781 tatttgcgta catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc
    21841 cgatattgaa gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat
    21901 ccaccagctt gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga
    21961 cattaacggt atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc
    22021 tgacgatatg aaggcgctgt atgacatggt ggatgtgaac gtctatcagg aaaatatctt
    22081 ccataccaag atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc
    22141 ggaagattta accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg
    22201 cgaaatttat tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc
    22261 tccgccagat ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga
    22321 aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc
    22381 atttcatcgc cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc
    22441 cacagggtgt attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc
    22501 gctgcttgtt ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag
    22561 tgcccgacca cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc
    22621 agaaattcgt aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc
    22681 gccattgatg taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag
    22741 aaataaaaaa tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt
    22801 gcgcttactt attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga
    22861 gaaagctggt tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca
    22921 acgtaggtct ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc
    22981 agttctgcag aagccaggaa gacattcgcg ccagtcccca gacggttcgg gaagttacgg
    23041 gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg
    23101 gaacagccag ggatctcgat acgcgcaccg ctcttaccga agacgctata gtagccttct
    23161 tcggtcaact gcgcggcatc catacgggtt ggcggtgcca cccacaggcg cgtcggcaac
    23221 tggcctttgt gcgcatccag cagtttaccc gcagcacgga agtgaccgat gttggtcatg
    23281 caggaaccga taaacacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg
    23341 tcgtccgggt cgttcggtgc acagaggatt ggctctttaa tatccgccag atcgatgtcg
    23401 atcactgccg cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat
    23461 ttttccatgc cctgaatacg acgttccagg gtacgacgat cgccgtaacc ttccgcgatc
    23521 atccacttca gcaggacgat gttagagttc aggtattcga tgatcggttc tttgttcagc
    23581 ttgatggtac aaccagcggc agaacgctcg gcggacgcat cggtcagttc aaatgcctgc
    23641 tcaactttca gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc
    23701 ttgcctttct tctcaacggt cagcagacct tgtttgatcg catacagcgg gatcgcgtgt
    23761 accagatcgc gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat
    23821 tccggcatat ccagcggcat tacgcctgtt gcggctgcaa atgccaccag accagaaccc
    23881 gccgggaaag agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta
    23941 tccggcagca gcatacggtt cagccaggag tggattacgc cgtcacccgg acgcagcgac
    24001 acaccaccac ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc
    24061 ttcggatacg ccgcggtatg gcagaaagac tgcatcacca ggtcagccga gaagcccagg
    24121 cacgccaggt ctttcagttc atcacgggtc atcgggccgg tggtgtcctg tgaacctaca
    24181 gaagtcattt tcggttcgca gtacgcgccc ggacgaatac ctttcacacc gcaggcacga
    24241 cctaccattt tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga
    24301 cggaacacat cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc
    24361 ccgataatca gcgggatacg gccgccagca cgcacttcat caatcagcac gtcggttttc
    24421 agttcgaagg tcgccagcag ttcgccggtt tcgtggttac gcacttcacc tttgtacggg
    24481 taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt cgacttcgat tggcagtgca
    24541 cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca
    24601 ccgccgcgtt tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc
    24661 gtggcggatt tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa
    24721 cctttctgtt gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc
    24781 tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgc
    24841 gaccacgcat ccggtgccgg agaaaggtca tcggtgttag tttcgccagt gactttgaag
    24901 acagtaacgg tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc
    24961 caggactgca taacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca
    25021 tagaagttat cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt
    25081 ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc
    25141 agcagttcga tggctttttc cggagtcagc agaggggatt tggcttcgcc tttcgcgata
    25201 gcagccagga agcctgcttt gacataggcg gcttcatcga cgcctggggg aacacggttg
    25261 gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca
    25321 agtgcggcca tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca
    25381 gctacgtgct tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg
    25441 cggcaggcga ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta
    25501 ccgcaacggg cagaatagca ggattttgac gatgtgttaa tctgtttaca aaaaagcaac
    25561 ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt
    25621 cgttaattcg catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta
    25681 aactaccgcc tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt
    25741 gcgatttcct tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg
    25801 cgctaatttg cagtgccggg ctttgtgccg cctctaccgg gctatatata aaaagccgca
    25861 cagtggaagc gcctgtggaa ccgcaatcga cacaacagac tgcgcctgac atcaccgcag
    25921 ttacgcttcc tgcaacggtt tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat
    25981 tcagcactgc acaaatcgat caatgggttg cgcctgtcgc gctgtacccc gattctctgc
    26041 tttcacaagt gttaatggca tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt
    26101 cgcacgataa tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt
    26161 gggacgccag cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa
    26221 acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg
    26281 actcggtaca gcgattgcgg caactggcgc aacaaaccgg ttcgctgaag tcatcaaccg
    26341 aacaaaaagt tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac
    26401 ccgtcatacc atccaatacc gttttaactg ccagccccgt cattacagag cctgcaacaa
    26461 ccgtcatttc cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa
    26521 ccgtggttta cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag
    26581 ccggagaacc gtttattgac agctttgtgc gcggattcgg ctatagcatg ggtgttgcta
    26641 ccacgtacgc actattcagc agcatcgact gggatgacga cgatcatgac catcatcatc
    26701 atgacgatga tgattatcat caccacgatg gcggtcatcg tgacggtaat ggctggcaac
    26761 acaacggcga caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc
    26821 ttactgataa gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc
    26881 atgatcagga tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagcgcca
    26941 cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc
    27001 aacgaacaca cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc
    27061 ggtttaatga agctgaaaac tatgggagct atgacgactt ccgcgacttc agccgtcgcc
    27121 aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg ttatcagtcg gcctcgcctg
    27181 agcagcgcca ggcagtccgc gagaaaatgc aaactaaccc acagatccag cagcgaagag
    27241 acgcagcgcg tgagcgtatt cagtccgcct cgcctgagca gcgccaggca gtccgcgaga
    27301 aaatgcagac taacccacag atccagcagc gaagagacgc agcgcgtgag cgtattcagt
    27361 cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc
    27421 aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc atcacctgaa caacgtcagg
    27481 tttttcggga aaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg
    27541 tcaggctgaa taacgagcaa cggtcagcag tacgcgaacg tctctctgag cgcggagcaa
    27601 ggcgacagga aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa
    27661 ttgccggatg ttccggcaaa cgaacaatta cttcttcttc gctttcgggt tcggcaggtc
    27721 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    27781 cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat
    27841 ttcacccagc agctcgccgc cgttggtacc gacaatcgca ccaccgatca cacggtgaga
    27901 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcggaagcga tagcacgacc
    27961 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    28021 ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    28081 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    28141 acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tatgcggtac
    28201 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtccactt ccacgccagc
    28261 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc
    28321 gtaacgctgc ggttcagcgg gtgctttttt gccttccatc gtcacataaa taccgtcttc
    28381 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg
    28441 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    28501 aaccacgtca atctgtgaac ccagcgcgtg gtatacggtg cccatttcca gaccgatgat
    28561 accgccaccc attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga
    28621 gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc
    28681 tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag
    28741 ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    28801 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    28861 gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    28921 ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    28981 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    29041 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    29101 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    29161 ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg
    29221 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    29281 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    29341 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    29401 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    29461 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    29521 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    29581 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    29641 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    29701 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    29761 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    29821 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    29881 ttccgacaac gaactgttga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    29941 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    30001 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtgag taacatgcgg
    30061 gatcatcacc cagttacggc tcaggttcgc accagaaatt ttctggatac ggcccagttc
    30121 cacttcttcg atttcaccaa acttgctgaa gtccaccttc ggccacggca gcatgcctgg
    30181 gataccgccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    30241 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    30301 gttaacgcca aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa cgtaagcgtc
    30361 gttttctgca aattcagatt tgccttccgc tttcgcagcc ggtgctgctg ccggagcttc
    30421 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagccg ccgcaggcgc
    30481 tgcgccttca acttcgaaga tcataatcag cgagccagtt ttcactttat cgccaacgtt
    30541 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttgtc
    30601 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    30661 ttcggtcact tcaactttgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    30721 cgctggtgca ggggccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc
    30781 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    30841 gttcactttg atctctttca cggtgccagc aaacggagcc ggaacttcca tagacgcttt
    30901 atcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    30961 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    31021 tgcagccgct ggtgctgctg ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    31081 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    31141 atcgccaaca gagactttga tctctttaac gacacccgcc tgcggagacg gaacttccat
    31201 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    31261 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    31321 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    31381 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    31441 tcgcccagcg ccgctacaac cacgtaggaa gcatcaactt cgaagtggtg acgcaggttc
    31501 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt
    31561 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccgga
    31621 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg gtgcagcatg
    31681 ttccagcgtt cacaatcctg accatctcgc gccagttcgg tgaaggaggt cacgctataa
    31741 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    31801 aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    31861 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    31921 ttttcgttca gcgtggtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    31981 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    32041 cagttcggaa tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttcg
    32101 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg
    32161 ccagccgccc agcacagatc gccaatacgc tggaagccga acatcgagta atagatgtag
    32221 aatgggatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacaa
    32281 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    32341 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    32401 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    32461 agacgatctt tgatcgactt gttcttcagc attacgttca gggcacgaac gaaagcgata
    32521 gtggtagaga tctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    32581 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    32641 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    32701 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    32761 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    32821 ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct
    32881 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    32941 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    33001 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    33061 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    33121 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    33181 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    33241 aagaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    33301 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    33361 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggccccaga
    33421 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    33481 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga
    33541 ccttccagga aagcacgagc gtacacgccc ggggagatgt ggccctggaa gtaaaccagg
    33601 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    33661 gttgcggaag actggaagga cgccatatgg ccgcccagtt cgaggtcttt tttcgacgca
    33721 cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    33781 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    33841 cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    33901 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    33961 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    34021 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc
    34081 aataagacag gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct
    34141 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    34201 acgctcacgg cggctctctt cacgacttct gtcgagcaaa atttcttcga taaaggccag
    34261 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    34321 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    34381 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    34441 cacattgtgg gccgcttcag tgacagcaat ctgatactgg agtacggcgt ttgattccgc
    34501 gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc
    34561 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    34621 tcgtgtttcg agcaagtcat actgtgactc aggatggtcg gagagcagct ccaccagcgg
    34681 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    34741 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    34801 aaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    34861 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    34921 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    34981 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    35041 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    35101 tatgcacaaa tgtagacttt acgtaggaaa ggagtttttt aacgattatt aaactataaa
    35161 aatccgaatt gaaccgattc acttaccaat tttgtgattt ttaattcaat taaaacgaat
    35221 ttaaattcat tctacatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
    35281 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgcctcat accattattc
    35341 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct ttgatgtaaa caaattaata
    35401 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaaccacc
    35461 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa
    35521 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggaccgggtt attcctgggt
    35581 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt
    35641 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
    35701 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
    35761 tggaactact gggtactgta cgttttagtt gccatggctg agctgactgc cgtgggtaaa
    35821 tacattcagt tctggtatcc ggaaatcccc acctgggttt ctgccgccgt attctttgtg
    35881 gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt
    35941 gccattatca aagttatcgc ggtggtagcg atgatcatct tcggcggctg gctgctattc
    36001 agtggcaacg gcggcccgca ggcgaccgtt agcaacctgt gggatcaggg tggtttcctg
    36061 ccgcacggct tcaccgggct ggtgatgatg atggcgatta tcatgttctc gttcggtggt
    36121 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tataccgaaa
    36181 gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
    36241 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
    36301 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
    36361 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
    36421 cagggtaatg cgccaaaagc gctggcgtct gtcgataaac gtggtgtacc agtaaatacc
    36481 attctggtgt ctgcactggt aacggcgttg tgcgtactga ttaactacct tgccccagag
    36541 tccgctttcg gactgttaat ggcgctggtg gtatctgcac tggtaatcaa ctgggcgatg
    36601 attagcctgg cgcatatgaa attccgtcgc gccaagcagg aacaaggcgt ggtaactcgc
    36661 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
    36721 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
    36781 ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg
    36841 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatcttt acaccaacgc
    36901 cccgtaaatc gtcagtagtg caatcaccac cacgaccacg aacgaggttt tcttcgccat
    36961 tgaaaccgct gccctcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
    37021 ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg aaatcaccca gcgaagcaaa
    37081 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
    37141 aaccggcacc caatccagta catgaagcac ggcatcaatg ccggactgta aacgatgatg
    37201 cggcatctga tatcgcgcca gccagtattg ccatgcacgc aaaaacgcat accccatcag
    37261 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
    37321 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
    37381 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
    37441 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
    37501 acacagcaaa ccaatcagca gccacaccag tagcgtggga acgttgaaca atactccctg
    37561 caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata atggtcatgc ctaacgtgcg
    37621 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
    37681 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
    37741 catgttgtct ccttgctgac cagcacacga aaccgtgccc aatcaaatgc aggaccggga
    37801 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgtttttagc gatatccgga
    37861 tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac
    37921 gccagcgtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcgcgc
    37981 ccctgatact gagagactcc cgcatgccat gcacgtttat cgaaaggaac atactggact
    38041 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
    38101 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
    38161 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
    38221 tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt
    38281 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
    38341 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctataacc
    38401 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtag
    38461 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgat attacggcaa
    38521 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtct
    38581 tttgcggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
    38641 taatctggca tgtggatgac ggcgatgtca tcaatgccaa tcaatccttg ttcgaacttg
    38701 aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc
    38761 tttcaggagt tgccagtaag gtacgccact atgtcgaatt gctggaaggc accaacacgc
    38821 agttgttgga tacgcgcaaa accttacccg gcctgcgttc agctctgaaa tacgcggtac
    38881 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
    38941 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcacc
    39001 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgaa gccctgaaag
    39061 caggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
    39121 aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
    39181 gtgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
    39241 aagcactcga cctttcaatg cgttttcgct aatagttgac agatttatcc gctccatcgc
    39301 gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
    39361 cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc
    39421 ctgtgctgat ggcgtttgcc aaagtagcgc caactaaatc aaggagcgaa acagatggac
    39481 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
    39541 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
    39601 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
    39661 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
    39721 tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
    39781 ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg gcgtcaccgg ctggacgcgc
    39841 aactgcaata ttcaaagtga cagcgcattg catcaagcct gcgaagatgt cttccgcttt
    39901 gatgacgcca actaaggagc ggcaatgaat attccacagc tcactgccct gtgtctgcgt
    39961 tatcatggag tcttgctgga tgccagcgaa gaggtggttc atgttgcggt agtcgatgca
    40021 ccttcgcatg agctactgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
    40081 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
    40141 gctgttcagg agaagcatca gcccaaagca gagttgctaa ctcgaacgtt acaatctgcg
    40201 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
    40261 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
    40321 ttaaccgcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
    40381 gacgggcaat tcactgtcga actggcagga aacgccgtct catttcgtat tgcgacctta
    40441 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg
    40501 gatgtcaaca cgcttggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
    40561 caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt
    40621 tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg
    40681 gttgagatcc ctatagccgg actaaaccag acgcaaatcc atccgcgtgc cggactcacc
    40741 tttcagggcg tgttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
    40801 atccgcgatg gcgaaacagc agagatcgct attaaagcgg cgcaaactgg tcacctggtg
    40861 ttgtctaccc tacacactaa ttccacctgc gaaacgctgg tacgtttaca gcaaatgggg
    40921 gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgc
    40981 aaactttgcc cacattgtcg ccggcagcaa ggggagccca tccacattcc agacaatgta
    41041 tggccatcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    41101 tatggtcgta cggccttatt tgaagttctg cccataacgc cggtcattcg tcagcttatt
    41161 tccgctaata ccgacgttga atcgctggaa acgcacgcac gacaggcggg tatgcgtacg
    41221 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    41281 cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg
    41341 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtgctttgc tgctcatggc
    41401 actacagcaa cagatggtta ccccacttag cctgaagcga atcgccatca attctgcgca
    41461 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac tcaaagccgg
    41521 gttaacgctt tctgaagggc tggcactgct ggcggaacag catcccagta agcaatggca
    41581 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    41641 attaccctgg tcagaggtat ttccgccact ctatcaggcg atgatccgca cgggtgaact
    41701 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaag cccagcgtca
    41761 gttgaccgac aaagtgaaat cagcgttacg ttatcccatc atcattttag cgatggcaat
    41821 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    41881 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    41941 cgaatggagc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    42001 gatgcgccga ccgacctggc ttatagtgcg gcagaaattg ctgttacgca tcccgattat
    42061 gggttcactg atgcggggac aaaaactcac gcagattttt acgattctgg cgctgacaca
    42121 aagtgcaggc attacttttt tgcagggcgt agagagcgtc agagaaacaa tgcgctgccc
    42181 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    42241 gctggcgcta aaaaataccg gggagtttag cccgctctgt ttgcaattag tgagaacagg
    42301 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    42361 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    42421 gggaggaatt atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga
    42481 tgcgatgagt gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac
    42541 aggttgttga agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc
    42601 agttctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    42661 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct
    42721 gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    42781 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    42841 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc ttttgcgaca
    42901 tcgcccggcg tggtgcagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    42961 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    43021 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    43081 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    43141 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    43201 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    43261 tcagacgtac cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    43321 aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa
    43381 gccagcgcag aggccataga aaatgtacct acggtgtcca tatttgcggc gataattggc
    43441 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    43501 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    43561 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    43621 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    43681 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    43741 ttcgctttcg tgcactcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    43801 aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc
    43861 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    43921 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaacatgat
    43981 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccaga
    44041 agagaaaaac tggcttaacg ccctgctgca tccgctgatt cagcaagaga cgcaacacca
    44101 gatccagcaa gctacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc
    44161 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    44221 gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca
    44281 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    44341 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    44401 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    44461 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    44521 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    44581 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt
    44641 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    44701 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    44761 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    44821 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    44881 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    44941 gatttaattc gccagtcggc tcccttccgt aaacaaacca gcctgaatgg tttttatcag
    45001 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    45061 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    45121 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtgaaaatg
    45181 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    45241 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    45301 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    45361 gagccaaagc agtgacattt gtctgatgcc gcacgtagat actgataaga cgcggacagc
    45421 gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca
    45481 gacgcttaag cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac
    45541 cgactagcga catccactca ccgggttgcc cttctttacc ccacggttcc ccttcccagc
    45601 tttcgaccag ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt
    45661 caaatagcga aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca cgcaccaccg
    45721 cctgttccgg cgtttcgccc atttcaattt taccgccggg aaactccagt ttattcgcca
    45781 tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt gttctcgttg cgaataatac
    45841 ctaccgcaat ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc
    45901 gccttttact tcaactgtta gcttttattg caggcgacca tggcactgct tgtatttttt
    45961 accagaaccg cacgggcaag gatcgttacg tcctactttg cgctcaccgg tttgcgccgc
    46021 cggtgcagct gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgcgctaa
    46081 acgctcggct tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac
    46141 ctggactttg ctcagcgtgc tgataacttc atattttaac gactccagca tcgctgcaaa
    46201 catggagaac gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag
    46261 gtggataccc tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag
    46321 cgtttgcagc atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc
    46381 tttacgctga tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg
    46441 cagttctggt tctttatcca gccactcggc aattggtaaa tcgaggtcga aatcgttctt
    46501 cagacgttcc tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta
    46561 ggcatcaatg gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc
    46621 gctgacatcc agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac
    46681 gtcatcatat tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg
    46741 ggcgttggca atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc
    46801 cagtttacgc atcatgccgg atactcggtc ggaagcaaaa atacgcatca gcgcatcttc
    46861 catcgacagg tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa
    46921 ctggttatcg atacgacggg attcgtgacg ctcggtaccg atgatatgca ggccacctgc
    46981 ttccagtacc gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctac
    47041 ggtcggattt tccagcgcgg caacttctgc ctgccagcta ccaccgagca caatatctgt
    47101 accacgaccc gccatattgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac
    47161 aatcgccgct tcgttggcgt ggaatttggc gttcaggacg ttgtgcttaa taccggcttt
    47221 ggtcagttcg tttgacacca gctccgattt ttcgatggag atagtaccca ccagcaccgg
    47281 ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc
    47341 agtcatgtag accaggtccg gcagatcttt acgaatcatt ggacggttgg tcggaacaac
    47401 gacggtatcc agcttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt
    47461 catccccgcc agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt
    47521 ttggttttcg ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc
    47581 ggaccagcga cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc
    47641 atctttaacg atgtagtcga cgtcgcgggt aaacagcgca tgagcgcgca gcgccgccgt
    47701 tacgtggtgc atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat
    47761 gccctctttc accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg
    47821 gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt
    47881 ttcctgacgg atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc
    47941 ttctgccggg ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac
    48001 ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat
    48061 attgtcgcgc aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc
    48121 gtaagcttcg cgctttgccg gtgctggcat gcccggcagg ttgataccga cagtcaggcc
    48181 aaggaattca aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac
    48241 ggtaactacg tgcacgcctt taccggttag tgcgttcagg taagcaggca gcgttgcggt
    48301 cagggtttta ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc
    48361 gccgagtaac tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgtac
    48421 cacggcgaaa gcttccggga tcagattttc cagcacttcg cctttttcca gacgtgcacg
    48481 aaactctgcg gttttccctt tcagttcttc gtcggagagt ttttccatct ccggttccat
    48541 ggcattgatg atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc
    48601 gaaaacttta gttaacaatt tgattagcat aataaaatct caaacgcccc gcgttgcgga
    48661 gttaataaaa tgaagtaaag gtttattgtt gttaggtgag gcgttgaggg ccagcacgga
    48721 tgccttgcgc ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg
    48781 cataatcaat gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga
    48841 gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca
    48901 gtgtttgcgg tgccattgcg aaagaaagat gacggattac cgtgcgaatg gcatgttgat
    48961 gccagtaatc aacggaatag ttcgaattcg ggcggcgtgt gttcgcttcc agcaaggcca
    49021 attgaccaaa gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg
    49081 cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa actcgccgca accatcccta
    49141 ataagagatg cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac
    49201 tcacgttatt gccatcccga tcgtattcaa acgtatttag cgcacaaatc ttatcattaa
    49261 agcctgcagt ccgcagcacg aatgaagata agttgtgcgg aaaagtgctt aagaaaacag
    49321 cgttcgcacc gttttttata gctcttataa aatttaaaaa aaaacgactg gttcacctgg
    49381 ccggagagag tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc
    49441 cagtacagct gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac
    49501 atattcccag gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg
    49561 accggattta taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc
    49621 gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt
    49681 cagtacgcga taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc
    49741 acgggactgc agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg
    49801 gcgcataaac gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc
    49861 cggatggtta aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc
    49921 ccacttatcg ccatcttcga cacgaacagt ctctttgatg cgaacaaatt ttttggcgca
    49981 gttcaactcg tcgataccgg cgtcaagcag caagtataca aacggagcgg cgctgccgtc
    50041 catgatcggg atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaagcccgc
    50101 gagagcagca ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca
    50161 cgtacagagc atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt
    50221 caagtcggtg cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt
    50281 gactttcttg ccggtatgta agccgacacc cgtcgcctga acgatacgtt taagtgtcct
    50341 ttgtttgatc atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt
    50401 cggcgggcca gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag
    50461 cttgcttacg caggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg
    50521 cattgtcatt cacgacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccat
    50581 gctgctggta gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt
    50641 caggacgttt gtccatgccg atacctgtcg caacaacggt tacgcgcagc tcgtcattca
    50701 tatccgggtc aagagaagta ccgataacca cagtcgcgtt gtcggaagca aatgcacgga
    50761 tggtgttacc taccgtttcg aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa
    50821 ccagcacgcc gcgcgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca
    50881 tttcagcagc ttcttccgca cggtcttcac cgctcgccac gccagaaccc atcattgcgt
    50941 agcccatctc agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac
    51001 gagtaatcag ttcagcgata ccttgcacag cgcctttcag tacatcgttc gctgcgccaa
    51061 acgcatccag cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag
    51121 tgatcagaga gtccacatgc ttggacagtt cagtgatccc ctgctccgcg aatgccatac
    51181 gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc aacggtcagg atacccaaat
    51241 cttttgccac ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg
    51301 cagcaataaa gaccatgtct gcaccttcca gcgccgcacg caatgcatcg cgatcctcat
    51361 cagccgcatt gcggccaact tctggattag cgccagcgcc cagtcctttg gtgataccgc
    51421 taccgatttg aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta
    51481 ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca gcattaccgc
    51541 cgccgccgcc gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa
    51601 acatagtttc tctccgattt gtgcctgtcg cctgaggccg taatcatcgt cggcctcata
    51661 aaaattaaaa ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg
    51721 ctgtaacacg tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa
    51781 gcaatcccac cgccgtcgaa taatacggct cctgagcata atccgttaaa ccggtaatgt
    51841 tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac
    51901 cttcgatctg cgctgcgcca ccggttaata caatgcctgc cgccaggtga tgtttaaccc
    51961 cttgttggcg aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcagct
    52021 cggtatagcg cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac
    52081 gaccacctac gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac
    52141 aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga
    52201 tatcactggt cacgacattg ccagcataag gaattacctt agtgtggcgc aatgccccac
    52261 cggtataaac ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt
    52321 cacgttcatc ttccgtcaat accgaataac ttgatgccag tccggcaaat atcagttggt
    52381 caactttcag cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac
    52441 atgtgatcag gtgcactttt gcctgcatcc gcacgcccga aagtcctacc ggattcttga
    52501 tcccttcctg atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat
    52561 cgcgcacacg caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt
    52621 cttcttcaga aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa
    52681 gcgccagata taccgaagag atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc
    52741 gttgtacgca cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgtg
    52801 acgggcagct gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta
    52861 aagcggcaac cttcgcggta ccaatctcca gtcctactac cagttttctg tccgtcgcct
    52921 tgatcattgt tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggcggcaa
    52981 gggcgcccag cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt
    53041 gccatcggtt tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gagccaaacg
    53101 tttcatcgta tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca
    53161 agaacgccgc gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg
    53221 ccccatttcg cgataaccct gcaacacttc attggcgctg ccttccgggc catacagcat
    53281 tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg aaggtgtttc cttccgcgtc
    53341 taccatatgt tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa
    53401 ttcatcaggc cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat
    53461 ttgcgtctgg atgatgttga catcttgggt cataaaggta cccggctcac ccaatgccag
    53521 gatcgactgc cggatatcgt cattacgcgt gtaatggcgt tcaccggtca acaccagctt
    53581 tgagagcggc aggcgttgcg catcttccat ccagcccaac acgacccagc cgctcaccaa
    53641 cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg
    53701 gcgagaagaa acctcttctt cgctatttcg cgtgttcaga gcagcctgcg acatattagt
    53761 ccgccagttc cagaattcgt actaccaact gcgagaagct catacctgcc tgacgtgccg
    53821 ccatcggcac caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa
    53881 actgtccatc gctgtccagc ataacatcaa tacgtcccca tcctttgcaa cctaacgtcg
    53941 tccatgcttt cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg
    54001 ggcagaaata ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg
    54061 acggttgaat acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg
    54121 gcccacttag ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg
    54181 catcttgtag agcattttct gctactactt ttgacattcc cacactggaa ccttcgcggc
    54241 tcggcttaac gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcgctca
    54301 ggcctttttc aaactctgcg cgggttaacg ctacccacgg cgcgaccggt aaaccggcac
    54361 cttgccacag cagtttgctg cgtagtttat ccattgaaag cgcagatgcc atcactccgc
    54421 tgccggtata aggcaagccc atcagctcga gcatgccctg cagcgtacca tcttcaccgc
    54481 cgcgaccgtg tagcgcgata aacactttct gaaaacccat cgacttcagt tgcgtcacgt
    54541 cgacttcttt cgggtcgaca ggatacgcat caataccgcc ttcacgcagc ccggctaaca
    54601 ccgctgcgcc agaattcaga gaaacttccc gctcagcgga agtcccgccc aacaggaccg
    54661 cgattttatc agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct
    54721 aaagaacggg caatttttcc gatattacca gccccctgaa cgagaatcag gtcgttaccg
    54781 gttaataccg gtgccagcat ctcggctacc tgcgcagggt ccggcaccag aatgggatca
    54841 attttcccac gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc
    54901 gcttcgccag ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg
    54961 gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc
    55021 aggtttttat ccggccagcc tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg
    55081 tggtggccgt agtcatcgac cagcattgcc gtaccgcttt taccattcac tggctccagc
    55141 gggaattcac cgaggaaatc aaaacggcgc ccagtcccct ggaagctctc cagcgcacgt
    55201 aaaatagcct cgtcatcaat tccctcttcc gtagcaaccg caaccgcagc tgcggcgttc
    55261 agcgcgttat gacgacctgg cgcattcagg gtgacgcgca tcggctcttt gtcctggcgc
    55321 agcagcgtaa agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca
    55381 tcttcgctga agccgtaagt cgtggtctga cgtcccactc gcggtaacaa ttcgcggatc
    55441 accggatcat caacacacat caccgcacga ccgtaaaacg gcaggttgtg cagaaaatta
    55501 ataaaagtct gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg
    55561 atattggtga caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct
    55621 gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat gaacccccgc cgcttttacc
    55681 agcccgccgt taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggaaaccatc
    55741 gcggtggttg tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt
    55801 aactcagcca gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca
    55861 atttccgggt tatcggcaga aatggcgctg gaaacaacga ccacgctggc atcacgtacg
    55921 ttttccgggc gatggttgaa ataaatcgtc gctcccagat tcattaactg ctgcgtgacc
    55981 ggatttggcg ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca
    56041 ataccgccca taccggcacc accgatgccg acaaaatgta tgtgccgaac gcgacgcatt
    56101 tcgggcacga tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac
    56161 ttcttaaatt catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca
    56221 cttcatttgc cactcgctcg gtggcatccg gaatggatgc agcgcgggcg cgttctgcca
    56281 tggttaataa ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa
    56341 gctgtggctg ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt
    56401 attgctggcg gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga
    56461 tttcactcac cgttaaagca ccggagcggc aaacgacgac atccgcccac gcatacgccg
    56521 ccgccatatc atcaataaat tccgtcactt tatgctgcgg ttgccccgct tcggcatacg
    56581 cctgttcaac ggattgttgc gaacctttac cgctctgatg ccagatagtg actgaatcac
    56641 ccagtttcgc agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac
    56701 ccactaccag cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggca
    56761 gcgccagtac atcggtacgc accgggttac ccaccacttc cgcattaggg aaagcgcctg
    56821 gaaacgcctg catcactttg gtggcaatct tcgccagcca tttattggtt aagcccgcaa
    56881 taccgttttg ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccacctg
    56941 gacctgatac atagccgccc ataccgagta ccacgtcagg tttgtacgct ttcataatcg
    57001 cccgcgcctg acgccaggcg ttgaagatac gcagcggcgc agctatcagt gcttttatac
    57061 cttttccacg cagaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta
    57121 agtccgcttc catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta
    57181 gatggtgcgc aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca
    57241 ttaatcgctt tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag
    57301 acgcgtttca taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct
    57361 cgaaccaccg taactgatca gcggcaatgt cagacctttg gtcggtaaca tccccgccgc
    57421 cgcgcctacg ttaaccagcg cctggaagct aaaccagatg ccaatagaac aggcgagaaa
    57481 accggaaaaa cggtggtcaa tttctaacgc tttgcggcca atcgacatcg cgcgaaaagc
    57541 gacgaagaat accattaaaa gtgccagcac cacaccgaca taccccagtt cttcgccgat
    57601 aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgagtt
    57661 acctaaacct tgtccccaaa gttcgccgcg accaaacgcc atcagcgatt gcgttaactg
    57721 atagccgctg ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg
    57781 gtacggttcg gcgagtatca gcaacacaac cgctgaaatg cccataccga taatggcaat
    57841 gaactgccac aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac
    57901 caccaccgta ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc
    57961 catcggtttc aggaagccgc gcaggttatt acgtacttcg tcgcctttac gcaccagata
    58021 gttggcgata taacaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa
    58081 accgagatcg atccaacgcg atgccccttt aaccgagcta cccactacca gcacgatcat
    58141 cagcaggatg atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg
    58201 cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc agatagacgc catcacgctt
    58261 cgcgaagaag aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac
    58321 catgataaag ccaatcgccg caaggccgaa ggtcagccac agtaaggtgc gatcgtacat
    58381 gatcaggctg tcggtatctt tttcccgcga gcccatcacc cagcctttta gcgccgtgga
    58441 gatccagacc aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa
    58501 acgcatcaac ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc
    58561 ttgaactgat caaggctggc acaggccggg gagagcagaa ccatatcgcc cggctgaaca
    58621 cgcggagcca gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc cacttccggg
    58681 cgtagcgccg ccagctgcgc accgtcacga ccaaaacagt acaaacgcac gttgtcgcca
    58741 ttcagatagc gcgccagtgg actgaagtcc gccgatttac catcgccgcc caacaacaaa
    58801 tgcagcgtgc cgtctacctg caagccattc aacgccgctt ccgtactgcc gacgttagtg
    58861 gctttcgaat cgttaatcca gcgtacgcca ttgtgctcca gcaccacttc aaagcgatgc
    58921 ggcagaccgg tgaaggtggt taacgctttc aggctgctgg cgcgcggcaa accagctgcg
    58981 tctgccagtg ccagtgctgc cagcgcattg gtgtagttat gctgcccgga aagtttcatc
    59041 tctttcacgt tcagcacttt ctcgcctttc actcgcagcc aggtttctcc ctgctgatga
    59101 ttcaggtgat agtcacccat gttgacgcca aagctgacgc agcgttcatc agcaccgcga
    59161 atcggcattg ttaaggcatc atcagcattg acgacacaaa ctttcgcatt ttcgtaaata
    59221 cgcaattttg ccgcacggta ttgctgcaaa ccaaacggat agcggtccat atgatcttca
    59281 gtcacgttca gaatggtcgc cgccaccgcc tgtaagctgg aagtggtttc cagctggaag
    59341 ctcgacagtt ccagcacgta cagttcgcac tcagcatcaa gcagcatcaa cgctggcagg
    59401 ccaatattgc cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc
    59461 gtggtgaccg tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg
    59521 cggcagaaca gctcgatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag
    59581 gatggatgcg ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat
    59641 tcatcattca gactgcccgt gtggcgttct acggcttcgg gtaatttatc caggccaggc
    59701 ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg
    59761 caggaaagcc cagtgaggcc caggccgata atgacgacat ttttaccctg ataatcagcc
    59821 atgattaacg taccttcagc gttgccagac caatcagaac cagcatcagc gaaataatcc
    59881 agaaacgcac aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg
    59941 gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag
    60001 aaagcgtttc taccacgaac acgcccccca taatcaccag caggaattcc tgacgtagca
    60061 gtacggcgat aatgcctaac gcaccaccta acgccagcga acctacatcg cccataaaga
    60121 cctgcgccgg ataggtgtta aaccacagga agcccagtcc tgccccgact atcgccgtgc
    60181 agacaatgac cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt
    60241 tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca
    60301 taattgccag gccgtcgaga ccatcggtca ggtttaccgc gttgccagta cccacaatga
    60361 cgaagtaagc cagcagaatg tagaacagcc ccagctgcgg catcacatct ttaaagaatg
    60421 ggaccaccag ctgcgttgcg ggcgtgtctt tgccggcaag gtacagggcg aaggcgatac
    60481 ccagcgcaat gaccgacatc cagaaatact tccaacgagc gatcaaccct ttggtgtctt
    60541 tacgcaccac tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca
    60601 ccaacacgca ccagacgtac ggattggacg ggtaagccca cagcagtacg gagatcacaa
    60661 tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt
    60721 caggaccgtc gttacgcacc acctgaccaa aggaaagttt ttgcaaatga gcaatcatac
    60781 gcgggcccat ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg
    60841 tcagatagga aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa
    60901 ctaacatgtc ccattctcct gtaaagcgcg tactacctct tccatggcgg cactacgtga
    60961 acccttaact aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat
    61021 taacgcagtt ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct
    61081 ttgtttaccc acgcttaaca cgcggtcaat accagcagct tttgccgcct cgcccacctg
    61141 tacatggcag gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg
    61201 gtagcccggc atttcagcca gtacctgaac agcggcggtc attgaaccga cattggcgtt
    61261 gtaggagtcg tcgagcagca actggttttc tgccagttgg atggggaaca gacggcctgg
    61321 aacagctttc agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat
    61381 ggagagcgca gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag
    61441 aacatcgacg ctaccggttg gggtttgtag ggtaaattcc gtaccgtgcg aggtcacatg
    61501 gatattggtg gcggtgaaat cgctgttggc ggcattgggt gagaaacgcc acactttgcg
    61561 tgagccaatt acgctctgcc agttcagcca gtcgttgttg tcggcgttca taatggcgat
    61621 accgttttcc ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga
    61681 gccaaaacct tccagatgcg ccgctgccag gttgttgacc agcgcagctt ccgggcgagt
    61741 cagactcaca gtccaggcta tttcgccctg atggttcgcg ccaagttcaa taactgcgta
    61801 atcgtattcc ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt
    61861 gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac
    61921 ggaggttttg ccggaggacc ccgtcagagc aaccacgcgc gccggaactt gctggcgaac
    61981 ccatgcagcc agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc
    62041 gatgtccagc ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc
    62101 aaaatcgtgg gcatcaaaac gttcgccttt cagggcaaca aacaggcagc ccggcgtcag
    62161 ttttcgggta tcagtggtta cagcatcaag ggtgatatct gcaccttgca gttcaccgtt
    62221 gagaatatcg gtaagttggc taagggttac gctaatcatg caatcacccc cagcagacgc
    62281 gccaccgtga cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcctca
    62341 tggcctttgc ccgcgaccag taccacatca ttctctttag cctgcataac ggcgcaagtc
    62401 accgcttcag cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga
    62461 atatcgttga tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc
    62521 acgtcagcaa actcttcggc aattgcgccc atcagtggac gcttaccttt atcgcgatcg
    62581 ccaccacagc caaagacgca ccacagcttg cccgcacagt gcaggcgcgc cgcctgtaag
    62641 gctttttcca gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc
    62701 gcagtgaaca cttccatacg tccgcaaacc ggttgcagac gcgcggcggt ttttaacaga
    62761 tcagccagcg ggtagccaag tgccagcagt gtcgccagcg cgagcagcag gttgctgacg
    62821 ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc cccaacttga actaaagcga
    62881 atggtcgcgc cgctgtcgtg atagttcact tcggtcgctt tcaaccagcg tccgtgacag
    62941 ttcggattga tatgatcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag
    63001 cggcggccca cttcatcgtc ggcgttaaca atcgcctgac cacaatgatg ttcagaataa
    63061 agcagccatt tcgcggcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg
    63121 cttaagttgg taaagaccga cgccgcaaat ttcaatgccg ccacacggtg ctgtaccagc
    63181 ccgtgggagg aaacttccat tgcgcaaaac gtcgcgccct gatccaccag ccccgccagc
    63241 tcatgctgaa catcgactgc cgaaccggtg gtattttctg tcgggatcac tttccccagc
    63301 agaccgttac caacggtgcc cattaccgcg ctggtttcgc caagcagttg gctccactgc
    63361 gccagcagct gggtagtcgt ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa
    63421 ttgtcagagg gttcatggta aaagcggccc gccagtgcag ataaacgctc gttgagctgg
    63481 ctgagataga tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcacct
    63541 ttcgcctctg caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc
    63601 ccgtccgcct gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg
    63661 tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg
    63721 tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt
    63781 atcgcccgtt gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat
    63841 ggcaccaaag accggcgctg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt
    63901 gataacaaca accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc
    63961 aatatattta ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat
    64021 ggcgatacga tagcctttaa tcgccgcctt cacgccgccg ccgcctggta gcgccacgct
    64081 ttccatcata tgcaccacag tgcggacaat ggattccggg aagacacgtt caccgggaac
    64141 cggggggtca actttggtaa tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc
    64201 gtagactcgc gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc
    64261 cctctctatg tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa
    64321 ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acgctgagga
    64381 cggcatcgct aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt
    64441 cagggttaat tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa ttcgataagg
    64501 aatggtattg agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg tcattaccac
    64561 catcggttta accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc
    64621 ctctttcggc gtgccgctca gattgttagg gttgtatgac gggctgttag ccatcgccag
    64681 cacttcaccg gtgttgacat ccaccagcac ggcgctacca gattcagcct tgttaaaggc
    64741 caccgcgttg ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc
    64801 caggttgtgc gctgcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg
    64861 gtctttacgc acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc
    64921 aacgccctca atcccttgac tatcgacgtt agtaaagccg atgaggtgag cagtcacttc
    64981 gccggacgga tagtaacggc gagactcttc acgcagatga atccccggca gtttcagttt
    65041 tttgatgtag tccgccatgt cagggttcac ctgacgcgcc agataaataa agcgcccttt
    65101 cgggttggcg ttaatgcggg ctgaaagctg atccagcgga atattgagcg cgttagccag
    65161 cgccttccag cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc
    65221 ccaaatcgct tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat
    65281 gccgcgggag gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag
    65341 catatccggg gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag
    65401 gagaatacag ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg
    65461 acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg
    65521 cactacgata ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat
    65581 cctttccacc cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc
    65641 aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt
    65701 atggtgcgcc gtggttacca cagtcaccgc cgtcaaaata atgcaaatga acaggcagag
    65761 tggcagcttc ccaaatcgca aaagatcgtc accgataaca gcaggcaatg catggcgctc
    65821 gtggcttccc atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgca
    65881 ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac gagggttctc agccacctct
    65941 tcttcgcccg gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc
    66001 tgctcttcag tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata
    66061 aaacgtttca caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgcccaccc
    66121 ggggccagca cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt
    66181 acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc
    66241 ggcgttgcag cagccacgac ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg
    66301 ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac gctcttcacc ataggttttc
    66361 aatacccagg cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca
    66421 cgggttgggt ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgctca
    66481 gcatcatcaa gttgcggtga agagacgcca agatcgagga gaatgccgtc gatcttgccg
    66541 ataagatcgc gctcggcaac gtattcgccc agcgcggaga aaggtccgtg gatgatggag
    66601 aagcgcggat catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc
    66661 agcaaacgcc cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca
    66721 aaagtcccat caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc
    66781 agcagcaccg tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg
    66841 ctccgataag tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg
    66901 ccaggttgtt tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt
    66961 tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat
    67021 ctgacattcg ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat
    67081 gctcgacaga cgcgataatt tttgctcgat aatttcccat tcaggtaggg ggtaaagcag
    67141 caggcacggg tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag
    67201 ctgttcccgg taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt
    67261 tgctccccgg aacatgccag cctcacccct tattcccact ttgccccaca atttcccact
    67321 gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt
    67381 aaaaaacccg atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgcag
    67441 caaattaacg ttatgaatag ctgtaagaaa aaatccctca tcgtcatcac cgttttaaaa
    67501 tgactcacta ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta
    67561 ggacatatct tccccggtta acagtccctg ttgcgtctgt gtggcgcttg atgccagcgg
    67621 gtttgcgcaa aagcaaagaa aagtgcttgt taaatcggca attcatctct gcatgtaaca
    67681 aaataatgca aaaacgcgct gtcgatatca cttaattatt gtgaatttca gctggtgagt
    67741 taattaacgg gcgggtttaa aagaacttat caataatagt caggaatagt cttatttact
    67801 ttaaggatat tgatgtccat tcccgtactc tacgcgccag agggaaattc acctggcgcg
    67861 tatttttgtt cgcggcttag ctacggctga gcacaccgcg gcgatagaga ttacgtttaa
    67921 tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag gcttgccagg acaatctcca
    67981 gcacacgctc tgcgacatcg cggtgacgtt gagccactgc cagcaccggg cactgtaaga
    68041 agtcgagcag ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttaccat
    68101 cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt gtgaacagcg
    68161 cctgcggcat cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct
    68221 catagctgtt ggcatacagg aaatgcactt cgcgcggatc atctttccag gcagtacgga
    68281 aaccttgttc acgcaggaag ctgacagaaa gctccggcag cgcgccaaga taaagcaccg
    68341 tctcggcggg aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac
    68401 caaccacgct ggtgaaatgt tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg
    68461 ggtcgttagc ccagcgttga taaaaaggat gctcaggtgg caacgacgtc gaaacaataa
    68521 tggcatcaac ctgacgctgt aaaaggtgct caatgcagcg catttcgttg tctggctgat
    68581 cttctgagca ggcaatcagc agttgataac cccgttgccg cgcctggcgc tcaagatagt
    68641 tagcgatgcg ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacgtg
    68701 tgcgtccagc acgaagccca gctgccacgg cgttcgggtg gtaattgtgc tcacgcacca
    68761 cagccatgac tttttcaacg gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa
    68821 taacatagct tgcagtggtc cgcgacactc ccgccagccg agcgatttca tccagtttca
    68881 caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg ggttaaattc
    68941 attaacatct aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg
    69001 atttcgcaaa aaaagcccaa cgtgacatgt tgggctgcaa attgcgcact gagatcattc
    69061 tcaacgcatt attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac
    69121 aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc
    69181 taattgaacg gtataaagcg agggggtgac atcgataatt tgcccacgga atatttccgt
    69241 attacgtttc acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc
    69301 ccgctcaaca tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg
    69361 taattgcttt tcgatctgct caagcacttt ttcatcgccc acggtctgga tggtcatacg
    69421 cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agccacgctg
    69481 ggaaaaaagg ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa
    69541 tatccggcgc ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc
    69601 ccgcgaatct gcatcgggta gacgtgctca ctgccatcga cggtaacatc aacaaacacc
    69661 aggcgattat tgcacacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga
    69721 tgagagatct ggatcccgac gtgaccatag gcttccgcca gacggacgaa atcgggtagt
    69781 gattgcatat aagattgtga atgacggccg gaatagatca tgtcctgcca ctgtttcacc
    69841 atccccagat agcggttatt gagattcacc accagtaccg gcaactcgta ttgcaacgcg
    69901 gtagacagtt cctggatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt
    69961 tcttctggca acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg
    70021 aggccaccgg aattgatcca gcgacgcggt ttgtcgaatg gataataaag tgcagcaaac
    70081 atctggtgct gcccgacatc ggacgtcacg taagcgtctc ccttcgtcaa ccgccaaaga
    70141 gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga
    70201 cgagcgcgcc actgttcaat ttgctgccac cagtcgcgga tctcatccag tggttgatgg
    70261 gcggattctt gcgacaagag ttcaagcatt tgttcgagga cctggcgagc atcccccaca
    70321 atcgggatat ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga
    70381 acagtggcat ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc
    70441 ccgacggcga aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc
    70501 attcccagca tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat
    70561 gaggaaacaa cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc
    70621 gccatgattg ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc
    70681 agagcgcgct taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg
    70741 accgactcag gccagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa
    70801 tcaacgacca ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc
    70861 tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc
    70921 cccaccatgt cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg
    70981 gaaaggacaa ctaatggaat ggaatccata taagcggtgg cgatgccggt aatcgcattg
    71041 gtcgcccctg gacccgacgt taccagcacg acgccgactt ccccggtcgc gcgcgccaga
    71101 ccatcggcca tatgcactgc cgcctgctca tgacgaacta aaacatgatc gataccaccc
    71161 acggtatgca atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt
    71221 ttaacgccct gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg
    71281 gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt
    71341 aagcaattgg cagccagaga cataaacgca gggtaataag aataaacagg aggaaaggtg
    71401 aaaagaaacg ataaaatcct ccattacgaa ggattaaatt gataataaat cggctgaatc
    71461 ccacaactta cagcaatttc tctatttttt taattaatcc taaaacagag agcccacaca
    71521 ttcagaataa aattctacat tcaataaaaa atacagaata aaccagacat tcatgcctga
    71581 cctattctgc attcagttag cgtttgcaaa ttgagactaa ttgctcttcc atccactgat
    71641 ggcctttatc gcgcccggca gcttcatgcc aggagagata acaggttctg ctattttgtt
    71701 ttaacggcag cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac
    71761 gcggcgcaat agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca
    71821 tgccctgata cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg
    71881 acgcgaaacg atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta
    71941 acgggccctt aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata
    72001 atggcacgct ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt
    72061 cctgataacg cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta
    72121 tatttggcgc aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg
    72181 ggctgcaaac acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag
    72241 gcaattcatt ttgtactagt tgcaatgcct gacgaactga gccaaaaagt tgaaatgcgc
    72301 gagcagtcgg ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct
    72361 tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat acccagaaca tgagcggcac
    72421 gggtaatgtt ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca
    72481 tgcgtagctg tggcttgctt aactccgccg tctctgaatg atctgtttgt acctctggca
    72541 tacttaactc cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc
    72601 ctgaagaata tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg
    72661 aaaacataaa gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt
    72721 accacagcat aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc
    72781 gaatactcaa tcatctacaa aatggattaa atgtgcttat ttaata
//
LOCUS       DAZUFB010000010        69578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZUFB010000010 DAZUFB010000000
VERSION     DAZUFB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.010"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..1039)
                     /gene="abgR"
                     /locus_tag="ACHY3M_000794"
     CDS             complement(131..1039)
                     /gene="abgR"
                     /locus_tag="ACHY3M_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4315765.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTLLTASLITQFRRECGYLQS"
     gene            1215..2525
                     /gene="abgA"
                     /locus_tag="ACHY3M_000795"
     CDS             1215..2525
                     /gene="abgA"
                     /locus_tag="ACHY3M_000795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFS4315766.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            2525..3970
                     /gene="abgB"
                     /locus_tag="ACHY3M_000796"
     CDS             2525..3970
                     /gene="abgB"
                     /locus_tag="ACHY3M_000796"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFS4315767.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTCNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            4007..5533
                     /gene="abgT"
                     /locus_tag="ACHY3M_000797"
     CDS             4007..5533
                     /gene="abgT"
                     /locus_tag="ACHY3M_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFS4315768.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            5544..6059
                     /gene="ogt"
                     /locus_tag="ACHY3M_000798"
     CDS             5544..6059
                     /gene="ogt"
                     /locus_tag="ACHY3M_000798"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4315769.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            6254..7006
                     /gene="fnr"
                     /locus_tag="ACHY3M_000799"
     CDS             6254..7006
                     /gene="fnr"
                     /locus_tag="ACHY3M_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFS4315770.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            7158..8108
                     /gene="uspE"
                     /locus_tag="ACHY3M_000800"
     CDS             7158..8108
                     /gene="uspE"
                     /locus_tag="ACHY3M_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFS4315771.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHELAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8158..8415)
                     /gene="ynaJ"
                     /locus_tag="ACHY3M_000801"
     CDS             complement(8158..8415)
                     /gene="ynaJ"
                     /locus_tag="ACHY3M_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFS4315772.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            8659..9690
                     /gene="ynaI"
                     /locus_tag="ACHY3M_000802"
     CDS             8659..9690
                     /gene="ynaI"
                     /locus_tag="ACHY3M_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFS4315773.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(9741..11354)
                     /gene="mppA"
                     /locus_tag="ACHY3M_000803"
     CDS             complement(9741..11354)
                     /gene="mppA"
                     /locus_tag="ACHY3M_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFS4315774.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYPYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(11691..12590)
                     /gene="pgrR"
                     /locus_tag="ACHY3M_000804"
     CDS             complement(11691..12590)
                     /gene="pgrR"
                     /locus_tag="ACHY3M_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFS4315775.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            12728..13648
                     /locus_tag="ACHY3M_000805"
     CDS             12728..13648
                     /locus_tag="ACHY3M_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4315776.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            13648..14578
                     /locus_tag="ACHY3M_000806"
                     /pseudo
     CDS             13648..14578
                     /locus_tag="ACHY3M_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            14596..15234
                     /locus_tag="ACHY3M_000807"
     CDS             14596..15234
                     /locus_tag="ACHY3M_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4315777.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            15543..16271
                     /gene="mpaA"
                     /locus_tag="ACHY3M_000808"
     CDS             15543..16271
                     /gene="mpaA"
                     /locus_tag="ACHY3M_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFS4315778.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(16246..17211)
                     /gene="ycjG"
                     /locus_tag="ACHY3M_000809"
     CDS             complement(16246..17211)
                     /gene="ycjG"
                     /locus_tag="ACHY3M_000809"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFS4315779.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     IVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            17330..17836
                     /gene="tpx"
                     /locus_tag="ACHY3M_000810"
     CDS             17330..17836
                     /gene="tpx"
                     /locus_tag="ACHY3M_000810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFS4315780.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(17880..19421)
                     /gene="tyrR"
                     /locus_tag="ACHY3M_000811"
     CDS             complement(17880..19421)
                     /gene="tyrR"
                     /locus_tag="ACHY3M_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFS4315781.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(19569..20630)
                     /gene="ycjF"
                     /locus_tag="ACHY3M_000812"
     CDS             complement(19569..20630)
                     /gene="ycjF"
                     /locus_tag="ACHY3M_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFS4315782.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(20627..22024)
                     /gene="ycjX"
                     /locus_tag="ACHY3M_000813"
     CDS             complement(20627..22024)
                     /gene="ycjX"
                     /locus_tag="ACHY3M_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFS4315783.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            22179..23177
                     /gene="ycjW"
                     /locus_tag="ACHY3M_000814"
     CDS             22179..23177
                     /gene="ycjW"
                     /locus_tag="ACHY3M_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4315784.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIVMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(23288..24193)
                     /gene="ompG"
                     /locus_tag="ACHY3M_000815"
     CDS             complement(23288..24193)
                     /gene="ompG"
                     /locus_tag="ACHY3M_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFS4315785.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(24238..25320)
                     /locus_tag="ACHY3M_000816"
     CDS             complement(24238..25320)
                     /locus_tag="ACHY3M_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4315786.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGTSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(25334..25993)
                     /gene="pgmB"
                     /locus_tag="ACHY3M_000817"
     CDS             complement(25334..25993)
                     /gene="pgmB"
                     /locus_tag="ACHY3M_000817"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4315787.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(25990..28257)
                     /gene="ycjT"
                     /locus_tag="ACHY3M_000818"
                     /pseudo
     CDS             complement(25990..28257)
                     /gene="ycjT"
                     /locus_tag="ACHY3M_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            complement(28254..29309)
                     /gene="ycjS"
                     /locus_tag="ACHY3M_000819"
     CDS             complement(28254..29309)
                     /gene="ycjS"
                     /locus_tag="ACHY3M_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4315788.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(29319..30107)
                     /gene="ycjR"
                     /locus_tag="ACHY3M_000820"
     CDS             complement(29319..30107)
                     /gene="ycjR"
                     /locus_tag="ACHY3M_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4315789.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(30125..31177)
                     /gene="ycjQ"
                     /locus_tag="ACHY3M_000821"
     CDS             complement(30125..31177)
                     /gene="ycjQ"
                     /locus_tag="ACHY3M_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4315790.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(31208..32050)
                     /gene="ycjP"
                     /locus_tag="ACHY3M_000822"
     CDS             complement(31208..32050)
                     /gene="ycjP"
                     /locus_tag="ACHY3M_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4315791.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(32037..32918)
                     /gene="ycjO"
                     /locus_tag="ACHY3M_000823"
     CDS             complement(32037..32918)
                     /gene="ycjO"
                     /locus_tag="ACHY3M_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4315792.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(32939..34231)
                     /gene="ycjN"
                     /locus_tag="ACHY3M_000824"
     CDS             complement(32939..34231)
                     /gene="ycjN"
                     /locus_tag="ACHY3M_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4315793.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(34245..35924)
                     /gene="ycjM"
                     /locus_tag="ACHY3M_000825"
                     /pseudo
     CDS             complement(34245..35924)
                     /gene="ycjM"
                     /locus_tag="ACHY3M_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(36136..36450)
                     /gene="pspE"
                     /locus_tag="ACHY3M_000826"
     CDS             complement(36136..36450)
                     /gene="pspE"
                     /locus_tag="ACHY3M_000826"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFS4315794.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(36525..36746)
                     /gene="pspD"
                     /locus_tag="ACHY3M_000827"
     CDS             complement(36525..36746)
                     /gene="pspD"
                     /locus_tag="ACHY3M_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFS4315795.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(36755..37114)
                     /gene="pspC"
                     /locus_tag="ACHY3M_000828"
     CDS             complement(36755..37114)
                     /gene="pspC"
                     /locus_tag="ACHY3M_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFS4315796.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(37114..37338)
                     /gene="pspB"
                     /locus_tag="ACHY3M_000829"
     CDS             complement(37114..37338)
                     /gene="pspB"
                     /locus_tag="ACHY3M_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFS4315797.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(37392..38060)
                     /gene="pspA"
                     /locus_tag="ACHY3M_000830"
     CDS             complement(37392..38060)
                     /gene="pspA"
                     /locus_tag="ACHY3M_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFS4315798.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            38227..39204
                     /gene="pspF"
                     /locus_tag="ACHY3M_000831"
     CDS             38227..39204
                     /gene="pspF"
                     /locus_tag="ACHY3M_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFS4315799.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(39324..40589)
                     /gene="puuE"
                     /locus_tag="ACHY3M_000832"
     CDS             complement(39324..40589)
                     /gene="puuE"
                     /locus_tag="ACHY3M_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFS4315800.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDTAMKILQDALSD"
     gene            complement(40627..41907)
                     /gene="puuB"
                     /locus_tag="ACHY3M_000833"
     CDS             complement(40627..41907)
                     /gene="puuB"
                     /locus_tag="ACHY3M_000833"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFS4315801.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTDNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(41909..43396)
                     /gene="puuC"
                     /locus_tag="ACHY3M_000834"
     CDS             complement(41909..43396)
                     /gene="puuC"
                     /locus_tag="ACHY3M_000834"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFS4315802.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(43671..44228)
                     /gene="puuR"
                     /locus_tag="ACHY3M_000835"
     CDS             complement(43671..44228)
                     /gene="puuR"
                     /locus_tag="ACHY3M_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFS4315803.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(44255..45019)
                     /gene="puuD"
                     /locus_tag="ACHY3M_000836"
     CDS             complement(44255..45019)
                     /gene="puuD"
                     /locus_tag="ACHY3M_000836"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFS4315804.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            45231..46649
                     /gene="puuA"
                     /locus_tag="ACHY3M_000837"
     CDS             45231..46649
                     /gene="puuA"
                     /locus_tag="ACHY3M_000837"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFS4315805.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDHHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            46798..46962
                     /locus_tag="ACHY3M_000838"
     CDS             46798..46962
                     /locus_tag="ACHY3M_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315806.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            46952..48337
                     /gene="puuP"
                     /locus_tag="ACHY3M_000839"
     CDS             46952..48337
                     /gene="puuP"
                     /locus_tag="ACHY3M_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFS4315807.1"
                     /translation="MAINSPLNIAAQPGKTSLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            48471..48716
                     /gene="ymjA"
                     /locus_tag="ACHY3M_000840"
     CDS             48471..48716
                     /gene="ymjA"
                     /locus_tag="ACHY3M_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFS4315808.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            49029..50672
                     /gene="sapA"
                     /locus_tag="ACHY3M_000841"
     CDS             49029..50672
                     /gene="sapA"
                     /locus_tag="ACHY3M_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFS4315809.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYRKKQDEVKKP"
     gene            50669..51634
                     /gene="sapB"
                     /locus_tag="ACHY3M_000842"
     CDS             50669..51634
                     /gene="sapB"
                     /locus_tag="ACHY3M_000842"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFS4315810.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTQAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            51621..52511
                     /gene="sapC"
                     /locus_tag="ACHY3M_000843"
     CDS             51621..52511
                     /gene="sapC"
                     /locus_tag="ACHY3M_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFS4315811.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            52511..53503
                     /gene="sapD"
                     /locus_tag="ACHY3M_000844"
     CDS             52511..53503
                     /gene="sapD"
                     /locus_tag="ACHY3M_000844"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFS4315812.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            53505..54311
                     /gene="sapF"
                     /locus_tag="ACHY3M_000845"
     CDS             53505..54311
                     /gene="sapF"
                     /locus_tag="ACHY3M_000845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFS4315813.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            54379..54732
                     /gene="ycjD"
                     /locus_tag="ACHY3M_000846"
     CDS             54379..54732
                     /gene="ycjD"
                     /locus_tag="ACHY3M_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFS4315814.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            55100..55888
                     /gene="fabI"
                     /locus_tag="ACHY3M_000847"
     CDS             55100..55888
                     /gene="fabI"
                     /locus_tag="ACHY3M_000847"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4315815.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            56033..57160
                     /gene="yciW"
                     /locus_tag="ACHY3M_000848"
     CDS             56033..57160
                     /gene="yciW"
                     /locus_tag="ACHY3M_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFS4315816.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            57228..59162
                     /gene="rnb"
                     /locus_tag="ACHY3M_000849"
     CDS             57228..59162
                     /gene="rnb"
                     /locus_tag="ACHY3M_000849"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFS4315817.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(59240..59344)
                     /locus_tag="ACHY3M_000850"
     CDS             complement(59240..59344)
                     /locus_tag="ACHY3M_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315818.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            59398..61383
                     /gene="pdeR"
                     /locus_tag="ACHY3M_000851"
                     /pseudo
     CDS             59398..61383
                     /gene="pdeR"
                     /locus_tag="ACHY3M_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(61365..61493)
                     /gene="ymiD"
                     /locus_tag="ACHY3M_000852"
     CDS             complement(61365..61493)
                     /gene="ymiD"
                     /locus_tag="ACHY3M_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFS4315819.1"
                     /translation="MQKLPLKEKCLTATANYHPRIRYIMTGYSAKYIYSSTYARFR"
     gene            61531..61704
                     /gene="yciZ"
                     /locus_tag="ACHY3M_000853"
     CDS             61531..61704
                     /gene="yciZ"
                     /locus_tag="ACHY3M_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFS4315820.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            61794..62543
                     /gene="yciT"
                     /locus_tag="ACHY3M_000854"
     CDS             61794..62543
                     /gene="yciT"
                     /locus_tag="ACHY3M_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFS4315821.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSRIRASDLMHLEHSKLTVHVVDI"
     gene            62812..63030
                     /gene="osmB"
                     /locus_tag="ACHY3M_000855"
     CDS             62812..63030
                     /gene="osmB"
                     /locus_tag="ACHY3M_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFS4315822.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(63156..63482)
                     /gene="yciH"
                     /locus_tag="ACHY3M_000856"
     CDS             complement(63156..63482)
                     /gene="yciH"
                     /locus_tag="ACHY3M_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFS4315823.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(63482..64219)
                     /gene="pyrF"
                     /locus_tag="ACHY3M_000857"
     CDS             complement(63482..64219)
                     /gene="pyrF"
                     /locus_tag="ACHY3M_000857"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4315824.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(64411..65580)
                     /gene="lapB"
                     /locus_tag="ACHY3M_000858"
     CDS             complement(64411..65580)
                     /gene="lapB"
                     /locus_tag="ACHY3M_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFS4315825.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(65587..65895)
                     /gene="lapA"
                     /locus_tag="ACHY3M_000859"
     CDS             complement(65587..65895)
                     /gene="lapA"
                     /locus_tag="ACHY3M_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFS4315826.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(66044..66808)
                     /gene="pgpB"
                     /locus_tag="ACHY3M_000860"
     CDS             complement(66044..66808)
                     /gene="pgpB"
                     /locus_tag="ACHY3M_000860"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFS4315827.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPEEENREIAQREQES"
     gene            66978..67568
                     /gene="ribA"
                     /locus_tag="ACHY3M_000861"
     CDS             66978..67568
                     /gene="ribA"
                     /locus_tag="ACHY3M_000861"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFS4315828.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(67632..>69578)
                     /gene="acnA"
                     /locus_tag="ACHY3M_000862"
     CDS             complement(67632..>69578)
                     /gene="acnA"
                     /locus_tag="ACHY3M_000862"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFS4315829.1"
                     /translation="MLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEF
                     YGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAK
                     AQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNA
                     THKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKR
                     QPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAI
                     KKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIGHDR
                     KGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQE
                     DSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYL
                     QGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIY
                     DAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGM
                     GILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRI
                     DTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        16820 a        18150 c        17233 g        17375 t
ORIGIN      
        1 taaaggttaa aaccgttata acacagtcac cggcgcagag gagacaatgc cggacttaag
       61 acgcggatgc actgctgtgt gtactgtaga gtctggcgga tgtcgacaga ctctattttt
      121 ttatgcagtt ttaactttgc agatagccgc attctcgccg gaattgcgtg attaatgacg
      181 cggtcagtag tgtctggcga ctatcacgcc gctgaatcaa ataataggcc gctttcggta
      241 aaatttcgct aaccggcaac atcaccagcc cctgtccgtg caagggatcg cagcccattt
      301 cttcaggcag tatgctgaga aaatcgcttt ttgccaccag actgatacag gctgagaacg
      361 tctcgcagac tacaccgacc tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact
      421 gtttgtagta actgccgtgt ggcgtcggca ttgtccagct gtaatccagt aactgtttga
      481 tcgaacgggc accaatggcg gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca
      541 gtaatttctc aaaagtaaat tcgtggtcgt acggtccctg ataataggta ttgatggtga
      601 aatccaattc tccctgacgc aattcattaa tcatcgacac cagttgccct tccataatgc
      661 gtacttttac ctgcggatgc tgctgatgaa aacgagatat gacagctggc atcagactgc
      721 gggaaatact ggcccccatg ccgatattaa tctgccctgc cagttgccct tgtcgttggc
      781 gaatttcctc ttgcgctgcg cgcagctctt caagaataag actggcgtgc tgataaaaac
      841 tttcaccggc atcagttaac gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta
      901 atccttcttc cagctcctga atagatttac tcagtgccgg ttgcgacata ttcaacattc
      961 ggctcgctcc gcgaatgctg ccctgacgag ccacttcaac aaaagcccga atttgatgaa
     1021 tttttacctg aaaagccatg acgccaccga taaccgttat ttatcagacc aaagaaactg
     1081 gcatctactt taatgcagat gattgtgtca gggtaattta tgaacggtta aaactgtgaa
     1141 aaatcagtta gtgataagta aaaactatcg ctacgtgaac cgggtcacac tttttactga
     1201 tgacgggaaa ggttatggag tcgttgaatc aatttgttaa ttcgcttgcc ccaaaattat
     1261 cgcactggcg acgtgatttt catcactatg cagagtctgg ctgggtggaa ttccgcactg
     1321 ccacccttgt tgcggaagaa ttgcagcagc tcggctattc actggcgctg ggccgcgaag
     1381 tagttaatga aagtagccgg atgggactac ctgatgaact cactctacaa cgcgaattcg
     1441 agcgcgctcg tcaacagggt gcgctagcac aatggattgc ggtttttgaa ggtggtttca
     1501 cgggtatcgt cgccaccctg gataccggtc gccccggtcc ggttatggct ttccgtgtcg
     1561 atatggacgc gctggatctc agtgaagagc aggatgtcag ccatcgcccc taccgcgacg
     1621 gttttgcgtc atgtaacgcc ggaatgatgc atgcctgtgg tcatgatggg cataccgcca
     1681 ttgggcttgg gctggcgcat acccttaaac aattcgagtc cggactacat ggcgtcatca
     1741 aactgatttt tcagcctgca gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag
     1801 gtgtcgtaga tgatgttgat tattttactg ccgtgcacat tggcactggc gtacctgcgg
     1861 gcaccgtggt gtgcggcagt gataatttta tggcaaccac caaatttgac gcgcacttca
     1921 ccgggaccgc cgctcacgca ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg
     1981 cagcacaagc aactcttgca ctgcatgcaa tcgccccgca cagcgaagga gcttccagag
     2041 taaacgtggg cgttatgcag gcaggaagcg gccgtaacgt tgttcctgcc tcggcgttgc
     2101 tgaaagtgga aacacgcggg gccagcgacg tcattaatca atatgttttt gaccgtgcac
     2161 aacaagcgat tcagggcgca gcaaccatgt atggtgtcgg cgttgaaact cgcctgatgg
     2221 gtgcagctac cgccagttct ccttcgccgc aatgggtcgc atggttgcaa agtcaggcgg
     2281 ctcaggtcgc gggggtcaat caggccattg aacgtgttga agcgcctgcg ggttccgaag
     2341 atgccacatt aatgatggcc cgcgtgcagc aacatcaagg gcaagcctcc tacgtggtgt
     2401 ttggcacaca gctggcggca ggtcatcaca acgaaaaatt cgattttgac gagcaggttc
     2461 tcgctattgc cgtcgaaacg ctggcgcgca ccgcgctcaa ttttccctgg acgcgaggta
     2521 tctgatgcag gaaatctatc gttttatcga cgatgcgatt gaagccgatc gccaacgtta
     2581 taccgatatt gccgatcaaa tctgggatca tccagaaaca cgttttgaag agttctggtc
     2641 agcggagcat ctggcttcgg cgctggaatc tgcaagcttc accgttacct gcaacgtagg
     2701 caatatccca aatgcattta ttgcttcgtt tggtcaaggc aaaccggtta tcgccctgct
     2761 gggagaatat gacgccctgg caggtttaag tcagcaagct ggttgcgcgc aacctacatc
     2821 cgcgacgccc ggtgaaaatg gtcacggttg cggacacaat ttgctgggaa ccgccgcctt
     2881 tgccgctgca atagccgtca agaaatggct ggaacaatat gggcaaggcg gcacagtgcg
     2941 cttttatggt tgtcctggcg aagaaggcgg ctcgggtaaa acgttcatgg tccgcgaggg
     3001 ggtatttgat gatgtggatg cggcactcac ctggcacccg gaagcctttg ccggcatgtt
     3061 caatacccgc acgctggcaa acattcaggc atcatggtgc tttaaaggga tcgcagcaca
     3121 tgccgcgaat tcccctcatt tgggacgcag cgcccttgat gccgtaacgt tgatgaccac
     3181 tggcaccaac ttcctcaacg aacatattat tgaaaaagcg cgcgtacact atgccatcac
     3241 aaatagcggc gggatctcgc ccaacgtggt ccaggcgcag gcagaagtgc tttatcttat
     3301 ccgcgccccc gaaatgaccg acgtgcagca tatttatgat cgggtcgcca aaatcgccga
     3361 aggtgcggca ttgatgaccg aaaccacggt tgaatgccgc ttcgacaaag cctgttccag
     3421 ttatctcccg aatcgcacct tagaaaatgc catgtaccat gccctatccc attttggtac
     3481 cccggaatgg aactccgaag aactggcttt tgcgaaacaa attcaggcta cgctcacccc
     3541 caacgatcgg caaaacagtc tgaataatat cgccgcaacc ggtggcgaaa acggcaaggc
     3601 ttttgcacta cgtcatcgtg aaacggtact ggcgaatgag gtcgctccat atgccgccac
     3661 cgataacgtg cttgcggcat cgactgatgt cggcgacgtc agttggaaac tgcctgttgc
     3721 ccagtgtttc agcccctgtt ttgccgtcgg tacaccgcta catacgtggc aactggttag
     3781 ccaggggcga acatctattg ctcataaagg aatgctgctg gcggcgaaaa ctatggcagc
     3841 aaccacagtc aatctcttca ttgattcagg gctattgcaa gaatgccaac aagagcatca
     3901 gcaagttacg gacacgcaac cgtatcactg ccctatcccg aaaaacgtga caccgtcacc
     3961 tttaaaataa caacaacaac gcaaacacaa caaccgagga atgcccatga gtatgtcatc
     4021 cataccgtcg tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa ttggtaacaa
     4081 ggttccccat ccttttttgc tctttatcta tttgataatc gtactcatgg tgacgacggc
     4141 aattttgtca gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt
     4201 gaaaaacctg ctcagtgtgg aaggattaca ctggttttta cccaatgtga ttaaaaactt
     4261 tagcggtttt gctccacttg gagcgatcct ggcgctggtt ttaggtgccg gcctagcgga
     4321 gcgcgtcggc ttactgccag cgctaatggt taaaatggca tcgcatgtta atgcccgcta
     4381 cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg atgcggcgtt
     4441 agtgatcatg ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc atccagttgc
     4501 aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt tactgattgt
     4561 cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcggctgcgt tcaatccgca
     4621 aatgcacgtc agtgtaattg ataactggta ttttatggcc agctccgtag tcgtgctgac
     4681 gattgttggc ggcttgataa ccgacaaaat catcgagcca cggttaggtc aatggcaggg
     4741 aaacagcgat gagaaactgc agacattgac cgaaagtcag cgttttggtt tacgcatagc
     4801 aggtgtcgta tcgctacttt ttattgctgc gatcgcgctg atggtgatcc cggaaaacgg
     4861 gatattgcgc gatccgatta atcacaccgt gatgccatca ccctttatta aaggtatcgt
     4921 gccactaatc attctttttt tctttgtggt ttcgctggct tatggcatcg ctacccgcac
     4981 aattcgacgt caggcggatt taccgcattt aatgattgaa ccgatgaaag agatggcggg
     5041 atttatcgtg atggtttttc ccctcgccca atttgtcgcc atgtttaact ggagcaacat
     5101 ggggaaattc atcgccgtgg ggctgaccga tatacttgaa agttcagggc ttagcggcat
     5161 cccggcgttt gtcggtctgg cgttgctttc ctctttctta tgcatgttta ttgccagcgg
     5221 ttccgcaatc tggtcgattc tggcccccat tttcgtacca atgtttatgc tacttggctt
     5281 tcacccggca tttgcgcaaa tcctctttcg tattgccgac tcatccgtat tgcctttagc
     5341 gccggtatct ccttttgttc cactgtttct tggattcctg caacgctaca aaccagacgc
     5401 gaaactgggt acttactatt cgttagtctt gccctatccg cttatctttt tggtggtatg
     5461 gctgctgatg ttgctggcgt ggtatcttgt tggtctgccg ataggtccgg ggatttaccc
     5521 acgtttgtct taagagagaa cggatgctga gattacttga agaaaaaatt gccacgccac
     5581 tgggtccact gtgggtgatg tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag
     5641 agtacagcga acgcatggtg cagctgctgg acatccatta tcgtaaagaa ggctatgagc
     5701 gcatttctgc caccaaccca ggcggtttaa gcgacaagct tcgtgattat tttgccggta
     5761 atcttagcat tattgatacg cttcccactg ccacaggggg gacgccattt cagcgcgaag
     5821 tctggaaaac tctacgcact atcccctgcg ggcaggtaat gcattacggc caactggctg
     5881 aacaattggg ccgccctggc gcggcgcgtg ccgtaggtgc ggcaaatgga tcgaatccca
     5941 tcagcatcgt cgtaccttgc catcgggtta ttggtcgaaa cggcaccatg accggatatg
     6001 caggcggagt tcagcgaaaa gagtggttat tgcgccacga aggttatctt ttgctgtaaa
     6061 cattaaacaa tttgtgccag cttgttcaca cttttatgta aagttaccct taacaactta
     6121 agggttttca aatagataga catatattta catctaatat cggaattctc tgctgttaag
     6181 gtttgcttag acttacttgc tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg
     6241 cttgagcaga cctatgatcc cggaaaagcg aattatacgg cgcattcagt ctggcggttg
     6301 tgctatccat tgccaggatt gcagcatcag ccagctttgc atcccgttca cactcaacga
     6361 acatgagctt gatcagcttg ataatatcat tgagcggaag aagcctattc agaaaggcca
     6421 gacgctgttt aaggctggtg atgaacttaa atcgctttat gccatccgct ccggtacgat
     6481 taaaagttat accatcactg agcaaggcga cgagcaaatc actggtttcc atttagctgg
     6541 cgacctggtg ggatttgacg ccatcggcag cggccatcac ccgagcttcg cgcaggcgct
     6601 ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt ccggtaaaat
     6661 gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga
     6721 catgatcttg ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa
     6781 cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt gaattccgcc tgacgatgac
     6841 tcgtggcgat atcggtaact atctgggcct gacggtagaa accatcagcc gtctgctggg
     6901 tcgcttccag aaaagcggta tgctggcagt caaaggtaaa tacatcacca tcgaaaataa
     6961 cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt gcctgatttt tccgcataac
     7021 tcactatcct tctgtcatat cattaaattt ttctgattta ttgatctggc agaaggttca
     7081 tcactgtttc attcaccaga tatgggttaa tcttttaatt acaaactgcg ttgacagttg
     7141 ttgtaaggag accctgtatg gctatgtatc agaacatgct cgttgttatc gatcctaacc
     7201 aggacgacca accagcattg cggcgagctg tttatttaca tcaacggatt ggtggcaaaa
     7261 ttaaagcctt tttgccgatc tatgacttct catacgaaat gaccaccctg ctctccccgg
     7321 acgaacgtac cgctatgcgt cagggcgtca tcagccagcg tacagcctgg atccacgagc
     7381 tggcaaaata ttatctcaat gctggcgttc ccattgaaat taaagtggtc tggcataacc
     7441 gtcccttcga agccatcatt caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa
     7501 tggcgcacca acatgaccgt ctggaagcgg tgatttttac gccaacggac tggcatctgt
     7561 tacgcaaatg cccaagcccg gtgtggatgg tgaaagacca gccgtggccg gaaggaggca
     7621 aggcgctggt ggcggtgaat ctcgccagtg aagagccgta tcataatgcg ctcaatgaaa
     7681 aactggtcaa agagacgatc gaactggcag aacaagtcaa ccataccgaa gttcatctgg
     7741 ttggcgctta tccggtaacg ccaattaata tcgcgattga actgccggaa tttgatccga
     7801 gcgtttataa cgatgccatt cgtgggcaac atttgctggc aatgaaagct ctgcggcaga
     7861 aattcggcat taatgaaaac atgacgcacg tagaaaaagg tctgccagaa gaggtgattc
     7921 ctgatttggc ggagcactta caggcgggga ttgtggttct gggcacggta ggacgcaccg
     7981 gtatttcagc agcattcctc ggcaacacgg cggaacaggt gattgatcat cttcgctgcg
     8041 atctgttggt cattaaacct gaccagtatc agacacccgt tgaactggat gacgaagaag
     8101 acgattaacg actcttcaat aactgacaag gcccgagtta tcgggcctat tttttactta
     8161 ctgctcttcg ccgccaagga aataaattcc caacgggatt gccagcaaca cagtaaagac
     8221 caggctataa acaaaaatca tcgatgactg aatcacatac attgatgtcg tccactcaga
     8281 cagcggaata ttgtactgtt ctataacgcc gccgatagtc gcgcgagtca caaccgacgc
     8341 cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc ttccctttcg ctgacttcag
     8401 tttcgccata atcatgatgt aatccttgtg cagacaaatt tcttgttacc taaacgtaac
     8461 aataccatga catttcaccg ttgtctgctc tgaaaacctc acctcttagc tggatttaca
     8521 atcaacgcat tgattagtta gctaatgttg ctacttcctt ccctgactat tgatgatctg
     8581 aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc ggcatgacga caattatctg
     8641 gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa ataatgcgct taatctggtc
     8701 attattttcg gtagctgcgc agcattgatt ctgatgagct tttggtttcg ccgcggaaat
     8761 cgtaaaagaa aaggtttttt attccatgcg gtgcaatttt taatctacac cataattatc
     8821 agtgctgttg gtagcattat taattatgtt atagaaaact ataaactcaa atttatcacc
     8881 ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg ttattctgac gattaagcta
     8941 tttttgctga ttaatcagtt tgaaaaacag cagattaaaa aaggtcgcga tatcaccagt
     9001 gcgcggatta tgtcgcgcat tatcaaaatc accattattg tggtgcttgt tctgctttat
     9061 ggcgaacatt tcggcatgag cctttctggc ttgctgacct ttggtggtat tggtggtctg
     9121 gctgtcggta tggccggtaa agatattctg agtaatttct tttccgggat catgctctat
     9181 ttcgaccgtc ctttcagtat tggcgactgg atccgttcac cggacagaaa tatcgaaggt
     9241 acagtagcgg aaattggctg gcgaattacc aaaattacga cctttgataa tcgtccattg
     9301 tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa acccaggacg aatgaccaac
     9361 cgccgcatta ccacgaccat tggtttacgt tatgaggatg cggcaaaagt gggcgttatt
     9421 gtcgaagccg tacgtgagat gctgaaaaat cacccggcca tcgaccagcg acaaacctta
     9481 ctggtttatt tcaaccagtt tgccgactcg tcattgaata ttatggttta ttgctttacc
     9541 aaaaccacgg tatgggctga atggcttgct gcacagcaag acgtttattt gaagattatc
     9601 gatattgtgc agtcacacgg cgcggacttt gccttcccca gccagacgct gtatatggat
     9661 aacattacac cgccggaaca ggggcgctaa atcagacatc actggcgtta tccacaaggg
     9721 taacgccagt tcccatcaca tcaatgcttc acaatataca tagtccggct gtacgcaaca
     9781 tcttccgggt tattaatggg ataacctttc agccacggct tgattaatcg tccattggta
     9841 tatggataaa ttggtgcaat cggtgcttgc tccatgagga ttttttctgc cgcgttgtaa
     9901 tcggcattac gcgctttaac ggtattttcc gtcgatgcct gggccagaac tttgtcgtat
     9961 gccggattgt taaagcgtga aatatttcct gaatgcgttg acgttaataa tgtcaggaaa
    10021 gtggagggtt cattataatc ccccacccat gaggcgcgga taacatcaaa attgccggtg
    10081 ttacggctat cgatataggt tttccattcc tgattttgca atttaacatc tacgccaagg
    10141 ttctttttcc acatcgatgc tacagcaatc gcaatttttt gatggttttc tgaagtgtta
    10201 tacaaaagcg tcagcttcag cggtttttgc ggaccataac cagctgcgct caacaaagtt
    10261 tttgcctgcg cattcagttc ttcctgactc atttgttcaa acggcgaagg ttccggcgta
    10321 aatccggcgg taacatctgg tgtaaaatgc cacgctggct tttcgcccgt tcccaacact
    10381 ttttcggtca tcaggcggcg atctatcgtc atacttaatg ccaggcgaac gcgctgatct
    10441 gccgtcggcc ctttttgcgt gttaaacgca taataatagg tcccgagctg tggcggcgta
    10501 taaacctgcc ccggaatatc cttcaacagc ttctgataca tatttttcgg gaaggattcg
    10561 gtgatatcaa tatcccccgc gaggtaacgc ttagtggctg cggattcctg attaattggc
    10621 aggaaggtca ctttttgcag taccgttttg gcgttatccc aataatgggt attcggtacc
    10681 acgaccagtt tttcattgac tacgcgatct ttaagaacat aagcgccatt gccgatcaga
    10741 tttccgggtt tcgtccactc tttaccgctt tctacgttgg ctttttgcac cgggaagaag
    10801 gcaaagttag cggttaaatt cacaaaccac ggcaacggtt tatcaagctg aattttcaaa
    10861 gtatgggcat caactgcggt gacgccaagc tggtctggcg tagctttacc atcaataatc
    10921 gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg caaatggcga tagcgttttc
    10981 gggtccacca gacgttgcca gctgtagaca aaatcttgcg ccgttaccgg tgtgccatcc
    11041 gcccattttg cgttatcgcg cagggtaaaa gtccagatac ggttgtcatt acttttccac
    11101 tgagtcgcaa cgccggggac aatctcccct ttctcgttct gattcaccag tccttcaaac
    11161 agatcgcgaa tgacctgaat ctctggcagg cccacggctt tagcgggatc cagcgacgca
    11221 ggctcatctt taatgtggcg caccagctcc tgcttctctg ccagtactgt gccgctcgga
    11281 acttctgcag catacgaaag agaaatgctg ctgaccaaca gcgcacaaca cgtgactgaa
    11341 acagagtgct tcataagttt ccctctaacc gacattaaaa ttgatgcgta attatttgtt
    11401 tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg acacaggaag cataaaatgt
    11461 tttatgtatc aatatattaa atcagattta gtgtaaataa tcgacgccaa aatgcggtaa
    11521 agcacataga acaccgaaca ctacgttcag gcttcgcacg ttcaaaccag ttttcggtat
    11581 cataatcagc atctgtcgcc attgtacgaa gaataaaaaa taaccaaaac gttgaaagga
    11641 actgagaata cgttaagaga gaaattaaat taatgtatca gcaagtagtg ctaaacagca
    11701 cctttatact tcagcctatc tataaataat gagaatgccg agccagcatg tcgacggtgt
    11761 ggatagtaca ggtgataacc gggtaaatct ggtgtgaatt tatccaaaac acgtatcagt
    11821 tttttttctt taatagcccg ctcaacctga tcataaggta gatacgccaa tccatgccca
    11881 tcaattgcag catcaatgat caggtctatc gtattcagta aaagctgacc ttccatgcga
    11941 acacgaactt cacgtccacc gcgtattaat ctccagcgat ttgctgtacc cgatgtggga
    12001 agatacaaat taattgcctg atgatctatt aattgtgaca ctgacgttgg aacacttcgg
    12061 cgagaaaaat aatccggtga gccaacaata gccattggta tatctggccc gattcgaatg
    12121 gcgatcatat ctttatccat ctccccaccc agacggacgc ctgcatcaaa acgttcagaa
    12181 acgacatcgg tcaaaccata atcaatggtg agctgaatat caatttcagg atgcgatttc
    12241 aggaatgtgc gcattgctgg taacaatatc gtttttgctg catgttctac agtagtaata
    12301 cgtattgtcc cggatgggcg gttctgcagc tcgctcaggg atgccagggc tgaatctatg
    12361 tcatgcaaca tagggccaag aacagacaaa agatgctcgc ccgcttcagt tggaacaacg
    12421 ctgcgcgtgg ttcgcgtcag aagccgcaat cccaatcgtt cttctatacg acgcactatc
    12481 tggcttaaag ctgactgcgc catgctcagg cgggctgctg cacgagtgaa gctacgctcc
    12541 tctgcaacga cgacaaacgc catcagatca gcgatttctt cacgcttcat tcagtatctc
    12601 catgcaatct attaatcatt taaatgatag gtctaatcat gattttgggt ctaatcaact
    12661 taatgatcaa tatctatagt gatatcacca accgttaaaa cgaaccgaca actccatgag
    12721 gaacggaatg atgaataata aagtcagctt cactaacagc aataatccaa ccatctcttt
    12781 gtctgcagtg atctatttcc ctccaaaatt tgatgaaacc cgtcagtatc aggcaattgt
    12841 ggtatctcac ccaggtgggg gcgttaaaga acaaacggcc ggaacctatg ccaaaaaact
    12901 ggcggaaaag ggatttgtca ctattgctta tgacgcatct tatcaaggtg aaagtggcgg
    12961 cgagccgcgt cagttagaaa acccatacat tcgtactgaa gacatcagtg ccgtgattga
    13021 ttacctgaca acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg gaatttgtgc
    13081 cggtgcagga tatacagcca atgctgcaat tcaggatcgc cgcatcaagg ccatcggtac
    13141 cgttagtgcg gtcaatattg gttcgatatt ccgtaatggt tgggaaaaca atgtgaaatc
    13201 tattgatgcc ctgccatacg ttgaagcggg ttcaaacgcc agaaccagcg atattagcag
    13261 cggagaatac gccattatgc cactggcacc aatgaaggag tccgatgcgc cgaacgaaga
    13321 actgcgtcag gcctgggagt attaccacac ccctcgcgcg cagtatccaa cagcaccggg
    13381 ttacgctact ttgcgcagcc ttaaccagat tattacctat gatgcttacc atatggcgga
    13441 agtgtacctg actcagccga cgcagattgt ggcgggcagc caggcaggga gcaaatggat
    13501 gagtgacgac ctgtacgatc gagcatcaag ccaggataaa cgctatcaca ttgttgaagg
    13561 cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa tttcagtatt
    13621 agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa tagtgatatg
    13681 gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc aaaacagtat
    13741 ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtc tggcaatatc
    13801 tatggcaaag cattggctga aaaaggctat ctcgttctcg cttatgacgc cagtttccag
    13861 ggagaatctg gtggggagcc tcgctggata gaagatccga cacagcgcgt agaggatatt
    13921 agccgtgtta tcgattatgc agtaactctg ccatatgtta atgctgaacg tattggcgtg
    13981 ctgggagtgt gggggtggtg gctatgccat taatgccgcg ctgacagaaa aacgaattaa
    14041 agcagtagtg agtattacgg gtgttaatat cgggcgtctt tttcgggaag gtttcagcaa
    14101 ttacgatccg attggcgcat tgaatgcaat ggcgtcgcaa cgaaccaaag aggctcgtgg
    14161 tggagaatta cagatcaacg aattacttcc cgccagcctg gatgcagcaa aagcccatgg
    14221 actgaccgaa cgcgatgttt atgaagcaac tgactactat aaaacaccac gcgggcaaca
    14281 accgggaggt gcgacaaaaa tgttgttctc acatgcacag aaaacgctgg cctgggatgc
    14341 cttcgcattt actgaagtgt tactgactca gccggtgatg gtagtagttg gtgaaaaagt
    14401 cggtgccttc ggtgcttatc gtgatggact ggaagtctat ggccgggcga tggtttcgca
    14461 ggatcgtcag ttagtttcat tacccgattt ctcacattat gaactgtatg acaaaccgga
    14521 agcagttcag aaggcgctgg aaaaagttat tccttttttc aacacccatc tgggataagt
    14581 ttcatgagaa atattatgaa aaatgtactg attcttggtg ctggcggtca gatagcccgc
    14641 catgtgatta atcaactggc agataaacaa accattaaac aaactttatt tgcccgtcag
    14701 ccagcaaaaa tccacaaacc ctatccaact aatagcaaga ttattatggg agatgtactg
    14761 aatcatgcag cactgaagca ggccatgcag ggacaggacg ttgtgtatgc caaccttacg
    14821 ggggaagatt tagatattca ggcaaattca gtgatcgctg cgatgaaagc ctgtgatgtt
    14881 aaacgtctga tctttgttct ttccctcggt atttatgatg aagttccagg aaaatttggc
    14941 gaatggaata atgcagttat tggtgagccc ctaaaaccat tccgcagggc tgcggatgca
    15001 attgaggctt ctggactgga atatacaatt cttcgccctg cctggctgac tgatgaagat
    15061 attattgatt atgagcttac ctcacgcaat gaaccattca aaggtacgat cgtatcacgt
    15121 aagagtgtcg cagcattaat cacagatatc atcgataaac ctgagaaaca cattggtgag
    15181 aatataggta tcaatcaacc cgtgactgat ggtgacaagc cattattcat gtaaatcaat
    15241 tacatagtta ccatcgtaat gtactatgtg cagtacgatt tattaaactt attaaggtga
    15301 actgatcaat ccgatgcgat taacatgtgt agaaccagta agaataaaat acgccatgta
    15361 tataacatta cgacttacat ctaagtcagc ataattctaa tatctgcaca tcaataaaaa
    15421 tgcaaacaac ttccggcaaa aaagtgatac aagatcgatg acatagcgtt gaagaaaagg
    15481 atttgataat ccgcaaaatt tgcacaacac tgcctgtaat acttcctgaa ccagagacga
    15541 tcatgaccgt aaaccgccca cgcgccgaac gcggcgcatt tccgcccgga acagaacatt
    15601 acggacgttc attattgggc gcgccgttaa tctggtttcc ggccactgca gccagtcgcg
    15661 aaagtggttt gattctggct ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct
    15721 cctgtgctct gcggactttg acaccttctt tgcgccgcca tcatgtagtg ctgtgtgtga
    15781 atcctgacgg ctgccagctg gggttacggg ccaatgctaa tggtgtggat ttaaaccgaa
    15841 actttccggc ggcgaactgg aaggaaggtg aaacagttta tcgctggaac agcgccgctg
    15901 aagaacgtga tgtcgtattg ctgacaggtg acaaacctgg ctctgaacct gaaacccagg
    15961 cgctgtgcca gcttatacac cgcattcaac ccgcctgggt ggtctcattc cacgatccgc
    16021 tggcctgtat tgaagatccc agacacagcg aattaggcga atggctggcc caggcgtttg
    16081 aattgcctct ggtaaccagc gtcggttatg aaacacctgg ctctttcggt agttggtgtg
    16141 cggatctcaa cctgcattgc atcaccgcag agtttccgcc catctcctcc gacgaagcca
    16201 gcgaaaaata tctctttgcg atggcaaact tgctgcgctg gcatcctaaa gatgcaattc
    16261 gcccgtcgtg aactgaagcg ccggttccac atctaccgcc agccaggtcg gtccgtcaag
    16321 atcgacgaaa ctgacctgcg gcaccagcgg taaagcggcg ctaatggccc gagaggtaca
    16381 caacatgcag cccagcatca gacgaaaacc ttgtgcacgc gcttcagtcg ccagcgccag
    16441 cgcttccgtc agaccaccgg ttttatcaag cttaatgtta accatctcat agcgcccttt
    16501 cagcgccttc agattgctac gtgtatgaca actttcatct gcacaaatcg gtaacggatg
    16561 aataaaattc tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac
    16621 gcctaaatcc gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt
    16681 tgcgtcaacg atcagcgtcg catcgggcac agctgtgcga attgccacta tccgctcact
    16741 gataagatgg ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga
    16801 tgcactattg gccatctgat caggcgtacc gatgactacc gtctgtgcag tggtgactgt
    16861 cccgggaagc gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgcggccag
    16921 atcccacaat gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa
    16981 ctcctcccgt gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac
    17041 cgaggcatca ctttccccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc
    17101 ctcttcttcc agttcaacca ccaccacgcg cgcttcactg cgacttcccc gggcaatcac
    17161 aaacggggta tgtaagggcc aggcttcctc aaatacctta acggttctca tctttactcc
    17221 ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt
    17281 agcataaact aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac
    17341 cgttcatttc cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa
    17401 agcgcagact tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc
    17461 gggtaaacgc aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc
    17521 agtacgtaag tttaaccaac tggcaactga gatcgacaac accgttgtgc tgtgtatctc
    17581 tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat
    17641 caccctctcc actttccgta acgctgaatt tctgcaagcc ttcggtgtgg caattgctga
    17701 tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt
    17761 gattttcagc cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc
    17821 tgtactgaaa gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct
    17881 tactcttcgt tcttcttctg actcagacca tattcccgca acttattggc aatcgcggta
    17941 tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg
    18001 gttaataccg agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct
    18061 tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca aaatatcctg tggacgcagc
    18121 tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt tctttaactg ccgcacattt
    18181 cccggccacg cataacgcgt aagtacagta ttcaggtcag cggccagttt cggacgcggc
    18241 acgccctgct cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat gatgtcctgc
    18301 ggacagtcac gtagcggcgg cagattgagc gtcaacacgt tcagacgata atagagatct
    18361 tcacggaaca tgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc
    18421 cgcacatcga catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaagg
    18481 aaacgcagta atttcgcctg catccgtggt gacatttccc ctatttcatc caacagcacc
    18541 gaaccaccgt tcgcctgctc aaagaatcct ttcttccctt ccggagcatg accaaacagt
    18601 tcactctcga ccgcatcttc cggtatagac gcacagttca gcgccaggta aggtttgccc
    18661 gctctggggc ttgcctgatg gcaggcgtag gcaaagagat ctttacctgt acctgtgtca
    18721 cccgtaatca gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacatgc
    18781 ttcattttcg ggctgacggc gacaatttga ctgaaggcgc tgacgtcctg ggcggcgaca
    18841 ttttgcaact ggcggcccat acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg
    18901 acgtgttgat cattttcatc ctgaagataa acaggcgtaa tctccatcag gaaattctgc
    18961 ccattaataa cgacatgctc gttatgggaa tcttgcggtt cgctttccag ccaacgtaaa
    19021 aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc
    19081 gcaaaaagct gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag
    19141 agcacaggtt cgggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa
    19201 ggcatccacg ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc
    19261 agactgctga aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga
    19321 tcaatctcaa taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg
    19381 gtcagaccga gtcggtcttc acaaaagact tccagacgca tgggaacctt cacctgaaaa
    19441 aagaacaatt aatatgatga tatgacacta tttggatagc aggaaggttt ctgtcaacaa
    19501 tcattgacac aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg
    19561 atattgcatt atttttcgct gggcgttttg cctttttgca gcgtttcttt cacctgaccg
    19621 ataagctgac gacggaaatc cccgaggcga ggtttgtcat cgtcaatcca cggcagcggg
    19681 cggcaaagct ccatagcttt aatcccgagt cgtgccgtca gaagtccagc accaattccc
    19741 tgagctgcgc gggtagacaa acgagcagcg agatcttgcg acatccagtc catccccact
    19801 tcgcgcacca gttcgctggc tccggcaaaa gcgatattca gcaataccag cttaaacaga
    19861 cgcaaacggc tgtaataccc cagttcaatg ccatacagcg tggcgatgcg attaattaaa
    19921 cgcagattgc gccaggcgat aaacgccata tcgaccaacg ccagcgggct gaccgcaatc
    19981 atcaacgttg attccgccgc cgaacggctg atttcgcgcc gcgcctgggc atctaaaact
    20041 ggctggacca aatgcgcata caaactgacc acttcacggt cgttttgcgt ttcatggatt
    20101 gaggcatacc agcgttgcag cgctggatga gactgatcaa tacccgcctg ctgcgccagt
    20161 ttttcgcaaa atgcgcggcc tttgcccgtg ccgtggctgt gcaacaaatc gcgcgcttcg
    20221 tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa
    20281 cctacgccag cgccgataat caatgcccca gcggcacatc cacccagcgc cacccagtcc
    20341 tgagtttgcc aggcattcat tgtccactgt acaccctgcc cgacaacgct tgcgccaaac
    20401 agagccagcc cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgca
    20461 tccattaccg cttcaacttg cccctcttct tcaggcgctt catcgagcgt ggccggggca
    20521 aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgatcg
    20581 acctccagcg gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat
    20641 ctcctattaa aaattcaagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat
    20701 ccatcacctg cgggcgaaac gcttcaaact ggaacccttg cttgtcccag aacgcctgac
    20761 cgggcaaacg cgcaggaact tcaccgggat aaacggtgag tggcgcacca tcgctgagcc
    20821 ggttaccgcg caatgccgga attttctcgc cgttgacgtc aataatgccg ctggtggtcg
    20881 cctgcaccga ggccagcccc aggcaatcca tactaatccc ttcaaacgcc gcattttgcc
    20941 aggcatcctg aatcagttgt tgcagtaatg aaaccatatt ggcgtgctga tcgatggtca
    21001 catggtccgc tttagtggca gcaaacaata gcttatcgat aaccggcgaa aacaaacgcc
    21061 ggaacaaggt acgctgcccg tagtggaaac tttgcatcag ctgcgtcagt gccagacgca
    21121 tatcattaaa tgcctgtggc ccactgttga gaggttgcag gcaatcaacc agcacaatct
    21181 ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt caccaccttc tcgcagtaat
    21241 aattaaaccg ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg
    21301 actcgcccca ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca
    21361 tatctcccgg caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt
    21421 ggtggagata atcggtccac gcggcggcaa tgtctgccag ccggttttcg tcggcaggcg
    21481 ctagcgggtc cagcccttcg cacatcattc gccatttgac cgaccattcg ccgcgctgac
    21541 cattgagtaa gcccgtcatc tggcgtgacc agcttaaata gtcctgcgcc agcatcggca
    21601 ggtcgagcaa ccattcgccg ggataatcca caatctccag atacagcgtg gaggtgtcct
    21661 tgaagtggcg tagcagcgaa tcgttcgatt taaaacgtag cgccaggcgg atttcactga
    21721 cgccgcgcgt tggcgtcggc caggcgggag gatcgccata taactgcgcc agtccttcat
    21781 catatgtgaa gcgcggaatg ccaaagtcac gctgaggaat gcgttttacg cccagcaggc
    21841 gctcttcacg caccgcgctt aacagcggca agcgtgctcc ggcgtgaata ttgagcaact
    21901 gattgaccat cgcagtgata aacgctgttt tgccgctgcg gcttagtccg gttacggcga
    21961 ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt aagttcattt ttaagtcgct
    22021 tcattgctgt cctgtgctgc tctgaagacc agaaaaaaat cattcgtaag gatatgaatg
    22081 tgggggccaa gccattgatt atcaactgcc cgtgttgtca tcgacgggcg aactccataa
    22141 gatgtgcagc attcacgttc ggacggagtt cctgacctat gtcccctact atttatgata
    22201 ttgccagggt tgcaggcgta tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta
    22261 atatctcccc ggaagcgcgc gaaaaagtgt tacgggccat tgaagaatta cagtatcagc
    22321 caaacaagct ggcccgcgcg ctgacctctt cgggttttga tgccattatg gtgatttcta
    22381 cccgttcgac caaaactacg gcgggtaatc cgtttttctc tgaagttttg catgccatca
    22441 ccgccaaagc tgaagaggaa ggtttcgacg tgatattgca gacgtcgcac aatccggcag
    22501 aagacttaca aaaatgtgaa agcaaaatta agcagaaaat gattaaaggc attgttatgc
    22561 taagttcgcc agcggatgag tcattttttg cccaacttga taaatacgat attcctgtcg
    22621 tggtgattgg caaagttgaa ggtcaatatg cccatgttta ttctgtcgat accgataatt
    22681 ttggcgacag cattgcgttg accgatgcat taattgaaag tgggcatcaa aatattgcct
    22741 gcctgcatgc tccgcttgat gtccatgttt cagtggatcg ggtaaatggt tataagcaga
    22801 gcctggctgc gcataatatt gcagtgcgtg atgaatggat tgttgacggc ggttataccc
    22861 atgaaacagc actgcaagcc gcgcgggaat tattaagcca gtcaccgttg cctgaggcag
    22921 tgtttgctac tgacagcctg aaattaatga gtatttatcg tgcggcagca gagaaaaata
    22981 ttgctattcc gcagcagtta gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt
    23041 taacgcctgc gccaggcggt attgatatcc caacacagga gttaggtcaa caaagctgtg
    23101 agttattatt ccgcttaatt tctggaaaac cgtcaccaca gaatattacc gttgccacgc
    23161 atatgacgct gaaataaata ccagctacct tgcctgaatg ccggatgcga tgctagcgca
    23221 tcttatccgg cctacattta tagttcggat aaagtactcg tgccgcatcc ggcaatttag
    23281 cccattatca gaacgagtaa tttacgccga cacctgcata atggaattta tcgctgtcgc
    23341 cttcgtcgtg atcctgccac tcaaacgcgt attccagcga aacggaaagc ccgttctgga
    23401 aatcataacc gtaaaataaa cctacacggt taaaatcatg gccttcacgt tcaatatcat
    23461 cctgccagtc ccagttactc cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt
    23521 ggttgccgag cgtcagcggc aaataggcgc gaatttcttg cgtagaaaac tcaccgttat
    23581 tgcggctgtc gtccatatta aagccgcgct cgagataata gttcactcgc aaggcaaccg
    23641 tttcgttgaa ggtatattgc aggcctgttt ccgtttcgac tcgggtatca gcgtaaccgg
    23701 tcgtgttcag atcgttggca aatttataca tcgacaacca accgttgaaa cgtaaatcgt
    23761 cagtcagttt cacatcccag tctggcgcga ttttccagcg ctgcatattc gccgtgtctt
    23821 tacccggttc atcaacgtag tgataaccat aattacggaa accgccggtc aggccgaaac
    23881 tgaaatcatc gttttcgagg aactgataat gcacctccag ctccgggcga tcaaaccacg
    23941 ttccacgttt acccgcgcta taatctaccg gcccttcctg ataataggcc agagcaattc
    24001 tccacggccc gttggcggca ttaaaataga ctgaaggctc cgccagccca tccatatctt
    24061 cgccataacc ctcgacgttt tctatttcgt acatcgcgcc gatattaaag tgccagtcgt
    24121 tcctttcctc ggcctgtgcg caggccattc ccgcacacat caccagtgcg gtacagggta
    24181 ataacttttt cattattgtt ccttgtatta ttctggggga atgccttccc cctgtattta
    24241 gcgaattgct atttccgttt ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat
    24301 agtgatattt tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg
    24361 ccccccaacc gtggtgtaga gcataaattc cgcaccggtt aattctgcca cgctaatttt
    24421 ggcggaaatg ttattttcct cttgcgcgtc cggatgaata tcttccggac gtattcccat
    24481 cacgatgggc ttatgcaaac ttccctgtgt tttcagaacc gctaatttct cttcgggaat
    24541 ggttaattta agcgtttccg taacgaattt atcgccatcg atcgtgccac gaataaaatt
    24601 catcgccggt gatccaataa atccggcaac aaacatattc gctggttggt tataaaccgt
    24661 tttcggcgca ccaacttgct gaacaatccc atccttcata atcacaatcc gcgtcgccat
    24721 ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg gtggtattca gcttctggtg
    24781 tagcttgctg atctctgcgc gcatttgcac gcgcagcttg gcatcaaggt tagagagcgg
    24841 ttcatccatt aaaaacacgc ccgcttcgcg cacaatcgcc cgcccgagcg ccacacgctg
    24901 gcgttgcccg ccggaaagcg cgcccggctt gcgtttcagg tactcacgca ggccgagaat
    24961 ttgcgccgcc cagttcaccc gctcatcaat cacctctttg gcgatttttt gcatcttcag
    25021 gccaaacgcc atgttgtcgt aaaccgtcat atgcggatac aacgcgtagt tctggaacac
    25081 catcgctata ttgcgtgctt tggctggaac gtcattcatt cgtttgccgt cgatcaacag
    25141 atcgccgccg ctgatctcct caagcccggc aatcatgcgc agggtggtcg acttaccgca
    25201 gcccgacgtg ccgacaaaca cgatgaactc tttatcggca atttccaggt tgaagtcctt
    25261 caccacatgc acctggttat cgtagatttt ttgaatatgt tgtaacgaaa gctgagccat
    25321 gttgattcct ttgctatacg ttttgccaga aggccgataa ccgcgaccag gtcagagagt
    25381 ccgttgaagg caacagtaat tgcgccccgg ttaagcccgc gccgatcccc accgagcgca
    25441 taccgctggc gttaatggcg tcaatgcccg cctgcgcatc ttcaatgccg atacatgcct
    25501 gcggcggcac gcccagcccg gcacaggcgg cgagaaagat ttccgggtcc ggtttcgagt
    25561 ttttaagttg ggaagcatcc gcgcagaagg tgaaaaactc gcgcagctcc agcgccgcta
    25621 aaatcgtcgg cgcattcagg gagacagaag ccagcccaac cgggatctgc tgtgcacgga
    25681 gatctgccag caaagagcga atgccgggta gaacagcgct gaccgtcaac tcgcgcagtg
    25741 agtggacata gagcagattt ttgcgatacg ccagttgcgc cctctcctgc gagttaaagt
    25801 cgccctcttt gcccccgtgt tgcagaatgc gccgcagaga ctcatcgcgg ctgatccctt
    25861 ttagggattc gttaaactgc gcatcaatgc tgatgccaat ttcagcggca atctgctgcc
    25921 acgcctggaa atgcagatgc gcggtatcgg tgattacacc atccagatcg aaaattaccc
    25981 cttgcagttt cattcatcct cctgatgtgt ggtagcggtc ccattgaagg gcaaaataaa
    26041 atcacccaaa cagaaaacag attcttcagc cacggatata agctgaccgt tcaaacgcag
    26101 tgaaacgggc gcagaagttc gaatcgcaat acgctgcgcg tcaagagtga cctgtaattc
    26161 gcagccctgc cagaacagag ggaaagacaa ctgttgccac tgctcaggta acgccggatt
    26221 gagatgcaat tcaccgtcac gcacgctcac cccggcaaaa ccctgaatcg cccccagcca
    26281 gatagcgcca gttgcggcag catggatacc atcatcacaa ctatgcggat cagcaccaag
    26341 atcgatttca gtcccctcgc gccagaactg ataactttgg gtcagcaggc cacagcgtgc
    26401 ggcaacaatg ccgtggattg ctttacttaa tgacgagtcg tgaatagtgc gcggttcata
    26461 aaattgcaga ttggcaagac acgatgccgc tgagaactgc tccggcagca tgtaattgag
    26521 catcaccaca tcagcttgtt tgaggatttg catctcgttc acttctgcgc gtgaataatc
    26581 cagcagaatg gtttgcttcc ccgccgccgc tttgtatttc gccagattaa tcgccggctt
    26641 agccataaac gaatcatcct gcggcaaaac gccgtcgggc tgaatttctg gcagccgcag
    26701 ttctttaagg aacatttcgg cgcgatggat aaacgcatcg tcgctacagc caaactggcg
    26761 ggcaatactc agcgcctgct ggacgttgta atacgccata tagctggtga aggcgttatt
    26821 attgacatgt tcggtatatt cgtcaggccc aataacatca tgaatttcca gacggtcgtt
    26881 aaccctcacc gcgcggctaa tccagaactt tgcagtttcc agaagtagcg ccatgccttc
    26941 atgcgcaatg aaactttcat cccccgtggt ctgccagtat tgaataaccg cccaggcgat
    27001 atcggccacc agatgatgtt ccgcctgcgc cgaggccact ttttgccgca gcccggtgcg
    27061 aatgttaatg gcggcaaatt ccggcgtctc ttcttcgccg ctgcgcgcgc tttcccacgg
    27121 aaatagcgcg ccctgccagc cgttgcgtcg cgctttctcc tgcgcgcctg gcaagttgtg
    27181 ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg ctaaacagat gaaacggtaa
    27241 caaaaatact tctgtatccc agaaaacgtg gcctttgtag ccttcgccgg ttaagccttt
    27301 tgccgcaatg ctgctgcgct cgtcgtgggc aggcgtcatg atgcgcagat gataaagcgc
    27361 ataatctaac gcttgctgat cgtgcgcttc gccgccattt accgtgatac gacgtttctg
    27421 ccaccattga cgccagtttt ctgttgatac tgcaagaagt tggtcgtaac tctgctacgc
    27481 gcacatttca agctggcgaa gcgacgcgct gccccactcg tctaaaacgg cttgcctgtc
    27541 atcccgccag tcgatccaca ccagtttttg caacgtcact gtctcgcctg catgaagctg
    27601 cgcactggta tgttgctgca aacggcgctc tttggcggta tagcattgct gcacatcacc
    27661 gctcaccata cagcaacagc tgatggccac atcactgcgt ccatcctggg tggtgtagat
    27721 cccctgcatc agatgctgac caaacacccg cacctgggtt tcgtcgagat gttggcgacc
    27781 gtggttggtt tgcgtggcgt cgatacctgt tgaaatcagc actgacgcgt cggcgtccag
    27841 tggcgtaata gtgatttcca gcacaatgag cggcagttgg tctgccgaaa caaagcgacg
    27901 gctggcgatg gtgtaacctg tgccgttgct ggtacgccag acaacgctac ggcgtaattc
    27961 tccactggca aagtcaagtt cacgctgcca ggcttcgcgg gataacgaga aaacctcacc
    28021 atttatggca atctccatcc ccaagatatc aggcaggttc accagttcgt tgatttcacc
    28081 ttttcccgcc cgatgataca gccccgccag atacatccct cgcgtctggc gcgtgtaatc
    28141 ttcttcatgg ctggcgcgaa gcccaagata gccgttcccc tgcgccatca gcgatgcata
    28201 cttgttcagg gtatgctggc tgaaatgggg ttctgataac gttactggcc tggtcataat
    28261 tccacacgcg tccctgtttc tgcggattga tacaacgccg ccaccagttg ctggatgatg
    28321 tacccctgct cggcgtcggc tatcatcacg ggcttgccct gtacgtgatt gataaaggct
    28381 tccatgctgc gcaaatggcg gttgtcgtct gccagttccc gttgcatcag cgtcattaat
    28441 tcaccgttgt tatcggtgta gatatgtgct ggaaacagcg tcgcaccggc tttatcacca
    28501 caaaagctga cgttcataat cgactgttcg cggatgttga gtgcaaatga cgtttccagc
    28561 cacagaatgc cgccgttatg aaattcaatg gtgccaaata acgaatcttc gacgctgtaa
    28621 gttgccggat cccactcgcc aaattgaccg cagctctttt gcgtgccgat cttttgaaag
    28681 ctatgcgcat tcacgctttt caccgccgga aagcccagca catacatcgc cgcatccagc
    28741 atatgaatgc cgatgtcgat aagcgggcca ccaccctgca gttctttatt ggtaaagaca
    28801 ccccagccgg gaacgccgca gcgacgcagg gcgcgggcgg tggtaacgta aatctctccc
    28861 aaaacgccgt tggtcacctg ttcacgcagc tgttgcgtat cgagcgcaaa acggtggtga
    28921 aagtcgtagg ccagcacctt gcccagtttg cgcgcggtat cgcacatttc ccgcgcctgt
    28981 tctggcgtca tggcgggcgg tttttcgcac atcacatggc agcccgcttc cagtgccatc
    29041 agggtatgtt cgtaatgaaa acggttaggt gagcagacgc taaccacatc aggtttcacc
    29101 gccagcagca tggcctgcgg atcgtcccac acggatgcat taccgtattt atctgccagc
    29161 gcctgcgcct gggaaaggcg gctgtcacag acagccacca gttccagatc gttgcgggtg
    29221 cagtagtacg aagcatgaac cttatccgcc acctggcctg cgcctattat cgcgacccgc
    29281 agcggagagc ttgtcattgc acttttcaca aagacctctt agcaggtacg caaccaggcc
    29341 aacgaatcac ggtacgcctg ggcaggatct tccgcccgga tacgcccttc gtacactaca
    29401 tagccctgat agttatccgc gcgcagctgt tcaaacagcg cgtggaaatc cagggtgccg
    29461 ctgcccggct ggtagcgatg gttatccgca atatgcacat gaccgagcag gtcgcggttg
    29521 tcatgcagcg cctgcgccag gttatcttct tcgatattca tgtgatagaa atcgccgata
    29581 atctgtacat gtttaagatc gttttcgacg atgtaacggc gggcatcggc gagggtgttg
    29641 atcatatgat cctgatagcg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg
    29701 gcgacctgtt ccagtacgtg cagggaatca ctcaccattt tgcggtcgcc gtccaggcta
    29761 cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc cccacgctgc cggaacgacg
    29821 atacctttac cccccacttc tgccagcgct tcgagaatgc gttcgatctg ctttaagcca
    29881 ttaagacgac gctcttcgat aaagtcgcca atccaaccgt catagccacc gcaggcggtg
    29941 gtcaccggta aaccggtttc tttgattgct gctttgactt cttcgagatt gttgaccagc
    30001 agtttgccgt caatctcaaa accatcgaag cccatctctt tgatataacg aaatttctcc
    30061 agaatgtttt ccggaaagaa tgcctgattc tgtgtgccta ttttcatttt ttcattcctt
    30121 tgaattaaaa cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca
    30181 tgtagctttc cgggctcttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga
    30241 gataaccgtt catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg
    30301 gataatccgg gttcggttcg ctgcacgcac gggagaaaac aattttggcg ttattgaaat
    30361 gcgcttcgcg tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataagaga
    30421 tggtgccgcc ataagcaagg ccacgcagcg ccgattgcag cgcgtcggcg taaccgctgg
    30481 tttcgataat cacatcggca ccctgcttgc cggtcagcgt tttgatctct tttcctacgt
    30541 cagtgccaat ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat
    30601 aggcgatagg atcgacgcca atcaccaccg acgcgccagc gcgtttcgcc agttggatgg
    30661 ctatttgacc aatcgcgcca agccctacca ccaccacaaa atcacccacg cgcacgttgg
    30721 catcgcgcac gccgctcatg gcaaactgcg ccgggtcgta acaaacggcg tttttccacg
    30781 agctgccttc cggcattttg cgcagcttgt agttattcac cgcgttaatg atgaccgtct
    30841 cggagagcgg gccgtagccg catacgctgt cgcccaccgc gtagtcggta acgtcgctgc
    30901 cgcactcgat aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc
    30961 cgcgcggcgc atctgccgga cgcggagtga acatctgcca ttcgccgtta aagtcttcat
    31021 cgataaacgg gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga
    31081 aacgcacgcg gattttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg
    31141 cagcaacacg cggtgctgtg gctactaact ttttcattta aaactccttg taaagaaact
    31201 ggccgcttta gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa
    31261 gcgcatacat aatcaccacc ggcaatgcgg tcaccagtga ggccgccatc atccgtcccc
    31321 agatgtaatc tggcgtactg aacagcgcgt tcaggcccac cggtaaggtg aaattgctgg
    31381 cgctggagag gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga
    31441 cgaacacgga tatcagcccg gacatcgcca acggcacggt gatgcggaag ataatttgca
    31501 gacggttgag gccatccatc atcgccgctt cttcgatctc atcggggatg gtgtcgaagt
    31561 agcttttcag cataaacacg gcggtgggca gagtttgcgt caccatggtg atgatcagcg
    31621 ccatctcggt gtcgtaaatg cccagcgcgg tgatgatttt gaacagcggc accaccagca
    31681 aaatgccaga gaacatatac accgtgtaaa agctggcatt gatggtcatc cgacctttaa
    31741 agcgcaggcg ggaaagcgcg taagccccaa gaatgccgag gaataccgcc accacggatg
    31801 aaaccaccga caccaccaga ctgttacgga agtagtcgac aaacgggaaa atcatcgggt
    31861 taaaaatgtc gacgtaatgc tccagcgtcc actgctgcgg cagcagcgta ggatgcagtg
    31921 agatcgcctc tttcgcgccc ttgaacgagg tcatcagcat cacaaaaaat ggaaacagcg
    31981 tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat gcgactgagt gtgcgtttat
    32041 ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg
    32101 aagagcacca ccgagatcgc cgccgctttt cctaaatcat taaaggcaaa ggcggttttg
    32161 tagagatata caccgagaat atcgaccttg gtcgtcagca aataaacatc cgcgaacatg
    32221 tagaacatcc agatggtgcg cagggtcacc accgtcgcca ggaccggcat aatcgcgggc
    32281 agcgtgacga tacgaaaccg ttgccaggca ttagcgccat ccatctctgc cgcttcgtac
    32341 agcgatttgt cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag
    32401 cgccagatgg cgaacagcac caccagcaca aagctactgc ccggattatc gaaccacagc
    32461 ggtgcctgct catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg
    32521 ttgttgaaca tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg
    32581 atcaccagcg aacgcgcggt tttgcgcaga cggaactcgc ggttgaaaaa catcgccact
    32641 gccagcccga gcacggtgct gcccgccacc accagcgcgg tataccagac cgtcatccac
    32701 agcgagtgcc agaagccggg atcggagagg atacgcacat agttactcac cccaacaaag
    32761 gttgactcga tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc
    32821 attggccagg ccaccagacc acccagcagt aataagctgg gcgcgagaag cagcagcgca
    32881 aagggcatat cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt
    32941 agtgctgttc gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat
    33001 cggctttacc cacggtgacg ttatgcacca tggagctcac cacgccagac cccgtcacat
    33061 cacccatgcg ggtaaagttt ttatccccta ctgcaccaaa gacctgaata tttggcagtt
    33121 caccgattag ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg
    33181 tggcggtagt gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc
    33241 agtcggcaat gttgtctgcc tgctccataa aggtgacaaa tttttctgct gcttccgtct
    33301 cttcggtctt ttgcccggcg gtaatggtca gcgaggtcaa catgccgtag accgcagagt
    33361 ttttctcggt tggcaccacg aaaccgacgt ttttcgggtc gccttcttta atcacagccg
    33421 gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc gttcataaag gcgtctttca
    33481 cttccatgat gtcgttagaa cccggcatag tgttggcagc aaggtcgcgg taataggtca
    33541 gtgcctgcat catctctggt gtatcaaggg tgattttgcc ttcggcgtta aagacgttag
    33601 cctggttgga taacgcaaac tgggagaagg attgttccgt caacacgctt tctgctgtag
    33661 gcagcgcaat gccgtatttt ttattcgccg ggtcattcag tttctgtgca acgtccagca
    33721 gctgttgcca gtttttcggc tcctcaagcc ccgcttttgc cagcacatct ttgcgatacc
    33781 agataccgcc aatccaggcg ctgacaggaa caccggtcca tgcgctacca tcttcggtac
    33841 gcacaatacg cagtacgcca tcgtaaaacg cgccttcacc aacgttgctg atgactgtgg
    33901 caaccgcttt gcgatcgata agctgctctt tgtccatcac tttggcgtag tcatggctgg
    33961 tttctatcac ttccggcagc gaaccgctac gtgaaagagt aatgacttta gtgttatagg
    34021 catcttcttc cacgggcact tgcttaacgg tgatgccagg gttttctttt tcaaaacggg
    34081 caatcaattt actgataacg gcctggcgct cctgctccac cgaagaatgc ataaattcga
    34141 tggatacatt cgttttattt tcttctttac agcctgaaat cagggcgcag gaaaccagtg
    34201 ctgataacag cacgattttt gatttaatca tgagatgttc ctttttattt aatccacata
    34261 acctgccagg ggcgcagcgt tatttcatta cccaatatat caacttcact aatcagatcc
    34321 cgaccatgca gattagtaat atttacatgc tgaatatttt tactgacatt aaacaatcca
    34381 gtcagacaat taccatcagc gttacttctt tgaatacgca ttacggatga attaatcgta
    34441 tcgatggtaa aattattatc cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa
    34501 cgcgacaact catgatatac cgcatgcctt aatgtagctt catcgttcag ttctcgggtt
    34561 atctctttac tgtgatattt tttacggtta atcgcacggt tatatccgag tttttcgaca
    34621 cctgcgtaat cattacgcga gccaagaata ctttgaatat atatcgctgg cacaccgggg
    34681 aaacttaaca aaatcgcatg ggcgagaata aacctggcgc aacgttcttc atcgctactc
    34741 tcacggcggc ttaacgcatc catataggtc acatttattt catacggact gcgtgtaccg
    34801 tcgggattat ttttccagtt tactaatgca ccttcctgct gtaacgcctc gaccagctct
    34861 aatatttcgc tttcaggcaa caagccccgt agcgggttta gcccgatgcc atcgtgagag
    34921 gcgaggaagt taaaccaggt ggtgttgctg gaaggtagtg tcaggttttg cgcccacgca
    34981 caaagcgcct caacgttctg tttttgcacc gcatgcagca ccagtggcgg cagcgagaac
    35041 tggtacacca tatgtgcttc gtcatcgcct gcgccaaagt aagcaatgtt gtctttatgc
    35101 ggaacattgg tctcggtaat gatcactgta cctggcgcaa cgttatcaat aatcgaccgt
    35161 aacagtttga taatcagatg tgttttttcc agatggatgc agcttgttcc cggctctttc
    35221 cacataaagc caacggcatc caggcggaca tactcagcac ctttcgcaag gtaacagagt
    35281 aaaacatcca ccatcgccag caacacttca gggctacggt aattcaggtc aatttgatcg
    35341 tcactaaagg tggtccataa atggcgcgtt gaatgatcgc gcatctggaa tggcgttaat
    35401 aacggtaacg cacgcgggcg agtgacggcg ctgagatcgg tttgcgggtc aacggcaata
    35461 aaaaaatctt caaaacctgg atgctgttgt aaatagtttt taaatcattc actttttgcc
    35521 gacatatggt tgcagacaaa atcaaacatt aaatgactgc attccccgag ttgctgaata
    35581 tcctgccact cccccgcttc actggcgacc tgatgataat caattaccga aaagccatca
    35641 tcagatgacc atggataaaa cggcaacaaa tgaacatgtg aaaaaatgct ttgcagccat
    35701 tgatggtaaa actgattaaa tgtgggtaat ggttttaaat cattgctgtg aaattgatcg
    35761 gcataggtaa ttaacacgac atcactttca tcccagtgtt ttttacgtcg ctgtgtaatt
    35821 aatcgtttcg cactctcaag acgcgttaca agtttctgta aatgagttgc ggtaaatgtt
    35881 ccaccgtaga tttcgtccag gtaatccgta attttctgtt tcatgttgcc tttccgtggt
    35941 agcggtccca tgaaacgaga gactactccg cagaaaataa actgtcaatt gagcacaagg
    36001 gttttagtca cgcaggaaat cattttttac cagggaaaaa gcgtatttgt gagctgacgc
    36061 aaataatcta tggggatgta aataaggtgt gacagaaaaa aaacggcgca taagcgccgc
    36121 tcatggtgaa ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccaccggc
    36181 gttctcaacg tgggtatatc ccatctcgct aaggatctct tttgcttgcc ctgactggcg
    36241 tccggcattg caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg
    36301 ctctttcact tccttcaggg gaatattgat ggccccctga acgtgctctt gctgatactg
    36361 ctctggaaca cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag
    36421 tgccagagca agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc
    36481 atccattata cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc
    36541 tgtgccagtt tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt
    36601 ttcagcggtc ggcgagcaac tgattttatc gcccagcccg ctacacccgc cgggccatag
    36661 cgcagtgcgg taagaagtac cagcttgcct gctaatttga aaccaggctt taccttttgc
    36721 ccggcctgtt gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta
    36781 acgtgaaagt atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg
    36841 ccagttcacg gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg
    36901 ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa
    36961 acagcgccag accgaagaaa atcgacagca ccaccaggat acgcaccagt tttgccggta
    37021 catcaaaata gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac
    37081 gccataattt tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc
    37141 tgcatcaaga atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc
    37201 cagttgcgct aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct
    37261 gtaatgcagc cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc
    37321 cagaaatagc gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg
    37381 cctggatgtt attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg
    37441 ctgattgcat catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg
    37501 aagctgtggc tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa
    37561 cgagccattg cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag
    37621 tttgccgcct gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg
    37681 ttttccagct caccgatctc tttcttcatg cgtgccagcg tgtcgtccac cagcgtcact
    37741 tcatgttcca ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt
    37801 gccagatcct ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca
    37861 cgcgccgacg cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcgcgcgcc
    37921 gaagtagaac gtacttcaac cagtgtatct tccatctcct ggatcatcag acgaaccagt
    37981 ttctgtggat cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca
    38041 aagcgagaaa aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc
    38101 tgaactgtta atacaatttg cgtgccaatt ttttatcttt ttgatttata aagttctgat
    38161 tgaagaatca acagcaacat gccaggatga gttagcgaat tacactaaca agtggcgaat
    38221 ttcatcatgg cagaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg
    38281 ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc
    38341 ggcaccggta aagagctgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg
    38401 ccgtttattt cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt
    38461 ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt
    38521 gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag
    38581 aaattattgc gcgtgattga gtacggtgaa ctggagcgcg ttggcggcag ccagccattg
    38641 caggtgaatg tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa
    38701 ggcacttttc gcgctgacct gctcgaccga ctggcttttg atgttgtaca actgccacca
    38761 ctgcgcgagc gcgaaagcga cataatgttg atggcagaac actttgccat ccagatgtgt
    38821 cgggaaatta agctgcctct gttcccgggg tttacagagc gcgccagaga aacattgctg
    38881 aattatcgtt ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat
    38941 cgccacggca ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt
    39001 ccgcctgaag acgctatcgc cgtttcagaa accacctcgc ttccaacact gccgctggat
    39061 ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg
    39121 aaatataacc agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg
    39181 ttgttgaaaa agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg
    39241 aatgctttat tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca
    39301 tcagacatta ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc
    39361 gtatcgaatt gcgcatccgg tatggtcagc ggatacagga agcgaatcac gttgccgtat
    39421 gcgccacagg tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca
    39481 atcgccgctg acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc
    39541 aggccgcgta ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg
    39601 agacgctggc ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc
    39661 accgcgtgcg cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc
    39721 ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc
    39781 gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac
    39841 agtttaccgg tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc
    39901 tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgccc
    39961 tcgccctgca ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta
    40021 aacaagcgtt cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga
    40081 taaggtacgt gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact
    40141 tttccggtca gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc
    40201 acgccaggtc gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgca
    40261 ccggtagtga agaatgcggt tttagcctgc ccgctcactg gggcaagggc gttaattttc
    40321 tccgccagcg tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt
    40381 tgcaattgct gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc
    40441 gcaatgcctg cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt
    40501 tctgccgact gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga
    40561 cgacgctgat ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat
    40621 cggaaattaa acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa
    40681 tggcacgcgc agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc
    40741 gaaacgttcg gcgtcgccgc gcagcagttc ggcaatcaga cgcccggcaa gatgggtaca
    40801 ggtgacgccg tggccgctgt agccctgcat gtaatagatg ttggtatcaa ggcgaccgaa
    40861 ctgcggcatg cgcgacaagg tcagcaggaa gttgtccgtc cagcggtaat caattttcac
    40921 gcccttcagc tgcgggaagg ttttcagcag tttcggcacc acaaggcgct caacgtcatc
    40981 cgggtcgcgc gcgccgtaga ccacgccgcc gccgtacagc aggcggttgt cggcggtaag
    41041 acggtagtaa tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga
    41101 acgggctaaa tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct
    41161 gcgtttcgcc agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac
    41221 atacttcgcc gtcacctgac ctttggcagt tcgcacaacg gctggcgtgg tgtgctggat
    41281 ctgcgtcacg gcagaaagtt catacacgcg cccgccgttg aggcggatgg cgtccgcttc
    41341 gccaatggcg aggtttagcg gatgaatatg cccaccgctg tgatccagca gcgcaccggt
    41401 atagcgatcg ctggctactt cacggcgaat ggcgttggcg tccagcaatt ccagctgttt
    41461 attgccgtag cgttcccagt tctctttctg ctcttcaagt gtggcgagct gtttatcatt
    41521 catcgccaca aacaggccgc cggggcggta gtcgcagtca atctgataac gtttgatacg
    41581 ttcgcggatg atctcaccac cttcgaacat catgctgccg agcatacggg cggtgtccat
    41641 gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc
    41701 attgcgcccg cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc
    41761 cgcttccgcc agatgcagcg cggaagagag cccggtatag ccgccgccga ccacgcaaac
    41821 gtcgcaggaa atcgactcat tcagcgtgtc gaatggcgcg tatttattcg cactggcggc
    41881 gtagtaactg ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca
    41941 gttcggtgaa tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat
    42001 agccgccaaa cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccag
    42061 ccttcaggcg gcggctcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc
    42121 caaggccgta ctggctgtcg ttggcaagct gtagcgcctg gtcttctgat gtgaaacgcg
    42181 tgaccaccag caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtctacat
    42241 ccacaaagat ggtcgggccg atggcggcag ccagctcagc gttacgacca tccagcagca
    42301 gttgcccttt gctttcgcct tcccgaataa agctatggac cgagtcagcg tgagcgctgt
    42361 cgattaaggt gcccatggtg gttgcgggat caagtggatg gcccggctgc cagttttgcg
    42421 cctgctgttt taacagggct aagaattcat cggcgatgct ctcttccagt aacaaacgcg
    42481 ttccggcgat gcacacctgg ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg
    42541 ctttttgcag atccggacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca
    42601 gccagacgcg tttcatgttg ctctcgcccg catctttcag cagctgtttc ccggtacggg
    42661 tcgaaccggt aaaggcaatg gcgtcgatat cgttatgacg cgacagcgcc tgccccgctt
    42721 catgaccaaa acctgtcacc acgttcaaaa caccatccgg caagcctgct tctttcgcca
    42781 gccccgcgag acgaatcgca ctgagcggtg atttttcaga cggtttcagg acaacgctgt
    42841 tccctgctgc cagcgccggg ccaagcttcc agcaggtcag cagcagcgga aagttccacg
    42901 gtacgatggc ggcaatcaca ccgaccggtt cacgcacgat catcgccagc tcatggctac
    42961 tggtggtcgc cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatagcgc
    43021 gcgccgcgcc gggaatatca tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag
    43081 tttccagcag tgccagctct tcggcgtggg cttccattaa atcggtgagt ttattcagta
    43141 ccgctttacg tttagccgga gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg
    43201 ctgcgctcac cgcacggtcg atatcgacgc tcttgccgcg ggcaattttc gccagcggtg
    43261 cctgggtgac cggatcaacg gtttcaaagg tttcattttc cgccgcagca gtatattcac
    43321 cgttaataaa taagcgggtt tcaatggcga gacttaacgc tttatcctgc cagtaagcca
    43381 gatgatgaaa attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa
    43441 cgtcagggcg cagagatccc cgggccgtat gcccgtttat ttctggcgac attctttata
    43501 tacacttcat ccttcaagcc gcctctgcct tggctgcgtt cgctcccccg aatcacttac
    43561 ctgagtaaac tcatcgggat tcgctcactt gccgccttga tgcaacttga atgattttgt
    43621 gtatagattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
    43681 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
    43741 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
    43801 atcgtcagaa caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt
    43861 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
    43921 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg
    43981 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
    44041 agtgacagac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
    44101 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
    44161 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
    44221 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
    44281 ggtgctgaca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
    44341 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
    44401 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
    44461 ccccttgccc atgcagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
    44521 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
    44581 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
    44641 aaccaccggt tgccaccacc agttcctgta aaccccggca gatggcgaaa atggggatgc
    44701 gcctttcgag tgcggcatta attaacgcca tgctcagaag atcacgcccg ggatcggcgt
    44761 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctaccaggaa
    44821 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa tgacggttcc gccagcgcat
    44881 gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    44941 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    45001 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    45061 ggtttaaatg acctttatgt tcaatatttt ttcaatctag cagtgggttt tcatttttgc
    45121 aaactcaatt taacatttga caaacattta gtttgcatac agattcgaat ggtggtcatt
    45181 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    45241 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcgccttta
    45301 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    45361 ttactgattt gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagt
    45421 tggagaaagg gtgttatttc ccggcctcgg tgtttgcaat ggatattctg ggcaacgtag
    45481 tagaagaagc gggtctgggt caggaaatgg gcgagccgga tcgtacttgt gttcctgttc
    45541 tcggttcctt aaccccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    45601 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    45661 ggcagcagct acgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt
    45721 tactggatcg tcagcgcgac gctgaagggt atctgcaacc gccctgcgcg cccggcaccg
    45781 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgatg
    45841 tgctcaatga tattgatgaa ctggcgcagt tacagttaat tcccgccgac ggtgcggtcg
    45901 ccgaagcctc gccgggtcag tttgaaatca acctttacca taccgataac gtgctggaag
    45961 cctgcgatga tgcgctggcg ctaaaacgcc tggtgcgtct gatggcagaa aagcataaga
    46021 tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcgga atgcatatcc
    46081 atatcagtat gcaaaataac cgtggcgaga acgtgctttc tgacgcggaa ggcgaagatt
    46141 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt
    46201 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc
    46261 aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggcgaccatc
    46321 ataatcaccg cgtggaatat cgcgtggcgg gtgccgatgc caacccatat ctggtgatgg
    46381 cagcgatttt tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag
    46441 tcgaaggcaa cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc
    46501 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    46561 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    46621 aaattgagtg gatgttgaaa aacgcgtaat tccccatgcg gggttgtttc atcggcctga
    46681 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    46741 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    46801 ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac
    46861 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    46921 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    46981 tgcgcaaccc ggcaaaacca gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    47041 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    47101 tagcgacggt cacgttccag cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    47161 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    47221 gcaaaagtcg attaatccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    47281 tctctttttg ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    47341 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    47401 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    47461 catggtggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    47521 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    47581 tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    47641 gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg
    47701 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    47761 taaagacccg gacgccgcac tgcctgaaat tgcgctgttc gtcggcggca agctctttca
    47821 gtcgattttc ctctgcacca cgtttgtgaa cacattagcg tctggcctgg cttcacatgc
    47881 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    47941 tggctatgtg cacccaaaat ggcggactcc ggcactcaac gtcattatgg tcgggattgt
    48001 cgcgctgtcg gcgctgttct ttgatttagt caccgcgaca gcactgatta acttcggtgc
    48061 tctggtggcg tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg
    48121 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    48181 gacggtgggt gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg
    48241 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgct
    48301 gtacgaaggt gacagaacgc cggtgagcga aacgtaatag ccggatgcgc ggcgcattat
    48361 cctgcctgag ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    48421 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    48481 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    48541 tcgcagaggc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    48601 tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta
    48661 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    48721 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    48781 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    48841 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    48901 agatggcgac gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    48961 caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    49021 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    49081 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    49141 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    49201 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    49261 gcctgatgcc ggaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    49321 tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa
    49381 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    49441 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    49501 aaagcgtccg caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt
    49561 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    49621 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt
    49681 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    49741 ggcgcggtaa accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac
    49801 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    49861 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    49921 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac
    49981 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    50041 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    50101 aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc
    50161 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    50221 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    50281 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    50341 cgacggacag taacgacccg gacagtttct tccgtccttt actgagctgc gcggcaattc
    50401 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg
    50461 cgctctcctc gcagcagctg gcggcgcgta ttgaagccta tgacgaagcg cagagtattc
    50521 tggcgcagga attgcccatt ttgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    50581 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    50641 gcaagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    50701 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcacccc
    50761 tcatgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaacggcct
    50821 gatccactgg gattttggtg tttccagcat taacggtcaa ccgattgccg aacagttaaa
    50881 agaggtcttc ccggcgacga tggaactgtg catcctcgcc ttcggttttg cgctgattgt
    50941 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcaag ataacttgat
    51001 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    51061 gctgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    51121 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    51181 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    51241 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactatcg aagtgtatga
    51301 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    51361 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    51421 catgctgacg caggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    51481 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    51541 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    51601 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    51661 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    51721 acggctgcgc ggggctggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca
    51781 tcgaccagca atttctcggt tatcaattat tgccgccgtc atggtcgcgc tatggcgaag
    51841 tttctttctt cctggggact gacgacctcg gacgcgatgt tttaagccgt ttactgagcg
    51901 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc
    51961 tggtactggg caccttcgcc ggggcgacac acggcctccg ctcagcggtg cttaaccata
    52021 ttctggatac cttactggcg atcccttcgc tgctgttggc aattatcgtt gtagcgtttg
    52081 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg
    52141 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    52201 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg
    52261 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    52321 tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    52381 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    52441 tgattagcgt gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg
    52501 gggtggaata atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga
    52561 tgagtgggtt aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    52621 tcttgttggt gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa
    52681 taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg
    52741 tctctccgca cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga
    52801 accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    52861 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    52921 tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta
    52981 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    53041 gcgtctgctg attgctgacg aaccgaccaa ctcaatggag ccaacaaccc aggcgcaaat
    53101 ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca
    53161 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    53221 agtggaaacc gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc
    53281 gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac
    53341 gctgcccggc gctatcccac tgctggaaca gttaccgatt ggttgtcgtc tggggccacg
    53401 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    53461 tctctatgcc tgtcatttcc cgctgaacat ggagaaagag tgagatgatc gaaacgctgc
    53521 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggctggttt cgtcgtcaga
    53581 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    53641 ttggcgagaa tggttcgggt aaatccacgc tggcaaaaat gctggcggga atgatagagc
    53701 ctaccagcgg cgagttattg attgacgatc atccactgca ttttggcgat tattccttcc
    53761 gcagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    53821 gtatctcgca aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc
    53881 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    53941 actatccgca tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga
    54001 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcgatgc
    54061 gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt
    54121 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    54181 agggcgaggt tgtcgaacga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    54241 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta
    54301 aagatcgcta attttctgaa tcctccttcc agattatcca cctcattctg acaagagatt
    54361 aaatcttgtc agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc
    54421 aatctcactt tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat
    54481 ttcaaatttc gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg
    54541 gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc
    54601 aggcgcacta gctggcttga gtcgcagagc tggaccgtgc tgcgtttctg gcataacgag
    54661 attgattgta atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca
    54721 ccctcaccct gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat
    54781 ggagacagtg ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag
    54841 ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc
    54901 cctcgcccct ctggggagag ggttagggta aggggaaaag cgcccaccct gccgcagcct
    54961 gctccggtcg gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt
    55021 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
    55081 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
    55141 tgccagcaaa ctatccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
    55201 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
    55261 attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat
    55321 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
    55381 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa
    55441 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
    55501 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
    55561 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
    55621 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
    55681 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
    55741 cccgattcgc cgtaccgtta ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc
    55801 cgatctctct gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc
    55861 tgcaatgaac gaactcgaat tgaaataatc attctgttgg taaatattgg gcggcgttct
    55921 gccgcccgtt atctctgtta tacctttctg atatttgtta tcgccgatcc gtctttctcc
    55981 ccttcccgcc ttgcgtcagg ataacgattt cctttacgac caaggagcgc ccatggaaca
    56041 acgccacatc accggcaaaa gccactggta tcatgaaacg caatccagta ctgcggagta
    56101 tgacgttctg cctctggtcc cggaagccgc aaaggtcagc gatccctttc tgctcgacgt
    56161 gatccttgat gaagaaacgc tggctccctt cctttcatgg ctggtccctg cgcgcgttct
    56221 tgcagtggaa ttgttccctg accagcttac cgtgacccgt tcacagacct tcaccgctta
    56281 tgaacgcttg tctacggccc tgacggttgc tcaggtttgc ggcgtccagc ggttatgtaa
    56341 ctactattca gcgcgactta cgccgctccc cgggcctgat tcctccaggg aaagcaatca
    56401 taggttggca caaatcacgc aatatgcccg ccaactggct agctcgcctt ctattatcga
    56461 caaccgatcg cgccagcatc tgaatgacgt cggtcttact gccggggact gtgtgatcat
    56521 taaccaaatc attggtttta ttggctttca ggcgcggacc attgcgacat ttcaggctta
    56581 tctcgggcac ccggtacgct ggttacccgg tctggaaata caaaactacg ccgacgcgtc
    56641 actgtttgct gatgaatcaa tacgctggcg aagcagctat gaggtggaaa aactacctga
    56701 agagtacaca aaaagttcaa cagcagaact ttgccaactg gccgaaacac tctctctcca
    56761 ccctatttca ctttcccttc tcgaaaggtt gttaaacagt acacgggtta atacacagcc
    56821 ggataataag cttgcggcgt tgttatgcgc gcggataaat ggcagtcctg cttgttttgc
    56881 cgcctgtatg gattcatcaa atgaatataa aaaaatcagc ccccttctgc gcaagggcga
    56941 aaatgaaatt aaccaatggg ctgaccgtca ttctgttgag cacgctaccg ttcaggcgat
    57001 acaatggctg acccgagcac ccgatcgctt tagcgccgcc cagttcagcc ctttactcga
    57061 acacgaaaaa tcatcaacgc agattattaa tctgctggta tggagcgggc tgtgtggctg
    57121 gataaatcgc ttaaaaatcg cgttgggtga gacatattaa ccttgccgcg tcagacagat
    57181 tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata gacaattatg tttcaggaca
    57241 acccgctgct agcgcagctt aaacagcaac tgcattccca gacgccacgc gctgaagggg
    57301 tggtaaaagc cacagaaaaa ggctttggct tcctggaagt cgacgcgcaa aaaagttatt
    57361 tcattccgcc gccgcagatg aaaaaagtca tgcatggcga ccgaattatc gcggtgatcc
    57421 acagtgaaaa agaacgtgaa tccgcagagc cagaagaact ggttgaaccg ttcctgactc
    57481 gtttcgtggg taaagttcag ggcaaaaatg accgtctggc catcgttcct gatcatccac
    57541 tcttaaaaga cgccattcct tgccgcgcag cccgtggcct gaaccacgag tttaaagaag
    57601 gcgactgggc ggttgccgaa atgcgccgtc atccgctgaa aggcgatcgt tctttctatg
    57661 ctgaactgac acaatacatc acttttggtg acgaccactt tgtaccgtgg tgggttaccc
    57721 ttgcgcgcca taatctggaa aaagaagcac cagacggcgt cgctaccgaa atgctcgatg
    57781 aaggtctggt tcgtgaagat ctgaccgcgc tggattttgt caccatcgac agtgccagca
    57841 cagaagatat ggatgacgcc cttttcgcta aggcgttgcc ggatgacaaa cttcagctga
    57901 ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg cagcaagctg gacaaagccg
    57961 cgaaaattcg cgcattcacc aactatctgc ctggcttcaa catccctatg ctgcctcgcg
    58021 agctttctga cgatctctgc tcactgcgcg ccaatgaagt ccgcccggta ctggcatgcc
    58081 gcatgacgct ctccgctgat ggcaccattg aagataatat cgaattcttt gccgccacca
    58141 tcgaatccaa agcaaagctg gtgtatgacc aggtttctga ctggctggaa aataccggtg
    58201 actggcagcc tgaaagtgaa gcaattgccg aacaagtccg tttgctagcg caaatttgcc
    58261 aacgccgcgg cgagtggcgt cataaccacg cactggtgtt taaagatcgc ccggattacc
    58321 gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt cgccgagcct cgtcgcattg
    58381 ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat ttgtgcggcc cgcgtactgc
    58441 gcgataagct cggttttggc atctataacg tgcatatggg ctttgatccg gcgaatgccg
    58501 acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt cgatgccgaa gaagtgctca
    58561 cgctggacgg tttctgcaaa ctgcgtcgtg aactggacgc gcaaccaact ggtttcctcg
    58621 acagccgcat tcgtcgcttc cagtcatttg ctgaaattag cactgaaccc ggtcctcact
    58681 ttggcctcgg tctggaagca tacgccacct ggacttcgcc gatccgtaaa tatggcgaca
    58741 tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga aactgcgacg cgcccacagg
    58801 atgaaatcac tgtccaaatg gccgagcgtc gccgtctcaa ccggatggca gaacgtgatg
    58861 ttggtgactg gttatacgca cgcttcctga aagacaaagc cgggaccgac acccgtttcg
    58921 cagcggaaat tgtcgatatc agccgtggcg gcatgcgtgt tcgtttggtt gataacggcg
    58981 ctatcgcctt tattccggca cctttcttac acgctatgcg cgatgaactg gtttgcagcc
    59041 aggaaaacgg cactgtacaa attaaaggtg aaacggttta caaagtgact gacgttattg
    59101 acgtcactat tgccgaagtc cgcatggaaa cccgcagcat tattgcgcgc ccggtcgcgt
    59161 aatctccttt cacggcccat tcctcatgga tgggccgttt atttccccgc tctaccttcg
    59221 ttatatccag gcagattttt taactgactt tcgtttgaaa actggcgttt ttccaaaact
    59281 ggattacaaa taaatatata aaaaaaacaa tgcgtttagc cgccgttaat ttcatgtaag
    59341 tcatgttgtt ccttttattt aaccgcggac tccgcagtta accggaggat atgcatcatg
    59401 aaaaccgtta gggagtccac aacgttgtac aactttctcg gatcgcacaa tccatactgg
    59461 cggttgacgg aaagcagcga tgttttgcgc ttttctacca ccgaaaccac agaacctgat
    59521 cgtacattgc agttatctgc cgaacaggct gctcgcatca gggaaatgac ggtcatcacc
    59581 tccagcctga tgatgagtct gaccgtcgat gaaagcgatc tttctgtgca tctggtagga
    59641 cgaaaaatca ataaacggga atgggctggc aacgcgtctg catggcatga cacaccggcg
    59701 gttgctcgtg atttatcaca cgggctttcc tttgctgagc aggtagtttc tgaagcacat
    59761 tccgcaatag tgattctcga cagccggggg aatatccaac gcttcaatcg gttatgtgaa
    59821 gattacacag ggttgaaaga acacgacgtc attgggcaaa gcgtgtttaa actgtttatg
    59881 agccgtcgtg aagctgcggc atccaggcgc aataaccgtg tattttttcg aagcggcaat
    59941 gcatatgaag tcgaactgtg gataccaaca tgtaaaggcc agcggctgtt tctgtttcgc
    60001 aataaatttg tccacagcgg cagtggcaaa aacgagattt ttttaatctg ttccggcacc
    60061 gacattaccg aagagcgccg cgctcaggag cgactgcgta ttctggcaaa taccgacagt
    60121 atcaccggac tgccgaatcg taacgcaatg caggatttaa tcgatcacgc tattaatcat
    60181 gcagataaca ataaagttgg ggttgtgtat cttgatttgg ataatttcaa aaaggtcaac
    60241 gacgcctatg ggcatttgtt tggtgaccag ttattacgcg acatgtcatt ggctatttta
    60301 agctgtctcg aacatgacca ggtgttggcg cgtccaggtg gggatgagtt tctggtactg
    60361 gcatccaaca cctcacaaag cgcgctggaa gcaatggcat cacgaatttt gacccgctta
    60421 cggctcccct ttcgcattgg tttaattgaa gtttatacca gctgttcagt aggtattgca
    60481 ctctctcccg aacatggttc agacagcacg gctattattc gtcacgccga cacagcaatg
    60541 tacacagcga aggaaggcgg acgaggacaa ttttgcgttt ttaccccaga aatgaatcaa
    60601 cgggtatttg aatatctctg gctggatacc aacttgcgta aagcactgga aaacgatcag
    60661 ttggttattc actatcaacc gaaaatcacc tagcgtggcg aagtgcgcag tctggaagca
    60721 ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac cgttggactt catttcctac
    60781 gccgaagagt cagggctaat tgtgccttta ggccgttggg tgattctcga tgtcgtacgc
    60841 caggtggcaa agtggcggga aaaaggcata aacctgcgag tggcggtaaa tatttctgca
    60901 cgtcagctcg ccgatcaaac cattttcacc gccctgaaac aggttctcca ggaactcaat
    60961 tttgaatact gccctataga tgttgaactg acagagagtt gtctgattga gaatgatgaa
    61021 ctggcactgt ctgttattca acaatttagc caactaggtg cgcaagtgca tctggatgat
    61081 tttggtaccg gctactcttc actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa
    61141 cttgaccagg tttttgttcg agatattcac aaacaacctg tctcgcagtc actggtccgg
    61201 gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga tcgccgaagg tgtagagagt
    61261 gcaaaggaag atgctttttt aaccaagaac gggatcaatg agcggcaagg atttttgttt
    61321 gccaaaccga tgcccgccgt cgccttcgaa cgctggtata aacgctatct gaagcgcgca
    61381 taagtagatg aatatatata cttagcgcta tatcccgtca ttatatatcg tattcttgga
    61441 tgataattag ccgttgctgt taaacacttc tcttttagcg gcaatttttg cattattcat
    61501 tttccccctt ttgctataag gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc
    61561 gaacgtattg atattgtgtt agatatcctt gtggcaggcg attaccactc tgcgatccat
    61621 aatctcgaga tacttaaagc agaactcttg cgccaggttg ctgaatctac gccagacatc
    61681 ccgaaggcac cgtgggagat ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc
    61741 cggcgcgtca gtattttttt atttagtatt ataacgttat aagaattaca gcgatgaatt
    61801 cccgacaaca aactattcta cagatggtca ttgaccaggg tcaggttagc gtaaccgatc
    61861 tggcaaaagc cactggagtt tctgaagtta ccattcgcca ggatctcaac accctcgaaa
    61921 aactgagtta cctccgccgt gcacatggct ttgcagtttc gcttgatagt gatgacgtcg
    61981 aaacccgtat gatgagcaac tatacgctga agcgtgaact cgccgagttt gccgcgtctc
    62041 tggttcaacc gggcgaaacc atctttatcg aaaatggtag cagtaatgcc ctgctcgccc
    62101 ggactctggg cgaacagaag aaaaatgtca ctatcatcac agtcagcagc tacattgcgc
    62161 atttgctaaa agacgcgcct tgtgaagtca ttttgctcgg tggcgtgtac cagaaaaaaa
    62221 gcgaaagtat ggttggccct ttgacacgcc agtgcatcca gcaggtacat ttcagcaaag
    62281 catttattgg tattgatggc tggcaacctg aaactggatt caccggtcgc gatatgatgc
    62341 gtaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga agcgatagtc ctgactgaca
    62401 gctcgaaatt tggtgctgta cattcatact ccatcggtcc cgttgagcga ttcaatcgcg
    62461 tgattaccga ttcgagaata cgcgccagcg atctgatgca tcttgagcac agtaaactca
    62521 ccgttcacgt cgttgacatt taaccatttc ccttctctac ggatgatttg cagtttggca
    62581 aatcatccgc tctaagatga ttcctggttg ataattaaga ctatttacct gttattaaca
    62641 cgcttaagat ataaaattat tatcagcgat ataacaggaa gtcattatca cctgcgtgat
    62701 ataaccctgc gcgcgagcag atttcacgga ataatttcac caggcttatt cttagctatt
    62761 atagttatag agagcttact tccgtgaatc ataaattcag gagagagtat tatgtttgta
    62821 acgagcaaaa aaatgaccgc ggctgttctg gcaattactt tggcaatgtc tctgagtgcc
    62881 tgttctaact ggtctaaacg ggaccgcaac accgcaatcg gcgcgggtgc aggggcatta
    62941 ggcggtgcag tactgaccga tggcagtacg ttgggtacat taggtggtgc agctgtcggt
    63001 ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa atgtcgatta tcgactcgca
    63061 attataatac gcttgattct gttcagtacg aaataactga ataatacaca cagtaaaata
    63121 ttcgaaagcc acggatatat ccgtggcttt ttatgttaac cgcctgcgag ttttaccttc
    63181 atccctttcg cttcaagtag tgattttaat aaatcacgct tatcgccctg gatttcaata
    63241 actccatctt taactgctcc gccgcagccg cattttttct tcagttccgc tgcaagtttt
    63301 gtcagttcgg catcatcgag atcgacaccg gtaatcaggc aaacgccctt acctttacgt
    63361 ccactggtct gacgctgaat acgcaccaca ccgtcgcctt tagggcgtac aggggccgct
    63421 ttgggctcat caatacgtcc ggtttccgtt gagtagacca gacggctgtt ggaatcactc
    63481 atcatgcact ccgctgtaaa gaggcgttga tcgctttcag cgtctgcgct ggatctaccg
    63541 attgcgttac cgggcgacca atcaccatat aatcaacacc agccgacaac gcctgttctg
    63601 gcgtcataat gcggcgctgg tcaccagctt cactcccctg cggacgaatg cccggcgtaa
    63661 ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac agcttcctga gcagaacaca
    63721 ccacaccatc aaggccacat ttttgcgtca gtgccgccag acgttctgca taatctgcag
    63781 gtgacagtgt catgccaaga tcgaccaggt cgctggcttc catgctggtc aacactgtca
    63841 cagcaatcaa aagcggtgca tctttgccaa acggaaccag tgcctcacgc gctgcggtca
    63901 tcatacgcgc cccaccagag gcatgaacat tcaccatcca cacgcctaag tcagctgcag
    63961 cagcgacagc gtgcgcagca gtgttaggaa tatcgtggaa tttcaggtca agaaagatat
    64021 caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc aaacaatgta aacatctctt
    64081 tgccgacctt cagacgacaa tcgcgtgggt cgatcttgtc gacaaaggac agcgcgtcat
    64141 cacgattatg ataatcaagg gcaacaacca caggagaatt cgtaacagcg cgggaagaag
    64201 atgaagcagt taacgtcatg accagacctt cttgatgatg ggcaccggaa gtggcgcgaa
    64261 acaggtaaac ggcgagcatt ctacccgtgg aaataaaaaa ttaacagttg cgatttttcc
    64321 cctggcaggc tttttctgcg ccgtcgttgc cggagcacaa tggaacatca ataactagta
    64381 tgttgtaact aaagtaggat tttttaaaaa ttacaggcca tcaagaccgc gaatcggttt
    64441 aatggttgac caggcccgac aagacggaca atgccagtag agggtgtatg cggtaaaacc
    64501 acatttctgg cagcgataac gaggcttact acgtaccttt tcgccaacca tgtcacgcag
    64561 caccatcagg ctctctttgg cacgcccttc ttccgcttca tttaagtggt aatccattaa
    64621 cttatggaac acacgcatgg tcggatgacg ctgaagctgg cgcgtaatat agacctgtgc
    64681 ggcctcacta ccgtcgcgcg cttcgatgat atcagcaagc atcaattcag catcggcacc
    64741 ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat tcggcagttt tacccaactg
    64801 ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc agttctctgt cctgggatat
    64861 cacgcgttgc aaactttcga cggctttggc gtattctcct ttcgccataa acacgcgtcc
    64921 catcatgatg gatacgcggg cgctgttttt atctgccgcc gccccttttt ttagcaaggt
    64981 catggcacga tcgagatcgt cgctggccat atgctgcagg gctaactcac agtagaaatg
    65041 ggcaatttcg acgcgctgtt tatctttacc cagcttcacc aggcgttcgg caacatcaat
    65101 tgctttctgc cactcactgg tagcctggta gatttgtagc aactgttgca gagcgccaat
    65161 gcggaagtca gtttcatcgg tcagctgatt gaacatgtct tccgcgcggt cgtataaccc
    65221 ggcggccatg taatcacgcc ctagttgttg aatcgccaac agacgctgtt cataggtcag
    65281 cgaggcgctt tccattaggg tctgatggat gcgaatagcg cgatcaactt cgccacgcga
    65341 acggaacagg tttccgagcg taaggtgggc ttcaacggtg cctgtatcct ctttaagcat
    65401 atcgagaaac aggtctaccg ctttatcctg ttgattacta agcaggaagt taacccccgc
    65461 tacgtaatca cgcgacaagc ggttggcttc atcttgcttg ttttgttgcg cacttctgcg
    65521 gcccatatac cagccatagg cagcggctac aggcaaaagc agaaacaaca actccagcat
    65581 agaaagttat tccttcgccg ctgacgagtg cggcactaca gccacgtcag tcgcgggtga
    65641 aagctggttt tccagtcgct ttattttacg ttcagcgcgc gccagggaaa cacgaactcg
    65701 cagccagaac aggccacaaa tcaaccaacc gatagcaaac cccgcagcaa acaataccgc
    65761 cagcaatgtg gaaatacggt actccccttg cgctaacaga taattaaacg tcacctgttg
    65821 atcgttctgc gcacccaacg tgaccgaaat cacgaagatc gctaacacca gtaagaaaat
    65881 gagtaaatat ttcacattac ttcccgttat gtggttagag cgaataaatt atggtcaact
    65941 taccgcaata gccctttaaa gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc
    66001 attgttgatg atttaagggc taaattcgga aaatcaacca gcgttaactt tcttgttctc
    66061 gttgcgctat ttcgcgattt tcttcctcag gtggtgttaa tggcccacaa attcgttgcg
    66121 caagccacgt tgccaccgcc accagcgccc acgaaatcaa cgtagctact accagatcgc
    66181 gtggccaatg catcccgagc agcaggcggc ttcccatgac tcccgttgcc cagaccagca
    66241 agatagcaat ggttaacgtt cgccgacgcg gccacagcaa accaacggcc agcagtgccc
    66301 aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa ccccgtctct ttctgccaat
    66361 gtgaacgcaa atattgtggg atatttttct cttcagccaa ctgttctttc actagatttc
    66421 cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg aatatgatgt gttttttcca
    66481 gccagataac aaaaggtcgt ggttcctgga ctttgtcttt gatccaggat ttaacgcctt
    66541 gtcccacaag gattgcggcc gccagaatgg caaataatac aaaggcagcc ttaatgcgaa
    66601 aacgcagaca ccagagaaac cagccgaata aaatcaaatg tgtaatgacg ccccagggct
    66661 gggtgacagt ttcagtaacc caaaaagccg cttttagtag ccaactttgt tctccaggtt
    66721 gccaacgcca gccagaaatc catacggcta ctggcatgac aagcaatagt gcagctccca
    66781 ctgcggtacg tctggcaatc gaacgcatgg cctctccttt ttgataagtc ccacaatcat
    66841 aactgaaaac gccagttcca ggaaaaattg acagatttgt gccattccgt gaacgatcga
    66901 cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa taagccaata cagaaccagc
    66961 attatctgga gaatttcatg cagcttaaac gtgtggcaga agccaaactg ccaaccccat
    67021 ggggcgattt cctgatggtg ggatttgaag aactggcaac cggacacgat catgtcgcgc
    67081 tagtctatgg cgatatttcc gggcataccc cggtacttgc gcgcgtccat tccgaatgtc
    67141 tgaccggtga cgccctgttc agcttgcgct gcgattgtgg cttccagctc gaagcggcat
    67201 tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta
    67261 acattggtct gctgaataaa atccgcgctt acgcactgca ggatcaaggt tacgataccg
    67321 tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg
    67381 atatgttcaa actccttggc gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag
    67441 tcgaaattct gaccgaagca gggattaata ttgttgaacg cgtaccattg attgtaggtc
    67501 gtaaccccaa taacgaacat tatctcgata ccaaagccga gaaaatgggc catttgctga
    67561 acaaataacc ctcttgcatt gtgtaattaa tttgcttgcc ggaagcaaaa taaccggcaa
    67621 gcaaatactt gttacttcaa catattacga atgacataat gcaaaatgcc gtcgttctgg
    67681 tagtaggtca actccgtcgc ggtgtcgata cgacaacggc agggtacaac ctcctggcta
    67741 ccatccgcgc gcgtaagcgt caccggaacc gtcgcaccgg gttgtagatt ttgcagatcg
    67801 ccaatatcaa tcttctcttc cccggttagt cctaacgttt tacgcgttac gccttgcgga
    67861 aattccagcg gcaggatgcc catgccaatt aaattcgaac ggtgaattcg ttcaaacgat
    67921 tcggcaatca ccacacgaat tccaagcaga cgaggacctt ttgccgccca gtcacggctt
    67981 gagcctgatc catactcttt cccggcaatc accgccagcg gcgtttgttc cttcttatag
    68041 cgcatcgcag catcataaat agagactacg tcgctgtcag gtaaatgccg cgtcatcccc
    68101 ccttcaacgc caggcaccat ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc
    68161 atcacttcat ggttaccacg ccgcgaaccg taggagttaa agtcttttcg ctcaacaccc
    68221 cgaccttgta gatatcgacc cgctgggctg tcgggcttaa tactgcccgc cggagagata
    68281 tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc gcgcaccgtg aatatcttcc
    68341 actggtgctg gtgttgcctg catttcatca aagaaaggcg ataagcgaat ataggttgag
    68401 tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa tttccttcca ctctgctgtg
    68461 ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg tggagacttg ttctaccgca
    68521 cgggcaattt cttgtgccga tggccagata tctttcagat aaaccgggtc gcctttacga
    68581 tcatgaccaa taggctcagc agccaggttg atattcatat ttcccgccag cgcataggca
    68641 accaccagcg gcggcgaggc cagccagtta gttttaacca gcggatggat acggccttca
    68701 aagttacggt tgccggacag caccgcaccg acggttaaat cgcctttttt gattgccgtt
    68761 tcgataggat cgggcagcgg cccagagtta ccaatacagg tggtacaacc gtatcccaca
    68821 aggttaaacc ccagttcgtc gagatacggt gtcagtttcg cttttgccag ataatcagaa
    68881 acgactttcg aacccggtgc cagcgacgct ttgacccacg gttgccgctt gaggcccaga
    68941 gttacggctt ttttcgccag caagcctgcg gccatcagca cacttgggtt agaggtgttg
    69001 gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag gtaactgata ctgatgtccg
    69061 ttcataacat aatcgaccgg ctggcgatct ttatgcgtgg cattcacttc cagttcgtta
    69121 ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc gatcctgtgg gcgttttggc
    69181 cctgccaggc tcgcttcaac gtcattcata tccagttcta acgtactggt aaaaatcggt
    69241 tcatcgcccg ggttacgcca catgccctgc gctttggcat atttttcgac caactcaacc
    69301 tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg ttacagcatc gattgggaag
    69361 aagccacagg tggcaccata ttctggcgac atattggcaa tggtggcgcg atccgccagc
    69421 ggtagtgaat ccagaccatc accataaaat tcgacgaatt tccccaccac gccatgtttg
    69481 cgcagcattt gggtaacagt gagaaccagg tctgtggcgg taataccttc acgtaattta
    69541 ccggtaagtt taaagcccac tacgtccggg ataagcat
//
LOCUS       DAZUFB010000011        67549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZUFB010000011 DAZUFB010000000
VERSION     DAZUFB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.011"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..481)
                     /locus_tag="ACHY3M_000863"
     CDS             complement(<1..481)
                     /locus_tag="ACHY3M_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4315830.1"
                     /translation="MKKIVIIGNVASMMLRFRKELIMNLVRQGDNVYCLANDFSTEDL
                     KVLSSWGVKGVKFSLNSKGINPFKDIIAVYELTKILKDISPDIVFSYFVKPVIFGTIA
                     SKLSKVPRIVGMIEGLGNAFTYYKGKQTTKTKMIKWIQILLYKLALPMLDDLILLNHD
                     "
     gene            complement(1001..1894)
                     /gene="galF"
                     /locus_tag="ACHY3M_000864"
     CDS             complement(1001..1894)
                     /gene="galF"
                     /locus_tag="ACHY3M_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HFS4315831.1"
                     /translation="MINLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEIVLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQAQLLGLGHSILCARPIVGDNPFVVVLPDVVLDTASADPLRYNLAAMVARFNETGRS
                     QVLAKRMDDDLSEYSVIQTKEPLDNEGKVSRIVEFIEKPDQPQTLDSDLMAVGRYVLS
                     ADIWAELEKTAPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKSIKKLLSE"
     gene            complement(2000..3228)
                     /locus_tag="ACHY3M_000865"
     CDS             complement(join(2000..2913,2913..3228))
                     /locus_tag="ACHY3M_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFS4315832.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARVWGVSLVSRCLRVSRAQLHVILRRTDDWMDSRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            complement(3365..4237)
                     /locus_tag="ACHY3M_000866"
                     /pseudo
     CDS             complement(3365..4237)
                     /locus_tag="ACHY3M_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(4305..4562)
                     /locus_tag="ACHY3M_000867"
                     /pseudo
     CDS             complement(4305..4562)
                     /locus_tag="ACHY3M_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4635..5192)
                     /locus_tag="ACHY3M_000868"
                     /pseudo
     CDS             complement(4635..5192)
                     /locus_tag="ACHY3M_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(5217..5771)
                     /gene="wcaF"
                     /locus_tag="ACHY3M_000869"
     CDS             complement(5217..5771)
                     /gene="wcaF"
                     /locus_tag="ACHY3M_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFS4315833.1"
                     /translation="MQDLSGFKVPGGFRGGGAIKVQLWWAVQATLFRWSPQVMYRWRA
                     FLLRMFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDAVLYTLGDITIGAHAVISQ
                     KSYLCTGSHDYASQHFDINATPIVVGAKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVTRERVEKVTP"
     gene            complement(5781..6527)
                     /gene="wcaE"
                     /locus_tag="ACHY3M_000870"
     CDS             complement(5781..6527)
                     /gene="wcaE"
                     /locus_tag="ACHY3M_000870"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFS4315834.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAGDISFEWIVVDGGSS
                     DGTSAFLEGLSGEHRLRFVSEPDNGIYDAMNKGIAMARGRFALFLNSGDILHADAAHF
                     IRQLKMQSDQTMVIGDALLDFGNGNKIKRRAKPGWYIYHSLPASHQAIFIPLSGLNVW
                     HYDLQYKISSDYALAAQLYKSGYAFKKLSGLVSEFSMGGVSTTNNLELCRDAKKVQRQ
                     ILHMPNFWTGLSEYLRLRTTAKTKTLYGKT"
     gene            complement(6533..7750)
                     /gene="wcaD"
                     /locus_tag="ACHY3M_000871"
     CDS             complement(6533..7750)
                     /gene="wcaD"
                     /locus_tag="ACHY3M_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFS4315835.1"
                     /translation="MSRSIRICSYLVLPLLYLLVNVKLAQLGESFPITIVTFLPALLL
                     LFVERINIKKLMIALGVGGGLTAFNYLFGQSLDASKYVTSAMLFVYTVVIIGMVWSIR
                     FKTISPHNYIKILRFFWLVVGLVVGLAAVEMAQIILSGGSSLMEVISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKDAIKKKLPLAIISLSVFLVGVIFAFPYIATRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLLNVDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVILALWYMGKVMKMMINAFGDNQNYRVQLYLFTPLSLFFTGSVFSPEYAFLIV
                     CPFILRKALNIAR"
     gene            complement(7725..8947)
                     /gene="wcaC"
                     /locus_tag="ACHY3M_000872"
                     /pseudo
     CDS             complement(7725..8947)
                     /gene="wcaC"
                     /locus_tag="ACHY3M_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
     gene            complement(8947..9432)
                     /gene="wcaB"
                     /locus_tag="ACHY3M_000873"
     CDS             complement(8947..9432)
                     /gene="wcaB"
                     /locus_tag="ACHY3M_000873"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFS4315836.1"
                     /translation="MLEDLRANGWSLRPCCMVAAYRIAHFCSVWRKKNIINNLWAAPV
                     LVMYRLITECLFGYEIQAAASIGRRFTIHHGYGVVINKHVVAGDDFTLRHGVTIGNLG
                     ANDPSCPVIGNSVELGANVTLLGDVAIGDNVTIGAGSVVVDSVPDNTLVVGEKARVKV
                     I"
     gene            complement(9435..10277)
                     /gene="wcaA"
                     /locus_tag="ACHY3M_000874"
     CDS             complement(9435..10277)
                     /gene="wcaA"
                     /locus_tag="ACHY3M_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFS4315837.1"
                     /translation="MTTENPLISIYMPTWNRQQLAIRAIKSVLRQDYPHWEMIIVDDC
                     SSSYEQLQQFVEELNDPRVSYTHNSTNSGACAVRNQAIMQANGQYITGIDDDDEWTPN
                     RLSVFLQYRHQLVTRAFLYANDYVCEGEVYSQPTSLPLYPKSVYSRRRFYKRNIIGNQ
                     VFTWAWRFKACLFDTTLKAAQDYDIFLRMVVAYGKPWKVKEATQILHVNHGEMRITSS
                     PNKFSGYFQFYRKHKGKFDRASKKYQLFTLYQIRNKRMNWRTLLTLLSVRNSKRLADG
                     LRGR"
     gene            complement(10350..12512)
                     /gene="wzc"
                     /locus_tag="ACHY3M_000875"
     CDS             complement(10350..12512)
                     /gene="wzc"
                     /locus_tag="ACHY3M_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFS4315838.1"
                     /translation="MTEKVKQSAAPVTGNDEIDIGRLVATVVEARWWVLGITTAFALC
                     AAVYTLFATPIYSADALVQIEQNTGNSLVQDIGSALSNKPPASEAEIQLIQSRLVLGK
                     TVDDLNLDIAVTKDTFPIFGAGWDRLMGRQNETVNVTTFTLPKAMNEQTFTLNVLNDK
                     SYQLVSDGGFSARGQVGKVLEHDGVTMLVEAIHASPESQFTVSKFSTLGMINTLQNNL
                     MVTETGKDTGVLSLTFTGEDREQIRQILDSITRNYLQQNVERKSEEAAKSLAFLAKQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLF
                     TKAHPAYRTLLEKRQALEDEKSKLNGRVTAMLKTQQEIVRLTRDVESGQQVYMQLLNK
                     QQELKITEASTVGDVRIVDPAIAQLGVLKPKTALIILGSIILCLMLSVVGVLLRSLFN
                     RGIESPLALEEHGISVYASIPLSEWQKARDNVQTIKGVKRYKQSQLLAVGNPTDLAIE
                     AVRSLRTSLHFAMMQARNNVLMLTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGKGDISSSAKLTSIPHFDLIPRGQVPPNPSELLMSER
                     FGELIAWASSHYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIQVKGVILNSIFRRASGYQDYGYYEYEYTSDAK"
     gene            complement(12509..12958)
                     /gene="wzb"
                     /locus_tag="ACHY3M_000876"
     CDS             complement(12509..12958)
                     /gene="wzb"
                     /locus_tag="ACHY3M_000876"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFS4315839.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRDAFDAVYALLERSARQWAQALNAEQGKQ"
     gene            complement(12964..14103)
                     /gene="wza"
                     /locus_tag="ACHY3M_000877"
                     /pseudo
     CDS             complement(12964..14103)
                     /gene="wza"
                     /locus_tag="ACHY3M_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
     gene            14412..14561
                     /locus_tag="ACHY3M_000878"
     CDS             14412..14561
                     /locus_tag="ACHY3M_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315840.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            14762..16345
                     /gene="yegH"
                     /locus_tag="ACHY3M_000879"
     CDS             14762..16345
                     /gene="yegH"
                     /locus_tag="ACHY3M_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS4315841.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLTLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(16619..18472)
                     /gene="asmA"
                     /locus_tag="ACHY3M_000880"
     CDS             complement(16619..18472)
                     /gene="asmA"
                     /locus_tag="ACHY3M_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFS4315842.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDITSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(18494..19075)
                     /gene="dcd"
                     /locus_tag="ACHY3M_000881"
     CDS             complement(18494..19075)
                     /gene="dcd"
                     /locus_tag="ACHY3M_000881"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFS4315843.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(19167..19808)
                     /gene="udk"
                     /locus_tag="ACHY3M_000882"
     CDS             complement(19167..19808)
                     /gene="udk"
                     /locus_tag="ACHY3M_000882"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4315844.1"
                     /translation="MTEQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERIKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            20126..23443
                     /gene="dgcE"
                     /locus_tag="ACHY3M_000883"
     CDS             20126..23443
                     /gene="dgcE"
                     /locus_tag="ACHY3M_000883"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4315845.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESEPRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNIESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(23552..24400)
                     /gene="alkA"
                     /locus_tag="ACHY3M_000884"
     CDS             complement(23552..24400)
                     /gene="alkA"
                     /locus_tag="ACHY3M_000884"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFS4315846.1"
                     /translation="MYTLNWQSPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAVPDIARHTLHINLSVGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPSSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGSW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            24534..25886
                     /gene="yegD"
                     /locus_tag="ACHY3M_000885"
     CDS             24534..25886
                     /gene="yegD"
                     /locus_tag="ACHY3M_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4315847.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPETITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(25899..27839)
                     /gene="yegI"
                     /locus_tag="ACHY3M_000886"
     CDS             complement(25899..27839)
                     /gene="yegI"
                     /locus_tag="ACHY3M_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFS4315848.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYESDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKVAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(27836..28597)
                     /gene="pphC"
                     /locus_tag="ACHY3M_000887"
     CDS             complement(27836..28597)
                     /gene="pphC"
                     /locus_tag="ACHY3M_000887"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFS4315849.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFF
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(28594..29253)
                     /gene="yegL"
                     /locus_tag="ACHY3M_000888"
     CDS             complement(28594..29253)
                     /gene="yegL"
                     /locus_tag="ACHY3M_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS4315850.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSCSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(29474..29533)
                     /gene="ibsA"
                     /locus_tag="ACHY3M_000889"
     CDS             complement(29474..29533)
                     /gene="ibsA"
                     /locus_tag="ACHY3M_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFS4315851.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(29807..29863)
                     /locus_tag="ACHY3M_000890"
     CDS             complement(29807..29863)
                     /locus_tag="ACHY3M_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4315852.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(30135..30191)
                     /locus_tag="ACHY3M_000891"
     CDS             complement(30135..30191)
                     /locus_tag="ACHY3M_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4315853.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            30470..31717
                     /gene="mdtA"
                     /locus_tag="ACHY3M_000892"
     CDS             30470..31717
                     /gene="mdtA"
                     /locus_tag="ACHY3M_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFS4315854.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            31717..34839
                     /gene="mdtB"
                     /locus_tag="ACHY3M_000893"
     CDS             31717..34839
                     /gene="mdtB"
                     /locus_tag="ACHY3M_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFS4315855.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            34840..37917
                     /gene="mdtC"
                     /locus_tag="ACHY3M_000894"
     CDS             34840..37917
                     /gene="mdtC"
                     /locus_tag="ACHY3M_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFS4315856.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            37918..39315
                     /gene="mdtD"
                     /locus_tag="ACHY3M_000895"
     CDS             37918..39315
                     /gene="mdtD"
                     /locus_tag="ACHY3M_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4315857.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIFARVPND
                     THQNVAISRRKRSAQ"
     gene            39312..40715
                     /gene="baeS"
                     /locus_tag="ACHY3M_000896"
     CDS             39312..40715
                     /gene="baeS"
                     /locus_tag="ACHY3M_000896"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFS4315858.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            40712..41434
                     /gene="baeR"
                     /locus_tag="ACHY3M_000897"
     CDS             40712..41434
                     /gene="baeR"
                     /locus_tag="ACHY3M_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFS4315859.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            41625..41957
                     /gene="yegP"
                     /locus_tag="ACHY3M_000898"
     CDS             41625..41957
                     /gene="yegP"
                     /locus_tag="ACHY3M_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFS4315860.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            42166..42462
                     /locus_tag="ACHY3M_000899"
     CDS             42166..42462
                     /locus_tag="ACHY3M_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS4315861.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            42464..42760
                     /locus_tag="ACHY3M_000900"
     CDS             42464..42760
                     /locus_tag="ACHY3M_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4315862.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            42863..44224
                     /gene="yegQ"
                     /locus_tag="ACHY3M_000901"
     CDS             42863..44224
                     /gene="yegQ"
                     /locus_tag="ACHY3M_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFS4315863.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(44554..44871)
                     /gene="yegR"
                     /locus_tag="ACHY3M_000902"
     CDS             complement(44554..44871)
                     /gene="yegR"
                     /locus_tag="ACHY3M_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFS4315864.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            45286..46185
                     /gene="yegS"
                     /locus_tag="ACHY3M_000903"
     CDS             45286..46185
                     /gene="yegS"
                     /locus_tag="ACHY3M_000903"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFS4315865.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(46267..47040)
                     /locus_tag="ACHY3M_000904"
     CDS             complement(46267..47040)
                     /locus_tag="ACHY3M_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4315866.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(47146..48186)
                     /gene="gatD"
                     /locus_tag="ACHY3M_000905"
     CDS             complement(47146..48186)
                     /gene="gatD"
                     /locus_tag="ACHY3M_000905"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4315867.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(48234..49589)
                     /locus_tag="ACHY3M_000906"
     CDS             complement(48234..49589)
                     /locus_tag="ACHY3M_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS4315868.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(49593..49877)
                     /gene="gatB"
                     /locus_tag="ACHY3M_000907"
     CDS             complement(49593..49877)
                     /gene="gatB"
                     /locus_tag="ACHY3M_000907"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFS4315869.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(49908..50360)
                     /gene="gatA"
                     /locus_tag="ACHY3M_000908"
     CDS             complement(49908..50360)
                     /gene="gatA"
                     /locus_tag="ACHY3M_000908"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFS4315870.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(50370..51632)
                     /gene="gatZ"
                     /locus_tag="ACHY3M_000909"
     CDS             complement(50370..51632)
                     /gene="gatZ"
                     /locus_tag="ACHY3M_000909"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFS4315871.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(51661..52515)
                     /gene="gatY"
                     /locus_tag="ACHY3M_000910"
     CDS             complement(51661..52515)
                     /gene="gatY"
                     /locus_tag="ACHY3M_000910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFS4315872.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTESLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(52825..53877)
                     /gene="fbaB"
                     /locus_tag="ACHY3M_000911"
     CDS             complement(52825..53877)
                     /gene="fbaB"
                     /locus_tag="ACHY3M_000911"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFS4315873.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            54134..55411
                     /gene="yegT"
                     /locus_tag="ACHY3M_000912"
     CDS             54134..55411
                     /gene="yegT"
                     /locus_tag="ACHY3M_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFS4315874.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            55408..56412
                     /gene="yegU"
                     /locus_tag="ACHY3M_000913"
     CDS             55408..56412
                     /gene="yegU"
                     /locus_tag="ACHY3M_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS4315875.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            56409..57374
                     /gene="yegV"
                     /locus_tag="ACHY3M_000914"
     CDS             56409..57374
                     /gene="yegV"
                     /locus_tag="ACHY3M_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS4315876.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(57348..58094)
                     /gene="yegW"
                     /locus_tag="ACHY3M_000915"
     CDS             complement(57348..58094)
                     /gene="yegW"
                     /locus_tag="ACHY3M_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4315877.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(58146..58964)
                     /gene="yegX"
                     /locus_tag="ACHY3M_000916"
     CDS             complement(58146..58964)
                     /gene="yegX"
                     /locus_tag="ACHY3M_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFS4315878.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVIYHTNLAGYVNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(59029..59829)
                     /gene="thiD"
                     /locus_tag="ACHY3M_000917"
     CDS             complement(59029..59829)
                     /gene="thiD"
                     /locus_tag="ACHY3M_000917"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4315879.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(59826..60614)
                     /gene="thiM"
                     /locus_tag="ACHY3M_000918"
     CDS             complement(59826..60614)
                     /gene="thiM"
                     /locus_tag="ACHY3M_000918"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFS4315880.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(60649..60745)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(60837..61109)
                     /gene="rcnR"
                     /locus_tag="ACHY3M_000919"
     CDS             complement(60837..61109)
                     /gene="rcnR"
                     /locus_tag="ACHY3M_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFS4315881.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            61229..62053
                     /gene="rcnA"
                     /locus_tag="ACHY3M_000920"
     CDS             61229..62053
                     /gene="rcnA"
                     /locus_tag="ACHY3M_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFS4315882.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            62272..62610
                     /gene="rcnB"
                     /locus_tag="ACHY3M_000921"
     CDS             62272..62610
                     /gene="rcnB"
                     /locus_tag="ACHY3M_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFS4315883.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(62692..63726)
                     /gene="yehA"
                     /locus_tag="ACHY3M_000922"
     CDS             complement(62692..63726)
                     /gene="yehA"
                     /locus_tag="ACHY3M_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFS4315884.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(63740..66220)
                     /gene="yehB"
                     /locus_tag="ACHY3M_000923"
     CDS             complement(63740..66220)
                     /gene="yehB"
                     /locus_tag="ACHY3M_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS4315885.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(66236..66910)
                     /gene="yehC"
                     /locus_tag="ACHY3M_000924"
     CDS             complement(66236..66910)
                     /gene="yehC"
                     /locus_tag="ACHY3M_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4315886.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(66991..67532)
                     /gene="yehD"
                     /locus_tag="ACHY3M_000925"
                     /pseudo
     CDS             complement(66991..67532)
                     /gene="yehD"
                     /locus_tag="ACHY3M_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT        16388 a        17623 c        16543 g        16995 t
ORIGIN      
        1 catcatgatt taatagaatc aaatcatcaa gcatcggtaa tgctaactta tataaaagaa
       61 tttgtatcca ctttatcatt ttagtttttg tggtctgctt tcccttataa taagtgaagg
      121 cattacctag accttcaatc attccaacaa tccttggcac ttttgacaac tttgaagcaa
      181 tagttccaaa tattactggc tttacaaaat atgaaaatac aatatctggg gaaatatcct
      241 taagaatttt tgttagttca taaacagcaa ttatatcctt aaaaggatta ataccctttg
      301 agttaagaga gaatttaacc cccttaacgc cccatgacga aagtacttta agatcttcag
      361 tggaaaaatc atttgctaga caatatacat tatcaccttg cctcactaaa ttcatgatta
      421 attctttcct gaaccttaac atcattgacg ctacattgcc tatgatcact atttttttca
      481 tatcacattc taaaagtaaa taatatacat tgatatgtat gcagcatagt aacatcaact
      541 gccatctaaa tcggaaaatg gataatttga aaagtactac taccaaaacc aatttccctg
      601 agcgactaca aacactcttt tacctaatgg aaaagaagtt taatccgatt gactttattt
      661 gctataagcg gcaagaataa atgtatacgt taaaaattaa ttaggcaagc taccgcccct
      721 ggcttaacag ctaccagtgc actaactaaa tgccaaagat gtgagcagtt ccgcgagatc
      781 cagcaaaact ctctaaaact cacgctaatt gtcttaccaa atgcgaccag aaacaagaaa
      841 aaatccaaca aaaagcagat taaacttggt aggttaactt gttattattg gtcatttttc
      901 ataaaaaacg gcagtaacat tccattgctt aaagcataga agcctggatg ttaactgccg
      961 ctattttttc tggcttatcg tcacaaagac gtgtaaatct ctactcactc agtagcttct
     1021 tgatgctctt acggaacttc gccccttctt taaggttgcg cagcccatac ttaacgaatg
     1081 cctgcatata gcccatcttc ttaccgcagt cgtagctgtc gccggtcatc agcatggcat
     1141 caacagactg ttttttagcc aactctgcaa tagcatcagt cagttgaata cgtccccagg
     1201 cacctggagc agttttttcc aactcagccc agatatcagc agaaagcacg tagcgcccta
     1261 cggccatcag atcggaatca agcgtttgtg gctgatcggg tttttcaatg aattcaacga
     1321 tacggctgac tttgccttcg ttgtccaaag gctctttggt ctgaataaca gagtattcgg
     1381 acagatcatc atccatgcgt ttagccagca cctgactgcg cccggtttca ttaaaacggg
     1441 caaccatggc agccaggtta taacgcaacg ggtccgcact cgctgtatcc agaacgacgt
     1501 caggcaatac gacgacaaac gggttatccc caacaatcgg gcgagcacaa agaatggaat
     1561 gacccagacc cagtaactga gcctgacgaa cgttcataat agtcacgcca ggaggacaga
     1621 tagactgtac ctcggctaaa agctggcgct taacacgctg ctcaaggagt gattcgagtt
     1681 cgtaagaggt gtcgaagtgg ttctcaaccg cgttcttaga cgcatgagtc accagaacga
     1741 tttctttgat accggcagct acaatttcgt cgacaatgta ctgaatcatc ggcttgtcta
     1801 cgatcggcag catctcttta gggattgcct tcgtggcagg caacatgtgc atccccagac
     1861 ctgctaccgg aataactgct ttcaaattaa tcattaagtc attcacctat tcaattggtg
     1921 ctgaattata gctttttagc ttgttttagc cagtatgaat tacattactc cgatgccgcg
     1981 tatgttactg gatttgcccc tatatttcca gacatctgtt atcacttaac ccattacaag
     2041 cccgctgccg cagatattcc cgtggcgagc gataacccag cgcactatgc ggatgccatt
     2101 cgttataatg ctcgaacgcc tctgcaaggt tctttgctgc cgttaacccg tctggtttgg
     2161 gcatgatact gatgtagtca cgctttatcg ttttcacgaa gctctctgct attccgttac
     2221 tctccggact ccgcaccgcc gtgttcttcg gttcaagtcc caacatccgg gcgaactggc
     2281 gtgtttcatt agcccggtag catgaaccat tatccgtcag ccactccact ggagacgacg
     2341 gaagatcgtt gccgaagcgg cgttccaccg ctcccagcat gacgtcctgt actgtttcac
     2401 tgttgaagcc gccggtagtc accgcccagt gcagtgcctc acgatcacag cagtccagcg
     2461 cgaacgtgac acgcagtctc tctccgttat cacagcagaa ctcgaacccg tcagagcacc
     2521 atcgctgatt gctttctttc acggccactc tgcctgtatg tgcccgtttc gatggcggta
     2581 cagcaggttt tcgctcaagc aacagcgcat tctggcgcat gatccggtaa acacgtttgg
     2641 cattgatcgc aggcatacca tcaagttctg cctgtctgcg aagcagcgcc catacccgac
     2701 gataaccata cgtgggcagc tctccgataa catggtgtat acggagaagc acatccgtat
     2761 catcagtgtg acgactgcgg cggctatcca tccagtcatc ggttcgtctg agaatgacgt
     2821 gcaactgcgc acgcgacacc cggagacaac ggctgactaa gcttactccc catacccggg
     2881 caataagggc gcgtgcgcta tccacttttt tgcccgtcca tattcaacgg cttctttgag
     2941 gagttcattt tccatcgttt tcttgccgag caggcgctgg agttctttaa tctgcttcat
     3001 ggcggcagca agttcagagg caggaacaac ctgttctccg gcggcgacag cggtaagact
     3061 tccttcctgg tattgcttac gccagagaaa taactggctg gctgctacac catgttgccg
     3121 ggcaacgaga gagaccgtca tccccggttc aaagctctgc tgaacaattg cgatcttttc
     3181 ctgtgtggta cgccgtctgc gtttctccgg ccctaagaca tcaatcatct gttctccaat
     3241 gactagtcta aaaactagta ttaagactat cacttattta agtgatactg gttgtctgga
     3301 gattcagggg gccagtctag ttacgccgct taactgcgga atgcagtaat cttaatccca
     3361 ggcgggtcag tgattttctg agttctgacg gtaaactcag gaatttgtcg gtaagagcct
     3421 gatttactga gacagaatct ttgcccctga gtctgaggat gatcgtataa cgtgattttc
     3481 ggtctacaag tgtggctata tgagagtttt ttgtacctga gactaaatcg ccctcccaat
     3541 gccccagaga gcgtctgtta tcgatatttc gggaacgttc gtgaattggt gttccgttca
     3601 ctatgttaat cgtacctctt tcgcctttgc gggtatgacg cctgccatgg cgaaggctat
     3661 gcgaccgtcg cagatgctgt atattcaggt ggtgtagcgc ttcacggcta cgaaagtaca
     3721 gcgttttata aattgtctca ggtgatattc gcagcgtttt ttgacgtggt tttgttcgcc
     3781 ttaaccatcc tgatatttgc tctggagacc atttcatctc cagcttttcc agaacaagct
     3841 ttcgcaatgg taaattttga tccagtaagc acggttttgg ccttttcgcc attctgttgg
     3901 ctcggttatt agcatcaaca gctttgtaat agcgtctgcc ccgattacgc tgaacttcac
     3961 gtgagatcgt cgaaggactg cgattcagcg cagtagctat cgcacgaatg ctcattttgg
     4021 ctgacaaacc agctcgtatc tcctcgcgct cagacagtgt caggtgagct acagcccgct
     4081 tacgctcatg gggttttatg ccgccagtat cccttaacat agtgaagatc gttccgggtt
     4141 ttgaacccag gatattcgct atttcactga agcctgttcc gttcttccat agttcaaaaa
     4201 cagaggcttt ttcctctgct gtaaatgttc gtctcattca aaaaacctcc gcaaccccat
     4261 gttttcacat aactgttgcg ttgaccaatt gaatctacag cggctttttt taatatttca
     4321 ttctccattt caatgcgttg tagctttttc ctcagcttac gtatttcgat ttgttctggt
     4381 gttatcggag aggcttttgg tgttttgccc tgacgctcat cacgcagttg tttgacccat
     4441 cttgtcattg tggaaaggcc aacatccata gctttggcgg catctgccac cgtgtatttc
     4501 tggtcaacaa ccagttgagc ggattcgctt ttaaactctg cgctaaaatt tctttttttc
     4561 attggagcac ctgtgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattc
     4621 agtgtgccac ttcagttgaa cagaatgccg ttacaggcat acatcccgta agactcgcgg
     4681 tagttgacgg tgatccagta ggcgtacagc ttagctaccg catagggcga gcgcgggtag
     4741 aacggcgtgg tctctttctg cgggatttcc tgtaccaggc cgtacagttc tgaggtggag
     4801 gcctgataga aacgggtctt cttttccaga ccgaggaagc ggatagcctc cagcaaacgc
     4861 aacgtaccaa ttgcatcgac gtccgcggtg tattccggtg agtcaaacga caccgcgacg
     4921 tgactcatgg cgcccaggtt atagacctca tccggttgga cctcttttaa aatacgggtc
     4981 agattggatg aatccgtcaa atcaccatag tgcagatgaa atttgggatt gcctgaatgc
     5041 ggatcctggt agatgtggtc cacacgttcg gtgttgaatg atgaagcgcg acgcttaata
     5101 ccgtgtactt cgtatccttt ttccagcagg agttctgcca ggtaagaacc atcctgcccg
     5161 gttacgccag taatgagagc gacttttgac atgttataac ctcttcagtg atcccctcag
     5221 ggagtaactt tctcgacgcg ttcgcgcgtg actactgcgg gatttccccg acaaacaaca
     5281 tttgctggca gcgatttaaa tacgctgctt cgtgctccaa cgacggtgcc atcaccaatc
     5341 gtcacgccag gcgcgacaaa gacatctgtc gccaaccagc atttagcgcc aaccacaatc
     5401 ggcgtggcgt taatatcaaa atgttgactg gcataatcat gactcccggt gcataaataa
     5461 cttttttgcg aaataaccgc atgggcgcca atcgtaatat cacctaacgt atataaaacc
     5521 gcgtcgtcac ccacccaggc ataatctccc agggttaatt tccacgggta agtaattttt
     5581 actgaaggcc gaatgactac gttttttcct atttttgcac caaacattcg taataaaaac
     5641 gcacgccagc gatacatgac ctgcggtgac cagcgaaata atgttgcttg caccgcccac
     5701 cataattgca ctttaatagc accaccgccg cgaaaacctc caggcacttt aaatccgctt
     5761 aagtcctgca taatatgccc ttacgttttc ccatataagg tttttgtttt agcggttgtg
     5821 cgcagacgta aatattctga caaaccggtc caaaaatttg gcatgtgtaa tatctggcgc
     5881 tgtacttttt tggcatcccg acacaattcc agattattcg tcgttgacac tccgcccata
     5941 gaaaactccg ataccaaccc gctcaacttt ttaaaagcgt aaccggactt gtataattgg
     6001 gctgccaacg cgtaatcaga cgatatttta tattgcaaat cataatgcca gacattcagt
     6061 cctgagagcg gaatgaatat cgcctgatga ctagcgggca ggctgtgata aatataccaa
     6121 cccggttttg cccggcgctt aattttgttg ccattgccga aatccagcaa cgcatcgcca
     6181 atgaccatgg tttggtcgct ctgcattttt agctggcgga taaaatgtgc cgcgtccgca
     6241 tgcaaaatat ccccggagtt gaggaacagc gcgaaccgcc cgcgcgccat cgcgatgcct
     6301 ttgttcatcg catcatagat gccgttatcc ggctcgctga caaaccgtaa ccggtgttcc
     6361 ccactaagcc cttcaagaaa cgccgacgta ccatccgagg agccgccatc aacaacaatc
     6421 cactcaaaac tgatatcacc cgcttgcgcc agatgtgcca gcgatgcgtg agttttgacg
     6481 atcccttcca gattgcgaaa ggcgacggta atgatactga gcagcatgtg agttacctcg
     6541 cgatattcag cgccttgcgc aatataaacg gacacacaat taaaaatgcg tattccggac
     6601 taaatactga acctgtaaaa aacagtgaca acggtgtaaa aagataaagc tgcacccgat
     6661 aattctgatt atcgccaaac gcattgatca tcattttcat gaccttcccc atgtaccaca
     6721 gggctaatat caccgcgaac catgaaaaat aaataatcag cagatacaaa ccattgtcta
     6781 ttgttttacc gacatctgca ccgttaaaaa ttccgaatga cgcaacgtat tcataaagtg
     6841 agccaaatct cactacgcca tcaacgttca gtaacgaata accgaccatc actaatggcc
     6901 ctacgatacg ataatacgat gacgaacctt cagttcctaa atcaccaagc cgcgttgcga
     6961 tgtacggaaa agcaaatatc accccaacta aaaacacgct taatgagatt atcgccagcg
     7021 gcagcttctt ttttatcgca tccttattca ggtactggaa cgcccactcc aggagataaa
     7081 acaaaataaa cgtcattacc cctgaaaacg atcctgataa tattatccct gccagaatca
     7141 tagcatcgga cttaggtgtt ttgataccaa actgtttgat gctgagccaa attgagatta
     7201 acgccagagc gaagaatgcc ggttcgaaat agagcgcagt agtacgtttc ccaccaaact
     7261 taataaagtt cagaacatag ctgttactgt aaataagata tttcgaaata acttccatta
     7321 aactgctgcc gccgctgaga attatctgcg ccatttcaac cgcagctaac cccaccacca
     7381 gtcccaccac cagccagaaa aaacgcaaaa tcttaatgta gttatgcggt gaaatggttt
     7441 tgaagcgaat actccacacc atgccaatga tcaccacggt ataaacaaac agcatcgctg
     7501 acgttacgta cttactggca tccagtgatt gaccgaacag gtagttaaac gccgtcagcc
     7561 cgccccctac ccccagcgcg atcattaatt ttttgatatt gatgcgctcg acaaacagca
     7621 acagcagagc gggtaagaag gtgacgatag ttatcgggaa gctctcgcca agctgggcga
     7681 gtttgacgtt aaccagcaga taaagcagcg gtagcaccag atagctacag attctgatag
     7741 aacgagacat attcctccag catctgttgc ccactgtatg ccacccgact gcgctgactg
     7801 aatgcggtaa gggacgtgtc aaataccgct tgcgcaatgt ctgatttttt cagctgcacc
     7861 agctgcaata cgtccgcttc gctgaacgtt ttgccgcctg atttggccag cacttcctgc
     7921 gcggcatcgc tgtgcgtggc aattaccggt acgccgatgg aaagcgcttc acacaaaatc
     7981 agcgggtagt tatcgacaca ggagctgaac accagtccat ccatctggtt aagcgcactc
     8041 atcaacgtgc gcttatcgct ctcaaaaccg tggttgatga cgttatctcc cgcaaatggc
     8101 gaaaatttgc cgaaggtatg cagctcaatt ttgtccccaa gcgcgctaat cgctcgcacc
     8161 agttgttgac tggttttgcc gtcatagcgc aggtcatgag ccaccacggc aattttcggc
     8221 cgaccgctct ccgcaggcat cgcgggtagt tcggcaagaa tggcttcggt tgccacatca
     8281 atgccattat tgataacgcg gcagcgtccc acgccataca ggatgttgaa tgcgtcggcc
     8341 acatgttgac tgggcgagat aaactggcag cccagcgcca gcatggcacg gaacatctgc
     8401 cgcttaccgg gcaactgctg atgggcttta tcgactttta ccggcggata gttgctgagc
     8461 gttgggcatt tctgacagcc cgttctccag ccttcgcagc catcggtaaa ggcacagcgc
     8521 ccggtcacgc tccagtgatc gtgcagggtc cagacgaagg tgatatccgg tttatgcgcc
     8581 ttcacttttt ggcaaaaggt caccacctct tccaggttca accagtagct gtgcaacaca
     8641 tggaaatgca gtaccagcgg gccgcaggtg cgggtaaccg tgcgatatag cttgtcgaga
     8701 ttgccgaaca tgtcgcgatt tgcaaaacga aacagcgcaa tgttagccgc cgacgtcagg
     8761 cgcggcgtat atttgataac ctgtgggtat ttcgcatgac tggcgctttt ttaccgcctt
     8821 tgccatagcc atagccatag acaaaccgtg acgacagccc tttttccagc gcgcgaagat
     8881 gcaaatctaa caccacacct gccgccccac cttctgcgag tcgcacatta aactgtaata
     8941 tcttcattat attaccttca cccgtgcctt ctcgcccacg accagcgtat tgtccggaac
     9001 gctatccacc acgacgctcc ccgcgccaat cgtcacgtta tcgccaatag caacgtcgcc
     9061 gagcagagtg acattggccc ccaactcaac gctgttaccg atgaccggac aggatggatc
     9121 gttggcacca agattgccga tagtgacgcc gtggcgtagg gtaaaatcat cccccgccac
     9181 cacgtgtttg ttgataacca ccccataacc atgatggatg gtgaaacgac gaccgattga
     9241 tgcagcagcc tgaatttcat agccaaacag gcattcggtg ataaggcgat acatcaccag
     9301 caccggggca gcccacaggt tgtttatgat atttttttta cgccagacgg agcaaaaatg
     9361 ggcaatccga taggccgcca ccatgcagca ggggcgcaga ctccagccgt tcgcgcgtaa
     9421 atcttccagc ataattagcg tcctcgcagt ccgtcggcca gacgcttact gttacgaaca
     9481 gaaagcagcg tcagcagcgt gcgccagttc atccttttat tgcggatctg atagagggta
     9541 aacagctgat atttcttgct ggcccggtca aatttgcctt tgtgcttgcg gtaaaactga
     9601 aagtaaccgg agaatttatt cggcgatgag gtaatacgca tctcgccgtg attcacatgc
     9661 aggatctgcg tcgcctcttt gaccttccac ggtttgccat acgccaccac catgcgcagg
     9721 aagatgtcgt aatcctgcgc cgctttcagc gtggtatcga acaggcatgc tttaaaccgc
     9781 catgcccagg taaatacctg attaccaatg atgttgcgtt tataaaacct gcggcgcgaa
     9841 tatacggact tcggatagag cggcagactg gtcggctgcg aatacacttc gccctcacag
     9901 acatagtcat tggcatacag aaacgcccgt gtgaccagtt gatgccgata ctgcaggaac
     9961 acgctcaggc ggttcggcgt ccattcatca tcatcatcaa tccccgtaat gtattgcccg
    10021 ttggcctgca taatggcctg attacgcacc gcgcaggccc cggagttggt ggaattatgc
    10081 gtatacgaca cacgcggatc gttgagttcc tcaacaaact gctgcaactg ctcatatgat
    10141 gaagagcaat catccacaat gatcatctcc cagtgagggt agtcctggcg caacaccgat
    10201 ttaatcgccc gaatggccag ctgctggcgg ttccaggttg gcatataaat tgaaattagc
    10261 gggttttctg tcgtcatttt cgtttccgga tagatacgct tttgtaggcc ggataaggcg
    10321 ttttcgccgc atccggcagg gttggtgatt tatttcgcat ccgacgtata ttcgtattcg
    10381 tagtagccgt aatcctgata cccgctggca cggcggaata tcgagttgag gatcacccct
    10441 ttcacctgga tgccattttg ctcgaagcga ctcaggctgg tttccacctc tttcagcgtg
    10501 ttgaccgcat aacgcgccac catcagcgtg gtgccgacat gacggccaac aatcgccgcg
    10561 tcggtcaccg ccagaattgg tggggtgtca atcagcacca ggtcatagtg cgagctggcc
    10621 caggcaatca gttcgccgaa acgctcgctc atcaacagct cggaaggatt gggagggact
    10681 tgaccgcgag ggatcagatc aaaatgcgga atcgatgtca gtttggcgct ggatgagata
    10741 tcccccttac caatcaggat ctcagacagg ccgttgacgt tattggtccc cagcagttcg
    10801 tgggtatagc ctttgcgcat gtcgcaatcg atcaacagca cgcgtttgtt ggtctggctg
    10861 atgactgccg ccaggttggc gcagacaaac gttttaccaa tcgatgggct gacgccggtc
    10921 agcatcaata cgttgttacg cgcctgcatc atggcaaagt gcagactggt acgcaggctg
    10981 cggaccgctt ctatggcgag atccgttgga ttccccacgg ccaacagctg gctttgcttg
    11041 tagcgtttca cacctttaat ggtctgaacg ttatcacgcg ctttttgcca ttcagatagc
    11101 ggaatactag catacacgct gataccatgc tcttccagcg ccagggggct ttcaataccg
    11161 cgattaaaca gagaacgcag cagtacgccc accacagaaa gcatcaaaca aagaatgata
    11221 ctgccgagaa taatcagcgc cgttttcggc ttcagcacgc caagctgggc aatcgcagga
    11281 tcgacaatgc gcacatcgcc aaccgtactg gcctcggtaa tcttcagttc ctgttgcttg
    11341 ttcagcaact gcatatagac ctgctgaccg gactccacat cacgcgtcag acgaacaatt
    11401 tcctgctgcg ttttcagcat cgccgttacg cgcccgttca gcttactttt ctcatcttcc
    11461 agcgcctggc gtttttccag cagcgtgcgg tacgccggat gcgctttggt gaacagcttg
    11521 gaaatctccg cttctttgaa ggtcagttca ttcagctgcg catcgatatt caccatcgaa
    11581 tccagcaccg ctttggcttc cagcggcaaa tccacggagt ctttatcctg acgaaaggcg
    11641 ttcagcttgt tttcagcaac gtcaagccgg ttacgtacct caggcagttg ctttgccaga
    11701 aacgccagac ttttggcggc ctcttcggat ttacgttcca cgttttgttg cagatagttg
    11761 cgggtaatac tgtcgaggat ctggcggatt tgctcgcggt cttcgccggt aaaggtcagg
    11821 ctcaatacgc cggtgtcttt gcccgtttcc gtcaccatca ggttattttg cagggtgttt
    11881 atcatgccca gcgtggagaa tttgctgacg gtaaattggc tctccgggct ggcatgaatc
    11941 gcctcaacga gcatcgtcac gccatcatgt tccaacacct tgccgacttg cccccgggcg
    12001 ctaaaacccc cgtcactcac cagttgatag cttttgtcat tcagcacatt gagcgtgaag
    12061 gtctgttcat tcattgcctt tggcaacgtg aaggtggtca cattaaccgt ttcgttctgg
    12121 cggcccatca gccggtccca gcccgcgcca aaaataggga atgtatcttt ggtaacggcg
    12181 atatcgaggt taagatcgtc taccgttttc cctaatacca gacgcgactg gatgagctga
    12241 atttccgctt ccgaggccgg aggtttgttc gacaacgccg agccaatatc ctgcaccagt
    12301 gaatttccgg tattttgctc aatttgcacc agcgcgtccg cactgtagat gggtgtggcg
    12361 aacagtgtgt agaccgcggc acacaatgca aaagccgtgg taatgcctaa tacccaccag
    12421 cgggcctcca ccaccgttgc aaccagacga ccaatatcga tttcatcatt gccggttacc
    12481 ggcgcagcag actgtttaac tttttctgtc attgttttcc ctgctctgcg ttcagtgcct
    12541 gagcccattg gcgggcagac cgttcaagta atgcgtacac cgcgtcaaac gcatcacggc
    12601 ttttacgata cggatcggga atttcacatt cgttatccca gtgaccaaac agcatcactt
    12661 tgccgcgcat ctcaggtgcc atctcgcata agcgttcgat atggcgcttt tccatggtca
    12721 aaatcaggtc gtagttgcga cacaggctgc ggctgatttg acgggcacag tgaccttcca
    12781 gtgacagttg atgttctgcg gcgacgctga tagcggtagg atcagcgccc ttaccgacca
    12841 gcgcgccgag tccagcggac tccactttca gctccgggtg ataacgttgc agtaagcgtt
    12901 ccgccgtcgg ggaacggcaa atattgccga cacagacaac taagatgttg ttaaacatga
    12961 cgattaccag ttatgaatgt cgctggctgt atccgtcatg taacggacac cgctaatagt
    13021 tggcagcaac tgattgatca gacggttcta gcgggaaacc ggtgcggtgg tgacatacac
    13081 cacgtcataa ggttgcaggc ggaattctgt cgccatcacc agcgacgtgg catcggacat
    13141 atccagctga tagatattgg caatcttgcc gttacgcccg ccctcgcctt tcagcggacg
    13201 aatgacaaag atgccgctgg cgttggaggt ggtcatgtcg atgccttcag cattgcccag
    13261 ggcttcagtc agggtcatgc cgctaaagtc cactttcagg gtgctctgtt ttttaacttc
    13321 acccatcaca aacactttca gatcatcatt acgcggcacg tagagaatat cgccgggata
    13381 aagcagtcgg ttctgattga gatcgccgtt ttgcatcagc gcctgcaaag aaatgcgttc
    13441 ttcacgacca ttgtgtgtta gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc
    13501 tgcggcgttg atggcgtcga gaatggtcag tggtacgttg gtgatcgcct gttgaccgga
    13561 tttattcacc tgaccggaga tatacggttt ttgcgagcgg aaggcggcga tattaacgtc
    13621 cacctgcggg tcagcgatgt acgtcgctaa gcgcccggta atatcactgc ggatttaagc
    13681 gagcgttttc cccaccacgt gaactttgcc gatatacggg tagaacatgg tgccgtcagg
    13741 ctgtacccag ttgccggtgt cgctggagct gcggtactga ccggctggcg tggtgagttc
    13801 cgggtgatcc cagacggtga cattaagaac gtcccccggc ccgacgcgat actggtaatt
    13861 cgcgatctca ctttccaggg gcatattggg gcgcgctaca ttcgggcgtg gacgtaattg
    13921 atcaatcagg cgcggggtca acggataaac attcaccatt ttgtcgagat cgaaatcagc
    13981 gtcctgctgt ttgatgacgt ctttacccat cgtcgacata ttgctgcccg taagtactgt
    14041 gcaaccgctt atcaaggtta ctgataccaa taatggcatc aatttcattt tggatttcat
    14101 cattgtttat ttatcacttt ggcagagtaa ttatcctgtg cactattaat agcaatgtcg
    14161 ccatgcacat ttaccttgca gttaattgaa taaaaattta actggcatca gtcctaaaaa
    14221 aattgatttc atccgcaggc tattgacaga acaattcaga ctggtctttc aggcatccag
    14281 acacgctacc gcccctggct ttttagctac caatacactg atttagctta atttttaaaa
    14341 ccctctcaac atacagtcgt tgatgagaaa gggttattgc ggaaattaac ttccgaatat
    14401 aaggtgacat tatggtaatt gaatattggc tctccaataa tgcaggagga agtgttacag
    14461 ctaacggaat agcaggcaag gtaacaattc ggcaattggc tatttttaag aattatatta
    14521 agtgtcattc aatatggttt ttaggagttt ctttaggttg acaatattta atatagtgtc
    14581 tccacatgcg atatttctta aataatgtct tattattacc acttttaatt cagggataat
    14641 gtgaggttat aaacctcaaa taatatgaga taaatagtgc tgcaacattg catttttgcc
    14701 ccgatttatc catgatcgaa ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac
    14761 tatggaatgg attgccgatc cgtctatctg ggccgggtta atcacgctga ttgtgatcga
    14821 actggtcctc ggcattgata acctggtctt tattgccatc ctcgccgaaa aactaccgcc
    14881 gaagcagcgc gaccgcgcac gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt
    14941 actgctggcg tcaatctcct ggctggtcac cctgacacaa ccgctattca gctttcgctc
    15001 gtttaccttt agcgcccgcg acttaatcat gctgtttggt ggtttcttcc tgttgttcaa
    15061 agccacgatg gagctgaacg aacggctgga agggaaagac agcaataacc ccacgcaacg
    15121 caaaggtgcg aagttctggg gggtggtgac acaaattgtg gtactggacg ccatcttctc
    15181 actcgactcg gtgattaccg ccgtcgggat ggtcgaccat ttactggtca tgatggccgc
    15241 cgtcgttatc gctattagcc tgatgttgat ggccagcaag ccgttaacgc aatttgttaa
    15301 cagtcacccg acgatcgtta ttctctgctt aagcttcctg ttgatgattg gctttagcct
    15361 ggtggcagaa ggtttcggct tcgtcattcc gaaaggctac ctgtacgctg ccattggttt
    15421 ctcggtgatg atcgaggcgc tcaatcagtt ggctatcttt aaccggcgac gttttctttc
    15481 cgctaaccag acgctgcgcc agcggactac cgaagcggta atgcgcctgc ttagcgggca
    15541 aaaagaagat gcggaactgg acgccgaaac cgcgtccatg ctggtggatc atggtaacca
    15601 gcagatcttt aatccgcagg aacggcggat gattgagcgg gtacttaatc ttaaccagcg
    15661 tactgtcagc agcattatga cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc
    15721 ggaagaagaa atccgccagt tgctggagcg aaatcagcat acgcggttgg tggttaccga
    15781 cggtgatgac gcagaagatt tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc
    15841 tctgcgcggc gaaccgctca acctgcgggt gttgattcgc cagccgctgg tgttcccgga
    15901 aaccttgacg ttgttacctg ccctggagca gttccgtaat gcccgcacgc actttgcttt
    15961 tgtggtggat gagtttggct cggtggaagg gattgtgaca ttaagtgacg tcactgaaac
    16021 cattgccggt aacttaccga acgaagtgga agagatcgac gcccgccatg atattcagaa
    16081 gaatgcggac ggttcctgga cggcgaatgg tcatatgccg ctggaagatc tggtgcaata
    16141 tgtgccgctg ccgctggatg aaaaacgtga atatcacacc attgccgggc tgttgatgga
    16201 atatttacag cgtattccaa aacctggcga agaagttcag gtgggggatt atttgcttaa
    16261 aacgttgcag gtggaaagcc atcgcgtgca gaaggtgcag attattccgc tgcataagga
    16321 tggcgagttg gagtacgagg tgtgatggta tcggtcattt tacccaatag ccgaaaggaa
    16381 taagatgagg gaaccgcgcc agcggcgatt taccgccggg agcccgggtt gccagggtgg
    16441 tggagattga gccaccctgg cacgttcacc ggcttgagcg ttccatagta gcgaggaaca
    16501 tcaagtgaac ggaacagcct tcgatcttac atattcagaa tataatgatg ccgccacgaa
    16561 cgcctgatgc gacgctttac gcgtcttatc aggcctacag gactggtcac ctggtaacct
    16621 acatcttctc cagcaacttc ttcacatcct tgccattgcg ggaatcttta ttccgctcgg
    16681 cccagtcatt caggcgacgt ttcgcttcgt cctgtagatg tttgcgcagt aactgatcca
    16741 cttgcagact gtaattgagt tgctgccagt tgccataaac ccgcagcggt actggcgttt
    16801 ctttcagaaa atcaatcagt ttactttccc cgttccagcc acccacgacc cgaatatcaa
    16861 actgggtgtc gcaggtttga tctgccagat tcaacatgcc ttcccctgtc agcgccagca
    16921 caggcgattg accttgcatg tcgtttaacg tcacgacgcc atccttcaac gtcaaatcgg
    16981 tggtaaagcg gtcaagccgc gtcacgttat caaagttttc agcggccttc acatcaccgc
    17041 cattacgctc taccgcttgc tgaatcatct gctggaagtt catcccttcc atgcgcgtgt
    17101 cggtcatttc gacatgtgcc tgcccttgcc agttgtggcg gaatgcgtcg gcatctatgt
    17161 cagcaccgga gaaatcacca gccagtgaca tttttccggt caacgaaatc ggatagttaa
    17221 acgccttcag aatggtacca atctcaacgt tttccagccg tggctggaag tttatccgcg
    17281 gatttattga tgtcgcgtcc agcgtgcccg gcagtgaaac ctgtccaccg ttaagcttgc
    17341 cctgcagttg agtaatttcc agcaaacccg acttgttggt catttgcgtg gcaacatctg
    17401 taaaattcat tccgcgccag cgcacgttac tggcctgcaa caaaatatca gccgtaaagc
    17461 cttgcagtcc ctgataggcc ggttcatcaa tacgcgaaga aataaccggg cgcggcaacg
    17521 ttgattggct ctgcccctgc tgcgcggcac cgttttcacc attcgcggtt tcattaagcg
    17581 ggatgaggtt gtcaagattc agttgcggga attgcagtct cagctgccat tcaggtttct
    17641 ctgtcagcgt gacctgtgct tgcccgctca gcgtactgtc attggcggtc aagctgattt
    17701 ggttaaatga aaggcgttta tgtgactcct gccactgggc ctggaaactc ccctgcccct
    17761 gaataccttg ttttggtaaa tcggcaccct gcaactgcca gttaatttgt tcaatagcag
    17821 ccgttaaatc atgcggataa tcagaagcat ctaccgtacc gttaagggat atcgtgagat
    17881 cgcgctgatc gcgattaacc cgcccggaga actcaaatga accacgatgt tggggatctt
    17941 gttccatttg caggcgaata ttgcggattg tcacctgctc gtcatcttca tgctggaaca
    18001 ccagcacgct gtccgccacc ttaagactgg ttatatcaaa cgaccatccg cgatcgtctg
    18061 acagatccgg caaggtattg tcgcgcggtg caaccggagc gtcttcactg cgcaccgctt
    18121 ccgtctgcgg cgtcagttgg atcactgccc cttttagcat cacctgctta acgctcagtt
    18181 gatgactcag tagtggtaaa agcgccacgt ccagacgcat gttgtcggcg cgaaccagtg
    18241 gctggctcgc gccctgggcg gtgagagaca ttcgcccgga gaggatacta agctgcggcc
    18301 agacgtgcca acgcagtggc ccgtcgagct gcaattgata accgctacgt gcagcaactt
    18361 gcttgaccat atagtcgcgg aaatcattcg gattcaccag caacactaac gcagataacc
    18421 cggccaccag cacgaccagg agtatcatca gcgtcgtcag aaatcgtctc atggtatcct
    18481 caatgggcct gaattagtct ttatcgattc ggctggctac cgcgccctgc tggttgcgat
    18541 atttcgcatc ttcacggcgg ttgtaaggtc gcgccgccgg gccggaaagc ggctcaaagc
    18601 tcagcgcacc aattaacatg cccggacgca gcgccagcgg cagcttaccg gagttgtaga
    18661 actccagcac aatacagcca gaccagcccg gatcgatacg gtgcgcggtg acgtgcacca
    18721 tcagccccag acgcgccagt gaggaacgac cgtccagcca tcccaccaga tcggctggca
    18781 gcgtcaccga ctccagcgtc accgccagcg ccagctctcc tgggtgaaga taaaatgcct
    18841 cgctttcatc gagtacgatc tcatcgctca tcacgcggtc aagcgcggcg ctcacttcat
    18901 ctttgggacc gctcagatcg ataaacgctg ccgtgtgacc acggaaggta cgaaatttat
    18961 tgcccaggcg tacatccacc gtcgcgccgt taatacgctc cactggcgga cgtgggttga
    19021 tcgacaaacg gccttcatca agccaggctt caatatctcg gtcacacaga cgcatggcat
    19081 tttctccttt cgcgcatcac tcccttaacg ccaattacgt caagggcata ccaggttatc
    19141 actgaacggt acacaattta tcaagcttat tcaaagaact gacttatttt cgctttcaat
    19201 atatcgatcg caatgcggtt tttcccgccg cgcggcacga taatgtccgc atattgttta
    19261 gaaggctcaa tgaattgcag gaacatcgga cgcacggttt tttgatattg cgccatcacg
    19321 gaatccattg aacgcccacg ctcgttaacg tcacgcttga tgcggcgcat caggcagata
    19381 tccagcgggg tatcaacgaa aatggagaag ttaagttcgt cacgcaaacg cgcatccgtc
    19441 agcaacaaaa tgccttcgag aatgatgact ttcttcggct caaccgtcac cgtttctttc
    19501 atacgcgtat gttcaacata gctgtaaacc ggcaggtcaa ttgccgagcc gcgtttcaac
    19561 gcttgtaaat gctcaagcaa caggctgtga tccatcgcgc taggatggtc gtagttggtc
    19621 ttaatgcgtt cttccatcga cagatggctt tgatctttgt aatagcagtc ttcgggaatt
    19681 acgccgatgt gttcatcacc gacttgctca cgtaattcac gataaagggt actggcaata
    19741 agactcttgc cggaagccga tgcgccagcg ataccgataa tgacgcactg atgagactgt
    19801 tcagtcatat atttagcgac ctgattaacc tggatgttag gaagggggcg acgaagcgcc
    19861 aaacgcggca attataggga tttcatccgc ctgataccag tcgaatagcg ttgccgcgcg
    19921 ctcagagtta attgttgacg aagaattccc ggtggcaaat tacgttgatc agttttatac
    19981 aaggtaaaaa atgttatacg cagttgcgca aattatccgc ctttacgtca ctttatgagc
    20041 aattcgcata taaaatgtaa aacttttgta ctagcataaa cacagaaaca aatactggcg
    20101 acctggtctt gcggataaag cggtaatgag caaacaatca cagcatgtat taattgccct
    20161 gccccacccg ctgcttcacc tggtcagttt aggtttagtc tcgtttatct ttaccctttt
    20221 ctcgcttgag ctttcgcagt ttggcaccca actcgcccca ctgtggttcc cgacgtccat
    20281 catgatggtg gcgttttatc gccatgccgg gcgcatgtgg ccgggaattg cgctgagctg
    20341 ttcgctggga aatatcgccg catccatcct gcttttttcc accagctcgc tgaacatgac
    20401 ctggacgacc atcaatattg ttgaagccgt ggtcggggca gtgctgctac gtaaattgct
    20461 gccgtggtat aaccccttgc aaaatctggc tgactggctg cgtctggcac tcggcagcgc
    20521 cattgttccg cctctgttgg ggggtgttct ggttgtcctg ctgacgcccg gagacgatcc
    20581 tctcagggca tttttgatat gggtactgtc agaatccatc ggcgctctgg cactggtgcc
    20641 gctgggattg ttatttaagc cacactatct gctgcgccat cgcaacccac ggttactttt
    20701 tgagtcactg ctcacgttag ccatcacgct gacgttaagc tggctttcta tgttgtacct
    20761 gccgtggcct tttactttca ttattgtgct gttgatgtgg agcgccgtgc gcctgccacg
    20821 aatggaagcc tttttgatct tccttaccac ggtgatgatg gtgtcgctga tgatggccgc
    20881 ggatccctcc ctgcttgcta cgccgcgtac atacctgatg agccatatgc cgtggctacc
    20941 gtttttgctg atcctgctgc ccgccaatat catgacgatg gtgatgtatg cctttcgtgc
    21001 ggaacgcaaa cacatttccg aaagcgaacc ccgttttcgg aacgccatgg aatattccgc
    21061 tatcggtatg gcgttagtgg gcaccgaggg acaatggctg caaaccaaca aagcgctctg
    21121 ccagtttctc gggtacagtc aggaagagct gcgcggactc acctttcagc aactgacctg
    21181 gccggaggat ctcaataaag atctccaaca ggttgaaaag ctgataagcg gtgaaataaa
    21241 cacctattca atggaaaaac gctactacaa ccgcaatggc gatgttgtct gggcgttgct
    21301 tgccgtctca ctggtgcgcc acacggatgg cacgccgctc tattttatcg ctcagattga
    21361 agacattaac gagctaaaac gcaccgaaca ggtcaatcag caactgatgg agcgcatcac
    21421 gctggctaac gaagcgggcg ggattggcat ctgggagtgg gagttgaagc cgaatatttt
    21481 tagctgggat aagcggatgt tcgagctgta tgaaattcct ccgcatatca aaccgaactg
    21541 gcaggtgtgg tacgagtgtg tattgccgga agatcgccaa cacgccgaaa aagtgattcg
    21601 tgattcgttg caatcacgct cgccctttaa actggaattt cgcattaccg taaaagacgg
    21661 tattcgccat atccgcgccc tcgccaaccg ggtactgaat aaagaaggcg aagtcgaacg
    21721 tctcctcggc atcaatatgg atatgaccga agtgaaacag cttaacgagg cattgtttca
    21781 ggaaaaagag cgcctgcaca ttacgcttga ttccatcggt gaagccgtgg tctgtattga
    21841 tatggcgatg aaaattacct ttatgaatcc agtggcggag aagatgagcg gctggacgca
    21901 ggaagaagcg ttaggtgttc cgctcctgac ggtgttgcat attacttttg gcgacaacgg
    21961 accattaatg gagaacattt acagtgccga cacctcacgt tccgcgatcg agcaagatgt
    22021 ggtgttgcac tgtcggagcg gcggcagtta cgacgtgcat tacagtatta cgccgttaag
    22081 tactctggac ggcagcaata ttggttcggt tctggtgatt caggacgtta ccgaatcacg
    22141 caaaatgctg cgccagctga gctacagcgc ctcccatgat gcactgacgc atctcgccaa
    22201 ccgcgccagt tttgagaaac aactgcgtat cctgctgcaa acggtaaaca gtacacatca
    22261 gcgacatgcc ctggtgttta tcgatcttga tcgctttaaa gcggtgaatg acagcgccgg
    22321 gcatgcggcg ggtgacgctt tactgcgcga actggcgtcg ttgatgctga gtatgctgcg
    22381 ctccagcgac gtgctggcgc gactcggtgg tgatgaattt ggtctactgt tgccagactg
    22441 taatatcgaa agtgcgcgtt ttatcgctac acgcattatc agtgccgtga atgattatca
    22501 ctttatatgg gaaggccgtg tgcatcgggt aggtgccagt gccgggatta ccttgattga
    22561 tgacaacaat catcaggcgg cagaagtgat gtcgcaggct gatatcgcct gttatgcctc
    22621 caaaaatggt ggacggggcc gggtgacggt ttacgaacca cagcaagctg ccgcacatag
    22681 cgaacgggcg gcgatgtcgc ttgatgaaca gtggcggatg attaaagaga atcagttgat
    22741 gatgatggcc cacggtgtcg cttcgccgcg gatcccggaa gcgcgtaatt tgtggctgat
    22801 ttcacttaag ctctggagtt gcgaaggcga gattattgat gaacaaacat ttcgtcgtag
    22861 cttcagcgat ccggcgctta gccatgctct tgaccgccgg gtattccacg aatttttcca
    22921 gcaggccgca aaagcggttg ccagtaaagg cataagcatc gccctccccc tttccgttgc
    22981 cggtttgagt agcgccacgc tggtgaatga tctgcttgag cagctggaaa atagccctct
    23041 accaccacgg ttattacatc tgattattcc ggctgaagcg attttagatc acgcagaaag
    23101 cgtgcaaaaa ctgcggctgg cgggatgtcg gatagtgctc agccaggtgg gccgcgatct
    23161 gcaaatcttc aactcgctga aagcgaatat ggcagattac ctgctacttg atggtgagtt
    23221 atgcgccaac gtgcagggta atttgatgga tgagatgctg attacgatta ttcaggggca
    23281 cgctcagcga ctcgggatga aaaccatcgc cgggccagtc gttttaccct tagtgatgga
    23341 tacgctttct ggcatcggcg tcgatctgat ttatggtgag gtgattgccg atgcccaacc
    23401 gctggatttg ctggtgaata gtagttattt cgcgattaac tgaggcgtgc ttccccatcg
    23461 cctgatgcga cgctaacgcg tcttatcatg cctacaaatc gctcattccc caggccggat
    23521 aaggcgctcg caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc cagccttccg
    23581 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
    23641 ggatttgcgc cggtgtcatt cccggaaatc gctgtttaat cagataatca tccggcagga
    23701 aaacatcttt cgcctgccag ccacgcaaag caaaataatt cgccgtccag ctcccgatac
    23761 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggtatt gtcattggta
    23821 aggtgccctc cagcgccgca tttgccagat gaatcagcgc ctctgcccgt ttcaacggca
    23881 tacctaacgc ttttaatgcc tgcgggtctg ctgctgccag ccgctgaggc gtggggaagc
    23941 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtgccact ctggcggtca
    24001 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcacg ccctgctcaa
    24061 aagcatcaac actgctgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg
    24121 caccgttaac aatctgcggg ttacattgca gatcaagcag gcggctcatt ttcgccagac
    24181 actcagcggc aacgggttct aaacctacac ttaaatttat gtgcagagta tggcgggcta
    24241 tatccggaac agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat
    24301 aactgtccgc gaccgtttcc acaccgctca ccgcacgggc ggcgagaaat cccaacatcc
    24361 acgaccagtc atacggcgac tgccagttca gggtatacat cgcatctcct tattcatccg
    24421 ctttcagcat aaacgttatt cagacgctac gctttgcttt catattccgg ttgtcgcgac
    24481 ggcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta
    24541 ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc
    24601 aattactgaa aatggaaaac gacagcacgc tgctgccttc aatgctttgt gcgccaacgc
    24661 gtgaagcggt aagcgaatgg ttgtaccgcc atcatgatgt tccggcagac gacgatgaaa
    24721 cgcaggcgct gttgcgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg
    24781 cgaaaagcgt gcagttcgga ctttcctcac tggcacagta cattgatgat ccggaagaag
    24841 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    24901 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg
    24961 cacagctgcc agaaacgatt actcaggcgg tgatcggtcg ccctatcaac ttccaggggc
    25021 ttggcggcga tgaagcaaac acccaggcgc aggggattct ggaacgcgcg gcgaagcgtg
    25081 ccgggtttaa ggatgtggta ttccagtacg agccggtagc ggcagggctg gattacgaag
    25141 cgactttgca ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt acgactgact
    25201 gttcattgct gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc
    25261 tgggtcacag tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa
    25321 acctgatgcc actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc
    25381 tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta
    25441 gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg
    25501 ccctgctaca gaaagtctgg cgtcagcgtt taagttatcg cctggtgcgt agcgcagaag
    25561 agagcaaaat tgcgctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagcg
    25621 atgaactggc tacgctgatt agccagcaag ggctggaaag cgccctcagc cagccactga
    25681 cgcggattct ggaacaggtg caactggccc tggataacgc ccaggaaaaa ccggacgtta
    25741 tctacctgac cggcggaagc gcccgttctc cgctgattaa aaaagcgctg gcagaacagt
    25801 tgccgggcat tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac
    25861 gctgggcgga agtggtgttt cgttaattga aaaatggctc agcagcggct taaatctgcc
    25921 tgcgcctgag ccagtttttc tgccgcctga cgtaacggtt ccatcaacgg catggtccgt
    25981 gctggagcat gaaggcggaa tcgctgcaac tctgtcgcgc cgacagtcaa cgccgattcc
    26041 agccgatgtc gtttggcggc cactttcgcc attaccgctt gtctgtctgc aggcgttacc
    26101 gcttcgttcg gcctgaacac aaaacggcgt tcacagctcg ctttccagtc gatgaccgcc
    26161 tgggtcagat gatcaccaaa ccctttaact tgcttaaccc cacgacgggt aacatccgct
    26221 gctgtttcaa taccaaagga ccgtaacgcc gctttacgcg cagggccaac accgggaata
    26281 gaggcaacat caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcggtg
    26341 tcgtgaagtg ctgccagagc acgtttttct tcctccggta acccgagcat ttcgtccttc
    26401 attttttcga gcatcgtccg tttggcgata aaaccttcca gcccgcccaa ctgttggatc
    26461 tggctgacta aatggtcata gtccattttc gcgcgattaa atgcctcacg gcgttgctgg
    26521 atttctgctt tataagcttt gcttgtcaga ctgccaataa tccagatagc cgccagcaca
    26581 ggaaccaaaa taatataacg cggttctgcc tgaaggccgc aaagcaacag cgataacgct
    26641 gacagtgcga tctcaagcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc
    26701 cggccagtcg gttggaaatg atcgggtaat ggcaattgca atgctggcgg tgctactgac
    26761 gccatcacca tcgcccagac tttcgccagc acaaaatcac cgctggtggt aatgacctct
    26821 tcgccgagat caataaaata gataacgcct tgattatcca gcgcacacca cgggcagtca
    26881 gtcaaatgac cggggtaaac atgcattgcc gaaacggtac atttctttaa ctgttggcgt
    26941 agagaatcca gtgctgatac ccacgctttt gccgtcggac gcccggttgc cacgccactt
    27001 tccgtgaatg cctgctgaaa catggcttca acatcactcg gtaacatcga tagcggaatc
    27061 gatcgtggcg gcggttttaa accacgccgc tgattatctg actcgtaggc ataacggaaa
    27121 tgagcaatat ccgtctccag cgcattcccc gcatcagaga taagcggaac accggaataa
    27181 ggatgccgcc caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga
    27241 ttttctgtgc gttcaaagcc gacaaatgac gacatggttt gtagctctgg cggcgtaaaa
    27301 tgcgacacgc cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtcg
    27361 ctatcaatca acaccacttt gctgtcgcga cctaccataa aactgttctg gtttacgtca
    27421 cccacgacgt gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca
    27481 tagagtagaa aatcccacgc acaatgaggg taactctggc gacgatgtgc cgggctatag
    27541 atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg
    27601 cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa ctgcgcgtca
    27661 gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa
    27721 atcttggcga cgctatcggc aaactcctcg atatcataaa ccgcaccttc accgcctttg
    27781 cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg
    27841 gtaaccacag cgccagtgcc aacgttttat catcatcggt acgctcgttt accgccgggc
    27901 tcgacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgccg
    27961 ccagtccatt gaaaaacggg gtaaagaaag gcacatgcgg agaattatcc agcatattta
    28021 acgccagtcg ctgaatacca tccgtaaatg ctgcaacttt atgcgcacgc tcagtgctgg
    28081 tgaaagtttc cagtctggaa acagcatctt catcgctaat gaaatgcgtc atattggcat
    28141 attcacccac catcggcgtg agcggcagtt ccagaccacg accaaaatcc accactacac
    28201 cgccgtcgcc aatttgcata atcaatgtgc cgttagccga tgaaatcaat cccagaaacg
    28261 tgcaggcaaa atcgcgaact gctaatgctt tggcttctgc ctcggcaaaa agtcgttgac
    28321 gaatggtcag taccatattt gtggcgagga catcattaag ccccaattca ccgtcctgta
    28381 ctttttgcac cataaacgcc atcgcttcat tgacggcgag cttcgcgcct tctcctccct
    28441 gcgaaacgct acctgcgcca tcagcaaaga acatcaccag taatggctgc tggtcattta
    28501 gccaggcaac ctgcatctgg caggcatcct gacaaggtaa atcagcgcta atgtgcgacg
    28561 ttccaacagc ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc
    28621 ttccagcaca acttccgttc ccggcgtgga gcaggaaacc gaacgcagtg aactggataa
    28681 ccagctaaac agttcgcgga attgtaatcc ctgcagagac aaaggctgac ggacgctgat
    28741 ttgggccagc gtcttcatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt
    28801 atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggagcgcc
    28861 atcggtgatc aggaaaatcc acgggcgata atacgaaata ccatttgcac gatactcacg
    28921 tttccgctcc tctaccatat ccagggcttt ggtaatggcg gcacccattg gtgtatcgcc
    28981 ctgggcaaac aggatgggcg ggaaaaaatt agcggcgctg gtaaagggtt gttccacatg
    29041 caccgggccg aacgtcacaa taccaagttc cactcttttt aaggccagcg ggtcggcaag
    29101 cagctcatcg cgaaaggtaa ccaatccggc gttaagttca ttaatgggtc taccgttcat
    29161 cgaaccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa
    29221 atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattattg
    29281 aggtccggta tcataccgat tgcaataatg aaataagagc aaacgaagaa actttcagaa
    29341 ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat
    29401 tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggagag gtttccccct
    29461 ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga
    29521 cgtgcttcat cataaccctt tccttattta aaagccctct tctccgggag agggttcccc
    29581 gttttagcgt cccgctgaaa tcgtcggctt acctcctttc gccctgaatg cagtctatcg
    29641 ctgacttgcg gataaaatag tttcaagtgt gattattgcg taacgactcg caaagaatat
    29701 ttgctggcag gatcgcagac tgcaaagcct gcagattgac aatcttatcg tgaaggcata
    29761 ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag taagcgggga
    29821 agcttatgac taagagcacc acgatgatga gtagcttcat catgaccctt tccttattta
    29881 aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc ttcggcttac
    29941 ctcctttcgt cctgtatgca gtctatcgct gacttgcgga taaaatagtt tcaagtgtga
    30001 ttattgcgta acgcctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc
    30061 ggattgacaa tctgaccgtg aaggcatact ttcaggagtg agggttgagc ggggtttccc
    30121 ccgccctggt agtcttagta agccgggaag cttatgacta agagcaccac gatgatgatc
    30181 cacttcatca tgaccctttc cttatttatg gccccttcct cgggaggggc tttcccgttt
    30241 cagcgtcccg ctgaaatcgt cggcttacct cctttcgcca tgcaagcagt ctatcgctaa
    30301 cgcgtagata aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgcc
    30361 attcagacga ttcccgatag tgtttcataa ttcctccatt tttctccctt attggctggc
    30421 tacactagta tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag
    30481 ttataaatcc cgttgggtaa tcgtaatcgt ggtggttatc gccgccatcg ccgcattctg
    30541 gttctggcaa ggccgcaatg actcccagag tgcagcccca ggggcgacga aacaagcgca
    30601 gcaatcgcca gcgggtggtc gccgtggtat gcgttccggc ccattagccc cggttcaggc
    30661 ggcgaccgcc gtagaacagg cagttccgcg ttacctcacc gggcttggca ccattaccgc
    30721 tgctaacacc gttacggtgc gcagccgcgt agatggtcag ttgatggcgt tacatttcca
    30781 ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca gccagttcaa
    30841 agttgcatta gcacaagccc agggccaact ggcaaaagat aaagccacgc ttgccaacgc
    30901 ccgccgtgac ctggcgcgtt atcaacaact ggtaaaaacc aatctcgtat ctcgtcagga
    30961 actggatgcc caacaagcgc tggtcagtga aaccgaaggc accattaagg ctgatgaagc
    31021 aagcgtcgcc agcgcacagc tgcaactcga ctggagccgc atcaccgcac cagtcgatgg
    31081 tcgcgttggt ctcaagcagg ttgatgttgg taaccaaatc tccagtggtg ataccaccgg
    31141 aattgtggtg atcacccaga cgcatcctat cgatttgctc tttaccctgc cggaaagcga
    31201 tatcgctacc gttgtgcagg cgcaaaaagc cggaaaaccg ctggtggtag aagcctggga
    31261 tcgcaccaac tcgaagaaat taagtgaagg cacgctgtta agtctcgata accaaatcga
    31321 tgccactacc ggtacgatta aagtgaaagc acgctttaat aatcaggatg atgcgctgtt
    31381 tcccaatcag tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg ccgtagtgat
    31441 cccaacagcc gccctgcaaa tgggcaatga aggccatttt gtctgggtgc tgaatagcga
    31501 aaacaaggtc agcaaacatc tggtgacgcc gggcattcag gacagtcaga aagtggtgat
    31561 ccgcgcaggt atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc gcctgaccga
    31621 aggggcgaaa gtggaagtgg tggaagccca gagcgccacc actccggaag agaaagccac
    31681 cagccgcgaa tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc cccgagcagc
    31741 acaggcggcc cgtcgcgcct gtttattatg cgtcctgtgg ccaccacgct gctgatggtg
    31801 gcgatcttac tcgccgggat tatcggttat cgcgccctgc ccgtttcggc gctgccggaa
    31861 gtggactatc cgaccattca ggtggtcacg ctctacccag gtgcgagccc ggatgtcatg
    31921 acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg tctgaaacag
    31981 atgtcgtcac aaagttccgg cggtgcgtca gttatcactt tgcagttcca gctaacatta
    32041 ccgctggatg tcgccgagca ggaagtacag gccgcgatta acgctgcgac caacttgttg
    32101 ccgagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga tccgccgatc
    32161 atgacgctcg ccgtcacctc aacagccatg ccgatgacgc aagtggaaga tatggtggaa
    32221 acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtcg gcctggtgac gctttccggc
    32281 ggccagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc cctcggcctg
    32341 accagcgaaa ccgtgcgcac cgccattacc ggcgctaacg ttaactcggc aaaaggtagc
    32401 ctcgacggcc cttcccgcgc ggttacgctt tccgcgaatg accaaatgca atctgcggaa
    32461 gaatatcgcc agttgattat cgcctaccag aacggcgcgc cgattcgtct gggcgatgtc
    32521 gcaactgtag agcaaggtgc agaaaacagc tggctcggcg catgggcgaa caaagaacag
    32581 gccattgtga tgaatgttca gcgccagccc ggtgctaaca ttatctccac cgccgacagc
    32641 atccggcaga tgctgccaca gctcaccgag agtttgccga aatcggtgaa ggtgacggta
    32701 ctttctgacc gtaccaccaa tatccgcgca tctgtcgatg atactcagtt tgaattgatg
    32761 atggctatcg cgctggtagt catgattatc tacctgtttt tgcgcaatat tccggcgacc
    32821 atcattcccg gtgttgccgt gccgctgtcg ttaatcggca cttttgcggt tatggtgttt
    32881 ctcgattttt caatcaataa cctgacactg atggcgttaa ctatcgccac cggattcgtg
    32941 gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa
    33001 ccgttggcgg cggcgctcaa gggcgcaggt gaaatcggct ttaccattat ctcgctgacc
    33061 ttctcactga ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt cgggcgactg
    33121 ttccgcgaat ttgctattac cctggcggta gcgattttga tctcagcggt ggtgtcgctg
    33181 accctgacac cgatgatgtg cgcgcggatg ctcagccagg agtcgttgcg taaacagaac
    33241 cgcttctccc gtgcctcgga aaaaatgttc gacaggataa tcgccgccta tggtcgtgga
    33301 ctggcgaagg tgctgaatca tccgtggctg accttaagcg tggcgctcag cacgctgctg
    33361 cttagcgttc tcttatgggt gttcattccg aaaggtttct tcccggtaca ggacaacggc
    33421 attattcagg gcactttgca ggcaccgcaa tccagctcct ttgccaatat ggcccagcga
    33481 caacgccagg tcgcggacgt gattttgcag gatccggcag tgcaaagcct gacctcattt
    33541 gttggcgttg atggcactaa cccgtcgctg aatagtgcac gtttacaaat caacctcaaa
    33601 ccgctggatg aacgtgatga ccgagtgcaa aaagtcattg cccgtctgca aacggcggtg
    33661 gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggatctgac tattgatact
    33721 caggtcagcc gcacccagta ccagtttacc ttgcaggcca cctcactgga tgcgctcagt
    33781 acctgggtgc cacagttgat ggaaaaactc cagcaactgc cacagctttc tgatgtctcc
    33841 agcgactggc aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc
    33901 cgtctgggga tcagcatggc ggatgtcgat aacgccctgt acaacgcgtt tggtcagcga
    33961 ctgatttcca ctatttatac tcaggctaac cagtatcgcg tagtgctgga gcacaacacc
    34021 gaaattaccc cgggcctcgc ggcgctggat accattcgcc tgaccagtag cgacggcggc
    34081 gtggtgccgt taagctcaat tgccaaagtt gaacagcgtt ttgcgccgct ttccatcaac
    34141 catctggatc agttcccggt aacgaccatc tcctttaacg tgccggataa ctattcgctg
    34201 ggcgatgcgg tgcaggcgat tatggacacc gaaaagacgc tgaatctgcc ggtggatatc
    34261 accacgcaat ttcagggcag caccctcgcc ttccagtcgg cgctgggcag cactgtctgg
    34321 ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta cgagagcttt
    34381 attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc gctgctggca
    34441 ttaatgattg ccggtagcga actggatgtg attgcgatta tcggcattat tttgctgatc
    34501 ggtattgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc tgagcgcgag
    34561 caaggcatgt cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt tcgtccgatc
    34621 ctgatgacca ccctggcggc tctgcttggc gcgctgccgc tgatgttgag taccggggtc
    34681 ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat tgtcagccag
    34741 gtgctgacgc tgttcaccac gccggtgatt tatctgctgt tcgaccgcct ggcattgtgg
    34801 accaaaagtc gctttgctcg ccatgaagag gaggcgtaag tgaagttttt tgccctcttc
    34861 atttaccgcc cggtggcgac gattttactg tcggtcgcca ttaccctgtg cggcatactg
    34921 ggcttccgta tgctgccggt cgccccgctg ccgcaggtcg attttccggt gattatggtc
    34981 agcgcctcgc tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc cacgccgctg
    35041 gagcgctcac ttgggcgcat tgccggagtc agtgaaatga cctccagcag ttcgctcggc
    35101 agcacgcgta ttattttgca gtttgatttt gaccgggata tcaacggcgc ggcgcgcgat
    35161 gtgcaggcgg cgatcaacgc tgcacaaagt ttgctgccca gcgggatgcc cagccgtccg
    35221 acctatcgca aagctaaccc gtcggatgcg ccaattatga tcctcacgct gacatccgat
    35281 acttattcgc agggtgaact gtacgatttt gcctcgacgc agctggctcc gacgatttcg
    35341 caaatcgacg gtgtcggtga tgtcgatgtt ggcggcagct cgctgcccgc cgtacgcgtc
    35401 gggctgaatc cgcaggcgct gtttaatcag ggcgtctcgc tggacgacgt acgcaccgcc
    35461 atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg aagatggcac tcaccgctgg
    35521 cagatccaga ccaatgatga gctaaaaacc gccgccgaat atcagccgtt gattattcat
    35581 tacaacaacg gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga ctcggtgcag
    35641 gatgtgcgca acgccgggat gaccaacgcc aaaccggcta ttttgctgat gatccgcaag
    35701 ctgccggaag ccaatattat ccagacggta gacagcatcc gggcaaaatt accggagttg
    35761 caggaaacca ttccggcggc gattgatctg caaattgctc aggatcgctc ccccaccatt
    35821 cgcgcctcgc tggaagaagt cgagcaaacg ctgattatct cggtggcgct ggtgattctg
    35881 gtggtgtttt tattcctgcg ctcgggtcgc gccactatta ttcccgccgt tgcggtgccg
    35941 gtttcgctga ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct caataacctt
    36001 tcgttaatgg cgctcaccat cgctactggt ttcgtggtgg atgacgccat cgtggtgctg
    36061 gaaaacattg cccgtcatct ggaagcgggg atgaaaccgc tacaggccgc gctacaaggt
    36121 actcgcgaag tcggctttac ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg
    36181 ccgctgctgt tgatgggcgg attgccgggc cgactgttac gcgaatttgc cgtgacgctt
    36241 tctgtcgcca ttgttatatc gttgctggtt tctctgacat taacgccaat gatgtgtggc
    36301 tggatgctga aagccagcaa gccgcgtgag caaaagcgac tgcgtggttt tggccgcatg
    36361 ttggtagccc tgcaacaagg ctacggcaag tcgctgaaat gggtgctcaa tcatacccgt
    36421 ctggtgggtg tggtgctgct tggcaccatt gcgctgaata tctggctgta tatctcgatc
    36481 ccgaaaacct tcttcccgga gcaggacacc ggcgtgctga tgggcgggat acaggcggat
    36541 cagagcattt cgtttcaggc gatgcgcggc aaattgcagg atttcatgaa aattatccgt
    36601 gacgatccgg cagtggataa tgtcaccggc tttaccggcg gttcacgagt taacagtggg
    36661 atgatgttta tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca gcaaattatc
    36721 gaccgtctgc gcgtaaaact ggcgaaagaa ccgggggcga atctgttcct gatggcggta
    36781 caggatattc gcgttggtgg gcgtcagtcg aacgccagct accagtacac gttgttatcc
    36841 gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg
    36901 ccggaactgg cggacgtgaa ctccgatcag caggataacg gcgcggagat gaatctggtt
    36961 tacgaccgtg acaccatggc acggctggga atcgacgtac aagccgccaa cagtctgtta
    37021 aataacgcct tcggtcagcg gcaaatctcg accatttacc agccgatgaa ccagtataaa
    37081 gtggtgatgg aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc
    37141 gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg
    37201 aatgccccac tatcggtgaa tcatcaggga ttatcggcgg cctcgaccat ttcgttcaac
    37261 ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag
    37321 cttggtgtgc cttcgacggt gcgcggcagt tttgccggaa cagcgcaggt gttccaggag
    37381 acgatgaact cacaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg
    37441 ggtatccttt acgagagtta tgtgcacccg ctgacgattc tctccaccct gccctcggcg
    37501 ggtgttggag cgctgctggc gctggagctg ttcaatgccc cgttcagcct aatcgccctg
    37561 atagggatca tgctattaat cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt
    37621 gcgcttgaag cccaacggca cggtaacctg acgccgcagg aagctatttt ccaggcctgt
    37681 ctgctgcgtt ttcgcccgat tatgatgact accctggcgg cgctgtttgg cgcgctgccg
    37741 ctggtgttgt cgggcggcga cggctcggag ctgcggcaac ccctggggat caccattgtc
    37801 ggcggactgg taatgagcca gctccttacg ctgtatacca cgccggtggt gtatctcttt
    37861 ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg
    37921 acagatcttc ccgacagcac ccgttggcaa ttgtggattg tggctttcgg cttctttatg
    37981 cagtcgctgg acaccaccat cgtaaacacc gcccttccct caatggcgca aagcctcggg
    38041 gaaagtccgt tgcatatgca catggtcatt gtctcttatg tgctgaccgt ggcggtgatg
    38101 ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc
    38161 atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg
    38221 ttgctggcac gcgcgttaca gggcgttggc ggcgcgatga tggtgccggt cggcagattg
    38281 acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta
    38341 cccggtcagg tcggtccgct gctcggtccg gcgctcggcg gtctgctggt ggagtacgcg
    38401 tcgtggcact ggatcttttt gatcaacatt ccggtgggga ttatcggtgc gatcgccaca
    38461 ttgatgttaa tgccgaacta caccatgcag acgcggcgct ttgatctttc cggcttttta
    38521 ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg acggcagtaa aggtacaggt
    38581 ttatcgccgc tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat
    38641 ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt
    38701 accttttcgc tgggcctggc ggggagcttt gccggacgta ttggcagtgg catgttgccc
    38761 tttatgacac cggttttcct gcaaattggc ctcggtttct cgccgtttca tgccggactg
    38821 atgatgatcc cgatggtgct tggcagcatg ggaatgaagc gaattgtggt tcaggtggtg
    38881 aatcgctttg gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc gctggtcacc
    38941 ctgttgttta tgactaccgc tctgttgggc tggtactacg ttttgccgtt cgtcctgttt
    39001 ttacaaggaa tggtcaactc aacgcgtttc tcctccatga acaccctgac gctgaaagat
    39061 ctcccggaca atctggcgag cagcggaaac agcctgctgt cgatgagtat cggcgtcacc
    39121 atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga cagcggcacc
    39181 acacaaaccg tctttatgta cacctggctt agcatggcgt tgatcatcgc ccttccggcg
    39241 ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa
    39301 aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt ctggcgattt
    39361 tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc
    39421 gtggctttat tgattacatc aagcatggta atgaacaacg attacaactg ttaagtgatg
    39481 cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct
    39541 ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg
    39601 gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac aacaaagtgc
    39661 tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg
    39721 gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc aatactgata
    39781 tcaatttcga taaacaacag cggcaaacca gctggctgat cgtcgccctg gcaacgttac
    39841 tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct ggcaccggta aaacgacttg
    39901 tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg
    39961 aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcacg ctggagaaaa
    40021 accagcaaat gcggcgcgat tttatggccg atatttcaca cgaactgcgt acgccattag
    40081 cggtgctgcg cggtgaactg gaagccattc aagatggcgt gcgcaaattc acgccggaga
    40141 cggtggcgtc tttacaggcg gaagtcggta cactgactaa actggttgac gatcttcatc
    40201 agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta gatttgatcc
    40261 cactgctgga agtggcgggc ggcgcatttc gcgaacgatt tgccagccgt ggcctgaaac
    40321 tgcagttttc cctgccagac agtattaccg tatttggcga tcgcgaccgt ttaatgcagt
    40381 tattcaataa cttactcgaa aacagcctgc gctacactga tagcggcggc agcctgaaaa
    40441 tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg
    40501 tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa ggttcccgca
    40561 accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac
    40621 ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt acagtagagt
    40681 taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac
    40741 accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct
    40801 gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac tgccgtatgt
    40861 gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca ccgatggcct
    40921 gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg tgacggcaaa
    40981 aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt atatctgtaa
    41041 gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc
    41101 gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg aaggtcgttt
    41161 tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgctgaa
    41221 aacgctctct catgaaccag gaaaagtgtt ctcccgcgag catttgctca atcatcttta
    41281 tgacgactac cgcgtagtaa ccgaccgcac catcgacagc cacattaaaa acctgcgccg
    41341 caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgttggtta
    41401 ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact ttgttagccg
    41461 gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactgacat
    41521 tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt aaacagcacc
    41581 tttagatgct gttttcgata cgcaatttca atcaaggagt cattatggct ggttggtttg
    41641 aactcagtaa gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc aatggtgaga
    41701 ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg
    41761 tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt aacggcaaat
    41821 tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag atgtacgcca
    41881 ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca agccagacgg
    41941 tgaaagacaa tacgtaatta cgatgccggg cgtggggtgt tggcgtccgg ctttatatcc
    42001 aaataacgct tagttattaa taattaagag taaccgccgc cattaaaata gtgggcactg
    42061 acgagcactc gttgtgcttt ctttgtttac catcccatac ttatcctcct gaacatgttc
    42121 atgtgccaaa atatcttcta atactttcgt gacgctatcc ggtttatgac atacactgat
    42181 gtaagatttt tacttacagg cgggcgtaaa atggccagaa cgatgacggt tgatctcggc
    42241 gatgagttgc gtgaatttat cgagtcctta attgaatccg gcgactatcg cacccaaagc
    42301 gaagtgatcc gtgagtcact gcgattactt cgagaaaagc aagcagagtc tcgcctgcaa
    42361 gcgttacgtg acatgctcgc agaagggttg agtagtggtg aagctcagcc gtgggaaaag
    42421 gacgcgttct tacggaaggt gaaagcaggg ataaggaaat aatgtgcgta ttattaaact
    42481 catgccaaaa gccaatgagg atttagaagg catctggtat tacagctatc accatttcgg
    42541 cgaaccacaa gccgacagat atgtggaaca tctttcagac gttctccaga ttctgagtaa
    42601 caataatata ggtacgccca gacctgaatt aggcgaaggt atttttgttc ttccttttga
    42661 acgccacgtc atttatttcc tgcaatcacc aggggaaatt atcgtcattc gtatccttaa
    42721 ccaaaaccag gacgccacca gacatttaca ctggagctaa acccgccgat tgcgctacaa
    42781 tgcccgccct taaagtgggg gcactcccct aaccgcttca tcaggtgaag cggatctgac
    42841 ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg cgggaacgct
    42901 gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg
    42961 ttattccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc tcggcatcaa
    43021 tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc
    43081 caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc cggatgcgct
    43141 gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg aaatgccgat
    43201 ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg gtgaaattct ggcagcaaat
    43261 gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg aagagatacg
    43321 caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcgctgt gcatggccta
    43381 ctccggtcgc tgcctgctct ctggctatat caacaagcgc gacccgaacc agggcacctg
    43441 caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg atgttggcaa
    43501 catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg
    43561 cgcaccaacc gacaaagtgt ttatgatcga agaggcccag cgtccgggcg agtatatgac
    43621 cgcgtttgaa gatgagcacg gcacttacat catgaactcg aaagatctgc gcgccatcgc
    43681 ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag gtcgtaccaa
    43741 atctttctac tattgtgcac gcaccgcaca ggtttaccgc aaagctatcg atgacgccgc
    43801 tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg
    43861 ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact acgaatacgg
    43921 ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc gcaaggggga
    43981 cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg agctgatgac
    44041 gccgcaaggc aacattaatt ttacccttga gcacatggaa aacgccaaag gcgaagctat
    44101 gccgatagca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg atcttgagct
    44161 caattacgcg ctgctgatgc gtaatttctc cggggaaacc acgcgtaatc cccacggtaa
    44221 gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat cacatacagc
    44281 tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa atgctagctg
    44341 taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat tttttatgca
    44401 taagccctat ccctggtcac cgtcttccat tgaccacatc gatagaatct cccttcatag
    44461 cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag cacaaccaga
    44521 acgcgcatgt ttgacgctta ccaaaaaata ttctcactct ccacatttga atgtcagacg
    44581 agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca tttttttgcc
    44641 ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac cagatattct
    44701 gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat
    44761 attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat gcacagcaca
    44821 accagacata ataaaaatac taattaatga tatagcagct atctttttca tctcaccttc
    44881 ccccattaaa taccaacgac actctctagt gtttaaatat aataatggca tgattattat
    44941 aattgaatag gattataata aatgttctgt acaacatttc ctacataagt aggaattacg
    45001 gacattgagg cccttcaggg taactccatg ggggctttaa tatatttgga atagtatatt
    45061 taagatgcca ctgtttggtt gaatattagg catatgctct tttttgaaat ttatcggtgg
    45121 cagccgttag tattcgctgt ccccattgca agctcctggt ggtaaccact gaatcctcca
    45181 tacttgaact gactttttat cctccgactt tcatcctgtt ctgactccac cttttgtttt
    45241 ctgctctaca ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc
    45301 gccagcttac tgattcttaa tggcaaaagt actgacaatc tacccttgcg cgaagcaatt
    45361 atgctgttgc gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggcgat
    45421 gccgcacgat atgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt
    45481 ggcgatggca ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata
    45541 cccgcgctgg gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt
    45601 cctgaggcac tggataaggc gctgaaactg gcaattgccg gtaacgccat tgcgatagat
    45661 atggcgcagg tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg
    45721 cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc
    45781 attcatggct taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa
    45841 aactttcact ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggccggt
    45901 ggcggtcagc aattgtgtcc gaacgcgtta attaacgatg gcttgctgca actgcgcatt
    45961 tttaccggcg atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac
    46021 ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact
    46081 tttaatcttg atggcgaacc gttgagcgga caaaactttc atattgaaat acttccggcg
    46141 gcgttgcgtt gtcgattacc accagattgt ccattattgc gttaatcaaa ctattctctc
    46201 ttgaaccagg gagaatccga aatgccggat gcggtgtaaa taccttatcc ggcatacaga
    46261 acaatactac accgtaatta attttacccg tgtcttctgc aatgccagtt tatccacctc
    46321 tgatacagca tcatcggtaa taataatgtc aattttttcg attggcagta cctgattaaa
    46381 accgcgacgg ttaaatttcg atgaatcgag aacggcaatc actttattag cggcggtgac
    46441 catcgcaccg ctaatcgaat aaccttcatt aaaggtcgta ataccattaa ccgcatcaat
    46501 accatcagca ccgacaaaca ttaaatcggc attaatatcc tgcagggaac gttcagcaat
    46561 agatccatgc atagatcgcg ttttatggcg gaccgtgcca ccgcagacga ccagagtaat
    46621 gtctttattt tcggaaaggg caaacgcagc cgggagactg ttggtaatca ccgtgatatt
    46681 tttggcggtc attaatcctt cggcgataag cattgtggta cttccgctgt cgagaataac
    46741 ggtcatccct tcatggatca ttttgactgc cgcctgcgct attctgtttt tcggcgcgct
    46801 ggcgagctga aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccgga
    46861 cattattttc gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg
    46921 caaatcggca cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag
    46981 cacggttccc tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat
    47041 cgtcatttac ccatattcaa ttgtggctag tgtaaacgaa gggggaccat tggtgaatga
    47101 tctgttttgc acaataccgg gccagcatca cgctggcccg cggtttcagg gaatgagcaa
    47161 cactttgccc ggcatagcat tacgagcgat gtcacgcacc gcctgggcga agctttcaaa
    47221 gcttccacgg tgagcgatta atggctccag gcttaactta cgttctgtca gcaaccggct
    47281 cgccgtttcc cactcctgcc ccggccaagg gctggagtag ttcatccaac tgccgataac
    47341 cgtcagctct ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg
    47401 caacgtgccc accagcgcca gttgggcatg aggaccggca atctctaccg ccagttcgac
    47461 agtttgcggt acgccagccg tctcgaggat aagctgatta aagcgcagtt cgcgtaaaac
    47521 gctctgcatt tgcggcgcgc tcatttcgct actgttaaat gtttgcatcg caccgaaaga
    47581 ttttgccagt gccagttttt ctgaactaat gtcgatcgcc gtcacactct ttgctcccag
    47641 cgcgacagcg cactgaatgg ccagcaggcc aatggttccg gcaccaataa taataacgtt
    47701 tttattctca caaccttgcg ctaaatgaaa agcatgcagg ccaacggtaa tcggttcaat
    47761 aaaagcccca tcctcaatag gcatatcagt aggtagagca aagacatttt tccgcttaac
    47821 gacaatatat tcggcaaatc caccatcacg ccgtgagcca ataaaatcat attttgcgca
    47881 ctgggaataa aaccctttca aacactctgg acaagtaaaa cagggtaata acggcacaca
    47941 ggcaaccgca tcgccaggat gtaaatcatc aacaccggat cccaccgcat caatatagcc
    48001 gctaaattca tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg
    48061 taaatcggaa ccacataagc ccgagctggc aatttttacc cgcacctcat cctgatgttt
    48121 aatttcagga atgacgcttt ctgcaacgcg cacgatacca tcagtatcat tcaccactga
    48181 tttcataaaa actcctgatt gttaaggggg ataacctccc cctcaggtaa aaattattct
    48241 gcgagaacga ctttctcttg tttaataaag ccacgcgctc tacgccaggt catgaaaata
    48301 ccggtaagat aaattgcgcc gataataatg aatccgggaa tattttgcgg ggagaaaacc
    48361 tgaatcagta accaggtaat gggagaaccg ccctgatcca ttgaagccac catacccccg
    48421 gcttttaacg ccccagcatt agccgccagt tgggtgtgca aaccaatagt ttgcgtcgcg
    48481 atccacagag tgatgctcat gatgatgaca cccgagatta aggtgcggaa cagatttcca
    48541 cgatgcacgg cgactgccat cgccacaaag aagccgatgg tggcaagatc gccaaacggc
    48601 agcacctgat tacccggaac acaaacagca attaaaatgg tgagcgggat aaaaatcagg
    48661 cttgccgata ccaccgccgt atgccccagc agtaatgcgg gatccaggcc aatcaggaac
    48721 tcctgaccgc cgaacttcgc ctgtaaacga ctacgggcct gcttagcgat gggcgttaaa
    48781 ccatccatga tgggtttaat cacccgtggc attagcagca tcactgccgc cgtttttacc
    48841 gccagctgca atacaccttt gacatcgtaa cccgcgagga tgccgataat cagccccatc
    48901 acaaaaccca cggtgacagg ctcgccaaat ggaccaaatt tgcgctgaat atcgtcggcg
    48961 ctaaatttaa ttcggttaac gcctgggatt ttctcgatga tagcatcgac cagcaccgca
    49021 atcggcccca tatacgccga cgtaccgtgc ggaatagcaa taccttccag ctcaaagaaa
    49081 tttcgggtat cgcgggcaaa ccagtcgccg agcttataaa caaacgccgc gtgaattacc
    49141 acacctgcca tccctatcat ccatgaaccg gttgccaggt gcagcaacgc gccggtgaag
    49201 gtcatatgcc agatattcca gatatcaaca tttaccaccc gcgtcatacg ggtcagtaac
    49261 atcgccacgt taaccaatat cgcaatcgga atcgccacca gcgcaatttg cgacgcccag
    49321 gtcattggtg aagagcccgg ccagccgaca tcgaccacat gcagattcag gtcgaaattt
    49381 tccgccatcg ctttcgccgc cggaccaatg gaatccagca ttaagccaat cacaaggcca
    49441 atgccaacaa agccaatccc gatatgcaga cccgctttaa agcaatcgcc tgccttcatg
    49501 cctaatattt tagaaaaaat aatgatgaca atcggaagca tcaccgtagg gccgaggtcg
    49561 agaatataac gcatgacttc tgaaaacata ggtcacccct gtaagatagt cagaatttta
    49621 ttttgtaatg cttcgatacc gacaccagaa ataaaaggca tgccgtgaac taacggaata
    49681 tcgccaaaac tacgatccac tttggcagtg gtgcatatca aatgcacacc atccatatag
    49741 gtttctattt cattaacccg acactggatt aattcaacag gaatattatg attctgacac
    49801 aactctttaa tttcttccgc cgccatcgta gaggtcgcaa ccgcgcctcc gcaagcgaca
    49861 ataatcttgc gtttcataaa tagtcctttt tattgttaca gagggattta ttcgtctgaa
    49921 tctaaaacat actttgtgaa gtattccttt aactgagttt caggaagagt aattagtgtc
    49981 tcgacgatat cgggctgttg taacttgcca aataaacagc gtaaaagttt caattgctgc
    50041 tgcggatttt ccacaatcaa cgcaataacc aacgacacct ccacgtcgtt atcatcatcc
    50101 gcttgctgaa aatgaacttt atttgttggc cttaacagat aaatggctga cgacttagca
    50161 tgaatcgcct cacaatgcgg tatagcaatg gcgtgctgct caagcattat cccggtaggg
    50221 aattctgctt ctctggcaat taatgcctgt ggccaggtat cataaaccac acctttcgcg
    50281 agcatctcat taccgatatg ggttaaaact tcgctacgat cgacaaaaga aattccgcta
    50341 cgaacaaaca ggttagtcat ataccgtcct tattccgcac agccgtagcg ataggcgcgc
    50401 aaaacatcat aaattttatc cataatcagc tgatgcggta ttgctgataa ttccccggac
    50461 tgaatgcgtt caaattgttt gggaagatac tgactaatca tgcccagtgg gatgtccacg
    50521 ccttcaagat tcaccatcat cgtttcgacg ctatttttaa tccgactatg cggccagtaa
    50581 taacgaatac gatccgacag gctgtaacga atatccagta atgaatcgtt aaaacccgta
    50641 cgataatatt ttttccagta ttgcggttcg tcgagcatca cttcttcaat taccgccagg
    50701 caaccgctgc gattttcagg ggcgataagt tcctgctcaa tttgtgccag tgcaaatatc
    50761 gcctcgcgta aagcaaaggt taatgcggga ccgactttca atattgcaaa gtgatcgcgg
    50821 actaattccc aataagccgt ccgggtctgg taatcggtag aatgtgcttc ataaaccatt
    50881 cgggtgcttt ctatccattg cgccagcggc tgcgcttcct gcggctgata atggataata
    50941 ttgctgtgat caaattccac gcccggctgc accacgatgg caatcacgcg tgttaacgcc
    51001 tctgtcagcc cacgggcaat aaaggccttt tgatgcgtac gtaaagtatt ggcggcatct
    51061 tcaacatggg tgatgtgtac tgactgaatg gcgctggcct caccgcccgg aaccggaact
    51121 tcggtgccaa tgacatagct cagttgctca cgctggcaat ctgtcgccac actttccgca
    51181 gcaaagcaaa gcacagcagc tcgttccgca accgtttctg gtgctaacgg tatgggatcc
    51241 cccgcgcagg acattgacgc atcaagatga attttactga aaccggcacg aacatattcc
    51301 tttaccagct cgacggattt ttccatcgcc gcatccgcat tttcttgctg ccagcagttt
    51361 ggccccagat gatcgccgcc gagaataatg cgttcgcgtg caaacccaac tttatcggca
    51421 atcgtaaaaa caaattcgcg aaagtctgcc ggtgtcattc cggtataacc gccaaattga
    51481 ttgacctggt ttgacgttgc ttcaatcagc actttgcgcg tgctgttgcg atcaaatgcc
    51541 agcgccgctt cgataaccaa cggatgggca gaacagactg aacatatgcc gatatgttca
    51601 ccagctttat gccgggcaat taacgttttc atgttttttc cttattaaat ggcaggtgcg
    51661 ttatgccctg ccctcgcagc cacaatcggc aatcactttg ctcaccacat cgcgcattgc
    51721 ggatttagcc gactgcaaat aatcccgggg atcggtcgct tcagggtgct cggtcaggta
    51781 attttttaac gcctgcgaga aggcattttt cagctccgtt gcaacgttga ttttgcatat
    51841 ccccagtttg atggtttgct gaatatcctt agtcgataac cctgacgcgc catgcagcac
    51901 cagcggtaag ttcacccact ggcgaatgtt ctccagtcga gaaaaatcaa gcgccggtgc
    51961 gctggcatac atcccatgag ccgtgccgat ggcgaccgcc agggaatcaa ttccggttgc
    52021 ctcggcaaat tcacgcgcct gagcggggtt ggtataaaaa gcatcggctt cattgacttg
    52081 cacatcatct tcctggccgc caagttgccc cagctccgct tcgacgctga catcaaagcg
    52141 atggcaaaaa tccaccacct ctttgacccg tgaaatattt tgcgcaaaag gcaaatgcga
    52201 ggcgtcaatc atgactgagc gcacgccaga acgaaccttc tgagcgatat cgtcaaattt
    52261 cgtgtgatgg tcgagatgaa ttgccagtgg atggtgatat tgcttcgcca tcgcgctgac
    52321 cagcgccaac agactttctg taccagcatg agtaaatgtg ccaggcgttc cggcgatgat
    52381 gaccggcgca tgcaggttgg cagcggtttc caccaccact tgcatcgttt cgagattgtg
    52441 aatattgaat gccggaaccg cataaccgcc gcgctgtgcg ttgttcagca tctgctttgt
    52501 cgataccacg tacattttca tatcctgtcg tttgttttcg atttcaaaat ataatgaaat
    52561 gatttgtttt taaatgtcga gataacgatc acaaaaacga caatatgaaa attgttcgag
    52621 gagtaaaagg caaaaaaaaa cggcctcccg atagggaagc cgtagcaaag tgcgcgtgtt
    52681 tttatgccgg atgcggtgta aacatacgtg gcagtgctat gtaggcatga taagacgctt
    52741 ccgcgtcgca tcaggcatag gttgccggat gcagtgtaaa cgccttatcc gtcctacggg
    52801 gtggtgctgt tcgtatgagg cgtatcaggc aatagtaatt ttgctatcga gataaacgtc
    52861 ctgcacggca ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc
    52921 aagaatcagc cccattccgc cagcgcgttt gttgataacc gcagtacgca ctgcatcgct
    52981 gaggtcagtt tcaccgcccg cagcaccgcc ggagtttatc aacccagccc gacccatata
    53041 gcagttagct aactgataac gcaccagatc aatcgggttt tcgctggtca atttgctgta
    53101 aacacgatcg tcggtgtaac cgtaattaat tgctttatag ccgccgttat tttccgccat
    53161 tttttgtttg acgatatctg caccgatggt tgccgccaga tggtttgcct gaccggtcag
    53221 gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac gcaaataggc
    53281 ccacagcact gtcaccatac ccagctcgtg cgcacgttca aaagccgcag aaatttcttc
    53341 aatctggcga cgtgactctt ccgagccaaa atagatagtc gcaccaaccg caaccgcgcc
    53401 catgttgaac gcctgctcca cgctggcata cagcgtttga tcgtaggtat tcgggtaact
    53461 tagcgtctcg ttgtgattaa gtttgacgag gaatggaatg cgatgcgcat aacgccgcga
    53521 taccgacgcc agcacgccgt aagttgacgc cacacagtta cagcccgctt cgatcgccag
    53581 ttcaacaatg tttttcgggt caaagtagag cgggttagca gcaaatgaag ctccggcaga
    53641 gtgctcaacg ccctggtcaa ccggcagaat agaaagatat cctgtgccag ccagacgccc
    53701 ggtgttgtac aacgtctgca tattacgtaa caccgctggc gggcgattat tgtcaatcat
    53761 tacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa ttgtcataca
    53821 acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac
    53881 tatgctcccg taaattccga ttggatatcg gctatggatt gtcctggccc gccttttgcg
    53941 ggcaatcata atcctggtcg ttacgggcga gtttttccat cttttggatg ttttttcagc
    54001 gttttctgtt ggctcgattc atcagaaaaa atgttagcgc ggtcaagttt tcaccgcaaa
    54061 ggtatttaaa aggtattatt aagtggtatt gtcatcgcgt accttacatt acctgtcatg
    54121 aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg ttgaatggtt
    54181 tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg gttttagtgc
    54241 cggagaaatt ggctggtcgt atgcctgcac agccattgcg gcgatcctgt cgccaattct
    54301 ggttggctcc atcactgacc gctttttctc ggcgcaaaaa gtgctggcgg tattgatgtt
    54361 cgcaggcgcg ctgctgatgt atttcgctgc gcaacagacc acttttgccg ggttcttccc
    54421 gttactgctg gcctactcgc taacctatat gccgaccatt gcgctgacta acagtatcgc
    54481 ttttgccaac gtgccggatg tggagcgtga tttcccgcgc attcgtgtga tgggcactat
    54541 cggctggatt gcctctggtc tggcatgtgg tttcttgccg caaatgctgg ggtatgccga
    54601 tatctcaccg actaacatcc cgctgctgat taccgccgga agttctgctc tgctcggtgt
    54661 gtttgcgttt ttcctgcccg acacgccgcc aaaaagcacc ggcaaaatgg acattaaagt
    54721 catgctcggc ctggatgcgc tgatcctgct gcgcgataaa aacttcctcg tctttttctt
    54781 ctgttcattc ctgtttgcga tgccactggc gttctattac atctttgcca acggttatct
    54841 gaccgaagtt ggcatgaaaa acgccaccgg ctggatgacg ctcggccagt tctctgaaat
    54901 cttctttatg ctggcattgc cgtttttcac taaacgcttt ggtatcaaaa aggtattatt
    54961 gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta gtgcggatga
    55021 atatttcacc tacgcgttac tgttcctcgg tattttgctt cacggcgtaa gttacgattt
    55081 ttactacgtt accgcttaca tctatgtcga taaaaaagcc cccgtgcata tgcgtaccgc
    55141 cgcgcagggg ctgatcacgc tctgctgcca gggcttcggc agtttgctcg gctatcgtct
    55201 tggcggtgtg atgatggaaa agatgttcgc ttatccggaa ccggtaaacg ggctgacttt
    55261 caactggtcc gggatgtgga ctttcggcgc ggtgatgatt gccattatcg ccgtgctgtt
    55321 catgattttt ttccgcgaat ccgacaacga aattacggct atcaaggtcg atgatcgcga
    55381 tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg tgctctttat
    55441 gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccacg cagccgcgtt
    55501 aaagcacact ttggctggat tgaccgtttt ctgcctggac caaaggagaa taacgcggcc
    55561 tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg tctggcggat
    55621 gcgttactgg aacgtgaagg caagatcgat ccggatctga ttgggcgtaa tattctcgac
    55681 tgggcgctgc gtttcgacgc ctttaacaaa aacgtactag gtccgacctc gaagattgcg
    55741 cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaacaacgg cgtgaccaac
    55801 ggcgcagcga tgcgcgtctc gccattaggt tgtttgcttc cggcgcgtga tgttgattcc
    55861 tttattgatg atgtggcgct ggcgtcgagc ccgacccata aatccgatct ggcagttgca
    55921 ggtgcggtag tcatcgcatg ggcgatttct cgtgccattg acggagaaag ctggtcagcg
    55981 attgttgatt ccctgccttc aattgcgcga catgcacagc aaaaacgcat cactaccttc
    56041 agcgcctcac tggcagcacg tctggagatt gcgctgaaaa ttgtgcgcaa tgccgacggc
    56101 accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag cactattgag
    56161 tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa tcgctgcgcc
    56221 gtcctgtgcg ctaaccttgg cggcgacaca gacaccatcg gcgctatggc gacggcgatt
    56281 tgcggcgcgt tgcatggcgt taacgctatc gatcctgcat taaaggcgga actggatgcg
    56341 gtaaatcagc ttgatttcaa ccgctatgcc acagcgctgg cgaaatatcg tcaacaacgg
    56401 gaggcggtat gagcggcgct cgattacaca cgctgctgcc tgaattaacc tcgcgtcagc
    56461 cggtgatggt cgtcggcgcg gcggtcattg atgtgatcgc cgacgcttat gccctcccct
    56521 ggcgtgggtg cgatatcgaa ctgaagcagc agagcgttaa cgttggcggc tgcgcactga
    56581 atattgccgt ggcgttaaag cgcctcggca tcgaagcggg taatgccttg ccgctcggtc
    56641 agggcgtgtg ggcggagatt attcgcaacc ggatggcgaa agaggggtta atcagcctga
    56701 tcgataacgc cgaaggcgat aacggctggt gtctggcgct ggttgagccg gatggcgaac
    56761 gcacttttat gtcattcagc ggtgtggaaa atcagtggaa tcgccagtgg ctggcgcgat
    56821 taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc tcgccctgcg
    56881 gcgaattgtt agtggaatgg ctggaaaagc tgcaagacgt gacgccgttt atcgattttg
    56941 gcccacgtat cggcgatatc ccggacgcat tactggcgcg gatcatggcc tgtcgacctt
    57001 tagtatcact caatcgtcaa gaggctgaga ttgccgccga acgttttgct ttatccgcag
    57061 agataacaac acttggcaag caatggcagg agaaatttgc cgcgccgttg atcgttcgtc
    57121 tcgataaaga gggtgcatgg tatttcagca acgacgcttc tggctgcatt ccggcatttc
    57181 caacgcaagt tgtagacacc attggggcgg gcgacagtca tgcaggtggc gtacttgccg
    57241 ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca gtggcgtcgt
    57301 gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta ctcctcgcac
    57361 acaaaaacgt atagatcgct gcgacagtgg ctaatgctgt actcaatagg ccgttgctgt
    57421 tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa ctgaatatgt
    57481 gactgaaact cggcatccgg catccgggcg ctaacgcggg aacgcgtacg ttgtgggtaa
    57541 atgtgctggc tgcggaaata gtcataaagt gaaatcccga tggcatcaac atcatgaatt
    57601 aaatgcgccg gaacccacga ttcctcaatc gataccgctt cttcatcgac atagcgaata
    57661 cgcttaagca aaaagacatc acttcctgct tcgaccgcca gctgctgcgc gacttcttcg
    57721 ggacatttca cgacacgctt gttaacccat agcgtatcgg gctttttccc gcgcaatacc
    57781 acctgctgag aaaaacctcg cgcttctttc aacgagtatt cgaagatgtt gttgatctgc
    57841 gtgccgtaac cgcgcgaacg cgtcaccaca ccctcttctt ccagcgcctg catcgccttg
    57901 cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc gggcaaaata
    57961 ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc aaacttaata
    58021 taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat cagctgggta
    58081 tgcgcttgtt ccatttgtct ttttcctgcc ggggagtctg gtgggagtat atcactgctt
    58141 cttatttaag gcgtttcttt aatcccatca acgaatgcct gcaactcctc caccgtgcca
    58201 tgaaatacat taaaatccac cggaccattg atgccatcta cctgtccacg gtcggaatgc
    58261 tgccagaagc gccaggccat gccgtcattg tccggacgac gttgataata gtgagccacc
    58321 caccacggat attcattgac atagcccgcc agattggtgt gataaataac ggctcctgag
    58381 taaataatcg gcttttttcc cgtacttttt tcgaccattt ttagccactg acttactcgt
    58441 ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagtac cgcagggaaa
    58501 tcgccttgtg agaaatccac cgtttgcaga aataatcgtg cctgaaccgg cgctgctacc
    58561 gacggggaga aataatgata cgccccgcgc agcaggccat tttcgcggct tagttgccag
    58621 ttacgcgaaa aatacgggtc caccagcttt tcgccttccg tcgccttaat aaaagcaaac
    58681 tgtaagcgga taccgttgtc gcgcattttt gccacccgct gccagtcgat ccgctcctgc
    58741 cagcgagaaa catctattcc gtgaatggtg taactggcag gaatgcgaat agcgaaagat
    58801 ttaaccgggc gataaccgta gaaatggatg tagtctgtaa tctgaactgc tgtcgaatag
    58861 aaaaaattca ccgtctgacg cggataaatt gcaacgatgg aaattagccc aagagcgcat
    58921 aatagagagg tgagtttttt tcgactggta attcttagct gcattggttt catccctgaa
    58981 tgtcagtgcc aaaggctgac aataaccaaa gcagctatag tacggtgctc accaccaggc
    59041 gtggaagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg cctgggctaa
    59101 tgctgatgaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg tatggcgcgg
    59161 gcgtagtgca gccaacgccg cagagagcgt acaaccagtg ccgtgggtgt ttttggtcat
    59221 aatgcgcggt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt ccgggctttg
    59281 ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg ccaacagcga
    59341 tcgcccttgt tccagcattt cctgttcggt gcgcgcgtgt ggcgcgtcga gcaaggcggc
    59401 agcttcgggc aagtttggcg ttattaatga aacctgtggc aataatcgac tgcgcagcgt
    59461 agcaaccgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa cggtgtcgag
    59521 taccacgttt tggatctgat aacgttgcaa ccgttctgcc accgcttcaa caatatcggt
    59581 ttccgccaac ataccgattt tagtggtgtc gattcgcaca tcgctgaaca ccgaatcgag
    59641 ctgggcggcg acaaaatcag gctcaatgcg atacaccgac tgtacgccac gggtattttg
    59701 cgccaccagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga aggttttaag
    59761 atcggcctga atccccgcac caccactggg atcagtaccg gcaatcgtca gagcgttaat
    59821 tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa atgtggaaca
    59881 aaactgcctg gcccctcgct tctggcgact gcgcgctctc cggcttgttt catccagtga
    59941 caggcagatg cgacattttc cagcgtatcg cctggtaacg cacagcaggc agcgacaacc
    60001 gccgataatg cacagccagt tcctaccact ttggtcatta acggatcgcc accgtgaatg
    60061 ccaacggcgc gatggccatc ggtaacataa tccatctcgc cagtgaccac gacgattgcg
    60121 ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc gtcagtggta
    60181 tccactcccc gtccaccatt agcaatgcca gctaatgcca tgatttccga agcattacca
    60241 cgtatggcgg ttggcttatg agacagaagt tccagacaaa aacggcggcg ataatcgagt
    60301 gcaccaaccg ctactggatc aagcgtccag ggtgtttgag agctttttgc ttgctcaacg
    60361 gcggcacaca tcgactgtgc gcgtagctgc gtcagcgtgc caacgttaat cagcaacgca
    60421 ctggcaatag ccgcaaactg actggcctct tcggtttcga taaccatcgc tggcgatgca
    60481 ccgagcgcca gcaaggtatt ggcagtaaag gtttgcacca catcgttggt catgcagtgc
    60541 acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc tgaactcagc
    60601 aggtcgactt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc tggtggtccg
    60661 tgacttccct acgctggcat tatccagatc aggtgatacg ggtatttctc agccttcacg
    60721 cagaagggca ccccgagtcg tttggttgcg atgataaatc gcagaggagg atggtaatgt
    60781 ccagcgcacg cgttgtaaac gaggaaaaac ggcagtttta caatcgcgtt aataaattat
    60841 ttgatatatg aatccagcac cttcagaacg acatccagat cttcttcacg ttttagctca
    60901 tccccctggt gaacgatgtg ttccgtcaga tgacctttaa tcacttcccg catcagaccg
    60961 tttaccgcgc cacggatagc agcaatctgt tgtaaaactg cagcgcattc gtgcggttcg
    61021 tcgagcattt tcttgagcgc cacgacctgg ccctgaatct tactggcacg cgctttcagt
    61081 ttctgtttat cacggattgt atgagacatg gcaacacctg gttaacaaga atatgaaaaa
    61141 tcatagcact attaatctac tggggggtag tatcaggtac tggggggagt agaatcagat
    61201 tgccgaatta atactaagaa ttattatcat gaccgaattt acaactcttc ttcagcaagg
    61261 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
    61321 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca
    61381 ggcggtgatg ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttgattgc
    61441 ctttggcggg atggtggtca gcaagcgctt tactgctcaa tcagcagaac cgtggctcca
    61501 gttgatttcc gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggca
    61561 cggcgagcgc aactggctgg agaatatgca cgagcatgat catgagcatc atcatcacga
    61621 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
    61681 tgctcatgca cgagcccatg ctaatgacat taaacgacgc tttgatggta gagaggtcac
    61741 caactggcaa attttgttat ttggcttaac cggtggcctt atcccctgcc cggcagcaat
    61801 taccgtgctg ttgatatgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
    61861 cagtttcagc attggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag
    61921 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
    61981 tttttccagt ctgttgattg gcttagtcgg tgtgtatatg ggcgtacatg gcttcatggg
    62041 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
    62101 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
    62161 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
    62221 tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
    62281 aaaaataaga tgttgctggg tgcgcttttg ctggttacca gtgccgcctg ggccgcacca
    62341 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag cagtttcatt
    62401 gctgacttta agcatttcaa accaggcgat accgtcccag agatgtaccg caccgatgag
    62461 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
    62521 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
    62581 tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg
    62641 cagacacctt gttatttttt attattagcc acttgctcgt cttgcttgtt attagtcgta
    62701 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
    62761 gacgtagtaa ctggcagtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
    62821 cgcaatttct tttaaatttg tgtatcgttt actgctgtta gtgatattta aaagtgtgac
    62881 tgacgaacca ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcagtat
    62941 tgtgttgctg ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt
    63001 ttgattttgt tgacctaata aatttttaca acgcagaata atatcccctt gtttactatt
    63061 tattgttgct ttgttacctg tggctggtaa ttgtgaaacg ggaatactgt ctaaagtaat
    63121 taataaatcg tccggtttgc aggttgtggt tatcgggttg tatgacaatg tcagattaaa
    63181 ggaatagaca cctgcatttt gtgccaatgc atttctgtag ctatttacac atgcatccca
    63241 tgacttgcca cctaataaac acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga
    63301 tgaagcactg gataatgtcg caactccatt aatcatcact gaaccgccgc cccccgcaga
    63361 aacattcact tttgcattac tggccgcagg tgtcaccgtt accgaataac cactggtgaa
    63421 actggtctga ttatttgata acgtgatgtt gtttttattg gtccattcaa aatgtaaatt
    63481 taatttaatg gtatcgttat aaagtccaac aataatatcc gacgggctca taggggtgat
    63541 agtatttgta ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ccatttgtgt
    63601 ttgagtaagt gcaaaatccc cctgaacaac caaattgtgc tgaacagata actcactata
    63661 acatgccgca tagctcacag gcataaccat catcattact ataaataaca ttatacgaat
    63721 attcataacc acctgactat tactggcaaa tatagtttct actttcatca atctctttac
    63781 cgaaggtgat tgtgcatgaa agtccttgtt gcttatcaat tgctacatta accgatggcg
    63841 gtacttcatt ggtgcgaata aataactggc ttccctgacc gacaacgcca atattatgac
    63901 catggatatc attgacttca taaccaaaca ttaatggttg cccatccgct cttaacgctt
    63961 ttataaacca gggcttgcgc tgatcggtat caaaattaac cagtacaacc gcgccgcgat
    64021 aaggggcggc aattttccgg ttgccacgta attctgcttc gctatcgctt tgcgacacat
    64081 ccagcatcag gtgattttcc cgataaggtg tcattccgtc gtataccacc actccattac
    64141 ggtttgttgt gcgatatttt tgcccattga cataagcatc tttaattcct ggcgcattca
    64201 tcacagcaaa cgtttcggaa agacggttcg ccagattaac gccccccgac caggcgacaa
    64261 tgccccctga aacactggct ccagcctgtc gataagtact cgactgacta taactgccat
    64321 tcactgtcgc aaccggcgcg ttccaggtca aattcgcccc agctgtcgtt tcatttccct
    64381 gattctgata actcaggttg accccatagt taaactgatc ccggcttcca acggttcctg
    64441 ataatcccgt attatttgag gcaaacccct gatcatcaaa cgtcgttgag ttagacatat
    64501 atatttgccg acgaggcgtc gtaacgtcat caccccaatc aaagggaatc gatataaaaa
    64561 tattaaaacg tttctcttca tgatgattct cgtcatacgc ctggcttgcc gcgaggatat
    64621 agcttatccg ccgccagtta ttggaataac tcaactgata atccttactg ctgccgctac
    64681 gcccccagta atctcgccat aacgtactta aggacacaga cccccagcct tctggcaatg
    64741 actggctcat attggcagag aagctatttt tacggccaaa atcgttctgg taataatcgg
    64801 caatgtcata gacatcgttt tcatcacggc gataattatc tttattgttt gcccaaacgt
    64861 gatcattaaa tgtccggtaa tcacgcgatg aataacgcca ggccgccaga ccaaaacgcg
    64921 tcgacgtttg gctcacaaat ttgttgtagg caatttgata actttgcccg tcaaacacat
    64981 cgccgttgtc ttgtttacta tgcgacttcg tggcatcgac ggaaatggca ccaatgcgtg
    65041 tattccagcc agtccccaaa gtaaacgcgt aataattatt cgcgaccatc gagccaccat
    65101 acagcgtcaa taaattatta aaaccatact gataacccgc ctggacaaaa tcactttgtt
    65161 tgctcgcccc ttcaatatgg ctacgacccg ccgcaaaatc atatttcgat acgccgggtt
    65221 gcagcatatt tggcactgct gcataaggca ccagataggt ggttaccgag ccgtccgcct
    65281 ctttcacgct gacatcaaga tctgctccac caccggccaa ctgcaaatct gtaatcgcga
    65341 acgggccagg aggaacctct ttctgataaa ccacaaaacc gttctgttca atagttacca
    65401 gcgcattact ctgagcaatc ccctgcaccc gtggcgtaaa attttgtttc gagttaggca
    65461 acatctgcat atcacgaaac aaccgcacac cgctgaagcg aacagaatca aaaatatcgc
    65521 ttgatgtgta catatcaccc acgcgaagcg tgccgagaag ttgggcaaat ccacgttcca
    65581 gatacagggt attgcttttc cacccccctg gattgttatt tgttttactg aaactggcat
    65641 cagaatgcag ttgccacccc agtaaattta acccgctgtt aaaacgtaca tatgtactct
    65701 tactattacc cgacgcttta tagtcactgt aatactgact cacataataa gaggtataaa
    65761 acgcattaat accccgctcc cagttttccg gtggaacata gccactttcc agttcttcca
    65821 cccaggcctg cgggacactg aaatcgagac gaaaaacccc gatatcccag gtatagctcc
    65881 caccctgaac aagttgctca aatgttaaac attgcttacc gctggcgaag ttatcgctat
    65941 taatgcctaa ccgcttgata acttctattg ataaacatgt ttcttgcggg ttgtctttaa
    66001 caataatctc atatttcccg cgccattgct tattgacata aatatcgatg tcatactgcc
    66061 ccggtaaggg ttgattatca tcaagacgaa tatttgcaac ctgctggtct ttcattccac
    66121 ctatcataaa atgggtatca aaggtttctt cagctgcata agcttcaatg ccgagcaata
    66181 acgctacgat tgctgatgca agtggggtca ttctcaacat aggcagctcc tgcaattaaa
    66241 ttttgtcact aatatagttg ccgtggtcat cgataatggt cagataccag ttatttgcat
    66301 tattactttt gacattaaca ctctgacttt ctaagggggc aatcataata gtttcataat
    66361 tgactttaac attattagct ttgacatccg aaatcgtcac ccaattagct gagtcatttt
    66421 ttatcaccaa tccattgcca ctgcgattta ccagtaactt tttaaaggtc gctttattta
    66481 ccggagcaat acccgctggc cggtaaaaca acttaattct gttttgcatc gcaaacttca
    66541 gtgcattttt gccttcttgc tctggactat ttggtggaat atccagaaca ttcagataaa
    66601 aaatgctttc tttattagtg ggcagtttat tcggcataat tttgattttt acttgctgcc
    66661 cggaatttgc ccctattttt gccactggtg gcgttaacat gaaaggaacc tgaatttttt
    66721 ctggtggtaa cgacgtatcg ccatcatcaa tccacgcctg cagcagcgaa gaacggtttc
    66781 cctggttcat caactgcacc ataacttctt tattttctgc cgggtaaata atgcgcgtcc
    66841 cgtagataac aattccggca tgtgcaggaa ggaccataga aaaaatgagt aaagataata
    66901 atcctttcat cttaatgcct cttaaattaa aaagccgcca tggggatagc ggccatgttt
    66961 attacctgga gaaacttaca attttattta ttaatcctgc gccacacgca taaccagaga
    67021 agataacaca tcacctgatt gcactgagtt atctgtcagt ttcaggtatt ttgctttaaa
    67081 cggaatagca tattgacctt ccgctgtttt ataactctca atgttaaaag gtgcagaaac
    67141 atccaggaca tcgctgttat cacctaaata gagatagtaa cccacggtat cgttgctact
    67201 ggctatcgcc ttatcgttac cggttatttt atcgttggta atcaacgaga gttttacacc
    67261 agcaacttct ttcgggcagc taatttttaa catgctgctg gttacaccga ctgcatcatc
    67321 ataggtagaa tatttagctt taccttcaaa gacagtggtt ggtacagtcg gcatattaat
    67381 acttactgaa tcaccacccg cttcgaattt acacggagat tctgcaatat tccccttaat
    67441 agttaatgtt ccggtatcaa cgtctgcagc aaatactcca gcgctcataa aaaagaagaa
    67501 aagacagcgg cagcaataat tgaacgtttc ataatatgtt ccttaataa
//
LOCUS       DAZUFB010000012        66077 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZUFB010000012 DAZUFB010000000
VERSION     DAZUFB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.012"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..503)
                     /gene="folK"
                     /locus_tag="ACHY3M_000926"
     CDS             complement(24..503)
                     /gene="folK"
                     /locus_tag="ACHY3M_000926"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFS4315887.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(500..1897)
                     /gene="pcnB"
                     /locus_tag="ACHY3M_000927"
     CDS             complement(500..1897)
                     /gene="pcnB"
                     /locus_tag="ACHY3M_000927"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFS4315888.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1957..2853)
                     /gene="gluQRS"
                     /locus_tag="ACHY3M_000928"
     CDS             complement(1957..2853)
                     /gene="gluQRS"
                     /locus_tag="ACHY3M_000928"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFS4315889.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2920..3375)
                     /gene="dksA"
                     /locus_tag="ACHY3M_000929"
     CDS             complement(2920..3375)
                     /gene="dksA"
                     /locus_tag="ACHY3M_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFS4315890.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3553..4257)
                     /gene="sfsA"
                     /locus_tag="ACHY3M_000930"
     CDS             complement(3553..4257)
                     /gene="sfsA"
                     /locus_tag="ACHY3M_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4315891.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4272..4802)
                     /gene="thpR"
                     /locus_tag="ACHY3M_000931"
     CDS             complement(4272..4802)
                     /gene="thpR"
                     /locus_tag="ACHY3M_000931"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFS4315892.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4876..7304
                     /gene="hrpB"
                     /locus_tag="ACHY3M_000932"
                     /pseudo
     CDS             4876..7304
                     /gene="hrpB"
                     /locus_tag="ACHY3M_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
     gene            7436..9970
                     /gene="mrcB"
                     /locus_tag="ACHY3M_000933"
     CDS             7436..9970
                     /gene="mrcB"
                     /locus_tag="ACHY3M_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFS4315893.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10190..12433
                     /gene="fhuA"
                     /locus_tag="ACHY3M_000934"
     CDS             10190..12433
                     /gene="fhuA"
                     /locus_tag="ACHY3M_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFS4315894.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12484..13281
                     /gene="fhuC"
                     /locus_tag="ACHY3M_000935"
     CDS             12484..13281
                     /gene="fhuC"
                     /locus_tag="ACHY3M_000935"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFS4315895.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13281..14171
                     /gene="fhuD"
                     /locus_tag="ACHY3M_000936"
     CDS             13281..14171
                     /gene="fhuD"
                     /locus_tag="ACHY3M_000936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFS4315896.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14168..16150
                     /gene="fhuB"
                     /locus_tag="ACHY3M_000937"
     CDS             14168..16150
                     /gene="fhuB"
                     /locus_tag="ACHY3M_000937"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFS4315897.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGVCVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16185..17465)
                     /gene="hemL"
                     /locus_tag="ACHY3M_000938"
     CDS             complement(16185..17465)
                     /gene="hemL"
                     /locus_tag="ACHY3M_000938"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFS4315898.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17631..17678
                     /gene="yadX"
                     /locus_tag="ACHY3M_000939"
     CDS             17631..17678
                     /gene="yadX"
                     /locus_tag="ACHY3M_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFS4315899.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17690..19111
                     /gene="clcA"
                     /locus_tag="ACHY3M_000940"
     CDS             17690..19111
                     /gene="clcA"
                     /locus_tag="ACHY3M_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFS4315900.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19135..19200
                     /gene="yadW"
                     /locus_tag="ACHY3M_000941"
     CDS             19135..19200
                     /gene="yadW"
                     /locus_tag="ACHY3M_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFS4315901.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19193..19537
                     /gene="erpA"
                     /locus_tag="ACHY3M_000942"
     CDS             19193..19537
                     /gene="erpA"
                     /locus_tag="ACHY3M_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFS4315902.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19584..20207)
                     /gene="yadS"
                     /locus_tag="ACHY3M_000943"
     CDS             complement(19584..20207)
                     /gene="yadS"
                     /locus_tag="ACHY3M_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFS4315903.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20245..21045)
                     /gene="btuF"
                     /locus_tag="ACHY3M_000944"
     CDS             complement(20245..21045)
                     /gene="btuF"
                     /locus_tag="ACHY3M_000944"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFS4315904.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21038..21736)
                     /gene="mtnN"
                     /locus_tag="ACHY3M_000945"
     CDS             complement(21038..21736)
                     /gene="mtnN"
                     /locus_tag="ACHY3M_000945"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4315905.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21820..23337
                     /gene="dgt"
                     /locus_tag="ACHY3M_000946"
     CDS             21820..23337
                     /gene="dgt"
                     /locus_tag="ACHY3M_000946"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFS4315906.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23467..24891
                     /gene="degP"
                     /locus_tag="ACHY3M_000947"
     CDS             23467..24891
                     /gene="degP"
                     /locus_tag="ACHY3M_000947"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFS4315907.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNV
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25046..26203
                     /gene="cdaR"
                     /locus_tag="ACHY3M_000948"
     CDS             25046..26203
                     /gene="cdaR"
                     /locus_tag="ACHY3M_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFS4315908.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVVRHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26292..26678)
                     /gene="yaeH"
                     /locus_tag="ACHY3M_000949"
     CDS             complement(26292..26678)
                     /gene="yaeH"
                     /locus_tag="ACHY3M_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFS4315909.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVALKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26840..27652)
                     /gene="yaeI"
                     /locus_tag="ACHY3M_000950"
     CDS             complement(26840..27652)
                     /gene="yaeI"
                     /locus_tag="ACHY3M_000950"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFS4315910.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLHVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27707..28531)
                     /gene="dapD"
                     /locus_tag="ACHY3M_000951"
     CDS             complement(27707..28531)
                     /gene="dapD"
                     /locus_tag="ACHY3M_000951"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFS4315911.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28562..31234)
                     /gene="glnD"
                     /locus_tag="ACHY3M_000952"
     CDS             complement(28562..31234)
                     /gene="glnD"
                     /locus_tag="ACHY3M_000952"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFS4315912.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSYIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31296..32090)
                     /gene="map"
                     /locus_tag="ACHY3M_000953"
     CDS             complement(31296..32090)
                     /gene="map"
                     /locus_tag="ACHY3M_000953"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4315913.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32458..33183
                     /gene="rpsB"
                     /locus_tag="ACHY3M_000954"
     CDS             32458..33183
                     /gene="rpsB"
                     /locus_tag="ACHY3M_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4315914.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33441..34292
                     /gene="tsf"
                     /locus_tag="ACHY3M_000955"
     CDS             33441..34292
                     /gene="tsf"
                     /locus_tag="ACHY3M_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4315915.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAVLEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34439..35164
                     /gene="pyrH"
                     /locus_tag="ACHY3M_000956"
     CDS             34439..35164
                     /gene="pyrH"
                     /locus_tag="ACHY3M_000956"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4315916.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35456..36013
                     /gene="frr"
                     /locus_tag="ACHY3M_000957"
     CDS             35456..36013
                     /gene="frr"
                     /locus_tag="ACHY3M_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4315917.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36105..37301
                     /gene="ispC"
                     /locus_tag="ACHY3M_000958"
     CDS             36105..37301
                     /gene="ispC"
                     /locus_tag="ACHY3M_000958"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFS4315918.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDGASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37490..38248
                     /gene="ispU"
                     /locus_tag="ACHY3M_000959"
                     /gene_synonym="uppS"
     CDS             37490..38248
                     /gene="ispU"
                     /locus_tag="ACHY3M_000959"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFS4315919.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38261..39118
                     /gene="cdsA"
                     /locus_tag="ACHY3M_000960"
     CDS             38261..39118
                     /gene="cdsA"
                     /locus_tag="ACHY3M_000960"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4315920.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSSHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39130..40482
                     /gene="rseP"
                     /locus_tag="ACHY3M_000961"
     CDS             39130..40482
                     /gene="rseP"
                     /locus_tag="ACHY3M_000961"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFS4315921.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELLHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40512..42944
                     /gene="bamA"
                     /locus_tag="ACHY3M_000962"
     CDS             40512..42944
                     /gene="bamA"
                     /locus_tag="ACHY3M_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFS4315922.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43066..43551
                     /gene="skp"
                     /locus_tag="ACHY3M_000963"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43066..43551
                     /gene="skp"
                     /locus_tag="ACHY3M_000963"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFS4315923.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43555..44580
                     /gene="lpxD"
                     /locus_tag="ACHY3M_000964"
     CDS             43555..44580
                     /gene="lpxD"
                     /locus_tag="ACHY3M_000964"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFS4315924.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMSMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44685..45140
                     /gene="fabZ"
                     /locus_tag="ACHY3M_000965"
     CDS             44685..45140
                     /gene="fabZ"
                     /locus_tag="ACHY3M_000965"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4315925.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45144..45932
                     /gene="lpxA"
                     /locus_tag="ACHY3M_000966"
     CDS             45144..45932
                     /gene="lpxA"
                     /locus_tag="ACHY3M_000966"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFS4315926.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45932..47080
                     /gene="lpxB"
                     /locus_tag="ACHY3M_000967"
     CDS             45932..47080
                     /gene="lpxB"
                     /locus_tag="ACHY3M_000967"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFS4315927.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYSDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47077..47673
                     /gene="rnhB"
                     /locus_tag="ACHY3M_000968"
     CDS             47077..47673
                     /gene="rnhB"
                     /locus_tag="ACHY3M_000968"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4315928.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47710..51192
                     /gene="dnaE"
                     /locus_tag="ACHY3M_000969"
     CDS             47710..51192
                     /gene="dnaE"
                     /locus_tag="ACHY3M_000969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4315929.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51205..52164
                     /gene="accA"
                     /locus_tag="ACHY3M_000970"
     CDS             51205..52164
                     /gene="accA"
                     /locus_tag="ACHY3M_000970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4315930.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52263..54404
                     /gene="ldcC"
                     /locus_tag="ACHY3M_000971"
     CDS             52263..54404
                     /gene="ldcC"
                     /locus_tag="ACHY3M_000971"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFS4315931.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54461..54850
                     /gene="yaeR"
                     /locus_tag="ACHY3M_000972"
     CDS             54461..54850
                     /gene="yaeR"
                     /locus_tag="ACHY3M_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4315932.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKVFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54915..56213
                     /gene="tilS"
                     /locus_tag="ACHY3M_000973"
     CDS             54915..56213
                     /gene="tilS"
                     /locus_tag="ACHY3M_000973"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4315933.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56262..56522)
                     /gene="rof"
                     /locus_tag="ACHY3M_000974"
     CDS             complement(56262..56522)
                     /gene="rof"
                     /locus_tag="ACHY3M_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFS4315934.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56509..56709)
                     /gene="yaeP"
                     /locus_tag="ACHY3M_000975"
     CDS             complement(56509..56709)
                     /gene="yaeP"
                     /locus_tag="ACHY3M_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFS4315935.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56875..57420
                     /gene="yaeQ"
                     /locus_tag="ACHY3M_000976"
     CDS             56875..57420
                     /gene="yaeQ"
                     /locus_tag="ACHY3M_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFS4315936.1"
                     /translation="MALKATIYKATVNVADLDRNQFLYASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57417..57839
                     /gene="arfB"
                     /locus_tag="ACHY3M_000977"
     CDS             57417..57839
                     /gene="arfB"
                     /locus_tag="ACHY3M_000977"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFS4315937.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLSEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57853..58563
                     /gene="nlpE"
                     /locus_tag="ACHY3M_000978"
                     /gene_synonym="cutF"
     CDS             57853..58563
                     /gene="nlpE"
                     /locus_tag="ACHY3M_000978"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFS4315938.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58719..59542)
                     /gene="yaeF"
                     /locus_tag="ACHY3M_000979"
                     /pseudo
     CDS             complement(58719..59542)
                     /gene="yaeF"
                     /locus_tag="ACHY3M_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59596..61314)
                     /gene="proS"
                     /locus_tag="ACHY3M_000980"
     CDS             complement(59596..61314)
                     /gene="proS"
                     /locus_tag="ACHY3M_000980"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4315939.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIIEYL
                     VKQIKG"
     gene            complement(61426..62133)
                     /gene="tsaA"
                     /locus_tag="ACHY3M_000981"
     CDS             complement(61426..62133)
                     /gene="tsaA"
                     /locus_tag="ACHY3M_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFS4315940.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62130..62534)
                     /gene="rcsF"
                     /locus_tag="ACHY3M_000982"
     CDS             complement(62130..62534)
                     /gene="rcsF"
                     /locus_tag="ACHY3M_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFS4315941.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62652..63467)
                     /gene="metQ"
                     /locus_tag="ACHY3M_000983"
     CDS             complement(62652..63467)
                     /gene="metQ"
                     /locus_tag="ACHY3M_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFS4315942.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63507..64160)
                     /gene="metI"
                     /locus_tag="ACHY3M_000984"
     CDS             complement(63507..64160)
                     /gene="metI"
                     /locus_tag="ACHY3M_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFS4315943.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64153..65184)
                     /gene="metN"
                     /locus_tag="ACHY3M_000985"
     CDS             complement(64153..65184)
                     /gene="metN"
                     /locus_tag="ACHY3M_000985"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFS4315944.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65372..65947
                     /gene="gmhB"
                     /locus_tag="ACHY3M_000986"
     CDS             65372..65947
                     /gene="gmhB"
                     /locus_tag="ACHY3M_000986"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFS4315945.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDTMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15593 a        16476 c        18208 g        15800 t
ORIGIN      
        1 tttatattcg taatataaat aaattaccat ttgcttaatt tgtcaaatgc tcttgtatgt
       61 aagacttcac gcaacgtctc cccatcagga aacgccagct ccggtgcaat ctcaaacagc
      121 ggccacagca taaatccacg attcttcata tcgtagtgcg gaacggtcag gcgttcggta
      181 tttatcactt cattaccaaa cagcatgata tcgagatcca gcgtgcgtgg tccccagcgt
      241 tcagctttac gaacacgacc ctgttgcagt tctatgcgct gtgtatggtt gagtaactct
      301 tccggggcga gcgaagtttc aagcgccacg gcggcgttga ggtaatcggg ttgatcttgc
      361 ggccccagcg gtggggtgcg gtaaaacgaa gaaacggcaa gaatgtggct ttcagggata
      421 tcgcctaatg ctttcagggc agcattgacc tgctccagcg gagaggccag attgctgcct
      481 atggcaatat acgccactgt catgcggtac cctcacgacg cggtgcgcgt ttgcgtggac
      541 gacgagtacg gcgacgcggt gacggctctt catccaactc gttaagcatc cctttttgat
      601 ctggtggcgc ggaaacctgg aactcacccc accatttcac cagacgctgc agttcagcgt
      661 tacgttcaac ttcagctcgc aaggccaaca ggtcataagc cgcacggaac ttaggatgct
      721 ccagcagttt ccatgcgcgt ttaccctgac gacgggacat acgcaactgc aactgccaga
      781 tatcgcgggt taacgtcgtc agacgtttcg ggattgccag tgaacggcag gcttcgtcca
      841 gcacgtcgtt catcgccagc gcgaaagcgt cgtgataggt caggccgctt tcctgggcaa
      901 tcttctgtgc cgtctccagc agtgggtacc agaacatggc ggcaaacagg aacgccgggt
      961 tcacgcgcat atcgttatgg atacgcgtat cggtattctt cagcacctgt tcaatgatcc
     1021 gctccatcgg gctgtcgcca ttttccgtga agtagcgggt aatggtcggg aacagcggct
     1081 ggaacagatg atattcacac aacagcttat aggtatcgta accgtagccc gcttgtagca
     1141 gtttaagcga ttcttcaaac aggcgtgccg gtgggatatc gttcaacagg gtagcgaggc
     1201 gagggatcgg ttctgcggtt tccgggctga tgcgcatacc caatttggcg gcaaaacgta
     1261 ccgcgcgcag catacgtacc ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac
     1321 ggataacgcc gtccttcaga tccttcatac cgccaacgta atcacggacg gtaaaatccg
     1381 ctacgctgta atagaggctg ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg
     1441 agccgaaaat gttgtcgcgc agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt
     1501 cgctgacgtt accttcgtgg tgtccacgga aggtcgcaac ttcgataatc tccgggccaa
     1561 acattacatg agccagacgg aaacggcgac ccaccaggcg gcagttacgg aacagtttgc
     1621 gcacctgctc aggcgtggcg ttagtggtta cgtcaaaatc tttcggcttt ttgccaagta
     1681 acaggtcgcg cacgccgccg ccaaccagcc aggcttcgta tcccgctttg ttgagcctgt
     1741 acattacctt cagggcattt tcactgatat ctttgcggga aatagcatgc tgctcacgcg
     1801 ggatcaccgt cacctgtgga cgggcgactg cctgttcagc ctcgctttcc tcgcggctta
     1861 gcaccttgcg gcaaaaatta gcgactcggg taaaaatagt acacctcggt agtgtcaaac
     1921 atcattcagg acaaaaaaat agcggctaat catagctcag catgacgcat ttgagaatgt
     1981 tgaatttaca attgccgact cgggcacggc ggtaagccgc cagtttttga cggctgactg
     2041 aaggatttgc tcgacgctga aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg
     2101 aagtgccgcg attagtaccg ggcgtggatc gcctttcggc aacgcaggcg catgattctg
     2161 cttggaaagt ttagcgcctt gtggattaag cgccagcggc agatgaatgt aatctggcac
     2221 tttccagcca aaaagctggt acagcgagat ttgccttact gttggttcaa tcagatcagc
     2281 cccacgtact atttctgtaa cgccctggaa atgatcatca accacaacag ccaggttgta
     2341 ggcgaacaac ccatcacggc gatgaatgat aaaatcttcc cgtgccagtt tttcgtcggc
     2401 gtgaataatg ccgcgcagct ggtcagtaaa ttgcgtgacc ggatgctgct ggcggatacg
     2461 cactgcggca ttgtctggtc catgatgcaa cacccggcaa tgaccgtcgt aaataccgcc
     2521 aatgctttga atacgcgcac gcgtacaggt gcagtaataa cttagtcctt gttcatgtaa
     2581 ccaggcgagt gcttcacgat aggcgtggtg acgttgcgat tgccagagaa catcgccgtc
     2641 ccagtgcaga ccgtaatgtt ccagctggcg caggatagtt tctgcggcac cgggaacttc
     2701 acgaggcggg tcgatatctt ctatgcgtac cagccagcga ccttgccggg cgcgagcctg
     2761 caaatagctg ccgagcgcgg cgatcagaga gccaaaatga agctcgccgg aaggagaggg
     2821 ggcgaagcgg ccaatatact gtgtgtctgt catctctttg aacaaaaaat aaggcgggag
     2881 catttcccgc ctgtggtaaa cgtgatggaa cggctgtaat tagccagcca tctgtttttc
     2941 gcgaatttca gccagcgttt tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag
     3001 acggcgaata ccaatttcaa caccgcagga ttcgcagtag ccgaaatctt cgtcttccac
     3061 ttttttcagc gtcttctcga tctttttgat cagcttacgc tcgcgatcgc ggttacgcag
     3121 ttcgaggctg aactcttctt cctgggctgc acggtctacc gggtccggga agttggctgc
     3181 ttcatcctgc atatgtgtaa cggtgcgatc gacttcatcc ctgagttgat tacgccatgc
     3241 ttccagaata cgacggaagt gcgccagctg ggcttcattc atatactctt cgcccggctt
     3301 ctcctgatat ggttccaccc cagcgatggc gagaatactc agggacgatg ttttacggtt
     3361 ttgcccttct tgcatgttgc ttctccttaa cacgcactat cgatccccat gttcggggga
     3421 aaaatgaggc cgctataaat agcagatgct tttccggata gcaattatct aaacgtaaca
     3481 cttgacaact gtgtgaggaa aagcgtattt gcgcacgcga ccagaatgta aattaaccag
     3541 ttacttactt tactacaatg taaccggcag tgatttttta agagccatgc cttcagcaga
     3601 aatttccgct ttgtaagcca gaatttctac ccccctctgt tgagcttctg acaatagttg
     3661 cgcgtatttc tcatcgatgt ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac
     3721 ggcgaaaaag ataaccgcac gctggccttc agccgctacg ctcatcaact cccgaaggtg
     3781 tttctgacct cgttcagtga ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt
     3841 aaccgatttc acttcaatat agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat
     3901 acggctgcgt tctgcgccgt attttacttc gcttttcagc gagctatagc ctgacagttc
     3961 tgaaattgat tcattaagga tagcctcttt cgtcaacctg ttagcccaaa gcgtgttgac
     4021 gcaaataaat gcgccgctct ggctttgagt taattcccag gtgtgtgggt atttccgttt
     4081 ggtgttgtct gaagtcgaat accagacggt atcgccaggc gttgcacaac cggtcattgc
     4141 acccgtattc gggcagtgta gcgttaattc gcgaccatcg ggtgtgatca catcggctaa
     4201 aaaacgtttg taacgctgaa ttagcgtcgc gcgctgtaga gggggagaaa attccattgc
     4261 gacaatcctt gttattgcgt tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca
     4321 cgggcaaacg aggaggcgta aagggtgaac tccgtcaccg catacgacca gttaaaacct
     4381 ggcggcggga ttgtcaccgc ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac
     4441 ggacgattgc tttgaaaaca accgctgcgg gcagcctgtg aacggagcat attcgccagc
     4501 tggattaagc cgcgtggcgg ctgacgcatc cctaaccaca ccacacgcga acgcagccat
     4561 tgtccggcgt catcaagcgt gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga
     4621 gaaagcgcct tctctttctc ggcgctcact tcgcctaaaa atgccagagt cagatgcaaa
     4681 ttatcggcgg cgaccggacg tcccgcctca ggtgggaagt gtgtggcgcg ccagtggata
     4741 atctgttcgc ggatttctgc aggtaagtcg atagcaaaga acagacgttg cggttcagac
     4801 atgtgaggca ctcggttatg aattaccgcg atgctacaat gtggcgcgaa gaatgttaac
     4861 cctctggagc gttttgtgtc gtcgttgccc gttgctgccg tcttacctga attacttacc
     4921 gctctcgatt gtgcgccgca ggtattatta agtgcgccga ccggggccgg gaaatcaacc
     4981 tggctgccgc tgcaactgct ggcgcatccc ggcattaacg ggaaaattat cctgctggag
     5041 ccgcgtcgtc tggcggcgcg taacgtcgcg caacggctgg cggagctgct taacgaaaag
     5101 ccaggcgata ccgttggcta ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc
     5161 ctggaagtag ttaccgaagg cgtgctgacg cggatgatcc agcgtgaccc ggagttgagc
     5221 ggtgttggat tggtgatcct cgatgagttt catgagcgca gcttgcaggc ggatttggcg
     5281 ttggcgctgt tactcgatgt gcaacaaggt ctgcgtgatg accttaaact gctgattatg
     5341 tcggctacgc tggacaacga ccgcttgcag caaatgctgc cagaagcgcc cgtcgtcatc
     5401 tcagaagggc gctcgtttcc ggttgaacgc cgttatttac cactgcccac gcatcagcgt
     5461 tttgacgaag ccgttgcggt agccaccgct gaaatgctgc gtcaggaaag tggatcatta
     5521 ctgttatttt tacctggcgt cggagaaatt cagcgtgtgc aggaacaact ggcttcgcgc
     5581 atcggcagcg atgtattgct ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga
     5641 aaagcgatcc tcccggcacc gcaagggatg cgcaaagtgg tgctggcgac caatattgct
     5701 gaaaccagtt taaccatcga aggtattcgt ctggtggtgg attgtgccca ggagcgtgtg
     5761 gcgcgttttg atccgcgcac ggggcttacg cgactgatta ctcaacgcgt tagccaggca
     5821 tccatgacgc agcgtgccgg gcgcgccggg cgtctggagc cgggtatctg cctgcattta
     5881 atcgccaagg aacaagcaga acgcgccgcg gcgcaaagtg aaccggagat attacaaagc
     5941 gatctttccg gtttgctgat ggaattactg caatggggat gcagcgatcc ggcgcagatg
     6001 agctggctgg atcaaccgcc agtagtgaat ctactggccg cgaaacgtct gttacaaatg
     6061 ctgggggcac tggagggtga acggcttagt gcgcaagggc aaaaaatggc agcgctgggt
     6121 aacgatccgc gtttagcggc aatgctggtt agcgcgaaga acgacgacga agctgctacc
     6181 gcggcaaaaa ttgccgccat tctcgaagag ccgccacgga tgggcaatag tgacctgggc
     6241 gtggcgtttt cgcgcaatca accagcctgg cagcaacgta gtcagcaact gttaaaacgc
     6301 ttaaacgtac gtggcggtga ggcagacagt tcgcttatcg cgccgctact tgccggggcg
     6361 tttgccgatc gcattgctcg tcgccgtggg caagatggac gctatcaact ggcaaacggc
     6421 atgggagcga tgctcgatgc caacgacgcg ctaagccgcc acgaatggtt gatcgcaccg
     6481 ttattattgc agggcagcgc ctcgccggat ggcggatttt actggcgctg ccggtcgata
     6541 ttgatgagtt agtacaacgc tgcccgcagc tggtacagca gtctgacact gtggagtggg
     6601 atgacgcgca aggtacgctg aaagcctggc gtcggctgca aatcggtctg ctgacggtga
     6661 aagtgcagcc gctggcgaaa ccctcggaag acgagttgca tcaggcgatg cttaatggca
     6721 tccgcgataa agatttaagc gtgctcaact ggacggcgga agcggaacag ctacgcttgc
     6781 gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt gatgatgaaa
     6841 gtttattggc gacgctggaa acgtggctgc tgccacatat gactggcgta cattcactac
     6901 gcggcctgaa atcactcgac atttatcagg cactacgcgg attacttgat tggggaatgc
     6961 agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga agccggatcg
     7021 ccattcgtta tcatgaagat aacccgcccg cgctggcggt gagaatgcaa gagatgtttg
     7081 gcgaggccac caatccgacg atcgcccagg ggcgcgtgcc gctggtgctg gagttgcttt
     7141 cacctgccca aaggccatta caaatcacac gagatttgag cgccttctgg aaaggagcgt
     7201 accgtgaggt gcaaaaagag atgaaagggc gttatcccaa acatgtctgg ccggacgacc
     7261 cggcaaatac tgcaccgacg cgacggacga aaaagtattc gtaaggttgg ttttctccct
     7321 ctccctgtgg gagaggggac cgatcgagca caaattttga gagatatctt cttctgtctt
     7381 gtaacagaag aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc
     7441 cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa
     7501 ggtaagccgt cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga
     7561 tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa
     7621 acgcggctgg ctatggctgc tgttaaaact ggctatcgtt tttgccgtgc tgatcgccat
     7681 ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact
     7741 gcctgcggca gtttatggcc gaatggtcaa ccttgagcca gatatgacca tcagcaagaa
     7801 cgagatggtg aagctgctgg aggcgactca gtatcgtcag gtgtcgaaaa tgacccgtcc
     7861 tggcgaattt accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc
     7921 ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt gatggcgatc atctggcgac
     7981 gatcgtcaat atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat
     8041 caccatgatc tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc
     8101 tgatttgctg gtggatactt tgctggcgac agaagaccgt catttttacg agcatgacgg
     8161 catcagcctc tactccatcg gacgtgcggt gctggcaaac ctgaccgccg gacgcacggt
     8221 gcagggagcg agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagtgagcg
     8281 ttcttactgg cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag
     8341 caaagaccgt attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa
     8401 cgaaatccgc ggcttcccgc tggcgagctt gtattacttt ggtcgcccgg tagaagagct
     8461 aagcctcgac cagcaggcgc tgttagtcgg catggtgaaa ggagcgtcca tctacaaccc
     8521 gtggcgtaac ccaaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca
     8581 gcaacagatt attgatcaag aactctatga catgttgagt gcacgtccgc tgggtgttca
     8641 gccacgcggt ggggtgatct ctccacagcc agcctttatg caactggtgc gtcaggagct
     8701 gcaggcgaaa ttaggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt
     8761 tgactcggtg gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa
     8821 gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg
     8881 tgaagttcgt gcgatggtcg gtggttctga gccgcagttt gcgggctaca accgtgcgat
     8941 gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg acttatctga ctgccttaag
     9001 ccagccgaaa atctatcgtc tgaatacgtg gattgcggat gcgccaatcg cgctgcgtca
     9061 gccgaatggc caggtctggt caccgcagaa tgatgaccgt cgttatagcg aaagcggcag
     9121 agtgatgctg gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa atctggggat
     9181 ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca
     9241 gttgcatccg gttccggcga tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc
     9301 gcaggcgttc cagaccatcg ccagcggcgg taaccgtgca ccgctttctg cactgcgttc
     9361 ggtaattgcg gaagatggca aggtgctgta tcagagcttc ccgcaggcgg aacgcgctgt
     9421 tccggcgcag gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgcggtac
     9481 gggtcgtcag cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac
     9541 caacaataac gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg
     9601 ggtaggccgt gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat
     9661 ttatcagcgt tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga
     9721 tattgcagat atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt
     9781 cttgccggtc tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca
     9841 gccgtcaggc aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc
     9901 tgctcagcaa gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg
     9961 tagtaattaa catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca
    10021 tcccatctaa gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg
    10081 ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa
    10141 taatcattct cgtttacgtt atcattcact ttacatcaga gatataccaa tggcgcgttc
    10201 caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt
    10261 aagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt
    10321 taccgctgca cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg
    10381 acagtctgct accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc
    10441 tgttgtgacc gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag
    10501 ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat
    10561 cattcgcggc tttgcggcag aaggccaaag ccagaataac tatctgaatg gcctgaagtt
    10621 gcagggcaac ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat
    10681 tatgcgtggc ccggtttccg tgctttacgg taaaagcagt cctggcggcc tgttgaatat
    10741 ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga
    10801 cagcctgttc cagactggtt ttgactttag cgattcgttg gatgatgacg gtgtttactc
    10861 ttatcgcctg accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca
    10921 gcgttatgct attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt
    10981 cctttcttac ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg
    11041 aaccgttgag ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa
    11101 gaacaacacc tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa
    11161 cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag
    11221 cgtttatggt tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc
    11281 attagcgcca gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa
    11341 gctgcaaaac ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga
    11401 ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt
    11461 tggttacgac gactctgtgc cactgctcaa tctgtacaat ccggtgaata ccgatttcga
    11521 cttcaatgcc aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca
    11581 aacgggcgtt tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg
    11641 tcgttatgac tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg
    11701 tgatgacaaa cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac
    11761 accttacttc agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa
    11821 tattttcgca ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga
    11881 tcgtccgatt gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc
    11941 ggaccctgag ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga
    12001 aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac
    12061 cgatgcggaa tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa
    12121 acacatggct tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac
    12181 gctgggcacc ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa
    12241 agtgggaagt tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat
    12301 ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag
    12361 ctgctttaac acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac
    12421 cttccgtttc taatttctct tttggggcac ggatttccgt gcccatttca caagttggct
    12481 gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt
    12541 cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg
    12601 accggtctga ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggccgt
    12661 catcagcctc cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc
    12721 agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg
    12781 atgaccgtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc
    12841 tttggtgcgg cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg
    12901 ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc
    12961 atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat
    13021 atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg
    13081 acggtcattg ccgtgctgca cgatatcaat atggcggcac gctactgtga ttatctggtc
    13141 gccctgcgcg gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa
    13201 accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca
    13261 cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa
    13321 cggcgatggc gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg
    13381 accccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca
    13441 tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat
    13501 tgccggactc agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg
    13561 aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcacca gaaatgctgg
    13621 cacgtattgc gccgggtcgc ggatttaact tcagtgacgg caaacagccg ttggcgatgg
    13681 cgcgtaaatc gctgacggaa atggcagatt tacttaacct gcaaagcgca gcagaaacgc
    13741 atttagcgca ctatgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg
    13801 cacgcccgtt actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa
    13861 acagcttgtt ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa
    13921 ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcggcgtat aaagacgttg
    13981 atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc
    14041 tgtggcaggc catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt
    14101 tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag
    14161 gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgttggcgc tgttagtgat
    14221 tgtcgctacg gcgctaacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc
    14281 gcaggccgcc tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt
    14341 gttgccgcgt ctggcgattt cgctgctggt gggcgcaggc ctggggctgg tgggcgtgct
    14401 gtttcagcaa gtgctgcgta atccgctggc ggaaccgacg acacttggcg ttgctacagg
    14461 cgcgcaactg ggaattaccg tcaccacact ttgggcgatc ccaggtgcta tggcgagcca
    14521 gtttgccgca ctggtgggcg tttgtgtcgt tggcttaatc gtctttggcg tcgcgtgggg
    14581 gaaacggctt tcgccggtta cgcttattct cgcggggctg gtggtgagcc tttattgcgg
    14641 ggcaattaat cagctactgg ttatcttcca tcacgaccag ctgcaaagca tgttcctgtg
    14701 gagcaccgga acgctgacgc aaaccgactg gggcggcgtt gagcgtttat ggccgcagtt
    14761 gctgggcggc gtgatgttga cgttattgct acttcgcccg ttaaccctga tggggcttga
    14821 tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctc gcgcgtctgg cagcgctgtc
    14881 gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg
    14941 gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat
    15001 gctggcgtcg ctgattgggg cgctgatcct ctggctttcc gatcaaatca tcctctggct
    15061 gactcgcgtg tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct
    15121 gctactgtgg ctgttgccgc gtttacgcag cattagcgcg ccggatatga aggtcaacga
    15181 tcgtgtcgcg gctgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct
    15241 gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc
    15301 gagcggggcg ttgcttgagg atttgatgcc ctggcgctgg ccgcgaatta tggcggcgct
    15361 gtttgcgggc gtcatgctgg cggtggcggg ctgtattatt cagcggctga ccggaaaccc
    15421 gatggcaagc ccggaagtgc tggggattag ctctggcgca gcgtttggcg tggtgttgat
    15481 gctgtttctg gtgccgggta atgcctttgg ctggctgtta cctgcaggca gtctcggcgc
    15541 ggcggtgacg ctgttgatca ttatgatcgc cgccggccgc ggtggatttt ccccacaccg
    15601 catgttactg gcggggatgg cgttaagcac cgcgttcacc atgcttttga tgatgttgca
    15661 ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa
    15721 cgcgaccgat gcgcaggtct ggcgcacggg aattgtgatg gtgattttgc tggcgattac
    15781 cccgctgtgc cgccgctggc tgaccatttt accgctgggt ggtgataccg cccgagccgt
    15841 aggaatggcg ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc
    15901 gaccgcgacg atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc
    15961 gcggatgatg ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg
    16021 tttactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc
    16081 ggcagggctg ctgtcgacct ttatcggcgc gccatatttt atctatttgt tgagaaagca
    16141 gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa atcatcacaa cttcgcaaac
    16201 acccgtcgtg cagcatcgat ggtattattg atatcttcca tgctgtgcgc cacggacata
    16261 aagcccgctt caaacgctga cggtgccagg taaacacctt cgtccagcat catatggaag
    16321 aaacgcttaa agcgttccac gtcacaggcc atcacatcct gatagcacgt cacggactcg
    16381 gcgtcggtaa agaaaatacc gaacatgccg ccaacgtggt taacgaccag cggaattccg
    16441 gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt
    16501 tcgtgaacgc ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatt
    16561 gggttaccgg aaagcgtacc cgcctgatag accggacccg tcggggccag cgcatccatt
    16621 acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat tttgcccagg
    16681 caggtgagat ccggttccac accgtaataa tcctgtgcgc cagctagcgc tacgcggaag
    16741 ccagtcatta cttcatcgat gatcagcaat gcgccaaatt cgtcgcacag cgcgcgcaga
    16801 cctggcagga actctggcag cggtggaacg cagttcatat tgcctgccac cggctcgacg
    16861 ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga agccagatcg
    16921 ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacacccgg cgagtttggc
    16981 tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg
    17041 taacaacctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg
    17101 gcgctcatcg tcgcttcagt gccggagttc accatgcgca ccatatccat ggtcgggacc
    17161 agttcggtca ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa
    17221 ccacgctcgg cggcttcaat cacggcattg cggatagccg gatggttatg gcccagcacc
    17281 atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa
    17341 gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca
    17401 ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta
    17461 ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata
    17521 acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag
    17581 agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg
    17641 cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga
    17701 tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca
    17761 acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac
    17821 gcttgtcggg ctggcagcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg
    17881 tatgggggcg ctggtacata ctgctgataa ttatccgctt ctgttaaccg tcgcttttct
    17941 ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgccggaagc
    18001 aggtggttcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ccgttcgctg
    18061 gtggcgtgtg ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt
    18121 gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca tggtgcttga
    18181 tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc
    18241 ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat
    18301 gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat
    18361 gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact
    18421 ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat
    18481 tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca
    18541 cggcggcaat attaccaaat gggtactgat gggtggtgcg attggcggtc tgtgtggatt
    18601 gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac
    18661 cgcggggaat ttcagcatgg gaatgctggt gtttattttc gtcgctcggg tcattaccac
    18721 cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg
    18781 tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct
    18841 ggaggcgggg acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc
    18901 gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc
    18961 aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaaccgct
    19021 atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctatgcaac tggcgcgaag
    19081 caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg
    19141 attattattg ggttagaatt tgcccaattg ccgatgtcgt ttggagcaaa atatgagtga
    19201 tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat
    19261 cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag
    19321 cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga
    19381 aaaacagggc gttggcttgg tggttgatcc gatgagcctg caatatctgg tcggcggttc
    19441 cgttgactat accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa
    19501 aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa
    19561 actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc
    19621 ttaagatgcc aacgaatagc cgccagccga atcaagagcg tcacgaccat gcccatcata
    19681 ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg
    19741 ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatttc gcgggccaga
    19801 acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac
    19861 ggaccggctt ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca
    19921 acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg
    19981 atggtcagca tgctggttac cattgcaacg accagatcgg tgggatcttt cacccaaaat
    20041 accgggccgt gatccagcgc catgtcgcga attgtcccgc cgcctacagc ggtaaccacg
    20101 cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag
    20161 atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca
    20221 ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg
    20281 tgcagcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac
    20341 gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc
    20401 tgtaatgaca atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca
    20461 gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg
    20521 aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt
    20581 tttaggttta tcagcatatt gcgctttcaa ttgcgcgtac tgatccagca gggattgcgc
    20641 ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc
    20701 aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg
    20761 gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc
    20821 gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg
    20881 tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag
    20941 ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc
    21001 aagaaaagac agggcgacca gcgccctgaa cagtgactta gccatgtgca agtttctgca
    21061 ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc
    21121 taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt
    21181 tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg
    21241 ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa
    21301 caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga
    21361 tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac
    21421 caaatgccgt gacatccgcg tcgtgataac gtgcttcgtc cgagacaacg atatcgccca
    21481 ctttcaacgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc
    21541 agtgttccaa cagcaaagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca
    21601 gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga
    21661 tagtttgacg tttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa
    21721 tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat
    21781 gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga
    21841 aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag
    21901 atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt
    21961 ctgcaacaaa agacgcaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt
    22021 acccactcga tggaagtcca gcaggtgggg cgctacatcg ccaaagaaat tttaagccgt
    22081 ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg accttttgaa
    22141 agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt
    22201 ggcgaagcag cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc
    22261 cagcctctga ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa
    22321 ccgcttaacg agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca
    22381 caaggcattc gtctggtgca tacattgatg cggatgaatc tcacctgggc acaggttggc
    22441 ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac
    22501 tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt
    22561 aaagaactta atttggcgct ttacagtcgt tttccattaa cttggattat ggaagctgcc
    22621 gacgacatct cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc
    22681 gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc
    22741 ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt
    22801 acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac
    22861 gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca
    22921 ttattggaag atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta
    22981 aaacatgtgt ttagccatcc agatgtcgag cagcttgaat tgcagggcta tcgggtcatt
    23041 agcggattat tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa
    23101 ctggtagaaa aagaacgggt gaaacgtttc cctattgaat cgcgcttatt ccacaaactc
    23161 tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct
    23221 gagtttccgc tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt
    23281 atgaccgacc tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca
    23341 ggcttttgta aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt
    23401 cgcgctataa aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg
    23461 aaatacatga aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg
    23521 ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg
    23581 ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa
    23641 ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat
    23701 gattctccgt tctgccagga aggttcaccg ttccagagct ctccgttctg ccaggggggc
    23761 cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc
    23821 attgatgccg ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg
    23881 attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg
    23941 cgctctgata tcgcgctgat tcagatccag aacccgaaaa acctgaccgc aattaagatg
    24001 gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggc
    24061 ctgggagaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgtc
    24121 gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt
    24181 gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac
    24241 ggcggcaaca tcggtatcgg ttttgctatc ccgagcaaca tggtgaaaaa cctgacctcg
    24301 cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg
    24361 aattccgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag
    24421 gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca
    24481 ctgaacggta agccaatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg
    24541 gtgggcagca aactgaccct gggcttgctg cgcgacggta agcaggtcaa cgtgaatctg
    24601 gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa
    24661 ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaaaacg
    24721 ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg tgattattgg cgcgaaccag
    24781 caggcagtga aaaacatcgc tgaactgcgt aaagttcttg acagcaaacc gtctgtactg
    24841 gcactgaaca ttcagcgcgg cgacagctcc atctacctgt taatgcagta atctccctca
    24901 accccttcct gaaaacggga aggggttctc cttacaatct gtgaacttca ccacaactcc
    24961 atacatcttc atcatccttt aggcatttgc acaatgccgt acgttacgta cttccttatg
    25021 ctaagccgtg cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca
    25081 ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg
    25141 tgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt
    25201 gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtggtac gtcatctgca
    25261 cggtgtgcgg caagggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat
    25321 tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc
    25381 tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gctcaggata gccgtttgcg
    25441 ggaggaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac ttactgaatg
    25501 ggcgcaacgg ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga
    25561 cagcggtcag cttggtgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct
    25621 gactacgccc gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt
    25681 gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga
    25741 acaactgatt acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa
    25801 ctattttacc ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt
    25861 ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc
    25921 tgtgttactc gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc
    25981 gcggctgaaa gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg
    26041 tcacaatgtg caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga
    26101 gtatcggctt aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag
    26161 gttgctgctg tatgtggcgt tacaactgga tgaagagcgg taggttatgc gttaaggttg
    26221 aggcggcgct ggctcattcc cctcagcccg aaggggagag gggaatgccc gaattaaccg
    26281 tcaattcggg attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta
    26341 ttggttacga ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagagcaact
    26401 tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg tagattcggg
    26461 ctgatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc
    26521 tgacgcggat atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa
    26581 tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca
    26641 ggattggtaa tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca
    26701 gtaaattgtg cttattttag catttggcct ggcccgcccg gctcgactgt gatcgcagaa
    26761 agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg
    26821 tggtgataaa attcacgttc tacaccagtt ccagcatcgt tacttccggg cggcagttca
    26881 gacgcaaacc atacaaactg cccacgccac gggttgtgta gatgtgtcgt tcgccaaagg
    26941 catttaatcc ggcgacgtaa cgtttatctt cgacaggggc aaaaggttca ccaaccaacg
    27001 gtacgtgcag ttgcccgccg tgagtatggc cgcacagcat cagatcccag ggttcatcac
    27061 gcatgacttc tttgctgtcg ggattatgcg ccagcaccag tcttggcaga ttggcttcgc
    27121 tggcgggagg cggtttgcat tgtccggccc ataagtcacc ggtgcctacc agttcgaatt
    27181 gcctgttcgg cgtggcgatc accgtagcct ggttaaacaa caccgtgatg cccgctgatt
    27241 tcaacgtctc gccaattaag tgattttttt ctgtaccaac agggcgatcg tggttgccga
    27301 agcaggcaaa cgtcggcgca cattcggcaa ggggggagag gacatcacta aacgccgaaa
    27361 aattcagcgg catatcaaat aatacgtaat cgccgcccag taatatcaaa tcgggctttt
    27421 gttctatgcc aagagcaatc gcgtcagaaa tcaggcttaa aggaacaaaa cgagagtaat
    27481 ggagatcggc cagaaaaaga attttgaatg gtgctgcgtt gtctttaaaa aaggcgaggc
    27541 ggtgacggat taattcaaac cagcctggct cccagtaatg catataaccg aaacctgagc
    27601 tcgtggctat cgtcgcggca gtagcctgca acaatcggcg gcgtgaaatc atcgctcatc
    27661 cctgcaaaaa aaatcgggca gcgtcgtgct gcccgtgtgc atacttttag tcgatggtac
    27721 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27781 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27841 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27901 tctgaccaat gtatacgccc atggaaatga cggaaccttc ttcgacaatc accccttcaa
    27961 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat gatggttggg ttagcctgca
    28021 gcggttccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    28081 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    28141 cgatgttgac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    28201 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    28261 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    28321 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    28381 cgtcaatttt ttccgctaca cgcagtgcac cggaatccag cagggcgatc acctgattta
    28441 ccgcttcgcg ggtaacggtg tctgcgttgg ctggcgtgat ctcggcacgg cgttcaaaag
    28501 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28561 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28621 actcgttatt aagtgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28681 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28741 cgactctcgc cagcagtcct ggctggtcga gcgcgataag ctcaaggaac gatttgcggt
    28801 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28861 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagtacttgc tccagaccaa
    28921 accgaataac ctcatgacga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28981 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    29041 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    29101 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    29161 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    29221 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    29281 tgcgcagtag tgccagtgcc tggagttggt gatgacgaac ccgttcgcgc atatccggcg
    29341 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    29401 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc accgtcaggc
    29461 ataccagata acgaagtcgg ttttctgttt gcacttcttc ggcaaactgc ttgatgactt
    29521 ccgggtcctg aatatcgcgg cgttgggcgg tcaccgacat caacaggtgc tggcgaacca
    29581 gccaggcaac cagctgtgtt tcgcgcgagt taagcccgtg gagttcggca aaatgcacta
    29641 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29701 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29761 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29821 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatacg
    29881 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29941 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttcc ggaatattac
    30001 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    30061 tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    30121 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    30181 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    30241 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    30301 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    30361 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    30421 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    30481 caaacccaac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30541 gagtgtggat atcgcgcaag ccgccagggc tacttttgat gtctggttca agattgtagc
    30601 tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc gcgtagaact
    30661 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30721 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30781 cttccagcat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30841 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30901 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30961 cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    31021 ggagctggtc gataaactcg gtgcgcgcct caatcaactg ttctgcagag atcccattgt
    31081 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccaccgacgg
    31141 ttaattcatc acggggccag acgcatggat tttgcggttg accgggcagg gtggggagag
    31201 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    31261 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    31321 gggatggtgt catccttgcg tagcgtcaga atttcgcagc cgttatcagt caccacaata
    31381 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    31441 atggtgcgga tctctttttt acccgcgttg accattggct cgatggtgaa cgtcatccca
    31501 ggtttcagta cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31561 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31621 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt gaccatgcgc
    31681 agcgccaggt acaggctttc ttgcgtgatg cggcacagac gttcgcccat gatggtcggc
    31741 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31801 tcaatgttaa cgatatcgcc atctttcagc agcttcgcat cgtccgggat accgtggcac
    31861 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31921 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31981 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    32041 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    32101 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    32161 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaacgtttt ctataataga
    32221 aaattcaacg tctgatgctg tacacagcgc caacaattat tggtgtccat gacgtatttg
    32281 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    32341 aatctcactt tgtgtaacaa cacacacgta tcggcacgta ttccggggtg ccctttgggg
    32401 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    32461 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    32521 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32581 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgct
    32641 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32701 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32761 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32821 caggacggta ctttcgaaaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32881 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32941 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cctgggtatt
    33001 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    33061 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    33121 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    33181 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    33241 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    33301 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    33361 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    33421 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    33481 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33541 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33601 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33661 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33721 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33781 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgagaacatc aacattcgcc
    33841 gcgttgctgt gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33901 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33961 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    34021 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    34081 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    34141 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    34201 ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    34261 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    34321 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    34381 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    34441 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    34501 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34561 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34621 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34681 gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34741 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34801 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34861 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34921 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34981 aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga
    35041 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    35101 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    35161 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgttag
    35221 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    35281 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    35341 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    35401 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    35461 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    35521 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35581 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35641 ccgtacactg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35701 tatggcgtcc gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35761 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35821 acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35881 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35941 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    36001 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    36061 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    36121 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    36181 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    36241 gcctggaatt ctctccccgc tatgccgtaa tggacgatgg agcgagtgcg aaacttctta
    36301 aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa caagccgctt
    36361 gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt ggcgctgctg
    36421 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    36481 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36541 tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa cctatccagc
    36601 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36661 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg ccggatcaag
    36721 cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg gctaccatga
    36781 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36841 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36901 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36961 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    37021 catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg gaggcgttcg
    37081 aacaaggtca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    37141 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc gtactggaaa
    37201 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcgc
    37261 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    37321 agtcgcgcta tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag
    37381 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    37441 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    37501 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37561 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37621 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt
    37681 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37741 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37801 gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag aacgtattcg
    37861 taaatctgaa gcgctaacag ccgggaatag cggtctgacg ctgaatattg cggcgaacta
    37921 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37981 aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct gtatgcatga
    38041 actggcccct gtagatttag taattaggac tgggggggag catcgcatta gtaacttttt
    38101 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    38161 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    38221 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    38281 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    38341 gttcgccatc gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    38401 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    38461 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    38521 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38581 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38641 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactacg acgagaatca
    38701 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactctgg
    38761 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38821 taaaacctgg caaggcttta taggtggact agcgaccgca gcggtaatct catggggtta
    38881 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38941 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    39001 taaggacagc agtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    39061 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    39121 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    39181 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    39241 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    39301 gaatatgtta tcgccctgat cccgctgggc ggttatgtca aaatgctgga tgagcgcgca
    39361 gaaccggtcg ttccggaact cctccaccat gccttcaata ataaatctgt cggccaacga
    39421 gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    39481 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggtgaaat agcagccaat
    39541 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39601 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39661 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39721 cgtcactggg cgtttgagcc ggataaagaa gatccggtat cttcgctggg gattcgtcct
    39781 cgtgggccgc aaattgaacc tgtactggaa aatgtgcaac caaactcggc ggcaagcaag
    39841 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39901 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39961 aggcagggaa gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    40021 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    40081 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    40141 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    40201 ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    40261 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    40321 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    40381 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    40441 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    40501 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40561 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40621 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg gttaatgatg
    40681 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40741 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40801 ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt
    40861 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40921 tggaagactt ctactacagc gtcggtaaat atagcgccag cgtaaaagct gtcgtgaccc
    40981 cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa
    41041 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    41101 tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa taccagaaac
    41161 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggatcgc ggttatgccc
    41221 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    41281 cggtgaacat taccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    41341 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    41401 acggcaccaa agtgaccaag atggaagatg acatcaaaaa gcttctcggt cgctatggtt
    41461 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    41521 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41581 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggc
    41641 tgggaagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41701 ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaggtagat gtcgtctaca
    41761 aggtaaaaga gcgcaacacc ggtagcttca acttcgggat tggttacggt actgaaagtg
    41821 gcgtgagctt ccaggctggt gtgcagcagg ataactggtt aggtacaggt tatgctgttg
    41881 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41941 acttcactgt agatggtgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    42001 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    42061 tcccgatcaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    42121 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    42181 gcacctctga tcaggataat agcttcaaaa cggacgactt cacgttcaac tatggttgga
    42241 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    42301 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    42361 cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc cgctggggtt
    42421 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    42481 ccagcaccgt gcgtggcttc cagtccaata caattggtcc gaaagcagtt tacttcccgc
    42541 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42601 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42661 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcct
    42721 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42781 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42841 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42901 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42961 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    43021 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    43081 ttagcggcag gtctcggttt agcactggca acttctgctc aggcggctga caaaattgca
    43141 atcgtcaaca tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    43201 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    43261 gctaaaatga aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa gctggaaaaa
    43321 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    43381 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    43441 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac
    43501 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43561 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43621 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43681 gttaacccaa aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc
    43741 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43801 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43861 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43921 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43981 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    44041 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    44101 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    44161 attggcgatc gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    44221 actattattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    44281 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    44341 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    44401 gtgacggtta cgggcatgag tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    44461 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    44521 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44581 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44641 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44701 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44761 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44821 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44881 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44941 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    45001 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    45061 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    45121 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    45181 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    45241 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    45301 atactaaaat tggccgcgat aatgagattt atcagttcgc ctccatcggc gaagttaacc
    45361 aggatctgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    45421 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    45481 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45541 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45601 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcacgtg atggttggcg
    45661 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45721 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45781 ccgctatccg caatgcgtat aagctgattt atcgtagcgg taaaacgctc gatgaagtga
    45841 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45901 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat taacgattgc
    45961 cctggtcgcc ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    46021 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    46081 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    46141 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    46201 gccagatatt tttgttggta ttgatgcgcc tgacttcaat attactcttg aaggtaacct
    46261 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    46321 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    46381 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    46441 tgccatgccg ttagatccag ataaaaatgc cgcccgtgat gtgctgggga tccctcacga
    46501 tgcccactgc ctggcgttgc taccagggag ccgtggtgca gaagttgaaa tgcttagtgc
    46561 cgatttcctg aaaacggccc agcttttgcg ccagacatat tcggatctcg aaatcgtggt
    46621 gccactggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46681 agacctttca gttcatttgc tggatgggat gggccgtgag gcgatggtcg ccagcgatgc
    46741 ggcgctactg gcgtcgggta cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46801 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46861 ttatgtctcg ctgccaaatc tgctggcggg tagagagtta gtcaaagaat tattgcagga
    46921 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46981 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    47041 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    47101 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    47161 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    47221 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    47281 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    47341 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    47401 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg cgatagccgc
    47461 gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg
    47521 gcgctggata ttgttttccc gcaatatggt tttgcccaac acaaagggta cccaaccgct
    47581 tttcatctgg aaaaactggc tgaacacggc gcgaccgaac accatcggcg cagctttggg
    47641 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47701 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47761 atcgatggcc tggccaaaac cgcaccgttg gtaaaaaagg cggcggcgtt gggtatgcca
    47821 gcactggcga tcaccgattt caccaacctt tgtggtctgg tgaagttcta cggagcggga
    47881 catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg cgacctgctg
    47941 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    48001 acgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    48061 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg acgcatgggc
    48121 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    48181 tatgaagaac acttcccgga tcgctatttt ctcgagctga tccgcactgg caggccggat
    48241 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    48301 gcgaccaacg acgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    48361 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    48421 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc cgaagccctt
    48481 gccaacaccg ttgagatcgc caaacgctgt aacgtaaccg tgcgtcttgg tgaatacttc
    48541 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48601 gagggcctgg aagagcgtct ggccttttta ttccctgacg aggaagaacg tgttaagcgc
    48661 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc
    48721 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48781 gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaattacc
    48841 gacctcgacc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48901 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgagcac
    48961 gtagcggaca tgtacggtcg tgatgcggta tcgcagatca tcaccttcgg tacaatggcg
    49021 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgat
    49081 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    49141 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    49201 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    49261 gcgccgacca aaattaccga ttttgcgccg ctttactgcg atgaagaggg caaacatccg
    49321 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    49381 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    49441 aagaatggcg agccgccgct ggatatcgct gcgatcccgc tggatgataa gaaaagcttc
    49501 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49561 gacctgatca agcgtctaca acctgactgc ttcgaagata tgatcgccct agtggcactg
    49621 ttccgccccg gtccgttgca atcagggatg gtggataact ttatcgaccg taaacatggt
    49681 cgtgaagaga tctcctatcc ggacgtacag tggcagcatg aaagcctgaa accggtactg
    49741 gagccaacct acggcattat cctgtatcag gaacaggtca tgcagattgc gcaggtgctt
    49801 tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa gaaaaagccg
    49861 gaagagatgg ctaagcaacg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49921 gctgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgctggtta cggatttaac
    49981 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcacac
    50041 tatcctgcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    50101 gtgggtctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    50161 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg
    50221 atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    50281 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt gaaccgtcgc
    50341 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    50401 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    50461 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    50521 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50581 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50641 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg
    50701 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50761 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50821 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50881 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50941 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    51001 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    51061 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    51121 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    51181 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    51241 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    51301 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    51361 aaaatcttcg ccgatcttgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt
    51421 ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    51481 cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtcttga cggtcgtccg
    51541 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51601 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51661 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatccggg cgtgggcgca
    51721 gaagaacgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgcctcggt
    51781 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggcgcgct ggcgattggc
    51841 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51901 tgtgcgtcca ttctgtggaa gagcgctgac aaagcgccgc tggcagctga agcgatgggt
    51961 atcatcgctc cgcgtctgaa agaactaaaa ctgatcgact ccatcatccc ggaaccgctg
    52021 ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    52081 gatctggccg atctcgacgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    52141 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    52201 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    52261 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    52321 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    52381 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    52441 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    52501 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52561 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52621 acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg tttacctacg
    52681 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52741 aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc aaggctgatg
    52801 tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag
    52861 cagaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggaa
    52921 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52981 tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag
    53041 tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg cgccgtgaat
    53101 ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa tggccggttc
    53161 atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac tggatcaaac
    53221 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac acccattttc
    53281 atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct
    53341 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca
    53401 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    53461 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    53521 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53581 agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53641 atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt aacgatgcgg
    53701 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53761 aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa ttcctcgacg
    53821 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53881 gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aaacgctctt
    53941 acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa gatcccgatt
    54001 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    54061 acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    54121 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    54181 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    54241 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    54301 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    54361 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    54421 ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    54481 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagttttct actgcgatat
    54541 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54601 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54661 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54721 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54781 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54841 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54901 aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc
    54961 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    55021 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    55081 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    55141 tggtcgaacg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag gcgcggcagg
    55201 cgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac
    55261 atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc ggccctgccg
    55321 ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc
    55381 tcgcccgcac gcggggagaa ctggagcagt gggcattggc gcatggttta cgctggattg
    55441 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    55501 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccgcacttt
    55561 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55621 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgccgcgcgg
    55681 cgattatccg tcgctggctg gcagggcaga atgcaccaat gccttcccgc gacgcgctgg
    55741 tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt ttacgcttgg
    55801 gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55861 aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55921 tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55981 tcagcgtgcg tttcaaagcg gcaggattgc tgcatattgt cgggcgcaac ggcggacgta
    56041 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    56101 tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    56161 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    56221 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc gctcaccacc
    56281 accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag ctcacgggtt
    56341 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcgctggct
    56401 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    56461 gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac
    56521 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56581 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56641 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56701 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56761 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56821 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56881 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56941 ctctatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    57001 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    57061 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    57121 gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc cgcagaagtg
    57181 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    57241 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    57301 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg cgtgatctgg
    57361 ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca accttcatga
    57421 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    57481 cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat ctgcgttttg
    57541 acattcgggc gtccagcctg tcagagtatt acaaagagcg tctactcgcc gccagccatc
    57601 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57661 tgaaccgcga agccgcgcta gcccggctgg tggcagtgat taaagattta acaacagaac
    57721 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57781 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57841 aagaaggaat agatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57901 actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc acaggctgcc
    57961 gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga ttgcgaagga
    58021 atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    58081 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    58141 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    58201 ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta tacgctggaa
    58261 ccggcacaat ctagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    58321 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    58381 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    58441 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    58501 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58561 taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcagtat
    58621 ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga taaggcgttt
    58681 acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag cccaacaaag
    58741 cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa ctgcgtttct
    58801 ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag
    58861 cccgattgcg ccagtgtaag tggatgcccg gctttggcaa aggcatccgt gacaaattcc
    58921 gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc tttcgccagg
    58981 ccgctgacgc actttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
    59041 tcaccataaa gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt
    59101 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    59161 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    59221 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    59281 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    59341 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    59401 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    59461 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga aacaggcggc
    59521 agtacgcctt tggtttatcc attttataca atccatgtaa aaaaagggcc ctgaaattca
    59581 ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgatga tgtcaccagt
    59641 cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag
    59701 gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat
    59761 cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc
    59821 gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag
    59881 aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgcctcgtt cgtcgtagtt
    59941 ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag
    60001 gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt
    60061 acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc
    60121 gtccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac
    60181 atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg atgttagcac cagcagcgaa
    60241 atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc
    60301 cagtgaaccc ggaccggctt taaccacggc acgaatttct tcttcggtcg cgaaagtcag
    60361 cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg
    60421 caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca gcagagtctt
    60481 aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt
    60541 cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat
    60601 agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc
    60661 ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt
    60721 gtcggcttgt acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc
    60781 atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc
    60841 tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa
    60901 cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg
    60961 aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg
    61021 gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa
    61081 atcagctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc
    61141 acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa
    61201 cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc
    61261 ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt
    61321 acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaaagtgatt
    61381 tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag
    61441 cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca
    61501 gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgtg ggtcctgcgc
    61561 cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg
    61621 cttttcgacc tctgcggtaa aactcaccgc catctctgcc gctggcgcac tttgcgcata
    61681 gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac
    61741 cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca
    61801 aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgga aagtagagcg
    61861 tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg
    61921 ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaacgcttc
    61981 caggccgcga acggcgtcgg cctggttgta gggagcaatg agatgcagtt cgccgttggc
    62041 gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat
    62101 aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg
    62161 atacatacag cctgacgata gcagcctggc gtaccgctgg tgacttcgca gctatgcagt
    62221 aataccgcat tggccttcat tttcgaggcg ttgatttgca tccgcttacg cgcggttgga
    62281 atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga
    62341 tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg
    62401 cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt
    62461 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
    62521 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
    62581 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
    62641 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
    62701 taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
    62761 tcttcacgcg ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg
    62821 tctttcgctg gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg
    62881 atttgcgcgt cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac aattttcaga
    62941 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
    63001 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt
    63061 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
    63121 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
    63181 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
    63241 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
    63301 ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg
    63361 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
    63421 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
    63481 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
    63541 cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
    63601 gtcgcgttat agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
    63661 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
    63721 accagacccg gcaatgcttc cggtaacagc actttacgga cgatctgcat cggcgtggca
    63781 cccattgcac gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
    63841 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caatcgctgc ctgcaaacca
    63901 atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
    63961 gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
    64021 ataatttgcc ccgggcgcgt aacataaagc aggacgccga ccggcagacc aatcacaaag
    64081 ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcaac
    64141 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
    64201 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
    64261 cttaacgcca ccggcgtaat ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
    64321 acgcgcggtt tcagaaagca gcggggcatc gaccgattga ccggtaaact ccaggcgcaa
    64381 catcggcacg cagtcagtaa atggctccgc ttgcagacgt tcctggtaat cttccgggat
    64441 atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa
    64501 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc
    64561 acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
    64621 gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct
    64681 ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg caatcgccac
    64741 acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc
    64801 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag
    64861 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
    64921 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag
    64981 ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg
    65041 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
    65101 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
    65161 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca
    65221 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
    65281 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
    65341 cgtcccatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct
    65401 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
    65461 atttatcgac ggtgttattg acaccatgcg cgagctaaaa aaaatgggct ttgcgctggt
    65521 ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
    65581 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
    65641 ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgattgccg
    65701 caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
    65761 ttcttatatg gtgggagata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg
    65821 aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga
    65881 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
    65941 gcaatgatta aaagatgagc ggttgaaata aaaatgcatt tttccgcttg tcttcctgag
    66001 ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag
    66061 ccggttcggt tgaagag
//
LOCUS       DAZUFB010000013        65939 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZUFB010000013 DAZUFB010000000
VERSION     DAZUFB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.013"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /locus_tag="ACHY3M_000987"
     CDS             complement(<1..153)
                     /locus_tag="ACHY3M_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315946.1"
                     /translation="MNMLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKR
                     ADSRFEL"
     gene            complement(325..1236)
                     /gene="gsiD"
                     /locus_tag="ACHY3M_000988"
     CDS             complement(325..1236)
                     /gene="gsiD"
                     /locus_tag="ACHY3M_000988"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFS4315947.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQSPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1239..2159)
                     /gene="gsiC"
                     /locus_tag="ACHY3M_000989"
     CDS             complement(1239..2159)
                     /gene="gsiC"
                     /locus_tag="ACHY3M_000989"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFS4315948.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETRV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2177..3715)
                     /gene="gsiB"
                     /locus_tag="ACHY3M_000990"
     CDS             complement(2177..3715)
                     /gene="gsiB"
                     /locus_tag="ACHY3M_000990"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFS4315949.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3735..5606)
                     /gene="gsiA"
                     /locus_tag="ACHY3M_000991"
     CDS             complement(3735..5606)
                     /gene="gsiA"
                     /locus_tag="ACHY3M_000991"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFS4315950.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5593..6558)
                     /gene="iaaA"
                     /locus_tag="ACHY3M_000992"
     CDS             complement(5593..6558)
                     /gene="iaaA"
                     /locus_tag="ACHY3M_000992"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4315951.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6762..7997
                     /gene="moeA"
                     /locus_tag="ACHY3M_000993"
     CDS             6762..7997
                     /gene="moeA"
                     /locus_tag="ACHY3M_000993"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFS4315952.1"
                     /translation="MEFTTGLMSLDTALNEILSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7997..8746
                     /gene="moeB"
                     /locus_tag="ACHY3M_000994"
     CDS             7997..8746
                     /gene="moeB"
                     /locus_tag="ACHY3M_000994"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFS4315953.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8822..9484)
                     /gene="fsa"
                     /locus_tag="ACHY3M_000995"
     CDS             complement(8822..9484)
                     /gene="fsa"
                     /locus_tag="ACHY3M_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4315954.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9615..10514
                     /gene="ybiY"
                     /locus_tag="ACHY3M_000996"
     CDS             9615..10514
                     /gene="ybiY"
                     /locus_tag="ACHY3M_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFS4315955.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10520..12952
                     /gene="ybiW"
                     /locus_tag="ACHY3M_000997"
     CDS             10520..12952
                     /gene="ybiW"
                     /locus_tag="ACHY3M_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFS4315956.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13098..13913
                     /gene="ybiV"
                     /locus_tag="ACHY3M_000998"
     CDS             13098..13913
                     /gene="ybiV"
                     /locus_tag="ACHY3M_000998"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFS4315957.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14065..15330
                     /gene="ybiU"
                     /locus_tag="ACHY3M_000999"
     CDS             14065..15330
                     /gene="ybiU"
                     /locus_tag="ACHY3M_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFS4315958.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15567..17159)
                     /gene="ybiT"
                     /locus_tag="ACHY3M_001000"
     CDS             complement(15567..17159)
                     /gene="ybiT"
                     /locus_tag="ACHY3M_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFS4315959.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17378..18298
                     /gene="ldtB"
                     /locus_tag="ACHY3M_001001"
     CDS             17378..18298
                     /gene="ldtB"
                     /locus_tag="ACHY3M_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4315960.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKN
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18357..19475)
                     /gene="ybiR"
                     /locus_tag="ACHY3M_001002"
     CDS             complement(18357..19475)
                     /gene="ybiR"
                     /locus_tag="ACHY3M_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFS4315961.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19472..19939)
                     /gene="mntR"
                     /locus_tag="ACHY3M_001003"
     CDS             complement(19472..19939)
                     /gene="mntR"
                     /locus_tag="ACHY3M_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFS4315962.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20125..20253
                     /gene="mntS"
                     /locus_tag="ACHY3M_001004"
     CDS             20125..20253
                     /gene="mntS"
                     /locus_tag="ACHY3M_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFS4315963.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20525..22108
                     /gene="opgE"
                     /locus_tag="ACHY3M_001005"
     CDS             20525..22108
                     /gene="opgE"
                     /locus_tag="ACHY3M_001005"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS4315964.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22157..22672)
                     /gene="ompX"
                     /locus_tag="ACHY3M_001006"
     CDS             complement(22157..22672)
                     /gene="ompX"
                     /locus_tag="ACHY3M_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFS4315965.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22725..22790)
                     /locus_tag="ACHY3M_001007"
     CDS             complement(22725..22790)
                     /locus_tag="ACHY3M_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4315966.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23025..23912
                     /gene="rhtA"
                     /locus_tag="ACHY3M_001008"
     CDS             23025..23912
                     /gene="rhtA"
                     /locus_tag="ACHY3M_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFS4315967.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24211..24714
                     /gene="dps"
                     /locus_tag="ACHY3M_001009"
                     /gene_synonym="pexB"
     CDS             24211..24714
                     /gene="dps"
                     /locus_tag="ACHY3M_001009"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFS4315968.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25118..25864
                     /gene="glnH"
                     /locus_tag="ACHY3M_001010"
     CDS             25118..25864
                     /gene="glnH"
                     /locus_tag="ACHY3M_001010"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFS4315969.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26003..26662
                     /gene="glnP"
                     /locus_tag="ACHY3M_001011"
     CDS             26003..26662
                     /gene="glnP"
                     /locus_tag="ACHY3M_001011"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFS4315970.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            26659..27381
                     /gene="glnQ"
                     /locus_tag="ACHY3M_001012"
     CDS             26659..27381
                     /gene="glnQ"
                     /locus_tag="ACHY3M_001012"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFS4315971.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            27498..29723
                     /gene="ybiO"
                     /locus_tag="ACHY3M_001013"
     CDS             27498..29723
                     /gene="ybiO"
                     /locus_tag="ACHY3M_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFS4315972.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(29720..30646)
                     /gene="rlmF"
                     /locus_tag="ACHY3M_001014"
     CDS             complement(29720..30646)
                     /gene="rlmF"
                     /locus_tag="ACHY3M_001014"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFS4315973.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDELNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            30922..31182
                     /gene="mcbA"
                     /locus_tag="ACHY3M_001015"
     CDS             30922..31182
                     /gene="mcbA"
                     /locus_tag="ACHY3M_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFS4315974.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            31446..33728
                     /gene="fiu"
                     /locus_tag="ACHY3M_001016"
     CDS             31446..33728
                     /gene="fiu"
                     /locus_tag="ACHY3M_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFS4315975.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGVNANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSVNRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            33770..34447
                     /gene="ybiX"
                     /locus_tag="ACHY3M_001017"
     CDS             33770..34447
                     /gene="ybiX"
                     /locus_tag="ACHY3M_001017"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFS4315976.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            34521..34787
                     /gene="ybiI"
                     /locus_tag="ACHY3M_001018"
     CDS             34521..34787
                     /gene="ybiI"
                     /locus_tag="ACHY3M_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFS4315977.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            35052..35312
                     /gene="ybiJ"
                     /locus_tag="ACHY3M_001019"
     CDS             35052..35312
                     /gene="ybiJ"
                     /locus_tag="ACHY3M_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFS4315978.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35416..35475)
                     /gene="ybiE"
                     /locus_tag="ACHY3M_001020"
     CDS             complement(35416..35475)
                     /gene="ybiE"
                     /locus_tag="ACHY3M_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFS4315979.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(35542..36627)
                     /gene="hcxB"
                     /locus_tag="ACHY3M_001021"
     CDS             complement(35542..36627)
                     /gene="hcxB"
                     /locus_tag="ACHY3M_001021"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFS4315980.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(36768..37730)
                     /gene="ybiB"
                     /locus_tag="ACHY3M_001022"
     CDS             complement(36768..37730)
                     /gene="ybiB"
                     /locus_tag="ACHY3M_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFS4315981.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(37758..39908)
                     /gene="dinG"
                     /locus_tag="ACHY3M_001023"
     CDS             complement(37758..39908)
                     /gene="dinG"
                     /locus_tag="ACHY3M_001023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFS4315982.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTSIAIDDDLWRRLSTDKASCLNRNCYYYRECTFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            40028..40510
                     /gene="ybiA"
                     /locus_tag="ACHY3M_001024"
     CDS             40028..40510
                     /gene="ybiA"
                     /locus_tag="ACHY3M_001024"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFS4315983.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(40743..42107)
                     /gene="rhlE"
                     /locus_tag="ACHY3M_001025"
     CDS             complement(40743..42107)
                     /gene="rhlE"
                     /locus_tag="ACHY3M_001025"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFS4315984.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDICVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            42336..43007
                     /gene="cecR"
                     /locus_tag="ACHY3M_001026"
     CDS             42336..43007
                     /gene="cecR"
                     /locus_tag="ACHY3M_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFS4315985.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            43010..44005
                     /gene="hlyD"
                     /locus_tag="ACHY3M_001027"
     CDS             43010..44005
                     /gene="hlyD"
                     /locus_tag="ACHY3M_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFS4315986.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            43998..45734
                     /gene="ybhF"
                     /locus_tag="ACHY3M_001028"
     CDS             43998..45734
                     /gene="ybhF"
                     /locus_tag="ACHY3M_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4315987.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            45727..46860
                     /gene="ybhS"
                     /locus_tag="ACHY3M_001029"
     CDS             45727..46860
                     /gene="ybhS"
                     /locus_tag="ACHY3M_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315988.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            46871..47977
                     /gene="ybhR"
                     /locus_tag="ACHY3M_001030"
     CDS             46871..47977
                     /gene="ybhR"
                     /locus_tag="ACHY3M_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4315989.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(47939..48349)
                     /gene="ybhQ"
                     /locus_tag="ACHY3M_001031"
     CDS             complement(47939..48349)
                     /gene="ybhQ"
                     /locus_tag="ACHY3M_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFS4315990.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            48482..49243
                     /gene="ybhP"
                     /locus_tag="ACHY3M_001032"
     CDS             48482..49243
                     /gene="ybhP"
                     /locus_tag="ACHY3M_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4315991.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            49240..50481
                     /gene="clsB"
                     /locus_tag="ACHY3M_001033"
     CDS             49240..50481
                     /gene="clsB"
                     /locus_tag="ACHY3M_001033"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFS4315992.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            50481..51437
                     /gene="ybhN"
                     /locus_tag="ACHY3M_001034"
     CDS             50481..51437
                     /gene="ybhN"
                     /locus_tag="ACHY3M_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFS4315993.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(51473..52186)
                     /gene="ybhM"
                     /locus_tag="ACHY3M_001035"
     CDS             complement(51473..52186)
                     /gene="ybhM"
                     /locus_tag="ACHY3M_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4315994.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(52391..53095)
                     /gene="ybhL"
                     /locus_tag="ACHY3M_001036"
     CDS             complement(52391..53095)
                     /gene="ybhL"
                     /locus_tag="ACHY3M_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4315995.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(53232..53684)
                     /gene="moaE"
                     /locus_tag="ACHY3M_001037"
     CDS             complement(53232..53684)
                     /gene="moaE"
                     /locus_tag="ACHY3M_001037"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFS4315996.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(53686..53931)
                     /gene="moaD"
                     /locus_tag="ACHY3M_001038"
     CDS             complement(53686..53931)
                     /gene="moaD"
                     /locus_tag="ACHY3M_001038"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFS4315997.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(53924..54409)
                     /gene="moaC"
                     /locus_tag="ACHY3M_001039"
     CDS             complement(53924..54409)
                     /gene="moaC"
                     /locus_tag="ACHY3M_001039"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFS4315998.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(54412..54924)
                     /gene="moaB"
                     /locus_tag="ACHY3M_001040"
     CDS             complement(54412..54924)
                     /gene="moaB"
                     /locus_tag="ACHY3M_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFS4315999.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(54946..55935)
                     /gene="moaA"
                     /locus_tag="ACHY3M_001041"
     CDS             complement(54946..55935)
                     /gene="moaA"
                     /locus_tag="ACHY3M_001041"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFS4316000.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(55923..56067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            56332..57240
                     /gene="yvcK"
                     /locus_tag="ACHY3M_001042"
     CDS             56332..57240
                     /gene="yvcK"
                     /locus_tag="ACHY3M_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4316001.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(57432..59453)
                     /gene="uvrB"
                     /locus_tag="ACHY3M_001043"
     CDS             complement(57432..59453)
                     /gene="uvrB"
                     /locus_tag="ACHY3M_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4316002.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(60032..60709)
                     /gene="bioD"
                     /locus_tag="ACHY3M_001044"
     CDS             complement(60032..60709)
                     /gene="bioD"
                     /locus_tag="ACHY3M_001044"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS4316003.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(60702..61457)
                     /gene="bioC"
                     /locus_tag="ACHY3M_001045"
     CDS             complement(60702..61457)
                     /gene="bioC"
                     /locus_tag="ACHY3M_001045"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFS4316004.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(61444..62598)
                     /gene="bioF"
                     /locus_tag="ACHY3M_001046"
     CDS             complement(61444..62598)
                     /gene="bioF"
                     /locus_tag="ACHY3M_001046"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFS4316005.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(62595..63635)
                     /gene="bioB"
                     /locus_tag="ACHY3M_001047"
     CDS             complement(62595..63635)
                     /gene="bioB"
                     /locus_tag="ACHY3M_001047"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFS4316006.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            63722..65011
                     /gene="bioA"
                     /locus_tag="ACHY3M_001048"
     CDS             63722..65011
                     /gene="bioA"
                     /locus_tag="ACHY3M_001048"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFS4316007.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            65070..65546
                     /gene="ybhB"
                     /locus_tag="ACHY3M_001049"
     CDS             65070..65546
                     /gene="ybhB"
                     /locus_tag="ACHY3M_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFS4316008.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT        15774 a        17732 c        16776 g        15657 t
ORIGIN      
        1 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
       61 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      121 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg
      181 ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac
      241 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc
      301 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
      361 accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac
      421 agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc
      481 tggtgtcggc gactgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
      541 ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg
      601 acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
      661 ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat
      721 ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
      781 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca accgatccca
      841 ccagccttca taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa
      901 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat
      961 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
     1021 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
     1081 cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
     1141 tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt
     1201 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
     1261 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
     1321 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
     1381 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag
     1441 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg
     1501 aggacaaccc tggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
     1561 aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg
     1621 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
     1681 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
     1741 atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg
     1801 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
     1861 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
     1921 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
     1981 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgggc
     2041 ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc
     2101 gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt
     2161 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
     2221 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
     2281 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
     2341 ccgccggatc attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt
     2401 aaaatgcggt attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg
     2461 cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
     2521 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct
     2581 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
     2641 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
     2701 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
     2761 cgatacttgg cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca
     2821 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg
     2881 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
     2941 cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact
     3001 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
     3061 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
     3121 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
     3181 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
     3241 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
     3301 cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg
     3361 gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct
     3421 ggaatttaat tccttcccgc aatttcacga tgtaagtaat gccgtcatcg gaaacggtat
     3481 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
     3541 aaaacgattt cgctacggcc tgagataacg tgtcatttgc gtcatacgga tcgagcgtgg
     3601 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
     3661 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
     3721 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
     3781 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
     3841 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
     3901 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
     3961 cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
     4021 cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
     4081 agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct
     4141 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
     4201 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
     4261 cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca
     4321 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
     4381 tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta
     4441 tcgattctct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
     4501 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
     4561 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
     4621 aaaccgctgc gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca
     4681 tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc
     4741 aacgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg aacagcagct
     4801 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt
     4861 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
     4921 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
     4981 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc
     5041 accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
     5101 agttgatgcg gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc
     5161 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
     5221 tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat
     5281 atcatcgcca tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt
     5341 tcaatcactt cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc
     5401 gcctgttcca acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg
     5461 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
     5521 attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg
     5581 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
     5641 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
     5701 gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
     5761 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
     5821 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccgg tacaagaaac
     5881 cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg
     5941 tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa
     6001 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
     6061 atggtcgagg acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg
     6121 cgtggagaaa atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc
     6181 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
     6241 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
     6301 cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt
     6361 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc
     6421 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg
     6481 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
     6541 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
     6601 tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
     6661 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
     6721 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
     6781 tgatgtcgct cgacaccgcg cttaatgaga tactttctcg cgtcacccca ctgaccgccc
     6841 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
     6901 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
     6961 atattgcctc caggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc
     7021 atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag
     7081 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
     7141 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
     7201 cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac
     7261 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
     7321 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc
     7381 tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc
     7441 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
     7501 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
     7561 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
     7621 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     7681 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     7741 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     7801 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     7861 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     7921 aacgcgatcg cggcaatgtg gaagtgggcg aatgggtgga agtagaaccg tttaacgcgt
     7981 tgttcggagg cctgtaatga cggaactcag cgatcaggag atgctgcgct acaaccggca
     8041 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     8101 ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa gcgccggtgt
     8161 cggcaacctg acgctgctcg acttcgactc ggtttcgctc tcgaatctgc aacgccagac
     8221 gctgcacagt gatgctacgg tcgggcaacc gaaggtagaa tccgcccgtg acgccatgac
     8281 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgcttgatg acgctgaact
     8341 tgctgcgatg attgcaaaac acgatctggt actcgactgt acggataacg ttgcggtgcg
     8401 taatcaactg aacgctggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     8461 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     8521 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     8581 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     8641 tggcaaaccg gccagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     8701 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     8761 ccagataaga cgcggcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
     8821 gttaaatcga cgttctgcca aacgctccca gccagtcctg ttcaaacttc gccacagcgg
     8881 cttccaccgc cggatagcta atcatctgtt gtgccacatc cagtggcagc gtaattgatt
     8941 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc gctgccagca
     9001 ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatgc
     9061 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
     9121 gcgccgacag cagcccttgt gctgcgccat ataccgcggt tcccagcgtt ggaatccctt
     9181 ccgcttttaa catcttaata gctgccagcc cctcggcggt caccggaact ttcaccacga
     9241 tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca gcagtggtag
     9301 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca
     9361 caacatccag cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca
     9421 gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt
     9481 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
     9541 tgaacagttg acgcacatca atctaacttt cattcgaaag cattttaatg tttgaacgaa
     9601 acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta
     9661 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
     9721 aaagccgcgc ccgcacgcag gatctgctgt atgacgcacg actgtgtctg gaaggctgcg
     9781 agctgtgcgc taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc
     9841 atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgt ccgacacagg
     9901 cattaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg
     9961 ataaaccgtt ttatgatcgc agcggcggcg gtttaacgct ttcgggtggt gagcccttta
    10021 tgcagccgga aatggcgatg gcgctactgc aagccagcca cgaggcaggc attcatactg
    10081 cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
    10141 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
    10201 gtaacgccgc cagagtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
    10261 ttatccgcgt gccgctgatt cagggcttta atgccgacga aacctctgta aaagccatta
    10321 ccgattttgc cgccgacgag ctgcacgttg gcgaaattca ttttctgccc taccacacgc
    10381 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgctccggaa aaaccgcttg
    10441 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
    10501 ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa
    10561 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca
    10621 gcactatacc gagatgtatc aacaacatct cgataagccg atcccggtac gtcgcgcgct
    10681 ggcactggcg catcacctgg cgaatcgcac catctggatc aaacacgatg agttgatcat
    10741 tggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg
    10801 gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga
    10861 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga
    10921 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa
    10981 agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct
    11041 ggaaaaaggt cttgatggtc tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct
    11101 gacggtgctg gaagatttac atggcgaaca attcctgaaa gcgattgata tcgtgctggt
    11161 ggcagtcagt gaacacattg aacgtttcgc tgccctggcg cgtgaaatgg ctgctaccga
    11221 aactcgcgaa agccgtcgcg atgaactgct gacgatggca gaaaactgcg atcttatcgc
    11281 ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgttacttca tccaattgat
    11341 tttgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc agtatctcta
    11401 cccgtactat cgccgcgacg ttgaactcaa ccagacgctg gatcgcgaac acgccatcga
    11461 gatgctgcat agctgctggc tgaaactgct ggaagtgaac aagatccgct ccggctcaca
    11521 ctcaaaagcc tctgcgggaa gtccgctgta tcagaacgtt accattggcg gacaaaatct
    11581 ggttgatggt caaccaatgg atgcagtgaa tcccctctct tacgcgatcc tcgaatcctg
    11641 cggtcgcctg cgttccactc agcctaacct cagcgtgcgt taccatgcag gaatgagtaa
    11701 tgatttcctc gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa
    11761 caacgacgaa atcgtgatcc cggaatttat taaactcggt attgaaccgc aggacgctta
    11821 cgactacgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg
    11881 taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg
    11941 tgatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt
    12001 taacaatttc gatgaagtaa tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa
    12061 atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct
    12121 ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc
    12181 gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca acagcctggc
    12241 ggcagtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc tggctgccgc
    12301 actggcggat gacttcgatg gcctgactca tgagcaactg cgtcagcggc tgattaacgg
    12361 tgcgccgaag tacggcaacg acgatgatac tgtcgatacg ctgctggctc gcgcttatca
    12421 gacctatatc gacgaactga aacagtacca taatccgcgc tacggtcgtg gtccggttgg
    12481 cggcaactat tacgcgggta cgtcatctat ctccgctaac gtaccgtttg gcgcgcaaac
    12541 tatggcaacg ccggacgggc gtaaagccca caccccgctg gcagaaggcg ctagtccggc
    12601 ctctggtact gaccatctcg gccctactgc ggtcattggc tcggtgggta aactgcctac
    12661 ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccggcaa cgctggagaa
    12721 cgaatctgac aagcagaagc tgatgatcct gctgcgcacc ttcttcgagg tgcataaagg
    12781 ctggcatatt cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc
    12841 ggatcagtac cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct
    12901 ctctccagac gctcaggacg atatcatcgc tcgtactgaa catatgctgt aatctcttcg
    12961 gcccggctgc ggcgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca
    13021 ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc
    13081 gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac ggtacttttc
    13141 ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa
    13201 agcgcggcat tgagttcgtt gtcgccagcg gtaaccagta ttaccagctt atttcgttct
    13261 ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac
    13321 atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg
    13381 agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca
    13441 gcgaaaatgc ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaag
    13501 ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccag
    13561 atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctcgatggc attatgaaac
    13621 ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg
    13681 gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg
    13741 acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg
    13801 ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg
    13861 cgctgaatgt gattcaggcg gtgctggata acacatcccc ttttaacagc tgacctctct
    13921 accggagccg tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca
    13981 ttaaacttgc attaacttat ttataactta tattgtcaca tgcaaatcag aatggtttcg
    14041 aatgaaagtt aatgagaggt ggttatggct tctactttta ccagcgacac attgcctgcc
    14101 gatcacaaag cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc
    14161 cagcagatct ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca
    14221 ctcaaagcac agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt
    14281 catgttactg cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc
    14341 catttccccc gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctggaccgc
    14401 aaccgctttg acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct
    14461 tcacgtcccg agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt
    14521 gaagaaatgg cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga
    14581 aagcaatggt ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccgtccg
    14641 cccggaacga cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg
    14701 ctgcttccag cgtatcagcg cgttttcgcc aacgtcttta atggcaatct ggcgcaatat
    14761 gatccctggc atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc
    14821 aaatgttccg tgtttcggac attccagggc tggacagcgc tctctgatat gctgcctggt
    14881 caggggctgc tgcacgttgt gcccattcct gaagccatgg cgtacgtact gttacgtccg
    14941 ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg
    15001 gtatcagagc aatggcatcc actgttaatt gaggcgttaa ccagcattcc aaaactcgag
    15061 gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat
    15121 caacagggtt ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt
    15181 gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg
    15241 cgcgaggact atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac
    15301 ggtaagcgag cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt
    15361 aagaaaatcg attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg
    15421 cgctacgctt atctggccta caaagtcctg cagattcaat atattacagg tactgcgtag
    15481 atcctgataa gcgtagcgca tcaggctatt ttgcatttgt catcagccgc cgtcaccccg
    15541 acagtatcgg ggtgacggat aatcacttac tcgatccctt tactacgcag gtaatcttcg
    15601 taattaccgc taaagtcgat cacgcgttcc ggggtgattt ccagaatgcg ggtcgccagg
    15661 gagcttacga actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc
    15721 gccatgttca gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc
    15781 agaatgttcg gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg
    15841 gaaagcactt tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg
    15901 ctgcgtaccg cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg
    15961 gtcagatcat tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt
    16021 tcagaccatt ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc
    16081 gttgatttac cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc
    16141 aggttgagat ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc
    16201 agcgcgttac ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg
    16261 gaggctttca cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg
    16321 cgagatttcg aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc
    16381 gctttcttct tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg
    16441 tactcatcgt agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg
    16501 gtacagacca tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc
    16561 tcgttcagca cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg
    16621 tcgagcagga gaatatccgg atcagcaaac agcgcctgcg ccagaagcac acgcagcttc
    16681 cagccaggag caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca
    16741 agcaacaatt caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact
    16801 tccagatcgg ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg
    16861 tcgcgctcct gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca
    16921 gtgaactctt caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg
    16981 agggaaacgt tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat
    17041 ttaccactac cgttcgcgcc aatcaggccg taacggttgc cgccgccaaa tttgacggaa
    17101 atgttttcaa acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca
    17161 ggcgtatcct gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat
    17221 cctgctcctc gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca
    17281 tctgatttca ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc
    17341 aacccaatgg cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt
    17401 attcgcagcg gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc
    17461 tctgccaacc gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg
    17521 taacactcag ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat
    17581 ggaagcgaac ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc
    17641 gcagcagctg atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat
    17701 gcgtctgtat tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg
    17761 tcagttaggc aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg
    17821 cccgacctgg acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct
    17881 tccggctgtc gttccggcag gtccggataa cccgatgggt ctgtatgcac tctacatcgg
    17941 tcgcctgtat gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca
    18001 tggttgtgtg cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg
    18061 tacccgcgta cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg
    18121 ttatattgaa gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat
    18181 tgtgccaatt accctgacga agaacgtgca aacagtgacc ggtcagccag atgttgacca
    18241 ggttgttctt gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc
    18301 ttcaaaactt aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag
    18361 ttggccggga gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt
    18421 attgaataga gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc
    18481 aaatttgcca gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt
    18541 aataaagacg gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc
    18601 tgcgataaac cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc
    18661 aacacgcctt gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata
    18721 aacaccagca gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag
    18781 cctgccgcca caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag
    18841 acgatataca gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc
    18901 cccgtatggt attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc
    18961 agcatcattg cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca
    19021 gaacgtcccc agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg
    19081 ttgactgcca gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt
    19141 agcgtgatag tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa
    19201 agcagcgccg ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc
    19261 accattttgc gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc
    19321 atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt
    19381 gcaaagggca cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga
    19441 tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca
    19501 aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac
    19561 gggcgatttc cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct
    19621 cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga
    19681 tcatttcaat cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg
    19741 aaactcccag acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt
    19801 cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc
    19861 ggaacccttc aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg
    19921 tacctgcgcg acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat
    19981 ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata
    20041 gctatataat atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac
    20101 gggattagca agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt
    20161 cattctccct ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac
    20221 aaaccgcagc aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc
    20281 tgctttcatg caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc
    20341 aatccccttc gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc
    20401 ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc
    20461 actcttgcag gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg
    20521 gcatatgaat ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg
    20581 gactgcgttc tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag
    20641 tttgctctac accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg
    20701 cgtacaaaaa gttctggtcg gtatcagttc gctggtggcg gcttgttatt tcccttttgc
    20761 tcaggcctac ggcgcgccta acttcaatac attgctggca ttgcactcca ccaatatgga
    20821 agagtcgacc gaaatcctga cgatttttcc gtggtacagc tacatggtcg gcatatttat
    20881 ttttgtgctc ggcgtaatag caatcaggcg aaaaaaagag agtgaaaaag cgcgctggaa
    20941 taccttcgac agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca
    21001 aaacctggcc tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt
    21061 tgctaaggat gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa
    21121 actttccgga atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta
    21181 tgtggtggtg atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg
    21241 ggacaatacc ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc
    21301 cagtggctcc acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc
    21361 acagtttcag gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt
    21421 ttccaaccag ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc
    21481 agatgaagtg tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga
    21541 agcgttactg gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat
    21601 tgttctgcat ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga
    21661 aacctttgtg caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga
    21721 tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta
    21781 cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca
    21841 tgatgataaa tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa
    21901 agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca
    21961 gtggacggga attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa
    22021 agccgggccg atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg
    22081 aaccgatatt ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt
    22141 tatatcacca aagtgattag aagcggtaac caacaccggc aatccaggta cctacgtcaa
    22201 cgctacgaat acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga
    22261 actgcagacc agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt
    22321 attccgtggt ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt
    22381 cgttaatgcg gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac
    22441 cagagcttgc agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc
    22501 tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct
    22561 gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg
    22621 cggtgaaagc cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct
    22681 caaatgtgat tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt
    22741 cgcgatttgt gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat
    22801 ccatctaaat ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg
    22861 tcattcatcc agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt
    22921 tagtcaaaac gggggaaaat tttttcaaca aatgctcaac catcattggg tatatccagt
    22981 acactccacg ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac
    23041 gtaaaatgcc ggtctggtta ccaataatca tattgctcgt tgccatggcg tctattcagg
    23101 gtggagcctc gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc
    23161 tgcgtctggc attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct
    23221 ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt
    23281 atctttttta tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca
    23341 ccggaccact ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg
    23401 tgctggcggt tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg
    23461 atttaaccgg ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa
    23521 gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg
    23581 cagcgttaat tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt
    23641 cggttattcc attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg
    23701 aaatgattgc cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac
    23761 cggcgctggc tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc
    23821 tgctggcgct cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag
    23881 agagcaaaat aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa
    23941 ccatgcagaa tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa
    24001 agcatattta ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat
    24061 atctaactca ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg
    24121 ataggaacag ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac
    24181 tgggacataa catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa
    24241 gcgaccaatc tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta
    24301 gagttgctga atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg
    24361 cactggaaca tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc
    24421 accgcactga tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta
    24481 gctctgggga ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac
    24541 atccacaacg ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat
    24601 gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc
    24661 gcgtctcgcg acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat
    24721 cgctgatggt gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt
    24781 gttttgcagc ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt
    24841 aagtaaattt tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc
    24901 aggttttacg ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc
    24961 cccagaatgg tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca
    25021 tctcgaaaaa tcaatgagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg
    25081 caggggatcg tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc
    25141 actggctgca ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt
    25201 tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg
    25261 ctttgacgtt gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa
    25321 gccgatggat ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct
    25381 ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta
    25441 caaaagcggc ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga
    25501 tctcgacggg aaagtggttg ctgtgaagag cggtactggc tccgttgatt acgcgaaagc
    25561 aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact
    25621 gggcaccaac cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa
    25681 aaccgccggt aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg
    25741 tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac
    25801 cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa
    25861 ataataacgc tacacctgta aaacgcactg gcagttccct ctcccctctg gggagaggat
    25921 tagggtgagg ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac
    25981 accacggtaa caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc
    26041 gcttctgatt gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggcaggcgg
    26101 tctggtaatc ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca
    26161 cgtcgcgctg gtctttattg aagtgatccg cggcacacct atcgtcgtcc aggtgatgtt
    26221 tatctatttc gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc
    26281 ggtggtcacc atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt
    26341 gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga
    26401 aaccattcgc tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa
    26461 ccagtggatc atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact
    26521 gacccgtcag gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc
    26581 cgtggcggtg ctctatctga ttattaccct ggtgctgagc tttattctgc gtcgtctgga
    26641 aagaaggatg aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc
    26701 caggtgctgc ataatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg
    26761 ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc
    26821 tccggcgatc tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg
    26881 attcgccagg aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg
    26941 ctggaaaacg tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa
    27001 aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct
    27061 tccgaacttt ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag
    27121 ccgaaaatga tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa
    27181 gtgctgaagg ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac
    27241 gaaatcggtt ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt
    27301 gcggaagatg gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa
    27361 tttttgcagc acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca
    27421 gattcctctc atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt
    27481 ttctcactgg aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac
    27541 ctgcccacgc ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg
    27601 aaccggcccc ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg
    27661 tgctggataa cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga
    27721 cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg
    27781 tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg
    27841 ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca
    27901 gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga
    27961 ttcgcctgtg cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc
    28021 gtgagcgcag caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc
    28081 tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag
    28141 gcagtcgcac catcgctttc caacaaagtt tgtttctcaa tgcctttgcc ctgattgaat
    28201 ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgtccgttca
    28261 cgattcagga cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga
    28321 taggttatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag
    28381 gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct
    28441 ttcgtaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct
    28501 ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt
    28561 ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg
    28621 gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt
    28681 cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac tatccggagc
    28741 tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg
    28801 tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc
    28861 tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca ctcattcttt
    28921 tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg
    28981 atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgtttcgta
    29041 acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg
    29101 tcaatatcgc gccattgctg gcaggtgccg gagcattagg tctggctatc tcgtttggtt
    29161 cgcaaacgct ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga
    29221 acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc
    29281 gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa
    29341 ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc
    29401 gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattgatgg
    29461 aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct
    29521 taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga
    29581 cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg
    29641 cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct gaaccgccgc
    29701 cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc tgctcgtcgt
    29761 tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta
    29821 ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac
    29881 gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca
    29941 tctttttgat aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa
    30001 agttcagttc atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc
    30061 gcgctgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt
    30121 ttttatggat gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg
    30181 cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc
    30241 tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac
    30301 agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt
    30361 cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga
    30421 gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat
    30481 tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa
    30541 gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc
    30601 tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta
    30661 agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat
    30721 tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg
    30781 catgtcattg atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct
    30841 gtcaattttt tgcgtcattt tcatcgccca ggcgacctac acttatcagg cactacccgt
    30901 tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg
    30961 agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag
    31021 ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat
    31081 aaactggcag aaaaagcgcg cgagcaaggc gcgaaaggtt atgtcattaa ttccgcaggc
    31141 ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc
    31201 attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc
    31261 aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat
    31321 aatgcttatc aaaattatta tcacttgcac gaacactatc acgagattaa cagtggcatc
    31381 gcatccgcag agaggctttc tcgtggcagt gaaaatttca acatataaaa aaagtcacct
    31441 gcaaaatgga aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg
    31501 ccggtctttg tattggcatc acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta
    31561 acgcggatga cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat
    31621 ctgccgatcc gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt
    31681 ctgaacaagt gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc
    31741 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
    31801 atatgcgcgg tgctgatacc tctaacagta tttatattga tggcattcgc gatatcggca
    31861 gcgtctcgcg cgacaccttc aatactgagc aggtcgaagt gattaaaggg ccttccggca
    31921 ccgactacgg gcgcagcgcg ccgacgggct cgatcaatat gatcagcaag cagccgcgca
    31981 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
    32041 tggacgtcaa tcaggtcatt ggtgatacca ctgcggtgcg cctgaatgtg atgggcgaaa
    32101 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttcta
    32161 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
    32221 acaacacgcc agacggcggc attccgacca tcggtttgcc gggttattct gccccatctg
    32281 cgggaacagc ggctctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
    32341 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
    32401 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
    32461 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
    32521 ggacctggtc acgcacggcg aataccaaag atgtgagtaa taaaattctc accaaccaga
    32581 ccaacctgac ctcgacgttc tataccggtt ctatcggtca tgatgtcagt accggcgtgg
    32641 aatttacccg tgaaacgcag actaactacg gcgttaatcc ggtgacgtta cccgcggtaa
    32701 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgtaaacg
    32761 ccaatggtca gacggatacc ttcgcaattt acgccttcga tacgctgcaa atcacccgtg
    32821 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
    32881 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc aactggtgtg gcaaaaggtt
    32941 ctccggtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
    33001 cgctgtatca cctgacggaa aacggtaatg tctatattaa ctatgccgtt tcccagcagc
    33061 ctccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gtcaaccgca
    33121 ccgattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
    33181 ataaacgcct gttgctcacc gccgcgctat tccgtactga tatcgaaaat gaagttgagc
    33241 aaaatgatga cggaacttac tcgcaatacg gtaagaaacg tgtcgaaggc tatgagatat
    33301 ccgtggccgg gaatatcacg cccgcgtggc aggtgattgg cggctatacc cagcaaaaag
    33361 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
    33421 cggagcacgc cttcacctta tggagccagt atcaggcaac agatgatatc tctgttggcg
    33481 cgggcgcacg ctatatcggc agtatgcata aaggttcaga cggcgcggtg ggaacgccag
    33541 cgtttaccga aggttactgg gtcgccgatg ccaaactggg gtatcgggtt aatcgcaatc
    33601 tcgacttcca gctaaacgtt tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
    33661 agagcggcta ccgttatcac ccgggcgagc caagaacctt cttgctcaca gccaatatgc
    33721 atttctgatt cagatgtggg gcgcaggccc cactttttgg agaacttgta tgatgtacca
    33781 cattcccggc gtgttatcgc cacaggacgt cgcccggttt cgcgaacaac tggaacaagc
    33841 cgaatgggtg gatggacgcg tcaccaccgg cgcacaaggt gcgcaagtta agaacaatca
    33901 acaggtcgac acccgcagca cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt
    33961 taaccaacat gctttattct ttgccgcggc cttgccgcgt accctttcca cgccgctgtt
    34021 taatcgctat cagaacaatg aaacctatgg tttccatgtg gatggcgcag tacgcagcca
    34081 tccacaaaac ggctggatgc gtactgacct ttctgccacg ctgtttttaa gcgatccaca
    34141 aagctacgac ggcggcgaac tggtcgttaa tgacaccttc ggacaacatc gggtaaaact
    34201 cccggcaggc gatctcgtgt tgtatccctc cagcagcctg cattgcgtga cacccgtaac
    34261 ccgcggcgta cgagtggcat catttatgtg gatccagtcg atgatccgtg atgataaaaa
    34321 gcgcgccatg ctgtttgaac tggacaacaa tattcagtcg ctgaaaagcc gctacggcga
    34381 aagtgaagag atcctgtcgc tgcttaatct ttatcataat ctgctgcggg aatggtcgga
    34441 gatctgatgc ttgaactgcc caggttcgtc cctggattag ctacacttaa ctgtacaagc
    34501 attgatatgg ggaggacgat atggcatccg gttgggctaa cgatgacgcc gtcaacgaac
    34561 agatcaacag tacaattgaa gatgcgattg cccgcgctcg gggtgaaatt ccgcgtggcg
    34621 aaagcctgga tgaatgtgaa gagtgcggtg cccccatccc gcaagcccgt cgggaagcca
    34681 ttcctggcgt gcgcttatgc attcattgtc agcaggagaa agatttacaa aaaccagcgt
    34741 atacaggata taatcgcaga ggttcgaaag acagccagtt acgttaacta caactggcga
    34801 gatgcatagc gagtataatt tgtattttgc gtggcattcc gtgcctttaa cgctgctgtg
    34861 actgccacgc ggcataaaac aagtccataa gtacaacaaa taaatggttt atcagtatga
    34921 tagatatcaa tcaatttatt tgaacaaggc ggtcaattct cttcgatttt atctctcgta
    34981 aaaaaacgtg atactcatca catcgacgaa acaacgtcac ttatacaaaa atcacctgcg
    35041 agagattaat tatgaaaact atcaatactg ttgttgctgc tatggctctt tcaactctgt
    35101 catttggcgt gtttgccgcg gaaccggtaa cggcatccca ggcacagaac atgaataaaa
    35161 tcggcgtggt ttctgccgat ggcgcatcca ccctcgatgc cctggaagcg aaactggctg
    35221 agaaagccgc agcagccggt gccagtggat acagcatcac ttccgccacc aacaacaata
    35281 aattaagcgg tactgcggta atttataagt aattcgtagg acggataagg cgtttacgcc
    35341 gcatccggca acagatgcct gatgcgacgc taccgcgtct tatcagtatc tacaaaatcc
    35401 gaaccgttgg acggatcagg cgtttacgct acgtccagca caagtatttc cctgtctgtt
    35461 tgccgacaga cgcatatgct ctaaccctca ttgatcctat gttacccttg tttgcccgtc
    35521 cgccactgga cgggcttttt tttagctggc taactgctga cagaaagcct gcaacgtctc
    35581 ttccggcata ccaatctgcc gcgctgcatc acaaatggcc tgccagcttc ccgcatccag
    35641 tggaatcccc tgcttctgcc gttcgcgacg cgtgttcact tcccactcgc ccggtagcaa
    35701 aatcggctta tcatcatcat gcggcgaggc tttcacccac tcggcaaagg cttcggtctg
    35761 cgcgttacaa tccggcgcgc cgaagagttc cgggttgatg atgatagtgg tcatgcagtt
    35821 aagaatggca tcgggactgg tttgtaacgt ttcctgatgc gtcgttttac cgccagaaag
    35881 cgccccgcca agaatttcac acatcgctgc aagggcgtag cctttatgtt cggcaaaggt
    35941 caacagcgcc cccaacggcg actcctgcat taccgccgga ttggtcgtcg gcacgccgtt
    36001 aacgtcaatc aggcaacctg gcggcacggg gacgccttta tgccaggcga cgcgagtttt
    36061 accaaatgca atggcgctgg tggcgtaatc aagcaacagc gggaaattat ctttacgagg
    36121 gaaaaccaca cagaacggat tggtgccaaa gcggctgtcg cgaccgtgga acggcgcgac
    36181 cattggaata ccgaccacgc taacaaagtg gatagagaca aaccccgccg ctgcacactg
    36241 ctccgcccag taaccgatac ggccgatatg atgcgagtta tgtagcgcca cggcggcaat
    36301 accgtgctta tgcgctttct caatccccag cgccatcgct tcatgtgccg cgacctgacc
    36361 aaatgcgcga tcgccatcga gcgtgaccgc cgcccccgac tctttaacgg ttttggcatg
    36421 atggttaatt tgcaggtgcc cctgactcca ggagcgtaca tagcttggga tcatgccaat
    36481 accatgtgaa tcatgccctg ccaggtttgc cgcgattaaa tgatcggcaa ctaatttcgc
    36541 ttcttgttcc tcgctaccca tctgacgaaa tacagcctga ataaaactgt gcagcgtctg
    36601 agcatcaaag cgatgaccac tttccatggt taactcctgt ttatgttatg tgtttgttgt
    36661 atttttatgc tgcacgccgg gcgtgggaag gtcaataaag atgcaggaat ttcatattgc
    36721 ggtggggtaa aagccggatg acatggctca tccggctgag aaaagaatta aaatgcctga
    36781 ttaacgcgcg ccaggccgtc gctgatagtt gccgcttcac ccgtagccac caggcagcaa
    36841 gccatctgga ttttcagcga ttcgggaatc ggttcgctgc cagcaaggca acgctcaatc
    36901 cactgcgccg tggtttccgg atcttttgct tgtggcagta actcgctacc agcagtgtcc
    36961 tgtttttcat acagcacccg catcccttca cggtcaatga gattgatctg cgggcagcgc
    37021 tgcggattag catacacttc accttcagta ccatgcatta gtagcgcccg cccgccgata
    37081 tcgctaaaga acttcgcgac gcgtccaatg tattccggat gcgaaacgct ggaaagacgc
    37141 agcgcctcac cttcggcaaa tggcgttgcc agtttcgcca gggtatgtgc actgttacgc
    37201 acgcccatcc gccagcgcat cgccagttgt ttttccagcg gcgggcaaaa cgcgccgact
    37261 ggcataaaca ccggttgatg ttcgtcgagc ttcgcctgcg cctgtccgcc gtgcaacgtt
    37321 ggcgtaatac ccatcaattc aaaaatggtt tcagtcagca cgcgggttgg atcttcgcta
    37381 accccgtgaa ccaccacagg aaaaccgagt ttgtgcagga gaatcgccaa caacggcgtc
    37441 aggttggcct gtttacgcgc gccgttgtaa ctggggatga caatcggcat cggcttgcct
    37501 gcaggcggcg tcagcttgat ggtgtgattt tgcatggctt cgtaaaagcc gagcatctct
    37561 gcttcccctt cccctttgat acgcagcgca atcaatacgc cgcccaactc aaggtcaggg
    37621 acttcaccat tgagcatatg ggcatacaga ccgcgcgcag tatcccggtc taaatcgcgc
    37681 gcgtggtttt tcccgcgccc gatctcttta atgattttgc gatagtccat ttacgactcc
    37741 ttacctgact cacatcatta acgccgccga cggcgtgggg atttcgtttt ttctttcttt
    37801 ttaactatac cttcagggac ttccggttgc tctatcggaa ataccggtaa tgcatccagt
    37861 agtcgcttgc cataattttt ggtcagcaag cgtttgtcgt agataaccac ttcgccccag
    37921 caaccgtggc ttcgaatcag tcgcccaacc tgctgaatca ggttaaacga ggcgctcggc
    37981 aggctttgca cctcaaacgg atagcggttg aggcttttca gccattcccc ttcggtgatc
    38041 accaccggac tgtcgatggg cggaaaagcg attttgtgga tatgcacctg gctgagcaga
    38101 tcacctttca aatcaagccc ttcggcaaat gactgtaagc ccaccagcac actacgctca
    38161 ccgttggcaa cgcgtttgcg gtgcagttca actaagcgat aacgcggctg atcgccctgc
    38221 accagcagca tcaggcgtaa atccgtcaca tagtcgagaa agcgctgcat cgcccgtccg
    38281 ctggcaaaca gtaccaacat accgagatgt tttttgctct ccacctgctt acggaaaaag
    38341 gccgccattt cggcaatatg ctgctcttcg ttgtcgatgg aaggctcaaa gcgcatccgg
    38401 ggaataacaa ttttgccctg ttcgcagtgg ttaaaggggg aatccagcgc cacaaaacgg
    38461 tcgcccgctt tctctttcag accactcatc tcctgcaaac gcgaaaaact gttcagcgaa
    38521 cgcaaggttg cggaggtgac aataatgtgc ggaatactgc gccacagcag cctttccagc
    38581 tgatcgctga cacgtattcc cacgcagtga aaccagagat gcagctgccc ttcgcgctct
    38641 tcccgcgtcg cccatttggt caccggcgca ccggaagatt gcgccagaga cgccagccgc
    38701 cagagtttgc tttgcgcctc gaacatcccc aacgcgcggt tcatctgcaa aatcaaccga
    38761 tgcagacgca caatatcgtg gctgccggtt ttctcactta aatcgttaag aaataactcc
    38821 gccagaccac gcagcatctc ggtgagtttt gccagccgct ggcagatctc cagcacttca
    38881 tcaggcagtt cgcccatcgc aaaacggtgc tctgcctcct gcccggcagg catgtagaga
    38941 ttgagaatgt tgtttaacga ggcgataagc tcatacaact cttcacaatg cgcattcaaa
    39001 cgttcaggga tcgccagcgg tgggatggtc ttcgggcgaa actgctccat gcaggtagcg
    39061 accagtttcg tgaacaagtc cagctgtagt cgataccagg gcgcggtgat ttcggcactc
    39121 atctccagcg catcccgggc cacatctggc aggtgatgac cttcgtccag caccagcagc
    39181 agatttttcg ggtcaggtag tacggcttcg ctttccatcg ccgccatcac cagcgcatgg
    39241 tttgccacca ccacttccgc ttcctgaatc tcccgacgag caacaaaaaa cgtgcattca
    39301 cgatagtagt aacagttgcg gttgaggcag ctggctttgt cggtacttaa acgacgccag
    39361 agatcgtcat ctatagcgat agatgtatga tcgcgcagac catcccattt ataagtgtcg
    39421 agatcgccct tcagcttcgc acaacgtttt tgctcttcct gattatttgg cgtcagttcg
    39481 tcgtcaagaa acgccagcag atcctgttgc gtgggctcag tgctggcgag cgccgtcaga
    39541 ttacgcgggc aaacgtagcg cccacgccca aaagcggcag tgaatttaag atcgggaatg
    39601 atctttttca gcagcggtaa atctttgctg taaatctgat cctgcaatgc cacgttggcg
    39661 gtactcacca ccagcgtttt ttgctcttcg cgggcaatgg cgatgccggg gatcaaatag
    39721 gagagcgttt tcccaacgcc ggtgggggct tcaatcgcca gatgtcgccc ttcttctccg
    39781 gccagcgttt tggcgacgtc cgcaatcatc tgccgctgcg gcgcacgggg aataaagtcg
    39841 gggatctgtt cctgaagcgc cttataccag gcggcaattt gcgctttaag cgcggcggtt
    39901 aatgccatga gaaaacctga aatactgtat aaacagccaa tattgtggca tttttcggtt
    39961 ttcaggggta atggtttttc tgtttctgga aagcggctcg caataaccac caacataatg
    40021 cggtttaatg cccgttcgag cacaaaggat ccaacacgtc atgcaagaca ccatcataaa
    40081 tttctacagc accagcgatg actacggtga tttttctaac tttgccgcct ggcccatcaa
    40141 ggttgacggg aaaacctggc ccacctcaga acactatttt caggcacaaa aattccttga
    40201 tgaaaaatac cgcgaagaga tccgtcgggt ttcttcccct atggtcgccg cacgcatggg
    40261 gcgcgatcgt tctaagcctc tgcgtaaaaa ctgggagtcg gtcaaagaac aggtgatgcg
    40321 aaaagcactt cgcgctaaat tcgaacagca tccagaactg cgtgcgctct tgctggcaac
    40381 cgcgcccgca aaactggttg agcatacgga aaacgatgct tactggggag acggtggtaa
    40441 tggtaagggc aagaatcgac tgggctacct tttaatggag ttgcgcgaac aattggctat
    40501 agagaagtaa cgtctcggtg gcttatgggc ggtaaaacag ataaattttt tcccgtgttc
    40561 tcaaattgct gacaagtgct tgttgtgaaa tgccggatgc ggcgtaaacg ccttatccgg
    40621 cctacaaaag tatgcaaatt caatatattg cagagttcac gtagatactg ataagcgtag
    40681 cgcatcaggc agttttgcgt ttgttcatca gcctgatgcc gggcatagcc cggcataaaa
    40741 gattactgcg cagcggtagg tttacgcgga cggcggcgac gttgttgttc gcctgccggt
    40801 ttggcatcgc cgaggcggcg agacggtttt tctgcaggtt tcgcgcttgc agattttgcg
    40861 ccaccttccc cacggcgtgg ttgctgttga ccgcgaccac caccttgccc acgaccgccg
    40921 ccgccacgtt gctggcgacc gttctggatc ggttcggctt tgattgacgg gtccggctca
    40981 tagcccggaa tcgcaatgcg cgggatctct tttttcagca gtttttcgat atcacgcagc
    41041 agtttgtgtt catcaacaca caccagcgac aacgcttcac cggtagcagc cgcacgaccg
    41101 gtacgcccga tacggtggac ataatcttcc ggtacgtttg gcagttcata gttgacgacg
    41161 tgcggcaact cttcgatatc cagaccgcgc gcagcgatat cggtcgccac cagtacgcaa
    41221 atgtcacccg atttaaaatc agccagcgca cgggtacgcg caccttgcga tttattcccg
    41281 tggatcgccg cactacggat gccatcttta ttgagctgct cagccagatg gttagcgccg
    41341 tgtttggtac gggtaaacac cagcacctgc tgccagttcc ctttaccaat catgtgcgac
    41401 agcaattcgc gtttgcgttt cttatcaaca aagtgaacgt gctgggtcac ctgatcagac
    41461 gcggtattgc ggcgtgccac ttcgatttcc agcgggttgt gcaacagttt ttccgccagg
    41521 gctttaatat cgtcagagaa ggtcgcagag aacagcaggt tctggcgctt cgctggcagt
    41581 tttgttaaca cgcgacggat atcgtggata aagcccatgt cgagcatgcg gtcagcttca
    41641 tcgaggacga ggatttcaac ctgatccagc ttcactgcat tttgatgttc caggtccagc
    41701 aaacgtcccg gtgttgccac cagaacatca acgccgccgc gcagcttcat catttgcggg
    41761 ttaatgctga caccgccaaa caccaccagc gaacgaatgt tcaaatattt gctgtaatca
    41821 cggacgtttt cgccaatctg cgcagccagt tcacgggtcg gggtaagaat gagcgcacgt
    41881 accggacgac gccctttggc gtgcggctgg cgagtgatca ggtgttgcaa cagcggcagc
    41941 gtaaagcccg ctgttttacc ggtaccggtc tgggcgctag ccatcaggtc gcggccttcc
    42001 agcaccgcag ggatcgcctg ctgctgaata ggggtgggtt cacggtaacc ctgctcggca
    42061 acggcgcgca ggatatcagg gcttaaaccc aaagaatcga aagacataac tactccgaac
    42121 cgccccgacc gttaccggtg tagttttcag ggagatactg aaaaggaaaa tgacaaaaac
    42181 cacaatgtca tgagggagcg gagtgtagca gtttttgtga cgcagcgcat aaattatccc
    42241 tgtgactggc gtagctaaaa ttcttagcca tactggacaa ctcccatatg gagtcataat
    42301 cttaatcaat catttgatta agaatggact gtgcgatgaa taatcctgcc atgacaatca
    42361 agggtgaaca ggcgaaaaaa cagctgattg ctgccgcact ggcgcagttt ggtgaatatg
    42421 gaatgaacgc caccacccgc gagatagccg cccaggccgg gcagaatatc gctgccatca
    42481 cctactactt cggttcgaaa gaagatttgt acctcgcctg cgcccagtgg attgccgatt
    42541 ttattggcga gcagttccgt ccgcacgccg aagaggccga acgcttgttc gcacaaccac
    42601 agcctgatcg cgcagccatc cgtgaactga tccttcgcgc ctgcaggaac atgattaagc
    42661 tgctcaccca ggatgacacc gtcaacctca gcaagtttat ctcccgtgag cagctctctc
    42721 ccacggcagc ctatcacctg gtgcacgaac aggtgattag cccgctacac agccacctga
    42781 cacgcctgat tgccgcctgg accggctgcg acgccaacga tacccgcatg atcctccata
    42841 ctcatgcgtt gattggtgag attctggcgt tccgtcttgg caaagaaaca attctgttac
    42901 gtaccggctg gaccgcgttc gatgaagaaa agaccgaact gatcaaccag acggtgacct
    42961 gtcatatcga cctgattttg caaggattat cgcaaaggag tttgtagtga tgaaaaaacc
    43021 tgtcgtgatc ggactggcgg tagtggtact tgccgccgtg gttgccggag gctactggtg
    43081 gtatcaaagc cgccaggata acggcctgac gctgtatggc aacgtggata ttcgtacggt
    43141 gaatcttagt ttccgtgttg gggggcgcgt tgaatcgctg gcggtggacg aaggtgatgc
    43201 tataaaagcg ggccaggtgc tgggcgaact ggatcacaag ccgtatgaga ttgccctgat
    43261 gcaggcgaaa gcgggtgttt cggtggcaca ggcgcagtat gacctgatgc ttgccgggta
    43321 tcgcgatgaa gaaatcgctc aggccgccgc agcggtgaaa caggcgcaag ccgcctatga
    43381 ctatgcgcag aacttctata accgccagca agggttgtgg aaaagccgca ccatttcggc
    43441 aaacgacctg gaaaatgccc gctcctcgcg cgaccaggcg caggcaacgc tgaaatcagc
    43501 acaggataaa ttgcgtcagt accgttccgg taaccgtgaa caggacatcg cccaggcgaa
    43561 agccagcctc gaacaggcgc aggcgcaact ggcgcaggcg gagttgaatt tgcaggactc
    43621 aacgttgata gccccgtctg atggcacgct gttaacgcgc gcggtggagc caggcacggt
    43681 cctcaatgaa ggtggcacgg tgtttaccgt ttcactaacg cgtccggtgt gggtgcgcgc
    43741 ttatgttgat gaacggaatc ttgaccaggc ccagccgggg cgcaaagtgc tgctttatac
    43801 cgatggtcgc ccggacaagc cgtatcacgg gcagattggt ttcgtttcgc cgactgctga
    43861 atttaccccg aaaaccgtcg aaacaccgga tctgcgtacc gacctcgtct atcgcctgcg
    43921 tattgtggtg accgacgccg atgatgcgtt acgccaggga atgccggtga cggtgcaatt
    43981 cggtaacgag gcaggacatg aatgatgccg ttatcacgct gaacggcctg gaaaaacgct
    44041 ttccgggcat ggacaagccc gccgtcgcgc cgctcgattg taccattcac gccggttatg
    44101 tgacggggct ggtggggccg gatggtgcag gtaaaaccac gctaatgcgg atgctggcgg
    44161 gattactgaa acccgacagc ggcaatgcca cggtgattgg ctttgatcca atcaaaaacg
    44221 acggcgcgct gcacgccgtg ctcggctata tgccgcagaa atttggtctg tatgaagatc
    44281 tcacggtgat ggagaacctc aatctgtacg cggatttgcg cagcgtcacc ggcgaggcac
    44341 gtaagcaaac ttttgctcgc ctgctggagt ttacgtctct tgggccgttt accggacgcc
    44401 tggcgggcaa gctctccggt gggatgaaac aaaaactcgg tctggcctgt accctggtgg
    44461 gcgaaccgaa agtgttgctg ctcgatgaac ccggcgtcgg cgttgaccct atctcacggc
    44521 gcgaactgtg gcagatggtg catgagctgg caggcgaagg gatgttaatc ctctggagta
    44581 cctcgtatct cgacgaagcc gagcagtgcc gtgacgtgtt actgatgaac gaaggcgagt
    44641 tgctgtatca gggggaacca aaagccctga cacaaaccat ggccggacgc agttttctga
    44701 tgaccagtcc acacgagggc aaccgcaaac tattgcaacg cgccttgaaa ctgccacagg
    44761 tcagcgacgg catgattcag gggaaatcgg tacgtctgat cctcaaaaaa gaggccacac
    44821 cagacgatat tcgccatgcc gacgggatgc cggaaatcaa catcaacgaa actacgccgc
    44881 gttttgaaga tgcatttatt gatttgctgg gtggtgccgg aacctcagaa tcgccgctgg
    44941 gcgcaatatt acatacggta gaaggcacac ccggcgagac ggtgatcgaa gcgaaagaac
    45001 tgaccaagaa atttggtgat tttgccgcca ccgatcacgt caactttgcc gttaaacgtg
    45061 gggagatttt tggtttgctg gggccaaacg gcgcgggtaa atcgaccacc tttaagatga
    45121 tgtgcggttt gctggtgccg acttccggcc aggcgctggt gctggggatg gatctgaaag
    45181 agagttctgg taaagcgcgc cagcatctcg gctatatggc gcaaaagttt tcgctctacg
    45241 gtaacctgac ggtcgaacag aatttacgct ttttctctgg tgtgtatggc ttacgcggtc
    45301 gggcgcagaa cgaaaaaatc tcccgtatga gcgaggcgtt cggcctgaaa agtatcgcct
    45361 cccacgccac cgatgaactg ccattaggtt ttaaacagcg gctggcactg gcctgctcgc
    45421 tgatgcatga accggacatt ctgtttctcg acgaaccgac gtccggcgtt gatccgatta
    45481 ctcgccgcga attctggcta catatcaaca gcatggtaga gaaaggcgtc acggtgatgg
    45541 tcaccaccca ctttatggat gaagcagaat attgcgaccg catcggcttg gtgtaccgtg
    45601 ggaaattaat tgccagcggc acgccggatg atttgaaagc gcagtcggcc aacgacgagc
    45661 aacccgatcc caccatggag caagccttta ttcagttgat ccacgactgg gataaggagc
    45721 atagcaatga gtaacccgat cctgtcctgg cgtcgcgtac gggcgctgtg cgttaaagag
    45781 acgcggcaga tcgttcgcga tccgagtagc tggctgattg cggtagtgat cccgctgcta
    45841 ctgctgttta tttttggtta cggcattaac ctcgactcca gcaagctgcg ggtcgggatt
    45901 ttactggaac agcgtagcga agcggcgctg gatttcaccc acaccatgac cggttcgccc
    45961 tacatcgacg ccaccatcag cgataaccgt caggaactga tcgccaaaat gcaggcgggg
    46021 aaaattcgcg gtctggtggt tattccggtg gattttgcgg aacagatgga gcgcgccaac
    46081 gccaccgcac cgattcaggt gatcaccgac ggcagtgagc cgaataccgc taactttgta
    46141 caggggtatg tcgaagggat ctggcagatc tggcaaatgc agcgagcgga ggacaacggg
    46201 cagacttttg aaccgcttat tgatgtacaa acccgctact ggtttaaccc ggcggcgatt
    46261 agccagcact tcattatccc cggtgcggtg accattatca tgacggtcat cggcgcgatt
    46321 ctcacctcgc tggtggtggc gcgagaatgg gaacgcggca ccatggaggc tctgctctct
    46381 acggagatta cccgcacgga actgctgctg tgtaagctga tcccttatta ctttctcggg
    46441 atgctggcga tgttgctgtg tatgctggtg tcagtgttta ttctcggcgt gccgtatcgc
    46501 gggtcgctgc tgattctgtt ttttatctcc agcctgtttt tactcagtac cctggggatg
    46561 gggctgctga tttccacgat tacccgcaac cagttcaatg ccgctcaggt cgccctgaac
    46621 gccgcttttc tgccgtcgat tatgctttcc ggctttattt ttcagatcga cagtatgccc
    46681 gcggtgatcc gcgcggtgac gtacattatt cccgctcgtt atttcgtcag caccctgcaa
    46741 agcctgttcc tcgccgggaa tattccagtg gtgctggtgg taaacgtgct gtttttgatc
    46801 gcttcggcgg tgatgtttat cggcctgacg tggctgaaaa ccaaacgtcg gctggattag
    46861 ggagaagagc atgtttcatc gcttatggac gttaatccgc aaagagttgc agtcgctgct
    46921 gcgcgaaccg caaacccgcg cgattctgat tttacctgtg cttattcagg tgattctgtt
    46981 cccgttcgcc gccacgctgg aagtgactaa cgccaccatc gccatctacg atgaagataa
    47041 cggcgagcat tcggtggagc tgacccaacg ttttgcccgc gccagcgcct ttactcatgt
    47101 gctgctgctg aaaagcccac aggagatccg cccaaccatc gacacacaaa aggcgttact
    47161 actggtgcgt ttcccggctg acttctcgcg caaactggat accttccaga ccgcgccgtt
    47221 gcagttgatc ctcgacgggc gtaactccaa cagtgcgcaa attgccgcca actacctgca
    47281 acagatcgtc aaaaattatc agcaggagct gctggaagga aaaccgaaac ctaacaacag
    47341 cgagctggtg gtacgcaact ggtataaccc gaatcttgac tacaaatggt ttgtggtgcc
    47401 gtcgctgatc gccatgatca ccactatcgg cgtaatgatc gtcacttcac tttccgtcgc
    47461 ccgcgaacgt gaacaaggta cgctcgatca gctactggtt tcgccgctca ccacctggca
    47521 gatcttcatc ggcaaagccg taccggcgtt aattgtcgcc accttccagg ccaccattgt
    47581 gctggcgatt ggtatctggg cgtatcaaat ccccttcgcc ggatcgctgg cgctgttcta
    47641 ctttacgatg gtgatttacg gtttatcgct ggtgggattc ggtctgttga tttcatcact
    47701 ctgctcgaca caacagcagg cgtttatcgg cgtgtttgtt tttatgatgc ccgccattct
    47761 cctttccggt tacgtttctc cggtggaaaa catgccagta tggctgcaaa acctaacgtg
    47821 gattaaccct attcgccact ttacggacat taccaagcag atttatttga aggatgcgag
    47881 tctggatatt gtgtggaata gtttgtggcc gctactggtg ataacggcca cgacagggtc
    47941 agcggcgtac gcgatgttta gacgtaaggt gatgtaactt cttatctttc gccagcaaag
    48001 acactaccgc cgggccggca aggattgcca gccctgcaat tgccagcaaa aagttgtttt
    48061 ccagtacacg ggacaacagc cacagcacaa tcagcgctgc gccaaaatac caggtcgtgg
    48121 tcagctcttc cagcacgtct gccacctcac gccagcgttg ctcggggtgg cgctgaaaca
    48181 ccaacatcat cactaccgtg acacaataca gtgcgcataa tcccacgccg acgtgaacca
    48241 gagtcttact catccagccc accaccagca gcgccactac cagtaaatgc aacataatct
    48301 gccagcaact tagaccagtt gcgacacgaa cacgttgttg ccacttcatg gcttctctcc
    48361 tgaagaattt atctctactt actcagagta ccgaacttta cggaaaattc cgtaagaccg
    48421 cacctttttg tgacgccgac tacactattt caatgagata agaggcaaag acaggaaaat
    48481 tatgcccgat caaacacaac aattttcgtt caaggtgctc accatcaaca ttcacaaagg
    48541 ttttaccgcg tttaaccgac gcttcatttt gccggaactt cgcgacgcgg tgcgtaccgt
    48601 cagcgccgat attgtttgcc tgcaggaagt gatgggcgcg cacgaagttc atccgctgca
    48661 tgtggaaaac tggcccgata cctcgcacta cgagtttctc gccgacacga tgtggagcga
    48721 ttttgcctac ggtcgcaatg ccgtataccc ggaagggcat cacggcaacg ccgtactgtc
    48781 gcgttatccc attgaacatt atgagaatcg cgatgtttcg gtcgatggtg cggaaaagcg
    48841 cggcgtgctc tactgccgca ttgtgccacc gatgaccgga aaagcgattc atgtgatgtg
    48901 cgtacatctg ggcctgcgtg aggcgcaccg tcaggcgcag cttgcgatgc tcgccgaatg
    48961 ggtgaatgag ctaccggacg gcgaaccggt attggtggcg ggtgatttca atgactggcg
    49021 gcaaaaagct aatcatccgt taaaagtgca ggccggactg gatgagattt ttacccgcgc
    49081 ccacggacgc ccggcgcgca cgtttccggt gcaatttcct ctactacgac tggacaggat
    49141 ctacgtcaaa aatgccagcg ccagcgcgcc aaccgcgttg ccgctgcgga catggcgaca
    49201 cctttctgat catgcccctt taagtgcgga gattcattta tgaaatgtag ctggcgcgaa
    49261 ggcaataaga tccagttgct ggaaaacggc gagcaatatt atcccgcggt gtttaaggcg
    49321 attggtgagg cacaagaacg catcattctt gaaacgttta tctggtttga ggatgacgtc
    49381 ggcaaacagc tgcatgcggc actactggca gcagcgcaac gcggggttaa agcggaagtc
    49441 ttgctggatg gctacggttc gccggatctc agcgatgagt ttgtcaatga actgacggca
    49501 gctggcgtgg tatttcgcta ctacgatccc cgccctcgcc tttttggtat gcgcaccaat
    49561 gtgtttcgcc ggatgcatcg caaaattgtg gtgatcgacg cgcgtatcgc ctttattggc
    49621 gggctgaatt actccgccga gcatatgtcc agctacggtc cagaggctaa acaggattac
    49681 gcggtacgcc ttgaagggcc gattgtcgaa gatattctcc agtttgagct ggaaaacctg
    49741 cctggacaga gcgcggcacg acgctggtgg cgacgtcatc acaaagcgga agagaaccgc
    49801 cagccgggag aagcgcaggt attgctggtc tggcgcgata acgaagaaca tcgcgatgat
    49861 attgaacgtc actatctgaa aatgctcact caggcgcggc gagaagtcat tatcgccaac
    49921 gcctacttct tccccggcta tcgattttta cacgccttgc gtaaagcggc acggcgcggg
    49981 gtgcggatca aactgatcat tcagggcgaa ccggatatgc cgattgtcag agtcggtgcg
    50041 cgcttgctgt ataactatct ggttaaaggc ggcgttcagg tatttgagta ccgccgccgc
    50101 ccgctccacg gcaaagtggc attgatggac gatcactggg cgacagtagg gtccagtaat
    50161 ctcgatccgc tcagtttgtc actgaatctc gaagcaaatg tcatcatcca cgatcgtcat
    50221 tttaaccaga cgctgcgcga taatctgaac ggcattattg ccgcagattg tcagcaggtc
    50281 gatgagacca tgctgccgaa acgcacctgg tggaacctga ccaaaagcgt gctggcgttc
    50341 cactttttac gccacttccc ggcgctggtt ggctggcttc cggcacacac accacgtctg
    50401 gcgcaggttg atccgcccgc acaaccgaca atggaaacgc aggatcgggt agaaactgaa
    50461 aacacggggg taaaaccctg atgagtaaat cacacccacg ctggcgctta gcaaagaaga
    50521 tcctcacctg gctgtttttt atcgcggtga ttgtgttgct ggtggtctac gccaaaaaag
    50581 tggactggga agaggtctgg aaggtcatcc gcgactacaa tcgcgttgcg ctgcttagtg
    50641 cggtcgggct ggtggtcgtc agctatctga tttacggctg ctatgacctg ctcgcccgct
    50701 tttactgcgg tcacaaactg gcgaagcgcc aggtgatgct ggtgtcgttt atctgctacg
    50761 ccttcaacct gacgctcagt acctgggtcg gcggcattgg tatgcgctat cgtttgtact
    50821 cccgactagg gttaccgggc agcactatta cgcggatttt ctcactcagt attaccacca
    50881 actggctggg ctatatttta ctggcaggga ttatctttac cgcaggcgtg gtggagttgc
    50941 cggatcactg gtatgtcgat caaactacgc tgcgtattct cggcattggc ttactgatga
    51001 ttatcgcggt ttatttgtgg ttttgcgctt tcgcgaagca ccgccatatg accatcaaag
    51061 ggcaaaagct ggtgctgcct tcatggaaat tcgccctcgc tcagatgctg atttccagcg
    51121 ttaactggat ggtaatgggg gcgattatct ggctgttact tggtcaaagc gtgaactatt
    51181 tctttgtact gggcgtgtta ctggttagta gtattgctgg cgtcatcgtg catattccgg
    51241 cggggatcgg tgtgctggaa gcggtgttta tcgcactact ggctggggag catacctcga
    51301 aagggagcat tatcgccgcc ctgctcgctt atcgcgtcct gtattacttt atcccgctgc
    51361 tgctggcgct gatctgttac ctgctactgg aaagccaggc gaagaagcta cgggcgaaaa
    51421 atgaagcggc gatgtgaagt ttagttaagt tctttagtat ctgcatttac gactaatgaa
    51481 aaaaacgcgt atgcctttgc cagacaagcg ttatagctat cgcggcaagt atcccaaaga
    51541 agccgataaa atagtaatag agtgttaatg cacctgtaac agcgcaacga accggactcc
    51601 acaactcttc actatggcat ttccccgcca attcaaggag cttatttcgt ccatgcgaga
    51661 taatgcctga ccataacaca atcattaagc aacttattac ccaaacgggt cgttcactca
    51721 ttaatgcggc attcatgatg atcaccaggg ccaaccctgt caacgtcatc ataatgataa
    51781 aacggtgaga accaggatca acattaaata ataaaccggc cagcatactg attaaaaaca
    51841 tcgcgcccgc agtgctgaac acgacgataa tggcaggtac aggagctaaa cttgcaacga
    51901 caatcgccac caaagagagt aaaaacagat atattacgcc tgcgatgacg gcatttatct
    51961 tgttgatatc cttacttaag ccatacatac aaactaataa taaccctatg gcgacataca
    52021 ttgttggtga atcatatttg gttgccgtaa tttcgagata cttagctccc caggccagtc
    52081 ctgcggttgc caccaaacct aaagcggtaa tgagccatat accgtttaat atcctggtct
    52141 cgtgctgaaa atctaattta ttgctgtttt gtgagtatga ttccattctt ttagttgtcc
    52201 ttagaaaaaa tattaatatt caccatcaat aaatttaaaa ccacgcatac aaaatacttt
    52261 tgtttttatg gatacggaaa acgcttccgc tcgccataag gcgtatttta tacctggtac
    52321 ttgttaagtg tcgtttttag taagaaaaaa aatcccgaca catggccggg atattgatgg
    52381 tgaaaaagaa ttaacggcgg ttgccaaaaa ttcgcagcaa catcaggaac aggttgatga
    52441 agtccagata caaggttaac gcgccaagaa tggaatattt gcgcaggttc gacgtgtcgc
    52501 gggtatcaat ctgctcaccc atatttttca atttctgagt gtcatacgcc gtcaatccga
    52561 caaagacaat cacgccgatg taggtaactg cccacatcaa tgcttcgctt ttcagccaga
    52621 agttgaccag cgatgccagc acaatgccga ttaacgccat aaacagcata ttgccaaagc
    52681 cacttaaatc gcgcttcgtg gtgtaaccgt acaggctcat tgcgccgaac atcccggcag
    52741 taacgacgaa agtactggcg atagaagcag cggtatagac aatgaatata ctggaaagcg
    52801 taagacccgt cagcgccgaa taaagcataa agagcatcgt cgttacacct gcgctcagct
    52861 tttgaatcat cgctgataac acaataacca atgctaattg cgcgatgatc agaccgatta
    52921 aaaagacacg gttagtaaac aacagctcca tcacggccgc ggaattagcc gcataccagg
    52981 caacaaatgc ggtcagcaac aagccaacgg tcatccagcc atagacttgc gccatataag
    53041 tttgcaagcc agcccggggt tgtacgattg aatcagaacg tgggaatctg tccatgacga
    53101 tctcctgaag atataaggaa tatcttaagg atactgcaaa atgatgaggc tgtgcatcga
    53161 cgcagcgtaa acgcatgtac tgagcggtga aattgccgga cgcagcggtg ccttatccgg
    53221 ctaacaaaaa actaccagcg ttttgccgcc tgctgatcgc tctcccgagc ttcaacccag
    53281 cggtcgcctt ccggcgtggc ttcgcgcttc cagaacggtg cgcgggtttt gagataatcc
    53341 ataataaact gcccggcttc aaacgcactg ctgcgatgcg cactggtgac accgacaaaa
    53401 acgatttcat cgcccggcca taattccccg atgcggtgaa tcacagtgac gcgccccagc
    53461 ggccagcggt tacgcgcttc atcaacaatt tctgccagtg ctttttcagt catccccgga
    53521 tagtgttcga gggttaatgc tttgacgctg tcgcccaggt tatggttgcg caccttacca
    53581 gtaaaggtga ctaccgcacc gtcttcgtca cgctccgcca gccacgggta ctcttctcct
    53641 acgctgaacg gctgcggacc aacaacaatt ttggtttctg ccatcttaac ctccggttac
    53701 cggcgggaag aaagctactt cgtcgccgtc agtcagcgga tggtcaaaac tcaccagcgt
    53761 ctggttgacg gcagccagta atttgccatc ttccagcgcc agcgcccagc gatcgctctg
    53821 cgcagccatg tgctggcgta acgcttcaac agttgggaaa tccgcagcca cttcggttgc
    53881 atctgttccc accaactcgc gcacctgggc gaaaaaaaga actttaatca tccgcttcca
    53941 ccttaaagtc acccgacttg ccgccgctct tcgccagcaa acgtaccgga ccaatcacca
    54001 tatctttttg caccgctttg cacatgtcat aaatggtcag cgccgccacg gaggccgcgg
    54061 ttaatgcttc catttcgaca ccggttttcc cggtcaggcg gcataaggtt tctatacgca
    54121 cccgattgtg ctccggctcg gcctgtaaat tgacttcaac tttgctgagc atcagcggat
    54181 gacagagcgg gatcagatcc caggtgcgtt ttgccgcctg aataccggca atacgcgcag
    54241 tggcaaatac gtcgcctttg tggtggcgac catcaataat catcgccagc gtctcgctgc
    54301 gcatggtgac aaaggcttcc gcccgcgctt cacgcacggt ttccgctttg gcggagacat
    54361 ccaccatgtg cgcttcgcca gcggcgttga tatgggtcag ttgcgacata cttatttctt
    54421 caaatgtgga tggaaattac acggacgcgt acgggcatcc agctgcggcg cgatgatatt
    54481 ttcccatgcg gtacggcacg ctttggtcga acccggcatg gcgaaaatca gcgttttgtt
    54541 ggcgacgccc gctaccgcac gagattgcaa cgtggaagtg ccaatctctt caaacgacaa
    54601 catacggaac acttcaccaa aaccttcaat ttcacggtcg aacaacggca gcaatgcttc
    54661 gggagcctga tcaccttccg tcaggccagt accacccgta atcaatacca cttgtacatc
    54721 gtcgctggcg atccacgcag atacctgagc gcgaatagcg tagcggtttt ctttcacaat
    54781 ggctttatcg acaacgtgat ggcccgcttc ttgcgccgaa tcgcgcagat agtgaccgga
    54841 ggtatcgtct tcttcaccgc gacgattaga aaccgtaaga atagcaatac gggtcgggat
    54901 aaattcagtg cttacctgac tcatctgatc tctccttttg acgttttagc cgccaatgta
    54961 cgataagttt tgcgtaatac cggtgttgtt ttgatgcagg aaatgggtct gtttcttctc
    55021 ccgcagcgcc gctgaaatac gcgcttccag cgcctgttgc tgggtatcgt cttccagcag
    55081 atcgcgcagg ttaacgccgc cttcaccaaa caggcagaga tggagtttac caatggagga
    55141 aacgcgcagg cggttgcaag tggcgcagaa gtctttttca tacggcatga taaggccaat
    55201 ctctccggcg taatctggat ggcaaaagac ttgcgcggga ccgtcgctgc gttgacgtaa
    55261 ttggtggatc cagccgcgac gcagtagctc gtcacgcaga acctgaccag agatgtgatg
    55321 cttacggaag agctcgctgc cctcgcccgt ttccatcagt tcgatgaaac gcagctggat
    55381 agggcgatgc tggatccagt tcagaaaggt gtcgagctgg tgatgattaa catcacgcat
    55441 cagcacggta ttgactttga ccttctcaaa accggcctca aatgcagcat caatccccgc
    55501 catgacctgg ttgaatttat cctgcccggt aatagcgtga aactggcggg cgtccagact
    55561 gtcgacactg acgttaatgc cagtaagtcc cgcatcgcgc cagttcgcca catcgcgttc
    55621 cagacggtaa ccattggtgg tgaccgcaat ctggcggata gcgtcgtttt cccgcacagc
    55681 ggcgatgata tcggtaaagt cgcggcgtaa agacggctca ccgcctgtca ggcgcacttt
    55741 ttcggtgccc agactggcga aggcgcgcgt aacccggcga atttcatcga cggtaagaaa
    55801 gcctttattg gtgacgccgc tcggtttgta gccatccggc aggcagtagg tgcaacgaaa
    55861 gttacacaca tcggtaatcg acaggcgcaa gtagtaaaac ttacgcgcaa atgcatcagt
    55921 cagttgtgaa gccatgtaca cctttccaga tacgggaggc gaagtcattt cttccttcgc
    55981 cctggtggca atctttacga gcattgtcac ggccaaagca ccgtatcagt tgacccaggt
    56041 gcagaggcta gagtgtttag tggttatgcc gatactagcg tgtaaatgag atttttacca
    56101 tccacacttt cgctatgtaa tcatgtatat agcgtcatga tcgtgctatt tcgctacaga
    56161 ataagggaaa atggcggttt tactttgata tacatcatca gggaggcata gcgtgcatcg
    56221 tcctttagag gtagaagaaa atagtttgta tcctcaatat tggcaggtta attgctgttt
    56281 ccccgcaatt tgcgctactg tagcgcggat aatctgactc caggaaacta tatgcgcaat
    56341 cgtacgctgg ctgatcttga tcgtgtcgtt gctctcggcg gagggcatgg actgggacgc
    56401 gttctctcat cactttcgtc tttgggttct cgtttaacgg gtatcgtcac caccaccgat
    56461 aatggtggct cgacggggcg tattcgccgt tcagaaggcg gcattgcctg gggcgatatg
    56521 cgcaactgcc tcaaccagct gataacggaa ccgagcgtcg cctccgcgat gtttgaatac
    56581 cgttttggtg gcaatggcga actttccggt cataatctcg gaaacttgat gttaaaggcg
    56641 ctggatcacc ttagcgtgcg gcctctggaa gccatcaatt taattcgtaa tctgctgaaa
    56701 gtggatacgc atttgattcc aatgtcagag catcctgttg atctgatggc gattgacgat
    56761 caggggcatg aagtttacgg cgaggtcaat atcgaccagt taactacgcc gattcaagag
    56821 ttattgttaa cgcctaatgt acccgcaacg cgtgaggcgg ttcacgctat caatgaagcg
    56881 gatctcatca ttattgggcc tggcagtttt tataccagcc tgatgccaat tctgctgctg
    56941 aaggaaatcg cccaggcatt acgccgcacg ccagcgccga tggtttatat cggcaatctg
    57001 gggcgtgagt tgagtttacc tgcggctaat ttgaagctgg aaagcaagct ggcaattatg
    57061 gagcagtatg ttggtaaaaa ggtcattgat gcggtcatcg tcgggccaaa agtggatgtc
    57121 tcggcggtga aagagcggat tgtgatccag gaggtactgg aggccagcga tattccgtat
    57181 cgtcatgacc gccagttgtt acataacgcg ctggaaaagg cgttacaggc tttaggttaa
    57241 cggaaatccg aaggaaaatt ccggcttcct attgaagaca aagtgcgcgt tgtttatgcc
    57301 ggatgcggcg tgaacgcctt atccggccta caaaccgcgc aaattcaata tattgcggag
    57361 aaaatgtagg cctgataagc gtagcgcatc aggctgtttt ccgtttgtca tcagtcttct
    57421 tcgctatcct gttacgatgc cgcgataaac agctcacgca gctgatgcaa ctggtcacga
    57481 atttgcgccg cttcttcgaa ctccagattc tgcgcgtgtt gcatcatcaa cccttccagc
    57541 tcatggattt tctgctgcaa cgctttaggc gacatatcca tcggcacatt atccggctca
    57601 acaatcggtc gcgattttcc tctgcccttc gctttggttt tagcaatgtt ctgccccaac
    57661 gccaggatat cgaccacttt cttgttcaag ccttgcggcg taatgccgtg ttcctcgttg
    57721 tacttctgct gtttctcgcg gcgacgttcg gtttcgccaa tcgctttcgc cattgatggg
    57781 gtgatcttat cgccgtagag aatcgcttta ccgttaacgt tacgtgccgc acgaccaatg
    57841 gtctggatca acgaacgttc ggaacgcagg aagccttctt tgtcagcgtc gaggatcgcc
    57901 accagcgaaa cttccggcat atccagacct tcacgcagta agttgatccc taccaatacg
    57961 tcgaactcac ccagacgcaa gtcgcggata atctccatac gctcgacggt gtcgatatct
    58021 gagtgaagat aacgcacgcg ctcaccgtgt tcttcgagat attcggtaag atcttccgcc
    58081 attcgcttgg tcagtgtggt gaccagtacg cgttcgttaa ttgccgctcg ctgacgaatc
    58141 tccgaaagaa gatcatcaac ctgtgtcgcc accggccgca cttcgataat cgggtcaagc
    58201 aatccggttg gacgcaccac ctgatccacc acatcgccgc cggatttttc cagctcgtaa
    58261 ttacccggcg tcgccgaaac atagatggtt tgcggcgcta atgcttcgaa ctcttcaaac
    58321 ttaagcggac ggttatccag cgctgatggc aggcggaagc cgtactccac cagtgtctct
    58381 ttacgcgccc ggtcaccgcg atacatgccg ccaatttgtg gaatggtgac gtgagattca
    58441 tcgaccacca gcagcccatc agcagggagg taatcaaaca gcgtcggcgg tggctcaccc
    58501 ggtccacgac cggagaggaa gcgcgagtag ttttcaatcc ccgaacagta gcccagctcg
    58561 ttcatcatct ccagatcaaa ctgggtacgc tgggtcagcc gctgctcttc cagcagtttg
    58621 ttgttttcca acagcacttt gcgtctggcg gccagctctt ctttgatctc ctccatcgcc
    58681 tgtacgatgc gctcgcgcgg tgtgacgtag tgcgttttcg ggtagatggt aaaacgtgga
    58741 atagtggaaa caatctgccc ggtcagcggg tcaaataacg acaatcgttc cacttcctcg
    58801 tcaaacagtt ccacgcgaag tgcaatgtca tccgattctg ccgggaagat atctatcacc
    58861 tcgccacgaa cgcggaaagt accacgctgg aatgcttgat cattacgagc gtattgcagc
    58921 tccgccagtc ggcgcagaat cgcgcgctga tcgataatca taccgaccgt gagatggagc
    58981 atcatcttga gatataaatc aggatcgccc agaccataaa tcgcggaaac agacgccacc
    59041 acaaccacat cacgccgctc cagcatcgct ttggtggcgg acaaacgcat ctgctcaata
    59101 tgttcgttaa ccgaggcatc tttctcaatg aaagtgtcgg aactcggtac ataggcttcc
    59161 ggctgatagt agtcgtagta ggaaacgaaa tattccaccg cgttttccgg gaagaactct
    59221 ttcatttcgc catacagctg ggccgccagc gttttgttgg gcgcaagtac catggttggg
    59281 cgctgaaggt cagcaatgac attggcaatg gtgaatgttt tcccggagcc agtcacgcca
    59341 agtaacgtct ggtgcgccag gccatcttcc agcccctctt cgagacgtcg aatcgcctct
    59401 ggctgatcgc cagaaggttt aaaagcggaa ttcagtttga acggtttact catgagtcgc
    59461 tacctgaagg agttgggcgg gcaggtatgt aattttactc gtcgtactta attatgccaa
    59521 caaattatac tggataaaaa aacagttcat caccataata tttctgttac agcgtaaact
    59581 ccgctgtcaa tcatgagcaa aatttactct gtggcgagat aaaactccgg ccttaccggg
    59641 ttatccccaa agcaacggct tttttaacat ttgtcaagat gagtgatgac agttttatgg
    59701 caagagatgc ctgttcagtg actgccattg catttatcta accagttaaa aaataaaaga
    59761 tatttctttg agccgtcttt aacgccaatt cgcgtgcaag ccgcgtattc tctcgcttgc
    59821 ctcgtgtttt ctaactctaa tacacatggt tatccacagg aatagtggat aactgtctcc
    59881 agcccctatc ccccgccgcc tgggcaattc ccacattcgc cgcatgaggc ggaagtaaaa
    59941 tttttttcgt gattatttta gaatttaatt ggttaaattg cagtcaatcg aagacgcgat
    60001 ctcgctcgca atttaaccaa atacaggata gctacaacaa ggcaaggttt atgtattttc
    60061 cggttgccgc attttctgga ttttctgcaa gccaggggat ctctcccagc agcggcgcgg
    60121 gaatcatgcg ggtgagcgtg gtcatatatt cagcgtgacg ttttcccgga ggcgtaacat
    60181 cgttcgccac ccaacccgcc agagtcagtc cggcgtgttg tattgcctgc gcagtcaaca
    60241 tcgcgtgatt aatacagccg agtttcacac caactaccag tatcaccggc agttgttcct
    60301 gtgttaccca atctgcaaaa gtgaaagtgt cagaaagcgg cgtaaaccag ccgccagcac
    60361 cttccactaa cacccagtca gcctgttgtt caagcgcgcg taatccggcg ctcattacca
    60421 atgattctat cggcctgccc tcttgcgcgc tgatgatgtg cggcgaggtg ggttctgcga
    60481 aggtgtaagg atttaccgtt gcgtaatcca gctgcaggct gctgttgtgc tgtaacgcca
    60541 gcgcgtcact attacgtaag ccttccgggg tcttttcgct gccagaggcg accggtttat
    60601 aacctgccgt ccgataacct actgccttcg cggcttgtaa aagtgcacaa ctggcgacgg
    60661 ttttccccac ttcggtatcc gttccggtga caaaataacg tttactcacg agcaatcact
    60721 cccaaaaaaa gatgatacgt cagaggatat cgcccctgtt gttgcggcca ggccagttgc
    60781 aatcgctgca attgcgaacg cgttaatatt cgcgggtcgc gcccttcatg aagatgcgta
    60841 gcaccgatgc ctttcagcga acgcatggca ctgagcgcat catcaaacca tagtgtgatg
    60901 ggctgaatat gatgttgata atgcacgccg ttcagcgact gttcgatttc atctggcggt
    60961 aaaaagcgat tagcatgcgg acgctcgtcc accgcctgcc acgcctgatg cagttcgggt
    61021 aacgatccct gcaccagcgt ggtaaacgcc accacgcctc cggggcgcac cacccgatac
    61081 agctcgcgga gtgcagtgga taaattaccg caccactgca ctgcgagatt gctccatgca
    61141 agatcgaacg tcgcagtcgc taacggcagg gattcgatat ctcccgccag ataatggtct
    61201 gcggcatcct tctggcgtgc ctgaacaagc attggcggcg agagatctaa ggccgtcacc
    61261 tgcgcgtgac gttcccgcca gtggcggctc atccagccag gtccacaacc cgcgtccagt
    61321 acgtgggtgt atttacgctg tgggagcatt gccagtaaga cgtcagcact ctggcgctgt
    61381 agatctgcgt gttgctcata gagtgcggct gcccgaccaa atgccgctgc aatggcttgt
    61441 ttattaaccg ttgccatgca gcacctccag cagacggtcg atatcctgca tttcatgcgc
    61501 tgcggttagc gttaagcgca gtcgcgcagt accagcgggt acggttggcg ggcgaatcgc
    61561 cgtgacccag cagccttgct ggcgcagttt ttctgccagt tgtaacgcac ggctgttatc
    61621 accgacgatc aatggctgga tggcgctgca tgaatcagcc agcgtaaacg gcaaatcctg
    61681 tactccggca cgaaaacgcg caatgagtga caccagtttt tcgcgccgtg catccccctc
    61741 atcacggcga atgaccgcca gtgacgcacg taatgcctgc gcctgagcgg gcggcatact
    61801 ggtgctgtag ataaggtggc gggcgaattg cagcagataa tccgccaccg tactggagca
    61861 aagcaccgca gccccactga cgccaaatcc tttgccaaaa gtcaccacca gcaattctgg
    61921 ttttaccttt tgcagccagc agctgccgcg cccctgctcc ccgataacgc ccgtgccgtg
    61981 ggcatcatcg accatcaacc agccattgtg ctgttgcgtt acctgctgga tttccgccag
    62041 tggcgcaata tcgccgtcca tgctgaacac gccttctgtc accaccagtt gctgccccgg
    62101 acagggggaa gcaagcagtc gcgccagatg agtgacatcg ttatgaacaa aacggcgaag
    62161 ctgcgacggg cttaaactgg cagcctccag caatgaggca tggctaagcc ggtcagcgac
    62221 aatgcggtcc tcttttgcca tcatcgcggc aatgaccgcc tgattagcgg caaaaccaga
    62281 gataaatagc agtgcccgcg aatagcccag ccactcggcc agttcttctt ccagcgcctg
    62341 atgcgcaacg ctataaccgc tgacgtgacc ggaaccgccg ctaccgacgc caaattgctc
    62401 cgcaccctgc tgccaggcac ggataatttg cggatgatgg cttaaaccta aataatcgtt
    62461 actggaaaag ttcagatact ggcgatcatc cgccaccagc cagcgtccgg ctccttgcgc
    62521 cactggataa cggcgacgca gggcatcggc agcacgccgc gcatcgagcg ccgcgttgat
    62581 tttctcctgc cagctcataa tgctgccgcg ttgtaatatt cgtcggtgtc cggggtcatc
    62641 agcgcctgtt caagacgttg ctgttgttcg ttatcccctg ccagcacggc agtttgctgc
    62701 ggatttagcc ccagtttgcg gaacagttgc aggtctttat cttcttccgg attcggcgtg
    62761 gtcagcagtt tgcaaccgta gaaaatcgag tttgcgcctg ccataaagca catcgcctga
    62821 gtctgttcgt tcatctgctc gcgtccggca gaaaggcgca cgtaagaggt cggcatcatg
    62881 atccgcgcga ccgcaatggt gcgaataaaa tcaaaggcat cgacatcatc gttatccgcc
    62941 agcggcgtgc ctttcacctt caccagcatg ttgattggta cgctttccgg cggcgtcggc
    63001 aggtttgcca gttgcagcaa taatccggcg cgatctttta ccgtttcgcc taagcccaca
    63061 atgccgccag aacagacttt gatcccggca tcgcgcactt tttccagcgt atcgaggcgt
    63121 tcctgataag tgcgggtggt gatgatattg ccgtaaaact ccggcgaggt gtcgaggttg
    63181 tggttgtagt aatccagccc ggcattcgca aggcgctgcg cctgagattc actcaacgtg
    63241 cccagcgtca tacacgcctc cagccccatc gcttttaccc cctgcaccat ttgttccagg
    63301 tacggcatat cgcgttcgtg gggattcttc cacgccgcgc ccatacagaa acgcgtcgat
    63361 cccgcagctt tcgctttgcg cgccgactcc agcacctgtt caacttccat caaccgctcg
    63421 gcttccagcc cggttttgta gcgcgagctt tgcgggcagt atttgcaatc ttccggacaa
    63481 gctccggtct taatcgacag caaagtgctg acctgcacct gacgaggatc gaaatgctgt
    63541 cgatgcacct gctgcgcttc aaacagcaga tccagcaacg gtttttcaaa taattctgtg
    63601 acttgcgaca atgtccagcg tgggcggtga gccatggggc ttctccaaaa cgtgtttttt
    63661 gttgttaatt cggtgtagaa ttgtaaacct aaatcttttc aatttggttt acaagtcgat
    63721 tatgacaacg gacgatcttg cctttgacca acgccatatc tggcacccat acacatccat
    63781 gacctcccct ctgccggttt atccggtcgc gagcgccgaa ggttgcgagc taattttgtc
    63841 tgacggcaga cgcctggttg acggtatgtc gtcctggtgg gcggcgatcc acggctataa
    63901 tcacccgcag cttaatgcgg cgatgaagtc gcaaattgat gctatgtcgc atgtgatgtt
    63961 tggcggtatc acccatgcgc ccgccatcga gctatgccgc aaactggtgg caatgacgcc
    64021 gcaaccgctg gaatgcgttt ttctcgcgga ctccggctcc gtagcggtgg aagtggcgat
    64081 gaaaatggcg ttgcagtact ggcaggccaa aggcgaagcg cgccagcgtt ttctgacctt
    64141 ccgcaatggt tatcatggcg atacctttgg cgcgatgtcg gtgtgcgatc cggacaactc
    64201 aatgcacagc ctgtggaaag gctatctgcc agaaaacttg tttgctcccg ccccgcaaag
    64261 ccgcatggat ggtgaatggg atgaacgtga tatggttggc tttgcccgcc tgatggcggc
    64321 gcatcgtcat gaaatcgcgg cggtgatcat tgaaccgatt gtccagggcg caggcgggat
    64381 gcgcatgtac catccggaat ggttaaaacg aatccgcaaa atgtgcgatc gcgaaggtat
    64441 cttgctgatt gccgacgaga tcgccaccgg atttggtcgt accggcaaac tgtttgcctg
    64501 tgaacatgca gaaatcgcgc cggacatttt gtgcctcggt aaagccttaa ccggcggcac
    64561 aatgaccctt tccgccacgc tcaccacgcg cgaggttgcg gaaaccatca gtaacggcga
    64621 agccggttgc tttatgcatg ggccaacttt tatgggcaat ccgctggcct gcgcggcagc
    64681 aaacgccagc ctggcgattc tcgaatctgg cgactggcag caacaggtgg cggctattga
    64741 agtacagctg cgcgagcaac ttgcccccgc ccgtgatgcc gaaatggttg ccgatgtgcg
    64801 cgtgctgggg gcaatcggcg tagtcgaaac cactcatccg gtgaatatgg cggcgctgca
    64861 aaaattcttt gtcgaacagg gtgtctggat ccggcctttt ggcaaactga tttacctgat
    64921 gccgccctat attattctcc cgcaacagtt gcagcgtctg accgcagcgg ttaaccgcgc
    64981 ggtacaggat gaaacatttt tttgccaata acgagaagtc cgcgtgaggg tttctggcta
    65041 cactttctgc aaacaagaaa ggagggttca tgaaactcat cagtaacgat ctgcgcgatg
    65101 gcgataaatt gccgcatcgt catgtcttta acggcatggg ttacgatggc gataatattt
    65161 caccgcatct ggcgtgggat gatgttcctg cgggaacgaa aagttttgtt gtcacctgct
    65221 acgacccgga tgcgccaacc ggctccggct ggtggcactg ggtagttgtt aacttacccg
    65281 ctgatacccg cgtattaccg caagggtttg gctctggtct ggtagcaatg ccagacggcg
    65341 ttttgcagac gcgtaccgac tttggtaaaa ccgggtacga tggcgcagca ccgccgaaag
    65401 gcgaaactca tcgctacatt tttaccgttc acgcgctgga tatagaacgt attgatgtcg
    65461 atgaaggtgc cagcggcgcg atggtcgggt ttaacgttca tttccactct ctggcaagcg
    65521 cctcgattac tgcgatgttt agttaatcac tctgccagat ggcgcaatgc catctggtat
    65581 cacttaaagg tattaaaaac aactttttgt ctttttacct tcccgtttcg ctcaagttag
    65641 tataaaaaag ctgaacgtga aacgctaaag accattaata tcaatgtgtt acaatatatt
    65701 tagtctaaaa aatagactgc ataatactac aaaacacaac atatctagta actatgaatc
    65761 aactacttag atagtattag tgacctgaga cagagcatta gcgcaaggtg attttatgtc
    65821 ttcttgcgct aattttttgt cataaaaaca tgtggcagac tttgtatatc tctgctttgc
    65881 cacgccacac catcatgcat tatggctatg cctaaacaga atcaataaga atagaacat
//
LOCUS       DAZUFB010000014        65563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZUFB010000014 DAZUFB010000000
VERSION     DAZUFB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.014"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..621
                     /gene="srlA"
                     /locus_tag="ACHY3M_001050"
                     /gene_synonym="gutA"
     CDS             58..621
                     /gene="srlA"
                     /locus_tag="ACHY3M_001050"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4316009.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            618..1577
                     /gene="srlE"
                     /locus_tag="ACHY3M_001051"
                     /gene_synonym="gutE"
     CDS             618..1577
                     /gene="srlE"
                     /locus_tag="ACHY3M_001051"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4316010.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1588..1959
                     /gene="srlB"
                     /locus_tag="ACHY3M_001052"
                     /gene_synonym="gutB"
     CDS             1588..1959
                     /gene="srlB"
                     /locus_tag="ACHY3M_001052"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4316011.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1963..2742
                     /gene="srlD"
                     /locus_tag="ACHY3M_001053"
     CDS             1963..2742
                     /gene="srlD"
                     /locus_tag="ACHY3M_001053"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFS4316012.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2847..3206
                     /gene="gutM"
                     /locus_tag="ACHY3M_001054"
     CDS             2847..3206
                     /gene="gutM"
                     /locus_tag="ACHY3M_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4316013.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3273..4046
                     /gene="srlR"
                     /locus_tag="ACHY3M_001055"
                     /gene_synonym="gutR"
     CDS             3273..4046
                     /gene="srlR"
                     /locus_tag="ACHY3M_001055"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFS4316014.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4039..5004
                     /gene="gutQ"
                     /locus_tag="ACHY3M_001056"
     CDS             4039..5004
                     /gene="gutQ"
                     /locus_tag="ACHY3M_001056"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFS4316015.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5001..6515)
                     /gene="norR"
                     /locus_tag="ACHY3M_001057"
     CDS             complement(5001..6515)
                     /gene="norR"
                     /locus_tag="ACHY3M_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFS4316016.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6702..8141
                     /gene="norV"
                     /locus_tag="ACHY3M_001058"
     CDS             6702..8141
                     /gene="norV"
                     /locus_tag="ACHY3M_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFS4316017.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCCEHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            8138..9271
                     /gene="norW"
                     /locus_tag="ACHY3M_001059"
     CDS             8138..9271
                     /gene="norW"
                     /locus_tag="ACHY3M_001059"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFS4316018.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9497..11749)
                     /gene="hypF"
                     /locus_tag="ACHY3M_001060"
     CDS             complement(9497..11749)
                     /gene="hypF"
                     /locus_tag="ACHY3M_001060"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFS4316019.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11902..12429)
                     /gene="hydN"
                     /locus_tag="ACHY3M_001061"
     CDS             complement(11902..12429)
                     /gene="hydN"
                     /locus_tag="ACHY3M_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFS4316020.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12578..13588)
                     /gene="ascG"
                     /locus_tag="ACHY3M_001062"
     CDS             complement(12578..13588)
                     /gene="ascG"
                     /locus_tag="ACHY3M_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFS4316021.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            13848..15305
                     /gene="ascF"
                     /locus_tag="ACHY3M_001063"
     CDS             13848..15305
                     /gene="ascF"
                     /locus_tag="ACHY3M_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFS4316022.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            15314..16738
                     /gene="ascB"
                     /locus_tag="ACHY3M_001064"
     CDS             15314..16738
                     /gene="ascB"
                     /locus_tag="ACHY3M_001064"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFS4316023.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16852..17271)
                     /locus_tag="ACHY3M_001065"
     CDS             complement(16852..17271)
                     /locus_tag="ACHY3M_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4316024.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17268..17579)
                     /locus_tag="ACHY3M_001066"
     CDS             complement(17268..17579)
                     /locus_tag="ACHY3M_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFS4316025.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(17753..18496)
                     /locus_tag="ACHY3M_001067"
     CDS             complement(17753..18496)
                     /locus_tag="ACHY3M_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316026.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKFV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(18539..19009)
                     /gene="hycI"
                     /locus_tag="ACHY3M_001068"
     CDS             complement(18539..19009)
                     /gene="hycI"
                     /locus_tag="ACHY3M_001068"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFS4316027.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(19002..19412)
                     /gene="hycH"
                     /locus_tag="ACHY3M_001069"
     CDS             complement(19002..19412)
                     /gene="hycH"
                     /locus_tag="ACHY3M_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFS4316028.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(19409..20176)
                     /gene="hycG"
                     /locus_tag="ACHY3M_001070"
     CDS             complement(19409..20176)
                     /gene="hycG"
                     /locus_tag="ACHY3M_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFS4316029.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(20176..20718)
                     /gene="hycF"
                     /locus_tag="ACHY3M_001071"
     CDS             complement(20176..20718)
                     /gene="hycF"
                     /locus_tag="ACHY3M_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFS4316030.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20728..22437)
                     /gene="hycE"
                     /locus_tag="ACHY3M_001072"
     CDS             complement(20728..22437)
                     /gene="hycE"
                     /locus_tag="ACHY3M_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFS4316031.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22455..23378)
                     /gene="hycD"
                     /locus_tag="ACHY3M_001073"
     CDS             complement(22455..23378)
                     /gene="hycD"
                     /locus_tag="ACHY3M_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFS4316032.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23381..25207)
                     /gene="hycC"
                     /locus_tag="ACHY3M_001074"
     CDS             complement(23381..25207)
                     /gene="hycC"
                     /locus_tag="ACHY3M_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFS4316033.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25204..25815)
                     /gene="hycB"
                     /locus_tag="ACHY3M_001075"
     CDS             complement(25204..25815)
                     /gene="hycB"
                     /locus_tag="ACHY3M_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFS4316034.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25940..26401)
                     /gene="hycA"
                     /locus_tag="ACHY3M_001076"
     CDS             complement(25940..26401)
                     /gene="hycA"
                     /locus_tag="ACHY3M_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFS4316035.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26613..26963
                     /gene="hypA"
                     /locus_tag="ACHY3M_001077"
     CDS             26613..26963
                     /gene="hypA"
                     /locus_tag="ACHY3M_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS4316036.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26967..27839
                     /gene="hypB"
                     /locus_tag="ACHY3M_001078"
     CDS             26967..27839
                     /gene="hypB"
                     /locus_tag="ACHY3M_001078"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFS4316037.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27830..28102
                     /gene="hypC"
                     /locus_tag="ACHY3M_001079"
     CDS             27830..28102
                     /gene="hypC"
                     /locus_tag="ACHY3M_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFS4316038.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28102..29223
                     /gene="hypD"
                     /locus_tag="ACHY3M_001080"
     CDS             28102..29223
                     /gene="hypD"
                     /locus_tag="ACHY3M_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFS4316039.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29220..30230
                     /gene="hypE"
                     /locus_tag="ACHY3M_001081"
     CDS             29220..30230
                     /gene="hypE"
                     /locus_tag="ACHY3M_001081"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFS4316040.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30304..32382
                     /gene="flhA"
                     /locus_tag="ACHY3M_001082"
     CDS             30304..32382
                     /gene="flhA"
                     /locus_tag="ACHY3M_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFS4316041.1"
                     /translation="MSYTPMSDLGQQRLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32419..32772)
                     /gene="ygbA"
                     /locus_tag="ACHY3M_001083"
     CDS             complement(32419..32772)
                     /gene="ygbA"
                     /locus_tag="ACHY3M_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFS4316042.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32849..32983)
                     /locus_tag="ACHY3M_001084"
     CDS             complement(32849..32983)
                     /locus_tag="ACHY3M_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316043.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            33059..35620
                     /gene="mutS"
                     /locus_tag="ACHY3M_001085"
     CDS             33059..35620
                     /gene="mutS"
                     /locus_tag="ACHY3M_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4316044.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35726..36382
                     /gene="pphB"
                     /locus_tag="ACHY3M_001086"
     CDS             35726..36382
                     /gene="pphB"
                     /locus_tag="ACHY3M_001086"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS4316045.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(36433..37200)
                     /gene="ygbI"
                     /locus_tag="ACHY3M_001087"
     CDS             complement(36433..37200)
                     /gene="ygbI"
                     /locus_tag="ACHY3M_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFS4316046.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            37396..38304
                     /gene="ltnD"
                     /locus_tag="ACHY3M_001088"
     CDS             37396..38304
                     /gene="ltnD"
                     /locus_tag="ACHY3M_001088"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFS4316047.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            38301..39563
                     /gene="otnK"
                     /locus_tag="ACHY3M_001089"
     CDS             38301..39563
                     /gene="otnK"
                     /locus_tag="ACHY3M_001089"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFS4316048.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDLARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAESVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39560..40198
                     /gene="ygbL"
                     /locus_tag="ACHY3M_001090"
     CDS             39560..40198
                     /gene="ygbL"
                     /locus_tag="ACHY3M_001090"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFS4316049.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            40203..40979
                     /gene="ygbM"
                     /locus_tag="ACHY3M_001091"
     CDS             40203..40979
                     /gene="ygbM"
                     /locus_tag="ACHY3M_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFS4316050.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            41068..42432
                     /gene="ygbN"
                     /locus_tag="ACHY3M_001092"
     CDS             41068..42432
                     /gene="ygbN"
                     /locus_tag="ACHY3M_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFS4316051.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(42471..42707)
                     /locus_tag="ACHY3M_001093"
                     /pseudo
     CDS             complement(42471..42707)
                     /locus_tag="ACHY3M_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
     gene            complement(42718..44139)
                     /locus_tag="ACHY3M_001094"
     CDS             complement(42718..44139)
                     /locus_tag="ACHY3M_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFS4316052.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGGFYLDKACVVSRDPLDPDNFGKQNV
                     GIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATPLK
                     YDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFTGH
                     YSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFPEV
                     QAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFNLP
                     QVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHYSQ
                     PVVDLPETKAWAEKLTAMLAARK"
     gene            complement(44139..44732)
                     /locus_tag="ACHY3M_001095"
     CDS             complement(44139..44732)
                     /locus_tag="ACHY3M_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFS4316053.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44879..45286
                     /locus_tag="ACHY3M_001096"
     CDS             44879..45286
                     /locus_tag="ACHY3M_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4316054.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(45407..46399)
                     /gene="rpoS"
                     /locus_tag="ACHY3M_001097"
     CDS             complement(45407..46399)
                     /gene="rpoS"
                     /locus_tag="ACHY3M_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFS4316055.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46462..47554)
                     /gene="nlpD"
                     /locus_tag="ACHY3M_001098"
                     /pseudo
     CDS             complement(46462..47554)
                     /gene="nlpD"
                     /locus_tag="ACHY3M_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
     gene            complement(47739..48365)
                     /gene="pcm"
                     /locus_tag="ACHY3M_001099"
     CDS             complement(47739..48365)
                     /gene="pcm"
                     /locus_tag="ACHY3M_001099"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFS4316056.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48359..49120)
                     /gene="surE"
                     /locus_tag="ACHY3M_001100"
     CDS             complement(48359..49120)
                     /gene="surE"
                     /locus_tag="ACHY3M_001100"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFS4316057.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(49101..50150)
                     /gene="truD"
                     /locus_tag="ACHY3M_001101"
     CDS             complement(49101..50150)
                     /gene="truD"
                     /locus_tag="ACHY3M_001101"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFS4316058.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEYI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(50147..50626)
                     /gene="ispF"
                     /locus_tag="ACHY3M_001102"
     CDS             complement(50147..50626)
                     /gene="ispF"
                     /locus_tag="ACHY3M_001102"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFS4316059.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50626..51336)
                     /gene="ispD"
                     /locus_tag="ACHY3M_001103"
     CDS             complement(50626..51336)
                     /gene="ispD"
                     /locus_tag="ACHY3M_001103"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS4316060.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51355..51666)
                     /gene="ftsB"
                     /locus_tag="ACHY3M_001104"
     CDS             complement(51355..51666)
                     /gene="ftsB"
                     /locus_tag="ACHY3M_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFS4316061.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51860..52183)
                     /gene="ygbE"
                     /locus_tag="ACHY3M_001105"
     CDS             complement(51860..52183)
                     /gene="ygbE"
                     /locus_tag="ACHY3M_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFS4316062.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(52233..52838)
                     /gene="cysC"
                     /locus_tag="ACHY3M_001106"
     CDS             complement(52233..52838)
                     /gene="cysC"
                     /locus_tag="ACHY3M_001106"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFS4316063.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52838..54265)
                     /gene="cysN"
                     /locus_tag="ACHY3M_001107"
     CDS             complement(52838..54265)
                     /gene="cysN"
                     /locus_tag="ACHY3M_001107"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFS4316064.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTSGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(54267..55175)
                     /gene="cysD"
                     /locus_tag="ACHY3M_001108"
     CDS             complement(54267..55175)
                     /gene="cysD"
                     /locus_tag="ACHY3M_001108"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFS4316065.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55427..56464
                     /gene="iap"
                     /locus_tag="ACHY3M_001109"
     CDS             55427..56464
                     /gene="iap"
                     /locus_tag="ACHY3M_001109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFS4316066.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56546..56818
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56607..56635
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(56915..57208)
                     /gene="cas2e"
                     /locus_tag="ACHY3M_001110"
     CDS             complement(56915..57208)
                     /gene="cas2e"
                     /locus_tag="ACHY3M_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFS4316067.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57208..58128)
                     /gene="cas1e"
                     /locus_tag="ACHY3M_001111"
     CDS             complement(57208..58128)
                     /gene="cas1e"
                     /locus_tag="ACHY3M_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFS4316068.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(58125..58775)
                     /gene="cas6e"
                     /locus_tag="ACHY3M_001112"
     CDS             complement(58125..58775)
                     /gene="cas6e"
                     /locus_tag="ACHY3M_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFS4316069.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(58757..59503)
                     /gene="cas5e"
                     /locus_tag="ACHY3M_001113"
     CDS             complement(58757..59503)
                     /gene="cas5e"
                     /locus_tag="ACHY3M_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFS4316070.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGAWLSKEEPCTSQE"
     gene            complement(59514..60569)
                     /gene="cas7e"
                     /locus_tag="ACHY3M_001114"
     CDS             complement(59514..60569)
                     /gene="cas7e"
                     /locus_tag="ACHY3M_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFS4316071.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(60581..61120)
                     /gene="casB"
                     /locus_tag="ACHY3M_001115"
                     /gene_synonym="cse2"
     CDS             complement(60581..61120)
                     /gene="casB"
                     /locus_tag="ACHY3M_001115"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFS4316072.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(61117..62679)
                     /gene="casA"
                     /locus_tag="ACHY3M_001116"
                     /gene_synonym="cse1"
     CDS             complement(61117..62679)
                     /gene="casA"
                     /locus_tag="ACHY3M_001116"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFS4316073.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGTAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(62777..65476)
                     /locus_tag="ACHY3M_001117"
     CDS             complement(62777..65476)
                     /locus_tag="ACHY3M_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFS4316074.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIESPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        15491 a        17881 c        16916 g        15275 t
ORIGIN      
        1 ctgattagat taggttgccg tttggtaata aaacaataaa tcctgaagga gagaacaatg
       61 atagaaacca ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa gggcggagag
      121 gtgtttaccg ggatggtgac cggcattctt ccgctgttga ttagcctgct ggttatcatg
      181 aacgcactga ttaattttat cggtcagcat cgtattgaac gttttgctca acgttgcgcc
      241 ggtaaccctg tttcccgtta cctactgtta ccgtgcattg gcacgtttgt cttttgcaat
      301 ccgatgacct taagcctggg tcgctttatg ccggaaaagt acaaacccag ctactacgcg
      361 gcggcctctt atagctgcca ctcaatgaat ggcctcttcc cccatatcaa ccctggcgaa
      421 ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca
      481 ctggcggtga gttatctgct ggttggtctg gtcaccaatt tcttccgcgg ctgggtgacc
      541 gatctgacca ccgccatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcac
      601 ctggcaggag caacatcatg acgcatattc ggatcgaaaa aggaacgggt ggctggggcg
      661 gcccgcttga gctgaaagcc acgccgggaa aaaaaatcgt ctatatcacc gccggtaccc
      721 ggcctgcgat tgttgacaaa ctggcacagc ttactggctg gcaggctatt gacggattta
      781 aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat
      841 tacgctgcgg catctatccg aaacgacgta ttcccaccat taatatccac tcgacgggca
      901 agtccggtcc gctggcgcag tacattgtgg aagatattta tgtctctggc gtaaaagaag
      961 aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc cgtgactatg
     1021 acaccagtaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg ggaatgggca
     1081 tggggtccgc cgttgcggtg ctgtttcaat ctggtcgtga caccatcgac actgtattaa
     1141 aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt atggcttctg
     1201 gccttggtga ctggattgcc cacggtcttg ctccgctggc gagccatcca ctgggtctgg
     1261 tcatgctggc gctcatctgc tccttcccac tgctttcacc tttcctcggc ccaggcgcag
     1321 ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc aatattccgc
     1381 cgcatctggc tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc
     1441 cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg
     1501 tactggtgag ccgcttttta accggcgcac cgactgtact gatcgcctgg tttgtctccg
     1561 gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac catcacccgt
     1621 atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc
     1681 gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt gaaaggtgca
     1741 ctccatcccg gtttgcaatt tacgctcggg cagcatcgct atccggtgac cgctgttggc
     1801 agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta
     1861 aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga cgatatcgcg
     1921 ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt
     1981 gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg
     2041 tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc acaagaaatt
     2101 aacgccgaat atggtgaaag tatggcgtac ggttttggtg ctgacgccac tagcgagcaa
     2161 agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga tttgctggtc
     2221 tatagcgccg gaatagcaaa agcagccttt atcagcgact tccagctcgg cgattttgac
     2281 cgttcgctac aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt ttcgcgtttg
     2341 atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc cggcaaagtg
     2401 ggcagcaaac acaactctgg ctacagcgca gcaaaatttg gtggcgtcgg gctgactcaa
     2461 tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat gctcggtaac
     2521 ctgctgaaat cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa gctgggtatc
     2581 aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg cggctgcgat
     2641 tatcaagatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc gtactgcacc
     2701 ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc ggagatccat
     2761 taaggatctc cgtgagacta tagaatgcct gatgcgctac gctcatcagg catacaggac
     2821 ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc gccgttatcg
     2881 cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg
     2941 acacactatg ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc tttaaaccgc
     3001 gggtcgtggt cgccatcgcg ctggacgatc agcagcgcat cgtcgacacc ttgtttatga
     3061 aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt atgcatgcgg
     3121 gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat
     3181 tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat
     3241 cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca
     3301 ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg caatactttg
     3361 acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca
     3421 ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata
     3481 aaacactcat caacacccac aagaaagagc tgattgcaga agctgccgtt agttttatcc
     3541 atgatggcga ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg gttcccctgc
     3601 tctcgcgctt taataacatc acggtgatga ccaacagcct gcatatcgtc aatgcgctat
     3661 ccgaactgga taacgaacaa actatcctga tgccaggcgg aacgtttcgt aaaaaatcgg
     3721 cctcatttca cggacagctg gcagagaatg ccttcgaaca tttcagtttc gataaattgt
     3781 ttatgggcac cgacggcatc gatctcaatg cgggcgtaac caccttcaac gaggtttata
     3841 ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa
     3901 agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctgatta
     3961 ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga
     4021 tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg
     4081 ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc
     4141 gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc
     4201 cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc tttttttgtc
     4261 catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg
     4321 tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg tctggaagat
     4381 aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggca
     4441 aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc
     4501 tccagcaccg tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg
     4561 cgcggattta atgaagaaga ttttgcccgc tcccacccag ccggggcact gggcgctcgc
     4621 ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg ccatcccaca ggtggcgtta
     4681 accgccagcg tgatggatgc gatgctggaa ctcagccgca ccggtctggg gctggtggcg
     4741 gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg
     4801 ctggttggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac aggcggcacc
     4861 acattacagg cacaaagtcg cgccatcgac gccaaagaga tcctgatgaa gcgcaaaatc
     4921 actgccgcac cggtggtgga tgaaaacggc aaactcaccg gcgcaattaa cctgcaggat
     4981 ttctatcagg ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg
     5041 ttggcgacgt cggtttccag catccgcgcg caggcagccc agttgtgatg attttgtgcc
     5101 agtgcctggc gaatggtttc acgctggaac gcttctgtcg cttcacgcag gttttgctta
     5161 acaacgggca ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt
     5221 tgtgcctcaa gaatcacttc atcgccgctg cgagtggctc tcgccagcac taccgcccga
     5281 tgaatagcat gttccagttc gcgcacgttc cccggaaaac gatagtgttg cagtaaattt
     5341 cgcgctccgg cacttaatac cacgcgggag agccccagcc gcaaacgaca ctgctcgcag
     5401 aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt
     5461 ggaaacacgc tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag cacctcttcg
     5521 cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga cccgcaaact acggtcatcg
     5581 ccaacgcgct gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac
     5641 gacaactcgc cgatctcatc gagaaacaga gtgccgttat ccgccatttc aaacttcccg
     5701 ctgcggttac tgatagcgcc agtaaacgct cctttcacat gcccgaacaa ctcactttcc
     5761 gccacacttt ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc
     5821 gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatc
     5881 agaacgttga gatcggacgc cgccacaatc tcaatctctt ttttcaattg cgtcatgcct
     5941 ggcgacaagc caatcatctg cgtctgtttc accgcttcaa acggcgcggc atcgcccggc
     6001 agcatattct ggctttccag ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc
     6061 agcgcggcaa tcagccgtaa ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc
     6121 ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa acaatggcag accaacgcag
     6181 gcgtgaacct tcagactctc ctgcccagga atcaaaccgt catagggatc gggcaattcg
     6241 ctgtctgcag gaaagcgcac cacgtccccg gcgcgggcaa tcgcttcaag ccgtggatgc
     6301 ccttccagcg caaagcgtct accgagtaca tcctttgcca gaccgtcgat ggcaagcgga
     6361 ataaactgcc gcgaatcgta acgtagcaac gcagacgcat cgcactccag cacctgacgt
     6421 agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg
     6481 gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta
     6541 tagatattgt catatcgacc atttgattga tagtcatttt gactactcat taatgagcat
     6601 aattttattt atagagtaaa aacaatcaga taaaaaactg gcacgcaatc tgcaattagc
     6661 aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa
     6721 ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac ggcacggaat
     6781 ataaaacgct gcgcggcagc agttacaaca gctacctcat ccgcgaagaa aaaaacgtgc
     6841 tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa
     6901 tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgctgggg
     6961 cgctgaccga actgatggcg caaattcccg atacgccgat ctactgtact gccaacgcta
     7021 tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg
     7081 gcgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc
     7141 actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg
     7201 ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc
     7261 ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa
     7321 cgccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctccc
     7381 acggcgtggt ctggcgcgat aacccgacgc aaattgtcga gctgtacctg aagtgggccg
     7441 ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca
     7501 tgatggctga cgctatcgcc cagggaattg cggaaaccga cccacgcgtg gcggtgaaaa
     7561 ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag
     7621 gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg
     7681 tggaggagat gactggttta cgcttccgta acaaacgcgc cagtgctttc ggctctcacg
     7741 gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa
     7801 tgtcgcttag cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgttgtg
     7861 aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga
     7921 atacggtggt taaagaagaa acctctgcca ccacgacggc tgacctcggc ccacggatgc
     7981 agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg
     8041 cgccaggaac gccgtggagt gaagttccgg ataacttcct ctgcccggaa tgctccctcg
     8101 gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg agtaacggca ttgtgatcat
     8161 tggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccactat
     8221 tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaacctg acctcagcca
     8281 tgttatcagt caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc
     8341 cgagcagttt aatctgcgcc tgtttccgca cacctgggtg acggatatcg atgccgaagc
     8401 ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac aagctagtgc tggcaaccgg
     8461 tgccagcgcc tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt taaatagtca
     8521 gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgtcgg
     8581 tggtggcttg attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac
     8641 gctaatcgac aacgctgcca gtattctggc gtcattaatg ccaccggaag taagcagccg
     8701 cttgcagcat cggttgacgg agatgggcgt tcatctgttg ttgaaatctc agttgcaggg
     8761 gctggaaaaa acagattctg gcattctggc aacgctggac cgccagcgca gtatcgaagt
     8821 ggatgcggta attgccgcca ccggactgcg cccggaaacc gccctggcac gacgcgccgg
     8881 gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat
     8941 ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tccagccgat
     9001 tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cactgaaact
     9061 accggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac
     9121 ccagcgtcag gatttacgct ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg
     9181 cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc
     9241 atttggattg ttgaaaacgt tgccgatgta ggtgggctac tgtgcctaaa atgtcggatg
     9301 cgacgctggc gcgtcttatc caacctacgg ggagcgcatg tgttggtcac atactgcgtt
     9361 catgtcgcat ccacctactg tgcccaaaat gtcggatgcg acgctggcgc gtcttatccg
     9421 acctacgggg acgcatgtgt aggccggata aggcgtttac gccgcatccg gcaatggtgt
     9481 ccaaatgcaa cacgttttat ccgttctgga cttcacccgc taaccaacgc gccgcagcaa
     9541 taaccccctg ccccagagac aaaccgccat cacccgccgg taaactctgt ggaaagagca
     9601 atgtgaaatc agcgagataa tgcgccagac gtgcacgcag caaacggtta tgaataaccc
     9661 cgccgctaaa taccagcgta gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg
     9721 caaaaccctg cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc ggtgcctgcc
     9781 agttcagcca ctgctgccag aaagtggcga gatccagttg attgtccacc agcggcattg
     9841 tcaccggatg cgtcactccg tggcacgaag ctgcgagtgc ctccagagca caagccgctt
     9901 caccttcata acttaacgtg gctggcgcac agcccagtgc cgccgccacg gcatcgaaca
     9961 accgcccaca cgatgacgcc agcggcgcgt taattccacg ctcaatggcc cgcgccagca
    10021 cgctccagtt ttgctgttgc acacttgctg tttcagaata attctgccac tccggcacaa
    10081 agcgcaggca ctgcgccagc aggtttcgcc acggctgctt cgccgccaaa tcgccacccg
    10141 gaagcgccac tgcaggcaag ccgcccaggt gctcacattc gcgatagttc acccgcagac
    10201 actcgccgcc ccacaaagcg ccgttctccc ccataccgat accatcgagc gtcaaagcaa
    10261 tgacatcccc gccatccagc ggccactgat gctctgccag acacgccgct gcgtgggcat
    10321 gatgatgcag caccgtttgc gtcggcatat tcatttcgct cgcccactgg ctggagacat
    10381 agcccggatg cacgtcatgc acaacgtatt gcggagtgaa atcgtagatg ttttgcatca
    10441 ggcgtaacgc ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg cccagatgct
    10501 gactcaacac cgcttgttca ccgcgcacca ggcagaaggt gtttttcaga tccgcgccga
    10561 gacacagcac aggcggaaca tttttaaagc ccggaggcaa agccagcgca tccggcacat
    10621 acccccgcga acggcgcagc atttcgccgc tttcgcgcac caccgaatca tccatccgct
    10681 gcacgatgtc gcggttatgt atcaagaatc cgtcggcaat gccctgcaaa tccgccagcg
    10741 cctgttcgtt gctgatagct ggtggtttac cgctcaggtt gccggaggtc atcaccagcg
    10801 ggcattgcag ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac attaccccga
    10861 cttcgttaag gtcaggggcg atatcatcac aaagctcagg aacgtatttt ttatccacca
    10921 gcacaatcgg cgcggcgggc gtggtaagca actggcgcgc agcgtctggt aaaccgtcag
    10981 ccactggcaa catgactgcc agcggtttcg ccgggcggtg tttgcgctcc cgcagtgtcg
    11041 ccaccgcgtt actgttacgt gcatcgcagg caagatgaaa tccgccaatc cctttgatgg
    11101 cgacaatttt gcccattttt aactgtgcga tagctgcctg taatgccgcc tcttgttccg
    11161 catgttcacc atgacttacc cattcaagat gcgggccaca ctccgggcag gccaccggct
    11221 gggcgtggaa gcgacgatcg agcgggtcgc ggtactcttt atcacaggct ggacacagcg
    11281 gaaactccgc cattacggta aacgggcggt cgtaaggcat ggcgcgaata atggtgaaac
    11341 gcgggccgca gtgggtacag ttgataaacg gataacgata acgccgttcg cctggggtat
    11401 tcatttcggc aaggcaagca gggcaagtag cggcatccgg gacaatttgc gtattcatgg
    11461 cacctcccgc gctctggcgg atagtgaact cggtgggcag ttgtgaccag ataaacggct
    11521 cacgctcgac gctatcaata cgcgccagtg gcggacagtg ctgatgcaat tgaacaagaa
    11581 acgtttccgg gtcttccagc agccggactt ctacgccatc gccgtcatta cagacatcgc
    11641 cgtgaagatt taattgctgt gccagctgcc agacaaacgg acgaaaaccg acgccctgca
    11701 ctttgccacg aatacgcagt tggacaccgc aagatgtgtt ttttgccatt gagttattcc
    11761 cgccatcatg aattgcgtaa cccgtccagc cggacacgac agcgtcgcat ccggcagtca
    11821 caggtcggcg ataccgcccg cttcgtattc tacgaatatt tccgggaatt cctttgatgc
    11881 cagaacagtt ctgtaagatt tttagaacat cagcgccgta cggcggcgtt tttctgcgct
    11941 cagttgttca agtttattac gatcgacaca aattagcgca tgggtcgggc aagccgccat
    12001 acacgccggg ccgtcttcac gatggttgca caggtcgcat ttattggctt cagctttgtc
    12061 agcccgtaca ttcagcccca cgccgctgtt gcgaatcacc ggacgtacca ccacttccat
    12121 cgcgccatac gggcaagcca ccacgcaggt tttgcaacca atacaacgtt cctgcatcac
    12181 atgaacaaac cctttatcac ggctgatagc accattcggg cagacgttag cgcacggtgc
    12241 atcttcacac tgacggcaaa ctgtcgccgt ggaaatgttc acacctttaa tgacatggat
    12301 acgcggtaaa aatgtttccg gggtcagcga tgcacagtcc tgattttcct gatgagaaac
    12361 cacgcacgcc acttcacagg tacggcaacc aatacattta ctcgcgtcag caatgatgaa
    12421 acggttcatc aaattctcca gcaatgacag ttaatgcgcc gatacattca caaatcatgc
    12481 cagtttttaa tttactgtta tttaaggaaa ttaatttctg taatgcagga aaaacgatgt
    12541 catcgacact agtgacgatg acatgtgatg acaatgttta tcgcgaaagg gcaatgagtg
    12601 agtcgcggca gatcaatttt ccgctgaagg ttttcggcgg tgagaaatcc ccgccatcga
    12661 gcataaaaat cagccgtcca ataatttcct gaatcatctc agtcaccgga atttttacgc
    12721 tggagagcgc cggaacgata tagggggcaa ttgcgatatc atcgaatccg ataactgaca
    12781 cctgctctgg caccgctacg ccgcgctcgt gcaacgcttt catcgcacct atcgccatat
    12841 cgtcgttact ggcaactaac gcgctaaatt tagccccacg ttcgagcaac atttctaccc
    12901 cttcggcccc gctggcaggc gtccatctac cgttagcgat aagtttttca ttgagcgcaa
    12961 taccgtgctg cgccagcgcg tctttatatc cggcaagacg ttcaacgctg gtgggagaat
    13021 ccatcgaacc ggtaaggaaa gcaatctcct gatgtccggc gtttatcaac tctgccacgg
    13081 cgttaaaact ggtctgttta tgatcgcacc agacgctatg gctgctgttt ttgcgcaggc
    13141 ggcgattaag caccattatc ggctgactgt gcgcgtcaat gatgtcatcg atctcatcca
    13201 cgcttaaaaa acgcgggtaa atcataatgg cgtcgcagcg caaatccagc agatactgaa
    13261 tcgcctggcg ctcttcttcg gcgctgtgtt taccatctgc caatagcaac tgccgccctt
    13321 tctcttccgc cattcgcgcg gcatgaaaca gtaattcact aaaataaatg ccgtggtaaa
    13381 gcgtgttggt cactaccagc cccagcgtct gagtactctt cgccgacaaa ttgcgcgcca
    13441 gcaagtttgg gcggtaaccg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct
    13501 ggctgacgta gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc
    13561 gcttcgccac ttccagcatc gtcgtcatca ttttcatccc tttacacgca atcaacgcag
    13621 tgtactgcac cgtttgccga ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc
    13681 gatttcacaa atataaaaaa tgaacaatta actctcttgc ttatttagtg acaactattc
    13741 atgattttgt gaaaccggtt tcctaattcc gtttcagcat cggcattttt ccgttacgtc
    13801 gactgataac aactacatct accctactga taacaggata aaatccgatg gccaaaaatt
    13861 atgcggcgct ggcacgctcg gtgatagcgg cactgggcgg cgttgataac atctcggcgg
    13921 tcacgcactg tatgacgcgt ttgcgctttg ttatcaaaga tgaccagctt atcgacagcc
    13981 cgacgttaaa aaccatcccc ggcgtgctcg gcgtggtacg tagtgacaac cagtgtcagg
    14041 tgattatcgg caataccgtt tcacaagcct ttcaggaagt cgtcagcctg ctgccgggag
    14101 atatgcagcc cgcacagccc gtgggtaaac ccaaactcac gctgcgtcgc attggtgcag
    14161 ggatcctcga tgcgctgatc ggcaccatgt caccgctgat cccggcgatt atcggcggat
    14221 cgatggtcaa actgctggcg atgatcctcg agatgagcgg cgtgctgaca aaaggatcgc
    14281 cgaccttaac cattctgaat gtgattggtg acggtgcttt cttcttcctg ccgctgatgg
    14341 tcgcggcatc tgccgccatc aaatttaaaa ccaatatgtc gctggcgatt gccatcgcgg
    14401 gtgtgctggt acatccgagt tttattgaac tgatggcgaa agcggcccag ggtgaacatg
    14461 tcgaatttgc cctgattccg gtcaccgcgg tgaaatacac ctacacagtg atcccggcgc
    14521 tggtgatgac ctggtgcctg tcatatatcg aacgctgggt ggatcgcatt acaccggcgg
    14581 tgaccaaaaa cttcctcaag ccaatgctga ttgtgttgat tgccgcaccg ctggcaatcc
    14641 tgctgattgg cccgattggt atctggatcg gtagcgccat ttcggcgctg gtttacacca
    14701 ttcatggtta tctgggctgg ctttcagtcg ccattatggg cgcgctgtgg cctctgctgg
    14761 taatgaccgg gatgcaccgc gtctttacgc caaccatcat tcagaccatt gccgaaaccg
    14821 gcaaagaagg gatggtcatg ccgtcagaga tcggcgctaa cctgtcgctg ggcggttcat
    14881 cactggcggt ggcgtggaaa acgaaaaacc cggaactgcg ccagacggcg ctggctgcgg
    14941 cggcatcagc cattatggcg gggatttccg aaccggcgtt atacggcgtg gcgatccgcc
    15001 tgaaacgtcc gcttatcgcc agtcttatca gcggctttat ttgcggcgcg gttgccggta
    15061 tggcggggct tgccagccac tcaatggcag cgccggggct atttaccagc gtgcagttct
    15121 tcgatccggc gaatccaatg agcatcgtct gggtgttcgc ggtcatggcg ctggcggtca
    15181 tgctgtcgtt tatcctcaca ctgttgctcg gctttgagga tattcctgtt gaggaagcga
    15241 ctgccgaggc gcgaaagcat cagagcgtac aaccgaccgt cgccaaagaa gtaagtctta
    15301 attgaggatg aaaatgtcag tatttccaga aggtttttta tggggcggcg cgcttgccgc
    15361 caaccagtct gaaggtgcgt tccgtgaagg tggcaaaggg ctgaccactg tcgatatgat
    15421 cccacacggc gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt ttcagttgcg
    15481 cgatgacgag ttttatccca gccatgaggc gacggatttt tatcatcgtt ataaagaaga
    15541 tatcgccctg atggcagaga tgggattcaa ggttttccgt acctcaattg cctggagccg
    15601 tctctttccg cagggcgatg aaatcacgcc caatcagcag ggcattgctt tttatcgttc
    15661 tgtctttgaa gagtgtaaaa agtacggtat cgaaccgctg gtcacgttgt gccacttcga
    15721 tgtgccgatg catctggtca ccgaatatgg ctcctggcgt aaccgcaagc tggtggagtt
    15781 tttcagccgc tacgcccgga cctgctttga agcatttgat ggtctggtga aatactggct
    15841 taccttcaat gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg gtctggtgtt
    15901 tgaagaaggt gaaaatcagg atcaggtgaa atatcaggcc gcgcatcacc agctggttgc
    15961 cagtgcgcta gccaccaaaa tcgcccatga ggttaacccg caaaatcagg tgggctgtat
    16021 gctggcgggc ggtaacttct acccttacag ttgcaagccg gaagatgtct gggcggcgct
    16081 ggagaaagac cgggaaaacc tgttttttat cgatgtgcag gcgcggggcg cgtatccggc
    16141 ttactctgcc cgcgtattcc gcgaaaaagg ggtaaccatc gacaaagcac cgggcgatga
    16201 tgaaatcctg aaaaacaccg tcgattttgt ctctttcagc tattacgcct cgcgctgcgc
    16261 ctcggcggag atgaacgcca acaatagcag tgcggcgaac gtagtgaaat cgctgcgtaa
    16321 cccgtatctg caagtgagcg actggggctg gggtattgat ccactcggtc tgcgtatcac
    16381 catgaacatg atgtacgacc gttatcagaa gccgctgttt ctggtggaaa acggcctcgg
    16441 cgcaaaagat gaacttgctg ccaatggcga gattaacgat gactatcgca tcagctattt
    16501 acgcgaacat atccgcgcaa tgggcgaagc gattgcagat ggcattccgc tgatgggcta
    16561 caccacatgg ggctgtattg atttagtttc cgcctctacg ggtgaaatga gcaaacgcta
    16621 cggttttgtc tacgtagacc gtgacgacgc aggcaacggc acgctgacgc gcacacgtaa
    16681 gaaatcgttc tggtggtata aaaaagtgat tgccagtaat ggtgaagatt tagagtaagc
    16741 aacagtgccg gatgcggcgt gaacgcctta tccggcctac atgtcgcacg aactaggaag
    16801 gcccgataag cgcagcgcat cgggcaatct ggggtttggc atcagtcaaa atcaaagaaa
    16861 ccattccggc ttaacgccaa atcgggctga aagtgcctta atgtgggata tcgtcaggga
    16921 tcgttgtcca gacaaaatct gactgaccag ggatttagag ccgatttcgt tcttcaaatc
    16981 cgcataggtc agattatgtt gatcaatcag cgtgcgaagt aacgccacgc caacaggcat
    17041 tgccgcaacg gctttgtcga actctgcaaa cttttcgttg ttgttttcat actctgcaat
    17101 gcggctcgcc agaaagtcga taagtggatt ggtgtcatct tcttcaatca aatattcgac
    17161 cagttccagt gcttcacgat aatcatcttc tgagtcactg cctcccagaa aggggactgc
    17221 attgaccagt tctcttgttg ctttaacagc gcgtgcagca ttagcagtca ttctttgttc
    17281 tctctgtagt aacgtgtcag tttgtcatac tctgcgtgat tcgttatgtg cttcacaaag
    17341 aaccgcttat taacaaagtt tatgtaggca ataaccctaa gattgttccc gccgacatcc
    17401 agaacgtacc atttgttgcg atatctaaaa ttgtcgaggt tgggaaacgc cgttcgcatt
    17461 tcttctggtg tagaaaaatc cgtttcctta ataacccgat acaatgcctg atgagccaga
    17521 gcgtcgttag gatatttacg cgcagactct tcaaatggcg ctttagaaat gatgtgcatt
    17581 tttaagtttt aacttcctta tagtaagtaa acagccacat ctgcgatgtt ctatccttat
    17641 gtgttcggaa actatagctc atcaaatcca agttcacaat atgtaaacac ttgtgcgcga
    17701 tataattacc cccaaaatcc tcatagccac tctgcaaaaa gtcagttctg gttcacccct
    17761 caagcacatc cccctttatc aagcaacgct gcgtctgcgc cagatcttcc gtttgcgggt
    17821 cgcgcccggc gatgggtttg aggacgaatt tacggttggg ggtataagta aaacgcgtga
    17881 gcgcgggata gcttaacttg ccaaaccagc cgtgaacacg ctgccatttg gcgtctttta
    17941 cctgatacag actggttatc cagtaaccag aattaaactg catatccagc aattgcgtgt
    18001 gaccattgcc ttgcaaatcc agcaaatcat ctacgccagt tttgcttgcg gtgtagaatc
    18061 ccctgggttc aaagacacag ggacggcctt cgcgcgtgaa cgtcataaga attaaatgtg
    18121 cataaggagc cagaccatta ccggtacttg gtaaccagat aaccagatcc tgaatgccgt
    18181 tacgatcaag atcggcgcga tagagttgcc ctttgcccgc tatgtttgcc agcataccca
    18241 caggcacagt ccaggattta ccggcgcggt ctttagcggt taattgccag taatcttctt
    18301 catttggcat cttctcaagc gtcacgccag caacgcccac tttcctaaaa tgccaggttt
    18361 gctcggcatc ataaggcatg gtcaggagag gtttcagcgc ccgcttttgc gcgtattcgc
    18421 tgtcactcat tggcagatat tcggtaaagc tggtggcctg tgcaggcagc actaagctca
    18481 ataaaaaagc gcacatccct gttctgatgt tcaattccct gaattccctg atggagaatt
    18541 attcttcttc caccgctaac tgcgcaaatc caccatttcc ttcccagcct tccagtcgtt
    18601 gataaacggt ttctaccgca tctttaatcg gctgggtcat cgggtagtaa aagccgacga
    18661 tatccggctg aatgccgagg aaaatcactt caccaatgtc ttcttttaac tggtcgataa
    18721 ggtaattcaa cggcatgtta tgggtagtca tcataaacat ctcggcgata tcatccgggt
    18781 cgatgatgcg gatttcgccg gggtttagcc ccatatccgt ggcgtcgaca atcagcagtc
    18841 gtgtcgggcg cagttcacgg atggcgacga tgtcgttttc cggtgcacta ccgccgtcaa
    18901 tcaccaccca gttacctttc ggcgcggcgg cgcacttttc cgccagcagc gggccagcgc
    18961 catcatcgcc catcatgcta ttgccaacac agagtaaaac gtcagtcacg cagtctcctc
    19021 accatcaaat agatggcgtt ttcctgatga atatcatgca gcatacttaa cagcattgta
    19081 ctccattcct tctgctgcga agtttgcgct gcccgggctt tatcgaatgc gttggccagc
    19141 atcggcacat ggttaatgtc gatgacgatc tcgccatatt tcggtacgcc ttccattttg
    19201 cggcgggctt cgctgcccgc ctccagagtg gcaatccatg ccagatattc atcccatggg
    19261 caggtgagcg ccgcttccag gcaatcgata accccaaggt ggtgaccaat cgccaggctg
    19321 taatagacca cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg
    19381 ctcagttgac tgaacaccac cttttcactc atcggatacg cgcctcttca acaacatgat
    19441 tcagatggct gacaatctcg ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa
    19501 cctgttcttc gccctgccct aactgtgtaa ggtaatcatc ggcaatctga cgaccgtaac
    19561 gataacccgc caggcgacgt gcttcgcgat ccactttcac gcgcagcggc tgcaccatat
    19621 cgccatgcag aatctcagcc ggttgttcat cctgctcacc cgggccacgg gcgtgaattt
    19681 tctgctccag caggccgagt gccattgcaa agccgtacag cgtggcggca ggcgttggcg
    19741 ggcaacccgg aatgtagaca tctaccggga cgattttatc cgtaccgccc cacacgcagt
    19801 agagatcgtg gaaaatacca ccgctgttac cgcacgcacc gtaggagata caaattttcg
    19861 ggtccggcgc ggactgccac gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc
    19921 cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt
    19981 ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat ttcgcagccg ttgcagccgc
    20041 cgcagtccac gcggtaaaca taggcagaac gtttgatttt tttcagtaac gacgccttca
    20101 tgctggcgat ggattcatcc accgtcatcg ggaccggaat gccgttggcg tcacgggggc
    20161 ctaataaatt gctcatcaga tggcctcttt catatggcga gtcagttcaa tacggtcgga
    20221 cggcaccagg catttctggc gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt
    20281 ttccgcgcgg ctgtcaccat tgtgcttaag cagcgcaatg gcgtagtcga tctctttctg
    20341 gacggcgaaa ggacgattgc agacgcgaca gctgcacagc acaaaacggg actgttgcag
    20401 gaaatcttct ttcttccaca ccgccagttc gtactcttgc gacagtttga tcgctgccgt
    20461 cgggcagact tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa actcccacgc
    20521 cagctcaccg gtggcgaggt cagtttcaac cgttaaggcg tttgacggac aggcattgac
    20581 gcaggccgca cagccgatgc actgctgcgg gttctgctcc ggcttaccac ggaagttttt
    20641 atcaaccgca atcggctcca gcggataaga cgaggtcgcc gtgccggttt tgatgacttt
    20701 tttgataaag gtaaacatgg cgattcctta tttcagcggc gagtttttac gctcaatgct
    20761 gtaacgctcg agttctttgt acggcaccac tttgctcttt ttcttacgca catcgaccac
    20821 ggtcatgcgg tcggtacagg agtagcaagg gtccaggcta ccgataatca gcggcgcatc
    20881 ggaaacggtg ttaccgcgca gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc
    20941 acggcagcgc cagcggtaca gcttctggtt gtcgccggtc atgctccagt ggatatcgtc
    21001 gccgcgcggc gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg taaagccttc
    21061 caccatcagc ggaccgcccg gcaggttatc cagaccgtag tcgatcatgt tcagcgcggt
    21121 ataaacttcg ttgatacgca ctttcagacg ggaaataacg tcgcagccct gctcgctgtg
    21181 gacttccatt ggcagcaggc cataaccgac aaacgggtga tcggcgcgag tatcgcgggc
    21241 gtgaccgctg gcgcgaacca tcgggccaac gttactgaaa tcgcgggcga tttccgggtc
    21301 cagacgacca atgccgacgg tgcgctgttc catgttcggc gtactcagca gcacatccac
    21361 cagctcctgc acttcacgac gcatctgttg cgccagctgg cgggtctgga tcatgtcatc
    21421 tttcaacaga tcgcgacgaa tcccgccgat caggttcagg ccgtaggttt tacgcgcacc
    21481 ggtgaggatc tctgccattt tcatggaggt ttcacgcacg cggaagaact gcataaagcc
    21541 ggagtcaaag ccggtaaagt ggcaggcgag accgaggttg agcagatgcg agtgcaggcg
    21601 ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt tctggcacct gaatacccat
    21661 cgcgttttct accgacgtgg tgtaggcggt gctgtgggca aagccgcaga tcccgcacac
    21721 acggtcagag aggaaggtca cttcgttgta acccatacgg gtttccgcca gtttttccat
    21781 gccgcgatga acgtagaaca gacggtagtc ggcgtcgata atgttttcgc catcgacgaa
    21841 cagacggaag tggcccggtt cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt
    21901 gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc
    21961 cggacgctga cgataatcca tgctgtcttt acgcagcgga taaagttcat ccggccagtc
    22021 atccggcagc accagacgac gttcatccgg cagaccaacc ggaatcaaac cgtacatatc
    22081 gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg acggatactc
    22141 cggtttgttg gcgtcaactt cgacgcgaac cgttacccaa cacttggtgc ccttctccat
    22201 cgacagcacg taataaacgg cgtaatgacc attcagtttg cgttcgtcgt taccaaacag
    22261 caccgacagc cagccaccct gcttgtagta gagaaactcc accacttccg gcaggtagtt
    22321 caccttcacg gtcacggtca gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc
    22381 cggaaatgcc tcattcagcg cggcgagata atgttgacct aatttttctt cagacatgct
    22441 caaactctct ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac
    22501 ccagcccagg taatgcgcgg agtaatatca agacgcagac gggccatgct gttttcgaac
    22561 agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg caatcaccag cgccagcagc
    22621 agcccaccgg cggtgaaggt ttccatctgt ccccacggga taaatacgcc gacgaacatc
    22681 tgcagcacca ccagctgttt caggctgata ccccatttca tgacgccaaa gccgctgccg
    22741 ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg cttccgccag gtcgaacggc
    22801 agtttgccca tttcgataaa ggtggcgaac gcacaggcac aaagcgccag taccagcggg
    22861 atgctctggc tcagcggcca gtgataaacg gtgtcggtga tgttgctgat gttggtggaa
    22921 ccggcaacct gtgcggcaac ccacagacca agcagcagca tcggttcgac cagcacgcca
    22981 agcatcgctt cacggctcgc gccgatagcg gtaaacgggc taccggtatc cagaccagaa
    23041 atggcaaaga agaaacgcgc gatggcaaag agatacagta aggtgattaa atcacccagt
    23101 tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg
    23161 cccaccatca catacggcgt caggcggaac acccagccgg aggcatccgg gccaacgctc
    23221 tggcggccca gcagtttgat aatgtcacga tactcctgca acacacccgg cccacgacgg
    23281 ttatgtaagc gggctcgcgc cacgcgggta ataccggaga gcagcggcgc aacggcaaat
    23341 agcaccagcg cctgaattaa cggatataaa acactcattc tcaggctcct cgtgaaacaa
    23401 taatcaccac cagtaccgcc agttcaacca gcgccatccg gcggaacagc aacgcactcc
    23461 cctcgcactg ccagcccggc accagagaca ccggattcag ccatttgcgt agtttcagca
    23521 ccggcgcaaa cgcctgtttc accggcatgg caaaaccgtg agcggtaatc accattgatt
    23581 tttcgtggtc gtagccgcac acccaggccg cgccgcggga acgcgatggc aaacgatcgc
    23641 ctttgcaaat cgccataatg atgaatggca gcagcgggca ggcaatcagc agcaatgtga
    23701 tcattggttg agaaacggtg gtgttagcag gctccagcgg cagaggtaca gcagcagaca
    23761 gcatcggcag cagccacggc gcagcaacac cgccaattac gcagcaaatc gccagtgcca
    23821 ctacgcttac cctcatcagg agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg
    23881 gcgcgccgag gaacgtgacg ccatagactt tcgccataca catcaccgcc agcgcaccgg
    23941 taattgccag acccacagcg agcagcggcc ccagcagacg ggcaacaaac gcgccgctat
    24001 tgctcagttt gaagaaggat tgatagataa cccattcccc ggcaaacccg ttcagcggcg
    24061 gcagcgcagc cattgccatt aacccgacta acatggcgat ggagataacc ggcattttct
    24121 tgccaatacc accgagtttt tcgatatcgc gatgaccggt acggaaccag acgctccccg
    24181 ccccgaggaa cagtacgctt ttgaacaggc tatggttaag cagatggtac agaccaccga
    24241 ccaggccaag agcaatcagc gccggttgtt cgagcgcgat acccgttacg ccagcgccca
    24301 gccccagcag gataatgccg atattttcca gggtgtggta agccagcagg cgctggatgt
    24361 tgtgctccat cagcgcatac agaccaccga caaacgcggt gatcatgccg agcaccagca
    24421 gcgcgatccc ccaccacagc ggtgcattac cgcccagcag tgacagggtt aaaatgccca
    24481 gcaggccaat tttcatgact accgtagaaa acaacgcggc agctggcgca gaggcgttag
    24541 catgtgcctg cggcacccag ccgtgcagcg gaataatccc ggcgagcagg ccaaagccaa
    24601 tcacaccgag cagccagata tcggaaccga gcggcagctg ttgcatacgc atatccagca
    24661 ggcgcagatc cagcgtgcca taacgctgcc acagcagcca gcaagcaatc gccagcagca
    24721 gagtgccaag acgccccagc gcaaaccaca gtttgccctc tttgctgttg ctagtgagga
    24781 acaccgcgca cagggccatg atttcggcca ttaccacgaa catgccgagg ttgctggcaa
    24841 tgacggcgca gacggcggca gccatcaaca tattgatctg caagccgttg cacttcacct
    24901 gcgcgtggcg atgccagtca atgttataga ggctgacaaa cagaccgcaa aggccgaggg
    24961 taatcagcca aatcgcgtta agcggggaga tttgcacatc gtagcttacc agcgacagcg
    25021 caccgctcac gccaaccgcg ccagtcagca cagtgaagcc cgcggctgcc gtatacaggc
    25081 taccaaccgc gccgccaatt ccggctatcc agccacttag cgtcttttga aaagaaaaga
    25141 gaaatgccag aacagcggcg gcgacaaacc acgccacgcc gctattgatc agggaaattg
    25201 cgctcattta gcctctccac tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt
    25261 gttaaaggtc agctcacgct tacgtttgct gacgcgggcg atatcggtgt tatccaccag
    25321 atgcagggct ttagtcgggc acatccgcac gcaggccgga ccttgttcat caaagctaca
    25381 aaggtcacat ttgacggcga tcgcgcgaat acctggcacc cagtcaagca atgtgctgac
    25441 acgcgccgga gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg ccggaatatc
    25501 cagcggacgg ctgccggaaa attcaattgc gccaaacggg caggcgatgc cgcacagctt
    25561 gcagcttacg cacaggcttt cattcaactg cacggcccca tcaacgcggg tgatggcgtt
    25621 aaccgggcag accaccgcgc agggtgcatc ttcacagtgg tgacagagct gcggcgcaga
    25681 ttctttttca ttcagcatca ctctcaggcg cggcattgat tgcaggccgt gctggcgatg
    25741 cgtctctgaa caggcggcct cacaagtgtg gcagccgata cagagcgtgg agtcagcaat
    25801 tacaaaacga ttcaccaggc attcctcagg tgattgtcat ttttgacgaa aacatgccga
    25861 tgaaatgtca ttttcgacac tcatcgacac gcccatcccc aaacaggcgt aacgcctgca
    25921 aaacgggcaa agcctcagct catgctgccg ggctttgtcc ctttaccagt tggcttaaat
    25981 tcaccggcac attgttttca atagcggtgt ataagctgtc gtaaacgcca gcgatttttt
    26041 cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc
    26101 cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct tttgtttcga
    26161 cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atgcgaaaat
    26221 gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc
    26281 gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct
    26341 gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca
    26401 ttgtcaggtt acccgtttaa cagagatgcc agctttaagc attttctgtg ccaactttta
    26461 atttattgtt attaaagaga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg
    26521 tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc acaattattg
    26581 cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg
    26641 cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct
    26701 ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc
    26761 tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggccg
    26821 aatgctggtg tgaaacatgc caacagtatg tgacgctact gacccagcgc gtccgccgct
    26881 gtccacagtg tcatggtgac atgctgcaga ttgtggcaga cgacggttta cagattcggc
    26941 ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc
    27001 tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc
    27061 cggcggcacg cccgaagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc
    27121 agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac gcaccgggca
    27181 tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaat aaccgtctgg
    27241 ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca
    27301 gcccaggttc cggtaaaacc accctgctga cggaaacctt aatgcgcctg aaagacagcg
    27361 ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca cgcattcgcg
    27421 ctaccggcac accagcgatt caggtgaaca ccggtaaagg ctgccatctt gacgcacaga
    27481 tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa
    27541 acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtggcgg
    27601 tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg
    27661 cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaga
    27721 agtgcctcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca
    27781 ccagcggtga agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg
    27841 cgttccaggc cagatccgca ccattgacgg caaccaggca aaagtcgacg tctgcggcat
    27901 tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt
    27961 gggccagtgg gtactggtac acgttggctt tgccatgagc gtaattaatg aagccgaagc
    28021 acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggcgcgct
    28081 gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg
    28141 atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc
    28201 cctctgcgga ttatggaagt gtgcggtggt catacccacg ctatttttaa attcggcctc
    28261 gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc ggtgtgcgta
    28321 ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc
    28381 tgtacctttg gcgacgccat gcgcgtacca gggaaacagg gatcgctgtt gcaggcaaaa
    28441 gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag
    28501 gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac catgccgacc
    28561 accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc
    28621 cagcacatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga taacggtatc
    28681 gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt
    28741 atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct tgatctactg
    28801 caaggcgtgg tcatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag
    28861 tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcgat tgccgatgtg
    28921 ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggcgtg
    28981 cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc ggcaccgcag
    29041 caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa atgtaagccg
    29101 catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg
    29161 gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg
    29221 tgaataatat ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa ttaatcaaca
    29281 gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagat caggcacgtc
    29341 ttgatctggc gcagctggta gcagaaggcg atcgtctggc gttctccacc gacagttacg
    29401 tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcactg
    29461 cgaatgacgt tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc tttatcctcg
    29521 aagaaggatt gccgatggag acactgaaag ccgtagtgac cagcatggca gaaaccgccc
    29581 gcacggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag
    29641 ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg
    29701 cacagacgct aaccgcaggc gatgtattac tggttagcgg cacgctcggc gaccacgggg
    29761 cgactatcct taacctgcgt gagcagctgg ggctggatgg cgaactggtc agcgactgcg
    29821 cggtactgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg
    29881 atgccacccg tggtggtgtg aacgcggtag ttcatgagtt cgcggcagcc tgcggttgtg
    29941 gtattgaaat ttcagaagcg gcactgccgg ttaaacctgc cgtgcgcggc gtttgcgaac
    30001 tgctgggact ggacgccctt aactttgcca acgaaggcaa actggtgatc gccgttgaac
    30061 gcaacgcggc agagcaagtg ctggcagcgt tacattccca tccactggga aaagacgcgg
    30121 cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggcgtga
    30181 aacgaaccct cgatttacca cacgccgaac cgcttccgcg tatatgctaa taaaattcta
    30241 aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac
    30301 aaaatgtcat atacaccgat gagtgatctc ggacagcaaa ggttgttcga catcactcgg
    30361 acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag
    30421 cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg
    30481 tcttattacg catcgcgtga aaaagacacc cccattaaat atgaagacga aactgttctg
    30541 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    30601 gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa atttggtcac
    30661 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    30721 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    30781 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    30841 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    30901 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    30961 attgacgata tcagcattgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    31021 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    31081 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    31141 gacgatttag ccccctatga acgcatgttg ttcgacacct ggggcaacca gattcaaacc
    31201 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    31261 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    31321 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    31381 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    31441 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    31501 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    31561 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    31621 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    31681 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    31741 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    31801 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    31861 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccgtag cgatctctat
    31921 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    31981 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    32041 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    32101 gaactggaaa acgtcattga gcgcgcggta ttgctaacac gcggtaacgt gctacagctg
    32161 tcattgccag atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc
    32221 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacggcgtg
    32281 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    32341 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    32401 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    32461 tggcaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    32521 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    32581 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    32641 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    32701 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    32761 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    32821 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    32881 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    32941 cgcagttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    33001 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    33061 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    33121 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    33181 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    33241 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    33301 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    33361 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    33421 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    33481 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    33541 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    33601 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    33661 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    33721 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    33781 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    33841 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    33901 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    33961 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    34021 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    34081 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    34141 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    34201 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    34261 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccagtgc tggtacgcga
    34321 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    34381 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    34441 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    34501 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    34561 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    34621 ggcactggaa aaacagcttt atgaagagct gttcgacctg ctgttgccgc atctggaagc
    34681 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggttaacc tggcggaacg
    34741 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    34801 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    34861 gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc ccgaacatgg gcggtaaaag
    34921 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    34981 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    35041 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    35101 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    35161 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    35221 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    35281 cgtcgccaac gtgcatctcg atgcgctgga gcacggagac accattgcct ttatgcatag
    35341 cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg gttgcagctc tggcaggcgt
    35401 gccaaaagag gtgattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    35461 gaacgccgcc gctacgcaag tggatggtac gcaaatgtct ttgctgtcag tgccagaaga
    35521 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    35581 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    35641 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    35701 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaataccca
    35761 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    35821 ccgcttacat caactctctt ttttccccga aaccgactta cttatttctg tcggcgataa
    35881 tattgatcgt gggccggaga gtcttgacgt cctgcgcctg ctaaaccagc cctggtttat
    35941 ctccgttaaa ggcaaccacg aagcaatggc gctggatgcg ttcgcgaccg gcgatggcaa
    36001 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaaaaaga
    36061 agctacagat ctgttgctga aattccatca ccttccacat attattgaaa tcactaacga
    36121 caacataaaa tatgtcatcg cacatgcaga ttatccgggg agtgaatatc tctttggtaa
    36181 agaaatagcg gagagcgaat tactctggcc tgttgatcgt gtgcagaaat cgcttaatgg
    36241 cgagttacaa caaataaacg gcgctgatta ttttatattt ggacatatga tgtttgataa
    36301 cattcagacg ttcgctaacc agatttatat tgataccggc acgccgaaaa gcgggcggct
    36361 gtcattttat aaaataaagt agtctcatgc ttcttctgtg aggcatgagt aaccaaatgt
    36421 tattgcaggc cattattcat ttttcgctac cagcaaagag agatcctgct tcgccagcgc
    36481 gcgactggca ctctccggca aaccgtcgtc tgtaataatc tgatcaaact cgcttaatgg
    36541 taacgccagc catgtcgcca cctgaccata tttcgtcgca tcacagacca aaactcgctg
    36601 gcggctggca ctggcaatcg cccgtttcac cgtgacttta tcttccgctg gcgtagaaat
    36661 cccccgcaca ctccatgacg atgcagaaat aaaagcctga tcaatcatca ggctgcgcag
    36721 catggtcgca gcggcttccc cgacacagga acggttttcc cgacacactg caccgccagt
    36781 gtgaataatt gtgcaattac tgttgtcgag caagtagttc gcaataacga aatcgtttgt
    36841 gaccacagtc agtgactcca tgtgaatcag atgctgtgct atcgctaacg tggtcgttcc
    36901 cgcatccaga tagatacaac ttcccggctg aacaagactt gccgccagct tgccaatagc
    36961 cgctttttgc gtcattgcca gcgcagtttt tacctgatga gaaggttcat gcgccacgcg
    37021 tcccggagac tggacgcctc cggacaccag cacaacggct ccctgctgct ccagtttttg
    37081 taaatcccga cgaatggtca tatgtgacac attcattctg tccgttagtt cagcaatact
    37141 gacaatgcct ttttcagcta ccatctcaag gatgatttgg cgacgctcta cgggtatcaa
    37201 cttttgctcc ttcctttgtc ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg
    37261 gcgcaactaa cgcttagttc acataaaata acacacaatg ttaatttatg tgaatcagct
    37321 caccatatcg ttatcttcca gcgcttatat tcacaacatc aaacaaaata tcacttaaat
    37381 taacaaggag agcagatgaa aacgggatct gagtttcatg tcggtatcgt tggcttaggg
    37441 tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag gtctttctac ctggggcgca
    37501 gacctgaaca gcaatgcctg cgctacgttg aaagaggcag gtgcttgcgg ggtttctgat
    37561 aacgccgcga cgtttgccga aaaactggac gctctgctgg tgctggtggt caatgcggcc
    37621 caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac aacatctgaa acccggtacg
    37681 gcagtaatgg tttcttccac tatcgctagt gctgatgcgc aagaaattgc taccgctctg
    37741 gctggattcg atctggaaat gctggatgcg ccagtttctg gtggtgcagt aaaagccgct
    37801 aacggtgaaa tgactgtcat ggcctccggt agcgatattg cctttgaacg actggcaccc
    37861 gtgctggaag ccgttgccgg aaaagtttat cgcataggtg cagaaccggg actaggttcg
    37921 accgtaaaaa ttattcacca gttgttagcg ggcgtacata ttgctgccgg agccgaagcg
    37981 atggcacttg cagcccgtgc ggggatcccg ctggatgtga tgtatgacgt cgtgaccaat
    38041 gccgccggaa attcctggat gttcgaaaac cggatgcgtc atgtggtgga tggcgattac
    38101 accccgcatt cagccgtcga tatttttgtt aaggatcttg gtctggttgc cgatacagcc
    38161 aaagccctgc acttcccgct gccattggcc tcaacagcat tgaatatgtt caccagcgcc
    38221 agtaacgcag gttacgggaa agaagacgat agcgcagtta tcaagatttt ctctggcatc
    38281 actctaccgg gagcgaaatc atgatcaaga ttggcgttat cgccgatgat tttaccggcg
    38341 cgacggatat cgccagtttt ctggtggaaa acggtctacc aacggtacaa attaacggtg
    38401 ttccaacagg taaaatgccg gaagcaatcg acgcactggt gatcagcctg aaaacgcgct
    38461 cctgtccggt ggttgaagcc acacagcaat cgctggcggc tctgagctgg ttgcaacagc
    38521 aaggttgcaa acagatctat ttcaaatact gctctacttt cgacagtacg gcgaaaggta
    38581 atatcggccc ggttaccgat gcattaatgg atgctctcga cacgccgttt acggtcttct
    38641 ctccggccct gccggtcaac ggacgtacgg tttatcaggg gtatttgttc gtaatgaatc
    38701 aactgctggc cgaatccggg atgcgccatc acccggtaaa tcccatgacc gacagctatc
    38761 ttccccgtct ggttgaagcg caatccacag ggcgctgcgg cgtcgtttcg gcacatgttt
    38821 tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg cttacagcaa gagggctacc
    38881 gctacgcggt gcttgatgcg ctgaccgaac accatctgga aattcaggga gaagccttgc
    38941 gcgatgcccc actggtaacg ggcggttctg gtctggcgat tggcctggcc cggcagtggg
    39001 cgcaagaaaa cggtaaccag gctcgcgaag cagggcatcc gctcgctggg cgcggcgtag
    39061 tgctctccgg ttcatgctct caaatgacca accgccaggt ggcacattac cgtcaaattg
    39121 caccagcccg tgaagttgat ctggcacgct gcctctcaac cgaaactctg gccgcttatg
    39181 cacacgaact ggcagagtgg gttctgggcc aggaaagtgt acttgctcca ctggtttttg
    39241 ccaccgccag cactgacgca ttggcagcaa ttcaacagca atacggtgca caaaaagcca
    39301 gtcaggcagt agaaactcta ttttctcaac tagcggcgcg gttagcagcg gaaagcgtga
    39361 cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt cacacagagc ctgggcataa
    39421 aagggtttca tattggccca accatttccc ccggcgtgcc gtgggtaaac gcactggata
    39481 agcctgtctc actcgccctt aaatctggca acttcggtga tgaagccttt ttttcacgag
    39541 cccaaagaga gtttttatca tgagcgattt cgcaaaagta gagcagtctt tgcgagagga
    39601 gatgacgcgg attgccagtt cattctttca gcgcggctat gcaaccggtt cggctggcaa
    39661 tctgtcgctg cttttacctg acgggaattt actggcgaca ccgacaggtt catgcctggg
    39721 caatctcgat ccgcagcggc tttccaaagt cgccgcggat ggcgaatggt taagtggtga
    39781 caaaccctcg aaagaggtgc tctttcatct ggcgctgtat cgcaacaatc cgcgctgtaa
    39841 agcggtggtg catttgcaca gcacatggtc gacggcgctt tcctgcctgc aagggctgga
    39901 cagcagcaac gttattcgtc cgttcacacc atacgtggtg atgcggatgg gaaatgtccc
    39961 gctggtgcct tattaccgac cgggcgataa acgcatcgca caggatctgg cggaactggc
    40021 agcagacaat caggcttttt tactggcaaa tcatggccca gtggtttgcg gtgaaagcct
    40081 gcaagaagcc gccaacaata tggaagagct ggaggaaacg gcaaagctga tttttattct
    40141 cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt gcggaattaa ggagttaatg
    40201 caatgcctcg ttttgcagct aatttatcca tgatgttcac cgaagtgcct tttattgaac
    40261 gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga atttctgttt ccctatgact
    40321 actccaccct gcaaatccaa aagcaactgg agcaaaacca tctgacactg gcgctgttca
    40381 ataccgctcc cggagatatt aatgcggggg aatggggatt atccgccctt cccggacgtg
    40441 agcacgaagc acacgcagac atcgacctgg cactggaata tgcattggcg ctcaattgtg
    40501 aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga agacgctgaa cggtaccggg
    40561 cagtatttat cgataatctc cgctacgctg ccgaccgctt tgcgctacac ggcaagcgaa
    40621 tattagttga agcactcagc cccgacgtta agcctcatta tctcttctcc agccagtatc
    40681 aggcactggc tattgttgaa gaggttgcgc gagataacgt gtttattcaa ctagatactt
    40741 ttcatgcgca aaaagtagat ggcaacctga cgcatttaat ccgcgactac gctgggaaat
    40801 atgcgcacgt acaaattgcc ggactacctg atcgacatga accggacgat ggagaaatca
    40861 actatccgtg gctgttccgc ctgttcgatg aggtgggata tcagggctgg atcggttgtg
    40921 aatataaacc tcgtggcctc accgaagaag ggcttggctg gtttgacgcc tggcgctaat
    40981 tcgttaaatc cctaattaca acgtacccat acatcccccc atctacggat ggggtaggtt
    41041 aactataact ttcagacagg gtttcccatg tccacaatta cattgttatg cattgcgtta
    41101 gctggcgtaa tcatgctgtt gctgctggtc atcaaggcaa aggtacaacc attcgttgct
    41161 ctgctcctcg tcagcctgtt agtcgcactt gcggcgggta taccggcggg cgaagtgggt
    41221 aaagtgatga tcgccgggat gggcggtgtg cttggttccg tcactattat tattggtctg
    41281 ggcgctatgc tggggcgtat gatcgaacac tctggtggtg cagagtcact ggctaattat
    41341 ttcagtcgca agttaggtga caaacgaact atcgctgcgc tgactctggc agcgttcttc
    41401 ctcggtattc ccgtcttctt tgatgtcggc tttattattc ttgcgccaat catttacggt
    41461 tttgccaagg ttgccaaaat atcgccactc aaatttggcc tgcctgtcgc tggaatcatg
    41521 ctaactgttc atgtggcggt accgccgcat ccaggccctg tcgccgcagc ggggttactc
    41581 cacgcagaca tcggctggct aaccatcatc ggtattgcga tttctattcc cgtagggatt
    41641 gttggctact ttgcagcgaa aataatcaat acgcgccaat atgcgatgtc ggtggaagta
    41701 ctggaacaga tgcaactggc tccggccagt gaggaaggcg caacaaaatt aagcgataaa
    41761 ataaatccac cgggcgtcgc gctggtcacc tcgctaattg ttattcctat cgcgattatc
    41821 atggcgggta cggtttccgc aacactgatg ccgccttcgc atcccctgct tggtacgcta
    41881 cagctgatcg gctcaccaat ggtagctcta atgattgcgc tggtgctggc attctggtta
    41941 ttggctttac gtcgcggctg gagcttacaa cacaccagcg acattatggg ctcagccctt
    42001 cccactgcgg cggtagtgat tttggttact ggtgctggag gggtatttgg caaagtgctg
    42061 gtggaatcgg gcgtcggcaa agcccttgcc aatatgctac aaatgattga cctgcctctg
    42121 ttaccagccg catttattat ttcactggct ctgcgtgcat cgcaggggtc agcaaccgta
    42181 gcaatcctga caaccggcgg gttactctca gaagcggtga tgggattgaa tccgattcag
    42241 tgcgtattgg tgacgctggc agcctgcttt ggtgggcttg gcgcgtcaca tattaatgac
    42301 tcagggttct ggattgtgac caaatatctg gggttgtcgg tagcagacgg tctgaaaacc
    42361 tggacagtgt taacgaccat tctcggtttt accggattct taattacctg gtgcgtatgg
    42421 gcggtaattt gaccttaata aaaaggtccg atgggcatcg gaccttttat ttaacgctta
    42481 ccttccacca gcagcggcgg gatgctcggc accattggcg cgtcatcaat atctttctgc
    42541 gtcatgcgga acgcttcggg ataatgttcg cggctggtac ggcgcagcgg ttcggtatcg
    42601 cgctaggtat aaaggcaatg ctggcactga tataccgtcc agacatcttt caccggcgat
    42661 ttcgccatca cttcaatctg ttcatcggca caacgtggac aaatcatctt cttctcctta
    42721 tttacgtgca gccagcatag cggtcagttt ttcagcccag gctttggttt ccggtaaatc
    42781 caccaccggt tggctgtagt gaccacggtt gtccggggcg acaggcgtag tggcgtcgat
    42841 aatcagcttg tcggtgatcc ccgcagggct tgagcctgga tcgagttcca gcacggacat
    42901 attcggcaac tgcaccaaat cccctgccgg gttcactttc gaggagagcg cccacatcac
    42961 ctgcggcagg ttgaacgggt caacgtcttc atcgaccata atcaccatct tcacgtagcc
    43021 cagaccatgc ggcgtggtca ttgcgcgcag gcccaccgcg cgggcaaagc cgccgtagcg
    43081 ttttttggtg gagataatcg ccagcaggcc atgggtgtac atggcgttta ccgcctgcac
    43141 ttcagggaac tcggctttca gctgctgata cagcggcacg caggtggctg gccccatcag
    43201 gtagtcgatt tcggtccacg gcataccgag gtacagcgat tcgaaaatcg gcctggtgcg
    43261 gtaagagact ttatcgatgc gcaccacggt catgttacgc ccgccggagt agtgcccggt
    43321 aaactcaccg aacggccctt cgatttcgcg tttacggctt tcgatgaccc cttcgaggat
    43381 cacttctgaa ccccacggca catcaaaacc ggtcaacggg gcggtggcga tcgggtacgg
    43441 gctttcacgc agcgcgcctg ccatttcgta ctcggactga tcatatttca gcggcgtggc
    43501 ccccatcagc gtgatgatcg gatcgttacc gagcgtgatc gcaatcggca gatcttcacc
    43561 gcgctcttct gctttatgca gatgcagggc gatatcgtgc atcggcaccg gttgcaggcc
    43621 gagcttacgc ttgcccttca cttccatgcg gtagatgccg acgttctgct tgccgaagtt
    43681 atccgggtcg agcggatcgc gggaaaccac gcacgctttg tcgagataga aaccgccatc
    43741 gtttaaacga aacagcggca ggatatcgaa caggttgatc tcgtcgccat caacggtgtt
    43801 ctgcgcccag gctggattgg cgcggcgctc cggggcaatc gggaagttat cccagcggcg
    43861 gataaactca tcaatctgct ttttaaccgg ggcatttggc ggcaggccga gggaaatcgc
    43921 gtggttctgc caggaaccga tggtgttcat cgccacgcgg gcatcggtaa agccgcgaat
    43981 attatcaaac cacagcgcgg gcgcgccgtc gccgatacgc ccggtggcgt tagctgctgc
    44041 tgccagatcc ggctcggcgt tcacttcttc gctgattttc agtaactggc cgtggtcatc
    44101 aagcgcctgt aaaaagctgc gtaaatcatc aaatgccatt attcattctc ctgagaaaaa
    44161 ttccgggcct gcggcaatcc ttgccagcgc ctggcgtggg gatgttcgag gccaaattga
    44221 tccagcacgc gggctaccac atggtggaca atgtcatcta ccgtttcggg atggttataa
    44281 aaggcaggca tcggcggcac catcgccacg cccatgcgtg aaagtgcgag catattttcg
    44341 agatggatgg tgctaagcgg catttcacgc ggcaccagca ccagtttgcg gccttctttg
    44401 agcacgacgt ccgccgcgcg ccctaccagg ccatcggcgt aaccagcgcg gataccggcg
    44461 agcgttttca tactgcacgg aataacgatc atgccgtcgg tacgaaaaga accggatgag
    44521 atgatcgccg cctgatccgc cgggttatgg ctgaagtctg cgagggcagc aacatcgcga
    44581 gcgctgtaag gcgtttccag ttcaatggtg gttttcgccc acttcgacat caccagatga
    44641 gtctcgacat tcggcatctc ccgcagcgct tgcagtaatg ccacaccaag aggcgcaccg
    44701 gtagcccctg tcatcccgac gatcagtttc attgcttatc ctcttaattg ttcgtatacg
    44761 aacgttatta ttaacatact ccttcatttc cacagcggca aggattgaaa agtaacaaca
    44821 tcttcttcgt cactaaaaaa ccgctatgat agcgacagag aattagcccg gagtttacat
    44881 ggcgttacga aataaagcgt tccatcagtt acgacagctt tttcagcagc acacggctcg
    44941 ctggcagcac gagttacctg acctgaccaa accacagtac gcggtgatgc gcgccattgc
    45001 tgataaacct ggtatcgaac aggtggcact gatagaagca gctgtcagca ccaaagcaac
    45061 gttagcagaa atgttggcaa gaatggaaaa tcgtggctta gtcagacgtg aacatgaccc
    45121 cgctgataag cggcgacgct ttgtctggtt gactgccgaa ggcgaaaaga tacttgctgc
    45181 ggcgataccc attggtgaca gcgtggatga ggaatttttg gggcgtttga gtggagaaga
    45241 gcaggagctg tttatgcagc tggtgcgcaa gatgatgaac gcataggggg caaatttccg
    45301 gatgcgacgc tatcgcgtct tatccagact acaaatacac cgactcacca accgaaagaa
    45361 aaaaggccag cctcgcttga gactggcctt tctgacagat gcttacttac tcgcggaaca
    45421 gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg caaacggcgc aggccttcaa
    45481 cctgaatctg gcgaacacgt tcacgggtga ggccaatttc acgacctaca tcttccagtg
    45541 ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag cacttcacgc tgtttggcgt
    45601 tcagctcgaa cagccatttg acgatgctct gcttcatatc gtcatcttgc gtggtatctt
    45661 ccggaccgtt ctctttttca tcggccagga tgtccagcaa cgctttttcg gaatcaccac
    45721 ccagcggggt gtctaccgag gtaatgcgct cgttaagacg aagcatacgg ctgacgtcat
    45781 caactggctt atccagttgc tctgcgatct cttccgcact tggttcatgg tccagcttat
    45841 gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc ctttacgatg tgaatcggca
    45901 aacgaatagt acgggtttgg ttcataatcg cccgttcaat cgtctggcga atccaccagg
    45961 ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa cttctctacc gcgcggatca
    46021 gccccaggtt gccctcttcg ataaggtcca gcaacgccag accacgattg ccataacggc
    46081 gggcaatttt taccaccaga cgcaagttac tctcgatcat ccggcggcga gaggcgacat
    46141 ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc ggccgttaac agtggtgaat
    46201 aaccaatctc accaaggtaa agctgagtcg cgtccaatac acgctgtgtg gctccctgcg
    46261 ataacagttc ctcttcggcc aaatcgttat cactgggttc ctcttcaact aaggcctttt
    46321 cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc ttcatttaaa tcatgaactt
    46381 tcagcgtatt ctgactcata aggtggctcc tacccgtgat cccttgacgg aacattcaag
    46441 caaaagcctg gttccgccga tttatcgctg cggcaaataa cgcagcgggt ttacggattt
    46501 ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg gttccggtgc tacccatggt
    46561 tgctattttt tgccccgcct tcacttcttg ttgttcccgg accagcattg tgtcgttatg
    46621 ggcgtaggca ctcaggtaat catcattatg tttgatgata atcagattac cgtagccgcg
    46681 cagcgcgtta ccggcataaa caacgcggcc atctgcggtc gcgataattg cctgtccttt
    46741 gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa gcgccaaagg tttcgatcac
    46801 tttgccctca gtcggccagc gccaggtgga gataggcgta ctggttgatg tactgctgac
    46861 agttggctcg gttgtgcttg ctgttggtac cgttacaggc gctgtgaccg tggtcgcagc
    46921 tggcttgttg ttcggcaaca ttttgttagc actctgttca cccgaagatt cagaatacgt
    46981 aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt gcaggcttga tcacaactcc
    47041 ttgctctgct gcgtcggccc gggtaatggc atttccgcca gtgattggcg taccggaagc
    47101 attacccacc tgcaaggtct gaccaacgtt cagcgcgtat ggtgcctgaa tattgttgcg
    47161 ctgagcaagg tcacggaaat cgttgccagt aatccaggcg atatagaagt gtgtcgcctt
    47221 ttttcacggt ataggtactg ccgctataac tgcctttcgg aatgttccca tactgacggt
    47281 tatagacgat gcgtccgttt tccatctgta ccggctgctg agctactggc tgcactggct
    47341 ggatttgcgg ttgttgagta gcctgaattt gtggctgctg taccggctga atttgcggtt
    47401 gctgggctgt agacgtcgtc cccattttcg gcggcggcgt aatcaacata ccagaattgg
    47461 tatttgcagg cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag
    47521 aacagcctgc cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt
    47581 ttgggcttcc cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat
    47641 taccgtgcaa ggcaccctac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg
    47701 caaaataacc aggaaaaatc caggtatttc ctcacgtttt aagccagctc acccttcact
    47761 aaaggtacaa agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc
    47821 acccgtttca aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc
    47881 agctgcgtca ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca
    47941 aacggcgcgc gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt
    48001 aaatcaagat ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc
    48061 gagcaaacat gctggacaag atgcgccagg attgccgttt gatatcccga accagtgcca
    48121 atttccagca cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata
    48181 tacggctgcg aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt
    48241 tgttcaaacg cttcatcaac gaatttttca cgcggcacgg cggcaagtgc attcagcacc
    48301 tgctcatcct gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt
    48361 accattgcgt gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat
    48421 gcgcagttaa atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa
    48481 aatcggtccc cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat
    48541 tgccgcgcgg atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg
    48601 tcacgcgaat acctttgatt tgatccaagg gtaaatccgg aacgttaata ttaagaatac
    48661 gcccggtgcg caacggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg
    48721 cagtgtcgta atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac
    48781 ggccttccat cgcggcggct accgtaccgg aataaataac atcatccccc agattcggcc
    48841 cggcgttaat tccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa
    48901 gatagacgca atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa
    48961 acgtgcgcag ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg
    49021 gggcgaccac ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg
    49081 gtgcatgtac cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata
    49141 agttccctga caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatctctacg
    49201 gtgacgtcat cccaccaatt ccagcttaat tgttgcggat acaacagcat cgctctgcgc
    49261 gcggcttcaa ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc ggcgacagct
    49321 gcttgttcga atgccagcgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg
    49381 gcggttatca tcagctcttt atcgttaacg cgacgctgta attccgccag ttcttcggtg
    49441 gttgcgacaa accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga
    49501 ttaacgtctg cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct
    49561 gccgacaacc aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag
    49621 cgctgcgccc cctgcaagtt gctaccgcca atcccaaaac gttgggcacc gaagtagttc
    49681 ggtacacctt ttacgcaaat atcgttcaga cgttgttcaa cgtcatcgcg attgctcact
    49741 tcgcgcagaa ccagggtaaa ggcgtttcct ttcagcgccc ctaaacgcag cttgcgcttg
    49801 tgccgcgcat actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt
    49861 tccttgcccg gcacgcgagc gcataaccac tgttccgtaa cagcatgttt gtctttttgc
    49921 ccagcgaagc tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca
    49981 aaacgggtat tgcagccgtt tttgaggatt ctaaccagaa tatactcacc ttcaccatca
    50041 ggctcaaagc ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg
    50101 gtgccttgcg gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct
    50161 taatgagtag cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatcccagtt
    50221 tttccgtagt agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa
    50281 taaacacgcg catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga
    50341 catcgacgtt gccaagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca
    50401 gctcgcggct atcggcacct ttaaatgccg gatcggtatc cgggaacagc ttgccgatat
    50461 cccccagcgc cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat
    50521 cagaatgcgc cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc
    50581 cttcaccgcc aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg
    50641 atggatggtt cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt
    50701 aatgttatcc gcacggcctt cgaccaactg aggatggaat ccgcaatatt ccagcgccga
    50761 ggcttcgtcg gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt catgtaacag
    50821 ctcacgaggg aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg
    50881 agcaatggca tttttgcccg gttcggcacg tttcatagta tcgcgcactg gtgcggcgag
    50941 gatccccccc gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg
    51001 caaacaagga cgagcggcgt catgcaccaa tacccactgc gcgtcgccag cggctttcag
    51061 acctgccagc acggaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg
    51121 attcgccaga ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgactcg
    51181 tttcacccgg ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg tttgattacc
    51241 gattgagaga tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg
    51301 aaccacggcg caaacatcca aatgagtggt tgccatgtta attcccgggc tgatttatcg
    51361 attgttttgc cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt
    51421 ttcgcccggc ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc
    51481 gccattgaga tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt
    51541 tgtagcttgc tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt
    51601 accgaaccac agcgaatact gtagccagac cagaatagcc agcaacagca gcgttagttt
    51661 acccatcctg ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca
    51721 tcctctgttg gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg
    51781 tatacccagg gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga
    51841 ggagacggga aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc
    51901 ctactgtcac cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca
    51961 gcgcaatccc caccactacc gcgacgggca tcagcgccag aaagaaaggc caggtgtaga
    52021 taaagaagaa cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc
    52081 aggagataaa accgaccacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa
    52141 ggctgtcgtt atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt
    52201 tgacgaaccg ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc
    52261 aacagatcta ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt
    52321 tctgccgatt caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg
    52381 gcacgcgctt tcttatataa gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta
    52441 tcgacaaaca cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg
    52501 gcgcggtgtg gcgagataaa tgcggtcagc actaccagtc cggcttcaac catcaaattc
    52561 gccacttcac cgacgcgacg gatattctct ttacgatcgg catcgctaaa accgagatcg
    52621 ctgcataatc cgtggcgaac attatcgcca tccagcagat acgtactgac gccgagttta
    52681 tgtaacgcct cctccagcgc cccggcgacc gttgatttac cggacccgga gaggccggta
    52741 aaccacagca ctacaccacg atgaccgtgg tgtagctcac gttgttgcac agtgaccgga
    52801 tggctatgcc agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc
    52861 gcgcgcccca gtgcggaaag tggcgacgaa ccagagcatt caattccagt tcgaatgcac
    52921 tgaattcaga tggcgcagca gttgcctggc taactggctc gtgcaccata ccggcaccca
    52981 cggtcacatt gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat
    53041 aacgatctaa caccagcggc tcgtcaaaag tgagatccac gaggccgatc ccattcagtg
    53101 gcaggttttc aacttcacgc tgggtaaggt tattaatatc aacctgatag cgaatgccat
    53161 caacacgcgc gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa
    53221 gcggctgttc cgccatccat accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt
    53281 cgtctgccgc cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg
    53341 tgatcgcttc tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga
    53401 cgtttgattc cacaccagag ggcagcactt tgacacgttg cccgacttcc acgcgaccgg
    53461 atgccagcgt tccggcgtaa ccacgaaaat cgagattcgg gcgattaacg tactgcaccg
    53521 ggaagcgcat tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga
    53581 gcagtgtcgg accgctgtac cacggcatac tttcactttg cgatgccacg ttgtcgcctt
    53641 ccagtgcaga gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaagg
    53701 tcaaataatc ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt
    53761 tgttgatcgc cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac
    53821 gggtttgatc gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg
    53881 atgcgccagt cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa
    53941 atttacgctt ctcggtagag aaatagcggt aggccacgtc aatggtgatg ccctgttcgc
    54001 gctcagcttg caggccgtcc accagcagag ccagatccag cttttcgccc tgggtgccgt
    54061 gacgcttact gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt
    54121 gcagcagacg accaatcaga gtacttttgc cgtcatcgac gctaccactg gtcagaaaac
    54181 gcagcaggct tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga
    54241 tttgttgtgc aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata
    54301 gaccccgcct ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc
    54361 atctcttcga tgatttccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc
    54421 cagcagccca gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg
    54481 tcgatacggt tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc
    54541 gcagcgagat atagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc
    54601 tgctcggtcc agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg
    54661 ttgtagttgt gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca
    54721 cggaaagagt aaattcgctc tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg
    54781 aaggcggcat caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata
    54841 tcggtatgtt tcgcgctgcc gtgcacgaat ggattaatcc ccatcgccac gccttccggg
    54901 tttttatgca ccagcagttc gcagccgtag gctttagcag tacgatcgcg gaactcatac
    54961 atctcgcgga atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga
    55021 taaaacgcct tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc
    55081 atcaccggat ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc
    55141 agttgccgca ggtgagtaag tcgtatttga tccataaccg ttcctttgca ataccgctat
    55201 tttcttgcca tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat
    55261 tagaatgagt agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt
    55321 aaggcaccgg atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt
    55381 catactatgg cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt
    55441 gcgccaccgt accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc
    55501 gtcacctaaa cctggcgatt ttgctaatac tcaggcacga catattgcta ctttctttcc
    55561 gggacgcatg accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt
    55621 tcagcaaatg ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc
    55681 ccgcgataat cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga
    55741 aggcaaagcg ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag
    55801 cgatgctgac gccgatgcca atctcggcgg gcttacgtta caaggaatgg atgataacgc
    55861 cgcaggttta ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc ctaccgagta
    55921 tggtattcga tttgtggcga ccagcggcga agaggaaggg aaattaggcg cagagaattt
    55981 actcaagcgg atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa
    56041 cttaattgtt ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag
    56101 gaaattaacg cgcgacaggg cgctggcaat tgcgcgtagt catggaattg ccgcaacgac
    56161 caatccgggt ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat
    56221 attcgacaaa gcgggcattg ctgtactttc ggtggaagcg actaactgga atcttgggaa
    56281 taaggatggt tatcagcaac gcgcaaaaac acctgccttc ccggcgggaa atagctggca
    56341 tgacgtaaga ctggataatc aacaacatat tgataaggca cttcctggaa gaatagaacg
    56401 tcgctgccgt gatgttatgc ggataatgct accgctggtg aaggagctgg cgaaggcgtc
    56461 ttgatgggtt tgaaaatggg agctgggagt tctaccgcag gggcggggaa ttctaagtga
    56521 tatccatcat cgcatccagt gcgcccggtt tatccccgct gacgcgggga acacaaaacc
    56581 aaacttctcc ataaattcca tagccgcggt ttatccccgc tggcgcgggg aacacgagag
    56641 tgctgacagg tgtctcgatt acctgatcgg tttatccccg ctggcgcggg gaacacgaac
    56701 gcaatatcac ggcgttctgc ggcctcggcg gtttatcccc gctggcgcgg ggaacacgtc
    56761 gggccgggta agtgaacctg tcggttttcc ggtttatccc cgctggcgcg gggaacacac
    56821 taagcataca taactgtttt taaacaaatt tattccgcat caacaatcta ccaactaaat
    56881 taaaacattt ccttattttt aaagaacgga taacttattg attatcaaca ggaagaaaag
    56941 aaaccaaacg taacccatcc aaatccaccg gaatacgtct gttttctccc caggtctgaa
    57001 attcaaaacc cgactcggta ttggtcgccc aggccatcac cacatttccg caaccagcca
    57061 gttgggtaat ttgctgccag atcatctccc gaatacgttt tgatgtatca ccaacataca
    57121 caccggcacg cacttccagt agccagattg cgagccgtcc acgtaagcgc ggcgggacat
    57181 tttctgtaac aaccacgacc atgctcatca gccgcgtcct cgatgaccgc tatcgcccaa
    57241 cgtttcaggt tcggggatgg ctggcggtaa catatccggc gcaggttgtg gcggttcaat
    57301 ttcacctgcg gcaaggactt cctcaattaa cggtattaat ttgcccgtta acttagtgct
    57361 acggaaaata tcgcgacagg ctaatctgac ttctttatca ggttctgcgg gttgcctcgc
    57421 tgctatttca aatgcctttg gcacaaccga atcaaattta atgatatcgg ctatgtcata
    57481 aacaaatgaa agcggtttgc cactatggat aaacccaata gcgggcgcat atcccgcggc
    57541 taatactgcc gcttcagata taccgtacag acatgatgta gcagcactga tgcagcgatt
    57601 cacaacatcg cctttttccc agtctttagg atcgtatttg cgaccattcc atttcacacc
    57661 atattgtttc gccagtaatg catacgtctg gcgaacgcga gatccctcaa ttccacgtaa
    57721 ctgctccact gaacggcgag ctggctgtgg ttcacgaaaa cgtaattcat acattttgcg
    57781 caccaccttc aaccgtagat cttccgttaa agccagcttt gcctggtaga gtaatttatc
    57841 tgcccgcgcc ccaccgggtt gtccggagga gtaaacgcga acgcccgctt caccgaccca
    57901 gaccagcagt gttcccaccg tggcggccag atgcaccgcc gcgtgggaaa ctctcgttcc
    57961 tggttcgagc ataatgcagg cgaccgatcc caccggaata tgcgttcgga tccctgtttt
    58021 gtcgatcagc acgaaagcgc cgtccagtac gtcgatctga ccgtactgga ggaagatcat
    58081 ggaggtgcga tcttttaatg ggatcgggct cagtggtaca aacgtcacgc ctctgctccg
    58141 ggtttgatca gcatcagacc acagccgaac gcccggcttt taccataccc ctggctcagg
    58201 cgttgcacaa ataacccagg atcggtgacc gtaagcatcc ccgtataatc cacgctactg
    58261 aactggatca gttgccggga gttttcccgc cgcagttgct gttgtcggta ggcatcaacc
    58321 gaggtatcca gcagtgtaaa accgcttctc tccccctgcg ctgccagcca gtccagcgcc
    58381 gcctgttgtt gatgcaacca gacatcactt ccttccgcct gccccctcac ctgccgtttc
    58441 gcctccatca gcagatcgtg gcgcttgccc gctttacaga ttgttggatt cgcccgcagg
    58501 ttgaaacaaa gttgttgtcc ggtacacagc tcgggcgcaa atgaccggca ttctatggtg
    58561 aacgtatcac tttccgccgg gcgttcctgc gacagtacaa aaaagcgaaa cgcgccctgg
    58621 agttcttccc gacgataaag aaattgcctt tctttgccgc cagggaagag atcccacagc
    58681 cactgatgca tcacatattc accgcgatcc accagatgca gcaactgcgc aggcgaaagc
    58741 tggccggtat gtaaggttat tcttgagagg tacatggttc ctccttgctg agccacgccc
    58801 cctgattgac ggtgcgttcc ccaaacagcc actgctgacg atttaaagga acatcccggc
    58861 ggcgtaatat tttgctcgcg accaggccgt cgtgtttccc ttcccaccag cattcatcct
    58921 gaagtttcgg gagtgagact ttcagtttat ggaaatgatc ctgatactgt tggtatgcat
    58981 tacataaggc atcacacgcg ttgccttcga gcagtaacgg cgcaagaggt aatgccagcg
    59041 gatgactttt tcgccccaga taaagcggaa aaactggatg gcgtaaaccg tcctgcaact
    59101 gttcgaggct gtaaggcgca tcggctgttg ttgccaccgc caccatccac caggcatcgg
    59161 tgtagtagtc gcgccgggag ataatcgcgc tcaaaagctc agggtcgctc aactcttcgc
    59221 gacggctgaa ataacgtgct ttacgcacct cttttggcat ctggaccgtg tgataatccc
    59281 gtgcccagcg cgggttacgg ctggcgcaaa ccaccagtga atagtggcga ttaaacgcgt
    59341 ttaagcgttc ggtatcatca cgccgaatcc ctaccccggc agccagtagc cccagcaatg
    59401 ctgagcgcga aggaagttca tgggtatgac gaacttcacc tggagcatcg cccccccagg
    59461 acgccatcgg cccatgaagc tgaaaaatca gatattggct cataagccgc cccttacgcg
    59521 cagataaagt ccagcacgtc cttcatgctt ccctgtttgt tcatcacgtc aaaacttgcg
    59581 cattcggtct tctgttcgta gaccgtattc atgttttcgc gaagcgttgt aatacgctgc
    59641 accgccacat ctaactgacg tgtgccatta atgggttcat agaaagccgc cgccagagag
    59701 cgtggttgat cggtgccttt ttctgccagc gcccaggagg cgtaggcacg gctggcaaag
    59761 ctgttctgtt tgccggttgg ggagacttta agtgcggctt ccgtaaaggc acgtatggtc
    59821 tgattagcta acgcttcgtc accgccaagg ttttcgacca gcagatcttt atcaatgcag
    59881 atataggtgt agaacagtgc agaaccgaat ccggtttcac caagatgccc ggcaccggca
    59941 tcttcagaag cctggcgcaa atcatcaacg gcggtgaaaa aatcatcttc gacaatcgtt
    60001 tcactgacac caaatgcgtg cgctacctgg caggcggctt caacattaaa ctcgggttta
    60061 ttcgccagca tacgaccaaa catggcaata tctaccgcca tacggtcttt gcgtaacaac
    60121 gcgagatctt cctcttttgg cgcgcgcttt tcttctgcaa gctgatgggc cagcgctttt
    60181 acggcgtcaa attctgccgg gctgatatgg actaactgtt cagtttcggc gttagtgagc
    60241 ggatcttttg gttttttgtc atttttagct ttcccaagat aatcagcaat ttttgccgac
    60301 cattcgatgg cttttttatc ttcaatccct ttctcaatca ggatagttgc cgcctcacgc
    60361 gcaatacgcc cactgcgaat accaatatgg cccgccagtg cctgttcaaa aagtgcagaa
    60421 gtgcgccacg cacgtttcag actttgcgag gaaacgcgca gacgcgttgc tccacccagc
    60481 accacagttt ttggcgcacc ggtatcatca cggttaaggt tggcagcagg gtaagcggtt
    60541 aacaaatgaa gctgaataaa tgttgtcatg agatagtcct ttattgattt tgttcgttat
    60601 cggcgtcgcc agcctggtaa tattccagcg cccagcggat acggataaat tcggttggcc
    60661 gctgctggcg gcggtgatga ttgagcaggt cgtcgctctc ctggcaccaa cggaagaccc
    60721 cctctgccag agagtcaaga ttgacggaac cgtttaataa tctgactgcg cggcgtaact
    60781 ggcgcagtaa ttcatccggt gtttttactg ccgacagacg agtaaaacgc ccctttgaca
    60841 ttaccgccgc cagctgcgcg gcaaaaggct gccgttcatc aatagctttt acattggcgc
    60901 taactgcggc gaccagcgcc agtgcggtaa aacgccactc ttgtgcttca attttccaca
    60961 aagtgtgggt ctgcatcagc agagaacgaa acccttctga caagcaaaca tcattaaccg
    61021 ttgtgctacg ccgcagactt gcacgattgc cgcgtttagc ctgcaactct tcgcgccatt
    61081 tacgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt
    61141 ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtgaaatatt tcttgcgcgc
    61201 ggtcataacg cgtttcaaat caacaggctc atatggattg gtgaatacac gctcgtcaaa
    61261 atcctgacgc gcgaataacc aaatttcctt ttgccatttg ccgagtaatt cgtccgcatc
    61321 ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa
    61381 gtcgatatcc acaaaactga aatcaccccg tgcacctttt ggatcggaga accacgcttc
    61441 tttcaatgca cttcgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagacgcag
    61501 tttcggtatc tggccttctt ttttcttgag cagcagcggg aaatggtgtt cgtaccaaca
    61561 gcgcgctttc atgttgtcga aatcatagcc aaatccccac aggcctactt ttgcctgttt
    61621 cagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga
    61681 tttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt
    61741 gaccgagtaa aactcgccac cctctttaag tggtatacgg taaggcgtta agggatgctg
    61801 ccacatggca taattcgcac cgtaattttt ggtcgtcatc aggctcagga gcgcgtcgct
    61861 ctgttcaccg caaatatcgc agttgccgac tgtcgtggta ttaaaatcaa tacgaatacg
    61921 gcgcggcatt ccccagtaag cctggagttt attgacctga tcatgggtta ccaccgcacc
    61981 ggccagttcg ctggttcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt
    62041 gggtagcggt aagtcggctt catcctgcgg catcacattg ggccacagtt tgcgccacaa
    62101 gggcgtttgt tgattgcctt gatattcctg taattcaatc agagtggtca tcggcccacc
    62161 gccgcgcaaa ccggtgcgat agcctttgcc gcctgatggc gcatttaact gtagggagaa
    62221 cagagctaac gcggaacaat gagggcatac gtgttcagtc acgccacgct taataaagtg
    62281 gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgacctg
    62341 aactttatct cccttgagcg cctcgaaatc ctgcataaat gaaggggaat ctgccccaaa
    62401 ctggaaagcg tgttctaagg acagcaatgc ttcccgtagc ttttcagcct ccagtccgtc
    62461 ttgccagata tcatcccaac gaccgtgatt tttgggcgcg aagctggttt gtagtagccc
    62521 cagcaaaaac tgccaggccg taccctggag atctgcccgc ggcgcagcga tatcgacaac
    62581 attttcatca gccagatcga ctggtgccag cttgcctgtc gttccgtctt taaaacgaac
    62641 aggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag
    62701 accatcccag gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag
    62761 tcctgtcgca gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccgc
    62821 aactttcgtc atcgttgact aaaatcacgt ttgccatctc cggatcttgt cgctgttcaa
    62881 tgcaccacag cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc
    62941 accagcttcg gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag
    63001 gcttcaccac accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac
    63061 gtgtcgacag tttttccggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt
    63121 tcagcacaaa ctttttagcc attgcgcgat cgcagtaata tttgcccact tgctcctgct
    63181 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa
    63241 gccgggctgc gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc
    63301 agattcgccc atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt
    63361 cgtcgccagg agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct
    63421 cgtcctttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc
    63481 ctgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcaa
    63541 tatccacgct ttgctcaatt acttgtgtac aaacaatgac ttgcgacgct ctctgtaaac
    63601 tacaatattt accaaagcgt gccagcgttt ccgtttcaat tcgctggcga tcgctaaaag
    63661 caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct
    63721 gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
    63781 gacttaccgc cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc
    63841 tgacgctacg ctcaacatct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg
    63901 tgacatgcgt cagccagggg taatcatcct tttccaggaa cggagcttct tgctggccct
    63961 ctgtaccccg cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag
    64021 aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc
    64081 acgacatgta agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc
    64141 ccagaagccg cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg
    64201 tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac
    64261 atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga
    64321 gtgattcatt aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc
    64381 gggattctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg
    64441 ccgttgccat tgttggcaga ccaaaaaaca gcccctgagc tttccccgca gctatcaacc
    64501 tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa
    64561 ataactgcgg tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
    64621 gattctcaat aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta
    64681 gaggaaagac ggttaaggcg ttttgagcct gaattaaagt tttctgccag taacctttaa
    64741 tatccattgg atgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga
    64801 gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat
    64861 catcccagaa tgcggggctc tctatgagcg gaaacagtgg tttgatttca aggagaaaat
    64921 ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg
    64981 gctgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc
    65041 gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca
    65101 accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa
    65161 tcttgagttc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt
    65221 gccagcacag caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt
    65281 gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg
    65341 ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc
    65401 gtcggttttt tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta
    65461 aaggatattt acgcatattt taaaaattat ctgtgatata tatcagccaa taaaacattt
    65521 acattatgaa taaattaatg atcttcattt gaaattcata ata
//
LOCUS       DAZUFB010000015        63138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZUFB010000015 DAZUFB010000000
VERSION     DAZUFB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.015"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..150
                     /locus_tag="ACHY3M_001118"
     CDS             16..150
                     /locus_tag="ACHY3M_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFS4316075.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            129..233
                     /gene="speFL"
                     /locus_tag="ACHY3M_001119"
     CDS             129..233
                     /gene="speFL"
                     /locus_tag="ACHY3M_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFS4316076.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            544..2742
                     /gene="speF"
                     /locus_tag="ACHY3M_001120"
     CDS             544..2742
                     /gene="speF"
                     /locus_tag="ACHY3M_001120"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFS4316077.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2739..4058
                     /gene="potE"
                     /locus_tag="ACHY3M_001121"
     CDS             2739..4058
                     /gene="potE"
                     /locus_tag="ACHY3M_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFS4316078.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYALVLPRFELKNKHG"
     gene            complement(4114..5754)
                     /gene="pgm"
                     /locus_tag="ACHY3M_001122"
     CDS             complement(4114..5754)
                     /gene="pgm"
                     /locus_tag="ACHY3M_001122"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFS4316079.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5780..6325)
                     /gene="seqA"
                     /locus_tag="ACHY3M_001123"
     CDS             complement(5780..6325)
                     /gene="seqA"
                     /locus_tag="ACHY3M_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFS4316080.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6510..7274
                     /gene="ybfF"
                     /locus_tag="ACHY3M_001124"
     CDS             6510..7274
                     /gene="ybfF"
                     /locus_tag="ACHY3M_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4316081.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7414..7707
                     /gene="ybfE"
                     /locus_tag="ACHY3M_001125"
     CDS             7414..7707
                     /gene="ybfE"
                     /locus_tag="ACHY3M_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFS4316082.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7847..8377
                     /gene="fldA"
                     /locus_tag="ACHY3M_001126"
     CDS             7847..8377
                     /gene="fldA"
                     /locus_tag="ACHY3M_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFS4316083.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8587..8673
                     /gene="uof"
                     /locus_tag="ACHY3M_001127"
     CDS             8587..8673
                     /gene="uof"
                     /locus_tag="ACHY3M_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFS4316084.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8666..9112
                     /gene="fur"
                     /locus_tag="ACHY3M_001128"
     CDS             8666..9112
                     /gene="fur"
                     /locus_tag="ACHY3M_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFS4316085.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9196..9522)
                     /gene="chiQ"
                     /locus_tag="ACHY3M_001129"
     CDS             complement(9196..9522)
                     /gene="chiQ"
                     /locus_tag="ACHY3M_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFS4316086.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9572..10978)
                     /gene="chiP"
                     /locus_tag="ACHY3M_001130"
     CDS             complement(9572..10978)
                     /gene="chiP"
                     /locus_tag="ACHY3M_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFS4316087.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11425..13089)
                     /gene="glnS"
                     /locus_tag="ACHY3M_001131"
     CDS             complement(11425..13089)
                     /gene="glnS"
                     /locus_tag="ACHY3M_001131"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFS4316088.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13292..15238)
                     /gene="nagE"
                     /locus_tag="ACHY3M_001132"
     CDS             complement(13292..15238)
                     /gene="nagE"
                     /locus_tag="ACHY3M_001132"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFS4316089.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15571..16371
                     /gene="nagB"
                     /locus_tag="ACHY3M_001133"
     CDS             15571..16371
                     /gene="nagB"
                     /locus_tag="ACHY3M_001133"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4316090.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16431..17579
                     /gene="nagA"
                     /locus_tag="ACHY3M_001134"
     CDS             16431..17579
                     /gene="nagA"
                     /locus_tag="ACHY3M_001134"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4316091.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17588..18808
                     /gene="nagC"
                     /locus_tag="ACHY3M_001135"
                     /gene_synonym="nagR"
     CDS             17588..18808
                     /gene="nagC"
                     /locus_tag="ACHY3M_001135"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFS4316092.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18856..19608
                     /gene="nagD"
                     /locus_tag="ACHY3M_001136"
     CDS             18856..19608
                     /gene="nagD"
                     /locus_tag="ACHY3M_001136"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFS4316093.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19865..21529
                     /gene="asnB"
                     /locus_tag="ACHY3M_001137"
     CDS             19865..21529
                     /gene="asnB"
                     /locus_tag="ACHY3M_001137"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFS4316094.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALEN
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21910..21986
                     /locus_tag="ACHY3M_001138"
     tRNA            21910..21986
                     /locus_tag="ACHY3M_001138"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21944..21946,aa:Met,seq:cat)
     gene            21995..22079
                     /locus_tag="ACHY3M_001139"
     tRNA            21995..22079
                     /locus_tag="ACHY3M_001139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22029..22031,aa:Leu,seq:tag)
     gene            22103..22177
                     /locus_tag="ACHY3M_001140"
     tRNA            22103..22177
                     /locus_tag="ACHY3M_001140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22135..22137,aa:Gln,seq:ttg)
     gene            22193..22269
                     /locus_tag="ACHY3M_001141"
     tRNA            22193..22269
                     /locus_tag="ACHY3M_001141"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22227..22229,aa:Met,seq:cat)
     gene            22318..22392
                     /locus_tag="ACHY3M_001142"
     tRNA            22318..22392
                     /locus_tag="ACHY3M_001142"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22350..22352,aa:Gln,seq:ctg)
     gene            complement(22546..23721)
                     /gene="ubiF"
                     /locus_tag="ACHY3M_001143"
     CDS             complement(22546..23721)
                     /gene="ubiF"
                     /locus_tag="ACHY3M_001143"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFS4316095.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23867..25291
                     /gene="miaB"
                     /locus_tag="ACHY3M_001144"
     CDS             23867..25291
                     /gene="miaB"
                     /locus_tag="ACHY3M_001144"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFS4316096.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25444..26484
                     /gene="ybeZ"
                     /locus_tag="ACHY3M_001145"
     CDS             25444..26484
                     /gene="ybeZ"
                     /locus_tag="ACHY3M_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4316097.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PVYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26481..26948
                     /gene="ybeY"
                     /locus_tag="ACHY3M_001146"
     CDS             26481..26948
                     /gene="ybeY"
                     /locus_tag="ACHY3M_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4316098.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27038..27916
                     /gene="corC"
                     /locus_tag="ACHY3M_001147"
     CDS             27038..27916
                     /gene="corC"
                     /locus_tag="ACHY3M_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFS4316099.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27941..29479
                     /gene="lnt"
                     /locus_tag="ACHY3M_001148"
     CDS             27941..29479
                     /gene="lnt"
                     /locus_tag="ACHY3M_001148"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFS4316100.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29557..31434)
                     /locus_tag="ACHY3M_001149"
     CDS             complement(29557..31434)
                     /locus_tag="ACHY3M_001149"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4316101.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASEAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            31889..32797
                     /gene="gltI"
                     /locus_tag="ACHY3M_001150"
     CDS             31889..32797
                     /gene="gltI"
                     /locus_tag="ACHY3M_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFS4316102.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKSGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            32967..33707
                     /gene="gltJ"
                     /locus_tag="ACHY3M_001151"
     CDS             32967..33707
                     /gene="gltJ"
                     /locus_tag="ACHY3M_001151"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFS4316103.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            33707..34381
                     /gene="gltK"
                     /locus_tag="ACHY3M_001152"
     CDS             33707..34381
                     /gene="gltK"
                     /locus_tag="ACHY3M_001152"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFS4316104.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34381..35106
                     /gene="gltL"
                     /locus_tag="ACHY3M_001153"
     CDS             34381..35106
                     /gene="gltL"
                     /locus_tag="ACHY3M_001153"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFS4316105.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35224..36159
                     /gene="rihA"
                     /locus_tag="ACHY3M_001154"
     CDS             35224..36159
                     /gene="rihA"
                     /locus_tag="ACHY3M_001154"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFS4316106.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36243..37913
                     /gene="hscC"
                     /locus_tag="ACHY3M_001155"
     CDS             36243..37913
                     /gene="hscC"
                     /locus_tag="ACHY3M_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFS4316107.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(37973..39424)
                     /gene="djlC"
                     /locus_tag="ACHY3M_001156"
     CDS             complement(37973..39424)
                     /gene="djlC"
                     /locus_tag="ACHY3M_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFS4316108.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39421..40128)
                     /gene="ybeU"
                     /locus_tag="ACHY3M_001157"
     CDS             complement(39421..40128)
                     /gene="ybeU"
                     /locus_tag="ACHY3M_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4316109.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            40229..40783
                     /gene="ybeT"
                     /locus_tag="ACHY3M_001158"
     CDS             40229..40783
                     /gene="ybeT"
                     /locus_tag="ACHY3M_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4316110.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(40793..42220)
                     /gene="djlB"
                     /locus_tag="ACHY3M_001159"
     CDS             complement(40793..42220)
                     /gene="djlB"
                     /locus_tag="ACHY3M_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFS4316111.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(42217..42924)
                     /gene="ybeR"
                     /locus_tag="ACHY3M_001160"
     CDS             complement(42217..42924)
                     /gene="ybeR"
                     /locus_tag="ACHY3M_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4316112.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            43088..44065
                     /gene="ybeQ"
                     /locus_tag="ACHY3M_001161"
     CDS             43088..44065
                     /gene="ybeQ"
                     /locus_tag="ACHY3M_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFS4316113.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(44135..44617)
                     /gene="ybeL"
                     /locus_tag="ACHY3M_001162"
     CDS             complement(44135..44617)
                     /gene="ybeL"
                     /locus_tag="ACHY3M_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFS4316114.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            44852..47434
                     /gene="leuS"
                     /locus_tag="ACHY3M_001163"
     CDS             44852..47434
                     /gene="leuS"
                     /locus_tag="ACHY3M_001163"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4316115.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            47449..48030
                     /gene="lptE"
                     /locus_tag="ACHY3M_001164"
     CDS             47449..48030
                     /gene="lptE"
                     /locus_tag="ACHY3M_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFS4316116.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDKEQTSTTTDTPATPARVSTTLGN"
     gene            48030..49061
                     /gene="holA"
                     /locus_tag="ACHY3M_001165"
     CDS             48030..49061
                     /gene="holA"
                     /locus_tag="ACHY3M_001165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4316117.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVICQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            49063..49704
                     /gene="nadD"
                     /locus_tag="ACHY3M_001166"
     CDS             49063..49704
                     /gene="nadD"
                     /locus_tag="ACHY3M_001166"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4316118.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            49728..50339
                     /gene="cobC"
                     /locus_tag="ACHY3M_001167"
     CDS             49728..50339
                     /gene="cobC"
                     /locus_tag="ACHY3M_001167"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFS4316119.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPIPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            50599..50916
                     /gene="rsfS"
                     /locus_tag="ACHY3M_001168"
     CDS             50599..50916
                     /gene="rsfS"
                     /locus_tag="ACHY3M_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4316120.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            50920..51387
                     /gene="rlmH"
                     /locus_tag="ACHY3M_001169"
     CDS             50920..51387
                     /gene="rlmH"
                     /locus_tag="ACHY3M_001169"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4316121.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            51418..53319
                     /gene="mrdA"
                     /locus_tag="ACHY3M_001170"
     CDS             51418..53319
                     /gene="mrdA"
                     /locus_tag="ACHY3M_001170"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFS4316122.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            53322..54434
                     /gene="mrdB"
                     /locus_tag="ACHY3M_001171"
                     /gene_synonym="rodA"
     CDS             53322..54434
                     /gene="mrdB"
                     /locus_tag="ACHY3M_001171"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFS4316123.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            54445..55533
                     /gene="rlpA"
                     /locus_tag="ACHY3M_001172"
     CDS             54445..55533
                     /gene="rlpA"
                     /locus_tag="ACHY3M_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFS4316124.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            55673..56884
                     /gene="dacA"
                     /locus_tag="ACHY3M_001173"
     CDS             55673..56884
                     /gene="dacA"
                     /locus_tag="ACHY3M_001173"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFS4316125.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            56995..57258
                     /gene="ybeD"
                     /locus_tag="ACHY3M_001174"
     CDS             56995..57258
                     /gene="ybeD"
                     /locus_tag="ACHY3M_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFS4316126.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            57359..58000
                     /gene="lipB"
                     /locus_tag="ACHY3M_001175"
     CDS             57359..58000
                     /gene="lipB"
                     /locus_tag="ACHY3M_001175"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFS4316127.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            58259..59212
                     /gene="ybeF"
                     /locus_tag="ACHY3M_001176"
     CDS             58259..59212
                     /gene="ybeF"
                     /locus_tag="ACHY3M_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFS4316128.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            59421..60386
                     /gene="lipA"
                     /locus_tag="ACHY3M_001177"
     CDS             59421..60386
                     /gene="lipA"
                     /locus_tag="ACHY3M_001177"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFS4316129.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(60487..60690)
                     /gene="tatE"
                     /locus_tag="ACHY3M_001178"
     CDS             complement(60487..60690)
                     /gene="tatE"
                     /locus_tag="ACHY3M_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFS4316130.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(60819..61607)
                     /locus_tag="ACHY3M_001179"
     CDS             complement(60819..61607)
                     /locus_tag="ACHY3M_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFS4316131.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            61700..62083
                     /gene="crcB"
                     /locus_tag="ACHY3M_001180"
     CDS             61700..62083
                     /gene="crcB"
                     /locus_tag="ACHY3M_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFS4316132.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(62137..62346)
                     /gene="cspE"
                     /locus_tag="ACHY3M_001181"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(62137..62346)
                     /gene="cspE"
                     /locus_tag="ACHY3M_001181"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS4316133.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(62521..63081)
                     /gene="pagP"
                     /locus_tag="ACHY3M_001182"
     CDS             complement(62521..63081)
                     /gene="pagP"
                     /locus_tag="ACHY3M_001182"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFS4316134.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFRDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        15758 a        16014 c        15788 g        15578 t
ORIGIN      
        1 cgctattcat aattaatgat ttattatttg gggtatgatc gttttttgtt gatcttcttc
       61 acagattata gccatttcat ggatagaata actctacctt caactgacac agcaagaggt
      121 aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca
      181 tgaagtttgc ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg
      241 actaaagggc accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg
      301 tattcctcgg tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc
      361 acttgcaact tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat
      421 ctgcaaaaag caaactactg atttatttat cagcggtgga gctttgcttt ttttccggcg
      481 tgatcgattt ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaac
      541 aaaatgtcag aattaaaaat tgccgttagt cgttcttgcc cggattgttt ttccactcat
      601 cgtgcatgcg tgaatataga cgaaagtaat tatattgacg ttgccgccat tattttatca
      661 gtcagtgatg ttgaacgtgg aaaactcgat gaaatagacg ctactggcta tgacattcct
      721 gtttttattg caacggaaaa tgaagaacgt gtacccgcag aatatctgcc acgtatttct
      781 ggtgtcttcg aacattgcga atcacgtaaa gagttttatg gacgtcagtt agaaaccgct
      841 gccagccatt atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtt
      901 aatcagggca atagtgcttt tgactgccct ggtcatcagg ggggtgagtt tttccgtcgc
      961 catccggcag gcaatcagtt cgtggaatac tttggcgaaa tgctcttccg ctctgacttg
     1021 tgtaatgctg acgtagcaat gggcgatctg ctgattcatg aaggtgcgcc atgtattgca
     1081 caacagcatg cggctaaagt ctttaatgcc gataaaacct atttcgtttt aaacggtact
     1141 tcatcttcta ataaagtggt cttaaacgca ttgctgacgc cgggcgatct ggtgttgttt
     1201 gaccgcaata accataaatc taaccaccac ggtgcgttgc tacaggctgg cgcgacaccg
     1261 gtttatctgg aaaccgcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt
     1321 tttgaagaaa gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag
     1381 gctcgtcctt tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac
     1441 gcccgccagg tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca
     1501 tgggttggct atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat
     1561 cttaatgaga acgatccggg tattctggtt acgcaatccg tacataaaca gcaggctggt
     1621 ttttctcaaa cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat
     1681 gtaccgcaca aacgcatgaa caacgccttt atgatgcatg cctccaccag cccgttctat
     1741 ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat
     1801 atgtggatgg attgtgtggt aaatggcatt aatgcccgca agctgatcct cgataactgt
     1861 cagcatattc gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa
     1921 acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg taccaggtga gcactggcac
     1981 tcttttgaag gctatgcaga gaatcaatac tttgtcgatc catgcaaact gttgctgaca
     2041 accccaggta ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt
     2101 cttgctaact tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taactccatc
     2161 ctcttcctgc tgactccggc agaagatatg gccaagcttc agcaacttgt tgccctgctg
     2221 gtacgcttcg aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc
     2281 tacaaacagc atgaagagcg ctacgccggt tataccctgc gtcagttgtg tcaagaaatg
     2341 catgatttgt atgcccgtca caacgtgaaa caactgcaaa aagagatgtt ccgtaaggaa
     2401 cacttcccac gcgtaagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa
     2461 gtggagctgg ttcgtctgcc ggatgcagaa ggccgtatcg ctgccgaagg tgcgcttcct
     2521 tatcctccgg gtgtgctgtg tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt
     2581 tacttcagcg ctctggaaga agggatcaac ctgctgccag gttttgcacc ggagctgcag
     2641 ggtgtctata tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct
     2701 cgtgatgcgc aaagcaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca
     2761 aaatgggcgt cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca
     2821 tcatgctgcc gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga
     2881 cagccgtagg ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta
     2941 aatcaggcgg tatgggcggt tatgcggagt atgctttcgg taaatccggt aactttatgg
     3001 cgaactatac ctacggcgtc tcgctgctga ttgctaacgt cgcgattgct atttcggcgg
     3061 ttggttacgg caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga
     3121 ccatcggggt gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc
     3181 aaatcagtag cattaccgtg tggggggtca ttattccggt ggtaggcctg tgcattatcg
     3241 gctggttctg gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt
     3301 tcttcagtgc agtaggttct tccatcgcta tgacgctgtg ggcttttctc ggtctggagt
     3361 ctgcgtgtgc gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac
     3421 tcggcggtac gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga
     3481 ttgtgcctaa tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga
     3541 tgttcacacc agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt
     3601 cgctgcttgg ctggcagttc accattgccc aggtgtttaa atcttcatct gatgaaggct
     3661 acttccctaa aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga
     3721 ccattgtgat tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc
     3781 agttcaacgt gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa
     3841 tggcggcatt agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg
     3901 caaactttgt tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg
     3961 aagaagccat gctgtacggt tccatcgtga ccttccttgg ttggacactg tatgctctgg
     4021 tattaccacg ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg
     4081 atcttgcgac cgcccttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac
     4141 aatctcaacc gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca
     4201 gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt
     4261 catcactttc agaccgccga tagaagcacc attacccggt gccgccgtca gacgtgcggt
     4321 gatcgggtca cctgccaggg tgctggcgct caccatttcc ggagacagct tagacaacgc
     4381 cgctttttgt gcggaagtcg cagcggcctg caaacggttg tagctcggcg caccaaagcg
     4441 ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc
     4501 cgccagcaga cacataatga tgccgtcttt atcggtggac cacggcgtgc cgtcgaaacg
     4561 caggaaggaa gccccggcgc tctcttcgcc gccaaagccg aaactgccgt cgaacagacc
     4621 atcaacaaac catttgaatc ctaccgggac ttctaccagt ttacggccca aggcgttgac
     4681 cacacggtcg atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca
     4741 ctgcggacga tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat
     4801 caaacctgcc ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag
     4861 atcgaactta tcacgcagtg ccagcaggcc cgccatcgca cactcggagg agcagtccat
     4921 acggatcgcg ccgtctttat caaggtgcat aaagcggaag gtttgatcga cctgatcgtt
     4981 aacgatggtc aggttgaggt tgtaatactc gccaatacgc ttccagtatt cgataccgga
     5041 accgcccagc ggatcaacgc caagcgtcag gcccgctttc tgaatcgccg ccatatcaac
     5101 gatatcggcc agaccttcca caaacggctg caccagatcc tgctctttca catgaccgga
     5161 tgccatcgct tcgtcgagag agatacgctt cacgcctttc aggccatcgg ccatcagtgc
     5221 gttggccctg tcttccacca ctttggtgac gttggtatca gccgggccac catttggcgg
     5281 gttgtacttg ataccaccat cttccggcgg gttatgggac ggtgtaatca cgataccgtc
     5341 tgccagcggg ccaccttttt tattgtgaac caggatggca ttggaaacgg caggcgtcgg
     5401 agtgaatcca ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag
     5461 tacggaaatg aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc
     5521 agtgatgcca tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg
     5581 ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc
     5641 gtgctccgca ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac
     5701 gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt
     5761 gtcctttgtc tgcaacgttt tagatagttc cgcgaacctt ctcaatcaat tccgccggga
     5821 attgcatcga ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg
     5881 tgatcaccca atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca
     5941 gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg
     6001 cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat
     6061 tgaccgctcg cttttgctct gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa
     6121 ccttgtcttt aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa
     6181 cctctttcgt caccggagca gcaggctgtg atgaggcgga aaatttcaac atacgccgta
     6241 aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt
     6301 catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct gaatacaagt
     6361 cgccaggagt atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa
     6421 actcggaata aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa
     6481 cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac
     6541 aaaaccagca caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca
     6601 accttggcgt actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc
     6661 gtaaccacgg tctttcaccg agagatccgg taatgaatta cccggcgatg gcgcaggatc
     6721 ttgttgatac tctggatgca cagcagatcg acaaagcaac atttatcggt cactccatgg
     6781 gcggtaaagc ggtaatggca cttactgcac tggcccccga tcgcatcgat aaactggtgg
     6841 cgatcgatat cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcggcta
     6901 tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcgcc
     6961 agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt
     7021 ggcgctttaa cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa
     7081 tcccggcatg ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg
     7141 agcagtaccg tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag
     7201 gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct
     7261 atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg
     7321 gcgttggcgt gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc
     7381 tggctgattg tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg
     7441 acattagatc tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg
     7501 cgcgatgaac agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc
     7561 cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg
     7621 gagtcgcgca atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg
     7681 gcgctgcgta gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc
     7741 tgctcgtttg ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc
     7801 aattttccac ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg
     7861 catctttttc ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca
     7921 gcttggtaaa gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga
     7981 agcttatgac attctgctgc tgggcatccc aacctggtat tacggcgaag cgcagtgtga
     8041 ctgggatgac ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct
     8101 gtttggttgt ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat
     8161 ccgcgacatc attgaaccgc gcggtgcaac catcgttggt cactggccaa ctgcgggcta
     8221 tcatttcgaa gcatcaaaag gtctggcaga tgacgaccac tttgtcggtc tggctatcga
     8281 cgaagaccgt cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc
     8341 tgaagagttg catctcgacg aaattcttaa tgcctgatgt gatgcggcgt agactcatgt
     8401 ctacgccgta ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc
     8461 tgttgtacct gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt
     8521 gccacgtttt tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat
     8581 tctataatga tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac
     8641 cgcttagtaa caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg
     8701 cctgaaagta acgcttccac gtttaaaaat cctggaagtt cttcaggagc cggacaacca
     8761 tcacgtcagt gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct
     8821 ggctacggta tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa
     8881 ttttgaaggc ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat
     8941 ttgcctcgac tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg
     9001 tgaaattgcc gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca
     9061 ctgtgccgaa ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat aagccagcct
     9121 gaacgagaaa agccaacctg cgggttggct tttttataca agggaaataa gaaaagcgag
     9181 agttacagct ctcacttatt tgttattact gcgtatttcc tgccagacct tatcacaatc
     9241 ggctttcacc gcctgatcat ttccggtttg cgttgcacgc agacactgct gataatccag
     9301 tacgcgtaca ctttcctggt cggcaaactg ctgatgttgt ttctctttct tcagcacgtt
     9361 taacacgctc tggcaggctt caattttttc tggcgaacct tgtgcggtgt tgatacaggc
     9421 gctgtatgcc tctttcagac gactgtcttc ctgtggcgcg gttgattgcg cacaagctac
     9481 cagccccgat gccattatgg cgatgagaat cagttttttc atagcaaatt cctcaaacgt
     9541 gcggcaggca caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt gcgattacca
     9601 taaatttcac gtcacgctca tcctggaaga tgttaccgta accgccgccc cagcttggga
     9661 tgtcagagtg gttgtcgtat tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac
     9721 cgtcctgaat ggtgtagacc gcatccaggc tgtaggcaga ctcttcaata gtccggtttt
     9781 tgtcgtagta cgcatccgga ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa
     9841 cgtaggaagc gccgatggcg aagcctggaa gattccagtt tttcaggtca tacatcgcac
     9901 cgaagaagac cgctttttcg ccgttggcgt tgaagtcgga acggttatcc caccagatat
     9961 ccaggcggcc gttggaggaa gcgtaggttg gagtcatacg ttgcaggaag tatccctgct
    10021 gaccgtcagc cttaacccag gtgccttcga ggcgcaaatc cactacgtca gccgcccggt
    10081 aaccaaaggt caacgcttgc agccaggcgg tgccgtcata aaggtcgttg acgctgcgat
    10141 cgtcaacttt atcgcgcgta ccatagaact ggtagctggt ggttaacggg ctaccggcga
    10201 tatcaaattt gtagctggct ttggcaaaat attgatcgat atacccttcc gcctgaccaa
    10261 atgccgcttc cagtacgaag ttatttttga agtcgtattt cgcaccgatg gagtgcaggt
    10321 aatcaacttt ggtggtttta tcgttctgat aaaactcatc catttccaga tgccacggcg
    10381 ctttgtattc gttggtccac atgtaggaga aactcaaggc accagcatcg ccgtaatcaa
    10441 aattcgcccc ggcttccgcc ccctgataag tacctggcat aaagctccag tgcggcgcta
    10501 acagcgtttg accagttggc tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa
    10561 atttggccgc agctttatac aggcttatac cgcttttgtc gccggaccag tcttcgtcat
    10621 aggctttatt actttttgaa aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg
    10681 ccatttcaat cgccgtaaac gcagcaatat caaggccgaa catatcagca gcataaccgg
    10741 actgaaaatc gaggttggca ttccaggtgg agtgagaaag gttggttttg tatttgtcgc
    10801 cgtcggtaac atctttacgg tcgcgttcac gctgccagta atatataccg ccggttaagg
    10861 ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat tgccataaag cccgacattg
    10921 ctgtaacacc ggcgatagcc agcgccagcg tactacgttt gccactaaac gtacgcatgg
    10981 gttaatcctc tttgacgtat aaattgctgc accaaaggtg aacagcgaaa aaaataaaaa
    11041 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
    11101 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    11161 tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc
    11221 gtgaatttcc agcaagatga aagcggttac attttattta attatggttt gtgattttaa
    11281 atttatttta tggataaatt gcgaagcgga attaattcgc gaagaaaagc agaaaaaacg
    11341 ccgcagagtg cggcgtttgg gattgtttgc tatttcttgc cggatgcgac gtaaatgcct
    11401 cacccggcct ggtagaactt aaaattactc gcctactttc gcccaggtat cacgcagccc
    11461 aacggtgcgg ttaaataccg gtttttccgc tgtggaatgg cggctgtcga ggcagaagta
    11521 accttcacgc tcaaactgga atgctttacc cgcaaccgca tctttcagcg acggttcagc
    11581 aaagccctgt ttgatcacca gcgattccgg gttaatcacc gacaggaaat catccgcagc
    11641 accagggttc ggcacgctga acagacggtc gtacagacgg atttcaaccg gcagcgcatg
    11701 tgccgcgctc acccagtgaa taacaccttt gactttacga ccatctgccg gatctttgct
    11761 taaggtgtcg gcgtcataag tacagaagat ggtggtgata ttgccttcgg catctttctc
    11821 cacgcgttca gccttaatga cgtaagcatt acgcagacgc acttctttac ccagcaccag
    11881 acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg cgatcgatcc aaatctcacc
    11941 gctaaacggc acctgacgac tccccatttc cggtttgttc ggatggttcg gcatggtgac
    12001 catttcgcct tcgccctgat agttttcgat aaccagtttc accggatcga taaccgccat
    12061 tgcgcgcggc gcattttcgt tgagatcttc acggatgcag gattccagcg atgccatctc
    12121 aatggtgttg tcctgcttgg tcacgccgat gcgtttgcag aactcacgaa tagaagcagc
    12181 agtgtaacca cgacgacgca gaccggaaat ggtcggcata cgcgggtcat cccagccttc
    12241 aacgtgcttg tcggtcacca gcaggttcag cttacgcttg gacatcacag tgtattccag
    12301 attcaggcgc gagaattcgt actggcgcgg gtgaacagga atggtgatgt tgtccagcac
    12361 ccagtcgtac aggcgacggt tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc
    12421 ttccagggca tcgctgatgc agtgggtgaa gtcgtacatc gggtagatgc accacttgtt
    12481 gccagtctgg tggtgttcag caaatttaat acggtacagc accggatcgc gcatcacgat
    12541 aaacggtgaa gccatgtcga ttttcgcacg caggcaggct ttaccttctt caaaaccacc
    12601 ggcacgcatt ttttcgaaca gcgccaggtt ctcttcaacg ctgcggtcgc ggtacgggct
    12661 gtttttaccc ggttgcgtca gggtgccgcg gtattcgcgg atctgttccg gcgtcagttc
    12721 atcaacgtac gccaggcctt tattgatcag ttcgatcgca taggcgtgga gctgatcaaa
    12781 ataatcggag gagtaacgga cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt
    12841 tttgatcgac tcaacatact cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag
    12901 gttgcactgg cctttatagt cctgggcgat cccgaagttc aggcagatag atttcgcatg
    12961 gccaatatgc agatagccat tcggctccgg cgggaaacgg gtgtgtactg tggtgtgctt
    13021 accactggcc agatcttcat cgatgatctg acggataaag ttagtcgggc gggcttctgc
    13081 ctcactcatc gtggattcct caaagcgtaa acaacgtata acggcgtatg atcttataag
    13141 cgggacaggc tgacaactgt tagtttttta aaattgataa tctaacaagg attatgggga
    13201 tgattgatga aaaaaaaagc ggcagagaag atctccaccg cttgtcattg ttggatgcga
    13261 cgctcaagcg tcgcatcagg cataaagcag attacttttt gatttcatac agcggtgttt
    13321 gacccgccac aacatggccc tgagctttaa tgatcaagcc actgaaatcg tcgatattgc
    13381 tgcaaaccac cgggctaatc atcgagcggg cgttagcgtt caggtaatcc agatccattt
    13441 ccagaatcgg ttgccctgcg cttacctgcg caccctcttc caccagacgt ttaaagcctt
    13501 taccttccag cgctacggtg tcgataccca tatggacgac gatctccgcg cctttttcgg
    13561 tttccaggca gaacgcgtgg ttggtgttga agattttcac gattgtccct gcggctggtg
    13621 atacgacgat tttatctgtc ggtttcaccg ccacaccgtc acccaccgct ttgctggcga
    13681 atgcttcgtc aggaacctga tccagtgcca cgacatcacc ggtaatcggc gataccagct
    13741 ccgcgataga taccgcgttt ggtacagcct gcggttttgc tacaggcgcg gcagttgccg
    13801 gagttgcttc agctgacgca gcggctaccg gaccacgggc aacgactttc ttcatcgcat
    13861 cgccgatgga ttctgctttc gcgccaacaa tcacctgaat agtctgtttg ttcagtttca
    13921 ctacaccaga agcacccaga cgtttacaca tcgtatcgtt gacgcgggca gagtcagcca
    13981 cggtcaggcg cagacgggtg atacaggcgt caattgcttt caggttgtca gtgccgccaa
    14041 ccgcagcaat atagttggtc gccagttgat tgaggccttc ttcagtgttg ctgttggctt
    14101 cttcagtaac gatctcgtct tctttatctt cacgacccgg cgttttcagg ttgaacatgc
    14161 ggataaccaa actgaacacc acgaagtaga tagcgaagaa gacaacaccc ataaccagca
    14221 gcatccagac gttctggctg gcggccggca ggttatacat caacgcgtag tcgatagccc
    14281 ccgctgagaa agagaagcca gcatggatac ccagcagtgt tgccacaaac aggctgatac
    14341 cggtcagcag tgcgtgcagg aggtacagca gcggtgcaag gaacatgaac aggaattcca
    14401 gcggctcagt cacaccggtc aggaacgcag taacagcaac agaaagcagc atcccgccaa
    14461 ccatcggacg acgctctttc ggtgctgcga agtacatcgc cagcgccgca cccggcagac
    14521 cgaacatcat aatcgggaag aagccggaca tgaacatccc cgcggtgccg tcaccggcat
    14581 agaagcggtt gatatcaccg tggaaaaccg tacccgccgc gttggtgaat tcaccaatct
    14641 ggaaccaggc gatggtgttc agcacctgat gcagaccggt tgggatcagc agacggttga
    14701 tgaaaccaaa gataccggaa cccagcgcgc ccgcagaaac gatccactcg cctcctgcat
    14761 ggatagcgtg ctgtaccggc ggccagacgt aaccaaaaat ggccgccagc accaggcaga
    14821 agaagccggt ggcaatcggc acaaagcgtt tgccgccgaa gaaactcagg aagtccggca
    14881 gtttaatatc ggaccaacgg ttataggctg cgccaccaac cagaccggta atgatacccg
    14941 ccagtacacc catgttaatt tctgggttga tggtcaccat cgctttggtt aacacaaagt
    15001 aacctaccgc acccgccagc gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca
    15061 caccgatggc gaagattaat gcgaggttat caaaaatcgc accgcccgcc tgggcaataa
    15121 acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa cagtgccgcc accggcagca
    15181 ccgcgatagg gagctgtaac gccctaccga gtcgctggaa aaaacctaaa atattcatct
    15241 tattccccct acgagaaccc tatttggctc gtttaaagcc gtatttttat tttgctgcaa
    15301 attgtactgc cgatgttctg taatcagatt gttagatcat ctgctacaga gtgtgtgaaa
    15361 atttaattcg tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac caaatatcgt
    15421 tatcatcact cccttttact ggctaaagca gaaaacttat tttatcattc aaaaaatcag
    15481 gtcggattga cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct aatccgccaa
    15541 cggcttacat tttacttatt gaggtgaata atgagactga tccccctgac taccgctgaa
    15601 caggtcggca aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact
    15661 gccgatcgtc cgtttgtact gggcctgccg actggcggca cgccgatgac cacctataaa
    15721 gcgttagtcg aaatgcataa agcaggccag gtcagcttta agcacgttgt caccttcaac
    15781 atggacgaat atgtcggtct gccgaaagag catccggaaa gctactacag ctttatgcac
    15841 cgtaatttct tcgatcacgt tgatattcca gcagaaaaca tcaaccttct caacggcaat
    15901 gccccggata tcgacgccga gtgccgccag tatgaagaaa aaatccgttc ttacggaaaa
    15961 attcatctgt ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg
    16021 tcttctctgg cttctcgtac tcgtatcaaa accctgactc atgacactcg cgtcgcgaac
    16081 tctcgtttct ttgataacga tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt
    16141 ggtacattgc tggatgccga agaagtgatg attctggtgc tgggtagcca gaaagcactg
    16201 gcactgcagg ctgcagttga aggttgcgtg aaccacatgt ggaccatcag ctgtctgcaa
    16261 ctgcatccaa aagcgatcat ggtgtgcgat gaaccttcca ccatggagct gaaagttaag
    16321 actttaagat atttcaatga attagaagca gaaaatatca aaggtctgta attgttatcc
    16381 ctgccctggc tccttgctca gggcaatatt ttttaaaatc gggggtcaga atgtatgcat
    16441 taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg
    16501 ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac
    16561 gttcactgaa cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg
    16621 gcggcgtaca gtttaacgac accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc
    16681 agaaagccaa tgagaaatca ggctgtacta actatctgcc gacgcttatc accaccagcg
    16741 atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaaa catccgaatc
    16801 aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata
    16861 atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg
    16921 acgtcattac caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac
    16981 tggcaaatgc cgggattgtg gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa
    17041 aagccggttt ccgcgcgggg attacctttg ccacccatct gtacaacgcg atgccgtata
    17101 ttaccggtcg tgaaccgggc ctggcgggcg cgatcctcga cgaagctgac atttattgcg
    17161 gtattattgc tgatggcctg catgttgatt acgccaacat tcgtaacgct aaacgtctga
    17221 aaggcgacaa actgtgtctg gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac
    17281 agttcatttt tgcgggtaaa acaatatact accgtaacgg actttgtgtg gatgagaacg
    17341 gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt
    17401 gcggtatcgc actggatgaa gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg
    17461 gcgttgagaa acgtctcggc acactcgccg caggtaaagt agccaacctg accgcattca
    17521 cacctgattt taaaatcacc aagaccatcg ttaacggtaa cgaggtcgta actcaataag
    17581 agaaagtatg acaccaggcg gacaagctca gataggtaat gttgatctcg taaaacagct
    17641 taacagcgcg gcggtttatc gcctgattga ccagtacggg ccaatctcgc ggattcagat
    17701 tgccgagcaa agccagcttg cccccgccag cgtaaccaaa attacgcgtc agcttatcga
    17761 acgcgggctg atcaaagaag ttgatcagca ggcctccacc gggggccgcc gcgctatctc
    17821 catcgtcacc gaaacccgca atttccacgc aatcggcgta cggcttggtc gtcacgacgc
    17881 caccatcacc ctgtttgatc tcagcagcaa agtgctggca gaagaacatt acccgctgcc
    17941 ggaacgtacc cagcaaacgc tggaacatgc cctgttgaat gccattgctc agtttattga
    18001 tagctaccag cgcaaactgc gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt
    18061 tgacccggac agcggcaaaa ttcattacat gccgcatatt caggtagaaa actgggggct
    18121 ggtagaagca ctggaagaac gttttaaagt gacctgtttc gttggtcacg atatccgtag
    18181 tctggcgctg gcggagcact acttcggtgc aagtcaggat tgcgaagact ccattctggt
    18241 gcgtgtccat cgcggaaccg gggccgggat tatctctaac gggcgcattt ttattggccg
    18301 caacggcaac gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca
    18361 ctgcggcaac tttggctgcc tggaaactat cgctgccaac gccgctattg aacaacgggt
    18421 gttgaatctg ttaaagcagg gctaccagag ccgcgtgccg ctggacgact gcaccatcaa
    18481 aactatctgc aaagccgcga acaaaggcga tagcctggcc tcggaagtga ttgagtatgt
    18541 cggtcgtcat ctgggcaaaa ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat
    18601 tgttattgcc ggtgaaatca ccgaagccga taaagtgctg ctccctgcta ttgaaagctg
    18661 cattaatacc caggcgctga aggcgtttcg cactaatctg ccggtggtac gttctgagct
    18721 ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat
    18781 tttgctccag catttgctgg aaaattaatg tgcttttata gtggcgctta ttgttgtcaa
    18841 tattctgggt agtccatgac cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg
    18901 cacgataacg tcgccgtacc gggtgcagcg gaatttttgc acgggattat ggataaaggc
    18961 ctgccgctgg tgttgctgac caactatcct tcgcagaccg ggcaagatct ggcgaaccgc
    19021 tttgccactg ccggtgtcga tgtgccggat agcgtgtttt atacctctgc gatggcgact
    19081 gccgatttcc tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg
    19141 attcatgaac tgtacaaagc cggtttcact attaccgatg tgaaccctga ttttgtgatt
    19201 gttggcgaaa cgcgttccta caactgggac atgatgcata aagcggccta tttcgtcgct
    19261 aacggtgcac gctttatcgc caccaacccg gacacccacg ggcgcggttt ttatcccgct
    19321 tgtggcgcgt tgtgtgcagg gattgagaaa atctccgggc gcaaaccgtt ctatgttggt
    19381 aagcccagcc cgtggatcat ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa
    19441 acggtgattg tcggcgataa cttgcgcacc gacattctgg cgggcttcca ggcgggtctg
    19501 gagacgattc tggtgctttc tggtgtttca tcgctcgacg atatcgacag tatgccgttc
    19561 cgcccaagct ggatttaccc gtcggtcgct gaaatcgacg ttatctgaat agcaaccacg
    19621 tctcagggcg tggtttctta tttttaccca tcattaaaat catcccattc aataaaactc
    19681 gccaaaaatt atcgatccat caataaatac atcaccacac cctccccttt ttcacaaatc
    19741 agcaatcacc attacgtttt ttatttttgc actgccaaat cgcttgcgca cggtgctgct
    19801 ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa
    19861 ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa
    19921 gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta
    19981 tgccagcgat aacgccattc tcgcccacga acgtctgtca attgttgacg ttaacgcggg
    20041 ggcgcaacct ctctacaacc aacaaaaaac tcatgtgctg gcggtaaacg gtgaaatcta
    20101 caaccaccag gcactgcgcg ccgaatatgg cgatcgttat cagttccaga ccggatctga
    20161 ctgcgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca
    20221 gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcatatctga ttggtcgcga
    20281 ccatctgggg atcatcccac tgtatatggg ctatgacgag cacggtcagc tgtatgtggc
    20341 ctcagaaatg aaagccctgg tgccagtttg ccgcacgatt aaagagttcc cggcggggag
    20401 ctatttgtgg agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgactt
    20461 tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggcac tggaaaattc
    20521 cgttaaaagc catctgatgt ctgacgttcc ttacggtgtg ctgctctcgg gtggtctgga
    20581 ttcctcaatt atttccgcga tcaccaagaa atacgccgcc cgtcgcgtgg aagatcagga
    20641 acgctctgaa gcctggtggc cgcaattaca ctcctttgct gtaggtctgc cgggttcacc
    20701 ggatctgaaa gcagcccagg aagtggcgaa ccatctgggc acggtgcatc acgaaattca
    20761 cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca tcgaaaccta
    20821 tgatgtgaca actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaggc
    20881 aatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct
    20941 ttacttccac aaagcaccga atgccaaaga actgcatgaa gagacggtgc gtaaactgct
    21001 ggccctgcat atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct ggggcgtgga
    21061 agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca
    21121 ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc
    21181 atacctgccc gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta
    21241 cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctgatc agcaactgga
    21301 aactgcccgc ttccgcttcc cgtacaacac gccaacctca aaagaagcgt atctgtaccg
    21361 ggagatcttt gaagaactgt tcccgcttcc gagcgccgct gagtgcgtgc ctggcggtcc
    21421 ttccgtcgcg tgttcttccg ctaaagcgat tgagtgggat gaggcgttca agaaaatgga
    21481 cgatccgtct ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag aaatgcgaaa
    21541 cagccccggt gaaatccggg gctgtttcag ttattgaggt gcttgttaat tctgatatgc
    21601 agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag
    21661 tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc ctgtacgaca
    21721 aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac gaatatagcg
    21781 ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg
    21841 ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa
    21901 aaaaaagatg gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac
    21961 aggttcgaat cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc
    22021 accagattta ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat
    22081 tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata
    22141 ccggcattcc ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt
    22201 agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg
    22261 tagccaccaa attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg
    22321 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    22381 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcatt
    22441 caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg
    22501 tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca accctaacgc
    22561 atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta acccgagatt
    22621 acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca gatccatacc
    22681 actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata
    22741 actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca gggcatcgac
    22801 atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat gcgcggcatc
    22861 gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa
    22921 cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct tcgcgatttc
    22981 cgcctggagt tgcgccatat tcatattctg caactggcga atacgtaccg gagagtcata
    23041 ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt
    23101 aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa tcaacataca
    23161 cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca cctgcgaatt
    23221 tgcgccgtca gcaccaatca ccagcttcgc gcgaatcact tcaccgccct tcagttccag
    23281 ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac gtaacgttac
    23341 tttcggatgc gcttccagcg cctgccacaa tgcctgttgc aggacggtgt tttccaccat
    23401 atagccaagc agcggcagct taagttcagc ggcgtcaaac atcacatgcg ccgtttccca
    23461 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    23521 ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc gcacgtccgg
    23581 ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc
    23641 gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga caatggcaat
    23701 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    23761 gatttttacc cccaagggct gataacgctc atactggtca caacggcagc aaagcattac
    23821 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    23881 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    23941 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    24001 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg
    24061 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc
    24121 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    24181 gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc gcagcccggt
    24241 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga
    24301 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    24361 ggtgccttac acccgtggtg aagaggtaag ccgtccgtcc gacgatattc tgtttgagat
    24421 cgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc
    24481 ctggcgtggt gagaactacg acggcaccac cggatcgttt gccgatctgt tgcgtctggt
    24541 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    24601 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcatctgcc
    24661 ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtactcata cggcactgga
    24721 gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga tcagttctga
    24781 cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat
    24841 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc cgggtacacc
    24901 agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc tgtatattct
    24961 gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg gcaccaccca
    25021 gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga
    25081 aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat tcgtcgatgt
    25141 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga
    25201 aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc gcaaagaaaa
    25261 cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc ggtgggcctt
    25321 gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc ctgtggcgtg
    25381 aataatttcc ttataggccg gtgaatgttc agcttccggc gacatacaga taagaggaac
    25441 ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc gcgtctgttg
    25501 agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag
    25561 atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    25621 gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca gattcaggat
    25681 atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga gcaaagtgcg
    25741 gagagcgtgc cggtgtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    25801 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    25861 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    25921 ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga agccggtgag
    25981 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccgctg
    26041 tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat tgagcgcaac
    26101 gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc
    26161 attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct gacccgtatc
    26221 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    26281 actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga gatcagcttt
    26341 aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat cgttaacgcc
    26401 tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc agaacgcaag
    26461 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    26521 ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc
    26581 agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa agccacagtc
    26641 tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    26701 tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt caggtggttg
    26761 agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    26821 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    26881 aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    26941 aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    27001 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    27061 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    27121 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    27181 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    27241 cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    27301 ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga
    27361 agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag
    27421 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    27481 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    27541 tattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat
    27601 tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc gtcagctgag
    27661 tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg aagcgttcgg
    27721 cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg
    27781 gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca aagtggcgat
    27841 ggccgatagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    27901 aaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    27961 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    28021 cttctctcct tacgatgtct ggcctgcggc gattgtttcg ctgatggggc ttcaggcgct
    28081 gacctttaac cgccgcccgc tccagtctgc cgctattggc ttctgctggg gatttggcct
    28141 ctttggcacc ggtattaact gggtctatgt cagcatcgcg acctttggcg ggatgcctgg
    28201 cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt ataccggact
    28261 gtttgctggc gtgctgtcgc gcctgtggcc gaaaaccacc tggctgcgcg tggcgattgc
    28321 cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc
    28381 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccaataat
    28441 gggcgtggaa gccattaact tcctgctgat gatggttagc ggcctgctgg cactggcgtt
    28501 agtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc ttcccttccc
    28561 gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg tttcgatggt
    28621 tcagggcgat attccgcaat cactgaaatg ggacggagac cagcttctta atacgctgaa
    28681 gatttactac aacgcaacgg caccgctgct gggcaaatca tcgttgatta tctggccgga
    28741 gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga
    28801 gttgcgtgat aaaggtagct cgctggtaac cgggattgtc gacgcacgtc tcaataagca
    28861 gaaccgctac gatacctaca acaccatcat cacgctgggt aaaggtgcac cgtacagcta
    28921 cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct
    28981 ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg
    29041 tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg cggccatttg
    29101 ctacgagatc attcttggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    29161 gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc agcacttcca
    29221 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    29281 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    29341 cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg cacgtaccgg
    29401 caactggccg ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgc tgatgagtct
    29461 gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg tactttctca
    29521 ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga tacaagccac
    29581 attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg tcctgtccat
    29641 ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg aaaaacgaag
    29701 gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa tattgagcag
    29761 gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt ccgggctaat
    29821 accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg cttgttgatc
    29881 ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc cgttttcagg
    29941 tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt cttctttctg
    30001 atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa ccagtaatgt
    30061 tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg tccccgtccc
    30121 gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat caatcgctga
    30181 cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg agaggataaa
    30241 actgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg ccacgttaat
    30301 gaccgataac gcattcgctt caaatgacgt cgcataaatc aacttttcat ccgggcttaa
    30361 tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg acttttcttt
    30421 cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga aaacaacaat
    30481 gctgttggtg ttttcaattg cggcatacaa cgtattacca tcccgactga tcgccatatc
    30541 atggatatcg gcaatctcag cgagtaaatc accactggcc tgttcacgac ttaccggagc
    30601 ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgttgttttt tttgtcgtcg
    30661 ctcttgttca atctcctgcg gataaaacgc ttcacgcagg ctttgcctga cctgtaaaac
    30721 tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa gactgcccgc
    30781 aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg cgctcagcca
    30841 acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta ttcctgtttg
    30901 ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata ctcgccgctg
    30961 gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg caccagctat
    31021 tcccagaatc gcctcggatg ccccaacgct gatgactatc tgctcacttt cgcgtaacgc
    31081 ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa tcaggagtcg
    31141 ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa acatattcat
    31201 cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc agggttcacc
    31261 cgacaaagta agaggcgcaa cgtttgcacc gagatggata agtgcggatt gatcgacctc
    31321 ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg caaaaaggat
    31381 aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag acataaggtg
    31441 tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat catcccggca
    31501 aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc ctaaaattac
    31561 aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta tttactgtgt
    31621 aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag cggtagcacc
    31681 aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg caaaaacaaa
    31741 caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac aggcacaaca
    31801 ctgcacaata aagttgaaga cgataacaac acaaacactc acaacgggta tccatgcgtt
    31861 cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca cagcaatcct
    31921 cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg tagcgggcag
    31981 tacgctggac aaaatcgcca aaaacggcgt gattgtcgtc ggtcaccgtg aatcttcagt
    32041 acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg attactccaa
    32101 cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg taaaactgat
    32161 tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg attttgaatg
    32221 tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg atactatttt
    32281 cgtggtcggt acgcgcctgc tgaccaaaaa gagcggcgat atcaaagatt ttgccgacct
    32341 gaaaggcaaa gccgtagtcg tcacttccgg cactacttct gaagttttgc tcaacaaact
    32401 gaatgaagag caaaaaatga atatgcgcat cattagcgcc aaagatcacg gtgactcttt
    32461 ccgtaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc tgctggccgg
    32521 tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc cgcagtctca
    32581 ggaagcctac ggttgcatgt tgcgtaaaga tgatccgcag ttcaaaaagc tgatggatga
    32641 cactatcgct caggtgcaga cttccggtga agcggaaaaa tggtttgata aatggttcaa
    32701 aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc
    32761 actgttcaaa gaaccgaatg acaaagcact gaactaatta caagaaccag gggcggaaat
    32821 tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg tcgttcccca
    32881 tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag ttaacgctgc
    32941 taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt atttttttac
    33001 aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt tttcaggtga
    33061 cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca tttttcggca
    33121 ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat gtcgaactgt
    33181 tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc ccggagttgc
    33241 tgccagagaa aatcggtatg tggtttaagg ccgagctgga tcccaatatt cagtttttcc
    33301 tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg
    33361 ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg ggcctgacgc
    33421 taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc gtcccgccga
    33481 tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact attggtctgg
    33541 tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc tgggagtcgt
    33601 ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg gtaatgacgc
    33661 tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt acgagtttga
    33721 ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga tcacgctgaa
    33781 aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg cggtgatgcg
    33841 cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg tattccgctc
    33901 gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt ttctgcaaaa
    33961 cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc
    34021 aatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa gtatttctcg
    34081 tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga tgaaactgat
    34141 tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg gcatcgtact
    34201 gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc
    34261 aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctatttgccg gatttgttta
    34321 tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata
    34381 atgattactc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc
    34441 tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa
    34501 tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga aatcaccgtc
    34561 gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc ccgcgtcggg
    34621 atggtgttcc agcattttga gttgttcccc catttgtcga ttattgaaaa cctgactctg
    34681 gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc attgaaattg
    34741 ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct ttccggcggt
    34801 cagcagcagc gtgtggcaat cgctcgcgcg ttgtgtatgg atcctattgc gatgctgttc
    34861 gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga cgtgatggtg
    34921 gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc
    34981 aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga ctcgccgaaa
    35041 gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc gaaaatcctg
    35101 cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta tcgtttcgcc
    35161 cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca
    35221 acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc tatcgcaata
    35281 gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac
    35341 cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc
    35401 gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac
    35461 aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac gttcgcaccg
    35521 caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc ggaacctgtc
    35581 acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag ccatccggaa
    35641 ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct tggtaactgg
    35701 acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat tgtcttccag
    35761 tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca aatccacgtt
    35821 gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt tgccgaactg
    35881 ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg tgcaccgctg
    35941 catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac tgttgagcgc
    36001 tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt tgattattat
    36061 tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg tcagggcttt
    36121 gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt gattaagatc
    36181 accacgcgtt acctgggtgg tcttaatctg tttatctctt tttctttcaa gatataaatg
    36241 caatggataa tgcagaactc gccattggta tcgatctcgg cactaccaat agtttaattg
    36301 ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac
    36361 catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac
    36421 ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgtgcaatg ggcagtaata
    36481 ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac
    36541 gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct
    36601 ccgttccggc ttatttcagc gatgaacaac gcaagcatac ccgtttagca gcggagttag
    36661 ccgggttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcgtatggcc
    36721 tgcataccca acaaaatacc cgttcgctgg tgtttgatct cggtggcggc acgtttgacg
    36781 ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgacaact
    36841 ttcttggtgg cgaagatttt acccatatgc tggtcgatga ggttttaaaa cgcgcggatg
    36901 tcgccaggac cacgcttaac gagagtgaac tgggagcctt gtacgcctgt gtggaagcgg
    36961 caaaatgtag caatcaatcg ccattgcaca ttcgctggca gtaccaggat gaaacgcggg
    37021 agtgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc
    37081 gagtgccgat tgaacaggcg ttgcgcgatg cacgtctgaa gccgagtcaa atcgacagtc
    37141 tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt
    37201 ttggcaaatt accgtatcaa agttacgacc cgagcaccat tgtcgcgctg ggcgcagcaa
    37261 tccaggccgc ctgccgctta cgcagtgaag atattgaaga ggtgatcctc actgatattt
    37321 gcccttactc gttgggcgtt gaagttaacc gccagggcgt ttccggcatt ttctcgccaa
    37381 ttattgaacg aaacaccaca gtacccgtgt cgcgtgtaga aacttactcg accatgcacc
    37441 cggaacagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaata
    37501 acattctggt tgaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg
    37561 atattcgctt tagttatgat atcaacggct tgcttgaagt tgacgtgctt ctggaagatg
    37621 gcagcgttaa gtccagagtc attaatcata gcccggtaac attgagcgcg cagcagattg
    37681 aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaacc
    37741 gcacctttaa ggccaaactg gaagagttgt gggcgcgggc gctgggtgac gagcgagaag
    37801 agattggccg ggtgatcacc gattttgatg cggcgttgca gtcaaacgat atggcccgcg
    37861 tcgatgaggt caggcggcgg gcgagcgttt atttagccat tgagacctca taatcacggc
    37921 tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa
    37981 acaacatcgc gattttgccg agagcataac ctataaccag caccgttaaa ctgattttga
    38041 taatgctggt aatgctctgt ggtttttcac cctcatcatt tttttcttca tggttttcag
    38101 gtgttggctc atcgacatct tcatcctccg gctcgagttt tactttcgtc aacagcatcc
    38161 gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg
    38221 ccagcatctc ttgtttcgga aacgcttcaa gtactacctg ttccgctacc cgatacaaat
    38281 ccagtaacgg tgaatcttct tctgtctcgc taccgactct cgatttccac cacgcccatg
    38341 ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc
    38401 tatgtaaaat ttccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca
    38461 gcggttgacc ttgcgcatct accatgctcc gcgcttcgat aaccatcact aataacgcga
    38521 aataatccgg gcaatgacgc ttgctccact gcaacaacaa attatcagct tgtgtagatg
    38581 ggtaactttc ccggtacgcg cagagatcgg caagcaggct ttccccctcg ccgcagtaca
    38641 acctttgagc acacaagtaa tatcgggcat cttcgctttc tggttcttcc atttgccact
    38701 gtttggctac aggaatgagt tccgccatac ctgtttgcac cgagctgtac cagcgcaaca
    38761 gtttgcgatg taactttgca tcatcaggaa tgaaccacgg accttccatc gccaaaaacg
    38821 cagtgacgta gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tactcaacag
    38881 tctggttctg caacgcaacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag
    38941 cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa
    39001 acgaatgcca gttcaaacgc tctgccagca aagaagcaca atttaatgaa agatatcgcg
    39061 cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg
    39121 tatttaattg ctgaataaat ttctgccagg cggagggctg aaaacgatca ctttctgaat
    39181 ccagtaatgt cctgaatgca cgcagtattt catgttcagc ggcggcatct ttttcttcat
    39241 catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta
    39301 atgctttaaa cccctgcgga tcggtttcgg gatgacataa cggtaagcga gcaagataag
    39361 cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt
    39421 tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggctg
    39481 ccagggaagc gtggcataga aacgatcgct tcccgctgcc agctcttcga acatccgact
    39541 cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagtgt tgtcttcgca
    39601 ctgagaagat tgccagtaga gacgtcctaa cgaataggcg gaaaaatagt gcatccagct
    39661 gtgatagtaa tggtgagctc gtacatagac gcacgattgc agccataaac tctcttcttc
    39721 cgtcagccag ttgttgagca caccgacccg tgacagaaac cccattcgaa cataatccca
    39781 ggctttgatt cccccttctc cacagcacat agcggtttgg gcaacgaatt gggtataaat
    39841 caaccctctc tccgagccac cagcaattaa tgctttccac tcttcagggg aagagcgatg
    39901 ccatgtcaga tataagccag ctaaatcatt ggcatgacca tcatcagtca tctgataaat
    39961 agtctgaatt aaatcatcac gattatttat tccccaggat gatgagagat cgaattcttt
    40021 tatcccggta tacatttttg gcgagtcatg cgcgggtttg caaccctgat taagtatttc
    40081 catgggcgca gaaagcgcaa aaagaagata ttgttcttcc ttattcattt gttaataatc
    40141 ccttaagtgc attaactgac tactttttat tattgatgag tataaacttg acgctttcaa
    40201 aataaaaaga aaatcgaagc attcacatat gaataaaaaa ttaatgtata tattcgcaat
    40261 atttatagtt gcagcaatta cctgtattag ccaacccaag aaaacaacgt tgcgtgataa
    40321 agccatggtg aattatgcct ttgattattt aagctcaccg ggcagtctac cgtttaccac
    40381 ggcggccacg gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga
    40441 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
    40501 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
    40561 caaaggtctg ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc
    40621 tgaacaaggg tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg
    40681 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
    40741 caaaaatgcg gaaagattac ttgccgggct tcccgcacat taacgtcgac tgtcaaccga
    40801 acagatggag tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa
    40861 tgatgcccag acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg
    40921 gttcaacagt ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta
    40981 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
    41041 cttcggttgg ttggttcgcc tgcactaatt gtttggcaac taaatataat ggccataagg
    41101 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
    41161 cgaccagtga acgccccaga gcagagagtt tttcatcgct gagtatttcg tataaatgaa
    41221 aacgcgtacg ggcgctgtcg ccggggatat aaagtagcgg ttttccctga tcgttaacca
    41281 gttcacgagc ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc
    41341 accattgcaa tattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa
    41401 gttctgccag caagctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
    41461 gcgcatcttc attatcagat tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
    41521 gaattcccag gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
    41581 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
    41641 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
    41701 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
    41761 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
    41821 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
    41881 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
    41941 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcacga aaggcgagca
    42001 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
    42061 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
    42121 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
    42181 tcgtttcctc tatttcgaga atcttccagc aatttttcat aggtcgctca tatccttaag
    42241 cgtttccggg catgcagaat agtaagccaa tggttgccag gacaaggtag ggtaatacgc
    42301 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
    42361 cgctaagaat tcgcgtaata actgcagatg atcatcttcg ggagactgcc aatattgccg
    42421 accaaaggta taaccggcaa agtattgccg ccagttacga taataacgta gagcccgtag
    42481 atgaatgcgc gactggatcc atagactttc ttcttcgctc aaccagttat taagtacgcc
    42541 cattcggctt aaaaaaccca tccggacata atcccaggct ttaattcctc cctcaccaca
    42601 gcattcggcg gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc
    42661 aagatagtca cgccattcac atggcgaata acgaaaccag cggtgataaa accctgccaa
    42721 ccgagcggca tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacgggc
    42781 atcaattccc caggaggaag aaaggtcaaa aaagcatgta cctaaataca ttttgggtga
    42841 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
    42901 tattttttgc gactccatgt ccatataatt tatccttaaa tattcattaa atgaatatta
    42961 gccgcattgc gaggctcgtt tatttatact tttctgacgc gacataatac gcataaaata
    43021 aggcttacgc aaaaatggaa cacataaaaa tataatatgt tttatctgca accataagga
    43081 aatgataatg attttcacgt caagttgctg cgataattta tcaatagatg agattatcga
    43141 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
    43201 tagcgcaatt gattctccgg acgacgaaaa agccttttac tggctgaagc tggccgctga
    43261 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
    43321 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
    43381 tttcgcttcc aacgcccttg gctggacact ggatcgtgga gaagccccca attataaaga
    43441 agcggttgtc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
    43501 tgggtggatg tacagaaatg gcaacggagt cgcaaaagac tatgcgctgg catttttctg
    43561 gtacaaacaa gctgcattac aaggccatag tgacgcgcaa aacaatctgg ccgatcttta
    43621 tgaagacgga aaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
    43681 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
    43741 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
    43801 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gtgttgagaa
    43861 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
    43921 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
    43981 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
    44041 tcgtgagact gaagatttac tgtagagata aaaacaaggg ccggggaatt tcattcggcc
    44101 catattctct ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga
    44161 actggtcatg accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
    44221 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
    44281 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
    44341 tatctgccag ctcctgccat aagctttctt taatcacccg cataaagacg ctgtcagatt
    44401 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt cttacagctc
    44461 gcgtcagctc atcgacctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
    44521 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ctcagtgacg
    44581 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
    44641 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
    44701 aaattaatgt atgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
    44761 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
    44821 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
    44881 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
    44941 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
    45001 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg
    45061 caaaaacgtc ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
    45121 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
    45181 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
    45241 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
    45301 taagaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
    45361 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
    45421 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
    45481 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
    45541 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
    45601 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
    45661 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
    45721 tgccgaagct gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt
    45781 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
    45841 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
    45901 taaatacggc ctgaacatca aaccagttat cctggctgct gacggctctg agccagatct
    45961 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgaat tcaacggtct
    46021 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
    46081 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
    46141 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct
    46201 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
    46261 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
    46321 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
    46381 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
    46441 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
    46501 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
    46561 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
    46621 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
    46681 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
    46741 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
    46801 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
    46861 gaaacgtgtc tggaaactgg tttacgagca cacagcaaaa ggtgatgttg cggcactgaa
    46921 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc
    46981 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
    47041 ggagctgatg aacaaactgg cgaaagcacc aaccgatggc gagcaggatc gcgctctgat
    47101 gcaggaagcg ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt
    47161 cacgctgtgg caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
    47221 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
    47281 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
    47341 ccaggaacat ctggtagcaa aatatcttga tggcgttacc gtgcgtaaag tgatttacgt
    47401 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
    47461 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
    47521 gataccacgc aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac
    47581 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
    47641 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
    47701 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
    47761 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagtgccaa agtcttccgt
    47821 tcgttctttg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
    47881 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
    47941 gcggatattc gttccgacaa agaacagacg tcgaccacaa cggatactcc ggcaacgcct
    48001 gcacgcgtct ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg
    48061 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
    48121 tgcaggaaag ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc
    48181 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
    48241 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
    48301 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
    48361 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
    48421 cgaatcgcag cgtgcaggtg atctgtcaga caccggagca ggctcagctt ccccgctggg
    48481 ttgctgcgcg cgcaaaacag ctcaacttag aactggatga cgctgcaaat caggtgctct
    48541 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc tctggagcgt ttatcgctgc
    48601 tctggccaga cggcaaattg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
    48661 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
    48721 atattcttca gcaactgcgt ctggaaggca gcgaaccggt tattttgttg cgcacattac
    48781 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg
    48841 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
    48901 atcgcttaag tcagacgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
    48961 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
    49021 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
    49081 ctgtttggcg gcacctttga tccggtgcac tatggtcatc ttaaacccgt ggaaacgctg
    49141 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
    49201 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
    49261 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg
    49321 caaacactga aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt
    49381 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
    49441 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
    49501 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
    49561 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
    49621 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtact gacttacatt
    49681 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
    49741 taattcgtca tggtgaaacg caagcgaata tcgatggtct ttacagcggt catgcgccca
    49801 tccccctgac cgcgcgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
    49861 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgacggg
    49921 ttctcagtga ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg
    49981 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
    50041 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
    50101 cgcaacgtgt ggaaagattt atcgcaaggc ttagtgaata tcagcactat cagaatattt
    50161 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtctaatt ggcatgcctg
    50221 ccgaatctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    50281 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    50341 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    50401 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    50461 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    50521 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    50581 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    50641 gatgacctca aaggtcagga catcatcgcc ttagacgttc agggcaaatc cagcatcacc
    50701 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    50761 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    50821 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    50881 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    50941 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    51001 ccgaaagata tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg
    51061 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    51121 cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    51181 ctggaacgct ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg
    51241 ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc
    51301 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    51361 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    51421 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    51481 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    51541 ctgcaaattg ttcgctttac cgattaccag acccgctcta atgaaaaccg cattaagctg
    51601 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggcactc
    51661 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    51721 ctggacgctt tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa
    51781 gagcgcgcac gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta
    51841 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    51901 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa
    51961 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    52021 acgcatgata tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    52081 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    52141 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    52201 attgaaacgc tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt
    52261 ggggtgctgg cactggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    52321 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    52381 acacaggggg tttatcctcc tgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    52441 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    52501 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    52561 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    52621 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    52681 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    52741 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    52801 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    52861 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    52921 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    52981 gttgctaacc gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    53041 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    53101 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    53161 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    53221 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaatac cgatctgcct
    53281 gcggaaaatc ctgcggttgc cgcagcggag gaccattaat catgacggat aatccgaata
    53341 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat
    53401 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    53461 agcgtaaaat aggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    53521 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    53581 tggcggtaga tgctttcggt gccatctcta aaggcgctca acgctggctg gacctcggta
    53641 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    53701 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    53761 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    53821 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    53881 cagtagtgct ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc
    53941 agcgccagcg cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc
    54001 acattattca gtctaaaatt gctattggct ccggtggatt acgcggcaaa ggctggctgc
    54061 atggcactca gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg
    54121 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    54181 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    54241 ctggcggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    54301 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcagcgctaa
    54361 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    54421 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    54481 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    54541 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    54601 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    54661 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    54721 aacctgacgg cctctggcga agctttcgat ccgacacagt tgacggcggc ccatccaacg
    54781 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    54841 cgcattaatg atcgcggccc ctacggcaac gaccgcgtaa tttcgctttc tcgcgcagca
    54901 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    54961 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac
    55021 gccctgcctg cacctcccga tttaagcggt ggcgcggggg caagttcagt gtctggcccg
    55081 cagggtgaca ttcttccggt cagtaactcg acgctaaaaa gcgaagatcc gaccggcgcg
    55141 ccggtaacca gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa
    55201 agcagcgaac cgacacctgc accacagccc gttgttacag ctccgtcgac aacgcctgca
    55261 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    55321 gtgcaagtcg gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    55381 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg tatccagctt
    55441 ggcccattcg ccagtaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    55501 caattacagt catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    55561 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    55621 gctatagtag ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac
    55681 cattttttcc gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt
    55741 tatctctgct gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca
    55801 gatcgatgcg gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca
    55861 gaacgcagat gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat
    55921 cggccaggca atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga
    55981 cgcatgggcc accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg
    56041 catgcaggtt ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc
    56101 ttgcgtcgcc atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa
    56161 cagctacgtt aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga
    56221 tgctgatggt cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg
    56281 tgacgtaccg aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg
    56341 ccagctgaac cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac
    56401 cggacacact gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg
    56461 cttgatttct gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa
    56521 actgctaacc tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga
    56581 gttcgcctct gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa
    56641 agacgtgtac ctgaccattc cacgtggtcg catgaaagat ctgaaagcca gctatgtgct
    56701 gaacagcagt gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt
    56761 ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga
    56821 aggtaacttc ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg
    56881 ttaaaaatta aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa
    56941 aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    57001 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    57061 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    57121 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    57181 aacgccactc atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt
    57241 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct
    57301 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    57361 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca
    57421 ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt
    57481 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    57541 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    57601 acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    57661 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    57721 tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt
    57781 acgaattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    57841 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    57901 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    57961 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    58021 atttacactg ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt
    58081 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgcaa ctcaccccat
    58141 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    58201 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    58261 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    58321 aatatttaca accttacgaa atattgatct taaccttctg actatttttg aagctgtata
    58381 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag
    58441 tcagtctatt cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca
    58501 gggtgtcact cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga
    58561 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    58621 tgcgaccact ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac
    58681 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    58741 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    58801 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc
    58861 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaaga
    58921 gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa
    58981 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    59041 gattattccc agccgttttt ataacctgtt tagccgctgc tggccgctgg aaaaactgcc
    59101 ttttccgtcc ttaaatgagg agcaaataga tttctctatc cactacaaca aattcagcct
    59161 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    59221 tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca
    59281 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    59341 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    59401 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc
    59461 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc
    59521 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    59581 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    59641 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    59701 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    59761 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    59821 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta
    59881 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgtg
    59941 gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata
    60001 acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat tacaactggt
    60061 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    60121 tgatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    60181 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    60241 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    60301 tgggctttac ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc
    60361 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    60421 aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag cgtcctgctc
    60481 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    60541 cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    60601 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    60661 tagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    60721 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgtgcac acaatagcaa
    60781 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    60841 aagcgacggt tgtttaagac gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    60901 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    60961 tctataatcc ggctttgacc gatatttttg ttcccgcact cccccgccgc caccatataa
    61021 caggtggtat ccagcgcacg agcggcaagc aacgttgacc aatgatgctc tttgagcggc
    61081 ccgcgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc ctgtgccagc
    61141 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccctccacc
    61201 tcgagtaacg gagcgatttc attaccagca tcaacacggc gtgattcctg aatggcaaat
    61261 gcatcataga gatgcaattt ggcataacgg gcgacgatgt ttcctgcctg tagcgccacc
    61321 agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc
    61381 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    61441 gatttaaccg atagatctgc atcatgatca tcgcgcgcca gcaatgcttc cggcaggacg
    61501 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    61561 ttttcccaca cagatgtaac agcaaactgt cctgcggcaa ccagcattgc cacctcctgt
    61621 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    61681 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    61741 gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccgctgcatc aggcgattcc
    61801 gttggggacg ctggcagcaa acctgattgg ggcattcatc ataggaatgg gattcgcctg
    61861 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    61921 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    61981 ccgctttggc tgggcgttac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    62041 agcactggct ttctggctgt tttcggcctc aaccgcacac taaagaccaa aaaaaacccg
    62101 ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc
    62161 agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt
    62221 tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc
    62281 cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt
    62341 agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg ttaccagtac
    62401 accaattgtg gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta
    62461 tttttgatgt tacttatttt agctattgat tttaaagaag ttactaaaac ttcatttgtc
    62521 tcaaaactga aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat
    62581 gtaggtcatc tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg
    62641 gagagggatg taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa
    62701 atgaaaattt tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg
    62761 ttcccatttg ttccacgagt ccctaaatgc catggcatac aggccatgcc agtttccttt
    62821 ttcatcccaa cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt
    62881 tttttctttg tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata
    62941 atgttcaggc tgttgccagg tttgtgcaat attttctcta aacgttgtca tccactcatc
    63001 tgcgttagca aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc
    63061 gacatattta ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc
    63121 atagcaaagc tacaatta
//
LOCUS       DAZUFB010000016        58584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZUFB010000016 DAZUFB010000000
VERSION     DAZUFB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.016"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..447
                     /locus_tag="ACHY3M_001183"
     CDS             <1..447
                     /locus_tag="ACHY3M_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFS4316135.1"
                     /translation="HEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLILQSIKTKL
                     LSSRLNKFNDFEDSFFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLD
                     VTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            444..935
                     /locus_tag="ACHY3M_001184"
     CDS             444..935
                     /locus_tag="ACHY3M_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFS4316136.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLIV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(977..1927)
                     /gene="corA"
                     /locus_tag="ACHY3M_001185"
     CDS             complement(977..1927)
                     /gene="corA"
                     /locus_tag="ACHY3M_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFS4316137.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2079..2135)
                     /gene="ysgD"
                     /locus_tag="ACHY3M_001186"
     CDS             complement(2079..2135)
                     /gene="ysgD"
                     /locus_tag="ACHY3M_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFS4316138.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2297..3061
                     /gene="yigE"
                     /locus_tag="ACHY3M_001187"
     CDS             2297..3061
                     /gene="yigE"
                     /locus_tag="ACHY3M_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFS4316139.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3208..5370)
                     /gene="uvrD"
                     /locus_tag="ACHY3M_001188"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3208..5370)
                     /gene="uvrD"
                     /locus_tag="ACHY3M_001188"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFS4316140.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5454..6170)
                     /gene="yigB"
                     /locus_tag="ACHY3M_001189"
     CDS             complement(5454..6170)
                     /gene="yigB"
                     /locus_tag="ACHY3M_001189"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFS4316141.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6170..7066)
                     /gene="xerC"
                     /locus_tag="ACHY3M_001190"
     CDS             complement(6170..7066)
                     /gene="xerC"
                     /locus_tag="ACHY3M_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4316142.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7063..7770)
                     /gene="yigA"
                     /locus_tag="ACHY3M_001191"
     CDS             complement(7063..7770)
                     /gene="yigA"
                     /locus_tag="ACHY3M_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFS4316143.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7767..8591)
                     /gene="dapF"
                     /locus_tag="ACHY3M_001192"
     CDS             complement(7767..8591)
                     /gene="dapF"
                     /locus_tag="ACHY3M_001192"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4316144.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8628..8831)
                     /gene="yifL"
                     /locus_tag="ACHY3M_001193"
     CDS             complement(8628..8831)
                     /gene="yifL"
                     /locus_tag="ACHY3M_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4316145.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9181..9735
                     /locus_tag="ACHY3M_001194"
     CDS             9181..9735
                     /locus_tag="ACHY3M_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFS4316146.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10073..11581
                     /locus_tag="ACHY3M_001195"
     CDS             10073..11581
                     /locus_tag="ACHY3M_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS4316147.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWDDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11578..12903
                     /locus_tag="ACHY3M_001196"
     CDS             11578..12903
                     /locus_tag="ACHY3M_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS4316148.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAVTDGQTLKP"
     gene            12951..13271
                     /gene="cyaY"
                     /locus_tag="ACHY3M_001197"
     CDS             12951..13271
                     /gene="cyaY"
                     /locus_tag="ACHY3M_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFS4316149.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13311..15857)
                     /gene="cyaA"
                     /locus_tag="ACHY3M_001198"
     CDS             complement(13311..15857)
                     /gene="cyaA"
                     /locus_tag="ACHY3M_001198"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFS4316150.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            16244..17185
                     /gene="hemC"
                     /locus_tag="ACHY3M_001199"
     CDS             16244..17185
                     /gene="hemC"
                     /locus_tag="ACHY3M_001199"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFS4316151.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            17182..17922
                     /gene="hemD"
                     /locus_tag="ACHY3M_001200"
     CDS             17182..17922
                     /gene="hemD"
                     /locus_tag="ACHY3M_001200"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFS4316152.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17944..19179
                     /gene="hemX"
                     /locus_tag="ACHY3M_001201"
     CDS             17944..19179
                     /gene="hemX"
                     /locus_tag="ACHY3M_001201"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFS4316153.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            19182..20378
                     /gene="hemY"
                     /locus_tag="ACHY3M_001202"
     CDS             19182..20378
                     /gene="hemY"
                     /locus_tag="ACHY3M_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFS4316154.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            21057..21194
                     /locus_tag="ACHY3M_001203"
                     /pseudo
     CDS             21057..21194
                     /locus_tag="ACHY3M_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21302..22537)
                     /gene="aslB"
                     /locus_tag="ACHY3M_001204"
     CDS             complement(21302..22537)
                     /gene="aslB"
                     /locus_tag="ACHY3M_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS4316155.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(22684..22760)
                     /locus_tag="ACHY3M_001205"
     tRNA            complement(22684..22760)
                     /locus_tag="ACHY3M_001205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22724..22726),aa:Pro,seq:tgg)
     gene            complement(22803..22889)
                     /locus_tag="ACHY3M_001206"
     tRNA            complement(22803..22889)
                     /locus_tag="ACHY3M_001206"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22853..22855),aa:Leu,seq:cag)
     gene            complement(22910..22985)
                     /locus_tag="ACHY3M_001207"
     tRNA            complement(22910..22985)
                     /locus_tag="ACHY3M_001207"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22950..22952),aa:His,seq:gtg)
     gene            complement(23044..23120)
                     /locus_tag="ACHY3M_001208"
     tRNA            complement(23044..23120)
                     /locus_tag="ACHY3M_001208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23084..23086),aa:Arg,seq:ccg)
     gene            complement(23223..24608)
                     /gene="yifK"
                     /locus_tag="ACHY3M_001209"
     CDS             complement(23223..24608)
                     /gene="yifK"
                     /locus_tag="ACHY3M_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4316156.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24799..25539)
                     /gene="wecG"
                     /locus_tag="ACHY3M_001210"
     CDS             complement(24799..25539)
                     /gene="wecG"
                     /locus_tag="ACHY3M_001210"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFS4316157.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQFKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25542..26894)
                     /gene="wzyE"
                     /locus_tag="ACHY3M_001211"
     CDS             complement(25542..26894)
                     /gene="wzyE"
                     /locus_tag="ACHY3M_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFS4316158.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26891..27970)
                     /gene="rffT"
                     /locus_tag="ACHY3M_001212"
                     /gene_synonym="wecF"
     CDS             complement(26891..27970)
                     /gene="rffT"
                     /locus_tag="ACHY3M_001212"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFS4316159.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(27967..29217)
                     /gene="wzxE"
                     /locus_tag="ACHY3M_001213"
     CDS             complement(27967..29217)
                     /gene="wzxE"
                     /locus_tag="ACHY3M_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFS4316160.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29219..30349)
                     /gene="rffA"
                     /locus_tag="ACHY3M_001214"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29219..30349)
                     /gene="rffA"
                     /locus_tag="ACHY3M_001214"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFS4316161.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30354..31028)
                     /gene="rffC"
                     /locus_tag="ACHY3M_001215"
                     /gene_synonym="wecD"
     CDS             complement(30354..31028)
                     /gene="rffC"
                     /locus_tag="ACHY3M_001215"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFS4316162.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(31006..31887)
                     /gene="rfbA"
                     /locus_tag="ACHY3M_001216"
     CDS             complement(31006..31887)
                     /gene="rfbA"
                     /locus_tag="ACHY3M_001216"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4316163.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31906..32973)
                     /gene="rffG"
                     /locus_tag="ACHY3M_001217"
     CDS             complement(31906..32973)
                     /gene="rffG"
                     /locus_tag="ACHY3M_001217"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4316164.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32973..34235)
                     /gene="wecC"
                     /locus_tag="ACHY3M_001218"
     CDS             complement(32973..34235)
                     /gene="wecC"
                     /locus_tag="ACHY3M_001218"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFS4316165.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34232..35362)
                     /gene="wecB"
                     /locus_tag="ACHY3M_001219"
     CDS             complement(34232..35362)
                     /gene="wecB"
                     /locus_tag="ACHY3M_001219"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFS4316166.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35418..36464)
                     /gene="wzzE"
                     /locus_tag="ACHY3M_001220"
     CDS             complement(35418..36464)
                     /gene="wzzE"
                     /locus_tag="ACHY3M_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFS4316167.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGGWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36476..37579)
                     /gene="wecA"
                     /locus_tag="ACHY3M_001221"
     CDS             complement(36476..37579)
                     /gene="wecA"
                     /locus_tag="ACHY3M_001221"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFS4316168.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37819..39078)
                     /gene="rho"
                     /locus_tag="ACHY3M_001222"
     CDS             complement(37819..39078)
                     /gene="rho"
                     /locus_tag="ACHY3M_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4316169.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39163..39264)
                     /gene="rhoL"
                     /locus_tag="ACHY3M_001223"
     CDS             complement(39163..39264)
                     /gene="rhoL"
                     /locus_tag="ACHY3M_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFS4316170.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39405..39734)
                     /gene="trxA"
                     /locus_tag="ACHY3M_001224"
     CDS             complement(39405..39734)
                     /gene="trxA"
                     /locus_tag="ACHY3M_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFS4316171.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39865..41130
                     /gene="rhlB"
                     /locus_tag="ACHY3M_001225"
     CDS             39865..41130
                     /gene="rhlB"
                     /locus_tag="ACHY3M_001225"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFS4316172.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41264..42748
                     /gene="gppA"
                     /locus_tag="ACHY3M_001226"
     CDS             41264..42748
                     /gene="gppA"
                     /locus_tag="ACHY3M_001226"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFS4316173.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42795..44816)
                     /gene="rep"
                     /locus_tag="ACHY3M_001227"
     CDS             complement(42795..44816)
                     /gene="rep"
                     /locus_tag="ACHY3M_001227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFS4316174.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            45033..45482
                     /locus_tag="ACHY3M_001228"
     CDS             45033..45482
                     /locus_tag="ACHY3M_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4316175.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45681..45962
                     /gene="ppiC"
                     /locus_tag="ACHY3M_001229"
     CDS             45681..45962
                     /gene="ppiC"
                     /locus_tag="ACHY3M_001229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFS4316176.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(46049..47524)
                     /gene="ilvC"
                     /locus_tag="ACHY3M_001230"
     CDS             complement(46049..47524)
                     /gene="ilvC"
                     /locus_tag="ACHY3M_001230"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFS4316177.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47674..48567
                     /gene="ilvY"
                     /locus_tag="ACHY3M_001231"
     CDS             47674..48567
                     /gene="ilvY"
                     /locus_tag="ACHY3M_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFS4316178.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSIVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48619..50163)
                     /gene="ilvA"
                     /locus_tag="ACHY3M_001232"
     CDS             complement(48619..50163)
                     /gene="ilvA"
                     /locus_tag="ACHY3M_001232"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFS4316179.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50166..52016)
                     /gene="ilvD"
                     /locus_tag="ACHY3M_001233"
     CDS             complement(50166..52016)
                     /gene="ilvD"
                     /locus_tag="ACHY3M_001233"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFS4316180.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52081..53010)
                     /gene="ilvE"
                     /locus_tag="ACHY3M_001234"
     CDS             complement(52081..53010)
                     /gene="ilvE"
                     /locus_tag="ACHY3M_001234"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFS4316181.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(53030..53293)
                     /gene="ilvM"
                     /locus_tag="ACHY3M_001235"
     CDS             complement(53030..53293)
                     /gene="ilvM"
                     /locus_tag="ACHY3M_001235"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFS4316182.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53290..54936)
                     /gene="ilvG"
                     /locus_tag="ACHY3M_001236"
     CDS             complement(53290..54936)
                     /gene="ilvG"
                     /locus_tag="ACHY3M_001236"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFS4316183.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54939..54989)
                     /gene="ilvX"
                     /locus_tag="ACHY3M_001237"
     CDS             complement(54939..54989)
                     /gene="ilvX"
                     /locus_tag="ACHY3M_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFS4316184.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(55076..55174)
                     /gene="ilvL"
                     /locus_tag="ACHY3M_001238"
     CDS             complement(55076..55174)
                     /gene="ilvL"
                     /locus_tag="ACHY3M_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFS4316185.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            55527..57047
                     /gene="yifB"
                     /locus_tag="ACHY3M_001239"
     CDS             55527..57047
                     /gene="yifB"
                     /locus_tag="ACHY3M_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFS4316186.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(57072..57410)
                     /gene="maoP"
                     /locus_tag="ACHY3M_001240"
     CDS             complement(57072..57410)
                     /gene="maoP"
                     /locus_tag="ACHY3M_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFS4316187.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            57529..58368
                     /gene="hdfR"
                     /locus_tag="ACHY3M_001241"
     CDS             57529..58368
                     /gene="hdfR"
                     /locus_tag="ACHY3M_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFS4316188.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(58464..58539)
                     /locus_tag="ACHY3M_001242"
     tRNA            complement(58464..58539)
                     /locus_tag="ACHY3M_001242"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58504..58506),aa:Trp,seq:cca)
BASE COUNT        14505 a        15643 c        14778 g        13658 t
ORIGIN      
        1 catgaaaaaa taaataaata tggttataat agtccaatac gaataatcaa agaatgtatc
       61 gaaaatggca ttaattcaac tccctcgaca ggataccaac ctcttatcct gcaaagcatt
      121 aaaaccaaat tactttcatc aagactaaat aaatttaatg acttcgagga cagttttttt
      181 aatggcttag gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt
      241 caaaaatacg ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt
      301 cttgatgtaa ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg
      361 ttctttttac aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact
      421 atcgaaagaa tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat
      481 ttcttctatg ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat
      541 actctgatgc tggcaattca gtctttctga tcgttgatca tctgccatgg actgacagcg
      601 ataaaataaa ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag
      661 aaggtagttg gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa
      721 aatatatcga agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata
      781 attgcatcgt gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata
      841 ttggtattaa cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt
      901 tcaatccgca tgactttaaa aaagacaatt tttagaaata agccggataa aaaaatagct
      961 ttatccggca ctcatattac aaccagttct tccgcttaaa gtacagatac ggtgccaggc
     1021 ccgcgaggat cataaagata atcgcgcctg ggtagccgaa actccacttc aactctggca
     1081 taaattcaaa gttcatcccg tagctggaag ccaccagcgt cggtggcagg aacaccacgg
     1141 ataccaccga gaagattttg atgatgcggt tctgctcgat gttgataaag cccatcgccg
     1201 cctgcatcag gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga
     1261 tatctcgcag gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gccttgcgta
     1321 ccaggaagtt gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat
     1381 cttccagttc cgccagcgtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca
     1441 taatcacccg gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact
     1501 gttcgatttt ggtttcgaac agatccagca gcaactcgta ggcgttaccg tctaccatcg
     1561 actggctacg ggcacgcata cgatacagac gaaaagcagg cagttcacgc tcacgcagag
     1621 taaacagacg accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg
     1681 catcttcaaa gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg
     1741 ccgatgcttc gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt
     1801 gtacgcgcag tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa
     1861 ggggttgtga ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc
     1921 tcagcatgac cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa
     1981 caaattgggt ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg
     2041 ctgacaaccg ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac
     2101 aggatagtga gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg
     2161 tgcgcgcatg ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca
     2221 atagttgaac ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca
     2281 acatccagga cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa
     2341 tctcaaacgg atttttctca ccctcacctt acttcccttg tttgctgttg ccgctgatga
     2401 ttgcgcactc tcagatccga cgctgaccgt acaggcgtat accgttaatc ctcaaacaga
     2461 gcgggtgaaa atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct
     2521 gctggcggat atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga
     2581 tgaaagctat gcgccgctcg gtttgtacat cgaaaacggt cagcagaagg tggcgttaaa
     2641 tctcgcttca ggtgaaggga atttctttat ccgtcctggc ggcgtgtttt atgtcgcggg
     2701 agataaagtc ggcatcgttc gtctggatgc cttcaaaacc agtaaagaga ttcagtttgc
     2761 ggtgcagtca gggccaatgt tgatggaaaa cagtgtaatt aatccgcgta ttcatcccaa
     2821 cgtcgcctca cgtaaaattc gtaacggtgt tgggattaat aaacatggga atgccgtgtt
     2881 tttgttgagc cagcaggcga caaattttta tgattttgcc tgttatgcca aagcgaaact
     2941 gaacgttgag cagctgcttt atcttgatgg cacgatttca catatgtata tgaaaggcgg
     3001 ggcaatcccg tggcaacgct atccttttgt caccatgatt tccgtagagc ggaaaggtta
     3061 aaacgatagt tttgtgtcag atactgaaga tggcgcagat gccggatgcg gcgtaaacgc
     3121 cctatccggc ctacatgacg ttgcaattta ttgaatctga atgatttttt aggccaaata
     3181 aggtgcgcag caccgcatcc ggcaacgtta caccgactcc agccgggcat aagccgccac
     3241 cagccattta ataccctggc cctgaaatgc cacctgcaaa cggctatgct caccgctgcc
     3301 ttccatattg acaatggtgc cttcaccaaa cttagcgtgg cgtacgcgct ggccgagctt
     3361 gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctga ccgggcggct
     3421 taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc caataaagcg
     3481 cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt aggtcagcgt
     3541 cagtttctgc atcgcgcggg ttacgccaac gtaggccaga cggcgttctt cttccagacg
     3601 cccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac cgacgataaa
     3661 cacctgcggg aactccagac ctttcgccga gtgcatggtc atcagttgta ccgcatcttg
     3721 ccaggtatcc gcctgccctt cgcctgcttc cagcgccgca tgggatagga acgcctgcag
     3781 cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg tcaccagttc
     3841 ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt acatggtacg
     3901 caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat cggcagtttc
     3961 ctgcgctaag gcgtcgatca gttccataaa ccgctgtaag gcgctggcag cacgtccggc
     4021 gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact ggcgatcgcg
     4081 cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg gcgtattcac
     4141 cacacgctca aaggccgcgt cgtcgttacg gttggcaatc aggcgcagat acgagagcgc
     4201 atctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt acggcatact
     4261 cgcctgcaac agtgcttctt ccagcacacg cgactgggcg ttactgcggt agagaatggc
     4321 gcactcggca agcgccccgc cgttgtcctg ccaggttttg atgcggttaa ccacaaaacg
     4381 cgcttcatcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt ccgcgccatc
     4441 ggtccacagt tttttaccca aacgcccgtt gttgttttca atcagggcgt tcgcggcgct
     4501 cagaatattg ctggtagagc ggtagttctg ctccagacgg atagtttcag caccggggaa
     4561 atcattgagg aaacgctgaa tattctccac ctgcgctcca cgccagccgt agattgactg
     4621 atcgtcatcg ccaacgatca tcactttgcc ggtatcgccc gccagcaggc ggatccacgc
     4681 atactgaatg ttgttggtat cctggaattc gtccaccagg atattggtaa accgttcgcg
     4741 atagtgctga aggatatgcg gcttgttaag ccacaactcg tgagcacgta gcagcagctc
     4801 ggcgaagtcc accaggcccg cgcggtcaca cgcttcctga tacgcctgat acaccttctg
     4861 ccaggtctgc tccaccggat taccgtagct ttgaatatga tgtggacgca ggccttcatc
     4921 tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct cgtcgaggtt
     4981 catcgccttg attaggcgtt tgagcaggcg tagctggtcc tcgctgtcga ggatctgaaa
     5041 atcctgcggc agattggcgt ccatatggtg cgcacgcagc agacggtgcg ccagcccgtg
     5101 gaaggtgccg acccacatgc cgccctggct ggtgcccatc agctgcccga tacgatgacg
     5161 catctccgcc gccgctttgt tggtaaacgt caccgccata atcgagtatg gcgagcagtt
     5221 ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac cactgcccgc
     5281 gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct gtttgtcatt
     5341 aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat aggttgggcg
     5401 gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttgc tgattatatc
     5461 agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctgtcccag
     5521 gtttgcatca gatcgccatt ttccggtctg atccaacagg cctgcattcc gctgcgaatt
     5581 gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg
     5641 ttgagttttt ccgcagccag aaagtacata tcgctgaacg gttttgagcg cccgtgcggg
     5701 ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgggcgtta
     5761 ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt gtcgtgagtt
     5821 tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt tgatcattgc tgcgtgtgcg
     5881 cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga
     5941 aaacgccagc gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc
     6001 aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag
     6061 gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc
     6121 aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat tatttccccc
     6181 gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa tcaagatgag
     6241 tatagatttg cgtggtggag aggttggcat gacccagtag ctcctgcaca ccacgaagat
     6301 cgccgctcga ctccagcata tgcgtagcga acgagtgacg taatttatgt gggtgaacgt
     6361 gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca ttacgcgcgg
     6421 agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca aacaggtcgc
     6481 gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc aggcggcgct
     6541 ctttgctgcc tttccccata acccacactt caccagactc caggtcgagg tgtttaatat
     6601 ccagccccac cagctcagaa agacgcagac ccgcgccgta catcacttcc agcattgcac
     6661 ggtcgcgtac agcgagggga tcgttgatat caatatccag cagccgattc atatcgtcga
     6721 cgtcgatgtt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct ttcgccgggt
     6781 tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc gcagaaagcc
     6841 gtaacgccag acttgctgct cccagccctt tacggcgact gcgtacagca aaattgcgca
     6901 ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca aaattgatga
     6961 tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg cgctccacgc
     7021 tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc gttcaatcca
     7081 acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg ttccttgccc
     7141 ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat cagcatcgct
     7201 tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca ccaccagcag
     7261 ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct gaatacgcag
     7321 cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg cacctaagcg
     7381 ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc gcgcccagcg
     7441 gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc gctgcaggta
     7501 gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca gcgccatgtt
     7561 ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga ccaacgaaac
     7621 ggtgccgcgt accggatgcg gcacacgtat cgcttctact gcgcgcgcat tacggataaa
     7681 aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct ccgtgagtgt
     7741 ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccgtcgta gacatgtacc
     7801 gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc aagacgaccg
     7861 ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc tactgcaacc
     7921 gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc ataaacgcgt
     7981 aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg ctccggaaaa
     8041 cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg
     8101 acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg
     8161 gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac ggcggaaggt
     8221 tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc
     8281 atccgcccgt tggcggtgct gacgcggata tcacgcttat tggtcagtcc tttcagacgc
     8341 acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact gccatcagca
     8401 ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac caccagcagt
     8461 tggtcaaacc ctacccccag gtgccgatca gccaggcgac gaatcagctc cggtgaaaaa
     8521 aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc
     8581 gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc
     8641 tgggatggac catcgccagt ggcgcgatcg tttttatccg gcaccgtgga ttgcgtttgc
     8701 gtctctaccg gtttggtcgg cggcggtgcg tttttatctg caggcgggaa atagagcgga
     8761 cctttcagac cgcagcccgt caggctgaag agagtaatta atacagtgag tgccttaaac
     8821 acgtttttca ttatggattg cctgtaaagt tcatcgcttt ctgctttcta tcatcgcagg
     8881 agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt gaggaaatgc
     8941 tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg tgatcggcac
     9001 ctcgcgccag gatggctcta tgcactatcg caatgtagtt tgtttgttgg cttgttcgtt
     9061 gttcttatcg tcggcatgga gcttttaaaa gagtggcgca ccatgcaatt ttgttgtctg
     9121 accttagtcg ccagtgacat acaactttgc caacctggtg ataagaggga ataaaatctg
     9181 atggggctat ttacccaact ggaaaaattt gaccaaaagc ttacgcgcgg ttatgcccgg
     9241 tgggggcgct gggtatggcg gacgttaatt gtcgttccgg tactggtcgt gttgtggaat
     9301 ataggacagg cagtatgggg aggaccaaga ggtggagtaa ttctggaaat ccacagtgaa
     9361 attgaccgcc cgatactggg attcagtgtt aacggcgtgg cgggcgcgaa tgcttttgca
     9421 aatggtggcg gttcaacaac ctgttgtggt gatgttagtg gggatacggc agaggttatc
     9481 tggacattaa gtactactcg tacacagtat aatgctggaa tgcgtctgga gaaacggaat
     9541 atgacgttac ctctccccaa aagagaatgg ggagaagatt ttctgcatgt gcattttatg
     9601 cctggcgata aagtgctgct ggggtggagt aaagattctt tttctccgta tgaagatctt
     9661 cacaacgggg ggtacaagac ccgcgttcgg caggatgtga aagataaact ttatggtaca
     9721 gggaaaatga attgatagat ttctataaaa atcacgttca gcagaaagcg taataatgtg
     9781 gggaatgtta gataccggtg gtgggaccta agtacaacac gcgaaagaca ataaagtagg
     9841 taacggtggg cagagtcgca tgttgacacc cttcccggcg tcaatccggc acattttaac
     9901 gtgatcagtc acaccatggc gcagtagctc gtatttatat cgtgaatgaa aacctcaagt
     9961 ttaccccagc aactatcaga aaatagcgat ggcctttata gattctgaaa agtgacgatt
    10021 tgccgcaaaa gttacgctta cgtgcgagga attatgttct gctggtagtt tcgtgatcgg
    10081 cacctcgcgc caggatggct ttatgcacta tcgcaatgtg gtttgtttgt tgtcttgttc
    10141 attgttttta tcgtcggcat ggggatgtcg gcttgatccg cctgaacata atatttacca
    10201 gaaacaggga aaaggcgtgg tatacctgcg cccttataaa gaaacaaatc tttctcttcc
    10261 tgacgtgaat tacaaaagtt tgcgccgttt acccaattta ttaatagacc cgacaacact
    10321 agacaaatgg gataaagtac ctcctctgac tgatctgaca accgactacc tctatgaagg
    10381 cgcacaggcc tggtatcctc attactcctg gcatagcgat ggtcgctata tcctctacgc
    10441 gggtgaagtt gtgcaaaacc cgccagacaa acctcccgtc gatgtcgcct cgttcaaggc
    10501 gtgggatgat tttgccgcag ataaacacag tctctatttc gaaggcaagc gcaccgataa
    10561 caatggcggg ggaaatcgct tagatatcaa aacactgcat caggtggaat tccggcctcc
    10621 ctgggatcct gacttactgg ggctgatatt acgtgacgct aatttcttgt atatcaatgg
    10681 gcatcgcctc gcagatcccg atagtttccg tgtgctggca caaaagtcat gggatcagcg
    10741 aggaaagttt tctaccgcgt ttaatccctg tgtggctatg ccatttggcc cctgggatac
    10801 cctggctcgt acacggacca aaatcctgat caacggcgaa cagcttgatg ccgacccgga
    10861 caccttttcc gtcgtgcgct ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg
    10921 gctgcagcgt aaagtcctcg acaaggaaaa tctggagtgg gatgaagatt taacaaagca
    10981 ctgtctggat ttttctctgc tggaaaagaa agtgttctgg cgtaaaggac ctgcttgtaa
    11041 acaggaagaa ttacccggac tcgatccgga gcagtttcaa cccatcagtg atgctgtcgc
    11101 ccagtatcag gactcgcttt ataccatcat cgaaacagag tctggtgacc gcaagctgga
    11161 aatcgtgaaa cttgatgatc ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg
    11221 ccacggctat ttacttacgc gtgccgaagg gtggccatac cattccggtt tacatgtgtt
    11281 tgaatctgac ggaccgctga tcttactgga taaccgttct ccggatgaac gcgaagccca
    11341 tcttaatgac catccctttt tgcgcagatg gtatgcccgc gataaccgct acatttacag
    11401 ctttgatggc gcgcagctct ggcgataccg caccgctgat ccgaaacaag ttcgcttaat
    11461 ctggaaggaa caacattcgg gatatggcta tggcgtaaat tacaaaacgg gatatctgga
    11521 cggaaaaatt actgatgacg gcgaatttat tcctgccccg cgcaatgagg cgacaaaatg
    11581 aagggtcaaa taatcacagt tgcactcatc ctgttgggac acacatttct gacgcctgct
    11641 gtacaggcca tagggttcga ctattacaat gatcacggtg ttatgtctta cggtaaaggt
    11701 tattgggggg ataagaaaat cattgcccag tttccggaga tgagcagggc cgatttgcgc
    11761 cttgtgacaa atatatccgg ggagcgggag ttggtggaag gctatatacc caccgatgaa
    11821 ataagcataa aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
    11881 ggcaaaatcg ttagcaatcc accgggaaca cccactgtcg atattgccag ctttcaggct
    11941 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
    12001 aatagcggcg cgtcacgtgt tgatctggcg acactaaaag ctatcgaagg taactctacc
    12061 acgctggtgg atagtaaaaa tctctatttg tccggtcgac gtcaggggag cagcagtaat
    12121 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
    12181 aatttgtatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
    12241 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
    12301 gtttttcgcg acaataaggg tatgcatcgt tatccctttg ggcaattgtc tggcaaagcg
    12361 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
    12421 cagctcacgt ccgaccgtca gtggagcaag tggatagacc taccagatat tgaacctgaa
    12481 caattccatc tgattactgg caatattgcg caatataaag atcggctgta tgtaacaaaa
    12541 ttatcgacat ttggtgaaga ccagcttgag ataatcccgc tggatacgcc agacctggtc
    12601 attgatcgct catttaatag cggcaaacag catgcttatt ttatccgcca attacggtca
    12661 aagagcgtgc aaattattcc agttaacggt ccgctaacta aaaacgatcg cttcgcttat
    12721 gacgatcgca atgtttatac atggaccgat acagaggtaa ggattacgcc ctccccctgc
    12781 ccggcgaaaa cacacgtcag agaagagaat gtgcgtgaac ttcacaacag agacattatt
    12841 attccgttga cggatgactc atgccggaac gcagtaacag acgggcaaac tttgaagccc
    12901 tgaccccata ccgctatact gccgccatca cttaaagaca ggatacaaca atgaacgaca
    12961 gtgaatttca tcgcctggct gatcaactct ggctgaccat tgaagaacgc ctggacgact
    13021 gggatggcga cagcgatatc gactgcgaaa tcaacggcgg cgtactgacc attacctttg
    13081 aaaacggcag caaaatcatt attaaccgcc aggagccgct gcaccaggtg tggctggcaa
    13141 ccaaacaggg cggctaccat tttgacctaa aaggcgatga atggatttgc gatcgcagcg
    13201 gcgaaacctt ctgggatttg ctggaacagg cggcgacaca gcaagcgggt gaaacagtca
    13261 gtttccgcta agattgcatg ccggataagc ctcgctttcc ggcacgctca tcacgaaaaa
    13321 tactgctgta atagcggcgt atcgttgtcc tgattggcgg gcggcatgtt accgatagat
    13381 tttgtgcgga acggaatcac ctgttcacga ccatcaacct tcacaatctg atagaactgc
    13441 ggcaggttga agttgataaa gcttgagccg taggtgaagc gatcgtgcga tgacgagtag
    13501 aagcgactga cgtcacgtac cagctcctct ttgctgcctt cgcagtggtg atacacctca
    13561 acccggttgc tttcgtcgag aatgtagata ttaaagccat tctcgtcttg cgtttcttcg
    13621 aagaagaact ggatgatccc ttcgctggca aagccgtcca ccaccgccgg taatttgacg
    13681 tgattggttt caacctgcac tgacaagccg tgcagtttgt tatgcgaaat agcgccataa
    13741 aactcgatgg cgttttccag tttttgtacc gatacattca ggcgttcgaa gaacaacccc
    13801 caggtttgac cagaaacgcg cagcgccttg aaacgcccgg tttcctggcg ggtgctggaa
    13861 aggcgcagtt caatacactc agaaaccagt tgctgcacgc gagtacggat taacccgcgc
    13921 agatgctggc tataacagaa gacttccacg ctatctggcg gtgcggcgtc ctgatgcatt
    13981 ttgccgagaa tagttttcag ggcttcgatc atcgattgct cgccgttgaa gtgcagcgta
    14041 cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc tacctaccag gcaattttga
    14101 ttctcgccaa agctgaagac atccagctta cggaaatcaa aatgcaccac ctgattgcgg
    14161 aacgccgctg tcgggtcata ttccaggtta acgataatcg ccagatggcg gatctcacac
    14221 gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg gtgcgacacg
    14281 tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt aatatacaaa
    14341 cgggtgcgcg aggtcagcag gccgttaaac catgcccacg ccaccagttt gttcaggtaa
    14401 cggttatatt ccagcggctg atggctgatg atcgactcaa tatttggcgc gcggttatac
    14461 agataccaac ctgaacggtt agcacggccc ggcggcacat aaataaaggt cagattcggt
    14521 tccgagagat cgggtgaaat ctgcgggttt accagcgtca ctttacctgg taatgcttca
    14581 aacgcggcat acagcttacg cgtcagcacg ccgatatcct gcggactggc ggagacgcta
    14641 aggttattgc gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat cgcgtcgagc
    14701 aactcgttgt gcgcctcacg cacctgatca atcttccagt tagcgcggtt atcgagcatt
    14761 gccagacgag cttcgtccca accccactcg ctcactaact ggctcaacac tgcgcgacgc
    14821 cagcctacgc aggcgcgttc acggctgagc ttttcgcaca cttttaaata gaagcagcga
    14881 cgtactaaat ccagacgggt aaaatcttca atcgccgtca ggtattcagt aacacgctcc
    14941 agcatcatgc agtatggatc gagaccaaac gatacaatct cgccgtcgtg caaacgctgt
    15001 ttgatatctt tcgccagcag acgtgggttc gggtattccc aggaataggc ttccagcagc
    15061 agtgttttca gtaccgcttt gtatggggaa tcgatactct tgtagagctg ccaaaggctg
    15121 gcaccaaagt actcttcagc agaaagcgag cttaagccac cgagatccag ccattcattt
    15181 ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt cgtcgtaatg ctcttcttcg
    15241 tcgcacggca ccatattcca cagaatacgc ttaccggcga gacgcacggc ggtacgataa
    15301 aattcgtcaa gcagcagtat atgctgggtg gagccacaat cttcgccccc caggctgccg
    15361 ctttcattat gacggaagcg gttttcatca atcaggaaga agctgacttc cacacccagc
    15421 gaggcggccc agctttccag cagactacat ttacgttgta gtaactggcg ctcttcgcta
    15481 tcgagccagg attgatgaca gacccagata tccaggtcag aggaacaact ttgccctacg
    15541 gacgaggtgc tgcccatggt gtatacacca gtaattggaa gctcaccttt cggtgggtcc
    15601 tgtactgaca ttccacgata cagttcaagc tcgttcaggt agtggcgttg agtttcatca
    15661 ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg cattagcgga
    15721 tggtgatagt gcaacaatgt cggcagtaga ctgtagacct gttggaatgc aggccccata
    15781 gcagcaagcg cgcgatccac acgcaattga tttatggcat ccagtctctg tttcagagtc
    15841 tcaatataga ggtacaagac gtatcgcctg atttgctacc cgtcatgact gtgattccgc
    15901 caacatcaac ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt aaccgttacc
    15961 acttttcgcg cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa cgtgatcaat
    16021 ttaacacctt gctgattgac cgtaaagaaa gatgcgctac atacaagtgt agcaccgttt
    16081 attctctgta aattccttat tacgacggcg tgaaacgcct gtcaggatcc actgccagac
    16141 ctcattttac ggtttgcgca ggcgtctacg tttcaccaca acactgacat cactctggca
    16201 aggatgttag gatggaccac ggatgataat gacggtaaca agcatgttag acaatgtttt
    16261 aagaattgcc acacgccaaa gcccacttgc actctggcag gcacactatg tcaaagacaa
    16321 gttgatggcg agccatccgg gcctggtggt tgaactggta ccgatggtga cgcgcggcga
    16381 tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa ggcttatttg taaaagagct
    16441 ggaagtcgcg ctcctggaga atcgcgccga tatcgccgta cattcaatga aagatgtgcc
    16501 ggttgaattc ccgcaaggtc tggggttggt cactatttgt gagcgtgaag atcctcgcga
    16561 tgcctttgtg tccaataatt atgacagtct ggatgcgtta ccggcaggca gtatcgtcgg
    16621 gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc tgattatccg
    16681 ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta gataacggcg aatacgatgc
    16741 catcattctt gccgtagccg gactaaaacg tttaggtctg gagtcccgca ttcgcgccgc
    16801 gttaccaccc gagatttctc ttccggcggt aggacaaggt gcagtcggta ttgaatgccg
    16861 ccttgatgat acacgcactc gcgagctgct tgccgcgctg aatcaccacg aaactgcact
    16921 gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa ggtggatgtc aggtgccaat
    16981 tggtagctac gccgagctta ttgatggtga aatctggctg cgtgcgctgg tcggcgcgcc
    17041 ggacggttcg cagattattc gcggtgaacg ccgcggtgcg ccgcaaaatg ccgaacaaat
    17101 ggggatttcg ctggcagaag agctactgaa taacggcgcg cgcgagatcc tcgctgaagt
    17161 ctataacgga gacgccccgg catgagtatc ttggtcaccc gcccgtctcc cgctggagaa
    17221 gagttagtga gccgtctgcg cacactgggg caggtggcct ggcattttcc gctgattgag
    17281 ttttctccgg gtcgacaatt accgcaactt gctgatcaac tggcggcgct gggggagagc
    17341 gatctgttgt ttgccctctc gcaacacgcg gttgcttttg cccaatcaca gctgcatcag
    17401 caagatcgta aatggccccg actacctgat tatttcgcca ttggacgcac caccgcactg
    17461 gcgctacata ccgtaagcgg acagaagatt ctctacccgc aggatcggga aatcagcgaa
    17521 gtcttgctac aattacctga attacaaaat attgcgggca aacgtgcgct gatattacgt
    17581 ggcaatggcg gtcgtgagct aattggggat accctgacag cgcgcggtgc tgaggtcact
    17641 ttttgtgaat gttatcaacg atgcgcaatc cattacgatg gtgcagaaga agcgatgcgc
    17701 tggcaatccc gcgaggtgac gatggtcgtt gttaccagcg gtgaaatgtt gcagcaactc
    17761 tggtcgctga tcccacaatg gtatcgtgag cactggttac tacactgtcg actattggtc
    17821 gtcagtgagc gtttggcgaa actcgcccgg gaactgggct ggcaagacat taaggtcgcc
    17881 gataacgctg acaacgatgc gcttttacgg gcattacaat aactctcata acaggaagcc
    17941 ataatgacgg aacaagaaaa aacctccgcc gtggttgaag agaccaggga ggccgtggac
    18001 accacgtcac aacctgtcgc aacagaaaaa aagagtaaga acaatactgc attgattctc
    18061 agcgcggtgg ctatcgctat tgctctggcg gcgggtgtcg gtttgtatgg ctggggtaaa
    18121 caacaggccg tcaatcagac cgccaccagc gatgccctgg ctaaccaact gacggcattg
    18181 caaaaagccc aggagagcca aaaagccgag ctggaaggca ttattaagca acaagctgca
    18241 cagcttaagc aggcgaatcg tcagcaagaa acgctggcaa aacagttgga tgaagtccaa
    18301 caaaaggtcg ccaccatttc cggcagcgat gctaaaacct ggctgctggc tcaggccgat
    18361 tttctggtga aactcgccgg acggaagctg tggagcgatc aggacgtcac gaccgctgca
    18421 gcgttgctga aaagtgcaga tgccagcctg gcggatatga atgacccgag tctgattacc
    18481 gttcgtcggg caattaccga tgatatcgcc agcctttctg cagtttcgca ggtggattat
    18541 gacggcatca tccttaagct taatcagctt tcaaatcagg tagataacct gcgtctggcc
    18601 gataatgaca gcgatggttc gccgatggat tccgacggtg aagagctttc cagttccatc
    18661 agcgaatggc gtatcaatct gcaaaaaagc tggcagaact ttatggacaa cttcattacg
    18721 attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc caaatcagga tatctatctg
    18781 cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg tcaccaggaa
    18841 gagacttatc gccaggcgct ggagaacgtc tccacctggg tacgagctta ctacgatact
    18901 gatgatgcca ccaccaaagc gttcctcgac gaggtggacc agttaagcca gcaaaatatc
    18961 tcgatggatc ttccggaaac cctgcaaagc caggcgatgc tggaaaaact gatgcagacc
    19021 cgcgtgcgta acctgctggc acaaccggca gcggggacaa cggaagctaa acctgcacct
    19081 gcacctgcac ctgcacctgc acctgcacct gcacctgcac ctgcacctgc acctgcaccg
    19141 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
    19201 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
    19261 acgtgctgat ccagactgac aactacaata tcgaaaccag cgtcacgggc ctggcgatta
    19321 ttttgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
    19381 gcacaggcgc acacacccgt gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc
    19441 agaccgaaca ggcgctgctg aaactggcgg aaggcgatta tcagcaagtt gaaaagctga
    19501 tggcgaaaaa tgccgatcac gcggaacaac cggtggtgaa ctatctactg gctgccgaag
    19561 ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc
    19621 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
    19681 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
    19741 cggaagtatt acgtctggcg gaacaggcgt atatccgcac aggtgcatgg agttcgctgc
    19801 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
    19861 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
    19921 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg
    19981 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
    20041 tcgatggcct gaaacgccag tacgacgatc gcctactgct gccgattcct cgactgaaaa
    20101 ccaacaatcc ggaacagctg gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc
    20161 gcccgctgtt gtggagcaca ctgggccagt cactgatgaa gcacggagaa tggcaggaag
    20221 catcgctcgc cttccgcgcg gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
    20281 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
    20341 gtttgatgtt aacgttgcag aacaacccac cacagtagtt ccttctcacc cggaggcaag
    20401 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt
    20461 aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg
    20521 atgaggcata agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt
    20581 ccggagttta tgaggcacta aggcgaacat aagagatgga atgagcatct acccgtttat
    20641 tatgccacag agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa
    20701 tcctctttgc ttcctgagta ataacttcct gagtgaatat ttaacctgag cttgatccta
    20761 cacatactta ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt
    20821 taaaactaaa aaaagcatca ataatcaacg cgatataata aacctgcctt acatatcaac
    20881 tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat
    20941 aaataattta tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca
    21001 attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg
    21061 aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg
    21121 ccagcgcagc agataccccg tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc
    21181 acccaaacca gtatccacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
    21241 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt
    21301 tttacttact caccaccagc aaatgcgcat gcataatgtc actggccggg cgaccgtgcg
    21361 ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc
    21421 cggcacacaa ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg
    21481 ggcagcctcc ccagcacgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct
    21541 taaatttatc ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt
    21601 gctgatgcat attccccagc cgatattgcg gataaacata gtgatcgcag gcgtaaacgt
    21661 cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac
    21721 ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga
    21781 cgtcgcgttt gatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc
    21841 gcaccgacca ttccgtcagt tccccctgaa tatcaccagg cgcatgtagt ttcagtccat
    21901 cgctggcagc tgtttcatca gccaggcgct cgaccaccgg aataaactgg atgaattcga
    21961 ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg gtgcggttga
    22021 cgcagaccag cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca
    22081 ccagcttatg cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg
    22141 gaccgtccag cgataaccca acaagaaaat gattttctgc cagaaatgca caccatttat
    22201 catcgagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccgtatt
    22261 tcgcctgtaa ctttacggca cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc
    22321 cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat
    22381 acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt
    22441 tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccactc ggtttcgcca
    22501 tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cactccttgc ctgaagtcat
    22561 ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat
    22621 tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa
    22681 aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta
    22741 ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat tctttaaagt
    22801 cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct
    22861 agcgcgtcta ccaattccgc caccttcgca ttgtcacaac ttctaataat ggggtggcta
    22921 atgggattcg aacccacgac aactggaatc acaatccagg gctctaccaa ctgagctata
    22981 gccaccacta caaatcttgt tacgcggtat tactaccacc gcagctcaag cgccgggact
    23041 aaatggcgcg cccgacagga ttcgaacctg agacctctgc ctccggaggg cagcgctcta
    23101 tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg acttcccacc
    23161 cctctgtcta gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct
    23221 gattattcct ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc
    23281 gccgtcaccg ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta
    23341 aagtacatgc cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg
    23401 aacaggattg agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc
    23461 agctggctta tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag
    23521 acgtagacaa acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc
    23581 agaatagcaa tagatactgc cacacccgca actggtacgc cgtgacggga aactttcgcc
    23641 atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac
    23701 atgccgctgt tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc
    23761 gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt
    23821 tcattccacg ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc
    23881 cgccacagca ctttgcctac ggcactgcgc agcgtcacct gcggattctt cgcttcaccg
    23941 gcagtaatgc caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc
    24001 aggaaccctt tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca
    24061 atcgactgcc cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc
    24121 acgataatcg tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc
    24181 accgccgcca gattcgccaa cgccaccagc gccactgcga tcaatgcggg tatccactgc
    24241 gccatctccg ggaaccagaa ctggacataa acgccaatgg cggtgatttc tgagatcccc
    24301 accgccatcc acataaacca gtaagaccag gcggtgagat aaccaaagaa cgggctcata
    24361 taacgatgcg cataaacggc gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc
    24421 attgaacgca tgatgaaaaa gacgaatagc ccggcgatga tataggccaa caatacggat
    24481 ggcccagccc atttcaaggt actggcggcc cccataaaca ggccgacgcc aatggtgccc
    24541 cccagggcga tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta
    24601 tctgccataa atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg
    24661 ggtacatcga gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa
    24721 aaatttgaat gctttgtgta ataaaaaagc agacaggcga cggagtgacc actccgtcgc
    24781 tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa
    24841 acgaagctga cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagtcc
    24901 cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc
    24961 gccaacgccc atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc
    25021 ctgctttggc gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc
    25081 aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac
    25141 attccactgg ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac
    25201 aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc agagatcagc
    25261 accggcaacg cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct
    25321 gatgccatcc gcatatttaa attcggcagc gttaattagc tccctgacct cagcattatc
    25381 ttcaatggtc agcatttttt cggcattaat ggcaaccagc gttccctgct taaactgccc
    25441 gtcagcaaac agataatcga gggcgtgctg catatcacgc caaccaatca actgtaagcc
    25501 acgcagcgta taggtcggtg ccatggtgtt gttattcatt gttatccttc aacctgcgtc
    25561 cggagcgatg attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacaac
    25621 agttttgcga tcatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca
    25681 aacgaatcca gcccttcacg tgccagcacg atcatattga agatcgcccc aaagcagaaa
    25741 ctgtgcaata tcgcagcctt atagcgatta ggctcgcggt tgcccagctc atacagccag
    25801 tcgaaccatt tgatgatcag accaaccacg atcgccccga gcgggatgaa caacgcgccg
    25861 cccatcacca ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc
    25921 agcacttccc aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac
    25981 agccaggaag ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg
    26041 atgttgtcgt agttctgcaa cagcaacgcc agattctccc acggcgagaa ggtgtcgcga
    26101 gtgagataga gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt
    26161 agcgccagcc agaacatgcc cacaatcccc agcacgcccg ccgccgccag catccacaac
    26221 gaaatccagc cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg
    26281 gcgcgagtgc cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg
    26341 aaaaacagcc acgctttgct atcctggcgc agaaagtaga ccaccagcat cgccgggatg
    26401 aaaaagtaaa agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg
    26461 taggagttaa gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc
    26521 agcgcgatgc ccatcaggat cacccacgta agattggtct ccacgcggtt catggtaaac
    26581 agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag
    26641 taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg
    26701 acaccaacat caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg
    26761 agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac
    26821 caggtcagcg tggcaataaa cagcgtgcag agcagccaga ccacaaacag gccactgaat
    26881 tgcagcagac tcatgcgccc tccccggcgg caatcgccaa cgcccgctgc cagccttgta
    26941 gatagttagg gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg
    27001 cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa cagcactggc aaatgctgtt
    27061 ccgtcatatc ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa
    27121 tcagtaagca cagcgtacca atgccctgct ggcgagcaaa aataaagtaa ccaagatcgc
    27181 actgacgaag tagcgccaga taggcgtcaa attccagttt ttcgctcaaa atttgcagat
    27241 tttcttcgct gaataactcc agccccgcct gacgaacttc ctcaatgtac gcttcgttat
    27301 taggcggata tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg
    27361 cacgtaaagc ggcgacatgc tcattgctga ggtcgccgga gttccccacc agaatggtca
    27421 tttttccttc acgttgccga tcgttcgcca tcgtattgag agaagggtcc atccgcgtcg
    27481 ggaagtacag cagttctccc cgcacctttg ggtgcgtttt ggcaaaaaag ctcaaatcgc
    27541 cgcgggtggc aaatacacag ccgactcgct tttgcgccag gcgacgtagt gggtaaaaaa
    27601 gcttatatct caagccactg gaaagctcat acaggtctgc cccccagata tgccagaaaa
    27661 actggctggg cttaatgcca ccactcagca gagccagcca cagtgtggga ttgaactgac
    27721 cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca
    27781 gcgatttttt cccagggaaa aattgcacag aaagcgccgg acagctatca cttaagccgt
    27841 cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct cgtcgcagcc agcgcgtcat
    27901 tgaaaaaccg caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca
    27961 gtacagtcat gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac
    28021 gatataagtt gccatatatg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca
    28081 gtgggcaaat accatcaata aagtgaactg gctgatttcc gccagaatat aaaaccgcaa
    28141 tgacgctttg gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc
    28201 cactaactgc caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcagcca
    28261 gatagcaaaa tcacgcagca gccagacggt aaaactcgcc gccgccactg ccggtaagac
    28321 gaatttcagc gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa
    28381 cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat
    28441 actgctcacc ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt
    28501 acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa
    28561 tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat
    28621 gacaccacgt ttgatcaaca tgatagtggc aggaattacc accagcgcgg gaatcagcgc
    28681 cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac
    28741 gccaatcaag ctgccgacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt
    28801 catcagcgcc agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg
    28861 caccagcccc tgatagtcgg tattaccaaa caatccctgg ctgattggcg cagctgccag
    28921 cacaaaaacc agcgccatca gcgtagagaa accaagtacc atcgctgatg aagtgccgac
    28981 cacgcggcgc agctgttgcg gattatcatg gtactgggca acgtatttgg ttacaccgtt
    29041 aaagatgcca gctccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc
    29101 cagcccaagc cccgccggac caaatgacac agccagcaac ttaccgacca gtaacccggc
    29161 accaatcttg accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc
    29221 aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt
    29281 agaacagcgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt
    29341 ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg
    29401 ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca
    29461 gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga
    29521 tacgcccggc tttcgccaga ggcgctaacg catcgtagta gttttgccac agcgccagac
    29581 gttgctggtt gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat
    29641 cggacatcaa atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga
    29701 agaactggct gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt
    29761 tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt atagtttttg gtttcatgga
    29821 agctaaagca gccaatatga ccaatggttc ccagtgcacg ccctttgtaa gtggacatca
    29881 cgccctgagc ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg
    29941 tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg
    30001 tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa
    30061 aaacgatttt tgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg
    30121 gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag
    30181 cggtgcagga cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct
    30241 ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca
    30301 tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg
    30361 tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg
    30421 gcggtgttgc ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag
    30481 tttagcgccg tttgcatcag ctcagcacct acaccgcgtc cagccagcag gccaattcgc
    30541 gcatctgtcg cattgagttc ccgtaaagag acataaccgc gaataccgcc ggacgccgca
    30601 cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca
    30661 taaaagcgtc tgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat
    30721 gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcacca
    30781 ctatcactga cattgttcac gggtagtgcc aaatcaactt caccttctac cagggagaat
    30841 cccagctgtt gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc
    30901 cgtgaccacg gcgctaacac gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg
    30961 atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg
    31021 acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc
    31081 agcacgcttc acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca
    31141 ggcaatctta aagccctggc gtttttctac tgtctgcaca aaggtactgg cttcaatcag
    31201 gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt caacggtcag
    31261 gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga
    31321 cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac
    31381 cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc
    31441 gtcaaactcc accacgccaa agcgttccgg gtccatcacc tgatagccaa ataccgtcgc
    31501 cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat
    31561 gttatcgccc agcaccagac aagaaggttc accgttgagg aaggtttcac cgatgataaa
    31621 ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat tccagctgga taccgaactc
    31681 actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag
    31741 aatttcgcgg ataccggcca acatcagcac cgacagcgga tagtaaatca ttggcttatc
    31801 gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga
    31861 accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc
    31921 taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccagcttt cattagccag
    31981 ataccactgc accgttttac gcattccact ttcaaaggtt tcctgtggca gccaaccaag
    32041 ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg gacggtcagc
    32101 gacaaaggtg atcaagtcac gatagtgcgc cacgccgtgc ggcttgttcg gagccagttc
    32161 ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc
    32221 aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg
    32281 atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcggttt
    32341 acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc
    32401 gtagttattc gagcagttgg tgatcagcgt cggcagaccg taggtccgca gccaggcgcg
    32461 caccagatgg tcgctactgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt
    32521 ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cggtggagat
    32581 atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt aagcccgcgc
    32641 cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc
    32701 aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc
    32761 agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagtg
    32821 ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac
    32881 cactaccacc gcatcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa
    32941 cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt ggcatcgacg
    33001 acatactgct gatggacatt gtcgccattg ataactttga actgactatg atcgaccagc
    33061 atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg
    33121 gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagcgtttc gccgctgtgc
    33181 cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc
    33241 ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagca
    33301 gcaaggcagt cggcaaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg
    33361 ttaacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac
    33421 cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg
    33481 cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa
    33541 atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc
    33601 ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg
    33661 tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt
    33721 ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg
    33781 acgtccgcct gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac
    33841 cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg
    33901 ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc
    33961 tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc
    34021 gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca
    34081 ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg gttgatatcg
    34141 acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa
    34201 cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg
    34261 cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag
    34321 tttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat
    34381 ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc
    34441 gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg
    34501 tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga
    34561 tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt
    34621 tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcgatg tctgccagcg
    34681 cgtggcagat ttcttcaaag ccacgaccga aactttcacg cctgtgaccg gtcaccagaa
    34741 tcatcttttt atcggggtcg ataaacgggt aatttgccgc cagttctgaa cgcagcgtgt
    34801 cgctgctcat cacctgatca cgcacccata acagtgcatc aatgactgta ttaccagtaa
    34861 tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg
    34921 gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg
    34981 gcgaatagag atcgcccgtg cgtaaaccag cctcaacgtg accaacagga atacgctgat
    35041 aaaacgccgc caggctggtt gccagcgtcg ttgttgtgtc gccgtgaacc agcacgacgt
    35101 ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg
    35161 tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt
    35221 tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa
    35281 aaggatcttt tgccaacgca tgcaccaacg gagccatctt gatggcttcc gggcgcgtac
    35341 caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc gcctttgcgc
    35401 tcgccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc
    35461 agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt
    35521 accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagagttgga
    35581 ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga
    35641 ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt
    35701 tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc
    35761 tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc ggtcgtagat ggctttcgcc
    35821 acctcttcct gacgcttcac ctgagctttc atctggatgg tgcgtgccgc ccatccgcct
    35881 ttcagctcct cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt
    35941 aacaggttat tagcgtcagg cgcggtttcg gcaataagct tcacgctgtc attgaccgcg
    36001 cgggtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca
    36061 tcggctttgc tgttgcccac catccgctgc ttgtaatagt cggtttgcag ccagaactct
    36121 ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc
    36181 gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc
    36241 tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc cgtcgcgctc
    36301 cactcttgtc gagcaaaaaa agtatacgcc agcgcgatta acgcaaacgc cagccccatg
    36361 ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca
    36421 ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt
    36481 tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa
    36541 agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa
    36601 agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg
    36661 aagcgagcag tgctgcggca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg
    36721 cacgcatgat caaatggtga atatgctgac ggtcaggaga gaatgggctc atgcctttac
    36781 gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag
    36841 cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagataa
    36901 cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca
    36961 ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga
    37021 ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacgc
    37081 aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg
    37141 cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc
    37201 caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata acaatgccaa
    37261 cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc
    37321 caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat
    37381 cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta
    37441 tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca
    37501 ctttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac
    37561 tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc
    37621 acgaagacct ttattcagaa gggaaagtcc gttccaaacc caccagtcct gtgcaaaaat
    37681 taaccatttg atatgcaaat tgcataacat gccagcaaat tccaggaatt ttcctaacat
    37741 cgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt
    37801 ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt
    37861 agtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat
    37921 gattttgcgc aggatccaca ttttctgcag ttcttcctga gtcgtgagca gctcttcttt
    37981 acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg
    38041 agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtaaataa cttcgtccat
    38101 tttagaaccg gtatcgataa gcgccgtcgc gataatggtc aggctgccgc cctcttccac
    38161 gttacgcgcc gcaccgaaga agcgtttcgg acgatgaagg gcattggcgt ccacaccacc
    38221 ggtcaacact ttacctgacg ccggaacaac agtgttgtaa gcgcgcgcca gacgagtgat
    38281 ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat
    38341 caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac
    38401 ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag
    38461 aaccatcagc acacaatccg ggtggttgta agcaatgctc tgagcaatgt tctgcagcag
    38521 catagtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg
    38581 tgatgccaga tccagtacgc gagcggttaa atcttcagta gaaccgttac cacgttccat
    38641 acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc
    38701 gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc
    38761 ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg
    38821 gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga
    38881 acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc
    38941 gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatacgagc
    39001 caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt
    39061 taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa
    39121 tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt
    39181 tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga
    39241 gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat
    39301 tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg actcacgccg
    39361 ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc caggttagcg
    39421 tcgaggaact ctttcaactg acctttagac agtgcaccca ctttggttgc cgccacttca
    39481 ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca
    39541 gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt
    39601 tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg
    39661 aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata
    39721 attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt tcgttgttgg
    39781 tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc gtaaagcaat
    39841 agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca gaagttttcc
    39901 gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt
    39961 acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt agccgggcag
    40021 gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca ttatcttctc
    40081 tctcatcctg cgattgccga tcgcaaggtg aatcaaccac gtgccttaat tatggcaccg
    40141 acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac cgctggcgga agctaccggc
    40201 ctgaagctgg gtctggctta cggtggtgat ggctacgaca aacagctgaa agtgctggaa
    40261 agcggcgttg acattctgat tggcactacg gggcgtttaa ttgactacgc caagcagaac
    40321 cacattaacc tcggtgccat tcaggtggtg gtactggacg aagccgatcg catgtacgat
    40381 ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc
    40441 ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc gttcgagcag
    40501 atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa
    40561 gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa
    40621 gaagagtggc cagacagagc gattattttc gccaatacca aataccgttg tgaagagatc
    40681 tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag
    40741 aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat tctggttgcc
    40801 accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt taactacgat
    40861 ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca
    40921 agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgag
    40981 acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt gatgaccgat
    41041 ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca atggtccgcg tcgtactggc
    41101 gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac
    41161 tgtatcaaga tggcaagaga atgaatcccg atgagcatac tgtagtaagt gattcaggtg
    41221 aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt ccacctcgtc
    41281 gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt
    41341 ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct
    41401 gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct
    41461 gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac
    41521 attacgtctt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa tcctcggttg
    41581 tccggtacag gtgatcagcg gtgaagagga agcacgtctg atttatcagg gcgttgctca
    41641 cacaactggc ggtgccgatc agcgcctggt ggtggatata ggcggtgcca gtactgaact
    41701 ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac
    41761 ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga
    41821 aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa
    41881 agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg
    41941 gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag ccattcattg
    42001 cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt tagtgttccc
    42061 gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt gtatgaccct
    42121 ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca
    42181 ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg atattgatca
    42241 ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa aggaatggca
    42301 tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct
    42361 gagcgttgac tttaaacaag cgccgcaaca cgctgcttat ctggtgcgta atctggatct
    42421 tcccggtttt acccccgcac agaaaaaatt gctggcgacg ctactgctca accagactaa
    42481 tccggtcgat ctctcatcgc tgcatcagca aaatgccgta cctccgcgcg tcgcagaaca
    42541 actctgccgt ttactccgcc tggccatcat ttttgccagc cgtcgccgtg acgatctcgt
    42601 gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg
    42661 gctaacccaa catccgctgg gtaaagagat tattgctcag gaaagccagt ggcagagcta
    42721 tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc
    42781 ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag attcgccaga
    42841 tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa
    42901 atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg caccaactcg
    42961 ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg
    43021 ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatgct gctctggtgc
    43081 ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag cccttttgac
    43141 gcgtgcagag tcatcagttg cacctgatcc agctcttctt cactctcacc acgctccatc
    43201 atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc atccagttcg
    43261 ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt
    43321 tcggcggctt tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg
    43381 atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccaac
    43441 cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc
    43501 atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccactc acccagcttt
    43561 ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg
    43621 tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat ttcaggacga
    43681 gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt
    43741 tcaaacactc gcgactgatg gttaccgcga taaagaatag cgtaatcttt gtactgcgtt
    43801 ttattgacga agtgatgcgc aatcagttca ccagtgacgc gctcggcttc atgttcttcg
    43861 ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca gttcggagaa cagacgcttc
    43921 tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa
    43981 gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg actcagcagc
    44041 accaggtttt gtggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc
    44101 acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg gctggtgttg
    44161 gtgtcctgat actcatccac cagcagatag cgaattttgt tctgccagcg ctcgcggact
    44221 tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa gtcgagaaca
    44281 ttgcacgctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acgatcccgc
    44341 tcgccctttg cctctgccgc cgcctgcgcc ggagttttga gatcgttctt ccagttggag
    44401 atggtcgaaa tcagctgttg caggagaact ttgtcatctt caatcagccc ctcggtcaac
    44461 tctttaagca aagcaagctg atcggtatcg tcaaacagcg aaaagttcgc cttcatccca
    44521 agcgccgcat actcgcgttt gatgatatcc aaccccagcg tgtgaaaagt ggagatcatc
    44581 aacccgcgcg cctctttgcg acccagcgtc tgcccgacac gctctttcat ctcgcgcgcc
    44641 gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca accgcggatc
    44701 agatgggcga ttttattggt gatcacacga gttttaccgg aacccgcgcc cgccagcacc
    44761 aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcataggt
    44821 gtattgctca atcttcgaac ggggggagga ttgtagcaga atcgcatttg aatgtgaccg
    44881 cgtgtaagcc gctaaccggt tggctgcgga tgtttaaatc agagagagaa gaccgaaatc
    44941 ggagttcatc gtgaatccat tagacttaga aaatatcggg tcgtaacgtt gtgttattac
    45001 tttttcagag tttactaagg agaggtttac tcgtggcgat taatttcagc cctaaggttg
    45061 gtgaaataca ggaatgcaac tttggtaact atcctgttat ccagaatgga cagttcagca
    45121 ccacgtatta cgatggccgc attcctcctg aaatgataaa aaacaggtta gttgtcgtgc
    45181 tcaacggaaa aattaatggc aacgcatgta tcgtcgttcc attatcaacc actcgcgatc
    45241 atgacaagtt aaatcgtgga atgcatgttg aaatagccag taatgttatc aatggcctgc
    45301 aattctttga tcagcagatt cgttgggcca aagccgacct ggtacaacaa gtcagtcgaa
    45361 accgattaaa tagagcaagg acttacagag ggtatcttaa tcaatgtttg ccacatgagt
    45421 tggttgcaga catacagagg gcggtcatca agtcaatcaa tgccatatcg ctcataaatt
    45481 gacaataaaa taaccatccc cgataatgac tttgtagccc atagaggccc taagttttct
    45541 ccccttgagg gacctgcgaa gcccggattt tccgggcttt tgccatatat gtaccagccc
    45601 ctccctgact gacaattacg ttagcatctg cggatcatgc taaaatcgcc gcctgacaat
    45661 tattcactca aggcacgatc atggcaaaaa cagcagcagc actgcatatc cttgtaaaag
    45721 aagagaaact ggctctggat cttctcgagc agattaagaa cggtgccgat ttcggcaagc
    45781 tggcgaagaa acactctatt tgcccgtcag gcaaacgcgg cggtgattta ggtgaattcc
    45841 gccagggtca gatggttccg gcgttcgata aagtggtttt ctcttgtccg gtactggagc
    45901 cgaccggccc gctgcacacc cagttcggat atcacatcat taaggtgctg taccgcaact
    45961 aatagcaagg ccttctccag gagaaggcct tgagtgtttt ctccctctcc ctgtgggaga
    46021 gggtcggggt gagggcatca gcgcgcactt aacccgcaac agcaatacgt ttcatatctg
    46081 tcatatagcc gcgcagtttc ttacctacct gctcaatcgc atggctgcga atcgcttcgt
    46141 tcacatcacg cagttgcgcg ttatctaccg cgccttccgg aatagcttta cccaagtcgc
    46201 ccggttgcag ctctgccata aacggtttca gcaacggcac acaagcgtaa gagaacagat
    46261 agttaccgta ctcagcggta tcagagataa ccacgttcat ttcgtacaga cgcttacggg
    46321 cgatggtgtt ggcaatcagc ggcagctcgt gcagtgattc ataatatgca gactcttcaa
    46381 tgatgccgga atcgaccatg gtttcgaacg ccagttcaac gcccgctttt accatcgcga
    46441 tcatcagtac gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg
    46501 cggtttcaaa cgcggttttg ccggtctctt cacgccaggt cagcagtttc ttatcatcgt
    46561 tggcccagtc cgccatcata ccggaagaga attcgccgga gatgatgtcg tccatatgtt
    46621 tctggaacag cggtgccatg atctctttca gctgttctga cagcgcataa gcacgcagtt
    46681 tcgccgggtt agagagacgg tccatcatca gggtgatgcc gccctgcttc agcgcttcgg
    46741 tgatggtttc ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt
    46801 ccaccagctt gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt
    46861 gctcacccat caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac
    46921 ggtgaccacc ggttgctgcc gcccatgctt tagcgatcgc cataccttcg cctttcggat
    46981 cgttttccgg gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac
    47041 gcacttcggt gccagggcat ttcggcgcaa ccatgactac ggtgatatct ttacggatct
    47101 gctcgcccac ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca
    47161 tcagtggctg tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca
    47221 ccagatccgc ctgcgggatc agttcttcgt aagtaccaac tttaaaaccg ttttcggtcg
    47281 ctttacgcca ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat
    47341 ccagaccaga atcacgcatg ttcaggccct ggttcagacc ctgtgcgcca cagccgacga
    47401 tgactacttt tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa
    47461 agcgacattt gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag
    47521 ccatggtgat tcctcgtgat gttgtgcttc ttattgttcg gttcgcgttt gcgagattga
    47581 attcactata tgacaggaaa tttattgcgg aaattgatat attcacaacg tcacattgca
    47641 atttttgcaa cgtcaacatc gagggctgcg ccattggatt tacgcgatct gaaaaccttc
    47701 ctgcatctgg cggaaagccg ccattttggc cgcagcgcgc gggcgatgca cgttagccca
    47761 tccacgctct cacggcagat tcagcgcctg gaagaagatc ttggtcagcc gctatttgtg
    47821 cgcgataacc gcacggtaac gctgacggaa gcgggcgaag agctgcgcgt tttcgcccag
    47881 caaacgctgt tgcagtatca gcaattgcgc cacaccatcg atcagcaagg gccgtcgctc
    47941 tctggcgaat tacatatctt ctgctcggtg accgctgcct acagccatct gccgccgatt
    48001 ctggatcgct tccgcgcgga acacccgtcg gtggagatca aactcaccac tggtgatgcg
    48061 gcagatgcaa tggaaaaagt ggtcacggga gaagcggatc tggcgattgc cgggaaaccg
    48121 gagacgctac ccggcgctgt ggcgttttcg atgctggaga atctggcagt agtgctgatt
    48181 gcgcccgcgc tgccctgccc ggtgcgtaac caggtttcgg cagagaagcc ggactggtct
    48241 acagtgccgt ttatcatggc cgatcaggga ccggtgcgtc gccgcattga actgtggttt
    48301 cgacgcaata aaatcagcaa cccgatgatt tacgccacgg ttggcgggca tgaagcgatg
    48361 gtatcgatag tggcgctcgg ctgtggtgtg gcgctgttgc cggaagtagt gctggaaaac
    48421 agccccgaac cggtgcgtaa ccgcgtgatg attttagagc gcagcgatga aaaaacgccg
    48481 tttgaactgg gcgtgtgtgc acaaaaaaag cggctgcatg agccgctgat tgaggcgttc
    48541 tggaggattt tgccgaacca caaatgacgt tgtcgcgcgg gtaggcctga taagcgaagc
    48601 gctatcaggc atttttccct aacccgccaa aaagaacctg aacgccgggt tattggtttc
    48661 gtcgtggcaa tcgtagccca gctcattcag ccgggtttcg aaatccggtt catggtcgcc
    48721 aagttcgaac gccgccagta cgcgcccgta gtcggtgcca tggctgcgat agtggaacaa
    48781 agaaatgttc cagtgcgtac ccagcgtgtt gaggaagcgc agcagcgcgc ccggtgattc
    48841 cgggaattcg aagctgtaga ggcgttcctg caacggatgt gacggacgcc cgccgaccat
    48901 atagcgcaca tgcaacttcg ccatttcgtc gtcggagaga tccaccacgc tgtagccgcc
    48961 gtcgttgagc atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc
    49021 gacaaagatg caggcgtttt tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg
    49081 cccgccaagc agttggcaga atttgaggaa gctgcctttt tcttccggaa tggtcaccgc
    49141 caacaacgct tcacgctgtt cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg
    49201 gaagttcacg ttggcaccgg aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg
    49261 ggcgatatat tttttcattc ccgccagcgc cagcgcgcca gagggttccg ccaccgcgcg
    49321 cacatcttcg aataaatcct tcatcgccgc acagatcgca tcactatcga cggtgatgat
    49381 gtcgtcgaga tactcctggc ataaacggaa ggtttcgtca ccgatgcgtt ttaccgctac
    49441 gccttcagca aatagcccta cgcgcggcag atcaaccgga tgacccgcat ccagcgctgc
    49501 tttcaggcag gcggagtctt ccgcttctac ggcgatcact ttgatttgtg gcatcagttg
    49561 tttgatcagc accgcaacgc ccgccgccag accgccgccg ccgactggca caaatacgcg
    49621 gtcgagatgg gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc cggcaatcac
    49681 catcggatgg tcgaacggcg gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc
    49741 tttagctttc gcttcgtcaa agttcgcgcc atgaagcagc acttcgccgc cgaatccacg
    49801 taccgcatcg actttgatgt cggcggtggc ggttggcata acgatcaggg ccttcacgcc
    49861 taaccgcgca gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag cagtgatcac
    49921 gccgtgcgct ttttgttctt ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt
    49981 aaagctgtgc actggctggc gatcttcgcg cttcaccaga atgacgttat caagacgcga
    50041 cgacagtttt tccatttttt gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg
    50101 cagcactgct cttaaatatt cggcaccttc cggagcaccg gacaggggtt gcgagtcagc
    50161 cattattaac cccccagttt cgatttatcg cgcaccgcac ctttgtcggc gctggttgcc
    50221 aggctggcgt aggcgcgcag ggcaaaggaa acctgacgtt cacggttttt cggcgtccag
    50281 gctttgtcac ctcgagcgtc ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc
    50341 tgtaactgaa tgccacggtt cgggatgtct atagcgatca ggtcaccatc ttcaatcagg
    50401 ccaatgctgc cgccgcttgc cgcttccggt gagacgtggc cgatggaaag accagaggtg
    50461 ccgccagaga aacgaccgtc ggtgatcagc gcacaggctt taccgagacc cattgatttc
    50521 aggaagctgg ttgggtagag catttcctgc atgccagggc cgcctttcgg gccttcataa
    50581 cggatcacca ccacatcgcc cgcgacaact ttgccaccga ggatcgcttc taccgcgtcg
    50641 tcctggcttt cgtacacttt cgccgggccg gtgaatttga ggatgctgtc atcgacgccc
    50701 gcggttttaa cgatgcagcc gttttctgcg aaattaccgt agagcaccgc caggccgccg
    50761 tctttgctgt aggcgtgttc cagcgagcgg atacagccat tggcgcgatc gtcatcgaga
    50821 gaatcccaac ggcaatcctg cgagaatgcc tgtgtggtac gaatgcctgc aggacctgcg
    50881 cggaacatat tttttaccgc gtcatcctgg gtcagcataa cgtcgtattg ttccagcgtt
    50941 tgcggcaacg tcaggccaag tacgtttttc acatcacggt tcagtaaccc cgcgcgatcc
    51001 agttcgccga gaataccgat aacaccacca gcacggtgaa catcttccat atggtatttc
    51061 tgggtgctcg gcgcaacttt acacagctgt ggaaccttgc gggaaagctt atcgatatca
    51121 ctcatggtga agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag tacggtgtta
    51181 gtcgatccac ccatcgcgat atccagcgtc atggcgtttt caaacgccgc tttgctagca
    51241 atattgcgcg gcagtgcact ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca
    51301 atgcgtttac cggcattgag gaacaactgc ttacggtcgg cgtgggttgc cagcagcgag
    51361 ccgttgcccg gctgcgacag acccagcgct tcggtcaggc agttcattga gttagcggta
    51421 aacatcccgg agcaggagcc gcaggtcgga cacgcggaac gttcaacctg atcgctctgg
    51481 gagtcagaaa ctttcgggtc tgcgccctgg atcatcgcat caaccagatc gagtttgatt
    51541 atctgatcgg acagtttggt tttcccggcc tccatcgggc cgccggaaac aaagatcacc
    51601 ggaatattca ggcgcaggga agccatcagc atccccgggg tgattttgtc gcagttagag
    51661 atgcagacca tggcgtcggc gcagtgggcg ttgaccatat actcaacgga atcagcgatc
    51721 agttcgcgag atggcagtga ataaagcatc cccccgtggc ccatggcaat cccatcatcc
    51781 accgcaatgg tgttgaactc tttggcaacg ccgccagccg cttcaatttg ttcggcgacc
    51841 agtttaccga gatcgcgcag atggacgtga cccggtacaa attgggtgaa cgagttcaca
    51901 accgcgataa tcggcttgcc aaaatcggcg tcggtcattc cggtggcgcg ccacagcgca
    51961 cgagcacccg ccatattacg accatgagtg gtggtggcgg aacggtactt aggcatactt
    52021 tatttactcc cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat
    52081 ttattgatta acttgatcta accagcccca tttatcttcg gtttcgccag tgaagaggcc
    52141 gaagaaggct tgctgaatgc gtttggtaac cgggccacaa cggccttcgc caacctgaat
    52201 accatctaca ctgcgcactg gcgtgatttc tgccgcagta ccggacataa acacttcatc
    52261 cgccaggtac agggattcgc gcgacagaac ctgctcacgc acttcaattc ccagctcttt
    52321 cgccagtttg atgatggcat cacgggtaat acccggcagc gcggaggagg tgaacggtgg
    52381 ggtgaacagc acaccatctt tcacttcaaa caggttttcg cctgcgcctt cagagatata
    52441 accgttcaca tccagcgcga taccttcctg ataaccgtgg cggcgcgctt cgctacccac
    52501 cagcagggaa gagagatagt taccaccggc ttttgccgcg gttgggatgg tgtttggtgc
    52561 tgcgcggttc caggaggaaa ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag
    52621 atacgctccc cacgggaaag cggcgataat cacatcggtt gagtatcccg ctggcgggtt
    52681 tacacccatg ccaacatcac caacgaagat cagcggacgg atataggcgc tggtgagatt
    52741 gtttttgcgg atcacgtcac gacaagcttc catcagctca tcaatgctct gcgaaaccgg
    52801 gaagcgatag attttggcgg agtcatgcag acgctgcata tgctcacgat ggcggaatac
    52861 aaccggtcct ttgtgcgagt cgtagcaacg gatgccttca aaaaccgaag tgccatagtg
    52921 cagcgcgtgc gacatcacat gcaccttagc gtcttcccag cgaaccatct ccccattgaa
    52981 ccaaatgtaa tcagctttct tcgtggtcat ttttatattc cttttgcgct caggcgcgga
    53041 tttgttgtga tgtggttgtg ctctggcaga tggcaacgtg tgcgacgtcc accagtttat
    53101 ttaactgact aaacagtaag tcgaccgacc gtgggctggc aacggtcaat tcgatattta
    53161 tattttgtgc atcgctggcg gcagccatat tcattgagca gacgtggaaa ccacgatgac
    53221 gcaccacgcg taaaacacgt tctaaggttt ccggattgaa gcgagccgat acattgacct
    53281 gatgttgcat catgataatt tctccaacat ttctgaatta ctggcgccag gcggcaccag
    53341 cggccagacg ttctcaagtt cgtcgattga gacatgaagc aggtatggcc catcactgtt
    53401 cagcatggtg tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt ggccagggat
    53461 gccgaaggcg ctggctaaca tgaggaaatc ggggttatca gtaagggtgg tttcgctgta
    53521 tcgttcctga aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt ggttatcgag
    53581 taagacgatt ttcaacggta actgcttgcg ttttacggtg cccagctctt gcacattcat
    53641 catgaaagag ccgtcaccgg agatacagac gacagtatcg ttcggtcgtg cgacttgtgc
    53701 gccaaccgcc gctggtaaac cgaaacccat ggtgcctaag ccgctggagg taatgaaatt
    53761 ttccgggcga gtgtgtgcga tgtgctgcgc ggcccacatc tggtgctgcc ccacatctgt
    53821 ggtcacgacg caatccgcag gtttacgatc cgacagttgt tttaacaaca acggcgcgta
    53881 gatagcgtca ccgggatggt cgtaacgcca ggtatgttca tcacgcagct gcgcgcagta
    53941 ttgctgccag tcattgatat ttaacggctg ctgtaatgct ggtaacagag catttaaatc
    54001 accttgtaat gccacatgtg cctgacgcag cttgttcatt tctgccgggt cgatatccat
    54061 atggataaca ctggcgtgtg gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa
    54121 acgtgcaccc acggcgatca gcaagtcgca ctcctgcacc gcgaagttcg ccgctttggt
    54181 gccatgcatt cccagcatgc ccaggtagta cggataatct gcttcaactg cgcccagccc
    54241 tttcagcgtg caggtggcag gcatttttgt ggtagcgaga aattctcgta aagcaggaac
    54301 tgcctgcgcc atacccacgc caccaccaac gtacagcatc ggtttttgcg cttttgccag
    54361 catctggcgc gcttgctcaa cttcggcatg tgggaaagtc acttcgtttt caacggtggt
    54421 gaaccacggt tccaggtcac cgctggctag ctggatatct tttgggatat cgaccagaac
    54481 cggaccagga cgacctgagc tggcaacgtc gaatgcttca gccataatgc gcggcaactc
    54541 ttccagcgac tgcaccagaa agctgtgctt ggtacaggct aacgacaatc ccaggacatc
    54601 cacttcctga aatgcgtccg tgccgataaa cggtgcggac acttgaccgg tgatggcaac
    54661 aacagggata gaatctaaca gtgcgtccgc aagcccggtt atcaggttgg ttgcgcccgg
    54721 accagacgtg gcgatacata cgccagtttt gccggtagca cgggcataac cgatagccgc
    54781 cattgccgca ccctgctcat gtcggcacag caagtgctcc acgccgccgt catacaatgc
    54841 atcgtaaacc ggcataattg cgccacccgg ataaccgaaa acggtgttca caccctgtgc
    54901 ccgcaacgca tgtaccaccc actgtgcgcc attcatagtt agttccccgt cctgaatctt
    54961 gagaaacaga attttgtgct gttattcatt gtctgctcct cggttatatt tttaaggtca
    55021 aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag
    55081 cctttcctcg tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca
    55141 cgaccaggct aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga
    55201 aggaatgcct aaagagttac catagatttt gcgaatagca caagatattt tttggccgat
    55261 ttttatgatg cgtttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga
    55321 gtgaaaaaaa ttcaatttca gtatgaatgt tgttcccttc ctcgtagttc atcctttgtc
    55381 cgctgcattt ttgcgagcat cattccaccg ccagaacaac tcaacttcac ttgcacaaat
    55441 aaacaggaat cagccactaa aattcacgaa ttagcgggct acagcagcga tatcttatgc
    55501 acataatagt gactcaagga gggcttatgt cactgtcaat tgttcatacc cgcgcagccc
    55561 tgggagtaaa tgcgccccca atcactgttg aggtacatat cagtaaaggt ctacccggct
    55621 taacgatggt gggcttacca gaaacaactg taaaagaagc tcgcgatcgc gtgcgcagcg
    55681 ccattatcaa tagcggatat gaatatccgg cgaaaaaaat caccatcaac ctggctccag
    55741 ccgatctgcc aaaagaaggg ggacgatatg atttacctat cgccattgcg ttgctggcgg
    55801 cctcagaaca gcttacagcc aataagttag atgaatatga attagtcgga gaactggcgc
    55861 ttacaggcgc tctgcgtggc gttcccggcg caatctccag tgcaactgaa gctattaagt
    55921 cgggcagaaa aattatcgtc gcgaaagata acgaagatga agtggggcta attaacggtg
    55981 aaggatgcct gatagccgat catctgcagg ctgtctgtgc gtttctggaa ggtaagcacg
    56041 ctctcgaacg cccgaaacca actgatgcag tatcccgggc gctacaacat gatctcagtg
    56101 atgttgtcgg tcaggagcaa ggaaagcgag gactggaaat taccgccgct ggggggcaca
    56161 accttttact gattgggccg ccgggaacag gtaaaacaat gctcgccagc cgtattaatg
    56221 gcctgttgcc agatttaagc aatgaagagg cactggagag cgctgcgata ttaagtctgg
    56281 taaatgctga atcagtacaa aaacaatggc ggcagcgccc gttccgctca ccgcatcaca
    56341 gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt
    56401 tggcgcataa cggcgtgctt tttcttgatg agctacctga atttgaacgg cgtacactgg
    56461 atgccttgcg agagccgatt gaatccgggc agatccatct ttcacgcaca cgagccaaaa
    56521 taacctatcc agcccgtttc cagcttgttg cggcgatgaa tcccagccct accggacatt
    56581 atcagggaaa ccataaccgc tgcacgccag aacagacatt acgttatctc aaccggctct
    56641 cggggccctt tctcgaccgc ttcgatctct cactggagat cccattacca ccccccggca
    56701 ttttgagtaa aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg
    56761 ccgccagaga gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg
    56821 aaatacgcca attctgcaaa cttgagagcg aagatgcgat gtggttggaa ggaacactga
    56881 tccatctggg gttatcgatt cgtgcctggc agcggttatt gaaagttgca cgaaccattg
    56941 ctgatattga tcagtctgac attatcacac gtcagcattt gcaggaggca gttagctatc
    57001 gtgcgattga ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt
    57061 cgcccttttt attaatcatc agaatcggtg tagtcttcag caccttcaac ctgcggttta
    57121 ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac
    57181 acgcgttctg cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc
    57241 tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga
    57301 cgctcaagca gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga
    57361 taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct
    57421 cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta
    57481 aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa
    57541 ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg
    57601 ctctatctga cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt
    57661 gtgaaccttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta
    57721 ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgcaagga ggtggcgcat
    57781 acctcacgac ataacgagtt ttctatcggt gccagcgcct cattgtggga atgtatgctt
    57841 aatcagtggc tgggacgttt gtatcaaaat caggatgccc atacaggctt acagttcgaa
    57901 gcgcgaattg cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt
    57961 cttattacca ctgaagcgcc caaaatggac gaatttagta gtcagttgct gggatatttc
    58021 actttagcgc tttataccag tgccccttca aaactaaagg gagatcttaa ttatctgcga
    58081 cttgagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtg
    58141 cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt
    58201 tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat
    58261 agcacaacac tttcacggcc gctttatgcc atatggctgc aaaatagcga taaaaatacg
    58321 ttgattcgcg atcttttgaa aattaacgtg ctggatgaag tgtattaata tgaatggctg
    58381 gcaaggatgc cggtagaagg atttacttcg gagagggtta tttcagataa aaaaaatcct
    58441 tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg
    58501 ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg cggaacggac
    58561 gggactcgaa cccgcgaccc cctg
//
LOCUS       DAZUFB010000017        57922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZUFB010000017 DAZUFB010000000
VERSION     DAZUFB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.017"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHY3M_001243"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHY3M_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFS4316189.1"
                     /translation="MINSINSFFSSIPRSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHY3M_001244"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHY3M_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHY3M_001245"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHY3M_001245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHY3M_001246"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHY3M_001246"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFS4316190.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHY3M_001247"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHY3M_001247"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFS4316191.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHY3M_001248"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHY3M_001248"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFS4316192.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHY3M_001249"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHY3M_001249"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFS4316193.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHY3M_001250"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHY3M_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFS4316194.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHY3M_001251"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHY3M_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFS4316195.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8873
                     /gene="appC"
                     /locus_tag="ACHY3M_001252"
     CDS             7329..8873
                     /gene="appC"
                     /locus_tag="ACHY3M_001252"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFS4316196.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8885..10021
                     /gene="appB"
                     /locus_tag="ACHY3M_001253"
     CDS             8885..10021
                     /gene="appB"
                     /locus_tag="ACHY3M_001253"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS4316197.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHY3M_001254"
     CDS             10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHY3M_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFS4316198.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10206..11504
                     /gene="appA"
                     /locus_tag="ACHY3M_001255"
     CDS             10206..11504
                     /gene="appA"
                     /locus_tag="ACHY3M_001255"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFS4316199.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11619..13799)
                     /gene="etk"
                     /locus_tag="ACHY3M_001256"
     CDS             complement(11619..13799)
                     /gene="etk"
                     /locus_tag="ACHY3M_001256"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFS4316200.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13819..14265)
                     /gene="etp"
                     /locus_tag="ACHY3M_001257"
                     /gene_synonym="yccY"
     CDS             complement(13819..14265)
                     /gene="etp"
                     /locus_tag="ACHY3M_001257"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFS4316201.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14253..15392)
                     /gene="gfcE"
                     /locus_tag="ACHY3M_001258"
     CDS             complement(14253..15392)
                     /gene="gfcE"
                     /locus_tag="ACHY3M_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFS4316202.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15438..17534)
                     /gene="gfcD"
                     /locus_tag="ACHY3M_001259"
     CDS             complement(15438..17534)
                     /gene="gfcD"
                     /locus_tag="ACHY3M_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS4316203.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17534..18280)
                     /gene="gfcC"
                     /locus_tag="ACHY3M_001260"
     CDS             complement(17534..18280)
                     /gene="gfcC"
                     /locus_tag="ACHY3M_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS4316204.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18277..18921)
                     /gene="gfcB"
                     /locus_tag="ACHY3M_001261"
     CDS             complement(18277..18921)
                     /gene="gfcB"
                     /locus_tag="ACHY3M_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFS4316205.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19028..19333)
                     /gene="gfcA"
                     /locus_tag="ACHY3M_001262"
     CDS             complement(19028..19333)
                     /gene="gfcA"
                     /locus_tag="ACHY3M_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFS4316206.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19775..19987)
                     /gene="cspH"
                     /locus_tag="ACHY3M_001263"
     CDS             complement(19775..19987)
                     /gene="cspH"
                     /locus_tag="ACHY3M_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFS4316207.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20273..20485
                     /gene="cspG"
                     /locus_tag="ACHY3M_001264"
     CDS             20273..20485
                     /gene="cspG"
                     /locus_tag="ACHY3M_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFS4316208.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20496..20684
                     /gene="ymcF"
                     /locus_tag="ACHY3M_001265"
     CDS             20496..20684
                     /gene="ymcF"
                     /locus_tag="ACHY3M_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4316209.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20659..20889
                     /gene="ymcE"
                     /locus_tag="ACHY3M_001266"
     CDS             20659..20889
                     /gene="ymcE"
                     /locus_tag="ACHY3M_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFS4316210.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20879..21052
                     /gene="gnsA"
                     /locus_tag="ACHY3M_001267"
     CDS             20879..21052
                     /gene="gnsA"
                     /locus_tag="ACHY3M_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFS4316211.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21100..22173)
                     /gene="yccM"
                     /locus_tag="ACHY3M_001268"
     CDS             complement(21100..22173)
                     /gene="yccM"
                     /locus_tag="ACHY3M_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4316212.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(22245..24989)
                     /gene="torS"
                     /locus_tag="ACHY3M_001269"
     CDS             complement(22245..24989)
                     /gene="torS"
                     /locus_tag="ACHY3M_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFS4316213.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEKQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQSLETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            25072..26100
                     /gene="torT"
                     /locus_tag="ACHY3M_001270"
     CDS             25072..26100
                     /gene="torT"
                     /locus_tag="ACHY3M_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFS4316214.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26073..26765)
                     /gene="torR"
                     /locus_tag="ACHY3M_001271"
     CDS             complement(26073..26765)
                     /gene="torR"
                     /locus_tag="ACHY3M_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFS4316215.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26895..28067
                     /gene="torC"
                     /locus_tag="ACHY3M_001272"
     CDS             26895..28067
                     /gene="torC"
                     /locus_tag="ACHY3M_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFS4316216.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28067..30613
                     /gene="torA"
                     /locus_tag="ACHY3M_001273"
     CDS             28067..30613
                     /gene="torA"
                     /locus_tag="ACHY3M_001273"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFS4316217.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30610..31209
                     /gene="torD"
                     /locus_tag="ACHY3M_001274"
     CDS             30610..31209
                     /gene="torD"
                     /locus_tag="ACHY3M_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFS4316218.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(31302..31607)
                     /gene="cbpM"
                     /locus_tag="ACHY3M_001275"
     CDS             complement(31302..31607)
                     /gene="cbpM"
                     /locus_tag="ACHY3M_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFS4316219.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31607..32527)
                     /gene="cbpA"
                     /locus_tag="ACHY3M_001276"
     CDS             complement(31607..32527)
                     /gene="cbpA"
                     /locus_tag="ACHY3M_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFS4316220.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32787..34046
                     /locus_tag="ACHY3M_001277"
     CDS             32787..34046
                     /locus_tag="ACHY3M_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFS4316221.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEYEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            34338..35579
                     /gene="agp"
                     /locus_tag="ACHY3M_001278"
     CDS             34338..35579
                     /gene="agp"
                     /locus_tag="ACHY3M_001278"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFS4316222.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(35617..35844)
                     /gene="yccJ"
                     /locus_tag="ACHY3M_001279"
     CDS             complement(35617..35844)
                     /gene="yccJ"
                     /locus_tag="ACHY3M_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFS4316223.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(35865..36461)
                     /gene="wrbA"
                     /locus_tag="ACHY3M_001280"
     CDS             complement(35865..36461)
                     /gene="wrbA"
                     /locus_tag="ACHY3M_001280"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS4316224.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            36833..37006
                     /gene="ymdF"
                     /locus_tag="ACHY3M_001281"
     CDS             36833..37006
                     /gene="ymdF"
                     /locus_tag="ACHY3M_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS4316225.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(37089..38417)
                     /gene="rutG"
                     /locus_tag="ACHY3M_001282"
     CDS             complement(37089..38417)
                     /gene="rutG"
                     /locus_tag="ACHY3M_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFS4316226.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(38438..38932)
                     /gene="rutF"
                     /locus_tag="ACHY3M_001283"
     CDS             complement(38438..38932)
                     /gene="rutF"
                     /locus_tag="ACHY3M_001283"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFS4316227.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(38943..39533)
                     /gene="rutE"
                     /locus_tag="ACHY3M_001284"
     CDS             complement(38943..39533)
                     /gene="rutE"
                     /locus_tag="ACHY3M_001284"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFS4316228.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(39543..40343)
                     /gene="rutD"
                     /locus_tag="ACHY3M_001285"
     CDS             complement(39543..40343)
                     /gene="rutD"
                     /locus_tag="ACHY3M_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFS4316229.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(40351..40737)
                     /gene="rutC"
                     /locus_tag="ACHY3M_001286"
     CDS             complement(40351..40737)
                     /gene="rutC"
                     /locus_tag="ACHY3M_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFS4316230.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(40749..41441)
                     /gene="rutB"
                     /locus_tag="ACHY3M_001287"
     CDS             complement(40749..41441)
                     /gene="rutB"
                     /locus_tag="ACHY3M_001287"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFS4316231.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(41441..42532)
                     /gene="rutA"
                     /locus_tag="ACHY3M_001288"
     CDS             complement(41441..42532)
                     /gene="rutA"
                     /locus_tag="ACHY3M_001288"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFS4316232.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            42820..43458
                     /gene="rutR"
                     /locus_tag="ACHY3M_001289"
     CDS             42820..43458
                     /gene="rutR"
                     /locus_tag="ACHY3M_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFS4316233.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(43498..47460)
                     /gene="putA"
                     /locus_tag="ACHY3M_001290"
     CDS             complement(43498..47460)
                     /gene="putA"
                     /locus_tag="ACHY3M_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4316234.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            47515..47724
                     /locus_tag="ACHY3M_001291"
     CDS             47515..47724
                     /locus_tag="ACHY3M_001291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316235.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            47883..49391
                     /gene="putP"
                     /locus_tag="ACHY3M_001292"
     CDS             47883..49391
                     /gene="putP"
                     /locus_tag="ACHY3M_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFS4316236.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            50057..50887
                     /gene="efeU"
                     /locus_tag="ACHY3M_001293"
     CDS             50057..50887
                     /gene="efeU"
                     /locus_tag="ACHY3M_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFS4316237.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            50945..52072
                     /gene="efeO"
                     /locus_tag="ACHY3M_001294"
     CDS             50945..52072
                     /gene="efeO"
                     /locus_tag="ACHY3M_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFS4316238.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            52078..53349
                     /gene="efeB"
                     /locus_tag="ACHY3M_001295"
     CDS             52078..53349
                     /gene="efeB"
                     /locus_tag="ACHY3M_001295"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFS4316239.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQDEKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            53968..54756
                     /gene="phoH"
                     /locus_tag="ACHY3M_001296"
     CDS             53968..54756
                     /gene="phoH"
                     /locus_tag="ACHY3M_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFS4316240.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            55337..56023
                     /locus_tag="ACHY3M_001297"
     CDS             55337..56023
                     /locus_tag="ACHY3M_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFS4316241.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(56114..56249)
                     /locus_tag="ACHY3M_001298"
     ncRNA           complement(56114..56249)
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_001298"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            56468..57670
                     /locus_tag="ACHY3M_001299"
     CDS             56468..57670
                     /locus_tag="ACHY3M_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4316242.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT        13937 a        14523 c        15250 g        14212 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttac gcgtaacact tgatatactg
      241 cgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gccaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacgca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgtgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt
     1801 gtagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccccgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcgaa ggacgtcatc ctttccctga
     1981 tttccctcga ttatgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 tcgaagacat catcacgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcggccc aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccgctga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcttccac acgctggaat gatggcgttt
     2581 ctttcccaat ccagtctggt cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaacc gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcatcc tacaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgctatcggt attaaagtgc cggacaacgc aaatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctt gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcttggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 actgccgcca gaagctaacc tgatgggctt tgcccactat ctcgaagctc tcgatttcca
     3481 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     3541 tgtcggcggg atgccttgcg ccatcaacat tgacgaaagc ggcgcggtcg gggcagtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcaat tatcacccgc acggcggact tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg
     3721 cactggtctt tctgataaat gcgttctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgatat cccaacgatc aggtcgggcg tcatccgttc gatggcatca ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccacgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcgcgcacg ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgttgaacc tgccgctttc cggtatccag tcaacgttag gccgcatttt
     4201 gtgccgcgcg cacgaagcgc agtgggccgc aggtaagttg cagtatttct tcgacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacctggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cggcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgacccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 tgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtccg catctggcac tggttgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg gatactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatggt
     4861 ttttaccgtg gttttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat ataggccata atcccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggttttgc
     5101 gctgtatagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg atgggtggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gctgattggc gcgtttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcatcaga acagtttctc cgaagtgttg gcgctggcgg atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggtagcctg
     5761 agcgaactgg cacgggagca actgcccgct gcggaacagg cggcgctggc gcagctggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg gatgctggcg cgcggctgga
     6001 cgccagtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtgctgt
     6061 tattaagcag tgacccgaaa cgcacgccag aggtcagcga taatccggta atgattggcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc tgccacttta gtgtttaccg
     6241 gcggaaacta tcgcggcgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaacat ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccaac cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagt cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg accgcgctgt
     7381 atcacttttt atttgtaccc cttaccctgg ggctgatttt tttgctggct atcatggaaa
     7441 ccatttacgt ggtcaccggc aaaacaatct accgcgatat gacgcgcttc tggggtaagc
     7501 tcttcggtat caattttgct cttggcgtgg ctaccggcct gaccatggag tttcagtttg
     7561 gtactaactg gtcattctat tccaactatg tgggcgatat ttttggcgca ccactggcga
     7621 tggaagcatt aatggccttc ttcctcgaat ccacctttgt cgggctgttc ttcttcggct
     7681 ggcaacggct gaataaatac cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa
     7741 atctctctgc gttgtggata ttgaatgcca acggttggat gcaatacccg accggtgcgc
     7801 attttgatat cgacaccctg cgtatggaga tgaccagctt cagcgagctg gtctttaacc
     7861 cggtcagcca ggtgaaattt gtgcacaccg taatggcggg ctacgtgacc ggggccatgt
     7921 ttattatggc gatcagcgcc tggtatttac tgcgcggacg ggagcgcgat gtcgcattac
     7981 gttcgtttgc catcggttcc gtcttcggta ctctggcgat tatcggtacc ctgcaactcg
     8041 gagacagttc tgcgtatgaa gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag
     8101 gggagtggca aacggaacct gcacctgcac cgttccatgt ggttgcctgg ccggaacagg
     8161 atcaagagcg taacgccttt gccatcaaaa ttcccgcgct gctagggatc ctcgccactc
     8221 actcattaga taaacccgtg ccgggtctga agaatttgat ggctgaaacc tacccacgct
     8281 tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc
     8341 cgcatgtgtt gcaggcattc cgggaactgg aaggtgacct gggctacggc atgttgctct
     8401 cccgctatgc gccggatatg aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg
     8461 gcgcgatacc tcaggttgcg ccggtattct ggagtttccg catcatggtg ggctgtggtt
     8521 ccctgctgct actggtgatg ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc
     8581 agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc gttgccgtgg attgcgattg
     8641 aagccgggtg gtttatgacc gagtttggtc gtcagccgtg ggcgatacag gacatcttac
     8701 cgacatactc cgcgcactcc gctttaacca caggacaact ggctttctca ctgatcatga
     8761 tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc
     8821 gtctggggcc gagcgcgatg cagagtgaac aaccgacgca gcaacagggg taaaggagaa
     8881 aatcatgttt gattatgaaa cattgcgctt catctggtgg ctgctgattg gcgtgatcct
     8941 ggtggtcttt atgatctccg acggatttga catggggatc ggctgtctgc tgccgctggt
     9001 ggcgcgtaat gatgatgaac gccggatagt gataaacagc gttggtgcac actgggaagg
     9061 caaccaggtc tggttgatcc tcgctggtgg ggcattattt gccgcctggc ccagagtgta
     9121 tgcagcggcg ttttccggct tttatgtggc gatgatcctg gtgctgtgct cactgttctt
     9181 ccgcccgctg gcctttgatt atcgcggaaa aatcgccgat gcacgctggc gtaaaatgtg
     9241 ggacgccggt ctggtcatcg gcagtctggt gccgccggta gtcttcggta tcgccttcgg
     9301 caacttgttg ctcggcgtgc cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg
     9361 cagcttctgg caactgctga cgccattccc tttattgtgc ggattgctca gccttgggat
     9421 ggtgattttg caaggtggcg tctggttaca actgaaaact gttggtgtga ttcatctgcg
     9481 ttcacagctg gccaccaaac gcgctgcact gttggtgatg ctgtgctttt tgctggcggg
     9541 ttactggctg tgggtcggta ttgatggctt tgtactgctc gcccaggatg ctaacggtcc
     9601 ttccaatccg ttaatgaaac tggtggcagt gctacctggt gcctggatga ataatttcgt
     9661 cgaatcgccc gttttgtgga tcttcccgct gctggggttc ttctgcccat tgctgacggt
     9721 gatggcgatt tatcgtggtc gcccgggttg gggattttta atggcgtcat tgatgcaatt
     9781 tggcgtgatt ttcacggcag gcatcacgct gttccccttt gtcatgccgt caagcgtgag
     9841 tccaatctcc agcctgacgt tgtgggacag cacttccagt cagctgacgc tgagcattat
     9901 gttggtaatc gtgctgatat ttttgcccat tgtgttgctc tacactctct ggagctacta
     9961 caaaatgtgg ggacgcatga caacagaaac tctccgccgt aacgaaaacg agttgtacta
    10021 aggagcagga acaatgtggt atttactttg gttcgtcggc attttgttga tgtgttcgct
    10081 ctccaccctt gtgttggtat ggctggaccc gcgtctgaaa agttaacgaa cgtaggcctg
    10141 atgcggcgca ttagcatcgc atcaggcaat caataatgtc agatatgaaa agcggaaaca
    10201 tatcgatgaa agcgatctta atcccatttt tatctcttct gattccgtta accccgcaat
    10261 ctgcattcgc tcagagtgag ccggagctga agctggaaag tgtggtgatt gtcagtcgtc
    10321 atggtgtgcg tgctccaacc aaggccacgc aactgatgca ggatgtcacc ccagacgcat
    10381 ggccaacctg gccggtaaaa ctgggttggc tgacaccgcg cggtggtgag ctaatcgcct
    10441 atctcggaca ttaccaacgc cagcgtctgg tagccgacgg attgctggcg aaaaagggct
    10501 gcccgcagtc tggtcaggtc gcgattattg ctgatgtcga cgagcgtacc cgtaaaacag
    10561 gcgaagcctt cgccgccggg ctggcacctg actgtgcaat aaccgtacat acccaggcag
    10621 atacgtccag tcccgatccg ttatttaatc ctctaaaaac tggcgtttgc caactggata
    10681 atgcgaacgt gactgacgcg atcctcagca gggcaggagg gtcaattgct gactttaccg
    10741 ggcatcggca aacggcgttt cgcgaactgg aacgggtgct taattttccg caatcaaact
    10801 tgtgccttaa acgtgagaaa caggacgaaa gctgttcatt aacgcaggca ttaccatcgg
    10861 aactcaaggt gagcgccgac aatgtctcat taaccggtgc ggtaagcctc gcatcaatgc
    10921 tgacggagat atttctcctg caacaagcac agggaatgcc ggagccgggg tggggaagga
    10981 tcaccgattc acaccagtgg aacaccttgc taagtttgca taacgcgcaa ttttatttgc
    11041 tacaacgcac gccagaggtt gcccgcagcc gcgccacccc gttattagat ttgatcaaga
    11101 cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg tgtgacatta cccacttcag
    11161 tgctgtttat cgccggacac gatactaatc tggcaaatct cggcggcgca ctggagctca
    11221 actggacgct tcccggtcag ccggataaca cgccgccagg tggtgaactg gtgtttgaac
    11281 gctggcgtcg gctaagcgat aacagccagt ggattcaggt ttcgctggtc ttccagactt
    11341 tacagcagat gcgtgataaa acgccgctgt cattaaatac gccgcccgga gaggtgaaac
    11401 tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat gtgttcgttg gcaggtttta
    11461 cgcaaatcgt gaatgaagca cgcataccgg cgtgcagttt gtaatgcata aaaaagagca
    11521 ttcagttacc tgaatgctct gaggctgatg acaaacgaag aactgtctaa tgcgtagacc
    11581 ggaaaaggcg ttcacgccgc atccggccac tttcagtttt actctttctc ggagtaacta
    11641 taaccgtaat agttatagcc gtaactgtaa gcggtgctgg cgcgtttaat cacaccattg
    11701 aggatagcgc ctttaatatt gacgcctgcc tgttccagac gctgcattga caaactcacc
    11761 tctttggcgg tgttcaagcc aaaacgcgca accagcaggc tggtgccaac agaacgcccc
    11821 acgaccgcgg catcactcac cgccagcatc ggcggcgtat cgacaatcac cagatcgtaa
    11881 tggtcgttcg cccattccag taattgacgc atccgatcgc gcatcagcag ttcagacggg
    11941 ttaggtggca cctgaccgcg agtaatcaca tcaaagcctc ctttgccaaa atgctggatc
    12001 actttgttga gctcatcttt acctgccaga tattccgaca agccatgttc attactcacg
    12061 gtaaacaggt tatgcgaata accacggcgt aagtcggcat caataaataa cactttttga
    12121 tcggactggg cgatcaccgc tgccagagtt gaactgacaa acgttttacc actgtctggc
    12181 gtcgcaccgg tgatcatcag aatgttattc tccgtctcca tcatagcgaa atgcagactg
    12241 gttcgtagcg cacgtacggc ttccacagca gaatccgccg ggttatccac cgccaggaag
    12301 gggatatttt tagtacgatg gcgctgctga ttagaaaata aatttttctt acgcagacgg
    12361 gtgcgtttat ccagccactc ggacattggg atagtggcat aaacgctgat gccgtgctct
    12421 tccagttgtt ccggggcttc tacaccacga cgcaacatcg cacgcgccag cacggcaccc
    12481 acagaaataa acaggccaag aataaaacca agcaccacat tcaacgcttt tttcggtttc
    12541 actggctgcg gctgagtgac tgccgggtcg ataatccgca cgttaccaat ggcactggat
    12601 ttcgaaatac tcaactcctg ctggcggtta agtaattgca gatataccgc acggcccgct
    12661 tctacgtcac gacttaaacg caacacttcc tgttgggtgg aaggcattgc cgatacccgc
    12721 ttattcaggc gtttgcgttc ttgctccagc gtctggcgtt tttccagcag cgcacgataa
    12781 gttgggtgat ctttcttata cagctgggag atctctgcct cgcggaaggt cagctcattg
    12841 agttgattat caacgttcac aatctgctca agaacggctt tggcttccag gttaaggtca
    12901 accgaatcgc gctgctggcg ataaacgttg agtttttctt ccgcctggtc cagctcgctg
    12961 cgcacttcag gtaactggcg ctgtaagaat tcaaggcttt gtgaatcctg cgccgcctgg
    13021 cgagcgatat tctgttgcaa atagttgtta gcgatgctgt tcagaatacg agtaatcaac
    13081 tggggatcat caccagtcat ggtaagttcc agcatcccgc tttctttact gcgttcgcta
    13141 acggtaaagg tttcctgcaa tgcgttaatc gcttccagtt cggtacgctg gctcaggaca
    13201 aactgtgttc ctggtttggc cttaatgtcc gcgatagtca gcgcaacgcc atctttttcc
    13261 agacgctggc cgaccatacc attgacggtg aactcttcac cttccagtgt atagtggccg
    13321 ttttccccaa ccgtgagtgt cagttgctga tcctgaccat tcagttgtgg aatatgcatc
    13381 cagctgatcg ccagctcacc tggtttttct ttggttaatc tcgcccagcc gcgacccaca
    13441 atcggaaaat acttctgctc aactatgtcg cgcagattca gttcagcaat ggttttaccg
    13501 agaatcatgc gcgattgcag cagttggatc tccggtgcag actcgggcga tgagttaggg
    13561 atcatatcgc tcaggccgct gagaatggcg ttgccctgtt tttgctcaac ctggaccaga
    13621 gtatctgcct gataaattgg tgtgcttaaa agcgagtaag cgacagcgat cagcgtgaat
    13681 aacgcggtca cgctgataat aaacttacgg tgatcccata actcgccgac cagacgaagc
    13741 agatcgatct cattttcctg agtgctgcct ggtggcgtat tcatattttt agttgtcatc
    13801 gtattttata aactcaaatt accggctgag gcgcttcgcc cattcctgac tggcgcgctc
    13861 caacataccg tagacatgtt caaatgcgtc ctgactttta cgatagggat ccgggatctc
    13921 tttctgttcc agccattgcc caaacagcat tgtcttcccg cggacctcgg gagcaattgc
    13981 ggtaacctga gcaatatgtt ccgactccat tgccagaatc agatcgtaat ttcgtgccat
    14041 ctcagcagtg agcttacgtc cggcatgtcc ttcaagagat acgccatgat tagcggcaac
    14101 atctgccgct gtcgcatctg cagggtgttt taccagacca tgaacacctg ccgatttgac
    14161 ttttacgccc ggcagacgtt tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt
    14221 gccggtacaa accaccagga ttgagttaaa ttttagttgg gccatgtttg aatccaccgt
    14281 gaagtctctg tcaggtcatg caccccggaa atcgtcggta ccagcagaga aatcacacgg
    14341 ttccaacgag ccagcggcgc ggtagtgaca taaacaatgt cgtaaggttc aagctggaat
    14401 tccgttccga gtatcattgc cgatgcgtcc ttcgcattca gctgataaat attggcgatt
    14461 ttgccattct gcttgttttg cgttgcacga ataacaaaaa tccccgtcgc atcagcgaca
    14521 tcctgattca taccctcagc gttacccagc gcttccgcca gggtcatacc gctgcggtcc
    14581 attttcagcg tgctctgttt acccacttca cccatcacga agactttcag atcatcgttg
    14641 cgcggaataa ataaaatatc gcccgggtgc agcagtttgt tctggcgcaa gtcgccacgc
    14701 tgcatcagcg catagaggtt cactttggtc tttacgccat tctgggtgag caccacgttg
    14761 cgccagtctg catctgcggt cagaccgccc gcggcgttaa tcgcatccat gatggtcaga
    14821 ggaatattgg taatcggctg ctgacccgat ttgctgactt cgccggtgac gtacgctttt
    14881 tgcgagcgga acgccgcgac gctgacatcg acttgcgggc tttcaatgac tgaatccaga
    14941 cgcgctgtaa tctcgttgcg tacctgggta agtgttttcc cggccacttt caggcgacca
    15001 atataggggt agaaaatcgc accatcggcg ttaacccagt tgccggtatc actggcgcta
    15061 cggtattgcc ctgcgggggt ggttagttcc gggtgatccc agaccgtaac catcaacaca
    15121 tccccaatgc cgatacgata ttcatagttt gcgatttgct gctccagctc cgggttagcc
    15181 tgcgacataa tcggtttggc gcgcaactga tcaatcaatc ctggcgtgac gggatagata
    15241 ttgaccattt tgtccaggtc atactgattg tcaggtagtt caatgacatc cttgttactg
    15301 gtcgataaat tctgaccagg aacaagggta catgctgtca aagacagaac agccaatgtc
    15361 agtaccgaaa acttaaaaat gttctttttc attggtttat atttatcagt caatgtaatg
    15421 ttatgaatta cgtcaggtta ttgtccaacc ggtacttctc gctctgccgt cattggatag
    15481 agctggtatt tgcgtgacag catttgtcca ccatcacgcg tcaatggtgt ccacgaaacc
    15541 accgcgcggt tgcggttagg tccaatggtc atcaagtcga atggaatcga gatataaaaa
    15601 cctttactaa agccgccttc gccgtagtca tctttagata cgttactgat tgccgcccat
    15661 acccctaccg ccacgccgct gtcaaagcgt ttggcgacgt cgatcgttgc gcctttatct
    15721 tttgccagat attgcccaac gctaagtttc atcagtacgc cattgagcgt cggcgggttc
    15781 cagtaagccg tcacgaaacc tgttggcgtg gaataatcgg tgaaacgcat catgttgtcc
    15841 cagtcacgtt gcttcacgta gttaacgtcc acacccagcg cccagctggc atctagcggg
    15901 cgataaagca gctcggaacc gacaccggcg tacatcgttt ccagataacc gccatacacc
    15961 tggccataga aaccattgcc taagtcggca aagtagttcg cctgcaagtt gttgagataa
    16021 acgtcattgc gaacgtaatc acggatatgc gtgcgcacgc ggggcagggt agagtccgcg
    16081 ggcaatagcg aagacttaaa cttgtcgtag ttgttgtaaa tattggtgaa aataccgcca
    16141 tcaagcagca ggtggtcggt aaaccagtag cgggcactgg acatcagccc cagctggaac
    16201 atatagaaat cttccgggcc gcccagcgac tgtgaaagtg ttgggttgaa agagtagcta
    16261 aagcgatctt cacgaatacg gtagccccga ccaaaggcag aaagatcttc tgcttcaaca
    16321 cgttgttgtt gaagttgctc tgattgacca ggcgctgtac cagccagctg tttgcgcagg
    16381 ctggctacgt cggtttcggt agtcaccatc gccatatgct cgcgcttttg cgtcacgcta
    16441 atcttctcaa cgccttgcgg caggttattc accagaatcc ggttggcacg atcgaccgct
    16501 tcgcgagaat cacggtattt gtattgttga ccagacatat acagcgtctt atcgcgcagc
    16561 tgaatttccg gtgcttcaaa acctgcgttg tacttcagcg cggtaagttg gttcgctact
    16621 gtggtgtact gcaatccttc agattcaggc gcaggttgat atgccggttt tggcgtatcg
    16681 cgcagggcag ggcgcagatc gttgaaattg gtccgtaacg tgaagccaaa catcaacgtg
    16741 ttaccgcgtt cataactcag gttgagatct gcccagctgg cagcgcgata aactgcgccg
    16801 acgttgaaat ggcttgcctg aggcagtttg ccagcgaaat cattctggta attgttgcca
    16861 tcgtattcga gtttcagacg caggggattc cacggcgttt gatactcaat gccgccaaag
    16921 atggaagccg ggccacgaaa gatatcgcta aagctgatat cgcccgcatc gtgagactct
    16981 gcgcgatgac aatatttatc gctcacccgg caaaacgggt tggtaatatt gcccgcatta
    17041 ccggcgtagc cccatgccat cccgagggtg aaatcaaatg gccccgcttg cttgctggcc
    17101 accagatact caccgtcaaa cagacccgta ccagcaatat cacgtttacc aaacgccact
    17161 tgcggtagcc agtaaccttc ttcccacagg cgaagtttaa aatcgaatga tttgtctttg
    17221 tatgactgat cgccgctgaa atcttcccac tggctatatt tgcgtgtgcg cacatccgta
    17281 taacgaatgg tgccttccag ccatgggaac agcgccacgg aggtggagta gaaccggtat
    17341 tgatcgttat cccggtagtt gacgctgaat tcaccttccg gtgcgatgcg cgcatttggc
    17401 atctgcaaca atcctgtgcc gccaaagtct gattgcgacg aacccagcgg atccgggtag
    17461 gttaatactt cagcatgaca ggcactggag acggcaatgg ccaggcagct taaaagataa
    17521 gaattcttct tcatcagtca ggaacacgtt gcgttaaaac agacacaatc tggtcattta
    17581 aatcagcata tttttcgggc agtacatgcg cagaaaagcc caaccaaagc tggctgccag
    17641 gcggtggttc cacatgtctt ttattccaca gggcaacagg ggcgacgaca gtctcaccct
    17701 caggcgtaat caccataacg ttattcttgt ccgcgcctgc aaggcggggg tgatcttgca
    17761 gataatcggt aacgctacgg cctgcctgcc agggcaattg cccggcccca gaaaccgcac
    17821 ccagcagagt aatagtcacc ggacgctgta cggtatagag cgtatagtcc ccgaccaacg
    17881 ggggattgct gttctcatcc actcgtacaa aatcagggtc cagtttgacc ggtaagcgac
    17941 cagtgatatt gagattgagt aactgctggc gcacggattt aatcgttgcc gccacatcgt
    18001 cgtcagcttc tgcttcccag gaagcaagct gtgccatgac atgttggtag tctttaagcg
    18061 ctttagcttt cgccgcagag tcggtcagca aggcaccagg ccaccagagg cgatcgcgca
    18121 gttgcggttg tgtcaccaac tggacaacat tttccatggg gccaacggaa agtgtctgtt
    18181 gttcgccagg cagataaata gtcaccgttc cttgcgcaaa ggcatggggt gtcattacgt
    18241 aaagtacgct ggcaatgaaa tacgactgta atttattcat ggtgcgggtt taagaaacgt
    18301 catttctacg ggaataaccc ctgcgcctaa catttgccga ctttgacgaa cctggccagt
    18361 agccgaatcg acccagaatg tgttttgcca ctgtttttct ggccgcgtgg aagtcacctc
    18421 ttcctgccag acattacaca gcacattgcg acccgcaata ttcagcgtct ctaatccagc
    18481 aaaggaaaaa gtagaactca gggtcgcaga gcgaaaatgg ttgtcttcac tccagagaat
    18541 atcgcgcgtc cagcggctgc catcttttat tgccagcgcg ttacgcagag ggtcctgccc
    18601 ggaatttgtc acttccagca gattattatt aagtttgacg gtttttaaaa gacgtccatt
    18661 gtgggtaacc agcatggcgt tatcctggga taaccatttg ctttgttctt gttctatata
    18721 tcccagtacc acaaagattc gttgcccttc attaaggcgt aaatacatcg tggaataagg
    18781 caacgcctgg atctgctgat ccgctacggt gatatcctga ttatcgaaaa ggctggcgcg
    18841 aaatgtatcg accatacttt gctggctgtg cgtacatccc gcaagaaata gtgcgaaaat
    18901 cgataaaata agagggcgca cctttaatcc ttgaaaggtt aattaaaaaa atgcaaatct
    18961 ctaaagcccg gtacgaatat cgcaaccggg tatcatttaa ctttaatttc gacgcgttaa
    19021 agcagtatta gcgtgtagtg gacgtggtgg ttgaggtgtt atgagaaccg ccatcgccac
    19081 tgtcatttgc tgcaacaact gccaacgtcg ccacaccaac agccgttgcc acaccgacaa
    19141 ctgtcgccgt gccgacagcg ccagcagcca cgccaccagt ggtagtagca gtcgtggttg
    19201 cgccggtagt ggtcgttgtg gtgccagtag tacccgtcgt tgcctcggtc ccgtttgttg
    19261 cttcaggggc agcaaacgca gctggcgtcg tcaacatgaa ggccataagg attgcagaaa
    19321 gcttgtgttt cataactttt cctttattca tcgcatggac aatacgtacg cttgagatca
    19381 ggattgtcta ctttgagtat aacatccttg tgaaatctcc ctttcctcat accgcttgca
    19441 ctatatactg cgggtaggaa aggcgcgcag agggaaatat aagattgtta actatccccc
    19501 tctggaaata cgacttgtga gcataagata aatcttagca aaatacaaat taaagataag
    19561 gaaattgtta cgaaagctat taattattgt tggtaatata gtttcaagtg gtactataac
    19621 tggctgttaa atggcattta taactattag gtgcagagat attcgcttaa atggattagt
    19681 ttctttgtgg aaaagtaact gatgttataa tgtatgacgt tgactattgc actattccaa
    19741 taaaaccagt cagctttaaa caagcagcgt catattaaga gagataaaca tttgccgctg
    19801 ttggtcctcg caggccattt acgcggcaaa attccacacg taatcctggt ataagcactt
    19861 ctgcgtcgcg gggagtgaat gcggaaatat ggacctgaac ttctttacga ccgtcggagg
    19921 ggataatgaa tcctttgccg cttttgcgat caaaggtttt gacaattcct gtcattttac
    19981 gggacaaaca aattccttac tgaaaatact gcgctgcact atacggggtt aataaaataa
    20041 agccagcgat atttaagacc gccggacggc taaaataaaa tttgcttaat ctcaattatc
    20101 atgcgttaat agctgcgtcg gtttgaaaga cagacagcat acaaagtagt ttactaaagc
    20161 agttctcatt atcaggcatt atccccttct tttgagtctc tctcctgaac actaagtagt
    20221 ttctgtatta aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa atatgtctaa
    20281 taaaatgact ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc
    20341 tgatgatggc agcaaagacg ttttcgtcca tttcactgcc atccagagca atgaattccg
    20401 cacgctgaac gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggccccgc
    20461 ggcagcgaac gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct
    20521 gtccatgttg tcatggttca cagtaccgta catcggcatt cgatgtgacg gagcgaaacc
    20581 ctttgggcgc taagtgtatt ttttgtaaat cgacgatgat cacctttgat aacgtcgcgc
    20641 tgcaaatacg cacggaccat gcgccgctgg atttcacaaa ataatatcag gctccctcgt
    20701 ggagcctttt ttatatctgc cttatttttc ttcaacgctg tatgtatagt aagcgataac
    20761 ctgttgatta ttgaatcttt cggggagatg gcttataaca tttcttacct gaccagggta
    20821 ccgggaacca acaccttact ggcgtgttgc tgtctttcaa gaccagaaga ggttaacagt
    20881 gaatattgaa gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa
    20941 aatcgccgag ctgaacaaga atacagggaa agaagtctct gaaattcgct tcaccgcacg
    21001 agaaaaaatg accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga
    21061 acatcacccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
    21121 ttgtggaatt aagcgtcggt acaattcatc cggtaccggg ctttgccata ctcccgcata
    21181 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
    21241 agacaacgca aaggcttttt tatttgccgc aggtttttgc agtgaaaagg tcaatgttga
    21301 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
    21361 aattaattta tcgaccggga tccgtgatgg gcaatttttt gcgcatttgc cacagtcgat
    21421 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
    21481 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
    21541 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
    21601 aacgtccatc accacgctgt agggcgacaa cataaaatag tgaatcgcct gagcgggcat
    21661 caataatgcg atatagagaa aaaaactcaa cagcaaatac ttcacgccgc gcagaggaat
    21721 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
    21781 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
    21841 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
    21901 acctgttgta aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
    21961 acctcccggt ctggcgacaa atgtcgtgct acttgccgtt tcgtaatagc gcacccaata
    22021 ccagaacgtg atggcaatat aaatattcat tgccaacagt aataattgcg tcgctttacg
    22081 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcctgtcg tgcctggccg
    22141 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
    22201 ccaaatgcca ctattctagt tgttcttaac tggctgatat tgattcaaat cgcgttcagg
    22261 tctttcttat tcaaccatgc ttccagatcg gcaacactgc gtgtaatttc ttcgtgtgga
    22321 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctggctggc gatacgcatt
    22381 cccaaactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctatcc
    22441 tgactggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tgtaaataat
    22501 accagccatt cgtggatctt ctccgtcccc attaactgag catcttcatt gagttgcgat
    22561 acatccagcg attgatcgtt attgacttgc agttggagat agtgcgccag taactgaccg
    22621 agcacttcac gcggcaccgg tttagggata atcccgcgga ataatgaact ggtacgctgg
    22681 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
    22741 tgtgccagtt gtcgggcaag cgtaatgccg tcgacatccg gcagatcaaa atccaccagt
    22801 gcggcagcaa acggttcgct attttgcagt gtctctaaag actgcgcggc attgccaaca
    22861 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
    22921 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt gactgttttt
    22981 gggacgggtg ccgtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
    23041 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
    23101 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
    23161 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
    23221 ccatcagtgc gactacgcag gataatgtac ccttcgtcag taaaacgcag ggcgttgctc
    23281 aacaggttgg ttataacctg acgaatacgt cgtggatcgc ccattaacgc gcacggcata
    23341 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
    23401 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac cgaaacattc
    23461 ttgccacctg cttcgatagc ggaataatcg agaatatcgt tgaggatggt cagcaacgat
    23521 tcaccagagt cagtaattgc ccgcaaatca tcacgctggg cgttaagtgc ggggttatct
    23581 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
    23641 gccgccagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
    23701 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
    23761 tcgcggtggc gattcagtgc atgaacattg ctgcgaaacg catccatcag ccgcccgatg
    23821 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
    23881 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg ataaaccacg
    23941 cgccagagga tcagaatcag tgcgcaaaga gaaactattc ccagcaataa caggctgtat
    24001 tgcccgcgtg cactcgcttt ttccagatgc gccagtccgt gctgattacg cagctcaatg
    24061 gtatcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat gttattttgg
    24121 gcgagagttt gcaggtgagt gctgatttca ctgtcctgct gatacagcgc cagtagatcg
    24181 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
    24241 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
    24301 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
    24361 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
    24421 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
    24481 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgcgccag gcgtgcgatc
    24541 tcatcggcgg cagcgactat ctgctgactg agttgccgtt gttgctggcg tagttgcaga
    24601 cgccgcccca ccagttcccc ttgctgacgt aatgaacggg agatctcctg ctcctgtttt
    24661 tcaatagcgg tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc attaatcttc
    24721 aggctttgtg cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt atcggcactg
    24781 gtcaggttct gcgcggcgaa aagttcccag gcgctggctt cgctcaactg gcgcgccata
    24841 ttcatggtag gaatcaatgc ctgagtgttg tctttttcca cctggctgat aaagcgcagg
    24901 ttgtaccatc ccaccagggt actggtcagg gttaacagcg ccatcagggc aaagcccatc
    24961 cagagtcttc gggtcagggt taaattcacg gtcggtgcac ttaaggtgaa aaaggttgag
    25021 tcgcaaagcg gaatgcatct agcataaagc cttattattg atgaggctat catgcgcgta
    25081 ctgctatttt tacttctttc ccttttcatg ttatcggcat tttcggctga taacctgttg
    25141 cgctggcatg atgcgcagca ttacacggtg caagcctcta cgccgcttaa agccaaacgc
    25201 gcatggaaac tgtgcgcgct ttatcccagc ctgaaagatt catactggtt atcgttgaac
    25261 tatggtatgc aggaggcagc tcgccgctat ggtgtggatt taaaagtgct ggaggcaggc
    25321 ggctacagcc agttggctac ccagcaagca caaatcgacc agtgtaaaca gtggggcgca
    25381 gaggccattt tgctcggtag tagcactacg tcatttcccg acctgcaaaa gcaggtagca
    25441 aatctgccgg tgatcgaact ggtaaatgct cttgatgatc ccctggtgaa aagccgcgtt
    25501 ggtgtaccct ggtttcagat gggctatcaa cctgggcgat atttggtgca atgggcgcac
    25561 ggtaaaccac tgaacgtgct gttgatgccc ggacccgata atgccggggg cagtaaagag
    25621 atggtcgagg gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt tgatattgcg
    25681 cttggtgata acgatattga aatccagcgt aacctgttgc aggagatgct ggaacgccac
    25741 ccagaaatcg acgtcgttgc cggaacggcc attgcggcag aggcggcaat gggtgaaggg
    25801 cgtaacctga aaacgccgct taccgtggtg tcgttttatc tttcacatca ggtgtatcgc
    25861 gggctgaagc ggggaagagt gattatggct gccagcgatc aaatggtctg gcagggggaa
    25921 ctggcggttg agcaggccat caggcaatta caggggcaat cggtgtctga taatgtcagc
    25981 ccaccgattt tagttctgac gccgaaaaat gccgaccgtg aacatattcg ccgctcgctg
    26041 tcaccagggg gatttcgtcc ggtctatttt tatcagcaca catcagcggc taagaaataa
    26101 ccttcaccat gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa acgacgaatt
    26161 aacacatcga cggtgcgcag gtcagggttt tccacccgac gcgcagaaag catacgtagc
    26221 agacgttcac ggctgagaat ttcgcccgga ttcgtcacaa atgccaccaa catttcatac
    26281 tctgcgcggg tcagtttaat cggctcgcca tcccgctcca gcgtatggcg cgacacattc
    26341 aggcaataac cggcaaagcg atagcagttg tcctgagtgt gcggttgagc ttgtcgcgcg
    26401 aggtcgattc gccagagcag atttttcacc cgtactacca gttcgcgcag ttccagcggt
    26461 ttggtgacgt aatcgtctgc gcccatttcc agcccaacaa tacggtcaat ccgatcgctg
    26521 cgtccggtaa ccagaataat ccccaccgtt gagcgttctc gcagggcgcg ggttaacatc
    26581 aggccatttt catcgggtaa gttgatatcc agcagaatta aatctaccgg ctgattctgc
    26641 ataatttccc gcagcccggc accgctcgct gtaacggaaa cggtataccc ctcctgagtg
    26701 aagtaagatt gtaatcgcgc ctgggtaacc ggctcatctt caacaataac aatgtgatgt
    26761 ggcatcagag ggttttactc attctgttca tatctgttca tattctgccg taagccgttc
    26821 atcctgacca gtgccgctgt tcatatttgc tcattaagat cgcttcacta aaccataatt
    26881 ttacaggggc tattatgcgg aaactctgga acgcgctacg ccgacccagt gctcgttggt
    26941 cggtactggc gctggtcgca attgggattg tgattggcat tgcgctgatt gtattgccac
    27001 acgttgggat caaagtcacc agcacaaccg aattttgtgt cagttgccac agtatgcaac
    27061 cggtgtatga agaatataaa cagtcggtgc atttccagaa cgcctccggc gtgcgagctg
    27121 aatgccatga ctgtcatatc ccgccggata ttccaggcat ggtgaagcgc aaactggaag
    27181 cgagcaatga tatctaccag acctttattg ctcattccat tgatacacct gaaaaattcg
    27241 aagccaaacg cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa aacaactcgg
    27301 caacctgccg ctcctgccat aactacgatg cgatggatca tgcgaagcag catcctgaag
    27361 cggcacgtca gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac tgtcataaag
    27421 gtattgccca ccagttaccg gatatgagta gcggcttccg taagcagttc gatgagctgc
    27481 gcgccagtgc taatgacagt ggtgacacgc tgtactctat tgatattaag ccaatttatg
    27541 cggcgaaagg cgataaagaa gcctctggtt ctctgctgcc tgcttcggaa gtgaaagtcc
    27601 ttaaacgtga cggcgactgg ctgcaaattg aaattaccgg ctggacggaa agcgccggac
    27661 gtcagcgtgt actcacccaa ttcccaggta aacgcatctt tgttgcctcg attcgtggtg
    27721 atgtgcagca gcaggtaaaa acgctggaga aaaccaccgt tgccgacacc aataccgagt
    27781 ggagcaagtt gcaggccact gcgtggatga agaaaggcga catggtgaac gatatcaaac
    27841 cgatctgggc ttatgcggat tcgttgtaca acggcacctg taaccagtgc cacggcgcac
    27901 cggaaatcgc ccactttgac gctaacggtt ggatcggcac gctcaacggc atgattggct
    27961 ttaccagtct cgataaacgt gaagaacgca ccttgttgaa atatctgcaa atgaatgcgt
    28021 ctgacaccgc aggtaaggct cacggcgata agaaggaaga aaaataatga acaataacga
    28081 tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg
    28141 gatgctgggg ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag cggcgactga
    28201 cgctgtcatc tcgaaagagg gcattcttac cgggtcgcac tggggggcta tccgcgcgac
    28261 ggtgaaggat ggtcgctttg tggcggcaaa accgttcgaa ctggataaat atccgtcgaa
    28321 aatgattgcc ggattgccgg atcacgtaca caacgcggcg cgtattcgtt atccgatggt
    28381 acgcgtggac tggctgcgta agcgccatct cagcgatacc tcccagcgcg gtgataaccg
    28441 ttttgtgcgc gtgagctggg atgaagccct cgacatgttc tatgaagaac tggaacgcgt
    28501 gcagaaaact cacgggccga gtgccttgct gaccgccagt ggttggcaat cgacggggat
    28561 gttccataac gcttcgggga tgctggcgaa agctattgcc ttgcatggta atagcgttgg
    28621 tacgggcgga gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg tggttggctc
    28681 aatggaagtg tatgaacagc aaacctcctg gccgctggta ttgcagaaca gcaaaaccat
    28741 tgtgctgtgg ggctctgatt tgctgaaaaa ccagcaagcg aactggtggt gcccggatca
    28801 cgatgtttat gaatattacg cgcagctaaa agcgaaagtc gccgccggtg aaattgaggt
    28861 catcagcatc gatccggttg tcacatccac ccatgagtat ctggggcgcg agcatgtgaa
    28921 gcacattgcg gttaacccgc aaactgatgt gccgctgcaa ctggcgctgg cgtatacgct
    28981 gtacagtgaa aacctgtacg acaaaaactt cctcgctaac tactgtgtgg gttttgagca
    29041 gttcctgccg tacctgctgg gtgagaaaga cggtcagccg aaagatgccg catgggctga
    29101 aaaactgacc ggcattgatg ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa
    29161 cagaacgcaa attattgctg gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc
    29221 gtggatgatt gtggttctgg cggcgatgct ggggcaaatt ggcctgccag gtggtggctt
    29281 tggttttggc tggcactata acggcgcagg cacgccgggg cgtaaaggcg ttattctgag
    29341 tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgatt acaaaggcta
    29401 cagcagcacc attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat
    29461 caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac
    29521 taacccattc catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga
    29581 aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct
    29641 gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg
    29701 tggcattatc gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga
    29761 tattttccgc gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga
    29821 cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg
    29881 cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca
    29941 tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg
    30001 cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga
    30061 ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc
    30121 gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagtt
    30181 atgtgagtcg gaaactctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat
    30241 taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa
    30301 cgctcgcggt caggtgttgg caggggcagt agtttctgac cgctatgcac ccggcgtggc
    30361 acgaattcac gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg
    30421 caaatacggt aaccccaacg tgctgaccat cgacatcggt acttcgcagc tggcgcaggc
    30481 gaccagtgcg cacactacgc tggtggaaat tgagaagtac aatggagcag tggagcaggt
    30541 gacggcgttt aacggccctg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca
    30601 ggtgaaatca tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg
    30661 cagttgttct cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg
    30721 gctgaatggt tttcgttgct gaaaagcgaa ccgccactgg ctgcggcggt gaatgagctg
    30781 gaaaactgta ttgccacgct gacagtacgt gacgatgccc gtctggaact ggccgctgac
    30841 ttttgcggcc tgtttctgat gaccgacaaa caagcggcgc tgccgtatgc atcggcctac
    30901 aaacaggacg agcaagagat taaacgcttg ttagttgagg cagggatgga aaccagcggc
    30961 aatttcaacg aaccggcaga tcatctggcg atctatctcg aattgctcag ccatctgcat
    31021 ttttcgctgg gagaggggac cgttcctgcg cgaagaatcg acagtttgcg gcaaaaaaca
    31081 ctgacggcgc tgtggcaatg gttaccagag tttgttgggc gttgtcgtca gtatgacagc
    31141 tttggttttt acgcggcact aagccagtta ttgctggtgt tagtggagag cgaccaccaa
    31201 aacagataac gtcgtttgtg cgcctgaaaa gacgcgttta gcgtcgcatc aggcattatg
    31261 gggcagttgc cggatgcgtc atcacatccg gcaaacacac atcacggatg agttacaaac
    31321 cgggaaagtc gctggcgcag caggcggttt tcctgcttca ggtgcgcaat atcatccatt
    31381 aacgtcagcg ccaccgcgat cccaggccag tccagagcca gttcatgacg caggcgtact
    31441 gcgcgttgca ccacgatggc ggcatggtcg tcaaataccc aggttgtttc ctggatctca
    31501 cgcggttcaa ccacccccaa accgacaatt tcattcaact cctcttcaga gatgccggta
    31561 tgcaggcaaa attcggtaat agtaaaagtc accgtaacat tagccattat gctttccccc
    31621 aatctttacg tggatcaaaa gacgactggg cgtctgccag ttgctgccac agcgcggcag
    31681 tgttttcatc cggtttcggc ggcatcacga ttttcagtac cgcatacaga tcgccggtct
    31741 gttttttgct caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc tggctgcctg
    31801 gcgggatagt cagcaaaatg ctttctttca gtgttggaac ggtgacttta gcacccagcg
    31861 ccgcttccca cgggctaacc ggcactacaa tttccagatc atggccgaca atatcgaaca
    31921 gcggatgtgg cgcaatatga atcaccagcc acaaatcgcc atttggaccg ccgttttcgc
    31981 ccggcgtccc ctggcctttc agacggatgc gttgaccatt gccgacgccc gccgggatct
    32041 tgacattcag cgttttcgga atttcctgtt cgatcatgcc aaaggcgtta taaaccggca
    32101 ggttatagct gatggtacgc ttatgctcag taagcgtttc ttcgaggaat accgccactt
    32161 cgatttcaat atcgtggccg cgtgtggcgg ggcgttgacg gctctggcgg gcatgctgac
    32221 cgaaaattga cgagaagata tcgtcaaaat cttcggcgtt aaaactctga ccgtcgctat
    32281 gatggaactg acggttaaat tgcggatcgt tgcgatgttg ccacatctga tcatactcag
    32341 cgcgacgttg ttcatcactt aacacttccc aggcttcagc gacctctttg aagcgggctt
    32401 cggcatccgg ttctttgctg acatcaggat ggtatttgcg ggccagtcga cgataggcgg
    32461 tcttgattgt cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa taatccttta
    32521 attccatagc gttatctcgc gtaaatcaac acaaattgaa ggaacccctg taagctaact
    32581 cctataagtg tagggtaatc ctcaaaattt catatgccaa cacagaatat gttattgaaa
    32641 tcatcgcgga gaggaggtcg cctcaaggtg gggtgctgaa catattttaa acaggtgaaa
    32701 aagggtgagc gatttttgat agtggaacca ggcactttaa gtttaactgg ggcgtcatta
    32761 tttattaaat tttataggcg ctatatatga gtagtaatat acatggaatt agttgcactg
    32821 caaataatta tttgaaacaa gcctggaaca atataaaaaa tgagtacgaa aaaaatcaaa
    32881 aatattcaat cacgcttttt gaaaacacac tggtgtgttt tatgcggttg tataaagaaa
    32941 tcagacgtca aaaagctgaa gactacattc catgcctgga atgcgattca ctagaaaaag
    33001 aatttgagga aatgcagaat gataatgatt tatcattatt tttgagaaca ttgcgtacta
    33061 atgatacaga aacttattca ggggtttcag aaggtattac atatactata caatatgttc
    33121 gagatattga tattgttaga gtgtccttgc cgggcagagg ttcagagtct atcacagatt
    33181 ttaaaggtta ttattggtat ggctttatgg aatatattga aaacattaat gcgtgtgatg
    33241 atgttttttc tgagtattgt ttagatgatg aaaatatgag tatccagcca gaatggataa
    33301 atacgccggg aatatctgat ttagatactg gtattgattt gtctggtata tcttttattc
    33361 agagtgaaat taataaaact tatggattaa aatacgctcc agtagatggc gatggctatt
    33421 gtctgttaag ggccatactg gttttaaaag aacatgagta ttcatgggcg ctgggcagcc
    33481 ataagacgca aaagcaagta tacgaagagt ttattaaaat tgttgataaa caaacgatcg
    33541 aggctcttgt tgatacggca ttcaatgatc tcagggaaga tgtaaagaca ttattcggcg
    33601 ttaatctaca atctgacaac aaaattcagg ggcagggtgg ttttttatca tggagttttt
    33661 tgtcttttaa aaaagaattt attgatagtt gcttgaatga caaaaaatgc atactgcatt
    33721 tacccgagtt tatatttaat gataataagg cacgccttgt tttagacacc gacccagagc
    33781 agaaggttaa tgaggtgaaa aatttcctca ctgctctttc agatagcatt tgctcattat
    33841 ttattgttaa tagtaatgtg gcatcaatct ccttagggaa tgaatccttt tcaacagatg
    33901 acgaccttga gtatgggtat ttaataaaca ctggcaatca ttatgacgtt tacctccctc
    33961 ctgaactttt tgctcaggct tacgagttaa acaataagga aaggaatgcg caaattgatt
    34021 ttttaactcg ttatgcaatt tactgacaaa ggcatgtgct aaaaaccatt gttattagcc
    34081 tcacttctct tattattggt actattgcct ctgtattagt aacgccgctg atattctgtt
    34141 tagccacagg tgtaatcatc agcggcgtac gcgaggcagg gagctaatca ggcatagttt
    34201 gcgtcaaacc ttgcctgttt ttaaagatgt atatagaaaa acaggcattc aacaagccat
    34261 tttgcgaacc tgttcccgga aaaatgtcat atttctgtca cactctttag tgattgataa
    34321 caaaagaggt gccaggaatg aacaaaacgc taatcgccgc aactgtggca gggatagttt
    34381 tactcgcttc aaatgctcag gcacaaaccg taccggaagg ctatcagcta cagcaagtgc
    34441 tcatgatgag ccgccataac ttacgtgcgc cgctggcgaa caatggcagt gtgctggagc
    34501 agtctacgcc gaataaatgg cccgaatggg acgtccccgg tgggcaactc accaccaaag
    34561 gtggcgtgct cgaagtgtat atgggccatt acatgcgtga atggctggca cagcagggga
    34621 tggtgaaatc gggggaatgc ccgccgccgg acaccgttta tgcctatgcc aatagtctgc
    34681 aacgtaccgt tgcgaccgca cagttcttta ttaccggcgc attcccgggg tgtgatattc
    34741 ctgtgcatca ccaggaaaaa atgggcacta tggacccaac ctttaacccg gtgatcaccg
    34801 atgattccgc cgcattcagt gaacaggcgg tggcggcaat ggagaaagag ctcagcaaac
    34861 tccagcttac cgacagctac caactactgg aaaaaatcgt taactataaa gattcccctg
    34921 cctgtaaaga gaaacaacag tgttcgctgg tggatggcaa aaataccttt agcgccaagt
    34981 atcaacaaga accaggtgtt tccgggccgc tgaaagtcgg caactcgctg gtagatgcgt
    35041 ttactttgca atattacgaa ggttttccga tggatcaggt ggcctgggga gaaatcaaat
    35101 ctgaccagca gtggaaggtg ttgtcgaagc ttaaaaacgg ctaccaggac agcctgttta
    35161 cctcaccgga agtggcgcgc aatgttgcga aaccgctggt cagttatatc gacaaagctc
    35221 tggtcaccga tcgcaccagc gcaccgaaaa ttacagtgtt ggttgggcac gactccaaca
    35281 ttgcctctct gttaacggcg ctggatttca aaccgtatca gttgcatgac cagaacgaac
    35341 gcacgccgat tggcggcaaa atcgttttcc agcgttggca tgacagcaaa gccaatcgcg
    35401 atttgatgaa aattgaatat gtgtatcaga gtgcggaaca gttacgtaat gccgatgcgt
    35461 taaccctgca ggcacctgct cagcgtgtga cgctggaatt aagcggttgc ccgatagatg
    35521 ccaacggttt ctgcccaatg gataagtttg atagcgtgtt gaatgaggcg gtgaaataac
    35581 agaaaactcc cccgcgagaa gtgggggagt cgctggttaa acgtttttac gttcgatggt
    35641 ctgttcgccc caaaaaagcg aatctttatc ggtcttagca aaggctttga ctaacacttc
    35701 atcgctacct tcttcccaaa tcttttccgc cattttttcg tcgtacccgg cgacttcgaa
    35761 aatggcctcg gctatttccg gcgacgtatt gcgcagagat gcccattcac cgacgtgatg
    35821 agctttcgct tcttgagttg gcatgcgtat cctcctgttg aagattagcc gttaagttta
    35881 actgccagac ctgcgacata ttccccttga taacgagcaa tagacagttc ttcctggctt
    35941 ggctggcgtg agccgtcacc gcctgcgatg gtggttgcgc cgtacggcgt accgccgcga
    36001 acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa tggggacaat taccatgccg
    36061 tgatgcgcaa gggtcgtcca ggttgaagta atagtttgtt cctgaccgcc gccagtaccg
    36121 gtggaactaa agacgctcgc cagttttccg tatagtgcgc cggaagccca caggccgccc
    36181 gtctggtcga ggaaggtacg catttgaccg gacatgttgc caaagcgggt aggtgtacca
    36241 aaaataatgg cgtcgtaatc ggccagttct tgcggggttg caaccggtgc agtttgcgtt
    36301 ttaccgcctg ctttttcaaa taattgcggc ggcatggttt ccggtacacg cttaacgaca
    36361 acttctgcgc catcgacttt gcttgcaccc tcagcgactg cgcgtgccat cgtttcaata
    36421 tgtccgtaca tggaataata aagcaccaga actttagcca tttctaacca ctcctcgtgt
    36481 tatctctatt ccgtagcgat tcgctaccac ttatttaaag ataagacgtc cttttcagag
    36541 tgcaaatttc acaaccactt atttgattta taacaacttt cacaagcacg taattttgtc
    36601 gcaaaatgac acattttatc tcatcgcgtt tttttaatca taagagcggc ttatggataa
    36661 ttatttgaga tgatatttat tctcgctaag aagctgttgc aggatattac caaacgcggg
    36721 tctgcccgcg tcagttcact aagcttagtc ccacgtagcg aaaatatggc agccgccata
    36781 cgccgcgtta attctatgca atatgatgtc tatacccaga cggaggtcag taatggcaaa
    36841 ccatcgaggc ggttccggca attttgcaga agaccgcgaa agagcatcag aagcaggtaa
    36901 aaaaggtgga cagcacagcg ggggtaattt caagaatgac ccgcagcgcg catctgaagc
    36961 aggtaaaaaa ggtggcaaga gcagtcacgg caaaagcgac aactagccgg gctaatcaat
    37021 gacgaatgca tttttttctt aggctcgtca aaaagtcatc accgccggta atcccggcgg
    37081 ttttctgttt atggctcctg atgaacaact tctggcggtg gaacgtcaac caactttctg
    37141 cttaacaacg cattgagtaa aatcgcgcca aaggttgctg taccaatccc tcccaacgta
    37201 aaaccgccca gcgtgagagc aaaatcaccc gcgcccagca ctaaggttac tgcgaccata
    37261 atcaaattac cgttctggct taaatcgaca cggttttgta cccatatcct tgcgcctgcg
    37321 acggcaatca gcccgaacac aacaattgat gcaccaccaa tcaccgcggc cggaatggta
    37381 tgaatcagcg cgccaaattt cggtgaaaag cccaacagca tggcgatgac ggcagcagcg
    37441 acaaacacca gcgtcgagta gacttttgtc acggccatta caccgatatt ctcggcgtag
    37501 gtggttacgc cgctgccacc gacagaacca gaaagcatcg tcgccagccc atcaccgacg
    37561 aatgcccgcc ccatatacgg gtccatattg cgtccggtca tcccggcgac cgccttgaga
    37621 tgacctaagt tttccgccac cagaatcacc gccacgggcg caatcagcat cattgcctga
    37681 ctattaaaag caggagtgga aaaatgtggc aaaccgaacc aggcagcatg gctgacgaga
    37741 gtaaaatcga cggcttttcc cagccctaaa aggttggtca tcacgccata cagcagacag
    37801 gcgacaatta aacctacgag aatcaataac cgctggatca tgccacgggt aaacaccgcc
    37861 accagcccaa tacacagcac cgtcattacc gccatccagc tatcaaaggc cgaagccgat
    37921 acacttttca ctgcgatagg cgctaagttc aggccaatcg ccatcaccac cgcacccgtc
    37981 accaccggcg gcattagtcg ttcaatccag cgtgtaccaa ttttcatcac caccaggcca
    38041 atgacggtat aaaccagccc acaggcgata atcccgccca gcgcaatgct gatattcggg
    38101 ttaatgccct gaccgttaaa gcccgtcgcg gcaatcacca cgccgacaaa agccgcgctg
    38161 gaaccgagat aactggggac gcgcccgccg gtaataaaga aaaacagtaa cgtgccgatc
    38221 cccgacatta aaatggaaag attgggatcc agccccatca gaatcggcat taacaccgtc
    38281 gcgccaaaca tcgccaccgc gtgttgaacg cccattactg ccgtctgagc aaacggcaat
    38341 cgttcatacg gcgcgaccac gccgctctct gtagaggtcg attttaactg ccagtgagga
    38401 aaaccgaaca ttgccatcag ctgtctcctt taggaggtta acaagcaggg cgcatcagcg
    38461 cgtgataact gcgatcgaac cacaccagcc cgtagggtgt ggcgtgacga tgaatcgctt
    38521 cgatggcgca aaacagaatg tcgtgggtgc cgacgctcac cacctggctg atacggcagt
    38581 caaacgaaac cagagcctct tccagttgcg ggcatccggt cacacccgtc tgccagcggg
    38641 cggcggcaaa gcggtgttcc atgggcgttt tgccgccaaa aagatttgaa agcggctcct
    38701 gcccggcgct cagtgtattt acacacagcg ttcgattttc attgaatacc ggccagacgg
    38761 acgccccacg attcaggcac accagtaatg taggcggcgt gtcggtcacg ctgcagaccg
    38821 cgctggcggt gaacccggcg cgcccggctg gaccatccgt ggtgatgata ttgactgccg
    38881 cgcccataca ggacatcgca tcgcgaaagg tttgttgatc gacaatgttc atggtttgct
    38941 ccttacaaca gcccgcaggc ttcttcaaag gacagacgtg gcaggcgcgc aaaaagtttg
    39001 ctgctatcgc catagccgat attaatcagc agattgctct tcagcgtgct gcccgcaaaa
    39061 aaggcgtcgt caacgtgttg acggtcaaaa cccgacatcg ggccggtatc cagtcccagc
    39121 gcacggcagg cgacgatcag ataggccgcc tgcatggaac tgttgcgaaa cgctgtttct
    39181 tcggcaagtt gtgggctgga ggtaaaccaa ctgcgggcat cgccgtgagg aaacagttgc
    39241 ggtaaccgtt cataaaattc actgtcccag gcgacgatag cggtgacggg cgcggtcagg
    39301 gttttttgca gattgccgct ggaaagtgcc gggcgcagac gttcttttcc ttctgccgtg
    39361 cggataaaca cgatccgtgc cggagaacag ttagctgatg tcggccccca tttcatcagg
    39421 gcataaatct cccgtaacgt ctcatcgctg acgggtgtct cccgccagcc gttgtgagtg
    39481 cgggcatcgg tgaacaaggt gctaagcgca cctgggctaa cggcttcgtt catagcaatt
    39541 ccttacaggg cggcttcacg gtgatgtaac aggctggcaa gcccgttgag taacagagca
    39601 ttaaacgttt cgggatcggt cacgttgcag gcgtgtccgc catagggcat caccattttc
    39661 tggctatcgg gcagggcggc atgaagctca ctggaacatg ctgatggcac cagcagatca
    39721 tcactggcgc agatgatttg caccgggcag cggatgcgat ccgcatggtg actaaagtca
    39781 gcgcgtttga gggcgttaag tcgacgcagt aaattatttt tgccctgaaa atgcgccagt
    39841 gccagcgcgt cttctgcctc caggcgaggt gcgcgggccg ccatccagtc ggcgggatag
    39901 aggaacaacg gctgcgcttc cacccatgcc tgcgcgccgc cgctatacag taatcgttcg
    39961 cgaacctgaa aacagcggcg cgtatgggcg tttattcgta gccagccgtt aacgctgatc
    40021 agcacagtta ccgacgcggg ataatccagc gccagctgca ttcccaccag cgcaccgagc
    40081 gcatggccga ccaccgcgta atgctcaatc cctgcggcta ccagcgcctg atgcagttcc
    40141 gctgccatct gggcgatact gtaatcttct gccagcgtgt cgggattatt gccggtgccg
    40201 cgctggtcgt aacagactac ctgatactcc tgctccagca ccgccagttg cggtaaccag
    40261 taactgccgc taccgccaag acccgaaatc aacaccacta cgggcgcatc agcataaggg
    40321 ggaggtgaga gtgaaagttt catcgcggcc tcacttggcg atatgcgcaa ttgtggcgat
    40381 ttccaccagc gcgtcaggtt ttaccagtcc gcactgaatg cagaatcgcg ccggtttatc
    40441 acccggaaaa aactcggcgt agatttcgtt aatcgcggcg taatttttcc agtcggtaat
    40501 aaagatgctg ttgaaggtca catccgccat cgtgccaccc gccgtctcga tcaccttgcg
    40561 gataatttcc agaacgtggc gggtttgcgc ctttgggtca tcggcaaaca gcacgttatt
    40621 atgttgatca aaagccagcg taccggagac atacaccacg ccatcagcca gcgtgccggg
    40681 aacgaagggg gccagcggtg cgctgctgcc agcgggaata attacggatt ttggcatcgt
    40741 taaactcctt aagcgatacg agcaaaggat gtgggagaaa gcgcgtcgca gaacgtttcg
    40801 acgtcgctga cccagccaaa aaaggtttcg atattgaaca acgcggcttt ctgtgcaaat
    40861 tccggccccg cctggtgtgt tgcgtcttcc agcaccacgc cgaaatactc cagaaaaaag
    40921 ccatcgcgta gcgtcgattc gacgcagacg ttggtagcga taccggtgaa aaccagatgg
    40981 cgtattccac ggctgcgcaa aatgctgtcc agcggtgtat tgaagaaacc gctgtagcgc
    41041 ggcttcggca gcacaatatc gccaggctgc ggcaccagtt catccaccag ttgataatcc
    41101 caggagcctt tcgccagcaa tttcccctgc agctgcggct gcttacgcat ggttttcagg
    41161 gcgttcgatt tatgaaaatt cggtgagccg gggccgccag cttcgacata ctgttcatcc
    41221 cagccatttt gaaaccagat gatcagcatc cctgccgctc gcgctgcggt cacggcggtt
    41281 tgaatgttgg caatgaccgg gcgagtggtt gagacatcaa acccggcgag atctaagtag
    41341 ccgcctggcg tggcataagc gttttgcata tccaccacga tcagcgcact ttgctgcgga
    41401 tcgaaggtaa tggcttccgg tcgagcggtt aaggttgtca tcatgccacc tcctgagtca
    41461 gcgcaggtag atgggcgcgg cactgcatca gtggttgaat gcgctcgccg aaggtttcga
    41521 ttcccgacag aaaatcatcg aaggtcaaca gcacgccttc ggcaccaggc acgcttgcga
    41581 cttcatctaa catgcgcgcg acactggcgt aagaaccgac taacgtcccc atattgatgt
    41641 ttaccgccga agtgggatcg gccatctgac ggacgttggt atcagtaccg gagcgggtat
    41701 ctttctgact ttgttcggtt agccagctta acgcctcttc atccgcgccc gctttgtagt
    41761 gttcccattt ggcgcgagcg gcatcgtcgg tttcatcggc aatcaccata aacaacacat
    41821 aagaaccaac gtcgcgcccg gtttgctctg cggcctgttt catccgcgca gcggtcgggg
    41881 cgaaagccgt gggcgtattt acgcctttgc cgaaacagaa gttgaaatcg gcatactggg
    41941 cggagaacgc catgccagcg tcgctttgcc cggcgcagat cactttcatg gggacactcg
    42001 gttgcggact gacgcgacaa tcattcatgg tgaaaaaatc gcctttaaaa tcgcttttcc
    42061 ccgtgcccca caggtcgcgc agcacctgaa catattcggt gagatagtcg taacgacggg
    42121 agaaatagtc atcgccaggc cagatcccca tctgctcata ctcgggcttt tgccagccag
    42181 tcacgaggtt gacgccaaaa cgcccgccag agatggagtc gatggttgcg gccatacggg
    42241 cgacgattgc cggaggtaac gttaaggtgg cagcagtggc gtaaatctga atgcgcgagg
    42301 tgacggccgc cagccccgcc atcaaggtga acgactcaag gttgtgatcc cagaactcag
    42361 ttttgccgcc aaagccacgc agtttgatca tcgacagggc gaaatcgaaa tggtagtgct
    42421 ccgctttttg cacaatggct ttattcagtt caaaggtcgg catgtactgc ggcgcgtggg
    42481 tcgaaatgag ccagccgttg ttgccaatag gtacgaatac gccaattttc atcatcaacc
    42541 tctcttcgtc tcgtaaagtg aaagtcagac ggggcgctgc atcctgcata tccttttcag
    42601 ccgcgtaatg gcttgtttgc aaagcggatg ccagttttta aaaagtgaat gttattaatg
    42661 tgttaacatt gtgttgtcta aaatatctgg taaaaagtgg actaaacggt caaaacagtt
    42721 gcacataaaa catgcatctg tgcgcgatga gagtgcagaa ggtcgaggcc gggcgggggt
    42781 tttgctatcc tgttgccaat ctacaagagg ggagagcgca tgacgcaagg cgcagtgaaa
    42841 acaacgggta aacgttcgcg cgcagtaagc gcgaagaaaa aagcgattct tagcgcagca
    42901 ctggacactt tttcacaatt cggttttcac ggcacaaggc tggagcagat cgcagagttg
    42961 gcgggtgttt caaaaaccaa tctgctgtat tactttccgt caaaagaggc gctgtatatt
    43021 gccgtgctgc ggcagattct cgatatctgg ctggcaccgt taaaagcgtt tcgtgaagat
    43081 ttcgctccgc tggcggcgat aaaagagtac atccgtctga agctggaagt ctcccgcgat
    43141 tatccgcagg cttcgcgcct gttctgtatg gagatgctgg caggcgcgcc gctgttaatg
    43201 gatgaactga cgggcgattt gaaggcatta attgatgaga aatcggcgct gattgccggt
    43261 tgggtcaaaa gcggcaaact cgcgccgatt gatccgcagc atttgatttt catgatttgg
    43321 gcttccactc aacattacgc cgatttcgcc cctcaggtgg aggcggtgac aggcgcgacg
    43381 ttgcgcgatg agatattttt caatcaaacg gttgaaaacg tgcagcggat tattattgag
    43441 gggattcgac cacgttaaag atgccggagg aggttgtaac atcctccggc tacctgttta
    43501 acctatagtc attaaactgg cgttaccgcc agcggcagcg gtattcacac tcagcgaacg
    43561 ttcgatatac agccgttcca gaaggatatt gctttcgcca cgggcaaaac cctgcaccga
    43621 aacaattgcg ccatcccgcg cagcaacggc ttcacacaat gcgcgaagct gatccgaatc
    43681 accgtggaag atcaccgcat caaagggttg agcggttata ttttccgctt tcgccagttg
    43741 aatacgttcg ctgactgccg atggcaatgc ctttactagc tgacgatgca gcgcgtcatc
    43801 cggccacagt acctggctgc ccaccgccag cacggcggcg agctgagtca gcgcatcctg
    43861 ctcatcatcg gcaatacaca acacgcgctc acgcggcagc agcgtccagg tgttgcgttc
    43921 acccgtcggc cccggcagca atcgttgtgt tcctgcctgc gccagctcgc catattgcgt
    43981 acataacgcc tgcaattctg gacgatttgc tgcccattcc cgcagtgcat ttagcggctg
    44041 agtcaatgcg gctttcaact gtgcatcgac cggatactct gcatcctgac gcgcgagcgt
    44101 cactgccagc gcactttccg ggcgattcgc cagcagacgg tagagataga gcggaccgcc
    44161 tgctttcggc ccggtaccgg acaacccttc gccgccgaat ggctgcacac ccaccactgc
    44221 gcccaccata ttacggttaa catacaggtt accaacatgg gccgagccag tgacctgggc
    44281 gatggtttca tcaatacgcg tatggacacc aagcgtcaga ccatagccgg aagcgttaat
    44341 ctgctcgatc agctctggta gctggttacg gttgtaacgc accacatgca gcaccggacc
    44401 aaagacctct ttttgcaatt cggcaaagtc atccagttcg atcagcgtcg gggcgacaaa
    44461 ggtgccgctt tgccattcac gggcatcttc gctgttttcc cgcaccgcct ggaacaccgg
    44521 acggcctttg ctacgcatgg tctgaatatg gcgctcaata ttggctttcg cttcgctatc
    44581 aatcaccgga ccgatatcgg tggtcaggcg acccggatta cccatccggc attcggccat
    44641 tgcgccgcgc agcattttca acgtgtggtc ggcaatctca tcttgcaggc acagcacgcg
    44701 cagcgccgaa caacgctgac ccgcactgtc gaacgccgag gccagtacat ccacgacgac
    44761 ctgttcggtc agtgctgaag aatcgacaat catcgcgttc atgccgccgg tttcagcgat
    44821 gagcggaata gggcgaccct gagcgtccag gcggctggcg atattgcgct gcagtaacgt
    44881 agcgacttcg gttgaaccgg taaacatcac cccgcgtacg cgatcatcac ccgtcagttg
    44941 cgcgcccacg gtttccccct gacctggcag caactgcacc acgcctggcg gtacacccgc
    45001 ttccagcaaa atggcgatcc cttgcgcggc aatcagcggc gtttgttctg ccggttttgc
    45061 cagcacgctg ttacctgccg ccagtgcggc agcaatctgc ccggtgaaaa tagccagcgg
    45121 gaagttccac ggactgatac acaccacagg ccctaatgga cggtgggttt cgttagcgaa
    45181 atcatcccgc acctgtccgg catagtagtg gagaaaatcg accgcttcgc gcacttcggc
    45241 aatggcgtta ctgaaggttt ttccggcttc acgcaccaga ataccaatca gttgctgcat
    45301 ctggctttcc attagcacgg cagcgcgatg caaaatcgcc gcgcgttcaa ccggaggagt
    45361 ggcaaaccag attggcgcgt tattaaccgc actttccagc gcctgttcta cctcacgcgg
    45421 cgtggcttca cgcacaaagc ccacaatatc tttcggttcc gcagggttaa taacgggcga
    45481 catctcacct gccgctaccg gttgttccag cattggcaag gcctgccatt tttgcagtgc
    45541 actattgagc agggcagaag agagcgaggc cagccggtgt tcgttagcga gatccagccc
    45601 tgccgagttg tcgcgcccgt gaccgtaaag atcgcgcggc aggggaattt tcggatgcgg
    45661 taatccagtt tgcccttcct gttgcgccag tttttctaca gcagtgaccg gatcggcgac
    45721 cagttcatcc agtggcaaag aggtgtcggc aatacggtta acaaacgagg tgttagcacc
    45781 gttttccagc aggcgacgca ccagatacgc caacagcgtt tcatgggtac cgaccggagc
    45841 ataaatacga cacggacggt taagtttgcc gtcggcaact ttcccggtga cctgctcata
    45901 cagtggctcg cccataccat gcaggcactg gaactcgtac tgacccggat agtagttctg
    45961 acctgccagt tgataaatcg ctgccagcgt atgggcgttg tgcgtcgcga actgcgggta
    46021 gattagattc ggcaccgcca gcagcttttt cgcacaggcg agatatgaaa cgtcggtata
    46081 caccttgcgg gtataaaccg gatagccttc aaggccgtcc atctgcgcac gcttgatttc
    46141 actatcccag tacgcgcctt tcaccaggcg aatcatcaga cggcgacggc tgcgggtggc
    46201 gagatcaatc aggtaatcaa tcaccagtgg gcagcgtttt tgataagcct gaataacaaa
    46261 accgataccg ttccagcctg ccagctcagg ctcgaaacag agtttttcca gcagatcgag
    46321 ggagatctcc agacgatcgg cctcttcggc gtcaatgttg ataccaatat cgtactgacg
    46381 cgccagcagg gtgagtgatt tcagacgtgg gtaaagctct tccattaccc ggtcatattg
    46441 ggcgcggctg taacgcggat gcagcgccga cagcttgatt gaaatgcccg gaccttcata
    46501 gatgccacga ccattggacg ctttaccaat ggcgtgaatc gcctgctgat aggaaaccat
    46561 atacgcttgt gcatctgcgg cagtcagtgc agcttcgcca agcatatcgt aagagtaacg
    46621 gaaacctttc tcttccagct tgcgggcatt ggctaacgct tccgcgatgg tttcgccagt
    46681 gacgaactgc tcgcccatca ggcgcatcgc catatccaca cctttgcgga tcagcggttc
    46741 accgctttta ccgataatgc ggttcagcga gcgggagagg ctggcttcgt tatgggtgga
    46801 aaccagtttg ccagtaaaca gcagccccca ggtggcggca ttaacaaaca aagacgggct
    46861 acgaccaatg tgtgattgcc agttaccgtt gctgattttg tcgcgaatta acgcatcacg
    46921 ggtggctttg tcgggaatac gcaacaacgc ttccgccaga cacatcagcg ccacgccttc
    46981 ctgcgatgac aacgaaaact cctgcaataa cccctgaacc atacctgcgc gaccactggc
    47041 gtttttttga ttacgcagtt tatcggccag ttgatacgcc agtttgtgcg cctgttcagc
    47101 aactggctgc ggcaggcggg cttgttccag cagcatagaa accgcttcgg tttccgggcg
    47161 gcgataggcc gctgtgatcg cggcgcggga aaccgactgg ggcaaaattt gctcggcaaa
    47221 gtcgaggaat ggttggtgtg gttcctctgc cggagtcggt gcttcatcgc tctcattggc
    47281 agcgccagaa agcagcgcag gtagctccgg tagagtatcg ctgttttcca gttgttcgag
    47341 ataagaaaaa atcgcctgct taattaacca gtgtggcgtg cgatcgatac gtgtcgcggc
    47401 agacttaata cgctcacgcg tcgcgtcgtc cagcttaacc cccatggtgg tggttcccat
    47461 gccattactc ctgttgttct gaaaggtgca acttaacgtt atcgtgaaat atccatgatg
    47521 ttgcaacttt gtgcaaccat gttaaatgtg acatgcgtag caagcttaaa aatgaatgaa
    47581 atgttaataa aagaaatcga tatgacaggg attaaaaaaa taactcagac tttttctctg
    47641 cggcagttaa catttttgaa aggtgcaacc gcaaaaaatg tgagagagtg caacctgatg
    47701 aaaaatagtg tcgctgagca ctaaaattta atgtaaatgg tgtgttaaat cgattgtgaa
    47761 taaccagcgc ttccggcagg atacggtcgc cctggtaaaa cataaactct gttaccccgt
    47821 tccggtggca gatataacgg caagtttcga cattgccgat aataattttt tggagacttt
    47881 agatggctat tagcacaccg atgttggtga cattttgtgt ctatatcttt ggcatgatat
    47941 tgattgggtt tatcgcctgg cgatcaacga aaaactttga cgactatatt ctgggcggtc
    48001 gtagccttgg gccattcgtg acggcattat cggcgggtgc gtcggatatg agcggctggc
    48061 tgttaatggg gttgccgggc gcggtttttc tttccgggat ttccgaaagt tggatcgcca
    48121 ttggcctgac attaggcgcg tggatcaact ggaagctggt ggcggggcgg ttgcgtgtgc
    48181 ataccgaata caacaataac gccttaacat tgccggatta tttcaccggg cgctttgaag
    48241 ataaaagccg cattttgcgc attatctccg cgctggtgat tttgctgttc ttcaccattt
    48301 attgcgcttc gggcattgtg gcaggcgcgc gtctgtttga aagtaccttt ggcatgagct
    48361 acgaaacggc tctgtgggcg ggagctgcgg cgacgatcct ttacaccttt attggcggtt
    48421 tcctcgcggt gagctggact gacactgtac aggccagcct gatgattttt gccctgatcc
    48481 tgacgccggt tatcgtcatt atcagtgtcg gtggctttgg tgactcgctg gaagtgatca
    48541 aacaaaagag catcgaaaac gttgatatgc tcaaagggct gaactttgtc gccattatct
    48601 cactgatggg ttgggggctg ggttacttcg ggcagccgca catcctggcg cgttttatgg
    48661 cggcagactc tcaccacagc attgtccatg cgcgtcgtat cagtatgacc tggatgatcc
    48721 tctgcctggc aggggcggtg gctgttggct tctttgggat tgcttacttt aatgagcacc
    48781 cggcggtagc aggcgcggta aaccagaacg ccgagcgcgt gtttatcgaa ctggcgcaaa
    48841 ttctgtttaa cccgtggatt gccgggattc tgctgtcggc gattctggcg gcggtaatgt
    48901 caaccttaag ttgccagctg ctggtatgtt ccagtgcaat taccgaagat ttatacaaag
    48961 cgtttctgcg taaacaggcc agccagaaag agctggtgtg ggtaggacgt gtgatggtgc
    49021 tggtggtggc gctggtggcg attgcgctgg cggcaaatcc ggaaaaccgc gtgctgggct
    49081 tagtgagcta cgcgtgggca ggctttggcg cggcgtttgg tccggtggtg ctgttctcgg
    49141 tgatgtggtc acgcatgacg cgcaacggtg cgctggcggg gatgatcatc ggtgcgctga
    49201 cggttatcgt ctggaagcag ttcggctggc tgggactcta cgaaattatt ccgggcttca
    49261 tcttcggcag tatcgggatt gtagtgttta gtttgctggg taaagcgccg tcagcggcga
    49321 tgcaaaaacg ctttgccgag gccgatgcgc actatcattc ggctccgccg tcacggttgc
    49381 aggaaagcta agggacttag cctgcggcgg ttttgtttgg cttcagcagc gggttgcgct
    49441 cccttaatgt gcctcgccat ataaattgaa tggtgcaggg agcgcgcagg gggcggccaa
    49501 tcgccgcggc cccctgcacc cccgggctct ggcgaacaaa atcgccgctg cgcggtacct
    49561 tcagcttatc cccttcggct accgggtcgg gcgcgaggta acgtcctgta agacgcgccc
    49621 tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgtttcagcg attttgacgc
    49681 caccaacacc cgcgctgttc ttatgttccg ggcaaaaagt tagatttgat aatcgaggat
    49741 ggacgaaatt gcttgataca cccgcttatc agttttacat ggaagttttg atgtattgag
    49801 tcaggacggt tttgtcggct ggatacggcg tttacgcggc atccggcaag aacacatggc
    49861 tcgttgtaaa caatcccatc tttgtacctg gaataatcgt ttatatcccc tggcattacc
    49921 tctctttgtt tacatttcaa catcatttta taaacattcc gcttgtattt ttctttaccg
    49981 taatgataat cgctatcact gcgatttact tttctttgca tagattgact cagaaaaacg
    50041 tttaagggtg ggtggcatgt ttgttccgtt tctcattatg ttgcgcgaag gacttgaagc
    50101 cgcgctgatt gtcagtttga ttgccagcta tcttaagcgt acccagcgag gccgatggat
    50161 tggtgtgatg tggattggcg tgttgcttgc cgctgcgttg tgcctgggct tggggatctt
    50221 cattaacgaa accaccggcg aatttccgca aaaagagcag gaactgtttg aaggtatcgt
    50281 ggcggtgatc gccgtggtga tccttacctg gatggttttc tggatgcgca aagtgtcgcg
    50341 caacgtcaaa gtacaactgg agcaggcagt cgatagcgca ttgcagcgtg gaaatcatca
    50401 tggctgggcg ctggtgatga tggtcttttt tgccgttgca agggaagggc tggagtcggt
    50461 ctttttcctg ctggcggcat ttcaacaaga tgtcgggatc tggccgccgc tgggtgcaat
    50521 gctcggtctt gctactgccg tggtgctagg cttcttgctc tactggggcg gtattcgcct
    50581 caatcttggt gcatttttta aatggaccag cctgtttatt ctcttcgtcg ccgcagggct
    50641 ggcagctggt gccattcgcg catttcatga agccggattg tggaaccact ttcaggaaat
    50701 cgccttcgat atgagtgcgg tgctctcaac tcactcgctg tttggcacgc tgatggaagg
    50761 gatttttggc tatcaggaag cgccgagcgt cagcgaagtc gccgtctggt ttatttatct
    50821 catcccggcg ctggtggcat ttgctctgcc accacgcgca ggggcgacag cgtctcgctc
    50881 cgcgtaacaa atacgacgca aactcttgct tagttacaac atactttaaa gggatagtct
    50941 cgtcatgacc attaacttcc gccgtaacgc attgcagttg agcgtggctg cgctgttttc
    51001 ttctgctttt atggctaacg ccgctgatgt gacgcaggtc aaagtgaccg tgacggataa
    51061 gcagtgcgaa ccgatgacca ttacggttaa cgccgggaaa acacagttta ttattcagaa
    51121 ccatagccag aaggcgctgg agtgggagat cctcaaaggc gtgatggtgg tggaagagcg
    51181 ggaaaatatc gcccctggtt ttagccagaa aatgacggcg aatttacagc ctggcgaata
    51241 cgatatgacc tgcggtctgc tgactaaccc gaaagggaag ttgatcgtca aaggtgaggc
    51301 aacggcggat gcggcgcaaa gtgatgcgct gttaagtctt ggtggtgcaa ttactgcata
    51361 taaagcgtat gtcatggcgg aaaccacgca gctggtgacc gacaccaaag cctttaccga
    51421 cgcgattaaa gcaggcgata tcgaaaaagc gaaagcactg tatgcgccga cgcgccagca
    51481 ctatgagcgc attgaaccga ttgctgaact gttctccgat ctggatggca gcattgacgc
    51541 ccgtgaagat gattacgagc aaaaagccgc tgatccaaaa ttcaccggtt tccaccgtct
    51601 ggaaaaagca ttgtttggcg acaacaccac caaaggcatg gatcagtatg ctgaccagct
    51661 ttataccgat gtggtcgatt tgcaaaaacg catcagtgaa ctggctttcc caccttcaaa
    51721 agtggtcggc ggtgcagccg gactgattga ggaagtggca gccagcaaaa tcagcggtga
    51781 agaagatcgc tacagccaca ccgatctgtg ggatttccag gctaacgttg aaggctcgca
    51841 gaaaattgtc gatctgctgc gtccacaact gcaaaaagcc aacccggaac tgttggcaaa
    51901 agtcgatgcc aactttaaaa aggtcgatac cattctggcg aaataccgta ctaaagacgg
    51961 ttttgaaacc tacgacaaat tgaccgatgc cgaccggaat gcactgaaag gaccgattac
    52021 tgcgctggcg gaagatctgg cgcaacttcg cggtgtgctg ggattggatt aagcattatg
    52081 cagtatgaag ataaaaacgg cgtgaatgaa ccgtcacgcc gacgtttact gaaaggcatt
    52141 ggtgcgctgg cgctggcggg aagttgtccg gtcgctcatg cacaaaaaac gcaaagtgcg
    52201 ccgggtacgc tttcaccgga tgcacgcaat gagaaacagc cgttttatgg tgagcatcag
    52261 gcagggatcc tgacgccaca acaggccgca atgatgctgg tggcgtttga tgtgcttgcc
    52321 agcgataaag ccgatcttga acggttgttt cgcttgttga ctcagcgttt tgcttttctg
    52381 actcagggcg gagcggcacc agaaacgcca aatccgcgcc tgccaccact cgattccggc
    52441 attcttggcg gctacattgc gcccgataat ctcaccatca cgttatcggt gggtcactca
    52501 ttgtttgatg agcgctttgg ccttgcgcca cagatgccga aaaagttgca gaagatgacg
    52561 cgttttccca acgactcgct ggatgcggcg ttatgtcatg gtgatgtgtt gctacaaatt
    52621 tgcgccaaca cccaggacac ggtgatccat gcactgcgcg atatcatcaa acatacgccg
    52681 gatttgctca gcgtgcgctg gaagcgggaa gggtttattt ccgatcacgc ggcgcgtagt
    52741 aaaggcaaag agacgccgat taatttgctg ggtttcaaag acggcactgc caatcccgat
    52801 agccaggatg agaagttgat gcaaaaagtg gtgtgggtaa cggcagatca gcaggagcca
    52861 gcgtggacaa tcggtggcag ctatcaggca gtacgcttga ttcagtttcg agtggaattt
    52921 tgggacagaa ctccgctgaa agaacagcag acgatttttg gtcgtgataa gcaaaccggt
    52981 gcgccgctgg gaatgcagca tgagcatgat gttcccgatt acgccagcga tccggaaggg
    53041 aaggtgattg cgctggacag ccatatccgg ctggcgaatc cccgcacggc ggagagtgag
    53101 tccagcctga tgctgcgtcg tggctacagt tattcactgg gcgtcaccaa ctccgggcaa
    53161 ctggatatgg ggctactgtt tgtctgttac caacacgatc tggaaaaagg cttcctgaca
    53221 gtacaaaaaa ggctcaatgg cgaagcgctg gaggaatacg ttaaacctat cggcggcggt
    53281 tatttttttg cgctgccggg agtgaaggac gcgaacgatt atctcggaag cgcgttattg
    53341 cgggtttaat gtttatagac ggataaggcg ttcttgcgca gatgcctgat gcgatgcttg
    53401 cgcgtcttct tatgcctaca atcggtgcgg gttcggtagg cggataaggc gttcacgccg
    53461 catccggcgt tcgtgcgcag atgcctgatg cgaatcctgc tgaaagcaca cagctttttt
    53521 catatctgtc atcactctgt catctttcca gtagaaacta atgtcactga aatggtgttt
    53581 tatggttaaa tataagtgaa tatattgttg caatgaatgc gagatctgtt gtaattatta
    53641 agtagcagcg gaagttcccg gcagtgatag tcagtcacta tggagatcgc ggatggtaac
    53701 gtcctgttct ggacaatgtc ttgttaagct acgctccacc acttgcggag atttctcctc
    53761 cggtagcaac ttcgtcactc tgtatttttc tcacttcctc tctttactcg tgttaccgac
    53821 gccgcaaacg gtgtgcgtag ccgtttttcg gcattttatc caaaagcaaa ctatggctta
    53881 caaggaagcc aaccctctga tgttcgtgcg cataatcgcg ctgccaacgg cgcgtgtgat
    53941 gaatacaaac aactcaaggt gctctccatg ggaagacaaa aagcagtgat caaagctcgt
    54001 cgcgaggcaa aacgtgtgct gagacgggat tcgcgcagcc ataagcagcg tgaagaagaa
    54061 tcggtcacct cgcttgtgca gatgggcggc gtagaagcca ttggtatggc ccgcgacagt
    54121 cgcgatactt cgcccatcct cgcgcgaaat gaagcgcaat tgcactatct gaaggctatt
    54181 gagagtaagc agctgatatt cgccacgggc gaagctgggt gcggaaaaac ctggatcagc
    54241 gcagcaaaag cggcagaagc cctgatacat aaggatgtcg acaggattat cgtcacccgt
    54301 ccagttctgc aagccgatga agatcttggc ttcttacctg gagatatcgc agaaaagttt
    54361 gctccctatt ttcgcccggt ctacgacgtg ctggtccggc gcttaggggc ttcctttatg
    54421 cagtactgcc tgcgaccgga aatagggaag gtggaaattg cgccgttcgc ctatatgcgt
    54481 ggacgtacct ttgaaaatgc agtcgtcatt cttgacgagg cgcagaatgt gactgccgcg
    54541 caaatgaaaa tgtttttaac ccgcctcggg gagaacgtga cggttatcgt caacggtgat
    54601 atcacgcaat gcgatttgcc tcgcggcgtg tgctccggat taagtgacgc gctggaacgt
    54661 ttcgaagaag atgaaatggt tgggatcgtc cggttcggta aagaggactg cgtacgttcg
    54721 gcactttgcc aacgtacgct gcatgcctac agttaagtgt attatcggtg cagagcccgg
    54781 gcgaaccggg ctttgtttta tttcaacaga ttttcacttc aggttagcgt tcaggggggc
    54841 gactttgagc gtgttgataa gctgaactgg agtctggttt agatactctt accaggcatt
    54901 tttcgtcata gcatcagacc agtgtaatac tgccagcgcg gagagttttt cttccagaag
    54961 ccaaaaaaat gagcatctgc ctatcttttt tcggccattc aacctgtaca ggagatctgg
    55021 tgccaatttt gcttatgagg tttaatcaac aatgggctat actttgactg actgtttaac
    55081 accgaatata ttcctctgat ttattcttta gggataatat tataaatgta aggttaatct
    55141 taattaattc tttgctaata ttgtgtgttg ttattttttt gtggaaggca agccgcaatg
    55201 gctaaatagg gatggttgta ataatcaaaa catgatacta actatatatt gatcgttgca
    55261 ttgttggcat gtaacacgct gcagcgagta acattggtaa tgaaatataa tcaacatgga
    55321 taggtgaaaa ttaattatga gctttatggc ttttgtaact tgtttgacaa ttgcatttat
    55381 cgccggtttt gctgttgcca gaaaattgac actccgaaaa gttgcctcat cggtaatcat
    55441 cgctaaatcg caacccgtcc cttcatctgc aacttcccct gccgctaagg cgtcgacaag
    55501 gagctatata ccgcagaaaa cctatcgtga ctctcgttgg caagagcgag atgagttatt
    55561 tcagggaatg gttgccgaag gtattttatt ggaaagaaaa gagtggttaa tcacgcgttg
    55621 tgaaaaaatg tatttcgata atctggaaaa atggtttgga cattattgtc atatccattg
    55681 ccaggtctct ctggggcgct tgatcaactt tccagaacaa cctgagttta gcgaagaaga
    55741 aagaaagcgt ttctttgcaa tttataatgg catggcaatg gattatgttc tggtttcaaa
    55801 gaagaacaat aaaatagtct gtgttattga gctggatgac ccgagtcatg aaagggcgga
    55861 tcgcattatt cgtgaccgtt ggttggatac aattatgact cttgcacggg tgccttttgt
    55921 acgagcttct gtcactaaat tggaggaaat tcctccggtc tgggagactc gtgacaaaat
    55981 tcgcccatta gtcgcggcga caaagagtga accggtgcat taactaagca tcccggtcgg
    56041 ggcttctcat ccccccggtg tgtgcaatat acgaaaaaaa gcccgtacct tcgtacgagc
    56101 tcttctttaa agatggcggt gaggggggat tgactcgctt cgctcgccct gcgggcagcc
    56161 cgctcactgc gttcacggtc tgtccaactg gctgtcgcca gttgtcgaac cccggtcggg
    56221 gcttctcatc cccccggtgt gtgaaatata cgaaaaaaaa gcccgtactt tcgtacgagc
    56281 tcttctttaa atatggcggt gagggggacc gcctgaaata aatctgactt aatccagggg
    56341 tgttatataa tttcattata aacaatgtat tacacttttt gttgtccgat attatctaat
    56401 gtattcttag tgaagcgaga gactaatggc atactggatg gcataccaga gtgacgggaa
    56461 ttagcgtatg gcagtattga cggatacgaa agcaagacat atcaaacctg atgacaaacc
    56521 attgccccat gggggaatta ccggactgac ccttcatcct tcttcagtaa aggggcgggg
    56581 gaaatgggtt tttcgttatg taagtccggt gacacaaaaa agacgtaatg ctggattggg
    56641 aacttaccca gaggtcagta ttgctgaagc tgcacgtact gcccggataa tgcgagagca
    56701 acttgctgca ggtgatgatc ctctggagat taaaaaggct gaatctgaga aagtcgctat
    56761 cccaacattt gccgatgcag ccaggcgtgt acatgcagaa ctgtctcctg gatgggaaaa
    56821 tccaaagcat gtaaggcagt ggttatcgac gcttgagaat tacgcgtttc ctcaactggg
    56881 agcaaaaacg ctggattcga ttacggctgc ggacgtggca gaaacactgc gtccagtctg
    56941 gttaaccttg tcagaaacgg caagccgggt taaacagcgc attcatgttg ttatgcagtg
    57001 gggatgggcg cacggttttt gtgtagcaaa tcctgttgat gtggttgacc atttgcttcc
    57061 tcagcagaca agaggacgtg atgaacacca acccgcaatg ccctggaggc agttaccgct
    57121 ttttgtggcg accagtgtgt ataccgatga accttataat gttacccgcg cactgttatt
    57181 aatggtgata cttacagcaa ctcgctcggg cgaagccagg ggaatgcgct gggctgaaat
    57241 tgattttcat aagcgggtat ggactatacc tgcagaaaga atgaaagcca ggatacagca
    57301 tcgtgttcct ttatcccggc aggctattta cattctggaa aatatacgtg gcctgcatga
    57361 tgaactggtg ttcccttcac ccagaaagca gcagatcctt tccgatatgg tgttgacaag
    57421 ttttctgcgt aaaaagaaag ccgtcagtga cattccgggg cgagttgcca cggcacatgg
    57481 ttttcgctca acattcaggg actggtgtag cgaacagggg tattcgcggg atctggcgga
    57541 aagggcgctc gctcatacgc tgaaaaataa ggttgaggcg gcatatcatc gtactgatct
    57601 actggagcag cgtgtaccga tgatgcaggc atgggcggat tatgtgatgt ctcaaattgt
    57661 gaataaataa tagtgaaaat gtatattgta cgatcatgat ttgatatgtg atcgtagcag
    57721 tagtaattaa gaaaccgctt ttatgtcaaa tgtattgagc tgataattat cagatcgtca
    57781 ggaaagcgta ttttgattaa caggggatta cttaacaatc ccctttttat aaaaaataat
    57841 ttgcctgttt aactcttcac tcctgctgag actgaattat tcctagtgcg tcataaatca
    57901 gtcttctgcg tagtttattg tt
//
LOCUS       DAZUFB010000018        57886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZUFB010000018 DAZUFB010000000
VERSION     DAZUFB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.018"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..1299
                     /gene="citC"
                     /locus_tag="ACHY3M_001300"
     CDS             241..1299
                     /gene="citC"
                     /locus_tag="ACHY3M_001300"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFS4316243.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1314..1610
                     /gene="citD"
                     /locus_tag="ACHY3M_001301"
     CDS             1314..1610
                     /gene="citD"
                     /locus_tag="ACHY3M_001301"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFS4316244.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1607..2515
                     /gene="citE"
                     /locus_tag="ACHY3M_001302"
     CDS             1607..2515
                     /gene="citE"
                     /locus_tag="ACHY3M_001302"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFS4316245.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2526..4058
                     /gene="citF"
                     /locus_tag="ACHY3M_001303"
     CDS             2526..4058
                     /gene="citF"
                     /locus_tag="ACHY3M_001303"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFS4316246.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLSYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTQVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4062..4613
                     /gene="citX"
                     /locus_tag="ACHY3M_001304"
     CDS             4062..4613
                     /gene="citX"
                     /locus_tag="ACHY3M_001304"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFS4316247.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4588..5466
                     /gene="citG"
                     /locus_tag="ACHY3M_001305"
     CDS             4588..5466
                     /gene="citG"
                     /locus_tag="ACHY3M_001305"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFS4316248.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5517..6980
                     /gene="citT"
                     /locus_tag="ACHY3M_001306"
     CDS             5517..6980
                     /gene="citT"
                     /locus_tag="ACHY3M_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFS4316249.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPRAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7094..7900
                     /gene="rna"
                     /locus_tag="ACHY3M_001307"
     CDS             7094..7900
                     /gene="rna"
                     /locus_tag="ACHY3M_001307"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFS4316250.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8130..8540
                     /gene="rnk"
                     /locus_tag="ACHY3M_001308"
     CDS             8130..8540
                     /gene="rnk"
                     /locus_tag="ACHY3M_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFS4316251.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8678..8767
                     /gene="yldA"
                     /locus_tag="ACHY3M_001309"
     CDS             8678..8767
                     /gene="yldA"
                     /locus_tag="ACHY3M_001309"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFS4316252.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8771..10009)
                     /gene="ybdR"
                     /locus_tag="ACHY3M_001310"
     CDS             complement(8771..10009)
                     /gene="ybdR"
                     /locus_tag="ACHY3M_001310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFS4316253.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10230..10658
                     /gene="uspG"
                     /locus_tag="ACHY3M_001311"
     CDS             10230..10658
                     /gene="uspG"
                     /locus_tag="ACHY3M_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFS4316254.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10779..12344)
                     /gene="ahpF"
                     /locus_tag="ACHY3M_001312"
     CDS             complement(10779..12344)
                     /gene="ahpF"
                     /locus_tag="ACHY3M_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4316255.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12515..13078)
                     /gene="ahpC"
                     /locus_tag="ACHY3M_001313"
     CDS             complement(12515..13078)
                     /gene="ahpC"
                     /locus_tag="ACHY3M_001313"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4316256.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13450..14196
                     /gene="dsbG"
                     /locus_tag="ACHY3M_001314"
     CDS             13450..14196
                     /gene="dsbG"
                     /locus_tag="ACHY3M_001314"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFS4316257.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14405..15307
                     /gene="ybdO"
                     /locus_tag="ACHY3M_001315"
     CDS             14405..15307
                     /gene="ybdO"
                     /locus_tag="ACHY3M_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4316258.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENVEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNFKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15454..16674
                     /gene="ybdN"
                     /locus_tag="ACHY3M_001316"
     CDS             15454..16674
                     /gene="ybdN"
                     /locus_tag="ACHY3M_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS4316259.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16647..17275
                     /gene="ybdM"
                     /locus_tag="ACHY3M_001317"
                     /pseudo
     CDS             16647..17275
                     /gene="ybdM"
                     /locus_tag="ACHY3M_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(17276..18436)
                     /gene="ybdL"
                     /locus_tag="ACHY3M_001318"
     CDS             complement(17276..18436)
                     /gene="ybdL"
                     /locus_tag="ACHY3M_001318"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFS4316260.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18545..19633
                     /gene="hcxA"
                     /locus_tag="ACHY3M_001319"
     CDS             18545..19633
                     /gene="hcxA"
                     /locus_tag="ACHY3M_001319"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFS4316261.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19643..19840)
                     /gene="ybdD"
                     /locus_tag="ACHY3M_001320"
     CDS             complement(19643..19840)
                     /gene="ybdD"
                     /locus_tag="ACHY3M_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS4316262.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(20022..22127)
                     /gene="cstA"
                     /locus_tag="ACHY3M_001321"
     CDS             complement(20022..22127)
                     /gene="cstA"
                     /locus_tag="ACHY3M_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFS4316263.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQTCFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22308..22721)
                     /gene="entH"
                     /locus_tag="ACHY3M_001322"
     CDS             complement(22308..22721)
                     /gene="entH"
                     /locus_tag="ACHY3M_001322"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFS4316264.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGMELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22724..23470)
                     /gene="entA"
                     /locus_tag="ACHY3M_001323"
     CDS             complement(22724..23470)
                     /gene="entA"
                     /locus_tag="ACHY3M_001323"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFS4316265.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23470..24327)
                     /gene="entB"
                     /locus_tag="ACHY3M_001324"
     CDS             complement(23470..24327)
                     /gene="entB"
                     /locus_tag="ACHY3M_001324"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFS4316266.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24341..25951)
                     /gene="entE"
                     /locus_tag="ACHY3M_001325"
     CDS             complement(24341..25951)
                     /gene="entE"
                     /locus_tag="ACHY3M_001325"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFS4316267.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25961..27136)
                     /gene="entC"
                     /locus_tag="ACHY3M_001326"
     CDS             complement(25961..27136)
                     /gene="entC"
                     /locus_tag="ACHY3M_001326"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFS4316268.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27505..28461
                     /gene="fepB"
                     /locus_tag="ACHY3M_001327"
     CDS             27505..28461
                     /gene="fepB"
                     /locus_tag="ACHY3M_001327"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4316269.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(28465..29715)
                     /gene="entS"
                     /locus_tag="ACHY3M_001328"
     CDS             complement(28465..29715)
                     /gene="entS"
                     /locus_tag="ACHY3M_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFS4316270.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29826..30830
                     /gene="fepD"
                     /locus_tag="ACHY3M_001329"
     CDS             29826..30830
                     /gene="fepD"
                     /locus_tag="ACHY3M_001329"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFS4316271.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30827..31819
                     /gene="fepG"
                     /locus_tag="ACHY3M_001330"
     CDS             30827..31819
                     /gene="fepG"
                     /locus_tag="ACHY3M_001330"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFS4316272.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSITMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31816..32631
                     /gene="fepC"
                     /locus_tag="ACHY3M_001331"
     CDS             31816..32631
                     /gene="fepC"
                     /locus_tag="ACHY3M_001331"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS4316273.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32628..33761)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY3M_001332"
     CDS             complement(32628..33761)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY3M_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFS4316274.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(33977..37858)
                     /gene="entF"
                     /locus_tag="ACHY3M_001333"
     CDS             complement(33977..37858)
                     /gene="entF"
                     /locus_tag="ACHY3M_001333"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFS4316275.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(37855..38073)
                     /gene="ybdZ"
                     /locus_tag="ACHY3M_001334"
     CDS             complement(37855..38073)
                     /gene="ybdZ"
                     /locus_tag="ACHY3M_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFS4316276.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(38076..39278)
                     /gene="fes"
                     /locus_tag="ACHY3M_001335"
     CDS             complement(38076..39278)
                     /gene="fes"
                     /locus_tag="ACHY3M_001335"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFS4316277.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            39521..41761
                     /gene="fepA"
                     /locus_tag="ACHY3M_001336"
     CDS             39521..41761
                     /gene="fepA"
                     /locus_tag="ACHY3M_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFS4316278.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            41801..41899
                     /locus_tag="ACHY3M_001337"
                     /pseudo
     CDS             41801..41899
                     /locus_tag="ACHY3M_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41936..42556
                     /gene="entD"
                     /locus_tag="ACHY3M_001338"
     CDS             41936..42556
                     /gene="entD"
                     /locus_tag="ACHY3M_001338"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFS4316279.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCKQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(42678..42830)
                     /gene="hokE"
                     /locus_tag="ACHY3M_001339"
     CDS             complement(42678..42830)
                     /gene="hokE"
                     /locus_tag="ACHY3M_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFS4316280.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(43181..43333)
                     /gene="hokE"
                     /locus_tag="ACHY3M_001340"
     CDS             complement(43181..43333)
                     /gene="hokE"
                     /locus_tag="ACHY3M_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFS4316281.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            43302..43571
                     /locus_tag="ACHY3M_001341"
     CDS             43302..43571
                     /locus_tag="ACHY3M_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316282.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            43775..44893
                     /gene="ybdK"
                     /locus_tag="ACHY3M_001342"
     CDS             43775..44893
                     /gene="ybdK"
                     /locus_tag="ACHY3M_001342"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFS4316283.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            44959..45207
                     /gene="ybdJ"
                     /locus_tag="ACHY3M_001343"
     CDS             44959..45207
                     /gene="ybdJ"
                     /locus_tag="ACHY3M_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFS4316284.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            45272..45640
                     /gene="ybdF"
                     /locus_tag="ACHY3M_001344"
     CDS             45272..45640
                     /gene="ybdF"
                     /locus_tag="ACHY3M_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4316285.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            45734..46387
                     /gene="nfsB"
                     /locus_tag="ACHY3M_001345"
     CDS             45734..46387
                     /gene="nfsB"
                     /locus_tag="ACHY3M_001345"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFS4316286.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            46495..47742
                     /gene="ybdG"
                     /locus_tag="ACHY3M_001346"
     CDS             46495..47742
                     /gene="ybdG"
                     /locus_tag="ACHY3M_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFS4316287.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(47810..49186)
                     /gene="pheP"
                     /locus_tag="ACHY3M_001347"
     CDS             complement(47810..49186)
                     /gene="pheP"
                     /locus_tag="ACHY3M_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFS4316288.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(49288..52431)
                     /gene="cusA"
                     /locus_tag="ACHY3M_001348"
     CDS             complement(49288..52431)
                     /gene="cusA"
                     /locus_tag="ACHY3M_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFS4316289.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLLLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(52443..53666)
                     /gene="cusB"
                     /locus_tag="ACHY3M_001349"
     CDS             complement(52443..53666)
                     /gene="cusB"
                     /locus_tag="ACHY3M_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFS4316290.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(53682..54014)
                     /gene="cusF"
                     /locus_tag="ACHY3M_001350"
     CDS             complement(53682..54014)
                     /gene="cusF"
                     /locus_tag="ACHY3M_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFS4316291.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(54172..55545)
                     /gene="cusC"
                     /locus_tag="ACHY3M_001351"
     CDS             complement(54172..55545)
                     /gene="cusC"
                     /locus_tag="ACHY3M_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFS4316292.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            55702..56385
                     /gene="cusR"
                     /locus_tag="ACHY3M_001352"
     CDS             55702..56385
                     /gene="cusR"
                     /locus_tag="ACHY3M_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFS4316293.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYQVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            56375..57823
                     /gene="cusS"
                     /locus_tag="ACHY3M_001353"
     CDS             56375..57823
                     /gene="cusS"
                     /locus_tag="ACHY3M_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFS4316294.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        13927 a        15774 c        14647 g        13538 t
ORIGIN      
        1 gtaattacat taattaaaaa acttaaaacc attaaataaa taaaaaacca ctaactctat
       61 gtgaaataaa tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa
      121 aagatatacc tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca
      181 acattgaaca acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt
      241 atgttcggca atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa
      301 atcgcccaat tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt
      361 accgtaaccc gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa
      421 tgcgttgcta tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa
      481 ctgataaacc tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa
      541 tacgaggcgc tgttccgcca gtgcggtttt tccacgctga ccagcgtacc cggcgtgatg
      601 gtgctgatgg aaaacagcgc cacgcggctg aaacgctatg ccgaatcgct gaaaaaattt
      661 cgtcatccag ggaacaagat tggctgcatt gtgatgaacg ccaatccctt cacgaatggt
      721 caccgttatc tgattcaaca agctgcggca cagtgcgact ggttgcatct gtttttagtc
      781 aaagaagatt cttcacgctt cccctatgaa gaccggttgg atctggtgtt aaaaggcacc
      841 gccgatattc cacgcctgac tgtgcatcgc ggctccgaat acatcatctc ccgcgctacg
      901 ttcccttgct acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat
      961 ctgaagattt tccgtcagta cctcgctccc gcgctgggtg taactcaccg ctttgtcggt
     1021 actgaaccct tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa
     1081 acgccgacta tctccgcacc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag
     1141 gagatgccga tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct
     1201 atcgcgccgc tggtccctgc agtcacgctg cattatttgc agaacctgct tgagcactcc
     1261 cgccaggacg cggcagctcg tcaaaagacc cccgcatgag aaacaggtga aaaatgaaaa
     1321 taaaccagcc cgccgttgca ggcacccttg agtctgggga tgtgatgata cgcatcgccc
     1381 cactcgatac gcaggatatc gacctgcaaa tcaatagcag cgttgagaaa cagtttggcg
     1441 atgcaattcg caccaccatt ctggacgttc tcgcccgcta caacgtgcgc ggcgtacagc
     1501 tgaatgtcga tgacaaaggc gcactggact gcattttacg tgcacgactg gaagccctgc
     1561 tggcacgcgc cagcggtatc ccggctctgc catgggagga ttgccaatga tttccgcttc
     1621 gctgcaacaa cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg gtgccaatgc
     1681 cgcgatggtc agcaactcct tcatctaccc ggctgatgcc ctgatgtttg acctcgaaga
     1741 ctccgtggca ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg cgctgcaaca
     1801 tccgctgtat cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt ccgaatgggg
     1861 tgttaacgac ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc tgccgaaaac
     1921 cgataccgct caggatgttc tggatattga aaaagagatc ctgcgtatcg aaaaagcctg
     1981 tggtcgtgaa cccggcagca ccggcctgct ggcggcgatt gaatctccgc tgggcattac
     2041 ccgcgcagtg gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc tcggtgcaga
     2101 agactatgtg cgcaacctgc gtacagaacg ctccccggaa ggaactgaac tgctgttcgc
     2161 acgctgttcc attttgcagg ccgcgcgctc tgcgggtatt caggcgttcg ataccgtcta
     2221 ttccgacgct aacaacgaag ccggattcct gcaagaagcc gcccacatca aacagctggg
     2281 ctttgacggc aaatcgctga tcaacccgcg tcagattgat ctgttgcaca acctctacgc
     2341 accaacccag aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg aagccgccgc
     2401 tcgcgaaggc ctcggcgtgg tttccctgaa cggcaagatg gtggacggtc cggttatcga
     2461 tcgcgcccgt ctggtgctct ctcgtgcaga actttccggc atccgcgaag aataaggcaa
     2521 tcaaaatgac gcagaaaatt gaacaatctc aacgacaaga acgggtagcg gcctggaatc
     2581 gtcgcgctga atgcgatctt gccgctttcc agaactcgcc aaagcaaacc taccaggctg
     2641 aaaaagcgcg cgatcgcaaa ctgtgcgcca acctggaaga agcgattcgt cgctctggtt
     2701 tacaggacgg catgacggtt tccttccatc acgctttccg tggcggtgac ctgaccgtca
     2761 atatggtgat ggacgtcatc gcgaagatgg gctttaaaaa cctgaccctg gcgtccagct
     2821 ccctgagtga ttgccatgcg ccgttggtag aacacattcg ccagggcgtg gttacccgca
     2881 tttatacctc cggcctgcgc ggtccactgg cggaagagat ctcccgtggt ctgctggcag
     2941 aaccggtgca aatccactct cacggcggtc gcgtgcatct ggtacagagc ggcgaactga
     3001 atatcgacgt ggctttcctc ggcgtcccgt cctgcgatga attcggtaat gccaacggct
     3061 acaccggtaa agcctgctgc ggctccctcg gctatgcaat ggttgatgcc gacaacgcaa
     3121 agcaggtcgt gatgcttacc gaagaactgc tgtcttatcc gcataatccg gcaagcattg
     3181 agcaagatca ggttgatttg atcgtcaaag ttgaccgcgt tggcgatgct gcaaaaatcg
     3241 gcgctggcgc gacccgtatg accactaacc cgcgcgaact gcttattgcc cgtagcgctg
     3301 cggatgtgat tgtcaactca ggctacttca aagaaggttt ctccatgcaa accggcaccg
     3361 gcggcgcatc gctggcggta acccgtttcc tggaagacaa aatgcgtagc cgcgatattc
     3421 gcgccgactt cgcccttggc ggtattaccg cgacgatggt tgacctgcac gaaaaaggtc
     3481 tgatccgcaa actgctggat gtgcagagct ttgacagcca tgctgcgcaa tcgctggccc
     3541 gtaaccccaa tcacatcgaa atcagcgcca accagtacgc taactggggt tcgaaaggcg
     3601 catcggttga tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac acccagttca
     3661 acgttaacgt gctgaccggc tctgacggcg tactgcgtgg tgcttccggt ggtcactgcg
     3721 ataccgcgat tgcctctgcg ctttccatca tcgtcgcgcc gctggtacgc ggtcgtattc
     3781 cgactcaggt ggataacgtg ctgacctgca tcaccccggg ctccagtgtc gatattctgg
     3841 tcacagacca cggtatcgca gttaacccgg cacgtccgga actggcagaa cgtctgcagg
     3901 aagcgggcat taaagtggtt tccattgagt ggctgcgcga acgtgcgcgt ctgctgaccg
     3961 gtgaaccaca gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt taccgcgatg
     4021 gctcggtgat cgatgttgtg catcaggtga aggaataagc catgcacctg cttcctgaac
     4081 tcgccagcca ccatgcggtg tcaattcccg agctgctcgt cagccgggat gaaaggcaag
     4141 cacggcaaca cgtctggctc aagcgccatc ctgttccact ggtctccttt accgtggttg
     4201 cgcctgggcc gattaaagac agcgaggtca cacgccgaat ttttaatcat ggcgtgacag
     4261 ccttgcgtgc cttagccgca aaacagggct ggcaaattca ggagcaggct gcactggttt
     4321 ccgccagcgg gccggagggc atgttgagca ttgccgcccc ggctcgtgac ctcaagctcg
     4381 ccaccattga gcttgaacat agtcatcctc tcgggcggtt atgggatatc gatgtcctga
     4441 cgcccgaagg cgaaattctc tcccgccgcg actattcact gccgcctcgc cgctgcctgt
     4501 tgtgcgagca aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac
     4561 tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca
     4621 aagcttgcga cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact
     4681 gaagtcaatc tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa
     4741 gatatggcgc tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt
     4801 ttcattgaat ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta
     4861 cgcccaattg gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg
     4921 cataaaggca gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag
     4981 ctcaaccagc cggtaacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggt
     5041 ctgaccgatc gcgaactgcg taccaataat tcacaactga cggcaggtca acggttgtac
     5101 cagcagcttg gccttaccgg cgcacgcggt gaagccgaag cgggttatcc actggtgatc
     5161 aaccacgcct tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca
     5221 ttgctcgata ccttgctcct actgatggcg atcaacggcg ataccaacgt tgcttcgcgc
     5281 ggtggcgagg ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg
     5341 ggcattcgaa cccccgccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa
     5401 cgaaatctca gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt tttagcacag
     5461 atttaattat ttaagcactt gataaatttg aaaatattaa ttttcggaga acccgtatgt
     5521 ctttagcaaa agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt
     5581 ttcttatccc tgtccccgac ggtatgccac cacgggcatg gcattacttc gctgtgtttg
     5641 tggcaatgat tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg
     5701 cggttactat ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg
     5761 acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg tctggctggt ttttccagca
     5821 ccacggtatg gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt
     5881 taggtcgtcg cattgccctt ttcctggtga aattcatggg caaacgcacg ctgacgttgg
     5941 gttatgcgat tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc
     6001 gtaccggggg tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc
     6061 ccaacgatcc gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta
     6121 ccagtctgag ttcgtccatg tttgtcaccg gtgcggcacc aaacgtgctg ggtctggagt
     6181 tcgtcagcaa aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc
     6241 cggttggggt tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg
     6301 aaatcacaca cagtgaagaa gtggcaacct gggcgggtga tgaactgaaa accatgggtg
     6361 cgctgacacg cagagagtgg acactgattg gccttgtatt gctcagctta ggtttgtggg
     6421 tttttggcag tgaagtcatt aatgctactg cagttggtct gctggcagtt tcgctaatgc
     6481 tggccctgca cgttgtgcct tggaaagata ttacccgcta taacagcgca tggaacacgc
     6541 tggtcaacct ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggctttattg
     6601 actggttcgc cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga
     6661 ttgtactggt tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca
     6721 ccgcaaccat gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg
     6781 aacaactgtg tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg
     6841 caaccggtcc tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc
     6901 gtcttggcgc aatcttcggg gtgatttaca tctctatgtt gctgttggtt ggctggccga
     6961 ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccgccag
     7021 gtaagcccac ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag
     7081 ttccacaccc attatgaaag cattctggcg taacgccgcg ttgctcgcgg tttctctgct
     7141 tcccttctct tctgccaacg ccttagcgtt gcaggcaaaa cagtatggcg attttgatcg
     7201 ctatgtcctg gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg
     7261 taacgaacga gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac
     7321 cgtacatggt ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg
     7381 ccgctggatg cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcgcacgc
     7441 cagccggatg tgttcatcgc cggaaaccgg attatcactg gaaacggccg ctaaactaag
     7501 tgaagtcatg ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca
     7561 cggtgcctgc tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca
     7621 agaaattaaa gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag
     7681 ccgccgtgac tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt
     7741 taagctaacg tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc
     7801 tgacgccatc aacgctccgc tttctgcaaa ctcattcttg ccacaaccac acccaggcaa
     7861 ctgtggcaaa acctttgtga ttgataaagt gggttattaa tcatctgatt tacaaaacac
     7921 gctaattcgt cttccataaa ccgtgacgat taagaattat acataatgcg ctgcattgag
     7981 gaattccctg aatgtgacgc aaatcacttc aaagagtgag caacagtcag tatcatcatg
     8041 tgagttaaac cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt tatctgcgac
     8101 actcgcagta ccgacgacat ggagtaaaaa tgtccagacc aactatcatc attaacgacc
     8161 tggatgccga acgcatcgat attctgctgg agcaacccgc ctatgctggt ttgccaatcg
     8221 ccgacgcgtt aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa gagatgccac
     8281 acgacgtggt gacaatgaac agccgggtta aattccgcaa tcttagcgat ggcgaagtgc
     8341 gtgtgcgcac gctggtgtat ccggcaaaaa tgaccgatag caatactcag ctttccgtta
     8401 tggctccggt aggtgccgca ctgctggggc tgcgcgttgg cgattccatt cactgggaac
     8461 ttccgggcgg cgttgcaacc caccttgaag tgctggaact cgaataccag ccagaagctg
     8521 ctggcgacta cctgctttaa tcccatctgc acaagcgata actcgctttg cccagaggat
     8581 gcactcgcat cctctttacg caaaatcagc aatttttcct ggttctcact gttcactttt
     8641 gctgcaaaat tattccgttt cgtttgtggg aataaccatg gcagaagcat tttacatcct
     8701 gataggattt ctgattatgg cggcgattat cgtcatggct gtcctttacc tcgaaaacca
     8761 ctcctgacga tcatattgtt ccccccggca tcgcattcac cagacctgaa accgccttct
     8821 gcgccgcctc tgcgctttgt gcaccgggta ccagaatcac cttacggcac tcctcttcac
     8881 gtttttcgaa aatctcatat ccccgggcgg cctcttcaaa cggcatatag tgggtaacaa
     8941 tttcttctgg tttcagtaat cctttctcaa ttaacggtaa taattctccc agccatgcgt
     9001 gaacgtgggt ctgtcccatt ttaaacgtca accctttatc aaaggcgtcg ccaaacagga
     9061 aaccgtgaat aaatccagcg tagacgcccg gtacgctaac aatgccgcca cgcctgaccg
     9121 ccgcaataca ctggcgcaac gctttaccgc tgctgccctc cagtttcagg ttagtcagca
     9181 ccgtttccgt ggtgctgcct ttcgcttcaa aaccgacggc gtctattact gcatccacgc
     9241 cccggtgacc tgccgtttgt tcaataattg actgtgccgg atcgctatct tcatcgaaat
     9301 taatcgggat cgcgccgtag cggtcagcgg cgaaacgcaa gcggtaggga tgatgatcaa
     9361 caacaaaaat ctgttccgca ccgagcaacc gtgcacaggc gattgtcaac aatcccactg
     9421 gaccggcacc atagactgca acgcttgaac cttgttggat ctgcgcattt tttgctgcct
     9481 gccatgccgt tggcagaata tcagaaagga aaagggcttt atcatctgaa agcaaaggcg
     9541 gtactttaaa cggccccaca ttccctttag ggacgcggac atattccgcc tgtccaccag
     9601 gaacgccacc atacaggtga ctataaccaa acaatgccgc gggagctggt atctgttttt
     9661 tattgagcgc agcgccttta cccgcattgg tattttcgca ggcggcatat tgctgcattc
     9721 gacagaaaaa acagtcgcca caagcaatga caaacggaat taccacccgg tcgccttttt
     9781 gcaaattttt tacgtccttt ccggtttcaa cgacttcccc cataaattca tgaccaaaaa
     9841 tatcgccatg tttaacctgg ggtattttgc ctcgataaag atggagatca gagccacaga
     9901 tcgccgttgc cgtaatacgc agaataatat catctgcctg ttcaatgccc ggatcgggaa
     9961 cattttctac ctgaacgtga tgtgggccgt gataagtcaa tgctttcatg caacctccag
    10021 agacagttca aaaaagagtc agtctttaag ggtagctatc acagcgggaa aacgggcggt
    10081 acctcaggtt ttgggagaat tcctggtgtt cagtgagaat atattaatca cagaaaccgt
    10141 tcgtaaggaa aaagaaccag acaaatatca gggataaata ttataatcat ggcgaatgat
    10201 ttgttcatga ttaacaggga gaaagggtta tgtataagac aatcattatg ccagttgatg
    10261 tatttgaaat ggaattgagc gacaaagctg ttcgccacgc tgaattcctc gcccaggatg
    10321 acggagttat tcatctactt cacgtactac ccgggtcagc cagcctgagc ctgcaccgtt
    10381 ttgccgctga tgtgcgtcgt tttgaagagc atctgcaaca tgaagcagaa gaacgtctgc
    10441 aaacgatggt cagccacttc accatcgatc cttcccgcat taaacaacat gtccgttttg
    10501 gtagcgtgcg ggatgaagtc aatgagttgg cagaagaact gggggctgat gttgtagtta
    10561 ttggttctcg caacccatcg atttcgaccc atctgttagg ttctaacgcc tcgagcgtga
    10621 tccgccatgc caatctgccg gtgctggttg tgcgttaatt gccagaataa atatcccgcc
    10681 ctgcccggag ccatcccggc agggcttttt aatgccataa aaaagccgcc aggtttgacc
    10741 ccggcggcta agcaattgca ggtgaatctt acttcttctt atgcagtttt ggtgcgaatc
    10801 aggtagtcaa aagcactcag agaggctttg gcaccttcgc cagtggcgat gataatctgc
    10861 ttgtacggaa ccgtcgtaca gtcacccgct gcgaacacgc ctttgacgtt ggtttcgcat
    10921 ttcgcatcaa tgataatctc acccatgcgg ttacgttcga ctgcgccttc cagccagttg
    10981 gtgttcggca gcagaccaat ctggacgaaa ataccggcca gttcgatgtt gtgaatatcg
    11041 ccgctgacac gatctcgata ttccagacca acgactttgc tgccgtcgcc tttcacttcc
    11101 gtggtttgcg cattcagaat aatgtcgacg tttttcaggc tgcgcagttt gtcctgcaga
    11161 acctggtcgg ctttcatttc tggcgcaaat tcgagcagcg ttacgtgctc aacgataccc
    11221 gccaggtcaa ttgccgcttc cacgccggag ttaccgccgc cgataaccgc tacgcgtttg
    11281 cctttaaaca gcgggccgtc gcagtgcggg cagtaggtca cgcctttggt gcgatactga
    11341 tcttcgccag gaacgttcat gttgcgccat tttgcaccgg tcgccacgat aatgctgcgt
    11401 gctttcagta ccgcgccaga agctgtttca atctgatgca gaccaccttc aactgctgct
    11461 gggatcagtt tgctggcgct ctggctgtcg atcacatcaa cgtcgtattc atcaacgtga
    11521 actttcagtg cgcctgccag cttctgccct tcagtcttcg gtacagaaat gtagttttcg
    11581 atatcaacgg tatcgaggat ctgaccacca aaacgttcgc ccatcagacc ggtacggatg
    11641 cctttacgtg cggagtaaat tgctgcagct gcacccgccg ggccggaacc aacgattaat
    11701 acgtcataag catcacgctt gttcagctct tctgccgcac gtttttccgc gcccgtatca
    11761 attttggcaa cgatttcagt caacgtcatg cggccctgac caaactcttt cccgtttacg
    11821 aacactgccg gaacgcccat cacgttgcga tcggtgattt cgttctggaa ggtgccgccg
    11881 tcaattgcag tgtgcttgat gcgcgggttc agtacgctca tcaggttcag cgcctgcacc
    11941 acgtccgggc agttatggca agagagcgag taataggttt cgaattcaaa atcaccgtca
    12001 atatggcgaa tctgctccag cagagactgc gcttctttcg acggatgacc accggtccat
    12061 agcaacgcca gtaccagcga ggtgaactcg tggcccagcg gggagcctgc aaaacgtggc
    12121 ccctggttgg aacctgggtt ggtgatcagg aaagacggct tacgcaccgg caagctgtta
    12181 tcttctttaa aggtgacttt gtctgacagt tctgcgattt cagccaacag ttccttgatt
    12241 tctgccgatt tagcgctgtc atccagcgtg gcaattaact caacaggctt ggtcaatttc
    12301 tcaaggtaag ccttgagttg agttttcata tttgtgtcga gcatgtttat ctcctgggct
    12361 ttaaacatca tcatgcaagc ggcctctgcg gctacctgga tgcagcttgc atcatggtgc
    12421 ggggaagaaa atcgggtgcc gcagcacccg aagaattagg tgaatttccg aagtatttcg
    12481 cgccggagtt ggtagcgcga aagacgaagg aaatttagat tttaccaacc aggtccagag
    12541 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    12601 aagctacgta ctgtgctgct ttgattttac gcagcaggtc agacgcgtca cggccaatgc
    12661 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    12721 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    12781 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    12841 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    12901 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    12961 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    13021 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    13081 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    13141 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    13201 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    13261 tctgaactac cctttggtat cttcagtgcc tgcctgcagg ctgaggtgtt gcccttacaa
    13321 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    13381 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    13441 gacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    13501 gaggaacttc ctgctccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaaca
    13561 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    13621 atctacctga ctccagatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    13681 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    13741 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    13801 gtctacgtgt tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    13861 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    13921 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    13981 gaatatgaat cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagaa
    14041 caaatgaaag tgttaagcgc caacgagaaa ctcatggacg atctgggggc aaatgtcaca
    14101 ccagcgatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    14161 cagaaaacgc ttaatatcat tatggggaat aaataaaaga taattcatgg atggttgaaa
    14221 tacatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctctg ccctaagtgg
    14281 ctatgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaatataat caataaaaat
    14341 atacacgcaa aaaacattga ttaagtgaat ataccatgga agaaaaatat aaccggagta
    14401 gtgtatggcc aatctctacg acttgaaaaa gttcgactta aaccttcttg tcatatttga
    14461 gtgtatttac cagcatttaa gcattagcaa agctgctgaa tcactatata tcaccccatc
    14521 agccgtgagc cagtcgttac aacgcttacg tgcgcaattt aatgaccctt tattcattcg
    14581 ctcaggaaaa ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc atcttgagaa
    14641 aaacctcaaa ggccttgaac aaacaattaa tatagtcaat aagtccgaac taaagaaaaa
    14701 ctttattatt tacggccctc agctcatttc ttgctctaat aatagtatgc tgatccgctg
    14761 cctacggcaa gatgcctctg tcgagattga gtgccatgat atcctcatgt cagcagagaa
    14821 tgttgaagaa ctgttggttc accgcaaagc cgatctggtg atcacccaaa tgccagtgat
    14881 cagccgttcc gtcatttgca tgccattgca tacaattcgc aatactctaa tttgtagtaa
    14941 taagcatccg cgaattactg acaatagcac ttatgaacaa attatggcag aagagttcac
    15001 ccaattaata tccaaatcag caggagttga tgatattcaa atggaaatag acgagaagtt
    15061 tatgaatcgc aaaatttcat ttcgtggttc ctctttgctg acaatcatta atagcattgc
    15121 agttacagat ttactgggaa tagtccccta tgagctttat aattcctacc gtgactttct
    15181 taattttaaa gagataaaac tagaacatcc gttaccttca ataaagcttt acatttccta
    15241 taataaatct tctttaaata atttggtgtt ctcccgattt attgaccgtc taaatgagag
    15301 tttttgatat ataactacag acagctacta taactttcat ctatttattc acagcgcgaa
    15361 tgaaaaataa aacactctgc tttgcagtgt ttactttata attaacagac ttatctcact
    15421 gatcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg
    15481 ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac cctaccccgc
    15541 gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa
    15601 cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga ggcgcaattc
    15661 tcttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag
    15721 ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa
    15781 tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc
    15841 gatcctgact ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag
    15901 tttgccgaat ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat
    15961 gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat
    16021 aaaccatgga ctacggccgc accaggcggt catagctggt acatttaccc catttacgac
    16081 tggaaagtgg ctgatatctg gacctggtat gcaaatcatc aaaatctctg caacccactg
    16141 tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt
    16201 ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct
    16261 gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgataat
    16321 catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca
    16381 ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc
    16441 atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac
    16501 attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat
    16561 tactggtgtc gggcattatc atttagtcct acgaaagcga agaactatca gcgttataac
    16621 gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg
    16681 atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaaagcg attaatgaaa
    16741 tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat tgcgttctgt
    16801 gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg gcaccacccg
    16861 aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca ttgtggtcac
    16921 gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg aaattggtaa
    16981 aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct gtctggaagg
    17041 tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg gacgccatca
    17101 aatcaccgcc atgtcggaaa tcgtccgtga acttagccag ttgggatggg acgataataa
    17161 aattggcaaa gagctaggaa tggacagcga cgaagtgctg cgcctgaagc aaattaacgg
    17221 cctgcaagag ctgtttgccg accgtcaata ttcccgcgcc tggacggtta aataactaaa
    17281 gctggcgcag gcgttcagct gctgccagca acgtcgattc cttcttggca aaacagagac
    17341 gaatcagttt atgtgggaag ggatcggcgc aaaacaccga cagcggaatc gccgctacgc
    17401 cgtgctcctg cgtcagccac tggcaaaact caacatcatc cagggtagaa acctcgctgt
    17461 aatccaccag caaaaagtat gtaccttcac acggtaaaat ctccagccgg ctttcattta
    17521 aagcattcac cagaatatcg cgcttctggc gataaaagtc cggtaacgca agataatgct
    17581 caggttctgc acgtagcata tcagcaagcg ccagctgtgc cggggtattc accgaaaagg
    17641 tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca acacaataac
    17701 ccactttcca gccggtcata tgataggtct tgccaaatga agaaaccgcc actgcccgct
    17761 cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag ttgatgtgct
    17821 cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg
    17881 cgaaatcagc ctgctgccag acagttgcac tggggttatg cggagtgttg aggatcacca
    17941 gtctggtgcg ctcgcttaat aatgcggcaa attcctgcca gtcaacgcga aaatgcggtg
    18001 gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg gagtaactgt
    18061 catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg gtaatcgccg
    18121 cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca tctggttgat
    18181 agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca
    18241 tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc
    18301 gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt tgctgcgcca
    18361 gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt tgtggaatca
    18421 gagggttatt tgtcattttt attggacctg acgaagttgc gtgttgaagg cactataaca
    18481 cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat aaggacaaca
    18541 caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact tttcacatcc
    18601 aggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc gcgcggtgtg
    18661 gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag cgtttggact
    18721 tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg tacagcaact
    18781 ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg gcgcactgct
    18841 cgacaccgcg aaagccctcg cccgccgtct cggtctgccg tttgttgccg ttccgacgat
    18901 cgccgccacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg ccggacaggc
    18961 gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac cggagattat
    19021 cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg cgaaatggta
    19081 tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc gactggggat
    19141 caataatgcg caggccattc gcgacgtatt gttaaacagt agcgaacagg cgctgagcga
    19201 tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg gtggatgcta ttattgctgg
    19261 tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg ctcatgccgt
    19321 gcataacggt ctgactgtcc tgccgcaaac cgagaaattt ctccacggca ccaaagtcgc
    19381 ttatggcatt ctggtacaaa gcgccttgct gggtcaggat gatgtgctgg cgcaattaac
    19441 cggggcgtat cagcgttttc atctgccgac cacgctggcg gagctggaag tggatatcaa
    19501 taatcatgcg gagatcgaca aagtgattac ccacaccctg cgtccggtgg aatccattca
    19561 ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa aagtggaatc
    19621 gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca ccgtagcgcg
    19681 cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg ggatggttaa
    19741 cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc ttcgccgcct
    19801 gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc gctaaccatg
    19861 ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa tgctatcaac
    19921 gcgctcggtc agtcccctcg ccccatcggg gagagggtta gggtgaggag gaaccctcgg
    19981 cactatgcca acattcgcca acatcccccc tcactctgac tttagtgtgc gccttttgcc
    20041 tgcgccacga tctcctcgac attttccggc attggctcat acggcgtttc tttcgccgtt
    20101 ggcttcggat ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc cagaaccacc
    20161 acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa caccagttgc
    20221 gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac
    20281 ttattagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt ctgccagcct
    20341 gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca ggcgtaacgt
    20401 tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc tgccagcatc
    20461 tggttggcaa taccaaacag cggccacaga gtgttaatgc cgcccagcgg atcgaccacg
    20521 ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ctgttgccag caggttagca
    20581 ggcagtgaat cggtccgctt caggcccgga gaaaccacgc ccagcagatc ctgcaacata
    20641 aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc ttcaaacaga
    20701 atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc gtgcaggatg
    20761 taagccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat cgactgttca
    20821 cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc ccagctactc
    20881 accacctgcg cggcagaagc gactacatcc gccgtccctg ccggagccag caccgccatt
    20941 gggctgttca tggcaaaata cacgcccgga tcgatgatac aggcagaaac cagcgccata
    21001 atcgccacga aggattccat caacatccca ccgtagccga taaagcacgt ctgcccttcg
    21061 ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc agacaccgca
    21121 ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac cgggccagtg
    21181 ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat caaaatgcct
    21241 accgccagac cgacgatagt cccgattttc aggaaggtcg agagatagtc acgcggggcc
    21301 agcagcaacc acaccggcag caccgccgcc acaaaaccgt agcccaccag catccaggtc
    21361 agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc cacccagccg
    21421 ccagagataa tggcgaaaat caggaacacc aggccaatca ctgacacttc gccaatgcgg
    21481 cccggacgca gatagcgcag gtagattccc ataaaaatcg ccagcggaat ggtgaacgca
    21541 acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat cgccagtact
    21601 gcaaggataa tgaccatgat cataaagcag gctaccagcg caatcacccc ggcagttggc
    21661 cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt agaaacaaac
    21721 agcaccatga aatcctgcac cgcaccagcg agaaccaccc cggcgagcag ccagatcatc
    21781 cctggcaggt aacccatttg cgccgccagt accggcccaa ccaacggacc tgctccggca
    21841 atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata gtccagcccg
    21901 tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac atttttggcg
    21961 atatacagcc cataaaaacg gtaagcgatc agatagatac agaccgacgc caccacaatc
    22021 cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc aaatgctccc
    22081 attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt tgttatccgt
    22141 gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtaag atttaacaac
    22201 acggtaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt taactcgatc
    22261 actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca tcccaaaact
    22321 gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac gacgatttcc
    22381 cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac cttgccttca
    22441 gacaccgggc gatgatgggt ggcgttaagt tccataccca ccacacactg cccgtcacgg
    22501 gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga cgcgccgcca
    22561 tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc ttccagcaca
    22621 tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt atcgctggtg
    22681 gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc cccagcgttg
    22741 agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca gaggcgagga
    22801 acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga atgccgagtt
    22861 taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc acccacagcg
    22921 tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc acgccgctac
    22981 ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc gatgcgccat
    23041 aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg acaatcgccc
    23101 cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac gcaccgccga
    23161 cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc gcgcccatgc
    23221 gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct aacagtcgct
    23281 gacatacctg cgcgacctgc gcagcatcgg caacatccat cacttcggtc gcaaagggat
    23341 attgctcctg agcgaacgct tgatcaaaac cggtaacttt cgctcccgcc tcaacaaacg
    23401 ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca tttttaccgc
    23461 tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga tggtcgggtt
    23521 tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg ccgccagcgc
    23581 catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga acggttcatc
    23641 ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg cggggacagg
    23701 tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca catatttcag
    23761 cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca taaacggttt
    23821 aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg catatacccc
    23881 ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg gagaacgatg
    23941 aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg tcaggcgatc
    24001 caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat cattcaacag
    24061 cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaaccg gaatattgtg
    24121 ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca tcatcgggca
    24181 gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca acgcggcacg
    24241 ttgcggttca aaggcccagt caactttatt cttagggata tcgtgagact ccggcagtgc
    24301 gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat gcgcgtgacg
    24361 ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc ggaagtgaat
    24421 ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca cgcaggaaac
    24481 gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcgcag cttttttcgc
    24541 ccatcagctc atcttccatg ctcaccagtg cggcgtagat caccgccggg tggcgcagca
    24601 gcaggttttc gatctcttcg gcagcgatct tctcaccgcc acggttgatc tgatctttct
    24661 cgcgcccctg cacggtgatg tacccctctg gatcaataga gatcagatcg ccggaacagt
    24721 aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa tagccacgga
    24781 aggtgtacgg accgcgggtc atcaggcgtc caacttcccc ttgcggcagt ggatttcctt
    24841 cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg gtatggataa
    24901 ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc atgccaaaca
    24961 cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg gcagaaagac
    25021 gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt tcaccttcgg
    25081 tcagcgcctg caaccacagg ctgactgccg gtggcaccag cgcggtgacg ttcacctgat
    25141 gtttttcaat caatgggaag caaagcgtgg cgctgggatc ggccgccaga acaaccgttc
    25201 ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta tgagccgccg
    25261 ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca aatctcgacg ctacgacgca
    25321 cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg gtggtgccgc
    25381 cggaaagctg gaaataggcc acttcatcag caggtgatgg cgtggcagta aaatcctcag
    25441 ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc agttgcacca
    25501 cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg ctaaacagcg
    25561 catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag gcgttcagtt
    25621 cactacgctg atggctgaac agcgccagca ccggcgcaac gcccagtttc agcagcgcga
    25681 aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt tcaccaggtt
    25741 taatgccctg acggcgtaaa ctacacgcga ggttatccgc cgcctgattc agctcccggt
    25801 aactcaactg tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg tggcgagtca
    25861 gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga cgggcaaact
    25921 cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt
    25981 tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt gacgacgcag
    26041 gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc gcgcagcgga
    26101 tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg ccgccaaaca
    26161 gttcgcggtc gaatggctcc agttcagcaa taacctgggt cgcggcctga tgcgggaaac
    26221 cgctcagcgc gggggttgga tgcagcagac aggccagagt cagtgcgttt tcttgcgaat
    26281 tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta atcaattgtg
    26341 gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc atcgcctgag
    26401 tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta cccgcttcgc
    26461 gatcaagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg gagctaaaac
    26521 gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg ccaccatcag
    26581 ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt tccagcaata
    26641 cgccactatc aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac accactttgt
    26701 cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca aacgtggttt
    26761 gttcaggaat tgcctggcgt tccaccacgt tcagcgactg gctgcgggta aaacggcggg
    26821 ctgaggtctg tttttcctgg cgggagaacg actgccagga ttcgggaata tacagcgacg
    26881 aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg atgccctgcg
    26941 ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg ggcgaatccc
    27001 cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg
    27061 gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag
    27121 ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa tgattatcat
    27181 ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg
    27241 caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga agcattattg
    27301 atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc tgccccctct cccttccagg
    27361 gtgagggctg gggtgagggt taatgttcgc accagtgctg gctgttcccc tcaccctctc
    27421 cccaaagggg cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac gcccataacc
    27481 ttattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa cgcccttcta
    27541 ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgctgactg gccgcgtcag
    27601 attactgaca gccgtggcac acatacactg gaaagccagc cgcagcgtat tgtttccacc
    27661 agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc cagcggcgcg
    27721 accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg gagcaaggtg
    27781 gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcaccga agccgttgcc
    27841 gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc gctggcactg
    27901 tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg
    27961 cagtcgctgt taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg
    28021 attgcacagt ttgataagca actggcagcg gcgaaagagc aaatcaaatt accgccgcag
    28081 ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa
    28141 tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg
    28201 ttaaacgcca gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggggaaaat
    28261 ctggctgctg ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca gaaagatgcc
    28321 gatgcgattt atgccaatcc actgctcgcg caccttcccg cagtacaaaa caagcaggtt
    28381 tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccacgca agtgctggaa
    28441 cgacttaagg cgctgtttta agcattaact gtcagatgct gtcacctgcg gcggcgtctg
    28501 gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa tcaacaaacc
    28561 aaaaccgctt gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac cacccagcag
    28621 cgccgcgcct atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc
    28681 taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg aactaaccgc
    28741 gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg gcatcaggcc
    28801 aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata accctggtcg
    28861 cgcactatgt gccagcttcc cgctggttaa cgcaccaata gccgcgccga gcgggatcgc
    28921 cgcgtagaga aaaccaatct gtgccgctga catctgccag ttgtcagcca gcgccggata
    28981 cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa tcccgcccac
    29041 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcacg
    29101 cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg taataaacgt
    29161 gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca ataaaccgcc
    29221 aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg ccccggcctg
    29281 cattaagttt tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg taacgccaag
    29341 cgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    29401 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    29461 aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    29521 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gcgtcatcat
    29581 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    29641 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    29701 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    29761 gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    29821 ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    29881 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    29941 tagtgctgga ggccctctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    30001 cgcgactgcc gcgcaccctt gccggtttac ttgcaggcgg cgcgcttggc cttgccgggg
    30061 cgttgatgca aaccctcacc cgaaatccac ttgccgaccc cggcttgctt ggcgtgaatg
    30121 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcgcagg
    30181 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    30241 gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    30301 cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgat gtctacgatc
    30361 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    30421 tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt actgagccgc gcgctgaaca
    30481 gtttaagcct cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    30541 taattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    30601 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    30661 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    30721 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    30781 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga tttacgtctc
    30841 tcgccgatta atcatcacct gtttgctgtt ggtttccgcc tgtgtggttg caggtatctg
    30901 gggattacgc agcggtgccg tcacgctgga aacctcgcag gtattcgccg cgctgatggg
    30961 cgatgcgccg cgcagtataa cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    31021 ggcgctgttg attggcgcgg cactgggcgt cagcggcgcg atttttcagt cgctgatgcg
    31081 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    31141 ggtagcgatg gtgctgtttg gtcaagacct gacggctatc gcgctggcag caatggtggg
    31201 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggca tcgacacctt
    31261 tcggttgatt attatcggta tcggcgttcg tgccatgctg gtggccttta atacctggct
    31321 gttgctgaaa gcgtctttag aaacggcgct aacagcgggt ttgtggaatg caggatcgct
    31381 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    31441 tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg ataccgcgtg
    31501 tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg cagtggtgct
    31561 taccgctgcg gcaaccgcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    31621 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    31681 ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact
    31741 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    31801 ggagtctcgc aaaaaatgaa cgaatctgta gcccgtttgc gcggcgaaca gttaaccctc
    31861 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga tggtcacttc
    31921 acggcaatta tcgggccaaa tggctgcggt aaatccactt tactgcgtac tttaagccgc
    31981 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    32041 agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccaggcgat
    32101 atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc atcaaccgct atttacccgc
    32161 tggcgcaaag aagatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    32221 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgcgc gtggatcgcg
    32281 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    32341 atcagtcacc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    32401 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    32461 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    32521 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaacg
    32581 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    32641 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    32701 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    32761 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    32821 ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa ttcaccgttc
    32881 agttccgcaa cgtcagtgac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    32941 ccgagagaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    33001 tttttaattc ccgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    33061 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    33121 ttgatttcca gtttattacg gacgttttct agtgactctt tcaccaccaa cgcagagata
    33181 taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc ggtaaaactt
    33241 agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt gtcatcaacc
    33301 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    33361 gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    33421 accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat tttgatatcc
    33481 agatccagca ctcgaagctt agtgaatgtt ttctccagct cttgccactg aacaggttct
    33541 ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga gatcagcaag
    33601 cctgcgcagg caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg ccataaaact
    33661 gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagga
    33721 aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa cctgaaaatg
    33781 agtccaatag gggaaatctc cacggcgctc actttaccag ttggtgataa ttatccgaag
    33841 ctgaagtttg taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc
    33901 atcaaaccta taaatcaaac aaataaaaaa acccataatt attttctgta attatgggtt
    33961 tatagatatt attaatttac ctgtttagcg ttgcgcgaat aatcggccca atttttacaa
    34021 acgcccctgg agagataata tccacatgcg cacaatcctg acgatagata tccagctccg
    34081 caatccacgg cgaccaggca cgttcgggac tcataccttc ctgaagtgtg cgctcagcaa
    34141 caaataacgt tgcttttcca tcaaacggaa cgctatgggc ggtggttaac aagcgcacag
    34201 catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt ccctgctgtg
    34261 ccgccaggaa ggcctcgcgt tcgcggttaa tctccgccag cacttccggg tccagaccat
    34321 tagcctcttt ttcctgccag ttttgcgtct ctggcggcca ggtatccagc aaaccaagaa
    34381 atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc
    34441 caagggaata ccccagcagg taataagggc cgtgcggttg ttgctcaagt aacgttgcca
    34501 gatgcgcttc gcagacttca tccaggtttg tcgctgtctg catgggtcca tgagggcgcg
    34561 gcgactgaat gccaataatc gaccattgtg gatcgagata acgcgagagc acgctaaact
    34621 gccaggcaaa accggacgcc ggatggaaac aaaacagcgt cgggccatta ccttcacgca
    34681 acggcagaat ggtttcgaat cccatgcgcc gggagctgtc ctcttcacca tcaataatcg
    34741 ttgccagttt ggcgacggtt gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa
    34801 actgtcgact taactgcgct gctagtttca ttgccagtag cgaatgaccg cccagcgcaa
    34861 agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga
    34921 taatcgtttc actgcccgct ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca
    34981 acggtaaggc tttgcgatcc agcttgccgt tggcgctaag cggcaactgc ggaagttgca
    35041 gcagaacgac cggcaccata tgcggcggca atgtttcgcg aagctgtgcc tgtaatgcgc
    35101 tggtatccaa cggcagtccc gattgcgaca ccagatagcc caccaactga cgcgcatcac
    35161 caccggtggc tgccgcctgg ttaatcacac aggcgtgggt aactgcctgt tcgacatccg
    35221 gcagcgcctg catcacgcga tcgatttcgc ccagttcgat acgctgcccg cgaattttta
    35281 gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag cgggcgacgt
    35341 ctccggtacg atacatccgt tcacctgggg caaaaggatc ggcaataaaa cggctggcgg
    35401 tcagatcggg tcgtccaaga tacccctgcg ccagttggat gccggtgaga tagagatcac
    35461 ccgccacacc cggcggcacc ggatgcatca tcgcatcaag aatacgcaga cccgtattcc
    35521 ataccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc tcgccaaaag
    35581 ccggatacca gctgacatct accgccgctt ccgtcgggcc atacaggtta tgcaacggcg
    35641 cgccggttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca ccactacaga
    35701 aaacctgttt caacgtcgcg caattctggc gagcggtttg cggcgtcagc gatgcaacaa
    35761 atgccgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg gcaaagaatt
    35821 gctgcatagc gagcgggtca cggtgcgctt ccggttcagc catcaccagt tttgcccctg
    35881 cgataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc gttttttggg
    35941 caacgacatc ttcgcctgta agcgggtagt gattttgcat ccataacagg cggttaacga
    36001 tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag gtaaagatga
    36061 tataagccgt gtggtgcggt tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg
    36121 gggcgttata gcaaaggctt gttaaattgg gaacatcgct aaagcgcggc agttgatcgt
    36181 cggtggtaat taacagcgac ggacgcgcat cttccagcat cattttcagg cgatcgtccg
    36241 gatagccggt atccagcggt agccaggccg cgccagcttc aactattgca tggagtgcca
    36301 gggtcaaaaa gaccgagcgc ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc
    36361 gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttcccga tagctgaaca
    36421 ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcttgt tctgccacca
    36481 gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc gccagctgcg
    36541 catactcacc tggcagcata atatcgacat cgccgcacaa cagcgccgga tccgcggcga
    36601 actgggcaat cagcattttc aggcgttcag catgctggat taacgttggc tcatcgtaac
    36661 gctgtttatt ggcgaggatc tcaatactca aatcaccgtg ttcatccggg aacagggcca
    36721 gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgtctga acaccaggaa
    36781 tatccagttg gtaatcaaat accttgatat tgagtaccgg accgaacagc ggttcctctc
    36841 ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga tgacgacgca
    36901 tttttttcag ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa
    36961 tgtgaatacc caacggtaaa acgttgagca cgggtccgct agccgtcagc gccgccgagc
    37021 ccagtcgacg cataaagata aatccggcgg catagtccat gcgattgcac aatcgcccca
    37081 gccacaaggc tgccagcgca agggctaaat cggtacgctg cacacctgaa agttgcgtag
    37141 ccagctggcg gaattccccg tcggtaaatt ccagtttcag gcgcagaata tctgccgaag
    37201 cgctgcgccc cgctaaaggt gccggagaaa gtgacgcggg cggcggcagt tgacgacgct
    37261 gttctgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat tgctggtact
    37321 cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc
    37381 atgtgcagta aatattggcg atctggcgag taatggccgg gaaactgaag ccatcgacca
    37441 gcaaatggtg ataacgctga taccagtacc agcggttatc cgcaacatgt atcagctgat
    37501 gaaagaccag tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta
    37561 atgcctgcgc agtaccgtgc ggatcaatat tggttcgcag gtcgataatt tctggcagtt
    37621 cgaatatcag cgcatcatcg acccactgcc agacttcgcc gttatcttcc gtaaaacgca
    37681 tccgcaacgt atctgcttgc gctagtccga caaccaccgc gcgggccagt aatggcgcat
    37741 caacatctcc agttaactca acgtaatgcg ccacgctcca ggcggagggt aaatccgaca
    37801 gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg
    37861 tgcctcctgc aactgggtga aattggccgg tgtcagagta cgccagtgga cttccagcca
    37921 ctgctggcag gacgcctgtg actgcggctg acacacaatg tcccagcctg ccggtaaggc
    37981 gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc
    38041 ctgtggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg gaaaagtggt
    38101 tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg
    38161 ccgtcaacct gacgccagaa aatggattct tttatggggt gtaattgcgc atacagcgcc
    38221 tgattggccc gcataatcat cggctcgcga atacccgctt ccagcacaat gcgcagacct
    38281 tcggcgctaa cttcaccagc tttaagcttt tcaagtaaca cgccctcttg ctgcccgccc
    38341 cgatgcggcc accagtacga tcctgactgg cttaatacac agccaaagcg ttcaggccag
    38401 tgcagtccgg catacagcgc ggacagccca ccaaaactct gcccggcgac cacggtgcga
    38461 tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg gtaataactc ttgctgtact
    38521 gccagccaga aatccgcatt acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg
    38581 tcgatcaaca catacacggc gggaggaagt tgctgacgat gggtcagcga agtcagcaca
    38641 ggccagacgg gcatactttg cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt
    38701 tcttctgctg ttgcatcgcc ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt
    38761 tcacttttcc agataatttc tttggctgct atttctggcg cttgcggaca atcccatccc
    38821 ggttgcagag gcgcttgcgg catttcgagt gcagaaacag cgtgccctcg cccgcctttc
    38881 cagctttgtg gattcagcgg atcggctatt gcctggggta atagttttcg ccagccttca
    38941 cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata
    39001 aagcagtagc tgccgcgcca gttggcattg agttgtgtcg tccactgcca gacatcagta
    39061 cctgcaattc gctgcatcga ctggggctgg ctgttctgat gatgatcggt cacaccagtg
    39121 atgtagatcc atacgcgctt tatcgtcgag tattcttcag aaccttgggg atcacgccac
    39181 cagaaagtga cctcaaacat ttcgtcattc agacgctgcc attccgggcc atgtttcgac
    39241 tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca tgtttactgt
    39301 gcaatttttc attgattgca gaaatatatt gataatatta ttgataacta tttgcatttg
    39361 caatagcgta atggcgcgcc gtgggaagcg cggacattta ttaaccaact gcactgcgtg
    39421 tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc
    39481 cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga agattcattc
    39541 cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag agccgaccga
    39601 tactcctgtt tcacatgacg ataccattgt cgttaccgcc gccgagcaga acttacaggc
    39661 gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt
    39721 gtcggagatc atccgtacca tgccaggcgt taacctgacc ggtaactcca ccagtggtca
    39781 gcgtgggaat aaccgacaga ttgatattcg cggtatgggt ccggaaaaca cgctgatttt
    39841 gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg
    39901 cgatacccgt ggtgatacct cctgggtgcc acctgaaatg attgaacgta ttgaagttct
    39961 gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat
    40021 taccaaaaaa ggcagcggtg agtggcacgg ctcctgggac gcttatttca atgcaccaga
    40081 acataaagag gaaggtgcca ctaaacgcac caacttcagc ctgaccggtc cgctgggcga
    40141 cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat
    40201 caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg
    40261 cgaaggggtg atcaataaag atattaacgg cgtggtgcgc tgggacttcg caccattgca
    40321 atcgctggaa ctggaagcgg gttacagccg ccagggtaac ctgtatgcgg gcgataccca
    40381 gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca accgcctgta
    40441 tcgccagaac tacgcgctga cctggaacgg tggctgggat aacggcgtga ccaccagcaa
    40501 ctgggtgcag tatgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac
    40561 cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc tcgatgacgt
    40621 gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaga cgctgacgct
    40681 gggtacggag tggaatcagc aacggatgaa ggacttgagt tccaataccc aggcgctgac
    40741 cggggcgaac accggcggtg ctattgatgg tgtgagtgcc accgaccgta gcccgtattc
    40801 aaaagcagaa attttctcgc tgtttgccga aaacaacatg gagctgactg acagcaccat
    40861 cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
    40921 gctgaacata tcgcaaggtt taggcgatga cttcacgctc aaaatgggca ttgcccgtgc
    40981 ttataaagca ccgagcctgt accagactaa cccgaactac attctctaca gtaaaggtca
    41041 gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga
    41101 aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacggctggc tggcgggcgt
    41161 cacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtgg ctgtagggca
    41221 aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcag tggttgaagg
    41281 tctggaagga tcgttaaacg taccagttag cgaaacggtg atgtggacca ataacatcac
    41341 ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca tcccggagta
    41401 cacgttgaac tcaacgctga gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt
    41461 cacctggtac ggtaagcagc agccgaaaaa atacaactac aaaggtcagc cagcggttgg
    41521 cccggaaacc aaagaaatca gtccttacag cattgtgggt ctgagcgcga cctgggatgt
    41581 gacgaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg
    41641 tgcgggtaat gcccagacca cgggcgatct ggcaggggcc aactatatcg cgggtgccgg
    41701 ggcgtatacc tataacgagc cgggacgtac gtggtacatg agcgtaaaca ctcacttctg
    41761 atgctaacgt cagattgttg acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc
    41821 cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccatg taggcctgat
    41881 aagcgtagcg catcaggcag tttagcgttt gtcatcagtc tctaatatgg tcgacatgaa
    41941 aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg agttcgatcc
    42001 ggcgaatttt tgtaagcagg atttactctg gctgccgcat tacgcgcaac tgcaacacgc
    42061 tggacggaag cgcaaagccg aacatttagc cgggcggatc gctgcaattt atgcgctgcg
    42121 ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaaccgg tgtggcctgc
    42181 ggaggtatac ggcagtatta gccactgcgg ggctacggca ttggccgtgg tttcccgtca
    42241 accgattggc gttgatatcg aagaaatttt ttctgcgcaa acagcgaccg aattgacaga
    42301 caacattatt acaccagcag aacacgagcg actcgcagac tgcggtttag ccttttctct
    42361 ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg agatccaaac
    42421 tgatgcaggt tttctggact atcagataat tagctggaat aaacagcagg tcatcattca
    42481 tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaaaatag tcataactct
    42541 gtgccaacac gattaattga caacatctgg tacgattcgc ccgcagccat cactgaccgc
    42601 gggcgaaagt gtaaagcagg tgccttacca ctctgacctg acaaccggat atgcggggat
    42661 tgctccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt aaactcaata
    42721 ttacgttcct ttacggtgaa ctcacacagc gagtctccga ccagaagcgt aaatcccagt
    42781 accgtcaaac acagcactat tactgccaca agggcatatt tcgtcagcat gtttatattg
    42841 cctccatgcg aagaagggac taccatccga gttgttgagg ctcggagtgg cagccccagg
    42901 attgatagaa atatctcctg gggcttcatc tttccagacc tcagagttaa cctgaaaccg
    42961 gaaagcttca ggcacccgcc gttatcttac ccttcctcaa caaaatatct gtttatttat
    43021 acagtgaatc ctgtttcgga cttctgtcgg caaaactcat cattgctctt aaaacgaaat
    43081 cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc aggtgcctta ccatcctgac
    43141 ctgacaaccg gatatgcggg gattgctccc cgcacaacgg ctacttcttc ggttcgtaag
    43201 cgagaacagc cttaaactca atattacgtt cctttacggt gaactcacac agcgaatccc
    43261 cgaccagaag cgtaaatccc agcaccgtta aacacagcac tatgactgcc gcaagggcat
    43321 atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc caaattgttc
    43381 aggtttgggt aggtagcccc agggttgata gaaatatcgt cctgggactt catctttcca
    43441 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgcagttatc ttacccttgc
    43501 ttagcaaaat agctgtttat ttatacagta agtcctgttt cggacttctc actccccatt
    43561 tgatcaactg aagctgaacc cgtttttttc tgcatgcctc aatcgtcaaa attcttcgcc
    43621 accagccagc cgtgccataa cttgtcacat tgcaaaagta gccaaattgc ttcagtcacc
    43681 atcaccaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct ccgacttcta
    43741 tactgaatag aaaacgccaa cttaagagaa acctatgcca ttacccgatt ttcatgtttc
    43801 tgaacctttt accctcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga
    43861 cttaagccag gactcttcaa tgctgattga cgcggttaaa aatgagatca cggccggaga
    43921 ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat
    43981 caaccaggct gccgggcagt tttcagcgat gcagaaagtc gtattgcagg cagccgcaga
    44041 ccatcatctg gaaatttgcg gcggtggcac gcacccgttt cagaaatggc agcgtcagga
    44101 ggtatgcgac aacgaacgct atcaacgaac gctggaaaac tttggctatc tcatccagca
    44161 ggcgaccgtt tttggtcagc atgtccatgt tggctgcgcc agtggcgatg acgccattta
    44221 tttgctgcac ggcctgtcac ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc
    44281 gtatatgcag ggaacggata cgcgttttgc ctcctcacga ccgaatattt tttccgcctt
    44341 tcccgataat ggcccgatgc cgtgggtcag taactggcaa caatttgaag ccctgtttcg
    44401 ctgcctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc
    44461 cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca
    44521 cgcggtaaat atggcgggat taattcaggc caccgcccac tggttactga cggaacgccc
    44581 gtttaaacat caggagaaag attacctgct gtataaattc aaccgtttcc aggcctgccg
    44641 ctatgggctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga
    44701 agataccttg cgattgctgg aaaaaatcgc cccttccgca cataaaatgg gtgcatcgag
    44761 cgcgattgag gccctgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg
    44821 cgatttcgtc gccgatggtg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg
    44881 ggccggtgac taagtaggga attgccttac agaagaatat ccctgacaat ggtgttttaa
    44941 cttacattta acaatgttat gaaacaccct ttagaaacct tgactaccgc agcaggcatt
    45001 ttgctgatgg ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg
    45061 gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta cactttattg
    45121 ttttgtctgt ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg
    45181 caacgctggt tctcgctggc ggattaaacg tcgattatcg ccgtacttcg cggcatactt
    45241 tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc
    45301 gaaacgcctg gcccttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt
    45361 cgatgttttt aaaattggcg gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc
    45421 ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaga tatacccgag
    45481 cattaagcca gggtatcaca tgaataaaaa gcactggatt tcggtgtatc ccggcgagga
    45541 aatctccgaa gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg
    45601 tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcggaaatc tatagcgcat
    45661 ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattactttc
    45721 acatggagtc tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt
    45781 tgatgccagt aaaaaactta ccccggaaca ggccgagcag atcaaaactc tcctgcaata
    45841 cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg
    45901 taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacgaac gtaaaatact
    45961 tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa
    46021 gctggttgtt gaccaggaag atgctgatgg ccgctttgcc acgccggaag cgaaagccgc
    46081 gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt aaagatctgc atgatgatgc
    46141 agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc
    46201 gctgggtctg gacgcggtac ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt
    46261 tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt
    46321 tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacatca ccttaaccga
    46381 agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt
    46441 gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag
    46501 gatttaatat cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg
    46561 gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg
    46621 gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc
    46681 attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat
    46741 attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc
    46801 gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt
    46861 ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag
    46921 gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt
    46981 cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggtt
    47041 tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg
    47101 aaactgggcg actggctgga gatgccgaaa tacggcgcgg atggcgcggt gatcgatatt
    47161 gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg
    47221 tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt
    47281 attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg
    47341 caacgtctga ataaagcgca cttgttaaaa ccttatttaa ccagccgcca tcaggaaatt
    47401 aatgagtgga atcgccagca aggttctacg gagtcgatat taaacctgcg ccgaatgacc
    47461 aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt
    47521 aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag
    47581 atctacgcgt ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata
    47641 ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc
    47701 ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca
    47761 tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac
    47821 gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca
    47881 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcaatgcaaa
    47941 gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca
    48001 tcgctgcacg aaaacgcaga tgcgccagac agatcataat ccagttcaac agcagcgttg
    48061 ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaata
    48121 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc
    48181 tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc
    48241 tgttagagta aaccccgctg ttatacactg acagcgatgc taccagaatg acgaagttca
    48301 gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaac gggctactgt
    48361 tggatttcac ttccacccac ggatagagcg ccagtaaaac caccagtgaa ccgatgtaaa
    48421 acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc
    48481 gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
    48541 ccagcgacaa aatcagccca ttccagccgg tggcaaagaa accaccgtag cgccagaggt
    48601 tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
    48661 tcataccgat gatcgccagc actttaatca gcgcaaacca gaactcggtt tcaccatata
    48721 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
    48781 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
    48841 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
    48901 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct
    48961 cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
    49021 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
    49081 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
    49141 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
    49201 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
    49261 cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat
    49321 cagcttatac gccgccggga taataaacag cgacagcaaa ggtgcggtga tcatgccgcc
    49381 aatcatcggt gcggcaatcc ggctcatcac ctctgaacca gcccccgttc cccacagaat
    49441 cggcagcaga ccggcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac
    49501 cgcgccgtga tataacgcct catccagctt ctgctcgctg aatgtttgtg gattattcaa
    49561 cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc
    49621 ggcgaccccg gcgagggcga taaagccagt gcccgtcgcc acggaaagat gaaagcccat
    49681 ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa
    49741 cgcttcgccc acccgacgga acgccagata caacagcacg aagataatca tcaacgtcat
    49801 cggcaccatg agtttaagct tatggttggc gcgctccagt agctcgaact gcccggagaa
    49861 tgccacgctg gtgcccggtt ttaactgcac tttttcagct atcgcttttt gcaaatcgtg
    49921 aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc
    49981 attctcggtt ttcagcattg acggtccggt agagacttta atgtcggcca cgtctgccag
    50041 ggtgatttgc tgcttcatcg gcgtcaggat cggcaactgg cgcagtgcct gcggactatc
    50101 gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt ccaccgtttc
    50161 gccaaccatc gccccgccca ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat
    50221 accgtaacgt gcggcttttt cacggttaat ctcaacgttg atatagcgcc caccttccag
    50281 acgctcggct agagcagaag ccacgcctgg caccgttcgc gctacttctt caatttgctc
    50341 agccatcgcg tcgatatccg ccagcacagt gccggaaact ttaatgccga tggggctttt
    50401 aatgccggtt gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag
    50461 ccccggcaga cgcacggtgt tatccagttc ctcaatgatt ttgtccattg tcatgcctgg
    50521 ccgccactgc tcctgcggct taagctggat ggtcgtttct accatctcca gcggagcaga
    50581 atcggtggcg gtttccgctt tcccggtttt gccaaatacc cgcgccactt caggtacgct
    50641 cataattagc ttgtcggttt tttgcagcat actcgccgcc tctgctgcgg aaatccccgg
    50701 cagcgtcgat ggcatataca acaggtcgcc ttcattgatc tgcggtaaaa attccccgcc
    50761 aactttattg agcggccaga gaaccgtcag caccgaaagc gctgccacca acagcgtggt
    50821 tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt
    50881 gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtaaccca tcaggatagg
    50941 gatcactacg atcgccagca gcgccgcacc cgccatcgca tacgttttgg tgaacgccag
    51001 cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa acgacaacgt
    51061 gataatcagc agactgataa atagcgccgg acccacttca acagatgcat cggtgatcac
    51121 ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc actcttccag
    51181 ccgtttatgt gcattttcga tcatgacgat agcggcatcg accatcgccc cgacggcaat
    51241 cgcaatgccg cccaacgaca taatattggc attcagtccc tggaagtgca tgacaataaa
    51301 agcaatacac aaccccagca gcaacgaaat aatcgccacc agcgccgagc gcacatgcca
    51361 gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact tgccgctgag
    51421 gttgtcgatg gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta tctccacgcc
    51481 ttccggcaga ctacttttca gcgtttccag tttgtccttc acggcggcga tcacttctcg
    51541 ggcgtttttg ccggatcgca ggatcaccac cccgcccgcc acttcgcctt cgccgtttag
    51601 ttcggcaatg ccccggcgca tctccgggcc aatctggacc ttcgcaacat cgcgcagata
    51661 aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg
    51721 cagatagccg ctggcgcgca ccatatattc cgcttccgcc agttcgatcg acgaaccgcc
    51781 cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatactg
    51841 cgccaggcgc tggggatcga taaccacctg atactctttc accacaccgc ccaccgacgc
    51901 cacttccgca acgtcaggga tggttttcag ctcatatttg agaaaccagt cctgcaatga
    51961 gcgtaaatcg gccagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca
    52021 gccaacaccc gtggcatctg gtcccagctc ggcgctgact cccgcaggca gcttaccctg
    52081 tacctggttg aggtactcca gcacccgcga acgcgcccag tacggatcgg tgccatcttc
    52141 gaaaatgaca tacacataag agtcgccaaa ctgtgagaaa ccgcgcacag tcttcgcgcc
    52201 aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgtgg
    52261 tgcctgaccg ggatagctgg ttttaataat aacctgcaca tcggagagat ccggcagcgc
    52321 atccactggc gtattaatga tggtccaggt gccccagatg ctcagaaaca acgcgcccat
    52381 cagcaccaaa aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc
    52441 cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg
    52501 gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc cgccagacca
    52561 gagcgtaatg cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag
    52621 cgcccgtcgg catcaacggt aatcacccgc tgttcgctgc cggtatcaat cagcgcttgt
    52681 gacggaatga gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc
    52741 ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg
    52801 gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc cggtcgcgcc
    52861 ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgatgga ctccgggatc
    52921 gcagcagtga cccacaccgg gtccataccc tgaattttcg cgaccacgtt atctttggcg
    52981 atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg cgctttgagc
    53041 gtaaagcgag tctggatttt ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt
    53101 cccgccagtc gcagccgctc aagaatgcct tcagtctggg tcgccgtacc gccggtttcg
    53161 cgcagcagta aatactcact ctgcgcttcc acccagtcag gaatggtcag gtcgagaagc
    53221 ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttgtc gataaacccg
    53281 gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt cgccgggaaa
    53341 ctctgggcaa aagtcagcgg cccgcgcgtg acggtagccg ttttcacccc cagattctgc
    53401 gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc atatttcggc
    53461 accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt attggggtac
    53521 atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc tgcgggtggt
    53581 tcagcctttg caaaccaggt aaaacctgcc gcagaaataa taccgcccgc gatcatgctg
    53641 ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct gactttaata
    53701 tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt gtcgccagtt
    53761 ttaatttcac tcattttcgt ctgcggagtg atggtaaagc gcatggtcat ctccggccag
    53821 ttcacggcag caatcggatc gtgatggatg gtgatttttt tgctttccag atcaataccc
    53881 tttaccacgc cagtggcgct aataacctgc ggttgtactt cgctcatggt ttcatgatga
    53941 tgttcgttag cctgggcatt aaagccaata acggtaaaca gactgaacat tgcgacttgc
    54001 agtgcttttt tcatttattc tctcctggag ttaaaagtca tactctaaat aattcgagtt
    54061 gcaggaaggc gacaagcgag tgaatcccca ggagcttaca taagtaagtg actggggtga
    54121 gcgaacgcag acgcagcaca tgcaacttga agtatgacga gtatattgct gtcaaccgcc
    54181 acctaaggcg gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa
    54241 agtttgtcgg gttgcaaata aagaacgctc ggcatccagc acttccagat aacttactgc
    54301 gccgtgctga tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg
    54361 ctgctgggcg ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc
    54421 tttaaaggcg ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc
    54481 ggcgatatcc agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg
    54541 aataaaattc cacatcccgc tgctggcgtt aaataatgac gacagatcgc tgctggcggt
    54601 tgatattccg ctggtcagcc ttatagatgg gaaaaatgcc gcgcgtgcgg ccccaatatt
    54661 ggcattagcc gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat
    54721 ttgcgacgac aatcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg
    54781 cgcttgcggc agcttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc
    54841 cagttccccc tgacgtttag cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg
    54901 ttccagcgcc agaacattac tgctacctgt caacagttgt ttttcgacaa acgcatatga
    54961 ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ctagctgctg
    55021 attgaaataa ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgggcttc
    55081 ctcagtggct aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa
    55141 atcgagatca aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc
    55201 tttaagatta ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc
    55261 atcggtcaga cgatattgcg cccgcgcttc ctgcactttc agcgtcgcca tgcgcaaatc
    55321 ccggttattc accagcgcct cgctaatcag cgtctttacc tgattatcaa caaaaaaggt
    55381 gcgccagccc acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga
    55441 gaactgctgc ggcacgggca ttgccggacg ctgataatcc ggtgccagtg aacaaccggt
    55501 tagcgcaagg gccacacaaa atggcagaag tttacaagga gacataggct cataatttct
    55561 ggtgatttta taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg
    55621 acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc tagaatagcc
    55681 cgggcaacat gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga
    55741 gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg
    55801 ctgaatggct accatctggc gatgaccggt gattatgatc tgataatcct cgatattatg
    55861 ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa taaagggatg
    55921 ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg
    55981 ggggcagatg actaccaggt gaaaccattc gcttttgctg aactgctggc gcgggtgcgc
    56041 actctcctgc ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg
    56101 atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt
    56161 aaagagttta ctctgctgga gtttttcctc cgccatcagg gcgaagtgct gccccgctcg
    56221 cttatcgcct cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgta
    56281 gcggtgaagc ggctgcgcgg caaaatcgac aacgactttg agccaaagct aattcagacc
    56341 gtgcgcggcg tgggttacat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc
    56401 gttttcgctg gcaacccgcc tgaccttttt tatcagcctg gccaccatcg cagcgttttt
    56461 cgcctttgca tggatcatga tccactcagt aaaagtgcat tttgccgagc aggatattaa
    56521 tgatttaaaa gaaattagcg ccacgcttga acgggtactt aatcatcctg acgagacgca
    56581 agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatctc
    56641 cctggcagat agccacggca aaacggtgta tcactccccc ggtgcgcctg atatccgcga
    56701 gtttgcacgt gatgccatac ccgataaaga cgcgcggggt ggcgaggtgt atctcctttc
    56761 cggcccgacg attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg
    56821 gatgattaac ttgtcggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat
    56881 cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat
    56941 gaccgcatcg gtaatcagca tcctgatcgt ctttatcgta ctgttggcgg tacataaagg
    57001 tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga
    57061 cgttcgcctt gacccgcaga ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa
    57121 ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat
    57181 cgctcacgaa attcgcacgc cgattaccaa tctcatcacg caaacggaaa tcgccctcag
    57241 ccagtctcgc agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac
    57301 gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct
    57361 aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt
    57421 cgaggcgtta gcggaagatc gcggcgtgga gttgcgattt gttggcgacg aatgtcaggt
    57481 tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg
    57541 ttatacgccg cccggagagg caattgtagt gcgctgccag acggtcgatc accaggtgca
    57601 agttaccgtc gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga
    57661 ccgtttctac cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct
    57721 ggcgatagtg aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc
    57781 gcgggggaca aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc
    57841 agggcgtggt gcatcacctc ctggtattgt tgatcaacaa attaga
//
LOCUS       DAZUFB010000019        57014 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZUFB010000019 DAZUFB010000000
VERSION     DAZUFB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.019"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(186..449)
                     /gene="cedA"
                     /locus_tag="ACHY3M_001354"
     CDS             complement(186..449)
                     /gene="cedA"
                     /locus_tag="ACHY3M_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFS4316295.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            632..2893
                     /gene="katE"
                     /locus_tag="ACHY3M_001355"
     CDS             632..2893
                     /gene="katE"
                     /locus_tag="ACHY3M_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFS4316296.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3152..3910)
                     /gene="chbG"
                     /locus_tag="ACHY3M_001356"
     CDS             complement(3152..3910)
                     /gene="chbG"
                     /locus_tag="ACHY3M_001356"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFS4316297.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3923..5275)
                     /gene="celF"
                     /locus_tag="ACHY3M_001357"
                     /gene_synonym="chbF"
     CDS             complement(3923..5275)
                     /gene="celF"
                     /locus_tag="ACHY3M_001357"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4316298.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5380..6222)
                     /gene="chbR"
                     /locus_tag="ACHY3M_001358"
     CDS             complement(5380..6222)
                     /gene="chbR"
                     /locus_tag="ACHY3M_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFS4316299.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6230..6580)
                     /gene="chbA"
                     /locus_tag="ACHY3M_001359"
     CDS             complement(6230..6580)
                     /gene="chbA"
                     /locus_tag="ACHY3M_001359"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFS4316300.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6631..7989)
                     /gene="chbC"
                     /locus_tag="ACHY3M_001360"
     CDS             complement(6631..7989)
                     /gene="chbC"
                     /locus_tag="ACHY3M_001360"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFS4316301.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPVLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8074..8394)
                     /gene="chbB"
                     /locus_tag="ACHY3M_001361"
     CDS             complement(8074..8394)
                     /gene="chbB"
                     /locus_tag="ACHY3M_001361"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFS4316302.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8693..9031)
                     /gene="osmE"
                     /locus_tag="ACHY3M_001362"
     CDS             complement(8693..9031)
                     /gene="osmE"
                     /locus_tag="ACHY3M_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFS4316303.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9233..10060
                     /gene="nadE"
                     /locus_tag="ACHY3M_001363"
     CDS             9233..10060
                     /gene="nadE"
                     /locus_tag="ACHY3M_001363"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4316304.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10290..11177
                     /gene="cho"
                     /locus_tag="ACHY3M_001364"
     CDS             10290..11177
                     /gene="cho"
                     /locus_tag="ACHY3M_001364"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFS4316305.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11137..11712)
                     /gene="ves"
                     /locus_tag="ACHY3M_001365"
     CDS             complement(11137..11712)
                     /gene="ves"
                     /locus_tag="ACHY3M_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFS4316306.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11915..12400)
                     /gene="spy"
                     /locus_tag="ACHY3M_001366"
     CDS             complement(11915..12400)
                     /gene="spy"
                     /locus_tag="ACHY3M_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFS4316307.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12730..13698)
                     /gene="astE"
                     /locus_tag="ACHY3M_001367"
     CDS             complement(12730..13698)
                     /gene="astE"
                     /locus_tag="ACHY3M_001367"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFS4316308.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13691..15034)
                     /gene="astB"
                     /locus_tag="ACHY3M_001368"
     CDS             complement(13691..15034)
                     /gene="astB"
                     /locus_tag="ACHY3M_001368"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFS4316309.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPIRELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15031..16509)
                     /gene="astD"
                     /locus_tag="ACHY3M_001369"
     CDS             complement(15031..16509)
                     /gene="astD"
                     /locus_tag="ACHY3M_001369"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFS4316310.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16506..17540)
                     /gene="astA"
                     /locus_tag="ACHY3M_001370"
     CDS             complement(16506..17540)
                     /gene="astA"
                     /locus_tag="ACHY3M_001370"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFS4316311.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17537..18757)
                     /gene="astC"
                     /locus_tag="ACHY3M_001371"
     CDS             complement(17537..18757)
                     /gene="astC"
                     /locus_tag="ACHY3M_001371"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFS4316312.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19203..20009
                     /gene="xthA"
                     /locus_tag="ACHY3M_001372"
     CDS             19203..20009
                     /gene="xthA"
                     /locus_tag="ACHY3M_001372"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4316313.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20176..20886
                     /gene="ydjX"
                     /locus_tag="ACHY3M_001373"
     CDS             20176..20886
                     /gene="ydjX"
                     /locus_tag="ACHY3M_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS4316314.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20891..21568
                     /gene="ydjY"
                     /locus_tag="ACHY3M_001374"
     CDS             20891..21568
                     /gene="ydjY"
                     /locus_tag="ACHY3M_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFS4316315.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21583..22290
                     /gene="ydjZ"
                     /locus_tag="ACHY3M_001375"
     CDS             21583..22290
                     /gene="ydjZ"
                     /locus_tag="ACHY3M_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS4316316.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLASIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22290..22838
                     /gene="ynjA"
                     /locus_tag="ACHY3M_001376"
     CDS             22290..22838
                     /gene="ynjA"
                     /locus_tag="ACHY3M_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4316317.1"
                     /translation="MGLPPLSKISFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22848..24014
                     /gene="ynjB"
                     /locus_tag="ACHY3M_001377"
     CDS             22848..24014
                     /gene="ynjB"
                     /locus_tag="ACHY3M_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4316318.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNRRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23987..25522
                     /gene="ynjC"
                     /locus_tag="ACHY3M_001378"
     CDS             23987..25522
                     /gene="ynjC"
                     /locus_tag="ACHY3M_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4316319.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25522..26175
                     /gene="ynjD"
                     /locus_tag="ACHY3M_001379"
     CDS             25522..26175
                     /gene="ynjD"
                     /locus_tag="ACHY3M_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4316320.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26242..27549
                     /gene="ynjE"
                     /locus_tag="ACHY3M_001380"
     CDS             26242..27549
                     /gene="ynjE"
                     /locus_tag="ACHY3M_001380"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFS4316321.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQQVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27558..28178)
                     /gene="ynjF"
                     /locus_tag="ACHY3M_001381"
     CDS             complement(27558..28178)
                     /gene="ynjF"
                     /locus_tag="ACHY3M_001381"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4316322.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28265..28672
                     /gene="nudG"
                     /locus_tag="ACHY3M_001382"
     CDS             28265..28672
                     /gene="nudG"
                     /locus_tag="ACHY3M_001382"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFS4316323.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28638..28910)
                     /gene="ynjH"
                     /locus_tag="ACHY3M_001383"
     CDS             complement(28638..28910)
                     /gene="ynjH"
                     /locus_tag="ACHY3M_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFS4316324.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29146..30489
                     /gene="gdhA"
                     /locus_tag="ACHY3M_001384"
     CDS             29146..30489
                     /gene="gdhA"
                     /locus_tag="ACHY3M_001384"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4316325.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30606..31646)
                     /gene="ynjI"
                     /locus_tag="ACHY3M_001385"
     CDS             complement(30606..31646)
                     /gene="ynjI"
                     /locus_tag="ACHY3M_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4316326.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANIFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31774..33735)
                     /gene="topB"
                     /locus_tag="ACHY3M_001386"
     CDS             complement(31774..33735)
                     /gene="topB"
                     /locus_tag="ACHY3M_001386"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4316327.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33740..34783)
                     /gene="selD"
                     /locus_tag="ACHY3M_001387"
     CDS             complement(33740..34783)
                     /gene="selD"
                     /locus_tag="ACHY3M_001387"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFS4316328.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEIPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(34900..35451)
                     /gene="ydjA"
                     /locus_tag="ACHY3M_001388"
     CDS             complement(34900..35451)
                     /gene="ydjA"
                     /locus_tag="ACHY3M_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFS4316329.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35612..37468
                     /gene="sppA"
                     /locus_tag="ACHY3M_001389"
     CDS             35612..37468
                     /gene="sppA"
                     /locus_tag="ACHY3M_001389"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4316330.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37635..38651
                     /gene="ansA"
                     /locus_tag="ACHY3M_001390"
     CDS             37635..38651
                     /gene="ansA"
                     /locus_tag="ACHY3M_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4316331.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38662..39303
                     /gene="pncA"
                     /locus_tag="ACHY3M_001391"
     CDS             38662..39303
                     /gene="pncA"
                     /locus_tag="ACHY3M_001391"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFS4316332.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHSSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39464..39736)
                     /gene="yeaC"
                     /locus_tag="ACHY3M_001392"
     CDS             complement(39464..39736)
                     /gene="yeaC"
                     /locus_tag="ACHY3M_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFS4316333.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39778..40191)
                     /gene="msrB"
                     /locus_tag="ACHY3M_001393"
     CDS             complement(39778..40191)
                     /gene="msrB"
                     /locus_tag="ACHY3M_001393"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4316334.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGCLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40533..41528
                     /gene="gapA"
                     /locus_tag="ACHY3M_001394"
     CDS             40533..41528
                     /gene="gapA"
                     /locus_tag="ACHY3M_001394"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4316335.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41612..42496
                     /gene="yeaD"
                     /locus_tag="ACHY3M_001395"
     CDS             41612..42496
                     /gene="yeaD"
                     /locus_tag="ACHY3M_001395"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFS4316336.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42547..43401)
                     /gene="yeaE"
                     /locus_tag="ACHY3M_001396"
     CDS             complement(42547..43401)
                     /gene="yeaE"
                     /locus_tag="ACHY3M_001396"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFS4316337.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43491..44237)
                     /gene="mipA"
                     /locus_tag="ACHY3M_001397"
     CDS             complement(43491..44237)
                     /gene="mipA"
                     /locus_tag="ACHY3M_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFS4316338.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44673..46607
                     /gene="yeaG"
                     /locus_tag="ACHY3M_001398"
     CDS             44673..46607
                     /gene="yeaG"
                     /locus_tag="ACHY3M_001398"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFS4316339.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46720..48003
                     /gene="yeaH"
                     /locus_tag="ACHY3M_001399"
     CDS             46720..48003
                     /gene="yeaH"
                     /locus_tag="ACHY3M_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFS4316340.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            48150..49625
                     /gene="cdgI"
                     /locus_tag="ACHY3M_001400"
     CDS             48150..49625
                     /gene="cdgI"
                     /locus_tag="ACHY3M_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFS4316341.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            49806..51296
                     /gene="dgcJ"
                     /locus_tag="ACHY3M_001401"
     CDS             49806..51296
                     /gene="dgcJ"
                     /locus_tag="ACHY3M_001401"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFS4316342.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILAALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            51339..51842
                     /gene="yeaK"
                     /locus_tag="ACHY3M_001402"
     CDS             51339..51842
                     /gene="yeaK"
                     /locus_tag="ACHY3M_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFS4316343.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(51843..51947)
                     /gene="yoaI"
                     /locus_tag="ACHY3M_001403"
     CDS             complement(51843..51947)
                     /gene="yoaI"
                     /locus_tag="ACHY3M_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFS4316344.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            52117..52563
                     /gene="yeaL"
                     /locus_tag="ACHY3M_001404"
     CDS             52117..52563
                     /gene="yeaL"
                     /locus_tag="ACHY3M_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4316345.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(52520..53341)
                     /gene="nimR"
                     /locus_tag="ACHY3M_001405"
     CDS             complement(52520..53341)
                     /gene="nimR"
                     /locus_tag="ACHY3M_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFS4316346.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            53438..54619
                     /gene="nimT"
                     /locus_tag="ACHY3M_001406"
     CDS             53438..54619
                     /gene="nimT"
                     /locus_tag="ACHY3M_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFS4316347.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            54674..55021
                     /gene="yeaO"
                     /locus_tag="ACHY3M_001407"
     CDS             54674..55021
                     /gene="yeaO"
                     /locus_tag="ACHY3M_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4316348.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(55043..55297)
                     /gene="yoaF"
                     /locus_tag="ACHY3M_001408"
     CDS             complement(55043..55297)
                     /gene="yoaF"
                     /locus_tag="ACHY3M_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFS4316349.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            55480..56505
                     /gene="dgcP"
                     /locus_tag="ACHY3M_001409"
     CDS             55480..56505
                     /gene="dgcP"
                     /locus_tag="ACHY3M_001409"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFS4316350.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            56538..56636
                     /gene="yoaK"
                     /locus_tag="ACHY3M_001410"
     CDS             56538..56636
                     /gene="yoaK"
                     /locus_tag="ACHY3M_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFS4316351.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            56639..56713
                     /gene="yoaJ"
                     /locus_tag="ACHY3M_001411"
     CDS             56639..56713
                     /gene="yoaJ"
                     /locus_tag="ACHY3M_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFS4316352.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(56772..>57014)
                     /gene="yeaQ"
                     /locus_tag="ACHY3M_001412"
     CDS             complement(56772..>57014)
                     /gene="yeaQ"
                     /locus_tag="ACHY3M_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4316353.1"
                     /translation="ILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGGWI
                     STLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
BASE COUNT        14112 a        14709 c        13897 g        14296 t
ORIGIN      
        1 tcatcatgat tagcaaaact taaccatttt aaaataaata aacaattaaa gaaaaaagat
       61 cacttattta tagcaataga tcgtcaaagg cagctttttg ttactggtgg tttgaatgaa
      121 tgtagcaatg aaatacagaa tttcaggtca tgtaactccc ggcaaaaccg ggaggtacgt
      181 aatctttact cagtcacttc cccttcctgg cggatctgat ttgcccaacg ctgggcggat
      241 tcaggcacag taaacgccgg tgagcgcaga aatgactctc ccatcagtac aaacgcaaca
      301 tatttgccac gcagcatcca gacatcacga aacgaatcca tctttatcgc atgttctggc
      361 ggcgcgggtt ccgtgcgtgg gacatagcta ataatctggc ggttttgctg gcggagcggt
      421 ttcttcatta ctggcttcac taaacgcata ttaaaaatca gaaaaactgt agtttagccg
      481 atttagcccc tgtacgtccc actttgcgtg tatttcataa caccgtttcc agaatagtct
      541 ccgaagcggg atctggctga tggtctatag ttagagggtt ttttgaccaa aacagcggcc
      601 ctttcagtaa taaattaagg agacgagttc aatgtcgcaa cataacgaaa agaacccaca
      661 tcagcaccag tcaccactac acgattccag cgaagcgaaa ccggggatgg actcactggc
      721 acctgaggac ggatctcatc gtccagcggc tgaaccaaca ccgccaggtg cacaacctac
      781 cgcccctggg agcctgaaag cccctgatac acgtaacgaa aaacttaatt ctctggaaga
      841 cgtacgcaaa ggcagtgaaa attatgcgct gaccactaat cagggcgtgc gcatcgccga
      901 tgatcaaaac tcactgcgtg ccggtaaccg tggtccaacg ctgctggaag attttattct
      961 gcgcgagaaa atcacccact ttgaccatga gcgcatcccg gaacgtattg ttcatgcacg
     1021 cggatcagca gctcacggtt atttccagcc atataaaagc ttaagcgata tcaccaaagc
     1081 ggatttcctc tcagatccga acaaaatcac cccagtattt gttaggttct ctaccgttca
     1141 gggtggcgct ggctctgccg ataccgtgcg tgatatccgt ggttttgcca ccaagttcta
     1201 tactgaagag ggtatttttg acctcgttgg caataacacg ccaatcttct ttatccagga
     1261 tgcgcataaa ttccccgatt ttgttcatgc ggtaaaacca gaaccgcact gggcaattcc
     1321 acaagggcaa agtgcccacg atactttctg ggattatgtt tctctgcaac ctgaaactct
     1381 gcacaacgtg atgtgggcga tgtcggatcg cggcatcccc cgcagttacc gcaccatgga
     1441 aggcttcggt attcacacct tccgcctgat taatgccgaa gggaaggcaa cgtttgtacg
     1501 tttccactgg aaaccactgg caggtaaagc ctcactcgtt tgggatgaag cacaaaaact
     1561 caccggacgt gacccggact tccaccgccg cgagttgtgg gaagccattg aagcaggcga
     1621 ttttccggaa tacgaactgg gcttccagtt gattcctgaa gaagatgaat tcaagttcga
     1681 cttcgatctt ctcgatccaa ccaaactgat cccggaagaa ctggtgcccg ttcagcgtgt
     1741 cggcaaaatg gtgctcaatc gcaatccgga taacttcttt gccgaaaacg aacaagcagc
     1801 attccatcca ggtcatattg ttcctggtct ggacttcacc aacgatccgc tgttgcaggg
     1861 acgtttgttc tcctataccg atacacaaat cagtcgtctt ggtgggccga atttccatga
     1921 gattccgatt aaccgtccga cctgccctta ccacaacttc cagcgtgatg gcatgcatcg
     1981 tatggggatc gacactaacc cggcgaatta cgaaccgaac tcgatcaacg ataactggcc
     2041 gcgtgaaaca ccgccgggac cgaaacgcgg cggttttgaa tcatatcagg aacgcgtgga
     2101 aggaaataaa gttcgtgagc gcagtccatc gtttggcgaa tattattccc atccgcgtct
     2161 gttctggcta agtcagacgc cattcgagca gcgccatatt gtcgatggtt tcagttttga
     2221 gttaagcaaa gtcgttcgtc cgtatattcg tgagcgcgtt gttgaccagc tggcgcatat
     2281 tgatctcact ctggcccagg cggtggcgaa aaatctcggt atcgaactga ctgacgacca
     2341 gctgaatatc accccacctc cggacgtcaa cggtctgaaa aaggatccat ccttaagttt
     2401 gtacgccatt cctgacggtg atgtgaaagg tcgcgtggta gcgattttac ttaatgatga
     2461 agtgagatcg gcagaccttc tggccattct caaggccctg aaggccaaag gcgttcatgc
     2521 caaactgctc tactcccgaa tgggtgaagt gactgcggat gacggtacgg tgttgcctat
     2581 agccgctacc tttgccggtg caccttcgct gacggtcgat gcggtcattg tcccttgcgg
     2641 caatatcgcg gatatcgctg acaacggcga tgccaactac tacctgatgg aagcctacaa
     2701 acaccttaaa ccgattgcgc tggcgggaga cgcgcgcaag tttaaagcaa caatcaaggt
     2761 cgctgaccag ggtgaagaag ggattgtgga agctgacagc gctgacggta gttttatgga
     2821 tgaactgcta acgctgatgg cagcacaccg cgtgtggtca cgcattccta agattgacaa
     2881 aattcctgcc tgatgggagg cggcgcaatt gcgccgcctc aaatgattta catagtgcgc
     2941 tttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaagtcg tataaattca
     3001 atgtattgca gtttattggt agatcctgat aagcgaagcg catcaggcag ttttgcgttt
     3061 gtcagcagtc tcaagcggcg cagttacgcc gcctttgtag gaattaatcg ccggatgcaa
     3121 ggttcacgcc gcatctggca aacatcctca cttacacatc ccgataactc cccaaccgat
     3181 aaccacgctg agcgatagca cctttcaacg acgctgatgt caacacatcc agctccgtta
     3241 agcgtgggaa acagtaagca ctctgacgga tagtattatc gataaacgcc ggatgacaca
     3301 tcacctccag cgaacgatca ccccgatggc ttgcgtcatc cagcacttgc aggaacagtg
     3361 cctcgctaat ctcttcacca taaaacgcgc tactgaatcc ctgagaactg cgcagattag
     3421 ccggtaaatc accagcgtta gatagcggct gacggtcgat gcgcaacgca atcccctctt
     3481 cagccgcaaa cctggcaaca atcgggaaaa tctgcgggaa catatgcaca tgatgatggc
     3541 tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac tgactggcaa
     3601 gctcctgagt aatttcttcc agcggtaaag catcttcttc tgccaactgc cagatccatt
     3661 ttcccagcac accatcgcgg gttaaccccg gcatagctgt caggggcttg cccatagtaa
     3721 ggacaaagtg catccctatg gccagactcg gttcatcacg actcaactgc accgcatggt
     3781 caatagcctg cccattcacc agcgccgtcg ttgatgtgac aatcccattg cgacaggcct
     3841 cgataatgcc gtagttctga cctttgctta agccaaaatc atcggcatta acaatcagta
     3901 agcgttccat aatcagcctc ggttaatgtg cttttttaag ctctgcgatg cagtcggcaa
     3961 aatttggcag ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc tcagcatcgc
     4021 gatcggaatg caccaacgga ctaaggttta gcgccagtaa cacatcgtta aattctccgc
     4081 taagtgcggc gttgctggca gcaatctcga agcctttaat ggtgtgaatc agccccatca
     4141 ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc cccagcttac
     4201 aggtcatttc taccgcccag tctgccggaa tattatcaat atgcccatga tgcgggatat
     4261 taacgtaatg ttcagcttgc ttgtcgttgt agatagcgtt gatcacttcg cacgctgcat
     4321 cagagtaata agccccaccg cgctgttcca gttctttcgg cttaactttc aactccggat
     4381 ttttatacag ctcaaaaagt tgtttctcga ctttctgtac tacctgtgct cgtgcgccgc
     4441 ctttgtagta ttcgcccatt tcaatagcca gcatctcttt ctgcttgaag taatacagca
     4501 gataagaaca tggcagcaga tttaacgaac gaattaagcc ctcactaaat ggcagatcga
     4561 aaatattttt tacagaggac gcttttaact gccctgacgc cacaccatca agcaattcgg
     4621 caaagcgcga cttgccattt accagcacat ccttaatgaa caccatatgg ttgaggccga
     4681 acagatcgat agataaatca tcgctgtctt tcagcatcag aacatcgcga ataaacatct
     4741 tcatgccgat cggaatatta cacacgccga taaagcgttt aaatccggta tgacgataaa
     4801 cggcttcagt gaccattccc gccgggttag tgaagttaat cacccatgca ttcggacaaa
     4861 gttcttcgac atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga cctttaaaca
     4921 gaccgcccgc gccgttggtt tcctgaccaa gataaccatg acttaatgga atacgttcat
     4981 ccagttcgcg cgccggtaat tggcctacgc gcagttgggt agtaacgaaa tcagcatctt
     5041 tcaatgcttc gcggcgatcc agcgttttat aaagcttcat cgggacgcca gcgttatcaa
     5101 tcatccgttg gcagagttca aaaatgatat ccagtttctc tttaccatct tcgacatcca
     5161 ccagccataa ttcgctgacc ggcaattcgt gataacgctt aataaatcct tccagtaact
     5221 ccggggtata gctgctcccg ccaccaatag tgacgacttt taatttctgg ctcataattt
     5281 ctcccttcag tacagaatac tgatatctgg catatctgcc cccccggaca taaataatcc
     5341 agcaacagga cagatatgtg aattgtcagg tataacgact tactgattaa attcagtcaa
     5401 tttcttacga tagctcttag gcgtaaagga tgttaatttc ttaaacgttt taataaacaa
     5461 actcgggcta ctataaccgg cctcaaacgc aatatccgtc acggaatagt tggtcatttc
     5521 cagttgtttt ttggcaaaat taatacggat ctcattaata atctgcattg gtgttttgcc
     5581 ataatatcgt tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca acgtcaccat
     5641 attctccagc gccgattcac taaactgctc tttatcatgc atcttttcta ccgtactttt
     5701 cagccactgc ggtacatcat caatcacctg ttcttcgcgg taatgacgta aacggttaat
     5761 gacataaaaa gtaaccatct caacaaactc ttccagccct gtttcgcgga aattcaatga
     5821 agaaatcact gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc
     5881 gacaaagcaa taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc
     5941 aacgttcaat atacgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg
     6001 aataaaaaca aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctggaaata
     6061 acgcccggtt aataccagag taaattcata atagtcgtgc tgatgcagcc cgctgatact
     6121 ctcagttttg ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg
     6181 ggcagtggca atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca
     6241 gtttttcatg aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta
     6301 aatgatcctg agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc
     6361 cttcaatcag tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca
     6421 tcatggcttt tgctgcagca aaatcgccct gcttcgcctg tttcagtgcc gcatacgcca
     6481 ggctgcgcgc ttgtccggag ttgatgataa gccccatcac cacttcttcc agctcttcag
     6541 cttctgtttg cgtatcagga atattatcga gatccatcat acatcgttcc tctctcttct
     6601 taccggcaag attacccgta ccggcatcga ttaaaatttc agggcgttag cgatatcttc
     6661 ttcgctctct tctttatcaa tcgcattttg tgctttgtta gccaccacaa caaagggcag
     6721 ataaattaag gttgcgatgc caaggttgaa gagtgcgacc agcaatgcgg cgacgctacc
     6781 gttggtgtta aagaaggctc ccagaccggt tggcatggtc cacggtgcaa tattggtcac
     6841 cggaggaatg atgcccatgt agtacgctgc gagggtgatt gctgccagaa tcggttgtac
     6901 cagtacaaac gggataaaca tcaccgggtt catgataatt ggcagaccaa acagaatcgg
     6961 ttcgttaatc tggaagatgc cggacggcag cgccagtttt gccacctgac gataatcagc
     7021 acggcgagag gcgataaaga tagccaggat caggcctaaa gtcgcaccac tgcccccaag
     7081 gaaaataaag gagtccagca ccggcttggc ccagatatgg aaggtcttac cggctgccag
     7141 cgccgcttcg acggaaccat attgctgata ggtcgcgata ttttccagcg cccacggcgt
     7201 cataatgccg ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga agaaccagag
     7261 cagtggaaca aagatcacat aggcccagcc caccacgcta cccaacgatg ccagtggggt
     7321 tgagatggta tccataatga tctgatggaa gttggtgccc caggtattca acgcccaggc
     7381 aataatcccc atcacggaaa gaataataaa gccgggaatt aatgccgaga aggaacgcga
     7441 tactgaagca ggtacgctgt cgggcagttt aatgacccaa ttgcggcgga caataaaggt
     7501 aaacatttct gccaccacca ggccaataat aatcccggag atgatattcg ccccacctaa
     7561 ccagtttgcg ccaaccgcat aggcctcacc gacactatat ggggtgacgg tcataaatgc
     7621 tgcaacggat aacaaccccg ccgccagcgc atcgacttta cgctcttctg ccagcgccat
     7681 gccaataaag aacggtgcca ttaaagacat tatccctaat gttccgttat atacgttgcc
     7741 gccaatacct ttcaggccat taagtgtttc aatggttgag gcgtcgaggc gaatacctaa
     7801 ggaataaaaa aacgacccct ccccaaagct tagaaaaacg ttgttaatta atacaaacat
     7861 ggcccccgca agggttaacg gcattaagcg aataaagcca tttttgattg cattaacgtg
     7921 tggctgcttt cctattttaa ctgcaaaagg gaggagtacc ttttcaagcg atgcaataac
     7981 attactcata gaaaaatacc cttaaaaacc gcaatttaaa tattgcggta ttgatttatg
     8041 aaataactct ttgacgggaa aatttaaaat aaattaattt gctgcggctt ttttaatcgc
     8101 tgcaaccgca gccttaagca cgcctaaacc atcgactttg ccataaagca gcgagtcaat
     8161 tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat aagcaatctg
     8221 cggccctaat aacacgacat cggcattctg acctttttca ccagccagtg tttccggaaa
     8281 tgcttcaata atgaccggaa cttcatattt ttctgcctgt gcgcgcattt ttgataccag
     8341 taaagaggta gacatgcccg cagaacaaaa cagataaatg tgtttctttt ccataaaact
     8401 gccctcatcg acgattatct gtcagccaga cactccgcaa gccttaacct gcttccatgc
     8461 tctgggtaac ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt atacggtata
     8521 gacctgagca atagtatttc tttgacctta taaattgttt ctccttgacc tgtggttatg
     8581 accctcttca catttcgggc aaataattcg cgaagataat taagaactca ggccaaaaaa
     8641 aaccggcgca atggccggtt tccgttgtta ctcaagcttt cagacgaatt gattacttcg
     8701 cagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg atgacatgtc
     8761 cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga cccaggatgt
     8821 aggtctggca agtaccgcga gcatggatca tgctcacttc agacgaaggt ttacccgcaa
     8881 tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa
     8941 actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat accgccgctg
     9001 cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat cagcgtgtta
     9061 gataagcctg gaatacattg ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc
     9121 agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt
     9181 agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt caatgacatt
     9241 gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgctg aagaggaaat
     9301 tcgtcgtagt gtcgattttc tgaaaagcta cctgcaaact tatccgttca ttaaatcact
     9361 ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt gccagatggc
     9421 gattaatgag ctgcgccagg aaactggcaa cgaatcactg caatttattg ccgtacgcct
     9481 gccctatggt gttcaggctg acgaacaaga ttgccaggat gccattgcct ttattcaacc
     9541 ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg gctagcgagc aggcattgcg
     9601 ggaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat
     9661 gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc
     9721 agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacgg atattaatcc
     9781 gctgtatcgt ctcaacaaac gccagggtaa acagttgctg acggcattag gttgcccgga
     9841 acacctttat aagaaagccc caacggccga tctggaagat gatcgccctt ctctgccaga
     9901 tgaagtggca ctcggcgtga cctatgacaa tatcgacgac tatctggaag ggaaaaacgt
     9961 acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac ataaacgccg
    10021 tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac ataatggttt
    10081 gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg aacgccttat tcgacctaca
    10141 aaactatgca aattcaatat attgcaggag tcgaataggc ctgacaggcg tagcacgtca
    10201 gacggtgtaa cctttgtcat cgacccgcct cttttttaat cgcttcccgc ctgttacact
    10261 ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct ccgcggctcg
    10321 aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc cttaatgact
    10381 tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg ccgctctata
    10441 tcggcaaaag cgttaatatc cgcagtcggg tgctttctca tttacgcacc ccggatgaag
    10501 ccgccatgct acggcaatcc cggcgaatta gctggatctg caccgcaggc gaaatcggcg
    10561 ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat aaacggttgc
    10621 gccgcaatcg ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat gtggtgtatg
    10681 ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc aataggcgcg
    10741 cagctttgca agcattgcag agcatcgctg atgaacaaaa actttgttat ggcctgctgg
    10801 gactggaacc gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg
    10861 gagcatgctg cggtaaagag agccatgagg aacatgcgct acgcttgcgc cagtctctgg
    10921 agcgtttgcg ggtggtgtgt tggccttggc aaggggcggt ggcgctgaaa gaacagcacc
    10981 cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg gttaattcgc
    11041 tggaagacgc gacaacgtta attcggacac ccgccgggtt tgatcacgac ggttataaaa
    11101 ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat ccggcgaatg
    11161 accagcgagc cagttgattt cactgaacaa cagtttacct tgcggttgta aaagccgcag
    11221 ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcagtt tgtcgcccag
    11281 ttgccacgcc ccgttaatca caaacactac cccgccgcgt gagccaaagg tagtaaacgt
    11341 acgttcggca attctgactt tcgccttaca gacatccagg cgcgtcataa tattgaaatc
    11401 catcgacatc tgccctgccg tcagtttcgc ttttaccacc tggtccgccg caaaggcaaa
    11461 aggctgtaac ggttttaagg tatggttaaa gtggtctgcg ctttcaagga acatctcgcc
    11521 gccttccagc aacgtcacta tcctttccat accgggaaac aaagaaaact ccccattcgc
    11581 cgcgatggat gcaatgctgg cacgccagta aaaatcacgt tttgccggtg ggaaagtgca
    11641 aatttcgcgc gtttcaccgg cagcatttcg ccacagattc accgacattt tacgcatatc
    11701 aaagtattcc atactcgctc ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg
    11761 gatggttatc ggcaactcgg gtgatttacg ttagtggtga tcaggctttt tttacaactg
    11821 tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac
    11881 aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca ggcattttac
    11941 cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct
    12001 gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc agcatgttag
    12061 ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat
    12121 cgctggcaac gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt
    12181 cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt
    12241 ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcata atcggcttcg
    12301 cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag
    12361 ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca
    12421 gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact
    12481 ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc
    12541 caattatgga gaaaaaaaga aaatttgatg gagagtgatg agagaatatt acaacacgat
    12601 gattttgcag agattatgaa gaactatacc ggatgactgg tgataaataa agcaaataac
    12661 caggattaat ctgtattaat ttataagaaa gcaacttaat acccgcagag tgatttctgc
    12721 gggtaagtat tagcttattt tttcgagcat taatcccgcg cgtaatccca acgctaccaa
    12781 cggattaggg aataacacat actctacatc atgggttacg gtaaaacgtt cctctccgtc
    12841 ctgcgccagc aatgttcctt tctcaaacgg cataaaattc agcgtgtcac ttgccatatg
    12901 catttcgaag gacggcgagt ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg
    12961 tgttctcacg ataccgacac tctcaccaga tagcagcgca gcaattgcgc tggcagttac
    13021 tgcaaactgg cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt ccagcgtaca
    13081 ggccagcgcg ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag gttcctgatg
    13141 gaacaccagc gcctccagcc ccgccgcacc cagccacgtc agaaatttct cgtcccaggg
    13201 aatgtcgcgt tgcggtaata caccgaactg cggatgcaag gagccacgaa ttgcggtatg
    13261 tagatcaagg tgccagcgca cagattcttt gccctggtca taaaaatctt ccaggcactg
    13321 ttccagttcg cgcgcccgac aggtttctcc gctttcagca aatagctgcc aacgaccgcc
    13381 aaacattcga ttcatatcgc tatggcaata acgtttccct tgcttcagcg caggaggatt
    13441 cccgaggatc accagcaacc gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag
    13501 caacgcgtcc agcatctcca caggtgccgt ctcattaccg tgtattcccg ctgaaatcac
    13561 cagtgcgcct tgcggtggcg ttaatggcgt cagttccagc acaccatcgc ccagccagcg
    13621 ccagcgaacg ccgttgattt ctcgctcggt gataaccggt tttttacccg ttaaggtcag
    13681 agcaagaaaa ttatccattg ccccctccct cgcgctggaa cggataaacc gaaccgagat
    13741 tcagtaattg gctcaataca tccagtgctt cccgcccttc gcgcagcaat tgcgggtcgg
    13801 ccagatcggc agcagtaagg cgatcgcggt agtaacgatc cacccagtca ttgagcgcat
    13861 taaacagcgt atcgttcatc atcaccgccg gattcaccgc ccggcgttct tcttctgtca
    13921 ataccacccg caaccgcagg cacgccgggc cgccgccatt cgccatgctt tcacgtaaat
    13981 caaagacttt tagttctcta atcgggttgt cagcggcaag gagttcattg agataacccc
    14041 atactccggc gtgttcccga cactcctgag gcagcaccaa catcatggaa ccatcatcgc
    14101 ggctcaacag ttggctgtta aacagatagg tagacaccgc atccgacacg gaaacctgag
    14161 ttgccggaac ttctatcgcc ataaaaccgt tcacccgcgc acgcaggttt gccagtaact
    14221 gtgactggcg agcgaacgcc tgttggtggc aaaacagcac ctggcggtta ctcacggcaa
    14281 tcacgtcatt atgaaaaacg ccctggtcga taacgtccgg gttttgctgg gcgaaaatca
    14341 cctgttgggg attcacctga ttcagccttg ccaccgcctc gctggcttcg cgagtctgtc
    14401 gcgccggata acgggaaggc cgggtatcat tgccttcttc tcgcccgtag acaaaaagtt
    14461 gcatacccgg ctcaccgtaa tgaccgccga gacgattgtg gtttgccgcc ccctcatcac
    14521 cgagcaacgc tacctgcggc aacgccgaat gaacgctaaa tttctcttcg tcgttaaaaa
    14581 tcgcttttaa aagtgattcg gtgacgtgcg cttccagcga gcggtgaaat ttattgttca
    14641 ggttggcaac ggtgagatgc actttgccat ccagcgtatc ggcagatggc gcgatcgttg
    14701 ccgcattggc tacccacatt ggcgaagcgg aactgacgct ggaaagccag tgcggtgcct
    14761 ggcgtgcaac tttttccagt acctgctcat cgctaccact gaatcccaac tgacgcagca
    14821 ccggaataaa cggacgctcg tgcggcggga tcacggcctg ggggaatccc gcatcggcaa
    14881 gggctttcat tttcagtaag ccctgttttg ccgcctgtcg cgggttagac acctgaaaac
    14941 ggtgacgggt agaggcttca ttaccaaacg acaggccagc gtaatgatgc gtcagcccta
    15001 ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc acctcatcgg aaaaatccag
    15061 cccggggtta agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg ccataggcca
    15121 tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg ttaccggatg caccaatgcc
    15181 gccgaatggc gcggtactgg cagcaccagt aagcggtttg ttccagttaa caatccccgc
    15241 tcgcgcctcc agcaacagtt gatcgaattt ttcccgctcg ggggaaacca gaccgcaaga
    15301 gagtccgaag cgagtgttat tcgccattag aatcgcttcc tcgaaagaat cataacgcca
    15361 gacgcgcaat aacggtccga acacctcttc atctggtacg ccagcaacgc ctgtcatttc
    15421 aatgatcccc ggcgtcagca acgatgtctc tgattgtaat aagcgcggcg caagcagggt
    15481 tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc acctgctgtg cggcctgttc
    15541 agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg gcgttaatcg
    15601 ctggctgacg gcaaccagac gagcaagaaa cgcatcgccc tgcgctccgc ttttgagcaa
    15661 taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca aacgccgact gaatggtcag
    15721 atggacagcc gcgtcgatat ccgccacctc atcgataatt agcggattat taccgcccat
    15781 ctcaagggcg agaattttct ccggctgacc ggagagctgg cgatgcagct ggtagccggt
    15841 attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca gcgcctgacc
    15901 cgtttcacgc ccgccctgca ccaggttcag tacgcccggc ggcaagccag cctgctgcca
    15961 taaacgcatt accgcttcgc cactccacgg tgtcagttcg ctgggtttaa agataatggt
    16021 gttacctgcc agcaatgccg gaacgatatg tccgttcggc aaatgaccag ggaaattata
    16081 cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc aggctcgccg caccgtccgg
    16141 catttcacta cgctgctcgc cggtacgaac gtgatacgcc ttaattgata tcgcgatttt
    16201 attgatcatc gccgtcactt cggttgccgc ttcccagcgc ggcttacccg tttctctggc
    16261 aataatcgcg gttaattcgc ctttattcct ttccagcaat ccggcaaagc gttcgacaac
    16321 gacctgacgt tcagccaatg agagccgcgc ccagcgcgga aacgccgcac gggctgcccg
    16381 acaagcctgt tcgacctgag cggcatcggc atcattgcct tgccataaca cctcgcccga
    16441 taccggatta cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca
    16501 taaagtcatg ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg atcccctgcg
    16561 tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg
    16621 gtacgcgcca gcaccacgcg gaaatggtga taattttcat tggcgaccag gcaggccgga
    16681 aaatcgccct gcgcaggctg cccttctgcc acttccacca gccgactttt acgaatggcg
    16741 cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg
    16801 cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc
    16861 tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg atgtttcggc
    16921 atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa
    16981 tccatcgaaa agaagcgttt accgaggctt tgccagaacg gtgaatagcc gtgttcgtca
    17041 atcaccccgc gcatttcagc aaccactttg tcattaaact tgtcgcgaaa agccgccata
    17101 aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg
    17161 agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa cagcgtcggc
    17221 aatgcgttat agacattcag ctcttttgag gcgtgaacca acgtgccgac gcgatagttg
    17281 taccagggat cgttcagccc aaccgccacc tcaatggcac aaatccccgc cacggtgcct
    17341 gtctcgctat cttccagcac gaacacatag ccctgctcac ttttgggcag ttcgccttgc
    17401 caggttttga ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc gggaagcgac
    17461 gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc tgatcgctca
    17521 acgggacgga tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa
    17581 agcgatccag tccggtcgtc acctcttctt cgctgacatt gagcgcaggc gcaaaacgca
    17641 ccacgttgcc acccgcaatc agtaccatca cgcctgcttt cgccgcttcc tgagagatct
    17701 gtttcgcttg cccggcgtaa tcggcattca gtacacagcc aatcagcaaa cctaagccgc
    17761 gaacttcact gaacaaacca tagcggtgat taatagtatt aagacgctca acaaaccagt
    17821 cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc agcactttgc
    17881 ctgccaccgc cgaggccagc ggattaccgc cataggtggt gccatgagtg ccaacggtca
    17941 tcacgcgggc gcactcttcg gttgccaaca acgcaccgac cgggaaaccg ccgcccagcg
    18001 ctttggcggt agttaacaga tcaggcgtca cgccgtagtg catataggca tacagttccc
    18061 cggtgcgccc gacgccggtt tgtacttcat caaaaatcaa cagcgcattg tggcggttac
    18121 acaattcacg cagaccttgt aaaaacgcgt tgctggctgg caccacaccg ccttccccct
    18181 ggatgggttc gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg gcagagttaa
    18241 tatcgttata tgcagcatga cgaatatccg ccggcagtgg cgcaaaatcc tgtgaatagg
    18301 ctggctgccc acccgcactg acagtaaaca gcgtgcgacc atgaaacgca tttttgaacg
    18361 ccacgatgcc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta
    18421 gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg
    18481 cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat
    18541 gccagaactt actcgcctgt tcgttcagcg cttcacgcag ttccggatgc gcatggccca
    18601 gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttcccctgc tgatcccaca
    18661 agcgcgaacc ttcgccacgt accggtataa agggtgccgg agcgtaaaca ggtatcatcc
    18721 attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca gtaaataatt
    18781 cgttatttat atgttaacaa taagtaatgt ttgcgctgta aatgtagatt gcagggttcg
    18841 tgccagccag tgataaaagt gcataaacgg cggaggctaa ctggaaatca aggagttata
    18901 accaaaccat atgcatttaa agtgcatata aagtgaatac gtttgcgatg tgggtgaata
    18961 aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa ttgtgatcat
    19021 tgacgaaatt tactggaaat tactgcgcca ttctgacgca gcgcgcacca aaagcgggca
    19081 ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta acaacaggcg
    19141 gtaagcaacg cgaaattctg ctaccatcca cgcactcttt atctgaataa atggcagcga
    19201 ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac cagcttgaag
    19261 ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa gttcatgacg
    19321 atatgtttcc gctcgaagag gtggcgaagc tcggctacaa cgtgttttat cacgggcaga
    19381 aaggccatta tggcgtggcg ctgctgacca aagagacgcc gattgccgtg cgtcgcggct
    19441 ttcccggtga cgacgaagag gcgcagcggc ggattattat ggcggaaatc ccctcactgc
    19501 tgggtaatgt caccgtgatc aacggttact tcccgcaggg tgaaagccgc gaccatccga
    19561 taaaattccc ggcaaaagcg cagttttatc agaatctgca aaactacctg gaaaccgaac
    19621 tcaaacgtga taatccggta ctgattatgg gcgatatgaa tatcagccct acagatctgg
    19681 atatcggcat tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa tgctctttcc
    19741 tgccggaaga gcgcgaatgg atggacaggc tgatgagctg ggggttggtc gataccttcc
    19801 gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt
    19861 ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat
    19921 gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg
    19981 cccccgtctg ggcgaccttc cgccgctaat ttagcagctc tcctggctca aactgggtca
    20041 ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc
    20101 tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat caagtttgat
    20161 ataggaaact ccacgatgaa cgctgagcgt aactttctgt ttgcctgtct tatttttgcg
    20221 ctggtcattt acgctatcca cgctttcggt ttattcgatc tgctcaccga tttaccccac
    20281 ttacagacac tcattcgcca gagcggattg ttcggttata gcctctatat tctgttattc
    20341 atcattgcca ccctctttct gttaccagga agcatactgg tgatcgctgg aggaatagtt
    20401 tttggcccgc tcttagggac actactctca ttaattgccg caacgctggc ctcctcgtgc
    20461 tcattcctgc tggcgcgctg gctgggacgt gatttactgc tgaaatacgt gggccatagc
    20521 cataccttcc aggccattga aaaaggcatt gcgcgtaacg gtattgattt tcttattctg
    20581 acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg attaaccacc
    20641 atcgccttct ggccttatac ccttatttcg gcactcacga ccctgcccgg tattgttatt
    20701 tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgcgctt tattttacaa
    20761 ctctgtctgg cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact ctacgcccga
    20821 cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac ttactcaccc aaaaaatgaa
    20881 ggatagaacg atgtcgcaac attattcagt gtcatggaaa aaaggactgg ctgcactctg
    20941 cttactggct gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg cggcaaaagt
    21001 ggaatacgac ggactttcga acagccaacc gctgcgcgtc gatgccaata accatacggt
    21061 aaccatgctg gtgcaaatta atggtcgttt cctcaccgac gacactcgtc acggtattgt
    21121 gtttaaagat ggctccaacg gacataaatc gctgtttatg gcttatgcga ccccgaaagc
    21181 attttatgaa gccctgaaag aggcaggtgg tacgccgggc gaaaacatga cgatggataa
    21241 taaagaaacg actcatgtca caggcagcaa actggatatt tcggtcaact ggcaaggggc
    21301 ggcaaaagcg tattccttcg atgaagtgat tgttgacagt aatggcaaga aactggacat
    21361 gcgctttggc ggtaatttaa cggcagcaga agagaagaaa acgggttgcc tggtgtgtct
    21421 ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac acttatggtg cggtggaaaa
    21481 acgcggcgaa gtgaaattca aaggcaatgc ctcagttctc ccggcggata acacgctggc
    21541 aacggttacc tttaaaatca ccgaataaag ccaggataaa ggatgatgaa gatacaatcg
    21601 cgtaaaatct ggtactaccg tataaccctc atcatcctgt tgttcgctat gctgctcgca
    21661 tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca gcgttgcggc gtttgccgcc
    21721 gtggaccaac agggtataga acgctttatt cagtcttacg gcgcactggc ggcagttgtc
    21781 tcgttcttgt tgatgatttt acaggccatt gctgcaccgc tacctgcgtt tttgatcacc
    21841 tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct tgctgtcgtg gaccagttcg
    21901 atggccggcg cggcgctgtg cttttttatc gccagagtga tgggccgcga agtggtggaa
    21961 aaattaaccg gcaaaaccgt gcttgacagt atggacggct ttttcactcg ctacggcaaa
    22021 cacaccatcc tggtctgtcg gttattgcct tttgtccctt tcgatccaat cagctatgct
    22081 gccggtctgg cttcaatacg ttttcgctcg ttttttatcg ccaccgggct tggtcaatta
    22141 ccggcgacta ttgtttattc ctgggcgggc agcatgttaa caggcggtac tttctggttt
    22201 gtcaccggac tgtttattct gtttgccctg accgtggtga tttttatggc gaagaaaata
    22261 tggcttgagc gccagaagag gaatgcctga tgggtttacc gccgcttagc aaaatttctt
    22321 tcattttacg tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg ctaagcccga
    22381 ttcgctggtg ggggcggatc ccgtttatct tttatttggt gtcaatgttt gttgggtggc
    22441 tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc gcttgtcagc gcgcgcattg
    22501 cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc gccgagcgta
    22561 ccggcagcac cgataaactg ctggcagtgg ctgactggcg gcaaagcccg ctcttcagcg
    22621 atgaagaacg gctggcgctg gagtacgccg aagccgcaag cgtaacgccg ccaacggtcg
    22681 atgatgccct gcgcacccga ctggctgcgc attttgacgc tcaggcgctc accgaactga
    22741 cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg gacattcccg
    22801 ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg agagaagatg cgccattgtg
    22861 ggtggttgct gggattgtta tcgctgtttt ctctggcaac acatgccagt gactggcagg
    22921 aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc ggcgataccg
    22981 caattaaccg ctatctcgac tgggttagcg gcgagatgaa aacccattac gctataaacc
    23041 tgaagattgt tcgtctggcg gatgccgcag acgcggtgaa gcgcattcag accgaagctg
    23101 ctgccggacg taaaacgggc ggctcggtgg atctgctctg ggtgaacggc gaaaacttcc
    23161 gcaccttaaa agaggccaat ttattacaaa cgggctgggc ggagactctg cccaaccggc
    23221 gctatgtcga cacacagctg ccggtgcggg aagatttttc agtgcctaca gaaggggctg
    23281 aatcgccctg gggcggcgca caactgacgt ttatcgcccg ccgcgatgtt acgccacagc
    23341 caccacaaac gccgcaagcc ttactggagt ttgctaaagc caatcccggc acggttacct
    23401 atccgcgccc accggacttt accggcacgg cgtttcttga acagttgctg attatgctga
    23461 cgcccgatcc cgccgcgtta aaagttgccc cggataaagc cactttcgcc cgcgtcactg
    23521 cgcccttgtg gcaatatctt gacgcgctac atccctattt atggcgcaaa ggaaaggatt
    23581 tcccgccttc gcccgcgcgg atggatgctc tgctgaaagc cggcacattg cgcctgtcgc
    23641 tgacctttaa ccctgctcat gcccagcaaa aaatcgccag cggcgatttg cctgcaagca
    23701 gttacagttt tggttttaac caggggatga ttggcaacgt gcatttcgtc accattcctg
    23761 ctaacgcaaa tgccagtgct gcggcgaagg tggttgccaa cttcctgctc tcacccaatg
    23821 cgcaactgcg taaagcggat cccgctgtct ggggcgatcc ttctgttctc gatccgcaaa
    23881 aactgcctga tgggcagcgc gaaacattgc aatcaagaat gccgcaagat ctgccgccgg
    23941 tactggctga accgcacgca ggttgggtaa atgcgctgga acaagaatgg ctacaccgtt
    24001 acggtacgca ttaatctttt tgctgtgggc gatggtggcg gtgatttatg caccactgat
    24061 cccggcagct ctcacgctga tatcgcctgc tttgtcgttg acacactggc aggcgttatt
    24121 tgccgatcca cagttaccgc aggcattact ggcaacgctg gtgtcgacaa ccatcgcggc
    24181 ggtcggggcg ttgttgattg ccctgctggt gattgtggcg ctgtggcctt ggccgaaatg
    24241 gcagcgtatg tgtgcccgtc tgccgtggct gctcgccatt ccccatgtgg cttttgccac
    24301 cagcgccctt ctgctctttg ctgacggagg gctgctttat gactatttcc cgtatttcac
    24361 tccgctaatg gacaaattgg gtattgggct gggcctcacc cttgcagtga aagaaagcgc
    24421 atttctgctg tggatcttag cggcagtatt gagcgaaaaa cggctgttgc agcaggtcat
    24481 tgtgctggat tcgctgggct acagccgctg gcaatgcctg aactggctgc tcttgccctc
    24541 cgtcgcacct gcactggcaa tggcgatgtt ggcgattgtt gcctggtcgc tttcggtcgt
    24601 ggatgtggcg attattcttg ggccaggtaa tccaccgacg ctggcagtaa ttagctggca
    24661 gtggttaact cagggcgacg cggatcaaca aacgaaaggc gcactcgcca gtctgctgct
    24721 gatgctgtta ctcgccgcct acgttttgct gagctatctg ctatggcgca gctggcggcg
    24781 cactattccc cgcgtagatg gcattcgcaa gcctgccaca cctttattac caggcactac
    24841 gctggcgagt tttttaccct taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc
    24901 ggatcagtcg acgatcaata gtgaagcact catcaacagc ctgacaatgg ggctggtggc
    24961 gacattcatc gctttgctcc tgttactgct gtggctggaa tgggggccac agcgtcgcca
    25021 gttgtggctc tggttgccca ctctattgcc cgctctgcca ctggtggcgg gccagtacac
    25081 gctggcgcta tggctgaacc tggatggaag ctggacggcg gtggtctggg ggcatctgct
    25141 gtgggtaatg ccgtggatgc tgtttatcct gcaacccgcc tggcagcgca ttgattcacg
    25201 gttaattttg attgcgcaaa cactgagctg gtcgcgggcc aaaatcttct tttacgtgaa
    25261 atgtccgctt atgctacgcc ctacgctgat tgccttcgcg gtgggatttt cagtcagtat
    25321 tgcgcagtat atgccgacgc tgtggctggg cgcggggcgt tttccgacgc tcaccactga
    25381 ggcggtggca ttaagcagcg gcggcagcaa tggtattttc gccgcccagg ctttatggca
    25441 actgctatta ccgcttatta tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta
    25501 tgtcagacaa ggactccgct aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa
    25561 agccgcttgc tgacaaacgt taactttacg gtggataaag gtgacattgt cacgttaatg
    25621 gggccgtctg gctgtggaaa atccactctg ttttcatgga tgattggtgc actggctgga
    25681 cagttttctt gtacaggtga gctatggctc aatgagcaac ggattgacat gctacccacc
    25741 gcacagcgtc agattggcat tcttttccag gatgcactgt tatttgacca gttcagcgtc
    25801 gggcaaaatt tactgctggc gctaccgtcg acgctcaagg ggactgccag gcgcaatgcc
    25861 gtgaaagacg cgcttgatcg agcgggactg gctgaaactt atcaccagga ccctgcgaca
    25921 ttatcaggcg gtcagcgagc gcgggtggcc ctgctgcgcg cccttctcgc tcagccaaaa
    25981 gcgttactcc tggatgagcc attcagccgt cttgatgtgg ctctgcgcga taattttcgc
    26041 cagtgggtgt tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt aacgcacgat
    26101 ctccaggatg ttcctgctga tagttctgtt ctggatatgg agcagtggtc agaaaattac
    26161 aacaaactgc gataacgcaa agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa
    26221 actacaatgt cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct
    26281 ttagggctgg cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct tacacttgac
    26341 cagcttcaac aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt ttataacggc
    26401 tggccacaaa ccttaaatgg cccttctggt catgaacctg ccgccttaaa cctctctgcc
    26461 agctggctcg acaaaatgag caccgaacag ctcaacgcgt ggatcaagca acataacctg
    26521 aaagccgatg ccccggtggc gctgtacggt aatgacaaag atgtcgacgc cgtcaaaacg
    26581 cgactgcaaa aagcaggttt tacgcatatc tccattctga gtgacgcgct aagcgaacct
    26641 tcccgtctac aaaaactgcc gcactttgag cagcaggttt atccgcaatg gctgcacgac
    26701 ctgcaacaag gtaaagaggt tacggcgaaa cctgccggtg actggaaagt cattgaagcg
    26761 gcctggggcg ctcctaagct ttaccttatc agccatattc ccggtgctga ctacatcgat
    26821 accaacgaag tggaaagcga accgctgtgg aacaaagttt ctgatgaaca actaaaagcg
    26881 atgctggcaa aacacggcat tcgccatgac accacggtca ttctgtacgg gcgtgacgta
    26941 tacgctggag cgcgtgtggc gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg
    27001 ctggatggcg gctggcaaac ctggtccgac gcgggactgc ctgttgagcg aggaacgcca
    27061 ccgaaagtga aagcggaacc ggatttcggc gtgaagatcc cggcacaacc gcagttgatg
    27121 ctcgatatgg aacaggcccg tggactgctg catcgccagg atgcatcgct ggtgagcatt
    27181 cgttcgtggc cagaatttat cggtacgacc agcggttaca gctatattaa accaaaaggt
    27241 gaaatagccg gagcacgttg gggacacgct ggtagcgact cgacgcatat ggaagatttt
    27301 cataacccgg atggcaccat gcgcagcgcc gatgatatta ccgctatgtg gaaagcgtgg
    27361 aatatcaaac cagagcagca agtttcattc tactgcggca ccggctggcg cgcgtccgaa
    27421 acctttatgt acgcacgcgc catgggttgg aagaatgttt ccgtgtatga cggcggctgg
    27481 tacgaatgga gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg cccggacagt
    27541 agcaaataac attgcaatta ctgacgctgg tgcgacttca gcgtcagata cccactccac
    27601 acccgcgtaa aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac
    27661 catgcaggga ataaacatcc cagcacaaac agtaagatcg tttctgtgcc ttcggttaac
    27721 ccgcccagat aataaaacga tttatgcgca taaccgggat tatcaatctg atgtttcgcc
    27781 gccagcgcag caaaagcgag aaaactgctg cctgtgccga taaacgcaaa cagcagccag
    27841 ccgcctgcca gggcgttttg ctgcggtgcg gcgaggataa acccaaaagg taccagcgcg
    27901 taaaaaagaa aatcgagaga aatatcaaga aagccgcccg cgtcggtaag ccctctgcgc
    27961 cgcgccagcg caccgtccag accatcaagc agcctgttca acaaaatgac gacaagcgcc
    28021 gccagatacc agcccagcgc cagaaaaggc agtgccagca cgccgatggc aaatcccact
    28081 aatgttaaac catctggcgt aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac
    28141 aacggtttaa tccggggatg aagatggcgg tctagcacag gcactcctta aatataaagc
    28201 ctttctgatt gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta
    28261 atttatgaaa atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact
    28321 cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg gagtttgccg gtggtaaagt
    28381 cgagccggat gaaagccagc ggcaggcgct ggtgcgtgag ttacgcgaag aactgggcat
    28441 cgaagcaact gtgggtgagt atgttaccag ccatcagcga gaagtttcgg gccggattat
    28501 ccatcttcat gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca
    28561 ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat
    28621 tccattatta gaggcgttta tggctttacg cgccgccaga ccagcggatt agtgctaagg
    28681 gttttgtcat cccgctggca ttgcagcagt attccctcgg ctttaattac cgcgccttca
    28741 gaataatttt tatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct
    28801 gaactgaaca cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc
    28861 agtgcaatta atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc
    28921 ttaacgttat tgtctctgct actgataacg gtagccgggt ggcaaaactt tagcgtctga
    28981 gttatcgcat ttggttatga gattactctc gttattaatt tgctttcctg ggtcattttt
    29041 ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatcacata
    29101 aacaacataa gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat
    29161 tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg
    29221 caagccgttc gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat
    29281 cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg
    29341 gtatgggttg atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt gcagttcagc
    29401 tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctctccatt
    29461 cttaaattcc tcggcttcga acaaaccttc aaaaatgccc tgactaccct gccgatgggc
    29521 ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga agtgatgcgt
    29581 ttttgccagg cgctgatgac tgaactgtat cgccacctgg gcgcggatac cgacgttccg
    29641 gcaggtgata tcggggttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag
    29701 ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggcagtctt
    29761 attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat gctaaaacgc
    29821 cacggtatgg gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag
    29881 tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc
    29941 ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc
    30001 aaagccagcc gcgatggtcg agtggcagat tatgccaaag aatttggtct ggtctatctc
    30061 gaacgccaac agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac
    30121 gaactggatg ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgccgaa
    30181 ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg
    30241 tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa cgtcaggcct tgaaatggcg
    30301 caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc
    30361 atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac
    30421 gtgcagggcg cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt
    30481 gtgatttaag ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa atgggcacaa
    30541 ttcattgcag ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca
    30601 ggcgtttatg agtatttaac ggatgatgct ccccacggaa catttcttat gggccaacgg
    30661 catttcttac tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc ttcaagttca
    30721 gcatccgtta actttctgcg atagcagcag atatgccagt aaagaaaccc cattagacta
    30781 tttttttgat aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt
    30841 atataatgcc aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc
    30901 gaggtcatcc atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga
    30961 ttattagcgc aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg
    31021 ataacctgct ctggggcttc ctccatgttt gctttaaaga tattggctcc atggtcgcca
    31081 gaaagaaaat gctccattaa ggcacaataa ctttcgctat cttcgatacc ccattgatcc
    31141 tctaaagact cgcgtctttt acttatgata tcgatcgagt caaagggaag cacatgatat
    31201 tggaaggtat ctttgccagg ttcaggcttt cgcggccaga actccagcgt ttcagaccat
    31261 tgcttatgat agaaccgata aggtgcgatc aattgtagcg cctgtaactt ctcgatactg
    31321 agcggctcaa tacctttagc ctgataataa tgcagttgtt ctttttttgc tttaaaaccg
    31381 gcccgaacaa taagccccat cataattaat agataaagaa aagagcatcc cgcggtaatc
    31441 aggcctcttt cattcaaacc gttggatgtt atcgctgcga acacaaacat tacagcaaca
    31501 acacatgtta aataaaaccc ccacttacaa agcagcatgg ccttattttc tttaatcatc
    31561 cgttcaaaat tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga
    31621 tggttttgta ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat
    31681 aattttatcc atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga
    31741 atatgactcg aatagcacga aagattcact cgcttacgct atcgccccgc ttccgacttc
    31801 atctgctggc ggactttttt tcgcactacg tttacgcggt gcagcctttt tcttatcagc
    31861 actgccacca ctgcccggag ccacaatgcc gcgaaactgc cgcaccggcg tacgtttggc
    31921 ttgatcaata agctgatata gcgtccccac cagcggctgc ataaagtcct gatagcgaca
    31981 ctgcttttcg ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt
    32041 cgccatttct ggcagcgaat ggaataacgc ttttccggcg tcggtagagt ggatatagcg
    32101 cccttttttg gtcagaaaac cacgcttgaa cagcagttca ataatcccgg cacgggttgc
    32161 ttctgtcccc agaccatcgg tcgcacgaag gatctttttc agatctttgt cctgcacaaa
    32221 gcgcgcgatc ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg
    32281 ggtttgccgc tctaccactt cacctttttc acacagcaac tcatcgcctt tcgccaccac
    32341 aggcaatggc gtaccgtcgt tttcttcatc gcgctctttg ctgcctaaca gcgtgcgcca
    32401 gcctgcttca gcaagaaaac gggctttagc gacaaatttg cctttggcaa tgtccagttc
    32461 gataacacac ttgcggaaca ccgcatccgg gcagaactgc atcaggtatt gacgggcaat
    32521 caggttatag accttcgctt cgttctccgt caggttgatc gcagaactcc gtgcggtcgg
    32581 gatgatggcg tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc gtatatctgg
    32641 atctaccact gactgcggca acagatccgg tgcatgaacg ctgatggcat tcatcaccgc
    32701 gtggcgtccg gcaaaatgtt cttctggcaa atagcgacaa tcggaacgcg gataagtgat
    32761 tagcttgtgc gtttcgtaca gcttctggca gatatcaagc acgttctgcg cgctcagacc
    32821 gaagcgtttt gccgcttcaa tctgcaacgc cgaaagcgaa aacggcagcg gcgcggattc
    32881 tgattcccgt ttatcgttat agctggtgac aatagccggt tgaccactaa tgcggttaac
    32941 cacatgctcc gccagtggac gatgtaacaa gcgcccttct tcatcctggt acggttcaca
    33001 cgcttcgctc ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc
    33061 tttgacttca aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac
    33121 cagcccgagc acgggtgtct gcacgcgtcc cacggaaagt acgccctgat aaccggcatt
    33181 gcgaccgaga atggtatacg cacgggtcat attgatgccg tacagccagt cggcacgcgc
    33241 tcgcgccagc gcagaaacgc acagcggtac aaactcactg ttggaacgaa gacggtcgat
    33301 cgcccgctca accgcctgcg ggttcaggtc gtttatcaag caacgctgca cctgctggcg
    33361 cttttccggt gccagttgca gatagtccag cacttcatcc accagcaatt gcccttcacg
    33421 atccgggtcc ccggcgtgaa cgatttcgct ggcttcatgc aggaagcgtt tgatgacgtt
    33481 aagttgtttg gtcacggagg gtcggggctg taattgccac ttttccggga caatcggcaa
    33541 atccgcaaga ttccagcgcg catagcggct gtcgtaggcg tctggctgcg cctgctcaag
    33601 caggtgaccg atacaccagg tcaccacctg accattaccg cactcgataa agccatcgcc
    33661 tttacggtgc ggtttgggca ggacatcagc aatggcgcgc gccagactcg gtttttcggc
    33721 aataaacaac cgcattgaat taatggatct caaccatggc acgaccgccg cgcgctggca
    33781 ccagttcgcc aatcgccgtc agttcaatgc caaactcggc ggctgtagtt ttaacctcat
    33841 tttctgcttc cggcatgacc gccagcagca aaccgccaga agtttgcgga tcgcacagca
    33901 gatcgcgcac ttcacgcgga atttcaccca tcagatgacc gtagctggca aagttacgtt
    33961 cggtgccgcc aggcactgcg cccaacttaa tgtactcttc aacccccggc aattttggga
    34021 tcgcgtcata gtcaacgcgc gcctgcacac ctgcgccctg acacatttcg ctcaaatggc
    34081 ccagcagacc aaaaccagta acgtcggtca tcgcttttac gccttcgatg ttggcaaagg
    34141 acgcacctgc gatgttcatc cggcacatta cttccgtcgc cagtccctga tgttctggtt
    34201 tcaacagtga ttttttctca gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga
    34261 acagtttgca tccggcttgt gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc
    34321 ccgttaccgc cagaccaaaa atcggctccg gcgcatcgat ggagtgaccg ccagccagcg
    34381 caatacccgc ctgacggcat gcatagcgtc caccttcggt tacttcgcgg gcaatttctg
    34441 gggaaagttt gttaatcggc cagccgagga tcgcaatcgc cataatcggt ttgcccccca
    34501 tcgcgaagat atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat
    34561 caacgatcgg cataaagaag tcggtggtac tgataacgct ggtgccattg cccagatcgt
    34621 acaccgccgc atcgtcgcgg gtctcattac ccacaagcaa attcggatca acaaacttcg
    34681 cctgctcact gtgcaggatg gtttccaaca cttttgggga aattttacag ccgcaaccag
    34741 ctccgtggct gtattgggtc aaacgaatcg agttctcgct catggacatc tcctgtcaat
    34801 gcaatccggg tatggtaacc ctcattccgt gaagtgataa gtgagagtgt ctgaattcct
    34861 gcgcctttgc tcacaatcca gacagtttcg cgacaattat cagaaataag ttacaaacgg
    34921 cgtcgggtcc gggacgttaa tcgacgtaga tgctttcaac tgcggcgtgc cgaggtagag
    34981 aaaaccgaca attttatcct gctcacggca accgaatgct tcacgcacta ccggactttc
    35041 agttaatgcg ccactgcgcc agatgccgcc aaatccttgg gcaacggctg ccatttgcat
    35101 cgccatgacc gcgcatccgg cagacatttc ctgttcccat cgcgggactt tatgattttc
    35161 ttcgcatttc gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt
    35221 gtcgatagct ttgtcatcac taccggcagc aatcgccccc tgttccagta cggcgctgaa
    35281 acgctcgcgc ccttcccctt caatcacaaa aaaatgccac ggttgcatgg acttatggtc
    35341 cggcgcacgc atacccgcac gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc
    35401 agccaggcgg gaggcgctac ggcgattgat caatagttcg agtgcatcca tttgattaac
    35461 tcctgtcgtg atatttattc acaaaattaa cacgagagtg gattttgtta cagcacagtc
    35521 cgcaattcct gctgacaagt gccggttggg tcattacgat aaccacatct attgcgcctg
    35581 tgacaggtgt gaccttaagt tgggagaata catgcgaacc ctttggcgat ttattgccgg
    35641 attttttaaa tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt
    35701 ctttattttc ctcgtactgg ttggtgtggg gatctggatg caggtcagtg gtggtgattc
    35761 gaaagaaacg gccagtcgtg gcgcactgct gctggacatt tctggtgtga tcgtcgataa
    35821 acccgacagt tctcagcggt ttagtaaatt aagccgccag ctgcttggtg ccagttccga
    35881 tcgtctgcag gaaaactcac tgtttgatat cgtcaacacc attcgccagg cgaaggacga
    35941 ccgcaatatc accggtatcg tgatggatct gaaaaacttc gcaggcggcg accagccgtc
    36001 tatgcagtac atcggcaaag ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc
    36061 cgttggcgag aactacagcc aggggcaata ttatctcgcc agtttcgcca ataaaatttg
    36121 gctgtcaccg caaggcgtgg ttgatctgca cggttttgct accaacggtc tgtactacaa
    36181 atcgttgctg gataagctga aagtttccac ccatgtgttc cgcgtgggta cgtataaatc
    36241 tgccgttgaa ccgtttattc gtgatgatat gtcaccggca gcccgcgaag ctgacagccg
    36301 ctggattggt gagctgtggc aaaactatct gaatactgtt gccgctaacc ggcagatccc
    36361 tgctcagcag gtattccctg gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg
    36421 cgataccgcg aaatatgcac tggaaaacaa gctggtcgat gcactggcat cgagtgccga
    36481 aatcgaaaaa gctctgacca aagagttcgg ctggagtaag actgataaaa attatcgcgc
    36541 catcagttat tacgattacg cattgaaaac gccggcagat accggtgaca gcatcggtgt
    36601 cgtcttcgct aatggcgcaa ttatggatgg cgaggaaact caggggaatg ttggcggtga
    36661 taccactgcg gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct
    36721 gcgtgttaat agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc
    36781 agcagcccgg gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg
    36841 tggttactgg atttccacgc cagctaatta cattgtggct aaccccagca ccctgaccgg
    36901 ttctatcggt atcttcggcg tgatcaccac cgtagaaaat agtctggatt cgattggtgt
    36961 tcatactgat ggtgtctcaa cttcaccgct ggcggatgtt tctatcacca gggcactgcc
    37021 gccggaagcg cagcagatga tgcaattaag cattgagaat ggctataaac gctttatcac
    37081 gctggttgct gatgcgcgtc attcgacgcc ggagcaaatt gataaaatcg cccagggcca
    37141 cgtctggacc ggtcaggatg caaaagctaa cgggctggtc gatagtctcg gggatttcga
    37201 tgatgcggtt gccaaagcag cagagctggc aaaagtgaaa cagtggcatc tggaatacta
    37261 cgttgatgaa ccgaccttct tcgacaaagt gatggacaac atgtctggtt ctgtccgggc
    37321 aatgttgcca gatgcgttcc aggccatgtt acctgcaccg cttgcctcgg tagcctctac
    37381 cgttaaaagt gaaagcgaca agctggccgc gtttaacgac ccacaaaacc gttatgcgtt
    37441 ttgcctgacc tgcgccaacg tgcgttaagt cttgtactga gtggccgaca gatcgtcggc
    37501 cacattattt tttacctcga cgaatcctct tcccgctgtc tcgacccata tttccttatc
    37561 cgcatagtat caggtgcgct cctcctgcct cacgtatata cttttgctct ttcgatatca
    37621 ttcatatcaa tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg
    37681 ggatgcagcg ttccgagcag ggttatattc cggtgtcagg tcatctacaa cgccaactgg
    37741 cgctgatgcc ggaattccat cgcccggaga tgccagattt caccattcat gaatatacgc
    37801 cgttgatgga ttcttctgat atgacgccgg aagactggca gcatattgct gaagatatta
    37861 aagcgcacta tgacgactat gatggttttg tcattctaca cggtaccgac acgatggcgt
    37921 ataccgcctc tgcgctgtcg ttcatgctcg agaatctcgg taaaccggtc attgtgacag
    37981 ggtcacaaat cccgctggct gagttacgct ctgacggaca aattaatctg ctgaatgcgt
    38041 tgtacgttgc ggcgaattat ccgatcaacg aagtaacgct ctttttcaat aaccgattgt
    38101 atcgcggcaa ccgcactacc aaagcccatg ccgatggttt tgatgcgttt gcctctccaa
    38161 accttcctcc gttactggaa gctggtatcc atattcgtcg tttgaatacg ccacccgccc
    38221 cgcacggtga aggggaatta atcgttcatc caatcacgcc acaaccaatt ggcgtagtga
    38281 cgatttatcc agggatttct gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag
    38341 cattgattct gcgctcctat ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg
    38401 aattacaaga agccagcgat cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg
    38461 gtaaagtgaa tatgggtggt tatgccaccg gtaacgccct cgcccatgcc ggcgttattg
    38521 gcggtgcaga tatgactgta gaagccacgc taaccaaact gcattacctg ctgagccagg
    38581 aactggatac tgaaaccatt cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc
    38641 cggatgatta aggagactgc aatgccccat cgcgccctgt tactggtcga tttacaaaat
    38701 gatttctgtg ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt agatgtcgct
    38761 aaccgcctga ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg
    38821 cacccggcga atcacagcag ttttgccagt cagcacggtg tagagcctta tacgccaggc
    38881 caactcgacg gtttgccaca aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc
    38941 gcacaattac atccgttact gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa
    39001 aatcctttag ttgacagtta cagtgccttt tttgataacg gccgtcggca gaaaacctct
    39061 ctcgatgact ggttacgcga tcatgaaatc gatgaattga tcgttatggg cctggctact
    39121 gactattgcg tgaagtttac cgtgctggac gcgttacagt taggttataa ggtaaacgtg
    39181 attaccgatg gttgtcgtgg cgtgaatatc cagccccagg acagtgcgca cgcgtttatg
    39241 gagatgtcag cagctggggc aacgctatat acgctggcag actgggaaga gacgcagggg
    39301 taatattagt tgaattcatc tctggcctac atttcctcaa tgacattgta ggccagataa
    39361 tacttgtcag tgttgcattt aacctacttg cagctaaaac acgacgtcct aacaagtata
    39421 tttgccctcc cttaataaca ggcttaaata ctagataact cattcactta aacatagcaa
    39481 taggctttgc tgagatacca aaatcttctt ttaactgctg tttgctcttc atgaccatct
    39541 gaccatttgt gtcaattgtc atatgctgtg cttcggtatt atggcgggct tgccacagca
    39601 tcaccagttg caggcaattc tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt
    39661 tccccagttc aacggcggtc gacaaacgct ggtatacctc aggcgtcata ctgttgataa
    39721 tgtcatcaag attcataatt tttccgctcc tgtggaataa gttgctgaat cgttttttca
    39781 accgttgatt tcttcgccgt tttcgccatc agtgaagcgt aaagaagcag agttaacaca
    39841 ataacgttcg cctgttggtt gcggcccgtc gggaaagaca tgccccagat gggcatcaca
    39901 gttaccgcaa cgaatttcta tgcgctgcat tccatgtgac aagtctttga tataacgaat
    39961 ggattcttca ctcaccggtt cgtagaaact gggccagcca cagccggaat catacttggt
    40021 ttcggaatga aacagcgggg catcgcagat caaacagtga tatacgccgt cacgcttgtt
    40081 atgcagtaaa caacccgtaa atggcggttc tgtcccatga ttctgcgtca cgtaaaactg
    40141 catctcggac aaattttttt tcagttcttc tgccgaaggt ttattagcca tttgctcaca
    40201 tctcacttta atcgtgctca cattacgtga ctgattctaa caaaacatta acaccaactg
    40261 gcaaaatttt gtcctaaact tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa
    40321 tcacattttt atcgtaattg ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag
    40381 cccaaaggaa gagtgaggcg agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg
    40441 caatgattga cacgattccg cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc
    40501 ttttattcac taacaaatag ctggtggaat atatgactat caaagtaggt atcaacggtt
    40561 ttggccgtat cggtcgcatt gttttccgtg ctgctcagaa acgttctgac atcgagatcg
    40621 ttgcaatcaa cgacctgtta gacgctgatt acatggcata catgctgaaa tatgactcca
    40681 ctcacggccg tttcgacggt accgttgaag tgaaagacgg tcatctgatc gttaacggta
    40741 aaaaaatccg tgttaccgct gaacgtgatc cggctaacct gaaatgggac gaagttggtg
    40801 ttgacgttgt cgctgaagca actggtctgt tcctgactga cgaaactgct cgtaaacaca
    40861 tcaccgctgg tgcgaagaaa gtggttatga ctggtccgtc taaagacaac actccgatgt
    40921 tcgttaaagg cgctaacttc gacaaatatg ctggccagga catcgtttcc aacgcttcct
    40981 gcaccaccaa ctgcctggct ccgctggcta aagttatcaa cgataacttc ggcatcatcg
    41041 aaggtctgat gaccaccgtt cacgctacta ccgctactca gaaaaccgtt gatggcccgt
    41101 ctcacaaaga ctggcgcggc ggccgcggcg cttcccagaa catcatcccg tcctctaccg
    41161 gtgctgctaa agctgtaggt aaagtactgc cagaactgaa tggcaaactg actggtatgg
    41221 cgttccgcgt tccgaccccg aacgtatccg tagttgacct gaccgttcgt ctggaaaaag
    41281 ctgcaactta cgagcagatc aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag
    41341 gcgttctggg ctacaccgaa gatgacgtag tatctaccga tttcaacggc gaagtttgca
    41401 cttccgtgtt cgatgctaaa gctggtatcg ctctgaacga caacttcgtg aaactggtat
    41461 cttggtacga caacgaaacc ggttactcca acaaagttct ggacctgatc gctcacatct
    41521 ccaaataagt tgagatgaca ctgtgatcta aaaagagcga cttcggtcgc tctttttttt
    41581 acctgataaa atgaagttaa aggactgcgt catgattaag aaaatttttg cccttccggt
    41641 catcgaacaa atctcccctg tcctctcccg tcgtaaactg gatgaactgg acctcattgt
    41701 ggtcgatcat ccccaggtaa aagcctcttt tgcattacag ggcgcacacc ttctctcgtg
    41761 gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg
    41821 cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg tttggtccgg cggcacaaca
    41881 aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga
    41941 agatgctgat ggcgtagcgc tgacttttga attgacgcaa agcgaagaga cgaaaaaatt
    42001 ctggccgcac gactttacgc tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga
    42061 tcttgaatca catggcgaat ttgaaaccac ctctgccctg catacctact ttaacgtggg
    42121 tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc ttcattgata aagtaaatga
    42181 cgcgaaagaa gatgtactga ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt
    42241 gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc
    42301 cgtaggccac cagcatcatc tgaacgttgt cggctggaac ccgggaccgg cgctttcagt
    42361 tagcatgggc gacatgccgg atgatggcta caaaacattt gtttgtgtag aaacggctta
    42421 cgcttcagaa acgcaaaaag tgaccaaaga gaaacctgca catctggcgc aatccattcg
    42481 cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta
    42541 tcaacatcac accatatcca gcgcagtttt tccttttggt gccggatacg ctttatccag
    42601 catggttaat tccgctgacg aaagttcgac ctcaagcaca gccgcattct gttggacatg
    42661 ggcaatcgtg gccgcttttg gaatcgccat cacaccctga tggctgatta cccacgccaa
    42721 caatacttgt gccgcgctga tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt
    42781 taacagtcca ttgcgcaacc gcccggcctg ggctaatgga ctgtaagcca tcaccggcat
    42841 ctgctgttgc tggcaccagg ggagtagatc gtactcaatc cctcgtgaac cgagatggta
    42901 aagcacctga ttagtggcac actgatttcc ccccggcagc tgccagagtt cctgcatatc
    42961 agcatgatca aggttagaaa cgccccagcg gcggattttt ccctgggcga tcaatttttc
    43021 catcgctgcc acagtctctt caaaagcgaa actgccagac cagtgtaata agtagagatc
    43081 gagataatca gtattgagac ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc
    43141 gccagcattc cacggataga ctttagagac gagaaagacg ttatcgcgca gaccggttaa
    43201 tgcttcccca accacctttt cggcaccgcc atcggcatac atttcggcgg tatcaatgag
    43261 ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact tctgttttgc gctgactggc
    43321 atcttcgccc atataccatg ttccctgccc tatggctggc agtgagacat cgccactaaa
    43381 ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg caccaccgta atgcaaaaca
    43441 gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa agatgcatta tcagaatttg
    43501 taggtgatcc cggtagaaat caggccagtc caggatttat ccaccatcgg gctgtcagta
    43561 acttcatcag acagacgggt atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag
    43621 ttgtagctgg cgctcagctc caggtaaggg ctccagctgt cgttcgggtt atagccacgc
    43681 agaccgctgc gagcggactc tttgcgcgat acgccataat agtattcgtt ctggttttcg
    43741 ctgttccact gcacaccgat acccggggtc acggtcaggc cgccgttggt gtaacgatac
    43801 aaccaggcca tatcccagac gatgccgttg ctgttatcca gggtatcgcc agccagggtg
    43861 gtacgcaggt aaccgtactg ggtaaagtga gcataagaca gaccagccat catggtgctc
    43921 ttacggtcat ccaggtgacg catttggtga tcgccactgt ctttcgcttt gaagtaaagc
    43981 ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt cattccacag gtagtaacca
    44041 ccacctaagc cacggaacca gaagttatcg ccttcatagt tgattaccgg tactgggtaa
    44101 acatcggtat cgtaatcttt atatgggtgc tcaacgactc ctacgcctgc gcccagggaa
    44161 aatttacctt cagcgtgcgc tacgcctgca gacgttgcga taagcactcc aagtgccaga
    44221 agtttgagtt tggtcacaat taatcattcc ttaaacaaat gtttagcggg cgacaaagtt
    44281 tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc attttttaaa gtaactgttt
    44341 aattttcctg acgcggatga caccgcgctt acagccaaat gaatttagcg ttactggcta
    44401 ccctggtctt tacattaatt atgcaaaatt tatggatgag ttgttgatat gccattgaaa
    44461 ttaagaaagc cgtgcaggca agttttccat ttgccatcta cgcttaattt tgaaggtgta
    44521 tcaccgggca cgttgttctc atcgtcgata aaatggcatg agagttgctg tgttttagca
    44581 agagacgtcg ttcagtttac ctcttccggg agcctctact attcatatga acggctctta
    44641 acctgtgcta aaaaacgaaa ggacggcata ccatgaatat attcgatcac tatcgccagc
    44701 gatatgaagc tgccaaggac gaagagttca cactgcagga gtttcttacc acttgtcggc
    44761 aagatcgcag tgcttatgcc aacgcggctg agcggctatt gatggctatc ggtgagcctg
    44821 tcatggtcga tacagcccag gaacccagac tttctcgact cttttctaac cgggtcattg
    44881 cacgttatcc ggcatttgaa gagttttacg gcatggaaga cgcgattgaa cagattgtct
    44941 cttatctgaa acacgcggct caggggctgg aagagaagaa acaaatcctg tatctgctgg
    45001 ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact gaaatcatta atgcagctcg
    45061 taccgattta tgtattgagc gccaacggtg agcgtagccc ggtcaacgat catccgttct
    45121 gtcttttcaa tccgcaggaa gatgctcaga ttctggaaaa agagtatggc attcctcgcc
    45181 gttatctcgg caccatcatg tcgccatggg cggcaaaacg cctgcatgaa tttggtggcg
    45241 atatcactaa gttccgggta gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg
    45301 ccaaaacgga acccggtgat gagaacaacc aggacatctc cgcgctggtt gggaaagtcg
    45361 atattcgtaa actcgaacac tacgcgcaga atgacccgga cgcctacggc tattccggtg
    45421 cgctgtgccg cgccaaccaa gggatcatgg aatttgtcga gatgtttaaa gcaccgataa
    45481 aagtgctgca tccgctgtta accgccactc aggaaggtaa ctacaacggg acggaaggta
    45541 tctccgccct gccgttcaac gggattattc tcgcccactc gaacgaatcc gaatgggtca
    45601 ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg tgtttacatc gtgaaggtgc
    45661 cgtattgctt acgcatttcc gaagagatca aaatctacga gaaattgctt aatcacagtg
    45721 aattgactca cgccccctgc gcccctggca cgctggaaac gctgtcacgt ttttccattc
    45781 tttcgcgcct taaagagcca gaaaactcca gcatttattc aaagatgcgg gtttatgatg
    45841 gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta tcaggaatat cgtgactacg
    45901 ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt tgcgtttaag atcctctccc
    45961 gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc
    46021 tggaacagca gatcgagcgc gagcagttcc cacaagagca ggcagaacgc tatctggagt
    46081 tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg
    46141 cctaccttga atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg
    46201 cggatttctg gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc
    46261 gcgagtctct taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa
    46321 aagatttccg caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac
    46381 gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct
    46441 ccaataccga ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc
    46501 agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg
    46561 tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg
    46621 gtacacatgt taccgggcct acaacgacag cgaaccgtgg gcctgagaag cggcaacaca
    46681 ggcgtagcat acagttggca aatgtagtac ggggggcata tgacctggtt tattgaccgg
    46741 cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa
    46801 gcgcaaatta aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac
    46861 agcggcgaat ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg
    46921 cgtggcggtc tgcgccaccg cgtgcatccg ggcaatgacc atttcgtcca gaacgaccga
    46981 attgaacgtc cccagggtgg cggcggaggt tccggtagtg gtcagggcca ggccagccag
    47041 gatggtgaag gtcaggatga atttgtcttt cagatttcga aagatgagta tcttgatttg
    47101 ctctttgaag atttggcctt accgaatctg aaacaaaacc aacaacgcca gctgaccgaa
    47161 tataaaacgc atcgggcggg ttataccgct aacggcgttc cggcaaatat cagcgttgtg
    47221 cgctccttgc agaactcact ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa
    47281 cttcatgcac tggaagagaa tttggccatc atcagcaaca gtgaacctgc gcagctgctg
    47341 gaagaggaac gtctgcgcaa agaaattgca gaattacgtg ccaaaattga acgcgtccct
    47401 tttattgaca ccttcgattt acgttacaag aactacgaga agcggcccga tccctccagc
    47461 caggcagtga tgttttgcct gatggacgtt tccggttcaa tggatcaatc cactaaagat
    47521 atggctaagc gtttttatat tctgctgtat ctgttcctca gcagaacgta taagaacgtg
    47581 gaagtcgtat acatccgcca tcatacccag gcgaaagaag tcgatgaaca tgagtttttc
    47641 tactcgcagg aaacaggcgg caccattgtt tccagcgccc tgaaactgat ggatgaggta
    47701 gtgaaagagc gttataaccc ggcacagtgg aatatttacg ctgcacaagc atcggacggc
    47761 gataactggg ccgatgactc tccgctttgc catgaaatcc tggcgaaaaa attattacct
    47821 gttgttcgtt attacagcta tatcgaaatt acccgtcgtg cacatcagac attgtggcga
    47881 gaatatgagc atctgcaatc tactttcgac aactttgcga tgcagcacat ccgcgaccag
    47941 gatgatattt atccggtgtt ccgtgaactg tttcataaac aaaatgcaac agctaaagac
    48001 taaaactatc agccaggtca ttatcgcctg gctgattttt agcttactgt aaactatctc
    48061 atttattaca tacattagct tacaatcgct ttaaatatga cagcgtaacc tttacataat
    48121 ttagttccag aaaacaatca ttcggaaaaa tgattcagtc aacacgtatt tccatggggt
    48181 tattctttaa atattgttta tcgttaacga aaattgatcc tggtcaaaac tatatatctc
    48241 tgccatcaat aaaatccagc actcacattg ctctcctttt tatggtttct atgggtacac
    48301 aaaaattaaa agctcaaagc ttttttattt tcagtttatt gctgacgtta attttatttt
    48361 gcatcaccac attatataac gaaaacacaa atgtaaaact catcccacag atgaattacc
    48421 tgatggttgt tgtggctttg tttttcctta acgccgtcat ttttcttttc atgttaatga
    48481 aatatttcac taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg
    48541 gccttatcta tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac
    48601 tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta
    48661 tttgtttaac ctcgctggca ctcttttgtt atggaaaaga caacatcctt gacaacaata
    48721 agaaaaaaac gggaatcctg ttgctggcgc tgattccttt tttagttttt ccccttctgg
    48781 cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgac tactgtccgg
    48841 acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat
    48901 ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag
    48961 cattattatg tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata
    49021 attacaccat ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt
    49081 cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg
    49141 atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt
    49201 cgcgacctgt tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca
    49261 tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta
    49321 tccagcaaag catccgccct gatgatattt tagcgcgact ggagggtgag gtgtttggct
    49381 tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa
    49441 atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca
    49501 gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag
    49561 cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata
    49621 tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta
    49681 ctattacccc taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg
    49741 tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat
    49801 ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc
    49861 tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc
    49921 tgcgctatat cgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga
    49981 gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac
    50041 agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga
    50101 ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca
    50161 caattgtgga agctgcagca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc
    50221 accaggatga ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg
    50281 atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgcgatga
    50341 ataactggtc cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg
    50401 tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact aaacagaacg
    50461 tgatgagttt gctgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg
    50521 atattaacaa aaacaatttg cggaatatct tttacactca tgaccgccct ctcctctggc
    50581 gttttctcaa tgtcacgcta accgataccg attcggggcg cgacattatc atcaaccaga
    50641 gcgaagataa tctgttccag tacgtcagtt acgtccatga cttaccgggc ggcattcgtg
    50701 tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct
    50761 ggattttggc ggcgttaatt ttgctgaata tggtccggat gcacttccgt ttataccaaa
    50821 atgtgtcgcg agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa
    50881 cccctgaact ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta
    50941 ttgcaattga catggacaag ttaaagcaaa taaatgacac cctcggtcat caggaggggg
    51001 atttagcgat tacgttatta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg
    51061 ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg
    51121 cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaaa
    51181 tcggcttctc ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata
    51241 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
    51301 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
    51361 gcgtgacgca tcagcggtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
    51421 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctctag
    51481 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
    51541 tggcgatcct cgctgccgat caacaggccg atctgagcca actcgccagc catataggcg
    51601 gattacgcgc ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg
    51661 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
    51721 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
    51781 aaaccacgga ttatttgcgc attgcgcaac cagaactgct caatttccgc cgcactgcgt
    51841 aactagcctg tccgttcaat aagcagaacg gacaaaacca gcacaacagc aatggcaaaa
    51901 aacgatgacg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
    51961 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
    52021 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
    52081 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
    52141 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
    52201 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
    52261 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
    52321 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
    52381 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacctt aatgggcagc
    52441 cagccgcaac tggtcgccgg gctgctggta ggcactgttt taggcgtggc gctgtttcgc
    52501 ggcgtaccgg tcggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
    52561 tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa
    52621 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
    52681 tcactaatcc ctgaagtgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
    52741 accccgtctc ttttactatc agtcgcgcaa gattgcgttc actcatcgca aaaaagccag
    52801 cccattgccc taatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
    52861 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
    52921 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
    52981 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
    53041 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
    53101 tcacttgatt gctatgttcc accccgcccg gtatccaaac cgcatattgc ggcggcacca
    53161 tccacaaagc attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc
    53221 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
    53281 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
    53341 ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
    53401 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
    53461 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacta cattacgcgt
    53521 caccttcacc ggcgcagcac cgttactcga tacgattcgt tccgcttact cgctgacgac
    53581 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcaccttt
    53641 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
    53701 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
    53761 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
    53821 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
    53881 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
    53941 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
    54001 gcgaagccaa caacatgcaa atttgagtac ctcgcgcgcc ttacatactc ggggtatctg
    54061 gcgttcgccg cttgcctggc aggtcacatt gtttcttggg atcaactcac tggtctatta
    54121 cgtgattatt ggctggcttc cggcgatcct catcagtcgc ggctatagcg aagcacaggc
    54181 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatccctct
    54241 tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc
    54301 agtgggcgct gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
    54361 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
    54421 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
    54481 agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc
    54541 actgatgggt gttgccatac tttcactact gatggcgatt ttcggacttt gcgccgggag
    54601 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatcat gcgtgaaagg
    54661 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
    54721 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
    54781 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
    54841 cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga
    54901 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctacgc
    54961 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
    55021 attttagtac agcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
    55081 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
    55141 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
    55201 taccaatctg cggcggttta ggtgcttctg gctgagaaga ggttgaacaa ccagccagga
    55261 ccagcaagca aggcaaagca aaactgataa ttttcattta ttgatctcac acatttatct
    55321 acgcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
    55381 aaacatatta cgtcttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
    55441 aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc
    55501 cgcgtctcgc aatcccttgc caaagaacag tcactagaaa gtctggtccg acagcttctg
    55561 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
    55621 gcgcgcctgc agcatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
    55681 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcta ttgatgaaaa ctgctttttc
    55741 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggaatcacc
    55801 acatttctga gtacaccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
    55861 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc
    55921 ggactgattg cacaatatat tcaaaaagag gcgttggttg aacagctgcg cgaagccaat
    55981 gccgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
    56041 atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca taagataatg
    56101 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg atcgttttgg tcataatagt
    56161 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
    56221 gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaataa cgagaatgcg
    56281 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt
    56341 gatgttgatt tgtattatcc tggtgccagt cttggcattg tagaagtcga tcctgaaacg
    56401 accgatgcag acagtgccct gcatgctgcc gatatcgcga tgtatcagga gaaaaaacac
    56461 aaacagaaaa caccttttgt cgcgcattca gcgccacatt cctgaggcgt attcacatcc
    56521 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc tttattacag
    56581 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
    56641 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct
    56701 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
    56761 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
    56821 acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagcgtg
    56881 ctgatccatc cgccaactac ggcaccgact atccccagca ggatagtcat aaagaatcca
    56941 ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata
    57001 atccatgaca gaat
//
LOCUS       DAZUFB010000020        54599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZUFB010000020 DAZUFB010000000
VERSION     DAZUFB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.020"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            315..401
                     /gene="leuL"
                     /locus_tag="ACHY3M_001413"
     CDS             315..401
                     /gene="leuL"
                     /locus_tag="ACHY3M_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFS4316354.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            494..2065
                     /gene="leuA"
                     /locus_tag="ACHY3M_001414"
     CDS             494..2065
                     /gene="leuA"
                     /locus_tag="ACHY3M_001414"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFS4316355.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2065..3156
                     /gene="leuB"
                     /locus_tag="ACHY3M_001415"
     CDS             2065..3156
                     /gene="leuB"
                     /locus_tag="ACHY3M_001415"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFS4316356.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3159..4559
                     /gene="leuC"
                     /locus_tag="ACHY3M_001416"
     CDS             3159..4559
                     /gene="leuC"
                     /locus_tag="ACHY3M_001416"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFS4316357.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4570..5175
                     /gene="leuD"
                     /locus_tag="ACHY3M_001417"
     CDS             4570..5175
                     /gene="leuD"
                     /locus_tag="ACHY3M_001417"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFS4316358.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5224..6402)
                     /gene="setA"
                     /locus_tag="ACHY3M_001418"
     CDS             complement(5224..6402)
                     /gene="setA"
                     /locus_tag="ACHY3M_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFS4316359.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6504..6635)
                     /gene="sgrT"
                     /locus_tag="ACHY3M_001419"
     CDS             complement(6504..6635)
                     /gene="sgrT"
                     /locus_tag="ACHY3M_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFS4316360.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6724..8379
                     /gene="sgrR"
                     /locus_tag="ACHY3M_001420"
     CDS             6724..8379
                     /gene="sgrR"
                     /locus_tag="ACHY3M_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFS4316361.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8413..8512
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8543..9526
                     /gene="thiB"
                     /locus_tag="ACHY3M_001421"
     CDS             8543..9526
                     /gene="thiB"
                     /locus_tag="ACHY3M_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFS4316362.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9502..11112
                     /gene="thiP"
                     /locus_tag="ACHY3M_001422"
     CDS             9502..11112
                     /gene="thiP"
                     /locus_tag="ACHY3M_001422"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFS4316363.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11096..11794
                     /gene="thiQ"
                     /locus_tag="ACHY3M_001423"
     CDS             11096..11794
                     /gene="thiQ"
                     /locus_tag="ACHY3M_001423"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFS4316364.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11908..12672)
                     /gene="yabI"
                     /locus_tag="ACHY3M_001424"
     CDS             complement(11908..12672)
                     /gene="yabI"
                     /locus_tag="ACHY3M_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS4316365.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12758..13636)
                     /gene="araC"
                     /locus_tag="ACHY3M_001425"
     CDS             complement(12758..13636)
                     /gene="araC"
                     /locus_tag="ACHY3M_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFS4316366.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13975..15675
                     /gene="araB"
                     /locus_tag="ACHY3M_001426"
     CDS             13975..15675
                     /gene="araB"
                     /locus_tag="ACHY3M_001426"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFS4316367.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15686..17188
                     /gene="araA"
                     /locus_tag="ACHY3M_001427"
     CDS             15686..17188
                     /gene="araA"
                     /locus_tag="ACHY3M_001427"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4316368.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDNPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17357..18052
                     /gene="araD"
                     /locus_tag="ACHY3M_001428"
     CDS             17357..18052
                     /gene="araD"
                     /locus_tag="ACHY3M_001428"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4316369.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18127..20478
                     /gene="polB"
                     /locus_tag="ACHY3M_001429"
     CDS             18127..20478
                     /gene="polB"
                     /locus_tag="ACHY3M_001429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFS4316370.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPPLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20643..23549
                     /gene="rapA"
                     /locus_tag="ACHY3M_001430"
     CDS             20643..23549
                     /gene="rapA"
                     /locus_tag="ACHY3M_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFS4316371.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALVREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23561..24220
                     /gene="rluA"
                     /locus_tag="ACHY3M_001431"
     CDS             23561..24220
                     /gene="rluA"
                     /locus_tag="ACHY3M_001431"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFS4316372.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24337..25152)
                     /gene="djlA"
                     /locus_tag="ACHY3M_001432"
     CDS             complement(24337..25152)
                     /gene="djlA"
                     /locus_tag="ACHY3M_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFS4316373.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25407..27761
                     /gene="lptD"
                     /locus_tag="ACHY3M_001433"
     CDS             25407..27761
                     /gene="lptD"
                     /locus_tag="ACHY3M_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFS4316374.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYRNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27814..29100
                     /gene="surA"
                     /locus_tag="ACHY3M_001434"
     CDS             27814..29100
                     /gene="surA"
                     /locus_tag="ACHY3M_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFS4316375.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29100..30089
                     /gene="pdxA"
                     /locus_tag="ACHY3M_001435"
     CDS             29100..30089
                     /gene="pdxA"
                     /locus_tag="ACHY3M_001435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFS4316376.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRSYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30086..30907
                     /gene="rsmA"
                     /locus_tag="ACHY3M_001436"
     CDS             30086..30907
                     /gene="rsmA"
                     /locus_tag="ACHY3M_001436"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4316377.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30910..31287
                     /gene="apaG"
                     /locus_tag="ACHY3M_001437"
     CDS             30910..31287
                     /gene="apaG"
                     /locus_tag="ACHY3M_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFS4316378.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRTPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31294..32142
                     /gene="apaH"
                     /locus_tag="ACHY3M_001438"
     CDS             31294..32142
                     /gene="apaH"
                     /locus_tag="ACHY3M_001438"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFS4316379.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32167..32766
                     /locus_tag="ACHY3M_001439"
     CDS             32167..32766
                     /locus_tag="ACHY3M_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFS4316380.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32802..33269
                     /locus_tag="ACHY3M_001440"
     CDS             32802..33269
                     /locus_tag="ACHY3M_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316381.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33368..33547)
                     /locus_tag="ACHY3M_001441"
     CDS             complement(33368..33547)
                     /locus_tag="ACHY3M_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFS4316382.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33568..34047)
                     /gene="folA"
                     /locus_tag="ACHY3M_001442"
     CDS             complement(33568..34047)
                     /gene="folA"
                     /locus_tag="ACHY3M_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFS4316383.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34239..36101)
                     /gene="kefC"
                     /locus_tag="ACHY3M_001443"
     CDS             complement(34239..36101)
                     /gene="kefC"
                     /locus_tag="ACHY3M_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFS4316384.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36094..36624)
                     /gene="kefF"
                     /locus_tag="ACHY3M_001444"
     CDS             complement(36094..36624)
                     /gene="kefF"
                     /locus_tag="ACHY3M_001444"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFS4316385.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36732..38063)
                     /gene="yaaU"
                     /locus_tag="ACHY3M_001445"
     CDS             complement(36732..38063)
                     /gene="yaaU"
                     /locus_tag="ACHY3M_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4316386.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38122..38409)
                     /gene="fixX"
                     /locus_tag="ACHY3M_001446"
     CDS             complement(38122..38409)
                     /gene="fixX"
                     /locus_tag="ACHY3M_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFS4316387.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38406..39692)
                     /gene="fixC"
                     /locus_tag="ACHY3M_001447"
     CDS             complement(38406..39692)
                     /gene="fixC"
                     /locus_tag="ACHY3M_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4316388.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39743..40684)
                     /gene="fixB"
                     /locus_tag="ACHY3M_001448"
     CDS             complement(39743..40684)
                     /gene="fixB"
                     /locus_tag="ACHY3M_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS4316389.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40699..41469)
                     /gene="fixA"
                     /locus_tag="ACHY3M_001449"
     CDS             complement(40699..41469)
                     /gene="fixA"
                     /locus_tag="ACHY3M_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFS4316390.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41943..43457
                     /gene="caiT"
                     /locus_tag="ACHY3M_001450"
     CDS             41943..43457
                     /gene="caiT"
                     /locus_tag="ACHY3M_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFS4316391.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43488..44630
                     /gene="caiA"
                     /locus_tag="ACHY3M_001451"
     CDS             43488..44630
                     /gene="caiA"
                     /locus_tag="ACHY3M_001451"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFS4316392.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44759..45976
                     /gene="caiB"
                     /locus_tag="ACHY3M_001452"
     CDS             44759..45976
                     /gene="caiB"
                     /locus_tag="ACHY3M_001452"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFS4316393.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46050..47603
                     /gene="caiC"
                     /locus_tag="ACHY3M_001453"
     CDS             46050..47603
                     /gene="caiC"
                     /locus_tag="ACHY3M_001453"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFS4316394.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47712..48497
                     /gene="caiD"
                     /locus_tag="ACHY3M_001454"
     CDS             47712..48497
                     /gene="caiD"
                     /locus_tag="ACHY3M_001454"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFS4316395.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48503..49093
                     /gene="caiE"
                     /locus_tag="ACHY3M_001455"
     CDS             48503..49093
                     /gene="caiE"
                     /locus_tag="ACHY3M_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFS4316396.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49179..49574)
                     /gene="caiF"
                     /locus_tag="ACHY3M_001456"
     CDS             complement(49179..49574)
                     /gene="caiF"
                     /locus_tag="ACHY3M_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFS4316397.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49835..53056)
                     /gene="carB"
                     /locus_tag="ACHY3M_001457"
     CDS             complement(49835..53056)
                     /gene="carB"
                     /locus_tag="ACHY3M_001457"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4316398.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGETGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53074..54222)
                     /gene="carA"
                     /locus_tag="ACHY3M_001458"
     CDS             complement(53074..54222)
                     /gene="carA"
                     /locus_tag="ACHY3M_001458"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFS4316399.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13194 a        14753 c        14257 g        12395 t
ORIGIN      
        1 aatcaatatc aaaaaaatcg caaaacatat aattcaatac aaatcatcag gataggtttt
       61 gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat ttatgctgaa
      121 atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc ttactttaac
      181 caccgcagca caattagcta attttacggg tgcagaactc acgctggcgg gacgttttta
      241 ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgtattcat
      301 ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat
      361 cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca
      421 aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac
      481 aaggacccaa accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga
      541 acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga
      601 gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg gcgattttga
      661 atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg
      721 cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat
      781 tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca gcaccctgga
      841 cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt
      901 tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga
      961 agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat
     1021 gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa
     1081 agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca actcactggc
     1141 ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc
     1201 cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa
     1261 cgtccacacc gccattaatc accaggaaat atggcgcacc agccagttag ttagccagat
     1321 ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcggcgcat tcgcacactc
     1381 ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc
     1441 agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc
     1501 ggtgaaacat cgcatggacg agatggggta taaagaaagt gaatataatt tagacaattt
     1561 gtacgacgcc ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga
     1621 ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt
     1681 cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgtggcga
     1741 agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcaat
     1801 taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca
     1861 cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc gccgcttcca
     1921 cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact
     1981 gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag ctcaacacaa
     2041 cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccgggg
     2101 gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac
     2161 cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggtgcagc cattgataac
     2221 cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg
     2281 tttggctcgg taggcggtcc gaaatgggaa catttaccac cagaccagca accagaacgc
     2341 ggcgcgctgt tgcctttgcg taagcacttc aaattattca gcaacctgcg cccggcaaaa
     2401 ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc taacggcttc
     2461 gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc
     2521 gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc
     2581 gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa agtcacctca
     2641 atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc
     2701 gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc caccatgcag
     2761 ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt
     2821 ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc cgccagcctg
     2881 aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc
     2941 aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc
     3001 ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa
     3061 ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc
     3121 gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa
     3181 aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg
     3241 accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg
     3301 gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac gtctctaccc
     3361 agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta
     3421 aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat caggggatcg
     3481 tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt gtctgcggcg
     3541 actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg
     3601 aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa accatgaaaa
     3661 ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta
     3721 tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa
     3781 tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg
     3841 caaaagccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa ggccgtttgc
     3901 atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaactg
     3961 acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt tcaccgcagg
     4021 tcacctgggg taccaatcca ggccaggtga tttccgtgaa cgacaatatt cccgatccgg
     4081 cttcgtttgc cgatccggtt gaacgcgcgt cggcagaaaa agcgctggcc tatatggggc
     4141 tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgta
     4201 ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg
     4261 cgccaggcgt gcaggcactg gtggttcccg gctctggacc ggtaaaagcc caggcggaag
     4321 cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg cctggctgct
     4381 caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcatccacca
     4441 gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg
     4501 caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac attaaataag
     4561 gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc
     4621 gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtacg
     4681 ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg ccaacagcca
     4741 aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt gctggcacga
     4801 gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggt
     4861 tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac
     4921 cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc gctggtgaaa
     4981 gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa agcgggagag
     5041 aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa cggtctggac
     5101 agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc aaaacaacct
     5161 gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct atcgtccacg
     5221 tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa taaccgcaat
     5281 tacccagtag acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc
     5341 cagaattacc ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc
     5401 aggcattaaa tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc
     5461 gttaaaaagt tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta
     5521 aaacagtact cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac
     5581 atagtagcca gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa
     5641 acccgccagt ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat
     5701 gtacatggtg ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact
     5761 atcctgccag ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac
     5821 agacggaagc ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga
     5881 aaacatcacc gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac
     5941 ccatgccaga gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga
     6001 gttatccgca tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga
     6061 tgccagaagc acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag
     6121 cgcattgccg atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact
     6181 gtcagaacgt tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata
     6241 aaagagtccg atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt
     6301 aggagcctgt agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta
     6361 aacaccgttc atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc
     6421 ggcgagaata aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact
     6481 cagtcacaca tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt
     6541 tcttcaagca tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct
     6601 gtcgcggtaa aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta
     6661 ggaattattg acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct
     6721 tttatgccat ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt
     6781 aaatcgcagg acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat
     6841 atgcgtaccc tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc
     6901 gggcgcggta aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag
     6961 cgggcggaag acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac
     7021 aaagcgactg tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg
     7081 cacatcctgc gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg
     7141 cgccgttccg aaacccatat tgcccggcaa atcttcagtt cgctaacgcg cataaatgag
     7201 gaaaatgggg aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac
     7261 tggcgtttct ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac
     7321 gacgtgatcg cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac
     7381 attgtgtcgc cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta
     7441 ccgttactgc tgggacaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt
     7501 aactttgcca gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat
     7561 caactgaaaa ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt
     7621 aacgtctggg ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt
     7681 ccacagggtg aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttattg
     7741 ttcgacagcc gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg
     7801 ctttctccaa ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg
     7861 gcttatggac tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct
     7921 ggcctggaaa gcctcatcct gaccttttat caggatcaca gtgagcatcg ggtgattgcc
     7981 gggatcatgc agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc
     8041 tacgatcagt ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt
     8101 acgctgccgc tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac
     8161 tgtattccga tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg
     8221 gcgaactggt gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg
     8281 ctgatcattc aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc
     8341 gattttaaat cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc
     8401 actacactaa cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc
     8461 gtcgaacctg atccggataa cgccggcgaa gggatttgag gctccttctc aagtcctttg
     8521 ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac
     8581 agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga
     8641 ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa
     8701 actggtggca ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa
     8761 tagtaaagcc gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac
     8821 cggactattt gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa
     8881 taatgacact ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa
     8941 actgaaaaac ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt
     9001 gatttatcag gatccgcgca ccagtacgcc ggggctgggc ctgctgttgt ggatgcaaaa
     9061 agtctatggc gatgacgccc cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt
     9121 caccaaaggc tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg atctggtact
     9181 gagttacacc acctctccgg cttatcacat tctcgaagag aaaaaagata actacgccgc
     9241 cgcgaacttt agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag
     9301 caagcagccg gagctggcgc aaaaattcct ccagtttatg gtttctccgg cttttcagaa
     9361 tgcgatccca accggtaact ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt
     9421 tgaacaattg accaaaccgg ccaccacgct ggagttcaca ccagctgaag tggctgcaca
     9481 acgtcaggca tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta
     9541 attccaggtg taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc
     9601 ctgtggtgga acgcgccgca gggtaactgg gtggcagtct ggcaggacag ctacctgtgg
     9661 catgtggtgc gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc
     9721 gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg
     9781 cgtctgtgcg caatgacctt gatcctcccg gtgctagttg ctgttttcgg cattcttagc
     9841 gtctatggtc gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc
     9901 ttttcgccct acggcctgca aggcattttg ctggcgcacg tattttttaa tctgccgatg
     9961 gcgagccgct tattactcca ggcactggaa aacattcccg gcgaacaacg tcagcttgcc
    10021 gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg
    10081 cgacaaatcc cgccggttgc cgcgcttatc tttatgctct gtttcgccag cttcgccacc
    10141 gtgctatcgc tgggcggcgg tccgcaggca actactatcg agctggctat ttatcaggcg
    10201 ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt
    10261 tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg ccattgcgcc cggtaccacg
    10321 ctgctgcaag gctggcgcga tccggacgat cgtctgcata gccgcatttg cgacacggtg
    10381 ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg
    10441 gtaaatcgcc agttgccgga agtgctggca caaccggtgc tgtggcaggc gctgtggacc
    10501 tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta
    10561 tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg
    10621 agcggcatgt tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg
    10681 ctcaacaaca ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg
    10741 ttaatggcga tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc
    10801 cgctacagta tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc
    10861 gagctgcgcg ccctgaaacg tccactggcg caggcgctgg cttttgcctg cgtgctgtcg
    10921 attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg atttccgtac cctgccgttt
    10981 tatctctacc agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt
    11041 ctgctactac tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt
    11101 aaaactgact gatatcacct ggctttatca ccatttgccg atgcgtttta gcttaacggt
    11161 agaacgcggc gagcaggtgg caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct
    11221 gaatttgatt gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga
    11281 tcacaccacg acgccgccgt cacgccgccc ggtatcgatg ctgtttcagg agaacaacct
    11341 gttcagccac ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct
    11401 gaacgcggcg cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt
    11461 aatggcgcgg ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg
    11521 ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc
    11581 ggcgttacgt caggagatgc tgacgctggt gagcacgagc tgccagcagc aaaaaatgac
    11641 gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt
    11701 ggtcgccgac gggcgcatcg cctggcaggg taagaccaat gagttgttga gcggtaaggc
    11761 gagtgcttcg gcactattgg ggattacggg ttagtatgca ctgtcggatg cggcgtgaac
    11821 gccttatccg acctacgggt agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc
    11881 atcaggcaac ccgcacaaga ctatctccta acccccaacc actttacgca aaatatcgat
    11941 atacaccggc atcaacgggt ggcgaattaa caccaccagc gccaccacgc cgatggcaga
    12001 aattaacggc gtcagccaca acaaacggcc gcgcgacaaa taatgactta aacgatcggt
    12061 tgctttaccg ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc
    12121 cgtcgccagc agcaaccatt taaactcgcc gctctgcatt ccggctggaa tatcgatcgc
    12181 cgcgcccgcc agaatcccag gcaggaagta aaacggtggc cacagcaggc agccgataat
    12241 attcggcgta atgaattttg ccaccggcag atccagcatt cccgccacca ttggcaccag
    12301 cggacgcgtc gggccaacaa agcgaccgac cagaatggtg aacatgctgt gttgatgcaa
    12361 cgcatgttca gttttatcga gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa
    12421 cggcttttta aaacgccagc ccagccagaa ggagatccag tcgcccagca ggcaaccgac
    12481 gatccctgcc agccaggcgt gccagaaact taactcgccg ctgccaatca gcgctcccag
    12541 ccctgccatc agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa
    12601 ggccaccaac accaccgcca tcaatgaata cacggtagat tgggtaataa agtgttccag
    12661 caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
    12721 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
    12781 cattcacttt ttcttcacaa ccggctcgga actcgctcgg gctggccccg gtgcattttt
    12841 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
    12901 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
    12961 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
    13021 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
    13081 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
    13141 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
    13201 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
    13261 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
    13321 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
    13381 gataacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
    13441 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
    13501 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
    13561 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
    13621 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
    13681 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg
    13741 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
    13801 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
    13861 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
    13921 tatcgcaact ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg
    13981 attgcaattg gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc
    14041 agcggtgaag agatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt
    14101 tgtgatgccc cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa
    14161 gcggcactga aaaccgtgct tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg
    14221 attggcgttg acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg
    14281 gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac
    14341 actgcggttg aagaagcgga agagattacc cgtttgtgcc acgcgccggg caacgttgac
    14401 tactcccgct acattggcgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat
    14461 gtgactcgcc aggacaccgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac
    14521 tgggtgccag ctctgctttc cggtaccacc cgcccgcagg atattcgtcg cggacgttgc
    14581 agcgccgggc ataagtcgct atggcacgaa agctggggtg gcttgccgcc agccagtttc
    14641 tttgatgagc tggacccgat cctcaatcgc catttacctt ccccgctgtt cactgacacc
    14701 tggactgccg atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg
    14761 cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggcgca
    14821 ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt
    14881 gccgacaaac agagcgttgg cgagcgggca gttaaaggta tttgcggtca ggttgatggc
    14941 agcgtggtac ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac
    15001 gcctggtttg gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa
    15061 ctgaaagcgc aaatcaacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg
    15121 gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc
    15181 acaccgaacg ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac
    15241 gctccgctgc tgttcggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg
    15301 gagtgcttta ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg
    15361 cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt
    15421 gttgcctctg accagtgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa
    15481 gtgcacgcag acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg
    15541 caaccgcgca gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg
    15601 gcgatgagcg ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc
    15661 gttgcgactc tataaggaca cgataatgac gatttttgat aattatgaag tgtggtttgt
    15721 cattggcagc cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga
    15781 gcacgttgtt aatgcgctga atacggaagc gaaactgccc tgcaaactag tgttgaaacc
    15841 gctgggcacc acgccggatg aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg
    15901 ttgcgctggt ctggtggtgt ggctgcacac tttctccccg gccaaaatgt ggatcaacgg
    15961 cctgaccatg ctcaacaaac cgttgctgca attccacacc cagtttaacg cggcgctgcc
    16021 gtgggacagt atcgatatgg actttatgaa cctgaaccag actgcacatg gcggtcgcga
    16081 gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc gtggttaccg gtcactggca
    16141 ggataaacaa gcccatgagc gtatcggctc ctggatgcgc caggcggtct ctaaacagga
    16201 tacccgtcat ctgaaagtct gccgttttgg cgataacatg cgtgaagtag cggtcaccga
    16261 tggtgataaa gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg
    16321 cgatctggtg caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga
    16381 gtacgaaagc tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa
    16441 cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt ttcctggaac aaggtggctt
    16501 ccacgcgttc accaccacct ttgaagattt gcacggtctg aaacagcttc caggtctggc
    16561 cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc
    16621 cgccctgctt cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat
    16681 ggaggactac acctatcact tcgagaaagg taatgacctg gtgctcggct cccatatgct
    16741 ggaagtctgc ccgtcgattg ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg
    16801 tattggtggt aaggacaatc ctgcccgact gatcttcaat acccaaaccg gtccagcgat
    16861 tgtcgccagc ctgattgatc tcggcgatcg ttaccgtctg ctggttaact gtatcgacac
    16921 ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca
    16981 accggatctg ccaactgctt ccgaagcgtg gatccttgct ggtggcgcgc accataccgt
    17041 cttcagccat gcgctgaacc tcaacgatat gcgccagttc gccgagatgc acgacattga
    17101 aatcacggtg attgataacg acacccgcct gccagcgttt aaagacgcac tgcgctggaa
    17161 cgaagtgtat tacggatttc gtcgctaagt agccacatca ggtatgtaac gcctgatgcg
    17221 acgctgacgc gtcttatctg gcctacacgc tgcgattttg taggccggat aagcaaagcg
    17281 catccggcat tgttttcccc ctcgcccctt tggggagagg gccggggtga ggggaaacga
    17341 cctctcagga gtcaacatgt tagaagatct caaacgccag gtattagaag ccaacctggc
    17401 gctgccaaaa cacaacctgg tcacgctcac atggggcaac gtcagcgccg ttgatcgcga
    17461 gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac agcgtcatga ccgctgacga
    17521 tatggtcgtg gttagcatcg aaaccggtga agtggttgaa ggtacgaaaa agccctcctc
    17581 cgacacgcca actcaccggc tgctctatca ggcattcccc tccattggcg gcattgtgca
    17641 tacgcactcg cgccacgcca ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg
    17701 caccacccac gccgactatt tctacggcac cattccctgc acccgcaaaa tgaccgacgc
    17761 agaaatcaac ggcgaatatg agtgggaaac cggtaacgtc atcgtagaaa cctttgaaaa
    17821 acagggtatc gatgcagcgc aaatgcccgg cgttctggtc cattcccacg gcccgtttgc
    17881 atggggcaaa aatgccgaag atgcggtgca taacgccatc gtgctggaag aggtcgctta
    17941 tatggggata ttctgccgtc agttagcgcc gcagttaccg gatatgcagc aaacgctgct
    18001 ggataaacac tatctgcgta agcatggcgc gaaggcatat tacgggcagt aatgactgta
    18061 taaaaccaca gccaatcaaa cgaaaccagg ctatactcaa gcctggtttt ttgatggatt
    18121 ttcagcgtgg cgcaggcagg ttttatctta acccgacact ggcgggacac cccgcaaggg
    18181 acagaagtct ccttctggct ggcgacggac aacgggccgt tgcaggttac gcttgcaccg
    18241 caagagtccg tggcgtttat tcccgccgat caggttcccc gcgctcagca tattttgcag
    18301 ggtgaacaag gctttcgcct gacaccgctg gcgttaaagg attttcaccg ccagccggtg
    18361 tatggccttt actgtcgcgc ccatcgccaa ttgatgaatt acgaaaagcg cctgcgtgaa
    18421 ggtggcgtta ccgtctacga ggccgatgtg cgcccgccag aacgctatct gatggagcgg
    18481 tttatcacct caccggtgtg ggtcgagggt gatatgcaca atggcactat cgttaatgcc
    18541 cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt gggtttctat agatattgaa
    18601 accacccgcc acggtgagct gtactgcatc ggcctggaag gctgcgggca gcgcatcgtt
    18661 tatatgctgg ggccggagaa tggcgacgcc tcctcgcttg atttcgaact ggaatacgtc
    18721 gccagccgcc cgccgttgct ggaaaaactc aacgcctggt ttgccaacta cgatcctgat
    18781 gtgatcatcg gttggaacgt ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag
    18841 cgttaccgtc ttccgctgcg tcttgggcgc gataatagcg agctggagtg gcgcgagcac
    18901 ggctttaaaa acggcgtctt ttttgcccag gctaaaggtc ggctaattat cgacggtatc
    18961 gaggcgctga aatccgcgtt ctggaatttc tcttcattct cgctggaaac tgtcgctcag
    19021 gagctattag gcgaaggaaa atctatcgat aacccgtggg atcgaatgga cgaaattgac
    19081 cgccgtttcg ccgaagataa acctgcgctg gcaacttata acctgaaaga ttgcgagctg
    19141 gtgacgcaga tcttccacaa aactgaaatc atgccatttt tactcgaacg agcaacggtg
    19201 aacggcctgc cggtggatcg gcacggcggt tcggtggcag cgtttggtca tctctatttt
    19261 ccgcgaatgc atcgcgctgg ttatgtcgcg cctaatctag gcgaagtgcc gccgcacgcc
    19321 agccctggcg gctacgtgat ggattcacgg ccagggcttt atgattcagt gctggtgctg
    19381 gactataaaa gcctgtaccc gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg
    19441 gttgaaggca tggcgcagcc tgatccagag cacagtaccg aaggttttct cgatgcctgg
    19501 ttctcacggg agaaacattg cctgccggag attgtgacta acatctggca cgggcgcgat
    19561 gaagccaaac gtcagggtaa taaaccactt tcgcaggcgc tgaagatcat tatgaatgcc
    19621 ttttatggcg tgcttggcac caccgcctgc cgctttttcg atccgcgcct ggcatcgtcg
    19681 atcaccatgc gtggtcatca gatcatgcgg caaaccaaag cattgattga agcgcagggc
    19741 tacgacgtta tctacggaga taccgactcg acttttgtct ggctaaaagg tgcacattcg
    19801 gaagaagagg cggcgaaaat cggtcgtgca ctggtgcagc acgttaacgc ctggtgggcg
    19861 gaaacgctgc aaaaacaacg gctgaccagc gcattagaac tggagtatga aactcatttc
    19921 tgccgttttc ttatgccaac cattcgcgga gctgataccg gcagtaaaaa gcgctacgcc
    19981 gggctgattc aggagggcga caagcagcgg atggtgttta aagggctgga aaccgtgcgc
    20041 accgactgga cgccgctggc ccagcagttt cagcaggagc tgtacctgcg tatcttccgc
    20101 aacgagccat atcaggagta cgtccgccaa accatcgaca aactgatggc gggtgaactg
    20161 gatgcgcggc tggtttaccg taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat
    20221 gttccgcctc atgtacgcgc cgctcgcctt gccgatgaag aaaatcaaaa gcgcggtcgc
    20281 cccttgcaat atcagaatcg cggcaccatt aagtacgtat ggaccaccaa cggcccggag
    20341 ccgctggact accaacgttc accactggat tacgaacact atctgacccg ccagctacaa
    20401 cccgtggcgg agggaatact cccttttatt gaggataatt ttgctacact tatgaccggg
    20461 caacttgggc tattttgagc aaaaaaaaga gttcgccaga taccattttg atgcgtgacg
    20521 aatgctttgc catccagtac catagcgccc tttccattcc tggacctgaa taacgccact
    20581 acctcataag cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac
    20641 atatgccttt tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg
    20701 gaaccgttgt cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa
    20761 accgtctgta cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata
    20821 ccattaccag ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct
    20881 tgctgaccta tatcggtact cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag
    20941 ttttccttga tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga
    21001 ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt
    21061 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg catcagctca
    21121 acatcgctca tgatgttggt cgccgccacg cgccgcgcgt tctgctggct gacgaagtgg
    21181 gtttagggaa aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg
    21241 ctgaacgtgt gctgattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc
    21301 tgcgccgttt caacctgcgt tttgcgctgt ttgatgatga gcgttatgcc gaagctcagc
    21361 acgatgctta caacccgttc gacaccgaac agctggtgat ttgctcgctg gattttgccc
    21421 gtcgtagcaa acagcgcctg gaacatctct gtgaagccga atgggacctg ctggtggtcg
    21481 atgaagcgca tcacctggtg tggagcgaag acgcgccgag ccgcgaatat caggctattg
    21541 aacaactggc agagcacgtg ccgggcgttc tgctgctgac cgcaaccccg gaacagttgg
    21601 gaatggaaag ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg
    21661 cgcagttcgt tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc
    21721 tggcaggtaa caaactgagc aatgacgaac tgaacatgct tggcgagatg atcggcgagc
    21781 aggatatcga gccgctgttg caagcagcaa acagcgacag cgaagatgcc cagagcgccc
    21841 gtcaggaact ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta
    21901 acacccgtaa cggtgtgaaa ggattcccga aacgcgagct gcacaccatt aagctgccgc
    21961 taccgacgca gtatcagacg gctattaaag tctccggcat tatgggcgca cgtaaaagtg
    22021 cggaagatcg tgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggtg
    22081 ataacgccac ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc tacctgacca
    22141 gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg
    22201 agcaggtact gcgcgaacgt gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga
    22261 ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga agacaccggc gcacaggtac
    22321 tgctgtgctc agaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtaa
    22381 tgtttgacct gccattcaac ccggatctgc tggagcagcg tattggtcgt ctggatcgta
    22441 tcggtcaggc gcacgatatt cagatccatg tgccttatct ggaaaaaact gctcagtcgg
    22501 tgctggtgcg ctggtatcac gaaggtctgg atgcattcga gcacacctgc ccgaccggac
    22561 gcactattta cgatagcgtc tacaacgatc tgattaacta tctggcttca ccggatcaaa
    22621 ccgaaggctt tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac
    22681 aactggaaca ggggcgtgat cgcctgctgg aaatccactc caacggtggc gaaaaagccc
    22741 aggcactggc agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca
    22801 tgaacctgtt cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc
    22861 tgaccccgtc cgatcatatg ctggtgccgg acttccctgg cctgtcggaa gatggcatca
    22921 ccatcacctt tgatcgtgaa gtggcgctgg tgcgtgaaga tgctcagttt attacctggg
    22981 aacatccgct gatccgcaac ggtctggatc tgattctttc tggcgatacc ggtagcagca
    23041 cgatttcact gttaaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt
    23101 acgtggtcga agcccaggcg ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc
    23161 cggtacgtat gctgctggat aaaaacggca acaacctggc ggcccaggtg gagtttgaaa
    23221 cctttaaccg ccagcttaac gcggttaacc gtcacaccgg cagcaaactg gttaacgccg
    23281 tgcagcagga tgttcacgct atccttcagc tgggtgaagc gcagatcgag aaatctgccc
    23341 gtgcattgat tgatgccgca cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc
    23401 gtctggaagc gctgcgcgca gtgaacccga acattcgtga cgacgaactg accgccattg
    23461 agagcaaccg tcagcaggta atggaaagcc tggatcaggc aggctggcgt ctggatgccc
    23521 tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa atggggatgg aaaactacaa
    23581 tccaccgcag gaaccctggt tggttatcct gtatcaggat gaccacatta tggtggttaa
    23641 caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag atagcgtgat
    23701 gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac
    23761 cagcggcgtg attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt
    23821 ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc tgggggcatc catcccccgc
    23881 agaaggtctg gtggatctgc cgctgatttg cgactggcca aaccgcccga aacagaaagt
    23941 ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa gtggtggagt atgtggcgga
    24001 taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca
    24061 tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag
    24121 agcgatggca ccacgtttgt tgctacatgc agagatgttg acgattaccc atccggcgta
    24181 tggcaatagt atgacgttta aagcgccagc ggatttttaa tcgtcacgct ggcgcagttc
    24241 ggtaggcgga taaggcgttc acgccgcatc cggcaattgt gcgccgattg cctgatgcga
    24301 cgcgggccgc gtcttatcag gcctacagtt taaaacttat ttaaaccctt tctgctgctt
    24361 tatcagctca tatgcctgct gaatttcctg cgctttctgc ttcgccatct ccatcatctc
    24421 aggcggcaaa cctttcgcca ccagcttatc gggatggtgt tcactcatca gcttacggta
    24481 ggcacgtttg atggtggtcg catcatccgt cggcttcacg cccagcacat tacaggcatc
    24541 ttccagcgtc gggccacgct gcgcttgctg ccagttacca ccgccagatt gctgctgata
    24601 accgccgcca aactgtgcac cgccctgcat catgcgcaaa aactggtcaa actgggcgcg
    24661 ggagatccct aattcttcag caatgacata cagcaccgcc cgttcattcg ggtgcagtga
    24721 accatcagca aacgccgcct gaatctggat ctccagaaac atacgaatta agtcaaaacg
    24781 accaaagcag acactgcgaa actggcgcat cttttcgcgc agcgggtaat tatctgattt
    24841 tcccactcgg aacgcgtttt gcgccgcagt acgggaagcg ccatgaaggt tcattcggtc
    24901 catcaactgg ctggcgatat gaatatcagc ctccgtgacg cgacctttgg atttggttaa
    24961 atgccccatc acttcaaaag tggtggcaaa aaacagcgcc tgacgctcac gctggttggc
    25021 gaaccacgcc attttacggc tacgggcttt atcaaacata tggccaatta acaggcctaa
    25081 cactacgccc caaaagccgc cgcccatcag taaggccacg gccacgccaa tgatttttcc
    25141 ccaatactgc atatattccc caaatcgaca cacggatatc agggctatct cccacaatat
    25201 aaaggtgctt ttaccgtttt ccggctgcgg tcaattgtgg gacatcgcct ataatttgca
    25261 ttatcatacc tgtcattcaa tgccgtgcct aacaccactg acgctattcg gacaggatta
    25321 acactagcgt agagatgacg agtacgttag tctctgaccg tttgtcacgc gcaacgttac
    25381 cgatgatgga acaataaaat caacgtatga aaaaacgtat ccccactctc ctggccacca
    25441 tgattgccac cgccctttat agtcaacagg gactggcagc cgacctcgcc tcacagtgca
    25501 tgttgggcgt gccaagctat gaccgtcctc tggtacaggg cgataccaat gacttacccg
    25561 tgactatcaa tgctgaccac gcgaaagggg actacccgga tgacgccgtg tttactggca
    25621 gcgtggatat catgcagggt aacagccgtc tgcaggccga cgaagtgcag ctccatcaaa
    25681 aagaggcacc aggacaaccg gagccggtac gtaccgttga tgcgctcggt aatgtccatt
    25741 acgacgataa ccaggtgatc ctcaaagggc cgaaaggctg ggcgaatctg aacaccaaag
    25801 ataccaacgt ctgggaaggt gattaccaga tggtgggtcg ccagggtcgc ggtaaagcgg
    25861 acctgatgaa acaacgtggc gaaaaccgct ataccattct ggataacggt agctttacct
    25921 cctgtctgcc gggttctgac acctggagcg tggtaggtag cgaaattatt catgaccgcg
    25981 aagaacaagt tgcggagatc tggaacgccc gctttaaggt gggtccggta ccgatctttt
    26041 atagccccta tttgcagttg ccggtgggtg acaaacgtcg ctctggtttc ttgatcccga
    26101 acgccaagta caccaccacc aactactttg agttctacct gccatattac cggaacatcg
    26161 cgccaaatat ggatgccacc atcacgccgc attatatgca tcgtcgtggc aacatcatgt
    26221 gggagaacga attccgctac ctctcccagg cgggcgctgg cttgatggaa ctggactatc
    26281 tgccttcaga taaagtctat gaagatgaac acccgaacga tgacagttca cgtcgttggt
    26341 tattctactg gaaccactcc ggggtcatgg atcaggtgtg gcgtttcaac gtcgactaca
    26401 ccaaggtcag cgatcctagc tacttcaatg atttcgataa caagtacggt tccagtactg
    26461 acggctacgc aacgcaaaaa ttcagcgttg gctatgcggt gcaaaacttc aatgccaccg
    26521 tttcaaccaa gcagttccag gttttcagcg aacagaacac cagtagctac tcggcagagc
    26581 cgcagttaga cgttaattac taccagaatg atgttggtcc gtttgatacg cgtatttacg
    26641 gccaggcagt gcactttgtt aacaccagag acgacatgcc tgaagcaacc cgtgttcacc
    26701 tggaaccgac catcaatttg ccgctctcta ataactgggg cagcatcaat accgaagcga
    26761 agttgctggc aacccattat cagcaaacca atcttgactg gtataactcc agaaacacga
    26821 ccaagctgga cgaatccgtt aaccgcgtaa tgccgcaatt caaagttgac ggcaaaatgg
    26881 tctttgaacg cgatatggaa atgctggctc cgggttatac ccaaacgctg gaaccgcgcg
    26941 cgcagtattt gtacgtgccg tatcgcgatc agagcgacat ctataactac gactcgtctc
    27001 tgctgcaatc tgactactct ggcctgttcc gggaccggac ttacggcggt cttgaccgta
    27061 ttgcctccgc taaccaggtg acgaccggtg tcacatctcg catatatgat gatgctgccg
    27121 ttgaacgttt taatatttcc gttggtcaaa tctactattt cacggagtct cgcactggcg
    27181 atgacaacat aacatgggag aatgacgaca aaacgggttc actggtgtgg gcaggcgata
    27241 cttactggcg tatctccgag cgttggggat tgcgtggcgg gattcagtac gatacacgtc
    27301 tggataacgt agcgaccagt aactccagca ttgaataccg tcgggatgaa gaccgtctgg
    27361 tacagctgaa ttaccgttac gccagcccgg aatatattca ggctacgctg cctaagtact
    27421 attccactgc tgagcaatat aagaatggta tttcgcaggt aggtgctgtc gccagctggc
    27481 caattgccga tcgttggtcc attgttgggg cctactacta cgacaccaat gcgaacaagc
    27541 aagccgactc tatgttaggt gtgcaataca gctcctgctg ctatgcaatt cgcgtcggtt
    27601 acgagcggaa gctgaacggt tgggataacg ataaacaaca tgcggtatat gacaacgcaa
    27661 tcggctttaa catcgaactt cgcggcctga gctccaacta cggtctgggt acgcaagaga
    27721 tgctgcgttc gaacattctg ccgtatcaaa acactttgtg atttgttgat ttaccacgta
    27781 atccgcagtg cggttaattg aaatggaaaa agtatgaaga actggaaaac gctgcttctc
    27841 ggtatcgcca tgatcgcgaa taccagtttc gctgcccccc aggtagtcga taaagtcgca
    27901 gccgtcgtca ataacggcgt cgtgctggaa agcgacgttg atggattaat gcagtcggta
    27961 aaactgaacg ctgctcaggc aaggcagcaa cttcctgatg acgcgacgct gcgccaccaa
    28021 atcatggaac gtttgatcat ggatcaaatc atcctgcaga tggggcagaa aatgggagtg
    28081 aaaatctccg atgagcagct ggatcaggcg attgctaaca tcgcgaaaca gaacaacatg
    28141 acgctggatc agatgcgcag ccgtctggct tacgatggac tgaactacaa cacctatcgt
    28201 aaccagatcc gcaaagagat gattatctct gaagtgcgta acaacgaggt gcgtcgtcgc
    28261 atcaccatcc tgccgcagga agtcgaatcc ctggcgcagc aggtgggtaa ccaaaacgac
    28321 gccagcactg agctgaacct gagccacatc ctgatcccgc tgccggaaaa cccgacctct
    28381 gatcaggtga acgaagcgga aagccaggcg cgcgccattg tcgatcaggc gcgtaacggc
    28441 gctgatttcg gtaagctggc gattgctcat tctgccgacc agcaggcgct gaacggcggc
    28501 cagatgggct ggggccgtat tcaggagttg cccgggatct tcgcccaggc attaagcacc
    28561 gcgaagaaag gcgacattgt tggcccgatt cgttccggcg ttggcttcca tattctgaaa
    28621 gttaacgacc tgcgcggcga aagcaaaaat atctcggtga ccgaagttca tgctcgccat
    28681 attctgctga aaccgtcgcc gatcatgact gacgaacagg cccgtgtgaa actggaacag
    28741 attgctgctg atatcaagag tggtaaaacg acttttgctg ccgcagcgaa agagttctct
    28801 caggatccag gctctgctaa ccagggcggt gatctcggct gggctacacc agatattttc
    28861 gatccggcct tccgtgacgc cctgactcgc ctgaacaaag gtcaaatgag tgcaccggtt
    28921 cactcttcat tcggctggca tttaatcgaa ctgctggata cccgtaatgt cgataaaacc
    28981 gacgctgcgc agaaagatcg tgcataccgc atgctgatga accgtaagtt ctcggaagaa
    29041 gcagcaagct ggatgcagga acaacgtgcc agcgcctacg ttaaaatcct gagcaactaa
    29101 tggttaaaac ccaacgtgtt gtgatcactc ccggcgagcc cgccgggatt ggcccggact
    29161 tagttgtcca gcttgcacag cgtgagtggc cggtcgaact ggttgtttgt gccgatgcca
    29221 ctctccttac cgaccgggca gcgatgctcg gtttgccgct caccctccgc tcttattccc
    29281 ccaactcccc tgcacaaccg caaactacgg gcacattaac gctacttcct gtcgcgctac
    29341 gtgaatctgt cactgcgggg cagttagcga ttgaaaatgg acattacgtg gtggagacgc
    29401 tggcgcgagc gtgtgatggc tgtctgaacg gtgaatttgc tgcgctgatc acaggccccg
    29461 tgcataaagg cgtcattaac gacgcaggca ttccgtttac cggtcatacc gagtttttcg
    29521 aagagcgttc gcaggcgaaa aaagtggtga tgatgctggc gaccgaagaa cttcgcgtgg
    29581 cgctggcaac gacgcattta ccgctgcgcg atatcgcaga tgctatcacc cctgcacttt
    29641 tgcacgaagt gattgctatt ttgcatcacg atttgcgtac aaaatttggt attgccgaac
    29701 cgcgcattct ggtctgcggg ctgaatccgc acgcgggcga aggcggtcat atgggtacgg
    29761 aagagataga caccattatt ccggtgctcg acgagctgcg ggcgcagggg atgaaactca
    29821 acgggccgct gcctgccgat accctgtttc agccgaaata tctcgataac gccgacgccg
    29881 tgctggcgat gtaccacgat cagggtcttc ccgtgctaaa ataccagggc ttcgggcgcg
    29941 gtgtgaacat tacgctgggc ctgcccttta ttcgcacatc agtggaccac ggcaccgcgc
    30001 ttgaactggc gggacgtggc gaagccgatg tcggcagttt tattacggcg cttaatctcg
    30061 ccatcaaaat gattgttaac acccaatgaa taatcgagtc caccagggcc acttagcccg
    30121 taaacgcttc gggcaaaact ttctcaacga tcagttcgtg atcgacagca ttgtttctgc
    30181 cattaacccg caaaagggcc aggcaatggt cgaaatcggc cccggtctgg cggcattgac
    30241 cgagccggtc ggcgaacgtc tggaccagct gacggtcatc gaacttgacc gcgatctggc
    30301 ggcacgtctg caaacgcatc cattcttagg cccgaaactg acgatttatc agcaggatgc
    30361 gatgaccttt aactttggcg aactggccga gaaaatgggt cagccgctgc gtgttttcgg
    30421 caacctgcct tataacatct ccacgccgtt gatgttccat ctgtttagct atactgatgc
    30481 cattgccgac atgcacttta tgttgcaaaa agaggtggtg aatcgtctgg ttgcaggacc
    30541 gaacagcaaa gcgtatggtc gattaagcgt catggcgcaa tactattgca acgtgatccc
    30601 ggtactggaa gttccgccgt cagcctttac accaccaccc aaagtggatt ccgccgtcgt
    30661 gcgcctggtt cctcatgcaa cgatgcctca cccggttaaa gatgttcgtg tgttgagccg
    30721 catcaccacc gaagccttta accagcgtcg taaaaccatt cgtaacagcc tcggcaacct
    30781 gtttagcgtc gaggtgttaa cgggaatggg gatcgacccg gcgatgcgag cggaaaatat
    30841 ctctgtcgcg caatattgcc agatggcgaa ctatctggcg gagaacgcgc ctttgcagga
    30901 gagttaacga tgatcaattc gccccgagtg tgtattcagg ttcaaagcgt ctacattgag
    30961 gcacaatctt cacctgataa tgaacgttac gtttttgctt ataccgtaac catacgcaat
    31021 ctggggcgaa cgccagtgca gttgttgggg cgttactggc tgatcaccaa tggcaatggc
    31081 cgtgaaaccg aagtccaggg cgaaggagtg gttggcgtcc agccacttat cgcgcctggc
    31141 gaagagtacc agtacaccag cggtgcaatc attgaaaccc cgctgggcac catgcagggt
    31201 cactacgaaa tgatcgatga aaatggcgtc cctttcagca tcgacattcc cgtattccga
    31261 ctcgccgttc ccacactcat tcattaaaag aatatggcga cataccttat tggcgacgtt
    31321 catggttgtt acgatgaact gttcgcattg ctgcataaag tagaatttac ccctgggaaa
    31381 gataccctct ggctgacggg cgatctggtc gcgcgcgggc cgggttcgct ggatgttctg
    31441 cgctatgtga aatccttagg cgacagcgta cgtctggtgc tgggcaatca cgatctgcat
    31501 ctgctggcgg tatttgccgg gatcagccgc aataaaccga aagatcgcct gacaccgctg
    31561 ctggaagcgc cggatgccga cgagctgctt aactggctgc gtcgccagcc tttgctgcaa
    31621 atcgacgaag agaaaaagtt ggtgatggcc cacgcaggga tcacgccgca gtgggatcta
    31681 cagaccgcca aagagtgcgc gcgcgatgta gaagcggtgc tatcgagtga ctcctatccc
    31741 ttctttctcg acgctatgta cggcgatatg ccaaataact ggtcaccgga attgcggggg
    31801 ctgggaagac tgcgttttat caccaacgcc tttacccgta tgcgtttttg ctttccaaac
    31861 ggtcaactgg atatgtacag caaagaatcg ccggaagaag cccctgcccc actgaaaccg
    31921 tggtttgcga ttcctggccc tgtcgctgaa gaatacagca tcgcctttgg tcactgggca
    31981 tcgctggaag gcaaaggtac gccggaaggt atttacgcgc tggataccgg ctgctgctgg
    32041 ggtgggtcat taacctgcct gcgctgggaa gataagcagt attttgtcca gccgtcgaac
    32101 cggcataagg atttgggtga gggagaggcg gtcgcgtctt aacgaattac cattcaggga
    32161 ggaataatgg ctgactattt tgaaatacgt gctgattaca atcagcacac catcacgatt
    32221 tatcaggctt acaatgatgc aatcgctgac gtcgccgtac gtgacgggcg atttggcgca
    32281 cctttttcat tcaaccgcat gacctggatt aaaccctctt ttatgtggat gatggagcgt
    32341 agcaactggg gactgaaaaa agatcaacaa cacattctcg caatccgaat caaacgcacc
    32401 ttctttgata cattgctgga acaagcggtg ctaaccacgc cggaagccca tgtgtatcca
    32461 catgcaggaa tttgggaaac gttatttgca caagcaaatg tgtatgttca gtgggaccca
    32521 gaacgcagca taaatggtaa aaaactggag caccgatcat tacagcttgg tatttctcgt
    32581 aacttgatca gtcaatttaa cgaagacgcg attgttgcta ttgatgatct gacgccgctg
    32641 gtgcgaaagt gtcataacct tttgataaac ggcaaaacaa cacaggcgaa atccttcctg
    32701 ccgccagaaa aaatttaccc agttagtgca gcagcgagaa aggctttggg aatgaaaaat
    32761 tcctgagaca aatacgcacc tattaagcgg aaaatatcta agtgcgacaa atcaacaccg
    32821 ggcttaatac cagagaaatt attctggaaa tgattgacga agcagatgcc cccccgtttg
    32881 ctgagcagcg gacctggcat cgcaggcgaa tcgcttttct atgtgattgc atcagcgttg
    32941 ctgtcgacta cggcttcttc tgttctgtct ctcagcagtc aataatgaac ataaccaaga
    33001 tggctgaaga tgtatggata acagaaagta aaacgaccca actcattgat atttggcaac
    33061 aacttaaaca gctttatccg ccagaaacta ttccctacgc ccagaagtta cccactaagg
    33121 aattgcttga actgctactt tctagtcctg acgatcttcg aataattgaa attcctgaca
    33181 tcatcggtga tgtttttgat gaccaaacct ggattcgatt attcaaaaaa cactttgctg
    33241 atgttattca gctacgatgt gatgtgtaac aatccaaaat atatcgccag acgatagacc
    33301 aaaaagaaag tatttagcag atactcaaca ttctgacttc tgtacagaaa tagcagagct
    33361 taaaaactta aaacttccgg tactcatcag agaaacatcc agtttcttct gcaaattcat
    33421 ttaatgccga gatggcatcc tcgtttactt cattccactg tagagtggct tgaaggttta
    33481 gacctacgca atgagcgcga ctcacaaaat cgcgctcact ggtaaaatca aacgtcttat
    33541 cgttcaattt gtagtcctgg caaaaaatta ccgccgctcc agaatctcaa agcaatagct
    33601 gtgagagttc tgcgcatcag catcgtggaa ttcgctgaat accgattccc agtcatccgg
    33661 ctcgtaatcc gggaaatggg tgtcgccttc cacttctgcg tcgatatgcg tcagatacag
    33721 tttctgcgct tttggcagga actgctcata aacgcgaccg ccgccaatca ccatgatttc
    33781 tggtacgtca ccacacgccg cgatggcttc atccaccgac ttcacccacg ttacgcgatc
    33841 gtccgtaccc ggttgactgc tgaggataat atttttgcgt cctggcaacg gacgaccgat
    33901 tgattcccag gtatggcggc ccataatcac gggtttattt aaggtgttgc gtttaaacca
    33961 ggcgagatcg gcaggcaggt tccacggcat ggcgttttcc atgccgataa cgcgatctac
    34021 cgctaacgcc gcaatcagac tgatcattga gatttcccga taaaaaaaat tgtcgccact
    34081 atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga aaattttata
    34141 ttctgctggc gagtccacgc tctctccctg gactcgccgc attacaatga aacaaaaaca
    34201 aacagttagc tgtaaagtgt gatttacgtc actctttatt aggatgaggg tttcgtttcc
    34261 ggttcatccg ccatgttgcc ggtatgttta ccttcttcgg ttccctgcca gccatgtcgt
    34321 tgaattaatg acagatgttc gcggtcctcg gtaatgatct cacttaacat cgcgctggtg
    34381 cgtttataga ccgccgcgcg ggctttggtg tcgttctcaa ccattgccat ctcttccacc
    34441 atctgaatat taaagcggcg gaacacatcg gcacgttctc gcgcttcata cggccccaga
    34501 cctaaacttt ccagtgccag acgcccggtt ttcagcgcac cttcgaaggt ttcacgctcc
    34561 ggcttttcaa cgcctgcctg acgcaaacga atgtagtggt cgacatcgcg ggcgcgggca
    34621 ataatctgca aatgcgggaa atgttctttc accatctccg tcagttgcag gttggtttgc
    34681 ggatcatcaa tagcattaat cagcacttcc gctttcgccg ctccggcaga ttccagtaaa
    34741 tccatccgcg ttgcatcgcc ataaaacact ttcataccaa atttacgcag cgtttcgata
    34801 tggtccggat cgtgatcgag caccaccatt ttcaccccgc tggagagcag taaacgtccg
    34861 gtaatctgcc caaaacgacc gaatccggcg ataatcacgc gcggctgttc ttcgtcgatc
    34921 tcatcggctt cacgcgcttc ctcagtagaa gattgctcaa ggcgattgag gatcaccagc
    34981 agaatcggcg ttgctgccat cgacagcgcc accgccaggg tcagcgattt cgcccactcc
    35041 ggctccagca cattcgccat ctgcgccgcg ccaaatacca caaaggcaaa ctcactgccc
    35101 tgccctaaca acaccgcaaa ccaacgacgc tgtttatttg gcacttgcaa cggtcgggca
    35161 atcagccaca gcatggcgat tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc
    35221 aatgggtttt caagcagcgt gccaaagtct atcgacatgc caacaccgat gaaaaacagc
    35281 cccaacagca aacctttaaa tggttcgata tcgctctcca gcgcatgacg gtattccgag
    35341 cttgccagca gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc
    35401 agcagcaaac caaagccaaa cacgaggaat aacgccacgg cactaaacac ttcccgcaag
    35461 ccagagcggg ctacaaaacg cagcgccgga cgcgtgacat agcgccccag caataccacc
    35521 agcaccagcg cacccgccac ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag
    35581 gcactgctcg ttgccagtag cggaatcatc gccaccagcg ggatcgccgc gatatcctgg
    35641 aacagcagca ccgcaaaggc actgcgaccc atttgcgtca ccatcagatt gcgttcattc
    35701 atcgcctgca tggcaatcgc cgtagaggag agcgccagcg tcatgccgat caattccgcg
    35761 acctgccagc gcagcccaag taacatgcag aacagcccca gcaggccgcc gcaaatcacc
    35821 atctgcaatg cgccaccgcc gaacactgcc gcacgcagct tccacagcct ttgtggatcg
    35881 agttcgaggc cgataataaa cagcatcagc accaccccaa tctcggcaaa gtgcagaata
    35941 gattcggcat cggtcaccag tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg
    36001 tagccaagta ccgatcccag accaagacgt accgcaatgg gtacaatcag cgctgccgaa
    36061 ccgagataaa tcagcgcctg aatcagcgta tggctatcca tgatgggcct cctgccattc
    36121 cagcagacgt tgcttatagt gacgcgcctg cccttcgagg gtttcgtcgt cacaaataaa
    36181 ggtgcagtgc atggcaaacg gtggcagcca gttcagtccg cagtagattg ccgtcgcctg
    36241 tagcggctgc gacagcacat caaagcccgg atgcgcacca atttcaaaat ggctttcccc
    36301 gccgccggtc gtcaccgccc acagcaaatg tttgccatgc agcgccgtgc cgccatgacc
    36361 gtaagcccag ccgtgggaga aaactttatc gatccaaagt ttgaggagcg gaggaatgct
    36421 gtaccactgc atcgggtgct gccagacgat cagatcggcg cgagacagcg cctcctgctc
    36481 ggcggcaata tcgatattga agtcaggata gagttgataa agagagcgaa tttcgacgcc
    36541 ttccagcgtc cttgcctgtt caagcatccg tttattcgca tgggaatgat gcggatacgg
    36601 atgcgcataa attataagaa tcataggtag cccgcgtgat tacgcctttg ttttcccaca
    36661 aagtgtagtc atatattcac gcggcttata gtcaataata ttgattcaga aaagagagaa
    36721 atctgaacaa tttaccccat tctctgcccg cggatcgtca tattgctggt ttgcgccagt
    36781 gacatccctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata
    36841 cccgccccca ttagcatcgt gttactgata ccgtaattat tgataaagat cggcagtgcc
    36901 caggtcgaaa caatggtgcc aatacggctt aaggacataa tcacgcccac ggcagaggcg
    36961 cggatatctg tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca
    37021 gagaaaaagg catacaccgc aaaggccatc actaccagcc agatccccat atccgggatc
    37081 agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc
    37141 cgtccggcag tgtttaacca cagcatcggc ggaatacagc cgagcataaa gaacaggcta
    37201 atcaccacgt tccccagcgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt
    37261 tgcgggccaa aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca gatggcggca
    37321 acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc
    37381 ggctgctctt catcgaaagc caccggttcg ccaaacagtt ttatcatcat ctcttcgcac
    37441 tcttttactc gccctttgcg taataaccag cggggagatt caggcagttc gaatcgacca
    37501 atcaaaatca acaaacaggg gatcgccgcg ctacccagca tccagcgcca gccgccttcc
    37561 acatcataaa gccagtagcc gaccagatcg gcgcaggtcg cgccgacata ccacatggcg
    37621 gcgataaagc tgatggaaaa cgcccgctga cgggtactgg agaactcggt gatcattgag
    37681 gtggcgatgg gataatctgc accgatgaca atgccgataa gtacccgcat caccaacagt
    37741 tcgacggggg atgaaacaaa catcgtcgcc accgatatca cgccgatggc gatgatatca
    37801 atgaggaaca ttttgcgccg tccgacttta tcggaaatat agccaaacag cgatgtgcca
    37861 acgaacagcc cggcgagcgt tcccgcgccc aacaagccaa tccagtcagc gtccagtttt
    37921 agcgccggag tcagttgctc cagcgccacg ccaatcatta ccagtacata accatccaga
    37981 aacggaccac cgcttcccca cagcaaaatg cggcggtgaa ttgaggagaa tttgagatcg
    38041 tcaaagtttc tggacggttg catgggttat tcctgttttt ttgcaatgag agtcagtacg
    38101 gcgttacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg
    38161 gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag
    38221 acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg
    38281 gcacgctttc accagcagct ccagggcctg tttatcggca tcggccttaa caacaatgtg
    38341 cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg
    38401 agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact
    38461 ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg
    38521 aacaggtcac gcgccacgcc caccgccagt tccgggtagc cgctaaacat gcgtgggtta
    38581 tcaaggaagg ccggtagttt ctggtacata cgcatatcgc gcagcgggcc actctcaaga
    38641 tgctgacgat attccgcgag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc
    38701 acggtttttg ctgcagcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa
    38761 ccgaggttca tacacattcc ggcggcatca ccggcaatca atacgccgtc accaaccaac
    38821 tccggcagca tgttgatgcc tgcttccggc actacgtgag cggaatattc caccagcttg
    38881 ccgcccgcga tcagcggtgc cactgccgga tgctgtttga aatcttccag catttgcggc
    38941 accgattttt ttgcgtcatg cagatgatgc agaccacaaa ccagccccag cgacagggta
    39001 ttttcattgg tataaaggaa gccgccgccc atcaggccat cggtgggtga tcccgcaaac
    39061 agacaagccg cgccctgatt accctgcaac tgaaaacggt cttcgattac cgacttcggt
    39121 aactcgatca gttccttcac gccaaccgcc acatccgtcg gtttaacgcg ttttgccatc
    39181 cccagctttt cggcaaggat ggagttcact ccatcagcaa ggatcaccgt tttcgcttca
    39241 atcacatcgc catcggcttc tacaccgacg actttgccat cgcgctgtac gaggttatcg
    39301 acgcggatcc cggtaattaa ctgcgcgccc gcttcttcgg cctgctccat cagccaggca
    39361 tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gcgaggtttc gtcaccattg
    39421 cagtagtcca tagtcatcgc tgacttttcc gtcataaacg cgagtttttc atgggtgatc
    39481 aggcgttcta cgggggcgga gtcggcgaaa ccaggaataa tgtgttccag gctgtgggca
    39541 tagagacgcc cgccggtgac gttcttggca cctgcggaat tgccacgctc gataactaac
    39601 acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag cccagcaccg
    39661 acgatgatgg cgtcaaagat atcttcggac ataagagcta cctgtcagcg gcagggccaa
    39721 aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagcgcc ggaaggatct
    39781 tcacggcgtc accaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt
    39841 tattgatggc gaaaatggtt tgcgatgcat tagcaccaac catgtgctgg atctgcccgg
    39901 agatccccac cgccaggtac agttcaggtt tcagcatcag gttggagata ccgacatagc
    39961 gttcgtgctc catccatttt tcgttttccg ccaccggacg agaacaggcc aactccgcac
    40021 ctatcgcctt gcaaagctgt tctgccagcg caatgttctc tttgctgcca ataccgcgac
    40081 cgacgctgac caccagacgg gctttgtcga gatcgacgct gttgctctgg cgcgcctggg
    40141 ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag
    40201 tgcgtgacgc gtctggctga gccgcatcga acgtgccgct gctgatggtc agtaccgcat
    40261 acggcgtggc aatgcgttct tcgccaatcg ccagaccacc gtaaaccatg tgtttcactg
    40321 tcgctttacc gtcctgtacg ctgacggtgc tggcatcgtt agacaccgcc gctttaaggc
    40381 gataacccag ttttgccgcc agtaatttgc cgcgacgggt gtttggcagc agcaccaggc
    40441 cgtctgcgcc gtgctggcga atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc
    40501 gatcgtccgg tttgccgttt aatttccaga catgattagc gccgagctgg attgcctgta
    40561 cgccgtcggc atcattgagg acaaaggtgt tgatttgatt agctaaagcc tgcgcaccgt
    40621 tcatcagttc cggcagacga gaaggggtat cgctgaatac ccagacttga gaaaacgtgt
    40681 tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg cggcgatctg
    40741 ttcttcgccg tcgccttcaa tcacgatgcg ctgacgttcg cgctgtttcg gcgcagcaac
    40801 ctgttgttca gaccaggcgt ccacggcgtt aaaaccgata tccgccgccg accatacctg
    40861 gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat
    40921 atcagtggaa acagcaacaa ccgcaggcag cggaatgctt aaggtttcgg tttcatcttc
    40981 cagttcgcgc tcaacggtga gggtatctgc agtcagggag ataattttgc tgacgccgtt
    41041 aactgccgga atattgagga tttcgcccac cagcagacca acctgctggg cataaaggtc
    41101 ggaagaacca tcgccacaga ggatcaaatc aaagcctgct ttctgggcgg ctgcagccag
    41161 tgcgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag
    41221 ttcatccggg ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc
    41281 gcccacactt aaggccgtca cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc
    41341 ttcaatagcg ttcagatcgt attggcttat tttggcatcg gctttgctga agtctaatga
    41401 accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat
    41461 aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat
    41521 tacagatatc acacccttta atttggaaat aaaactttaa tcaccaatat tgaaaatgtc
    41581 acacgcagat aaatccaact ttcaatattg ttaagttcct caccaatatt gaaaacatcg
    41641 cgtagcaaaa agaaattttc aatattgctt tatggatcac caatattgaa agtcagatgc
    41701 tattttttta ctcacaacac aacataacaa actgattaat aatcaaaatt aacgagaaaa
    41761 gcgccctcta ttcacccgaa acaaaaatgt gataacaatc acagaatgca gcttattgaa
    41821 tacccattat gagttagcca ttaacgcgtc cacgaggtta ataataatta tattaaatgt
    41881 taacaaaaat aaaacaaacg ggaacgcaaa ataaatattc gttttcacag tggaattaac
    41941 tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa
    42001 taatcgtcgg catactttgt tggcttacag tcagagatct ggatgcagcg aatgtcgtta
    42061 ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg
    42121 tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag
    42181 gtaacgaacc acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta
    42241 cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc
    42301 cgtttggctt agaaccgaac tcgacagggg cgaaagagtt ggggctggct tacagcttgt
    42361 tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact
    42421 tcttctttgt ccgcaaaatg gaagtgattc gccccagctc gacactggtg ccgctggtag
    42481 gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct
    42541 tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc
    42601 aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga
    42661 ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg
    42721 acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca
    42781 gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca
    42841 tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct
    42901 actgggcatg gtgggtgatt tatgctatcc agatgagtat cttcctcgcc cgcatctccc
    42961 gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct
    43021 ggatcctgtg gactgtactc ggtagtaaca ctctgctgtt gatagataaa aacatcatca
    43081 acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcattgaa acctgggccg
    43141 ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg
    43201 ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg
    43261 atggtgaaga accacctctg ctggtgcgta tcggttggtc aattctggtt ggcattatcg
    43321 gtattgttct gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag
    43381 gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac
    43441 agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt
    43501 taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact
    43561 gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag
    43621 cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg
    43681 cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct
    43741 atgtgctgta ccagttgccg ggcgggttca acaccttcct gcgcgaaggc acacaagagc
    43801 agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta
    43861 ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg
    43921 gtaagattta tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca
    43981 tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg
    44041 ttgatatgag caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg
    44101 atagctgctg tgaaatcacc tttgacgacg tggaactgga cgagaaagac atgttcggtc
    44161 gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag
    44221 ccctcaccaa ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaatc
    44281 agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc
    44341 acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag
    44401 acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg
    44461 catttgaagt tgtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc
    44521 accgcatcag ccgcttctgg cgtgatctgc gtgtagaccg cgtctccggg ggatctgacg
    44581 aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc
    44641 tgcctgatgc gacgctaatg cgtcttatca ggcctacggg agcacgaatg taggccggat
    44701 aaagcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
    44761 ggatcatcta cccatgccga aattcgggcc gttggccgga ttgcgcgttg tcttctccgg
    44821 tatcgaaatc gccgggccgt tcgccgggca aatgttcgca gaatggggtg cggaagttat
    44881 ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
    44941 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc
    45001 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt
    45061 tgcccgtcgt ggcattaccg atgaagtact gtggcagcac aacccgaaac tggttatcgc
    45121 tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
    45181 caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
    45241 gcctgccttc ccgtataccg ccgattactt ttctggcctg accgccacca cggcggcgct
    45301 ggcagcactg cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta
    45361 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
    45421 gtgcccgcgc atgagcaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg
    45481 cgccgacggc tacatcgtga tggaactggt gggcattacc caaattgaag agtgctttaa
    45541 agatattggc ctcgcacatc tgctcggtac gccggaaatc ccggaaggca ctcagcttat
    45601 ccaccgtatc gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc
    45661 ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
    45721 agtactgacc gtaccggaac tggaaagcaa tccacagtat gttgcccgtg aatcaatcac
    45781 tcagtggcaa acgatggatg gtcgcacctg caaagggccg aacatcatgc cgaaattcaa
    45841 aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
    45901 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc
    45961 caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata
    46021 acaaacgaac aaaaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc
    46081 aaatgtggga cgatctggcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca
    46141 gcggcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg
    46201 caaacctgtt ttatacgctg gggattcgca aaggcgacaa ggttgcacta catctcgaca
    46261 actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc
    46321 cgattaacgc ccgcctgtta cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt
    46381 gcctgctggt gaccagtgcg caattctatc ctatgtatca acagattcag caggaagatg
    46441 ccactcaatt gcggcacatt tgcctgacag atgtggcact tcccgctgat gatggcgtga
    46501 gttcgtttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat
    46561 tgactgacga tacggcggaa attctcttca cctccggcac cacctcccga ccgaaaggtg
    46621 tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac
    46681 tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta
    46741 ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt gctggtcgag aaatacagcg
    46801 cccgcgcctt ctggggacag gtgcagaagt accgcgccac cattaccgaa tgtattccga
    46861 tgatgatccg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc
    46921 gggaagtgat gttttatctc aacttgtcgg agcaggaaaa agatgcgttt tgtgaacgct
    46981 tcggcgttcg cttgctgacg tcttatggga tgacggaaac cattgtgggc attatcggcg
    47041 atcgccctgg cgataaacga cgctggccgt cgattggtcg ggcggggttt tgctacgaag
    47101 cggagatccg cgacgatcac aatcgcccgc tcccggcagg tgagatcggt gaaatctgta
    47161 ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg
    47221 cgaaagtgct ggaagccgac ggctggctac ataccggcga taccggatac tgcgacgaag
    47281 agggcttttt ttatttcgtc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg
    47341 tctcctgcgt ggagctggaa aatatcatcg ccacccatcc aaaaattcag gatatcgtgg
    47401 ttgtgggtat taaagattcg attcgcgatg aagccatcaa agcatttgtg gtgctgaatg
    47461 aaggtgaaac attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat
    47521 ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa
    47581 taattagaaa gaatctgaaa taaatgaaac agcagggaac taccctgcca gctaataatc
    47641 acgctataaa acaatatgca ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat
    47701 ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta
    47761 cccttgatcg tccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag
    47821 tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag
    47881 agaagttctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg
    47941 ctgactttgg cccgggtgga tttgcgggat taaccgaaat tttcaatctc gacaaaccgg
    48001 ttatcgcagc tgtgaacggc tatgcctttg gcggcggttt cgaactggcg ctggcggcag
    48061 attttattgt ttgtgccgat aacgccagct tcgccctgcc ggaagccaag ctgggcatcg
    48121 ttcctgacag cggcggcgtg ctgcgtctac cgaagatcct gccgcctgcc atcgtcaatg
    48181 aaatggtgat gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg gggatagtca
    48241 accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggcg cagcagctgg
    48301 ttaacagcgc cccgctggcg attgcggcgc tgaaagagat cttccgcacc accagcgaaa
    48361 tgccggtaga agaagcgtat cgctatattc gcagcggcgt gttgaaacac tatccgtcgg
    48421 ttctgcattc ggaggatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccgg
    48481 tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggtggt
    48541 tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc
    48601 cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca
    48661 agcgggagcc aatattcagg atggctgcat tatgcatggc tactgcgaca ctgacaccat
    48721 cgttggggaa aacggccata tcgggcacgg agcgatcctg catggttgtg tgattggtcg
    48781 cgatgcattg gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag
    48841 cattgttgcc gccatgagct ttgtcaaagc aggctttcac ggcgagaaac gccagttgtt
    48901 gatgggtacg ccagcccgcg ctgtacgcag tgttagtgac gacgagttac actggaaacg
    48961 gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tacatgaaac
    49021 acagccactg aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac
    49081 gcctaaacgg tgatcttgcc ggatgcgacc tggttgctgg ataagatgct acaacatcgc
    49141 atctggcaag caggacgccc ggtatcaacg agataaaatt aacgacgcat actctttgac
    49201 aacatcatct gccacttctg ctcgcggtta agttccgtga gcggaacggc aggaatacgc
    49261 ggcgtctttc ttacgccgac taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt
    49321 tgctcatcga tatcgaccac ttttaccagt cgctggcact ggcatccccg tccttccagc
    49381 ttattaggga tcatcttaac ttcgcagctt atttctgcga cttccgacag aatataagtc
    49441 agggtattaa ccgccttgct gtgttcaata tcgaaatgaa tagagatctc tcttgctatc
    49501 acccaccgat tttcagccat catccattcg gcgattaaca agtagagtgg tttttcaaca
    49561 tatccttcac acatacgaac tccagtttca caaattaaaa gtctggctat gactcgtttt
    49621 gaagcaatta aacaaggcca tattaaaatt gtggatatat cgatagtatg caacttcacc
    49681 gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc cccaaaagat
    49741 ttacttaatg ggcaaaaaaa acggtcctca tcagagaacc gtattactta ttcgatcaaa
    49801 ttagcggatg aaaaatatcc gccatgacac gctattattt gatctgcgcg tgcatttcct
    49861 gcaccgaaat tactttttcg gtcgcgtcgg cattcagcgc catcgcggta gcgaaaccac
    49921 cgttcagggt ggtgtcataa tgcactttat attgcagcgc actgcgacgg atcacgcggg
    49981 agtcttcaat cgcacgacgg cctgaagtgg tgttgatgat gtaggtatat tcgccattct
    50041 tgatgcggtc ctgaatgtgc ggacggcctt catgcacctt gttcaccaga cgcggattaa
    50101 tgcccgcttc gcccagcaca atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt
    50161 tcagcagttt tgccgccagg tccaccacgc gttctttatc gccttcgcgc acggaaagca
    50221 acgcacgacc gtgtttcttc atggtggagt tgctgcccag ctgcgctttg gcaaacgctt
    50281 cagcgaaggt gcggcccacg cccatcactt ccccggtgga gcgcatttct ggccctaaca
    50341 gcgggtcaac acccgggaat ttattgaacg gcagcaccac ttctttcacc gagtagtacg
    50401 gcgggataac ttctttggtt acgccctgct cagccagcga tttgccagcc atcacacgcg
    50461 ccgccacttt tgccagcggt acgccggtgg ctttggagac gaacggaacg gtacgcgccg
    50521 cacgcgggtt aacttcaatc aggtagactt cgttgttttt caccgcaaac tgcacgttca
    50581 tcagaccgcg cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc atcacatcct
    50641 gaatttcctg acttaaggtg taggctggca gagaacatgc ggagtcaccg gagtgcacgc
    50701 ccgcctgctc gatgtgctcc atgatgccgc caatcagcac catttcgccg tcgcagatgg
    50761 catccacgtc aacttctacc gcgtcatcaa ggaagtgatc cagcaacact ggcgcatcgt
    50821 tagacacgct gaccgccgtc tggaagtagc gacgcaggtc agcttcgtca tagacgattt
    50881 ccatcgcccg accgccgaga acgtaagacg gacgtaccac cagcgggtag ccaatctctt
    50941 tcgccttctc taccgccatt tcaatagcgg taacggtggc gttcgccggt tgtttcagtt
    51001 tcagacgctc aaccgcatgc tggaagcgtt cacggtcttc tgcacggtcg atagcatccg
    51061 ggctggtgcc gataaccggt acgccagcag cttccagcgc gcgcgccagt ttcagcgggg
    51121 tctgaccgcc gtactgaacg ataacgcctt tcggcttctc aatacgcacg atttctagca
    51181 catcttccag tgttaccggc tcgaagtaga ggcgatcgga agtgtcgtag tcggtggaga
    51241 cggtttccgg gttacagtta accataatgg tttcgtaacc gtcttcgcgc agtgccagcg
    51301 aggcgtgtac acagcagtag tcgaattcga taccctgacc gatacggttc gggccgccgc
    51361 cgagaaccat gattttttca cggtcggtag acggattcgc ttcgcactct tcttcataag
    51421 tggagtacat gtaagcggtg tcggtggcga actctgccgc acaggtatcc acgcgcttat
    51481 aaaccgggtg caggtcatac tggtcgcgca gcttgcggat ttccgcttcg cgcacgcccg
    51541 ccagttttgc cagacgcgca tcggcaaagc ctttgcgttt cagctggcgc aggaattcag
    51601 cgttcaggcc agtgatgccc acttccgcta ctttctcttc cagacgcacc agttcttcaa
    51661 tctgtaccag gaaccagcgg tcaatattgg tcaggttgaa cacgccgtcc acagacaggc
    51721 ccgcacggaa ggcatcggcg atgtaccaga tacgctctgc gccagcatct ttcagttcgc
    51781 gacggatttt ggttaacgct tccgggtcat cgaggctcac tttcgggtcg aatccagtct
    51841 caccgacttc caggccgcgc agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg
    51901 ccatcacttc gccaaccgat ttcatctgag tggtcagacg gtcgttagca ccagcgaatt
    51961 tttcgaagtt gaagcgagga attttagtaa ccacatagtc gatggacggc tcgaaggagg
    52021 ccggagtacg tccgccagtg atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca
    52081 gtttcgccgc cactttagca atcgggaaac cggtcgcttt cgacgccagc gccgaagaac
    52141 gggacacgcg cgggttcatt tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa
    52201 actggacatt ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt
    52261 tacgcatgat ttgatattct ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt
    52321 caccggtatg gatgcccatc gcatcaaagt tttcgataga gcagacgatg atgcagttgt
    52381 cgtttttatc acgcaccact tccatctcgt actctttcca gccaatcagc gactcatcaa
    52441 tcagcaactc tttggtcgga gagagatcca gaccgcgggc gcaaatttct tcaaactctt
    52501 cgcggttata agcgataccg ccgccgctac cgcccatggt aaaggatggg cgaataatgc
    52561 acgggaagcc cacgtcagcg gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg
    52621 aacgcgcggt ttccagacca attttcttca tcgctacgtc gaaacgacgg cggtcttctg
    52681 ctttatcaat cgcatcggcg gtcgcaccaa tcatagtcac gccgaactct tccaacacgc
    52741 cctgacgctc cagctccagc gcgcagttca gcgccgtctg accgcccatc gttggcagca
    52801 ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac ttcccagtga atcggctcga
    52861 tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt cgccgggttg gagttcacca
    52921 gaatgacccg gtaaccctct tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa
    52981 actcacacgc ctgaccgata acaatcgggc ccgcacccag aatcaggata ctttttatat
    53041 ctgtacgttt tggcatggct cttttactcc tgattactta gcggttttac ggtactgctc
    53101 aattaactcg ataaagtggt cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc
    53161 agggtgcccc tggaagctga acgccggttt atcggtgcga tgaatgccct gtaacgtacc
    53221 gtcgaacagg gatttatgcg tgacacgcag gtttgctggt aatgttgctt cgtccaccgc
    53281 aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc tcaacatctt taaccggatg
    53341 gttgccgccg tggtgaccaa atttcatttt gacagtcttc gcaccgctcg ccagcgccag
    53401 cagctgatga ccgaggcaga tgccgaagac cggaatatcg gtttcgagga atttctggat
    53461 ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga ccgttggaga ggaagatgcc
    53521 gtctggattc attttcagca catcttccgc agaagtttgc gccggaacga tggtcaggcg
    53581 acagcctctg tccaccaaca tgcgcaggat gttgcgcttg gcaccaaaat cataagccac
    53641 gacgtggaac ggcagctcgt cttctttttt cgcttctggc aggccgccgg tcaacgtcca
    53701 gctcccttgt gtccagctat aggcttctgc ggtggtcact tcttttgcca gatccatgcc
    53761 attcagacct gggaacgcgc gggctttttc taacgccaac gccgcatccg ggttatcgcc
    53821 tgcgataatg cagccattct gtgcgccttt ctcgcgcagt aaacgcgtca gcttacgggt
    53881 atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg taagaagaga ggtcttcggt
    53941 attacggaag ttgctggcaa tcagcggcag gtcgcgaatc accagacctt gtgcatgtac
    54001 ctgagaagat tcttcatcgg cgtcattagt gccgacattg ccaatatggg gataagtaag
    54061 agtaacgatt tgacgagaat aggaaggatc agtgaggatt tcttgataac cggtcattga
    54121 agtattgaaa acgacttccc caaccgccga acctgttgcc cctatggccc gaccgtgaaa
    54181 ctgggttccg tcttccagaa ccaatagcgc tgacttaatc aaaacaccct ccagagaata
    54241 ttcactcact ttatttgcat attaattcac aatgatgacg taaatcaatg caaatctgct
    54301 taccgacgaa tttctggcaa acggcggcat tctggagata tgggcgctaa aagtcaactt
    54361 aatggcggga ttttttatat tattttgtgg ctcatgcatg ttttacaggc attaagcggc
    54421 aaaaagatca tataacctgt tctggaaaac gcttgcgcag caagaagatt gcatgaaata
    54481 aaagcgagta gggaaaagtg gaccaaatgg tcaaaattta cattaaaaca acaaaaaccg
    54541 aagacaaaac atgtaatttt tggcacttat agacaattta aaaacataac actaaaaat
//
LOCUS       DAZUFB010000021        52898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZUFB010000021 DAZUFB010000000
VERSION     DAZUFB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.021"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..560
                     /gene="espX1"
                     /locus_tag="ACHY3M_001459"
     CDS             <1..560
                     /gene="espX1"
                     /locus_tag="ACHY3M_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFS4316400.1"
                     /translation="ELNEQNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLPN
                     ILCFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNK
                     HLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKT
                     SSEMRKYLKSINECMFLMKNIAHQN"
     gene            794..2146
                     /locus_tag="ACHY3M_001460"
                     /pseudo
     CDS             794..2146
                     /locus_tag="ACHY3M_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2159..3118
                     /locus_tag="ACHY3M_001461"
     CDS             2159..3118
                     /locus_tag="ACHY3M_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFS4316401.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(3156..4055)
                     /gene="nhaR"
                     /locus_tag="ACHY3M_001462"
     CDS             complement(3156..4055)
                     /gene="nhaR"
                     /locus_tag="ACHY3M_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFS4316402.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(4121..5287)
                     /gene="nhaA"
                     /locus_tag="ACHY3M_001463"
     CDS             complement(4121..5287)
                     /gene="nhaA"
                     /locus_tag="ACHY3M_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFS4316403.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5873..6025
                     /gene="mokC"
                     /locus_tag="ACHY3M_001464"
     CDS             5873..6025
                     /gene="mokC"
                     /locus_tag="ACHY3M_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFS4316404.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(6129..7244)
                     /gene="dnaJ"
                     /locus_tag="ACHY3M_001465"
     CDS             complement(6129..7244)
                     /gene="dnaJ"
                     /locus_tag="ACHY3M_001465"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4316405.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGADFSDIFGDVFGDIFGGGRG
                     RQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCH
                     GSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPAGVDTG
                     DRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALGGEIE
                     VPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLNEKQKQ
                     LLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(7333..9249)
                     /gene="dnaK"
                     /locus_tag="ACHY3M_001466"
     CDS             complement(7333..9249)
                     /gene="dnaK"
                     /locus_tag="ACHY3M_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4316406.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            9626..10030
                     /gene="yaaI"
                     /locus_tag="ACHY3M_001467"
     CDS             9626..10030
                     /gene="yaaI"
                     /locus_tag="ACHY3M_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFS4316407.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            10056..10769
                     /gene="msyB"
                     /locus_tag="ACHY3M_001468"
     CDS             10056..10769
                     /gene="msyB"
                     /locus_tag="ACHY3M_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS4316408.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10918..11484
                     /gene="satP"
                     /locus_tag="ACHY3M_001469"
     CDS             10918..11484
                     /gene="satP"
                     /locus_tag="ACHY3M_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFS4316409.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(11519..12106)
                     /gene="mog"
                     /locus_tag="ACHY3M_001470"
     CDS             complement(11519..12106)
                     /gene="mog"
                     /locus_tag="ACHY3M_001470"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFS4316410.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(12221..13174)
                     /gene="tal"
                     /locus_tag="ACHY3M_001471"
     CDS             complement(12221..13174)
                     /gene="tal"
                     /locus_tag="ACHY3M_001471"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS4316411.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            13453..14883
                     /gene="yaaJ"
                     /locus_tag="ACHY3M_001472"
     CDS             13453..14883
                     /gene="yaaJ"
                     /locus_tag="ACHY3M_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFS4316412.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14953..15729
                     /gene="yaaA"
                     /locus_tag="ACHY3M_001473"
     CDS             14953..15729
                     /gene="yaaA"
                     /locus_tag="ACHY3M_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFS4316413.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(15883..16179)
                     /gene="yaaX"
                     /locus_tag="ACHY3M_001474"
     CDS             complement(15883..16179)
                     /gene="yaaX"
                     /locus_tag="ACHY3M_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFS4316414.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(16393..17679)
                     /gene="thrC"
                     /locus_tag="ACHY3M_001475"
     CDS             complement(16393..17679)
                     /gene="thrC"
                     /locus_tag="ACHY3M_001475"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFS4316415.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(17680..18612)
                     /gene="thrB"
                     /locus_tag="ACHY3M_001476"
     CDS             complement(17680..18612)
                     /gene="thrB"
                     /locus_tag="ACHY3M_001476"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFS4316416.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(18614..21076)
                     /gene="thrA"
                     /locus_tag="ACHY3M_001477"
     CDS             complement(18614..21076)
                     /gene="thrA"
                     /locus_tag="ACHY3M_001477"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFS4316417.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(21102..21221)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(21157..21222)
                     /gene="thrL"
                     /locus_tag="ACHY3M_001478"
     CDS             complement(21157..21222)
                     /gene="thrL"
                     /locus_tag="ACHY3M_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFS4316418.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(21436..22122)
                     /gene="yjtD"
                     /locus_tag="ACHY3M_001479"
     CDS             complement(21436..22122)
                     /gene="yjtD"
                     /locus_tag="ACHY3M_001479"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS4316419.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(22522..22662)
                     /gene="yjjY"
                     /locus_tag="ACHY3M_001480"
     CDS             complement(22522..22662)
                     /gene="yjjY"
                     /locus_tag="ACHY3M_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFS4316420.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            22758..23474
                     /gene="arcA"
                     /locus_tag="ACHY3M_001481"
     CDS             22758..23474
                     /gene="arcA"
                     /locus_tag="ACHY3M_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFS4316421.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(23533..24885)
                     /gene="creD"
                     /locus_tag="ACHY3M_001482"
     CDS             complement(23533..24885)
                     /gene="creD"
                     /locus_tag="ACHY3M_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFS4316422.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(24943..26367)
                     /gene="creC"
                     /locus_tag="ACHY3M_001483"
     CDS             complement(24943..26367)
                     /gene="creC"
                     /locus_tag="ACHY3M_001483"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFS4316423.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(26367..27056)
                     /gene="creB"
                     /locus_tag="ACHY3M_001484"
     CDS             complement(26367..27056)
                     /gene="creB"
                     /locus_tag="ACHY3M_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFS4316424.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(27069..27542)
                     /gene="creA"
                     /locus_tag="ACHY3M_001485"
     CDS             complement(27069..27542)
                     /gene="creA"
                     /locus_tag="ACHY3M_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFS4316425.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            27753..28622
                     /gene="robA"
                     /locus_tag="ACHY3M_001486"
     CDS             27753..28622
                     /gene="robA"
                     /locus_tag="ACHY3M_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFS4316426.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(28619..29266)
                     /gene="gpmB"
                     /locus_tag="ACHY3M_001487"
     CDS             complement(28619..29266)
                     /gene="gpmB"
                     /locus_tag="ACHY3M_001487"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFS4316427.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            29318..29839
                     /gene="yjjX"
                     /locus_tag="ACHY3M_001488"
     CDS             29318..29839
                     /gene="yjjX"
                     /locus_tag="ACHY3M_001488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFS4316428.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(29925..30251)
                     /gene="trpR"
                     /locus_tag="ACHY3M_001489"
     CDS             complement(29925..30251)
                     /gene="trpR"
                     /locus_tag="ACHY3M_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFS4316429.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(30341..32278)
                     /gene="sltY"
                     /locus_tag="ACHY3M_001490"
     CDS             complement(30341..32278)
                     /gene="sltY"
                     /locus_tag="ACHY3M_001490"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFS4316430.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            32489..34156
                     /gene="ettA"
                     /locus_tag="ACHY3M_001491"
     CDS             32489..34156
                     /gene="ettA"
                     /locus_tag="ACHY3M_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFS4316431.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(34463..35695)
                     /gene="nadR"
                     /locus_tag="ACHY3M_001492"
     CDS             complement(34463..35695)
                     /gene="nadR"
                     /locus_tag="ACHY3M_001492"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFS4316432.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(35716..37098)
                     /gene="radA"
                     /locus_tag="ACHY3M_001493"
     CDS             complement(35716..37098)
                     /gene="radA"
                     /locus_tag="ACHY3M_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4316433.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(37147..38115)
                     /gene="serB"
                     /locus_tag="ACHY3M_001494"
     CDS             complement(37147..38115)
                     /gene="serB"
                     /locus_tag="ACHY3M_001494"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFS4316434.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            38221..38865
                     /gene="ytjB"
                     /locus_tag="ACHY3M_001495"
     CDS             38221..38865
                     /gene="ytjB"
                     /locus_tag="ACHY3M_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFS4316435.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            38893..39909
                     /gene="lplA"
                     /locus_tag="ACHY3M_001496"
     CDS             38893..39909
                     /gene="lplA"
                     /locus_tag="ACHY3M_001496"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFS4316436.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            39941..40204
                     /locus_tag="ACHY3M_001497"
                     /pseudo
     CDS             39941..40204
                     /locus_tag="ACHY3M_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(40365..41084)
                     /gene="deoD"
                     /locus_tag="ACHY3M_001498"
     CDS             complement(40365..41084)
                     /gene="deoD"
                     /locus_tag="ACHY3M_001498"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4316437.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(41164..42387)
                     /gene="deoB"
                     /locus_tag="ACHY3M_001499"
     CDS             complement(41164..42387)
                     /gene="deoB"
                     /locus_tag="ACHY3M_001499"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4316438.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(42439..43761)
                     /gene="deoA"
                     /locus_tag="ACHY3M_001500"
     CDS             complement(42439..43761)
                     /gene="deoA"
                     /locus_tag="ACHY3M_001500"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFS4316439.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(43888..44667)
                     /gene="deoC"
                     /locus_tag="ACHY3M_001501"
     CDS             complement(43888..44667)
                     /gene="deoC"
                     /locus_tag="ACHY3M_001501"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4316440.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            44925..46475
                     /gene="yjjI"
                     /locus_tag="ACHY3M_001502"
     CDS             44925..46475
                     /gene="yjjI"
                     /locus_tag="ACHY3M_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFS4316441.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITHAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVHDKFFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSNIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            46447..47310
                     /gene="yjjW"
                     /locus_tag="ACHY3M_001503"
     CDS             46447..47310
                     /gene="yjjW"
                     /locus_tag="ACHY3M_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFS4316442.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEGVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(47423..48205)
                     /gene="yjjV"
                     /locus_tag="ACHY3M_001504"
     CDS             complement(47423..48205)
                     /gene="yjjV"
                     /locus_tag="ACHY3M_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4316443.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(48202..49275)
                     /gene="yjjU"
                     /locus_tag="ACHY3M_001505"
     CDS             complement(48202..49275)
                     /gene="yjjU"
                     /locus_tag="ACHY3M_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFS4316444.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(49397..49558)
                     /gene="ytjA"
                     /locus_tag="ACHY3M_001506"
     CDS             complement(49397..49558)
                     /gene="ytjA"
                     /locus_tag="ACHY3M_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFS4316445.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(49685..50290)
                     /gene="osmY"
                     /locus_tag="ACHY3M_001507"
     CDS             complement(49685..50290)
                     /gene="osmY"
                     /locus_tag="ACHY3M_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFS4316446.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(50683..52269)
                     /gene="prfC"
                     /locus_tag="ACHY3M_001508"
     CDS             complement(50683..52269)
                     /gene="prfC"
                     /locus_tag="ACHY3M_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4316447.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEGLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            52489..52737
                     /locus_tag="ACHY3M_001509"
     CDS             52489..52737
                     /locus_tag="ACHY3M_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4316448.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(52734..52820)
                     /locus_tag="ACHY3M_001510"
                     /pseudo
     CDS             complement(52734..52820)
                     /locus_tag="ACHY3M_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
BASE COUNT        12968 a        14008 c        12960 g        12962 t
ORIGIN      
        1 atgaacttaa cgagcaaaac atccagtatt tgactgccct taatattaat atacataaaa
       61 tgttgctatc aaatataaca atagaaaaga gcgacctttc atatgggtat tattttggtt
      121 gtgtattacc caatatatta tgttttgaaa gcgacttatc aaacaccatc ttttcaaatg
      181 gagaaattaa taatctcttc attaaaaaaa gcaacatatt tggagcatca ttcactaata
      241 ccagaataaa aaatttgctc tgtgaagata ttatgcctgg tcgatggacc actcaacttg
      301 tcaataagca cttaggttac agatacacag gagtattcaa aaccctggca agtattgatg
      361 acaaaccgtc ccgtttcgaa attttgatac cattggtaca aactttagtt cgcgacaatg
      421 taaagctaaa taatgatgtg tataaagaac ttaataagtt tatgcacgat tacgacaaaa
      481 catcatcaga aatgagaaaa taccttaaaa gcatcaacga gtgtatgttc cttatgaaaa
      541 acatagctca tcaaaattaa aaaatctcaa aaaagaagta atttcagaat gaaattaact
      601 gtgatgatta ttaattcact acagaggaat aaaaaacaat ctgaagcatg ctctgaaaca
      661 tcctactctg tcactctaat gatggcacct tgctcttctt ataaagaagg gaacaattaa
      721 gaatattaaa tcttcaaact ttcatcaact tcacatcaag aaattaaaaa atttaaacat
      781 taatctaaat aataacaacc tgatcacata atcgggtacg attttatccg ataactataa
      841 tgctataact ctgggaaatg gttggaatac accgctgggt gccatctctt tttacgctgc
      901 ccgatcgagt agtaagttaa ataatgatac cagacacgaa ggtacgagct atcaggtcgc
      961 ctataataaa tatctagtac aaacagccac tcgatttagc gtggccgcct ggcgttatgc
     1021 ttcgcaggat tacaggacat tcagcgacca tctttacgaa aatgataaac actatcatca
     1081 gagtgattat gatgattttt atgatattgg cagaaaaaat agcctttctg ctaatattat
     1141 gcaaccttta tccaataatc tgggaaatgt atcattaagt gcgctttggc ggaattactg
     1201 ggggcgaagt ggaaatgcta aagattacca attcagttac tccaataact ggcaacacat
     1261 tagttatact ttctctgcca gccaatctta tgatgaaaat aataaagaag aggagcgttt
     1321 taatctgttt atctccattc ctttctactg gggggatgat attgccaaaa cacgtcacca
     1381 aattaactta tcgaattcga cctcattttc caaagatggc tattcctcca acaatactag
     1441 aattactggc atagccggtg aacatgatca gttaaattat ggtatatatg ttaatcagca
     1501 acaacaaaat aatgatacct cgcttggtac gaatttaagc tggagaactc ccatcgccat
     1561 aatagatggc agctatagtc attctaaaaa cgcctggcaa agtggtggaa gtattagtag
     1621 tggattagtt gtctggcccg gtggtattaa tatcactaac cagttatccg atacatttgc
     1681 aattctggat gcgcctgggt tagaaggtgc acatattaat ggacaaaaat acaatcgaac
     1741 aaacagcaaa ggccaggttg tttacgacct gataatacct catcgtgaaa accatctggt
     1801 acttgatata gcaaacagtg aaagtgaaac agaattgcag ggcaatcgtc aaattattgc
     1861 accttaccgt ggagcagttt cttatgtgca gtttacaact gaccaacgta agccctggta
     1921 tatacaggca ctgcgtcccg atggttcgcc attaaccttt ggctatgacg tactggatct
     1981 ccaggaaaat aatattggag tcgttggcca gggtagtcgc ctttttattc gcgtagatga
     2041 aattcccact ggcataaaag ttgctctcaa tgatgaacag aacttattct gtactattac
     2101 ttttcaacac gttatcgatg aaaacaaaac atatatatgc cagtaaaagg agtttactat
     2161 gaagtggctt ttattaatca ccttgagtct atattctttt attgtgcaaa gtgctccctg
     2221 tgcattgacc aatgtcggtg aacaacgtgg aacatatata ctcaagccac tgagtatgaa
     2281 aggtaatcta accgccgaaa aaattgaaaa caccggaaat atcattgagt gtacagactt
     2341 gcaagagaca accaacgcca ttaaagttca ttttttaaca cagaacgctt taaggtttaa
     2401 agtcggaagt cgtcattata ttattaattt cccctttgaa tctgacggta aaaaaataga
     2461 gcttccaggg aagtcatctt ataatttaga agaaatgcta tctgcgataa atttcaccat
     2521 tccatattct atcgcatctg tatcagatag ttctaacacc gcagacattt ttcctggaac
     2581 tccttttcct ttgttgttcg aaattgaagt gagttcatgt acagggaata atactaattg
     2641 taatagcgtc cgctttaatt ataatttaaa tactatttta caggtaaatt taatgacctg
     2701 tggttttgaa gatcaatcca tcgacactgg acgatttgta ctgtctcgta tcaataatga
     2761 aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt
     2821 ttttgaaccc agtgatgttg aatattactt tgaaccagtg acgatacagg agtcaggaac
     2881 cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa taggctttca
     2941 actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca
     3001 gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa
     3061 taatgtctca agcgggcaaa taatgagccg agtcaaaatc gtggttatgt acaactaatc
     3121 tatctccccc accttaattt ggggggaggt gccgattaac gcgccgctgg actaaaaagc
     3181 gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca
     3241 aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta
     3301 tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca
     3361 ccaaaagctt tcatcaacgc ggcatcatca aactcgccga ggatttctac gtttaatccc
     3421 tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa
     3481 agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag
     3541 aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct
     3601 atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt
     3661 tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac tgcggcgtta
     3721 agtacgctac tgaccaggcg tttggaaagt gcatcagcca caccaacgtc aaacaataaa
     3781 ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta
     3841 tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt
     3901 ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt
     3961 ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag
     4021 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     4081 gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac
     4141 gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga
     4201 gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga
     4261 tagacatagt aaaaccgata ccgcacagga tccccaccgc cataatttgc tgataagtcg
     4321 ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc
     4381 ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc
     4441 catccagcgt gacaccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
     4501 tcagatacgc cacccacggg tgtaacacat gctccagtcg cttcgctgga gaacgcccat
     4561 gcttctcttt caaaggaata aagaagccga caattacccc cgccagggtt gcgtgaaccc
     4621 ccgatttcaa caccgcagtc cacaacacta cgccaaccag aatatagacg cccgtgcggc
     4681 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag
     4741 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
     4801 cgataatagc cagagccatc aaaaagatct tcagcgctaa cggaacacga cttcccaaca
     4861 gcgccagcac accaagtgca aaggcaatgt cagtagccgc cgggatcgcc cacccttcgc
     4921 gggtaatcgg atcggcatag ttaaaggcca gatagagtaa tgccggaaca atcatcccac
     4981 caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca
     5041 gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta
     5101 tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct
     5161 ccagaaagtc gtgataccat ccactggttg cgccgctatt ggccataatc atcgccagga
     5221 tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
     5281 gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcaca
     5341 aatcatcgtt aaaatcagat tatagtgtag gaataactcg ttttttacga tccattgaag
     5401 aatttgcgcc atcagcggtg agatgagttt ttgacggctg taagcgctca cgagtgaccc
     5461 taagcccaga taatcatagc ttaaggtgag gatttctgag ggattaatta ggaaaagtaa
     5521 taattaattt cttgtcagtc ggcgttattt gttaggacaa tatttcaaaa ataatccagc
     5581 ttgtcgtcaa taattgcgat aaagagataa attaaagtta aataattaaa tcattaaaaa
     5641 attacgccac aaaaatgcga ggcgtcttca ggattagaat cgataaaaca gaatgggtta
     5701 aagagagcga tataatagcg gcgggtactt gaggctatct gtctcaggca ttagctgaac
     5761 ggcagataga gaaaagcccc gagtgatatt ttactatcaa cccgaggccc cctatatgct
     5821 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
     5881 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
     5941 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
     6001 tttcacggct tacgaatccg agtaagtgca acggcgggga gtgatccccg ccacttttca
     6061 ggtgctcgca tatcttcaac gcaccctaat tttacccaag cctgcccacg ggcaggcttt
     6121 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
     6181 gagcgcgggc tgttgtgctc accggttggg ccaccgaagc tttcttgcag ctcttgcagc
     6241 agctgtttct gcttctcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
     6301 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaaaagctta
     6361 ccggtctggg tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg
     6421 atttcgccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata gaggttgttg
     6481 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
     6541 ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
     6601 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     6661 catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc
     6721 tgtacagcga agaatccctg acgcatctgc acctgaccag aaccatgaca ggtcggacag
     6781 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     6841 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     6901 ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata
     6961 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgcccat accaccttgc
     7021 tcaaacgcag catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact
     7081 tcataagctt ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg
     7141 tccgggtggt atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct
     7201 gttttggaaa cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc
     7261 ctagatgaat gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg
     7321 tttatagggc gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc
     7381 atctttcgcg ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg
     7441 ctgggcgatt tccatcagtt tctgggaaac ctgtgccagc tcctgcattt tcgcttcgat
     7501 agcggctttg tcttcacctt tcagagcagt ttccagtgca gtcagtgcag actcgatagc
     7561 agttttgtcg tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg
     7621 cagcagatgg tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc
     7681 ggcgttagct tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga
     7741 agccttgatg gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcag
     7801 gataccgtca gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg
     7861 gttgatacca tccaggttga actgacccag agatttgtta tcagccgcac gtttacgttc
     7921 accctgcagc acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg
     7981 gctgtgcttg gtcgggatag tggtgttttt cgcgatcagc gtcgtcatca caccgcccat
     8041 ggtttcgata cccagagaca gcggggtaac gtccagcagc agtacgtctt tcacgtcacc
     8101 agtcagaaca cccccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc
     8161 tttacgcggc tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt
     8221 ctgaccacca acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag
     8281 tgcaactttc agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt
     8341 cgcacgagtc actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg
     8401 caggttaacg tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc
     8461 tttcaggcgc tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa
     8521 ttcttcaacc aggtagttga tcagacggct gtcgaagtct tcaccaccca ggtgggtatc
     8581 accgttggta gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat
     8641 agaaatatcg aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc
     8701 tttgtccaga ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc
     8761 cagaccagcg atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc
     8821 cggtacggtg ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt
     8881 catttttttc agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc
     8941 gacccatgcg tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg
     9001 ctgtacttct tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt
     9061 ttgcgggttc gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg
     9121 ggtataggca ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg
     9181 agtggtgcca tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat
     9241 tttacccatc taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat
     9301 aggggcgaaa cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc
     9361 ggttgactac taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt
     9421 gtgcttttgc gcagaaattt cgcgatatag caggacaaga cgtgaaactg acagcaattt
     9481 tattcatata aagaatgaat cgcagccaag acattcatca aaaaattgta ataaaaagaa
     9541 aacattacgc acctgaatct tctctttatc agcagtaaac tagtgggtat ccatccccct
     9601 acctctcccc actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc
     9661 gattagtctg atgctgtgct gcacggcgca ggcaaacgac cataaaatcc tcggcgtcat
     9721 tgcgatgcca cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gccgaattgt
     9781 taaacgcata caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta
     9841 tttcaaagcc gcccgtagcg ccagtcagag cctgaatatt ccttcagaaa taaaagaagg
     9901 gcaaaccact gactggatca acattaacag cgataacgac aataaacgct gcgtctcaaa
     9961 aatcaccttt tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg
    10021 cgacgactaa cgccagaata tagaagccac aaaaaatgaa tgttaattac ctgaatgatt
    10081 cagatctgga ttttctccag cattgcagtg aggaacagtt ggcaaatttc gcccgattgc
    10141 tcacccataa tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt
    10201 ttaaatctat ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag
    10261 aattacagca ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt
    10321 atcgggccat tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt
    10381 ctacgtttga gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata
    10441 tggacgagga acataaacag gagtttctgc acgcggtcga tgcgagggtg aatgagctgg
    10501 aagagctgct gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc
    10561 tttccagcca actgacccgc attttacgca cccacgcagc aatgagcgta cttgggcatg
    10621 gtttgctgcg cggcgcgggg ctgggaggcc ctgtaggtgc ggcactaaat ggggttaaag
    10681 cggtcagcgg cagcgcctat cgcgtgacga ttccagccgt actgcaaatc gcctgcctgc
    10741 gccggatggt tagcgccact caggtctgat accggaaacc ggggaaaatt ttcattttcc
    10801 cctcaaaaga tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac
    10861 tacagttatt cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg
    10921 ggcaacacta agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc
    10981 attctgctta acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg
    11041 ggcattttct acggcggcat cgcgcaaatt tttgctggtc tgctggagta caaaaaaggc
    11101 aacactttcg gtttaaccgc attcacctct tacggttctt tctggctgac gctggttgcg
    11161 attctgctga tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc
    11221 tatctgggtc tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca
    11281 cgcgttctgc aattcgtttt ctttagcctg accgtgctgt ttgccctgct ggcgatcggt
    11341 aacattgccg gtaacgccgc aatcatccac tttgccggct ggattgggct gatctgcggt
    11401 gccagcgcaa tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt
    11461 ctgccgattg gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt
    11521 attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc tgcaaccact tccggtgccg
    11581 tttcaacgta tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt
    11641 gtaccacaac gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg
    11701 gctgaccggg taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa
    11761 ggatcgcagt tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca
    11821 tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag
    11881 ttccgcccgt ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt
    11941 gctcgatgat cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta
    12001 gcgccgatgt cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg
    12061 atgcgcgatc agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt
    12121 ttaaacagtc tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga
    12181 agaggtagcg tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca
    12241 ttttttccag tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta
    12301 ctgccattgg atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac
    12361 gcgctttcac ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca
    12421 gctctttcag cagtgccggt gcgatggtca gacggtcgca gcctgccagt tccagaattt
    12481 cgccgatgtt acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt
    12541 actggtagat ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct
    12601 tatcggtatt cgctttgtac cagtcaagaa tacggccaac aaacggcgag atcaggaaca
    12661 cgcccgcttc cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt
    12721 tgatgccttc tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt
    12781 tgatcagaat acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg
    12841 ctttcgcaat tgacgcttca gtgtcatagg aaagacgcgc atcaacttca gttgagatac
    12901 ggcccggaac cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt
    12961 ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa
    13021 tcaacttacg gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg
    13081 catcctgcgg ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg
    13141 tgtactgacg aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg
    13201 ttagggggat gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc
    13261 gacaagacgg ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc
    13321 ccgccatttt tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc
    13381 gtatatactt cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata
    13441 agagggatat gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta
    13501 atgatttacc tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag
    13561 tttcgctaca tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc
    13621 ggtttaacct catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac
    13681 ctggccggcg ttgcgctggc aattaccgcc ggtggacctg gagccgtctt ttggatgtgg
    13741 gttgccgcgt ttatcggcat ggcgacctcg tttgccgaat gttctctggc acaactttat
    13801 aaagaacgtg acgtcaatgg acagtttcgt ggcggaccgg catggtatat ggcgcgcggg
    13861 ctggggatgc gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctatggcata
    13921 attttcagcg gagttcaggc gaacgccgtt gcccgcgccc taagtttttc ttttgatttt
    13981 cctccgctgg tgacaggcat tattctcgct gtctttgctc tgctggcaat cactcgcggt
    14041 cttcatggcg tcgcccggct catgcagggt tttgtcccgt tgatggcgat aatctgggta
    14101 ctgaccagcc tggtgatttg cgtaatgaat atcgggcaac ttccccacgt catttggtct
    14161 atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg gcgcggcggg atatacctta
    14221 agccaggcga ttactaacgg ttttcagcgc agtatgtttt ccaatgaggc gggaatgggt
    14281 tctacaccaa acgcagcagc ggcagcggcg tcctggcctc cgcatccggc agcgcaaggg
    14341 attgtccaga tgattggcat ttttatcgac accctggtca tctgtacggc aagcgccatg
    14401 ctgatattac tggcgggtaa cggcacaacc tacatgccgc tggaaggtat tcagcttatc
    14461 cagaaggcga tgcgggtgct aatgggttcc tggggtgctg agtttgttac cctcgtggtt
    14521 attctgtttg ccttcagctc catcgttgcc aactacattt atgccgaaaa caatctcttc
    14581 tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc ggatctgcac cttcgcaacg
    14641 gtcatcggcg gcaccttgct aagtcttccg ctgatgtggc aactggcaga tatcataatg
    14701 gcctgcatgg ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc
    14761 attgccagtg attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg
    14821 cgttatccgg atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag
    14881 taatcacaac tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg
    14941 caaggactgg atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg
    15001 ttgaccacca tgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat
    15061 gaagcgcgga aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg
    15121 gcgggtatca acgccgctcg ctttcatgac tggcagccag atttcacgcc ggagaatgcc
    15181 cgccaggcga ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc
    15241 agcgaagacg atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc
    15301 gtactgcgcc cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag
    15361 aatgcccgag ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac
    15421 gaggcgctcg cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt
    15481 aaatcggtga agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag
    15541 aagaacggca aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt
    15601 cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa
    15661 ggttacttct ttgatgaaga ttcctccagc aatggcgaac tggtgtttaa acgctacgag
    15721 cagcgttaag tttgtagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa
    15781 gcatgcagat tcaatatatt gcagaattca cgtagatcct gataagacgt gccagcgtcg
    15841 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
    15901 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
    15961 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
    16021 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
    16081 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
    16141 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
    16201 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
    16261 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
    16321 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
    16381 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
    16441 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
    16501 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
    16561 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
    16621 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
    16681 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
    16741 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
    16801 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
    16861 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
    16921 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
    16981 cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
    17041 cgagttagct gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
    17101 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
    17161 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
    17221 cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
    17281 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
    17341 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
    17401 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
    17461 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
    17521 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
    17581 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
    17641 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
    17701 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
    17761 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
    17821 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
    17881 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
    17941 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
    18001 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
    18061 cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
    18121 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
    18181 tgccacgttg tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc
    18241 cagcaaacga gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc
    18301 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt
    18361 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
    18421 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
    18481 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt
    18541 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
    18601 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
    18661 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
    18721 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
    18781 cattaccatc cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa
    18841 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
    18901 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg
    18961 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
    19021 gaatcaatag cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg
    19081 tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact
    19141 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac
    19201 cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
    19261 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
    19321 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt
    19381 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
    19441 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
    19501 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
    19561 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcaaccag ctttgttgac
    19621 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
    19681 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
    19741 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
    19801 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
    19861 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
    19921 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgtacacagt
    19981 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
    20041 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
    20101 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
    20161 ccggtaattc gtcttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
    20221 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
    20281 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
    20341 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
    20401 aaatctcgca acaatcggcg cgtaaacagg cagccaacac cgccgcggag tagtcggaac
    20461 cgttacgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
    20521 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
    20581 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
    20641 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
    20701 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
    20761 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
    20821 ggaaccccgg ctgggcggct gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
    20881 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
    20941 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
    21001 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
    21061 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg
    21121 tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt
    21181 aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt
    21241 aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca
    21301 ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt
    21361 tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac
    21421 ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa
    21481 cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt cgaccagttt
    21541 tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg
    21601 cagttgatgt tggtctgccg ttgtgtcact tttcgtcggt tgttgtatta atgttgctaa
    21661 ttgatagcaa tagaccatca ctgcctgccc cagattgagc gaaggataat ccgccaccat
    21721 cggcacacca gtaagaacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc
    21781 gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac
    21841 cagttctact ggcgtggcgt agtaatgata tttcgcccga ctgcgcgcag tggtggcgac
    21901 agtgaaatcg acatcgtgta acgattcaac cagtgtcggg aaaactttaa tattatcaat
    21961 aatatcacca gatccatgtg cgacccagcg agtggctggc tccagatgtg cctgactatc
    22021 gacaatccgc agttcgctaa accccatcgt tttcattgcc cgcgccgctg ccccaatatt
    22081 ttctgctctg gcgggtgcga ccagaataat cgttatacgc atattgccac tcttcttgac
    22141 caaatgaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt
    22201 cttaacgtaa gtcgcagaaa aagtccttta cttagcttaa aaaaggctaa actatttcct
    22261 gactgtacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt acttaggata
    22321 attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca
    22381 actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc
    22441 aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg
    22501 tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt
    22561 tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt
    22621 gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta
    22681 atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg
    22741 caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa
    22801 cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag
    22861 atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata
    22921 tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg
    22981 ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa
    23041 tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac
    23101 gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg
    23161 aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg
    23221 atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa
    23281 acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga
    23341 aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta
    23401 cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg
    23461 atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta gcgccgtttt
    23521 tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc
    23581 tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc
    23641 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
    23701 cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg
    23761 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
    23821 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
    23881 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
    23941 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
    24001 cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
    24061 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
    24121 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag
    24181 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg
    24241 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
    24301 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
    24361 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
    24421 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
    24481 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
    24541 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
    24601 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
    24661 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    24721 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    24781 ggcacgttcg acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc
    24841 accaaacagg gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    24901 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    24961 aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    25021 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    25081 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    25141 actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt
    25201 tatacgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    25261 atccagtaac gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat
    25321 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac
    25381 atcaacagca gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    25441 ttccaccagt gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac
    25501 tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    25561 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    25621 cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc
    25681 gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat
    25741 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat
    25801 ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt
    25861 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac
    25921 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    25981 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt
    26041 tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat attcattgcg
    26101 cactttgtta atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc
    26161 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    26221 cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    26281 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    26341 cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga
    26401 tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg caacgtttta
    26461 atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc
    26521 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    26581 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    26641 aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc
    26701 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    26761 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    26821 agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata
    26881 tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc
    26941 accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    27001 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat
    27061 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    27121 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    27181 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    27241 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    27301 gaaatggccg catcggaggt atcttccgcc aggcccaatc cccctttaat acccccggtt
    27361 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    27421 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    27481 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    27541 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    27601 tgattaagaa caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta
    27661 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    27721 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    27781 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    27841 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    27901 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    27961 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    28021 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    28081 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    28141 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    28201 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    28261 acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    28321 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    28381 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    28441 aaggtctggg aaccggcgtg caggagttta tcctgacagt atacggaacg tgcatgccaa
    28501 tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    28561 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    28621 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    28681 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    28741 tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccaggc
    28801 atcccagtgc aataccatga ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    28861 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactca ccttcaggaa
    28921 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    28981 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    29041 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    29101 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    29161 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    29221 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    29281 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    29341 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    29401 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag
    29461 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    29521 ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt
    29581 ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc
    29641 ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac
    29701 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    29761 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    29821 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac
    29881 gatgcctgat gcgacgctgc cgcgtcttat cagtatctac aaaatcaatc gcttttcagc
    29941 aacacctctt ccagccactg gcgcagctca acgggcgcgg ctttcaggct gttagatcca
    30001 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg
    30061 ccgcgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
    30121 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
    30181 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
    30241 tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt
    30301 actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt
    30361 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag
    30421 ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca
    30481 cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt
    30541 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
    30601 aactggtgcc aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
    30661 caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc
    30721 cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg
    30781 ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt
    30841 aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa
    30901 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
    30961 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt
    31021 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct
    31081 gcggcgcttt atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg
    31141 ggtagaaacc acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc
    31201 cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca
    31261 tcggcaaacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc
    31321 gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt
    31381 tcgcctgctc gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct
    31441 cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg
    31501 cattttcagc atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa
    31561 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
    31621 aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
    31681 ccaggccagt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
    31741 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcatta ggctggctct
    31801 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
    31861 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
    31921 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
    31981 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
    32041 gattcatcag atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct
    32101 tcagtccagg catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga
    32161 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
    32221 tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa
    32281 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
    32341 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg
    32401 atcgaccaac tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat
    32461 cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt
    32521 cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc
    32581 tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat
    32641 tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat
    32701 tggttacctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga
    32761 agaagcggtt tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta
    32821 cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat
    32881 cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct
    32941 gcgtctgccg gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt
    33001 ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa
    33061 ccacctggat gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac
    33121 cgttgtggcg attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga
    33181 acttgaccgc ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa
    33241 agatcagcgc ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa
    33301 agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct
    33361 ggcacgcttt gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact
    33421 gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa
    33481 atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat
    33541 cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctccgg
    33601 tcaggaacag ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcgtcggt
    33661 tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg
    33721 gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt
    33781 taactttaaa ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg
    33841 tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc
    33901 aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc
    33961 gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat
    34021 cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga
    34081 agagtacaag aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa
    34141 gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac
    34201 aaaccaagaa atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg
    34261 catcaggcat tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca
    34321 gccttagtat ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta
    34381 atctcgacag cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg
    34441 cctttgagcc gtttcatcgc ggttatctct gctcccccat catctcccgc accaactcca
    34501 cgcagcgcag gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt
    34561 tctcttccag catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga
    34621 ggctgcgtaa accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga
    34681 aacggtattc atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc
    34741 agaacgcctg ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca
    34801 ctgcaaaatc aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca
    34861 atgcgatctc atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac
    34921 tggtggtatt gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc
    34981 caccaaggat cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc
    35041 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    35101 gatcgaccag caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt
    35161 ccgaggtgta gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt
    35221 tgctccacac atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    35281 tgcgaatatt tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg
    35341 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    35401 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    35461 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa
    35521 attcaagccc caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt
    35581 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    35641 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    35701 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    35761 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    35821 ccggcgaaaa ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg
    35881 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    35941 acgcaggctg gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt
    36001 cacgccgccg accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg
    36061 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    36121 cggattcgcc atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    36181 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    36241 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    36301 ctcattcacc gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc
    36361 atcaccatcc agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag
    36421 cgagccatct ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    36481 gcgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    36541 atccgccata tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc
    36601 ggcaatcagg cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    36661 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata
    36721 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc
    36781 cgcacctggg ttaccgccaa tcagaatggc gcttcctggc accacgccgc cgcctagtac
    36841 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    36901 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    36961 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    37021 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    37081 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc
    37141 gagcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    37201 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    37261 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    37321 cgccagcggg atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc
    37381 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    37441 ggctaccacg gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc
    37501 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa
    37561 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    37621 cagcgtcgcc acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    37681 ttcggttact tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca
    37741 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    37801 cgggattttc cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg
    37861 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    37921 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    37981 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    38041 accacttaat gaaagaggca gaccaggcca taaagagaca tcttcaggca ggtcgcacca
    38101 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    38161 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    38221 atggctcgca caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt
    38281 gccttgttag tggcgctgat gcagggagcg tcatggttta gtcaaaacca ccagcgacaa
    38341 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtaac gctgaacgtt
    38401 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    38461 ttaacggatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    38521 gcacgttctg gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagcg
    38581 ggcggctatt ttaaccagca gattgtcgaa ccaattgccg ggaaaaacgg accgctcggc
    38641 tatctgcgcc tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    38701 accactaaca ttttacgcct gatgttgctg ctttcactgg caatcggcgt agtgctgacc
    38761 cgcacgctgc tacagggtaa acgtacccgc tggcagcaat cgcccttcct gttaaccgcc
    38821 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    38881 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    38941 aacctggcgg tggaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt
    39001 ctctggcgca atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt
    39061 aatacccggc ggatggaaga agataacgtc cgcctggcac ggcgcagtag cggtggcggc
    39121 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    39181 gataaaacca tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc
    39241 gaagcgtccg ggcgtaacga tctggtggtg aaaaccgccg aaggtgaccg caaagtctca
    39301 ggatcggcct atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat
    39361 gccgacctta gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    39421 ggcattacct cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc
    39481 catgagcagg tttgcgaagc cataaccaag gcctttttcg cccattatgg cgagcgtgta
    39541 gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt
    39601 gcccgtcaga gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg
    39661 gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc
    39721 acccgcgccc aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg
    39781 cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg
    39841 gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg
    39901 gtaaggtaac tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca
    39961 ggagcgggag attatcaatt taatgttaag ccagcgtatt tcctggctag ccgccgtgcg
    40021 tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc
    40081 tctaaaatag atcgaaaaga cggaacggtt tagctcaaac atcaaaagca aaatggcaga
    40141 aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa
    40201 atgaatagat tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata
    40261 atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct
    40321 cccactccgg cttcacacga caatcgcttt tcagcgaaac acaattactc tttatcgccc
    40381 agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg
    40441 gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc
    40501 gcgccaaatt ctgcagcgac gccgtagata ccagctgctt ccatttccac gccgaggatg
    40561 ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag
    40621 aacaggttac caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgt
    40681 accatgtcaa agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact
    40741 ttggaatcgg tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga
    40801 actgcgccac aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct
    40861 ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg
    40921 cctttgtaag taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct
    40981 tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg
    41041 tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca
    41101 acatgttttc cccctctccc tgtgggagag ggccggggtg agggcatcaa gccgcaccca
    41161 ttatcagaac atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt
    41221 ctggccgata tccgcgaagg tttcacggtg acccagtgaa cccggtttta ctttcgggcc
    41281 gtaaaccagt accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca
    41341 accgtggtca gcagtgagga tcaggatgtc gtcatcgcgc agcaaagaca tcagctccgg
    41401 cagacggcgg tcgaacagct ccagacccgc agcataaccg gcgacgtcgc gacggtggcc
    41461 ccaggaagag tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt
    41521 catctctttg atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt
    41581 gataccgcag ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt
    41641 ttcatcaacc agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg
    41701 gttaccggta cgctggaagt taccggcttt gtcaccgata aacggacgag cgataacacg
    41761 accgatgttg tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcatagag
    41821 tttatccaga ccgaaagttt cttcatggca ggcaatctgg aacacggagt cagcggaggt
    41881 atagaaaatc ggcttgccgg ttttcatgtg ctcttcgccc agttgatcca gaatgaccgt
    41941 accggaagag tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc
    42001 cagcagctct tgcgggaagc tgttttcgtg atcggagaaa tatccccact caaacagaac
    42061 cgggacaccg gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg
    42121 cgcccatgcg tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc
    42181 ggtagaacct tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg
    42241 gcctttacga ccgttatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt
    42301 gtcagccccg acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc
    42361 cagcaccata ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat
    42421 gatcagatca gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg
    42481 tgctttatcg gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt
    42541 ttcgtcttta gcgtggataa ccgccagcgg acgctgaccg tctacctggt cgcccaaacg
    42601 cgccatatca gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc
    42661 gcccattgca accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc
    42721 ggtatcagca tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc
    42781 aacgaagtcg gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt
    42841 accgttgtcc agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt
    42901 gccggagatc agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg
    42961 atattcaccc gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga
    43021 ggccagtacc tgattcatat cggtgagcag cgcggtggtg cgcacgccag cgccgttagc
    43081 cacgccaaca atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc
    43141 gctacccact ttcacgtcca tcaccagcgc gtccagacct tccgccagtt tcttcgccag
    43201 gatcgaggca gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta
    43261 gaaacgttta tccgccggag ccagtgagct ggtctggccg ataatcgcta cgccgacgtc
    43321 tttaataatt tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc
    43381 cagtttgtcg agcgtaccgc cagtatgacc gaggccgcga ccagagatca tcggaatata
    43441 gccgccgcag gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc
    43501 ggtggagtgt ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt
    43561 tcctgaatct cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa
    43621 gaaaatggtc atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatgcc
    43681 gttgataaag aaacgaattt cttcatcgct cagcgcatga ccatcacgtt ttttacgaat
    43741 aatttcttgt gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc
    43801 cggatgcgtc tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg
    43861 cagcgcatca ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg
    43921 gcccagcgct ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc
    43981 atctgcccag tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc
    44041 cgcagtacgc acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg
    44101 gatcacttcc atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt
    44161 agaggttttg atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag
    44221 cgcttcgtct ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc
    44281 gcaagcctct ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ctgccatcag
    44341 cgcgcggtac gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt
    44401 ttctgccagc gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat
    44461 tcggatttcc ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg
    44521 atagatacag atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat
    44581 tactttctcg tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg
    44641 caggctgctt gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca
    44701 ccttgcgagt ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca
    44761 attaaggaaa tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca
    44821 ctttcaaatg aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa
    44881 cttcttacaa tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg
    44941 aaaatgcact gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc
    45001 agaagcgcca ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg
    45061 ccgaagcccg ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc
    45121 cgtacaaacc gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat
    45181 ggctggagct ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt
    45241 accaccacgt accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt
    45301 tgcaaccata tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct
    45361 ggcgttacct cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg
    45421 attcgcccat tacccacgcg atcttacgtg cagacgcaga tttgaagcag gtttcaccga
    45481 acctgacctt tatctacgat cctgaaatca cccctgatga cctgctgctg aaagtggcga
    45541 agaacatctg tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaatttt
    45601 tcacaaaagg gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg
    45661 gcagcacgct ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg
    45721 acttctttac gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc
    45781 ggtgtgaatt cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag
    45841 ggctgattaa ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg
    45901 ttaacttgct gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa
    45961 atgaagtggg ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga
    46021 aatatggctg gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccaatatcg
    46081 gcaccacgcc gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc
    46141 aaactgtcgc cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg
    46201 acgaaaccat caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag
    46261 ccggaatgcg tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt
    46321 atatggtgcg tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca
    46381 cctggctggg cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga
    46441 taagccatga acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac
    46501 gggccaggca gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt
    46561 cacaatccgt ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg
    46621 catcaggcgt tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ttgcgagcag
    46681 tgtgatacct gcctgaagag gtgtccgcaa catgccacgc caatggcgca atccatgagc
    46741 gtggatgagg tgcttagcca tatccgcaaa gcggtgctgt ttattgaagg gataacggta
    46801 agcggcggcg aagccacgac ccaactgccg tttgtagtgg cgctatttac agccatcaaa
    46861 aacgatccgc aactgcgcca cctcacctgc ctcgtggaca gtaacggtat gttgagcgaa
    46921 accggctggg aaaaattact cccggtgtgc gacggcgcga tgctcgatct caaagcgtgg
    46981 gggagcgaat gtcatcagca attaaccgga cgcgataatc agcagattaa gcgcagcatc
    47041 tgtttgctgg cagagcgcgg taagctggcg gaactgcgtt tgctggtaat tccagaccag
    47101 gtggattatt tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt
    47161 ccggttcgcc tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg
    47221 agcgccacgc cggaaggcgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc
    47281 cggttgatat ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca
    47341 aacctgtagg catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg
    47401 gatgctgcct acgaagggta aactacccac gaacgctaaa caccgcatac gtattattaa
    47461 gcaacacttc ggcaatctca tcttctggtt ccggacgtaa ctcgcaaagc acgtcaaaca
    47521 cccgcgcagc ctgttccggg cggttaggct tcccctgaaa gccgttgagc ggcatatccg
    47581 gcgcgtcggt ttccagcaac aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt
    47641 tactggcgcg tggataggtg atagtaccgc ctacgccgat tttgtagccc agctgtacaa
    47701 accgctcggc ctgttgcagg ctgccggaaa aaccgtgaac cacgccagtg cgcgataaat
    47761 cgtggcgttt aagatgcatc gccagtttgt cgtgtgtgcg ccgtgaatgc aggatcaccg
    47821 gcagatcgta gcgtttcgcc agtttcagct gttcgtcgag taaccactgt tgcctctcaa
    47881 attgcggatc gtcgccgaag agatccagac cgatctcccc caccgccacc accttcgccg
    47941 gacgcctttc cagcgcctgc tgtagctgat caagagacac atcgctatgt ttttccaaca
    48001 tccccggatg caaccccaat gcggcataca gcggttgata tttttccgct aacgcctgca
    48061 cgcgggcaaa attttccgcc tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg
    48121 ccgcgcgttg caggctggcc tcttcatcgc cactaaacgg cgggaaatca aaatggcagt
    48181 gggtatcgat aaaacggcaa atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc
    48241 ctgcggagca tcgctcactt cggcaaccag cgtatcgttg gcgactggcg caggcggaat
    48301 aacgatcgat tccggcgtca ccactggcac cagatggcgg gtaagcggcg ctttttcagt
    48361 taatagcttg ccaaccgtgg cgaggaaata acgaccgcat aaacgcccaa gtttatagtc
    48421 ttcacgcagc gccggaatcc gactgccaag cgcgatacta tgtaatggct tcggtggata
    48481 aatttcgaat atccgcagct tgcccggtgg tttctcaata aattgctgaa tgtcacgata
    48541 gctggtttca tgatgctgca ccagattgac cagcggctgc aggctactgt cacccagcca
    48601 gcgttccatg cgtttgaacc actgcggcgt gtagtacatt tgtgacggca cagtgcggat
    48661 gacgaccaac gttttagcgc cctgccttgc cgcctcttta accggaatcg catcactgat
    48721 cccgccatcc aggtagttaa tgccttccag cgacactccg ctacgataaa agccaggtat
    48781 cgcactggag gcgcgaatca catccagcca gttttgtttg gttggtaaaa agtaattagg
    48841 cgcgtagtca tcctgacgac aggcgcacat ataaaacgat ttgccgctgt caaacaaccg
    48901 cgcggcggta tccatttgca acggcatctg gcttgcggtg gcctccacca gccagtcgag
    48961 atcgataaga tttcctccac ggacaaagcg caatggatcg aaaaattcgc gttttgtggt
    49021 atagcgcatg atgactttgc gcgcgtaacc gggctgattg cagataaacg ccgagaggtt
    49081 ctgcgccccg gcagatgtgc cgagataaag atcgaaagga ttaaactgcg cgcgcataaa
    49141 ctcatccagc acgccagccg tgaaaattcc acgctgtccg ccgccttcgc acaccagagc
    49201 tattcgtcca ggctggaacg gccttagcga caacggcgca atattaccaa gcgttacagg
    49261 tattcgctgc cccacctctg ccttcctgtt tttgttgtga aagaatgaca aagtaacgca
    49321 atttattatt cgctaaaacc gcaaaagcca gtccgctgga ctggctttgg ataaaatttt
    49381 cttaatggtc tgaaatctag ggtcgttttc ggcccatgaa caaactcacc aggaacagaa
    49441 taatcccgac gacaaagaca attttagctg cgcctgcagc ggtaccggcc agaccaccaa
    49501 acccaagtgc ggcggcgatt aacgcgataa ccagaaatat gatgccccaa cgaaacataa
    49561 gcgtctcctt taccatagtt aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc
    49621 gatattttta gtgtggtgca catcgcgcct cccgacaaag ttcgggagga cgaattacga
    49681 cgaattactt agttttcaga tcatttttaa cgcttttcac accatctacc gctttggcga
    49741 tactttcagc acggtcgctc tgtgcctggg aatcgacggt accggagagc tgaaccacgc
    49801 cgtcggtggt ttcaactttc acatgacggg aagggacgat atcgtccgcc agcagtttgg
    49861 ctttgatttc actggtggtg gcggtgtcac ccgcgtagcc cttcacagag ccttctttag
    49921 cgtcgcgaac gtgcagtttg tcgctgacag aggtcacccc ttcaacgcct ttcgccactt
    49981 tcactgcctc ttcggcctga gcctggcttt caacgaaacc gctcagggtc acgacttttt
    50041 gatcggtttt gacagagata tcggtgctct tgatgttgtc atgatccacc agcgccgcct
    50101 tcactttcgc ggtgatggcg ctgtcatcca tgaaattacc gactttattc atagagctat
    50161 cgactttttg ccctgcgctt tcattggtag tctgtgcgtt gttttccgcg taggcagagc
    50221 cggtcgcgac ggcagaggtc aacattacag ccagcagagt tttcgaaatc ttcagtcttg
    50281 tcatagtcat cgatttattc ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt
    50341 tgcattgctg aatagggaga gacttcaccc tctacagaag tcaatggtcg ccatcacaaa
    50401 agcgatgggt gatgaataac gaccattaca gcctctgaat cagttattaa tatcggtaga
    50461 atgacaatcg acggctttag atactgatat ctacgcattg aacggtattt aacgccgtca
    50521 gaaatgtcat tactttgtta aatatagatc acaattttga aaccgctcgt gatatcagcg
    50581 aaaacataag caaaagtgaa tgttttaaga acattccgta agcggctaat aaggaaggga
    50641 aattgacagg gcgcagcggc taccgcgccc tggaggcaag aattaatgct cgcgggtctg
    50701 gtggaactga acgtccggat aacgttcctg cgccaggcgc aggttgacca tgctggtagc
    50761 gatgtaagcg aggttatcgc cgccatcaag cgccagttgg ctttcgttct tacgcttgaa
    50821 ctcttcgaat ttcttcgcgt ccgcacattc tacccagcgg gcagtggcaa cgttgactga
    50881 ttcatatact gcttcaacgt tgtattcgct cttcaggcgc gataccacca catcaaactg
    50941 cagcacacca acagcaccaa cgatcaaatc gttgttggag atcggacgga acacctgcac
    51001 cgcgccctct tcggaaagct gtaccagccc tttgagcagc tgtttttgct tcagcggatc
    51061 tttcaggcgg atacgacgga acagttctgg tgcgaagttc ggaataccgg tgaacttcat
    51121 catctcaccc tgggtaaagg tgtcgccgat ctgaatagtg ccgtggttgt gcaggccgag
    51181 gatatcgccc ggatacgctt cttcaacgtg cgaacggtcg cccgccataa aggtcagcgc
    51241 gtcggagata accacatctt tcgcagtacg cacctggcgc agcttcatgc ctttttcata
    51301 tttaccggat actacgcgca taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc
    51361 ctgaatttta aatacgaagc cggtaaattt gtcttcgctc gcctctacgg tacgggtatc
    51421 agtctgacgc ggcatcggcg caggagccca ctccaccagg ccatccagca tatgatcaac
    51481 gccgaagtta cccagcgcag taccgaagaa taccggagtg atttcgcccg caaggaacaa
    51541 ttctttgtcg aactcgttag acgcgccttt caccagttcc agttcgtcac gtagctgctg
    51601 tgccagacct tcaccaaccg cagcgtcgag atccgggtta ttcagccctt taacaatacg
    51661 gacttcctga atcgtgtggc ctttaccgct ctgatagaga taggtttcgt ctttataaag
    51721 gtggtaaacg cctttaaaca gcttgccgca gccaatcggc caggtgatcg gtgcgcagcc
    51781 gattttcagc tcgttctcaa cttcatcgag cagttccatc gggtcgcgga tatcacggtc
    51841 aagtttgttc ataaaggtga ggatcggcgt gtcgcgcaga cgggtaactt ccatcagctt
    51901 acgggtacga tcttcaacac cttttgcagc gtcgataacc atcaggcagc agtccaccgc
    51961 cgtcagggta cgataggtat cttccgagaa gtcttcgtgc cccggggtgt cgagcaggtt
    52021 aaccaggcaa tcgtgatacg gaaactgcat cacagacgta gtaatggaga tcccacgctg
    52081 cttttccatc tccatccagt ccgacttagc gtgctggttg gaaccacggc cttttactgt
    52141 accagcggtc tgaatggcct gtccgaacag cagcaccttc tcggtgatgg tagttttacc
    52201 ggcgtccggg tgagaaataa ttgcaaaggt tcttcttttc gctacctctg cggccaaaag
    52261 gcttgtcata attgttgtct ctttgattat gtacaatcat atgtacaaag aaaaattcat
    52321 gccgctgatt gtacatttta gaggtatcag agcatagcag aacatgatca gtaggtgtat
    52381 ttgtagccaa agaacatttg tacatgcagc aatctttgtt ctattgacac tgtttattca
    52441 tacagttaaa aataacactg tatacaaaca cagtgtagag ggatttttat gcgtattgag
    52501 atctgcatag ccaaagagaa aatgactaaa atgccaaacg gtgctgtgga tgcgttaaag
    52561 gaagaattaa ctcggcgcat cagtaagcgt tatgacgatg tagaggtgat cgtaaaagcc
    52621 accagcaacg atggcctttc tgttacgcgc accgccgata aagattcagc taaaactttt
    52681 gttcaggaaa ctctgaaaga tacctgggag tctgctgacg agtggtttgt tcactaatta
    52741 acacgtaaaa tcggtaacgg ctggaaatca ttcaatactc gcactatcga aagttcgcca
    52801 gccagccgtg gcacgttctt gcatacgacg tgccgcggat cctattattc tagtatgaaa
    52861 tccctaaccg atttcaaatt ctccagactg ctcagatt
//
LOCUS       DAZUFB010000022        52835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZUFB010000022 DAZUFB010000000
VERSION     DAZUFB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.022"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            194..1168
                     /gene="hemB"
                     /locus_tag="ACHY3M_001511"
     CDS             194..1168
                     /gene="hemB"
                     /locus_tag="ACHY3M_001511"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFS4316449.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1273..2124)
                     /gene="tauD"
                     /locus_tag="ACHY3M_001512"
     CDS             complement(1273..2124)
                     /gene="tauD"
                     /locus_tag="ACHY3M_001512"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFS4316450.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2121..2948)
                     /gene="tauC"
                     /locus_tag="ACHY3M_001513"
                     /pseudo
     CDS             complement(2121..2948)
                     /gene="tauC"
                     /locus_tag="ACHY3M_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
     gene            complement(2945..3712)
                     /gene="tauB"
                     /locus_tag="ACHY3M_001514"
     CDS             complement(2945..3712)
                     /gene="tauB"
                     /locus_tag="ACHY3M_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFS4316451.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3725..4687)
                     /gene="tauA"
                     /locus_tag="ACHY3M_001515"
     CDS             complement(3725..4687)
                     /gene="tauA"
                     /locus_tag="ACHY3M_001515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4316452.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5303..5974
                     /locus_tag="ACHY3M_001516"
     CDS             5303..5974
                     /locus_tag="ACHY3M_001516"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFS4316453.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            5984..7180
                     /gene="yaiP"
                     /locus_tag="ACHY3M_001517"
     CDS             5984..7180
                     /gene="yaiP"
                     /locus_tag="ACHY3M_001517"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4316454.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDHFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            7158..7739
                     /locus_tag="ACHY3M_001518"
     CDS             7158..7739
                     /locus_tag="ACHY3M_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4316455.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            7741..8514
                     /gene="yaiO"
                     /locus_tag="ACHY3M_001519"
     CDS             7741..8514
                     /gene="yaiO"
                     /locus_tag="ACHY3M_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFS4316456.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            8699..8974
                     /gene="frmR"
                     /locus_tag="ACHY3M_001520"
     CDS             8699..8974
                     /gene="frmR"
                     /locus_tag="ACHY3M_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFS4316457.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            9009..10118
                     /gene="frmA"
                     /locus_tag="ACHY3M_001521"
     CDS             9009..10118
                     /gene="frmA"
                     /locus_tag="ACHY3M_001521"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFS4316458.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10211..11044
                     /gene="fghA"
                     /locus_tag="ACHY3M_001522"
     CDS             10211..11044
                     /gene="fghA"
                     /locus_tag="ACHY3M_001522"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS4316459.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11169..11708)
                     /gene="yaiL"
                     /locus_tag="ACHY3M_001523"
     CDS             complement(11169..11708)
                     /gene="yaiL"
                     /locus_tag="ACHY3M_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFS4316460.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(11810..13021)
                     /gene="mhpT"
                     /locus_tag="ACHY3M_001524"
     CDS             complement(11810..13021)
                     /gene="mhpT"
                     /locus_tag="ACHY3M_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFS4316461.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASALGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(13296..14309)
                     /gene="mhpE"
                     /locus_tag="ACHY3M_001525"
     CDS             complement(13296..14309)
                     /gene="mhpE"
                     /locus_tag="ACHY3M_001525"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFS4316462.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(14306..15256)
                     /gene="mhpF"
                     /locus_tag="ACHY3M_001526"
     CDS             complement(14306..15256)
                     /gene="mhpF"
                     /locus_tag="ACHY3M_001526"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFS4316463.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(15253..16062)
                     /gene="mhpD"
                     /locus_tag="ACHY3M_001527"
     CDS             complement(15253..16062)
                     /gene="mhpD"
                     /locus_tag="ACHY3M_001527"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFS4316464.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(16072..16938)
                     /gene="mhpC"
                     /locus_tag="ACHY3M_001528"
     CDS             complement(16072..16938)
                     /gene="mhpC"
                     /locus_tag="ACHY3M_001528"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFS4316465.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(16956..17900)
                     /gene="mhpB"
                     /locus_tag="ACHY3M_001529"
     CDS             complement(16956..17900)
                     /gene="mhpB"
                     /locus_tag="ACHY3M_001529"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFS4316466.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(17902..19566)
                     /gene="mhpA"
                     /locus_tag="ACHY3M_001530"
     CDS             complement(17902..19566)
                     /gene="mhpA"
                     /locus_tag="ACHY3M_001530"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFS4316467.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            19757..20590
                     /gene="mhpR"
                     /locus_tag="ACHY3M_001531"
     CDS             19757..20590
                     /gene="mhpR"
                     /locus_tag="ACHY3M_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFS4316468.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            20667..21749
                     /gene="lacI"
                     /locus_tag="ACHY3M_001532"
     CDS             20667..21749
                     /gene="lacI"
                     /locus_tag="ACHY3M_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFS4316469.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            21872..24946
                     /gene="lacZ"
                     /locus_tag="ACHY3M_001533"
     CDS             21872..24946
                     /gene="lacZ"
                     /locus_tag="ACHY3M_001533"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFS4316470.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVIPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            24998..26251
                     /gene="lacY"
                     /locus_tag="ACHY3M_001534"
     CDS             24998..26251
                     /gene="lacY"
                     /locus_tag="ACHY3M_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFS4316471.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(26330..27511)
                     /gene="cynX"
                     /locus_tag="ACHY3M_001535"
     CDS             complement(26330..27511)
                     /gene="cynX"
                     /locus_tag="ACHY3M_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFS4316472.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(27517..27987)
                     /gene="cynS"
                     /locus_tag="ACHY3M_001536"
     CDS             complement(27517..27987)
                     /gene="cynS"
                     /locus_tag="ACHY3M_001536"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFS4316473.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(28018..28677)
                     /gene="cynT"
                     /locus_tag="ACHY3M_001537"
     CDS             complement(28018..28677)
                     /gene="cynT"
                     /locus_tag="ACHY3M_001537"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFS4316474.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            28787..29686
                     /gene="cynR"
                     /locus_tag="ACHY3M_001538"
     CDS             28787..29686
                     /gene="cynR"
                     /locus_tag="ACHY3M_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFS4316475.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(29726..31009)
                     /gene="codA"
                     /locus_tag="ACHY3M_001539"
     CDS             complement(29726..31009)
                     /gene="codA"
                     /locus_tag="ACHY3M_001539"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFS4316476.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(30999..32258)
                     /gene="codB"
                     /locus_tag="ACHY3M_001540"
     CDS             complement(30999..32258)
                     /gene="codB"
                     /locus_tag="ACHY3M_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFS4316477.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(32597..34483)
                     /gene="prpE"
                     /locus_tag="ACHY3M_001541"
     CDS             complement(32597..34483)
                     /gene="prpE"
                     /locus_tag="ACHY3M_001541"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFS4316478.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(34523..35974)
                     /gene="prpD"
                     /locus_tag="ACHY3M_001542"
     CDS             complement(34523..35974)
                     /gene="prpD"
                     /locus_tag="ACHY3M_001542"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFS4316479.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(36008..37177)
                     /gene="prpC"
                     /locus_tag="ACHY3M_001543"
     CDS             complement(36008..37177)
                     /gene="prpC"
                     /locus_tag="ACHY3M_001543"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFS4316480.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(37222..38112)
                     /gene="prpB"
                     /locus_tag="ACHY3M_001544"
     CDS             complement(37222..38112)
                     /gene="prpB"
                     /locus_tag="ACHY3M_001544"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFS4316481.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            38351..39937
                     /gene="prpR"
                     /locus_tag="ACHY3M_001545"
     CDS             38351..39937
                     /gene="prpR"
                     /locus_tag="ACHY3M_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFS4316482.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(40035..40310)
                     /gene="yahO"
                     /locus_tag="ACHY3M_001546"
     CDS             complement(40035..40310)
                     /gene="yahO"
                     /locus_tag="ACHY3M_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFS4316483.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            40457..41128
                     /gene="yahN"
                     /locus_tag="ACHY3M_001547"
     CDS             40457..41128
                     /gene="yahN"
                     /locus_tag="ACHY3M_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFS4316484.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(41145..41390)
                     /gene="yahM"
                     /locus_tag="ACHY3M_001548"
     CDS             complement(41145..41390)
                     /gene="yahM"
                     /locus_tag="ACHY3M_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFS4316485.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(41802..42617)
                     /gene="yahL"
                     /locus_tag="ACHY3M_001549"
     CDS             complement(41802..42617)
                     /gene="yahL"
                     /locus_tag="ACHY3M_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFS4316486.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(42860..43909)
                     /gene="yahK"
                     /locus_tag="ACHY3M_001550"
     CDS             complement(42860..43909)
                     /gene="yahK"
                     /locus_tag="ACHY3M_001550"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFS4316487.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAEPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(43996..44952)
                     /locus_tag="ACHY3M_001551"
     CDS             complement(43996..44952)
                     /locus_tag="ACHY3M_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4316488.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(44949..45920)
                     /locus_tag="ACHY3M_001552"
     CDS             complement(44949..45920)
                     /locus_tag="ACHY3M_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4316489.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(45913..47397)
                     /locus_tag="ACHY3M_001553"
     CDS             complement(45913..47397)
                     /locus_tag="ACHY3M_001553"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS4316490.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(47446..48429)
                     /locus_tag="ACHY3M_001554"
     CDS             complement(47446..48429)
                     /locus_tag="ACHY3M_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4316491.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(48683..49093)
                     /locus_tag="ACHY3M_001555"
     CDS             complement(48683..49093)
                     /locus_tag="ACHY3M_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4316492.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(49392..50774)
                     /gene="yahJ"
                     /locus_tag="ACHY3M_001556"
     CDS             complement(49392..50774)
                     /gene="yahJ"
                     /locus_tag="ACHY3M_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4316493.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(50784..51314)
                     /locus_tag="ACHY3M_001557"
                     /pseudo
     CDS             complement(50784..51314)
                     /locus_tag="ACHY3M_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(51620..52126)
                     /locus_tag="ACHY3M_001558"
     CDS             complement(51620..52126)
                     /locus_tag="ACHY3M_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4316494.1"
                     /translation="MILDQEWILASDLLGVGGLPSSLAGLHKRAKNEGWEKRSVTTPG
                     IRGRAFAYRLSDLPAHVVSIITGSAEIKHQPPQKNGGNSELLELIDTLSQEEQELVIY
                     TLRRKGVEKLLEFCNQENQELIALTGIRRLAALSLNNLSDQKVREIFEGDETKDHHFN
                     LTHKEAKA"
     gene            52356..52601
                     /locus_tag="ACHY3M_001559"
     CDS             52356..52601
                     /locus_tag="ACHY3M_001559"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4316495.1"
                     /translation="MSLIEDWSSADIKAALEKRDTNLSRLSRENNLQPRTLGNALRVP
                     YPKGEKIIARAIGVEPQVIWPTRYERRARRKYVGCND"
     gene            52582..>52835
                     /locus_tag="ACHY3M_001560"
     CDS             52582..>52835
                     /locus_tag="ACHY3M_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4316496.1"
                     /translation="MWVAMTELISFSDLPSSLAGLHKKAKREAWKTRLKPGVKGKVLE
                     CEIGALPLTVQQAVRERYALQLMTQKADESPAPVVTKARRS"
BASE COUNT        12743 a        14286 c        13302 g        12504 t
ORIGIN      
        1 ataataacta agagctataa ataaagatta aaaatatttt cagtgaatat gaaattattg
       61 ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc aacatcacga
      121 caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta attccacaaa
      181 catcaggcag accatgacag acttaatcca acgccctcgt cgcctgcgca aatctcctgc
      241 gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt tgccgatctt
      301 tgttgaagaa gaaattgacg actacaaagc cgttgaagcc atgccaggcg tgatgcgcat
      361 tccagagaaa catctggcac gcgaaattga acgcatcgcc aacgccggta tccgttccgt
      421 aatgactttt ggcatctctc accataccga tgaaaccggc agcgacgcct ggcgggaaga
      481 tggactggtg gcgcgtatgt cgcgcatctg caagcagact gtgccagaaa tgatcgtcat
      541 gtcagacact tgtttctgtg aatacacttc tcacggtcac tgcggtgtgc tgtgcgagca
      601 tggcgtcgac aacgacgcaa ctctggaaaa tttaggcaag caagccgtgg ttgcagctgc
      661 tgcaggcgca gacttcatcg ccccttccgc cgcgatggat ggccaggtac aggctattcg
      721 ccaggcgctg gacgctgcag gctttaaaga tacggcgatt atgtcgtatt cgaccaagtt
      781 cgcctcttcc ttttatggtc cgttccgtga agctgccgga agcgcattaa aaggcgaccg
      841 caaaagctat cagatgaacc caatgaaccg tcgcgaggcg attcgtgaat cactgctgga
      901 tgaagcccag ggcgcagact gcctgatggt taaacctgct ggagcatacc tcgacatcgt
      961 gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg tatcaggtga gcggtgagta
     1021 cgcgatgatt aagttcgccg cgctggcggg tgctatagat gaagagaaag tcgtgctcga
     1081 aagcttaggt tcgattaagc gtgcgggtgc ggatctgatt ttcagctact ttgcgctgga
     1141 tttggctgag aagaagattc tgcgttaatt tttttcctct cagaaagggg gagttaacct
     1201 gttgaaagta cggtgaacgc cccctcaccc taaccctctc cccaaggggg cgagggaacc
     1261 gtcccactcg tatcaccccg cccgataaaa cggtttatcc ccaaggatcg tcgcccgatg
     1321 cataatccgc cgctgtggca ggtaatcggc attggcatag tgctgagtca cgcggttatc
     1381 ccaaatcgca atatcattcg gttgccagcg ccaacgcacc tgaaactccg gtttagtgat
     1441 atgggcgaat aaaaagccta acaaggcttc gctctctttc tcgctgacat cgacaattcg
     1501 cgtagtaaag ccttcattca caaacagcgc ctgtttaccg ctcaccggat gcgttcgcac
     1561 caccggatgt agcaacggag gatttttcgc gaccgcctcg cgccagcgtt gatgttcctc
     1621 ctcggttttg cggtatttgt attccgggaa cgatttacgg aaatcatgct ccgcacgcag
     1681 cccgctcagc agctggcgga agggaacaga gagcgcctca taggccgcaa taccgctggt
     1741 ccagagcgta tcaccgccgg tcgaaggtaa ctctttagct gccagaatcg cccctgcggg
     1801 tggcgtttca ataaatgtca catcggtatg ccagttgtcg ttatccggcg gattatcgtt
     1861 atgggtatcc agcacgatga tctcgtcaac cccttcggca tgagggtaaa cagggtgaat
     1921 atgcagttcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgcaa tagtttgatc
     1981 gcgcagaaac accacctgat ggcgcagcac cgcatggtaa agctgttcaa actgattatc
     2041 gcttaacggg cgcgtcaggt cggcacccga aatttgtgcg ccgatatacg gccccagcgg
     2101 ggtaatgctc agacgttcac tcattgtact tctccatgcc agggcgtcag acggcgctgt
     2161 aacgcgcgca aacccagttc taaaagaaag gcgataatcg caatcaccgc gatccccgcc
     2221 agcaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc taaaccgcgc
     2281 gtcgcggcaa tcagctccgc cgctaccagc gtagaccacc ccacacccag accaatgcgt
     2341 aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag cacctgcgca
     2401 cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct tttcaccccc
     2461 gccagcgccg acatcgccac cggtgcaaaa atcgctaaat agatcagtaa gatcttcgag
     2521 gtttcaccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg cggcaccgga
     2581 cgataaagtt cgattaccgg atccagaatg ccgcgcaccg tcgggcttag ccccatggcg
     2641 atcccgaccg gaataccgat caccaccgcc gcgagtagcg ccagcacaat gcgcgtcagg
     2701 ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg
     2761 agcagttttg ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat cagttgcagc
     2821 gccgccaccg tccaccatac agtgagtaaa accgctaacg tgccaatgct taaggtcacc
     2881 tgacgctaga gcggccagcg ccatttcagc cgccgcgaat gcagtttttc attaatgagc
     2941 acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat tcgcgcatgg
     3001 cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca aagcggcgag
     3061 caaagttgag cggcagccgc tccagcacgc ggccagggcc gggtgaaagc agaaccagtt
     3121 cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc tgcttgcccg
     3181 tctcctgcca gagtttcagc agcagggttt gcatctggtc gcgggtgaag gcgtccagcg
     3241 caccaaacgg ctcgtcgagt aataacagct ggggattcgc cgccagcgcg cgggcaatcc
     3301 ccacccgctg acgttgacca ccggaaagct gccagatata gcgtttttct gcgccttcca
     3361 gccccacttt tttcagcatc tggtgcgcga tttccagtcg ctgcattttc tctatacctg
     3421 ccaattgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtagc aacccttcat
     3481 tctgaaaaac caccccgcgc tctgctcccg gtccctcaat acgcttaccc gccagttgaa
     3541 tgctgccatg ctgataaggc acaaaaccgg caatcagatt cagcagggtg gttttaccgc
     3601 agccggacgg ccccagcacc accagtagct cgccgctttc cagcgtcagg ttgatatctt
     3661 ccagtgccgg tttgccgcca taatcggcgt aaagatgaga gatttgcagc atctgcgcct
     3721 ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct acagccggga
     3781 ccttgccctg ctctttcaaa aactgcgcgg tgtcgatgat cgctttgttc accggtccgg
     3841 tcagttctgc cgtttgttgc tgcggcgtca gataggtatt ccccttcacc agccccggaa
     3901 cgtcaccttc cggcacgccg cttaaacgcg ccagtttgct gatgttttcc ggctgtttca
     3961 gccacgcgtc tgggttagca atgtacggtt gctgagcatc gatggcgctt ttagcgaacg
     4021 ctttcacgac ctcaggatgt ttctcggcaa aatctttgcg caccacccag acgtccagcg
     4081 ttggcgcacc ccactgcccg acctgttcag aatcggtcag caccttgccg tctttttcca
     4141 gggcgttaac cgccggtgcc cagacataag caccatcaat atctccccgc tgccaggcag
     4201 cgataatcgc gggcggctgc aggttcacaa tctccacttg cccgggttta atgccccagt
     4261 gtttcagcgc cgccagcagg ctgtagtggg tggtagagat aaacggtacg gcgatgcgtt
     4321 tgccaatcag atcttccggt ttgctgatag ttttctttac caccagcgct tcggagttac
     4381 ccagttttga cgccagcaag aaaacttcaa tcggcacctg ttggctggct gcaaccgcta
     4441 acgggctgga accgaggttg ccgatttgca catcgccaga agccagcgcc cgcacgatgc
     4501 tggctccgct gtcaaactta cgccagtcca cggttgctcc gctttcttta gcaaaggtgt
     4561 tgtcggcctg agccactttc gccggttcgg ctgaggtttg atacgcaacg gtgacgttca
     4621 ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta cgcgatgaaa
     4681 ttgccatgat tgtctgctcc cctgtcttgt tatgtgagca gtattcagga ataaaaacat
     4741 tcattaaaag aattagtcgt tatcgcacag atgattttat tcttagcaaa aaaacggcaa
     4801 aggaaagatt aaatatgcaa ttactccgtt ctaacaattg ggaaaattac tacacttcat
     4861 tttagtgatg cataaacaac tacattacga gatttattac ttcattattt ctatcaatat
     4921 caatacatat aataaaacta ttatattcgc attgtatata tcactgtatc attctcaatc
     4981 actcgcatat tacattcaca taatgtatat aaacaatact tgttcacaga acatattttt
     5041 taaatgcaat taattgacaa actaaatcac atctaaatac aatgtataaa acaaaataac
     5101 tatacataca aaacatacaa acacttaaca tatgaattgc ataaaaacat tacccagtcg
     5161 aacctccggg tctgaaagcc cttgcttttc agttattagc aacatttaac atttataaat
     5221 taacatctgg ctgcgccaga gattacgtct ctgagtgcat cgttgtgctt gtagtataat
     5281 aaataaaatg gagtgtcatt aagtggataa ggttttagat tcagccctcc cttcttcggc
     5341 aaataaaaga aagggtatcc ttgctattgg cgcacatcct gacgatatag aattaggctg
     5401 tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat atcgcagccg tggtgatgac
     5461 taccggcaac tctggtacag atggaataat cgatcgccat gaagaatcgc gcaacgccct
     5521 aaagatatta gggtgccacc aaactattca tcttaatttt gctgacaccc gcgctcattt
     5581 acagctcaat gatatgattt ccgctctgga agacatcatt aaaaatcaaa ttccttctga
     5641 tgttgaaatc atgcgggtat ataccatgca tgatgccgac cgccatcagg atcatctcgc
     5701 tgtttatcaa gcttcaatgg ttgcctgccg cactattcca caaattctcg gctacgaaac
     5761 cccaagtacc tggctttcat ttatgcctca ggtttttgaa tccgttaaag aagaatattt
     5821 cacagtcaaa cttgctgcat taaaaaaaca taaaagccag gaacgacgtg attatatgcg
     5881 tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa caggtcaata gcgatctggg
     5941 tgaaggcttt gtaattcata aaatgattct ttgagcaaat tgcatgaaaa cctggatatt
     6001 tatctgtatg tctatagcaa tgttgctatg gtttttaagt acgctaagac gtaaacccag
     6061 tcaaaagaaa ggctgtattg acgccattat acctgcgtat aacgaaggcc cgtgtctggc
     6121 gcagtcactg gataatctac tgcgtaaccc ttatttttgc cgggtaattt gcgttaacga
     6181 cggctccacg gacaataccg aagcggtcat ggcggaagtc aaacgcaaat ggggcgacca
     6241 ctttgttgcc gtcacgcaaa aaaataccgg taaaggtggt gcgctgatga atggcctcaa
     6301 ttatgccacc tgcgaccagg tttttttaag tgatgccgac acctatgttc cgcccgatca
     6361 agacggaatg ggctatatgc tggcagaaat tgagcgcggt gctgatgccg taggcggcat
     6421 tccctctact gcgttgaaag gcgcggggct gttaccgcac atccgcgcga ccgtaaagtt
     6481 gccgatgatt gttatgaagc gcacgctaca gcagctcctg ggcggcgcac cgtttattat
     6541 cagcggtgcc tgcggaatgt tccgtactga tgtattgcgt aagttcggtt tctcggatcg
     6601 tactaaagtc gaagaccttg atctcacctg gacattagtg gcaaacggct accgtattcg
     6661 gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac agcccgcgtg aggagtggcg
     6721 tcgctggcgg cgttggattg tgggatacgc ggtctgtatg cgcctgcata aaagactttt
     6781 atttagccgc ttcggtatct tcagtatatt tcctatgctg ttggttgtgc tttatggcgt
     6841 tgggatttat ctcactacct ggtttaatga attcatcacc accgggccgc atggtgtggt
     6901 gttggcaatg tttccgctta tctggatcgg cgtagtttgt gttattggtg cttttagcgc
     6961 ctggtttcat cgttgctggt tgttggtgcc tttagcgccg ctttccgttg tgtatgtatt
     7021 attagcttat gccatctgga ttatttatgg acttattgcc ttttttactg gacgcgaacc
     7081 tcagcgcgac aaacccaccc gctattccgc actggtggaa gcgtcaaccg cttattccca
     7141 accttctgtc acaggaactg aaaaactatc tgaagcttaa tgttaaagag aaaaatattc
     7201 agattgcaga ccaggtaatt attgatgaaa ctgcaggtga agttgttatc ggcgcgaata
     7261 cccgtatttg tcatggtgcc gttattcagg gtccggtagt gattggcgca aactgcctga
     7321 taggtaatta tgcgtttatt cgtcctggca caataatcag caatggtgta aaaattggtt
     7381 ttgccaccga aattaaaaat gcggttattg aagcggaagc aacgattggt ccgcaatgtt
     7441 ttattgccga ctcggtagtt gcaaaccagg catatttggg cgcacaagta cgtaccagta
     7501 atcatcgtct ggatgaacag cctgtgtctg ttcgaactcc agagggaatt atcgctaccg
     7561 gatgcgataa attaggttgt tatatcgggc agcgttcacg ccttggcgta caagttataa
     7621 ttttgcctgg gcgaattatt tccccgaaca cgcaacttgg cccgcgcgtg attgtagaac
     7681 gtaatttacc tgccggaact tattcactcc gacaagaact tatccgtaca ggagattaaa
     7741 atgataaaac ggacgttatt agcggcggcc atttttagcg cattgcccgc ttatgccggg
     7801 ttaacttcca ttaccgcggg ctacgatttt accgattatt ctggcgatca tggcaaccgt
     7861 aatttagcgt atgctgaact ggtggcgaaa gttgaaaacg caacgctgct ttttaatctt
     7921 tcccaggggc gtcgtgatta tgaaactgaa catttcaatg ccactcgcgg tcagggtgcg
     7981 gtctggtata aatggaataa ctggctgaca acccgaacgg gtattgcctt tgcggataat
     8041 acgccggtct ttgcccgcca gaattttcgt caggatatta acctggccct gttgccaaaa
     8101 acgcttttca cgaccggtta tcgctacact aaatattacg atgatgtcga agtcgatgcc
     8161 tggcaaggcg gcgtatcact ctatactggc ccggtgatca ccagctaccg ctatacccat
     8221 tatgactcca gcgatgcagg tggtagttat agcaatatga tttccgtgcg tctgaatgac
     8281 ccgcgcggca ctggttatac gcaactatgg ctaagccgcg gaacaggcgc ttacacctat
     8341 gactggacgc cagaaacacg ctacggcagc atgaagagcg tcagtctgca acgtattcaa
     8401 ccgctgactg agcaacttaa tctcgggctg acggcaggta aagtgtggta cgacacccca
     8461 accgatgatt ataacggtct gcaacttgca gcccatctga cctggaaatt ctgattcctt
     8521 ctgccgcccg ctatccgggg cggccttccc tgccgattag cccccccttt cctctttgtt
     8581 ttccgaccac attcaccgga taaattttat tctccagtgt tatatactat aggggggtat
     8641 gcattgacat atagcatacc cccctatagt atattgcgtg cagatgatga ggtgcgaaat
     8701 gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta ttcgagggca
     8761 gattgatgct ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca tactccaaca
     8821 gatcgctgcc gttcggggcg cggctaacgg gctgatggca gaagtgcttg aaagccatat
     8881 ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat ccgttgacga
     8941 cactattgaa ctggttcgag cctatcttaa atagctgaat ctattaccat attgaggaag
     9001 agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg ctggaaatcg
     9061 ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc acccataccg
     9121 gcgtttgcca taccgacgca tttaccctct ccggggatga cccggaaggt gtattcccgg
     9181 tggttctcgg tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc gtaaccagcg
     9241 tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag tgtgagttct
     9301 gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt aaaggcttga
     9361 tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac tacatggggt
     9421 gctctacatt cagtgaatac accgtggtcg cggaagtgtc tctggccaaa attaatccag
     9481 aagcaaacca tgaacacgtc tgtctgctgg gctgtggcgt gaccaccggt attggcgcgg
     9541 tgcacaacac agctaaagtc cagccaggtg attctgttgc cgtgtttggt cttggcgcga
     9601 ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg
     9661 ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg tgctaccgac tgcattaacc
     9721 cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa tggggtatcg
     9781 accatacctt tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg gaaagtgcgc
     9841 accgcggctg gggtcagtcg gtgatcatcg gggtggcagg ttctggtcag gaaatctcca
     9901 cccgtccatt ccagttggtc actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga
     9961 aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat atcgatctgg
    10021 aaccgtttgt cacgcatacc atgagccttg atgaaattaa tgacgccttc gacctgatgc
    10081 atgaaggcaa atccattcga accgtaattc gttactgatt tcccgcaggt ataccccgtc
    10141 cacttcagac ggggttctta atactctccc tgggcagccg tccgggggat taaccgtgag
    10201 ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta
    10261 tcggcattat tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa
    10321 agccgcgaat gaaaaattgc cggtgctgta ctggctttcg ggcctgacct gcaacgagca
    10381 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gaacacaaca ttattgttgt
    10441 tgcgccggac accagtccgc gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg
    10501 gcaaggtgcc gggttttacc tgaacgcaac gcaagcgccg tggaatgaac attacaaaat
    10561 gtatgactat atccgcaacg agctgccgga tttagtgatg caacattttc cggcaacgac
    10621 cagaaagtct atctctggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg
    10681 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
    10741 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagatg cctggttgaa
    10801 ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca
    10861 ggggttgagt gatgattttt acgcggaaca gctgcggact ccaaatcttg aaaagatctg
    10921 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta
    10981 ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg
    11041 ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccgcct acatttcgcc
    11101 cgcaggccgt aggcatgata agacgcgcat agcgtcgcat caggcatctg cgcacgactt
    11161 atctatgttt accacatcaa atcatcaggc actttgaagt cggcatatgg gtcgtcttca
    11221 tcctgctctt cggcgctgag cgcgctgtgc aacactatac tgctggcatc acgctgagct
    11281 attttatcgg caacgctggc gggaataatg gcgtattcac cttcgctatt gttatcgacc
    11341 aacaagcgag caatcgccag acgaccatta atcaactgcg cctgtgtgag cttatcgaca
    11401 aaaatcttct taatcagatt gccgtcagtg aagttaaaac caatatcgcc attggcaatg
    11461 gtgattcggt tcatttcaat gagctgcttc acctgagctt tatattcttt cgccaacgca
    11521 gcttgttttt gttgctcgct aagctgttta tcacgctcaa gctgtgcctt tttattttct
    11581 tctaccgccg cccgagcttc acgcgcctga acgcgcgatt ttttcgccgt tctctccacc
    11641 ttcgccgctt ttttgctggt gactaatcct gctttgagca actgctcttg taaggtaagt
    11701 tttgccatct ttgtttccaa acccgctgaa taatttttag cgagtatacc tgtaaacgcc
    11761 gtgggtgtat tgggggcaac tccctttcgc ccctgcttaa taaggcgcgt caggcatcgg
    11821 cacacggctg cattcttgat ctccgactca tcaaaataaa caccgccaac ccagcaacaa
    11881 gaatacccag tgcagaagcg gccattacgc cgaccgtgcc agtgcctaat gccagcattt
    11941 tcccggccag taacggaccg ctcatagccc ccagacgccc taccgccacg gctgttccca
    12001 cacctgttgc gcggatctgc gaactgtaaa acaacggtgc cagggcatac aaaacgcttt
    12061 gcccacctgt cgcaaacaac cccgcgacaa atcccgccag caacataccg ttaaatgacg
    12121 acaccgttcc aagtgccagc agcgaagcta acatgccgct ataaatcagt agcgacatgg
    12181 tcactggacg cagcttatcc atcaatgcgc ccaacattaa cgtcccgctt gccgcaccca
    12241 tttgcagggc aaacatcacc cctgccgcct gcgatggctg gaatccttgc tccaccaaaa
    12301 gcagcggtag ccagttgatc aacatgtaga ccaccagcag agtgaagaaa taacacaacc
    12361 acagcagcag cgtcgcggtt gccgtttctg gcgcaaataa ggcacgcagt ggtggcgcag
    12421 cctgtttatc gccagcgaaa accgccgact ccggcagcca gcgcattaat agcggcacca
    12481 gaatcaacgg caccacacca cctacccaaa acaccgtttg ccatgctaag tttgcccccg
    12541 cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac accgcaatac atcaggctca
    12601 ctgctgtccc acgaaaacgt ggacccgcgg cttcagacgt cagggcgata agattcggca
    12661 acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa gaccagtgag gggaaatccc
    12721 aggcaatcgc cgttgccagc gagaacaaac caaacagcgc aactgagcca atcaaaatgc
    12781 gcttgcgacc ataacggtcc gccagcattc cgccaaccaa cgcgccgggt agcaaaccga
    12841 gtattccggc gctgaatatc cagcccattt gcattttatc gagtgcgaaa gcctgggcga
    12901 tgccacccgc cgcaatgcca gccgcctgaa gatccagccc ttccatcaga gcgaccaaaa
    12961 aacaaagccc gatagtcagc atcaggcggg atgaagatga tgaaggggta cgagtcgaca
    13021 taagatgaac ctcgttgcga gtgcaggtga atcgcgactg atacattgcc agccgcgtaa
    13081 tcagaactat ttaatgactg cctacgggcg aaatgtaggc cggacaagac gtttatcgca
    13141 tccggcagtc gtgtgcagat gcctgatgcg acgctaacgc gtcttatcag gcctacaaat
    13201 tctcacgcca cccgtaggcc ggataaggtg tttacgccgc atccggcagt cgtgctggga
    13261 tgggcataca aaatgcacgt tctcggcagt catcattatt tgttgttgcg cagatccagc
    13321 gccacgtcaa cgatcatatc ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg
    13381 agaatatcca ccgcacttaa gccataacgc gccgccgccg tttcacagtg acgcaggaag
    13441 ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc gatcgactcg taccggtcga
    13501 tcctgcaacg gacgcaccag gtcgtcagcg gcatccatta acgcatagag atcggtccca
    13561 tgctgccagc ccagtttatc cgcggcggca ataaatactt ccagcggcgc gttacctgcg
    13621 cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc agccctcttc caccgccgcg
    13681 atagagttcg ccacgccaag actcaggtta tggtgagcgt gcatgccagt ttgcgtttct
    13741 ggtttcagct ctgctttcag ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca
    13801 ccgccagaat ccaccacata aatacaggtc gcaccgtagc cttccatcag ctttgcctgc
    13861 tttgcgagat tctccggcgt ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt
    13921 ccgagctcgc gggcatactg aatatgctgg gcggaaacat cagcttcggt acagtgcgtt
    13981 gccacacgaa ccacccgcgc gccagcctgc caggcatttt tcagatcgtg aatagtgccg
    14041 attcctggca gcaacaacgt cgcgattttg gcgtgcttca ccacatccgc cgctgcttca
    14101 atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa agctggaacc ctgcaaaccg
    14161 tcgccatggg ccacttcaat cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg
    14221 cgaacgtttt ccagcgaata ctgatgacga atggcgtgca taccgtcacg caacgtgacg
    14281 tccgagatat aaagtttttt accgttcatg ccgcttctcc tgccttgcgc gccagtgact
    14341 gggccatttt ttccgctgtc gccagcgcac tggaagtcat aatgtcgagg ttgcccgcat
    14401 aggcaggcag ataatgcgct gcgccttcga cttccagcca gaccgctgtt ttcagtccgg
    14461 agaattgccc cacgcccggt aaattgaccg gtttatcctg cgggataact tcaaactgca
    14521 cgcgctgttt caggcgataa cccggtacgt aagcctgcac cgcctcagcc atttcattga
    14581 ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca
    14641 tcatcaacgg tggctctgct gggttaagca caataatcgc cttccctttt gccgcgccgc
    14701 ccaccacttc aatggctcgg gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc
    14761 caggtccggc agatttactg gcgatagaag cgataatttc ggcgtaatga acacgcgcca
    14821 cgcgtgaaac tgccgccacc attggaatgg tggcctggcc gccgcaggtg accatgttga
    14881 cgttcagttg atcgacgttc gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg
    14941 cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt
    15001 ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat gtcgatatca gcaaattcag
    15061 gcatgttcat cagcccgatc accccttcat gggtggtggc gacgcccata cgtctggcac
    15121 gcgccagacc gtcggactga ggatcaatgc cgaccatcac cgccatctcc agatgctgac
    15181 cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt gccagaaccg ataatggcga
    15241 ctttacgctt actcatgaca gacttccttt tggggctgcg cttgaaaatg tcgcagcaac
    15301 tgaacctatg ccttcaatat gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg
    15361 gcctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc ggttcaccca gactggccat
    15421 tttgcgtgcc agccagacgg cggcattaag cggatgtccc aggcattcgc tgccgcgccc
    15481 gctggaaacc tcttcgttat tacgcgtcat cttcatggcg cagtttttca ggtctaaccc
    15541 cgccggacgt tgcgccggac cgccgatgac atacaccccg caggaagcgt tatctgccac
    15601 ggtatcgaca aactgaatcg accagtcgcg aatgcggctc cccaccactt ccagcgccgg
    15661 aagtacccat tcaatggcgt tatacagttc gtcgaaggtg atatcagttg cgggtaaatc
    15721 gcggttcaac accagtgcga tctccgcttc aatgcggggt tgcagaacac gggaaaaagg
    15781 aatgatttcg ttatcgccat aacacatgtc ggcaaataac gtcccaaaat ccggttgatc
    15841 aacgcccagt tgttgttgca ctttcggatg cgtcaggccc actttacgcc ctaccacgcg
    15901 acgcccctgc acaacgtcat attgcacatt tatgtgctga atggcgtagg ccgcttcagc
    15961 gttatcgata ccaatcagat cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg
    16021 gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc atgaccattc cttaagggcg
    16081 tgcgaggaaa ttcagcacca attgattgaa agcgtcggca tgttcccact gcgcccagtg
    16141 accacagtcg cggaagatat gcagttcaga accggcaatg ccggacagca gacgcagacc
    16201 cgcatccatc ggcacaaagc ggtcgttgcg cccccagaca atcagggttt gcgctttgat
    16261 ttccgccaga cgtgggccaa aatccggaaa ctgtttcgga ttagcttcca ggctcttaac
    16321 gaagttttcc aggtgatcgc ggcgcgacag catattattc aggcgcgctt caaacagggc
    16381 gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc atcagcttca ggttttcgat
    16441 agtcggctga cgataaagct gattcagtcg cttaatacct tcggttggca tcggcgtaaa
    16501 caaactcatg ccgcccgtac cgccgcccat cagcaccagt ttgccgacgc gctccggcca
    16561 gttaagggtg aacgccacag aactatggcc gcccatcgag ttgcccagca ggtggatttt
    16621 ggcgatatcc agttgatcca ccacgctttt caggattcgt gcattaagat ccgatcgtga
    16681 accactatta acgatcgaat cgcttttgcc ccaacccgga caatccagca ggattacccg
    16741 atagcccgcc tctaccagcg gatcgatatt gcggctgaag ttcgcccagc cagtagcacc
    16801 cgggccggaa ccatgcagca ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg
    16861 gatgcgcagc gttttacccg cttcttctac attcagaaaa cggctggtgg cggcttcggt
    16921 ttgtggctga taactcatca tcttctcctg catattcagt tctctgttct ggcgcttaac
    16981 gagccaaatc cggcaatcca ctccgggatt gggcggtaat aacgcccttc gctacgccag
    17041 ttgccaaatg tagaaatagc ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac
    17101 tttccggcaa tggcggaaag ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc
    17161 tgctccagca aagtcatgaa ctggttatcc caaatcgggt tgagcggatg cagagttctc
    17221 tgatcctcaa caaacttctc agcggcgcta atcacccgtt gctgacgcaa ttcgcgctca
    17281 ctggcgggta aatctttccc gctccccaac agacggtcgc gcatatgggc atcggctttc
    17341 gccagttcgg gcaccggcgg ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt
    17401 ttattgagag tgctggtgaa acgtccaatg gcttcaccca acatgcgggt acgctggaaa
    17461 ccgggcagcg gcgtggcgac accgttgatg aacacaggca gaactggcac cttatccagc
    17521 ccaccgagca ggaactccag cggctgggcg aacccgtggt ccacctgcat acagtaagaa
    17581 acggcaagat cgatcccgct cttcatgaca gcatgcgcac aggcctccgc cagctccaca
    17641 ggcacgggca gctctcctgc cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct
    17701 aaacagaacg gtggcatcac gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc
    17761 accaccagtt caggggagaa agccgcaata cgctcgcggg cgctggcaat cacgccattg
    17821 acctcatcga gtacctcttg cgccgggtcg acatacccca ccaacggcga gtgggaaaga
    17881 cagtgaagat aagcgtgcat atcaggctac cttttcgaca gaaacgtcgg catcagggcg
    17941 ggtcagcgtc atcaccgacg ccagtttatt cagcgtattg cccagggttt gcggaatggc
    18001 ggtggcggca acaaagcgat ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa
    18061 ccagctacgc agacgacctt gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg
    18121 tgcggtatga atttgcactt ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca
    18181 ctgctggatt tgctcgtcgc tcatccccca cagtggattg catccccagc caattaccgc
    18241 gaagttcgcg ccaatcgcgt tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt
    18301 cggctgaata aacatcttgc cgaccggaga gtgcttcgct tcgccctcac gtaccagcgc
    18361 accgccgtaa tattgcggca tcggcttgaa gcgcatttcg aggaagtagc gttttactgg
    18421 cggcagataa ttcagtagcc aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg
    18481 cggagccagc acgttgcccg ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg
    18541 acgttcttgt tgataggtat cgagcagcgc atcgcgggct ttcccctgga taaccaacgc
    18601 cagtttccag gcgaggttaa aggcgtcgcg cataccactg ttatagccct gcccctgcca
    18661 caccggcatg atgtgcgcgg catcgcccgc cagcagtacg cgatcaatac ggaaacgttg
    18721 cgccagtcgc gcgttatggg tgtagacacg ctggcgaatc aattcaacat tgtccggatt
    18781 aggcagcact ttgcttaaca gcttgcgcat attttgcggc tcacgcagct gctcttcggt
    18841 ttctcccggc atcaccataa attcaaagcg acgtaccgca tgaggcagcg cggcagaaac
    18901 atacgggcgc accggatcgc aacacaaata gatatgcggc gtacttaacg gatcgttggc
    18961 gatatctacc acaatccact gatttggcgc agttttacct tcaaacggca cattcagagt
    19021 gcgacggaca aaacttgctc caccgtcaca ggctaccagc cactgggctt tgactatttc
    19081 ccgctgccct tctgccgttt tcaggtgcaa ggtcacttcg tcattttgct ggctgaaggc
    19141 ctccagctcg cgggaaaaca agcagcgcac attaggaaaa cgcgacaccc cttccagcat
    19201 caccgcatcg acctgcggct gaataaaggc gttacggcgc ggccagccaa attcatcggt
    19261 cattggctga atatcagcaa aacagcggcc tttcggggtg agaaaacgca tcgcgtgcca
    19321 cggcgtagtg tgcggcagaa cattctcgac caggccaacc gactgcatgg tgcgcagcgc
    19381 ctcgtcatca ataccaatcg cacgcgggta gtcgatcaac ttatcgagtt tctccaccac
    19441 cagcacgtca atgcccatct gaccgagata gttcgccatc atcagcccga ccggaccggc
    19501 acctgcgatc gccacctgaa cgctatggtt aacagcaggc tggatgtcag ggtgttgtat
    19561 tgccatttca gtacctcacg actcggacaa aatgccgttg cgcgcacagt acggcgcaac
    19621 ttattttgtt aaaaacatgt aaatgatttt ttgttgtgcg ctcagtatag gaagggtgtt
    19681 ttcggctaca atcaaaacat gcccgaatgt gcaccaggtg cacaacgttg ttttaactat
    19741 agaaatgtca attaatatgc agaacaatga gcagacggaa tacaaaaccg tgcgcggctt
    19801 aacccgcggt ctaatgttat taaatatgtt aaataaactt gatggcggtg ccagcgtcgg
    19861 gctgctggcg gaactcagcg gcctgcatcg caccactgtg cggcgactgc tggagacgct
    19921 gcaggaagag ggatatgtcc gccgtagccc ctccgatgat agttttcgac tgaccatcaa
    19981 agtgcggcaa ttaagcgaag gatttcgtga cgaacagtgg atttctgcac tggcggcccc
    20041 actgctgggc gatctgttgc gcgaagtggt atggccgaca gatgtgtcca cgctggatgt
    20101 tgatgcaatg gtggtacgcg aaaccactca ccgtttcagc cgcttatcct ttcaccgggc
    20161 aatggtcggg cgacgtttgc cgcttctgaa aaccgcctcg ggcctgacct ggctggcctt
    20221 ttgcccggaa caagaccgca aggaattaat cgaaatgtta gcctcccgcc ccggtgatga
    20281 ctatcaactg gcacgggaac cgttaaagct ggaagccatt ctggcgcgcg cgcgcaaaga
    20341 gggttacgga cagaactacc gcggctggga tcaggaggag aagatcgcct ctatcgccgt
    20401 accgctgcgc agtgaacaac gggtgattgg ctgtctgaat ctggtgtata tggcgagcgc
    20461 aatgaccatt gaacaggcag cggaaaagca tcttccggcg ctacaacggg tagcaaaaca
    20521 gatcgaagaa ggggttgaat cgcaggctat tctggtggcc ggacggcgaa gcggcgtgca
    20581 tttacgttga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgttcgg
    20641 aagagagtca attcagggtg gtgaatgtga aaccagtaac gctatacgat gtcgcagagt
    20701 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
    20761 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
    20821 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
    20881 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgctgat caactgggtg
    20941 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
    21001 acaatcttct cgcgcaacgc gtcagtgggc tgctcattaa ctatccgctg gatgaccagg
    21061 atgccactgc tgtggaagca gcctgcgcta atgttccggc gttatttctt gatgtctctg
    21121 accagactcc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
    21181 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
    21241 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
    21301 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
    21361 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
    21421 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
    21481 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
    21541 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
    21601 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
    21661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    21721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    21781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tgaaattgtg
    21841 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt
    21901 cgttttacaa cgtcgtgact gggaaaaccc tggcgttaca caacttaatc gccttgcggc
    21961 acatccccct ttcgccagct ggcgtaatag cgaagaagcc cgcaccgatc gcccttccca
    22021 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
    22081 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtca tcccctcaaa
    22141 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
    22201 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
    22261 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
    22321 gtttcatctg tggtgcaacg gtcgctgggt cggttacggt caggacagtc gtttgccgtc
    22381 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
    22441 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
    22501 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
    22561 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
    22621 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
    22681 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtagttatg ccgatcgcgt
    22741 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
    22801 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
    22861 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
    22921 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
    22981 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
    23041 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
    23101 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
    23161 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
    23221 tcacccgagt gtgatcatct ggtcgctggg gaatgagtca ggccacggcg ctaatcacga
    23281 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
    23341 cggagccgac accttcgcaa ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
    23401 ccaacccttc ccggcggtgc cgaaatggtc catcaaaaaa tggctttcgc tgcctggaga
    23461 actgcgccca ctgatccttt gcgaatatgc ccacgcaatg ggtaacagtc ttggcggttt
    23521 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
    23581 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt tggcttacgg
    23641 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgc atgaacggtc tggtctttgc
    23701 cgaccgcacg ccgcatccgg cgctgacgga agcaaaatac cagcagcagt ttttccagtt
    23761 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
    23821 cgagctcctg cactggatgg tggcgctgga tggcaagccg ctggcaagcg gtgaagtgcc
    23881 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctggactac cgcagccgga
    23941 gagcgccgga caactctggc ttacggtaca cgtagtgcaa ccgaacgcga ccgcatggtc
    24001 agaagccgga cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagcgtgac
    24061 actccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
    24121 cgagctgggt aataagcgtt ggcaatttaa ccgtcagtca ggccttcttt cacagatgtg
    24181 gattggcgat gaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
    24241 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
    24301 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgct cggcagatac
    24361 acttgccgac gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg gaaaaacctt
    24421 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
    24481 tgttgaagta gcgagcgata caccgcatcc ggcacggatt ggcctgacct gccagctggc
    24541 gcaggtcgca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
    24601 ccttactgcg gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
    24661 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
    24721 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
    24781 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
    24841 ccatatgggg attggtggcg acgactcctg gagcccgtca gtgtcggcgg aattccagct
    24901 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
    24961 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa aaaacacaaa
    25021 cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag cctacttccc
    25081 gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata cgggtattat
    25141 ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc tgctttctga
    25201 caaactcggg ctgcgcaaat acctgctgtg gattattacc ggcatgttag tgatgtttgc
    25261 gccgttcttt atttttatct tcgggccact gttacaatac aacattttag taggatcgat
    25321 tgttggtggt atttatctag gcttttgttt taacgccggt gcgccagcag tagaggcatt
    25381 tattgagaaa gtcagccgtc gcagtaattt cgaatttggt cgcgcgcgga tgtttggctg
    25441 tgttggctgg gcgttgtgtg cctcgattgt cggcatcatg ttcaccatca ataatcagtt
    25501 tgttttctgg ctgggttctg gctgtgcact catcctcgcc gttttactct ttttcgccaa
    25561 aacggatgcg ccctcttccg ccacggttgc caatgcggta ggtgccaacc attcggcatt
    25621 tagccttaag ctggcactgg aactgttcag acagccaaaa ctgtggtttt tgtcactgta
    25681 tgttattggc gtttcctgca cctacgatgt ttttgaccaa cagtttgcta atttctttac
    25741 ttcgttcttt gctaccggtg aacagggtac gcgggtattt ggctacgtaa cgacaatggg
    25801 cgaattactt aacgcctcga ttatgttctt tgcgccactg atcattaatc gcatcggtgg
    25861 gaaaaacgcc ctgctgctgg ctggcactat tatgtctgta cgtattattg gctcatcgtt
    25921 cgccacctca gcgctggaag tggttattct gaaaacgctg catatgtttg aagtaccgtt
    25981 cctgctggtg ggctgcttta aatatattac cagccagttt gaagtgcgtt tttcagcgac
    26041 gatttatctg gtctgtttct gcttctttaa gcaactggcg atgattttta tgtctgtact
    26101 ggcgggcaat atgtatgaaa gcatcggttt ccagggcgct tatctggtgc tgggtctggt
    26161 ggcgctgggc ttcaccttaa tttccgtgtt cacgcttagc ggccccggcc cgctttccct
    26221 gctgcgtcgt caggtgaatg aagtcgctta agcaattaat gtcggatgcg ccactgggca
    26281 catccgacaa ttatttatag ctccatcatc tggcgaaaca agcgtcgcat catacctctt
    26341 tgacccacag ctgcggaaaa cgtgctggtg caaaacgcag ggttatgatc atcagcccaa
    26401 cgacgcacag cgcatgaaac gcccagtcca tcaggtaatt gccgctgata ctacgcagca
    26461 cgccagaaaa ccacggggca agcccggcga tgataaaacc gatcccctgc ataaacgcca
    26521 ccagcttgcc agcaatagcc ggttgcacag agtgatcgag cgccagcagc aaacagagcg
    26581 gaaacgcgcc gcccagacct aacccacaca ccatcgccca cgataccggc aattgcagcg
    26641 gcaaccagat aaagccgcag aaccccacca gttgtaacac cagcgccagc attaacagtt
    26701 tgcgccgatc ctgatggcga gccatagcag gcatcagcaa agctcctgcg gcttgcccaa
    26761 gcgtcatcaa tgccagtaag gaaccgctgt actgcgcgct ggcaccaatc tcaatataga
    26821 aagcgggtaa ccaggcaatc aggctggcgt aaccgccgtt aatcagaccg aagtaaacac
    26881 ccagcgtcca cgcgcgggga gtgaatacca cgcgaaccgg agtggttgtt gtcttgtggg
    26941 aagaggcgac ctcgcgggcg ctttgccacc accaggcaaa gagcgcaaca acggcaggca
    27001 gcgcccacca agcgagtgtt tgataccagg tttcgctatg ttgaactaac cagggcgtta
    27061 tggcggcacc aagtccaccg ccgcccatca gagccgcaga ccacagcccc atcaccagtg
    27121 gcgtgcgttg ctgaaaccgc cgtttaatca ctgaaggcat caccgcctga atgataccga
    27181 tccccacccc accaagcagt gcgctgctaa gcagcagcac actttggggg taaagctcac
    27241 gcatcaatgc accgacggca atcagcaaca gactgatggc gacactgcga cgttcgctga
    27301 catgctgatg aagccagctt ccggccagcg ccagcccgcc catggtgacc accggaagag
    27361 cggtcaacag ggcagccacg ctaaagctca ttccgctcgc ctggcgcaat tgcggtagca
    27421 gtggcccgac ggaggtgagc agtggtcgca tattaagacc aatcagcacc agaaccagca
    27481 gcatcgtgaa cgtcttttga tgattgcgca tggctgtcag aacggcttgg tcggcagata
    27541 tttaccatct aaggtaatga ccgcacgttc gccaccttcc gggtccgcca ctttcttaac
    27601 gtcgagtttg aagttaatcg cgctgataat gccatcgcca aacttctcat gaaccaacgc
    27661 tttcagggtt gtgccgtaca cctgcaacat ttcatagaaa cgatacatcg ttgggtcggt
    27721 tggaatacgg tcatcaatgc agccacgcag tggaatcatc tgcaacagta gaatggcgtc
    27781 ttcgtcgaga tccagcttcg ccccgaccag gcgggcggcg tcggcaggaa gcgcttgctg
    27841 ccccagcaaa gccgcggtta cgaaggcttc tgccagaccg gtaccgtcgg caatctcggc
    27901 aaatgagaga tcttttttag ctttgctgag caaaatggca tcggcaagat caagacgaat
    27961 attgcggtta atttgtgact gaatcatggt ggatctcctg atggtttaaa aataaggtta
    28021 cgctgcggtc ggttggcgta gcggtatggc acaaacgcga ggattagcgg ccagtggcac
    28081 aaactggcgg gttgcgccgt caaaagctgc gatgctgccg ctttcaatgt cgtagaccca
    28141 gccgtgcagg gcgatccgcc cctcttcgag cgccaggcgc accgatggat gagtttgcaa
    28201 attagccaac tgagcaatga cgttttcacg taccatcgcc gcagcttttg acggtaaatc
    28261 ggaatgcggg cgcgcctcat taacgacgcg ggctgaatcg gcataacgca gccagtggga
    28321 gacggcaggc atatggtcca tgcactgaca gctggcaatg gcggtcatcg cgccacagtt
    28381 ggaatgacca caaatcacaa tgtcagatac ccgaagcgca gcgacggcat actccaccga
    28441 agcagaaacg ccaccgggtt ccggcccgta ggaagggacg atattgcccg cgttgcgaat
    28501 aacgaacaga tcgccaggct cacgttgcgt caccagctca gggaccagac ggctgtcgga
    28561 gcaggagata aaaagtgtgc gcgggctttg ctgtgtcgcc agctgtttaa acaaggcttc
    28621 ccgcttcgga aatgcctcgc gctggaattt aaggaatcca tcaataatct ctttcaccgt
    28681 taacctctgt ctgtctctgg aatgagaggc agactactcg ccgattatca taaggtaaaa
    28741 gtctcattta tgatgagttc tattggattt acttataggt tggcgaatgc tctctcgaca
    28801 tatcaattat tttcttgccg tggctgaaca tggcagcttc acccgtgccg ccagtgcgtt
    28861 gcacgtctct cagcctgcgc tttcccagca gattcgccag ttagaggaga gtttaggcgt
    28921 gccgctgttt gaccgtagcg ggcgaacgat tcgtctcacc gatgcaggag aagtctggcg
    28981 acagtacgcc agccgggcgt tacaggaact gggagcgggt aaacgggcga ttcatgatgt
    29041 tgccgatctg acgcgaggat cgctgcgtat cgccgtcacc cccaccttta caagctactt
    29101 tatcggccct ttaatggcgg atttctatgc gcgctacccc ggcatcacgc tccagctaca
    29161 ggaaatgtcg caggagaaaa tcgaagatct gctttgtcgc gacgagctgg acgttgggat
    29221 tgccttcgcg cctgtgcatt cgccggagct ggaggcaatt cctttactga cagaaagttt
    29281 agcgttagtc gtggcgcaac atcatccgct ggctgcctgt gaacaggtgg cgttgagtcg
    29341 cttgcatgat gaaaaactgg tcctgctcag cgcggaattt gccaccagag agcaaattga
    29401 ccactactgc gagaaagcgg ggctacatcc acaggtggtc attgaggcga actcaattag
    29461 cgcggttctg gagctgattc gccgcacttc cctttccaca ttgttaccag cagcgattgc
    29521 cacacaacat gacgggctta aagcgatttc tcttgccccg ccactactgg agagaacggc
    29581 ggttttgttg cggcggaaaa atagctggca gacagccgcc gcgaaggcat ttttgcacat
    29641 ggcgttggag gaatgtgcgg atgttggcga gaatgaatca cggtagccac tgacacaggt
    29701 cagtggctct tcaacacaga ggaaattacc gtttgtaatc gatggcttct ggctgctcca
    29761 gatatacggt ggtttgtgcc ggttgtgtac tggcaatcac cttgccgcca cgtaccgaat
    29821 aacgtaccgg aacctgacgg cgcagcgcat caaatccatt ttcagccggc aggataatca
    29881 ggtttgcgct gtttccggcg gcaatgccgt aatcctgcaa attcaatgtt ctggcactgt
    29941 ggtgggtgat taaattcagg ccatcgttaa tctgcccgta gcccatcaac tggcaaacat
    30001 gcagccccat atgcagcact tgcagcatat tcgccgttcc cagcggatac cacggatcga
    30061 agacatcatc gtgaccaaag cagacgttaa tgccggactc cagcatctct ttaacgcgcg
    30121 tgatgccgcg acgttttgga tacgtatcga aacgtccttg cagatgaata ttgaccagcg
    30181 ggttggcgac aaagttaata ccggacattt tcagcaagcg gaacaggcgt gaggtatacg
    30241 ccccgttata ggagtgcatt gccgtggtgt ggctggcggt gactcgcgcg cccatgcctt
    30301 cacggtgcgc cagggcagca acggtttcga caaagcgcga ctgctcgtca tcgatctcat
    30361 cacagtgaac gtcgatgaga cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg
    30421 actccacgcc gtattcacgg gtaaattcaa aatgcggaat cgcccccact acatctgccc
    30481 ctaagcgtaa cgcctcttcc agcaacgctt caccgttggg atacgacaaa atcccttcct
    30541 gagggaaggc gacaatttgc agatcaatcc acggcgcgac ttcctgcttc acttccagca
    30601 ttgctttcag cgcagttagc gttgcatccg aaacatcgac atgggtacgc acatgctgaa
    30661 tgccgttggc aatctgccat ttcagcgttt gccaggcgcg ttgtttcaca tcgtcatggg
    30721 ttaataacgc tttgcgctcg gcccagcgtt caatgccttc aaacagcgtg ccggactgat
    30781 tccagttcgg ttgtccggcg gtttgcgtgg tgtccaggtg aatatgcggc tccacaaacg
    30841 gcggtataac taaaccttgt tcggcatcca ggctgttttc agttatgggc atcacgccgg
    30901 attgcgcatc aatggcgctg atttttccgt cctgcagatg aatctgccac agcccctctt
    30961 tgcctggtaa ccgggcgtta ataattgttt gtaaagcgtt attcgacact gttaacctcc
    31021 acatgcgtca ttgctgctgt cgttttacga ttcaaaatcg ggttaaggat cagatagctc
    31081 agcgcgccac ctaataccgc gttgaccgga acaattcccg gtaaccagtg gcctgcggca
    31141 atccccagcg cgaccgccag aatcgccacc caattgacac tcatcatacg cgtggtcgca
    31201 aagtgctcat agcggcgacg gttcatcaga tagtcggcga tgatcacgcc ccctactgga
    31261 ggaatagctg ccgaaaggaa ggtcaaccag ccgacaaaat tgttatacag ccataatgcg
    31321 cagaccgtac cgataatacc gttgattacc gaaagggttt tgctcgacat accggtaatg
    31381 ttggcgaaac ctaaacccga cgcatagagt gcgttatcgt tggtggtcca gatattcagc
    31441 cccagcacca caatcgcagg cagcagcagg ccctgagcaa tcatcacatc agagatatcc
    31501 gccatgccca gtgccgcagc gcccgctgca ccgaaaataa acatcaacga gttgccgagg
    31561 aaaaaggcca ccatcgccac cagcaccgcc agtttggcat tgcgaccaaa ccggacaaag
    31621 tcagcggtga gcgtacccgc actgataaat gaccccacaa ccagcgccag cgcgacattg
    31681 aaatctaacg gttgtgcggg aacgaccgct tttaatgcgt ccaggccgcc catgccgtta
    31741 acagccagcc acacggaata accgcccagg caggcgatag ccggaaccgc aatcaccgaa
    31801 agaaccgtca gcgccgaaat gccaaaaaag acggtgacgg tcatcagtaa accggaaacg
    31861 gcaatcagca aattaatatc cagcccggtt gccttaccca ccggaatggc aaacatcgcc
    31921 acaccgacgc caaaccagcc aacctgagtt ccgcccagta gcagtgaagg cagccatgag
    31981 cctttaacac caaacgagaa gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca
    32041 atgtaaccga gaaatgaagt gtaaataccg aggagaagat taccgatgag aactgcgagg
    32101 aagaaatcat gatagctaag accggttccg agagtgccgc cggtccacat actggcggaa
    32161 aagaaggtta atcccagcat gacgaacgtc aatgccaata cccctttccg cgccgactgc
    32221 gggactggcc cctggctaaa gttgttatct tgcgacacga aattccccct ttttgctgtt
    32281 tgaaacccaa aaaatccgcc gcattctatt catctgaaga taaaaagcaa tcgttttcgt
    32341 ggggaaatat attttttata tgaatgagtg caaaaaggtg ggggaaaaat tatcggtgta
    32401 aaaacagtgg gtagtgggta gatactgata agacgcgtca gcgtcgcatc aggcatcggc
    32461 gcggggtgtc ggatgcgacg cgagcgcctt atccgaccta cggttcaggc ctgtaggcat
    32521 aataagacgc ggtaagcgtc gcatcaggca tcggcgcatg gtgtcggatg cgccacgcga
    32581 cgcatccgac aataccctac tcttccatcg cctggcggat ctgatccaat gacgcaggat
    32641 catcaatggt cgtcagatcc ccaggatcgc gtccttcgca aattgcctgg atcgtgcggc
    32701 gcagcatttt tccggatcgc gttttcggca attgcgagac aaaccagacg tgcgccgggc
    32761 gaccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct tgcgaatgcg
    32821 ccacatcacg atcttccaga ctatcgctct ctttcggaat gacaaacgcc accgccacct
    32881 gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc ggatggctgg
    32941 agatactctc ttcaatctcg cgcgtgccca gacgatgccc ggcaacgtta attacatcgt
    33001 cagtgcgccc gagaataaag tgataaccgt cagcgtcacg gatgccccag tcaaaggtgg
    33061 cgtacaccgg gcgggaaaac agcgaccagt aagtcttcac aaagcggtcg tcgtcgcccc
    33121 agatggtctg aatacagccc ggcggcagcg gcccttccac caccagcatc cctttctcgt
    33181 tgacgccaca cggttcgccg gtgacttcat taagcaactg cacgttatag ccatacatcg
    33241 gcacgccggg gcttcccaga cgcgtcggcc tgtcgtccag accgcgagca atcgccataa
    33301 tcggccagcc ggattcggtc tgccagtagt tgtcgatgac cggcacatcc agcgtattgc
    33361 tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc acttccagcg
    33421 acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg cgaatggcgg
    33481 tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac cacacgccgc
    33541 agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc catccccgcc agcagcggcg
    33601 cgtaaacgat atacgaatgc cccaccaccc agccgatatc cgatgcgcaa aagaacacgc
    33661 tgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc cagcgccacc gcatatccgc
    33721 cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag agaatgcagg
    33781 aggtttcgtt ggattccagc cacgccaccg gtacccgcgc gccgatgtgt tgatggcgca
    33841 acgacgcgaa atcgacatcc cgcccgctga cgcgcgccat tttcgccagc ccgcgatcca
    33901 ccagcaaaac atggcgtggc tggtgctgcg cctgacttat cgcatcgtcg agcaattttt
    33961 tatagggaat gattttgcca ccgcgcgctc cggcatcagc cgagacaatc agcaccggtt
    34021 tagcgtcatc aattcgcgcc gccacgctgt gcgaggcaaa accaccaaat accaccgaat
    34081 gaatagcacc aatgcgcgcg caggccagca gagtaatatg cgcttcggca atcatcggca
    34141 tatacaccag cacccgatcg ccgcgctgca cgcccagtga gcgcaacatt gaggccaccg
    34201 cgttcacttc gtcatgcagc tggcgaaagg taaaggtgcg ctcttcttct gtttccgaag
    34261 agacggcaat caatgccagt gcctctggct gtttctccag ccagcggtcg atagcgttgt
    34321 ggcacaaatt ggttcggcct tcacaaaacc aacgggcaaa cggcggattg ctgtggtcga
    34381 gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc cagaactgct
    34441 ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc tcctgttgaa
    34501 cttacgcctt accgccgttt acttaaatga cgtacaggtc gagatactca ttgaccggca
    34561 tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgt tggcgagtcg
    34621 ggaactggcg cgcgagattg attttgaatt tatcgaccag tttcggaata ccatcctgac
    34681 ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaatcgt gtgccgtcgg
    34741 tgaactcaag ggtaatggca ttggcgatgg cgcgtttttc cgggtcgtgg tagtcagcgg
    34801 taaatgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg cgtttatctt
    34861 gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc gaataacagc gggatcgcca
    34921 ccatgtactg aatgcagtgg tcacggtcag ccgggttatt gagcggcccc tttttgtcga
    34981 tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc gccgccgtct
    35041 tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgcc gtctgggagt
    35101 ggaactccgc cgggaaggag attttgaaca gcacattctc catgacgtaa gaaccgtacg
    35161 gacgctggaa gcggaacgat tcacctttaa aggagacgtc gtagaagccc cataccggcg
    35221 cggtcagggc tgacgggtaa cccatttcgc ccgttttcgc catcagtgcc agacgtaccg
    35281 cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtgttc ggcgcatggc
    35341 gataggtgcg cagcgactga ccgtccaccc acgccagcga aacggcgttg agaatttcct
    35401 cgcgggtcag gccgagcatt tcggcgacca cggcggtgga agccactttc actaacagaa
    35461 cgtggtcgag gccgacgcgg ttaaaggagt tttccagcgc gatgcagccc tgaatttcat
    35521 gggctttgat cattgcggtc agcacctgtt tcatggtcaa cggcgctttg ccgctggcga
    35581 cagcgttgcg cgaaagccag tctgccgttg ccagaatgcc gccgaggttg tcggaaggat
    35641 gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccagcggatc atcgcgccaa
    35701 tgttaaatgc cgcctggaca gggtcgagct gaaactgagt tccggggacg cgcacgccgt
    35761 tgggtacgac ggtgccagga acaattggcc ccagcagttt tttacaggcc gggtattcga
    35821 gagcttccag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg tcgtaggcca
    35881 ctttagagct gatttcgtag ttcatcacgt aatcgacgat atcaacgatt tcacgatcaa
    35941 attccgggcg gatgttgttg atttgagctg acattgggta tgtttcctta ttgttattcg
    36001 tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt ccaacataat
    36061 tggcggaagg acggataatt ttgttgtcct gacgttgttc aatgatgtgc gccgcccagc
    36121 cggtgacacg ggcgatgaca aacagtggtg tgaacatctc ggtgggaacg cccatcatgt
    36181 tgtaggaaac agcagagaac cagtcgagat tggggaacat ctttttgctc tcccacatca
    36241 ccgtttccag gcgattggcg atgttgtaca tcttcaacga gccgccttcc tgcgagagct
    36301 gcttcgccac acgtttgatc acctggtggc gcgggtcggc gatggtataa accggatgac
    36361 caaaaccaat gaccacttct ttgttttcca cccgcttgcg gatatcggct tcggcttcgt
    36421 ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgctccg ccgtgtttcg
    36481 gcccgcgcag tgcgccaatc gcgccaataa tcgcggaata catatcagag ccagtgcccg
    36541 caatcacccg actggtaaag gtggaggcgt taaactcgtg ttcggcgtac agcactagcg
    36601 aaatatgcat cgccttttcc cagctttgcg atggcttttc gccgtgcagc agatgcagga
    36661 agtgaccacc gatggagtcg tcatcggttt ccggctggat gcgttcgccg ttgtgactgt
    36721 agtgatacca ataaaggaga atcgagctaa gtgatgccag cagtttgtcg gcaatatccc
    36781 gcgcgccaga gacagtgtgc ccctcttttt ctggcagtgt gcagccgagc gcggaaacgc
    36841 cggtgcgcat gacatccatc gggtgcgacg ccgccggtaa ggcttccagc acggtacgca
    36901 cgttagccgg taaaccgcgc agggctttca gtttcgtttt gtaagcggcg agttcgtcac
    36961 gggtcggcag tttgccgtgg atcagcaggt gcgccacttc ttcaaattcg caatgttccg
    37021 ccagatcaag aatatcgtag ccacggtaat gcaggtcatt gccactttta cccacggtgc
    37081 agagcgccgt attgcccgcc ggaacgccag aaagtgccac agattttttc ggtttaatga
    37141 catgggtact gttttgcagg atcgttgtgt cgctcatgtt gtcctcgtca tttgtagggt
    37201 cgagatattt ttcgttgtgt tttatttcac ctggctacgg gcaaacaggt cgtcgagctt
    37261 cgcttcgtac tggtagtagt tgatgctttc gtacagctcg ttgcgggttt gcatggtatc
    37321 gatgacgctt ttctgcgtgc cttcctgacg caggacgttg tagacatgct cagcggcgcg
    37381 gttcatggcg cggaacgctg aaagcgggta cagcgccatt gcgacatggg cgctgcgtaa
    37441 ttcgtcgatg gtaaacagcg gcgtggcacc aaattcggta atgttggcga ggattggcac
    37501 ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg gtaatcgcct ccgggaacaa
    37561 catctcggca cccgcttcaa cataggcctg cgcacgctcg atcgccgcat ccagcccctc
    37621 taccgccaga gcatcggtgc gcgccatgat cacaaaatca ggatcggttt tcgcatccac
    37681 cgccgcgcgg atccgatcca ccatctcttc tttcgagacg atcgctttat tcggacgatg
    37741 accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat cctgccgcac cggctttaat
    37801 catcgatttc acggtgcgcg ccacgttaaa ggccgaagaa ccaaaaccga tatccgcatc
    37861 caccagcagc ggcagcgaac aaacgtcggt gatacggcga atatcggtca gcacgtcatc
    37921 aagagtagaa ataccgagat cgggcagccc cagcgaacct gccgccacgc caccgccaga
    37981 aagataaatt gcctgatatc cggcacgctg cgccaacagc gcatgattag cgttgatggt
    38041 gccaacaatc tgcaatggat tttctttagt cagtgcagcg cgaaacgctt tacctggaga
    38101 gtgtagagac atagcccatc ctttgttatc aacttgttat ttgcgttgat aaagacaaag
    38161 caaggggtgt gccacaattg ccttaataaa attaaatgtt taatttcaat ttgttagaga
    38221 taaagtcatg aactaatccg caaatatgcg tttcagttaa cgtttcaggc aatgtttcac
    38281 cgcgtttcat tgcaacaatt atgaaacaag actaaaccca atattcggtt tcttaacttt
    38341 gcggtgcgct atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt
    38401 atctgtaacg cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc
    38461 gaacattacc cctatccagc ttggctttga aaaagcggtg acctacatcc acaagaaact
    38521 ggcaaacgaa cgctgtgacg ccatcatcgc ggctggatct aacggcgcgt acctgaaaag
    38581 ccgcctgtca gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact
    38641 ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc acttatcagg aaactattcc
    38701 ggcactggtg gcgtttcaaa aaacctttaa tttgcgcctc gatcaacgta gctacattac
    38761 cgaagaagac gcacgcgggc agattaacga gctaaaagct aacggcaccg aagcggtggt
    38821 cggcgcgggg ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta
    38881 ttccgccgcc accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt
    38941 acgccataac actcacgatg ccactcgcaa cgccctgcgt actcgttacg tgctgggcga
    39001 tatgctcggt caatcaccac agatggaaca ggtgcggcag actattttgc tgtatgcccg
    39061 ctccagtgcg gcggtgttga ttgaggggga aacggggacg ggcaaagaac tggcagccca
    39121 ggcaattcat cgggaatact ttgcccgcca cgatgcgcga cagggcaaaa agtcgcatcc
    39181 gtttgttgcc gtcaactgcg gggcgattgc cgaatcgctg ctggaagcgg agctgtttgg
    39241 ctatgaggaa ggggcgttta ccggctcgcg acgcggaggt cgcgccgggc tgtttgaaat
    39301 cgcccacggt ggcacgctgt ttttggatga gattggcgaa atgccgctac ctttgcagac
    39361 ccggctgctg cgggtgctgg aagaaaaaga ggttacccgc gtcggcgggc atcagcctgt
    39421 tccggtggat gtgcgggtca ttagcgccac tcactgcaag ctggaagaag atatgcggca
    39481 agggcagttt cgccgtgacc tgttttatcg gctgagcatt ttacgcctgc aactgccacc
    39541 actgcgtgag cgggtggcgg atattctgcc gctggcggaa agctttttga aagtgtctct
    39601 ggcggcgctc tccgccccgt tttctgccgc attacgcctg ggattacagg caagcgaaac
    39661 cgtgctggtg cactacgact ggccgggcaa tattcgtgaa ctgcgcaata tgatggagcg
    39721 actggcgcta tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct
    39781 gctactgccg gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc
    39841 acaacaggca ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat
    39901 cagccggacg acgttctggc ggcggctgaa aagctgaact gccgaatgca gcgcgaacgc
    39961 tttatccggc ataaatttca atagatgatc accgataaac ttacgcatcg ggcatgttta
    40021 catagggttc agaattactt cttcttataa atatttgccg tgccgtggat cttattgtca
    40081 gtttgaccgg aggtcagtac caacacatca gccccttttt catccgcttt tttgatcaaa
    40141 tcttctttcg cgtctgcagt cgacatttca ttgctggttg aaatatcacc tattttttca
    40201 tactgcgatt caactttttc aaattccgct ttggtcatca attcagcggc atagacattg
    40261 gtaacggcta acgctaacgc accgactaac attttagaga ttattttcat attacggctc
    40321 cagagttgtt atcacactat catccaccca cattattggg ttgattaata attagttcag
    40381 tcagggcata aacgccagta accggaaata agacactttg tgatctctct cgcacccgat
    40441 tatttttggt agaaatatga tgaagttact tcacttattt atggatgaaa tcactatgga
    40501 tcctttgcac gccgtttacc tgaccgtagg actgttcgtg attacttttt ttaatccggg
    40561 agccaatctc tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg caggggtgct
    40621 gaccgggctg ggcgtggcgc tgggcgatgc attttattcc gggttgggtt tgtttggtct
    40681 tgcaacgcta attacgcagt gtgaggagat tttttcgctt atcagaatcg tcggcggcgc
    40741 ttatctctta tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac
    40801 actacaacaa ccgattagcg ccccctggta tgtctttttt cgccgcggat taattaccga
    40861 tctctctaac ccgcaaaccg ttttattttt tatcagtatt ttctcagtaa cattaaatgc
    40921 cgaaacacca acatgggcac gtttaatggc ctgggcgggg attgtgctcg catcaattat
    40981 ctggcgagtt tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg
    41041 tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg cgctacgcct
    41101 gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt aatcaacctg
    41161 atttgccgaa tcccgaccat cggcgatgat tgcccccgta atgcgtggtc cttatttccg
    41221 ataagtttac ttgagtcttt agcttcccat ttcagcccac tctcccctgt gtgggctttt
    41281 ttcattggtc tgacccgtcc tgaatagcca aacaatggca atgttccact ttgcagggat
    41341 gataataagg ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg
    41401 aaccccgtac catacaacaa tcctggttgt cgtctgctct tcctgatgag ctaatggcgg
    41461 tttttgatat gagccttaca gcatattttc tgtcaggctt gagtgcgatg ctaaggtaca
    41521 gaaaagcccc cacacaaaca tgatggaatt atgatttttt tcatatgaat gtcaacccac
    41581 acattcacaa aacatctgca tgagatcatt ttttatacac cgcatagctg cgaaaccact
    41641 gctggtgctt ttttaagcga agcgtcgccg atcacaacaa aatatcggcg aaaaaggcgg
    41701 tggaatgcca tttggcatac ggatatacag atgaaatgat tacctgcttt gcgattcact
    41761 ctctggtgga ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt
    41821 ttaacgtgct tatttacagg ataatatctg tcacctaatt cctgcactct attcgatatc
    41881 cattcattta atcgtgcgtc atctctgtat gtttttacgt ccagaacaga gagcaataac
    41941 atcaccagtg attcaggaaa aacagggtgg atatttttta agtgtaataa taatccatgc
    42001 gcaagcgcct gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat
    42061 ttttccacac gcatacactc tttcccttta gggaaaattc tcagcaaagg catatggtgt
    42121 tcaagaagaa tacatttgca atctgttcgc actgaatcga taaactgcac taatttctta
    42181 agcccggtaa agcgtccagc ctgcacattt gctttaatat caagaaacgc atcatcctgg
    42241 tcatataccc aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg
    42301 tgatcagggt aatccatcca gtcataaaat ccagggaaat cattaaactg cacatttatg
    42361 gtgagtgcgt tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt
    42421 ttgtcgtcat cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc
    42481 aaaaccttga atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga
    42541 ttgtttttaa ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga
    42601 gcttttaatg atatcatcgc gatctctctt aaataaaaat aaacgtcgca gataataaag
    42661 ctctcattaa accaggcact atcagaaatc gctcataact aacgtgtgaa gtattgtgta
    42721 ctggagggag ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata
    42781 ttatttcgct ctccttaact aaaaagttaa gtggagagcg aagttaatat gttaaaccac
    42841 agcgtattta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt
    42901 tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct
    42961 ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa
    43021 tgccgccaat catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg
    43081 gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca
    43141 aggtggtaaa atcgtcgaga ttatgtggct cagctactgt attcaaaatg aaatcgaaac
    43201 tcttcacatg agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca
    43261 gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgtgccccca
    43321 tcgcgtgggc cagcttaatc cccatatgtc ccaggccgcc gatgccgacc acgcccactt
    43381 ttttacccgg cccggcctgc cagtgacgta gcggcgaata cgtggtgatc cctgcacaca
    43441 acaaaggagc caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct
    43501 catgaacgac gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggcgtcg
    43561 gcgagttata ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt
    43621 cgcaatgttt acaactgtcg acaatgcagc cgacaccgac cagatcgccc ggcgcatgtt
    43681 tttctacctg atcaccaacg gctaccacac gccccacaat ttcatgaccc ggtacgcagg
    43741 ggtaaaccgt ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac
    43801 agtaagcgat ttcgattttg acatcattcg gtcccggttc acgccgtgtg atatccatcg
    43861 gttcaagtgg ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc
    43921 ctgattagct atgtgtattt ggcgatgcct gatatgtcat cgcggcaaaa cgcgtccatt
    43981 gaatatagcc aatatttaaa ccacccgatc cagccccaac atgccgcgaa cctgaatgaa
    44041 acagagcagc aacgttcccc ataaggccat cgtcaggtag gggcttatac cgagaatatt
    44101 gaagccgctt tcaataattt gcagaagaaa cagcgccagc accataccaa ttatccgccc
    44161 gctgccgccg tccggattaa ccccgccgag tactgccgcg aggattgaca ccagcaagta
    44221 cgattcgcca taagaggcct tagccgagtt gagtttcgac atcatcaaaa atgccgctac
    44281 cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc acccgacgag tgttaatgcc
    44341 ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa atggctttac cgagcggcgt
    44401 tttctccagc aatatccaca gccccagtgc cactgcggca aacaaccaca tcggtagcgg
    44461 aataccgaac cattgcgcat gattcaacca cagcacccat gagggataat tggcaatggc
    44521 gctaccacct gtcaccagaa tatttatccc tttgagcaac gtcatcatcc caagcgttgc
    44581 aagaattggc gacactcgaa taccagcaat caatacgccg ttacataaac cgattatcac
    44641 cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta gcaatacccg gtggatactg
    44701 agtcgccacc cacgccatca ccagtgaaca ggcgttagca gtggctataa ttgacaagtt
    44761 aatgccaccg caaagcatgg tcaccgccat agccaacgcc agaatgccca gcacgggcat
    44821 ttgcgaagcg acggactgga aattacttat cgaccagaaa atctccggca tagtaatgct
    44881 gaacgcggca acagttagca ccagcaggcc gatcaagtaa aactcaacgt tattacgcca
    44941 cgattttttc atagcagact ccggttctga tgctgactcc acgccgtggc actaatgctg
    45001 gcaacgatga tgatgccggt gatcaatgtt tgccagtaag acgagactcc caataaattt
    45061 agcccgtttt gcatcactgc caacaacacc acgccgagca aagtaccggt taacgtgccg
    45121 cgcccgccga gcaaactagt gccgccaagc accaccgcag ccagtactgt cagctcataa
    45181 cccagaagag aatcgggggc gacagtcatc accgtccacg actgcactac acccgcagcg
    45241 ccagacatca atcccatata gccgtagacg aaaagttgca gtttcagcac gctaaagccg
    45301 atgcgactgg ctgattcgcg gttcccgcca agggcataaa ttttgcgccc tacactggtg
    45361 aaattcatga taaacgctgt cagcagcacc accgtaatca tggcgatcag cggcagacca
    45421 aggccatagt catagccatc agcatcggtg tacttgaaca acataacccc ctgctcaaac
    45481 cacggcggaa agtcgtacag ccacacacct ttactcaacc ataacagcag gccatagaaa
    45541 atattcagcg tcgagatagt gatgatgata gaaggcaccc gtaggcgatt aaccagtaag
    45601 gcgttaatta agccaagtaa aatgccgatg cagcccgcca gtgcaaacgc gacagcgaag
    45661 ttgccaccaa ttttttgcag caacagcacc atgccgtatt gcgcaatgat ggtcattgct
    45721 ggaaacgaaa tatcaattcc accggaaatc aacaccacaa acaaaccaca ggcgagaata
    45781 gtcaacatgg catagttatt ggcgagatcg tacaaattac cgaatgtcag aaactcgtcg
    45841 ctgcgccagg caaggccaac aaacagcacc acaatcagta atgccagcca gaattcatgg
    45901 cgttttttca gttcagccat ttaccacctc cgcaatcctg gcctgagaac tgctgtcagg
    45961 caggaagcta tgggtgatct gacctttttg cattaccaaa atccggtggc tttgatacca
    46021 cgcttcttca atttcatcgc agatcatcaa taccgcaatg ccatgagccg ccagatcgct
    46081 gataatgccg tatattcctg ctttgttggc gatatcaaca ccgactgtcg ggctatcgag
    46141 aatcaacagt cgtggcccaa tcgccagcca tttggcgatc gatactcgct gcgcgttacc
    46201 accagaaagc gtattcaccg gaagatgagg atcagagact ttgatggtca atgccttgat
    46261 tagcctgtcc accaggtcac gaactttagt ttcactcaga aagccgaagc gatcttttac
    46321 cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat tgcgccatca ccaaaccgcg
    46381 cgacatgcga tcttccgaaa cataaccaat accgtggcgg atagcgtcct ggttactatg
    46441 cagtgtaacc ggctggccgt tgatgaggat ctcgccagca tccggtcggg tcataccaaa
    46501 cagactcaga cataattccg tgcgccccgc accaagcagg ccaacaatcg ataccacttc
    46561 gcccgcttcc acccgcaagt taatatttag atattcccca tgacgactta aattacgcac
    46621 ctcaagcacc ggggtgctgg attttccctg ccacaactct cgcacctgat attcgaactc
    46681 ctggccggtc atcaggaaac cgagttgttt tgtggtcatc tctgcagccg gaaaagtccc
    46741 gaccagctca ccatctttca gcactgaaat acggtcggaa acttccatca cttcttccag
    46801 acgatgactg acgaagacca cgcagatccc gcgttcccgc aattgatgca ccacctgtaa
    46861 taacccttgc acttcctgat gggtcagcga agcggttggt tcatccatca cgatcaaccg
    46921 tgcgtcctgc gccagtgcac ggcaaattgc taccagctga cagcgggcaa ttgaaagctc
    46981 agaaacgagc gtatccagtg gcaatgtgac gttaatactg gtcattgccg cctgcgccac
    47041 ctcacgcaga cgacggcggt taacaaacag accgtggtga tagtgattca cggcgatatt
    47101 ctcccagaca ctcaggttag gaaataaaga gaggtcctga taaatcacct gaatcccctg
    47161 cgccaccgaa gcggcgggtg tcagctttga ccagtttttc ccaccaagcg taatttccgc
    47221 tccctcatcc gggcgataaa caccggacat aattttaatc aacgtcgatt taccacagcc
    47281 gttttgcccg gcaaggcagt gcacttcacc cgacataaag tcgagagaaa catcgcgcag
    47341 cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg gaaaggaagg tttccataac
    47401 atcccctggc aataagaatc ggggctttcg ccccgaagaa atggattaat aaagtgagtc
    47461 gatattgtct ttgttgacca gtaatacctt atggaaacgg ataattcgct tatccatatc
    47521 tacgtcggcc ttacccagat tctgcatttc taatccaggt ttgatctctt cacccttcag
    47581 caacgtgctg gcgacggctg cgagtgcgta acctgcagag gccggatcgt aagtaatccc
    47641 ttcggtgata tcaccacttt taatcagtga tgccgcctgt gaagggatca tcatgccata
    47701 gactgcgact ttatttttcg cccgtttctc tttcaccgca cgtcccgcgc caatcggacc
    47761 gtttgaacca aaggagacaa ccgctttcaa gtcaggatag gttttcatca ggtccagtgt
    47821 ggtacgacgt gagtcatcca cactctcggc aaccggcatg cggcgggtaa cttcatgcat
    47881 atccgggtaa tgctctttct ggtatttcac cagcaagtca gcccataagt tatgctgcgg
    47941 cacggtcaaa ctacccacgt aaatcacata gccgcccttg ccccccatgc gtttcgccat
    48001 atgctcaaca tattcagcgg caaatttttc gttatcaatg atttcgatat cccagttagc
    48061 acttggctga ccgggggatt cgttggtcag aaccacaatt ccggcatctc gcgctttttt
    48121 gaataccggt tccagcacgt tggcatcgtt tggcacgata gtaattgcat taaccttacg
    48181 ggcgattaaa tcctcaataa ttttaacttg ttgcggagca tcagtacttg aaggccccac
    48241 ctgtgacgca ttaacaccaa aggcgttacc cgcctcaacc acaccttcgc ccatgcgatt
    48301 aaaccacggc ataccatcga ctttagagat attcaccacg actttttccg ctgcctggag
    48361 cggcgcagaa attagcgcag cgcctaataa cagcgaagac accatattga taacaaaacg
    48421 tttattcatc atttcagcct cattgcaggg tagtcaggaa gtgttcgccc aaattagcgc
    48481 gctggagaga ataagaattt tcgaatattt ttatgcagaa taaacagccc tatttcaggc
    48541 agctattaaa aatatggtta taaatatgga caaaacgggc agcaacaata agtgtgacat
    48601 tcatcacgta aaaaatagca aaataaacaa aattagcgat attttatgtg tgtgagttta
    48661 tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct tcaaatataa
    48721 cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt tcattacgcc
    48781 aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg gtgacatcag
    48841 aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca ccacggctat
    48901 tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata catcccgcca
    48961 gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa cgttgcgagg tgatcggcca
    49021 acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt gagaattttt
    49081 cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat cttaattact
    49141 gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt tttatccgaa
    49201 gcaggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg tgtatttctc
    49261 tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca ttcattagca
    49321 aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt tgcaggcgtt
    49381 tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg gaacgttgcg
    49441 gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaag
    49501 ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag cggcaataca
    49561 tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg aatatagagc
    49621 tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga ccagtggtcg
    49681 ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg tttgagcggc
    49741 atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc cgccatccga
    49801 ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc gtggctgatg
    49861 gtcagcttac ctttcagttg cggcgttttc tctaccgttt caaccatata attgatggct
    49921 gccacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc gtagtccagc
    49981 gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcgacact ggtcgggtcc
    50041 agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg ttccgatttc
    50101 gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc cgcctgacgt
    50161 cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg ttcgatattg
    50221 cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag tttttctgcc
    50281 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt gatcatgtcc
    50341 tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
    50401 tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca acttaccgcc agcgtcatag
    50461 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcgacaat tttgccgtcc
    50521 tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg ccacaccgtt ttcgtagtca
    50581 aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg
    50641 ttgttcgctt cgcagtttag ggtgccagct accgccgcat gggcgagcgg cacagaggta
    50701 ccaaacagcg cggcggcggt gaccatctta ccgctctggc tcagaaattc acggcggcta
    50761 ttgctttctt tcatcctacg tccttacgtt ttaataatat gggtgcccgt ttcaccgcgc
    50821 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ctgctccagg
    50881 aacgtcaggc tggcgatgat ttttggcaac atgctgccgg gcggaaaatg cccttcctgc
    50941 atatagcgag tcatggtggc aatatccacc cgatccagcg cctgctgctg cggtttacca
    51001 aagtggatac agactttttc gacgccggtg gtgatcacca gaatgtcggc gtgaatttca
    51061 cgggccagca gcgcggtaga gagatctttg tcaataaccg cgtccacgct ttggtaatcc
    51121 cccgcttcag tacgcactac cggaattcca ccgccgcccg cgccaattac gacaaaacct
    51181 tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc cggcgaggcg
    51241 accacgcggc gatagccccg tccgacatct tcaacaaaac accagtcagg gtttgccttt
    51301 tgtagtttgt cacgtgtaac caggtaaatc atttgatacc gacaagggtt ttagtgaaag
    51361 gaggtgaaat cacctgtaag atactgaaat aaaacaggtg atcgatgatt ctggaggttg
    51421 tttagttttg atactgatta aataaaatcc ggtataaaat taaggtatcg gcaacaaatt
    51481 tgatactgca catactgctt atggacgttt ttaaaagtac ttaaaatgtc ttttatgcta
    51541 aaaagtttat atcaatatcg atctggtcac aaatcaagtt gaaagtgctc gctaagtgca
    51601 tggcctgggc ttcatggggt tacgctttgg cttctttatg ggttaaattg aaatgatgat
    51661 ccttggtttc atctccctcg aaaatctctc taaccttttg atctgataaa ttattaaggc
    51721 tcaatgctgc caggcgtcga atacctgtta aagcgatcaa ctcttgattc tcttgattgc
    51781 agaattccag caatttttct acccctttcc ttctcaaggt gtaaataact aattcttgct
    51841 cttcttggct caaagtgtcg atgagttcga gcaactcgct attgccaccg ttcttttgag
    51901 ggggctggtg ttttatttct gcactacctg taatgatgga aacaacatgt gcaggtaagt
    51961 cgcttaaacg gtaggcaaat gctctccctc tgataccagg tgtagttacc gaacgttttt
    52021 cccatccttc attttttgct cgtttatgaa gcccagccag agatgaagga agacctccaa
    52081 cccccaacaa gtcactggca aggatccatt cttggtcaag gatcatatca aactccattt
    52141 gatattaact tttgttcata tcttttatgt ggtttattcc aaaaataact caataaaaaa
    52201 caaatagata cagagcaaat ttaaaaattt gaaattaacc agttgatatt cactaagtta
    52261 atatctaaca tatcaaacat acactagtga gtaactcaat agagtagcac acatgagtaa
    52321 taaagtttgc catagaaagt acacaggggg ccattgtgag tcttattgaa gattggtctt
    52381 ccgccgatat aaaggcagcc ttagaaaaac gcgataccaa tttgtcacgt ttatcaaggg
    52441 aaaataattt gcagcctagg actttgggta atgctttgcg agttccctac cctaaaggtg
    52501 aaaagataat tgctagggct ataggtgtag agccacaggt tatttggcct actagatatg
    52561 aacgacgtgc gcggaggaaa tatgtgggtt gcaatgactg aattgatttc attcagtgac
    52621 ctacctagtt ctttggcagg tctgcacaaa aaagcaaaga gggaagcgtg gaaaacgcgg
    52681 ctaaaaccag gggtaaaagg gaaagtttta gagtgtgaaa taggagcttt gcctttaact
    52741 gttcagcagg cagtacgcga acgctatgct ctgcaactga tgacgcaaaa agccgatgaa
    52801 tcaccggctc cggtagtgac aaaggccagg cgctc
//
LOCUS       DAZUFB010000023        49690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZUFB010000023 DAZUFB010000000
VERSION     DAZUFB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.023"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..712
                     /gene="comR"
                     /locus_tag="ACHY3M_001561"
     CDS             80..712
                     /gene="comR"
                     /locus_tag="ACHY3M_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFS4316497.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(774..1313)
                     /gene="ycfJ"
                     /locus_tag="ACHY3M_001562"
     CDS             complement(774..1313)
                     /gene="ycfJ"
                     /locus_tag="ACHY3M_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS4316498.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1540..2844)
                     /gene="ndh"
                     /locus_tag="ACHY3M_001563"
     CDS             complement(1540..2844)
                     /gene="ndh"
                     /locus_tag="ACHY3M_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFS4316499.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3244..3786)
                     /gene="ycfP"
                     /locus_tag="ACHY3M_001564"
     CDS             complement(3244..3786)
                     /gene="ycfP"
                     /locus_tag="ACHY3M_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFS4316500.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERLLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="ACHY3M_001565"
     CDS             complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="ACHY3M_001565"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFS4316501.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="ACHY3M_001566"
     CDS             complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="ACHY3M_001566"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFS4316502.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="ACHY3M_001567"
     CDS             complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="ACHY3M_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFS4316503.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6305..6682)
                     /gene="ycfL"
                     /locus_tag="ACHY3M_001568"
     CDS             complement(6305..6682)
                     /gene="ycfL"
                     /locus_tag="ACHY3M_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFS4316504.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMGSSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="ACHY3M_001569"
     CDS             complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="ACHY3M_001569"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFS4316505.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7378..9567
                     /gene="fhuE"
                     /locus_tag="ACHY3M_001570"
     CDS             7378..9567
                     /gene="fhuE"
                     /locus_tag="ACHY3M_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFS4316506.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="ACHY3M_001571"
     CDS             complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="ACHY3M_001571"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFS4316507.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11355..12152)
                     /gene="ycfH"
                     /locus_tag="ACHY3M_001572"
     CDS             complement(11355..12152)
                     /gene="ycfH"
                     /locus_tag="ACHY3M_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4316508.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12163..13167)
                     /gene="holB"
                     /locus_tag="ACHY3M_001573"
     CDS             complement(12163..13167)
                     /gene="holB"
                     /locus_tag="ACHY3M_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4316509.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13164..13805)
                     /gene="tmk"
                     /locus_tag="ACHY3M_001574"
     CDS             complement(13164..13805)
                     /gene="tmk"
                     /locus_tag="ACHY3M_001574"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4316510.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="ACHY3M_001575"
     CDS             complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="ACHY3M_001575"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFS4316511.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="ACHY3M_001576"
     CDS             complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="ACHY3M_001576"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFS4316512.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMSVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="ACHY3M_001577"
     CDS             complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="ACHY3M_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4316513.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="ACHY3M_001578"
     CDS             complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="ACHY3M_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4316514.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="ACHY3M_001579"
     CDS             complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="ACHY3M_001579"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFS4316515.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="ACHY3M_001580"
     CDS             complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="ACHY3M_001580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4316516.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="ACHY3M_001581"
     CDS             complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="ACHY3M_001581"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4316517.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="ACHY3M_001582"
     CDS             complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="ACHY3M_001582"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4316518.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="ACHY3M_001583"
     CDS             complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="ACHY3M_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4316519.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="ACHY3M_001584"
     CDS             complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="ACHY3M_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFS4316520.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22334..22918
                     /gene="yceF"
                     /locus_tag="ACHY3M_001585"
     CDS             22334..22918
                     /gene="yceF"
                     /locus_tag="ACHY3M_001585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFS4316521.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="ACHY3M_001586"
     CDS             complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="ACHY3M_001586"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFS4316522.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24562..27747
                     /gene="rne"
                     /locus_tag="ACHY3M_001587"
     CDS             24562..27747
                     /gene="rne"
                     /locus_tag="ACHY3M_001587"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFS4316523.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27943..28896)
                     /gene="flgL"
                     /locus_tag="ACHY3M_001588"
     CDS             complement(27943..28896)
                     /gene="flgL"
                     /locus_tag="ACHY3M_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS4316524.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28908..30551)
                     /gene="flgK"
                     /locus_tag="ACHY3M_001589"
     CDS             complement(28908..30551)
                     /gene="flgK"
                     /locus_tag="ACHY3M_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFS4316525.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGALGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHY3M_001590"
     CDS             complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHY3M_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFS4316526.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKEGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHY3M_001591"
     CDS             complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHY3M_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS4316527.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHY3M_001592"
     CDS             complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHY3M_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS4316528.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHY3M_001593"
     CDS             complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHY3M_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS4316529.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHY3M_001594"
     CDS             complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHY3M_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFS4316530.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHY3M_001595"
     CDS             complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHY3M_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS4316531.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHY3M_001596"
     CDS             complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHY3M_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFS4316532.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPKGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHY3M_001597"
     CDS             complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHY3M_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS4316533.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHY3M_001598"
     CDS             complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHY3M_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFS4316534.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38029..38688
                     /gene="flgA"
                     /locus_tag="ACHY3M_001599"
     CDS             38029..38688
                     /gene="flgA"
                     /locus_tag="ACHY3M_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS4316535.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38764..39057
                     /gene="flgM"
                     /locus_tag="ACHY3M_001600"
     CDS             38764..39057
                     /gene="flgM"
                     /locus_tag="ACHY3M_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS4316536.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39062..39478
                     /gene="flgN"
                     /locus_tag="ACHY3M_001601"
     CDS             39062..39478
                     /gene="flgN"
                     /locus_tag="ACHY3M_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFS4316537.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHY3M_001602"
     CDS             complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHY3M_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFS4316538.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41163..42086)
                     /gene="yceM"
                     /locus_tag="ACHY3M_001603"
     CDS             complement(41163..42086)
                     /gene="yceM"
                     /locus_tag="ACHY3M_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4316539.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42088..42735)
                     /gene="yceH"
                     /locus_tag="ACHY3M_001604"
     CDS             complement(42088..42735)
                     /gene="yceH"
                     /locus_tag="ACHY3M_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFS4316540.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHY3M_001605"
     CDS             complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHY3M_001605"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4316541.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHY3M_001606"
     CDS             43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHY3M_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFS4316542.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44838..45485
                     /gene="grxB"
                     /locus_tag="ACHY3M_001607"
     CDS             44838..45485
                     /gene="grxB"
                     /locus_tag="ACHY3M_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFS4316543.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45619..46179
                     /gene="yceB"
                     /locus_tag="ACHY3M_001608"
     CDS             45619..46179
                     /gene="yceB"
                     /locus_tag="ACHY3M_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4316544.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDTNLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYILREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHY3M_001609"
     CDS             46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHY3M_001609"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4316545.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47404..47649
                     /gene="dinI"
                     /locus_tag="ACHY3M_001610"
     CDS             47404..47649
                     /gene="dinI"
                     /locus_tag="ACHY3M_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFS4316546.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47939..48193
                     /gene="bssS"
                     /locus_tag="ACHY3M_001611"
     CDS             47939..48193
                     /gene="bssS"
                     /locus_tag="ACHY3M_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFS4316547.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48308..49426
                     /gene="solA"
                     /locus_tag="ACHY3M_001612"
     CDS             48308..49426
                     /gene="solA"
                     /locus_tag="ACHY3M_001612"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFS4316548.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49474..49599
                     /locus_tag="ACHY3M_001613"
     CDS             49474..49599
                     /locus_tag="ACHY3M_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFS4316549.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYFSFPH"
BASE COUNT        12059 a        13385 c        12316 g        11930 t
ORIGIN      
        1 tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt cattcacagt
       61 caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg
      121 ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat tgttctggca
      181 acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc
      241 cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta
      301 tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga aaagcgtgga
      361 gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca aagacacccc
      421 ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag gagatatcgc
      481 caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt ttttatgtca
      541 acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg cagaattcct
      601 taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact
      661 aatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat aagtcgcaaa
      721 aaaagcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc ttgttatact
      781 ttgttattca aaatcagttg cccattacta tctagcggga tctgcgtacc cggatcgcgg
      841 tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata accgagcatt
      901 ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt
      961 tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc ccccacaaca
     1021 gtggcgacat ctttaccgcg accaccgcca aactgatgcc ctatcacgcc gccagcaaca
     1081 gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg atgggtcact
     1141 gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa
     1201 acaacctgag cgtattgcgg gccacgttca aacacgttca gactggccac tgccgctacg
     1261 cccagcgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt cacgggaaca
     1321 cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc tatcagacag
     1381 aagatgaaaa gcgcgggaat ggctggaaat acttaatgat tcggaggagt ctgaaggaat
     1441 gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc
     1501 ggatgaggcg tttatgccac atccgccagt gtacgtcgat taatgcaact tcaaacgcgg
     1561 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
     1621 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
     1681 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg agaagttcga
     1741 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct taccattcat
     1801 ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg
     1861 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
     1921 tggatcgcgg gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttagtttc
     1981 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc acaccatcag
     2041 atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag cactggtgac
     2101 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
     2161 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
     2221 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa
     2281 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc
     2341 atttgcgccc aggttggcgg agtatttcag gaagagattc agcatctcct ggtgaaagcg
     2401 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacacctg gcgtattgaa
     2461 atcgttagag gtgctaccca gcgccattac cagggtgtca taggcgattt tacgttccgg
     2521 aaccagcagt tcacctttct catcgcgcag ttctgcaata gtgattgttt tcgcttcacg
     2581 atcaatatca atgacggaac ccagctggaa ctggaaacca tgattgcgcg catgggccag
     2641 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagcgg
     2701 tttccacaga tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttgcgtcc
     2761 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
     2821 aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
     2881 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
     2941 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
     3001 ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa aacagagttt attgtttatt
     3061 atttgagcaa gtccggaagg gcgatatagg gtgaagcgta tatcgagggc gtttggtcgt
     3121 cctgataaga ggcgcatcag gcattgatgc tcgaatgtcg gatgcggcgt gaacgcctta
     3181 tccgacccac agaatccgga gcgcatcagg taccggcaag gttacctgat gcgacgaggg
     3241 catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa
     3301 tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcagct cttcagatgt
     3361 ccgctggctg ttaagcgctt catcattacg cgacaaaatc accaggcaac gatcgcgatt
     3421 cttctcacgg aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc
     3481 aatcttgcct tccatgttct cataagggaa caaattaggg ttgaagatca cctggcggat
     3541 atcgcaaaga aaaccaatcc gttccgccca gtatccgccc agaccaacgc cgcaaatcag
     3601 cagacgctcg tcgacgttca gttgcaacat tttgtccact tctttgagca gatgctgcat
     3661 atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt caataaactg
     3721 caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt gtaaatagat
     3781 aatcatcgct tcctcacata agccagggtt agtgacctgc tttctcttcc tgccagcgtt
     3841 catgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc atcagttcct
     3901 gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca
     3961 gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac
     4021 ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg gcaccttcca
     4081 tcgataaatc gtcagagaaa atcacgccgt caaaacccag ttcctgacgc aaaacggttt
     4141 tcagccagta gggagaaccg ctcgccggac gcggatcaac atcactgtag atcacatgcg
     4201 caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt
     4261 tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt gagtctgccg
     4321 ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct tcatgcatac
     4381 catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga taagaacgct
     4441 cgccaatcgc cgcgctgata tgcccgacat ccagcactgg cgcaaagctg atatcaatat
     4501 ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt ttgccgccct
     4561 cttccattcc tgacagcgca gcgaatgatt gcgccgctgg caagcgggta aaaccttcac
     4621 gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga ttgcgcgaag
     4681 ctgcgcggat ctggcgcacc agttcacgca actgggcagg atcatgatag ttacgcgtaa
     4741 agagaatcag ccctcccacc agcggatgcg caagtatttc acgctcttcc gcgtccagtt
     4801 cgtaaccttc gacatccaac attactggac ccacactgct ctccttattg ttttattaat
     4861 agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag
     4921 cggtactcaa accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga
     4981 cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccgg
     5041 tgctgttcag tattttccac ccacactgcc gccagttcca gcgcgatatc accatctccg
     5101 gcatactccc agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg
     5161 acatccatat gcaatggact taagcgtaaa ggccgtggtt cccgcgcttt gcgcagacgt
     5221 tttaacattc gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt
     5281 tccagtaacg gcaacagcgt tattcgccag ccaaaacgtg gttgttgatg tagataatac
     5341 agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttacctcgcc gggcagatag
     5401 tcgactacca tccagtcacg gagatataaa tgcggcttcg gtgcaatgct tgcgggtagt
     5461 tgtgataaag cccgatactg gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc
     5521 tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa aactcccgcc actaagccca
     5581 ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc
     5641 atgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg
     5701 caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg cgctggagta
     5761 cagcacgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac gggtacctaa
     5821 actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagctgct gggcggaaac
     5881 cagggtaaat ttcccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc
     5941 attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt
     6001 caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata
     6061 atggcgaata tgcggcgcag gcggtgccgt ttgatcttcg tgctcaattg ggcctggctg
     6121 ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct cggctggttg
     6181 ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc
     6241 gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca
     6301 ccccttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6361 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     6421 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     6481 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6541 tttctgcact gatgcccgca gccaataaag atgaccccat caccagcgat tgctcatcat
     6601 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacgcc agagacacca
     6661 gcccaaagca tccttttctc atcgttacag acctttatgt gccagcattg gtcccagcgg
     6721 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6781 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6841 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     6901 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6961 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     7021 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     7081 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     7141 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7201 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgtcaaata
     7261 tggatgcgta tatttctcat ttgcatttac atacaaaatt attcgcacac taaatataac
     7321 cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag atttcaaatg
     7381 ctttcaacac aatttaacag ggataatcaa tatcaagcca gcaccaaacc atcactactt
     7441 gccggttgca tagcattggc actattacct tctgccgctt ttgccgctcc agccactgaa
     7501 gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa tgattacagc
     7561 gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat tcctcagtcg
     7621 gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg
     7681 atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat
     7741 tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg
     7801 cgctggaatc tgggcgacgc actttctgat atggcactct ttgaacgcgt agaagtagtg
     7861 cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt
     7921 cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg
     7981 aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc
     8041 gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta caacagtgaa
     8101 aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac gctttcagcc
     8161 ggttacgaat atcagcacat tgatgttaat agcccaacct ggggcggttt accgcgctgg
     8221 aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc tgactgggcg
     8281 tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc
     8341 tggcaagcga cgctgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat
     8401 gtcgatgcct atgtaaacaa agcggatggt atgctgattg ggccatacag taattatgga
     8461 cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg
     8521 ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt
     8581 ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa catcttcccg
     8641 gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga ctggtcacca
     8701 cagagcctgg cgcaggacga taccacacat atgaaatcgt tatatgctgc cactcgtgtc
     8761 acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg gcgggttgat
     8821 acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct ggtgtttgac
     8881 atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc gcaaaatgat
     8941 cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga gctgggtctg
     9001 aaatcggact ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag
     9061 gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat
     9121 aaagcagtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg cgcaattacc
     9181 gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa cgaaggaaac
     9241 gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct
     9301 gtcatgccag agttgacagt cggcggtggt gttaactggc aaaatcgcgt gtataccgac
     9361 accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct ggtggatctc
     9421 ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc
     9481 gacaaaacct acgataccaa cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc
     9541 agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc catctggctg
     9601 ccttagtctc cccaacgtct tacagattag tggttacgga tgtactcatc catctcggtt
     9661 ttcaggttgt cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg
     9721 cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg
     9781 acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca
     9841 gcaaccagag ccggtgccat ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct
     9901 tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa
     9961 acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac
    10021 agaacgatga agtcgatcag accgtgcgag aacgacgtac cgtcacgcat ccccagaaga
    10081 atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca
    10141 acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc
    10201 gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata
    10261 gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc
    10321 gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg
    10381 ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac
    10441 ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa
    10501 gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat gaaggacagc
    10561 acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg tttaccggca
    10621 aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg
    10681 atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt agaggcgatt
    10741 tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg
    10801 ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa gccgagggcg
    10861 acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata
    10921 acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata
    10981 cctgcgatag gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta
    11041 gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt
    11101 ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt
    11161 tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac gtttcaactt
    11221 cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg
    11281 ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta attacgagct
    11341 ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg aaacagacgg
    11401 gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc
    11461 atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtacc
    11521 ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac ataacgcgca
    11581 gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat
    11641 ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca
    11701 ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg ggcgtcacgg
    11761 gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac
    11821 tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt ttcacccagc
    11881 gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc
    11941 tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc taccagatcc
    12001 cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt
    12061 gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca
    12121 tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg
    12181 tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag
    12241 ctcgcggttg atgcctgtaa cagacattaa ctgttcacga atgtggcaaa catcccccag
    12301 tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca ggcccggcac
    12361 atcaacattg gtcacctgcg cagcaccatg atggcgtttt agcgcatcca tcagcaacgt
    12421 tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcta gcagcgaata
    12481 ccagtcgccc gatggcacgc tatatgccaa cgcctgacac aatgtttcac gagcctgcca
    12541 gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc
    12601 ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg
    12661 ttccggcggc ggcgcaagat aatgtaaccg acaacgacta cgtaatgttg ccagtaaacg
    12721 ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt caagcgtttt
    12781 cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc agacaacttt
    12841 cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat
    12901 gcccagcata tttttccctt tttcgggagc cagggtgtag taatcgggat gcgtgccagc
    12961 ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca
    13021 gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg
    13081 aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc
    13141 aggtcgtaac catggatacc atctcatgcg tccaactcct taacccagtg ggtcacggta
    13201 gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatactt
    13261 ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac
    13321 tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg
    13381 gtaacatcga gatagagcgt taagtcgggg cgaaaatccc cgagaacagc atcacgcagt
    13441 gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg agtggagaga
    13501 tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat gaccgtttct
    13561 accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgacttca
    13621 tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg
    13681 ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt ctcaaccacc
    13741 acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg
    13801 cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat ggctggcaag
    13861 attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt
    13921 tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg cacccggcgg
    13981 caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag
    14041 tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct gcaggcgcat
    14101 accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac tggcaacggc
    14161 ggtttctttt tcgataattg atgccatcgt caccagctga tttttatctt tataaggcag
    14221 accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg
    14281 cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca
    14341 accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc
    14401 gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag
    14461 acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt
    14521 cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga
    14581 aagatccggt tcgatacgca gcagccattg aaaaacccgt gggcgattga tgatcttatc
    14641 agcataaagc tgttcaccga gcgccagacg tccggtccct ggcttcaggg taaatatcgt
    14701 ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc
    14761 agcggcgata cccagtacca ccagcaataa caagataatc aataacactt ttttcatgac
    14821 taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag
    14881 acatcgccac aggcacgtac ggacatcact ggcattaacg cattacaaat aaccatctca
    14941 tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt
    15001 gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc
    15061 ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc
    15121 cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcgg
    15181 atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgagg attgcgcccc
    15241 agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc gtaatgtgca
    15301 ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct
    15361 cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg ttctgctgcc
    15421 agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat caaccgctga
    15481 caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact
    15541 ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat
    15601 tcctgcttat aaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg
    15661 gcagcatgtt cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg
    15721 accttttata tgggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag
    15781 tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc tgacgtgctt
    15841 catgcggtac gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg
    15901 gaacagcctg atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac
    15961 ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac
    16021 cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt
    16081 gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca
    16141 ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc
    16201 tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt
    16261 actcttcaag taccagcata ccggcaccat cgcccagtac gaagccatca cgctctttat
    16321 cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc
    16381 caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt
    16441 cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg ccggaagtac
    16501 aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg
    16561 ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt
    16621 tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg
    16681 cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct
    16741 gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg
    16801 agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc
    16861 tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg
    16921 tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac
    16981 gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa
    17041 aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt
    17101 aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat
    17161 caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt
    17221 cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga
    17281 taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg
    17341 ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca
    17401 ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt
    17461 gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc
    17521 gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg
    17581 cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac
    17641 ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt
    17701 ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc
    17761 gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac
    17821 cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt
    17881 tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga
    17941 taatttcgtt ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg
    18001 cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt
    18061 cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca cctaaataat
    18121 cgctgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg
    18181 ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga
    18241 tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg
    18301 aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa
    18361 gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa
    18421 cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca cggatggcat
    18481 caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt ggtgcgttaa
    18541 aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt
    18601 gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct ttacaggcag
    18661 cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa
    18721 agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc gcaatagacg
    18781 catcatccag accgatgatt gccgccatag cgccagtgcc ttccggtacg gcttcttgca
    18841 tgaacttacc gcgcatctca accagacgca ccgcatcagc gaaatcaatc acgccagcgc
    18901 aaaccagggc ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc
    18961 cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct ggctgggttt
    19021 gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc
    19081 ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat
    19141 cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca
    19201 tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa
    19261 aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca
    19321 gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat
    19381 tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat
    19441 gcggaaccag ccagtccagt tgagaacggt caagattatt tgccgccagc gtctcatcaa
    19501 cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat
    19561 gaattgaatt ctctggattc acacggtcag cattaggcag cgtcagcaac tcaccatagc
    19621 taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca gccagcaccg
    19681 cagcgcccgc gccatcgcca aaaataataa tagtcccacg atcggttgga tcgcaggtgc
    19741 gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt
    19801 gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg
    19861 ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg
    19921 tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct
    19981 caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggtgcggcaa
    20041 tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt
    20101 tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct
    20161 tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt
    20221 tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa
    20281 ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc
    20341 gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt
    20401 tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg
    20461 tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac
    20521 gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct
    20581 tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg
    20641 ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta
    20701 agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc accaatattg
    20761 agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac tgagcccata
    20821 atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa atcgaggacc
    20881 accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg ctcaatcccc
    20941 tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg
    21001 acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat tgaactccca
    21061 cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga
    21121 ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg
    21181 tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct
    21241 gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga
    21301 cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct
    21361 cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt
    21421 aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca
    21481 ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg
    21541 gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa
    21601 cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt
    21661 gcgcttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag
    21721 aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca
    21781 gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag
    21841 cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg
    21901 gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg
    21961 cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga
    22021 ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg taatcaaggc
    22081 gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc
    22141 gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt
    22201 tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga
    22261 acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct
    22321 gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc gtcgcgctct
    22381 tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg aaaccccacg
    22441 cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct
    22501 ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg
    22561 cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag
    22621 cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga atgggcatct
    22681 acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga
    22741 taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt
    22801 tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg ttggtttacc
    22861 gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga tgggatagtt
    22921 gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc tgatgcggcg
    22981 taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat tagcgcgcgt
    23041 tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg atacgcatca
    23101 cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac agacgattta
    23161 atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag cgatcgtcaa
    23221 aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta cgtccggtta
    23281 ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta aagcgtgttt
    23341 ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc agaatatttt
    23401 tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca
    23461 ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc aacgccgagc
    23521 gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga accagttcaa
    23581 ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag cttaaaccac
    23641 tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga tcatcttcat
    23701 ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt
    23761 cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg agtttatatt
    23821 caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata cggtaaatca
    23881 tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt
    23941 cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg gtgggcgatt
    24001 ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta gttttacacc
    24061 ttattacttc actacgtgat cactttattg atggttatta aaccaatcac cagcaagaag
    24121 tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct
    24181 tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg
    24241 aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt
    24301 tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag
    24361 agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa
    24421 ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca
    24481 cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cacagcttag tcgtcaatgt
    24541 aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag
    24601 ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg
    24661 cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg
    24721 gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt
    24781 gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg
    24841 ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc
    24901 gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat gccgaacaac
    24961 ccgcgcgcgg gtggcatttc tcgccgtatc gaaggcgacg accgtaccga attaaaagaa
    25021 gcactggcga gccttgaact gccggaaggc atggggctta tcgtgcgcac cgctggcgtc
    25081 ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gtctgaaaca ctgggaagcc
    25141 atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta
    25201 atcgttcgcg cattccgcga ttacttacgt caggacatcg gcgaaatcct tatcgataac
    25261 ccgaaagtgc tcgaactggc acgtcagcat atcgctgcat taggtcgccc ggatttcagc
    25321 agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg
    25381 cagatcgaat ccgccttcca gcgtgaagtc cgtctgccgt ccggtggttc cattgttatc
    25441 gacagcaccg aagcgttaac cgccatcgac atcaattccg cacgcgcgac ccgcggcggc
    25501 gatatcgaag aaactgcgtt taacaccaac ctcgaagctg ccgatgagat tgctcgtcag
    25561 ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgcctgta
    25621 cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgtgcgcgt
    25681 attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgtca gcgcctgagc
    25741 ccatcactgg gtgaatccag tcatcacgtt tgtccgcgtt gttctggtac tggcaccgtg
    25801 cgtgacaacg aatcgctgtc gctctctatt ctgcgtctga tcgaagaaga agcgctgaaa
    25861 gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta cctgctgaat
    25921 gaaaaacgtt ctgcggtaaa tgccattgaa actcgtcagg acggtgtgcg ctgtgtaatt
    25981 gtgccaaacg atcagatgga aaccccgcac taccacgtgc tgcgcgtgcg taaaggggaa
    26041 gaaaccccaa ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg
    26101 tctgaagaag agttcgctga acgtaagcgt ccggaacaac ctgcgctggc aacctttgcc
    26161 atgccggatg tgccgccagc gccaacgcca gctgaacctg ccgcgcctgt tgtagcccca
    26221 gcaccgaaag ctgcaccggc aacaccagca actcctgcac aacctgggct gttgagccgc
    26281 ttcttcggcg cactgaaagc gctgttcagc ggtagtgaag aaaccaaacc gaccgagcaa
    26341 ccagcaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag
    26401 aacaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac tgaaggcagc
    26461 gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcagac tgccgagacg
    26521 cgtgagagcc gtcagcaagc tgaggtaacg gaaaaagcac gtaccaccga cgagcagcaa
    26581 gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa
    26641 gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta
    26701 cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa
    26761 agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc tgccgaacca
    26821 attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct gccagtcgta
    26881 gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc
    26941 atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca gcgtcgtcgt
    27001 cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcgcg
    27061 tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat
    27121 gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat ggtgactgag
    27181 gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga agaaatggct
    27241 gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt tgaaacgacg
    27301 catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac cgagtctgat
    27361 gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc gcaggaagag
    27421 acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga acctgaagtt
    27481 gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga aacggtagcc
    27541 gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat gacgcgcgct
    27601 ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg ccctactttt
    27661 gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc ctctgccgct
    27721 cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc cctggtaact
    27781 gccagggctt ttttatttca tctttgaatc aacatattaa aattcatcaa atggcatcct
    27841 tgctaaccaa caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc
    27901 agacaaaaca tacccagttt catgatatgt tttaaagcga gcttatttgt tgagctggaa
    27961 gagcgacaat ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg
    28021 catgatgtaa gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg
    28081 cgtttgccct aaagcgcgat cgctacctaa tgaatccagc gactccagtt cgttcagctg
    28141 cgtgcctaat tccgcgcgaa ctgtaaggac attattcagc gagtttttca gtccacggtt
    28201 ggttttatcc aatgccgcag cggcgatttc tttatccgct tcgctatccg cgaccggcgt
    28261 tttcagggct gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc
    28321 gtctggttcc gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc
    28381 tatcaccatc gaacgcgaag catcgacctg ttgcctaata ctttctgctc caccgacgta
    28441 tttccctttc tcttcgctaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg
    28501 cccgttaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctggatatc
    28561 cgtagccagc gaggcccggt catcgtcact caaagtgcca ttgctggcgt agacaatttt
    28621 ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac
    28681 tttttgagtg gcgaaagtac gcgccagcgt gtactggctg ttttgcgcct gtgcctggga
    28741 gagaacaacc gcttgtgatg ctgcaatggg atcgtcagaa gggttaacga ctcgcttacc
    28801 tgtcgacatc tgctcgccgt acttcatcca ttccgcctga gaattggtga tgccacgcat
    28861 gttttgctgg tacatcatct gggtactgaa acgcatctta tctcccctta gcgaatgtta
    28921 atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac
    28981 tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc
    29041 tgctgattgg aaagctgcgt caccacatta ccttgcgtgg cgctactggt tttcaacgtc
    29101 gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca
    29161 ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt gcggttatcg
    29221 ctatcacccg catcttcttc gctcgccatc gctattttcg cttcgtcggt gattaataca
    29281 tccatgttga cgatagcgtc acttactggt ttcagcgtga agctgtcgtt aacagcaggc
    29341 gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc atccggcgtc
    29401 accgtaaaag tggtattact ggcaaggcgg gtgacctgcc actgattatt atcgaacgag
    29461 attttgtaat cagtcgccag taccgcggag gcatcagtta ccgtggcacc gatcgcaacg
    29521 tcacctgtgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagcaaa gaaatcttca
    29581 ccagcatcgc cgttggcatc aaatccggct ttgtgttggc tgttgaaagc ctcggcaaat
    29641 gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga acggaatgtc
    29701 agaatgccgc ccagcgcccc ggtattcagt aatttctccg ggatctcaat attgcctgcc
    29761 gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg aactgccgcc
    29821 agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggccatcgt gatgttataa
    29881 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    29941 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    30001 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    30061 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    30121 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc
    30181 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    30241 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcagtcagg
    30301 ccgctacttt gcgtctgcgc cgcacgtaac tggttggtaa taaaagcatc atactcacgc
    30361 tgcacaccag aaacgtagac accattgcca acccagccgc cagcgcccaa cgtgctattg
    30421 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    30481 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc
    30541 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    30601 gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct
    30661 tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat
    30721 cggtagcata ccccgcgtcc tgtagggcct gcgccccctg ctccgcactc gcggcggtcg
    30781 tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca
    30841 gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    30901 cagtcgtggt gatttcagta actggccctt tccagttgcc agaggctttg acaccaaaca
    30961 ggttatagct cggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcca
    31021 gtgccgcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    31081 cgggcagcga gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt
    31141 tacgtggcac ggccttttgc accagctgcg aaagcgcctg attttgataa cgcaccacag
    31201 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    31261 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    31321 gggcaatctg ctggtcatac atactggtat acaggcgagt gtgctcgctg ctgaacaggc
    31381 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    31441 cctgacgggc caccggacgg atatttgccg ccggatcttc gccctctttc gcctttagtt
    31501 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    31561 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca gtatggacat
    31621 cagatccatc ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc
    31681 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    31741 agtaaccaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    31801 attgactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    31861 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    31921 attaacctgc atattctgga tatctgcaag gaaacggacc tgggaactgt tgccacttgg
    31981 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    32041 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    32101 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    32161 gatccgtcca ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc
    32221 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    32281 cggcgtcatc aacaacgtac ctccacgcaa gcttttggca tttcccatgg aagaaaccac
    32341 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc
    32401 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    32461 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc
    32521 cagccccacc accagaccat agccaatcag tgagttttgc cttaccccct gaacactggt
    32581 gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga
    32641 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    32701 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca
    32761 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    32821 ccagagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    32881 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    32941 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    33001 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    33061 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    33121 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    33181 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcggc
    33241 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    33301 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    33361 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttaagcctta
    33421 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    33481 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    33541 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    33601 tgcaccagag gattgcgttt cggtgtagag gttttcgcca atgctctcca gcccggtgtc
    33661 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    33721 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    33781 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    33841 gaaagagccg tcacgggtat aggctgatga accatctggc aacatcacct ggaaaaagcc
    33901 ctgcccttta atcgcgacat ctttgctgtt gttggtctgc gacaggtttc cctggctgtg
    33961 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    34021 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    34081 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    34141 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    34201 aaaatgtcct gtagtggatt agtgacaagc agagcagaag cacgcgacag tgtgaatttc
    34261 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    34321 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    34381 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    34441 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    34501 gttggatctg cctgcagtat cggcccacgc gtggcctggc tttctgcgct taaacgaaaa
    34561 atgccgtcgt caccgcgttg cacttcgctg cccgtggctt tcaccagttt cagacgcccc
    34621 actggcgcaa ccgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    34681 gcaatagtga tttccgcccc ttccggcaca gcaattggcc cagcctcgcc tatcaccgga
    34741 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    34801 ccttcgctac cgtcagcgga ctgcacggcc agccagccat cctgctgcaa tgcaacgtcc
    34861 agcgggcgcg aggtgtaatc catcttgccg ggcgtcatgt ctgcacccgg tgttgacgcc
    34921 gttaccagcg tgcgcgtggg cagtgaaagc ccttccaccg gcactgcacg caaggcgttt
    34981 aactgcgcgc gaaaaccggg cgtagaggca ttggccagat tactggcggt taccgcctgt
    35041 tgattcagcg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    35101 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gggttttgat
    35161 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    35221 gagatcgacg ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    35281 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    35341 ggatgccagc ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    35401 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    35461 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    35521 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    35581 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    35641 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    35701 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    35761 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    35821 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gatgccgtgg tggtagtttt
    35881 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    35941 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    36001 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    36061 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    36121 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    36181 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    36241 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    36301 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    36361 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    36421 gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    36481 actggcgcta atcgctacat tgtaagaacc tttcggcgca gtgctgccgt cagtcaacgt
    36541 accgtcccag gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac
    36601 aaccgcacca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    36661 aacaccaaac ggcgtggtcg tggtcacagc tccttcttca ctgccggttc ccgcaagaac
    36721 agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    36781 gttatcaatc tgtccggaaa tagatccgag tgtggtattg agtttttcaa tcccgctgac
    36841 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    36901 ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt
    36961 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    37021 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    37081 atgcttttca ccgtgttgag cacttcaacg ttggcctgat agctgcgtga cgccgacatg
    37141 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatct
    37201 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    37261 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    37321 tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg
    37381 gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    37441 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    37501 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    37561 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    37621 agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga ggtcatcgtc
    37681 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    37741 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    37801 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    37861 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    37921 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatggt
    37981 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctgacaata
    38041 aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc gagcaatctc
    38101 acatcgcaat tgcacaactt ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt
    38161 gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt gctttcgatg
    38221 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    38281 cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcccatt
    38341 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gctggatacc
    38401 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccattag cctgcgcgat
    38461 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    38521 ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag
    38581 gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc gggacaggta
    38641 gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat
    38701 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    38761 accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa
    38821 accactgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc caccagcacc
    38881 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    38941 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    39001 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    39061 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    39121 ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    39181 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    39241 caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    39301 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    39361 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    39421 tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt cgatctgaat
    39481 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    39541 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    39601 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    39661 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    39721 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    39781 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    39841 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    39901 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    39961 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    40021 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa agaatgccca
    40081 acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag tccatcaaac
    40141 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    40201 tgccaagcgc cacgcccagc acaccggacg gaaactccat taagcggtcg gcgtaataca
    40261 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    40321 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    40381 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    40441 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    40501 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    40561 ttggagcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    40621 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    40681 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    40741 cgagcatgcc agcgaccgtc acaaccgcca gcgcgagtgt cagcaggcca gaaacataag
    40801 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    40861 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    40921 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    40981 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    41041 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    41101 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    41161 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcacggcc
    41221 tgttcgccgg cggtttgcgg aactgtctga ttttgcacac attcaatgaa gtgccgcgca
    41281 cagccgacaa atccacgttg ttcaagagtg ctctgccaac caggaatcgg tttatgcact
    41341 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    41401 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaggtggtg
    41461 atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    41521 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    41581 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacactattg
    41641 ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt taactcaccg
    41701 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt acgcgccgcc
    41761 agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    41821 tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    41881 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    41941 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacgtc
    42001 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc acctaatccc
    42061 actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    42121 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct gtaaatcacc
    42181 atcaaccgcg ttcgacatat ccgtcaccgc cggctgatct tcaacctcac cactgaaaag
    42241 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    42301 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    42361 atacattcgc gcggcgcggc tgcgcagttc acctggcgtc tgggcaccac gcaataacaa
    42421 cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca
    42481 aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg
    42541 acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg
    42601 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    42661 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    42721 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    42781 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    42841 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    42901 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    42961 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    43021 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    43081 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    43141 ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    43201 gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca tcacgatcat
    43261 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    43321 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    43381 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    43441 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt tcccgaagtg
    43501 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    43561 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    43621 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    43681 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    43741 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    43801 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    43861 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    43921 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt tttttctcgc
    43981 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    44041 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg
    44101 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    44161 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    44221 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    44281 ctggcgcacc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    44341 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    44401 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggcctgcaac
    44461 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    44521 aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    44581 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    44641 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggcatt attggcatct
    44701 tcactttcct tgcgctgggt tggcagttta gccagaaacg cgccgcgcgt cgtttgcttg
    44761 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    44821 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    44881 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    44941 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    45001 acagccgcta tatgccagaa agcatggaca tcgttcacta tgtcgataaa ctcgacggca
    45061 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    45121 gctacgcaaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    45181 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aatttagccg
    45241 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    45301 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    45361 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    45421 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    45481 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    45541 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    45601 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    45661 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    45721 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    45781 catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa taaggttacc
    45841 ctaaccggag acaccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    45901 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    45961 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    46021 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacatc
    46081 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    46141 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    46201 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc
    46261 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    46321 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    46381 accagcgaaa tttatggacg ggctattgta atgcccaatc tggctccgcc cgtgaccact
    46441 gttgaggctg ccgtggcgta tcgccagcgt attcttgatg ccgtacctgc cgggcacaat
    46501 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    46561 ggatttaacg aaggcgtgtt caccgctgca aaactttatc cggcaaacgc aaccactaac
    46621 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    46681 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    46741 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    46801 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    46861 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    46921 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    46981 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    47041 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    47101 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    47161 gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa cgacacattc
    47221 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    47281 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    47341 cttcctgttg tcattaggtt attttacctg tataaataac cagtatattc aacaggggct
    47401 attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac
    47461 gccctggctg gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac
    47521 gtatcggtac gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat
    47581 aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc
    47641 agcgaataat atgcagtgat ttttgttttt gccgggtcgc cccggctttt ttattttact
    47701 tacaaatttt gagtacaaaa tttctacact tcctcaaaat aattgcacaa tatgctaaaa
    47761 aaatatgaat gacatgctgt tttattgttc atataaatct taaaagcaga aatttatctg
    47821 ttcccccctt taaaaattcg gtgaataccc ttacttattg gtatactgaa gttgcttcag
    47881 ataaaaacgc attgaacctc gaataacgtt gtctagtaac acgaattagg gggccatgat
    47941 ggaaaaaaat aatgaagtca ttcagactca tccgctcgta gggtgggaca tcagcaccgt
    48001 tgatagctat gatgcgctga tgttgcgttt gcactaccag accccaaata agtccgagca
    48061 ggaagggact gaagttggtc agacgctctg gttaaccact gatgttgcca gacaatttat
    48121 ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg taaacgagta
    48181 tcggcgtcat taacatgaca tgaccatcca cccaaaaggc acctcaccgg tgcctttacc
    48241 atttctgggt tgtatatctg cattcagtta attactctgc ttaaaccatg agagcagaga
    48301 gaaaaaaatg aaatacgatc tcatcattat tggcagcggt tccgtaggcg ctgccgccgg
    48361 gtattatgca acccgcgccg gtttaaacgt gctaatgacc gacgcccata tgccaccgca
    48421 tcaacacggc agccaccatg gcgatacgcg attaattcgc catgcttatg gtgaaggcga
    48481 aaagtatgtc ccgctggtcc tccgcgcgca aacgctgtgg gatgaacttt cccgccacaa
    48541 cgaagatgat cccatttttg tacgctctgg tgtcattaac ctcggcccgg ctgactccgc
    48601 atttctcgcc aacgtcgccc acagcgccga acagtggcaa ctcaacgttg aaaagctcga
    48661 tgcgcaaggg attatggccc gctggccaga aatacgcgtc ccggacaact acatcggctt
    48721 atttgagact gattccggtt ttttgcgcag cgaactggcg attaaaacct ggatccaact
    48781 ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg gtcaccgtaa ttcgtcatga
    48841 cgatgatggc gtaactattg aaacggttga cggtgagtat caggcgaaaa aagcgattgt
    48901 ctgcgcggga acatgggtaa aagacctgct cccggagctg cctgtccagc ccgtacgtaa
    48961 agtatttgcc tggtatcagg ccgatggccg ctatagcgtg aagaataaat tcccggcgtt
    49021 taccggtgaa ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt
    49081 gaagattggc aaacataacg gaggccaggt tatccattca gcggatgaac gtgttccgtt
    49141 tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc ttgcgcaatg tattgccggg
    49201 tatcggttgc tgcctgtacg gcgctgcctg cacctatgat aattcgcctg acgaagattt
    49261 tattatcgat accctacccg gccacgataa tacactgctc attaccggcc tgagtgggca
    49321 cggttttaaa tttgcgtcag ttttagggga aatagctgcc gattttgcgc aagacaaaaa
    49381 aagcgatttt gatttgacgc cattcaggct ttcccgcttc caataatcat cattacggcc
    49441 tccagaatga ggccgttttt acaggagtat ttaatgcgtc ggctgttgca ctatctcatc
    49501 aataatattc gcgagcatct gatgctttat ctttttctct ggggattgtt ggcaattatg
    49561 gatctgatct atgtgtttta ttttagtttt ccgcactgac ataaactgac aaatatttaa
    49621 tctccctttc atttggcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    49681 cagacatctc
//
LOCUS       DAZUFB010000024        49472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZUFB010000024 DAZUFB010000000
VERSION     DAZUFB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.024"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..326
                     /locus_tag="ACHY3M_001614"
     CDS             <1..326
                     /locus_tag="ACHY3M_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS4316550.1"
                     /translation="NRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGH
                     TAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEM
                     IQATA"
     gene            370..477
                     /locus_tag="ACHY3M_001615"
                     /pseudo
     CDS             370..477
                     /locus_tag="ACHY3M_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(461..1099)
                     /gene="ytfB"
                     /locus_tag="ACHY3M_001616"
     CDS             complement(461..1099)
                     /gene="ytfB"
                     /locus_tag="ACHY3M_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFS4316551.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1318..1938
                     /gene="fklB"
                     /locus_tag="ACHY3M_001617"
     CDS             1318..1938
                     /gene="fklB"
                     /locus_tag="ACHY3M_001617"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4316552.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2247..3650
                     /gene="cycA"
                     /locus_tag="ACHY3M_001618"
                     /gene_synonym="dagA"
     CDS             2247..3650
                     /gene="cycA"
                     /locus_tag="ACHY3M_001618"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFS4316553.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3692..3790)
                     /locus_tag="ACHY3M_001619"
                     /pseudo
     CDS             complement(3692..3790)
                     /locus_tag="ACHY3M_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3917..4345
                     /locus_tag="ACHY3M_001620"
                     /pseudo
     CDS             3917..4345
                     /locus_tag="ACHY3M_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4450..5517
                     /locus_tag="ACHY3M_001621"
     CDS             4450..5517
                     /locus_tag="ACHY3M_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316554.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5764..6426)
                     /gene="ytfE"
                     /locus_tag="ACHY3M_001622"
     CDS             complement(5764..6426)
                     /gene="ytfE"
                     /locus_tag="ACHY3M_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFS4316555.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6534..7499)
                     /gene="ytfF"
                     /locus_tag="ACHY3M_001623"
     CDS             complement(6534..7499)
                     /gene="ytfF"
                     /locus_tag="ACHY3M_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4316556.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7607..8467)
                     /gene="qorB"
                     /locus_tag="ACHY3M_001624"
     CDS             complement(7607..8467)
                     /gene="qorB"
                     /locus_tag="ACHY3M_001624"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS4316557.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLANEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8556..8936
                     /gene="ytfH"
                     /locus_tag="ACHY3M_001625"
     CDS             8556..8936
                     /gene="ytfH"
                     /locus_tag="ACHY3M_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4316558.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(9065..11008)
                     /gene="cpdB"
                     /locus_tag="ACHY3M_001626"
     CDS             complement(9065..11008)
                     /gene="cpdB"
                     /locus_tag="ACHY3M_001626"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFS4316559.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAAYLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11198..11938
                     /gene="cysQ"
                     /locus_tag="ACHY3M_001627"
     CDS             11198..11938
                     /gene="cysQ"
                     /locus_tag="ACHY3M_001627"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFS4316560.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11928..12485)
                     /gene="ytfJ"
                     /locus_tag="ACHY3M_001628"
     CDS             complement(11928..12485)
                     /gene="ytfJ"
                     /locus_tag="ACHY3M_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS4316561.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDRALTQEEVQQVMDLLHKLINK"
     gene            12810..13016
                     /gene="ytfK"
                     /locus_tag="ACHY3M_001629"
     CDS             12810..13016
                     /gene="ytfK"
                     /locus_tag="ACHY3M_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFS4316562.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(13078..14421)
                     /gene="paeA"
                     /locus_tag="ACHY3M_001630"
     CDS             complement(13078..14421)
                     /gene="paeA"
                     /locus_tag="ACHY3M_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4316563.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14744..15382)
                     /gene="msrA"
                     /locus_tag="ACHY3M_001631"
     CDS             complement(14744..15382)
                     /gene="msrA"
                     /locus_tag="ACHY3M_001631"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4316564.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15588..17321
                     /gene="tamA"
                     /locus_tag="ACHY3M_001632"
     CDS             15588..17321
                     /gene="tamA"
                     /locus_tag="ACHY3M_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFS4316565.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTRGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17318..21097
                     /gene="tamB"
                     /locus_tag="ACHY3M_001633"
     CDS             17318..21097
                     /gene="tamB"
                     /locus_tag="ACHY3M_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFS4316566.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            21100..21441
                     /gene="ytfP"
                     /locus_tag="ACHY3M_001634"
     CDS             21100..21441
                     /gene="ytfP"
                     /locus_tag="ACHY3M_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFS4316567.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21653..21904
                     /gene="chpS"
                     /locus_tag="ACHY3M_001635"
     CDS             21653..21904
                     /gene="chpS"
                     /locus_tag="ACHY3M_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFS4316568.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21898..22248
                     /gene="chpB"
                     /locus_tag="ACHY3M_001636"
     CDS             21898..22248
                     /gene="chpB"
                     /locus_tag="ACHY3M_001636"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFS4316569.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22328..22858)
                     /gene="ppa"
                     /locus_tag="ACHY3M_001637"
     CDS             complement(22328..22858)
                     /gene="ppa"
                     /locus_tag="ACHY3M_001637"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFS4316570.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23168..24124
                     /gene="ytfQ"
                     /locus_tag="ACHY3M_001638"
     CDS             23168..24124
                     /gene="ytfQ"
                     /locus_tag="ACHY3M_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFS4316571.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24264..25766
                     /gene="ytfR"
                     /locus_tag="ACHY3M_001639"
     CDS             24264..25766
                     /gene="ytfR"
                     /locus_tag="ACHY3M_001639"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFS4316572.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGPALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25780..26802
                     /gene="ytfT"
                     /locus_tag="ACHY3M_001640"
     CDS             25780..26802
                     /gene="ytfT"
                     /locus_tag="ACHY3M_001640"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFS4316573.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26789..27784
                     /gene="yjfF"
                     /locus_tag="ACHY3M_001641"
     CDS             26789..27784
                     /gene="yjfF"
                     /locus_tag="ACHY3M_001641"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFS4316574.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27817..28815)
                     /gene="fbp"
                     /locus_tag="ACHY3M_001642"
     CDS             complement(27817..28815)
                     /gene="fbp"
                     /locus_tag="ACHY3M_001642"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFS4316575.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28991..30364
                     /gene="mpl"
                     /locus_tag="ACHY3M_001643"
     CDS             28991..30364
                     /gene="mpl"
                     /locus_tag="ACHY3M_001643"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFS4316576.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30520..31071)
                     /gene="yjgA"
                     /locus_tag="ACHY3M_001644"
     CDS             complement(30520..31071)
                     /gene="yjgA"
                     /locus_tag="ACHY3M_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFS4316577.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31165..32517
                     /gene="pmbA"
                     /locus_tag="ACHY3M_001645"
     CDS             31165..32517
                     /gene="pmbA"
                     /locus_tag="ACHY3M_001645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFS4316578.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32700..33086
                     /gene="cybC"
                     /locus_tag="ACHY3M_001646"
     CDS             32700..33086
                     /gene="cybC"
                     /locus_tag="ACHY3M_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFS4316579.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(33131..33595)
                     /gene="nrdG"
                     /locus_tag="ACHY3M_001647"
     CDS             complement(33131..33595)
                     /gene="nrdG"
                     /locus_tag="ACHY3M_001647"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFS4316580.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(33754..35892)
                     /gene="nrdD"
                     /locus_tag="ACHY3M_001648"
     CDS             complement(33754..35892)
                     /gene="nrdD"
                     /locus_tag="ACHY3M_001648"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4316581.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(36286..37941)
                     /gene="treC"
                     /locus_tag="ACHY3M_001649"
     CDS             complement(36286..37941)
                     /gene="treC"
                     /locus_tag="ACHY3M_001649"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4316582.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(37991..39412)
                     /gene="treB"
                     /locus_tag="ACHY3M_001650"
     CDS             complement(37991..39412)
                     /gene="treB"
                     /locus_tag="ACHY3M_001650"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFS4316583.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(39531..40478)
                     /gene="treR"
                     /locus_tag="ACHY3M_001651"
     CDS             complement(39531..40478)
                     /gene="treR"
                     /locus_tag="ACHY3M_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFS4316584.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            40663..40716
                     /gene="mgtL"
                     /locus_tag="ACHY3M_001652"
     CDS             40663..40716
                     /gene="mgtL"
                     /locus_tag="ACHY3M_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFS4316585.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            40857..43553
                     /gene="mgtA"
                     /locus_tag="ACHY3M_001653"
     CDS             40857..43553
                     /gene="mgtA"
                     /locus_tag="ACHY3M_001653"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4316586.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(43759..44145)
                     /gene="ridA"
                     /locus_tag="ACHY3M_001654"
     CDS             complement(43759..44145)
                     /gene="ridA"
                     /locus_tag="ACHY3M_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFS4316587.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(44218..44679)
                     /gene="pyrI"
                     /locus_tag="ACHY3M_001655"
     CDS             complement(44218..44679)
                     /gene="pyrI"
                     /locus_tag="ACHY3M_001655"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFS4316588.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(44692..45627)
                     /gene="pyrB"
                     /locus_tag="ACHY3M_001656"
     CDS             complement(44692..45627)
                     /gene="pyrB"
                     /locus_tag="ACHY3M_001656"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFS4316589.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(45631..45765)
                     /gene="pyrL"
                     /locus_tag="ACHY3M_001657"
     CDS             complement(45631..45765)
                     /gene="pyrL"
                     /locus_tag="ACHY3M_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFS4316590.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(46046..46441)
                     /gene="yjgH"
                     /locus_tag="ACHY3M_001658"
     CDS             complement(46046..46441)
                     /gene="yjgH"
                     /locus_tag="ACHY3M_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS4316591.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(46572..47285)
                     /gene="bdcA"
                     /locus_tag="ACHY3M_001659"
     CDS             complement(46572..47285)
                     /gene="bdcA"
                     /locus_tag="ACHY3M_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4316592.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            47356..47949
                     /gene="bdcR"
                     /locus_tag="ACHY3M_001660"
     CDS             47356..47949
                     /gene="bdcR"
                     /locus_tag="ACHY3M_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4316593.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            48094..48546
                     /gene="tabA"
                     /locus_tag="ACHY3M_001661"
     CDS             48094..48546
                     /gene="tabA"
                     /locus_tag="ACHY3M_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFS4316594.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            48669..>49472
                     /locus_tag="ACHY3M_001662"
     CDS             48669..>49472
                     /locus_tag="ACHY3M_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4316595.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIRDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLL"
BASE COUNT        11824 a        12304 c        13267 g        12077 t
ORIGIN      
        1 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagtacaagc
       61 agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtacacaagc cagcgttgcg
      121 cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca aagtaaattc agatgccagg
      181 tatgtggata tacagcgaac gccgatgtaa acggcgctcg taacatttta gcggcggggc
      241 acgccgttct tgcctgtgga gagatggtgc agtcaggccg ctcgttgaag caggaaccca
      301 ccgaaatgat tcaggcgaca gcctgaacga agcagggatc cacgtccttc agggcgtgga
      361 ggatgtcaac atatcagtat ggtttccccc caggcactgg aagccagacg taaggtcatc
      421 aaagagacaa tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac
      481 tgccatccgg ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc
      541 ccgtcaccac gccgctggcg ttctggcgga ttttcaccat ttgcccgttt tgcaaattac
      601 tcagcggctt acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc
      661 cgtggtcgcg gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt
      721 gattatcaat tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgctctt
      781 ctggctggcc ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt
      841 gtggggtgac taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa
      901 tatctaactg cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca
      961 gaaatccgac caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca
     1021 gcgggtccat gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta
     1081 gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct
     1141 atgaataagc taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga
     1201 tattgctatt gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc
     1261 aaagaagtgt ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg
     1321 accaccccaa cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta
     1381 gggcaacaac tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt
     1441 attgccgatg cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgcg
     1501 ctgcgtgaaa tccacgagcg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct
     1561 gctgaaggtg tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa
     1621 tctggcctgc aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac
     1681 cgcgttcgtg ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt
     1741 gctcgtggtg aacccgctga atttccggtt aatggcgtga tccctggctg gattgaagca
     1801 ctgactctga tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat
     1861 ggcgagcgcg gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa
     1921 ctgctggaaa tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat
     1981 tcagcaataa acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa
     2041 gcgtgtattt ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat
     2101 aaaattaaca ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat
     2161 gagtcccgat acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac
     2221 acagacaggt acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc
     2281 aggctccggc tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg
     2341 ccattggcgg tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg
     2401 ccgggccgtc gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc
     2461 gggcaatggg ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt
     2521 ccgatttact cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg
     2581 ttgtaaccgg tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg
     2641 atctctccga ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg
     2701 ccaccgtgaa aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca
     2761 ttgtgtcgct gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg
     2821 gtgtggaagc gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa
     2881 gtggcttctt tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag
     2941 gtacaaccgc tgcggaaacc aaagatccgg agaaatcact gccgcgtgca attaactcta
     3001 ttccgatccg tatcattatg ttctacgtct tcgcgctgat tgtaattatg tccgtgacgc
     3061 cgtggagttc ggtagtcccg gagaaaagcc cgtttgttga actgttcgtg ttggtgggtt
     3121 tgcctgcggc tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta
     3181 acagcggcgt cttctctacc agccgtatgc tgtttggcct ggcccaggaa ggtgttgcac
     3241 cgaaagcgtt cgctaaactc tctaagcgcg cagtacccgc gaaagggctg accttctcct
     3301 gcatctgtct gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt
     3361 tcacgatgat cacgacggtt tccgcgattc tgtttatgtt cgtctggacg attatccttt
     3421 gctcgtacct ggtgtatcgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga
     3481 tgccgctcgg caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtgatcgtgc
     3541 tgctgacact ggaagatgac actcgccagg cgctgctggt taccccgctg tggtttatcg
     3601 cgctggggtt gggctggctg tttattggta aaaaacgcat ggcgaagtaa tgccgctaag
     3661 gcacctgcgg gtgtctttca ggctgcaaaa ttgcctgatg cgctacgctt atcaggccta
     3721 cagaacctct gcaatatatt gagtttgcgt gcttttgtag gccgggtaag gcgtttacgc
     3781 cgcatccggc atgaacaaag cacactttgt cagcaatctc ctttgtcttt atacattcct
     3841 aaaaatactt cccgtcatcg acagctctca ggcgcgggct taccgtggat ctttgccact
     3901 cattgcagag ttcactatgg ctaagcacag ttggttatta atcgccgcat tacctctctc
     3961 catttcacct tcctggggcg cagattttta ttaccgccag caggagaaag gcacggttta
     4021 tgttgccgaa cagaagggag aaaaggatga gatcctctcc gaactgccgg gcgttaattt
     4081 ttcccgcctc tggcgtattg ccaatttagc caataaccaa gaaacccgct tactgtccga
     4141 ttttaatccc gataagttcg attgcgatga tcggaataat tgccagcata cctggctcac
     4201 cgatggacgc tctgttcttt ggtctggcaa agtcctgaaa aatccgcccg gtgaacctaa
     4261 tgtcgatgcc gccagttttc aggcattcgg tgctttcgct gccgataaac gcagcgtcta
     4321 ttttgacggt cagcgtaccg atgatagatg aagtggatat gtatatagct gtgtctcagt
     4381 tgcaaacaaa tatttatgta ccgaaataaa agtatcaata gacaattaat taataaatca
     4441 ggagataaag tggctatatt acatccacag gagtgctggt tgctggaaag aataatgtcg
     4501 ccggaatatt atcggcgtcg ttttgagggc tggcaggagt ttgtcgagct gtgcgagcga
     4561 caggtggcag agtggagcaa gaccatgccg ctggatgtgc gccgtcgtcc gctgtgcgag
     4621 cagattgacg tcgtctgggg cgggcgagtg ttgcccaata tccgcagtac tttaaagtcg
     4681 gtacagtatg actttattca gctacagcag ggggacctgc gggtactgca atccgggggc
     4741 aatattagca gtgatatgaa aggactaatt gattatccgt cggactggat gtcactcgcc
     4801 gcgcaaaaac agtatgacag actgaaatgg cgaggtgcac attataataa tttaatcaga
     4861 cgtacctcgg gcggatactg gtatgacggc gaactgactt attattatga ggaaagtctg
     4921 cacggcccgc tggccctgcc gatgcaatta ccgttgtatg agctggatag tagcgtatac
     4981 ctgcgtgagg atgactcggt aacggtggct ggcctgtatc tgccggatat cccagatgcc
     5041 agcgcccaac tgttgtaccg gagtgaacat attcccgagg cttggcaggg gcgggtacga
     5101 acgaagtatg tcaacgaggc tgggatacaa gaatattact gggaaagcgg ggcgtgggca
     5161 aaatgcaatt ggaaacgtat acggcgggtg gcaaaccgat ttatcaatgt gccgccggag
     5221 ggatttttcc cacagggcat gccggaggag ttgtacaact ggccacagcg cgaagcgcag
     5281 tatgttacag accggcagcg gatagcggcc tttagcggtg aagcttgtcc tcatagcggg
     5341 gagtggtcga tttttgtaga aggtagacaa gcgacggtga cactggagca gggagagcag
     5401 atgccagaat ggacggacag gaaaatggag ggggagtata aaagaggcga gaaattccat
     5461 gtgctgtggt cgctgatgaa ccgccatgat ggcgggtcgg tgtgggtgga agcgtagcgg
     5521 tagctggatg tacacgtaaa aaccagggaa tgggtgattt tattctggct tatacaggag
     5581 cgatgaataa gtaattatat taatgaaaaa aataagttag taaccgttga aggaacttat
     5641 tattggctga ataatgaatt ttcccagttc agtagataca aaagattgtc ccggagtggt
     5701 gaataactca aagcatttga aaacgcacct gattgtgtaa accaggtgcg ttgatcgaac
     5761 aaattactca cccgccagcg cgcgtgggaa cagtacattg ttttccaggc tgatgtgatc
     5821 catcaggtca tcaatcagtt cattaatacc gttatacatc gctttccagg tggtgcaggc
     5881 ttccggcggc ggtgtgacgt tattggtggt gtgtttaatc acttccagca gttcgcccgc
     5941 ttcatcgtgc tcgctttcca tcacgctgat tggtcccatt gcctggctgc ccatgccttg
     6001 tttgatcatc gggaagagga tctgctcttc tttcatcatg tggctggaaa gctcttcatg
     6061 cagcatagtc aggtattttg tcagcccttt tggcacgctc ggtttgtcgg cgtgaacgcg
     6121 ctcgacttta gtcgcttgca ggatcagctc cggcagctgc tcgcggtgac gatcgtggta
     6181 gcgcacgatg atatggtcaa tgatttctgc cagcggggcg ctacgccagt ctttctcaat
     6241 cggttgttca gcaagctttg ccagttcagc ttcaatgacc tcaacatcca gttctttacg
     6301 tgccgccgcg cgcgccagcg tctgcttacc gccacagcag taatccatat catatttacg
     6361 aaacagagcc gaagcgcgag ggatagagag cgccagttca cctaaaggtt ggtcgcgata
     6421 agccatagct gatacctcat tcttaataag ataagatgta ttttaaatgc atctttaagg
     6481 caaaagctat aacccttacg tagcagaggc ttatttaact cactgccagc gtgtcaactt
     6541 tctgagacag attcagttaa aggtttttct ggttttgctc tgaccgcaat gaccacgcca
     6601 atcactaaca gcgcgatacc gctcagcgtc attagcggcg gcatttgctg gcggagtaaa
     6661 aaggtgtaca gcaaacctgc cagcgtttcg aaaacaatca gcggcccgag aatcactgtc
     6721 ggtaatcgct ggctggcgac gttccagcag agtgcgccaa cccatgagca aagcacggct
     6781 atcgcaacca tcagactaat aaacaccagc ggacgggggc caaaaggtaa ggagaagtcc
     6841 ggcgtttgta tattcagcca gtaacaggcg acgagatagc cgatgagaga aaccggcagc
     6901 gtgaccagcg cctgcgccgt cgcccacatc atcggatgtt tgtcgggatt ttcccgcagc
     6961 cagcgggcgt tgcgcagggc ataccatgcc cagcagacca cggaaactaa cgctagcacg
     7021 atgccagagg tataacgtgc ccagtcaaaa tcggggagtc cgtggtttaa ctcagcaata
     7081 ttcacactcg ccaggccgat gccaatacaa atcagtgccg gggcgagttt tccccacgcg
     7141 agtttgccgt cgcgctggct ataaagcaga ttggcaaaga caggaatgac caccggcagg
     7201 gtgccgataa tcatcgtgga aacaggcgcg ccagtacgtt gaatggcact ggcaaggcag
     7261 aagtaataaa tgaggttgcc catcatagtg agcatcaagg cggtaagcca gtcccgacgc
     7321 gccaactgac gcagacgcac gcgtcccagc caggcaatgg gcagcgcaat taaccctaac
     7381 gccagataac gccccatcga ctgcaacatc gccgggtatt ccggtacgat caacggcccg
     7441 acaaaaataa gcccccacat caaccctgct aacagggcgt acagcacgcc gctaatcatt
     7501 actggcatcc attggtctgt cagaagaaag ttcaggaagc agacatgatt gctcccggca
     7561 tcccgtcatt attagagagg atgcgggagg atgccacttt aattaactag ttattaacat
     7621 taaaaagatg gcttacgctt tcggctaacg ttgtcgttgg gcggccaatc aatttgctaa
     7681 gcgttttgct gttatcaaac agaccacctt tcgatgcgcc aacgtcagaa tccgccagca
     7741 tatccgccag tccgtcgggc agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc
     7801 tcagattttg ataggtaacc tgtttgccgc tctgtttggt cagctctgcc gctaactgtg
     7861 tcaatgtcca ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc
     7921 taatcacgcg tgccgcagct gccgcataat ctgcccgcgt tgctgaggcg attttgccat
     7981 cgcccgccgc accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt
     8041 tttcgctgta ccagccgttg cgcagcaggg tgtaaacgat gccagaatca gccaacattt
     8101 tctccgtctc gatgtgctca ttggcgaggc cgagcgggga ggtatctgca tgtagcaggc
     8161 tggtataagc gataaatttc acaccagccg cctttgcggc attaataaca ttacgatgct
     8221 gcggggcacg ttgacccact tcgctggaag agatcagcag tagtttttcc actccctgaa
     8281 gtgcagatgt cagtgcggct tcatcgccgt agtcagcctg acgcacggta atgccttgtg
     8341 ctgccagagc ctgggctttt gccggattac gaacgatagc cactatttgg ctgacaggaa
     8401 ccgttttctt caaggattca ataacatagt gaccaagttg gccagtggca ccagtaatag
     8461 cgatcatggg aagtctccat cgttttgtct tgtgtttaaa tacgctaaca cctaaactta
     8521 cttttagtaa gtacgcacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac
     8581 aactgaaaga gggtaatctc tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg
     8641 tcaccagccg ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta
     8701 gcgacctgcg gcgcaaaatc ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg
     8761 cgttagaaca ggatggtttt cttaaccgta tcgcgtatcc ggtggtgccg ccgcatgtgg
     8821 aatatagcct cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact
     8881 ggattgagtt gaatttgccc gaggtgttgg cggtgcggga tgaacgtgcg gcataactgc
     8941 cggatgcgct gagcttatct ggtctacggt gcatggcttg tagggcggat aagatgcgcc
     9001 agcatcgcat ccgccaataa atgccggatg cggttgatga tttataggcc gaaaaagaag
     9061 tgttttactt actcaaatcc acctgataaa tcgcaaaccc gatatcatcg gtcgcgactt
     9121 tattcatcgg atattgccct ttctctttaa taaacgctgc ggctttatct gacggagaag
     9181 tttcgaaacg gatatccagt ttcttatcgc cagctatcgg tgctaaacgc cagttgttat
     9241 ctgccgccgg gtgaatttcc cccgcacgtt tcgactcatc agcaatccac gctgccagca
     9301 ccgagcggtt ctcatccggt gaagcaaaag cgatatggct gtcgcccgta ccggcaaatt
     9361 tgccgccgta agcgcgatag ttattggtgg caacgaggaa catggcgttc ggatcaatcg
     9421 gcttgccatt aaaggtcagg ttcttaatcc tttccgcatt ggcattaatc atctggcact
     9481 cgccgtcata acgggcaggt tgggtaacat caatctgata attcacacca tcaatcacat
     9541 caaagttata ggtgcggaaa ccatcccagt tgatgagtga ttgcggtttc gtgctgttag
     9601 gatcaatctg gttaaactgt ccggcggagc actccagcca ctctttcacc tctttaccgc
     9661 tggctttcac cacaatcagc gtattggggt agagataaag ataggcggca ttacggaagg
     9721 tcaactggcc tttttccacc tccacatagc ttgccgggtc atttttgcga ccaccaactt
     9781 taaacggtgc ggcagctgaa agcaccggca gttttgccag atccggatcg ccctgaatgt
     9841 aatgttcgac atacgctttt tgcgcgttgt tcactacttg cacggtcgga tcgtcctgca
     9901 ccagcgccag atagctatac atattgtcgg cagatttacc gattggcttg ctgacgaact
     9961 ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag cttgctgtct tccgccgcga
    10021 gggatttttt attggcgatg tcgtaaatcg gccgagcttc cgctttcgcc tgcgtcacct
    10081 gccatttacc gctgtcatta ctgagttgta agtcgaccac cccaagatga tcgccccaca
    10141 tgcccggcat taccgccgga acaccattca gcgtgccttt ggtgatatca gccccttcga
    10201 tatcagcaaa atctttaccc gggaaaacgg cgtgagcatg accaaacata atggcgttaa
    10261 cgcccggaat ttcactgagg taataaactg agttttccgc cattacttta tacggatcgg
    10321 cagacagccc ggaatgcgcc agaacgacaa cgacatcggc acctttctcg cgcatttcag
    10381 gcacgtattt gcgcacggtt tcggtaatat cattcaccgt cacttttccg gataaattag
    10441 ctttatccca gcccatgatt tgcggcggca cgacgccaat atagccaatc ttcagcgtct
    10501 gtttttttcc gtctttatcg accacttcgg tatctttaat taaatacggt gtaaacattg
    10561 gctgtttggt tctggcgtca atgacgttgg catttacata agggaatttc gctcccgcca
    10621 gcgcattttt caggtaatcc agaccgtagt taaattcatg attgccgagt gtaccgaccg
    10681 tataatccag cgtattcagc gccttataaa ccggatgaac atcacctgct tttaatcctt
    10741 tcgccgacat gtaatcggcc agcggactcc cctgaatcaa atcgccgtta tcgaccagta
    10801 cgctgttttt cacttcattg cgggcatcgt taatcaggct tgccgtacgt accagtccga
    10861 atttttccgt ggcggtgtct ttgtaataat cgaaatccat catgttgcta tgcagatcag
    10921 tggtttccat gatacgcaga tcgaccgtcg ctgcattcac actggcggca atcagcgtgg
    10981 ccaggagcgt tgcgctaaac ttaatcatca gggacatcct tttatcatcg ggaatacgaa
    11041 agaaaaggaa gaataaacgt cttacttata gaacagtgaa gaatgccaca attttacgct
    11101 ttgaaaatga tgacactatc acagttggcg cattcattaa cgatagggta taagtaaaac
    11161 aataagttaa caccgctcac agagacgagg tggagaaatg ttagatcaag tatgccagct
    11221 tgcacggaat gcaggcgatg ccattatgca ggtctacgac gggacgaaac cgatggacgt
    11281 cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat attgccgctc acaccgttat
    11341 catggacggt ttacgtacgc tgacaccgga tattccggtc ctttctgaag aagatcctcc
    11401 cggttgggaa gtccgtcagc actggcagcg ttactggctg gtagacccgc tggatggtac
    11461 taaagagttt attaaacgca atggcgaatt caccgttaac attgcgctca ttgaccatgg
    11521 caaaccgatt ttaggtgtgg tgtatgcgcc ggtaatgaac gtaatgtaca gcgcggcaga
    11581 aggcaaggcc tggaaagaag agtgcggtgt gcgcaagcag attcaggtcc gcgatgcgcg
    11641 cccgccgctg gtggttatca gccgttccca tgcagatgcg gagctgaaag agtatctgca
    11701 acagcttggc gaacatcaga ccacgtccat cggctcttcg ctgaaattct gcctggtggc
    11761 ggaaggacag gcgcagctgt acccgcgctt cggaccaacg aatatttggg acaccgccgc
    11821 tggacatgct gtggccgcag ctgccggagc gcacgttcac gactggcagg gtaaaccgct
    11881 ggattacact ccgcgcgagt cgttcctgaa tcctgggttc agagtgtcta tttattaatt
    11941 aatttatgca gcaggtccat cacttgctgc acctcttcct gagtaagcgc cctgtctttg
    12001 gcccattgca cgcgaccgtc tttatccagc accactacag cggaactttt ttcatccagc
    12061 tgccaggcac cgcgtgcgac gccattgcta tcaacaataa actgcgacca gggataaagc
    12121 tttttattac tctccagact gctgcgcaca aacatgccgg aacccggaat tgcgtcgtcg
    12181 gtgttaacaa tggtggtggt ctggtaacga tcgtgcggta acttcgctga tttaatcgct
    12241 tcaatcagcg tcgcgttttt ctcttttgca gaggtgcgac cagcaatatg ttgcagtact
    12301 cgcacttttc ccactaactg cgcgctgttc caggttttgt agctaaactg atctttatca
    12361 agcaccaact cgccacgatc ggtgatgcca atcggcggca ctcgctgacc ggtttcgaac
    12421 tgatgtgcgg aagccatcat cggcaacagc aggcaggtga gtgccagaat cttgcgtagg
    12481 gtcatggtgt ttccttctta tgatatgcag gtgatccgac cacttgggtc tgagttttaa
    12541 tcatatgtgc tatttatcga atttcccgca agtgtgatgc cagtttgcgg tcaagcgcac
    12601 aaatcatatg aaaaatgaat gcttatactg aagaccgcgc ttcggtaaaa agataattct
    12661 gaataattgt aacctttagg taaaaaaagt tatacgcggt ggaaacattg cccggatagt
    12721 ctatagtcac taggcattaa aatttgcgcc tcataatagt tgggccgatt gtggcaccgc
    12781 acaggcgtaa tactcagcag gagataacaa tgaaaatttt ccaacggtac aacccacttc
    12841 aggtggcgaa gtacgtaaag atcctgttcc gtggacggtt atacatcaag gacgttggcg
    12901 cttttgaatt tgataagggt aagattctta tcccaaaagt gaaggataag ctgcatttgt
    12961 cagtgatgtc cgaagttaac cgtcaggtta tgcgtctgca aacagagatg gcttaaccaa
    13021 agtgctatgc agtaataaga cggctcctga ttcaggagcc gttgatgttt ctgggggtta
    13081 cgcgacgctt tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg cggtggcctt
    13141 gctgtcgatc cgggtcacca gcagctggtc gatgcggtag ttatcgatat ccaccacttc
    13201 aaatttgtag ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac gcagcataaa
    13261 catcataaag cctccgatgg tttcgtagtt gcccgactgc gggaactcgt caatatccag
    13321 cacgcgcatg acgtcgtcaa ttggggtgcc gccgtcaatc agccatgagt tctcatcacg
    13381 ggcgacaatc tgctcttcca gcccctgacc gaccagatcg cccatcagcg tggtcatcac
    13441 gtcgttgagg gtgatgatcc ccaccaccag cgcgtactcg ttcatgatca ccgcgaagtc
    13501 ttcacctgcg gttttaaaac tttccaacgc ctctgaaagg gttaacgtat ccggcacaat
    13561 cagcgtgttg cgaatttgta cgccgctgtt cagtgccagg ctttggttag ccagcacgcg
    13621 gttcagcagg tctttagaat cgacataacc gatgatgtgg tcaatatctt cattacagac
    13681 gaggaactta gagtgcggat gttccgccac cttattcttc aggctttgct catcttcgtg
    13741 gagatcaaac caaatcacgt tttcacgcgg tgtcattgaa gacggaacgg tacgggattc
    13801 cagctcaaag acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc ccgccagcgc
    13861 accggcttcc actaccgcgt agatgtcatc agaagtgatg tcatctttac gtaccattgg
    13921 cagtttgaaa atacggaaga tcatgttcgc caggccgttg aagaaccaca ccagcggggt
    13981 gcaaacgtac aggcagaagc gcatcgggtt gatgatacgc aaagccaccg cttctggcgc
    14041 aatcataccg atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc cagtcactaa
    14101 cgagaaagag agaataaagc tcagttgctc agagagttct gccgacatat agcgggagaa
    14161 caggctgtga aaagctggag aaaatgccgc atcaccgacg ataccgccga gaatcgcaac
    14221 tgcgttcaga ccgatttgga ccaccgtaaa gaacatgccg ggattttcct gcatattcag
    14281 aacgcgttgg gcatttatat tgccttcatc agccagcagt ttaagtttga ttttgcgtga
    14341 ggcggcaagt gagatctcag acatcgagaa gaacgcactt acagcgatca agcagagtat
    14401 gactaaaata ctgtttaaca tatcttatcc gacctttcag gccagatcct cggaagggga
    14461 agttgattaa tttgtgtgaa atacacattg aaagccgatt cgaagaaagt gatccggcat
    14521 tttcagtggc tagtatagcg taaggtactg taaagtcgcc agagggttaa aattcagact
    14581 gctgataaat gcaatactgc ctgatgcgct atgccgatca ggcctacgca gtccctgcaa
    14641 tatattgaat ttggacgatt ttgtaggcct ggtaaggcat tcacgctgca ttcggcataa
    14701 acaaaacgca ctttatcaac aatctacatt tgtacccgta acgctatgct tccggtggca
    14761 gacagacgcc aattccgcca attccacagt aaccatacgg gtttttatgc agatattgct
    14821 ggtggtcatc ttcggcataa taaaacggtg tggcgttagc gatttccgtg gtgatgtgac
    14881 gatcgtcatc ggcggcaagc atcgccgcct gaaaacgttc cagactggcg cgagctgcgg
    14941 catcctgttc tggggtcagc ggataaatcg ctgaacgata ctgcgtgccg tggtcattgc
    15001 cctgacgcat tccctgggcg ggatcgtgat tctcccaaaa tacctgtagc aactgctcat
    15061 agctgatgac agaaggatcg taaacaatgc gtaccgcttc ggcatgaccc gtatcaccgg
    15121 agcacacttc ccgataagtc ggatttggcg tatagccgcc ggtatagcct gcggcggtgc
    15181 tgtaaacgcc gggtaactgc cagaacagac gctccacacc ccagaaacaa cccatcgcaa
    15241 aaatggcaat ctccattccg tcaggtacat tggtcattga gtgaccgttg accgcatgca
    15301 gcgtggctac gggcatcggg gtgttacgtc caggcagggc atcggcgggg gaaaccagat
    15361 gctttttatc aaataaactc atggtgtcgc tctcccgaaa atcggtcatt ggggttaagg
    15421 ttgtaacaag aggcgtattt gcacacaata accaccgtga ataggtctaa agtaaaacat
    15481 aagaaatatt tggggtttag tctgcttttt aatccatatt actggatttt tgttaagccg
    15541 tttaacggcg ttccaggggc aggaaaaaag gatattcagg agaaaatgtg cgctatatcc
    15601 gacagttatg ctgtgtaagc ttactctgct taagcggatc tgccgtcgcc gcgaacgtcc
    15661 gtctacaggt cgaggggtta tcgggacagc tggaaaagaa cgttcgtgcg cagctttcta
    15721 cgattgaaag tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc gatgatgcca
    15781 tccgcgaagg tctgaaagcg ctgggttatt accagccgac cattgaattt gatctccgtc
    15841 caccgccaaa gaaagggcgg caggtattga tcgccaaagt cacgccaggc gtgccggtgt
    15901 taattggcgg caccgatgtg gtattgcgcg gcggcgcgcg gaccgataaa gactatttga
    15961 aattgctcga tactcgcccg gctattggca cggtactgaa ccagggcgat tatgaaaatt
    16021 tcaaaaagtc cttaaccagc attgcgttgc gtaaaggtta tttcgatagc gaatttacca
    16081 aagcgcagct gggcattgcg ctcggcctgc ataaagcctt ctgggatatt gattataaca
    16141 gtggcgaacg ttaccgcttt gggcatgtga cctttgaagg atcacaaatc cgcgatgaat
    16201 acctgcaaaa tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa gatctggcgg
    16261 aacttaaccg tcgactttct gctaccggct ggtttaactc ggtcgtagtg gctccacaat
    16321 ttgataaagc gcgcgaaacg aaagtattac ccttgacggg cgtggtttcg ccgcgaactg
    16381 aaaacaccat cgaaaccggg gtcggttact ctacggacgt gggaccgcgc gtgaaagcta
    16441 cgtggaaaaa gccgtggatg aactcttatg gtcacagtct gaccaccagt accagtattt
    16501 ccgcgccgga acagaccctc gacttcagct ataaaatgcc gctgctgaag aatccactgg
    16561 aacaatatta tttggtgcag ggcggtttta agcgcactga cctgaacgat accgaatctg
    16621 actccactac gctggtggct tctcgctact gggatctctc cagcggctgg cagcgtgcca
    16681 ttaacctgcg ctggagtctc gaccacttta ctcagggtga aattaccaac accacgatgc
    16741 tgttttatcc tggggtgatg attagccgca cgcgttctcg tggtggcctg atgccaacca
    16801 ggggcgactc gcaacgctac tctatcgact actccaacac ggcctggggc tcagatgtcg
    16861 atttctccgt tttccaggcg cagaacgtct ggatccgcac actgtacgat cgccatcgtt
    16921 tcgttacacg cggcacgctg ggctggattg aaaccggtga tttcgacaaa gtaccgccgg
    16981 atctgcgttt tttcgccggg ggcgatcgca gtattcgcgg ctacaaatac aaatctatcg
    17041 ccccgaaata cgccaacggt gacctgaaag gggcctcgaa gttgataacc ggatcgctgg
    17101 aataccagta caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat agtggcgaag
    17161 cggtaagcga tattcgccgc agcgacttta aaaccggtac cggggtcggc gtgcgctggg
    17221 aatcgccggt cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat aaagacgaac
    17281 acgggttaca gttttacatc ggtctggggc cagaattatg agtttatgga aaaaaatcag
    17341 cctcggcgtg gttatcgtta tcttactgtt gctgggatcg gtggcgtttc tggtgggcac
    17401 caccagcggc ctgcatcttg tatttaaagc ggcggatcgc tgggtgccag gactggatat
    17461 tggcaaggtc accggcggct ggcgcgatct caccttgtct gacgttcgtt atgagcagcc
    17521 aggcgtggcg gtaaaagcgg gtaatctgca tctggcggtc ggtcttgagt gcctgtggaa
    17581 cagtagcgtt tgtattaatg acctggcgct gaaagacatt caggtcaaca tcgacagtaa
    17641 aaaaatgcct ccttctgaac aggttgaaga agaggaagat agcggtccgc tggatctctc
    17701 cacgccgtat cccatcaccc tgacacgggt ggcgctggac aacgtcaaca tcaagattga
    17761 tgacaccacg gtgtcggtga tggacttcac ctccggcctg aactggcagg agaaaaccct
    17821 gaccctgaaa ccgacgtcgc tgaaaggcct gctgattgct ctgccgaaag tggcggaagt
    17881 ggcgcaggaa gaagtggtcg aaccgaaaat tgaaaatccg cagccggagg aaaagccgct
    17941 cggcgaaacg ctgaaagatc tcttttctcg cccggtattg ccggaaatga ccgacttgca
    18001 tttgccgctt aacctgaaca ttgaagagtt taaaggcgag cagttacgcg tgacgggcga
    18061 cacggacatc accgtgcgca ccatgctgct gaaagtgagc agcattgacg gcaatactaa
    18121 actggacgcc ctggatatcg attccagtca agggatcgtc aacgccagcg gcacggcgca
    18181 gctgtcagac aactggccgg tggatatcac cctcaacagc acactgaacg tggagccgtt
    18241 gaaaggtgaa aaagtgaagc taaaagtggg cggcgcgctg cgcgaacagc tggagattgg
    18301 cgttaatctt tccggtccgg tggatatgga tttacgcgcc cagacgcgac tggcggaagc
    18361 cggattgcca ctcaacgtgg aagtgaacag caaacagctt tactggccgt tcaccggtga
    18421 gaagcagtat caggcggatg atctgaaact gaaacttacc ggcaaaatga ccgattacac
    18481 gctctctatg cgtacggcag tgaagggact ggagatcccg ccagccacca ttacccttga
    18541 tgccaaaggt aatgaacagc aggtcaatct cgacaaactc accgtcgcgg cgctggaagg
    18601 gaaaactgaa ctcaaggcgt tgctcgactg gcagcaggcc attagttggc gcggtgagct
    18661 aacgcttaac ggcattaaca ccgccaaaga gatcccagag tggccgtcga aactcaatgg
    18721 cttgattaaa acccgcggta gcctgtacgg cggcacttgg cagatggagg tgccagaact
    18781 gaagctgacc ggtaacgtca aacagaacaa agtgaacgtt gacggcacgc tgaaaggcaa
    18841 cagttatatg cagtggatga tcccagggct tcatctggaa ctggggccaa acagtgccga
    18901 agtgaaaggc gagctggggg taaaagatct caatcttgat gccaccatca acgcgccggg
    18961 gctggataac gcgctgccgg ggcttggcgg tacagcgaaa gggctggtga aagttcgcgg
    19021 cacggtggaa gcgccacaac tactggcgga catcaccgcg cgcggcctgc gctggcagga
    19081 gctaaccgtg gcgcaggttc gcgtggaagg cgatatcaaa tccaccgatc agattgcggg
    19141 taaactcgac gtgcgcgttg agcaaatttc acagccggat gtaaatatca acctcgtcac
    19201 cctgaatgcc aaaggcagcg aaaaacagca cgagttacag ttgcggattc agggcgagcc
    19261 ggtttccggg cagcttaatc tggcaggaag ttttgatcgc aaagaagaac gctggaaggg
    19321 aactcttagc aatacccgct tccagacgcc ggtcggcccg tggtcgctga cccgcgatat
    19381 tgcgctggat taccgcaata aggagcaaaa aatcagcatc gggccacact gctggcttaa
    19441 cccgaatgcg gaactgtgcg tgccgcaaac tatcgatgcg ggggccgaag ggcgtgcggt
    19501 ggtgaatctc aaccgcttcg acctcgccat gctgaaaccg tttatgccag aaaccactca
    19561 ggccagcggt atcttcacgg gtaaagcgga tgttgcctgg gacaccacga aagaggggct
    19621 gccgcagggc agtatcaccc tttcggggcg taacgtgcag gtaacgcaaa ccgtcaacga
    19681 tgcggcgctg cccgtggcgt ttcagacact gaatctgacg gcggaattgc gtaacaaccg
    19741 tgccgaattg ggctggacca tccgcctgac caataacggc cagtttgatg gacaggtgca
    19801 ggtgaccgat ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc gtaacttcaa
    19861 ccttgcgatg ataaacccca tctttaaccg tggggaaaaa gcagcgggga tggtgagtgc
    19921 caacttgcgt ctgggtggtg atgtgcaaag cccgcagttg tttggtcagc ttcaggttac
    19981 gggtgtggat atcgacggca actttatgcc gtttgatatg caaccgagcc agcttgcggt
    20041 caactttaat ggtatgcgct cgacgcttgc cggtacagta cggacccagc agggtgaaat
    20101 ctacctgaac ggtgatgccg actggagcca aattgaaaac tggcgggcgc gggtaacggc
    20161 gaaaggcagt aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg tatcgccaga
    20221 tgttgtcttc gaggctacac caaacctgtt taccctcgat ggtcgcgtgg atgtcccgtg
    20281 ggcgcgcatc gtggtgcacg atctgccgga aagcgcagta ggcgtctcca gcgatgtggt
    20341 gatgcttaac gataacctgc aacctgaaga gccgaaaacg gcgtcgattc cgattaacag
    20401 taacctgatt gtccacgttg gcaacaatgt gcgcattgac gcctttggcc tgaaagcgcg
    20461 gctgacgggc gatcttaacg tcgttcagga caaacaaggg ctgggcctga acgggcagat
    20521 caacatccct gaagggcgct tccatgccta tggtcaggat ctgattgtgc gtaaaggtga
    20581 gttactgttc tctggtccgc cggatcaacc gtatcttaat attgaagcta ttcgtaaccc
    20641 ggatgctaca gaagacgacg taatcgccgg agttcgcgtc actggtctgg cggacgaacc
    20701 gaaagcggag atcttctctg acccggcgat gtcgcaacaa gctgcattgt cttatttgct
    20761 acgtggacaa gggctggaga gcgatcagag cgacagtgcg gcaatgacct cgatgctgat
    20821 tggtctgggg gttgcgcaaa gtggccagat tgtgggtaaa atcggcgaga cgtttggcgt
    20881 aagcaattta gcgctcgaca cccagggagt aggcgactcc tcccaggtag tggtcagcgg
    20941 ctatgtattg ccaggtctgc aagtgaaata tggcgtgggt atatttgact ctatagcaac
    21001 actcacgtta cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt ctggtgtaga
    21061 ccaggcactg gatttgctct atcagttcga gttttagcaa tgcgaatatt tgtctacggc
    21121 agtttacgcc acaaacaagg caacagtcac tggatgacca atgcccagtt actgggcgat
    21181 ttcagtatcg ataactacca gttgtatagc ctgggccact atccaggcgc agttccgggg
    21241 aacggaacag tacacggtga agtttatcgt attgacaacg ccacgctggc cgaacttgat
    21301 gccttgcgca ccaggggcgg tgaatacgcg cgccagttga ttcagacgcc gtacgggagt
    21361 gcatggatgt acgtttatca acgacccgtc gatggattaa agctaattga aagcggcgac
    21421 tggttagaca gggataagta accatatgca tacgccacct tcgggtggcg ttgttttttg
    21481 cgagacgact cgcattctgt tttgtaattc cctcaccctt tgcttttctc tccgagccgc
    21541 tttccatatc tgttaacgca taaaaaactc tgctggcgtt cacaaatgcg caggggtaaa
    21601 acgtttcctg tagcaccgtg agttatactt tgtataactt aaggaggtgc agatgcgtat
    21661 taccataaaa agatggggga acagtgcagg tatggtcatt cccaatatcg taatgaaaga
    21721 acttaactta cagccggggc agagcgtgga agcgcaggtg agcaacaacc aactgattct
    21781 gacacccatc tccaggcgct actcgcttga tgaactgctg gcacagtgtg acatgaacgc
    21841 cgcggaactt agcgagcagg atgtctgggg taaatccacc cctgcgggtg acgaaatatg
    21901 gtaaagaaaa gtgaatttga acggggagac attgtgctgg ttggctttga tccagcaagc
    21961 ggccatgaac agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca agcctttaat
    22021 caactgggaa tgacgctggt ggcccccatt acgcagggcg gaaattttgc ccgttatgcc
    22081 ggatttagcg ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt gctggtgaat
    22141 caggtgcgga tgatggatct acgcgcccgg ctggcaaagc gtattggtct ggctgcggat
    22201 gaggtggtgg aagaggcgtt attacgcttg caggcggtgg tggaataagg tgtgtttatt
    22261 tatcgcgggc ataaaaaaac ccttactaac cgaagctcgg cgttcagggt tattgcgcta
    22321 gaagaactta tttattcttt gcgcgctcga aggaagcaac gatttcagct ttagcggctt
    22381 ctgcgttttc ccaaccttca actttcaccc acttgccttt ttcgaggtct ttgtagtgct
    22441 cgaagaagtg agcgatctgc gctttcagca gctctggcag atcgttaacg tctttaatgt
    22501 gatcgtattc tttgctcagc ttgctgtgcg gaaccgcaac cagtttcgca tcttcaccgg
    22561 cttcgtcggt cattttcaga acgccaaccg gacggcaacg gatcacagaa cccggctgca
    22621 gcgggtacgg agtcgggacc agtacgtcaa ccgggtcacc gtccagagac agggtgtggt
    22681 tgatgtaacc gtagttgcac ggatagaaca tcgcggtgga catgaagcgg tcaacgaaca
    22741 gtgcgccgct ctctttgtcg atttcgtatt tgatcggatc tgcgttagcc gggatctcaa
    22801 taacaacgta gatgtcttcc ggcagatctt tacccgcagg gacgttgagt aagctcatgt
    22861 ctgtttcctt taaaaatatg tggcaaacaa gtgccgagta ttatagccaa ctcgcgccga
    22921 atgtctttgc ttgttttcgt cttcattttc ctttttcgcc agttttaaga cggtatacag
    22981 agcaggaaaa tccataaccg tagccgcatt tttcatagtg agatgaaagc gattacaaac
    23041 ttgtgattaa cgttttattt acttttttga agtgtgatgt aacgcaatcc gttacataac
    23101 gaattgtcta tagttttttc gcgaacatct tttcaccaat aataattacc ccgacgagga
    23161 caaccctatg tggaaacgct tacttgtagt ctctgcagtc tcggcagcca tgtcgtctat
    23221 ggcgttggcc gctccattaa ccgtaggatt ttcgcaggtc ggatcggaat ccggctggcg
    23281 cgctgcagaa accaatgtgg cgaaaagtga ggccgaaaaa cgcggaatta cgttgaaaat
    23341 tgccgatggt cagcaaaagc aggaaaacca gattaaagcg gtacgttcat tcgttgcaca
    23401 aggggtggat gcgatcttta tcgctccagt ggttgcgacg ggttgggaac cggtattaaa
    23461 agaggcgaaa gatgccgaaa tcccggtctt cttgcttgac cgttccatcg atgtgaaaga
    23521 caaatctctc tatatgacca ccgtcactgc cgacaacatc ctcgaaggca agttgattgg
    23581 tgactggctg gtaaaagaag tgaatggcaa accatgcaac gtggtggaac tgcagggcac
    23641 cgttggggcc agcgtcgcca ttgaccgtaa gaaaggcttt gccgaagcca ttaagaatgc
    23701 gccaaatatc aaaatcatcc gctcgcagtc aggtgacttc acccgcagta aaggcaaaga
    23761 agttatggag agctttatca aagcggaaaa caacggcaaa aacatctgca tggtttacgc
    23821 ccataacgat gacatggtga ttggtgcaat tcaggcaatt aaagaagcgg gcctgaaacc
    23881 gggcaaagat atcctcacgg gttccattga cggcgtaccg gatatctata aagcgatgat
    23941 tgatggcgaa gcgaacgcca gcgttgaact gacgccgaac atggcaggcc ccgcttttga
    24001 cgcgctggag aaatacaaaa aagacggcac catgcctgaa aagctgaccc tgaccaaatc
    24061 caccctttat ctgcctgata ccgcaaaaga agagttagag aagaagaaaa atatggggta
    24121 ttgagggttg ctatgcctga tgccgattcg taggccggat aaggcgctcg cgccgcatcc
    24181 ggcgatggtg cactgaagcc tgatgcgacg cttaccgcgt cttatcatgc ctactgggag
    24241 cacgctttac accgggggaa accatgacga ccgaccaaca ccaggagatc ctccgcaccg
    24301 aaggattaag taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc
    24361 gccgtggcga aatcatggcg ctgcttggtg aaaacggggc gggaaaatca acgctaatca
    24421 aagcattaac tggtgtatac cacgccgatc gcggcaccat ctggctggaa ggccaggcta
    24481 tctcaccgaa aaataccgcc catgcacaac aactcggtat cggcaccgtc tatcaggaag
    24541 tcaacctgct acccaatatg tcggttgctg ataatctatt tataggccgc gaacccaaac
    24601 gtttcggcct tctacgccgt aaagagatgg aaaagcgcgc caccgaactg atggcatctt
    24661 acggtttctc cctcgacgtg cgcgaaccgc ttaaccgttt ttcggtggcg atgcagcaaa
    24721 tcgtcgccat ttgccgggct atcgatctct ccgccaaagt gctgatcctc gatgaaccca
    24781 ccgccagcct cgacacccag gaagtggagc tattatttgg cctgatgcgt cagttgcgcg
    24841 atcgcggcgt cagcctgatc tttgtcactc actttctcga tcaggtctat caggtcagcg
    24901 atcggatcac cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacg cgcgagctac
    24961 cgcagatcga actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc
    25021 gtgccgggcg aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa
    25081 aaggaacgat agcaccgttt gatctcgaag tgcgccccgg cgagatcgtc ggtctggctg
    25141 gcttgctggg atccggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg
    25201 acagcggcac ggcgttgatc aaaggcaaac cgcaaaccct gcgatcgcca catcaggctt
    25261 cagtactggg catcggattc tgcccggaag acaggaaaac cgatggcatc atcgctgccg
    25321 cctcggtgcg ggaaaatatc atcctcgctc tccaggccca gcgcggctgg ctacgaccca
    25381 tttcccgcaa agagcagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca
    25441 caccttcaac tgaacaaccg attgaatttc tctctggcgg caatcagcaa aaagtgttgc
    25501 tttcacgttg gctactgacc cgaccgcaat ttctgatcct cgacgagcca acccgcggca
    25561 ttgatgttgg tgcccacgcc gagatcatcc gcctgattga aacgctgtgt gctgacggtc
    25621 cggcgctgtt ggtgatctcc tccgaactgg aagagctggt gggctatgcc gatcgggtga
    25681 ttatcatgcg cgatcgcaaa caggtggcgg agatcccgct ggcagagctt tccgttccgg
    25741 cgatcatgaa cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tctcccggac
    25801 actacgccgc cgaaaaggcg ctttcgctgg ccaacgggaa tgccgcagct ggcggcgcta
    25861 ttgctggtgc tgctggtcga tagcctggta gccccgcatt tctggcaggt ggtgcttcag
    25921 gatgggcgtt tgttcggtag ccctatcgac attcttaacc gtgcagctcc cgttgcgtta
    25981 ctggcgattg gtatgacgct ggtgatcgcc accggtggga ttgatctctc cgtaggggcg
    26041 gtgatggcta tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg
    26101 attgttttgt taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg
    26161 gtagcgatcc tcaaaattca gccgtttgtc gccaccctga tcctgatggt cgccgggcgc
    26221 ggcgtggcgc aactgatcac cgccgggcag atcgtcacgt ttaactcgcc ggatctctca
    26281 tggtttggca gcggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg
    26341 cttctcctgt tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagccgtt
    26401 ggtatcaaca ttcgggcggc aaaaaatgcc ggggtaaaca cgcggatcat cgtcatgctc
    26461 acctacgtgt tgagcgggct gtgtgcggcg attgcgggca ttatcgtggc ggcggatatt
    26521 cgcggtgccg atgccaataa cgccgggtta tggctggagc tggacgccat tcttgcggtg
    26581 gtgattggcg gcggatcgct gatgggcggg cgctttaacc tgctgctctc agtcgtgggg
    26641 gcgctgatta ttcaggggat gaacaccgga attttacttt ctggctttcc gccggagatg
    26701 aaccaggtcg taaaagcggt ggtggtgctt tgtgtgctga ttgttcagtc gcaacgcttt
    26761 atcagtctga ttaaaggagt acgtagccat gataaaacgt aacttgccgc tgatgatcac
    26821 catcggcgtc tttgtgctgg gctatctcta ctgcctgacc cagtttcccg gttttgcttc
    26881 cacaagagtg atctgcaata tcctgaccga taacgccttt cttgggataa tcgccgttgg
    26941 catgaccttt gtgatcctct ctggcggtat cgatctctcc gtcggttcgg tgatcgcctt
    27001 tactggcgtg tttctggcaa aagtgattgg cgatttcggc ctctcgccgc tgctggcgtt
    27061 tccgctagtg ctggtgatgg gctgtgcctt tggcgcattt atggggcttt tgatcgacgc
    27121 cctgaagatc ccggcattta tcattaccct ggcaggaatg ttctttttgc gcggcgtcag
    27181 ctatctcgtt tcagaagagt cgatcccgat aaaccatccc atttatgaca cgctctcaag
    27241 ccttgcgtgg aaaatccctg gcggcggtcg cttaagtgcg atgggactgc tgatgctggc
    27301 ggtggtggtt atcggcatat tcctcgcgca tcgtacccgt tttggtaatc aggtatacgc
    27361 cattggcggc aacgcaacgt cggcaaacct gatggggatt tctacccgca gcaccactat
    27421 tcgcatttat atgctctcca ccggactggc aacgctggcg gggattgtct tctcgattta
    27481 tacccaggcc ggatatgcgc tggcgggcgt aggtgtggaa ctggatgcta tcgcctcagt
    27541 ggtaattggc ggtacgcttt tgagcggtgg cgttggaacg gtattaggga cgctttttgg
    27601 cgtggcgatt caggggctaa ttcagactta tatcaacttt gacggtacgc tgagttcctg
    27661 gtggacgaaa atcgccatcg gcattttgct gttcattttt attgccttac agcgtggatt
    27721 aacggtgctg tgggagaatc gtcagagttc gccagtgaca agagtcaata ttgcgcagcg
    27781 ataaaaacgc ctctccgtgt ggagaggcgc aggagattac gcgtccggga attcacggat
    27841 aaagcgttcg acatcttcaa ccatatggtc gttgccgaca aagaacgaac ggcgctggtg
    27901 cagagtttcc gggatgatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc
    27961 ttgttccgcc aggaatgcca tcgggttgca ctcatacagc aaacgcagtt tgccgtccgg
    28021 gtggctggcg gtgcttgggt agagataaat accgcctttc agcaggttac ggtggaaatc
    28081 cgcgaccagt gaaccgatat aacgtgaggt atacgggcgg ttggtggatt tatcttcttc
    28141 ctggcagaat ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt
    28201 gatggagtag gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac
    28261 gcccagcgaa ggatcgtaag taaaggcgtg aacaccgcat ccggtggtgt aaaccagcat
    28321 ggtagaggag ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa
    28381 atcttcttcc gttaccggcg tgccaacagg cgtaacgcgg cggtagatgg agaaaatggt
    28441 accgacagag acgttaacat cgatgttgga cgagccatcc aggggatcca tcagcaccac
    28501 gtattttgcg tgttcacagc cttcaaagac gacaatctca tcttcttctt cagaggcaat
    28561 gcccgcaacg atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc
    28621 gagtttctgc tgaacctcgc cctgcacgtt ctcagcaccg ctggcaccca ggatatcaac
    28681 cagtcctgct ttgttgatat cgcgatggat aatcttggcg cccagtttta ttgccgacag
    28741 caaagcagtg agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc
    28801 acctaacgtt ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat
    28861 cttaacaaat aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt gcggattatc
    28921 ctgaaatgcg tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg
    28981 atagtgacgt atgcgcattc atattttagg aatttgtggc acatttatgg gcggtctggc
    29041 gatgctggcg cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc
    29101 gatgagcacc ttacttgaga agcaaggcat tgagctgatt cagggttacg atgccagcca
    29161 gctcgatccg cagccggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg
    29221 tgtggaagcg gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga
    29281 ttttgtgctg cgcgaccgct gggtgctggc cgttgccggt acacacggca aaactaccac
    29341 cgcgggaatg gcgacctgga ttctggaaca gtgcggttac aaaccgggat ttgtgatcgg
    29401 cggtgtgccg gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat
    29461 cgaagcggat gagtatgact gcgccttctt cgacaaacgc tctaaatttg tccattactg
    29521 cccgcgtacg ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct
    29581 gaaagcgatc cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggccgtat
    29641 tatctggccg gaaaatgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga
    29701 gcaggagctg gtgggtgagc agggtcactg gcaggcgaaa aagctgacca ctgacgcttc
    29761 tgaatgggaa gtcttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtggg
    29821 cgaacataat atgcacaatg gcctgatggc gattgcggcg gctcgccatg ttggtgtagc
    29881 gcctgcagat gccgctaacg cgctgggttc gtttattaac gctcgtcgcc gtctggagtt
    29941 gcgtggtgaa gcgaatggcg tgacggtata tgacgatttt gcccatcacc cgacggcaat
    30001 tctggcaacg cttgcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt
    30061 gctggagccg cgctcgaata ccatgaaaat ggggatctgc aaagacgatc tggcaccttc
    30121 attaggtcgt gccgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc
    30181 agaagtggca gaagcctgcg ttcagcctgc acactggagt ggcgatgtgg atacgctggc
    30241 agatatggtg gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaacggcgg
    30301 ttttggtggg atccatcaga aactgctgga tggtctggcg aagaaggcgg aagctgcgca
    30361 gtaattcggc ctcagcctga tatagtgtgc gtaggccgga taaggcgttc acgccgcatc
    30421 cggcatttga gcatagtgcc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc
    30481 accgcatccg gcaaaccata ctcacatcaa caacgaaaat tacccttcat tctccgccag
    30541 ttcgcgcaga tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc
    30601 tttcttcgcg ttgcggatca gagtacgcag ctgctgacga tcggcatcgg gccacagatt
    30661 taatacctca gcgatggcgt catcaccttg atcgatcaga cgatcgcgca ggttttccag
    30721 tttatggaac agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg
    30781 gatcggttct acatcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg
    30841 accttccatc ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg
    30901 gatcttatcc agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc
    30961 ctcggcatca cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc
    31021 gatgtcatca ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc
    31081 cttaaaaaaa gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta
    31141 gacttcagag aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa
    31201 gcgcagcgca agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa
    31261 tcggacggtg cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat
    31321 ggtgaagtgg agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac
    31381 cagaaccgca aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact
    31441 gtacaggcgg cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca
    31501 gacaaagagc tgctggcctt tgacgcacca gatctcgact tgttccaccc tgcggaagtt
    31561 tccccggatg aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac
    31621 aaacgcatta ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt
    31681 ggcaacagcc acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt
    31741 gtaattgccg aagaaaatgg cgatatggag cgtgattacg cctacaccat tggccgcgcg
    31801 atgagcgatc tgcaaacgcc agagtgggtc ggggccgact gtgctcgccg tactttatcg
    31861 cgcctgtcac cgcgtaaact ctccaccatg aaagcgccag tcatttttgc caatgaagtg
    31921 gcaaccgggc tttttggcca tctggtgggg gcgatagcgg gtggctcggt ttaccgtaaa
    31981 tctacctttc tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa
    32041 gagcatccgc atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc
    32101 accgagcgtc gcgatattat taaagatggc atcctgactc agtggctgct gaccagctac
    32161 tcggcgcgga aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg
    32221 attgccggac aagggctaag cttcgaacaa atgctcaaag agatgggcac cgggctggtg
    32281 gtgacggagc tgatgggcca gggcgtgagt gccattaccg gtgattattc ccgtggtgca
    32341 gcgggcttct gggtagagaa tggcgaaatt cagtatcccg ttagtgaaat caccatcgca
    32401 ggtaatttaa aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc
    32461 agtaatatac agtgtggttc tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa
    32521 actggcgcga cctgccgcgc tataaactaa aaaattacac aaaatcattc gcactgcatc
    32581 gagacggaaa gtgaatgagg gccggggagc gtactcgcag tacgtgaccc gggcgaatga
    32641 gcgtagccgt cgatgaggca gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa
    32701 tgcgtaaaag cctgttagct attcttgcag tctcctcgtt ggtattcagt tctgcgtcgt
    32761 ttgccgctga tcttgaagac aatatggaaa ccctcaacga caatttaaaa gtgatcgaaa
    32821 aagcggataa cgcggcgcaa gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg
    32881 atgcgcaaaa agcaacgccg ccgaagctcg aagataaatc accggacagc ccggaaatga
    32941 aagatttccg ccacggtttc gacattctgg tcggtcagat tgacgacgcg ctgaagctgg
    33001 caaatgaagg taaagtaaaa gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca
    33061 acgcctatca ccagaagtat cgttaattcc tcatttccct gttgcctgca ctcaggtaac
    33121 agggaactta tcatcgcaaa tgatgcacca cctgattgct gctgccgcgc cagatcaggg
    33181 aagggtcttt taaatcctgc acaaatttgc cgtcgaccag tacgttaatc aaatcaacaa
    33241 cctgcatttg cgcagcgttg agttcgtcga gtttatagcc tgtccacacc cagatgtctt
    33301 tacccggaca ctcggcgcgg atgcgtttta ccagtttcag aatatccggc acgttttgcg
    33361 gatgcagcgg atcgccgccg gagagggaaa taccctggcg tttgatacga gtgtcattca
    33421 gatcgttaat aatctggtct tccattgctt tagtaaatgt ctgaccggaa tttacccgcc
    33481 atgtgctttt gttatagcaa ccggggcatt catgaacaca cccggagaca aacagggtgc
    33541 agcgagtgcc ggggccgttg acgatgtcga caggatagta ctgatgataa ttcattgttt
    33601 cgcctggctt atcagtccgc gtataggatg catccggtaa tcaacgcctg atgcgacgct
    33661 gtcgcgtctt atcagaccta caatcgttgt tgccatgtag gccggataag gcgtttacgt
    33721 cgcatccggc atttgcttaa cgtagagtga agattaacct atctgcccat tccccaaatg
    33781 tttaacgcgg cgcttaactt cttcctgctt accggcgtta aatggacgtg catccgggct
    33841 acctaaatat ccgcacacgc ggcgagtcac cgacacacgg gaggcgtcat ggttaccaca
    33901 tttcgggcaa gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc cacactcgta
    33961 gcactcatca atcggtgtat tggtgccgta atacggtaca tgctgatagc tgtaatccca
    34021 gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa
    34081 accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac
    34141 cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg
    34201 caccacgcca aactcagcag tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg
    34261 cgtgctgtag agactgaaac cataacccgt ttcttctttc cactgatcca ctgcctggcg
    34321 cagacgttca acaatggcga tacctttcgc gcgaagctgc tcgttgtcgt aaacatgctc
    34381 gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg aaatagacgc
    34441 acgaccgttt ttgaagattt cagaaacatc atcgtcagca ttcagacgta cgccacaagc
    34501 accttccata tagaggatcg gggccacgcg cgctttcacg ccttcgagac gagcgatacg
    34561 ggtcatcagc gccttacgtg ccagcaccag acgttcatcc agcagcttcc agaaggtggc
    34621 ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca cgccgaggtt
    34681 gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga ggaagctgcg
    34741 gcagcccatc ggggttttaa acgaaccggt gactttcact acctgatcgt agttcaggat
    34801 atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg
    34861 atcgcctttt ttatggttca gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc
    34921 agttttacgg tttttaccga ggcctgcgat acggttacgc aggatcgatt cctgaatcag
    34981 gcgcgattcc cagctggtgc ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt
    35041 ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt agcactcttt
    35101 gatggtacga gagtttgcgt agccttcggc gtccgggatg ttccactctt ctgcggtctt
    35161 gcgatgtttg ttgtagctgg cggtaacaaa cggcgccagc acttcatcga tacggttaat
    35221 ggtggtgccg ccataaatat ggctggcgac ctgagcaata atctgcgcag ttaccgcggt
    35281 tgccgtagag atcgacttcg gcggttcaat ctcggcgttc cccatcttaa agccctgggt
    35341 cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaagaacg gcgagtaatc
    35401 gagatcgtga tagtgaatat cgccacgctc atgtgcctgc accacgtcac gcggcagcag
    35461 gtgctgacgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct gggttggaat
    35521 caccttgctg tctttgttgg cgttttcgtt gagtaacgag gcgttggtct gctcgaccag
    35581 accacggatc tcctggttca ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg
    35641 gtactcgatg taagcacgag ccagttgttt gtatggaccc gacatcagct ggttttcaac
    35701 tgcggtctgg atctcgttga tatccacctg gttgcggccc tgcatctgct cgctgacaac
    35761 cgcggcaaca gtggcgcaat aatcggcatc atcgacttcc gctgctttag ctgcacgcag
    35821 aattgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc gtttcatcac
    35881 atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa attgggaaag
    35941 gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc
    36001 cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga ttaatacaac
    36061 atattgggtt ggggcgcatt ttaaagtcta tatatagtgc tttgcatcaa ggatgtttta
    36121 actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg cgctacagcg
    36181 ttatgtaaat tttttaatga atttgctggt tgaaaaatca acaaaaagaa catgctgaca
    36241 gacaaaaccc cggaatgacc ggcattaccg gggcttaggg aagatttact tctgtaacca
    36301 ccagacagcc tcaaaaggcc gtaaattcat ggcacagggt tgtggtgagg cttcttcgta
    36361 gttatgcatc acaagctgcc agttgccgcg catttgccct ggctgccagg gttggatctc
    36421 acggctaagg ttggcaatga ccagcaaggt ttgccccttc cattcacggc gatagcacca
    36481 caatacaggg ctgtttggca gcagatcctg gtaattgccc catgtcagga tggcttcttg
    36541 cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggagtcat cggccagcgc
    36601 ggcttctacg ttgatttgtt gatagttatc gcccaggcca atccacggtt cgccagccgt
    36661 aaacccggca ttatcgccgt tgctccattg catgggcgtg cggctgttgt cacgggattt
    36721 actggcgagg attgccaata actcgtcggc atcacgacca tcgttgcgca gctcggcaaa
    36781 catattgagg ctctccacgt cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat
    36841 gccaatctct tcgccttggt agatatacgg cgttccctgc atgccatgca gcaccatcgc
    36901 cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag aaacaatgcg
    36961 cggctgatcg tggttacacc agaacaaggc attccatgcc acgttgtgca ttccttgttg
    37021 ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc
    37081 accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc
    37141 tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt
    37201 cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg
    37261 tggcccgtcg gtgtagaagc gacgcccgtc gccatccagg tcttcaggga aacgcgggtc
    37321 tttggagatc agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa
    37381 ttcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg
    37441 ttctggtgca aagagatgca aatagtactg ttcgctttcc gcatgccagc gccacgcact
    37501 accgccaaat tttgaacgcc agttgttcgg tggcgtttct ggttcgccat cgcgccagat
    37561 ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt
    37621 agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt
    37681 caccagttcg tcaaaatcgt ccagcgtgcc gtagatggga tcaatcgccg tatagttcgc
    37741 tacgtcgtaa ccgttatcga cctggggaga gatataaaag ggggttagcc agatggcatc
    37801 aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc
    37861 gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca
    37921 ccagtgggga agattagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc
    37981 ccgaagaaaa ttaaacaata tccagcgtgc ccaggcggta tttccgctga tagataaacg
    38041 aggtgagtac aatcgggatg atgatggcga taaccattgc cagcgcaaat acctgccagt
    38101 agctcggttg aatcgagaga attcccggca ggccgcctac gccgatacca ttcgccataa
    38161 cgccgttcag gccgcatagt aatcctgcca gaccagaacc aatcatcgcg cacagcatcg
    38221 ggaagcgata tttcaggttg atgccgtaca ttgcaggctc agtgacccca agccaggcgg
    38281 agatagcggc aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatgc
    38341 ctatcacggc ggagccctga gcgatattcg acagcgcaat cagcggccac actggcgtgc
    38401 cacccatgct ttgaatcatc tgcaaatcaa tagcaagggt ggtctggtgt acaccggtga
    38461 tcaccagcgg ggcgtacagg aagccaaaca atgctgcgcc aatcggagca aagctgccgg
    38521 tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa
    38581 tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat
    38641 agaggtaatc cggcacgatg cgtttaaggc gagtttcaat aacgcccagc gccagcccgg
    38701 ctaacagtgc cgggatcacc tgcgcctgat agcctacttt ggcgatgctg aacatgccaa
    38761 agtcccacac ttccggcagc tgctgcccga gcagataagc gttcatcagc tggggagaaa
    38821 ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc gcccattttt ttcaccgctg
    38881 accagcaaat accgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat
    38941 cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ctgaccgttg ctcatgggca
    39001 aatcgccgat cacattgcga aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca
    39061 gcgggaagaa gatctccgcg aaatgagaga tcaactgctc atgccatttc atattctgcc
    39121 gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac gcaatcagtg
    39181 cttgatagta atcacccaca ttggtgccaa tcaccacctg aaattgcccg gcattggtga
    39241 aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt
    39301 tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc
    39361 cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc
    39421 atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg
    39481 gaacgttccc gaagtgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg
    39541 gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgtgc gatcaattgg
    39601 caggccgcct ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat ctccggatga
    39661 aggaatttca ttaatggcgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc
    39721 tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta
    39781 gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc aaggcctggc
    39841 agagcggcaa cgggattcag tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt
    39901 cgcttcccgg tcgtcacgtc actatgcggc acgccgagat aactgatatt acgatgcccc
    39961 tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa
    40021 gcaaaacctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt
    40081 tcttctgtta tgccagtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc
    40141 acccccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat gattgggtcg
    40201 taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa
    40261 tccaggcggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca
    40321 gagcgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc gcgggtgcgc
    40381 tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt
    40441 aagcgtgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg taacgtgttg
    40501 tttaattatt tgagcctaac tttacccgtg cattcagcaa tgggtaaagt ctggtttatc
    40561 gttggtttag ttgccagcag gtattatatc gccatagatg ctacgaatat tattggattc
    40621 tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg
    40681 cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg
    40741 gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc
    40801 ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt
    40861 ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt catcgcgatc
    40921 cattgcctgg cgcgcagcag acagtgaata cggcggtccc gccgtcctta agtgcgcatt
    40981 gcctgaaaat ggcggtgatg cccgaagaag aattatggaa aacgttcgac acccatccgg
    41041 aagggttaaa tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac
    41101 ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta
    41161 atattttact caccattctc ggcgctattt cttacgccac ggaagattta tttgccgcgg
    41221 gcgttatcgc gctaatggtc gctatttcta cgttgctgaa ctttattcag gaagcacgtt
    41281 ccactaaagc ggcagatgcc ctgaaagcga tggtcagcaa tacagcgacg gttctgcgcg
    41341 taattaacga caaaggcgaa aatggctggc tggagatccc gatcgaccag ctggtgcccg
    41401 gcgatataat aaaactggcg gcgggggata tgatcccggc agatttacgt atcttgcagg
    41461 cgcgggatct gttcgtcgct caggcgtcgt taaccggtga gtctctgccc gtagaaaaag
    41521 ccgctaccac tcgccagccg gagcacagca atccgctgga gtgtgacacc ctgtgtttta
    41581 tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgctaca ggtgccaata
    41641 cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc
    41701 agcaagggat cagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggctccgg
    41761 tggtgctgtt aatcaatggt tacaccaaag gcgactggtg ggaagcggca ctgtttgcgc
    41821 tttcggtcgc ggtaggctta acgccggaaa tgttgccgat gattgttacc tcgacgctgg
    41881 cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt caaacatctg gatgctattc
    41941 agaactttgg cgcaatggat attctgtgca ctgataaaac tggcaccctg acacaggaca
    42001 aaattgtgct ggagaatcat accgatatct ccggtaaaac cagcgaacgc gtgctgcata
    42061 gcgcgtggtt gaacagtcat tatcagaccg gacttaaaaa cctgctcgat acagcggtgc
    42121 tcgaaggtac ggatgaagag tcagcgcgct cgctggccag tcgttggcaa aaaattgatg
    42181 agattccgtt tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa aataccgagc
    42241 accatcagct ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc
    42301 gtcacaatgg cgagattgtg ccgctcgatg acatcatgct gcgtaagatt aagcgggtta
    42361 ctgatacgct taatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag
    42421 cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg
    42481 ctttccttga tccgccaaaa gagacaaccg ctccggcact gaaggcatta aaagcgagtg
    42541 gaatcaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg
    42601 aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg
    42661 acgaactggc aaatctcgca cagcgcacca cgctgtttgc ccgcctgacg ccgatgcata
    42721 aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg
    42781 gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg
    42841 tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg
    42901 aagagggggt tattgaagga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga
    42961 cggcgagctc caacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct
    43021 tcctgccgat gttgccgtta cacttgctta ttcagaacct gctgtatgat gtgtcacagg
    43081 tggcgatccc gtttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc
    43141 cggcggatct ggggcgcttt atgatcttct tcggaccgat cagctcgatc ttcgatattc
    43201 tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt
    43261 tccagtcggg atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc
    43321 gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg
    43381 tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcagc
    43441 tgcaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca gggtatatga
    43501 cattaactca gttggtgaaa ggattttata gccgtcgtta cggctggcag taaagaataa
    43561 actgggcacg atagccccga ttcagattgc agacaaagta agctttgttc atgccggatg
    43621 cggcgtaaat gccttatccg gccaacaaat tcaataaatt gcaggaatca cgtaggcctg
    43681 ataagcgtag cgcatcaggc gattttgcgt ttgtcatcag acttaatccg ggcatgatag
    43741 cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa tcttcacgtc
    43801 tttcggcaga cgggcaactt caacgcaaga acgtgccggg aaggtggcat tgtgttcggt
    43861 gaagaaggct tcgtaggtag cattaacggt tgcgaagtcg ttcagatctt tcacaaacac
    43921 ggtggtttta acgatgtcgc ccactttcag gcctgcggct tcgacgatcg cttttacgtt
    43981 atccagcgac tgacgtgcct gtgcagcgac gtctgccggt acttcgcccg ttttcggatt
    44041 taccgggatc tggccggagg tgatgatcat attgcccaga tcaacgccct gtacgtaagg
    44101 accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt ctcctttatt
    44161 acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca accgcaatta
    44221 attggccagc accacattat gggaaaactc tttttcacag tatttgcatt tgagcgcgat
    44281 gtcattggcg cgttttcgca cggcaaagct ggaagaaacc ggttcggcat ggctgataca
    44341 gttgctgttc gggcagacca gcacattgtc gatgcgctct ggcagactgg ggcgcgattt
    44401 acccaccact tcatagttgt cgatacggtt aaccgtggct tgcggcgcat acaatgccag
    44461 ttgatctacc tgatcttcac tcaaaaaggt attttcgatt ttgatcagat ctttgcggcc
    44521 catctcgcca gaaggcaagt tcagaccgat ggtgatgcgc tgatccgttt cggtcagctt
    44581 gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta ccgtgccgcg
    44641 tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc cttacagtac
    44701 cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc cgttgcctgc
    44761 ctgctggaag taccaggcgt gtggcgtttt atcaacatcc gtcgcaatct catcaacacg
    44821 cggcagcgga tgcagcactt tcatattggc tttggcgttg tggagatcgc tggcgcgaag
    44881 aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt tttgcacgcg
    44941 ggtcatgtac aggatgtcta cttccgccat cacttcttca atagagctgt gcagactcca
    45001 tgcgatccct ttttcatcga gcatatccag aatgtattgc ggcattgcca gcgcgtccgg
    45061 cgcgatgaag taaaaacggt tgccgtcgaa cttcgctaac gcctgagtca gggagtgaac
    45121 ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca gacgcccctg
    45181 ggtttcctga atagtgaata agtccagcaa ggtttgcgtc ggatgttggt tggagccatc
    45241 accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg ccgcaccttc
    45301 ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa tggtatcggc
    45361 cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc ccaccacgct
    45421 ggcacccagg cggtgcatag atgtttcgaa agagaggcgg gtacgggtag aggcttcgaa
    45481 gaaacagctg gcaatgactt tgtgcttcaa cagctctggt tgcgggtttg ctttcagttt
    45541 cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta tggaaatgat
    45601 atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc aaaaaaaagc
    45661 ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca ggccagcgtc
    45721 ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc ctcccggcaa
    45781 attgtccggc attatactca tcgtcagaag cggtgcaagc atttgatgca atattttgtc
    45841 agcgcaaacg gtttatttga attaaaagtc aaggtatatg catttttatt tgatgtgatt
    45901 ctgcagggga actgtccttc ggtatcaata attgcagaca ttccctgctt tttccttttt
    45961 cactttcacg caatcagata tgcattttat tccattcatc cgacttatag ggcggagtgt
    46021 gaaagcgaac ggctaacact attgcttact gctcagggat gcgcgctatc actttaattt
    46081 caaaatcaaa gcctgccagc catgtaacac ccaccgccgt ccagtttgga taaggtgggg
    46141 cgctaaatat ttcatttttc accgtcatga tgtcttcaaa ttggttttct ggatcggtat
    46201 ggaagctcgt aacatcaatg atatcgtcaa aagtgcatcc cgcagctgcc agggtcgcat
    46261 gcaaattatc aaatgccagt ctgacttgtt gctgaaaatc gggttctggt gttccgtcct
    46321 ctcgacttcc tacttgcccg gaaacaaaca gcaaatcgcc ggaacgaata gccgcagaat
    46381 aacgatgctc agcatatagt gaatgtcggc cagcagggaa aacagcggtt ctttctacca
    46441 tttggttatc ctcaagattt acgacatgaa cagaagattt ctctttaccg ggagccgctt
    46501 ttagcggacg acgtgagtaa acaaaaccca gacatcatgg ataatggctg ggcttaattg
    46561 agcgtagtcg gttatgcgcc aaacgcgcca tcaatggtat gcatcgcgcc ggtaacaaaa
    46621 ctggcttctg gccctgctaa ccatgcgacc ataccagcga cctcttccgg ttgcccatgt
    46681 cttttgatag ccatcaaact atgcaacata tcgcgcattg gcccgttggc gggattagcg
    46741 tcggtatcaa ttggccctgg ctggacgacg ttaatggtga tcccacgcgg tccaaaatca
    46801 cgggccagcc cgcgcgccat gccttgcagg gcagatttgc tggcggcata agcagccatg
    46861 cctgcaacag gcatacgatc gccattcacg gagccgatga ttaagatgcg cccgccttcg
    46921 ggcatctgcc gggcggcttc aacagaggca tgataaggag catgaatatt gattttgaaa
    46981 aggcgatcaa tatcgtcggc atttaattcc agggcctcgc caaagacgcc aatacctgca
    47041 tttaccacca ggatatccaa tgcgccgctc ttacgaacga catcaatgac agcgtctctg
    47101 tcagcactat ctgtgaatac tgctgtcgct ccagtctctt gtgccaggcg tttagcggca
    47161 tctttcgacc ccgcataggt gaatcgtaca ttggccccat cggtgacgaa acgacgtacg
    47221 atagcggcac cgataccacg actgccaccg aggatgagaa ctgtcttacc tgtaaaagcg
    47281 cccataagga ctccttgatt tattatgtaa catgcattac aaaactgttt taactttctg
    47341 tcaacaagtt ttgtaatggt cactaaaaaa caatctcgcg ttccaggtcg tcccagacgt
    47401 ttcgctcctg agcaggcagt ctctgcggca aaagtgcttt ttcaccaaaa aggtttcgat
    47461 gctgtcagtg ttgctgaagt tactgattat cttggtatta accccccgag tctctacgcg
    47521 gcttttggca gtaaagctgg gttatttagc cgtgtactca atgaatacgt cggtacggaa
    47581 gctattccgc ttgccgatat tcttcgtgat gatcgtccag taggcgagtg cctggttgag
    47641 gtattaaaag aagcggcgcg cagatatagc caaaacggcg gctgcgctgg ctgtatggtt
    47701 cttgaaggta ttcatagtca tgatccacta gcgcgtgata ttgccgttca atattatcac
    47761 gccgcagaaa cgaccattta tgactatatc gccaggcggc atccacaaag cgcacaatgt
    47821 gtgactgatt ttatgagtac cgtgatgtca gggctttctg cgaaggcacg ggaggggcac
    47881 tcaatcgaac aactctgtgc aacagctgca ctggcggggg aagcgataaa aactcttctc
    47941 aaggagtgat gctggccttg atccgaaagg cgggaacgcg cctgccgata agttgtgata
    48001 agacaataat tcacgcgtta aggctggcgg aattgatcat cttttcgtat aacgatagaa
    48061 atgaaacatt gttttaatta aggagtggaa aagatgatca tcggaaatat tcataatctt
    48121 cagccgtggc taccccagga gttacgccag gcgattgagc atatcaaagc gcacgttacg
    48181 gcagaaacgc caaagggtaa gcacgatatc gaaggcaatc gtctgtttta tcttatctcg
    48241 gaagatatga ccgagccgta cgaagctcgc cgtgcggagt atcatgcccg ctatctcgac
    48301 attcagattg tgttaaaagg tcaggaaggc atgaccttca gcacgcaacc tgcaggcacg
    48361 ccggataccg actggttagc tgataaagac atcgcatttt tgccggaagg cgttgatgag
    48421 aaaacagtta tccttaatga aggtgatttt gttgtgtttt atccggggga agtgcataaa
    48481 ccgctgtgcg cagtgggcgc gccagcccag gttcgcaaag cagtagtgaa gatgctgatg
    48541 gcgtgatgac ttttcgccgt aaataactcc aggtttacgg cgagtttgtg aaaagagcgt
    48601 tttttgatat ttttttgtga gtagaatttg taatacttag acgttcttat taactcaagg
    48661 agtccgtcat gagcaaaata tcaggttgga atttttctca aaacattaca tcagccgaca
    48721 attgtaaaca aaaaaatgaa gacttagata cctggtatgt gggaatgaat gattttgccc
    48781 gaattgccgg agggcagaat agcagaagca atattctttc tcccagagca tttttggagt
    48841 ttttggctaa gatatttacc ctgggttatg tggattttag caaacgctcc aacgaagcgg
    48901 gtagaaatat gatggctcat attaagtcct catcttatat caaaaataat gatggtagtg
    48961 agataatgaa gtttgttatg aataatcctg aaggggagcg agcggattta tctaaggtgg
    49021 aaatagaaat tacacttgcc tcagctttta ataatgggat tcgtgaagga catacagtca
    49081 ttatatttac acaacctgat ggttcgacta atcgttatga agggaagtcc tttgaaagaa
    49141 aagatgagag ttcattacac ctgattacta acaagattct ggcgtgttac caacgcgaag
    49201 ctaacaagga aatagctcgt ctattaaata ttccgcagga gttaaataat tctcaggact
    49261 tgaataactc gcaggtgagt tgtaaagatt cagttgattc tacgattagg gatttattag
    49321 aaaaaccatt gaataatgca ttattagcaa taaggaaaga acatctgcta ttaatgcctt
    49381 atgtatgtaa tgaatcgatt tcatacttgc ttggtgaaaa agggatactg aaagaaatag
    49441 atgaccttaa cgcagtaaat aattatcttc tt
//
LOCUS       DAZUFB010000025        48779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZUFB010000025 DAZUFB010000000
VERSION     DAZUFB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.025"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            425..1642
                     /gene="alaA"
                     /locus_tag="ACHY3M_001663"
     CDS             425..1642
                     /gene="alaA"
                     /locus_tag="ACHY3M_001663"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFS4316596.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHY3M_001664"
     CDS             1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHY3M_001664"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFS4316597.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2384..4216)
                     /locus_tag="ACHY3M_001665"
     CDS             complement(2384..4216)
                     /locus_tag="ACHY3M_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS4316598.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHY3M_001666"
     CDS             complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHY3M_001666"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFS4316599.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4964..5458)
                     /locus_tag="ACHY3M_001667"
     CDS             complement(4964..5458)
                     /locus_tag="ACHY3M_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4316600.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHY3M_001668"
     CDS             complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHY3M_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFS4316601.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6334..7536
                     /gene="ackA"
                     /locus_tag="ACHY3M_001669"
     CDS             6334..7536
                     /gene="ackA"
                     /locus_tag="ACHY3M_001669"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFS4316602.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7611..9755
                     /gene="pta"
                     /locus_tag="ACHY3M_001670"
     CDS             7611..9755
                     /gene="pta"
                     /locus_tag="ACHY3M_001670"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4316603.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPS
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGCSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHY3M_001671"
     CDS             9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHY3M_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4316604.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHY3M_001672"
     CDS             complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHY3M_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFS4316605.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHY3M_001673"
     CDS             complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHY3M_001673"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFS4316606.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHY3M_001674"
     CDS             complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHY3M_001674"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFS4316607.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHY3M_001675"
     CDS             13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHY3M_001675"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFS4316608.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14189..14551
                     /gene="folX"
                     /locus_tag="ACHY3M_001676"
     CDS             14189..14551
                     /gene="folX"
                     /locus_tag="ACHY3M_001676"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFS4316609.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14572..15465
                     /locus_tag="ACHY3M_001677"
     CDS             14572..15465
                     /locus_tag="ACHY3M_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFS4316610.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHY3M_001678"
     CDS             complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHY3M_001678"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFS4316611.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHY3M_001679"
     CDS             complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHY3M_001679"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFS4316612.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17381..18097)
                     /locus_tag="ACHY3M_001680"
     CDS             complement(17381..18097)
                     /locus_tag="ACHY3M_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4316613.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHY3M_001681"
     CDS             complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHY3M_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFS4316614.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHY3M_001682"
     CDS             complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHY3M_001682"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFS4316615.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHY3M_001683"
     CDS             complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHY3M_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFS4316616.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHY3M_001684"
     CDS             complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHY3M_001684"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFS4316617.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIKTHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHY3M_001685"
     CDS             complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHY3M_001685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4316618.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHY3M_001686"
     CDS             complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHY3M_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFS4316619.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23886..24548)
                     /gene="dedD"
                     /locus_tag="ACHY3M_001687"
     CDS             complement(23886..24548)
                     /gene="dedD"
                     /locus_tag="ACHY3M_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFS4316620.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24538..25806)
                     /gene="folC"
                     /locus_tag="ACHY3M_001688"
     CDS             complement(24538..25806)
                     /gene="folC"
                     /locus_tag="ACHY3M_001688"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFS4316621.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25876..26790)
                     /gene="accD"
                     /locus_tag="ACHY3M_001689"
     CDS             complement(25876..26790)
                     /gene="accD"
                     /locus_tag="ACHY3M_001689"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4316622.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26946..27605)
                     /locus_tag="ACHY3M_001690"
     CDS             complement(26946..27605)
                     /locus_tag="ACHY3M_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS4316623.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27688..28500)
                     /gene="truA"
                     /locus_tag="ACHY3M_001691"
     CDS             complement(27688..28500)
                     /gene="truA"
                     /locus_tag="ACHY3M_001691"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4316624.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28500..29513)
                     /locus_tag="ACHY3M_001692"
     CDS             complement(28500..29513)
                     /locus_tag="ACHY3M_001692"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4316625.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29579..30715)
                     /gene="pdxB"
                     /locus_tag="ACHY3M_001693"
     CDS             complement(29579..30715)
                     /gene="pdxB"
                     /locus_tag="ACHY3M_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFS4316626.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30814..31809
                     /gene="flk"
                     /locus_tag="ACHY3M_001694"
     CDS             30814..31809
                     /gene="flk"
                     /locus_tag="ACHY3M_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFS4316627.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31806..32984)
                     /locus_tag="ACHY3M_001695"
     CDS             complement(31806..32984)
                     /locus_tag="ACHY3M_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4316628.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33268..34488)
                     /gene="fabB"
                     /locus_tag="ACHY3M_001696"
     CDS             complement(33268..34488)
                     /gene="fabB"
                     /locus_tag="ACHY3M_001696"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFS4316629.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34647..36653
                     /gene="mnmC"
                     /locus_tag="ACHY3M_001697"
     CDS             34647..36653
                     /gene="mnmC"
                     /locus_tag="ACHY3M_001697"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFS4316630.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVGVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36774..37052)
                     /locus_tag="ACHY3M_001698"
     CDS             complement(36774..37052)
                     /locus_tag="ACHY3M_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFS4316631.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37086..37634)
                     /gene="epmC"
                     /locus_tag="ACHY3M_001699"
     CDS             complement(37086..37634)
                     /gene="epmC"
                     /locus_tag="ACHY3M_001699"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFS4316632.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37634..38443)
                     /locus_tag="ACHY3M_001700"
     CDS             complement(37634..38443)
                     /locus_tag="ACHY3M_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4316633.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38443..39267)
                     /gene="mepA"
                     /locus_tag="ACHY3M_001701"
     CDS             complement(38443..39267)
                     /gene="mepA"
                     /locus_tag="ACHY3M_001701"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFS4316634.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39271..40356)
                     /gene="aroC"
                     /locus_tag="ACHY3M_001702"
     CDS             complement(39271..40356)
                     /gene="aroC"
                     /locus_tag="ACHY3M_001702"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4316635.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40391..41323)
                     /gene="prmB"
                     /locus_tag="ACHY3M_001703"
     CDS             complement(40391..41323)
                     /gene="prmB"
                     /locus_tag="ACHY3M_001703"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4316636.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41489..42040
                     /gene="smrB"
                     /locus_tag="ACHY3M_001704"
     CDS             41489..42040
                     /gene="smrB"
                     /locus_tag="ACHY3M_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFS4316637.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42113..42964)
                     /locus_tag="ACHY3M_001705"
     CDS             complement(42113..42964)
                     /locus_tag="ACHY3M_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFS4316638.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42966..43505)
                     /locus_tag="ACHY3M_001706"
     CDS             complement(42966..43505)
                     /locus_tag="ACHY3M_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4316639.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43502..43990)
                     /locus_tag="ACHY3M_001707"
     CDS             complement(43502..43990)
                     /locus_tag="ACHY3M_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4316640.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43987..44496)
                     /locus_tag="ACHY3M_001708"
     CDS             complement(43987..44496)
                     /locus_tag="ACHY3M_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4316641.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44512..45264)
                     /locus_tag="ACHY3M_001709"
     CDS             complement(44512..45264)
                     /locus_tag="ACHY3M_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4316642.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45284..47929)
                     /gene="yfcU"
                     /locus_tag="ACHY3M_001710"
     CDS             complement(45284..47929)
                     /gene="yfcU"
                     /locus_tag="ACHY3M_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFS4316643.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(48011..48574)
                     /locus_tag="ACHY3M_001711"
     CDS             complement(48011..48574)
                     /locus_tag="ACHY3M_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4316644.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11934 a        13200 c        12170 g        11475 t
ORIGIN      
        1 tattaaataa taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc
       61 acaccttgcc cccattacga cacctcggaa atcatcacgc tgttaaactc tccgtaatgc
      121 ggaaatatca tcatcgcgtt tcattagtga attcttaaat gaggcatttt cacacaatta
      181 tcttacagat aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat
      241 tgcatgtgaa gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc
      301 gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag
      361 tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag
      421 attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg
      481 tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa
      541 catcggcaac ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat
      601 acgcaacctg cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa
      661 agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta
      721 catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg
      781 ggacgaaatg ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc
      841 cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga
      901 tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa
      961 cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca
     1021 gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctttacg acgacgctga
     1081 gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc
     1141 gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa
     1201 acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa
     1261 cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt
     1321 tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga
     1381 tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga
     1441 cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga
     1501 aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat
     1561 tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct
     1621 ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagcgtt
     1681 atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat
     1741 ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg
     1801 cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca
     1861 gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg
     1921 atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc
     1981 aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc
     2041 gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat
     2101 agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt
     2161 ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg
     2221 acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc
     2281 ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc
     2341 gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca
     2401 tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca
     2461 caaagtcact gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa
     2521 ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg
     2581 acacccccat cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg
     2641 tattagagat aaatagccca ataaccgccg acaagacaaa caaacacccc agcatcatat
     2701 gtggcccgta accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg
     2761 ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt
     2821 gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg
     2881 cgatgatagc ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca
     2941 ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt
     3001 ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc
     3061 agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt
     3121 ccagcgccac gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac
     3181 caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca
     3241 tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca
     3301 attgctcact acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa
     3361 cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc
     3421 gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca
     3481 gccgttgacc tatcattggg gatccggggc gaatagccag acgtcgcgca cgcccggtca
     3541 gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg
     3601 tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca
     3661 gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca
     3721 gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa
     3781 tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg
     3841 caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc
     3901 caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcca
     3961 ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa
     4021 taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg
     4081 ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca
     4141 tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa
     4201 tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata
     4261 aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc
     4321 gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc
     4381 attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag
     4441 gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
     4501 cagcagctgc gcacctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
     4561 agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac
     4621 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgattgag
     4681 taaggcgatt gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatgtgctc
     4741 cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcaa
     4801 agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg
     4861 acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc
     4921 atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt
     4981 gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca
     5041 tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa
     5101 aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt
     5161 cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct
     5221 gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc
     5281 ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt
     5341 aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca
     5401 tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc atttccattg
     5461 ttgactcctg tatcactata ctacggtgaa aaaaaagaag gctgagtatg ccttctttta
     5521 tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag
     5581 gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac
     5641 acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag gattgcaggg
     5701 ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag
     5761 gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag
     5821 agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg
     5881 cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc
     5941 tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt
     6001 cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca
     6061 acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc
     6121 ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa
     6181 ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg
     6241 catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt
     6301 ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg
     6361 aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac
     6421 ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac
     6481 ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt
     6541 atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt
     6601 caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt
     6661 attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc
     6721 ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt
     6781 gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac
     6841 ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta
     6901 acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc
     6961 cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct
     7021 atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg
     7081 gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg
     7141 ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt
     7201 gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc
     7261 ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta
     7321 ttcactggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg
     7381 ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct
     7441 ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg
     7501 gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc
     7561 ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta
     7621 ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga
     7681 tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccga
     7741 gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca
     7801 cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga
     7861 aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag
     7921 tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact
     7981 acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca
     8041 ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa
     8101 acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat gaacagggtc
     8161 gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg
     8221 ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga
     8281 gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca
     8341 tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca
     8401 ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc
     8461 ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc
     8521 tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg
     8581 ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct ctgagcctgc
     8641 agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa gttcaggaat
     8701 acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact tctgagcgca
     8761 gccgtcgtct gtctccgcct gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg
     8821 gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct
     8881 gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg
     8941 ttgcagcgtc tcagggtgta gaactgggtg cagggattga aatcgttgat ccagaagtgg
     9001 ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa
     9061 ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg
     9121 atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc
     9181 cgctgcagct gatcaaaact gcaccgggct gctccctggt atcttccgtg ttcttcatgc
     9241 tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgctg
     9301 aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac
     9361 cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa
     9421 aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc
     9481 cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc
     9541 cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct
     9601 acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc
     9661 gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg
     9721 cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg cagctttgcg
     9781 ctgcggatat ctgaaccgga aataatcact atttccggtt ttttattctc ttaatctgca
     9841 ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatttcact
     9901 ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg
     9961 aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg
    10021 ctattttaac cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag
    10081 tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc
    10141 gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg
    10201 gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg attaacctca ggatcgaaat
    10261 acgggacagc tgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg
    10321 tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga
    10381 atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg
    10441 gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg
    10501 gctatgacag tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca
    10561 gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc
    10621 tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct
    10681 ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt
    10741 ccgaccgctt ctttcgtgaa aagcaggctg atgttgaaca acgtccgttt acctttggtg
    10801 actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg
    10861 ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg
    10921 gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct
    10981 ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga
    11041 ttttgctgct ggtcggtaat ggtgaagcag gtgatgccag cgtgttaaat accatcctca
    11101 acagcattgc caatgccatt agcggtctgg acaacgctgt cgcggcctgg tttatgttgc
    11161 tcttccaggc ggtatttaat ttctttgtga catccggttc tggtcaggcg gcgttaacca
    11221 tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg
    11281 cattccagtt tggtgatggc ttcagccaca tcatttaccc aacctcagct tcgttaatgg
    11341 cgacgctcgg tgtttgccgg gtggacttcc gtaactggct gaaggtgggc gcgacactgc
    11401 ttggactgct gtttattatg tccagcgtcg tggtgatcgg cgctcagttg atgggctacc
    11461 actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta
    11521 cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc gagtctggag
    11581 tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt
    11641 catcttcctg tagggcgaag ggaccgtgag agacgcagct gaacaatgcg ccccagacac
    11701 ggcaattttt atcttcgaaa tagaactgcc cgtgctcagc aaaggggaca ccggcaatgc
    11761 ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctggacca
    11821 caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct
    11881 ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac
    11941 actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa
    12001 tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt
    12061 ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg
    12121 caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat
    12181 aactcgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct
    12241 ccccccgctg ttctgccact ggcagatggg tatggccgta caccagcaca tcgttctggt
    12301 ttaaagcagg cagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt
    12361 tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca
    12421 cttcgctgtc gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt
    12481 cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca
    12541 cgtcgccaag gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt
    12601 ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct
    12661 cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc
    12721 gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt cgcataatcc
    12781 accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga
    12841 tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtac taacaaatgt
    12901 tctgccatcg caaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt
    12961 ttcgcccccg caaagacaac atccgtcgga cgctcttcgc ggatgggcat cagatcgctg
    13021 cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata
    13081 cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca
    13141 ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc
    13201 cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg
    13261 cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa gtgggcatct
    13321 gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat
    13381 caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct
    13441 ttctatactt gtggtgttca gatgattaaa caaccggagc tgcaatgatc gatctctatt
    13501 tcgccccgac acccaatggt cacaaaatta cgctgtttct cgaagaagca gagcttgatt
    13561 atcgcttgat taaggtagac ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca
    13621 tttcgcccaa caacaaaatt ccggcaattg ttgatcattc tcctgccgat ggcggcgaac
    13681 cgttaagcct ctttgagtct ggtgccattt tgttgtatct ggctgagaaa acaggactct
    13741 ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc tggcaggtag
    13801 gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca
    13861 ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctttaccat gtactgaaca
    13921 agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct
    13981 gctggccgtg ggttaatgcc tggactcgcc agcgaattga cctcgcaatg tatccggcag
    14041 tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag
    14101 cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca
    14161 tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa
    14221 gaaccttcgt ttgcgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga
    14281 tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat
    14341 caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa
    14401 ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca
    14461 ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc
    14521 ggtatccatg accttaagct ggcagcgtta accgccatat cgggaggctg catgaacata
    14581 gtgatcaccg gagggacggg attaattggt cgccatttga ttccgcgttt gctggagctg
    14641 ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag ccagttccgt tctcggccct
    14701 cgggtgacgc tatggcaagg gctagccgat caaagcaacc tcaacggcgt tgatgcggta
    14761 atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacgagca aaaagagcgt
    14821 ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac
    14881 acgccgccgt cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa
    14941 gtggtggtta ccgaagagga accgccgcat aacgaattta ctcataaact ctgcgcccgc
    15001 tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc
    15061 ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa tgctgccacc gtttcgtctt
    15121 ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca tattgatgat
    15181 atggtcaacg gcattctctg gctgctggat aacgacctgc gcgggccatt taatatggtt
    15241 tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc
    15301 ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcaata
    15361 ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt
    15421 cgctggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa
    15481 acatccgcca gttaactccc ggtgttacag gattagtggc tttgcgcgat aagatcgtct
    15541 ggtgaaagtc gggtcaccat cataactaac tctctgtcta aacctctatc cagcatctcc
    15601 tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg
    15661 tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata
    15721 aatgcacgga aacgctgggc atcccctgtt tgtaatacgt aattaaacag ggcttttagc
    15781 tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac
    15841 agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtgttgc
    15901 ataatctcgt catccggcac cacggtaata tccaccaacg gaaacgctga tgaatatatt
    15961 ttgcgggcta tagcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga
    16021 cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca
    16081 tcaagatggt tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct
    16141 tccggcttac tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc
    16201 acagaccaca agaggtcgga ataatattgc cgcaggtcgt catcaataaa actgtttggt
    16261 tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca
    16321 ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc
    16381 gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac
    16441 aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag
    16501 aagtattttt agcggactgc aacttttaca tttcagcgga agctgactca caaattcccc
    16561 atgaaaatac tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag
    16621 gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc
    16681 ttctattttt ccctgatgga ggaaaatgac gtgagtagaa acatggcgag caaagcccat
    16741 ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca taatacgtaa
    16801 cacttcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc
    16861 cggttccatc gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg
    16921 caccggatat ttcccctgcg cacgttcgtc tatcccgact ttcgccagat acttcaccgc
    16981 ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt
    17041 ttccagcacc gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt
    17101 gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccatctt tgtcgcgcac
    17161 cagattgatc gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt
    17221 aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc
    17281 tccggcattc gcttgcagtg agaccccttt cagcacttca tgttcgccgt agcgtttgtg
    17341 caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt
    17401 tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga
    17461 tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt
    17521 taatatcgcg ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag
    17581 agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag
    17641 gcaaaataat gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa
    17701 tttccccatg cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg
    17761 tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga
    17821 attcggttcc cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa
    17881 catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt
    17941 tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg
    18001 acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca
    18061 gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac
    18121 ggagtagcgg cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta
    18181 aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg
    18241 ggtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat
    18301 cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat
    18361 ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt
    18421 cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc
    18481 gagagtaata ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc
    18541 cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc
    18601 cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg
    18661 cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat
    18721 agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat
    18781 gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa
    18841 gggagcgggc tttctttcac cgatggcgat taaccgccat aaacatcaaa atcgaagtac
    18901 tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt
    18961 gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt
    19021 ttttcatctt taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg
    19081 aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg
    19141 tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa
    19201 tgttcattac cgaacgtctc ctgggtggtg ccctgcaata cccctacccg tttgcctttc
    19261 agcgactcga ctgtcggctg aatgtcggaa tttttcgcca ccaccaaacg agaatcggca
    19321 gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat
    19381 gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt
    19441 tcgacaaacg tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg
    19501 aagccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta
    19561 ccgatgcgga tgttttgcgg aatcgcagca aacgccgcag ttgcgctgga gaaggccaga
    19621 accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata
    19681 gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat
    19741 atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc
    19801 accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt
    19861 cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc
    19921 gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc
    19981 atcatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga
    20041 tgagccagca aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc
    20101 aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc
    20161 ctggttggca taggccacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc
    20221 ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg
    20281 ctgaatcggt gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga
    20341 gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat
    20401 ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt
    20461 cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc
    20521 tttagcatct ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc
    20581 cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag
    20641 aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg
    20701 ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat
    20761 tcaacgttaa atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt
    20821 atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat
    20881 cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg
    20941 caaagagatc ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat
    21001 ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca
    21061 ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt
    21121 cacgcacgca gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca
    21181 gtaagccatc agtatagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa
    21241 tcaccatccc cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat
    21301 cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata
    21361 aggtctggcg cgccgcctgg ctcatcacca gatgcgtttt gatatctgtg acatcgcgca
    21421 gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga
    21481 gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg
    21541 gagttgcaag tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc
    21601 atttcgagat tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac
    21661 gtatcgagga aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac
    21721 accgagcatt caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg
    21781 ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg
    21841 cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag
    21901 cgaatttccg gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc
    21961 tcgataatct gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt
    22021 ttatcgcgga actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc
    22081 tgctggcccg gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg
    22141 tacggtttgc ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc
    22201 gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc
    22261 gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga
    22321 gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg
    22381 gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg
    22441 aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg
    22501 ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa
    22561 gcaaccatac cgtggccgat aatcatcgcc acacaggcat atgcgccgcg gattaagcgg
    22621 tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaagttg
    22681 tccagctcgc tagcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg
    22741 cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg
    22801 tgggcaagcg taatgccata cggagagtta acgtaaaacg gctgcgcttc agaggcgctg
    22861 gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc
    22921 atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag
    22981 ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt
    23041 aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac
    23101 atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt
    23161 gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaattg cgggatcagc
    23221 tgtgatttgc tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga
    23281 atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg
    23341 gtgcctgaca accccgtttt ctccaccaac tggcctatca cgaagttcac gatagcacca
    23401 acgatcaggg tagcgataaa cagtaccgcg atggcaattc catttcgaac cagttcgtct
    23461 tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca
    23521 caaccccatg tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga
    23581 gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga
    23641 ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta
    23701 gggatttcct tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac
    23761 tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg
    23821 acctacggtt ctaacactgc ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga
    23881 ctgtattaat tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac
    23941 tcacccagcg aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta
    24001 attttaccct gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc
    24061 ttaccgacaa tctcattcac tttatcggca tttttcagcg cacccagttg cacaacatag
    24121 gctttacccg ttggcgctgc tttttcttcc acgaccggct tcggttctgg cttcggcgca
    24181 ggtggtgctt caaccttcgg tttaggcggc tccaccggtt tcggcttcgg tggggcgacc
    24241 ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa tagtggtcgg atcgagcgac
    24301 ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc
    24361 aacgcctggg tggcggctgg catcatatca ggctcatcgc gatcgcccgc tttcggcacc
    24421 agcgggatag ccgcgaactc atcctgataa tgttttttct gcccgtccag cagccctgga
    24481 agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta
    24541 cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgacag tgtggaaaga
    24601 accacacacc agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg
    24661 cgcaacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc
    24721 accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc
    24781 cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca cgcgcccgtt
    24841 tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac
    24901 atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct
    24961 ggcaatcccg tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc
    25021 cagcgccgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc
    25081 gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg
    25141 acgttgtaac agtgcacctt tttcctgtgc cacatcagca atggtagaag gcatttccgg
    25201 ctcaccgaca attgccggtt tttcgctgcg gaagatgcct gctttctcgc gaccaatact
    25261 ttcgcgatct ggacccagcc agtcggtatg atccagcgca atactggtta ctaccgcgac
    25321 atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac
    25381 cacgtcaagt tgtgcctgct tgaacagcca caacgccgac agcgtaccgt actcgaaata
    25441 ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg tgtgggccga
    25501 ttccggcaat tcctggccct gcacacgtac gcgctcggta taacgcacca gatgcggtga
    25561 actgtagacg cccactttgt accctgccgc catcagaatc gactccagcg tacggcaggt
    25621 ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc
    25681 aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag
    25741 gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat
    25801 aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc
    25861 tgcccttatc agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc
    25921 ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc
    25981 cagtttcagg cgcatttccg gacgacggac gatcatgtcg atcgcgcctt tctcgatcag
    26041 gaattcactg cgctggaatc ctggcggcag tttttcgcga acggtctgtt cgataacacg
    26101 cggaccggca aagccgatta acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc
    26161 gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc
    26221 gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag
    26281 tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagcgggc agttatcttc
    26341 cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc
    26401 cataaaggcg aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat
    26461 caccaccagc gcatcttttt cgccagtttc tttctgcgca gatgccagac ggtctttata
    26521 cttcttggag tcacgaaact tcagcacatc tttcggctca agctcgctac ccagctccac
    26581 aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg
    26641 gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc
    26701 gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat
    26761 gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga
    26821 tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt
    26881 tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt
    26941 actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac gaataaattc gaaaacccca
    27001 ggcaatattg aaacaaaaat aatgccaaca atcatcagct taaggttgct ctgaatgaag
    27061 gggagcgtgc cgaagaaata acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg
    27121 atcacgttat aagcggcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggc
    27181 gcgaacgttc tgacgatcgg tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc
    27241 tcgtaaaact gatgagtctt atcaagataa ctgcgacgaa agattttcga atccgggtta
    27301 ctgaacagtt tgtcaccaaa taaccgtcca atggtatagt tcactgcatc accgacaata
    27361 gcggcaatca acataagcac caccatcata tggacattga gatcgttagt ttccagcgac
    27421 gccaatgcac cagcaacaaa cagcagcgaa tcaccaggca ggaatggcgt taccaccagc
    27481 cccgtttcgc agaacagaat caaaaacaga atggcataaa cccagacgcc gtactccgcg
    27541 accagctctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg
    27601 tccatatttg cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag
    27661 cctgaaatgt tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg
    27721 ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt
    27781 tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta tccagctctc
    27841 cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg
    27901 tacaaaggca ttcgctttga tatctaccac cacataagga ccgtgacgca tgacattaat
    27961 atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa
    28021 atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc
    28081 gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat
    28141 gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt
    28201 aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc
    28261 gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt gtacccctgc
    28321 gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg agagcgcctt
    28381 ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact
    28441 gccgtcgtac tcaatgccca gcgcaatttt ataaactggc agttgttgct ggtcggacat
    28501 tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca
    28561 aagcgaacgt tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta
    28621 cgcacgcagc caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat
    28681 tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg cgcttcttct
    28741 gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt
    28801 gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg
    28861 tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata
    28921 ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat
    28981 ttcgcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc
    29041 aggctggtaa cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc
    29101 agcagctggc tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc
    29161 agtacaaacg ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt
    29221 ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta
    29281 gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca
    29341 tcctgcacgg tgattgtttt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt
    29401 gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct
    29461 tcgcccacag cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt
    29521 tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt
    29581 aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg
    29641 cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca
    29701 gtttatcgaa ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca
    29761 catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcggg ccatgcagcg
    29821 taatgcgacc aaactctggc gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc
    29881 caataaactt gctataggct tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg
    29941 tataacctgc gatatgcggc gtgccgatat ccacttttgt cagcaactcc acgttaagtt
    30001 ccggttcgcc ttcccagaca tccagcacta cgcttaactt ctggccttca ttcaggcagg
    30061 tcagcaacgc agtattatcg acgactgcgc cacggcaggc gttaatcaga atcgctcccg
    30121 gcttcaggct acggatcagt ttttcatccg ccagatgcag cgttttgtac ggcccatctt
    30181 tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg aactaattca tccagcgagc
    30241 ggaaatcacc ctcatcccca cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc
    30301 ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca
    30361 ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag catcagtagt gaggagaaaa
    30421 catattccac caccgcaatc gcattacagc caggtgcagc ggaaaaacca attcccgcct
    30481 gcttcaacca tgcttcatcg acatggtcgg tccccgcagt ggcagtgcca acaaatttaa
    30541 tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat
    30601 ccgcgtctgc cagttgagca acggggattg gacgcccggg aaccgcggtc acctcaccca
    30661 aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg
    30721 tgttacctgt atgagacgag agttaaccgg acaagtgtgc cataatctcg cggccaggca
    30781 tacttgcgaa gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct
    30841 cctgggcaac caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta
    30901 tccagtcagc aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa
    30961 caacaaagag cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca
    31021 ccgctgcttt cgcgtcactt ccctgccgct gagcataatc tggcgcaacg tctgctggcc
    31081 gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg
    31141 gggaataatc gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg
    31201 aatcagctct caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg
    31261 gttattccgc aaccacagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac
    31321 aatgcgctaa aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag
    31381 atctggcaat cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa
    31441 ctgtttaacc atctggtgac ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg
    31501 ccgacgctgg aatcactaca gatggcgcta aaacaacctt tagatgccag tgaactggcg
    31561 gcgttatcgg catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct
    31621 gcccaggccg aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg
    31681 cgtgatatgc aaccgctgct taatcctttt ccaccgatga tggacacgtt gcaaaatatg
    31741 gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg
    31801 gttcgttagc cccgacgaaa tgacagtatc gtgacaataa ttcccagcac cgcagagatc
    31861 gccccggcaa gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc
    31921 ccggagacgc cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga
    31981 acttgtgagg ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat
    32041 ccggctccgg ttaacgccgc gcccgctaat gcgacccacg cacctggggc ttgccagagc
    32101 agcaacaagc ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa
    32161 cggtccggca tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta
    32221 agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg
    32281 ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca gataaacccg
    32341 acaacgctcc acagcgatgg acgttctccc gccagggccg gtactttgcg cactgtgccg
    32401 ttacaggccc acgccagtaa gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg
    32461 taatggctat gaatcaacag gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc
    32521 attccattcc atgacatcac tttgccagag tgttttggcc ctacgatgcc taacccccag
    32581 gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg cccgatgacc
    32641 aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg
    32701 ccagccagac cacaagctaa catcccctga agcgccgaac gttttgcacc atattgatcg
    32761 gccagtcgcc cggcatagcc acgcgtcagc accgtagcca gaaactgaat cccgacggca
    32821 atcccgacca tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc
    32881 aacggcagcc ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa
    32941 aaattggcag aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg
    33001 cagcgcaagg cgaggagtat ccccgtctca tctctctggt ttcagggtta cggtgcgttg
    33061 gcaggattta acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg
    33121 aacaggcggc gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag
    33181 tgccggaggc cgcattagcg tgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct
    33241 actccgacta ctccggccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc
    33301 gttggtgccg ccgaagccga agctgttaga cataacggtg gtcagttcgc gatcggtcgt
    33361 ttcggtcacg atgttcagac ctgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg
    33421 ggcgataaag ccgtgttcca gcatcagcag agagtagata gcttcctgta cgccagcagc
    33481 gcccagagag tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa
    33541 cacttcacgg atagctgcca gttctttcac gtcgccaacc ggagtcgaag taccatgaga
    33601 gttcaggtaa tcgattgggg tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc
    33661 gccttcgcca gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat
    33721 ttcagcatag atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat
    33781 accgccgccg ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt
    33841 ttccggggtg tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat
    33901 ttcccagcac agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat
    33961 ctgctctact gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta
    34021 gttaacgcca tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc
    34081 tttggtgacc acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc
    34141 gccgaacacc tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg
    34201 gttattctgg taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag
    34261 gaatgcataa atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt
    34321 ggtatccagt tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg
    34381 agagaaagtg atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt
    34441 accgatgctg gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg
    34501 taagtcgcac atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag
    34561 tccgatcagc catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc
    34621 caccgcctgt aagacgagta acttacgtga aacactactc catacaacct gccaacctcg
    34681 aatttaatgc tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg
    34741 ataacgggct ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct
    34801 ttcctgagca tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa
    34861 acttcctgac gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat
    34921 tacaacgctt acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct
    34981 tagcgcatca acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc
    35041 caatgccctt gcccggttgc catcgtttat tgctcgatga aggccgcgtg acgctggatt
    35101 tatggtttgg cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag
    35161 tagatgcctg gtttctggac ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa
    35221 atctgtttaa cgccatggca aggttggcgc gtccgggcgg cacgctggcg acatttacgt
    35281 ctgccggttt tgtccgccgc ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg
    35341 gctttgggcg caaacgggaa atgctttgcg gggtgatgga acagacatta ccgctcccct
    35401 gctccgcgcc gtggtttaac cgcacgggca gcagcaaacg ggaagcggcg attatcggcg
    35461 gtggtattgc cagcgcgttg ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc
    35521 tttattgcgc ggatgaggcc cccgcactgg gtgcttccgg caatcgccag ggggcgctgt
    35581 atccgttatt aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt
    35641 ttgctcgtcg gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca
    35701 cgcagttagg ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt
    35761 tacccgcaga actggctgta ggcgttgagg caaatgcggt tgaacaaatt acgggcgttg
    35821 cgacaaattg cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga
    35881 cccgtaatgt gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt
    35941 tacagaattt atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag
    36001 caacacacag cgtagtggta ctggcgaacg ggcatcaaat cagccgattc agccaaacgt
    36061 cgactctccc ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac
    36121 tggcagagct gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga
    36181 atcaacatca ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg
    36241 aggacgatca gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg
    36301 caaaagaggt tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg
    36361 atcatctgcc aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt
    36421 cgttggcgga gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt
    36481 ttgcggcttt aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg
    36541 cgcagatgag cgacgaaccg attccgatgg atgccagtac gctggcggcg ttaaacccga
    36601 atcggttatg ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc
    36661 tcgcagtgtt tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc
    36721 ctgataagac gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc
    36781 tgtgacgcct gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc
    36841 gacaattcac cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc
    36901 tgagcggagt ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc
    36961 aaatacccac tggcaaacaa ctcatcatca ctggcatggt caaccatacc gtcgattaat
    37021 gccagaatgc gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac
    37081 cggcttcagt tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga
    37141 taataatttt gtagtgcttt aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg
    37201 taatccatca cctgcgcatg tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg
    37261 ccttccagat tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag
    37321 tcgaacgcct gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt
    37381 ccgtccgggc aataccagta gccgaaatca accagctcac ggcgcgcttt cccagcaata
    37441 caccagtgcg aaatctcatg aattgcgctg gcataaaagc catgagcaaa gacgattcgg
    37501 ttatacggta cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga
    37561 cgggtattaa attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg
    37621 tgtgtactgt tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcaa
    37681 aaagtaattt ggcactcatc accgctgaga caataacaat catcgggcgg atcagctttt
    37741 gtcctttgct caacaccagt cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca
    37801 tgacaaagcc cgtcgcccaa atcactttgc caccgagaat aaacagcagt aaaccgccga
    37861 tgtttgacgt tgcgttgagt aatttggcgt gcgccgtggc tttggcgagg ttgaatccgc
    37921 acagcgtaac aaaggccagt gcgtaaaacg atccggctgc cggaccaaag aatccgtcat
    37981 aaaaaccgac gcagccaccg gcaattaacg cgaagggtaa accgtacatc cgacgctggc
    38041 gatcttcttc acccagcttt ggcatcagca agaaatagag gccgatacaa attaccagaa
    38101 tgggcaaaat ctgccgtaag acatcagcct gaacgtattg caccagcagt gcgccgctca
    38161 ttgagccgac aaaggtcatg gcgatattga gtttctgatc gcttaaacta accactttgc
    38221 ggcgaataaa gtagatagta gcggaaatag agccgccgca ggcttgcagt ttattggttg
    38281 ccagcgcatt agcgggagac atccccgctg ccatcaatgc cggaatggtg agtaacccac
    38341 cgccaccggc aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca
    38401 gcaacagcgg ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc
    38461 cagtagcgcc tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt
    38521 tcccggtttt ggaggttcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg
    38581 cggtaaaggt tgatcttcac actccagact atcggcagga caacgtaatc gtacatgcat
    38641 atgcgcacga tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc
    38701 atctaggcaa agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtttttgtc
    38761 ctgggcggcg agtttgatca agctgaaaat ttctggcttc cacagcgagg agacaacgtg
    38821 tttaccgtcg cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca
    38881 gcgagttttc ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg
    38941 accgccgttg aaacgcccac cagcaggcat ccccatatcg ccaatcagca ccgtacccat
    39001 gcccagattg ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc
    39061 gaaatagcga cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcagcgt
    39121 gtcagcgccg acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac
    39181 aggttgggtt attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc
    39241 cagcagcgca atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc
    39301 ggcattttgc gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc
    39361 tgcgatcggc actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc
    39421 aacttcttcg ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt
    39481 cagcgccata tgggcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc
    39541 atggttgctc tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc
    39601 cactacgtca aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc
    39661 atgggcgatg tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc
    39721 actggcaaca acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat
    39781 cagttcatct aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac
    39841 cagcgaccag tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat
    39901 ttcaatacca aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat
    39961 ggcggtttcg cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg
    40021 ttcgtaggtg taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg
    40081 agagcgctgg tcagtgtttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc
    40141 aaaaacaccg gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga
    40201 tgtcccaggg cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc
    40261 tggcggaaca ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt
    40321 tacgcgaaag agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt
    40381 gcgtgtttac ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt
    40441 tggtgagcat aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat
    40501 ccggatattg ttccataaga tgtaccatgc tgttgccgac ttcacaaatc agcacaccat
    40561 catcagcaag gtaatctgcc gcgttaccga gaatgcgacg cgtcagtttc aggccgtcgg
    40621 tgccagatgc caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat
    40681 cttccgcgtc gacatacggc gggttagtga caatcagatc gtattgcact ttcggcaaat
    40741 cacggaacag atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt
    40801 tctgttcggc aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg
    40861 ggaaggcata agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat
    40921 gctgcggttg cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggtg
    40981 agcgcggcac cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt
    41041 tggtcaggta agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac
    41101 ggtgtttttc gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca
    41161 ggtagagcga tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac
    41221 cgtaccagat atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg
    41281 tttgcagctc atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat
    41341 gatcgcaata atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc
    41401 gcggtgagtg aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg
    41461 taggaaaatt agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga
    41521 ccaggctctg tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt
    41581 ccaccgaccg cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca
    41641 ggctgatgcc agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc
    41701 ggtgaaatat gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga
    41761 ttattcaccg gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga
    41821 actgggcgcg ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca
    41881 tgggcacggg aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca
    41941 tgtgatggca tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt
    42001 gattgaagtg gaagagtggt tgccgccgga gttgccctga ggagttgagc gtgttgtgcc
    42061 aggagcgcgg aatggcaggg gcgaatgacc ctgccaagtg agaaaaggtg cgctaataca
    42121 tattcagtat gtaagtgaat cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca
    42181 cgcgtagctt catatcatgc agttgcctgg acgtgatcgg ttgcggctct ggcgtcaccg
    42241 tcactttggc attatcacag ccggaagtcg agaaggtcat ctgctgtgcg acaaaacggc
    42301 agtaggctcc acgctcgtca aaattactgc catcacacag tttgccgttt tccatcagga
    42361 aagtgggcag tgtttgctca ataacaccac ctgttgactg caacctgacg actttgttat
    42421 gtacaacata attttcattc caggaaacat catacggtgc cagggactct gtcgggcagt
    42481 cagagctaac ttttgttggt cctataaaaa ttccggcccc gaaacgctga tccatagtac
    42541 tggcgctttc aacataagac gacacaatcc agggacaacc gtgtaaatct ccatcgtaat
    42601 aaacgccaag tactaattca ttacatgacc aggccgttac tggagagaga gcatcatact
    42661 cttctaatcc ggggcaggta gaatccggta aggttgtatt attgcgctcc atcctgagtt
    42721 cagggccggg aggactcccg gtccaactaa ctaaaggcaa acttgctgcc gcagttgaat
    42781 tagattcata cgtgccatat tcaactgatt gaggggtcag gactgaaaga tgaaaacgat
    42841 actttgcaac aacgctggtc gtccccgcgg gtacatagag tgtggcgatc tttgtcgcag
    42901 cctgtatccc agaagcgcac agagccagca ccaataaaca tataacatga cgtattttat
    42961 gcattttata tatactcaaa cgtagctgtc aggctggcgg tgaaatcacc gatggttaca
    43021 tcacgaccgc ttttcgcctg gatccaggcc cggaaattaa tatcattgtt gccgttggta
    43081 agcgcaaaag tcgccccggt ggtgttgtta atatccaccc gcttgccgtc gagcgtttcc
    43141 atccctatcc ccacgccctg cgcggtggag gtgtcgtcga ctgccagaaa acccggctga
    43201 ccgctgtctt ccgttccggt gagggtgact ttgacgttgt atcccgccgg ccctttgcag
    43261 tctttcagct taaacacaac cggcagatgc gcggactgcc cggcgaccat caaatcccgg
    43321 ttgctgacgg tgggaaaatg aatttccgcc agattggcac catccaccac cagcgtgcag
    43381 gatttactca gcaggttgcc gtaaaaatgc aggttgttat cgactgcccg tgcccccgtg
    43441 ctggcggtca gcagcaggcc tgctaacagc gcataaccgg gtgaataaat gtgtgctgtt
    43501 ttcattggta atccacccgt aaggttgcgt aagcagaaaa attcgtgtcc gtcagcaccg
    43561 catcagccgc tttgaccggt accgcctcca gctttggctt ctggctaaaa tcggtggcgt
    43621 taatggaaag cggctcgttg agcttaaaac gctgaccgtc atgctgaagc tcaattccga
    43681 agcccggtac atccgtctca atagcagagt cgttgtagga ggtggcagtc cccgtccagg
    43741 agagtgtcat attccactcg ggatcgcgga catccgagtc gcagctcagt tcgtagtcca
    43801 ctttctgacg accataattg ccatcaatgc tgtcgataat aagatcgcga aattgcactt
    43861 cgatggtttg cccgccgctg atgctacagg cgggcggcga catcagcgta ccgtggaatg
    43921 tgatgtcatc ttccgccgcc agcacagccc cgcaaccagc gcacagtaaa aggagcagga
    43981 attttttcat tggtaatcca ccaccatggt catcgtggcg ttaaactccg acggcgtcag
    44041 ttgcaccccg ctctgtttta ccggcaccgc tttcagctga ggctgagtgt tgacattaaa
    44101 aggcgtccag ttattttcac caactgcaaa cagggagttg tcggcggcgt tttctatccg
    44161 cacccccagg ccggcgatat ccgtgctgag aaccgtttct ccgttgatca cggcggtatt
    44221 gcctttgagc tgcatccgta agtcatcgct gactttatta ccgcagttga gggtgtattt
    44281 cgcatcctgt tggtaattgg taccatcgat cctgttgatt atcacattgc caaatttcac
    44341 agcgcttccc ttcacggagc aaggcggcgg tccatcgacc aggattcgca gcgtcagatc
    44401 cacctgtttg atatcactgc cagcaacagc caaaccgctg ataccccatc cggccagcac
    44461 cagcaaccct gcgcccgcta caaacttgtg actcataagt ccctcccggc atcagccttt
    44521 gcggctctgc tcttcatcca cctggcagct gttgccggtg catataaagt acagcggcat
    44581 ccgcgcgccg tagtcgttga tgtaagtcag caccggcacg ctgtccatct tcgcattcag
    44641 cggtgcggag gttttcggct ccatcatcag cggtgtgaat cccgccggcg ggttgccgcc
    44701 ttttttagcg ctggcgttgc tgatgatcac gtagtacggc gtcgggttat tgacggtgaa
    44761 gtcctggccg ttacggatga gcgtcacctt atgctgccac ggacttttca tgctggtgtt
    44821 ctccagcgcc ttcggtcgcc agaacagttt gatacgcgtc tgcagcgcaa tctgcagtgt
    44881 gttcggttta tttgatttcg gcggaatttc gcgcacgttg aaatagaaca cgctctcgcg
    44941 gtcggccggc agtttgttga tatccggcag cccctgcacc ttcacctggc cgttcatcat
    45001 cgcatcgatg cgctgcactg gcggaagcac cgcaagcggc gagctgattt tgttgccctt
    45061 tgcatcctct atccagctct gcgccagata aggcagtgtg ggatcgttgt tgcgcagcgt
    45121 gacgctgatg gacttgtcgc tttcgttaaa caccagccgt gtcctgtcgg gcgtcaccga
    45181 agcgtggctg gagaaactga ccgctgtcgc cagcagcgcc agtgccggga caacgatttt
    45241 tgcagtgtta gtcagattaa acatggatca ttccttaatc acgttactgg tttactgaaa
    45301 ttgaagtcgg aggttccgtt ggcgtcaccc gctgtgtctg ttcctgaatc gccggtttga
    45361 ccgccggcga agtgataggt gctacattgc ctttatgctg gcatggcagt aacaagccgt
    45421 taaacagatc ggcaggcagc gggtccggca ggttgatatc gcaatgcgca acaccactcc
    45481 agaacacact catgtgttcg ccaggtttca ccccagccag ataaacattg ccgtcatcat
    45541 cgacaaggcc cactgtctgc tcgttatcat tttttacttc tgcgccaaac ggaggatggc
    45601 tgccgtcgct caggcgcagc accgccatcg ctttttgtcc gctaatgaca gcaaatttac
    45661 ggtagccaat ggcaccttca gttagcgtgg cttgcaccac cgactgggtt gcttcagcgt
    45721 tttcaggcaa tctgttgagg tcgatatacg cctgattgcg gtaatagtta ttgacgtcag
    45781 aaacgacggc tttaccaaac atattggtat aaacagcagc cccgttacct tccaccggaa
    45841 catcggcaac gccatcggca tcaatcaaca atcgtgtccc gcccatattc tgggtacggt
    45901 gaagtgcgcc accgtgggca gtaagcgttg cgccgccctg taacgacaag cccgcagagg
    45961 tgtattgccc ttcatggtag ttcgcactga ggtcaacctg cgccagcgaa ccatcatggc
    46021 tgtaatagcc gtcaacgctg gtgtgtttgt cactggtgcc gacgttcaac tgatagtgag
    46081 tcgcgtcatc gacacggctg aaataaccga cctgactgct gtccgtccca ctgccatagt
    46141 taccgttata gctaacggta ctgttgtcgc cccacggcat actgagcgaa atgtacatgc
    46201 ctttgtcggc ctggttgtca tactcgtaac ggtagccagt catcgagatg ctgacattac
    46261 gaatactgcc catattgaaa tagtgcgaga gcatgatgtt gtagtttgtc tgctcctcgc
    46321 gatcccagta ggtatggcgg gtatagttga gataaaccga aacacccgca tcgcggaagt
    46381 tctggttata agtggcggtg tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt
    46441 cactggcatc cagatactcg ctcatggtca taaagttctc ttccgagaag cgatatccag
    46501 caaaagtaac gcggctgttg agctggtcaa agtctttgga ataactcaca cggaaggagt
    46561 taccgtccag cgaacctttg ccataagcgg tgtctttatc cagtttggtg tgcgagtgag
    46621 taacatcaaa cgcgaccgcg ccgaatgtag acaaatcgcg accgacgcca agcgccgcag
    46681 actgatagtt ttcatctccc agtgcgccgc catataacga ccagccgtta gcgatccccc
    46741 aggaagcttc agcaccagaa aaaaattcac cctcgacatg gtgtccccac tcttgcggac
    46801 ggcccatcat gatcttatag cgaacctgac ctgggcgagt gaggtatggc atcgaggcgg
    46861 tgctgatgtc atattcctgc acctggccgt tctgttcttc aatgcgaata tgcaacgtac
    46921 cggagacaga atcaccaaga tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt
    46981 aaatcacacg ccccatctgg ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa
    47041 tgtctggcgc gtagccgcgc agattgggag gcaacatttg atcgtcagta ctgacactgc
    47101 caccaacata gttaaagcca tcaaaaatat cggatctgag gtaatcctcg cccagcgcca
    47161 gtttggcttt taatgatggt aacgcccgcc aggcatagta gcgactccac tcccattttt
    47221 tttgagtttc atcgccgctg aattcatcgt catcatcatt actgcgagta tgttgatagt
    47281 tagtctgcca gtcagcacgc atacgccacg gccccaggtt aaccccgacc gtcccgttgc
    47341 cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc
    47401 tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga tcccagtcgg
    47461 gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat
    47521 cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc
    47581 attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca
    47641 gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt
    47701 ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc
    47761 cctggctgga aaaacgcttc agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg
    47821 aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg
    47881 caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta
    47941 tccgttttta ttcttgttcg gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg
    48001 ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcgaaa ttacccagat
    48061 ctggcgcatc agctgcgccg accagccagg ctttaaagtt cagtgtctgc ttcgctttgc
    48121 ctttattggt agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag
    48181 aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg
    48241 tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag
    48301 tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg aaacttttcg
    48361 gcgtggtggt gccgttattt ttcagttttt cggcaccaat atcacccata tcaacttcca
    48421 gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg taaaaacgtg
    48481 cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaaaacgccc atcaccattg
    48541 ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact ataaataatt
    48601 aaaaatataa acattacaat acattgattc agtcaatacc caatgtttat acccagccat
    48661 tgaatacggc atgcgatcca aaaagaacag gtcgtagaaa attgtattct ttggtgtggg
    48721 gccgcatagt gatgttttca cccccctgat gcaatttaat tttactaaag agtaaatgt
//
LOCUS       DAZUFB010000026        47892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZUFB010000026 DAZUFB010000000
VERSION     DAZUFB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.026"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..1138
                     /gene="phoE"
                     /locus_tag="ACHY3M_001712"
     CDS             83..1138
                     /gene="phoE"
                     /locus_tag="ACHY3M_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS4316645.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1177..1578)
                     /gene="crl"
                     /locus_tag="ACHY3M_001713"
     CDS             complement(1177..1578)
                     /gene="crl"
                     /locus_tag="ACHY3M_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS4316646.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1636..2880)
                     /gene="frsA"
                     /locus_tag="ACHY3M_001714"
     CDS             complement(1636..2880)
                     /gene="frsA"
                     /locus_tag="ACHY3M_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS4316647.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2972..3430)
                     /gene="gpt"
                     /locus_tag="ACHY3M_001715"
     CDS             complement(2972..3430)
                     /gene="gpt"
                     /locus_tag="ACHY3M_001715"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4316648.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3691..5148
                     /gene="pepD"
                     /locus_tag="ACHY3M_001716"
     CDS             3691..5148
                     /gene="pepD"
                     /locus_tag="ACHY3M_001716"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS4316649.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5205..5819)
                     /gene="prfH"
                     /locus_tag="ACHY3M_001717"
     CDS             complement(5205..5819)
                     /gene="prfH"
                     /locus_tag="ACHY3M_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFS4316650.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5816..6955)
                     /locus_tag="ACHY3M_001718"
     CDS             complement(5816..6955)
                     /locus_tag="ACHY3M_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFS4316651.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7201..7653)
                     /gene="yafP"
                     /locus_tag="ACHY3M_001719"
     CDS             complement(7201..7653)
                     /gene="yafP"
                     /locus_tag="ACHY3M_001719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4316652.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7650..8705)
                     /gene="dinB"
                     /locus_tag="ACHY3M_001720"
     CDS             complement(7650..8705)
                     /gene="dinB"
                     /locus_tag="ACHY3M_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4316653.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8776..9699)
                     /gene="lafU"
                     /locus_tag="ACHY3M_001721"
     CDS             complement(8776..9699)
                     /gene="lafU"
                     /locus_tag="ACHY3M_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFS4316654.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9702..10565)
                     /gene="motA"
                     /locus_tag="ACHY3M_001722"
     CDS             complement(9702..10565)
                     /gene="motA"
                     /locus_tag="ACHY3M_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS4316655.1"
                     /translation="MQKILGLLVVFGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10578..11294)
                     /locus_tag="ACHY3M_001723"
     CDS             complement(10578..11294)
                     /locus_tag="ACHY3M_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFS4316656.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11314..11781)
                     /locus_tag="ACHY3M_001724"
     CDS             complement(11314..11781)
                     /locus_tag="ACHY3M_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFS4316657.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11789..12850)
                     /locus_tag="ACHY3M_001725"
     CDS             complement(11789..12850)
                     /locus_tag="ACHY3M_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS4316658.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTQELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12847..13158)
                     /locus_tag="ACHY3M_001726"
     CDS             complement(12847..13158)
                     /locus_tag="ACHY3M_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFS4316659.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13163..13555)
                     /gene="fliS"
                     /locus_tag="ACHY3M_001727"
     CDS             complement(13163..13555)
                     /gene="fliS"
                     /locus_tag="ACHY3M_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS4316660.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13578..14894)
                     /gene="fliD"
                     /locus_tag="ACHY3M_001728"
     CDS             complement(13578..14894)
                     /gene="fliD"
                     /locus_tag="ACHY3M_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFS4316661.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15147..16061)
                     /gene="lafA"
                     /locus_tag="ACHY3M_001729"
     CDS             complement(15147..16061)
                     /gene="lafA"
                     /locus_tag="ACHY3M_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFS4316662.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            16547..17389
                     /locus_tag="ACHY3M_001730"
     CDS             16547..17389
                     /locus_tag="ACHY3M_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4316663.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(17444..18421)
                     /locus_tag="ACHY3M_001731"
     CDS             complement(17444..18421)
                     /locus_tag="ACHY3M_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFS4316664.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDQANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(18438..19367)
                     /gene="flgL"
                     /locus_tag="ACHY3M_001732"
     CDS             complement(18438..19367)
                     /gene="flgL"
                     /locus_tag="ACHY3M_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS4316665.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(19382..20758)
                     /gene="flgK"
                     /locus_tag="ACHY3M_001733"
                     /pseudo
     CDS             complement(19382..20758)
                     /gene="flgK"
                     /locus_tag="ACHY3M_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(20941..21240)
                     /locus_tag="ACHY3M_001734"
     CDS             complement(20941..21240)
                     /locus_tag="ACHY3M_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFS4316666.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(21240..22340)
                     /locus_tag="ACHY3M_001735"
     CDS             complement(21240..22340)
                     /locus_tag="ACHY3M_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS4316667.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(22355..23020)
                     /gene="flgH"
                     /locus_tag="ACHY3M_001736"
     CDS             complement(22355..23020)
                     /gene="flgH"
                     /locus_tag="ACHY3M_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS4316668.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(23103..23888)
                     /gene="flgG"
                     /locus_tag="ACHY3M_001737"
     CDS             complement(23103..23888)
                     /gene="flgG"
                     /locus_tag="ACHY3M_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS4316669.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTLEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23967..24704)
                     /locus_tag="ACHY3M_001738"
     CDS             complement(23967..24704)
                     /locus_tag="ACHY3M_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFS4316670.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24704..25906)
                     /gene="flgE"
                     /locus_tag="ACHY3M_001739"
     CDS             complement(24704..25906)
                     /gene="flgE"
                     /locus_tag="ACHY3M_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS4316671.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25930..26643)
                     /locus_tag="ACHY3M_001740"
     CDS             complement(25930..26643)
                     /locus_tag="ACHY3M_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFS4316672.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(26643..27074)
                     /gene="flgC"
                     /locus_tag="ACHY3M_001741"
     CDS             complement(26643..27074)
                     /gene="flgC"
                     /locus_tag="ACHY3M_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS4316673.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27077..27412)
                     /locus_tag="ACHY3M_001742"
     CDS             complement(27077..27412)
                     /locus_tag="ACHY3M_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFS4316674.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27494..28231
                     /gene="flgA"
                     /locus_tag="ACHY3M_001743"
     CDS             27494..28231
                     /gene="flgA"
                     /locus_tag="ACHY3M_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS4316675.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28312..28590
                     /gene="flgM"
                     /locus_tag="ACHY3M_001744"
     CDS             28312..28590
                     /gene="flgM"
                     /locus_tag="ACHY3M_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS4316676.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28603..29031
                     /gene="flgN"
                     /locus_tag="ACHY3M_001745"
     CDS             28603..29031
                     /gene="flgN"
                     /locus_tag="ACHY3M_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFS4316677.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29158..30087
                     /locus_tag="ACHY3M_001746"
     CDS             29158..30087
                     /locus_tag="ACHY3M_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFS4316678.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            30266..32710
                     /locus_tag="ACHY3M_001747"
     CDS             30266..32710
                     /locus_tag="ACHY3M_001747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4316679.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVATKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(32828..33223)
                     /locus_tag="ACHY3M_001748"
     CDS             complement(32828..33223)
                     /locus_tag="ACHY3M_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFS4316680.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(33226..33660)
                     /gene="fliJ"
                     /locus_tag="ACHY3M_001749"
     CDS             complement(33226..33660)
                     /gene="fliJ"
                     /locus_tag="ACHY3M_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS4316681.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(33663..35000)
                     /gene="fliI"
                     /locus_tag="ACHY3M_001750"
     CDS             complement(33663..35000)
                     /gene="fliI"
                     /locus_tag="ACHY3M_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS4316682.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDFLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(34993..35703)
                     /gene="fliH"
                     /locus_tag="ACHY3M_001751"
     CDS             complement(34993..35703)
                     /gene="fliH"
                     /locus_tag="ACHY3M_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS4316683.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(35706..36716)
                     /locus_tag="ACHY3M_001752"
     CDS             complement(35706..36716)
                     /locus_tag="ACHY3M_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS4316684.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNCFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(36694..38340)
                     /gene="fliF"
                     /locus_tag="ACHY3M_001753"
     CDS             complement(36694..38340)
                     /gene="fliF"
                     /locus_tag="ACHY3M_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS4316685.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSRQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     SGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(38345..38686)
                     /locus_tag="ACHY3M_001754"
     CDS             complement(38345..38686)
                     /locus_tag="ACHY3M_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS4316686.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(38701..39687)
                     /locus_tag="ACHY3M_001755"
     CDS             complement(38701..39687)
                     /locus_tag="ACHY3M_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFS4316687.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            40074..40925
                     /locus_tag="ACHY3M_001756"
     CDS             40074..40925
                     /locus_tag="ACHY3M_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFS4316688.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            40918..41289
                     /locus_tag="ACHY3M_001757"
     CDS             40918..41289
                     /locus_tag="ACHY3M_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFS4316689.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            41286..42038
                     /gene="fliP"
                     /locus_tag="ACHY3M_001758"
     CDS             41286..42038
                     /gene="fliP"
                     /locus_tag="ACHY3M_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS4316690.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            42041..42313
                     /locus_tag="ACHY3M_001759"
     CDS             42041..42313
                     /locus_tag="ACHY3M_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFS4316691.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            42315..43097
                     /gene="fliR"
                     /locus_tag="ACHY3M_001760"
     CDS             42315..43097
                     /gene="fliR"
                     /locus_tag="ACHY3M_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS4316692.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTMEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            43087..44226
                     /gene="flhB"
                     /locus_tag="ACHY3M_001761"
     CDS             43087..44226
                     /gene="flhB"
                     /locus_tag="ACHY3M_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFS4316693.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            44210..46303
                     /gene="flhA"
                     /locus_tag="ACHY3M_001762"
     CDS             44210..46303
                     /gene="flhA"
                     /locus_tag="ACHY3M_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS4316694.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            46408..46686
                     /locus_tag="ACHY3M_001763"
     CDS             46408..46686
                     /locus_tag="ACHY3M_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4316695.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            46679..47035
                     /locus_tag="ACHY3M_001764"
     CDS             46679..47035
                     /locus_tag="ACHY3M_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4316696.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(47092..47796)
                     /gene="yafL"
                     /locus_tag="ACHY3M_001765"
     CDS             complement(47092..47796)
                     /gene="yafL"
                     /locus_tag="ACHY3M_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4316697.1"
                     /translation="MLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQ
                     ASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHN
                     ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRR
                     ATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSQLAEPFWQ
                     EHFLGARRILTEETIL"
BASE COUNT        10455 a        13219 c        12714 g        11504 t
ORIGIN      
        1 caatctcagg ttaaaaactt tcctgttttc aacggggctc tcccgctgaa tattcgcgcg
       61 ttaattaaaa tcaggaatga aaatgaaaaa gagcactctg gcattagtgg tgatgggcat
      121 tgtggcatct gcatctgtac aggctgcaga aatatataat aaagacggta ataaactgga
      181 tgtctatggc aaagttaaag ccatgcatta tatgagtgat aacgccagta aagatggcga
      241 ccagagttat atccgttttg gttttaaagg tgaaacacaa attaacgatc aactgactgg
      301 ttatggtcgg tgggaagcgg agtttgccgg aaataaagcg gagagtgata ccgcacagca
      361 aaaaacgcgt ctcgcttttg ccgggttgaa atataaagat ttgggttctt tcgattatgg
      421 tcgtaacctg ggcgcgttgt atgacgtgga agcctggacc gatatgttcc cggaatttgg
      481 tggcgattcc tcggcgcaga ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac
      541 gtatcggaac accgacttct tcggcgttat cgatggcctg aacttaaccc tgcaatatca
      601 agggaaaaac gaaaaccgcg acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt
      661 gacatatgac tttggcggca gcgatttcgc cattagtggg gcctatacca actcagatcg
      721 caccaacgag cagaacctgc aaagccgtgg cacaggcaag cgtgcagaag catgggctac
      781 aggtctgaaa tacgatgcca ataatattta tctggcaact ttttattctg aaacacgcaa
      841 aatgacgcca ataactggcg gctttgccaa taagacacag aactttgaag cggtcgctca
      901 ataccagttt gactttggtc tgcgtccatc gctgggttat gtcttatcga aagggaaaga
      961 tattgaaggt atcggtgatg aagatctggt caattatatc gatgtcgggg ctacatatta
     1021 tttcaacaaa aatatgtcag cgtttgttga ttataaaatc aaccaactgg atagcgataa
     1081 caaattgaat attaataatg atgatattgt cgcggttggc atgacctatc agttttaatg
     1141 attattgccg gatgtgatgc atccggcaca tttcactcac gccgttaact tcaccggctc
     1201 gtcacgaaaa tcatccgccg gttccagctt cagattcagc gtcgtcagca gttcacgcag
     1261 cttctcgtga aactcacgca gggtgtgctc cagtcgttca accacttcag tgtcttttac
     1321 cggaacactc ttccagtcgc ctgctttatc gaacagacca aactggtaac tgtaggtaaa
     1381 acgggattcc tgcgcttcaa gctccatcca ccagccccag aactcacgca cttccggtgc
     1441 cggtttcacg ttgacgcata cagccagaca atcgaaaaag aatcgattat cttcgcactt
     1501 accttcacga atatacgggc ctagtgcggt aaattttttg atcaatctgc tcttcgggtg
     1561 tccactcggt aacgtcattg ctatctcctg ttgtgatgca actgttttac caaattggca
     1621 aaatttagca aatttttaac acaagcgttt ttcgatccag ccggtgattt cctgaagacc
     1681 tttgtcaaaa ttccgataca ccgggttaaa tgggatctct aataatttac cgtcagcaga
     1741 tgatgaggtg attaagcgtg agtcctcttc cgggctgaac ggatcgttct tccagtagcc
     1801 tgataacatt ggcgttgggc agcgacgccc cagcaatcct tgcactttta atgaatagcg
     1861 attcagctcc acgcgtaacg cgtcatcgga agcatcatgc atccccaaac gactcgccag
     1921 aacgtcaaga tacatttccg gcacctgttg ctggcactta aaatcactca acagggtatg
     1981 aactaccgga ccaagacagg caaccgcttt cagacgcggc gattcaagat atgccagacg
     2041 cacggcaacg ttagcgccga aacggaaacc aaaggccgcg acgcgagtgt gatccaccca
     2101 cggtacgtta ggcagcgcct ttaagacgtg ctgatgcaac aggctggagt cctgggtgag
     2161 cttccatttt gaagaaaagc ccaccgatgg catatcaata gtcagcatcg caatgccgcg
     2221 cggcgcaaaa taacgttcat acaggctgta atagtccgtc tgcatcgcat ccagaccacc
     2281 acacattaat actgtcggga acgggccatc gcctttcggc atatgcaaaa agccggtgat
     2341 gggcgcaccg ccgggtacgg taaactccat ctgccgcatc gtgcccggca gacgctgggc
     2401 ggcctcttca taggcgcggt ttgataaagc ctgcgcttgc tcggccaggt catctccttt
     2461 cagatgagga taggcggcaa tgttgtacaa cgtagccgca tgcagccagt gtcgaccact
     2521 gagttgcgga tcttcttccg cacaggcttt ttgttgccac accatcgcct gggtggccca
     2581 ctcataaatc cagttgccac cacggtagcc aatcacagta tcgtataaat catcgtcggt
     2641 acgttcagca tcgctcatca caatacgtgc ctggacgtcg aggatttcac gtgggtcaat
     2701 accgcgccag atccacatca gacggttaat catgcgatac cagtgaggga tggttttacc
     2761 atcaagagcc gactgcacag gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga
     2821 ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat
     2881 ggatgcagcc tccagaatac ttactggaaa ctattgtaac ctgcctgaag ttaaaaagaa
     2941 caacgcccgg cagtgccagg cgttgaaaag attagcgacc ggagattggc gggacgaata
     3001 cgacgcccat atcccacggc tgttcgatcc aggtatcttg cgggatatca acaacatagt
     3061 catcaaccag cggacgacca gccggttttg cgaagatggt gacaaagtgc gcttttggat
     3121 acatttcacg aatcgcaaca gcagtaccac cggtatccac caggtcatca ataacgatga
     3181 agccttcgcc atcgccttct gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat
     3241 cgtagctgga aatacaaacg gtatcgacat gacgaatacc cagttcacgc gccagtaacg
     3301 cacccggtac cagaccgcca cggcttacgg caataatgcc tttccattgt tcagaaggca
     3361 tcagtcggct tgcgagttta cgtgcatgga tctgcaacat gtcccaagtg acgatgtatt
     3421 tttcgctcat gtgaagtgtc ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga
     3481 aaataggttg cgcgagatta tagagatctg gcgcactaaa aaccagtatt tcacatgagt
     3541 ccgcgtcttt ttacgcactg cctctccctg acgcgggata aagtggtatt ctcaaacata
     3601 tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa ccctgtgacc tgcaatactg
     3661 ttttgcgggt gatcgacaag gagacttaac gtgtctgaac tgtctcaatt atctccacag
     3721 ccgctgtggg atatttttgc caaaatctgt tctattcctc acccgtccta tcatgaagag
     3781 caactcgctg aatacattgt tggttgggca aaagagaaag gtttccatgt cgaacgcgat
     3841 caggtaggta atatcctgat tcgtaaacct gctaccgcag gtatggaaaa tcgtaaaccg
     3901 gtcgtcttac aggcccacct cgatatggtg ccgcagaaaa ataacgacac cgtgcatgac
     3961 ttcacgaaag atcctatcca gccttatatt gatggcgaat gggttaaagc gcgcggcacc
     4021 acgctgggtg cagataacgg cattggtatg gcgtctgcgc tggcggttct ggctgacgaa
     4081 aacgtggttc acggcccgct ggaagtgctg ctgaccatga ccgaagaagc cggtatggac
     4141 ggtgcgttcg gcttacagag caactggttg caggctgata ttctgattaa caccgactcc
     4201 gaagaagaag gtgaaatcta catgggttgt gcggggggta tcgacttcac ctccaacctg
     4261 catttagatc gtgaagcggt tccagctggt tttgaaacct tcaagttaac cttaaaaggt
     4321 ctgaaaggcg gtcactccgg cggtgaaatc cacgttggcc tgggtaatgc caacaaacta
     4381 ctggtgcgct tcctggcggg tcatgcggaa gaactggatc tgcgccttat cgatttcaac
     4441 ggcggcacac tgcgtaacgc catcccgcgt gaagcctttg cgaccattgc tgtcgcagct
     4501 gataaagtcg acgtcctgaa atctctggtg aatacctatc aggagatcct gaaaaacgag
     4561 ctggcagaga aagagaaaaa tctggccttg ttgctggact ctgtagcgaa cgataaagct
     4621 gccctgattg cgaaatctcg cgataccttt attcgtctgc tgaacgccac cccgaacggt
     4681 gtgatccgca attccgacgt ggcaaaaggt gtggtcgaaa cctccctgaa cgtcggtgtg
     4741 gtgaccatga ctgacaataa cgtagaaatt cactgcctga tccgttcact gatcgacagc
     4801 ggtaaagact acgtggtgag catgctggat tcgctgggta aactagctgg cgcgaaaacc
     4861 gaagcgaaag gcgcatatcc tggctggcag ccggacgcta attctccggt gatgcatctg
     4921 gtacgtgaaa cctatcagcg tctgttcaac aaaacaccga acatccagat tatccacgcg
     4981 ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa tggacatggt ttctatcggg
     5041 ccaactatca ccggtccaca ctctccggat gagcaagttc acatcgaaag cgtaggtcat
     5101 tactggacgc tgctgactga actgctgaaa gaaattccgg cgaagtaatt atttgatttg
     5161 ctgccggatg gcgtttaatc gccttccggc agtttcatcc ttcattatcc ttcgataaaa
     5221 gccatccctg taaatgtccg tctcgggttg ccacgttcaa tctggtgatg gaacattcgc
     5281 cgcagcgatt tcagcaccgc gctattttcc tgttgctgct gctccagctt ccaggcaatc
     5341 agcaatcgtg ccagccgctt gttagcatgc tgactacgct ctgactgaac cttcacgcta
     5401 ataccggatg ccaaatgcgt ggcgcgtacc gccgagtcgg ttttattgac atgttgaccg
     5461 cccggccccg acgaacgcaa cgtctcataa cggattgcat ccgattgttc ctgctcatca
     5521 gcggtaaaac gcccaatgcc cagaaaccag tttttgcgcc catgatgagg ccgatacgga
     5581 ctcggacaaa tccactgaat agtgccacac cagctttcgc ttaatgccca tgcgttatcg
     5641 ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt agtggcccgt ttctgtttcc
     5701 agcaccgtta ccgcgacgtc ttgtcgggca gcttctttaa ttagcctgtc cagcgccttt
     5761 ttcactgcga gacaacattc ttccggcccc tgcgctgagg agagttgtag caagatcatc
     5821 cttttttccc tccactgttt ttgagcgtta gcaccggacg cagccgcgcg acaggaataa
     5881 ttaaaccggc ctgcaccaga cattgcacca cgctttcagc cgatttatag gcttgtggcg
     5941 cttcttcaaa gatgagttgt ttatcgcgac aaattacccg actgccaagt tcagtacgcg
     6001 agagttgcgt cgccgtgtat ttcgctgcca gacgcccttt acactcggtg cgcccccatt
     6061 tacgccctgc cccatgcgcc agcgagtgga gcgttttttc gttcgcaaca ggctgtacaa
     6121 tccaggagta atcaccacgt gaaccgggaa taatcaccag accgttgtca tccggcgtgg
     6181 cacctttacg atgcaaccag ccctgttgat cgccgatttg acacgcgcta acaaagttat
     6241 gcgccacatc cagaaccgga cttcccgtgg ccttaacttg ttgcattatg cgcaaagcga
     6301 tcatctggcg attaatacac gcaaacgcca gcgcatcgtc atgttccgca atataccgta
     6361 gcgcgtcgtc actgccttga ggcaaaccat gatgcgagaa cgaggcaata tgccgctgta
     6421 aaatagactg ccccaccccc cgcgagccgc tatgaaccaa cagttgcaga tgttgtgcat
     6481 ccagacctgc cagtgcaaac agttcagcgt tgatgatttg atcaacttgt tgcagttcag
     6541 cgaaatgatt gccgccaccg atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg
     6601 ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaga tctgataatc
     6661 gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc catagcgcca
     6721 taccgcagcc gatatcattg ccgaccagtg ccggataaaa acgaccaaca gagaagaacg
     6781 ctgcgccaat cggatagccg cgtccgggat gtaaatctgg catccccact acgcgctgca
     6841 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
     6901 catcagatgc gatggtaaat accgcatcgg ataagggacg aatataattg cccatatacc
     6961 attccgtaaa tagactcgtc atacttcaag ttgcatgtgt tgcgacttga attattgaga
     7021 gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa aagaattaat
     7081 cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac ctccttgcag
     7141 tttgtggaat aagaaaacgc gcacatacta ggcttgcgtt attttcattg caagctggat
     7201 ttaatgttgc tgtttataac gcatataaaa attaataaac cactctcccc gacattcaac
     7261 gcgttgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg cggttatgct
     7321 tgcgtccacc gtaagttcgg attcagactt aatcaaaggt tttaacaaag cgctggcaac
     7381 cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atatagtgtt caacgcaggt
     7441 aataaagccg accagttttg cattaatgac tgcaacccgc acttgtgatt tcgcgagttt
     7501 ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgtg gtgaataatg
     7561 ctgactggca gtcatcgtaa ccgctctaat gaaaatagcg catagttgct gaaaatcgcc
     7621 aggctgatag tctcttattt gtatgttatt cataatccca gcaccagttg tctttccatt
     7681 tgcgggtcaa gcaacgtcac atgcagcccc accagtcgca caccgcgccc gccgcggcgt
     7741 tcatcccagg ttttacgcgc ggtggcgatt aagtcagctt tattcagccg cggccagacg
     7801 tgctcctgag tggtttgctg aaaatcatca aactttaatt tcactccctg acgggcgatc
     7861 agtaaatcag gttttacctt tgccagacgg cgttcaagtt ccggatacag ccgctcgata
     7921 atcgcttcac attcagacca gtgatgaata tcttccgcca tcgtgcgttc cacgccgacg
     7981 gattttcgta accgttcgct gttaacgtcg cgctcgtcaa tcccctgact acgctcccac
     8041 aaaatgcggc caaatttgcc aaagcgttta agcagcatca ccagatcaca cttttgtaca
     8101 tcaccgcagg tccgcagccc catcgcttcc agttttgccg ccgagacttt gccgacgccg
     8161 gggatttttg ccagcggtaa ggtttgtaaa aatgccggaa cttctgccgg cgtaatcaca
     8221 aactgaccgt tgggtttatt catgtcggag gcgattttgg cgagaaactt tactggtgcc
     8281 acgcccgcag acgctgtcag ttgcagctcg ctgaaaatcg tctggcggat ttcctgggcg
     8341 atgagggtcg cagaaccgtg gcaatggacg ctatcggtga cgtcgagata agcctcatcc
     8401 agtgacaacg gttcaatacg cgaggtgtag cgcgagaaga tttcacggat atgatttgag
     8461 gcttctttgt aggcgtcaaa gcgccccgga agcaaggtga gatgcgggca taatttgagc
     8521 gccatccctg tcggcatagc gctacgtacg ccaaatttac gcgcgggata attggcggtg
     8581 ctgatcaccc cccgacgttc gcggctgccg ccaatagcaa tagggatatc gcgcagggcg
     8641 ggattgtcgc gcatctccac cgctgcgaaa aagcagtcca tatccacatg aatgatttta
     8701 cgcattactc acctctcaac actggtaaag tatacagtga tttcagggtt tgagaaatgc
     8761 gtaaagattc agcatttaac gcgttcgctg cgaaagcacc tgctgcttat ccagcttttg
     8821 caccagcggc tgcaccactt tatccccatg ctgaccaaag tattgataca gcgtatcgga
     8881 cgcacttttg gtcagcacca taatctcaat gcgccggttg cccgcgcttt gcggattttt
     8941 ggaatccagc agcatctggt ccgccattgc gcttacctgc atcactttat cttccggcat
     9001 tccggcctct tccagcaccc gacgagccga aagcgcgcgg tcacccgaaa ggttccagtt
     9061 gttgtagata ttgtttttgt aagccatcgc atcggtatgc ccggtaataa taattttgtt
     9121 atcgagcgag tcgaaaactg gcgcgagctc caccagcagc gttttaaaga acggcataat
     9181 ctgcgcgctg ccgcgttcga acatattgcg attctggtcg tctttaatca gtacgcgtaa
     9241 tccctgggga acaatctcca tctccagatt cgcttccata tgcgcatcac gggcaatggt
     9301 gttgatactg gttgccagct cgccaagctc ggtggcggat ttcttcttca gcagcgccgt
     9361 tttctcattc acatcacgcg cttttttctc cgtctcttcc ggcaccgcca cggtggcagg
     9421 ttttggcgga tgagacgggc tgattttatt taacggcgac agcccgccac cgttaaaaat
     9481 cgactgcccg tgcagcgcgg cgataatgct ttcacgctcg gatttactga cgctgttgac
     9541 aatccacagc gtcataaaca gcgccatcat cgccagggta aagtcggcga acgccacctt
     9601 ccacgccccg ccatgatgcc cggcatgatt ctttttgatc tgccgccgga cgatggtggt
     9661 ttttgcccca cgctcgcggc ggttgcccgt ctttctcatg cttattcttg ctccagcatc
     9721 gcattcaccc acgcgtcgag gttggcgaag gtcggtttgg acgccagatg cagcagctta
     9781 cgcccggcat ccaccgccag cagcgtcggt ttgccgcctg cctgcgccac cagcacagta
     9841 cgcacgcatt ccagcaaaga atgttctgcc cgcgcctgtt gttccatggc gttggcgagc
     9901 gggtccatca ggcagtagca gataaagacg cctaaaaacg tccccaccag cgctgccgcc
     9961 actttcaggc cgatcagggc aatagagccg tcaatcgact gcatggtgat gatgatcccc
    10021 agcactgctg cacaaatacc aaatcccggc atcgcttcgg cggtacgttg cagcgagcgc
    10081 gacggcgtga gcagcgactc ttccgcagtg tccagttcct gatcgagaat gccttccagt
    10141 tcatgggcgt cgattttacc catcgccatc aggcgaaagt tatcggcaat aaaggtcacc
    10201 agccgcttct gggttaacac cagcgggtat ttctggaaaa tggtgctctc ttccggttgc
    10261 tcaatatgtt gatccagcat gcgcagacca ccgttttgca ccatttcgag caactcatac
    10321 aggcacatta atagctgacg ctgaaactcc ggcccgagct gttttttact gatcacacct
    10381 ttaatttgat gggcaatctc tttcagcaca tgtttcgggt taccaataat catcgccccg
    10441 aaaccggcac ccaaaataat gatcatctcc gccggttgcc agatagccac taattgcccg
    10501 cccgcttcaa aataaccgcc aaatacgcag ccgaacacca ccagtaaacc taatattttt
    10561 tgcatgattt cttcacttta attcggatag acaaaatcac gaattttctg cgcgatctgt
    10621 ttattcagtt gacaaattcg ggcctcggtt agccctaata ccagcgcaat ctctttgaga
    10681 ttcatctcat gctggtaata catggagagg atcagttgct ctttttcgct taattgtgaa
    10741 agcgcctgtt gcagcatttg ctgagtgaca aactgatctt ccagctcccg gccaacaagc
    10801 ggaatgcctg gcccttcgcc gttcagcagt tcgtccaggc tggcgagcgt ttccgcccct
    10861 tccagttgct gatattcgag ataatcttca tgtgagatcc cttccacggc cagttctgac
    10921 cactccggtt cgtgccccag ttttttgcgc gtttcgcgaa tcagatcttt catctgatga
    10981 tatttctgtc gcagctggcg cggtcgccag tcgagagcgc gcagctcatc caggatcgcg
    11041 ccgcgaatac ggtgcacggc atagcccgca aaaccttcgt ccggcagccc gtagcggcgg
    11101 atggcattaa gcagccccat taacgccgtt tgttccatat cctgacgatc aataatgcaa
    11161 ttacactgcg gggcaagttg ccgcaccact ttacgcacca acggtaaata ggcctgcaag
    11221 tagtggctct cctgctgcgg ggttaatacc ggcgcggcag caaattcttc tgactcgaat
    11281 acatcctgta gcaggtttat atccgcctgg tgattactgg aagaccactt ttttcaccag
    11341 caaatcctta tacggtgcgc tcattttgcg acgctgaaga tctttcttta acgcgtcaga
    11401 aatgagtttg cggatggcag atattttcat gccgcgcaaa tcttcaaatt tcatttccgt
    11461 caggcattca accgcaatac tttgatacag cggctcctga tgttttattt tttccgcttc
    11521 tttgtcactg gcgaccacca tcactaattc cgccgacaaa taatggtccg cgccgttgtt
    11581 gtcatgcagc gtgacaatgg tttccggcaa cgtcacaaac acgcttttgc tttcatccag
    11641 ggtttccacc gtttccgtct gcgcggcggc agtcggtttt ccttttccgg cataatgatg
    11701 ccagacaccc catccagctc cggcccccac caccaacgcc agaacggcgc tgaccacgcc
    11761 cgccaccacg attttcttca tattgccttt acactttgat caaaacggat tcgttctgtt
    11821 gaaattgagg cgcatcatca agctgtaagg cggcactcac ctcttcctgc tgatggcccg
    11881 actgttgttg ttgctgcgac tgcccttcgg aggaaacctg tacgttcacc tccataaaat
    11941 tttgcgccgt cagatgctgg cggagatcgt cgctaaactg ctgtaacgcg cggcaaactt
    12001 cactttggtt cgcgccgatg tgcaccatca gtttgccgtt gtcgagttgt acggcgatct
    12061 cgagcttgcc aagcgacggt ggatcgagac ggatggtgga gatctgctgt tgtttgttga
    12121 gctgaaagtg gatctgatct ttcaaaagcg tcgtcagttt ttcgcccagt tcgtccatcg
    12181 aaagcgtggc tggtttggcg acaatctcct gatgatcgct gagcagctcc tgcgtcttaa
    12241 cctgcaacac cgcacgcgca ataaccggtt cactgtccgg cactttttcc gttttacgct
    12301 cgacctgcgg gcgcgcggtc acacgggtta gttcagattt cgggcgagct ggctgtgttg
    12361 agacgcgcgg ctgcgtcggc gtaatggcat gtaaatcttc actagcgtaa gtcgccagtc
    12421 tggcctgctg ttcgggcttc gcctgcggca actgactgat cagttcctgc aactgcggcg
    12481 gcaacggcgt gagttcctgc tgttgcgtct ggcgttgcgg cgcgttttgc accaccgctt
    12541 tcgtcaattg ctggataacc ggcgatgttg cgacgtttcg cggctgcggc gtgtcctgat
    12601 gcggcgcggc aggttgcgga agtaacagcg ccattagcgc ctgcatcgcc accggatcgt
    12661 tctcctgcgg gcgcgtcgcc tgttgctgac tggtttttgc gctatgaacg cgctccgcca
    12721 ccgccgcgac cgccattttc ggcagggtaa acgcgggcgc gttttcaccg ctcaccggcg
    12781 gtaaaatatc cgccttcaac gaagcggttt ccgcaagcgt tccgagcgtg gcaagcaatg
    12841 tcggattcat gacttctccg aaagtaaatt aacaattgag taggccagta cgccttcctg
    12901 atgccgctga tgctgttcca tacgcgcttt caacacttct gcgcgctgct cgcgccgttt
    12961 aatcagggcg ttaaacgcgt catacagtcg cgctttcact gcatccaact gcgccgcatc
    13021 ggtttccgcc gccgcgtaat cgtgaaactg gcgtatcaac tgctgatgca gtgccggaag
    13081 gcgtcgccag cgttgcttgt cgagggcttc attcatcgcc tcgaccagtg cgaaaagttt
    13141 cctgcgttgt ttttccatcg tcttaaccca gcttgcccga aagcccttcc cagccttcgc
    13201 gcaggttgct taagatcagc atcacttcat cgattttctc tgccgacagt tcgccgctgg
    13261 cctcatacaa tcggtaaacg cagtggtcat agagtcgcga aaggttgacc accagctcgc
    13321 cgccagtttc aaactccagc gaactggtga gcgcattgag aatgtcgatg catttattga
    13381 tgctctgggc tttcttctcg aaacgccgcc cttcaatatg gcttttggcg cgttccagtt
    13441 cgtccatcag gccagaaaac agcaccagca ccagttccag aggcgatgcc gccgcagtac
    13501 gcgccgcgag atcgatctct ttatattgcg aatagccgtc ctgtacttgg tacatcttta
    13561 gggatattcc ttccgagtta cgcgaatgcc gccatgctgg ctttcatggt gtacacctcg
    13621 accagcgtgt tggtgtactc ctcgagataa cggtcgtagt tggcgttata ggtatcagta
    13681 agctgatcgc cttcgtcctg aattttgctc atctgatcgt caatgttctg ctggcgcagg
    13741 gtgatgatgc cgttgctgga gtccgtgtag gtgttaataa ggtcgtccat ctgcgccacc
    13801 atgctgttgt cgccaacgaa aatagaggtc agaccgtcag gatttttcgc catttcgtcg
    13861 ttgaactggt cggagtcaat ctgtaagtgg ccgtgagaat cgagggtgat gccatagtca
    13921 acaatcgaca cgccgttgta gctggcatgg gcgatgtcat cgagctggtt cgccagtgaa
    13981 ctgagtccgg cgtcgcctgc aaataccccg gcgctggtac tgtcatcacc gtgggtggtt
    14041 agcgaatcaa ccgtatcaat caaggtgtta taggcatcga caaaggtctg tactttctcc
    14101 tggctggcgc tggaatcttc gctgatgtta aaggtagtga gatcgctgtc agaatcgctg
    14161 acttcggtga aagtcatggt gacaccgggg atcacatcat caaacgtatt gctgctgttg
    14221 gtgatctcag gccccgtggc gctgccaaga tgaataatcg cgtcctgagc ggaggaaaca
    14281 tccgtggcaa cgggcgcgct ggtgctatcg ttgctggcat catgccccgt tacgctcacc
    14341 gaaaacgcgc tctgcgcccc ggtgctatcc gaggtgagca taatcgttgt ggtgccgtcg
    14401 gtttttacca gcgccgccga cacgcccgga ttatcatcgg agtcgttaat cgcattcacc
    14461 agttcactgg cgtcgataaa accgtcgccg tcaccattct gatctgccgc cgacagatcg
    14521 atatccatgg tgctgtcgcc catcgtcagt tcaaaagtgc cggtggcgga aaaggcgtcg
    14581 tcgcccatac tgaacgtggt ttgctgcccc tgtgccagtt gctcaacgaa aaatgagtag
    14641 cttcccgcct gcgcctggga attggcggaa acggtcgccg agtcattatt gctggtggcg
    14701 gcaaacgtca ccgggccgtc ggtatcgctg ttcagcgcgt caacggcgct ctgaaaatcg
    14761 ctcaacgctg aactgagcga atccaggccg ctgctttcag catccagctc tgtttgcttc
    14821 tcctgcaagt ttgccgcctg agtggcgaca tccgcatacg cgatttcttg cgcaatggtt
    14881 cttgggttga tcattgatga ctccggataa cgatgtcagg ttattggcaa aaggcgtgcc
    14941 aatatttttt atgtgaataa tcaatagatt aaaaaacagg cggaaatggc gtttccggtc
    15001 tggcggaaat ggcgtttccg gtctggcgga aatggcgttt ccggtctggc ggaaaaaagc
    15061 ctgccgcaga aagcacaaca ccgcccgcag gcggtgttgt tcgtagtccg aataaggcgt
    15121 ttacgccgca tccggcaagt ccgccattac tgcagcaggc tggaaaccat gctggacatg
    15181 ctgttggact gtttcagcat ggtgatgcca gtctgcatca gcacctgctg tttggtcata
    15241 ttggacgctt ccgtcgcgta gtcggtatcc ataatgttac cgatagcgac ttcggtgttg
    15301 tcctgcatac tggtcaggtt gttggcggtg tcgttcagac ggttgataga cgcacccagc
    15361 ttggactgga tctgcgaaac gtcatccatc gcggtagaga tgctggtgat catctggtta
    15421 gccgagccag acgcggtcag ctcagtaccg ccagaaacac ctgcagtacc cgtggtgtct
    15481 gcctgatccg cgctaaagga gttgctgata gcagaaagat cggtcaccag cgtgttcagt
    15541 tggctggaga tgttcacggt catggtgtcg gaagattccg cgcccacctg gaaagttacc
    15601 gcgctctgga acagaccgtc agtaccggtg ttgctggtac cggaaacacc gaacaggttg
    15661 gtgccgccgt aagtggtgtt ttgcagcatg tcagacatct gctgacccag ttcgtcgtat
    15721 tcgtcctgca tcgcctgcag atcgtcgtcg ctgtaggtgc cgttcgctgc ctgagttgac
    15781 aggtctttca tacgacccag cacatcagac atttcatcga acatactatc ggcggtttgc
    15841 agcatcgcgg ttgcgtcatt gatgttgctc agcgccacgc ccataccgct ggactgtgct
    15901 gtcagacggt tagcaatttg tttgcctgcg gcgtcatcgg cagaagagtt aatccggtta
    15961 cctgtcgcca ggcgttccat agatgtagac aaagaagagc tgcttttgct gattgcatta
    16021 acggcggcca ttgaggcgtt attggtgtta atagataaca tgatactgct ccttgggatt
    16081 aatcgttttc gtttcgatac cctgtaaaaa cgacacctaa ggagcaaact taaaaccgcc
    16141 gttaaaaaaa tttcactgcg atttatctga tgccactttt agctaaaaaa agcagagttt
    16201 aaattcagat aaaaagatta ttagcaatat ctgccacatg gccaggtatc aaaatatgtc
    16261 tttatcaaac gcttgatata gttaagatta ccatcaatta acgcgttttc ttctcaatgt
    16321 actgatgcca aaatttacat tgcgttgcaa atagtacaca tttggctatt gtaccagtcc
    16381 tgtcatttcg ttacatttcc acgccatata gtgattaaga ataatcctat taccataaaa
    16441 tattagttat cacgcagaaa aataatcttc tcagggacgt ataagactgt gctttatgca
    16501 aagcactatc aggaaacatt tatcccttgc taaggtaaac tcaactatga gtattattat
    16561 caacaactgg cggatggacc cgtcgcttaa tgccttaata cattgcgaaa cgggtgaaac
    16621 acgtcgtctc ggcgaatacc attttatttt gctggaaaca ttagcaaaaa atgccgatgt
    16681 ggttttatcg cgatcttatt tatgtgccga agtatggaag aaccgcattg tcggcggaaa
    16741 cagcctgccg acggcgatcc atgcgttgcg cgttgctatc gatgatgatg gcaaacaaca
    16801 gaacattatt aaaacaattc cgaagaaagg ttacttatgc aataaagagt atgtgtcttt
    16861 gccggactca tcgcctgcaa aaaaactgat tattaccgac cagatacagg aaacagtacc
    16921 agaagagata tcttctacaa caccgcctgt gcctgttaga aaaaaacata aagtaataat
    16981 ggggctagcg ttagccgcgg ctgttatatt tattggctcg accgtggggt attcacactt
    17041 gaaaagtaca cctgatgccc cgcaactggt aaaagagtca attaacagtc caagaataaa
    17101 aatatttcac cttagttctg gaaaagaaaa taattccgta ccgttactgt cacaaaccct
    17161 cgcaccagga aaagacaaac tcgataattt attgtcagcg cataatatga caatgacaac
    17221 ctattataaa tatgttcgta accgactgga aagtgatatt gttctgcgca accagtgcaa
    17281 tggtagctgg caattaactt ttaatgtgga aggctggcag aacagcgata ttaatagcgc
    17341 gatgtatcag aatttagaga agttgttaaa tactttgcag aaatgctgat gagtgccaat
    17401 atatgccgga tgcgacgcta aacgcgtctt atccggctga taattacccc aataaattcc
    17461 tcaccgtctg gctggaaatt cgcgcctgac cattcaccgc ctgcaataaa acctgatagt
    17521 tatgattcgc gctatccagc acgccgccga ggttttccga cgcctgcacc gcgttttccg
    17581 tttgcgggaa gcgcgacatg ccgtcgatca actgccgcgc cttttcctgc tgtaccgcca
    17641 tctgcgcacg ctgctgatta atgctttcta acgtctgaga aattcccgca ccgccctgtt
    17701 gcaggctttg agctaaacgg tctgcctcag acggctcggc aaaagttttc agcggcataa
    17761 acgccgactt atcgccatcg gtgcgcacgc ttaaggtacg ttcgatatgg ggccactgtt
    17821 tttcggtggt ggagaaactg atgccgtccg cttgctgatg tagctgcacg cccacgcggc
    17881 gcagggcgtt ggtcagccgc gtctgatact gcgcggcgct gtcatcctca gacagcatca
    17941 ccgccgaaag ctgagtctgc cgcccgtcag agacgctgaa catgcgcgtt cccggcgtgg
    18001 gattgtgtac cagattcgcc tgatccggcg agtgaaaggt cacccgcgct tcgccctgta
    18061 acaccggctg caactggcgg tccaccgcac cgccggaaag cgcggtgcgt ttgtccagca
    18121 tctgcatcag cgccgtattc tgcgcctgcc cgcctttacg ctggctgtga cgatagtcga
    18181 gcaactgctg ctccagttgc ccgagataat gatccgcctg ctgcaacgtg gtgagctggt
    18241 cgttaagctg cacgctgtaa cgctgcgggc gagtggtaat cagcggcgag gccggatatt
    18301 cactcgccgt cgccgggagt ttttgccgca ccgcctgagt gggcgcaacg gttgacgacg
    18361 ataccgcagc gctatgcgcg ccgccggtgg cgagtgatga agatgtaagt ccgacctgca
    18421 tactgttcct gaaagagtta gagaacgctg aacaaattca actcactcac tttcgagtaa
    18481 gtcttgaggg taacttccat tgccacttcc agtccggtaa aggtgatcga cgccgggccg
    18541 taatcgagat cctgtaatga accaatcagc tcatcattag aggtggagat atcggtctgc
    18601 gcatcgctca gcatcgacat ggtattttgc gtctcgccga gtgacgcaat cgaggcgttg
    18661 aggtcgtccg aggcggtgtc caccacatca acggcattct gaatatcgct ctgcacctgc
    18721 ggatctgccg ggtcgacgtc cgggttttgc aactcctggg atagggaatt cagggtgttg
    18781 agcacgtcta aattgctgcc aaagaaatca ctcgccgcca cgttggtgtc cacttccacg
    18841 ccgttcgaca ccgtggtctg gcggtggtcg ctgttaccct gatagctgta gctgtcggtg
    18901 acgccatcgc cgtcatcgtc cactgccaca atcggcggct gatcgttgat ggtgccgcca
    18961 aacacgtagt gcccgtcttc gtcctgataa ttgagcgccg ccaccattga ttcggtgagc
    19021 gactcaatat cctgcccgag gcttgcgagt gaatcggcgg tattggtgcc attcgccgct
    19081 tccagcaggt cgtcgcgcac cgccagcagg ctgttgttga cgccatcaag gatcgactcc
    19141 tgctggctta agcccgccga cgcggagtcg atattgctct gatactgctc aatcgccgac
    19201 tgctggcggt ttaactgggt gattcgcgtg gcggcgatcg ggtcatccga cggctgcaaa
    19261 atctgcttgc cggttgccat ctgctcgaca acatgcgcca gatcgccaga gagattatta
    19321 aaactttgcg tcatcgagac gtaggtttgt tgggtagtta ctcgcatttt cgcgctcccg
    19381 cttagctgca catttccagc accgaatcga aaatctcggc cccggcggaa atgactttca
    19441 gattggcttc ataaatttgt tgataggtga tcaggttcac cgcctcttcg tccatgctga
    19501 cgccgctgac gctgctctgc tggttttgcg ctgcggaata gacattggac gcggcatcga
    19561 cttccgtctg gttttgctga ctgtaaatgc cgatattgct gatgattgac gagcacgcct
    19621 gcccgaccgt cacgctgccg aggttggcta tttccagcgg ttcggtggag atattgatca
    19681 gcgcctgcag gttgtcgctg ttgccgcttt cgtccggtga actggaaaac gccagctcat
    19741 cggcggtgat attcgggtta acggtcagcg ggccatcggc attgctggcg tcataaataa
    19801 acagcggctc gccggggtta ccgttgagat cgtagccctg cgccagctgg ttgttgaccg
    19861 catcggcgaa ctgcgacgcc atgctgttga tggtgtcggt cagcggcgtc agcacatcgt
    19921 tttgataatc aaacagtgcg cctaatgaac cgccggtgtc ggtggtcatc gtcgaggtgg
    19981 tgccagcgaa ggtcagcgac atggtcgacg tgccatcggc gttggtttcc agcgcgatgg
    20041 tcgaactttg ctgcccactc accagcggct gaccgttttt cagggtaacg ttgtagttgc
    20101 cctagtcgtc gatattgacc tgcacatcca tcatcccgct cagttcttcc accatctggt
    20161 cgcgggcgtc gtacagcgcg gaggcgttat cgccattggc ttcagcctga gcgatttgct
    20221 ggttatagct ggcgatgccg ctggtgagcg tattgatttg cgacaccatc gcctgctgct
    20281 ggctgatgat ttccgtgctt tgcgagtcga tgtaatccag cgtgttatca atacgcaacg
    20341 acagcgcccc ggcttcactg atcacctgct cgcgcagggc ggaatcatcg gggctggtgg
    20401 ttgcttcgtt aagggcggcg aagaagttat cgaagccgcc gctcaggctg ctgttatcgt
    20461 cgctcagtac cgcttccagt tggctgagat acccctgctg ggtgctgtaa tagccgtaat
    20521 cgctggcggc ataccacacc tgattcacct gatactggtt ggagacgcgg cgaatgctgt
    20581 cgacctgtac gccgttaccg gcgctgttcg gcgaaccgcc gctggcaccg atggtgctga
    20641 tctccgccac ctgacgggtg tagcccgtgg tcatggcgtt ggcggtgttt tgcgccgtga
    20701 cgttaagctc cacctgcgcg gttgatgcac cgctgtagcc gatgttaatc atgtccatgt
    20761 tggatcctta ccggtaaatc gtgccggtta aaagcggcgt aaagttgggg attcttaagt
    20821 tccgtaaacc gggtgccgaa cgccggatgc ggcgtggacg ccttatccgg cctacatacg
    20881 ggcgactcgt agatactgat aagacgcgac agcgtcgcat cgtgcaccga acgccgaagc
    20941 tcatttatgc ttcggcgaca actgcttcac aatcatcgcc gcaatgccaa tcccgtgctg
    21001 ctgcgccagg gtgttacaca actcatcgtc ataaaacccc tgcatcatgc gcaccgaatc
    21061 gctgttaaac gggttatctt ttgaatcaaa caactcgttg gttttccgca tctctttcag
    21121 catgttgcgt aaaaaaatcg cctcaaactg ttcggcggcc tgcttgatat cattggcctg
    21181 aaccttcggc cccattaacg cgtcgctgcc gtcaatcccg ccgctggcgt tcactttcat
    21241 cagatcacct ccaggtcggc atccagcgcg cccgcctcat gcagcgcctg caaaatcgac
    21301 ataatgtcgt ccggcgtcgc gcctaaactg ttcagcgcat tgacgagcgt tttcaggctg
    21361 ctcgactccg gcaaactcac cacgcccgga cgggcatgat tcaccgcgat attgctctgc
    21421 ggcgtaaccg ccgtgcgccc acccgcaaag gcattcggct ggctgacgtt gctggtttcg
    21481 ttaatcgaaa cggtcaggct gccgtgcgac accgccgccg cgtgtagcgc aacgccatcg
    21541 cccattacca ccgtgccggt acgcgagtta aacaccacgc gagcgcggat tttctccgcc
    21601 tgcacctgca catcgtccag ttgcgacata aatgccaccc gcgcgccggg gctggtgggc
    21661 gcgcgcacgg taacgttggt ggaactctgc gcggtggcaa taccgccaaa cgaggcgttg
    21721 atcgccgccg caatgttgtt ggcgtcttta aacgacgggc gtttcaggtt gagggtgatg
    21781 gtatcgccca tctgaaaatc gctggggatc tcgcgctcca ccgttgcgcc gttaggaatt
    21841 aacccggcgg ttggcgtatt aaccgttacg ctggaaccgc tggccccggt ggcgttcatc
    21901 ccgcccacca ccacgctgcc ctgtgccagg gcgtaaactt ccccatcagc gccgtgcagt
    21961 tgggtcagca gcaaagtgcc gccgcgaata cttttggcat cgccaatcga cgaaacggtg
    22021 acatcaatgg tctgcccgcg cgagtacatc ggcggcagcg tggcgctcac cgccaccgcg
    22081 gcgacgtttt tcaccttcgg gtcgatttta ttcggcagtt gcacgccaaa ctggcgcagc
    22141 atattggtga tggtctggtt ggtgaatttg acctggtttt tatcaccggt gccgtcgagg
    22201 ccgaccacca ggctatagcc caccagttgg ttttcgcgca cgccctgtac gttgaccagc
    22261 gactccagcg attgcgccag cgccacacca ggcagcgcca ggctcacggc gataactgcg
    22321 gcctttatcc agttttgcat tgcactctcc ggcattaaat cgggaacaga ggatggttaa
    22381 acagccgcgt cagccagcct gcggcgttgg cgtcgcttaa cgcgccgcgt ccggcatagg
    22441 agatgcgggc atcggcgatc cgctgggagg aaaccgagtt gtcgttctgg atgtcgtcgg
    22501 cgcgcaccag gccgcttaag cggatgtact catcgccctg gttgagggtg agccatttct
    22561 cgccgcgaat ttccaggatg ccgttgggct gcaccgcatg gacggaaacg gtgatctccc
    22621 cgcgcaggct gttctgctgc tgggaagtgg cgctgccgtc aaagtcgcgg ttggcgtcga
    22681 tagagccgga aagcttatct ttagtgtgac taaaaatggt cggtgcgccg atatcgacgg
    22741 tgttgctttt gccgaagttg gttttcgcct gtttgctcga ctgggtggat tcttcgagga
    22801 tcaccgtcag gatatcgccc acgcgataag cccgccgatc ggcggtcagc gaccagttgt
    22861 agccagtttc aaatacaccg ccagcacgcc cgtctgccgg aggcggtgct tcggttttcg
    22921 gtggcgcaaa gtatgcgtcg tcttttttga ccagaatggc ctgcgattca cacccgctaa
    22981 gcaggggaag cagcgccacc agccagagat aatttttcac gttctgcctg tttaaataca
    23041 gtgagttatg cgcttttgta ggtcggataa gacgcgtcag cgtcgcatcc gacatttcgg
    23101 ccttacagcg tctgactgat gtactgcaac atatcgtccg cagccgacac cattttggcg
    23161 ttcatctcgt aagcccgttg cactgtaatc atcgccacca tttcgttgac gatatctacg
    23221 ttcgagcctt cgagggcatt gtcctgcaaa gtgccaagcc catcttcgcc cggcacacct
    23281 tcggtcggct gaccgctggc ggcggtttcc agatacaggt tgtcaccctc agcggaaagc
    23341 ccagccgggt tagcgaagtt aaccagcgtc agttgcccta actcggtcac gtcgctgtcg
    23401 ccaggaagaa tcgccgtcac cgtgccgtcc tcaccaaacg caacattcgt cgcccccgcc
    23461 ggaacgtcga tttccggctg caatggcaag ccctgtgagt tggtcagtac gccatcggcg
    23521 ttcacctgca aattgccgtc gcgggtatag gcgatatcgc cgttggcctt ttgcacctgc
    23581 aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc gacgttgcct
    23641 tcggtgaagg ttttctgggt gccgacaatg cgcacgccgc taccgaattg cagcccggtc
    23701 ggcatgatgt tgttctggtc gagcatcgcg cccggcgctt ccagggtctg gtagaacaga
    23761 tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc gatgttgttg
    23821 gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat ccataatgct
    23881 gcgttcatgg tggtaaattc cgtgttattt ttgggccgcg gttgccggat gcggcgtgaa
    23941 cgccttatcc ggcctacgat gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg
    24001 ctgatatctt cggcggtttt catcatcttg atctgtgctt cgaactggcg gttcatggcg
    24061 atggacgaca tcatttcgct taccgccgag acgttgctgc cctcgagaaa accgccgctg
    24121 actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag catcccttca
    24181 gcatttttct ccaggttagc caccgggata tccaccagct tcaggcgatc gatatccatc
    24241 gtcgcggtca catcgccgtc atcgggcgtc accgacagcg tgccgtcgct gccaaacgaa
    24301 gcaatggcgt tcggcggcag aatgatcggc ccgttatccc ccagcaccag attgccgtcg
    24361 atggtcagtt gcccctggtc gtcctgctga atgttgccgt tgcgggtata gacttcgtta
    24421 ccgtttttat cctgcaccgc aatgtaaccc gcgccctgaa tcgccacgtc cagcgggcgt
    24481 tcggttttct ccgcaacacc cgtgctgtcg ttcacgccgc tggccccttc ctgcgccata
    24541 tagcgcgtgt cgaagccgcc gcctttcacc ttgtcgttgg ttgccattgc catatcggcg
    24601 cgaaaaccat tggtgttgac gttcgccagg ttgttggcgc taatttgctg ctcatacagg
    24661 gtctgagaag caccgctcag ggcggtataa atcaggcggt ccattacatt gcctggaaca
    24721 gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga tagttacgct
    24781 gggcggtcat caggttgacc agttcgctgg tgatatcgac gttagagctt tcgagcacgc
    24841 ccgacgacag cgtaccgcag gtgccggagc cgggaacgcc aatcagcggg gtgccggatt
    24901 cgcccgtttg tacccatgcg gtgccgctca ctgcctccag gccgttttca ttcgggaaag
    24961 tcgccagcac cacctgcccc tgcaacatgc gctcgccgtt gctgtaggtg gcgtaaactt
    25021 tgccatcgtc atcaacctga acaccgtttt gcgtggcgga ggcgtagccg ttggcggcgt
    25081 tggtggtgac cgaaaattca gagccgtatt gcgtacaggt ggagtaatcg acggttaagt
    25141 cgacgggcgc ggcggcgtcg gtttgaaatt caatggtctg cggcgtggtt ggagaggtca
    25201 gcttcccggt gttcggatcg aaggttaacg tcgtcgccgg aacgccggtc tgctgctgac
    25261 cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttggtgaag tactggcata
    25321 ccgaatgttc gttgcctaat gagtcataca ccgtggtggt gtagctgtcg gtgtacgaac
    25381 tgctgttagt cgcgtcgaac ggaacggtgg tgcgatcgat agcttcatcg ctggcgttga
    25441 agttagcggt aaaggtcagg ctgctgctgg cctgcgccgg gatattgccg gttttgatct
    25501 ggatatcggt gaccgtaccg gtttgcagag tgccagtgtc atcgaccgga tagccctgca
    25561 aataagcgcc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac gatccggcgc
    25621 gggtatagga gatattgccc gcgctgtcac acatgacaaa aaatccgtcg tcgttaatcg
    25681 ctaaatcgag cgcattgccg gtggagacca gcgaaccgcc acgcgaaata ctttgcgcgg
    25741 tgcccgccac gctgacgccc atcgcctggc ttccggcata catggcggaa aactgggtgg
    25801 tcatcgactt atagcccacc gttccggcgt tagcgatgtt gttactgatc ccgtccagct
    25861 gttcgttaac ggcattcagc cccgttgcgg caatttcata actcattgtg tcttcctgtt
    25921 aattgattct tacgccacat cactgtcggc atcgggtacg ccaaactggc taatcatggt
    25981 gaacggcact tcgcccacgc ctgcaacgtt aagcaccggg gaactgccgt cgagcgggat
    26041 gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc cggtattggt
    26101 caccaccgag acgtcgtaat cgcccggcgg gatgccgtaa tccgcgggat tcaccgagaa
    26161 atcaacctgg cctggtccga cggacgaact gccttctggg atcagcgaga cggtgtagtc
    26221 atcgcctgcg gcatcggtaa agtggagatc ggcggtggta caggcgtcgt ccagcgtagc
    26281 gcgaccgtta atggtctggt cgctcacctg caaggcggtg gtctgcacca taatgtcgtc
    26341 gcccaccagg ttaccgagcg ccatcacctg aatgttgctc atcagcaccg cgttggtgtt
    26401 ggcctgcacg ctcatctctt ccatcatcgc cacctgcgcc atcgacgaca gctggtcgat
    26461 gtattcggta gcgtcggtcg ggtcggtcgg gtcctggttt tggatctcgg cgacaaacag
    26521 cgtcaggaag gtatcgacag gcgagctatc gctgctgctt tgcgtgtcgc tggccgccac
    26581 attattgctt tccgccgtgg cggtcggctg cggcgattgc gcattcagcg ccagggtatt
    26641 catcaggctt ctcccagttt cagcaaactt tgctgcatac ttttcacgtt gttgagcaca
    26701 tcgacgttgg tttcgaaatc gcgcgaggcc gacatcatgt ccgccatctg ctccaccacg
    26761 ttgacgtcgg gataccagac gtcaccgttg gcgtcggcga gcggagaatg cggttcatag
    26821 cgtttcaccg ccccgccggt ttgcagcacg tcctgtacct gcacgctggc accgtcaatg
    26881 gcatgacgat gtgtacccgc cagcaggctg tggtgataca ccgcggcaaa caccgggctg
    26941 cgcgctttat acgcctgcgc ctcgctgctg gcgggcgcat tggcattcgc caggttactg
    27001 gcaacggtat tcagacgtag cgtttgcgcc gtcatcgccg agccggaaat ctgatagata
    27061 tcagtaaatg acatgcttat ttcccttcaa tggcctctct gatgccgctg atttgcagat
    27121 tcagaaaggt caaactgctt tgataatcca tactattttg tgaaaaccgc gcctgctcgc
    27181 tgttcagttc cacggtgttg ccatcttccg acggctgcat cggcacgcgg tactggacat
    27241 ccaccgccgc gttatttgcc cgttgcatct ccctggcaaa atcaatatct ttagccttga
    27301 aatttggcgt atcgacgttc gccagattcg cagtcagtag ctcggtcctc tcaagacgta
    27361 acttcactgc ctgcggatgt actcccaatg cctgctgaaa actgatcccc atttcctgca
    27421 tcactcctgc ttcaatccgc atacgctaag caatcgccat gccatttttt aagtcactga
    27481 tgagaaaggg tttatgagtt ttcaattttg caggcggaag ttttacttcc tcctggcgct
    27541 ggccttaccg ggctacgccg ccgtccatcc cgtgcaacac agcgcgcggg aacaagtcaa
    27601 cgcccaggtg ctgaatgccg cgagccaaaa aattgaatcg ctggcgcagc agcggcagtg
    27661 gcatgactat cgctacacct ttaagatcta cattccctcg cagattgcga ccgccgcgcc
    27721 ctgtactaaa acacccggcg tcacgctgac ttcgcctgcg gaaatcgccc tcaaccggat
    27781 gaacttcacc gttagttgcc cgcaaagctg gcagatgaac gtggcggtgc gcccggatgt
    27841 actggtgccg gtggtaatga cgaagtcact ggttgcccgc gatatgccgc tcaccgcgaa
    27901 tgatgtggag cttaagccct acaacgtcag cgcccagcgc cgcgatgtgt tgatggagct
    27961 tgatgacgcg attggtttaa gcagtaagca cgcattgcaa ccaggcagac ccatcaccaa
    28021 agaagaactg gtgtctccgg tgctggtcgg gcgcgatcag ccggtgatga ttgtttacca
    28081 gagcgcgggg atcaccgcct cgatgcccgg cgtggcgctg aaaaatggtc gtaagggcga
    28141 gatggtgaaa gtgcgcaatg ccagcagcca gcgggtcatc agcgcgatgg tggcggaaag
    28201 cggcgtggtg acaacggtaa gcgcggagta atttgcgcag gcgattaaag ttttgccgcc
    28261 tgttaaccgc tttaccgcag taacccaccc aaataccgag acgaggttgc gatgaaaatc
    28321 accccgacta tgcctggcaa tcgcccgaca gccacaacgg caggctccag ccagccgcgc
    28381 aaaaccacgg tgaccaccac cacgacgctg agcgccgacg acattactca ggcgggattg
    28441 cagtctgccc agcaaacgct gaacgaccag caagagagcg atatcgacag caacaaagtg
    28501 gcgcaagtgc aggcgatgct ggcgtccggc agcccgcaag tggacactga acagttagcc
    28561 agcgacatgc tgagtttttt ccagaactaa gaggccgatg aagtgagtat ccggttacaa
    28621 caggtgaagg cgttgttgca gggcattcgt gacgatgacg cgctgtatga ttccctgcgc
    28681 gaacttctcc agcgtcagcg gatctgcatg attcgccgcg ccagcgaaga gttgctggcg
    28741 gtaaacgatg agataaccca tcactatgag cagttgcatg gacatagcca tcagcggcat
    28801 agcctgctga aaatgctcgg tgttagcgta aatcgtgacg gtctggcgca ggttttcacc
    28861 tggcttccgg ctgttcaaaa agcggctgca caacagttgt ggcaacgcct tgagcaaaaa
    28921 gcggaacgct gcaaagccta caacgacaaa aacggcgagc tgttgattcg ccagtatgaa
    28981 tttattcagt cgtttttagg cagcgaagca gattttctct atcaggaata acgcgaatac
    29041 cttccccata gcggaaatgg attttccgcc tgatttccaa tcccttgttt ttaattacaa
    29101 aaatccccgg cacgcttatt gcatatcctg cacagcatta aagactggaa gccacctatg
    29161 agttttattg aatgttacga accggaatac gtgcgcaaat ttttagcgcg tcaccccggt
    29221 tcgcctttat acgtccgcaa ggtgtggaaa aacgagattc gcctgagcct gtcattgacc
    29281 gatattgcca gctgcgaagc ggtgctcaac gatgtcaggg cctttgattt acagctcacc
    29341 taccgccctg tggaagataa ttccgcagag ctttccgcgc gcgatgcgat agtaaaccag
    29401 gtgattttga gtaccctcac gctccgtgat ttaaccccgg agctttccct gtatgctgtc
    29461 gggattttgc tctcctgcgc cagcaaaatg ccgggcagag acggagacat cctggcccgt
    29521 ttgacgacgc tgccacaggc gctggccgat catgcgaaaa aaggtacgct acaggcacag
    29581 tacgcccagc tcccgcccgt gccacagctg gcgcgccagt tgatgacgct gctcggcggt
    29641 tgtgcgtttg actggtcgat tttgccggaa agcccgcgca aagcctcgct gccgttgcag
    29701 gttactttac tgacgctgca tgacgccaac agcgaagccc tgttacaaca gcagcttcag
    29761 acgcagtggc aaaccacctg gcaacagcat tttgccaccg cgccctggat gatgcgtaac
    29821 tggctgattt accgcgttta ccacgatatg attgggcaga ctgacggggc ggattatttc
    29881 ccgctggtgt gcgattttta tctcctccgc acgttaatca gtttatggac gctggatggt
    29941 tctccgctgc ggcaggagga tattttcgcc ctctttgcca tgtttgaacg ctggcgagcc
    30001 agtgaaaatg cgctgttggt tcgccagcaa attcagtcgt tatgtgccgc cgacccgctg
    30061 ctttcagcgt tttcgctgct aacgtgagtc gctctgcgcg cgctttgccc ctcaccctaa
    30121 ccctctcccc aaaggggcga ggggaccgat cgagcacggt gttaaaatcg gcgctgattt
    30181 tctccctctc cctgtgggag agggtcgggg tgagggcatc agcgcgcacg ttcatcacat
    30241 tcacacatta actcacccaa acaccatgcc aacaccaacc atcatcaaaa cactcgccac
    30301 ccaccgcgaa aaaccgtttg tcagcgtcgt cacgccaacc tggaaccgcg gcgcattcct
    30361 gccgtacctg ctctatatgt accgctatca ggattacccg gcagaccgcc gcgaactcat
    30421 catcctcgac gattccccac aaagccatca acacatcatc gaccgcctga ccaatggcac
    30481 gccggaagcc tttaacatcc gctacatcca tcacccggaa aaactgccgc tgggcaaaaa
    30541 acgcaacatg ctcaacgaac tggcgcgcgg cgaatacatc ctctgcatgg atgatgacga
    30601 ctactatccg gcagataaaa tctcttacac catcgctatg atgcaaaaac accgggcgct
    30661 catctccggt tccgaccaga tccccatctg gtacagccat atcaaccgta tctttcaaag
    30721 ccgcagcttt ggcccacata atatcctcaa cggcaccttt tgctatcacc gtaactacct
    30781 gaaaaagcac cgttacgacg atgattgcaa tttaggcgaa gagaaaagtt tcaccgacaa
    30841 tttcagcgtt aacccgctgc aactgcccgg cgagcgcacg attttgtgta tctcgcatag
    30901 ccacaacacc tttgataaag actttattat cggggccagc acgccggtaa acacggcgct
    30961 tacggatatc gtccgcgatc cgctgctgcg caatgcttat ctgagtctgc ataacgccac
    31021 tcatcaccag gcgatcaacc atcaggcgat cgatcaggtg gtcgtcgtga atctggataa
    31081 acgcccggat cgcttacaac agatccgtga agaactggca ttgctgcata ttccacccga
    31141 gaagatcacc cgccttgccg ccagtgaaga tgaaaacggt caacggggcc gccgtcagtc
    31201 gcacttgcag gcgctgcgtc tggcgcaaca gcacggctgg caaaactatc tgttactgga
    31261 agatgacgcg gttattctca agcaggagaa acatattcag gtactgaaca cgctgctggc
    31321 gagcctggcg aaaattccct ggcaggtgat gatcctcggc ggcgaaattt cgcaaggaac
    31381 gatgctaaaa agtttgcctg ggctggtgca cgcgcgggac tgccgcaaag tgtgcgctta
    31441 tctggtgaac agccgctact acccgcaact ggcgcagcag atgagcaacg atgagcactc
    31501 gctggaagac tgctggcaac cgctgctacg cgccgacaaa tggctggcct gttacccgag
    31561 cctttgttat cagcgaccgg gctttagcga tatccagaaa aaaacgactg ataatattgc
    31621 tcgctatttc aataagttac ccgtagccac caagccatcc gcgttaccca tcgccgacac
    31681 catcggcttt tttatggaaa cctcattcca ctacgcgctg taccgcccaa tcatcaccgc
    31741 cctacaggcg caagggctga gctgcacgct ggtgatcaac gaccgcgtgt ttaaaccgtt
    31801 tctggatgag atgctggaaa cactgaaaaa catcgacgat ccgcagctta aaggtatgcg
    31861 tctgtcagaa atgcagacac atggtcagcg ggtgaaatgc ctggtcagcc cctaccacac
    31921 gcctgcgctc aatggcctgg cggcggtgaa catccgcgct atgtacggct tagccaaaga
    31981 gacgtggaac cacgccgact ggaatcgttt ttaccaaagc attttgtgtt atagccacta
    32041 cagtcagcag gcgcttgcgc atttcggcag cgcaaaagtg gtcggcaacc cgcgcttcga
    32101 cgcctggcat aacggcacgt tcgatcgcgc gttgccggaa aacatccaat cagattaccg
    32161 taaacctacg gtgctctatg ccccaacgtt tggcgcatta agctccctgc cccactgggc
    32221 tgaaaaattg ggccgcttga gcggtgatgt gaacctgatt tgcaaactgc accatggcac
    32281 ctgctcacgc ccggaagaag cagcatcgct ggccctggtg cgcaggcatt taaaacagcg
    32341 caccgactcc gcccgccaca cgctggcgct gctggcaaaa gccgattacg tgctgaccga
    32401 taacagcggg tttattttcg atgctatcca cgttgataag cgggtgatcc tgctcgactt
    32461 tccgggcatg aatgatttgc tcgacggtga gaaaagttat tccacggcag agagcgccga
    32521 ccagcgaatc cgcgaaatat tgccggttgc tcatgatgtt gcagaactgc gttatttgtt
    32581 gtcagaggcg tttgactggg ggtcggtgca ggcgcggctt acggagattc gccatcacta
    32641 ttgcgatgcg tttatggatg gcaaggcggg agaacgggcg gcgattgtga ttgtggaggc
    32701 gctggggtaa accgcgcaac tgcctgatgc gcttcgctta tcaggcctac ggatcgatca
    32761 cgtcctgtag gccggataag gcgtttacgc cgcatccggc aagaaaatac atctctgttt
    32821 tagtacttta taaaagccct ctaatcacct caataatccc cgtcgtcgac accgacggcg
    32881 tgcgggggaa atagaccacc tcacaaatat acgcaaagct gtcaaacttc cctgcccagt
    32941 catcccccat cactaaaata tccgcactgt acccgcgcaa atactccgct ttctgctcta
    33001 gcgattcttc cagaaatacg ccatccacgc acgccagccc ggcgatgatc gccatgcggt
    33061 catcctgatg gtaaaccggt gccctgccct ttttggcgat gttcagggcg tcggacgata
    33121 cgccgaccag cagccgctcg cccaatgcgc gggcgcgttg taaaaggcgc agatgaccga
    33181 catgaaacac atcgaaggtg ccgaaggtga tgaccgtttt catggctact ccgctaattt
    33241 gtgccgcagc cagcactgca cggcgatgtc gtccaccgct ttttgcgctt tcgccgtggc
    33301 gctttcttgt tgctcgcggc gtttgtcgtc cagcgtcagg gcgactactt tctcttcgca
    33361 cgccgccgcc gtgaggtttt tctgcattcg cgtggctttg atattggcca gcgttttttc
    33421 ctgctcctgc caggcgatta ccttgcgcag ggtgcctttg tagccgctga cgtttttcag
    33481 cgattccacc gacaccgccg cacccgcaga tgttttctcg acaaggtagc ctaacgcctt
    33541 gatattgttg tcataacccg cgcaaatctg tttttgcttt gccagctcga cggttttgtc
    33601 tttgaccgat ttatcccgca ggcgctgcaa ctgcgtcagg gtgtcgatga tttgtcgcat
    33661 agttactgtt ctgccttttt gacaccagga aagagaattt gcaggcggct ttgcaccagt
    33721 tcgagggagg cgggttcgcg catctcctgg cgcaaaaatc gctcgatggc cgggaacatt
    33781 tttaccgctt tgtcgacgct ggcgtcggca cctgccacat aaccgccgag cgggatcaga
    33841 ggtttgatct ccatataggc ggcatagttt tgctttaacg cgcgggcgga ttgctgatgt
    33901 tccaggctgg tgacctggtt catgcagcgg ctgatcgact ggccgatatc aatggccgga
    33961 tagtgccccg cttccgccag tttgcgggtt aatacgatat gcccgtccag caccgcgcgc
    34021 gcgcagtcga caatcggatc ttgctggtcg tcaccttccg ccagcacggt gtagatggcg
    34081 gtcatcgatc ccgcgctttc gctgttgcct gcgctttcga ccagtttggg gatcatgccg
    34141 aatgccgatg gcggataacc tttggtggcg ggcggttcgc cgagcgagag ggcgatttcc
    34201 cgctgcgcca tcgcgtagcg ggtcaggaaa tccaccagca gtaagacgtg atgcccgcgg
    34261 tcgcgaaacc aggcggcgat ggagtggcac agttcggtgg ctttcagacg catcagcggc
    34321 gattcatcgg caggcgcgac gacgacgata gactttgcca gcccgtccgc gcccagcgag
    34381 tggtcgataa actctttcac ttcgcgcccg cgttccccaa tcagcccaac caccacgata
    34441 tcggctttgg tctggcgggt gatcatcccc agcagcacgc ttttcccgac gccgctgccc
    34501 gccattagtc cgacgcgctg gcctttgccg atggtcagca ggccgttgat cgctttcacg
    34561 cccacgtcca gcggttcgtc taccgaacga cgagttaagg gattaaccga gggcggcaac
    34621 ggcggcagaa ggtcgttgcc gttcaggcgg cctttgccat ccagcggttc gccgaggcca
    34681 ttcaccacgc ggcctaacca gctttcgcct atcaggatgt cgtgggcttt ttcttccggg
    34741 aagacgcgtg cgcctgccat caggccgacc ggctgtttaa acggcattaa ataggtgatg
    34801 tcgcgattaa agcccacggc ctgcgcgtcg atcagcgtgt gattggcact ttcgatgcgg
    34861 cacagctgcc cggtcattag cgggcagccg acactctcca gcaggatgcc gttaaggcgc
    34921 accagccgcc cggcgacgcg ggccagcggg atcgactcca gcgagcgcag ggcgttatcg
    34981 aacgcagaaa aatcactcac cctgcgactc cggcgtgagt gtttctttca gcgcggtcat
    35041 gcattgttca aggcgatgct cgcagccgat atccagttcg gatttgtcgg tgatcacccg
    35101 gcattcgccc ggtggcaaat ccggcgaagg cgtcagcccc cattcgctga ccttttccgg
    35161 caccgcgtcg cgcaggcggt taaactcttc cgtgctcagc attacttgca gggtttccgg
    35221 catcgccggg aaggccgcca gcgcctcttc caccagcgcc agcaactgcg tgggttgcag
    35281 cgccagttca caacggatca cctggcgggt gactttttcc accagttgca gcaaatcttc
    35341 ccgctgttta cgttcgatat gagcgaggaa gtcgttcact ttaccggtga tggcttccag
    35401 cggctgcgcc gcctcaacaa actgtttgcg cccttcctgc tgcccggcga ggctgccttc
    35461 ggtgtaaccc tggcgtcgcc cctcggcaaa accttgctga tgaccttcgc taaagccctg
    35521 ctcctggcct tcggtcattc cttgagcaaa ccctttttgc agcccttcct gaaatccgtc
    35581 catcaactgg cgctggtaat cggcggcgct aatactggta gtgagcggtt ctccggtcac
    35641 cgcacgctgg cgcggcggga aacggtgcag gcgatagcgc ccgcgcagtg tttcaatggt
    35701 catggttact ccgccgtctg ttccgcaaag agctgtagct gaatttcgcc cgcctcttcc
    35761 agttcacggg cgatgcccat gatttcgcgg cggatctgtt cgatacggct gaccggcacc
    35821 ggccccagac gcgcggtgat ggcttccagt tgctgcgcct gccgttttgg cattaccgcg
    35881 taaatcgaac ggcgcagcag ggcttcggtg cctttcagcg ccaccgccca gtcttccatc
    35941 ggcacttcgt caagcagacg tcgacgcacc tcttcgttct ggcggctgag gataaagaag
    36001 tcgtacatct catcctgaag ctgctccagc acctcttcgt cgcgctcgcg catctggtcg
    36061 aggatcacct gctggttgcc ctggaagcag ttgacgatat ccgccgcctg tttgatgcct
    36121 ttcacttttg agccgtgttc cgaaagcacc gacaggccgc gttcgatcag cctgtccagt
    36181 tcatccacca cgtcgcggtt gacgtcgttg agtttcgcca cccggtagag aatttcgttt
    36241 tgcaccgatt cgttgagata cgagagcacc gctgccgaga tttccggcgt cagaaacgcg
    36301 aggaacaccg cctgtaattg cagatgttct tcggagatca gcatcgccag ctggcgtggt
    36361 tccacccatt gcaggcgcgc catccgcgag cggatctcat cgccgtagat gccgttgatc
    36421 acactgctgg cgatctcggt gccgagggct ttgttgagaa taccctgcaa catggatcgc
    36481 gatgcgccgt tgatcccgct ctgttcgcgg aactcatcaa agaagttatt gatcactttg
    36541 cgcgccatgc tggttttcac gccggaaagc cgtgccatgt tttcgctcag gcgcaccact
    36601 tcttcgcggc tgagtttttg catcaccgtc gccgccgcct cttcgcccag acacagtagc
    36661 aggatcgccg cctgttccag gtagctgttg ctcttatttg ttgtcaattc gctcattgct
    36721 ggtgatccat tgtctgagaa cttcggctac gcgatcggtg tcgctcatgg ccagtttttg
    36781 caggaactcg agttttactt ccaggccgga actctgagac ggcaggctgt catcgccggg
    36841 cagcgacggc agatcgacgt ttttgcgctc ctcttccggc atggcaatcc acggttcgct
    36901 gacggcttcc gattctggtg tgataatcgg tgcacgtacc gccgtcagcc gtttcattac
    36961 cgggcgcacc acgaagaaca gcaacaacag cgccagcagg ccgcagccaa tcaggcgcac
    37021 ccaggccagc acgctgtcat ctttccacag cggaataatt ggttcgaccg gcaccgcctg
    37081 cggcacaaag ttaagcagtg aaagcgtcag gttatcccca cgttgcgcgt caataccggc
    37141 ggcattgttc agcaacgcgg taagctgcgc ggtctgctcc ggcttccagt ttttcagcgc
    37201 cggagcgttt tgattgagca ccaccgccac attgaggcgt ttcaccgcaa agccgggatg
    37261 ctgaatatgc tggacgctac ggtcgtaaga gtaatcgcgc ttgctttcgc tgtgtttgga
    37321 taacgcttca ggcgagcgat tttcttccgt accgttagtc atttgattcg ccgcaaccgg
    37381 cggacggttg ctgagagagc cgggcacacc catcgccacc tgattggtgt cgctgtccag
    37441 cacgctctct tcgcggttta ttttcggcgc gtcgccgtag tgttcctgag tttcgtcgat
    37501 ggtgctgagg tcgagatccg gcatcacgct gacacggtaa ttcccgctgc cgaccagcga
    37561 atccagcacg ttcgccacgc tggcgtgggt tttgtcctga atgtctttga ggatctgatc
    37621 gcgtttacgg gtagcggcag agaccgcctc gcccgcgccg atgccgtcgg tcagcagatt
    37681 tcccgactga tcaaccacgc taactttgct ggcgtgcagc cccggaatgc tgccggaaac
    37741 cagatgcaca atggcgttca cctggtccat attcagcttc gcgccgtagt gcagacgcac
    37801 caccaccgag gcgctgtttt gcggctcatc gctgaccaca aacgagcttt cttcgttgag
    37861 cgccagatgc acccgcgcgc tctccaccgc gtccagcgac ataatgctct gcgccagctc
    37921 cccttcgagg ctgcgtttat agcgcacgtt ctgcacgaac tggcggctgc caagcacttc
    37981 gtctttgtcc atcagctcgt agccgctggg caaaatcgcc tgcacgcctt tggcggcaag
    38041 ggtcatgcgc gttttcgaca gcgcctcttc cggcaccagt atctgcccgc tttgcgggtc
    38101 aatgcggtag gtgagctttt cgccgtccag cacggtgaca atctgcgaca cgggcagatt
    38161 ttcctggcgt ccgtagagcg agacgtagcc gcgattgccg gtccacagca cgctaacgat
    38221 aatcgccgtc gccgccacgg caagccccac acccgccatc agcgcccagc gtttgttatc
    38281 tgcgagacgc agtcgaaacg ccggaaacgc ctgggttaac ttctttattt gtgcattcat
    38341 agagttagat cgacatactc atcagatcgt taaatccggt ggcgattttg ttgcgcacct
    38401 gcaccagcgc ggagaaagag agcgacgcct gctggctggc gatcatcgca cctgccagat
    38461 catcgctttt gcccatgtcg atagccgttt gctgctgctc cgccacctgc tggaactggt
    38521 caacgtgacc gagcgcgccc tgcataatgc ggtgaaacga aacgtccggg tcggtggaac
    38581 tgaaggtcat ggcgggcagc accggcgcct gggcgttggc ctgcatccgc tgcacgtcct
    38641 gcatgatctg cgcctgcatg gtgctgttga ttgtggtaat gctcattttt aacttccggg
    38701 tcaggagaaa acttcgatac cgtgcttgcg catggaggcc agtcgatagc gcagtgcgcg
    38761 cggggtaata cctaacaggt cggcgatttt gctgcggttg ccctgatatt tacgcatcag
    38821 gtcagcgata tattgatact gggcgcttcg tccgtgctgg cctaaatcgc tggtttcggc
    38881 aatgtgtacg gcgggctgag caggccattg cagttcgctg tggtcggcaa tgtcattttc
    38941 cggcaggcct aaggcgtccg ggtaaatcac gccgtcgcga tttaaaatca ttccccgctg
    39001 aatggcgttc tccagctggc gcacattgcc gggccagcgg taattcagca tcgcacggcg
    39061 ggtagattct gaaagggtga tatttttcac cagcaccgtg gagtattttt ttataaatga
    39121 ctctgccagc ggaataatat cgttcagacg ttcacgcagc ggcggcatgg taataggaat
    39181 aaccgccaga cgataataga gatcttcacg aaaacgcccg gcggcgactt cctgctcaag
    39241 gtttttattg gtgcaggcaa tcaggcgaaa attgagttta atttgtcggt tgctgcctaa
    39301 tcgctcaacc tgttgttcct gcaatacgcg taatattttg gcctgtaacg ccagcggcat
    39361 atcgccaatt tcatcgagta ataaggtgcc gttattcgcc agttccattt ttcctggcac
    39421 actggcaatt gcgccggtaa atgcgccttt gtcgtagcca aataaggttg cttccagcat
    39481 gttttccggg atcgcagcgc agttcacgcc gatatagggc gcgttttcat tttcaccaaa
    39541 ggcgactgtg tgaatatatt tcgccacgca ctctttgccc gcgccggttt cgccctgaat
    39601 gagcaccggc acgttaaatg cggcgacacg ctttgccaga gtaaaagcgt taatgctgga
    39661 agcggcagtt gcaataagtt cagacatagg gtgaacgata taagtagcga caataatcag
    39721 gatgttctga agcgtatcac cggcgggatt aacaagttcg aattaaacga taataaaaat
    39781 attatttgtt tataaacaat gatttatcca tgtaggccgg ataaggcgtt tacgccgcat
    39841 ccggcaattg tgcaccggtg cctgatgcga tgcggcgcgt cttatcaggc ctacggttcg
    39901 tgcaatgtgt tatgacgatg cctgatatct cgccctttaa taatttaatt attttttcac
    39961 cctattaata atttgcccct ttcacgcccc ctctatttaa tacctcacct tgtgtcactg
    40021 ccggatttgc gtgcacaaaa tcgcccatac cattacagca gagaaagtaa gccatgctga
    40081 agtacagtaa aacgccaggc atctttaaat tagaaggtaa tcggctcgga cgcccctacc
    40141 atcgcctgcc gaccctgttt acagggaatt ttgacgtgat tgattcgcat ctgggcagct
    40201 actttctcaa aaagcatcgc agcaatatca ccctaaaaaa gattggctgc gaaatggata
    40261 ttattaataa aaacgctgaa ctgatggttt cccaggtcgg gcatctggcg tttgatattg
    40321 atcgctcttt gctgttaatg ctgctgggca atttttatgg tctggagtcc tctcttgaag
    40381 aggcgaaagc gcataacggg ctgccgacca aaaccgaaac gcggttaaaa aaccgtctgg
    40441 cgctggatat ctgcacgctg atttttaatc tgcaaacctc cggtattgcg ctgaaattaa
    40501 agctggacag cagcacggta attacccact gggcgtatca attaaccttt acccttgcgg
    40561 gcgatgaaga gagctgtttt cgtattttgc tcgacgacgc ccacaccgat tttattttaa
    40621 atctgatccg ccacagcgag cacagtaaac cgcagcaggt ggcgaaatcg gtcaataaac
    40681 cggccctgat taaagagatc atccgctccc tgccgctgac cctgaacgtc aaaatcgccg
    40741 agctatcgat gaatgtcgcg gatctcacgc agataaaagc cggggacatt ttacccatct
    40801 cgctgggaga aacgttcccg gtggcgattg ggcaatctga attatttagc gccctgattg
    40861 tggaagacaa agacaaactg tttttgtcgg agctggcggg caaaaatgag aatagccatg
    40921 agtaagcaag aagatatttt agccggggat tttggcctca ccgatgaagt tgcgcctgtc
    40981 gcaaaatccg ccgacgctga aacgctggtc acccgcctgg aagaccgttt ttccgactcg
    41041 atgacgctgc taaaacgcat tccggtgacc ttaacgctgg aagtgtcatc agtagaaata
    41101 atgttagccg acctccttaa catcgacgac gacacggtga ttgagctgga taaactcgcc
    41161 ggggagccgc tggatatcaa agtgaacaat atcctgctgg gcaaagcgga agtggtggtg
    41221 gtcaacgaga agtacggcct gcgcgtgctg gaatttaaca cccgcgatat caacgacctg
    41281 gcaccatgaa acgccgcact caactgacgc tgggccttgg gctgctggcg cttgcgccgg
    41341 tggcgttggc ccagggcggc gatattgcgc tgcttaacgt ggtcacccac ggcaacactc
    41401 aggagtacag cgtcaaaatc caggtgctga ttctgatgac cctcgtcggt ttgctgccta
    41461 cgatggtgct gatgatgacc tgctttacgc gctttattat cgtgctgtcg ctgctgcgcc
    41521 aggcgctagg gctacaacaa acgccgccaa accgcattct gattggcatt gcgctctccc
    41581 tcaccatgct ggtgatgcgc cccatctggc tcaatattta cgaccacgca gtagtgccgt
    41641 ttgaaaatga tcagattacc ctgaccgatg cgttaagcac cgccgcgacg ccactcaaac
    41701 gctttatgct ggcgcagact gacaaaaaag cgatggcgca aatcatgact atcggcggcg
    41761 cgaagggcca tgccgccgac caggatctct cgattgtggt gcccgcctat gtgttgagcg
    41821 agctgaaaac cgccttccag attggcttta tgatttacat cccgtttctg gttatcgacc
    41881 tgattgtcgc cagcgtactg atggcgatgg ggatgatgat gctgtcgcct ctgatcgttt
    41941 cgctaccctt taagctgatg ctgtttgtgc tgatcgacgg ctggtcgctg accatcggta
    42001 cactcaccac cagcattcgc gggctggggc tgggctgatg atgctgacca tagacgtagc
    42061 cgccgacatt gtcgccagcg gcataaaagt ggtgattttg ctggtttcgg tgctggtagt
    42121 gccgagcctg ctggtcggcc tgctggtgag cgtgttccag gcggtaacgc agattaacga
    42181 acagacccta agctttttgc cgaggctgat tgtcacgctg gtggtgctgg gcgtgtgcgg
    42241 taaatggatg attatccagt tgcatgatct ctgcattcac ctgtttaccc aggccgcgtt
    42301 gctggttcat tgagatgcgc acaagcgacg ttacgcaact gatggatctg gcgctggggc
    42361 tgtggtttcc ctttgtgcgc attatggcgt ttctgcgcta tgtgcctgtg ctggataaca
    42421 gcgccctgac cgtgcgcgtg cggattattt tgtcgctggc gctggcaatc atcatcacgc
    42481 cgttgatccc ccatcctgtt ccccacgacc tgatatcgct caacagcctg atcctgacga
    42541 tggagcaaat tttatggggc atgttattcg ggctgatgtt tcagtttttg tttctggcgc
    42601 tacagctggc cgggcaaatt ctctcgttca atatggggat gagcatggca gtgatgaacg
    42661 acccgagcag tggcgcatcg accaccgtgc tggcggagct catcaacgtc tacgcgatcc
    42721 tgctgttttt tgccatggac gggcatctgc tgctggtgag tgtgctgtat aagggcttta
    42781 cctactggcc gattggcaac gcgctgcatc cgcaaaccct gcgcaccatc gcgctggcct
    42841 ttagctgggt gctggcgtcc gcatcactgc tggcgctgcc aaccactttt atcatgctga
    42901 ttgtgcaggg ctgctttggc ctgctcaacc gcattgcgcc gccgctgaac cttttttcac
    42961 tcggttttcc catcaacatg ctggcggggc tggtctgctt tgccaccctg ctctacaacc
    43021 tgccggatca ctatctgcat ctggcgaatt ttgtgttgca gcaactcgac gcgctgaaag
    43081 gtcactatgg cggatagcag cagcgaagaa aaaacagaga aaccctcggc gcaaaagctg
    43141 cgtaaagcgc gcgaagaggg gcaactgccg cgctcgaagg atatggggct ggcggcgagc
    43201 ctgtttgcgg catttgtggt gatctccagc agcttcccgt ggtatgccga ttttgtgcgc
    43261 gagagtttta tcagcgtgca ccagtatgca caggagatta ataacccaga ggtgatcggc
    43321 cagtttctgc gccatcacct gttaattctc ggcaagttta ttctcacctt gctgccgatg
    43381 cccgccgccg cgctactctc ctcactagtg cccggcggct ggctgtttct gcctaaaaaa
    43441 atcctcccgg atttcagcaa aatcagcccg ttaaaaggca ttgggcggct gttttccagt
    43501 gaacatctgg cggaaacggg aaagatgacg gtgaagtcgg tggtggtgct ggtgatgctg
    43561 tgggtaagcc tgcgcaataa ctttgcctcc tttctcggcc tgcaggcgct accgttcaaa
    43621 ctggcgatga atgagggttt atcgctctac gcctgcgtga tgcgcaactt tgtcatcctg
    43681 tttatcttct ttgcgttaat cgacgtgccg ctggcgaaag ccctgtttac caaagggctg
    43741 aagatgacca aacaggagct gaaagaagag tataaaaacc aggaaggcaa gccggaagtg
    43801 aaggcgcgcg tgcggcgtct gcaacggcag ttggcgatgg ggcaaattcg caaagtggtg
    43861 ccgaaagcca acgtggtgat caccaacccg acccactacg ccgtggcgtt gcagtatgac
    43921 tcgtcgcgcg ccgccgcgcc gtttgtggtg gcaaaaggca ccgatgaaat cgccctctat
    43981 attcgccagg tcgccgctga gaatcaggtg gaagtggtcg agttcccgcg actggcgcgc
    44041 tcggtttact acaccaccca ggtgaaccag caaattccgt ttcagcttta ccgggccatc
    44101 gcccacgttc tgacctacgt cctgcaaatg aaacactggc gcgagggtgc acagccgcgc
    44161 ccggccctga acagacatat ttccattccc aaagaggtgc ttaaactgga tggcgaaaac
    44221 aactaaatcg ttcctcgcgc tgctgcgcgg cggcaatctt ggcgtgccgc tggtgatcct
    44281 gtgtattctg gcgatggtta ttttgccgct gccgcctgcg ctgctggata ttctgttcac
    44341 cttcaacatt gtgctggcgg tgatggtgct gctggtggcg gtgtcggcga aacgaccgct
    44401 ggaattcagc ctgttcccga ccattttgct gatcaccacc ttaatgcgcc tgacgctcaa
    44461 cgtcgcctct acgcgcgtgg tgctgctgca cgggcatctc ggcgcggacg cggcgggtaa
    44521 ggtgattgag tcgtttggtc aggtggtgat cggcggcaac tttgttgtcg gctttgtggt
    44581 gtttatcatc ctgatgatca tcaactttat tgtcgtcacc aaaggggccg agcgtatttc
    44641 cgaggtttca gcccgcttta ccctggatgc gatgcccggt aagcagatgg cgattgacgc
    44701 cgatcttaat gccgggttga tcaatcaggc gcaggcacaa acccgacgta aagatgttgc
    44761 cagcgaggcc gatttctacg gcgcgatgga cggggcatcg aaatttgtgc gcggagatgc
    44821 tatcgccggg atgatgattc tggcgatcaa cctgatcggc ggcgtctgta tcgggatctt
    44881 caaatacaac ctgagcgccg acgccgcctt ccagcagtat gtgctgatga ccatcggcga
    44941 cgggctggtg gcgcaaatcc cttccctgct actctccacc gcggcggcga ttatcgtcac
    45001 ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt caccagttgc tggcaagccc
    45061 atcggtgctc tacaccgcta ccggaattat gttcgtactg gcggtagtgc ctggaatgcc
    45121 acatctgccg tttttgctgt tcagcgccct gcttggcttt accggctggc ggatgagcaa
    45181 acgcccgcag gcggcggaag cggaagagaa aagtctcgaa acgctgaccc gcaccatcac
    45241 cgaaaccagc gagcaacagg tcagttggga aaccattccg ctgatcgagc ccatcagctt
    45301 aagcctcggc tacaagctgg tggcgctggt ggacaaagcc cagggcaacc cgctcaccca
    45361 gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac ggcgtgctgc tgccggagat
    45421 ccgcattcgg gaaaacttcc gccttaagcc cagccagtac gctattttca tcaacggcat
    45481 taaggctgat gaagcggata ttccggcgga taaactgatg gcgctgccct ccagcgaaac
    45541 ctacggcgag atagacggcg tgctggggaa cgacccggcg tacgggatgc cggtgacctg
    45601 gatccagcct gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc
    45661 cagcgtgatt gctacccatg taaataagat tgtgcgcagc tatattcctg atttatttaa
    45721 ctatgacgat attacgcagt tgcataaccg tttggcgtcg atggcaccgc gcctggcgga
    45781 ggatttaagc gcggtgctca attacagcca gttgctgaaa gtgtaccggg cgctgctgac
    45841 cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc gtgctggtcg ccagtagcgc
    45901 ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc ctggcgctgc ggcgcagcat
    45961 tacccatccg ttcgttcgca agcaggagct gacggtgtat accctgaata atgagctgga
    46021 aaatctgctg actaacctag tgaatcaggc gcaacagggc ggtaaagtga tgctcgacag
    46081 cgtgccagtg gacccgaata tgctcaacca gttccagagt acgatgccac aggtgaaaga
    46141 gcagatgaaa gcggcgggga aagacccggt gctgctggtg ccaccgcagc tgcgcccttt
    46201 gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt
    46261 gccggatgag ctggagttga agattatggg ggcgttgagt tgatggggga ttcttttcca
    46321 tgctttgcag cacctgacac cattaattga cgtcacactg caaagagact acgatgacac
    46381 catacaatga cgtcatggat tgatagtatg attaaggtca gcgcgctagg ttctcgtcat
    46441 agaaaaacat tcgacgatgt aatgacggag ccgccaaaat ctggaattaa atgggatgat
    46501 gttgttgcac tcattaaagc tgtgggtgga acaataaaaa ataacaacgg ctccagacga
    46561 aaatttcaga ttggaacgac caaattcagc acacatgagc cacatccaaa aaatgtgatg
    46621 gataaaggcg cagtagcagg tcttaaggag tggtttgtta actgtgtagg ggtagattat
    46681 gaataaagca tcgaacatta tcacaattaa aggtcaacca gccgcagtaa catttgaagc
    46741 cgatatcaac gcatttcgcg gcaagttcct caatgtaaac ggttattgtg actttgtagc
    46801 cacaagtatt gaggcattat atcgtgaagg cgaaagcgcg ctggatgagt ggatggcgga
    46861 ttgtgaagag gacggtattg ccccttttcg tgaagaagaa gagcaaaaac gcctgacgct
    46921 tcgtgttcct catcacattg attcgcggct gaccatcgtc gccagacaac attccatttc
    46981 aaaaaaccag ctcattgttg atgtcttaga acgtgagttt tcagcccaga tgtaaacagg
    47041 tcggatgccg cgtggcatcc gaccttttgc cagttcgtca atattaccca tttacaaaat
    47101 cgtctcctcc gtcaaaatcc gccgcgcgcc taaaaagtgc tcctgccaga aaggttcagc
    47161 taactgactt acccgaatgg tttccccggt gcgcggtgac tcaataaact gaccgtcgcc
    47221 cagatacacc cccatatggt cggcaatctc gcggctgtgg atatggaaaa atagcaaatc
    47281 tccccggcgc aggtcgttat tcgccacaat ggttgcccgg cgatagtgat acatctcatt
    47341 agccgtgcgt gggagtttcg cctcaaggat cttgttgtag gcataaaaaa ctaacccgct
    47401 acagtcgaag ccttgatcgg gccgcgtacc gccccagacg taaggcttgc caagctgttg
    47461 ctccagacgg tgaatcgcca cttcggtgat gttatgcaga tggtcgctgc ttaagaaatg
    47521 gttgttttcc gccagcgcct gccagcgtct gtcactcgcc ttgagcggcg caggaaacca
    47581 ttctggatgc tgttttatct gttcgcggtt gtgctggcgt agcgcccttc tgctttcggc
    47641 attgccttcc acaatatagc tggcctgttt tttcagatgg gtttggtact gttttaataa
    47701 acgcgcacga ttttgcatcc gctggcgggc ggcgtagctg aaagtgatat gtgatgtggt
    47761 ggcgctggca aatgaagaaa acggcaagca aagcaacagt aatcccgaca acacaaaaga
    47821 gggaatcgac ggcaaggaca tactcaataa tggctgatca tgcattgatg ttgcagtgta
    47881 tcccggcccg ta
//
LOCUS       DAZUFB010000027        47167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZUFB010000027 DAZUFB010000000
VERSION     DAZUFB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.027"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..1049
                     /gene="focA"
                     /locus_tag="ACHY3M_001766"
     CDS             192..1049
                     /gene="focA"
                     /locus_tag="ACHY3M_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFS4316698.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1104..3386
                     /gene="pflB"
                     /locus_tag="ACHY3M_001767"
     CDS             1104..3386
                     /gene="pflB"
                     /locus_tag="ACHY3M_001767"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4316699.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3578..4318
                     /gene="pflA"
                     /locus_tag="ACHY3M_001768"
     CDS             3578..4318
                     /gene="pflA"
                     /locus_tag="ACHY3M_001768"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFS4316700.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4400..4990)
                     /gene="ycaK"
                     /locus_tag="ACHY3M_001769"
     CDS             complement(4400..4990)
                     /gene="ycaK"
                     /locus_tag="ACHY3M_001769"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFS4316701.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5090..5998
                     /gene="ycaN"
                     /locus_tag="ACHY3M_001770"
     CDS             5090..5998
                     /gene="ycaN"
                     /locus_tag="ACHY3M_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4316702.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5999..7429)
                     /gene="ycaM"
                     /locus_tag="ACHY3M_001771"
     CDS             complement(5999..7429)
                     /gene="ycaM"
                     /locus_tag="ACHY3M_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4316703.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7637..8785)
                     /gene="ycaD"
                     /locus_tag="ACHY3M_001772"
     CDS             complement(7637..8785)
                     /gene="ycaD"
                     /locus_tag="ACHY3M_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4316704.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9100..9726
                     /gene="ycaC"
                     /locus_tag="ACHY3M_001773"
     CDS             9100..9726
                     /gene="ycaC"
                     /locus_tag="ACHY3M_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFS4316705.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9762..10625)
                     /gene="dmsC"
                     /locus_tag="ACHY3M_001774"
     CDS             complement(9762..10625)
                     /gene="dmsC"
                     /locus_tag="ACHY3M_001774"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFS4316706.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPVLRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10627..11244)
                     /gene="dmsB"
                     /locus_tag="ACHY3M_001775"
     CDS             complement(10627..11244)
                     /gene="dmsB"
                     /locus_tag="ACHY3M_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFS4316707.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11255..13699)
                     /gene="dmsA"
                     /locus_tag="ACHY3M_001776"
     CDS             complement(11255..13699)
                     /gene="dmsA"
                     /locus_tag="ACHY3M_001776"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFS4316708.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNGRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13938..15230)
                     /gene="serS"
                     /locus_tag="ACHY3M_001777"
     CDS             complement(13938..15230)
                     /gene="serS"
                     /locus_tag="ACHY3M_001777"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4316709.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15321..16664)
                     /gene="rarA"
                     /locus_tag="ACHY3M_001778"
                     /gene_synonym="mgsA"
     CDS             complement(15321..16664)
                     /gene="rarA"
                     /locus_tag="ACHY3M_001778"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFS4316710.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16675..17286)
                     /gene="lolA"
                     /locus_tag="ACHY3M_001779"
     CDS             complement(16675..17286)
                     /gene="lolA"
                     /locus_tag="ACHY3M_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFS4316711.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17441..21547)
                     /gene="ftsK"
                     /locus_tag="ACHY3M_001780"
     CDS             complement(17441..21547)
                     /gene="ftsK"
                     /locus_tag="ACHY3M_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4316712.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAASTTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQQQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21682..22176)
                     /gene="lrp"
                     /locus_tag="ACHY3M_001781"
     CDS             complement(21682..22176)
                     /gene="lrp"
                     /locus_tag="ACHY3M_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFS4316713.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22720..23685
                     /gene="trxB"
                     /locus_tag="ACHY3M_001782"
     CDS             22720..23685
                     /gene="trxB"
                     /locus_tag="ACHY3M_001782"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4316714.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23808..25574
                     /gene="cydD"
                     /locus_tag="ACHY3M_001783"
     CDS             23808..25574
                     /gene="cydD"
                     /locus_tag="ACHY3M_001783"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFS4316715.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25575..27296
                     /gene="cydC"
                     /locus_tag="ACHY3M_001784"
     CDS             25575..27296
                     /gene="cydC"
                     /locus_tag="ACHY3M_001784"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFS4316716.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27338..28042
                     /gene="aat"
                     /locus_tag="ACHY3M_001785"
     CDS             27338..28042
                     /gene="aat"
                     /locus_tag="ACHY3M_001785"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFS4316717.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28327..28545
                     /gene="infA"
                     /locus_tag="ACHY3M_001786"
     CDS             28327..28545
                     /gene="infA"
                     /locus_tag="ACHY3M_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4316718.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28799..28886
                     /locus_tag="ACHY3M_001787"
     tRNA            28799..28886
                     /locus_tag="ACHY3M_001787"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28833..28835,aa:Ser,seq:gga)
     gene            complement(29230..31506)
                     /gene="clpA"
                     /locus_tag="ACHY3M_001788"
     CDS             complement(29230..31506)
                     /gene="clpA"
                     /locus_tag="ACHY3M_001788"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFS4316719.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(31537..31857)
                     /gene="clpS"
                     /locus_tag="ACHY3M_001789"
     CDS             complement(31537..31857)
                     /gene="clpS"
                     /locus_tag="ACHY3M_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFS4316720.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(31956..32030)
                     /gene="yljB"
                     /locus_tag="ACHY3M_001790"
     CDS             complement(31956..32030)
                     /gene="yljB"
                     /locus_tag="ACHY3M_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFS4316721.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            32180..32404
                     /gene="cspD"
                     /locus_tag="ACHY3M_001791"
     CDS             32180..32404
                     /gene="cspD"
                     /locus_tag="ACHY3M_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFS4316722.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(32477..34423)
                     /gene="macB"
                     /locus_tag="ACHY3M_001792"
     CDS             complement(32477..34423)
                     /gene="macB"
                     /locus_tag="ACHY3M_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFS4316723.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(34420..35535)
                     /gene="macA"
                     /locus_tag="ACHY3M_001793"
     CDS             complement(34420..35535)
                     /gene="macA"
                     /locus_tag="ACHY3M_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFS4316724.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            35686..36642
                     /gene="ybjX"
                     /locus_tag="ACHY3M_001794"
     CDS             35686..36642
                     /gene="ybjX"
                     /locus_tag="ACHY3M_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFS4316725.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(36639..38297)
                     /gene="ybjD"
                     /locus_tag="ACHY3M_001795"
     CDS             complement(36639..38297)
                     /gene="ybjD"
                     /locus_tag="ACHY3M_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS4316726.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            38723..39418
                     /gene="aqpZ"
                     /locus_tag="ACHY3M_001796"
     CDS             38723..39418
                     /gene="aqpZ"
                     /locus_tag="ACHY3M_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFS4316727.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            39913..40812
                     /gene="lysO"
                     /locus_tag="ACHY3M_001797"
     CDS             39913..40812
                     /gene="lysO"
                     /locus_tag="ACHY3M_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFS4316728.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            40956..42608
                     /gene="hcp"
                     /locus_tag="ACHY3M_001798"
                     /gene_synonym="priS"
     CDS             40956..42608
                     /gene="hcp"
                     /locus_tag="ACHY3M_001798"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFS4316729.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            42620..43588
                     /gene="hcr"
                     /locus_tag="ACHY3M_001799"
     CDS             42620..43588
                     /gene="hcr"
                     /locus_tag="ACHY3M_001799"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFS4316730.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            43721..45439
                     /gene="poxB"
                     /locus_tag="ACHY3M_001800"
     CDS             43721..45439
                     /gene="poxB"
                     /locus_tag="ACHY3M_001800"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFS4316731.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHTPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            45476..46477
                     /gene="ltaE"
                     /locus_tag="ACHY3M_001801"
     CDS             45476..46477
                     /gene="ltaE"
                     /locus_tag="ACHY3M_001801"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFS4316732.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            46488..>47167
                     /locus_tag="ACHY3M_001802"
     CDS             46488..>47167
                     /locus_tag="ACHY3M_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS4316733.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
BASE COUNT        11314 a        12463 c        11709 g        11681 t
ORIGIN      
        1 gaaaaaattc aactcatttg ttaattttta aaatttattt ttatttggat aatcaaatat
       61 ttactccgta tttgcataaa aaccatgcga gttacgggcc tataagccag gcgagatatg
      121 atctatatca atttctcatc tataatgctt tgttagtatc tcgtcgccga cttaataaag
      181 agagagttag tgtgaaagct gacaaccctt ttgatctttt acttcctgct gcaatggcca
      241 aagtggccga agaggcgggt gtctataaag caacgaaaca tccgcttaag actttctatc
      301 tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt cttctatatc acagcaacca
      361 ctggcacagg cacaatgccc ttcggcatgg caaaactggt tggcggcatt tgcttctctc
      421 tggggctgat tctttgtgtt gtctgcggag ccgatctctt tacttccaca gtgttgattg
      481 ttgttgctaa ggcgagtggg cgcatcacct ggggtcagtt ggcgaaaaac tggctaaatg
      541 tctattttgg caacctggtc ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg
      601 agtatatgac cgcaaatggt caatggggac taaacgtcct acaaaccgcc gaccacaaag
      661 tgcaccatac ttttattgag gccgtctgcc ttggtatcct ggcaaacctg atggtatgtc
      721 tggcagtatg gatgagttat tctggccgta gcctgatgga caaagcgttc attatggtgc
      781 tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag tatcgcaaac atgtttatga
      841 tcccgatggg tattgtaatc cgcgacttcg catctccgga attctggacc gctgtcggtt
      901 ctgcaccgga aaatttttct cacctgaccg tgatgaactt catcactgat aacctgattc
      961 cggttacgat cggtaatatt atcggcggtg gtttgttggt tgggttgaca tactgggtca
     1021 tttacctgcg tgaaaacgat caccattaat ggttgtcgaa gtacgcagta aataaaaaat
     1081 ccacttaaga aggtaggtgt tacatgtccg agcttaatga aaagttagcc acagcctggg
     1141 aaggttttac caaaggtgac tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa
     1201 actacactcc gtacgagggt gacgagtcct tcctggctgg cgctactgaa gcgaccacca
     1261 ccctgtggga caaagtaatg gaaggcgtta aactggaaaa ccgcactcac gcgccagttg
     1321 actttgacac cgctgttgct tccaccatca cctctcacga cgctggctac atcaacaagc
     1381 agcttgagaa aatcgttggt ctgcagactg aagctccgct gaaacgtgct cttatcccgt
     1441 tcggtggtat caaaatgatc gaaggttcct gcaaagcgta caaccgcgaa ctggatccga
     1501 tgatcaaaaa aatcttcact gaataccgta aaactcacaa ccagggcgtg ttcgacgttt
     1561 acactccgga catcctgcgt tgccgtaaat ctggtgttct gaccggtctg ccagatgcat
     1621 atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc gctgtacggt atcgactacc
     1681 tgatgaaaga caaactggca cagttcactt ctctgcaggc tgatctggaa aacggcgtaa
     1741 acctggaaca gactatccgt ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc
     1801 agatgaaaga aatggctgcg aaatacggct acgacatctc tggtccggct accaacgctc
     1861 aggaagctat ccagtggact tacttcggct acctggctgc tgttaagtct cagaacggtg
     1921 ctgcaatgtc cttcggtcgt acctccacct tcctggatgt gtacatcgaa cgtgacctga
     1981 aagctggcaa gatcaccgaa caagaagcgc aggaaatggt tgaccacctg gtcatgaaac
     2041 tgcgtatggt tcgcttcctg cgtactccgg aatacgatga actgttctct ggcgacccga
     2101 tctgggcaac cgaatctatc ggtggtatgg gcctcgacgg tcgtaccctg gttaccaaaa
     2161 acagcttccg tttcctgaac accctgtaca ccatgggtcc gtctccggaa ccgaacatga
     2221 ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa attcgccgct aaagtgtcca
     2281 tcgacacctc ttctctgcag tatgagaacg atgacctgat gcgtccggac ttcaacaacg
     2341 atgactacgc tattgcttgc tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct
     2401 tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg
     2461 aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat caaaggcgat gtcctgaact
     2521 atgatgaagt gatggagcgc atggatcact tcatggactg gctggctaaa cagtacatca
     2581 ctgcactgaa catcatccac tacatgcacg acaagtacag ctacgaagcc tctctgatgg
     2641 cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg
     2701 ctgctgactc cctgtctgca atcaaatatg cgaaagttaa accgattcgt gacgaagacg
     2761 gtctggctat cgacttcgaa atcgaaggcg aatacccgca gtttggtaac aatgatccgc
     2821 gtgtagatga cctggctgtt gacctggtag aacgtttcat gaagaaaatt cagaaactgc
     2881 acacctaccg tgacgctatc ccgactcagt ctgttctgac catcacttct aacgttgtgt
     2941 atggtaagaa aactggtaac accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg
     3001 gtgctaaccc gatgcacggt cgtgaccaga aaggtgctgt agcgtctctg acttccgttg
     3061 ctaaactgcc gtttgcttac gctaaagatg gtatctccta caccttctct atcgttccga
     3121 acgcactggg taaagacgac gaagttcgta agaccaacct ggctggtctg atggatggtt
     3181 acttccacca cgaagcatcc atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc
     3241 gtgaaatgct gctcgacgcg atggaaaacc cggaaaaata tccgcagctg accatccgtg
     3301 tatctggcta cgcagtacgt ttcaactcgc tgactaaaga acagcagcag gacgttatta
     3361 ctcgtacctt cactcaatct atgtaattag atttgactga aatcgtacag taaaaagcgt
     3421 acaataaagg ctccacgaaa gtggggcctt ttttagcacg agagcctttt ttgtcagcta
     3481 tctatacttt aaggtgactg ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac
     3541 acggcctcag atgggccaca tctggagaaa caccgcaatg tcagttattg gtcgcattca
     3601 ctcctttgaa tcctgtggaa ccgtagacgg cccaggtatt cgctttatca cctttttcca
     3661 gggctgcctg atgcgctgcc tgtattgtca taaccgcgac acctgggata cgcatggcgg
     3721 taaagaagtt accgttgaag atttaatgaa agaggtggtg acctatcgcc actttatgaa
     3781 cgcttccggc ggcggcgtta ccgcatccgg cggtgaggca atcctacaag ctgagtttgt
     3841 tcgtgactgg ttccgcgcct gcaaaaaaga aggcattcat acctgtctgg acaccaacgg
     3901 ttttgttcgt cgttacgatc cggtgattga tgaactgctg gaagtaaccg acctggtaat
     3961 gctcgatctc aaacagatga acgacgagat ccaccaaaat ctggttggag tttccaacca
     4021 ccgcacgctg gagttcgcca aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta
     4081 cgttgttgtc ccaggctggt ctgacgatga cgattcagcg catcgcctcg gtgaatttac
     4141 ccgtgatatg ggcaacgttg agaaaatcga actcctcccc taccacgagc tgggcaaaca
     4201 caaatgggtg gcaatgggtg aagagtacaa actcgacggt gttaaaccac ctaagaaaga
     4261 gaccatggaa cgcgtgaaag gcattcttga gcagtacggt cataaggtaa tgttctaatt
     4321 tccccgtaaa gcggcaactt tattgagttg ccgctttttt attccgcgct tcattctgct
     4381 acagcgtttt taacacattt tatagtgcat ctaccatatc ccgtacctga gataataacg
     4441 actgataata gctcgcatga agttcttgcc catcgaatac cactgtatta cacaagaaag
     4501 tgacatcggc atcttcaata ccaagataac tgcacatatt tttcaaataa tcgcttatat
     4561 ttttttccca gcccatctgg acaaatgatt ctttgtctcc tccaaccagc gccacccaac
     4621 gaactttatt gaatggtaat ttgtgcccat ctccataagc cagcccatta ttccatactc
     4681 tgtcaatata tccttttagc attgccggga agctatacca ccagagagga aaaaccacca
     4741 ctaacgtgtc atgttcaagc agctctgaat aaagctgatg aacctctgga gaataacgtt
     4801 tatccatatt cttccagtct ggttcatctt ccggcgtcat cactgggttg aaattacgcc
     4861 gatataaatc gagttccgtc acctgtattt tccgctccat tgcccgctga tggatcgcat
     4921 cagcaatatt tgcggtcaat gaatcatgac gaggatgggc ccataccaaa taaatacgtt
     4981 cagactgcat aattttacct ccacgttaag ttctggagcg cactttacgc aggcggcata
     5041 atcctgtaaa cgtgatgatt caggatgagt taatgaagga ttttcactaa tgcggatgaa
     5101 tatgtctgac tttgccactt tctttgccgt cgcccgtaat caaagctttc gtgcagcggg
     5161 cgatgagtta ggcttatcct cgtccgccat tagccatagt attaaaacac tggaacaacg
     5221 tcttaaaatt cgcttattca atcggacaac ccgaagcgta tcgttaacgg aagcgggttc
     5281 taatctctat gaacgattgc gcccagcttt cgacgaaatt cagatcatgt tagatgagat
     5341 gaatgatttt cgtctgacac cgacaggaac gctcaaaatc aatgccgccc gggtagctgc
     5401 acgtatattt ttaatgcctt tactggttgg tttcacacgt gaatatcctg atattaaagt
     5461 cgagctcaca actgacgatt ctcttgtcga tatcgtccaa cagggctttg acgctggcgt
     5521 tcgacttagt ggcatcgtcg aaaaagatat gatcagcgtg gcgatcggcc caccagtgaa
     5581 attatgtgta gcagcaacgc cagagtactt tgcaagatat ggcaaacccc gccatcctca
     5641 tgatctgctt aatcatcaat gtgttgtttt tcgttatccc agcggcaaac ctttccactg
     5701 gcaatttgca aaggagctgg aaattgctgt cgcaggaaat atcattctgg atgatgttga
     5761 tgctgaactg gaggccgttt tgatgggtgc tggtatcggg tatctgctct atgaacagat
     5821 caaggagtac cttgatactg gtcgtttaga atgtgtactg ggagactgga gcacagagcg
     5881 tccaggcttc cagatctatt atccaaaccg ccagtatatg tcctgcggct tgcgcgcatt
     5941 tctcgattat gtaaaaacgg gtcagatctg ccagagtcag cgtcaccgac cacaataatt
     6001 atttactatt cgctttacgc gccagcagcg ggaaaatcag tcctaatcct accagcacaa
     6061 acggcgttgc aacattcagc gccaactgga aggtccactc tgcagtgaag gcttccattt
     6121 tcgggaagat ccccgtcaaa caggcaaagg cggtaaaggc aaaacaccaa atcccgacgg
     6181 tcattgccag aggcttatta cgaataaaga catattctgg tttatatttc tgcgccaggc
     6241 gaacgactgc aataaatgca acaaataccc ataaataacg cagcggcatc actaccgagt
     6301 taagattcaa cagccattta tagagattgt tcatatcacc gatgccgaga gtcggcagca
     6361 taatcagaat tgccaccagc accagggtca gaaaatagcc attaacgggc gtaccagaag
     6421 cgttggtacg acataaactg gctggaatat atttgctgtc agcatcacct aatagcactt
     6481 taagcggggc atcaatcgag aataccagcg cagctacttg tcccagggta ttcgcaatgg
     6541 cgtaaatcac cattaaagta ttacccatgt tgtaatactc gcccagcttc tgaaaggcgt
     6601 aatattgacc gttagtcatt aagtcatccg ggatattacg cgagtcaaac atcatcccca
     6661 tcgccagtga gcccagaatg gcacaaaccg caaccatcac cgccaggcat agcatccctt
     6721 ttggaaattc ttttcctggg ttgcgcgttt gattaacgta aggagaaatc ttctctgctc
     6781 cgccaaccgc gaaaaccagc attgaaatag tggtaatgta ggtaaagtcg atatgaggaa
     6841 tgaacgtttc ccaggtaatg tttgtggtcg caatatgcac ttcagtaatc gcaggcgcgg
     6901 ttaccgccat cgccacatac aggagcgaca taacaaacat cgcaatccct gccacagaac
     6961 cgacgatttt cagcgatttc ataccgcgtg aagcaaccca cataaagaag ataaacagca
     7021 ccagcgttaa cccctgtaac gctacgactg aatattcttt gattagcgaa ccgtcgcctt
     7081 tcatcgccca accgagcgca atcagaattg cctggggttt ttgtgccaga tagggaatat
     7141 gcaccaccca gtaggtccac gcggcgagat aagccagtcc gggtcccatc gtgtgtttaa
     7201 tccaggtact gaccccgccc ttcccatctt tgaaggtcga gcctaactga ccaacaatta
     7261 gcgcataagg tgtgaaatag agtgcaaaga taaacaccca tgaaaaaaca accaccagcc
     7321 cctggttggc atagttatta acaacgttgc caaaacccca gacagtgata aaagacatca
     7381 gcgcaatgtt gtaccatcgc aactgttttt cctgaacatt cccagccata gacaaatcct
     7441 tcgccaaaat taaaggggca tctgtaaaac gagggattat gcgcggcaaa taagaagtgc
     7501 ggaaatagcg aaatgcaaaa ttataacctg ctgcacattt tatttttaaa cgaataaaat
     7561 ttggaataaa gcaagtcaga tagaagaaaa aaaagcagag tgtgaaacag taagttcaca
     7621 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
     7681 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
     7741 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
     7801 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
     7861 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
     7921 ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
     7981 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcggccat tgtccaagga
     8041 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
     8101 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
     8161 agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt gacgtcgaat
     8221 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
     8281 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     8341 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     8401 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     8461 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     8521 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     8581 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     8641 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     8701 cgagtgtatt taacaccgcg atcgccagag tcaacaaaag caggccagac agcaaaagca
     8761 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     8821 catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgcatat cgcagcggcg
     8881 gtattatcga tttatattac tcattcataa cgctaatgct gaagagatga ttaatctcat
     8941 ctgattgttt ttattgatct aacacaaatt tcatacgctt cgttcagtcg aaaaatttca
     9001 tggtcacagc aaactcatat ttcccgtcta tgctttcaga gcaatgcatc acgtctgacc
     9061 gatttcagac gtaccactga ctctcgaaag gagaagagga tgaccaaacc gtatgttcgt
     9121 cttgataaaa atgatgctgc cgttttgctt gttgatcatc aggctggttt actttccctt
     9181 gtacgggata tcgaacccga taagtttaaa aataacgtgc tggcgctggg tgacctggct
     9241 aagtatttca acttacctac tattcttacc accagttttg aaaccggtcc taacggtcca
     9301 ttagttccag aactgaaagc acaattcccc gatgcacctt acattgctcg cccgggaaat
     9361 attaacgcct gggataacga agattttgta aaagcagtca aagcgacagg taaaaaacag
     9421 ttaattattg ccggtgtggt aaccgaagtt tgcgtggcat tcccggcgct ttcagccatt
     9481 gaagaagggt ttgatgtctt tgttgtgact gacgcgtcag ggacctttaa tgaaattacc
     9541 cggcattcgg catgggatcg catgtcgcaa gcaggtgcgc aattgatgac atggttcggc
     9601 gtggcgtgcg agctgcaccg tgactggcgt aatgatattg aaggactggc gacgttgttc
     9661 tctaaccata ttcctgatta tcgtaatttg atgaccagtt atgacacgtt aacgaagcag
     9721 aaataaaaga gaaccggggc cgaaaccccg gttgttactg attagcttgc gacggccatc
     9781 cccacggtca tatgcaaacc atagaatacg ccgcgaccga ttaattcccc tgccagaagc
     9841 agaatgaatg agacggatag tagcggaacc gcaggctgat aaccttttag ctgtggtgca
     9901 atccacaagc acagggcaac ggccaaaagc acgatccgcc aggacatcag cgcaccatag
     9961 tccggcacca gcgctgcggc ctgctgcaca gaactatgaa tagttgccag ctctgtgccc
    10021 tgcatcactg acatcacgcc actcaccacc agtgccagta cagaaatggc tggcagcaga
    10081 cgcatcgccc agccatcgac gccagccaga ctcaacaaca ggtaacccag taacgggccg
    10141 cccataaaca tcgtcaggaa gaagcccatc ggcgtccaga tgctgtacca ggtcggaacg
    10201 gtatcaatgc tgttatacac acgcaccatc atccagacaa agatgacgcc aagaaccatc
    10261 gtcactatca gccacagcgt acgcaatacc ggtgacagct ttttcagcat tgccagcaac
    10321 cagccgatgc cgcctacggc aaaaaagatc gaaccgctgg cgatttcgtt actgagtgct
    10381 gaagccccta cccggttgag cgagttaaaa gcgcgcattg gtgaaccaag atggagcata
    10441 gaggcgataa agccaatgcc catcagcacc cataaaccaa acatgcaggc gataacacgc
    10501 tgctgggctt ctgctcgcag gtcgcctttg agcagcgcca aagccagaac gataaaacca
    10561 cctgctacac attgcccgaa gaccgtgaag atcatcagcg gccattcatg ccatccactt
    10621 cccatctcac acctccttcg ggtttgccag atagccagtg gtatccccgg tcgggcggct
    10681 attggcattg ggtttgatca caatattcgg tttggtaaag tgagctcgcg gcaacggcgc
    10741 aacggccgcc agatcgccat gttttttacg cagctcgtcg atagggccga aatccagcgc
    10801 ccgcagcgga caagattcaa cacagatcgg ctttttaccc tccgcaacac ggtcataaca
    10861 accatcgcat ttggtcatat ggcctttcgt ttcgttatat tgcggtgcgc catacgggca
    10921 agccatatgg cagtagcggc agccaatgca cacatcttca tcgaccacaa caaaaccatc
    10981 ttcacgttta tgcatcgcac cgctcgggca gactttagta caagccggat cttcgcagtg
    11041 gttacatgaa atcgacagat agtaggcaaa cacgttctgg tgccagacac cgttatcttc
    11101 ctgccagtcg ccgccagcat attcataaat gcggcggaag ctgacttctg gcgtcaaatc
    11161 tttgtagtct ttacaggcca gctcgcaggt tttgcaaccg gtgcaacggc tggaatcaat
    11221 aaaaaatcca tactgggttg tcatcggtta ctccttacac cttttcaacc tgaacaaggt
    11281 ttgtatgtga cggattcccc ttagcgagag gagacggacg ttgagtggtc agtacgttga
    11341 tacaaccgcc cttatcgaca cgttttgtat ccgggtcata ccaggcacct tcacccagtg
    11401 cgaccacacc cggcatcatt cgtggcgtca ctttcgcctc aatatgaacc tcaccacgac
    11461 cgttaaagat cctgacttta tcgccgttgt tgataccgcg tttttgcgca tcaagcgggt
    11521 tgatccacat ttcctgacgg caagctgctt tcagcacatc aacgttgccg taagttgagt
    11581 gaacgcgaga tttatagtgg aaacccgtaa gctgcagcgg atactgtttg ttcagcggat
    11641 cctgataact ttcaaagccc ggcgtgtaga tcggcagtgg atcgatcaca tcgccttctg
    11701 gcaattccca ggtagcggca atgtcagcca gcgcctgcga ataaatctca attttgcccg
    11761 atggcgtagt cagtgggttt gcctgcggat cttcacggaa ggctttataa gcaacgtgat
    11821 gcccttgtgg gtcgcgcttt ttaaagatcc cctgcttgcg gaactcttca aacgttggca
    11881 gttcaggaat cgcttcccgc gactgggcat acagatgccg catccactct tcctgggtac
    11941 ggccttcagt aaactgttgc tcaacgccaa gacgttttgc cagttcgctg gtcatttcat
    12001 agatggtctt acattcaaag cgcggtttaa tcacctgatc gttgaaaatc acgtaagaca
    12061 tattcccgca ggatgcatcc agtgcaaagt ccatctgttc ggaagcggtg cagtcaggca
    12121 gcaggatgtc agcatatttc gccgatgagg tcatgtggca gtcgataacc acaatcagct
    12181 cgcacttctt atcatcctga aggatttcat gggtacggtt gatttcagaa tgctggttaa
    12241 tcaggcagtt accggcatag ttccagatca ttttgatcgg cacatccagc ttatctttcc
    12301 cgcgtacacc atcacgcagc gccgtcattt ccgggccacg ttcaatggca tcggtccaca
    12361 taaacatcga aatgctggtc tggatcgggt tttccaaggt cggcatacgg acaaacggta
    12421 agctgtatga accttcacgc gcgccgctgt tgcctccgtt aataccaacg ttaccggtca
    12481 gaatcgccag catcgagata gcacgggttg cgatttcacc gttagcgtga cgctgcgggc
    12541 cccatccctg gctgataaac gccggtttgg tactgccgat ttcacgagcc agtttgatga
    12601 ttttgtctgc cggaacaccg gtgatttgtg aggcccattc cggtgtttta gccacgccat
    12661 ctggcccttc acccagaata taagctttat agtggccatt tttcggcgca ctggctggca
    12721 gggttttctc atcgtagcca acgcaatatt tatcgaggaa tgcctgatcc accaggtttt
    12781 cagtgatcat gacgtacgcc agaccgttaa ccagtgcggc atctgttccc ggacgaatag
    12841 ggatccactc atcttcgcgc ccggcaccgg tgtcggtata gcgcggatca atgatgatca
    12901 tgcgggcatt agatttctgg cgtgcctgtt caagatagta agtcacccca ccgccactca
    12961 ttcgcgtttc gccagggtta ttaccaaaca gcactaccag cttactgttt tcgatatccg
    13021 acgggctgtt gccatctgcc cagccgccgt aggtatagtt caaaccttcc gcaatttgcg
    13081 cggaggagta gtcgccgtaa tggttcagat agccgccgca gcagttcatc agccgcgcga
    13141 ccagggtatt tcccggcggc caggagcggg tcatggtgcc gcccagcgta ccggtgccat
    13201 agttcagata gatagactcg ttgccgtact ctttgatcag gcgctgcata ttggtcgcga
    13261 tgatgtcgta ggcttcttcc cagctaatgc gctcgaattt gccttcaccg cgcgccccga
    13321 ctcgtttcat cggatatttc aggcggtccg gattgtagac acggcgacgc atggaacgcc
    13381 cacgcaggca ggcgcgaacc tggtgcaggc cgtcgtaatt gtcatcgccg gtattgtccg
    13441 tttcgacata tttgatttca ccgtccacga cgtgcatacg tagcgggcag cgactaccac
    13501 agttaactgt acaggcgctc cagataacct tttcgtctga ttttgttgga atggcgctat
    13561 cgacagcgtg cgcaatccga ctaaaaggta atgttaatgc gctgctggcc attgccaggc
    13621 cgccgatcgc tgtcgttttt accaaaccac ggcgactcac ctcagcagcc aataccgcat
    13681 cagggatttt cgttttcata atggctcact caagcttgct cacgattgat tccagtaaca
    13741 aaccgtattg tgtaattatt tctatagcag ctcgctatat aggcttgtat acatcgaata
    13801 atttgaaaat caaagagata ggtagatttt cactcgaaag tagtattacg tgtaagtgag
    13861 gagtaattat tgttcggtat caaagggaga caaaaaaagc gcccgcaggc gctttttaga
    13921 ttcagaaaaa ttgggtatta gccaatatat tccagtccgt tcatatacgg acgcagaact
    13981 tctggtactt caatacgacc atcagcctgc tgatagtttt ccattactgc aaccagcgta
    14041 cgaccaacag ccagtccaga accgttcagg gtatgaacca gacgggtttt cttgtccgac
    14101 ttgctgcggc agcgtgcctg catacgacgc gcctggaaat cccaaacgtt ggagcaggaa
    14161 gagatctcac ggtaggtgtt ctgtgccggg atccatactt ccaggtcgta agttttgcaa
    14221 gcgccaaagc ccatgtcgcc agtgcaaagg atgattttac ggtacggcag gcccaacaac
    14281 tgcaggactt tttctgcatg accagtcatc tcttccagcg ccgccattga gtcttccggg
    14341 cgcacgatct gcaccatttc aactttgtcg aactggtgca tacggatcag accacgggtg
    14401 tcacgaccat atgaaccggc ttcagaacgg aagcatgggg tgtgggcggt catcttaatt
    14461 ggcagatcat cttcatcgat gatttcaccg cgcaccaggt tagtcagcgg aacttctgcc
    14521 gttgggatca gcgcatagtt actggtgtct gcttcttctt ccagcggacg agtatgaaac
    14581 agatcgccag caaatttcgg cagctgaccc gtaccgtaca gcgtgtcctg gttaaccagg
    14641 tacggaacat agttctcact gtagccatgc tgttcggtat gcagatccag cataaactgc
    14701 gacagtgcgc ggtgcatgcg agcaatctgc cctttcatta ccacaaagcg ggaaccagtc
    14761 agcttaactg cagctgcaaa gtcgaggcca gagtgcattt cacccagcgt cacatggtca
    14821 cgaacttcaa agtcaaactc acgcggggtg ccccagcggc tgacttcgac gttgtcattt
    14881 tcgtctttac ctaccggcac ttcatctgca ggcaggttag ggatggtcag cgcgatatcg
    14941 cgaatttcag cctgtaaagc atccagctcg gcttttgctg catccagctc ttcgcccagt
    15001 ttgttcactt ccagacgtaa aggctcgata tcttccccgc gcgctttcgc ctggccaatg
    15061 gatttcgatc gggagttacg ctccgcttgc aggttttccg ttttgacctg caatacttta
    15121 cgacgctctt caagagcgcc cagcttatct acatccagct taaagccccg gcgtgccagt
    15181 ttttcagcga ctgcgtctgg ctcattacgc agcagattgg gatcgagcat gcttatcctg
    15241 tgcttatcga attaaatggg agatgtggcc acagcctgcg accacaggga tacagtaaca
    15301 acattaccgc aacgataaca ttaacggtag cgttttatgg ggctattttg atcctgttca
    15361 gccagccagg cgagcttttc gccaatcttg ccttcaaggc ccctgtttgt cgggaaataa
    15421 tagcgtgttt gtgctatttc cggcgggaag taaacctcac cggcagcata agcgtttgct
    15481 tcatcatgag cgtaacgata ttcctgcccg tagcccattt ccttcattaa tttcgtcggc
    15541 gcattacgca aatgaaccgg cacgtcataa tccggacgtt cgcgagcatc cgccagcgca
    15601 gctttaaacg cagtgtagac agcgttgctt tttggcgcgc aggccaggta aacaatcgcc
    15661 tgagcaatgg cgcgttcacc ttccgccggg ccaacgcgag taaagcaatc ccaggccgca
    15721 attgccacct gcatcgcccg tgggtcggca ttgccgacat cttcggaagc aattgccagg
    15781 caacgacgcg cgacatataa cggatcgcca ccagcggtaa taattcgcgc ataccagtag
    15841 agcgccgcat cgggtgcgct accacgtacc gacttatgca gtgcggaaat gagatcgtaa
    15901 aagcgatcgc ctttgttatc aaagcgggcg cttcgctcac cggcgatttc ggtcaataat
    15961 tcaggcttca ggacccgctt accgctatca ttgacttcgg ccatatccgc catcatttcc
    16021 agcgtattta acgcccggcg cgcgtcgcca ttcaccagtt cagcaatggc gcgtcgtgtt
    16081 tcatctggca gaacaatatc ctgaccacca taaccacggg tcttgtcttc catcgcctga
    16141 gttagtactt gctcaatatc ctctgtaccc agggatttca acagatagac acgggcacgg
    16201 gaaagcagtg ccgaattaag ctcaaacgac gggttttcag tggttgcgcc aataaaagtg
    16261 atggtgccgt cttcaatatg tggcagaaat gcatcctgct ggcttttgtt gaatcggtgc
    16321 acttcgtcaa caaaaagaat agtgcggcga cctgcattgc ggttttgccg ggcgcgctcg
    16381 atcgcctcgc gaatctcttt cacgccagag gtgacggcag aaatacgttc cacatcagcg
    16441 ttcgcatagc gggcaatcac ttcagcgagg gttgttttgc cggtacccgg tggcccccag
    16501 aggatcatag aatgtaaatg cccggcttcg atagcgcgcg gcaacggctt ccccgcagcc
    16561 agcaaatgtt gctggccgat atactgtgct aaattttctg gccgcatacg cgcggccaga
    16621 ggttgaaaag tattatccga aaaatcgagc gacagattgc tcactcaggt gcctctactt
    16681 acgttgatca tctaccgtga cgccttgcgg cggggtgaag gtaaatttcg ctgcatccac
    16741 agccccattt tgctgggatt tcagttgata actgctgcgc tgatcgtcct gctccaccgc
    16801 gctaaactga tggattgtgc catcacgtcc cacgttaatg gtgaactgct tcagattgcc
    16861 attgctggct ttcggcgtca ggacaaagtc atcgccattc tgtttgatat tgtactgctg
    16921 ccagtcgctg gactggttgc gggcaatcag cataaacggc gtattaccgg tggcatcttt
    16981 cagccaggtt gccgtagctt gctcaacgaa cgggttatag aaccacagtg ttttaccgtc
    17041 agaaaccaga atgctttcat caggttgtgt catatgccag ttgaataagt ttggacgttt
    17101 cacccacaga tcgccctgac cttcctgcac cgccgcgccg ctaccgtcag tcactttttg
    17161 tgtgaagctg gcgtggaagc tgctgacttt atccaggcgg cttttcagat cgcttgcggc
    17221 atcagcccaa acgctgcttg ctactaagct tgagagtaat gcacaggtga tggcaatttt
    17281 tttcatcatt attcctcaaa ttacgtcact cccgatcggg agttccctct actctctatt
    17341 ccaggctaat aatcagcttg aggaaagaag aaaatactga attttcagtt gatagagtgc
    17401 cggatgcgac gctaccgcgc cttatccggc aatgcattaa ttagtcaaac ggcggtgggg
    17461 ccagcacttc acgattaccg ttgtgcccct gttcgctgac aatcccctgc gcttccatct
    17521 gttcgataat acgcgctgca cggttataac caatgcggaa ctgacgctgt acgccagaaa
    17581 ttgacgcttt gcgtttttca gtgacaaact gcaccgcctg atcgaacaac ggatccagtt
    17641 cttcagcgcc atcgaaaccg cccgcgccac cttcgctttc actgtcggaa gtgatgccat
    17701 caacatactg tgggcgacca cgcgctttcc agtcctgcac cacggcatga acttcctgat
    17761 cgcgaacaaa agcaccatgg acacgtaccg gcaacgtgga gttcggccca gagtagagca
    17821 tatcccccat ccccagcagt gattccgcgc cagcctgatc aagaatggta cgtgagtcaa
    17881 tcttactgga tacggtaaag gcgatacggg tcggaatatt cgctttaatc agaccagtaa
    17941 taacatcaac cgatggacgc tgagttgcca gtacgaggtg gatacccgcg gcacgggctt
    18001 tttgcgccag acgtgctatc agctcttcca cttttttacc taccgtcatc atcaggtcgg
    18061 caaattcgtc aaccaacacc acaatgtatg gttctttttt cagcaccgga tgctgggcat
    18121 ccatactgtc acccggcttc cagtacgggt ctggaatcgg acgcatcatg cgatcggctt
    18181 cagcaatttt ttcgttataa cccgccagat tacgcacacc cagcgcagac atcagtttat
    18241 accgacgctc catctcgtta acacaccagc gcagcgcgtt ggcggcatct ttcatatcag
    18301 taacgacttc cgttaacaga tgcggaatgc cttcataaac cgaaagctcc agcattttcg
    18361 ggtcgatcat gatgaaacgc acatcttctg gctgtgcttt ataaagcatg ctcaggatca
    18421 tcgcgttcac accgacagat ttaccggaac cggtagtccc cgcaaccaac aagtgcggca
    18481 ttttcgccag atcggcaacc accggctcac cggcgatatc tttacccagc accacggtta
    18541 atggcgacgg attatcgcgg aatttggcgt tatccaaaac ttcgcgcaga taaacggttt
    18601 gtcgtttttt atttggtaac tccagaccta catagggttt gccaggaata acttcaacga
    18661 cacgcaccgc caccgtcgaa agtgaacggg caaggtcccg tgacaagtta gaaatgcgcg
    18721 ccgcttttac gccaggagcc aggttaagtt caaagcgagt gataaccggc cccggagagt
    18781 aattgacgac atcggcttta atacggaaat cagccagacg cgcttccacc aggcgagcca
    18841 tttgttcaag cgcaaaggta tctaccggct ccacttcgct cggcggcggt gtcagcaaat
    18901 ccagagaagg cagcggcgtc gtcggtttat gcaacggacg gctgtcgcca ttacgcatca
    18961 acagcggatg aagcagggta tcctgctgct gcggtgcaac cggttgttgc ggctgttgat
    19021 actgcggctg cggagcaact ggttgttgcg gctgctgata ttgcggctgt ggcgcaaccg
    19081 gttgttgcgg ctgctgatat tgtggctgcg gcgcaacctg ctgttgtggc tgctgatact
    19141 gctgctgcgg cgcaaccggc tgttgcggct gctgatattg ctgctgtggc ggaactggtt
    19201 gttgcggctg ctgatactgc tgctgcggtg caaccggttg ttgcggctgc tgtacaggtt
    19261 caacaattgg cgtaaacaac ggttcgtgtg gaccatcatc cagcaacgct ttcattggcg
    19321 aaaattcaaa atcatccagc gagaacggat tcgccccagc cggttgttcg ccggaataac
    19381 gttgttgttg agtttgcgca aactgacgag ccagttcagc ctctgccgca gcatctgcat
    19441 cttctgcgtt tacgggcaca tcatgttgat actgttcgcc atagcgttgc tgctgtgtct
    19501 gggcgaactg acgtgccagt tcatcctgct gcatcgcatc gatttcatca tcgttatact
    19561 gatcgccaga atcatactga ttgcgctggg cttcacgggc tttctcttcc gccgcacgct
    19621 gtgagggcag cttaataccg taagacgcca gttcacgacg agttggcacg cggatacgtt
    19681 tcggtcgtgg caactgcggg ccaatcccct ctttgacttg cggacgcggt ccaccgctat
    19741 ttgccagact gaagactggc gcggcaacgg ttgcggcagc ccccgtcgcc agtgtcgctt
    19801 ttttcacgcc agatgccagc ggagaaacag cggcagcggc ttctactgga ggtactgctg
    19861 caacagaagg cgctttcaac gaagatttga tcggttctgg ttctttaacc ggttctggaa
    19921 tcggttgata ccaggccgca agttgttcac gttcacgggc tcgcttctct tccacttctt
    19981 caaaatagta aagcggcgga cgcgcgggtt ttgtctcttc tacaacgggt tcaggctcca
    20041 caacaggctg ctgttcaacg gattgcggct gttggtacaa cggctcctga gcggctggtt
    20101 gctgataagt ttgctcagtc tggtatgtag actgtggagc aaaagtggat tgctgctctt
    20161 cggcttgcca ggcgttacct gccaccggtt gttctggcgc aggggcgtaa tacggctgtt
    20221 gtgcaggctg ttccggcgca gttgcgtaat acggttgctg aacaggttgt tccgccgcag
    20281 gagcgtagta aggttgttgc gcaggttgtt cagctgcagg tgcataatac gactgctgcg
    20341 gctgtactgg ttgttgcagc ggctcattat attgcactgc aggctgcgca tattgtgact
    20401 gctgtgggta accttccggt gcaggagcaa taaccggctc tcccgtttgt ggacccggta
    20461 caggctgcca ggctactgta ggttgcgagg gtggaacatc aacagaggca acaggcggcg
    20521 tctgagtcac aggttcaacc ggcgcagccc agctttgtgt cgccgtggta gaagcagctg
    20581 ctacagcgac aggttcggta attggcgcac cgtttaataa tggatcgtat tcgtcatatt
    20641 ctggctgcgt tgcacgattg cccgaaaata ggacgtcgtc cgggtcggca gccacaccgc
    20701 gtgcagtgta ggtaatctct tcgtcatcat ccatccgctt accggagaac aacgcagcgt
    20761 ctgtttgccg ccccatcgga ttaatgaatt tttccgccaa ccgtttacga cgcgctagcg
    20821 cgccgcgaag aatacgggca cggcgtgatt catgctgttt gccgtggttt tcatcttcat
    20881 actcttcgtc atcttcatat tcatcttcat cgacccaggt atcatcgcga cgggtacgat
    20941 tactggcgaa ggtgagaatg tttaaaatcc agccgccgag tttttcagca atggtcaccc
    21001 atgaccaacc ggtgaacagc gtcaggcccg cagcccaaac gcagagcagc gcaatagttc
    21061 ccccgctact gtgtagcagt ggttgtagcg tagtacttag taagctgcca atgacgccac
    21121 cggaggcaaa ataccagata tcgtcagcgt tgattgccgc cagaccacag gaggtaagga
    21181 tgagcgccaa aacgccaatg atgcgtagcg aaacggcaaa ataatcaatg tattcgtcgc
    21241 tggactgatg acgccaggca aaccaacaac cgccgacaat aatgacggga atggtgtaag
    21301 ccatcacgcc aaaaataaag aacagcgtat ctgccaacca cgcaccgggc atcccaccta
    21361 aattatggat aggttcatgc caggccgttt gcgaccagct ggggtccgaa gggttaaagc
    21421 ttagtaaggc agccatcaac cagacggcaa acaggacaat aaggatcagc aacgcctcca
    21481 gaaggcggcg gccgctactt aactttgtca atgtgacttc tttgtcttca gtgtattcct
    21541 ggctcaagaa aggctctcca ggttacaggc ttttcctgcc cgatgctaaa acggacaaca
    21601 gtaccaggtt accccagtac tgttgctgta tggattaaca ggagtgtaat caaaatacgc
    21661 cgattttgca cctgttccgt gttagcgcgt cttaataacc agacgattac tctgcttgac
    21721 ttcttccata acaacgtatg tccgtgtgtc attgacgcca ggcagacgca gcagggtttc
    21781 ccccagcaac ttacggtaag ctgacatatc cggcacgcgt gttttcaaca ggtagtcgaa
    21841 atcaccagat actaaatgac actcctgaat ttcttcaagt ttttgtacag cggtattgaa
    21901 ttgttcaaac acatccggtg cgccacgatt cagagtaatc tcaacgaata ccagaagtga
    21961 tgcatccaga taatgggggt tcagcagcgc cgtatagccc tgaataaacc cttgtctttc
    22021 cagccgacgc acacgctcaa ggcacggcgt tggggaaagt cccacacgtt tagaaagctc
    22081 gacgttagaa atacgcccat ccttttgcaa ctcattaaga atgttacgat cgatacggtc
    22141 gagatctttg ccagggcgct tcttgctatc taccattatt attgtctctc tgtattcctt
    22201 ccctactcct gtctgaccct gaaacatcac tgttcagagt cgctacccgt cacgatagac
    22261 cgaaatcatg aagattcacg gcgcgcaatg cctgggtcta cggtgagaag accgttgcct
    22321 tgtggcagct atcgacatca cgaatggcgt ctgtccacgc catacgtaga tttcactagc
    22381 acggtaaaca tggtatttac atgcatggtt atgcgcatat aagaatactg tttttttctt
    22441 cctcgaatgt tttcgcaaaa caccagggga ttgtcaaagc aaaacatcga tttttagtac
    22501 aacatgccag atattcatta acaggggcgt ttattcatca tttaatcggc ttataaagcg
    22561 ggaaatttaa gtagcagagg tgataatgac gggagaattt gacctgctta tcgatgctgc
    22621 cgattgcaca aattgttaac aaaatcgtca tactcttttt ttacgtctgt aaattcccta
    22681 caatcctgcc cattgtctgc caacaactat ggggatctca tgggcacgac caaacacagt
    22741 aaactgctta tcctgggttc aggcccggcg ggatacaccg ctgctgtcta cgcggcgcgc
    22801 gccaacctgc aacctgtgct gattaccggc atggaaaaag gcggccaact gaccaccacc
    22861 acggaagtgg aaaactggcc tggcgatcca aacgatctga ccggtccgtt attaatggag
    22921 cgcatgcacg aacatgccac caagtttgaa actgagatca tttttgatca tatcaacaag
    22981 gtggatctgc aaaaccgtcc gttccgtctg aatggcgata acggcgaata cacttgcgac
    23041 gcgctgatta ttgccaccgg agcttctgca cgctatctcg gcctgccctc tgaagaagcc
    23101 tttaaaggcc gtggggtttc tgcttgtgca acctgcgacg gtttcttcta tcgcaaccag
    23161 aaagttgcgg tcatcggcgg cggcaatacc gcggttgaag aggcgctgta tctgtctaac
    23221 atcgcttcgg aagtgcatct tattcaccgc cgtgacggtt tccgcgcgga aaaaatcctt
    23281 attaagcgtc tgatggataa agtggagaac ggcaacatca ttctgcacac caaccgtacg
    23341 ctggaagaag tgaccggcga tcaaatgggt gtcactggcg ttcgtctgcg cgatacgcaa
    23401 aacagcgata acatcgagtc actcgacgtt gctggtctgt ttgttgctat cggtcacagc
    23461 ccgaatactg ccattttcga agggcagctg gaactggaaa acggctacat caaagtgcag
    23521 tcgggtattc atggtaatgc cacccagacc agcattcctg gcgtctttgc cgcgggtgac
    23581 gtgatggatc atatttatcg tcaggccatt acttcggccg gtacaggctg catggcagca
    23641 cttgatgcgg aacgctacct cgatggttta gctgacgcaa aataatttta caaatcagta
    23701 acaaaagtaa agaaggcgac accatgcgac tatgggtcgc ctttattttt tccccgttgt
    23761 aacattgctc tgcaaataat tctgataact cacctgctaa gcgtgcaatg aataaatccc
    23821 gtcaaaaaga gttaacccgc tggttaaaac agcaaagcgt catctcccaa cgttggctga
    23881 atatttctcg tctgctgggc tttgtaagcg gcatattgat cattgcccag gcctggttca
    23941 tggcgcgaat tctgcaacat atgattatgg agaatattcc ccgtgaagcc ctgctgcttc
    24001 cctttacgtt actggttctg acctttgtac tgcgcgcatg ggtggtctgg ttacgcgaac
    24061 gggtgggtta tcacgccggg cagcatatcc gctttgccat ccgccgtcag gttctcgacc
    24121 gtctgcaaca agcagggcca gcgtggattc agggtaaacc tgcggggagc tgggcgacgt
    24181 tggtactcga gcaaattgac gatatgcatg attactatgc acgctacctg ccgcaaatgg
    24241 cgctggcagt gtcggtgccg ttgctgattg tggtggcgat cttcccctct aactgggctg
    24301 cggcgctcat tctgctgggc acagcaccgt taattccgtt gtttatggcg ctggttggga
    24361 tgggggctgc cgatgctaac cggcgtaact ttctcgctct tgctcgctta agtgggcatt
    24421 tcctcgatcg cctgcgcggc atggaaacat tgcgtatttt tggtcgtggt gaagctgaaa
    24481 ttgaaagtat tcgttctgct tcggaagatt tccgccaacg gacaatggaa gtgctacggc
    24541 tggcgttttt atcatccggc attctcgaat tttttacctc gctgtcaatt gctctggtgg
    24601 cggtctactt tggtttttcc tatctcggcg agctggattt tggtcactac gataccggtg
    24661 tgacgctggc tgcgggtttt ctggccctga tccttgcgcc agagtttttc cagccattgc
    24721 gcgatctcgg tacgttttat catgctaaag cccaggctgt tggtgcagct gacagtctga
    24781 aaacgtttat ggaaaccccg ctcgcccatc cgcagcgtgg cgaggtggaa ttagcatcga
    24841 ccgatccggt gaccattgag gccgaggatc tgtttatcac gtcgccggaa ggtaaaacgc
    24901 tggccggacc gctgaacttt actttgccag caggccaacg tgcagtgttg gttggtcgca
    24961 gcggttcagg taaaagctca ctgctgaacg cgctttctgg ttttctctca tatcagggat
    25021 cattacgaat caacgggata gaattacgcg atttatcacc agaatcatgg cgtaaacatc
    25081 tctcctgggt tgggcaaaac ccacaattac cggcagcaac attgcgggat aacgtactac
    25141 tggcgcgacc tgatgccagc gaacaagaat tacaagcagc gctggataac gcctgggtca
    25201 gcgagtttct accgctcctg ccacaaggcg ttgatacacc tgttggcgac caggctgccc
    25261 gcctttccgt ggggcaggcg cagcgcgtgg cggtggcccg tgcgttacta aatccctgtt
    25321 cgctattact gttggatgaa cccgctgcca gccttgatgc tcacagtgaa cagcgcgtaa
    25381 tggaggcgct gaatgccgcc tctctgcgcc agacaacgtt aatggtcacc caccagttag
    25441 aagatcttgc tgactgggat gtcatttggg ttatgcagga tggccggatt attgagcaag
    25501 gacgttacgc ggaattaagt gtggctggtg gcccattcgc cacattactg gcccatcgtc
    25561 aggaggagat ttaaatgcgc gctttgctac cctatctggc actgtataaa cgtcataaat
    25621 ggatgttaag tcttggtatc gtgctggcaa ttgtcacgct gctcgccagt atcggtctgt
    25681 tgacactttc cggctggttc ctctcggcct cagcggttgc gggggttgcc ggactgtaca
    25741 gcttcaacta tatgctaccc gctgcgggcg tgcgtggcgc agcaatcacc cgtactgccg
    25801 ggcgttattt tgaacgtctg gtaagtcacg acgcgacttt ccgcgtgttg cagcatctgc
    25861 gcatttacac cttcagcaaa ttgctgcccc tctcccctgc cggactggcg cgctatcgtc
    25921 agggcgaatt gctcaatcgc gtggtggcgg atgttgatac gctcgatcat ctttatctgc
    25981 gcgttatctc gccgctggtg ggcgcttttg tggtgattat ggtggtgaca atcgggttaa
    26041 gtttccttga tttcaccctc gcctttacgc tgggcggcat tatgttactg acgcttttcc
    26101 tgatgccacc gctgttttat cgtgcgggaa aaagcaccgg gcaaaatctg actcatcttc
    26161 gcggacagta tcgccaacaa ctgacggcct ggctgcaagg gcaagctgag ctgaccattt
    26221 ttggcgccag cgatcgttat cgcacgcaac tagagaatac agaaattcaa tggctggaag
    26281 cgcaacgccg tcaatctgaa ctgaccgcat tgtcgcaagc gataatgctg ctcattggcg
    26341 cgttagcggt gatcctgatg ctgtggatgg cgtctggcgg cgttggcggc aatgctcaac
    26401 ccggcgcgtt aattgccctg tttgtcttct gcgcgttagc cgcgtttgaa gcactggcac
    26461 cagtaacggg tgcatttcag catctggggc aagtcattgc ctctgccgtt cggatcactg
    26521 atttaacgga tcaaaaaccg gaagtcacct tccctgatac ccaaactcgt gttgccgatc
    26581 gcgtttcgct gacattacgg gatgttcagt tcacttatcc ggagcaatcc caacaggcac
    26641 ttaaagggat ttctcttcag gtaaacgccg gggaacatat tgcgattctc gggcgaaccg
    26701 gatgcggcaa atcaacactg ttacaactgc tgacccgcgc ttgggacccg caacagggag
    26761 agattttgct taacgatagc cccatagcca gcctgaatga agcggctcta cgacagacta
    26821 tcagcgttgt tcctcagcga gtgcatctgt ttagcgccac gctgcgtgat aatctgttac
    26881 tcgcctcgcc tggcagtagt gatgaggctc tggcggagag cttgcgtcgc gttggtctgg
    26941 agaagctgct cgaggatgca ggtctcaaca gttggttagg tgaaggcgga cgccagctct
    27001 ccggtggtga actgcgccgt ctggctatcg cccgtgcgct gttacatgat gcgccactgg
    27061 tgttgctgga tgaacctacc gaaggcttag atgccacaac cgaaagccag atccttgaat
    27121 tgcttgcaga aatgatgcgt gagaaaacgg tgttaatggt cacccatcga cttcgcggac
    27181 tctctcgttt ccaacaaata atagtgatgg acaacgggca aattattgag caaggtactc
    27241 acgcagagct gcttgccaga caggggcgtt attaccagtt caagcagggt ttgtaagcta
    27301 ttattgaacg atccgacttg cgtggagttt tgcggtcatg cgcctggttc agctttctcg
    27361 ccattcaata gccttccctt ccccggaagg cgcattacgt gagcctaacg gcctgctggc
    27421 acttgggggc gatcttagcc ctgcgcgcct gttaatggct taccagcgtg gtatttttcc
    27481 gtggttttct ccaggcgacc ccatcctctg gtggtcgccc gatccccgcg cggtgctatg
    27541 gccagaatca ctgcatatca gccgtagtat gaagcgattt cataaacgct cgccctatcg
    27601 tgtcacgatg aattacgctt ttggtcaggt cattgaaggc tgtgccagcg atcgcgaaga
    27661 aggaacctgg atcacgcgtg gcgtggtcga agcctaccat cgccttcacg aactcggcca
    27721 tgcccactcc attgaagtct ggcgtgaaga tgagcttgtc ggcggtatgt acggcgtggc
    27781 ccagggaacg ctattttgtg gcgagtccat gttcagccgg atggaaaatg cgtctaaaac
    27841 ggcgcttctg gtattctgtg aggaatttat cggtcatggc ggtaagctta tcgactgcca
    27901 ggtccttaac gatcacacag catcgcttgg tgcctgcgaa attccccgcc gggattacct
    27961 taattatctc aatcaaatgc gcctcggacg attgccgaat aatttctggg taccacgatg
    28021 cttgttttca ccacaagaat gaatgttttc ggcacatttc tccccagagt gttataattg
    28081 cggtcgcaga gttggttacg ctcattaccc cgctgccgat aaggattttt tcgcgtcagg
    28141 taacgcccat cgtttatctc accgctccct tatacattgc gcttttggtg cggcttagcc
    28201 gtgtgttttc ggagtaatgt gccgaacctg tttgttgcga tttagcgcgc aaatctttac
    28261 ttatttacag aacttcggca ttatcttgcc ggttcaaatt acggtagtga taccccagag
    28321 gattagatgg ccaaagaaga caatattgaa atgcaaggta ccgttcttga aacgttgcct
    28381 aataccatgt tccgcgtaga gttagaaaac ggtcacgtgg ttactgcaca catctccggt
    28441 aaaatgcgca aaaactacat ccgcatcctg acgggcgaca aagtgactgt tgaactgacc
    28501 ccgtacgacc tgagcaaagg ccgcattgtc ttccgtagtc gctgattgtt ttaccgcctg
    28561 atgggcgaag agaaagaacg agtaaaaggt cggtttaacc ggccttttta ttttgtgata
    28621 tgtatgaagt actttggaag tataagtccg taacttgtct cgatgtagat atactgcaca
    28681 acttgtttac agtatgagca aacaataatt atcattaaaa ttcttcttga ccgttttact
    28741 ccaactccct atagtagcgc cccgttgcca cccatgaggt ttggtagcaa aacaatttgg
    28801 tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg
    28861 gggttcgaat ccccccctca ccgccatatt taaagaagag ctcgtacgaa agtgcgagct
    28921 tttttttcgc attttatctg tctgtacggc agtgaacgcg ttcgcaaagg ttttaccgca
    28981 ccccgtcgat ttctaagctg cctgtacggc agtgaactca aatactacac gtttagcccg
    29041 ttgttaataa agcaaaacca tcccattctc ctgcagcacc cttttttatc cttaccttcc
    29101 cgcagcacta attatcaaca acttaccatc atcattaaaa aaagggtctg aacctcctcc
    29161 accccataaa caaaaagtcc gtaacctctt tcgagattac ggacttgacc aacctaccta
    29221 acaatcagat taatgcgctg cttccgcctt gtgcttttgt gcactctgga atccgtaagt
    29281 cagctcattt ttctctttat ccagcgcgac ggtgacctga ccgccgtcca ccagcgaacc
    29341 aaacagcagt tcgttggcga gcggtttttt caggttgtcc tggatgacac gcgccatcgg
    29401 acgtgcgccc attgcccggt cgtaaccttt ctcggccagc cagttacgcg cttcctggct
    29461 cacttccaga gaaacacctt tctgatccag ctgaacctgc aactcgacga tgaatttatc
    29521 caccacctga tggatcacgt cggttgacag atgatcaaac cagataatgt tgtcgagacg
    29581 gttacggaat tccggtgtaa agatcttctt gatctcctcc atcgcatcgg tgctgttatc
    29641 ctggtggata agaccaatgg atttacgctc agtttcacgt accccggcgt tggtggtcat
    29701 caccagcacc acgttacgga agtccgcttt gcgtccgttg ttatcggtca gcgtaccgtt
    29761 gtccatcacc tgcaacagaa tattgaacac gtccggatgc gctttctcga tttcgtccag
    29821 caacagcacc gcatgtggat gcttgatgac cgcatcagtc agcaaacctc cctgatcaaa
    29881 accaacgtat cccggaggcg caccaatcag acggctgacg gtatggcgtt ccatatactc
    29941 ggacatatca aagcgcagca gctcaatgcc caacgctttc gaaagctgta ccgtcacctc
    30001 tgttttcccg accccggtag ggccggcaaa caggaacgaa ccaaccggtt tatgttcgtg
    30061 acctaaacct gcacgcgcca tcttaatggc ttcagtcagc gcctcaatgg ctttatcctg
    30121 accgaagacc agcattttca ggcgatcgcc gaggtttttc agggtatcgc ggtcactctg
    30181 agaaacactc ttctctggaa tgcgcgcaat acgggccacc acggactcaa tatccgccac
    30241 attaacggtt ttcttgcgtt tgcttaccgg catcaggcgt gcgcgagcgc ccgcttcgtc
    30301 gataacgtca atggctttat ccggcagatg acgatcgtta atgtatttca ccgccagctc
    30361 taccgctgca cgcaccgctt ttgcggtata acgcacgtcg tggtgcgctt catacttcgg
    30421 tttcaggcca ttgatgattt gaacagtttc ttcgatcgac ggttcagtaa tatcaatttt
    30481 ctggaagcga cgcgccagag cacggtcttt ctcgaaaatg ttgctgaact cctgataggt
    30541 tgtcgaacca attacgcgaa ttttaccgct ggagagcaac ggtttgatca ggttagccgc
    30601 atcgacctgg ccaccagacg ctgcacccgc accgataatg gtgtggatct catcaataaa
    30661 caggatgctg ttagtgtcct gctccagctg cttgagcaac gctttaaaac gtttttcaaa
    30721 gtcgccgcga tatttagtgc ccgctaacag agaaccgata tcgagagagt aaatcgtaca
    30781 gtcagccatc acttccggca catcgccctg aacaattcgc caggcaagac cttccgcaat
    30841 cgcggtttta ccgacaccag attcccccac cagcagcggg ttgtttttac ggcgacggca
    30901 gagaacctga atagcacgct ccagctcctt ctcacgacca atcagtgggt cgattccgcc
    30961 cacgcgcgca agctgattca ggttcgtcgt gaaattctcc atacgttcct ccccaccagc
    31021 ttgttcttcg ctgtttggct ggctgccagg atcagaagac tgtgtcggct cgtctttacg
    31081 cgtgccatga gagataaagt tcaccacatc gagacggctg acttcatgtt tacgcaacag
    31141 atatgccgcc tgcgactcct gttcgctaaa gatagcgacc agaacgtttg caccggttac
    31201 ctcattgcga ccggaggact ggacatggaa gaccgcacgt tgcagtacac gctgaaaact
    31261 cagcgtcggc tgtgtgtcgc gctcctcttc actggcaggc agaacgggtg tggtttgttc
    31321 aataaaggct tccagttcct gacggagcgc aaccaaatcc acagaacacg cttccagcgc
    31381 ctcccgggca gatgggttac tgagcagcgc cagtaacaag tgctcgacgg tcataaactc
    31441 atgacggtgc tcgcgcgctc tggcgaaagc catatttaaa ctgagttcca gttcttgatt
    31501 gagcataggc acctccccca atttttatgc ctgcattcag gctttttcta gcgtacacag
    31561 caatggatgc tcattctccc tcgcgtactt gttcaccatc gccactttgg tttctgcaac
    31621 ctcggcggta aagactccgc aaatggcctt cccctggtag tgaacagcga gcatcaattg
    31681 cgttgcacgt tctacatcat aagaaaagaa tttttgtaac acgtcaataa caaactccat
    31741 cggagtgtaa tcatcattga ctaatatcac tttatacata gatggcggtt ttagcgcgtc
    31801 gcgaactttt tcttccgcca gttgatcaaa gtccagccag tcgttcgttt tacccattgt
    31861 cagttatcat cttcggttac ggttatcggc agaacgccct gccgctgaca agagcttatt
    31921 tgcctgacca atctactcca catgatagat aactatcatc tatcgttggc atcagcgaca
    31981 tctgtcacat tcctgtcaat agcgttaact gcttcaaatt tttgattcat acctgccccc
    32041 tggcccctgt caaacgcttg acggctcgcc ctaattctct aaattgtatt tctagagttg
    32101 gcgaggtttt gaacagcccc ctctctgacc ccggtttatt ccatcttact tgtataagat
    32161 ttgcgaagga tgtcgaagca tggaaaaggg tactgttaag tggttcaaca atgccaaagg
    32221 atttggtttc atctgccctg aaggcggcgg cgaagatatt ttcgctcatt attccaccat
    32281 tcagatggat ggttacagaa cgctaaaagc tggacaatcc gttcagtttg atgtccacca
    32341 ggggccaaaa ggcaatcacg ccagtgttat tgtgcccgtc gaagtagaag cggcagtcgc
    32401 atagctcttc tgtctcattg tgtacatcct aaaggcaaaa tgccagcccg atcggctggc
    32461 atttttatct caaaaattac tctcgtgcca gagcatctac tggatccagt cgtgccgcat
    32521 ttcgtgcggg taaccatcca aataaaatcc cggtgaccgt cgagcagaga aacgccagca
    32581 gcagtgccaa cggtgaaaaa ccaatctccc agccgggtaa gaaaagctgc aaggtgaaag
    32641 caattaacag tgacagtgtt attcccaacg cgccaccgac caggcaaacc agtacggctt
    32701 cgatcaggaa ctgttgcaaa acatcgcttg ctcgcgcacc tacagccatg cgaatgccaa
    32761 tttcccgcgt ccgctcggtc actgacacca gcataatatt cattacacca ataccgccca
    32821 ccaccagcga aatcaccgcc accagcgtca gaaacagttg taaagtacgt gtggtctttt
    32881 caacagtttt caagacgccg tccatgttcc aggtaaagaa atccttcttt ccgtggcgca
    32941 gtgaaagtaa acgcgtgagt tgctgttccg cctcggcgct gtcaaatcct tcttttaccc
    33001 tgacagtaat ggagttcagc cacgactggc ccataactcg cccggacatc gtgctgtaag
    33061 gtagccagac acgcagcact ttactgctac caaacatcga ctgtttttct tccgccacac
    33121 caatgactct ggcgggcata ttgccgacca gaatcacctc gccaaccaca tctgctttat
    33181 gggggaaaag ctggcggcga gtattactgt cgagaaccac gacctgcgca cgaccgttca
    33241 gctgctcctg attaaaggtg tttccttcac tgaaggtcat gccatagaca ttaaaataat
    33301 cgccgctcac gccattggca ctggcagcaa catcaacatt gttataacgc aggcgcaggt
    33361 tttgcgagac ggcaggtgtg gctgaggcga cccacggttg tttttggatg gcgattaagt
    33421 cgtcgtactt cagcgcctgc tgatattgcg gatcgtcatc gccaaaatct ttcccgggat
    33481 agacatcaat agtattcgta ccaatagaac gaatatccgc cagcaccatt tgtttggcgg
    33541 cgtcacccac cacgacaatg gaaaccaccg acgcaatacc gataataatc cccagcatgg
    33601 tcagtaaagt acgcatttta ttcgctgcca gcgcccgcca tgccatcgtc agcgcctcgt
    33661 taaaaccgct gacaaactgc cgccagccag acaccgtgtt gacaacaggc tccgtcccgc
    33721 cagcaacatt cactttttca atggcgggag gattgcgcac aatttcgccg tcgcgaattt
    33781 cgatcacccg ctcggcctga gcagcgacct gcggatcgtg ggtgacgata atcaccgtat
    33841 gcccacgatc gcgcagctga tgcaggatcg ccatcacctc ttcgccagaa tggctgtcca
    33901 gtgcgccggt tggttcatcg gcaagaatta cctgaccacc gttcatcaat gcccgcgcga
    33961 tgctgacgcg ctgttgctga ccacccgaaa gctgtgccgg ataatactct gtacggtctt
    34021 ccagccccag ccgttgcagc aactcctggg cacgaagcag tcgctgtttc cgctcaagac
    34081 cagcatagac ggcgggtact tcaacgttct gctcggcggt taaatgcgaa agtaaatggt
    34141 aacgctggaa aataaagccg aaatgctcgc ggcgcagttg cgccagcgca tcggcgtcca
    34201 gtgtggcaac atcctgaccg gcgacgcgat aggtgccgct ggtggcctta tccagacagc
    34261 cgagaatatt catcagggtc gatttaccgg aacctgaagc gccaacaatc gctaccatct
    34321 caccggcata aatatcgagg gtgatgccct tcagcacctc aacctgctca tcaccggcag
    34381 gatagctgcg acgaatatcc tttaattcga gcaaaggcgt cattgtgcag ctcctggttt
    34441 ggcctcacca atcaccactt catcgcccgc ttcaagccct ttgacaatct caacatcggt
    34501 atcgttacgt gcgccaatcg tcacttcacg ctcgcgtgtt tcaccattac gcaacaattt
    34561 gactttataa cgattatcgc caaccggatc gcctaacgcc gacagaggga tcgtcagcac
    34621 atttttcaca tcggtgagct gaatatgcac ttgcgcagtc atatccagcc gcagcaaacc
    34681 attggggttg gggacttcaa aacgggcgta atagaaaata gcgtcgttaa ccttttccgg
    34741 cgtcggtagt acatccttga tttgcccctc gtagcgcgtc agtggatcgc caagtaccgt
    34801 aaaccaggct ttttgccccg gcttcaggtg gattacatcc gcttcagaaa cctgcgcttt
    34861 taccagcatg gtgctcatat ctgccagcgt cagaatgttc ggtgcttgtt gtgcggcaat
    34921 caccgtctgg ccttgcagag tggtgatttg cgtgacttcc ccggccatcg gggcaacgat
    34981 gcgagtgtaa tcgagattgg ttttagccgt atcgagagag gcctgattgc gcttgatttg
    35041 cgcgtcaatg gtgccaattt gcgcctgttt cacagccatc tccgtcgcgg cggtgtcgag
    35101 atcctgctgt gaaaccgcct gcgtttgtgc cagacgttgc tgacgggaat acgtcacccg
    35161 cgccagtttc agctccgctt ccgcctgctg ccgctgcgca cgtagctcca tcagcgttgc
    35221 ttcgacctcc ttaatctggt tttcagcctg ttcaggatca ataaccccta aaagctggtc
    35281 tttttttact ttatcgccaa tcgccaccga cagagttttc aactgaccgc tgacctgcgc
    35341 gcccacgtca accttacgca gcgcgtccag ctttccggtc gccagcacgc tttgctgtaa
    35401 atcaccgggg cgcacaatca gtgtctgata agtcggcacg ggtgcgttaa gaattctcca
    35461 taacgtaatc agtccggcga tgactatcac cagcgcaata acgtaacgct tcttcacggt
    35521 tttccgcttt ttcataaata ttctgaatac tccatatgcc aatccgggtc attaacttca
    35581 acgaaatatc aacaaaaccc taaaatttac gttagtaatc acactattga tgtttggtta
    35641 agatacgcaa tggtgaaatc gacgtcatgt ataaccattg atttcatgaa tatgtcgcag
    35701 ctaactgaac ggacctttac gccatctgaa tctctcagca gcctgtcact ttttcttagt
    35761 ctggcacgtg gacagtgtcg gccgggtaaa ttctggcatc gccgtagttt tcgccagaaa
    35821 tttttgctgc gctcgttgat tatgccgcgt ttaagcgttg agtggatgaa cgaactttcc
    35881 cactggccta atctcaatgt gttgttaacg cgccaaccgc gactgcctgt gcgtctgcat
    35941 cgcccttacc ttgcggcgaa tcttagccgt aagcaattgc tggaggcgtt acgttaccat
    36001 tatgcgttac tccgcggatg tatgtcggcg gaagaattca gcttatattt gaataccccc
    36061 gggctgcaac tggctaaact ggaaggcaaa aacggcgagc agttcacgct tgagctgacc
    36121 atgatgatct caatggataa agaaggtgac agcacaatcc tgttccgcaa cagcgaaggt
    36181 attcctctgg cagagctcac ttttaccctg tgtgaatatc aggggaaaag aacgatgttt
    36241 attggcggac tgcaaggcgc aaaatgggaa atcccacatc aggaaatcca gaatgcgacg
    36301 aaagcctgcc acgggctatt tcccaaacgc ctcgtgatgg aagcggcctg tctgtttgcc
    36361 caacgtttgc aggtagagca gattattgcc gtcagcaatg aaacgcatat ttaccgcagc
    36421 ctgcgttatc gtgataaaga aggcaagatc catgccgatt acaacgcttt ctgggaatcg
    36481 gttggcggcg tatgtgatgc tgaacgccat taccgccttc cagcacagat agcacgaaaa
    36541 gagattgccg aaatcgccag taaaaaacgg gctgaatacc gtcggcgcta tgagatgctc
    36601 gacgctattc agccacaaat ggccacgatg tttcgcggtt aatccgcgcg accgcgcgcc
    36661 agccacagca cgcgtgagaa catttttttc agcagcgtcg gtacggagtc cacaccacga
    36721 cgtccggcct ccattgccac ttcaatggca agatcgggtt tggaagagcg atggatcgct
    36781 ttcgagataa ttttgcgcag attcatcggt acattttccg ggatttgcgc cacgcggtgg
    36841 aacacatcgg aaaatccctg gcgatacata aaatgttcca tatccagcgc cggtaacgcc
    36901 gttaaatgtt ctcgttcggc ttcccgatcg ttattcaaca ggctgcgtac cgtagcggca
    36961 tatttcttcc ctgcttcatc gccatcgacc agtacatgcc attcaatccc cattcggcgg
    37021 gcaaatttaa ccagtggctt tagcccggac tgggcaaact caatgacctt gatcccttcg
    37081 gcatcgaaat ggtgtccaca ctggcgcgcc agttcattga taacccaggt ttccgtttcc
    37141 ccttccacca gcaaccagca gcgagcaaac agcgatgacg gacggttaaa acgaatgtga
    37201 aaggatatgc gtcggctatc ttcggtactc aagccactcg gccccagacg ccaggcggca
    37261 acgcgcgagg actcacgtac cagtcggcaa acatgctcta ccggcgttaa cgaaagcaac
    37321 tcacccgagt tggtggtggc aatgcgctgc aatggcagaa gattcaacag atgccaggca
    37381 actgaaagca taatggggtg taaacgggtt tctggatctt caattagcaa taacggacgc
    37441 gcgtctttat ccagtctcaa tgtgcctttt gcctgcaata aagtagcaaa caggccgagc
    37501 aaaataaccc gatacgagcg ccctccaggt cggtcaatca tccggttgat gatgtccaga
    37561 tagcgccagc ttcgttgctc attgctggcc cgccgccgca ttaaacgata tcgcgcctgt
    37621 ccagccccct gctcagagaa ataatgctca agcaactgca ccattgcgga aagtccctga
    37681 cgaatctgtc catcagagag attttgcgga tgtgaggaca actcacgggc gaggaaatcg
    37741 agctggcgcg cggtgacttc cacattggga acatttggca ccgtgccgtt acgaatacgg
    37801 cgcataaaac gggcatcacg caagcgcagc accggcatta aacgcaccag atggcgtgcc
    37861 tgatcgttaa tatcctcgac atcaatcgga tgtccgtctt tatcgagaaa actgcgcagt
    37921 gtcatcacgc tgccgtcttc cgcactctcc ccttccagac gataaaaaat acggtgatag
    37981 ccatcggtgc atggcgtcca gcacgcttcc agcggccgat aacggcgaac ccgatgtcgg
    38041 cctggcagcg attcgcggaa ggtcaaaata atatgcagat gatgttctcg cccgttgata
    38101 tctcccggcg ggaaccagaa atcgtcgcgc tcaaaatggt agagatctga ttctggcgat
    38161 agcagcaggg ttaaggcgtc cagcaagctg gatttacccc acgcgttctc cccaatcagg
    38221 acgttgtttt gttcgagcat caacgacaaa cggttgatac cgcgaaaacc cacaatttca
    38281 acgcgctcaa gaatcatatc ccctccagaa atatgtgact tatcctttac gctggcagta
    38341 taacgggaaa ggccttgcta atacgtgatg acatcacatt taagcatggc aaatgtctga
    38401 aaaatgagcg aataatcgcg ttatactccc tcttgctgat aagtctgcaa aaacaacaaa
    38461 atcaaataat tagcatgata aattattttt tattacgcct aaaggattaa atcttttgct
    38521 tgcactacac tgtcaatcag acaaatgact atatggggca acccgctgtt gccctaaatc
    38581 aaatttactg gatttattta tctggtgaat gactcgcctt cgtttttaaa atagccgcgc
    38641 attatttccg tcgtcacttt tttacgacga ccctataaaa cgaccatatt tttcacaggg
    38701 tcaattttta attgaggtgg atatgttcag aaaattagca gctgaatgtt ttggtacttt
    38761 ctggcttgtt tttggtggct gtggtagtgc tgtactggcc gcaggcttcc cggaattagg
    38821 cattggtttt gccggcgtgg cgttggcgtt cggtctgacc gttctgacga tggcctttgc
    38881 tgttggtcat atttctggtg gtcattttaa cccggcggtc actattggtt tatgggctgg
    38941 cggacgtttt ccggcaaaag aagtcgttgg ctacgtaatt gcccaggttg tcggcggtat
    39001 tgttgcagcg gcgctgctgt atttaattgc cagtggtaaa acgggttttg acgcggcagc
    39061 cagcggtttt gcttctaacg gttatggcga gcattcacca ggcggttatt ccatgctttc
    39121 cgcgctggta gttgaactgg tattgagtgc aggtttcctg ttggtgatcc acggcgcaac
    39181 cgacaaattc gcgccggcag gttttgcgcc gatcgctatt ggtctggcct taaccctgat
    39241 tcacttaatt agtattccgg tgactaacac ttctgttaac ccggcgcgca gcaccgcggt
    39301 tgctatcttc cagggcggct gggcattaga acaactgtgg ttcttctggg tggtgccaat
    39361 tgtcggcggc attatcggtg gtctgattta ccggaccctg ctggaaaagc gtgattaatt
    39421 aacgcagaag gcctggcgta atgtcaggcc tttttttaag atgttgttat atgttgattc
    39481 atcatcttgt tgcgctcctt tcatgagcta agccacatat tgccactggc gcaaggagcg
    39541 cgcagggggc ggccaatcgc cgccgcccca tgcacccccg ggctctggcg aacaaaatcg
    39601 ccgctgcgcg gtgccctcga cttatccctt acggctaccg ggtcgggcgc gaggtaacat
    39661 ccctgtaaaa cgcgccctca gcccacatcc atgtgggctg ccccggcctt cagggaacgc
    39721 ctcagcgatt ttgacgccac caacatcggt gcagtttgta attaaagcgc aagaatatct
    39781 ttaaatgaaa acgaatactc tactgctcct tatgcataac atttcctttt aattgcaact
    39841 ttactcgtcc ttccgctttc agtagtgtgt catcgcgcat ttcgacttca tctttgcaag
    39901 gactgggttt tcatgttttc tgggctgtta atcattctgg ttcccctgat tgtgggttac
    39961 ctcattccgc ttcgccaaca agctgcgtta aaagttatta atcagctatt aagctggatg
    40021 gtttacctta ttctcttttt tatgggtata agtctggcgt tcctcgataa cctcgccagt
    40081 aacctgttgg cgattctgca ttattctgcc gtcagtatta ccgttatttt actgtgtaat
    40141 attgccgccc tgatgtggct ggagcgaggc ctgccgtggc gcaaccacca tcagcaagaa
    40201 aaactcccgt cgcgtattgc gatggcgctg gagtcgctaa aactgtgcgg cgtagtagtg
    40261 attggttttg ccattggtct gagtggactg gctttcttac aacacgcgac cgaagccagt
    40321 gaatacacgt taattttgct acttttcctc gttggtattc agttgcgcaa taatggcatg
    40381 accttaaagc agattgtcct taatcgccgg ggaatgattg tcgccgtggt ggtggttgtc
    40441 agttcattaa ttggtggttt aattaacgcc tttattcttg atctccccat caataccgcg
    40501 ctggcaatgg cctccggttt cggctggtat tctctttccg gtattttatt gaccgaatct
    40561 tttggtccgg taatcgggag cgcggcgttt tttaatgatc tggcccgtga actgattgct
    40621 attatgttga tccctgggct gattcgccgc agccgctcta ctgcactggg cttatgcggt
    40681 gccacatcaa tggatttcac cctgcccgtt cttcaacgta ctggcgggct ggatatggtc
    40741 ccggcggcaa ttgttcacgg ttttattctt agcctgttag tgccgatcct catcgccttt
    40801 ttctctgcgt aatacctctc tggcggtaga ttcctgccgc caaaattgcg ctaaatcaat
    40861 ctcccttaaa gttgcatgaa aaatcccttt tatccccgcg ttaagcgtct taaccttaaa
    40921 catgtatatt aaatataact ttaaaaggtg tgatcatgtt ttgtgtgcaa tgtgaacaaa
    40981 ctatccgtac tccggcagga aacggctgct catacgcgca ggggatgtgt ggtaaaacgg
    41041 cggaaacttc tgaccttcag gatttactca tcgcggcgct gcaagggctt tctgcctggg
    41101 cggtaaaagc gcgtgaatac ggcatcatca accacgatgt tgacagcttc gcgccacgtg
    41161 cctttttctc aactctgacc aacgttaact tcgattctcc gcgtattgtc ggctacgctc
    41221 gtgaagcgat tgccctgcgc gaggcgctga aagcgcaatg cctggctgta gatgccaacg
    41281 cgcgcgtcga taacccgatg gctgacctgc aactggtgag cgacgatctc ggcgagctgc
    41341 aacgtcaggc agcagaattt acccctaaca aagataaagc ggcgattggc gaaaacattc
    41401 tcggcctgcg tctgctgtgc ctgtatggcc tgaaaggtgc ggcggcctat atggaacacg
    41461 cgcatgttct cggtcaatac gacaacgata tttatgccca gtatcataaa atcatggcgt
    41521 ggctggggac ctggcctgcc gatatgaacg cgcttcttga gtgttcaatg gaaatcggcc
    41581 agatgaactt caaagtgatg agcattctgg atgcaggcga aaccggtaaa tacggtcacc
    41641 cgacgccaac ccaggtcaac gtcaaagcga cggcgggtaa atgcattctg atttccggtc
    41701 acgatctcaa agatctctac aacctgctgg aacagaccga aggcacgggc gttaatgtct
    41761 acacccacgg cgaaatgctg cctgcgcatg gctacccgga gctgcgtaaa ttcaagcatc
    41821 tggtcggtaa ctacggcagt ggctggcaga atcagcaagt ggagttcgct cgtttccctg
    41881 gccccatcgt gatgacctcg aactgcatca tcgacccaac cgtaggcgct tatgacgatc
    41941 gtatctggac ccgcagcatt gttggctggc ctggcgtgcg tcatctggac ggtgatgatt
    42001 tctctgcggt tatcacccag gcgcaacaga tggcaggctt cccgtacagc gaaattccgc
    42061 accttatcac cgtgggtttt ggtcgccaga cgctgcttgg cgctgctgat acgctgattg
    42121 atctggtgag ccgtgaaaaa ctgcgtcata tcttcctgct tggtggctgt gacggcgcac
    42181 gcggcgagcg ccactacttc accgatttcg ccaccagcgt gccggatgac tgcctgatcc
    42241 tgaccctcgc ctgtggtaaa tatcgcttta acaaacttga gtttggcgat atcgaaggtc
    42301 tgccgcgtct ggtagatgca ggtcaatgta acgatgctta ctcagcgatt attctggctg
    42361 tcactctggc agagaaactg ggctgcggtg tcaacgatct gccgctgtcg ctggtgctct
    42421 cctggtttga acagaaagcg atcgtcattc tgctgacgct gctttctctg ggcgtgaaaa
    42481 atatcgtcac cggcccgact gcacctggtt tcctgacacc agacctgctg gcggtgctga
    42541 acgagaaatt tggcctgcgt tctatcacca ctgttgaaga agacatgaag caactgttga
    42601 gcgcgtaagg agtttgtcga tgacgatgcc aacgaatcaa tgcccgtggc ggatgcaggt
    42661 tcatcacatt acgcaagaaa cgccggatgt gtggacgatt tccctgattt gccacgatta
    42721 ctacccatat cgcgccgggc aatatgcact ggtcagcgtg cgaaactcag cggaaacgct
    42781 gcgtgcttac accatttcct ccacgccagg cgtgagtgaa tatatcaccc tgaccgtgcg
    42841 gcggattgat gacggtgtcg gctcccagtg gctgacgcgc gatgtaaaac gcggtgatta
    42901 tctctggctt tcggacgcga tgggggaatt tacctgcgac gataaagcag aagataaatt
    42961 cctgttgctg gcggcaggct gcggcgtcac gccgattatg tcgatgcgtc gctggcttgc
    43021 gaagaaccgt ccacaggccg atgtgcgggt gatctacaac gtgcgtacgc cgcaggatgt
    43081 tattttcgcc gatgagtggc gtaactatcc ggtaacgctg gtggcagaaa ataacgttac
    43141 cgaaggcttt atcgctggtc gtctcactcg cgaactgctg gcaggtgtac ctgacttagc
    43201 ttcacgtacc gtgatgacct gcggcccggc tccgtatatg gattgggtag agcaggaagt
    43261 gaaagcgctc ggcgtgacgc gtttctttaa agagaaattc ttcaccccag tagcagaagc
    43321 ggcgaccagc ggtctgaaat tcaccaaact gcaaccggca cgagaatttt acgccccggt
    43381 tggcaccacg ctactggagg cgctggaaag caataacgtt ccggttgtcg ctgcctgccg
    43441 tgcgggtgtt tgcggctgct gtaagacaaa agtggtttcc ggtgaatata cggtgagcag
    43501 tacaatgacg ctgaccgacg ccgaaatcgc tgaaggttac gtactggcct gctcctgcca
    43561 tccgcagggg gatttggttc tcgcataatc gccttatgcc cgatgatatt cctttcatcg
    43621 ggctatttaa ccgttagtgc ctcctttctc tcccatccct tccccctccg tcagatgaac
    43681 taaacttgtt accgttatca cattcaggag atggagaacc atgaaacaaa cggttgcagc
    43741 ttatatcgcc aaaacactcg aatcggcagg ggtgaaacgc atctggggag tcacaggcga
    43801 ctctctgaac ggtcttagtg acagtcttaa tcgcatgggc accatcgagt ggatgtccac
    43861 ccgccacgaa gaagtggcgg cctttgccgc tggcgctgaa gcacaactta gcggagaact
    43921 ggcggtctgc gccggatcgt gcggccccgg caacctgcac ttaatcaacg gcctgttcga
    43981 ttgccaccgc aatcacgttc cggtactggc gattgccgct catattccct ccagcgaaat
    44041 tggcagcggc tatttccagg aaacccaccc acaagagcta ttccgcgaat gtagtcacta
    44101 ttgcgagctg gtttccagcc cggagcagat cccacaagta ctggcgattg ccatgcgcaa
    44161 agcggtgctt aaccgtggcg tttcggttgt cgtgttacca ggcgacgtgg cgttaaaacc
    44221 tgcgccagaa ggggcaacca cgcactggta tcatacgcca caaccagtcg tgacgccgga
    44281 agaagaagag ttacgcaaac tggcgcaact gctgcgttat tccagcaata tcgccctgat
    44341 gtgtggcagc ggctgcgcgg gggcgcataa agagttagtt gagtttgccg ggaaaattaa
    44401 agcgcctatt gttcatgccc tgcgcggtaa agaacatgtc gaatacgata atccgtatga
    44461 tgttggaatg accgggttaa tcggcttctc gtcaggtttc cataccatga tgaacgccga
    44521 cacgttagtg ctactcggca cgcaatttcc ctaccgcgcc ttctacccga ccgatgccaa
    44581 aatcattcag attgatatca acccagccag catcggcgct cacagcaagg tggatatggc
    44641 actggtcggc gatatcaagt cgactctgcg tgcattgctt ccattggtgg aagaaaaagc
    44701 cgatcgcaag tttctggata aagcgctgga agattaccgc gacgcccgca aagggctgga
    44761 cgatttagct aaaccgagcg agaaagccat tcacccgcaa tatctggcgc agcaaattag
    44821 tcattttgcc gccgatgacg ctattttcac ctgtgacgtt ggtacgccaa cggtgtgggc
    44881 ggcacgttat ctaaaaatga acggcaagcg tcgcctgtta ggttcgttta accacggttc
    44941 gatggctaac gccatgccgc aggcgctggg tgcgcaggcg acagagccag aacgtcaggt
    45001 ggtcgccatg tgcggcgatg gcggttttag catgttgatg ggcgatttcc tctcagtagt
    45061 gcagatgaaa ctgccagtga aaattgtcgt ctttaacaac agcgtgctgg gctttgtggc
    45121 gatggagatg aaagctggtg gctatttgac tgacggcacc gaactacacg acacaaactt
    45181 tgcccgcatt gccgaagcgt gcggcattac gggtatccgt gtagaaaaag cgtctgaagt
    45241 tgatgaagcc ctgcaacgcg ccttctctat cgacggtccg gtgttggtgg atgtggtggt
    45301 cgccaaagaa gagttagcca ttccaccgca gatcaaactc gaacaggcca aaggttttag
    45361 cctgtatatg ttgcgcgcaa ttatcagcgg acgcggcgat gaagtgatcg aactggcgaa
    45421 aacgaactgg ctaaggtaaa aagggtggca tttcccgtca taataaggac atgccatgat
    45481 tgatttacgc agtgataccg ttacccgacc gagccgcgcc atgctcgaag cgatgatggc
    45541 cgccccggtt ggggacgacg tttacggaga cgaccctacc gttaatgctc tgcaggacta
    45601 tgcagcagag ctttccggta aagaagccgc catttttctg cctaccggca ctcaggccaa
    45661 cctggtcgct ctgctcagtc actgcgaacg tggcgaagag tatattgtcg gtcaggccgc
    45721 gcataactat ctgtttgaag ccggtggcgc agcggtgctg ggcagtattc agccgcaacc
    45781 catcgacgcg gctgccgacg gcacgctacc gctggataaa gtggcgatga aaatcaaacc
    45841 cgacgatatc catttcgccc gcaccaaatt actcagtctg gaaaacaccc acaacggcaa
    45901 agtgctgccg cgtgaatacc tgaaagaagc atgggaattt acccgcgagc gcaatctggc
    45961 gctgcatgtt gacggtgcgc gcatctttaa tgccgtggtg gcttacggct gcgaactgaa
    46021 agagatcacg caatattgtg attcgttcac catttgcctg tcgaaaggtc ttgggacgcc
    46081 agtcggttca ttactcgtcg gtaatcgtga ttacattaaa cgtgccatcc gctggcggaa
    46141 aatggccggt ggcgggatgc gtcagtccgg gattctggct gccgccggga tgtatgccct
    46201 gaaaaataac gtcgcacggt tgcaggaaga tcacgacaac gccgcctgga tggcggagca
    46261 actgcgtgaa gcaggcgcgg atgtgatgcg tcaggacact aatatgctgt ttgttcgcgt
    46321 cggggaagaa aatgctgccg cgttaggcga atacatgaaa gcgagaaacg tgctgattaa
    46381 cgcctcgccg attgtccgcc tggtgacgca tcttgacgtc tcgcgcgaac aactggcgga
    46441 agtcgccgcc cactggcgcg cattcctggc gcgttaagga gagagacgtg ccgcaacgca
    46501 ttttagttct cggtgccagt ggctacattg gtcagcatct ggtgcgcaca ctcagccagc
    46561 aagggcatca gatcctggcg gcggcacgtc atgtcgacag gcttgcaaag ctgcaactgg
    46621 caaacgtcag ttgccataaa gtcgatctca gctggccgga taaccttccg gccctgttgc
    46681 aggatatcga taccgtctat tttctggtgc acagcatggg cgaaggcggc gattttatcg
    46741 ctcaggagcg ccaggtggct ctcaacgtcc gcgatgcgct acgtgaagta ccagttaagc
    46801 aattaatctt tctcagttcg ttgcaggccc cgccacatga gcagtcggat catctgcgtg
    46861 ctcgtcaggc tacggcggac attcttcgtg aagcgaatgt acctgtgacc gaacttcggg
    46921 ccggaattat cgttggcgca ggttcagcgg cgttcgaagt catgcgcgat atggtctaca
    46981 acctgccagt gttaacgccg ccacgctggg tacgttcacg caccacgccc atcgcgctgg
    47041 aaaacttgct gcactatctg gtggcgttgt tagatcatcc ggccagcgaa caccgcatct
    47101 tcgaagccgc cggaccagag gtgctcagtt atcagcaaca gtttgaacat tttatggcgg
    47161 tgagcgg
//
LOCUS       DAZUFB010000028        44251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZUFB010000028 DAZUFB010000000
VERSION     DAZUFB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.028"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(79..1443)
                     /gene="mnmE"
                     /locus_tag="ACHY3M_001803"
     CDS             complement(79..1443)
                     /gene="mnmE"
                     /locus_tag="ACHY3M_001803"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4316734.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDANGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1549..3195)
                     /gene="yidC"
                     /locus_tag="ACHY3M_001804"
     CDS             complement(1549..3195)
                     /gene="yidC"
                     /locus_tag="ACHY3M_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4316735.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQHISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3198..3455)
                     /gene="yidD"
                     /locus_tag="ACHY3M_001805"
     CDS             complement(3198..3455)
                     /gene="yidD"
                     /locus_tag="ACHY3M_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4316736.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3419..3778)
                     /gene="rnpA"
                     /locus_tag="ACHY3M_001806"
     CDS             complement(3419..3778)
                     /gene="rnpA"
                     /locus_tag="ACHY3M_001806"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4316737.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3795..3935)
                     /gene="rpmH"
                     /locus_tag="ACHY3M_001807"
     CDS             complement(3795..3935)
                     /gene="rpmH"
                     /locus_tag="ACHY3M_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4316738.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4165..4245)
                     /locus_tag="ACHY3M_001808"
     CDS             complement(4165..4245)
                     /locus_tag="ACHY3M_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316739.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4542..5945
                     /gene="dnaA"
                     /locus_tag="ACHY3M_001809"
     CDS             4542..5945
                     /gene="dnaA"
                     /locus_tag="ACHY3M_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4316740.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILIKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5950..7050
                     /gene="dnaN"
                     /locus_tag="ACHY3M_001810"
     CDS             5950..7050
                     /gene="dnaN"
                     /locus_tag="ACHY3M_001810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4316741.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7050..8123
                     /gene="recF"
                     /locus_tag="ACHY3M_001811"
     CDS             7050..8123
                     /gene="recF"
                     /locus_tag="ACHY3M_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4316742.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8152..10566
                     /gene="gyrB"
                     /locus_tag="ACHY3M_001812"
     CDS             8152..10566
                     /gene="gyrB"
                     /locus_tag="ACHY3M_001812"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4316743.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10807..11205
                     /gene="yidB"
                     /locus_tag="ACHY3M_001813"
     CDS             10807..11205
                     /gene="yidB"
                     /locus_tag="ACHY3M_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFS4316744.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11320..12132
                     /gene="yidA"
                     /locus_tag="ACHY3M_001814"
     CDS             11320..12132
                     /gene="yidA"
                     /locus_tag="ACHY3M_001814"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4316745.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12178..12834)
                     /gene="yidX"
                     /locus_tag="ACHY3M_001815"
     CDS             complement(12178..12834)
                     /gene="yidX"
                     /locus_tag="ACHY3M_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFS4316746.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13112..13801
                     /gene="dgoR"
                     /locus_tag="ACHY3M_001816"
     CDS             13112..13801
                     /gene="dgoR"
                     /locus_tag="ACHY3M_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFS4316747.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13798..14676
                     /gene="dgoK"
                     /locus_tag="ACHY3M_001817"
     CDS             13798..14676
                     /gene="dgoK"
                     /locus_tag="ACHY3M_001817"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFS4316748.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14660..15277
                     /gene="dgoA"
                     /locus_tag="ACHY3M_001818"
     CDS             14660..15277
                     /gene="dgoA"
                     /locus_tag="ACHY3M_001818"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFS4316749.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15274..16422
                     /gene="dgoD"
                     /locus_tag="ACHY3M_001819"
     CDS             15274..16422
                     /gene="dgoD"
                     /locus_tag="ACHY3M_001819"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS4316750.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16497..17834
                     /gene="dgoT"
                     /locus_tag="ACHY3M_001820"
     CDS             16497..17834
                     /gene="dgoT"
                     /locus_tag="ACHY3M_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4316751.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17831..18895)
                     /gene="cbrA"
                     /locus_tag="ACHY3M_001821"
     CDS             complement(17831..18895)
                     /gene="cbrA"
                     /locus_tag="ACHY3M_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFS4316752.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCSGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            18996..20210
                     /gene="yidR"
                     /locus_tag="ACHY3M_001822"
     CDS             18996..20210
                     /gene="yidR"
                     /locus_tag="ACHY3M_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFS4316753.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20212..20544)
                     /gene="yidQ"
                     /locus_tag="ACHY3M_001823"
     CDS             complement(20212..20544)
                     /gene="yidQ"
                     /locus_tag="ACHY3M_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS4316754.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20784..20856
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20850..21263
                     /gene="ibpA"
                     /locus_tag="ACHY3M_001824"
     CDS             20850..21263
                     /gene="ibpA"
                     /locus_tag="ACHY3M_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFS4316755.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21375..21803
                     /gene="ibpB"
                     /locus_tag="ACHY3M_001825"
     CDS             21375..21803
                     /gene="ibpB"
                     /locus_tag="ACHY3M_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFS4316756.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22000..23661
                     /gene="yidE"
                     /locus_tag="ACHY3M_001826"
     CDS             22000..23661
                     /gene="yidE"
                     /locus_tag="ACHY3M_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFS4316757.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23658..24374)
                     /gene="yidP"
                     /locus_tag="ACHY3M_001827"
     CDS             complement(23658..24374)
                     /gene="yidP"
                     /locus_tag="ACHY3M_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4316758.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24670..26286
                     /locus_tag="ACHY3M_001828"
     CDS             24670..26286
                     /locus_tag="ACHY3M_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4316759.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLDIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26286..27100
                     /locus_tag="ACHY3M_001829"
                     /pseudo
     CDS             26286..27100
                     /locus_tag="ACHY3M_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27097..27990)
                     /gene="yidL"
                     /locus_tag="ACHY3M_001830"
     CDS             complement(27097..27990)
                     /gene="yidL"
                     /locus_tag="ACHY3M_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4316760.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRNKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28157..29872
                     /gene="yidK"
                     /locus_tag="ACHY3M_001831"
     CDS             28157..29872
                     /gene="yidK"
                     /locus_tag="ACHY3M_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS4316761.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29869..31362
                     /gene="yidJ"
                     /locus_tag="ACHY3M_001832"
     CDS             29869..31362
                     /gene="yidJ"
                     /locus_tag="ACHY3M_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4316762.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31409..31858)
                     /gene="yidI"
                     /locus_tag="ACHY3M_001833"
     CDS             complement(31409..31858)
                     /gene="yidI"
                     /locus_tag="ACHY3M_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFS4316763.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31968..32315
                     /gene="yidH"
                     /locus_tag="ACHY3M_001834"
     CDS             31968..32315
                     /gene="yidH"
                     /locus_tag="ACHY3M_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFS4316764.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32305..32667
                     /gene="yidG"
                     /locus_tag="ACHY3M_001835"
     CDS             32305..32667
                     /gene="yidG"
                     /locus_tag="ACHY3M_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFS4316765.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32664..33161
                     /gene="yidF"
                     /locus_tag="ACHY3M_001836"
     CDS             32664..33161
                     /gene="yidF"
                     /locus_tag="ACHY3M_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4316766.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33169..34353)
                     /gene="emrD"
                     /locus_tag="ACHY3M_001837"
     CDS             complement(33169..34353)
                     /gene="emrD"
                     /locus_tag="ACHY3M_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFS4316767.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34435..34509
                     /gene="ysdE"
                     /locus_tag="ACHY3M_001838"
     CDS             34435..34509
                     /gene="ysdE"
                     /locus_tag="ACHY3M_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFS4316768.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34772..34861)
                     /gene="tisB"
                     /locus_tag="ACHY3M_001839"
     CDS             complement(34772..34861)
                     /gene="tisB"
                     /locus_tag="ACHY3M_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFS4316769.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35426..35524
                     /gene="ivbL"
                     /locus_tag="ACHY3M_001840"
     CDS             35426..35524
                     /gene="ivbL"
                     /locus_tag="ACHY3M_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFS4316770.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35630..37318
                     /gene="ilvB"
                     /locus_tag="ACHY3M_001841"
     CDS             35630..37318
                     /gene="ilvB"
                     /locus_tag="ACHY3M_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFS4316771.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37322..37612
                     /gene="ilvN"
                     /locus_tag="ACHY3M_001842"
     CDS             37322..37612
                     /gene="ilvN"
                     /locus_tag="ACHY3M_001842"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS4316772.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37687..38277
                     /gene="uhpA"
                     /locus_tag="ACHY3M_001843"
     CDS             37687..38277
                     /gene="uhpA"
                     /locus_tag="ACHY3M_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFS4316773.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38277..39779
                     /gene="uhpB"
                     /locus_tag="ACHY3M_001844"
     CDS             38277..39779
                     /gene="uhpB"
                     /locus_tag="ACHY3M_001844"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFS4316774.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39789..41108
                     /gene="uhpC"
                     /locus_tag="ACHY3M_001845"
     CDS             39789..41108
                     /gene="uhpC"
                     /locus_tag="ACHY3M_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS4316775.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41364..42755
                     /gene="uhpT"
                     /locus_tag="ACHY3M_001846"
     CDS             41364..42755
                     /gene="uhpT"
                     /locus_tag="ACHY3M_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFS4316776.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42800..44249)
                     /gene="adeD"
                     /locus_tag="ACHY3M_001847"
                     /pseudo
     CDS             complement(42800..44249)
                     /gene="adeD"
                     /locus_tag="ACHY3M_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT        10186 a        11373 c        12027 g        10665 t
ORIGIN      
        1 agggttgagg aataacagga gtgctaacaa tgaagcgaag ccgcatctga cagtcagaat
       61 acggcttcgt aagcgcggtt acttaccaat acagaagctg gagaaaatcc gccccagcag
      121 gtcgtctgaa gtaaattccc cggtgatttc gcttaagttc tgctgtgcca ggcgcaactc
      181 ttccgccagc agttcacccg cccaggcacc taacagttgc gctttacctt gctgcaaatg
      241 ctccgctgcc tgttccagcg cctgtaagtg gcgacggcgc gccaggaaac cgccttccat
      301 gttggtgtca aagcccatgc tctgtttgag atggttacgc agcacgtcca cgccttcacc
      361 agtccttgcc gagagacgaa ttaacgcgtg accgttcact tcactcattc ccagcgtttc
      421 gccggtgata tcggctttat tgcgcaccac ggtgatcggc agtttcgctg gcagacgggc
      481 gataaattcc ggccagatct ctgccggatc cacggcgtct gttgtggtgc catcgaccat
      541 aaacagcacc cggtcggcct gttcaatttc ctgccacgcg cgctcgatcc caatacgttc
      601 cacttcgtca ctggcttcac gtagcccggc ggtgtcgatg atatgcagcg gcattccgtc
      661 aatgtggata tgctcacgca acacgtcacg cgtagttccg gcgatatcgg ttacgattgc
      721 tgcttcacgg ccagccagcg cgtttaacag gctcgattta ccggcgttag gacgtccggc
      781 aatcaccact ttcatccctt cgcgcaacaa actaccctga cgtgcttcag cacgcactgc
      841 atcaagatcg gcaataacgt cattgagctg ggcttcaatt ttgccgtcgg agaggaaatc
      901 gatctcttca tcagggaaat caattgccgc ttcgacgtaa atgcgcaagt gggtgagggc
      961 ttctaccaga tgattaaccc gtgcggagaa tgcgccttgc agcgagttaa gtgccgaacg
     1021 ggccgcctgt tccgagctgg cgtcgataag atcggcaatc gcctcagcct gggctaagtc
     1081 gagtttatcg taaagaaacg cgcgttcgga aaactcacca gggcgagcaa tccgcaggcc
     1141 gggaatggtc agaatgcgtt ttaacagcag gtcgaggatc accggaccgc catgaccctg
     1201 cagttcaagc acatcttcgc cggtgaacga gttcgggcca gggaaccata gcgcaatccc
     1261 ctgatcgagc acgctgccgt tggcgtcttt aaatggcaaa taatcggcgt agcgcggctt
     1321 aggtagttta cccagcacgg tttcggcaac ttcacgggct ttgaggccgg agatgcgcag
     1381 gatgccaaca ccgccacgtc ccggaggcgt ggcctgggct acgatagtgt cattatcgct
     1441 catgatgttc ctgttgcttt gtgtggcgga tgcgcggtgc ttatccgccc tacgaaaaga
     1501 aaaaaggcgg tcaactgacc gcccttattt tagcgaaaac tcaccgaatc aggatttttt
     1561 cttctcgcgg ctatgcaggc cacgtttttc cagaccacgg taaatcagct gctgctgaat
     1621 aatggttacc aggttgctga cgatatagta cagcaccaga cctgacggga accacaggaa
     1681 gaacacggtg aagatgaccg gcataaaggt catgatcttc tgctgcatcg ggtcggtcac
     1741 tgtggtcggc gacatcttct gaatgaagaa catcgttacg cccatcagga tcggcaggat
     1801 gtagtacggg tcctgtgccg acaggtcgtg gatccacagt gcaaacggtg cctgacgcag
     1861 ttcaacggaa cccatcagca tgtagtacaa cgccaggaag attggcatct ggatcagcag
     1921 cgggaagcag ccgcccagcg ggttaacctt ctcagctttg tacagcgcca tcatttcctg
     1981 gctgatatgc tgtttgtcat cgcccagacg ctcacgcatt gcctgaatct tcggctgcag
     2041 catacgcatc ttcgccatgg aggtgtactg cgctttggtc agcgggtaca tgatgccacg
     2101 aacgataaag gtgatgatga taatggagaa gccccagtta cccacaaagc tatggatcca
     2161 tttcagcagt ttgaacagcg gctgagagat gaaccacaac caaccgtaat caacggtcag
     2221 atccaggtgc ggagcaacag ctgccatttt gtcctggatt tccgggccaa cccacagggt
     2281 gctgttcatc gcgccagtct gaccaggctg aaccagtacc ggctgagatt tatagccgat
     2341 agcggcgatg ccgttaccca gattagcggt atagaagttg ttggtaccgt cgttatgcgg
     2401 gatccacgcc gtcgcgaaat actgttgcag catcgccacc caaccacctt tcgaagagat
     2461 gttcaggttt tcgttatcgg caatggtatc gaacttgtat ttctcatact tctcgtcagg
     2521 cgtggagtac gccgcgccac ggaaggtgtg cagtgcgaag ttgctgcttc cggtatcgag
     2581 atgcggtggc agagtgatgg attgcttcaa ctgaccaaag gtggagattt ccagcggttt
     2641 ctcgccagcg ttctgcacgt tgtagttgac gttgacagcg taatcaccac gtttcaggac
     2701 aaacgttttg gtaaacgtgt tgcctgccgc gtcggtatac gtcatcggca cctgcagttc
     2761 gttttgacct tcagccagca cataagcgtc tttttcaacg ttatacagcg gacgcgggcc
     2821 gttagccggg ttatccgggc catcacgacc ggtcagaccg ctctgtgcct gataaataaa
     2881 ctgcggtgaa gtttccagca gctggaacgg ctgggtagag ttcagctctt tcgggtaagc
     2941 aggcagcaga gcttgctcaa catcaccacc acgggtgttg atggtcagat caagcacgtc
     3001 ggtcttaacc gagatcagtt tcccctggcc actggccggt acgccctggt cggcggcgct
     3061 acccgctgcg gtggtcgttg tctgcgtggt ctgttgggcc tgaggttgcg ggtttttatc
     3121 ctgctcccag gcttgccaga tcatgaaaga cacgaacagc aaagcgatga ctaaaagatt
     3181 gcgttgcgaa tccatcgtta gtgttctctg gtatcaaatg gtccgggcgg gacgggatcg
     3241 tcaccaccag ggtgtaaagg gtggcatttt aatacgcgtt tcaccgtcaa ccaactgcct
     3301 tttatcactc caaacctgcg caatgcctca attccgtagc ttgaacaggt tggagtgaaa
     3361 cgacaatgcg gcccgagtag cggactaatc aggcgttgat agacccgaat gagggctatc
     3421 aggacccgcg agccaggcga cagtggcggc gccataattt ttccaacgct tccgagagag
     3481 cacggttatc gaggtcggca accccttttt tcgccaccac cacgaaatcc atagccggga
     3541 gttcatgttg gcgcagacgg aagctttcac gcgtcagacg tttaatccga ttgcgttcat
     3601 gggcgcgtcg aacgtttttc ttggcgactg taagaccgat acggggatgc cccagcgaat
     3661 tcaggcggcc gagaatggta atttgcggcg tgccagcccg ttgtggctgc tggaagacga
     3721 atgtgaattg actgggagtt aacaagcgta actccctggg aaatgcgagc ttaaccactc
     3781 aggggttagc tttattactt agaaacggtc agacgagcgc ggcctttagc acgacgacgt
     3841 gccagaacct gacgaccatt tttagtagcc atacgagcac ggaagccgtg agaacggttg
     3901 cgcttcagta cagacggttg aaaagtgcgt ttcatggcga tttctaccta aacttgaata
     3961 aattcaatgg ctttattgga tatccgccga aaaatgaaac gatggacacc gaagccatgg
     4021 gtgattaaag aggccggatt gtaataattg tacactccgg agtcaattct ctttccttat
     4081 ttaccgcgct tttccgcacc ttttcgcagg gaaaatgtac gacctcacac cagtgaaaac
     4141 cagcatggcg cgccgggtgg aggattatac gggctgatgg gtaaagcgca aggatcgtcc
     4201 tggatcttta ttagatcgat taagccaatt tttgtctatg gtcattaaat tttccaatat
     4261 gcggcgtaaa tcgtgcccgc ctcgcggcag gatcgtttac acttagcgag gtctggaaag
     4321 tcctgtggat aaatcgggaa aatctgtgag aaacagaaga tctcttgcgc agtttaggct
     4381 atgatccgcg gtcccgatcg ttttgcagga tcttgatcgg gcatataacc gcagacagcg
     4441 gttcgtgcgt caccctcaag cagggtcttt tcgacgtacg tcaacaatca tgaatgtttc
     4501 agccttagtc attatcgact tttgttcgag tggagtccgc cgtgtcactt tcgctttggc
     4561 agcagtgtct tgcccgattg caggatgagt taccagccac agaattcagt atgtggatac
     4621 gcccattgca ggcggaactg agcgataaca cgctggccct gtacgcgcca aaccgttttg
     4681 tcctcgattg ggtacgggac aagtacctta ataatatcaa tggactgcta accagtttct
     4741 gcggagcgga tgccccacag ctgcgttttg aagtcggcac caaaccggtg acgcaaacgc
     4801 cacaagcggc agtgacgagc aacgtcgcgg cccctgcaca ggtggcgcaa acgcagccgc
     4861 aacgtgctgc gccttctacg cgctcaggtt gggataacat cccggccccg gcagaaccga
     4921 cctatcgttc taacgtaaac gtcaaacaca cgtttgataa cttcgttgaa ggtaaatcta
     4981 accaactggc gcgcgcggcg gctcgccagg tggcggataa ccctggcggt gcctataacc
     5041 cgttgttcct ttatggcggc acgggtctgg gtaaaactca cctgctgcat gcggtgggta
     5101 acggcattat ggcgcgcaag ccgaatgcca aagtggttta tatgcactcc gagcgctttg
     5161 ttcaggacat ggttaaagcc ctgcaaaaca acgcgatcga agagtttaaa cgctactacc
     5221 gttccgtaga tgcactgctg atcgacgata ttcagttttt tgctaataaa gaacgatctc
     5281 aggaagagtt tttccatacc ttcaacgccc tgctggaagg taatcaacag atcattctca
     5341 cctcggatcg ctatccgaaa gagatcaacg gcgttgagga tcgtttgaaa tcccgcttcg
     5401 gttggggact gactgtggca atcgaaccgc cagagctgga aacccgtgtg gcgatcctga
     5461 ttaaaaaggc cgacgaaaac gacattcgtt tgccgggcga agtggcgttc tttatcgcca
     5521 agcgtctacg atctaacgta cgtgagctgg aaggggcgct gaaccgcgtc attgccaatg
     5581 ccaactttac cggacgggcg atcaccatcg acttcgtgcg tgaggcgctg cgcgacttgc
     5641 tggcattgca ggaaaaactg gtcaccatcg acaatattca gaagacggtg gcggagtact
     5701 acaagatcaa agtcgcggat ctcctttcca agcgtcgatc ccgctcggtg gcgcgtccgc
     5761 gccagatggc gatggcgctg gcgaaagagc tgactaacca cagtctgccg gagattggcg
     5821 atgcgtttgg tggtcgtgac cacacgacgg tgcttcatgc ctgccgtaag atcgagcagt
     5881 tgcgtgaaga gagccacgat atcaaagaag atttttcaaa tttaatcaga acattgtcat
     5941 cgtaaaccta tgaaatttac cgtagaacgt gagcatttat taaaaccgct acaacaggtg
     6001 agcggtccgt taggtggtcg tcctacgcta ccgattctcg gtaatctgct gttacaggtt
     6061 gctgacggta cgttgtcgct gaccggtact gatctcgaga tggaaatggt ggcacgtgtt
     6121 gcgctggttc agccacacga gccaggagcg acgaccgttc cggcgcgcaa attctttgat
     6181 atctgccgtg gtctgcctga aggcgcggaa attgccgtgc agctggaagg tgaacggatg
     6241 ctggtacgct ccgggcgtag ccgtttttcg ctgtctaccc tgccagcggc ggatttcccg
     6301 aacctcgatg actggcagag tgaagtcgaa tttaccctgc cgcaggcaac gatgaagcgt
     6361 ctgattgaag cgacccagtt ttcgatggcg catcaggacg ttcgctatta cttaaatggt
     6421 atgctgtttg aaaccgaagg tgaagaactg cgcaccgtgg caaccgacgg ccaccgtctg
     6481 gcggtctgtt caatgccaat tggtcaatct ttgccaagcc attcggtgat cgtaccgcgt
     6541 aaaggcgtga ttgaactgat gcgtatgctc gacggcggcg acaatccgct gcgcgtgcag
     6601 attggcagca acaatattcg cgcccacgtt ggcgacttta tcttcacctc caaactggtg
     6661 gatggtcgct tcccggatta ccgccgcgtt ctgccgaaga atccggacaa acatctggaa
     6721 gctggctgcg atctgctcaa gcaggcgttt gcccgtgcgg caattctctc taacgagaaa
     6781 ttccgcggcg tgcgcctgta tgtcagcgaa aaccagctga aaatcaccgc caacaacccg
     6841 gaacaggaag aagcggaaga gatcctcgac gttacctata gcggtgcgga gatggaaatc
     6901 ggcttcaacg tcagctatgt gctggatgtt ctgaacgcgc tgaaatgcga aaacgtccgc
     6961 atgatgctga ccgattcggt ttccagcgtg cagattgaag atgccgcatc acagtcggct
     7021 gcctatgttg tcatgccaat gagactgtaa tgtccctcac ccgcttgttg atccgcgatt
     7081 tccgcaacat tgaaaccgcg gatctcgctt tatctcccgg ctttaacttt ctggtaggtg
     7141 ccaacggcag tggcaaaacc agcgtgctgg aagccatcta tacgctcggc catggtcggg
     7201 cgtttcgcag tttgcagatt ggtcgcgtca ttcgccatga gcaggaggca tttgttctcc
     7261 atgggcgatt acagggcgaa gagcgcgaga cggcgattgg cttaaccaag gacaaacagg
     7321 gcgacagcaa agtccgcatc gacggtactg acgggcataa agtcgcggaa ctggcgcacc
     7381 tgatgccaat gcagctgata acgccagaag ggtttacttt actcaacggc ggccccaaat
     7441 acagaagagc attcctcgac tggggatgct ttcacaacga acccggattt ttcaccgcct
     7501 ggagcaatct caagcgattg ctcaagcagc gcaatgcggc gctgcgccag gtgacacgtt
     7561 acgaacagct acgcccgtgg gataaagaac tgatcccgct ggcggagcaa atcagcacct
     7621 ggcgcgcgga gtatagcgcc ggtatcgcgg ccgatatggc cgatacctgt aagcaatttc
     7681 tccctgagtt ttctctgact ttctctttcc agcgcggctg ggagaaagag acagaatatg
     7741 ctgaggtgct ggaacgtaat tttgaacgcg atcgccagct aacctacacc gcgcatggcc
     7801 cgcataaagc ggacttacgc attcgcgccg acggtgcgcc ggtggaagat accttatcgc
     7861 gtgggcagct taagctgttg atgtgcgcct tacgtctggc gcaaggagag ttcctcaccc
     7921 gtgaaagcgg gcggcggtgt ctctacctga tagatgattt tgcctctgag cttgatgatg
     7981 agcgtcgtgg gttgcttgcc agccgcttaa aagcgacgca atcacaggtc tttgtcagcg
     8041 cgatcagtgc tgaacacgtt atagacatgt cggacgaaaa ttcgaagatg tttaccgtgg
     8101 aaaagggtaa aataacggat taacccaagt ataaatgagc gagaaacgtt gatgtcgaat
     8161 tcttatgact cctccagtat caaagtcctg aaagggctgg atgcggtgcg taagcgcccg
     8221 ggtatgtata tcggcgacac ggatgacggc accggtctgc accacatggt attcgaggtg
     8281 gtagataacg ctatcgacga agcgctcgcg ggtcactgta aagaaattat cgtcaccatt
     8341 cacgccgaca actctgtctc tgtacaggat gacgggcgcg gcattccgac cggtattcac
     8401 ccggaagagg gcgtatcggc ggcggaagtg atcatgaccg ttctgcacgc aggcggtaaa
     8461 ttcgacgata actcctataa agtgtccggc ggtctgcacg gcgttggtgt ttcggtagta
     8521 aacgccctgt cgcaaaaact ggagctggtt atccagcgcg agggtaaaat tcaccgtcag
     8581 atctacgaac acggtgtacc gcaggccccg ctggcggtta ccggcgagac tgaaaaaacc
     8641 ggcaccatgg tgcgtttctg gcctagcctc gaaactttca ccaatgtgac cgagttcgaa
     8701 tatgaaattc tggcgaaacg tctgcgtgag ttgtcgttcc tcaactccgg cgtttccatt
     8761 cgtctgcgcg acaagcgcga cggcaaagaa gaccacttcc actatgaagg cggcatcaag
     8821 gcgttcgttg aatatctgaa caagaacaaa acgccgatcc acccgaatat cttctacttc
     8881 tccactgaaa aagacggtat tggcgtcgaa gtggcgttgc agtggaacga tggcttccag
     8941 gaaaacatct actgctttac caacaacatt ccgcagcgtg acggcggtac tcacctggca
     9001 ggcttccgtg cggcgatgac ccgtaccctg aacgcctaca tggacaaaga aggctacagc
     9061 aaaaaagcca aagttagcgc caccggtgac gatgcgcgtg aaggcctgat tgcggtcgtt
     9121 tccgtgaaag tgccggaccc gaaattctcc tcccagacca aagacaaact ggtttcttct
     9181 gaggtgaaat cagcggttga acagcagatg aacgaactgc tggcagaata cctgctggaa
     9241 aacccaaccg acgcgaaaat cgtggttggc aaaattatcg atgctgcccg tgcccgtgaa
     9301 gcggcgcgtc gcgcgcgtga aatgacccgc cgtaaaggtg cgctcgactt agcgggcctg
     9361 ccgggcaaac tggcagactg ccaggaacgc gatccggcgc tttccgaact gtacctggtg
     9421 gaaggggact ccgcgggcgg ctctgcgaag caggggcgta accgcaagaa ccaggcgatt
     9481 ctgccgctga agggtaaaat cctcaacgtc gagaaagcgc gcttcgataa gatgctctct
     9541 tctcaggaag tggcgacgct tatcaccgcg cttggctgtg gtatcggtcg tgacgagtac
     9601 aacccggaca aactgcgtta tcacagcatc atcatcatga ccgatgcgga cgtcgacggc
     9661 tcgcacattc gtacgctgct gttgaccttc ttctatcgtc agatgccgga aatcgttgaa
     9721 cgcggtcacg tctacatcgc tcagccgccg ctgtacaaag tgaagaaagg caagcaggaa
     9781 cagtacatta aagacgacga agcgatggat cagtaccaga tctctatcgc gctggatggc
     9841 gcaacgctgc acaccaacgc cagcgcaccg gcattggctg gcgaagcgtt agagaaattg
     9901 gtgtctgagt acaacgcgac gcagaaaatg atcaaccgca tggagcgtcg ttatccgaaa
     9961 gcaatgctga aagagcttat ctatcagccg acgctgacgg aagccgacct ctctgatgag
    10021 cagaccgtta cccgctgggt gaacgcgctg gtcagcgaac tgaacgacaa agaacagcac
    10081 ggcagccagt ggaagtttga tgtccacacc aatgccgaac aaaacctgtt cgagccgatt
    10141 gttcgcgtgc gtacccacgg tgtggatact gactatccgc tggatcacga gtttatcacc
    10201 ggtggcgaat atcgtcgtat ctgcacgctg ggtgagaaac tgcgtggctt gctggaagaa
    10261 gatgcgttta tcgaacgtgg cgagcgtcgt cagccggtag ccagcttcga gcaggcgctg
    10321 gactggctgg tgaaagagtc ccgtcgcggc ctctccatcc agcgttataa aggtctgggc
    10381 gagatgaacc cggaacagct gtgggaaacc accatggacc cggaaagccg tcgtatgctg
    10441 cgcgttaccg ttaaggatgc gattgccgct gaccagttgt tcaccacgct gatgggcgac
    10501 gccgttgaac cgcgccgtgc gtttattgaa gagaacgccc tgaaagcggc gaatatcgat
    10561 atttaatggc gctaaccatg cgagcgtgcc tgatgcgcta cgcttatcag tatctacgaa
    10621 aatcttgcaa tgtattgaat tttcatgatt ttgtaggccg gataaggcgt tcacgccgca
    10681 tccggcaaga agctataaga aaagggcgga gatcatctcc gcccttttta tttctgcaat
    10741 ccgcaattat catcccgcaa atctatactt tttgggtcaa cacgtaacgc tttacaggtg
    10801 attatcatgg ggctttttga tgaagttgtc ggtgcctttc tgaaaggcga tgcggggaaa
    10861 tatcaggcta ttttaagttg ggttgaggag cagggcggca ttcaggtgct gctggaaaaa
    10921 ctgcaaagtg gcggcttagg ggccattctc tcaacctggc tgagtaatca acagggcaat
    10981 caatcggtta gtggcgagca actggaatcg gcgctcggca caaatgcggt gtccgatctt
    11041 ggacaaaaac ttggcgtgga taccagtaca gcttccagtt tactggcaga acaattgccg
    11101 aagattattg atgcgctctc accgcaaggt gaagtgtctc cacaagccaa taacgatctg
    11161 ctttccgcag gcatggaact gctgaaaggg aaactcttcc gctaagcaaa tggggagcat
    11221 gcaacccgcg ttgcatcccc cttttccctc gggtgttgta ctgatttttg agcggaatcg
    11281 cgttagcatg ggtcaggaac taatctacct ggggaactca tggctatcaa actcattgct
    11341 atcgatatgg atggcaccct tctgctaccc gatcacacca tttcacccgc cgttaaaaat
    11401 gcgattgccg cagctcgcgc ccgtggcgtg aatgtcgtgc taacgacggg tcgcccttat
    11461 gcaggtgttc acaattacct gaaagagctg catatggaac tgccgggcga ctactgcatt
    11521 acttataacg gcgcgctggt acagaaggcc gctgatggta gcaccgtggc gcaaaccgct
    11581 ctcagctatg acgactaccg tttcctggaa aaactctctc gcgaagtcgg ttctcatttc
    11641 cacgccctgg accgcaccac gctgtacacc gccaaccgtg atatcagcta ctacacggtg
    11701 catgaatcct tcgttgccac aattccgctg gtgttctgcg aagcggagaa aatggacccc
    11761 aatacccagt tcctgaaagt gatgatgatt gatgaacccg ccatcctcga ccaggctatc
    11821 gcgcgtattc cgcaggaagt gaaagagaaa tataccgtgc tgaaaagtgc gccgtacttc
    11881 ctcgaaatcc tcgataaacg cgttaacaaa ggtacggggg tgaaatcact ggccgacgtg
    11941 ttaggtatta aaccggaaga aatcatggcg attggcgatc aggaaaacga catcgcaatg
    12001 attgaatatg caggcgtcgg tgtggcgatg gataacgcta ttccttcggt gaaagaagtg
    12061 gcgaactttg tcaccaaatc caaccttgaa gatggcgtgg cgtttgctat tgagaagtat
    12121 gtgctgaatt aatcagtgga cgggcaaaca gcataaacgc gtttgcccgc ttactgatta
    12181 ttggtttact gcatatacaa ccagaaattg cagcggtgca gtaatgatgt ccagcgtaac
    12241 agtcactggc agtaatgctg cggaaacacc atccggaatg cctctgggtg aatagtattc
    12301 ataaaatcgc actttaaacg gatgatagaa cgccatcacc tttgagtaat ccatgttttt
    12361 atttttctca tggattgtgc cttttaggtt ttccagtgac ctggtacagt gttgaacacc
    12421 acaggcaaaa ccataatgcg ttgccagtgc cttttcttct tcgttattcc agtggtagta
    12481 aagcgatatt gtgccggtaa atttcttttt ccccgcattt aaaacaaatc ttgcgtcatc
    12541 ggcaacctga atattgtgcc ggttaagtgc tggatcattg agcatcttaa cgatgtcatc
    12601 ggcaccatca gtgataaggt aatccagcga ttcccctaca aaaacatagc cttttgttcc
    12661 attactatct tttgcctgcg atagcccagt gatggtgtct gactgcgatt gatactttcc
    12721 tgaactaccc tttgcggtat gggaaaccag agcatacgag atacagcctg aaagcatcag
    12781 cgccagtggg aataaaccgg cagccttaaa aaatccctta aaattcaact tcatgattaa
    12841 tcctttaaac gagtagctaa gccgttgaaa gcatactctt ctttccctcc tgtttagcgc
    12901 ctgatatccc ttttcagatt tctgcccgac gcatgtcatt tttttatgca ttgttctttt
    12961 tgtgatctaa attgtagtac aacaatataa gtttgtacta cattacgcac acggcaaacg
    13021 cgaacgtcat cacgctggta ctacaaagtt gccgcgttat gcatcgatcg gggtaaagta
    13081 gagaagaaca tacagagcac aaggactctc catgactctc aataaaaccg atcgcattgt
    13141 cattacgctg ggtaaacaga tcgttcacgg caaatacgta cctggctcgc cacttccggc
    13201 tgaggcggag ctctgtgaag agtttgcaac ctcgcgcaac atcatccgtg aggtgttccg
    13261 ttcgttgatg gcgaaacggc tgattgaaat gaaacgttat cgcggcgcgt ttgtggcacc
    13321 gcgtaaccag tggaattacc tcgacactga cgtactgcaa tgggtgctgg aaaacgacta
    13381 cgacccacgg cttatcagtg ccatgagcga agtgcgaaat ctggtggaac cggcgattgc
    13441 ccgttgggca gcagagcgcg cgacttccag cgatctggcg cagattgaat cggcgctgaa
    13501 cgagatgatt gccaacaatc aggaccgcga agcgtttaac gaagcggata ttcgctacca
    13561 cgaggcggtg ctgcagtcgg tacataaccc ggtgttacag caacttagca ttgcgatcag
    13621 ttcgttgcag cgggcggttt ttgaacgaac ctggatgggc gatgaggcca acatgccgca
    13681 aacgctccag gaacataagg cgctgttcga tgcaatacgg catcaggacg gcgatgcggc
    13741 agagcaggcg gcgcttacca tgatcgccag ctcgacacga aggttaaagg aaatcacatg
    13801 acagctcgct acatcgcaat tgactgggga tcgaccaatc ttcgcgcctg gctttatcag
    13861 ggcgaccact gcctggagag caggcaatca gaagcaggcg tcacgcgcct gaacggaaaa
    13921 tctccggctg cggtgttagc agaagtcacg accgactggc gtgaagagaa tacgccagtg
    13981 gtaatggcag gaatggtcgg cagcaatgtc ggctggaaag ttgctccgta tttatctgtt
    14041 cctgcccgtt tttcgtctat tggcgaacaa ttaacgtctg ttggcgacaa tatctggatt
    14101 attcccggat tatgcgtctc tcatgacgat aaccacaatg tgatgcgcgg cgaagaaaca
    14161 caattgatcg gcgcgcgaac tctggctcct tcctctcttt atgttatgcc cggtacgcat
    14221 tgcaaatggg tgcaggccga tagccagcaa atcaacgatt ttcgcaccgt gatgaccggt
    14281 gaattacatc atttactgtt aaatcactca ttgattggcg caggtttgcc gccgcaggaa
    14341 aactctgccg atgccttcgc ggctggcctt gagcgcggcc ttaatgcgcc cgccatattg
    14401 ccgcagcttt ttgaagttcg cgcctcgcat gtgctgggaa cacttccccg cgaacaggtc
    14461 agcgaatttc tctctggttt gttgattggc gcagaggttg ccagtatgcg cgactatgtg
    14521 acccatcaac acgccatcac ccttgtcgcc ggaacatcgc tgaccgcgcg ctaccagcaa
    14581 gcctttcagg cgatgggttg cgacgtgacg gcggtggcgg gcgacacggc atttcaggct
    14641 ggtataagga gcatcgctca tgcagtggca aactaaactt ccgctgatcg ccattttgcg
    14701 cggcattaag cccgacgagg cgctggcgca tgttggcgcg gtgattgacg ccgggttcga
    14761 cgcggttgaa atcccgctga attccccaca atgggagcaa agcattcccg ccatcgttga
    14821 tgcgtatggc gacaaggcgt tgattggcgc aggtacggta ctgaaacctg aacaggtcga
    14881 tgcgctcgcc aggatgggtt gtcagctcat cgttacgccc aatatccata gtgaagtgat
    14941 ccgccgtgcg gtgggctacg gcatgaccgt ctgccccggc tgcgcgacgg cgaccgaagc
    15001 ctttaccgcg ctcgaagcgg gcgcgcaggc gctgaaaata tttccgtcat cggcttttgg
    15061 tccgcaatac atcaaagcgt taaaagcggt attgccatcg gacatcgcag tctttgccgt
    15121 tggcggcgtg acgccagaaa acctggcgca gtggatagac gcaggttgtg ctggggcggg
    15181 cttaggcagc gatctctatc gcgccgggca gtccgtagaa cgcaccgcgc agcaggcagc
    15241 agcatttgtt aaggcgtatc gagaggcagt gcaatgaaaa tcaccaaaat taccacgtat
    15301 cgtttacctc cccgctggat gttcctgaaa attgaaaccg atgaaggcgt ggtcggttgg
    15361 ggcgagcccg tgattgaagg ccgcgcccgt acggtggaag cggcagttca cgagctgggt
    15421 gactatttga ttggtcagga tccttcgcgc atcaatgact tatggcaagt gatgtatcgc
    15481 gccggatttt atcgtggcgg tccaatcctg atgagcgcca ttgccgggat cgaccaggcg
    15541 ttatgggata tcaaaggcaa agtgctgaat gcgccggtct ggcaactgat gggcggcctg
    15601 gttcgcgaca aaattaaagc ctacagttgg gtcggcggcg atcgtccggc ggatgttatc
    15661 gacggcatta aaaccctgcg cgaaatcggc ttcgatacct tcaaactgaa cggttgtgaa
    15721 gaactggggc taattgataa ctcccgcgcg gtagatgcgg cagtcaacac cgtggcacaa
    15781 attcgtgaag cttttggcaa tcagattgag tttggtcttg atttccacgg tcgcgtcagc
    15841 gcgccaatgg cgaaagtgct gattaaagaa ctggagccgt atcgcccgct gtttattgaa
    15901 gagccggtgc tggcggaaca agccgaatac tacccgaaat tggcggcaca aacgcatatt
    15961 ccactggcgg caggtgagcg catgttctca cgtttcgatt ttaaacgcgt gctggaggca
    16021 ggcggtattt cgattctgca accggatctc tcccatgcag gcggtattac cgaatgctac
    16081 aaaattgctg gaatggcaga agcctatgat gtgacccttg cgccgcactg tccgctcgga
    16141 ccgattgcac tggcggcttg cctgcatatc gactttgttt cctataacgc ggtacttcag
    16201 gaacaaagta tgggcattca ttacaacaaa ggcgcggagt tactcgactt tgtgaaaaac
    16261 aaagaagact tcagtatggt tggcggcttc tttaaaccgt taacgaaacc gggcttaggt
    16321 gtggaaatcg acgaagctaa agttattgag ttcagtaaaa atgccccgga ctggcgtaat
    16381 ccgctctggc gtcatgaaga taacagcgta gcagagtggt aattcctgcc acgtaagccc
    16441 ctcatcgggc actaaaacag caatacaaaa atataaccct ctgtaaatta cagggcatgg
    16501 tgagcggctt cgctatgccc aaaatctgga gaaagctcgc tatggatatt cccgttaatg
    16561 cagcaaagcc ggggcgtcgg cgttatctga cgctggtgat gatctttatt acggtggtca
    16621 tttgttatgt tgaccgcgct aacctggccg tggcttccgc ccatattcag gaagagttcg
    16681 gcattacgaa agcggaaatg ggctatgtat tttcggcctt cgcctggctt tatacgctat
    16741 gccagatccc cggcggttgg tttttagatc gcgtaggttc tcgcgtgact tattttattg
    16801 cgatatttgg ttggtcagtg gcgactttat tccagggctt tgccacggga ttaatgtcat
    16861 taattggtct gcgtgcgata accggtattt tcgaagcgcc cgctttcccg accaataacc
    16921 ggatggtgac cagctggttc ccggaacatg aacgcgcttc cgccgttggt ttttatacgt
    16981 ctggtcagtt tgtcggtctg gcgtttttga ctccgctgct gatctggatt caggagatgt
    17041 tgagctggca ctgggtgttc attgtcactg gtggtatcgg cattatctgg tcgctgattt
    17101 ggtttaaggt ttatcagccg ccgcgcctga ccaaaggtat cagcaaagct gaactggatt
    17161 acattcgtga tggcggcggt ctggtggatg gtgatgcgcc ggtgaagaaa gaggcgcgtc
    17221 agccgttaac agccaaagac tggaaactgg tgttccatcg taaactgatc ggcgtttatc
    17281 ttgggcaatt tgcggtggct tctacactgt ggtttttctt aacctggttc ccgaactatt
    17341 taacccagga aaaaggaatc acggcgctga aagcaggctt tatgaccacg gtgccattcc
    17401 tcgcggcgtt tgtcggcgtc ctgctctctg gctgggtcgc ggatctgctg gtacgtaagg
    17461 gcttttcact gggctttgcg cgtaaaacgc cgattatctg cggcttgctg atctccacct
    17521 gcattatggg cgctaactac accaacgatc cgatgatgat tatgtgcctg atggcgctgg
    17581 cattcttcgg caacggtttt gcttcgatta cctggtcgct ggtctcttct ctggcaccga
    17641 tgcgcctgat tggtttaacc ggcggcgtgt ttaacttcgc cggtggtctg ggcggcatca
    17701 ccgttccgct ggtggtgggg tacctggcgc agggttacgg tttcgcacct gcactggttt
    17761 atatctccgc cgtcgcgttg attggcgcgc tctcttacat cctgctggtg ggcgatgtga
    17821 agcgcgttgg ctaatctttc aactgtggaa tatgcgccat accactgcgc ataatccgct
    17881 ttcttaaagc cggtgcggtc aggcatcggc tttttactat cttgccgaag agttttaaac
    17941 gcagtttgcg ggtggcgcgc cagtaagcgg tattgagctt ctctggctgc ttcagtaaca
    18001 ccgaacgcag aatgtctgcg ctatccagcg catagctaat cccctccagc gaactggcgc
    18061 tgataaatcc tgccgcttcg ccaatcagaa aggcgttgtc cttaccgcag acaaaatcct
    18121 gccagcgtga gggaaacagc accgtgcatt tttcgctttt caccgcctta ccaaactgga
    18181 actgaaaggc gctcattttc tctttcagcg tcgtgaaacg cgtctgaccg tctttcattg
    18241 gataagcacc gccaaagata aaataaccgt ctttgctgat actccatgaa taacagttag
    18301 ttatcgaatt atcaaagatg caggagtaga acggcaccgg atgtttctct gcgaaccact
    18361 gctggatagc gacatattta cggatttgat gatccgggta gagatgtcgc cgcaccatcg
    18421 agtttgcgcc atcggcaccg accagatagc gggcagtaat atgttgctcc cagccgtctg
    18481 cacgaaaaat gacatgccat ttatcatcct cacgccagat ttttcgacac aggctgtcgt
    18541 gataaacctc aacgctggcg gggatcagcg atttcatcca caagtcgaaa gcatggcgat
    18601 taatattgat atagcttcgc tggtagttac gcgtgagcga tgcggcgacg tcgacagttt
    18661 tgacgctgaa aatctgcggg ttggcgatca catcgacagg aagcgtcagt ccatcgcgaa
    18721 taaaagatcg ctgcgcgtcc ggtgccagca gaccgctgca aggtttgctg aaaccttcag
    18781 taccacactg gtgcttttta tccagcgcga tcacctgcat tttgcctgct aattttcgcg
    18841 ccaacgccga tcctgccggg ccgaggccaa taatcgccac gtcgaaatgt tccatctttt
    18901 cactcacatt catcacgatg gcaaaagact attgtctgaa agtgaagtcc ttgtgaagta
    18961 tgatggcggg acccgttttg tatcaggacc gcgccatgaa acagataacc tttgctcccc
    19021 gtaatcacct gctcaccaat accaatacct ggacgcccga cagccagtgg ctggtatttg
    19081 acgtgcgtcc ttctggggcg tcgtttaccg gcgagaccat tgagcgtgtg aatatccata
    19141 ccggcgaggt cgaggttatc tatcgcgcgt cacagggcgc acacgtcggc gtggtgaccg
    19201 ttcatccgaa gtcagagaaa tatgttttca tccatggccc ggaaaatcct gatgaaacat
    19261 ggcattacga tttccatcat cgacgcggag tcattgctga aggcggcaag gtgagcaatc
    19321 tcgatgcaat ggatattacc gcgccgtata tgccaggagc gctgcgcggc ggcagccatg
    19381 tgcatgtctt tagcccgaac ggtgaaaggg tgagctttac ctataacgac catgtaatgc
    19441 atgaactcga tccggcgctg gatttgcgaa acgtcggtgt tgctgcgccg tttggcccgg
    19501 tcaacgtaca aaagcagcat ccgcgtgaat acagcggtag ccactggtgc gtgctggtga
    19561 gtaaaaccac gcccacgccg cagcctggca gcgatgaaat caatcgtgct tatgaagaag
    19621 gatgggtagg aaatcacgcg ctggcgttta ttggcgacac actttcgcca aagggcgaga
    19681 aagtgccgga gctgtttatc gttgagttac cgcaagatga agctggctgg aaagcggcag
    19741 gtgatgcgcc gttaagtgga acggaaacca ccctgcccgc gccaccgcgc ggcgtcgtgc
    19801 agcgacgttt aacctttacc caccatcggg cttatccggg gttagtcaac gtcccgcgcc
    19861 actgggtgcg ctgtaatccg cagggtacgc aaatcgcgtt tttaatgcgt gatgataacg
    19921 gcattgtgca actgtggctt atctcgccac agggcggcga gccgcgccag ttaacccata
    19981 acaaaacgga tattcagtct gcatttaact ggcatccgtc aggagaatgg ttgggctttg
    20041 tgctggataa tcgaattgct tgtgcccatg cgcaaagcgg cgaggttgag tatttaaccg
    20101 aaaaccacgc caatccacct tctgcggacg ccgaggtctt ctcgccggat ggtcaatggc
    20161 tggcgtggat ggaaggtggc cagctgtgga tcaccgaaac tgatcgctaa atcagttatc
    20221 aggcattctg gccggtggaa taaccgcatt ggtggcttgc gcgttggctt cgcttttttc
    20281 aacgcgtgag cgcaccgagc tgtctttgcg gaacacatcc cacggcagta aaagcgtgtc
    20341 catcacagcg gtaaacggca tatcgagaat cgccagcgac ttagtgcccc agttggtttc
    20401 atcgtcgcca atcatggttg cgctggcgcg ggtgccggga tacgttcctt ctttccctcc
    20461 ggtatgggac atcacgctgg agcatcctcc gagtaatgtc attccgctgc atatcatgaa
    20521 cgctaacaac acatttctta tcataataaa ttcatctgtt gatcgtgggt gttggcctga
    20581 tgagttatag cgatcccttg ctgaaaataa catcatcatt acgtcgcact gtggcggcta
    20641 tcgcacttta acgtttcgtg ctgccccctc agtctatgca atagaccata aactgcaaaa
    20701 aaaagtccgc tgataaggct tgaaaagttc atttccagac ccatttttac atcgtagccg
    20761 atgaggacgc gcctgatggg tgttctggct acctgacctg tccattgtgg aaggtcttac
    20821 attctcgctg atttcaggag ctattgatta tgcgtaactt tgatttatcc ccgctttacc
    20881 gttctgctat tggatttgac cgtttgttta accacttaga aaacaaccag agccagagta
    20941 atggcggcta ccctccgtat aacgttgaac tggtagacga aaaccattac cgcattgcta
    21001 tcgctgtggc tggttttgct gagagcgaac tggaaattac cgcccaggat aatctgctgg
    21061 tggtgaaagg tgctcacgcc gacgaacaaa aagagcgcac ctatctgtac cagggcatcg
    21121 ctgaacgcaa ctttgaacgc aaattccagt tagctgagaa cattcatgtt cgtggcgcta
    21181 acctggtaaa tggtttgctg tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac
    21241 cgcgccgtat cgaaatcaac taattcccta aggccgcctg gcgcggcctg acatctccat
    21301 gctcgccgtc agggagcata tgcgaatctt cggatttgca ggtacttact cgcttcttag
    21361 aaggagaaat gactatgcgt aacttcgatt tatccccact gatgcgtcaa tggatcggtt
    21421 ttgacaaact ggccaacgca ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca
    21481 acattgagaa aagcgacgat aaccactacc gcattaccct tgcgctggca ggtttccgtc
    21541 aggaagattt agagattcaa ctggaaggta cgcgcctgag cgtaaaaggc acgccggagc
    21601 agccaaaaga agagaaaaaa tggctgcatc aagggcttat gaatcagcca tttagcctga
    21661 gctttacgct ggctgaaaat atggaagtct ctggcgcaac cttcgtaaac ggtttactgc
    21721 atattgattt aattcgtaat gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg
    21781 aacgtcccgc gttaaatagc taactagcta ttctctttgc cccgccattc tggtggggct
    21841 ttttttgtct tacttactca acaatgcgtt aaatttcgac tgtttaagat atttcggcac
    21901 gttctttccc gtgataatgt gcgctataag gcaaatctgc ttcaccgatt cagccataat
    21961 ccgtagcaat taaaaatgtt atctcaagag aaatggacga tgagtgatat agcattaacg
    22021 gtcagtattc tggctttggt ggcagtcgtc ggtttgttta tcggcaatgt caaatttcgc
    22081 ggcataggat taggtattgg cggcgtgctg tttggtggga tcatcgtcgg ccattttgtt
    22141 tctcaggcgg ggatgacatt aagtagcgat atgctgcatg ttattcagga atttggcctg
    22201 atcctgttcg tttatactat cgggattcag gtagggccgg gcttctttgc ctcattgcgc
    22261 gtctccggat tacgcctcaa cctgtttgct gttctgatcg tcatcatcgg tggtctggtt
    22321 accgccatcc tgcataaact gtttgatatt ccactgccgg tagtgctggg gattttctcc
    22381 ggtgcggtta ccaatacgcc agcgctgggg gcagggcagc agattttgcg cgacttgggt
    22441 acaccaatgg aaatggtcga tcagatgggg atgagttacg cgatggcgta tccattcggc
    22501 atttgcggga ttttgttcac catgtggatg ttgcgggtta ttttccgcgt caatgtcgag
    22561 acagaagctc agcagcacga gtcttcacgc accaatggcg gcgcgctgat caagactatc
    22621 aatattcgcg ttgagaaccc taacctgcat gatttagcca ttaaagatgt accgattctc
    22681 aacggcgaca aaattatctg ctcgcgtctg aaacgcgaag aaaccctaaa agttccttcg
    22741 ccagatacca ttatccaact gggcgatttg ctgcatctgg tggggcagcc agcggattta
    22801 cataatgcgc aactggtgat tggtcaggag gtcgatactt cgctgtccac gaaaggcact
    22861 gatttgcgcg tcgagcgtgt ggtggtcacc aatgaaaacg tgctcggaaa acgtattcgc
    22921 gacctgcact ttaaagaacg ctatgacgtt gttatctcgc gcctgaaccg tgccggggtc
    22981 gaactggtcg ccagtggcga tatcagcctg cagttcggcg atatcctcaa tctggtgggg
    23041 cgtccgtccg caattgatgc cgttgccaat gtgctgggga atgcgcagca aaaactgcaa
    23101 caggttcaga tgctgccagt gtttattggc atcgggcttg gcgtattgtt aggttctatt
    23161 cccgtctttg tgcctgggtt cccggccgcg ttgaaactgg ggctggcggg cggtccgctg
    23221 attatggcgt tgatcctcgg gcgtatcggc agtatcggca agctgtactg gtttatgccg
    23281 ccaagtgcca acctcgcgct gcgggagctg gggatcgtgc tgttcctctc ggtcgttggt
    23341 ctgaaatctg gtggagattt tgtgaatacc ctggtcaatg gcgaagggct aagctggatt
    23401 ggttatggtg ccctgatcac cgccgttccg ctgattactg ttggcattct ggcgcggatg
    23461 ttagccaaaa tgaattacct gaccatgtgc gggatgctgg caggttccat gaccgatcct
    23521 ccggcgctgg cgtttgctaa taatcttcat ccaaccagcg gtgcggcggc gctctcttac
    23581 gccactgtct atccgttagt gatgttcctg cgcattatca ccccccaatt actggcggtg
    23641 ctcttctgga gtatcggtta actcttttcc gggtggagtc gccgtaaatg gtactccacc
    23701 tggtattcgc tggcatttct gaacatcact gaataattca aaaactcccc gctctcgcta
    23761 tatgacaacg aggtgatccg cagcagtggc gtgtgttccg ctaccttcat ttgccgcgcc
    23821 agcagtctat cggcgagcac tggcgtcagg ctttcataat taccgccaat caaaatcccg
    23881 cactcttgtt caatatattc aaacttcgac ccttccagat gttgcagcga aagattacgg
    23941 aacagtttta ccggcatata gctgtcttcc agcatcagcg gtttcccttc cacaaaacga
    24001 acgcgacggg agaagtagat ctgctcgttg atttgaatcc gtaactggct ggcaatggcc
    24061 ggaggcgcag gcatgatttc aaaaatcagc acctggctgg tgaccgtttt tccctgccgt
    24121 tttaacacct ccaccagccc ggtcaggctg gcggtttgat gcagtacatc tttgcgcacc
    24181 aggtaagtgc cgctgccgtg gcggcggacc accaatcccc acgctaccag caggtcaatg
    24241 gctttacgga tggtcatccg cgataccgca aattcttccg ccaacttttt ttcaccggga
    24301 agaaggctat ttagcgtgaa atctgcggaa ttaagtcgaa ttcttaaccg ctccgcaatg
    24361 cttttgtaga tcatctctag atctcttttt tgcctgcaag cacgggtaat gcgcccacaa
    24421 gttctgctta atcgattgaa aaaacatctt ttttttaaag atgtgttcga tgctgtgtac
    24481 ctgtgctcac agatgtctac tttttcgcga aaacgtagat ctctaccgcc caacgaaaag
    24541 catgaaagcg atcacgaatc ccattgggtc gtcatgttct gccgatcgca tcttcctatc
    24601 ctcgctccag gcctgccgca taaccaatca ggcttcctac ttacagaatt gagaaaagag
    24661 gatgtggaaa tgctcagtca aattcaacgc tttggcggcg cgatgttcac gccagtgctg
    24721 ctgtttccct tcgccgggat tgtggtgggt cttgccatct tgctgcaaaa cccgatgttt
    24781 gtcggggaat cactgaccga tccgaacagt ttattcgcgc aaatcgtaca cattattgaa
    24841 gagggcggtt ggacggtgtt ccgtaatatg ccgctgattt ttgctgtcgg tttacccatt
    24901 ggccttgcta agcaagcgca ggggcgtgct tgtctggcgg tgatggtgag tttcctgacc
    24961 tggaactatt tcatcaacgc gatgggaatg acctggggaa gctacttcgg cgtcgatttc
    25021 actcaggacg cggtggcagg tagcggtctg acaatgatgg ccgggattaa aaccctcgat
    25081 accagcatta tcggcgcaat tatcatttcc ggcattgtga cggcgctgca taaccgtctg
    25141 ttcgataaaa aactgccggt ttttctcgac attttccagg ggacgtctta tgtggtgatt
    25201 atcgccttcc tggtgatgat cccctgtgcc tggctgacgt tgctcggctg gccaaaagta
    25261 caaatgggga ttgaatctct gcaagcgttc ctgcgttcgg cgggtgcgct tggggtgtgg
    25321 gtttacacct tcctcgaacg tattctgatc ccaaccggtt tacaccactt catctacgga
    25381 ccgtttatct ttggtccggc agctgttgaa ggcggcattc agatgtactg ggcgcagcat
    25441 ctgcaagagt tcagtctgag cgccgagccg ctgaaatcgt tgttcccgga aggcggtttt
    25501 gccctgcacg gtaactcaaa aatctttggt gccgtgggca tttctttagc gatgtacttc
    25561 actgccgcac cggaaaatcg ggtaaaagtg gcgggcttgc tgattcccgc aaccttaacc
    25621 gccatgctgg tgggaattac cgaaccgctg gaatttacct tcctgttcat ttcaccgttg
    25681 ctgtttgcgg tacacgccgt gctggcggcc tcaatgtcga ccgtgatgta tctctttggt
    25741 gtggtgggca acatgggcgg aggtctgatt gaccaggttt taccgcaaaa ctggatcccg
    25801 atgttcagca accacgcgga tatgatgctg acccaaatcg ccattgggtt gtgctttacc
    25861 ctgctgtact tcgtggtttt ccgcaccctg attctgcagt tcaacatgtg cacgccggga
    25921 cgtgaagatg cggaagtgaa actctactca aaagccgaat acaaagcctc gcgaggccaa
    25981 accaccgcgg cagagccaaa aaaagagctg gatcaggctg ccggtatcct gcaagccctg
    26041 ggcggggtcg gcaatatctc cagcattaac aattgcgcga cgcgtctacg cattgcactg
    26101 catgacatgt cacaaacgct ggatgacgaa gtctttaaaa agctgggagc gcacggcgtc
    26161 ttccgtagtg gcgatgccat tcaggtgatc attggtctgc atgtatccca attgcgtgaa
    26221 cagctcgata gcttaattaa ttctcatcaa tcagcagaaa atgttgccat tacggaggca
    26281 gtataatgac caaattctca gtggttgtcg caggcggtgg aagcaccttt acgccaggca
    26341 tcgtgttgat gctcctggcg aatcaggacc gtttcccgct tcgtgcactg aaattttatg
    26401 ataacgatgg cgcgcggcag gaagtgattg ccgaagcctg taaagtcatc cttaaagaaa
    26461 aagcgccgga cattgcgttt agttacacca ccgatcctga agtagcgttc agcgacgttg
    26521 attttgtcat ggcgcacatc cgcgtcggca agtacccgat gcgcgaactg gatgaaaaaa
    26581 tcccactgcg ccacggtgtt gttggtcagg aaacttgcgg acccggcgga atagcgtacg
    26641 gcatgcgttc cattggcggc gtcctggaac tggtggatta tatggaaaaa tattcaccaa
    26701 acgcctggat gctcaactac tccaacccgg cagcaattgt cgcagaagcc acgcgtcgtc
    26761 tgcgcccgaa tgcgaaaatc ctcaacatct gtgacatgcc aatcggtatt gaaagccgga
    26821 tggcgcaaat tgttgggctg caagatcgca aacagatgcg cgtgcgctac tacggcctga
    26881 accactggtg gtcggcgata tcccgcagtt tcagaaaggg ttaatgagtc agcaagtggc
    26941 ggtggaaaaa ctggtcgtgg acgcctggga acagcgttca tatcagcacc tgtggcaggc
    27001 gattacgttg tcgaaaacgg taccgagcgc ctcggtcgcc aaagctattc tggatgagtt
    27061 gctggaggcc aacaaagcgt actggccaga gttacgttaa ttgtgtggac tggcatccgt
    27121 tgggtgctgg tccgtattct ttagcacggc tgacggcgca taaccaaatt tatgtttaaa
    27181 ggctttgctg aaatgaaagg agtcgaagaa gttgagcgta tcagcgactt cgccaaccga
    27241 gtttccttgt tgctttaata gcgatagcgc cagatccaga cgcgcatcga ggtaatattc
    27301 tttcggcgtc ttgccggtat agcgtaaaaa cagacgacgc aaccaggctt cgctacaggg
    27361 gatcgtggca gccatatcag ctacgctcca gcgttgttgc agactggcat gtaaagtggc
    27421 aattaatttt tcaatttgcc gccgttgtgg atctttttta ccatctgcgt acataagaca
    27481 aatccattga taaataattt tcgtcagaaa agcgactgcc agattatttt ttattgcttc
    27541 tggtgaagtt attaactcag caacttcggt gagttcctga ttataaattt cgccattata
    27601 aataacgctt tgctgaccaa cgggaatatc catcatactg gtgggggtaa attccatcca
    27661 gtactgttcc cagactaaac cttcacagtg ataagagtga atatccattg gctttaagaa
    27721 tataatacag ttgccatgca gtgttatttg ttcgccattc tttaaaagaa ttttcccaca
    27781 tccttgtacg gtatacaccg caacccatga gttggcgata agcggtttct tcttgttccg
    27841 cggccagatc acccggtaat gctcgtcggc gtaggtatgc cagagcgaga tcagagaaat
    27901 tccgctggag atcacatttt cgttaatcaa taagggaata ttgtacggag tttcgttttt
    27961 tacatctgaa ctttgcaatt ttccattcat tttaatatcc ctccggcgaa ttatgctcac
    28021 aacctgaact taacagaaaa caatccgttc agggatacga cgtttgtatt ttaagaatct
    28081 gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg acataaacat
    28141 aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt ttacgctgct
    28201 ggtggcggtg atcacctggt ggaaggtccg caaaacagat accggatcgc aacaaggcta
    28261 ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa tgctaaccaa
    28321 cctttccacg gaacaactgg tcggcctttc cgggcaggcc tacaaaagcg gcatgtcggt
    28381 gatgggctgg gaagtgactt cagcggtgac gctgatcttc ctcgcgctaa tctttttacc
    28441 gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac gttatgataa
    28501 aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct gctttctgcc
    28561 gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg gggaatcgct
    28621 acagatttct cacggtgcgg ctatctggct actggtaatt ttgcttggtc tggcgggaat
    28681 tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca tcaacggtat
    28741 tgggctggtt atcggcgggt tgatggtgcc ggtatttggc ctgatcgcga tgggcaaggg
    28801 cagctttatg cagggcattg agcaactcac caccgttcac gccgagaaat taaactcaat
    28861 cggtggcccg accgatccct tgccgattgg cgcggcattt accggtttga ttctggtgaa
    28921 caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt caaaaagcct
    28981 ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg acccgctggt
    29041 actggtgctg ccagggttga ttgcgtttca tctgtatcag gatttaccga aagccgacat
    29101 ggcctacccg acgctggtca ataacgttct gccagtgcca atggtgggtt tcttcggcgc
    29161 ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg ccagtacgtt
    29221 attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc agcagctggt
    29281 caccgtcggg cgcaaatttg gtttctttat cgctatcgtt tcggtgctgg tcgcgccgtg
    29341 gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa cagctcaacg gcatttacaa
    29401 cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc cggcgctggc
    29461 ggcaaaagtg gcgatgggga ttggcataat cagctacatc accatcaact atctggtgaa
    29521 gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg tggtcgtgat
    29581 gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag atgcgtttgc
    29641 ggtggacatg aaaccgtgga gaaacgtcaa gatcgcgtca attggcatcc tgttcgcgat
    29701 gattggcgtc tatgccgggc tggctgaatt cggcggctac ggtacgcgct ggttagcgat
    29761 gatcagttat ttcattgccg ccgtagtgat tgtctacctg atttttgaca gctggcggca
    29821 tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag gatagcctat gaaacgcccc
    29881 aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg ctatagcggt
    29941 aaaccgctga atacgcaaaa tattgatagt ctggcggcgg aaggtattcg ctttaattcc
    30001 gcctacacct gttcaccggt ttgtacgcct gcacgcgccg gactatttac cggtatctac
    30061 gctaaccagt ccggcccgtg gaccaacaac gtcgcgccag gcaaaaacat ctccactatg
    30121 gggcgctact ttaaggatgc cggctatcac acctgttaca tcggcaaatg gcatctcgac
    30181 ggtcatgact atttcggcac tggcgagtgt ccgccggagt gggacgctga ttactggttc
    30241 gatggggcga actaccttag cgaactgacg gaaaaagaga tcagcctgtg gcgcaatggc
    30301 ctaaacagcg ttgaggattt acaggcgaac catatcgacg aaaccttcac ctgggcgcac
    30361 cgcatcagca atcgggcagt agattttctg caacagcccg cgcgcgccga ggaacccttc
    30421 ctgatggtgg tttcgtatga tgagccgcat cacccgttca cctgtccggt ggagtattta
    30481 gagaaatacg ctgattttta ctacgatctg ggcgagaaag ctcaggatga cctggcgaac
    30541 aaaccggaac atcaccgctt atgggcgcag gcgatgccat cgccagtcgg tgatgacggg
    30601 ctttatcacc atccgctcta ttttgcctgt aatgactttg ttgatgacca aatcggacgg
    30661 gtcatcaatg ccttaacgcc agagcaacgt gaaaatacgt gggtcattta tacttccgat
    30721 cacggcgaaa tgatgggcgc acataagctg atcagtaaag gagcggcgat gtatgacgac
    30781 atcacccgta ttccgctgat catccgttcg ccgcaagggg agcggcgaca ggtcgatacg
    30841 ccagtcagtc atatcgattt actgccgaca atgatggcgc tggcagatat tgaaaaacca
    30901 gagattctgc cgggggaaaa tatccttgcc gtgaaagagc cacgcggtgt aatggtggaa
    30961 tttaaccgct acgagattga gcatgacagc tttggcggtt ttattccggt gcgttgctgg
    31021 gtgacggatg actttaaact ggtactcaac ctcttcacca gtgatgaact ttacgatcgc
    31081 cgtaatgacc caaatgaaat gcataacctg atcgatgata tccgttttgc agacgttcgc
    31141 agcaaaatgc atgacgcctt attggattac atggacaaaa ttcgcgatcc gttccgcagt
    31201 taccaatgga gcctgcgtcc gtggcgtaaa gatgcactgc cgcgctggat gggggcattt
    31261 cgtccacgcc cacaagatgg ctattcgccg gtggtacgtg actatgacac cggcctaccg
    31321 acgcaaggag tgaaagtgga agagaaaaaa cagaagttct gacgataatg cccgctgatg
    31381 aacacccggc gggcattatt caggctgctt aaaccagtga accgccggtc aggaaggaaa
    31441 aaatcaggaa tatcagcaaa atgctgcaca cgatgccgat accaaaaagc aaggtatgga
    31501 aagccagcgt cccgccgcca aaaaccagcg cgccgcgaat gccccagcga ttttccttac
    31561 gcataccgcc aataaatgcg caaagtagcg cagcaatggc cagtgcgata ccgctttgat
    31621 cgaggatttt atcgacatct acgtcctctt ttttctccac ggtggtaatt ttttcgcctt
    31681 ttattccggc gagaagacct cttttaattt cagccgtttt gtccgccacg atgctttcaa
    31741 acgttggcgg tggtgcggaa aaagggccga atgaaaagtg gataatcccc atcattaaag
    31801 cgatggcacc gaacagcatg cccaatgaac tgattttatt ttgagctatg atacccatct
    31861 gacctccttg tagcggatgg cgaaagcata tcaggaaacg ggcggacgaa attcttaaaa
    31921 aaaaaccgct aaaggtagag cgttagtgaa attgcaaagg agcaacaatg aaaatttccc
    31981 gccttggaga agcgcccgat taccgcttct cgctggctaa tgagcgtact tttctggcgt
    32041 ggatccgtac cgcactagga tttctggcgg cgggggttgg gcttgatcaa cttgcgccag
    32101 atttcgccac gcccgtcatt cgtgaactgc tggcgttgtt gttgtgtctg ttttccggcg
    32161 ggttagcgat gtatggctac ctgcgctggt tacgcaatga aaaagcgatg cgtctgaaag
    32221 aggacctgcc ctacaccaac agcttattaa tcattagctt aatattgatg gtcgtggcgg
    32281 tgatcgttat gggactggtg ttgtatgccg gatagccgca aagccagacg cattgccgac
    32341 ccaggactgc agccggagcg cacatcactg gcgtggtttc gtaccatgct gggctacggc
    32401 gcgttgatgg cgttggctat caaacacaac tggcaccagg cgggcatgtt attctggatt
    32461 tcgattggca ttctcgccat cgtggcgctg atcctctggc actacacccg taatcgcaat
    32521 ttaatggatg tcacgaatag cgatttttcc caatttcacg tagtccgtga caaatttttg
    32581 atctccctcg cggtgttatc tctcgcaata ctgtttgctg taacgcatat acatcaactt
    32641 atcgtattta ttgagagagt cgcatgacag gaagtcaggt cattgatgct gaagaggaca
    32701 ggcataaact ggttgtagag tacaaggatg ctctgcaacc tgccgatttt tatcacaatt
    32761 tcaaacagcg gggcatccgc tccgtacagc tcattcccta tcttgaattt gatgaccggg
    32821 gcgatctgac ggccgcttcg gtgacagcag agctgtgggg gaaattttta attgccctgt
    32881 tcgagtgttg ggtgcgggca gatatcagtc gtatctcgat tgagcttttt gacgccacct
    32941 tacaaaaatg gtgtggcagc gaaaacccgc aaccgcgccg tgactgccag gcgtgcgact
    33001 ggcatcgcct gtgcccgcac gctcgccagg agacgccaga cagcgtgctt tgtgcgggct
    33061 atcaggcgtt ttattcttac tccgcaccgc atatgcgggt gatgcgtgat ctgataaagc
    33121 agcatcgttc tcccatggag ctgatgacga tgctgcggtg acgtgcgctt aaacgggctg
    33181 cccctgatgc gacatccgcg tcgccagcgg cagccagcac agcacgatca acaatcccat
    33241 taaggtcatc aacaacccca ggctgccctg accggtttgc ggcaacatcg cagagagcga
    33301 cgccagcacg ccggaaccaa tgttttgcag accgccgacc agcgcgccag ccgtgcccgc
    33361 caggaagggg aacggctcca tcgcgccgct ggtcgccagc ggaaacagca tcccggcacc
    33421 gaaaaagaac agcgcggcgg gaacgagcag cgtccagaca ttcatcacgc caaaccagtc
    33481 ggggatccac atcagcaagc cagccagcag gcagcagata accgactgcc acattaacgt
    33541 ggagaagcgt ttattgggac gtccggcaaa ccatgcgcca aaaaacgctg ccggaatcgg
    33601 cagaataaac aaaatactga ccgtcatact gctcagccct aacactgcgc ccatcagcac
    33661 gccggagcag gcttcaaagg cggcaatccc ggccagaccg ccaatcagca tcagcaaata
    33721 acagttaaaa ccgctgttac cgaaaagcgt tttataactg gtaagcaggc gcgtgcgcgg
    33781 tgcatcgacc ggacgcgttt ccggcatcca gcgggccata ctgaaggtca caccagcaca
    33841 aagaaccaac aaaaagagat aacaggcgcg ccagttccac atcgtatcca gcagaccgcc
    33901 gattagcggt gcgagcaacg gactgacgag aatccccatg tttaacaggc tgttagcatg
    33961 gcgcaactgt gtccgttcat ataaatctcg cggcaaagta cgcgccatta cgccgccaac
    34021 gccggtgccc atcccctgca tcgcgctggc ggcaatcaac accgtcaaac tggaggtcgt
    34081 gaccgcgacc agcgttgcca gcataaaaat ggacattccg acgaggatca ccggtcggcg
    34141 gcccacgcgg tcggaaatcg ggccataaaa cagctgtgag acaccgtaag tcagcagata
    34201 agcgcccatt acgctctgca ccgccccttc acggacgttg agatcgcgcg ccatatcggc
    34261 aatagctgga atataaatgg tttgcgccat ctgaccgacg gccacgagta ataccaacat
    34321 caataacaaa ttgacgtttc tttgcctttt cattatcacg gatgctttta taaaaataat
    34381 tagaataagt cactacgcat tcccataaat gcgggagagg aatctatcac attgatgaat
    34441 gttagccaga tatacgccag gaatggggaa ttgtttagcg gcagaatatg taaacaaaag
    34501 cggcaataaa tgttgccggg agggattacg tgcgaactgt aaccctctcc ccaaaggggc
    34561 gagggggaaa ccaccgttcc ctttcagaaa atgagctaaa cattgcacgg gacggtcccc
    34621 tcgccccttt ggggagaggg ctagggtgag gggcacaggc agcgcaggtg caaacattct
    34681 gctggtagaa gaagccccca actccaaccc gcacgctaaa tataaaaggg gagcggttac
    34741 ccgctcccct ttggtgcgac ttgaatctga attacttcag gtatttcaga acagcatcaa
    34801 gcagttgcag tgctgcaaca atgagtttga ggataagaat ggcgatatcc accaggctca
    34861 tacgcgtctc ctgtggttca ggagcactgc cagctgacgt acctttccgc tgcctgttgc
    34921 cagctgttgt gcacgcggta tcttaaggag aaacgttgtg cttgttgaca taacacagtg
    34981 tgctcacgcg gggttaaggc gctgacggca ccacccgttt cagccaggac ttagtgcgcc
    35041 gggtaacgat gcggcccccg cataacacat tgcgtacagt gatattatag aagtttactg
    35101 tataaataaa cagtaatatt tggacaaaac gcaaactgtg atcgatactc aactatgccc
    35161 taaatacgtc aaaattcgtg ccgaaattgc gcgttctgcg cggaacacgt atactttcag
    35221 tgttgacata atacagtgtg ctttgcggtt accagccgca ggcggctgat gaaacctcgc
    35281 tccggcgggg ttttttgtta cctgcaattc agtacaaaac gtgatcagcc cctcaatttt
    35341 cccttgctga aaaattttcc attgtctccc ctgtaaagct gtgcttgtat aaatattgtt
    35401 aaacacaaaa ccaacaaggt ccccaatgac tacttccatg ttcaacgcaa aactactacc
    35461 aactgcgcta tccgccgcag tggtcgtcgt gcgtgtggtg gtggtcgtcg gcaatgcgcc
    35521 gtagggactg gaacaacaca cgattccaaa accccgccgg cgcaaaccgg gcggggtttt
    35581 tcgtttaagc acctcccgga aagtcggccc agaagaaaag gactggagca tggcaagttc
    35641 gggcacaaca tcgacgcgta agcgctttac cggcgcagaa tttatcgttc atttcctgga
    35701 acagcagggc attaagattg tgacgggcat tccgggcggt tctatcctgc ctgtttacga
    35761 tgccttaagc caaagcacgc aaatccgcca tattctggcc cgtcatgaac agggcgcggg
    35821 atttatcgct caggggatgg cgcgcaccga cggtaaaccg gcggtctgta tggcctgtag
    35881 cggaccgggt gcgactaacc tggtgaccgc cattgccgat gcgcggctgg actccatccc
    35941 gctgatttgc atcaccggtc aggttcccgc ctcgatgatc ggcaccgacg ccttccagga
    36001 agtggacacc tacggcatct ctatccccat caccaaacac aactatctgg tcagacatat
    36061 cgaagaactc ccgcaggtca tgagcgatgc cttccgcatt gcgcaatcag gccgcccagg
    36121 cccggtgtgg atagacattc ctaaggatgt gcaaacggca gtttttgaga ttgaaacaca
    36181 gcccgctatg gcagaaaaag ccgccgcccc cgcctttagc gaagaaagca ttcgtgacgc
    36241 agcggcgatg attaacgctg ccaaacgccc ggtgctttat ctgggcggcg gtgtgatcaa
    36301 tgcgcccgca cgggtgcgtg aactggcgga gaaagcgcaa ctgcctacca ccatgacttt
    36361 aatggcgctg ggcatgttgc caaaagcgca tccgttgtcg ctgggtatgc tggggatgca
    36421 cggcgtgcgc agcaccaact atattttgca ggaggcggat ttgttgatag tgctcggtgc
    36481 gcgttttgat gaccgggcga ttggcaaaac cgagcagttc tgtccgaatg ccaaaattat
    36541 tcatgtcgat atcgatcgcg cagagctggg taaaatcaag cagccgcatg tggcgattca
    36601 ggcggatgtt gatgacgtgc tggcgcaatt gatcccgcag gtggaagcgc aaccgcgtgc
    36661 agagtggcac cagttggtag cggatttgca gcgcgaattc ccgtgtccaa tcccgaaagc
    36721 gtgcgatcca ttaagccatt acggcctgat caacgccgtt gccgcctgtg tcgatgacaa
    36781 tgcgattatc accaccgatg tgggtcagca tcagatgtgg accgcgcaag cttatccgct
    36841 caatcgccca cgccagtggc tgacctccgg tgggctgggc acgatgggtt ttggcctgcc
    36901 tgcggcgatt ggcgcggcgc tggcgaaccc ggatcgcaaa gtgttgtgtt tctccggcga
    36961 cggcagcctg atgatgaata ttcaggagat ggcgaccgcc agtgaaaatc agctggatgt
    37021 caaaatcatt ctgatgaaca acgaagcgct ggggctggtg catcagcaac agagtctgtt
    37081 ctacgagcaa ggcgtttttg ccgccaccta tccgggaaaa atcaacttta tgcagattgc
    37141 cgccggattc ggcctcgaaa cctgtgattt gaataacgaa gccgatccgc aggctgcatt
    37201 gcaggaaatc atcaatcgcc ctggcccggc gctgatccat gtgcgcattg atgccgaaga
    37261 aaaagtttac ccgatggtgc cgccaggtgc ggcgaatact gaaatggtgg gggaataagc
    37321 catgcaaaac acaactcatg acaacgtgat tctggagctc accgttcgca accatccggg
    37381 cgtaatgacc cacgtttgtg gcctttttgc ccgccgcgct tttaacgttg aaggcattct
    37441 ttgtctgccg attcaggaca gcgacaaaag ccatatctgg ctactggtca atgacgacca
    37501 gcgtctggag cagatgataa gccaaatcga taagctggaa gatgtggtga aagtgcagcg
    37561 taatcagtcc gatccgacta tgtttaacaa gattgcggtg ttttttcagt aaccgctcaa
    37621 ggcttgaaca acatcgcgct tatcgttaag gtaagcgcgt ttttttttac ccgccaggac
    37681 aagaccatga tcaccgttgc ccttatagac gatcacctca tcgtccgctc cggctttgcg
    37741 cagctgctgg ggctggaacc tgatttgcag gtagttgccg agtttggttc ggggcgcgag
    37801 gcgctggcgg ggctgccggg gcgcggtgtg caggtgtgta tttgcgatat ctccatgccc
    37861 gatatctccg gtctggaact gctaagccag ctgccgaaag gtatggcgac aataatgctc
    37921 tctgttcatg acagtccggc gctggttgag caggcgctta acgcgggggc acgcggcttt
    37981 ctctccaaac gctgtagccc tgacgaactg attgcggcgg tgcatacggt tgccacgggc
    38041 ggctgttatc tgacgccgga tattgccatt aaactggcat ccggtcgtca ggacccgcta
    38101 accaaacgcg aacggcaggt ggcggaaaaa ctggcgcaag gaatggcggt gaaagagatt
    38161 gctgccgaac tgggcttgtc gccgaaaacg gtgcacgtcc atcgcgccaa cctgatggaa
    38221 aaactgggtg tcagtaacga cgtagagctg gcgcgccgca tgtttgatgg ctggtgatga
    38281 agacgttgtt ctcccgctta attaccgtta ttgcctgctt ttttatcttc tctgccgcat
    38341 ggttttgcct gtggagtatc agcctgcacc tggttgagcg ccctgatatg gcggtgctgt
    38401 tatttccgtt tggtctgcgt ctggggctaa tgctgcaatg cccgcgcgga tactggccgg
    38461 tgctgctggg cgcggagtgg ctgctgattt actggctaac gcaggcggtc ggtttaaccc
    38521 attttccgtt attgatgatc ggtagtttac tgacgttact gcccgtggcg ctgatctcgc
    38581 gctatcgcca tcagcgtgac tggcgcacct tgctgttaca gggtgcggcg ttaacggcgg
    38641 cggcgttgtt gcagtcgctg ccctggcttt ggcacggcaa agagtcgtgg aatgcgctgt
    38701 tgctgacttt aactggcggc ctgacgctgg ccccgatatg tctggtgttc tggcactatc
    38761 tcgccaataa cacctggctg ccgctcggcc cgtcactggt ttctcagccg atcaactggc
    38821 gcgggcgaca tctggtctgg tacttgctgc tgtttgttat cagtctctgg ctccagttgg
    38881 gattgccgga cgaactgtcg cgctttacgc cattttgtct ggcgctgccg attatcgcgc
    38941 tggcctggca ctatggttgg caaggggcgc tgattgcgac gttgatgaac gccatcgcgc
    39001 tgatcgccag tcaaacctgg cgcgatcatc cggtggattt attgctctcg ctgctggtgc
    39061 aaagtctgac agggttgttg cttggcgctg gcatccagcg gttgcgtgaa cttaaccagt
    39121 cgctgcaaaa ggaactggcg cgcaatcagc atctggctga acggttgctg gaaaccgaag
    39181 agagcgtgcg ccgtgatgtg gcgcgtgagc tgcatgatga tatcggtcag accatcacag
    39241 ccattcgtac tcaggcgggc attgttcagc ggctggcggc agataacgcc agcgtgaagc
    39301 agagcgggca gctcatcgaa caactatcgc tgggcgttta cgacgcggtg cgccgtttgt
    39361 tgggtcggtt acgtccgcgc cagttggatg atctcaccct ggagcaggcc atccgctcac
    39421 tgatgcggga aatggagctg gaagggcgcg gtattgtcag ccatctcgaa tggcgaatcg
    39481 atgaatcagc gttaagcgaa aaccagcgcg tgacgctgtt tcgtgtctgc caggaagggc
    39541 tgaacaacat tgtgaaacat gctgatgcca gcgcggtcac cctgcaaggc tggcagcagg
    39601 atgaacggtt gatgctggtt attgaagacg atggcagcgg tttgccgccg ggttccgggc
    39661 aacaaggttt tggcctcacc ggaatgcgcg agcgcgtaac ggcgctgggt ggcacattac
    39721 acatttcctg tctgcacggc acgcgtgtca gcgtttctct acctcaacgc tatgtctaag
    39781 gtttgatgat gttgccgttt ctgaaagcgc ctgccgatgc gccattaatg actgataaat
    39841 atgaaattga tgcccgctat cgctactggc gtcggcatat tctgctgacc atctggctgg
    39901 gttacgcgct gttttacttc acgcggaaaa gttttaacgc cgcggtacca gaaatccttg
    39961 ctaacggcgt gctcagccgt agcgatatcg gcctgttagc gaccctgttt tacattactt
    40021 acggcgtgtc gaagtttgtc tccggcattg tcagcgatcg ctcaaatgcc cgttatttta
    40081 tggggatagg gcttatcgcc acgggcatca tgaacattct gtttggcttc tcgacgtcgc
    40141 tatgggcgtt tgccgtgctc tgggtgctga acgccttttt ccagggctgg ggttcaccgg
    40201 tgtgtgcgcg tctgttaacg gcctggtatt cacgtaccga gcgcggcggt tggtgggcat
    40261 tatggaacac ggcgcataac gtcggcggcg cactcattcc cattgtgatg gcagcgagcg
    40321 cgctgcatta cggctggcgt gccgggatga tgattgctgg ttgtatggcg atagtcgtgg
    40381 ggatttttct ctgctggcgg ctacgcgatc gcccgcaggc gttaggttta ccggcggtcg
    40441 gtgaatggcg gcacgacgcg ctggaaattg ctcaacaaca agaaggggca gggttgacgc
    40501 gtaaagagat cctgaccaaa tatgtgttgc tgaatccgta tatctggctg ctttcgtttt
    40561 gctatgtgct ggtctatgtg gtccgggcgg caatcaacga ctggggcaat ttgtatatgt
    40621 ccgagacgct gggcgtcgat ctggtcacgg cgaatacggc agtgacgatg tttgaactgg
    40681 gcggatttat cggtgcgctg gtagccggtt ggggctcgga caaattgttt aacggcaacc
    40741 gtgggccgat gaatttgatt ttcgccgccg gaattttgct ttcagtcggc tcgctgtggc
    40801 tgatgccatt tgccagctac gtgatgcagg caacctgctt cttcaccatt ggtttttttg
    40861 tctttggccc gcagatgtta atcggcatgg cggcggcaga gtgttcccac aaagaggctg
    40921 caggggcggc gacggggttt gtcggcttgt ttgcttatct gggggcgtcg cttgctggtt
    40981 ggccgctggc gaaagtactc gatacctggc actggagcgg attttttgtg gttatctcta
    41041 tcgccgccgg ggtttccgca ctgctgttac tgcccttttt gaacgcccag gcaccgcgcg
    41101 aagcgtgatg catctcacct tttcacttca tatccggcaa aactaagaaa ttttctaggt
    41161 tttgcctgga cgctatctca ggcctgattt gctgctgatt tttacaatgc catcattcat
    41221 taattctttc ccaaaataat tcaagttgca ggaacgcagc aaacgcgtga gtcccgggag
    41281 catagtgaac tatgtgaccg ggaggaatgc gtgcagctaa ggcacctgcg gcttgaagta
    41341 tgaagggaaa tcaggagtaa cccatgctgg ctttcttaaa ccaggttcgc aagccgaccc
    41401 tggaccttcc gctcgaagtg cggcgcaaaa tgtggttcaa accgttcatg caatcctacc
    41461 tggtggtctt tatcggctac ctgacgatgt acctgattcg caagaacttt aacatcgcgc
    41521 agaacgacat gatctcgacc tacgggttga gcatgacgca gctggggatg atcggcctgg
    41581 gtttctccat cacttatggc gtgggtaaaa cgctggtttc ctactacgcc gacggcaaaa
    41641 acaccaaaca attcctgccg ttcatgctga tcctgtctgc catttgtatg ctgggcttca
    41701 gtgccagtat gggcagcggc tcggttagcc tgttcctgat gattgccttc tacgccttaa
    41761 gcggcttttt ccagagtacc ggcggttcgt gcagttactc caccatcacc aaatggacgc
    41821 cgcgtcgtaa acgcgggaca ttcctcggtt tctggaatat ttctcacaac cttggtggtg
    41881 caggcgcagc aggtgtggcg ctgttcgggg caaattacct gttcgatggc catgtcatcg
    41941 gcatgtttat cttcccgtcg attatcgcgc tgattgtcgg ttttatcggc ctgcgttacg
    42001 gcagcgactc cccggaatct tatggcctcg gcaaagctga agaactgttc ggcgaggaga
    42061 tcagcgaaga ggacaaagag acagaatcta ccgatatgac caagtggcag atctttgttg
    42121 agtatgtgct gaaaaacaaa gtgatctggc tgctgtgctt cgccaacatt ttcctctatg
    42181 tggtacgtat tggtatcgac cagtggtcaa ccgtatacgc gttccaggaa ctgaaactct
    42241 ctaaagcggt ggcgattcag ggctttacgc tgtttgaagc tggtgcactg gtcggtacgc
    42301 tgctgtgggg ctggctctct gacctggcga acggtcgccg tggcctggtg gcctgcatcg
    42361 cgctggcgct gattatcgcc acgctcggtg tgtatcaaca tgccagcaac gaatatatct
    42421 atctggcttc tctctttgcg ttgggtttcc tggtctttgg cccgcaattg ttgattggtg
    42481 tggctgctgt tggctttgta cctaaaaaag cgattggcgc tgccgatggt attaaaggca
    42541 cctttgctta cctgattggt gacagctttg ccaagttagg tctgggaatg attgccgatg
    42601 ggacgccggt attcggcctt accggctggg caggcacctt cgccgcactt gatatcgccg
    42661 cgattggttg tatctgcctg atggcgatag tggcggtgat ggaagaacgc aaaatccgcc
    42721 gcgagaaaaa aattcagcag ttgacagtgg cataaacgta actggtgact tttgcccggc
    42781 atgacgccgg gcttttttat tattccgtga cttccagcgt agtgaaggca aacttctcgc
    42841 catcaaatag cccctgactg gttagtttta gcgcggggat cactggcaga gaaagaaacg
    42901 ccatctgaat aaacggctca tcgggtaacg gaccgcattc acgggcggcg gctttcaagg
    42961 cgtcaatttg ttccgccagc gactgcgccg tgtcggtgct catcaggccg gcaatgggca
    43021 acggcagatg actttggacc tggccgttac gtaccacgca cagcccgccg ccatcctgaa
    43081 tcacctgatt gaccgccagc gccatctctt cggcactgcg accgatcacc acaatattat
    43141 ggctgtcatg gctgaccgtc gcagccagcg caccttcatt cagtccaaag ccgccaagca
    43201 aaccacaagc cggagccagc cgttgcccgt aacgctcaag tacggcaata aagctcacat
    43261 catcgcgatc aaaaccattt tcgctgtaga cgctggagtg ggagtgcgta atcaattcgt
    43321 tatggatgac gtcaatgacc cgatagcgtt ttccgggcgt aaattgcagg gcaaagtcgc
    43381 tggcggaaac tggctggcgg gcaatggtgt tgccatatgg cggagcggat tgtgccagtc
    43441 tcgccgactc ttccgcctgt aaggtttgcg catcaatcgg ctcgcctttc accagtacct
    43501 gctgcaccgt gaccttacgc gcatcgctca acaggacgat atcggcctgc ttgccgggtg
    43561 ccagtaagcc gaggtgattc agaccaaagt ggcgcgccgt cgaccagctg gcgacgcgat
    43621 atgccacatg cagcggcaca ttgtgttgtt cgatcaggcg gcgaattaag gcatcgatgt
    43681 gtccttcatg ggcgatctcc cacgggttac ggtcatcggt acagagcatg cattgcgggc
    43741 tgttaaattc gttgatcaac ggtgccagcg cgttgagatt gcgggcagcg gacccttcgc
    43801 ggatcatcaa cgacatgccg agttgtaatt tccggcgtcc ttcttccagc tgataacttt
    43861 cgtggcagtt ttcaataccc gcagtaatat aggcgttaag ttctttacca cccaaacccg
    43921 ggcagtgacc gtccagcgtc aggtggcgaa atgcatccag tttatcgagc agcgcattct
    43981 gcccgctaat tacgccaggg tagtccatca tttctgcaag gccggtaacc tgcggatggt
    44041 cccgccaggc gagcatcaat ccgctgcaaa gccggagcat cagcgtattc tgcgccaaca
    44101 ccggcaatgt aacgtccttt aatcacaatt gggccggaaa tttctccgcc attgatcagg
    44161 tcgagaatag agacattatc aataatataa tcggcaacag cgtcgccacg ggaaacggct
    44221 aacaattcct ggtattcagc ccggctaatg t
//
LOCUS       DAZUFB010000029        44134 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZUFB010000029 DAZUFB010000000
VERSION     DAZUFB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.029"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..1215
                     /locus_tag="ACHY3M_001848"
     CDS             127..1215
                     /locus_tag="ACHY3M_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFS4316777.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLVVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1303..2601)
                     /gene="exuT"
                     /locus_tag="ACHY3M_001849"
     CDS             complement(1303..2601)
                     /gene="exuT"
                     /locus_tag="ACHY3M_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFS4316778.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            3084..4496
                     /gene="uxaC"
                     /locus_tag="ACHY3M_001850"
     CDS             3084..4496
                     /gene="uxaC"
                     /locus_tag="ACHY3M_001850"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4316779.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            4511..5998
                     /gene="uxaA"
                     /locus_tag="ACHY3M_001851"
     CDS             4511..5998
                     /gene="uxaA"
                     /locus_tag="ACHY3M_001851"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFS4316780.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            6081..6632
                     /gene="ygjV"
                     /locus_tag="ACHY3M_001852"
     CDS             6081..6632
                     /gene="ygjV"
                     /locus_tag="ACHY3M_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFS4316781.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(6637..7881)
                     /gene="sstT"
                     /locus_tag="ACHY3M_001853"
     CDS             complement(6637..7881)
                     /gene="sstT"
                     /locus_tag="ACHY3M_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4316782.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(8280..9255)
                     /gene="alx"
                     /locus_tag="ACHY3M_001854"
                     /pseudo
     CDS             complement(8280..9255)
                     /gene="alx"
                     /locus_tag="ACHY3M_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
     gene            complement(9538..10524)
                     /gene="ygjR"
                     /locus_tag="ACHY3M_001855"
     CDS             complement(9538..10524)
                     /gene="ygjR"
                     /locus_tag="ACHY3M_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4316783.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(10603..11286)
                     /gene="ygjQ"
                     /locus_tag="ACHY3M_001856"
     CDS             complement(10603..11286)
                     /gene="ygjQ"
                     /locus_tag="ACHY3M_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFS4316784.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(11363..11866)
                     /gene="ygjP"
                     /locus_tag="ACHY3M_001857"
     CDS             complement(11363..11866)
                     /gene="ygjP"
                     /locus_tag="ACHY3M_001857"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS4316785.1"
                     /translation="MSNLTYLQGYPELLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11951..13087
                     /gene="rlmG"
                     /locus_tag="ACHY3M_001858"
     CDS             11951..13087
                     /gene="rlmG"
                     /locus_tag="ACHY3M_001858"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFS4316786.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13278..13361
                     /locus_tag="ACHY3M_001859"
                     /pseudo
     CDS             13278..13361
                     /locus_tag="ACHY3M_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            13358..13774
                     /gene="higA"
                     /locus_tag="ACHY3M_001860"
     CDS             13358..13774
                     /gene="higA"
                     /locus_tag="ACHY3M_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFS4316787.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13819..15837)
                     /gene="fadH"
                     /locus_tag="ACHY3M_001861"
     CDS             complement(13819..15837)
                     /gene="fadH"
                     /locus_tag="ACHY3M_001861"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFS4316788.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAIELDARRAIAQGTRLALEI"
     gene            complement(16163..18514)
                     /gene="ygjK"
                     /locus_tag="ACHY3M_001862"
     CDS             complement(16163..18514)
                     /gene="ygjK"
                     /locus_tag="ACHY3M_001862"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4316789.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(18531..19601)
                     /gene="ygjJ"
                     /locus_tag="ACHY3M_001863"
     CDS             complement(18531..19601)
                     /gene="ygjJ"
                     /locus_tag="ACHY3M_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFS4316790.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(19735..21168)
                     /gene="ygjI"
                     /locus_tag="ACHY3M_001864"
     CDS             complement(19735..21168)
                     /gene="ygjI"
                     /locus_tag="ACHY3M_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4316791.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTILSMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21231..21680)
                     /gene="ebgC"
                     /locus_tag="ACHY3M_001865"
     CDS             complement(21231..21680)
                     /gene="ebgC"
                     /locus_tag="ACHY3M_001865"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFS4316792.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21677..24769)
                     /gene="ebgA"
                     /locus_tag="ACHY3M_001866"
     CDS             complement(21677..24769)
                     /gene="ebgA"
                     /locus_tag="ACHY3M_001866"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFS4316793.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQDNEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYELGAGFFSTNLHSENKQ
                     "
     gene            complement(24953..25936)
                     /gene="ebgR"
                     /locus_tag="ACHY3M_001867"
     CDS             complement(24953..25936)
                     /gene="ebgR"
                     /locus_tag="ACHY3M_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFS4316794.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26155..26487
                     /gene="ygjH"
                     /locus_tag="ACHY3M_001868"
     CDS             26155..26487
                     /gene="ygjH"
                     /locus_tag="ACHY3M_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFS4316795.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(26529..27908)
                     /gene="ygjG"
                     /locus_tag="ACHY3M_001869"
     CDS             complement(26529..27908)
                     /gene="ygjG"
                     /locus_tag="ACHY3M_001869"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFS4316796.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28326..29846
                     /gene="aer"
                     /locus_tag="ACHY3M_001870"
     CDS             28326..29846
                     /gene="aer"
                     /locus_tag="ACHY3M_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFS4316797.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VCEGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(30000..30623)
                     /gene="nfeR"
                     /locus_tag="ACHY3M_001871"
                     /gene_synonym="yqjI"
     CDS             complement(30000..30623)
                     /gene="nfeR"
                     /locus_tag="ACHY3M_001871"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFS4316798.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30900..31664
                     /gene="nfeF"
                     /locus_tag="ACHY3M_001872"
                     /gene_synonym="yqjH"
     CDS             30900..31664
                     /gene="nfeF"
                     /locus_tag="ACHY3M_001872"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFS4316799.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVSSGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(31718..31793)
                     /locus_tag="ACHY3M_001873"
     tRNA            complement(31718..31793)
                     /locus_tag="ACHY3M_001873"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31758..31760),aa:Ile,seq:cat)
     gene            31918..32424
                     /gene="mug"
                     /locus_tag="ACHY3M_001874"
     CDS             31918..32424
                     /gene="mug"
                     /locus_tag="ACHY3M_001874"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFS4316800.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(32503..34344)
                     /gene="rpoD"
                     /locus_tag="ACHY3M_001875"
     CDS             complement(32503..34344)
                     /gene="rpoD"
                     /locus_tag="ACHY3M_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4316801.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(34539..36284)
                     /gene="dnaG"
                     /locus_tag="ACHY3M_001876"
     CDS             complement(34539..36284)
                     /gene="dnaG"
                     /locus_tag="ACHY3M_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4316802.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            complement(36395..36610)
                     /gene="rpsU"
                     /locus_tag="ACHY3M_001877"
     CDS             complement(36395..36610)
                     /gene="rpsU"
                     /locus_tag="ACHY3M_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4316803.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            36848..37861
                     /gene="tsaD"
                     /locus_tag="ACHY3M_001878"
     CDS             36848..37861
                     /gene="tsaD"
                     /locus_tag="ACHY3M_001878"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4316804.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(37904..39367)
                     /gene="ttdT"
                     /locus_tag="ACHY3M_001879"
     CDS             complement(37904..39367)
                     /gene="ttdT"
                     /locus_tag="ACHY3M_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFS4316805.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(39415..40020)
                     /gene="ttdB"
                     /locus_tag="ACHY3M_001880"
     CDS             complement(39415..40020)
                     /gene="ttdB"
                     /locus_tag="ACHY3M_001880"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFS4316806.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(40017..40928)
                     /gene="ttdA"
                     /locus_tag="ACHY3M_001881"
     CDS             complement(40017..40928)
                     /gene="ttdA"
                     /locus_tag="ACHY3M_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFS4316807.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            41135..42067
                     /gene="ttdR"
                     /locus_tag="ACHY3M_001882"
                     /gene_synonym="dan"
     CDS             41135..42067
                     /gene="ttdR"
                     /locus_tag="ACHY3M_001882"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFS4316808.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDKGYQVM"
     gene            complement(42080..42697)
                     /gene="plsY"
                     /locus_tag="ACHY3M_001883"
     CDS             complement(42080..42697)
                     /gene="plsY"
                     /locus_tag="ACHY3M_001883"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4316809.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            42802..43170
                     /gene="folB"
                     /locus_tag="ACHY3M_001884"
     CDS             42802..43170
                     /gene="folB"
                     /locus_tag="ACHY3M_001884"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFS4316810.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            43260..44081
                     /gene="bacA"
                     /locus_tag="ACHY3M_001885"
     CDS             43260..44081
                     /gene="bacA"
                     /locus_tag="ACHY3M_001885"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4316811.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
BASE COUNT        10532 a        11435 c        11756 g        10411 t
ORIGIN      
        1 cagcggagat gttacttgta gtgtggtgac ttattcacaa ttaccggagt cgattcaggt
       61 tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc aaagggcggt
      121 tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac gctcactggc
      181 gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg
      241 gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa
      301 tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa
      361 atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa
      421 acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa agggcttcag
      481 acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata
      541 tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt
      601 ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca ggactatttc
      661 gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc
      721 gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc
      781 aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat
      841 gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg gcgcagagac
      901 ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga acgcattgat
      961 taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa
     1021 aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact gcccggtatt
     1081 cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg tagtcgataa attcagcgtg
     1141 atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg
     1201 ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa acgacgaagc
     1261 cgtctgataa agcggcttcg tcattacaac ttaagttaaa cgttaatgtt gcggtgcggg
     1321 atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca cggtccagat
     1381 aaccagcgca cctaacaggt cgaacactgc cagaactgcg aacagcgggc tgaagccgat
     1441 ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat
     1501 cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag aagagagcgt
     1561 aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc
     1621 gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca cagcacccag
     1681 cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg
     1741 cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa
     1801 catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc
     1861 gttaaaagta ccccaggccg gttctgccag gaaacgcggc agcgcgatac cccagaactg
     1921 acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc
     1981 ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg
     2041 atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat
     2101 aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg gcgcaatcat
     2161 cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct ctttcgccgg
     2221 gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac
     2281 tgcaccacga gcaatcgcca ggccacccca gctaccagcc agcgcggttg caccacagaa
     2341 cacggcccac agcacagcaa acattgcata accgattttc gtacccagca catccagtac
     2401 ataacctgct accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga
     2461 atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt
     2521 gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg
     2581 taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg
     2641 gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg
     2701 gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc
     2761 cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga
     2821 cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca aatgtgcgaa
     2881 tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagagaga
     2941 tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc
     3001 ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct
     3061 cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata
     3121 ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc
     3181 attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca
     3241 tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc
     3301 gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc
     3361 cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgtttg
     3421 gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg
     3481 aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa
     3541 tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag
     3601 acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg
     3661 aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc
     3721 gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg
     3781 gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg
     3841 aactggacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg
     3901 cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg
     3961 tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg
     4021 ggccggatgt aggctttgac tccatcaacg accgtccgat ggcagaagag ttgtctaagc
     4081 tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac tgcctgaacc
     4141 cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg
     4201 gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc
     4261 agatgaccca actggcgcag ctcggtttgc tgagccgctt tgtcggtatg ctgactgaca
     4321 gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga
     4381 tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacttgctg ggcgagatgg
     4441 tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc
     4501 tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc
     4561 ggatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga
     4621 tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca atgtcatcaa
     4681 atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc
     4741 ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ctgattttca
     4801 ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg ctaacggcga
     4861 tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc
     4921 gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt
     4981 gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca tcaacacccg
     5041 caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct
     5101 gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga
     5161 acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca
     5221 tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga
     5281 actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc
     5341 gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga
     5401 agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac
     5461 gtttgaaaaa ctggtgacca tggtcaacga cttcaaacag tactttattg cccatgacca
     5521 gccgatctat gaaaacccat cgccggggaa caaagcgggc ggtatcacca cgctggaaga
     5581 taaatccctt ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg tgctgcgtta
     5641 cggcgagcgt ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta acgatgccgt
     5701 agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgtgg
     5761 cacgccatac ggcggctttg taccgacggt gaaaatcgct accaacagtg aactggcggc
     5821 gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc
     5881 gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta aacaaacctg
     5941 caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag
     6001 tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc
     6061 atacctgata aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg
     6121 cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc
     6181 tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg
     6241 gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat
     6301 gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc
     6361 ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta
     6421 aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact
     6481 tctgggcggg gtcgataggc ggcacaatga ttgagggtag ttttctgctt atgaatggct
     6541 tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga
     6601 aaacccctcc cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc
     6661 cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt
     6721 cagcgcggtt tcgcaagagt cctgcaatac gccgatgata aagccgacgg caaccacctg
     6781 catggcgata tcgttcgaaa taccgaacat attacaggcc agcgggatca gcagcagaga
     6841 accccccgcc acgccggatg cgccacaggc acacagagaa gccaccacgc tcaacagcag
     6901 cgccgtgggc agatcgaccg ggatacccag cgtattaacc gcagccagcg tcaacacggt
     6961 aatggtgatt gctgcgcccg ccatattgat ggtggcaccc agcggaatag aaacggaata
     7021 ggtatcgcga tccagattca gcttttcaca cagtgccata ttcaccggaa tgttcgccgc
     7081 agagctgcgg gtgaagaagg catacacgcc gctttcgcgc aggcacagca gcaccagcgg
     7141 gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag
     7201 taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc
     7261 ggtggttgcc agggtagaag aaaccagacc aaaaatacca atcggtgcga agcgaatgac
     7321 cagtttcacc ataaaggtga cggcattcga catatcgttg accaggtttt tggtggtctc
     7381 gttaccgtga cgcagtgcga aaccgaggcc aattgcccac accaggatcc cgatgtagtt
     7441 acctttcagc agcgcgtcga tggggttgga aaccatgctc attaccagcc cgcgcatcac
     7501 ttctacaatg cctgacggtg gcgaaatatc acccgcgcta ctggataagt gcagggtaga
     7561 agggaaggcg aagctgaaaa cgactgcggc cagagcagca gagaaggtac ccagcagata
     7621 gaggaacaaa atagggcgga tattggtttt ctgcccgtgc tggtggttag caatagatgc
     7681 catcaccagc atcaacacca ggatgggggc aacggctttc agtgcgccga cgaacaaagt
     7741 acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag
     7801 aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc
     7861 cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg
     7921 cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa
     7981 agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcata ttttgcaact
     8041 taatggttac atttatttga cataaaaata aagccaacgt gcggcttcca gactgatgac
     8101 aacatcaaaa aaggactcgg tcagtcccct cgccccgttt ggggagaggg ttagggtgag
     8161 gggaacaggc cagcatcggt gcgaaaaatt accctcaccc cgaccctctc cctggaaggg
     8221 agagggagaa aacggtgctc atgttaatga cagggtacac ggcttaggca gattaaaaat
     8281 tatccacccc gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc
     8341 accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca
     8401 atcagcatct tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag
     8461 aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gattgcaaac
     8521 aggtttgagg tcagcacaat aaacggatca gtggtcacgg cgaagatagc cggaatgcta
     8581 tccacggcaa aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg
     8641 gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg
     8701 cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca
     8761 tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc
     8821 cagtcgaact gtgaaatcag ccagctgcct gtgaagatca tgatggtacg cagaacaatc
     8881 gccccgagca cgccatacac cagcacgcgg cgttgtaatg ccgccggaac agagaaatag
     8941 ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagatag
     9001 cccgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcagt ttgcaccaga
     9061 taccaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
     9121 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
     9181 caggtcgata gccagcataa tggcgacaac aacagcgaat ccgccccata gcaacggcgt
     9241 gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc
     9301 agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta caacaaaaaa
     9361 ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt tttcacacat
     9421 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
     9481 accattcagc cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaatta
     9541 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
     9601 cttggcggtg atgcgactga ccgccagccc cggatgatcc accagatgct catccaccag
     9661 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
     9721 ctgcatttgg ctgccacgcg gcacgaagca cactttctgg cattcagaca gtttttcaat
     9781 caccagcgat cctgcttcgc cctgaatctc gctcgccagg acagaatcac tgacttttga
     9841 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcatctac
     9901 accgcttgcc agcaaactgg cggttgcctg cacgcttttc ggctcaccaa ataacgccac
     9961 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgacggatt
    10021 aaaggtgttg ggattttcac cgtccaggta acgttgatag cgcgaggaat actggcaata
    10081 gttgaaaaag actttacgca atttgccgac tttcggcagc gcctggcgca gcaaatgaaa
    10141 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cccgcgcaca
    10201 ggcaatggcg gcatccactt ccgccagatt cgacgccagt ggtttctcgc aaatcacatg
    10261 aattttatgg ctaaggaaaa gttgtgtctg ggaaaagtgc agggcattcg ggctggcaat
    10321 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa acagatgctc
    10381 gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat atacggcggt
    10441 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
    10501 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgtcgtca
    10561 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt ttgccggaca
    10621 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctggcgatg
    10681 gaggattttg gcgtcaatca cggcgctgac acgagcgagt ttctcccgca gttgagtata
    10741 aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg atagcgtcga tgccgtactg
    10801 ttttgccagc cagatggcgc gttgattatg aaattcttgc gagataatgg tgatatggtt
    10861 ttcaccaaaa acctttttgg ctcgcactac cgaatccagc gttgagaatc cggcatagtc
    10921 gcagaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca tacctgaggc
    10981 ttcatcgtaa tttttacgtc cgttatcgcc acttaccagt agccatttca cttttccggc
    11041 gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac ccggcctggc
    11101 ccccagcaat aagcccacgt tacgcgccgg aactgcgttg acatcgctcc aggtcagctg
    11161 cttgctggcg ttgaccataa cgcgatcggc aataaaaatg gtcgctcctg ctataagcaa
    11221 caacaaacag ccaatcttaa gagtccggcg agaaaaacaa atacggagaa gaaggcgggg
    11281 ctgcatggtc gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaagaa
    11341 aagataacgc ctgacatcat tttcagattt tgtcctgccc aagcgacaac tgcgttagcc
    11401 acagccgggt gtcgaactca agctggtggt actgcggctc catatggcag cacaactggt
    11461 aaaacgcttt gttatgctct ttctccttca ggtgcgccag ttcgtgcacc acgatcatac
    11521 gtaaaaacgg ttccggcgcg ttgcgaaaca cagtagcgac gcggatctct gcttttgctt
    11581 tcagcttgcc gccctgcaca cgagaaacag cggtatgtaa cccgagcgcg ttttttagca
    11641 catggatctt attgtcatac atcactttat tgatcggcgg ggcattgcgc agaaactgat
    11701 tttttagatc cggagtatat tgccagaggg ctttatcggt ggcgtagtcg tgtgttcccg
    11761 gatagcgttt tgccagcaca tcgcccagac gctgttcgtt aatcagcgtt cgcacctggg
    11821 aaagtagcag ctcgggataa ccctgaagat aagtaagatt gctcataacc gcccacgaaa
    11881 aagagaaaaa gggtatactc acgcaccctt ttcaggggca acgtcgaaat tttatcattc
    11941 aggagggccg atgagccact tagacaacgg tttccgttca ctgacactac aacgttttcc
    12001 ggcgacggat gacgttaacc cgctacaggc gtgggaagcg gcggatgaat atttgctgca
    12061 acagttggac gacacagaaa tccgcggccc ggtgttgatc ctgaatgatg cctttggtgc
    12121 gttaagctgc gcgctggcgg aacataagcc gtacagcatt ggcgactcat acatcagtga
    12181 actggcgacg cgcgagaatt tacgcctcaa cgggattgat gagtcgagcg tgaagtttct
    12241 cgacagcacc gccgactacc cgcaacagcc gggtgtggtg ctgatcaaag tgccgaaaac
    12301 actggcattg ctggaacaac aactgcgtgc gctgcgcaaa gtggtcacgt cggatacacg
    12361 tattattgcc ggtgccaaag cccgtgacat tcacacttcc acgctggaac tgttcgaaaa
    12421 agtgctcggc ccgaccacca ccacgctggc atggaaaaaa gcgcgcctga ttaattgcac
    12481 gttcaatgaa ccgccgctgg ccgatgcgcc gcagaccgtt agctggaagc tggaaggtac
    12541 tgactggact atccataacc atgcgaatgt cttctcccgc accgggcttg atatcggcgc
    12601 gcgcttcttt atgcaacatc tgccagagaa tctcgaaggg gagattgtcg atctcggttg
    12661 cggtaatggc gttattggtc tgacgctgct tgataaaaac ccgcaggcga aagtggtgtt
    12721 tgtcgatgaa tcgccgatgg cggttgcttc cagccgtttg aacgttgaaa ccaacatgcc
    12781 agaggcgttg gatcgcagcg agtttatgat caacaacgcg ctctccggcg tggagccttt
    12841 ccgctttaat gctgtgctct gcaacccgcc gtttcaccaa caacatgcgc tgaccgataa
    12901 cgtcgcctgg gagatgttcc accatgcccg ccgctgcctg aaaatcaacg gcgagctgta
    12961 tattgttgcc aaccgtcacc tggattactt ccataaactg aagaagattt tcggcaactg
    13021 caccaccatc gccacgaata ataaatttgt ggtgctgaaa gcagtgaagc tggggcgtcg
    13081 tcggtaagat tgggagttac ctgccggatg cggcgttaac gccttatccg gcctacgttc
    13141 agcacgttac tcccaggccg gataagacgc ggcaagcgtc gcatccggca atgtctgccc
    13201 agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt caccatcctc tacattctct
    13261 tgtttagcgt tgttcaacgt ttatgctaca tacgtgaagt tatgacgcat aaagaatacg
    13321 atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    13381 gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc agaacgagga
    13441 acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    13501 cttgctggat ctggtgtgtg caaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    13561 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    13621 ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat ctatggtgtc
    13681 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    13741 attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg cgttgcatcc
    13801 ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg caattgcccg
    13861 tcgtgcgtcc agctctatag ccacatcgca gccgccaatt aaatgcacag ttttcccgct
    13921 atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga tcaccacatt
    13981 gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt catcgtcaat
    14041 cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg tggtgcgatg
    14101 gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc gctggagcat
    14161 cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc caccagcctg
    14221 ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct ggctggtgga
    14281 ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac aaccgatgat
    14341 ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac tcaatacttt
    14401 cggatgatcg atcccgtcga taggcggaac gcgcggcacg atcccactgg cgaggatcgt
    14461 ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca gcgtcacgcc
    14521 cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg taaaactcct ctttgccggg
    14581 gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgggcatcaa acaatgttac
    14641 ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc ccgcaccgac
    14701 caccgccaga ttttttttct gcacggcagg aaggattggc attttggttt cgtggcaggc
    14761 gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga ggcaggcctg
    14821 attgcagcca atgcaggtgt tgatctcatc ggctcgcccc gattgcgctt ttgacagcag
    14881 ttccgcatca gcaagaaacg gtcgtgccat cgataccata tcggcatcgc cgcgcgagag
    14941 aatgtcgtcg gcaacctgcg gatcgttaat ccggttggtg gtcaccagcg gcagcgaaac
    15001 gtggcctttc agtttgcgcg tgacccaagt aaatgcgccg cgcggcacgg gcgtggcaat
    15061 ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg cgcccgccgc
    15121 ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt cgaccaggtc
    15181 gagcatcgac agtcggtaga taataatgaa gtcgttgccg acgcgttcgc gcaccgcacg
    15241 cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact ggtcactacg
    15301 ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggacc ccattacctc
    15361 tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat cgtcgatcag
    15421 ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg gggcctgcaa
    15481 tgcggaggga gcgaccagat gcggttggta gctgtagcgc ccggtatgca aaatttgcag
    15541 ggctattttg ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt gtgggatctg
    15601 gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat ctggtgcaat
    15661 gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa acgctgccag
    15721 ccgctcggca ccgtccgggt attcctccag cccggtgtgc attgagccca tcaacacgcg
    15781 gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt agctcataaa
    15841 attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt agccgtcgcc
    15901 ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga atacgcccgg
    15961 tggtaacgcc ggatgcgacg cttgccgcgt tttatccggc ctgggggatt gcacatgtag
    16021 ggcggataag gcgttcacgc cgcatccgcc agtggcacgg tgcagatgcc ggatgcgacg
    16081 cttgtcgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    16141 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca
    16201 aatgcgcggc actccaggag aaatttggtg cgccttgctg tgcgccagtc agcgggttgt
    16261 aattctcctg aattgggcca tcggcggtta atcctttggc gtgacggaag aacgtatccg
    16321 ccagtttcag ggcatcatca cgataaccgt aacgctccat ccctttcaga ccaaaccaga
    16381 actgatccac ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc ggattggtta
    16441 acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca
    16501 ccaccgcgtc ggcgttggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg
    16561 gccctttacc gcgctcgaca atcggtttgc ccgcgcagcc gttcgccagc ggtttatctt
    16621 caatacgcac gtcatagtag aactgggtag ccgggtcgaa catacaggtg tttatgtagt
    16681 ctgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttccggctta ccgaggatgg
    16741 ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact
    16801 cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca
    16861 ccgtccagtc gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga
    16921 taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg
    16981 gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag
    17041 actgcgtctc ttctttgttg cctttcttca ccgtaaacag catctcgccg ctctcagtgt
    17101 tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt
    17161 tacgtaacca ccagtcgtga taggccacca gtttcgggta catctctgcc aaccaggctt
    17221 tatcctgggt gacgttgtac acttccatca ccgaccaggc ggcaaggctg ggtttggtat
    17281 tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca
    17341 ggtcggggac aaagcccaca tcctgcggac gcacgctatc gccaggctgg atctgccagg
    17401 agaagaccgc gcggatattc tctttggcga tgtccggatt gaaatgcgcc atcgcaaacg
    17461 cctgcttcca ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg
    17521 acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg
    17581 tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc
    17641 ctttcttcag atactcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg
    17701 ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagaggtc agcagatggg
    17761 aataggtggt atagagcgtc gtcgaaccgt tgatatgtgc cttactggta aagcgattgc
    17821 cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg
    17881 tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg
    17941 cgctgatttt gcgctggtag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt
    18001 tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt
    18061 tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca
    18121 tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg
    18181 cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc
    18241 tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg
    18301 tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa
    18361 aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa
    18421 tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc
    18481 aggttactgg cgttaaaata gtttttattt tcatgaaatt acagtcctta ttaccagtag
    18541 aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag
    18601 cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc
    18661 atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg
    18721 gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca
    18781 cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag
    18841 ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg
    18901 ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga
    18961 taggtgtatt tgtcgtcata gcgatcgtac ttgccaaaat gtagccaggc tttgctttcg
    19021 tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa agttgccgct taagttctgc
    19081 accttcagcg cggtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg
    19141 aagtcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt
    19201 tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca
    19261 ccccagctat ggtctggcac ccaggcattg ccgtcgtggt cggcctgcac ggtccagcct
    19321 tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct ccaggccacc ttcaatatag
    19381 gtaccgtcgc tgtatttatg tttattctca ccctctaaat ccatatatcc accaacccct
    19441 aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcagg tgtttttgtg
    19501 tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta gcaaaacggc
    19561 agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat ccatgagcta
    19621 aagaaaaaat acagcctttt atcgttgtaa ttataaaaat ataaaggcag aaactatttt
    19681 ccctctcctc atccaggaag agggaaaatg cctgctcatt ttttattatt ctgcttaatc
    19741 aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc cctttatata
    19801 tttactgtat ttccaccacg caaagccaag gaagataaca ataccgccga cgttataaaa
    19861 aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc ctacggcaaa
    19921 tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca tacggaaatc
    19981 gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga tgaataacgg
    20041 cggaagcatg gacgctgcgg cggtcatatt aataatagta ttcatcagat cctgcacggt
    20101 attggaaccg agcatcggga taatcatcag cgggatgacg atcaggaact ggatccacgc
    20161 tgcgcgcgcc ggaacgccgt tttcgttcag ttcgacggtt ttcttaccaa agatgccttc
    20221 cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca gggaaccgaa
    20281 catcgcggta aaggacacca gaccgacaaa gcgattcatc aacgcttccg gtagaccaaa
    20341 atacgccgcc atgccgtgga atacctgcac cgatccgccg gtaaatttca actctttgct
    20401 gctgacgaag acgttaatca gcaccgagga gacggaatac agtacgccga taaaaatccc
    20461 ggcgaggatg atcactttaa cgaacgattt cgaaccgcct ttgacgtcgt taacgtacac
    20521 agcgacggac tccgcgccac ctgcggccat aaagatccag gtggtaacgc cgaggaacgc
    20581 ccagttgaag ttcgggatca tcgcgtcaac ggtgatgggg tcagcaggct gtacgccgcc
    20641 aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt aacagcagca tcagcgttga
    20701 agtgacggag gtaattggcc ccaacatttt cgccccgttg gtggaaaccc aggtggagaa
    20761 ggcgaacagc accatactga gaatggtggt ggcaaccggc gtcataatat attcgtatcc
    20821 gaggaaggca tacgaagcat aagcaataac gcgcggcaac agtgaggtga aaaagaacag
    20881 gtttacgaac cagtaggtat aggcagtaat aaatgcccaa cgtccgccca gcgaactttt
    20941 tacccacgcg tagacaccgg cttctgagtt tttatttaac gaaacaaatt ctgcgatgat
    21001 cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa aacatcggtg ccgaggccag
    21061 tccaagctca atattattat tgataacgtt gttaaagctg taaacggcgg caaaagtcag
    21121 cgagagcaag ccgaatttgc cgattgtatt acgtttggta tcagacataa cacatctccg
    21181 aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc gccgtagttg ttatttgtta
    21241 tggaaataac catcttcgat ggtgactttg agaaccactt ttttgaccgc gttattgcag
    21301 ataaaccgat acgcttcatg gatatcgcaa ataacgattt gcccttcgtg gacctcaacg
    21361 gtttctccgc agccttttaa atattcacgg tcagtttcat cacgataata ttccactacc
    21421 tgtaatgtct ctttcggtgc atattcaatt ttttgctgcc cttgcaggta gtaatgcact
    21481 tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg cggagtctgt ctccacgcgg
    21541 taagtcaatg agtcaccgat ggagtgggcg acgccaggct gaatgttgtc gatattttca
    21601 atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt aaatctggcg gaactgttct
    21661 aagttatcga tgatcctcat tgcttattct cgctgtgcaa attcgtggaa aagaaccctg
    21721 cgcctaactc atacgacgcc aggctttgcg cggtagcttc tccgccagaa accggcagca
    21781 acgtaaagcc gtagctgaag tcacggaacc agacgcgcca ggagtccagc acctcgctgc
    21841 cccaggagtt ggagccgagg ccaagtagct ggtggtcgag gttcagggtg atgtcatcac
    21901 tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt ttcctgggta tagtgccagg
    21961 cgctgaagtt aattgggcgc tgcggaacca ccagcagacc gttgccgtgg cggttagtca
    22021 gtgccgtcca gcggacatgc tgacggttgc cgttgttctg cgggaaggga tagttctcga
    22081 acatggcatc gacggtgctg cgccagatat cgatgatgtt agcctgctgg ctgtcggcgt
    22141 agttttcgcc cggtccacga ccgtaatacg ccacctgatc gtattcgccg ttaatcccca
    22201 tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc gtaacgctcg ccggaaagcg
    22261 ccacgttaac ctggccatcg gcagcgatgc gccagatgta ggtgcagcgc atcccgaagt
    22321 caaacaccgg cggggcaata actgtgcggc tgatgatcaa cacttcacca tcgctctgtt
    22381 ctacggcaaa gtcgcgcaga tgttcctgca tgatctgcaa atgattcggt tgccacagcc
    22441 cttcgttgtc ctgcttgtgg ttgtcgatca ttggcttgaa gaagttgatc tttggctcgc
    22501 gggtcagcag cgactcgcca ttcacctgcc aggatgtcgg tttgccactc atttttgaga
    22561 aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa acgatcgtct tccagcgtca
    22621 gcggacgcgc attatttggt gcgaaaggca ctggttgcgc ggtgttttcc ttcagcggga
    22681 actgataagt ggcgatagaa tgcccggctt cgctgtagcg ggtgcgggaa tctttggtca
    22741 ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg cagcgtgatc tgcaaggggg
    22801 cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg cgtcgcgagc gtttcacctt
    22861 cgacgcgcac ctctgcgtgc agggtgtagt catcaagcgt ggtaaaccac agtttatttt
    22921 cgactttcag ctcgccgcga gtcagatcca gcgcgtggat ttttaccggc gcgataacct
    22981 gtttgtactc tttcaggccc ggtcccggcg tctgatcgga atagatcaaa ccatcaagac
    23041 agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac cagacgttgc
    23101 cgttgtcatc ctgcgcctgg atcccgtggt cgcaccactc ccaaacataa tgtccctgaa
    23161 tacaatcgtg cttatagaag acgttctggt actccgtcag cccgcctggt ccgttcccca
    23221 tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca ccaaactcat
    23281 tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca gcatcgcgat
    23341 cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt cgctgcgtgg tacatcgcgc
    23401 ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc gacggatggt
    23461 ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta gactttttcc cactgcggat
    23521 cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg acgtcggttt
    23581 ccgccatcac aaacaagccg tagatatcac acagttcgta aaaacgcgga tcgttcgggt
    23641 agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga tctttctcga
    23701 cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg ttgacgccgt
    23761 gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg atatcacgga
    23821 agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc agcgtcatga
    23881 ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg acagtaaaag
    23941 caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg ctgtgcacca
    24001 cgcgttcgcc atcaaacaag gtatattcca gcgtcgtgac gacaggggag gcggcgagat
    24061 tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca aagtcggtac
    24121 gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca tcgcggaaga
    24181 tccccgctga ccaccacata tcctggtctt ccacgtaggt agagtccgcc cactgcatca
    24241 cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca aactctgcgg
    24301 tcaggcgact gcccttgctg aaacccacat actgaccgtt aacatagact tcaaaatagg
    24361 tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg ctgagggtga
    24421 aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca tcgatgggga
    24481 acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc cacatggcgg
    24541 ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct ggtacttgca
    24601 gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac aggctgctgg
    24661 tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag tacgcacgcg
    24721 gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata gggcctcccg
    24781 gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct ggggagttta
    24841 gtaaaatttt tactaaaaag tagcaggtaa atgcatttac tttaactgga tcacgaaaat
    24901 aaacggcagc actgtccggc aaaaacgatt gccggacagt gtgtaagggg atttaacggg
    24961 tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa cagcggcagc gcgcgaccat
    25021 cgcgggcttt ttcatacacc aggttaacgc cctgacttcc catcatctcg gaatggatgc
    25081 gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg gatatcgtta acgctgataa
    25141 gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat tgcccgcagt acgccgatag
    25201 caatggaatc ggaagcaaca aacagtgcct tcggatagtc ttcccgcgcc agcatttgtt
    25261 ttgccagttc ataacccgac gaactggaaa aaccgccgcg ccagatatcc tcttcgcgta
    25321 ccacttgttt cagtcggcca tattccgcaa aggcgacctc acgaatatcc gccttgccag
    25381 gctcatcttc accgccaata aaaccaatac gattaacgcc ctggttgata tagaagtcga
    25441 tgatttcttt actgatgcgt gccagatcga tatccaccgc atcgtaaccg ctgccgggtt
    25501 cgtgaaagtc gataaaacag atattgtcgg tcaacgcgct ggcagcggcg cgcagggcgg
    25561 gcgtgggttt gccgacaatt aaaataccgg tgacgttttt aatgtctggt aagccgctgt
    25621 gttcataaca gttggtgagc tcgatgccca gcttttcgca ctgggtttca atgccgtggc
    25681 ggatcgccag atagtaagga tcgttgatct ccagctcctg ctggtagctg tagatagcca
    25741 gaatatggtg ttggttgact gcacctgtct ggagtttacg ggcactactg gtcttgtact
    25801 ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc ttctttcaca ttcaatgtcg
    25861 gatcgtcatt taagaccctg gatactgtcg ccagggatac gccagcttcg attgcgatgt
    25921 cttttagtgt tgccatgttt acctttttta ctttccctga tgtgcgcccc cattgtagag
    25981 gaagtgctta tcgggtcacc tgttttcagt aaataaagcg cgtttctttg acgctgtaac
    26041 ggtaacacag cggctcacga atgtgatcac gcctgcattc tccattttta gcctacagga
    26101 ttgagaaaaa cttttgccat agtttagtaa aattttaccc aaagggagga aacaatggaa
    26161 accgtggctt acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat tgtggaagtg
    26221 aaacgccatg aaaacgccga caagctgtac atcgtacagg ttgatgtggg ggaaaaaaca
    26281 ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag aagagctgat ggggaaaacg
    26341 gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg gtgaaacgtc ggaatgcatg
    26401 ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgt tgttaacgcc ggagcggatg
    26461 atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg cgacgtcgta tcgccatccg
    26521 atttgatatt acgcttcttc gacacttact cgcatggccg ccagcgcctt acgcgccgct
    26581 ttgatcacca gttcacactg ttcaatggtc agtgtcagtg gcggttcaat gcggatcgtt
    26641 ttggcgttat tgagcgttcc ggccaccagt acgcgctggc ggaacatctc gctggcaaag
    26701 ttatagccga tttcgttatc aacaaactca attgccatca acatcccttt accacgcgct
    26761 tcctgtacca gatcgggata ttcccgcgcc agttgacgga aaccgtccag caacatatcg
    26821 cctttttgct cagcctgagc cggtaagttc tgctccagca acacattgat ggtcgctagc
    26881 gccgccgcac aggccagcgg gttgccgcca aaggtggtgg tatgcaggaa tgggttgtcg
    26941 aacagaactg aaaacacctc ttcagtggcg atggtcgcgc caatcggcat cacgccgccg
    27001 ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt tctcatgctc gcaggcgaac
    27061 atcttgcccg tgcgccccat gcccgtttgt acttcatcga ggatcatcag tgcgccgaac
    27121 tcatcgcata gcttacgtac ggcggtgaga tagcccggcg gcggcagaat tacgccacct
    27181 tcaccctgaa tcggttcgag gatcaccgca gccacatcat caccggtttt tttgcactcg
    27241 ttaagagccg tgcgcatggc ttcgatattg ccaaacggca catgacggaa gcccggcagc
    27301 agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg ccgacagcgc gccaagtgat
    27361 ttaccgtgga acgcgccgct ggtggcaata aaagtaaact tgccgcgcgg tgactggtaa
    27421 gctttcgcca gcttcagcgc tgcttcaacg gactcggtgc cgctattaca gaagaagctg
    27481 tatttcagtt taccgggcgt tagcgcagca agggttttcg ccaacatcgc ccgtaacgga
    27541 tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt gattctgtac ggcggaaacc
    27601 acaactggat tacggtgccc cacgttgaaa attccaaaac ctcccaggca gtcgataaac
    27661 tcctgtccct gggtgtcgac aagcgtattt aaacctcccg cttgccactc tacggctccg
    27721 taatccccgc cggcggtaac agatttgcga tactctaaaa accccggatt gacatgctct
    27781 ttgaagtatt caatcacctc tcggttaagt gctttcatct cctcatgatc cagcgttcgc
    27841 ttctcaatga gattcagggc gtgggcgctg cacgctaaag ccgatgcgct cgaaggtaac
    27901 ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat accgatttaa gtattgcagg
    27961 gattgcgcca ctccggctgc gatcgcgaaa atcgggataa acacaaattc ggttatcatt
    28021 tgtgcaatat ttaatgctat ctgttaacat ttgttgttat tttgcacggt ttttgtcggg
    28081 tatggatttg atgattgctg cgcaaatgca cgttttcagg gcaataattg cctgattttt
    28141 gggcaagata attaacgttt tcgtatagta acagagtgaa agtattttaa atcaaagaat
    28201 tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa ttaatcggtt aaagataacc
    28261 gcagcggggc cgacataaac tctgacaaga agttaacaac catataacct gcacaggacg
    28321 cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac cccgctggcg gacgatacca
    28381 ctctgatgtc cactaccgat ctgcaaagct atatcactca tgctaatgac acttttgtgc
    28441 aggtgagcgg ctttaccttg caagagttac aagggcagcc gcacaacatg gtgcgtcacc
    28501 cggatatgcc aaaagcggcg tttgcggata tgtggttcac cctgaaaaaa ggggagccct
    28561 ggagcggcat cgtgaaaaat cgccgcaaaa atggcgacca ttattgggtg cgggccaatg
    28621 cggtaccgat ggtgtgcgag ggaaaaatca gtggctatat gtcgattcgt acccgggcga
    28681 cggatgaaga gattgcggcg gtggagccgc tgtacaaagc gctgaacgcc ggacgtacca
    28741 gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg gctgggtaaa ctgccttcat
    28801 taccgcttcg ctggcgggcg cgtggagtga tgaccctgat gtttatcttg ctggcggcca
    28861 tgctttggtt tgttgctgcc ccggtggtga cgtatttcct ctgtgtgtta gtggtattgt
    28921 tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat agaaaatgtc gcccgtcagg
    28981 cgctgaaggt ggcgaccgga gagcgtaata gtgttgagca cctgaatcgc agcgatgagc
    29041 tggggctgac attacgcgcg gtagggcagc ttggcctgat gtgccgttgg ctaattaacg
    29101 atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga gacgctggcg aaaggcaccg
    29161 atgaactgaa cgaacatacc cagcagacag ttgataacgt tcagcaaacg gtggcgacca
    29221 tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac ggcgtcggct gccgataaac
    29281 tttctatcac cgccagtaat gcggcagtgc agggtgggga ggcgatgacc acggtgatca
    29341 agacaatgga cgatatcgcc gacagtaccc agcgcattgg caccattact tcgctgatta
    29401 acgatattgc gtttcagacc aatattctgg ccctgaatgc ggcggtggaa gcggcgcgtg
    29461 ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga agtgcgtcat ttagccagcc
    29521 gcagcgctaa tgctgccaac gatattcgca agctgattga tgccagtgct gataaggtgc
    29581 aatccggttc gcagcaggta cacgccgccg gacggacgat ggaagatatt gtggcacagg
    29641 tgaaaaacgt cacccagttg atcgcccaga ttagccattc aacgctggaa caggccgatg
    29701 ggctttccag cctgacccgt gcagtggatg agcttaacct gatcacccag aaaaatgccg
    29761 agctggtgga agagagtgcg caggtgtcgg cgatggtgaa acaccgcgcc agccgactgg
    29821 aagacgcggt gacggtgctg cattaatcgg tatgccggat ccggcggttg gagcacaatg
    29881 cctgatgcga tgctggcgca tcttatcagg cctgcggggt gtgtagcggg tgtaggccgg
    29941 ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgat
    30001 taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat cttttttatt
    30061 tgtgcatcac tgatatcgct ctggttgacg tgtaaatcca gcaccgcttt aaaattatcc
    30121 agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg cgctttgatg
    30181 cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgcgc gccttgttcg
    30241 gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag cgactgctcc
    30301 tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg gggtgtaatt cccctgggtt
    30361 aggttctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga gagaatatcc
    30421 agaatcacca gacgcaattc accgtgacca aagaaacgtt gccgacgacc gccgccgcga
    30481 ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg
    30541 tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc atgtttacaa
    30601 cacccttcgt gatgatggct catatcagcc ttcttttgct tgattagata tatctaattt
    30661 atatctgatg tgagcattac tgcaagtaca aaaatgattt ttaaacgtgg aagcacctca
    30721 catccaggcc ggataagacg ttcacgccgc atccggcagc attgctatgg cactgaatgc
    30781 cttcacatac ttcagatata tcagttctac aaatcgcttg catttatcat gattaacaat
    30841 cattatcatt tacgaaaatc atttagatat atctgattac tatcacgaag gcgataacaa
    30901 tgaataactc cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc cgtgaactga
    30961 ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctc ggcggcgagg
    31021 cgctggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc tttcctcaac
    31081 ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg ccggaaggac
    31141 cacgcccact gtcgcgtgac tatacgccgc tgtatgacga actacgccat gaactggcga
    31201 tcgatttctt tattcacgac ggtggggtct ccagcggctg ggcgatgcag gcgcaaccgg
    31261 gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt
    31321 atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt
    31381 tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct
    31441 gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc
    31501 aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga
    31561 taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg
    31621 acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga
    31681 aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa
    31741 ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag
    31801 cggattataa agtaactccg tgtcgcaatc cagccattac cgtgcgcctg ctgtttttat
    31861 aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg agtaaatatg
    31921 gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt
    31981 tcatccgccg ggactggttt tccctttgct catccagcaa atcgcttctg gaaggtgata
    32041 tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat
    32101 tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca
    32161 aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga ttatcagccg
    32221 caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcca gcgcggtgca
    32281 cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc
    32341 agcggtttaa gtcgcgtttc actagagaaa ctggttgaag catatcgcga gctggaccag
    32401 gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa ggtggcgttt gtgcaaaggg
    32461 actagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt ccaggaagct
    32521 acgcaacact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg cttcgatctg
    32581 acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg tgtggtcggt
    32641 gttcatatcg ataccgaaac gcatacgcag aactttcgct tcacgcgcgg tcaggccagc
    32701 cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca gcggcagctc
    32761 gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac cgatcggcgt
    32821 ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt cttccggcat
    32881 cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt gcagcatctg
    32941 gcgagaaata cggttgagtt tgttgatagt ctcaatcata tgcaccggaa tacggatggt
    33001 gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg atccaccagg ttgcgtaggt
    33061 ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca gaccgatgtt
    33121 gccttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct tggcgataga
    33181 aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg ctttcgcttc
    33241 accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc cggtttcttc
    33301 ttcaatctgc tgcagtttct gcagggcgcg atgcacttct tcagagacat cgtgcagttt
    33361 ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc tggtttcgtt
    33421 gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa cgcagagctt
    33481 catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc tgttgaccag
    33541 gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca gtttcaggat
    33601 ctcttcctga gcggcagcgt gactgcgacc tttcgctttg atggtgtcac gcgttacaac
    33661 gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga tgctgttgtc
    33721 atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat cgtccagatc
    33781 ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt cttctgcgtt
    33841 cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt caacacgatc
    33901 gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc attgaacctg
    33961 gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg tcaacagttc
    34021 aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc cgatttcaga
    34081 ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg cggtgttttc
    34141 agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc ccatgtcgtt
    34201 gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg gcagatggtc
    34261 attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa gaagtttcag
    34321 ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg aggttggtgt
    34381 tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt tgtttaccgc
    34441 caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg cggctgtcgg
    34501 gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc cagctccagg
    34561 tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg ctcacgagcg
    34621 attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag tgagtcggtg
    34681 aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag tttttcaagg
    34741 gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt cagacctggc
    34801 tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag cttattttca
    34861 tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac cagcaaccct
    34921 ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac gccgctctct
    34981 gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa tttgttgcct
    35041 aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat caatggcagt
    35101 gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg cggcatcagg
    35161 ctgttaaaca gaaatgcgga gagtggcatc gcctgctcca tccgcgcttc aaacgcttct
    35221 ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat ccggcaaaaa cataaagcgt
    35281 agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca tgcggcatcg
    35341 cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg gaacaacagt
    35401 tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt aatgccgtat
    35461 tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt gggttcagcg
    35521 ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa aatgtctgtt
    35581 tccggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc aaaaccaatc
    35641 acccgaccgc gtttatcgcg aatggggaac atcacccgct cgcggaaacg atcgtaactg
    35701 cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg attttctgga
    35761 ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc aatcgcaaag
    35821 cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc agacgtggca
    35881 acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa ctgataaagc
    35941 gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa tggcacttca
    36001 agattgtgca ttgctgccag ctcttcgacc gtttcaacga actcgagctt gtcgtagttc
    36061 atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta aaactgtttc
    36121 tcaccgttaa cggtgaatga cggggttttc tcgttgtgga atggacaaca cgcgtggaaa
    36181 ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat gtcagtgcgt
    36241 gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc cgatttttag
    36301 caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt acaactacaa
    36361 ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg cggcgtgcgt
    36421 tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg cgcttacgtt
    36481 cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca cctgcttttt
    36541 cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca cgtactttaa
    36601 ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat caacgccagc
    36661 ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa gcaccgcggc
    36721 cttttttgaa agggactttt ataagggtga ggagtataca cgaggctttc tccaggggcg
    36781 aacaaagttt tacatcaacc cgcattggtc ctacactgcg cggtaataaa gcgaggtaaa
    36841 acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat gaaaccggca tcgccattta
    36901 cgacgatgaa aaaggtttgt tagccaacca attgtatagt caggtgaaat tgcacgctga
    36961 ctacggcggc gtcgtgcctg aactggcctc ccgcgatcac gtgcgcaaaa ccgtaccgtt
    37021 gatccaggcg gcgctaaagg agtctggttt aacggcaaaa gacattgatg ctgtggccta
    37081 taccgcaggc cctggattag tcggcgcact gctggttggc gcaaccgtgg ggcgttctct
    37141 ggcgtttgcc tggaacgttc cggcaatccc ggtacaccat atggaagggc atctgttagc
    37201 gccgatgctg gaagataacc cgccggaatt tccgtttgtc gcgctgctgg tgtccggcgg
    37261 tcatacgcag ttaatcagcg tgactggcat tggtcagtac gagctgctcg gcgagtctat
    37321 cgatgatgcc gccggtgaag cgtttgataa aaccgcgaag ctgctggggc tggattatcc
    37381 tggcggaccg ttactgtcga aaatggcggc tcagggtact gccgggcgct ttgttttccc
    37441 gcgtccgatg accgaccgtc cggggctgga tttcagcttt tctggtctga aaacctttgc
    37501 ggcgaacacg attcgtgaca acggcaccga cgaccagacg cgtgctgata tcgcccgcgc
    37561 ctttgaagat gcggtggtcg atacgttgat gattaagtgt aagcgagcgt tggatcagac
    37621 gggctttaag cgactggtca tggcgggcgg cgtgagtgct aaccgcacgt tacgggcgaa
    37681 gctggcggaa atgatgaaaa aacgccgcgg cgaagtgttc tacgcgcgtc cggaattttg
    37741 tactgataac ggcgcgatga tcgcctatgc cggaatggtg cggtttaaag caggcgcgac
    37801 ggcggatctc ggcgttagcg tgcgtccgcg ctggccactg gcagagttac cggctgcgta
    37861 aaactatccg gtgccggaga gcaatttccg gcaccgtcct cacttaaagc aacaccacgg
    37921 gcatccacag caagccggta atcaccagca acacgaggaa tatcagtcca aaaatcgccc
    37981 ccagtcgcca gtaatccacc gttggcagat aaccactacc gtagtaaatc ggactgggtc
    38041 cggtggcgta tggtgtaaga atgctcccca gaccaattgc cgcgccaacc atcaggcaga
    38101 acaccggcag cgggatttcc ggcatcgcca gcgcagcggc aatcatcatc ggtgcgaggg
    38161 cggaggtata cgccgtggcg ctggcgaaaa agtagcgcag cagataaaac accacaatca
    38221 acgccaccat caccatcgtt ggcgaataac cgcttaagct gcctgctaac agtttgccaa
    38281 accagctaat aaaaccagtg ttgttgagtc cggtagcgag ggtgataagc gaggccagcc
    38341 agaagaaaac gttccacgcc gctttattac tgacaatgtc gtcccaactg ataatgcgca
    38401 gaagcagcat cagtgccacc acgctgtaac cgaccatcgc ggcatcgata taatcaccgc
    38461 cgaaaatcca cagcaccagc gcgcctacca tcagccccag catccgtttt tcacgcgaac
    38521 agagcgggcc cattgcctgc agttccgtct ctgcccagcg cggcacctga tcgccagact
    38581 tcagtaccgg cgggtacagc acataagcca gccagggaac cagcagcacc agcaaaatac
    38641 tgagcggcaa catcccgagg aaccagtcgc cccaactcag cgtggcgtga gatgcgcttt
    38701 tcatcagtcc aattaacagc aagtttggtg ccatcgccgt caagaaaatg gcgctggtca
    38761 cgcagtcggc aacaatcccc atccacatga tgtacgagcc aatgctgcgc gaactgctgt
    38821 cgtttggttg tgattgatag agcggtggca ggttacggat gatgggatag ataatccccg
    38881 caccacgcgc cgagttggac ggtgttacag gtgcgaggat cagctcggag aacatcaccg
    38941 catagccgag aaacagcgtg cgatgtccca tctttttcac cagaaacagc gcaatgcggc
    39001 gtccaagccc ggttttttca tagcctgtgc caaacataaa ggcggcgaaa atcagccaga
    39061 taaccgaatt agaaaaaccg gaaaccgccc acgagaggga ttttgcagtg aatttaaagc
    39121 ctggctgggc gagctgttcg gggctgaaca gcagccaggg agagagtatg gcgatgatgg
    39181 agatacccac catcgccacc acggcacccg gcacgggttc gaggatcagt ccaacgatca
    39241 cgccagtaaa aacggcaaag tagagccagg tatggttctc cagccctgcg ggaaccggaa
    39301 gtagagcaat aatggcgatg accgccagcg gcgcaagata tcgccaccat tcagtggaag
    39361 gtttcatcgt gttatgcctg cgaaaaagga gaggggtgag cctctccggg agggttattt
    39421 gatgtaatgg acgtgctcgc agatctcttc cacgatggga tcgcggcgtt cggcgaacag
    39481 ctttttgttt tcggctatca ggttgttgcc gtgggtatca atagagacaa tcagcgggcc
    39541 gaactctttg acccggcaga cccacagtga ctccggcatc ccgagttctg tccagtgcac
    39601 ttcttcaatc tcttctacct gggttgccgc cagcaccgcg cagcctgccg ggaaaatcac
    39661 atgtagcgcc ttgaatttct ggcagccttc ttcggtcagc ggccccatac cacctttgcc
    39721 aaccaccagt ttcacgccgg tctgctcgat aaattcacgt tcaaaacttt ccatacgcat
    39781 actggttgtc gggccaacgg aaaccatctc ccatttatcg ccgtttttgc gcacgatggg
    39841 gccagcgtgg aaaatcgctt tgccgttgag atcgtaaggg atcggacgtt tcagttcgat
    39901 caagcggcgg tgacaaacgt cacggcaggt caccagcgta ccggtcaggt agatcacatc
    39961 gccgacgcga atatcttgca gatcttcagc tttgatcggg gttgtcagga tctttttcat
    40021 aacgcgctcc gggtgtgaga cagattttca aaggtgagat cggcatgaac cagcagtgtg
    40081 ccgcgacgat gcgcccagca gcctgtagaa acagcaacgc cgatggttga cggatggcgg
    40141 gcggcagatt cgatatgcac gcccatcact gaactgttgc cagtcagccc ttgtgggcca
    40201 attcccagac ggttgagtcc ttcttccagg cgtagctcca gctctgccgc ttttggattg
    40261 ggatggcggc tgccaatcgg gcgcaaaatg gcttttcgcg agagcacggc ggcggtttcc
    40321 accgaggtgg cgatgcccac gcccaccagt accggtggac aggcgtttac ggcgagggtg
    40381 gagatatttt cgaagacgaa tttcaccacg ccttcgtagc cttctgacgg cattaacact
    40441 ttcgagcggc caggtagcgt gcagccgccg cctgccatgt aaacttcgat ttccgcatcg
    40501 tcattgtcgg ggatgatgtc ccaggtgacc cacggtacgc cgctaccggt atttttgccg
    40561 gtgtttactt cgtcaaaaat ttctaccgca ttgtgacgta gtggcgcttt gacggttgcc
    40621 tcttccacgg cttgtttgag tatgctttgc agctcgccaa gcagtgggaa gcgggaaccg
    40681 actttaacga agaacataat ctccccggtg tcctgacagg caggacgatt caggtcaatc
    40741 gctttttgca tgttgtcgaa catcgtatgg tagataattt tccccatcga cgacgtttcg
    40801 gcatccttta gctgttttag tttatccacc acgtcatcag gcattcgggt agaaatcatg
    40861 gcggtaaagt tagcgacaat ctctgtcaac ttattcactg cctgttgctt attactttcg
    40921 ctcatcatgg caactccagt ttttgtattt gtctggaaaa attcaccgcc acaaactacc
    40981 actaaaaatg tggttttata ttttttgcca ccgtgaatat gtaattaaca gttcgtgaat
    41041 caacgcgaaa ataagaatga atattgcgcc agagcaggga atggcaatgt tttgcgaaat
    41101 acactgcgct tatcacaact gcggataatt aacgatgctg aatagctggc ctttagccaa
    41161 agacttgcag gtactggtag aaatcgtcca cagcgggagt tttagtgctg cagcggcgac
    41221 gcttgggcag acgcctgcat ttgtcaccaa gcggatccaa atccttgaaa atacgctggc
    41281 aacaacgctg ctgaaccgct ccgctcgcgg cgtggcgctg actgaaagtg gccaacgttg
    41341 ttacgaacat gcgctggaaa tcctcaccca atatcagcga ctggtcgatg acgtcacgca
    41401 aatcaaaacg cgcccggagg ggatgattcg tattggttgc agttttggtt ttgggcgcag
    41461 ccatattgcg ccagccatta ccgaacttat gcgcaattat cctgagttac aggtgcattt
    41521 tgaattgttc gatcggcaaa ttgatttagt gcaggataat attgatctgg atattcgtat
    41581 taatgacgaa attcctgatt attatattgc gcatctgtta acgaaaaata aaagaatatt
    41641 atgcgcagca cctgagtatc tgcaaaaata tccgcagcca caatcattac aggaattaag
    41701 tcgtcatgac tgtctggtga ccaaagagcg cgatatgacc cacggaatat gggagttggg
    41761 gaacggtcag gagaaaaaat cggtgaaggt tagcgggcat ctttcctcca atagcggcga
    41821 gatagtgttg caatgggcgc tggagggcaa agggattatg ctgcgttccg agtgggatgt
    41881 gttgccgttt ctggagagtg gcaaactggt gcaagtattg ccggagtatg cacaaagcgc
    41941 caatatctgg gctgtttacc gggagccgct ctatcgcagc atgaaattac gtgtctgcgt
    42001 ggaatttctg gcggcatggt gccagcaacg gctgggcaag cccgataaag gctatcaggt
    42061 catgtagatc caccagaaat cactcgggat ccttttcgcg ctttcttttg aatttcgtcc
    42121 agatttttgt ctcctgacga cgccacagac gctggatgtt gtcatgatga cgcagcagga
    42181 tcaggcaaga gagcatcgaa accgggaagg tgaattgtgg cttaaaccac catacataaa
    42241 acggagcaat cagtgcactg acaatcgctc ccagcgacga gtatccgctc aatagcacgg
    42301 tcagtaacca ggttcccgcc attactccgg tgagatccca gccaatgggt gcgatggcac
    42361 caaaagcggt agcaacgcct tttcctcctt taaatccgaa gaaaacgggc cagatgtgtc
    42421 caagacaggc ggcgatggca attaagccta gccagaaggg gctgacacct aattcatacg
    42481 cgccccagac gggcaacatt cctttcagaa cgtcgaaaat cagtactgct acggctgctc
    42541 ccttgccacc gatacgtaac acattggttg cgcctggatt gccggagccg ctggttcgcg
    42601 gatcgggcag cccacacaag cggcaaacca gaatggcact ggaaatggag ccgcagaggt
    42661 acgcgatgag gatcattcca ggcgcgattg cactcataag ctgttccgtt ttgaaaattc
    42721 gtgttcaacg atgaatctgt ggataatacg cacatttcgc cggaagtggt atccggttag
    42781 ccaaaaagca ggcaggacgt gatggatatt gtatttatag agcaactttc ggtaatcacc
    42841 actattggtg tttacgactg ggaacagacc atcgaacaga agttagtgtt cgatatcgaa
    42901 atggcatggg ataaccgtaa agcggcgaaa agtgatgatg tggcggattg cctcagttac
    42961 gctgacattg cagaaacggt ggtcagccac gtcgaggggg cgcgttttgc gctggtggaa
    43021 cgcgtggctg aagaggtggc ggagctgctg ttagcacgct ttaactcgcc gtgggtgcgt
    43081 atcaaactca gcaagccagg cgcagtggcg cgggcggcga atgttggcgt aatcattgag
    43141 cgtggcaata atctgaaaga aaataattaa ttttacagct gttaaaccaa acggttataa
    43201 cctggtcata cgcagtagtt cggacaagcg gtacatttta ataatttagg ggtttattga
    43261 tgagcgatat gcactcgctg ctgatagcgg caatattggg tgtggtcgaa ggattgacag
    43321 aatttctgcc ggtatccagc acgggccata tgattattgt cggtcacttg ttggggttcg
    43381 agggcgacac ggcgaaaacc tttgaagttg tgatccagtt aggatcaatt ctggcggtag
    43441 tagtgatgtt ctggcggcgt ctgtttggcc tgattggcat ccactttggc cgcccgttgc
    43501 agcacgaagg agaaagcaaa ggtcgtttaa cgctgatcca cattttgctg gggatgattc
    43561 cggcggtggt actggggctg ttgttccacg acacgattaa gtcattgttt aacccgataa
    43621 atgtgatgta tgcactggtc gttggcggtt tgttgctgat tgccgccgaa tgcctgaagc
    43681 cgaaagagcc gcgtgcgccg ggtcttgatg atatgaccta tcgtcaggcg tttatgattg
    43741 gctgtttcca gtgtctggcg ctgtggccgg gtttctcccg ttccggggcc accatttcag
    43801 gtgggatgct gatgggcgtg agccgttacg ctgcttccga gttctcgttc ctgctggcgg
    43861 taccgatgat gatgggcgca acggcgctcg atctctacaa aagctggggc ttcctgacaa
    43921 ccggcgatat cccgatgttt gccgttgggt ttatcaccgc ttttgtggtg gcgctgatag
    43981 cgattaaaac cttcctgcaa ttgattaagc gcatttcgtt tatcccgttc gccatttatc
    44041 gctttattgt ggcggctgcg gtgtacgtcg tgttcttttg attgagaaaa ggttcgggag
    44101 caggcctggt tttctccctc gctctgtagg gaga
//
LOCUS       DAZUFB010000030        44078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZUFB010000030 DAZUFB010000000
VERSION     DAZUFB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.030"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..796)
                     /locus_tag="ACHY3M_001886"
     CDS             complement(<1..796)
                     /locus_tag="ACHY3M_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4316812.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYL"
     gene            complement(1056..2309)
                     /gene="amiC"
                     /locus_tag="ACHY3M_001887"
     CDS             complement(1056..2309)
                     /gene="amiC"
                     /locus_tag="ACHY3M_001887"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFS4316813.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2541..3872
                     /gene="argA"
                     /locus_tag="ACHY3M_001888"
     CDS             2541..3872
                     /gene="argA"
                     /locus_tag="ACHY3M_001888"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFS4316814.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3934..5760)
                     /gene="recD"
                     /locus_tag="ACHY3M_001889"
     CDS             complement(3934..5760)
                     /gene="recD"
                     /locus_tag="ACHY3M_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFS4316815.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNEQTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHACNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5760..9302)
                     /gene="recB"
                     /locus_tag="ACHY3M_001890"
     CDS             complement(5760..9302)
                     /gene="recB"
                     /locus_tag="ACHY3M_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFS4316816.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLCTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARMLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9295..12183)
                     /gene="ptrA"
                     /locus_tag="ACHY3M_001891"
     CDS             complement(9295..12183)
                     /gene="ptrA"
                     /locus_tag="ACHY3M_001891"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFS4316817.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEV
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12359..15727)
                     /gene="recC"
                     /locus_tag="ACHY3M_001892"
     CDS             complement(12359..15727)
                     /gene="recC"
                     /locus_tag="ACHY3M_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFS4316818.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15740..16063)
                     /gene="ppdC"
                     /locus_tag="ACHY3M_001893"
     CDS             complement(15740..16063)
                     /gene="ppdC"
                     /locus_tag="ACHY3M_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFS4316819.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16048..16455)
                     /gene="ygdB"
                     /locus_tag="ACHY3M_001894"
     CDS             complement(16048..16455)
                     /gene="ygdB"
                     /locus_tag="ACHY3M_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFS4316820.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16452..17015)
                     /gene="ppdB"
                     /locus_tag="ACHY3M_001895"
     CDS             complement(16452..17015)
                     /gene="ppdB"
                     /locus_tag="ACHY3M_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS4316821.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(17006..17476)
                     /gene="ppdA"
                     /locus_tag="ACHY3M_001896"
     CDS             complement(17006..17476)
                     /gene="ppdA"
                     /locus_tag="ACHY3M_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS4316822.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVIVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17661..18455)
                     /gene="thyA"
                     /locus_tag="ACHY3M_001897"
     CDS             complement(17661..18455)
                     /gene="thyA"
                     /locus_tag="ACHY3M_001897"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4316823.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18462..19337)
                     /gene="lgt"
                     /locus_tag="ACHY3M_001898"
     CDS             complement(18462..19337)
                     /gene="lgt"
                     /locus_tag="ACHY3M_001898"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4316824.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19488..21734)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_001899"
     CDS             complement(19488..21734)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_001899"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4316825.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21747..22277)
                     /gene="rppH"
                     /locus_tag="ACHY3M_001900"
     CDS             complement(21747..22277)
                     /gene="rppH"
                     /locus_tag="ACHY3M_001900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFS4316826.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22630..22776)
                     /gene="ygdT"
                     /locus_tag="ACHY3M_001901"
     CDS             complement(22630..22776)
                     /gene="ygdT"
                     /locus_tag="ACHY3M_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFS4316827.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22962..23651
                     /gene="mutH"
                     /locus_tag="ACHY3M_001902"
     CDS             22962..23651
                     /gene="mutH"
                     /locus_tag="ACHY3M_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFS4316828.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23720..24433
                     /gene="ygdQ"
                     /locus_tag="ACHY3M_001903"
     CDS             23720..24433
                     /gene="ygdQ"
                     /locus_tag="ACHY3M_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS4316829.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24571..24789
                     /gene="ygdR"
                     /locus_tag="ACHY3M_001904"
     CDS             24571..24789
                     /gene="ygdR"
                     /locus_tag="ACHY3M_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFS4316830.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24897..25937
                     /gene="tas"
                     /locus_tag="ACHY3M_001905"
     CDS             24897..25937
                     /gene="tas"
                     /locus_tag="ACHY3M_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFS4316831.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25969..27162)
                     /gene="lplT"
                     /locus_tag="ACHY3M_001906"
     CDS             complement(25969..27162)
                     /gene="lplT"
                     /locus_tag="ACHY3M_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFS4316832.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(27155..29314)
                     /gene="aas"
                     /locus_tag="ACHY3M_001907"
     CDS             complement(27155..29314)
                     /gene="aas"
                     /locus_tag="ACHY3M_001907"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFS4316833.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29900..30931
                     /gene="galR"
                     /locus_tag="ACHY3M_001908"
     CDS             29900..30931
                     /gene="galR"
                     /locus_tag="ACHY3M_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFS4316834.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30938..32200)
                     /gene="lysA"
                     /locus_tag="ACHY3M_001909"
     CDS             complement(30938..32200)
                     /gene="lysA"
                     /locus_tag="ACHY3M_001909"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4316835.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32322..33257
                     /gene="lysR"
                     /locus_tag="ACHY3M_001910"
     CDS             32322..33257
                     /gene="lysR"
                     /locus_tag="ACHY3M_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFS4316836.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33244..33936)
                     /gene="ygeA"
                     /locus_tag="ACHY3M_001911"
     CDS             complement(33244..33936)
                     /gene="ygeA"
                     /locus_tag="ACHY3M_001911"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFS4316837.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(34065..35483)
                     /gene="araE"
                     /locus_tag="ACHY3M_001912"
     CDS             complement(34065..35483)
                     /gene="araE"
                     /locus_tag="ACHY3M_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFS4316838.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35798..36559)
                     /gene="kduD"
                     /locus_tag="ACHY3M_001913"
     CDS             complement(35798..36559)
                     /gene="kduD"
                     /locus_tag="ACHY3M_001913"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFS4316839.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36589..37425)
                     /gene="kduI"
                     /locus_tag="ACHY3M_001914"
     CDS             complement(36589..37425)
                     /gene="kduI"
                     /locus_tag="ACHY3M_001914"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4316840.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37712..38899)
                     /gene="yqeF"
                     /locus_tag="ACHY3M_001915"
     CDS             complement(37712..38899)
                     /gene="yqeF"
                     /locus_tag="ACHY3M_001915"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS4316841.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIE
                     RDE"
     gene            39154..40383
                     /gene="yqeG"
                     /locus_tag="ACHY3M_001916"
     CDS             39154..40383
                     /gene="yqeG"
                     /locus_tag="ACHY3M_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4316842.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLFSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            40843..41475
                     /gene="yqeH"
                     /locus_tag="ACHY3M_001917"
     CDS             40843..41475
                     /gene="yqeH"
                     /locus_tag="ACHY3M_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4316843.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPSLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            41809..42618
                     /gene="yqeI"
                     /locus_tag="ACHY3M_001918"
     CDS             41809..42618
                     /gene="yqeI"
                     /locus_tag="ACHY3M_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4316844.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            42611..43093
                     /gene="yqeJ"
                     /locus_tag="ACHY3M_001919"
     CDS             42611..43093
                     /gene="yqeJ"
                     /locus_tag="ACHY3M_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFS4316845.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(43126..43206)
                     /locus_tag="ACHY3M_001920"
     CDS             complement(43126..43206)
                     /locus_tag="ACHY3M_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316846.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(43242..43667)
                     /gene="yqeK"
                     /locus_tag="ACHY3M_001921"
     CDS             complement(43242..43667)
                     /gene="yqeK"
                     /locus_tag="ACHY3M_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFS4316847.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNTEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            43861..>44078
                     /locus_tag="ACHY3M_001922"
     CDS             43861..>44078
                     /locus_tag="ACHY3M_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316848.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQ"
BASE COUNT        11341 a        11569 c        10381 g        10787 t
ORIGIN      
        1 aaagatatag tttctcttcc agaagagaat catatttaaa agttgcgtac agatatcttt
       61 caatttcatg aatggcacgt tgtaactgaa aatctttttc aagaaactgc tcatagatat
      121 caccaatatt tttctgtttt attttttcat tacataacac acgactagaa aaatatttaa
      181 ggtgcatcat aaatctggag tagttaaatg aacttctgtc cacccttctg ttataaacaa
      241 cttcaataag ctccgtgatt ttttgagaca gctcaataat ccttactgat tcataatcaa
      301 ctgaattttc ataattattt aaaatatgca atgctataaa accaatctca tcatccggta
      361 gttgaacgct aaattgctca ttaatgattg ttaatgcatc acgacttatt gcatactctg
      421 ctttgtacaa cgctttgatt tcctcaagga aaacattatt aatttgtatg cctgagacga
      481 ctcgggatat tgcattcgaa atgtgatcag caagaatcag tagtagcgat ggattgagtt
      541 ttttttcgag attactctcc gcataatgca ctatcttttc ggcaacaaaa atatactcaa
      601 tacccacatg ctcaatcaat ttgtagagtt tatttttttg ctcatttctg acataaaaaa
      661 tccggtttgc aggatgctca gggaccgaca taccatattt tttattgtaa ccaacgccag
      721 gcccagaaat gataacctcc tgtccgttca ccgatgcctg tacacaatta ttgttcatga
      781 ctttttcgat gatcattcca tggctcccaa aaaaaaaggc aaggcatgtc agaagtaata
      841 attacttcaa acattgcctt gcctgattta acagttacaa gataattaac taatgcgtat
      901 accttacacg gaattgtgct taataaaaga gatgacaggc ttatttttta agccgatatt
      961 caaatattcg acagcagtca caaaaccatc gttcaacatt tatgtaaata atatttattc
     1021 aatgaaacat aaattaattg aataaattaa aaacatcatc cccttctcgc cagcgtcgcc
     1081 ccatcggcaa aatacgcttt aatccccgca agaatagact ccgcaacttc ctgctgaaag
     1141 gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt ttcgaccaga
     1201 atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac ctgattttta
     1261 tgcagcttgt tgattttacc gagcttattc agtaccgctt taccaaactt aaggctgtca
     1321 gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg gtcaccgctt
     1381 ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt gtgccagata ttttgccgca
     1441 gtactggttg cacctttggt tgagagcgca aacacagagg aaccgctcgg ctgacgactg
     1501 gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg ggcttttgct
     1561 acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac cttcatattg
     1621 ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac atctttttct
     1681 cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg gtcaagcata
     1741 atgacaatcg gacgatcgcg ccctgcttta cccggttgtg gaccactttg tgctggcggc
     1801 acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag cgggtcctgc
     1861 atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa cccggcgacc
     1921 ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac catccgtacg
     1981 gtctgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc tgcacgaatt
     2041 tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc caccaccacg
     2101 cgttccgggt tactcaacgc gaactgctta tatttcagct gacgatttga ttctaccgtc
     2161 acacgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct gaccgcagcc
     2221 agactgacct gacttacgct caatagccac atggcacccg cgccttgcag taaacgacga
     2281 cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa tctaagctgt
     2341 gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata atctgtcatc
     2401 taataaacgg taaacattct ttttatattc acggcattac tgataaaaaa gtcgctctcg
     2461 cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacacta atttcgaata
     2521 atcatgcaaa ggggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc gagggattcc
     2581 gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc atgctcggcg
     2641 gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg ttgttgcaca
     2701 gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg
     2761 ccgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac gccaaaacac
     2821 tggaactggt gaagcaggct gcgggaacat tgcaactgga catcactgct cgcctgtcga
     2881 tgagtctcaa taacacgccg ctacagggcg cgcatatcaa cgtcgtcagt ggcaatttta
     2941 ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc
     3001 ggcggattga tgaagacgcg atccatcgtc aactggacag cggtgcaata gtgctaatgg
     3061 ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa gagattgcca
     3121 ctcaactggc catcaaactg aaagctgaaa agatgattgg tttttgctct tcccaaggcg
     3181 tcactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg caagcacggg
     3241 tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt ttgcgtggcg
     3301 cagtgaaagc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat caggaagatg
     3361 gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa
     3421 gcgccgagca gattcgtcgc gcaacaatca acgatattgg cggtattctg gagttgattc
     3481 gcccgctgga gcagcaaggt attctggtgc gccgttctcg cgagcagctg gagatggaaa
     3541 tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc gcgctctatc
     3601 cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg gattaccgca
     3661 gttcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag cagagcggct
     3721 taagcaaatt atttgtgctg accacgcgca gtattcactg gttccaggaa cgtggattta
     3781 ccccagtgga tattgattta ctgcccgaga gcaaaaagca gttgtacaac taccagcgta
     3841 aatccaaagt gttgatggcg gatttggggt aagcgatgaa aatcgccgga tgcgacatgc
     3901 gtaacactcg tgcgtcgcat caggcaatta cgtttattcc cgcgaactaa acaatgccgc
     3961 cagaccactg cgccgctcag tgcgcgtggc gattgccgca cttaatatgc gctcatcggc
     4021 atacagcgac agacggcgac gcgcgcgggt cactgcggtg taaaccagct ctcgcgttac
     4081 taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg atccctgcga
     4141 tttatgtacc gtcatcgccc acgtcgtttc gtgctctggc aggcggctcg gttgcacaga
     4201 cttaatattg ccgtccggca tcgcaaacca aacgcgcgtc ccctgcccgc gatccagcgc
     4261 aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa tcatcaccgg
     4321 tcgaccttcg taccaacgag agtgcggatg acgatgaatt ttgcgcttct gttgcataaa
     4381 ctgctcaatt cgctcattca gtccagccac gccaaacggc ccttcccgca gggcgcacaa
     4441 aagttggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg cttgcagcag
     4501 atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat aatcttctcc
     4561 gctctgtaaa agtcgttttt cgatatcagt aaaatcctgc tgaaaaacgg ttttcactgc
     4621 cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat cgctgccgaa
     4681 acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt ctgtgccggt
     4741 tcctgccgga acgtgagtcc ccgtcaggcg gcttagctgc ctggcacgct cggcggtaaa
     4801 gcccgcgttg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa ccgaggccag
     4861 ttgatcacga tcgccgagaa agatcactcg cgcatgatct ggcaaggcgt cgatcagtct
     4921 cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat caagatgcag
     4981 cgggttacag gcatgatgac gtaaacgctg gctacccggc tgcgcgccca gcaatcggtg
     5041 caaagtgctg gcatcttccg gaatgcgttt cttttgttca tcggtcagcg gtaactgtcg
     5101 caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg gtgcagccag
     5161 acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca acttcgccac
     5221 ggtggtcgtt ttaccggtgc ctgggccgcc ggaaaccacc gagatccgcc gcgtcagcgc
     5281 cactgccgct gcaacttttt gccagttaat ttcatcgctt actggaaaaa gtttgtccag
     5341 ggtttgcgcc agtagagctt catcaacctc aatggcatga ttcacctcat tgaagaagcg
     5401 cgctaccgtc tgctcgttac accacatgcg attcaaataa agacgatcgc cacagaggac
     5461 catcggcgtg ggttcatctc ccctgctgac cgcttgagaa gccagcaagc attcttccca
     5521 attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggatgcg acgcctcgtt
     5581 attttccagt cgtgaaagcg gcaaacagac gtgtccctct ccggcatcat gacttaacag
     5641 tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg caaattgcac
     5701 atccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt gcaatttcat
     5761 tacgcctcct ccagggtcat accggcaaac atctcatcca tcagggcaat caacccggcg
     5821 ttgggtcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacgcc acgcaggaac
     5881 agatagataa cgccgccaaa gtgacgatca tagtcgtaat cagcaatgcg atggcgcaga
     5941 taacgatgca gcgccagggt ataaagctga tattgcagat catagcggtg tgcctgcatc
     6001 gctgctgcca tagcctgttg ggtgtaagcc gaactgtctt cacccagcca gttggattta
     6061 tagtcgagca ggtaataacg cccttcgtgg cggaatacca ggtcgataaa gccttttaac
     6121 atgcctcgta cctgcatgaa ctccagcggc gggcagccag cggatagcgg gtcaaactgg
     6181 cgaattagtg catcaagctg gctggcgatg agcggttcgc taatcggcag gtaaaactcc
     6241 atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt ttcattgaga
     6301 ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg cgattcaaag
     6361 ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt aaaatcgagg
     6421 tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa ctgatgcggt
     6481 gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc cagccgtggc
     6541 atcagatcct gagcgatacc gtgaccacgc tgctgcaagc cggagtagct ggtgacacgc
     6601 cagttatcgc cgggcaatcg ttgtagcatc ctcgcattca gttctgcagt taaagcatca
     6661 ttaacctgcc agggctggtt atcaccagtt tgtgccgttt gccaggcaat atcatcatcg
     6721 cataacgctt caatacaggt gcaaagccct gccgcatctt gcggttcccc tttttgcagc
     6781 aaacgcccga gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc acgacggcgc
     6841 accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc cacgtaaagc
     6901 aaacgcagat cttccgccag acgttcgacc tccgcaagct cgacgctttc tggcgcagcg
     6961 ttaagatcca gaacagcctc aaacgagtgg cgatcgtgat aaaacgcctg atcctggacg
     7021 cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt cgatttatgg
     7081 atcgtgacaa tctgcaccag atgtttatca ctttcgaggc gcatttgttg gctggaggca
     7141 ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg ttcactttcc
     7201 agctgcgttc cggcttcttg tagcagttcg ctgatatgca agatatcggt aagacgccgc
     7261 tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat cagcgcccgc
     7321 agcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa ctcttcaact
     7381 accgcatccc acgcatgttc gtcattattc agcgtttcga tatccagcgc gttcagcccc
     7441 atcattgacg ttgccagcgc actgcgcagg gtgttctcac gttcgggcgt catcaccgcc
     7501 tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc gcggttcgaa
     7561 aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc ggcctcctgg
     7621 cggctgcgca ccagcacact gatgtccgaa gcacgcaccg gacgcgcgtc gtcgccgttc
     7681 atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcggatttg cgcagcacat
     7741 acctgcgcca tggtactttg ataatcgcca acgccgcagc tttcgccttc catcagccac
     7801 attttcatcg caggctgcgt ttcaccttta aacacaaaac gtaacgcctg atttttcccg
     7861 gctgatttca ctggaataaa cggtatttcg cgaaacatga acgcgtcatc agtctggctg
     7921 aaaagcttgt tcacactgtt caccattcca ggtgcggagc gccagttggt gtctaaagtg
     7981 tagtgggcgt gaacttcact acgcgccttc atataagtga agatatccgc accccggaat
     8041 gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg ctgatggtgc
     8101 cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc atcgatcatt
     8161 gccaccggga atcgcgtacg gatcgccgct gccaacacct caccgctttc gctacgcagc
     8221 gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg gcgtttttca
     8281 cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaccagatc gcggatcgac
     8341 aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg ggtttccccc
     8401 ccggccttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga ctccggcaac
     8461 tgataactgt ttgtctcttc ttctgcccag gcgctgatct tttcgatcca tttagcctga
     8521 ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc atcaagttca
     8581 cccactgcgt cgcgccactg ctgttttacc gtatcaatac gcgccacaat ttgcgcgtgg
     8641 cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggtgc ttcgccttgc
     8701 agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc aaagaccacc
     8761 agggcaattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc gcaggcctgg
     8821 tagcgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatacc ggattcaaag
     8881 gcattcaggt tgagcatgcg ctggcaaaaa ccgtgaatag taaagactgc cgcttcatcc
     8941 atctgccgtt cggctaacaa caaccactgc gcggcttgcg ctttatcgtc gatctcttcc
     9001 agcaggcgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat gcgcaactcg
     9061 tggatattgc tacggatacg accacgcaat tctgccgtgg cagcctcggt aaaggtgacc
     9121 accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cggaaccgcc tagtccaagt
     9181 aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc agaggcttca
     9241 atcaggcgct cgccctgtaa gggcaagcgc aaaggatcta gtgtctcggc gacatcactc
     9301 attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct cccacacttt
     9361 ccagccttca ggatgcacat attcggcttt cccgttctgg ctgccggaaa tctgcgacag
     9421 aatagccatg ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa gtttttgcgg
     9481 cgtcagcagt tttatctggg ccacgatttt atcacgcgaa tcgaagcgca tattgccgcg
     9541 atcgaaatct ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg cctgcagcat
     9601 ctgggtaatt accgcctgct ggatttgcgc aaactcttct ggcttcatcg cccgcaattt
     9661 tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaaggaag gctgtttatc
     9721 attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg gaaatgcaaa
     9781 cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg gctgtacgat
     9841 ctgccccaac agagagctat aggctgagct ggtgtattca tcgtagccag tcggtacaaa
     9901 taccgctgcc agtgcggagt cggtgctgtt accagctttt tcaaagatga cggattgttt
     9961 tttatcgacc acgacatctt tgttacgaca ccactctgaa ccatcagcgc ccaactgttt
    10021 ttgcacatcg cgtgccagcg ttgttgcctg gtcctcggtc atgttgccga taaccataaa
    10081 ctctggtcga gcccctgatt ttaaggcgtc gcgataggcc agcacctctt tcaacgtaat
    10141 ggagggcaaa attttgcgcc gttcatctcg cgagaagtac ggcacttgcg agagcatctg
    10201 cgcgggcata atcgcctgct cgaacgcttt gcccttttct gcggaatcca tcatctggtt
    10261 ataccaggac ttcgcctgct caagctgatc ttccgtggcg gtatagctaa agtagccttc
    10321 cagcaatgcc tggaacagct gcggcagatg ctgggtgtaa ccattagcat taaccataag
    10381 gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctggt tgcttaactg
    10441 atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat tgcgggcgct
    10501 gtccatggct ttcggattac gcaaaatcag gctgacatcg gcttttggct cgctggcaaa
    10561 ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca gctctggatg
    10621 gtcatatttc ttgtctgact taatcagcga gaaatcgtca ggaatataag ggttaagctc
    10681 tggcaaagag agcgcaatgt cggcggcttt tttctgccag tcggcgaaag tttgtgcgct
    10741 gattttatcg acctgatacg gcgcatcgac aaagtaagcc gttttgttgt gcggctcttt
    10801 cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac gttcctttac
    10861 tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct caacaggaac
    10921 gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga ttgacggata
    10981 acggaagtcg atatccagca cattcgccac ttcatcgaag tattgtttat cgatcccttt
    11041 ttcacgtaac aaattgaggt agctaaaaat agccgccaca acctgatcgc gattcgccag
    11101 acctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt tgacgatagg
    11161 atcggagttg gcgctaatgc cctcaactaa tccctgcttt tgcagccagt cggaaagtgt
    11221 acctgggctg cgattaccaa tcagataggt aatcaattca tcggttttgc tacggaattt
    11281 cgccgagttg ttatcaatac gaaactctac gcgcaacact ttacgcggca gcgccgggac
    11341 gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga tttccggttt
    11401 tttgctctct ttgttcggca cgcgaccaaa ggtgtccgcc gccatttttg ccaactccgg
    11461 caacggttta ttgctgtaaa taaccgcctt catcagattg gcggaatagt acttctcgtg
    11521 gaaatctttc agcgcctgct gcaccggatt accaggtttg tcgcttaaag tttcgaggtt
    11581 accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc tgacctgtgc
    11641 catgcgcatc ccgtcacgcg tacgcgccat ggttaattca gcgttcactg cattacgctc
    11701 acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcatcagcca ggcggtctac
    11761 cgcaccaggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat acggcgcagt
    11821 gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt cagcctgcgg
    11881 gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc cctggtacgc
    11941 ctcgggatct tccagcgacc caacgggcac caccagcgcc gagagcgatt taactgcctg
    12001 cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct gatactggcg
    12061 gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg tttctgcctg
    12121 acttaagggt gcccaaaggg caactaacaa caataatgct ttgaaccagg tgctgcgggg
    12181 cattcacgga cctcataatc aacttaattt tctgtccaga ttcaacacgt taacgcaaat
    12241 catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat aaccatcagt
    12301 ccgtgactgg tgcgcatcat aaagtaagcg gatagattgc gcaattttta tacaacactc
    12361 atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca acgatggcct
    12421 ccattgtctc tggtgttaat tgccgccaga gcctttgata ccagatatca tcaccttcgc
    12481 cacgcaccat catgttgcct tcgtaagcct gaaggaattt cgtacgggct ttttgcaacg
    12541 tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt agccacgcgc
    12601 cgccactttc aggtaacacc agcaatggcg cggacatccc ttcacgatac ccctcaatca
    12661 gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc cactcgccgt
    12721 ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag acaaggtgtt
    12781 ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag cgcaacaggc
    12841 catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcag gcgagatcaa
    12901 tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc tgctgcatct
    12961 cctggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa tcccctgccg
    13021 cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtgca ttcaataact
    13081 gctgattgat ttgataacgg ctaagccctt ccagaataaa tggctcggtg tctgggattt
    13141 cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc cgcaccggat
    13201 gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta aacggcagcg
    13261 gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc cattcacgag
    13321 catagctttg tcgttcgcct ggctggtagt tttgcggatc aaacggcatc cgggtatgga
    13381 ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc gcttcatcgt
    13441 ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt accgacggga
    13501 aacgttcact gttatcctga atggaacgcc cgatatagct gatatagagt ttttgctgcg
    13561 cggaaattaa cgcttccagg aacaggtagc ggtcgtcatc gcgacggcta cggtcgccac
    13621 gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt ggataaacgc
    13681 cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt ggcatcagag
    13741 tacaaatgtt aaccggtccg gcgagaaaac gctggctgat acgttcttga tccagacgct
    13801 gcgccagttc atcacgcaat agtgacagcg gcaccgcgtc gccatactgc gcacctaaac
    13861 cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct tcggtttccg
    13921 catccggcag gaagaaggcg ttgagcatat cgcgacaaac cggcaaccac tcttctagcg
    13981 gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt gaagccagat
    14041 gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc gattgccact
    14101 cgccctgcgc gctctccatc gcgtagccca gcaacatacg cgtcaggcca aatcgccagg
    14161 tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct atcccccaac
    14221 gaatgccgga ttcgttgacc cactggcgta aataacgcag cccttcttcg gtgatgtcaa
    14281 accgcgccgc cagcaccggc acatccagta atgccagcac gtcttccgac acaaagcggc
    14341 tgtctggcaa cgataacagg ctgataaacg cttccagtac cggatgcgac tgccgcgccc
    14401 gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca aacacagcct
    14461 gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa
    14521 gtgtcgggtc ttcttccagc atcgccagca ggcgatcgtg caaaacttca acttcacgct
    14581 gcgggctatg gcaaacgtgg aaggtgatac tgctatccag tggatcaagc gggcgtttgt
    14641 tatcgctacg ggaaaactct tcgatgttca caccagcaac ggcacggttt tccagttcca
    14701 gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt gacatcgaca aaggcgtcca
    14761 gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca agcttacccc
    14821 atgaagccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag agctgcccgg
    14881 cattttcgct atcacgaaat agcggcaatt cgcgatcttc aacactgtgt cgacgctgac
    14941 gggtcagcag tttcgccaga taagcaggat ctttaatatc gccccagtaa taacggcagg
    15001 ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctggagc gcctggagat
    15061 aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtaac cccggcgggc
    15121 aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg cggtgccaac
    15181 gcggttgtcc gagttcatgg gtatattcca ccagcgcctt ccacaacggc gcttgccagg
    15241 cctgtgcttc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc agccactccg
    15301 ggcgatagac cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc tggaacagtt
    15361 ttcgcttgtc gctatcgtca gccagataat gccgcaacag ggtaaagtct tcgcgatcca
    15421 gcaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta aaggcgctct
    15481 ctttggggat ctccggtaac acccggacga acatatccca gataaagctc gctggcagcg
    15541 gaaaatcaat gtttgccgca ataccaaact tttgcgacag ggtcatttgc agccactgcg
    15601 ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc agccgttcgc
    15661 gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa tggtagaccc
    15721 ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc atctcgcctt
    15781 ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct
    15841 gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc
    15901 aaccatgccg ccagagctgt tggtactggt ttctgctggc gagactgttc attaatgttc
    15961 gctggatacc cgataacgca gtgacaatca tcaccatcag caccatcgca accattacct
    16021 ccggcaggct aaagccttgt tgattcttca gggaagctga cataacgccc tctctttcag
    16081 cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatg tttccatcaa tgacttcgcc
    16141 tgttcgccac aacgaaacgc cttcataacc ggcaaccaat aaggcttcat tatctgccag
    16201 taaacgcaaa caaacccggg catcggttcc agcgtactgc gagcactgaa ctgcgggctg
    16261 cgtctgccag gagtgcattt tcccccacgc cagcgccgac tgaacgatgg cctggcggcg
    16321 caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct gactcattcc
    16381 ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg acgaaacacc
    16441 tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac cacggtttgc
    16501 ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa gccgctgaca
    16561 tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt gactttatcc
    16621 cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg ctccttcaga
    16681 cgaaatccaa tctggtcaga ctcttttacc ggttcgcgat cccagatacc gttactgttc
    16741 gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc ttcgccggta
    16801 cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa tacccgcagc
    16861 cagatttcat cttccagcgc cagcttacgg gtgctcgtta aactttcacg ctgtaacgca
    16921 ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc aatcaacact
    16981 tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct tgctgacaaa
    17041 gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag tttttgaagc
    17101 gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa ggtgtcaggt
    17161 cgctcatttc gacttcgggc cagcgtggca caatgaccaa tggtgaactg ccatgacagg
    17221 tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg ataacgctga
    17281 tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa tagtcccgcg
    17341 cctggctggc ggtttgccat agccgttgcg actgctgcca gtattgccag ccatagagtc
    17401 cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc agcgtataac
    17461 cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga gggccagttt
    17521 ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg caaaatttcg
    17581 ggaaggcgtc tcgaagaatt taacggaggg taaaaaaaac cgacgcacac tggcgtcggc
    17641 tctggcagga tgtttcgtaa ttagatagcc accggcgctt taatgcccgg atgtggatcg
    17701 tagccttcaa tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg
    17761 ataatcaact tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata
    17821 tgattgctgt acaggtgcgt gtcgcccccc gtccagacaa aatcgcccac ttccagatca
    17881 cactgctgcg ccatcatatg caccagtaac gcgtagctgg caatgttaaa cggcaggccg
    17941 aggaaaacgt cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag
    18001 aactggaaga acgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac
    18061 gctgaaacaa taatgcggcg cgaatccggg tcgtttttca gctggttcag taccgtagtg
    18121 atctggtcaa tatgacgacc atccggcgtt ggccaggcgc gccactgttt accatacact
    18181 ggcccgaggt cgccgttttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt
    18241 agataagcaa tgttagtgtc gccctgcaga aaccacagca gttcatggat gatggaacgc
    18301 aggtggcaac gtttagttgt caccagcggg aatccatcct gcaggttaaa acgcatctga
    18361 tgaccaaaaa tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg
    18421 agcacttttt gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt
    18481 gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa
    18541 agaatttgtc ccatgctgat gtactgcacc caggcaccag taaactgcgc gtcgggctgg
    18601 cggaaaaact caacaatgat gcgaaacgcg ccgtaaccaa tcaggaacaa acctgagaca
    18661 gctcccattg ggcgcggttt acgaatatac aggttgagga taataaacag cactacacct
    18721 tccagcaaca gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtatcgaaa
    18781 atggattgcc actgcgggtt ggtttgcagc agcaaaatat cttctgtacg ggagccaggg
    18841 aacagcatgg caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag
    18901 ttgcccagac gcccggcacc aagaccaaac ggaatgagtg gtgcgataaa atcagagacc
    18961 tggaagaagg aacgtttagt acggcgggcg aagataatca tcaccacgat aacgccaatc
    19021 aggccgccgt ggaaggacat gccgccgtcc cagacacgga acagatacag cggatcggcc
    19081 ataaactgcg ggaagttgta gaacagaaca taaccaatac gtccaccgag gaagacgccg
    19141 aggaagcccg catagagtaa gttttcaact tcatttttgg tccagccact gcccggacga
    19201 ttcgcccgtc gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg
    19261 taccagtgaa gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc
    19321 agatagctac tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg
    19381 attgaaatgc gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga
    19441 cctggctggt tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg
    19501 cgaatcagcc cgcccatgcc gcgacgctcc ataaaggctg caacctgatg gcgaacttcg
    19561 gtcgccagtt gcgcttccag actacgctgc gcaagatttt ctgcttcggc ataatcaatg
    19621 cgccgcagca ggtattttac ccgcgctaca gaacgtccgt tcatagacag atggcgatac
    19681 ccaagcccaa tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacgg
    19741 agatcgattc catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca
    19801 ggatgaagac tgtcataaat gttcgccacc cgggtattgt tgcgatcaac ggccagaatg
    19861 tattgagtca gatcgttggt gccaacagag atgaaatcga cccgctttgc cagatgcggc
    19921 agcataaata ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg
    19981 taaccgatca tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcatca
    20041 acttcatcga ggcttgtgac catcggcaac agaatattca ggttgcccgt agcggcatta
    20101 gcacgcagca tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga
    20161 atcccacgcc aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta
    20221 tctgctccga catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc
    20281 ccctgatact gcgccacctg ttcttcttcc gacgggaaac cactttgcag catgaatggg
    20341 atttcagtgc gataaagacc gatgccatca atacggctgc ccagtttttc ttcatgttcc
    20401 gggcttaaac cagcattgag catgactttt atacgctcac cgctttttaa ctgcgcgggt
    20461 aaattgacgt catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga
    20521 tattcttgca gcagtaccgg ctccggatcg accagcaatt caccgcgata gccatcaacg
    20581 atcagcgtcc tacgatgcag caccgaaggc tgaatatccg cgcccatcac ggtagggatc
    20641 cccagcgcac gtaccatgat cgcagcatgg gagttggctg cgccatctcg cacgacaaca
    20701 ccgactaagc gatcctgggg cagctcagca agagttgtcg ctgacagttc atctgccacc
    20761 agaatgaaac gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc
    20821 aatcgctgac ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc
    20881 gcggcaaact gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag
    20941 cctttatcaa cctcggcaaa caattcgcga cgcagccggg tgtccgaaag taggtgcgag
    21001 taaagatcga aaatagccgc cgtttctttt tgtgctccgg cggcaaagcg tttgctgtag
    21061 cggcgaaact cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc
    21121 ggatccagcg ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag
    21181 ccttcggcaa tcgccacacc aggtgctgcc ggtaatgcgc ggattcgcgt ctggcgatat
    21241 tgcccaaaca gggcagtcaa ctgcgactgg gaaagaatag ctgccatctg ggtggcaagc
    21301 gtcaccagga aggattcttc actttcgtca tactggcgca actctcgttg ctgtaccacc
    21361 agtacaccaa gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt
    21421 tcttctttta cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt
    21481 atcggttccg ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca
    21541 gtgcgaccgc gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca
    21601 tgatcggcca ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc
    21661 agaatattta acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc
    21721 aggcgagtga gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgcgttt
    21781 tgtggttttg gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc
    21841 ctacggtaga catcacgttt aaatgacacc acctgtctga ccggatacca gtaacttacc
    21901 catcgccagc cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg
    21961 cttaccagct gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc
    22021 caacgcacca aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt
    22081 cgaacgtctt tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct
    22141 gcggattctc cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc
    22201 cgggcccaca ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag
    22261 ccatcgtcat caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc
    22321 atactccagg aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa
    22381 taactcacag ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc
    22441 accttttctg tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca
    22501 ttctgaataa gcccgatata gacattttaa aatattcatt attagtcaat gcattacatg
    22561 taatttgagt aatgaaaaag agtaattcgt gacccagctc acacctctca tttacgggtt
    22621 ggcgaaagat caaccaatgc cgtatttatc cacagaatgt gccactaagt taagcactga
    22681 accactaaaa actggagttt cgtcgcacgt caaggctgta aatggaaaca gtagtggagg
    22741 tttttcacag ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca
    22801 gtgaccagat ttggaaaacc tattgcagtg ttgcgcaact cgattaccgg cagcctaaaa
    22861 agcaatataa atcagagaat tgaacaacac atgtggaaaa agttacactg cgaatattcg
    22921 gcacataatt gctgtttgtt ttttaaccaa ggtatcatga catgtcccaa cctcgcccac
    22981 tgctctctcc tcccgaaact gaagaacagt tgttagcgca agcacagcaa ctttctggtt
    23041 atacattggg agaactggcg gcacttgccg ggctggttac gccagagaat ttaaaacgcg
    23101 ataagggctg gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac
    23161 ctgagcaaga ttttgctgct ctgggcgtgg aacttaaaac tatccctgtg gatagtcttg
    23221 gtcgtccgct ggaaacaaca ttcgtttgtg ttgccccgtt aacgggtaat agcggggtga
    23281 cctgggaaac cagccatgtg cgccacaagc tcaaacgcgt actgtggata ccggttgaag
    23341 gcgagcgcag catcccgctg gcgcagcgtc gcgtaggatc accgttgctg tggagcccga
    23401 atgaagagga agaccgacag ctacgcgaag actgggaaga attaatggat atgattgttc
    23461 tcggtcaggt tgagcggatc accgctcgtc acggggaata tttacagata agaccgaaag
    23521 cagcgaatgc gaaagcgctt accgaagcca ttggtgcccg gggcgaacgg attctgacgc
    23581 tgccacgcgg cttttatttg aagaagaatt tcaccagtgc actactggcc cgtcattttc
    23641 tgatccagta gccatcgctt tgacctgccg ctttccgggc atataattac cgcttcattt
    23701 ttttggcagg gctttttaga tgttatttgc atggataacc gatcctaacg cctggcttgc
    23761 gctcggtacg ctgacgctgc tggagatcgt tcttgggatc gacaatatta ttttcctttc
    23821 tctggtggtg gcaaagcttc ccacagcaca acgtgctcat gcgcgccgtc tggggttggc
    23881 gggagccatg gttatgcgtc tggcgctgct ggcatcaatc gcctgggtta cgcgcctgac
    23941 aaatccgctt tttacaatat tcagtcagga aatttctgcc cgtgatttga ttctgcttct
    24001 gggtggcttg ttccttatct ggaaagccag caaggaaatc cacgaatcca ttgaaggtga
    24061 agaagaaggg ctgaaaacac gcgtttcatc attcctcggc gctatcgtgc agattatgct
    24121 gctggatatt atctttagcc tcgactcggt gattacggct gtgggtctgt cagatcacct
    24181 gtttattatg atggcagccg tggtgattgc cgtaggcgtg atgatgttcg ccgcgcgctc
    24241 gattggtgat tttgtcgaac gccatccttc ggtaaaaatg ctggcgctct ctttcctgat
    24301 tctggtgggc tttaccctga ttctggaaag tttcgacatc cacgtaccga aaggttacat
    24361 ctacttcgcg atgttcttct ctattgcggt tgaaagcctc aacttgattc gcaacaaaaa
    24421 gaatccgctc tgatactccg tacgctctcc tgcgggaggg cgtactcact ccttttaccc
    24481 tcacagttta agattttcct gctttcaaga tatataacgt cggtttataa acagactatt
    24541 atcataggta ggccaaatga ggataaccgg atgaaaaaat gggcagtaat aatttctgca
    24601 gtcggactgg cgtttgctgt ttccgggtgt tccagtgatt acgtcatggc gaccaaagat
    24661 ggccgtatga ttttgaccga tggaaaacct gaaattgatg atgataccgg gctggtgagt
    24721 tatcacgatc agcaaggtaa cgcgatgcaa attaaccgtg atgatgtttc gcaaattatt
    24781 gaacgttaac aaataaggtc agcatccggc tggccttaag atttttctct ttcccttttc
    24841 ccttccctct gccattttta tattccttat gtcgtgatta taaaaaggaa acggctatgc
    24901 aatatcaccg tataccccac agttcgctgg aagtcagcac gctggggctt ggcacgatga
    24961 cgtttggtga acagaacagc gaagccgacg cccacgcaca actcgactat gccgtcgctc
    25021 agggcattaa ccttatcgac gttgccgaaa tgtacccagt acctccgcgc cccgaaacgc
    25081 aagggttaac cgaaacctac gtcggcaact ggctggcgaa acatggcagc cgcgaaaagt
    25141 taattatcgc ctccaaagtg agcggaccgt cgcgcaataa tgacaagggc atccgcccgg
    25201 atcaggcgct ggatcggaag aatatccgcg aagcgctgca tgacagcctc aagcgcctac
    25261 agactgatta cctcgatctt tatcaggtgc actggccgca gcgcccgacc aactgcttcg
    25321 gcaaactcgg ttatagctgg acggattctg cgcctgcggt ttcgctgctg gatacgctgg
    25381 acgcactggc agagtaccaa cgcgcgggaa aaattcgtta tatcggcgtg tcgaacgaaa
    25441 ctgcatttgg cgtaatgcgc tacctgcatc tggcggacaa acacgatctg ccgcgtattg
    25501 tcaccattca gaacccttac agtctgttaa accgcagttt tgaagtaggt ctggcagaag
    25561 tcagccagta tgaaggggtc gaactgctgg cctattcgtg cctgggtttc ggcacgctga
    25621 ccgggaaata tctcaatggt gcaaaacccg ctggcgcacg taatacgctc tttagtcggt
    25681 tcacccgcta tagcggtgag caaacgcaaa aagccgtcgc ggcgtatgtt gatatcgcca
    25741 gacgtcatgg cctggaccct gctcagatgg cgctcgcgtt tgtacgccgt caaccgtttg
    25801 ttgccagcac tctgctgggc gcaaccacga tggatcagct gaaaactaac atcgaaagtt
    25861 tgcatctgga gttaagcgaa gacgtattag ctgaaattga agcggtgcat caggtttata
    25921 cttatccggc accataagat taacgccggt taaaaccggc gttaaatatt aatgacggcg
    25981 ctgccagatc cacagcgccg ttattgccag cgcaaacagc gcaccgaagc caatgccaat
    26041 gggcacgacc gggatgccta tcattaccgc cagcgagtaa atgcccagca tcaacaacat
    26101 ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga cgcttttttt
    26161 accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca tcacgccaat
    26221 cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggaaaaaa tcagtaccac
    26281 cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg taactaactt
    26341 cgctgccgca cctgcgccaa ccacgatacc aatcgctacc atcgcgttga gataggtggg
    26401 cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca ggaaacgcag
    26461 cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg tttcaccatt
    26521 gcgccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga gattccagga
    26581 ctgccccgga cgcgccgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    26641 gtaggccagt gcgcatgcgg ccagggcgac gaggacatgc cagtcagcca gcacaccacc
    26701 ggctacggaa ccgagcaaaa tcgccgctat ggtagaagct tccattaaac cgttagcttt
    26761 cactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    26821 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    26881 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    26941 atccgccacc tgcccgacaa acggcgcaaa aagaatgtaa gcacctacaa acaccatttg
    27001 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    27061 cagtagggca ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    27121 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    27181 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    27241 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    27301 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    27361 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    27421 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    27481 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    27541 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    27601 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    27661 cttcgatacc aggaaccgac aacaggcgcg catccattcc tggtagaata cgccctaccg
    27721 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcac tcggttacac
    27781 catagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    27841 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    27901 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    27961 acaccaactc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    28021 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    28081 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    28141 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    28201 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    28261 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    28321 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    28381 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    28441 ttaccccagc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    28501 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    28561 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    28621 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    28681 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    28741 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    28801 tcgccacctg cgttggtggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    28861 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    28921 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    28981 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    29041 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    29101 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    29161 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    29221 ttagaacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    29281 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    29341 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    29401 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    29461 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    29521 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    29581 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    29641 tcatgtgctc aacccgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    29701 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    29761 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    29821 aaacacgccc ccccttgaac caacgggcgt tttccgtaac actgaaagaa tgtaagcgtt
    29881 tacctactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    29941 agtcgccacc gtttcccgcg tcattaataa ttcacctaaa gccagtgaag cttcccggct
    30001 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    30061 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    30121 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    30181 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    30241 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    30301 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    30361 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    30421 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    30481 tctgcaaggg tattacgatg cccttgctga aagtggtatt gcggccaatg accggctggt
    30541 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    30601 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gtgcgatggg
    30661 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    30721 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtgac
    30781 gatggcgacc caggctgccg aactggcttt ggcgctggcg gataatcgcc ctctcccgga
    30841 aatcactaat gtctttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    30901 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    30961 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    31021 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    31081 catgcagtac cagataatca cccgccttca cttccggcaa ggcgcgggtt tcaacatttc
    31141 ccccttcctg ctgggtaaag acatcgcccg attcacataa tggcccggcg acgacggttt
    31201 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    31261 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    31321 ggcggctacc catttgtttg acgctccgca cctgagtaat taacacgcca gactgcgcta
    31381 ccagaaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    31441 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    31501 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    31561 cgatgacctg acgtaccata gcaccacaca cctgttccag atgggcataa tcaacgccag
    31621 aaccaatgtg catgtgaatg ccgaccagct gcaaatgatg acgttgtatc acgtccagtg
    31681 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    31741 tttggctatg tccgtgacca aaccccgggt taacgcgcag ccatacccta tgccctggcg
    31801 aaacctggcc cagttggtcg agcatatcaa cagaacccgc attcaccgga atttgcaatt
    31861 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    31921 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    31981 aatccacttt cacgccctgc tcacgcatta atcgcaaaat atgaatattg gaacaggctt
    32041 tctgtgcaaa gcgcaccaca tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    32101 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    32161 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    32221 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    32281 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    32341 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    32401 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    32461 ggctaaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    32521 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    32581 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    32641 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    32701 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    32761 gactcactga gacactccat acgcctgcgg gaacagaacg taccgaatta ctctctttag
    32821 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    32881 atgattttca gggtgagaac tacatcagcc tttcccgtac tgacagctat cgccagttgc
    32941 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    33001 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtgt ttcggtggtt aacccgctca
    33061 ccgcactgga ttatgcggca agcggtttag tggtacggcg gttcagtatt gcggttccgt
    33121 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    33181 gtgagcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgcg attttgtcgt
    33241 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcag tatcaaacac
    33301 aggcaaaaca ctgcgctctt ctggaaccaa taaaccaatt tctgtgcagc caaaaatgac
    33361 accctgcgcc ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    33421 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    33481 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    33541 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    33601 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    33661 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    33721 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    33781 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag
    33841 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    33901 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    33961 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc cgatctctat
    34021 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    34081 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    34141 tccggaatga gccagaaagt gatgcccaca aacgcaatgt tcagcgcagt gtagagccag
    34201 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggtcgcgcc gataatcata
    34261 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    34321 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    34381 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    34441 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    34501 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    34561 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    34621 gccattttga agatacgcgg cgcatagtac atgatgatgt tcataccggt aaactgctgc
    34681 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    34741 agtgcccaac cgccttgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    34801 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    34861 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcaggact
    34921 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    34981 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    35041 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    35101 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    35161 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    35221 cgacctaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    35281 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    35341 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    35401 gctaccgaaa caaacatatt catacgccgc gtatcccgca aagaacgtgg cgttaaagca
    35461 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca tagtgaaaaa
    35521 atacgtgaac aactcacgca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    35581 tgcgacatgt cgttatgtga tggatattcc aattttcaaa ttaagttgaa ttattgagat
    35641 tattattaac cacctaattt tacagcagat aaaattcata aagttcatta attgataatt
    35701 aatatggatt atttcataac catgatatgg attatgatga tctacaggta taaaaaaccc
    35761 tgccatgcgg cagggtcata aaagtaagaa gaatgaatta acgcgccagc caaccgccat
    35821 ccacggcaat ggtataacca ttcacataat ctgaagcgct ggaggcaagg aacactatcg
    35881 gccccatcag gtcactcggc agtccccaac gaccagctgg aatgcggtcg agaatttccg
    35941 cgctacgttg ttcatctgcc cgtagttgtt gagtattgtt ggtcgccatg taacccggag
    36001 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt cgccatcaat cgcgtcacac
    36061 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    36121 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    36181 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtcct
    36241 tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    36301 caatatgacc aaactccgct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    36361 gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt
    36421 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    36481 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttcgagag
    36541 aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact agcgcaaatc
    36601 tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca tgccccagat
    36661 aaaggtataa gctttggttc cgacgccgga atgaatcgac cagctcgggg agatcaccgc
    36721 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    36781 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgagtgtg
    36841 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    36901 gcaggtttcc agcacatccg ggacaaaata tttgttaatt gtgcgacggt tactggtgag
    36961 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    37021 cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga gtggcggtat caatactggc
    37081 aaagacaact tcttttgcac ctttacccac atacagggcg tcgcggtgac cgatttcata
    37141 gcattggcca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    37201 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    37261 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    37321 gtactcatcg gcgacaaata cctcttcaac caaaaattca ttgcgcagcc cttgggtatc
    37381 cagcgttttt gcgtgcgcgc tgtggatgct ctgtcttacg tccacatcaa cctccgataa
    37441 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    37501 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt catttttaag
    37561 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    37621 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    37681 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    37741 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    37801 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    37861 cgatcgcgcc accattgaca ttgacattga cccgacgctc atcccactca agcatcttgc
    37921 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
    37981 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
    38041 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
    38101 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
    38161 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
    38221 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
    38281 tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
    38341 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
    38401 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
    38461 catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat
    38521 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
    38581 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagccca gaaccgcaaa
    38641 cgtcattaat agtgattgca gaaacgctat taggaagacc acctttaata gccgattgcc
    38701 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
    38761 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
    38821 cggcggaatg acccgctaac gcaccgcgaa agcagccgat aggtgtccgt aacgccccga
    38881 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
    38941 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
    39001 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
    39061 aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcca
    39121 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
    39181 actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct
    39241 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
    39301 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcgaa aacatcagca
    39361 ggtgaaggga taacgggcgc agtaacacac tactatggca agaagattgg taatctgatt
    39421 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
    39481 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
    39541 gtgagtctgg gtgttgtatt aatcctgaat ctcatttttc tgatgggacg ccatgcaacc
    39601 attcgggtaa tgggtttttt ggtattccca ttgattgcct atttcttatt tctttccatt
    39661 taccttgtcg gtagttggca acctgatcta ttaacgaccc aggtagagtt caatcagaat
    39721 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
    39781 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
    39841 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
    39901 tttagctgcc tgttttctat tccaccttcg tatattgaag cggctaaaga agaaggggtc
    39961 accattttat cggcgctttc tatgctgccg aacgccccag catggctgtc aatttccggg
    40021 attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt
    40081 gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
    40141 gcatttaacc gcgcactatc aattatgttg gtatcgctga tcaccttcat tgtttgttgc
    40201 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
    40261 cttttcatca tgcctacgtt gtcaacgtat ctgatcccgg cgcttaaacc ctggcgttcc
    40321 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
    40381 tgatatccat tgtgaatggt tcaaaattgc tacaattttg aaccattcca gttttcataa
    40441 tgttcattcg ttattattcc gcactatcac ttccctcatc atatcctagt attatcagtt
    40501 agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat gcataactta
    40561 gattcgcaag atatcttccg gtcattcaaa aacaatttgc gatatagata ttctcatacc
    40621 atgagatagt tatctgaacc accctcaacc aggtaagatt aaatcagtga aacataatca
    40681 tatttaataa cgagaaatgc attttaaaat gtcttaatca gaattatagc aaatacagat
    40741 taatcgcaat ttttcccagc aaaattacaa aatcatggtg tattgacatc atcaatcacc
    40801 aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt
    40861 tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca
    40921 ccgagtttgc agtactgcgc aaactcgtgt ttttgtatgt ggcccgaaga aagttcatat
    40981 tttaatcgtg gtgtggtaga aggtatctta acaaaaaacc acaatgcgag attaagcgga
    41041 tatatttttg tcgatttttc agtaagtttt ttacgtctat tccttgaaaa agactggatt
    41101 gactatcttg caagtactga catggggatt gttttagtca gcgaccgcaa tatgcaatcg
    41161 ttagctaatt actggcgaaa acacaactca gcaatatctg ctgtcatata taatgatgat
    41221 ggtcttgatg ttgccaatga aaagatcaga caactattta ttggtcgtta tttatcattt
    41281 accagaggta acacattaac tcagatggaa ttcaccataa tggggtatat ggtttctggt
    41341 tataatccat atcagattgc tgaagttctg gatatggata tccgtagcat ctacgcgtac
    41401 aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg aattatttat tcgttcacat
    41461 tcggtccaac attgatactc aacgaccagc cagaatcata ctctgtttat acgggcattt
    41521 attgaaatgt ccgtatttta taaataggcc gtcccctcaa gagtaaacac catagtattt
    41581 aaaaccatca cacgaaaaat tcagaagcat tacgaaatat gaatttacaa acaatagtgg
    41641 cataaatgtt aaccatgtta atttacgtaa agttttacgt tgcaacatta aagcctcatt
    41701 tcaatcatca tgataaatat aaaattaata tatatttatg ccgtaaatac caatatactt
    41761 agcaaactat gtgatctcca tttcgattga tttagtgttt attgacgtat gtactggatt
    41821 attaacgata atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc
    41881 gatcttaacg tcgttctgac aacgccagcc agtcgatgtt tatcacttct acttgaagct
    41941 tttcctgatg tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg
    42001 cgtgtgccta ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct
    42061 gttggtgata ctacccactc gctaattgca accgtgccga gaagaggatt caagatccat
    42121 aatgacatta acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat
    42181 gccccacctg ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat
    42241 ttcaataaca aaatcctcaa acatataaaa agtcatctaa ttatgttgag cgcatttgtc
    42301 ataggcgcat attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag
    42361 gattacaaga ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt
    42421 gacgggttga aagagttcga taaatataaa acacggatac tggattcggg gattaactgt
    42481 aaaaaacatc cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg
    42541 gcatgcaata aaaattataa ccaacatgaa gtggcagact gtttaaccct gtcttaccgt
    42601 gaggttaatc gtgattgatt ataaaaaaaa cttattattt attttagttt ttatcagtgg
    42661 gtttattctc ttcactgtct attcttacac tgcagaaaaa atgatatata acgaaacatg
    42721 cactgcaaat tgggtaattt ttaatgacca gggacgagca aatcttacca tcgactttat
    42781 gtataacaaa aaaaataaaa caggaactgt tgcgttgagt ggaacctggc aacaaggtaa
    42841 cagagaaagt aaatcaatac ggcgaaatat tgaatacaca tggattgaaa actatgacac
    42901 agcccattta acatcaaaga aagttaataa gtttgagatt atggatcaag ttgatgatga
    42961 tagacttgca caacttattc ctgattttta tgtctttcca gaaaaaagtg taagctataa
    43021 tatactaaag caaggtaagc atgcttttat tttgagcatt ggtaacagag caataatgca
    43081 ttgtgcaagg taaactaaaa tagataacaa aaagatggaa cctcgttaat cataaaacaa
    43141 taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt gatcaacatc
    43201 tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca tctggtgcaa
    43261 atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg tacagtcgat
    43321 gtaaacaata cagaaagagg attattaaga accagtttac tcagtgaatc aatagaggaa
    43381 aggactaacg tttctttaaa agaattgatt tcatcatctg ttaaactaaa ctcatcattg
    43441 acagatcgtg agatataact gcttttaact ttactcttta cgttgacttt attgacagaa
    43501 ttaacattca caaatcttga atttaatgcc catgatgttg tttgcgtgag aaccttctca
    43561 gtattaagga atttttttaa cggaatcctt ggtttctttt tcgagtccga atttacaata
    43621 tcatgcatat agaccatttc atgaatctgc gaaaactcaa tgtccattat atacctcaca
    43681 tttttaccgt gactcgatgt tactgttcaa taatcacctt ccatcaatac taaaattaat
    43741 acccctaatg tgccgataac aaatatagtc attctacgta acgtctccat aaggtgatat
    43801 ttgacattat cagaagctgc gaattcggat tttgctctaa tcaaatgaga gaaatgtaaa
    43861 atgaagccac gaaatattaa taatagccta ccactgcaac cattagttcc tgatcaggag
    43921 aacaaaaata agaaaaatga agagaaatcc gttaatccag ttaaaatcac aatggggtct
    43981 ggtttaaatt atattgaaca agaatctctt ggaggaaaat atctaacaca tgatttgtca
    44041 ataaagatag cggatatttc tgaagagata attcagca
//
LOCUS       DAZUFB010000031        43029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZUFB010000031 DAZUFB010000000
VERSION     DAZUFB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.031"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..1865)
                     /gene="ycaO"
                     /locus_tag="ACHY3M_001923"
     CDS             complement(105..1865)
                     /gene="ycaO"
                     /locus_tag="ACHY3M_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFS4316849.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1995..2687
                     /locus_tag="ACHY3M_001924"
     CDS             1995..2687
                     /locus_tag="ACHY3M_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4316850.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2886..3974
                     /gene="serC"
                     /locus_tag="ACHY3M_001925"
     CDS             2886..3974
                     /gene="serC"
                     /locus_tag="ACHY3M_001925"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFS4316851.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4045..5328
                     /gene="aroA"
                     /locus_tag="ACHY3M_001926"
     CDS             4045..5328
                     /gene="aroA"
                     /locus_tag="ACHY3M_001926"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4316852.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5497..6261
                     /gene="ycaL"
                     /locus_tag="ACHY3M_001927"
     CDS             5497..6261
                     /gene="ycaL"
                     /locus_tag="ACHY3M_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFS4316853.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6434..7117
                     /gene="cmk"
                     /locus_tag="ACHY3M_001928"
     CDS             6434..7117
                     /gene="cmk"
                     /locus_tag="ACHY3M_001928"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4316854.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7228..8901
                     /gene="rpsA"
                     /locus_tag="ACHY3M_001929"
     CDS             7228..8901
                     /gene="rpsA"
                     /locus_tag="ACHY3M_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4316855.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9061..9345
                     /gene="ihfB"
                     /locus_tag="ACHY3M_001930"
     CDS             9061..9345
                     /gene="ihfB"
                     /locus_tag="ACHY3M_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFS4316856.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9552..11816
                     /locus_tag="ACHY3M_001931"
     CDS             9552..11816
                     /locus_tag="ACHY3M_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFS4316857.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMHD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11853..13601
                     /gene="msbA"
                     /locus_tag="ACHY3M_001932"
     CDS             11853..13601
                     /gene="msbA"
                     /locus_tag="ACHY3M_001932"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFS4316858.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13598..14584
                     /gene="lpxK"
                     /locus_tag="ACHY3M_001933"
     CDS             13598..14584
                     /gene="lpxK"
                     /locus_tag="ACHY3M_001933"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFS4316859.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14621..15853
                     /gene="ycaQ"
                     /locus_tag="ACHY3M_001934"
     CDS             14621..15853
                     /gene="ycaQ"
                     /locus_tag="ACHY3M_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFS4316860.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15905..16087
                     /gene="ycaR"
                     /locus_tag="ACHY3M_001935"
     CDS             15905..16087
                     /gene="ycaR"
                     /locus_tag="ACHY3M_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFS4316861.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16084..16830
                     /gene="kdsB"
                     /locus_tag="ACHY3M_001936"
     CDS             16084..16830
                     /gene="kdsB"
                     /locus_tag="ACHY3M_001936"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS4316862.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16984..17877
                     /locus_tag="ACHY3M_001937"
     CDS             16984..17877
                     /locus_tag="ACHY3M_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFS4316863.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17854..18633)
                     /gene="elyC"
                     /locus_tag="ACHY3M_001938"
     CDS             complement(17854..18633)
                     /gene="elyC"
                     /locus_tag="ACHY3M_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFS4316864.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18769..19554
                     /gene="cmoM"
                     /locus_tag="ACHY3M_001939"
     CDS             18769..19554
                     /gene="cmoM"
                     /locus_tag="ACHY3M_001939"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFS4316865.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19551..20873
                     /gene="mukF"
                     /locus_tag="ACHY3M_001940"
     CDS             19551..20873
                     /gene="mukF"
                     /locus_tag="ACHY3M_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFS4316866.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20854..21558
                     /gene="mukE"
                     /locus_tag="ACHY3M_001941"
     CDS             20854..21558
                     /gene="mukE"
                     /locus_tag="ACHY3M_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFS4316867.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21558..26018
                     /gene="mukB"
                     /locus_tag="ACHY3M_001942"
     CDS             21558..26018
                     /gene="mukB"
                     /locus_tag="ACHY3M_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFS4316868.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQQQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTTLNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26279..28126
                     /gene="ldtD"
                     /locus_tag="ACHY3M_001943"
     CDS             26279..28126
                     /gene="ldtD"
                     /locus_tag="ACHY3M_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4316869.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYKNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28307..28855
                     /locus_tag="ACHY3M_001944"
     CDS             28307..28855
                     /locus_tag="ACHY3M_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFS4316870.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28882..29529
                     /gene="gloC"
                     /locus_tag="ACHY3M_001945"
     CDS             28882..29529
                     /gene="gloC"
                     /locus_tag="ACHY3M_001945"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFS4316871.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29751..30941)
                     /gene="aspC"
                     /locus_tag="ACHY3M_001946"
     CDS             complement(29751..30941)
                     /gene="aspC"
                     /locus_tag="ACHY3M_001946"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFS4316872.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31126..32214)
                     /gene="ompF"
                     /locus_tag="ACHY3M_001947"
     CDS             complement(31126..32214)
                     /gene="ompF"
                     /locus_tag="ACHY3M_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFS4316873.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(32816..34216)
                     /gene="asnS"
                     /locus_tag="ACHY3M_001948"
     CDS             complement(32816..34216)
                     /gene="asnS"
                     /locus_tag="ACHY3M_001948"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4316874.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(34385..35587)
                     /gene="pncB"
                     /locus_tag="ACHY3M_001949"
     CDS             complement(34385..35587)
                     /gene="pncB"
                     /locus_tag="ACHY3M_001949"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4316875.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            35853..38465
                     /gene="pepN"
                     /locus_tag="ACHY3M_001950"
     CDS             35853..38465
                     /gene="pepN"
                     /locus_tag="ACHY3M_001950"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFS4316876.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(38672..39439)
                     /gene="ssuB"
                     /locus_tag="ACHY3M_001951"
     CDS             complement(38672..39439)
                     /gene="ssuB"
                     /locus_tag="ACHY3M_001951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS4316877.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(39436..40227)
                     /gene="ssuC"
                     /locus_tag="ACHY3M_001952"
     CDS             complement(39436..40227)
                     /gene="ssuC"
                     /locus_tag="ACHY3M_001952"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFS4316878.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(40238..41383)
                     /gene="ssuD"
                     /locus_tag="ACHY3M_001953"
     CDS             complement(40238..41383)
                     /gene="ssuD"
                     /locus_tag="ACHY3M_001953"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFS4316879.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(41380..42339)
                     /gene="ssuA"
                     /locus_tag="ACHY3M_001954"
     CDS             complement(41380..42339)
                     /gene="ssuA"
                     /locus_tag="ACHY3M_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFS4316880.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(42332..42907)
                     /gene="ssuE"
                     /locus_tag="ACHY3M_001955"
     CDS             complement(42332..42907)
                     /gene="ssuE"
                     /locus_tag="ACHY3M_001955"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4316881.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT        10544 a        10696 c        11437 g        10352 t
ORIGIN      
        1 attattttat taatgcaaat atatgtaaag cggggcatta aaaaaacgcc ccgtaatata
       61 actcagacta atcattaagc ctacattgcg taggctattt gattttattt tgcccagaat
      121 gctgctttgg cgcgctgcag cttttcgtag gccttcaaca acgactgatg tgcagcaaac
      181 gcgtgcagat cgctatctac cggttgcagg ccgtaaaacg ccgcttcgcc gctcattgcc
      241 gcactggcgg cttctacggc atctgcgccg tacatgcgaa caaacgcatt cagatattgc
      301 agaggctggc gatcttcttc ctgtgccagt aataacaacg tttgcaggca gcgataatag
      361 ttggcgcgtt ccggactaaa tactgatgag ttaaactcca tcgtccattc ggtccagacc
      421 agagcctgtt ccagatcgcc accagccagc gccagcatgg cttttaattc accgatacgc
      481 agggtgtacc aaccgttatc cgacccggtc gccagaccca acagctcacg cacgcgggta
      541 aagtcatcaa aaccttcttc atccagttgc tcgatgaggt tcaggtaatc ttctttttcc
      601 cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat ggctgcccat actgttattc
      661 gcgagccaca gatcttcagc cggataaata tcggacatgc caggcacgat aatacggcaa
      721 gcataaacgc ccagatgctc gtaatcggca atataaactt ctttatcttc tttgttgaag
      781 atagccatca gcgtagcaaa ctcttcttcc gtggtgccgg agaaattcca gtccacaaac
      841 ggataatcgg catcctgctt gaacaggtcc caggagatta aaccgctgga atcgataaag
      901 tgcgtttcga ggttggtatg ttcagcgact tcttcatcat cgaaggttgg cggagtaaac
      961 acatccaaat ctttcaggcc acgaccttgc agcagctcgg tcacggtacg ttccagtgct
     1021 acgccaaaat caggatgcgc accgaaagag gcaaagcagg taccgttagc aggattgaac
     1081 agtaccacgc aaatcaccgg atactggccg ccaagcgaac cgtcataagc gaagattggg
     1141 aaaccctccg cttccagcgt ttcgatggct tcaactactg ctgggtaacg cgccagcacg
     1201 tctgccggga tctctggcag gctgatgctt tcagcaataa tgcggttttt cacgtagcgt
     1261 tcgaaaactt cagacaatcc ctgaacgcgt gcttcgttgc gggtattacc tgcggacata
     1321 ccgttggaga cgtacaggtt accaatgata ttcatcggaa tataaacggt ctgattgtcg
     1381 gactgacgcg taaacggcag gccgcaaata ccacgatctt cgttaccgga ttgtaggtca
     1441 atcagcatgc tgccggtcag ttcattctcc ggatcataaa acgcgcgcag acggtcatcg
     1501 agtagtcctt ctggcacatc gtcattttcg gtcagtggga accatttttc gttgggataa
     1561 tgcacgaacg gaccgttggc gatggtttcg cccagccaga aatcagcaaa aaagtagttg
     1621 gttgagagac gctcgaaata ttcaccaagt gcagaagcca gcgccgcttt tttggttgcg
     1681 ccttttccgt tggtaaaaca cagtgcgcac tctttgtcgc gaatatgtac agaccagacg
     1741 ttaggcacag gattcagcca ggaggcctct tcaatctgaa agccgaggtc tgaaagtttt
     1801 tgctggaagc gagcgatgga atcttccaga gcggcatctt tgccggggat aaatgtttgc
     1861 gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata atacgggttt tatctcaaag
     1921 gcgctatcac cgccgccatg ccgatgaact gttgactatg cttttagcgg ataaaccacg
     1981 ataagagcat aaaaatgaag gcgttcgatc tccaccgtat ggcatttgat aaagtgcctt
     2041 ttgatttcct tggcgaagtt gcactacgta gtctttatac ctttgtactg gtttttttgt
     2101 tcctcaaaat gaccggaaga cgcggtgtgc gacagatgtc gctgtttgaa gtgttaatca
     2161 ttctcacgct gggttcagcg gcgggagatg tggcgtttta tgatgatgtg ccgatggtgc
     2221 cggtacttat cgtctttatc actctggcgt tgttataccg cctggtaatg tggttgatgg
     2281 cgcacagtga aaaactggaa gatctactgg aaggtaagcc ggttgttatt attgaagatg
     2341 gcgagctggc ctggtcgaaa ctcaataact ccaacatgac ggaatttgag ttctttatgg
     2401 agctacgatt gcgtggcgtg gagcagctgg ggcaggtacg tctggcgatt ctcgaaacca
     2461 acgggcaaat cagtgtctat ttctttgaag atgacaaggt gaaaccgggt ttacttattt
     2521 tacccagtga ttgtacgcag cgttacaaag tggtgccgga gtcggcggac tatgcctgta
     2581 ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa acaattatgt ccgcgctgtg
     2641 caaatccaga atggacgaag gcaagtcggg caaaacgggt gacctgacag taaaaacatc
     2701 ggctttttgc taataatccg agagattctt ttgtgtgatg caagccacat ttttgccctc
     2761 aacggtttta ctcattgcga tgtgtgtcac tgaatgataa aaccgatagc cacaggaata
     2821 atgtattacc tgtggtcaca atcgattgac cgcgggttaa tagcaacgca acgtggtgag
     2881 gggaaatggc tcaaatcttc aattttagtt ctggtccggc aatgctaccg gcagaggtgc
     2941 ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct tggtacgtcg gtgatggaag
     3001 tgagtcaccg tggcaaagag ttcattcagg ttgcagagga agccgagaag gattttcgcg
     3061 atcttcttaa tgtcccctcc aactacaagg tattattctg ccatggcggt ggtcgcggtc
     3121 agtttgctgc ggtaccgctg aatattctcg gtgataaaac caccgcagat tatgttgatg
     3181 ccggttactg ggcggcaagt gccattaaag aagcgaaaaa atactgcacg cctaatgtct
     3241 ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa gccaatgcgt gaatggcaac
     3301 tctctgataa tgctgcttat atgcattact gcccgaatga aaccatcgac ggtatcgcca
     3361 tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc cgccgacttc tcttcaacca
     3421 ttctttcccg tccgattgac gtcagccgtt atggcgtgat ttacgctggc gcgcagaaaa
     3481 atatcggccc ggctggcctg acaatcgtca tcgttcgtga agatttgctg ggcaaagcga
     3541 atatcgcgtg tccgtcgatt ctggattatt ccatcctcaa cgataacggc tccatgttta
     3601 acacgccgcc gacatttgcc tggtatctat ctggtctggt ctttaaatgg ctgaaagcga
     3661 acggcggtgt agctgaaatg gataaaatca atcagcaaaa agcagaactg ctatatgggg
     3721 tgattgataa cagcgatttc taccgcaatg acgtggcgaa agctaaccgt tcgcggatga
     3781 acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt gttccttgaa gagtcttttg
     3841 ctgctggcct tcatgcactg aaaggtcacc gtgtggtcgg cggaatgcgc gcttctattt
     3901 ataacgccat gccgctggaa ggcgttaaag cgctgacaga cttcatggtt gagttcgaac
     3961 gccgtcacgg ttaatgccga aattttgctt aatccccaca gccagcctgt ggggttttta
     4021 tttctgttgt agagagttga gttcatggaa tccctgacgt tacaacccat cgctcgtgtc
     4081 gatggcacta ttaatctgcc cggttccaag agcgtttcta accgcgcttt attgctggcg
     4141 gcattagcac acggcaaaac agtattaacc aatctgctgg atagcgatga cgtgcgccat
     4201 atgctgaatg cattaacagc gttaggggta agctatacgc tttcagccga tcgtacgcgt
     4261 tgcgaaatta tcggtaacgg cggtccatta cacgcagaag gtgccctgga gttgttcctc
     4321 ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc tttgtctggg tagcaatgat
     4381 attgtgctga ccggtgagcc gcgtatgaaa gaacgcccga ttggtcatct ggtggatgcg
     4441 ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag aaaattatcc gccgttgcgt
     4501 ttacagggcg gctttactgg cggcaacgtt gacgttgatg gctccgtttc cagccaattc
     4561 ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag atacggtgat tcgtattaaa
     4621 ggcgatctgg tttctaaacc ttatatcgac atcacactca atctgatgaa gacgtttggt
     4681 gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa aaggcgggca gtcttatcag
     4741 tctccgggta cttatttggt cgaaggcgat gcatcttcgg cttcttactt tctggcagca
     4801 gcagcaatca aaggcggcac tgtaaaagtg accggtattg gacgtaacag tatgcagggt
     4861 gatattcgct ttgctgatgt gctggaaaaa atgggcgcga ccatttgctg gggcgatgat
     4921 tatatttcct gcacgcgtgg tgaactgaac gctattgata tggatatgaa ccatattccc
     4981 gatgcggcga tgaccattgc cacggcggcg ttatttgcaa aaggcaccac cacgctgcgc
     5041 aatatctata actggcgtgt taaagaaacc gatcgcctgt ttgcgatggc aacagaactg
     5101 cgtaaagtcg gtgcggaagt agaagagggg cacgattaca ttcgtatcac tccaccggaa
     5161 aaactgaact ttgccgagat cgcgacgtac aatgatcacc ggatggcgat gtgtttctcg
     5221 ctggtggcgt tgtcagatac accagtgacg attcttgatc ccaaatgtac ggccaaaaca
     5281 tttccggatt atttcgagca gttggcgcgg attagccagg cagcctgaat gaacaacggg
     5341 caataaatag ccaaatcttt ctttatcaaa acgtcggcac attgtcggcg ttttttttcg
     5401 gaccttgtga gtcattttga ttaatggtag cgtcgcttgt caatgtaagt tgttgataca
     5461 taatatttat atatgattaa tcaacggatg attcacatga agaatactaa attactgctg
     5521 gcgattgcga cctctgcagc attactgaca gggtgtcaaa atacccacgg tattgatacc
     5581 aatatggcta tcagctccgg tttaaatgcc tataaagcag caacattaag cgatgccgat
     5641 gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca gcggcaatca agtcgcaagt
     5701 aaatccagca agtacggtaa acgtctggca aaaatcgcca aagcattggg taacaatatt
     5761 aatggcacgc cggtcaacta taaggtttat atgaccagcg acgtcaacgc atgggcgatg
     5821 gcgaacggct gtgtccgtgt ctacagtggc ctgatggaca tgatgaatga caacgaaatt
     5881 gaaggtgttc tgggccatga actgggccac gtcgcgttgg gtcactcgct ggctgaaatg
     5941 aaagcttctt atgcgatcgt tgccgcacgc gatgccattt cagctaccag cggtgtggct
     6001 tcccagcttt cccgctcaca attgggtgat atcgcagaag gcgctattaa tgctaaatac
     6061 tcccgtgata aagagtccga agcagatgat ttctcctttg atctgttgaa gaaacgtggc
     6121 atcagcaccc aggggctggt tggcagcttt gaaaaactgg ctagtctgga tggcggtcgc
     6181 acccagtcca tgtttgactc tcacccacca tcaacagagc gtgcgcaaca catccgtgat
     6241 cgtatcgcct ctggtaagta aatcattgtc atctttcggg ctggtcttct gccagcccgc
     6301 tataattgcg caataaatcc ccatctgaat acagacaaaa ctggtttttg cacacaacgt
     6361 taacgatttg tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt acgcctgttt
     6421 taaggagata aagatgacgg caattgcccc ggttattacc attgatggcc caagcggtgc
     6481 agggaaaggc accttgtgta aggctatggc ggaagcattg caatggcatc tgctggattc
     6541 gggtgcaatt tatcgcgtac tggcattggc ggcattacat caccatgttg atgttgcgtc
     6601 agaagatgcg ctggtaccgc tggcatccca tctggatgta cgttttgtgt caaccaatgg
     6661 caatctggaa gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta ctcaggaagt
     6721 ggcgaatgca gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat tattgcgtcg
     6781 ccaacgcgcg tttcgcgaat tacccggcct gattgccgat ggccgcgaca tgggaacggt
     6841 ggtattccct gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag aacgtgcgca
     6901 tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt aactttgagc gccttttggc
     6961 cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg gtagcgccac tggttccggc
     7021 agccgatgct ttagtgttgg attccaccac cttaagcatt gagcaagtga ttgaaaaagc
     7081 gctacaatac gcgcgccaga aattggctct cgcataagcg accgaatttg cagtaccccc
     7141 gttgcaatgg aatgacagcg ggtatgttaa acaaccccat ccggcatgga gccaggtgga
     7201 cgttaaatat aaacctgaag attaaacatg actgaatctt ttgctcaact ctttgaagag
     7261 tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt tgttgctatc
     7321 gacaaagacg tagtactggt tgacgctggt ctgaaatctg agtccgccat cccggctgag
     7381 cagttcaaaa acgcccaggg cgagctggaa atccaggtag gtgatgaagt tgacgttgct
     7441 ctggacgcag tagaagacgg cttcggtgaa actctgctgt cccgtgagaa agctaaacgt
     7501 cacgaagcct ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt taccggtgtt
     7561 atcaacggca aagttaaggg cggcttcact gttgagctga acggtattcg tgcgttcctg
     7621 ccaggttctc tggtagacgt tcgtccggtg cgtgacactc tgcacctgga aggcaaagag
     7681 cttgaattta aagtaatcaa gctggatcag aagcgcaaca acgttgttgt ttctcgtcgt
     7741 gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa cctgcaggaa
     7801 ggcatggaag ttaaaggtat cgttaagaac ctcactgact acggtgcatt cgttgatctg
     7861 ggcggcgttg acggcctgct gcacatcact gacatggcct ggaaacgcgt taagcatccg
     7921 agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt cgaccgcgaa
     7981 cgtacccgtg tatccctggg cctgaaacag ttgggcgaag atccgtgggt agctatcgct
     8041 aaacgttatc cggaaggtac caaactgact ggtcgcgtga ccaacctgac cgactacggc
     8101 tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac acgtttccga aatggattgg
     8161 accaacaaaa acatccaccc gtccaaagtt gttaacgttg gcgatgtagt ggaagttatg
     8221 gttctggata tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca gtgcaaagct
     8281 aacccgtggc agcagttcgc ggaaacccac aacaagggcg accgtgttga aggtaaaatc
     8341 aagtctatca ctgacttcgg tatcttcatc ggcctggacg gcggcatcga cggcctggtt
     8401 cacctgtctg acatctcctg gaacgttgca ggcgaagaag cagttcgtga atacaaaaaa
     8461 ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg tatctccctg
     8521 ggcgttaaac agctcgcaga agatccgttc aacaactggg ttgctctgaa caagaaaggc
     8581 gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt agaactggct
     8641 gacggcgttg aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt tgaagacgct
     8701 accctggttc tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt tgatcgtaaa
     8761 aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa agatgcaatc
     8821 gcaactgtta acaaacagga agatgcaaac ttctccaaca acgcaatggc tgaagctttc
     8881 aaagcagcta aaggcgagta attctctgac tcttcgggat ttttattccg aagtttgttg
     8941 agtttacttg acagattgca ggtttcgtcc tgtaatcaag cactaagggc ggctacggcc
     9001 gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc tttaaggaac cggaggaatc
     9061 atgaccaagt cagaattgat agaaagactt gccacccagc aatcgcacat tcccgccaag
     9121 acggttgaag atgcagtaaa agagatgctg gagcatatgg cctcgactct tgcgcagggc
     9181 gagcgtattg aaatccgcgg tttcggcagt ttctctttgc actaccgcgc accacgtacc
     9241 ggacgtaatc cgaagactgg cgataaagta gaactggaag gaaaatacgt tcctcacttt
     9301 aaacctggta aagaactgcg cgatcgcgcc aatatttacg gttaagtttt tactcaaact
     9361 tgaacgcgag aaaagcacct gtcgggtgct tttttcattt ctctaatcag gaactggaag
     9421 ctgcctcgca gagttttgag cagttttcac cctttcgtta aattcttctg aatgtgcctc
     9481 ggggaacgca aaattcccac cagacaaccg ctcaacaaag ttgcacactt tccataaaca
     9541 gggaggggtg catgaaaata acgacagtcg gcgtatgcat aatttgcgga atttttccgt
     9601 tgctgatttt gccccaattg cctgggacat taacccttgc gtttctgact ctcttcgcct
     9661 gcgtactggc atttatccct gttaaaaccg tccgttatat cgcgctgacg ttgctgtttt
     9721 tcgtttgggg catattatca gcaaagcaaa ttttgtgggc aggagaaacc ttaactggcg
     9781 cgacgcagga tgcaattgtt gagatcactg caactgacgg catgaccact cattacggtc
     9841 aaattactca tctacaaggt cgacgtatat tccctgcgcc aggccttgtg ctgtatggcg
     9901 aatatcttcc gcaagcggtt tgtgctggac aacaatggtc aatgaaactc aaagttcgtg
     9961 cagttcatgg ccaacttaat gatggcggct ttgatagcca gcgttatgcc attgcccagc
    10021 atcagccgct caccggccgc tttctgcagg caagtgtcat tgaaccgaat tgtagcctgc
    10081 gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc ctatatgtgg aatgcggtta
    10141 ttcttggttt aggtatgggg gaacggttat ccgtccccaa agaaatcaaa aatatcatgc
    10201 atgatactgg aacggcgcat ttaatggcga tatcgggatt gcacatcgct tttgcggcgt
    10261 tgctggctgc cggactcatt cgcggtgggc aagtttatct gcctgggcgc tggatccact
    10321 ggcaaatgcc attaattggt ggaatctgct gtgctgcttt ttatgcctgg ttgacgggaa
    10381 tgcaacctcc tgcattgcgt accgtggtgg cgcttgctac gtggggaatg cttaagttaa
    10441 gtgggcgaca gtggagtggc tgggatgtat ggatatgttg tctggcggca attttgctga
    10501 tggatcctgt tgccattctc tcgcaaagtt tatggctctc tgccgctgcg gtcgcggcac
    10561 tgattttttg gtaccagtgg tttccctgtc ctgagtggca actgccgccg gtattgcgtg
    10621 cagttgtttc cctcattcat ttgcaactgg gaatcacact cctgcttatg cctgtgcaaa
    10681 tcgtcatatt tcatggcatt agtctgacct cgtttattgc aaatctatta gcaattccct
    10741 tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat ggttgtgcat ttaagcgggc
    10801 cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg gtctttggct ttacttttct
    10861 gggggttaaa gagtttgcca gaagggtgga tcaacattgc tgaacgttgg caatggctat
    10921 cattttcccc atggttctta ctggtggtat ggcgattaaa tgcctggcga acgttgccag
    10981 caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct gtggcaaaaa cctcgacctg
    11041 acgagtggca ggtgtacatg cttgatgtcg ggcaagggct ggcaatggtg atagccagaa
    11101 acggcaaagc gattctctat gacacaggac tggcctggcc cgaaggggat agtgggcaac
    11161 aactgattat cccctggctc cactggcata atcttgaacc ggaaggcgtt attctgagtc
    11221 atgaacatct ggatcaccgg ggagggctgg actcaatatt gcacacatgg ccgatgttat
    11281 ggatcagaag tccgttaaac tgggaacatc atcagccctg tgtgcgtggc gaagcgtggc
    11341 aatggcaagg attgcgtttc agcgtgcact ggcctttaca agctagcaac gataaaggaa
    11401 ataaccattc ctgtgtggtt aaggttgatg acgggacgaa tagcattctt ctaaccggtg
    11461 atattgaagt ccccgctgaa caaaagatgc taagccgtta ctggcagcaa gtgcagacaa
    11521 cattgcttca ggtacctcac catggcagta atacctcatc atcgttgcca ttaattcagc
    11581 gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta taacgcatgg cgattgccct
    11641 ctaataaagt taagcatcgc tatcaacagc aaggatatca atggcttgat actccacatc
    11701 agggtcaagt gacggtcaat ttttcagcgc aaggctggcg gattagcagc ctcagagagc
    11761 aaattttacc tcgttggtat catcagtggt ttggcgtgcc agtggataac gggtagaata
    11821 tgcggctatt tcaacaaatg ctggtttttt gaatgcataa cgacaaagat ctctctacgt
    11881 ggcagacgtt ccgccgactg tggccaacca ttgcgccttt taaagcgggt ctgatcgtgg
    11941 cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc
    12001 cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg
    12061 tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct
    12121 cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg
    12181 gaatgccggt ttcatttttt gacaaacagt caacggggac gctgttgtca cgtatcacct
    12241 acgattccga acaggttgct tcttcttctt ccggcgcact gataacggtt gtgcgtgaag
    12301 gtgcgtcaat catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca
    12361 ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgtagtatcg aagcgttttc
    12421 gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc gccgaacaaa
    12481 tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct
    12541 ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca
    12601 tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg
    12661 cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt
    12721 caatgattgc actgatgcgt ccgctgaaat cactgaccaa cgttaacgcc cagttccagc
    12781 gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagacg
    12841 aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta
    12901 cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga
    12961 agacggttgc tctggttgga cgctctggtt caggtaaatc aaccatcgcc agcctgatca
    13021 ctcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt
    13081 ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta
    13141 acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa
    13201 ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc
    13261 tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg
    13321 ctatcgctcg agccttgctg cgtgatagcc cgattctgat tctggatgaa gctacctcgg
    13381 ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc
    13441 gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg
    13501 tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg
    13561 gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg atcgaaaaaa tctggtctgg
    13621 tgaatcccct ttgtggcggc tattgctgcc actctcctgg ttgtatggcc tggtaagtgg
    13681 cgcgatccgt ctttgctata aactaaaact gaagcgcgcc tggcgtgccc ccgtaccggt
    13741 tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa accccggtcg ttgtctggct
    13801 ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc gtatcgcggg gatatggtgg
    13861 taaggctgaa tcttatccgc tgttattgtc ggcagatacc acaacagcac aggcgggtga
    13921 tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt gcggtttctc ccgttcgttc
    13981 tgatgcggta aaagccattc tggcgcaaca ccctgatgtg cagatcatcg taaccgacga
    14041 cggtttacag cattaccgtc tggcgcgtga tgtggaaatt gtcgttattg atggtgtgcg
    14101 tcgctttggc aatggctggt ggttgccggc ggggccaatg cgtgagcgag cggggcgctt
    14161 aaagtcggtt gatgcggtaa tcgtcaacgg cggtgtccct cgcagcggtg aaatccccat
    14221 gcatctgctg ccgggtcagg cggtgaattt acgtaccggt acgcgttgtg acgttgctca
    14281 gcttgaacat gtagtggcga tggcggggat tgggcatccg ccgcgctttt ttgccacgct
    14341 gaagatgtgt ggcgtacaac cggaaaaatg tgtaccgctg gccgatcatc agtctttgaa
    14401 ccatgcggat gtcagtgcgt tggtaagcgc cgggcaaacg ctggtaatga ctgaaaaaga
    14461 tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg tatttgcctg tagacgcaca
    14521 gctttcaggt gatgaaccag cgaaactgct tacgcaacta acctcgctgg cttctggcaa
    14581 ctagttacgc cgcggcagcg ttcgattgat ggagtcatga atgtcgctgc cgcacctctc
    14641 ccttgctgat gcgcgtaatc ttcaccttgc cgcacaaggc ctgttaaaca aaccccgccg
    14701 tcaagcgtcg ttggaggata ttccggcaac gatctcccgc atgtccttgc tgcaaatcga
    14761 taccatcaat attgttgccc gtagtccata tctggtgctt ttcagtcgtc tgggaaatta
    14821 tcctgcccag tggctggatg agtctctggc gcgtggcgaa ttaatggaat actgggcgca
    14881 tgaagcctgc tttatgccgc gtagcgactt tcgtcttatt cgccaccgca tgctggcacc
    14941 tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag gaacatgagg cggaaattgc
    15001 acagttaatt cagcatattc atgataaggg gccggtacgt tcagccgatt ttgagcatcc
    15061 tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat aaacggcatc tggaaggttt
    15121 atttactgcc ggaaaggtga tggtgattga acggcgcaac ttccagcgcg tttatgattt
    15181 aacccaccgt gtcatgcctg actgggatga tgagcgcgat ctcgtttcgc aaacagaagc
    15241 agaaatcatc atgctggata acagtgcgcg tagcctggga atattccgcg aacagtggct
    15301 ggcagattac tatcggctga aacgtccggc actggcggcg tggcgcgaag cgagggctga
    15361 acagcagcaa atcattgctg tgcatgttga aaaattgggc aatctttggc tgcatgctga
    15421 tttgctgccg ctactcgagc gtgcgctggc cggaaagctc actgcaacgc acagcgcggt
    15481 actttcgcct tttgatcctg ttgtctggga tcgcaaacgc gcagagcagc tttttgattt
    15541 tagctaccgg ctggagtgct ataccccagc gccgaaacgc cagtatggct attttgttct
    15601 gccattatta catcgtgggc aattagttgg gcgaatggat gccaaaatgc atcgccagac
    15661 aggcatcctt gaagttatct ctctgtggtt acaggaaggc attaaaccaa cgacaatgct
    15721 gcaaaaaggg ttacgtcagg cgattactga tttcgcgagc tggcagcagg caacgcgggt
    15781 gacattagga cgctgcccgc aaggtctctt tactgattgc cgcactggct gggaaataga
    15841 ccccgtcgca taaatgatta tgataagatt tatgcattca taacccgatc catctggagg
    15901 aactatggat catcgtttgc ttgaaatcat tgcctgcccg gtttgcaacg gaaaactttg
    15961 gtataaccag gaaaaacaag agcttatttg caaactggat aacctggctt tcccgctccg
    16021 cgatggcatt ccagtgttgc tggaaacgga agcccgcgtg ctgactgctg atgagagtaa
    16081 atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt ccacgcgtct gcccggtaaa
    16141 ccattggttg atattaacgg caaacccatg attgttcatg ttcttgaacg cgcgcgtgaa
    16201 tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg atgttgcccg cgccgttgaa
    16261 gccgctggcg gtgaagtatg tatgacgcgc gccgatcatc agtcaggaac agaacgtctg
    16321 gcggaagttg tcgaaaaatg cgcattcagc gacgacacgg tgatcgttaa tgtgcagggt
    16381 gatgaaccga tgatccctgc gacaatcatt cgtcaggttg ctgataacct cgctcagcgt
    16441 caggtgggta tggcgactct ggcggtgcca atccacaatg cggaagaagc gtttaacccg
    16501 aatgcggtga aagtggttct cgacgctgaa gggtatgcac tgtacttctc acgcgccacc
    16561 attccttggg atcgtgatcg ttttgcagaa ggccttgaaa ccgttggcga taacttcctg
    16621 cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc gtcgttacgt caactggcag
    16681 gcaagtccgt tagaacacat cgaaatgtta gagcagcttc gtgttctgtg gtacggcgaa
    16741 aaaatccatg ttgctgttgc tcaggaagtt cctggcacag gtgtggatac ccctgaagat
    16801 cttgagcgcg ttcgcgctga aatgcgctaa tttcacttca cgacacttca gccaattttg
    16861 ggaggagtgt cgtaccgtta cgattttcct caatttttct tttcaacaat tgatctcatt
    16921 caggtgacat cttttatatt ggcgctcatt atgaaagcag tagcttttat gagggtaatc
    16981 tgaatggaac agctgcgtgc cgaattaagc catttactgg gcgaaaaact cagtcgtatt
    17041 gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt tgtatgacag ccagggaaac
    17101 ccaatgccgt taatggcaag aagctttagt acgcccggaa aagcccgaca actggcatgg
    17161 aaaaccacca tgctggcaag aagtgggact gtccgtatgc cgactattta tggtgtgatg
    17221 acgcatgaag aacaccccgg ccctgatgtc ctgctactgg agcggatgcg tggtgtttcg
    17281 gtggaggcac cagcccgaac accagaacgc tgggaacagc tcaaagacca aatcgttgaa
    17341 gccttactgg cctggcaccg tcaggacagt cgcggttgcg tcggcgcggt cgacaatact
    17401 caggaaaatt tctggccctc atggtacctg caacatgttg aagtgctatg gaccacgctc
    17461 aatcagttca ataacaccgg tctgacgatg caggataagc ggatcctgtt tcgcactcgc
    17521 gaatgtctcc cggcattatt tgaaggcttt aacgacaatt gtgtgttgat tcacggtaac
    17581 ttctgtttac gcagcatgct gaaagattcg cgcagcgatc agttactggc gatggtcggg
    17641 ccgggactaa tgctttgggc accgcgagaa tacgaactgt tccgactaat ggataattct
    17701 ctggcggaag atttgctctg gagttacctg caacgcgcgc cagtggcgga gtcgttcatc
    17761 tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc aactggttaa taccggacga
    17821 tttagtcggc gcaacttcga tctggcatca aaatcactct tgccgtggct cgcctgacgg
    17881 acctttcagc cactgccaga tgcggccaag cgtttcatag ccaacacgat cgctatgcat
    17941 cagccagacc ggagaaggga ttgcccgttc ccacgggtta agcggcgagt cgatggccaa
    18001 ctgattcgcc ggggctggta gcggatttaa cccttcctgc tgaaaaaaga tcatcgcccg
    18061 cggcagatgt gatgcggagg tcaccagcag gaaaggggcg tcaccaatcg cctgtttcac
    18121 tgccgcagct tcttcttcgg tatcttttgg caaatccagg gtgataattt gctcgcgcgg
    18181 cacgcccagc gattgcgcaa ctctggctcc tacttccgct gtactcaccg tattggtttt
    18241 tgctacgcct cccgtgaaga tcagttttga tcccggattt tcgcgccata agcgaatacc
    18301 ttcattcagg cgtggcagac tgttattgat taaattagag ctcggtgccc actgcgggtt
    18361 ccaggtataa ccacctccga gcaccacgat atagtccact ttttgcgaat tattccatgt
    18421 cggataggtg ctttcgatgg gacgcagcag gcggtcggct accggttgca aactcaataa
    18481 caagagtgcc agccacccaa tgctaataaa aatcttcccg gttttttgaa aacggctaaa
    18541 ccacagcagc gccaggccag cgccaataat taacaacatc agcggaagtg gcagcaacat
    18601 attgccaatc acttttttca gtgtaaaaag cattctttct ggttcctttt ttaaccatat
    18661 agcaagggat cgccagcgat attacaccag acgggttcat tctcgccgtg gctgtgacaa
    18721 aatagcgtct ttatcagaaa acgtcttagc caaccggtgg agaaagcaat gcaggatcgc
    18781 aattttgatg atattgcgga aaagttttcc cgtaacattt acggcaccac caaagggcag
    18841 cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc tggcggaaat gggcccgcaa
    18901 aaactgcgtg tgctggatgc tggcggtgga gaagggcaga ccgcaatcaa aatggccgag
    18961 cgtgggcatc aggtcatttt atgcgatctt tctgcgcaga tgatcgaccg cgcaaaacag
    19021 gcggcagaag caaaaggtgt gagcgacaac atgcaattta tacattgcgc cgctcaggat
    19081 gttgcttcgc atttggaaac gcccgttgat ctgatattgt tccatgcggt gctcgagtgg
    19141 gtggccgatc cccgcagtgt attgcagacc ctgtggtcag tgttgcgtcc aggcggcgtg
    19201 ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc ataacatggt cgccgggaat
    19261 tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac ggacgctttc gccagattat
    19321 ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag ctggttggca aattatgggt
    19381 aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga agcaccagca gcgcgactgc
    19441 tatgaagcat tacttgaatt agaaacgcgt tattgccgtc aggaaccgta tattaccctg
    19501 gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca aggataaagt atgagtgaat
    19561 tttcccagac agtccccgaa ctggttgcct gggccagaaa aaatgacttc tccatctcgc
    19621 tgccggtaga ccgactctct tttctgctgg cggttgccac gctgaacggc gagcgtctgg
    19681 atggtgagat gagtgaaggc gagctggtgg atgcattccg ccatgtgagt gatgcgtttg
    19741 agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc gatcaacgac atggtgcgtc
    19801 aacgtctgct gaaccgcttt accagcgagc aggcggaagg gaacgcaatt taccgtctga
    19861 cgccgctcgg catcggcatt actgactact acatccgtca gcgcgagttt tctacgctgc
    19921 gtctttctat gcagttgtcg attgtggcgg gtgagctcaa acgcgcagca gatgccgccg
    19981 aagagggcgg tgatgaattt cactggcacc gtaatgtcta tgcgccactg aaatattcgg
    20041 tagcagaaat tttcgacagt atcgacctga cgcaacgtct gatggacgaa cagcagcagc
    20101 aggtgaagga cgatatcgcc cagttgctga acaaagactg gcgggcggcg atttccagct
    20161 gtgaattgtt gctttcggaa acttccggga cgctgcgtga attgcaggat acgctggaag
    20221 cggcaggcga caaattgcag gctaatctgt tgcgcattca ggatgcgacg atgacccatg
    20281 acgatctgca tttcgtcgat cgtctggtgt tcgatctgca gagcaaactc gatcgtatta
    20341 tcagttgggg ccagcaatcc atcgacttgt ggattggcta cgaccgccac gtacacaaat
    20401 ttattcgtac cgcgatcgat atggataaaa accgcgtctt tgctcagcgg ttacgtcagt
    20461 cggtacaaac ctattttgat gagccgtggg cgctaactta tgccaatgcc gatcgtctgc
    20521 tggatatgcg tgacgaagag atggcactgc gcgatgaaga agtgactggg gaacttcctg
    20581 aggatctgga atacgaagag tttaacgaga tccgcgaaca gctggcggcg atcatcgaag
    20641 aacaacttgc cgtgtacaaa accagacaag tgccgctgga tcttggtctg gtggtacgcg
    20701 aatatctgtc acagtatccg cgtgcacgtc actttgacgt tgcgcgtatt gttattgatc
    20761 aggcggtacg tcttggcgta gcgcaagcag atttcaccgg actgccagcg aaatggcagc
    20821 cgattaatga ttacggagcc aaggtacagg cgcatgtcat cgacaaatat tgaacaagtg
    20881 atgccggtta agctggcgca ggcgctggcg aatccgttat ttccggcgct ggacagcgcc
    20941 ttacgttcag gccgccatat tggcctcgac gaactggata atcatgcatt cctgatggat
    21001 tttcaggaat atctggaaga gttttacgcg cgttataacg ttgagcttat tcgcgcacca
    21061 gaagggttct tctatttacg cccacgttcc accacgctga tccctcgttc cgtcttgtcg
    21121 gaactggata tgatggtcgg gaaaatcctc tgttatctct atctcagccc ggaacggctg
    21181 gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg aactgctcac cctggccgat
    21241 gaagcaaaac tgctgaaact ggtgaacaac cgttcaaccg gttcagacgt tgaccgtcag
    21301 aagttgcagg agaaagtacg ttcttcgctc aaccgtctgc gtcgtttagg catggtgtgg
    21361 tttatgggcc acgacagcag caagtttcgc attaccgaat cggtgttccg cttcggagcc
    21421 gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc gcctgattcg tgatggcgaa
    21481 gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa ccgaagaaaa tcagccagat
    21541 agcggagagg aagaataatg attgaacgcg gtaaatttcg ctcactgacg ctgattaact
    21601 ggaacggctt ttttgcccga acttttgacc ttgacgagct ggtcacgacg ctttctggcg
    21661 gtaacggggc gggtaaatcc accaccatgg cggcgttcgt tacggcgctg atccccgacc
    21721 tgaccctgct gcatttccgt aacactacgg aagccggggc caccagcggt tcgcgcgata
    21781 aaggtctgca cggtaagctg aaagcgggtg tctgttattc gatgctcgac accattaact
    21841 cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca ggttgccgga cgcgatcgta
    21901 aagtggatat caaaccattt gccattcagg gactgccgat gtcggtgcag ccgacacagc
    21961 tggtgaccga aaccctgaac gaacgccagg cgcgcgtgct gccgcttaac gagctgaaag
    22021 acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt caactccatt accgattacc
    22081 actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct gcgctccgca tctgaccgta
    22141 gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg gatctccagt gccattaccc
    22201 gttctctgcg cgactacctg ttgccggaaa acagcggcgt gcgtaaagcg ttccaggaca
    22261 tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc gattcgtgtc acccagtcgg
    22321 accgtgacct gtttaagcat ctgatcagcg aagctaccaa ctacgtggcg gcggactaca
    22381 tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc cctggagttt cgtcgcgagt
    22441 tacatacttc gcgtcagcaa ctggcggctg agcagtacaa acacgtcgat atggcgcgtg
    22501 agctggcaga gcacaacggt gccgaaggcg atctggaagc ggattatcag gcggccagcg
    22561 atcacctgaa cctggtgcaa accgcactgc gtcagcagga gaagatcgaa cgctacgaag
    22621 cggatctcga tgagctgcag atccgtctgg aagagcaaaa cgaagtggtg gcagaagcca
    22681 tcgaacgcca ggaagagaac gaggctcgcg cggaagctgc cgaactggaa gtggacgagc
    22741 tgaaaagcca gcttgctaac taccagcagg cgctggacgt ccagcaaacg cgcgcgatcc
    22801 agtataacca ggcgattgct gcgcttaatc gcgccaaaga actgtgccat ctgccggact
    22861 taaccgccga cagcgccgcc gaatggctgg aaaccttcca ggcgaaagag ctggaagcga
    22921 ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc gcaaaccgcg cacagccagt
    22981 ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc gctggcgcgt aacgaggcgt
    23041 gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca gcgtcacctg gcagagcagg
    23101 ttcagccgtt gcggatgcgc ttaagcgaac tggaacagcg tctgcgcgaa cagcaagaag
    23161 ctgagcgtct gctggcagat ttctgcaaac gtcagggcaa gaattttgac attgacgagc
    23221 tggaagctct gcatcaggag ctggaagcgc gcattgcctc tctttccgat agcgtgtcta
    23281 acgcccgtga agagcgcatg gcactgcgcc aggaacagga acagcagcag tctcgcattc
    23341 agagtttgat gcagcgtgcg ccggtttggc tggcagcgca aaacagtctc aaccagttga
    23401 gcgaacagtg cggcgaagag tttacctcca gccaggacgt caccgaatat ctgcaacagt
    23461 tgctggagcg tgagcgagag gcgattgttg aacgcgatga agtgggcgcg cgcaaaaacg
    23521 ccgtcgatga agagatcgaa cgtttaagcc agcctggcgg ctctgaagat cagcgtctga
    23581 acgcgctggc ggagcgtttt ggtggtgtgc tgctgtcaga aatttatgac gacgttagcc
    23641 tggaagatgc gccgtacttc tcagcgctgt atggcccgtc acgccacgcc atcgtggtgc
    23701 cagatctgtc acagataact gaacacctgg aaggcttgac cgattgcccg gaagatctct
    23761 atctgatcga aggagatccg cagtcattcg atgacagcgt gttcagcgtt gatgagctgg
    23821 aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg ttattcacgt ttcccggaag
    23881 tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga aagcctccat gccgagcgtg
    23941 aagtgctttc cgaacgcttc gccacgctct cctttgatgt acagaaaact cagcgtctgc
    24001 atcaggcgtt cagccgcttt atcggcagtc atctggcggt tgcgtttgag tctgacccgg
    24061 aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact ggagcgggcg ttgagtaatc
    24121 atgaaaatga taaccagcag cagcgtattc agtttgagca ggcgaaagaa ggcgttacga
    24181 cgctgaaccg cattctgccg cgtctcaacc tgttggctga tgacagcctg gcggatcgcg
    24241 tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc cgcgcgtttt gttcagcagt
    24301 ttggcaatca actggcgaaa ctggaaccga tcgtttcggt attgcagagc gacccggaac
    24361 agttcgaaca gctaaaagaa gattacgcgt actctcagca gatgcagcgc gatgcccgtc
    24421 agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc gcactttagc tattctgact
    24481 cggcagaaat gctgagcggt aatagcgatc tcaacgaaaa gctgcgtgaa cgtctggaac
    24541 aggcggaagc ggagcgtact cgcgctcgtg aagcgttgcg tggtcacgca gcgcagttga
    24601 gtcagtacaa ccaggtgctg gcttcgctga aaagttctta cgacaccaaa aaagagctac
    24661 tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg tgctgatagc ggggcagaag
    24721 agcgggcgcg tattcgccgt gacgagctgc atgcgcaact gagcaataac cgttcacgcc
    24781 gcaatcaact ggaaaaagcg cttaccttct gcgaagcgga gatggacaac ctgacccgca
    24841 aactgcgcaa gctggagcgg gattactttg agatgcgcga gcaggtagtg accgccaaag
    24901 cgggctggtg tgcggtgatg cgcatggtga aagataacgg cgttgagcgc cgcttacacc
    24961 gtcgtgagct ggcttatctc tccgctgatg atttgcgctc tatgtcggat aaggcattgg
    25021 gtgcgctgcg tctggcggtg gcggataacg aacatctgcg cgacgtgctg cgcatgtcgg
    25081 aagatccgaa acgtccggag cgtaaaattc agttcttcgt ggcggtttat cagcatctgc
    25141 gtgaacgtat tcgtcaggat attattcgta ccgatgatcc ggtggaagct atcgaacaga
    25201 tggagatcga acttagccgt ctgaccgaag aattaacctc ccgtgaacag aaactggcga
    25261 tcagttcccg cagcgtggcg aacatcattc gcaaaaccat tcagcgcgag cagaaccgta
    25321 tccgtatgct caaccagggg ttgcagaacg tatcgtttgg tcaggtgaac agcgtgcgcc
    25381 tcaacgtgaa cgtgcgtgaa acgcacgcca tgctgctgga tgtgctctct gaacagcacg
    25441 agcagcatca ggatctgttt aacagcaacc gtctgacctt ctcggaagcg ttggcgaaat
    25501 tgtatcagcg tcttaatccg cagattgata tgggacagcg cacgccgcag accattggcg
    25561 aagaactgct ggattaccgc aactatctgg aaatggaagt tgaggttaac cgtggttccg
    25621 atggttggtt gcgcgcagag tctggtgcat tgtcgaccgg tgaggcgatt ggtaccggta
    25681 tgtcgattct ggtgatggtg gtacaaagct gggaagatga atctcgccgc ctgcgcggta
    25741 aagatatctc tccttgccgc ctgctgttcc tcgatgaagc agcgcgactg gatgctcgtt
    25801 ctatcgccac gctgtttgaa ttgtgtgagc gtttgcaaat gcagctcatc atcgcagcgc
    25861 cggaaaatat cagcccggag aaaggcacca cctataaact ggtgcgtaaa gtcttccaga
    25921 ataccgaaca cgttcacgtc gtcggcctgc gaggatttgc gccgcaactc cctgaaacgc
    25981 ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa ttaaggcggc agcaatgccg
    26041 ccttttcttt ttccgaaaac tccgtttccg cactaaaaaa gtggcacatt acggcgcgct
    26101 tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta tatactgaag
    26161 ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg cgttgtacag
    26221 gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg ggcaagggat
    26281 gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc tggccgtaac
    26341 attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg gcgacagccc
    26401 ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg caataatggc
    26461 ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa tcgaatctca
    26521 attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt atgccgcacg
    26581 cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc aacagctggc
    26641 agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt tactgaccga
    26701 tcctggtgtt aacgggatgg cacgcgacgt agtgctctct gatgcgatga tgggctatct
    26761 ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca gtaaacctta
    26821 tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc tggataaagg
    26881 tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg cggcgatgca
    26941 tgaatcgtta ctggccttac tcagtgacac caaaccgtgg ccccaactga ccggcaaagc
    27001 aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa tattgcaacg
    27061 cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca cgccaactga
    27121 cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc cgatggacaa
    27181 gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgt gccgcctacg ataatgaact
    27241 ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg gtgctattgg
    27301 cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg tgttggctct
    27361 caacatccag cgattgcgct tgctgccaac agagctttct accgggatca tggttaacat
    27421 tccggcctat tcgctggtct actataagaa cggcaatcag gtgctggatt cgcgagtcat
    27481 tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca acgtagtggt
    27541 aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc caaaagtgcg
    27601 caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgcggct ggaacagcag
    27661 agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga atttaccgtt
    27721 ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat tcaatatgcc
    27781 gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca agcgtgatac
    27841 acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg cgaatatgct
    27901 gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc agggtgatac
    27961 acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga cggcctttgt
    28021 tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc tgcctgcgcg
    28081 atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga agtagttcgg
    28141 ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag cccccgtcac
    28201 tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc tcttgtgcgc
    28261 cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg acaaattcga
    28321 cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg ccatcctgcc
    28381 gacccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac tcaataatct
    28441 tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata ttcaggaaga
    28501 attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa agtccatcga
    28561 cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc gcaaaccggt
    28621 gcaactcatt tccggttatc gttctattga taccaacaat gaactacgcg cccgcagccg
    28681 tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc atattgaagg
    28741 tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg gtgtaggata
    28801 ttatccacgt agtaactttg tgcatattga taccgggcca gcacggcact ggtagtaatt
    28861 gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc attctcccag
    28921 aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga tcctggcggc
    28981 gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat gcagatcctg
    29041 ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca acattacggc
    29101 gtgccggttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt gcctgcgcaa
    29161 agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg gctgaacgaa
    29221 ggcgatacca tcagcatagg gaatgtgact ttacaggtgt tacattgccc tgggcatacg
    29281 ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg cgatgttatt
    29341 ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca actgatttct
    29401 tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc gggtcacgga
    29461 ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga cgaaatgccc
    29521 gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc tggcaaagtg
    29581 cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa tcgtgcaaat
    29641 tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc aatgttgcgt
    29701 ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc ttacagcact
    29761 gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc cacatttacg
    29821 cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac ttgttctttt
    29881 gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa gtcgcggttt
    29941 gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat acgctggcgc
    30001 atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag gatggtggca
    30061 acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc cgctttcatt
    30121 tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca agcgccaaca
    30181 cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag ctctttatgc
    30241 atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc aaaaccctgg
    30301 taagcgaagt caaacagcgg taaccagcct ttctcaactg agagttgtgc cagtgtctgc
    30361 cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg gaacagcact
    30421 acgtcgccag cctgagcttc gttcaggctg ttaatcagtg catcgaagtc aagggtgtgg
    30481 ttttctgcat cataataagc gtattcacga acttccagac ctgcagagtt aaagacgctc
    30541 ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt attttttgcc
    30601 aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc cgtgcgagca
    30661 cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt gcagcgacca
    30721 aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc gagcagatac
    30781 tgttcagcct ttttcacgct ggtcaatacc ggggttttgc ccgtctcatc tttatagaca
    30841 ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc ggccaggccc
    30901 agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt ccattatggt
    30961 tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca actattactg
    31021 atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggcat aaaaaaaaca
    31081 ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga actggtaaac
    31141 gatacccaca gcaacggtgt cgtcagaacc tacgcccagt ttgttgtcag aatcgatctg
    31201 gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg ttgcgcccac
    31261 ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg ctttagattt
    31321 ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa ctaacagaac
    31381 gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga tcggcgtagc
    31441 gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt acttcagacc
    31501 agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt gcaggttggt
    31561 acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt agctgataga
    31621 accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt tacccaggta
    31681 ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac gataggtagc
    31741 aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac caccaaattc
    31801 tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac ggccgtaatc
    31861 gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt tgttaccagt
    31921 ttgagcgtca gcgccttcag agttgttacc ctggaagtta tattcccact gaccataacc
    31981 ggtcagatcg gaattgattt gagtttcccc tttaaaacca agacgggcat aggtcatgtc
    32041 gccattgcca ccgtaactgt tttcaccgtt acccttggaa aaataatgca gaccaacagc
    32101 tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag cgtttgcagt
    32161 acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca tcattattta
    32221 ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc tgtcaataaa
    32281 aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc atctttccat
    32341 tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag tttcaaaaag
    32401 aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc atatttccat
    32461 agaaacgtga tgatatttac agcaattttt atctattcat atgatttact tatatttaaa
    32521 ttaactaaac ggaaattttg tttctgatgg aaactttatc gaactggcac aaaatcctct
    32581 ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct aagaaaaaac
    32641 taatccgcaa taagaatgcg ggttattttt tgtgcgtaaa tgttgcaatg taatattccg
    32701 taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac aaacagataa
    32761 cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa gaaaatcaga
    32821 agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt tgcacgccag
    32881 ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga acagtaccgt
    32941 agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt tccagcatac
    33001 gctcgtccag aacgtccaga cgttcttcac gctgggagcc accaatgatc tcaccgatgc
    33061 ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg cgcatataga
    33121 acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag tgttcttccg
    33181 ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg ttttcaaact
    33241 tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc gcgaaatcgg
    33301 cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca gcgaagaatt
    33361 tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc atggcttcag
    33421 ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc atccagaatt
    33481 ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg aaggtataaa
    33541 ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg gtcaggaaag
    33601 actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta cgcggcaggt
    33661 tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta tcagatgcgg
    33721 taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga tgcagcgcct
    33781 gcgccagcgt atgacgaacg cgcgcgacgg caccaatcag gtttgtgcgc ggacgcaggt
    33841 gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg taagtgtctg
    33901 gatcttcaac ccaaccagca acttcaacct tgctggcctg aatttcaaat tgttgcccct
    33961 ggcccggcga cgccacgact ttacccgtca caatgaccga gcagccggtg gtcagacgca
    34021 ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca ggatcaaagc
    34081 aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg cgggtacgta
    34141 cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt acgtcggcta
    34201 caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa cacttgtcca
    34261 cccgtaaatg gggggatacc tatgttacct ggcatctgca atcagacaag cagaattcgc
    34321 aaatgcagcg aaagatttcg gaattaaagg taaaaaaagg gagccgatta gctccctgat
    34381 gatattaact ggctttttta atatgcggaa ggtcgaacgc tttgcgcagc gcccgaacaa
    34441 acgctttatc gtggcagata gttttgccag ggctgtcaga aagtttcgcc accggtttac
    34501 cgttacactc aaccaactta atcacaatat tcaggggttt tacctggggg atatcgcagg
    34561 tcaggcgagt cccaatacca aaacttaatt gcacgcggga agagaagtgg cggtatagct
    34621 caaccgcttt gcgtaaatcc agattgtcag agaaaaccag agttttactc tgtggatcaa
    34681 ttcccagctt ttcataatgt gcaatggctt tttcacccca ttcaaccggg tcgccagagt
    34741 catgacgcag gccctgatag cgactggcga actcgacacc gaaatcacgc aggaaagcat
    34801 ccatagtgat gcagtcggtt aatgcaatgc caagttggtc gggatattct tccagccagg
    34861 cagcaagtgc agctcgctgg ctgttggcta gatccgggct gatttgctga tgtgcctgga
    34921 accattcgtg tgcctgtgtt cccatcggcg tgagggaaag ccgacgcgcc agatcgtagt
    34981 tgctggtgcc cacgaaccag gattcctgtt gcagacgctt aacgatggtt tcttgtactt
    35041 cgcgagaaaa acggcgacgg gtgccaaaat ccataagatg gaagcgcgac atatcaaggc
    35101 cggcggttaa cgccgagaag tcgactaatt tgctttccag cgtgtcgagg gcttgcgcaa
    35161 cgtcggcctg cggtgagcga tagcgatgta ccatttcact gataaccgcc agcaaaggaa
    35221 cttcccagag gatgacttca cgccacgggc cgcttaaacg aatatccagc ttgccattat
    35281 cgttggatac ggtgacttgt tccgggttaa agcggaactc gcgtaaccag ttaagatagt
    35341 cggccttaaa gaaaggcagg gcagagagcc actgatattc atcatcctgc aggcgcaggt
    35401 gctgcatcgc ctgaatctgt tcacgaatag catcggcata aatacccagc agatcgtcac
    35461 ctcggcaacg aaactccgcc gcgacatgca catcgtaata gtgatgaaac acggcttgct
    35521 gcatatgcaa cttataagca tctgtatcca gcaacgagtg cagaacagga gaagcgaatt
    35581 gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt tagtacaata aacatcttca
    35641 ggaactgctg gagtatacct tgtttcgcaa tttattgaac cccgatcaca ccatatgcca
    35701 cctttctggt cgatggcatt acgcggtgta tgttataaaa atgtagcaat aaaggcgttt
    35761 gtacctgaaa agatgaagat tctgcatagc gcgatttacg caacaggaat agactgaaca
    35821 ccagactcta taaaagatgc taaaggttat ttatgactca acagccacaa gccaaatacc
    35881 gtcacgatta tcgtgcgccg gattaccaga ttactgatat tgacttgacc tttgacctcg
    35941 acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt ccgtcatggt gcatcagatg
    36001 ctccgcttcg tctcaacggc gaagacctca aactggtttc tgttcatatt aatgatgagc
    36061 cgtggaccgc ctggaaagaa gaagagggcg cactggtcat cagtaatttg ccggagcgtt
    36121 ttacgcttaa gatcattaat gaaataagcc cggcggcgaa tacggcgctg gaagggcttt
    36181 atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc catattacgt
    36241 attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt gccgataaaa
    36301 ccaaatatcc cttcctgctt tccaacggta accgcgttgc gcaaggtgaa ctggaaaacg
    36361 gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg tttgcgctgg
    36421 tggcaggcga ctttgatgta ctgcgcgaca cctttaccac gcgttctggt cgcgaagtag
    36481 cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg atgacctcgc
    36541 tgaaaaactc catgaaatgg gatgaagaac gctttggcct ggagtatgac ctcgacatct
    36601 atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag ggtctgaata
    36661 tctttaactc caaatatgtg ttggcccgca ccgacaccgc caccgacaaa gattatctcg
    36721 atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac cgagtgacct
    36781 gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc gatcaggaat
    36841 tcagctctga ccttggttcc cgcgcggtaa accgtatcaa taatgtgcgc accatgcgcg
    36901 gattgcagtt tgcggaagac gccagcccga tggcgcaccc gatccgcccg gatatggtca
    36961 ttgagatgaa caacttctac accctgaccg tttacgagaa gggcgcggaa gtgattcgca
    37021 tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc tatttcgagc
    37081 gccatgatgg cagcgcggca acctgcgacg actttgtgca ggcgatggaa gatgcgtcga
    37141 atgtcgatct ctcccatttc cgccgttggt acagccagtc cggcacgccg attgtgaccg
    37201 tcaaagacga ctacaatccg gaaaccgagc agtacaccct gaccatcagc cagcgcacgc
    37261 cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccatttgcc atcgaattgt
    37321 atgacaacga aggcaaagtg atcccgttgc agaaaggcgg tcatccggtg aattccgtgc
    37381 tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc cagccggtgc
    37441 ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg agcgatcagc
    37501 aactgacctt cctgatgcgt catgcgcgta atgatttctc ccgttgggat gcggcgcaaa
    37561 gtttgctggc aacctacatc aagctgaacg tcgcccgtca tcagcaaggg cagccgctgt
    37621 ctctgccggt acatgtggct gacgctttcc gcgcggtgct gctcgatgag aagattgatc
    37681 cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct gaactgttcg
    37741 atatcatcga cccgattgct attgccgaag tacgcgaagc actcactcgt actctggcga
    37801 ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc gagtaccgtg
    37861 ttgagcatga agatattgca aaacgcactc tgcgtaatgc ctgcctgcgc ttccttgctt
    37921 ttggtgaaac gcatctggct gacgtgctgg tgagcaagca ataccatgaa gcaaacaata
    37981 tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc acagctgcct tgccgtgacg
    38041 cgctgatgca ggagtacgac gataagtggc atcaggacgg tctggtgatg gataaatggt
    38101 ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgt ggtctgttgc
    38161 agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt ggtgcgtttg
    38221 cgggcagcaa cccggcagcg ttccatgccg aagatggcag cggttaccag ttcctggtgg
    38281 aaatgcttac cgacctcaac agccgtaacc cgcaggtagc ttcacgtctg attgaaccgc
    38341 tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg gcgctggaac
    38401 agttgaaagg gctggaaaat ctctctggcg atctgtacga gaagatcacc aaagcactgg
    38461 cttgatactt ttgagccaaa atgcctgatg cgacgcttga cgcgtcttat caggcctaca
    38521 actccgttga aattgctggt tttgtaggct ggataaggcg ttcacgccgc atccggcagc
    38581 cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc aggcctacta gtccgttgaa
    38641 aatgctggtt ttgtgcgcca catccggcat attaaccatg acgccgaatc agttgttcag
    38701 aatgcccacg ccgcattacc cgttgcaata cttccgcttc cagctccgct aatcgcaccg
    38761 accctaatcg acgcggacgg gggatatcca ctgtcaaatc cagaccaatt tttccctctt
    38821 caattaacaa cacccggtca gccattgcta ccgcttcgct cacatcatgc gtcaccaaca
    38881 gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa atcctgcatc tcgagtcgcg
    38941 ttaaggcgtc cagcgccccg agcggttcat caagcaacaa taaacccggt cgatgaatca
    39001 acgctcttgc cagcgccaca cgctgtttct gcccgccaga aagtgcggca ggccattccc
    39061 cggcgcgatt ctccagtcct actgcagcca acgcccgacg cgcggcatcg cgccactggc
    39121 ctttaaggcc taacccaacg ttatcaatca ccgatttcca cggcagcaga cgcgcatcct
    39181 gaaacatcat tcgcgtgtct tcctgaatct cagccagcgg tgtggtgccc gctaacacat
    39241 cgcctgcggt tggcgtttcc agacctgcca gcaggcgcag cagggtactt ttgccaccac
    39301 cgctgcggcc caccaccgcc acaaactgac ctgccggaat atgtaaatcc agttggttca
    39361 ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt gtgccctggt
    39421 tcagacgagc agtattcata ccgtggcctc cttcaaatga taagccgggt tccagcgcaa
    39481 ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca gggcgtaaag
    39541 aataatagcg actaccacca cgtccgtttg caaaaactcc cgcgcattca tcgccagata
    39601 accaatgcct gaattggcag aaatggtttc ggcaacaatc agcgtcagcc acatcaggcc
    39661 caacgcaaaa cgcacgccga ccataattga gggcagggca ccaggcagga tcacatggat
    39721 aaacagcggt atgccggata atccatagct acgcgccatt tccaccagac cgcgatcgat
    39781 attacggatg ccatgccagg tgttgatata aatggggaat agcgtgccga gcgccaccag
    39841 aaagattttt gcggattcat caatgccaaa ccacaaaatc accagcggga tcagcgccag
    39901 atgcggtacg ttgcgcaaca tctgaattga ggtatccagc agccgttctc cccagcgaga
    39961 tagcccgcta atcagtccca aaatcaaccc cagcgatccg ccaattgaaa agccaatcag
    40021 cgcccgccag gaactgatcg ccagatgctg ccacagttcg ccgctggcgg agagcgtcca
    40081 gaacgccgtc accacccctt ccggtgaggg caaaatacgc gtcgacagcc agccaactga
    40141 ggaggccagt tgccacaccg ccacgatgcc caccggtaaa aaccaggggg caacgcgcaa
    40201 taaccacttc ttcactggcg ttgccattat ttcttcctta gctttgcgcg actttacggg
    40261 ggataaaatc attcgccacc gcttcgcctt gcggattcag cggctgcggc tggggaattt
    40321 ccggaatggc gacatccaga tgtgggaaca gcaactcgcc gacccgatac gcttcttcca
    40381 gatgcggata gcccgaaagc acaaagctgt cgattccaag cgcggcgtat tcgttgattc
    40441 gcgcagcgac cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg cgcactaagc
    40501 caacgcccgc ccataaattg gggctgatct ccagattgtc gcgcttgccg ttatgtaacg
    40561 ccgccattcg ctgttgtcct acggaatccg tccgggcgaa tgcggcctgt gctttggcga
    40621 tagtttcatc atcaagatgc gagattaacc gctcggcggc ctgccacgct tcgtcgttag
    40681 tttcacgaac aatcacatgc agacgaatac cgaaacgaat tttgcgtcca tgcgcggcag
    40741 ctttcgcccg cacttgttcg atcttctctt ttactagttc tggcggttcg ccccaggtga
    40801 ggtagagatc aacttgttct gccgccagct cctgggcaac gtctgacgat ccgccaaagt
    40861 aaagcggagg ataaggctgt tgaatcggcg ggaagagcag ttttgctccg cgcacatgaa
    40921 tatgtttacc gttgaaattg acggtttccc ccagcaataa gcgccgccag acctgggtaa
    40981 attccgccga ggcttcgtag cgctcgctat gatcaaggaa cacgccgtcg cctgccagct
    41041 cttgtggatc gctgcctgtg accagattaa acaacgcacg accatttgag agacggtcaa
    41101 gcgtggcggc ctggcgagcg gcaacggtag gtgaggttac gctggggcgc agggcgacaa
    41161 gaaacttcag ccgctgcgtc accgggatca tcgacgccgc caccagccac gcatcttcac
    41221 acgatcgccc cgttgggatc agcacaccgg tatagcccag acgatccgcc gcttgcgcaa
    41281 tttgttgcag atagccgtga tcaaccgggc gtgaaccttc ttccgttcca agataatgcc
    41341 cgtcaccatg ggtcggtaaa aaccagaaca tattcagact cataattgtt ttccttccag
    41401 ttgagtgggc tgccagatgc gctggcgaat atcgactttt ttcggcacca gacgattttc
    41461 ataaaacaga tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa ccggtttgat
    41521 ggtggtagga gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta agcccatcgt
    41581 ttttgccagt aaagcgatgc tttgctcgcg ctggctgcgg gttaacgcat cggcctcact
    41641 aaaggttgcg agtacgccct gaataaaagc gccgtttttt tctgcatagg gacgagctgc
    41701 cagataaaac gatccagttt gattgagatc ggtgccgtct ttcagcaccc gcacggcgcc
    41761 ctgtaataat gcagcagagt agtagggatc ccagatagcc caggcgtcaa cgttaccttg
    41821 ctggaacgcg gcgcgggcat cagcgggcgt caggtaagtg ggttggatat cggtaaactt
    41881 aagtccggcc tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac ctttctgaaa
    41941 ggcaactttg tgacctttaa gatcggctac ggttttgatc gggctgtttt ctgccaccag
    42001 aatcacttcg gctttgggct tcggtggctc gacgcccaca tataccaaat cagccccggc
    42061 agcctgggca aagattggcg gaatatcccc ggtactgccg agatcaatac tgccaacgtt
    42121 taacgcttcc aacatttgcg gacccgcggg gaactccacc caggagattt ttgattgcgg
    42181 atagcgtttt tccagtaact ggtggctttt tgccaatacc ataccaatac tgcctttctg
    42241 atagcctata cgtaacgctt caggcgagga ttctgcagca accgcaaaag tagagacgct
    42301 aagcaatccc gccagcgcca gtttaatgat gttacgcatg gacattacct cgcagagaca
    42361 gaaggtcagg aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt tctagcgcgg
    42421 tatcaagacg ggtttgcaga tttggcgtga actggggttt gtgatggtaa tcaataactt
    42481 gtgagtcatc ggcaaacacg ccgtgcagga tctcctgggc tttcatcgca cttaaaactg
    42541 gcttaagggc ataatcgacc gccagcagat gagccaccgt accgcccgtc gccagcggta
    42601 gcaccacttt gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt ttcagcgcgc
    42661 cggaataggc ggctttatac acaggtgtag cgactatcag tccatcagcc tgttgcagct
    42721 gttcggtgaa ggtcttgagt gccggactat cgaaacgagc gtaaagcaga tcttccgggg
    42781 cgaagttttg cagattccag tgataaacct ctacatccag gcaatttagt ttttcccgcg
    42841 catattccag caaggagctg gagcgagaag gaaagcgagg actacccgcc agggtgatga
    42901 cacgcatact ctctccttat aaccaattgt tctttttttg ttaacattga taacaattcg
    42961 gtcagtctgt cggagagaca agaaaatgcc aaatataaat tttgtgtatc tttttctgaa
    43021 aataaacta
//
LOCUS       DAZUFB010000032        41467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZUFB010000032 DAZUFB010000000
VERSION     DAZUFB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.032"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1395..1943)
                     /locus_tag="ACHY3M_001956"
     CDS             complement(1395..1943)
                     /locus_tag="ACHY3M_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4316882.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRDMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVESLQTKGVRVVSLREGFDLS
                     TPIGKAILTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISKATYYRLAK"
     gene            2739..3035
                     /gene="yciI"
                     /locus_tag="ACHY3M_001957"
     CDS             2739..3035
                     /gene="yciI"
                     /locus_tag="ACHY3M_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFS4316883.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQSWADADPYVAAGVYEHVSVKPFKKVF"
     gene            3334..4587
                     /gene="kch"
                     /locus_tag="ACHY3M_001958"
     CDS             3334..4587
                     /gene="kch"
                     /locus_tag="ACHY3M_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFS4316884.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(4642..4815)
                     /gene="yciY"
                     /locus_tag="ACHY3M_001959"
     CDS             complement(4642..4815)
                     /gene="yciY"
                     /locus_tag="ACHY3M_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFS4316885.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            4958..6418
                     /gene="cls"
                     /locus_tag="ACHY3M_001960"
     CDS             4958..6418
                     /gene="cls"
                     /locus_tag="ACHY3M_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4316886.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            6453..6782
                     /gene="yciU"
                     /locus_tag="ACHY3M_001961"
     CDS             6453..6782
                     /gene="yciU"
                     /locus_tag="ACHY3M_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFS4316887.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6835..7839)
                     /gene="oppF"
                     /locus_tag="ACHY3M_001962"
     CDS             complement(6835..7839)
                     /gene="oppF"
                     /locus_tag="ACHY3M_001962"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFS4316888.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7836..8849)
                     /gene="oppD"
                     /locus_tag="ACHY3M_001963"
     CDS             complement(7836..8849)
                     /gene="oppD"
                     /locus_tag="ACHY3M_001963"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFS4316889.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(8861..9769)
                     /gene="oppC"
                     /locus_tag="ACHY3M_001964"
     CDS             complement(8861..9769)
                     /gene="oppC"
                     /locus_tag="ACHY3M_001964"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFS4316890.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(9784..10704)
                     /gene="oppB"
                     /locus_tag="ACHY3M_001965"
     CDS             complement(9784..10704)
                     /gene="oppB"
                     /locus_tag="ACHY3M_001965"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFS4316891.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(10790..12421)
                     /gene="oppA"
                     /locus_tag="ACHY3M_001966"
     CDS             complement(10790..12421)
                     /gene="oppA"
                     /locus_tag="ACHY3M_001966"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFS4316892.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLNGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            12705..13001
                     /locus_tag="ACHY3M_001967"
     CDS             12705..13001
                     /locus_tag="ACHY3M_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316893.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(13159..13806)
                     /gene="ychE"
                     /locus_tag="ACHY3M_001968"
     CDS             complement(13159..13806)
                     /gene="ychE"
                     /locus_tag="ACHY3M_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFS4316894.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(13837..13890)
                     /gene="ychE"
                     /locus_tag="ACHY3M_001969"
     CDS             complement(13837..13890)
                     /gene="ychE"
                     /locus_tag="ACHY3M_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFS4316895.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            14283..16958
                     /gene="adhE"
                     /locus_tag="ACHY3M_001970"
                     /gene_synonym="adhC"
     CDS             14283..16958
                     /gene="adhE"
                     /locus_tag="ACHY3M_001970"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4316896.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            17082..17978
                     /locus_tag="ACHY3M_001971"
                     /pseudo
     CDS             17082..17978
                     /locus_tag="ACHY3M_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(18260..18877)
                     /gene="tdk"
                     /locus_tag="ACHY3M_001972"
     CDS             complement(18260..18877)
                     /gene="tdk"
                     /locus_tag="ACHY3M_001972"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4316897.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            19482..19895
                     /gene="hns"
                     /locus_tag="ACHY3M_001973"
     CDS             19482..19895
                     /gene="hns"
                     /locus_tag="ACHY3M_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFS4316898.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(20039..20947)
                     /gene="galU"
                     /locus_tag="ACHY3M_001974"
     CDS             complement(20039..20947)
                     /gene="galU"
                     /locus_tag="ACHY3M_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4316899.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21149..22162)
                     /gene="rssB"
                     /locus_tag="ACHY3M_001975"
     CDS             complement(21149..22162)
                     /gene="rssB"
                     /locus_tag="ACHY3M_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFS4316900.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(22254..23159)
                     /gene="rssA"
                     /locus_tag="ACHY3M_001976"
     CDS             complement(22254..23159)
                     /gene="rssA"
                     /locus_tag="ACHY3M_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFS4316901.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            23272..23730
                     /gene="ychJ"
                     /locus_tag="ACHY3M_001977"
     CDS             23272..23730
                     /gene="ychJ"
                     /locus_tag="ACHY3M_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFS4316902.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            23780..24622
                     /gene="purU"
                     /locus_tag="ACHY3M_001978"
     CDS             23780..24622
                     /gene="purU"
                     /locus_tag="ACHY3M_001978"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFS4316903.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            24782..24866
                     /locus_tag="ACHY3M_001979"
     tRNA            24782..24866
                     /locus_tag="ACHY3M_001979"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24816..24818,aa:Tyr,seq:gta)
     gene            24914..25045
                     /locus_tag="ACHY3M_001980"
     ncRNA           24914..25045
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_001980"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            25092..25223
                     /locus_tag="ACHY3M_001981"
     ncRNA           25092..25223
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_001981"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(25407..26084)
                     /gene="narI"
                     /locus_tag="ACHY3M_001982"
     CDS             complement(25407..26084)
                     /gene="narI"
                     /locus_tag="ACHY3M_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS4316904.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(26084..26794)
                     /gene="narJ"
                     /locus_tag="ACHY3M_001983"
     CDS             complement(26084..26794)
                     /gene="narJ"
                     /locus_tag="ACHY3M_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS4316905.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYGVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(26791..28329)
                     /gene="narH"
                     /locus_tag="ACHY3M_001984"
     CDS             complement(26791..28329)
                     /gene="narH"
                     /locus_tag="ACHY3M_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS4316906.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(28326..32069)
                     /gene="narG"
                     /locus_tag="ACHY3M_001985"
     CDS             complement(28326..32069)
                     /gene="narG"
                     /locus_tag="ACHY3M_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFS4316907.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(32584..33975)
                     /gene="narK"
                     /locus_tag="ACHY3M_001986"
     CDS             complement(32584..33975)
                     /gene="narK"
                     /locus_tag="ACHY3M_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFS4316908.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            34314..36109
                     /gene="narX"
                     /locus_tag="ACHY3M_001987"
                     /pseudo
     CDS             34314..36109
                     /gene="narX"
                     /locus_tag="ACHY3M_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
     gene            36102..36752
                     /gene="narL"
                     /locus_tag="ACHY3M_001988"
     CDS             36102..36752
                     /gene="narL"
                     /locus_tag="ACHY3M_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFS4316909.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(36753..38147)
                     /gene="ychO"
                     /locus_tag="ACHY3M_001989"
                     /gene_synonym="iatA"
     CDS             complement(36753..38147)
                     /gene="ychO"
                     /locus_tag="ACHY3M_001989"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFS4316910.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            38332..38685
                     /gene="ychN"
                     /locus_tag="ACHY3M_001990"
     CDS             38332..38685
                     /gene="ychN"
                     /locus_tag="ACHY3M_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFS4316911.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(38729..39424)
                     /gene="chaC"
                     /locus_tag="ACHY3M_001991"
     CDS             complement(38729..39424)
                     /gene="chaC"
                     /locus_tag="ACHY3M_001991"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFS4316912.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(39582..39812)
                     /gene="chaB"
                     /locus_tag="ACHY3M_001992"
     CDS             complement(39582..39812)
                     /gene="chaB"
                     /locus_tag="ACHY3M_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFS4316913.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            40082..41182
                     /gene="chaA"
                     /locus_tag="ACHY3M_001993"
     CDS             40082..41182
                     /gene="chaA"
                     /locus_tag="ACHY3M_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFS4316914.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT        10676 a        10527 c         9720 g        10544 t
ORIGIN      
        1 ctctgacagt aatttctaaa agttcttatt tttcaataag ttgtaattat ccatatatag
       61 agaactgaca agtctcttca cagttaactc tgtaacctta acagcaacct ctaaaaacac
      121 ttatttttca atgagttgta atcacccaat atataaggct gtccagcacc ttcacagaga
      181 aacttcagag gtcttcttag ttgggcttgt ataacacaac ccctaaccgt ccactaagcg
      241 ggagaagagc gacacccaag aaagggaagc tcccttctaa ttggtattgt taccaatccc
      301 taaaccgacc ttcttaacca gccctttacg ttattcgttc acagtgttga taagtggttt
      361 tgccctctgt ttctgttatc atcgggctta ccttatcagg gctttgaaat gccctgtaac
      421 tcccctctta acgggagaga aagaacatag tgaccccaag cgaagcgccc ctctaaaaag
      481 attttttagc tattttgctt aaaaacacat caaacgatac attttattga tttaggtcaa
      541 agacaaaagc attgacaaaa tggtaagatt gttcacatga ccttacttaa cagattctta
      601 agagctttga aacgaactgt ttaacaggtc actacctctg gtcttatcgt tccctctctt
      661 cttggtagtt ctgttaagag gaacttttca ggtatcatct tacagtcctg ttaagataga
      721 acctaccttc ggtttcatgg ttctcttcct cccgttaaac aattcttcct aacaacttca
      781 tagttatctg tcaagtacct ttgaagattc tatcaaagcc ctctaaatct gacctgatat
      841 agatgctgtg aaggcatgtt ctctaggtct gtgtagctta cttagtggga aaagaggata
      901 tggtgaccat aggggaaccc ctcctgttaa aagtttcttg atgttatcat cacaatatgt
      961 ataatcacca actgtacaga caccttaacg gtttctctac acatccctat ggctaggtag
     1021 ggtgtggttt agcttggtac ggctaggtta ggtctggttt ggttaggtat gggtggattg
     1081 tcgtagccac gttaaaaatc gactatgagg tttatttact aaaccgaatc aactatgagg
     1141 gactcttcat gaagtaattc attactgaat tacctacggg agtccttttt agtttgtata
     1201 aaatctgtac aggagttatc aggcttctaa aatttttata aaaaataatc agacctcttc
     1261 taagtctctt caaccaaatc tttcaaaaat atctgacaga tgtctgtagc tgctaagcac
     1321 ctgttacacc agctatgtga ccccctttgc aaaaaagcca aatatcagtc acatccatgt
     1381 aactgttcag taacttattt agcaagtctg taataagttg ctttagagat tcctagctct
     1441 tcctgaatct gcaaccgtgt cttaccttgt gaaataagcg tctgtacagc ctctgaagaa
     1501 gcttttacag gcctccccat atgtacccct tcagctttag cacgttctat tccagccttt
     1561 ctacgttcac tgataaggtc tttttcaagg tcagctaaac cagccatcat agtaagtatt
     1621 gctttaccga ttggtgtaga caggtcaaaa ccttcacgta gagaaacaac cctcacacct
     1681 ttagtctgta gtgactccac gttagagagc acatcaacgg tattacgccc taaacggtca
     1741 atagctacca caactaacgt atcgtcacac cttacatagt tcatcatgtc tctaaaacca
     1801 tctctgctgg tggcctttgt agctcctgag acagcctcat caataaacca tttatcgacc
     1861 ttgtgaacct tctctatctg tagtttctgg ttgtctacag tctggtcttg tgttgatacc
     1921 ctgcaataac caatcaccgc catacatccc ccttagatgt ctcaaaagtt agtctcataa
     1981 gtttagctgt ctcataatcc aaaaacaacc ttttgagact acttgagggt gcattctacc
     2041 gttacgttga aagtgtctca aaaggaagtc cttttaagac tggttatgaa ggagctatga
     2101 ggttgatacc gaatcaacta tgaagatttt aggagggtaa ctataaccat cactttctaa
     2161 ctttcacgca ggaaactgtt aagtttcttg atagttatac catgtgacca actgcataga
     2221 tagcgttata tatccctgta tatagcactt ttctaggagt cgagagagga acgactaaca
     2281 cgatacagtt tatcctgaaa agggacttca cagattctct atatatttca ataagttagg
     2341 ataaaaagag actataccga tactgcatag aatctgtgta aaactgctta gatacttgga
     2401 aatctattat tagattgcat agtcataggg gttggaacaa gcccaactac acctgatatt
     2461 gtcctgtttt taacaggtct cttagaaaac cgaatagact tcattcaagg gtcattgaag
     2521 tcataatcat ttcagtagaa aaaccaggtc tcgattttaa atgcaaatag caatcatatt
     2581 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttaat
     2641 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
     2701 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
     2761 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
     2821 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
     2881 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
     2941 tggaagcagc tcagtcctgg gccgatgctg acccgtacgt agcggcagga gtctatgaac
     3001 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     3061 ggtttttgca accgtgttct tgtttggcct ttccttatag ccggtcagtg cattcaccac
     3121 cacaagcaga taataacttt atctgtattt ctttatatga tgtgaacata ttcaggaaaa
     3181 tgccctttcc tgacgcagag ttgctcgctc aaaacctttg attactgccg ataaagtcat
     3241 ctactattgt ttaacacatt cacgcgctgc atgtatgttc agaaaatgtg cacgctaaga
     3301 taattaaaca aaattgtggt tacagaggta tcagtgagtc actgggctac attcaaacaa
     3361 acagcaacaa atttatgggt gacccttcgg cacgacattc tcgcgctggc cgtcttttta
     3421 aatggattgc ttatttttaa aacaatctat ggtatgtcgg tcaatttgct tgatattttc
     3481 catatcaaag cattttcaga gttggatctc tccttgctgg caaacgcccc actatttatg
     3541 ctcggcgtct ttcttgtcct gaactccatt ggcttactgt tccgggcaaa gctcgcatgg
     3601 gcaatcagta tcattttgtt gttgatagcg ctaatttaca ccctgcattt ttatccctgg
     3661 ctgaaattta gtattggatt ttgcattttt acgctggtgt ttttgctgat actgcgcaaa
     3721 gacttctccc acagtagcgc cgcagccggg acaatttttg catttattag tttcacgaca
     3781 ttactgtttt actcaaccta cggtgcgctt tatttaagcg aaggttttaa tccgcgaata
     3841 gaaagtttga tgaccgcgtt ctatttttcg atagaaacca tgtcaaccgt cggctatggc
     3901 gatattgtcc ctgtttctga atcagcacga ttgttcacta tttcggtcat tatttccggc
     3961 attaccgttt ttgccacatc tatgacctca atttttggtc cgcttatccg cgggggattc
     4021 aacaaacttg taaaaggaaa caatcataca atgcatcgta aagatcattt tattgtttgc
     4081 ggacattcga ttctcgccat caatacgatc ctgcaactga atcaacgcgg acaaaacgta
     4141 acggttatca gcaacttgcc tgaagatgat atcaagcaac ttgagcaacg cttaggcgat
     4201 aacgctgatg ttatccccgg tgacagtaat gacagttcag tattaaagaa agcgggaatc
     4261 gatcgttgcc gggccattct ggcgctgagt gataacgatg cagataacgc gtttgttgta
     4321 ctctcggcaa aagatatgag cagtgatgtc aaaacagttc tcgccgtcag tgatagcaaa
     4381 aacctgaata agattaagat ggtacatccg gatatcattc tctcaccgca actgtttggc
     4441 agcgaaattc tggcgcgagt tttaaatggt gaagagatta ataatgatat gctcgtttca
     4501 atgttgttga actccggtca tggcattttc agcgataacg atgaacaaga aacgaaagct
     4561 gacagtaaag aatcagtgca aaaatagagt ttaaagacga aagagtaaac ttattgggca
     4621 ccgcaatggt gcccagtata tttagatatt gtagatcgca aacaataacg agttacgctg
     4681 tttgtttaga atgcacttcc tgatgctgtg gatacgcata tttcggcgcg attgcccctc
     4741 aagccaacgc gatttacgtc ggctagcctg acgctgcatg cgccagcgcc ccacttccgt
     4801 tctactccgc ttcatgttaa ctactctatg caataacaga atggtcatta tacccccacc
     4861 cccgacgctg accagtgctt ttcccgcgtt tttatttgcc agatgaatcc atatgcgtaa
     4921 actcataaca atgcgctttc aaaaggattt ctaatctatg acaaccgttt atacgttggt
     4981 gagttggttg gccattctgg gatactggtt gctcattgca ggcgtaactt tacgcattct
     5041 aatgaaacga cgcgcagttc cctccgcgat ggcctggctg ttgattattt acattctgcc
     5101 gttagtcgga attattgcct atcttgccgt tggcgagctc catttaggca aacgccgcgc
     5161 tgagcgcgcc agagcgatgt ggccttccac cgcaaaatgg cttaacgacc ttaaagcctg
     5221 taagcatatc ttcgccgaag aaaatagcag tgtcgctgcg ccattattca agctttgcga
     5281 gcgtcgtcag gggatcgctg gggtcaaagg gaatcagcta caactgatga ccgagtcaga
     5341 tgatgtgatg caggcgttaa tccgcgacat ccagctcgcg cgccataata ttgagatggt
     5401 gttttatatc tggcagcccg gcggcatggc ggaccaggtg gctgaatcat taatggcggc
     5461 tgcacggcgt ggtattcatt gccgattgat gctcgactcc gccgggagtg tggctttttt
     5521 ccgcagcccg tggcccgagc taatgcgtaa tgccggtatt gaagtggtcg aagccttaaa
     5581 ggtcaatctg atgcgtgtgt ttttacgccg tatggacctg cgccaacatc gcaagatgat
     5641 catgatcgat aattacatcg cgtacaccgg cagcatgaat atggtcgatc ctcgctactt
     5701 caaacaagat gcgggcgtag ggcaatggat tgatctgatg gcgcgtatgg aaggccccat
     5761 cgccaccgcg atggggatta tttattcctg cgactgggag attgaaaccg gaaaacgtat
     5821 tctgccgcca ccaccagatg tcaatattat gccgtttgaa caggccagcg gtcacaccat
     5881 tcacacaatt gcttctggcc ccggctttcc ggaagatctc attcaccagg cattattgac
     5941 tgcggcttat tcggcgcgtg aatatttgat catgaccacg ccctactttg tgccaagcga
     6001 tgatttactt catgcgattt gcacggcggc gcagcgcggg gtggatgtca gtattatcct
     6061 tccgcgaaaa aatgactcga tgctggtcgg ctgggccagt cgcgcattct ttacggaact
     6121 gcttgctgct ggggttaaaa tttatcagtt tgaaggcggg ttactgcata ccaagagcgt
     6181 gctggtcgat ggcgaactaa gtctggttgg caccgttaac cttgatatgc ggagtctgtg
     6241 gctgaatttc gagattaccc tggcaatcga cgataaaggt tttggtgcag accttgccgc
     6301 cgttcaggac gattatattt cgcgttcacg tctgctcgat gcccgtttat ggctaaaacg
     6361 tccattatgg caacgtgtcg ccgagcgact gttttacttc ttcagtccgt tgctgtaaaa
     6421 cgtcgccaac agaggttaaa caggtagtga ctatggatat ggatctaaac aatcgcctga
     6481 ctgaagatga aacgcttgag caggcctacg atatttttct cgaactggct gccgacaatc
     6541 tcgatccagc tgatgttctg ctgttcaatc ttcagtttga agagcgcggc ggcgcggagt
     6601 tattcgatcc ggcggaagac tggcaggaac atgttgattt cgatttgaac ccagactttt
     6661 ttgccgaagt ggtgattggt ctggcggaca gtgaagatgg tgaaatcaac gatgttttcg
     6721 cccgcattct gttatgccgc gaaaaagatc acaaactttg ccatatcatc tggcgggaat
     6781 aaataatcgc ttttagcgtg aaaaagggct gacaactgtc agcccttatt gtgtttaaag
     6841 cggatcgact ttcaggcaag aaacggcgtg tctgaaactc ccctccagaa caggacgtgt
     6901 tttggcgcac tccggaccgg caatcgggca acgggtacgg aaaacacaac cggaaggcgg
     6961 gttgatcggc gacggtaatt ccccttccag taactggatg gttttgttct tctccagatc
     7021 cggatcaggt atggggactg ccgacatcaa tgccctggtg taaggatgta gtggattgtg
     7081 gtagacctca tcataggtcc ccagttctac cgcatggccg agatacatca ccaacacacg
     7141 atcggaaatg tgttttacca cggccaggtc atgagcgata aaaattaatg acaatcccat
     7201 ctcacgttgc agctgctgga gcaggttgac cacctgcgcc tgaattgaca cgtccagcgc
     7261 cgaaaccggc tcatcgcaga taatcagctt cggttcaaga ataagagcac gagcaatccc
     7321 gatacgctgg cactgcccac cggagaactc atgcggatag cggttaatca ggttaggcaa
     7381 taacccgact ttcagcatca tcgccttcac gcgctcgcga acttcctggc gtgacatttt
     7441 cggatgataa gtacgcagtg gttcagcgat gatctcgccg atggtcatac gcgggtttag
     7501 cgatgccaac ggatcctgga aaatcatctg aatatcactg cgaacggcac gccattcatc
     7561 gggcttcatg cccagcaact ctttacctaa ccaggcaaca tgaccgtcgg tcgccttgac
     7621 caaaccgatg atggcgcgag caaaggtgga cttaccgcat cccgattccc ctaccacacc
     7681 taatgtttcc ccttcataca ggcgaagagt gacaccatcg acggctttga gcgttttcgg
     7741 cggttgccag aaccactgtt tgccatcttt gatttcaaag tgcactttaa gatcggcgat
     7801 ttcaaggagg acttttcttc cttcagttac agcattcata acagttcctc caccggttta
     7861 aagcaagcac gcagacggcc aggcgtaaac tcttccagcg gcggtgcgct actacaaatt
     7921 tccatcgcat gtggacaacg tggctggaac gggcaacctt tcggtaatcg cagcaagttt
     7981 ggcggattac ccgggatggt caacattgtt tcaccttccg catcaagacg cggcaccgcg
     8041 ttgagcaaac cgatagaata aggatgaacg ggttgataaa agacatcgcg cgcattgcca
     8101 tattccatcg tgcgcccggc gtacattacc agcactttgt cacagatccc cgccaccacg
     8161 ccaagatcgt gggtgatcat aatgatggcg gtattaaatt cccgcttcag ttcatttaat
     8221 agcgtcatga tctgcgcctg tacggtgacg tccagcgcag tagtgggttc atccgcaatc
     8281 agtagcttag gtcgacatag caatgccatc gcaatcatga ctcgctgacg catgccgcca
     8341 gaaaattcgt gcgggtacat tttcatgcgt ttacgcgctt ccggcatttt taccgcatcg
     8401 agcatccgca ccgactcttc aaacgcctca gctttgctca tgttcttatg cagcatcagc
     8461 acttccatta actgctcacc gacgcgcata tagggattca acgaagtcat tgggtcctga
     8521 aaaatcattg agatttgttc agcgcgcagt ttattgagtt catgctctgg caaattgagg
     8581 atttcacgcc cattgaaggt tgccgatccg ccaatacgtc cgttggcagc caacaggccc
     8641 atcaacgcaa atgcagtttg cgatttaccc gaaccagact cacctacaat acccagcgtt
     8701 tctccggcac gtagggaaaa attcaaatca ttgaccgccg tgacgtcgcc gtccggggta
     8761 ctaaaggtga cacgcaaatc tttcacgttc agcagtgcgt cagcctgttg ttgtgcgagc
     8821 ggcacagttg cagtttcaat tacgctcatg gctgcactcc ttaacgatct ttcgggtcga
     8881 gggcatcacg caagccatcg ccgataaagt tgaaacaaaa cagcgtcacc acgaggaatc
     8941 ccgctgggaa caacaataac catggagaga cttccatcga gttcgcgcca tcactcagca
     9001 atgcacccca gctgcttaac ggctcttgcg tacccaaccc caggaagcta aggaaagatt
     9061 caaagaggat catgctgggc accagaagtg atgcgtagac caccaccaca ccgagtacat
     9121 tcggcacaat gtggcgaata acaatacccg atgtcgatac accgccaact tgtgccgcct
     9181 caataaactc tttgcgcttc agactcaggg tttgcccacg cacaatacga gccatatcca
     9241 gccaggaaac catgcctatc gccacgaaaa tcagcaggat gttttgaccg aaaaaggtca
     9301 ccagcaaaat gacgaagaac atgaatggga aggagttgag gatttccagc agacgcatca
     9361 ttaccgaatc cactttaccg cccagataac cggaaagcga accataaagt gtcccgacga
     9421 ccacagccac cagtgccgca gcaacaccga ccatgagtga gatacgcccg ccaatcgcaa
     9481 cgcgcacaag caggtcgcga ccggatgagt cagtaccaaa gtagtgaccg gactccatat
     9541 ccggggcgct ggacatcatc gcccagtcag tatcgtcata ggcaaactgg gaaagcatcg
     9601 gtgccaggat tacaaataac gcgatcagca ccagcactat cagactggca accgccgcac
     9661 ggttatgcat aaaacgtcga cgtgcgtcct gccacaagct gcgcccttcg acctccagct
     9721 tttcactgaa attttccagc gtctcgctgt ttttcttact taacatcatc gcgagctcca
     9781 gtatcagtaa cggattttcg ggtcgataac cgcatatagc acatcgacaa tggcattaaa
     9841 caaaatggtt aaagcaccaa ccaggatggt caggcttaac actaaggaat agtcacggtt
     9901 caatgcgcca ttaacgaaca gttgtccaat ccccggcaaa ccataaatgg tttcgataac
     9961 catagaaccg gtaataatgc cgacaaatgc ggggcccata taagagagca cgggtaacag
    10021 agcaggtttt aatgcgtgac gtaaaatgat ccgccgcata ggtaaccctt tcgcccgggc
    10081 agtacgaata aaattggagt gtaatacttc aatcatagag ccacgggtaa tacgcgcaat
    10141 actggcgata taagcgagtg acaacgccac catcggcaat atcatgaatt taagcgcccc
    10201 accattccag ccaccgcccg gcagccaatg caaaatgatc gcaaatatca tgactaataa
    10261 tggtgcaacc acaaaactgg ggataacaac cccggtcatt gccagcccca tcacggtata
    10321 gtcccatttg gtgttttgtt ttaatgcggc aataacgcca gcactaacac ccagtattac
    10381 cgcaaggaaa aatgctgcgg ctcccagttt ggcagaaacg ggaaaactgg atgcaaccaa
    10441 gtcattgacc gaataatctt tatatttaaa cgatggaccg aaatcaccgt gcgccagttg
    10501 tttcaggtag ctgaaatact gtgtcatgat cggatcatta agatgatatt tcgcttcgat
    10561 attggccatc acttctggcg gtaaagtacg ttcgccggta aaagggcttc ccggcgcgag
    10621 gcgcatcata aagaacgaaa tagtaataag aataaatagc gtcggaatcg cttccagaca
    10681 gcgacgtaga ataaatttta acattgccct accttctggc ctgtgccttc tgtaatgcga
    10741 taaaaatcag acaccgtgga gcaggacact cctgccccac gtattgccat tagtgcttca
    10801 caatgtacat attccgggta taggtattat ccagcggatc tttgccggta tagccaccaa
    10861 cccacggttt caccagacgc gcattcacgt agtaataaac aggaacaatg gccgaatcct
    10921 tatccagctg ttgttctgct ttagtgtaca gagctgtgcg ctgcgcctcg tcagtcactt
    10981 tcagcgtttc cgccataatg ctgtcaaagg ccgggctctt ataatgcgcg gtattcatcg
    11041 agctgttcga aagcatggtg ttcaggaagg aagttggttc gttgtagtca gcacaccagc
    11101 ctgcacgggc cacatcaaaa gtaccctggt gacgggtgtc gaggaacgtt ttccactcct
    11161 ggttaaccag tttgacgttt acaccaatgt ttttcttcca caatgaagag gcagcaatcg
    11221 ccagcttttt atgcagatcg gaggtgttat acaacaggtt gatggtcaac ggtttgtctg
    11281 cggtataacc cgcttcagcc agcagttttt tcgcttcttc gttacgtttt tcctggctcc
    11341 agccaaacca ttccggctga gtcaattttg cgccatcagt atacggtgga gtgtaaccat
    11401 aggcgggcat gtcgccctgc gctttcactt tattaacaat gatatcgcgg tccataccta
    11461 gtttcagtgc ggtacgcaca cgcacatcgt tgaatggcgg tttctggttg ttaatttcgt
    11521 aatagtaagt gcacaggtat ggatcaacgt gaacttcgtc cgggatctct tttttcagct
    11581 tctggaacaa ttcgatcggc atgttgttat aagtcatgtc gatttcacca ctacggtagc
    11641 ggttgacatc ggtaacttca gaagcaatag gcaaataggt tacctggtta ataacggttt
    11701 tcgcgttgtt ccagtaggtc gggctgcgtt caagaacgat tcgttcgttt acgacccaat
    11761 cttttaaggt ataggcaccg ttggtgacga tattaccagg ctgggtccat ttttcgccga
    11821 atttctcgat agcggctttt ggcaccggtg aagttgatgg gtgaacaagt aatttataga
    11881 agtacggaac gggttcactt aaggtgactt ctaatgtgtg atcatcaata gctttcacgc
    11941 cgagatcggt aatcggtttt ttcccttcaa gaatttcatc aataccggcg atatgcccat
    12001 attgcagata actggcatac ggagaagcag tgttcggatc aacagaacgt tgccagctat
    12061 acacaaagtc ttgtgctgtg actggcgtgc catcagacca tttcgcatct ttacgcaaat
    12121 ggaaggtcca gactttcgcg tctttattat cccaggattc agcgacgcca ggtgctggat
    12181 gaccgttaag atcgctgacc agtaagcctt caaacaggtc tcggctgata ttagactccg
    12241 gaacaccttc aattttgtgc ggatctaatg actgaacttc tgaaccattg ttacgtacca
    12301 gtgtttgttt ttccgccagt gtgacgcctg cgggtacatc agctgccagc gcgacattcc
    12361 ctgccattag cgcagccaga acgccagctg ctactaaact tctcttggtg atgttggtca
    12421 ttgttttttg gactccctca ttataattac tggtattaac cagcatgtgt aatcccggat
    12481 tgggaccctg cacaggctcg gagactttct gctgtcaggt tactttatcg ttacgtatta
    12541 ctatcaccga ctttatttat tctggtgatt cagacgatca ccttatgtcg attcgtactg
    12601 tgtctcccct gtcgagacac attattcttt cgcgacgctc tttaattgtt aattattctc
    12661 attaccaata aataatcgtc tggcggttgc ccggaaagta ccaaatgcgg ttcttactcg
    12721 ccaatacatt ttgcaaaatt gttaagcaat tctcttttat tgaccgtatc agaatctata
    12781 gcttgcatcc atgccttaac aaacatcttt caattcaatc aaatagagat tggctaagca
    12841 tgtctatctt ttctgattac agttccagta gtgaaatgca caataattta acaattgatt
    12901 attatttagc actttcctct accaaaggca gtggaattac taatatcatt tcaattattc
    12961 ttcagcaagc ccaggattat gatgtggcga aaataacatg atggtgagga gaagcagtta
    13021 aggaaatcct tataactaac tgtaatttga gaagaatatt tttacctatt ccttttgctt
    13081 ttgataactg cgcgggaaca aagtaaaaat aatgacgaag taaacggagc atggcagctc
    13141 cgtttcattt gaaaggaatt aattaagcag gccggggaaa atccccttaa taccagtaac
    13201 gataaattca atccccaatg ccatcagcaa tagccccata atacgcgtaa tcacgttgat
    13261 gccggtctgg cgtaaaaccc gtaccagcca cggtgccatg cggaacaatc cccagcaaca
    13321 taaagcgaac aatgcaatag ccacaaagaa accaaacaga tagctaatgc tgtgataacg
    13381 cgtaccccag acgatggtag aactgatcgc ccctggcccc gccatcaacg gcaacgccaa
    13441 tggcaccaca ccaatgcttt cacgtaccgc ggtttctgat ttttcttgct tgttctgttt
    13501 atcctcgcca agcttgccgc tgatcatcga catcgctatc gtcaccacca ggatgccccc
    13561 ggcgatacgg aacgaatcaa ttgatatacc aaaaagttgc agaatcgtgt cgccgagaaa
    13621 aagcgagatc cacaagataa tggccacaga caggttggct gtaaggttag ttttgtttcg
    13681 cgctgccgct gtctgataac tggtcatgct gataaagacg ggaataatcc ctaccgggtt
    13741 gaccagcgca aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa aaaaggtctg
    13801 aatcacggtt agctccgaag caaaagccgg ataatgttag ccataaataa ggttgaaaag
    13861 acgcgctgac aatacgcctt ttgacagcat ttttcacctc ctaactactt aaaattgcta
    13921 tcattcgtta ttgttatcta gttgtgcaaa acatgctaat gtagccacca aatcatacta
    13981 caatttatta actgttagct ataatggcga aaagcgatgc tgaaaggtgt cagctttgca
    14041 aaaatttgat ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc
    14101 tggaagtgac gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt
    14161 gagtgtgagc gcgagtaagc ttttgatttt cataggttaa gcaaatcatc accgcactga
    14221 ctatactctc gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca
    14281 ttatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc
    14341 agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc
    14401 tggctgctgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg
    14461 gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct
    14521 ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta
    14581 tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg
    14641 ctatcttcaa atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc
    14701 cgcgtgcaaa agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg
    14761 ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg
    14821 cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta
    14881 aagccgcata cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg
    14941 ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct
    15001 tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg
    15061 acgctgtacg tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga
    15121 aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc
    15181 cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga
    15241 tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga
    15301 ctctggcaat gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg
    15361 ttgctatggg cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg
    15421 ctcgcgtttc ttacttcggt cagaaaatga aaacggctcg tatcctgatt aacaccccag
    15481 cgtctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg
    15541 gttgtggttc ttggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca
    15601 acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta
    15661 tctacttccg ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca
    15721 aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca
    15781 cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc
    15841 cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga
    15901 ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg
    15961 aacatccgga aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta
    16021 tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg
    16081 gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat
    16141 atccgctggc agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta
    16201 tggacatgcc gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg
    16261 aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga
    16321 aactgctgaa agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc
    16381 gtgaacgtgt tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg
    16441 gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg
    16501 caaacgccct gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc
    16561 agactgcatt cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg
    16621 accacttggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg
    16681 catggctgga aacgctgaag gctgaactgg gtattccgaa atctatccgt gaagctggcg
    16741 ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc
    16801 agtgcaccgg cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg
    16861 atacctacta cggtcgtgat tatgtagaag gtgaaactgc agcgaagaaa gaagctgctc
    16921 cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca
    16981 taaacggccc cttctgggca atgccgatca gttaaggatt agttgaccga tccttaaact
    17041 gaggcactat aacggcttcc acaacaggga gccgttttct tatgccactt ctcaatgatc
    17101 tgctcgattt cagtgaccat ccgcttatgc ctccgccctc tgcacaacta tttgcagaac
    17161 accttcccac cgagtggata caacactgcc tgacgctttc tgctcatgcg accgttcgcc
    17221 gccgtcgttt accgggggac atggttatct ggatggtggt gcaatgagcc aattaccgat
    17281 gttgttcgcc gtctgaacct gagcgcggat ggcgaagcgg ggatgaacct gctggcccgc
    17341 agcgctgtca cccaggcgcg tcagcgcgtg ggggccgccc cagtggaatg gctcttccgc
    17401 cagaccgcac agacaggggc gcggaacgtt acctgaagga tgactggcac ggcctgcaac
    17461 tttttgccat tgatggcgca cagttcagga cacctgataa acccgagctg cgtgaatatt
    17521 atgggtctgc caacacatcc actaagcggc agaacgccta cccggttatg cgtctggtgg
    17581 ccttaatgaa cctgggaagt cacattctgc tgaatgccgt gaccgcacct taccggcaga
    17641 gcgaaactgt gctggcccac tccatgctcg ccaccatccc ggataactct attacgttgt
    17701 ttgataaact tttttacagc gaagacctgc tgctgacgct gaaccagaag ggttgtaacc
    17761 gccactggct actgcctgcg tggaagaata tcgcatcaga aatgatagag ctgggaaaca
    17821 cggcgtcacc agggaccatc ccgaaacgac tggagcatct tcggggtgct ctggaagtgg
    17881 tgttcataac aaaacgcccc cgaccatcaa ggccgaggtc ggttaagata tcaaaaaccc
    17941 gttatcctgt gaaacatagt gccgctcctc ttaagtgaac ggcattgccc ttctggggcc
    18001 gtattttttg tttacccaaa gcaacttttc cataaaccga cagcattagc cttcatcata
    18061 tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa
    18121 cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt
    18181 atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
    18241 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
    18301 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
    18361 caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
    18421 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
    18481 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
    18541 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
    18601 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac
    18661 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
    18721 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
    18781 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
    18841 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
    18901 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
    18961 agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg agctatcatt
    19021 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
    19081 acctgttgcg caagtaatag ccctctgttg acctccagga gatagtgcaa tactaagtcc
    19141 atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
    19201 acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
    19261 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata
    19321 tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga
    19381 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
    19441 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
    19501 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
    19561 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
    19621 ctgctgctga agttgaagag cgcactcgta aactgcagca atatcgcgaa atgctgatcg
    19681 ctgacggtat tgacccgaac gaactgctga atagccttgc ttccgttaaa tctggcacca
    19741 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
    19801 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
    19861 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
    19921 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
    19981 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
    20041 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
    20101 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
    20161 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
    20221 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
    20281 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
    20341 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
    20401 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
    20461 ccgtttcatc aaagcggcgg atcatctctg ccaggttatc ctgtgacaaa tcggattcat
    20521 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
    20581 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
    20641 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
    20701 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
    20761 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
    20821 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
    20881 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
    20941 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
    21001 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gttagcataa
    21061 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
    21121 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
    21181 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
    21241 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
    21301 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
    21361 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
    21421 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
    21481 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
    21541 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
    21601 gcaccaggcc gggtttgtcc gcggcaacca attgacgata attaacccgg caatgggaaa
    21661 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
    21721 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
    21781 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
    21841 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcgg
    21901 tggcagatat caccagaacc ggtgtctggt cacctctgtt gcgtatatgt tccagcagtt
    21961 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
    22021 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
    22081 aaaaccacga atccagaagc gagcgaaata ccggctcatc ttcaacaatg agaatctgtt
    22141 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtgacatg
    22201 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
    22261 ttggtgcgta ccaacggcaa aagttcgtcc attttctttt ccactgccag ctgtccggcc
    22321 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
    22381 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccagcacc
    22441 tggatagaag tggtcataat ctctgttgcc gttggcgctg tcaccgcccg acgtgtcgtt
    22501 atgctgccta acctttcttt cagacgcgca tgccacggca gagaatcacc attctcgcta
    22561 ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc
    22621 aggtcaaccg ctatcacaat atcagccccc aatgcacgcg tgagggaaat aggaattggg
    22681 ttaacgactg ctccatcaac cagccagtag ccgttatgtg caacaggtgc catgagtcct
    22741 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
    22801 cgtcccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
    22861 gtttccggca ttatttcgcg gtattgattg aagacacgct cgccgcgcag taacccaccg
    22921 cgctgccagg agagatccat caggcgtaaa acatcccaat aactgaaaga ggtcacccaa
    22981 tcttccagcg cagataatcg atcgcatgca taggcagcgc ccaccagcga accaattgaa
    23041 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
    23101 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
    23161 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
    23221 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
    23281 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
    23341 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
    23401 caagacgcag attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
    23461 gccgaattga tggccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
    23521 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
    23581 ggtaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
    23641 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
    23701 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
    23761 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
    23821 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
    23881 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
    23941 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
    24001 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
    24061 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggcctgg atgtcgaaat
    24121 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
    24181 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
    24241 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
    24301 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
    24361 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
    24421 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
    24481 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
    24541 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggca cagcgcgtct ttgtttacgg
    24601 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
    24661 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
    24721 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
    24781 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
    24841 aggttcgaat ccttccccca ccaccatcac ttttcaaaag tccgtaaact ctcaaacgaa
    24901 tccgcaatta aatattctgc ccaagcgggg aaggatgaga agcttcgacc aaggttcgac
    24961 ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa gcgcgcagcg
    25021 ctgagtaatc cttcccccac caccatcact ttcaaaagtc cgtgaactct caaacgaatc
    25081 cgcgattaaa tattctgccc atgcggggaa ggatgagaag cctcgaccaa ggttcgactc
    25141 gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc gcgcagcgtt
    25201 gagtaatcct tcccccacca ccatcacttc cttaaataat caaaacctgt tactccgagc
    25261 taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct gtaggtcgga
    25321 taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac cccgccgaag
    25381 cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg gtacttacgt
    25441 gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg gaacagcagg
    25501 aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc atcgaggtgt
    25561 tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag tttcatcatc
    25621 tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag cgcgcactgg
    25681 ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg
    25741 ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac gccgctggca
    25801 ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc
    25861 cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat accgatatgg
    25921 aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc
    25981 caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct
    26041 atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg
    26101 tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga
    26161 ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct tcccagacag
    26221 catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc gccactttgt
    26281 cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg ccataacggc
    26341 tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata tctttcaaac
    26401 cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc ggcagatggt
    26461 ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca
    26521 ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg
    26581 tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc ggatccatcg
    26641 ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac agatttttcg
    26701 acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt
    26761 caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt tgctggtcac
    26821 atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg aaccgtggca
    26881 gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc gtgccagttc
    26941 acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta
    27001 catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt
    27061 accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc aacatacgtt tcagtgcgcg
    27121 cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta ccggaatacg
    27181 caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt ctgctgcaga
    27241 ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat attctggatg
    27301 cagcggcagc gccagtttcc attccattgc cattttataa accggcgact gctgtgcggc
    27361 ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt
    27421 cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct cggtgctggc
    27481 tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac
    27541 acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc agaagatgca
    27601 cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc atccggtgat
    27661 gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat cttcttcacg
    27721 cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca
    27781 caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg ccttctggat
    27841 gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt catcttccca
    27901 gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac gcggacgggc
    27961 aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa
    28021 cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt tacccagcaa
    28081 catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga tccagccgcc
    28141 tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct tggtttccac
    28201 gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtaac
    28261 tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga cttgtgaacg
    28321 aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg
    28381 ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt gccgtagtag
    28441 ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata
    28501 cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc aggcaggtta
    28561 acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca
    28621 accgcacggg cagtcagagc accgttgctg ttgaagactt caatccagtc gttatcggcg
    28681 atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa
    28741 gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggcgtc
    28801 aggaagttga gcgctttttc cgggttgcca ttggatttct ggcccatcac ttctttcacc
    28861 gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccat
    28921 tggtgatcct gatacagctg ctgacggcca gagagcgtac gccacgggat cagctcgtga
    28981 acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt caaggccaga ccaggtcggg
    29041 ctggagatga ttttgcgcgg ctgtgcctga atatcgcgga agcgaatctt ctcgtcttct
    29101 ttattcagcg ccagatgcgt atggtcacga ccggtaaact cgctcagggc agcccaggct
    29161 ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc tgccgcatca
    29221 attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt gtagttgagc
    29281 ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc gccattaccg
    29341 attttctcca tcagcgggcc gatagaggta aagcgttcgt aagtcgccgg ataatcgcgc
    29401 tctacgacca taatgtgtgg cgcggtttta ccagggatca ggtcgcactc gccttttttc
    29461 cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg gataggcagc
    29521 gtgacgatgt cggtttcttt acccagatga ccgacgcaca cttcggagaa tttcttcgcg
    29581 atggctttgt aaatttccca gtcgcttttc gcttcccagg ccggatcgac cgccgcagac
    29641 agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc
    29701 gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgta
    29761 accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc ttccggcttc
    29821 acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc cagcaggtac
    29881 ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac
    29941 aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc
    30001 tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc cgcttcgcca
    30061 gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc catgcgttcc
    30121 gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc catcggtgac
    30181 agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt atagaaataa
    30241 gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc
    30301 cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc gcccccgctc
    30361 tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt catatcgagg
    30421 tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgttttatca
    30481 gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac gccggtaatc
    30541 tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc acagttaacg
    30601 tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac ggtggtcacc
    30661 agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg
    30721 ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct
    30781 acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt ttcttcgcca
    30841 gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata
    30901 gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt ccattccgga
    30961 ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg acctgcagcg
    31021 tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta gcgacgcaca
    31081 tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat tacgtggccc
    31141 atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag atcgcacagt
    31201 ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtcccttt gtaacgcact
    31261 tcagtaaaga agtgagcatc cggggtacgc gtctgcggta cgtttgaccc ccaggcgatg
    31321 atgtagctgg agttgtacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt
    31381 tgcggagacg caggaggcaa gtcgcagtac cagtcgtaga agcttaagca agtaccgcca
    31441 atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc cggaattggc
    31501 gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg
    31561 atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc acgcgcctgc
    31621 ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg
    31681 ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat
    31741 ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg gcagccgcga
    31801 ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg ggtttcccag
    31861 gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca gtttaccccg
    31921 tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca
    31981 cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt ctgtttgaag
    32041 tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggccct
    32101 ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa
    32161 ttgataacga tcaagtgtga tggggatgaa aaataaagta aattcctaag ataccccttt
    32221 aggataatta acagttaaag cataacatgc tgtaaattaa gacgttttat acctgtagtt
    32281 atttaaagca tgaagatttt tatgcagatg ggtattaagg agtattcccc atgggtaaca
    32341 tattaatggc tcttaatgaa atccggcgaa taagccatat aacattagta tgttgtaact
    32401 aaataacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa atattttact
    32461 ctatataatt cggtttacag gaaggcggca agtgaatgaa tcccaggagc ttactaaagt
    32521 aagtgactgg ggtgaatgaa cgcagccgca gcacatgtaa cccgaagtat gacgagtaac
    32581 ggtttatttt ttagaatgcc gaccatatac cgcccaggta atcactacgc aggcgatata
    32641 gaaaatcaaa aataccttca ttgcgccgac tggcgaaccc gttaatgcca gcgagctacc
    32701 aaacgctttc gggataaaga agccaccaat agcgccaatc gcagagatga aacccagcgc
    32761 cgctgccgtg tcggttgccg cttcacgcat cgcacgttcg tcagaacccc cttctgcttt
    32821 cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag tggaaccact
    32881 acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca tgaagcttcc
    32941 gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg ccatcagaat
    33001 aaagttcacc agcgtgacac gagttccgcc cagacggtca gataatgcac cacctgcaga
    33061 acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct gaacatccgg
    33121 gaactgcgtt tttgacagca ttgcaaagcc cgcggagaag ccgatgaagg agccgaaggt
    33181 tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg gcaactgctc
    33241 cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg caatagtgaa
    33301 gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct cagtcccatc
    33361 cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata ccaccagcgg
    33421 agcaaccaac tgcatgacgc tgacgcccat gttgcccaga ccaccattca gacccagcgc
    33481 gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg aggcgaagtt
    33541 cgcgccagca aagccgcata gcagagagat gatgatgaag acgctataag gcgtggaggt
    33601 atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg tgctgaacgc
    33661 cgtccagcga cgaccaccga agataggaac cataaaggag tatggaacac gtaataacgc
    33721 gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa aattaaagcc
    33781 gactttcggt aggttcaccg caacagcgct gaacaacatc catacgcaaa acgccagcag
    33841 cagacaggga acggaaatcc acaggttgcg gctggcaata cgttgaccgc gttgttgcca
    33901 gaacgcagga tcttccggtc gccaatctgt aatgacagct ccagtagccc tttcgggggc
    33961 ggatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc ttagtgtctg
    34021 tagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat cattgatatt
    34081 tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt tcacggtgct
    34141 gtagaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata aggagtaact
    34201 ctttccaggt atgggtatac ttcagccaat agccgagaat actgccattc cagaatgtat
    34261 cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaagaggtt tacatgctta
    34321 aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg ttgctttcta
    34381 ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc gttcagggca
    34441 gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt ctgttggcag
    34501 cagtgccatt aagcgagaaa gacaagccct taattaaaga gatggaacaa acggcattta
    34561 gcgccgagtt gactcgagca gcagaacgag acggacaact ggcgcaatta cagggtttac
    34621 aagattactg gcgtaatgaa ctgatccctg cgttgatgcg tgcacaaaac cgagaaacgg
    34681 tgtcagcgga tgtcagccag tttgttgccg ggcttgatca actggtatct ggttttgacc
    34741 gcaccacgga aatgcgcatc gagacagtgg tactggtcca tcgggtaatg gcggtattta
    34801 tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta caaccgtggc
    34861 ggcaactgct ggcaatggcg agtgccgtca gtcatcgcga ttttacccaa cgcgcaaaca
    34921 tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt gaacaatatg tctgcagaac
    34981 tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc gggctggagc
    35041 ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat tcccaagccc
    35101 cgctgtgtga acgcctgtca cctgtactca acggcttaca gaatttaacc ctgctacgtg
    35161 atatcgaact gcgggtttat gatactgatg atgaagagaa tcatcaggag tttacctgcc
    35221 aaccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc gtattacccg
    35281 ttggtgatcg cggcacaacc ctgaagtggc ggctggctga ctctcatacg cagtacggta
    35341 ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa caactggtgg
    35401 ataccctggt tgaacaactc accgccacgc tggcgctgga tcgccatcag gaacgtcagc
    35461 aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat gattctattg
    35521 cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag ggcgatgcgc
    35581 tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat gcatcctggg
    35641 cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct ggattacgtc
    35701 cggcgctgga ggcgagttgc gaagagtaca gcgccaaatt tggcttcccg gtgaagctgg
    35761 attatcaatt gccgcctcgc ctggtgcctt cgcatcaggc aatccacttg ttgcaaattg
    35821 cccgtgaggc attaagtaac gccctcaaac actcgcaagc gagtgaagtc gtggtgacgg
    35881 tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc ggcgtgcctg
    35941 aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt gcgcaaagtt
    36001 tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg gtggtcacct
    36061 ttattcccga aatactttca cagacgtcca aggagatacc catgagtaat caggaaccgg
    36121 ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa cagcttatca
    36181 gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag ggtattgaac
    36241 tggcggagtc tcttgatccc gatctgatcc tgttagatct caatatgccc ggcatgaacg
    36301 gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt gtggtattca
    36361 gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg gatggttatc
    36421 tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca gctgctggcg
    36481 aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc gctaaccgtg
    36541 ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt ctcaagctga
    36601 ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc gaaagcacag
    36661 taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc gtggaagcag
    36721 cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag ttcgtcgttt
    36781 gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga gacacgctgc
    36841 ccctggttat cttccaccac caccgacaat cgccagtgat tgcttgcccc ttccccgttc
    36901 tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc gcccggcgtc
    36961 aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta acgactgcgg
    37021 atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg tgtcgccaga
    37081 aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt tcgctgcgga
    37141 ttgtcatagc gactaccacg taacgactga ctttcggcaa cctcacccgc agaaagttgt
    37201 tttttgagcg gtacaccaaa gcggtaatta agattcagcc cgaggttatt ttgattctcg
    37261 ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg ggtgtaattt
    37321 aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa caaatcaaca
    37381 cgatcaccaa aatactgttc tacgctgaca ctggtattga ggtgttgata gaacggcatg
    37441 cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt ggctgtctgt
    37501 tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata ttcgccccac
    37561 gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc ataaaaagtg
    37621 ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac attgctcacc
    37681 aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg atcattatct
    37741 tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt atccactttg
    37801 acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg attaacctgt
    37861 tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc gccagtatcc
    37921 agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc aaagtgtttt
    37981 tccccatcat gattttcggg agccatgcct aaatccggca gaccatcatt gttattatca
    38041 aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc gcctgctaca
    38101 agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac gtcggtaact
    38161 tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa cttttgtcgt
    38221 attttctcgc gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga
    38281 agcagtaaga tagaagatga atgtctaatt ccctgaatac aggagtaaat aatgcaaaaa
    38341 atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg
    38401 ctagccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctgatgtct
    38461 gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa
    38521 atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac
    38581 gggcgcggga ttagtacact tcctctgatt gatggggtgg aaatcggtac tcttgtggaa
    38641 ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt gagggggcgt
    38701 ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac acaccatccg
    38761 gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct
    38821 gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg ttggttccca
    38881 gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg tcagattcat
    38941 attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcat ccatcatcaa
    39001 gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc ttccacaaca
    39061 gggttagttc ctgctccagc gtctcttctg gcagtcgata ggcgacgcct gtagtgcgtc
    39121 cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca
    39181 ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcacgac tcggtaaact
    39241 ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt ccctcatcag
    39301 gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ctctgctgac cataagagtg
    39361 attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta
    39421 tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac
    39481 atttttactg ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc
    39541 aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat
    39601 ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgcg
    39661 gtttcttcgc gactggcgtc atcacgccga tcgtctttat ctttatattg atcccatgcg
    39721 ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg ctttacgctt
    39781 tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat
    39841 taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg tcagactgtt
    39901 cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat gtcccaatga
    39961 gtaaaaaatc gacagtgggg taaaaattcg taaaatgtta ccgatatcca gacactttct
    40021 gttatttttc ctacctattt tttacatttc cgataaattc atctgcaaag ggataaccat
    40081 aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc
    40141 ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac cagtggtcat
    40201 tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg ttgtccgtca
    40261 tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc ttagcctttc
    40321 agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc
    40381 aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg
    40441 cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat
    40501 caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc
    40561 tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc
    40621 aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga
    40681 gcacgaagat gacagtgatg acgatgaccc gcatcacggt aaaccgtctg cccatagcag
    40741 cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag tcaccaaaat
    40801 gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg cctttactgg
    40861 cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt
    40921 gaacaaccag gtccagcgcg cgatgaatct gttctttggt tcagtgttag caaccatttc
    40981 gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tacagtttgc
    41041 acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc atatctcctt
    41101 ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta
    41161 tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat cgggggagat
    41221 aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt taatcagaat
    41281 tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa agccggaatg
    41341 tactctttat tgcaaacatt tcagggatga agtgaagatg aattgcgacg tagtatccac
    41401 accaagccgg cgttatccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt
    41461 ggttgct
//
LOCUS       DAZUFB010000033        40702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZUFB010000033 DAZUFB010000000
VERSION     DAZUFB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.033"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ACHY3M_001994"
     CDS             complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ACHY3M_001994"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFS4316915.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1849..3498
                     /locus_tag="ACHY3M_001995"
     CDS             1849..3498
                     /locus_tag="ACHY3M_001995"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS4316916.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESSREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3549..4151)
                     /locus_tag="ACHY3M_001996"
     CDS             complement(3549..4151)
                     /locus_tag="ACHY3M_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4316917.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4671..5570
                     /locus_tag="ACHY3M_001997"
     CDS             4671..5570
                     /locus_tag="ACHY3M_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4316918.1"
                     /translation="MDKIHAMQLFIKVAELESFSHAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5619..6632
                     /gene="yhjD"
                     /locus_tag="ACHY3M_001998"
     CDS             5619..6632
                     /gene="yhjD"
                     /locus_tag="ACHY3M_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFS4316919.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7043..8365
                     /locus_tag="ACHY3M_001999"
     CDS             7043..8365
                     /locus_tag="ACHY3M_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4316920.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGSLLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8599..10659)
                     /locus_tag="ACHY3M_002000"
     CDS             complement(8599..10659)
                     /locus_tag="ACHY3M_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS4316921.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHY3M_002001"
     CDS             complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHY3M_002001"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFS4316922.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHY3M_002002"
     CDS             11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHY3M_002002"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFS4316923.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12753..14249)
                     /locus_tag="ACHY3M_002003"
     CDS             complement(12753..14249)
                     /locus_tag="ACHY3M_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4316924.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHY3M_002004"
     CDS             complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHY3M_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFS4316925.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHY3M_002005"
     CDS             complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHY3M_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFS4316926.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18009..21482)
                     /gene="bcsC"
                     /locus_tag="ACHY3M_002006"
     CDS             complement(18009..21482)
                     /gene="bcsC"
                     /locus_tag="ACHY3M_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFS4316927.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21464..22570)
                     /gene="bcsZ"
                     /locus_tag="ACHY3M_002007"
     CDS             complement(21464..22570)
                     /gene="bcsZ"
                     /locus_tag="ACHY3M_002007"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFS4316928.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22577..24916)
                     /gene="bcsB"
                     /locus_tag="ACHY3M_002008"
                     /pseudo
     CDS             complement(22577..24916)
                     /gene="bcsB"
                     /locus_tag="ACHY3M_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            complement(24927..27545)
                     /gene="bcsA"
                     /locus_tag="ACHY3M_002009"
     CDS             complement(24927..27545)
                     /gene="bcsA"
                     /locus_tag="ACHY3M_002009"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFS4316929.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27542..28294)
                     /gene="bcsQ"
                     /locus_tag="ACHY3M_002010"
     CDS             complement(27542..28294)
                     /gene="bcsQ"
                     /locus_tag="ACHY3M_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFS4316930.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28306..28494)
                     /gene="bcsR"
                     /locus_tag="ACHY3M_002011"
     CDS             complement(28306..28494)
                     /gene="bcsR"
                     /locus_tag="ACHY3M_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFS4316931.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28767..30338
                     /gene="bcsE"
                     /locus_tag="ACHY3M_002012"
     CDS             28767..30338
                     /gene="bcsE"
                     /locus_tag="ACHY3M_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFS4316932.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELMQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30335..30526
                     /gene="bcsF"
                     /locus_tag="ACHY3M_002013"
     CDS             30335..30526
                     /gene="bcsF"
                     /locus_tag="ACHY3M_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFS4316933.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30523..32202
                     /gene="bcsG"
                     /locus_tag="ACHY3M_002014"
     CDS             30523..32202
                     /gene="bcsG"
                     /locus_tag="ACHY3M_002014"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFS4316934.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELIDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32289..32396)
                     /locus_tag="ACHY3M_002015"
     CDS             complement(32289..32396)
                     /locus_tag="ACHY3M_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4316935.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32772..32879)
                     /locus_tag="ACHY3M_002016"
     CDS             complement(32772..32879)
                     /locus_tag="ACHY3M_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4316936.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            33355..34626
                     /locus_tag="ACHY3M_002017"
     CDS             33355..34626
                     /locus_tag="ACHY3M_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFS4316937.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(34656..35660)
                     /gene="dppF"
                     /locus_tag="ACHY3M_002018"
     CDS             complement(34656..35660)
                     /gene="dppF"
                     /locus_tag="ACHY3M_002018"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFS4316938.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(35657..36640)
                     /gene="dppD"
                     /locus_tag="ACHY3M_002019"
     CDS             complement(35657..36640)
                     /gene="dppD"
                     /locus_tag="ACHY3M_002019"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFS4316939.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(36651..37553)
                     /gene="dppC"
                     /locus_tag="ACHY3M_002020"
     CDS             complement(36651..37553)
                     /gene="dppC"
                     /locus_tag="ACHY3M_002020"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFS4316940.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(37563..38582)
                     /gene="dppB"
                     /locus_tag="ACHY3M_002021"
     CDS             complement(37563..38582)
                     /gene="dppB"
                     /locus_tag="ACHY3M_002021"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFS4316941.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(38733..40340)
                     /gene="dppA"
                     /locus_tag="ACHY3M_002022"
     CDS             complement(38733..40340)
                     /gene="dppA"
                     /locus_tag="ACHY3M_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFS4316942.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDALIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         9491 a        11191 c        10277 g         9743 t
ORIGIN      
        1 cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct
       61 gcatatacgg cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat
      121 cctcctgcgg cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca
      181 gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg
      241 gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg
      301 ccgccgtaat ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc
      361 cgccgagaat aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc
      421 cttttttctg ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg
      481 aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac
      541 tgaaaccttg cggataaact tcgaggaact gcgctttaag ctgcggatct ttttccagct
      601 tagcaataat ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac
      661 ctgcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg
      721 agttaaatac cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt
      781 ttctgccatc gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat
      841 cagccgataa acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg
      901 taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat
      961 tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt
     1021 catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat
     1081 actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca
     1141 gtgccgcacg cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca
     1201 tcaactgttt cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc
     1261 aatagtcgca tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag
     1321 atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata
     1381 accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt
     1441 tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg
     1501 caaacgtctc tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga
     1561 atcaatcaat tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc
     1621 aggaatggca tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca
     1681 acacgaaatc cgtattttta taaattccat tgatattagt gcgttatatt ttttgacaag
     1741 ttaataaacg tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct
     1801 taaagaaaag cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag
     1861 aaaattcaaa accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg
     1921 gacccgtatg aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt
     1981 gaagggctgc ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag
     2041 catgttcagt cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa
     2101 atggacccgc tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac
     2161 ttgcgcaagt ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta
     2221 tcggacccgc aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc
     2281 gaaccacagg atcacattcc gtggtcttct ctgctggcgc taccgcagtc atatattgtc
     2341 ccgggcggcc gttttagcga aacctactat tgggattcct atttcaccat gctggggctg
     2401 gcggaaagta gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc
     2461 gaaaactatg gtcacatccc caacggcaac cgcacctatt atttgagccg atcgcaacca
     2521 ccggtttttg cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc
     2581 tatctcgacc accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc
     2641 cctaatcagg cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttat
     2701 tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa
     2761 cattctggtc gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc ggcctcaggt
     2821 tgggattact cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc
     2881 cagttcatcc ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac
     2941 atctcggcgc tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc
     3001 cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac
     3061 gactggcgac gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc
     3121 ggcatggcga accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg
     3181 acacctggcg ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaat
     3241 ggctgggcac cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt
     3301 ctgggtgatg aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag
     3361 cacaaaatga tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc
     3421 gagtatccgt tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt
     3481 ttgtacggcg aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat
     3541 tatcctcttc aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc
     3601 agacggcgat acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgct
     3661 ctgccgatct ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg
     3721 gcgcttaacg atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc
     3781 agaaacatca tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa
     3841 tcacgaggca caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg
     3901 caatccgtcg ccgtgattaa caccttaacc tcaggaaact gcaccacggt tttttgcagc
     3961 aaccagtagc aaaactcacc atcctgatcg ccatcgagca taactaaggc ttcagggtaa
     4021 ctttccagct tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga
     4081 atacgctgct gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg
     4141 actatttgca taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg
     4201 tattacccgc gagaaaaaat aagaattcgc catttggcgg tgaccattct acagagatga
     4261 cgcgtagaaa atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg
     4321 agggcactat ttaaaacaat tttgaggatt tccttatatt ggtggttagt acgcatgcaa
     4381 ttaaaaatga aactccgcga ccacaagcaa aaataacaaa cggcaatgag acaaaaataa
     4441 gcacaaatag ccaacacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct
     4501 gtttaggggg attcaccttt ctcagaaagc tattccgccc ttttcctgct gagaaatcgc
     4561 cacattcgtc atgacaacat tgtgaaaccc ggcattagat gttagaaaaa acaataaaca
     4621 atgcgatatg cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa
     4681 ttcacgcaat gcagttgttc atcaaagtcg cggagctgga aagtttttcc cacgcggcgg
     4741 atttctttgc tttgccaaaa ggaagtgttt cgcgccagat acaggcactg gaacatcaac
     4801 ttggcaccca gcttctccag cgcaccacgc gacgggtcaa actcacgcca gaaggcatga
     4861 cctattatca acgagcaaaa gatgtgttga gtaatctcag cgaactggac ggtctgtttc
     4921 aacaggatgc caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga
     4981 aaagcctgtt actgccgcgc ctgtcggaat ttctctatct gcatccggga attgagctgg
     5041 aactgagtag ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac
     5101 gcactggcgc gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg
     5161 tcaactgtgc cagtccgcac tatctgacgc gctttggtta tccgcaaagc cccgatgatc
     5221 tgacttcaca cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg
     5281 aggttgccag cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca
     5341 gtagcgaaaa ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca
     5401 ttgccgtgcg cgaagccctg cgtgccgggc ggcttattga agtgttgcca ggctaccgtg
     5461 ccgagccgct ctccctttcg ctggtttacc cgcagcgtcg ggagctttcc cggcgtgtaa
     5521 acctgtttat gcagtggctg gctggcgtaa tgaaagagca cctggactga ccgactatac
     5581 tttttaagaa tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca
     5641 aacgtcccac ccaggatctg gagcacgagc cgattaaaca gttggataat agcgagaaag
     5701 gcggcaaagt tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc
     5761 aaccggttat tgcgcacctg attcgtgcga cagaacgctt taacgatcgg ctgggtaacc
     5821 agtttggtgc ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtgt
     5881 cgtttgccgc agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg
     5941 acaaaattct gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca
     6001 acaccgccgt tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcactttatt
     6061 ccggcatcaa ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct
     6121 gggaacgctc gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt
     6181 cactgattgg tttgttaatt gcgctgattg tgacgctgtc gatcacctcg gttgccggtt
     6241 cggcgcagca aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga
     6301 cgtggcggtt gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga
     6361 tcttctggcg gttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc
     6421 tggctgctat tggttttgaa gtgattaaaa tcgtgatgac ctacaccttg ccatcgctga
     6481 tgaaatcccc ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact
     6541 tcttcgcccg tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg
     6601 acccgcgaat gccagggaaa acgcagcctt aaaataggcc ggatgcggcg taaacgcctt
     6661 atccggcata cgattttatt cggcctggct ccccgtaggc cggataagat gcgccagcat
     6721 cgcatccggc tataatgcgc acataacctc ttgaaactct tccccagagc ctcttcagcc
     6781 atctatttgg gagcaaacaa tttcattcca actcataacc ccagcatata aatccagttg
     6841 gtaactttta tttaacctga aaccagtttt atccactatt tataaaatta tgtgaagcat
     6901 ttcatagaag aaaaatcact ggcctaaaca ttatcccctt tttgcctggt ttttgaccat
     6961 ttccgcgatt tgttacacat tgaaatatca cttttgctgt gcgtaatatg gctattcgtt
     7021 agccaaaaaa taagaaaaga ttatgcaagc aacagccaca acactcgacc acgagcaaga
     7081 atacacgccg atcaactcgc gtaataaagt ccttgtcgcc tctctcattg gcacggccat
     7141 tgagttcttc gacttttaca tttacgccac tgcggccgtt attgtgtttc cgcatatctt
     7201 cttcccgcag ggcgatccaa cggcagcaac gctacagtcg ctcgccacct tcgccatcgc
     7261 cttcgtcgcg cgccccattg gctctgccgt ttttggtcat tttggcgatc gcgttgggcg
     7321 taaagcgacg ctggtcgcct cgttgctaac gatggggatt tcgaccgtgg tgattggtct
     7381 gctgccgggc tatgccacga ttggtatttt cgccccgctg ctgctggcgc tggctcgatt
     7441 tggtcagggt ctgggcttag gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa
     7501 cgccccaccg cgcaaacgtg cactgtatgg ctccttccca cagctgggcg caccgattgg
     7561 cttcttcttt gccaacggca ctttcttgct gctttcctgg ctactgaccg acgagcagtt
     7621 tatgagctgg ggctggcgtg tgccatttat cttctcggcg gtgctggtca ttatcggcct
     7681 gtatgttcgc gtgtcgctgc atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa
     7741 acaggtgaag atcccgctgg gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg
     7801 tacgttcatt atgctggcaa cctatacgct gttttacatc atgacggtct actctatgac
     7861 cttcagtacc gccgccgcgc cagttgggct tggcctgccg cgtaacgaag tgttgtggat
     7921 gctgatgatg gcagttattg gttttggcgt gatggtgcca gtcgccggat tactggctga
     7981 tgcctttggt cgccgtaaaa gcatggtaat catcaccacg ctgatcatcc tgttcgcgct
     8041 gttcgccttt aacccgctgc tcggttctgg caacccgatt ctggtttttg ccttcctgct
     8101 gctggggtta agtctgatgg gactgacctt cgggccgatg ggctcgctgt taccagagct
     8161 gttcccgaca gaagtgcgtt acaccggagc atcgttctct tacaacgtag cgtcgattct
     8221 cggggcttcc gttgcaccat atatcgcagc ctggttgcag gctaactacg ggttaggtgc
     8281 ggtggggtta tatctggcgg cgatggctgg cttgacgtta atcgccctgc tgctgaccca
     8341 tgagacgcga catcagtcgt tgtaagtatc gtgtgggcag ccaccctcac cccagccctc
     8401 tccctgaaag ggagagggag aaaagcggag cgagatgtta tatcgcgaac ataacagccc
     8461 tcccccagaa agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc
     8521 ctcgcccctt tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc
     8581 aaacacaatc caacctcatc acttcttcat ctgcgacaat atcgtccgac actgattagc
     8641 ctccccttcg gaaggtgaga tcagtgccag taacgccgcc gccggtgtta ccagcgttgc
     8701 cagtgccgca gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg
     8761 atttttaaac gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaattccttt
     8821 actctccgga tcaatagtca aatccagctg ttcggaagca aaacttgccg tgccggtaac
     8881 gttaatcaac gcgttctcag tatcgaaagc aaagatctgc gggcgcgcca cgccgttggc
     8941 aatatccaga ttcgccgccg cgcagttcac ccgcacctca tcatcaccaa atatcgcacc
     9001 gacaatgtag ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc
     9061 gtcattcatc aacagtttca ggttgccgtt actgttgcct aaaagtgccg ccaccgagtt
     9121 accgctaccg cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc
     9181 cacatcgggc atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg
     9241 catcggcttt ttatcgcctt ccagatgaat attcgccgca atgctgccgc ccgccatgcc
     9301 aaatttcagc ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc
     9361 gctaatcggc aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc
     9421 cataacgtcc catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg
     9481 aacgcttttt tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga
     9541 gtcaacgcca attaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc
     9601 ttccagtttt ggtcgtggct ttccggtggt gtagaccaga gaaccgtgga tatcgctatc
     9661 gccaattcgc ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt
     9721 cgctaccagc cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag
     9781 ttcatagaga tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat
     9841 cttcattggg tcattcacga cgccatcaaa agcaacacgg gtgttaccag agcggaaatc
     9901 agcctgtacc ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc
     9961 cgtacccgtg agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc
    10021 gcccaccttt tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg
    10081 cttgcctaac ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatggc
    10141 gatccgccct tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc
    10201 attcgcgtct ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcgga
    10261 gaggcgaatc aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt
    10321 ggtcagcagc gccagcgggg ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac
    10381 ttcgggaata tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg
    10441 cacccagctg cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc
    10501 acggatagcg aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag
    10561 tcgattccag tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc
    10621 cactgaaatc gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt
    10681 cctgtgccag tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag
    10741 aaccgccgta ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc
    10801 gaatgcgggc gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcgacaat
    10861 caccccgcga caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc
    10921 cggcgactga cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac
    10981 ttcacttagt gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc
    11041 aaattcacac atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc
    11101 gaaacgcagc cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc
    11161 gatgagcgta gggccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc
    11221 ggctttttgc gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt
    11281 gatttcagta aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac
    11341 caccgttaat agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt
    11401 gtaagcacgc tcacactgca accaaaaacg ccgctcctgc aagctctcga tgcttgcttc
    11461 agggttgctt attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt
    11521 gatgctgccc ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc
    11581 cggggtaaat ttcctcactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa
    11641 ataatggaac actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct
    11701 aattttttac agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc
    11761 atgattgagc tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg
    11821 aacacttccg tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta
    11881 acggcgctgg gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac
    11941 gttgatactt ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa
    12001 accgacagca ccggcgagcg tacgttctac tactggcgga acgaagccgc cgccaaattc
    12061 tggctggaga gtgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc
    12121 tacctgagcg ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc
    12181 ctgctgcgcg aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg
    12241 cgcctgtggg ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg
    12301 gatatcgcct tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa
    12361 gacgtcattg cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg
    12421 gattcttgcc tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg
    12481 ccgaaagaaa aagtgatcga taccaccgca gctggcgact ctttcagcgc cggttatctg
    12541 gcggtacgtt tgacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca
    12601 agtaccgtta ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg
    12661 cagcatatgc aaaaggctgg ataaagtgtt acccctctat ccagctcatc cataaatacc
    12721 tgatggttgt gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact
    12781 tcaggatgta cgtcatccgt ggcgacagag gtggcggcag cagcagtcga tggggccatg
    12841 atttgatccc aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc
    12901 agcagtatta acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc
    12961 accgtcaggc tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg
    13021 acgacctgat tttgcaacga acgcatccgc tgacccatca gaatatcggt atcagcccgc
    13081 gcataggcgg caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac
    13141 tcttcttccg gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg
    13201 ttgctgttca gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac
    13261 agcacacggc agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc
    13321 tgaacatgcc agaacagcgc ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg
    13381 gctgattcac gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc
    13441 accgcgtcag tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga
    13501 gctggcgttt cacgtttgcc tttcagttcg ccaaacgttt tgtttatttg gtcgacaacc
    13561 gagcgcgcat ccacgtttcc caccaccagt agcgtcattg catccggggt gtaccatttc
    13621 tgatagaaat ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg
    13681 tgacctaaca aggttgaccc tttcagacga tagcgccacc agccctcttt agtatcggca
    13741 ggccaggttg ctaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg
    13801 gtcaatttgc cagtggcatt tgccagataa gagagcgctt ctttcagcaa atcgttacgg
    13861 ttattgggca aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc
    13921 gggcgtttag ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca
    13981 ccgctttgcg ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt
    14041 tcggcgagcg aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg
    14101 ggggtggtca gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga
    14161 tcaggctgga gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc
    14221 gctaaaagtc gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa
    14281 aaaaactgcc cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca
    14341 agaacatgaa aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt
    14401 caggcacagg ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa
    14461 gtgatgagat taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcac
    14521 atcgtccagt tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac
    14581 gccgttaccg accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccgag
    14641 gatcagcgcc agacccgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac
    14701 gataaagcca ctacccgtta cccctgccgc ccctttagaa gaaagcagca acacgattaa
    14761 cagcgtgatt tggtggacga tatccatctg actgttagtg gcctgggcga taaacaccgc
    14821 cgccattgtc aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac
    14881 cagccccacc accgatttgc ggcagccgag tttctccatc ttgtcgagca tacgcggcag
    14941 cgccgactcg gaagatgaag tccccagtac aatcagcagt tcttcacgga tgtagcggat
    15001 aaatttgaag atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa
    15061 caggatacag gtaatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac
    15121 gccgtattta ccgatggtaa acgccattgc cccgaacgca ccaataggcg ccagacgcat
    15181 gatcatattg atgatgccga agatgacctg cgagaaactt tcgatgacgt taaaaatcag
    15241 ttggcctttg ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagcac
    15301 ctgcagaatg ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat
    15361 gaaggcgaca atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc
    15421 aagcgttgcc ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag
    15481 accaataatc agcgcgatgg tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt
    15541 acgaccgacc gccttcatgc tttccatacc cgcaatgccc gttacgacgg tacaaaagat
    15601 gacaggagcg atgatcatct taatgagctt aacgaagccg tcgccaagcg gtttcatttg
    15661 ctcgcctatt tcaggataga aatggccaag gagaatacca atggctatcg ctgtcaggac
    15721 ctgaaagtaa aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca
    15781 ggtcttgtaa ggttatgggg tacctgcggc cttaaaataa cacccagaca acatcacaga
    15841 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    15901 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    15961 tcttcgaaga tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg
    16021 cccgcttttg ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    16081 aagttcaggc tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag
    16141 ccttcaacaa acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc
    16201 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    16261 ttgcgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    16321 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    16381 tccgccacca tatttgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc
    16441 ataatgccgc gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga
    16501 ccgacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc
    16561 caactgccat ccggttgctg gatacgcagt aacacttccg cactgaccag ttttccgctg
    16621 gtcatctcga cctgtggctg taaccaaata gcaaactgat gattttccag tgcattaagg
    16681 atatcgctct cttccgtcaa ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac
    16741 tgaatctgat tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    16801 agctgttcgg cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg
    16861 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacctaag
    16921 gtgattgcgt gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    16981 atctgcgcga ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc
    17041 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag
    17101 gtgatgatca tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc
    17161 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct
    17221 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt
    17281 tcatcgtcct gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg
    17341 atggcgttaa gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac
    17401 cagctgatgg cgacggtcag gataagcgac aaaagtaagt aaatagtcac taacgttgag
    17461 agggtgctca tcacgaactt atacatacgg aaggaatccg cctgtagtac cagataggca
    17521 attggctgcg ggtttgccgg acgttcgagc gagtaaacgc ccagcgagat ttgaaccggt
    17581 agctcaaaca ggcgagtaac cattaccggc accgggcgct ctggaataaa acttttacgc
    17641 agcgcctgga actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta
    17701 atgctggcaa gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag
    17761 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgt
    17821 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    17881 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    17941 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    18001 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    18061 tgccatccgg cggcggaata acgtacgtag agcaggaaat ggctgggtgc gtaatctttc
    18121 gcctgctgga tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt
    18181 aatgcccgcg ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    18241 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    18301 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    18361 tcccgccaca tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc
    18421 tgaccgagtg agtagccact cagatctttg tcgtaatgcc agatcatgtt gttcaggccg
    18481 attgtgacgc ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc
    18541 acgcgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    18601 acgccgtttg cttcaccttt atcgtagctc agacttagcc ccacaccgtc ggcacgtacg
    18661 ccaccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    18721 gaactggaga tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    18781 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtaccgata
    18841 tcccagctcc agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg
    18901 ctgcggttgc cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttgcca
    18961 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    19021 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    19081 taaccaccgg tgccgctcga accccagtaa tcgtgctcga gggtgacgtt aagatcctgc
    19141 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    19201 tcgttacggg tcagtcgggt gaatgtgtcg ttatcctgcg gacgcgtcgt agtcacaccg
    19261 gatgcgacca tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct
    19321 tcaaacttcg caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt
    19381 gcctgcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc
    19441 tgcgccagcg ccacgcgccg ctgtgtgttc agcgaggcgt tatcggtagc gggcagtttc
    19501 gccagctggc tacgtgccgc cgctgtgtca ccggcagcaa tatccacttc cgtcagcccc
    19561 agaatggcat cggcgttagt tggctccagc gtcaggacat tctgatatgc agcgcgggcg
    19621 gcggtgtaat cacgtcgctg ctgcgcccag tcagccagcg tgaggtcaat acgcgtggaa
    19681 ggtggttgct gacgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga
    19741 ttagcggttt ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    19801 ttccactgcg cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggtcatga
    19861 ccagagaggt acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    19921 agattgcgca ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa
    19981 agtcggtaag taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct
    20041 gccgcctgcg cccatttgcc ctggttttcc agtgcctctg cctgctgtgc cagacggtcg
    20101 ttttgcaggc tgcgttcgat atcatcaatg ctacgccgct gactggcaga gagcgaggcg
    20161 ataaacgctt cagctttttc tggcgattgc tggcggtaaa tatttgccag cccgcgcacg
    20221 gcgttagtgt tgccgctgtc catacgcaag gtctgctgat aataacgttc ggcggcggga
    20281 taatcttttc gcgccatcgc cacatcgccc agccccagca ctgcataact gtcggtgtta
    20341 tcgacattac gcgcctgctg gaacaggcgt tctgcccggt caggattatt ggctttcagc
    20401 gcagcatcgc cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat
    20461 ttgtcgttgt tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca
    20521 ttggcgcgat cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct
    20581 ttcgggttcg cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag
    20641 tccaccgccg ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc
    20701 tgttctgcca gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga
    20761 tattttttca gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac
    20821 cagattttag aggccccttc gcgcccggcg ttcgattttg ccatctgttc caggacggca
    20881 aaaccttcgt cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc
    20941 gtattgcccg gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga
    21001 gccggaattt tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca
    21061 ccgttgaaca gtttgttgta actcgccaca gcttcttctg catgaccggt cgtcgcctgc
    21121 aatcgtgcct gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc
    21181 gacgatttat acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc
    21241 gcgccatcaa tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg
    21301 ttattcggat caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg
    21361 gtcgcttcgc ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg
    21421 accatcggca tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc
    21481 attcctggcc ccagtcaggt aataactcac cttttgtcga gaagcggaaa cggtgttgat
    21541 cccagccttg tccaaacagg gtcagcacat agttgtaata ggcatcgctg ccgggaaagt
    21601 tatcggccac gcgctggcgc tgaacggcct gcgcatcgcg gttttgtaaa aagggcagca
    21661 tggcggcaga aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt
    21721 tttccggcgg ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca
    21781 tccgcgcttt ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag
    21841 cgtcgtagct gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat
    21901 agcgcaccca gtctggcgaa aaacctttcg gggcagtttc cagcaataaa cgttgattgg
    21961 tttcgcgcag cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg
    22021 gcaggtagct ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta
    22081 acaacatgga acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca
    22141 acgcgctgcc gatgtcagta taacgctgct ctttccacaa acgccccgcc tccagcaacg
    22201 accaggccat ccagatatca ccatcggagg ccgaattgct gtccagcact tcccacttac
    22261 tgttctcttt cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga
    22321 gattgttctg cgtccagtcg agaatattat cgaaagctgc acggtcgtta gccgccaggg
    22381 caaagaacat gccgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt
    22441 cgatgacgcg cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggccagg
    22501 tacaggctgc ctgaacacta aaggcagcca gcagcagcat cgtcacgatt ccactacgca
    22561 acacattcat cttcaattac tcgttatccg ggttaagacg acgacgacta ataattcgca
    22621 gcagacgcca cagtacccat gccagcaata tcacactgat agccgccagc accgccagca
    22681 gaatcggatg gtttgccagc gcataccaca cgcgctcgaa ccacggcaga tgacctacgt
    22741 aataaacgtc gccaacacgt aggctgttga taccggactc gcggatcacc gcgaccgaac
    22801 cgaacatggt ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc
    22861 gtgggctatc tgccaacagc gcaatcacgc tgcgctggtc gttatacggc gactggaagc
    22921 caatcaccgc cgccatcgca ccggaagagg tcagcgttga ccgggtttct gccgcgcgat
    22981 cgctctcgtc cggcacaatg ccggggaacg gggtctggcg catcggtgtt ttcacccagc
    23041 tttcggtcgc ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg tccgggatac
    23101 caccgatgat catgatgtcg gcatctttgc cctgaatggt gctgccatca tcggtcaccg
    23161 tcagattaat cgccgggaag cccgtctgtg cgccgataaa accaacagta ttcagcaacg
    23221 tttccatctg tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc gacagatccg
    23281 ccatccggct gaatgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga
    23341 agtggtaata cttcgagaag tcgatggtgg agtcgtcacc aatcaccaca tgattctgca
    23401 ccggctggaa ggtaatacag ttatccaccg aaccgcccgg catcgggttc atatactcaa
    23461 agtcgaagcg cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatctgttt
    23521 tgccatccag caaaccttgt aataccggaa tccgcagcag caggcggttc gcctcctgtt
    23581 tgctgctcag attgaaggat tgcaggaact ggttattcag gctgatatcc atccgcgaac
    23641 tgtctttcac cggcggcatg gtgtagcggt aattaatatc catatcaatg ccggtactgc
    23701 gcatcaggta gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaagac
    23761 cgctggattg taactgttct tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg
    23821 tacgtaccca gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca
    23881 ctaccacgct ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca
    23941 acaagtcttt gtcgtcacga ccaaacacca ccagcagttt gacgtaagga ttctgcggat
    24001 ggttaatcat ctcaatcaca ggggctttta ccgccggatg atcgcgcagg aagtccggcc
    24061 gtttgtcgtt ggttgcaaag acaatggcat tacgatccgg cagttggtta tagagcaccg
    24121 ggaagttctg cccacgccag ccagaacgcg aaccaaacca cgaggcgaca atggcagagg
    24181 cttgttgcag cccaacatcc ggcgcacccg caaagaccat cggcaaggtg ttagtacggt
    24241 tatcgcgcgg gtcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg
    24301 tctgataggt cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg
    24361 ccgggttttc gcacacgtcc tgataatggc cgacaaactc cagccgcaca cggttgaagt
    24421 cggtaataaa cagtgggtta atgggcattt gcgccagcgt ttttttaccc aactgttctt
    24481 tggtcactgg cagcacgccc atcagctcat cattgagata aacctttaac tgcgactgga
    24541 caggcagtaa cgatggcgat ggcgtgtatt cgaggttgag catcgccttc gtcaccactt
    24601 catcgctgcg catgccaaac tcaatgctgc cgttcgggtt aatgccacgt agcaccatgc
    24661 tgcccggcgg cggcgcaatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct
    24721 gcgccacgac tggcgcatca gcgccctgca cgcccggcat cacttgcccc acctgcggat
    24781 tttgttccgt ctgggcggct acagctggct cagcattgat cagtggttac gttgctggcg
    24841 tcgcctgcgt catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga
    24901 atagttttct tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt
    24961 gccgtttcgc tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa
    25021 gtcagcacac ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag
    25081 ccacggaagc cgagcttcag aatatccagc agactttcca gcggcttatc ttccgggtag
    25141 ctgtcctgcc agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata
    25201 tgttgctggg tggtgagcgg cattaatttc agcccaactt cattacccat cacgcgcgcc
    25261 acctgggtcg ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc
    25321 ccttccagaa tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc
    25381 tgaacggtac acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc
    25441 tccacgcggt gcgatcggcg tacctgtttg ctttctaccg atactgcaac tgcgccgcca
    25501 agaacaatca ggttgtagaa tacccacacc atactgacga ccacggtgag catctcggtt
    25561 ggcgggccat agaagtagcg ccagatgcct accgcaacgc ccaccaggtt gagcaggaca
    25621 aggaagatgt agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct
    25681 ttggcggtga cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt
    25741 gcgatatacc acgccagcac cgtttcgtag atttcactcc agaaagagtg gcgatatttg
    25801 ccctggatct tggagttggt caggctggca tggatcatat gcggcagcac gaacagggcg
    25861 atcatcaacg ctggcgcata gatgatgtag gcatgaagca gcaggaacgc cagcggcgca
    25921 gtcaggaaga tcagtcgtgg aatgcccgac aagaagtgga acatggcgtt gacgtagcac
    25981 agccgctggg cgaatttcag ccctttaccg gtaagcgggt tatcgagacg gaagatttgc
    26041 accatcccgc gtgcccagcg aatacgctga ccgatatgcg ccgacagact ttcggtcgcc
    26101 agccccgccg cctgcggaat acgcatatat gcagaggtgt agccacgacg gtgcaggcgc
    26161 agagacgtgt gcgcatcttc ggttaccgtt tcgacagcaa taccgccaat ttcatccaat
    26221 ggtttgcgac gaattactgc acaggaaccg cagaagaaag tggcgtccca catatcgttg
    26281 ccatcctgca ccagaccata gaacagcgtg ccttcgttcg gcgttttacg aaaacgcccc
    26341 aggttgcgtt caaacgggtc cggcgagaag aagtggtgcg gcgtctgcat catcgccagc
    26401 tgtttctctt tcaggaacca gcccatggtc atttgcagga acgatcgcgt tggtacgtgg
    26461 tcgcagtcga aaatcgacac gaactcgcct ttggcatatt tcagcgcatt gttgatgttg
    26521 cccgctttcg catgttcatg agtggtacgg gcgatatatt tcacccccac gttttgcgca
    26581 aactggcgaa actcttccct accgccgtca tcgaggatcc agatgttcag cttgtctttc
    26641 ggccagtcga tacccagcga ggcgtaaata gtatttttca ccacgttgag atcttcgttg
    26701 taagtcggga caaagatatc caccgacggc cacagcgaca tatctttcgg caatggcacc
    26761 ggctgacgat tcagcggcca tactacctgg aagtagccga gcaccagcac aatccacgcg
    26821 tacgtttcag cgaagagcag aataagcccg cacaccaggc tgaccggatc gtcccagttc
    26881 agcgtagagg tgtaacgcca ccagatataa cggcaagaaa cggtcagcga cagcacaatc
    26941 aacattagcg ccgagaagcg ccccggcatc cgccgtacga tcagcgctac cccccacagc
    27001 agcatcagga agataaactg cgccagcggg ttaaacggct gagtaacgca gattaacgcc
    27061 agaatcagcg agaaggtgac gatgataccg aggatcaacc gccgcgcccc ggcactcaaa
    27121 tgaccgagct cttttttctc atccagatgc tgtgttttat ggctaacgcg ctcaggcagc
    27181 tcgttcatcc attgatggta acgtccacga atattttgca gacctgaaaa tgcccgcctg
    27241 cgcggtttcg gcgtttcttt gcgcgatgca ccgatcaata accagcatgt ttgaatgaga
    27301 taacggaccg ggtccagcgg acgcggacgc gaggcgttga tatgcggata caggttttta
    27361 tgttctgcgc gaatacgctg ccagcgcggg tgctccagcg gaataaaaat ccaggccagg
    27421 atcatccaga aacagccgag cgtcgcgctg aaagccgacg caccgtggcg acgataatcg
    27481 cgataacgcc cgataagccg cgcgttgacc ggcgggataa gcaaccaccg ggtcaggata
    27541 ctcatgatgc actcccgact ggcgttttca gcccggaata gttcaacagg caccagttcg
    27601 ccagcgtcag tatctcttct gccgccagcg catcactgcg gtattcaccg actggttgct
    27661 tagccgccag gcattcagcc atcgcttcat cacgatgaat gagcatcggc agcaatcggc
    27721 gctggctttg caaccaaagc tggtaaatat cgtcctgaac ctgactgcca atacggaagt
    27781 tattaatcaa aatatgtgcg ccatccggca gcgcttgctg atgcaggcgg atatggcagt
    27841 tggcatccac attgacgata gccagcgagt gatcgcacaa actcagcagc tggtgggtta
    27901 tctgcgaggc atcacgcggt aagtcgatta aaatccactg gtaacgcccg ctggctttta
    27961 gttgctgtaa gccggagcaa atatcgctca gccgggtttg ccagtgctgt ggattttctt
    28021 gttcttcaat ggataactga ccaaaaggca gcaaatcgag ctgcgaggtg tagcgcaacc
    28081 cagcgtcacg ccagtcctgg ccatccagca tcgctctggc ccagccctga cggtgggtaa
    28141 aatcaacatt aaatgacagg cgcaacaagt tgtccgggca ggcatcgacc accagaacat
    28201 tttctcccaa catttgtaat gaccaggcta atgcggcggt gatggttgtt gtccccacgc
    28261 ctccccgcac cccctgcaat cccagtacgg ccattcacca atcccctact tttgttgcgc
    28321 aaactctgcc agcaacggcc agcgttttaa tgccgcggcc aactgttcgc gttgggaaat
    28381 atcggcataa tcaatatcag gcagtgaaaa tgcctgcttt aacgccacaa tatcattctg
    28441 gaagatatag cctatcgcgg gatcaggcag agtatctggt tcattgttat tcattttgct
    28501 tgatccctat gaaataaccg taccgttcat gagacaattc taaccgtgag catttcgccg
    28561 gtttattgtt tacggcgaga cgggaatttg ctcacttttt gactttccgt tacgcaacag
    28621 aaaaagttaa aaacgaaaag actaaaatca cacgtcttgt gaccttctaa attgattcac
    28681 atgctaaatc tgataagttt taatttcaat ggtaggttta tttcttagct ttcgctagta
    28741 aactgataat cagacaaaat ggtgacatga gggacattgt ggaccctgta ttctctatcg
    28801 gtatctcatc attatgggat gagctgcgac atatgccagc aggcggcgtc tggtggttta
    28861 acgtcgatcg ccatgaagat gctatcagtc tggcgaatca aacaattgca tcccaggctg
    28921 aaaccgcaca cgtcgcggtc attagcatgg acagcgatcc ggcgaaaatc tttcaattag
    28981 atgattctca agggccggaa aaaataaaat tattttcaat gctaaatcat gaaaaaggtc
    29041 tatactattt gacccgtgat ttgcagtgtt ctattgatcc ccataattac ctttttattc
    29101 ttgtttgcgc aaataacgca tggcaaaaca ttcctgccga gcggcttcgc tcatggttgg
    29161 ataaaatgaa taaatggagc aggttaaacc attgttcgct tttggtaatt aatcccggaa
    29221 ataataacga taaacaattt tcattgttgc ttgaggaata ccgttcactt tttggtcttg
    29281 ccagtttgcg ttttcagggt gaccaacatt tgctggatat tgccttctgg tgcaacgaaa
    29341 aaggggtcag cgcccgtcag cagcttagcg ttcagcaaca aaatggtatc tggacattag
    29401 ttcaaagcga agaggcggag atccaaccac gcagcgacga aaaacgcatt ctgagtaatg
    29461 ttgctgtact ggaaggtgcg ccgccactat cggaacactg gcaactgttc aacaataacg
    29521 aagtcctgtt caatgaagcc cgtaccgctc aggcggcgac ggtggtcttt tctttacagc
    29581 aaaatgcgca aatcgagcca ctggcccgca gcattcatac cctgcgtcgc cagcgcggta
    29641 gtgcgatgaa aatcctcgtg cgggaaaata ccgctagcct gcgcgccacc gatgaacgtt
    29701 tgttattggc ctgcggtgca aatatggtta ttccgtggaa tgcgccactc tcccgttgtc
    29761 tgacgatgat cgaaagcgtg caagggcaga agtttagtcg ctatgtgccg gaagatatca
    29821 ctaccttgct gtcaatgacc cagccgctca aactgcgtgg tttccagaag tgggatgtgt
    29881 tctgtaatgc cgtcaacaac atgatgaata accctctatt acctgcccac ggtaaaggcg
    29941 ttctggttgc cctacgtccg gtaccgggta tccgcgttga acaagccctg acgctgtgtc
    30001 gccctaaccg taccggcgat atcatgacca ttggcggtaa tcggctggtg ctgtttctct
    30061 cattctgtcg gattaacgat ctggataccg cgttgaatca tattttccca ttgcctactg
    30121 gcgacatttt ctcaaaccgt atggtctggt ttgaagatga tcaaatcagt gccgagctaa
    30181 tgcagatgcg cttgcttgcc ccagaacaat ggggcatgcc gctgccttta acgcaaagtt
    30241 ctaaaccggt catcaatgcc gagcacgatg gtcgccactg gcgacgaata ccagaaccca
    30301 tgcgactgtt agatgatgct gtggagcgct catcatgatg accatcagcg atatcattga
    30361 aattattgtc gtttgcgcac tgatattttt cccgctgggc tatctggcgc ggcactcttt
    30421 gcgacgcatt cgcgacacct tacgtttgtt ctttgctaaa cctcgttatg ttaaaccggc
    30481 cgggacgtta cgccgcacgg aaaaagccag ggcaaccaaa aaatgactca atttacgcaa
    30541 aataccgcca tgccttcttc cctctggcaa tactggcgcg gcctttccgg ctggaacttc
    30601 tattttctgg ttaagttcgg cctgttgtgg gcgggatatc ttaacttcca tccgctcctc
    30661 aatttggtgt ttgccgcgtt tctgctgatg cccattccgc gctacagcct gcatcgcttg
    30721 cgccactgga ttgccctgcc gatcggcttt gctttgttct ggcatgacac ctggttgcct
    30781 ggcccggaaa gcataatgag ccagggttcg caggtggcgg ggttcagtac cgattattta
    30841 atcgaccttg tcacacgctt tattaactgg cagatgattg gggccatttt tgttttatta
    30901 gtggcctggt tattcctgtc acaatggatt cgcattaccg tttttgtggt tgccatactg
    30961 ctatggctga acgtacttac cctggcggga ccaagtttct ccttgtggcc agccggacaa
    31021 ccgacgacca ctgtaacaac gacgggtggt aacgcagcgg caaccgttgc ggcgacgggt
    31081 ggcgcaccgg tagtgagtga tatgcccgca caaactgcac cgccaacaac ggcgaacctt
    31141 aacgcctggc tgaataattt ctataacgcg gaggcaaaac gtaaatcgac cttcccgtct
    31201 tcgctgcccg ctgatgctca gccatttgaa ctactggtga ttaacatctg ttcgctttcc
    31261 tggtcggata tagaagccgc cgggttgatg tcgcatccac tgtggtcaca tttcgatatt
    31321 gagttcaaga actttaactc cgccacctcc tacagtggcc cggcggcgat ccgtttactg
    31381 cgcgccagct gcgggcagac ttcgcacact aatctgtatc aaccggcaaa taacgactgc
    31441 tatctgtttg ataacctttc gaaactgggc tttacccagc acctgatgat ggggcataac
    31501 ggccagttcg gcggtttttt gaaagaagtt cgcgaaaatg gcggcatgca gactgaattg
    31561 atagatcaaa caaatctgcc ggttattttg ctgggctttg atggttcgcc ggtttatgac
    31621 gataccgccg tgcttaaccg ctggctggac gttaccgaaa aagataaaaa tagccgtagt
    31681 gccacgttct acaacacgct tccactgcat gacggcaacc attatccggg ggtcagcaaa
    31741 acagcggatt acaaagcgcg ggcgcagaaa ttctttgatg aactggacgc cttctttact
    31801 gaactggaga aatcgggtcg taaagtgatg gtggtcgtgg tgccggaaca cggcggcgcg
    31861 ctgaagggcg acagaatgca ggtatctggc ctacgtgata tccctagccc gtctatcaca
    31921 gacgtccccg ttggggtgaa attcttcggc atgaaggcac cacatcaggg ggcaccgatt
    31981 gtcattgacc aaccgagcag cttcctggct atctccgatc tggtggttcg cgttcttgat
    32041 ggcaagattt tcaccgaaga caatgttgac tggaaaaaac tcaccagtgg gttgccacaa
    32101 acagcaccgg tctccgagaa ctcaaatgca gtagttattc aataccagga taaaccgtac
    32161 gttcgcctga acggcggcga ctgggtgcct tacccgcagt aaacacgaaa aaggccgcag
    32221 agtttaacct ctgcggcctg atccgggcgc aaattgccat tacggcagcc tgacgcccgc
    32281 atggcacgtt acttccgctt gttcagccag ttcacgatca tactggcaag aataccagca
    32341 atgaccggag ccgctaaatc atgccagaag gccatgccca gttctgcgag cgtcatatag
    32401 ccgcctgtat tgtaatgaca acgtttcgcg gctattcttg tattgtttgg atgcaagaat
    32461 ggcccccgtg gtgttgtcag gtgcatacct gcaacgtgcg ggggttttct ctctccagca
    32521 accaatgcca ccagggatta agcccccgca acattgcgcc tcaccggaat ccttccggct
    32581 tgacgctgat tctacgactg tttttctgtt ccggaatccg tctcatcttc actctgtgct
    32641 gcgcattccg cagaatatta tccgcttgca accgttgcat ttttcttacg aaaaaggccg
    32701 cagagtttca ctcctgcggc ctggtccggg cgcaaattgc cattacggca gcctgacgcc
    32761 cacatgacac gctacttccg cttgttcagc cagttcacga tcatactggc aagaatgcca
    32821 gcaatgaccg gagccgctaa atcatgccag aaggccatgc ccagctctgc gagcgtcata
    32881 tagccgcctg tgttgtaatg acaacgtttc gcggctattc ttgagtggtc taggttcaag
    32941 attagccccc gttatgttgt caggtatata cctgcaacgt gcgggggttt tctctctcca
    33001 gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg aacccttccg
    33061 gcttgccgct gattttacga ttgcttttta ctgtcagcac ccggcaatct tcctcattgc
    33121 gcgatgcctt ccagcttaat ttctttattt gcaatagttg cagttcatat ccgggctgac
    33181 tccagactgg tagtgaaatg cgcaattttg tagaattgcg cctcctcgcg acctccccag
    33241 tgaatctgtt gcctcatttt ttacatactg cgtattcgac ttctccacct gttgcgcaag
    33301 agaaactggg tttattcatt tttgcgaggc cgacttcttt ctggacagga ctttatgcag
    33361 cacaacacac tatcgaaaca caatcagaaa ttgccgttta cacgctacga cttcggctgg
    33421 gttttattat gcataggcat ggcgattggt gccggaaccg tgctgatgcc agtacaaatt
    33481 ggcttgaagg gaatttgggt atttattacc gcagcgatca ttgcttatcc tgccacctgg
    33541 gtagtgcagg acatttattt aaaaaccctt tctgaaagcg attcctgtaa tgactacacc
    33601 gatattatca gtcattacct ggggaagaac tggggaattt tcctcggggt tatctacttt
    33661 ttgatgatta tccacgggat ttttatctac tctctctccg tggttttcga cagcgcctcg
    33721 tacctgaaaa ccttcggttt aaccgatgcc gatctttcac aatctctact ttataaagtc
    33781 gctattttcg ccgtactggt ggcgattgcg tctggtggtg aacgattact gtttaagatt
    33841 tccgggccaa tggtggtggt caaagtaggg attattgtcg tgttcggttt tgcgatgatc
    33901 ccgcactgga atttcgccaa tataaccgcc ttcccgcaag cctccgtctt tttccgcgat
    33961 gtcctgctta caattccatt ttgcttcttt tctgcggtat ttattcaggt acttaaccca
    34021 atgaatattg cctatcgtaa acgggaagca gataaagtac tggcaacccg gctcgcgctg
    34081 cgtacccacc gaattagtta tatcacgctc atcgcggtga tcctgttttt tgccttttcg
    34141 tttaccttct caattagcca cgaagaagcc gtttctgcct ttgaacaaaa tatctcggca
    34201 ctggcgctgg ccgcgcaggt gatccctgga catatcattc atatcacctc tacggtgctg
    34261 aatatctttg ccgtactgac cgcattcttt ggcatttatc tcggtttcca cgaggctatt
    34321 aaaggcatta ttctcaatct gttaagccga attattgata ccaagaaaat taactcacgc
    34381 atgctgactc tggcgatctg cgcttttatc gtcattacat tgacgatttg ggtttcgttt
    34441 cgtgtatcgg tgctggtgtt ctttcagttg ggaagcccgt tatatggcat tgtgtcgtgc
    34501 ctcattccgt ttttcctgat ctataaagtc gcacaactgg aaaaacttcg cggatttaaa
    34561 gcctggctaa ttctgcttta cggcattttg ctatgcttgt cgccactgtt gaagctgatt
    34621 gagtaaaccg gagcgcatgg ccccggtttt gtgagttaac gctgcggatt ttcatcctga
    34681 tcaacagcaa aacaagcgac cagttgaccg ccgtagtctt ttagctgcgg ctgcaactgg
    34741 gtgcaggggc cgaagcgccg acgacagcgg gcgttgaagg cgcaacccgg cggaggattc
    34801 agcgggcttg gcagttcacc ggtgagtttg atgcgttcac gacgatcgtc cgggttcagg
    34861 cgtggcgtcg cggagagcag cgcctgggtg tacggatggc gcgggttatt gaagatttgg
    34921 tccttcgttc ctttctccac gcagcggccc aggtacatca ccatcacttc atcagcaatg
    34981 tgctccacca ccgacaggtc gtgggagata aagacataag aaagccccaa ctcctgctgc
    35041 aaatccatca tcagattcag cacctgcgca cgcactgaga cgtccagcgc agaaaccggt
    35101 tcatcggcaa tcaccacgtc cgggtcgagc atcagaccac gggcaatagc gatacgctga
    35161 cgttgtccgc cggagaacat atgcggatag cggtcatagt gctcggtttt caggccgact
    35221 ttcgccatca tcgacagggc tttttcccga cgctgttctt tgctgaagct ggtgttaata
    35281 agcagcggct cttcgagaat ttgcccaact tttttacgcg gattcagcga accgtaaggg
    35341 ttctggaaga caatctggat tttctgccga cgcagcttct gcgcctgcgg atcgtgcttg
    35401 agcagatcct gcccctggta atacagctca ccaccggtgg gcgtttcgat catcgtcagc
    35461 aaccgaccga gggtcgattt accgcagcca gattcgccca ctactgccag cgttttgcca
    35521 cgttcaaggt taaacgaaac gccgtccagc gccttcacca gacgttccgg cgcgaacatg
    35581 cctttcttca ccggataatg ttttttcagg tcgatagcct gcaacagcgg ttgttgcgag
    35641 gtggcctctt gcgtactcat agtgtcggcc tcccggcatc atcaagtggg taatggcatt
    35701 tggactgacg cccatcagcg agcatattca gcgccggttc ttcagcgcga catctgtcag
    35761 tggcataggg gcagcgcggg ttaagcaggc agccgttcgg gcggtcgtac ttaccgggaa
    35821 cgacacctgg caacgacgcc agacgttctt tgtcctgagc aaattctggc agcgcacgca
    35881 gcaatgcctg agtatacggg tgacgcggcg catggaagat ggcgtgcgca tcaccggttt
    35941 ccaccacctg gcctgcatac atcacgatga ttttatgtgc cgcttccgcc accagcgcca
    36001 ggtcatgggt aattaacacc agcgccatgt tctctttctg ctgtagctcc agcagtagtt
    36061 cgatgatttg cgcctgaatg gtcacgtcca gcgcggtggt cggttcatcg gcaatcagca
    36121 gttttggccg acaggcaatc gccatggcga tcatcacgcg ctggctcatg ccgccggaaa
    36181 gctgatgcgg gtaaatatcc agacgcgatg ccggatcggg aataccgacc tgattcagca
    36241 ggtcaatcgc ccgctgacgg cgagtgctct tattaccgcc ctgatgcacc ttaatcgctt
    36301 ccatgatctg gaaacccacg gtgtagcacg ggttaaggct ggtcatcggg tcctggaaga
    36361 tcatcgccac ttcggcaccc accaggttgc ggcgctcttt ttctgatata cgctgcaaat
    36421 cctgaccgtt aaacaccagc ttttccgcca ttacgcggcc cggataatca atcagcccca
    36481 taatcgccag tgaactgacc gacttaccgg aaccagactc acccacaatc ccgaccactt
    36541 cgccctgttt tacgctgtag ctgatgcggt ctacggcgcg gaacggtgcg ctttcgtcgc
    36601 cgaaatgcac cgataattta tctacattta ataacgccat ctcgaacctc ttactgcttc
    36661 agtttgggat cgagcgcgtc acgcagaccg tcacccatca ggttaaatgc cagcaccgtc
    36721 agcaggatcg ccagacccgg gaaggtcacg acccaccagg cgctttgcgc gaactgcaac
    36781 acgtcggaga gcatggtgcc ccactctggt gttggcggct gcgctcccat gccgaggaag
    36841 ccaagggcgg ccatatcgag aatggcgtta gagaaaccga gcgacgcctg aacaatcagc
    36901 ggcgcaaggc agttcgggaa gatgttaata aacatctgac gcatcgcccc ggcacccgcc
    36961 acgcgagacg cggtgacgta atcgcggtta acttccacca gcacggcggc gcgggttaag
    37021 cgcacatagt gcggcaaggc aacgaaggtc agtgccagcg cggcgttacc aatcgacggg
    37081 ccgaagattg ccaccagcac cagcgccagc agcagacttg gcagcgccag catgatatcg
    37141 accacgcgca taatgatgtt atcgaccagg ccgccaaagt aaccggcgat caggccgaga
    37201 ataacgccca taatcagcga taacacaact accagacagc caaccagcag cgacaggcgc
    37261 gcaccgtaca tcaggcgcga cagcacatca cggcctacgt catcggtgcc cagcaagtgc
    37321 gccatgctgc cgccttcctg ccaggctggc ggggcgagca gtgcatcgcg gaactgttcc
    37381 gccgggttat agggtgcaat ccagttggca aagatcgcga tgaacagcac gatgacgacg
    37441 taaaccagcc cgacgaccgc gcctttgttg cgtttaaaat agtgccagaa ctcctgtaac
    37501 ggggtcatcg gcaccggtgc gctaatcact ttattttcag taacctgtga catgatgtcc
    37561 ccttacttct tatgacgaat acgcgggttc accacgccgt acagcagatc gaccagcaag
    37621 ttgacgagga taatcatcgt cgccaccagc aatacgccgc cctgcactac cggatagtcg
    37681 cggcgttgca gcgcgtcaat caaccagcgc cccagaccgg gccacgagaa gatggtttcg
    37741 gtcagaatcg cccccgccag caatgttccc acctgcaggc cgataacggt cactaccggc
    37801 agcatcgcgt tacgcaacgc atggacgata atcacccgca tgcgggttag ccctttggcg
    37861 cgcgcggtgc ggatgtaatc ctcgcccagc acttccagca tcgaggagcg tgtcatacgc
    37921 acaatgaccg ccagcggaat agtacccagc acaatggcag gcaagatcat atgggcgacg
    37981 gcatcgataa agttgccgtc ttcaccccag atggcggtgt cgattagcat aaaaccggtt
    38041 aacggattgg agtcatcgag gaacaccata tcgctcacgc gaccggagac gggcgtcagg
    38101 ttccagtgca ccgaaaccag catgatcagc atcatgcccc accagaagat cggcatcgag
    38161 tagccggtca gcgccaggcc aaccgctgtg tgatcgaaaa tggaaccgcg tttaaccgca
    38221 gccagcacgc cgaccggaat accgaccgca gtcgcaaaaa tcatcgcgca gacgccaagt
    38281 tccagcgtgg cctggaggcg cggcacgaac tcttcccaaa ccgggatgcg gcttttcatt
    38341 gaaatgccta gatcgccatg cataacgccc caaatgtaat ggagatactg ctgccacatc
    38401 ggtttatcta agccgagttc agccagcagc tgcgcgtgac gctctgggga gatcccacgt
    38461 tcgcccgcca tgatcatcac cggatcgccc gggatcatgt ggacaaaggc aaatgtgaga
    38521 agggtaatac cgataaacgt ggggatgacg agtcccaaac gtcggagaat aaactgcaac
    38581 ataacccgga ttctctatag tgacccgcag cctggaactg cgtgtctgta ttgctcacaa
    38641 atcaattccc gcccaggtcg ggctgggaat gaagtattgc tgccggatgg cgctgcacgc
    38701 agaggcacag cgccaccggg tatggctttt aattattcga tagagacgtt ttcgaagtga
    38761 tgtttgccta atggatcaac cacatagcct ttaacttctt tgcgtaccgg ttcaaacacg
    38821 gtggagtgag cgatgatcag tgccggagcc tgatcgtgca tcaccacctg cgcttgcttg
    38881 tacagttcaa cgcgtttgtt atggtcgtcg gtagcacgcg ccggttgaat cagatcttca
    38941 aacggtttgt agcaccattt tgagtagttg gagccttgtt cagaggcggc gcagctgaac
    39001 agggtggcga agaagttatc cggatccccg ttatcgccag tccagcccat cattaccgtc
    39061 tggtgctcgc catctttcgc acgcttgagg tactcacccc attcgtaggt gacaattttg
    39121 gcctgcacgc cgactttcgc ccagtcagcc tgaatcatct ccgccatgcg gcgagcgttc
    39181 gggttatatg ggcgctgtac cggcatcgcc cacaggtcga tggagaaacc tttttccaga
    39241 cccgcttctt tcagcaaggc tttcgctttt tcaggatcgt aggtgtaatc ctgaacgtcg
    39301 tcgttatagc cccacatggt tggcgggatc aggtttttcg ctgatacacc cgcgccctga
    39361 taaaccgctt tgatgagcgc gtctttgttc accgcgtagg tcagtgcctg gcgaactttc
    39421 acgtcatcca gcggtttttt ctgcacgtta taggagaggt agccgacgtt cagccccggc
    39481 atttccatca ggttgatgga tttatcctgc ttcatgcgag cgatgtctgc cgggttcggg
    39541 tacggcatca cctggcattc attcttctgc aatttcgcat aacgcacgga agcgtcagga
    39601 gtaatagaga agaccagcgt atcgatctgc ggtttggtgc cccagtagcc atcaaacgct
    39661 ttgtagcgga tacgggagtc tttttggtac tgctgtaact ggaacggacc ggtgccgatt
    39721 gggttgaggt ccagtttttc cggtgtaccg gctttcatca tcgcgtcagc atattctttt
    39781 gacagaatag aggcgaagtc cattgccagg tcagcgagga acggcgcttc cgggcgagtc
    39841 agcacaaact gaacggtgtt gtcgtccacc tttttcactt cgctgatcag ctccggcaag
    39901 cccatgcctt cgaagtattc gtagctgccg ccagaaactt tatggtacgg gttttgcgcg
    39961 tttttctgac gatcaaacga gaacaccacg tcatcggcgt tcagttcacg cgtcggtttg
    40021 aattctttat tgtcgtgcca cttcacacct ttacgcagat ggaaggtata ggttttaccg
    40081 tcttcgctga cttcccactt ttcagcgagg cccgggatca cttcagtggt gccgatttta
    40141 aattcaacca ggcggttata aagcggtacg gaagaggcgt cataggtggt gccggaggta
    40201 aacagctgcg ggttaaaccc ttccggagat ccttctgagc aataaaccag agttttagcc
    40261 tgaacacttg ctgcgacggt catagccacc aggctgagac caagcttcag catccctgac
    40321 tttttcaagg aaatacgcat tattctgctc caattgtgat gtttgttgtt ttaacccttt
    40381 gcagtgggtt gtcgctgcct gacctttttt gttttttgcc cggtcgggtc aacgttatga
    40441 ggtggggatg ccgtactaat taacatcagt gtaacagtgc ggatcctcca taaaatgccc
    40501 ctcgtgacct acaatctgtc aacagaatgt gaaaacgtca atacagccaa ccgggattta
    40561 caccaacggt gagaatccac aaacaaagat taaaaaaact tcaaacagct atttgcagca
    40621 gagaaatttg tgctactcca acatgaccag aacaatcagc ttaatattta gcaacatttg
    40681 gtgattaagt tttatgcatt ta
//
LOCUS       DAZUFB010000034        40427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZUFB010000034 DAZUFB010000000
VERSION     DAZUFB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.034"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..495
                     /locus_tag="ACHY3M_002023"
     CDS             <1..495
                     /locus_tag="ACHY3M_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFS4316943.1"
                     /translation="LYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINEN
                     KEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNK
                     VTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIR
                     QGSN"
     gene            497..818
                     /locus_tag="ACHY3M_002024"
                     /pseudo
     CDS             497..818
                     /locus_tag="ACHY3M_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(905..1580)
                     /locus_tag="ACHY3M_002025"
                     /pseudo
     CDS             complement(905..1580)
                     /locus_tag="ACHY3M_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1725..2333)
                     /gene="yiiX"
                     /locus_tag="ACHY3M_002026"
     CDS             complement(1725..2333)
                     /gene="yiiX"
                     /locus_tag="ACHY3M_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFS4316944.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2518..2835)
                     /gene="metJ"
                     /locus_tag="ACHY3M_002027"
     CDS             complement(2518..2835)
                     /gene="metJ"
                     /locus_tag="ACHY3M_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFS4316945.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3112..4272
                     /gene="metB"
                     /locus_tag="ACHY3M_002028"
     CDS             3112..4272
                     /gene="metB"
                     /locus_tag="ACHY3M_002028"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4316946.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HQAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4275..6707
                     /gene="metL"
                     /locus_tag="ACHY3M_002029"
     CDS             4275..6707
                     /gene="metL"
                     /locus_tag="ACHY3M_002029"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFS4316947.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6846..6980)
                     /locus_tag="ACHY3M_002030"
     CDS             complement(6846..6980)
                     /locus_tag="ACHY3M_002030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316948.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            7056..7946
                     /gene="metF"
                     /locus_tag="ACHY3M_002031"
     CDS             7056..7946
                     /gene="metF"
                     /locus_tag="ACHY3M_002031"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFS4316949.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8275..10455
                     /gene="katG"
                     /locus_tag="ACHY3M_002032"
     CDS             8275..10455
                     /gene="katG"
                     /locus_tag="ACHY3M_002032"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFS4316950.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10549..11454
                     /gene="yijE"
                     /locus_tag="ACHY3M_002033"
     CDS             10549..11454
                     /gene="yijE"
                     /locus_tag="ACHY3M_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFS4316951.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(11481..12098)
                     /gene="yijF"
                     /locus_tag="ACHY3M_002034"
     CDS             complement(11481..12098)
                     /gene="yijF"
                     /locus_tag="ACHY3M_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFS4316952.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12373..13476)
                     /gene="gldA"
                     /locus_tag="ACHY3M_002035"
     CDS             complement(12373..13476)
                     /gene="gldA"
                     /locus_tag="ACHY3M_002035"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFS4316953.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13487..14149)
                     /gene="fsa"
                     /locus_tag="ACHY3M_002036"
     CDS             complement(13487..14149)
                     /gene="fsa"
                     /locus_tag="ACHY3M_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4316954.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14161..16662)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_002037"
     CDS             complement(14161..16662)
                     /gene="ptsP"
                     /locus_tag="ACHY3M_002037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4316955.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCRLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPMLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQAHTADGIRIEIAANIAHSVE
                     AQAAFGNSAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16971..18050
                     /gene="frwC"
                     /locus_tag="ACHY3M_002038"
     CDS             16971..18050
                     /gene="frwC"
                     /locus_tag="ACHY3M_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFS4316956.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     TAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18065..18385
                     /gene="frwB"
                     /locus_tag="ACHY3M_002039"
     CDS             18065..18385
                     /gene="frwB"
                     /locus_tag="ACHY3M_002039"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS4316957.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            18436..20733
                     /gene="pflD"
                     /locus_tag="ACHY3M_002040"
     CDS             18436..20733
                     /gene="pflD"
                     /locus_tag="ACHY3M_002040"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4316958.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            20699..21577
                     /gene="pflC"
                     /locus_tag="ACHY3M_002041"
     CDS             20699..21577
                     /gene="pflC"
                     /locus_tag="ACHY3M_002041"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFS4316959.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21579..21920
                     /gene="frwD"
                     /locus_tag="ACHY3M_002042"
     CDS             21579..21920
                     /gene="frwD"
                     /locus_tag="ACHY3M_002042"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS4316960.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21907..22758)
                     /gene="yijO"
                     /locus_tag="ACHY3M_002043"
     CDS             complement(21907..22758)
                     /gene="yijO"
                     /locus_tag="ACHY3M_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4316961.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22907..24640)
                     /gene="eptC"
                     /locus_tag="ACHY3M_002044"
     CDS             complement(22907..24640)
                     /gene="eptC"
                     /locus_tag="ACHY3M_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFS4316962.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24823..27474)
                     /gene="ppc"
                     /locus_tag="ACHY3M_002045"
     CDS             complement(24823..27474)
                     /gene="ppc"
                     /locus_tag="ACHY3M_002045"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFS4316963.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27826..28977)
                     /gene="argE"
                     /locus_tag="ACHY3M_002046"
     CDS             complement(27826..28977)
                     /gene="argE"
                     /locus_tag="ACHY3M_002046"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFS4316964.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            29131..30135
                     /gene="argC"
                     /locus_tag="ACHY3M_002047"
     CDS             29131..30135
                     /gene="argC"
                     /locus_tag="ACHY3M_002047"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFS4316965.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30143..30919
                     /gene="argB"
                     /locus_tag="ACHY3M_002048"
     CDS             30143..30919
                     /gene="argB"
                     /locus_tag="ACHY3M_002048"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFS4316966.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30980..32353
                     /gene="argH"
                     /locus_tag="ACHY3M_002049"
     CDS             30980..32353
                     /gene="argH"
                     /locus_tag="ACHY3M_002049"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFS4316967.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32620..33537
                     /gene="oxyR"
                     /locus_tag="ACHY3M_002050"
     CDS             32620..33537
                     /gene="oxyR"
                     /locus_tag="ACHY3M_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFS4316968.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(33520..34920)
                     /gene="sthA"
                     /locus_tag="ACHY3M_002051"
     CDS             complement(33520..34920)
                     /gene="sthA"
                     /locus_tag="ACHY3M_002051"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFS4316969.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35251..35901
                     /gene="fabR"
                     /locus_tag="ACHY3M_002052"
     CDS             35251..35901
                     /gene="fabR"
                     /locus_tag="ACHY3M_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFS4316970.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35901..36260
                     /gene="yijD"
                     /locus_tag="ACHY3M_002053"
     CDS             35901..36260
                     /gene="yijD"
                     /locus_tag="ACHY3M_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFS4316971.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYSLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36300..37400)
                     /gene="trmA"
                     /locus_tag="ACHY3M_002054"
     CDS             complement(36300..37400)
                     /gene="trmA"
                     /locus_tag="ACHY3M_002054"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFS4316972.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37514..37704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37769..39613
                     /gene="btuB"
                     /locus_tag="ACHY3M_002055"
     CDS             37769..39613
                     /gene="btuB"
                     /locus_tag="ACHY3M_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFS4316973.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGASL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39558..40415
                     /gene="murI"
                     /locus_tag="ACHY3M_002056"
     CDS             39558..40415
                     /gene="murI"
                     /locus_tag="ACHY3M_002056"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4316974.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9313 a        10060 c        11126 g         9928 t
ORIGIN      
        1 ctgtatacat atccgctgag cccggtgaat agcatggatc cattaggatt atatgaattt
       61 aaatcaaaaa atatagatga tattggaata tttgcattgg caatgtgtaa tggagaatca
      121 attaacgaga ataaagaata tggtggacta atatgtaaga agcaaggtga atatttcccc
      181 atgaatccga taagttcaaa tgataatgat agtgtagact tgcgaaatat aaaatgccct
      241 gaaggttcag agagagtagg cgattatcac actcacggtt tttactctga cgataaagga
      301 aataaagtaa caaaagaaaa tgatgtttat gatagtctaa atttttcaag caaagattta
      361 acgaattctt atatgaatgg aatgggaaaa aaagaataca gtagttactt gggaacacca
      421 aataacacct atctaaaata taatcccaaa gctaaaggga atggagttac aattatcagg
      481 caagggagta attaaaatga aggcaataca atgttgttat ttccttgctt tagtctccag
      541 tcatttaatg gctggagtta tgggtgatga taatatattt ttaagtaatt cggattcagc
      601 tgctttatat tgtgtggcta aaatatttcc agatggagaa aatattttaa tatataaagt
      661 aaatataatt aatgctagtt caaaaaaaga tgttttttta tgaaagccca acatcaaccc
      721 acaatgacat aatggggggc gggggaatgt catatgccta tgaatatgat gtaaaatata
      781 atatatgtca tttgagaaaa aagagcagaa tgaggtaaaa atctttggct gtgtattcag
      841 tctggtaatc atccaaggaa agcttatcag aagggtgatg actccaatta gtagaacata
      901 tattttcgat aaaagcaccg atgacttttt catgaagttc tacagaacgc gagaaacaga
      961 tggttctgcg ggccagacgt ttgattcggg ttctcagggt cagattgtta cgctcaatgt
     1021 gctgagtaaa aatcttacca gtcagatgct tctccttcgg taactctcta gcgtaactac
     1081 cccattcgtc agtggtcaac atgccgatgg taaaagggta agcagcgccc gcaattcacg
     1141 acaggtttcg tcagtccggg ggccaaaagt gtaggccaga acaccaccag ttttcgtgtt
     1201 gtacacgtac cagagccagt gctgtcgcgc tttactgccg acaaagctcc attgctgact
     1261 aagtttgcag atgagtgcca catcagcatg agcgaccggg gatgacgtta ttcttcgtgg
     1321 cgagagtttt ttaaagttcg gatgactgta ttaatgccga ttttcagcgt acgggcagta
     1381 tcgcgaaccc cggcaccgtt gaaagtcatt tcagcgattt ggtctttaac tcccggttta
     1441 cggggctcgt aacagtaact gagctgaaat acccggtgac agtcacggcc gcggaatcga
     1501 tcatgtcttt ttgggttctg cccgtgacgg taaacctgaa cggactggca acgggggcaa
     1561 tgaaggttaa ctgtgaccat gaaacaacct taaaagcgag tattatatcc ttaattcaac
     1621 taattggagg catcaccgtt taccggagcg acagcttgcg gaaaggtcgt gcaactacaa
     1681 agcaacaaac agactacggc gggaattatt tttttcatca tccattacca cgaaaacagc
     1741 ggccactctt tggcaaccgt ggtgagttgt ggcgcgtcga aaacggcttg tggtgaaact
     1801 accgtttcct caagcggaat atttttaccg tagcgttctt tgagcttcgc ctggaccagc
     1861 gggttgctga gatcaaactc tttcaacttc tgctgttcgc ccacgcgcat tcccagcgcg
     1921 ttctggtaaa ccttccacac cacttccgaa cagtactggc gatcgtcgct ccatgagaag
     1981 ctgaaatcat acggtttacc gagataacgt tttgccgttt gcgccagttt ttgctgttgt
     2041 tcaacactca gtccgccttc aacgcggcga acgacgtatt tgcccttttc accatgggca
     2101 atccactgct tgagcggggt gtatttcacc gggccgaccg cttcaaaaat gtagggcttt
     2161 ttgttacgca tcaccagcat accggtgtgg ctgtaatcgg aatgggttgc cagttggatc
     2221 gctttacttt gcgatgagcg agagatttga aagatgatgt cgccagtttg cggctgccag
     2281 gcaaaggcag gtacagaaac cagcaggctg aggatcagca gcctgttttt catagttaaa
     2341 cgtccatgta taaaaagcgg tgggtcgcag acaacgtgat cgttgtttat gccggatgcg
     2401 gcgtgaacgc cttatccggc ctacaagttc gtgcaaattc aataaattgc aatatgacgt
     2461 agatactgat aagcgtagcg catcaggcga ttccactccg cgccgctcat ttccgcttta
     2521 gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg ccgcttccgg
     2581 gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt gcccggtaaa
     2641 ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca ggttgttcac
     2701 ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa tggaaaccgt
     2761 aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga tatattcgcc
     2821 gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac cagaccacac
     2881 aatttatgtg ggtactgacc gtaaacccgc atagtttacc gtacaggcgt taccgtgaca
     2941 tcgtgtaatg cacctgtcgg cgtgataatg catataattt taacggctat ttgggatttg
     3001 ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat taacacaatg
     3061 tttactctgg tgcctgacat ttcaccgaca aagcccaggg aacttcatca catgacgcgt
     3121 aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta tggttgcgtt
     3181 gtcccaccga tccatctttc cagcacatat aactttaccg gatttaatga accgcgcgcg
     3241 catgattact cgcgtcgcgg caacccaacg cgcgatgtgg ttcagcgtgc gctggcagaa
     3301 ctggaaggcg gtgctggtgc agtacttact aataccggca tgtccgcgat tcacctggta
     3361 atgaccgtct ttttgaaacc tggcgatctg ctggttgcgc cgcacgactg ctacggcggt
     3421 agctatcgcc tgttcgacag cctggcgaaa cgcggttgct atcgcgtgtt gtttgttgat
     3481 caaggcgatg aacaggcatt acgggcagca ctggcagaaa aacccaaact ggtactggta
     3541 gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg ccatcaggca
     3601 agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc attacaaaat
     3661 ccgctggcat tgggtgccga tctggtgttg cattcatgta cgaaatatct gaacggtcac
     3721 tctgacgtgg tggctggcgt ggtgattgct aaagacccgg acgttgtcac tgaacttgcc
     3781 tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct gctgctacgc
     3841 gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc gcaacgcgca ggcgattgtg
     3901 aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct gccggaaaat
     3961 caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt gagttttgaa
     4021 ctggatggcg atgagcagac gctgcgtcgt ttcctgggcg ggctgtcgtt gtttacgctg
     4081 gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat gacacatgca
     4141 ggcatggcac cagaagcgcg tgctgccgcc gggatttccg agacgctgct gcgtatctcc
     4201 accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt ccgggctgca
     4261 aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt cagctgcata
     4321 aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg ggcattatgg
     4381 cggagtactc tcagcctgac gatatgatgg tggtttccgc cgccggtagc accactaacc
     4441 agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat caggttcaac
     4501 aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgct gaagaagccg
     4561 atagcctcat tagcgctttt gtcagcgacc ttgagcgcct ggcggcgctg ctcgacagcg
     4621 gtattaacga cgcagtgtat gcggaagtgg tgggccacgg ggaagtatgg tcggcacgcc
     4681 tgatgtctgc ggtacttaat caacaagggc tgccagcggc ctggcttgat gcccgcgagt
     4741 ttttacgcgc tgaacgcgcc gcacaaccgc aggttgatga agggctttct tacccgttgc
     4801 tgcaacagct gctggtgcaa catccgggca aacgtctggt ggtgaccgga tttatcagcc
     4861 gcaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat tccgcgacac
     4921 aaatcggtgc gctggcgggt gtttctcgcg taaccatctg gagcgacgtc gccggggtat
     4981 acagtgccga cccgcgtaaa gtgaaagatg cctgcctgct gccgttgctg cgtctggatg
     5041 aggccagcga actggcgcgc ctggcggctc ccgttcttca cgcccgtact ttacagccgg
     5101 tttctggcag cgaaatcgac ctgcaactgc gctgtagcta cacgccggat caaggttcca
     5161 cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc agccacgatg
     5221 atgtctgttt gattgagttt caggtgcccg tcagtcagga tttcaaactg gcgcataaag
     5281 agatcgacca aatcctgaaa cgcgcgcagg tacgcccgct ggcggttggc gtacataacg
     5341 atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg ctgaaaatcc
     5401 tcgacgaagc gggattacct ggcgaactgc gcctgcgtca ggggctggcg ctggtggcga
     5461 tggtcggtgc aggcgtcacc cgtaacccgc tgcattgcca ccgcttctgg cagcaactga
     5521 aaggccagcc ggtcgaattt acctggcagt ccgatgacgg catcagcctg gtggcagtac
     5581 tgcgcaccgg cccgaccgaa agcctgattc aggggctgca tcagtccgtc ttccgcgcag
     5641 aaaaacgcat cggcctggta ttgttcggta agggcaatat cggttcccgt tggctggaac
     5701 tgttcgcccg tgagcagagc acgctttcgg cacgtaccgg ctttgagttt gtgctggcag
     5761 gtgtggtgga cagccgccgc agcctgttga gctatgacgg gctggacgcc agccgcgcgt
     5821 tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc ctgtggatgc
     5881 gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag cagcttgctg
     5941 atcagtatct tgatttcgcc agccacggtt tccacgttat cagcgccaac aaactggcgg
     6001 gagccagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa accgggcgtc
     6061 actggctgta caatgccacc gtcggtgcgg gcttgccgat caaccacacc gtgcgcgatc
     6121 tgatcgacag cggcgatact attttgtcga tcagcgggat cttctccggc acgctctcct
     6181 ggctgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat caggcgtggc
     6241 agcagggctt aaccgagcct gatccgcgtg acgacctctc cggcaaagac gtaatgcgca
     6301 agctggtaat tctggcgcgt gaagcgggtt acaacattga accggaccag gtacgtgtgg
     6361 aatcgctggt gcctgctcat tgcgaaggcg gcagcatcga ccatttcttt gaaaatggcg
     6421 atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg gggctggtgc
     6481 tgcgctacgt ggcgcgtttc gatgccaatg gcaaagcgcg tgtgggcgtg gaagcggtgc
     6541 gtgaagacca tccgctggca tcactgctgc catgcgataa cgtctttgcc atcgaaagcc
     6601 gctggtaccg cgataatccg ctggtgatcc gtgggcctgg cgctgggcgc gacgtcaccg
     6661 ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt cagaaattta
     6721 ataatgcccg gtactcatgt tttcgggtct atggtttcta atgaaatata ttgaattatc
     6781 ataggattag gccggatgaa gcgtttacga caaatccggc aagaagcaat aagtacatgg
     6841 ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt cttaccatcc
     6901 tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt tcggttgacg
     6961 cccttcggct tttccttcat ctttacatct ggacgtctaa atggatagat gtgcacaaca
     7021 caacatataa ctacaagcga ttgatgaggt aaggtatgag cttttttcac gccagccagc
     7081 gggatgccct gaatcagagc ctggcagaag tccaggggca gattaacgtt tcgttcgagt
     7141 ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc gatcgcctta
     7201 gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc gagcgcgacc
     7261 gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg gcaccgcatc
     7321 ttacttgcat tgatgcgacg cccgacgagc tgcgcaccat tgcacgcgac tactggaata
     7381 acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt ggtaagccgg
     7441 aaatgtatgc ttctgacctg gtgacgctgt taaaagaagt ggcagatttc gatatctccg
     7501 tggcggcgta tccggaagtt cacccggaag caaaaagcgc tcaggcggat ttgctcaacc
     7561 tgaaacgcaa agtcgatgcc ggagccaacc gcgcgattac tcagttcttc tttgatgtcg
     7621 aaagctacct gcgttttcgt gaccgctgcg tatcggcggg cattgatgtg gaaattattc
     7681 cggggatttt gccggtatct aactttaaac aggcgaagaa atttgccgat atgaccaacg
     7741 tgcgtattcc ggcgtggatg gcacaaatgt ttgacgggct ggatgacgat gctgaaacgc
     7801 gcaagctggt gggcgcgaat attgccatgg atatggtgaa gattttaagc cgtgaaggag
     7861 tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg atttgccata
     7921 cgctgggtgt tcgacctggt ttataaatag tgtggctttt gtgaaaatca cactgtgatc
     7981 acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgtaaaa ataaggttgt
     8041 cagctttgtt ttcgccctcc ttccttgaag aataggaatc taaaaccctt ttttataaag
     8101 cagttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt gatctacatc
     8161 tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat tcaattataa
     8221 cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac attgatgagc
     8281 acgtcagacg atattcataa cacgacagcc actggcaaat gcccgttcca tcagggcggt
     8341 cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa tcaactccgt
     8401 gttgacctgt taaaccaaca ttctaatcgt tctaacccgc tgggtgagga ctttgactac
     8461 cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa agccctgctg
     8521 acagaatctc agccgtggtg gcctgccgac tggggcagct acgccggtct gtttattcgt
     8581 atggcctggc acggcgcggg gacttaccgt tcaatcgatg gacgcggcgg cgcgggtcgt
     8641 ggtcagcaac gttttgcacc gctgaactcc tggccggata acgtaagcct cgataaagcg
     8701 cgtcgcctgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg ggccgacctg
     8761 tttatcctcg cgggtaacgt ggcgctggaa aactccggct tccgtacctt cggttttggt
     8821 gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga tgaaaaagcc
     8881 tggctgaccc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc aaccgagatg
     8941 ggcctgattt acgttaaccc ggaaggcccg gatcacagcg gcgaaccgct ttctgcggca
     9001 gcagctatcc gtgcgacctt cggcaacatg ggcatgaacg acgaagaaac cgtggcgctt
     9061 attgcgggtg gtcatacgct gggtaaaacc cacggtgccg gtccgacatc aaatgtaggt
     9121 cctgatccag aagctgcacc gattgaagaa caaggtttag gttgggcgag cacttacggc
     9181 agcggcgttg gcgcagatgc cattacctct ggtctggaag tagtctggac ccagacgccg
     9241 acccagtgga gcaactattt cttcgagaac ctgttcaagt atgagtgggt acagacccgc
     9301 agcccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat cccggatccg
     9361 tttgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac gctgcgtttt
     9421 gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc gttcaacgaa
     9481 gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa atctcgctac
     9541 atcgggccgg aagtgccgaa agaagatctg atctggcaag atccgctgcc gcagccgatc
     9601 tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga ttctggtctg
     9661 tctgttagtg agctggtatc ggtggcctgg gcttctgctt ctaccttccg tggtggcgac
     9721 aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga ctgggatgtg
     9781 aacgccgcag ccgttcgtgc tctgcctgtt ttggagaaaa tccagaaaga gtctggtaaa
     9841 gcctcgctgg cggatatcat cgtgctggct ggtgtggttg gtgttgagaa agccgcaagc
     9901 gccgcaggtt tgagcattca tgtaccgttt gcgccgggcc gcgttgatgc gcgtcaggat
     9961 cagactgaca ttgagatgtt cgagttgctg gagccaattg ctgacggttt ccgtaactat
    10021 cgcgctcgtc tggatgtttc caccaccgag tcactgctga tcgacaaagc ccagcaactg
    10081 accctgaccg cgccggaaat gaccgcgctg gtgggcggga tgcgtgtact gggtgccaac
    10141 ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag caatgacttc
    10201 ttcgtgaact tgctggatat gcgttacgag tggaaagcga ccgacgaatc gaaagagctg
    10261 tttgaaggcc gtgaccgcga aaccggcgaa gtgaaataca ctgccagccg tgcggatctg
    10321 gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag tttacgccag cagcgatgct
    10381 cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa cctcgaccgt
    10441 ttcgacctgc tgtaatctga ccttcttcag cgactgcctt tcaggcagtc gctgaagttt
    10501 ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat gtctgtcgca
    10561 ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat ctggagttat
    10621 agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt taccgcctta
    10681 cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg tcgcggaatg
    10741 cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg cgggatggtt
    10801 ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat cctaagctat
    10861 accatgccgt tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg cctgcgacgt
    10921 gggcaatatt tcgcgatact gattgccgct ttcggcttat ttttggtgtt gcagccgtgg
    10981 caactcgatt tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg cgtcagttgg
    11041 ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt ggatttattg
    11101 tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt cgctttactg
    11161 gtgccgcaac gtgaaattga ctggcagccc accgtgttct gggcgctggc ctacagtgcg
    11221 attctggcga cggcgctggc gtggagctta tggttgtttg tattgaaaaa cttgcctgcc
    11281 agtattgcca gcttaagcac gctggccgtt cccgtttgcg gcgtactctt ttcctggtgg
    11341 ctgctcggtg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat tgtgctggca
    11401 ctggcgctgg tgagccgtaa gaaaaaagaa gctgtcagtg taaaaagcat ctgaattttt
    11461 tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac catccgccag
    11521 ctaaacagca catcttcttc ctgcgcgcct gcgccaatgt tatgtatcac cagcggcgta
    11581 ccgtcgcggg cgaagccatc tgacaccacc ccaatatgtg ccagtccgtt atccagtcgc
    11641 caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg gcgtgtttta
    11701 tcgtggcggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat gttgctgtcc
    11761 gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat atcttcgtgc
    11821 accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac atcggaacat
    11881 accccgcgtt cttgcgggac atcaccgcca ggataggtaa gctgcatata cgccggatcg
    11941 taaaatagcg tgctgccaat ttgctgtctg gcaccgtctg cgatggcaag gttggtattg
    12001 gcctggattt gtaccacggt tggtggaacg gcgggagatt ttaaggagtg gctggtaaat
    12061 gccgttagca ggctgagcag cgccagtgaa gctttcatct ggttcgtccg tgaaaataag
    12121 agtcacagat tcgaccttcc gggctaacga ttgttctgta gagaaaatgt ccagaagcgg
    12181 ataaaaaact cctgaacaac gtaggagttt cagattactg gcacagagtg ccggatgcgg
    12241 cgtgaacgcc ttatctggcc taaaaagcac gcaaattcaa tatattgcag agattgcgta
    12301 gatcctgata agcgtagcgc atcaggcaat tttgcgttca aactcccgga caagccggga
    12361 gtttggagta ggttattccc actcttgcag gaaacgctga ccgtactggt cggctaccag
    12421 cagagcggcg taaacctgat ctggcgtcgc gccgccaggc atgttgtgga tggtttcacc
    12481 ttctgcacat gccgcttctg ccacaattcg cattttcgcc gggacatctt ctttaatatc
    12541 cagttgagcg agagttattg gcaaacctac cgcatggcta agtgcagcta cggtttcgat
    12601 ttcctcaacc ggcgcgtttt ccagtaccag ctgcgtcagc gtaccgaatg ccactttttc
    12661 accgtgataa tagtgatgcg cgtccgggat agcggtcagg ccgttatgca ccgcatgagc
    12721 cgccgccagt ccgccgcttt caaaaccaac gccgcttaaa taggtgttcg cttcaatcac
    12781 gcgttccagc gcaggggtta ctacatgctg ttcggcggca agcatcgctt tttcgccttc
    12841 ttccagcaga gtgttgtagc acagttctgc cagtgccagc gccgcctggg tgcacttgcc
    12901 gcccgccata gtggtagcgc cgctacggga acaggcgcgt gcttcaaacc aggttgccag
    12961 cgcatcgccg atacccgccg ctaacagacg tgcaggagcg cccgcgacga ttttggtgtc
    13021 gacaatgacc atattcgggt tatttggcaa cagtagatag cggtcaaact caccctcatc
    13081 ggtgtagata acagacaatg cgctgcacgg tgcatcggta gaggcgatag tcggtgcgat
    13141 cgctaccgga acacccatga aatgtgccag tgctttggca gtatcgaggg tttttccgcc
    13201 accgataccg agaattgcgc cacactgcgc agtctccgcg atgccacgca gacgatcgat
    13261 ctcattttgc gaacattcac cgccaaacgg cgcaatttct actaccagtc cagcatcttt
    13321 aaagcttttc tcgacagtgg attgagcaaa acctaaaaca aatttgtcac ccaccactaa
    13381 ccagcgttct gccagcggct tcaggtattc gcccagacga ttaatcacat cagcgccctg
    13441 gatgtattta cccggtgatt gaataatgcg gtccataatt gctcctttag agatgagtag
    13501 tgccaaatgc ggcattccag tcgtgttcga acttctctat agctgactct accgcagggg
    13561 tgttgagcat ttgttgcgct acatctaaag gcagggtgat ggactcacat cctgccagca
    13621 aacaatccag tgcctgacgc ggcgttttaa agctggctgc aagcaccatg ctttctggcg
    13681 cgtgcatttc taacagcgct tgcagctcct gaaccgtacg aatgccgtcc ccgccctggg
    13741 catctacgcg gttaacataa ggagcaacgt attttgcccc tgccagcgcg gccagtaacc
    13801 cttgtgcagc actgtacact gcggtgccaa gcgtagtaat accctctttt ttcagcatct
    13861 taatggcggc cagaccttcg gaagtcaccg ggattttcac cacaatgccc ggaatggcgt
    13921 cgcgcaggcg cttcgcttct tccaccatac cctgcgcgtc acgactcata gtctgagcaa
    13981 acagaatgcc ctcatcacca atcgcttttt gcagacgcgg cagaacttcc catatggact
    14041 ccttgctggc agcgataatg ctcgggttag ttgtcacccc ggcaatgggg aatatgcgtg
    14101 ccagacgttc gacttctgcg acgttagcgg tgtccagata cagttccatg atgtttcctt
    14161 ttacagttcc agttcatgtt gcagcaggct ggcgatagcg tcggcagagg cggcgttaac
    14221 cagcgcgtta cggaattctt cgtgcatgat gcggcgagcg aggcgcgaga aaatgcgcat
    14281 gtgctgatcg cccgcagcgt gtttgttcag ggttaacata atgatgaatt gcgcttcatc
    14341 atcgccccag cgcaccggcg cttgcagacg cgctacactg atggtggatt gctcaatgtg
    14401 ttctgatttg ctgtggggaa tggcaaaact aaagcccaga ccggtagaga aaacggcctc
    14461 gcgcgcccac aagtcggctt ccagtttacg cggatagcgg cagcggcccg ccagcagcag
    14521 gttatcggtc atgcctttga gcacttcttc tttgctgcgc cagtcgcttt ccagcgtgat
    14581 gcactcggcg gtgaccagcg gtgcgtcctg ttgggtcatg cggaattgcg ccagcaggtg
    14641 ttccacttcc agcgaagtac ggcaggccat tgcctggttg agcaactggc ggcattcgcg
    14701 gctatcaagt tgcgccattc gcgctttcgc cgccggaatt gacggtgcgc tcatgctcag
    14761 ctcatccagc cctaaaccga ccagcaacgg cagcacggaa cctttcgctc ccagctcacc
    14821 gcacagacca atccatttgc cctggcgatg caccgcttgc acggcgtaat cgagcgcccg
    14881 caagaatgcc ggattcaggc tgttgtagtg acgagtaacc ttagcattat cgcgatccac
    14941 cgccagcaga tattgcgtca ggtcattact accaatacta aagaaatcaa tctcttcgca
    15001 gcattgatcg atgatgaaca tcaccgacgg cacttccagc ataatgccga gctggatctt
    15061 ctcatcaaac ggaatgtgtt cgttacgcag ttgctgtttg gcttccgcca gtttttcttt
    15121 cacccataag atctcttcca ttgaagagat catcgggatc atgattttca ggctgccgtg
    15181 agcggaggcg cgcaggatcg accgtagttg tgtggtgaac agcgaggcgt attcttcata
    15241 aatacgcaca gcgcgataac cgaggaacgg gttagcttct gccggaatgt tcagataatc
    15301 aacgggtttg tcgccgccaa tgtccatagt gcgcacaata atgctgcgtc cgttggcaga
    15361 ctccagcgcc tgacaaaaaa tgttgtacag ctcgctttcg cccggtgcac tggtgcgatc
    15421 catatagagc atttcagtgc ggaacaaacc aacgccttcc gcactattgc cgaatgcggc
    15481 ctgcgcttcc acggagtgag cgatgttagc ggcaatttca atgcggatac cgtcagcggt
    15541 atgggcttgt tgggtcagcc agacacgctg ttgctcacgc agggcatcct gtacgcgggc
    15601 ttcttgctga taataacggg ctacggcttc ccctggctca accacaatcg ctccggcgtt
    15661 gccgtcgata taaatcgttt gatgttgcca cggggtaagg gcatcaatat ccacaccaac
    15721 cagcatcgga atgttgaacg aacgggcaag gatcaccgta tgtgaggtgg tgccgccgct
    15781 tttgagcaac aatcctttga ggtgattttt atccagttcg aggaactggc tgggggtcag
    15841 ttcatcagcc atacaaatgg cgggctgcgt cagtttgccc ggtgccggga agcgttgctc
    15901 accgtagatt tgctggagta actggaagca gacgtcgcgt acgtccaggg cacgttcttg
    15961 caggtagctg ctgctggaac gggaaaactc ttcacaaaag tgattcgcac tggcgacaat
    16021 cgcttcggcg cagcttaatc cggcgctgac gcctgccagt aaatgttcgt gcagggaagt
    16081 atcgccagcc agggatcggt gcgcttccag aatcgcgctg gtggcaccgt cgctatccag
    16141 cagacgaaac tcaatgtttt tcagtaccag cgtcaggccg ttttccagtg cggattgctc
    16201 ggcgtcaacg tctttggctg cgggaagatt actcagcgca ttgagatcta aagaagagat
    16261 cggcgtcaga atgccgcctg cgctaccgct gcacacggtg cgggcgcgga taatttgcgg
    16321 attcagattg gtcagtgaaa ccggcagtgg ttccagttcg tcagatttaa cttccgccag
    16381 cggtgcgtcg cagtggggga attcatcgcg cagccattgg cttaaacgct ggtgcgcttc
    16441 ctgttcgtcg gccccggaaa tcagtaaccg gcagttatcg cccgccagcg tgtcggtgcc
    16501 aatcagcgca agggcgcttt tggcgttgcc cttgcggtca gtgcgcaggt tatgccactc
    16561 aatttgtgat gaaaaagtat tacacagcgt ttcaacgtgg cttgccggac gtgcatgtac
    16621 gccgttaggt agctcacaaa taaattccac aatcagggcc attgcctctc tcccggtacg
    16681 gttttctgtt tacaggataa gagcgcacgg caacggcctg ccatgtgaca aatctgccaa
    16741 aagctggaca aatgtaatgt aaccgtcaat ttgcgacgcg tctcacaaga cgccgttttg
    16801 cggcatgctt ccggattatc gcaagttatg aggcggatcg catttttgta ctgatattac
    16861 aaaagtccag taaatggcct ttttatccac tgtttgctcc gcctgcgatt gcctattgtt
    16921 tcctccatca aaaatatgga catgggcttt cgcaagcccg ggagcagctt atgaatgagt
    16981 tggtgcagat cctgaaaaat acccgtcagc atttaatgac gggcgtttca cacatgattc
    17041 ccttcgtggt atcgggcggt attttgctgg cggtttccgt catgttgtat ggcaaaggcg
    17101 cagtgccgga tgccgtagcc gatccgaatc tgaaaaaact gtttgatatc ggagttgcag
    17161 gtttgacgct gatggtgcct ttcctcgccg cttacatcgg ttactccatt gcagagcgtt
    17221 ctgcgctggc cccgtgcgct atcggtgcct gggttggtaa cagctttggt gcgggcttct
    17281 ttggtgcgct gatcgccggg attatcggcg gcatcgtggt gcattacctg aagaaaattc
    17341 cggtgcataa agttctgcgc tcggtgatgc ctatcttcat tattcctatc gtcggcacac
    17401 tgattaccgc aggcatcatg atgtggggct tgggcgagcc tgtaggggcg ttgaccaaca
    17461 gcctgactca gtggcttcag gggatgcagc agggcagcat tgttatgctg gcggtgatca
    17521 tgggtctgat gctggcgttc gatatgggcg gtccggttaa caaagtggcc tatgccttca
    17581 tgctgatttg cgttgctcag ggtgtttata ccgtggtggc cattactgcc gtgggtattt
    17641 gtatcccgcc gctggggatg gggctggcga cgctgattgg tcgtaaaaat ttctccgcag
    17701 aagagcgcga aaccggcaaa gcggcgctgg tgatggggtg cgttggggtt actgaagggg
    17761 cgattccttt cgccgctgcc gatccgctgc gtgtcattcc ttccatcatg gtcggttctg
    17821 tttgtggtgc ggtaactgcg gcgctggtcg gtgcgcagtg ctatgcaggc tggggtggtc
    17881 tgattgtgct gccggtggtt gaaggcaagc tgggttatat cgcagcagtg gctgtcggag
    17941 cagtggtgac ggctgtttgc gtgaacgtgc tgaaaagtct ggcgcgtaaa aatgggtctt
    18001 cgactgatga gaaggaagac gacctggatt tggattttga aattaattaa ttgaggaacg
    18061 agccatgacg aaaattattg cagtaaccgc gtgtccatct ggtgttgccc atacctatat
    18121 ggcggcagag gcgctggaaa gcgccgcgaa agcgaaaggc tgggaagtga aagtagaaac
    18181 tcaggggtca attggtctgg aaaacgaact gactgcggaa gacgtggcga gcgccgatat
    18241 ggtgattctg accaaagata tcggcatcaa gtttgaagag cgttttgctg gcaagaccat
    18301 cgtgcgcgtc aacatcagcg atgcggtaaa acgtgctgac gccattatga gcaaaattga
    18361 agctcacctg gcgcaaaccg cttaagttct tctcccgctc gcaagggcgg gttcgctttc
    18421 ccacaggagt tcctcatgac gaatcgtatc tctcgcctca aaactgcact gttttccaat
    18481 acccgtgaaa tctcgctgga gcgggcgctg ctttataccg ccagccatcg gcaaaccgaa
    18541 ggcgaaccgg tgatattgcg ccgggcgaaa gcaacagcgt atatccttga acatgttgaa
    18601 atttcgattc gtgatgaaga actgattgcc ggtaaccgca ccgtaaaacc gcgcgccggg
    18661 attatgtcgc cggaaatgga cccttactgg ctgctgaaag agctggatca attcccgacg
    18721 cgtccgcagg accgctttgc tatcagcgaa gaagataaac gtatctaccg cgaagagttg
    18781 ttcccgtact gggaaaaacg ttcgatgaaa gatttcatca acgggcagat gacagatgaa
    18841 gtaaaagccg cgaccagcac gcagattttc agcattaacc agacggataa aggccagggg
    18901 cacattatta ttgattaccc acgcctgctg aatcacgggc tgggggagct ggtggcgcag
    18961 atgcagcaac attgtcagca acagccggag aatcactttt atcaggccgc gctgttactg
    19021 ctggaagcct cgcagaaaca cattctgcgt tacgctgaac tggcggaaac aatggcggca
    19081 aactgcacag atgcccagcg tcgcgaagaa ctgctgacta ttgcggagat ctcccgccat
    19141 aacgccgaac ataagccgca gacgttctgg caggcgtgcc agttattctg gtacatgaac
    19201 atcattctgc aatacgaatc caacgccagt tcgctgtctt tagggcgctt cgaccagtat
    19261 atgttgccgt tctatcaggc ttctttaacc cagggcgaag atccggcgtt cctgaaagaa
    19321 ctgctcgaat ctttatgggt gaaatgcaac gacatcgtgc tgttgcgctc caccagcagc
    19381 gcgcgttatt tcgcaggttt cccgaccggc tataccgcac tgctcggcgg gttaaccgag
    19441 aacggacgta gcgcggtgaa cgtgctttcg ttcctttgcc ttgacgccta tcaaagcgtg
    19501 caattaccgc aaccgaacct cggcgtgcgc actaacgcct tgatcgacac gccgttcctg
    19561 atgaaaaccg ccgaaaccat tcgcctcggc accggtattc cgcaaatctt taacgatgaa
    19621 gtggtggtac cagcgttcct caaccgtggc gtttcgctgg aagatgcgcg cgactattcc
    19681 gtagtgggct gtgtggaatt atctattccc ggcagaacct acggcttgca tgacatcgcg
    19741 atgtttaatc tgctgaaagt gatggaaatc tgcctgcatg aaaatgaagg caatgctgcg
    19801 ctgacttatg aaggtttact ggagcagatc cgcgccaaga tcagccacta catcaccctg
    19861 atggttgagg gcagcaatat ttgtgatatc ggccatcgcg actgggcacc tgtaccgctg
    19921 ctctcgtctt ttatcagcga ttgtctggaa aaaggtcgcg atattaccga tggcggcgcg
    19981 cgttataact tctccggcgt acaggggatc ggtatcgcca acctgagcga ttctctccat
    20041 gcgttgaaag ggatggtttt tgagcaacag cgtttaagtt ttgacgaatt gctgtcggta
    20101 ttaaaagcca actttgcaac gccagaaggc gaaaaagtcc gtgctcgctt aattaaccgc
    20161 ttcgagaaat acggtaacga tatcgacgag gtggataaca tcagcgccga actgttacgc
    20221 cactactgca aagaagtgga aaaataccag aacccgcgcg gtggttactt cacgccggga
    20281 tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac gccagacggt
    20341 cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg ccaggacgca
    20401 caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct gctgtctaac
    20461 ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc gggattacgc
    20521 aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat tcagtttaac
    20581 gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta tgccgggctg
    20641 gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga gatccaggat
    20701 gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc gcaatgatgt
    20761 ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca ttcgtacggt
    20821 ggtctttttt aaaggctgtc cgcatctttg cccatggtgt gctaatccgg agtcgatctc
    20881 cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga aatgtttgcg
    20941 tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata tcagccttga
    21001 cgcgctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg gcggcggcgt
    21061 cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt ttttacagcg
    21121 gctgcggttg tggggcgtgt catgcgccat tgaaactgcc ggagacgcgc cagccagcaa
    21181 gctgttacca ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa ttatggacgc
    21241 gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga atctgcgttt
    21301 gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg gtttcacgct
    21361 cagccgggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa tcaggcagat
    21421 ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg ggaaaacatg
    21481 gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc gcgaaatggc
    21541 agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg gtggcagtaa
    21601 ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg gaaaagttgt
    21661 gccagttaga gaagtggggc gttagcattg aaactcaggg cgcgctggga acggagaatc
    21721 gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg gatatcgagc
    21781 ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc tacgcattcc
    21841 tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg ccgcagcaaa
    21901 cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact gccgacgata
    21961 ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt tgctgtcgac
    22021 aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca ataacgtctt
    22081 agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc ccgttttttg
    22141 aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag attcgcgggt
    22201 aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata gtgcctggct
    22261 gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa gcaggctggc
    22321 gacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat tgagcgtttg
    22381 tagcagaaaa gaacctatgc gtgggccgcg ccgggcgacg tgttgcttcg ccagattttg
    22441 atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc caaacagcac
    22501 gctaaaggta gtagcggggg cttgccattg tggaaaattc cagccaccag caggaacgta
    22561 cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact caccttccag
    22621 cacaatttcc agccgtggaa aatcgacctg atacgcgaga tcgggcaccg ggccgttact
    22681 gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg agagcagata
    22741 gctgacgtcg tgatacatgt tattccctta ggtatgtaga cgggaaaaca gtaactggca
    22801 ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta aacattgcgg
    22861 cagtatcgca tccggcaaat agcgccagac gcgatacaaa ggtcatttac tgattaccca
    22921 cctgatcgcc atacggcagc gtgtcgtaat cgatcagcgc gtttttcttg tacgggttac
    22981 caatccagcg ggtagtttct ttgaactgcg gattcaccac tgaacgggtt ggatcgtaac
    23041 cgtcgtaaga taagcctgct aaatctgacc aggtgtggat cagttccgcc aggctgtatt
    23101 tacgatcaac atcctgcgag aaatcacgcg gatgagtcgc ttgccatttt tccgacgtcc
    23161 acagcaggaa cggaatggtg tacatgtggc gcgtcgggtt gtcttcgtta cgcccctgag
    23221 ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc agagaaataa accaggaaac
    23281 cgttcgggtt tgctgcttta aagtctttaa tcaggctggc aaccacatga tcgttataca
    23341 ggttagcgtt gtcataatcg ttatatgact ccagctcttc cgcgtttaat ccaggcggaa
    23401 catgatcggt attgccatca aacttgccct gattttccgg gtagcggtat ttgtatttga
    23461 tatgcgtacc cagcagatga acgatgatca gtttcttcgg cgccgggtcc ttcagcactt
    23521 cctggaatgg tttcagcacg ttggtgtcgt attcacgcgc actctgcgta cgttgctggt
    23581 tcatatagaa ctgtttgtcg gtctgcttcg aaaataccgt cagcatggta ttgcgggcgg
    23641 tcatcgtctg ctggttggtg atccagaagg ttttataacc tgcctgtttc atcatgttca
    23701 tcagcgacgg ttgtgtcagg tacaggtccg ggttcttttc attagcaaag gtcagcgcct
    23761 gttgcaggat ttcaatggtg tacggacgag acgtaaccac gttattaaac acggtcagat
    23821 tcggatcggt tttatgcagc gcatccagct ccggcgtggt ttcacgcgga taaccgtaca
    23881 gactcatgcg cccgcgttgg gtcgattcgc caatcaccag cactaaggta cgcggttcgt
    23941 tacccgattc atctttgaaa ttagccagag gcggcaaggc attattttca ttcagtaact
    24001 ttgttagcga gtttagttgc tgacgatact gataatagcc ggtcaggaat tgccacggtg
    24061 cggcaggctc catacgcgag gccaggttat ccaacgtttt ctcaaacggc ttgtttttga
    24121 taaacgtatt catggcgatc ggatgcagaa tcaagccata aagcagggca aaagagacaa
    24181 cataacgcca cggctttgga atatagaccg ggtgcaggcg tgtccacagc agaactgcca
    24241 ccgccgtata ggccagcgcg ataagcacaa ttttcaggct gaaatactgg cttaaatact
    24301 cgctggcttc gttggtgttg gtttcgaaca tcacaaacag aacgctctgc gagaactcct
    24361 gcccgtagat gacgtagtag catagcgccg ccagagaggc cgcccatagc accacgccga
    24421 ttactgcggc aataatttta atccgcttcg gaaagaggaa taccgggatc aaccacagcg
    24481 agctgaataa cagcgagtcg cgaatgccgt tggtgccact ataaccactg atgtaaataa
    24541 tggcctgtag cagagtagag aaaaaccaaa agtagagcag tgcccaaccc agggctttcc
    24601 agctaaaaag gggtttagcc tggacttctg tggaatgcat agtaagaacc tgtcttgaaa
    24661 aaaatatcgc cgaatgtaac gacaattcct taaggatatc tgaaggtata ttcagaattt
    24721 gaataaaatg cagacagaaa tatattgaaa acgagggtgt tagaacagaa gtatttcaga
    24781 aaaccctcgc gcaaaagcac gagggtttgc agaagaggaa gattagccgg tattacgcat
    24841 acctgccgca atcccggcaa tagtgaccat taacgcttgt tcgacgcgag gatccggttc
    24901 ctggccttct ttttctgcct ggcgggagcg gtgcagtaac tcggcctgca atacgttcag
    24961 cgggtcggtg taaatattcc gtagctgaat agactctgca atccacggca gatcggccat
    25021 tagatgggaa tcgttggcaa tcgccagcac cactttgatg tcttcttctt gcaggttgcg
    25081 taactcttta cctaacggcc acagtgcttt gtctaccagg cgttggtcat agtattccgc
    25141 cagccacagg tctgctttgg cgaagaccat ctccagcatg ccgagacgcg tcgagaagaa
    25201 tggccaatcg cggcacatgg cttccagctc gctctgtttg ccatcttcga ccactttttg
    25261 cagcgccgta cctgcaccca gccaggcggg gagcatcagg cggttttgcg tccaggcaaa
    25321 aatccacggt atggcgcgca gcgactcgac gccgccggtt gggcgacgtt tcgccggacg
    25381 tgaacccaac ggcagtttgc ccaattcttg ttccggcgta gcggagcgga agtaaggcac
    25441 aaaatctttg ttttcacgta cgtagccacg gtagagatcg caggagatga ctgacagttc
    25501 atccataatg cgacgccagc tctctttcgg ctcaggtggt ggcagcaggt tggcttccag
    25561 aatagccccg gtgtacagcg acaggctgct gacggtgatt tctggcagac catatttaaa
    25621 gcggatcatc tcgccctgtt cagtcacgcg caggccacct ttcaggcttc ctggcggttg
    25681 tgacagcagc gccgcatgag caggtgcgcc gccgcgacca atggaaccgc cgcgaccgtg
    25741 gaacaacgtc agctcaatac ccgctttttc gcaggttttg attaatgcat cctgtgcctg
    25801 atattgcgcc caggaagctg ccatcactcc cgcatctttt gctgagtcgg aatagccaat
    25861 catcaccatc tgtttgccct gaatcaggcc acgataccag tcaatattga gcagctgggt
    25921 catgacatcg ttggcgttgt tcagatcatc gagggtttca aacagcggag caaccggcat
    25981 cgcaaacccg atacccgctt ctttcagcag caggtggaca gccagtacgt cggacggcgt
    26041 tttcgccatc gagatcacgt aggcggcaat ggagccttgc ggtgcttcgg caatcacctg
    26101 gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc cagttgcgcg gcagaagcgg
    26161 acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg tcggcctctg accagctttc
    26221 gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc gcttcggtat gacgcgtgct
    26281 ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa catttcacgc ggcgcagggt
    26341 gtcgagcaga tcgccgttgg cgataatacc catgccacac gcctgaagtg actggtagca
    26401 agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc aggccttctg gttttggcag
    26461 ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc gccatcaggc gagaacgcag
    26521 gtttttcatc agatagcgat acggttctgc ggcaccttct tcgccaacca gcgccagcag
    26581 ttcaggggtc gcttcaacca tcgacagttc agaaaccagc acctgaatat ctttcaggaa
    26641 caaatcggtg gctttccagc ggctgagtag caggacgtgg cgggtgatat cggcagtgac
    26701 gttcgggttg ccgtcgcggt cgccgcccat ccacgaagta aaacggaccg gaacaaattc
    26761 gacgggcagt ttgtagccga ggttctcttc cagttgttcg ttcagttcgc gcaggtaatt
    26821 tggtacgcct tgccacaggc tgttttccac tacggcaaag ccccatttgg cttcatctac
    26881 cgggcttgga cgcagcttac ggatttcatc ggtatgccat gactgggcga ttaactggcg
    26941 caggcggcgc atcagctggt tgtgttcgta gtcagcgata tctttgttat cgagctgttt
    27001 taaacaggcg ttcacttcca ccattttgtg gatcagtgta cgacgggtaa tttcggttgg
    27061 gtgagccgtg aggaccagtt ccagcgacag cgattccact gcttttttga tggtgtcttc
    27121 gctcagttcc ggctggtttt tcagtttacg cagggtgcgg gcgatcactt ccgggttgct
    27181 ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg gcggtgttgg ccaggttcag
    27241 gaactgacta aacgcacgcg caacgggcag cagctcgtcg ttcgacaaat tttgtaaggt
    27301 ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt gaagatttcg acaacttacg
    27361 gatagtttct acgcgttcaa gaatgtgttc tcccaacgca tccttgatgg tttctcccag
    27421 cactttgccg agcatactga cattactacg caatgcggaa tattgttcgt tcatattacc
    27481 ccagacaccc catcttatcg tttgatagcc ctgtatcctt cacgtcgcat tggcgcgaat
    27541 atgctcgggc tttgcttttc gtcgtctttt ataaagccac gtaaaagcgg tgacgtcaaa
    27601 tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa tttacgccat taaattcacg
    27661 acgctttaaa taagcgtaac ttatggaaat gttaaaaaat cgccatacat aacaccaaag
    27721 gtgtaggtcg gataagatgc gcaagcatcg catccgacat tattgcggca caggagtttg
    27781 gcaacggtgc cggatgcggc gcgagcgcct tatccggcct acgttttaat gccagcaaaa
    27841 atggtgaatt acctgggtta tcagttcgcg ggtgggcttg ataaaccgtg tttccagata
    27901 ttcatcaggt tgatgagcct gattaattga gccaggcccc aacaccagcg tcgggcataa
    27961 cgtttgaata aacggcgctt cggtacagta gttcaccact tcggtctttg ctccgagcaa
    28021 tttctcaact acttcaacca gttgatgatt cggtgggcat tcatagccag ggatcggcgg
    28081 atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg ctcaccggag ccaatgcatc
    28141 gttgagcaaa ccattaagtt cattgagtgt catgccaggc agcggacgaa tatccatatg
    28201 caactcacag caagcgcaaa tacggttaga agcgtcgcca ccgtgaatat gcccgaggtt
    28261 gagcgtaggg tatggcacgg taaacgcttc gtagtgataa cgttctttca ggttatcgcg
    28321 caattgcaaa atatgcccga tggcgtcgtg cattagttcg atagcgttaa ctccgcgtgc
    28381 tggatcgctg gagtgccccg actggccctg aatacggatg gcgttagaga tatgaccttt
    28441 atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg atggcgcaat ccgggcgcag
    28501 ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg gtttcttcat cagcagtcgc
    28561 cagaatgtag agcggttttt tcagtttcgt gacgtcgaca tcgcgtagcg catcaaggat
    28621 aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg taaagcttgc cgtcatgctc
    28681 cgtcagtgta aacggatcgc gcgtccagcg accgtcatca aatggcaccg tatcggtatg
    28741 ccccgccagc aacaagccgc cagccccctg tccgatactt gccagcatat tgaatttgtt
    28801 gcgagttcct ggcacaggct gcacttccac attgaagcct aaatctttaa accagtccgc
    28861 cagcagagtg attaaatctg cattgctttg atcgagtgcc tcttccgtgg cgcttattga
    28921 aggtgtggca atcagagcac ggtaaatctc gataaatggc ggtaatttgt ttttcattgt
    28981 tgacacacct ctggtcatga tggtatcaat attcatgcag tatttatgaa taaaaataca
    29041 ctaacgttga gcgtaataaa acccaccagc cgtaaggtga atgttttacg tttaacctgg
    29101 caaccagaca taagaaggtg aatagccccg atgttgaata cgctgattgt gggtgccagc
    29161 ggctacgctg gcgcagagct agtgacctat gtaaatcgcc atccgcatat gaacataacc
    29221 gctttgactg tttcagcgca aagcaatgat gcgggaaagt taatctccga tttgcatccg
    29281 cagctaaaag gcatcgttga tctgccgttg cagccgatgt cggatatcag cgagtttagc
    29341 ccaggggtgg acgtagtgtt tctcgccacc gcccatgaag ttagccacga tttagcgccg
    29401 cagtttcttg aagcgggctg cgtggtgttc gacctttccg gcgcgtttcg tgttaacgac
    29461 gccaccttct atgaaaaata ttacggcttt acccatcaat acccggaact gttggaacag
    29521 gcagcctacg gtctggcgga gtggtgcggt aataaattaa aagaagcgaa tttgattgcg
    29581 gtgccgggct gttatccgac ggcggcacag ctggcgctga aaccgttgat tgatgccgat
    29641 cttcttgacc tcaatcagtg gccggtgatc aacgccacca gcggcgtgag cggtgcagga
    29701 cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc tgcaaccgta tggcgtcttt
    29761 acccatcgcc atcaaccaga gatcgccaca cacctcggtg ctgacgttat cttcaccccg
    29821 catctgggca atttcccgcg cggcattctt gaaaccatta cctgccgcct gaaaccgggt
    29881 gtgacccagg cgcaagtggc gcaagcgtta cagcaggcgt atgcccataa accgctggtg
    29941 cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg ttgggctgcc atgttgcgat
    30001 atcgggtttg ccgttcaggg cgagcatctg attattgtgg cgaccgaaga caacttactg
    30061 aaaggcgcgg cggcacaagc ggtacagtgc gccaatattc gtttcggcta tgcggaaacg
    30121 cagtctctta tttaagggtg caatgatgaa tccattaatt atcaaactgg gcggcgtact
    30181 gctggatagt gaagaggcgc tggaacgtct gtttagcgca ctggtgaatt atcgtgagtc
    30241 acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc gtggtggatg agctgatgaa
    30301 agggctgaat ctgccggtga aaaagaaaaa tggcctgcgg gtgacgcctg cggatcagat
    30361 agacattatc accggagcac tggcaggaac ggcaaataaa accctgctgg catgggcgaa
    30421 gaaacatcag attgcggccg taggtttgtt tctcggtgac ggcgacagcg tcaaagtgac
    30481 ccagcttgat gaagagttag gtcatgttgg actggcgcag ccaggttcgc cgaagcttat
    30541 caacaccttg ctggagaacg gctatctgcc ggtggtcagc tccattggcg taacagacga
    30601 agggcaactg atgaacgtca atgccgacca ggcggcgacg gcgctggcgg caacgctggg
    30661 cgcggatctg attttgctct ctgatgtcag cggcattctc gacggcaaag ggcaacgcat
    30721 tgccgaaatg accgccgcga aagcagaaca actgattgag cagggcatta ttactgacgg
    30781 catgatagtg aaagtgaacg cggcgctgga tgctgcgcgc acgttgggcc gtccggtaga
    30841 tatcgcctcc tggcgtcatg cggaacagct tccggcactg tttaacggta tgccgatggg
    30901 tacgcggatt ttagcttaag ttttgttggc cggaggcgca gctttccggc attgaatttc
    30961 aaaataagga aacagagtta tggcactttg gggcgggcgt tttacccagg cagcagatca
    31021 acggttcaaa caattcaacg actcactgcg ctttgattac cgtctggcgg agcaggatat
    31081 tgttggctct gtggcctggt ccaaagccct ggtaacggtc ggcgtgttaa ccgcagaaga
    31141 gcaggcgcaa ctggaagagg cgctgaacgt gttgctggaa gatgttcgcg ccaggccaca
    31201 acaaatcctt gaaagcgacg ccgaagatat ccatagctgg gtggaaggca aactgatcga
    31261 caaagtgggc cagttaggca aaaagctgca taccgggcgt agccgtaatg atcaggtagc
    31321 gacagacctg aaactgtggt gcaaagatac cgttggcgag ttgctgacgg ctaaccggca
    31381 gctgcaatcg gcgctggtgg aaaccgcaca aaacaatcag gacgcggtga tgccaggtta
    31441 cactcacctg caacgcgccc agccggtgac gttcgcgcac tggtgcctgg cctatgttga
    31501 gatgctggcg cgtgatgaaa gccgtttgca ggatgcgctt aagcgtctgg atgtcagccc
    31561 attaggctgt ggcgcgctgg cgggaacggc ctatgaaatc gaccgtgaac agttagcagg
    31621 ctggctgggc tttgcttcgg cgacccgtaa cagtctcgac agcgtttctg accgtgacca
    31681 tgtgttggaa ctgctttctg ctgccgctat cggcatggtg catctgtcgc gttttgctga
    31741 agatctgatt ttctttaaca ccggcgaagc ggggtttgtg gagctttctg accgcgtgac
    31801 ttccggttca tcattaatgc cgcagaagaa aaacccggat gcgctggagc tgattcgcgg
    31861 taaatgcggt cgggtgcagg gggcgttaac cggcatgatg atgacgctga aaggtttgcc
    31921 gctggcttac aacaaagata tgcaggaaga caaagaaggt ctgttcgacg cgctcgatac
    31981 ctggctggac tgcctgcata tggcggcgct ggtgctggac ggcattcagg tgaaacgtcc
    32041 acgttgccag gaagcggctc agcagggtta cgccaacgcc accgaactgg cggattatct
    32101 ggtggcgaaa ggcgtaccgt tccgcgaggc gcaccatatt gttggtgaag cggtggtgga
    32161 agccattcgt cagggcaaac cgctggaaga tctgccgctc gacgagttgc agaaattcag
    32221 cccggtgatt gacgaagatg tctatccgat tctgtcgctg caatcgtgcc tcgacaagcg
    32281 tgcggcaaaa ggcggcgtct caccgcagca ggtggcgcag gcaattgctt ttgcgcaggc
    32341 gcggttgggg taagaacatt tatatgtata aatttgagcc tggcttatcg ccgggctttt
    32401 ttatggcaaa aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc tttagttatt
    32461 cgagttgaga aactctcgaa acgggcagtg acttcaaggg ttaaaagagg tgccgctccg
    32521 tttctgtgag caattatcag tcagaatgct tgatagggat aatcgttcat tgctattcta
    32581 cctatcgcca tgaactatcg tggcaatgga ggatggataa tgaatattcg tgatcttgag
    32641 tacctggtgg cattggccga acaccgccat tttcggcgtg cggcagattc ctgccacgtt
    32701 agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagttggg cgtgatgttg
    32761 ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtagatcag
    32821 gcgcgtaccg tactgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag
    32881 acgatgtccg ggccgctgca cattggtttg attcccacag ttggaccgta cctgctaccg
    32941 catattatcc ctatgctgca ccagaccttt ccaaagctgg aaatgtatct gcatgaagca
    33001 cagacccacc agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg
    33061 ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg
    33121 gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg taccgatggc cgatctggca
    33181 ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc
    33241 tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct ggagacgctg
    33301 cgcaacatgg tggcggcagg tagcgggatc actttactgc ctgcgctggc tgtgccgccg
    33361 gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact
    33421 attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag
    33481 gccatccgcg cacgaatgga tggccatttc gataaagttt taaaacaggc ggtttaaacc
    33541 gtttaacgca gctacccgat aggcttccgc catcgtcggg tagttaaagg tggtgttgac
    33601 gaagtactca atagtgttgc cgccaccttt ctgttccata atcgcctgac cgatatgaat
    33661 aatttcggca gcgcgctcgc caaagcagtg aatacccaga atctcttttg tttcccgatg
    33721 gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt tgtgcgcgtg ccagatgttt
    33781 aaactgggcg cggcccactt catatggcac tttcattgcg gtcagctgct gttcggtttt
    33841 gcccacagag ctgatttccg ggatggtgta aatgccggta gggatatctt caatcagatg
    33901 tgcggtggct tcgcctttta ccagcgcctg cgcggcaatg cgcccctggt cataggccgc
    33961 tgatgccagg ctcggataac caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt
    34021 ctgatacatg ctgttgacct tcagctgtcc gcggctgtca gtttccagcc caatgttctg
    34081 taacgccagc gaatcggtat taccggtgcg accgttggca tagagcaggc agtcagcttt
    34141 cagtttttta cccgacttca aatgcatgat cacaccatcg tcacagcctt cgatcttctc
    34201 gtactcttcg ttgtgacgaa tcactacgcc actgttccag aagtgatagg agagagaatc
    34261 tgacatctct tgatcgagaa atgccagcag gcgatcgcgg gtgttgatca gatccacttt
    34321 tacatccata ccgcggaaga tcgacgcata ttcacagccg atcactccag caccatagat
    34381 aagtacatgg cgcggttcgt ggtgcatgct aagaattgag tcgctgtcgt aaatgcgtgg
    34441 atgggtgaaa tcaacatctg ttggatgata tggacgagag ccgcaggcaa taacaaattt
    34501 ttcagcggtt agtgtttcaa cgctgccgtc cgggcaatcc agcgccaacg tatgctcgtc
    34561 aacaaagcga gcgtttccct gcaatatttc acagtgatta cgttcgtaaa atccctgacg
    34621 catgcgcgtt tgttgattaa tcacgttatc ggcatggtta aggatatcgg caaaagaaga
    34681 gcggagcagt cgggaatggt cgctgtaaag tgggttttga ttgaattcta taatgcggct
    34741 gacggcgtga cggagagctt tcgacgggat ggtgccccag tgggtgcaac cgccgccaac
    34801 attttgataa cgctcgataa ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc
    34861 gccttcgccg ccggggccgg aacctattac tatggcatcg taatcgtagg aatgtggcat
    34921 ggtagggctt acctgttctt atacataaaa gcaacagaat ggtaacattt tatcgcgggt
    34981 aagccaattg atccccgtca tttatctggc tatatcctga gcggcctttg ctttgtctgt
    35041 ttcttacttt tgccctgacg ttttattgga tttttatcga cgatactctc cgtttaagcg
    35101 gcaggtttcc gctgtacgta aaagaaccgg ccaaagaatt gcagtaaata tgttttattg
    35161 cgttaccgtt cattcacaat actggagcaa tccagtatgt tcattctctg gtatagtgcc
    35221 agcagtactt ttggcaagga ttcagacatc gtgatgggcg taagagcgca acaaaaagaa
    35281 aaaacccgcc gttcgctggt ggaagccgca tttagccaat taagtgctga acgcagcttc
    35341 gccagcctga gtttgcgtga agtggcgcgt gaagcgggca ttgctcccac ctctttttat
    35401 cggcatttcc gcgacgtaga cgaactgggt ctgaccatgg ttgatgagag cggtttaatg
    35461 ctacgccaac tcatgcgcca ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgc
    35521 acctcggtct ccacatttat ggagttcatc ggtaataatc ctaacgcctt ccggttatta
    35581 ttgcgggaac gctccggcac ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag
    35641 cacttcattg cggaacttgc ggactatctg gaactcgaaa accatatgcc gcgtgcgttt
    35701 actgaagcgc aagccgaagc aatggtgaca attgtcttca gtgcgggtgc cgaggcgttg
    35761 gacgtcggcg tcgaacaacg tcggcaatta gaagagcgac tggtactgca actgcgaatg
    35821 atttcgaaag gggcttatta ctggtatcgc cgtgaacaag agaaaaccgc aattattccg
    35881 ggaaatgtga aggacgagta atgaaacaag caaatcaaga tagaggtacg ctgctgctgg
    35941 cgttagttgc tggcttatcg attaatggta ctttcgcagc gctgtttagc tccattgtgc
    36001 cattttctgt attcccgatt atttccctgg tgctgacggt ttacagcctg catcaacgtt
    36061 atcttaatcg cactatgccg gtaggcttgc cgggtctggc agctgcctgt tttattctcg
    36121 gcgtactgct gtacagcacg gtagttcgtg cggaatatcc ggatatcggc tctaacttct
    36181 tcccggcagt actctccgtc attatggtgt tctggattgg cgcgaagatg cgtaaccgta
    36241 agcaggaagt tgctgaataa tcggtattat gccggatgct ggcgcatcca gcatgggttt
    36301 tacttcgcgg tcagtaatac gccgcactcc atatggtgcg tgtaggggaa ctgatcaaac
    36361 agagccagac gttcgacctt gtgcgtctgg cttaatgttt ccagattctt gcataacgtt
    36421 tccgggttac aggagatgta caaaatacac ggatacgcct gcaccatttt ctcggtttca
    36481 ctgtccagac cgctgcgcgg tgggtcgaca aaaatggttt cgcactgata actctttaag
    36541 tcgatccctt gcaggcggtt aaactcgcgt acaccattca tcgcctgagt aaattcttct
    36601 gccgccatac gaataatttg tacgttatca atatggttag ctgcgatgtt gtattgcgca
    36661 gcagcaaccg acggcttagc gatttcggtg gctaataccc gatcaaaatt gcgcgccagc
    36721 gctaatgaaa agttaccgtt gccgcagtac agctccagta aatcgccttt tgagcctttg
    36781 gttacgtcca gcgcccattc cagcatctga atattcatcg ccgcgttcgg ctgggtaaag
    36841 ctgttttcta cctgacggta gatcatctct ttccctgcga ccggcagacg ctcgtcgatg
    36901 taatcttgat ccagctcgat tttggttttc gttgcccgac caatcagatg cacattcaga
    36961 ttctgcgcgc gcagtgcatc gcgcagggcc tccgcttcct gacgccactc atcatccagc
    37021 ttcttatggt atagcaggga aaccaccgcc tgattactca gcgtggtgag gtaatcaatc
    37081 tggaacaact tgtggcgcag aacgggatta ttacgcacac ccgcaatcat cgccgtcatc
    37141 aactggttga taagttcact ggcggcgggg aagctatcca cgcggatgcg gcttttggtt
    37201 tgttgatcga aaatgatgtg atacaggtca tcgccatcgt gccagatgcg gaactccgcg
    37261 cgcatccggt aatgactgac cggcgagcga aacacttccg gaaccaggtc agaaaacggt
    37321 gccatcatac tttgcaaacg taccactttt tcggctaact gcgcttcata ctgttctgtt
    37381 ggaaggtgtt cgggggtcat gatgtatcct gaaaaattaa agtacgcggc gattgtaggg
    37441 attgctcatc agatgtccag atcttgatga attcctattt gtgagctacg tctggacagt
    37501 aacttgttac aacctgtagc atccacttgc cggtcctgtg agttaatagg gaatccagtg
    37561 cgaatctgga gctgacgcgc agcggtaagg aaaggtgcga tgattgcgtt atgcggacac
    37621 tgccatccgg tgggaagtca tcatctctta gtatcttaga tacccctcca agcccgaaga
    37681 cctgccggcc aacgtcgcat ctggttctca tcatcgcgta atattgatga aacctgcggc
    37741 atccttcttc tattgtggat gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg
    37801 ttctgtcaca gccttttccg cttgggcaca ggataccagc ccggatactc tcgtcgttac
    37861 tgctaaccgt tttgaacagc cgcgcagcac tgtgcttgca ccaaccaccg ttgtgacccg
    37921 tcaggatatc gaccgctggc agtcgacctc ggtcaatgat gtgctgcgcc gtcttccggg
    37981 cgtcgatatc acccaaaacg gcggttcagg tcagctctca tctattttta ttcgcggtac
    38041 aaatgccagt catgtgttgg tgttaattga tggcgtacgc ctgaatctgg cgggggtgag
    38101 tggttctgcc gaccttagcc agttccctat tgcgcttgtc cagcgtgttg aatatatccg
    38161 tgggccacgc tccgccgttt atggttccga tgcaataggc ggggtggtga atatcatcac
    38221 gacgcgcgat gaacccggaa cggaaatttc agcagggtgg ggaagcaata gttatcagaa
    38281 ctatgatgtc tctacgcagc aacaactggg ggataagaca cgggtaacgc tgttgggcga
    38341 ttatgcccat actcatggtt atgatgttgt tgcctatggt aataccggaa cgcaagcgca
    38401 gacagataac gatggttttt taagtaaaac gctttatggc gcgctggagc ataactttac
    38461 tgatgcctgg agcggctttg tgcgcggcta tggctatgat aaccgtacca attatgacgc
    38521 gtattattct cccggttcac cgttgctcga tacccgtaaa ctctatagcc aaagttggga
    38581 cgccgggctg cgctataacg gcgaactgat taaatcacaa ctcattacca gctatagcca
    38641 tagcaaagat tacaactacg atccccatta tggtcgttat gattcgtcgg cgacgctcga
    38701 tgagatgaag caatacaccg tccagtgggc aaacaatgtc atcgttggtc acggtagtat
    38761 tggtgcgggt gtcgactggc agaaacagac tacgacgccg ggtacaggtt atgttgagaa
    38821 tggatatgat caacgtaata ccggcatcta tctgaccggg ctgcaacaag tcggcgattt
    38881 tacctttgaa ggcgcagcac gcagtgacga taactcacag tttggtcgtc atggaacctg
    38941 gcaaaccagc gccggttggg aattcatcga aggttatcgc ttcattgctt cctacgggac
    39001 atcttataag gcaccaaatc tggggcaact gtatggcttc tacggaaatc cgaatctgga
    39061 cccggagaaa agcaaacagt gggaaggcgc gtttgaaggc ttaaccgctg gggtgaactg
    39121 gcgtatttcc ggatatcgta acgatgtcag tgacttgatc gattatgatg atcacaccct
    39181 gaaatattac aacgaaggga aagcgcggat taagggcgtc gaggcgaccg ccaattttga
    39241 taccggacca ctgacgcata ctgtgagtta tgattatgtc gatgcgcgca atgcgattac
    39301 cgacacgccg ttgttacgcc gtgctaaaca gcaggtgaaa taccagctcg actggcagtt
    39361 gtatgacttc gactggggta ttacttatca gtatttaggc actcgctatg ataaggatta
    39421 ctcatcttat ccttatcaaa ccgttaaaat gggcggtgcg agcttgtggg atcttgcggt
    39481 tgcgtatccg gtcacctctc atctgacagt tcgtggtaaa atagccaacc tgttcgacaa
    39541 agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct tgtctggcag
    39601 ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt
    39661 cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat gctttcgata
    39721 acgtcgcttt tccgtatggc gaaaaaagcg aagagtttat tgttgagcga gtggtggcaa
    39781 ttgtcaccgc agtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct tgcaacaccg
    39841 ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt gttggtgtcg
    39901 tgccggcgat taaacctgct gcacgtctga cggcaaatgg cattgtcgga ttactggcaa
    39961 cccgcggaac tgttaaacgt tcttatactc atgagctgat cgcgcgtttc gctaatgaat
    40021 gccagataga aatgctgggc tcggcagaga tggttgagtt ggctgaagcg aagcttcatg
    40081 gcgaagatgt ttctctggat gcactaaaac gtatcctacg cccgtggtta agaatgaaag
    40141 agccgccaga taccgttgta ttgggttgca cccatttccc tctactacaa gaagaactct
    40201 tacaggtgct gccagaggga acccggctgg tggattctgg cgcagcgatt gctcgcagaa
    40261 cggcctggct gttagaacat gaagctccgg atgcaaaatc tgccgatgcg aatattgcct
    40321 tttgtatggc aatgacgcca gaagctgaac aattattgcc cgttttacag cgttacggct
    40381 tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatg
//
LOCUS       DAZUFB010000035        39232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZUFB010000035 DAZUFB010000000
VERSION     DAZUFB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.035"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(144..335)
                     /gene="yfgG"
                     /locus_tag="ACHY3M_002057"
     CDS             complement(144..335)
                     /gene="yfgG"
                     /locus_tag="ACHY3M_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFS4316975.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            353..505
                     /locus_tag="ACHY3M_002058"
     CDS             353..505
                     /locus_tag="ACHY3M_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4316976.1"
                     /translation="MPHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            687..2930
                     /gene="pdeF"
                     /locus_tag="ACHY3M_002059"
     CDS             687..2930
                     /gene="pdeF"
                     /locus_tag="ACHY3M_002059"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFS4316977.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2969..4510)
                     /gene="ppx"
                     /locus_tag="ACHY3M_002060"
     CDS             complement(2969..4510)
                     /gene="ppx"
                     /locus_tag="ACHY3M_002060"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFS4316978.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4515..6581)
                     /gene="ppk1"
                     /locus_tag="ACHY3M_002061"
     CDS             complement(4515..6581)
                     /gene="ppk1"
                     /locus_tag="ACHY3M_002061"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFS4316979.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6752..7390)
                     /gene="purN"
                     /locus_tag="ACHY3M_002062"
     CDS             complement(6752..7390)
                     /gene="purN"
                     /locus_tag="ACHY3M_002062"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4316980.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7390..8427)
                     /gene="purM"
                     /locus_tag="ACHY3M_002063"
     CDS             complement(7390..8427)
                     /gene="purM"
                     /locus_tag="ACHY3M_002063"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4316981.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8752..9378
                     /gene="upp"
                     /locus_tag="ACHY3M_002064"
     CDS             8752..9378
                     /gene="upp"
                     /locus_tag="ACHY3M_002064"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4316982.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9464..10753
                     /gene="uraA"
                     /locus_tag="ACHY3M_002065"
     CDS             9464..10753
                     /gene="uraA"
                     /locus_tag="ACHY3M_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFS4316983.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10848..11549
                     /gene="hda"
                     /locus_tag="ACHY3M_002066"
     CDS             10848..11549
                     /gene="hda"
                     /locus_tag="ACHY3M_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFS4316984.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11687..12046)
                     /gene="arsC"
                     /locus_tag="ACHY3M_002067"
     CDS             complement(11687..12046)
                     /gene="arsC"
                     /locus_tag="ACHY3M_002067"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFS4316985.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12067..13530)
                     /gene="bepA"
                     /locus_tag="ACHY3M_002068"
     CDS             complement(12067..13530)
                     /gene="bepA"
                     /locus_tag="ACHY3M_002068"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFS4316986.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13743..14804
                     /gene="yfgO"
                     /locus_tag="ACHY3M_002069"
     CDS             13743..14804
                     /gene="yfgO"
                     /locus_tag="ACHY3M_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4316987.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14842..15690)
                     /gene="focB"
                     /locus_tag="ACHY3M_002070"
     CDS             complement(14842..15690)
                     /gene="focB"
                     /locus_tag="ACHY3M_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFS4316988.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15712..17724)
                     /gene="hyfR"
                     /locus_tag="ACHY3M_002071"
     CDS             complement(15712..17724)
                     /gene="hyfR"
                     /locus_tag="ACHY3M_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFS4316989.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17754..18167)
                     /gene="hyfJ"
                     /locus_tag="ACHY3M_002072"
     CDS             complement(17754..18167)
                     /gene="hyfJ"
                     /locus_tag="ACHY3M_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFS4316990.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18160..18917)
                     /gene="hyfI"
                     /locus_tag="ACHY3M_002073"
                     /pseudo
     CDS             complement(18160..18917)
                     /gene="hyfI"
                     /locus_tag="ACHY3M_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18914..19459)
                     /gene="hyfH"
                     /locus_tag="ACHY3M_002074"
     CDS             complement(18914..19459)
                     /gene="hyfH"
                     /locus_tag="ACHY3M_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFS4316991.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19469..21136)
                     /gene="hyfG"
                     /locus_tag="ACHY3M_002075"
     CDS             complement(19469..21136)
                     /gene="hyfG"
                     /locus_tag="ACHY3M_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFS4316992.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21126..22706)
                     /gene="hyfF"
                     /locus_tag="ACHY3M_002076"
     CDS             complement(21126..22706)
                     /gene="hyfF"
                     /locus_tag="ACHY3M_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFS4316993.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22711..23361)
                     /gene="hyfE"
                     /locus_tag="ACHY3M_002077"
     CDS             complement(22711..23361)
                     /gene="hyfE"
                     /locus_tag="ACHY3M_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFS4316994.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23373..24812)
                     /gene="hyfD"
                     /locus_tag="ACHY3M_002078"
     CDS             complement(23373..24812)
                     /gene="hyfD"
                     /locus_tag="ACHY3M_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFS4316995.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24829..25776)
                     /gene="hyfC"
                     /locus_tag="ACHY3M_002079"
     CDS             complement(24829..25776)
                     /gene="hyfC"
                     /locus_tag="ACHY3M_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFS4316996.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25787..27805)
                     /gene="hyfB"
                     /locus_tag="ACHY3M_002080"
     CDS             complement(25787..27805)
                     /gene="hyfB"
                     /locus_tag="ACHY3M_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFS4316997.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27805..28422)
                     /gene="hyfA"
                     /locus_tag="ACHY3M_002081"
     CDS             complement(27805..28422)
                     /gene="hyfA"
                     /locus_tag="ACHY3M_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFS4316998.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCLVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28675..29145)
                     /gene="bcp"
                     /locus_tag="ACHY3M_002082"
     CDS             complement(28675..29145)
                     /gene="bcp"
                     /locus_tag="ACHY3M_002082"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFS4316999.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29145..29729)
                     /gene="gcvR"
                     /locus_tag="ACHY3M_002083"
     CDS             complement(29145..29729)
                     /gene="gcvR"
                     /locus_tag="ACHY3M_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFS4317000.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPRPRPPMPASVWV
                     QVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAA
                     NIEQAFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29875..30753
                     /gene="dapA"
                     /locus_tag="ACHY3M_002084"
     CDS             29875..30753
                     /gene="dapA"
                     /locus_tag="ACHY3M_002084"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4317001.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30770..31804
                     /gene="bamC"
                     /locus_tag="ACHY3M_002085"
     CDS             30770..31804
                     /gene="bamC"
                     /locus_tag="ACHY3M_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFS4317002.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32017..32730
                     /gene="purC"
                     /locus_tag="ACHY3M_002086"
     CDS             32017..32730
                     /gene="purC"
                     /locus_tag="ACHY3M_002086"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4317003.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32884..33111
                     /gene="vapB"
                     /locus_tag="ACHY3M_002087"
     CDS             32884..33111
                     /gene="vapB"
                     /locus_tag="ACHY3M_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFS4317004.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33111..33509
                     /locus_tag="ACHY3M_002088"
     CDS             33111..33509
                     /locus_tag="ACHY3M_002088"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFS4317005.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33656..34519
                     /gene="ypfJ"
                     /locus_tag="ACHY3M_002089"
     CDS             33656..34519
                     /gene="ypfJ"
                     /locus_tag="ACHY3M_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFS4317006.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34534..36549
                     /gene="tmcA"
                     /locus_tag="ACHY3M_002090"
     CDS             34534..36549
                     /gene="tmcA"
                     /locus_tag="ACHY3M_002090"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFS4317007.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36623..37321
                     /gene="ypfH"
                     /locus_tag="ACHY3M_002091"
     CDS             36623..37321
                     /gene="ypfH"
                     /locus_tag="ACHY3M_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4317008.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37431..37631)
                     /gene="ypfN"
                     /locus_tag="ACHY3M_002092"
     CDS             complement(37431..37631)
                     /gene="ypfN"
                     /locus_tag="ACHY3M_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFS4317009.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37659..38786)
                     /gene="dapE"
                     /locus_tag="ACHY3M_002093"
     CDS             complement(37659..38786)
                     /gene="dapE"
                     /locus_tag="ACHY3M_002093"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFS4317010.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38790..39146)
                     /gene="yffB"
                     /locus_tag="ACHY3M_002094"
     CDS             complement(38790..39146)
                     /gene="yffB"
                     /locus_tag="ACHY3M_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFS4317011.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9604 a        10043 c        10305 g         9280 t
ORIGIN      
        1 gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga tgaaagaata
       61 tctgccgctt tgataattcc aaagagcaaa agcatccgac agcgaagtgc cgttaggttc
      121 tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga gagtgtggat
      181 tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga gtagataaaa
      241 cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg actgttaaat
      301 cggtgtcgtt ttcgcatact ggttgcctga ctcacaaaag gttccttgaa gtatgcccca
      361 cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt cttcgttatg
      421 taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa ttatttacaa
      481 tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa tcagggttat
      541 tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat gctctctata
      601 gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca cctccttcag
      661 gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata aaaatacgtg
      721 ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt ttcgcccaca
      781 ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg cctttggcac
      841 tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc accttaggga
      901 tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta acggctaatt
      961 ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc cggcgtaaca
     1021 acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag attgtgtttc
     1081 ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta ctggcgagca
     1141 gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat tatcaggcct
     1201 tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg gtagtgcgaa
     1261 atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat gccaaagtca
     1321 ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg ctgttatgca
     1381 tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca ttattgctgc
     1441 ccctgatgat gtggggagcg atgcgctatg gttataagct gatttcgctg ctctgggcgg
     1501 tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat cctggctata
     1561 ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt gtcaattata
     1621 tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg cttgcgtatg
     1681 tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg cgtgatgccc
     1741 cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt aagaactatg
     1801 gcatcatgct gcggattcaa tacaagcaaa aactttctca ctggctgtca cccttgctgg
     1861 aaccgggtga agatgtttat cagctttcgg gtaacgatct cgcgctgcgg ctgaatacag
     1921 aatcgcacca ggagcgcatt accgcactgg atagccatct caagcaattt cgtttctttt
     1981 gggatggcat gccgatgcaa ccgcagattg gcgtcagtta ctgctatgtg cgctcgccag
     2041 tgaatcatat ctacctgctg ctgggagagc taaatacggt cgccgaactt tccatcgtga
     2101 ccaacgcccc ggaaaatatg cagcgtcgcg gggcaatgta tttgcaacgc gaattgaaag
     2161 ataaagtcgc gatgatgaat cgactacagc aggcgctgga acacaaccat tttttcctga
     2221 tggcccagcc gattaccggt atgcgtggtg atgtttacca tgaaattctt ctgcgcatga
     2281 aaggtgagaa tgatgaactg atcggccccg atagcttctt gccggtcgcg cacgaatttg
     2341 gtttatcgtc gagtatcgac atgtgggtca ttgagcatac gctgcaattt atggctgaaa
     2401 acagagcgaa gatgcccgct caccgttttg ctattaatct gtctccaacc tcggtatgtc
     2461 aggctcgttt tcctgttgaa gtcagtcagt tgctggataa atatcagatt gaagcgtggc
     2521 aacttatttt tgaagtcacc gaaagtaatg ctctgaccaa tgttaagcag gcgcaaatca
     2581 ccttgcagca tcttcaggaa ttaggctgcc agattgcgat tgatgatttc ggcaccggct
     2641 acgccagcta tgcgcggctt aaaaatgtga atgccgatct gcttaaaatt gacggcagtt
     2701 ttatccgcaa tattgtgtca aatagtctgg attatcagat agtggcatcg atttgccacc
     2761 tggcgcgaat gaagaaaatg cgggtagtgg cagagtacgt tgaaaacgaa gagatccgcg
     2821 aggcggtgct ctctttgggg atcgattata tgcagggtta tcttattggt aagccgcaac
     2881 cgttaattga tacgctgaat gaaatcgaac ccattcgcga aagtgcctga ataatgcggg
     2941 ccgacatttc tcgtcggccc gcaaagcatt aagcggcgat ttcaggtgta ctttcttctt
     3001 caattttcaa ccgccagcca gccacgcctt cccagtattc ttgctccttt tccagatcaa
     3061 gcagtaccag cgcattctga ctaaaccagt catgcgggaa acgcagtgtc cagtgactgt
     3121 catcagtaat cagtgtcaat gttggcggtg tggtggttgc ctgacgttga ttgtttagta
     3181 atacgccaag gcgcaatagc tgtatcagtg gcaggaactg tttcttctta aacaaggtaa
     3241 agcgcggcag atcgtcgagc ttaatcgctt tacggtgata gcgcaccagt gtcgccatca
     3301 tcagctgctg ttcctgatta aaacccggca agtcactgtt ttgcagaata taagcggagt
     3361 ggcgatgcaa accgctgtgg ttgatattca acccgacctc atgcagcatg gcggcccatc
     3421 gcagtagcgc ctccagttgc ggatgcgcca gcttcggttg ctgttcccgc cactgttcgt
     3481 acatttgcat agtggtatcc agcactcgtc gggcctgttc gctgtcgatg tgatactggt
     3541 tggcgaggct gctggcggtg cgactacgca catcctgatg acggaaacgt ccttccattt
     3601 catacagtac gccttcgcgt aacgccccgt cagaaaggcg cagttcacgg atggctaaag
     3661 catcaaacac accacataaa atcgccagtc caggaacgaa gactgttttc cgctcttcgg
     3721 aaagacccgg taaactcagc gatgcgaaat tacggtgacg taaaacttct tttaccagtt
     3781 tttccagacg ttccggggta attatcccgt ctttctcgcc catttccatt aatacttcat
     3841 gggcggcttt tatggtaccg gaagcgccca ttgccacgtt ccagccctga atacggaatt
     3901 gccaggttaa agtttccagt ttttgtgctg ccgccatgcg agcgcgctga aaattctctt
     3961 tattgatgac cccgccaggg aaataaagct gggcaaagct gacacaaccc atccggcggc
     4021 tttcaacgag aataggttcg aaattttcac caatcaccag ttccgtagat ccgccgccaa
     4081 tatcaataac cagtttgcga cctttttccg gttgggtatg ttccacgccc ataaaaatca
     4141 gacgggcttc ttcattaccg gaaataattt caatcgggta gggaatgacc ttttccgcgc
     4201 gtttcagaaa gtcagtggcg ttcagcgcct gacgcagtgt atgggtgcca actatacaga
     4261 cgctggcagg agaaaaccct tgtagccgtt cggcaaacag cgacagacag ttcaaaccgc
     4321 gcgtcattgc ctcttcactc aacatattat ctggccccag gccgtccgcc agatgcaccc
     4381 gctgtttcag gcggccaata atctgcatgg caccatctac cacacgggct atgaccatgt
     4441 gaaaactgtt tgaaccaaga tcgaccgcag caaactcctg cggacgaggg gatttatcgt
     4501 gtattggcat agggttattc aggttgttcg agtgatttga tgtagtcata aatcgccaac
     4561 tgcgcccgta ctttgcggcg attgccgcgg ggaacgtagc gattactgag ttctttatcg
     4621 atataacgtg ctttgaccgt atcgctgaac aatatgtcga tgatgtccag cacccgctgc
     4681 ttcaggcgcg gatcgagcag cggtgtcgcc acttcaatac gataatcaat attgcgcgtc
     4741 atccagtcgg cggaagaaag gtagaccttt ttatcgccgc cattttcaaa aatataaacc
     4801 cggtcatgtt caaggtaacg gtcaacaata ctgatggcac gaatgttgtc gctaatgcct
     4861 tccagattgg ggatcagcga acacattccg cgaaccagca gattaaccgg tacgccggag
     4921 ctggaggccg catacagacg atcaaccagg cctttatcga caaggttatt tagcttcagg
     4981 gtgataccac tgggcagccc ttgctgcacg ttggcgattt cgcggtctac catttcatat
     5041 aacagacggc gagagttttg cggtgacacc attaaataat caaatgtcac tgggcggtat
     5101 gggttttcaa taaagttaaa tacccgccgt acttcgttgg tgatacgcgc atctgcggtc
     5161 agcaacgaat agtcagtata aagacgcgcg gttttttcgt taaagttccc ggtcccgatg
     5221 tgtgcgtaac ggaccacttc accgttttct ttacgtgaaa tcaggaacag tttggcgtga
     5281 attttcagcc ccggcgcaga gaagataacg tgcacgcctg cttcggtcag gcgcttcgcc
     5341 cagtgaatgt tggcttcttc gtcgaaacgc gcctgtaact caaccaccac agtgactttc
     5401 ttaccgttat gtgcggcgtg gatcatcgag tcgatgatgc gtgaatcttt cgccacgcgg
     5461 taaatgttaa ttttaatcgc cagtacgctc gggtcgaacg aagcctgacg cagcagttcc
     5521 agcacatgct caaaggtgtg ataaggataa tagagcaaca catcgcgttc gcgaatggca
     5581 tcaaaaccat tgcggaactg ggctttatca aaccaaatgt ggcgtaaacg cggcagtggt
     5641 ttgttcacca gattggcttt gccgacattg gggaaattaa taaagtcttt aaaattatga
     5701 taacgaccgc cggggacgat ggagtcgtag cgggaaatag tcagtttttc gcgtaacact
     5761 tcaaccagcg cattgggcat atcgcgctga taaacaaaac gcaccggctc agcagttaaa
     5821 cgctgcttga gactggaaga catcaactcc atcaggctgg cttccatctc atgcactaaa
     5881 tcgtattcgg catcgcgtgt catcttcatt gaataggcat tcagcgcgtc ataatcaaag
     5941 aagcctttga aaatatcatc aaggcagtaa cgcagaatgt tatccagaag aatcatcggc
     6001 ttgcgtcgac gcggcgcttc tggcggtaaa ttcacaaagc gcggcacttt atctgatggg
     6061 atctccagca gcgcgtaacg gatggtatca ccacggataa tttccaccgc cagataggtg
     6121 taatcatcct tcaggaactg cactaagtca gtgtcaggat tgattaaaat cggcgtaatg
     6181 tgctgacgca gatactgctt aaaataatga cgaagccagt tttgttgatt gacggagagc
     6241 tggcgttcat taatcaggaa gatctggttg cgcgccatct ccagcaatag ctcgttgtag
     6301 aggccgtcga attcctgatc ggctttcagc acccgggact gaattttgcc cagtaaatgg
     6361 cgggaatgag agttggagcc ttgttcttcg ctaataatga tgcgtcgctt cagttcagcg
     6421 aagcggactt tatagaactc atcaaggtta ttggaataga tccccaggaa acgcatcctt
     6481 tcaatcagcg ggttagattt gtccgccgct tcctgaagca cgcgttcatt gaacgataac
     6541 caactgagct ctttttcgat gtatagcttt tcctgaccca ttaccacttt tactccgttt
     6601 tattcacggg acactgcctg gatattatgg cgagcaatat tggcagatgt ccaactatgc
     6661 cagaaaaggg ggaaaaaaac tgctggagct tgcgatttac aggtgaattg aaaaacgcca
     6721 gcggcagagc tggcgcttta actacggggc attactcgtc ggcagcgtag ccctgcggcg
     6781 gcagacgttg accatccagc cacgcggcgt tttcgtgcat tttcagacga ccatcggcaa
     6841 accagctaat caccagtgga taaatagcgt gttcctgggt ttgcacgcgg gcggtgatgt
     6901 catcttccgt atcaccagca aataccggga ctttcgcctg taaaataacc gggccaccgt
     6961 ccagttcatc ggtgacgaaa tgcaccgatg taccgtgctc ttcatcacca ttttccagcg
     7021 cttgacgatg ggtgtgtaat ccgggatatt tcggcagcag agaagggtga atgttcagca
     7081 aacgcccggc atagtgggag acaaacgccg ggctgagaat gcgcataaaa ccagccagca
     7141 cgaccacatc gggtgcgtac atgtcgattt catgaatcaa ctcccggtca taggcttcac
     7201 gactgtcaaa cgcgctggcg atgagcgtat gcgttgcaat acccgcctgg cgggcgcgtt
     7261 caaggccgaa cgcgtcggcc ttattgctga aaactgcccg tacggtgcct ttaattttgt
     7321 tggttttaca ggcgtcaata attgcctgta aattacttcc gttaccggaa ataagcacca
     7381 caatattcat tattcgataa ccacgcgttg ttcggaatca gaggctttga tgataccgat
     7441 tttccacgcg ttttcaccgt tggcattgag cagggcgagg gctttgtcca cttccggagc
     7501 aggcagggca ataatcatcc cgacgccgca gttgaaggtg cgatacattt catggcgctc
     7561 aacgttacct gccgtttgca gccagttgaa cacttccggc cactgccagg aagattcatc
     7621 aatcactgcc tgagtattat ctggcaatac gcgtggaatg ttttcccaga agccgccgcc
     7681 ggtcaggtgc gcaatggcat gcacatcgac cttttcaatc aactccagca ctgacttcac
     7741 gtaaatgcgg gtcggtgcca gcagataatc ggctaatggc ttaccatcaa gttgggtggt
     7801 ttgcggatca caaccgctga cttcaagaat tttgcgcacc agcgaatagc cgttcgagtg
     7861 cggaccgctg gaaccgagtg caatcagcac atcgccgtcg ctgactttag agccgtcgat
     7921 gatttctgat ttttctacca cgccaacgca gaaacccgcg acatcgtaat cctcaccgtg
     7981 atacatcccc ggcatttctg ccgtttcgcc acccaccagt gaacagcccg attgcagaca
     8041 tccttccgcg ataccgctga tcaccgctga agcggtatca acatccagtt ttccggttgc
     8101 gtaatagtcg aggaaaaaca gcggctctgc accttgcacc accaggtcat taacgcacat
     8161 ggcgaccaga tcaataccaa tggtgtcgtg acgttttaag tccattgcca gacgcagctt
     8221 ggtacctacg ccgtcagtgc cggaaaccag cacgggttca cgatattttt gcggcaatgc
     8281 acacagcgca ccgaagccgc ccagaccgcc catcacttcc ggacgacgcg ttttcttcac
     8341 tacgcctttg attcttccaa ccagagcatt acccgcgtca atatcaacac cggcatcttt
     8401 gtagctaaga gaggttttat cggtcactgc ttgggtcccc acgcgttact tgcggtagaa
     8461 aaataaaatt cggcgcaatt ctaacaggga aagcaaacgt ttgcgagact gctttacaca
     8521 acctttttgc acgtcttttg cccaggcgcg cggcgaaaga agacttgtac cagggtaaag
     8581 gttagttttc ggatggaata atcttctttc ataaccattt gaatataaaa taactttatc
     8641 tcaagccgtt atcattttga ctaaagtcaa cgaaaagaat attgccgcct tgaagaaagg
     8701 aggtataatc cgtcgatttt ttttgtggct gcccctcaaa ggagaaagag tatgaagatc
     8761 gtggaagtca aacacccact cgtcaaacac aagctgggac tgatgcgtga gcaagatatc
     8821 agcaccaagc gctttcgcga actcgcttcc gaagtgggta gcctgctgac ttacgaagcg
     8881 accgccgacc tcgaaacgga aaaagtaact atcgaaggct ggaacggccc ggtagaaatc
     8941 gaccagatca aaggtaagaa aattaccgtt gtgccaattc tgcgtgccgg tctggggatg
     9001 atggacggtg tgctggaaaa tgttccgagc gcgcgcatca gcgttgtcgg tatgtaccgt
     9061 aatgaagaaa cgctggagcc ggtaccgtac ttccagaaac tggtttctaa catcgatgag
     9121 cgtatggcgc tgatcgttga cccaatgctg gcaaccggtg gttccgttat cgcgaccatc
     9181 gacctgctga aaaaagcggg ctgcagcagc atcaaagttc tggtgctggt agctgcgcca
     9241 gaaggtatcg ctgcgctgga aaaagcgcac ccggacgtcg aactgtatac cgcatcgatt
     9301 gatcagggac tgaacgagca cggatacatt attccgggcc tcggcgatgc cggtgacaaa
     9361 atctttggta cgaaataaag aataaaaata attaaagccg actttaagag tcggcttttt
     9421 tttgagtaaa gcgcctataa cacataatac agaggataat actatgacgc gccgtgctat
     9481 cggggtgagt gaaagaccgc cacttttaca gacaatcccg cttagtttgc aacatttgtt
     9541 cgccatgttt ggtgcaaccg tcctggtgcc cgtcttattt catattaacc cggcgactgt
     9601 actgttattt aacggtattg gaacgctgct gtatctcttc atctgtaaag ggaaaattcc
     9661 ggcttatctt ggttccagct ttgcctttat ttcaccggta ttgttactgt tgccgttagg
     9721 gtatgaagtc gcgctgggcg gctttattat gtgcggcgtg ctgttctgcc tggtttcttt
     9781 tatcgtgaag aaagcgggga ccggctggct ggacgtgctg tttccacctg cggcaatggg
     9841 cgcaatcgtt gccgtcatcg gtctggagct ggcgggcgta gctgccggta tggcgggttt
     9901 actcccggct gaagggcaaa cgccagactc caaaaccatc atcatctcta ttaccaccct
     9961 ggcggtcacg gttttaggtt ccgtgctgtt tcgtggtttc ctggcaatta tcccgatttt
    10021 aattggcgtg ctggtggggt acgcgctctc tttcgcaatg ggaattgtcg ataccacgcc
    10081 gattattaat gctcactggt ttgcgctgcc aaccctctat acgccgcgct tcgagtggtt
    10141 tgccattctg actattctgc cagcggcgtt agtggttatt gccgaacacg tagggcacct
    10201 ggtagtaacg gctaatatcg tcaaaaaaga tctgctgcgc gatccaggtc tgcaccgttc
    10261 gatgtttgct aatggcttgt cgaccgtgat ttccggcttc tttggctcta cgccaaatac
    10321 cacttacgga gaaaacattg gcgtgatggc gatcacccgt gtttacagta cctgggttat
    10381 cggcggggcg gcgattttcg ctatcctgct ttcctgcgtc ggtaaactgg ctgccgctat
    10441 ccagatgatc ccattgccgg tgatgggcgg cgtttcgctg ctgctttatg gtgtcatcgg
    10501 tgcttccggt attcgtgttt tgatcgaatc gaaagtggat tacaacaaag cacagaacct
    10561 gatcctgact tccgtgattt tgatcatcgg cgtcagtggc gcgaaggtaa acatcggcgc
    10621 ggcggagtta aaaggtatgg cgctggcgac catcgtcggt atcggcttaa gtttgatctt
    10681 caaactgatc agtgtgctgc gccctgaaga agtggtgctg gacgcagaag acgcggatat
    10741 aacagacaaa taatgtaccg accgggcagt gttgctgccc ggttcaaaca tcatgggatt
    10801 ctgtggtaaa cttctcgcga ttttgtgaaa tcctggttga ggtatctctg aacacaccgg
    10861 cacagctctc tttgccactt tatcttcctg acgacgaaac ctttgcaagt ttctggccgg
    10921 gggataactc ctctttactg gctgcgctgc aaaacgtgct gcgtcaggaa catagcggtt
    10981 acatctatct ctgggcacgc gaaggcgcgg ggcgcagcca tctgctgcac gcggcttgcg
    11041 cggaattgtc gcagcgtggc gatgcggtgg gctatgtccc gctggataaa cgcacctggt
    11101 ttgttccgga agtgctcgac ggtatggagc atttgtcgct ggtctgtatc gacaacattg
    11161 agtgtattgc aggcgatgag ttgtgggaga tggcgatttt cgatctctac aatcgaattc
    11221 tggaatcggg caaaacacga ttgttgatca ccggcgatcg tccaccgcgg cagttgaatc
    11281 tgggattacc ggatctcgcg tcgcgactcg actgggggca gatctacaaa ttgcagccac
    11341 tttctgatga agataagttg caggcgctac agttacgcgc gcgtttgcgt ggttttgaac
    11401 tgccggaaga tgtggggcgt ttcttgctga agcggctcga cagagaaatg cgcacgctat
    11461 ttatgacgtt ggatcagttg gatcgtgcgt cgattaccgc gcaacgtaag ctgaccattc
    11521 cgtttgtgaa agaaattctg aagttgtaga taaggtgttt attgtcggat gcggcgcgaa
    11581 cgccttatcc gaactacgcg aagttaatcc atagtattcg taggccggat aagacgtggc
    11641 aagcgtcgca tccggcaatg tgcttcaact agcgatggag cacatcttaa cccacgattt
    11701 ccagtacctg ctccggtgga cgaccaatcc gcgctttgcc attcgcgacc acaatcgggc
    11761 gctccattag cttcggatta tccaccattg cctgaatcag cgcctcttcg ctaagcgaac
    11821 tgtcagccag gttcagttct ttataaagat cctctttctg gcgcatcaat tcacgggcgc
    11881 tattcatccc cagcattttc agcaaatcgc gcagcgttgc cgcatcggcg ggtgtctcaa
    11941 ggtagagtac cacttctggc tccacgccgt tttctttcag caggttcagc gtttcccggc
    12001 tctttgaaca gcgtgggtta tggtagattt ttacctgttt ggtcattgtt cttcctttaa
    12061 tgcgaattac atcttggtat aaggcttaaa gcgttcctgc agctggcgca actggtcgat
    12121 gcgcgcatcg taacgcgctt gttgcaggct gcctaatttc acctgcgaac tggcgctact
    12181 caacagcgaa atggcctgat cgagtcgtcc ggcgagcgca taaccttctg ctcgcgcagc
    12241 cagctcctga tcgcggttat ttagcgcggc ttccgcctgt gccagtaaat cccagccgtt
    12301 gctgtcatct ttattattaa aggtgtagcg attcagaata ttggccgctt cttgtggttg
    12361 accgccttgt agataagcgt tcgccaggtt gagctgcaac accggattgg tgcgcaaatc
    12421 acgggcattt ttcaggcgat tgattgcctc attggctttg ttttgcccaa gatcgatatc
    12481 ggtagccaga tcgagatacc atgcgttgcc aggttctgcc gccagtaacg gttgcagcgt
    12541 tttacgcgcc tcgtcgtatt tattggcttc catcgcctgt aaagcacgac catattgcgc
    12601 cgctcgttgc tgacgaacgt ttcctttcgc ccattcatcc agcaaatcac tggtgagctg
    12661 gttacgtccg gaattataca tccccagtgt gcgcgctttc gccagataga aatcttccga
    12721 cgactgcacc accatcggtc gcatctgatt agcacggttg cgggcatctg ccagacgact
    12781 ttccggcaac gggtgagtca ataaaatttc cggcgggcgc gaggagtaac gcgcctgatc
    12841 gagtaatttt tcgaggaagg ttggcatcgc ctgcggatcg aatcccgagc gttgcagcac
    12901 ctgaatacca atgcggtccg cttcctgttc attttgctgg gtgaaactga tcattccctg
    12961 acgtgttccc gccagtgtac cggtcagcgc cgccatcccc gcctgcggac tggccatcgc
    13021 cagtaaaata gaacctaacg cgccgaccca ggtcagcggc gcgctgcgtt gttgatcttc
    13081 catcgctcgc gccaggtgac gttgggtgac gtgggagatt tcgtgcgcca taactgaagc
    13141 cagttgactt tcgttatcgg aataacggaa cagggcagag tgcagcacca cgttgccgcc
    13201 aaagaaagca aaggcgttaa tttcgtcgtt gttgatcaga aaaaaatgaa acggtgtctt
    13261 aaccgaattg gcatgcgaaa ccagacgcat ccccagcgaa ttaatatatt gcgttaacag
    13321 cgggtcatta attaacggcg cgctgccgcg tagctggcgg acataatagt cgcccatctg
    13381 catttcctga ccaatggaaa gcgtgcttcc tgcggaggtt cccatatccg gcaaggtgtc
    13441 tgcgctgtcg gcaaacgccg gggctacctg accaatggtc atagcagcaa tgagggttgc
    13501 aaccaggttt tttttcaact gcctgaacat aacctctatc ctgtatttct gaagatagtc
    13561 atttgaccga tactgttaaa tatcgttccc accgcgccgc ctttgagtgt aacggcgaag
    13621 attagcccac tcaaatgact tttcagtttc ctgaatccgt cataaaaagc gaagtctttt
    13681 ttgtgacatt tcgatacaat tgcggatcgc aatcccgctc ttgaggttca gggaaggttt
    13741 ttatgctcga aatgttgatg caatggtatc gccgccgttt tagcgacccg gaagcgattg
    13801 ccttgctggt tattttagtt gccggatttg gcattatctt tttctttagt ggcctgcttg
    13861 ctccgttgct ggtggctatt gtgctggcct atttgctgga atggccaacc gtgcgcctgc
    13921 aatctattgg ctgctcccgc cgctgggcga cgtcgattgt attggtggtt tttgtcggta
    13981 tattgctact gatggcgttc gtggtactgc ctatcgcctg gcaacagggc atctacttaa
    14041 tccgcgatat gccggggatg ctcaataagc tttctgactt tgccgccacg ttgccgcgcc
    14101 gctatccggc gttaatggac gcgggcatta ttgatgcaat ggccgaaaat atgcgcagtc
    14161 ggatgctgac catgggcgat tcggtggtga aaatttccct cgcctcgctg gtcggtttgc
    14221 tgaccattgc cgtctatctg gtgctggtgc cattgatggt cttcttcctg ctgaaagaca
    14281 aagagcagat gctgaacgcc gttcgtcggg tgctgccgcg taaccgtggg ctggcaggac
    14341 aggtgtggaa ggagatgaat caacaaatca ccaactatat ccgcggcaaa gtgctggaga
    14401 tgatcgtggt agggatcgcc acctggctgg ggttcttgct cttcgggctg aactattcgc
    14461 tgctgctggc ggtgctggtc ggcttctcgg ttcttattcc gtacattggc gcatttgtgg
    14521 tgaccattcc ggtggttggc gtggcgctat tccagtttgg cgcaggcacg gaattctgga
    14581 gctgcttcgc ggtgtatctg attattcagg cgctggacgg caacctgcta gtaccggtgt
    14641 tgttctccga agcggttaac ctgcatccgc tggtgattat tttatcggtg gtgatcttcg
    14701 gtggtttgtg gggattctgg ggcgtattct tcgccattcc attggcgacg ctgatcaaag
    14761 ccgtgatcca cgcctggccc gatgggcaaa tcgcgcaaga ataatcttca gcccgacgaa
    14821 ctgtttcgcc gggctgattt ttcaggattc ctgacgtaaa taaatagccc gataacacat
    14881 actcaccagc actgcaccgc cgataatatt acccagcatc accgggagca gattggcggt
    14941 aataaaatgg ctgaccgtca gtgccggaaa attgtctgcg ctactgtgcg ccagttgcca
    15001 gaaggaggta ggggcgaaat ggcgaatggc aatggcgaac ggaatcacaa acaaattggc
    15061 gatacagtgc tcaaagccac tggcgacaaa cagggtgatg ggcaaaatca tggcgacgat
    15121 tttgtcgcat aacgaacgcc cgcaataact catccacagc gccaggcaaa ccatcagatt
    15181 gcacataatg ccgaggctga cagattcagt aaatgtatga tgcattttgc cctcggcgca
    15241 gtgtaaaacc gcgactcccc acatcgcgtt ttcactcatc accagcccgg aaaaccagat
    15301 taacaaactg aaacaggcaa tacctgccag attaccgcag gccaccagaa gtgcgttaat
    15361 cagccaagtt cgccaactaa taacgcccct actttttgcc attaccgtca ttaccgacga
    15421 ggtgaacagg ttggtgccgc aaaccgccag caaaataaag ccaagtgtaa agcaaaggcc
    15481 gcccaccaga tgggttaatg cctgtgaaga cggggcatcg gcgataactg aaaggtaaaa
    15541 agtaaatccg atcgccataa acacgccagc ggacatcgcc attaaaaaga ccgacacttt
    15601 actgcgggca attttatggg cggcaatccg ttcagcgatt gccgcttttc tggcgctcaa
    15661 ctgcaagtcg aaagagagtt tgtttctcat gcatactcct gcaaaagcag attacaacac
    15721 ctcgcgaacc gagattccca gacgctgcat tcgtgacagc agcgtggtgc gcttcatccc
    15781 taatcgtgtc gccgcgccac ggggcccggc aacaatgcca ttggtttctc gcaatacctg
    15841 aacaatgcgc tgacgttctt cttcgtcatt ctctggcgtc gtcgggtgca gcaactgcgc
    15901 cattgaactg cgaagcgctg aatcttcatt tagcgtcggc agtaaacggc tttgtcggac
    15961 attaagatgt aaattcagac tgttaccgcg agtcagcagt accgctcgct caatcacgtt
    16021 ttccagctcg cgcacgttgc ccggccagtc ccacgacatc aactggcgta gcgcctcggt
    16081 cgggatggcg tcaattgaac gattcatatg gcgcgccatt ttttgcgtga aatgctttgc
    16141 taaaagaggg atatcttccg gacggtcgcg cagcggcggc aattccagtg ggaagacatt
    16201 caggcgataa aagagatcgc tgcgaaactg gcgatcttca accatttgcc acaaatcacg
    16261 gttggtggcg gcaatgactc tgacatttac cgggatcgtt ctactcccgc cgagacgctc
    16321 aatctcccgt tcctgcaata cgcgcagcag tttaggctga agttctaacg gcagatcgcc
    16381 aatttcatcg agaaacaacg tgccgccatc ggcaatttca aaacgaccac gatgggtatt
    16441 aatcgcacca gtaaacgccc ctttgtcatg accgaataac tcactttcca gaagactggc
    16501 ggggatggca gcgcagttga ttttgaccag cggcttgtcg cgtcgcgggc taagttgatg
    16561 gatcgctctg gcgatcacct ctttgccggt tccggtttca ccgcaaatca acaccgtact
    16621 gtcgctcttc gccacaatat ctacctgctg gagtaggtct tccattgcct ggctttgata
    16681 gataatgtca ccgatgccca gattcgaaag gagctgctcg ctaagctggt ggttttcttg
    16741 ctgcaaactt tcctgcaaat cggtcatgct acgccaggca tcggcattgc caacggcaat
    16801 agcgatgcga tcggctatgt gttgtagtag ctggcagttt tcctcactaa agagagtgga
    16861 agaggtatgc gccagcaaca atgcacccgg tgtatggttg ccaaaggtaa gcggtataag
    16921 gagcgcagat tcgcagccgt tagatgcaag taagagtaat aacgggtcgc gttgccatag
    16981 aaacagatcg tctgcacggt gcgtcaaggt cggttgtcgg gtttgtagcg tttgtgtcag
    17041 caatacactg tcgccaggca tattgcgtgg cagacacgca caatgtgagg cagaaagatc
    17101 gctgcaccac aggctaaact tctcgttctt tcgatgatcg cccagtacca tactgactga
    17161 agccagaccg aaaaaatgat ggatctcacg agcgacgtca gcgatcagat cgtcgagatc
    17221 aagatgtgac agcactgcat tagtaatatc aaccagtacc cgatgatgat cgcgatcttt
    17281 ctccagcaaa cgcagcgcca cctgctgttc ttcatcattg agccatgatt gcagaaccgg
    17341 ggtgattatc cgcgccagcc agttaaacag tgtgcaatcc tggggagtaa aacggctgcc
    17401 gtgggtacga ataaagcgta gctccgccat tccactactg ccgagaagga cggcgtgacg
    17461 cgaataatcg accatctcgc cctgatactc tgttgttgcc gtcacatcgc tgccatcatc
    17521 atggtagcgg taatagaagt tgcttttgag aacatgcagt tcaataagtt gtaccgggct
    17581 gaacggcggc ggcatcggga tgaaggcgcg taataaagac gccttaccgt gtttctgtgc
    17641 taaccgctcc gcgagcattc cgtttaccgc ttcaattgtt attccttgtg gcggggcaaa
    17701 catcgcctcg tctgacatag ccatcaacag tccttattaa tgagaaatcc ttctcacgca
    17761 atttttctgg ccatcagata gatggccggt tcctgcacaa tttcatccaa cagatgaatg
    17821 aattgaatgc tacgcgcctg ccacacagac gtcgcgtcat ccgccagtgg cgcgaatgcc
    17881 cgggtcaaca gggcggtgtg gctggcgtca ataacaatct caccaaaagt catcaccccc
    17941 agcatcttac gtcgggcttg ctcctcttcg acctgggcaa gccaatcttc gtattccgtc
    18001 agtgggcagg ggaaggcgac attcagacaa tcaatcacgc caacgtgatg accgatagcc
    18061 agcgagtaat acattacctg agagctgtgt tccggcatct cgtcgctact ggcgacaaac
    18121 ttttttcgca gcgtccagaa aacaatttcc ccgcactctt cagtcatgta acccccggag
    18181 tacgcgaaag agttgctgaa cgatactatt gagacgtgga tcgtcggcat cgcgcaacca
    18241 ggtgttaacc cgatttcctg taggatcgtc gcttaaatgg cgcaggagcc gatcgcaaat
    18301 ttctcgcccc tgacgatagc ccgccagccg ccgcgcttct cgctcaatgg cgatacgctg
    18361 tgatggcggg atctgcggcc acaacggttg catagtcacc ccggtgggat cgcgataatc
    18421 caccgcgtga atcttctgtt gcagcaaacc gagcgccacg gcgaaaccgt gaatggtggc
    18481 ggccggtgtt ggcgggcagc cggggatcca aacatcaatg gggacaatgg tgtcgctgcc
    18541 gccccagacg ctgtagagat cgtggaaaat ccgccgccgg caccgcacgc gccgtaggaa
    18601 acacagattt tatgatcggg ggcagactca tacgcccgaa gtgcaggcat acgcatcgcc
    18661 cgggtgactg cgcctgtaaa taacaaaata tcggcgtgac gcggtgatga aataacctta
    18721 atgccaaaac gttctgcgtc gaatactggt gtgatggcgg caaaaatttc gatttcacag
    18781 gcgttgcagc cgccgcaatc gacgcgataa acgtaagccg agcgacggat atcctgtagc
    18841 aaatgctgct tcatcttttg cgtttgctcg tccagcgtga tgggctggct gacatgttgt
    18901 gtaagcactg gactcatagc tgctccttag ccaccagcgg tacatctgta tcgtcgttga
    18961 gcagcgtcgc gcgttgtttg cactccgggc agacgctcgc ttgcgcccat aacatttcgc
    19021 ggttttgtgg cgcattttgt tgctgtgcca acaattcagc agccagtgcg acggtttttt
    19081 gcggggcaaa cgggcgttcg caacggctgc aacgttgtag atggaacgtc gcgcgggtat
    19141 agagatcggc tttattggtg acggtcagtt caaagttatt ggtaagctgg atggctctgg
    19201 tcgggcacac ttcttcacaa cgtccgcagt aaatacaacg cctcagatag agctgccagg
    19261 tgcgcgaatt ttgctggtcg tcggtctgga tagtcagcgc atttgccgga caagcacagg
    19321 cgcaggcacc gcaggcaata cattggctgg gcatcaggtc cggtttcccg cgaaagcctg
    19381 ggctgacctc cagtggcgcg aagggatatt tcaccgtcgc ggttccggcg cgcataatag
    19441 ttttcagtaa cttcagcatc ttctgtcctt acttcagcgg cgaacggtta cgatcaatac
    19501 cgtagcgttc gatctcttta tacggcacgg tttttgactg gcgcttgcgc acatctacca
    19561 gcgtcacacg gtcggtacag gagtagcagg gatcaaggct accgataatc agcggtgcgt
    19621 cagaaacggt attgccgcgc agcatgtaac gcaacaccgg ccagttggcg taggtggcgg
    19681 cacggcagcg ccagcggaac aatttttggt tatcaccgag catgctccag tgcacgtctt
    19741 caccgcgtgg cgcttcaaca aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt
    19801 cggtcagcag tggggtatcc ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat
    19861 caaacgtctc tttgacacgg accatcacgc gggagaaaac atcgccgccg gtaaaggtaa
    19921 acagtgtttt tggaatattg ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg
    19981 cgatttgtcg gtcgagaatg ccaatgccct gagtgcgttg ttccatattc ggcgtagcaa
    20041 gcagcatctc taccagctcc gacacgtcgg cgcgcatctc gcgcaccagt ttcagcgttt
    20101 gcagacgttg ctctttgaga atatcgcggc gaacaccacc aatcagattc agaccgtagg
    20161 ttttacgcga cccgatcagc aattccgcca tcgtcatcga cttttcccgc acgcggaaaa
    20221 attgcataaa gccggtatca aaaccaacga aatggcagga gaggccaagg ttaagcaaat
    20281 gactgtgtag ccgttcgact tccagcagaa tcgagcgaat agtatgtgct cgttgcggca
    20341 cctcaatccc cagtgcattt tcaaccgagt tggtataggc cacactgtgg gcaaaaccgc
    20401 aaatcccaca cacgcggtcc gataagaagg tcacttcgtt gtagcccatc cgcgtttctg
    20461 ccagtttctc catgccgcga tggacataaa acaggcggta atcagcatcg acaatttgct
    20521 cgccatccac aaacaagcgg aagtgaccca gttcatcgga ggtgatatgc agcgggccga
    20581 cagggatcac ctgcgcatcg ctgttgccct cattgataaa cggatacgtt tcggaatcag
    20641 tcgtcggttc agggcgcagt cgataatcca tcgcatcttt gcgcagcgga tgcatatctt
    20701 ccggccagtc atcgggcaac accaggcgac gctgatccgg caggccaacc ggaatcagcc
    20761 cgtacatatc gcgaatttct cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg
    20821 acggaaactc ccgactgtcg gcatcgacca gtgccttcac cacgatccag catttttcgg
    20881 ccccttccat tgaaagggca taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt
    20941 tgccaaacag gactggaagc cagccatcat gttgataata aagataatgt acaacgtcag
    21001 gcagcagatt gattttcacc gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca
    21061 gcaccgcgcc ggggaactgc gttttcaggg cggcgagaat cgcttcgcca cgatttgacg
    21121 atgaattaac gttcactgtg tttctccgaa acagatgcgg tgcctgaagg caaaaaatca
    21181 tgccaggtgc tacgttgtgc aggcaggtcg tgcgtccctg agaggactat agtggaagcg
    21241 cccgccagga tcctgatgac aggttgtgga atatgcgttc ccatcgccag catcatgacc
    21301 agcagaatca ccattggcga ggtggtcaac cagccgagat caccccggtt aacggcctgc
    21361 ggcggtttcg ccattaacac ccgcgctgcc atccgtacca ggcccgccag caccagcgtt
    21421 aacagcaata acagcaggac gataatcagc aggtgattac gtgccagtcc ggcggtaatg
    21481 gtcataaatt cgctaagaaa aatgttgaag ggcggcatcc ctgccagcgc cagcgcaccg
    21541 ccgccaaaca gcacggcggt aaatggcatg attttgagca tcccacagac gacgttgaga
    21601 tcgcgcgtgc cgtacttgag cagtacattg ccggaaccgc agaacagcag cgtttttgcc
    21661 agactgtggt ttaaggtgtg cagcagcgcg gcaaaaattc ccagtggccc gccaatgcct
    21721 agcgccaccg cgaccagtcc catgttctcc acgctggagt acgccagcag acgcttaatg
    21781 tcccgctgta ccagaatgaa aaatgccgcc acggcaaccg acaacatgcc gaagatgagc
    21841 aacaaccggt tggggaaatc gctgccgatg gcctggcaga taatgatgta atagcgaatt
    21901 agcaccaaca gcgcgcagtt cagcaatacg gcggagagca gggcgctgac cggactcggt
    21961 gcttcactgt gagcatccgg cagccaggcg tgcatgggaa atagcccggt tttggtgcca
    22021 aagccaatta gcacaaacac aaaggccaac agcattaatg ttgggtcaag caaggacgat
    22081 tgcttaagaa cctcgctcca gaatatcgcc atttctgcct gcggcataac gctggcgacg
    22141 ttggcgtata ccagcacggt accgaacaga ccaaaagcga caccaacagt acaaataatg
    22201 atgtacttcc atgctgcttc cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc
    22261 gagcttaagg tggtggcttc gatcgccgcc cacatcacaa tcaggttatt gctggtaaca
    22321 accagcagca tggtaaacaa aaacagatgg aagaaaccgt agtaatcgca cagcgtaacg
    22381 ggggaaagct cgccgtgtgc cacttcatga cgcatgtagc caatcgagta aacaccggtg
    22441 agaaagccaa tcacaccaag aatggcgagg aacaaaccgc ccagaccatc aatatgaagc
    22501 cacagtcccg cagcgaatat ttctcctgca tcagcggcag tttggaccac ccagagtgcc
    22561 agaatcagca gcagtgtgat ccctaagcta tgtaatacag tcaccgtaca agtcgcagag
    22621 agcccccgtt tccggcaggc aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt
    22681 aaagcgaaca tcacagaata actcatctca ttatcccttc agcgcggtca agttgttcac
    22741 gtccagcgtg ccgtgggtac gccatatttt tcttgccagt aacaccatca caatgacggc
    22801 gaagatggcg tcggtagcga tacctatttc caccagttcc ggtgctcgcc aggcaagaag
    22861 cgccagcacc agatgggagc cgttttccat caggcagtaa ccaaaaattt gccgcaggat
    22921 attgcgctgg ctgacaatgc acagcaggcc aagcagaaaa tgacctaacg ctaccgccag
    22981 cgccggtttc agcccggtag ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag
    23041 caggacaatt aacgccgcga gcagtgccat cattgccgga ccgaataacg ctttttccgg
    23101 gatgttctgg ggaatatttc gtgcagcgta agtcatgatt aacggtacca gcagcacttt
    23161 ggtgataaag gcgctggcgg accagatcag cagttgctct gcggcgaaca ggcacgagag
    23221 agtggcgaaa atagacacca gcaccagcga ctggcaggcg taaaatccgc atgacaggcg
    23281 atagcttttg acgctaatca caaacagcga tgtcagcatc atcagtcccg ccagattatt
    23341 tacgatcata gaaccggtca ttcatcatct ccttactgca accaggtcgc ggcgattacg
    23401 ctggaaatca gcgacatcac aatcagtaca atcaacacca ggcgcattga ccctggcagc
    23461 ggtgcggcat cggcgacggc ctcgctcggt ttgccaggca caacgcgacc aaaccagcga
    23521 ataaaccagg cgaaactggc gaccgattca atcatcagca gaatcatggc gggcagcagg
    23581 atccagtact ccactgacaa cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag
    23641 ccgttgaacg gcggcacgcc ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc
    23701 aacggcaggg tgtgcaatac gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa
    23761 ctcagcgcac ctgctacaag gaaaaacagg cttttagcga acgcgtggtt gacgatgtag
    23821 gcgatgctgc cctccagcgc cagccgcgag ccgaagatgg agagcgacaa accgaagaac
    23881 atccagccaa gttgagtgat ggtcgaccag gccagcaacc gcttcatatc ctgctgtggc
    23941 aaatacatca gaaagccata aagaatggtg accagcgcca tgaccatgcc aacgccgcca
    24001 atcacatgcg ggatattgcc gccgtcgata atggcgcggg caaaaatgta aacgcccact
    24061 ttcaccttcg atgcggcgtg gagataggca ctgatcggtg ttggcgcttc cattgcatcc
    24121 ggtagccacg cttgcatcgg tagctgggcc gatttccccc acgcggcaaa caggatgccg
    24181 ccataaacca gataacgtgc gtcgccgtgt aactcgctca tcgcgctaag cgcaaacgtt
    24241 ccggtctgca aaaacagcgt ggcggcggca agatacaacc ccaacgagcc gatatgagtg
    24301 ataagtaacg cttttagtgc tgaacgctgt gctttatcgc tctggtaata actgatcaac
    24361 gcccaggagc agccgcccgt aatttcaaaa aacaacaact gaccgagcag cgtcgaggag
    24421 agtaccagtc ccgccatcgc gccgataaac aacagcaaaa atgcgtaata acgattcgtg
    24481 ccgttatgcg ggtgttcgcg atttttatcc gtcagataac ccgtggagta gatcgtgacc
    24541 agcaaaccga gaaacaccac cacaaacaga atcagcgtac tcacgcgatc aatgaccagg
    24601 ccaaacaatg ccacatcccc cacgttgacc aatgtcaacg tgacggcaac tttatcggcc
    24661 tgataaaacg cggagatcag tgacaacatg cacagcgtgg tcagcgcggc gaacaaaacc
    24721 ccccattcgg cggcccgacg ttgtggcgaa aacgaaacga ccagtgcgcc gataaaaggc
    24781 agcaataacg tcgtcagagc aagattttcc atattccgtc agtgctcctt acagaccggt
    24841 taaccagaag acccatgcaa gcgcagcaag gctgaagcca agccaggtca catggtgaat
    24901 gagtaaaaaa cgcccgcgtg ccagcgtgtt ttctgcgatt gaggccagta caaaaatcag
    24961 caaaaccttg agcagcgtaa cgacaagtga agtcagcagg caggcgagag aaagttcttg
    25021 cgcgcgccca aaggacagaa acagggccac aaacagtgat gccatcacga cctgtttcag
    25081 ccccagcccc catttcgcta gcgccagccc ggcaccggaa tattcggtca gcgggccttc
    25141 ctgtaattcc tgttctgctt cagcaacatc aaagggaatt tttcccatct caatgaagca
    25201 ggcaaaacca caggccagta acgccagtac ggtggttagc ggcgagttcc agcccatcgc
    25261 cagcgtattg ctgatcatct cgatatgcgt ggaacctgct atcagcgcca ataccagcag
    25321 tgagagaata agcattggtt cgaccagaat gccgagcgtc aactcgcgac tggcaccgac
    25381 tcccgcaaac ggacttccgg tatccagccc ggaaagagca aagaaaaaac gaaacagggc
    25441 aagaagatag ataagggtga tcagatcgcc gccgcccgca aaaggggaaa cggtaataaa
    25501 cagtggtaag gccatcgcca gcaccagcat gctgctgatt aatacccacg gcatcaggcg
    25561 gaacatcaga cctgaagatg tcggcgcaac ttcctggcgt ttaaacagtt tgtggatatc
    25621 gcgataatcc tgccagatcc ccggcccgcg gcgggagtgc atacgcgcgc gtatctgccg
    25681 ggaaataccc gtaaaaagtg gggttagcat cagcagaatc acggcctgtg ctaacgcaaa
    25741 aataaccaga tatccgtcgc aaagagtttg tctcatggtg atttccttag acggcaatag
    25801 cgattagcag cacaaccagt gcggcgacca catacaggca atagagacga aagtcgccgc
    25861 tttgcagatg ctgtatttct ttggccagcc gttgggtggc gctcacgatg gggcggatca
    25921 cccgctcatc ccagaagggt tctgtgctct gagccctggc ggtgacgtgc gcaaaacctt
    25981 tatttagcct cagcgtaggg tcgagctgtt gtcgtagacg aaataacgca gagaagacca
    26041 cacgcagcgg ctgcatcacg ccattgcctg acggggccat cgcattttcc cagccgtagc
    26101 cgcatgccca ggcatctcct gtgcgacgaa acgctgcgcg gcgcgaacga caccacagcc
    26161 agtaaaggcc aggcagcaaa ggcatcgcca gtagtaacag caacagtaat gagggggtga
    26221 cctgtgtatg aaacgtgccg ggtacaagtg ctattccgct ggcgacagtg acgggagggg
    26281 tattggtaaa cgcatgggca atatgcatta tcttcggtgc cagccaactg gcactaagcg
    26341 caattaatac gcagagtgcc gcgagcagta gcatggcgac gatcatcgta tttggcactt
    26401 cctgtgcctc ttcagcgtgt gtactgcgcg gcgcaccaca gaaagtaata ccgtacattt
    26461 ttacgaagca cattactgcc agcccaccag tgactgccag cattaccata gcaataggac
    26521 ccgcaagttg tagcgctacg gcttccacac gacttagtga gaacagcgac tgccaggtgt
    26581 accattcgct gataaaacca ttcagcggag gaatggctga tatcgcgagg caaccaatca
    26641 ggcatgctgc ggctgtccac ggcatccgtt tcgccagtgc ccccattttt tccatgtcgt
    26701 gggtatgcaa acgcgaaata atcgcgcccg cgccgagaaa tagcagccct ttgaacagcg
    26761 catggttgag cagatgaaac agtgcgccga gcagtccaac cacggtgagc agcgggtcgt
    26821 gcagtgacag accgaccatc gccacaccga ctgccagcaa aataatgccg acgttttcga
    26881 cggtactcca ggccagcagc cgtttgatat cctgttccgc cagcgcatac agcacgccca
    26941 ggagcgcgga gattgcgccg atcgccatca ccagaatgcc ccaccacagc ggcaaacccg
    27001 tttgcgccag caggtccatc gctactttca ggatgccgaa aataccgatt ttgaccatta
    27061 cgccagacat caacgccgaa gcgtgcgatg gtgcggcagg gtgagcgcgc ggcaaccagc
    27121 tgtgcaacgg catcatcccg gctttcgcgc caaaaccgaa aaaggccagc aggaacaccg
    27181 ccgacgccag ccccggagaa agtgaaagcg tgcggaaact ggcaaaatcg aggctgccgc
    27241 tttcgcgccc catcagcaag aaggcgatca taatcagcac cgatccggcg tgggcgataa
    27301 aaaagtagag catgccagcg ttgatcgacg ttttatcctg cctggcaatg accagaaacc
    27361 aggaagacag cgacatcatt tcaaacagca cgatgaacca aaaagcgttg tccatcacca
    27421 gcagggcaac catcgatgcg atgaaaagat tcataaagaa gcccatcgcc gccgcgcctt
    27481 tgccctcgta ttcgcgcata taagtcaatg aatagagcga acaaaccacc accagcaagg
    27541 agataaccag caccataaat gccgagaggc tatccattcg cagggtaaaa tcggcaaagt
    27601 caaaaggggt ggcaaaacgg gcgactaaag ttacaccagc atgtaattgc gtaattccgc
    27661 tgatgatgcc aatcacgccg cccaccagcg atgtgatgcc ggaaagctta atagccagtc
    27721 tgtccagacc gagtaaaaac agcgaagcca gactggcaaa cagatagagg atcagcgacc
    27781 aggttaataa ttgcagggca tccattagcg ttgctcctga gtgagggaaa ggggatcctc
    27841 cccgccattg gcaaaccagc ttgcggcaag gcgctgtttt tctttcatct gtcgttgcag
    27901 gctatcgtcg gtgatcagcc gtaacgcctg attcgggcaa gcgcgaacgc aggccggacc
    27961 ttctggcaag aaatcacaca ggtcgcattt caccgcgacg gtctggacac ccggttccca
    28021 gcgcagcaat gcatgttggg gcggcacgtt ttcttcgccg tctttgagtg agccttcagc
    28081 ctgaaaaaca tattgcgcag gggcattcac cggacggctt cctgaagcgc tgattgcgcc
    28141 aaatgggcag accacggcgc aaagcttgca gccaatacag aggctttcgt tgagttggat
    28201 cgcatcatcc ctctgggaga tggcattaac caggcagacc tgcaggcaag gggcttcctc
    28261 acagtgatga cacacgacag gggcagtgag tgttgacgtc ttcgccaggg ccaggcgcgg
    28321 gtgttgctgt aaaccttgcg ttttatgcac gtccgaacag gcagcgagac aggtattaca
    28381 tcctgtacac cacagtggtt cggccaccac aaagcggttc atgtcggctc cttgaaaatg
    28441 atatgaaaca ttgcatttca tattgcataa aggatgccat tctgattagc tcaatatatt
    28501 caataagtta tgtattgact ggattctgaa cggtacaaaa atgatggtca ttaacgttca
    28561 gcaaaaaatg gattcgacac ccgtgtcgca ggggaaagcg agaatgtgat cttgatcacg
    28621 agaagtgagg tatgctggcc gcaagcgcag ccagcacgga atggagcaaa gtaatcaggc
    28681 gtgttctttc agccagttca gcacaacgtc gtggtgattg ctggttttga aatcgtcaaa
    28741 gacatgttcg attttgccat cagcgtcaat caggaagctg atgcgatgaa tgccatcgta
    28801 ggttttgccc atgaaggact tttcacccca gacgccgaat tgttcgcaca cctggtggtc
    28861 ctcatcagac aggagcgtaa agttaagcag ctctttttcc gcaaaacggg agagtttttc
    28921 gggtttatcg gtgctgatac ccagcacatc aacgcccgct tttttcaact catccatgtt
    28981 atcgcgtaag ccgcaggcct gtacggtaca gccgggggtc atggctttcg ggtagaaata
    29041 aaccagaaca cgctgtccct ggaagtcggt caaattaact tgttctccgt cttgatccgg
    29101 caagctaaat ttcggtgcga tatcaccggc tttcagtgga ttcattactt aactccatcc
    29161 tgttcatcat gttgggaata attgacgacg ttaatactgc cttgtgcatt gagttctgta
    29221 catagggctt tgaacgcttg ctcaatattt gctgcgtccg cagatgcggg gctgtgggcg
    29281 gttatctgaa tatgcaactg cgcagcccgt tcattttcag caggttgcgt gcgcgacacc
    29341 agctccgcaa tgttcatatg atgcgcgtcg aaaagtgctg tgaagcgttc aattaaatgc
    29401 ggggagtctg ccacatcgac ctgaacccag acagatgctg gcattggcgg acgcggacgc
    29461 ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc
    29521 aacggtaacg ttgattcaat cagagtgatg gcattccatg aaccggaaag cagcatgata
    29581 aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta
    29641 ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc
    29701 accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc
    29761 ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt
    29821 ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc
    29881 acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct
    29941 agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt
    30001 ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg
    30061 acgctggatc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc taacgctact
    30121 gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg ctgcctgacg
    30181 gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc
    30241 gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat
    30301 ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag
    30361 gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt
    30421 ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg tcatggggtt
    30481 atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca
    30541 gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa
    30601 ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg
    30661 gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc
    30721 agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc
    30781 tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat tactcgctgc
    30841 ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc
    30901 ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga
    30961 ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc
    31021 accagcccag ccgctggcac tggtttctgg tgcgcgtacc cagttcacgg gcgataccgc
    31081 ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct
    31141 gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccaccga
    31201 ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc
    31261 tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc
    31321 gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt
    31381 tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc aaaatcgtgc
    31441 ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccaa tgctggtcgt
    31501 acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat
    31561 gaaagtgacc gacagcaccc gttcgcaggg caacatggcc gtaacttata agccgctgtc
    31621 tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa
    31681 actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca
    31741 tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa
    31801 gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg caaacgtgtg
    31861 cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg tcggacgtag
    31921 ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga
    31981 ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat
    32041 cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc
    32101 cgcaatgata cgtcagcagg ggatggtgcg cgcattgagc agtttgatcg caaaggtatg
    32161 gtgaacaaca agttcaatta cttcattatg agcaaactgg ctgaagcggg tatcccgact
    32221 caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg
    32281 gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa
    32341 gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga cgccatgcac
    32401 gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg
    32461 gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact gttcgatgat
    32521 gctggtctga ttctggtcga cttcaagctg gagtttggcc tgtacaaagg tgaagtggta
    32581 ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa
    32641 atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc
    32701 gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgtttc tttagccgga
    32761 gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt atctcccttt
    32821 gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca aaaagggggg
    32881 gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt accgaaagcg
    32941 gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg tacaagaatc
    33001 atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt gagtgcggat
    33061 tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg atgctgaaat
    33121 tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta catgtcagag
    33181 agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg atggagctta
    33241 tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt gaaggtttcg
    33301 tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc gggcaaatca
    33361 gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg atagcgggac
    33421 atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt gagcgagtgg
    33481 cgggaatcag actagaagac tggagctaac attctctttt acttctaaca ttcgtaaatt
    33541 atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc ctgtggtaat
    33601 cttcctctga accacctacg atcatttcta taatgaatat attgttgagg tatctatgcg
    33661 ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct ccggtggtcc
    33721 gtctatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga ttttactgtt
    33781 agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga ctggacagcc
    33841 ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag ctgcaaaatt
    33901 cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg agaagatggg
    33961 taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca ccggctgcgg
    34021 ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag tttatatcga
    34081 tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg
    34141 gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa
    34201 agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat ctgtgcgtat
    34261 ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc agcaaggcgt
    34321 tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg gcgatgaccg
    34381 tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg gcacttctca
    34441 gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac aatgcaatac
    34501 ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc gcttcacaca
    34561 ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag cggggaagag
    34621 ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga ctggctgtgg
    34681 atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca aactttactt
    34741 gggcgcgagt tccggcatgc ggtattcgac gctcgccacg gctttgatgc cgctgccttt
    34801 gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact ccctgtatgg
    34861 gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg ccctgaccct
    34921 attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga taacgacgct
    34981 atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg tactgactgg
    35041 caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca gctactgacc
    35101 atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc ggcgttggca
    35161 gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca
    35221 acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc ggatgccttg
    35281 ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc catacctgcg
    35341 ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag
    35401 ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt tccgcattta
    35461 caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct ggaaaaaatg
    35521 gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca aggcaatatc
    35581 gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt
    35641 tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg ccggatgatg
    35701 gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc cggagcgctg
    35761 tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg ggcaggtttt
    35821 cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag caatccactg
    35881 gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc gcgtcagcgg
    35941 gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga cctcgactat
    36001 ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg ctgcggtttt
    36061 gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg
    36121 ctgttaccga tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt
    36181 cgcgatgcgc aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga tccactaaac
    36241 gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt cgctcatcgt
    36301 ccgctattaa catcgttagg ttgcttattg cgtctgctac aaaccagtga actggcatta
    36361 ccggcgctgc gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg taccacactt
    36421 aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc gcaggcgctg
    36481 ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca atggcaattt
    36541 tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat
    36601 gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa
    36661 acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg
    36721 ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg
    36781 cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcagtgcagg gtattacgga
    36841 agataatcgc caggcgcgtg tggatgccat catgccgacg tttattgaaa cggtacgcta
    36901 ctggcagaaa cagagcggag taggggctaa cgccacggcg ctaattggtt tctcacaagg
    36961 cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc gcgtcattgc
    37021 ctttaacgga cgctatgcga gcctgccgga gaccgcgtct acagccacga caattcactt
    37081 gattcatggt ggtgaagatc cggttatcga tctggcgcat gcagttgcgg cgcaggaagc
    37141 gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag gccatgcgat
    37201 cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc cgaaacatta
    37261 tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta
    37321 aaagacagat tacttggcct gataagacgc gtgagcgtcg catcaggcat cgttcaccat
    37381 tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc ttacttcttc
    37441 ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc
    37501 gggggaagct ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta
    37561 atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc
    37621 agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg
    37681 atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt
    37741 ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata
    37801 aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc
    37861 tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg
    37921 gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc aagcacctgt
    37981 gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt
    38041 tcacccggaa taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg
    38101 gtcgccggga agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa
    38161 ggtgctgcgc gatgtaccgg attgtcagcc agatgcgggt aggcaacatg cccctgaacg
    38221 ccatgaatgg tgaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacct
    38281 accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgtgcc
    38341 attaacgctt cgacgacttt taccgtaccg ttgtgtgcac tggcttcttc atcagaggtg
    38401 atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg ttctgccgct
    38461 accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgcccgaa taacatgcca
    38521 tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc
    38581 acgtcggtat gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc ccaaaaattc
    38641 tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc
    38701 aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt
    38761 gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg
    38821 gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg acgtttgata
    38881 attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg
    38941 gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct
    39001 aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga
    39061 aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa
    39121 tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatttatga gaataaatac
    39181 tcatttaagg gtgaaaacat tttgcctgca aatgattatc agaaaatata ac
//
LOCUS       DAZUFB010000036        38773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZUFB010000036 DAZUFB010000000
VERSION     DAZUFB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.036"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            260..679
                     /gene="umuD"
                     /locus_tag="ACHY3M_002095"
     CDS             260..679
                     /gene="umuD"
                     /locus_tag="ACHY3M_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS4317012.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSSITISSEDTLDVFGVVIHVVKAMR"
     gene            679..1946
                     /gene="umuC"
                     /locus_tag="ACHY3M_002096"
                     /pseudo
     CDS             679..1946
                     /gene="umuC"
                     /locus_tag="ACHY3M_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(1992..2522)
                     /gene="dsbB"
                     /locus_tag="ACHY3M_002097"
     CDS             complement(1992..2522)
                     /gene="dsbB"
                     /locus_tag="ACHY3M_002097"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFS4317013.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2668..4209)
                     /gene="nhaB"
                     /locus_tag="ACHY3M_002098"
     CDS             complement(2668..4209)
                     /gene="nhaB"
                     /locus_tag="ACHY3M_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFS4317014.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4431..5150
                     /gene="fadR"
                     /locus_tag="ACHY3M_002099"
     CDS             4431..5150
                     /gene="fadR"
                     /locus_tag="ACHY3M_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFS4317015.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5202..6734)
                     /gene="ycgB"
                     /locus_tag="ACHY3M_002100"
     CDS             complement(5202..6734)
                     /gene="ycgB"
                     /locus_tag="ACHY3M_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFS4317016.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7064..8362
                     /gene="dadA"
                     /locus_tag="ACHY3M_002101"
     CDS             7064..8362
                     /gene="dadA"
                     /locus_tag="ACHY3M_002101"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFS4317017.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8372..9442
                     /gene="dadX"
                     /locus_tag="ACHY3M_002102"
     CDS             8372..9442
                     /gene="dadX"
                     /locus_tag="ACHY3M_002102"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFS4317018.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQVAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9828..11564)
                     /gene="cvrA"
                     /locus_tag="ACHY3M_002103"
     CDS             complement(9828..11564)
                     /gene="cvrA"
                     /locus_tag="ACHY3M_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFS4317019.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11659..12573)
                     /gene="ldcA"
                     /locus_tag="ACHY3M_002104"
     CDS             complement(11659..12573)
                     /gene="ldcA"
                     /locus_tag="ACHY3M_002104"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFS4317020.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12673..13284
                     /gene="emtA"
                     /locus_tag="ACHY3M_002105"
     CDS             12673..13284
                     /gene="emtA"
                     /locus_tag="ACHY3M_002105"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFS4317021.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13286..14020)
                     /gene="ycgR"
                     /locus_tag="ACHY3M_002106"
     CDS             complement(13286..14020)
                     /gene="ycgR"
                     /locus_tag="ACHY3M_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFS4317022.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14221..14475
                     /gene="ymgE"
                     /locus_tag="ACHY3M_002107"
     CDS             14221..14475
                     /gene="ymgE"
                     /locus_tag="ACHY3M_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4317023.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14653..15093
                     /gene="ycgY"
                     /locus_tag="ACHY3M_002108"
     CDS             14653..15093
                     /gene="ycgY"
                     /locus_tag="ACHY3M_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFS4317024.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15172..16869)
                     /gene="treA"
                     /locus_tag="ACHY3M_002109"
     CDS             complement(15172..16869)
                     /gene="treA"
                     /locus_tag="ACHY3M_002109"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS4317025.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17189..18607)
                     /gene="dhaM"
                     /locus_tag="ACHY3M_002110"
     CDS             complement(17189..18607)
                     /gene="dhaM"
                     /locus_tag="ACHY3M_002110"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4317026.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18618..19250)
                     /gene="dhaL"
                     /locus_tag="ACHY3M_002111"
     CDS             complement(18618..19250)
                     /gene="dhaL"
                     /locus_tag="ACHY3M_002111"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4317027.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19261..20331)
                     /gene="dhaK"
                     /locus_tag="ACHY3M_002112"
     CDS             complement(19261..20331)
                     /gene="dhaK"
                     /locus_tag="ACHY3M_002112"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4317028.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20559..22478
                     /gene="dhaR"
                     /locus_tag="ACHY3M_002113"
     CDS             20559..22478
                     /gene="dhaR"
                     /locus_tag="ACHY3M_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFS4317029.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22578..25463)
                     /gene="ycgV"
                     /locus_tag="ACHY3M_002114"
     CDS             complement(22578..25463)
                     /gene="ycgV"
                     /locus_tag="ACHY3M_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4317030.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26304..27395)
                     /gene="ychF"
                     /locus_tag="ACHY3M_002115"
     CDS             complement(26304..27395)
                     /gene="ychF"
                     /locus_tag="ACHY3M_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4317031.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27512..28096)
                     /gene="pth"
                     /locus_tag="ACHY3M_002116"
     CDS             complement(27512..28096)
                     /gene="pth"
                     /locus_tag="ACHY3M_002116"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4317032.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28374..28652
                     /gene="ychH"
                     /locus_tag="ACHY3M_002117"
     CDS             28374..28652
                     /gene="ychH"
                     /locus_tag="ACHY3M_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFS4317033.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28707..30386)
                     /gene="dauA"
                     /locus_tag="ACHY3M_002118"
     CDS             complement(28707..30386)
                     /gene="dauA"
                     /locus_tag="ACHY3M_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFS4317034.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30511..31458)
                     /gene="prs"
                     /locus_tag="ACHY3M_002119"
     CDS             complement(30511..31458)
                     /gene="prs"
                     /locus_tag="ACHY3M_002119"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4317035.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31609..32460)
                     /gene="ispE"
                     /locus_tag="ACHY3M_002120"
     CDS             complement(31609..32460)
                     /gene="ispE"
                     /locus_tag="ACHY3M_002120"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4317036.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPSVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32460..33083)
                     /gene="lolB"
                     /locus_tag="ACHY3M_002121"
     CDS             complement(32460..33083)
                     /gene="lolB"
                     /locus_tag="ACHY3M_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFS4317037.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33297..34553
                     /gene="hemA"
                     /locus_tag="ACHY3M_002122"
     CDS             33297..34553
                     /gene="hemA"
                     /locus_tag="ACHY3M_002122"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFS4317038.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALILWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34595..35677
                     /gene="prfA"
                     /locus_tag="ACHY3M_002123"
     CDS             34595..35677
                     /gene="prfA"
                     /locus_tag="ACHY3M_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4317039.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35677..36510
                     /gene="prmC"
                     /locus_tag="ACHY3M_002124"
     CDS             35677..36510
                     /gene="prmC"
                     /locus_tag="ACHY3M_002124"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4317040.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36507..36899
                     /gene="sirB2"
                     /locus_tag="ACHY3M_002125"
     CDS             36507..36899
                     /gene="sirB2"
                     /locus_tag="ACHY3M_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFS4317041.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36903..37712
                     /gene="sirB1"
                     /locus_tag="ACHY3M_002126"
     CDS             36903..37712
                     /gene="sirB1"
                     /locus_tag="ACHY3M_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFS4317042.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37748..38602
                     /gene="kdsA"
                     /locus_tag="ACHY3M_002127"
     CDS             37748..38602
                     /gene="kdsA"
                     /locus_tag="ACHY3M_002127"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFS4317043.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         9632 a         9655 c         9987 g         9499 t
ORIGIN      
        1 tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc gaatgcttct
       61 attctatttt ttagccgggt aatatttttc atttctgctg gatgagcgta gtcgccagaa
      121 ggccgcgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc gacaaatatt
      181 gatagcctga atcagtattg atctgctggc aagaacagac tgctgtatat aaaaacagta
      241 tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg aaattgtgac
      301 ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag cagattacgt
      361 tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga cttacttcgt
      421 caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt tactgattgt
      481 cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg acggcgagtt
      541 tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga acagtgcgta
      601 ctcgtccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg tgatccacgt
      661 cgttaaggcg atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta tgccagctgt
      721 gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc gaataatgac
      781 ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat gggcgatccc
      841 tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag cagcaattat
      901 gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact atcgccccgc
      961 gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg taattgtcgc
     1021 gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac ccatcttact
     1081 gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca tgcggcaaaa
     1141 aaatggcagc ggcagacggg ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat
     1201 taatgtctgc tctccccgtg gatgaagtct gggggattgg acggcggatc agcaaaaaac
     1261 tggacgcgat ggggatcaaa accgttcttg atttggcgga tacagatatc cggtttatcc
     1321 gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggc gaaccctgtt
     1381 tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg
     1441 aacgcatcac ggattatcca tcgatgcggc aggccatttg tagttacgct gcccgggcgg
     1501 cggaaaaact tcgcagcgag catcaatatt gtcggtttat ctccacattt attaagacgt
     1561 caccatttga gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc
     1621 ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat gccatctggc
     1681 aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc agtcagggag
     1741 tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag caattgatga
     1801 cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt gccgggcagg
     1861 ggatccagca acaatggcag atgaagcgtg ccatgctttc accacgttat acaacgcgaa
     1921 gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct cccgcaggag
     1981 cgccgaatgg attagcgacc gaacaggtca cgttttttcg ctttaaacgg ctgggaaatc
     2041 accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca ctgcggcatt
     2101 tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac aaacacttgc
     2161 ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc acaggtggca
     2221 aacggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac accgcggaac
     2281 gcactataca accagataac catcgctaca taacgcagcg gagttttcgg ggcgatcgcg
     2341 ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat acagagcacg
     2401 caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag tgccagagca
     2461 gtaaacgcca tcaacaacca cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac
     2521 ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca ttcgctgcgc
     2581 caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg
     2641 ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca tttgcataaa
     2701 ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga gtgtcagtac
     2761 gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa tcaatggcgc
     2821 gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag cgacggaggg
     2881 cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca acgtgatagc
     2941 gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga agacgttatc
     3001 cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag catgttccga
     3061 tgcctgcaac acaaactgaa taattggcga aaacagttgt tggtcgataa tcaccgcgac
     3121 taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga aggctttacc
     3181 gatagcatgt tcatcggtga caccggtcaa tgatgtcgcc agaataatga ctgacaaacc
     3241 aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga cgccaataat
     3301 cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc tttgatcgtc
     3361 aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc caaaccaacg
     3421 cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa cggtcaccgg
     3481 cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga tgatcaggtt
     3541 ctgtggttcg cccaccatgg tcatcacgcc gcctaatgca gtgccgacac cggcatgcat
     3601 catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat gcttgtcgat
     3661 atgactatcg tcttgcaggt cggtatcttc agtacgggaa gaggctacgc ggtgataaat
     3721 accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg catcgaggaa
     3781 cgcggagagg aacgccgccg ccacgcaaaa agagagcgac agcagcattt tggagcgaat
     3841 gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat agatacccgc
     3901 caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt cacggacgtg
     3961 ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac cggggagcag
     4021 cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca gcaaccagcc
     4081 cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa tgatgagggc
     4141 gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc ggccccagga
     4201 gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta tgtttaaacg
     4261 caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg aaagagatgc
     4321 ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg tagttagccc
     4381 tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact atggtcatta
     4441 aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
     4501 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa
     4561 cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc
     4621 aacatggcaa gccgacgaag gtgaataatt tctgggaaac ctctggttta aatatccttg
     4681 aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat ttgctgtcgg
     4741 tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat cccgataaag
     4801 cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc tttgccgagc
     4861 tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
     4921 tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc gccaatccgg
     4981 aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg
     5041 cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc gagatttggc
     5101 accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa tcccttccgt
     5161 ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt tcccattctt
     5221 ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc gagcatcaca
     5281 tcaaatcccc acaggcgatg cacatgcttc agcacttctt tgcgcccccg atccagcggt
     5341 gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc
     5401 cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa ccggttacgg
     5461 atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt atgccgatca
     5521 tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag aaactggctg
     5581 ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa
     5641 ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca aatccgttta
     5701 atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta ccacgggctg
     5761 ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag cataaaacgt
     5821 tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca gaaggtcgcc
     5881 cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct caccttacgc
     5941 acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt ctccataaaa
     6001 tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac cgttttctct
     6061 tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag atactcttcg
     6121 cgacttttct gccgggcttt ctcttcttgc agtgagattt tttgcgggcg tttgtaccgg
     6181 tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac ttcatcaacg
     6241 ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata atcgacaatc
     6301 gaactggcgt cggtccaact gcggaataag taattgtttt taaagaaaga gttatgccca
     6361 tagcaggcgt gtgccatcac cagcgcctgc atggtaatgg tgttctcttc catcaggtaa
     6421 gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg accgtgctta
     6481 tacagccgtt cagtttcgat aaacttttta ccgaatgacc agtgcggata gttaattggc
     6541 atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc aatctggtgc
     6601 gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag ataaacatcc
     6661 agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa
     6721 tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg agcacctcat
     6781 ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc tttgcgattt
     6841 cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg ataaaaaggg
     6901 cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga tgtgagccag
     6961 ctcaccataa aaaagccgta tattgaataa tattttcaac tgagttatca agatgtaatt
     7021 agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag ttgtcatact
     7081 gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag gacatgaggt
     7141 caccgtcatt gatcgggagc cgggggccgc cctggaaacc agcgccgcga atgccgggca
     7201 aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa aagcgatcaa
     7261 atggatgttc cagcgccatg cgccgctggc ggtccgtctc gacggtacgc agtttcagtt
     7321 gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg aaaacaaagg
     7381 gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc gcgccgaaac
     7441 caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca
     7501 gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac cgtatcagct
     7561 gctggaatcc agccgcctgg cggaagtgga gcccgcgctg gcagaagtag cgcacaaact
     7621 gacgggtggt ctgcaactgc ccaatgatga aaccggagac tgccagctgt ttacccagaa
     7681 tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc ccgttgacca
     7741 actgctttgc gacggcgagc aaatctacgg cgtgaagtgt ggcgatgaag tgattaaggc
     7801 cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag gcattgttga
     7861 tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc aggaagatgg
     7921 tgcgccagta tccactattc ttgatgaaac ctacaaaatc gccattaccc gtttcgataa
     7981 ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc tgttgcaacc
     8041 gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg gtcatgtcga
     8101 gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc cggttgtcgg
     8161 gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg gctggacgat
     8221 ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc cagcgatccc
     8281 atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac gtccgggcca
     8341 tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg ccagcctcga
     8401 tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc acgcgcgcgt
     8461 ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct ggagcgcgct
     8521 cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt tacgtgagcg
     8581 cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg atctggagat
     8641 ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca aagcactgca
     8701 aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg
     8761 gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg caatggcgaa
     8821 tgttggcgag atgaccctga tgtcgcattt tgccgaagcg gaacatcctg atggaatttc
     8881 cagcgcgatg gcgcgtattg agcaggtggc ggaagggctg gagtgtcggc gttcgttgtc
     8941 caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc ggcctggcat
     9001 tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata ccggattacg
     9061 tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag cgggtgagcg
     9121 tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc
     9181 agggtacgcc gacggttatc cgcgccacgc gcctaccggt acccctgttt tagtggacgg
     9241 cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctagcggtcg atttaacgcc
     9301 ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga tcaaaattga
     9361 tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc tggcgttacg
     9421 cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg tcttctggtt
     9481 gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga cagagccatt
     9541 gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg aactcaccgc
     9601 acaggcgttc tacataaaac gcttacgctt cattgttgac tcgaactcga cttcagataa
     9661 atcacgcttt acccttgatg gagcctgtac atagatttgt gtaattgcct gattttgata
     9721 tgttcaattc aacatcaaat gaaggttaaa ttatggacga caaacaattg caggctcagg
     9781 ctgcgttcag caaagcatcg caaccggcga tagatgcttc attaaattta agattcagct
     9841 tcctcttcag ccacccgtac gccaatcttc aacacttcat tatcttcttt ctcggccacc
     9901 gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgc gcctaacaac
     9961 tgctggacaa tttcacccag cgtttgctgc ttatcacgat attctcgccc gtcttccagg
    10021 ccatatatca gcgcaacatc agcatatttg gcgctggctt cgagaatgaa gtcaccaaag
    10081 aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag cgccggaaga
    10141 tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt gctgccggta
    10201 ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt tggcatatgc
    10261 aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata aacaaactgc
    10321 tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg tcccactggc
    10381 ggaaccacca ctttggcttt tttagccgcc cacgagagtg atgttccctg caagagcagt
    10441 gaaaccagaa ccacaaagaa ggcgacatta aagaacagac gtgcattctc cagccccgcc
    10501 atcatcggga acacagccag gatgattggc accgcgccgc gtaatcctac ccagctgata
    10561 aacacgcgct cacgcagatt gaaaccgcgg aaggggagca atccagcaaa taccgaaagg
    10621 ggacgggcga agaatatcat ccatgccgac aaaatgagcg ccggaatggc aataggcagc
    10681 agatcgcttg ggttaaccaa cagccccagc accaggaaca tggcgatttg cgccagccag
    10741 gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg attacccagc
    10801 agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt ggttaaagca
    10861 aaaattaaaa taccgccgct taatgccagc aatggatata atccggcggg cagggcgatg
    10921 cgattaatca tttgcagcag taaataacca ccaccaagtc cgatgacaat cccgaggcca
    10981 aattgctgca gaatatcgac cacgaacatc caactgacac tgctttcatg ttgctggatc
    11041 atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc ggattcaatt
    11101 tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga aaagaccgct
    11161 gcagcatcgg tagagccgac gatagcgccg attaataagc cttcaatcaa atcaagatta
    11221 aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc cagcgtcgcc
    11281 agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg catcccgccg
    11341 tcgagcaaaa taatcgccag tgccaggtta cttaccatgt aggcgaaggg gtaattatca
    11401 aacgggatac cgccgacgcc atcgactcct gccagcatgc cgatcgccag aaaaataacc
    11461 agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct ggtgacaagg
    11521 atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg gttactcctg
    11581 attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg ttttacttag
    11641 ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg tcaactgagt
    11701 gccttcccgg gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa ccgtgcgttg
    11761 ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc
    11821 gtacactgac tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct
    11881 accgagaata atcgccttct gacgtggcaa aattcccgca tgatagagct gcaacagcat
    11941 acgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac cgttctcaat
    12001 ttttggcatc cacggtgtac caatcagtga aatcagcatc gcaagattgc ctccccacaa
    12061 cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga aggtttcatt
    12121 gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc agttcatccg cgccaaagtt
    12181 tgctaccagc atggggccgc taaaggtgat gacattgcct tgcgccagaa gaccgcactg
    12241 aatggcggta aaatcgctat gcccgcatat gagcaacggg tcatattgtt ggcgagcaac
    12301 cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc
    12361 cagcacgatg gtgttggggg tggtcagtct ggcaagggaa ttgagatctt ccaggcgctc
    12421 cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt tattgacctg
    12481 atgcccctcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt taatgcagta
    12541 acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac aacagaaacg
    12601 aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt ccaggctata
    12661 aggatagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt agcgggttgt
    12721 tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt gcaacgtgcg
    12781 atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg
    12841 atcacggcga ttatcgctat cgaatcgggt ggtaatccga atgcggtgag taaatcgaat
    12901 gccattggtt tgatgcagtt aaaagcttca acctccggac gtgatgttta tcgccgtatg
    12961 ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc cagagcgtaa tatttcaatg
    13021 ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga agatccgaag
    13081 gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct gctacggact
    13141 ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atttggatgc tgacgagttc
    13201 ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat ctacaaactt
    13261 gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt gtccgctttt tcccgggctt
    13321 ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg ctgacgttaa
    13381 gaaaacggaa gctcagacgg ggagtggtga tggtttcatt cttgccatca atcactttgc
    13441 gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc cccatgttga
    13501 cttcaatctg agcgaagcgc atgccttctt gtaattcggc aggctttgct gtttccagta
    13561 atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta ctgttatccg
    13621 ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg aaatatcggc
    13681 gtcgttgtac aaaccataag gtgggaggcg gtacggtgat aaatgccgga agctgcaagt
    13741 attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga gtttcggcgg
    13801 taatggtaat gtgctgtgcc tttagcacgg cgatgttgtc ttcggcttga ctgccgaaat
    13861 ccagcaccag tttatccggg gttattgcca gtattttgct gatcagctgc ccgccattcc
    13921 aactgagacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc aggacggcta
    13981 acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac tcctgactga
    14041 caaactgtct ttggtttatc ggtcacagtt aacaaaactt aatacaaatg cgtgaatatt
    14101 ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgatcccg cccttgccta
    14161 tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga gggcctgaac
    14221 atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc caagctaatc
    14281 atgccggggc gtgatggtgg tggatttttc ctgacctgta ttctcgggat agtcggtgcg
    14341 gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag tggttttaat
    14401 ctgcacagct tcctggtggc ggtggtggga gctattctcg ttctgggcgt attccgcctc
    14461 ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc tatccgcttt
    14521 cacatcagaa cgtatactcg acacctgctt tacgggtgga acaaatcaat atagcactta
    14581 gatgcccgat aaagtcggtt cccggtaaca acaacagaat ccttttttaa ttattgtttc
    14641 gttgtttttg gtgtgattca gaaaataaat attttagaca gtagccagtc aggcgttaaa
    14701 aatggggcgg atagtgaggc ggttttggct gcgcttattg aacatttaga attgatcaat
    14761 ccttctggca gattaagcca aaatacccgt tcggcaatgc ttcagttacg cgaagaggaa
    14821 tggtctgaat tctttttttg gttgctaaat tctctggagt gtctggatta tgtgattatt
    14881 aatttaactc cagaaagcaa aaaaacgttg atgagtgaac accgtaacaa tattcaggta
    14941 gcaatcgatg ctttatatag gcaacgtcgg tgtaaatcac cgggtgatga aagtgaaaca
    15001 cttacccgta gaaatgacgc tatcttcggc aaccacgtat ggcaaacatt tgcgcaatac
    15061 ttcccgccag gacttgaaaa accgtctgtg tagaaaacac catagctatg gtgttatctg
    15121 ccgacaacgc catccggaga agccgaatga cgttgtcgga gtaagcgctg gttaaggtgt
    15181 gggttgtgcc tctttggttg agggttgcgt cgttgctgac ttaacggtcg gacgcgtcgc
    15241 cggaacattg tcacacggtt gctctttcgg gcagatcaaa tccagcattt tcagcgtcac
    15301 gccattggtc cagccaaagc catcctgtaa tggatattcg ccaccgccgc cccccgttcc
    15361 ggtggtgctg acatcatatt tttccaccag ctttttctcc cggtcatagg tgtgctgaac
    15421 attggtcagg aagtgccagc taatgtccat cgccacctct ttttgcccgt agttttgtaa
    15481 tccttctgtc gcgacccact gtaacggtgc ccagccattt ggcgcatccc attgttgccc
    15541 acttttcacc gacgtggtgt tcaggccgcc gggttgcagc agatgtgttt tcgtcgccgt
    15601 cgccattttg ttggcgcgat ctttcgctgc cgcattgacg tacagcggga acagggcggc
    15661 cgcggttaac tgattgcgca ctttatgact tttcaggtcg taatcggcat accagccttg
    15721 ttgatcgttc cacatgtatt tttcgatccc tttttgacgg gcatttgcca gcgtttcgta
    15781 ctggtttgcc atcgcgttat ctccggcagc tttgctggcg cgggcgagga ttttttccat
    15841 tttaaacatc aggctgttca gatcgaccgg tacgatgctg gtggtgcgta aggtatttag
    15901 ttgatgcggg ttgtccatcc agcgcgagct gaaatcccag ccagacgcag cggcagagcg
    15961 caggtcgcgg taaatttcag tggcaggtcg attcggattg cttttggcgg tggcaatatc
    16021 ttccacccat gactctggtc gtggcgtatc gcgatcgtcc cagtagcggt tgagaagggt
    16081 accatcctga agttttacaa cgcgtttttc ctgttgtccg gcttgcaggt tttcaacacc
    16141 gtccatccag taagcatatt ctttttgcat ttgcggcagg tattgcttca acgcggcatc
    16201 gccttcatgc tgcgccagta actctaccat cagggcaaag aagggcggtt gcgagcggct
    16261 taaatagtaa ctgcggttgc cgttgggaat atgaccgtaa gtgtctattt catgagcaaa
    16321 attggccacc atatccgcga ctttatccca gtgaccgctt tcggcaagtc ctaacatggt
    16381 gaagtaactg tcccagtaat atacctcgcg aaaacgtccg cccggcacga cataaggttc
    16441 cggcagcggt aacagagaat cccatttttc ggtgttttcg gtagaacgcg ttaataccgg
    16501 ccaaagtccg tcaatatgtt cgcgcagtga ctgcccctct ggcggaacat atttctcgcc
    16561 ctctttcggc agggtgaaat tgacgttaac gaaatggcgc agatcaaatc cgctctggtt
    16621 ttgctgcatc cgataatcag caaggatcat cagcggatcg ctgttcggca cggcatcggc
    16681 aaaggttttt tggtccggaa aaagtttggc gttttgcaca tcattaaaca gcggccctaa
    16741 taaaatatca ggcggctgtg gtgttaccgg tgtttcttct gcctgcaccg atagcgcagc
    16801 gaaacacaaa aagatacagg ctggaattaa cgccattttt tgcgggcgag aaggtgcggg
    16861 ggatttcatc aatcattctc ctttggcgaa accgaataaa acggtattca agtatcagaa
    16921 aaccttagtt caggatcgca ctagctgcat gatcgaaatc tcattctgcg aactgtcagg
    16981 cattttagaa aagggaatta aagggataaa taacggtgaa atagagtgat caaataacgt
    17041 ctgtttatta gccagtcagc gacgttgggt tcgcgattag cgaagaaaaa tcatcataaa
    17101 ttggtaaaaa atataacagg tagttaaaaa acatcagtgc tgggtaaatt gccggatgac
    17161 atcagaacga tgccatccga acggtggctt aaccctgacg gttgaaacgt tgcgttttaa
    17221 cgtccagcgt tagcgtttct tctggttgta tcgcatacag tttctcaccc tgctggcaaa
    17281 tccagccaat ccccagttca cgggcgatta gggcgctgtg ggataccgga cttccggcgc
    17341 taaggcagat accttttaca accgccggat ccagttgcag tactgtggaa ggataaatgt
    17401 tctccgccag tagaatagtt ggcgagttaa actgcgggag ttcttctttc gtttgggtca
    17461 ggtggaccag ggtgcgatgc agaagatcgt ccacatcaat atagcgagct tgtagatatt
    17521 catcatccag ttgctggtat tgctggctaa gttctttaag aacttgctgc caggcatatt
    17581 ctgccgtgca atgttcatgc tgaaggagtt cgcttgccgc cgccagcagt tccggatcat
    17641 ctaacagtgt atggtgacca gaaaagattg cggcaatatc gtcaagcccg ctggcttctg
    17701 ctttcgctgt taacgtcatc agatctaata acgtgaagtc aatagcctgg cgtaatcgat
    17761 cttgttcttc ttccacggtc agggttgatt ttgcctgtac cgtacataaa actggttgat
    17821 aataaaaggc tttacccgaa acaggcggaa cgggacgcag agtaggtgga gcgacttcct
    17881 ccgtttcacc aaagttatct tcagccagct gacggaaagc gatcagtgcc tcttcagctt
    17941 ctggcccttt cgcaatcagg cgcagcgtat cgttatagcg aacttgtagt aacgcaatct
    18001 ggttaatact ctctggtgtg acgcatttgc cgtttttttc cagcaacata tcggcattaa
    18061 atgtcgataa ggtataaacc agccgggagg ccggacgtac atgcaggccg ttacggtttt
    18121 ttatgaccac cgccagagaa cgggcttctt catcgtacgc aggacatgtg tcagagattt
    18181 cagtgtcgga ggacggtaaa cccagttgtt cacgtttggc ttccagcgca tgcatagcgt
    18241 caaagataac tttgtcgata tccgctcccg aggccgcgct gaccgttgct gccagtgtac
    18301 cttcgaccaa cggcgcagca cacaaacgta cttttgcggc gatcttggga gccagcaatt
    18361 ccagcgcagt ttcagcactc aataatgcgc tacccatatc catcatgacc agcacatggt
    18421 cggcatcagc aacagattcg atggcctcca tcactttgac ggcatcggta ccaataggat
    18481 tttgtggatc gtcaattccc gcggcaatgg cgattttaca actatcactc attaacatct
    18541 gacgggctaa ttcaccgaca ccttctccca gtcggctgct atgtgaaact atgaccaggt
    18601 ttaccatcac caattcctta ctcttttgcg gctaacgcca acatttgcat cataaacatc
    18661 accgaggtcg cgccgggatc ctggtgacca atactgcgtt caccgagata actggcgcgg
    18721 cctttgcggg cttgcatcgt aatcgtactt tgtgcagcgg attcggcgat tctactggca
    18781 gcttcgagcg ccaccggaac agagagattt tgctcgctgg actgacgtaa cgattccacc
    18841 accggcaccc acacatcaca catggtttta tcgccaggtt cggctttccc gcgactgatt
    18901 acgccgtccg cgccatcgcg gaacatctga taaagctctt ccagtgtcag gctttgccgt
    18961 gcctgggtcg cctgtgcggc gcggataaag aaggtaccga acagcggacc actggcacca
    19021 ccgacgctgg aaagcagcgt cataccggta ttcttgagaa tgaaaccgat atctttatct
    19081 gcgatagcag ggagtttttc caccactttg ctaaagcctc ggttcatatt tagcccgtgg
    19141 tcagcgtcgc caatttcgcg atccagtccg gtaagatact cgctctcggt gctgaaaata
    19201 tcgccacaac gagtgagcca gttaacaatt tgagttctgc tcagtgacat tgctttctcc
    19261 ttatttaccc cagttaaggg ccggggtgtg gaccggggcg tcccagagtg ccagcgtttc
    19321 gtcatcaact ttcagtaagg tgattgagaa accggtcata tccagtgagg tgcagtacgc
    19381 gccaattaaa ttacgttcga tagtcaatcc cgcttgctgg caacgtgtgg tcaggcggtt
    19441 atagacgccg tacagctcag aaagcggagt tgcgccaaga ttgttaacca gcgcaatcac
    19501 ccgatcgcca gactggagcg gttgtttggt ttgttgttct tcctgccaac tgccttgttg
    19561 ataatcccag aaacgcaaag tgcgatggta tgagccattt tccagcaggg tgtcgaacat
    19621 ttcatcgacg gtttgatcaa gggaagagaa ggagcggcgg tcaatacccg gctcaccatg
    19681 aatgccgacg ccaaactcca tctcattatc cgccagggta aaagaaggtt tgcccgcggc
    19741 aggaacggta caggcaccga gagcgatacc tattgagtgg ccttgattat tcaacttacg
    19801 ccccagttcc gcacaggcgt ccagtgagtc gccacgctcc gccgctgcgc ctacgagttt
    19861 ttcaattaat acggtgttgg caacgccgcg tcgcccggcg gtataaagac tgtcttttac
    19921 cgcaacgtcg tcatcaatga ccacagtggt cacttttacg ccgctatcgt gcagtaactc
    19981 ggtcgctgtt tcaaagttaa gaatatcgcc ggtgtaattt ttgataatca acagtacacc
    20041 ttcgccgcca tcaatttgca tggagcattc aaagatttta tcgggcgtcg gtgaggtgaa
    20101 aatttcgccc ggacaggccc ccgaaagcat cccctgaccg atataaccac agtgcatcgg
    20161 ctcgtgtccg ctgccgccac ccgacagcag ggcgactttt cctgcaacag gggcatcagc
    20221 tcgggtgaca tacaccggat cctgatgcag tgtcagcgat ggatgcgctt tcgccagtcc
    20281 tgccagttgt tcgtccagta cgtcttgcac atcattgatc aattttttca ttattttgct
    20341 ccagcaatta cggtagggca tggatgatgt tcaacgacac ggcgaccact gactgccgat
    20401 gaatccattg tgcatcaggc aagggaaaag aaaattagcc caaattatgt ttcatagtga
    20461 aacatatgct ttaatgaatg ttccatattg aaacttttac gtgtattaat acttaaaatt
    20521 gcgagccgga acaccttttg tcataaggga tgcgggatat gagtggcgct tttaacaacg
    20581 atggtcgggg catatctccc ttaattgcaa cctcctggga gcgatgcaat aagctgatga
    20641 aacgggagac atggaacgta ccacatcagg cccagggcgt gacatttgct tctatttatc
    20701 ggcgtaagaa agcgatgctg acgctcgggc aggctgcgct ggaagatgcc tgggaatata
    20761 tggcaccgcg agagtgtgcg ctgtttatcc tcgatgaaac cgcctgcatt ctcagccgta
    20821 atggcgatcc gcaaaccttg cagcagctaa gtgcactggg attcaatgac ggcacgtatt
    20881 gcgccgaggg aattattggt acttgtgcgc tatcgttagc ggctatctct ggtcaggccg
    20941 tgaaaacgat ggccgatcaa catttcaaac aggcactctg gaactgggcc ttttgtgcaa
    21001 cgccgttgtt tgacagcaag ggccgattga cgggaacaat agcgctggcg tgtccggttg
    21061 agcaaactac cgcagctgat ttgccgttga cgttggcaat cgcccgcgag gtcggaaatt
    21121 tactgctgac ggacagtttg ctcgctgaaa ctaaccgtca tttaaatcaa cttaatgccc
    21181 tgttagaaag tatggatgat ggcgtgatta gctgggacga gcagggtaat ttgcaattta
    21241 ttaatgccca ggcggcgcgg gtcttgcgcc ttgacgcgac ggcaagtcag ggaagggcaa
    21301 tcactgaact cttaacgtta cccgccgtat tgcaacaagc aataaaacag gcacatccgc
    21361 tcaaacacgt agaagcaacc tttgaaagtc agcatcagtt tattgatgcg gtgataaccc
    21421 ttaaaccgat aatagaaacg cagggaacca gctttatttt gttgctccat cctgtggaac
    21481 agatgcggca gttgatgacc agtcaattag gaaaagtcag ccataccttc gctcatatgc
    21541 cacaggacga tccgcaaacc cgccgcttga ttcattttgg tcgccaggcg gcgcgcagta
    21601 gctttcctgt cctgctttgt ggagaagagg gcgtgggaaa ggcactgcta agtcaggcaa
    21661 ttcataatga aagcgagcgt gctgcaggtc cttatatcgc cgtcaattgt gagttatatg
    21721 gtgatgcggc gctggcggaa gaatttattg gtggcgatcg cacggacaat gaaaatggcc
    21781 gtctgagtcg gctggaactg gcacacggcg gcacgctgtt tcttgaaaag attgaatatc
    21841 tggcggtgga gttacagtct gctttgcttc aggttatcaa gcagggtgtt atcacgcgac
    21901 tggatgcgcg gcgtttaata ccagttgatg tcaaagtgat tgccacaacg accgcggacc
    21961 tcgcaatgct ggtggaacaa aatcgtttta gtcgccagct gtattacgcg ttgcatgcat
    22021 ttgaaattac catcccgcct ctgcgtatgc ggcgtggcag cattccggcg ctggtgaata
    22081 acaaattacg cagtcttgaa aaacgcttct ctacgcggct gaaaattgat gacgatgccc
    22141 tcgctcgcct ggtttcctgt gcatggcctg gcaacgattt tgaactttac agcgtcatcg
    22201 agaatcttgc tctgagtagt gataatgggc gcattcgcgt cagtgatttg ccggaacatc
    22261 tgtttaccga gcaggcaaca gatgatgtca gcgccacacg cctttccacc agtctgtcat
    22321 ttgcggaagt tgaaaaagag gcaattatta acgcagccca ggtcacaggc ggtcgcattc
    22381 aggaaatgtc ggctttactt gggatcggcc gcactacgct gtggcggaaa atgaagcaac
    22441 atggcattga tgcagggcag tttaagcgcc gggtatgaaa gacagagacg atttctgata
    22501 catcagagtg atctatattt cattccggcg cacgctaaca tttttcagca tcgtttaagg
    22561 gctggtctat cccgcactta aaagctgaag cgatatcctc cgttgacttg cttctgatca
    22621 aagaggttac tctgcgtgta atccgcttcg aggtagaatg tgtgctgttt gttatactgt
    22681 gcactgactc ccacgccgtt attccagcca ttacctttga aactgtactt ctcccgggag
    22741 ttgttcaaca gatagtctgt atcccctgaa aactcgcgga tagctccagt cttcacatac
    22801 atattcagct ggctgttgcc tgcggtgatg tcatatccca gtatcatgct ggcacgcccc
    22861 agcagcgatt cgtagtgatt cagatgtata ttgagaccat tactcgcctt catagccatc
    22921 tcattctggt ggctgtatgt gagctgggtt tgcggctcta tatagaaccc ataaccagta
    22981 ggggtcaggt tgaacctctg cccggcttcc agggagatgc tcaatccatt cgcagtgccg
    23041 ttggcgttaa cgccgttgtt ctgactgtcc agtacgtgaa aactattttt ctggcgcgat
    23101 gcttttacaa cgagatcgct gtaaaaaccg ttgtgtgcca tgtaactggc atacattccc
    23161 atgtagtctg aacgtgcggt accgtcgcct ccgctatagt ccggcgatgc atgtgttgag
    23221 ccaatataca gaccgacata caacggcatt acatcagaga gacgtttatc cccaccaaac
    23281 tggataccgc tgtaacccat gtcaaagccg ctcagtttgc cactggcgaa ggagtccagg
    23341 cttcccccat aactgcgcag ccagatatta ccgtctttac tctgatttcg caggtcaccc
    23401 attcgttgca tcagcgtacg gttttcaaca tagtttaata agtaaccgac attgagataa
    23461 ttgccgccag catctgcggt cgtggtgggt ttaggtgtgg gagtggggtt aggcgtagga
    23521 tcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc cggtgttggt
    23581 tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca gttagtgcca
    23641 tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc gctgaacgag
    23701 gccgcaccgt cagtggtttt taccactgtc agaacttcat ttcccgtagt ggcctcgctg
    23761 ccctggttgc ggatagccaa tacgtgatta ccagcactac ttccgctgat attcaataaa
    23821 tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat aataaaggta
    23881 ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt tgacccgtgg
    23941 ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga attacttgtt
    24001 acgttccaga cgctgttatt cattgcaacg tccagtttcc cgccattgac attatcgctg
    24061 agggaggaac ctgtccaaac cgaccctggg tgcatatcta gattgattag cccacctttg
    24121 gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct ggcggcataa
    24181 ccatcgtcat gttgcgttgc aatggccatc tggtcgggtg tactcatatc aatgaccaga
    24241 tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc tcctgccgcg
    24301 ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca caaacccagc
    24361 gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac aacagccgtt
    24421 gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg ttgcgctgcc
    24481 gtgccagaaa aattgattgt cgcgtctgaa ccactggtga cgaggccacc gccctgcgcg
    24541 gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc gacgccattg
    24601 gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc aaagctccag
    24661 aggccgtgtg cattatcgcc attggtttta atggaactgt ttgttccgag atcgacaaca
    24721 gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat tgtcaggtct
    24781 gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc gatataaaca
    24841 cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgacact ggtgccatag
    24901 tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt ggctgttagt
    24961 gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct gcctgtgcct
    25021 aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt ttgaccaaca
    25081 acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat aatgattcct
    25141 tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag gttgatattg
    25201 ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg atcggcggta
    25261 tctcctgtaa ttctgtcacc ttcattaata gtaatattgg tgccatattg acttcctgta
    25321 tcagcagcta tcagcagggg ggaaaagata attgcattca aaccaatagc gccaaatttt
    25381 atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc tttggtattc
    25441 ccattgtgtt gtttgatgcc cataccactc ctatatagta cccagactat gggtctatgg
    25501 attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga tatttgtatg
    25561 gtgcaatcat gatgagcttc tcagagaata attctctatt accgggcaaa atcaggaaca
    25621 caggaattaa tatatattta acatccccct tacaaaggtg agtttatatc gagttattcc
    25681 ctaagttgtc gttgcttatt ccaattcgtt actataaata tggcaaaaat attacaacaa
    25741 acagcggtta aaaaatttgg tgaagatttt ttgtaataat cctaattagt tatgggaatt
    25801 tcgcttgatg tatctgcata acaaaaacaa tatgcgccat ttttgcatta ctaataagaa
    25861 aaagcaaaaa acatcgatat tcatttttgg tcaatgggtt ttctggccta tcgttgttat
    25921 tgtgctactg gttatcattt tcatcttgcc aggatcgcac gtaatcacgt tattcgggga
    25981 ctttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat attggagcgt
    26041 gatcaatatt gatcagctgt gaagggggtg atgtgtagtg gaccaaaact tacgttatat
    26101 cactactaat gagatatccg tcatctgaca ctgatgtctt taattattct cgtggttcac
    26161 tgtaggcaat aagcacaaaa gtgtaggatg ttacaagaat gattaggact cggtgaaatg
    26221 aaaaatccac gcaattgcgt ggattatata tacttttgcg ctcttcatga gatttagcga
    26281 aacctcatga gacaacaaat taattagacg ttgaaaagga agttcatcac atcgccatct
    26341 ttaacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc gccttgttca
    26401 cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa gcctttttca
    26461 aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat
    26521 gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag cttataaccg
    26581 gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat aaactcgtca
    26641 cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac cggaaccaca
    26701 acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt gttttcaaaa
    26761 ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa
    26821 cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc tgcattttcc
    26881 aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt atcgccacct
    26941 ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc
    27001 agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga aacgtgaatg
    27061 atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc acggatgttg
    27121 gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag accggcgata
    27181 tcgacaaatt ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc agccagttga
    27241 tccaggcgag ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt gcagaatgga
    27301 aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga tttcccgacg
    27361 ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt aatatcttaa
    27421 taatccacct gttattgcta acagattgca gaaatggaaa taactttgcc tattatacac
    27481 ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg tgcaatcggt
    27541 tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc gcttcgtcga
    27601 tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca aaaccgacaa
    27661 ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag ttagggttat
    27721 tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca ccgcccaatt
    27781 taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg
    27841 gattaatgcg gaaaaaactg gccatcgcag caacggcttt gccgctgaga ttcataaatg
    27901 tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa gtataaccaa
    27961 agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc
    28021 aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc aggccgacaa
    28081 tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc gtagtttact
    28141 ggttgcggcc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta ataattgcat
    28201 gagtggacta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata
    28261 agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta tactttacac
    28321 ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga catatgaaac
    28381 gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg
    28441 tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc cagtatttcg
    28501 cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg ggtgcccgtg
    28561 ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat gacaaacgtt
    28621 gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct gaccagtcag
    28681 tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg acgattcggg
    28741 aagaacgcca ggcgtcccgg gatcggttga atgccagcgc gagccatagt gcgcagtggc
    28801 tggaattcca cgttgcacac gcgcagttca catccctcgg gcagacgctt cacaaaacgc
    28861 tggaacgcat caagaccacc agcatcaagt accggaaccg catcccactt cagaatcaca
    28921 atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc agcagcaaaa
    28981 aacagcgggc caataacgcg cagaaccagg acatcgtctg gaacatctac gactaccggt
    29041 gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag cacgatcccc
    29101 acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag cagcatgaca
    29161 atgatgtcat ctttcggcgc atgacgcagc aagtcgacca ctttgtgcgc ttcactcatg
    29221 ttccacgcca ccatcaacag cagggcagcc atggcggaaa gcggcagcca ggagagcagc
    29281 ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc cgagataggg
    29341 gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt agcggtaatg
    29401 ccaccaaaga acggagcgat gatattcccc agtccctgtc caaccagttc gctattcgcc
    29461 ttgtgtttcg tcccggtcat accatccagc accacggcgc agagcagaga ttcgattgcg
    29521 ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc
    29581 gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag ttgcggaata
    29641 ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca
    29701 tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa ggccggaagg
    29761 tgaccgggta aacgaatgcc cagacgcggc cagaaaacaa gaatacctag cgtcacaatg
    29821 ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc gccgactttt
    29881 tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt aatctgcatg
    29941 gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac cggaatatac
    30001 tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaaaatccc cgacagcaag
    30061 gtcgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagag aattaccaca
    30121 aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc aatgacaatc
    30181 cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc aatagccaac
    30241 gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag gtcacgggta
    30301 aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg gaaaggcatc
    30361 acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca tcatctgtca
    30421 actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga caaaaaaacc
    30481 cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag agatcgattc
    30541 ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac
    30601 gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc
    30661 aattacagag ttacgcaggt tattcgccgc gttgccagag aagatcgggt gagtcgcgta
    30721 cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc
    30781 gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat
    30841 atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt
    30901 atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac
    30961 cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa
    31021 tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt
    31081 cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg
    31141 tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc
    31201 ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag
    31261 gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt
    31321 ttcgttgatt tgtacactga cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag
    31381 tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa
    31441 aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg
    31501 ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc
    31561 aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc
    31621 tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc attccggggc
    31681 ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc
    31741 tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc
    31801 gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt
    31861 ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcaccggagt
    31921 cggaatactt acactagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt
    31981 tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc
    32041 tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag
    32101 atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc
    32161 catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct
    32221 gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat cttcatgttc
    32281 cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat
    32341 ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg
    32401 ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat
    32461 tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc
    32521 atattggctg gcatcgcagg ttgcgttttg gtgtcataac caccataaac aaccttccag
    32581 tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag
    32641 tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag cggaattggc
    32701 attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta
    32761 ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat
    32821 gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg
    32881 tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga
    32941 ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct
    33001 ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata
    33061 agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt gcagttataa
    33121 cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga
    33181 atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca
    33241 tcctgtatga tgcaagcaga ctaaccctat caacgttggt attatttccc gcagacatga
    33301 cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat
    33361 cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc
    33421 agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc
    33481 aggataacct gcaagaggcg ttaatcctct ggctttgcga ttatcacaat cttaatgaag
    33541 aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc
    33601 gtgttgccag cggcctagat tcactggttc ttggggagcc gcagatcctc ggtcaggtta
    33661 aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt
    33721 tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg
    33781 ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta
    33841 cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc
    33901 gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc
    33961 tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg
    34021 aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg
    34081 tggagcgcgc attaaaaagc cgtcgcaacc aaccaatgct gttggtggat attgccgttc
    34141 cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg
    34201 atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg
    34261 aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca
    34321 gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca
    34381 aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat
    34441 ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg
    34501 acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca
    34561 tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact
    34621 ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat
    34681 cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc
    34741 gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct
    34801 cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta aagaaaaaag
    34861 cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg
    34921 taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcagg
    34981 cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg tagaaatcat
    35041 gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga
    35101 tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc
    35161 tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact
    35221 gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc
    35281 gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt
    35341 gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa acaaagcgaa
    35401 ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac gccaacaggc
    35461 cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac
    35521 ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgcct
    35581 ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca
    35641 ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca ctggttacgt
    35701 gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg
    35761 gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac gcagctgact
    35821 gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt
    35881 gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc
    35941 ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa
    36001 caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct
    36061 agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg
    36121 gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg
    36181 tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac
    36241 gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt
    36301 gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg
    36361 gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa
    36421 gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag
    36481 cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc
    36541 ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga
    36601 agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt
    36661 tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg
    36721 cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg
    36781 cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt
    36841 tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag
    36901 tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc
    36961 tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc
    37021 gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagctgg
    37081 aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc
    37141 gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc agtgcggtat
    37201 cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg ctgccggtga
    37261 ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt tggctgatta
    37321 atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta aagggaaata
    37381 tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac attgaggtaa
    37441 tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag atggagctgg
    37501 cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat gaaattcgcg
    37561 atcgcgggtt gatttatgtg caactggatt gcgaacacgt tgcgttgaac gatttaagtt
    37621 atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg caaataaata
    37681 acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc aagattaagg
    37741 cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa atgacctgcc
    37801 gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg
    37861 cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca aagcctcttt
    37921 tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg aagaagggat
    37981 gaaaatcttc caggagttga agcagacttt tggcgtgaaa attatcaccg acgttcacga
    38041 accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc cggcgtttct
    38101 tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa
    38161 gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat tcaaagaagg
    38221 cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg acaacctggt
    38281 tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt
    38341 cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg
    38401 tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg ggctgtttat
    38461 tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc
    38521 taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga aaggtttcga
    38581 agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta gcgttctgcg
    38641 ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta cccctgcggc
    38701 cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc ttacttccgg
    38761 ttacgccacc agc
//
LOCUS       DAZUFB010000037        38575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZUFB010000037 DAZUFB010000000
VERSION     DAZUFB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.037"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHY3M_002128"
     CDS             <1..363
                     /locus_tag="ACHY3M_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4317044.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHY3M_002129"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHY3M_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFS4317045.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /gene="digH"
                     /locus_tag="ACHY3M_002130"
     CDS             2185..3504
                     /gene="digH"
                     /locus_tag="ACHY3M_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFS4317046.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHY3M_002131"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHY3M_002131"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFS4317047.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHY3M_002132"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHY3M_002132"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFS4317048.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRKNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVEIKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHY3M_002133"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHY3M_002133"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFS4317049.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYNAYRPQQAQAMLWQACPDPQYVVDVTVSSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /gene="ddpA"
                     /locus_tag="ACHY3M_002134"
     CDS             8540..10090
                     /gene="ddpA"
                     /locus_tag="ACHY3M_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4317050.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /gene="ddpB"
                     /locus_tag="ACHY3M_002135"
     CDS             10092..11114
                     /gene="ddpB"
                     /locus_tag="ACHY3M_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4317051.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHY3M_002136"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHY3M_002136"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFS4317052.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /gene="ddpD"
                     /locus_tag="ACHY3M_002137"
     CDS             12004..12990
                     /gene="ddpD"
                     /locus_tag="ACHY3M_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4317053.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /gene="ddpF"
                     /locus_tag="ACHY3M_002138"
     CDS             12983..13909
                     /gene="ddpF"
                     /locus_tag="ACHY3M_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4317054.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHY3M_002139"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHY3M_002139"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFS4317055.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14742..14957
                     /gene="bdm"
                     /locus_tag="ACHY3M_002140"
     CDS             14742..14957
                     /gene="bdm"
                     /locus_tag="ACHY3M_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFS4317056.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15059..15196
                     /gene="sra"
                     /locus_tag="ACHY3M_002141"
     CDS             15059..15196
                     /gene="sra"
                     /locus_tag="ACHY3M_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFS4317057.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15353..17050
                     /gene="maeA"
                     /locus_tag="ACHY3M_002142"
     CDS             15353..17050
                     /gene="maeA"
                     /locus_tag="ACHY3M_002142"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS4317058.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17184..18194
                     /gene="adhP"
                     /locus_tag="ACHY3M_002143"
     CDS             17184..18194
                     /gene="adhP"
                     /locus_tag="ACHY3M_002143"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFS4317059.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18341..18625
                     /gene="yddM"
                     /locus_tag="ACHY3M_002144"
     CDS             18341..18625
                     /gene="yddM"
                     /locus_tag="ACHY3M_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS4317060.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHY3M_002145"
     CDS             complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHY3M_002145"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFS4317061.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHY3M_002146"
     CDS             complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHY3M_002146"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFS4317062.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHY3M_002147"
     CDS             complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHY3M_002147"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22679..22681),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS4317063.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23498..24379
                     /gene="yddG"
                     /locus_tag="ACHY3M_002148"
     CDS             23498..24379
                     /gene="yddG"
                     /locus_tag="ACHY3M_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFS4317064.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            24638..24913
                     /gene="yddL"
                     /locus_tag="ACHY3M_002149"
                     /pseudo
     CDS             24638..24913
                     /gene="yddL"
                     /locus_tag="ACHY3M_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            24952..26283
                     /locus_tag="ACHY3M_002150"
     CDS             24952..26283
                     /locus_tag="ACHY3M_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFS4317065.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            26607..27995
                     /gene="narU"
                     /locus_tag="ACHY3M_002151"
     CDS             26607..27995
                     /gene="narU"
                     /locus_tag="ACHY3M_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFS4317066.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            28077..31817
                     /gene="narZ"
                     /locus_tag="ACHY3M_002152"
     CDS             28077..31817
                     /gene="narZ"
                     /locus_tag="ACHY3M_002152"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFS4317067.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLDNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            31814..33358
                     /gene="narH"
                     /locus_tag="ACHY3M_002153"
     CDS             31814..33358
                     /gene="narH"
                     /locus_tag="ACHY3M_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS4317068.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            33358..34053
                     /gene="narW"
                     /locus_tag="ACHY3M_002154"
     CDS             33358..34053
                     /gene="narW"
                     /locus_tag="ACHY3M_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS4317069.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            34050..34730
                     /gene="narI"
                     /locus_tag="ACHY3M_002155"
     CDS             34050..34730
                     /gene="narI"
                     /locus_tag="ACHY3M_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS4317070.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            34809..35702
                     /gene="yddE"
                     /locus_tag="ACHY3M_002156"
     CDS             34809..35702
                     /gene="yddE"
                     /locus_tag="ACHY3M_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFS4317071.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINVLHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(35798..36643)
                     /gene="nhoA"
                     /locus_tag="ACHY3M_002157"
     CDS             complement(35798..36643)
                     /gene="nhoA"
                     /locus_tag="ACHY3M_002157"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFS4317072.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            36816..37385
                     /gene="yddH"
                     /locus_tag="ACHY3M_002158"
     CDS             36816..37385
                     /gene="yddH"
                     /locus_tag="ACHY3M_002158"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4317073.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(37389..37616)
                     /gene="pptA"
                     /locus_tag="ACHY3M_002159"
     CDS             complement(37389..37616)
                     /gene="pptA"
                     /locus_tag="ACHY3M_002159"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFS4317074.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(37715..>38575)
                     /locus_tag="ACHY3M_002160"
     CDS             complement(37715..>38575)
                     /locus_tag="ACHY3M_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFS4317075.1"
                     /translation="WMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMH
                     SLVLRQIKTDEKSNEITAIPELLNMMDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFA
                     VKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTF
                     EWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLH
                     WRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYL
                     ASVLAGRRLS"
BASE COUNT         9534 a         9725 c         9634 g         9682 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggttattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagtgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa gaacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaat
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacaatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcagtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgattg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgc
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaatttcc
    14521 acaatagata accgggaata tataacccaa gagatattgc attaatatta atcaactcac
    14581 cacaataata atacctatca atatcatgag gttaaaatat aaaaatcatc aaccaggact
    14641 aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat
    14701 ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact tattatcagg
    14761 cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag
    14821 agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt
    14881 ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc
    14941 agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg gtttagcacg
    15001 tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat
    15061 gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt ctaaccagcg
    15121 caaaatagtt accgaaggtg acaaatccag cgtggtaaat aatccaaccg gcagaaaacg
    15181 ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg
    15241 cgcaatggtg tttttgtttt tatctgctct atacttgggg ccgacgccct ggcggtaaag
    15301 caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg acatggaacc
    15361 aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt
    15421 tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct
    15481 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
    15541 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
    15601 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
    15661 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
    15721 cggcgtgttt atctcttacc agaaccggca caatatggac gatattctgc aaaacgtgcc
    15781 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
    15841 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
    15901 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
    15961 gctgcttaac gatccgctgt atatgggctg gcgtaatcca cgtatcactg acgatgaata
    16021 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
    16081 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
    16141 aatttgttct tttaacgatg acattcaggg cactgcggca gtaacagtcg gcacactgat
    16201 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
    16261 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
    16321 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
    16381 caagatgccg aacctgctac ctttccagac caaactggtg cagaagcgcg aaaacctcag
    16441 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
    16501 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcattcgtga
    16561 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtcacgcgt
    16621 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
    16681 cagcccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
    16741 cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac
    16801 cgatgagatg ctgatgtcag caagtgaaac gcttgctcag tattcgccgc tggtgctgaa
    16861 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
    16921 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc
    16981 tctgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
    17041 ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
    17101 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
    17161 acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg
    17221 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
    17281 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaactg
    17341 gggtaattct gggccatgaa ggcatcggtg tggtggcaga agtgggtcca ggtgtcacct
    17401 cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg
    17461 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
    17521 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
    17581 atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag
    17641 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
    17701 tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg atcgccattg
    17761 atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact
    17821 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
    17881 tggtaacagc agttgctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
    17941 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgccttg
    18001 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
    18061 ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg
    18121 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
    18181 atttccgccg ttaagaggcc tttgctgcga gtgccagtcc tggtgtcgca gcatcgcact
    18241 ctctccgaca ttaaatggct aaatggatta ttgttccttc ctacgcaaca ataatgatag
    18301 tgttcctgat gaaggaacac tactgagagt aaagacactg atgaaaatgg ccaatcatcc
    18361 ccgcccgggg gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt
    18421 tgccagagca atggaaattg ctccctcaac ggcaagccga ttgctgaccg gaaaagcagc
    18481 tttgacacca gaaatggcga ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg
    18541 gctgaatctg caaaacgcct ggagtctggc agaggcagaa aaaacggtgg atgtgtcgcg
    18601 actacgccgt ctggttacgc aataaaaaaa tggcgcttct taaagcgcca ttttcagctt
    18661 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    18721 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cggctcacct tcccttcgat
    18781 catcccttta atcgaccctt tcacccagaa tgccatatac atatggatca ggatggcgtg
    18841 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    18901 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    18961 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19021 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19081 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19141 cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19201 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19261 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19321 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19381 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19441 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19501 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    19561 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    19621 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    19681 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    19741 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    19801 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    19861 cagcagacgg gcacgccttc aggcagccgg gatcttcaca gtgcatacag ccgtctttac
    19921 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    19981 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20041 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acagccgata caggtggaaa
    20101 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20161 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20221 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20281 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20341 agtggattgg aatacccacc gtttcaacct gctgaccatt tacattcagt ggtttcagac
    20401 gacgcgttac cacagccacc gcgcggataa agccacgctt gcttgagaca gtgacacgat
    20461 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    20521 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    20581 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagt gcgtcttgtt
    20641 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    20701 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    20761 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    20821 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    20881 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    20941 gacggttgag cggccacgcc caggcccatc ccagcgtatt ccccagaccg gacgggtcgg
    21001 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21061 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21121 tcttcgcaat cagcacgcca ttagcatcat agagatcttc cagcgcatag ccgttgttct
    21181 ctttagctac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21241 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcagg tgatggtaga
    21301 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcatcctga cctttccagt
    21361 gccactgcag ccagcgaccg gagttagcaa tagaaccatc ttcttcagca aagcaggtcg
    21421 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcgccgt
    21481 ggttctgcca gaaggttgag gtttcagtca ccagcggatc gataaccacc atgtacttca
    21541 gcttgcttag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    21601 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    21661 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    21721 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    21781 tcacctgacc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    21841 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc agacctaagt
    21901 cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt cacgccgcca ccggccatac
    21961 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacgg
    22021 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22081 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccacag atgttttcaa
    22141 caacgtccgg cgtgtagcgg gaaacgtgcg ctttcagcag gttccacaca cagcgcggat
    22201 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22261 atttatcgta ttgccgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22321 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22381 tgtcgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22441 gaataggtgc gtaaatatcc gccacagaag cggtacgcgt aaaacgagga tcgacaacga
    22501 tcaaggttgc atcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    22561 cttcagcagc gttaccgccc attaccatta cgacgttagc gtttttgata tccacccagt
    22621 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    22681 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgtgtca
    22741 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    22801 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    22861 gctttgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    22921 gcgcacgata ttccgggtag cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg
    22981 ccccttttgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23041 aaatcgcttc tctggcgttt ttcgcgccat cacccaggct atacatcaat agcccgcaac
    23101 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23161 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt gttccagcca
    23221 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23281 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23341 tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac ctacctcaat
    23401 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23461 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    23521 catagggcta atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    23581 cgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    23641 aatcttcacg gttggatttc cgcgtattcg gcagattccg aaaggctatt tactcgccgg
    23701 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    23761 ccgtcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    23821 ctttgccatt ctgtttaatg gtcagaaaac caactggctg attgtaccag gattattatt
    23881 agccctggtc ggcgtctgct gggtgttagg cggtgagaat gggttacatt atgatgaaat
    23941 catcaataat atcaccacca gcccattgag ttatttcctg gcgtttattg gtgcgtttat
    24001 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaacg gaattaccgt
    24061 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttctcacgc cacaaccaga
    24121 aatggtattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24181 tgcttatgct gcatggaatg tcggtatact gcatggcaat gtcaccataa tggcggtagg
    24241 ctcgtatttt acgcccgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24301 gtcgttctca ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct
    24361 ggcgacacgt cgtggttaaa ataattgccg ggtctacggc ccggcagtta atcagataaa
    24421 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
    24481 acttcgatca catatcgata aataatatta tttcatctat ttttctcatt gtttttaatc
    24541 gccattctat tttaaaaaca gtatatcatt tataaatgac gccgtttaaa attcgtttag
    24601 aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt
    24661 ggttgtaact ggtttgttag ctgcgaacgt agcgcacgct gccgaagtcg ataacaagga
    24721 tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa
    24781 gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa
    24841 tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc
    24901 caacggctcg cgcggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact
    24961 gaccttatat tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt
    25021 gcacatttaa acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat
    25081 ttactgccaa ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta
    25141 gttaacgtca tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat
    25201 ataattgatg acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa
    25261 ccaccagggc tgcaaaactt cacctctatc aatcttgata ataaccaact cacacatttt
    25321 gatgcaacca actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca
    25381 ataaattttc ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct
    25441 ctcagaaata ttgatataga taggctttca tcagttactt attttagtgc ggcacataat
    25501 caactagagt ttgtgcaatt agaatcttgc gaatggctgc aatacctgaa tctcagccat
    25561 aatcaattaa ctgatattgt tgcaggtaat aaaaatgaac tcttactgct ggatctatcc
    25621 cataataaac taacaagttt acacaatgac ttatttccca acttgaatac gttacttatt
    25681 aacaacaatt tgctttctga aattaaaata ttctatagca acttctgcaa tgttcagaca
    25741 ttaaacgctg ctaacaacca gttgaaatat ataaatcttg atttcctgac ttatcttcca
    25801 tctatcaaaa gtttaagact ggacaataat aaaataaccc acattgatac taataataca
    25861 tccgatattg gaactttatt ccccataata aaacagagca aaaacttaaa ttttttaaat
    25921 gtttctggga agaacaattg ccctactatg cagctcatgt tatttaattt gttttcccca
    25981 gcacttaagc ttaatactgg cctggcaatt ctttcgcctg gtgcatttga agttcactct
    26041 gacggaatag atgtggataa cgaattgttt cactatccta ttaaaaaagc atatacccca
    26101 tataatatac acacttacaa gacagaggaa gttgtaaacc agaggaatat aaaagttaaa
    26161 aacatgacct tagatgaaat aaacaatact tactgtaata acgattatta caatcaggca
    26221 ataagagagg aaccgataga ccttctggac agatcgtttt cctccagttc atggcctttt
    26281 tagtgaataa atcatcacta attctataag tgtctggtct tgttgataat atagttaata
    26341 tgtaaccctt tagattcacg atctgaaggg ttgaaacatg acatacgttc aaagattaac
    26401 ccccgtttga aggttagctt gttagttaca tttagtaaca catatctggc tacttaatcc
    26461 ccattctgcc cccgcggtta cgctttctta tctattcttt caatcagcac ttcacccaat
    26521 tcatccttaa ttctaataac tggctattgc gccttacctc ctgttttgtc ttttttcatt
    26581 accaatgtgt gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat agtcgttatc
    26641 ttttacgcga ctggaaacca gaaaatccgg ccttctggga aaataaagga aaacatattg
    26701 ctcgaagaaa cctctggata tcagtcagtt gtctacttct tgccttctgt gtctggatgc
    26761 tatttagcgc agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac
    26821 tctttttatt aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct
    26881 ttatggtgcc tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta
    26941 ttccttgcgt ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta
    27001 tcgttatcgc tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata
    27061 tcagtttctt ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag
    27121 gaaacttagg tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat
    27181 ttgcctttct cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga
    27241 atgccgcatg gatttgggta ccgctactgg cgattgccac gatcgccgca tggtcaggga
    27301 tgaatgatat cgccagttca cgcgcctcaa ttgccgacca gctccctgtc ttacaacgcc
    27361 tgcatctctg gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt
    27421 ctgcgggttt tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg
    27481 cgttctttgg cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata
    27541 agttcggcgg cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc
    27601 tgctgttcct taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat
    27661 ttatggggct gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg
    27721 tcatctttcg ccagataacc atttatcggg taaagatgaa aggcggtagt gatgagcaag
    27781 ctcagaaaga agccgtcacc gaaacggcgg cggctctggg ctttatctca gccattggcg
    27841 cagtgggcgg cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc
    27901 cggtcggcgc gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc
    27961 tggtttatgg tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg
    28021 tgtcgcatcc gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga
    28081 gtaaactttt ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc
    28141 acggacaggt gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt
    28201 tcgacaaaat cgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa
    28261 tctacgttaa aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc
    28321 gccctgacct gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt
    28381 atctttacag cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt
    28441 ggcgcgaagc cctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg
    28501 atccgcaaaa gtgcctgagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca
    28561 actggcagga actaaaccag ctgattgccg ccgctaacgt ctggaccatc aaaacctacg
    28621 gcccggatcg cgttgccggt ttctcgccga tcccggcgat gtcgatggtt tcttacgccg
    28681 ccggaacgcg ttatctgtcg ctgcttggcg gcacctgttt aagtttctac gactggtatt
    28741 gcgacctgcc gcccgcctcg ccgatgacct ggggcgagca aaccgacgta ccggaatctg
    28801 ccgactggta taactccagc tacatcatcg cctgggggtc taacgtaccg cagacacgta
    28861 cgccggacgc ccacttcttt accgaagtac gctacaaagg cactaaaacc atcgccatta
    28921 cccctgacta ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca
    28981 ctgatagcgc cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata
    29041 atcccagcga ctactttatc aactactgcc gccgctacag cgacatgccg atgctggtaa
    29101 tgctggagcc tcgcgacgat ggtagctacg ttcccgggcg catgatccgc gcatctgacc
    29161 tggtggatgg actgggcgaa agcaacaatc cgcagtggaa aaccgtagca gttaataccg
    29221 caggtgaatt ggtagtgccg aacggttcga ttggtttccg ctggggagaa aaaggcaaat
    29281 ggaatctgga atccattgcc gccggtacgg aaaccgaatt gtcgttaacc ctgctcggtc
    29341 aacatgacgc tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt
    29401 ttcgcagcgt aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt
    29461 tagtcgatgg caacacctgt ccggtggtca gcgtttatga tttggtactg gcgaattacg
    29521 gcctcgatcg cgggctggaa gatgaaaaca gtgcgaaaga ttacgctgaa atcaaaccgt
    29581 acaccccagc ctggggtgag caaattaccg gcgtgccgcg ccagtatatt gaaaccatcg
    29641 cccgtgaatt tgccgatacc gcccataaaa cgcatgggcg ctcgatgatt atcctcggcg
    29701 caggtgttaa ccactggtat cacatggaca tgaactaccg cgggatgatc aatatgctga
    29761 tcttctgcgg ttgtgtcggg caaagcggtg gcggctgggc acactatgtc ggccaggaaa
    29821 aactgcgccc acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgaccac
    29881 cacgccagat gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa
    29941 aagtctctgc tcaggagtta ctttcaccgc tcgccgatgc cagtaagtac agcggtcatc
    30001 tgattgattt caacgttcgc gccgaacgta tgggctggct accttctgcg ccgcagctgg
    30061 ggcgtaaccc gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat
    30121 ttaccgccca ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg
    30181 gcagcaatca tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca
    30241 aaggccacga gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg
    30301 aactcggtgc cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg
    30361 aaggcaagct cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct
    30421 ccgatatcgt tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata
    30481 tgcatccgtt tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg
    30541 actgggaaat ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg
    30601 gcaaagaaac cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac
    30661 agccgtgtga agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg
    30721 cgccgaatat tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc
    30781 tcgggccatt gatggacaaa cttgacaatg gcggtaaagg gatttcgtgg aatacgcagg
    30841 atgaaatcga tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc
    30901 gtccgctgat tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa
    30961 ccaacggtca tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac
    31021 atacccatct ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc
    31081 agccgcgtaa aattatctcc agccccacct ggtccggtct ggaaagcgat cacgtatcct
    31141 ataacgcggg atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc
    31201 agcagctcta tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc
    31261 gcccgcctat cgacacccgt agcgtcagtg agatgcgcca gataccgcca aacggcttcc
    31321 cggaaaaagc acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca
    31381 gtgaaaacct gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa
    31441 cagatgcccg agaactgacc attgtcgata acgactgggt ggaagtattc aacgccaacg
    31501 gcgcgctgac tgcccgcgcg gtggtcagcc aacgtgtacc gccgggcatg accatgatgt
    31561 atcacgccca ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg
    31621 gcattcataa ctcggtcacc cgcgtttgcc cgaaaccaac gcatatgatt ggcggttacg
    31681 cgcagctggc ctggggcttt aactactacg gcaccgtcgg atcgaaccgc gatgagttca
    31741 tcatgatccg caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac
    31801 aggaggcgaa aaaatgaaaa tccgttcaca agtcggcatg gtgcttaacc tcgacaaatg
    31861 tatcggctgc catacctgtt cggtgacctg taaaaacgtc tggaccgggc gcgaaggcat
    31921 ggagtacgca tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg
    31981 ggaagatcag gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatccgtcc
    32041 gcgtctgggc agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca
    32101 gattgatgat tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga
    32161 aggcaaacat atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa
    32221 agtgatctgg ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg
    32281 cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat
    32341 gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc
    32401 aagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg
    32461 ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa
    32521 aagcggcaag tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac
    32581 cgtgtgctca gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc
    32641 cgaccgcatt gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg
    32701 cgaagtattc ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat
    32761 tccacaaaac gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg
    32821 gaaactagcg ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc
    32881 tccgctgtcg ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt
    32941 gctgcccgcc atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc
    33001 cggcgatacc ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat
    33061 gcgttcacaa accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag
    33121 cgtcgcccag gtcgaagaga tgtatcgtta cctcgccatt gccaactatg aagatcgttt
    33181 tgtcatcccg acgagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg
    33241 cggttttacc tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag
    33301 tagccgtatc gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg
    33361 cagatcctca aagtgatcgg cctgttgatg gagtatccgg acgagctgtt gtgggaatgc
    33421 aaggaggacg cgctggcgtt gatccgcagc gacgcgccga tgcttacgga tttcacccac
    33481 aacctgctta acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgt
    33541 ggacgtacca catcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc
    33601 caggcaatgg tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg
    33661 gaactgcccg attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag
    33721 gcgaaagaag gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa
    33781 caacgcgagg caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagctcc
    33841 ctttcaagtg acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag
    33901 gcgcttgatg cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg
    33961 tgtgacagct cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcaa
    34021 tatgtcgaca tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg
    34081 atatctaccc gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact
    34141 acgggcagta cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga
    34201 tatggtcgaa tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca
    34261 tgttaacgcc gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactaatgg
    34321 cgatggtgct cggcggtatt tgcggtgtgt tgacattgat tggcggcgca ggactgctgt
    34381 ggcgcaggct gacaaatcag cgcgtgcgtg caacttccac tacgccggat attatcatca
    34441 tgagcatttt gctgatccag tgtctgctgg ggctaagtac cataccgttt tcagcacagt
    34501 atcctgatgg tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc
    34561 gtggtggatc gtcagaaatg cttaacggcg tagcgttcgt tttccgcctg catctggtat
    34621 tgggaatgac catcttcctg ctcttcccgt tcaccagatt ggtgcatgtg tggagcgcgc
    34681 cgtttgagta ctttactcgt cggtatcaga ttgtgcgttc gcggcgataa tttcaccctg
    34741 gccggatagc ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga
    34801 gaacgttaat gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg
    34861 gcaattctgc cggtgttgtt ttccccgccg ataatcttag cgaagcgcaa atgcagctta
    34921 tcgcccgcga gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg
    34981 tgcgcatccg ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag
    35041 ctgcgcacta tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat
    35101 cgctggcagg aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc
    35161 tggaacaagg tacgccgggc tttgagccac cgctggaagg tgaaacacgt gaggcgatta
    35221 tcaacgtgct tcatctcact gaagacgata ttttgccggg cttgccaatt caggtcgcaa
    35281 caacaggaca ctcaaaagtg atgatcccgt tgaaaccgga agtggatatc gacgcccttt
    35341 cgcctgatct tgctgcgctg agcgctatca gcaaacagat tggctgcaat ggtttcttcc
    35401 cgttccagat ccgcccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg
    35461 gtattgtgga agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac
    35521 atcacaacgt attgccccac gatggcaacg tgttatgtgt taaaggccat caggggcgcg
    35581 cattggggcg tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa
    35641 aagtcaccat ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaactct
    35701 gaaaccgttg cctgatgcgc ttcgcctgtc aggtctacaa ccgagctaat gtatgccgga
    35761 taaggcgttt acgccgcatc cggcaacctg acataaatta ttttcccgcc tccgggtgcg
    35821 tatcaaacgc cgccatcacc agcgctaact catccacggt aaagccatgt ttcgcatcat
    35881 ccacgcccag accaaactgt tcttgcatca cagcatataa tgacgccaca tccggtagat
    35941 ttcgctgctc caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagcgtta
    36001 gcttgccacc gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg
    36061 gccagtgcgc cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat
    36121 caaaacggta catcgactgc cagtgctgat gatgattaaa ctgcaacacc caatcatcac
    36181 cctcctgcaa caaccgataa tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac
    36241 gaatcggcgc ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcctctt
    36301 ccagttccac caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca
    36361 ctacgcgccc taacaagctg cgaacgttaa actccagctc gcgtaacacc cgctcaaaca
    36421 cgccattctg ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg
    36481 attgatcatc aagctgtatt tccctcggca gcaaaacgtc gaggttttca aacggaatag
    36541 tgcaattgtg tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg
    36601 accagttaat acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt
    36661 ggttttctca acttataacc tggaatagac gccgcaccgg ctattttgtg tgatcgcgga
    36721 catgcatcac gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac
    36781 tacgcttaaa aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat
    36841 tacaccatgc cagccgtctg ttaaatcatg gcccaactgt catgatcacc agtttcgatg
    36901 agcaatccca gcggcgtaac attatggcag cagcctggtc aatgccggtg gagtttgaac
    36961 caccacgcgt ggcgattgtg gtagataaat cgacatggac cagggagttg attgaacgta
    37021 acggtaaatt tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcagtgg
    37081 gaagcgtgtc gggtcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag
    37141 gtccggtact tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc
    37201 tgctacctgc gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag
    37261 cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca
    37321 atacgttgca tcatttaggt gctggcacgt ttgttaccag cggcaagcga gttacggcgg
    37381 gttaagcatt acatgctgta accgggtttc tttatcaaag cctccatttg gggcgcgatt
    37441 tcggcatccc agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc
    37501 gagctatctt tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct
    37561 gctttttgtt gttcgtccag ttcacgcgga aaacatttaa tgtcgatgtg cggcatgatt
    37621 gtgctccttt tgaaagatgt cagtaaaatt aacacacatt gtgaatgtcg tgtgtccggt
    37681 aatgcgtttt tacgacaata atgccagggc aagattacga aagccttctc cccgcaagga
    37741 ctgacgccag gtagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct
    37801 tgaatacctt atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt
    37861 ctgctgcatt tccttttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc
    37921 agtgcagctt attctccacg tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg
    37981 ttaaatcagc agaactgata taatatctga ccgtcatttc tggctctttc ttttgttctg
    38041 ctattatgga ccgaaaggag actgccacgc ataatttctt cagccctttc cattcaaacg
    38101 tgaaatcaat aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc
    38161 cgtgactctt ttcactcatt gcgtaactgt catgctctgg attatttaat tctttcagcg
    38221 gaaatttttc ctcaaaggct ttatttagcc gcccctggtt tccttttacc gcgaataaat
    38281 aatcacctcc ctgtttttgt atcttttctg caatatcttt ctggcagccc atcgcatcag
    38341 ttgtgatgat ttttccttta atatccatca tgttaagaag ttcaggaatg gctgtaatct
    38401 cattggattt ctcatccgtc ttgatctgtc tgaggaccag actatgcatt gttgagaacg
    38461 cactaatgac atgaatcgct cccctgcgac gactcttgtc ataagagtgc cggagcgttt
    38521 ttccatcaat tgcaatgacg tctttatcat ctgaagaatg gcagtcacgc atcca
//
LOCUS       DAZUFB010000038        37718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZUFB010000038 DAZUFB010000000
VERSION     DAZUFB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.038"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            113..1801
                     /locus_tag="ACHY3M_002161"
     CDS             113..1801
                     /locus_tag="ACHY3M_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFS4317076.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            1798..2712
                     /locus_tag="ACHY3M_002162"
     CDS             1798..2712
                     /locus_tag="ACHY3M_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4317077.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2744..2992
                     /locus_tag="ACHY3M_002163"
     CDS             2744..2992
                     /locus_tag="ACHY3M_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4317078.1"
                     /translation="MVNREIVMDYILFCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2992..4164
                     /locus_tag="ACHY3M_002164"
     CDS             2992..4164
                     /locus_tag="ACHY3M_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4317079.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEVIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4200..5279)
                     /gene="lptG"
                     /locus_tag="ACHY3M_002165"
     CDS             complement(4200..5279)
                     /gene="lptG"
                     /locus_tag="ACHY3M_002165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS4317080.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5276..6346)
                     /gene="lptF"
                     /locus_tag="ACHY3M_002166"
     CDS             complement(5276..6346)
                     /gene="lptF"
                     /locus_tag="ACHY3M_002166"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS4317081.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6377..6937)
                     /locus_tag="ACHY3M_002167"
     CDS             complement(6377..6937)
                     /locus_tag="ACHY3M_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS4317082.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6949..7773)
                     /locus_tag="ACHY3M_002168"
     CDS             complement(6949..7773)
                     /locus_tag="ACHY3M_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317083.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            complement(7773..9122)
                     /locus_tag="ACHY3M_002169"
     CDS             complement(7773..9122)
                     /locus_tag="ACHY3M_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4317084.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9168..9926)
                     /locus_tag="ACHY3M_002170"
     CDS             complement(9168..9926)
                     /locus_tag="ACHY3M_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317085.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9958..10671)
                     /gene="yghS"
                     /locus_tag="ACHY3M_002171"
     CDS             complement(9958..10671)
                     /gene="yghS"
                     /locus_tag="ACHY3M_002171"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4317086.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            10845..11537
                     /gene="yghT"
                     /locus_tag="ACHY3M_002172"
     CDS             10845..11537
                     /gene="yghT"
                     /locus_tag="ACHY3M_002172"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4317087.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            complement(11586..13084)
                     /gene="pitB"
                     /locus_tag="ACHY3M_002173"
                     /pseudo
     CDS             complement(11586..13084)
                     /gene="pitB"
                     /locus_tag="ACHY3M_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(13376..15235)
                     /gene="gss"
                     /locus_tag="ACHY3M_002174"
     CDS             complement(13376..15235)
                     /gene="gss"
                     /locus_tag="ACHY3M_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFS4317088.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15440..16306
                     /gene="yghU"
                     /locus_tag="ACHY3M_002175"
     CDS             15440..16306
                     /gene="yghU"
                     /locus_tag="ACHY3M_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFS4317089.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16430..16678)
                     /gene="hybG"
                     /locus_tag="ACHY3M_002176"
     CDS             complement(16430..16678)
                     /gene="hybG"
                     /locus_tag="ACHY3M_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFS4317090.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16691..17032)
                     /gene="hypA"
                     /locus_tag="ACHY3M_002177"
     CDS             complement(16691..17032)
                     /gene="hypA"
                     /locus_tag="ACHY3M_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS4317091.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17025..17513)
                     /gene="hybE"
                     /locus_tag="ACHY3M_002178"
     CDS             complement(17025..17513)
                     /gene="hybE"
                     /locus_tag="ACHY3M_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFS4317092.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17506..18000)
                     /gene="hybD"
                     /locus_tag="ACHY3M_002179"
     CDS             complement(17506..18000)
                     /gene="hybD"
                     /locus_tag="ACHY3M_002179"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFS4317093.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18000..19703)
                     /gene="hybC"
                     /locus_tag="ACHY3M_002180"
     CDS             complement(18000..19703)
                     /gene="hybC"
                     /locus_tag="ACHY3M_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFS4317094.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19700..20878)
                     /gene="hybB"
                     /locus_tag="ACHY3M_002181"
     CDS             complement(19700..20878)
                     /gene="hybB"
                     /locus_tag="ACHY3M_002181"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFS4317095.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20868..21854)
                     /gene="hybA"
                     /locus_tag="ACHY3M_002182"
     CDS             complement(20868..21854)
                     /gene="hybA"
                     /locus_tag="ACHY3M_002182"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFS4317096.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21857..22975)
                     /gene="hybO"
                     /locus_tag="ACHY3M_002183"
     CDS             complement(21857..22975)
                     /gene="hybO"
                     /locus_tag="ACHY3M_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFS4317097.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSGKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23164..23451)
                     /gene="yghW"
                     /locus_tag="ACHY3M_002184"
     CDS             complement(23164..23451)
                     /gene="yghW"
                     /locus_tag="ACHY3M_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFS4317098.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23570..24457)
                     /gene="yghX"
                     /locus_tag="ACHY3M_002185"
     CDS             complement(23570..24457)
                     /gene="yghX"
                     /locus_tag="ACHY3M_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFS4317099.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24614..25654
                     /gene="gpr"
                     /locus_tag="ACHY3M_002186"
     CDS             24614..25654
                     /gene="gpr"
                     /locus_tag="ACHY3M_002186"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFS4317100.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25694..26188)
                     /gene="yqhA"
                     /locus_tag="ACHY3M_002187"
     CDS             complement(25694..26188)
                     /gene="yqhA"
                     /locus_tag="ACHY3M_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFS4317101.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26379..27263
                     /gene="yghA"
                     /locus_tag="ACHY3M_002188"
     CDS             26379..27263
                     /gene="yghA"
                     /locus_tag="ACHY3M_002188"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS4317102.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27535..27960)
                     /gene="exbD"
                     /locus_tag="ACHY3M_002189"
     CDS             complement(27535..27960)
                     /gene="exbD"
                     /locus_tag="ACHY3M_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFS4317103.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27967..28701)
                     /gene="exbB"
                     /locus_tag="ACHY3M_002190"
     CDS             complement(27967..28701)
                     /gene="exbB"
                     /locus_tag="ACHY3M_002190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS4317104.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28953..30140
                     /gene="metC"
                     /locus_tag="ACHY3M_002191"
     CDS             28953..30140
                     /gene="metC"
                     /locus_tag="ACHY3M_002191"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFS4317105.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30280..30939
                     /gene="yghB"
                     /locus_tag="ACHY3M_002192"
     CDS             30280..30939
                     /gene="yghB"
                     /locus_tag="ACHY3M_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFS4317106.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30979..31878)
                     /gene="yqhC"
                     /locus_tag="ACHY3M_002193"
     CDS             complement(30979..31878)
                     /gene="yqhC"
                     /locus_tag="ACHY3M_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFS4317107.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32072..33235
                     /gene="yqhD"
                     /locus_tag="ACHY3M_002194"
     CDS             32072..33235
                     /gene="yqhD"
                     /locus_tag="ACHY3M_002194"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4317108.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33340..34167
                     /gene="dkgA"
                     /locus_tag="ACHY3M_002195"
     CDS             33340..34167
                     /gene="dkgA"
                     /locus_tag="ACHY3M_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFS4317109.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34367..35293
                     /gene="yqhG"
                     /locus_tag="ACHY3M_002196"
     CDS             34367..35293
                     /gene="yqhG"
                     /locus_tag="ACHY3M_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFS4317110.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35344..35601
                     /gene="yqhH"
                     /locus_tag="ACHY3M_002197"
     CDS             35344..35601
                     /gene="yqhH"
                     /locus_tag="ACHY3M_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFS4317111.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35644..36240)
                     /locus_tag="ACHY3M_002198"
                     /pseudo
     CDS             complement(35644..36240)
                     /locus_tag="ACHY3M_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
     gene            complement(36488..37018)
                     /locus_tag="ACHY3M_002199"
     CDS             complement(36488..37018)
                     /locus_tag="ACHY3M_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317112.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            37209..37457
                     /locus_tag="ACHY3M_002200"
     CDS             37209..37457
                     /locus_tag="ACHY3M_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4317113.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            37459..>37718
                     /locus_tag="ACHY3M_002201"
     CDS             37459..>37718
                     /locus_tag="ACHY3M_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4317114.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKVDESPAPVVTKARRS"
BASE COUNT         9497 a         9879 c         9313 g         9029 t
ORIGIN      
        1 ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat ttttgatatc
       61 caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac ctatgcgcta
      121 tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta gcgccggaat
      181 gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga cattaaaaac
      241 gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag gcgaacgcgt
      301 ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct gtcagtatgc
      361 tggcttagtc gccgtcccgt tggcgattcc aatgggcgtc ggtcagcggg attcctggag
      421 cgccaaactc cagggtttac tggcaagttg ccagcccgca gccattatca ctggtgatga
      481 gtggttgcca ctggtcaatg ccgcgacgca taacaacccc gaattacatg ttttaagcca
      541 cgcctggttt aaggcattac cggaagccga tgttgcgctc cagcgtccag ttccgaacga
      601 tatcgcctac ctccagtaca cctccggcag cacccgtttt ccccgtggcg tcattatcac
      661 ccatcgcgaa gtgatggcta atctacgtgc tataagccac gacggcatta aattacgccc
      721 tggcgaccgc tgcgtctcct ggctgccttt ctaccatgat atgggactgg tcggctttct
      781 cctgaccccc gtcgccacgc agctttcagt agattatttg cgcactcagg attttgccat
      841 gcgtcctctg caatggctta aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc
      901 gccgtttggc tatgaattgt gccagcgccg cgtgaatgaa aaagatctcg ctgaactgga
      961 tctttcctgc tggcgcgtcg ctggtattgg tgcagaaccc atctccgcag aacaactcca
     1021 tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat aaaactttca tgccgtgcta
     1081 cggactggca gaaaatgcgc tggctgtcag cttctctgat gaagcctccg gggttgtggt
     1141 taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc cgggtgcaga
     1201 gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac atggcattga
     1261 aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata tttgcatctc
     1321 cggccccagc ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg agattgccgc
     1381 gacgggctgg ttagacaccg gcgacctcgg ttatctgctg gacggttatc tgtatgtcac
     1441 cggacgcatt aaagatctga ttattattcg tggccgtaat atctggccgc aggatattga
     1501 atatattgcg gaacaagaac cggaaattca ttctggcgat gcgattgctt ttgttaccgc
     1561 ccaggaaaaa atcattttgc agatccagtg tcggatcagc gacgaagaac gtcgcgggca
     1621 gcttatccac gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg cggctatcga
     1681 tctgttgccg ccccacagta ttccccgaac gtcctccggc aagcctgccc gtgcggaagc
     1741 gaaaaaacgt tatcagaagg cttatgctgc cagtcttaat gtgcaggaat ccctggcatg
     1801 aatcaaactg tcgcggtgac gggcgctacc gggtttatcg gtaaatatat tatcgataac
     1861 ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat
     1921 gataatctca cctgggtgcg cggttcgctg gaagatactc attcacttag caagctggtt
     1981 gccggagcca gcgcagtggt ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt
     2041 ttcacccgct gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa agagagctgc
     2101 ttttgccaac gttttctgtt tatctcttcg ctggcggcgc gccatcccga gctctcctgg
     2161 tacgcaaatt ccaaacacat cgccgaacaa cggctgactg caatggctga cgaaattacg
     2221 ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg
     2281 tttgactgga tgctgcgcgg cctgctgcca cgacttggtg caccagatac acagctctct
     2341 ttcctgcacg tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta
     2401 cagacgcaaa cctatgaatt atgcgatagc gtcgctggcg gctatgactg gcaacgcata
     2461 cagcaacttg ccgccaacgt ccgttgcggt tccgtgcgaa tggttggtat tcctctgccg
     2521 gtactcacct gccttgcgga tatcagtacc gcgttgagtc gcctggcagg taaagaacct
     2581 atgctgaccc gctcgaaaat tcgtgaatta acccacgccg actggtcggc aagtaataac
     2641 cgtatttctg aagatattaa ttggtttccc gggattagcc tggaacacgc attacgcaac
     2701 gggctatttt gattgtcttg tttaaaggtg tgttaacgtt tctatggtaa atcgtgaaat
     2761 agtaatggat tacatccttt tctgtttaca ggatttggtc gaaaacggtg tggaaattaa
     2821 gcccgacagc gatctcgtca acgatcttgg cctggagtcg atcaaagtga tggatctctt
     2881 aatgatgctg gaagacagat tcgatatttc cattcctatt aatattctgc tggatgtcaa
     2941 aacccctgcg caattaatgg aaactctact tccatggctg gagaataaat aatggggcta
     3001 tacgataaat atgcacgcct tgctggcgag cgactgcaat ttagtgacaa cgggttaaca
     3061 ccgtttggca cctgtattga tgaagtgtat tccgccaccg aaggccggat cggcaataaa
     3121 aaagtgattc tggccgggac caataattat ctcggtttaa cctttaatca tgatgccatc
     3181 agcgaaggtc aggcagcgtt agcggcccaa ggcaccggca ccaccggttc tcgtatggca
     3241 aacggcagtt atgcgccgca cctggcgtta gagaaagaga ttgccgagtt tttcaatcgc
     3301 cctaccgcta tcgttttttc aaccggttat accgctaatt taggcgtcat cagcgcactt
     3361 gccgaccata acgccgtggt gttactggat gccgacagcc acgccagtat ttatgacgcc
     3421 tgttctctcg gtggcgcgga aattattcgt ttccgccata acgatgctaa agatctggaa
     3481 cggcgcatgg ttcgcctcgg tgaacgggcg aaagaggtga ttatcatcgt tgagggcatc
     3541 tacagcatgc tcggtgacgt tgcgccgctg gcggaaattg ttgatatcaa acgtcgtctc
     3601 ggtggttatt tgattgtcga tgaagcgcat tcgtttggcg tgttaggcgc aacggggcgc
     3661 ggactggctg aagctgtcgg cgttgaagat gatgtggata ttatcgttgg tactttcagt
     3721 aaaagtctgg cctcgattgg cggttttgct gtcggttcgg aagcgatgga agtgctgcgc
     3781 tacggcagcc gcccgtatat ttttaccgcc tctccttctc cgtcctgcat tgcgaccgtg
     3841 cgctcgtcgt taagaactat tgccacacaa ccagagctgc ggcaaaagct gatggataac
     3901 gccaaccatc tgtatgacgg tctgcaaaaa ctgggttatg agttaagttc acatattagc
     3961 ccggtagtgc cagtaataat cggttcgaaa gaggaaggtt tacgcatctg gcgcgagctt
     4021 atctcgctgg gcgtgtatgt gaatcttatc ctgccgcctg ccgcgcctgc aggcatcacg
     4081 ctgctgcgct gtagcgtaaa tgctgcccac agtcgtgagc aaatcgatgc catcattcag
     4141 gcatttgcta ctctgaagca gtaatgtgag cccgccgtgg tgtttgcgcc acggcgggtt
     4201 taatgttgtc tgtcatacca atacaccagg ctcaacgcca ccaacacaaa cgctaccggt
     4261 aaaccgagcg cggtaacagg tgcgcttaac gcaaatagca atcccagatt aaccatgatt
     4321 tgatagctga tccaggtgag taagcccacg atgacgccta cagcgagacg gctacccatc
     4381 ccaggcgaac gcggggcagt aaaggtaaac ggcacagcca gcaaaatcat cgccagggtc
     4441 aggatgggtt gccccagttt ttcccacagt gcaaggcggt attcgctgct gggttgcccg
     4501 gtttcctgca aataatggat gtaatgatta agctgacgga cagaaaaagt attccccggc
     4561 attgataact cttcaagatt catactggtg aaggccgatt gccaggcaag attatctaat
     4621 gtttccagcg tttctttgcc attcagccat tttttatgat tcacaccatg caggacccac
     4681 gttttgtcgt ctttaatggt ggcactgcgt gcataaatgt aggattcaag cgagagatcg
     4741 tcgcgataat gaaatatctc cacgcccacc ggctggccct gctcgttcag cgatttcacc
     4801 gtcacaaatt cattaccacg cctggcccac agcatattgc cggtaatgtc atcgtcctcc
     4861 cccaacgcgg tggcggtcga tttgatttgc aacgcctgtt gctgcaatgg cgacgccacc
     4921 cactcatcta tcgcgcctaa cgaaacagtc cacaatatcc cggcaaccag cgccaccagt
     4981 gcaatacgga agatagaaaa ccccgtgctg cgaatggcgg taagctcact gtttttcgat
     5041 aactgcccca gtccaacaat accacccaat aacgcgataa acggcgaaag ttcgaccagt
     5101 gtgcgcggta aggtcattag caccaccagc accgcctgag tccagcgata accgcccggg
     5161 ctgacatcat ccagttcgtt aatcaggttg aaggtggtaa aaagcggcag caatagccct
     5221 gcggcggcgg caaaaccgag aaagagatga cggattaaat agcgactgaa aacattcatc
     5281 gggaaaattt ctgcagcaaa gagaagtcgc gtgcgaccag catcagcagg cccattagca
     5341 tgagtccggg tactaaccac aaaccaggaa tgaggggaat cgcgccgtta gccaccagcg
     5401 tacggcagac gtcgccgcca taaaaaatgg caataaacag caacgtcagt ggcaataatg
     5461 ttgaaaatcg cccttgccgc ggttttaccc gacttaatga aatggccagc agcgccatca
     5521 acaatgctgt caggccacgg ctttgtcgcc attgcagttc ggcatgatcg gcaggaaagg
     5581 cggagcgcgc cagctccgtg accgattttg ctttgcgttt gacgttaggg ctttgatcca
     5641 gcggattcag atgtaattgc agattacggt agatctgttc gttgtcgtca cgcccctgat
     5701 gatcgagaag ataggcagtg ccgttgtgca acataacggt cggtttctcg ggtgatgggt
     5761 caaccacatc aaccgaacgg gcgcggaaaa tgcgggttcg attggcagta gaagtataaa
     5821 tcagcgcgtc agtcaggcga ttattatcct gatcaaccgt ctgcgaaagg atcattcgtc
     5881 cgttatcgtt agtgttaaat ttctttgccc gcaactggcg aacatccagc tccgactgcg
     5941 actgctgctc cagttgataa atctgcgtat aagcccacgg acgaccatac atcgacagaa
     6001 gggtgacaaa aatacttagc ggtatcgcca gataaagaac ggctttgtac aagcgtcccg
     6061 gactgccccc cgccgcagag atagcggtaa tttccgagtc ggtatacatc tgccctagcg
     6121 tcacgccaac tgacacatac agaccaacag gtaacaacat ctccagtgca atcagcactt
     6181 tgtagaaaac gatatccagc acaacatcaa gagctaatgt cccatttgcc gcttcggtca
     6241 gataacgctg tgcggagtag ctggcgaaaa taaagatcag aaaaccgacg ataatcagca
     6301 ccagacggcg cgtaccacgc atgatgtagt gttcaaccag cttcatctca ctaacgccct
     6361 caatcctttg aattttttat tcattgatca gtttctgcgc cagcgcaatg acatgatcta
     6421 cctcttccgg cgtcaggctg ccgtcctttg cccactgaat ttgcccggct ttattcagca
     6481 ccagaatggt tgaactctgc tccggcaagc gccaggcaac gcgtccctgt ccattgccgt
     6541 caataatgaa ttgcgcccac ggatagcggc gtttattttt ttcgattttg ccaacaacaa
     6601 aatagccagt accaaagatg gcatcatcgg tgttcacaat ggtggtgggc tgaaaacgat
     6661 cctgcggaaa gtttgccgct tcaaccgctt taattaacag tgagttcttt ttcttcgccg
     6721 acttacgccc ggcgatatac tgaataatgc ggacttttcc tgctaattgt gtgctgttcc
     6781 acttttggta gtgaatttcg tcgtcgctat tcagaagcag ctcgccgcct tcctggatat
     6841 aaacaggggt aaccgttttg ccagtgacga aattgtgtgc ctgcacaccc ggggctaaga
     6901 gcatgataat caacgcaata atcaaacgag atgacatcat tgctccggct atagcggaaa
     6961 acgcgaaatc agcagctcta agcgctgacg aatatcgcta tccagcgcca acggtgcatc
     7021 atacgactgg cggttttcca tatcatccgc caccatgtcg tcgtgtttat ctcccatcag
     7081 gccattaatg gcggcgagga aacgggaaat ctgtaacgcc tctttatttt caggatcaag
     7141 cgatcttaag gtgtcgtaat aagaaacctg ttggctgatg atttttcgca catcatgaaa
     7201 ttccctgccg gttagtcgcg gtttggcaat aatctctttc agggtatgca tttgcttttg
     7261 ccgataggca ataaaactgc ttacgctttc taactggcta tggcgtaatg aatagttcac
     7321 caacgaccag accggagagc ttaaataaat ccgtaacagg ttgccgtaat acgacacaat
     7381 atttttctta ccgttacgaa agccgtcctg aaaacgcccc agaaaaacgg tcgttttgcc
     7441 gaataaaaat ccggcctgat gcttctttaa atacagccgt tgcgcaaaac gcaggtgctt
     7501 gtagcgcgct cgcatgtatt taaattgttt gcgctcatca gcagtcagct catcgccttt
     7561 ggcttgtttg cgaactaaac gcagtaattc ctgacgatca acgccattga gccagaattg
     7621 caggcataac gcgtaattat catgaatgat ttcggtggta caacttagtg caatagtcgg
     7681 cggcaaacta acctgtgcat caacgacatc atcttcttca atcgcaacaa atagacgctc
     7741 gctcgcctca agcgggaaat attttctcat catcaggatt tctctccggc tgcgttataa
     7801 agcaaggaat ggcgctgaaa aaatgccttt aaggttggaa ttaacgagag cgccacgtcc
     7861 agacattgcc caccgaagta agcaaacgct gctcccagca caccaaacag atgacagaac
     7921 atgaacagca atacgatgta gccgattgag gcgatggttt gcgccacgag aaaagcgcgc
     7981 tgttttcccg ccatcaatag caatgactcc tgagggaagc ccagcatcga aatcacaatt
     8041 gcgcccagca tcacctgaat caggtcatac gcttcgagat acttaacgcc aaacaccagc
     8101 gaaatgagcg gcttgccgac gataaacacc gcgagcgcca ccagaatacc gattccgccc
     8161 gccagtaaac cggacttcac acctaacaac cacggtctgg tggtgcgcgg atctaaacgc
     8221 atcacctctg ggtaaaaact tttacccagc aaacccgctg gcgttccggc ggcgtcgaag
     8281 aatgtcatgg cgattttaaa taacccggcg gcagcgggtc ctaacacgat ccccaccagc
     8341 actgtgctgc acgagttacg cgccgaccag atggagtggg caatgttggt tgaccagaca
     8401 aaactccacg cgcctttgat gtgtcgggca gactcaaaca gattcaattt gaaggcatta
     8461 tggatatttc tgcggcgtaa ttcgcgcgct gcaaaccacc agtacatggt gccgccaacc
     8521 agattcgaca cgtaccaggc aataacaaaa cccgcaaaac caaagtcaaa ataccaggct
     8581 acgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgcct gctgtacagc aattaaatcg
     8641 aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg aagccattga aggaatgagc
     8701 gtgcaataca gcgccgccag ccaaaagctt tggtcatcca gacctaatga atgggaaagg
     8761 aatggcagta aggcaatgcc gccgacaatc gccaccgcgc cgctgacgat atccagcgag
     8821 aaggaaaatg agacgacatt gcgaaattgc tgcggattat tgttggtcaa ggctggcgtt
     8881 ccgtactgaa ccaccagttg ccatgtctga aacttaataa aatcgctgat cgacttggcg
     8941 tacgattgca caatcaccag tacgccaaac atggcgggcg tcatcccttt accggcacac
     9001 gagagcgcca acagacccag caaggcgctc acgacattac tggagcctaa ccaggcgcta
     9061 ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat gtttgatgtt aaaacccgcc
     9121 aattcagcct gaccttatcg ttgatagtaa aaagtatccc gccagcctta agttaaactt
     9181 cggcggtcag aaacgatggc aaccagagaa accgccttct gtgcctgttc cagcacttcg
     9241 ctgtagggcg ctctggaatc aatctcaaga atttttgtgc cgttatagcc aatcgtcgac
     9301 atgacgccga ttttgtcctg cagctcggca tagtcatggt caggcttgcg ggagatggca
     9361 gtctcaatat caatgcccag gcgaataatt aattccgggc gatattgcgc catttgttgg
     9421 tataaacgcc gttcgcgctg cgccagaaac atgctgattt tcccggtcgc acgttcgacg
     9481 ccaatccccg gtccatcata ataaaagccc gaaatttcag cctgcgggaa gcgatcgctg
     9541 accaccagaa cgccactttg cgccagtcgc tgaaccttgc gtagattcgc cattcttcgc
     9601 agcgagaagc agtacataat caccgccgcc catagcgccg gagatttggt tttcatgctt
     9661 tgggttttcg aggatttggc cgccagtcgc cgttccagcc agacgccaac caacggcaat
     9721 cgtttgattt tgtcgccgtc ttcgccggag agcagcccca gatagcgccg ctcggtttgc
     9781 cagtgttgtt gcagcgattt caccaggtcg gtggtcagtg tggatttacc ggtgccatca
     9841 cacccgacca ccgcaatcag ccccggaatg tagttgggct gcggcgcggt tgtactatta
     9901 acagtttgag tttgtagtgc atccattcgt taaatcatcc cctatccatt ttggtgatca
     9961 ggaaagcgcg gcgtgaattg cgcgtagtga cgcttgcaga atttcatcgg cggggtgtcg
    10021 cccatccagt tcaaggatct ttgcgccatt gaatgtcagt tgcggcgtaa cggcgatttt
    10081 ttcctgtaac gctgccagtt ggtggtcagg tttacgcgca aacgcggttt gttcatcaat
    10141 gccaagacga atcaacaata cgggcaaata agatgccatc cattggtaca gcttcagctc
    10201 gcgctgcctt aacattttta tccaaccgtt accgcccgtg gtttttgcca attgcgggcc
    10261 atcaaagcga aaacccggca cttcaacctg cgggtagcgg tcggtgatga gcagaaagcc
    10321 ttgctggctt ttacacaaca ttttgcgaaa cttgtacgcc cgccagcagg aaagcagata
    10381 gataaccagc gcagtaatat tgccaggcgg tgttgagggc ttttcgtgca catgtgccgc
    10441 tttacttcgc agataacgcc caaaaggtgc gccaataaca gggagctgtg aaatccattc
    10501 gccaattcgc ccggacgatt gcccgagata aatgcgttct gttggcattc ttgctgccag
    10561 ttcatttacc aggcttgccg tgagggtcga tttacctgaa ccatcacatc ctacaatggc
    10621 aataacacgc actggcgtgg aattaattat tgacatatta cgttgattca cgaaaaaccc
    10681 ggcagtaaat aatgtattga atattacgtg gtccgtgctc agaatatccg ttcaacctta
    10741 ttgtgacaat ggttaattgt gacattgacc tgaattacgc tttcataaaa acatattaac
    10801 caaataaata ttttggatgg atatttaaat ttaaggatat attcatgcag tcaataacac
    10861 ctccattaat tgccgttatt ggtagcgatg gttcaggcaa gtcaacggtg tgtgaacatc
    10921 ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaagccg
    10981 gaaatgtcgg tcgtgcagtg acaaaattac cgttgatggg aaaatcctta cataaaacaa
    11041 ttgaacgaaa tcaggtgaaa acagcaaaaa aattgccagg accagttccg gcgctggtaa
    11101 ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc
    11161 gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg
    11221 gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg
    11281 aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg
    11341 ttgaccttga agttgcctgc gcgcgtaagc ctgaccacaa aagggagtcg ctggcaagaa
    11401 agatagccat aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca
    11461 atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattatggat tttatgaccg
    11521 cgcgtggtta tcactagcca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag
    11581 gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa aatcgccgcc
    11641 ggtaaagtaa atacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg
    11701 ccgtccacca ccatcgtccc tgcaactgca gacgagagga cgtgtgttgt ggagacgggc
    11761 atcccaatat aactggcaag accgatagac actgccgccg tcatttgtgc cgccatgcct
    11821 tgcgcatacg tcatgccgcg cttaccaatc ttctaccgat ggtcatcgct acacgacgcc
    11881 agccaatcat ggtgccaatg ccgagcgcca gtgctaccgc catgatgatc cacaccggag
    11941 cgtactcaat ggtgcttaac atatcgctgc gcagtttttt cagcaggttc tggtcttctt
    12001 tactgacgcc tggcagtttc gccagcttcg cggaggtatc tgagatgcac agcataatgc
    12061 ggcgcagctg gctgcgctga ctcacgctta acggctcgta actttccata ttgcctggca
    12121 gcatcgtttt aacgcgcgca atcgcatcaa aggtatttgc cggatgacag tgaaactctg
    12181 ttacttgcgc gccatcagtc gatgccgcag gcaatggagg ttccatcgca atcaacttct
    12241 gcggcagttc aggatgctgt tgcagatagt gttcgaagtt ggagacggca tcgcgagtac
    12301 gggtaatttc atagccggac gcgttcatat tgacgacgaa gccagcaggg gcaatcccca
    12361 ccagtaccag catcaccagg ccgatccctt tctgcccgtc gttcgcgccg tgcgaaaacg
    12421 ccacgcccgc agcggaaaca atcagcgcaa tacgcgtcca gaatggcggt ttacgtttgc
    12481 cttttttctt tttgcgatct tccggaatgc ggtgaatacg gtcacgcttt ttcgtcccgc
    12541 tccagaaacg gcgcagcagg aaaatcagac cacccgcaat gaccaggccg acgatagggg
    12601 aaacaatcag cgaggagaaa attttggtca cttcacgcag gtttaacgca tccatcaccg
    12661 atgagccggt taacagcgcg ttggttaaac cgatgccgat aatcgcgcca atcaaggtgt
    12721 gcgaactgga ggccggtaaa ccgaagaacc acgtccccag gttccatata atcgccgcca
    12781 gcagcatgga aaagaccatc gccagaccgt gggttgaccc catattcagc aacaaatcgg
    12841 ttggtaacat atggacaatg gcataggcaa cgctaagtcc gcccaataac acgccaaaaa
    12901 agttaaaaaa tgccgccatc accacagcaa gttgtggttg catggcacga gtataaataa
    12961 cggttgccac cgcattcgcc gtgtcatgaa aaccattgat tgcttcgtag aacaacacaa
    13021 atgccagagc aagcaataac aaaagccctg tgtatatatc aaggccaaca aataaattta
    13081 gcatatatta gattacgcca ttttgaattt acgaacggac gcattatcag tgactttaac
    13141 ggcatgggca aagtgaaata tcatttttta acgataaaaa gagtgttatt tcttgtgtat
    13201 cagacaataa gcattcattc aaaaccttta gaaataatca aaacacaggt attccactgg
    13261 ttaatatatt cacatatgaa atgaataaat attggaatat ataaatattg aatattttga
    13321 ttaatccccg tactaattat tcttcataat cagtacgggg ttgctacaac atgtattact
    13381 ttttcaccac aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac
    13441 cgcgcaaaca cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt
    13501 taccgtccac tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt
    13561 taccgctggt tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac
    13621 agcgaccggc aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga
    13681 aatcggtatc cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg
    13741 ctttgttgcc ggggatcacc gtccacagcg gctcaaagac tagcacttcc gggcgcagca
    13801 atacgtcgat aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa
    13861 actcacggtc gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc
    13921 acacgcagtt aaccagccgt ccttccccat caatcagttg cccggcagca tcccagccca
    13981 gttcatccag cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca
    14041 taaactgcgc gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac
    14101 gtgcacgact gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga
    14161 agccgttgcc tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat
    14221 gacatgaggc agagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga
    14281 aatccatacg accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg
    14341 gccagaggat tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt
    14401 cggttgcgtg aagatacatc aggtgcagct cgttggtggc tttaattagc tcctgctcgg
    14461 cactctcggt aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct
    14521 gcacataggc gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt
    14581 tgttttccag gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta
    14641 agctgtattc agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag
    14701 tgtctttcag ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt
    14761 gcccttgcgg caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat
    14821 gcaattgggt aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga
    14881 taagaagcgc acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca
    14941 ggatgttgtc attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac
    15001 ccacgtcagt aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc
    15061 attgccactt gtggcccata tattcatcgt cgatatagct acggaatacg gcgtcatctt
    15121 cgtattcctg cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg
    15181 cgtagcccaa taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt
    15241 tacctttaac aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca
    15301 gcgacattaa ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa
    15361 agggttaaaa aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc
    15421 gctcagaagg caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg
    15481 ggataaatcc gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca
    15541 tgaaaaaacg ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa
    15601 cggtcagaaa gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga
    15661 gtacgacgcc tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga
    15721 agtgaaccca aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg
    15781 cgtgtttgaa tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc
    15841 gcaggatttg gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc
    15901 accgttcctc ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta
    15961 cgccatcaac cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact
    16021 ggcgcagcat aagtttattg cgggcgatga gtacaccatt gcggatatgg cgatttggcc
    16081 gtggtttggc aatgtggtgt taggcggcgt gtatgatgcc gcagagtttc ttgatgcggg
    16141 cagttataag catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg
    16201 tgggcgtatt gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga
    16261 cgccagtgat ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt
    16321 cgcagcaagc catccaagcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat
    16381 gcctgatgcg acgctgccgc gtcttatcag tatctacgaa agctaatcat cacgcgctgg
    16441 taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc tttggcttca tcttcgtcga
    16501 tgatgctcat ggcaaatccg acgtgtacca gcacccactg gcccagtaga tcggcagggt
    16561 taccttcaca aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg
    16621 caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacatta
    16681 ttaactccgg ttattcaact tcaatacttt tgacgatcag cgaatcgccg gtatcgacac
    16741 gcaaccgctc gccgtgacag agcggacact gcgcatcgtg ctgatgaatc tccaccacct
    16801 ggctgcaatc ccagcaccaa gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt
    16861 gcgccaccgt tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc
    16921 aggagagcgc gccaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg
    16981 cctgccgttg gataatttca acggcgctct ggcaaagaga caactcatgc attttcgcca
    17041 ctcctgcgac caaacagcag ggcgcgacgc cctgcgtgtg gtacatccgg attcgtgact
    17101 ggcagcgaaa ggatcattcg tgcgcagtca tccgtcaggc gttggccctc ttcaatcgac
    17161 atgctgtgcg aaagcggcga catcagcgaa caggagagat attgcgaaac accgtccagt
    17221 tcgccaacgg taaaggtcat agtgccatac ggcagttgca gaccaatttt ttcactcact
    17281 ttgcgcagcg gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg
    17341 gtgatcacac accccgtcca ctgaccttcg aacagcgtaa aatcagaaac atacaccggc
    17401 attgaaggat gcagaaaaga gagatcgtgc atcgaacgcc gggcaatttc ttcaaacgct
    17461 gcctgtactt gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc
    17521 ctcccgtggg atggcttcca cgccagattc acgcagcgca gccagaacct gctcaagcgc
    17581 aggttcaatc attgcttcaa ccgtcggcgt taagccgatg tgtggctcca gcgattccgg
    17641 gatcacgccg accagggtca gctttttcgg aaactcaccg gtgaagcgca gggccgacaa
    17701 tacgtcggcc aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc
    17761 ttcatcccgc aggatcatca tcgttcccgg cgtgttcttt ttcgacacaa tggcatccgc
    17821 aataatcaga tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgtgccgcc
    17881 atcgaggatc tcaacataat ccggcagaat gtatcgttgc tctaacgctt cgacaatccg
    17941 cacaccgatg gcttcatcgg tcagcaaaat attgccgacc cctaagacta aaatacgcat
    18001 tacagaacct tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag
    18061 gccatgcacg ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatca
    18121 gcaaccggtg tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc
    18181 ggaccagagt tccaggttga tggaacaact gcctggtagt tgctgatgat accgtcttta
    18241 atcaccatcc agtgagagag cataccgcgc ggcgcttcga ggaagccaac acctttgaac
    18301 tcacccgttg ccggaatgtt cggtttcaca aaggtggtgt gatcgccttt gccgatattg
    18361 gtgatcagtg cactgtattg gttttgcagg atatcctgca attcacagca gtgaacggta
    18421 cgaccaataa tacggcccag cgtggagtgc agctgtgcca cttccagcgt gttgccagtc
    18481 agtttctgat aaatcgcaac gatttcattc agtttgttct gggtagattc tcgacctgcc
    18541 gccagtttca ccagcatatt agccagcgga cccacttcta ccgttttgcc gtagaaagtc
    18601 ggtgatttca cccaggaata tttcccgtcg tcagaccaac catcataagc cggaatggtg
    18661 gtaccttccc acggcgcctg tggcgcttcg tctttatacc aggagtgctt cgcgctttcc
    18721 tgaatgcctt tgatcaggta ttcatcggaa tgagaagtga tcggacgata cgaggacaga
    18781 tccgcattct caatgtagcc gcccgggaac aggaagctgc cgtttttgct gtcggtcggg
    18841 aattccggca cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag
    18901 aacgcggcga taaccgcggt atcaacctta taaacctgct caacaaagtc gctcagtttg
    18961 tcgatgaaag acttgatgta catcaggcgc tcaaggttca acacgcccaa cccgtcgagg
    19021 ttgattgggt tcgcgacacc acccactgcc aggttctgaa tgtgcggcgt tttaccgccc
    19081 agcagcgcca cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct
    19141 accgcaatca ggttcacttc cggcggcagt ttcatcgctg ggtgacccca gtagccatta
    19201 gcgaaaatac ccaactgacc gctggcaacc agatctttga tcttgttctg aactttagtg
    19261 aactcttccg gactgttcag atgccaggtc gaaacgcctt tcagcatttc ggaggctttg
    19321 gttgggtcag cttgcagtgc agaagtgatg tccacccagt ccagcgccga aagctgatag
    19381 aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc
    19441 gcgttaaccg gaacgtcgat attcagcgca ctttctgccg cacgaacgga agacagcgcg
    19501 tgagtggtag tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg
    19561 cggtttttca cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa
    19621 acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga
    19681 tcaatagtaa ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt
    19741 ttaaaggagg aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa atctcaatag
    19801 ccacaaaacc aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc
    19861 ccgggttgaa tgccaccagc gaataggtca gacgccaggt tgcacaacct aacagtgcgc
    19921 tcagtgctga caggaacagc atgcgggaat cattacgcag cttcgccaca cgcagaacca
    19981 ccagcgggaa gagcatcagc aggacttcaa tccagaacat cacggagtag aagtcaccgg
    20041 caaacgctaa cgacagcttg tcgcgataga taagctcgcc aaagcgcagc acgatgaaaa
    20101 tcgccagcaa cacactgatg gtgttggtca gcttgacgaa cagactcttt tcatccggac
    20161 cgttgccacg cagacccgcc tgcaccagcg aaccttcaaa gatgacaatc gagaagccca
    20221 tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc atagctctgc cacaacggat
    20281 gcaccttgta gcccgccgag atcatcagtg accccattga agactggtgc atggtcggca
    20341 acagcgcgcc aagtgcaatg atgaagaaca tcaccttgtt cagacgcttg agagaaacct
    20401 tccagcccag acgttcaaac agtgccggag caaactccag tgccatcacg ccgatataga
    20461 tggtcataca gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt
    20521 agaagtacgg caggttccag tagcgaccca cgtcgatagt gatcgacaag ccccccagtg
    20581 agtaaccaaa cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt
    20641 taaagacgta taccgcccac gccagcgccc agccgccaca ggcaaagccg gtaccaatca
    20701 gcaggtcaaa cgcgatccac acgccccacg ggaagccgcc gttcaggtca gagacagagc
    20761 ccagaccgaa caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa
    20821 aaatcatgac cggtttactg atgattttgc cgcccagcgg ttgtggatca tgactcatga
    20881 tcgtctcctc cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac ggttaagccc
    20941 gccagcacag ccagtggtag catcatgcct ttatacaggg tgtgttgaat attttcggaa
    21001 cgcgcaccgg tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc
    21061 gtcagtacca gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat
    21121 ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat
    21181 tcgctgccag gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta
    21241 ccaaaaatta ccgcgcccgc cgggcacact tctacgcagc caggcaaacc gcctttatcg
    21301 agacgttcca cacctttctg gttgcacaac tcgcacttat gcagcgcacc aaacgggttg
    21361 ttgtagtcgt acttcggcac gttgtatggg caggcgacca tgcagtaacg gcaaccggtg
    21421 cacacatctt tgtcgtaatg gacaatgccg gttttcggat cttttttcag cgcagagacc
    21481 gggcacacag agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg
    21541 tagccgttct cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg
    21601 ttattggtat acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta
    21661 cgttcaggga agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag
    21721 gtcgagtcgt acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca
    21781 tgactgacag acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa
    21841 ttacgtctgt tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac
    21901 ccagttcacg caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca
    21961 aaccaatagc gcctgcagag acgttgccgc cctctttagc gttaacatcc ggtttctgtg
    22021 aacgcggagt ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac
    22081 cttcttcgtt acagccatag catgggtgac caatcgctac cggccacacg ccgccaacat
    22141 cgcagaattg cagcgttgag cagttgccgt aagtttctgg ccctttacag ccgaggtggt
    22201 acaggcacca gccttcacgg tggccttcat caccgaactc tttggcaaaa cgaccagcat
    22261 cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac
    22321 gatttttgtc atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca acggtcgcga
    22381 ggaagttgtg cgggttcggc gggcagcccg gaatattgat aacggttttg cctggcagaa
    22441 cttcttgcag gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg
    22501 cagagcagga accgatagcg ataatcgctg ctgcgccttc tgccgctttg cggatgtgat
    22561 ccacaatcgg ctcaccagca accatgcaat aaataccgtt atcttttaat gggatggaac
    22621 catccacaac caacacatac tgccctttgt acttctcaag tgcgttatgt ttgttctctt
    22681 cgacctgatg accgaaggcg gcggaaagca cttcgtgata ctccagagag atagtctcca
    22741 gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc
    22801 actcctgcgc gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca
    22861 tctctgcagc ggctttgcca cttaacccca tggtggcggc taatgctgca caaagcttca
    22921 tgaaatcacg acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata
    22981 gttattccgt tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga
    23041 tcgatgcgcc atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg
    23101 cgctaaaacg aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt
    23161 tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt
    23221 ctcacgaaag aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc
    23281 cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc
    23341 taatacaaaa ccgcgtttat aatcggcaca gaattttgtc acattaaccg cactgtcggc
    23401 atgcgttgct tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct
    23461 cattcgctat atatcgccat atatagcgat attttaggca aagaacgact tttgcgcacg
    23521 ctattttttc atgcttgttt aagacgtaat ccagtaagag ataaacctat caggagagat
    23581 atttatcgaa ccatttcagt gtcctttgcc aggcaagatc ggcggcagat ttgtcataac
    23641 ggggcgtgga atcattatgg aatccgtgat taacccccgg atagatatac gcctcataaa
    23701 ccttattatt ggctttcaac gccgcctcgt aagcaggcca gccctcgttg attcgggtgt
    23761 ccagttccgc gaagtggagt agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg
    23821 cctgacgacc ataaaacggc accgcgcagg ccagttccgg atacgcgaca gccgccgcgt
    23881 tcgatacgcc accgccatag caaaatccgg taatacccac tttgcctgcc gcttgcggat
    23941 agcgttgcat aaactcaatt gcggcaaaga aatcattcat cagtttggtt ggatcaacct
    24001 gctgttgcag ctcacgacct ttatcatcat ttcccggata acctccaacg gaacttaagc
    24061 cgtcaggtgc cagggcgata taccccgcct tcgccactcg ccgtgccaca tcttcgatat
    24121 acggattcag tccacgattc tcatgcacca ctaccacggc tggcgttttg ccgctcattt
    24181 ttgcgggttt caccagataa ccccgtacct cgccgtgacc atttggcgaa ggatacgtga
    24241 tgtactcagc aacaatctcc gggtcggtaa actctacctg agtcgccagc gcataatttg
    24301 gcttgagcaa atcaaacaac gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa
    24361 ggttgaggaa ctcacgtttc gagattttcc cgtgagcgta atagtcgtag taatccaaca
    24421 actcttgtgg gaaatctttg gcggttaaac gcggcatcgt tgcactcctc aactggtgtt
    24481 tttttaagca aagcataagc acgtgttttt gcccagtttt tcgtcactct gtgagccaga
    24541 ctacgggata cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacggtg
    24601 gaaaggagag gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcaataccg
    24661 ctattgcgga aaaagcggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt
    24721 cggtcacgtt aacgcgctgg aatcacagcg tgcgatcctg cgtaaagcgt ttgatttggg
    24781 cattacgcac tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa
    24841 ctttggtcgc ctgctgcggg aggattttgc cgcttatcgc gatgaactga ttatctctac
    24901 caaggctggc tacgatatgt ggcccggccc ttacggctct ggcggttcac gtaaatacct
    24961 gctcgccagc ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta
    25021 ctctcatcgc gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc
    25081 ggtacaaagc ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca
    25141 aaaaatggtc gagttgctgc acgagtggaa aattccgctt ttaattcatc aaccttcgta
    25201 caatttactg aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg
    25261 cgtgggctgt attgccttta ctcctctggc tcagggattg ctgaccggaa aatatctcaa
    25321 cggcattccg caagattcac ggatgtatcg tgaagggaat aaagttcgtg gtctgacacc
    25381 gaaaatgctt accgaagcca acctcaacag cctgcgctta ttgaatgaaa tggcacagca
    25441 gcgtggacaa tcaatggcgc aaatggcgtt aagctggttg ttgaaagatg atcgcgtgac
    25501 gtcggtattg attggtgcca gccgcgcgga gcaactagag gagaacgtgc aggcgctgaa
    25561 taatctgaca tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggcga
    25621 gctgaatctg tggcaggcgt cgtccgataa atgacctgtt aataacgggc gcgcgaaccg
    25681 cgcccgaatc agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg
    25741 cagaaagcac aaacgtcaga tggataatga cgtaccacat cagtttgtta tccgggacat
    25801 ttttcgcatc cataaagacg cgcagtaggt gaatggaaga aattgccaca atcgacgctg
    25861 ctactttgtt tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt
    25921 tctcggagat atccagctgt gagacgaaat tctcataacc ggaaaacatc accatcacca
    25981 gcaaaccgcc aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt
    26041 ccgccatcga gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca
    26101 gggcaactaa cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat
    26161 acattgcatt ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca
    26221 gtatatcgca attgagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg
    26281 tcgcataatt ctgaggttta gggatcttcc tggttccctt atcaacgcat cgagagcgtc
    26341 tacccttaaa ggacgttgaa cctgaaggga gaaaaacgat gtctcattta aaagacccga
    26401 ccacgcagta ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg
    26461 cgaagatgac accggtaccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga
    26521 aagatcgtaa agcactggtg acagggggcg attccggaat aggtcgcgct gccgccatcg
    26581 cttacgcgcg tgaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg
    26641 ctcaggatgt gaaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg
    26701 atttaagcga tgagaaattc gcccgttcgc tggttcacga agcgcacaag gcgttaggcg
    26761 ggctggatat tatggcgctg gtcgccggga aacaggttgc cattccggat attgcagacc
    26821 tcaccagcga acagtttcaa aagacctttg ccattaacgt tttcgcgctg ttctggctaa
    26881 cccaggaagc gatccccctg ctaccgaaag gtgcaagtat catcaccact tcgtcaatcc
    26941 aggcatacca gccaagtccg catttactgg actatgcggc tacgaaggcg gcgattctga
    27001 actacagccg tggcctggca aaacaggtcg cggagaaagg tattcgggtg aatattgtcg
    27061 cgccaggccc tatctggacg gcactgcaaa tttccggcgg acaaacgcag gataagatcc
    27121 cgcagtttgg tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg
    27181 tatatgttta tctggcaagt caggagtcga gctacgtcac cgcagaagtg cacggtgtgt
    27241 gcggcggcga gcatttaggt taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt
    27301 gtgaattcac tcttacctga tgtggcgaaa acgccttatc cgcctacaaa cgcatgaaga
    27361 ttcaacatgt tgcaatagat tttgtaggcc tgataagcgt agcgcatcag gcattgagca
    27421 cctaatgccg gatgcggcgt gaacgcctta tccggcctac aaacgcacga agattcaaca
    27481 tgttgcaata gatattgtag gcctgatgag cgtagcgcat caggcaattc tactttactt
    27541 cgctttggcg gtttcttcgc cgaccagacc tatcttcagg taacccgcct gatgcagcgt
    27601 atccattacc ttcatcaacg tctcgtaatc gacggtttta tccgctcgga agaagatggt
    27661 ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac
    27721 cggatcgtta ccgataaaca tcgagttgtc tgccttcacc gacagataaa ccggtttttc
    27781 cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc accttcacat ctaccgtcgc
    27841 taacggtgcc gccaccataa agataatcag cagaaccaac atcacgtcga taaacggcgt
    27901 cacgttgata tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcattgccat
    27961 cggatattat cctgcgcgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc
    28021 ttccagatct aggtcacggc tttgcagcaa caatacctgc gctgcaacat cacccagcat
    28081 cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag ataacgaccg caggaatcgc
    28141 tgccacgagg ccgattgccg ttgctaacag agcttctgcg atacccggcg caacgactgc
    28201 caggttagtg gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt
    28261 accaaacaga ccaacgaacg gagaaatcgc gccgatggtt gccagatagc cgttaccgcg
    28321 gcccatttga cgacccactg cggcgacccg acgctccaga cggaagctgg tacgttcttt
    28381 aataccttcg ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag
    28441 caaatgcagg cttaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa
    28501 ggaacgcgct tcagccagca gttgctgctc gcgcttaagg cgacgcttct gattgaagaa
    28561 ctctacgctc ttactgaaga agattgccca agtgactacg gaggccaaaa taagcccaat
    28621 catcacgcac ttaacgacaa tatcggcgtg ctgatacata ccccagacgg aaaggtccgt
    28681 ctgcattaaa ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgcgcata
    28741 ataatatcaa aatgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta
    28801 cctttgcttt cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga
    28861 aaattttctg ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag
    28921 acatccagac gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac
    28981 tggtgaatgc aggacgcagc aaaaaataca ctctcggcgc ggtaaatagc gtgattcagc
    29041 gcgcttcttc gctggtcttt gacagtgtgg aagccaaaaa gcacgcgacg cgcaatcgcg
    29101 ccaatggcga gttgttctat ggacggcgcg gaacgttaac ccatttctcc ttacaacaag
    29161 cgatgtgtga actggaaggt ggcgcaggct gcgcgctatt tccctgcggg gcggcagcgg
    29221 ttgctaattc cattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg
    29281 cctatgaacc gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat
    29341 cgtggtttga tccgctgatt ggtgccgata tcgttaagca tctgcagcca aacactaaaa
    29401 tcgtgtttct ggaatcgcca ggctccatca ccatggaagt ccacgacgtt ccggcgattg
    29461 ttgccgccgt acgcagtgtg gtgccggatg ccatcattat gatcgacaac acctgggcag
    29521 ccggtgtgct gtttaaggcg ctggattttg gcatcgatgt ttctattcaa gccgccacca
    29581 aatatctggt tgggcattca gatgcgatga ttggcactgc cgtgtgcaat gcccgttgct
    29641 gggagcagct acgggaaaac gcctatctga tgggccagat ggtcgatgcc gataccgcct
    29701 atataaccag ccgtggcctg cgcacattag gtgtgcgttt gcgtcaacat catgaaagca
    29761 gtctgaaagt ggctgaatgg ctggcagaac atccgcaagt tgcgcgagtt aaccaccctg
    29821 ctctgcctgg cagtaaaggc cacgaattct ggaaacgaga ctttacaggc agcagcgggc
    29881 tattttcctt tgtgcttaag aaaaaactca ataatgaaga gctggcgaac tatctggata
    29941 acttcagttt attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcga
    30001 atcaaccaga acatatcgcc gccattcgcc cccaaggcga gatcgatttt agcgggacct
    30061 tgattcgcct gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg
    30121 gttttgcgcg aattgtataa cattgccact tttggacaat tttgcagaca tttaattgta
    30181 aaaagtctta aattgttgcg tccgggatca aggcgtcccg gacgaatcag gagtacaata
    30241 ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc
    30301 atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg atcctcatat tgttagcgtt
    30361 gtttactttg tcatgtttgc cacgctgttt ttagaaaacg gcctgctgcc cgcctcattt
    30421 ttgccaggcg acagcttgtt gatactggca ggcgcattga ttgcccaagg ggttatggat
    30481 tttctgccta cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat
    30541 attcaggggc gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct
    30601 gctaaatatc accagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg
    30661 gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt
    30721 ctgccaaacc gccgcttcca gtttttcaac tggttaagtg gattgctgtg ggtcagcgtg
    30781 gtaaccagtt ttggctatgc cttaagtatg attccgttcg ttaaacgcca tgaagatcag
    30841 gtaatgacgt tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttaggcacg
    30901 ctgtttgtgg tgattaaaaa aaaatactgt aacgcctgac gattttcccc gttcccggct
    30961 gctgtaccgg gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc
    31021 cggcgtcaca ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc
    31081 gacgcgcatc gctgctgcgc tggccttcat gccgtcatgg atgatcatca tccgcgcctt
    31141 atgcagacgg taattcttca aatactgcaa cggcgaggtg ctggtgacag acttaaaatt
    31201 atggtggaac gccgatacgc tcatgttggc ttctgccgcc agttgctcga cgctcaggtt
    31261 ttcggtgtat ttattctcaa tccgtttcag cacgcggcta atcagactga agtgagtctg
    31321 gcgactgacc agcgccagta acgcgccgcc gcaaggtccg gtcagcacgt agtacagaat
    31381 ttcgcggatg atctgtttgc cgagaatacg cgcatccagt ggtcgctcca tcacgtcgag
    31441 taaccgctcc gccgcgcata aaatctcttc tgataacgtg gcggagttaa tcccgctggc
    31501 tgccatcgac ggctggaaat gctcatcttc gccaatgtcc atcaacagtt cctgtaactg
    31561 caaaatatcg acattgagac gcaaccctgc cagcggcacc tctgacgtgg cataggtttc
    31621 gcactcaaac ggcaacggca ccgtcagcag caggtattca ttggcatcat aacgaaacac
    31681 gcgttcattg atataaccga ttttatgccc ggaaaagaga attatgatgc caggctcgta
    31741 catcaccggt gtacgtgcga aaggcgtctc gccatacaac aaacgtacat cgggcaacag
    31801 ttctgacaaa ctattttctt taattttcag tttattaact ttatccgcca gcaagcggca
    31861 aatctcttca cgtttcatat cgcgtaattt cttaggaata atgcggcaat ttgattgtgc
    31921 acaattttgt agcatttctc cagcactctg gaggaatagg caagacattg gcagaaatga
    31981 gcattgagag ccagggcgct ggcgatcaca atgaaaaaca tcaggcagat cgttctctgc
    32041 cctcatattg gcccagcaaa gggagcaagt aatgaacaac tttaatctgc acaccccaac
    32101 ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc gaacaaattc ctcacgatgc
    32161 tcgcgtattg attacctacg gtggcggcag cgtgaaaaaa accggcgttc tcgatcaagt
    32221 tctggatgcc ctgaaaggca tggacgtgct ggaatttggc ggtattgagc caaacccggc
    32281 ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa cagaaagtga ctttcctgct
    32341 ggcggttggc ggcggttctg tactggacgg caccaaattt atcgccgcag cggctaacta
    32401 tccggaaaat atcgatccgt ggcacattct gcaaacgggc ggtaaagaga ttaaaagcgc
    32461 catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt tcagaatcca acgcaggcgc
    32521 ggtgatctcc cgtaaaacca caggcgacaa gcaggcgttc cattctgccc atgttcagcc
    32581 ggtatttgcc gtgctcgatc cggtttatac ctacaccctg ccgccgcgtc aggtggctaa
    32641 cggcgtagtg gacgcctttg tacacaccgt ggaacagtat gttaccaaac cggttgatgc
    32701 caaaattcag gaccgtttcg cagaaggcat tttgctgacg ctgatcgaag atggtccgaa
    32761 agccctgaaa gagccagaaa actacgatgt gcgcgccaac gtcatgtggg cggcgacgca
    32821 ggcgctgaac ggtttgattg gcgctggcgt accgcaggac tgggcaacgc atatgctggg
    32881 ccacgaactg actgcgatgc acggtctgga tcacgcgcaa acactggcta tcgtcctgcc
    32941 tgcactgtgg aatgaaaaac gcgagaccaa gcgcgctaag ctgctgcaat atgctgaacg
    33001 cgtctggaac atcactgaag gttccgatga tgagcgtatt gacgccgcga ttgccgcaac
    33061 ccgcaatttc tttgagcaat taggcgtgcc gacccacctc tccgactacg gtctggacgg
    33121 cagctccatc ccggctttgc tgaaaaaact ggaagagcac ggcatgaccc aactgggcga
    33181 aaatcatgac attacgttgg atgtcagccg ccgtatatac gaagccgccc gctaagcttt
    33241 ttacgcctca aactttcgtt ttcgggcatt tcgtccagac ttaagttcac aacacctcac
    33301 cggagcctgc tccggtgagt tcatataaag gaggaacgta tggctaatcc aaccgttatt
    33361 aagctacagg atggcaatgt catgccccag ctgggactgg gcgtctggca agcaagtaat
    33421 gaggaagtaa tcaccgccat tcaaaaagcg ttagaagtgg gttatcgctc gattgatacc
    33481 gccgcggcct acaagaacga agaaggtgtc ggcaaagccc tgaaaaatgc ctcagtcaac
    33541 agagaagaac tgttcatcac cactaagctg tggaacgacg accacaagcg cccccgcgaa
    33601 gccctgctcg acagcctgaa aaaactccag cttgattata tcgacctcta cttaatgcac
    33661 tggcccgttc ccgctatcga ccattatgtc gaagcatgga aaggcatgat cgaattgcaa
    33721 aaagagggat taatcaaaag catcggcgtg tgcaacttcc agatccatca cctgcaacgc
    33781 ctgattgatg aaactggcgt gacgcctgtg ataaaccaga tcgaacttca tccgctgatg
    33841 caacaacgcc agctacacgc ctggaacgcg acacacaaaa tccagaccga atcctggagc
    33901 ccattagcgc aaggagggaa aggcgttttc gatcagaaag tcattcgcga tctggcagat
    33961 aaatacggca aaaccccggc gcagattgtt atccgctggc atctggatag cggcctggtg
    34021 gtgatcccga aatcggtcac accttcacgt attgccgaaa actttgatgt ctgggatttc
    34081 cgtctcgaca aagacgaact cggcgaaatt gcaaaactcg atcagggcaa gcgtctcggt
    34141 cccgatcctg accagttcgg cggctaacat gcaaattctc ccggtggcgg taatgttccg
    34201 ctaccggact tttcagaaat catttattcc cctcgcgtcc cgcccgttgt tactcttcct
    34261 tgttcaggaa tgccaaatat aaggacatca tcatgcagag ccggaagctc ttaaaagaac
    34321 aactcatcta tatccgggat aaacgcaacg gagaggtgaa aaacagatga aaataatact
    34381 tctgttttta gcagccctgg caagttttac cgtacacgca cagcccccct cactgaccgt
    34441 agaacaaaca gtccggcata tttatcagaa ctataaatca gatgccactg ccccttattt
    34501 tggtgaaacc ggagagcggg cgataacttc tgcgcgtatt caacaggcgc ttaccctgaa
    34561 cgacaatctt acgctgccgg gcaatattgg ctggctggat tatgatccgg tttgtgattg
    34621 tcaggatttt ggcgatctgg tgctagaaag cgttgcgata acccaaactg acgccgatca
    34681 tgccgatgcc gttgtgcgct ttcgtatctt taaagatgat aaagaaaaga ccacgcagac
    34741 actgaaaatg gtggcggaaa atggtcgttg ggtcattgac gatattgtca gcaatcatgg
    34801 cagcgtctta caagcagtta atagcgagaa tgaaaaaacg ctggccgctt tagcttcgtt
    34861 gcaaaaagaa cagccggaag cctttgttgc cgaactcttt gaacatattg ctgattatag
    34921 ctggccgtgg acgtgggtgg tttccgactc ttaccgccag gcggttaatg ccttctataa
    34981 aaccaccttc aagacggcca ataatcccga tgaagatatg caaatagaac ggcaatttat
    35041 ttacgacaat ccgatctgtt ttggcgaaga gtcgctattt tcacgcgttg atgaaattcg
    35101 agtcctggag aaaaccgccg attccgcccg tattcatgtt cgttttacgc tgaccaatgg
    35161 caacaacgaa gagcaagaac tggttttaca gcggcgcgaa ggcaagtggg aaatcgctga
    35221 ttttatccgc ccgaacagcg gcagcctact taagcagatt gaggcaaaaa ccgccgccag
    35281 attaaagcaa tgagctgaat taaataacaa ttagccggaa caataaataa aagggaacac
    35341 tatatgaaaa cgattttcac cgtgggagct gttgttctgg caacctgctt gctcagtggc
    35401 tgcgtcaatg agcaaaaggt caatcagctg gcgagcaatg tgcaaacatt aaatgccaaa
    35461 atcgcccggc ttgagcagga tatgaaagca ctacgcccac aaatctatgc tgccaaatcc
    35521 gaagctaaca gagccaatac gcgtcttgat gctcaggact attttgattg cctgcgctgc
    35581 ttgcgtatgt acgcagaatg ataaaaaaat ccccggcagc atgtcagttg ccggggattt
    35641 tttttaacgt ccaaccgccg ctttagggcg tttcttcgca ccagcattca ccggacgaga
    35701 ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga cgctgggtcg ccatcggcgt
    35761 atgtttcgtc aacgccggac gggtattgcg gttctggcga cgagcttcac gcatctcttc
    35821 aatggttggc gcaggcacta agcaatcgcg acggctgcca atcagatgct ttttgcccat
    35881 cgcttccagc gcctggcgga ttaacggcca gtttgccgga tcgtggtaac gcaacaacgc
    35941 tttatgcaaa cgacgctgtt tgtcgccctt cggtacgaag acgtcttcgc tcttataacc
    36001 aatcttcgcc agcgggtttt tcccggtgta atacatggtg gttgagttcg ccagcggcga
    36061 tgggtagaag ttctgtacct ggtcgagacg gaaacgatgc tttttcagcc acagcgccag
    36121 attcaccata tcttcatcac gcgtaccggg gtgcgcggag atgaaatagg ggatcagata
    36181 ctgctcttta cctgcctgtt ttgagtaagt atcgaacagc tctttaaagc ggtcatatgt
    36241 aaccaggtga atcatttgat accaacgagg gttttagtga aaggaggtga aatcacctgt
    36301 aagatactga aataaaacag gtgatgtctg gatgagcgct taaagtctat ttgataccga
    36361 ttgtgcaata attaatcgtt tattctaaaa tcggcaaaac ttttgatact gcgatctcaa
    36421 ctttttaaag cgtttaaaac ttcattaaat ctatatggca actaacatac gggccagcct
    36481 cgccaaacta acccgcttta gattctaccg gatttgattg cctacttgtc tcggtttttt
    36541 ccaaaatctc tctgacgcaa tcgtctggca tatctacaat catcaacgca gccaggcgct
    36601 tcctgccttc tagttgtaat aaatcctggt tttctttatc cagcagtgtc agaaccgtct
    36661 ctacaccttt gcgagcaagg agattggtta actgttctcg ttcgtctttc gtaaggaatg
    36721 aaagcatctg tgtaagttga tctcccttat caccagaaca accaaaatta ccacaaatac
    36781 caacatcgtt tggtgattca cccttgccct ctttaagcca ctcaagacta actccttcac
    36841 tttcagaaat tcgtataagg atactttctc tgggggctgg ttgtgtttgt cctcccttga
    36901 aatagctgtt taatgtgttg atattgattc cccacgctct ggcagctgca cttcttgaag
    36961 ggtagcgctt tgtaagcatt ccaatacgtt caataacagg ttcttttccc tgcgacataa
    37021 agatacctct tggtgctttt ctaaggaaag gtttctttct atttatccaa ttgaaaaata
    37081 aggacaaaag caaaaattag ccaaaatacc aaaaatacct tgatttggta tttttagtga
    37141 tgcattattt acccgtgcag atatccgcac gaatatccgc gcgggtaact ttttagaata
    37201 gaggaagtat gagtagaaga gaagtttcag atcacgattg gccgcctgaa atcatcaagg
    37261 cgcgcttaca catggctggc ttatcactgc gctctttatc tttaaaggct ggttacagca
    37321 gggattcgct gaaaagcgta ttacgtactc cttgccgacc gtatcagcaa ataattgctg
    37381 atgctcttgg ggtatcgcct gaagaaatct ggcccagtcg ataccaggtt aagagctata
    37441 tgagaaaggc gtcatgatat gtatgtcatt gcaaaagaac tgattggcgc gcccggaatg
    37501 cctgctacaa caaaaggtat tcgccaggca ttacaacgtt acgtacaagg gaaaagctgt
    37561 tgttcccgtc gtcgctcagg ctctaaagca actgaataca gcatcgactg tttacctgaa
    37621 gtgacgcagc aggcattacg tgaacgctac gccctgcaac tgatgacgca aaaagtcgat
    37681 gaatcaccgg ctccggtggt gacaaaggcc agacgctc
//
LOCUS       DAZUFB010000039        36483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZUFB010000039 DAZUFB010000000
VERSION     DAZUFB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.039"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(383..673)
                     /gene="nadS"
                     /locus_tag="ACHY3M_002202"
     CDS             complement(383..673)
                     /gene="nadS"
                     /locus_tag="ACHY3M_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS4317115.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(674..985)
                     /locus_tag="ACHY3M_002203"
     CDS             complement(674..985)
                     /locus_tag="ACHY3M_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4317116.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1214..2122
                     /locus_tag="ACHY3M_002204"
     CDS             1214..2122
                     /locus_tag="ACHY3M_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4317117.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="ACHY3M_002205"
                     /gene_synonym="yiiD"
     CDS             complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="ACHY3M_002205"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFS4317118.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="ACHY3M_002206"
     CDS             complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="ACHY3M_002206"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4317119.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3606..4478)
                     /gene="yihY"
                     /locus_tag="ACHY3M_002207"
     CDS             complement(3606..4478)
                     /gene="yihY"
                     /locus_tag="ACHY3M_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFS4317120.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="ACHY3M_002208"
     CDS             complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="ACHY3M_002208"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFS4317121.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5170..5955)
                     /gene="csqR"
                     /locus_tag="ACHY3M_002209"
     CDS             complement(5170..5955)
                     /gene="csqR"
                     /locus_tag="ACHY3M_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4317122.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5989..6885)
                     /gene="yihV"
                     /locus_tag="ACHY3M_002210"
     CDS             complement(5989..6885)
                     /gene="yihV"
                     /locus_tag="ACHY3M_002210"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFS4317123.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELMALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7053..7949
                     /gene="yihU"
                     /locus_tag="ACHY3M_002211"
     CDS             7053..7949
                     /gene="yihU"
                     /locus_tag="ACHY3M_002211"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFS4317124.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7973..8851
                     /gene="yihT"
                     /locus_tag="ACHY3M_002212"
     CDS             7973..8851
                     /gene="yihT"
                     /locus_tag="ACHY3M_002212"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFS4317125.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8868..10109
                     /gene="yihS"
                     /locus_tag="ACHY3M_002213"
     CDS             8868..10109
                     /gene="yihS"
                     /locus_tag="ACHY3M_002213"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFS4317126.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10247..11149
                     /gene="yihR"
                     /locus_tag="ACHY3M_002214"
     CDS             10247..11149
                     /gene="yihR"
                     /locus_tag="ACHY3M_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFS4317127.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11348..13384
                     /gene="yihQ"
                     /locus_tag="ACHY3M_002215"
     CDS             11348..13384
                     /gene="yihQ"
                     /locus_tag="ACHY3M_002215"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFS4317128.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENADGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            13430..14815
                     /gene="yihP"
                     /locus_tag="ACHY3M_002216"
     CDS             13430..14815
                     /gene="yihP"
                     /locus_tag="ACHY3M_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4317129.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14858..16261
                     /gene="yihO"
                     /locus_tag="ACHY3M_002217"
     CDS             14858..16261
                     /gene="yihO"
                     /locus_tag="ACHY3M_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4317130.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            16329..17021
                     /gene="ompL"
                     /locus_tag="ACHY3M_002218"
     CDS             16329..17021
                     /gene="ompL"
                     /locus_tag="ACHY3M_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFS4317131.1"
                     /translation="MKKINAIILLSSLTSASVFAGTYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17112..18377)
                     /gene="yihN"
                     /locus_tag="ACHY3M_002219"
     CDS             complement(17112..18377)
                     /gene="yihN"
                     /locus_tag="ACHY3M_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4317132.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(18479..19459)
                     /gene="yihM"
                     /locus_tag="ACHY3M_002220"
     CDS             complement(18479..19459)
                     /gene="yihM"
                     /locus_tag="ACHY3M_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS4317133.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDVRLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(19467..20177)
                     /gene="yihL"
                     /locus_tag="ACHY3M_002221"
     CDS             complement(19467..20177)
                     /gene="yihL"
                     /locus_tag="ACHY3M_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4317134.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(20393..22216)
                     /gene="typA"
                     /locus_tag="ACHY3M_002222"
     CDS             complement(20393..22216)
                     /gene="typA"
                     /locus_tag="ACHY3M_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFS4317135.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            22589..23998
                     /gene="glnA"
                     /locus_tag="ACHY3M_002223"
     CDS             22589..23998
                     /gene="glnA"
                     /locus_tag="ACHY3M_002223"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFS4317136.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            24317..25366
                     /gene="glnL"
                     /locus_tag="ACHY3M_002224"
     CDS             24317..25366
                     /gene="glnL"
                     /locus_tag="ACHY3M_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFS4317137.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            25378..26787
                     /gene="glnG"
                     /locus_tag="ACHY3M_002225"
     CDS             25378..26787
                     /gene="glnG"
                     /locus_tag="ACHY3M_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFS4317138.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDNWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            26899..27009
                     /gene="yshB"
                     /locus_tag="ACHY3M_002226"
     CDS             26899..27009
                     /gene="yshB"
                     /locus_tag="ACHY3M_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFS4317139.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27199..28572)
                     /gene="hemN"
                     /locus_tag="ACHY3M_002227"
     CDS             complement(27199..28572)
                     /gene="hemN"
                     /locus_tag="ACHY3M_002227"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFS4317140.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(28761..29270)
                     /gene="yihI"
                     /locus_tag="ACHY3M_002228"
     CDS             complement(28761..29270)
                     /gene="yihI"
                     /locus_tag="ACHY3M_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFS4317141.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(29389..29555)
                     /locus_tag="ACHY3M_002229"
                     /pseudo
     CDS             complement(29389..29555)
                     /locus_tag="ACHY3M_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29852..30484
                     /gene="yihA"
                     /locus_tag="ACHY3M_002230"
     CDS             29852..30484
                     /gene="yihA"
                     /locus_tag="ACHY3M_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4317142.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(30866..33652)
                     /gene="polA"
                     /locus_tag="ACHY3M_002231"
     CDS             complement(30866..33652)
                     /gene="polA"
                     /locus_tag="ACHY3M_002231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4317143.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            34016..34948
                     /gene="yihG"
                     /locus_tag="ACHY3M_002232"
     CDS             34016..34948
                     /gene="yihG"
                     /locus_tag="ACHY3M_002232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS4317144.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(34989..36418)
                     /gene="yihF"
                     /locus_tag="ACHY3M_002233"
                     /pseudo
     CDS             complement(34989..36418)
                     /gene="yihF"
                     /locus_tag="ACHY3M_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
BASE COUNT         8925 a         9326 c         8883 g         9349 t
ORIGIN      
        1 tttttgattg gatctttatt tgcataatct gaatggtgta cccgaaaaat gtcagcatcc
       61 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcca
      121 ccaaccggtc tttacttgtt catcgttata attttgttgg taacagttcg ggaaacaagg
      181 aattccttcc ggaacccccc ggtatcatcc gatagcattg acgtcaatca tcagcgcgac
      241 gatgagtttt tttaatcgca tcatagggtg gcgacgttca gcacgattac gggtcatagc
      301 aaaactcctg ggcaggcgac cactatgccg ctttcacagg agttctgaat gctagtgctt
      361 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
      421 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
      481 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
      541 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
      601 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
      661 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
      721 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
      781 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
      841 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
      901 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
      961 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     1021 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     1081 acgtctctgc cgggtttgtc tttagatttg cgaggcgctt tccagatttt tcacgcccgt
     1141 gaatttccct gtatccatcc agcaaaaacg ggtgtataag actgtacatt catacagcaa
     1201 aataagttct actatggcac tggaaaaagg aattgcagag ttagtcgaaa agtttatcgc
     1261 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     1321 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     1381 agggatgacc ttacgaatag tgtcgccgct gaatgccccg acattactcc cgacgataat
     1441 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     1501 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     1561 acatactttc cccgctgccc atgatgatgc gcaacgaggt gcagaaatag tctggcaaca
     1621 cgccgaacga ctcggcgttg ataaacaacg tataactctt gccggtgaca gtgcgggcgg
     1681 acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     1741 actcatctta atttatccta tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     1801 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtatctgcc
     1861 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     1921 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     1981 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     2041 ccacggattt tttcagttag gtggtatcag tgaggccgcg cgatcggcac tacgggatgt
     2101 tgcctggaga gcgggaaggt aacttcggac ctgggccctg aaaaggtttt cagattgaaa
     2161 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     2221 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     2281 cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     2341 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttaccgcta atcggcttgc
     2401 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gagatggcgt tcgcgcagca
     2461 tcagccagat aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca
     2521 gcgtatggtg cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg
     2581 tatattgttg aatacgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
     2641 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
     2701 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     2761 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
     2821 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtt cctaaccctt
     2881 tgtcctgcac gtccgggtga acggccataa agcgaatgga cgcttcattg tcggcattaa
     2941 tatacaatcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     3001 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     3061 tttcccagcg aaactgatag taacgctcta actcttcttc tgtttgtgga acccgaaggt
     3121 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     3181 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg cagcgaagcg
     3241 tcctgtttgc gtgttcatct cttgctggcg gcagcgttcg acgaaatagt catataacgc
     3301 ctctgcgcga tccggtgatg cgcctttaga gaaacttggg cgcatccccc gttcggtatc
     3361 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     3421 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     3481 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     3541 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     3601 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     3661 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     3721 acaaagagaa tggggatcac cgccaacacg ccgtaaatga gctgatatga cgggaacatg
     3781 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     3841 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     3901 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttatcgat gacagtattg
     3961 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     4021 agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc
     4081 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat cagcaataac
     4141 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     4201 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     4261 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     4321 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     4381 gtcatgttgt cctcatcaat acgttgccag agtagtttta gccaggccca tagtggacgg
     4441 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     4501 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgattg gctccttcta
     4561 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     4621 aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     4681 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     4741 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     4801 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     4861 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     4921 ccccacgctc atgctgatga aacgcctccc ccatgtggaa actcttctta agcgtagcca
     4981 gtggaacacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa
     5041 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     5101 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggttaacggt
     5161 cacaaaaatc tacgcgtctt cctggggaag aatgatttca atttggctgt cttgtaagcg
     5221 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt
     5281 accaatttca accgccgccg aagcatgata cttagtgtga tcggcgacca gcagaatatt
     5341 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     5401 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc
     5461 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     5521 gggcaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     5581 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacagtcgt
     5641 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     5701 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     5761 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     5821 aatatcccgg cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     5881 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     5941 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     6001 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgtgt
     6061 gcattttaac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacttgt
     6121 tgccagcgcc accgccaaag cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     6181 atcaactttg aaggccggtt gatgctgacg cccaccattt tccagccagt cgcagcctgc
     6241 gctaccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     6301 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc
     6361 gcttaatgcc atcagctcac tgatatcctg cggcgtaatg tccccgtcca gaacggtcat
     6421 cacacccgcc tgacgggcca gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     6481 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     6541 cagatccggg ctggggtagt taatgattat ccgctcgcct ttggtatcca ccatgatggc
     6601 ggattgcgaa gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga
     6661 ttccagctct gccagcaggc tgttgcccgt gtcgtcatca cctacgcgac caataaaatc
     6721 gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     6781 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     6841 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     6901 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     6961 tttgacgata aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca
     7021 aaaagtgaca tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag
     7081 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct
     7141 ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca
     7201 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     7261 tggtgcgcaa cgtgttgttc ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc
     7321 tggtcattga tatgtccacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     7381 aagccaaagg cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta
     7441 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacaccga
     7501 tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg gggatccgcg
     7561 ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgccg
     7621 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     7681 ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc ggagatcttt
     7741 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     7801 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     7861 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     7921 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     7981 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     8041 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     8101 cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat
     8161 tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga ttgccaaaag
     8221 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtcgatag
     8281 cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     8341 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa
     8401 agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc cagtcgtccg
     8461 cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     8521 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc
     8581 gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata tgccatgggt
     8641 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     8701 ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc
     8761 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat
     8821 cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg aaatggttta
     8881 acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt gattttggta
     8941 aaaattcagt agtgccgact ggttttggct ggttaggcaa taaagggcaa atcaaagaag
     9001 agatgggcac ccatctgtgg atcaccgctc gtatgttaca cgtttattcc gttgctgcgg
     9061 cgatgggtcg acctggcgct tactcgttgg ttgatcacgg tatcaaagcc atgaacggcg
     9121 cactgcgtga taaaaaatat ggcggctggt atgcctgcgt gaatgacgag ggcgtggtgg
     9181 atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc agcgccgtca
     9241 caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgaaatt atcgagaaat
     9301 atttctggag cgaagaagag cagatgtgcc tggaatcctg ggacgaagcc ttcagcaaaa
     9361 ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc ttgattgttt
     9421 atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta
     9481 tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat acccagtgga
     9541 atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg ttcggaggta
     9601 caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct gccctggaag
     9661 cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg tttaacgcca
     9721 ccgtgcgcga tgcctgggca cccgatggcg cggacgggat tgtttatacc gttgactggg
     9781 aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca atgggtacgg
     9841 cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat caaacatggt
     9901 gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg caggagctgg
     9961 atgcggacaa taaggtcacc accaaagtct gggacggcaa acaggatatt tatcacctgc
    10021 tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca gcggttgcag
    10081 cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg ggttttcccc
    10141 tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga
    10201 catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa
    10261 tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg cgaccattgt
    10321 gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag tcatcccgca
    10381 caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt ggccgaaccg
    10441 gatcgccaac ggctgttacc gctatcaggg gcaggaatat cagttaccca ttaatgaaca
    10501 tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa ttagtgaatt
    10561 aaccgccacc agcgttaccc tgacagcttt tctgccgccc agttacggct atccgtttat
    10621 gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg ttgagatcgc
    10681 aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcata cttatttaac
    10741 ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc aggtttatgc
    10801 cgttgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg atttaaattt
    10861 cacgcaggcg aaaacaatag ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga
    10921 tctgtgggaa ataaccatta cccatccaca acaggcgctt tcggttagcc tttgttgcga
    10981 tcaaccctgg gtacaaattt atagcggtga aaaattacag cgtcagggac tggcagttga
    11041 acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat tactggaacc
    11101 aggaaaaccg caccgcctgt tttttaacat ttacggacag agaaaataac ttgtattgat
    11161 tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat gtatcttcaa
    11221 tttattaatt taaaaacagc tcagcttctg taaagttttt atatatcagt tttatgagaa
    11281 tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa ctctatggag
    11341 attagttatg gatacgccac gtccacagtt attagatttt caatttcatc agaataacga
    11401 cagttttacc ctacattttc aacaacgtct tattttaacc catagcaaag ataatccttg
    11461 tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt tcagcattaa
    11521 agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt caccggatgg
    11581 ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata tctctgccga
    11641 cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca accgtatctg
    11701 gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac agttttccta
    11761 cttcgatctg cgtggcaaac cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa
    11821 caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcggacg gcgactatta
    11881 ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact gccatgttga
    11941 taacagttgc tatatgaact tcgactttag tgccccggaa taccatgaac tggcgctgtg
    12001 ggaagacaaa gcaacgctgc gttttgaatg tgctgacaca tacatttccc tgctggaaaa
    12061 attaaccgcc ctgctgggac gccagccaga actgcccgac tggatttatg acggagtaac
    12121 gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc
    12181 gggcgtgaag gtcaacggca tctgggcgca ggactggtcc ggtattcgta tgacctcttt
    12241 tggcaaacgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc aactggattc
    12301 acgcattaag cagtggaatc aggagggcgt gcagttcctg gcctatatca acccgtatgt
    12361 tgccagcgat aatgatctct gcgaagaagc ggcacaacac ggctatctgg caaaagatgc
    12421 ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg tcgatctcac
    12481 taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga ttgaactcgg
    12541 ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacactt acctgcataa
    12601 cggcatcagc gccgaaatta tgcataacgc ctggcccgcg ctgtgggcga aatgtaacta
    12661 cgaagccctt gaagaaacgg gcaagctcgg cgagatcctt ttctttatgc gcgccggttc
    12721 taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg tcgactggag
    12781 tctcgacgat ggcctggcgt cggttgtccc ggcggcgctg tcgctggcaa tgaccggaca
    12841 tggcctgcac cacagcgaca ttggcggtta caccaccctg tttgagatga agcgcagcaa
    12901 agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc gcacccacga
    12961 aggtaaccgt cctggcgaca actggcagtt tgacggcgac gcagaaacta tcgcccattt
    13021 tgcccgtatg acctccgtct tcaccaccct gaaaccttac ctgaaagagg ctgtcgcgct
    13081 gaatgcgaag tccggcctgc cggttatgcg cccgctgttc ctgcattacg aagacgatgc
    13141 gcacacttac accctgaaat atcagtacct gttaggtcgc gacattctgg tcgctccggt
    13201 gcatgaagaa ggccgtagcg actggacgct ctatctgccg gaggataact gggtccacgc
    13261 ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca tcggcaagcc
    13321 gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt taaaaagcat
    13381 ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga tgagtcacat
    13441 cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct cttacggtat
    13501 tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt tgaagtttta
    13561 taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga tttcaaaatt
    13621 ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac gcaagatcgg
    13681 tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca ccttactggc
    13741 gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga tggccactat
    13801 tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg gcgcaatggt
    13861 tcccgctatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg
    13921 cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagtgatga atcttatcga
    13981 gggtaatcag caacttggct atatcttcgc cgccacgctg ttttcactgt tcggcctgct
    14041 gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa
    14101 tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta accgcccact
    14161 gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca agctcgccat
    14221 tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt atatgggatt
    14281 tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag tcagacgttt
    14341 tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg atctgctcaa
    14401 ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg
    14461 ctcagcgttt gttaacagcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga
    14521 gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa
    14581 agtgtctcag gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt
    14641 gccgaacgtc gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta
    14701 cccaagcgca ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga
    14761 gaagatgtat gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata
    14821 ataaacgacg ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac
    14881 attaaaactg aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt
    14941 aatgctctgc atcggtactc tgtatctcct caaattttat accgatgaat tagggatgcc
    15001 tgcttactat ggcgggatta tctttctggt ggcgaagttt tttaccgcgt ttaccgatat
    15061 gctcaccgga tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc
    15121 ctttatttta tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac
    15181 caccttttgt ttgccggtta aaacgacgat tgccaccgcg ctgtttatga tgtttggact
    15241 ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc
    15301 gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat
    15361 ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc
    15421 ctgtgcggca cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg
    15481 cgtcaaagag cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc
    15541 tttttgcgcg atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac
    15601 tctggcggca tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa
    15661 tgatattaat ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt
    15721 attactggtg ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat
    15781 ggtgctatgg gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt
    15841 cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc
    15901 actggtacca gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc
    15961 tgtttatacc ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc
    16021 aggcattatg ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt
    16081 acagggtttg cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt
    16141 gacgatggga ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat
    16201 caaccaacgc aaaaataaag agatggcgac agaagaaaaa acggcttccg taacgttata
    16261 aatgtcaata gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg
    16321 gacttattat gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg
    16381 tatttgctgg cacatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg
    16441 aggttatgct acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata
    16501 cctacacctt tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt
    16561 atcctttatt caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata
    16621 agagtatcgg ttcgggtggt gcagtgtatc tggacgtcaa ctataaattt gtaccatggt
    16681 ttaatctgac agtacgaaat cgctataacc ataataacta tagttcaaca gatttgagcg
    16741 gggaactgga taataatgac acctatgaaa ttggcaccta ctggaatttt aaaatcaccg
    16801 ataaattttc ctatacattt gagccacatt atttcatacg cgtaaatgac tttaatagca
    16861 gcaatggaaa agatcatcac tgggaaatca ctaatacctt ccgttatcgc attaatgaac
    16921 actggctgcc ttatttcgaa ttacgttggt tagatcgtaa tgtcgaaccg taccaccgcg
    16981 agcaaaacca gattcgtatc gggacgaagt atttcttcta atgtgaaact gccggatgcg
    17041 gcgtaaacgc cttatccggc ctacggttga tacggttttc gttggccgga ttaagcacat
    17101 ccggccatta atcaagcttc agccgccagc atctctttct tacgttggct aattcgctta
    17161 accagcatta cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg
    17221 attttgtagc caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag
    17281 aacatcgccg gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt
    17341 tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc
    17401 gcgccaaagc ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc
    17461 ataatcaaca gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc
    17521 ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga
    17581 cagtattggt taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt
    17641 ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta
    17701 aaagcgataa gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat
    17761 gctccgtctt cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag
    17821 aaaataataa tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa
    17881 cctaataagc cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta
    17941 tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg
    18001 cccaatcgac ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca
    18061 aatagcgccc agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc
    18121 agtaatcctg tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca
    18181 aaataaatag acagaaaaaa gcccactgtg gtgacaaatg agtttaccga catagcatta
    18241 ccaatttgac cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc
    18301 agcgacggta atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat
    18361 ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa
    18421 taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt
    18481 agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat
    18541 ttatctgatt aattgcatct tctttttctt tacgtaaccg cacatccagt aaatgatttt
    18601 ctggcaatgg tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt
    18661 caaagcggaa cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct
    18721 gcggctcatc atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac
    18781 cctgacctga aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat
    18841 ctttgatatg gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct
    18901 catttgcatt gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt
    18961 ctttgaccag cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag
    19021 tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata
    19081 atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt
    19141 tcaacgcaat ggcgacggct tcgtcaatag atgccttatc tgaagcgctg gtttcaatat
    19201 gaatatcaag gttcagacgg cgggctttat ccccaaaggc agagagttct ttatcgtcca
    19261 tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat
    19321 tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat
    19381 gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg
    19441 cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat
    19501 cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca
    19561 attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctaatatc gcccggcatt
    19621 ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag
    19681 actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta
    19741 atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga
    19801 tagagttcag ttgatggtgt cactgcaatc agtggcgcga gcgtcttggg aatatcgctg
    19861 gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc
    19921 tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc
    19981 ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc
    20041 gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc
    20101 ggcgtggtga tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg
    20161 gattgattct ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct
    20221 tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc
    20281 tctattgacg taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca
    20341 ggtaaaaaaa acctgccagc gatggcaggt tttttataac taaaaaacga aattaatcgt
    20401 ctttcggtgc gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac
    20461 ggatagaggt cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt
    20521 ccagagtcat gcggataggc ggaaccagaa caacggcttc gtcagtaccg gaagcacgca
    20581 tgttggtcag tttcttaccg gtcaggcagt ttaccgtcag gtcgttagag cggctatgaa
    20641 taccgataat ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat
    20701 cctgcagacc gaacagcgcg aacgcgaccg ctttaccctg accgttagag atcagtacgc
    20761 cgttctgacg ctgacccact tcacccggac gtacgtcgtc gtagtggctg aaggtggagt
    20821 acagcagacc agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac
    20881 ggcttgggat cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca
    20941 ggtcgccttt acgttcgccc agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt
    21001 ccagagtcac gttttcatac ggctcttgtt tacgaccgtc gatttcacgg aagataactt
    21061 tcggacggga taccgccagt tcgaaacctt cacgacgcat gttttcgatc agaacagaca
    21121 ggtgcagttc gccacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc
    21181 gcagcgcaac gttgtgtacc agttctttgt tcagacgatc caggatctga cgagacgtta
    21241 cgaacttacc ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg
    21301 tcggctcatc aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt
    21361 cagaaatgtt cagttcgcca aggcccgtga tcgcaacgat atcgccagct tccgccagat
    21421 cggtttcgat acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttgcggg
    21481 ttttgccttc gctatcgatg atagtgacct gctggttcgg cttcacttta ccgcgcttga
    21541 tgcggccaat gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct
    21601 ggaacggacc gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctggtaca
    21661 gcggggtcat gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag
    21721 aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat
    21781 cgaatacctg atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa
    21841 caacaatcgg cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca
    21901 tcgggccgtc aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt
    21961 caacttcacc accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat
    22021 tccatttgat agcggtgttt ttcgcgagga tggtaatccc ccgctctttc tccaaatcgt
    22081 tggagtccat cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga
    22141 gcagcttgtc taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac
    22201 gcaatttttc gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga
    22261 ttaatcgcac tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc
    22321 tttgtgatcg ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaacatgg
    22381 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tcacatcgtg gtgcagccct
    22441 tttgcacgat ggtgcgcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt
    22501 tcgctttatc ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg
    22561 accatgacca atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa
    22621 cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat actaaaggta aagaacagca
    22681 cgtcactatc cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga
    22741 cggctcctcg attggcggct ggaaaggcat taacgagtcc gacatggtgc tgatgccaga
    22801 cgcatccacc gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga
    22861 catccttgaa cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg
    22921 cgccgaagat tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc
    22981 tgaattcttc ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc
    23041 tatcgacgat atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg
    23101 tcaccgtccg gcagtgaaag gcggttactt cccggttcca ccggtagact cggctcagga
    23161 tattcgttct gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca
    23221 ccacgaagta gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa
    23281 aaaagctgac gaaattcaga tctacaaata tgttgtgcac aacgtagcgc accgcttcgg
    23341 taaaaccgcg acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg
    23401 ccacatgtct ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct
    23461 gtctgagcag gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc
    23521 cctggcaaac ccgaccacca actcttataa gcgtctggtc ccgggctatg aagcaccggt
    23581 aatgctggct tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc
    23641 tccgaaagca cgtcgtatcg aagtacgttt cccggatccg gcagctaacc cgtacctgtg
    23701 ctttgctgcc ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga
    23761 agccatggac aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt
    23821 tgcaggctct ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc
    23881 cggtggcgtg ttcactgacg aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga
    23941 tgaccgcgtg cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata
    24001 gttgaagttg tactacccgg cgcaacaacg ccggaggttc aaaccaggcc catctggcgt
    24061 aattgttgca gccagtttga acacggacag cgcgcagaac ccggagcgta cactggtacg
    24121 tgaggagttc gagcactgcc caggttcaaa atggcaaata aaatagcctg atgggactgg
    24181 tttttagttg ccgtggaaat ttcagcccat ctctgcatgg gcttttttct ccgtcaattc
    24241 tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg
    24301 ttcaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc
    24361 gctgattaac agtattttgt taatcgatga caacctggcg atccattacg ccaaccctgc
    24421 cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact
    24481 gttgagctac ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg
    24541 ttttaccgat aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac
    24601 ggcccagcgt atgccggacg gcatgatcct gctggagatg gctccgatgg ataaccagcg
    24661 ccgcttaagt caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg
    24721 cggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctgct
    24781 cagcaaagcg ttacctgacc catcactact cgaatatacc aaagtgatta tcgaacaggc
    24841 ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt
    24901 taccgaaagt attcacaaag tggctgaacg cgtggtaacg ctggtgtcga tggaactgcc
    24961 ggacaacgtg cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc
    25021 ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc
    25081 ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga
    25141 gcgctaccgg ctggcggcgc ggattgatgt ggaagataac gggccgggca ttccgcctca
    25201 tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg
    25261 cttatccatc gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg
    25321 gccagggcat accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg
    25381 caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt
    25441 gcgctcgctg gggcaggttt aacctgtacg acgtttgaga acggcgcaga agtgctggag
    25501 gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac
    25561 gggctggcgc tgctcaagca gattaaacag cgccatccaa tgcttccggt catcattatg
    25621 accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gtttgattat
    25681 ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg ttgagcgcgc tatcagtcat
    25741 taccaggaac agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc
    25801 ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct
    25861 attagcgtgc tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg
    25921 catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc tgaatatggc agctatccca
    25981 aaagatttga tcgaatcaga actgtttggc cacgagaaag gcgcgtttac tggcgcgaat
    26041 accattcgtc aggggcgttt tgaacaggcc gatggcggta cattattcct cgacgaaatt
    26101 ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt
    26161 taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac
    26221 cagaatctcg aacagcgagt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg
    26281 aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc gggaagatat tccccgtctg
    26341 gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg tagaagcgaa gttactgcat
    26401 ccggaaaccg aagctgctct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa
    26461 aacacctgcc gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg
    26521 cccggcgaac tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacaac
    26581 tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc gttccggtca tcaaaatctg
    26641 ctttccgaag cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg
    26701 caggggcata aacaggaagc ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt
    26761 aagttaaaag agctggggat ggagtgattc acagcttgtg tgtaaagatt gattattgag
    26821 cgcaaattgc tggtatttta cgctttactg ttccgataag ttcagtatga tcttgcccgg
    26881 aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt
    26941 gacagccaca caccgcaaac tgccgttgct gccgtgctgt gtgccgcaat gattgggctg
    27001 tttagttgag tttcagattg ctgatgaagt gctctttgtt catgccggat gcggcgtgaa
    27061 cgccttatcc ggcctacaaa agcaatattt tcaacaggtt ttgtaggcct gataagcgta
    27121 gcgcatcagg caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa
    27181 cgaagcgcca ttcactattt aaatcacccg tgagaactgc tgcatccgcg ctttctggcg
    27241 cagataggta tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac
    27301 ctgtattccc ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt
    27361 gagatcttcc gcaaagtaat cagcgaagtg caaatcccac tgtttctcaa taggggcgta
    27421 atccagacgg aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg
    27481 cgttagcgca ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg
    27541 cttcaactct ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc
    27601 catccccagc agatcggtat cgccctgagt ggtgtagccc tggaagttac gatgcagcac
    27661 gccttcacgc tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat
    27721 aaactgatag cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg
    27781 ctgcggactc ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag
    27841 atgcgcgtag ttaaagacac tcagacgatc ggggttcagc tccgccacac gtttcagggt
    27901 aaaggcgaaa ctctccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga
    27961 ggtaaaacca atctcacgcg catggttaag cagtgcaaag atgaactctt catcctgctc
    28021 gcggttaacc agacgttgca cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt
    28081 aaagccttcg gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc
    28141 gatcgaaatc tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcaggcg
    28201 gctgatttgc gctttattca gatacgtcgg cgttccgccg ccccagtgca attggctgac
    28261 gtgacgcccg gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata
    28321 ctgatcggcc ttgtgctgct ggcgagtaac aatcttattg caaccgcaga agtaacaaag
    28381 cttatggcag aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac
    28441 ggcttgtaaa aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga
    28501 ggtgtatcgt ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg
    28561 ctgtacagac atgctcactc cttccaatgg cgtctctggc ggctacggcg taccggcgga
    28621 tgcccgtttc gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt
    28681 agtttaacga ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga
    28741 aggcccacgg cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc
    28801 tcgtcttctt cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca
    28861 tcaatacgat ccagtttagc atccacccag gattgctctt cggcgctcag ggtttcgcct
    28921 gcttccagac gttccagcag cgcatccaga cgctcatccg tttccagtaa ttccaactcc
    28981 gcctgcggtg aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca
    29041 gtcacgccca atggaatagg ggttttactg ccaatacgtg gatcttttgg tgcgttctgg
    29101 cctttgctgc ctgacgtggt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga
    29161 cgttttttct gacgcttgcg atcacgagct tcctgatcca gctcctcgcg tgtttttcga
    29221 cgcgcttttg catgaccttt gctgcgtgag tttgaagatg atggtttcat aatggttcgt
    29281 cttttagccg ttttattcag tatagatttg cggcggaatc taacagaaag caagcaaaga
    29341 aaaaaggcga cagattactc tgtcgccttt tttcctgact cataaccctt aacgggtctt
    29401 acaatccttg caggccgcca acacaccctg tttttccttt agccggaaca atccgtgttg
    29461 attccatttc cgtttaatta cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc
    29521 gcctcctggc gctcctgacc ctcatcctga gtcattgttc ctgttagcgt cctcgctcta
    29581 tgggtgctac tttacgcctt tgctttaaac aaacaatcaa ccgccatcag tatttgcaca
    29641 ttttcagagt tttatataaa agcaattcac tgatttatat aacttatttg acatgcatcg
    29701 cagaattttc tctgctattt cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc
    29761 ttttacttac aacgctcacg ccgaaaaatc cgccttttat tatggttcag gtataatcgc
    29821 cacaaagctt tgactgacgg agacgaccgc tttgactaat ttgaattatc aacagacgca
    29881 ttttgtgatg agtgcgcctg atattcgcca cctaccttcc gataccggaa ttgaagtggc
    29941 ttttgcaggc cgttccaacg caggtaaatc cagcgcgctg aacacgctga ctaaccagaa
    30001 aagcctggct cgtacctcaa aaaccccagg gcgcacccag cttatcaacc tgtttgaagt
    30061 ggctgacggc aagcgtctgg ttgacttgcc tgggtacggt tatgcggaag tcccggaaga
    30121 gatgaagcgc aaatggcagc gtgcgctcgg cgaatacctc gaaaaacgtc agagcctgca
    30181 aggtctggtg gtactaatgg atattcgcca tccgctgaaa gatttggatc agcagatgat
    30241 tgagtgggcg gtagacagca atatcgccgt tctggtgctg ctgaccaaag cggacaaact
    30301 ggcaagcggc gcacgtaaag cgcaattgaa tatggtgcgt gaagctgtac tggcgtttaa
    30361 cggtgatgtg caggttgaaa cgttttcttc gttgaagaaa caaggcgtgg acaagctgcg
    30421 gcagaaactg gatacctggt ttagcgagat gcagcctgta gaagaaacgc aggacggcga
    30481 ataattttct tgccttaatg cttatgccgg atgtggcgta tccggcccgt aaattcaata
    30541 gattacaatt ctaccgttgg cctgataaac atagcgcatc aggcattacg gatcttttct
    30601 ttcgcccaat aaaaaacgcc ccagtcatta ctgactgggg cggctaaaat attcagccaa
    30661 atccgattac gtgaagtaaa aggtctgaaa gatagaacat cttacctctg taccctacgc
    30721 gactaacttt actctttttt gttcagttca gaaagcactt tttgtaattt tttttcagtt
    30781 gttgcatagg aatttctaat agccatcaca aaacgtgaca gcttatgttg cttacttacg
    30841 aaaaaagggc atgttcaggc gaatcttagt gcgcctgatc ccagttttcg ccactcccca
    30901 cttccaccag caacggcaca tccagacggg tacagttttc catcagttga tgaatctgct
    30961 tcgcgacggc atcaacatca tctttatgaa cttcaaatac cagttcatcg tgtacctgca
    31021 tgatcatacg tacacgcggt tgctcagcct gtaaccacgc atcaacggca atcatcgccc
    31081 gtttgataat gtcggcggcg gttccctgca ttggcgcgtt aatggctgca cgttcagccg
    31141 ctgcacgacg agcaccattg ctggatttga tatccggcag atacagacgg cgtccgtcca
    31201 gcgtttcaac gtagccctgc tctttcgcct gagcacgggt gcgttccata tactccagca
    31261 cgccagggta gcgttcgaag taaaggtcca tgtacttctg cgcttcttta cgtggaatgt
    31321 tcaattgccg cgccagaccg aaagcactca tgccataaat cagaccaaag ttgatcgctt
    31381 tcgcgctacg gcgttgctcg ctggtgacgg tttccagtgg caaaccaaac acttctgccg
    31441 ccgttgcccg gtggatatct tttccttccg cgaatgcggt cagcaagcct ttgtcacgcg
    31501 aaagatgcgc cataatgcgc agttcaatct gcgagtagtc cgctgagaca atcacataat
    31561 cctctggcgc aataaacgcc tggcggatac gacgaccttc ttcgttacgc accggaatgt
    31621 tttgcaggtt aggatcggtt gacgataaac gtcccgttgc agttactgcc tggtgataag
    31681 aggtatgcac acgcccggtt ttcgggttga tcatcagcgg cagcttgtcg gtgtaggtcg
    31741 atttcagctt cgccagacca cgatactcca gaatcacttt tggcaacgga tagtccagcg
    31801 ccagttcttc cagtacctct tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg
    31861 gtttaatacc ctgtttttca aagagaatgg tttgcaactg cttggtggaa gaaaggttaa
    31921 attcttcacc tgcaatttca tgcgctttct tttccagctc agccagacgc aaggtgagct
    31981 cttcagaatg attgtgcagc actttcggat cgatcttcac accgttacgt tcaatgcgtg
    32041 aaagcaccgg caccagcggc atttcgatat tctcgaagac gttcaacggc cctttgtgtt
    32101 tttgcagatc tggccacatt ttcagatgca actgcaaggt gacatcagca tcttcggcgg
    32161 cgtaacgtcc ggcctcttcg agggcaatct ggttaaaggt cagttgattt ttgcctttac
    32221 ccgcaatctc ttcaaaagtg atggttttgt gcttcaacca acgttccgcg aggctgtcca
    32281 tatcgtgacg cccggcaacg ctattgagaa tgtaggactc cagcatggta tcaaacgcaa
    32341 tcccacgcag ctcaatgccg taattcgcca gaataccgcg atcgtatttc aggttttgcc
    32401 cgaccttcag cgccttttca tcttccagca gcggttttag caactcgagt gcacgctcgc
    32461 gagagatttg atcgggcgcg tcaagataat catgagcaac cggaatatat gccgctacgc
    32521 ctggctcgat agcaaaagaa agcccgacca ggttagcaga gatgttatca aggctgtcgg
    32581 tttcggtatc aaatgcaaat accggcgctt tttccagctt cgcaatccac gctttcagtg
    32641 tttcttcatc aaggatggtg acgtagttgt cataagaaat caccgttgcc gtcacttctg
    32701 gcgcttcgtc tgcaacactg gtttcctgcg gcttcgcggc tggttttgcc cctttggcct
    32761 gtaaccattt gcccgcttcg acatcagcag tccagcgttt gaactcatac tttttgaata
    32821 gccccaacaa ctcttccgct gccggttgct gcacttccag ttgttcacag gtcagctcca
    32881 gttcaacgtc ggttttaatc gtcgccagct ggtatgagag ataagcaact tctttgtttt
    32941 gctcgagctt cgctgccatt gttttcgcgc cacggaagct caacccagca attttttctg
    33001 gctcggcata cagcgtatcc agcccgccaa gaccttgcag caatgcctgc gcggtttttt
    33061 caccgacgcc cggtacgcca ggaatgttat cggaggagtc acccatcagc gccaggaaat
    33121 cgatgatcag ttctggcggc acgccgtact tattcaccac ctcttccggt ccgaggatgg
    33181 tattcgtcat ggtattgata agcgtaatat ttggcgtcac cagctgcgcc atatctttat
    33241 cgccagtgct gatcagcacc ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga
    33301 taacgtcgtc cgcttctacg ccagaaaccg ccagcagcgg cagtcccatc gctttaacca
    33361 tcgcgtgcag gggttcgatt tgtgctcgca gatcgttcgg cattggcggg cgatgtgatt
    33421 tgtaatgttc aaacagttca tcacgaaagg tttttccctt ggcgtcaaag accaccgctg
    33481 catgcgtcgg tttatattgc atgatcagac tgcgcagcat gttgaggaca ccatacatcg
    33541 caccggtcgg ctcgcctgcg ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa
    33601 gataagatga accatctaca aggataagtg gattttgggg gatctgaacc ataatgtccg
    33661 tgcctgttta tcagattatg ggtaaaggat gccacagaca ggatgaaaac atgagttttt
    33721 caccgctttt cgtgaaaatc ttttgtagat cttctggatc gctcgcaaat cgtcatgtgg
    33781 ataactttgt gcataaatta tttacatcgc ttttaattta ttatcatgct ttttacagca
    33841 tgatatttgt ctttgttttt catgcagtta aaaataaagc catccctaat aattgagata
    33901 atgggatttt ttctaatgtg gatagatatg aattattttt ctccttaagg atcatccgtt
    33961 atttgggtcg ttttctgcta aaatctgcgc cttgcgcttc tttagcgcac tgtctatggc
    34021 taaccttttg aataaattca ttatgacgag aatactcgct gcgataaccc ttttgctgag
    34081 tatcgtattg accattttgg tcactatttt ttgttccgtc ccgatcatca ttgccgggat
    34141 tgtaaaacta ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt
    34201 tatgatgtat tgctggtgtg aaggtctggc ggtattactg catctgaacc cacacctgca
    34261 atgggaagtt cacgggctgg aagggttaag taagaagaac tggtatctgc ttatttgtaa
    34321 ccaccgtagt tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat
    34381 gaataaatat ttcctcaagc aacagctggc ctgggtgcca tttcttggcc tggcgtgctg
    34441 ggcgctggat atgccgttta tgaagcgtta ttcccgcgct tatttattac gtcatccgga
    34501 gcgacgtggt aaagatgttg aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc
    34561 caccaccatt gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac
    34621 ccactccact tttcaaaact tgttgccacc aaaggcggca ggcattgcga tggcgctaaa
    34681 tgtactgggt aaacaattcg ataaactgct taatgttacg ctgtgttatc cggacaataa
    34741 ccgtcagccg ttcttcgata tgttaagcgg taaactgacg cgaattgtcg ttcatgtgga
    34801 tttacagcct attgccgatg agttacatgg cgactacatc aatgacaaaa gctttaagcg
    34861 ccattttcag caatggctga actcactgtg gcaggaaaaa gaccaacttc tgacgtcgtt
    34921 gatgtcatca cagcggcagg acaagtaaat tttgctcgcc tctgaatgag cagaggcgag
    34981 tgagtatttt aatgaacaaa acgtccggca cgagacataa attcttcttc gctcatctcc
    35041 tgtccgttaa aaacaacttt acccggtgta taacgcagtt gcagcgaggc ggtattgttg
    35101 tcaatcgtga ttaactgaaa catctgacct aaggtcatca tcccgctgat ttgtttatca
    35161 gcccgctttt gcgctttttc cgcatccatt ccttcagata aattaagctg ttttgcggtt
    35221 tctgtgacga cattaagcgg taacttcaca tcaaaattga gcgatttaat atctttgttt
    35281 gtggatgatg aagatttggc tgggtcggca atactgatat ccagattggc atttagttcg
    35341 ccgagtgcgt tcttccattt taccggttgt ttaatggtcg gctcactttt ttgtaataac
    35401 ggtaaatact ctttgaacaa tgcagcattc acttcttgca gagcaacttc atcgtttgct
    35461 aactcagggt gtgcagcaag ctgcttctgc attgcaatgt tatactggat gataaattgg
    35521 cgcaccgctg agggatcaat agattcagca atcaatgacc actcaccact accgagttgc
    35581 tgattccctt ttgttaactt atcaagttcg taggtcagat tgacattgat ataatctttg
    35641 tctttaacgc gatccagcat ggtttttgca acgaaagcat cgatttgggc aacgtcttcc
    35701 ccgtgattga taatgttaat ctgattaaat ttcgactcgc tttcaccaac cggaaatttc
    35761 ttttcttcta atcgcgccag agaatcaatg gtaaaacttt ttgccgttac tttgttcatt
    35821 ggtgataatt gcagaacaat atcttcgact ttgccttcaa tatccaggtt agacaatgaa
    35881 ctaccatcac cattaaaagt aaactggccc tcaccaaaag aaaactttcc atactctgca
    35941 ggggcaacat ctaatgtcgt agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc
    36001 acgggcgatt tatttttcgc ggcgataaat agcggttgtg tcagttcatt attcactaag
    36061 ttcacttttg ccgctgccag tgctgggatg acattaccat gcattaacat tgtgatgggc
    36121 aacggaccgt gctcaacatc cacatcaaat acaactttct ggcctggctt gatattgaga
    36181 tcgggtgcac cgttatcgaa tgtcatttgc atttgaaaac gggaattaaa aaagcctttg
    36241 tcaaagtttt tataacttaa cgtcatatcg taggcatgtt cacctttctt tttcgcatcg
    36301 ttaaaatctt taataaactt ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg
    36361 ccaccaccca gattacggct aacgcaacaa taacacctgt agctgactta cgtatcatat
    36421 aaatattccc tgataaaaca gcaaaacttt gaatatccac ttatgctgaa ataatgtcca
    36481 gcg
//
LOCUS       DAZUFB010000040        36256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZUFB010000040 DAZUFB010000000
VERSION     DAZUFB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.040"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            310..645
                     /gene="yjdM"
                     /locus_tag="ACHY3M_002234"
     CDS             310..645
                     /gene="yjdM"
                     /locus_tag="ACHY3M_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4317145.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            805..1248
                     /gene="yjdN"
                     /locus_tag="ACHY3M_002235"
     CDS             805..1248
                     /gene="yjdN"
                     /locus_tag="ACHY3M_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFS4317146.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1381..2169
                     /gene="phnC"
                     /locus_tag="ACHY3M_002236"
     CDS             1381..2169
                     /gene="phnC"
                     /locus_tag="ACHY3M_002236"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS4317147.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIIALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2194..3210
                     /gene="phnD"
                     /locus_tag="ACHY3M_002237"
     CDS             2194..3210
                     /gene="phnD"
                     /locus_tag="ACHY3M_002237"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4317148.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3316..4095
                     /gene="phnE"
                     /locus_tag="ACHY3M_002238"
     CDS             3316..4095
                     /gene="phnE"
                     /locus_tag="ACHY3M_002238"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4317149.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4116..4841
                     /gene="phnF"
                     /locus_tag="ACHY3M_002239"
     CDS             4116..4841
                     /gene="phnF"
                     /locus_tag="ACHY3M_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFS4317150.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4842..5294
                     /gene="phnG"
                     /locus_tag="ACHY3M_002240"
     CDS             4842..5294
                     /gene="phnG"
                     /locus_tag="ACHY3M_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFS4317151.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5291..5875
                     /gene="phnH"
                     /locus_tag="ACHY3M_002241"
     CDS             5291..5875
                     /gene="phnH"
                     /locus_tag="ACHY3M_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFS4317152.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNALLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5875..6939
                     /gene="phnI"
                     /locus_tag="ACHY3M_002242"
     CDS             5875..6939
                     /gene="phnI"
                     /locus_tag="ACHY3M_002242"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFS4317153.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6932..7777
                     /gene="phnJ"
                     /locus_tag="ACHY3M_002243"
     CDS             6932..7777
                     /gene="phnJ"
                     /locus_tag="ACHY3M_002243"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFS4317154.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7774..8532
                     /gene="phnK"
                     /locus_tag="ACHY3M_002244"
     CDS             7774..8532
                     /gene="phnK"
                     /locus_tag="ACHY3M_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFS4317155.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8643..9323
                     /gene="phnL"
                     /locus_tag="ACHY3M_002245"
     CDS             8643..9323
                     /gene="phnL"
                     /locus_tag="ACHY3M_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFS4317156.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9320..10456
                     /gene="phnM"
                     /locus_tag="ACHY3M_002246"
     CDS             9320..10456
                     /gene="phnM"
                     /locus_tag="ACHY3M_002246"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFS4317157.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10456..11013
                     /gene="phnN"
                     /locus_tag="ACHY3M_002247"
     CDS             10456..11013
                     /gene="phnN"
                     /locus_tag="ACHY3M_002247"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFS4317158.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKKKHHACL"
     gene            11000..11434
                     /gene="phnO"
                     /locus_tag="ACHY3M_002248"
     CDS             11000..11434
                     /gene="phnO"
                     /locus_tag="ACHY3M_002248"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFS4317159.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11436..12194
                     /gene="phnP"
                     /locus_tag="ACHY3M_002249"
     CDS             11436..12194
                     /gene="phnP"
                     /locus_tag="ACHY3M_002249"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFS4317160.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12341..12670)
                     /gene="yjdP"
                     /locus_tag="ACHY3M_002250"
     CDS             complement(12341..12670)
                     /gene="yjdP"
                     /locus_tag="ACHY3M_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFS4317161.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12739..13056)
                     /locus_tag="ACHY3M_002251"
     CDS             complement(12739..13056)
                     /locus_tag="ACHY3M_002251"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFS4317162.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13309..15294
                     /gene="yjcS"
                     /locus_tag="ACHY3M_002252"
     CDS             13309..15294
                     /gene="yjcS"
                     /locus_tag="ACHY3M_002252"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFS4317163.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            15503..15778
                     /gene="ytcA"
                     /locus_tag="ACHY3M_002253"
     CDS             15503..15778
                     /gene="ytcA"
                     /locus_tag="ACHY3M_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFS4317164.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            15797..16828
                     /gene="mdtN"
                     /locus_tag="ACHY3M_002254"
     CDS             15797..16828
                     /gene="mdtN"
                     /locus_tag="ACHY3M_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFS4317165.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVMPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            16828..18879
                     /gene="mdtO"
                     /locus_tag="ACHY3M_002255"
     CDS             16828..18879
                     /gene="mdtO"
                     /locus_tag="ACHY3M_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFS4317166.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDRRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            18876..20342
                     /gene="mdtP"
                     /locus_tag="ACHY3M_002256"
     CDS             18876..20342
                     /gene="mdtP"
                     /locus_tag="ACHY3M_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFS4317167.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTSIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            20540..22687
                     /gene="fdhF"
                     /locus_tag="ACHY3M_002257"
     CDS             20540..22687
                     /gene="fdhF"
                     /locus_tag="ACHY3M_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:20957..20959,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFS4317168.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGLQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            22781..23470
                     /gene="yjcO"
                     /locus_tag="ACHY3M_002258"
     CDS             22781..23470
                     /gene="yjcO"
                     /locus_tag="ACHY3M_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFS4317169.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(23572..24861)
                     /gene="gltP"
                     /locus_tag="ACHY3M_002259"
     CDS             complement(23572..24861)
                     /gene="gltP"
                     /locus_tag="ACHY3M_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFS4317170.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLG"
     gene            complement(25203..25799)
                     /gene="nrfG"
                     /locus_tag="ACHY3M_002260"
     CDS             complement(25203..25799)
                     /gene="nrfG"
                     /locus_tag="ACHY3M_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFS4317171.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(25796..26179)
                     /gene="nrfF"
                     /locus_tag="ACHY3M_002261"
     CDS             complement(25796..26179)
                     /gene="nrfF"
                     /locus_tag="ACHY3M_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFS4317172.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(26172..27890)
                     /gene="nrfE"
                     /locus_tag="ACHY3M_002262"
     CDS             complement(26172..27890)
                     /gene="nrfE"
                     /locus_tag="ACHY3M_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFS4317173.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(27910..28866)
                     /gene="nrfD"
                     /locus_tag="ACHY3M_002263"
     CDS             complement(27910..28866)
                     /gene="nrfD"
                     /locus_tag="ACHY3M_002263"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFS4317174.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(28863..29534)
                     /gene="nrfC"
                     /locus_tag="ACHY3M_002264"
     CDS             complement(28863..29534)
                     /gene="nrfC"
                     /locus_tag="ACHY3M_002264"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFS4317175.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(29531..30097)
                     /gene="nrfB"
                     /locus_tag="ACHY3M_002265"
     CDS             complement(29531..30097)
                     /gene="nrfB"
                     /locus_tag="ACHY3M_002265"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFS4317176.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(30142..31578)
                     /gene="nrfA"
                     /locus_tag="ACHY3M_002266"
     CDS             complement(30142..31578)
                     /gene="nrfA"
                     /locus_tag="ACHY3M_002266"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFS4317177.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            31970..33928
                     /gene="acs"
                     /locus_tag="ACHY3M_002267"
     CDS             31970..33928
                     /gene="acs"
                     /locus_tag="ACHY3M_002267"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFS4317178.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            34129..34443
                     /gene="yjcH"
                     /locus_tag="ACHY3M_002268"
     CDS             34129..34443
                     /gene="yjcH"
                     /locus_tag="ACHY3M_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFS4317179.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            34440..36089
                     /gene="actP"
                     /locus_tag="ACHY3M_002269"
     CDS             34440..36089
                     /gene="actP"
                     /locus_tag="ACHY3M_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFS4317180.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         8450 a        10231 c         9731 g         7844 t
ORIGIN      
        1 aattttatat ttagcgtgtt gttttttatt ggaataaata acgtcttccg atacgtttaa
       61 aacgtcagaa acataaaaat atcatgtgaa ttaaaaaaaa gaacaagcag agcattaaca
      121 ttatcttaaa taataaatgg aggcaaaaag attattttct gtttgcgttt cctttcaaat
      181 gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa actcagggat tttgtgtata
      241 attgcggcct ttttcggcaa tctgccgttt tttggcgctt ttgccctgct gacttttgag
      301 gaaatccaca tgtcattacc acactgccca aaatgcaact ccgaatacac ttacgaagat
      361 aacggcatgt acatctgccc ggaatgtgcc tacgaatgga acgacgcaga acctgcacag
      421 gaaagcgacg agctgatcgt taaagatgct aacggcaatc tgctggctga cggcgacagc
      481 gttaccatca ttaaagatct gaaggtgaaa ggtagctctt cgatgctgaa aattggcacc
      541 aaagtgaaaa acatccgcct ggttgaaggc gaccataaca tcgattgcaa aatcgacggt
      601 tttggtccga tgaaactgaa atctgagttt gtgaaaaaga actgattgta ttgtgatcgg
      661 taagccggat aaggcgctcg cgccgcatcc ggcaacgatg ccgactgcct gatgcgacgc
      721 tgaccgcgtc ttatcaggct tccctctcat ctgtataaat ttcgaactac acttaactgg
      781 cttctcttaa ctgaggtcac catcatgccg ttaagtccct acctctcttt tgccggtaac
      841 tgtgccgacg caattgctta ttatcaacgt acgttgggcg ctgaactgct ctataaaatc
      901 agcttcggcg aaatgccaaa atcagcgcag gacagcgccg agaactgccc ttccggaatg
      961 caatttcccg ataccgccat cgcccacgcc aacgtgcgca ttgccggaag cgacatcatg
     1021 atgagcgatg ctataccgtc aggaaaagcc agctactccg gctttacgct ggtgctcgat
     1081 tcgcaacagg tcgaagaagg aaaacgctgg tttgacaatc ttgccgctaa cggaaaaatc
     1141 gaaatggcct ggcaggaaac tttctgggcg catggctttg gcaaagtcac cgataaattt
     1201 ggcgtaccgt ggatgattaa tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg
     1261 cccgccctcc cgcactgtca tcgaattccc gttaactctt catctgttag tcacttttaa
     1321 ttaaccaaat cgtcacaata atccgccacg atggagccac ttttttaggg aggctgcatc
     1381 atgcaaacga ttatccgtgt cgagaagctc gccaaaacct tcaatcagca tcaggcgctg
     1441 catgcggttg atctgaacat tcatcacggt gaaatggtgg ctctgcttgg gccgtcgggt
     1501 tccggcaaat ccaccctttt acgtcactta agcggtttga ttaccggcga taaatccgcc
     1561 ggcagccata tcgagctgct gggccgcaca gtccagcgcg aaggccgtct ggcgcgcgat
     1621 atccgcaaaa gccgcgccaa caccggctac atcttccaac aattcaacct ggtgaaccgc
     1681 ctgagcgtac tggagaacgt gctgattggc gcgctcggca gcacgccgtt ctggcgcacc
     1741 tgttttagct ggttcaccgg cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt
     1801 ggcatggtgc attttgccca tcagcgcgtt tccaccctct ccggcggaca gcagcagcgt
     1861 gtggcgattg cccgcgcgct gatgcagcag gcgaaggtga ttctggccga tgaacccatc
     1921 gcctcgctgg acccggaatc cgcccgcatc gtgatggaca ccctgcgcga catcaatcag
     1981 aacgacggca tcaccgtggt cgtcacgctg catcaggtgg attacgccct gcgctactgc
     2041 gaacgcatca tcgccctgcg ccaggggcac gttttctacg acggcagcag ccaacagttt
     2101 gataacgaac gttttgacca tctctaccgc agcattaatc gcatcgaaga gaacgcgaaa
     2161 gctgcctgac atccccatca ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg
     2221 gccttcacca gcatgttcag cctcagcacc ctgttaagcc cggcacacgc cgaagagcag
     2281 gaaaaggcgc tgaatttcgg cattatttca acggaatcac agcaaaacct gaaaccgcaa
     2341 tggacgccat tcttacagga tatggagaag aagctgggcg tgaaggtgaa cgccttcttt
     2401 gccccagact acgcaggcat tatccaggga atgcgcttca ataaagtgga tatcgcctgg
     2461 tacggcaacc tgtcggcaat ggaagcggtg gatcgcgcca acggccaggt cttcgcccag
     2521 acggtcgcgg cggatggatc gccaggttac tggagcgtgt tgatcgtcaa caaagatagt
     2581 ccgatcaaca acctgaacga tctgctggcg aagcggaaag atctcacctt cggcaatggc
     2641 gatcctaact ccacctctgg cttcctcgtc cccggttact acgtctttgc caaaaacaat
     2701 atctccgcca gcgactttaa gcgcaccgtc aacgctgggc atgaaaccaa cgcgctggcc
     2761 gtcgccaaca agcaggtgga tgttgccacc aacaacaccg aaaacctcga caagctgaaa
     2821 acctccgcgc cagagaagct gaaagaactg aaggtgatct ggaagtcgcc gctgatccca
     2881 ggcgatccga tcgtctggcg taaaaacctt tccgaaacca ccaaagacaa gatctacgac
     2941 ttctttatga actacggcaa aacgccggaa gaaaaagcgg tgctggaacg cctgggctgg
     3001 gcgccgttcc gcgcttccag cgacctgcaa ctggtgccga ttcgccagct cgcgctgttt
     3061 aaagagatgc agggcgtgaa aagcaataaa ggactgaatg agcaggacaa gctggcaaaa
     3121 accaccgaga ttcaggcgca gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg
     3181 atgagttcgg tgagtaaagc ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc
     3241 gcatccgaca atgtgcaggc gttgatgccg gatgcggtgc aagcacctta tccggcctac
     3301 agaccggagc caaacatgca aaccatcacc atcgccccac ccaagcgcag ctggttctcg
     3361 cttctgagct gggccgttgt tctcgccgtg ttggtcgtct cgtggcaggg cgcggaaatg
     3421 gccccgctca cgctgattaa agacggcggc aacatggcga cgttcgccgc cgacttcttc
     3481 ccgcccgatt tcagccagtg gcaggattac ctcaccgaaa tggccgtcac gctgcaaatc
     3541 gccgtctggg gcacggcgct ggcggtggtt ctctccatcc ccttcggcct gatgagcgcc
     3601 gaaaacctgg tgccgtggtg ggtttaccag cccgttcgcc gcctgatgga cgcctgccgc
     3661 gccattaacg aaatggtctt cgccatgctg ttcgtggtcg ccgtcggcct cggcccgttc
     3721 gctggcgtgc tggcgctgtt tatccacacc accggcgtgc tctccaaact gctttccgaa
     3781 gcggtggaag cgattgaacc cggcccggtg gaaggcattc gcgccaccgg cgccaacaag
     3841 ctcgaagaga tcctctacgg cgtgctgccg caggtgatgc cgctgctgat ctcctactcc
     3901 ctctatcgct tcgaatccaa cgtccgctcg gcgaccgtcg tcggcatggt cggtgcaggc
     3961 gggatcggcg tcaccctgtg ggaagcgatt cgcggtttcc agttccaaca aacctgcgcc
     4021 ctgatggtgc ttatcatcgt cacggtcagc ctgctggatt tcctctctca acggttgcgt
     4081 aagcacttta tctgataagc gaggcattga tatctatgca cttgtctaca catccgacca
     4141 gctacccaac acgctatcag gagatagccg caaaacttga gcaggagctt cgtcaacact
     4201 accgctgcgg cgactatctt cccgccgagc agcaactggc ggcgcgcttt gaggtgaacc
     4261 gccacaccct gcgccgcgcc atcgaccaac tggtggaaaa aggctgggta cagcgccgtc
     4321 agggcgtcgg cgtgctggtg ctgatgcgcc cgttcgatta cccgctcaac gcccaggcgc
     4381 gttttagcca gaacctgctg gaccagggta gccatcccac cagcgaaaaa ctgctgtcgg
     4441 tattgcgccc cgcgtccggt cacgtcgctg acgcgctggg gattaccgag ggcgagaacg
     4501 ttatccattt gcgcaccctg cgtcgcgtca acggcgtcgc gctctgttta atcgaccact
     4561 actttgcgga cctcaccctt tggccgacgc tgcaacgctt cgacagcggt tcgctgcacg
     4621 attttctgcg cgagcaaacc ggaattgcgc tgcgccgcag ccagacgcgg atcagcgccc
     4681 gtcgcgccca ggccaaagag tgccagcgtc ttgaaattcc gaatatgtcg ccgctgctgt
     4741 gcgtgcgcac ccttaaccac cgtgacggtg aaagcagccc ggcggagtac tccgtcagcc
     4801 tgacgcgcgc cgacatgatt gaattcacta tggagcactg aatgcacgca gataccgcga
     4861 cccgccagca ctggatgtcc gtactggcgc acagccaacc ggctgaactg gcagcccgcc
     4921 tgaaagcact gaacatcacc gccgactatg aggttatccg cgccgccgaa accggcctgg
     4981 tgcagattca ggcgcggatg ggcggcaccg gcgaacgctt ttttgccggc gacgccacgc
     5041 tcacccgcgc cgccgtacgc ctgactgacg gcacgctcgg ctacagctgg gtgctggggc
     5101 gcgacaaaca gcacgccgaa cgctgcgcgc tgattgacgc gctgatgcag caatctcgct
     5161 attttcaaaa cttatcagaa acccttattg ccccgctgga cgctgaccgt atggcacgca
     5221 ttgccgcacg ccaggccgaa gtgaacgcca gccgggtcga cttctttacg atggttcgcg
     5281 gagacaacgc atgaccctgg aaaccgcttt tatgcttccc gtgcaggatg cccagcacag
     5341 ttttcgtcgc ctgttaaagg ccatgagcga gccgggcgtg attgtcgccc tgcatcagct
     5401 caagcgcggc tggcagccgc tgaatatcgc caccaccagc gtgctgctga cgctggccga
     5461 taacgacacg ccggtgtggc ttgccgcgcc attgagtaac gatatcgtca gccagagcct
     5521 gcgttttcat accaacgcgc tgctggtcag ccagccggaa caggcgacct tcgcggtgac
     5581 ggatgaggcg atttccagcg aacagcttaa cgccctttcc accggcaccg ccgttgcgcc
     5641 ggaagcgggt gcaacgctga ttttacaggt cgccagcctg agcggcgggc gcatgttacg
     5701 tctcaccggc gcgggcattg ccgaagaacg aatgatcgct ccgcagctgc cggagtgcat
     5761 tctgcacgaa ctcaccgagc gcccgcaccc gttcccgctc ggcatcgacc tgatcctgac
     5821 ctgcggcgag cgcctgctgg ctattccgcg aaccactcat gtggaggtgt gctgatgtac
     5881 gttgccgtga aagggggcga gaaggcgatc gacgccgctc acgccctgca agagagccga
     5941 cgccggggcg ataccgattt gcctgaactg agcgtcgccc agattgaaca gcagcttaat
     6001 cttgcggtgg atcgcgtgat gaccgaaggc ggcattgccg accgcgaact ggcggcgctg
     6061 gcgctgaaac aggccagcgg cgataacgtc gaagcaattt tcctgctgcg cgcctaccgc
     6121 accacgctgg cgaagctcgc ggtaagcgag ccgctcgaca ccacagagat gcgtctcgaa
     6181 cggcgtattt cggcggttta taaagacatt cccggcggcc agctgcttgg cccaacctac
     6241 gactacaccc atcgcctgct cgatttcacc ctgctggcaa acggtgaagc gccgacgctg
     6301 accaccgccg acagcgaaca acagccgtcg ccgcacgttt tcagcctgct ggcgcgtcag
     6361 gggctggtga agtttgaaga agatagcggc gcacagccgg atgacatcac ccgcacgccg
     6421 ccggtttacc cctgctcacg ctcctcccgc ctgcaacaat taatgcgcgg ggacgaaggc
     6481 tatttgctgg cgctggccta ctccacccag cgtggttacg ggcgcaatca cccgttcgcg
     6541 ggcgagatcc gcagtggtta catcgacgtg tcgattgtgc cggaagagct gggatttgcg
     6601 gtaaacgtcg gcgaactact gatgaccgag tgtgaaatgg tcaacggttt tatcgacccg
     6661 ccggatgagc cgccgcactt cacgcgcggc tacgggctgg tattcggcat gagcgagcgc
     6721 aaagcgatgg caatggcgct ggtcgatcgt gcgttgcagg ctccggaata cggcgagcac
     6781 gcgacaggcc cggcgcagga tgaagagttt gtgctggcac atgccgacaa cgtcgaagcc
     6841 gcaggctttg tctcgcacct caaactcccc cactacgtcg atttccaggc cgaactggag
     6901 ctactcaaac gtctgcaaca ggagaagaac catggctaat ctgagcggct acaactttgc
     6961 ctacctcgac gagcagacca aacgcatgat ccgccgcgct atcttaaaag cggtggcgat
     7021 ccccggttat caggtgccgt ttggcgggcg cgagatgccg atgccatacg gctggggaac
     7081 cggcggcata cagctcaccg ccagcgtgat tggcgaaagc gacgtgctga aggtgattga
     7141 ccagggtgcg gatgacacca ccaacgccgt gtcgattcgc aacttcttta agcgcgtgac
     7201 cggggtaaac accactgaac gtacggacga tgcgacgctt atccagacgc gtcaccgcat
     7261 ccccgaaacg ccgctgaccg aagatcagat cattatcttc caggtgccaa tcccggaacc
     7321 gctgcgcttt atcgagccgc gcgaaacgga aacccgcacc atgcacgcgc tggaagagta
     7381 cggcgtgatg caggtgaaac tgtatgaaga tatcgcccgc ttcggtcata tcgccactac
     7441 ctacgcctat ccggtgaagg tgaacgggcg ctacgtaatg gacccgtcgc cgatcccgaa
     7501 attcgataac ccaaaaatgg acatgatgcc cgccctgcaa ctgttcggcg cggggcgcga
     7561 gaagcgcatc tatgcggtgc cgccgtttac ccgcgtggaa agtctcgatt tcgacgatca
     7621 cccgttcacc gttcagcagt gggatgagcc atgcgccatc tgcggatcga cccacagcta
     7681 tcttgatgaa gtggtgctgg atgacgccgg aaaccgcatg tttgtctgct ccgataccga
     7741 ttattgccgc caacagagcg aggcaaaaaa ccaatgaatc aaccgttact ttcggtcaat
     7801 aacctgaccc acctttacgc gccgggcaaa ggctttagcg atgtctcttt tgatttatgg
     7861 ccgggggaag tgctgggcat tgtcggggag tccggctccg ggaagaccac gctgctgaaa
     7921 tcaatctccg cgcgcctgac gccgcagcag ggggagattc actatgagag ccgttcgctg
     7981 tacgcaatga gcgaggccga ccgccgtcgc ctgctgcgca ccgaatgggg cgtagtgcat
     8041 cagcatccac tcgacggcct gcgccgtcag gtctcggcag gcggcaatat cggcgagcgg
     8101 ctgatggcga ccggggcacg tcattacggc gatattcgtg ccaccgcgca gaagtggctg
     8161 gaagaggtgg aaattcccgc caaccggatc gacgacctgc cgaccacctt ttccggcggc
     8221 atgcagcaac gtttgcagat cgcccgcaac ctggtgacgc atccgaagct ggtgtttatg
     8281 gacgagccga ccggcggact ggatgtgtcg gtacaggcac gcctgctcga cctgctgcgc
     8341 ggcctggtgg tggagctgaa cctcgcggta gtgattgtca cccacgattt aggcgtcgcc
     8401 cgcctgctgg cggaccgttt gctggtgatg aagcaggggc aagtggtgga gagtgggtta
     8461 accgaccgcg tgctcgacga cccgcaccat ccgtatacac aactgctggt gtcatcggtt
     8521 ttgcagaatt gagccggtgc cggatgcggc gtaaacgcct tatccggcct acaaatgcgc
     8581 tccccgtagg tcggataaga cgcgtcagcg tcgcatccga cacccgaacc acgaggcgaa
     8641 aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct gcaccagcaa aacggcgtgc
     8701 gcctgcccgt cttacaacgt gcctcgctca ccgtcaacgc gggcgaatgc gtggtgctcc
     8761 acggccattc cggcagcggc aaatcaacct tgctgcgctc gctgtacgcc aactatctgc
     8821 ccgacgaagg gcaaatccag atcaaacacg gtgacgaatg ggtcgatctg gtcaccgcgc
     8881 cagcgcgcaa agtggtggag atccgcaaaa ccaccgtcgg ctgggtgagc cagtttctgc
     8941 gcgtcatccc gcgtatttca gcactggaag tggtgatgca gccgctgctc gataccggcg
     9001 ttccgcgtga agcctgcgcc gctaaagccg cgcgtctgct cacccgcctg aacgtgccgg
     9061 aacgcctgtg gcacctggcg ccatcaacct tttccggtgg cgaacagcag cgcgtcaaca
     9121 tcgcccgcgg ctttatcgtc gactacccca ttctgctgct cgacgaacct accgcctcgc
     9181 tggacgccaa aaacagcgcc gccgtagtcg agctgattcg cgaagccaaa acccgtggcg
     9241 cggccatcgt cggcatcttc catgacgaag ccgtacgtaa tgacgtcgcc gaccgcttgc
     9301 acccaatggg agcctctgca tgattatcaa taacgtcaaa ctggtgctgg aaaacgaagt
     9361 ggtacacggt tcgctggagg tgcaggatgg cgaaatccgc gcctttgccg aaagccagag
     9421 ccgcctgcca gaagcgatgg acggcgaagg tggctggcta ctacccggcc tgattgagct
     9481 gcataccgac aacctcgata aattcttcac cccgcgcccg aaagtcgact ggcccgccca
     9541 ttcggcgatg agcagccacg acgcgctgat ggtggcaagc ggcatcacca ccgtactgga
     9601 cgccgtggcg attggcgacg tgcgcgacgg cggcgatcgg ctggagaatc tggagaagat
     9661 gatcaacgcc atcgaagaga cgcaaaaacg cggcgtcaac cgcgccgagc accgcctgca
     9721 cctgcgctgc gaactgccgc atcacaccac actgccgctg tttgaaaaac tggttcagcg
     9781 cgaaccggtg acgctggtgt cgctgatgga ccactcaccg ggccagcgcc agttcgctaa
     9841 ccgcgagaag tatcgcgaat attatcaggg caaatactcc ctcaccgatg cgcagatgca
     9901 gcagtacgaa gaagagcaat tggcgctcgc cgcacgctgg tcgcagccga atcgcgaatc
     9961 catcgccgcc ctgtgccacg cgcgacaaat tgcgctcgcc agccacgatg acgccacgca
    10021 cgcccacgtt gccgaatccc accaacttgg cagcgtgatc gccgaatttc ccaccacgtt
    10081 cgaagcggcg gaagcctcgc gtaagcatgg catgaacgtg ctgatgggtg cgccaaatat
    10141 cgtgcgtggc ggctcgcact ccggcaatgt ggcggccagt gaactggcgc agcttggtct
    10201 gctggatatc ctctcttccg actactaccc cgccagcctg ctggatgcgg cgttccgcgt
    10261 cgccgatgac gagagcaacc gctttacgct accgcaggcg gtgaggctgg tgaccaaaaa
    10321 tccggcgcag gcgctgaatc ttcaggatcg cggggtgatt ggcgagggta aacgcgctga
    10381 cctggtgctg gcgcatcgcc agggcaatca cattcatatc gaccacgtct ggcgtcaggg
    10441 taaaagggtg ttctgatgat gggaaaactg atttggttaa tggggccgtc cggctccggg
    10501 aaagacagcc tgctggcgga actccgcctg cgggaacaaa cgcagttact ggtggcgcat
    10561 cgctacatca cgcgcgacgc cagcgccgga agtgaaaacc atatcgccct gagtgagcgg
    10621 gagtttttta cccgcgcggg gcaaaacctg ctggccttaa gctggcacgc taacggtctg
    10681 tattatggcg tcggcatcga gattgacctc tggctgcacg ctggattcga cgtagtcgtc
    10741 aacggttcac gcgcgcattt gccgcaggca cgggcgcgct atcaatcggc gctgctgccc
    10801 gtctgtttac aggtttcgcc ggagatcctg cgccagcgcc tggaaaaccg tggccgtgaa
    10861 aacgccagtg aaattaacgc ccggctggcg cgcgccgccc gctatactcc acaggattgc
    10921 catacgctca acaatgacgg cagcctgcgc cagtcggtcg acacactgct gacgctgatt
    10981 catcagaaga agaaacacca tgcctgcttg tgagcttcgc ccggccacgc agtacgatac
    11041 cgacgcggtt tacgcgctga tttgtgagct aaaacaggcg gagtttgacc accacgcgtt
    11101 tcgcgtgggc tttaacgcca acctgcgcga cccgaacatg cgctaccatc tggcgctgct
    11161 ggatggcgaa gttgtcggca tgatcggcct gcatttgcag tttcatctgc atcatgtcaa
    11221 ctggatcggc gaaattcagg aactggtggt gatgccgcag gcgcgcggcc tgaacgtcgg
    11281 tagcaagttg ctggcgtggg cagaagaaga agcccgccag gccggggccg aaatgaccga
    11341 gctttcgacc aacgtgaagc gccacgacgc gcaccgtttc tatctgcgag aaggctacga
    11401 gcagagccac ttccgcttca ccaaggcgct gtaacatgag cctgaccctc acgctcaccg
    11461 gcactggcgg cgcacagggc gttccggcat ggggctgcga gtgcgcggcc tgcgccagag
    11521 cgcggcgctc gccgcagtat cgccgccagc catgcagcgg cgtagtgaag tttaacgacg
    11581 cgatcaccct gatcgacgcc gggcggcacg atctcaccga tcgctggtcg cctggatcgt
    11641 tccagcagtt tttgctgacg cactaccata tggatcacgt ccaggggcta tttccgctac
    11701 gctggggcgt gggcgatgtg atcccggtct acggcccgcc ggatgaacag ggctgcgacg
    11761 atctgtttaa acatccgggc ctgcttgatt tcagccacac agtggaaccg tttgtggtgt
    11821 ttgatttgca ggggttgcag gtcacgccgc tgccgcttaa ccactcaaaa ctgaccttcg
    11881 gttatctgct ggaaacggca catagccggg tggcgtggct gtctgacacc gctggcctgc
    11941 cggaaaaaac gctgaaattt ttactcaata atcatccgca ggtgatggtg attgattgca
    12001 gtcacccgcc gcgcgcggat gcaccgcgta atcactgtga tttaaatacc gtgcttgcgc
    12061 tgaatcaggt tatccgctcg ccacaggtga ttctgaccca tatcagccac cagtttgatg
    12121 cgtggctgat ggaaaacgca ctaccgtcag ggtttgaggt ggggtttgat gggatggaga
    12181 ttggggtggc gtgatgagag ggaatgtgcg cgctggcccc ctcaccctaa ccctctcccc
    12241 agaggggcga ggggaccgat tgtgctcaat attgaatatt gcgctcgttt tctccctctc
    12301 cccattgggg tgaggggcga tgcctgctcc atacccaacc tcatcgccca tactcatctt
    12361 ccattctccg ctcttcatcc tccagttgcc gacgctcctg atcaagctgg cgctggcgat
    12421 cgtccagctg cctgcggcga tcttcaaact ggcggcggcg gtcgtcatat tgtctgcgcc
    12481 gatcgtcgct cacttcacgc tgccagccgt cgtcgcgcga atcttcatag tctcgcccac
    12541 ggtcagggtt ataagcgtca ttaatcgcct gctgaatatt accaatagta tcgtcgataa
    12601 tatcagcctg ggccggaacg gtggacagcg tgagcagggt gaataaaaga aatagcggaa
    12661 agcgtttcat tagccaacct caaaaagaaa ctctatccac attagtcatt actcatccat
    12721 gcaagtagtg gatgaatctc aatttctccg ctgctctatt gccgtaatcg cctccacgcg
    12781 ttgttgatga cgaccgcctt cgtactgtgc gcccagccac gcatccacaa tcatttttgc
    12841 cagttcgagg ccaaccactc gtgaaccaaa agccagtgca acttcactgg cataatgcgg
    12901 ataatcagtg cgctctgacg acctggtgtc tttatcaatc acctcactca ttgaccatga
    12961 ataaaaggct ggcacagcaa cacagaatcc tgcggtgcca gcgacatgac cgcccattgc
    13021 tccggtacgt tttttaaaaa gaaacaccgg ttgcattgat aaattgtgct cagtatttgc
    13081 cgaagtacaa tatatataat aaatatataa aacacctcat attatttaca gagataataa
    13141 attcgcgtaa ttttccccct tcagcacaaa tgcctaataa aatggatcgt tccgctcaca
    13201 cttccactta ttaaagtcgc aaattcccaa ttatgataac gccagcttta aatgacgtat
    13261 tttgcatgga ttacatattt acgacacaaa tgataaatgg agatttatat gaataactct
    13321 cggttattcc gtttgagcag gattgttatt gcgttaactg ctgccagcgg catgatggta
    13381 aataccgcta acgcgaaaga ggaagcgaaa gccgccactc aatataccca acaggttaat
    13441 cagaattacg ccaaatcatt accgtttagc gatcgtcagg attttgacga cgctcagcgt
    13501 ggatttatcg ctccgctgct ggatgaaggt attctgcgcg atgctaatgg caaaccatat
    13561 tatcgcgggg aagattataa atttgatatc aatgcgcccg cgcccgaaac cgttaaccct
    13621 agcctgtggc gtcagtcgca gttaaacggt atttccggcc tgtttaaagt caccgacaga
    13681 atgtaccagg ttcgcggtca ggatatctcc aacatcacct ttatcgaagg tgataccggg
    13741 attattgtca tcgacccgct ggtgacgcca ccgagcgcaa aagccgccct tgacctttac
    13801 ttccaaaatc gcccgcaaaa accgattgtt gcggttattt atacccacag ccataccgac
    13861 cactatggcg gcgtgaaagg cattatctct gaagctgatg tgaaatccgg taaagtgcag
    13921 gttatcgccc ctgctggctt tatggacgaa gccatcagcg aaaacgtact ggcgggtaat
    13981 attatgagcc gccgtgcact ttactcctac ggcctgctgc tggcgcataa ccctcagggt
    14041 aacatcggca atggtcttgg cgtaacgctg gcatcgggct acccgagcat catcgcaccg
    14101 aacaaaacca tcaccaaaac cggtgagaag atgattatcg acggcctgga gtttgacttc
    14161 ctgatgaccc caggtagcga agcaccagcc gaaatgcact tctatattcc ggccctgaaa
    14221 gcgctgtgta ccgccgagaa cgccacgcat accctgcaca acttctacac tctacgcggc
    14281 gcgaaaaccc gcgacaccag caagtggacc gagtatctga acgaaacgct ggatatgtgg
    14341 ggtaacgacg cggaagtcct gtttatgccg cacacctggc cggtctgggg caataagcat
    14401 atcaatgatt atattggtaa atatcgcgat actatcaagt acattcacga ccagaccctg
    14461 cacctggcga accagggcta caccatgaat gaaatcggcg acatgattaa actgccgcct
    14521 gcacttgcca ataactgggc cagccgtggc tattacggtt ctgtcagcca caacgcccgc
    14581 gcggtgtata acttctatct tggctattac gacggtaacc cggctaacct gcatccgtat
    14641 ggtcaggtgg agatgggtaa acgttacgtg caggcgctgg gcggttctgc ccgtgtcatc
    14701 aacctggcgc aagaagcgaa caagcaaggt gattaccgct ggtcggcaga actgctgaaa
    14761 caggtgattg ccgccaaccc gggtgaccag gtcgcgaaga atctgcaagc gaataacttt
    14821 gaacagctgg gctatcaggc cgagtccgcc acctggcgcg gtttctacct gaccggcgcg
    14881 aaagagctgc gcgaaggggt gcataagttc agccacggca ccaccggttc cccggacacc
    14941 attcgcggga tgtcggtcga aatgctgttc gactttatgt ccgttcgcct cgatagcgcg
    15001 aaagccgcgg gtaaaaatat cagcctgaac ttcaatatga gcaatggcga taacctcaac
    15061 ctgacgctga acgatagcgt gcttaactac cgtaaaacac tgcaatccca agctgacgcc
    15121 tctttctaca tcagccgtga agatctgcac gccgtgctga ccgggcaagc caaaatggcg
    15181 gatctggtaa aagcgaagaa agccaaaatt attggcaatg gcgcgaaact ggaagaaatt
    15241 atcgcctgtc tggataattt cgatttgtgg gtgaatatcg taaccccaaa ttaatgtcac
    15301 tcgcttttgg gggcgctgaa gcccccaaaa gcatttattt taaaaatata tacagccaat
    15361 taataaataa aatatataag ttatgaatgc ggtggctgaa taaagctatt tttattcatc
    15421 gagaaaatag ccatactgac gccttcaatg gatattgata ttcactacag atattgttcg
    15481 cagcagacag gatgtattta atatgccaac agttctctct cgcatggcga tgcaactcaa
    15541 aaaaacagcc tggataattc ccgtcttcat ggtttcggga tgctcattat ctccggcaat
    15601 cccggtgatc ggtgcttatt atcccagctg gtttttctgc gccattgcca gccttatttt
    15661 gatgctcatc acgaggcgag ttattcagcg ggcaaatatc aatctggcat ttgtcggaat
    15721 tatttatacc gccctttttg ctctctacgc catgctgttc tggctggcat ttttctaatc
    15781 tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta aattccctgc
    15841 tctgttagtg gttgcgttgg cgctggttgc ccttgttttc gttatctggc gcgtagacag
    15901 tgcgccatca actaatgacg cttacgcgtc agcagatacc attgatgtga tgccggaagt
    15961 cagcggtcgc attgtagaac tggcggtcac cgacaaccag gcagtcaaac agggcgattt
    16021 gctgttccgc attgacccgc gcccgtacga agccaatctg gcgaaagctg aagcctccct
    16081 cgcggcgctg gataagcaaa ttatgctcac ccagcgtagc gttgacgcgc aacagtttgg
    16141 tgccgactcg gttaacgcca cggtagaaaa agcccgtgcc gccgcgaaac aggccacaga
    16201 tacattacgc cgcaccgagc cattactgaa agaaggtttt gtctcagcgg aagacgttga
    16261 ccgtgcgagg accgcgcagc gcgctgcaga agccgatctt aatgctgtat tgttgcaggc
    16321 gcagtcagcc gccagcgccg tcagcggcgt ggatgcatta gttgcccagc gtgcggcggt
    16381 cgaagcggat attgccctga ccaaactgca tctggaaatg gcgaccgttc gcgcgccgtt
    16441 tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg ccatgcgccc
    16501 tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc gccaacttcc gcgaaaccga
    16561 tctgaaaaat attcgctcag gtacacccgc aacgattcgc ctgatgagtg acagcggcaa
    16621 aaccttcgag ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg acggcggcct
    16681 ggtgctgggc ggcctgccga aagtttctcg ctctattaac tgggtccgcg ttgcccagcg
    16741 ttttccggtc aaaatcatgg tcgataaacc tgacccggaa atgttccgca tcggcgcttc
    16801 ggcagtcgct aatcttgagc cgcaataatg agcgcgctca actccctgcc attaccggtg
    16861 gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc ggcgaccagg ccgcgtgccg
    16921 cagaccgtgc aactctgggt aggctgcctg ctggtgattc tgatctcgat gacctttgag
    16981 atccctttcg tggcgttatc gctggcagtg ctgttttacg gtattcagtc gaacgcgttt
    17041 tacaccaaat ttgtcgcgat cttatttgtg gtcgccacga tgctggagat cggcagcctg
    17101 tttttgatct acaaatggtc atacggcgaa ccgttgatcc gattgttcat cgccgggccg
    17161 atcctgatgg gctgtatgtt tttgatgcgc acccatcggc tgggactggt ctttttcgca
    17221 gtcgccattg tcgccattta cgggcaaacc ttccccgcca tgctcgacta tccggaagtg
    17281 gtcgtgcgct taacgctgtg gtgtatcgtt gttggccttt atccaacctt gctgatgacg
    17341 ttaatcggcg tgctgtggtt tcccagtcgt gccatttcgc aaatgcatca ggcgcttaat
    17401 gatcggcttg atgatgccat tagccacctg acggacagcc tcgcaccgct acccgaaacg
    17461 cggattgaaa gagaggcgct ggcgctgcaa aaactgaatg tcttttgtct cgcggacgat
    17521 gccaactggc gaactcaaag cgcatggtgg caaagctgcg tggcaacggt aacctacatt
    17581 tactcgacgc tgaatcgcta cgatcccacc tcttttgctg attctcaggc aattattgaa
    17641 tttcggcaaa aattagcctc agaaatcaat aagctgcagc atgccgttgc tgaaggtcag
    17701 tgctggcaaa gcgacaggcg gatcagtgaa agtgaagcga tggcggcacg ggaatgtaac
    17761 ctggagaata tctgccagac gttgttacaa ctgggtcaga tggacccgaa tacgccgcca
    17821 acgcccgcag ccaaaccgcc atcaatggcc gccgatgctt ttaccaatcc agactatatg
    17881 cgctacgcgg taaaaacgct gctcgcctgt ttgatctgtt acacctttta cagcggcgtg
    17941 gactgggaag gcattcacac ctgtatgctg acatgcgtga tcgtcgctaa cccaaatgtc
    18001 ggttcgtcgt accagaagat ggtgctgcgt tttggcgggg ccttttgcgg cgcgattctg
    18061 gcgctgttat tcacgctact ggtcatgccc tggctggaca atattgtcga attgctgttt
    18121 gtgctggcac cgattttcct gttgggcgca tggattgcca ccagctctga acgctcttct
    18181 tatatcggca cacagatggt ggtcaccttc gcgctcgcca cgctcgaaaa cgtttttggc
    18241 ccagtgtacg acctggtgga aattcgcgat cgcgccctgg gtatcatcat tggtaccgtg
    18301 gtgtccgcgg tgatttacac ctttgtctgg cctgaaagtg aagcgcgcac actgccgcaa
    18361 aaactggctg gcacgctggg tatgttaagt aaagtaatgc ggatcccacg ccagcaggaa
    18421 gtcacggctc tgcgcactta tctgcaaatt cgtatcggtc tgcatgcggc gtttaatgcc
    18481 tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac tggacagcga agaacgcgcc
    18541 ttactgattg aacgttcgca aacggttatt cgtcagggcc gcgatattct tcacgcctgg
    18601 gatgccacct ggaactcggc gcaggcgctg gataacgcac tacagccgga cagagcagct
    18661 cagtttgccg acgccctgga gaaatacgct gccggtctgg caaccgcact cagccgttct
    18721 cctcaaataa cgcttgaaga gacacccgcc tcgcaggcca tcctgcccac cttattaaaa
    18781 caggagcaac acgtctgcca gcttttcgcc cgcttgccag actggacagc cccggcatta
    18841 acgcccgcca cggaacaggc acaaggagcc acgcaatgat caatcgtcaa ctttcacgtc
    18901 tgctgttgtg cagcattctc ggcagcacga cgctgatttc cggctgtgcc ctggtacgta
    18961 aggattctgc acctcatcaa cagctcaaac cggaacaaat caaactggcc gacgatattc
    19021 atcttgccag ctccggctgg ccgcaggcgc agtggtggaa acaactcaat gacccgcagc
    19081 tggatgcgct gatccaacgg acgctaagtg gttcacacac cctcgccgaa gcgaaactgc
    19141 gggaagaaaa agcgcagtcc caggccgatt tgttagatgc cggttcacaa ttacaggtcg
    19201 cagcgttagg gatgctcaac cgccaacgcg tctcggcgaa cggcttttta agcccttatg
    19261 cgatggatgc gcccgcactg ggtatggacg ggccgtacta tacggaagcc acagtaggtt
    19321 tgtttgccgg actggatctt gatttgtggg gtgtgcatcg ctcagcggtt gccgccgcca
    19381 ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt agagctatcg ctgaccacgg
    19441 gcgtagcgca gctttattac agtatgcagg ccagctatca gatgctcgat ctgttagaac
    19501 aaactcgcga tgtgattgat tacgcggtga aagcgcacca gagtaaagtg gcgcacggtc
    19561 tggaagcaca agtgcctttc cacggcgcgc gggcacagat tctggcggtc gataaacaaa
    19621 ttgctgccgt caaagggcaa atcaccgaaa cgcgagaatc tctgcgtgca ttgattggcg
    19681 cgggggccag cgatatgccg gagatcaaac cggtggcatt accacgagtc cagaccagca
    19741 ttccggcgac actctcttat gagttgctcg ccagacgccc ggatctgcaa gccatgcgct
    19801 ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg ggcgctgttc tacccgagct
    19861 ttgatatcaa agcgtttttc ggccttgatg ccatccacct ggatacctta ttcaaaaaaa
    19921 ccagtcgcca gttcaacttc atcccgggtc tgaaattgcc gctgtttgac ggtggacggt
    19981 tgaatgccaa tctcgaaggc acgcgcgccg ccagcaacat gatgattgaa cgttacaacc
    20041 agtcagtact gaacgcggtg cgtgacgttg ccgtcaacgg cacgcgtctg caaacgctca
    20101 acgacgagcg agaaatgcag gctgaacgcg tggaagcaac gcgcttcacc cagcgcgctg
    20161 ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca ggccactgaa gcccggttgc
    20221 cagtacttgc cgaagagatg tcattactga tgctggacag ccgccgggtg atccaaagca
    20281 ttcagttgat gaaatcgctg ggcggcgggt atcaggcggc tcccgtcgtc gagaaaaaat
    20341 aaaatgtctg ccgcgtgatg gctgtcacgc ggtatttcgt ttcgtcacgt caaaactgac
    20401 gacagcctgt ttttcgtcag agttttgaat aaatagtgcc cgtaatatca gggaatgacc
    20461 ccaaataaaa tgtggcataa aagatgcata ctgtagtcga gagcgcgtat gcgtgatttg
    20521 attaactgga gcgagaccga tgaaaaaagt cgtcacggtt tgcccctatt gcgcatcagg
    20581 ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc cgggcggagg cagcgcaggg
    20641 gaaaaccaac cagggtaccc tgtgtctgaa gggttattat ggctgggatt tcattaacga
    20701 tacccagatc ctgaccccgc gcctgaaaac ccccatgatc cgtcgccagc gtggcggcaa
    20761 actcgaacct gtttcctggg atgaggcact gaattacgtt gccgagcgcc tgagcgccat
    20821 caaagagaag tacggtccgg atgccatcca gacgaccggc tcctcgcgtg gtacgggtaa
    20881 cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt attggtacca ataacgttga
    20941 ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt ctgcaccaat cggtcggtaa
    21001 tggcgcaatg agcaatgcta ttaacgaaat tgataatacc gatttagtgt tcgttttcgg
    21061 gtacaacccg gcggattccc acccaatcgt ggcgaatcac gtaattaacg ctaaacgtaa
    21121 cggggcgaaa attatcgtct gcgatccgcg caaaattgaa accgcgcgca ttgctgacat
    21181 gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg aatgcgatgg gccatgtcat
    21241 tattgaagaa aatctgtacg acaaagcgtt cgtcgcttcc cgtacagaag gctttgaaga
    21301 gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt gaagatatca ccggcgtcag
    21361 cgccagtgag attcgtcagg cggcacggat gtatgcccag gcgaaaagcg ccgccatcct
    21421 gtggggcatg ggtgtaaccc agttctacca gggcgtggaa accgtgcgtt ctctgaccag
    21481 cctcgcgatg ctgaccggta acctcggtaa gccgcatgcg ggtgtaaacc cggttcgtgg
    21541 tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg ccggatacgt atccgggata
    21601 ccagtacgtg aaagatccgg ctaaccgcga gaaattcgcc aaagcctggg gcgtggaaag
    21661 cctgcctgct cataccggtt atcgcatcag cgagctgccg caccgcgcag cgcatggcga
    21721 agtgcgtgcc gcgtacatta tgggcgaaga tccgctgcaa actgacgcgg agctgtcggc
    21781 agtacgtaaa gcctttgaag atctggaact ggttatcgtt caggacatct ttatgaccaa
    21841 aaccgcgtcg gcggcggatg tcattttgcc gtcaacgtcg tggggcgagc atgaaggcgt
    21901 gtttactgcg gctgaccgtg gcctccagcg tttcttcaaa gcagttgaac cgaaatggga
    21961 tctgaaaacg gactggcaaa tcatcagtga aatcgccacc cgtatgggtt atccgatgca
    22021 ctacaacaac acccaggaga tctgggatga gttgcgtcat ctgtgcccgg atttctacgg
    22081 tgcgacttac gagaaaatgg gcgaactggg cttcattcag tggccttgcc gcgatacttc
    22141 agatgccgat caggggactt cttatctgtt taaagagaag tttgataccc cgaacggtct
    22201 ggcacagttc ttcacctgcg actgggtagc gccaatcgac aaactcaccg acgagtaccc
    22261 gatggtactg tcaacggtgc gtgaagttgg tcactactct tgccgttcga tgaccggtaa
    22321 ctgtgcggca ctggcggcgc tggctgatga acctggctac gcacaaatca ataccgaaga
    22381 cgccaaacgt ctgggtattg aagatgaggc attggtttgg gtgcactcgc gtaaaggcaa
    22441 aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa ggggcgattt acatgaccta
    22501 ccagtggtgg attggtgcct gtaacgagct ggttaccgaa aacttaagcc cgattacgaa
    22561 aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg atcgccgatc agcgcgccgc
    22621 cgagcagtac gtgattgacg agtacaacaa gttgaaaact cgcctgcgcg aagcggcact
    22681 ggcgtaatac agtcctttct acagcctcct ttcggaggct gtttttttat ccattcgaac
    22741 tctttatact ggttactccc tacccaatcg tattatcaaa atgaaaaaaa ttatcgcatt
    22801 gatgttgttt ttgacattct ttgcccacgc gaacgactcc gagcctggca gccagtattt
    22861 aaaggcagca gaggccgggg accgacgcgc acaatatttt cttgccgaca gctggtttag
    22921 ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa gccgccgaca gcggtgatgc
    22981 tgatgcctgc gcgctgctgg cgcagatcaa aatcaccaat ccggtcagtc tggactatcc
    23041 acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc agtaaagaag gtgaagtaac
    23101 gctggcgcat attctggtaa atactcaagc gggtaaaccg gattatccaa aggcgatttc
    23161 gctgttagaa aacgcctcgg aagatctgga gaatgactcc gccgtcgatg cccaaatgct
    23221 gcttggtttg atttacgcca acggcgtggg cattaaggcc gacgatgaca aggcaacctg
    23281 gtatttcaaa cgcagctctg caatttcccg aaccggttat tccgagtact gggcgggaat
    23341 gatgttctta aacggtgagg aaggttttat cgagaagaac aaacaaaagg cgctgcactg
    23401 gttgaacctg agctgtatgg aagggtttga taccgggtgt gaagagtttg aaaaattaac
    23461 caacggttaa ggtaggcctg ataagacgca ccagcgtcgc atcaggcatc cacacattgc
    23521 cgggtggata accccggcaa tcttcaatta ttgatccgca gttttatcaa attagcccag
    23581 cacttcacgc tcataagcca gcgccttttt gcggtcaaat ttgtgttccc acttggcaat
    23641 caccagcacc gccagcgcat tacccaccac gttcagcgca gtacgcgcca tgtcgaggat
    23701 acggtcaaca ccagcaataa acgccaggcc ttccagcgga atacctacgc tacccagcgt
    23761 tgccagcagc accacaaacg acacgcccgg cacgccagca atccctttcg aggtcaccat
    23821 cagcgtcagc accagaatga tttcctgcca gatggacagg tcaatgccat acagctgagc
    23881 gatgaaaata gcggcaatac tttgatacag cgtcgaacca tcaaggttaa aagagtaacc
    23941 ggtcggcacc acgaagctgg tgatcgacgc cggtgctccg taggcttcca tcttctcaat
    24001 aattcgcggc agtacgcttt cagagctggc agtggagtac gccagaatca gctcatcttt
    24061 cagaatacga atcaggatcc agacgcttaa cccgcacagg cgcgccacaa ttcccagcac
    24121 taccagcgcg aagaacagaa tggcgaaatg caccagcagc accagtttcg ccagtggcca
    24181 cagagaagag aaaccaaagt tagccaccgt caccgcaatc agcgcaaaca cacccaccgg
    24241 tgcataacgc atcaccatgt gagtcacttt aaacatggtt tcagagatgg agcggaacac
    24301 ggtcaccagc ggttcacgat gcgtcgcggg cagggaggaa agccccagac caaacagcac
    24361 cgagaagaag atgatcggca gcatttcgcc tttcgccatc gacgccacaa tgttcgtcgg
    24421 caccagcgac aaaatcgtgc ccataatgcc gtgggaactg ctttgtaccg cttccgtagt
    24481 gctctgatat ttcgagatat cgacggtcgc cagctgcgac atatccaccc cggcaccggg
    24541 ctggaagacg ttcgccagag tgatccccaa aatgatggcg acggtggtga tcacctcgaa
    24601 gtaaataatg gttttcgcgc caatacgccc gagctgtttg gcatcaccaa cacccgcgat
    24661 acccaccacc agcgtggaga tcacaatcgg cacaacaatc attttgatca gatggatgaa
    24721 gatatcaccc gccggagaga gcaaattgac gaccagccag tcgcggctgt cgctatggta
    24781 gtgcaggtag cttcccagga gaatgcccag caccatagca aacagaatct gccaggccag
    24841 gctgaatttt atacttttca tcagaataac ttcctcaatg gagcgccgtt ccccgttcag
    24901 aaacgcgaga acgtgacata ctgccagggc atgaattgtg ttgcgttggt ttgtagattt
    24961 ttttaacgct tatgatcagt atcatttgcg cggtatgttg cgcaaccctt taacaacaga
    25021 gcttttcgct gcattaatgc ataataacgc gagtttatgg caggattaat gaataaccat
    25081 ttgttatgga aagattttta tttgtttaat ctgcaataat actgattggt gattattttt
    25141 ggcatatgtt cattcgatta ttttttatac aactcaataa cataatcttc atcaccagat
    25201 gattaatctg atctccgctg caacaatttc gccatattaa tcgactcgac cagctgtgtc
    25261 cggttgatcc ggggtgagtt gagatccatc actttttgcc atagttcgat ggcttgcgcg
    25321 taattcgcct gcataaacgc atcggaagcc agcagcatca gagcggtgat ttcgttagag
    25381 tccagcgcga gggctttgtc gatcattgcg cgagtctggg cggtcatatg ctggctggcc
    25441 tggtaataaa gcaccgtcgc cagcgccgca tacagttcag cgttctcacc acgtagttgc
    25501 aacgcctgac ggtacgccag cagcgaattg ctgtaatcgt tttgccacag atagtactcg
    25561 cccagtaacg cccattgttc gctgttttgt ggattagcgc ggattttatc ctgcaatgcc
    25621 tgaagctgcg cttccggggt ttgctggctg gcaaactgat gtagcggatc gcgctgacgc
    25681 tgatattccg catgtaccgc ctgccatttt ggacttaaca gataactgcc gacacagaga
    25741 aataccatca aaatcgtgag tgttgtcagc attttcaccg gtattttggg ctctttcatc
    25801 gcttcgccct cactcgccag aggatcagtg ccatcagcag taacaacacc actggcagcg
    25861 cccacaacac cagcgtctga cccgttaacg gtgggttata gcgaacaaaa tctccgtagc
    25921 gttcggtcat ccagccaatg atttcgactt cgttcttccc ctccgccacc atgctgtaaa
    25981 cctgatggcg catactgact gccaccggcg cgttggattc cagtaagttt tgattctggc
    26041 actgcggaca acgtaactgg ctggcaatat ttaacgcctg ttgctgttgt tgcggattgg
    26101 cgaattgcca ggtgtctacg acctgagcgc gcgcaaaaca ggtaaataac agcagtagcg
    26161 tgagaagtcc tttattcatc gcgcttcctc ccccgccatc cgcttaacaa tgcgcccgca
    26221 atcatcaaca atcctccccc ccagatccag cgcacaccgc tttgtacata caagcgaaaa
    26281 gcgtaacgat ccgctccggt tttttcaccc atgaccgcat accagtcatg gatgccgttc
    26341 cagcgaattg acggttccat catttgctga cgacgtgctt cgtaaaaacg ccgctccggc
    26401 atcagttcac caatgcgttg ctgatggtca aacagtgcca ctatcgcttt ttcgctggtg
    26461 taattgcctt tggcttgcag atcgaggcgc tcaaaacgga aggtgtatcc tgccagcgtc
    26521 acctgctgac ccggctgtaa attcaggctg atctcctgac ggctgacgct ggaaaccacg
    26581 atccccgcgg caaataacag cacgcccgca tgagctacca gcgccggaag ctgcacgcgt
    26641 ttgccagaga caaacgtcgc cagcacaatc accaccagca tcaacaggcc aaacggtaac
    26701 gtcgcgcggt taaaatacgg cgcgccaacg gagaggcgtc cccagcccag caggccgtaa
    26761 atcatcggat aaagcgttcc caccagcacg atcagtagca ctgcgcaaaa cagcaacagc
    26821 gtagcgagga ttaacatttc ccgcgataac cccgaaaaac gcaccaccgc accaccgtcc
    26881 cgcgctcgcc agccatacag agccagagac gcaaggctaa tcagtgcaaa caggctgaac
    26941 aacggcacgg cgcggacgtt atccagtgcg aacgcatgaa ccgaaaccag aatgccagaa
    27001 cggacaatta aggtacccag tagcgacagc atcagagtta ctatcgccag caacagcgac
    27061 cagtggcgga aaatctcccg ctggcgtgtc agggacaaac tgtgcagcag cgcagtggca
    27121 gaaagccagg gtaataaaga ggcgttttcc accggatccc agaaccacca gccgccccag
    27181 cccagttcgc aataggccca ccaggaaccg aggatgatcc ccgccgttaa tgcgctccag
    27241 ccaggtagtg cccagcgcca acaaattctg gcgcaggcac catcaaactc gccgcgcagt
    27301 aaactcgcca gcgccacgct cgccgctacc atcaaaccgc catagccaag gtaaagcagc
    27361 ggtggatgaa agataagacc gggatgttgc agcatcggat tgagatcgcg gccttcgatt
    27421 gctggtggaa atatccgcac gaagggatcg gaccacagca ccacaaacag tagcagtgcg
    27481 gcgagcatga gagataaaac ggctaacgtc agcggaaata gcggatcggt ttgctgccga
    27541 taatgccagg caaacagcgc gctccaggcg gaaagcagca gcacccaaag cagcagcgaa
    27601 ccttcatggc cgccccacac cgctgccagc ttgagttccc acgacagcag gctatagcta
    27661 tgttgcgcga cataaatgac cgagaaatcg ctgatgagaa aacaatacgt cagtacgcca
    27721 aaggcgagca gcaggagcgc gaactgcgcc agaatgccga tgcaagtgag tcgcatcatg
    27781 gcaggccagc gcaaccgcac tcccgcaaag gccgtcaacg gggtcaacac gttgaccccg
    27841 agacttaaca acaacgccag aaaaccgact tcaggaagga atacgtccag aaaccacctc
    27901 ttttctggct tacgccaccg tcaactgtcc ggcgtagaga atgaaaaagc gcagcatcaa
    27961 cacgccgacc agactggccc cacacgccgc cagcacggca ggaatgccgg aactgcgatt
    28021 gacccacggt ttgagcaaca ttggcacaat cagccccagc ccggcgacac caagccagaa
    28081 ccaccacgtc cagaaaccgc cacctaatgc cgccaccagc gcacgcactt taccgtcatc
    28141 gcccagcgcc agaccgacaa aaaatgccac cagcaggaag atttcacccc ataccacggg
    28201 gatttccata cggtgtacaa actgcgcttc cgtggaatgc gggttactgc gttggcgtat
    28261 cgccatggcg atcagcgcca ccgccgcacc ggacgagatg ccggagaaga ggaacagcac
    28321 cggcaggatc gggttattga ggaacggata cgatttcagc gccgacagca gaaagccggt
    28381 ataagccccc aacaacaccg ccaacaccag catcaatgtt tccagtccgc gatgaacggg
    28441 cgtcagcagg ctcagaacct tttgcacgat ccccagcttc ggcaaccagc gttgttgcag
    28501 ggcaagcaaa tcatgttcaa agattttcgc cagccacagc accagcacca ccatgtagag
    28561 ctgaaacagc atcaccccca tcgacatcac cgaggtaaaa ctgtagtgga acatcagctt
    28621 ccagaaggtc cacggtcttg tcaggtggaa gacgaggatc aacagaccga ggatcaccgc
    28681 gcccggcccg acaatcagcg tggtgcgcag caacgtactg tctgcaccgc ccgcctgcgg
    28741 gtagaagcga cgtaacagca cggccagcgt caccagaccg gcagaaatac caatcaaaaa
    28801 caggtagatg gcaatcggcc agtcccacac cagcgattca aaatgaaatg cggaagtctg
    28861 cgtcattggc tcacctcccc gtatttaaac ggtacgcggt acagcttcgg tttggttccc
    28921 agcgccagct tgtagcggta agtaggcttc tggcgcagca gttgcgaaat ctcactgtta
    28981 ggatcgtcca gattgccaaa cgtcagcgcc ttcgtcgggc aagcctcaac gcacgcgggc
    29041 agcttaccgg cctgcaaatt ggttttacgg cagaaatcgc atttgtccgc cgtcttcgtg
    29101 accggatgga taaagcgcac gcggtacgga caggcggcga tgcagtactg acaaccgacg
    29161 cagagatcag gattaacatc gacaatcccg ctagcggcat cacgaaaaga cgcaccggtc
    29221 gggcagacgt caacgcacgg cgcatgatcg cagtgctggc aagacttacg gaagaagcga
    29281 tatttcacat caggaaattc gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg
    29341 ccttccggca ccttgttcac ttcccgacaa gcatccatac aggcggtaca gccgatgcat
    29401 aacgactcgt catgcaccat gccgtaacgc accccattga tattcaacgt tttcgccacc
    29461 acgcgccctg cggtgccgct gacggctgcc agcacgccga cgccggtgag aaactggcga
    29521 cgagaccagg tcatggctgc tccttaagca acggaacaga cgccgggtta aagttcggat
    29581 tggtgcgctg atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg tcacttaacg
    29641 tctgcatcgt atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc actttggtta
    29701 cgtggacatc gtgcggccag aacgcttttt gcaactgttc cggcagatga caggacatac
    29761 agacgctgtt ctgctctcca accttgtaca tcggctcgtt aaagcgcatc acatctttca
    29821 ccccttcgcg gtgttgcggt gatggctggc cgtgacagtt ggtgcaggtg accggcagtt
    29881 tgttattcgg gttgataacg gaagcatgtt ttccatgcat accttcggta tctggtttat
    29941 gacagtccag acaggcggca tccgggttac gctgctgggt aacttcgtag cgatcatcag
    30001 atgcctgcgc cgcaggggta gcggtaattc cgttcaggct ccacaacaag cccgacgcca
    30061 gcaccccggc agttaataac gaacgtaata cgctcatatt cactccattg tgttttccgc
    30121 cccttgcgag gcggaacggg gttattggct taacagacca tttttacgtg cctgttcttc
    30181 ccactgcggg atcaccgttt tgatgaagtc ctgcttctcg gccttgattt gttccatgtt
    30241 caggccaatg gcctgctggg ctttctcttt ggtcgagata tccgggatct ggatttcatg
    30301 ggtgatgcct ttggtcgcca gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc
    30361 catcgccgta ccgagcatcc gtagaccttc ttccggtgcg tgcatatgaa taccgtggga
    30421 agcgatcgcc agatcccaac gccactgggc atgacggata tcgtcctgaa tcggcttcat
    30481 ttcggcttcc gtcgcgcctg catccagcgc tgctttcgct tcgaagtgag cgtgaaccag
    30541 ttgatcttca accttgattt tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt
    30601 ttgcaaagca gctttgtcct gggtatggca gttcgcacaa gtctgggcga agttatcaaa
    30661 cggattacca attttatggt cggtgtagag tttgccttcg gcgttctgca cttttggcat
    30721 atggcagtcg atacaggtca cgttgttttt accgtgaatg cccgctgtcc aggtttcata
    30781 ttcagggtgc tgcgctttca gcattggcgt tttcgacagg gagttagtcc agtcagagaa
    30841 ggcaattttg tcgtaatact gctccatatt ttcgactttc atgccgtcat cccacgggaa
    30901 tttaaccgct ttgtttttgc cgtcgaagta atactccaca tggcactgac cgcaaaccat
    30961 cgattgctgg tcgaaacgtc cggctttctc aaaaggttta ccaatggctt ccatcgcgcg
    31021 agccgcatac ggacgggaaa gggttaactc cggtttacct ttggcgaact ctggtgaggc
    31081 ggtgttatgg cagtcggcac aacctaagtt gttgacgatt tccgggccgc cgcgcgccca
    31141 cttaccgtgg aagtagccat cttcgccgtc tttctggatc agacgcgcca catccgggct
    31201 tttacaactc cagcacgcca tcggtagcgg accatcttca gcgtttttcg gcgcaccggt
    31261 acgcagggtt tcacgcacat cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc
    31321 gcgcgagaag ggataccccg cccacaggat caccagccgt ggatcttccg ccagggcgtc
    31381 aacacgctct gactgttccg aggtggcttt ccaggagaga tattgatcgg gatgctgcgg
    31441 ggcaaaggtt tcattcttcg cttccacagt tacaggtttt gcgggagctg ccgtttgttc
    31501 agcgtgaaca gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt
    31561 tatttttatc cttgtcatag gggcttcatc cgaattgcgc cattgttgca atggcggttt
    31621 ttattgtttt cacgacagta accgcaccta cactgtcatg acattgctcg cccctatgta
    31681 taacatataa ccatactgtg aatgttgtct ttaatcaatt gtaagtgcat gtaaaatacc
    31741 actttagagt tagtcagtat cttcctcttt ttcaacagca tgcataactg catgttcctc
    31801 aaagaattaa tcaacttttg ttggtgacct tcaaaaatta ccctgccgtt tatttgcaca
    31861 attctacttt tgcgtgatct gtcgcccaaa tactaaacaa aactgccaat acccctacat
    31921 ttaacgctta tgccacatat tattaacatc ctacaaggag aacaaaagca tgagccaaat
    31981 tcacaaacac accattcctg ccaacatcgc agaccgttgc ctgataaacc ctcagcagta
    32041 cgaggcgatg tatcaacaat ctattaacgt acctgatacc ttctggggcg aacagggaaa
    32101 aattctcgac tggatcaaac cgtaccagaa ggtgaaaaat acctcctttg cccccggtaa
    32161 tgtgtccatt aaatggtacg aggacggcac gctgaatctg gcggcaaact gccttgaccg
    32221 ccatctgcaa gaaaacggcg atcgcaccgc catcatctgg gaaggcgacg acgccagcca
    32281 gagcaaacat atcagctata aagagctgca ccgcgacgtg tgccgcttcg ccaataccct
    32341 gctcgagctg ggcattaaaa aaggtgatgt ggtggcgatt tatatgccga tggtgccgga
    32401 agccgcggtt gcgatgctgg cctgcgcccg cattggcgcg gtgcattcgg tgattttcgg
    32461 cggcttctcg ccggaagccg ttgccgggcg cattattgat tccaactcac gactggtgat
    32521 cacttccgac gaaggtgtgc gtgccgggcg cagtattccg ctgaagaaaa acgttgatga
    32581 cgcgctgaaa aacccgaacg tcaccagcgt agagcatgtg gtggtactga agcgtactgg
    32641 cgggaaaatt gactggcagg aagggcgcga cctgtggtgg cacgacctgg ttgagcaagc
    32701 gagcgatcag caccaggcgg aagagatgaa cgccgaagat ccgctgttta ttctctacac
    32761 ctccggttct accggtaagc caaaaggtgt gctgcatact accggcggtt atctggtgta
    32821 cgcggcgctg acctttaaat atgtctttga ttatcatccg ggtgatatct actggtgcac
    32881 cgccgatgtg ggctgggtga ccggacacag ttacttgctg tacggcccgc tggcctgcgg
    32941 tgcgaccacg ctgatgtttg aaggcgtacc caactggccg acgcctgccc gtatggcgca
    33001 ggtggtggac aagcatcagg tcaatattct ctataccgca cccacggcga tccgcgcgct
    33061 gatggcggaa ggcgataaag cgatcgaggg taccgaccgt tcgtcgctgc gcattctcgg
    33121 ttccgtgggc gagccaatta acccggaagc gtgggagtgg tactggaaaa aaatcggcaa
    33181 cgagaaatgt ccggtggtcg atacctggtg gcagaccgaa actggcggtt tcatgatcac
    33241 gccgctgcct ggcgctaccg agctgaaagc cggttcggca acacgtccgt tcttcggcgt
    33301 gcaaccggcg ctggtcgata acgaaggtaa cccgctggaa ggcgctaccg aaggcagcct
    33361 ggtgatcacc gactcctggc cgggtcaggc gcgtacgctg tttggcgatc acgaacgttt
    33421 tgaacagacc tacttctcca ccttcaaaaa tatgtatttc agcggcgacg gcgcgcgtcg
    33481 cgatgaagat ggctattact ggatcaccgg gcgtgtggac gacgtgctga acgtctccgg
    33541 tcaccgtctg gggacggcag agattgagtc ggcgctggtg gcgcatccga agattgccga
    33601 agccgccgta gtaggtattc cgcacaatat taaaggtcag gcgatctacg cctacgtcac
    33661 gcttaatcac ggggaggaac cgtcaccaga actgtacgca gaagtccgca actgggtgcg
    33721 taaagagatt ggcccgctgg cgacgccaga cgtgctgcac tggaccgact ccctgcctaa
    33781 aacccgctcc ggcaaaatta tgcgccgtat tctgcgcaaa attgcggcgg gcgataccag
    33841 caacctgggc gatacctcga cgcttgccga tcctggcgta gtcgagaagc tgcttgaaga
    33901 gaagcaggct atcgcgatgc catcgtaacc cacaattgcc ggatgcgagt cggtaacggt
    33961 ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcgta
    34021 taacgcctta tccggcctac attcggcaag ggttacccga gcgttaacct tctcccataa
    34081 gggagcggga attaaaacaa tccctacatt acctctggag aatctgtgat gaatggtacg
    34141 atttatcagc ggatagaaga caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg
    34201 tttgccacca tcctgtcgat tattatgctg gcagtttata tcggctttat tttactgatc
    34261 gccttcgcgc ccggctggct gggcacgccg ctgaatccga acaccagcgt cacacgcggt
    34321 attccaattg gtgttggagt gattgtgatc tcctttgttc tcaccggtat ctacatctgg
    34381 cgggcgaacg gcgaattcga ccgtcttaat aatgaagtcc tgcatgaggt acaagcatca
    34441 tgaaaagagt tctgacggcg cttgccgcca cactcccttt cgcagctaac gccgcggatg
    34501 ctattagcgg ggccgtagag cgccagccaa cgaactggca ggcgattatt atgttcctga
    34561 ttttcgtcgt gtttacgctc ggcattacct actgggcatc aaaacgcgta cgttctcgta
    34621 gcgactacta caccgcaggc ggcaatatca ctggcttcca gaacgggctg gcgattgccg
    34681 gggactatat gtccgccgcc tcattcttgg ggatctccgc gctggtgttt acctccggct
    34741 atgacggctt aatttactcg ctgggcttcc tggtgggctg gccgatcatt ttgttcctga
    34801 ttgccgaacg tctgcgtaac ctggggcgct acacctttgc cgatgtggcc tcttaccgtc
    34861 tgaaacaagg gccgattcgt attctttcgg cctgtggttc tctggtggtg gtggcgcttt
    34921 accttatcgc ccagatggtg ggcgcaggta aactgatcga gctgctgttt ggccttaact
    34981 atcacattgc ggtggtgctg gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca
    35041 tgctggcgac cacttgggta cagattatca aagccgtgct gttgctgttc ggtgccagct
    35101 ttatggcctt tatggtgatg aaacacgtcg gctttagctt caacaatctg ttcagtgaag
    35161 cgatggcggt acacccgaaa ggtgtcgaca tcatgaagcc gggcgggctg gtgaaagatc
    35221 cgatctccgc gctctctctg ggtctgggac tgatgtttgg tacggcgggc ttgccgcaca
    35281 ttctgatgcg cttctttaca gtcagcgatg cccgcgaagc acgtaagagc gtgttctacg
    35341 ccaccgggtt tatgggctac ttctatattc tgacctttat tatcggcttc ggcgcgatca
    35401 tgctggttgg tgcgaatccg gaatataaag acgcggcggg ccatctgatt ggtggtaaca
    35461 acatggcggc cgttcacctg gcgaatgcag tgggcggcaa cctgttcctc ggttttattt
    35521 cagcggttgc ttttgccacc attcttgcgg tggttgctgg tctgacgctg gcgggcgcat
    35581 cagcggtttc gcatgacctg tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag
    35641 aactgcgggt atcgaaaatc accgtgctga ttttaggtgt gattgccatt atcctcggcg
    35701 tgctgtttga gaatcagaac atcgccttca tggtaggcct ggcgtttgcc atcgcagcga
    35761 gctgtaactt cccgatcatt ctgctttcta tgtactggtc gaaactgacc actcgtggcg
    35821 cgatgatggg tggctggctg gggctgatta ccgcagtggt actgatgatc ctcggcccga
    35881 cgatttgggt acagatcctt ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc
    35941 tgttctctat cagcgtggca ttcctcggca tctggttctt ctcggcaacc gataactcag
    36001 cggaaggtgc gcgcgagcgt gaactgttcc gtgcacagtt tatccgctcc cagaccggct
    36061 ttggcgttga gcaaggccgc gcacattaat ctctcccctt ccccggtcgc ctgaccgggg
    36121 aatactcttc ctctccagca tgcatcacct tttcccataa tattaaacaa ataaactcat
    36181 taaaaaatga gcgatttttg acagtcgtag aaaatgataa tgcagagaat atgccttttc
    36241 tttcttgtta attata
//
LOCUS       DAZUFB010000041        35519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZUFB010000041 DAZUFB010000000
VERSION     DAZUFB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.041"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..295)
                     /locus_tag="ACHY3M_002270"
     CDS             complement(<1..295)
                     /locus_tag="ACHY3M_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS4317181.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKM"
     gene            complement(455..667)
                     /gene="rpmE"
                     /locus_tag="ACHY3M_002271"
     CDS             complement(455..667)
                     /gene="rpmE"
                     /locus_tag="ACHY3M_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFS4317182.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            870..3068
                     /gene="priA"
                     /locus_tag="ACHY3M_002272"
     CDS             870..3068
                     /gene="priA"
                     /locus_tag="ACHY3M_002272"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4317183.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3224..4249
                     /gene="cytR"
                     /locus_tag="ACHY3M_002273"
     CDS             3224..4249
                     /gene="cytR"
                     /locus_tag="ACHY3M_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFS4317184.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4341..5300
                     /gene="ftsN"
                     /locus_tag="ACHY3M_002274"
     CDS             4341..5300
                     /gene="ftsN"
                     /locus_tag="ACHY3M_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFS4317185.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQPPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5393..5923
                     /gene="hslV"
                     /locus_tag="ACHY3M_002275"
     CDS             5393..5923
                     /gene="hslV"
                     /locus_tag="ACHY3M_002275"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFS4317186.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5933..7264
                     /gene="hslU"
                     /locus_tag="ACHY3M_002276"
     CDS             5933..7264
                     /gene="hslU"
                     /locus_tag="ACHY3M_002276"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFS4317187.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7331..8257
                     /gene="menA"
                     /locus_tag="ACHY3M_002277"
     CDS             7331..8257
                     /gene="menA"
                     /locus_tag="ACHY3M_002277"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4317188.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8350..8835
                     /gene="rraA"
                     /locus_tag="ACHY3M_002278"
     CDS             8350..8835
                     /gene="rraA"
                     /locus_tag="ACHY3M_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFS4317189.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8920..9165)
                     /gene="zapB"
                     /locus_tag="ACHY3M_002279"
     CDS             complement(8920..9165)
                     /gene="zapB"
                     /locus_tag="ACHY3M_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFS4317190.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9590..10435
                     /gene="glpF"
                     /locus_tag="ACHY3M_002280"
     CDS             9590..10435
                     /gene="glpF"
                     /locus_tag="ACHY3M_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFS4317191.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10458..11966
                     /gene="glpK"
                     /locus_tag="ACHY3M_002281"
     CDS             10458..11966
                     /gene="glpK"
                     /locus_tag="ACHY3M_002281"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4317192.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12101..13111
                     /gene="glpX"
                     /locus_tag="ACHY3M_002282"
     CDS             12101..13111
                     /gene="glpX"
                     /locus_tag="ACHY3M_002282"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS4317193.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13208..13954
                     /gene="fpr"
                     /locus_tag="ACHY3M_002283"
     CDS             13208..13954
                     /gene="fpr"
                     /locus_tag="ACHY3M_002283"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFS4317194.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13959..14387)
                     /gene="uspD"
                     /locus_tag="ACHY3M_002284"
     CDS             complement(13959..14387)
                     /gene="uspD"
                     /locus_tag="ACHY3M_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFS4317195.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14414..14713)
                     /gene="yiiS"
                     /locus_tag="ACHY3M_002285"
     CDS             complement(14414..14713)
                     /gene="yiiS"
                     /locus_tag="ACHY3M_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFS4317196.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14925..15365)
                     /gene="yiiR"
                     /locus_tag="ACHY3M_002286"
     CDS             complement(14925..15365)
                     /gene="yiiR"
                     /locus_tag="ACHY3M_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4317197.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15466..16065
                     /gene="yiiQ"
                     /locus_tag="ACHY3M_002287"
     CDS             15466..16065
                     /gene="yiiQ"
                     /locus_tag="ACHY3M_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFS4317198.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16173..16940
                     /gene="tpiA"
                     /locus_tag="ACHY3M_002288"
     CDS             16173..16940
                     /gene="tpiA"
                     /locus_tag="ACHY3M_002288"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4317199.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16995..17750)
                     /gene="cdh"
                     /locus_tag="ACHY3M_002289"
     CDS             complement(16995..17750)
                     /gene="cdh"
                     /locus_tag="ACHY3M_002289"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFS4317200.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAK
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17857..18846)
                     /gene="sbp"
                     /locus_tag="ACHY3M_002290"
     CDS             complement(17857..18846)
                     /gene="sbp"
                     /locus_tag="ACHY3M_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFS4317201.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19165..20127)
                     /gene="pfkA"
                     /locus_tag="ACHY3M_002291"
     CDS             complement(19165..20127)
                     /gene="pfkA"
                     /locus_tag="ACHY3M_002291"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4317202.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20308..21210)
                     /gene="fieF"
                     /locus_tag="ACHY3M_002292"
     CDS             complement(20308..21210)
                     /gene="fieF"
                     /locus_tag="ACHY3M_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFS4317203.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21359..21859)
                     /gene="cpxP"
                     /locus_tag="ACHY3M_002293"
     CDS             complement(21359..21859)
                     /gene="cpxP"
                     /locus_tag="ACHY3M_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFS4317204.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22009..22707
                     /gene="cpxR"
                     /locus_tag="ACHY3M_002294"
     CDS             22009..22707
                     /gene="cpxR"
                     /locus_tag="ACHY3M_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFS4317205.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22704..24077
                     /gene="cpxA"
                     /locus_tag="ACHY3M_002295"
     CDS             22704..24077
                     /gene="cpxA"
                     /locus_tag="ACHY3M_002295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFS4317206.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24183..24857)
                     /gene="yiiM"
                     /locus_tag="ACHY3M_002296"
     CDS             complement(24183..24857)
                     /gene="yiiM"
                     /locus_tag="ACHY3M_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFS4317207.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25006..25989)
                     /gene="kdgT"
                     /locus_tag="ACHY3M_002297"
     CDS             complement(25006..25989)
                     /gene="kdgT"
                     /locus_tag="ACHY3M_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFS4317208.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26249..26869)
                     /gene="sodA"
                     /locus_tag="ACHY3M_002298"
     CDS             complement(26249..26869)
                     /gene="sodA"
                     /locus_tag="ACHY3M_002298"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFS4317209.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27154..28188
                     /gene="rhaT"
                     /locus_tag="ACHY3M_002299"
     CDS             27154..28188
                     /gene="rhaT"
                     /locus_tag="ACHY3M_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFS4317210.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28185..29033)
                     /gene="rhaR"
                     /locus_tag="ACHY3M_002300"
     CDS             complement(28185..29033)
                     /gene="rhaR"
                     /locus_tag="ACHY3M_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFS4317211.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29107..29943)
                     /gene="rhaS"
                     /locus_tag="ACHY3M_002301"
     CDS             complement(29107..29943)
                     /gene="rhaS"
                     /locus_tag="ACHY3M_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFS4317212.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30231..31700
                     /gene="rhaB"
                     /locus_tag="ACHY3M_002302"
     CDS             30231..31700
                     /gene="rhaB"
                     /locus_tag="ACHY3M_002302"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4317213.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31697..32956
                     /gene="rhaA"
                     /locus_tag="ACHY3M_002303"
     CDS             31697..32956
                     /gene="rhaA"
                     /locus_tag="ACHY3M_002303"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4317214.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33039..33863
                     /gene="rhaD"
                     /locus_tag="ACHY3M_002304"
     CDS             33039..33863
                     /gene="rhaD"
                     /locus_tag="ACHY3M_002304"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4317215.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33935..35083
                     /gene="fucO"
                     /locus_tag="ACHY3M_002305"
     CDS             33935..35083
                     /gene="fucO"
                     /locus_tag="ACHY3M_002305"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4317216.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGDSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35080..35394
                     /gene="rhaM"
                     /locus_tag="ACHY3M_002306"
     CDS             35080..35394
                     /gene="rhaM"
                     /locus_tag="ACHY3M_002306"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4317217.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8675 a         9215 c         9242 g         8387 t
ORIGIN      
        1 gcattttcca gccggggccg aggctcccca ccggcgcggg cgtttttgtc cggtaactgc
       61 tgtaggtgcg ggagaggatg aacggcagcg ggccgggcag ggcgatgtcg gtttcaccgg
      121 gaaggacctt tgcaccgagc aggggattga ccggatggcc ggacgtcact ccgccggggc
      181 acaccgaaca ggccacgccg gtgggggcac caatgcgcac cccggctgaa ccctgaacaa
      241 tgctaccgcc atactgcgtc atgtcgccct gacgcgccgc cggttttccg ctcatcgtaa
      301 aatccttttg aaataaataa gaaccagaag cctagcgcca gtcaacaaca ggaaatgata
      361 atgagttaac accccggaat ttttcagaat ttataaaagg caaattacag gcacaaaaaa
      421 agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg atgttgaaac
      481 gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc acgctgtttg ccagtgaaga
      541 acgggtggca cttgctgcac acgtcgaggt tcaggtcatg accaacggtg gagcggattt
      601 tcattacgtt accgcaagag cagctagcag taatttcttc gtatttcggg tgaatatctt
      661 ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa cgccagacac
      721 cacgcgatgt taaaagtata gcttcaatac gatcatttcg tacgaaggcg cgaaatcata
      781 cagaaattaa ccagcgtatg caaactgatc cgcactcttc tacggcaatg tgtatactaa
      841 cccaccgaat ttcaagtcag gatgatgcta tgcccgtagc ccacgttgcc ttgcccgttc
      901 cgcttcctcg tacctttgac tatctgctgc cagaaggcat gacggttaaa gctgggtgtc
      961 gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg gattgtggta tcagttagcg
     1021 atgccagcga actgccgctc aatgagctaa aagcggtagt cgaagtgctg gacgttgagc
     1081 cggtatttac tcactccgtc tggcggttac tgctctgggc ggcagattac tatcatcatc
     1141 cgattggcga tgtgctgttt catgccttgc cgattttact gcgccagggg cggcctgcgg
     1201 cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg gatcttaaca
     1261 gcctgaaacg ttcgccaaag caacaacagg cgctggcggc gttacggcaa ggcaaaatct
     1321 ggcgcgacca ggtcgcggaa cttgaattta atgatgccgc gttgcaggcg ctgcgcaaaa
     1381 aaggtctgtg tgatttagca agtgaaacac cagagtttag cgactggcga acgaactatg
     1441 ccgtttctgg tgagcggttg cgattgaata ccgaacaagc caccgccgtt ggcgcaattc
     1501 atagcgcggc agatactttt tctgcctggc tgctggcggg cgttaccggt tccggtaaaa
     1561 cggaggttta tctcagtgta ctggaaaacg tgctcgctca gggcaaacag gcgctggtga
     1621 tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg ttttcgtgaa cgttttaatg
     1681 cccccgtgga agttctgcat tccggcctga acgacagcga gcggctttcg gcgtggttga
     1741 aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg ctccgcgctg tttacgccgt
     1801 ttaaaaatct cggcgtgatt gtcattgatg aagagcacga cagctcctac aagcagcagg
     1861 aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg tgcgcatagc gagcaaatcc
     1921 cgattattct tggctccgca acgcccgcgc tggaaacgtt atgcaacgtc cagcagaaaa
     1981 aataccgcct gctgcgcctg acccgtcggg cagggaatgc gcgtccggca attcaacatg
     2041 tgctggattt aaaaggtcag aaggtgcagg caggtctggc tccggcgtta atcacccgta
     2101 tgcgccagca tttacaggcc aacaatcagg ttatcctctt tcttaatcgt cggggttttg
     2161 cgcctgcgct gctgtgccac gactgcggct ggatagccga atgcccacgt tgcgatcact
     2221 actacacgct gcatcaggcg caacagcatt tgcgctgtca ccactgtgac agtcagcgtc
     2281 cggtgccgcg ccagtgccct tcctgcggtt ccacgcacct ggtccccgtg gggctgggca
     2341 ccgaacagct tgaacagacg cttgcgccga tgttccccgg cgtgcccatt tcacgtatcg
     2401 accgcgatac caccagccgc aaaggggcgc tggaacagca actggcggaa gtgcatcgcg
     2461 gcggcgcgag gatcttgatt ggcacgcaaa tgctggcgaa aggtcaccac tttccggatg
     2521 tgacgctggt ggcgttactg gatgtggacg gcgcgctgtt ttctgccgat tttcgctcgg
     2581 cagaacgttt cgcccagctt tacacccagg tcgccggtcg tgccgggcgt gcgggtaaac
     2641 agggcgaagt ggtgctgcaa acgcaccatc cggaacatcc tctgttgcaa acgttgctct
     2701 ataaaggcta cgacgccttt gccgaacagg cgctggctga gcgacgcatg atgcagctgc
     2761 cgccgtggac cagccatgtg attgtgcgtg cggaagatca taacaatcag catgcgccgc
     2821 tgttcctgca acaactgcgt aatctgatcc tttccagccc gctggcagac gacaaactgt
     2881 gggttctcgg cccggttccg gctctggcac ctaagcgtgg tggccgctgg cgctggcaga
     2941 tcttgttgca gcacccttcc cgcgtgcgct tgcaacacat cattagtggt acgctggcgc
     3001 ttattaatac aataccggat tcccgcaagg tgaaatgggt gttggatgtc gatcccatcg
     3061 aaggctaatg cgcaaacgtt gcgaggcgga tcgaaaaatt caatattcat cacacttttc
     3121 atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa aatgttgccg atgtgaagta
     3181 aacatggatg tagtacgcct gacgtgccag gcgaggagtg agtgtgaaag cgaagaagca
     3241 ggaaactgcc gcgaccatga aagacgttgc cctcaaggca aaagtctcta cagcgactgt
     3301 ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc acccgtaatc gggttgaaaa
     3361 agcggcccgg gaagtgggtt atttaccgca gcctatgggg cgcaacgtca agcgtaatga
     3421 atcccgcacc attctggtga ttgtcccgga tatctgcgat cccttcttta gcgaaattat
     3481 tcgcggtatc gaagttacgg cggcaaatca cggatatctg gtgctgattg gcgactgtgc
     3541 gcatcaaaat cagcaggaaa aaacctttat cgatttgatc atcaccaagc aaattgatgg
     3601 catgttgctg ctgggttcaa ggctgccgtt tgatgccagc attgaggaac agcgtaatct
     3661 gccgccgatg gtgatggcga acgaattcgc accggaactg gagctaccta cagttcatat
     3721 cgacaatctg accgccgcat ttgatgcagt aaattatcta tatgagcaag ggcataaacg
     3781 gattggctgt atagccggtc ccgaagagat gccgctgtgt cactaccgcc tgcaaggtta
     3841 cgttcaggcg ctgcgtcgct gcggcattat ggttgatccg caatacatcg cccgtggcga
     3901 cttcaccttc gaagccggaa gcaaagcgat gcagcagctg cttgatcttc cacaaccgcc
     3961 tactgctgtc ttctgccata gcgatgtgat ggcgctcggc gcactttctc aggcaaaacg
     4021 ccaggggctg aaagtcccgg aagacctttc cataatcggt tttgataaca tcgacctgac
     4081 gcaattttgt gatccgccgc tgacaaccat cgcgcagccg cgttacgaaa tcggtcggga
     4141 agctatgctg ttattgcttg atcaaatgca ggggcaacac gttggcagtg gctctcgttt
     4201 aatggactgc gaacttatca tccggggatc aacacgcgcg ttaccttaaa gtaaaccttt
     4261 aagactgccg tgactggtca aaggcccgcc gcttaagtaa catggcgggc tgacgaacga
     4321 ataaatacag cgaaacgata gtggcacaac gagattatgt acgccgcagc caaccggcac
     4381 cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg aaatctgcct gcggtttctc
     4441 ccgctatggt cgctattgct gccgccgttc ttgtgacctt tatcggtggt ctgtacttca
     4501 ttacgcatca caagaaagaa gagtccgaga cgctgcaaag ccagaaagtg accggtaacg
     4561 gactaccacc aaaaccagaa gaacgctggc gctacattaa agagctggaa agtcgccagc
     4621 cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga agtgaaaacg ccggagcaac
     4681 tgacaccaga acaacgtcag cttcttgaac aaatgcaggc tgatatgcgc cagcagccaa
     4741 cgcagctggt tgaagttccg tggaatgaac agacgccgga acagcgtcag caaacgctac
     4801 aacgccagcg tcaggcgcag caattagcgg agcagcaacg cctggctcag cagtcgcgta
     4861 cgactgaaca aagctggcag cagcagacgc gtacgtcgca agccgcgccg gtacaggcgc
     4921 aaccgcgcca gtctaaaccg gcttctactc agcagccgta ccaggatctg ctgcaacccc
     4981 ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc gccggttgct cgtgccgctg
     5041 acgcgccaaa accgacggcg gagaaaaaag acgaacgccg ctggatggtg cagtgcggtt
     5101 cgttcagagg cgcggaacag gcagagacgg tacgtgctca gctggcgttc gaaggatttg
     5161 actcgaaaat caccaccaac aatggctgga atcgtgtggt cattggcccg gtgaaaggca
     5221 aagagaacgc agacagcacc ctcaatcggt tgaagatggc gggtcataca aactgcattc
     5281 ggctcgccgc cgggggttga aaccctcaaa atccccccca tctataattg cattatgccc
     5341 cgtacttttg tacggggttt gtactctgta ttcgtaacca aggggtcagc tcgtgacaac
     5401 tatagtaagc gtacgccgta acggccatgt ggtcatcgct ggtgatggtc aggccacgtt
     5461 gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc cgtctgtaca acgacaaagt
     5521 catcgcgggc tttgcgggcg gtactgcgga tgcttttacg ctgttcgaac tgtttgaacg
     5581 taaactggaa atgcatcagg gccatctggt caaagccgcc gttgagctgg cgaaagactg
     5641 gcgtaccgat cgcatgctgc gcaaacttga agcactgctg gcagtcgcgg atgaaactgc
     5701 atcgcttatc atcaccggta acggtgacgt ggtgcagcca gaaaacgatc ttattgctat
     5761 cggctccggc ggcccttacg cccaggctgc ggcgcgtgca ctgttagaaa atactgaact
     5821 gagtgctcgt gaaattgctg aaaaggcgtt ggatattgca ggcgacattt gcatctatac
     5881 caaccatttc cacaccatcg aagaattaag ctacaaagcg taaggatctc ccatgtctga
     5941 aatgacccca cgcgaaatcg tcagcgaact ggataagcac atcatcggcc aggacaacgc
     6001 caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt cgcatgcagc tcaacgaaga
     6061 gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc ggcccgaccg gtgtcggtaa
     6121 aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg ccgttcatca aagttgaagc
     6181 gaccaaattc accgaagtag gctacgtcgg taaggaagtg gattctatta ttcgcgatct
     6241 gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc gagaaaaacc gttatcgcgc
     6301 tgaagaactg gcagaagaac gtattctcga cgtgctgatc ccacctgcta aaaacaactg
     6361 gggacagacc gaacagcagc aggaaccgtc cgctgctcgt caggcattcc gcaaaaaact
     6421 gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat cttgccgcag caccgatggg
     6481 cgttgaaatt atggctcctc caggcatgga agagatgacc agccagctgc agtccatgtt
     6541 ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg aaaatcaaag acgccatgaa
     6601 gctgctgatt gaagaagaag cggcgaaact ggtgaacccg gaagagctga agcaagacgc
     6661 tatcgacgct gttgagcagc acgggatcgt gtttatcgac gaaatcgaca aaatctgtaa
     6721 gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc gttcagcgtg acctgctgcc
     6781 gctggtagaa ggttgcaccg tttccaccaa acacgggatg gtcaaaactg accacattct
     6841 gtttatcgct tctggcgcgt tccagattgc gaaaccgtct gacctaatcc cggaactgca
     6901 aggtcgtctg ccaatccgcg ttgaactgca ggcactgacc accagcgact tcgagcgtat
     6961 tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa gcactgatgg cgactgaagg
     7021 cgtaaatatc gagtttaccg actccggtat taagcgcatc gcggaagcgg catggcaggt
     7081 gaacgaatct accgaaaaca tcggtgctcg tcgtttacac actgttctgg agcgtttaat
     7141 ggaagagatt tcctacgacg ccagcgattt aagcggtcaa aatatcacta ttgacgcaga
     7201 ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa gatctgagcc gttttatcct
     7261 ataatcgcgt tcaatcattt tcatcattgt ttgatggggc tgaaaggccc catttttatt
     7321 ggcgcgtatt atgactgaac aacaaattag ccgaactcag gcgtggctgg aaagtttacg
     7381 acctaaaacc ctccccctcg cctttgctgc aattatcgtc gggacggcgc tggcatggtg
     7441 gcaaggtcac ttcgatccgc tggtcgccct gctggcacta attaccgccg ggctattaca
     7501 gatcctttct aacctcgcca atgattacgg cgatgcggta aaaggcagcg ataaacctga
     7561 ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt acccagcaag agatgaaacg
     7621 ggcgctcatt attaccgtcg tgctcatctg tctctccggg ttggcactgg ttgcagtggc
     7681 atgccatacg ctggccgatt ttgtcggttt cctgattctt ggcgggttgt cgatcattgc
     7741 cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat atcggtctgg gtgatatttc
     7801 cgtactggtt ttctttggct ggttgagtgt catggggagc tggtatttac aggctcatac
     7861 attgattccg gcactgatcc ttccggcgac cgcatgcggc ctgctggcaa cggcagtact
     7921 gaatattaat aacctgcgtg atatcaatag cgaccgcgaa aatggcaaaa acacgctggt
     7981 ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc tgcctgctga tgggctcgct
     8041 ggtgtgtctg gcgctgttta atctcttttc gctgcatagc ctgtggggct ggctgttcct
     8101 gctggcggca ccattactgg tgaagcaagc ccgttatgtg atgcgggaaa tggacccggt
     8161 ggcgatgcga ccaatgctgg aacgtactgt caagggagcg ttactgacta acctgctgtt
     8221 tgttttaggg atattcctaa gccagtgggc agcataactg acaaatatca attaacaatt
     8281 gatgattttg ccaacagccc acatagcgcg atatactgaa aattctcgca gcaactgaat
     8341 gttaagccta tgaaatacga tacttccgag ctttgtgaca tctatcaaga agatgttaac
     8401 gtcgtggaac cgctgttctc caactttggc ggacgggcgt cgtttggcgg acaaataatc
     8461 acggtaaaat gtttcgagga caacgggttg ctgtacgatc tgctcgaaca gaatggccgt
     8521 ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc gcgcactggt cgatgctgaa
     8581 ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg tcatttacgg cgcggtgcgt
     8641 caggtagatg acctggaaga gttggatatc ggcatccagg cgatggcggc aattccggtt
     8701 ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg tcaattttgg cggtgtcacc
     8761 ttcttctccg gcgaccatct ttatgccgac aataccggga ttattctttc tgaagatccg
     8821 ctggatattg aatgatagaa aaggcaccgc aaggtgcctt ttttctgcgt tacctgttgg
     8881 cctacacagt aaagaaatta cgcggaagat gaagcgtaat cagacctctt ccatgcgacc
     8941 cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc
     9001 acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt
     9061 tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg
     9121 tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg
     9181 caaggcgttg atggataaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga
     9241 gcgcacttta gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct
     9301 tgcagattac ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc
     9361 gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag
     9421 atggaataaa tggcgcgata acgctcattt tatgacaaag cacacacatt ttaagttcga
     9481 tatttctcgt ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg
     9541 tccgtgactt tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac
     9601 atcaaccttg aaaggccagt gcattgctga attcctcggt accgggttgt tgattttctt
     9661 cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat
     9721 cagtgtcatt tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg
     9781 cgcgcatctt aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg
     9841 caaagttatt ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt
     9901 ttacgggctt tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg
     9961 cagcgttgaa agtgttgatc tggctggcac tttctctact taccctaatc ctcatatcaa
    10021 ttttgtgcag gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct
    10081 ggcgttaacg gatgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg
    10141 tctactgatt gcggtcattg gcgcatctat gggcccattg acaggttttg ccatgaaccc
    10201 agcgcgtgac ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt
    10261 taccggcggc agagacattc cttacttcct ggtgccgctt ttcggcccta tcgttggcgc
    10321 gattgtaggt gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg
    10381 tgttgtggaa gaaaaggaaa ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga
    10441 ctacgggaca attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca
    10501 ccagctcccg cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg
    10561 aatttgagca aatctaccca aaaccaggtt gggtagaaca cgacccaatg gaaatctggg
    10621 ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa
    10681 ttgcagctat cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg
    10741 gcaagcctat ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc
    10801 atttaaaacg tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc
    10861 cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc
    10921 gtgcacgtcg tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga
    10981 ctcagggccg tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca
    11041 tccataccct ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc
    11101 tgccagaagt gcgtcgttct tccgaagtat acggtcagac taacattggc ggcaaaggcg
    11161 gcacgcgtat tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt
    11221 tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga
    11281 acactggcga gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc
    11341 cgactggcga agtgaactat gcgttggaag gtgcggtgtt tatggcaggc gcatccattc
    11401 agtggctgcg cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca
    11461 ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc atttaccggg ctgggtgcgc
    11521 cgtactggga cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta
    11581 accacattat acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag
    11641 cgatgcaggc cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag
    11701 caaacaattt cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg
    11761 aagtgcgcga agtcaccgca ttgggtgcgg cctatctcgc aggcctggcg gttggcttct
    11821 ggcagaacct cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca
    11881 tcgaaaccac tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga
    11941 tggcgtggga agaacacgac gaataatgta aatgccgaat gaagcgttta tgccgcatcc
    12001 ggtagtcccg aaacgtgcgg gggcaacccc gcacacatca ataatccctc ccttcccctg
    12061 tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat
    12121 cgaattttcc cgcgtcaccg aatcagcggc gctggctggc tacaaatggt taggacgcgg
    12181 cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt
    12241 caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac cgatgctcta
    12301 cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg ttgatccgat
    12361 tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg
    12421 cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga ttgtcgggcc
    12481 gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc gcaatgtagc
    12541 ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta aaccacgcca
    12601 cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggatgg
    12661 cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg
    12721 tattggtggc gcgccggaag gcgtagtttc tgcggcggtg atccgcgcat tagatggcga
    12781 catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg
    12841 cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg gtaaagtatt
    12901 gcgcctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg gtattaccaa
    12961 aggcgatctg ctggaaggca ttagccgcaa aggcaatatc gcgactaccg aaacgctgct
    13021 gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc tggatcgcaa
    13081 agacccggaa atgcaggtgc acatcctctg attgatttga tcgattgagc cttccagtcc
    13141 ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat caaaacagga
    13201 gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc
    13261 cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc aattcaccaa
    13321 gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc
    13381 cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg
    13441 attggcggca ctgaaaccag gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt
    13501 tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg gtacagcgat
    13561 tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca aaaatctggt
    13621 cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact
    13681 ggaaaaacgc tacgaaggga aactgcgcat tcagacggtg gtcagtcggg aaacggcagc
    13741 ggggtcgctc accgggcgga taccggcgtt aattgaaagt ggggaactgg aaagcgcgat
    13801 tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt
    13861 gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg
    13921 accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg
    13981 atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat caaacggttg
    14041 ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc
    14101 agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata
    14161 tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct
    14221 tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt
    14281 aacgtcaggt gagcgtcatt atgtctggcg agctccagag ctttattcac cagtaaggca
    14341 tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc
    14401 taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct
    14461 ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttgt
    14521 aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg
    14581 tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt
    14641 tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta
    14701 cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat
    14761 aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga
    14821 tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa
    14881 ataacaatcc cgaaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta
    14941 ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc gccttgtgtg
    15001 ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc
    15061 cccaccgccc attgccagac acccggtaaa atcgcccagt tacccgccag cagcatccac
    15121 gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaaca
    15181 gttactgccg ctgttggcca aagcaagcac acaaggcaaa acgccgcggt ctgaatatcg
    15241 agtaaatttt tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc
    15301 caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg
    15361 gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac
    15421 agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt
    15481 acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca
    15541 ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctcc
    15601 atcagccagt ttcgagaaga ctttaacagc cagaatccta gcctgccact gaacgaattt
    15661 cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag taaaattaac
    15721 gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa
    15781 atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa agctcaggaa
    15841 tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa
    15901 aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga gacagaaggt
    15961 gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg
    16021 attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct
    16081 ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt
    16141 cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact
    16201 ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc
    16261 tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg
    16321 cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc
    16381 tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc ggcgctcagt
    16441 acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac gaactgattg
    16501 cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc ggttctgtgc atcggtgaaa
    16561 ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg cgcacgtcag atcgacgcgg
    16621 tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac gaacctgtat
    16681 gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt cacaaattca
    16741 tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc attcagtacg
    16801 gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagat atcgacggcg
    16861 cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt aaagctgcag
    16921 aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc acttactttc
    16981 cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc ctcggctgaa
    17041 gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga attatcactc
    17101 tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg cacttcttct
    17161 gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg
    17221 tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat tttcgccaga
    17281 tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat atgaaaatgg
    17341 ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc
    17401 tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc
    17461 gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt tggcatcaac
    17521 agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt gggtttgact
    17581 tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc ctcaaggaca
    17641 atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc
    17701 acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc
    17761 ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc
    17821 gagaaaccac cgggtttgat aattatcctg gggaaatcag cgtttgctga tctgatcgaa
    17881 cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc
    17941 aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta cttcagcgtc
    18001 gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt
    18061 caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat cgaccaccga
    18121 cacggttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt tatctttccc
    18181 cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat cgccaatccc
    18241 gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt cgacgttttt
    18301 atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc
    18361 gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg gattaggcgt
    18421 gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct tcggattgcc
    18481 cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt
    18541 gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc
    18601 cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt tacctgaacc
    18661 accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt gggcgctgaa
    18721 tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg
    18781 aatatccttt gccataacgt tggttgccgc cagcaaaaat gttaacccta cgccccactt
    18841 gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac
    18901 caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt gggggttaaa
    18961 aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat gccggatgcg
    19021 gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc agaattcatg
    19081 taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt tcagggtaaa
    19141 ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca gccagtcacc
    19201 tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa ccagctgttc
    19261 gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga tagcgtaagc
    19321 gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc gctggatgtg
    19381 tcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat gcgccagttc
    19441 gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac ctttcgcgat
    19501 acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg gaaccacaac
    19561 gaattcacag ccaccggcaa tggccgcagc caacgtcaga tcgccacaat aacggcccat
    19621 cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac ggtcgatcgc
    19681 ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc ctttgatgtc
    19741 gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca gacgcattgc
    19801 acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgatcccac gttttttcag
    19861 gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaagc gcgcagaacc
    19921 gaggaacgta ccgccacggt tgatcatgtc agaaacgctg taacggtcta gctgtaccat
    19981 acggtcttca tacagaccca gatagccgtc ataaatgccc attacttcca gaccttctgt
    20041 cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat caccgccgct
    20101 tgtcaacaca ccgattttct taatcatgac tacctctgaa ctttggaatg caaaataaaa
    20161 tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca aatagtataa
    20221 caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt atacacaaaa
    20281 tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac cctccctggg
    20341 tacgacggaa caggggtcct gatggataat tacatccgat cccggaaaac gccgtaaaat
    20401 agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag agtcttccat
    20461 ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa gatcgtgagc
    20521 gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt cctcatcagg
    20581 caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg cgctatataa
    20641 aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc cgtaccagga
    20701 caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt aatgtagcat
    20761 atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caacgctgaa acgagacaag
    20821 gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg gatctgtcat
    20881 cggtgttgga gatatcagat gttgaatacc cgtcaaaaac aggaatagtg ccgaaccgga
    20941 gataaacata ctttgcgcca gcgccgcgag ggactctgct ttaccgtgac caaacgagtg
    21001 attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca acgacgcgcc
    21061 gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg tataccacca
    21121 tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa tcgccgcccg
    21181 actgaccagc cgtccataag attgattcat aaatactccc gctatcaact gacgctagta
    21241 taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga taaaaaaaaa
    21301 ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac agggtttact
    21361 actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt tgccattgcg
    21421 tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc gcttgctgct
    21481 ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc tgacgagcaa
    21541 tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca aaattttctg
    21601 cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc
    21661 gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa cttatgccgt
    21721 cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc
    21781 ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag gccatgacgg
    21841 cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga
    21901 gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg
    21961 attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc
    22021 ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa
    22081 ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc
    22141 attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa
    22201 gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt
    22261 gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat
    22321 cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag
    22381 caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc
    22441 cgtcaggaag ccagtttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg
    22501 ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa
    22561 gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg
    22621 cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc
    22681 ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct
    22741 tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac
    22801 gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg
    22861 tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc
    22921 gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc
    22981 gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg
    23041 ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct
    23101 ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc
    23161 aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt
    23221 tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc
    23281 tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag
    23341 cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg
    23401 agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc
    23461 cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt gaaagcaagg
    23521 aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg
    23581 tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt
    23641 tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga
    23701 cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg
    23761 cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct
    23821 ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc
    23881 cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg
    23941 aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg
    24001 gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc
    24061 tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg
    24121 gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg
    24181 ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt
    24241 tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga
    24301 tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg
    24361 acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta
    24421 tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa
    24481 tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc
    24541 cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt tgagagattt
    24601 tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg cacccagtag
    24661 agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg aacctttttc
    24721 tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg
    24781 attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca
    24841 tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta
    24901 ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc
    24961 ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc
    25021 taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag aggtaagaat
    25081 tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg ctgccggagc
    25141 catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta ccgcggcccc
    25201 tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc
    25261 gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa
    25321 accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg
    25381 aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag
    25441 ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc
    25501 aatccctgca gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa
    25561 cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc
    25621 gccattggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc
    25681 aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac
    25741 ggcaattttt gttaccacca gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa
    25801 ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg taccggtaat
    25861 catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag
    25921 tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa tcgagcgttt
    25981 tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtca ttcctcaatg
    26041 gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa agtgatcaaa
    26101 accacatcaa ttgaaacgct gttttatttt tatcggatca ttgtttttat tttgaaattg
    26161 atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata tgcggcctat
    26221 acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt gccgctgctt
    26281 cgtcccagtt caccacgttc cagaactctt taatgtagtc cggacggcgg ttctggaatt
    26341 tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg gaagcgccag
    26401 aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc gccagtttat
    26461 cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc gctttttcaa
    26521 attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct ttcaggtcac
    26581 cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta gcgtgaccgc
    26641 cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt ttggtgatca
    26701 gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt
    26761 ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg
    26821 gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata ttcatctcca
    26881 gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggttcatta tagttaatta
    26941 aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa
    27001 aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa
    27061 cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga
    27121 tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata
    27181 ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta
    27241 aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg gattattctg
    27301 ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag ctcgtttagt
    27361 ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac
    27421 tacggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt
    27481 acgttgattg tcggtacact gatgacgcca attatcaacg gcaatttcga tgtgctgatt
    27541 aacaccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggcgtaggg
    27601 attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc cgaagagttc
    27661 aatctgaaaa aagggctggt gctggcggtt atgtgcggca ttttctctgc cgggatgtcc
    27721 tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg cgtcgatcca
    27781 ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat cattaacctc
    27841 ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg
    27901 ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg gttgatgtgg
    27961 tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac
    28021 atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cggactggtg
    28081 ctgaaagagt ggagcaatgc aggacgccgt ccggtaacgg tgttgagcct cggttgtgtg
    28141 gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa
    28201 ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa
    28261 tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc
    28321 tggagaagat attgcgcatg acacactctg acctgtcgca gatattgatt gatggtcatt
    28381 ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa
    28441 aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc
    28501 agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc
    28561 aacatcgcca actgcccgaa cagcaactct gccatttcgt tagcaaacgg cacatgctga
    28621 ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct
    28681 aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgcccc ctgccagtca
    28741 agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg
    28801 gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga
    28861 taagggcgat cgttgagtac atgcaggcca ttaccgcgcc agacaatcac cagctcacaa
    28921 aaatcatgtg tatgttcagc aaagacatgt tgcggataac ggtcagccac agcgactgcc
    28981 tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct
    29041 acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga
    29101 ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata tcacgcggtg accagttaaa
    29161 ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccgcaat gataggcgat
    29221 gtcagtaacg ctggcctcac tgtggcgtag cagatgtcgg gccttcatca gtcgcaggcg
    29281 gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgttcg
    29341 cagtgaaaga gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc
    29401 ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa
    29461 actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga
    29521 gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg
    29581 caatacgctg tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag
    29641 caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt
    29701 cagacacaga ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac
    29761 cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc
    29821 gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct gagggagccg
    29881 gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt
    29941 catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta
    30001 ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa
    30061 ttttcaggaa atgcggtgag catcacatca ccacaatgaa gcaaattgtg aacatcatca
    30121 cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcacga
    30181 attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc
    30241 gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg
    30301 agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaaaaat gggctgcata
    30361 gccagaacgg ttatgtcacc tgggatgtgg atagcctgga aagtgccatt cgccttggct
    30421 taaacaaagt gtgtgaggaa gggattcgta tcgatagcat tgggattgat acctggggcg
    30481 tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg
    30541 atagccgcac caatggccta atggcgcagg cacagcaaca actcggcaaa cgcgatattt
    30601 atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcagttg cgtgcgctga
    30661 cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact
    30721 tcagttatcg cctgaccggc aagatgaact gggagtacac caatgccacc accacacaac
    30781 tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca
    30841 aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc
    30901 agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg
    30961 cctcgccgtt aaacggttca cgcgccgctt atctctcttc tggcacctgg tcattgatgg
    31021 gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaac atcaccaatg
    31081 aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc
    31141 agcgagtgct tcaggaacgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg
    31201 cacttccggc ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgagg
    31261 cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg
    31321 atgctgaact ggcgcgctgt attttcgaca gtctggcgct gctgtatgcc gatgtgttgc
    31381 atgagctggc gcagctgcgc ggtgaagatt tctcgcaact gcatattgtc ggcggcggct
    31441 gccagaacac gctgctcaac cagctatgcg ccgatgcctg cggtattcgg gtgatcgccg
    31501 ggcctgttga agcctcaacg ctcggcaata tcggcatcca gttaatgacg ctggatgaac
    31561 tcaacaatgt ggataatttc cgtcaggtcg tcagcaccac cgcgaatctg accaccttta
    31621 cccctaatcc tgacagtgaa attgcccact atgtggcgca gattcactct acacgacaga
    31681 caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg cgaaacagcg
    31741 tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc caacttgatc gtttacccgt
    31801 ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg aaggttcgct
    31861 gaccggtggg attcaagcta ctggtaatta tccgggcaaa gcgcgtaatg ccagtgagct
    31921 acgtaccgat ctggaacagg ctatgcggct gattccgggg ccgaaacggc ttaatttaca
    31981 tgccatctat ctggaatcag acacgccagt ctcgcgcgac cagatcaaac ctgagcactt
    32041 caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta acccctcctg
    32101 tttttcgcat ccgctaagcg ccgatggctt tacgctttcc catgccgacg acagcattcg
    32161 ccagttctgg attgatcact gcaaagccag ccgtcgcgtt tcggcctatt ttggcgagca
    32221 actcggcaca ccatcggtga tgaacatctg gatcccggat ggtatgaaag atatcaccgt
    32281 tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg gatgaggtga tcagcgagaa
    32341 gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca ttggcgcgga
    32401 gagctacacg gttggctcca atgagtttta cctggggtat gccaccagcc gccagacggc
    32461 gctgtgcctg gacgccgggc acttccaccc gactgaagtg atttccgaca agatttccgc
    32521 cgccatgctg tatgtgccgc agttgctgct gcacgtcagt cgtccggttc gctgggacag
    32581 cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt gccagtgaga ttgtgcgtca
    32641 cgatctgttt gaccgggtgc atatcggcct tgacttcttc gatgcctcta tcaaccgcat
    32701 tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc ctgctgcgtg cgttgctgga
    32761 acctaccgcc gagctgcgca agttggaagc ggcgggcgat tacactgcgc gtctggcact
    32821 gctggaagag cagaaatcgt tgccgtggca ggcggtatgg gaaatgtatt gtcagcgtca
    32881 cgatacccca gcgggtagcg aatggctgga gagcgtgcgg gcttatgaga aagaaatttt
    32941 gagtcagcgt gggtaaacac tgccagatgc ggcacgagcg ccttatccgg cctacagatc
    33001 gtgcatctga caacacccca tttacaggaa caccgaacat gcaaaacatt actcagtcct
    33061 ggtttgtcca gggaatgatc aaagccacca ccgacgcctg gctgaaaggc tgggacgagc
    33121 gcaacggcgg caacctgacg ctacgcctgg atgacgctga catcgcaccg tatcacgaca
    33181 atttccacca acaaccgcgt tatatcccgc tcagccaacc catgccttta ctggcaaata
    33241 caccgtttat cgtcaccggc tcgggcaaat tcttccgtaa cgtccagctt gatcctgcgg
    33301 ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt ctctgggggt
    33361 taaccaacga ggccgttcca acttctgaac ttccggcaca ctttctctcc cattgcgaac
    33421 gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccatgcc accaacctga
    33481 tcgccctcac ctatgtactg gaaaacgaca cggcggtctt cactcgccaa ctgtgggaag
    33541 gcagcaccga gtgcctggtg gtattcccgg atggcgttgg cattctgccg tggatggtgc
    33601 ccggcacgga cgaaatcggt caggcgaccg cacaagaaat gcaaaaacat tcgctggtgt
    33661 tgtggccctt ccacggtgtg ttcggttccg gaccgacact ggatgaagcc tttggtttga
    33721 tagataccgc agagaaatca gcggaggtgt tagtcaaaat ctactcgatg ggcggtatga
    33781 agcagaccat tacccgtgag gagctgattg cactcggtga gcgttttggc gttacgccac
    33841 tggccagtgc gctggcactt taatatccca acagatatcc ggcggtgtgg gaaacgtcgc
    33901 cggacagatg gatttgaact taaggagagt cattatgagt tttatgttgg cattaccgaa
    33961 aattagcctg catggtgctg gggcaattgg cgatatggta aatctggttg cgcataaaca
    34021 gtggggaaaa gcactgatcg ttaccgatgg gcaactggtt aagttgggat tactggacag
    34081 cctgtttatc gcactggatc aatacgaaat gtcttatcac ttatttgatg aggtatttcc
    34141 taaccccact gaagaactgg tgcaaaaagg ctacgctgca tttcaggatg ccgcatgtga
    34201 ctacattatt gctttcggtg gcgatagccc aattgatact gcaaaagccg taaaaatcct
    34261 taccgctaat cctgcgcctt ctacggcgta ctctggtgtt ggtaaagtga caaatcccgg
    34321 cgtaccgctg gtggcaatca atacgactgc cggaacagct gctgagatga ccagcaatgc
    34381 ggtgatcatc gacaccgcgc ggcaggtaaa agaggtcatt atcgacccga atattattcc
    34441 cgatattgcg gtggatgatg ccagtgtaat gctggagatc ccggcctcgg ttaccgccgc
    34501 cacgggtatg gatgcactga ctcacgcagt tgaagcttat gtttctgttg gtgcacatcc
    34561 attaaccgac gctaacgcac tggaagctat ccgtttaatt acgctgtggc tgccaaaagc
    34621 ggtagatgat ggtcataatc tggaagctcg tgaacaaatg gcattcgccc agtacctggc
    34681 gggtatggca tttaacagtg ctggtctggg ccttgttcat gcacttgcgc atcagcccgg
    34741 cgccacccac aacctgccgc atggtgtgtg caatgctatc ttgttgccag ttattgaaaa
    34801 ctttaaccgc ccgaatgcca tcgcacgctt tgcccgcatt gctcaggcaa tgggtgtcga
    34861 tacgcgtgat atgagtgaag aagcggcaag tactgaagca attaacgcta tccgcgcatt
    34921 aagccatcgc gtgggtattc cggcaggatt cagccagctt ggcatcacca aagaagatat
    34981 tgaaggctgg ttagataaag ccctggctga cccgtgtgtg ccatgtaacc cgcgtaccgc
    35041 cagccgcgac gaggtgcgcg agctgtatct ggaggcatta tgatccgtaa ggcatttgtg
    35101 atgcaggtta accccgacgc tcacgaagag tatcaacgtc ggcataaccc catctggccg
    35161 gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa
    35221 gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagagcgctg gaatgcggtt
    35281 gccagtactg atgtttgcca acgttggtgg aaatatatga ccgacgtcat gcccgcgaac
    35341 ccggataaca gcccggtgag tagcgaactg caagaagtgt tttacctgcc gtaattgcct
    35401 aatgtgccgg agacgcctcc ggtacaatat ttttaatttc gatttgaaaa ataaacatat
    35461 tacaccttca gttaatatat ctcggtaact tatatttccc cgatgcattg tgagtaaat
//
LOCUS       DAZUFB010000042        34976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZUFB010000042 DAZUFB010000000
VERSION     DAZUFB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.042"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(234..377)
                     /gene="ypaB"
                     /locus_tag="ACHY3M_002307"
     CDS             complement(234..377)
                     /gene="ypaB"
                     /locus_tag="ACHY3M_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFS4317218.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            505..2790
                     /gene="nrdA"
                     /locus_tag="ACHY3M_002308"
     CDS             505..2790
                     /gene="nrdA"
                     /locus_tag="ACHY3M_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4317219.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2879..4009
                     /gene="nrdB"
                     /locus_tag="ACHY3M_002309"
     CDS             2879..4009
                     /gene="nrdB"
                     /locus_tag="ACHY3M_002309"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4317220.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4009..4263
                     /gene="yfaE"
                     /locus_tag="ACHY3M_002310"
     CDS             4009..4263
                     /gene="yfaE"
                     /locus_tag="ACHY3M_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFS4317221.1"
                     /translation="MASVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4317..4967)
                     /gene="inaA"
                     /locus_tag="ACHY3M_002311"
     CDS             complement(4317..4967)
                     /gene="inaA"
                     /locus_tag="ACHY3M_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFS4317222.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5047..5151)
                     /locus_tag="ACHY3M_002312"
                     /pseudo
     CDS             complement(5047..5151)
                     /locus_tag="ACHY3M_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5149..5388
                     /locus_tag="ACHY3M_002313"
                     /pseudo
     CDS             5149..5388
                     /locus_tag="ACHY3M_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5430..6506)
                     /gene="glpQ"
                     /locus_tag="ACHY3M_002314"
     CDS             complement(5430..6506)
                     /gene="glpQ"
                     /locus_tag="ACHY3M_002314"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4317223.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6511..7869)
                     /gene="glpT"
                     /locus_tag="ACHY3M_002315"
     CDS             complement(6511..7869)
                     /gene="glpT"
                     /locus_tag="ACHY3M_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFS4317224.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8142..9770
                     /gene="glpA"
                     /locus_tag="ACHY3M_002316"
     CDS             8142..9770
                     /gene="glpA"
                     /locus_tag="ACHY3M_002316"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFS4317225.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9760..11019
                     /gene="glpB"
                     /locus_tag="ACHY3M_002317"
     CDS             9760..11019
                     /gene="glpB"
                     /locus_tag="ACHY3M_002317"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFS4317226.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11016..12206
                     /gene="glpC"
                     /locus_tag="ACHY3M_002318"
     CDS             11016..12206
                     /gene="glpC"
                     /locus_tag="ACHY3M_002318"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFS4317227.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12400..13302
                     /gene="rpnE"
                     /locus_tag="ACHY3M_002319"
     CDS             12400..13302
                     /gene="rpnE"
                     /locus_tag="ACHY3M_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFS4317228.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IEPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13343..13804)
                     /locus_tag="ACHY3M_002320"
                     /pseudo
     CDS             complement(13343..13804)
                     /locus_tag="ACHY3M_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(13868..15070)
                     /gene="yfaY"
                     /locus_tag="ACHY3M_002321"
     CDS             complement(13868..15070)
                     /gene="yfaY"
                     /locus_tag="ACHY3M_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFS4317229.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(15170..15712)
                     /gene="yfaZ"
                     /locus_tag="ACHY3M_002322"
     CDS             complement(15170..15712)
                     /gene="yfaZ"
                     /locus_tag="ACHY3M_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFS4317230.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            15991..16416
                     /gene="nudI"
                     /locus_tag="ACHY3M_002323"
     CDS             15991..16416
                     /gene="nudI"
                     /locus_tag="ACHY3M_002323"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFS4317231.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16455..17057)
                     /gene="ais"
                     /locus_tag="ACHY3M_002324"
     CDS             complement(16455..17057)
                     /gene="ais"
                     /locus_tag="ACHY3M_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFS4317232.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17365..18504
                     /gene="arnB"
                     /locus_tag="ACHY3M_002325"
     CDS             17365..18504
                     /gene="arnB"
                     /locus_tag="ACHY3M_002325"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFS4317233.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18508..19476
                     /gene="arnC"
                     /locus_tag="ACHY3M_002326"
     CDS             18508..19476
                     /gene="arnC"
                     /locus_tag="ACHY3M_002326"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFS4317234.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19476..21459
                     /gene="arnA"
                     /locus_tag="ACHY3M_002327"
                     /pseudo
     CDS             19476..21459
                     /gene="arnA"
                     /locus_tag="ACHY3M_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21456..22346
                     /gene="arnD"
                     /locus_tag="ACHY3M_002328"
     CDS             21456..22346
                     /gene="arnD"
                     /locus_tag="ACHY3M_002328"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFS4317235.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22346..23998
                     /gene="arnT"
                     /locus_tag="ACHY3M_002329"
     CDS             22346..23998
                     /gene="arnT"
                     /locus_tag="ACHY3M_002329"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFS4317236.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            23995..24330
                     /gene="arnE"
                     /locus_tag="ACHY3M_002330"
     CDS             23995..24330
                     /gene="arnE"
                     /locus_tag="ACHY3M_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFS4317237.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24330..24716
                     /gene="arnF"
                     /locus_tag="ACHY3M_002331"
     CDS             24330..24716
                     /gene="arnF"
                     /locus_tag="ACHY3M_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFS4317238.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24710..24976)
                     /gene="pmrD"
                     /locus_tag="ACHY3M_002332"
     CDS             complement(24710..24976)
                     /gene="pmrD"
                     /locus_tag="ACHY3M_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFS4317239.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25086..26441)
                     /gene="menE"
                     /locus_tag="ACHY3M_002333"
     CDS             complement(25086..26441)
                     /gene="menE"
                     /locus_tag="ACHY3M_002333"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFS4317240.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26438..27400)
                     /gene="menC"
                     /locus_tag="ACHY3M_002334"
     CDS             complement(26438..27400)
                     /gene="menC"
                     /locus_tag="ACHY3M_002334"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFS4317241.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPASTLPVVEVDALERLL"
     gene            complement(27400..28257)
                     /gene="menB"
                     /locus_tag="ACHY3M_002335"
     CDS             complement(27400..28257)
                     /gene="menB"
                     /locus_tag="ACHY3M_002335"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFS4317242.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28272..29030)
                     /gene="menH"
                     /locus_tag="ACHY3M_002336"
     CDS             complement(28272..29030)
                     /gene="menH"
                     /locus_tag="ACHY3M_002336"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFS4317243.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29027..30697)
                     /gene="menD"
                     /locus_tag="ACHY3M_002337"
     CDS             complement(29027..30697)
                     /gene="menD"
                     /locus_tag="ACHY3M_002337"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFS4317244.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30786..32081)
                     /gene="menF"
                     /locus_tag="ACHY3M_002338"
     CDS             complement(30786..32081)
                     /gene="menF"
                     /locus_tag="ACHY3M_002338"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFS4317245.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32160..32465)
                     /gene="elaB"
                     /locus_tag="ACHY3M_002339"
     CDS             complement(32160..32465)
                     /gene="elaB"
                     /locus_tag="ACHY3M_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFS4317246.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32520..32981)
                     /gene="elaA"
                     /locus_tag="ACHY3M_002340"
     CDS             complement(32520..32981)
                     /gene="elaA"
                     /locus_tag="ACHY3M_002340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4317247.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33046..33963
                     /gene="rbn"
                     /locus_tag="ACHY3M_002341"
     CDS             33046..33963
                     /gene="rbn"
                     /locus_tag="ACHY3M_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFS4317248.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34151..>34976
                     /locus_tag="ACHY3M_002342"
     CDS             34151..>34976
                     /locus_tag="ACHY3M_002342"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFS4317249.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFEKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRV"
BASE COUNT         8583 a         9348 c         8645 g         8400 t
ORIGIN      
        1 taatgaataa ttttcttata atataaggta aattaacaaa atggcttcgc atttaacaat
       61 aaccgaatag aaaacaacca tttcgacatc aacaatcgct tacattcgct tatatattga
      121 ccacaactga tacatcagat tatgtgatga ctcgtgctta gatcaatttt tgcaatcatt
      181 agcaaaaaga ttaataaacc atctatatca atttatccaa cctattctgc cgttcaagaa
      241 atcgccgaac agttattttt aacaaatttt tctcttccca ttgactttcc cggacacctt
      301 gtctgaccta aggtgcgcga aagccacttt ttccttcctg agttatccac aaagttatgc
      361 acttgcaaga gggccatttt cacactatct tgcagtgaat cccaaacata ccccctatat
      421 atagtgttct aagcagcttc ccgtactaca ggtagtctgc atgaaactat tgcggaaaga
      481 attccaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa gcgcgacggt
      541 agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc ggcagaagga
      601 ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc acattcagtt ttatgacggt
      661 atcaagacct ctgacatcca cgaaaccatt atcaaggcgg ccgcagacct gatctcccgt
      721 gatgcgccgg attatcagta tctcgccgcg cgcctggcga tcttccacct gcgtaaaaaa
      781 gcctacggcc agtttgagcc gcctgcgctg tacgaccacg tggtgaaaat ggtcgagatg
      841 ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa gcagatggac
      901 acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa gcagctggaa
      961 ggcaaatatc tggtacagaa ccgcgtgacc ggcgaaatct acgaaagcgc ccagttcctt
     1021 tatattctgg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg cctgcaatat
     1081 gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt cgctgccgac gccaatcatg
     1141 tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg tactgatcga gtgcggtgac
     1201 agtctggatt ccatcaacgc tacctccagc gcgattgtga aatacgtttc ccagcgtgcc
     1261 gggatcggca tcaacgccgg gcgtattcgt gcgctgggta gcccgattcg cggtggtgaa
     1321 gcgttccata ccggctgtat tccgttctac aaacatttcc agacagcggt gaaatcctgc
     1381 tctcagggcg gcgtgcgcgg cggtgcggca acgctgttct acccgatgtg gcatctggaa
     1441 gtggaaagcc tgctggtgtt gaaaaacaac cgtggtgtgg aaggcaaccg cgtgcgccat
     1501 atggactacg gggtacagat caacaaactg atgtataccc gcctgctgaa aggtgaagat
     1561 atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg acgcattctt cgccgatcag
     1621 gaagagtttg aacgcctgta taccaaatat gagaaagacg acagcatccg caagcagcgt
     1681 gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac cggtcgtatc
     1741 tatattcaga acgttgacca ctgcaatacc catagcccgt ttgatccggc catcgcgcca
     1801 gtgcgtcagt ctaacctgtg cctggagata gccctgccga ccaaaccgct gaacgacgtc
     1861 aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg ctttcaacct gggcgcaatt
     1921 aatagcctgg atgaactgga agagctggca attctggcgg ttcgtgcact tgacgcgctg
     1981 ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg tcgtcgtacg
     2041 ctgggtattg gtgtgatcaa cttcgcttac tacctggcga agcacggtgt gcgttactcc
     2101 gacggcagcg ccaacaacct gacgcataaa accttcgaag cgattcagta ttacctgctg
     2161 aaagcgtcta acgagctggc aaaagagcaa ggcgcgtgcc cgtggtttaa cgaaaccact
     2221 tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatac catcgctaat
     2281 gagccgctgc attacgactg ggaagcattg cgtgaatcaa tcaaaacgca cggcctgcgt
     2341 aactccacgc tttctgccct gatgccgtca gagacttctt cacagatctc taacgccact
     2401 aacggtattg aaccgccgcg cggttacgtc agcatcaaag cgtcgaaaga cggtattttg
     2461 cgccaggtgg tgccggacta cgagcacctg cacgacgcct atgagctgct gtgggaaatg
     2521 ccgggtaacg atggctacct gcaactggta ggtatcatgc agaaatttat cgatcagtcg
     2581 atctctgcta acaccaacta cgatccgtca cgtttcccgt caggaaaagt gccgatgcag
     2641 cagttgctga aagacctgct caccgcctac aaattcgggg tcaaaacgct gtattatcag
     2701 aacacccgtg acggcgctga agacgcgcag gacgatctgg taccgtcaat ccaggacgat
     2761 ggctgcgaaa gcggcgcatg taagatctga taatccccca gcctttgcac caaaccgtcc
     2821 cctctcccct tgcgggagag ggttagggtg aggggtaata aaaccacagg acacactcat
     2881 ggcatatacc accttttcac agacgaaaaa tgatcagctc aaagaaccga tgttctttgg
     2941 tcagccggtc aacgtggctc gctacgatca gcaaaaatat gacatcttcg aaaagctgat
     3001 cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt gacgtttctc gcgaccgtat
     3061 cgattaccag gcactgcctg aacacgaaaa acacatcttt atcagcaacc tgaaatatca
     3121 gacgctgctg gattccattc agggccgtag cccgaacgtg gcactgctgc cgctgatctc
     3181 gatcccggaa ctggagacct gggtcgaaac ctgggcgttc tcagaaacga ttcattcccg
     3241 ttcctatact catatcattc gtaatatcgt taacgatccg tctgttgtgt ttgacgatat
     3301 cgtcaccaac gagcagatcc agaaacgtgc ggaagggatc tccagctatt acgatgagct
     3361 gatcgaaatg accagctact ggcatctgct gggcgaaggt acccacaccg ttaacggtaa
     3421 aactgtgacc gttagcctgc gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt
     3481 taacgcgctg gaagcgattc gtttctacgt cagctttgct tgttccttcg catttgcaga
     3541 acgcgaattg atggaaggca acgccaaaat tattcgcctg attgcccgcg acgaagccct
     3601 gcacctgacc ggcacccagc atatgctgaa tctgctgcgc agcggcgcgg acgatcctga
     3661 gatggcggaa attgccgaag agtgtaagca ggagtgctat gacctgtttg ttcaggcagc
     3721 tcaacaggag aaagactggg cggattatct gttccgcgac ggttcgatga ttggtctgaa
     3781 taaagacatt ctctgccagt acgttgaata catcaccaat atccgtatgc aggcagtcgg
     3841 tttggatctg ccgttccaga cgcgctccaa cccgatccca tggatcaaca cctggctggt
     3901 gtctgataac gtgcaggttg ctccgcagga agtggaagtc agttcttatc tggtcgggca
     3961 gattgacgcg gaagtggaca ccgacgattt gagtaacttc caactctgat ggccagcgtt
     4021 accctgcgca tcactggcac acaactgctg tgccaggatg aacacccttc ccttctggcg
     4081 gcgctggaat cccacaatgt ggcggttgag tatcagtgtc gcgaaggtta ctgcggctcc
     4141 tgtcgcaccc gtctggttgc aggtcaggtt gactggattg ccgaaccgtt agcctttatt
     4201 cagccggggg aaattttgcc ctgttgttgc cgggcaaaag gcgatattga aatcgagatg
     4261 tgaattggtt gtagtgccag atacaacgct tatgcgtctt atctggccta caacgattac
     4321 atggcgtagt aatacgcttt cacctgctcc cagtccgctt tggggattgg ctccagatat
     4381 ttttccagct ggcggaagtc atgattaatc gctttatcgc gacgcaagcg gcgacgactt
     4441 ttctccaggt caagaaaacc agcttctgca ttaccttctg ttttcacata gatatggcga
     4501 acataacaac agccatgctg acgattaatg ctatgcattt tcttaaacgc cagcgccact
     4561 gctttcaaca tggcttgccg tacttcgtca gaataaggcg atactgcatg ctgggcatac
     4621 cagtcagcaa tgctgatgaa ccccgccata tcttcagtca ccagcaacgc tcgccattca
     4681 ccctcaattt tcaccgcttc accaaaaacg atcttcggca cgatgacacc ggcccgttcc
     4741 agttctttaa ttaccgcaac ctcacggaca atcgttggtc ggccgaacgg ataacgtacg
     4801 gaatgaaaca gatgatgcgt catgcgcttt acatacagct ttttgccgtt gcgctcgacg
     4861 cattgcaccc cgctcatacc attacggcga tagttaggct cttcaaccca gtcgccctct
     4921 gttgcccacc agtgattaaa ttcgtcgtac tttgctgaaa ctgccatacc ctatcgcctg
     4981 tcatttttat taacgacaat gactataggt ggttacctga ggaaaatctt aatgaaacgt
     5041 gtcgtattaa tgaatttctt ttgccactac tagcgtgaca ccgcttttac ccttcatttg
     5101 cagcgcatag attccaccag ccactaccac taatagtgcg cccagtagat aaaatgtgaa
     5161 tttaatcagt cggatacagc gatgaagttt attcgacagg gattaggcat cgccttacaa
     5221 ccagagttaa cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc
     5281 ttctatcgac agatttcgtt gttggctaaa gaaaagccgg tagaaggcag tccactgttt
     5341 ttactacaaa cgtgtacgga acaactcgtg gtgaatggaa aaatttgatg tgagacagga
     5401 tggcgcatga acgccatcct gcagtagcat tactctttat taaggaattt tactgcctta
     5461 tcagggaaat cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac
     5521 tgattcacat cagtagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact
     5581 accagtttat tctgctgagc atcttgcacc atgccagtga gtttgatatt acccggctgc
     5641 gatgtttcct caatcaacat atggtaatcc ggcccgattc cgtcggcata ttccgccacc
     5701 tgtttcatag cacccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc
     5761 ggctgtttct gctgcgtttc attccagtcg gtataggcaa tcaactgtac cagattgaga
     5821 tccatgccca ttttaggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa
     5881 cattgcaaat aaactttgtc gtccttaccg gtgtaaccat atttcttcag cacttccagc
     5941 gtttttgccg caatatcctt cccttcctga tgatggaacc acggcgcttt gatttcagga
     6001 taaataccga tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct
     6061 tcaaaggtgt gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgc
     6121 acttttttac cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc
     6181 agcgtgaaat ctatcgcgta gtaacgaccg tctttgcgcg cccggtccgg gaaacgatcg
     6241 gcaacatcag taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg
     6301 gtcatcacca aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct
     6361 ggcagcgtat gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg
     6421 ttgctgtccg ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg
     6481 ctaaggtttt tcagcgtcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg
     6541 ttcatggcga cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct
     6601 gccgccaatc attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac
     6661 aatcgcgctc gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc
     6721 cgtacctgcc gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg
     6781 accgtagatc aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc
     6841 cgggttcatc cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga aaacgccggt
     6901 tgccccacgg ttgccacgga agactttatc cgacatccag ccgcacagca gagtgcccgg
     6961 aatacctgca tattcataaa ggaagtaggc ccaggaggat ttatccagcg cgaaatgctt
     7021 aacctctttc agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa
     7081 cacgttggcg atggcgatat accacagcag tttgttcggc agcacgtact gcatgaagat
     7141 ttgcttcgcg gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt
     7201 gtactcttcg atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc
     7261 gaataatgcc accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt
     7321 gaaccaggcc atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc
     7381 acagttccac actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt
     7441 acgaccacac ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa
     7501 catcaccgca atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc
     7561 cgccagaatc aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc
     7621 cattatgaat ttcgaaaatc cataagcgat cgagatcccc gaaagcgcga aacctaaatc
     7681 accgcgagag aatccctgct cgaccagata aggcatagca agcgcaaagt tcttacgaac
     7741 caaatagtaa gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg
     7801 ataagtcgga tcgatctccg cagcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat
     7861 actcaacatt gaaagcctcc gtggcccgtg gtcttattta tgattaacag actgattcag
     7921 tgggagaacc tgccgtttct ttagttgccg cgatgttaag aaaacattca gaaattaaat
     7981 gtgaattgcc gcacacatta ttaaataaga tttacaaaat gttcagaatg acgcatgaaa
     8041 tcacgtttca ctttcgaatt atgagcgaat atgcgcgaaa tcaaacaatt catgttttta
     8101 ctatggctaa atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc
     8161 aatcaagtga cgtgattatc attggtggcg gcgcaacggg agccgggatt gcccgcgact
     8221 gtgccctgcg cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca
     8281 ccgggcgtaa ccacggcctg ctgcacagcg gtgcgcgcta tgcggtaacc gatgcggaat
     8341 cggcccgcga atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg
     8401 aaccaaccaa cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt
     8461 ttattcgcgc ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc
     8521 gcattatcga acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca
     8581 ccgttgatcc atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg
     8641 ttatccttac cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg
     8701 ttcgtgtacg taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta
     8761 atgccgctgg gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt
     8821 tcccggcgaa aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc
     8881 gctgccgtaa accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg
     8941 gcaccacctc tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag
     9001 aggttgatat tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca
     9061 ttttgcgggc ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac
     9121 gtaacgtcag ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat
     9181 ttatcaccat caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg
     9241 acgcggtatg ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc
     9301 ctggttcaca agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc
     9361 tgcgcggttc tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc
     9421 gtctgcatcg tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gttcagtacg
     9481 cggtagaaaa tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga
     9541 tgggtacctg ccagggcgaa ctctgcgcct gccgcgctgc cggactgctg caacgtttta
     9601 acgtcacgac gtctgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga
     9661 aaggcgtgca acccatcgcc tggggagatg cactgcgcga aagcgaattt acccgctggg
     9721 tttatcaggg attgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt
     9781 atgggcggcg gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc
     9841 tgtgccattg tcacgcgtgg tcaaagcgcg ctgcatttct catccggttc gctggatttg
     9901 ctgagccatc tgcctgatgg tcaaccggtg acagacattc acagtggact ggaatctttg
     9961 cgtcagcagg caccagccca tccttacagt cttctcgaac cacaacgcgt gctcgatctc
    10021 gcttgccagg cgcaggcatt gatcgctgaa agcggtgcgc aattgcaggg cagcgtggaa
    10081 ctggcccatc agcgcgttac cccgctcggc actttgcgtg caacctggtt aagttcgcca
    10141 gaagttcccg tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg
    10201 atggatttcc aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa
    10261 accgcagaaa tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc
    10321 gcggtgaata tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg
    10381 cttattcctg tcgctaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc
    10441 gatgacaaac tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca
    10501 acgctgccgc cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg
    10561 cgccagggtg gcgtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc
    10621 gtagtgaatg aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg
    10681 gttctcgcca gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc
    10741 gagccgattc tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc
    10801 gatttttttg cgccgcaacc gtggcagcag ttcggtgtaa ccactgatca gacgctacgc
    10861 ccgtcgcagg cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga
    10921 tttgatccca tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc
    10981 gctcaacaga ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact
    11041 gcattaagtg caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc
    11101 cagggccaaa acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt
    11161 atgacgaggc gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg
    11221 atgtgaagat tggcgatatt atccagcgcg cgcgggcaaa atatgacacc acgcgcccgt
    11281 cgctgcgtaa ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg
    11341 caccaatcgt caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt
    11401 taaaaatcga tcatcgccgc acgctaccga aatactcctt cggtacgttt cgtcgctggt
    11461 atcgcagcat agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct
    11521 gcttcgttaa ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa
    11581 tgggtaccgg tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca
    11641 acggctttac cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag
    11701 ctgtgggagt aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc
    11761 gcgacgaata cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac
    11821 tggcaacccg ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc
    11881 tgccgctgaa agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct
    11941 acaccctgga gctgttgcgt aaaatcccgg ggcttgagtt aacggtgctg gattcccagt
    12001 gctgcggtat tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca
    12061 tcggtgcgcc actgttccgc cagatagaag agagcggcgc ggatctggtc atcaccgact
    12121 gcgaaacctg caaatggcag attgagatgt ccacgagcct tcgctgcgaa catccgatta
    12181 cgctactggc ccaggcgctg gcttaaccga ccttttgatg cccggtaagc acattgctta
    12241 tcgggcattt ttgcgagcgc gattccacgc accatgtatg cgttgcaacg ctttgaaaat
    12301 tcctctgaaa atgtctcgca aaggctgaaa ctgacagatg tcaaaggccc gggatagacg
    12361 taatgtcgcg tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacatcc
    12421 tccccgcatg atgcggtgtt taaaaccttt atgttcacac ccgaaaccgc acgggatttt
    12481 ctcgaaatac atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg
    12541 gaacccacta gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc
    12601 gtggaaacca gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca
    12661 gaaaagaata tggcttttcg gctaatgcgc tatgccactg ccgccatgca gcgtcacctg
    12721 gataaaggct atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca
    12781 tcgccctacc cgtactcact taactggctg gatgagtttg acgatccgca acttgcccgg
    12841 cagttgtaca ccgaagcttt tccgttggtg gatatcacca tcgaacctga cgatgagatc
    12901 atgcaacatc ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccgcgat
    12961 ttaatcggca tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc
    13021 cagctacaaa cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt
    13081 tttattgagg agattgccga acgttcacca ctacaaaagg agagattaat gactattgct
    13141 gaacggctac ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc
    13201 attaaaattg ctttgcgcat gctggaacag ggcattgatc gtgaccaggt gctcgcggcc
    13261 acccagctaa gcgaagccga tctggcagcg aataaccact aattaacaca ggcccacagc
    13321 cgatccccat gggcctttga tatcaataac taccttttat gcgtggccca tttttgcctg
    13381 atttaaacat cgccagtcgt tgatccaggg catcgctgta gagcatcgtg tcaacgccaa
    13441 cagcgacaaa gttcgctccc cacgccaggc attgctgcgc catatcagga gccacagcca
    13501 gaaaaccagc cgctttaccc gcagcacgga tccgccgaat gctggtttca ataattcgct
    13561 gcacttccgg gtgcccggcg ttatccgggt agcccaacga cgcagaaaga tccgctggtc
    13621 caataaacac gccatcaatc ccttcgacgt cgaggatctc gtccaggtta tccagtgccg
    13681 ttttactttc cacctgcact aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct
    13741 caatgcgtcc ccagcgcgca gcccgtgcca cactggcccc aacaccacgc tcaccgtagg
    13801 gagggttgga caagttttgc cggaggggga atgtgccggt gcagtaaacc ggcacagagt
    13861 ggcggaatca gacagataag gtcatggact caataacctc aatccagcca tgctcactgg
    13921 cgatatcctg gccgtttaac caacggcgca gcatattcag tgccatcatt gcgcacactt
    13981 cctgacggat agccaggctg tagcgcgtag tgctgaaacg cacacgcaga gcgaaagtgc
    14041 cgtctggtgt ggctagcgca aagttgagat gttcgttctc gaaacccgaa acagccagtg
    14101 ccagcccggc aaaatggttg gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg
    14161 tttcttcctg tgaaggaacc acttcacacg ccagcaatgg agcacctgcg cgagaaagtt
    14221 gcaaagccaa taaaccaccg gtgaattgct cgctcaacgt caggctgaac tggcggtttt
    14281 gcaattcgcg actgatctgc gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc
    14341 cggcaacacg tttaacgtcc agccacagtt tttccatcgc ctgttgctcg cttgccggtc
    14401 cggtgagttt cagttcgata atcggcattg aagagcgata gcccattgtt acgcccggcg
    14461 gcagttgtag agtgtccagg ctttgtgcca gatcgctttc cgaacgacca aaggtagtta
    14521 aacgcagaca aaccggcggc tgtggtaaag aaaaacgctc gcgcagacgc ggcaggattt
    14581 cgtgctcgac catcacctta aattctgacg gtacgccggg agtaaagaac atcaggcaac
    14641 gattaagctg cacggcaaaa ccacaggcgg tgcctaccgg gttattgata aattcagcac
    14701 tggcaggcag ctccgcttgt ttacggttgc tcggtgccat tactcgtcca cgttcgtgga
    14761 aatagcgttc catctctttg agccaggctt catgcagcac catgccttca ccttttgctg
    14821 tcgcagcggc gagtgcgctt aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca
    14881 gcacatcggc gtgctgacta cgttcgcgaa gaatggtgac taagtcatca aggttatccc
    14941 ccaccgtatt gcggcgagat aatggcagcc cctgatgaaa gaaaaaatcg gccagccagg
    15001 cagcgttagt gtcaacgatt tgcccgtgta acacttcatc cccggtggat agcatttcca
    15061 cttttaacat ggttatctcc tgcttttgtt atcgaatcac tataacgcaa agggagataa
    15121 ggcgtagtaa gaaactgaat ggcgcgacag aatgccgcgc cggaagggat cagaaactgg
    15181 cgttaacccc aacatacggg ccgtcagcca cggcgttgtc gcggttaccg tctttacccg
    15241 acagattcag gtagcgataa cccgcctcaa tactgactgg acgcataatg gtgtaacgcg
    15301 cgccagcatt cgcttcctca taactttgaa taccgctgga gagcgaatcc ggagagtagt
    15361 aatactcgcc aaacaaacgg aagctgttgc caattttcca ctgcaaacca cctcctaccg
    15421 ctgcggcata accttcatcg ccgtaattcg ggttggtgta aacgcctttt ccgccaacgg
    15481 tcgccattaa cggcccgaga ggcagattca accccagccc cacgcccgcg acgtcaccgt
    15541 cgtcgtcgtt atgtgtccag ttaccgctta aagccaggcc cgtcgattca gtcccaaaac
    15601 cgacaccaat attggtgtat tctttacccg cctggccgct gatgctcatt gcattgaccg
    15661 atgcagaaac caacagcatt ccggccagac ctgctagcgc aatttttttc attatcccct
    15721 ttcccacaag caattaatca aaattcccaa agctgcggga ttgtactgcc tggcacagag
    15781 gattcaatga gcgaaaaaag ggatctagtc acttattgta actaaatact tcttaaataa
    15841 ttaaacgccg aaaaaataaa cacactttac cgatacacac aaaaataata ataagtgcag
    15901 ttatcattgc aaaagagatt actgtcactt tcacatattt ctgagttacc gtattctcat
    15961 ggtttctttt tatttataag gaatacccct atgcgacaaa ggactattgt atgccctttg
    16021 attcaaaatg atggtgctta tttgctgtgt aaaatggccg acgatcgcgg cgttttcccc
    16081 ggtcaatggg cgctttcggg tggcggcgtg gagtctggcg aacgaattga agaggcacta
    16141 cgccgcgaaa ttcgcgaaga actgggagaa cagctgcttt tgacagaaat cacgccgtgg
    16201 accttcagcg atgatattcg caccaagacg tatgcagatg gtcgcaagga agagatttat
    16261 atgatttacc tgatttttga ctgcgtttct gccaaccgag aagtgaaaat aaacgaagag
    16321 tttcaggact acgcgtgggt aaaacctgaa gatctggtgc attatgattt aaatgttgcc
    16381 acccgaaaaa cgttacgttt gaaaggtctt ctttaacaat gatgacagcc atcactggct
    16441 gtcagattga aaatttagtg gttaacgaat tccccatcca gataaacttt gcctttttcc
    16501 acatgcatga ctaaaccatc cagataatca ggtttaaatg tcgcatcacg cttattttta
    16561 gcaatatatg tcaggcaatg attatgggtg aaaataacga tatttttatc aggcgctttg
    16621 ctttgtaagt ccttaattgc actataaatc tcattaccgc actgaagaag tcgtttatct
    16681 accgtcagct ttttacccgc ggaaaaccag gtcgccgact gaatggtccg gacggtatta
    16741 ctggaataaa gatcgaaatc agggatgtca gcgctaaaag cgttgcccag ttcacgggca
    16801 tcctgggtac ctttaaccgt aatacctgtt ttatctgaca agcattgatt ggttgaacga
    16861 tcgcaacgtt cagcatggcg aaacaaaacg acaaccgggt gctgctgtgc cagtctggcc
    16921 aatgttttat tgtcgatacg tggcaaacca ttgctactcc aggcggcatg agtacccagt
    16981 ccggcaattg cagcgagcgc cagtaaaatg atgaaatatt tttttgactt caatgatgag
    17041 cggcaaaaag ctaacacgta aactccacct atagacaagc gcaaccagac aattaccgta
    17101 aaattgagcg acatttctgg cgataattag caattggtgt aatattaaaa atcctacgat
    17161 gtcggcaaaa tgcctcaaaa ttttgccaaa tgcaaagcct aaataagaaa aaatataaaa
    17221 atttcaatat ttacgtctaa tattagtttc ttaaggttaa gttaatattc tatccttaaa
    17281 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
    17341 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
    17401 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
    17461 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
    17521 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
    17581 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
    17641 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
    17701 gcgatcgagt cagccataac gccacgcact aaagccatca ttccggtgca ttatgccggt
    17761 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
    17821 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
    17881 accactattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
    17941 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
    18001 gtcgatgcct atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg
    18061 ggctataagt acaatctgac cgatattaac gccgcaattg ccctgacaca gttagcaaaa
    18121 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
    18181 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
    18241 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
    18301 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
    18361 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
    18421 tgttctttgc cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc
    18481 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
    18541 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
    18601 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
    18661 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
    18721 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
    18781 accggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
    18841 cgcctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
    18901 caggacagct ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc
    18961 accggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
    19021 gatgcgatgt tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc
    19081 gcccgccgtg ccattgaaat tccagtacat cattccgagc gtgagtttgg tgaatccaaa
    19141 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
    19201 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
    19261 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
    19321 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
    19381 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
    19441 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
    19501 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
    19561 tttttaccca taccgataat cccggtgaaa aagcctttta tggttcggtg gctcgtctgg
    19621 cagcggaaag aggcattccg gtttatgcgc cggatgacgt taatcatccg ctgtgggtgg
    19681 aacgcattgc ccaactgtcg ccagatgtga ttttctcttt ttattatcgc catcttattt
    19741 gcgacgaaat tttgcagctc gctcccgcag gtgcatttaa tctgcatggt tcgctgttac
    19801 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt caacggtgaa acggaaactg
    19861 gcgttacatt gcaccgaatg gtgaaacgtg ccgatgccgg ggccattgtg gcgcaactgc
    19921 gcattgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
    19981 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
    20041 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg
    20101 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
    20161 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
    20221 ctcatgccag caaagcacaa gccggggagc gtgatttctg ttgcgccact gctgattgcc
    20281 tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag
    20341 ggctcgcaat tagcgcagac gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct
    20401 gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc
    20461 aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt
    20521 ggcagtgatg cgataagccg ttttctgaat catccgcatt ttcactttgt tgaaggcgat
    20581 atcagtattc attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg
    20641 ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc
    20701 gattttgaag agaatctgcg cattatccgc tactgcgtga agtaccgtaa gcgaatcatc
    20761 ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat
    20821 tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt atcaaaacaa
    20881 ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc
    20941 cgcccgttta actggatggg accgcgactg gataacctta atgcggcacg aatcggcagc
    21001 tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccgat taagctgatt
    21061 gatggcggaa aacaaaaacg ctgctttact gatattcgcg atggtatcga ggcgttatac
    21121 cgcattatcg aaaatgcggg aaatcgctgc gacggtgaaa ttatcaacat tggcaatcct
    21181 gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat
    21241 ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagcagctac
    21301 tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc
    21361 tgcctggact gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc
    21421 ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg
    21481 atgtcgatac ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta
    21541 agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc
    21601 tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaat gcggcatcgc
    21661 tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg
    21721 ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggcctg cacgcctggg
    21781 atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata
    21841 ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg
    21901 ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct
    21961 acaacagcga ttgtcgtggg gccatgccgt tccgtccatt gctcgaatca ggaaaccctg
    22021 gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga
    22081 aagcagaaga ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg
    22141 tttataccat tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc
    22201 tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga
    22261 cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt
    22321 gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcctcttcgc
    22381 gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga
    22441 aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca
    22501 tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg
    22561 gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct
    22621 gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc
    22681 tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc
    22741 cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc
    22801 aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg
    22861 cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct
    22921 gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt
    22981 tatcagttgc gtactgacgg ttctcccttg gggactggcg atagcgcagc gggagcctga
    23041 cttctggcac tattttttct gggttgagca tattcaacgc tttgcactgg atgatgccca
    23101 acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct
    23161 gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt
    23221 ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta aaggtaaatt
    23281 gcccacctat attctttcct gctttgcatc tctggcaatg ctgatggcgc attacgcttt
    23341 gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt
    23401 tggcgtcact ggcattattg ccacatttgt ggtctccccg tggggaccaa tgaacacgcc
    23461 ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg
    23521 ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggcctt ttgccgcgct
    23581 ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag ttatggaagg
    23641 aaaacatccg caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat
    23701 tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat
    23761 catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg
    23821 gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat
    23881 tattactctc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc
    23941 cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc
    24001 tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag
    24061 gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg
    24121 gcgctggctt gtcttggtct tgccatggtg ctctggctgc tggtcttgca gaacgtaccg
    24181 gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta
    24241 aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt
    24301 ggcattgtga tcctcgggag tacggtgtaa tgggcctgat atggggatta ttcagcgtca
    24361 ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga
    24421 cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt
    24481 tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac
    24541 ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt
    24601 caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct
    24661 gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt
    24721 tccctgccgc tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga
    24781 ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga
    24841 gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc
    24901 cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt
    24961 tgaccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat
    25021 taacagataa actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg
    25081 gttaattatt gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg
    25141 ccgtttttca gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc
    25201 ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc
    25261 ggccgatgac caaactcctt gtcggcaacg ggaacgataa acacctgcaa aaccgcagga
    25321 tgtgcagcaa taacacgctc gacttcttcc ggctgaatac cctctccgcc actgaagaat
    25381 agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc
    25441 gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct
    25501 tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc
    25561 ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt tcgcacacac cgtggaggca
    25621 aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc
    25681 aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg
    25741 ttaaccagca aacgccagag ttgtgttggc accagtgaag catgagtaca gcctgccagc
    25801 atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc
    25861 cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga
    25921 tcgccaaacg gaatcaacga taacacccct tcggcactgg caagatgggc ctgataagta
    25981 tggacagcgg ctttcggcaa cccggtagaa cccgaggtca acgtcattga gcacagacgc
    26041 gtcggctgcc acgtagcggc atgtgcgcct tcaacccgct gaatgtgcag cgacgttaac
    26101 gccggaaacg tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc
    26161 aattcttcaa gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat
    26221 tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg
    26281 ccgctgccct caacaacgcc ctgcgccgca aagcctgagg ctaattcatc gacgcgagca
    26341 caaagctcgc gccagttaag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc
    26401 acttgccgcc agtgacgcca cggccagtca gagaagatca taacaaccgc tccagtgcat
    26461 caacttccac gacaggcagc gtgctagccg gccagcgacg tacctgctgc gcctgcatca
    26521 gatccagcgt gtccagccct ggaatggtgt ccggcgttaa ccaggcggca atccgcgcca
    26581 gttgcgttaa gcctaagctc gattcaatgg aagaactgat caccgccgtc agccccagcg
    26641 cgtgcgccgc ctgtacctgc tcgcgtactt tctccagact gcccgtgagc gtgggtttga
    26701 taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaagcttt
    26761 catcccaggc aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt
    26821 tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa
    26881 actgctggcc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg
    26941 gaattgcctc cagcaacaga ttaaccacca tgccgtcgcg cacggcttcg tacaatccca
    27001 ctttgacctt cgccactttc tcgcctggca tatcagcaag tttgaggatc agatcgtcag
    27061 gatcgccatt acacagtggt gccgcacggt aattggccgc ctgcgggagc gtatctgcca
    27121 gctccgccaa tgcacagctt acgccaaagg caacggaagg catctgcggt agctcgcaat
    27181 cgcctgccag ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccaggttt
    27241 cctggctgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca
    27301 ggcaaacgta cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt
    27361 ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt
    27421 gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt
    27481 catgtagaac agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca
    27541 gtcggcgttc agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc
    27601 gcggcaccaa cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag
    27661 gcccatatcc agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc
    27721 ttttttctgc ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc
    27781 gactttcggg ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat
    27841 catgtgcaga acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt
    27901 cggacaggta cggatctgac gctggaagtc cagcacgttc aagtgatgta cgccggaatc
    27961 atctttatag ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt
    28021 atcgcctgcg ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag
    28081 tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt
    28141 aatggtgatt tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc
    28201 ggagcagtcg tgccattcaa ccggtgcgta aagcattgct tcatcaggat aaatcatatt
    28261 gtgtccttta gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt
    28321 tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc
    28381 gccagggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg
    28441 gcgctaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg
    28501 gcaagggttg cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca
    28561 ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct
    28621 gtgcaaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca
    28681 ttttgcagcc ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg
    28741 caagccgcca tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg
    28801 atgttgtaac taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg
    28861 ctaatcgccg ccgaaccacc atgacctggg agatcaacat acaaccgtga gtagtcggca
    28921 aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc
    28981 agccagggta aacctggttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta
    29041 cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa
    29101 tcaccgtggt ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt
    29161 tttgcggacg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt
    29221 gcggcatcag atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt
    29281 gcccgccgtt gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg
    29341 ccagcgcgtt gagatcgtaa agtgcggaga gatcgcccac aatcgccagc gtcggtttgc
    29401 cgcttgcccg ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc
    29461 ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca
    29521 ccaggctgtt accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg
    29581 ccagttgcgc ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg
    29641 ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc
    29701 agtcggcaat attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt
    29761 caatgtcatc aacaatccag tactcttcgg gttcacagct tgcctgccat tgcaggagcc
    29821 gtttccccgt caggctgctt cccagttgca ccacaatttg cgcctgctgc agctcgctgg
    29881 tcgctttggc attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca
    29941 gcacatcgcc aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct
    30001 cttccgcact catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt
    30061 cgcgctgttt ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc
    30121 accagtcacc cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg
    30181 gttcagcaaa cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt
    30241 cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac
    30301 tgtgcgtggg gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt
    30361 caattagctc cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg
    30421 cttcaatcag tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca
    30481 ccggctgctt gctgactttc gccagcccca gcgccagatg ccccagtcca cgctcatcga
    30541 aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac
    30601 gcgagcctgg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga
    30661 ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg
    30721 cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact
    30781 cattactatt ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc
    30841 cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatatag ccgcacgaca
    30901 ttgccgctga tttttgctga acgcagggaa acgcagaatt cgctttgttg taatgagaga
    30961 tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt
    31021 cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg atgtaaacag
    31081 atcacatcat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta
    31141 cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt
    31201 tggcagatat cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc
    31261 cactctccta actgctgcgc ctgcttatca tcaggattat ttgctactgt tcccgccagc
    31321 gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga
    31381 aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga
    31441 ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga
    31501 gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat
    31561 tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca
    31621 ggcaagggct tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc
    31681 tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc
    31741 cattccaggc ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa
    31801 atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac
    31861 gtaaaacggg taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag
    31921 tagaattgcg ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg
    31981 agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa
    32041 tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa
    32101 aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt
    32161 taacggcgtg ccagcaacag tcctagtacc agcccaacgg ccgcgcccac accaattcct
    32221 tgccagggtt tttcgtggac gtagtcatca gcacgataaa ctgcctgctt cgcccgatag
    32281 taataactgt ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca
    32341 cgcgctttca gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct
    32401 tccagtgttt cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta
    32461 gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag ggttgttgat
    32521 tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc
    32581 tccgtcacag ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg
    32641 aggtaaacag gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc
    32701 tgctgcccca ctttttcgcc acgcaacgct tcactgacaa tcacccgacc tataacgacc
    32761 ggctcaagat catcatcact tttcagaatc ctcgcatatg ccaccagttc atcatttttc
    32821 caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg
    32881 caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa
    32941 accgataatt cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg
    33001 tatgccttat actagccgga atattttttg aaacgtgatg aactcatgga attaattttt
    33061 ttaggtactt cggccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac
    33121 ctgcaacatc cgacccagag cggtctttgg ttgtttgact gcggtgaagg cacccagcat
    33181 cagctactgc ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt
    33241 catggcgatc atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt
    33301 atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg
    33361 cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa
    33421 attctcgatg atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt
    33481 tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa
    33541 gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg
    33601 ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa
    33661 gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt
    33721 gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt
    33781 cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggtaag
    33841 ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcagca cctgttacgt
    33901 gaatgcaggt caattttccc ggcgactgaa ctagcgaatg atttcaccgt gtttaacgtt
    33961 taactctgct tattattaaa aacagggcga aacttgccct gttatcgcaa cccgcgctac
    34021 atatatccac ccacacgggc atattgagcg atttttgata tttattcatc tccttacgca
    34081 ctatattcag agcaataagt tgcctctttt tatcttcatt cagcacaacc tttgcaacta
    34141 aaaggagccc atgatggtta cagttgtcag caattattgt caattatctc aaacccaact
    34201 cagtcagaca tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa
    34261 aacagcgtta tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta
    34321 tgatgaattt gaaaaaaaag ctgaagatat tctttaccat attgttagaa acccaacaaa
    34381 tgagacccta tcgattatcc gacttattaa aaatgcctgt ttaaaattat ataatttggc
    34441 acataccgca accaactccc accttaaacc aactggtccg gataattcag atgttctcct
    34501 gttcaaaaag ctattctccc cttcgaaatt aatgacaatt atcggtgatg aaattcctct
    34561 tatatctgaa aaacagtcgc tttcaaaggt gcttttaaat gatgagaata atgaactgag
    34621 tgatgataca aacttctggg ataaaaatcg tcaattaacc acagatgaaa tagcttgcta
    34681 tcttcagaag atcgccgcta atgcaaaaaa tactcaagtc aattatccta ctggtctcta
    34741 cgtcccctac tccaccagaa ctcacctgga agacgctctc aatgaaaata ttaagagcga
    34801 tccatcatgg ccgaatgaag tccagttatt ccacataaat actggcggac actggatatt
    34861 agtttcgcta cagaaaatag taaataaaaa aaataataaa ctacaaataa aatgcgtcat
    34921 attcaactca ttgcgtgcac taggccatga taaagaaaat tcacttaagc gtgtca
//
LOCUS       DAZUFB010000043        34780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZUFB010000043 DAZUFB010000000
VERSION     DAZUFB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.043"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..732
                     /gene="elfD"
                     /locus_tag="ACHY3M_002343"
     CDS             31..732
                     /gene="elfD"
                     /locus_tag="ACHY3M_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4317250.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            757..3357
                     /gene="elfC"
                     /locus_tag="ACHY3M_002344"
     CDS             757..3357
                     /gene="elfC"
                     /locus_tag="ACHY3M_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS4317251.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3348..4418
                     /gene="elfG"
                     /locus_tag="ACHY3M_002345"
     CDS             3348..4418
                     /gene="elfG"
                     /locus_tag="ACHY3M_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317252.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4430..4972
                     /gene="ycbU"
                     /locus_tag="ACHY3M_002346"
     CDS             4430..4972
                     /gene="ycbU"
                     /locus_tag="ACHY3M_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317253.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4980..5495
                     /gene="ycbV"
                     /locus_tag="ACHY3M_002347"
     CDS             4980..5495
                     /gene="ycbV"
                     /locus_tag="ACHY3M_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317254.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5461..6198
                     /gene="ycbF"
                     /locus_tag="ACHY3M_002348"
     CDS             5461..6198
                     /gene="ycbF"
                     /locus_tag="ACHY3M_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS4317255.1"
                     /translation="MPARHLYFIMTNTWNRLTLLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6309..7319
                     /gene="pyrD"
                     /locus_tag="ACHY3M_002349"
     CDS             6309..7319
                     /gene="pyrD"
                     /locus_tag="ACHY3M_002349"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFS4317256.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7493..8035
                     /gene="zapC"
                     /locus_tag="ACHY3M_002350"
     CDS             7493..8035
                     /gene="zapC"
                     /locus_tag="ACHY3M_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFS4317257.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8032..9141)
                     /gene="ycbX"
                     /locus_tag="ACHY3M_002351"
     CDS             complement(8032..9141)
                     /gene="ycbX"
                     /locus_tag="ACHY3M_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFS4317258.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9385..11493
                     /gene="rlmKL"
                     /locus_tag="ACHY3M_002352"
     CDS             9385..11493
                     /gene="rlmKL"
                     /locus_tag="ACHY3M_002352"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFS4317259.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11505..13412
                     /gene="uup"
                     /locus_tag="ACHY3M_002353"
     CDS             11505..13412
                     /gene="uup"
                     /locus_tag="ACHY3M_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4317260.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13542..14795
                     /gene="pqiA"
                     /locus_tag="ACHY3M_002354"
     CDS             13542..14795
                     /gene="pqiA"
                     /locus_tag="ACHY3M_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFS4317261.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14800..16440
                     /gene="pqiB"
                     /locus_tag="ACHY3M_002355"
     CDS             14800..16440
                     /gene="pqiB"
                     /locus_tag="ACHY3M_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFS4317262.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16437..17000
                     /gene="pqiC"
                     /locus_tag="ACHY3M_002356"
     CDS             16437..17000
                     /gene="pqiC"
                     /locus_tag="ACHY3M_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFS4317263.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17256..17423
                     /gene="rmf"
                     /locus_tag="ACHY3M_002357"
     CDS             17256..17423
                     /gene="rmf"
                     /locus_tag="ACHY3M_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFS4317264.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17493..18011)
                     /gene="fabA"
                     /locus_tag="ACHY3M_002358"
     CDS             complement(17493..18011)
                     /gene="fabA"
                     /locus_tag="ACHY3M_002358"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFS4317265.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18080..19840)
                     /gene="ycbZ"
                     /locus_tag="ACHY3M_002359"
     CDS             complement(18080..19840)
                     /gene="ycbZ"
                     /locus_tag="ACHY3M_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4317266.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20026..20478
                     /gene="matP"
                     /locus_tag="ACHY3M_002360"
     CDS             20026..20478
                     /gene="matP"
                     /locus_tag="ACHY3M_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFS4317267.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20554..21594)
                     /gene="ompA"
                     /locus_tag="ACHY3M_002361"
     CDS             complement(20554..21594)
                     /gene="ompA"
                     /locus_tag="ACHY3M_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFS4317268.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21951..22460)
                     /gene="sulA"
                     /locus_tag="ACHY3M_002362"
     CDS             complement(21951..22460)
                     /gene="sulA"
                     /locus_tag="ACHY3M_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFS4317269.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22724..23308
                     /gene="sxy"
                     /locus_tag="ACHY3M_002363"
     CDS             22724..23308
                     /gene="sxy"
                     /locus_tag="ACHY3M_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFS4317270.1"
                     /translation="MATLGTIEYRSLFGSYSLTVDDTVFAMVSDGELYLRACEQSAQY
                     CVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSLDAALKEKSTRNTRE
                     RLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNSLVTEKILFMLEGAI
                     IGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23271..25433)
                     /gene="yccS"
                     /locus_tag="ACHY3M_002364"
     CDS             complement(23271..25433)
                     /gene="yccS"
                     /locus_tag="ACHY3M_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFS4317271.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25443..25889)
                     /gene="yccF"
                     /locus_tag="ACHY3M_002365"
     CDS             complement(25443..25889)
                     /gene="yccF"
                     /locus_tag="ACHY3M_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4317272.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26012..28066
                     /gene="helD"
                     /locus_tag="ACHY3M_002366"
     CDS             26012..28066
                     /gene="helD"
                     /locus_tag="ACHY3M_002366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFS4317273.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28098..28556)
                     /gene="mgsA"
                     /locus_tag="ACHY3M_002367"
     CDS             complement(28098..28556)
                     /gene="mgsA"
                     /locus_tag="ACHY3M_002367"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4317274.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28652..29314)
                     /gene="yccT"
                     /locus_tag="ACHY3M_002368"
     CDS             complement(28652..29314)
                     /gene="yccT"
                     /locus_tag="ACHY3M_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFS4317275.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29487..29900
                     /gene="yccU"
                     /locus_tag="ACHY3M_002369"
     CDS             29487..29900
                     /gene="yccU"
                     /locus_tag="ACHY3M_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4317276.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29945..30262)
                     /gene="hspQ"
                     /locus_tag="ACHY3M_002370"
     CDS             complement(29945..30262)
                     /gene="hspQ"
                     /locus_tag="ACHY3M_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFS4317277.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30320..31510)
                     /gene="rlmI"
                     /locus_tag="ACHY3M_002371"
     CDS             complement(30320..31510)
                     /gene="rlmI"
                     /locus_tag="ACHY3M_002371"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFS4317278.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31605..31883
                     /gene="yccX"
                     /locus_tag="ACHY3M_002372"
     CDS             31605..31883
                     /gene="yccX"
                     /locus_tag="ACHY3M_002372"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4317279.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31880..32209)
                     /gene="tusE"
                     /locus_tag="ACHY3M_002373"
     CDS             complement(31880..32209)
                     /gene="tusE"
                     /locus_tag="ACHY3M_002373"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFS4317280.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32300..32959)
                     /gene="yccA"
                     /locus_tag="ACHY3M_002374"
     CDS             complement(32300..32959)
                     /gene="yccA"
                     /locus_tag="ACHY3M_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFS4317281.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33367..34382)
                     /locus_tag="ACHY3M_002375"
                     /pseudo
     CDS             complement(33367..34382)
                     /locus_tag="ACHY3M_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(34360..34602)
                     /locus_tag="ACHY3M_002376"
     CDS             complement(34360..34602)
                     /locus_tag="ACHY3M_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS4317282.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(34670..>34780)
                     /locus_tag="ACHY3M_002377"
     CDS             complement(34670..>34780)
                     /locus_tag="ACHY3M_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS4317283.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
BASE COUNT         8920 a         8306 c         9031 g         8523 t
ORIGIN      
        1 gatattcata ttttttaaga gtgactattt atgaaaactt gcataacaaa gggaattgtg
       61 accgtaagtt taacggcaat attactcagc tgctcatccg catgggcggc aggtaaaggc
      121 gggattggac ttgcagcaac acgtcttgtt tattcagaag gtgaagagca aatttcactg
      181 ggtgtacgta acaccagtcc ggatgttcct tatcttattc agtcatgggt gatgacccca
      241 gataataaaa aatcagcaga ttttattatt acaccaccgc tatttgtgct gaatccggct
      301 aatgagaatc tgttacgcat tatgtacatt ggtgcgccgt tggcgaaaga cagagaaacc
      361 cttttcttca ctagcgtacg ggcagtccct tcaacaacga agcgggaaga gggaaatacc
      421 ctgaagattg ccacacaaag cgtcatcaaa cttttctggc gaccaaaagg tttagcgtat
      481 cccttaggcg aggctccggc gaaactgcgt tgcacttcgt cagctgacat ggttacggtc
      541 agtaacccaa caccttattt cattaccctg acagacctga aaataggtgg aaaagtagtt
      601 aaaaatcaaa tgatttcccc ctttgataaa taccaatttt ctctgccaaa gggggccaaa
      661 aatagcagcg taacgtatcg aaccatcaat gactacgggg cggaaacgcc gcaactcaac
      721 tgtaaatcgt aagccgtctt cagttaagag agcgagatgt atagaactca tcgacaacac
      781 agcctgttaa gctctggtgg agtgccatcg tttattggtg ggctggtggt gtttgtgtcg
      841 gcagcgttca atgcacaagc tgaaacctgg ttcgatcctg cttttttcaa agatgatccc
      901 tcaatggtgg ccgatttgtc tcgtttcgaa aaaggacaaa aaataacgcc aggggtttat
      961 cgtgtcgata ttgttctgaa tcagacaatt gtagatacgc gcaacgtcaa ttttgttgag
     1021 ataacgccag agaaggggat tgccgcctgt ttgacgactg aaagcctgga tgcaatgggt
     1081 gttaatactg atgcgtttcc ggcttttaaa caactggaca aacaagcgtg tgtgccattg
     1141 gcggagatta ttccggatgc cagcgtaacg tttaatgtga ataaactccg tctggaaatt
     1201 tcagtaccgc aaatcgccat caaaagtaat gcccgtggtt atgtcccccc tgaacgttgg
     1261 gatgaaggga tcaacgcgct attactggga tattcgttta gcggggctaa cagtattcat
     1321 agcagcgcag acagtgattc tggcgacagc tattttctga atttaaacag tggcgttaat
     1381 ttaggcccat ggagattgcg caacaattca acatggagtc gcagtagtgg ccaaaccgca
     1441 gaatggaaga atctcagcag ctatttgcag cgggcggtta ttccactaaa aggcgaactg
     1501 accgtaggtg atgattatac tgcaggcgat tttttcgata gtgtcagctt tcgtggtgtg
     1561 cagctggcgt cagatgacaa catgctgcca gacagcctga aagggtttgc gcctgtggtg
     1621 cgtggtatcg ccaaaagcaa tgcccagata acgattaagc aaaatggtta caccatttac
     1681 caaacttatg tatcgcctgg tgcttttgaa attagtgatc tctattccac gtcgtcgagc
     1741 ggtgatttgt tagttgaaat caaagaagcg gacggcagcg tcaatagcta cagcgtaccg
     1801 ttttccagcg tgccattact ccagcgtcag gggcgaatca aatacgcggt gacactggcg
     1861 aaatacagaa ccaatagtaa tgaacagcag gagagcaaat ttgcccaggc cacgttgcag
     1921 tggggcggac cgtggggaac gacatggtat ggtggtggac aatatgctga atattaccgt
     1981 gccgccatgt ttggtctggg atttaacctt ggcgatttcg gagcaatttc gttcgatgcg
     2041 acccaggcga agagtacgct ggcagaccaa agcgaacata aaggtcagtc atatcgtttt
     2101 ctgtatgcca aaacgctcaa ccaattgggc actaattttc aattgatggg ctatcgctat
     2161 tcgacgtcgg gtttctacac cctttccgac accatgtata aacatatgga tggctacgaa
     2221 tttaatgacg gtgatgatga agatacgccg atgtggtcgc gttattacaa tttgttttac
     2281 accaaacgtg gcaaactgca ggtcaatatc tcccagcaat taggcgagta cggttcgttt
     2341 tatttaagtg gtagccagca aacttactgg cataccgatc aacaggatcg gctattacag
     2401 tttggctaca acacgcaaat taaagatctc tcgctggggg tttcctggaa ctacagtaag
     2461 tcccgtggtc aacctgatgc tgatcaggtg tttgcactaa atttttccct gccgctcaat
     2521 ctgttgctcc ccagaagtaa tgatagctat accaggaaaa aaaattacgc ctggatgacc
     2581 tctaacacca gtatcgataa cgaagggcac attacacaaa acctgggttt aacggagaca
     2641 ctactcgatg acggtaacct gagctacagc gtgcaacagg gatataacag cgaggggaaa
     2701 acggctaatg gtagcgccag catggactac aaaggggcgt ttgcagatgc ccgagtgggc
     2761 tacaactaca gcgataacgg cagtcaacaa caactgaact acgctctttc aggcagttta
     2821 gttgcccatt cacagggcat taccctgggg caatcgctgg gggaaactaa cgtgctgatt
     2881 gcagcaccag gcgcagagaa tactcgtgtg gcgaacagca ccgggctgaa aactgactgg
     2941 cgcggatata ccgttgtccc ctatgccact tcttatcggg aaaatcgaat cgcacttgat
     3001 gcggcatcgt taaaacgtaa cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc
     3061 aaaggggcgt tggttctggc ggagttcaat gcccatgcgg gagcaagggt attaatgaaa
     3121 acatcaaagc agggtatacc gctgcgtttt ggcgcgatag cgacgctgga cggcatacag
     3181 actaatagcg gcattattga tgatgatggc tcgctctata tgtccggttt gcccgctcag
     3241 ggagcgataa ctgtacgctg gggcgaagct cccgatcaaa tttgtcatat cagttaccag
     3301 cttaccgaac aacaaattaa ctctgcgatt acgcgaatgg atgccatatg cagataatct
     3361 ttggagaaaa atgcgtgtta ttactacgac tattttttgc cgccgtctta atgctatggt
     3421 gcgctcaaac tgctgcttat agcgggcagt gtcataccac tcaggggaat ccgtatattg
     3481 gcgtcaattt tggcgttaaa accctggagg aagaagaaaa tacggctgga gtagttaaag
     3541 acaaatttta tcagtggaac gaatcgaatg attattatgt ttcctgtgat tgcgataaag
     3601 acaatgtcag aagtggccga tgggcattcg ccgcggattc accgttagtc tatttaggcg
     3661 acaactggta caaaattaat gactatcttg ccgccaaagt tttattgcag gttaaaggca
     3721 gttctcctac tgcggttcct ttcgaaaacg tgggcacagg ggcagataca cgatggcata
     3781 tttgcgatcc cggcggtcaa cgtttaggtg gccagggggc tagcggtaat agcggtagct
     3841 tttccctgaa aatattgcag ccgttcgttg gttcggtcgt tattcctcct atggcgctgg
     3901 cgcgattatt tgaatgctac aacatacccg caggtgattc ctgcacgact acaggtacac
     3961 cggttttagt gtattacctg tctggtacga tcaattcact tggctcatgt tccgtcaatg
     4021 ccggagagac tatcgaggtc gatctgggcg acgtatttgc ggctaacttt cgtgttgtag
     4081 ggcataaacc ccttggggcc agaacggcag aactcgcaat tccagtcagg tgtaacacgg
     4141 gaaacgaagg gttagttaac gtcaacctga gtctgacggc aacctcagac cccagctatc
     4201 cccaggcgat taagacgtca cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca
     4261 acattatttc ccctgctggt gggacactac cgctctctat tcctgatgat gcagacagta
     4321 tcgcgcgaat gaatgtctat ccagtcagca cgacaggcgt accaccagaa accgggcgat
     4381 ttgaagccac ggcaacggtg agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa
     4441 aacgatatat cagtacgtta ttttgttctt tagccttctt aacatccatg tcgggatggc
     4501 tgggcctgaa caagtcagta tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt
     4561 cgccaccaaa agtgcattac aaaatattca tattggcgat tttaatatca gtgattttca
     4621 ggccgcgaat accgtaagca ctgcagctga tttgaatatc gatatcaccg gttgtgccgc
     4681 tggtattact ggcgcggacg tcctttttag cggcgaggct gacacccttg ccccgacact
     4741 gcttaaacta actgacacag gcggaagcgg tggtatggca acggggattg ccgtgcaaat
     4801 tcttgatgcg caaagtcagc aagaaatccc gctcaatcag gtccagcctc ttacgccctt
     4861 aaaagccggg gataacacac tcaaatatca acttcgttat aagtccacaa aggcgggagc
     4921 aacgggcggt aatgcgacgg cggttctcta ttttgatctg gtttaccagt gaaggagtga
     4981 tgttgaaacg gataatctgg attctgtgct tactgggatt aacggggagt agtgagttat
     5041 ttgctcatga tggcacggtc aacattagcg gatcgtttcg tcgtaataca tgcgtgctgg
     5101 cacaggatag caagcaaatc aatgtgcagt taggcgatgt ctcgctgaca cgtttttctc
     5161 acgggaatta tggcccggag aaatctttca tcattaattt gcaggattgc ggaacagatg
     5221 tcagcacggt tgacgtcacc ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt
     5281 ccattgaaag tggtatggat gcagccagcg ggctggcaat tgcgattctg gatgatgcga
     5341 agatattgat tccgcttaac caggcgagta aagactacag cctacatagc ggtaaggtgc
     5401 cattaacttt ttatgcgcaa ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga
     5461 atgccagcgc gacatttgta cttcattatg actaatacgt ggaacagatt gacgctcttg
     5521 attttcgccg ttttatcgct gctggtggcg ggtgaattgc gggcaggagt ggtggtcggc
     5581 ggaacgcgat ttatctttcc ggcagacaga gaatcgatat ctattttact gactaatacc
     5641 agtcaggaat cctggcttat taacagtaaa atcaaccgcc caacgcgttg ggccgggggg
     5701 gaagcgtcga cagtgccagc accattactg gccgctccgc cacttattct cctgaagccc
     5761 ggtactacag gcacgttgcg cttgctgaga acggaaagcg acatcttgcc tgtggatcgc
     5821 gaaacgctat ttgagttaag cattgccagc gtgccatccg gcaaagttga aaatcagagc
     5881 gtaaaagtgg cgatgcgctc ggtatttaaa ctgttctggc gacccgaagg tttgccgggc
     5941 gacccgctgg aagcttatca acaattacgc tggacacgga attcgcaggg tgtacaactc
     6001 actaacccaa cgccttacta cattaacctg attcagttga gcgtaaatgg taaagcgtta
     6061 agtaatgcgg gagttgtgcc gcctaaaagc cagcgtcaga caagctggtg tcaggcaatc
     6121 gcaccgtgtc atgtcgcctg gcgggcgata aatgattacg gcgggttgag tgcaaagaag
     6181 gagcaaaatc tgccctgaaa caggttcgga aaacgtttgc gttttttttg ccgcaggtca
     6241 attccctttt ggtccgaact cgcacataat acgcccccgg tttgcacacc gggaatccag
     6301 gagagttcat gtactacccc ttcgttcgta aagccctttt ccagctcgat ccagagcgcg
     6361 ctcatgagtt tacttttcag caattacgcc gtattacagg aacgccgttt gaagcactgg
     6421 tgcggcagaa agtgcctgcg aaacctgtta actgcatggg cctgacgttt aaaaatccgc
     6481 ttggtctggc agccggtctt gataaagacg gggagtgcat tgacgcgtta ggcgcgatgg
     6541 gatttggatc gatcgagatc ggtaccgtca cgccacgtcc acagccaggt aatgacaagc
     6601 cgcgtctctt tcgtctggta gatgccgaag gtttgatcaa ccgtatgggc tttaataatc
     6661 ttggcgttga taacctcgta gagaacgtaa aaaaggccca ttatgacggc gtcctgggta
     6721 ttaacatcgg caaaaataaa gatacgccag tggagcaggg caaagatgac tatctgattt
     6781 gtatggaaaa aatctatgcc tatgcgggat atatcgccat caatatttca tcgccgaata
     6841 ccccaggatt acgcacgctg caatatggtg aagcgctgga tgatctctta accgcgatta
     6901 aaaataagca aaatgatttg caagcgatgc accataaata tgtgccgatc gcagtgaaga
     6961 tcgcgccgga tctttctgaa gaagaattga tccaggttgc cgatagttta gttcgccata
     7021 atattgatgg cgttattgca accaatacca cactcgatcg ttctcttgtt cagggaatga
     7081 aaaattgcga tcaaaccggt ggcttaagtg gtcgtccgct tcagttaaaa agcaccgaaa
     7141 ttattcgccg cttgtcactg gaattaaacg gtcgcttacc gatcatcggt gttggcggca
     7201 tcgactcggt tatcgctgcg cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt
     7261 attctggttt tatttttaaa ggtccgccgc tgattaaaga aatcgttacc catatctaat
     7321 tatttcttcg aattcctctt tataagataa tcagggcttt attttcagcc ctggttgttt
     7381 tatattcatc gctgttgctt atttagacat tttgtacttt tattgttgag gttattaagc
     7441 gaagcgacaa tggattgtgt tgctgcggtt ttatagggta ggggagaggc agatgcgaat
     7501 taaaccagac gataactggc gttggtatta cgatgaagag cacgatcgaa tgatgctcga
     7561 tttagccaat ggtatgctat ttcgctcacg ttttgcgcgc aagatgttga ctccagatgc
     7621 cttttctccc gcaggctttt gcgttgacga cgccgcgctc tatttctctt ttgaagaaaa
     7681 gtgccgcgat tttaatttat ctaaagaaca aaaagccgaa ctggtgctga atgcactggt
     7741 agcaattcgc tatctcaaac cgcaaatgcc gaaaagctgg cattttgttt cccatggtga
     7801 aatgtgggtg cccatgccgg gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca
     7861 ggtcaatttg ctggtcgttg aatctggcga aaatgccgca ctatgcctgc tggcacaacc
     7921 ctgcgttgtt attgcgggtc gcgcgatgca gttgggcgac gccattaaaa tcatgaacga
     7981 caggctgaaa ccgcaggtta atgttgacag cttcagcctc gaacaggcag tctaacgcgc
     8041 caacttaagt gcagtcttcg gtacacagct acagcaaaga atggtgccat catcgcccat
     8101 tgctgatttt tttagcggcg tgacttcgcc ttctaaaagc tgaacacggc aacttccaca
     8161 aatgcccgcg cggcaagaat aagggatacg aatcccctga ttttctaatt gctccagcaa
     8221 cacctgttgg ttatttccac gaaatgcctg tccctgccag tcaatatcta catttgcgtc
     8281 cggttgttgc gtgatgttgg cggtatcatc agcggcagct gcgccgtaaa tttttgccgg
     8341 agccgttgcc agaatttcca cctcatcgcc aacgcgaatc acgccgctat tacgggcaat
     8401 taaattctga ccaaaatcga catcgccgtt atcctgggca gtgcggaaag attgcaatgt
     8461 ttttaatggt tcgcctgccg gatgtttttg ccctttttct gggctgacgg tggtgaaaat
     8521 acagcggcta caaggtttaa ccacatcaaa caccacatca ccaatgcgaa tcactttcca
     8581 gcgatcttct tcccaggctg acgcgccgga aaccaccaga ttggggcgga attgctccat
     8641 ttttacactg gccggacaac gttgttggag atcacgtaac gaggcttcgt tagcaagaag
     8701 gtaaggatag ccatcagcaa atgacagagg tacagtgttg tggcgtttca cgcgccgggt
     8761 catttgtggc cccacccagc gtaattgcac ttcgcgggag aaaaatccac ttagccattt
     8821 gttgatcgcg cctggcgcaa ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc
     8881 gtcttgtgtg gcgaaatcag caaaacgaac atatgcgcta ctgccatctg gtgcggtgag
     8941 atgcaagcca tcatgcacag gtgaaggagt aaaccgtacc atttgcggaa actggcgagc
     9001 agtaataaac gtaccatcag gttccgtgat cataaagatg cgatcgaagg ccagaccact
     9061 cacatctgcc agggtatgtg taagaccaat gccgcgcatc gatttaacag gatgaataaa
     9121 aagccgggtt aatgtcgcca cggtgcggtc ctcaaatgaa aataagccct caactttatg
     9181 acatgacgcg cttattagct ataatgcgca acaattttct tagcgcctgt cccgttaggc
     9241 tattttattt gtcagtttgg cccagggaag tgctcgaaat cctcacgtac ttgtgtacgc
     9301 tccggtttct ccgcgctgtc catgtccaga ctgactgcaa caattacgcc tactgcggta
     9361 ggctcttaga gtaaaagtga cgatatgaat tctctgtttg ccagtacggc ccgtgggctg
     9421 gaagagctgt taaaaactga actggaaaac ctgggggccg ttgaatgcca ggtggttcag
     9481 ggtggggtcc atttcaaggg cgacacacgg cttgtttacc agagcctgat gtggagccgc
     9541 ctggcctcgc gtattatgtt gccgctgggc gagtgtaagg tttacagcga tttagacctc
     9601 tatctcggtg ttcaggcgat caactggaca gagatgttta atcctggcgc gaccttcgct
     9661 gtccacttca gtggtttgaa tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg
     9721 aaagacgcga tcgtcgatgc tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc
     9781 gatgcgccgg atatccgcgt taacgtctgg ctgcataaag aaaccgccag tatcgctctt
     9841 gatctgagtg gtgatggttt acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg
     9901 atcaaagaaa ccctggcagc cgctattgtg atgcgatccg gctggcagcc aggaacaccg
     9961 ctgctcgatc cgatgtgtgg ttccggtacg ttgctgattg aagcggcgat gctggcgacc
    10021 gatcgcgcac caggcttgca ccgtgggcgt tggggcttta gcggctgggc gcagcatgat
    10081 gaagctatct ggcaggaagt gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct
    10141 gagtatagct ctcattttta cggttcggac agcgacgcac gggtgattca acgtgcacgc
    10201 actaacgccc gtcttgcggg gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg
    10261 caactgacca atccgctgcc gaaagggccg tacggcacag tgttgagcaa cccgccatac
    10321 ggggaacgtc tggacagcga accggcgctg attgcgctgc atagcctgct gggtcggatc
    10381 atgaaaaacc agtttggtgg ctggaatctc tctttgttta gtgcctcgcc ggatctgcta
    10441 agctgcttgc agctgcgtgc agacaaacag tacaaggcga aaaacggccc gctggactgc
    10501 gtacagaaaa attaccacgt tgccgaaagc actccagaca gcaaaccggc gatggtagcg
    10561 gaagactacg ccaaccgcct gcgtaagaac ctcaaaaaat tcgagaagtg ggctcgccag
    10621 gaaggaattg aatgttaccg cctgtatgac gccgatctac cggaatataa cgttgccgtt
    10681 gaccgttatg ccgactgggt ggtggtgcag gagtatgcgc cgccaaaaac tattgatgct
    10741 cacaaagcgc gtcagcgtct gttcgatatt atcgctgcaa ccatttcggt actggggatt
    10801 gcgccaaaca aactggtgct gaaaacccgt gaacgccaga agggcaaaaa tcagtaccag
    10861 aaactgggcg agaagggcga gtttcttgaa gttaccgaat ataacgctca cttgttggtg
    10921 aacctgacgg attacctcga taccggtctg tttctcgatc accgtatcgc ccgtcgtatg
    10981 ctcggccaga tgagcaaagg caaagatttc ctcaacctgt tctcttatac cggcagcgcc
    11041 accgtgcatg cgggattagg cggtgcacgc agcaccacca ccgtggatat gtcgcgtact
    11101 tatctggagt gggcagaacg caacctgcgt ctgaatggcc tgaccgggcg tgcgcatcgc
    11161 ctgattcagg ccgattgcct ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc
    11221 tttatcgatc cgccaacctt ctctaactca aaacgaatgg aagatgcgtt tgatgttcag
    11281 cgcgatcatc tggcgctgat gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc
    11341 atgttctcga acaacaaacg tggcttccgt atggatctcg gcggcctggc aaaactggga
    11401 ctgaaagcac aagaaattac gcaaaaaacg ctctcccagg atttcgcccg taaccgccag
    11461 atccacaact gctggctgat taccgcagcc tgaaaggaat agtaatgtca ttaatcagta
    11521 tgcatggcgc ctggctgtcg ttcagcgacg cgccgcttct cgataacgca gaactgcata
    11581 tcgaagataa tgaacgtgtt tgtctggtag gccgcaacgg cgcaggcaaa tcgacgttaa
    11641 tgaaaatcct caaccgtgaa caagggctgg atgacggtcg cattatttac gagcaagatt
    11701 tgattgtagc tcgtctgcaa caggatccgc cgcgtaacgt tgagggtagc gtttatgatt
    11761 tcgttgccga aggcattgaa gaacaagcgg aatatctgaa acgctatcac gatatttcgc
    11821 gcctggtgat gaacgacccg agcgagaaaa atctcaacga actggcgaag gttcaggaac
    11881 agctggatca ccacaacttg tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac
    11941 tggggttaga tcctaatgtt gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg
    12001 cattaggacg cgcgctggtg agcaatccgc gcgtattgct gctggacgaa ccgactaacc
    12061 atctggatat cgaaaccatc gactggctgg aagggttcct taaaaccttc aatgggacga
    12121 ttattttcat ctcccacgac cgttcgttta tccgcaatat ggcgacgcgc attgttgatc
    12181 tcgatcgcgg caagctggtg acctatccgg ggaattacga ccagtacctg caggaaaaag
    12241 aagaagccct gcgcgtggaa gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg
    12301 aagaagtgtg gattcgccag gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac
    12361 gcgccctgaa agcaatgcgt cgcgagcgtg gtgaacgtcg cgaagtgatg ggtaccgcaa
    12421 agatgcaggt ggaagaggcc agccgctccg gtaagatcgt tttcgaaatg gaagacgttt
    12481 gctaccaggt tgacggtaag caactggtga aagatttttc ggcccaggtt ctacgtggcg
    12541 acaaaattgc cctgattggt ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc
    12601 tcggtcagct tcaagcggac agcgggcgta ttcacgttgg caccaaactg gaagtggctt
    12661 atttcgatca gcaccgcgcg gaactggatc ccgataaaac ggtgatggat aaccttgccg
    12721 aaggtaagca agaggtgatg gttaacggca agccacgcca cgtattgggc tatttgcagg
    12781 actttctgtt ccatccgaaa cgggcgatga cgccggtacg tgcgctttct ggcggtgagc
    12841 ggaaccgctt gctgctggcg cgtttgttcc tcaaaccaag caacttattg attcttgacg
    12901 aaccgaccaa cgatcttgat gtcgaaacgc tggaactgct ggaagaactg atcgacagct
    12961 atcagggcac ggtattgctg gttagccacg atcgtcagtt tgtcgataac accgttacag
    13021 aatgttggat cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg
    13081 cccgtggtca gcaagagcag tatgtggcgc tcaaacagcc tgcggtgaaa aaaaccgaag
    13141 aagccgccgc ggcaaaagca gaaactgtaa aacgcagcag tagcaaacta agctataaat
    13201 tgcagcgcga actggagcag ctaccgcaat tgctcgaaga tctggaggcg aagctggaag
    13261 ccctacagac gcaggtggcg gatgcttcct tcttcagtca gccacatgag cagacacaaa
    13321 aagtgctcgc cgatatggct gctgcagagc aggagctgga gcaagccttt gaacgctggg
    13381 agtatcttga agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt aaacagagac
    13441 agctgacaac ggtaaaattg cctgatgaac tgggtttatc aggcatgaaa accaacactt
    13501 tgttagtcat ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg
    13561 ccgcgaagca catcctgtgc tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc
    13621 atggtcagaa agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc
    13681 ctcggcagcg tcccaccgcc tatgcgttgg ctgcactgtt catgctgttg ctgtccaact
    13741 tgtttccttt tgtgaatatg aacgttgcag gagttaccag tgaaattaca ttactggaaa
    13801 ttcccggcgt gcttttttct gaggactacg ccagcctcgg cacctttttc ctgttgtttg
    13861 tgcaactggt tcccgcgttt tgtctgataa ccattctgtt actggtgaat cgcgcggaat
    13921 taccggtccg tttaaaagag caactggcac gggtgctttt tcaactcaaa acctggggaa
    13981 tggcggagat tttcctcgct ggtgtgctgg tcagtttcgt taaactgatg gcttacggca
    14041 gcattggggt aggcagcagc tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg
    14101 cttttcagtg cgttgatcgt cgctggttat gggacgacat cgccccgatg ccagaactgc
    14161 gccagccgct aaaaccaggc gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt
    14221 gtacggcaat ccttcctgct gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg
    14281 ttcggcgtag aaacagcctg cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt
    14341 atcttccggc taatattttg cccatcatgg tgacggattt attaggctcg aagatgccgt
    14401 cgacgattct cgctggggtc attctgttat ggagcgaagg ctcttatccc gtcgctgcgg
    14461 tgatctttct ggccagtatt atggtgccaa cgttaaagat gatcgccatc gcgtggctgt
    14521 gttgggatgc caaagggcat ggcaagcgcg acagtgaaag aatgcatttg atttatgaag
    14581 ttgttgagtt tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg
    14641 cgctggtgcg tatgggaggt ttaatgagta tttatccggc aatgggtgca ttaatgtttg
    14701 ctttagtcgt cataatgaca atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg
    14761 atcgtcaacc tgaatcagag catgaggagt cctgacagta tggaatctaa taatggggaa
    14821 gccaaaatcc agaaagtgaa gaactggtct cccgtgtgga tatttcctat cgtcacggcg
    14881 ctcattgggg cctgggttct tttttatcat tacagccatc agggaccgga agtgaccctg
    14941 atcaccgcga atgcggaagg aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt
    15001 gacgtcggcg tggttgaaag cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa
    15061 gcgcggctga attccggtat ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg
    15121 aaaccgcaga ttggtcgaga agggattagc ggtctgggaa ccctgctgtc tggggtttat
    15181 atcgaactgc agccaggcgc gaaaggcagc aaaatggata aatacgattt gctggactcg
    15241 ccaccgttgg ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc
    15301 gggcagctct cgccaggaga tccggtgctg ttccgtggct atcgggtagg ttcggttgaa
    15361 accagcacct tcgatacaca aaaacgcaat atcagctatc aactgttcat caatgcacct
    15421 tatgaccgac tggtgaccaa caatgttcgc ttctggaaag atagtggcat tgcggttgat
    15481 ctgacgtcag cagggatgcg tgtggagatg ggctcattga caacgctgct gagtggcggt
    15541 gtcagctttg atgtgccgga aggtctggat ttagggcagc cagtggcacc gaaaacagct
    15601 ttcgttttgt atgatgatca gaagagcatt caggattcgt tgtacaccga tcacattgat
    15661 tatctgatgt tctttaaaga ttcggtacgc ggtctgcaac cgggagctcc ggtagagttc
    15721 cggggtattc gcctgggtac cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag
    15781 acatttaacg atgattaccg tattccggta ctgattcgta tcgagccaga gcggctgaaa
    15841 atgcagcttg gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt
    15901 ttacgcggat cgctgaaaac cggaaacctg gtcactggtg cactgtatgt tgatctcgat
    15961 ttctatccaa atacgcctgc aataaccggt attcgtgaat ttaatggtta tcagattatc
    16021 ccgaccgtta gcggcggcct ggcgcaaatc cagcaacgac tgatggaagc gttggataag
    16081 atcaacaaac tgccattgaa tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt
    16141 cagcgcacaa tgaaaaacct gcaaacgacg ctggatagca tgaacaagat cctcgctagc
    16201 cagtcgatgc agcagttgcc gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc
    16261 atgcagggct tccagcctgg ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc
    16321 cttgatcagg tgttgcgaga actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac
    16381 gcgctggtat ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc gaaacaatga
    16441 aaaagtggct agtgacgatt gcagcactgt ggctggccgg atgcagctcc ggcgaaatta
    16501 ataaaaacta ttaccagtta cctgtggtgc agagcggtac acaaagtacc gccagccagg
    16561 gcaatcgtct gttatgggta gagcaggtca ctgttcctga ctatctggcg gggaatggtg
    16621 tggtttatca aaccagtgat gtgaagtatg tgattgccaa taacaacttg tgggccagcc
    16681 cgttggatca acagttgcgc aacaccctgg ttgccaacct gagtacgcaa ctgcccggct
    16741 gggtggttgc ctcccagcct ctgggaagcg cccaggacac gctcaatgtt accgtcacgg
    16801 agtttaacgg tcgctatgat ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc
    16861 agggacaact gatcaaacgt ccgttccgtc tggaaggggt gcaaactcag gatggttacg
    16921 atgagatggt taaagtactg gccagtgtct ggagtcagga agccgcttct attgcgcaag
    16981 agataaagcg tctaccttaa ttataaagat ttgtaaatat aaccgcctcc ggtatgttgc
    17041 ctgaggcggt ttttttgtct ctaacgtgcg gaaaaagttg ttcctcttca cattttttgt
    17101 acaaccggca tacccgtgta gctcacaaat atgacagtgg cgtgaatttt gcgcattgac
    17161 ggcagttatg attcgcggta ttgcttaact gtgattgcac atttagtaat cactgttttc
    17221 ttttccacca gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc
    17281 tggaacgggc acatcaacgt ggttatcagg ccggcatcgc cggacgctca aaagaaatgt
    17341 gtccctatca gacgctgaat caaaggtcac aatggctggg aggctggcga gaagccatgg
    17401 cggacagggt agtaatggcc tgattctgtc tctttaaaaa gaaacctccg cattgcggag
    17461 gtttcgcctt ttgatactct gtctgattat aatcagaagg cagacgtatc ctggaacaga
    17521 ccgactttca ggtcgctggc ggtatagatc agacgaccat caaccagcac ttcgccatcc
    17581 gccaggccca taatcagacg acggttaaca atgcgtttaa agtgaatacg gtaggtcact
    17641 tttttcgctg tcggcagtac ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg
    17701 cctttacctt cgccgcccag ccagccgagg tagaacccta ccagctgcca cattgcgtcc
    17761 aggcccaggc atcccggcat aaccggatcg ccaataaagt ggcatccgaa gaaccacaga
    17821 tccggattga tatccagttc tgcttcaaca taccctttgt cgaagttacc acccgtttcg
    17881 gtcattttga ccacacggtc catcatcagc atgttcggtg ctggcaattg cgggccttta
    17941 gcgccaaaca gttcaccgcg accagaggca agaaggtctt cttttgtata ggattcgcgt
    18001 ttatctacca tgttctctgt aagccttatt ttattgaagc acgcaggata gctaacacgt
    18061 gtacgctgaa caagtccgat cagttcggaa taaaccagtt cagccaacgt aatggccatg
    18121 gaaaacggtg acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg atggtttgca
    18181 tcagcgtcgt ttggccttcg ccatcccaca ccagatttaa taataacggc agtgcgtcag
    18241 tcacatcgtc tactgcccag atggtgaatt tgccttcttc taccgctttc accagttcac
    18301 tgtgaagact taaatggcga acgttagctg tggggataat gacaccttgt ttcccggtta
    18361 actcacgttg ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg
    18421 gctgggcgcg accgaactga tcgactgaac ctgtgatagc gatactctga ttcaccggca
    18481 catcggcgag ggcgcttatc agggcgcaga gttcagccat cgaggcacta tctccatcaa
    18541 cttcactgta tgactgctca aatgtcagcg atgctgagaa ggggatctgt tgctcaagct
    18601 gtagttccga catcaggaac gcttgcatga tcatcatccc tttcgcatgg atattgccgc
    18661 caagctccgc tttgcgttcg atgtcggtga attcaccatc gccaatatgc acaacgcagc
    18721 taatgcgaga aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg
    18781 cgttaatttg cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc tgaaggatct
    18841 catcctgcat acgttcagcg aggaaacctt cgcgccattc acgctgctgc agcattaagt
    18901 ttagctgctc gccggagaag gtgtcgccat cacacaggga ggcgacctct ttacactggc
    18961 ggaggatcca ctgcgggcta agcggaagtg tttcttgttc accggtgtag cgtgctgctt
    19021 cgcggataag taccggccag gcatccgctc ccggtgcagg taagtgatta tgtctggcgg
    19081 taaatgtcac ccagcgacac cactgggtta ctgactctgc atcgacaatc tgcagagtat
    19141 cttcaaattc gctataaata gcctgctctg aaagctctgg ctccatctcc tggaaatcag
    19201 acaatgattc gcgttcgcct accagaatga ctttcagctt caatggcatc gaaggcacag
    19261 agacggggag agggcgcgac tcatcaaacg caacccagtc aaaacgctcg cggttaacga
    19321 tatttttcag ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa gagatgatga
    19381 gaataccgcc atttgcctga tgcaccagac caggctgcag ggtaatgtcg ccattaaact
    19441 ggcgcaggca gccaaagagt tgctccgctt ctacccagtc ggcagcgaca acttgcgtta
    19501 aagtcgcaaa attatcatct gcactcactg cgtgacgtaa gcggatggag tggccggaaa
    19561 cctcatagtg accgcccacc agttggcctg catcgctttg tagcgtacgc gcggcattgg
    19621 caataagatt cagatactca gactcttccg gggccttcgc cagcataaaa gaggaggatg
    19681 ctcgcgtatg cagcaactgc tccagtgcga attgcagtcg cggttgagta tcactgaata
    19741 aaggatcgtt ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag ctatcggtat
    19801 caggaaccag gtcacgccat gcaagtttcg taatggtcaa agttgatgtt ttttagtctg
    19861 ttgtcaaagc cgcgattata ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa
    19921 tactcctggc atgggcgtta aagctcacag gatggagatt cttttcttca ctggcctaaa
    19981 aagctgatat tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa
    20041 cttgaaaatc ttgaaagcgg ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg
    20101 gagttaatca cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc
    20161 tcgctcgaaa atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa
    20221 ctggtcaatc gcatgaagca aaccattcgg gcaagacgaa agcgccattt taatgcggaa
    20281 caccagcata cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct
    20341 ggtcttgcgc agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct gattgaagat
    20401 gcggaaaaca aagagaaata cgcgaataaa atgtcttctc tgaagcagga tctgcaggca
    20461 ttgctgggta aggaataact gacgaaagtc agttcaattt actaaaggca aaaaaaaccc
    20521 cgcagcagcg gggtttttct accagacgag aacttaagct tgcggctgag ttacaacgtc
    20581 tttgatacct ttaacttcga tctctacgcg acgatccgga gccaggcagt cgatcagtgc
    20641 agcacgctgt ttcacgttgt cacaggtgtt gccagtaacc gggttggatt cgcccatacc
    20701 acgtgcggag atcttgtctg ccgggatacc tttggagatc aggtaatcaa caacagactg
    20761 agcacggcgc tcggacagac cctggttgta agcgtcagaa ccgatgcggt cggtgtaacc
    20821 cagaacaact acggaaccgt ctttcggatc caggttgctc agctggctgt acagctgatc
    20881 cagagcagcc tgaccttccg gtttcagggt tgctttgttg aagttgaaca gaacgtcaga
    20941 cttcagagtg aagtgcttgg tctgtacttc cggtgccgga gctggagccg gagcaactac
    21001 tggagctgct tcgccctgac cgaaacggta ggaaacaccc aggctcagca tgccgttgtc
    21061 cggacgagtg ccgatggtgt gtgcgtcacc gatgttgttg gtccactggt attccagacg
    21121 ggtagcgatt tcaggagtga tcgcgtactc aacaccgcca gcgaagaccg gagaaacgcc
    21181 ggtgtcgtgg tttttaccat aaacgttgga tttagtgtct gcacgccata ccataccacc
    21241 cagacgagtg tagatgtcca ggtcgtcagt gattgggtaa cccagtttag cggtcagttg
    21301 aacgccctga gctttgtatg caccgttttc aacgctgcct ttgtacggca tacgacctaa
    21361 ccagtcgtaa cccatttcaa agccaacata cgggttaacc tggtaaccac caaaagcacc
    21421 agcgcccagt tggttttcat gggtcgggcc attgttgttg atgaaaccag tgtcatggta
    21481 ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag cggcctgcgc
    21541 tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt tcattttttg
    21601 cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca cggttaaatc
    21661 cttcaccggg ggatctgctc aatattaact ctaccgatat cttcggctta tgccgagcac
    21721 ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac tccgtcaggc
    21781 atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt tttgtcagcg acaatttaca
    21841 gaagaaaatc gcggaaaccg cttcagacaa gcctccgcaa ggaaaattag tcacgactga
    21901 aagcattggc tgggcgacaa aaaaagttcc aggattaatc ctaaatttac ttaatgatac
    21961 aaattagagt gaatttttag cccggaaagt tgtctcgtgg cgtgagagga tgcgcttacc
    22021 ggacgcataa taaaccccat agcgttacct tcatttgccg catcaacaag ttcagcatgc
    22081 tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca cactgtaatt gcccgtgcgt
    22141 aaagcgcgaa ccattgactc cacagtgtgg caaggggaga gctggctaat ctgcattact
    22201 ttcgttaagg gtagcccaga tgcctgaacc cattcccgac tcagtttttg ttgcggtgtt
    22261 aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa cagtagaagt
    22321 tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc ggctgtagtg
    22381 ttttccgtag agacacgcgc aattttactt gctgcggatg agaacgacga agaacgatgt
    22441 gcatagcctg aagtgtacat aatcaatcca gcccctgtga gttactgtat ggatgtacag
    22501 tacatccagt gacaacaaag atcaacccta ttttcggaaa gagcctcgca aattttgtcg
    22561 ttggtgacgg gaaaacataa attaatcttg ccccttaaga ataagttgcc tattttcgta
    22621 gttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa ggaattttat
    22681 gaaaagcctc tcctataagc ggatctatta atcacaagaa tacctggcaa cgttgggcac
    22741 aattgaatac cgatcattgt ttggcagtta cagcctgacc gttgacgaca cggtgtttgc
    22801 gatggtttct gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac agtactgtgt
    22861 aaaacatccg cctgtctggc tgacatataa aaagtgtggc cgatccgtta ccctcaatta
    22921 ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc tgtcgaaata
    22981 ttctctcgat gcagcgctga aagagaaaag cacgcgcaat acccgggaaa gactgaaaga
    23041 tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggga ttaaggatgt
    23101 acgggcgtta cgtatacttg gggcaaaaat gtgttggttg cgactgcggc agcaaaacag
    23161 tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc attatcggca ttcatgaagc
    23221 tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg gctgactctc ttacgccgaa
    23281 acaggagttt cctgcggaac ttgagtaatc tggcgttgca gacgaccaat ctcaggcagt
    23341 aatgcaatca ataatccaac ttgttgtacg accagaggtt ctttgctgtc tgcccgtggt
    23401 tcaagttgct gcatccgctg tttcaggcta gctaatgcct cattgacgcg ttcttcatca
    23461 gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg cgtcatcaag aaacgccaga
    23521 atttcaggat tagttaactg ctcccggtga gcaccgaggg ctgagatata gctggtaaac
    23581 gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa tttgcggggt aacgttcggc
    23641 tcgctggaca tatttgatac caccgacgcc agctcagcat cacggttgtg tgcatcgcgg
    23701 cgggcaatac gatacgccag acggttatca cgcccctgat ggtattgctc cagtatggca
    23761 tcgagatacc gacagttggc ctctgtggcg cgttcgagca tgcgcggcag attgcgaaac
    23821 tgccagtcag gccagatgta gctcactgcc gcccacgcaa tggcacaacc aatcagcgta
    23881 tcgattacgc gaggtaacgc tacttcaaaa ccttcaccca gtaagttaaa acacagtagc
    23941 accaaaagtg tgatgaacat cgttgcatga gcgtattgca cgttacggaa ggcaaaaaag
    24001 agcacgccgg taataaccag cagcaccagc tgcccttcca gtgatggcac aaaccacagc
    24061 acaggaatgc caatggcgat acctaccagc gtaccaataa tccttaactt caggcggtgg
    24121 cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg tcagcaagat ccaatacccg
    24181 tgatgcattc cggttatttg aatgatggcg tagccgaagc acaacaccag cgacattctt
    24241 accgcatgac ggaagagggc ggattccggc gtgaagtgac ggctaagacg cagccagata
    24301 tcactcaacc cgtgcgggct gtcatcagcg agctcatttt cgtcattatt atggggtagt
    24361 gcctgggcct gttctgattc aattgttgcc agttgggcat caatggcgcg taaattgttc
    24421 agcaaaaatc ccagtgtttt gagtaaatcg gcgggtgcgc cgttatcgcg catccgctcc
    24481 agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg gatcatgttg ataaggctga
    24541 cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc cctgcatcga catcagccgc
    24601 tgaaaacgga acagcacgtc gctgtggcga aaatgttcac gcaatgtttg atactgaata
    24661 tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa agtaataatg cagcgtgcga
    24721 cgcgttcccc gttgaccacg atcgccacgt aagcgggtca gcagcgagag tttcgtctga
    24781 ttcaatgtcg ccatcagcag accgttggcg agagccaaat cgtacagcgg tgcctggctt
    24841 tcatcttcaa tatcaggatc aaacatgcgc gacttgagct caagataacg cgccagttgt
    24901 tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg ggaacagcag atgaccaata
    24961 agtgtcagga cgttgtacca gacggcaccg gccagcagat acatcggctg ctgataccag
    25021 tgctcataca gtgatgttcc caacatagtg taaatggcga tcagcaatgc accgaaggca
    25081 attgttgcat agcgttgacc cagaccgccg agcaaaatga agccgctggt agagagcgtt
    25141 aagccaatcg caaatagcca gggccaggga aacagcaatt ctactgaggc cgaggcgata
    25201 aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg gtcatcgaga
    25261 tcggtcagcg ccgctgccac catccccagc gttagcggaa tcgtcagttt tacatcaccc
    25321 agccaccacg gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc gtaatacagc
    25381 caggcgctgt tccaggtata gcgtttgagc aaaggactta gcataaaggc catattagtg
    25441 gattattcaa aacgacgacg tgcattggct tcacgcgcag cttgcgctgt ttctaccgat
    25501 accacgcgac gaccaaccgg ccatagtgca atagcggcaa ttttaaagtt cgcaatgccg
    25561 acaggaatgc caatgattga aatacattgt gcgatgcccg ttgcaatgtg catcaggcat
    25621 aaccaccagc caaagaaaat cagccagaaa atattcaata ccgtaccgcc agtattcagc
    25681 agtacatttt tgccagccgg gttcagttca tcgacatgaa tagcttcatt gccataaggc
    25741 accagagaca gtttagtgat ctcccagcag gatcgtgtca gcggtaaggt aaaaatcagc
    25801 acaatactga ccagagtcgc caacagccag cccagagtgg tggcaaatcc gccaagcaca
    25861 aagttcagaa tgttcaaaac ggtacgcata aaacctcgct ttactgtggt tttcagtaat
    25921 gggcggcaat tgtaacgttt ttttgggctg gagcacgttt tctctgacgg ttacactgat
    25981 aagaaataat ttcgtgtgga tctacagagt catggaactg aaagcgacaa cgcttggaaa
    26041 acgtctggca cagcaccctt acgatcgggc ggtgatcctc aatgccggga ttaaagtctc
    26101 cggcgatcgc cacgaatacc ttattccttt caatcaatta ctggcgattc actgtaagcg
    26161 cggtctggta tggggcgagc tggaatttgt actgccggac gaaaaagtgg tgcgtctgca
    26221 cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat cttgatgctc actggcggcg
    26281 gtggagtggc gagatgagcg aaattgcgtc tggtgtttta cgccagcaac tggatttgat
    26341 tgccacgcgc actggggaaa ataaatggct gacgcgtgag caaacctctg gcgtgcagca
    26401 acaaatccgc caggctttgt cggcgttgcc gttgccggtt aaccgactgg aagaattcga
    26461 taactgccgt gaggcgtggc gtaaatgtca ggcctggttg aaagatattg aaagcgctcg
    26521 gttgcagcat aaccaggcgt ataccgaagc catgcttacc gagtatgcgg attttttccg
    26581 ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg gcagtcgtta atggcgagca
    26641 ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg tcggtgctgg tggcccgtgc
    26701 aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa attttattgc tggcgtttgg
    26761 tcgcaaagcc gctgaagaga tggacgagcg gattcgcgaa cggctacata ccgaagacat
    26821 tactgcacgc acgtttcatg cgctggcgct gcatattatt cagcagggca gcaaaaaagt
    26881 tccgatagtc agcaaactgg aaaatgatac cgctgcccgt catgaactct ttattgctga
    26941 gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag ggctggcggc aatggctgac
    27001 ggaagaaatg cagtggtcag tgccagaagg taacttctgg gatgatgaaa aattacagcg
    27061 tcgccttgcc tctcgcctcg atcgctgggt aagtctgatg cggatgcacg gtggtgcaca
    27121 ggcagaaatg attgccagtg cacccgaaga gattcgcgat ctgttcagta aacgtatcaa
    27181 gttgatggcc ccgttattaa aagcctggaa aggtgcgctg aaggcagaaa acgctgtcga
    27241 tttttcgggc cttattcatc aggcgattgt gattctggag aaaggtcgct ttatcagccc
    27301 gtggaagcat attctggttg atgaatttca ggatatctcg ccgcagcggg cagcgttgtt
    27361 agcggcatta cgcaagcaaa acagtcagac gacgttgttc gccgttggtg atgactggca
    27421 ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc accgctttcc atgaaaactt
    27481 tggtgaaggc gatcgctgtg atttagacac gacttaccgt tttaacagtc gtatcggtga
    27541 ggtggcaaac cggtttattc agcagaaccc aggccagttg aaaaagccgc taaacagctt
    27601 aaccaatgga gacgaaaaag ccgtcacgtt attggatgag agtcaacttg acgctttgct
    27661 ggataagctc tctggttatg ccaaaccgga agagcgcatt cttatcctgg cgcgttacca
    27721 tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc tggccgaagt tgcaaatcga
    27781 ctttatgacc attcatgcca gcaaagggca acaggcggat tacgtcatca tcgttggctt
    27841 gcaggagggg agtgatggtt ttccggctgc ggcgcgggag tcgattatgg aagaggcgct
    27901 actgccaccg gttgaggatt tcccggacgc tgaagaacgg cggttaatgt acgtggcgct
    27961 gacccgggca cgccatcggg tatgggcact gtttaacaaa gagaatccct ctccctttgt
    28021 ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa ccgtaagaaa caggtggcgt
    28081 ttgccacctg tgcaatatta cttcagacgg tccgcgagat aacgctgata atcggggatc
    28141 agaatatcga ccgcgtcgtt gaaatgcggc gactggataa tgaagtctgc cgttgccacg
    28201 ttggtggcga ccggaatgtt ccataccgtc gccagacgca gcaaggcttt cacgtcagga
    28261 tcgtgcggca cggcatttag tggatcccag aagaaaatca atacatcaat tttcccttct
    28321 gagatcaatg cgccaacctg ctggtcaccc cccattgggc cactcaacat cgcgttgacg
    28381 ttcatgccgg ttgcacggga aatcaagtta ccggtagtgc ctgttgcata cagtacgtgt
    28441 tgttccagta acggttgatg ccgttccacc cagctcatca gcatttgttt gcagtgatcg
    28501 tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc gagtcgtcag ttccataatg
    28561 tacatccgta gttaactttc ctacagatta ctgtaagcac ttatcgctgc aagataaaga
    28621 ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga tggttgtttt tccgcccact
    28681 gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa ccaatacttc agccgttgtt
    28741 ctgtgagcac ctgagactgc ggtggaatag catccagctc ggaaacaccc gaaagcagcg
    28801 tactgtcatc tgccatcatc gtggcaaatt gcggtagtga agcgcgtttc gcggatttgt
    28861 tatagcgttc aacctcgacc tcataatcaa tcgtttttgc cgttgaggta atggcgagaa
    28921 tatccagctt taccggaatc ggcgtcgcat cgccatccaa caattcaagg cgcggcgcgg
    28981 catcaaaatg gttagcttcc cgctcattct ccaggcgagg caggcgaaaa ttgacctggt
    29041 tgatgagctg ggtattaaaa ctgaccacca gcggtgggga gatatacagc cgttcttcac
    29101 tattggaaag atgaatcgtc ttctcaacgc gaaacactaa ctgatgcggt ccattatcca
    29161 gttcaatgct gtccgcaccg cgcagcagag agctggaaac cttcttgccg tcgagtacca
    29221 gaagatcgac atcggttgaa agccgcagcg tggtggcaaa aacggatacc ggcaaacata
    29281 atgcaatcaa ggtggtcacg atgccggttt tcataggtag ctcctgtcaa aagaccgccc
    29341 gaataataat gtgtcaaaat ttttctttaa tcatacttac taacatatag acatatttcc
    29401 cgcttttgct ctcattcatt cgtattagct gcatggttgg catgtcggct ttgtcgtaca
    29461 ctttgttaaa cagtctggag gaaataatga aagaaaccga tattgcaggc attttaacgt
    29521 ccactcacac cattgcgctg gtgggggcaa gcgacaaacc cgatcgtccc agctatcgcg
    29581 tgatgaaata tctactcgac caggggtatc acgtcattcc ggtttcgcca aaagttgccg
    29641 gcaaaacgct gctgggccag caggggtatg gcacgctggc agacgttccg gaaaaggtcg
    29701 atatggtcga tgttttccgt aattcagaag cggcgtgggg cgtggcacag gaagctattg
    29761 ccattggcgc gaaaaccctc tggatgcaac tgggggtgat taatgagcaa gcggcagtac
    29821 tggcacggga tgccgggttg aatgtggtga tggatcgctg cccggctatt gagatccctc
    29881 gcctgggcct ggccaaataa aaaatccccg gaaggcaaaa accttccggg gatacgttca
    29941 gggatcagtt acgcagacgc ggggcctgga gttgtttgcg gatggtctgc gccagctcat
    30001 ccatggatgg ctgttcagga tgttcatcct gcaattcgct acttagctgg gcttcagcca
    30061 ggtaggtatg aaccggtagg ccgttatcgt cctccatcac cacgtgatac cacggagcgg
    30121 cgcgaagctc gtcattcacc gccagctcat caggcgacgg ttcagagagc gaataaaccg
    30181 ggtcgatatc cacgaccact ccgagataac ctaacaggga atggcggacc tgctggccga
    30241 taccgaattt gctggcaatc atagtcacct cccgggaaat ctttaccttt gatataaggg
    30301 taaaattcca catttcaagt tacatgacgc gacaggcaaa ccctttcaga tatagccctt
    30361 ccggataggt agcgatcacc ggatgatcgg ctgcctgacg gaactgctct ataaattgta
    30421 catcacggcc ggcatcaatt gcggcatccg cgatgatttt ctgaaataaa tcgctggtca
    30481 tcagaccgga acaggagaaa gtcaggagaa taccgccttc attcagcaac tgaatcgcca
    30541 gcatgttgat atctttatag ccacgacacg cgcccatcaa ctggctttta ttctcaacaa
    30601 acttcggcgg gtccatcacg ataacgtcaa atttttcacc gcgatcgcga taagtacgca
    30661 gcaatttaaa gacatcatca cggacaaact cagccttgct cagatccagt ttgttcagct
    30721 caacgttctg ccgtgcaata tccagcgctt cctgggaggt atcaacgctg acaacctggc
    30781 tgcaaccgcc catcagtgcc gatacggcga aaccaccggt ataggagaaa cagttcagca
    30841 cacgtttatt ttcaacgtag cggcgggtag ccaggcggct atcacgctgg tccaggtagt
    30901 agcccgtttt gtgtccgtgc tgaatatcca ccagcagctt catgccgtgt tcttcaatcg
    30961 gcagcagggc aggcggcaac tcgccggtga cgaggccctg ggtcagctcc atcccttctt
    31021 ttttacgtac cgcaacatcg ctgcgatcgt aaatcgcaca ttccgggtac agcgtttgca
    31081 gggcactaac taatgccgcg cgctgatatt ctgccccagc actcagcagt tgcagcacca
    31141 gaaaattacc gaaacgatcg atagtaatac ccggcaggcc atcagattct ccggcgatta
    31201 aacgatagct gtcgaggcca tctttttgcg ccagccagtc acgccatttt tgtgcttgtt
    31261 gcaaacggcg ggaaaaaaaa gcaatgtcga tagactcaga cgggtcaaac gtccagacgc
    31321 gcgcccggat ttgcgaagct ggtgaataag cgccgcgtgc taaccatttt ccctgatgat
    31381 caacaatatc gatggtttca ccgaggctgg ctttaccttc catgcgggca acggccccgg
    31441 aaaagaccca cggatggcga cgaagtaatg atttttcgcg ccctttggct aacactaaac
    31501 gtacactcat aatttacttt tctgtcgatg caaagaaatg ggcgtcattg tccggagttt
    31561 tccatggatt tgcaacgcgt ccattaagga taaggagagc agcaatgtcg aaagtctgca
    31621 taattgcctg ggtttacggg cgagttcagg gcgtaggatt tcgctacacc acacagtacg
    31681 aagcgaaaag actgggttta accgggtacg ccaaaaatct tgacgatggc agcgtggaag
    31741 tggttgcctg cggtgaagaa gggcaggtgg aaaaattaat gctgtggtta aagagtggcg
    31801 gcccgcgttc tgcgcgtgta gaacgggtgc tcagcgagcc gcatcatccc tcgggggaat
    31861 taaccgattt tcgtatccgt taaatacatt ttaccggttt tggcaggcca gcaattttgg
    31921 tggcttgctt agccggacct ttcggaaaca gtcggtataa atagcggctg ttaccttttt
    31981 cttcgccaaa tttattcgcc atcgctttta ccagcatacg aatcgccgga gaagtattga
    32041 attccagata gaaatcacgc acaaaacgca ccacttccca gtgttccggt gagagcgaaa
    32101 tcccttcgtt ctctgcaatc accactgcca gcggctcact ccactggctg ctttctttga
    32161 gatagccttc ggtatccgtt tctatctctt taccttcgaa gatcagcata attattacta
    32221 ccttaatcag actggcggca gtgtaacaaa aaacaaagcc ccgcataaag cgaggctatg
    32281 aaagtgttag cgggtgagat taatcgcggc tggcgaagcc cagaatgctc agcaggctga
    32341 cgaagatgtt gtacagcgaa acatacaggc taaccgtggc acgaatatag ttcgtttcac
    32401 cgccatgaac gatgttgctg gtttcaaaca aaatagcgcc agaggagatc aaaatgaaga
    32461 ccgcgctgat cgccagatgc agagcaggca gctgcaggaa gatattagca accataccaa
    32521 tcagcaccac cacaataccc gccatcagca taccgccgag gaacgacata tctttgcggg
    32581 tggtcagcac atatgcagag cagcagaaga acactaacgc cgttccgccc agtgccatag
    32641 cgattacgtc acccattccg gcagacagat aggtgttcag aataggcccg aggatataac
    32701 ccagaaaacc ggtaaaggcg aatgcggaga taatcccggt cggcttattc gccgttttat
    32761 aggtcaggaa catcaaacca tacataccca ccagcgtcag aatcagaccc ggagatggca
    32821 gcatcagcac cgtactggca gttgcggtaa ttgccgaaaa ggccagcgtc aggctcagca
    32881 gaaaataggt attacgcagc accttatggg tgctaagcag tgatgtacgg tcatgtgaag
    32941 aactaacaat acgatccatt gagtcactct ctatgacaga tgtaattaat taagcagcat
    33001 aatgataatg cgtaagggca cccagaagtt ttacccatct ttacgcattt gatctggaac
    33061 aggtttaaca gcggattatc aggtcattaa gcaaatataa cgccctgaga atttcgacag
    33121 gcaaaagaaa aaggggttag catttagcta accccttatc ttatttggcg gaagcgcaga
    33181 gattcgaact ctggaaccct ttcgggtcgc cggttttcaa gaccgaagaa aaacaaataa
    33241 aaatcagtgc aatacgtgaa attgctgaaa agttaatcaa atttaacacc aagaaaaatc
    33301 aaggtattag gaagttgctc atagctagta taccaagtct ttttgccaga aatttttctg
    33361 gattgtctac ttgctaacca cgagcggcaa atccagtgtt ggtgtgattt ttgttttacg
    33421 atcgtaaatc aacacctgat tttctgtttt atgcccgctg aaaatttgtt tgtcgcgact
    33481 gctgccttcg taatctgaga tccccttggc ttttatgtcg tgaaaattgc acccgaacgg
    33541 aacgccaact ttttgctcgg ctgcgcgttt agcctgattc caccagttat tcagcgtttt
    33601 agctatgact ttcccgcctt tggttgtatt gatcacatat tcgcatgtac aggaagatac
    33661 atttcgggct aactggatcg ctgtacgtaa tcgcggtgac cattccttga tttgtttggt
    33721 gccggttttg ttttgctcaa tgtcccttta tccataatat cctgccattt caactcaagc
    33781 acatcaccga gtcttgccgc acagagatag gaaatctcca ttgcaatgcg taactgtgga
    33841 attgcttccg catatatcgc cgcatactgt tcatcggtga tgtaaacagt gcgggcttta
    33901 agagagaatt ttctgactcc tttgcatggg ttattcttca catacccacg ctcatatccc
    33961 cacccgtata ctcgactcag gcttgccagt tcatgatttg cctgggtctt gctctcaagc
    34021 cctcgtttat ccatgaaaat tcttacctgc tcagttttga cattatcagc aagcactttt
    34081 ccgaatacca tcagcaacgc cttctgatgt tgtcgataat ctttttgggt tcggggggcc
    34141 agttctgtaa atgcagggga gtccataaac atgtgccata atttagctac ggtcattatg
    34201 ttgtggagtt ttgctttttc cagttcataa ttttgccaga ctttagctac gctggttttc
    34261 cgcactcttc ctagccctat agttcttgta cttccttctg gtttccacac gtaactgtaa
    34321 ccattcgatc taacccgcgg tggcagtaca ttatcttttt tattttttct tggtcttccc
    34381 attattcagc gcctcaaaat cgggttcagc agaaaccagt tcagatgttt ttggcatcgt
    34441 tgtcagtccg tgtggaatat ccctgcgtag aactattggt tcgtttttag gaccgattac
    34501 aaatgggatg ccgtgcagtc ttaactggtg ttgctgtttt gtgtatcgct cgtatttcgt
    34561 gatctcttta atctctgctg gagatagagt taattcgtac atgtggtcac gtgcctttac
    34621 agcatgaccg ccgccactgt agctggtggg cggcgatatg ggttgaacat taaaaatcag
    34681 cctgactcgg gatcagtttt tgccagatag ctgaaacgta ttttgcctga taacgggcgt
    34741 catcaagtgc attatggcgc tcaccttcga atgggatagc
//
LOCUS       DAZUFB010000044        34368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZUFB010000044 DAZUFB010000000
VERSION     DAZUFB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.044"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..700
                     /locus_tag="ACHY3M_002378"
     CDS             <1..700
                     /locus_tag="ACHY3M_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFS4317284.1"
                     /translation="WFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFD
                     DAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVN
                     QIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQ
                     LTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRG
                     MAKQIARLAEQSTD"
     gene            799..1812
                     /gene="ybjS"
                     /locus_tag="ACHY3M_002379"
     CDS             799..1812
                     /gene="ybjS"
                     /locus_tag="ACHY3M_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4317285.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1809..2639)
                     /gene="amiD"
                     /locus_tag="ACHY3M_002380"
     CDS             complement(1809..2639)
                     /gene="amiD"
                     /locus_tag="ACHY3M_002380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFS4317286.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLVKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2636..2959)
                     /gene="ybjQ"
                     /locus_tag="ACHY3M_002381"
     CDS             complement(2636..2959)
                     /gene="ybjQ"
                     /locus_tag="ACHY3M_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4317287.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3085..3600
                     /gene="ybjP"
                     /locus_tag="ACHY3M_002382"
     CDS             3085..3600
                     /gene="ybjP"
                     /locus_tag="ACHY3M_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4317288.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3818..4546
                     /gene="artP"
                     /locus_tag="ACHY3M_002383"
     CDS             3818..4546
                     /gene="artP"
                     /locus_tag="ACHY3M_002383"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFS4317289.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4564..5295
                     /gene="artI"
                     /locus_tag="ACHY3M_002384"
     CDS             4564..5295
                     /gene="artI"
                     /locus_tag="ACHY3M_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFS4317290.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5302..6018
                     /gene="artQ"
                     /locus_tag="ACHY3M_002385"
     CDS             5302..6018
                     /gene="artQ"
                     /locus_tag="ACHY3M_002385"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFS4317291.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6018..6686
                     /gene="artM"
                     /locus_tag="ACHY3M_002386"
     CDS             6018..6686
                     /gene="artM"
                     /locus_tag="ACHY3M_002386"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFS4317292.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6979..7710
                     /gene="artJ"
                     /locus_tag="ACHY3M_002387"
     CDS             6979..7710
                     /gene="artJ"
                     /locus_tag="ACHY3M_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFS4317293.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7885..9012)
                     /gene="rlmC"
                     /locus_tag="ACHY3M_002388"
     CDS             complement(7885..9012)
                     /gene="rlmC"
                     /locus_tag="ACHY3M_002388"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFS4317294.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(9053..9541)
                     /gene="ybjO"
                     /locus_tag="ACHY3M_002389"
     CDS             complement(9053..9541)
                     /gene="ybjO"
                     /locus_tag="ACHY3M_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFS4317295.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9601..10446)
                     /gene="potI"
                     /locus_tag="ACHY3M_002390"
     CDS             complement(9601..10446)
                     /gene="potI"
                     /locus_tag="ACHY3M_002390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFS4317296.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(10443..11396)
                     /gene="potH"
                     /locus_tag="ACHY3M_002391"
     CDS             complement(10443..11396)
                     /gene="potH"
                     /locus_tag="ACHY3M_002391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFS4317297.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11406..12539)
                     /gene="potG"
                     /locus_tag="ACHY3M_002392"
     CDS             complement(11406..12539)
                     /gene="potG"
                     /locus_tag="ACHY3M_002392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFS4317298.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12634..13746)
                     /gene="potF"
                     /locus_tag="ACHY3M_002393"
     CDS             complement(12634..13746)
                     /gene="potF"
                     /locus_tag="ACHY3M_002393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFS4317299.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVGAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14097..14573)
                     /gene="ybjN"
                     /locus_tag="ACHY3M_002394"
     CDS             complement(14097..14573)
                     /gene="ybjN"
                     /locus_tag="ACHY3M_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFS4317300.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQILMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14661..15563)
                     /gene="rimK"
                     /locus_tag="ACHY3M_002395"
     CDS             complement(14661..15563)
                     /gene="rimK"
                     /locus_tag="ACHY3M_002395"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFS4317301.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15624..16346)
                     /gene="nfsA"
                     /locus_tag="ACHY3M_002396"
     CDS             complement(15624..16346)
                     /gene="nfsA"
                     /locus_tag="ACHY3M_002396"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFS4317302.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16330..16617)
                     /gene="ybjC"
                     /locus_tag="ACHY3M_002397"
     CDS             complement(16330..16617)
                     /gene="ybjC"
                     /locus_tag="ACHY3M_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFS4317303.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16777..17034
                     /gene="grxA"
                     /locus_tag="ACHY3M_002398"
     CDS             16777..17034
                     /gene="grxA"
                     /locus_tag="ACHY3M_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFS4317304.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(17064..17441)
                     /gene="ybjM"
                     /locus_tag="ACHY3M_002399"
     CDS             complement(17064..17441)
                     /gene="ybjM"
                     /locus_tag="ACHY3M_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFS4317305.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17711..19396
                     /gene="ybjL"
                     /locus_tag="ACHY3M_002400"
     CDS             17711..19396
                     /gene="ybjL"
                     /locus_tag="ACHY3M_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFS4317306.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19571..20107)
                     /gene="rcdA"
                     /locus_tag="ACHY3M_002401"
     CDS             complement(19571..20107)
                     /gene="rcdA"
                     /locus_tag="ACHY3M_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFS4317307.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            20191..21399
                     /gene="ybjJ"
                     /locus_tag="ACHY3M_002402"
                     /pseudo
     CDS             20191..21399
                     /gene="ybjJ"
                     /locus_tag="ACHY3M_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            21399..22214
                     /gene="ybjI"
                     /locus_tag="ACHY3M_002403"
     CDS             21399..22214
                     /gene="ybjI"
                     /locus_tag="ACHY3M_002403"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFS4317308.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            22300..22584
                     /gene="ybjH"
                     /locus_tag="ACHY3M_002404"
     CDS             22300..22584
                     /gene="ybjH"
                     /locus_tag="ACHY3M_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFS4317309.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22625..23857)
                     /gene="mdfA"
                     /locus_tag="ACHY3M_002405"
     CDS             complement(22625..23857)
                     /gene="mdfA"
                     /locus_tag="ACHY3M_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFS4317310.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            24142..24738
                     /gene="ybjG"
                     /locus_tag="ACHY3M_002406"
     CDS             24142..24738
                     /gene="ybjG"
                     /locus_tag="ACHY3M_002406"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4317311.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24796..25554
                     /gene="deoR"
                     /locus_tag="ACHY3M_002407"
     CDS             24796..25554
                     /gene="deoR"
                     /locus_tag="ACHY3M_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFS4317312.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(25601..26803)
                     /gene="dacC"
                     /locus_tag="ACHY3M_002408"
     CDS             complement(25601..26803)
                     /gene="dacC"
                     /locus_tag="ACHY3M_002408"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS4317313.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            27050..27676
                     /gene="gstB"
                     /locus_tag="ACHY3M_002409"
     CDS             27050..27676
                     /gene="gstB"
                     /locus_tag="ACHY3M_002409"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFS4317314.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27673..28788)
                     /gene="yliI"
                     /locus_tag="ACHY3M_002410"
     CDS             complement(27673..28788)
                     /gene="yliI"
                     /locus_tag="ACHY3M_002410"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFS4317315.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28899..29282)
                     /gene="bssR"
                     /locus_tag="ACHY3M_002411"
     CDS             complement(28899..29282)
                     /gene="bssR"
                     /locus_tag="ACHY3M_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFS4317316.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29495..30820
                     /gene="rimO"
                     /locus_tag="ACHY3M_002412"
     CDS             29495..30820
                     /gene="rimO"
                     /locus_tag="ACHY3M_002412"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFS4317317.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(30867..32195)
                     /gene="dgcI"
                     /locus_tag="ACHY3M_002413"
     CDS             complement(30867..32195)
                     /gene="dgcI"
                     /locus_tag="ACHY3M_002413"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4317318.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32203..>34368)
                     /gene="pdeI"
                     /locus_tag="ACHY3M_002414"
     CDS             complement(32203..>34368)
                     /gene="pdeI"
                     /locus_tag="ACHY3M_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4317319.1"
                     /translation="ALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYY
                     LFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNC
                     FDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHE
                     IIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNA
                     SDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIRE
                     GDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGI
                     IKLCGLEAVEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         8607 a         8898 c         8382 g         8481 t
ORIGIN      
        1 gtggtttctc aatgtgatta cttccgtacc gcccaccacc gccagggcgt tgattcaggg
       61 gctgaaacac gatctgctgg cggatgatac cgcgctacgt gcactcatcc cacaacggct
      121 gatcgctttc gatgacgcgg tacgtagcac gttgaaagag gaggaaaaac tggtcaactc
      181 cagcgactgg ggctacgacg ctcaggcctt tgcccgctgg cgaccagagt acggttattt
      241 tgccaaacag gcggggttta ccgttaaaac gtccgccagc cttgctgctt tatggcaggt
      301 agtgaaccaa atcggcggta aagagcgtta tttctttggc aatattttgt ggcagacacg
      361 ggcgttgatg gaccgcgcga tcggtcataa gctggcgaaa ggccgcccgg agcgcgaata
      421 tttacagact ggcgatgcgg tggatagctg gaaagtgatt gtcgttgaac cggaaaaaca
      481 acttacgttg ttatttggca tgaaagcgcc ggggctggga cgactgtgtt ttagcctgga
      541 agataaaggc gactatcgta ctatcgatgt ccgcgctttc tggcatccgc acggtatgcc
      601 ggggctgttt tactggttat tgatgatccc cgcgcatctg tttatttttc gcggaatggc
      661 aaaacaaatc gccagactgg cagaacaaag cacagattaa tcgacaaaac ctgcgttatt
      721 ctttcatgtt tcccattgca tcatggggga aatcacggaa gaatgcgcac gttattttct
      781 caacaacagt ggattgatat gaaggtactg gttaccggcg ccaccagcgg cttaggtcga
      841 aacgcggtag agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg tcgcaacgag
      901 gcaatgggca aattgctgga aaaaatgggc gcagagtttg ttccggcgga tctgaccgag
      961 ctggtttcat cacaagctaa agtgatgctc gcgggcattg atacgctgtg gcactgctcc
     1021 agctttacct caccctgggg gacacaacag gctttcgatc tggctaacgt tcgcgccact
     1081 cgccgcctgg gtgaatggac tgtcgcctgg ggtgtgcgta actttattca tatctcttcc
     1141 ccctccctgt acttcgatta tcaccaccat cgcgatatta aagaagattt tcgccctcac
     1201 cgcttcgcca acgagtttgc ccgcagcaaa gcagccagcg aagaagtgat caatatgctt
     1261 tcgcaggcga atccacaaac gcgctttact attctgcgcc cacaaagtct gttcggaccg
     1321 cacgataaag tctttattcc ccgtctggcg catatgatgc accactacgg cagtattctg
     1381 ttaccgcatg gcggcagtgc gctggtcgat atgacctact atgaaaatgc cgtgcacgca
     1441 atgtggctgg caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatc
     1501 accaacggcg agcatcgcac actgcgcagc atcgtgcaga agctgatcga cgagttgaat
     1561 attgactgtc gtattcgttc cgtcccctac ccgatgctgg atatgatcgc ccgcagcatg
     1621 gagcgtttag gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctccaag
     1681 ttgaattttg actttacgct ggatattacg cgtgcgcagg aagagttagg ttatcagccg
     1741 gtcatcaccc tggatgaagg tatcgagaaa actgccgcct ggctacgcga ccacggaaaa
     1801 ctgccgcgct aatcctgccc gtatttctcc agcaatgctt cggcaatcgc ctgagtttct
     1861 gcatccgctt cgccgttata taacgtcggg cggaaatgca tctggaatgc cataatcacg
     1921 cgccgctgct cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc
     1981 tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag gtaaaagtta
     2041 acccgctgcg catccggcca ggcaccaatc ccctgctgcg ccagttgctg ccagggaaat
     2101 aatggccccg gatcgtcttt gcgctgcggt gcgatatccg catgtgccac tacgttttcc
     2161 ggcttgatgt gataacgggc aataatatct ttcaccagtg gaataagcgc ctgaatctgt
     2221 gccggttcaa acggggcaaa atatttcact ccggccgatt tttgccagcc acggttttcc
     2281 agctcaatgc caatagaggt gtcgttaagg cgcgttgccc cgcgccaggc gctaatcccc
     2341 gcatgccagg ccagttcttg ttccggcacc agttgccaga tgcgcggttt accgttgtat
     2401 cgcggtggta ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc
     2461 agcgagctat caaaatcatc tgcggtgtag tggatcacca gcactttaat gcgcggatac
     2521 gccgcctgcg cctggcgtcg ggtatcaagc tgatatccct ctttctcgac aatgcctttt
     2581 tcgcctgcac accctgccag taacagagcg gcagcgatca gccagaagac tcttctcatc
     2641 gacgcgtttt caccgccgta ccgctgacgc taaccatcag catactgccg ttttgcccga
     2701 ccgtttcgta gtcgatatca ataccgacga cggcatcggc ccccagcgcc cgcgcctggg
     2761 agcctaattc ctcaaaggcg atctcccgtg ctttacgcag ttctttttca tacgcaccgg
     2821 aacgtccgcc aacgatatcg cggataccgg caaagaaatc acggaaaata ttggcaccta
     2881 aaatcgcttc gccggtcacc acaccgcaat attcaacgat gatctggcct tccagagttg
     2941 gggttgtaga aaattgcatg atcgatctcc ttgtttgtta agccttcgat ctctaaagca
     3001 ttatcagact gatacgctat tattgaaagg atatcattat tatcgtagaa cgctttcaga
     3061 gcgatcgctt ataaggaaat cattatgcgc tacagcaaat tgacaatgct tatcccctgc
     3121 gcactgctcc tcagtgcctg caccacagtc actccagctt ataaagataa cggcacacgc
     3181 agtggtcctt gcgttgaagg aggcccggat aacgtggccc agcaatttta tgactaccgc
     3241 attttgcacc gcagcaacga catcaccgcc ttgcgccctt atctgagcga caaactggcg
     3301 acactgctta gcgatgcctc ccgggataat aaccatcggg aactactgac caacgatcca
     3361 ttctccagtc gcacaacctt gccagagagc gcccgtgttg ccagtgcctc aacaatcccg
     3421 aatcgtgatg cgcgaaatat tcctctgcgt gtggatctaa aacagggtga tcagagctgg
     3481 caggatgaag tgttaatgat tcaggaaggc cagtgttggg tgatcgacga tgtacgctat
     3541 ttaggcggca gcgttcatgc cacggcgggt acgctccgtc agtccatcga gaatcgctaa
     3601 cgccttgatc aaccaataaa ccccgtaaat tgtccggcat ttcgtgagga atgccgacat
     3661 ttatgctcgc cgaccaccgc ccccgttatt ttgtgctatg tttattgaat aatgcgcttt
     3721 gcttttaact tttaaagcag aaatattgca taattattct gtcaaaggta ctatcttcga
     3781 cctcaatagc tatcagactg ccagtatacg agtgtcaatg agtattcaat taaacggcat
     3841 aaattgcttc tacggcgcgc atcaggcgct gttcgatatc acgctggatt gcccacaggg
     3901 cgaaacgctg gtgttacttg gccccagcgg cgcgggtaaa agctcgctgc tgcgtgtact
     3961 caatctgctt gagatgccgc gctccggtac gctcaacatt gcaggcaacc atttcgattt
     4021 caccaaaaca ccctctgaca aagcgattcg cgatttgcgt cgtaacgttg gcatggtgtt
     4081 tcagcaatac aacctgtggc cgcatctgac cgtgcagcaa aacctgattg aagcgccctg
     4141 ccgtgtactg gggttgagta aagatcaggc gctggcccgt gcagaaaaac tgctggaacg
     4201 tctgcgtctc aaaccttata gcgatcgtta cccgctgcat ctttctggtg gtcagcagca
     4261 gcgtgttgct attgcccgtg cgttgatgat ggaaccgcag gtactgctgt tcgatgaacc
     4321 gaccgccgca ctggacccgg aaattacggc acaaatcgtc agcatcattc gtgagctggc
     4381 agaaacgaat attacccagg tgatcgtcac ccacgaagtt gaagtggcgc gtaaaaccgc
     4441 cagccgagtg gtgtatatgg aaaatggtca tatcgtagaa caaggcgacg cgagctgctt
     4501 taccgagccg caaaccgaag catttaaaaa ctatctctct cactaagatt cgggataaca
     4561 acaatgaaaa aagttctgat tgccgcgtta attgcaggtt ttagtctttc cgccacagct
     4621 gccgaaacca ttcgttttgc taccgaagca tcctatcctc cgtttgaatc gattgatgca
     4681 aacaaccaga tcgttggttt tgacgtcgac ctggcacaag cgctgtgtaa agagattgat
     4741 gcaacctgca ctttctctaa ccaggcgttt gacagcctga tcccaagcct gaaattccgt
     4801 cgcgtagaag ccgtgatggc gggcatggat atcactccgg agcgtgaaaa gcaggtgctg
     4861 tttaccaccc cgtactatga caactctgcc ctgtttgtgg gtcagcaagg caaatacacc
     4921 agtgttgatc agctgaaagg caaaaaagtc ggcgtacaga acgggacgac acaccagaaa
     4981 ttcattatgg ataagcaccc ggaaatcact accgttccgt atgacagcta ccagaacgca
     5041 aaactggatc tgcaaaacgg gcgtatcgac ggcgtcttcg gtgacaccgc agtggtcact
     5101 gagtggctga aagataaccc gaaactggcg gcggtgggcg acaaagtgac cgataaagat
     5161 tacttcggca ctggcctcgg catcgcggta cgtcagggca acactgagct gcagcagaaa
     5221 ctcaacactg cgctggaaaa agtgaagaaa gatggcactt acgaaaccat ctacaacaaa
     5281 tggttccaga agtaattcct gatgaatgaa ttttttcctt tagcaagcgc cgccgggatg
     5341 accgtcggcc ttgccgtttg tgcattgatt gtcgggctgg cgctggcgat gttctttgcg
     5401 gtatgggagt cggcaaaatg gcgtcctgtc gcgtgggcag gttcagcgct ggtaaccatt
     5461 ctgcgtggcc tgccagaaat tctggtggtg ctgtttatct attttggctc ctcgcagctg
     5521 ctgctgacgc tttcggatgg cttcactatc aatcttgggt tcgtgcagat cccagtgcag
     5581 atggacattg agaacttcga cgttagcccg ttcctttgtg gtgtcatcgc tctgtcactg
     5641 ctgtatgccg cctatgcctc gcaaacgctt cggggcgcgt tgaaagcggt gccggtgggt
     5701 cagtgggagt ctggtcaggc gctggggctg tcgaaatcgg ctatcttttt ccgtctggtg
     5761 atgccgcaga tgtggcgtca tgcgctgcct ggcctcggta accagtggct ggtgctgctg
     5821 aaagataccg cgctggtcag tttgattagt gtgaatgatt taatgctgca aacaaaaagc
     5881 atcgctactc gtacccagga accatttacc tggtacattg tggcggcggc gatttacctg
     5941 gtgatcaccc tgctcagtca gtacattctc aaacgcattg acctgcgcgc gacacgtttt
     6001 gagcggaggc ccagctaatg tttgagtatt tacccgaact gatgaaaggg ctacacacca
     6061 gcctgacgct aaccgttgcc tcgctgattg tggcactgat tctggcattg atttttacca
     6121 tcatcctgac gctgaaaacg ccggtgctgg tgtggctggt gcggggttat atcacgctgt
     6181 ttaccggtac gccgctgctg gtgcagatct tcctgattta ttacgggccg ggccagtttc
     6241 cgactttgca ggagtatccg gcactgtggc atttgttgtc agaaccgtgg ttatgtgcgc
     6301 tgattgcgtt atcgctgaac agtgcggcgt ataccacgca gctgttttac ggtgcaattc
     6361 gtgcgatccc agaaggtcag tggcagtcct gtagcgccct gggaatgagc aaaaaagata
     6421 cgctggcgat cctgctgccg tatgccttta aacgctcgct ctcttcttat tccaacgaag
     6481 tggtgctggt gttcaaaagt acctctctgg cttacaccat tacgctgatg gaagtgatgg
     6541 gatacagcca gttgttgtac ggacgcacct acgatgtaat ggtgttcggt gcggcaggga
     6601 ttatttacct ggtcgtcaac ggcctgctga cgctgatgat gcgtctgatc gagcgcaaag
     6661 cactggcatt cgaacggcga aattaatgta gctgcgtaca ttcgccgggg ggggtttgta
     6721 ggtctgataa gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg
     6781 gcgtggacgc cttatccggc ctacatagtg cataaattct tataatgaag acggacaact
     6841 cactaaggta tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat
     6901 aaattcactt gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg
     6961 acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact
     7021 ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt
     7081 gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg
     7141 tgcaaacaaa tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg
     7201 tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac gcccgagcgt
     7261 agcaagcagg tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa
     7321 aaagatacct acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc
     7381 accacgcacc agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac
     7441 agttatcaga atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac
     7501 acagcggtgg taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa
     7561 gtgaccgatc cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa
     7621 gctctgctgg aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag
     7681 aaaatcaatg accagtggtt tccgcagtaa ttgagactga tgacaaaagt aagattgcct
     7741 gatgcgctgc gcttatccgg cccacatcaa ctctgcaatg tattgattta gcatgatttt
     7801 gtaggccgga taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa
     7861 tctgaagccg cacctgcggc ttttttattg cttcaccagc agcgtcagca cttcatagtg
     7921 cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc
     7981 gcggatatct ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc
     8041 cattgttgag agataatcac atagcggttt accaatgccg cggcgcggcg gattaaccag
     8101 caccagctcc ggcacctccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa
     8161 ttgcaaacgc gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga
     8221 tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc
     8281 cacaccgcag aacagatccc acatatgttt aactggcagc tgccgtaccc agtcgcgcgc
     8341 ggtagcgtac agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat
     8401 ccacagcggt acgtcattaa aacgctctgc cagcgcctgt tgttcagtaa ggtagatctc
     8461 cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag
     8521 ttgcggtagt tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc
     8581 agaacgcagt acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat
     8641 gtatttcagt tcgccacgct tacgcgccac gttgtagggt gtcagccccg cacgggcgat
     8701 aaacggtttt agcgccgcaa aaacgggcgc aaatgaggca ggataaagcg ggcagtcaca
     8761 aaggtcttct ggtgtgccgt cgcgatgcag cataccgagc agtggttttt caacgctacc
     8821 actcaccacc attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca
     8881 ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag
     8941 ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag
     9001 tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca cattattgca
     9061 actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg
     9121 gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga
     9181 acagctccgg ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt
     9241 gtgtcagcag atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc
     9301 agatctcaat gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt
     9361 gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca
     9421 gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc agcgcaggca
     9481 cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca
     9541 tgaaaacaat gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct
     9601 tcagccatgt cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag
     9661 ataccaggcg ataaatccga caattccgac cgcaccgaga attaacgttg caagggcgtt
     9721 gatttccgga ttcaccccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc
     9781 tcccggcccg gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag
     9841 taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt
     9901 cagcggcgtc gcaccgagat ccatcgctgc ttcttctatc gagcgatcca gttcccgcaa
     9961 tcttgaagaa ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat
    10021 ggtgagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa
    10081 caacagcgac aagcccgtga tgacatctgg catcaccagc ggcgcggtga tcataaaggc
    10141 aaagccattt gatccgcgaa acctgccaaa gcgcaccagc accaccgccg caatagtccc
    10201 gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact
    10261 catcatcgcg tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac
    10321 cgtcaccagc ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag
    10381 aaaggtaaag cccagcagca aaatcacaat ccgccagggc gaacgaacta ccggtaaatt
    10441 attcatccgt gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca
    10501 attagcagca gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg
    10561 aaaaactctt gccatagcac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc
    10621 gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc aataatccca
    10681 cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc
    10741 agcgctgctt ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc
    10801 ataaacggca cgtaggcgta aacaatgcca atataaactg ccagattggt atgaagaatg
    10861 gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt
    10921 tttaatattc ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc
    10981 accagtagta ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc
    11041 ggatagccga tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga
    11101 taagcatcga aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg
    11161 atggacagtt gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc
    11221 atctccgcca ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac
    11281 cagatatatg gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac
    11341 cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt attcatcttt
    11401 gctccttaaa ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg
    11461 ccccaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctaatcatc
    11521 tgcccacttt tcagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc
    11581 acctccccca ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt
    11641 tccgggcgca gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact
    11701 ttcagtggat gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg
    11761 agtacacctt caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga
    11821 tgctcgtaga tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt cataatggcg
    11881 atacgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca
    11941 ccgacgcgct ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc
    12001 agcgcgccca tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc
    12061 agggccacac gctgtcgctg accaccggaa agctgatgcg gtttgcgttt ggcgaactcc
    12121 tgcatgtgca ccagcccaag catctcgttg acccgggtgg caatttccgc tttcggtagt
    12181 ttgtcctgtt tcaggccaaa ggcgatgttc tgctccacag tcatatgggg aaacagcgcg
    12241 taagactgaa acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg
    12301 ccatcaagca ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc
    12361 gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc
    12421 aggctgacat catccaccgc atgttgacca tcgtaggatt tggtgaggtt gcggatttct
    12481 aatagcggcg tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg
    12541 gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggcgggt
    12601 gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca
    12661 cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact ttcagagtga acagcttcgc
    12721 acgaacatcc gcaggcggat aaatacccgg gttctcacgg acttccgcac ccaccagcgg
    12781 cgtggctgct ttattggcgt tggcatagaa cacattgtcg gaaatatgcg ctactacatc
    12841 cgggcgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg catccgcagg
    12901 catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga acgagacatt
    12961 cactccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac ctgcccagcc
    13021 gatagcgacg caaatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata
    13081 acgaatgttc gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatccgc
    13141 tttagtgctg ttgggatctt tgccgagata attcaacacg gtagcaaaaa cttcttctgg
    13201 cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag gtttgaggat
    13261 caagtcccag ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt
    13321 atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg
    13381 tttggcgacc agcttcagca gctccgggtc gagattcttc cactccggca atttgctttt
    13441 gtccagcggc tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg cagatggaac
    13501 caccaagtca aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc
    13561 gaaaacatcg tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg
    13621 ggcgatataa tcagaccagt tataaacgtg gagtgttttt tgttcagccg cgagcgtgcc
    13681 gacagagacg gccatcagag cacccgcagc cagacccgaa agccattttt tatttaaggc
    13741 ggtcatatct ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactaaa
    13801 agatatgcaa taaacgtgca tattttttat cggcggggtg taagaaaaga gaactctctc
    13861 ccggccagca ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag
    13921 actatagctc ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg
    13981 ataaagccaa atcgacacaa gagaagtgaa ataatcttta agaaaaggtt aaaaacagca
    14041 gataattttg cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag gagttatcag
    14101 tggaggaaat agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt
    14161 tgatgggcat tagcttcaag aatgaccatc aaaatctgct cttcgctctg acgcacgaac
    14221 catgcaaact gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc
    14281 tttggcagat tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg
    14341 atggcagata aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca
    14401 gaaaacagga tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc
    14461 tgcatatggg gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg
    14521 tcatcgagcc attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg
    14581 tacaacctct ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat
    14641 gatcagtaat gtgattgcga ctaaccaccc gttttcaggc aatattctgt cgtagcgtgg
    14701 cgttcgatcc agcggatcat tttacccgcg atgtcgatac cggtggtttt ttctattcct
    14761 tccagccccg gcgacgcatt cacctccatc accaacggcc cgcgattagc acgcagaata
    14821 tccacaccag caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc
    14881 tgtggtgtga tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg
    14941 ccctctttcg cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg
    15001 atatcgcacc cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg
    15061 cggaaagcgt caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt
    15121 ccctgcgtgc cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa
    15181 tcgctggtat catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc
    15241 gccagcagtt gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg
    15301 agcggatagc tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg
    15361 gcggtgccaa tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga
    15421 atagaagacg ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc
    15481 aggtgaccgc gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc
    15541 cgggacaata tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg
    15601 tatcatacat cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc
    15661 agaataaatg ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc
    15721 cggcgattat tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc
    15781 agtgcgcctt tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa
    15841 cgcggcttaa gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga
    15901 acatgctgcg gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg
    15961 atatataccc cgcccaatcc cagcgattcc gctgcgatta atgcattctg cgccatcatt
    16021 gccgtatcaa cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag
    16081 atctgtaaat ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg
    16141 tgtttttgcc cgccggtcag cgtcaccagt tcttcacgta acgctttgtc ggtaatgcga
    16201 ataatgctac tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc
    16261 gcctcacgct gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca
    16321 caaataagtt caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg
    16381 tggacggttc atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc
    16441 agcgggaagc atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga
    16501 aaatggctca ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat
    16561 cattccgata aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg
    16621 cttccctctg caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt
    16681 atagctttta gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt
    16741 gatcattaca ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt
    16801 tcgggttgcc cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc
    16861 gatgattttc agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta
    16921 caacaaaagg caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat
    16981 atcggcggct ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct
    17041 gacaagccct cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta
    17101 ctgataaata agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat
    17161 gccagctctt gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcattgaa
    17221 cagggtgccg ccagcattgc gccaaataga ggtttcagga cttctctacg ctgtgaaaag
    17281 aagctggcga ctgctccagg aagaatgaaa aatagcaagc cgatttcagg atgcccggca
    17341 gcccgaaaag cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca
    17401 aaacagcaga ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc
    17461 gtctatcgaa cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc
    17521 catgctaata taggccaacg caattcatat agccgttgat acctaatgtg attacactag
    17581 taaaatatat tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt
    17641 ctggtaaaaa acattatcgt aaattaccat ttctttcaac agtttactag taaacaagaa
    17701 gttagcctcc gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt
    17761 atttgtggtc ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact
    17821 gggtaattcc attggcgttt tagtcgtatc gctgttatta ggccaacaac atttcagcat
    17881 taacaccgat gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc
    17941 cggaccgaac tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact
    18001 ggtgatggtt ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga
    18061 tattggcctg acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg
    18121 tgctggcgat acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga
    18181 taatctgagc ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg
    18241 tgcgcgttac ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc
    18301 ccgcgaacgt ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc
    18361 ctatcgcgtc ggcccggagc tggtggcctg gaccgacggc aaaaatctgc gtgaactggg
    18421 tatttatcga caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa
    18481 tccagacggt gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga
    18541 cgcccatgcc cgactcgatc ccagcttccg taacggtaaa gaagttttcg atcgtgacct
    18601 tctcgacatg cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ctgtaggtaa
    18661 acgtctcgca caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag
    18721 ccagattgag atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt
    18781 cagcggtgat gcccgtcgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca
    18841 cagccaggtc actgacctgc tggcattttg cgccttcttt gttattgggc tgatgatcgg
    18901 gatgatcacc ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt
    18961 attcgccgga attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc
    19021 gcaaggtgca ttaagcatgg tgaaagagtt cggcttgatg gtgtttatgg caggcgttgg
    19081 tctgagcgcc ggtagcggta ttaataacgg cctgggcgcg attggcggtc agatgttgat
    19141 tgccggatta attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt
    19201 attgcgaatg aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc
    19261 ggcaatggag atcatcagtg atacagctcg cagtaacatc cctgcgctgg gctatgcggg
    19321 cacctacgca atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg
    19381 gccaggatta ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag
    19441 aacttttccg cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt
    19501 ttgtggagcc catcaacccc gccatttcgg ttcaaggttg atgggttttt tgttatctaa
    19561 aatttatcta ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg
    19621 gcttacggtc ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg
    19681 cggttccggg ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca
    19741 ttaccgtttt taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca
    19801 tgttatccgg cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc
    19861 cttgagcatc actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa
    19921 aactgctgaa cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc
    19981 ccaacggtac cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa
    20041 gtttcaccgc ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc
    20101 gacgcatgtc atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt
    20161 gtcttccctc ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg
    20221 aaacgccgaa cctgggcgct gtttatgttc ttctttttgc cgggcctgtt aatggcgtcc
    20281 tgggcaaccc gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc
    20341 ggtgttctct ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta
    20401 gtgaaacgct ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg
    20461 atgatgatat taagtctggc actcaggctg acatcacccc tgctcttcgc ggttggcctc
    20521 ggcgtctttg gggcaagttt tggttctgcg gaagtggcga taaacgtcga aggtgccgcc
    20581 gttgagcgag aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc
    20641 acgctggcag gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg
    20701 cacattttat tggcggcgct ggtaggaatc gcgcctattt atatcgccat tcaggcaatc
    20761 cctgacggta cgggcaaaaa tgctgccgat ggcacccagc atggtgaaaa aggcgtacct
    20821 ttttatcgcg atatccagtt gctgctgatt ggtgttgtgg tgctggcgat ggcctttgcc
    20881 gaaggttctg ccaacgacta gttaccctta ttaatggttg acggtcacgg ttttagccct
    20941 acttccggct cgctgattta tgccggtttt accctgggga tgactgttgg acgctttacc
    21001 ggcggttggt tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg
    21061 ggagcgttgg gtattgggct gattattttt gtcgatagcg cctgggtcgc tggggtgtct
    21121 gttgtactct ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc
    21181 gataccggcc ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct
    21241 ttcctcgtcg ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca
    21301 atgctggttg tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa
    21361 cccgatacca aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg
    21421 tagacatgga tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg
    21481 ctcagtatca gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat
    21541 attatcagtt gatctctttc ttccctgaaa ttgcgaatga aattgccttt gtggctgaaa
    21601 acggcggctg ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg
    21661 cgtttgctac tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg
    21721 gaaaaaatag cgcctataca ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa
    21781 tgtattatca ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta
    21841 agtttggcct gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg
    21901 ccatcggcga tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc
    21961 ccggcgtaca taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca
    22021 gcgaagtggt ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct
    22081 ttagttttgc aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgcgcag
    22141 gctccaataa ccgtgaaggc gtactggatg tgatcgataa agttcttaaa cacgaagcgc
    22201 catttgacca ataaaaacgg cagcacgccc ggcaacacaa tgcctgaatg aaggctgcat
    22261 aattcgcgga aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta
    22321 ctgttgggcg cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc
    22381 gatgtgccga gtggatacaa agtggttgtg gttcctgatt cagtcagcgt tccacaagcg
    22441 gtcagcgtcg cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac
    22501 cgcccgcatc cttatgttcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac
    22561 cagattgacg accatcacca ttaattcgag aatgcctgat cgcacaaagc aatcaggcat
    22621 ttttttaccc ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg
    22681 acagccacaa aattccgttg acaaggttga agagattaaa cagtccgttg cccccgttca
    22741 gccaggcatg tttgctgatt tcaataccga cggtaaagat cagcatttgc agcattccca
    22801 tcgccgcaga aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc
    22861 ccgcattcgc cagaccaatg ccgaaagcat aaatacttaa cccggcggtc atccataaat
    22921 acgcgtgcga tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc
    22981 agccgcccat aattatcagc gaacgtacgg tgcggcgcga ggtcaaacgc gctaacaaca
    23041 agttacccgc aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc
    23101 tcaactgctc gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca
    23161 ggctaacgaa tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct
    23221 tatagtcacg accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag
    23281 gcatggcttg ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aagacaaaca
    23341 tcccttccca gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag
    23401 caatcagcgc cacgttcgcc atcagcgcgg tgatcttgat gcaaaccgcc tcttcgaagg
    23461 attcctgaat tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga
    23521 agcgcaacag agtgaattgt tcaatgtttt gcgccagtaa tattgccaga caggtgatga
    23581 taaaccacac cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc
    23641 ccaggagcca ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc
    23701 aatcaatgcc cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt
    23761 tgccgatata ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac
    23821 gtccaagcct ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat
    23881 cgcgcagagt ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg
    23941 gttaagcaca ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac
    24001 ctatgcccgt tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg
    24061 ctttacccag tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta
    24121 atcactttgc gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt
    24181 aacgcgacgc cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat
    24241 ttgattaccg tggtaccgct gctggccgcg gtgctttggt tgtgggggct tacagcacaa
    24301 cggcaactgg tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg
    24361 atgggacatc tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg
    24421 catcatgcgg cggatgactc attcccaagc gaccacggta cggtgatttt cacctttgca
    24481 ctggcatttt tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc
    24541 gtcattgcct ggtcgcgcgt ttatcttggc gtgcactggc cgctggatat gctcggtgga
    24601 ttgctggcag gtatgattgg ctgccttagc gctcagatta tctggcaagc gatggggcat
    24661 aaactctatc aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa
    24721 ggctgggtgc gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa
    24781 ctattcagag ggattatgga aacacgtcgc gaagagcgta tcgggcagct gctgcaagaa
    24841 ttaaaacgca gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag
    24901 atgacgattc gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat
    24961 attgttctgg aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg
    25021 gtggaagaaa aacgccgggc ggcaaaaatg gctgcgacac tggtagaacc cgatcagact
    25081 ctcttttttg actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc
    25141 ccttttaccg ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat
    25201 tgccgcgcat ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaacccatc
    25261 gattttcagc aaacgttgaa taatttttgc ccggatatcg ctttttattc cgcggcgggc
    25321 gtgcatgtca gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg
    25381 gccatgtcga tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg
    25441 cgtccggcgc gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg
    25501 gaagatgagt atgtgaagta cgcgcagacg caccgtatta agttgatgta ttaatgacgt
    25561 ataaccggat gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga
    25621 accactgatg gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt
    25681 ccacattttc catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat
    25741 caatggtccc gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg
    25801 tgtagctcgc cttcaggttt ttcagctgcc cacgcggtat ggtcactgag cccgcttcgc
    25861 ctgccccaag attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg
    25921 catcaggttt aattggcgtc acggtttcaa agaagcggaa accccaggtc aataatttct
    25981 cagactcatt aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca
    26041 tatcgccctg ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg gttcctgtct
    26101 tcatgccatc aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa
    26161 ttttgttgaa ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga
    26221 tcaatgcttt acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca
    26281 gaccgtgcac cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca
    26341 tcaaaccaat gaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt
    26401 cattaccgga ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg
    26461 gtttgaggaa cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt
    26521 taccgaccgt caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca
    26581 catagctggt catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt
    26641 ctgccagcac tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg
    26701 gcgcttcaac ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag
    26761 aacccgctgc aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat
    26821 ccattcaaaa aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac
    26881 acaaacttaa agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc
    26941 ttcgctgctt ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac
    27001 catcggagtc ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct
    27061 gtggggtcgg aataattcaa ccaacgtaaa aaaagtattg ctgacgctcg aagaactgga
    27121 actaccttat gagcaaattc tcgcgggccg tgagtttggg ataaatcacg atgctgattt
    27181 tctggcgatg aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat
    27241 tctttgggaa tcaaacgcca ttgtccgcta tctggcggca cagtacgggc aaaaacgtct
    27301 gtggatcgac tcaccggcac gtcgtgcgga agcggaaaaa tggatggact gggcaaacca
    27361 gacgctcagt aatgctcatc gcgggatcct gatgggatta gtcagaacac caccggaaga
    27421 gcgcgatcag gccgccattg atgccagttg caaagagtgc gacgccctgt ttgccctgct
    27481 cgatgcggaa ctggcaaaag taaaatggtt ctctggcgac gagtttggtg tgggcgatat
    27541 cgctatcgca ccgttcatct acaatttgtt taacgttggc ctgacctgga caccgcgtcc
    27601 aaatctgcaa cgctggtatc agcaactcac tgaacgcccc gcagtgcgca aagtggtgat
    27661 gattcccgtt agctaattgc gtgggctaac tttaagtaat tccccactgg actcgtcggt
    27721 gagaacgtat aaataaccgt cgggtccagt gcgaacatca cgaattcgct gccctctgtc
    27781 cgttaaaata cggccatctt ctgtcacttt gtcgccgttg acgctcatca caatgacatc
    27841 tttatctttc agcgcgccaa taaataattt ttgctgccac tgggggaatt tatcgctgtt
    27901 atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt
    27961 cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca
    28021 ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc
    28081 gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg ccattccttg
    28141 cggattacga atgccataag accagatctc ggcgcgcaca ccggattcct ttataaaagg
    28201 attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc
    28261 cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt
    28321 accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg
    28381 gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac
    28441 agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga
    28501 ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac
    28561 gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt gacgcaactc
    28621 gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg
    28681 atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc
    28741 ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctccgga
    28801 ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact
    28861 taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg
    28921 tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg
    28981 gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct
    29041 gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga
    29101 ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga
    29161 tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcagcg
    29221 aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac
    29281 atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct attttttgag
    29341 caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca tttccttcgc
    29401 ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc
    29461 ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa
    29521 aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac
    29581 cgaactacgc actgaaggtt atgacgtggt accgagctat gacgatgcgg acatggtgat
    29641 cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga
    29701 agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca
    29761 gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct atgagcaggt
    29821 tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt
    29881 gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa tttcggaagg
    29941 ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc tggtgagccg
    30001 tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat
    30061 tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc gtactggctt
    30121 ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact
    30181 ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc
    30241 actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc
    30301 gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa
    30361 acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc
    30421 tggcgagaca gaagaagatt tccagatgct actcgacttc ctgaaagaag cgcgtctgga
    30481 tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga
    30541 ccaggttccg gaagaagtta aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca
    30601 gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga
    30661 agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa tcgacggcgc
    30721 ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga
    30781 gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa
    30841 tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgagccct gccgtttttc
    30901 ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt ggtacattca
    30961 tttgaatgcg ctacaccgat actggtactt aaattaatgc ttcccacctg gaaatgaacg
    31021 atttctttta gaatcgatag caactcttcc agcgttccac ccgtataaat caccgcaaat
    31081 tcgtcaccgc cgaagcggta aattttacct ttatctaccg ggatctgtga tttaaggatt
    31141 ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt
    31201 ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt tttccggcag cgcttttaaa
    31261 tcttcgtcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta
    31321 tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg
    31381 cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg
    31441 cgtaccagat agaggcaact ggtacccgat atcactgcca ccaatgcggc aaagaaaata
    31501 atcaatgtac agacccgcaa ataaacatct tttttatacg caaatacgta caatccggtc
    31561 ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcataa
    31621 tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg
    31681 tccatcgcca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga
    31741 ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata
    31801 aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt
    31861 ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg
    31921 tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt
    31981 tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca
    32041 atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc
    32101 tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga
    32161 ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt ttactaattt
    32221 tccactttta tcccaggcgg agaatggcat gggtttctgg tacagatacc cctgagcctg
    32281 aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat
    32341 caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt
    32401 accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata
    32461 aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg
    32521 tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc
    32581 agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa
    32641 acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa
    32701 atcatccttc agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat
    32761 aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag
    32821 cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat
    32881 ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat
    32941 tgcatggcga atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc
    33001 atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag
    33061 tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg
    33121 gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg
    33181 ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac
    33241 gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt
    33301 attgcgttca tcaacaccaa acaccgcttc cacggcttcc acggcttcca gaccacacag
    33361 tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg ccatattttt
    33421 aataaaaccg ctttcctgat cgtaagagat cagtgagagc aatttatttg accgttcaat
    33481 ttcggcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt tcgcatcgcc
    33541 ttcgcgaatt ttcagagcct cgatctgatc cgttgagtcg ggcaaaaacg ccagcgtatt
    33601 aaccagctcg gtcatttcag aaaattgccg acggaacaca cgagtggtca tgaaatatac
    33661 caccagcaac acgataataa tgaacgcaaa cggtagataa aaaagtttat ctgatgcgtt
    33721 caataaatta ccgccaggat aataaaaata gatttcgaac aatgcattat ttggcactgc
    33781 tgatgtcttt gcaatatact ccgtatcgac agaatcaata atattataat taccttcgct
    33841 attataaata atcagttttt cacgagcgag ttgattcttg gcaatgatct catgaccttg
    33901 tttcaaaaat agaacttcat cattagcgct gatttgatca agatactcgt tcagataccg
    33961 ccagtttaaa tcgacatgaa ttttatctac cgctaacaaa tcgtgatctt tgccgttgat
    34021 acgtagcgta aaagagatac attttttacc cggacgatca aagcaattgt atgccggatt
    34081 ccagctgttg ttagagtcat ctgaagccac cgaccacggt cgtttcgatg ggttaaaatc
    34141 ggcaaaaata acccgccctg actcgtcgac aagcggtcca tttacttgtt cctgatgata
    34201 aacaacgatt ttatcattcg ccctccgtga aaagagataa tatttattat catcaagtac
    34261 cagtcccacc gagtctaaaa atggcatcat ttccattttc agaatcacgg cgcgttgaat
    34321 ctcctctgta ttttttaata aatacagtga gtcctttaat gtcagggc
//
LOCUS       DAZUFB010000045        33384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZUFB010000045 DAZUFB010000000
VERSION     DAZUFB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.045"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            102..686
                     /gene="yraH"
                     /locus_tag="ACHY3M_002415"
     CDS             102..686
                     /gene="yraH"
                     /locus_tag="ACHY3M_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317320.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            766..1461
                     /gene="yraI"
                     /locus_tag="ACHY3M_002416"
     CDS             766..1461
                     /gene="yraI"
                     /locus_tag="ACHY3M_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4317321.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1491..4007
                     /gene="yraJ"
                     /locus_tag="ACHY3M_002417"
     CDS             1491..4007
                     /gene="yraJ"
                     /locus_tag="ACHY3M_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4317322.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4018..5109
                     /gene="yraK"
                     /locus_tag="ACHY3M_002418"
     CDS             4018..5109
                     /gene="yraK"
                     /locus_tag="ACHY3M_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317323.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTNRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5152..6012)
                     /gene="rsmI"
                     /locus_tag="ACHY3M_002419"
     CDS             complement(5152..6012)
                     /gene="rsmI"
                     /locus_tag="ACHY3M_002419"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4317324.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6076..8112
                     /gene="lpoA"
                     /locus_tag="ACHY3M_002420"
     CDS             6076..8112
                     /gene="lpoA"
                     /locus_tag="ACHY3M_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFS4317325.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8070..8465
                     /gene="yraN"
                     /locus_tag="ACHY3M_002421"
     CDS             8070..8465
                     /gene="yraN"
                     /locus_tag="ACHY3M_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFS4317326.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8485..9075
                     /gene="diaA"
                     /locus_tag="ACHY3M_002422"
     CDS             8485..9075
                     /gene="diaA"
                     /locus_tag="ACHY3M_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFS4317327.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9085..9660
                     /gene="dolP"
                     /locus_tag="ACHY3M_002423"
     CDS             9085..9660
                     /gene="dolP"
                     /locus_tag="ACHY3M_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS4317328.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9774..10814)
                     /gene="yraQ"
                     /locus_tag="ACHY3M_002424"
     CDS             complement(9774..10814)
                     /gene="yraQ"
                     /locus_tag="ACHY3M_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4317329.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10887..11522)
                     /gene="yraR"
                     /locus_tag="ACHY3M_002425"
     CDS             complement(10887..11522)
                     /gene="yraR"
                     /locus_tag="ACHY3M_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS4317330.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11650..12168
                     /gene="yhbO"
                     /locus_tag="ACHY3M_002426"
     CDS             11650..12168
                     /gene="yhbO"
                     /locus_tag="ACHY3M_002426"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFS4317331.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12148..12591)
                     /gene="yhbP"
                     /locus_tag="ACHY3M_002427"
     CDS             complement(12148..12591)
                     /gene="yhbP"
                     /locus_tag="ACHY3M_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFS4317332.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12642..12944
                     /gene="yhbQ"
                     /locus_tag="ACHY3M_002428"
     CDS             12642..12944
                     /gene="yhbQ"
                     /locus_tag="ACHY3M_002428"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFS4317333.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12931..13434)
                     /gene="yhbS"
                     /locus_tag="ACHY3M_002429"
     CDS             complement(12931..13434)
                     /gene="yhbS"
                     /locus_tag="ACHY3M_002429"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4317334.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13428..13952)
                     /gene="ubiT"
                     /locus_tag="ACHY3M_002430"
     CDS             complement(13428..13952)
                     /gene="ubiT"
                     /locus_tag="ACHY3M_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFS4317335.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14161..15156
                     /gene="ubiU"
                     /locus_tag="ACHY3M_002431"
     CDS             14161..15156
                     /gene="ubiU"
                     /locus_tag="ACHY3M_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4317336.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15165..16043
                     /gene="ubiV"
                     /locus_tag="ACHY3M_002432"
     CDS             15165..16043
                     /gene="ubiV"
                     /locus_tag="ACHY3M_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4317337.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16249..17256
                     /gene="yhbW"
                     /locus_tag="ACHY3M_002433"
     CDS             16249..17256
                     /gene="yhbW"
                     /locus_tag="ACHY3M_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFS4317338.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17374..18618)
                     /gene="mtr"
                     /locus_tag="ACHY3M_002434"
     CDS             complement(17374..18618)
                     /gene="mtr"
                     /locus_tag="ACHY3M_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFS4317339.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18772..20661)
                     /gene="deaD"
                     /locus_tag="ACHY3M_002435"
     CDS             complement(18772..20661)
                     /gene="deaD"
                     /locus_tag="ACHY3M_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFS4317340.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLNLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20654..20734)
                     /gene="yrbN"
                     /locus_tag="ACHY3M_002436"
     CDS             complement(20654..20734)
                     /gene="yrbN"
                     /locus_tag="ACHY3M_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFS4317341.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20841..21725)
                     /gene="nlpI"
                     /locus_tag="ACHY3M_002437"
     CDS             complement(20841..21725)
                     /gene="nlpI"
                     /locus_tag="ACHY3M_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFS4317342.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21834..23969)
                     /gene="pnp"
                     /locus_tag="ACHY3M_002438"
     CDS             complement(21834..23969)
                     /gene="pnp"
                     /locus_tag="ACHY3M_002438"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4317343.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24216..24485)
                     /gene="rpsO"
                     /locus_tag="ACHY3M_002439"
     CDS             complement(24216..24485)
                     /gene="rpsO"
                     /locus_tag="ACHY3M_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4317344.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24634..25578)
                     /gene="truB"
                     /locus_tag="ACHY3M_002440"
     CDS             complement(24634..25578)
                     /gene="truB"
                     /locus_tag="ACHY3M_002440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4317345.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25578..25979)
                     /gene="rbfA"
                     /locus_tag="ACHY3M_002441"
     CDS             complement(25578..25979)
                     /gene="rbfA"
                     /locus_tag="ACHY3M_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4317346.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26143..28815)
                     /gene="infB"
                     /locus_tag="ACHY3M_002442"
     CDS             complement(26143..28815)
                     /gene="infB"
                     /locus_tag="ACHY3M_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4317347.1"
                     /translation="MTDVTIKTLAAERQTSVEGLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28840..30327)
                     /gene="nusA"
                     /locus_tag="ACHY3M_002443"
     CDS             complement(28840..30327)
                     /gene="nusA"
                     /locus_tag="ACHY3M_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4317348.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30324..30809)
                     /gene="rimP"
                     /locus_tag="ACHY3M_002444"
     CDS             complement(30324..30809)
                     /gene="rimP"
                     /locus_tag="ACHY3M_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4317349.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVFPTFNSLDEVKSP
                     R"
     gene            complement(31016..31092)
                     /locus_tag="ACHY3M_002445"
     tRNA            complement(31016..31092)
                     /locus_tag="ACHY3M_002445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31056..31058),aa:Met,seq:cat)
     gene            31440..32783
                     /gene="argG"
                     /locus_tag="ACHY3M_002446"
     CDS             31440..32783
                     /gene="argG"
                     /locus_tag="ACHY3M_002446"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFS4317350.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32791..>33384)
                     /locus_tag="ACHY3M_002447"
     CDS             complement(32791..>33384)
                     /locus_tag="ACHY3M_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4317351.1"
                     /translation="PHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDD
                     QDACYDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQ
                     AYHVPMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHY
                     KGDSRVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         8166 a         8999 c         8286 g         7933 t
ORIGIN      
        1 aatgttcaat gaaatatttc tacctgtata atctttaaag atgttgaaca tatattcaca
       61 ttaaatatga ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa
      121 cagctattgt gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa
      181 tagtttttga tggcgagatt ttaaaatccg cttgtgaaat caatgactct gataagaaaa
      241 ttgaagttgc tcttggtcac tataatgccg aacagtttcg ttctgttggc gatcgtagcc
      301 caaaaattcc attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata
      361 acggcaacgt tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggcaccgtcc
      421 ctaacttccc taatctggca aaagtgggct cttttgctgg cacagcggca acaggtgtgg
      481 gtatccgtat tgacgatgcg gaaagcggaa accttatgcc actgaatgct atggggaatg
      541 ataacacggt ttatcagatc ccagcggact ccaatggtat tgtcaatgtt gacctcatcg
      601 cttactacgt atcaaccgtt gaagcatcag aaatcacccc aggggaagca gacgctgtcg
      661 ttaacgtaac gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg
      721 cccttttcct tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca
      781 ttcgcggtga taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt
      841 atcgttttac agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct
      901 gtcgcaaaca aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat
      961 ggtacaagcc gtgccccatt tattataacc ccaccgctat ttcgtcttga agccggcgat
     1021 gactcatcac tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg
     1081 ttctacatta atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac
     1141 gagttgacgc tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag
     1201 ggacgggtaa acgatgcgtg gacatcactg gaatttaaac gtagtgacca ttcactcaat
     1261 atatataacc caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat
     1321 ctcacaagca aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca
     1381 tctggcggaa agaacgtgaa atgggctgca atcaatgatt atggcggcag ttccgggaca
     1441 gaaactcgtc cactgcaata acaaatataa aaaacacagg tcatcaggga atgccacaac
     1501 gacaccacca gggacataaa cgcacaccga aacagttggc gctcatcatc aaacgctgtt
     1561 tgccgatggt gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt
     1621 atttcgaccc cattatgctg gaaactacaa aaagtggtat gcaaacaact gatctgtcac
     1681 gtttttcaaa aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata
     1741 aaaagaaggt ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc
     1801 cacagtttac ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc
     1861 tggctgaaaa agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag
     1921 ctgctgaatt tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt
     1981 accgtgatgc aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt
     2041 ttaccaacta ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac
     2101 gacagtacct gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt
     2161 ctacgtggac acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac
     2221 gtgatatcaa ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta
     2281 ttttttccag ctacaccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa
     2341 acagccagcg cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga
     2401 ctatcaggca aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa
     2461 ttaacgatct ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg
     2521 acggtacgca acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg
     2581 ggcatctaaa atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca
     2641 aggaacccga atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt
     2701 atggcggcct gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac
     2761 ttggcgcact gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc
     2821 ggcactcttt tcatggctat caatggcgta cgcagtacat caaagatatc ccggaaacca
     2881 acaccaatat cgctgttagc tactatcgct ataccaacga tggctatttt agttttgatg
     2941 aagccaatac ccgtaattgg gactataaca gtcgccaaaa aagtgaaatt caattcaaca
     3001 tcagccagac aatatttgat ggggtaagtc tgtatgcctc cggttcgcag caagactatt
     3061 ggggcaataa cgataaaaac aggaatatct cagttggggt ttccggtcag caatggggaa
     3121 ttggttacag cctgaattat caatacagtc gctacacgga tcaaaataat gaccgcgcac
     3181 tctctttgaa tctcagtatt ccgttagaac gctggttacc gcgtagccgg gtttcctatc
     3241 agatgaccag ccagaaagat cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc
     3301 tggatgatgg tcgcctgagc tatagcctgg aacaaagtct ggatgacgat aacaaccata
     3361 acagtagcct gaacgccagt taccgttcac cttatggcac cttcagtgcc ggatacagct
     3421 acggtaatga cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc
     3481 atggcgtgac gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaatgggg
     3541 cttctggcgt gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag
     3601 tggttcctta tctcacgact taccaggaaa accgtctctc ggtagatact acgcagctgc
     3661 ccgataacgt cgatcttgag caaacaacac agtttgtggt gcccaacaga ggtgcaatgg
     3721 tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca
     3781 acggtaaacc gttgcccttt ggcgctcttg ccagcaacga tgagacgggg caacaaagta
     3841 tcgtcgatga gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga
     3901 ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt
     3961 cagaaccaac aacctctgta ttacaaggca cggcgcagtg ccattaagga taaaaaaatg
     4021 aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc
     4081 tcaggagggt gtggagctga cagcactagc ggtgcgacaa attacagcag tgtggttgat
     4141 gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc tgccacgcta
     4201 agtagcacta actggcaata cgcctgtacc tgctctgcgg gtaaggcagt taaacttgtc
     4261 tatatggtca gccctgtact taccaccact ggacatcaag caggatatta caaactcaat
     4321 gatagtctgg atattaaaac cacattacag gcgaatgaca ttccaggact cgtgactgac
     4381 cagaccgttt ctgttaacac ccgattcaca cagataaaaa gcaacaccgt atattctgct
     4441 gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt
     4501 gcaaatacca cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg
     4561 aaaacggata ttgccgtcat taaaggcgca tgggtcgatg gaatgggaag cccgtctaca
     4621 ggtgacttcc atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg
     4681 tgcaaaatta atcagggcga tgttattaag gttaattttg gattcatcaa tggtcagaag
     4741 tttaccaacc gcaatgccat gccagacggt tttactccag tagactttga tatcacttat
     4801 gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact
     4861 ggggtagtag accagtacaa cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat
     4921 gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt
     4981 atccttcccg ttgatccttc cgggcatggc accatcaaca tgcgcgcctg gccagttaat
     5041 ctggtcggtg gtgagctgga aacaggaaaa tttcagggca ccgccaccat taccgtcatc
     5101 gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg cttcggcact cttacccctg
     5161 ctgctccagc gcatacttat acagcgcatt tttcttcacg ccgtgaattt ctgccgccag
     5221 cgccgccgct tttttcaacg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc
     5281 ggcgggtaag tcttcttcct gtgctttatg accttcgata atcagcacca tttcgccttt
     5341 gcgacggttt tcatcttctt ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat
     5401 ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac
     5461 cgcaacgata tcttccaggc tatctaacag acggtgggtg gattcataaa aaatcagcgt
     5521 gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa
     5581 aaagccttcg taacagaaac ggtcagaggg taaacccgcg gcgcttaacg cagtgatagc
     5641 agcacacggc ccgggtagcg gcaccacgca aatccccgct tcacggcagg tacgcaccag
     5701 atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttctg
     5761 cccttcttgc agcttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag
     5821 cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc
     5881 ctcggcggca atcagatcaa cggcctgtaa tacctctaac gcacgctggg tgatatccgc
     5941 cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg
     6001 gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaat
     6061 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     6121 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     6181 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     6241 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     6301 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     6361 tctcagcgtc gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt
     6421 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     6481 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     6541 cgcgcgttaa ttgctcagga accgctgctc ggcgcgaaag aaaaacagca gaatattgat
     6601 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     6661 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     6721 aacgatcccg acatgatgaa agccgggatc gccgactggc aaaaacgtta tccgaacaat
     6781 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     6841 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     6901 attcagcaag gctttgaagc ggcgaaaaat atcggcactc agccagtggc ggctcaggta
     6961 gctgccgcac ctgccgcaga cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt
     7021 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg aacagcctgc ggcacagcca
     7081 gcgcctgtaa gcaccccggc agcaaccacc acagcggtaa gcgcgccagc gaatccatcc
     7141 gcagaactga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     7201 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     7261 cttaagagca acaccccgct gaacgtgctg gcactgaacc agccggagaa tatcgaaaac
     7321 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     7381 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc
     7441 gatcgcgtag ccaatgcgtt tgcgcaggag tggcagaaac tgggcggcgg caccgttctg
     7501 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc
     7561 gctttaacgg gtaccccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     7621 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     7681 gatgcggtgt acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     7741 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gctcccgcag tgcgcaaggg
     7801 accgctggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     7861 ctggcaggcg gtaatctacc gttaatgcag caggcactca gcgcggtgaa taacgattat
     7921 tcactggctc gcatgtatgc gatgggcgtc gatgcctggt cgctggcaaa tcatttctca
     7981 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagcct gacggcaaac
     8041 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     8101 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agtacaagcg cgtcgctggc
     8161 tggaaggcaa aggactgcga tttgtcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     8221 atctgataat gcgtgaaggc cggaccaccg tttttgtcga ggtacgctat cgccgctctg
     8281 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     8341 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     8401 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     8461 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     8521 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     8581 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     8641 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     8701 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     8761 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     8821 ttgttagcca tttccacccg tggcaacagc cgcgatattg taaaagcggt agaagctgcc
     8881 gttacgcgtg atatgactat tgtggcattg accggctatg acggcggcga acttgcaggt
     8941 ttgttagggc cacaagatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     9001 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     9061 caccaggatg tttaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     9121 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     9181 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     9241 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaagcccg tattaatgtc
     9301 acggcgtatc agggcaaagt gctgctggtt gggcagtcac caaacgctga actttcggct
     9361 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     9421 cagggccagc cgattggtct gggcgaagcg tctaacgata cgtggatcac caccaaagtg
     9481 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     9541 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     9601 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     9661 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     9721 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     9781 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     9841 aaggctttcg ctgggaacgc tttgcgcagc ataatcagtg acggcaaact caccgccggg
     9901 agcgtcatca acaatgccag cgccggagca gttcccatac ctgccagcat catcgtttgt
     9961 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
    10021 atcacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga
    10081 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc
    10141 catagcgccc tgccccagcg gctaaaaaaa ccgccctgtg cttccggtat gtcaatttcg
    10201 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
    10261 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
    10321 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
    10381 ccgcccatcg acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
    10441 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
    10501 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
    10561 acccccagca ctgccgcttt ccataccgcg aggaaataga tcatcgcgta atccaacgcc
    10621 gcctgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
    10681 gcagcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
    10741 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
    10801 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
    10861 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
    10921 aaaatcgtca caccttcatg cccgggccgc atcgcctctg ccagcatgac ccgcgccacg
    10981 tctctcgcat caatggattt ccagttaccg ggtaataaac ggaacagcgg cgcaaaaagc
    11041 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
    11101 aatttcggcc agttctgggc gattaatgct tcttccatct cccctttgac gcggttatag
    11161 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
    11221 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
    11281 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga
    11341 tcggtgacct gcgccagcgc gtcagtcagt tgcggatcat ggggattaaa caccccgggc
    11401 atatcgccca acggacgtcg cgtcggcgcg gctatggcgt taactttcgg ttcgttaatc
    11461 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
    11521 atcttcgctc ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccaggcttaa
    11581 tagaccatga ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc
    11641 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
    11701 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa
    11761 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
    11821 atcgatgaag tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg
    11881 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
    11941 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
    12001 gggcgcaaac tgaccgcggt taaaccgatc attattgatg ttaaaaacgc gggcgcggaa
    12061 ttttacgatc aggaagtcgt ggtcgataaa gatcagctgg ttaccagccg gacaccggac
    12121 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagc cgcgtagcca
    12181 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
    12241 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
    12301 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacgccgcg
    12361 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
    12421 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
    12481 atcaaacaga taaaaagcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
    12541 gacaacatgt tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct
    12601 cttttatact gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt
    12661 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
    12721 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
    12781 cgctggcgtt ttctgcgcca gtgggcgatc gttcgctggc gttacgggcg gaatatcgcg
    12841 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
    12901 agctgttaag cagtctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
    12961 aaccagacca gttacgccat tcaacgcgtc atccgctaaa cgatgtacct ggaaggcgct
    13021 ttcagtgcct ggccagcggc aacgcaggtc gtgatgtgcc gccagttcaa agccaaaacg
    13081 gctgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
    13141 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
    13201 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
    13261 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
    13321 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
    13381 tagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
    13441 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
    13501 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
    13561 aggtttttca catacagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
    13621 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
    13681 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
    13741 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
    13801 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
    13861 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
    13921 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
    13981 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
    14041 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
    14101 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
    14161 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
    14221 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
    14281 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
    14341 cttcacatcg cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
    14401 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgcta
    14461 gagtacgccg ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg
    14521 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgctcg cgtggtgctg
    14581 ccgcgcgtgt tgtcgattca tcaggtgaaa caactggcac gggtcacacc tgtaccactg
    14641 gaagtctttg ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg
    14701 tatctgacgg gtgagtcgcc caacaccgta ggcgcgtgtt ctccggcccg tttcgtgcgc
    14761 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
    14821 caggacggcg aaaacgcagg ttatccgacg ctatgtaaag ggcgttatct ggtggacggc
    14881 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    14941 ttaatggcgg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    15001 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    15061 aacttcatac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcag
    15121 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    15181 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caggccgcca
    15241 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    15301 aagttggcga ctggctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    15361 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    15421 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    15481 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    15541 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    15601 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    15661 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    15721 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    15781 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    15841 acgtttacaa cctcggtaac gagctggcat ccatgcaggg gctggttgat gtggtccgcc
    15901 tgtcaccgca gggtactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    15961 gcgctgcgcc actaccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    16021 ccgggctgga gctgcaagcc taagtaaata tgctcgtcat acttcaagtt gcatgtgctg
    16081 catctgcatt cgttcacccc agtcacttac ttatgtaagc tcctggggat tcactctctt
    16141 gtcgccttcc tgcaactcga attatttaga gcacagctca ctttgttaac aactttaact
    16201 actctttaat gcagtattaa agattaatcg gtaacaaagt gagctgttat gactgataaa
    16261 accattgcgt tttcgctact cgatctggcc cccattcccg aaggttcttc agcgcgagaa
    16321 gcattctccc actctctcga tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac
    16381 tggctggcag aacaccacaa tatgactggc attgccagtg ctgccacgtc ggtattgatt
    16441 ggctatctgg cggcgaatac caccacgctg catctggggt ctggcggcgt gatgttgcct
    16501 aaccactcac cgttggtcat tgccgaacag ttcggcacgc tcaatacact ctatccgggg
    16561 cgaatcgatt tggggctggg tcgtgcgcca ggtagtgacc aacggacaat gatggcgctg
    16621 cgtcgccata tgagcggcga tattgataat ttcccccgcg atgtcgcgga gctggtggac
    16681 tggtttgacg cccgcgatcc caatccgcat gtgcgcccgg taccaggcta tggcgagaaa
    16741 atccccgtgt ggttgttagg ctccagcctt tacagcgcgc aactggcggc gcagcttggt
    16801 ctgccgttcg cgtttgcctc acacttcgcg ccggatatgt tgttccaggc gctgcatctt
    16861 tatcgcagca acttcaaacc gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc
    16921 aatattatcg ccgccgatag caaccgcgac gctgagtttc tgtttacctc aatgcagcaa
    16981 gcctttgtga agttgcgccg tggcgaaacc gggcaactgc cgccaccgat tcaaaatatg
    17041 gatcagttct ggtcgccgtc cgaacagtat ggtgtgcagc aggcgctgag tatgtcgttg
    17101 gtaggtgata aagcgaaagt gcgtcatggc ttgcagtcga tcctgcgcga aaccgacgcc
    17161 gatgagatta tggtcaacgg gcagattttc gaccaccagg cgcggctgca ttcgtttgag
    17221 ctggcgatgg atgttaagga agagttgttg ggatagtgtg tcttaacgcg ggaagcctta
    17281 tccgagctgg caacgctgtc ctacgtagac ctgataagcg aagcgcatca ggcattgtgt
    17341 aggcagcaga aatgtcggat aaggcaccgc tgattactga tacaccggca gtaaattaaa
    17401 gctcgataaa atatggacca gtgcgttgcc aacgccaaac accagaatca gcgtaatcat
    17461 cggcttgccg ccccagacgc ggaatttcgg gctgccaaag cgtttacgcg atgcgcgggc
    17521 taacagcgcc ggaacaattg ccgcccagat ggtagctgct aaaccagcat aaccaatggc
    17581 gtacaggaat ccgttcggga acagtagacc acccacaaca ggcggggcaa aggtcagcaa
    17641 tgccgttttc aagcgcccca gggctgagtc atcgaaacca aacagatctg ccagatagtc
    17701 aaacaaaccc agcgttacgc caaggaacga actcgctacc gcaaagtttg agaacacgac
    17761 cagcagaagg tccaggctac ggctgttcag cacgccgctt aatgcctgta ccagcacatc
    17821 aatattaccg cccttctctg caataccgat aaactccgga cgcgggatgt tacccatcgt
    17881 cgccagcaac cagatggtat acagcgccag tgccatcagc gtaccgtaca ccaggcattt
    17941 cacgatggtt ttcggatctt tgccgtaata cttcatcagg cttggcacgt taccgtgata
    18001 accaaacgac gccaggcaga acggcaatgt catcagcaga tacggcgcat aagatgcatt
    18061 gctttctgca acgttgaaca atgtcgcagg ctgcacatgc cccaacaggc tgccaaaggt
    18121 gaggaagaag gtaatgactt tcgcccccag cacaatcgcc gtcatgcggc tgacggcctt
    18181 agtactcaac cacaccacaa acgctaccag cagtgcaaaa ccaaaacccg ccgcccgtgc
    18241 cgggacgttt agcgacatct ctgcgaaggt gtgatgcaga attgaaccac tggcagaaat
    18301 ataggcatag gtcaggatat agagcacaaa ggcaatggaa ataccgttga ccacgttcca
    18361 gcctttgccc agcaaatctt tggtgatggt gtcaaaactc gaaccgattc ggtaattcag
    18421 gttagcttcc agaatcatca agccggaatg cagcatacag aaccaggtaa agatcagcgc
    18481 cgccattgac cagaaaaacc acgccccgga catgaccact ggcagagaaa acatccctgc
    18541 gccaataatg gtgccgccga taatcaccac gccgccaagc agcgacggtg acgtttgggt
    18601 ggtggttagt gttgccatga gggcttctct ccagtgaaaa atagtgcgac tgcgttgtta
    18661 tgcattgcac tgtaccagta cacgagtaca aaagacagaa aaaaagcccc gatggtaaaa
    18721 atcggggctg tatatattat tttacagatt gtgttcgctg ttcagcgatg attacgcatc
    18781 accaccgaaa cgacgacgac cggtagagtc atcacgacgc ggagcgcggc cttcacgacg
    18841 ttcgccgcta aaacgacgac catcaccacg gccaccttca cggcgttcac cgctgaagtt
    18901 acgaccgcct tcacgacgtt cgccaccgaa accacgacca ccgccacgac gctcaccgcc
    18961 agtatgcggc tgcgcatcgc ccagcaactg catattcatc ggcttgttca gaatgcgagt
    19021 acgcgtaaag tgttgcagta cttcacccgg catacctttc ggcagttcga tagtggagtg
    19081 agaagcaaac agcttaatgt taccgatgta acggctgctg atgtcgcctt cgttagcaat
    19141 cgcgccaacg atatgacgaa cttcaacacc atcatcgcgg cccacttcaa tgcggtaaag
    19201 ctgcatgtca ccaacatcac gacgttcacg acgcggacga tcttcacggt cgccacgtgg
    19261 gccacggtcg ttacgatcgc gcggaccacg gtcatcacgg tcacggaatt cacgtttcgg
    19321 acgcatcggc gcatctggcg gaacaatcag agtacgttca ccctgtgcca ttttcagcag
    19381 tgccgcagcc agagtttcga gatcctgctc ttcaccttct gcagtcggct gaattttgct
    19441 cagcagtgcg cggtattgat ccagatcgct gctttccagc tgctgctgta ctttagcggc
    19501 gaatttttcc agacggcgtt tgcccagcag ttctgcgttc ggcagttcta cttccggaat
    19561 agtcagcttc atagtacgtt caatgttgcg cagcagacga cgctcgcggt tctcaacgaa
    19621 cagcagcgcg cggccagcac gacccgcacg accggtacga ccgatacggt gaacgtaaga
    19681 ctcagaatcc atcgggatat cgtagttaac taccaggctg atacgctcaa cgtccaggcc
    19741 acgggctgca acgtcggtcg caatcaggat gtccagacga ccatctttca ggcgttccag
    19801 tgtctgttca cgcagcgcct ggttcatgtc accgttcagc gcggcgctgt tgtagccgtt
    19861 acgctcaaga gcttcagcca cttccagagt cgcgtttttg gtacgaacga agataatcgc
    19921 cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct tcgtttttgc gcatacccca
    19981 gacagtccag tagctctggc tgatgtcagg acgggtagtc acgctggact gaatgcgcac
    20041 ttcctgcggc tctttcataa agcggcgggt aatgcgacga atcgcttccg gcatggttgc
    20101 agagaacaga gcggtctgat gaccttccgg gatctgcgcc ataatggttt caacgtcttc
    20161 gatgaagccc atgcgcagca tttcgtcagc ttcatccaga accagaccgc tcagtttaga
    20221 gaggttcaga gtgccacgtt tcaggtggtc cagcagacgg cccggagtac cgacaacgat
    20281 ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa cgctggccgc cgtatagagc
    20341 aaccacattt acgccgcgca tgtgtttaga gaaatccgtc attgcttcag caacctgtac
    20401 cgccagttcg cgggtcggtg ccagcaccag aatctgtggt gctttcagct caggatcaag
    20461 attctgcaac agaggtaaag agaatgctgc agtttttccg ctccccgtct gggccatacc
    20521 cagaacgtcg cggccattca gcagatgtgg aatacactct gcctgaattg gagatggttt
    20581 ttcgtaaccc agatcgttaa gggcttcaag gataggagcc ttcaggccca gatctgcaaa
    20641 agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa attaatggcg gccagtctac
    20701 ataactcatc atgaaattga tcagcaattt tcattgaaaa gtgtgaaccg gctcaaagta
    20761 ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg atggcaatca aaaaagatta
    20821 cgggctgatg tgtacgtcag ctattgctgg tccgattctg ccaggtcatc ttggtcctgg
    20881 cccaggagcg ataattccaa caatgcgtat cggtgctcaa caaagttatg aacgttgttg
    20941 gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat cccccagact taggtagtac
    21001 ttacctaaat agaagttggt ttcactgaga tgctcagcga gcgaggtgtt atccgttgcg
    21061 tccgccttga gcctttccat taacgtttgt tcgctaatgt tgcccaggta gaactcgaca
    21121 atgttccatc cccactgttc cttatccgat ttttcgaagt gctgtttcaa cacttcttta
    21181 gcctgcttct catcgagctt ctgctcggcg agataaagcc acagactacg gaaaggatca
    21241 ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg ctaacttgtc acgaccgccg
    21301 taatataatg cgatcccgcg attcaagtgc gcgtagttgt aagttggatc aagctcaagt
    21361 acagaatcaa acgcttcata ggcagcatca aaattgcctg cctgcgttaa atatatgcct
    21421 aagtaattga atacttcagg catatccggt cggattgcca gcgcttgcga aaaatcgtta
    21481 cgcgctaatg ccctcagacc gagactatca tacaacactc cgcgctcata taaaagctgt
    21541 gcgcgttcgt catcggttaa agcccgactg gcaaggattt gttccatacg tgccagaatc
    21601 acttcctgct gtaaagtcgg ttgcaatggt accgcgagga cttcactttt acgccaggaa
    21661 gtattactgc atcctgcaag cgtaagtgct gtcgcaacga aacaccagcg caaaaaaggc
    21721 ttcatttccc actcccgaag accacggttg aatgaacgtc ctgttcccgg ttgctaacaa
    21781 ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg gcaaatggca accttactcg
    21841 ccctgttcag cagccggagc ttccggtgct gcagcaggtt gagactgctc agtcgcttct
    21901 ttaatgctca gacggatacg gccctggcga tcaacttcca gaactttcac cggtacttcc
    21961 tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt tgtcagcgat ttgagagatg
    22021 tggaccagac cttctttacc gccgccgatg gcaacaaatg cgccaaagtc aacgatacgg
    22081 gtcactttac cagtgtagac gcggcccact tcgatttctg cagtgatctc ttcgatacga
    22141 cgaatagcat gtttcgcttt ctcgccgtcg gtcgctgcga tcttcacagt accgtcatct
    22201 tcgatttcga tggtagtgcc agtttcttcg gtcagggcac ggataacaga gccgccttta
    22261 ccgataacgt ctttgatctt gtccgggttg atcttgatgg tatggatacg cggtgcgaac
    22321 tcagagatat cgccacgcgg cgcgttgatc gcctgttcca ttacgcccag gatatgcaga
    22381 cgcgcacctt tagcctggtt cagcgcaacc tgcatgatct ctttggtgat accttcaatt
    22441 ttgatatcca tctgcaatgc agagataccc tcgcgggaac ccgcaacttt gaagtccata
    22501 tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta caacgtagtt gtcgccttct
    22561 ttcaccagac ccattgcgat acccgcaacg gcagctttga tcggcacacc tgcgtccatc
    22621 agcgccagag acgcgccgca cacggaagcc atagaagagg aaccgttgga ttcagtgatt
    22681 tcagacacaa cacgtacggt gtacgggaat ttgtccatat ccggcatgac tgccagcacg
    22741 ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct tcggagaacc gaccatgccg
    22801 gtttcgccta cggagtacgg agggaagttg tagtggaaca ggaaggtatc ggtacgttcg
    22861 cccatcagtt catcaagaac ctgcgcgtca cgagcagtac ccagcgttgc ggtaaccagc
    22921 gcctgcgttt caccacgggt gaacagcgca gaaccgtgag tacgcggcag cacgccagta
    22981 cgcacatcca gaccacggat catatctttt tcacgaccgt cgatacgcgg ttcgcctgcc
    23041 agtacgcggc tacgaacaac gtttttctcg atagcgtgca gaatttcacc cagttcgttt
    23101 tcgtccaggg tttcgtcttc agcaagcagc gtcgcgatgg tttcagattt gatgacatca
    23161 acctgcgcat aacgctcttg tttgtcggtg atgcggtaag catcgctcag gcgagcttca
    23221 gccagtgcag caacgcgcgc gttcagcgct tcgtttaccg gctccggctg ccagtcccaa
    23281 cgtggtttac cggcttcttt caccagttca ttgatgttct gaataacaac ctgctgttgt
    23341 tcatgaccga acactactgc gcccagcatc tggtcttcgc tcagcagttc agcttcagat
    23401 tcaaccatca gaacagcggc ttcagtaccg gcaacaacca gatccagttt gctctctttc
    23461 agctcgtcct gagtcgggtt cagtacgtac tggtcattga tgtaacctac gcgagcagca
    23521 ccaatcggac cattgaacgg aataccagac agagacagtg ctgcggaagc accaatcatc
    23581 gcgacgatat ccgggttaac ttgcgggtta acagaaacca cggtggcgat aacctgaact
    23641 tcgttgacga agccttccgg gaacagcggg cgaatcgggc ggtcaatcag acgcgcgatc
    23701 agggtttcgc cttcgcttgg gcggccttca cgacggaaga agctacccgg gatacgacca
    23761 gcagcgtagg tacgctcctg atagttaacg gtcagtggga agaagtcctg acctggtttg
    23821 gcttttttct ggccaacaac ggtaacaaat accgcggtgt catccatgct aaccataaca
    23881 gcggcagtag cctgacgagc catcatgccg gtttccagag tcacggtgtg ttggccgtac
    23941 tggaatttac gaacgatcgg attaagcaat gtaatatcct ttctctttct tagacagtac
    24001 cttacggcac tggtgttaat acccgatctt ctgcgcatcc tcgcgactaa tgacaaccct
    24061 aacccagctc tatgtgggta aagcctctca ttagccgcgc gaacctctgc aacggaagat
    24121 cattcatagc aacaatacat tagtttccag tgaattgctg ccgtcagctt gaaaaaaggg
    24181 gccactcagg cccccttttc tgaaactcgc aagaattagc gacgcagacc caggcgctcg
    24241 atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt agtcgagcag tttacgacgc
    24301 tgagaaacca tgcgcagcag accacgacgg ctgtggtgat cttttttgtg ctctgcaaag
    24361 tggccctgca ggtggttgat ctgtgcagtc agcagtgcta cctgaacttc ggtagaaccg
    24421 gtgtcgtttg cgtcacgacc aaactcagaa acgattttag ctgttgcttc agtacttaga
    24481 gacattttaa aactccaaag tatatagaat gaaaggacgc cgatctctaa ttcagcgatc
    24541 ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac gttaagcggc agtattctac
    24601 tcgtagcgac ctgttatcgc aagacggtta acattacgcc gggtattcaa ccaccaggcg
    24661 acgaggcgca acgcggcctt catcgtcaat ttcgcccata ccgataaatt tgccgttctc
    24721 accttctgta acgcgaacca gtccttccag cggcgcaccg gatgtacgaa ctgggttacc
    24781 atttttgaag taaacagaag acgttaacgg aagattcacc accgggtagt ccgaagctgg
    24841 actgtccatt ggcatcagta atggatcaag taactccgca gccggaatat cctgctgttc
    24901 ggcttgttcg accagttcac gcagatgttc cagggtcacc atccgttcaa ccggatattt
    24961 acttaccgcc agacgacgca ggtaaataac atgcgcgcca caaccgagtt tttcacccag
    25021 gtcatcaatg atggtgcgga tataagtgcc ttttgagcag tgaatttcca gctccagctc
    25081 attgccttcg tggcgaataa acagcaattc ataaacggta atcgggcgcg cttcacgcgg
    25141 tacttcaatg ccctgacgcg catattcgta cagtttttta ccctgatatt tcagtgctga
    25201 atacatcgaa gggatctgtt cgatatcgcc acggaaagta tccagcgccg ccgccagttg
    25261 ctctgcgcta aaggttaccg gacgttcttc aacgatttgt ccgtcggcat cagaagtatc
    25321 ggtacgctgt ccaagacgcg caatgacccg atagcgtttg tcggagtcca gcagatactg
    25381 ggaaaacttc gtcgcttccc cgaggcaaat tggcaacatg ccagttgcca gcgggtccag
    25441 cgcaccggta tgcccggcac ggttggcgtt ataaatccgt ttcacttttt gcagcgcatc
    25501 attgctggac ataccctgcg atttatccag caacaaaacg ccgttaatgt cgcgaccgcg
    25561 acgacgagga cgactcatta gtcctccttg ctgtcgtccg ggttaacacg acgttcttcg
    25621 tcatgtttga ccacgctggt caccaggttt gacatgcgca tcccttcaac cagagagttg
    25681 tcgtagaaga aggtcagttc cggcacgata cgcaggcgca tcgctttccc cagcaggctg
    25741 cggatgaaac cagaagcttc ttgcaacgct ttgatgcccg ctttaaccgc gtcttcatct
    25801 ttgtcgttga ggaacgtcac atatactttg gcatacgcca ggtcgcgaga catttcgaca
    25861 ccggaaacgg tggtcatcat gcccaggcga ggatctttaa tttcacgctg caggatgaga
    25921 gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc gaccaaattc tttcgccata
    25981 ataaattctc ctgacaaaaa aggggctgtt agcccctttt taaaattaat ttcaggtgga
    26041 agggctgttc acgttggcct gataagacgc gccagcgtca catcaggcaa tccatgccgg
    26101 atgcggcata aacgccttat cccgcatgga accctaaaaa ccttaagcga tggtacgttg
    26161 gatctcgatg atttcgaata cttcgatcac atcgccagtg cggacgtcgt tgtagttctt
    26221 aacgccgata ccacattcca taccgttacg gacttcgtta acgtcatctt tgaagcggcg
    26281 cagggactcc agctcgcctt cgtagataac cacgttgtca cgcagaacgc ggatcgggtt
    26341 gtgacgttta accacacctt cggtaaccat acagcctgcg atggcaccaa atttcggcga
    26401 tttgaacacg tcacgaactt ccgccagacc gataatctgc tgtttcagtt ccggagacag
    26461 cataccgctc atcgccgctt tcacttcgtc aatcaggtta tagatgacgg agtagtaacg
    26521 cagatccagg ctttccgctt caatcacttt acgtgcagag gcatcagcac gtacgttgaa
    26581 gccaaccagg atggcgttgg acgccgcagc cagggtggcg tcggtttcgg tgataccacc
    26641 tacgccagaa ccgatgatct tcactttaac ttcgtcagta gacagtttca gcaaggagtc
    26701 ggagatcgct tccacagaac cctgtacgtc tgccttcagg acgatgttca cttcgtgaac
    26761 ttcgccttcg gtcatgttgg cgaacatgtt ctcgagttta gatttctgct gacgcgccag
    26821 tttaacttcg cggaatttac cctgacgata cagtgcaact tcacgcgctt tcttctcgtc
    26881 acgtacaacg gtaacttcat cacccgcagc cggtacgccg gacaggccga ggatttccac
    26941 cggaatggac ggacccgctt ccagcacttc ctgacccagt tcgttacgca tcgcacgaac
    27001 acgaccgtat tcgaagccac acagaactat atcgcccttg tgcagagtac cttcacgtac
    27061 cagaacggta gcaaccggac cacgaccttt atcgaggaag gattcgataa ccgcaccgct
    27121 cgccatacct ttacgtaccg ctttcagctc cagaacttcc gcctgcagca ggatagcgtc
    27181 cagcagttca tcgatgccgg tacccgcttt cgcagatacg tgtacgaact ggctttcacc
    27241 gccccactct tccggcagga tgccatactg ggagagttcg ttcttaacac gatccggatc
    27301 ggcttctggt ttatcaatct tgttcactgc aaccaccacc ggtacctgcg ccgctttcgc
    27361 gtgctggatt gcttcgatgg tctgcggcat cacgccgtcg tcggcagcaa caaccaggac
    27421 tacgatgtcc gttgcctgcg caccacgtgc acgcattgaa gtaaacgcgg cgtgccccgg
    27481 ggtgtccagg aaggtgatca tgccgttttc agtttcaacg tggtatgcac caatgtgctg
    27541 ggtaatgccg cccgcttcgc cagaggcaac tttcgttgaa cgaatgtagt ccagcagaga
    27601 ggttttaccg tggtcaacgt gacccatgat ggtcacaacc ggcgcgcgcg gttcagccgc
    27661 agcacccgtg tcacggtcgc tcattaccgc ctcttccagc tcgttttcac gacgcaggat
    27721 aactttgtgg cccatctctt cagcaaccag ctgtgcggtt tcctgatcga taacctggtt
    27781 gatggttgcc attgcgccca gtttcatcat cgctttgatg acctgagagc ctttaaccgc
    27841 catcttgttc gccagttcgc caacggtgat ggtttcgccg atcacaacgt cacggttaac
    27901 ggcctgagct ggcttctgga agccttgctg cagggaagaa cctttacgtt ttccgccttt
    27961 accgccacgt actgctgcgc gtgcttcttc acgatcagct tttgattcag cgtgtttgtt
    28021 gcctttcttc ggacgcgctg ctttcgcgtt acgaccacgg ccacgaccgc cttcgacttc
    28081 acgatcgctt tcgtcttctg cctggcgagc atgttgagaa gtagtgacgt gataatcgct
    28141 ggaatcttca gtcggttccg cgttatcagt ccatttgttt tcttccgcca tacgacgtgc
    28201 ttcttcagca acgcgacgtg cttcttcttc gagtttacga cgcgcttctt cttcagcttt
    28261 acgcttgagc tctgcagctt cctgctcacg gcgggctttt tcagcctggg cgtttttagt
    28321 catatcgtct tgttgattgc tcactttgtc tttttccgca gcttcacgtt tcgcttgttc
    28381 agcagcttca cgcttagctt gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg
    28441 tttagccgat tcttctgcct cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc
    28501 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    28561 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    28621 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    28681 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    28741 agcaaattgc tgtaccaggc cttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    28801 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    28861 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    28921 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    28981 ggccagtttg aatgccaaat cacgatctat cccttcaagg ttcagcagat cgtcagccgg
    29041 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    29101 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    29161 catcggcaca taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc
    29221 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    29281 ctgatgctta gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg
    29341 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc
    29401 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    29461 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    29521 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacacaggc
    29581 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    29641 atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca cttcaatacg
    29701 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    29761 ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    29821 cggcagcata tcttcgcgca ggatcacggc ttcagcattg ttgcccagat ccagagagat
    29881 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    29941 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    30001 cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    30061 caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    30121 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    30181 gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg tcgccagcgc
    30241 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    30301 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    30361 gtggggaaca ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    30421 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    30481 cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag
    30541 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    30601 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    30661 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    30721 cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    30781 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta
    30841 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    30901 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    30961 cactacgaag gccgaagtct ccacagtata tttgaaaaag gactctaagg gaaagtggtt
    31021 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg
    31081 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaag atgcaaatac
    31141 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg
    31201 ccacattttg tcaacgttta ttgctaataa cgtgaatgaa tatccagttc actttcattt
    31261 gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca aaaaacacac
    31321 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    31381 aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
    31441 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
    31501 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
    31561 atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    31621 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    31681 gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca
    31741 acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag
    31801 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    31861 atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    31921 actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt
    31981 tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa
    32041 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta
    32101 tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    32161 ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    32221 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaga
    32281 accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc
    32341 acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    32401 acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg
    32461 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    32521 ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga
    32581 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    32641 gtattggtca attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg
    32701 gttatgccaa aactggcctg ctctcctcct ctgccacttc aggcgtgccg caagtggaga
    32761 atatggaaaa caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    32821 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    32881 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg
    32941 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa
    33001 gatgttgttt tccgttgtgc gatccacgcc atccccaaga acggggctat accagataaa
    33061 catcgggaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt
    33121 cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    33181 ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata
    33241 gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca
    33301 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtattttg
    33361 ctgtaatgct tgcgataaat gcgg
//
LOCUS       DAZUFB010000046        32824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZUFB010000046 DAZUFB010000000
VERSION     DAZUFB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.046"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(128..415)
                     /gene="yebO"
                     /locus_tag="ACHY3M_002448"
     CDS             complement(128..415)
                     /gene="yebO"
                     /locus_tag="ACHY3M_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFS4317352.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(490..633)
                     /gene="mgrB"
                     /locus_tag="ACHY3M_002449"
     CDS             complement(490..633)
                     /gene="mgrB"
                     /locus_tag="ACHY3M_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFS4317353.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     NPW"
     gene            792..1031
                     /gene="yobH"
                     /locus_tag="ACHY3M_002450"
     CDS             792..1031
                     /gene="yobH"
                     /locus_tag="ACHY3M_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFS4317354.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1174..1965)
                     /gene="kdgR"
                     /locus_tag="ACHY3M_002451"
     CDS             complement(1174..1965)
                     /gene="kdgR"
                     /locus_tag="ACHY3M_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFS4317355.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2142..3515
                     /gene="yebQ"
                     /locus_tag="ACHY3M_002452"
     CDS             2142..3515
                     /gene="yebQ"
                     /locus_tag="ACHY3M_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4317356.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASRKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3561..4442)
                     /gene="htpX"
                     /locus_tag="ACHY3M_002453"
     CDS             complement(3561..4442)
                     /gene="htpX"
                     /locus_tag="ACHY3M_002453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFS4317357.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4634..6682)
                     /gene="prc"
                     /locus_tag="ACHY3M_002454"
     CDS             complement(4634..6682)
                     /gene="prc"
                     /locus_tag="ACHY3M_002454"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFS4317358.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6702..7400)
                     /gene="proQ"
                     /locus_tag="ACHY3M_002455"
     CDS             complement(6702..7400)
                     /gene="proQ"
                     /locus_tag="ACHY3M_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFS4317359.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7497..7994)
                     /gene="msrC"
                     /locus_tag="ACHY3M_002456"
     CDS             complement(7497..7994)
                     /gene="msrC"
                     /locus_tag="ACHY3M_002456"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFS4317360.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8124..9407
                     /gene="yebS"
                     /locus_tag="ACHY3M_002457"
     CDS             8124..9407
                     /gene="yebS"
                     /locus_tag="ACHY3M_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFS4317361.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9376..12009
                     /gene="yebT"
                     /locus_tag="ACHY3M_002458"
     CDS             9376..12009
                     /gene="yebT"
                     /locus_tag="ACHY3M_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFS4317362.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12089..13528
                     /gene="rsmF"
                     /locus_tag="ACHY3M_002459"
     CDS             12089..13528
                     /gene="rsmF"
                     /locus_tag="ACHY3M_002459"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFS4317363.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLDD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPT
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13646..13882
                     /gene="yebV"
                     /locus_tag="ACHY3M_002460"
     CDS             13646..13882
                     /gene="yebV"
                     /locus_tag="ACHY3M_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFS4317364.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13987..14178
                     /gene="yebW"
                     /locus_tag="ACHY3M_002461"
     CDS             13987..14178
                     /gene="yebW"
                     /locus_tag="ACHY3M_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFS4317365.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14179..14835)
                     /gene="pphA"
                     /locus_tag="ACHY3M_002462"
     CDS             complement(14179..14835)
                     /gene="pphA"
                     /locus_tag="ACHY3M_002462"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS4317366.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15231..15572)
                     /gene="yebY"
                     /locus_tag="ACHY3M_002463"
     CDS             complement(15231..15572)
                     /gene="yebY"
                     /locus_tag="ACHY3M_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFS4317367.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15585..16457)
                     /gene="copD"
                     /locus_tag="ACHY3M_002464"
     CDS             complement(15585..16457)
                     /gene="copD"
                     /locus_tag="ACHY3M_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFS4317368.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLICATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16461..16835)
                     /gene="yobA"
                     /locus_tag="ACHY3M_002465"
     CDS             complement(16461..16835)
                     /gene="yobA"
                     /locus_tag="ACHY3M_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFS4317369.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16974..17204
                     /gene="holE"
                     /locus_tag="ACHY3M_002466"
     CDS             16974..17204
                     /gene="holE"
                     /locus_tag="ACHY3M_002466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFS4317370.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17306..17962
                     /gene="yobB"
                     /locus_tag="ACHY3M_002467"
     CDS             17306..17962
                     /gene="yobB"
                     /locus_tag="ACHY3M_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFS4317371.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17986..18648
                     /gene="exoX"
                     /locus_tag="ACHY3M_002468"
     CDS             17986..18648
                     /gene="exoX"
                     /locus_tag="ACHY3M_002468"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFS4317372.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18645..20705)
                     /gene="ptrB"
                     /locus_tag="ACHY3M_002469"
     CDS             complement(18645..20705)
                     /gene="ptrB"
                     /locus_tag="ACHY3M_002469"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFS4317373.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSHLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSLVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20914..21573)
                     /gene="yebE"
                     /locus_tag="ACHY3M_002470"
     CDS             complement(20914..21573)
                     /gene="yebE"
                     /locus_tag="ACHY3M_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS4317374.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21900..22256)
                     /gene="yebF"
                     /locus_tag="ACHY3M_002471"
     CDS             complement(21900..22256)
                     /gene="yebF"
                     /locus_tag="ACHY3M_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFS4317375.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22323..22613)
                     /gene="yebG"
                     /locus_tag="ACHY3M_002472"
     CDS             complement(22323..22613)
                     /gene="yebG"
                     /locus_tag="ACHY3M_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFS4317376.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22747..23925
                     /gene="purT"
                     /locus_tag="ACHY3M_002473"
     CDS             22747..23925
                     /gene="purT"
                     /locus_tag="ACHY3M_002473"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFS4317377.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(23981..24622)
                     /gene="kdgA"
                     /locus_tag="ACHY3M_002474"
                     /gene_synonym="eda"
     CDS             complement(23981..24622)
                     /gene="kdgA"
                     /locus_tag="ACHY3M_002474"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4317378.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVHVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24659..26470)
                     /gene="edd"
                     /locus_tag="ACHY3M_002475"
     CDS             complement(24659..26470)
                     /gene="edd"
                     /locus_tag="ACHY3M_002475"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFS4317379.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26705..28180)
                     /gene="zwf"
                     /locus_tag="ACHY3M_002476"
     CDS             complement(26705..28180)
                     /gene="zwf"
                     /locus_tag="ACHY3M_002476"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4317380.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GVMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28518..29387
                     /gene="hexR"
                     /locus_tag="ACHY3M_002477"
                     /gene_synonym="yebK"
     CDS             28518..29387
                     /gene="hexR"
                     /locus_tag="ACHY3M_002477"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFS4317381.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29515..30957
                     /gene="pyk"
                     /locus_tag="ACHY3M_002478"
     CDS             29515..30957
                     /gene="pyk"
                     /locus_tag="ACHY3M_002478"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4317382.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31017..31859)
                     /locus_tag="ACHY3M_002479"
     CDS             complement(31017..31859)
                     /locus_tag="ACHY3M_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4317383.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(31918..32721)
                     /locus_tag="ACHY3M_002480"
     CDS             complement(31918..32721)
                     /locus_tag="ACHY3M_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFS4317384.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWA"
BASE COUNT         8088 a         8369 c         7926 g         8441 t
ORIGIN      
        1 ataatatgat aattatttga agtcagacca gagttttaat ttgaagcagt tgcaccatgg
       61 cagtgcgtat aatcaggatg aattgagtaa cccttcaacg acggggttac tcaagattga
      121 ggaaggatta tcgttcggcg accagacgaa taatatcttc gtcttcaacc gatttttgac
      181 tctcaacggt ctttttatct gctttctcag gttcgtttgc ttcgcacaaa cgacgtaaca
      241 gtgcattttg acgtttttgc tgatccagca acgcctcaag cagttcaatc tgttcgttag
      301 tccgagaact ggcacgattg ataaaaaacc acaagatgag cccgataaga agaaccacca
      361 ccgatacaac caaagacgca atgttcatca cgcctgaatt tacaacttcg ttcatttcac
      421 cacctctgag tagaggcgct attctaccac tgctggagag gaagaaaatc tagtgctgaa
      481 aaaatgatat caccacgggt taaactggtt aatggcacaa attccgctga aaaattgtac
      541 atcctgatcg cacatcatgt tgaatacctg cgcccaaagc agcaagcaag ccaacaccac
      601 gacaaccaga acgacccatc gaaacttttt cactccaatc tccgcttcac ctaccttatg
      661 tcattaaact agcatgcgtt agttaaacag cgcctaacta tgtcgatatt cgtgcttttt
      721 cggaatgagt agcttgcttc agcgcatatt gccgctacga ttaagcgaac ataaaaagag
      781 aagaggttgt aatgcgattc atcattcgca cagttatgct gatagcactg gtatggatag
      841 gattattact cagcggatat ggcgttttga ttggaagcaa agagaatgcc gcaggattag
      901 gtttgcaatg tacctatctg actgccagag gcaccagcac ggtacaatac ttgcacacta
      961 aaagtggttt cttggggata acagattgcc cactgttacg taaaagcaat atcgtggtcg
     1021 ataatggtta agggtaataa ataaccagat tctcactcat gagtaaaata ttcttatagc
     1081 aagcgcgttt aatataacgc accttcaact ttatttttct catgctaaat aaagaacctg
     1141 tctgtttgat attccgacag gttcttactc tcatcagaac ggatagtcgt gataacccat
     1201 ttgggcagaa attttgcgcg ctgcggtatg caacattgcg acatattcct gtaaacgctc
     1261 ttcagagaaa cgcaacgtcg ggaaggagat gctcaaaccg gcaatgacca cgccaaagcg
     1321 atcaaatacc ggtaccgcaa tgcatcgcag cccttcttcc tgctcttcgt tatcttcgcc
     1381 atacccctgc tcgcgcacct ggtccagaac gggtaataac gcttctgtac tggtgatggt
     1441 ccgctcggta ctgcgtttat actccacgcc ctcaagaatt tgcttcactt catcgagatc
     1501 gcgccatgcc agcagtacct taccaatcgc ggtgctgtac agcggattac gacgcccaat
     1561 ccgtgaatac atgcgcaaat tgtacataga gtcaattttg tgaatgtaaa caatactgtc
     1621 ttcgtccagt gcgccgaggt ggatagtttc tttggtcagg cgggagatct cacgcatctg
     1681 gatatctgcg ctacgaatta aatcgacgtt ttgtaacgcg cgagcgccca gttcaaacaa
     1741 tttcagggtc agggaatatt tctccgactc cccttcctgc gccacataac ctaaggtttt
     1801 catggtctgt aaaaagcgat aaacggtgct ttttgacatc atgacgcgct gcgacagctc
     1861 ggttatccct atttcgcgct cttcacccag cgcctgcaaa atgccaaaaa cttttagcac
     1921 ggaagataca gaatcaggct gtttatccag atctgcgtta gccatttatc acctcattgc
     1981 gagtgtttta taaaaatcag aactgttttt tattataatt tcgcaccagg gtggtcgcaa
     2041 tccatctttt gccggttagt tacaattctg cgacatccac cgtgaatatc agtgctagaa
     2101 tcatacccct gttgattatt caccaaagat ataaaattcc tatgccaaaa gttcaggccg
     2161 acggcctgcc attgccccag cgatacggtg cgatattaac cattgtgatt ggcatttcga
     2221 tggctgtcct tgacggcgca atcgccaacg tcgccctgcc aacaatcgcc acggaccttc
     2281 atgccacgcc agccagttcc atctgggtag tgaacgccta tcaaatcgcc attgtcatct
     2341 ccctgctctc gttttcgttt ctgggcgata tgtttggcta tcgacgtatt tataaatgcg
     2401 gtctggtcgt ttttctgttg tcttcactgt tctgcgccct ttctgattcg ctgcaaatgc
     2461 tcacccttgc gcgtgtcata caaggtttcg gcggtgcagc gttgatgagc gttaataccg
     2521 cacttatccg cctgatctat ccacaacgtt ttctggggag agggatgggc ataaactcgt
     2581 ttattgttgc cgtttcttct gctgccgggc cgacaattgc tgcagcaatc ctctccatcg
     2641 catccaggaa atggttattt ttaatcaacg taccgttagg tattatcgcc ctgcttctgg
     2701 cgatgcgttt tctgccaccc aatggttctc gcgccagtaa accccgtttc gacctgccca
     2761 gcgccgtgat gaacgcgtta accttcggcc tgcttatcac tgcgttgagt ggtttcgctc
     2821 aggggcaatc gctgacgtta attgctgcgg aactggtggt aatggttgtt gttggtattt
     2881 tctttattcg ccgccagctt tctcttcccg taccgctgct accggtggat ttactgcgta
     2941 tcccgctgtt ttcactttct atttgcacat ctgtttgctc tttctgcgca caaatgctgg
     3001 caatggtttc cctgcccttt tacctgcaaa ccgtgctcgg gcgtagtgaa gtcgaaacag
     3061 gtttacttct gacaccgtgg ccgttagcaa cgatggtgat ggctccgctg gcaggctatt
     3121 tgattgaacg cgtacatgca ggattgctgg gtgctttagg gttgttcatc atggctgcgg
     3181 ggcttttttc cctggttctg ctgcccgcgt cacctgcgga tatcaatatt atctggccga
     3241 tgatcttatg tggcgctgga tttggcttat tccagtcacc caataaccac accattatta
     3301 cctccgctcc gcgcgaacgt agcggtgggg ccagtggcat gttaggaacg gctcgtctac
     3361 tgggtcagag tagtggcgcg gcgctggtgg cgctgatgct aaatcagttc ggtgataatg
     3421 gtacgcacgt ttcgctgatg gctgcggcta ttctggcagt gattgctgcc tgtgtgagtg
     3481 gtttacgtat cactcagcca cgatccaggg cataaaaaaa gcgcgtcgat caggacgcgc
     3541 cttttagtat ttacttcata ttacttcagg tattcacccg tacgcagagc ttcaattcgt
     3601 ttatccagcg gcgggtgagt catgaacaac tcactgagcg atttcgactt accgttaatg
     3661 cagaaagcca tcatgctggt tgcttcttgc ggttcatagc tggttttcag gcgctgcagc
     3721 gcggcaatca ttttctcgcg accaaccagt tttgccgaac cggcatcagc atggaattca
     3781 cgatgacgcg agaaccacat ggtgataatg ctcgccagaa taccaaacac cagttccaga
     3841 accgttgcaa ccgcaaagta gatcagcggg ttgccgttgc tctcttcacc ttcatcacga
     3901 tttccgccca taaaacccgc ggcaagctgc gccagaatac gggaaataaa gataacgaag
     3961 gtgttcacca cgccctgaat cagcgtcatg gtaaccatat caccattggc gatgtgactg
     4021 atttcgtgag caattaccgc ctcggcttca tccgggctca tgttctgcag caaaccggtg
     4081 ctgacagcaa ccagagaggc atcacggcgc gcaccggttg caaaagcgtt gatgtccggc
     4141 gcatggtaga tagccacttg cggcatagcg atccctgcct gacgagcctg ggttgctaca
     4201 gtattgacca gccaacgttc cctttcgtta cgcggttgct cgatcacttc cccgccaaca
     4261 gatcgtaatg ccatccattt ggacatcaga agcgaaacga aggaaccacc aaaaccgaac
     4321 agcaaggcca tgatcatcag cccctgaacg ctgctcgact gtatccctgt caggctcagt
     4381 accagcccga aaacgaccat tacggccagg ttcgttagca ggaagagcgc gattcgcatc
     4441 ataattttct ttttacctca gtttaacaaa acgcaatatg cgatacccac atcgtatggg
     4501 ttacgcgact attttcaagt ctggatagtg cgtaagtcac cagaaagaca caactttaca
     4561 ttttgtagca tctgatttac ggcatcttgc cgctgttaaa aaatcaggca caatttcttg
     4621 tgcctgattg atattacttg acgggagcgg gttgttccgc gggtctggct ttttcaagct
     4681 tcgccagatc gagtgcgata ttcaccgtct catccagata aggatccggc tcctggtaat
     4741 ctttcggtag atcatccagc ttcttcaact ccggtttacc ttcgcgttta aagcgttcgt
     4801 tcaaacgcgc cagacgcgtc gcatcatctt cattattctc tttctcacgc acagcgtagt
     4861 tcagagaaac gatattgcgc ttgtccttca tagcgttgaa gcgcgcgata tccttcataa
     4921 tgttctggaa ctcaggatct ttcgcgatac gcgcattatg ttccttcagc agctccggcc
     4981 caaaggccgt taaatcgcct gatttcacat aagtcgcggc atcaatgcta tcccacggca
     5041 gcgcgttatc ttcgaatttc tcacccgttt ccgtttcttc attacccgtc ggcatgatga
     5101 tgtctggcgt tacgccttta cgttgcgtac tgccgccgtt aacgcgatag aatttctgga
     5161 tcgtgtactg cacagaaccc agcgctggcc attcaggacg taacatctga tcgtaaatac
     5221 ggttcaatga acggtattgc tgaacggtgc ctttaccaaa cgtcggttca cccacaacca
     5281 gcgcacgacc gtaatcctgc attgccgcgg caaagatttc tgaagccgaa gcactgaaac
     5341 ggtcaaccag taccaccagc gggcctttat agaacacctg cccgtcggta tcgctgtctt
     5401 cacgaacctt gccgttgtta tcgcggacct gaacaatggg acccgcagga ataaacagac
     5461 ctgagagcga taccgcttcg gtcaacgccc caccgccatt gctacgcagg tcgatgatca
     5521 cactgctgac attctgtttt tccagtttct gcagttgaac tttgacatcg tctgtcaaac
     5581 ccacatagaa gcccggaata tccagcacgc cgactttctc tttaccgacg gtcttcaccg
     5641 acattttaac cgcgcggtct tcgagacgaa tacgttcacg ggtcaatgtt acagtgcggg
     5701 tcttggtccc tttaccagca ggtaaaattt ccagacgaac tttactgccc ttcggccctt
     5761 taattaaggc aaccacatca tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg
     5821 tttgaccaac accgacaatt ttgtcaccaa cgctgatagc tttactcttc gctgccggac
     5881 cacctgccac catcgaattg ataacggtgt agtcatcatc catttgcagc actgcgccaa
     5941 taccttccag cgacaaactc atttcagtgt tgaactgttc ggtattacgc ggggaaagat
     6001 agttggtatg cgggtcgatt tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt
     6061 cgctgttggt ttgcgccaga cgacgaatgg caaatttgta gcggcgtgtc agggtttcac
     6121 gaatttcttt atccgttttt cctgccagct tcaggcttaa ctcgtcgaat ttgactttac
     6181 tgtcccacag cgcgttcaac tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa
     6241 ggttataagt gtcgttgccg gtgaaatcca tcggcttttc cagtaccgac aaagcgtact
     6301 ggtaacgctc aaaacggcgc ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt
     6361 tgcctgaacg cagttcatcg cctaactcgg ttttcttttt cgcgaactgt tcaacatcgc
     6421 ttgccagcag cacgttgtgg ctgtaatcga gcagattcag gtagcggtca aagattttgg
     6481 ccgaaaatgc ctgatcgagg tcgaactggc gataatgaga acgggtgaag cgcgacgtta
     6541 cgcgctcact taccgtcgca tgctgcgtct cttcctttaa taccggaatt tgatcagcac
     6601 gcgtgatatc ttctacagcg aaggtctggc ctgctattgc aagcaggcca gctaacgcgg
     6661 taagcctaaa aaacatgttc atgcctggcc cggcctccgt ttcagaacac caggtgttct
     6721 gcgcgcacaa tcaaagacat acccgaattc agctggacgc ggacgccgtc tttggtgatt
     6781 tctaatacgg tggcatccat cgcgttttga cccgctttca ccttcagggc ttgtccgaca
     6841 gtcagagctg aaatgtcaga aactggggtg tgctgttctt cgcgaggtgc ttttactgtt
     6901 tttggcgctt tctctaccgg cttttgcgca cgaggtttac gttcagcgcc ttctttgcgg
     6961 cgtggcgtgg tcggacgtgg cttgcgttcg cggcgcggtg cgtcttcttt ctcaccagca
     7021 gctgcggcag cttcgcgttt tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct
     7081 ttcgcttctt caagctgctt gcgagcatgc tctacatgtt gctcgtccag ctcaccgcat
     7141 gggttgccgt caagatcgac acgcgttgcg ccgggtttaa caccgtaaag ataacgccag
     7201 ctcgaagtgt agagacgtaa agcggatcgt aattgcgttt tgctcaggtt catttccccg
     7261 gcaacacgat cgaccaaatc ctgaaaaata ccgattttca gcggacgcgc ttcaccttcc
     7321 gcactgaaac agtggggaaa acgttcggcc agaaacgcga ttacttcttt actgctattc
     7381 aacttaggtt gattttccat gaaatttcct gattacaacg gacgtagcca acaagcgcag
     7441 gcatgaacag gcgtcattat aatgacgcta ccagtaaatg ctacgttatc cgttgattat
     7501 cctgcgacgc tcgcaaagaa ttttttgtaa tccgtcgttg caagcacttt ttcaagctgt
     7561 gccacaagct gacgtaagcc ttgctcgtcc tcgtctgtaa agcgaccgaa gacggtacta
     7621 tcgatgtcaa gaacaccaat aatctgattt ttcaccacca gcggcagaac aatttcagaa
     7681 ttactcgccg catcacaggc aatatgcccg tcaaacgcgt gaacatcctc gatacgctgc
     7741 acttgattgc gggcaaccgc agtgccgcac accccgcgcc cgacgggtat ccggacacag
     7801 gcaattttgc cctgaaatgg tcctagtacc agtgtatcgt cctcaagcaa ataaaaacct
     7861 gcccagttta cgtcagtgag acgctcgtat aacaacgcac tggtgttcgc aagcgttgcc
     7921 agaaaactgg tttctcccgc catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat
     7981 tctgttttgt tcattatata atcacttggt tgtcttacct ggaactgcca gcctattaaa
     8041 ataagcatta aatgcgttaa tgctcaagat cattccaatc atgggttaat attaatgtta
     8101 attcttatta catttggcac gtcatggctc ttaacacacc acaaattacg ccgacaaaaa
     8161 agataacagt gagggcaatc ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc
     8221 aatgtgacat gctgtttagc ctgcccgaga taaattctca tcagagtgcc tattgtccgc
     8281 gctgtcaggc aaaaattcgc gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg
     8341 ctttcaccat gctgttgctg atgccgtttg cctggggcga accgctgttg catatctggc
     8401 tgttaggtat tcgtatcgac gccaacgtca tgcaaggcat ctggcaaatg accaaacagg
     8461 gcgatacgat aacgggggcg atggtctttt tctgcgttat tggtgccccc ctcattctgg
     8521 tgtcctccat agcatattta tggtttggta accgactggg aatgaattta cgtccggtac
     8581 tgctgatgct tgagcgtctt aaagagtggg taatgctgga tatctacctg gtcggcattg
     8641 gcgttgcttc tataaaggta caggattatg cccatatcca ggctggtgtc ggcttgttct
     8701 cttttgtggc gttggtgatt ttaacgacgg tgacgttgtc acatcttaat gttgaggagc
     8761 tgtgggagcg atattatccg cagcgccccg ctacgcgtag ggacgagaaa cttcgtgtct
     8821 gtcttgggtg ccattttacc ggctatcctg atcagcgtgg tcgctgcccg cgttgccata
     8881 tcccgctacg cctgcgtcgc cgtcatagtc tgcaaaaatg ctgggcggcg ctgttagcgt
     8941 caatcgtttt gttgttacca gccaacctat tgcctatttc tatcatttat ctgaacggag
     9001 gacggcagga agatacaatt ctttccggaa ttatgtcgct ggcaagtagc aacattgcgg
     9061 ttgcgggaat cgtgtttatc gccagtattc tggtaccgtt tactaaagtg atcgtcatgt
     9121 tcactttact gttgagcatt cattttaaat gccagcaagg tttacgcaca cgcattctgt
     9181 tactgcggat ggtgacctgg attggacgct ggtcgatgct cgacctgttt gtcatatctt
     9241 taaccatgtc gctgattaat cgcgatcaga tcctcgcttt tactatggga ccggctgcgt
     9301 tttattttgg cgcagccgta attttgacta ttcttgctgt ggaatggctg gacagccgct
     9361 tactttggga tgcacatgag tcaggaaacg cccgcttcga cgactgaagc gcagattaaa
     9421 aataaacgcc gtatctcacc tttctggctg ctgcctttca tcgcgctaat gattgccggt
     9481 tggctgattt gggacagtta tcaggaccgg ggtaataccg tcaccatcga ctttatgtcg
     9541 gcggatggta ttgttccggg ccgtacgcct gttcgttatc agggcgttga agtcggaaca
     9601 gtgcaggata tcagcctcag cgacgatctt cgtaagattg aagtcaaggt cagcatcaag
     9661 tccgatatga aagatgcgct gcgcgaagag actcaattct ggctggtgac gccaaaagca
     9721 tcgttggcag gtgtgtccgg gctggacgcc ctcgtcggtg ggaactatat cggcatgatg
     9781 ccgggtaaag gtaaagagca ggatcacttt gtcgcactcg atacccaacc gaagtatcgg
     9841 ctggataacg gcgatctgat gatccaccta caagctcccg atctcggttc gctgaacagc
     9901 ggttcattgg tctatttccg caagatcccg gtgggaaaag tctacgacta tgccatcaat
     9961 cccaacaagc aaggcgtggt gattgatgtc ctgatcgagc ggcgttttac cgatctggtg
    10021 aaaaaaggta gccgtttctg gaacgtttcc ggcgttgatg ccaacgtcag tgtcagtggc
    10081 gcgaaggtga aactggaaag tctggcggca ctggttaacg gtgcgattgc cttcgattca
    10141 cctgaagagt cgaaacctgc cgaggcggaa gatacctttg gtctgtatga agatctggcc
    10201 cacagccagc gtggcgtaat aataaaactg gaactgccga gtggggccgg attgaccgcc
    10261 gactcgacgc cgttaatgta tcaggggctg gaagtcggac agctaactaa actggattta
    10321 aatcctggtg gtaaagtcac tggggaaatg acagttgatc ccagcgtcgt taccctgctt
    10381 cgtgaaaata cccgcatcga attacgtaat ccgaaattat cccttagcga tgccaatctc
    10441 agcgccctgc tgactggcaa aaccttcgag ttggtacccg gcgatggcga gccacgcaaa
    10501 gagttcgttg ttgtgccagg cgaaaaagca ctgctgcatg aacctgatgt tctgacgctg
    10561 accctgaccg cgccggaaag ttacggtatt gatgctggtc agccgctcat tcttcacggc
    10621 gtgcaggtag gccaggttat cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc
    10681 gccatcgagc ctcagcatcg agaactggta aaaggcgata gcaaatttgt cgtcaacagc
    10741 cgtgtcgacg tgaaggtggg gctggatggc gttgagtttc tcggtgccag cgcctcagaa
    10801 tggattaacg gcgggatacg tattctgccg ggcgataaag gcgagatgaa agccagctat
    10861 ccactgtatg ccaatctgga aaaagcgctg gagaacagcc ttagcgattt acccaccaca
    10921 accgtgagtt tgagtgcaga gacgctgccg gatgtgcagg caggatcggt agtgctctac
    10981 cgtaaatttg aagttggtga agttattacc gtccgtccgc gagctaacgc gtttgatatc
    11041 gatctgcata ttaagccgga gtatcgcaac cttctgacca gcaatagcgt gttctgggca
    11101 gaaggcgggg cgaaagttca gctgaatggt agtggcctga ccgtacaggc atccccgctc
    11161 tccagagcat taaagggagc cattagcttc gataacctca gcggtgccag cgccagtcag
    11221 cgtaaaggcg acaaacgaat tctgtatgct tccgaaacag cggcccgtgc ggttggtggg
    11281 cagattacgc ttcacgcttt cgatgccgga aaactggcgg tcgggatgcc aattcgctat
    11341 ctcggtattg atatcgggca aatccagacg ctggatctga ttaccgcgcg caatgaagta
    11401 caggcaaagg cggtgctcta tccggagtat gtccagacct tcgcccgcgg cggtacgcgc
    11461 ttctcggtgg tcacaccgca aatttcagcc gcgggcgttg agcatctaga tactatcctc
    11521 cagccgtata tcaacgtcga accaggccgg ggcaatcctc gccgcgactt tgaattacaa
    11581 gaggccacca ttactgattc gcgttacctg gatggcttaa gcattattgt tgaagcgccg
    11641 gaagccggtt cgttaggtat tggtacgcct gtgctgttcc gtggtctgga agtcggtacg
    11701 gttacaggaa tgacgctggg gacattgtca gatcgcgtga tgattgcgat gcgcatcagt
    11761 aaacgctatc aacacctggt gcgtaacaat tccgtcttct ggttggcatc gggttacagt
    11821 ctggactttg gtctgacggg cggcgtagtg aaaaccggca cctttaacca gtttatccgt
    11881 ggcggcatcg ccttcgccac gcctccgggt acgccactgg caccgaaagc ccaggaaggc
    11941 aaacacttcc tgttgcagga aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt
    12001 cccaaataat gcccactgct ccggcgtgcc tgcgccggag cgtttatgct aaactgcgcg
    12061 cctgtttttt tgccagtggt acatgctcgt ggcccaacac accgtttatt tcccggacgc
    12121 ctttctgaca caaatgcgcg aagcgatgcc ttcgacgctc tcatttgatg attttcttgc
    12181 cgcctgtcag cgcccgttgc gccgcagcat tcgcgttaat acgctgaaaa tctccgttgc
    12241 tgatttcctg caattaaccg ctccttatgg ctggacgctt acgccaattc cgtggtgtga
    12301 agaaggtttc tggattgaac gcgacaatga agatgcattg ccattgggta gtaccgccga
    12361 gcatttaagc ggcctgtttt atattcagga agccagttca atgttgcccg ttgccgcctt
    12421 gtttgctgac gataatgcac cacagcgggt gatggatgtc gcagctgcgc caggctccaa
    12481 aacgacgcaa attgccgcgc ggatgaataa cgaaggggca atccttgcca atgagttttc
    12541 cgccagtcgg gtaaaagtgt tacatgccaa tatcagccgc tgtggcatca gtaatgttgc
    12601 gctcacacat tttgatggcc gcgtgtttgg tgcggcagtg ccagaaatgt tcgatgccat
    12661 tttgctggac gctccctgct ctggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa
    12721 aaactggtca ccagaaagca atcaggaaat cgcagctaca caacgggagc ttatcgacag
    12781 cgcctttcat gcattacgtc ctggtggtac gctggtttac tcgacctgta ccttaaacca
    12841 ggaagaaaac gaagccgttt gcctgtggct gaaagagact taccccgacg cagtagagtt
    12901 tttaccactt gacgatctct tccctggtgc aaacaaagcg ctgaccgaag aaggcttttt
    12961 gcatgttttc ccacaaattt acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac
    13021 tcaggcgatt cccgccttac ccgcccccaa atacaaagtc ggtaattttc cgttcagccc
    13081 ggtgaaagat cgcgaagctg gacaaattcg tcaggcgact gcaggtgttg gcttaaactg
    13141 ggatgaaaac ctgcgcctct ggcagcgtga caaagaactg tggttgttcc cggtgggcat
    13201 tgaagccctg atcggtaaag tccgattttc tcggttgggg attaaacttg ccgagacgca
    13261 caacaaaggt tatcgctggc agcatgaagc ggttattgct cttgcctcac ccgataatat
    13321 gaatgcattc gagctgacac cgcaggaagc ggaggagtgg tatcgcgggc gcgatgttta
    13381 cccgcaagcc gcgccagtgg cggatgatgt attggttact ttccagcatc aaccgactgg
    13441 tttagccaaa cggattggtt cgcgattgaa aaacagctat ccgcgtgaac tggtgcgcga
    13501 tgggaaactt tttaccggta acgcctgaca gctcacaaaa aaagcgcact ttttgactgg
    13561 cacattcggc tgcctcaact aggctgaaaa atggtgcgat cggactggtc gtaccacaat
    13621 cggcagctaa atggagagca cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat
    13681 cgacgacggc gaattacacg gtgaatcgcc gggtgatcga acgttaacca ttccttgtaa
    13741 atctgacccc gatttatgta tgcaactcga tgcctgggat gctgaaacca gtatccctgc
    13801 cctgctaaat ggcgaacact ctgtccttta ccgtacccgt tacgatcaac aatctgatgc
    13861 ctggattatg cgtcttgcct gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc
    13921 tggttattcc cccgttgtaa aatctctcct aaacttaacg gtacggcacc acacttcggg
    13981 gatgaaatgt tcgcgctggt actttttgtt tgctacctgg atggcggttg tgaagatatt
    14041 gttgtggatg tctacaacac ggaacagcag tgtctttatt ctatgagcga tcaacggatc
    14101 cgccatggcg gttgttttcc gattgaggat tttatagatg gtttctggcg accagcacag
    14161 gagtatggtg atttttaatt attgcaattg cacaagagtc agttcgcccc caaagacagc
    14221 accggtatca atataatgca ggttgccaat atccacgcga tgtcgcaacg gtgtatgacc
    14281 aaaccagaaa tgatcagcac ctgtaattcc ctgccctttt tggcgttcac ctaatcgcga
    14341 gcggctccac aagacctgat gcaaatcaac gtccttttgc cattcataaa catcatctgg
    14401 ataatcggca tgagcaataa catgtttgcc ggtacgactg tgtacttcaa gaataaaggg
    14461 caaatgctga catttttcca gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc
    14521 aataaaccag tcgccgccat tcatcaacca caaagacatc tgctgggatg ccagcgcatc
    14581 catcgccatc tgttcatgat tgcctcttac cgcacaaacc caatgttgtt ccagtaactg
    14641 cagacaacgt aaactttgcg gcccacgatc gataacgtct cctactgaga taagtaaatc
    14701 tcgccacgga tcaaaacgac aatgccataa tttgcggcgc aactgctcaa gacaaccgtg
    14761 tatatcgcca gaaagccaga tatgtcgcca ttgatgaccc gcaattctct gataaacggg
    14821 cgcaggctgt ttcatcaata ttttcctccc gcgctaaaga tcacataatc ttaacaagaa
    14881 tgttaaaaaa cgctggactc agacagtaga gtgtgtgtta tggttgacta taaagtcagc
    14941 gaaggaaatg cttctggctt ttaacagata aaaagagacc gaacacgatt cctgtattcg
    15001 gtccagggaa atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct
    15061 tagttgcctt gccctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa
    15121 tggtcaataa aaagcgtggt ggtcatcagc tgaaatgtta aaaaccgccc gttctggtga
    15181 aagaactgag gcggtttttt tattggaaat caaaaggcta ttttaggtaa ttaacagagt
    15241 ttttcagctc gttctataaa cggtgccaga ctcatttttt cgccgggatt gttaggatca
    15301 tcaatctgaa tcaccgaaat gggttgggct ttagtcttcc cactggcaac ttccttttgt
    15361 gcgatatcgt ttaaaggata ctgcacgagg gtacttgggt taatgacata caaagcatta
    15421 cccggtctgc aagtcagcat cacctcttcg cgattaaacg cccatttgtc tttacccact
    15481 tcaaaacgac tgacggtaat cacctgcggt gcagccagcg ccgctgcaga actggtgagt
    15541 aacagaaacg tcagaatact ttttttcatc attttcgtaa tccatcaaaa aggttcgaga
    15601 gtcgcaagca ggctgatgat caccagcact accgcaccta tcgcccattc gagtttagtc
    15661 atccactcaa accagggtgc cgcacgatct tcatcttgcc tcatgcgcgg tacaagaaca
    15721 taacgattag ccagcgcaat gaccaccata atcatcacca ggatggcttt gagcaataag
    15781 agttggcccc agtaagtcgt aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc
    15841 acgccgcttg ccagtacgcc gatcaccgca aaatgcccgc accaggaaaa acgcatcagc
    15901 gcctgaatgg cctggtgtcg ccaacggcct ttgatgagtt gcatacacca gagcaccggc
    15961 agtaatcccc caaaccacgc cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg
    16021 attttcgccg ttaccccttc attcagtgtc gcatgcccga ttcccgccag cagaataaat
    16081 tgcgcggtag tgagcataaa cagcaaacgt ggcatattcc gcggttgcat aagggcgacg
    16141 atcaacgtta cgagggcgag aacaatttgc cataaccaga cgccaccaaa ctgcgtttgc
    16201 aaaacagcct gccaaatgtt tggcgaaaat acatctgccc atcccgttcc catcaacccg
    16261 ccctgaacgg cgagcattgc agtggcacag atcaaactcc agacggcagc gtgctgttgt
    16321 aaacgcagaa aacgcttcgc cagcaagcga cgaatcgtta agggggccag ccaggcaccg
    16381 tacattgcga agccaaagac cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc
    16441 caggtaaacg ccagcatgat ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc
    16501 ccgtccaccg aaacaacatg ccagtcaacg atgtaggttc cgggtttcag cgaatcggcc
    16561 agtggaacaa tcagttgctt ttggtcctgc tcatttcgct tcgccggtaa tgttttaata
    16621 ttttcgtttt ttggccccgt gatttttgca ccactgaatc ctgtttcaac accttccgag
    16681 aagtttaaag tgattgcctg cggtgcagct gtcacttgcg cgtttgccgc aggatactga
    16741 tgcgttaaat gcgcatgtgc ccagacggaa ggtgtcacca acgaagtggt caggattgcc
    16801 agcgcgtagc gaagggagcg tgcagttgaa gtcatattat ctattccttt ttgtaataac
    16861 ttttttacag agcataacct tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc
    16921 catcctggct cactcttagt aacttttgcc cgcgaatgat gaggagatta agaatgctga
    16981 agaatctggc taaactggat caaacagaaa tggataaagt gaatgtcgat ttggcggcgg
    17041 ccggggtggc atttaaagaa cgctacaata tgccggtgat cgctgaagcg gttgaacgtg
    17101 aacagcctga acatttgcgc agctggtttc gcgagcggct tattgcccac cgtttggctt
    17161 cggtcaatct gtcacgttta ccttacgagc ccaaacttaa ataaaactta tacagagtta
    17221 cactttctta cataacgcct gctaaattat gagtattttc taaaccgcac ccataatttg
    17281 cagtcatttt gaaaaggaag tcattatgtc gttctggaaa gttgcagcag cgcaatatga
    17341 gcctcgtaaa acctcactca ccgaacaggt agctcatcat ctggagtttg tgagggccgc
    17401 cgccaggcaa caatgtcagc tgttagtttt tccatcgctt tctttactgg ggtgtgatta
    17461 ttcgcgacgt gcccttcctg ccccacccga tctctcactg ttagacccgc tttgttatgc
    17521 cgcaacgacc tggcgaatga ccatcattgc cggccttccc gttgaatata acgatcgctt
    17581 tattcgtgga attgcggtgt tcgcaccgtg gcgaaaaacg cctgggatct accatcagag
    17641 tcatggtgca tgtctgggtc gtcgttccag aaccatcacg gtggtcgatg aacaaccgca
    17701 aggtatggat atggacccga cctgttcact gtttactaca gggcaatgtc tgggagaacc
    17761 cgacctgctg gcgtcggccc gtcgcttaca atttttttca catcagtact cgattgccgt
    17821 gctgatggca aatgcccgtg gtaacagtgc actatgggat gaatatggtc gtctcatcgt
    17881 tcgcgccgat cgtggttcat tattgttagt cggtcagcgt tcatcacagg gttggcaagg
    17941 cgatatcatt ccattacgct aggctttttc ggcctggagc atgccatgtt gcgcattatc
    18001 gatacagaaa cctgcggttt gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt
    18061 gacggaaaaa tcgtcaaccc catgagccac ctggtgcgcc ccgatcgtcc tattagtcca
    18121 caagcgatgg cgattcatcg catcaccgaa gccatggtcg ccgataaacc gtggattgaa
    18181 gatgtgatcc cacactatta cggtagtgaa tggtatgtcg cgcataacgc cagctttgac
    18241 cgccgcgtac tgcctgagat gcccggtgag tggatttgca ctatgaaact ggcccgtcgt
    18301 ttgtggcctg ggatcaagta cagcaatatg gcgttatata aaacacgcaa gctcaatgta
    18361 cagacgccgc cgggcctgca tcatcaccgc gcgttgtatg actgttatat caccgccgcg
    18421 ttgcttatcg atattatgaa cacctccggc tggacggcag aacagatggc cgatatcacc
    18481 ggacgtccgt cgttgatgac gaccttcacc tttggcaaat accgtggcaa agcggtttcc
    18541 gacgttgccg aacgcgatcc gggctatctg cgctggttat ttaataacct ggacagcatg
    18601 agcccggagc tgcgtttaac actgaaacat tatctggaaa atacttagtc cgcaggcgta
    18661 gcgggtaatg ttccctgcgc cagcgcgacc agaaaagcat attccatcgc tacgccttcg
    18721 tacgatttaa agcgaccaga tttaccgcca tggcctgagt ccatgtcggt acagagcaat
    18781 aaaagatggt catcggtttt cagctcgcgc aatttagcga cccattttgc cggttcccaa
    18841 tattgcacca gagaatcgtg caaaccggtc gttaccagta aatgcggata agcctgtgcg
    18901 gtgacgttgt catacgggct gtagcttttc atgtactcgt aatattgcgg atcctgcgga
    18961 ttaccccact cttcaaactc accagtggta agaggaattg attcatcaag catcgttgtt
    19021 acaacatcaa caaacggtac ctgggcgata acgccgtgga ataattccgg gcgttgatta
    19081 attgcaacgc ccatcaacat gccccccgca ctcccgccca tcgcataaca aagcgaagga
    19141 gagccatagc ccagttttaa caatgcatcg caggcatcaa gataatcatt aaacgtattt
    19201 ttcttcttca gaaattttcc gtcttcgtac cattgttgcc ccagctcacc accgccgcga
    19261 acatggacaa tggcgtagac aaagccacga tctaacaaac tcaagtggct aaaactgaaa
    19321 tcggcatcaa tacttgcgcc gtaagaacca tagccataca ccagcaacgg gttgtgtcct
    19381 ttgcgaaaat gtttgcgatg gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg
    19441 actatccaca ggtgttcact gcggtaattc gccgcatcaa aaccgggcac ttctgtttgt
    19501 tttaatacac gacgctcacc ggtatccata tccagttcaa acaaagtgtc tggtgtggtc
    19561 atggaagaat aaccataacg caatcgcgcg gtttcaggtt ctggattgta ggcaatccag
    19621 gtcacatagg ctggatcatc aaaggcaata ccaatgactt cccgggtctt gcggttaatt
    19681 tggcgcaaac tggttaaccc gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc
    19741 gtaaaccctt ccagcatgat gttttcgcgt ggcggaatta actcttccca ctgttgctca
    19801 tcacgcatac gggtacggta taagccaaag tttttgccgt ggcggttgga acgtagataa
    19861 aaacgatgct ggtagtgatc aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca
    19921 aacggctcgg catcggccat ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg
    19981 gccaaatgaa tgactacata gtgcttcgag gtcgttttat gcaggctgac gtaataggta
    20041 tcgtcttttt cttcgtagat cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc
    20101 cagacctgat aaggcagcag cgtcaccgga tgcttgcgaa catagtagaa agtccaggag
    20161 tcatttgccc agacaaagct gggttcaacg ttatccagca gttccgggta ccagttacca
    20221 gtttccagat tacgaaaacg aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc
    20281 gccataatgg tgttatcggg cgtaatcgcc attccgccca tcgaataaaa ctcactatga
    20341 gctgcgcgct tattggcatc gagcaatgtt tcccactcat cccactcttc actgaatgcc
    20401 gattgacgct ggtagatagc atattcacag cctggttcat aaatatgccg atagcggtag
    20461 ccatttttga tgtagggcgc agaaacttct cgttgcggaa tgcggtcgat gatttccttt
    20521 aagatgcgat cctgcaaggc ttgttgtgag gccatcaccc gatgaccgta actattttct
    20581 tgttgcaggt agtccaggac ttctggctga gaacgcgtat cgtcccgcag ccagtagtaa
    20641 ttatcgatgc gcgtatcgcc atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt
    20701 agcatgttat tgttctttct ggttgaaaca tcttataagg gtggcaaaac tcaccgggga
    20761 tgcaagcgaa acaggggagt cattgcttag atgatgacag gtaatggcgc ggatatcgaa
    20821 tgttatgcag acagagaaaa tcagcctgtt caaatggctg tgcgattctg gatagcccga
    20881 aatagtcaac ttcaggctat ccagagagcg gaattattcc gccaaagtgc gtttttgctg
    20941 ttcgagatcg cgttcaatgc cgtcacgaac atcctggggg attttcagcg cgtcacccag
    21001 tgcattcagg taactgcgtt ccataaaatg gtcaatatca atagccgcgc aactcaggaa
    21061 atagatttcc agcgcctctt cttcattgcg gaccccggta gccaggcgtt gtggatccag
    21121 cggttgttcg attgcctgct caatgagtac acgcccctgc tcttccacgc cggcttcacg
    21181 caattgctgg tcgattgccg cacgttcttt ggcatcaata tgaccatcac ttttagcggc
    21241 aaagaccagc gcaaggatca aacgttctgt acgctcatcc agcggcgtac tttgcgcgcc
    21301 aaactgcggt tcgtcctgat gcgccgcgcg aattttatct ttgtatttat tccacagcac
    21361 cgtacccgct accgctccgc cgccaaccag taacgcgttg gtgccatatt tcgtaagaag
    21421 tttacgtgct gatttatttg cgaccagcag cccagccagt ccgcctaatg cgcctggcac
    21481 taacagtttg cccaatccct gatccgcaga cgaggaggta gaagaactgc tttgcccaag
    21541 cagggattgc agttgattta accagttagc catatttgct cctcaataac catttttagt
    21601 gcctgtagca tagcggatga ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa
    21661 taagcggggc ggtaggccgt tattcaaaga aattcgcgcc aggtaaggta tttactgtat
    21721 atcaggtgat aagggtattt taggtgaaag tagtttgtcg ttttgagtgg cattgtttga
    21781 tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc ctacgattgt ggtgcgttct
    21841 ggggcggaca aggcactcgc gccgcatccg ccaacggtca atcgaattat cagaagccat
    21901 taacgccgct gatattccgc cattcccgct ttgcagtcca cgctgacctg gtaatgaata
    21961 tcggcacttt taccacgcac ggttagcggt actgaccatt tatcatcttt accctgaatg
    22021 tcctgcaaac tgacccaagc cacgggatcg gcctgaccga caattttttg atcatctgcc
    22081 caacgcgcca cgcgattttg ttgataatca cgttttacgc tcgcggcaat tccggcagca
    22141 tccagacctt cacactttgg gaaagtgacc gacttgctgg tttcattatt ggcagcgaaa
    22201 actgatgcgc aggcagaaac caacaacagc cctaaaaacg cccctctttt tttcatgttt
    22261 ttctccatag cacaatgatt caggagaaag catggtacaa attgtcagga gcgcaagttg
    22321 cttcaggccg cgtgtgaggc atcttcctct tcggattcag caccgacgac ctgtggggac
    22381 ggtaatttgc cggttttcag aatggtgctc agcacatctt tttgttctgc cagccatagc
    22441 gaaagggctt cacgttgctc gtcttccatt tgcactggag aatttgtcag ccaggtgtca
    22501 agcagatccg ccgtatcaag cattttatca taagcatcgg cttccttttt gctggtaaac
    22561 gacattttct cttcgccctc acgaatgact acgtatttaa cttcaaccgc catttgcagc
    22621 ctctcataat aactgtgatt ttatacagta tatttctttt cggttgagaa atcaacatca
    22681 gcaataaaga cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa tcaggggata
    22741 ttcgttatga cgttattagg cactgcgctg cgtccggcag caactcgcgt gatgttatta
    22801 ggctccggtg aactgggtaa agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg
    22861 attgccgtcg atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc
    22921 attaatatgc ttgatggtga tgcattacgc cgtgtggttg aactggaaaa accacattat
    22981 atcgtgccgg agatcgaagc tattgccacc gatatgctga tccaacttga agaggaagga
    23041 ctgaatgttg tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt
    23101 cgcctggcgg cagaacagct gcagctgccc acttccactt atcgttttgc cgatagcgaa
    23161 agccttttcc gcgaggcggt tgccgccatt ggctatccct gcattgtaaa accggtgatg
    23221 agctcttccg gcaaggggca gacgtttatt cgctctgcag agcaacttgc tcaggcatgg
    23281 gaatacgctc agcaaggcgg tcgcgccgga gcgggccgcg taattgttga aggcgtcgtt
    23341 aagtttgact tcgaaattac cctgctaacc gtcagcgcgg tggatggcgt ccatttctgt
    23401 gcaccagtag gtcatcgcca ggaagatggc gactaccgtg aatcctggca accacagcaa
    23461 atgagcccgc ttgcccttga acgtgcgcag gagattgccc gtaaagtggt gctggcactg
    23521 ggcggttatg gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt
    23581 gaggtctccc ctcgtccaca tgataccggg atggtgacgt taatttctca agatctctca
    23641 gagtttgccc tgcatgtacg tgccttcctc ggacttccgg ttggcgggat ccgccagtat
    23701 ggtcctgcag cttctgccgt tatcctgcca caactgacca gtcagaatgt cacgtttgat
    23761 aatgtgcaga atgccgtagg cgcaggtttg cagattcgtt tatttggtaa gccggcaatt
    23821 aatggcagcc gtcgtctggg ggtggcactg gctactgcag agagtgttgt tgacgccatt
    23881 aaacgcgcga agcacgccgc cggacaggta aaagtacagg gttaaacccg gcaaaaaaac
    23941 gctacaaaaa tgcccgatcc gcgatcgggc attttgactt ttacagctta gcgccttcta
    24001 cagcttcacg cgccagctta gtaatacggt cgtaatcgcc cgcttccagc gcatctgccg
    24061 gaaccagcca ggaaccaccg atgcacagca cgcttttcag cgccaggtag tcacggtagt
    24121 tagccggaga aataccacct gtcgggcaga aacggacctg ggagaacgga cccgcgatcg
    24181 cctgcagggc tttcacgccg ccgttagctt cagccgggaa gaatttgaac tctttcaaac
    24241 cgtagtccat acccagcatc agttcggaaa cagtgctgat ccccggaatc agaggaatag
    24301 tcccttcggt agcagctttc agcagcggct cggtcagacc cgggctaatt gcgaactgtg
    24361 cacccgcttc ggtgacttct gccagctgct gtggattcag caccgtaccg gcacccacaa
    24421 tcgcttcagg cacttctttg gcgatagcac ggatagcgtc aactgcacac tcggtacgca
    24481 gagtcacttc cagaacgtgc accccaccag caaccaacgc ttttgccatc ggcactgcgt
    24541 gttccagttt ttttaccacg ataaccggta caaccgggcc ggtggtcagg attgattctg
    24601 cacttgtttt ccagtttttc atcagagttt tctctcgcct gattacaaat ttgtcgtctt
    24661 aaaaagtgat acaggttgcg ccctgttcgg caccggacag tttttcacgc aaggcgctga
    24721 ataattcacg tcctgtcccc acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag
    24781 cagccagttc cgcttcgtct accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa
    24841 tgatgtcccc gtcgcgcact tttgccagca gcccgccatc gtaggcttct ggtgttacgt
    24901 ggatagctga cggcactttt cctgaagcgc cggagagtcg tccatcggta actaacgcaa
    24961 ttttgaaaca ccggtccaat aatacaccaa gtggcggcat gagtttatgt aattctggca
    25021 ttccgttcgc ttttggcccc tgatgacgga caacaacgac acaatcgcgg tccagcaaac
    25081 ccgcttcaaa ggccggcata acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa
    25141 tcacctggtt ctcaaccggc acggcagagg ttttcataac cgcacggccc aggttaccgc
    25201 ttaacacttt tgtcccacca tgatgagaga aaggttgttc gaaggaagcg atcacattgc
    25261 tgtcgagtga tttttccgcc ccttcccgcc agtccagttc accattattc agccatggtt
    25321 caagggtata acgagacaga ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc
    25381 ctgctttgag cagttcacgc accagaaccg gtacgccacc tgccgcctgg aagtggttaa
    25441 tatcggccgg accgttcggg tagagacgtg ccatcagcgg tacaacatca gaaaggtcag
    25501 agaagtcatc ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg
    25561 tgtggttagt ggaaccaccg gtcgccagca gtgcaacgat accgttcacc accactttct
    25621 catcgatcat cttaccgatc ggcatccatt cattaccatt accggtcatg cgtgtaacct
    25681 gacgcgcagc ggcagcggtc aaagcatcgc gcagcggaga atccggatga acaaaagaag
    25741 agcctggcaa ctgcatcccc ataaattcca ccaccatctg gttggtgttg gcagtaccgt
    25801 agaaagtaca tgttcccggc gcatggtaag acgcggcttc tgactccagt aaggccatgc
    25861 ggtccacttt accttcggca taaagctggc gaatacgcac tttttcttta tttggcaaac
    25921 cgcttgccat cggtccagac ggcacaaaca ccgcaggcaa atgaccaaac gacagggctg
    25981 ccatcgtcag acccgggaca atcttgtcgc acacaccgag gaacagagca ccatcaaaca
    26041 tgttatggga cagcccgacc gccgcagaca tcgctatcac ttcgcggctt agcagcgaca
    26101 attccattcc atcctgcccc tgggtgacac catcacacat cgccggaaca ccgcccgcaa
    26161 cctgaccaac cgcattcgct tcatgcaggg ctttacgaat gatttctgga tagtgttcat
    26221 aaggctggtg cgcggagagc atgtcgttat aggaggtgat gatggcgata ttgttacgca
    26281 acatgctttt caaagaggct ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt
    26341 taccgcatgc caactgcgaa cgatgaacgg tcgaagtttt cgcttgttct atccgggcga
    26401 gataagcaga gcgagtctcg cgcgaacgtt caatgattcg atttgttacg cgtaacaatt
    26461 gtggattcat aaaggctcct gaaattgagt tgtcagagca ggatgattca caacgcgttt
    26521 cattcagagg atttatgact gaaacgcctg taaccggagc tcatagggca aaaacgtttc
    26581 agtcagtgta ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa
    26641 ttgttctaca atctgtgcaa gatcatgtta ccggtaaaat aaccataaag gataagcgca
    26701 gatattactc aaactcattc caggaacgac catcacgggt aatcatcgcc accgaggcaa
    26761 cgggtcccca ggttccggcc tgatacggtt tcggcgcatc attgtccatc gcccacgcct
    26821 cagtaatgga gtctacccat ttccaggctt cttccacttc gtcgcgacga acaaacagtg
    26881 cctgaatacc acgcatggtt tccagcagca aacgttcata ggcatccgcc agatgcgtct
    26941 gattaaaggt ttctgaatag ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt
    27001 caaggccagg aactttattc agtacctgga tatccacgcc ttcatcaggt tgcagacgga
    27061 tagtcagttt attctgcggc agatcctgcc acgattcttt aaacagattc agttcaggtg
    27121 ttttgaaata gaccacgact tcagaacatt tggtcggcag acgtttacca gtacgcaggt
    27181 agaatggcac accggcccag cgccagttat caatgtcgac gcggatcgct acgaaggttt
    27241 ctgtattgct gctcttgttc gcgccctctt cttccagata tcccggcact tttttgccct
    27301 gggcgaagcc cgcagtatat tgcccgcgta cggttttttc gcgtacgttg gagcggtcga
    27361 tgcggcgcag agacttcagt actttcactt tttcatcgcg gatgctgtct gcgctcaggt
    27421 cagacggcgg agacatcgca atcatgcaga gaatttgcag caggtggttt tggatcatat
    27481 cgcgcatctg accggcttta tcaaaatagc cccagcgccc ttcgatcccc acttcttctg
    27541 ccacggtaat ctcaacatga tcaatggtgc gattgtccca gttattcaca aacagggagt
    27601 tagcaaaacg cagcgccaac aggttcagca ccgtttcttt accaagatag tggtcgatac
    27661 ggtaaacctg gcactcctcg aagtattcgc caacctgatc attgatttcc tgcgaggtcg
    27721 ccagcgacgt ccccagcggt ttctccatga ctacgcgtgc cggtttagca ttcagtttag
    27781 cctcgccaag ccctttgcaa attgcgccaa aagtgctggg cggcatggca aagtagttaa
    27841 tggtgatacg atttttttga tccagcatca cgccgagacg gctgaatgca gcagtgtcat
    27901 tgacatcgag attacaaaaa tccagacgtg cactcagggt gtcccataaa ccttcatcaa
    27961 tggtttcttt catgaaagtt tcgagcgcct cgcggacaac tttggtatat gccgctttat
    28021 cccagtcagc acgccctacg ccgataatcc gggtgtccgg gttgagctga ccggcttttt
    28081 ccagttgata cagggaaggc agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa
    28141 tgaccaggtc acaggcctgg gctgtttgcg ttaccgccat gtcattctcc ttaagttaac
    28201 taacccggta cttaagccag ggtatacttg taattttctt acggtgcact gtactgcttt
    28261 tacgagcttg cgaaaactgt aaacgcttat ccacccgtgc gattacggta aaagcgcgca
    28321 aagtgcggca aaactgataa aaaaatcatc gttttccatt gggtaaaaat ctgacactga
    28381 tcatgttatg aaaaaaaata acaacttttt tatctgcttt tgtcattaac acagcacaca
    28441 ggcgtaatat ctgacaaaac tacatttcga tttctttcag tgcggaaatc gtcattaccc
    28501 gtgagtttct ttacatcatg aatatgctgg aaaaaatcca gtctcagctg gaacatttga
    28561 gcaaatcaga gcgcaaagtt gccgaggtca ttctggcttc gcccgataac gcgatccatt
    28621 cgagtattgc tgctctggcg ctggaagcca atgttagcga accgacggtg aatcgtttct
    28681 gtcgcagcat ggacacgcgc ggttttcctg attttaaact tcatctggca caaagtctgg
    28741 cgaatggcac tccctatgtt aatcgcaatg tcaatgaaga tgacagcgtt gaatcataca
    28801 cagggaaaat atttgagtcc gcaatggcaa cgcttgatca tgtccgtcat tcactggata
    28861 aatctgccat caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg
    28921 gattaggctc ttcagccgcc gttgcccacg atgcgatgaa taagttcttt cgttttaatg
    28981 ttccggtggt gtactccgat gatatcgtgc tgcaacgcat gagttgtatg aattgtagcg
    29041 acggagacgt ggtggtgctg atttctcaca ctggaagaac aaaaaatctg gtcgagctgg
    29101 cgcagctggc tcgcgaaaac gacgccatgg tgattgccct cacctctgcg ggtaccccgc
    29161 tcgcccggga agcaacgctg gcaattaccc tcgacgtacc ggaagatact gacatttata
    29221 tgcccatggt ttctcgactt gcacagctga ccgtgataga tgtgctggcg acaggattta
    29281 ctttgcgacg cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg
    29341 aatcgcgttt tgataagcag ttacttaatt taagtgacga tcgctaaaaa cgactgtcac
    29401 tgtcctaatc ttatacgaca tccgaatgag attaatttat cgccatcgcg gcgttatttc
    29461 attcggattt catgttcaag caacacttgg ttgtttcagt caacggagta ttacatgtcc
    29521 agaaggcttc gcagaacaaa aatcgttacc acgttaggcc cagcaacaga tcgcgataat
    29581 aatcttgaaa aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt ttctcacggc
    29641 tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg agattgccgc aaaactgggg
    29701 cgtcatgtgg ctattctggg tgacctccag gggcccaaaa tccgtgtatc cacctttaaa
    29761 gaaggcaaag ttttcctcaa tattggggat aaattcctgc tcgacgccaa cctgggtaaa
    29821 ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag gcctgcctgc tgacgttgtg
    29881 cctggcgaca tcctgctgct ggacgatggt cgcgttcagt taaaagtact ggaagttcag
    29941 ggcatgaaag tgttcaccga agtgaccgtc ggtggtcccc tctccaacaa taaaggtatc
    30001 aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt
    30061 aagactgcgg cgttgattgg cgtagattac ctggctgtct ccttcccacg ctgcggcgaa
    30121 gatctgaact atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa aattgttgcc
    30181 aaggttgaac gtgcggaagc cgtttgcagc caggatgcaa tggatgacat catcctcgcc
    30241 tctgacgtgg taatggttgc acgcggcgac ctcggtgtgg aaattggcga cccggaactg
    30301 gtcggcattc agaaagcgtt gatccgtcgt gcgcgtcagc ttaaccgcgc ggtaatcacg
    30361 gcgacccaga tgatggagtc aatgattact aacccgatgc cgacgcgtgc agaagtcatg
    30421 gacgtagcaa acgccgttct ggatggtact gacgctgtga tgctgtctgc agaaactgcc
    30481 gctgggcagt atccgtcaga aactgttgca gccatggcgc gcgtttgcct gggtgcggaa
    30541 aaaatcccga gcatcaacgt ttctaaacac cgtctggacg ttcagttcga caatgtggaa
    30601 gaagctattg ccatgtcagc aatgtacgca gctaaccacc tgaaaggcgt tacggcgatt
    30661 attaccatga ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag ctctggtcta
    30721 ccaattttcg ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct ctatcgtggc
    30781 gttacgccgg tgcactttga tagcgctaat gacggcgtag cagctgccag cgaagcggtt
    30841 aatctgctgc gcgataaagg ttacttgatg tctggtgacc tggtgattgt cacccagggc
    30901 gacgtgatga gtaccgtggg ttctactaat accacgcgta ttttaacggt agagtaatct
    30961 cacctgcccc ggccgctgat gaccggggta tttagcaaca cataaattag gactactcat
    31021 tcaatccaat cataatcaca gccccctgta aaatgaatac agggatagcc tgaatggaga
    31081 tctaaatagc ttgcaagacg tcgcataccc gcttcctctg caacactata tccaataact
    31141 gccatcggta agccactatc tttggagtat tgtgtctgca cccatgtttt aattgaatcg
    31201 ttaatcgtaa tataataatc agcctgatat tccatatatt gtattggatc acaaaaacag
    31261 cctgccccaa tcccaatagt tctaactatt ctggctctat ctccataaaa ttggatccca
    31321 ggaagattat aaattttcaa atttttggca aaacgtttgg taatattaat ggcactgtca
    31381 ggttctatag catatacatg gtacatatca tcagaagcaa caatatttgt aacatccagt
    31441 tctaactgct gagccaaagc cttagacatt ccgaacgtag gctctcgatc catgacatca
    31501 tggcaacgaa tgattaccaa attattttta tgcaaaaact cctttttttg ctcaattatt
    31561 tttaaataac tgagttcacc gcgcgtataa tccattattt ttctggccca tgaaaactca
    31621 agaacgtcac cctccagatc agcataagaa taaaatgtag gctcatgagt aataatgata
    31681 ttggcacctt caaaaacagc ctttcttaaa acatcaattg aggcaatcca gcaggtccca
    31741 attttattaa taagtgtcgc ctcagcacca aaaataattt tatcaacagt tggttgcgaa
    31801 acggcaacca ttgaatttaa aaaacaatta agatctgcgg ctgttttaat atgatgcatt
    31861 caacatcctt caccggtttc atcagcaata aaataatttc aacatttctg gaaaacgtta
    31921 tgcccatgca gactcagagt gggtatcacc tccccataga tgataatcta tagttacatt
    31981 gcgttgttta cggagactca cgcataattt ttccgcgcct gaaatataga cttcaggaaa
    32041 gtcttcgcgc ataccactgg tttccagcgt accgacatcg agatatacag taagatctgc
    32101 aaagatattg ttttcctgaa taaaattcag cattgcgctg tcattgaacc atagtgccgg
    32161 cgaaaaaacg ccaacttttg aaaaaacgtc tagacgaaga attgcggcaa acaaactgat
    32221 gtatgcaccc atagaactac ctgctatcgc tgtattatta cgatctggga gagttcgaaa
    32281 attgctgtca atatagttct tgagcgtatt cacaataaaa tcaacataca acgcaccttt
    32341 tcctccacta taatccacat cagggtccca gccaggtaag ggaaaagatg tgtttcgcgg
    32401 ccatggagaa tactcatcca tacggctgaa tttaccatga gacgatgcat tatcaatccc
    32461 cacaatgata acaccgtcgt attgggaatt tgggaaaaaa gcatctaccg cctcaggtat
    32521 atcccataca tgtggaccaa aagatacaga tttatcaaat aaattttgcc catcgtgcat
    32581 atacaataca gggtaactga catcagattc ataataggac gcaggtagta atattcttat
    32641 ttcgcgacta cttgccaatt gtggcatcgg aaaggattgc aacacatgat atcgattccc
    32701 atagttctca atatcgttca taatttaaat taacctcaat aaacaaaaga aatattacat
    32761 ctaagcactc ccctaccaca ttatatatgg gagcacttag aaatgactaa tatctatatt
    32821 ttta
//
LOCUS       DAZUFB010000047        32390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZUFB010000047 DAZUFB010000000
VERSION     DAZUFB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.047"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(188..316)
                     /gene="rhuM"
                     /locus_tag="ACHY3M_002481"
                     /pseudo
     CDS             complement(188..316)
                     /gene="rhuM"
                     /locus_tag="ACHY3M_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(395..489)
                     /locus_tag="ACHY3M_002482"
     tRNA            complement(395..489)
                     /locus_tag="ACHY3M_002482"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(453..455),aa:Sec,seq:tca)
     gene            782..2164
                     /gene="yicJ"
                     /locus_tag="ACHY3M_002483"
     CDS             782..2164
                     /gene="yicJ"
                     /locus_tag="ACHY3M_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFS4317385.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2174..4492
                     /gene="yicI"
                     /locus_tag="ACHY3M_002484"
     CDS             2174..4492
                     /gene="yicI"
                     /locus_tag="ACHY3M_002484"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFS4317386.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4545..6254)
                     /gene="yicH"
                     /locus_tag="ACHY3M_002485"
     CDS             complement(4545..6254)
                     /gene="yicH"
                     /locus_tag="ACHY3M_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS4317387.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6375..7766)
                     /gene="xanP"
                     /locus_tag="ACHY3M_002486"
     CDS             complement(6375..7766)
                     /gene="xanP"
                     /locus_tag="ACHY3M_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFS4317388.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8046..9251
                     /gene="gltS"
                     /locus_tag="ACHY3M_002487"
     CDS             8046..9251
                     /gene="gltS"
                     /locus_tag="ACHY3M_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFS4317389.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9254..10120
                     /locus_tag="ACHY3M_002488"
     CDS             9254..10120
                     /locus_tag="ACHY3M_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317390.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10105..12186)
                     /gene="recG"
                     /locus_tag="ACHY3M_002489"
     CDS             complement(10105..12186)
                     /gene="recG"
                     /locus_tag="ACHY3M_002489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4317391.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12192..12881)
                     /gene="trmH"
                     /locus_tag="ACHY3M_002490"
     CDS             complement(12192..12881)
                     /gene="trmH"
                     /locus_tag="ACHY3M_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFS4317392.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12888..14996)
                     /gene="spoT"
                     /locus_tag="ACHY3M_002491"
     CDS             complement(12888..14996)
                     /gene="spoT"
                     /locus_tag="ACHY3M_002491"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4317393.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15015..15290)
                     /gene="rpoZ"
                     /locus_tag="ACHY3M_002492"
     CDS             complement(15015..15290)
                     /gene="rpoZ"
                     /locus_tag="ACHY3M_002492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4317394.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15345..15968)
                     /gene="gmk"
                     /locus_tag="ACHY3M_002493"
     CDS             complement(15345..15968)
                     /gene="gmk"
                     /locus_tag="ACHY3M_002493"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4317395.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16226..17908
                     /gene="ligB"
                     /locus_tag="ACHY3M_002494"
     CDS             16226..17908
                     /gene="ligB"
                     /locus_tag="ACHY3M_002494"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFS4317396.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17905..18522)
                     /gene="yicG"
                     /locus_tag="ACHY3M_002495"
     CDS             complement(17905..18522)
                     /gene="yicG"
                     /locus_tag="ACHY3M_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFS4317397.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18814..19638)
                     /gene="dinD"
                     /locus_tag="ACHY3M_002496"
     CDS             complement(18814..19638)
                     /gene="dinD"
                     /locus_tag="ACHY3M_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFS4317398.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19859..20722)
                     /gene="yicC"
                     /locus_tag="ACHY3M_002497"
     CDS             complement(19859..20722)
                     /gene="yicC"
                     /locus_tag="ACHY3M_002497"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4317399.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20849..21565
                     /gene="rph"
                     /locus_tag="ACHY3M_002498"
     CDS             20849..21565
                     /gene="rph"
                     /locus_tag="ACHY3M_002498"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4317400.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21631..22272
                     /gene="pyrE"
                     /locus_tag="ACHY3M_002499"
     CDS             21631..22272
                     /gene="pyrE"
                     /locus_tag="ACHY3M_002499"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4317401.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22309..22905)
                     /gene="slmA"
                     /locus_tag="ACHY3M_002500"
     CDS             complement(22309..22905)
                     /gene="slmA"
                     /locus_tag="ACHY3M_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFS4317402.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23012..23470)
                     /gene="dut"
                     /locus_tag="ACHY3M_002501"
     CDS             complement(23012..23470)
                     /gene="dut"
                     /locus_tag="ACHY3M_002501"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4317403.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23448..24668)
                     /gene="coaBC"
                     /locus_tag="ACHY3M_002502"
     CDS             complement(23448..24668)
                     /gene="coaBC"
                     /locus_tag="ACHY3M_002502"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFS4317404.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKLKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIMTRYDEKNRR"
     gene            24840..25508
                     /gene="radC"
                     /locus_tag="ACHY3M_002503"
     CDS             24840..25508
                     /gene="radC"
                     /locus_tag="ACHY3M_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4317405.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPAMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25725..25961
                     /gene="rpmB"
                     /locus_tag="ACHY3M_002504"
     CDS             25725..25961
                     /gene="rpmB"
                     /locus_tag="ACHY3M_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4317406.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25982..26149
                     /gene="rpmG"
                     /locus_tag="ACHY3M_002505"
     CDS             25982..26149
                     /gene="rpmG"
                     /locus_tag="ACHY3M_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4317407.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26247..27056
                     /gene="mutM"
                     /locus_tag="ACHY3M_002506"
     CDS             26247..27056
                     /gene="mutM"
                     /locus_tag="ACHY3M_002506"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFS4317408.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27095..27574)
                     /gene="coaD"
                     /locus_tag="ACHY3M_002507"
     CDS             complement(27095..27574)
                     /gene="coaD"
                     /locus_tag="ACHY3M_002507"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4317409.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27582..28859)
                     /gene="waaA"
                     /locus_tag="ACHY3M_002508"
     CDS             complement(27582..28859)
                     /gene="waaA"
                     /locus_tag="ACHY3M_002508"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFS4317410.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29272..30330
                     /gene="rfaQ"
                     /locus_tag="ACHY3M_002509"
     CDS             29272..30330
                     /gene="rfaQ"
                     /locus_tag="ACHY3M_002509"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFS4317411.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30327..31451
                     /gene="waaG"
                     /locus_tag="ACHY3M_002510"
     CDS             30327..31451
                     /gene="waaG"
                     /locus_tag="ACHY3M_002510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4317412.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            31444..32241
                     /gene="rfaP"
                     /locus_tag="ACHY3M_002511"
     CDS             31444..32241
                     /gene="rfaP"
                     /locus_tag="ACHY3M_002511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFS4317413.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7664 a         8035 c         8687 g         8004 t
ORIGIN      
        1 ttgatatatt aaatgaacaa attaatcttg atggcagtct gattattggt gaagtttata
       61 gtcagttttt ttcgccagcg gttaaagaaa tagaatagtc gtataaaaaa gggtgttctg
      121 gggcgagtaa aacaacatct tcaccttaat ggcgatacag ttggctgaga gccagcaggg
      181 ggcaaatcac ctgcaaataa acacggcaca tgctggcgcg catatcgcgg atgcgttcga
      241 ggacagaaga accgacaggc ggattcttca gccgctcatc gtccatcaca aaaccttaga
      301 ccaggtattc ctgaagccga ttgaatattg agcagtagaa aatatctgga ttgacagtat
      361 tacaaggggt tggagaacgt cagaagaggc attttggcgg aagatcacag gagtcgaacc
      421 tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca ccggagacga
      481 cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta cttccttgag
      541 tttctacatc ccccagatca gttttttccg cctaatacac gcagcgtatt atcacgaaag
      601 cattatagtt catataaaac ctatatttta caatgatata tgagcgaaat taacgctacc
      661 acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat tcgctctcac
      721 agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca aggaaaagca
      781 gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg gagacgccgc
      841 cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata ccgatatttt
      901 tggcattcct gccggttttg tcggaaccat gtttttggtc gctcgtgcac tggatgcgat
      961 ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg
     1021 tccgtgggta ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg cctatagcac
     1081 gccagatctc agtatgaacg gcaaaatgat ctatgcagca attacttaca ccctacttac
     1141 cttactttat accgtcgtca atatccctta ctgcgcattg ggtggtgtaa tcaccaatga
     1201 cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat
     1261 gctttctact gttctgatga tgccactggt taatttaatt ggcggtgata ataaaccact
     1321 cggtttccag ggcggtatcg cggtcctttc cgtggtggca ttcatgatgc tggcattctg
     1381 tttcttcacc actaaagaac gcgttgaagc accacctaca acaacgtcta tgcgggaaga
     1441 tttacgtgat atctggcaaa acgaccagtg gcggattgtc ggtttactaa ccattttcaa
     1501 tatcctggcg gtgtgcgtac gcggtggggc gatgatgtat tacgtcacat ggattttggg
     1561 cacgccggaa gtgtttgtcg cttttctcac cacttattgc gtgggtaacc tgattggttc
     1621 cgcactggca aaacctctga ccgactggaa atgtaaagtc actatcttct ggtggacgaa
     1681 cgccctgctg gcagtgatta gcctcgcgat gttctttgtt cccatgcagg ccagcatcac
     1741 tatgtttgtc ttcatcttcg tgattggtgt gttgcatcaa ctggtgacac ctatccagtg
     1801 ggtaatgatg tccgataccg tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg
     1861 gatcagtttt gctggcacgc tgtttgtgct caaactgggg ttggccttcg gcggcgctct
     1921 tatcggctgg atgctggctt atggcggata tgatgcggca gaaaaagcgc agaacagcgc
     1981 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
     2041 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
     2101 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
     2161 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
     2221 gattcacccg cttcaggtat tcgaggttga acagcagggt aatgaaatgg tggtctatgc
     2281 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
     2341 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
     2401 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
     2461 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
     2521 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
     2581 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
     2641 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
     2701 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
     2761 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
     2821 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
     2881 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
     2941 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     3001 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     3061 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     3121 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     3181 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     3241 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     3301 gtgggataaa tggcagccag gtctggcgat ttatgacttt accaatccgg atgcctgcaa
     3361 atggtacgcc gacaaactga aaggtctggt cgcgatgggc gttgattgct ttaagaccga
     3421 ctttggcgaa cgtatcccaa ctgatgttca gtggtttgac ggttccgatc cgcagaaaat
     3481 gcataaccat tatgcataca tctacaacga actggtgtgg aacgtgctca aggacaccgt
     3541 tggtgaggaa gaagctgtct tgtttgcccg ctcggcctcc gtcggtgcgc agaaattccc
     3601 ggtacactgg ggtggcgatt gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg
     3661 tggtttgtct attggccttt caggttttgg cttctggagc cacgatatcg gcggctttga
     3721 aaataccgct ccggcgcacg tttacaaacg ctggtgcgcg tttggtttgc tctccagcca
     3781 tagccgttta cacggtagca aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg
     3841 tgatgtagtg cgcttcttca cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga
     3901 agctgcgcgt gcgaacgcgc ggggtacgcc gatgatgcgg gccatgatga tggagttccc
     3961 ggacgatccg gcttgtgatt accttgaccg tcaatacatg ttaggcgaca acgtgatggt
     4021 tgcgccggtg ttcactgaag cgggcgatgt gcagttctac ctgccggaag gtcgctggac
     4081 acacctgtgg cacaacgatg aactcgacgg tagtcgctgg cataaacagc agcacggctt
     4141 cctgagtctg cccgtttatg tgcgtgataa cactctactg gcgctgggca acaacgatca
     4201 acgtcccgat tacgtgtggc acgaaggcac ggcattccac ctctttaatc tgcaagacgg
     4261 gcatgaagcc gtctgtgaag tgcccgctgc tgacggatcg gtgatcttta ctttaaaagc
     4321 agcacgtact ggcaacacga ttactgtgac tggtgcgggc gaggcgaaga actggacact
     4381 gtgcctgcgc aatgttgtga aagtaaatgg tctgcaagac ggttcgcagg ctgaaagtga
     4441 gcaggggctg gtggtgaagc ctcaagggaa tgcgctgaca attacgttgt aatctattga
     4501 tggaatgccg ggtacgtctg agtcgtaccc ggcgagattg atgtttactg caccagctct
     4561 gccgacgtaa cttcaccgct ggaaacgatg ccagcattca tggtttgagt cacttgctgc
     4621 tttgcggcgt tcacggcatg caattgcccg ctaaccgtag gtttcagcgg ggcattggcc
     4681 tgaatatctc cactggcggt aagctgaata ttgccgtcgc cagtcaacgg taattcaggc
     4741 catccccatt gttgcaaaat attcaccggc acaccgcgtc cattcaggct gatatgggtc
     4801 tgacgttgtg gcgtttgtga aacactggca gtggcttcca gaatgccttt ttcagtaaat
     4861 gcactcagtt cgctgatatt caccgtgctg ctgttggcgg tcagcgccag cgacgggcga
     4921 cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca gattcgccga gccactccag
     4981 acgccccatt tatgatcggt aaccagcgtc aggttggcac cgtaaccatc gagcgcggtg
     5041 agctgccacg ggaagtcagg gtcgatatca atgatcagat tgcggctggc gctaaatctc
     5101 ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca tccacaactg ttgccagttt
     5161 ttcggcaagg tatattccag cccggcaatt gccacatcat caaggatcaa cgttttcccg
     5221 tcacgcagcc agttccctga cgttctgacc ataccccctt cccagcggct ggtgaactgg
     5281 cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa tcgggtcaaa taaatgcagc
     5341 gaaccataaa tgaactcgct agcgttcatc gacagtttgc cttcctgtgt ctgccagtca
     5401 tctttactga aggtcatgtt gcgcaagctg agatcgaggt cggtcaccgc ccagtccgga
     5461 ccttgcaaac gagcatcgat cacttccagg cgaccaatct gcaacgacgg gacagagcgt
     5521 aatggcgcaa agaagtcggt tagcgatttt tcgctttgta gacgtatatc cgccatgcgc
     5581 aggttttcca cttgccagct gccgtcggcg ttacgctgcg cgtttccggt taatgtcccg
     5641 cgggcgatgt cggcacccag gttagtcagc gtaacgcgat cgttatcaat actgccttca
     5701 atcagtacat tggtggcagg aacatcattg agcgaaagcg atccggcact aaactgaatc
     5761 tgcgccttcg tacccagcac tttaccggct tttggtgacc acggaactac gccgccattt
     5821 acccgctgcg cgctcagttt ccattcgcta ttcgggctat taaacgccat atcacgcagt
     5881 tgcagccgat cggctttgaa cggtagcggc gcggtctggt cggtgagatt cagcgtcccg
     5941 ttttccagca ggatggtatc gacatggcgt ggttcggtca gttgccgact gcttagcgca
     6001 atgtcgacac tttttgccac cagggtcgcg ggctggccat cacggccaaa cgtgacgttc
     6061 tccagcacga tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa ggccagatga
     6121 tagtcgctat tctcggaaac ccatgcgctg atatgttctg ctccccagcg ggtttgcaga
     6181 agaaaataga ggccagcgat caccactaac agagcgatga gaatgtagag aagcagcttc
     6241 ccaataaatt tcatggtctt ccatcccgtg aaatgcacat aagggagtta tgcacgattt
     6301 acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga ctgcggcggt aattatctta
     6361 ccgccgccag tgaattactg tttttctggt gggaaaatca gattcagcac gatggcagta
     6421 ataccgcccg cggcgatccc ggaggagagc aggtttttca gccattcagg ggcaaactgc
     6481 aaaatcagcg gctgctgaga cacgcccaga ccaaccgcca gcgacagcgc gataatcaga
     6541 atcgcccgac ggttcagcgg ttcacgagaa acgatacgca caccggaggc ggcgatagtg
     6601 ccaaacatta caagcgttgc gccgcccaga accggttctg gaatgtgttg tacaaaaccg
     6661 ctcactgccg ggaacagacc cagcacgatc aacatcagcg cgacgacaaa accgacatag
     6721 cggctggcaa caccagtcaa ctggatcact ccgttgtttt gcccgaagca ggagttcggg
     6781 aaggtgttaa acaccgccga aacaaacgag ttcaggccgt ttgccagcac gccgcctttc
     6841 aggcgtttca tgtacagcgg accggacact ggctgttcgg aaacgtcaga ggtcgccgtg
     6901 atatcgccaa tggtttccag cgaagtgatc ataaagacca gcatcagcgg cagcagcaga
     6961 ctccattcaa tgccaagacc gtaatagagc ggcgttggca ccataatcag ttcttgcgtc
     7021 atcggttcgt tgctttctgg caacatgccc ataaaccacg ccagcgcata tccggccgcc
     7081 atcgcaatca ccagtgaggc cacgcgtaag taagggttac gttgacggtt aagcaggata
     7141 attaaggcta agaccacgcc tgccagcagc agatttttcg gtgcgccgaa ggtgttatcg
     7201 ctcatggctg cgtaaccgcc gccaatggac gttaacccaa cctgaattag cgacaggccg
     7261 ataatcatca ccacaacgcc agaaaccagc ggcgtaataa tgcggcgcgc cagatgcaga
     7321 acgcgggaga tcaccatctc ggtgcaactt gccagcatca acgtgccgaa caaagccgcc
     7381 atcatggtag gaacatcagc accaccggtt ttcagcgcgg taccgcccat aatcagcggg
     7441 gcaacaaagt tgaagctggt gccctgaata gacaacagcc cggagccaac cggaccccag
     7501 gccttaattt gaataatcga tgccacaccg gaggcaaaca gcgacatact aataatgtgt
     7561 tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta atagcgctgg cgtgatcacc
     7621 gcaacgaaca tcgccagcag atgctgacag gcggcaaaca gggtttgagg aagcggcgga
     7681 cgatcttcaa gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg ttgcgcattt
     7741 tctgactcga gggtggaaac agacatcgaa aacaatcccg tggtagcaaa gcgggcattt
     7801 tagctgaccg aaagagaaaa gcaaacgttt gccacatcct catctaaata tttaaatgag
     7861 ataaatgcac ttttttatat aacttttggt atttttctgc ttaaaatcca tgccatttgt
     7921 tcattaacag ctgagaattt gacccaggaa agcataactc gatacccgtc atagttcatg
     7981 aagttgccac tgcgcgatga agtatgacga gtatgaaaga gtgatgcgga tacaaaggag
     8041 taactatgtt tcatctcgat actttagcaa cgcttgttgc cgcaacgctg acgttgctgc
     8101 tcgggcgtaa gttggtccat tccgtctcct ttttgaagaa atacaccata ccggaacctg
     8161 ttgcgggtgg tttgttggtg gcgctggcgc tactagtact gaaaaaaagc atgggctggg
     8221 aagtcaactt tgatatgtcc ctgcgcgatc cgttaatgct ggctttcttc gccaccattg
     8281 gcctgaacgc caacattgcc agtttgcgtg ccggtgggcg tgtggttggc atcttcttga
     8341 ttgtggttgt tggtctgttg gtgatgcaaa atgccattgg cattggtatg gctagcctgt
     8401 tagggcttga tccgctgatg gggctgttgg ccggttctat tacgctttcc ggcggtcacg
     8461 gtacgggcgc tgcgtggagt aaattgttca ttgaacgtta tggcttcacc aatgcgacgg
     8521 aagtggcgat ggcctgtgca acgttcggtc tggtgctggg cggcttgatt ggcggtccgg
     8581 tggcgcgcta tctggtgaaa cactccacca cgccgaacgg tattccggat gaccaggaag
     8641 tcccgacggc gtttgaaaag ccggatgtgg gacgcatgat cacctcgctg gtgctgattg
     8701 aaactatcgc gctgattgct atctgcctga cggtggggaa aattgttgcg caacttttgg
     8761 ctggcactgc ttttgaactg ccgaccttcg tctgtgtact gtttgttggc gtgattctga
     8821 gcaacggtct gtcaatgatg ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg
     8881 gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat ggggctgaaa ctgtgggagc
     8941 tggcttcgct ggcgctgccg atgctggcga ttctggtggt acagaccatc ttcatggcgt
     9001 tgtatgccat cttcgttacc tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg
     9061 ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac ggcaatcgcc aacatgcagg
     9121 cgatcactga acgctttggc ccgtcgcaca tggcgttttt ggtggtgccg atggtcggtg
     9181 cgttctttat cgatatcgtc aatgcgctgg tgattaagct gtatttaatg ttgccgattt
     9241 ttgccggtta aaaatgaaaa agcgtcggaa ggaaccggaa acgctacgtg aacactgtcg
     9301 ccatattttt ggtgatgagc cgccagtgct ctgtgtatgg gaaacggaat ttgattacgc
     9361 agatgccgaa ctcaaggcgc tggcggctaa agagtggcag cagatcagcg aatgggattt
     9421 gagtgcttac tatgtactca atctcgttta taacgaaccg atgcaaatag agttgtttcg
     9481 ctatctgttt ccgctctgcc tggcgcaatg gcatgaaacc gttttagctg gcggttatgg
     9541 tgatcacttc gaagaatcct taatgaaggc attgtgtcgg ccctatctct ggcaggagat
     9601 gatgaatgct tcccaacgcc agcaggtacg gcaattttta ctggacaccg cgttgcagcg
     9661 tatggataac gagcgtggtt tcaataacgt tctttgctgg ctggcggtgt tcaatactct
     9721 gggtggggcc gcgccgttaa ttcgttctct ctggtcgcgc tggtgggcgc tggatacgcc
     9781 aggaaaagcg gtgtgtgcta ttcagtatgc cgcgcatcta atttatccca tagaagccaa
     9841 tccgttatgg tcgcaagaat ggataggctg ggggcatcca ttaggccata aagatggctg
     9901 gtcctctgat aatcgcgctt ttcttcgcca aatgttaaca cctgaaatga ttgtggctgg
     9961 cgtacaggca gcggctgaaa ttctgcgtgg tgaaccggaa ggcgcaatgg cggcgcgaat
    10021 tgcacaggat gcgtatgagg caatggatat cctcactatc cagattgaag atttactgcg
    10081 tgacttgtct tgtgatgagt ctggtcatgc gttggagtaa cgttccgtct ccggcatcca
    10141 gcgttctatc agggcttttg cttgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg
    10201 ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc gccactttaa actcagcatt
    10261 acccgtctga cgcgtaccta acagttcacc cggtccgcga atctctaagt ctttctgtgc
    10321 aatcacaaaa ccgtcgttgc tgtcgcgcag cacttgcagc cgaatttgcg cggttttgga
    10381 gagcggcgtt ttgtagagca gcacgcagtg agaagccacc gcaccacgac ctacacgccc
    10441 gcgaagctgg tgtaactgcg ccagacccag acgctccggg ttttcgataa tcatcagact
    10501 ggcgttaggc acatcaacgc cgacttcaat aacggttgtg gcaaccagca ggtgtagctc
    10561 accttgttta aacgacgcca tcaccgcctg tttctcggca ggtttcatcc gcccgtgtac
    10621 caggccaacg ttcaactctg gtagcgccag tttcaactct tcccaggtag cttccgccgc
    10681 ctgcgcttcc agcaattccg actcttcaat caacgtacaa acccagtatg cctgacgacc
    10741 ttcagttatg caggcgtggt gcacgcggtc aatgatgtcg gtacggcggg tatcaggaat
    10801 agcgaccgta gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag
    10861 atcggcatac gcagtcattg ccagcgtgcg ggggatcggc gtggcggtca tgatcaactg
    10921 atgcggatgg aagccctgct gctggccttt ctcccacaat gccagacgct gatgcacgcc
    10981 aaaacgatgc tgttcgtcga taatcaccag cgccaggccg ttaaactgca cctgttcctg
    11041 gaagatggcg tgtgtaccga caatcatctg cacctgaccg ctggcgatgg cttcctgctg
    11101 tgccagccgt gctttacctt tctgcttacc ggcgagccag cccacttcga taccgagcgg
    11161 tgcaaaccag ttgcggaagt tattggcgtg ctgctcggcg agtaattcgg ttggtgccat
    11221 caatgctacc tgtttgccgt gggcaatcgc acgcaacgcg gcgagggcgg cgaccagcgt
    11281 tttaccggaa cctacatcgc cctgcaccag acgcatcatc ggcacatcca gcgccatatc
    11341 gcgctcgatc tccgccacta cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggcggc
    11401 gaggagttta tttttcagcg tgtcattggc gctcagcggc tgggcatgaa aacgctgtgc
    11461 tccggcacgt aaggctaaca tgctgaggtt gtgcgccagc agttcttcca gaatcagacg
    11521 acgttgcgcc ggatgctgcc cggtttccag atcgctaagc tgtagcgtcg gtggcgggcg
    11581 gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag
    11641 gagttcttca atggcacagg tgtcgagcag atccagcgcc tggtcggtta atttacgcag
    11701 cgtggcctgc tttacgcctt ccgttgttgg ataaaccggc gtgagcgttt cctgtaattc
    11761 tggcgtgctg agatcgccct gcacgcggta ttccgggtgg atcatctccg caccatattt
    11821 accgcgcttt gcttcgccat aagccagtac acggcggccc gccgccaggc tatttttcat
    11881 tgccgcgctg aaattgaaaa agcgcatggt gaggatgccg gaaccgtcgc tgatctggca
    11941 ggtcatcatc cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt
    12001 ggcataaacg cccggcagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg
    12061 caggggaagg tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagtttgtt
    12121 actaagtgct gcgccaacgc ccgttaggga actgagtggg acagcatcta acaggcgacc
    12181 tttcatggca cttaccctgc agcctgcata gtagcccacc agtcggcatc agcttcgatc
    12241 tcgccttgct gattgacgtg gggataaggc aggcctttgc gttttgcgac tttcgccagc
    12301 accggatagc cgccttcaaa caacaggcgt tgttgctctg cttccggcaa catgctgttt
    12361 tcacgcaggt acatgcctgc attttgccgc tgacgctggg cttcgtaaag aatgagggct
    12421 gaggcaacgg acacattcag cgactgcacc atgccgatca tcggaatgat gatgtcctga
    12481 tccgccaggg ccaatgcttc ctgcgtgatg cccgttttct cctgtcccat caaaatgcag
    12541 gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt tatcagaaag atgggttgcc
    12601 agaatctgca tgccctggcc tttgagatga gcgacggcat cgccaatggt gcggtgtgtt
    12661 ttcacctgta cccagctgtt actacccgcc gctgccgaag ccatggtgcg catgcggcta
    12721 ccaggccaga cggcgtgaac ttcatgtacg ccaacggcat ctgcggtacg aataatcgca
    12781 gaaacgttat gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg
    12841 gcgagcattt cgcagatgcg tgcataacgt gttgggttca taaaacatta atttcggttt
    12901 cgggtgactt taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc
    12961 acacggtcac gagcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc
    13021 gtattcaaac tttgaatatt cgaggtcgtg gtgttaattg ccgccgtcag gtttgccagc
    13081 gcaccctgat gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct
    13141 ttatcccatt ccacagccat aaacttctct ggctctttct ggtagccacg gatgttacgg
    13201 caggattcat ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg
    13261 ccaggaatgg ggcggcagca tttcgcaaag gtgatcagca cgccatcagc gcctttgatc
    13321 ggcagatgcc cgtggctttg agtcgccggt ggaatggagg cgtccccatg ttgtagattt
    13381 ttcgcgacca ccacgctcat tgcgttacca aggccgattt ctgccagcag atcgtcaagc
    13441 gttgccagct tcatgcgatc cagctcgcgc tgaatatttt cctgcgggat ttcattgagc
    13501 ttacggctac cacccaaagc atggttgagc agacgacggc ccaggcttac agaatcatca
    13561 cgcttgaggt ttttcagcaa ctgacgaatt ttggcgcgcg ctttcgagct aacgacaaag
    13621 ttcagccaag cggcattcgg gcgagcgccc ggagcggtaa tgatttcaac ggtctgaccg
    13681 ctagtaagcg gctgcgacag cgggtaaggc tggcggtcga cgcgtgcgcc cacgcaggca
    13741 tggccgatat cggtatgcac tgcataagcg aagtcgacgg gcgttgcacc ggcaggcagc
    13801 tcgacaatgc gcccttccgg tgtgaaaacg taaatctcat ccgggaagag atcggattta
    13861 acgctctcga taaattcaaa cgaactaccg gcgctctgtt gcagctccag caggctttgc
    13921 atccagcgct gggcgcggat ttgcgcggtg gtgctggttt cgccgtgctc tttataagcc
    13981 cagtgcgcgg caacacccat ctccgccatc tgatccatat cttcggtacg gatctggacc
    14041 tcaaccggca caccgtgcgg gccgatcatc gaggtgtgca aagactgata gccgttcgct
    14101 tttggaatgg cgatgtagtc tttcacgcga cccggacgcg gcttgtacag gctatgcatc
    14161 tgcccgagca cgcgataaca ggtgtcggaa tcattgacga tcacgcggaa agcgtagatg
    14221 tccatgattg agtgaaaacg ctgctctttg agcaccattt tgcagtaaat cgaataaaga
    14281 tgcttctcgc gaccactgac gcggcacgga attcccgctt cctgcaaacg cccttcgatt
    14341 tcagagagga ttttttggat catctcttta cggttgccgc gcgcggcttt caccacttct
    14401 ttaattacgc ggtaacgatt gggatacagc gcctcaaaac ccagctcttc gagttcggtt
    14461 ttaatgtggt ggatacctaa acggtgcgcc agcgggctgt aaatttcgag agtttcacgg
    14521 gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg
    14581 tcggcaagtt tgatgaggat gacgcggata tcctgcacca ttgccataat catcttgcga
    14641 aagttttcgg cctgcgcctc tttcttatca cggaacttga gtttatcaag tttcgacacc
    14701 ccctctacca gctcggcgac gcttttacca aaaagctgtt ccatatcctg gtaggtggcg
    14761 ggagtatctt caatcacgtc atgcagcagc gccgccatca gcgtttcata gtcgagtttc
    14821 atctcggcca gaatgcaggc aaccgctacc gggtgcgtga tatagggttc accgcttgaa
    14881 cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt
    14941 tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc
    15001 gacccgcttt gtgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg
    15061 gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga
    15121 ccttcttcga tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc
    15181 ggatcctttc cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca
    15241 aaacggttac caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag
    15301 ctccacaggt gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat
    15361 ttgctgatta aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga
    15421 ataatggtct tcaaatcggt caacgcggta tcgaagtcat cattcacaat cagataatca
    15481 tattcggcgt aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct
    15541 tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt caattttgga cggcggtaaa
    15601 ataaagatac tccgcgcgtg cggcatcttc tggcgaattt gctgcgcgcc ctgccagtcg
    15661 atatcgagaa aaacatcgac accggtcgcc agtacttgct caatggcctc acgcgaagtg
    15721 ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct
    15781 ttaaattcat catgattaac aaagaaataa tgttcaccgt ggacttcacc aggacgcggt
    15841 tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa
    15901 gcctgaatca ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct
    15961 tgagccatga gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta
    16021 tacacggcgc ggccatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt
    16081 tttgcgtagc tgtttccgca tttgcctgtg atttactgca ataaagacga ttctcaggaa
    16141 gcgtcctcgc agactccgtt tttgccactc ggctttgcct tcaccacgtt gtatatctgc
    16201 cagcctgact acagcggagg tgaagatgaa agtatggatg gcgatattaa taagtatctt
    16261 gtgctggcaa tcatctgtgt gggcggtctg tccggcctgg tcgccagcca gagcacagga
    16321 agaaatttcc cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaggaagg
    16381 aaaaagtgag gtggaagacg gtgtttacga tcagttaagc gcccgtctta cgcagtggca
    16441 acgctgcttt gtgagcgagc cccgcgacgt gatgatgccg cctttaaatg gtgcggttat
    16501 gcatccggtt gcccatacgg gtgtacgcaa aatggcggat aaaaacgcat taagtctgtg
    16561 gatgcgagaa cgtagcgatc tttgggtgca gccaaaagtt gatggcgttg cggtaaccct
    16621 ggtttatcgg gacgggaaac tgaacaaagc aatcagtcgc ggtaatggcc tgaaaggcga
    16681 ggactggacg cagaaagtta gcttaatttc cgctgtgccg caaaccgtta gcgggccttt
    16741 agccaacagt acgcttcagg gggaaatatt tctccagcgc gaggggcata tccaacaaca
    16801 aatgggggga ataaatgccc gcgcaaaagt tgctggcctg atgatgcgcc aggacgatag
    16861 cgacacgctg aattctctgg gcgtttttgt ctgggcatgg ccggatggac cgcagttaat
    16921 gaccgatcgt ttaaaggagc tggctaccgc aggttttact ctaacgcaga ggtatacccg
    16981 tgcggttaaa aatgctgatg aggttgcgcg cgtacgcaat gagtggtgga aagcgaaatt
    17041 acccttcgtc accgatggcg tggttgtgcg aggggcgaaa gaacccgaat cccgccattg
    17101 gctaccaggc caggcagaat ggctggtggc ctggaaatat caacctgtag ctcaggttgc
    17161 cgaagtgaag gcgattcagt ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc
    17221 actcgcacct gtcatgctgg atgataaaaa agtccagcgg gtgaatattg gttccgtcag
    17281 gcgctggcag gagtgggata ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca
    17341 gggtattcct cgcattgatg atgtggtctg gcgcggtgca gaacgtacaa aaccgacacc
    17401 accagaaaac cgctttaact cgttgacctg ctactttgct tctgatgttt gtcaggaaca
    17461 gttcatttca cgcttagtct ggctggggtc aaaacaggtt ctggggctgg atggtattgg
    17521 tgaggccggc tggcgcgcgc tgcatcagac tcatcgcttt gagcatatct tttcctggct
    17581 tttattaacg ccagagcaat tacagaacac gccggggatc gcgaaaagta aaagtgcgca
    17641 gctatggcat cggtttaatc tggctcgtaa gcagcctttt actcgctggg tgatggcaat
    17701 gggaataccg ctaacccggg cggcgcttaa tgccagtgat gaacggtcct ggtcgcaact
    17761 tttatttagc acggagcagt tctggcagca gctgccgggg actggatcgg ggcgcgccag
    17821 acaggttatt gaatggaagg aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc
    17881 ccagcagatc acaggttttg aaccttagtg tccttcgtgg ctgtagtaaa agaccggtaa
    17941 tcccagctta aggcgtagcg ccagtaagcg ggcaaaaaag ccgaatacca gggtggagat
    18001 aattaccaca tcatggttag aaacatagtg ttgcagcgcg atgtacagca cggcggaggc
    18061 aaatgagaca ccggcataca actctttctg gaataccagt gggatacgtt tacagaacat
    18121 atcgcgcaaa acgccgccaa acacgccggt ggttaccgcc gcgacaacgg caataattgg
    18181 gccgtgcccc atatccagtg caacctgtgc gccgatgata gaaaagacca ccagtccaag
    18241 cgcatcgagc accagaaaca ctttgcgcag gtaaggcatt acaggagcaa cgatagtggt
    18301 gagtactgcg gcggtagcga cgataatcac atattccggg tgtttgaccc agccgagcgg
    18361 atagtggccc agcagaatat cgcgcactga ccctccgcca attgcggtgg cggtcgcaat
    18421 aataattacg ccaaatgtat ccatgcggcg tcgcccggcc gccagagccc ccgtcatggc
    18481 ttcggcagta atgccaacca aatacaaaat gtgtaacagc atggttctct ccctaaaaat
    18541 ctgtctggca gggtagcgat ttgtgcgtat tgtcacgatt gagatttttt aaggtgagag
    18601 gaaattgata acttgtagtt atgtgaaatc gctattttct gtagcgtaga tgcattcttc
    18661 tgacttcttg tctgagaaaa tgcataagaa aaaataggtt aaaattgagg ggtggaaaag
    18721 aaaggtgcaa tgtttagcca ttgtgttcac cgcacctgga ctttggctta cgcatcgggt
    18781 atctcaatca acaaaaagag tttttagttt ctgctatttc ttctctgtaa tttttacact
    18841 gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac tcctcaggca tggttccgcc
    18901 aagttcctga atggtttgcc tcaccttgct acccacgtca aagtgtgtgg tattagcttg
    18961 ctgttttgaa ttcacgccat cccgcttgag tttttcttct gtttgggtag ctcgaaagag
    19021 attagccgcc agttctgttg aacccatatg atccaggatc ttctgactct ttttcagccc
    19081 cttccgctga tggatggctt tctgatctaa tccaccatac agcccccggt aaccatgatt
    19141 ctgaaatatg gcgaaatcga tagctgtcgc taccccagct tgctgcgctg cctcaaccaa
    19201 ttgtttgtta tgttctttca attcatttct cagaaaaaga cgcttttcgt cttcacgaag
    19261 ttgcctgaaa gcttcatcgt cagccagctc ctgccgtcgg gtctggatag caaaataagt
    19321 ttgccctgcc gcaataaccg gtttcgcagg gtcgccgttt tgtactacca ggtaacaggc
    19381 atagcgggag agatgaacat cttcaagttc tcgttgagcc cctgaaccta atacaaccat
    19441 tttggtggtt tcaacgaaat ggtcagatgc agcttgattg ctggcttcgc acgcttgagt
    19501 cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga taatccagta acggtgccaa
    19561 ttctctagct gaccaaaatt cttgcccttc agtgccataa tgccgtattt cttcaaaagg
    19621 ttgatgatgt tcgttcattg cccactccat gtgatatcga ttgcaaagaa agtaaatctg
    19681 taactgtatt tatatacagt tgtgagtatc tcacgttctc agtgtgctgg gcaaaaaaac
    19741 atcgcaaaat ctggagcagg cacgcaaaac agaattgaag ttgcggggaa gaaaaatgcc
    19801 gctctgtttg agtgggcggc atttgtgaag gctgaaaagt aagaattagt gttacgagtt
    19861 actcaatatt ctgaatctgc tcgcgcatct gctcaatcaa cactttcagc tcaatcgcgg
    19921 agtttgtcac ttcggcattg atagacttcg acgcaagagt gttcgactcg cggttgaatt
    19981 cctgcatcat aaagtccaga cgacgaccaa cggcttcttt tttcttcaga atgttgtagg
    20041 tctctttgac atgcgcttcc aggcggtcca gttcttcggc aacgtcaatt cgttgtgcca
    20101 gcagaaccaa ctcctgctcc aggcggttgt tttccagttg cacttgagca tcttccagct
    20161 tcgcgaccag acgctcacgc tgccattgca ggatttccgg catatgggcg cggactttga
    20221 ccacttcggc ggtgacgcct tccagacgct gctcgatcaa tgctttcagc gcctgacctt
    20281 cggtttcacg tgcgacgata aagtcgtcca gggtaccatc caacgccgcg agaatttcag
    20341 cggcaatcgc gtcgagatcc tgctcctggg ctgccattac gcccggccag cgcagaatat
    20401 caaccgggtt gatttcccct tcgtcactct gcatttttac ccagttcgcg gcagttacca
    20461 gctgtttagc cagtttttcg ttgaggatca gctcaccttg cgcgctaaca tctggctcat
    20521 agcgcagggt acattccact ttaccgcgcg tcaggcgaga acgaatacgc tcgcgaacga
    20581 caggttcaag gctacggaac tgctccggca gacgaaagta agtttccaga taacgctggt
    20641 ttaccgagcg catttcccag gttgcgctcc cccattcacc cttgatttca cgccgggcgt
    20701 aggcggtcat actgcggatc atagacgttc ctgtttataa aaggagaggt ggaaggatta
    20761 tagccatcga tgccttgtca ggataggaat aaccgccgga agtccgtata atgcgcagcc
    20821 acatttgttt caagccggag atttcaatat gcgtccagca ggccgtagca ataatcaggt
    20881 gcgtcccgtt accctgactc gtaactatac aaaacatgca gaaggctcgg tgctggtcga
    20941 atttggcgat accaaagtgt tgtgtaccgc ctctattgaa gaaggcgtgc cgcgcttcct
    21001 gaaaggtcag ggccagggct ggatcaccgc agagtacggc atgctgccac gttctaccca
    21061 cacccgtaac gctcgtgaag cggcgaaagg taagcagggt ggacgcacaa tggaaatcca
    21121 gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg aaagcgctgg gtgagttcac
    21181 cattacgctg gactgcgacg tgcttcaggc tgatggtggc acgcgtaccg cgtcgattac
    21241 gggtgcctgc gtggcgctgg cagatgcgct acagaagctg gtggaaaacg gcaagctgaa
    21301 aaccaacccg atgaaaggga tggtagccgc agtttctgtc ggtattgtaa acggtgaagc
    21361 aatttgcgat ctggaatacg ttgaagactc agccgcagag accgacatga acgtagtgat
    21421 gaccgaagac gggcgcatca ttgaagtgca ggggacggca gaaggcgagc cgttcaccca
    21481 tgaagagcta ctcaccttgt tggcgctggc ccgaggggga atcgaatcca ttgtagcgac
    21541 gcagaaggcg gcgctggcaa actgattttt aaggcgactg atgagtcgcc ttttttttgt
    21601 ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat atcagcgcca gtttattgaa
    21661 tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta cgttgaagtc cgggcgcaaa
    21721 agcccctatt tcttcaacgc cgggctgttt aataccgggc gcgatctggc actgttaggc
    21781 cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt tcgatctgct gtttggccct
    21841 gcttacaaag ggatcccgat tgccaccact accgccgtgg cgctggcgga gcatcatgac
    21901 cttgacctgc cgtactgctt taaccgcaaa gaggcgaaag accacggtga aggcggcaat
    21961 ctggttggta gcgcgttaca aggacgcgta atgctggtag atgatgtgat caccgccgga
    22021 acggcgattc gcgaatcgat ggagattatt caggctaatg gcgcgacgct tgctggcgtg
    22081 ttgatttcgc ttgatcgtca ggaacggggg cgcggcgaga tttcggcgat tcaggaagtt
    22141 gagcgtgatt acaactgcaa agtgatctct atcatcaccc tgaaagacct gattgcttac
    22201 ctggaagaga agccggaaat ggcggaacat ctggcggcgg ttaaggccta tcgcgaagag
    22261 tttggcgttt aaagaaactc gccggatgaa aagtcatccg gcgtcatatt actgcaactg
    22321 tgccgcaatt agcggccagc gggcgtcaaa atcatccgtc gggcggtatt taaattcgct
    22381 gcggacaaaa cgtgacagca taccttcaca gaaggccagg atctggcttg ccagcagggt
    22441 ttcatcggtg gtgtaacctt caccctcacg cattctcttt tcacgcaata cctggcgcag
    22501 ctgcgcttca atacgctcga acagctggtt gatgcgccct tgcaggcgat cctgttcaaa
    22561 cattagcgca tgaccagtga ggatgcgggt caggccagga ttacgctcac caaaaccgag
    22621 aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct ttctcatctt tcagaatcag
    22681 gttgatgcga gtaatcaggc tatcttcgat aaactcaatc aggctatcga acatgcgggt
    22741 cttactgggg aagtggcgat acagtgccgc ttcggaaacg ccgacagagg cggccagttt
    22801 tgccgtcgtg atacgttggc ttccatcgct ggattccagc atcagcgcca gagactgaag
    22861 tatttcctcg cgacggttcc ttttcgcagt ttgtttttct gccatgttac aaaatacccc
    22921 tgaaaataag cacttgccag gcggcaccca cgctatgacc gcaaacgaaa tgtttgcggc
    22981 tatgttatga cgttattcgg atgcgtatgt gttactgacg accagagtga ccaaagccgc
    23041 cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt aaattcagcc tgtactaccg
    23101 gaacaaaaat catctgggcg atgcgttcgc caggttgaat ggtgaagctg tcctgaccac
    23161 ggttccacac ggaaatcatc aactggccct gatagtcaga atcgatcaat cctaccaggt
    23221 taccaagcac gataccgtgc ttatgtccca atccggagcg cggcagcatc attgccgcca
    23281 gtgaaggatc ggcaatatga atcgccagcc cggtcggaac cagcgtagtg tcacccggag
    23341 ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag tccggcagag ccagaggtgg
    23401 cataagtcgg gagcggaaat tccttcccaa cgcgcgggtc cagaatctta acgtcgattt
    23461 ttttcatcat aacgggtcat gatctcgtcg agtaataatt ggccaaggag ctctttgcgc
    23521 tcaagcggta agactttatc tccgtcctgc cagaaaaggt gtaatgcgtt gttgtcgctg
    23581 ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga tcagatcaag gtttttacgg
    23641 atacgttttt gccgggcgta ttcttccaca ttatttgttt cggcggcaaa tccaacgacg
    23701 tagggtcgat ggtcttttag tgcggcaacg cctgcgacga tatcggggtt tttaaccatt
    23761 tttattgtta attcatcacc ctgcgtggcc tgctttttga gtttctctgg ggccacggta
    23821 gctgcgcgat aatccgccac ggcggcgcag ccgataaaaa tattttgctg ctgtacagaa
    23881 gcattcacgg cggcttccat ttccagcgca gtcatcacat caacacgttt aacaaacggt
    23941 ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt tcgcgccacg acgggcagcg
    24001 gcggcggcga tagcaaaacc catcttgccg gagctgtgat tagagatata acgcaccgga
    24061 tcgagcggtt cacgcgtcgg accggcggta atcataatgt tcagatgttt caggtcgttg
    24121 acgggcgaaa aatgcgccac cgccatatcc acaatggtta acggatcgag cattcgccca
    24181 ggaccgatat caccacaagc ctgactgcca ctgtctggcc cccagatgag caaaccacgg
    24241 gaagcaagca cctctaaatt atgctgcgtg gcagcggcac ggtacatctg ctggttcatg
    24301 gcggggagca cggctacagg cgcaggtgta gccagacaaa tcgtcgatac caggtcattc
    24361 gccattccgg cagcaacacg ggcaatcaaa tctgccgtgg caggggcgag aatcactaaa
    24421 tcagcccatt tacccagctc aatatggccc atagcggctt cggctgccgg gtccagcaaa
    24481 ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa gtggggtgat aaaggctttt
    24541 gccgcttcgg tcatggctac gcggacgtcg gccccgcgat cgcgcaaacg acgcaccagt
    24601 tcaggggttt tataggcagc aataccgccg ctaacgccga gaacgatttt tttaccggcc
    24661 aggctcatga tgatttttcc tgttgggtga caccagaagt tggcgatttt atcacaatac
    24721 ttttgttgtc gtgccttcac ctgagaatca ctttgcgagg cgctttccag gattgaaaac
    24781 tggccgtcga tttaacggaa cggctatgac aggatgcgag caccacaaag gaggtgaagg
    24841 tgaaaaacaa tgcacagctg ttgatgccgc gcgaaaaaat gctgaagttt ggtattagcg
    24901 ccttaacgga tgtcgagctg ctggcgttat ttctgcgtac cggaacgcgc ggtaaagatg
    24961 tattaaccct ggcaaaagag atgctggaga atttcggctc tctttatggc ttgttaacct
    25021 ctgaatatga acaatttagt ggcgttcatg gaattggcgt agcgaaattt gcccagttaa
    25081 aggggattgc tgaactggcg cggcgttact acaacgtgcg gatgcgtgaa gaaagccctt
    25141 tactcagccc ggcgatgacg cgggaatttt tacaaagcca gctcacgggt gaggagcggg
    25201 agatctttat ggtgatcttt ctcgactcac aacaccgggt tataacgcat agccgtcttt
    25261 tttccggcac gctaaaccat gttgaagtcc atcctcggga aattatccgc gaagcgataa
    25321 aaataaacgc ctcggcgctg atccttgcac ataatcaccc ttcgggttgt gctgaaccca
    25381 gtaaagcgga taaactcatt actgaacgga taataaagag ttgtcagttc atggatttac
    25441 gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt ttcttttgcc gaacgcggct
    25501 ggatttaacc cgctatgcgc gatccttcgg gatctttgtc tgttcgggac ttgagcacat
    25561 cgctgagtca gcgtatacta cgccaccttt gagaatctcg ggtttggcat ttgggcctgg
    25621 caatcgagag ttcacataga actgcgatga ccgggctgta aagcctgacg aggcgccaat
    25681 accccatacg aagctcgagc taatttgatt tttggagaat agacatgtcc cgagtctgcc
    25741 aagttactgg caagcgtccg gtgaccggta acaaccgttc ccacgcactg aacgcgacta
    25801 aacgccgttt cctgccgaac ctgcactctc accgtttctg ggttgagagc gagaagcgtt
    25861 ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat cgataaaaaa ggcatcgata
    25921 cagttctggc tgaactgcgt gcccgtggcg aaaagtacta agtacttaga ggaaataaat
    25981 catggctaaa ggtattcgtg agaaaatcaa gctggtttct tctgctggta ctggtcactt
    26041 ctataccact acgaagaaca aacgtactaa gccggaaaaa ctggaactga aaaaattcga
    26101 tccagttgtt cgtcagcacg tgatctacaa agaagcgaaa atcaaataat tctcgctttg
    26161 atgtaacaaa aaacctcgct ccggcggggt tttttgttat ctgcttgccc ccatattgat
    26221 tgcatctgtt cattcctgga gatgctatgc ctgaattacc cgaagttgaa accagccgtc
    26281 gcggcataga accgcatctc gttggtgcaa ccattcttca tgcagtggtg cgcaacggac
    26341 gcttgcgctg gccggtttca gaagagatct accgtttaag cgaccaacca gtgcttagcg
    26401 tgcagcggcg ggctaaatat ttgctgctgg agctgcctga gggctggatt atcattcatt
    26461 tggggatgtc tggcagcctg cgcatccttc cagaagaact tccccctgaa aagcatgacc
    26521 atgtggattt ggtgatgagc aacggtaaag tgctgcgcta caccgatccg cgccgctttg
    26581 gtgcctggct gtggaccaaa gagctggaag ggcataatgt gctggcccat cttggaccgg
    26641 agccgctcag cgacgatttc aatggtgagt atctgcatca gaagtgcgcg aagaaaaaaa
    26701 cggcgattaa accgtggctg atggataaca agctggtggt aggggtaggg aatatctatg
    26761 ccagcgaatc actgtttgcg gcggggatcc atccggatcg gctggcgtca tcactgtcgc
    26821 tggcagagtg tgaattgtta gctcgggtga ttaaagcggt gttgctgcgt tcgattgagc
    26881 agggcggtac aacgctgaaa gattttctgc aaagtgatgg taaaccgggc tatttcgcac
    26941 aggaattgca ggtttacggg cgaaaaggcg agccgtgtcg ggtgtgcggt acgccgattg
    27001 tggcgactaa acatgcgcag cgggcaacgt tttattgtcg gcagtgccag aagtaattca
    27061 tgcgcgccgg atggcatacc atccggcata aacgctacgc taacttcgcc atcagcgcct
    27121 gatggacatt ctccggcagg aaatgggtga catcgccctg atggcgcgcc acctctttca
    27181 ccaacgatga agagataaac gaccactctt tcgacggcat cagaaacaca ctttccagtt
    27241 ccggcattaa gtggcgattc atatgcgcca gctgcatttc atattcaaaa tccgccaccg
    27301 cacgcaggcc acgaatcagc accgtagcgt gttgattacg ggcgaagttc gccatcaaat
    27361 cactaaaccc gaccacttcc acgttcccca gatgcgcggt tgcctgctgt gccagcgcca
    27421 cacgctcttc cagggtaaac atcggttttt tactggggct ggcggcaatc gccagaataa
    27481 cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat atgaccattg gtaatgggat
    27541 cgaaagtacc cggataaatc gcccgttttt gcataacaac ctcaatgcgt tttcggtggc
    27601 aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc cctggttttg atacagtact
    27661 tcaacggcat gacggccata gaaactacgg taatcggcgt cggtgagtaa agaggacacc
    27721 tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc cgcttgcctg ctccagccgc
    27781 gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca ataccggaat agcgtgtgcg
    27841 gcagcttcca gcggattatg cccaccacgt tcaaccagtg aaccgccaac aaaggcgaga
    27901 tcggcaatgc cgtacagtaa catcaactcg cccatcgtat cgccaaccac aacctgcgtg
    27961 ctggtggagg ggacttcccc tgaagagcgt gtgatatagc ttagtccagc ctggcggaca
    28021 agattaatcg catccgggaa gcgttctgga tgacggggta ccaggatgag caataaattc
    28081 gggaattgct gtaacaatgc ctgatgtgcg gcgatcacca cactctcttc gccttcgtga
    28141 gtgctggtgg caatccatac cgggcggtgt ggtgcccact ggcggcgcag cgtcacggct
    28201 ttagcagcca actgcggcgt tacagaaata tcgaatttca ggctaccggt aacggtcacc
    28261 tgattatttt ttgcgcccag cgccacaaaa cgtgcaccat cttcttcatt ttgcgcagca
    28321 atcagcgtaa tacgacgcag caagcgacgg acgaatttac ccagtttggc ataacctgcg
    28381 gccgagcggg cagagagtcg cgcgttagcg atcaccagcg gaattttacg tttgtgtagc
    28441 gccgcaatca ggttaggcca tagttcggtt tccataatca acaccagttt ggggtcgact
    28501 ttattcagga aacggttgag cgcatcgggc aggtcatacg gcagataaac gtgctgaaca
    28561 tccttcccga aagccgattg tacgcgctcc gaaccggttg gcgtcatggt tgtaacggta
    28621 atcggtaaat caggataacg atggcgcagc gcgcgcacca acgggatcgc cgccagagtt
    28681 tcaccgacgg agacggagtg cagcataatg ccgcctggtt ttagcggatg gcggtaaaaa
    28741 ccgtaacgtt caccccagcg ttttcgatag gccggagcct tacgtccgcg cacccagagc
    28801 cgtatccaga tcagcggctg aataaggtag agaagggcgg tgtaaagcaa ttcgagcata
    28861 gtaaatagct gacttatgga tgtgctgggg attctatgta tttagctgtg gctttaccat
    28921 tacttttccc gtttttgact taaatagcaa tttacccgcc aattcttgct gaacatctat
    28981 actttttttg tatttttatg cgattcattg atttctgctg tacgtgcagg aaggaatcgc
    29041 agagcaacag tgaaagaata tggttgctaa tgattttatt tgattgattt ctttattgaa
    29101 aatcaacaat gtgcttaaaa atcattcggg tcggattgcc gcgaaagaag tgatacacta
    29161 gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg ttgagcctgg ggcggtagcg
    29221 tgctttttct gcttcacctt aatcggataa tctcaacaaa aagagttact tgtggataag
    29281 ccatttcgaa gaattttgct cattaagatg cgttttcatg gggatatgtt attaactact
    29341 cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt gctgctttat
    29401 caggacacca tcccgatcct gtctgaaaat ccagagatta acgcgctcta cggcataaaa
    29461 aataaaaaag caaaagcctc agaaaaaatt gccaactttt ttcatctcat caaggtatta
    29521 cgtgccaata agtatgacct tatcgtcaat ctcaccgatc aatggatggt tgctatactg
    29581 gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg gcagtctgct
    29641 ttttggcgta aaagtttcac ccatttggtg ccgttgcagg gtggaaatgt ggtggaaagt
    29701 aacttatccg tgctgacccc attgggagtt gattcgttgg tgaagcagac aaccatgagt
    29761 tacccgcctg caagctggaa acgtatgcgt cgcgaacttg atcacgctgg tgttggacaa
    29821 aattatgtgg ttatccaacc tacggcgcgg caaatcttca aatgctggga caacgccaag
    29881 ttttccgctg tgattgatgc cttacatgct cgtggttatg aagtcgttct gacgtccggc
    29941 ccagataaag acgatctggc ctgcgtcaat gaaattgcgc agggatgcca gacgccacca
    30001 gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg gtgcgttaat cgatcatgcg
    30061 cagctgttta ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt taatacgccg
    30121 ctgatatcgc tgtttggtgc gacagatcat attttctggc gtccctggtc aaataacatg
    30181 attcaattct gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg tgaccgaaat
    30241 gagatgtatc tttcggttat tccggcggca gatgtcattg ctgctgtcga taaattactg
    30301 ccctcctcca cgacaggtac gtcgttatga tcgttgcttt ttgtttatat aaatattttc
    30361 cctttggcgg tttgcagcgt gattttatgc gtattgctca gacagtcgcc gcccgaggtc
    30421 atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta tttgaactga
    30481 tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa tgcggagtat tttgcctggg
    30541 tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt tggattcaac aaaatgccgg
    30601 ggctggacgt ttattatgcc gctgatgttt gttatgccga gaaagtagcg caggaaaaag
    30661 gctttttcta tcgcctgacg tcacgttatc gccattatgc cgcctttgag cgggcaacct
    30721 tcgaacaggg taagccgaca cagctgctga tgctgacaga taagcaaatc gccgatttcc
    30781 agaaacatta tcagactgaa gcggagcgtt ttcatattct gccaccgggg atttatcctg
    30841 atcgtaaata tagccagcag ccagccaata gccgtgaaat cttccgtaag aagaatggaa
    30901 taaccgaaca acaatattta ttgttgcagg tcggttcaga cttcacgcgt aaaggtgtcg
    30961 atcgttccat tgaagcactt gcttcgttac cggattcgct gcgccacaac acattgctat
    31021 atgttgttgg gcaggataaa ccgcgaaaat ttgaggcgct ggcagaaaaa cgcggcgtgt
    31081 gcagtaatgt tcactttttc tcggggcgca atgatgtctc ggaattaatg gcggcggcgg
    31141 atttattact gcatcctgcc taccaggaag cggcgggaat tgtgctgctg gaagcgatta
    31201 ctgcaggatt accggtacta acaacagccg tttgtggcta tgcgccttat attgtcgagg
    31261 ctaattgcgg cgaggctatt gctgagccat tccgccagga aacattgaat gagattttac
    31321 gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg cgatattatg
    31381 ctgatacaca agatttatac agtctgccag agaaagcggc ggatatcata acgggtggtc
    31441 tggatggttg aacttaaaga gccgtttgcc acgttatggc gcggcaaaga tccttttgag
    31501 gaagttaaaa ccttgcaggg tgaggtattt cgtgaactgg aaactcgccg tactctgcgc
    31561 tttgaaatgg cgggcaaaag ctattttctc aaatggcatc gcggcacgac cctgaaagag
    31621 ataatcaaaa atttactctc attgcggatg ccagtattag gcgcagaccg cgaatggaat
    31681 gcgattcatc gactgcggga tgtcggcgtt gatactatgc atggggtggc atttggcgaa
    31741 aaaggcatga atccgctgac cagaacttca tttattatta ccgaagatct gacaccaacc
    31801 ataagtctgg aagattactg tgctgactgg gcgactaacc ctccagatgt tcgcgtaaag
    31861 cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata tgcatgctgc gggcattaac
    31921 caccgtgact gttatatctg tcatttcctg ctgcacttgc ctttttccgg taaggaagag
    31981 gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc ttcgcacgcg cgttccacgt
    32041 cgttggcggg ataaagatct tattgggctt tatttttctt cgatgaatat cggcctgact
    32101 cagcgggata tctggcggtt tatgaaagtg tattttgccg ccccgcttaa agacattctc
    32161 aagcaggaac aaggactgct gtcgcaagca gaagcaaaag ccacaaaaat cagggaaaga
    32221 acgattcgaa aatcgttgta acccgtaact tatttttgcc aaaattttgg atacagaata
    32281 aatatgtctc aactcaatga tagtgacatc atcctttttg agtataattt tcattatcaa
    32341 aatataagat ctaaaaatac tcttgatata gcatttggta ttgacagaaa
//
LOCUS       DAZUFB010000048        32223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZUFB010000048 DAZUFB010000000
VERSION     DAZUFB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.048"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..423
                     /locus_tag="ACHY3M_002512"
     CDS             124..423
                     /locus_tag="ACHY3M_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317414.1"
                     /translation="MGNGNFWQLFLGKEPYGKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(470..1352)
                     /gene="rarD"
                     /locus_tag="ACHY3M_002513"
                     /pseudo
     CDS             complement(470..1352)
                     /gene="rarD"
                     /locus_tag="ACHY3M_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003828953.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
     gene            complement(1404..1871)
                     /gene="yigI"
                     /locus_tag="ACHY3M_002514"
     CDS             complement(1404..1871)
                     /gene="yigI"
                     /locus_tag="ACHY3M_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFS4317415.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2036..2905
                     /gene="pldA"
                     /locus_tag="ACHY3M_002515"
     CDS             2036..2905
                     /gene="pldA"
                     /locus_tag="ACHY3M_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFS4317416.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3038..4867
                     /gene="recQ"
                     /locus_tag="ACHY3M_002516"
     CDS             3038..4867
                     /gene="recQ"
                     /locus_tag="ACHY3M_002516"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4317417.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQVNGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4931..5551
                     /gene="rhtC"
                     /locus_tag="ACHY3M_002517"
     CDS             4931..5551
                     /gene="rhtC"
                     /locus_tag="ACHY3M_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFS4317418.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5613..6233)
                     /gene="rhtB"
                     /locus_tag="ACHY3M_002518"
     CDS             complement(5613..6233)
                     /gene="rhtB"
                     /locus_tag="ACHY3M_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFS4317419.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6344..7366
                     /gene="pldB"
                     /locus_tag="ACHY3M_002519"
     CDS             6344..7366
                     /gene="pldB"
                     /locus_tag="ACHY3M_002519"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFS4317420.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7374..8174
                     /gene="yigL"
                     /locus_tag="ACHY3M_002520"
     CDS             7374..8174
                     /gene="yigL"
                     /locus_tag="ACHY3M_002520"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFS4317421.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8250..9149
                     /gene="bioP"
                     /locus_tag="ACHY3M_002521"
     CDS             8250..9149
                     /gene="bioP"
                     /locus_tag="ACHY3M_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFS4317422.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9037..9990)
                     /gene="metR"
                     /locus_tag="ACHY3M_002522"
     CDS             complement(9037..9990)
                     /gene="metR"
                     /locus_tag="ACHY3M_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFS4317423.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10108..12369
                     /gene="metE"
                     /locus_tag="ACHY3M_002523"
     CDS             10108..12369
                     /gene="metE"
                     /locus_tag="ACHY3M_002523"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4317424.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12408..13223)
                     /gene="ysgA"
                     /locus_tag="ACHY3M_002524"
     CDS             complement(12408..13223)
                     /gene="ysgA"
                     /locus_tag="ACHY3M_002524"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFS4317425.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNCGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13485..14246
                     /gene="udp"
                     /locus_tag="ACHY3M_002525"
     CDS             13485..14246
                     /gene="udp"
                     /locus_tag="ACHY3M_002525"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFS4317426.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14387..15814
                     /gene="rmuC"
                     /locus_tag="ACHY3M_002526"
     CDS             14387..15814
                     /gene="rmuC"
                     /locus_tag="ACHY3M_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFS4317427.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15909..16664
                     /gene="ubiE"
                     /locus_tag="ACHY3M_002527"
     CDS             15909..16664
                     /gene="ubiE"
                     /locus_tag="ACHY3M_002527"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFS4317428.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16678..17283
                     /gene="ubiJ"
                     /locus_tag="ACHY3M_002528"
     CDS             16678..17283
                     /gene="ubiJ"
                     /locus_tag="ACHY3M_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFS4317429.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17280..18920
                     /gene="ubiB"
                     /locus_tag="ACHY3M_002529"
     CDS             17280..18920
                     /gene="ubiB"
                     /locus_tag="ACHY3M_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFS4317430.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18999..19268
                     /gene="tatA"
                     /locus_tag="ACHY3M_002530"
     CDS             18999..19268
                     /gene="tatA"
                     /locus_tag="ACHY3M_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFS4317431.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19272..19787
                     /gene="tatB"
                     /locus_tag="ACHY3M_002531"
     CDS             19272..19787
                     /gene="tatB"
                     /locus_tag="ACHY3M_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFS4317432.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19790..20566
                     /gene="tatC"
                     /locus_tag="ACHY3M_002532"
     CDS             19790..20566
                     /gene="tatC"
                     /locus_tag="ACHY3M_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFS4317433.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20608..21390
                     /gene="tatD"
                     /locus_tag="ACHY3M_002533"
     CDS             20608..21390
                     /gene="tatD"
                     /locus_tag="ACHY3M_002533"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFS4317434.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21387..21875)
                     /gene="rfaH"
                     /locus_tag="ACHY3M_002534"
     CDS             complement(21387..21875)
                     /gene="rfaH"
                     /locus_tag="ACHY3M_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFS4317435.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22042..23535
                     /gene="ubiD"
                     /locus_tag="ACHY3M_002535"
     CDS             22042..23535
                     /gene="ubiD"
                     /locus_tag="ACHY3M_002535"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFS4317436.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLC
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23581..24282
                     /gene="fre"
                     /locus_tag="ACHY3M_002536"
     CDS             23581..24282
                     /gene="fre"
                     /locus_tag="ACHY3M_002536"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFS4317437.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24474..25637)
                     /gene="fadA"
                     /locus_tag="ACHY3M_002537"
     CDS             complement(24474..25637)
                     /gene="fadA"
                     /locus_tag="ACHY3M_002537"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFS4317438.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRRLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSTSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERF"
     gene            complement(25647..27836)
                     /gene="fadB"
                     /locus_tag="ACHY3M_002538"
     CDS             complement(25647..27836)
                     /gene="fadB"
                     /locus_tag="ACHY3M_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFS4317439.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28026..29357
                     /gene="pepQ"
                     /locus_tag="ACHY3M_002539"
     CDS             28026..29357
                     /gene="pepQ"
                     /locus_tag="ACHY3M_002539"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFS4317440.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29357..29971
                     /gene="yigZ"
                     /locus_tag="ACHY3M_002540"
     CDS             29357..29971
                     /gene="yigZ"
                     /locus_tag="ACHY3M_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFS4317441.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30010..31461
                     /gene="trkH"
                     /locus_tag="ACHY3M_002541"
     CDS             30010..31461
                     /gene="trkH"
                     /locus_tag="ACHY3M_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFS4317442.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31473..32018
                     /gene="hemG"
                     /locus_tag="ACHY3M_002542"
     CDS             31473..32018
                     /gene="hemG"
                     /locus_tag="ACHY3M_002542"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFS4317443.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7491 a         8161 c         8808 g         7763 t
ORIGIN      
        1 attgataact ataaagtagt aatttagagt gtgtaagttt ttattgatgg ggtatatttt
       61 aattaaatat aattgaaatg tatttttatt ctataaacaa tatcttgggt gaatggattc
      121 tatatgggaa atggtaactt ttggcagttg ttcttgggta aagaacctta cggcaaaaaa
      181 tctgattttg ttaaggcatg gttattgtgg tgggctatta ccatccttgt tctatttatt
      241 ttaagcctga ctacgacatt aaatttaaga agtataggta tagggttaag ttttgggttt
      301 tgtcaaatcc ttggtagata tacgatgtct ctaggatgga gtaagtactg gggagttttg
      361 ggggtatttc ctgtaacaaa tattgttctt tttcttatct taattttttt caaaaaaaga
      421 taaataagtc agtgggtagg ctctttaaac ggcctaccca tgttaattct taactttttc
      481 tacgctgcgt ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggcga
      541 aagtcaccat cttatcgtca cccggttttt caccataaaa cgtcacagcc agcaggaaca
      601 tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga gagacgcaag cgcgtggcag
      661 cggcggtaaa acacaacagc ggtacggtag tgacaatacc ggcggcgatc agcagtaaat
      721 tcagcgacat cgggttttgc cccatatggc tggttgagct gtcggcaata gcaaacaggt
      781 aaattgccgc cacgggcagc agccacatgg tttcgattaa catgccggtt tgcgcttcaa
      841 cggcaatctt cttgcgtacc agaccgtaga aggcaaaact aaatgccagt cccagcgcga
      901 taataggtag cgaaccaaaa gtccacagct ggactaacac gccacatatc gccagaatca
      961 ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt
     1021 tcaccagcgg gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg
     1081 cccagataaa cagtagccag ttgccaccaa tcagcacggc agagactgcc agcataaaaa
     1141 ttttctgtgg cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctgcacc
     1201 accataaaga aaaacgacca gatcacgcga tgcgtcagga tttcatcggc aggcacgtag
     1261 taaatcaact tgaagtacgc tggcgctata ccccaaataa aataagcggc aagagcgagt
     1321 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     1381 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     1441 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     1501 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     1561 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     1621 tggcgggtta aggtacttcc cacgcacacc agacctgcgg cgacatccag cgctgacgca
     1681 ataaccccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcattgg ctgattttta
     1741 aaggccagct gtgcgaactc tttttcgtaa cgctccagct ccatccctaa cgcgcggtta
     1801 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     1861 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     1921 tgttgttggt ttctacttta ggaagggata actaacggct atggagttaa gtatgtaaaa
     1981 tagcccgcag aaaaatattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     2041 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     2101 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     2161 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcattta
     2221 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     2281 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     2341 aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     2401 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     2461 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     2521 cgactctaac gggcgttccg acccgacctc ccgcagctgg aaccgccttt atactcgcct
     2581 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     2641 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     2701 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     2761 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     2821 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtggg
     2881 ggttatgcta aacgatttgt tttgatgaac ggttgagtgg ttggcaaatc tggaacccag
     2941 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     3001 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     3061 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     3121 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc
     3181 caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     3241 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tgcaagtcaa
     3301 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     3361 gacaggctgc cgcaccgggc aaattcgtct gctttatatc gccccggaac gcctgatgct
     3421 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg ttgatgaagc
     3481 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     3541 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     3601 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     3661 tgaccgtccg aatattcgct acatgctgat ggagaagttc aaaccgctcg atcagttgat
     3721 gcgctacgtg caggaacagc gcggtaagtc cggcattatc tactgcaaca gccgggcgaa
     3781 agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc
     3841 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgacctgca
     3901 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     3961 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     4021 gcgtgatggc ctgcccgcgg aagcgatgct gttttacgat ccggccgata tggcgtggct
     4081 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgaac gtcataaact
     4141 caatgcgatg ggcgcttttg ccgaagcgca aacttgccgt cgtctggtac tgctgaacta
     4201 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     4261 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     4321 gcgttttggt atgggctatg tggtggaagt gattcgcggc gctaataacc agcgtatccg
     4381 cgactatggt catgacaaac taaaagtcta cggtatgggc cgtgataaaa gtcatgaaca
     4441 ttgggtgagc gtgatccgcc agctgattca cctcggtctg gtgacgcaaa atattgccca
     4501 gcattctgcc ctacaactga cagaggctgc gcgtccggtg ctgcgcggcg aatcctcttt
     4561 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     4621 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgatagccga
     4681 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     4741 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     4801 ggaacgcttt ggcaaaccgt ttatggcgct gattcgtgcg catgttgatg gcgatgacga
     4861 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     4921 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     4981 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     5041 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgct
     5101 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtggg
     5161 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     5221 ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca gtttcctgaa
     5281 aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct cggtgttctc
     5341 attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcatttttg cgctgatcat
     5401 tgtcgaaacg ctggcgtggt ttaccgtcgt tgccagcctg tttgccctgc cgcaaatgcg
     5461 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     5521 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     5581 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     5641 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     5701 cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat cacaataata
     5761 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     5821 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     5881 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaagg
     5941 tcaattgcac cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     6001 gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     6061 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     6121 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     6181 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     6241 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     6301 ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     6361 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     6421 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     6481 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     6541 ctacgtaaaa tatgcggaac tggcctatga cctgttccat ttggggtttg atgtcttaat
     6601 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     6661 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     6721 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     6781 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     6841 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     6901 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     6961 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     7021 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     7081 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc ttctcttaca
     7141 agctgaagag gaacgcgtgg tggataaccg catgcatgac cgtttttgtg aactccgcac
     7201 cgccgcgggc catcctgtcg aaggaggacg gccgttggta attaaaggtg cttaccatga
     7261 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     7321 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     7381 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     7441 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     7501 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     7561 ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa tctgattttt gctcataacc
     7621 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     7681 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     7741 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc tggattactg gagccggaag
     7801 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     7861 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
     7921 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
     7981 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
     8041 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gaccttcatc
     8101 ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
     8161 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
     8221 gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc acgattctgt
     8281 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
     8341 cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg cgtacccgtg
     8401 gcaatagcct gaaaacggtc ggcctgtata tgttggtggg cgcgatgcag ctcggcgtga
     8461 tgtatatgct gagtttccgc gcgtatctct acctgacggt ctccgaactg ctgctgttca
     8521 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
     8581 gctggggcta tgcctttagc gccttgttgg cggtgattgg tgccgggatt attcgctatg
     8641 atcaggtcac cgaccatttc tggactggct tgctgctggt gcaactctcc aatatcactt
     8701 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccacagcata
     8761 acgcctttgc gtggttctat cttggcgcgt ttctggtggc ggtaattgca tggttcttgc
     8821 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
     8881 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
     8941 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
     9001 ctatctggca ccaacagccg cactggccaa cgttcattac aggcgcgctg gtgatcctgg
     9061 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
     9121 gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta atcggctgac gctgctcgcc
     9181 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
     9241 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
     9301 cgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
     9361 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
     9421 caacagcgtc tcgctggcga gatcttccgg tgtaattcgc gttttcgccg ccagtggatg
     9481 gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat gcaggccact
     9541 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
     9601 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
     9661 attttctaac gcgggcgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
     9721 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctaatttgcg gcagcacctg
     9781 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
     9841 cacaaatagc cggaaaccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
     9901 ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc gcaacgcttg
     9961 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    10021 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    10081 tactaaaaat tccataaggg gcatataatg acaattctta atcacaccct cggtttccct
    10141 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    10201 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    10261 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgttctg
    10321 accaccagtc tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    10381 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    10441 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggaattcgtt
    10501 aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga cgaggcgctg
    10561 gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    10621 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    10681 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    10741 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    10801 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    10861 gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg acctcgtaca tggtaaagat
    10921 gacgttgctg aactgcacaa gcgcctgcct tctgactggt tgctgtctgc gggtctgatc
    10981 aatggtcgta acgtctggcg cgccgatctt accgagaaat atgcgcaaat taaggacatt
    11041 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    11101 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    11161 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    11221 gcagagtgga gcgccccgat tcaggcacgt cgtcactcta cccgcgtaca taatccggcg
    11281 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    11341 gtgcgtgccg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    11401 ggttctttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaaaggcaat
    11461 ctcgacgcca ataactaccg cacgggcatt gcggaacata tcaggcaggc cattgttgaa
    11521 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    11581 gaatactttg gcgagcacct cgacgggttt gtctttacgc aaaacggttg ggtacagagc
    11641 tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg cccggcaccg
    11701 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    11761 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    11821 accatcgcca aacagattgc gctggcgctg cgtgatgagg tggccgatct ggaagcggca
    11881 gggattggta tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc actgcgtcgt
    11941 agcgactggg acgcctatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    12001 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    12061 gattccattg cggcgctgga tgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    12121 gagttgctgg agtcgtttga agagtttgat tatccgaatg aaatcggtcc aggcgtctat
    12181 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccctgct gaagaaagcg
    12241 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccag actgtggcct gaaaacgcgc
    12301 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    12361 ctggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    12421 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    12481 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtagacgata
    12541 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    12601 ggaatgctgt tatcctgacc gccatataag ccgagaaccg gcgcgttaag atcggttgcg
    12661 atatcaacag gttgtttcgg tgaattcagc gacttatcgc ccgtcagttt gccgtaccac
    12721 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    12781 cagcagaatc cggtgatcat taaacgatga acatcgccgc agttgcggga agcccaactg
    12841 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    12901 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    12961 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    13021 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    13081 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    13141 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    13201 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    13261 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    13321 cctgcagaat gaagagtgat ttatgtgatt tgcatcactt ttggtgggta aatttatgca
    13381 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    13441 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    13501 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    13561 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    13621 ctcaccgcga attcaccacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    13681 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    13741 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    13801 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    13861 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    13921 ccattggcgc gacaactcac gttggcgtga cagcttcttc tgataccttc tacccaggtc
    13981 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    14041 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaaccctg ctgaccatgt
    14101 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    14161 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    14221 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    14281 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    14341 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    14401 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    14461 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    14521 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gcgagttact
    14581 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    14641 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    14701 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    14761 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    14821 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    14881 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    14941 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    15001 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    15061 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    15121 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    15181 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    15241 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    15301 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt cactggcgct
    15361 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    15421 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    15481 ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    15541 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    15601 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    15661 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    15721 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    15781 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    15841 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    15901 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    15961 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    16021 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    16081 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    16141 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    16201 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    16261 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    16321 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    16381 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    16441 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    16501 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    16561 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    16621 tgacggcggg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    16681 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    16741 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    16801 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    16861 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    16921 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    16981 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    17041 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    17101 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    17161 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    17221 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    17281 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    17341 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    17401 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    17461 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    17521 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    17581 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    17641 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    17701 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    17761 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    17821 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    17881 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    17941 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    18001 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    18061 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    18121 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    18181 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    18241 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    18301 cagagctaca cgtcgattca ggctgggtgc caccagatac caacgttgaa gagttcgaat
    18361 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    18421 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    18481 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    18541 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    18601 tcggtattcc tgcgctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    18661 tgccagaact gcctgaattg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    18721 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    18781 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    18841 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    18901 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    18961 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    19021 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    19081 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    19141 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    19201 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    19261 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    19321 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    19381 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    19441 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    19501 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    19561 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    19621 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    19681 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    19741 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    19801 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    19861 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgata tctatcacct
    19921 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    19981 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    20041 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    20101 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gtatggcatt
    20161 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    20221 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    20281 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    20341 ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    20401 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    20461 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    20521 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    20581 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    20641 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    20701 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    20761 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    20821 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    20881 tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg cgcttttgtt
    20941 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    21001 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    21061 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    21121 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    21181 ttgattccgg cggagaaatt gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat
    21241 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    21301 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgctaat
    21361 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    21421 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    21481 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    21541 ggataagggg ttgccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatc
    21601 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtggct gacaccgcgg
    21661 gtcgcgttga tagtcgtggt atgaatcact tctggatcaa attcgacaaa caggtagttg
    21721 ggaaacaatg gctcactgac tgcagtacgt tttccacgca cgattttttc cagggtgatc
    21781 atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg ttgaagttgc
    21841 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    21901 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    21961 gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg ccgtataatg
    22021 ggcgtagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcttg
    22081 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    22141 gaaatcactg aaattgctga ccgcactttg tgtgccggtg ggcctgcgct gttgttcgaa
    22201 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    22261 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    22321 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    22381 tttaagcaag tattgaacat gccgacaaag cggctgcgtg gtgcgccctg ccaacaaaaa
    22441 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    22501 gatgccgcgc cgctgattac ctgggggctg acagtgacgc gcggcccgca taaagagcgg
    22561 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    22621 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    22681 cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca cgattctcgg tgcagtcacc
    22741 cccgttccgg atacactttc agagtatgcg tttgccggat tgctgcgcgg taccaagacc
    22801 gaagtggtga agtgtatctc caatgacctt gaagtgcccg ccagtgcgga gattgtgctg
    22861 gaagggtata tcgaacaagg tgaaacggcg ccggaagggc cgtatggcga ccacaccggt
    22921 tactataacg aagtcgatag tttcccggta tttaccgtga cgcatattac ccagcgtgaa
    22981 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    23041 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    23101 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    23161 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    23221 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    23281 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    23341 cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa aatggggctg
    23401 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    23461 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    23521 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    23581 atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac cgtatatcgt
    23641 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    23701 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    23761 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    23821 atcctcaaag atcatcaaat cgtggtcgat attccccacg gagaagcgtg gctgcgcgat
    23881 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    23941 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    24001 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcaccct
    24061 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    24121 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    24181 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    24241 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    24301 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    24361 catccggcaa tcggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    24421 tggtgcagga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaaac
    24481 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    24541 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacag
    24601 cccagcggat gacccagcgc gatcgcacca ccgttgaggt tgatcttctc gtcaatctgc
    24661 tcaatcagtc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcgttcatc
    24721 tcaaacacgc cgatatcgct ggtagaaagc cccgcttttt tcagcgccag cttcgaggcc
    24781 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    24841 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    24901 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    24961 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    25021 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    25081 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    25141 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgctgt taagcccatc
    25201 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    25261 atcggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc atcgccggtc
    25321 ataatcatcc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    25381 gtaaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatgtta
    25441 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    25501 tccagcgccg ggttacgcgc cagcaggcga cgcattaaat gagcggagag atcttctgca
    25561 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    25621 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    25681 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    25741 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    25801 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    25861 accagcgcca tatccgcttc cgccggagtg gcgataatgc cttcctccag acagcgcacc
    25921 acttcgttga ccatcgggat catcatgcgg gcgataatct cctcttcgct gaaatcgcgc
    25981 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    26041 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    26101 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatcct ctgcgggaag
    26161 cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa tgcccacgac atccagcaga
    26221 tatgccggac ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    26281 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    26341 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    26401 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    26461 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgtttcagc
    26521 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    26581 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    26641 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggattgtg
    26701 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
    26761 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    26821 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
    26881 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    26941 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
    27001 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
    27061 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
    27121 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
    27181 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
    27241 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
    27301 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
    27361 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    27421 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    27481 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    27541 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    27601 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    27661 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    27721 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    27781 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    27841 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
    27901 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    27961 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    28021 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
    28081 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
    28141 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    28201 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
    28261 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
    28321 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    28381 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    28441 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
    28501 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    28561 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
    28621 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    28681 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    28741 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcc gctgacctga
    28801 cccgtacctg gtcggcaaaa agtgacaacg attacgcaca gctggtgaaa gacgtaaatg
    28861 atgaacaact ggcgctgatc gcgaccatga aagctggcgt tagctatgtg gattaccaca
    28921 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    28981 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtatcggcc
    29041 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacgc
    29101 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
    29161 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctactggca ccgtggcgtg
    29221 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    29281 gcattcgtat cgaagacaac gtggtgatcc acgaaaataa cgtggaaaac atgacccggg
    29341 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    29401 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    29461 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    29521 gtgggtggcg ggtgcgccgg atgattcaca acagctgggt ttctctgacg acggggagcc
    29581 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg ttggggaaat
    29641 taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    29701 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    29761 attaaccgaa tatactttgc aatgtgaata tcatcagtta accggcattg aagcgttgct
    29821 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    29881 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    29941 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    30001 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    30061 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    30121 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    30181 cgcaaagaga aaggcgaact taaatcccgt gaggggtttc tgatagtggt gctgttctgg
    30241 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    30301 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    30361 gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct gcaatggttt
    30421 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    30481 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    30541 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    30601 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    30661 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga tagcccgact
    30721 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    30781 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    30841 atcggcgtac agtttacgct ggtggttatt tgtaccctcg tactgtggtt tcataatgtc
    30901 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtgtc gatggcgaca
    30961 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    31021 ttatgttcgg cttttatcgg cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc
    31081 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    31141 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    31201 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    31261 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    31321 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    31381 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
    31441 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    31501 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    31561 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
    31621 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    31681 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    31741 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    31801 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    31861 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
    31921 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    31981 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc acaaagaata aaaaatgcgc
    32041 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    32101 actactgaca cggaacaacg gcaaacacgc cgctgggtca gcggggttct cctgagaatt
    32161 ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac
    32221 ccc
//
LOCUS       DAZUFB010000049        31974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZUFB010000049 DAZUFB010000000
VERSION     DAZUFB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.049"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHY3M_002543"
     CDS             <1..759
                     /locus_tag="ACHY3M_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317444.1"
                     /translation="SYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERR
                     LGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGEL
                     LFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLE
                     EEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSM
                     VGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            860..1810
                     /gene="paaX"
                     /locus_tag="ACHY3M_002544"
     CDS             860..1810
                     /gene="paaX"
                     /locus_tag="ACHY3M_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFS4317445.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            1792..2382
                     /gene="paaY"
                     /locus_tag="ACHY3M_002545"
     CDS             1792..2382
                     /gene="paaY"
                     /locus_tag="ACHY3M_002545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFS4317446.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVESNRKRLVFDENLRPKQ"
     gene            2613..3473
                     /gene="pdxI"
                     /locus_tag="ACHY3M_002546"
     CDS             2613..3473
                     /gene="pdxI"
                     /locus_tag="ACHY3M_002546"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFS4317447.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            3537..5843
                     /gene="ydbD"
                     /locus_tag="ACHY3M_002547"
     CDS             3537..5843
                     /gene="ydbD"
                     /locus_tag="ACHY3M_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFS4317448.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            6014..6619
                     /gene="ynbA"
                     /locus_tag="ACHY3M_002548"
     CDS             6014..6619
                     /gene="ynbA"
                     /locus_tag="ACHY3M_002548"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4317449.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            6619..7515
                     /gene="ynbB"
                     /locus_tag="ACHY3M_002549"
     CDS             6619..7515
                     /gene="ynbB"
                     /locus_tag="ACHY3M_002549"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4317450.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            7531..9289
                     /gene="ynbC"
                     /locus_tag="ACHY3M_002550"
                     /pseudo
     CDS             7531..9289
                     /gene="ynbC"
                     /locus_tag="ACHY3M_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            9279..10595
                     /gene="ynbD"
                     /locus_tag="ACHY3M_002551"
     CDS             9279..10595
                     /gene="ynbD"
                     /locus_tag="ACHY3M_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFS4317451.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(10646..11251)
                     /gene="azoR"
                     /locus_tag="ACHY3M_002552"
     CDS             complement(10646..11251)
                     /gene="azoR"
                     /locus_tag="ACHY3M_002552"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFS4317452.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            11452..15354
                     /gene="hrpA"
                     /locus_tag="ACHY3M_002553"
     CDS             11452..15354
                     /gene="hrpA"
                     /locus_tag="ACHY3M_002553"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFS4317453.1"
                     /translation="MTEQQKMTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            15626..16426
                     /gene="ydcF"
                     /locus_tag="ACHY3M_002554"
     CDS             15626..16426
                     /gene="ydcF"
                     /locus_tag="ACHY3M_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFS4317454.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            16623..17925
                     /gene="aldA"
                     /locus_tag="ACHY3M_002555"
                     /pseudo
     CDS             16623..17925
                     /gene="aldA"
                     /locus_tag="ACHY3M_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(17967..18968)
                     /gene="gap"
                     /locus_tag="ACHY3M_002556"
     CDS             complement(17967..18968)
                     /gene="gap"
                     /locus_tag="ACHY3M_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4317455.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            19157..19687
                     /gene="cybB"
                     /locus_tag="ACHY3M_002557"
     CDS             19157..19687
                     /gene="cybB"
                     /locus_tag="ACHY3M_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFS4317456.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            19932..20105
                     /locus_tag="ACHY3M_002558"
     CDS             19932..20105
                     /locus_tag="ACHY3M_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317457.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            20131..20199
                     /gene="yncP"
                     /locus_tag="ACHY3M_002559"
     CDS             20131..20199
                     /gene="yncP"
                     /locus_tag="ACHY3M_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFS4317458.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(20177..20326)
                     /gene="hokB"
                     /locus_tag="ACHY3M_002560"
     CDS             complement(20177..20326)
                     /gene="hokB"
                     /locus_tag="ACHY3M_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFS4317459.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            20725..22365
                     /gene="trg"
                     /locus_tag="ACHY3M_002561"
     CDS             20725..22365
                     /gene="trg"
                     /locus_tag="ACHY3M_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFS4317460.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASATHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(22403..23326)
                     /gene="ydcI"
                     /locus_tag="ACHY3M_002562"
     CDS             complement(22403..23326)
                     /gene="ydcI"
                     /locus_tag="ACHY3M_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4317461.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELELLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            23543..24886
                     /gene="ydcJ"
                     /locus_tag="ACHY3M_002563"
     CDS             23543..24886
                     /gene="ydcJ"
                     /locus_tag="ACHY3M_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4317462.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            25111..26766
                     /gene="opgD"
                     /locus_tag="ACHY3M_002564"
                     /gene_synonym="mdoD"
     CDS             25111..26766
                     /gene="opgD"
                     /locus_tag="ACHY3M_002564"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFS4317463.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            26906..27130
                     /gene="ydcH"
                     /locus_tag="ACHY3M_002565"
     CDS             26906..27130
                     /gene="ydcH"
                     /locus_tag="ACHY3M_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFS4317464.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            27193..27729
                     /gene="rimL"
                     /locus_tag="ACHY3M_002566"
     CDS             27193..27729
                     /gene="rimL"
                     /locus_tag="ACHY3M_002566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFS4317465.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(27724..28703)
                     /gene="ydcK"
                     /locus_tag="ACHY3M_002567"
                     /pseudo
     CDS             complement(27724..28703)
                     /gene="ydcK"
                     /locus_tag="ACHY3M_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            28827..29819
                     /gene="tehA"
                     /locus_tag="ACHY3M_002568"
     CDS             28827..29819
                     /gene="tehA"
                     /locus_tag="ACHY3M_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFS4317466.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            29816..30409
                     /gene="tehB"
                     /locus_tag="ACHY3M_002569"
     CDS             29816..30409
                     /gene="tehB"
                     /locus_tag="ACHY3M_002569"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFS4317467.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            30712..31380
                     /gene="ydcL"
                     /locus_tag="ACHY3M_002570"
     CDS             30712..31380
                     /gene="ydcL"
                     /locus_tag="ACHY3M_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFS4317468.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(31398..31899)
                     /gene="tnpA"
                     /locus_tag="ACHY3M_002571"
                     /pseudo
     CDS             complement(31398..31899)
                     /gene="tnpA"
                     /locus_tag="ACHY3M_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31907..>31974
                     /locus_tag="ACHY3M_002572"
     CDS             31907..>31974
                     /locus_tag="ACHY3M_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317469.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRR"
BASE COUNT         7995 a         7337 c         8261 g         8381 t
ORIGIN      
        1 tcttattgcc ttaacctgat tgaagagctg gagcggcagt tgggcggtga tgccagcggt
       61 tgctcgctgc gggttggagt gtttggtgct gagccgtgga cacaggccat gcgtaaagag
      121 attgaacggc gtctggggat caccgcactg gatatttatg gcctgtcaga agtgatgggg
      181 ccgggggtgg cgatggagtg tctggaaact accgacggcc caaccatttg ggaagatcat
      241 ttctatcctg aaattgttaa tcctcatgac ggcacaccgc ttgccgatgg tgaacatggc
      301 gaactgttat tcaccacgct gaccaaagaa gcattgccgg tcattcgtta ccgcacgcgt
      361 gatttaaccc gactgttacc aggaacggcg cggactatgc gccgcatgga tcgcatcagc
      421 ggacgcagcg atgacatgtt gatcattcgc ggtgtaaatg tcttcccgtc acaactggaa
      481 gaagagattg tcaaattcga acatttatcg ccccattacc aactggaggt gaaccgccgg
      541 gggcatcttg attcactttc tgtgaaagtg gagttgaaag aaagcagcct gacattgacg
      601 catgagcagc gttgccaggt gtgccaccag ctgcgccatc ggattaagtc gatggtgggg
      661 atctctaccg atgtgatgat cgttaactgt ggcagtatcc cgcgttcaga aggcaaggcg
      721 tgtcgggtgt ttgatctgcg caatattgtt ggtgcctgac gtgtcgtctg gccctggtga
      781 ggtaaagcgc cagggccaga agtcgatacg acctgtgcta tgattcataa atcacaacaa
      841 taacaacaga ctgaatcgaa tgagtaaact tgttactttt atccaacatg ctgtaaacgc
      901 tgttccggtc agtggcacat ctttgatctc ctctctgtat ggtgattcgc tttcccatcg
      961 tggtggtgaa atctggctgg gtagtctggc tgctttgctg gaagggctgg gatttggtga
     1021 gcgtttcgtg cgcaccgctt tgtttcgtct taataaagaa ggctggctgg atgtttcccg
     1081 catcgggcga cgcagtttct atagcctcag tgataaaggc ttgcgcctga cgcgacgggc
     1141 agaaagtaaa atttatcgcg cagagcaacc tgcatgggat ggtaaatggc tcctgttgct
     1201 ctcggaaggt ttagataaat caacgctggc tgatgtcaaa aagcagttga tctggcaagg
     1261 ttttggcgca ctggcaccca gcctgatggc atcgccgtcg caaaaactgg cggatgtaca
     1321 gacacttttg catgaagcgg gggtggcgga taacgtgatt tgttttgaag cgcaaatacc
     1381 actggcgctt tctcgtgcag cactgcgtgc cagagtggaa gagtgctggc atttaactga
     1441 acaaaatgcc atgtacgaaa cctttattca gtcattccgc ccgctggtgc cgctgttaaa
     1501 agaggcagca gacgagttaa ccccggagcg tgcatttcat attcagcttt tactgatcca
     1561 tttttatcgc cgtgtcgtcc ttaaagaccc attgttgcca gaggagttgc ttccggcgca
     1621 ctgggcaggg catacggcgc gtcagctgtg tatcaacatt tatcagcgcg tagcgcctgc
     1681 tgctttagcg ttcgttagtg aaaaaggtga aacctcggtc ggtgaactgc ctgcgccggg
     1741 aagcctgtat tttcaacgtt ttggcggttt gaatattgaa caggaggcga tatgccaatt
     1801 taccagatag atggtctgac tccggttgtg ccagaagaga gttttgtcca tccgacagcg
     1861 gtattgatcg gcgatgttat tctaggcaag ggcgtttacg ttgggccaaa tgccagcctg
     1921 cgtggcgatt ttggtcgtat cgtggtgaaa gatggcgcga acattcagga taattgcgtt
     1981 atgcacggtt ttcccgagca ggatactgtc gtggaagaag atggacatat tggtcatagc
     2041 gctatccttc atggttgcat tatccgccgc aatgcattag tgggaatgaa cgcggtagtg
     2101 atggacggcg cggtgattgg cgagaacagc attgttggcg catccgcatt tgtgaaagcc
     2161 aaagcagaaa tgcctgccaa ttacctgatt gtcggcagcc cggcgaaagc gattcgtgaa
     2221 ctcagtgagc aggagctggc atggaaaaag cagggtacgc atgagtatca ggtgctggtg
     2281 actcgttgta agcagacgtt gcatcaagtt gagccattgc gggaagttga atcgaacagg
     2341 aaacgtctgg tatttgatga gaatctgcga ccgaaacagt aacgtccaga tactgaacat
     2401 tctaattttt agcatgtgat acctaaaccg caacgctgat acaggttgcg gtttttttat
     2461 tgccggatgc ggcgctttac gcgttttgtt ttgtgttttt caggacaata gagcaactca
     2521 tccagtaatc ttgtttacgc cctcgcatta tttatctctt ttcgtttcta tactgatttt
     2581 tcttaatccg ttttattaca gggcagggtg cgatgagcag caatacattt actctcggta
     2641 caaaatccgt taaccgtctt ggttatggcg cgatgcaact ggcaggtcct ggggtttttg
     2701 gtcccccaag agatcgccac gtcgctataa ctgtgctgcg cgaggcgctg gcattgggcg
     2761 tcaatcacat tgataccagc gacttttatg gtccgcacgt caccaatcag attatccgcg
     2821 aagcgcttta tccttactct gacgacctga caattgtcac taaaattggt gcgcggcgtg
     2881 gagaggacgc ttcctggttg cccgcctttt ctccggcaga gttgcaaaaa gcggtgcacg
     2941 ataatctacg taatctcggc ctggacgtac tggatgtggt taacctgcgc gttatgatgg
     3001 gagatggtca tggcccagcg gaaggatcga ttgaggccag cctgaccgtg ctggcagaga
     3061 tgcaacaaca aggcctggta aaacatattg gcctgagcaa cgtcacaccg acgcaggttg
     3121 cagaggcgcg caagattgcc gaaattgtct gtgtgcaaaa cgaatacaac atcgcgcacc
     3181 gtgctgatga tgcaatgatt gatgctttgg cccacgatgg cattgcctac gtgccgttct
     3241 tcccgctcgg gggctttaca ccgctgcaat cctctacgct gtcggatgtt gctgcgagcc
     3301 tgggtgcaac acccatgcag gtggcgctgg cgtggctgtt acaacgttca ccgaatattt
     3361 tgctgatccc agggacgtct tctgttgcgc atttacggga gaatatggct gctgaaaaat
     3421 tgcatctttc tgaggaagtg ttgtctacgt tggatggtat ttcgcgagaa taacgaatat
     3481 acaaaaggga aagatgcatt tcccttttgt ttctttttta atggcatgga gtgcatatgt
     3541 ttaagtccag gatttgcggc tggataggtt tacttcccct gtttatgctg agtcttccag
     3601 tacaggcgga acttcgatgt gttgcaaata cagttgatat tgaaccattt ttttctgcgg
     3661 ctaccgctga agataaacaa caagttgaac aagctatcaa cagtagcgtg aatcttgtcc
     3721 ccttcggttt atctgcatcg gactggaaag tgcatcgtgg cgatttagtg gtagaaggta
     3781 atatagagag taatcaaaaa ttgattgttc ttggtaattt gacagtcaaa ggtaatattt
     3841 ccactttctc tctttctaat ccatgggtta ttctcggtaa cgtgacggca acgaatatag
     3901 ttacggattc accactgtta attaccggtt cgataaatgc aagtggactg gtatttatcg
     3961 actcatatta cgataatccg tcttcgatta aggggagtat taatgcgcgt ggaatattta
     4021 tcaatgacat aattgcacct gttgttgcgt cttcgacaaa tagtgaattc atggtccgtg
     4081 cgagtgacaa aaatgacact gaaaatgtca aaaaagcgct gatgataata aatcctgatg
     4141 catattattg ggggctaata aatgatgaag atgctctgaa agaaattttt aagcgaagca
     4201 atattcgcat ggcagggaat gtctgtaatc agatgaaaaa agaagcgctg tttcgcccta
     4261 agccttctcc tgagttagtg caggaattgc aaatgctgga tgaaggcaac gttgctgcat
     4321 ttgaaggacg agatattgcg acatttgatc ttgccataat gcgaactctc ccaaggctta
     4381 aaggaatttc agctaaccta cgcaaacaac ttattaatag caatgatgaa caaacgattg
     4441 aaagtatggc gagatatatg cctgacaatg agattctgga gttgaccgat cagcaattgg
     4501 gttaccagcc tgttgttctg gggttgctcg accgtgaacc gctctctgtc gaaataatga
     4561 cgcgaatgag tcgtctacct gatggtgtcg gtccgttgaa tcttgcgcta cgtgaaaatc
     4621 tccctctgga tatcgtaatg actctggcga aaagagattg ggatatgatc attcaggagc
     4681 tttataaaga tgcgtggtta ttgcctgaat ccattattga tggctatatc cgcagtgatg
     4741 attcctctat tcgtcaggtc ggtgctggag gacaactcac ctacaatcag gcaatgcagt
     4801 tggcgaacga ttcatcaaac aatgttgtca caagcttagc gttcaagctg gcagagatga
     4861 aacaccatgg tcaattattg cggatgacgc cacaagagag tgacaaagtt gcagcctatt
     4921 tataccaaaa attcgagaat gacgatgatc taatacgtgt gttattttta gcattgccag
     4981 ataacttaca gtttaatttt gttaaaagga tggagaaaaa atcccctgcc tacttttgct
     5041 gtcgggatat gcaggtaatt cactctgacg ctgctctaca acggttattg acacgtttca
     5101 acgatcctga ggggtggagt aatctggcga aaaatcagta tctgagcacg tcgatgaaac
     5161 aaaaaatttg gcaacgtgct ttatcgcatc ggaaaaataa tccgaaagca gattcagctg
     5221 cgtatgaaac cagcgctgat atgattttgt ctgagcttat cagccacggt gaagttgatg
     5281 atcagatgct attaaatgcc accgccctga tacgtttgga agactgggac tttttagaga
     5341 gtgcattagt tagttgggat aatttacccg ctgttgtcct caaggaatta cagcaaaata
     5401 cgccacgcaa tgatatttgg gcgaagtttt ttctgaggca ggaaaacagc tcccgtgcgc
     5461 aggttgatga agcattacgc gtctattatg cactcgaccc cgatgcgtta gcacaactgg
     5521 atgtactggc aaaacaaccg gatcgtatat ggtggagtac actggcgaaa agcaatctca
     5581 catttttcaa gttcggcgca cttaacaacc gccacacacc gcctgcagta ctggcggcag
     5641 aaattgatcc cgagtggtgg attgtggcga tgaataatcc ccgttttcca gttgatgtat
     5701 taaaggcgag gctgaaacgt gatcctttgc tggcgttaga gcttgttaac cctgaactgg
     5761 atttagtccg ccagctggcg cttaacggta agacgcgcgc aatacgggaa caagcgatga
     5821 gaaaacttga tgagttgtat tgatccacaa cgtaatattt cttaaccctt tgattaattg
     5881 gggataatta ttcctgacag tgagtcccca ataccttgat atattctgaa tttttaatga
     5941 agcggcgtgt tgcgatatct ccgtcagggg aattgatgca ccatagcgca aaccgaatta
     6001 tcaaggattg ataatgacgc tctaccagat aaaaccgctc tttcagtcgc tgttaaggcc
     6061 gacgatgttt tggctttaca agcaccacgt tacagcgaat cacatcactc ttgctgcact
     6121 ggcgctttct cttcttaccg gattgctgtt gatgttggtg gcacaaccca tcctctttat
     6181 gctattgccc atcgtgcttt ttatccgtat ggcgctcaat gcgctggatg gcatgttggc
     6241 gcgtgagtgc aatcagcaaa cacggcttgg ggcaattttg aatgagactg gtgatgttat
     6301 ttccgatatt gcgctctatt taccattttt atttttaccg gaaagtaacg catcactcgt
     6361 gatactcatg ttattttgca ccatattgac cgagttttgc ggtttactcg cgcagacgat
     6421 taacggtgtt cgcagttatg ccggtccatt tggcaaaagc gatcgcgcgt taatatttgg
     6481 tctgtggggt ctggccgttg ccatttatcc acagtggatg cagtggaata atcttttatg
     6541 gagcattgct tcaattctgc ttctctggac tgcgattaat cgttgtcgga gtgtgcttct
     6601 tatgagcgct gaaagataat gctggaaaaa tctctggcaa cacttttcgc gttgttaatt
     6661 ttagcaacgc tgataaacca ttttctgcta tggcggttac cggagagaaa agaggatgaa
     6721 gttacattac gtattcgtac ctggtggggc atcgtcattt gtttttcact ggtgatttca
     6781 ggtccacgct ggatgacgtt gacgtttttt gcgctgataa gttttctggc attgaaagaa
     6841 tattgtacgc ttatatctgt acattttccg cggtggttat attgggttat tcctcttaac
     6901 tatttgttta tcggttttaa ttgctttgag ctgtatctat tatttatacc tttggctggg
     6961 tttctaatat tagccaccgg gcaagttttt atgggggacc cctccggttt tctgcatacc
     7021 gtgagcgcca ttttttgggg ctggataatg accgttttcg ccttgagtca tgccgcctgg
     7081 ttattaatgt tgccaaccac aaatatccag ggcggggcgt tactggtgtt atttcttttg
     7141 gcattaaccg agtcaaacga tattgcacag tatttatggg ggaaatcctg cggcagaaga
     7201 aaagtggtcc ccaaagtcag cccgggaaaa acgctggaag gtctggtggg gggcgtaatc
     7261 accaccatga ttgcgtcact gattattgga ccgttgctga caccgctaaa tacattacag
     7321 gcattattag cgggtttgtt aattggtatt agtggttttt gcggcgatgt cgtgatgtca
     7381 gccatcaaac gagatattgg tgttaaagat agtgggaaac tattaccagg acatggcgga
     7441 ctccttgaca ggattgactc attaattttc accgctccgg tattttttta ttttattcgc
     7501 tactgctgtt actgaaggaa aatagagaaa atggaaaatt cacgcatcca tggggaacat
     7561 ttttttacca gcagtgataa tacagcgttg ttttatcggc actggcccgc tttacagccc
     7621 ggggctaaaa aggtcatcgt cttatttcat cgcggtcatg aacattctgg tcgtctacaa
     7681 catctcgttg atgaactggc gatgccagat actgtttttt atgcctggga tgcccgaggg
     7741 catggacaaa acttcggggc cgcgtggtta tagcccatct cttgcgcgtt cagtgcagga
     7801 tgtcgatgaa tttgttcgtt ttgctgccag cgatagcctg gtcggactgg atgaggtggt
     7861 agtgatcgcg caaagcgtcg gcgcagtgct ggttgccaca tgggttcatg attatgcgcc
     7921 tgcaattcgc gggctggtgc tggcttctcc ggcctttaag gttaaattgt atgtaccgct
     7981 ggcacgtcct gcgctggcgt tatggcatcg tctgcgtggt ctgtttttta ttaattccta
     8041 tgtgaaagga cgctatttga cccacgatcg gcaacgggtg gcgagtttca ataatgatcc
     8101 gctgatcaca cgggcgattg ccgttaatat cttgctcgat ctctacaaaa cgtctgaacg
     8161 tattattaga gatgcggcgg cgattacgct ccccacgcaa cttctgatat caggcgatga
     8221 ctatgtggtg catcgccaac cgcagattga tttttatcag agattacgta gccctctgaa
     8281 agagctgcat ctgctgccag gcttttatca cgacacgttg ggtgaagaga acagggcgca
     8341 ggcatttgaa aaaatgcaaa gctttattag tcgtttatat gctaacaagt cacaaaaatt
     8401 tgattatcag catgaagacc gcactggacc atcagcggat cgctggcggc tcctttcagg
     8461 tggacccgtg ccattatcgc cggttgattt ggcgtatcgc tttatgcgaa aggcgatgaa
     8521 attgttcggg gcgcactctg cgggcctgca tctcggaatg agcaccggct ttgattcagg
     8581 cagttccctg gattatgtct atcaaaatca accgcaaggc agtaacgcat tcgggcgctt
     8641 aattgacaaa atctacctga acagtgttgg ctggcgcggt attcgccagc gtaaaaccca
     8701 tttacaaata ctgattaaac aagccgttgc cgatctccac gccaaaggtt tagccgtccg
     8761 cgtggttgac attgccgcag ggcatgggcg ctatgtactg gatgcgctgg caaacgagcc
     8821 tgccgtaagc gatattttgt tacgtgatta cagtgagtta aatgttgcac aggggcaagc
     8881 gatgattgcc caacggggaa tgtctgggcg ggtgcgtttt gaacagggcg atgcgtttaa
     8941 cccggaggaa ctcagcgcgt taactccgcg gcctacgctg gcgattgtct ctggcctgta
     9001 tgagcttttt cccgaaaatg agcaggtaaa aaactcactc gcaggtcttg ccaatgccat
     9061 cgaaccgggc ggcattctca tctacaccgg gcagccgtgg caccctcaac tggagatgat
     9121 tgccggggtg ttaaccagtc ataaagatgg taaaccgtgg gtaatgcgcg tgcgttcgca
     9181 aggggagatg gattcactcg tgcgtgatgc cggatttgat aaatgcacac aacggattga
     9241 tgagtggggt atttttacgg tttcgatggc ggtgcgtcgt gataactgaa cgtcggaacg
     9301 tattgctaca aggcgctggc tggttattgt tgctggcccc gtttttcttc ttcacctatg
     9361 gatctcttaa tcagttcacc gcggttcagg accttaacag ccatgatatc cccagtcagg
     9421 tattcggttg ggaaacggcg atcccttttc ttccctggac tattgttcct tactggagtc
     9481 tggatctttt atatggattt tcgctgttcg tttgtagcac gacattcgaa cagcgccgac
     9541 ttgtccaccg gcttattctg gcaacggtaa tggcctgctg cggttttttg ctctatccgc
     9601 tgaagtttag ttttatccgt cctgaagtga gtggggtgac gggatggcta ttttcgcaac
     9661 ttgaactgtt tgatctgcct tataaccagt ctccttcgct gcatattatt ctctgctggc
     9721 tactttggcg tcactttcgt cagcatctgg ctgagaggtg gcgtaaagtc tgtggcggat
     9781 ggtttttact catcgctatt tctactctga cgacctggca gcatcatttt attgatgtca
     9841 tcacagggct ggcggtaggt atgttgattg actggatggt gcccgtcgat cgtcgttgga
     9901 attatcagaa acctgatcaa cgtcgaatca aaatagcact gccatatgtc gtaggcgcgg
     9961 gctcgtgcat tgtgttgatg gagctaatgg tgatgattca gttatggtgg tcagtctggt
    10021 tatgttggcc agtattatcg ctactcatta ttggccgtgg gtacggtggg cttggcgcga
    10081 taacaacagg gaaagatagt caggggaaac tcccgcccgc cgtttactgg ctgacattgc
    10141 cctggcgcat cgggatgtgg ctgtctatgc gttggttttg tcgtcgcctg gagccggtga
    10201 gcaaaattac tgctggtgtt tatttagggg cgtttccacg acatattccg gcacagaatg
    10261 cggttctgga cgtcaccttt gaattccctc ggggacgagc gacaaaagat cgactctatt
    10321 tctgtgtacc gatgctggat ctggtggttc cggaagaggg ggagctccga caggccgtgg
    10381 cgatgctgga aacattacgc gaagagcaag gcagcgttct ggtccattgc gcgttgggat
    10441 tatcgcgcag tgcgctggta gtggcggcat ggttgttatg ttacggacac tgtaaaaccg
    10501 ttgatgaagc gattagttat attcgagcca gacgttcgca tattgtgctt aaggaagatc
    10561 acaaagcgat gctgaaatta tgggaaaaca ggtaagtgga ttgagatgtg gactgaacta
    10621 cagtccacat caagatcgtg tccggttatg cagcaacaat gctgtcgatg gccgctttcg
    10681 cgtcagactg cgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa
    10741 atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt
    10801 ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg
    10861 ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca
    10921 ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa
    10981 cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct
    11041 gctgacgcgg agtcagcggc gcatcgctcg gacgcagtgc accaaccagt tcgccatcca
    11101 gtaccggaat cggatttgca gccaggtcgc gaacggtgat ttcatcagcg gagtgctttt
    11161 cgcgccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
    11221 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatgggc
    11281 gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa
    11341 tcgaagccta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat
    11401 atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt tatgacagaa
    11461 caacaaaaaa tgacctttac ggccttgcag cagcggctgg attcgctgat gctgcgcgac
    11521 agactgcgtt tttctcggcg tctgcacggt gttaagaagg ttaaaaatcc tgatgcacaa
    11581 caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg
    11641 cgtgaagcgg cacgaccgga aattacttat cctgacaatt taccggttag tcagaaaaaa
    11701 caggacattc tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaacgggt
    11761 tctggtaaaa cgactcagtt accgaaaatc tgtatggagc tggggcgcgg gattaaaggg
    11821 ctgatcggcc atacccagcc gcgtcgcctg gcggcaagaa cggtggcgaa ccgtattgcg
    11881 gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
    11941 gttagtgata acacgatggt caagctgatg accgacggta tcctgctggc ggagatccag
    12001 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcaca cgaacgcagc
    12061 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
    12121 aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
    12181 gcgccgatta tcgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgaccgatt
    12241 gttgaagaag ccgacgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac
    12301 gaactgagtc aggaaagccc tggcgacatt ctgatcttta tgagtggtga gcgggaaatt
    12361 cgcgataccg ccgatgcgct gaacaagcta aatctgcgtc ataccgaaat cttgccgctt
    12421 tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggcgc
    12481 attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtt
    12541 atcgaccccg gtacagcacg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
    12601 ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tgggcgtgtg
    12661 tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctttcgcg cccggagttt
    12721 accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg
    12781 gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag
    12841 gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc
    12901 tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgatcc acgtctggcg
    12961 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc
    13021 gcgctttcca ttcaggatcc gcgcgagcgt ccgatggata aacagcaggc atcggacgaa
    13081 aaacatcgtc gcttccacga caaagagtct gacttcctcg cgtttgtgaa tctgtggaat
    13141 tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc
    13201 gattatctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
    13261 gtggtgaaag aacttggcat tccggttaac agcgaaccgg cggagtatcg cgaaattcac
    13321 attgcgttgc tgacgggttt actttcccat atcggcatga aagatgccga taaacaagaa
    13381 tataccggcg cacgtaacgc gcgtttctcc atctttcccg gttctggttt attcaaaaaa
    13441 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
    13501 gctgcgcgta tcgacccgga atgggtggag cctgtcgctc agcatttgat taaacgcacc
    13561 tacagcgaac ctcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
    13621 gtttatggtt tgccgattgt tgccgcgcgc aaggtcaact acagccagat cgatccggcg
    13681 ttatgccgtg aactctttat tcgccatgcg ctggtggaag gtgactggca gacgcgtcac
    13741 gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca
    13801 cgtcgccgcg atattctggt tgatgacgaa acgttgtttg agttctacga ccagcgcatc
    13861 agccacgatg taatctccgc tcgtcacttc gacagctggt ggaaaaaagt cagccgcgaa
    13921 acccctgatt tgctcaactt tgaaaaaagc atgttgatca aagagggcgc agaaaaaatc
    13981 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat
    14041 cagtttgagc ccggcgcgga tgctgacggt gtgaccgtac atattccgct gccgttactt
    14101 aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaactggtg
    14161 attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtacc cgcgccaaac
    14221 tatgccgaag cgtttttagg ccgcgtcaaa ccgctggagt taccgttgct cgacagcctt
    14281 gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat
    14341 caggtgcctg atcacctgaa aattaccttc cgcgtggtgg atgacaaaaa caagaagcta
    14401 aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg
    14461 ctatctgcgg tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt
    14521 cagctgccgg aaagctacga acagaagcgt ggcaactaca aagtgaaggc gtggccggcg
    14581 ctggtggatg agcgcgacag tgtggcgatc aaactgtttg ataacccgct ggagcaaaag
    14641 caggcaatgt ggaacggtct tcgccgtcta ctgctgctga atattccatc gccaatcaaa
    14701 tatttacatg aaaagttacc gaacaaagcc aagctgggac tgtactttaa cccgtatggc
    14761 aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaatt gatcgacgcc
    14821 aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc
    14881 gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg
    14941 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct
    15001 gacattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc
    15061 aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacggct ggaaaaactg
    15121 gcggttgatc cacatcgcga ccgtgctcag atgctgaaag tcgaaaacgt ccagcaggcg
    15181 tggcagcaat ggatcaacaa actgccgccc gcacgtcgtg aggatgaaga cgtgaaagag
    15241 atccgttgga tgatagaaga gttgcgcgtt agttacttcg ctcaacaact tggtacgcct
    15301 tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg ttaaccctgc
    15361 tatttgcctg ataaagaaaa aacccggtaa gcatttagcg ccgggttttt tattaattct
    15421 aaaacggtaa gggtaaaaat tcaggaattc agaaaaatac aattctctgc tgcaagatga
    15481 ataatgttta tctacagcat ttccttaaaa gatatgtcag gcttgcggag tggcggttaa
    15541 ggacatacga cttcctcctt tcagagtgct ccgcttctca ctattatctc acgcagtatt
    15601 cttaagggaa cgataaggag gaaccatgaa cattaccccg tttccgacgc tttcgccggc
    15661 aactatagat gccataaatg ttatcggaca gtggctggcg caggatgatt tctccggtga
    15721 ggtgccgtat caggccgatt gcgtgatcct tgcaggcaat gcggttatgc cgactatcga
    15781 cgcggcatgt aagattgctc gcgatcagca aattccttta ctgattagtg gtggtatcgg
    15841 tcactcgaca acttttttgt atagcgccat cgcacagcat ccgcactaca acactatccg
    15901 caccactggc agagcagaag cgaccatcct ggcggatatc gctcatcagt tctggcacat
    15961 tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa acgcacgctt
    16021 tagcatcgcg ctattgaatc aggccgtaga acgagttcat acggctatcg ttgttcagga
    16081 ccccaccatg cagcggcgca cgatggcgac gttccgccgt atgactgggg acaatcccga
    16141 tgcaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata acgcagacag
    16201 tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc tctcactact
    16261 cactggcgag ctgccgcgtt tacgcgatga tagcgatggc tacggtcccc gcgggcgaga
    16321 ttttatcgtt cacgttgatt ttccggcaga agtcatccat gcatggcaaa cgctgaaaca
    16381 tgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat tgcccgtttg
    16441 tgaaccactt gtttgcaaac gggcatgact cctgactttt atttctgcct tttattcctt
    16501 ttacacttgt ttttatgaag cccttcacag aattgtcctt tcacgatgcc gtctctctga
    16561 tgattgatgt taattaacaa tgcattcacc gaaaacaaac atataaatca caggagtcgc
    16621 ccatgtcagt acccgttcaa catcctatgt atatcgatgg acagtttgtt acctggcgtg
    16681 gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc cgcatacccg
    16741 atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat
    16801 gggaagcgtt gcctgctatt gaacgcgcca gttggttgcg caaaatctcc gccggcatcc
    16861 gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag atccagcagc
    16921 tggctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg gagtgggcac
    16981 ggcgttacga gggcgagatt attcaaagcg atcgtccagg agaaaatatt cttttgttta
    17041 aacgtgcgct tggtgtgact accggcattc tgccgtggaa cttcccgttc ttcctcattg
    17101 cccgcaaaat ggctcccgct cttttgaccg gtaataccat cgtcattaaa cccagtgaat
    17161 ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc cttccgcgcg
    17221 gcgtgtttaa ccttgtactg gggcgtggtg aaaccgttgg gcaagaactg gcgggtaacc
    17281 caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag attatggcga
    17341 ctgcggcgaa aaacatcacc aaagtgtgcc tggaattggg tggtaaagca ccagctatcg
    17401 taatggacga tgccgatctt gaactggcag tcaaagccat cgttgattca cgcgtcatta
    17461 atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc atttatgatc
    17521 agttcgtcaa tcggctgggt gaagcgatgc aggcggttca atttggtaac cccgctgaac
    17581 gcaacgacat tgctgctgga tgttcgccag gaaatgtcga ttatgcatga ggaaaccttt
    17641 ggcccggttc tgccggtggt cgcatttgac acgctggaag atgctatctc aatggctaat
    17701 gacagtgatt acggcctgac ctcatcaatc tatacccaaa atctgaacgt cgcgatgaaa
    17761 gccattaaag ggctgaagtt tggtgaaact tacatcaacc gtgaaaactt cgaagctatg
    17821 caaggcttcc acgccggatg gcgtaaatcc ggtattggcg gcgcagatgg taaacatggc
    17881 ctgcatgaat atctgcagac ccaggtggtt tatttacagt cgtaatgagt aaaagaggcg
    17941 gaggtttttt cctccgcctg tgcgcgtcag agtttagcga atttttcgag ggtgcgaata
    18001 agctgcgtga cgaagccata ttcgttatcg taccaggcga ccgttttcac cagttgtaaa
    18061 tcgcccacgg cggtaatttc cgtttgcgtg gcatcaaaca ccgaaccgaa atggctgcca
    18121 atgatatcgg aagagactat ttcttcatcg gtataaccaa atgactcgtt attggtggtt
    18181 gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt tttttccgag aatggacacc
    18241 agctcagtga ccgaacctgt tttcaccggc acgcgttgcg catgaccttt cagtttgccg
    18301 ctcagttccg ggatcaccag accaatggct tttgccgccc ccgtagtgtg gggaatgata
    18361 ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac gtgggccatc caccagtgac
    18421 tgggtgccag tataggcatg aatggtcgtc atcgtgccga cttctatccc gaaactgtca
    18481 tgcaaggctt tggccatcgg cgcaagacag ttagtggtgc atgacgccac ggaaacaatg
    18541 gtgtcgttgc catccagagt gtcgtcattg acgttataaa cgatagtttt catttcaccg
    18601 gcaggggcgg aaatcaacac cttcttcgca ccagcatcaa gatgcgcctg cgatttctcg
    18661 gcggaggtat aaaagccagt acattcgaca atgatttctg cacctttcgc tttccacgga
    18721 atatttttag cctctttttc ggcgtaaacc gcgatacttt tcccatcaac gataagtgaa
    18781 tcttccgtaa aatcaacgct ccaggggaat ggtccgtagt ttgaatcatg tttcagcagg
    18841 taggcgagaa tttttgggga agtgagatca ttaatagcga caacgtctat gttgcttttg
    18901 acttcaagta atcgacgcaa caccagtcga ccgatacgac caaaaccgtt aataccaact
    18961 ttactcatgg ttttctcctg tcaggaacat tcggatgaaa attgatcctt tccaggctta
    19021 gaccaggatt gtgggatggg caatctccat tcttacagtg aaacgtaacg tactgaaaac
    19081 gggtgaacaa tatttaatga aattttgaga aaagcccgtt atgttaacgg aaaattatgt
    19141 taaagcagga aatgttatgg aaaataaata ctcaaggtta caaatcagca ttcactggct
    19201 ggtctttttg ctggttatcg cagcgtattg cgcaatggag tttcgtggtt tcttcccacg
    19261 tagcgatcgg ccactcatca acatggttca tgtttcctgt ggtatctcaa ttctcgtgct
    19321 gatggtggtt cgtcttttgt taagactgaa atacccaacc ccgccgatta tacctaagcc
    19381 aaaaccgatg atgacgggac tggcgcattt gggacatttg gtgatttatc tcctgtttat
    19441 tgcgctgcca gtgattggtt tggtgatgat gtataaccgg ggcaacccgt ggtttgcgtt
    19501 tggtttgacg atgccttacg cttcagaggc caatttcgaa cgggttgata gcttaaagtc
    19561 gtggcatgag acgctggcga atctgggata ttttgtcatc gggttgcacg ctgcggctgc
    19621 actggcacac cactatttct ggaaggacaa cacacttcta cgcatgatgc cgcgtaaacg
    19681 ttcctgaagg atatttaaag aaaacgcctg tactaaaacc gacccgtggt acaggcgaag
    19741 aatacgggtc tacatcggaa gcgcctatta tatttatttg tatgacaaat aaaaccataa
    19801 tccttgccca tacgtccatc tggcttattt ttaatcaatt cacccgatct ttgatctcat
    19861 caacggtatc aaaataaacc agcgtataac gttcaaatcg aacataagat tcgataaacc
    19921 atggaggtta tatgaaaaaa ctggcactta ttttgtttat gggaacgctt gtttcctttt
    19981 atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca cactgtacgg
    20041 caggcggcaa atttgtctgt aatgatggtt ctattagtgc ctcgaaaaaa acatgcacta
    20101 actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc tagtcagatg cgcggggaaa
    20161 atccccgcgc ttgcccttac ctggacgtgc aggccatgag cgcagcaacc tccttatcac
    20221 cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag gagtgtgaat gtcagaatcg
    20281 taatgcagat aatgagcaga cacaccacca gagggttgtg cttcatagcc tttctccttg
    20341 ccggatggcg ggtaagaggc taagatctga attgctaggt tcattcgttg gcctcggttg
    20401 atagaaatat cggtcggggc cttcgtcttt ctgattcccg gttagcctga aaacagaaag
    20461 tctcaggcac ccgcaggcat cctatgaggt ttccttaggg acgaaaataa tcacttcacg
    20521 aaattgcgtg ctgttttcca gaatttttcg tcattcgggt tagccagttt agccattcgt
    20581 tactctcttc attccaatag cattaatttt ctctgcaata attgttgtaa aaatgtgacg
    20641 caaagaggtt tttggtcata agtaattacc gtcaagtgcc gatgactttc tatcaggagt
    20701 aaacctggac gagagacaac ggtaatgaat acaactccct cacatcgatt aggttttttg
    20761 catcacatca ggctggttcc gttatttgcc tgcattcttg gcggtatctt agttctattc
    20821 gcattaagtt ctgctctggc tggctatttc ctctggcagg ccgatcgcga tcagcgtgat
    20881 gttactgcgg agattgagat tcggaccggg ttagcgaaca gttcagattt tttgcgttca
    20941 gcccggatca atatgattca ggccggggct gcaagtcgta ttgcggaaat ggaagcaatg
    21001 aagcgaaata ttgcgcaagc cgaatcggag attaaacagt cgcagcaagg ttatcgtgct
    21061 tatcagaatc gaccggtgaa aacacctgct gatgaagccc tcgacactga attaaatcaa
    21121 cgctttcagg cttatatcac gggtatgcaa cctatgttga aatatgccaa aaatggcatg
    21181 tttgaagcga ttatcaatca tgaaagtgag cagatccgac cgctggataa tgcttatacc
    21241 gatattttga acaaagccgt taagatacgt agcaccagag ccaaccaact ggcggaactg
    21301 gcccatcagc gcacccgcct gggtgggatg ttcatgattg gcgcgtttgt gcttgccctg
    21361 gtcatgacgc tgataacatt tatggtgcta cgtcggatcg tcattcgtcc actgcaacat
    21421 gccgcacaac ggattgaaaa aatcgccagt ggcgatctga cgatgaatga tgaaccggcg
    21481 ggtcgtaatg aaatcggtcg cttaagtcgt catttacagc aaatgcagca ttcactgggg
    21541 atgacagtag ggactgttcg acagggtgcg gaagagattt atcgtggcac cagcgaaatt
    21601 tcagctggca atgcggacct gtcatctcgc accgaagaac aagcggcggc tatcgaacaa
    21661 actgccgcta gcatggagca actcactgcg acggtgaaac agaatgcgga taacgcgcat
    21721 catgccagca aactggcgca agaggcttct attaaagcca gcgatggcgg gcagacggtt
    21781 tccggtgtag taaaaacgat gggcgctatc tctacaagtt cgaagaaaat ttccgagatc
    21841 accgccgtca tcaacagtat tgctttccag acgaatattc tggcactgaa tgctgccgtt
    21901 gaagccgcgc gagcgggtga gcaaggccgt ggatttgccg ttgtcgccag cgaagtacgg
    21961 acactcgcaa gccgcagcgc ccaagcggcg aaagagattg aaggcttgat cagtgaatca
    22021 gtcaggttaa ttgacctggg gtcggatgag gtggcaacgg cagggaaaac catgagcact
    22081 attgttgatg ccgtcgcgag tgccacacat atcatgcagg aaatcgccgc cgcctcggat
    22141 gaacaaagta gaggcataac gcaggttagc caggcgattt ctgaaatgga taaggtgacg
    22201 caacagaatg cttctctggt agaagaggcc tcagcggcgg cggtgtccct tgaagaacag
    22261 gcggcacgat taactgaggc ggtggatgta ttccgtctgc acaaacattc tgtgtcggca
    22321 gaacctcgcg gagcgggtga accagttagt ttcgctacgg tgtgaaaatg ttcaaggagg
    22381 gatcgacaga tcccttcacc tttcagaacg gcattgattt tcgaatagcg ttaatcatca
    22441 actggcaacc agaagagaac gtcgcatcta cgcgggtcag tattccaatc ggttcgcctg
    22501 caccatgtcc cggaacaggc agggccacca gcgtggcatg acgcaggtcg tcttttacag
    22561 cgccagaagg gacaaaccac acgtaatcgt attcaaccgt aagttgacga gatagcgaag
    22621 cagacagcgt ttcgatacaa cccgaaggaa ttttacagcc ctggctctgc actaatgcat
    22681 ctgaatgctg gcgtggcgca gtgccttctg gtgatacaac gaccggccat tccagcaccc
    22741 ggcttagcgt tacgttctcc tgaagtagcg ggtgattagg gcggacaacc agcttcaacg
    22801 attcaagaaa cagcagttcg taattaagcc cggtcatcag ttcaggatct gacatccgac
    22861 caatgccgat atcgatttcc ccggttttta aacccgccag aatcataggg ttactcattg
    22921 tcgcaacttg caaggtcgtc tctttttgtt gctgatgaaa ctgacctata accgaaggta
    22981 atatccccag tgccgcagta ggtagtgcac caaccctgac gacatcatta ttaagacctt
    23041 ctttacgatg aagcgactgt ccggcagtgt tgatggcgtc aagaactctg actgcatgcg
    23101 ttaaaaattg ttcgccgggt aaggtaagtt gcgccccctg acgaccacgc tcaaacaagc
    23161 gagcgccagt cagcagctcc agttcattca atgtcttaga gagcgcaggt tgactcaaat
    23221 taagggtttc agccgcgcgc cccaaagttc cttgttgtgc gacagctacg aatgtatgaa
    23281 ggtggcgcaa acggatgcgc tgactaaaca gactattttt ttccataagc gatgttaaaa
    23341 acgaagcggt gtcgctgaca agtgaagttg tttgattatg ataacttgat tgcaaaatat
    23401 tattaacaat taaagcaatt gtgttacagc aaaatggata atattgatgt tttcgcggcg
    23461 agatcacagt ttgtaaattc ttcccgccag agtgaatgcg gttacctaca ctccagatta
    23521 ctgaccactg gaggcagaca ctatggcgaa cagcatcacg gcggatgaga ttcgggaaca
    23581 gttttcgcag gcaatgtcag ccatgtacca gcaagaagtt ccgcaatatg gcacgctgct
    23641 ggaactggta gctgatgtga atctggctgt gctggaaaac aatcctcaac tgcacgaaaa
    23701 aatggtaaat gcagacgagc tggcgcgact gaatgttgaa cgtcatgggg cgattcgcgt
    23761 tgggactgca caagagcttg ctactcttcg gcggatgttt gccattatgg ggatgtaccc
    23821 ggtgagctat tacgatctct cgcaggcagg ggtgccggta cattcgacag catttcggcc
    23881 cattgatgat gcttctctgg cgcgtaatcc cttccgcgtt tttacctcct tactccgcct
    23941 tgagcttatc gagaacgaaa ttttgcgcca gaaagcggcg gagattctgc gtcagcgcga
    24001 tatcttcacc ccacgttgtc gacaactgtt agaggaatat gagcagcagg gcggttttaa
    24061 cgaaacacag gcacaggagt ttgtgcagga agccctggaa acgtttcgct ggcaccagtc
    24121 agcaacggta gatgaagaaa cctatcgcgc cttgcacaac gaacatcggt tgattgctga
    24181 tgtggtctgt tttcctggat gccatatcaa ccacctgacg ccacgtacgc tggatattga
    24241 ccgggtgcag tcgatgatgc ctgaatgcgg aattgaaccc aaaattctca tcgaggggcc
    24301 gccgcgccgc gaggtaccga ttttactacg ccagaccagc tttaaagcac tggaagagac
    24361 ggtgttgttt gcggggcaga aacagggcac gcataccgcg cgctttggtg aaattgagca
    24421 gcgtggcgtg gcattaacgc cgaaagggcg acaactgtat gatgatcttc tgcgtaacgc
    24481 tggaaccggg caggataatc tcactcacca aatgcattta caggaaacct tccgcacttt
    24541 tcctgacagt gagtttttaa tgcgtcagca agggctggca tggttccggt accgtctgac
    24601 gccttcaggt gaggcgcatc gtcaggcgat tcatcccgga gacgatccac agcccttaat
    24661 tgaacgtggt tgggtcgcgg cgcaacccat tacctatgaa gatttcttgc ccgttagcgc
    24721 ggcggggatc ttccagtcaa atctgggtaa tgaaacgcag gcacgcaatc acggtaatgc
    24781 cagtcgcgag gcatttgagc aggcgttggg ttgtccggtt ttggatgagt tccagcttta
    24841 tcaggaagcg gaagaacgca gtaaacgtcg ctgtggtttg ctttaaattc tgaccatccg
    24901 cctttgcaaa aatttgcctg attttacaaa cgaatcaggc tcatcccatc gacataaaaa
    24961 aattgccgat ttatgcatat tctctgagtt taacaattgg attattaata aatattgtct
    25021 agagtgagcg gtcataaata agcactttct tgccgctgaa aacgaccagc gcgggaccat
    25081 tcacaacacc agaaggactc actttcaggt atggatcgta gacgatttat taaaggttca
    25141 atggctatgg ccgccgtgtg cggtaccagc ggcattgctt ctcttttttc tcaggcggca
    25201 ttcgcggcag attctgatat tgccgacggg caaacccagc gttttgactt ctccattcta
    25261 cagtcaatgg cgcacgactt agcgcaaaca gcgtggcgtg gtgcgcctcg tccgttacct
    25321 gacacgctgg cgacaatgac gccgcaggct tataacagta ttcaatacga cgccgaaaaa
    25381 tcgctctggc ataacgttga gaaccgtcaa ctggacgctc agttcttcca tatgggaatg
    25441 ggattccgtc gccgcgttcg tatgttttct gtagatccag caacacatct ggcgcgtgaa
    25501 attcactttc gcccggagtt gttcaaatac aacgatgcag gtgttgatac caaacaatta
    25561 gaagggcaaa gcgatctcgg ctttgccggt tttcgcgtgt ttaaagcccc cgaactggcg
    25621 cgccgtgatg tagtatcatt cctcggagcg agttatttcc gcgccgttga tgacacatat
    25681 caatacggtt tgtcggcccg cggcctggcg attgacactt acaccgacag taaagaagag
    25741 ttccccgact ttaccgcctt ctggtttgat acggtaaaac cgggggcaac tacctttacc
    25801 gtttatgcgt tgctcgatag cgccagcatt actggtgcct ataagttcac tatccattgt
    25861 gagaaaagtc aggtgattat ggatgtggaa aatcacctgt atgcgcgcaa agacattaaa
    25921 cagctgggca ttgcgccgat gaccagtatg ttcagctgcg gtactaatga acgtcggatg
    25981 tgcgacacca ttcatccgca aattcatgac tctgatcgtc tgtccatgtg gcggggcaac
    26041 ggcgagtgga tttgccgtcc gctgaataat ccgcaaaaat tgcagttcaa tgcttacacc
    26101 gacaacaacc cgaaagggtt tggtttattg caactggatc gtgatttctc ccattatcag
    26161 gacattatgg gctggtataa caaacgccca agtctgtggg tggaaccgcg taacaagtgg
    26221 ggtaagggca ccatcggcct gatggaaatc ccaacaacgg gcgaaacgct ggataacatt
    26281 gtctgcttct ggcagccaga aaaagctgta aaggcgggtg atgagtttgc attccagtat
    26341 cgtctgtact ggagtgcgca accgcctgtt cattgcccat tagcgcgcgt tatggcgacg
    26401 cgtaccggca tgggcggttt cccggaaggt tgggcaccag gtgaacacta tcccgaaaaa
    26461 tgggcgcgtc gttttgccgt cgatttcgtt ggtggtgatc tgaaagctgc cgcaccaaaa
    26521 ggcattgagc cggtgattac gctttccagt ggggaagcga agcaaatcga aattctctat
    26581 attgaaccca ttgatggtta tcgtattcag tttgactggt atccgacttc ggactccact
    26641 gatccggtcg atatgcggat gtatctgcgt tgtcagggcg acgctatcag tgaaacatgg
    26701 ctgtatcagt atttcccgcc agcgccggat aaacgtcagt atgttgacga ccgcgtgatg
    26761 agttaatcgt tttttcttcg gcaccttctt cgggaggtgc cgtctggtta aacacgatcc
    26821 cgctcgcatt tttccctaag ttaaatgagt aatctgatgg tgtgtatttc agatacacct
    26881 tgtcagccac taacagggag tgcgtatgtt tccagaatat cgagatttaa tatcccgtct
    26941 gaaaaacgaa aatcctcgct ttatgtcctt gttcgataaa cacaataaac ttgatcatga
    27001 aattgccaga aaggaaggtt ccgacggtcg agggtacaat gcggaagtgg tccgcatgaa
    27061 aaaacaaaag ctacagttaa aagatgagat gctcaaaatc ctgcagcagg agagcgtcaa
    27121 agaggtgtaa actttcctaa gccgccagcc aggcggcttt tttaacaact gcatggattg
    27181 actggagata agatgactga aacgataaaa gtaagcgaat cacttgaatt acatgctgtt
    27241 gcagaaaatc acgtcactcc tctttatcag ttaatctgta aaaataaaac ctggttacag
    27301 cagtcgctaa actggccgca gttcgttcag agtgaagagg acacgcgaaa aacggtgcag
    27361 ggtaatgtga tgttgcatca acgcggctat gccaaaatgt tcatgatttt caaagaagat
    27421 gaacttatcg gcgttatctc gtttaatcgt attgaaccac tgaataaaac cgctgaaata
    27481 ggctactggc tggacgaatc tcatcagggg caggggatca tttctcaggc gctgcaggca
    27541 ttgattcatc attacgccca gtctggtgaa cttagacgct tcgtgatcaa atgtcgggtg
    27601 gacaatccgc aaagcaacca ggtcgctttg cgcaatggtt ttatccttga aggttgcctg
    27661 aaacaggctg agttcctgaa tgatgcctat gatgatgtga acttatacgc gcgtattatc
    27721 gattcataat aaccctgcca gcggcgttcg cgtaatgcgc tcctcgccgt taatgacttt
    27781 tggaccacgg acatgaaccg tgtcgccatc aaaggcttca acgacggcgt ggtcggttaa
    27841 ctccacatgg ttttcaatta tcaccgcacc tgtaatacgg ctctcacctt gtattacaac
    27901 gtgctcgtcg agcagaatcg gtccaccacg taccacggca tttccgccaa tcaagacatg
    27961 atgttttaac acacaattac cttccacaat ggcatattcc gccacctgcg aactgtaatg
    28021 aatcgttgga atggcatctt cttctatgcc agctttcacc tgcgcgtgac cgtagacttt
    28081 agcgcaatcg catagccaga cgttgttctc ttcattacct tcaatactgg caaaatcaaa
    28141 aacttcggca cgatgttcaa taaaagcata ccggatgacg gcatcgccat aaatttgtgc
    28201 ctggtggaca atacgcgagg cgctaaccct tgcgcgatca taaatttgta gcaggagttg
    28261 atggtcaggc gttaagcctt gtgctgcgac gatcatagaa tgttgattaa ttaaggcgtg
    28321 accaaatatt ctacattgcc cgcaaaccag tgagtcatga atggtgacac tgtcacttat
    28381 atatgcaccc tggctgattt cactattatc gatccagaca ttatccgttg cgtaaacctc
    28441 tccccacagt acgctggtgc cggtgatgcg cgtattaccg gaaatcaccg ttccgccgaa
    28501 cgcaatagca ttctggtcgt aaatccagca atttccttcc tgtgctaaca ccgtttcgcg
    28561 atcgatccag ccgccagcgg ttcctgctat cacatcgtta aaatcgctaa tggcgataat
    28621 ctgccgtaat aacacatttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct
    28681 tcacttagac gatatttgcg catagcgttt tcccacaggt gacttactat aaccgtagca
    28741 aattctgcgg ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt
    28801 ataaattaaa caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag
    28861 gctactttgg tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc
    28921 aggtttggca ggtcagccac tggttagggg atgggctggt gattctggcg atgatcatct
    28981 ggggattatt aactagcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg
    29041 cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt gtttccggca acgacgatgc
    29101 tggtggcgat tggttttgtt ccgtggtttc gcccactggc ggtgtgcctg ttcagttttg
    29161 gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc gggattatgg cgcggatctc
    29221 accctgaaga agctaccacg cctggactgt atctgccgac agttgccaac aactttatca
    29281 gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag
    29341 gcgttttctc atggctaagc cttgaacctg tgatcttgca gcgtctgcgc agttcgggag
    29401 aattacccac ggcactgcgg acatcactcg gcattcagct cgctcctgcg ctggtggcct
    29461 gtagtgcctg gttgagcgtc aacggcggcg agggtgatac gctggcgaaa atgctttttg
    29521 gttatggact gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc
    29581 catttaatgc ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt
    29641 tgcatctggg gagtggcagc gaaaatggat ttttccatac gctggcggta ccgctgttta
    29701 tctttaccaa ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg
    29761 gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat
    29821 cattcgtgac gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt
    29881 actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg
    29941 tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc
    30001 catgagtatc gccaatgtag agcgcattaa atccattgaa aatctggata atttacacac
    30061 ccgagtcgtt gatctgaata acctcacatt tgatagacag tacgatttta ttctttcgac
    30121 tgtggtactg atgttccttg aggctaaaac catcccaggg ctgattgcca atatgcaacg
    30181 ttgcactaaa cctggtggtt acaacctgat tgtggcggcg atggataccg ctgattatcc
    30241 atgtaccgtc ggcttcccgt ttgccttcaa agagggagaa ttacgtcgat attacgaagg
    30301 ctgggagagg gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg
    30361 taatcgtatt aaactgcgtt tcgccacgat gctggcacgt aaaaaatgac ccgctaagaa
    30421 taaggcgcgt gaaaattccc cacgctgaga tgatttactg ttcttctttt cggtaagcat
    30481 attttttatc gaagggatgt gaaattaatc acagtagtcg aagtttttta gcagcttaac
    30541 ttactgaaat ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt
    30601 ctgaaaaggg gctgaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg
    30661 cgctttttgc tataacttaa aaagtaatat aatcatctca ggaaactatt catgcgtacc
    30721 acatcatttg cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct
    30781 aaaatcaccc agccagataa atattctggt tttttaaaca attactctga tttaaaagaa
    30841 acaacctcag caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc
    30901 aaatatgaca gcatcgtctg gaacccaatc acttattatc cggtgccaaa accttctacc
    30961 caggtagggc agaaagttct ggataaaatt ttgaactata ccaacaccga aatgaaagaa
    31021 gcgatagcgc agcgtaaacc actggttacc accgctgggc cgcgtagtct gattttccgt
    31081 ggggccatta ccggtgtaga taccagcaaa gaagggctgc aattctatga agtggttcct
    31141 gttgcattag tggttgcggg gacgcaaatg gctacaggcc accgtaccat ggatactcgc
    31201 ctctattttg aaggtgagct gattgatgca gcgactaata aaccggttat caaagtcgtt
    31261 cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc caatggcttt cgaaaatatt
    31321 aaacaagtta ttgatgacat ggcgaccgat gccaccatgt ttgacgttaa caaaaagtag
    31381 ttccagacgc gccatcgtta gatggcgctt tttatccggt gtgccgtaaa accccatcct
    31441 tcagggcggg gatataaggt gcggttttcc acctgaccag gtgtttgctg ttgttcaatg
    31501 tattggcgga tgacggatat tggcgcacct ccgcagctac tggcaaaata gccaggactc
    31561 cacaaaacgc ctttgtagta atacctgact gcaatatctg gtcgatctcg tcgcagtaac
    31621 ctacccgata cgcctttgag gctgtttacc agactggata tcgccagttt gggaggttag
    31681 ttaattaaca aatggacgtg atctggttcg ccatccattt caaccagttc agcttcaaaa
    31741 taagcacata catttgaaaa gtaagtgcgt agtttttctg tcgcgtcaaa aaatctggcg
    31801 tcggtatctg gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat gccttccacg
    31861 ccggatatcg gtttctttat tcacagacca aagtatcatt tgacctgtga aacgattaca
    31921 ggcatttaaa ttccagttaa gaccaggtgg tcaacaggag tgtgaaatga ggcg
//
LOCUS       DAZUFB010000050        31453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZUFB010000050 DAZUFB010000000
VERSION     DAZUFB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.050"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1213)
                     /locus_tag="ACHY3M_002573"
     CDS             complement(<1..1213)
                     /locus_tag="ACHY3M_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4317470.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRE"
     gene            complement(1411..1486)
                     /locus_tag="ACHY3M_002574"
     tRNA            complement(1411..1486)
                     /locus_tag="ACHY3M_002574"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1451..1453),aa:Phe,seq:gaa)
     gene            complement(1593..2168)
                     /locus_tag="ACHY3M_002575"
     CDS             complement(1593..2168)
                     /locus_tag="ACHY3M_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4317471.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(2205..3902)
                     /gene="dsbD"
                     /locus_tag="ACHY3M_002576"
     CDS             complement(2205..3902)
                     /gene="dsbD"
                     /locus_tag="ACHY3M_002576"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFS4317472.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(3878..4216)
                     /gene="cutA"
                     /locus_tag="ACHY3M_002577"
     CDS             complement(3878..4216)
                     /gene="cutA"
                     /locus_tag="ACHY3M_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFS4317473.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(4332..5633)
                     /gene="dcuA"
                     /locus_tag="ACHY3M_002578"
     CDS             complement(4332..5633)
                     /gene="dcuA"
                     /locus_tag="ACHY3M_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFS4317474.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(5751..7187)
                     /gene="aspA"
                     /locus_tag="ACHY3M_002579"
     CDS             complement(5751..7187)
                     /gene="aspA"
                     /locus_tag="ACHY3M_002579"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFS4317475.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7524..8000
                     /locus_tag="ACHY3M_002580"
     CDS             7524..8000
                     /locus_tag="ACHY3M_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFS4317476.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(8016..9272)
                     /gene="yjeH"
                     /locus_tag="ACHY3M_002581"
     CDS             complement(8016..9272)
                     /gene="yjeH"
                     /locus_tag="ACHY3M_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFS4317477.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            9548..9841
                     /locus_tag="ACHY3M_002582"
     CDS             9548..9841
                     /locus_tag="ACHY3M_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4317478.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            9885..11531
                     /gene="groL"
                     /locus_tag="ACHY3M_002583"
     CDS             9885..11531
                     /gene="groL"
                     /locus_tag="ACHY3M_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4317479.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            11669..12022
                     /locus_tag="ACHY3M_002584"
     CDS             11669..12022
                     /locus_tag="ACHY3M_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFS4317480.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12226..13095)
                     /locus_tag="ACHY3M_002585"
     CDS             complement(12226..13095)
                     /locus_tag="ACHY3M_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFS4317481.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(13485..14513)
                     /gene="epmB"
                     /locus_tag="ACHY3M_002586"
     CDS             complement(13485..14513)
                     /gene="epmB"
                     /locus_tag="ACHY3M_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS4317482.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            14555..15121
                     /gene="efp"
                     /locus_tag="ACHY3M_002587"
     CDS             14555..15121
                     /gene="efp"
                     /locus_tag="ACHY3M_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4317483.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15173..15298
                     /gene="ecnA"
                     /locus_tag="ACHY3M_002588"
     CDS             15173..15298
                     /gene="ecnA"
                     /locus_tag="ACHY3M_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS4317484.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            15409..15555
                     /gene="ecnB"
                     /locus_tag="ACHY3M_002589"
     CDS             15409..15555
                     /gene="ecnB"
                     /locus_tag="ACHY3M_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS4317485.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            15737..16054
                     /gene="sugE"
                     /locus_tag="ACHY3M_002590"
     CDS             15737..16054
                     /gene="sugE"
                     /locus_tag="ACHY3M_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS4317486.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(16051..16584)
                     /gene="blc"
                     /locus_tag="ACHY3M_002591"
     CDS             complement(16051..16584)
                     /gene="blc"
                     /locus_tag="ACHY3M_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS4317487.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(16673..17806)
                     /locus_tag="ACHY3M_002592"
     CDS             complement(16673..17806)
                     /locus_tag="ACHY3M_002592"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS4317488.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(17869..18228)
                     /gene="frdD"
                     /locus_tag="ACHY3M_002593"
     CDS             complement(17869..18228)
                     /gene="frdD"
                     /locus_tag="ACHY3M_002593"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS4317489.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(18239..18634)
                     /gene="frdC"
                     /locus_tag="ACHY3M_002594"
     CDS             complement(18239..18634)
                     /gene="frdC"
                     /locus_tag="ACHY3M_002594"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS4317490.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(18645..19379)
                     /gene="frdB"
                     /locus_tag="ACHY3M_002595"
     CDS             complement(18645..19379)
                     /gene="frdB"
                     /locus_tag="ACHY3M_002595"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS4317491.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(19372..21180)
                     /gene="frdA"
                     /locus_tag="ACHY3M_002596"
     CDS             complement(19372..21180)
                     /gene="frdA"
                     /locus_tag="ACHY3M_002596"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS4317492.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            21505..22482
                     /gene="epmA"
                     /locus_tag="ACHY3M_002597"
     CDS             21505..22482
                     /gene="epmA"
                     /locus_tag="ACHY3M_002597"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS4317493.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            22701..24203
                     /gene="yjeM"
                     /locus_tag="ACHY3M_002598"
     CDS             22701..24203
                     /gene="yjeM"
                     /locus_tag="ACHY3M_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFS4317494.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            24255..24569
                     /gene="yjeN"
                     /locus_tag="ACHY3M_002599"
     CDS             24255..24569
                     /gene="yjeN"
                     /locus_tag="ACHY3M_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFS4317495.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            24566..24880
                     /locus_tag="ACHY3M_002600"
     CDS             24566..24880
                     /locus_tag="ACHY3M_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFS4317496.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24909..28232)
                     /gene="mscM"
                     /locus_tag="ACHY3M_002601"
     CDS             complement(24909..28232)
                     /gene="mscM"
                     /locus_tag="ACHY3M_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFS4317497.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(28254..29222)
                     /gene="asd"
                     /locus_tag="ACHY3M_002602"
     CDS             complement(28254..29222)
                     /gene="asd"
                     /locus_tag="ACHY3M_002602"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFS4317498.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(29319..30371)
                     /gene="rsgA"
                     /locus_tag="ACHY3M_002603"
     CDS             complement(29319..30371)
                     /gene="rsgA"
                     /locus_tag="ACHY3M_002603"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFS4317499.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            30466..31011
                     /gene="orn"
                     /locus_tag="ACHY3M_002604"
     CDS             30466..31011
                     /gene="orn"
                     /locus_tag="ACHY3M_002604"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFS4317500.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            31222..31297
                     /locus_tag="ACHY3M_002605"
     tRNA            31222..31297
                     /locus_tag="ACHY3M_002605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31255..31257,aa:Gly,seq:gcc)
BASE COUNT         7550 a         7930 c         8225 g         7748 t
ORIGIN      
        1 tttctctgtt tgcatccaga aaatcggccc accactgtag catcagtcgc cgttcttcca
       61 gatgttctgc tttatgaatg taagcagcac gtacaccgtt acgttcctga tggctcatct
      121 gacgttcaac tgcatcgcgg gaccacaaac ctgattctat taacgcacta catgccatcg
      181 cccggaatcc atgaccacag acatcctggg ttgtgtcata ccccataacc ctcagggctt
      241 tatttacggt gttttcactg attggtttac gtgggtcatg tgctcccgta aagatcagac
      301 cattttcacc agcccatgtc tgaagttctg ccagtatggc tacaacctgt ttgctgagtg
      361 gcacaagatg tggtgttcgc atcttggatc cccggtgtga gaattttacg cctggcagag
      421 gttcgcgttc ggctggaata gtccacatgg cattgtcaat atcaatttca gaccagcggg
      481 cgtatctgag ttcactggaa cgaataaaaa ccagtaaatt cagcataacg gcaagctggg
      541 tgacaggttg ccccttgtaa ctgccaatac gcgacaaaag ttcaggcagg cgagaaagat
      601 caagagcggg gcgatgctgg cgttttaccg tagtcaatgc gccagccata tccagagcag
      661 gattatatct gatgatgcca gactgtacgg cataacgcat tacggcagat attctttgct
      721 gcagacgact ggcgatttca taaatttgtt tagcttctgc agcacgaaca gggataagca
      781 ggtccggggt attgagtgtt gtgatatccc gattgccgat aaaggggaat acgtgtgtct
      841 cgaggctttt gagtacttta tcgctgtgtg attgtgccca ttgtttatta ctggcatgcc
      901 agcgtcgtgc aatatgttcg aaagagagtt gtaaatcagg gactactttt ttagctcttt
      961 tctgttcgca agggtcaacc ccctccgaaa tcagttttcg ggcctcatcc cgttttgtac
     1021 gagcttcgga gagagatact tctggataca caccaagtgc cagagtcttc tctttaccca
     1081 gaatacgata acggagacgc cagtaacggg aaccattagg atgtaccagc aggaacatgc
     1141 cagccccgtc agtgagttta taagccttgt cagtgggctt tgcagcccgg atttttgcgt
     1201 cagtcagtgc catagttcag ccttctgaat gcgttaaaga gggtacaaat tctgtcgaac
     1261 tggggtatgc ccgcaattat acccacacaa tgaggttgat gtggggagaa tttggttgag
     1321 ttcggtagaa ttgatttgga ggaagaacac ttaaatcatg gtgttctgaa acgaaaaacg
     1381 gacctccgtg gaggtccgtt gatatgaatt tggtgcccgg actcggaatc gaaccaagga
     1441 cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta
     1501 aaccgtaatc tgcacatctc gtcaacctaa tttcagtaaa agcgattcaa ctgcttaaga
     1561 ttgcggcaaa tcgcttcttt tctgtgtttc tgtcaggtca atgcaccacc ctggcggcag
     1621 cgggcgaagc gcaggatatc ttccgccagc cgatgtgcag tgtcgacatc cgcctgacta
     1681 cgattcacca gcattcggct taaacagcct tccagcacca gttccatctg ctttgctacc
     1741 atcgccggat cgtcaacttc cagcgtggtt aacagttcgt gggtgaaatc gtaggccgcg
     1801 cttttttgct gatcggccag ttgatgaata gggtggccag gatcgggata aaacgtacag
     1861 gcagcgataa atagacagcc cggatagcgg ttgtttttaa cgcactccga taacgcctga
     1921 taacgtgcca gcagcttttg ttcggcggtt tgcgtttcgt ccagcatcag ctgacgacgc
     1981 cagacatcta tctgttggct aagataacgc agcgcatcgt agaggattgc ctctttgtct
     2041 ggccagaagc ggcgtagctc gtccagtgga taatccacac gttcagcaac catctccagc
     2101 gtggtgttgg caatcccttg taattctaat aatttcaggg cttctcccag tacatcttca
     2161 cgttgcacgc tattttcctc cgtctttccc actgcaagtg tcgttcacgg ttggcgatcg
     2221 cgcaaatgtg cgctgaaggt ttcagcatcc ataaagcccg tgacgcgtgc ttgtggatgc
     2281 tcctggcctt gtccgtcaaa aaagagaatt gtcggtaggc caaggacatt aagatgcttt
     2341 aacagcgcca catcttgtgc gtcgttggcc gtgacgttgg cctgaagtaa gaccgtgtct
     2401 gctaacgctt tttgcacctg cgggtcgctg aaggtgtatt tctcaaactc tttacaggcg
     2461 acgcaccagt cggcataaag atctaacatc accggtttgc ctttgacttc aacgagcgcc
     2521 tgatttaact catctaccgt tttgatttgt gtaaagttga gatgcgtctg agtttgcgcg
     2581 gtatgcgtcg caccaaatgc ccaatcctga agtgggcgca cgctaaccaa tgccgctgcc
     2641 agcaggatta tttgcaccac gcgcatccag ccgcgtttag cctgtaggct ggtgataaag
     2701 gcccagccaa agaatgcgac acccagcgcc gaccacaagc gtaatcccca tacatcacca
     2761 atcactcgct ccagcaggaa gaccggcagt gcgaggatca caaaaccaaa cgcggttttg
     2821 acttgttcca tccacgggcc gcttttcggc agcaagcggt taccaaagac ggtaattagc
     2881 atcagcggca ggcccatgcc caacgcatag agataaagcg tgccgccgcc cagccacatg
     2941 ttcccgcttt gggcgatata cagcagaatc gcgctaagcg gtgcggtggt gcatggtgaa
     3001 cagatcagtc cggcaatcgc ccccataaca aacacaccgc caggtgagcc gccctgttgg
     3061 cgattgctca tcaacgtgag acgtgtttgc agcgaagagg ggagttgtag agtaaacaag
     3121 ccaaacattg acatcgccag caaggtaaag acgatggcga ggccaatgag cacgtatggg
     3181 tgctgtagcg ccgcctggaa ctgtaaccct gcggcggcaa ctaccagacc cagcgccgtg
     3241 taagtcagtg ccatcccctg cacataaata aaggtcagca acaatgctct ggcagtggag
     3301 agccgctgtt taccacccag cacgatgcca gaaatcagtg ggtacattgg cagcacgcat
     3361 ggcgtaaagg cgataccaat accgatcaac aacgcccaga gcgcggaaaa gggcaattgc
     3421 gcggtgtgct gctcttgctg cggaacagac acaggctgtg acgctgcgtt gttggctacc
     3481 acttcgctta acggaacggt tttggtttct ggcggataac agaaaccggc atcagcacag
     3541 ccttggtagg tgacagttaa cgtcgctccc gcactcgcct ggttgatggt tacgggaagc
     3601 gtcagccgat cgcggtaaat ctcgcttttg ccgtaaaact catcttcatg ccagacgcct
     3661 tgcggcagct gcacgtcggc aattttcgcg tgttccggcg taatgcggat ctgtttacgg
     3721 tagaggtagt aaccgtcttt gatctgccag gtcagattca ggtcatgttg gttttgctga
     3781 aaatcaaaag caaaggcttg atccgcgggg acaaaatgtg aacgtcccgg cgcgtcgaat
     3841 aatccggcaa aaacggaagt gctgcaaagt agcaggatca gcgtaaagat gcgttgagcc
     3901 atgagaggta atctgtgtct ccgtgtgtaa caggtaaaac cagaagttcc ggggtttgat
     3961 atggatgatg agacttcagg cattccagca gtgcctgctg gtgagatacg gtagttttta
     4021 aaatcatctg cacttcgtat tcttgctcca gcttaccttc ccagtaatag agagaggtag
     4081 cgccggggat caaggtcgcg caggccgcca gtttttccgc cagcactttg gcggctaaat
     4141 cctgggctgt cgcttcatcc ggtgcggtac atagcaccac gacagacgcg gtattcgaac
     4201 ttttttcatc aagcataaac acctcgcaag aacagatgga accgcaagag agaggtcact
     4261 atacaacgga cgggggaagg atgttagtcg gcgggaaaga aagcggggcg tgaacgcccc
     4321 gcgattggtc attacagcat gaagctaccc agcacgaagc cgaagcaaac ggccagggca
     4381 acacccagag tacccgggat gaagaacgga tggttgaaga cgaatttacc gatacgggtt
     4441 gtacccgtgt catccatctg taccgcagca accagcgtcg ggtaggtcgg cagaatgaac
     4501 agaccagaca ccgcagcgaa agaagcaaca gcggtcagcg gtgaaacgtt cagtgccagg
     4561 gccatcggca tcagtgcttt tgcggttgca gcctgagagt acagcagagc agaagcaaag
     4621 aagaagatga cggccagcag ccacggatga ccctgaatca cttcaccagc ggtatctttg
     4681 atccagtcga tgttgttgga aacgaaagta tcgcccagcc acgcaacacc caggatacaa
     4741 atacaggcgc tcatacctgc tttgaaggtg ctggagttga ggatgttgtc ggtatcgact
     4801 ttacagataa cggtggtcag agttgcaacg ctgagcatga tgatcaggat tgcgttggtg
     4861 gtgttcatca gcggtttttc aaccagaccc atgcttgggc tgttgatgat tgcatagata
     4921 accacgccaa ctacgcccag caggaacagc cagacggacg tttttgcacc ggatttgatt
     4981 tcaatctgct tttcaccgcg cagttcaacc aggccctctt ccagacgctt gcgataaatc
     5041 ggatcgtcag agagtttgga gttgaacagc atagtgacca gaaaggacat caccagaacc
     5101 gccagcaggg tggacgggat gaccacggag agcagatgga ggtagctgat gccatgacct
     5161 tccatcacgg aagacatgta aaccactgcc gctgagattg gcgatgcggt gatcgcaatc
     5221 tgcgcggata ccactgcagt agacagcgga cggcaaggtt tgacgccttg ttccttcgca
     5281 acttcagcga taactggcag tgtcgccaga gatatgttgc cagtaccagc aaagatagtc
     5341 aggaaatagg tcacgatcgg tgcgaggatc gtgatgtatt tcgggttacg gcgcagcagc
     5401 ttttctgtct gatgaaccag atagtccaga ccgccagcaa cctgcatggc agaaatagcg
     5461 gcgataaccg ccatgataat ggaaatgaca tcgaacggga tgttaccggg tttaacgcca
     5521 atagcggcaa gaaccagcac ccccaatccg cctgcaaaac caatacctat tccccccaat
     5581 ctggcgccca agaagatcgc cagcaaaact atgatgagtt ctacaactag catattagcc
     5641 ttccttgttt tttaacaagt tgatattagg ttgttatttt taagttactg ctcacaagaa
     5701 aaaaggcacg tcatctgacg tgccttcttt atttgtacta ccctgtacga ttactgttcg
     5761 ctttcatcag tatagcgttt tgctttgtaa gccgggtgca tcagattctg tacggagaaa
     5821 atatcgtcaa gttccgcttc agtcaacaga ccgcgttcca gaacgacttc acgtacactc
     5881 ttaccggttt cggcacagat tttacccacg atgtcaccgt tgtggtgacc gatgaacggg
     5941 ttcaggtaag taacgatacc gatagagttg taaacgtaac cttcgcacac ttctttgtta
     6001 gcagtgatgc cgttaatgca tttttccagc aggttgtagc aagcgttggt cagaatgtga
     6061 acggattcga acatggcctg gccaatgacc ggctccataa cgttcaactg cagctgacct
     6121 gcttctgctg ccatggtaac agtggtgtcg ttaccgatga ctttgaagca tacctggtta
     6181 accacttccg gaacaaccgg gtttacttta gctggcatga tggaagagcc cgcctgcagt
     6241 tccggcaggt tgatctcgtt caggccggca cgtggaccag aagagagcaa gcgcaggtcg
     6301 ttacagattt tggacatctt cacagccagg cgtttcagcg cgccgtgaac cataacataa
     6361 gcgccgcagt cagaggtcgc ttcgatcagg tcttcagccg gtacgcatgg gaagccagtg
     6421 acttcagcca gttttttcac tgccagcgga gagtactctt tcggcgtgtt cagaccagta
     6481 ccgattgctg ttgcgccaag gttaacttcc agcagcagtt cagcggtacg ttggatattt
     6541 ttcacttctt ctttcagcag gatgctgaaa gcgcggaatt cctgaccgag ggtcatcggt
     6601 actgcgtcct gcagctgggt acgacccatt ttcaggatgt cctggaattc gacagcttta
     6661 cgttcaaagc cttcacgcag ttggttaatc gcatctacca gcttaatcag ggaagagtaa
     6721 actgcgatac ggaaaccggt cgggtaggcg tcgttagtgg actgacattt gttaacatgg
     6781 tcgttcgggt tcaggtactg atattcacct ttctggtgac ccatcagttc cagaccgata
     6841 ttggccagca cttcgttggt gttcatgttt acggaagtac ctgcgccgcc ctggtagacg
     6901 tctaccggga actgatccat gcattttccg ttgttcagga cttcatcaca tgcggcaatg
     6961 atggcattcg ctacactttt aggaatggtt tgcagctctt tgtttgccat agctgcggct
     7021 tttttaacca ttaccatacc gcgaacaaat tcaggaatat cactgatttt gttgttgctg
     7081 atatagaagt tttcaatcgc tctcagagtg tgaacaccat agtaggcatc agctggaact
     7141 tccctggtac ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg aaccttcttt
     7201 ttcaagctgc caatgatttg ctttaaacac acagaatata tgtggtttcg aatgtttttc
     7261 gaccgacgat tatcccctgc atcgaccgaa tatccgagat catatgctgc ttgaggattt
     7321 ctaccgtaat ctggatcact ttaagtgtcg gtttttaccc cttaattatt aatttgtgaa
     7381 atagatcacc gctttgggat tactaccaaa aatagttgcg caaacatctt gaaattttgc
     7441 taatgaccac aatataagct gaacgcgatt cgcaacccat tcaggtagcc gggttttacc
     7501 ggctgctatt acaggagaaa cctttgcgct ggttaccttt tattgccatt ttcctttatg
     7561 tctatattga gatttcaatc tttattcagg ttgcccatgt attgggggta ttgctgaccc
     7621 tcgtgctggt tatattcacg tcagttatcg ggatgtcact ggtacgtaac cagggcttta
     7681 agaatttcgt gctgatgcag caaaaaatgg cggcgggtga aaacccagcg gcggagatga
     7741 ttaaaagtgt ttcgctgatc attgccggtt tgctgctttt attaccgggc tttttcaccg
     7801 acttcctcgg tcttctgctt ttattaccgc cggtgcaaaa gcatctgaca gtgaagttga
     7861 tgccgcattt gcgcttttct cgtatgccag gcggcggttt tagcgccggg accggtggcg
     7921 gtaatacttt tgatggtgag taccagcgaa aggatgatga gcgcgaccgc cttgatcata
     7981 aagacgatcg ccaggattaa tgtcgaaacg ccggattatg tggttatgcc attttccggc
     8041 gtttttcgtt ttggcagcaa cagccataac cccgccagca tgatcagcgc atagagactt
     8101 ttccagccga ccattgccag taacagaacg cataacagcc cgccaaccac cgccagtagt
     8161 cgataacgtc cttgcaataa tttacagcct gccagcatgc ataacagata aatcataata
     8221 aagatgccat tggcataaat aataagagcg tccagattga tctctaaagc atgaatcacc
     8281 aacgtgctca ccacacagca gccgagcacc gcattgaggg cattattcgg gatatggcga
     8341 gaagagaggc gtgccaggta gtggtcagga ttatgttgcg cctgcgacca gaccaggcgg
     8401 gcgaagctct gtatataaat gttgagactg gcaaagcagg ccagatagcc aatcacgcag
     8461 gcaatccata acgctcctac accgaacaac tgcactacaa tttttggaag cgatgctgcc
     8521 gccgccattt tttcaccata ggcgtcgaag tgtaagacga ctaccgtaca gccccagtag
     8581 actaatcctg ccagcagcag accaatcatc aatgcgcgag gaaaatcacg ctctggattt
     8641 ttaaattccg aggcaagatg ggcaaatgct tccagaccga caaaacacca gaacatcact
     8701 gataacgcag caaataaccc ggtaagttcg atattaccag gtgccgggaa ggggatattc
     8761 gcaggtttga tatcgcccgc ccaccagata gcgacaatca gcgcgacgat aagtccggca
     8821 ataacggttt gtagattagc actggaactg gcaccgcgag taccgatata ccacaccagc
     8881 gccagcgtac cgagttctgc caacaacagt tgccagctat gccagccaaa cattgcctgg
     8941 ccgaacccgg cggcaatttg tagcgcggca ggcaaaccca cgggaatgac cgataaaaac
     9001 aaccagccgg tgactcgctc aagtcgcgaa ccaaacgcca taccgacgaa gtgcgcgaca
     9061 ccgcctgcgc tgggatagtg gcgacccaga atcgcaaaca caatcgcaat cgggaacact
     9121 aagataatca aaacgggcca cgcccacagg ctgttattgc ccgctaccag cgcagctaac
     9181 gcaggaacgg caaacacgcc agtgcctaat aatgacgtcg atagcaggcc aatgccttgg
     9241 gccagcccca gttcttgttt gagtccactc atgggttgat atccgattgc gcccaaattt
     9301 tgggcaactg cgtagatttt cgatggtagc acaatcagat tcgcttatga cggcgatgaa
     9361 gaaattgcga tgaaatgtga ggtgaatcag ggttttcacc cgattttgtg ctgatcagaa
     9421 ttttttttct ttttccccct tgaaggggcg aatcctcatc cccatttctc tggtcaccag
     9481 ccgggaaacc acgtaagctc cggcgtcacc cataacagat acggactttc tcaaaggaga
     9541 gttatcaatg aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta aagaagttga
     9601 aactaaatct gctggcggca tcgttctgac cggctctgca gcggctaaat ccacccgcgg
     9661 cgaagtgctg gctgtcggca atggccgtat ccttgaaaat ggcgaagtga agccgctgga
     9721 tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat ctgagaagat
     9781 cgacaatgaa gaagtgttga tcatgtccga aagcgacatt ctggcaattg ttgaagcgta
     9841 atcctcgcac gacactgaac atacgaattt aaggaataaa gataatggca gctaaagacg
     9901 taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta ctggcagatg
     9961 cagtgaaagt taccctcggt ccgaaaggcc gtaacgtagt tctggataaa tctttcggtg
    10021 caccgaccat caccaaagat ggtgtttccg ttgctcgtga aatcgagctg gaagacaagt
    10081 tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc taaagcgaac gacgctgcag
    10141 gcgacggtac caccactgca actgtactgg ctcaggctat catcactgag ggtctgaaag
    10201 ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa gccgttaccg
    10261 ctgcagttga agaactgaaa gcgctgtccg taccgtgctc tgactctaaa gcgattgctc
    10321 aggttggtac catctccgct aactccgacg aaaccgtagg taaactgatc gctgaagcga
    10381 tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt ctgcaggacg
    10441 aactggacgt ggttgaaggt atgcagttcg accgtggcta cctgtctcct tacttcatca
    10501 acaagccgga aactggcgca gtagaactgg aaagcccgtt catcctgctg gctgacaaga
    10561 aaatctctaa catccgcgaa atgctgccgg ttctggaagc tgttgccaaa gcaggcaaac
    10621 cgctgctgat catcgctgaa gatgttgaag gcgaagcgct ggcaactctg gttgttaaca
    10681 ccatgcgtgg catcgtgaaa gttgctgcgg ttaaagctcc gggcttcggc gatcgtcgta
    10741 aagctatgct gcaggatatc gcaaccctga ctggcggtac cgtaatctct gaagagatcg
    10801 gtatggagct ggaaaaagca accctggaag acctgggtca ggctaaacgt gttgtgatca
    10861 acaaagacac caccactatc atcgatggcg tgggtgaaga agctgcaatc cagggccgtg
    10921 ttgctcagat ccgtcagcag attgaagaag caacttctga ctacgaccgt gaaaaactgc
    10981 aggaacgcgt agcgaaactg gcaggcggcg ttgcagttat caaagtaggt gctgctaccg
    11041 aagttgaaat gaaagagaaa aaagcacgcg ttgaagacgc cctgcacgcg acccgtgctg
    11101 cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta gcgtctaaac
    11161 tggctgacct gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt gcactgcgtg
    11221 caatggaagc tccgctgcgt cagatcgtcc tgaactgcgg cgaagaaccg tctgttgttg
    11281 ctaacaccgt taaaggcggc gacggcaact acggttacaa cgcagcaacc gaagaatacg
    11341 gcaacatgat cgacatgggt atcctggacc caaccaaagt aacccgttct gctctgcagt
    11401 acgcggcttc tgtggctggc ctgatgatca ccaccgagtg catggttacc gacctgccga
    11461 aaaatgatgc agctgactta ggcgctgctg gcggtatggg cggcatgggt ggcatgggcg
    11521 gcatgatgta attgccctgc acctcgcaga aataaacaaa cccccgggca gaaatgtctg
    11581 ggggtttttc ttttggtcat ctttctagta taagattcag acacggacga cgcgagtggc
    11641 gtccagctca ttgattatgg ggaataacat gcacgtaaaa tacttagcag ggattgtcgg
    11701 tgccgcgcta ctgatggcgg gttgtagctc cagcaacgaa ttgagtgctg ccggtcagag
    11761 tgtacgcatt gtggacgagc agccaggcgc agagtgccag ctgattggta ctgcgacagg
    11821 taagcaaagc aactggcttt ccgggcaaca cggagaagag ggcggttcta tgcgcggcgc
    11881 agcaaacgat ctgcgcaacc aggcggctgc aatgggcggt aacgtgattt atggcatcag
    11941 cagcccgtcg cagggaatgt tgtccagttt tgtcccgacg gatagccaga ttatcggtca
    12001 ggtttataag tgcccgaact gatgtgatcc agaggctggg tgacaaacgt atcattgcct
    12061 gatgcgcttc gcttatcagt atctacagca ggcattgcaa ctgattgaat gcttgagaat
    12121 ttgtaggccg gataaggcgt ttacgccgca tccggcatga ccaacgatca cgttatcagt
    12181 catttcaggc tggatggctc cagcctcgca ttagcaaaga tgagattatt tcgcctgtgg
    12241 tgcagttttg ctggtggatt gaacacgtaa gtgatgcagc gcgcgtaagc attgatcctg
    12301 ggttagcggt tgggcgttat tcgccgctac ggtgcgaaca tagacctgac atggcacacc
    12361 cgcgagtttt ttcagtcgtt ggctgaaatc aagcattcgg cgagtgatcg cctctatttc
    12421 tttacccggc gttgcttcac gcgttttcac cactgcgccg ctaaattgtt ctttgccact
    12481 ttcatcttta aagcggtaaa gcaccgccag ataatggtcg aacaggttat gtttccattc
    12541 cggttgggaa taggtgtcat attccagatt gccattttcc tggcagtcaa cgtcatataa
    12601 cggcaggaaa tctaacagtg aggtaatacg taatgccagt tcacgcattt ccgcattatt
    12661 aattgcatga atgattgctc gcgccagcat ttcaatctgc aactcattga cgactaactc
    12721 acatttgcta ccgtcatgta gcgttaatac gaaggtaaga ttttcgcctt gattatcggt
    12781 aagttccagc gtgttaaccc gacgattaat atcggcattt ttaagctcat caaccagaat
    12841 ttctgggatg ttttctgcca ttttcttaat gactttatcg cgagcttgct catactgaag
    12901 gcgtgcagca gcatccagtt tatgcttttg atgcatacga ctttccagcg caatcagcag
    12961 gtcgcgcagt tccatgacag acataaagaa caacgattct ttattgcgag gttctttaat
    13021 tttcagcgcc agtgcaatga aattattact cttgcgaata acaccggtat taacaccctt
    13081 gatgcttatg gccatgtagg ttctccctaa ccatttctca attaaataat taattttaat
    13141 ttataagcca gataaatggg cttggtagta atagttgtta aaataacata aatagccgta
    13201 ctcactctat ataaacagaa aagaaaggtt aattattggt gttggctata taaagagtaa
    13261 caacagcaat gtatatgaat aattaaaggt tatgcaacgg gcaaagatta aatttcccag
    13321 tttaatgaca ggttgctgta cttgcgtttg ttgctcttgc cggatgcggc gtaaacgcct
    13381 tatccggcct acagttcatt gcaaatccat tcaatttcaa tgcacaatgt aggcctgata
    13441 agcgtagcgc atcaggctaa tgtttaactt ccctgtttaa tcagttactg ctggcgtagc
    13501 tggagatcca gcggcgtttt gctgggttcg ccgccgattt ctcgcgccag tttcggcacc
    13561 agataacccg acaccagtgt cagcaactca cgcataatct gccgtgcttc gtcatcactc
    13621 accataaaat gcgccgcgcc ctgtacttta tcgagcacat gcaggtaata cggcattacg
    13681 ccggcatcga acaacgcatt actcaggttt gccagcgttt gtgcgttatc gttcacacca
    13741 cgtaacagaa cgctctggtt aagcagggtg acaccgacac ggcgcaattt agccatcgcc
    13801 tgacggaacg tttcatctac ctcattggca tgattgatgt gattcaccag caagatttgc
    13861 agcgtagaac gggcaaagcg ttcaaccagc gcctcggtga tacgtgccgg gatcacaatc
    13921 ggcagacggc tgtgaatccg cagacgtttg atatgcggga tggcttccag ttgtgtgagc
    13981 aaccagtcca gctcgtgatc tttcgccatc agcggatcgc cgccggagaa aatcatctcg
    14041 tccagttccg gatgcgcagc aacatactca agagcagtct gccagttacg cttgttgccc
    14101 tgattttcgg cataggggaa gtggcgacgg aagcaatagc ggcaatttac cgcgcagccg
    14161 cctttgacca gcaaaagcgc ccggttgtgg tatttatgca acaaaccagg cactacgctg
    14221 tgctgttctt ccagcgggtc ggtggagaat ccggacgcga cgacaaactc atcttgcgag
    14281 gtaagtacct gacgcaaaag aggatcgtcc ggattgcctt tctccatgcg atcgataaat
    14341 gagcggggca cacgcagggc aaacagcttt ttggcgctgc gtccggccaa cagtttttcg
    14401 tccgcgtcta tattcaaaag acgcagaagt tcatcaggat cggtcacaac atcggcaagt
    14461 tgcgttaacc aatcttctct ggatggggta tttagggtta caatatgcgc cattttgtgg
    14521 cttagctacc aattaacaaa tttcagaggg ccttatggca acgtactata gcaacgattt
    14581 tcgtgctggt cttaaaatca tgttagacgg cgaaccttac gcggttgaag cgagtgaatt
    14641 cgtaaaaccg ggtaaaggcc aggcatttgc tcgcgttaaa ctgcgtcgtc tgctgaccgg
    14701 tactcgcgta gaaaaaacct tcaaatctac tgattccgct gaaggcgctg atgttgtcga
    14761 tatgaacctg acttacctgt acaacgacgg tgagttctgg cacttcatga acaacgaaac
    14821 tttcgagcag ctgtctgctg atgcaaaagc aattggcgac aacgctaaat ggctgctgga
    14881 tcaggcagag tgtatcgtaa ctctgtggaa tggtcagccg atctccgtta ctccgccgaa
    14941 cttcgttgaa ctggaaatcg ttgataccga tccgggcctg aaaggtgata ccgcaggtac
    15001 tggcggcaaa ccggctaccc tgtctactgg cgctgtggtt aaggttccgc tgttcgtaca
    15061 aatcggcgaa gtcatcaaag tggatacccg ctctggtgaa tacgtctctc gcgtgaagta
    15121 atgcggttgt ggtgcggcct gcaggctgca ccatcactta ttcaggtcag agatgatgaa
    15181 acgccttatc gttcttgttt tgcttgccag cacgctgctc acgggctgta acaccgctcg
    15241 cggtttcggc gaagacatca aacatctcgg caactccatc tctcgcgctg ccagctaatt
    15301 tttcttctct tccgaaaaat catcagattt ccgtcatttt tggcgatgtt gtctattatt
    15361 aatttgctat aggcaaacat aaataacatt acctaaaagg aagacgttat ggtgaagaag
    15421 acaattgcag cgatcttttc tgttctggtg ctttcaacag tattaactgc ctgcaacacc
    15481 acgcgtggcg ttggtgaaga catttctgat ggcggtaacg cgatttctgg cgcagcaacg
    15541 aaagcgcagc aatagcaata agcaataacg gtacgacagg tatgtcgtgc cgtttgtttt
    15601 ttctgcgata gtcactaagg taatagttga aattcccctg ccacctggca aaatatccgt
    15661 tcaaccatca gctttgcagg acgacctgca aacgcctctt ttcaccgggg acggccccaa
    15721 ttctccggag cctgatatgt cctggattat cttagttatt gctggtctgc tggaagtggt
    15781 atgggccgtt ggcctgaaat atacccacgg ctttagtcgt ttgacgccga gtgttattac
    15841 cgtgacggcg atgattgtca gtatggcgct acttgcctgg gcgatgaaat cgttaccagt
    15901 agggacggct tatgccgtgt ggacgggtat tggcgcagtc ggcgcggcca tcaccggcat
    15961 tgtgctgctc ggtgagtccg ctaacccgat gcgcctggcg agtctggcgt taatcgtatt
    16021 ggggattatt ggtctgaaac tcagcactca ctaactaccg ggctgctgta cccaaataaa
    16081 cttactgaca tcaaaccctt cccgggtcgc gactgccagc atctcctgtt tcacttcgtc
    16141 agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc cgcaaaccag
    16201 cgcatggcgg tattcccgat cgagtgcaat aacgttataa ccgccataga aaggaccaaa
    16261 gaatgacact ttcagcgcag cgcgagttgg tgcgccggta aagtacgctt tcccttcact
    16321 ctgctgccac attcctctgt cagggttata gcctttatta atgacattca ggccgccgtc
    16381 atcacgcagg ctgtatgttg cggtgacttt ttccagtcca cgttcaaaac ggtgatcaaa
    16441 acgggcaatc tcataccagg taccgagata acgtttggcg tcgaaattat tcactactgt
    16501 tacgccacgc ggcggcgtag gagaactgca ggcaacgacc agaaatgcag ctgtcgctgc
    16561 ggcaacgaga gggagcaggc gcataaatgt ttccttactg gtttttttct aagtgtagat
    16621 gacagcaagg aaaagcgcag aaaaggtccg aaaattcgga cccgatgaaa ttttactgta
    16681 gagcgttgag aatctgccag gcggcggcga ctctcgctgg attgggatag tttttgttag
    16741 ccagcatcac tatacccagc tctttttctg gaataaacgc gacatagcta ccaaatccgc
    16801 cggttgcccc tgttttatgt acccatgatg cgcgtactgc aggagttggg ggcgtaatcg
    16861 cttttacggg gtgtgctgcc agtgcaattt tattgccact gccgttaatg atgctgtcag
    16921 gatttaccgg ccagtccagc atttcccagc ccaggccctg atacatatcg ccggtttgcc
    16981 agtagcgaga ttgtgccagt tgtatccctt gctgaagtgt tttgtcgttg atatcacggg
    17041 gattcatatt gcttcgtacc cagcaggcca tatcttcaat ggtcgacttc acaccataag
    17101 tttcagcatc taacgcccct ggcgaaacat gaactgcctt gccttcgcga tatccccagg
    17161 cgtaattctt ttcttctgcg ggcggtacat taatccacgt atggttgagt ttgagtggct
    17221 ggaagacacg tgtttgcatc gcctgctcaa aactcaaacc agacggcttc acagccagtg
    17281 cgccgaacaa accgatactg gagttggcat acagacgttg tgttcctggc gcccatgcag
    17341 gctgccagtt ttgatagaag cgcagcaagt cgcttgagga tttcacctca tccggcacct
    17401 gcaatggcag gccgccagca gtgtaggttg cgagatgtaa tagtgtgatc ccattccact
    17461 gtttagcggt aagttcaggc cagtattttg ttgtgggatc gcttaacttg atttcccctc
    17521 gagcaatagc gtcgccacca agcacgcccg taaatgtttt gctgaccgaa cctaactcaa
    17581 acaacgtttg ctgtgtgacg ggctgctttt tggcgatgtc cgcatagccc caggtaaagt
    17641 aataaggttt accctgataa attaccgcca ccgccatacc ggggatcttt tgttgctcta
    17701 taagcggggt aattgtgcga tgcacaatat cgttgatttg ttgaggggca gcaaatgtgg
    17761 agcaagaggc ggtaattaat aaggcgcaga gcgtcgtttt gaacataagg tctggtttcc
    17821 atacaaaacg gcccgccata ggcgggccgg atttacattg gcgatacgtt agattgtaac
    17881 gataccaatc agcgtgacaa ctgtcaggat agcagccaga ccgtagaaaa cccatttgcc
    17941 cgcaggcacg tgaattttca gatcgtgcat cgcgtggtgc atacggtgta aaccacacca
    18001 cagcggcaga acgatcatca ggaacaggaa tacgcgacca atgaagctct gcgcgaacgc
    18061 cagaacgcgc tcgtagctca gcgcatcacc cggaaacagc cccagtggca gcagaatacc
    18121 caccagcagg atcatcaccg gcgcaataat tgcgctccac ataccaccgg ccccgaagag
    18181 gccccagaat accggttcgt cagaacgctt tggatttgga ttaatcatct caggctcctt
    18241 accagtacag ggcaacaaac aggattacga tggtggcaac cacagttacc gcccagagac
    18301 ttttgataat tggctctggt cccatttttt cgtcttttac aatgatattg gccgctttcg
    18361 gtgccagttc aaaccaggtt ttggtgtgca gcagagctgc cgccagagtg atcaggttaa
    18421 tgatcacgat aaccgggttt tgtaaaaagt cgacgaatcc cgcccaggct tccgggccat
    18481 ttttcagggc aaacagcccg aaaatcagtt caatgctgaa ccacacagcc ggaaccgccg
    18541 tgccttcgcg cagcatgtaa aagcgataaa acggcaattt tttccaccag gtggacgtca
    18601 ttggccgtac atacggttta cgtttagtcg tcatgttgca ctccttagcg tggtttcagg
    18661 gtcgcgataa gaaagtcttt cgaactttct actttgccct gctgaatggc cgcagccgga
    18721 tcgacgtgtt tcgggcagac ttcggagcaa tagcccacga aagtacagct ccatacgccg
    18781 ttctggctgt tcaactgcgc catacgctcc ttcttaccgt ggtcgcggct atcttcgtta
    18841 taacgatgcg ccagcgtaat ggcagccgga ccgatgaact ctgggttcag gccaaactgc
    18901 gggcacgcgg cgtagcacag accacagttg atgcaaccgg agaactggtg atactttgcc
    18961 atctgcgccg gggtctggat gttagtaccc tgatccgcgg tgcgggagtt gccgatgatg
    19021 tacggtttga tcgcttccag actttcgatg aagtgggtca tatcgaccac cagatcgcgt
    19081 tcaatcggga agttagctaa cgcttcaacc ttcataccgt cggtgtaatc acgcaggaag
    19141 gttttacatg ccagttttgg cacgttgtta accatcatgc cgcaggaacc acaaatcgcc
    19201 atacggcagg accagcggta actcaggtcc ggtgccaggt tgtctttgat gtagcccagc
    19261 gcatccagta atgaggtagt tgcgtcataa ggcacttcat agaatgcgct atgcggtgcg
    19321 gtatcgactt ccgggttata gcgcaccacc tcaattttca ggtttttcat ctcagccatt
    19381 cgccttctcc ttcttattgg ctgcttccgc cttatcggct gcatccgctt cgccaccgta
    19441 aacgcgttta gctggcggca gcgtagtaat cttcacgtcg ctgtactcca ggcgagtcgt
    19501 gccatcagca tcgcggaagg cgagggtgtg tttgaggaag ttgacgtcgt cacgctcggt
    19561 gcaaccttcg tccagacgct ggtgtgcgcc gcgggactct ttacgtgcca ttgcggagtg
    19621 cgccatacat tcagcaacgt tcagaccgtg gcccagttca atggtgtaga gcaggtcggt
    19681 gttgaacacg ctggaggtgt cggtgatgcg cacgcgcttg aagcgttcct gcagctctgc
    19741 cagcttgtcg atggttttct gcatcagttc cggcgtacgg tagataccgc aaccttcttc
    19801 cattgccagg cccatttcgt cgcggatctt cgcccagttt tcgccgccat cctggttaac
    19861 cagatctttc agacgttgtt caacgccagc tgcctgcgct tcaattgccg cttcgttgcc
    19921 attaccggca gttgctgcac gctctgtcgc ttgttcaccg gccagacggc cgaagaccac
    19981 cagttccgcc agggagttag aacccagacg gtttgcaccg tgcagaccaa cagaggaaca
    20041 ttcacccacg gcgaacagac ctttaatgcg ggtttcacag ttctgatcgg tttcgatacc
    20101 gcccatggtg tagtgtgcgg tcggacgtac cggaatcggt tctttaaccg gatcgacgcc
    20161 aacgtacgct ttcgccagtt cgcagatgaa cggcagacgt tcatgcagtt ttttctcgcc
    20221 gaggtgacgc aggtcgagat aaaccacatc gccacgcggc gtggagatgg tgttgccttt
    20281 acgccattcg tgccagaagg cctgagaaac tttgtcgcgt ggacccagtt ccatatattt
    20341 gtttttcggc tcgcccagcg gagtttccgg gcccatgccg taatcttgca gataacggta
    20401 gccatttttg ttgaccagaa taccgccttc accgcggcag ccttcggtca tcaggatacc
    20461 ggaacctggc agaccggttg ggtgatactg aacgaattcc atgtcacgca gcggaacgcc
    20521 gtggcttagc gccataccca taccgtcacc ggtaacgatg ccgccgttgg tgttgtaacg
    20581 ataaacgcga cccgcaccgc cagtagccat aacgaccgcg ttagcacgga tctgcaccag
    20641 cgtgccttcc atcatgttca ttgctaccac gccgcgaaca tgaccatcat caaccagaat
    20701 atccagcacg aaatgttcgt caaaacgctg gatctgcggg aattgcagag aggtctggaa
    20761 cagcgtgtgc agcatatgga agccggtctt atcggcggcg aaccaggtgc gctcgatttt
    20821 catgccgccg aagcgacgta cgttgacgct accatccggg cgacggctcc atgggcaccc
    20881 ccacagttcc agttgggtca tttcggttgg gcagtggtgg acgaaataat ccacgacatc
    20941 ctgctcacac aaccagtcgc cacccgctac tgtatcgtga aagtgatatt cgaagctgtc
    21001 atgatcctgc gcgacagcgg cggagccccc ttctgcagca acggtatggc tacgcatcgg
    21061 gtatactttt gagattagtg cgatttttgc atttggattt gcctgcgcgg cagcaattgc
    21121 agcacgtaat cccgcgccac cggcgcctac aatggcaaga tcggcttgaa aggtttgcac
    21181 gacattcctc cagattgttt ttatcccaca gccccgtact tcagggtaag tgcctgaaag
    21241 ttacggtctg cgaacgctat tccactgctc ctttataggt acaacagtat agtctgatgg
    21301 ataagtctga aatttgacga gatcgatttt tttagtgcgc aagagggcta aattatcact
    21361 gaagatgatt aatttaatta ctaaaccatc agatcgtgct ttttttagtc actgcccacc
    21421 gctgtttgat tcctgcgtaa aatttgtctc gccgtcgcgt tgcgagtaga cttcgtgccc
    21481 ttgtcaaaaa ctggagattt aactatgagc gaaacggcat cctggcagcc gagcgcatcc
    21541 attcctaact tattaaaacg cgcggcgatt atggcggaga tccgtcgttt ctttgccgat
    21601 cgtggagtgc tggaggtgga gacgccttgt atgagccagg cgacggtaac cgatattcat
    21661 ttggtcccgt ttgagacacg tttcgttggc cccgggcatt cgcaggggat gaatctctgg
    21721 ttaatgacca gcccggaata ccatatgaaa cgcctgctgg ttgccggttg tgggccggta
    21781 ttccagctgt gccgcagctt ccgtaatgaa gagatggggc gttatcacaa ccctgagttc
    21841 actatgctgg agtggtatcg accgcactat gatatgtacc ggttgatgaa cgaggtggac
    21901 gatctcttac aacaggtgct ggactgcccg gcagcagaaa gcctttctta tcaacaagct
    21961 ttcttgcgtt atctggaaat tgacccgctc tctgccgaca aaacgcaact gcgggaagtc
    22021 gcagcgaaac tggatttgag caatgttgct gataccgaag aagaccgcga cacgctgcta
    22081 caattgctgt ttacctttgg cgtagagcca aatattggca aagaaaaacc gacctttgtg
    22141 taccactttc cagccagcca ggcatcactg gcgcaaatca gtaccgaaga tcatcgggtc
    22201 gctgaacgct ttgaggttta ttataaaggt attgagctgg cgaatggttt ccatgaattg
    22261 acggatgccc gtgagcagca acaacgcttt gaacaagata accgtaagcg cgcggcgcgc
    22321 ggtttgccgc agcaccccat tgaccagaat ctgattgaag ccttgaaagt cggtatgcct
    22381 gactgttccg gcgtggcatt aggtgttgat cgtctggtga tgttggcgct gggcgcggag
    22441 acactggctg aagtcatcgc ctttagcgtt gaccgggcat aattctgaaa aattactgaa
    22501 ttaacagcga agaatggcgt gataagcgcc attctgtagc atatgttttc atttttccgt
    22561 taaaggccac aatttaagac tggatatttg atatcatcca ggtatcaatt ctgtattggt
    22621 ttttacgctg cctgctcaat gttgcgcagt gcagttcagt gggcacgtgt tatacacgcg
    22681 ctgaaatgaa ggatggtttc atgcctcaca cgataaaaaa gatgagtctg ataggactca
    22741 tattgatgat ctttacttcc gtatttggat ttgccaatag cccatcggct tattacttaa
    22801 tgggttatag tgcgattccc ttttatatat tttctgcatt gttattcttt attccattcg
    22861 ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggcggt atctattcct
    22921 ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg tggttttcct
    22981 cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc tcaacattcc
    23041 tctatggtag cgacatgacc cagcactggc gtattgctgg actggagcct acgcaggtgg
    23101 ttggtctgct ggcagtggca tggatgattc tggtcaccgt cgttgcttca aaggggatta
    23161 ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg aatttagtat
    23221 tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg caggatatta
    23281 atttccttgc atcaccgaac ccaggttatc agtccggtct ggcaatgcta tcgtttgtgg
    23341 tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc gataaaacgg
    23401 aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt atttcaatcg
    23461 gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag gtattaagta
    23521 atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc ggggtgacgc
    23581 tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc
    23641 gtattaccgg actttcgatg ttcctcgctt ataccggtgc gttctttacg ctttgctatt
    23701 caccgctgaa agccatcatc caggggacgc cgaaagcgtt gtggccggaa ccgatgacgc
    23761 gcctgaatgc gatggggatg ccgtctatcg ccatgtggat gcagtgcggg ttggttactg
    23821 tcttcatcct gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt aacaagctga
    23881 cgctgatggc gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg gctttcccat
    23941 tttttaaagc acgtcaggat ctcgacagac catttgtgat tttcaaaacg catatgtcgg
    24001 caatgattgc aacagtggtt gtcgtactgg tggtgacatt tgcgaacgtc ttcaccatca
    24061 ttcaacctgt ggttgaagcc ggagactggg acagcacatt gtggatgatt ggcggccctg
    24121 tcttcttctc gctgttagcg atggcgattt accagaacta ttgcagcaga gtggcaaaaa
    24181 atccgcagtg ggcggtggaa taacccgatg ccctatcgtc tacctgacga tagggcgtaa
    24241 agacaggaaa tgttatggat gacaccagcc gggacccggc aataaccgaa gatgaaatca
    24301 gggcgctgca attttctgcc ggggatgtag cggaaataga gcagacgatt ctctcttttg
    24361 tcgatgcctg ccacacccgt aaagtggcga tggtggtggg tagcaccatt aacaccctca
    24421 aagatcgcga tggtaaacgg tggggaaacc tgccggatat ttattgcgct tatcttatcc
    24481 gatgtctggt ttttcgcggg gaattagtgg gatacggcga tttgtttcgt atgcgctata
    24541 gcgaaattaa gcgaccggtt accttatgag tgcgcgcatg tttgtcttat gctgcatctg
    24601 gtttattgtg gcgttcctct ggataaccat cacttccgcg ctggacaaag agtggatgat
    24661 tgatggacgc gggattaaca acgtctgcga tgtgttgatg tatctcgaag aagacgatac
    24721 ccgtgatgtt ggtgtgataa tgacgttacc gctctttttc ccttttctct ggttcgcttt
    24781 gtggcgaaaa aaacgcggct ggtttatgta cgccaccgcg ctggctattt tcggctactg
    24841 gctgtggcag ttttttctgc gctatcagtt ttgtttgtga gccggattgg ttcatccggc
    24901 acacaaactt acaaacttcc cgcctgacga cctttgcccg cagacgtcag cgttctcccc
    24961 gtttgtttac cgttgaggct ttccagacgc atctggaagg gcgggaatgg catatcgata
    25021 ccgtgggcgt ggaagcctgc cagaatcagc tggtggatct catggcgtag cggcatacgg
    25081 tgacccatct cagcggcgta aatacgcagc tcgaaaatct gaatcccctg ttgcagatcc
    25141 accaggaaga cttccggtgc cgggttgtcg atcaccagcg agcagcgacg cgctgcggtg
    25201 agcaggattt ccgtcacttc ttcgctattg gcatcggcag gggccggtat cgtcaacacc
    25261 acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat aaacgccttg
    25321 ttcggcacga ttatctcttt acggtcccag tcgctgatgg tggtggcgcg ggtgttaatt
    25381 ttcgtcacgc taccggtaag atcgcggatc gttaccgtat cgccaatgcg aatcggtttt
    25441 tcgaacagga taatcaggcc agagataaag ttggcgaaaa tctcctgcaa accaaaaccg
    25501 agaccaacac cgagcgcggc aaccagccac tgcaatttcg accactcaat accaatcatc
    25561 gagaagccga ccagcccgcc aatcagcatc agcagatatt tggtgatggt ggtgatggcg
    25621 tagcccgtac ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag cagcgcgggc
    25681 aggttgcgca ccagctgcgt ggtgatgata aacaccagaa tggcaatcag caccgcaccg
    25741 agggtaattg gctccagact ttctacgccc tgtaccgtgg aggtgacatc ccacagcgaa
    25801 atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat gacagaaagc
    25861 agggcgatca acatcaaaat tgagcgcacc agccgcaagg attgcgcact gatggcatcg
    25921 agatcgactt cgctttcatc gacttcaatt gccccttccg ggctactgtg atgatgcgct
    25981 tcctcttcgc cacgcgcgcg ttgcgctaac atctctgccc ggcgatgctt cgcccggtca
    26041 aacgccagcc tgcgccgctg gatcagcatc cagcggcgga taacgtgata aaccaccagt
    26101 agcaggaacc agatggcaac cgaggtttca agcctcgcta acagtgcctg cgccgttgcc
    26161 agataaccca ccgctgacgc cagaatggca accagtggtg cgccaatcat catgttccac
    26221 agcatatggt tggtaatgtt gtcgccgctg ccctctttgt tgaggtacag cgggatcccg
    26281 gcctttttca ggctgagggt gaccaccgcc agcgcaccgc agatgaggat aaagcaaagc
    26341 cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat cgaacatcat cagcgccata
    26401 atcagcggca caataagccc gatgctcatc aggtagtagc gcatcccacg ggaaacacgt
    26461 tcgcgcggcc agccaaaatg agcgataaac aagccgttcg ggcgggcaaa ggtggcgcaa
    26521 atcatcacta cccacagcag cggcacggtg gccgttacgc catcgccaat cgcgaccgcc
    26581 agcggataag gccacgcctc gcgcaagccg taacccagcg tcatccacag caccggcagc
    26641 ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg tcagccagaa gtgatcctga
    26701 gtcactttgc cgactttcgc cgccgaacgt tcaagaaaac gggtgaaata gcggcgcgag
    26761 taaatactgc aaccgaccag aatcaacgcg ccaaacagcg gcaaaatcgt ctctttgctg
    26821 gtcagcatca tcacactggc tttgcccaac tgactgaagg tgtccagcga aatgagacga
    26881 cgcagatcct gggcgatttc cagtggccag gcgatggtca tcgggcgcac gtcagaggtc
    26941 cagaacagat agcgatgcgt cgcttcattc acctctttca gcgcatcctc aagttgcccg
    27001 ttggagactt tcagcttggt cagttccagc agtagcgtgt cgccaccctg caacaatgag
    27061 ttcagcaact cacgctgagt gcgtagctgt gcttccagaa tacggttttg ctcggcggtc
    27121 agcggctgac cgtcggcctg atgaatttgc cgtagcagcg gctgtttatt aagcagatcc
    27181 tcataacgta accgttgcac acgcaactgc gccatttcgg tatcaagctg ttgtggtttc
    27241 ggcatttccg gcagccgtgc cacctgcgcc cgcaacgctt cgccgagcag gttggacgat
    27301 cccagccatt gcgactgttc acgcagcgta ttcagcgcct gccgaacctg taacgtctgg
    27361 ctggtggcct ggcgctgttg cgaggcaacg agatccatcc gctgcgcctg ttgattcagt
    27421 gccgccgata gttcgcggtt aattttgaat tgcgcgacga tgtctttcgg caaatcggcg
    27481 ctgttttctg ccagcaattc ggtactttcc agcgcccgct ccgcctcaag ctgacgttgg
    27541 ctgttcaact gattacgtaa ggcctgcaaa tacgcatcca gttgctggct ctctttttcc
    27601 gccagctctg agcgtaagcg cgctaattcc tgacggttat tggcagacag ctgcgccagt
    27661 tccagttcat caacgagcgc tttcaggcgt gcggagtcag actgcaacgc gaaattttgt
    27721 gcctgattga gcggggtatt gccagtaagc gttcccaggc ggcgctcgat ctcatttaac
    27781 tgacggcggg cgtcggtttg ctgttgcggc agttgattca gcgaatcggc aatctcgcgg
    27841 gcgcgctcct gctcttgctg ggcctgacgg cttttatcca gcaactggct gctgacctgg
    27901 agaatttcct gattcagcgc gtcggtagac attcccggcg acacgctgcg cggctcgtca
    27961 cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt tcggataatt atcgataact
    28021 tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt cctcaagcgc atttaaggca
    28081 gactggagcg cctctacgac ttccggctgt gcgggtttcg ccgcttttgc ctgctccagt
    28141 tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt acgcccccca actgaggcac
    28201 caggccatca gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat ggattagacc
    28261 tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt ctgcttcgat ctcttcagca
    28321 ggaagtgggg cgggttcagc gtctggcgta acaaaggttt cggtagatac tgccagcggc
    28381 tggccaattt tcgtgacaga caggctttcc agttgctcaa ccagattcac tttacccggt
    28441 gcaaacaggt tgataacggt agagccgagt ttaaagcggc ccatttcctg gcctttcagc
    28501 agtgccacag aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat gataccttcg
    28561 cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc tgcctacaat cgtcgccccg
    28621 accagaatct gcgccatcgg gccaaattcg gtatcgaaca ggcaaatcac gcgttcgtta
    28681 cgggcaaaca gattcggcac gttctgagcg gtgagatggt taacggagaa gagatcgccc
    28741 ggcacgtaga tcatctcacg cagaataccg ttgcacggca tgtgtacgcg atggtagtca
    28801 cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga acaggtccgc catcagatag
    28861 ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt tggcttgcag gattttatct
    28921 tcttcgattt tacccaactg gctgataacg ccatcggcag gcatgaccag gacattcgga
    28981 tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga caaagaattc gttaaaggtg
    29041 cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt cgaccttgta gtatttaacg
    29101 aacagatcga taaccagttt tgtcagccat cctgcccgct tgcttgcgcc ccaacccgcc
    29161 aggcgagtaa gccatagttt cggcagaatg tactgtagcg aaagtttaaa tgaatttaac
    29221 aaggtagcct ccaggccatt gttttgtcgt tcctgatccg gccaacatgc cggatcctga
    29281 aaaaaagggg acgattctaa cgacggttag cttaattgtc agtcatccgt atcagaaaag
    29341 tttttacgcg tttttacctg cgccatgctt tccagaatac ggtgatagtt ttcgaaacgg
    29401 gtttccgcga ttttcccttc ctcaaccgct tcccggatag cgcagcccgg atcggtatcg
    29461 tgtttgcaat cgcgatattt acacagacct aaatagtcat ggaattcgac aaagccctga
    29521 gtgatttgtt ccggctccag gtgccagagg ccgaactcac gcacaccagg ggaatcaatc
    29581 acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg tcgtggtgtg ctgaccgagc
    29641 cccgagttgt cagagacatc gttggtcagg atctcttttt gcagccccag caacgcattc
    29701 agcaggctgg atttgccgac gccagactgc ccggcaaaaa tgctgatgcg cccggtcaac
    29761 gcctcttcca gcggttttag cccatcctga gtatggctgg aaaccatcaa tacgcgataa
    29821 ccgatattgc ggtagatatc catctgctcg ttgacgaacg ccatgccttc gtcgtccagc
    29881 aggtctatct tgttgagcac aataatcggc tcaatctgca aggtttcgca ggccaccagg
    29941 taacggtcga taatattgag cgacagctcc ggcaaaatgg cggagacaat gacaatctgg
    30001 tcgatgttgg cggcaatagg ttttacgccg tcgtagaaat ccggacgcgt caacaccgag
    30061 gtacgctcat gcaccgcttc cacgatccct ttgacattta cgccttccgc cgccggttta
    30121 cccggacgcc agactacgcg gtcgccggtt accagcgaac ggatggtacg gcgaatattg
    30181 cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt gcataccaaa gcggctgatg
    30241 acgatacctt catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt ctccttagac
    30301 gttttaagac gacgctggtg attggcgttc acgcggcgct gctggccttt ggagagttta
    30361 tttttactca atcgtacaga ctcctggtcg cccctgatgg gcaaaacatc tatgatacac
    30421 gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa atagcatgag tgccaatgaa
    30481 aacaacctga tttggatcga tcttgagatg accggtctgg atcccgagcg cgatcgcatt
    30541 attgagattg ccacgctggt gaccgatgcc aacctgaata ttctggcaga agggccgacc
    30601 attgcagtac accagtctga tgaacagctg gcgctgatgg atgactggaa cgtgcgcacc
    30661 cataccgcca gcgggctggt agagcgcgtg aaagcgagca cgatgggcga tcgggaagct
    30721 gaactggcaa cgctcgaatt tttaaaacag tgggtgcctg cgggaaaatc gccgatttgc
    30781 ggtaacagca tcggtcagga ccgtcgtttc ctgtttaaat acatgccgga gctggaagcc
    30841 tacttccact accgttatct cgatgtcagc accctgaaag agctggcgcg ccgctggaag
    30901 ccggaaattc tggatggttt taccaagcag gggacgcatc aggcgatgga tgatatccgt
    30961 gaatcggtgg cggagctggc ttactaccgc gagcatttta tcaagctgta aaatttttcc
    31021 tggtcacgta agcgccggaa tccacttgcg gcggcgtgat tttgacgcta aattgaacgt
    31081 cttgctgata atttcagcac ttgagataaa aacgcaaaaa aaactttttt ggggggttgc
    31141 agagggaaag atttcttgta taatgcgcct cccgtaacga cgcagaaatg cgaaaattac
    31201 gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg tagagcacga ccttgccaag
    31261 gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa tttgaaaagt gctgcaaagc
    31321 acagaccatc caatcgcagt atttcgaatt gcgacaaggc ggcaactgaa cgaatccttg
    31381 ggaacttaca aaagtatgtg actaaggtga gtgaaggcag ccaaggcagt agcggttcga
    31441 aagacgaaga tta
//
LOCUS       DAZUFB010000051        30837 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZUFB010000051 DAZUFB010000000
VERSION     DAZUFB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.051"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..73
                     /locus_tag="ACHY3M_002606"
     CDS             <1..73
                     /locus_tag="ACHY3M_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317501.1"
                     /translation="GLRFAIREGGRTVGAGVVAKVLS"
     gene            303..686
                     /gene="secE"
                     /locus_tag="ACHY3M_002607"
     CDS             303..686
                     /gene="secE"
                     /locus_tag="ACHY3M_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4317502.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            688..1233
                     /gene="nusG"
                     /locus_tag="ACHY3M_002608"
     CDS             688..1233
                     /gene="nusG"
                     /locus_tag="ACHY3M_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4317503.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1392..1820
                     /gene="rplK"
                     /locus_tag="ACHY3M_002609"
     CDS             1392..1820
                     /gene="rplK"
                     /locus_tag="ACHY3M_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4317504.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1824..2528
                     /gene="rplA"
                     /locus_tag="ACHY3M_002610"
     CDS             1824..2528
                     /gene="rplA"
                     /locus_tag="ACHY3M_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4317505.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2941..3438
                     /gene="rplJ"
                     /locus_tag="ACHY3M_002611"
     CDS             2941..3438
                     /gene="rplJ"
                     /locus_tag="ACHY3M_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4317506.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3505..3870
                     /gene="rplL"
                     /locus_tag="ACHY3M_002612"
     CDS             3505..3870
                     /gene="rplL"
                     /locus_tag="ACHY3M_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4317507.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4190..8218
                     /gene="rpoB"
                     /locus_tag="ACHY3M_002613"
     CDS             4190..8218
                     /gene="rpoB"
                     /locus_tag="ACHY3M_002613"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4317508.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8295..12518
                     /gene="rpoC"
                     /locus_tag="ACHY3M_002614"
     CDS             8295..12518
                     /gene="rpoC"
                     /locus_tag="ACHY3M_002614"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4317509.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVIVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12731..13268
                     /gene="yjaZ"
                     /locus_tag="ACHY3M_002615"
                     /pseudo
     CDS             12731..13268
                     /gene="yjaZ"
                     /locus_tag="ACHY3M_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(13678..14811)
                     /gene="thiH"
                     /locus_tag="ACHY3M_002616"
     CDS             complement(13678..14811)
                     /gene="thiH"
                     /locus_tag="ACHY3M_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFS4317510.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14808..15578)
                     /gene="thiG"
                     /locus_tag="ACHY3M_002617"
     CDS             complement(14808..15578)
                     /gene="thiG"
                     /locus_tag="ACHY3M_002617"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFS4317511.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15580..15780)
                     /gene="thiS"
                     /locus_tag="ACHY3M_002618"
     CDS             complement(15580..15780)
                     /gene="thiS"
                     /locus_tag="ACHY3M_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFS4317512.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15764..16519)
                     /gene="thiF"
                     /locus_tag="ACHY3M_002619"
     CDS             complement(15764..16519)
                     /gene="thiF"
                     /locus_tag="ACHY3M_002619"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFS4317513.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16512..17147)
                     /gene="thiE"
                     /locus_tag="ACHY3M_002620"
     CDS             complement(16512..17147)
                     /gene="thiE"
                     /locus_tag="ACHY3M_002620"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFS4317514.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17147..19042)
                     /gene="thiC"
                     /locus_tag="ACHY3M_002621"
     CDS             complement(17147..19042)
                     /gene="thiC"
                     /locus_tag="ACHY3M_002621"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFS4317515.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19123..19223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19275..19751)
                     /gene="rsd"
                     /locus_tag="ACHY3M_002622"
     CDS             complement(19275..19751)
                     /gene="rsd"
                     /locus_tag="ACHY3M_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFS4317516.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19846..20619
                     /gene="nudC"
                     /locus_tag="ACHY3M_002623"
     CDS             19846..20619
                     /gene="nudC"
                     /locus_tag="ACHY3M_002623"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFS4317517.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20659..21723
                     /gene="hemE"
                     /locus_tag="ACHY3M_002624"
     CDS             20659..21723
                     /gene="hemE"
                     /locus_tag="ACHY3M_002624"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFS4317518.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVSLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21733..22404
                     /gene="nfi"
                     /locus_tag="ACHY3M_002625"
     CDS             21733..22404
                     /gene="nfi"
                     /locus_tag="ACHY3M_002625"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFS4317519.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22447..23037
                     /gene="yjaG"
                     /locus_tag="ACHY3M_002626"
     CDS             22447..23037
                     /gene="yjaG"
                     /locus_tag="ACHY3M_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFS4317520.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23224..23496
                     /gene="hupA"
                     /locus_tag="ACHY3M_002627"
     CDS             23224..23496
                     /gene="hupA"
                     /locus_tag="ACHY3M_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFS4317521.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23509..24204
                     /gene="yjaH"
                     /locus_tag="ACHY3M_002628"
     CDS             23509..24204
                     /gene="yjaH"
                     /locus_tag="ACHY3M_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFS4317522.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24206..24631)
                     /gene="zraP"
                     /locus_tag="ACHY3M_002629"
     CDS             complement(24206..24631)
                     /gene="zraP"
                     /locus_tag="ACHY3M_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFS4317523.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(24634..24762)
                     /locus_tag="ACHY3M_002630"
     CDS             complement(24634..24762)
                     /locus_tag="ACHY3M_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317524.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            24869..26245
                     /gene="zraS"
                     /locus_tag="ACHY3M_002631"
     CDS             24869..26245
                     /gene="zraS"
                     /locus_tag="ACHY3M_002631"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFS4317525.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26242..27567
                     /gene="zraR"
                     /locus_tag="ACHY3M_002632"
     CDS             26242..27567
                     /gene="zraR"
                     /locus_tag="ACHY3M_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFS4317526.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27564..28853)
                     /gene="purD"
                     /locus_tag="ACHY3M_002633"
     CDS             complement(27564..28853)
                     /gene="purD"
                     /locus_tag="ACHY3M_002633"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4317527.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKVFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28865..30454)
                     /gene="purH"
                     /locus_tag="ACHY3M_002634"
     CDS             complement(28865..30454)
                     /gene="purH"
                     /locus_tag="ACHY3M_002634"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4317528.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7262 a         7995 c         8443 g         7137 t
ORIGIN      
        1 cggtctgcgt ttcgcaatcc gtgaaggcgg ccgtaccgtt ggcgcgggcg ttgtagcaaa
       61 agttctgagc taattgccga taacatttga cgcaatgcgc actaaaaggg catcatttga
      121 tgcccttttt gcacgctttc gtaccagaac ctggctcatc agtgattttc tttgtcataa
      181 tcattgctga gacaggctct gttgagggcg tataatccga aaagctaata cgcgtttcga
      241 tttggtttgc ctcgcgatcg cggggtgaaa atgtttgtag aaaacttctg acaggttggt
      301 ttatgagtgc gaataccgaa gctcaaggaa gcgggcgcgg cctggaagcg atgaagtggg
      361 tcgttgtggt ggcattgctc ctggtggcga ttgtcggcaa ctacctttat cgcgacatta
      421 tgctgccgct gcgtgcgctg gccgtagtaa ttctgattgc tgcagcgggt ggtgtcgcgc
      481 tgttaacgac aaaaggtaaa gctaccgttg cttttgcccg tgaagcgcgt accgaagtcc
      541 gtaaggtcat ttggccgact cgccaggaaa cattgcacac cacgctgatt gtggctgcgg
      601 ttaccgcagt aatgtcactg atcctgtggg gactggatgg tattctggtt cgcctggtat
      661 cctttatcac tggcctgagg ttctgagatg tctgaagctc ctaaaaagcg ctggtacgtc
      721 gttcaggcgt tttccggttt tgaaggccgc gtagcaacgt cgctgcgtga gcatatcaaa
      781 ttacacaaca tggaagattt gtttggtgaa gtcatggtac caaccgaaga agtggttgaa
      841 atccgtggcg gtcagcgtcg caaaagcgaa cgtaaattct tccctggcta cgtcctcgtt
      901 cagatggtga tgaacgacgc gagctggcac ctggtgcgca gcgtaccgcg tgtgatgggc
      961 ttcatcggcg gtacttccga tcgtcctgcg ccaatcagcg ataaagaagt cgatgcgatt
     1021 atgaaccgcc tgcagcaggt tggtgataag ccgcgtccga aaacgctgtt tgaaccgggt
     1081 gaaatggtcc gtgttaatga tggtccgttc gctgacttca acggtgttgt tgaagaagtg
     1141 gattacgaga aatctcgtct gaaagtgtct gtttctatct tcggtcgtgc gaccccggta
     1201 gagctggact tcagccaggt tgaaaaagcc taacccagcg atcaaaaaag cggcgattta
     1261 atcgttgcac aaggcgtgag attggaatac aatttcgcgc cttttgtttt tatgggcctt
     1321 gcccgtaaaa cgatttttta tatcacgggg agcctctcag aggcgttatt acccaacttg
     1381 aggaatttat aatggctaag aaagtacaag cctatgtcaa gctgcaggtt gcagctggta
     1441 tggctaaccc gagtccgcca gtaggtccgg ctctgggtca gcagggcgta aacatcatgg
     1501 aattctgcaa agcgttcaac gcaaaaactg attccatcga aaaaggtctg ccgattccgg
     1561 tagtaatcac cgtttacgct gaccgttctt tcactttcgt taccaagacc ccgccggcag
     1621 cagttctgct gaaaaaagcg gctggtatca agtctggttc cggtaagccg aacaaagaca
     1681 aagtgggtaa aatttcccgc gctcagctgc aggaaatcgc gcagaccaaa gctgccgaca
     1741 tgactggtgc cgacattgaa gcgatgactc gctccatcga aggtactgca cgttccatgg
     1801 gcctggtagt ggaggactaa gaaatggcta aactgaccaa gcgcatgcgt gttatccgcg
     1861 agaaagttga tgcaaccaaa cagtacgaca tcaacgaagc tatcgcactg ctgaaagagc
     1921 tggcgactgc taaattcgta gaaagcgtgg acgtagctgt taacctcggc atcgacgctc
     1981 gtaaatctga ccagaacgta cgtggtgcaa ctgtactgcc gcacggtact ggccgttccg
     2041 ttcgcgtagc cgtatttacc caaggtgcaa acgctgaagc tgctaaagct gcaggcgcag
     2101 aactggtagg tatggaagat ctggctgacc agatcaagaa aggcgaaatg aactttgacg
     2161 ttgttattgc ttctccggat gcaatgcgcg ttgttggcca gctgggccag gttctgggtc
     2221 cgcgcggcct gatgccaaac ccgaaagtgg gtactgtaac accgaacgtt gctgaagcgg
     2281 ttaaaaacgc taaagctggc caggttcgtt accgtaacga caaaaacggc atcatccaca
     2341 ccaccatcgg taaagtggac tttgacgctg acaaactgaa agaaaacctg gaagctctgc
     2401 tggttgcgct gaaaaaagca aaaccgactc aggcgaaagg cgtgtacatc aagaaagtta
     2461 gcatctccac caccatgggt gcaggtgttg cagttgacca ggctggcctg agcgcttctg
     2521 taaactaatg cctttacgtg ggcggtgatt ttgtctacaa tcttaccccc acgtataatg
     2581 cttaatgcag acgtatatcc gagatattcg ggttgtggca aggcggcaac tgagtgagtc
     2641 gccaggagca tagctaacta tgtgactggt gcgaatgaag gaagccaacg ccgccacaag
     2701 ttgaatagcg acggatagaa aagatttgtt cgttggagcc tggcctatcc aggcctccgt
     2761 cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg acagaacgct
     2821 aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac gctctctccg
     2881 tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg agcaaagcta
     2941 atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga agtagccaaa
     3001 ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa aatgactgaa
     3061 ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa caccctgctg
     3121 cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt tggtccgacc
     3181 ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa agagttcgcg
     3241 aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct gatcccggcg
     3301 tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc acgcctgatg
     3361 gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc tgtacgcgat
     3421 gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg tataaactta
     3481 ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat tgaagcagtt
     3541 gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga aaaattcggt
     3601 gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc tgaagaaaaa
     3661 actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt tatcaaagca
     3721 gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggtaga atctgcaccg
     3781 gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa agctctggaa
     3841 gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag cctgagaaat
     3901 caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag gcgccagtag
     3961 cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc gacgacttaa
     4021 tatactgcga caggacgtcc gttctgtgta aatcgcaatg aaatggttta agcgtgatag
     4081 caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt gcacaaactg
     4141 tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta tggtttactc
     4201 ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag ttctggatgt
     4261 accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc aagatcctga
     4321 agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga gctacagcgg
     4381 taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg acgtccagga
     4441 atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc gtctggtgat
     4501 ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag aagtctacat
     4561 gggcgaaatt ccgctcatga cagacaacgg tacctttgtt atcaacggta ctgagcgtgt
     4621 tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca aaggtaaaac
     4681 ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg gttcctggct
     4741 ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc gccgtaaact
     4801 gcctgcgacc atcattctgc gcgccctgaa ctacaccaca gagcagatcc tcgacctgtt
     4861 ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac tggtgccgga
     4921 acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag tgtacgtaga
     4981 aaaaggccgc cgtatcactg cgcgccacat tcgccagctg gaaaaagacg acgtcaaact
     5041 gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact atattgatga
     5101 gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc tgctggctaa
     5161 gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc tggatcacgg
     5221 cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga gcgcactggt
     5281 agaaatctac cgcatgatgc gccctggcga gccgccgact cgtgaagcag ctgaaagcct
     5341 gttcgagaac ctgttcttct ccgaagaccg ttatgacttg tctgcggttg gtcgtatgaa
     5401 gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga gcaaagacga
     5461 catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg aagtcgatga
     5521 tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg aaaaccagtt
     5581 ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc tgggcgatct
     5641 ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag cagtgaaaga
     5701 gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc tgtctgagat
     5761 tacgcacaaa cgtcgtatct ccgcactcgg cccaggcggt ctgacccgtg aacgtgcagg
     5821 cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa tcgaaacccc
     5881 tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg tacgcacaga ctaacgaata
     5941 cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg acgaaattca
     6001 ctacctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact ccaacctgga
     6061 tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg aatccagctt
     6121 gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg tatccgtcgg
     6181 tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga tgggtgcgaa
     6241 catgcaacgt caggccgttc cgactctgcg cgctgataag ccgctggttg gtactggtat
     6301 ggaacgtgct gttgccgttg actccggtgt aactgcggta gctaaacgtg gtggtgtcgt
     6361 tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga tgtatccggg
     6421 tgaagcaggt atcgacatct acaacctgac caaatacacc cgttctaacc agaacacctg
     6481 tatcaaccag atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg acgtgctggc
     6541 agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc gcgtagcgtt
     6601 catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc gtgttgttca
     6661 ggaagaccgt ttcactacca tccacattca ggaactggcg tgtgtgtccc gtgacaccaa
     6721 gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg cgctctccaa
     6781 actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg acattctggt
     6841 tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac tgctgcgtgc
     6901 gatcttcggt gagaaagcct ctgacgttaa agactcttct ctgcgcgtac caaacggtgt
     6961 atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa aagacaaacg
     7021 tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt ctgaagaact
     7081 gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag ccggtggcgt
     7141 tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg gcctgacaga
     7201 cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac tgaaacacga
     7261 gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg atctggcacc
     7321 gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc agcctggtga
     7381 caagatggca ggtcgtcacg gtaacaaggg tgtaatttct aagatcaacc cgatcgaaga
     7441 tatgccttac gatgaaaacg gtacgccggt agacatcgta ctgaacccgc tgggcgtacc
     7501 gtctcgtatg aacatcggtc agatcctcga aacccacctg ggtatggctg cgaaaggtat
     7561 cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac tgcgcgaatt
     7621 catccagcgt gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc tgagtacctt
     7681 cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc caatcgcaac
     7741 gccggtgttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac ttggcgacct
     7801 gccgacttcc ggtcagatcc gcctgtacga tggtcgcact ggtgaacagt tcgagcgtcc
     7861 ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg acaagatgca
     7921 cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg gtaaggcaca
     7981 gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat acggcgcagc
     8041 atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc gtaccaagat
     8101 gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag aatccttcaa
     8161 cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag acgagtaatt
     8221 ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt gctaactccg
     8281 acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact aaaaccgaag
     8341 agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca tggtctttcg
     8401 gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa cgtgacggcc
     8461 ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc ggtaagtaca
     8521 agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg acccagacta
     8581 aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg cacatctggt
     8641 tcctgaaatc gctgccgtcc cgtatcggtc tgctgctcga tatgccgctg cgcgatatcg
     8701 aacgcgtact gtactttgaa tcctatgtgg ttatcgaagg cggtatgacc aacctggaac
     8761 gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc ggtgacgaat
     8821 tcgacgcgaa gatgggtgcg gaagcaatcc aggctctgct gaagagcatg gatctggagc
     8881 aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc aagcgtaaaa
     8941 agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac aaaccagagt
     9001 ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg gttccgctgg
     9061 atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc attaaccgta
     9121 acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga catcatcgta cgtaacgaaa
     9181 aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc ggtcgtgcga
     9241 tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa ggtaaacagg
     9301 gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt tctgtaatca
     9361 ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg gcgctggagc
     9421 tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc accattaaag
     9481 ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg gacgaagtta
     9541 tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg ggtatccagg
     9601 catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg gtttgtgcgg
     9661 catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg acgctggaag
     9721 cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc ccggcgaacg
     9781 gcgaaccaat catcgttccg tctcaggacg ttgttctggg tctgtactat atgacccgtg
     9841 actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa gcagaacgtc
     9901 tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc accgagtatg
     9961 aaaaagatgc aaacggtgaa ttagtagcga aaaccagcct gaaagacacg actgttggcc
    10021 gtgccattct gtggatgatt gtaccgaaag gtctgcctta ctccatcgtc aaccaggcgc
    10081 tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc ggtctgaaac
    10141 cgaccgttat ttttgcggac cagatcatgt acaccggctt cgcctatgca gcgcgttctg
    10201 gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa atcatctccg
    10261 aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg gtaactgcgg
    10321 gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt gtatccaaag
    10381 cgatgatgga taacctgcaa actgaaaccg tgattaaccg tgacggtcag gaagagaagc
    10441 aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt tctgcggcac
    10501 agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc tccatcatcg
    10561 aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac ttcatctcca
    10621 cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aactgcgaac tccggttacc
    10681 tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac gattgtggta
    10741 cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa gagccgctgc
    10801 gcgatcgcgt actgggtcgt gtaactgctg aagacgttct gaagccgggt actgctgata
    10861 tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg gaagagaact
    10921 ctgtcgacgc ggtaatagta cgttctgttg tatcttgtga caccgacttt ggtgtatgtg
    10981 cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caacaaaggt gaagcaatcg
    11041 gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg cgtacgttcc
    11101 acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaagtg aaaaacaaag
    11161 gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa ctggttatca
    11221 cttcccgtaa tactgaactg aaactgatcg acgaattcgg tcgtaccaaa gaaagctaca
    11281 aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct ggcggcgaaa
    11341 ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc ggttttgtac
    11401 gctttactga catgatcgac ggtcagacca ttacgcgtca gacagacgaa ttgaccggtc
    11461 tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa gatctgcgtc
    11521 cggcactgaa aatcgttgat gctcagggta acgacgttct gattccgggt accgatatgc
    11581 ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc gtacagatca
    11641 gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag gacatcaccg
    11701 gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag ccggcaatcc
    11761 tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa cgtcgtctgg
    11821 ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa tggcgtcagc
    11881 tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac ggtccggaag
    11941 cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt tactcgttac atcgttaacg
    12001 aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac atcgaagtta
    12061 tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc gacttcctgg
    12121 aaggcgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg gaagcgaacg
    12181 gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg tctctggcaa
    12241 ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg accgaagcag
    12301 ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc gtgggtcgtc
    12361 tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc cgtgctgcgg
    12421 gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc ctggcagaac
    12481 tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta
    12541 aaaacccgct tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa
    12601 tatttaagga atttctgaat actcataatc aatgtagaga tgactaatat cctgaaactg
    12661 actgaactaa ttgagtcaaa ctcggcaagg attcgatact attcctgtgt aactttctta
    12721 aggaacgaga atgaaacagg aagtggaaaa gtggcgacct tttggacatc cggatggtga
    12781 tattcgtgat ttatcatttc ttgatgctca tcaggctgtc tacgttcagc atcatgaggg
    12841 caaagagcct ttagagtatc gcttttgggt tacctactct cttcactgct tcacaaaaga
    12901 ttatgaacat cagacgaacg aagaaaaaca atcgttaatg taccacgcgc ctaaagaatc
    12961 tcgtcccttc tgccagcacc gttataactt agcgcacaca cttaaaaaga actattttgg
    13021 cgctgccaga aagcaacgtt attcatgccg ggtatggtag ctatgccgtg attgaggtgg
    13081 acttagacgg aggagataag gcattttact ttgttgcgtt cagggctttc agggaaaaga
    13141 aaaaactccg tttgcatgta actagcgctt atcccatttc tgaaaaacag aaaggtaaat
    13201 cagtgaaatt tttcaccatt gcctacaact tattgagaaa taagcagctt cctcagccct
    13261 caaaataaca aaacccacct taaggtgggt ttcgccagag aattatctct ggtattcaga
    13321 acgccattac cggactttgc cttgaccttg cgataatcgc aggttgcggg atgtctgaat
    13381 ttcttcagtc tgctgcatcc tggaagatga gaacatgtgt tcttattttc gtctctatca
    13441 tagttgagta tttactctct tacaatcaga tctctttcat tgctcaacag gcgatggctt
    13501 cagactttgc attacggaat ttttaagaaa ggcagggcga aacgaggaag aagcttttcg
    13561 ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct ttcaacaacc
    13621 ttgagttttt catcttccgt agcggctcgg aataaattta cgttcttaca ttccgtctca
    13681 tagtctttgc gaggcccgtc ccagatagct gtcccagtct ttccataccg gctgcaaacc
    13741 ctgagcggtt aacgcggcag caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg
    13801 ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga aggcgctgac
    13861 gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc gcgtggagag
    13921 tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca ctaactggcg
    13981 ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc gcggaaagga
    14041 gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt cggcaaccat
    14101 atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc ctatcttatc
    14161 aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt cctgtttttt
    14221 tcctttcaga tgatggcggg cataagtcgc ctcgtgatat gtctcctgat aaaccatcac
    14281 gccatccaga ccaagctgct ttaactcggc gtattccgtc tccgccagcg gttgcacttc
    14341 catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa agtaatccat
    14401 ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc ccatctcccg
    14461 tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct tgatgcgatt
    14521 actcatggaa aatccgcagt acgtgcagtc gttagcgcaa agattggaaa gataaagcgg
    14581 gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc
    14641 cagttgttcc agatagccac tggcggctgg cgataacagc gccatcatat cgtcgcgggt
    14701 gagttgcgag gcatttagcg cccgttctac gtcagcagcc gttttgccgt tgatacgcag
    14761 gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc
    14821 tccagaaatc cagtcagcgg gctggtggca tgagcaaaat gactgcggct gcccggtccg
    14881 gactgacgtg ccagcaggcc tgcttctacc gccagacgaa atgccttcgc catgttgacg
    14941 ggatcgtccg cgacggcaat tgccgtattc actaacaccg cgtcggcccc catttccagc
    15001 gcctgcgcgg catggctggg aacgccgatg ccagcatcga caaccaccgg cactgtggcc
    15061 tgctggataa taatctccag catggcgcgg gtttccagtc cctgattcga gccaatcggc
    15121 gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca caataccgga
    15181 tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcgtttc ggcggctttc
    15241 agggtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc taattttaac
    15301 cagtttgtgc ctaacgcttc acgagccaga tgggcggcga aaatggcttc ttccgctgtt
    15361 ttcgccccgg atgtatttgg cagcagggtc acacccgccg cgataagcgg ttcgaggata
    15421 gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg
    15481 gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt gccggtaaac
    15541 agatgtgaat caaacgtttt gtccgcaata cgtaacattt caaccccctg caataacctg
    15601 aaaaagcagg atctggtcgc catcctgcac gatatgttgc gcccactgct cacgcgggac
    15661 gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag
    15721 ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca ggatctgcat
    15781 tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg cgccactggc
    15841 tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt ctctataccg cttaataact
    15901 taatggcttc cagtgcctgc aaagtgccca taaccccgac caccgggcca accacgcccg
    15961 ccgtgcggca gttgcgttct ggctcctggt tatctggcca caggcagcgg taacacccct
    16021 gctcccaggg cggcgtcagt accatcaact gaccgccaaa tccgaccgcg ctggcggtga
    16081 taagcggcgt gttgagtgcc acgcaggcgg cattaatctc ctggcgagtc gccatattgt
    16141 cggtacagtc gagcaccaca tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta
    16201 accgttgttg taatgctgtc agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc
    16261 tgacctgcga tttcgggcga tcgatatctt cagtggtaaa gaggatttgt cgttgcagat
    16321 tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca
    16381 ggtacagcgc agcaggtgta cccagcccgc ccagaccgat aatcagcacc tggctgtcga
    16441 gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag gatttggcgg ctataacgca
    16501 taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc cgttgccaaa
    16561 cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac acccgttgct
    16621 atcaccgcag gcgcgcgtgc cagactgata ccgccaatcg ccacggtggg ataatccgcc
    16681 agtcgctcaa catgccgtgc cagctgttcc agcccctgcg gtgcagaagg catctgtttg
    16741 gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg
    16801 tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc gcggatggca
    16861 ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc ctgatgcttg
    16921 atcgccagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg gcccagcgca
    16981 attgccgcca cgacatcggc ttccacctct tcatcgcgcc gatctttgat gcgtagctgg
    17041 agagtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc caccaccggg
    17101 tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac gcttcctcct
    17161 tacgcaggta gatttctccg cctctggcac ggaagttctc cgacatatcc gccattccca
    17221 tttcaatagt ttgcgtggcg gcgtaatcac gcacttcctg gctgattttc atcgagcaaa
    17281 atttcggtcc acacatggag caaaaatggg cgactttgcc ggactcttgg ggcagggttt
    17341 catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg tcttcccagc
    17401 gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt
    17461 tcgccaggtc agcggcgtgg gcggcaatct tataggtgat aagcccctgc ttaacatctt
    17521 ctttattagg cagacccaga tgctcttttg gcgttacgta acagagcatc gcgcagccaa
    17581 accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat
    17641 cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg
    17701 tcatattgcg gcggatcatc tgcatcggca cgtggcctgg gccttcaatc atcacctgca
    17761 cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg
    17821 cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa
    17881 cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg aaattttcct
    17941 gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg ataccggtca
    18001 ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata gtgaagtaat
    18061 ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt
    18121 cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg atcggcaccg
    18181 ggctgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg gagagatcca
    18241 tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct tcttcgatgg
    18301 aagaggtgac cgcagagttg ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa
    18361 taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca cgtccggcag
    18421 caacttcatc acggacaaat tccgcagtga tattttccgg cagacgtgcg ccaaagctca
    18481 ttcccggatg ctggtggcgt aaaacttcgc tacggatgcg ctcgcggccc atattctcgc
    18541 ggatggcgat gaattccatt tccggtgtga tgatgccctg gcgggcgtag tgcagttggg
    18601 tgacgcggcg tcctgctttg gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt
    18661 cgaggccatc atctgccagc cgcgctttag tgtaatcgga actgcgcacg gtaagttctt
    18721 cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt tgccagccct tgctgcacgt
    18781 taatggcaat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt
    18841 tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta agctggatct
    18901 cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg
    18961 gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc
    19021 gggtcagttt tgttgcagac atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac
    19081 gacggatgaa gcaagagacg atcgccgcag gggcgatgcg atagcagatt aactcttgtt
    19141 cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg tctcagccag
    19201 ttaaggcact ccgacaagaa attagccccg caaatggggc attgaatgta aattacgcgt
    19261 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    19321 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    19381 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca gactggaatc gtagtaatcc
    19441 ataatctgtt gagtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    19501 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    19561 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    19621 tctttgccag gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    19681 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    19741 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19801 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19861 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19921 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19981 atcggcgaat ggcaggggga acctgtttgg ttagtacaac agcagcggcg tcacgatatg
    20041 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgaggc
    20101 gtacaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    20161 tatccgagca aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg
    20221 caaatcgccc cctgcattat tgttgctatc cgtcgcgatg attcgatcct cctcgcccag
    20281 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    20341 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    20401 ttgcgttacg tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg
    20461 gcggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    20521 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    20581 gaagatacgg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    20641 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    20701 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    20761 gaatataaag ccacgcgcgc ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag
    20821 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc
    20881 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20941 gaaggtccgc gttttacctc gccagtcacc tgcaaagctg acgtcgataa actgccaatt
    21001 ccggacccgg aagatgaact gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa
    21061 ctgaaaggcg aagtgtcgct gattggtttt tccggcagcc cgtggacgct ggcgacctac
    21121 atggtggaag gcggcagcag caaagccttc accgtgatca aaaaaatgat gtatgccgat
    21181 ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    21241 gcgcagatta aagccggtgc tcaggctgtg atgattttcg acacctgggg cggcgtgctt
    21301 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    21361 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    21421 tggctggaag cgatggcaga aaccggttgc gatgcgctgg gcctcgactg gacaacggat
    21481 atcgccgatg cgcgccgccg cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg
    21541 tcgatgctgt acgcgccgcc agcccgcatt gaagaagaag tagcgactat acttgcaggt
    21601 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    21661 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc
    21721 taaggagtga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct
    21781 gtgatccgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg agccgatgtc
    21841 gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa atatccctcg
    21901 cttgagctgg tggagtataa agtggcccgc atcgccacca ccatgcctta cattccaggt
    21961 tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag
    22021 ccggatttag tgtttgtcga tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc
    22081 agccattttg gcttattggt ggatgtgccg accattggcg tggcgaaaaa acggctctgc
    22141 ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc
    22201 gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc
    22261 ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat
    22321 cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg
    22381 cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg tatttgagaa
    22441 ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag ctggcagcat
    22501 gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat gttctgccag
    22561 caaaccggtt ttggtgatgg gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg
    22621 ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt tgaagaagcg
    22681 attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg
    22741 gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa
    22801 gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa
    22861 atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga cattcagtgg
    22921 gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt
    22981 agggcagacc tgcgtgaggc gggtgatagc aatattggta taatttttca gcaataagac
    23041 cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt cgccccccgt
    23101 ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc
    23161 tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt gtaaggataa
    23221 cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact gtccaaaacc
    23281 caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct gaaagaaggc
    23341 gatgctgtac aactggttgg tttcggtacc ttcaaagtga accaccgcgc tgagcgtact
    23401 ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt accggcattt
    23461 gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt gaacagtttt
    23521 aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat gctggcaggt
    23581 gcgctgtttc tcactgcctg tagccacaac tcttcacttc ctccctttac cgccagtgga
    23641 tttgctgaag accagggcgc ggtacgcatc tggcgaaaag acagcggcga taatgtgcat
    23701 ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg
    23761 cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt
    23821 cgcgcgcgtt ttgacgatcg cggtgatctg agctttatgc aacgtgaatc cgatggggaa
    23881 aagcagcagc tttctaacga ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc
    23941 cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg
    24001 atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt agattcgcag
    24061 gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg
    24121 ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt ctgtcgctgg
    24181 caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga ccgccaccac
    24241 cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct tcagccatcg
    24301 cgatatcccg tttcacccgt aactcatcta acgactgacg caaattctcc atctctttgg
    24361 cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca ttgtattcat
    24421 aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag tcattatgga
    24481 ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac
    24541 cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa agcgccatca
    24601 ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt gtcttacttc
    24661 gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat ctggcatgat
    24721 aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc attgaaactt
    24781 aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat ttctgctgtc
    24841 agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta aagactcctt
    24901 agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag ggttgtttgc
    24961 ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa cgctgctgga
    25021 aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca tggggatgcg
    25081 catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc ctggagtacg
    25141 ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact ccggcatggt
    25201 gggaaaacag ctttattccc cgcaggaaat gcagcagtta catccgggag atgaagaagc
    25261 gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc gccagtttca
    25321 accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg ccgcgacacc
    25381 acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag atcgtgagca
    25441 gagaaacacc ctgattatcc tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc
    25501 attcttctgg tatcgccgct atctgcgctc gcgccagctt ctacaagatg aaatgaagcg
    25561 caaagagaag ctagtggcgc tggggcatct tgcggcaggc gttgcccacg aaatccgtaa
    25621 cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg
    25681 agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt
    25741 aagcgagctg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg tggatctcaa
    25801 cacgctgatt aaccactcat tacagctggt aggccaggat gcaaacagcc gggagatcca
    25861 gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg acaggctgac
    25921 tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc atggcgtgat
    25981 tagcgtgacg gtcagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa
    26041 gggaattgcg gcagatcagc ttgaagccat cttcactccg tacttcacca ccaaagccga
    26101 aggcaccgga ttggggctgg cggtcgtgca taatattgtt gaacaacacg gtggtacaat
    26161 tcaggtcgca agccaggagg gaaaaggctc aacgttcacc ctctggcttc cggtcaatat
    26221 tacgcgtaag gacccacaag gatgacgcac gataatatcg atattctggt ggtggatgat
    26281 gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta taacgtcgcg
    26341 ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg agcgggtttt tgatcttgtg
    26401 ctttgcgatg tgcgaatggc agagatggac ggcattgcca cgctaaaaga gatcaaagcg
    26461 ttaaatccgg cgattccggt actgattatg accgcatact ccagcgtcga gacggcggta
    26521 gaggcactga aaaccggggc gctggattat ctcatcaagc cgctggattt cgacaccctg
    26581 caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgatgctga aacgcctgcg
    26641 gtgactgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt
    26701 gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga ttccggcacc
    26761 ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga aaaaccgctg
    26821 gtaacgctca actgtgtggc actcaacgaa tccttgctgg aatctgaatt gttcggtcac
    26881 gaaaaagggg cgtttactgg agccgataaa cgccgggaag ggcgctttgt tgaggcggac
    26941 ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg
    27001 ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac tatctcggtt
    27061 gatgtccggc tgattgcggc gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt
    27121 tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg
    27181 caacggcggg aggatatccc tctgctggct agccattttc ttcagcgttt tgccgagcgt
    27241 aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat ccactacgac
    27301 tggccgggaa acattcgtga actggaaaac gcggtggaaa ggtcggtggt gctgctgacc
    27361 ggggaatata tttccgaacg cgagctgccg ctggcgattg ccagtacgcc gatcccgctg
    27421 gggcaaagtc aggatattca gccgctggtg gaagtcgaaa aagaagtgat tctggcggca
    27481 ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa
    27541 acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata
    27601 tctttccggc agaagcagtc gtcccagtga atatcggtca ttaaggcata agcgcgtttc
    27661 tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc
    27721 actacctgct cgttatccgc cagtttcgtg cccgcgtgga acactttgcc gcctgccact
    27781 tcttccagcg gcaggccgtg gatcacgtca ccggtgcggt aatcacccgg atatccaccc
    27841 gcagccatca ccacgccgag agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt
    27901 ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg cagcataatc
    27961 ggctgggttt ctggatcgcc aaagcggcag ttaaattcga taaccttcgg attgccctgt
    28021 ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg
    28081 cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc atcggttact
    28141 accggcgcgg gggagtaagc gcccatcccg cccgtgtttg gtccggtatc tttatcgcct
    28201 acgcgtttgt gatcctggct ggtggccatc ggcagcacat gctcgccgtc taccatcacg
    28261 ataaagctcg cttcttcgcc atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg
    28321 ccaaaagcgt tgcccgccag catatcgtga acagccgctt ccgcttcttc cagcgtcatc
    28381 gccacgataa cgcctttccc ggcagccaga ccgtccgctt taatgacgat tggcgcgcct
    28441 ttctcacgca gatacgccag cgcaggttct acctcggtga agttctggta ttccgccgta
    28501 gggatcttat ggcgggccag gaaatctttg gtaaacactt ttgagccttc cagttgggcc
    28561 gcacctgcgg ttgggccgaa gattttcagc ccggcggcgc ggaaggtatc gaccacgcct
    28621 ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa
    28681 tccaacagcg ccgggatatc ggtcacgcca atagcaacgt tttgcagcgc gggttccagt
    28741 gcagtgcctg cattacccgg agcaacaaaa acagtctcaa ccagcggcga ctgggccgct
    28801 ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt catctattgc
    28861 tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg
    28921 cggcggcaat cacttcgtca tcacggatgg aaccgccagg ctggattacg caggtcacgc
    28981 ccgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg
    29041 aggaaccttt cacttccagg ccttcatcgg cagctttaat accggcgatt ttcgcggagt
    29101 acacgcggct catctggccc gcgccaatac cgatggtcat attgtttttg gcatagacga
    29161 tagcattgga tttcacgaac ttcgccactt tccagcagaa cagcgcatcg cgcagttcct
    29221 gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc atccccaggt
    29281 cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct
    29341 cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg attttcaggg
    29401 cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa atgatggcct
    29461 gtgcggtttc cgcatctagc tcgcggttaa aggcaatgat gccgccgaat gcggaggttg
    29521 ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata gctacaccgc
    29581 aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc acgcactcca
    29641 gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga acctgggttg
    29701 cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc tgctggtggc
    29761 tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc gtgcgtggga
    29821 agcgaccagc ggcttctttg ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt
    29881 tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac
    29941 gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg gcgtcgtagt
    30001 cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag cgcaccatgg
    30061 tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcaacct tcacgggcca
    30121 cggtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg atctgatgtt
    30181 cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt actttcggat
    30241 gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg
    30301 ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac agcagctcca
    30361 caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca gaaacactga
    30421 gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctggat ttgactatta
    30481 cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa cggggcatcc
    30541 ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt
    30601 caatggtgat cacaattttg acagtggtta ccgtgggaaa aatacagaaa ttacattgat
    30661 gattgtggat aactctgtgc gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc
    30721 agtcagtcat ttttctgcaa tttttctatt gcggcctgcg gagaactccc tataatgcgc
    30781 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZUFB010000052        29637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZUFB010000052 DAZUFB010000000
VERSION     DAZUFB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.052"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(107..2176)
                     /gene="glyS"
                     /locus_tag="ACHY3M_002635"
     CDS             complement(107..2176)
                     /gene="glyS"
                     /locus_tag="ACHY3M_002635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4317529.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2186..3097)
                     /gene="glyQ"
                     /locus_tag="ACHY3M_002636"
     CDS             complement(2186..3097)
                     /gene="glyQ"
                     /locus_tag="ACHY3M_002636"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4317530.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3192..3491)
                     /gene="ysaB"
                     /locus_tag="ACHY3M_002637"
     CDS             complement(3192..3491)
                     /gene="ysaB"
                     /locus_tag="ACHY3M_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFS4317531.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3666..4661
                     /gene="wecH"
                     /locus_tag="ACHY3M_002638"
     CDS             3666..4661
                     /gene="wecH"
                     /locus_tag="ACHY3M_002638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFS4317532.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4703..5140)
                     /gene="yiaA"
                     /locus_tag="ACHY3M_002639"
     CDS             complement(4703..5140)
                     /gene="yiaA"
                     /locus_tag="ACHY3M_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFS4317533.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5186..5527)
                     /gene="yiaB"
                     /locus_tag="ACHY3M_002640"
     CDS             complement(5186..5527)
                     /gene="yiaB"
                     /locus_tag="ACHY3M_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFS4317534.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5696..7150)
                     /gene="xylB"
                     /locus_tag="ACHY3M_002641"
     CDS             complement(5696..7150)
                     /gene="xylB"
                     /locus_tag="ACHY3M_002641"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4317535.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7222..8544)
                     /gene="xylA"
                     /locus_tag="ACHY3M_002642"
     CDS             complement(7222..8544)
                     /gene="xylA"
                     /locus_tag="ACHY3M_002642"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFS4317536.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELMLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8910..9902
                     /gene="xylF"
                     /locus_tag="ACHY3M_002643"
     CDS             8910..9902
                     /gene="xylF"
                     /locus_tag="ACHY3M_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4317537.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9980..11521
                     /gene="xylG"
                     /locus_tag="ACHY3M_002644"
     CDS             9980..11521
                     /gene="xylG"
                     /locus_tag="ACHY3M_002644"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFS4317538.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11499..12680
                     /gene="xylH"
                     /locus_tag="ACHY3M_002645"
     CDS             11499..12680
                     /gene="xylH"
                     /locus_tag="ACHY3M_002645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFS4317539.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12758..13936
                     /gene="xylR"
                     /locus_tag="ACHY3M_002646"
     CDS             12758..13936
                     /gene="xylR"
                     /locus_tag="ACHY3M_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFS4317540.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14044..14868)
                     /gene="bax"
                     /locus_tag="ACHY3M_002647"
     CDS             complement(14044..14868)
                     /gene="bax"
                     /locus_tag="ACHY3M_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFS4317541.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15188..17218
                     /gene="malS"
                     /locus_tag="ACHY3M_002648"
     CDS             15188..17218
                     /gene="malS"
                     /locus_tag="ACHY3M_002648"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS4317542.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17396..18649
                     /gene="avtA"
                     /locus_tag="ACHY3M_002649"
     CDS             17396..18649
                     /gene="avtA"
                     /locus_tag="ACHY3M_002649"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFS4317543.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18801..19274)
                     /gene="ysaA"
                     /locus_tag="ACHY3M_002650"
     CDS             complement(18801..19274)
                     /gene="ysaA"
                     /locus_tag="ACHY3M_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4317544.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19376..20224)
                     /gene="yiaJ"
                     /locus_tag="ACHY3M_002651"
     CDS             complement(19376..20224)
                     /gene="yiaJ"
                     /locus_tag="ACHY3M_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFS4317545.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20425..21423
                     /gene="yiaK"
                     /locus_tag="ACHY3M_002652"
     CDS             20425..21423
                     /gene="yiaK"
                     /locus_tag="ACHY3M_002652"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFS4317546.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21435..21902
                     /gene="yiaL"
                     /locus_tag="ACHY3M_002653"
     CDS             21435..21902
                     /gene="yiaL"
                     /locus_tag="ACHY3M_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFS4317547.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22020..22493
                     /gene="yiaM"
                     /locus_tag="ACHY3M_002654"
     CDS             22020..22493
                     /gene="yiaM"
                     /locus_tag="ACHY3M_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFS4317548.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22496..23773
                     /gene="yiaN"
                     /locus_tag="ACHY3M_002655"
     CDS             22496..23773
                     /gene="yiaN"
                     /locus_tag="ACHY3M_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFS4317549.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDIQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23786..24772
                     /gene="yiaO"
                     /locus_tag="ACHY3M_002656"
     CDS             23786..24772
                     /gene="yiaO"
                     /locus_tag="ACHY3M_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFS4317550.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24776..26272
                     /gene="lyxK"
                     /locus_tag="ACHY3M_002657"
     CDS             24776..26272
                     /gene="lyxK"
                     /locus_tag="ACHY3M_002657"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFS4317551.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26269..26931
                     /gene="ulaD"
                     /locus_tag="ACHY3M_002658"
     CDS             26269..26931
                     /gene="ulaD"
                     /locus_tag="ACHY3M_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4317552.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKVLS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            26924..27784
                     /gene="sgbU"
                     /locus_tag="ACHY3M_002659"
     CDS             26924..27784
                     /gene="sgbU"
                     /locus_tag="ACHY3M_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4317553.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27778..28473
                     /gene="araD"
                     /locus_tag="ACHY3M_002660"
     CDS             27778..28473
                     /gene="araD"
                     /locus_tag="ACHY3M_002660"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4317554.1"
                     /translation="MLEQLKADVLAANLELPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28505..28606)
                     /locus_tag="ACHY3M_002661"
                     /pseudo
     CDS             complement(28505..28606)
                     /locus_tag="ACHY3M_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28610..28873
                     /locus_tag="ACHY3M_002662"
                     /pseudo
     CDS             28610..28873
                     /locus_tag="ACHY3M_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28820..29560)
                     /gene="yiaT"
                     /locus_tag="ACHY3M_002663"
     CDS             complement(28820..29560)
                     /gene="yiaT"
                     /locus_tag="ACHY3M_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFS4317555.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
BASE COUNT         7307 a         7601 c         7436 g         7293 t
ORIGIN      
        1 tatatgagat aatctttatt cagcagaaga ttattaaagg ttgctgtatt atttagcgat
       61 aataaaaaaa gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg
      121 aaatatccgc aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga
      181 gacgcaattc tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct
      241 cacgcagctc tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct
      301 ccagcttgtc acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca
      361 gggtagaggc attcacgcgg tcactcagca cttcgtcaga tttcgccaaa atgttggaga
      421 cacgcttgtt cgccgccgcc agtgcagcag ctgcttcaag ggtacggaag tgcgataccg
      481 ctttcatgcg ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga
      541 tggtgtcaac ggtgtaacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt
      601 cgataacatc atcaaccacg ttggcgttag tcagcttatc gccatacaga cgcactgctt
      661 cttcggtcag cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc
      721 caagtgcggc acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tggccgatac
      781 cgaagatacc cgccagagta tccatcttgt cagcaatcgc cagcgcacag gctaccgggt
      841 tagacggcag gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gccaccgcga
      901 catcttccgc ttcgccatcg tgacgcgcat agtgcatccc cataacgccc tgggtgtcgg
      961 tgaactcgaa gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg
     1021 cgtggttaac gtcagcgcca atctgttcag caatccagcc agccagcgcc tggatgcggt
     1081 cagttttgtc gcgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca
     1141 ggttatcttc cagacgtttt ttacggtcgg tattgaagaa gaactcggca tccgccagac
     1201 gtggacgaac gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt
     1261 tggcaacgaa gatgaagttc ggcagcagtt tgccatcgtt cgcatacacc gggaagtatt
     1321 tctggtcacc tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt
     1381 cgaattttgc ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc
     1441 tttcgcttaa gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa
     1501 tcttcgcttt acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg
     1561 gatactgatc ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa
     1621 tcacgcgatc ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca
     1681 gggtcacggt gtgcaccgga cgcacgaagt gcacgtcgct tgcgccccaa cgcatcagtt
     1741 tcgggatcgg cagtttcgcc agagaagtcg caaccatatt cggcagcagt gcttcggtgc
     1801 tttcgccctt cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct
     1861 cagcctggtc aacggtaata ccgcaaccac gcgcccaacc ttctgccgct ttgctcggtt
     1921 tgccttcagc gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatccg
     1981 gttgcgcttc cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt
     2041 gaacggtgcc gtgtgcgagg ccagcgttat ccagctccgc agtaaaattc gcagcaaagg
     2101 actcagccag gctgcgcagt gcttttggtg gcagttcttc agtgccgatt tccaccagaa
     2161 aagttttctc agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg
     2221 cttcacggga agcgtagtat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa
     2281 tgtagcgctg acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt
     2341 gggcggcttt cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca
     2401 gctgctgcgc ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt
     2461 attcgaagtt gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg
     2521 ttttacccag cgggccgtcg ctccagacca ggtcgtaaac gctgtctacg ccctgaatgt
     2581 acatggccag acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac
     2641 caccaacctg ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc
     2701 agcccagtcc ccaggcaccc agagtcgggt tttcccagtt atcttccacg aaacggatgt
     2761 cgtgaatagt cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat
     2821 tgtccggcga tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt
     2881 tttcgccgta gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg
     2941 gttctggccc cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca
     3001 tgtccaatgg ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga
     3061 tcaagccctg gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt
     3121 ggatttaaag gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga
     3181 aaaagcaggg cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag
     3241 acaaactgct ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg
     3301 aaggcggtga cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg
     3361 cataacgctt ccatactcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc
     3421 acgggtgacg gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg
     3481 ttcatcatca ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata
     3541 gtggctgata tcttacgaat ttccttaagc ctctataagc tgtagtgata aaactcagat
     3601 tattccagtg cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga
     3661 cacagatgca gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg
     3721 tggtgatgat tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca
     3781 catgggatat cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca
     3841 tgatttccgg ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta
     3901 tcggcttatg tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct
     3961 ccatcaatat ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt
     4021 ggtttttctt cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg
     4081 tcggcggaaa aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca
     4141 cagtgccgca gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg
     4201 atacttttta ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac
     4261 agcataaagc actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct
     4321 ctcgcgggac attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt
     4381 actgtgggcc gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc
     4441 tggatacgcg taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg
     4501 gattccacgc cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc
     4561 caatactgga tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta
     4621 tgctggtaca acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt
     4681 ggactgaccg ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt
     4741 ttggcgggtt tattccggta tcacgaatat tcttctgcac cgctactgca ccaaacaagc
     4801 ttaagaagaa agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca
     4861 gacctaccat cagtaatgca acagagataa taaagacagt caggcaggtc atataataaa
     4921 tggaagtggt cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag
     4981 aaaacagtcc taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg
     5041 cattccatag ccctaacaga taggtaacga taccaccaac gagagctatc catgacacaa
     5101 tactaaaggc cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc
     5161 tgaatgtgta agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca
     5221 agcagacaaa aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta
     5281 aaacaccgat aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa
     5341 aaaaattatc cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg
     5401 ctgtcattaa cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata
     5461 gcccaaccag ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg
     5521 ttttcataac gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa
     5581 actgaaaaat gccattaaca aatgattttc agaataaatt catactaaat attaattaat
     5641 tagtgagata tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg
     5701 ccattaatgg cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag
     5761 cggcataacg ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact
     5821 caatgagcga tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg
     5881 gccccacatc cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct
     5941 gacgccagta ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc
     6001 cgcaggcatg cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg
     6061 ctcgcgccag ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct
     6121 ggggattatt gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg
     6181 cactttcatc agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta
     6241 atttcgcgac ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac
     6301 gctgcggtag cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt
     6361 cgctgacagc aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa
     6421 ccattcccac accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg
     6481 tcgccatacc ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt
     6541 cgtataatgc gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc
     6601 agtcacgctt tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa
     6661 actcccccgt catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac
     6721 ggaatatctc cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca
     6781 tcatcaggtt gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact
     6841 cttgcgcaca gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat
     6901 ccagtaaggt tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag
     6961 aatgctgatc gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt
     7021 cttgttccga ccagagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa
     7081 ccacctcacc ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc
     7141 cgatatacat atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc
     7201 aacggactgc acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag
     7261 ttgctcctgg cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc
     7321 taaatctgcc agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc
     7381 ggaataacgc tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat
     7441 tttcagcgcc actgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt
     7501 atcagtactt tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc
     7561 tgctttgaga atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc
     7621 ggtgtcccag cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag
     7681 cgcaatggcg gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc
     7741 ttcaatgttc agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt
     7801 cgcggcatcg taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa
     7861 cgtgccctgg aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag
     7921 ttgttcacgc tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc
     7981 ccacaggaca tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg
     8041 cgttgccgcc cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg
     8101 agggtttgta aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt
     8161 ggctgccagg acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc
     8221 ctcaggggaa acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa
     8281 ctcaaatgcg acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca
     8341 cggacgatta aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca
     8401 gtagcaggcg gcaaaacgca agtgctcttc catacgctta cccaacatca gttcgtcggg
     8461 attgtagtga cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc
     8521 gagctggtca aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg
     8581 gatgatgtcg taatattggg cactcccttt cagttgctca attatgttat ttcacactgc
     8641 tattgagata attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg
     8701 gtaattcctc gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg
     8761 tcataaatca agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt
     8821 cccctgttta gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc
     8881 tgtcctctaa ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt
     8941 gcacctcact tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg
     9001 cgattgatga tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg
     9061 cagaatctct cggcgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa
     9121 tgtcgcagat tgaaaacatg ataaaccggg gtgttgatgt tcttgtcatt attccgtata
     9181 acggtcaggt attaagtaac gttgtaaaag aagccaaaca agaaggtatt aaagtattag
     9241 cttacgatcg tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa
     9301 aagtcggtga actgcaggca aaagccctgg tcgatattgt tccgcaaggt aattacttcc
     9361 tgatgggcgg ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag
     9421 tgttaaaacc ttacgttgat tccggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg
     9481 gctggttacc ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca
     9541 aaattgatgc tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa
     9601 gcgcgcaagg tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta
     9661 ttaaacgtat tgctgccggt acgcaaacta tgacggtgta taaacctatt acgttgttgg
     9721 caaatactgc cgcagaaatt gccgttgagt tgggcaatgg tcaggaacca aaagcagata
     9781 ccacactgaa taatggcctg aaagatgtgc cctcccgcct cctgacaccg atcgatgtga
     9841 ataaaaacaa catcaaagac acggtagtta aagacggatt ccacaaagag agcgagctgt
     9901 aagcgttacg ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat
     9961 tggcttaggt ggagtcgtta tgccttatct acttgaaatg aagaatatta ccaaaacctt
    10021 cggcagtgtg aaggcgattg ataacgtctg cttgcggttg aatgctggcg aaatcgtctc
    10081 actttgtggg gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta
    10141 tccccatggc tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca
    10201 catccgcgat accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa
    10261 agaattgacc gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat
    10321 ggattatgac ctgatgacgc tacgctgtca gaagctgctc gcacaggtca gtttatccat
    10381 ttcacctgat acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc
    10441 caaggcactt aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac
    10501 tgagcaggaa acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc
    10561 ctgtatttat atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt
    10621 tattcgcgac ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat
    10681 tatcaccatg atggtcgggc gagagttaac cgcgctttac cctaatgaac cacataccac
    10741 cggagatgaa atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat
    10801 taaacgagtt aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg
    10861 actcgttggt gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca
    10921 atgggaagga aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc
    10981 catcgcccag gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt
    11041 aatggcggtt ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag
    11101 ccagcttgat gacgcggaag agcaaaaatg tattctggaa tcaattcagc aactcaaagt
    11161 taaaacgtcg tcccccgatc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc
    11221 gatcctcgct cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag
    11281 gggtatcgat attggcgcga aatacgagat ctacaaatta attaaccaac tcgtccagca
    11341 gggtattgcc gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg
    11401 ggtactggtg atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca
    11461 ggagcaggtg atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg
    11521 aagtgaaatt ggccgtaccg acatccggtg gcttttccgg gctgaaatca ctgaatttgc
    11581 aggtcttcgt gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg
    11641 atggtgccta cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg
    11701 gcatcctcgc ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg
    11761 gctcaatgat ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc
    11821 ctttgccact taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg
    11881 gatggtgggt cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg
    11941 catttcgcgg catactcatt ggcatcacca acggcacgac tgtttccccc accagcgccg
    12001 cgatgtcaca aattgggcaa agctatctac ctgccagcac tggcttcatc attggcgcgc
    12061 ttggcttaat ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg
    12121 gtttgcagtc tccggcctct accgcagtag tcggtcgcca ggctttaacc gctatcatcg
    12181 tattaggcgc aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc
    12241 tgacattgct gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca
    12301 tttatgccat cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca
    12361 ccaaacttgc cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta
    12421 gttctcgact tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg
    12481 cagcatgcgt gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag
    12541 taatgggggc atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga
    12601 ccttctggca gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg
    12661 caaccaaacg ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg
    12721 gttttgagtt tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat
    12781 cacattactg ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga
    12841 atatttacag gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg
    12901 cattgataaa atcaaggact ggttaggaga tggcgtcatt gccgacttcg acgacaaaca
    12961 gatcgagcaa gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca
    13021 ccttgcagaa agttacccac ccgttcatta cattgccacc gataactatg cgctggttga
    13081 aagcgcattt ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc
    13141 ggaatcaagc ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc
    13201 cgaagaaaag tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca
    13261 acatgcgcaa aatcggctgg cagactggct acaaacgctg ccaccgcaaa ccgggattat
    13321 tgccgttact gacgcccggg cgcggcatat tctgcaagta tgtgaacatc tacatattcc
    13381 cgtaccggaa aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc
    13441 gcgtgtcgcc ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa
    13501 actgttgcat cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc
    13561 agttcgcgtc attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat
    13621 tcaggccatg cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact
    13681 ggatgcggtc gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga
    13741 aaccatccat gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc
    13801 aaccaccttg tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt
    13861 ctactctgtt tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag
    13921 cgaggtcatg ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag
    13981 ccacctgatg cgagcaaaag ccttatccga ccgacaaatc agataaggct caaggaaatg
    14041 caattacata tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt
    14101 gttataactc ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt
    14161 cacttcctga tccgctttac gcagctgcgc acgcgattta cggaatgaag agtaagccgg
    14221 atgcgtattg aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga
    14281 gtaccctttc actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa
    14341 cagattgttg ttgttgcgcg ccagctttga cgttccccaa ccgctttctg ctgcagccat
    14401 cgtcgccacc atactggtgg ggataatgtc tacgcgttca agcaaggtat tccacgggat
    14461 ttttcgcgta ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc
    14521 acgctcagca ggtgaccatt ggccctgata ctgttttgaa atcagccagt tacgttccgc
    14581 agtaatggct gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt
    14641 ccttggtgtt ccggaagggt attttcgcaa atcaggaagt gaattactct ttgcactatt
    14701 gcgagaatac tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt
    14761 ttgactcgct gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag
    14821 taacataaga atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg
    14881 tcggatttaa tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat
    14941 catatcaaaa atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca
    15001 cgagttaacg attcagattg gcgctaaatc gcagaaaatg tggggtttat cgcaaaattc
    15061 agccgttttt tgcgcgagat cgctcaccct tgcttctcat cctgtggact taccgctcag
    15121 ggatgagttt tgtttggctt atcgctggca aactgtctga aatcgcagca ataaggactc
    15181 atccgccatg aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc
    15241 cagctggact tctccggggt tccctgcctt tagtgaacag ggaacgggaa catttgtcag
    15301 ccacgcgcag ttgcccaaag gtacgcgtcc actaacgcta aattttgacc aacagtgctg
    15361 gcagcctgcg gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac
    15421 gcagcctcaa tggcgattgt tcagggacgg cgaatatacg ctgcaaatag acacccgctc
    15481 cggcacgcca acattgatga tttccatcca gaacgccgcc gaaccggtag caagccaggt
    15541 ccgtgtatgc ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc
    15601 ggaaggagcc gccgtacggg attattacag ccagcaaatt gcgatagtga agaacggtca
    15661 aataacgtta caacccgctg ccaccagcaa cggtttactc ctgctggaac gggcagaaac
    15721 tgacgcctct gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg
    15781 tttcgaaaac ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc
    15841 ggaaattggc acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct
    15901 ccagcagttg ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg
    15961 ggtcggcggc ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca
    16021 ggactggacg aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga
    16081 tagcgcacat cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccggcta
    16141 tgccacgctg gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga
    16201 ggtgaaaaaa acgctgggtg aacgctggag cgactggaaa cctgcagccg ggcaaacctg
    16261 gcatagcttt aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg
    16321 aaaaaactgg atccgtaccg atatcggcga ttacgacaat cctggattcg acgatctcac
    16381 catgtcgcta gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc
    16441 ggtgttctat aaaaacaaaa cggataccca cgctaaagcc atcgacggct ttacccctcg
    16501 cgattactta acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg
    16561 ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag
    16621 cgccgcgctt cgcgaatgga aaaaagctaa ccccgacaaa gcattagatg acaaaccttt
    16681 ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg
    16741 cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagctg tcgattgtat
    16801 tgcgcagatg gatatgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt
    16861 gagctacctc tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga
    16921 gttattacta ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc
    16981 gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga
    17041 tgttagcggt aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc
    17101 ccgccatccc gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg
    17161 ctttgttcgt gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact
    17221 tttccggctt cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc
    17281 ctaatcagcg ttgcaggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag
    17341 caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac
    17401 attctccctt tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga
    17461 tctgaacgac ggtttacgca cccctggcgc gattatgctc ggcggcggta atccggcgca
    17521 gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa
    17581 agcgactgat gcactgtgca attacgacgg tccacagggg aaaacggagc tactcacact
    17641 gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact
    17701 aacaaacggc agccagagcg cgtttttcta cttatttaat ctgtttgccg gacgccgtgc
    17761 cgatggtcgg gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga
    17821 cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga
    17881 aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat
    17941 gatttgcgtc tcccggccga cgaatccaac aggcaatgtg attactgacg aagagttgct
    18001 gaagcttgac gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg
    18061 cgtcccgttc ccgggtatca tcttcagtga agcgcgcccg ctatggaatc cgaatatcgt
    18121 gctgtgcatg agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc
    18181 caatgaaaaa atcatcaccg ccatcaccaa tatgaacggc attatcagcc tggcacctgg
    18241 cggtattggt ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc
    18301 tgaaacagtc atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg
    18361 ccgctattta ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg
    18421 gctatggttt aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg
    18481 cggcgtgctg atggtgccgg ggcacaactt cttcccaggg ctggataaac cgtggccgca
    18541 tacgcatcaa tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt
    18601 gaagattctg gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga
    18661 tgcgctacgc ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt
    18721 aggccggata aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg
    18781 agaaacgccg gaaaaacgtt tcaaacaggc tgctgccgta gccgatgccg ctgcactttc
    18841 tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggtcccgtc
    18901 tcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc
    18961 tccatcgcac caaacgggca agccagcata cagcttttac agccaatgca acgtgtttgt
    19021 tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac
    19081 ggtgcatctt cacactgatg acaggctact gccgtggtcc agctgtggtc tttaatgaca
    19141 cgaatacggg aaataaactc gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc
    19201 gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata
    19261 ataaaccggt tcatgatcac tcctttgcac taaaacagca gagtgccaga gagcgcgtcc
    19321 ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg
    19381 tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg
    19441 tttcacgcag tggtttcagg agatttttct ctcccacctg tttcagacgt gatgtcgaaa
    19501 gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac
    19561 aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg
    19621 ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct
    19681 catgctgatg gttttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga
    19741 tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca
    19801 gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg
    19861 agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat
    19921 gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg gtagtcaggc
    19981 ggtaactccc tgcggcgggc gcggtggtca catagccgca ggactgtaat ccctgcaata
    20041 agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac
    20101 cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc
    20161 cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc
    20221 ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat
    20281 aatttataac cacttgattt ttatgtcttt tgaaattcat caatcagatt gctttgttaa
    20341 aaagtgatcg atatatttga aatcaagttt cgcatattga gattttaagc caaaaaagcg
    20401 atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg agcagttaaa agcagccttt
    20461 aatcgggtct taatttcacg cggcgttgac agcgaaacgg ctgacgcctg tgcagagatg
    20521 ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg ttaatcgttt ccctcgtttc
    20581 attcaacaac tggaaaacgg cgatatcatt cctgatgccc aacccaaacg tataaccagc
    20641 ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg gcaacctgac ggcgaaaaag
    20701 atgatggatc gcgccattga actggctgcc gatcacggta ttggtctggt ggcacttcgt
    20761 aatgccaacc actggatgcg cggcggcagc tacggctggc aggcggcgga aaaaggctat
    20821 attggcattt gctggaccaa ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt
    20881 cgcattggca ctaacccgct gatcgtcgcc attccttcca cgccgatcac catggtcgat
    20941 atgtcgatgt cgatgttctc ttacggcatg ttagaagtta accgcctggc gggccgtcag
    21001 ctcccggtcg atggtggctt tgatgatgag ggcaatttga ccaaagaacc tggcgttatc
    21061 gagaagaatc gccgcatttt gccgatgggc tactggaaag gttctggcat gtcgattgtg
    21121 ctggatatga tcgctactct cctttccgac ggcgcatcgg ttgccgaagt cacccaggac
    21181 aacagcgacg aatacggcat ttcacaaatt tttattgcca ttgaagtgga caagcttatc
    21241 gacggtccca cccgcgatgc caagctgcaa cgtatcatgg attacgttac taccgctgaa
    21301 cgcgctgacg aaaaccaggc catccgctta cccggccacg aatttactac cctgctggcc
    21361 gaaaaccgcc gtaacggtat taccgttgat gacagcgtgt gggccaaaat ccaggcgtta
    21421 taaggagata agtcatgatt tttggacata tcgcgcagcc caatccgtgc cgtttgcccg
    21481 ccgccattga aaaggcgctc gattttctgc gcgctaccga tttcaacgcc ctggagccgg
    21541 gcgttgtcga aatcgacggc aaaaatattt atgcgcaaat tatcgattta accacacgcg
    21601 aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat cgatattcag tttctggcgt
    21661 ggggtgaaga gaaaatcggc attgctattg atacgggaaa taataaagtc agcgaatcac
    21721 tattagagca gcgcgatatt attttttatc atgacagtga acatgaatca tttatagaaa
    21781 tgataccggg cagctacgca attttctttc cgcaggatgt tcaccgacca ggttgtattt
    21841 tgcaaacagc ctctgagata cgaaaaattg tggttaaagt cgcattaacg gcgctgaatt
    21901 aatcgaataa tcgtctacgc cagaacgact gggtaatgtt attgctctaa ctatattaat
    21961 aatttaggtt gctgcacatg caacttgaat tatgacagac atagcctcaa ggaatagcta
    22021 tgaaaaaaat acttgaagca atactggcga ttaatctcgc cgtactttcc tgtattgtgt
    22081 ttataaacat tattttaaga tatggttttc agacaagcat tttatctgta gatgaattgt
    22141 cacgttattt atttgtctgg ttaacattta ttggcgcgat tgtggctttt atggataacg
    22201 cccacgttca ggtcaccttt ctggtggaga aactctcccc cgcatggcaa cgacgagtcg
    22261 ctctggttac ccattcctta atcttattta tttgtggcgc actggcctgg ggcgcaacgc
    22321 taaaaacaat tcaggactgg agcgattatt caccgatcct cggtttaccc atcggcctga
    22381 tgtatgccgc ttgtctgccc accagcctcg ttatcgcatt ctttgaactg cgtcatttat
    22441 atcaactcat cacgcgtagc aattctctaa cgtcgccacc gcaaggagct taatcatggc
    22501 tgtgctgatt tttctgggct gtctgttggg tggtattgct atcggtttgc ctattgcctg
    22561 ggcactgttg ttgtgcgggg cggcgttaat gttctggctg gacatgtttg atatccagat
    22621 catggcacaa acgctggtga acggagccga tagcttctcc ctgctggcga ttccgttctt
    22681 tgtgctggcg ggtgaaatca tgaatgcggg cggcttgtca aaacgcattg ttgacctgcc
    22741 gatgaaactg gtggggcata aaccaggcgg gctgggctac gtcggcgtgc tggcagctat
    22801 gattatggcg agcctttctg gttctgccgt tgcggatacc gccgccgttg ccgctctact
    22861 ggtgccgatg atgcgcagcg ccaactaccc ggtcaaccgg gcggcggggc tgattgcctc
    22921 tggcggcatt atcgcgccaa ttattccccc ttccattccg tttattatct tcggcgtttc
    22981 cagcggatta tccatcagta agctgtttat ggcgggcatt gcccctggca tgatgatggg
    23041 cgcaacgctg atgcttacct ggtggtggca ggccagtcgc cttaatttac cccgccagca
    23101 aaaagcaacg atgcaggaaa tctggcactc ttttgtctcc ggtatctggg cgctgtttct
    23161 tccagtcatc attattggcg ggtttcgctc cggtctgttt acccctaccg aagccggggc
    23221 cgttgctgct ttctatgcgc tgtttgtcgc cacagttatt taccgtgaaa tgaccttcgc
    23281 cacactttgg catgtactca ttggcgcggc gaaaaccacc tcagtggtta tgtttctggt
    23341 ggcctcggca caagtttccg cctggctgat taccattgct gaactgccga tgatggtttc
    23401 tgatttactg caaccgttgg tcgattcacc gcgtctgctg tttatcgtca ttatggtggc
    23461 tatcctgatt gtcggcatgg tcatggattt aacgccgacg gtattaattc ttaccccggt
    23521 attaatgcct ttagttaaag aggcaggaat cgatccgatt tatttcggtg tcatgtttat
    23581 cattaactgc tcaatcggct taattacacc gcctatcggt aatgtactta acgttatttc
    23641 aggggtggca aaactcaaat tcgatgatgc agtcagaggc gttttccctt acgtcctggt
    23701 tttatattca ttattagtcg tatttgtttt tattcccgat ctcatcatcc tccctttgaa
    23761 atggattaat taaaaggaaa atattatgaa attacgctct gtaacctacg cattattcat
    23821 tgctggcctg gctgcattca gcacatcttc tctggcggcg caatctttac gtttcggtta
    23881 tgaaacctca caaaccgact cgcaacatat tgcggcgaaa aaattcaatg atttattgca
    23941 ggagagaacc aaaggcgagc tgaaattaaa attgtttccg gatagcaccc tcggtaacgc
    24001 gcaggcgatg atcagcggcg tacgtggcgg caccatcgat atggaaatgt ccggctcgaa
    24061 taactttgcc gggttatcac cagtgatgaa cttgcttgat gtccctttcc tgttccgcga
    24121 taccgctcac gcgcataaaa cgctcgacgg caaagtcggt gatgatctga aagcctcact
    24181 tgaaggtaaa ggactaaaag tactggccta ctgggaaaac ggctggcgcg atgtcaccaa
    24241 ctcgcgcgca ccggttaaaa cccccgccga cctgaaaggg ctgaaaattc gcaccaacaa
    24301 tagcccgatg aatatcgccg cattcaaagt ctttggcgct aacccgatcc cgatgccgtt
    24361 tgccgaagtc tataccgggc tggaaacccg cactatcgac gctcaggaac acccgatcaa
    24421 cgtcgtctgg tcagcaaaat ttttcgaagt gcagaagtac ctttctctga cgcaccacgc
    24481 ctattccccg cttctggtgg tgatcaacaa agcgaagttt gatggcttaa gcccggagtt
    24541 ccagcaagcg ctaatttcat ctgcgcagga agcgggtaac taccagcgca aactggttgc
    24601 cgaagatcag caaaaaatca tcgacggcat gaaagaagcg ggcgtggaag tcatcaccga
    24661 tctcgaccgc aaagccttta gcgacgcact gggtactcag gtccgcgaca tgtttgtaaa
    24721 agatgttccg cagggtgctg atctactgaa agccgtggat gaggtgcaat aaaccatgac
    24781 gcaatactgg ctggggttag attgtggcgg tagctggctg aaagccgggc tgtatgaccg
    24841 cgaaggccgg gaggcaggcg tgcagcgcct gccgctgtgc gcattaagcc cgcagccagg
    24901 ttgggcagag cgcgatatgg cagaactgtg gcaatgctgc atggctgtca ttcgcgaact
    24961 gcttacccat tccggcgtta gcggggaaca aattgtcggt atcggtatct ccgcacaagg
    25021 aaaaggcttg tttttgctgg ataaaaacaa caagccgctc ggtaacgcca ttttatcctc
    25081 ggatcgccgg gcgatggaaa tcgttcgtcg ctggcaggaa gatggcatcc cggaaaaact
    25141 ctacccgctg acccgacaaa ccttgtggac cgggcatccg gtatcgctgt tacgttggct
    25201 taaagagcac gaaccggaac gctacgcgca aattggctgc gtgatgatga cgcacgacta
    25261 cctgcgctgg tgtttaaccg gcgtcaaagg ctgtgaagag agcaatattt ccgagtccaa
    25321 tctctacaac atgagtcttg gggaatatga cccgtgcctc accgactggc tggggattgc
    25381 tgaaatcaat cacaccctgc cgcctgttgt cggatctgcc gaaatctgcg gggagatcac
    25441 cgctcagaca gccgtactga ccggtctgaa agcgggtacg cccgttgtcg gcggcctgtt
    25501 tgatgtggtt tccaccgcac tctgcgccgg gatcgaagac gaatttaccc ttaatgcagt
    25561 gatgggcacc tgggcggtga ccagcggcat aacccgcggt ttacgtgacg gtgaagcgca
    25621 tccgtatgtc tatggtcgct acgttaacga tggtgaattt atcgttcacg aagccagccc
    25681 cacctcttcc ggcaacctcg aatggtttac cgcacagtgg ggagaaatct cgtttgatga
    25741 gatcaaccag gccgttgcca gcttgccgaa ggccggtggc gatctctttt tcctgccgtt
    25801 cctgtacggc agcaacgccg gactcgagat gaccagcggt ttctacggga tgcaggccat
    25861 tcacacccgc gcacacctgt tgcaggccat ttatgaaggg gtggtgttca gccatatgac
    25921 ccatctcaac cggatgcgcg aacgttttac tgatgtgcac accctgcgcg tcactggcgg
    25981 cccggcgcat tccgatgtct ggatgcaaat gctggcggac gtcagcggtc tgcgtattga
    26041 gctgccgcag gtggaagaaa ccggctgctt tggtgcagca cttgccgccc gcgtcggcac
    26101 cggggtttat cgcgatttca gcgaagccca gcgtgattta cagcacccgg tgcgcaccct
    26161 gctgccggat atgactgtcc atcagcttta ccaacaaaaa taccaacgct atcagcatct
    26221 cattgccgca cttcagggct ttcacgcccg catcaaggag cacatattat gagccgacca
    26281 cttctgcaac tggcactcga ccactcatca cttgaagccg cacagcgcga cgtgacgcag
    26341 ttaaaagaca gcgtcgatat tgtcgaagcg ggcaccattc tctgtttaaa cgaagggctt
    26401 ggcgcggtga aagccttgcg cgaacagtgc cctgacaaaa tcatcgttgc cgactggaag
    26461 gtcgccgacg ctggtgaaac gctcgcgcaa caggcatttg gcgcaggcgc taactggatg
    26521 accatcatct gcgccgcacc gctcgcgacg gtggaaaaag gccacgcgat ggcgcaacgc
    26581 tgcggtggtg aaattcagat cgagctgttc ggtaactgga cgctggacga cgcccgcgac
    26641 tggcatcgta ttggcgtgcg gcaggcgatt tatcatcgcg gtcgtgatgc acaggccagc
    26701 gggcaacagt ggggcgaagc cgatctggca cgcatgaagg tgctttccga tatcggcctt
    26761 gagctttcca ttaccggcgg gattacccct gctgacctgc cgctgtttaa agatattcgc
    26821 gtgaaagcgt ttattgccgg gcgagcactg gcaggctcag caaatccggc gcaggtggct
    26881 ggcgatttcc atgcgcaaat cgacgctatc tggggaggca agcgtgcgta atcatccgtt
    26941 agggatttat gaaaaggcgc tggcgaaaga tctctcctgg ccggagcggc tggtgctggc
    27001 aaaaagctgc ggttttgatt ttgtcgaaat gtcggtggat gaaaccgacg aacggctttc
    27061 acgtcttgac tggagcaccg cacaaaggac ttcgctggtt gccgcgatga tcgaaacggg
    27121 tgttgggatc ccgtcgatgt gcctgtccgc acatcgtcgc tttccttttg gtagccgtga
    27181 cgacgcggta cgccagcgag cacgggagat catgagcaaa gcgattcgtc tggcacgcga
    27241 tctcggcata cgcaccatcc agctggcggg ctacgacgtc tattacgaag accacgatga
    27301 aggcacccgg caacgttttg ctgaagggct ggcgtgggcg gtcgaacagg cggcggcatc
    27361 acaagtcatg ctagcggtgg agattatgga caccgcgttt atgaactcca tcagtaaatg
    27421 gaaaaagtgg gacgagatgc tcgcctcgcc gtggttcacc gtctacccgg acgtcggcaa
    27481 cctcagcgcc tggggcaatg atgttcccgc cgaactgaaa ctgggcattg accgtatcgc
    27541 cgcgatccac ctgaaagaca cccagccagt taccgagcaa agtcccggac agttccgcga
    27601 cgtgccgttt ggcgaaggct gcgtcgattt cgttggcatc ttcaaaacgc tgcataaact
    27661 gaactatcgc ggttctttcc tgattgagat gtggaccgaa aaagccaaag agccggtgct
    27721 ggagattatt caggcgcggc gttggattga agcgcgtatg caggaggctg gatttatatg
    27781 ttagagcaac tgaaagccga cgtgctggcg gcgaatctgg agcttcccgc tcaccatctg
    27841 gtgacgttca cctggggcaa tgtcagcgcg gtagacgaaa cgcggcaatg gatggtaatc
    27901 aaaccttccg gcgtcgagta tgacgtgatg accgccgacg atatggtggt ggttgagata
    27961 gccagcggca aggtggtgga aggtagcaaa aagccctcgt ccgacacgcc aacgcatctg
    28021 gcgctttacc gtcgctacgc cgaaatcggt ggcattgtgc atacccactc acgccacgcc
    28081 accatctggt cgcaggccgg gctggatctc cccgcctggg gcaccacgca cgctgattat
    28141 ttctatggtg ccatcccctg cacgcgacag atgaccgcag aggagattaa cggcgaatat
    28201 gaatatcaga ccggcgaagt gatcattgaa accttcgaag aacgtggcag gagtccggca
    28261 caaatcccgg cggtgctggt gcattctcac ggcccgttcg catggggtaa aaacgccgcc
    28321 gatgccgtgc ataacgccgt agtactcgaa gaatgcgcct atatgggcct attctcgcgc
    28381 cagcttgcgc cgcagctccc tgcgatgcaa aacgaactgc tggataagca ctacctgcgt
    28441 aagcatgggg ccaatgccta ttacgggcag taatccctca cgccggggct tcatcgcccc
    28501 ggcactacga attgatatgt tccttgctgt aacgccgctt ccacgctgct ggcgttaaac
    28561 cggtgtgctt gcgaaaaatc tgccggaaat agccaacgtc attaaaccgt attgcatgga
    28621 gcaggccgac aacgtcgagt cactgcataa cctgttgctg cccgccgatg cgccatttac
    28681 gacatttgaa ggcaagggat tgttcagcca taagatcctg attcagacgc caggttcccg
    28741 gtatgaacag agcaatccag agcaacaacc tttgtggcat tacgacggag caccagccgc
    28801 accgacatcc accggtgaat taaaaacgcc agctcacgcc ggtgataaag gtaaaactct
    28861 cattacgatc aatcatcggg ctgttggtaa tctcttccgg cagctcgctg tatcctgcgc
    28921 tcgccatcaa tacgacgtgc tctcctatcg ggtattttgc cgtcaggctg acatagggca
    28981 cccaggcatc ctgcgcggaa taacttgcca gaccgctacg acgggactca ctctctgaaa
    29041 tgccatagta atagtcactg aaattctcgt cataatagag aacgcccagc gcaggtgtca
    29101 gcgacaggcg acctatctgc attttgtgga ataccgatag ctcccccacc cagccgttgc
    29161 tgttatccag aacgtccgca gccgcagagg ctttcacggt tccccaacgc tcgtggtgat
    29221 accaggcaac ccccgccata gccgtactat cacgcttatc aagctgttgc atggcataat
    29281 cgtcattatc ggcagggtca aattccagcg gcatccagga tgcggtcagg ctaagttcat
    29341 ttttttcact ttgcgacaga ataaaaccta atgtggtctg acgaacataa aaagaatcac
    29401 cttcataact aatcagcgga attgcctttg tattttcatt ataaccgcga taaggcgatt
    29461 cattataagc tgcaccagca ccaatggata attcagaagc agttgcgctt gccataaaag
    29521 gcaacgcaaa taacgccaca atattgcgat taattaacat gtccggtatt ccattttaaa
    29581 ataagataaa aaagagtcgg cataataatg cttacaattt ctccggtgca agattca
//
LOCUS       DAZUFB010000053        29576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZUFB010000053 DAZUFB010000000
VERSION     DAZUFB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.053"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(249..995)
                     /gene="cutC"
                     /locus_tag="ACHY3M_002664"
     CDS             complement(249..995)
                     /gene="cutC"
                     /locus_tag="ACHY3M_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4317556.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1009..1575)
                     /gene="yecM"
                     /locus_tag="ACHY3M_002665"
     CDS             complement(1009..1575)
                     /gene="yecM"
                     /locus_tag="ACHY3M_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4317557.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1791..3524
                     /gene="argS"
                     /locus_tag="ACHY3M_002666"
     CDS             1791..3524
                     /gene="argS"
                     /locus_tag="ACHY3M_002666"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4317558.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3566..3610)
                     /gene="yecV"
                     /locus_tag="ACHY3M_002667"
     CDS             complement(3566..3610)
                     /gene="yecV"
                     /locus_tag="ACHY3M_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFS4317559.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3701..4189
                     /gene="yecT"
                     /locus_tag="ACHY3M_002668"
     CDS             3701..4189
                     /gene="yecT"
                     /locus_tag="ACHY3M_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFS4317560.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4309..4701)
                     /gene="flhe"
                     /locus_tag="ACHY3M_002669"
     CDS             complement(4309..4701)
                     /gene="flhe"
                     /locus_tag="ACHY3M_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFS4317561.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTSVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4701..6779)
                     /gene="flhA"
                     /locus_tag="ACHY3M_002670"
     CDS             complement(4701..6779)
                     /gene="flhA"
                     /locus_tag="ACHY3M_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS4317562.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6772..7920)
                     /gene="flhB"
                     /locus_tag="ACHY3M_002671"
     CDS             complement(6772..7920)
                     /gene="flhB"
                     /locus_tag="ACHY3M_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFS4317563.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8122..8766)
                     /gene="cheZ"
                     /locus_tag="ACHY3M_002672"
     CDS             complement(8122..8766)
                     /gene="cheZ"
                     /locus_tag="ACHY3M_002672"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFS4317564.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8777..9166)
                     /gene="cheY"
                     /locus_tag="ACHY3M_002673"
     CDS             complement(8777..9166)
                     /gene="cheY"
                     /locus_tag="ACHY3M_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFS4317565.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9181..10230)
                     /gene="cheB"
                     /locus_tag="ACHY3M_002674"
     CDS             complement(9181..10230)
                     /gene="cheB"
                     /locus_tag="ACHY3M_002674"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFS4317566.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10233..11093)
                     /gene="cheR"
                     /locus_tag="ACHY3M_002675"
     CDS             complement(10233..11093)
                     /gene="cheR"
                     /locus_tag="ACHY3M_002675"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFS4317567.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11112..12716)
                     /gene="tap"
                     /locus_tag="ACHY3M_002676"
     CDS             complement(11112..12716)
                     /gene="tap"
                     /locus_tag="ACHY3M_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFS4317568.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12762..14423)
                     /gene="tar"
                     /locus_tag="ACHY3M_002677"
     CDS             complement(12762..14423)
                     /gene="tar"
                     /locus_tag="ACHY3M_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFS4317569.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14568..15071)
                     /gene="cheW"
                     /locus_tag="ACHY3M_002678"
     CDS             complement(14568..15071)
                     /gene="cheW"
                     /locus_tag="ACHY3M_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFS4317570.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15092..17056)
                     /gene="cheA"
                     /locus_tag="ACHY3M_002679"
     CDS             complement(15092..17056)
                     /gene="cheA"
                     /locus_tag="ACHY3M_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFS4317571.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17061..17987)
                     /gene="motB"
                     /locus_tag="ACHY3M_002680"
     CDS             complement(17061..17987)
                     /gene="motB"
                     /locus_tag="ACHY3M_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFS4317572.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDLISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17984..18871)
                     /gene="motA"
                     /locus_tag="ACHY3M_002681"
     CDS             complement(17984..18871)
                     /gene="motA"
                     /locus_tag="ACHY3M_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS4317573.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18998..19576)
                     /gene="flhC"
                     /locus_tag="ACHY3M_002682"
     CDS             complement(18998..19576)
                     /gene="flhC"
                     /locus_tag="ACHY3M_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFS4317574.1"
                     /translation="MSEKSIVQEAWDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19579..19929)
                     /gene="flhD"
                     /locus_tag="ACHY3M_002683"
     CDS             complement(19579..19929)
                     /gene="flhD"
                     /locus_tag="ACHY3M_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFS4317575.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20709..21137
                     /gene="uspC"
                     /locus_tag="ACHY3M_002684"
     CDS             20709..21137
                     /gene="uspC"
                     /locus_tag="ACHY3M_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFS4317576.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21144..22568)
                     /gene="otsA"
                     /locus_tag="ACHY3M_002685"
     CDS             complement(21144..22568)
                     /gene="otsA"
                     /locus_tag="ACHY3M_002685"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFS4317577.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22543..23343)
                     /gene="otsB"
                     /locus_tag="ACHY3M_002686"
     CDS             complement(22543..23343)
                     /gene="otsB"
                     /locus_tag="ACHY3M_002686"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFS4317578.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGADLEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23510..24496)
                     /gene="araH"
                     /locus_tag="ACHY3M_002687"
     CDS             complement(23510..24496)
                     /gene="araH"
                     /locus_tag="ACHY3M_002687"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFS4317579.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24511..26025)
                     /gene="araG"
                     /locus_tag="ACHY3M_002688"
     CDS             complement(24511..26025)
                     /gene="araG"
                     /locus_tag="ACHY3M_002688"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFS4317580.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(26095..27084)
                     /gene="araF"
                     /locus_tag="ACHY3M_002689"
     CDS             complement(26095..27084)
                     /gene="araF"
                     /locus_tag="ACHY3M_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFS4317581.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27881..28384
                     /gene="ftnB"
                     /locus_tag="ACHY3M_002690"
     CDS             27881..28384
                     /gene="ftnB"
                     /locus_tag="ACHY3M_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFS4317582.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKSMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28463..28714)
                     /gene="yecJ"
                     /locus_tag="ACHY3M_002691"
     CDS             complement(28463..28714)
                     /gene="yecJ"
                     /locus_tag="ACHY3M_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFS4317583.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28829..28915)
                     /gene="azuC"
                     /locus_tag="ACHY3M_002692"
     CDS             complement(28829..28915)
                     /gene="azuC"
                     /locus_tag="ACHY3M_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFS4317584.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29178..29501
                     /gene="yecR"
                     /locus_tag="ACHY3M_002693"
     CDS             29178..29501
                     /gene="yecR"
                     /locus_tag="ACHY3M_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFS4317585.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         7305 a         7895 c         7031 g         7345 t
ORIGIN      
        1 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
       61 atcccataat caatgtgaat ataaaaaata ttattagcac aacgctatgt tcactgtcac
      121 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
      181 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      241 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      301 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
      361 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      421 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc ggccataatg
      481 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
      541 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
      601 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
      661 gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc gagaacgccc
      721 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
      781 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
      841 atcgtcaccc gctggcgcac ggatttcagt acccccaacg acggcgttaa gcccccctct
      901 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
      961 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     1021 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     1081 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     1141 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1201 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     1261 acgtttttcc ccgggccacg gtagctccac aatagaaaac tgccagtgcg caacctgtac
     1321 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt ctgacaaaag
     1381 ctcgccacac tgttcaaacc cgcgacgcca gcgttcagca gtggcgtttt gatggcaacg
     1441 caaagaaatg tgatcggcag tcagcggagt gatattcaac cccagacgga gggaaagttc
     1501 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1561 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1621 cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1681 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     1741 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     1801 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     1861 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     1921 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     1981 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     2041 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     2101 gtctcggtgt ttctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2161 tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
     2221 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2281 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2341 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2401 aagagttcgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2461 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
     2521 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
     2581 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2641 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     2701 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     2761 gttatgaatc actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     2821 acctaatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     2881 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     2941 cgggtggtac agtgaaactg gccgatctgc tggatgaagc cctggaacgt gcacgccgtc
     3001 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
     3061 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3121 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
     3181 acacgcgtgt attgtccgtg ttccgtaagg cagaaattaa cgaagagcaa ctggctgcag
     3241 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3301 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtgatgtgt gcttacctgt
     3361 acgatctggc tggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3421 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctaaagc
     3481 tgggtctgga tacgctgggt attgagacag tagagcgtat gtaatcgatt tttcgtgaga
     3541 gtaaagcctg atcagggtta cctgatcagg cttttttatt gccatctaaa tgtattctga
     3601 aaatggacat gccattgttt tctgactgtt ggataagagg ccagaagcgt aatatccggc
     3661 cccagggaaa cgataacggt tgaatttaag gaataccgca gtgtttaaat ttcttgtatt
     3721 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
     3781 tgacatttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
     3841 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     3901 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgtaa aaaaacctga
     3961 taccgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt ttagggacaa
     4021 agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtgacc cttattctga
     4081 atcagaatgg ctctcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4141 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatcttt
     4201 ttggcatgtt ttgttgcaag ctattcctga taaataattg caacaagaca tcgagccttt
     4261 ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc agcgataatt
     4321 cacaatcact tcattacgtt gtacctttag cggtggaatt aaccgcccac cgcctggcac
     4381 ttcccagata aatcgcaacg gttctgctgc cgctataccg gaaaaggcca cggaggttcc
     4441 gctctgcccc tctaattcga cacaacgaga ttgcgaacac aagcgaaccc gcagtcctga
     4501 aggtgtcggg ccgataagct gataacgcca cgctaccagc gtcatcaatc ctgaagcagg
     4561 ttgtcgcgta gaaagaggcg cagacgacat cgactcaccg cgatgattta gcgtaattcc
     4621 tacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa
     4681 tattgctaat aaggttctca ttatttgccg ccaattgtcg ccgtcatgcg gatatgtcgg
     4741 ttatcagaca gttccagatt cgacaggacc actaactgcg gcaagctgcg gcgcaggaag
     4801 cgagacaata atggtcgcag cgcgtggttc accaacaata ctggcggcgc acccagcatc
     4861 tcctgacggg atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc
     4921 agtcctcccc cgccctgcag cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca
     4981 ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg tcccaacgcc
     5041 acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc
     5101 gtttcgagaa tggtgcgcat atcgcgaatc ggtacttttt catcgaggag attttgcagc
     5161 actttatgca gtgtggtgag cgtgacgacg ccaggaacga gatcttccgt cagctttggc
     5221 atctcctggg cgacgcgatc caacagctgt tgcgcctcct gacgaccaaa cagctctgcg
     5281 gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc ctcaaccact
     5341 gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat agcattcagg
     5401 ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt tccagggtta
     5461 atcgccagcc agcgccccgg ataagcatca ccactgccaa tctccacgcc tttcatcaaa
     5521 atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga
     5581 aatcccatct cctgggcaaa tttcttgcga atactgcgta tacggcccaa caactcacca
     5641 tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc cagagaatct
     5701 tccagttgta catcgttcca cgtcgcttcg acaacggtat tattctctgc catttttacc
     5761 ggtttgggtt cgacaggcgc tttttgttcg cgtccgcgta tccaccaggc cagccccagc
     5821 aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag gccgagtaaa
     5881 ccgagcacgg cggcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc
     5941 tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa
     6001 atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa tggtcaatag cgtataactt
     6061 tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa cccgccgaca
     6121 acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc
     6181 ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg ttttttcgcc
     6241 tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg cttacccggc
     6301 ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc tttggtaatg
     6361 accataaagt tgatgatcac gagaatgaca aacactacga taccgatagc gaaattgcca
     6421 ccaacgagga agtgaccgaa tgcctcgacc accttcccag ccgccgccgc gccggtatgc
     6481 ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa caacgtagta
     6541 aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
     6601 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
     6661 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
     6721 tgccattgtg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag attactcatg
     6781 ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac
     6841 agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg ccagcacttc
     6901 cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccgcat gtcgatacag
     6961 cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg
     7021 aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt
     7081 ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga catccgcttt
     7141 cggcacatcg gccatcatcc gacgccgtgc agcagctcgc tgcatctgac ggatccgccc
     7201 tttaacatga gggtcacctt cgctttgttt gaactcatca cgaatatcct gccgtgacat
     7261 acgcagcttt ttcaggtggc tgaatatttg gaaaaagacg tcaaatccca ccattggaat
     7321 gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat tacccatggc
     7381 ggtaatcgga gactcggcca tcaagcgcat catctgcggc cagtgatgcc agagaaaaaa
     7441 ccccgtcacg ctgccaacca ggatggtttt caaaattgct ttaagcaact ccgcgccagt
     7501 ctgagccgag aacatccgtt taatgcccgg tagcgggttg agtttggaaa acttcggctg
     7561 caaggatttg ccgctaaata ccagccctcc cagcatgacc ggagaaatga gcgccaccag
     7621 caccacgccg ctaatcagcg gcagcagcgc cagcatggct tctctgatca gcagaataat
     7681 ctgcccgagg atcagattcg ggtcattgat aatactgtga tcaaaatgca gcccagcgga
     7741 gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa cactaacgcc
     7801 caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc cctcttcccg
     7861 cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac
     7921 gtcgccaatc ctggaaagag tattaagcgt gaatgatgcc agagcgcaaa gcgttcaatg
     7981 gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gctgccacac atcaagcata
     8041 gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac cgcctgatat gacgtggtca
     8101 cgccacatca ggcaatacaa atcaaaatcc aagactatcc aacaaatcgt ccacctgatc
     8161 ctgactggct accacaccgg ctttgctggt atcgacctga ggtccattaa gcaaactctg
     8221 gttttcacgt tttggacgcg actcctgttc cgggatgttt tccaacagca ccatcagcaa
     8281 ctggcgttcg atctcctgaa tgacatccat catccgctta atgacctgcc cggtgagatc
     8341 ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg ttagtaaagc tggtatgcgc
     8401 gggtacatct gccagaaatt gtcgtgtatc tgttaccagt tcacgggcgt cggcaaggtc
     8461 aatcggatcg gcaaaccagt catcccaacg ttgggttaac gcttttgctg atttctccat
     8521 ttgatcctga tgcggttgtg acgcctcaac actgttcagc gcccgctccg cagcctgggc
     8581 ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg gggatggctt ccgccgcttc
     8641 ggcaatggcc tgatccagcc ccagttcccg caaactgtcg cgcagcatac gcgtcaggct
     8701 gccgatgcgc gcaatgatat cgccagctga atgctcgtca gcaggtttga ttgatggttg
     8761 catcatagtc gcatcctcac atgcccagtt tctcaaagat tttgttgagt ttttcctcca
     8821 gcgtcgcggc ggtaaatggc ttcaccacat agccactggc ccccgcttgc gccgcagcaa
     8881 tgatgttctc tttcttcgct tctgcagtca ccattaacac tggcaatgcc gacatcgcgc
     8941 catccgcacg aattgttttc agcaattcca ggccatccat attgggcatg ttccagtcgg
     9001 agataacaaa tccataaccg cctgcctgca acttattgag agcgtcgacg ccatcttccg
     9061 cttcctcaac attattgaat cccagctctt tcagcaggtt acgcactatg cgtcgcatgg
     9121 tggaaaagtc atccacaacc aaaaatttaa gttctttatc cgccatttca cactcctgat
     9181 ttaaatacgt atcgcctgtc cggcactaat ttttgccaac atttgctggc ttacctggct
     9241 aagatcgacc acttcgcaga caccacccat attgatggcc tcgcgcggca tgccgaacac
     9301 cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc gcctgacgca tcgccaacat
     9361 tcccgccgcg ccgtcgttgc ccataccggt caggatcacc ccaaccgcat tacgccccgc
     9421 ctgtttggcg acagaatgga acaacacatc taccgaaggc cgatgacggt taaccgccgg
     9481 gccatcgtga attttgattt ggtaatttgc gccactacgc gacagctcca tatgccgatc
     9541 gcccggcgca atataggcat gccccggcaa gacacgttct ccgtcttcgg cttctttaac
     9601 cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat
     9661 atgttgggta attaacaatg ccgggctgga aagcggcaac ggttgcagta cgtgacgaat
     9721 tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg
     9781 acccgccttc agcgttgtcg gtgctgacaa tggcttatgt gctgcaaggc tcgcctttgc
     9841 tgccgtacgc accttttcag caatcatttc gctatacgcc agcattcctt cgcgaatacc
     9901 cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca gcgtgacttc
     9961 tgatcctttg ccggtcaagg aagaaaccat cacaacgggc attggacgca aacgcattaa
    10021 tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg tcagcacatc
    10081 gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat
    10141 ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc
    10201 atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc atacaccgtc
    10261 tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt tttcagagtg acccgcaaac
    10321 agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg ctgggtattt
    10381 tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc cggcacggtg
    10441 tactgtttcg ccagtagatt cagcggggca aaatcaacat agttcgccag ctcctgacgc
    10501 acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg cagttgctgc
    10561 ggcgtcaggt ttttcaactc ttcatggcga taaataccgc ttctggcttt ttccagcact
    10621 tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca
    10681 gccagcgtca tcgcaatgct gtacggctct tcgccggtcg aagccgccgc gctccatacg
    10741 cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg gtgtgcctca
    10801 cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca ctcaccgctg
    10861 tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc
    10921 aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat ggtcagccag aacgatcccg
    10981 gctcgttgat agatcaattg acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
    11041 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
    11101 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
    11161 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
    11221 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggcgttctg ctgagtcacc
    11281 tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc cccgctgttg ttcttccgac
    11341 gcagaggcaa tttctcccat aatgtcgtcc acgcgggtca ccgaactgac aatatcgatc
    11401 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
    11461 tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac ggctggcaag attgcgcact
    11521 tcacctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttccactgcc
    11581 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
    11641 ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt gagtcatggt actgacctga
    11701 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca gttccgacgc ctgtcgtgcg
    11761 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
    11821 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
    11881 gcgataccaa tgtgcatctc ctggcttccc ttacgcacat cactcaccgt cccacgcaaa
    11941 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
    12001 gcaatcggac gcgccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
    12061 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
    12121 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
    12181 ctggcggctt ccagcacatg gttgatctcc agctgccagg cctcaaagtt aacgtcaaac
    12241 gcattctgtg attcctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
    12301 caggtcgcct gatgttcgag atcgttgttg tgccagcggg caaaactttt ctccgtctct
    12361 ttttgcaacg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact tttaaacagc
    12421 gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat gtcatccgcc
    12481 ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg cggtactggc ctgtaacatt
    12541 accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac ctgattcagt
    12601 cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat ctgcaagatc
    12661 ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt aaacatcaac
    12721 gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt ttcccagttt
    12781 ggagcttgtt cagcaattcg cagtcgtggc tgtgccggtg gttgctcact ggcaggacgg
    12841 gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgc ggaaaccgct
    12901 tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg cggcagctga ttcctgcacc
    12961 agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc gacttgatcg
    13021 atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc agtcacgcga
    13081 gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag caccgaaccg
    13141 gtatcaacgc gtgagacgga gtcttcaatg agggctttga tctcttttgc cgcctgggcg
    13201 ctgcgactgg caagattacg cacttcaccc gccaccacgg caaaaccacg gccctgttca
    13261 cccgcacgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg gaaggcaata
    13321 ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat ctcatgcatc
    13381 gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga ggcactttgc
    13441 gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc ggtgagctgc
    13501 tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg ggaggaaaga
    13561 tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga accttcgcgg
    13621 acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg cgccaggtcg
    13681 cccatttcac tgcgcccgtc aatggtcagg gtattcgcca agttaccacc ggcgatttcg
    13741 cgaatgtgag caataatttt tgccagcgga gtaagcaaca tacggcgaat gccgtaccac
    13801 gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca ctgggcaaat
    13861 cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact gctgagggcg
    13921 tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg agcgaaataa
    13981 gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt gtgatagttt
    14041 ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg tgccatgctt
    14101 ttgaattttt tataatgagt cgcggcctgg gccaatgttt tcctggcgct atcgagcaat
    14161 tcaactttgg cgttactttg ttgattggag gaatccatca tcatccgcac cgctgaacga
    14221 ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc gccctgctgt
    14281 tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga agaaaaaaac
    14341 agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac caacagcgtg
    14401 actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttaat atcgttatcg
    14461 gcaagaaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa ataaaaaacc
    14521 cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc gggagaatta cgccacttct
    14581 gacgccgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc gatgttcacc
    14641 agaatcaaca tccggtcgcc cagtgcgccc agtccagtga gatattctgt tgaaagcgtc
    14701 acggcaaact ctggtgccgg acgaatttgc tccgccgtca atgaaagcac gtctgaaacg
    14761 ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac taccgtgttg
    14821 tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat cggcacaata
    14881 acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat ccgtgttacc
    14941 tgatcgtagc cacggatctc ctgcactttc aggatatcaa taccgtactc ttcatcacca
    15001 agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt tacattcgtc
    15061 ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc atacgttgtt
    15121 cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg ccgtcgccaa
    15181 gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg tttttaacca
    15241 caacctggtg ctgaccaatt aattgatcca ccagcaaggc gtagcggcga ccgccacttt
    15301 gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg ttgaacactt
    15361 tccacagttc gacgatgggc agatattcac cccgcacttc cagcacccgc tcgccgccgg
    15421 ccagtggatg cagatcggct tcacggggtt gcagtgattc cataacagca ttcagcggca
    15481 gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc gaggatggcc agcgtcagcg
    15541 gcagtaaaat gcggatcgta gtgccagtac cctgcttcga ctggatttcg acatgaccgc
    15601 ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccgcgcccg gagacgtcgg
    15661 tgacctgctc tgccgtggag aagcctggtg caaatatcag catcgcgact tcgtcgtcgc
    15721 tcatgttttc gctgacagtc aaaccttgcg aggccgcttt tgccagaatt cgctcacggt
    15781 ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga tgttcggcag
    15841 acagaattaa atttccgacg ctgtttttac ctgcggcgag ccgtttttct ggcagttcaa
    15901 taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg cgttctatca
    15961 ggcttttgtc gagttcagta gaactgccca ccagcgtcag ttctacctgc ttgccgagtt
    16021 ttcccgccag atcacgcacc agccggggat agcgactaaa aacatattcc atcggcatca
    16081 tgcgaatcga catcactgat tcctgcaaat cacgggcgtt acgttgtaac tgccccatgc
    16141 tggttatcaa atcaccatga ttaaccgggt ccagttcgct ggaacgctgg gcaagcatgg
    16201 actgggtgat aaccagctcg ccgacgaggt taattaattg atcaaccttt tctaccgcta
    16261 cacggatgct ggtggattca ttgctgcgcg tcgttttttc ccgctccacg cggccggttg
    16321 gcgcttgttc ggctgccagt ttaagcactg gtggggtgga tatttttggc gagacttcta
    16381 ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca gagtaccgct gtgatgtcat
    16441 cttcggcgat gtcgcccggt aatattgccg agagcgaatc cgcccctttc accacgtcag
    16501 ttaacgttgt cagatgtccc agttcttctt ccagcaggtc gacttccccg gccttcaggc
    16561 gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg ctcatcttgc ggttcacttt
    16621 tggcaaccac acttaatcgg gtcactgcgg atggcgtttc gcctttcgct tctaatgcca
    16681 gttgacgcaa ggcctggcag atataatcga agctggcggc atccggctct tgcgactgtt
    16741 tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga ttgataatgt
    16801 cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc atcagatgcg
    16861 tggtttcctg caaaacgctg aagccaaaag ttcctgcccc tcctttgatc gagtgggcag
    16921 cccgaaagat ggcattcaat tgttcggcat ctggcgcttc cggctgcaaa accagcagat
    16981 gctgctccat gtcagccaac agttcgtccg cttcatcaaa aaatgtctga taaaaatcgc
    17041 ttatatccat gctcacgctg tcacctcggt tcggctgatg gcattgtggg aacactgacc
    17101 tgtggtgcaa cctcaggttt ttccagggcg cttactggct cattctggct ttcggcgttt
    17161 tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca gcaggctgat gcgacggttg
    17221 acggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc gacgacacgt
    17281 aacactttgc cgctatccaa ccctccgacc atcagttcgc ggcgggatgc attggcccga
    17341 tcggcagaaa gctcccagtt gctatatcct ttctcaccgc tggcgtaggg gaaatcatcg
    17401 gtatgacctg aaaggctaat acggttggga ataccgttca gtacaggcgc aatggcacgc
    17461 agaatgtcgc gcatataggg ttcgacatcg gcactgccgg ttctaaacat cgggcgattc
    17521 tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt gagatgggga
    17581 cgtaacgccc gcagtttcgg atcggactct atcaactggt cgagatcacc ccgcaatttc
    17641 cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg cttattcact
    17701 tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc actattagaa
    17761 atgagatcgc cgcccgtaac cgcagtcgcc agtggagtcc ggaagtactc cgcaatctga
    17821 atcagctctt ttgggctgga gatggagatc agccacatca ccagaaaaaa ggccatcatc
    17881 gcagtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc gtggcttttg
    17941 gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct tcctcggttg
    18001 tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagttca ataaacgacg
    18061 gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc ggtgcgtaac
    18121 cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg gtttcggcgc
    18181 ttttctgacg taaaacactc gctaatgggg aaataaatcc gtaagccaat aaaatgccga
    18241 ggaaagtccc caccatcgca tgtgcgataa gcgcacccag ctcggcggca ggacgatcgg
    18301 ctgaacctaa cgcgtgaacg acccccatta cagccgcgac aataccaaac gccggaagtg
    18361 agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg tgcgtctcaa
    18421 tctcttcatc catcagggct tcgatttcga aggtgttcat atgaccgctg ataatcagac
    18481 gcagataatc gacgataaaa tcaagcatga cgctatccgc gaggatgcgt gggtagctgg
    18541 cgaagatctc gctctcacgg ggattttcaa tatcacgttc cagcgaaaac atccccatct
    18601 gccgcgattt cgccatcaac cgataaagca gagccagcag atccatatac attgctttgg
    18661 tgtatttgga gcgacgaaac agcaacggca gcgccttcag cgtgccttta atcgctttgc
    18721 cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc agttcagcgg
    18781 gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa actgtaccga
    18841 gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt tgaccatgac
    18901 aggatgttca gtcgtcaggc gttaacgcgc gattggggca aaaaaaagca gcggtacgtc
    18961 gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt actctctgtt
    19021 catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt ctttttactg
    19081 cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg tgagcgtggg
    19141 taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca ctttcaacaa
    19201 accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct gcttgtgggc
    19261 actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta cacaaaccgg
    19321 ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt tgttcccagg
    19381 tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt ccgcgcagtt
    19441 ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc agcatctgca
    19501 aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccacgct tcctgaacaa
    19561 tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag gctgattaac
    19621 atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga gatcgtcaac
    19681 gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga agtgacaaac
    19741 cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg ctaacgttgt
    19801 cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct gaacaatcaa
    19861 acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca gcaactcgga
    19921 ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt caccgcaccc
    19981 cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt tgcgataagc
    20041 tgcaataagc agaaccacct ttttggttta atatgtcctt acaaatagaa atgggtcttt
    20101 acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac acgcacatac
    20161 aacggagggg ggctgcgatt ttcaataatg tgtgatgcag atcacacaaa acactcaatt
    20221 acttaacata aatgtataag tcatactttt gttttgggtg tatttcaatc tgttaaaaag
    20281 tttttcgcta cgctagcaag caaaaatgaa acaggaataa tcgaaatggg atgttgcgca
    20341 cagttaaaat aactcaccgt aaataatcat ctgctataaa taatcacttt catgcaatac
    20401 cagataagct atttttaaac agacacttac cgcacaacaa actaattaaa tacaaagaaa
    20461 ggcaacaatc aacttaatgt taatgaaatg ttttacatat attaaccact tgtttaatgc
    20521 ggagaatcag cacactattt tcttataatt acatttgaaa ttatatgatc accgggagtg
    20581 attgattaat tatctttact aataatcaga ctaatattta cctgcttgac cgagttggga
    20641 ttgcgtcgtt tctccattag gagtaaagct ttaatgtcac ctgaagttca cagaataagg
    20701 aacaggttat gagctatagc aatattcttg tcgctgttgc ggttacaccg gaaagtcagc
    20761 aactgctggc aaaagcggta tctatcgcca ggccagtaaa gggacacatc agtttaatta
    20821 ctctcgcttc cgacccggaa atgtacaatc aattagccgc gccgatgctt gaagatttac
    20881 gcagcgtgat gcaagaagaa acgcaaagct ttcttgataa gttaattcag gatgcggggt
    20941 atcccgttga caagacgttt attgcctacg gtgagttaag cgaacatatt ctggaagtat
    21001 gtcacaaaca tcatttcgat ttggtgattt gcggtaatca caatcacagt ttcttttcgc
    21061 gagcatcctg ctcggcgaaa agagttattg cctcaagtga ggtcgatgtg ctgttagttc
    21121 cacttacggg agattaaccg ctcctacgca agctttggaa aggtagcaac tttatcgcgc
    21181 tgctggcttt ccgcgcttcg cggaactatc tgctttaggt cgctaatgaa gcactcctgc
    21241 cagtggttaa tatcgttttt cacgataacg tccagcattt ctgcatgacg ggaaatacgt
    21301 tccgccagcg acatagtcaa tgcacgatcc agcgcagctg caacttcgtc acgatcgtag
    21361 gggttaacaa ttaacgccga cgttaactcg tttgccgctc ccgcaaattg cgaaagaaca
    21421 agaacgcccg ggttggctgg gtcctgagca gcaacatact cttttgctac caggttcatc
    21481 ccgtcacgca gtggcgtcac taagcccacg tcagagtagc ggaatatttt catcagtaat
    21541 ttacggtcaa aatgctgatt caaataataa agcggcgtcc agcctaattg cccgtattta
    21601 ccattaattc gtccagcttc attttcgagc tgatgacgaa tatcctgata ggcttgcaca
    21661 tcaccacgcg acgttggtgc aatctgggta taacgaattt taccatgatg ctgcggatat
    21721 ttttccagca acgcttcata ggcgagaaaa cgctctggca aacctttgga ataatccagc
    21781 cgttcgacag aaaagatatt ttgtacgttt ttcagttccg ctttaagttg cgccagtttt
    21841 ggcggcagtg gcccggcagc ctgtttggct atttctttcg gttcaatgcc gatcgggtag
    21901 acttctgttc gaaatgcttt gccccaggct gtatggcttt ttgcgctacg tgtcgtgacg
    21961 cgggtcaggt tagaaagaca atccaggaac gccagacgat cgttttctgt ctggaaaccc
    22021 agcaaatcat aatcacaaag ctgttcaagc aaggtgtcat atgtcggcag cgcgttgaag
    22081 atttccggtg tcgggaaagg aatatgcaga aagaaaccaa tgcgattatt cactccccgt
    22141 ttgcgtaatt catgcgcaaa tggcaacagg tgataatcgt ggatccagat aatgtcatcg
    22201 tcttgcaaca gcggcagtaa tttatctgcc agcaacgcat ttacgcgtag atagccgtcc
    22261 caggcaggac gctgaaattg caccagatcg agccgataat gaaaagcggg ccagagaacg
    22321 gcattggaga attggttgta gtattcgtca aggtcctgtt cgctgaggtt aaaagaggcc
    22381 cacgtaatgt tacctttttt cacctttttt agcggctgat cctcattccc tgtttcacca
    22441 ctccagccaa accacagtcc gcctgcggct ttcagtgccc ccagtatgcc aacggcaagg
    22501 ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc ggttagatac tacgactaaa
    22561 cgactcatag tcatcactcc tgttattttc tcttttttgt tgtaatgcgg tggttatcat
    22621 ttcaagccag ctccagacat ccggcacacc cgccagtcgc catgatgcct gagttgcacc
    22681 tgtgccaatt tttactgaca ttccgcccag tcggttaacg actgcgaagc cagattcatc
    22741 ggttaaatca tcgcccagaa atacgggcgt tcgcccgata aagggagctt cctgcataaa
    22801 agctgcaatt gcctcacctt tactggtacc tctcggtttg atctcgacaa cacactttcc
    22861 ctgctgtaac gccatttgtg gccagatctg agtaatacgt tgcgctaatg tcattaatgc
    22921 gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa gccatccctt tcgcctccag
    22981 atccgcgccg ggatactgag cgatgactgt atgcagttgc acgctaatat cacgcgcaat
    23041 cgcatccggc agatgaacga tatgtgtttt accattgatg tcacggcgct ccgccccatg
    23101 cacgcctgct aacgggaagc gataaggttt tgccagtgcg tcaagctcca ccattgagcg
    23161 ccctgatatc aatgccaatg caccatcact tgcggttgcc agtagctgta gtccttgcag
    23221 aatattgtca ggcacgacga cctgatcggg atgcggtttg atttccgcca gcgttccatc
    23281 aagatcaaaa aaccaggcat atttcgcgga tagttcaggg gtttcggtta acggttctgt
    23341 cacccggctc tcctccttct cttttcttat tgttttgcgc agaacgcgct ttacagacat
    23401 gtaagtatag acagtgtgac gggggtcgcc atttgcaatg aagaacagcc aaccgttgtg
    23461 gcggctggct gtggtgggaa aaaacgttaa attgttgtgg aaaaaagcat cagacagtgc
    23521 gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc cagcaggatt aagccgcgaa
    23581 ccacgtactg cgcgaaagga gaaatattaa gcaggttcat ggcgttttcc acggtgccta
    23641 aaattaagat acccgccacc acatatgaga tttttccgat gccacctttc agagaaacgc
    23701 cacctaaaac gcaggcggag ataacaataa gctcataacc aatcgacgtc attggctgcc
    23761 cactagtcat acgtgaagcc agaataattc cggctatcgc agataccagg ccagagagaa
    23821 caaagataat aattttggtg cgaacaaccg gtacacccgc cagacgcgca gcctcttcgt
    23881 tcccgccaat cgccagggtg ttacgaccaa aggtggtttt attcagcaac aaaccaaaga
    23941 taatcagaca cgcgacggtg agccagattg gcgcaggcag accgaaccag ttagcgtaac
    24001 caagggcaaa gaagctttca tcttcgatac cgaccgcttt accgtctgaa atgatgtacg
    24061 ccagacctcg aacaatctgc atcgttgcca gtgttgtgat cagagcattt attttcagtt
    24121 tggcgataac aaagccattg accaggccac agagaatgcc aagcagcaac cccgctgcca
    24181 cgccaatcca caggctttca gtcaggttga taaccaccgc cgtggtgaca cccgcacagg
    24241 caattacgga ggcgacagaa aggtcaaagt caccggaagc aaggcagaac aacatgccac
    24301 acgccaccat ccccgacatg gaaattgcca ggcccaaccc tttcatatta atgaaggtgg
    24361 caaaatttgg gacaaaaatg gcacaggcga taaagagcac cgcaaacacc accagcatgc
    24421 cgtactgatc ccagatacgc ccgaagctga atgacgactt aggtgcgcca gaccccgatg
    24481 tagaaacaga agacatcata ctctccttac tcaggcaact gcctggctga ctttaggcat
    24541 cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt aacaattcac cggcgatttc
    24601 accttcccgc atcaccacaa tccggtcggc aacgccgagg acttcaggta agtcgctgga
    24661 ggcaaacagc accgccacac cctgcgccgc cagcgcataa atcacgttgt aaatttcatg
    24721 cttagcacca acatcaatgc cgcgcgtagg ttcatccagc aaaatgacct tcatctcttc
    24781 cgataaccag cggcccagaa tggctttttg ctgatttccg cctgagagat tcatgatcag
    24841 ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg tggtgatcgg cattgttttc
    24901 ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt ttacgtctgg cactgatgtt
    24961 gatattgtcg cgaacggagt gcacgggaat aatgccttcc gctttgcgat cttccgggca
    25021 gagcatcatg cctgcggcaa tggcgtggct cggtttacga atatcgatcg gctgttggtc
    25081 gatataaacc tgaccggcgg tgatttgcgt cccgccaaac aagcctttca ttaattcgct
    25141 acgccccgcc cctaccagac caaacagccc aacaatttca ccactgcgaa ccgccagact
    25201 tattggcgta cgcacgcctg gtgctttcac agcatcaaga cgcaggcgct cctcgccata
    25261 actacgaggt tgccagccgt agatatcgcc aatgtcgcgc ccgaccatcg cctgcaccag
    25321 cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg acataacgtc catctttaaa
    25381 gacagtgatg gcatcgctga gggcaaatat ttcttccata cggtgagaaa cgtataagat
    25441 cacccgcccc tcttttcgca gttcacgaat aacgcggaaa agattgtcga tttcgcgggc
    25501 agaaagggag ctggttggct catcaaaggc gataatttta gcgttacgcg ccagcgcctt
    25561 ggcgatttca accatctgcc actgaccaat ggagagatat ttcagcggcg tgtccgggtc
    25621 aatatccata ccaagatgtt taagttgtaa acccgcctca taattcagca atgagcgatt
    25681 cacaatgccg cctttatgcg gcagctggcc gagatagatg ttttccgcga cggtcatttc
    25741 cggcacgaga tgtagttcct ggtaaataat cgccacaccc gcgttaagtg ctgcggtcgt
    25801 gtcggaaaag gacatttcct gcccattaat cactacagaa cccgtggttg gcgcatagtt
    25861 gccgctgagg atttttaaga gagttgattt tcctgcgcca ttttcaccca tcaacgcatg
    25921 aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt
    25981 tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat
    26041 cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt
    26101 accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac
    26161 gtcggtaact tcggtaaatt ttggcggttc aacgtctttt gctacccagt tgtaaagcat
    26221 ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt
    26281 tgcctgtgct ttagacagtt cgctcaccgc atccacaccg ttaatgccaa tgccgatgat
    26341 atcggccgct ttaaagccct gaccttccgt cgcgcgtacg ccgcccagca cggtgctgtc
    26401 gttcataccg acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc
    26461 agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc
    26521 cgggaatccg gccgctttca gcgcatccat agatcccgta gtacggcggc gggcggtatc
    26581 cagttcgttg gcggtaatcg ccatcaccgc gctttctttg acatcccagc cacgtttctg
    26641 catctcttta tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac
    26701 cagcggaacg gtatccattg gcttaccttt ggcgttaaca aactggtcat ccacggcaat
    26761 gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt tggggtccgg
    26821 agtacaaata acgaaacctt ttgcgccact ggcagccagg ctgtcgatcg cgttcaatgt
    26881 tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt
    26941 atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc
    27001 gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc
    27061 cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc
    27121 aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt taactaaaag
    27181 ataagtgact gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat
    27241 cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac ataagggaaa
    27301 gccaatttgt cagacaaatt gtcgaatgca cagcagatta atccataaga ttagcctgga
    27361 aatccttgtt gtctttggta cccatgcggg atgtcttctt tttaaccagt caataggccg
    27421 cattacctgg cgttgagttt ttgaaatggt gtaataaccg caactcaaag atgtggaaaa
    27481 tgcacgtcat tcatttcgcc attaattatc actgtactca ttaattaaca gaacacgtat
    27541 aacgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa
    27601 ttcaaattca ttgattaata tcaacaagat acaaaaagcg ctatcattaa aattcattgc
    27661 agttacattg atttcatcaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt
    27721 tcttataccc gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat
    27781 cacatttcgg accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt
    27841 atcttgcacc tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct
    27901 tctcaaactc aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct
    27961 gagtaactgg tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc
    28021 acagagtaat gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac
    28081 ccccatcgtt aaagccattg atgttcccgg tgaaaaactg aactctctgg aagaactgtt
    28141 ccaaaaatcg atggaagaat acgagcaacg ttctagtacg ttggcacagt tagccgatga
    28201 agcgaaagaa ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca
    28261 gcagcatgat ggtctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc
    28321 gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca
    28381 ttgatcatca tcggcgctaa tgcattgcgc cgatgaaggt tttgagaaac cgctgcctca
    28441 tctgtttgaa gcagcggttt ttttaatggg attcaccctg tggggtaaac ttgagttcaa
    28501 taagcgcgat ggctttttgg attgcccgca tggtgacagg gtctgcggcg gcgggatggt
    28561 tagtaaagtc gatattcttc agctgactgg acattttttc acgaacttca acgggcgcga
    28621 ttacatcgag aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa
    28681 ccagttcctg atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa
    28741 gccgttatag tagcggcttc acatcttcag cgatagtcac atccaccgtc atcaggacac
    28801 aaaaaaacct gccggagcag gttttttatt atcggaacat attgcctggc ggtacgtctt
    28861 tgaacgtctt gcaatagtta ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg
    28921 cactccgacc atttgttata caggtgttat tctctttgcg cttataatat gacaaccatc
    28981 acaaaaatca atctttatgt gatacaaatc acatagatac ccctttaatg ttataaaaat
    29041 gataatcaaa aaacagcccc cctatttctg acacctacag atggcaagaa atagcgcctg
    29101 ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt
    29161 actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata acgctggcgg
    29221 ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca ggtattgtac
    29281 ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat gtcagtcgcg
    29341 ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcggtcccc tttggtcagc
    29401 cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa ttcactttgt
    29461 catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa tcagcgaggg
    29521 gatcttcgct gattaaagaa atagcggtaa tgcgttttat tcccattcgt attttt
//
LOCUS       DAZUFB010000054        29237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZUFB010000054 DAZUFB010000000
VERSION     DAZUFB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.054"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            416..1282
                     /gene="folD"
                     /locus_tag="ACHY3M_002694"
     CDS             416..1282
                     /gene="folD"
                     /locus_tag="ACHY3M_002694"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFS4317586.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1284..1496
                     /gene="ybcJ"
                     /locus_tag="ACHY3M_002695"
     CDS             1284..1496
                     /gene="ybcJ"
                     /locus_tag="ACHY3M_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFS4317587.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1604..2125
                     /gene="ybcI"
                     /locus_tag="ACHY3M_002696"
     CDS             1604..2125
                     /gene="ybcI"
                     /locus_tag="ACHY3M_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4317588.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2161..3546)
                     /gene="cysS"
                     /locus_tag="ACHY3M_002697"
     CDS             complement(2161..3546)
                     /gene="cysS"
                     /locus_tag="ACHY3M_002697"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4317589.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3720..4214
                     /gene="ppiB"
                     /locus_tag="ACHY3M_002698"
     CDS             3720..4214
                     /gene="ppiB"
                     /locus_tag="ACHY3M_002698"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFS4317590.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4217..4939
                     /gene="lpxH"
                     /locus_tag="ACHY3M_002699"
     CDS             4217..4939
                     /gene="lpxH"
                     /locus_tag="ACHY3M_002699"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFS4317591.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESSMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5057..5566
                     /gene="purE"
                     /locus_tag="ACHY3M_002700"
     CDS             5057..5566
                     /gene="purE"
                     /locus_tag="ACHY3M_002700"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4317592.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5563..6630
                     /gene="purK"
                     /locus_tag="ACHY3M_002701"
     CDS             5563..6630
                     /gene="purK"
                     /locus_tag="ACHY3M_002701"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4317593.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPRLPPEYASGVMWAQSKFS"
     gene            complement(6767..7660)
                     /gene="ybcF"
                     /locus_tag="ACHY3M_002702"
     CDS             complement(6767..7660)
                     /gene="ybcF"
                     /locus_tag="ACHY3M_002702"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4317594.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7657..8472)
                     /gene="ylbF"
                     /locus_tag="ACHY3M_002703"
     CDS             complement(7657..8472)
                     /gene="ylbF"
                     /locus_tag="ACHY3M_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS4317595.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8483..9742)
                     /gene="ylbE"
                     /locus_tag="ACHY3M_002704"
     CDS             complement(8483..9742)
                     /gene="ylbE"
                     /locus_tag="ACHY3M_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS4317596.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9752..11419)
                     /gene="fdrA"
                     /locus_tag="ACHY3M_002705"
     CDS             complement(9752..11419)
                     /gene="fdrA"
                     /locus_tag="ACHY3M_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS4317597.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11736..12785
                     /gene="allD"
                     /locus_tag="ACHY3M_002706"
     CDS             11736..12785
                     /gene="allD"
                     /locus_tag="ACHY3M_002706"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFS4317598.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12807..14042
                     /gene="allC"
                     /locus_tag="ACHY3M_002707"
     CDS             12807..14042
                     /gene="allC"
                     /locus_tag="ACHY3M_002707"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFS4317599.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14053..14838
                     /gene="allE"
                     /locus_tag="ACHY3M_002708"
     CDS             14053..14838
                     /gene="allE"
                     /locus_tag="ACHY3M_002708"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFS4317600.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14967..16112)
                     /gene="glxK"
                     /locus_tag="ACHY3M_002709"
     CDS             complement(14967..16112)
                     /gene="glxK"
                     /locus_tag="ACHY3M_002709"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFS4317601.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16134..17435)
                     /gene="ybbY"
                     /locus_tag="ACHY3M_002710"
     CDS             complement(16134..17435)
                     /gene="ybbY"
                     /locus_tag="ACHY3M_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFS4317602.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17492..18853)
                     /gene="allB"
                     /locus_tag="ACHY3M_002711"
     CDS             complement(17492..18853)
                     /gene="allB"
                     /locus_tag="ACHY3M_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFS4317603.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18913..20367)
                     /gene="ybbW"
                     /locus_tag="ACHY3M_002712"
     CDS             complement(18913..20367)
                     /gene="ybbW"
                     /locus_tag="ACHY3M_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFS4317604.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20536..21414)
                     /gene="glxR"
                     /locus_tag="ACHY3M_002713"
     CDS             complement(20536..21414)
                     /gene="glxR"
                     /locus_tag="ACHY3M_002713"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS4317605.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21514..22290)
                     /gene="hyi"
                     /locus_tag="ACHY3M_002714"
     CDS             complement(21514..22290)
                     /gene="hyi"
                     /locus_tag="ACHY3M_002714"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFS4317606.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22303..24084)
                     /gene="gcl"
                     /locus_tag="ACHY3M_002715"
     CDS             complement(22303..24084)
                     /gene="gcl"
                     /locus_tag="ACHY3M_002715"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFS4317607.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24174..24989)
                     /gene="allR"
                     /locus_tag="ACHY3M_002716"
     CDS             complement(24174..24989)
                     /gene="allR"
                     /locus_tag="ACHY3M_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFS4317608.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25067..25549)
                     /gene="allA"
                     /locus_tag="ACHY3M_002717"
     CDS             complement(25067..25549)
                     /gene="allA"
                     /locus_tag="ACHY3M_002717"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFS4317609.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25779..26705
                     /gene="allS"
                     /locus_tag="ACHY3M_002718"
     CDS             25779..26705
                     /gene="allS"
                     /locus_tag="ACHY3M_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFS4317610.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26774..27868
                     /gene="mnmH"
                     /locus_tag="ACHY3M_002719"
     CDS             26774..27868
                     /gene="mnmH"
                     /locus_tag="ACHY3M_002719"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFS4317611.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27984..28349
                     /gene="ylbG"
                     /locus_tag="ACHY3M_002720"
                     /pseudo
     CDS             27984..28349
                     /gene="ylbG"
                     /locus_tag="ACHY3M_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28870..29043)
                     /locus_tag="ACHY3M_002721"
     CDS             complement(28870..29043)
                     /locus_tag="ACHY3M_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317612.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
     gene            complement(29100..>29237)
                     /locus_tag="ACHY3M_002722"
     CDS             complement(29100..>29237)
                     /locus_tag="ACHY3M_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317613.1"
                     /translation="HYINYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIF
                     P"
BASE COUNT         7246 a         7880 c         7230 g         6881 t
ORIGIN      
        1 actgatagta gtaaaattcc tttttatcct ctaagaatgt cttaattgaa aatatgcact
       61 ctattctaaa aaatagagag ccccgttaga tgaatacttt cgcgcaaaat atattcaaca
      121 caaatataga cctgaagcgg taaattacca ggctgaaaat tctttttata ttgtcaggta
      181 tttcttaaat tatcttaatc cttaaacaag gaaataaatc agttccagat ttacaacgcc
      241 atcatggacg aaaaatgaag ctttcaatct cagcgacctc agcgacggtg cgcctcacct
      301 tcgcaagagg tcgcttcacg cgataaatct gaaacgaaac ctgacagcgc gccccgcttc
      361 tgacaaaata ggcgcatccc cttcgaccta cgtaacagat ggaatcctct ctctgatggc
      421 agcaaagatt attgacggta aaacgattgc gcagcaggtg cgctctgaag ttgctcaaaa
      481 agttcaggcg cgtattgcag ccggactgcg ggcaccagga ctggccgttg tgctggtggg
      541 tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc aaggcttgtg aagaagtcgg
      601 gttcgtctcc cgctcttatg acctcccgga aaccaccagc gaagcggagc tgttggagct
      661 tatcgatgcg ctgaatgccg acaacaccat cgatggcatt ctggttcaac tgccgttacc
      721 ggcgggtatt gataacgtca aagtgctgga acgtattcat ccggacaaag acgtggacgg
      781 tttccatcct tataacgtcg gtcgtctgtg ccagcgcgcg ccgcgtctgc gtccctgcac
      841 cccgcgcggt atcgtcacgc tgcttgagcg ttacaacatt gataccttcg gcctcaacgc
      901 cgtggtgatt ggcgcatcga atatcgtcgg tcgtccaatg agcatggaac tgttgctcgc
      961 aggttgcacc actaccgtga ctcaccgttt cactaaaaat ctgcgtcacc acgtagaaaa
     1021 tgccgatctg ttgatcgttg ccgtcggcaa gccgggcttt attcctggtg actggatcaa
     1081 agaaggcgca attgtgattg atgtcggcat caaccgtctg gaaaatggca aagttgtggg
     1141 cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac attacgcctg ttccgggcgg
     1201 cgttggcccg atgacggttg ccacgctgat tgaaaacacg ctacaggcgt gcgttgaata
     1261 tcatgatcca caggatgagt aacatggcga cattttcttt aggtaaacat ccgcacgttg
     1321 agctgtgcga cttgctgaaa ctggaaggct ggagcgaaag cggcgcgcag gcgaaaatcg
     1381 cgattgccga aggccaggtg aaagtcgacg gtgcggttga aacgcgcaaa cgctgcaaaa
     1441 tcgtcgccgg tcagacagtg agttttgcag gtcacagcgt acaggttgtt gcctgatccc
     1501 tttcccggcc tgtatcaaca ggccggttct cttttcagac ttttgccctc tacgccccgt
     1561 tcatccatgc tgatagcatc gacattttgc cgaagcgacc atcatgccaa ccgttattac
     1621 gcacgccgcc gttcccctat gcattggttt agggttgggt tcgaaagtca tccctccgcg
     1681 tctgttattt gccggaatca tcctggcgat gctgcccgac gccgacgtat tgtcgtttaa
     1741 atttggcgtt gcttacggca atgtttttgg tcatcgcgag tttacccatt cgctggtgtt
     1801 tgcgtttgtt gtcccgctgt tatgtgtgct gattggacgc cgatggttca gggctgggct
     1861 gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttgc tggattcggt
     1921 aaccactggc ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc
     1981 tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cgccgtacgg
     2041 gcatcaggtg attatttctg aattgatgtg ggtgtggttg ccggggatgg tgcttatggg
     2101 aatgttgtgg tggcgcaggc gataaccgga tgcgaaaact cgcatccggc aacagcgcaa
     2161 ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt
     2221 aagacggtca cgcgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg
     2281 ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg caccgctttg
     2341 caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg
     2401 agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc
     2461 catatcaaac agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat
     2521 aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag
     2581 cgcaatgtag aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt
     2641 cagttggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta
     2701 tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc
     2761 aaccatcacc atccccgagt gcatccagta gttcacatac tggccatcat gggcacaggt
     2821 ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat
     2881 atcaaagtgg ttacccagct gcttgcagtt cattgccgaa cattcaatgt gccagcctgg
     2941 acggcccgcg ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag
     3001 aacgaagtcc atcgggttgc gtttgtcgtc gaccacgtca acgcgcgcac ctgcctgcag
     3061 ctggtcgaga tcctgacgcg acagcacgcc atatgtcgga tcggtcggga cgtcgaacat
     3121 cacgtcgccg ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc
     3181 aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa
     3241 agcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc
     3301 gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata
     3361 cttcagttta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt
     3421 acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacat acatgccgac
     3481 ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt
     3541 tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat
     3601 ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac
     3661 caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa
     3721 tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac
     3781 ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt
     3841 tccaccgtgt tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac
     3901 aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggtctgaaa aatacccgtg
     3961 gtacgctggc aatggcccgt actcaggctc cgcactctgc aactgcacag ttcttcatca
     4021 acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact
     4081 gcgtgtttgc tgaagtggtt gaaggcatgg acgtggtaga caaaatcaaa ggtgttgcaa
     4141 ccggtcgtag cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga
     4201 ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga
     4261 accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc
     4321 gctgtatatt cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact
     4381 ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt
     4441 cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcagcatgac
     4501 attattgccg gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg
     4561 cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc
     4621 ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg
     4681 aatgcgagcg aacagtaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa
     4741 ccaaaacgcg gtagtcagtg cgatggaaaa acatcaggtg caatggctga tccacggaca
     4801 tacccatcgc ccggcggtac atgaacttat cgccaaccag caacctgctt ttcgcgtggt
     4861 actcggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggcggatg acgttgagct
     4921 gattcatttt ccgttttaaa aaacccgcaa ctttgctgat ttcacagcca cgcaaccgtt
     4981 ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac
     5041 aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg
     5101 gtccaaaagc gactgggcta ccatgcagtt cgccgccgaa atcttcgaaa tcctgaatgt
     5161 cccgcaccac gttgaagtgg tttctgctca ccgcaccccc gataagctgt tcagcttcgc
     5221 cgaaagcgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca
     5281 cctgcctggt atgattgccg ccaaaacgct ggtgccagtg ctgggcgtgc cagtacagag
     5341 cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc
     5401 ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcgttac tggcagcaca
     5461 aattcttgcg actcatgata aagaactgca ccagcgtctg aatgactggc gcaaagccca
     5521 gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc
     5581 ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag gtgaaccgtt aggcattgct
     5641 gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt
     5701 accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg
     5761 gcctttgtga accgcgatgt gttcccgatt attgccgacc gtctgactca gaagcagctt
     5821 ttcgataagc tccacctgcc gaccgcaccg tggcagttac ttgccgatcg cagcgagtgg
     5881 cctgcggtgt ttgagcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat
     5941 gacggtcgcg gtcaatggcg tttacgtgcc aatgaaaccg aacagttacc ggcagagtgt
     6001 tacggcgaat gtattgtcga gcagggcatt aacttctctg gtgaagtgtc gctggttggc
     6061 gcacgcggat ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac
     6121 ggtattttgc gcaccagcgt cgcttttccg caggccaatg cgcagcagca agcgcaagcc
     6181 gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag
     6241 tgttttgtca ccccgcaagg tctgctgatc aacgaactgg caccgcgtgt gcataacagc
     6301 ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt
     6361 accgatctgc cgttaccgca accggtagtg aatagtccgt cggtgatgat caacctgatt
     6421 ggtagcgatg tgaattatga ctggctgaaa ctgccgctgg tgcatctgca ctggtacgac
     6481 aaagaagtcc gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg
     6541 cgtctgaccg cgacgctgga agccttgatc ccgcggctgc cgccggagta tgccagcggc
     6601 gtgatgtggg cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc
     6661 gtaaacgcct tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca
     6721 agcgtcgcat caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac
     6781 aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg
     6841 gtttaccacg gcttctgaca taaccactta ccgccgttac cttcggcccc atcgaaccat
     6901 cggctttggc aaatggcgct aactcatccg gtgtggcatg gcgaatggcg cgttgctgcg
     6961 gcgttcccca gttttcataa accgcgtcag catcggtgag gatcaccagt ccatcggcat
     7021 taatctgctc ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg
     7081 ccccatcttc cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt
     7141 tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca
     7201 cccggcgcaa atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct
     7261 tttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat
     7321 catcaggcga aacctcaatg cgcgtcagca ccgtcgtgac aggcggcatc tgcggctgtg
     7381 cgctcaaact ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagcacat
     7441 ccagcggata cggatctacc tctttccacg ccagattctg caatgccagc agccccacct
     7501 gcggcccatt gccgtgaaca atcgccagcc gataagaacg ggccaggcgc gccagcgcgg
     7561 gtacagcact ggcgatattg cgatattgat tttctgccgt cagcgcctca ccgcgctgga
     7621 gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg
     7681 ttggccaagc cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag
     7741 cgaatcaatc gcaacagcgg tacgcttcgg gcaactcaga gcatggacaa agtgcaggag
     7801 tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt
     7861 aacgagttga agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc
     7921 gccataatac caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa
     7981 tccggggcct ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg
     8041 aaaacagtga cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag
     8101 tggtccgaaa agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg
     8161 atgcgaaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg
     8221 cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca
     8281 taacgcatcg aattgcgcgc ggcgaagcac ccatcctccg gggccgaaac cactaccctg
     8341 acgatgcagc gttaacagtt cgccgctttc cgtcatcagg ttaatcgccc gtcgccacac
     8401 tcccgctaac cgccaggact ggcgataatt cggtgcacta cggctggcaa gcagaggatg
     8461 gatgatcgtc attgcgcgtt cctcaaccaa tccccatgct ttcagccagt gcttccagcg
     8521 cctgttcaaa acacgccagc ggtgcccgca cagtgcctgc gccaatctgc ccgatccccg
     8581 cctctttatg ggcgataccg gtattgatga gtggcgtaat gccggtttct accacgcgac
     8641 gaatatccag ccccaggcac gcgccctgga aatcccagcc ggggatctgc aactgcatat
     8701 tgcgctcaag gtaaatttcc gccatctctt cagataccgc tctggccgct tccatgccac
     8761 ccgcaccgac aaagcgcgtt acgccaggcg ctgcgatcat tgccgcacct ccgataccaa
     8821 aggtttcggt aatcgcgcta tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg
     8881 tgaaaaacag accttgcgga gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc
     8941 ttacccgaat cccgaacata ttgccgttgc gggtcattgc cgtgacgatg ctgcctgcgc
     9001 ggatcatcgc gccagcatcc atcgccgcct tgcagtaagc catcgcgagg ttgaggaaga
     9061 actgatcggt cacgctgagg aaatccatca cttcggcgat gtgctgttta tcatgatcga
     9121 ggcgagcaat ttgtggggcc agcgcacgca ttaacagtgc agaggaagca atattgcgtt
     9181 gatggaactc atcgcccatc gtaatgccct gcgccatcat cgccgtgaga tcgataccgc
     9241 gctccatgcg ccccagcgcc gcgcttaata ctggcattaa cacatcgcgc atccagcgat
     9301 ggcgagtcag gacatcttcg ccgtaagcgc caaaacgcat cactttgccg ataccttcgt
     9361 tgaggttgca gtacgcccgg ttgccgtcgg tcacgttctc gaccaccagc atcggcatac
     9421 tggcagaagt aataccgccc attggaccga cggcattcac atggtgacaa ggaatgaagt
     9481 tcacttcccc ctgctccagt attgccagcg cctgcgcttc atctttcgcc cagccttcga
     9541 acagacatgc gcccacgcac gcccctttca tgggtccggt catctcctgc cagcgcattg
     9601 gcggcccggc gtgaagcagt gttttgccct cgtttagttc gctgataagt gaagaagccg
     9661 gttgcacatc cagccagtgt ggacgagcac gacgaatttg ttcgataacc gcagcattgg
     9721 cttgcgccac tgatgtaaac atgggaaccc cttattgcaa acgttctaat aaacgagcca
     9781 gttttttatt gccgccggcg actggcgacc attggtaatg cacaaccggt ttgctggcgc
     9841 tttgtagctc cagcgcaaag ctgcgtaatc cgatgttaat cacggcgacg ttttccagta
     9901 atgacggtgt gtgttgctgt gtggcagacg agagcggatg aattaacgct gccgccagca
     9961 aggtggcttc cggtagcgaa ctcactaccg cgatccccgc atcttccagc gtggcgattt
    10021 gctgcgagcg gcattgcggg tcacgttcgg tacctgtcac cgtggcaatg gcgtacagcg
    10081 gttggctgtc tgaacgcgcg gcacaggctt tttgccaggc gctcaccagc gaggcagcag
    10141 gatcggcggt cgcaccgaag ccaatcacga catcaagcag caacacgcgc acttgcggtt
    10201 tagcgccgag atcggcaatt aactggttgc gtaaggctgg gtcgatcatc ggatggggac
    10261 gcccgacggt gtagaaatca tcgcccagat caatgatctg gtgaccatcg gcgtccagca
    10321 tcatgccgtg atgatgggta tcgtcggctt ccacgccaag gtgcccggca agtaatcccg
    10381 ccgcttcggc agcaagcgta ccgccggtat acaaaccgca aataaatccg ctgctgacag
    10441 gcgctattgc gttacgtcgc gccgtgacgc gtgaaagcag gcaagccagg cgtgcggcct
    10501 catccagcga ggaggcaaac cagacattct cgtcgcgggc caccgccggg gtataaccta
    10561 aaaacagcgc caccgtcggt ttgccggttg ctttcatggc attaacaatt ttcagacgca
    10621 cagcttcggc aggtggtttt gaaacaaatg ccagcacttc gcttttctcg tctgcactga
    10681 gcatttccag cgctgttagc gcactgatgc cgcccacttc acggctgagg tcgcgcccgc
    10741 caaggccaat cgcgtgagta attccctccc ctgccagcgc aatctgcgaa cacagctcct
    10801 gaatcccggt accggaagcg ccaatgacgc caatattgcc ttctggcatc acgttagcaa
    10861 aagccagcgg tgtgccggca atcatcgacg taccgcagtc cggccccatc accagcaagc
    10921 ctttttcccg cgcgcgggtt ttaagttgga tttcatcttc cagcgtgacg ttatcggaga
    10981 acatcatcac gttgaggttg cgatccagcg cctggtttgt cagctccgcc gcatactcgc
    11041 cagccactga aatcagcgcc aaattggcat cgggtaattt ctgacaggcg ctgtcccagc
    11101 gacgcacctg cgtcaacgcc tggctgctgc ctgacccctg cgccagttgt tttagcgcct
    11161 cttcaagctg ctgcataatc gcctgcgcga tccccgcatc cgccgcttcg ctacgaattg
    11221 ccacgcaaat atcgttcggc gtggcgttat taaaatcgtc atgccagaaa cctgtggtat
    11281 ctaataacgc tttattggcg ggcgtaccca tcattacgga aacatcatca acattttctg
    11341 attcgctgag ttttcgtgaa ataatcatta aactgaccga atcctgaaaa cacccttttt
    11401 taataaaggc gtggatcata acaactcctt aaaaattgca cccatggcaa ttgcacgagc
    11461 agtgttaatg tggcgattac ggtatcgatg acaacgagta aataaggtga tgaatatcac
    11521 acagggatta ttaacaaaag aattcaaata accgccaggc tattacgcat tattttttaa
    11581 taagcgtggt tgttttaaaa atcaggttaa aagcgcgaag attatcaaat aaattctaac
    11641 tatatttttt tgcctgtctg gatcacataa tccagatatt ttccctgtat gttaattgca
    11701 gtcatgcttc acaccgtcgt taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc
    11761 accagctaat tgagaataaa ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg
    11821 tggctgaagt attggtttac gccgatgcca gagggatcca ctctcatggc gcggtgcgcg
    11881 tggaatacta cgcggaacgc atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc
    11941 ttgaggaaac cgggccgtgc tcggcaattt tacatgccga caatgccgcc ggacaggtcg
    12001 cggcgaaaat gggtatggaa catgccatca aaaccgccca gcaaaatggc gttgcggtgg
    12061 tcggtatcag ccggatgggt cacagcggcg caatctctta ttttgtgcag caggcagccc
    12121 gcgccggatt aattggtatt tcgatgtgcc agtccgatcc aatggtggtg ccgtttggcg
    12181 gcgcggaaat ttactacggt actaacccac tggcctttgc cgcgccggga gaaggcgacg
    12241 agatccttac ctttgatatg gcgactaccg tacaggcatg gggaaaagtg ctcgacgccc
    12301 gctcgcgtaa tatgtctatc ccggatacct gggcggtcga taaaaacggt gcaccaacaa
    12361 ccgatccgtt cgcggtacat gctctgctcc ccgccgccgg gccaaaaggg tatggcctga
    12421 tgatgatgat tgacgtcctc tcaggcgtct tactcggctt accgttcggg cgacaggtta
    12481 gttcgatgta tgacgattta cacgccgggc gtaatttggg gcaattacat atcgttatta
    12541 acccgaactt tttcttctcc agcaaattat ttcgtcaaca tcttagccag accatgcgcg
    12601 aattaaatgc cattaccccc gcgcccggtt ttaatcaggt ttattatccc ggacaggatc
    12661 aggatattaa acaacgcaaa gccgccgtcg aaggcatcga aattgttgat gatatttacc
    12721 agtatttaat ttccgacgcg ctttataaca cgtcatacga aacgaaaaat ccctttgcgc
    12781 aataattatt gagacaggac tttcttatga ttacacattt tcgtcaagcc atagaagaaa
    12841 cgctgccctg gctttcctct tttggcgctg acccaacggg tggtatgacc cgtttacttt
    12901 attcgccgga atggctggaa actcagcagc aatttaaaaa aagaatggca gcaagcgggc
    12961 tggaaacacg tttcgatgaa gtggggaatt tatatggtcg cctgagtggc accgaatatc
    13021 cacaagaagt ggttctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg
    13081 acgggcaatt cggcgcgctg gcggcgtggc tggcaattga ctggctgaaa acgcaatacg
    13141 gcgcgccgct acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc
    13201 cgtatgtctt ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga
    13261 atatctgtga tgccaaagga aatagttttg tcgatgcgat gaaggcttgc ggatttactc
    13321 ttccgaacgc cccactaact ccgcgtcagg atattaaagc ctttgttgaa ctgcatattg
    13381 aacagggctg tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg
    13441 ggcagcgtcg ttatacggta acgctgaacg gcgaatcaaa ccatgcaggc accacgccga
    13501 tgggttatcg tcgtgataca gtttacgctt tcagtcgcat ttgccatcag tcggtcgaaa
    13561 aagcgaaaag gatgggcgat ccgctggttc tgacctttgg caaagtagag ccgcgcccga
    13621 atacggtaaa tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt cataccgacg
    13681 ctgccgtgct gcgcgatttc acccaacagt tagaaaacga catgcgggcg atttgcgatg
    13741 aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata
    13801 aggagctggt cgccaccctg acagaattgt gtgaaagaga aaaactgaat taccgggtga
    13861 tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga
    13921 ttttcattcc cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattaccg
    13981 accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcagaaat
    14041 aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgatga tttactggct
    14101 aaccgtgcga ttgttaaaca cggtaatttc gcactgttaa ccccggatgg tctggtaaaa
    14161 aatattattc cgggctttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt
    14221 gcctcttttg ttgattatct ggtcacactg catcaaaacg gtggcaacca acagggcttc
    14281 ggtggcgaag gcattgaaac gttcctgtat gtgatctctg gaaatatcac tgccaaagcc
    14341 gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccacc aggctcctta
    14401 atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa gcgccgctat
    14461 gttccggtag aaggccatgc accgtggctg gtttctggta atgccagcga actggaacgc
    14521 attcactatg aaggtatgga tgatgtgatc ctgctggatt tcctgcccaa agagttaggt
    14581 tttgatatga acatgcatat cctctctttt gcgccaggtg ccagccacgg ttatatcgaa
    14641 acacacgttc aggaacatgg tgcctatatt ctttccggtc agggggttta taacctcgac
    14701 aataactgga tcccggtgaa aaaaggcgat tacatcttta tgggcgctta ttctttacag
    14761 gctggttatg gcgtagggcg tggtgaagcg ttcagctata tttactcgaa agattgcaac
    14821 cgcgacgtag agatttaagt cataccactc gcctgattat ttttagctat gttggatttt
    14881 gccacggttt ataccgtggc tttttttgta gtaaattcag gtaaaaaaag atgcgaaata
    14941 aatcgcatcc gctttaaagg taagggtcag tttttaattc cctgacctat tttaatggcg
    15001 caggcaatat ttcgcgcgct gttgaagaga ttggtttcac cgctggcgag cacttcggct
    15061 aaaggtgcca gacgaggcaa aatgctgaat accgcgtcaa tgccgtactg gtgcaccact
    15121 tccacgccat cacccaatac gccagcaatc ccaatcaccg ggacattaaa ctgcttcgcc
    15181 accgacgcaa cgcccagcgg cgctttaccg cctgccgttt gcgagtcaat gcgcccttcc
    15241 ccggtgatca ccagtgctgc gccctgcacc gcctgctcca ggttgaccgc ccgcagtaca
    15301 atctcaatac ccggtttaat atccgcattg aggaataccg ccgccgcaat acccataccg
    15361 cccgcagcgc cgccgcccgc catctggcag acattaattt cagtttgctg ttgtaaaaca
    15421 cgggcgtaat tttgcagccc ctgttcaagt tcttcgacca tctccggcgt tgcccctttt
    15481 tgcgggccaa acaccgccgc cgcgccgcgt gcccctacca gcgggttatc aacgtcacat
    15541 gctacttcaa tatggcaatt cgccagacgc ggatcgcatt catccatctc aatgcttgcc
    15601 acgcgcgcta aattaccgcc gttagccgcc agcacctggc cgtcggcatc aaggaaacgc
    15661 acaccaagcg cctgagccat gcccataccg ccgtcgaccg tcgcactgcc gccaatgccg
    15721 agaataatat ggcgaatgcc gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa
    15781 ctggaggcca gcaacggatt acgcttttca ggcgcgacca gcatcaggcc acttgctgcc
    15841 gccatctcaa tcaccgccgt tttcccgtcg ccggtaaggc cataaaaagc attcactgtt
    15901 tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc
    15961 gcatccaccg tcccttcgcc gccatccgct atcggcaaac agatatagtt cgcatcggga
    16021 aagagggtcg aaaacccggc tttaattgcc tgacaacatt tttctgcact taagctctct
    16081 ttaaaagagt ctggcgcaat gacaatcttc attatcaatc ctttttcaac aaattattcg
    16141 atgcgttccc acggaataag attatccata agaacagcca gtaaaattcc gaccagtaag
    16201 ccgttactga gcagaggacg cagcgttaat ggaaggtctt gcagatacac aggcggtaat
    16261 gccattaaaa atatgccgac aaataacggc aatgcgagtc gataaatatt gcgagcggta
    16321 aaggttattt gctggctaaa caccagcgcg gaaaagagta aaggcagata agaaaccagc
    16381 atgaccgcgc tactcacggg taaagggata ctgcaaaaca gtcgtgtgag tgccggaacc
    16441 agcgccacca gcaggcaaat aacgctgcca taaataaacg aacgccgcgt gtaatcgcct
    16501 gtctgggtta ataaaccaat ggatgaaaca aacggtgaaa atggaattac cgccagcggt
    16561 acggttatca gcgtcataaa tccggtcgcc acaaagctgc gacgataacg cgtattcccc
    16621 gcgccctgct gcggataaaa aacatccgtg ccccgaatcg caccgtaggt attgctgata
    16681 ttcaccagac ctgtaatcac cgccgtcaga ataattcccg gagacaaagc acctccactg
    16741 ccgagcggga accactgcca gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa
    16801 aagtaccaca acaaccagcc ggttatggtg ccgaccagca ggccataacg ggcaaaacgt
    16861 tgcggcagga agataatcat ccccagtacc aggcacatca ccgccaccga gagcgcgaac
    16921 ggcggtaact gaattttaaa atttgggtcg gctatgccaa acggcagccc gagcatacct
    16981 ttgaaaaaga tagtggtcag ctgcgcgccc agcatcaaca taaacaagac catcaccgac
    17041 ggcgtaaata accgtgccag gcgatggcct aatccgctaa aaccaatcaa catcgtcagc
    17101 acgccggaga gcgcaatccc caccgccagg ctggtagcga tatcgttgat cggtgtcccg
    17161 cgcgatgctt cgccaagggt gatagtaagg atggttcccc accacaggcc acccggccct
    17221 tccataatag cgcgacgatg accgcagaac gcctgagcga agcaggccag tgcggtagca
    17281 agaaaagcat attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt
    17341 agcgtaggag gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat
    17401 aacaggcttt cgcggctcac tgcaaaattg aacataaaca caccttaacc ggagaatgcc
    17461 cgccgcaagg acgggcattg caggggccag attactgctg atgtttaagg ataaattgac
    17521 ctttcggcgc aacagggaag ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg
    17581 ttttcgtgat acgcgcgcca atggtacggc caacatacgg gctgactttg tggcgatatt
    17641 ccagatcgtc attggtaaga acatagctgc tattcggctg aatgaagacg aagtcggcat
    17701 cttttcctgg ggcgatacgg cctttttgct gcagaccgaa aatatctgct gcgttagtcg
    17761 ccattaattt gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca
    17821 tcacgtccat acagttttgc agaccggcaa taccgcccca tgcttccatg atgttgccgg
    17881 ctttcatttc cggagggcat ggtgagtggt cggaaaccag gcagtcgatt tcaccgttaa
    17941 acagtttttc ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg
    18001 ccagagtgcc gatttcttcg aactgatcgg tatccagtac aaagtaatgc gggcaggatt
    18061 cacaagtaac gtcctgacct tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc
    18121 tgctgatatg gcaaacgtgc agacggcaac cggcaacttt cgccagatac agcacgcggc
    18181 gaatcgcttc cacttcggta aataccggac gcgaagccac atagtcatgg gcagttacgc
    18241 gaccttcacg tttcgcttct tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca
    18301 ccagcaccgg ctgccccagt tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt
    18361 ttacatcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga
    18421 agccgacaac gcccacttca tccagctcat gcagacggtc gatgttgtaa gacaccaggc
    18481 caccgagttg tgccgcatcg atagtcagct tgcctttagc ggcatcgaac ttcagttcaa
    18541 ttgaagcgcg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc atggtggtga
    18601 taccaccttt tgccgctgcg cgagtaccgg tttcataacc ttcccagtgg ctacgacccg
    18661 gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga ccagacgcat
    18721 ccataacttc ttttgcatcg cccagatcct gaccgatagc agcaattttc ccgcctttaa
    18781 cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta
    18841 aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact caatgttcaa
    18901 tatcgggatt aattaaccta tggttttttg ctctcctgtt ttttctgctg ttgtacgttt
    18961 ctttaataag gcgtaggccg caaaggcgac gatgacgccg acaaaccatg aaacacgcga
    19021 taacggttcc ataaagtgaa taaacttacc gccaagagat aaaataacgg ccaccagagt
    19081 tactgaaaac gcagtgaggt taaaaccgtt atcgtaatat ttataatcgc caggtgcggt
    19141 atacagttca tcaagattaa tttgtccgcg catcaccaca aaataatgcg ccatcatgac
    19201 accaattacc ggaccaagca ttccgccgat aatatcgagg aaaagataaa tgctgtcctg
    19261 attttccatt aatttccacg ggcagatcag caagctgata atactggcaa tcagtacgcc
    19321 gtttttatag gtcagttttg tcggtgcaat ggcggcaatc tgataaccgg ctggaataat
    19381 attaccggtc gcgttagtgg agatagttgt catcagaata accagtaccg caaagaacga
    19441 ggcgaacagg ctgtcccaac gctgaacaat atccagcacg ttccaggtat cagcgccgta
    19501 gtgaatactg gctccggcaa taatacatac cccggcgacc gcaaacagaa tataggccac
    19561 aactaaaccc agcgtttgcc ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa
    19621 gtcggatgcg ctcaccgccg gtgccgccca gaccgcaact accgcgttaa tcaccaccag
    19681 gaacaggaag ccaccgtttt ctgctttctg aataccgctc ggaatgtagt caaagattgg
    19741 accgatcccg accagtgaaa tcgcccaaat cgccataccg ccgaaaacga tatagatgca
    19801 cgggttaaga atggcagtga atttatttaa aactttgcca ccgccaaaac ctataccaac
    19861 gttgaccagc cagaagatta agaaagtaat taagcccggt agagaaaggc ctaacagagt
    19921 gaaatcacca ccgagagtta aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc
    19981 cagtgacccc gcgtaacatt gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa
    20041 taatccggga aacagtgcac cacgtacacc gtaagaagca cgcaggatca tggcaaaagg
    20101 cacaccgtat ttactgcccg cagcaccgtt taataccatt accgcggcaa tgaaaaaggc
    20161 gctgaggata attgccagca taatactaaa ggtagacaag ccgagaataa aaaagccgcc
    20221 gaccatcaca taattgggaa cgttatgaac cgaacccatc cataaggtaa aatagttaaa
    20281 tgttttccag gtccgctggc tttgcgtttt cggcaataga tcttcgctat agccgcgttg
    20341 ctggaatagt tttctctgat gttccataaa tccctcttaa tttcacgtat cgaataacgt
    20401 tgttttaatc tagctatgag gaacaaataa aataaaaata ttatttttaa tactgaagca
    20461 acatctttgg ctgctttctt ttttaatatt gctggccgat tcattttctg atatcggcca
    20521 tttttattgc gggtatcagg ccagtttatg gttagccatt aattccagcg cctgcactaa
    20581 cgcagagtga tccaactggc tgccaccgtt tgccgcgcag gtattaaata actcctggca
    20641 ggtcgcagtg tttggcaggt tcagcgctag cgctttcgca ctttgcagtg ccaggttgag
    20701 atctttctgg tgcagcgcaa ttttgaaacc tggattaaaa gtgcgtttaa tcatgcgctc
    20761 accgtgaact tccagaatac gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg
    20821 taccgggtcc gcaccggctt ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt
    20881 gagcgccaca ataatctgat ttgccacttt gcaggtttga ccatcgccgt taccgcccac
    20941 gagggtgata tttttaccga gcagttcaaa cagcggttta acacgttcaa ataccgcttc
    21001 atcaccgcca accataatcg acaacgtccc ttcacgcgca ccgatttcac cgccggagac
    21061 tggcgcatcg agataatcgc cgcccagttc attcacctga cgagcgaaac gcttagtttc
    21121 aatcggggaa atggagctca tatcaacaat ggttttgccc ttcagcgagg ctttggtaca
    21181 accattttca ccgaacagaa cttcttcaac ctgtggtgta tctggcacca taataaaaat
    21241 gatgtccgat gcttccgtta cctggcgcgc agtttcaaca ctgacggcac ccagtgacag
    21301 taattcatca gcaaccggac caatggttgt gacatgcagt tgatgaccgg cacgcgccag
    21361 attaatggcc atcggtgtac ccataatgcc taagccaata aatcccagtt tcataattaa
    21421 cctcttttaa atttcgcttt tcctgaattc agacaacacg atcccctgcc aacttagcgg
    21481 cctaaaaaag cattgcctga acagcgttac gttttaacgg tacggatcca tccagcgtaa
    21541 accggcttcc gtggtggttt ggggtgtata ttcacaccca acccaaccgt tgtagtcaga
    21601 attttcgatt accttaaaga gataatcata attaatttct ccggtccccg gttcgccgcg
    21661 atgcggatta tcagcaattt gcaggtgacc aattttatcg gcccactgag tcatggtgtt
    21721 ggttaattca ccttccatcc gctgcatgtg ataaatgtca tactgaattt tcaaattgca
    21781 gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg
    21841 aatatcaaaa tggttaatag gttcaatcag taataaaata tcttctttca tcagcatatt
    21901 cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc
    21961 cggcgtttta ccgaccagac agttaatttt tttattaccc agcgcacggg cataacgaat
    22021 cgctgctgct acgccatccc gaaactcttc ttcacggcca ggaatacagg caataccgcg
    22081 ctcccccgcc gcccagtcac ctgccggtaa attgtgcagc gtatgttcga gtttattact
    22141 cgccagcacc tgttttaatt cttcaatgtc gtagtcataa ggaaacataa attcaacgcc
    22201 gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt caccaaataa
    22261 catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa
    22321 gcaggtttca gtcggtgcgt ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt
    22381 atccagttcg ctgcccatcg aaatattggt cacacgctca agaataactt ccacgactac
    22441 cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg caatatcttc
    22501 cggtttgaag acccgaatag ctttacaacc taaaccttcc gctactttta cgtggtcgac
    22561 gccgtaaccg ttcacttcac tggagttaat attttcgaac gcgagctgta cgcagtaatc
    22621 catgtcaaag gcgcgttgtg actgacgaat cagccccaga taagcgttat tgaccagtac
    22681 atggatgtac ggaatgttga actgtgcgcc aacggctaat tcttcaatca ggaactggaa
    22741 gtcgaagtca ccggaaatcg ccaccacatt ccgttctgga tcagcagcac acacgcccag
    22801 cgccgccgga atggtccagc ccagcgggcc tgcctgacca cagttgatcc agtgacggtc
    22861 tttaaagaca tgcagcattt gtgccgcagc gatttgtgac aggccaatgg tggtgacata
    22921 acaaacatcg cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg
    22981 cacgttgtcg aagtgggttt tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac
    23041 ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca cttcaaccag
    23101 cagtgtcagc gccgctttag catcagagac aatgccgaga tccggacaca gcacgcgacc
    23161 aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt atttctctac
    23221 cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt cggacgccag
    23281 cagcgttgcg ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg ccatcagttc
    23341 atgatcgtcc gggatacagc cccagcccat cagcgttggg atcaccggaa cgctggtcag
    23401 ttcagcaaac tgttgtaaca gtgcagctgc gtcagcatta attaccccgc ccccggcaac
    23461 aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct gcatacggct
    23521 ggcagcaggt ttgtagaccg gcagcggttc gtacatatca ggatcaaact cgatttccgc
    23581 aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag aacgcatcag
    23641 gtgaaatgcc tgttgcaaca cacgaggcac cagcgccgct tcacgtactg taaccgccat
    23701 tttacttacc ggcttggcga tagcttcgat atcaacagcc tggaaatctt ctttatgcag
    23761 acgggcgcgc ggagcctggc cagtaataca cagaatagga atggaatcag cagaagcaga
    23821 atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac atacgccgat
    23881 attccctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt ccacatgacg
    23941 cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg gattgattgc
    24001 agctcccgga acgccgaagg cggtggtgat accttctttc tccagcacat acattgccgc
    24061 gtcaacggct ctcatttttg ccattttatt cctacctcta ttaattggaa aaattttcca
    24121 actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag acactatgga
    24181 tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc
    24241 tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga gatagcggca
    24301 acaacgctac caacatcatc gtaaattgct gaagctatgc aattcagacc tacaacatgc
    24361 tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg
    24421 ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc
    24481 atcaactcct cttccgccag cggataaagc aacgccttgc ccgcaccgga agcatgcagc
    24541 ggcagacgac tgcccagtgg cgcacacatc ctgaccatcg atttacactc taactgacca
    24601 attaataccg cttcattgcc gttacggatc gcgacattga ccgtttcgcc ggaaagtaac
    24661 atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg gatgtacgcc
    24721 gcaccgacgt taaagacacc taatcctata tgccaccagc ctaactgact gtcctgatag
    24781 acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg tcgtagagag cggcaaatcc
    24841 agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccag atattgcaga
    24901 atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct
    24961 ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta caagtgagca
    25021 taaacaatga acaattagtt tatcttatgc aagtcggttg caggcgtcac gcaaaacaga
    25081 gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga tcgatggtca
    25141 gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta cggtggtaat
    25201 tcactccctg ttcgccgttg gtaataaacg cccgcagggt tgacaggtct ggtttgtcgt
    25261 cacctaacgc cacgaccacc acaaacacct cacctttcat cgggataaag gcctgagtac
    25321 ccagcggatg acgttcgagt tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt
    25381 taatgctgat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac
    25441 gctccaccag gccattgtta atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc
    25501 cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc tttttgtcct
    25561 tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tggtttcaat aacaggcaat
    25621 ctaaaacctt aagagaacaa cattaataca aaagttggaa tatttttcca aaactagatt
    25681 tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga ggataagaaa
    25741 atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca gaaaccttgc
    25801 ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa cgattatgta
    25861 aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc ggagtagcgc
    25921 tgtttttccg cacgactcgc agcgtgacgt tgacagcggc tggcgagcat ctactttccc
    25981 aggccagaga ctggctgagc tggctggaaa gtatgccaag cgagctgcaa caggtgaatg
    26041 atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctacaac ccccaggccg
    26101 tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt tacccagttt cacatctccc
    26161 gacaaatcta tatgggcgtc tgggattcgc tattatacga aggtttttca ctggctatcg
    26221 gcgtcacggg aaccgaggcg ctggcaaata cctttagtct tgatccctta ggatcggtgc
    26281 aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag ccgctaacag
    26341 aagcgcagtt gcggcgcttt ccggcggtca atattgaaga cagcgcccgc accttaacca
    26401 aacgcgtcgc ctggcgattg cctgggcaaa aagagattat tgttcctgat atggaaacga
    26461 aaatcgccgc ccatcttgcg ggcgttggca ttggtttttt gccaaaatcg ctttgccagt
    26521 caatgatcga taatcaacaa ctagttagcc gggtaatccc aacgatgcgc cctccttcgc
    26581 cattgagtct ggcatggcgc aaatttggca gcggcaaagc ggtagaagat attgtgacct
    26641 tgtttaccca gcgcaggccg gaaatcagcg gatttttaga aattttcggc aacccacgca
    26701 gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg
    26761 ttcactttta actatgcaag agagacacac ggaacaggac tatcgtgccc tgctgattgc
    26821 tgatacgccc attattgatg ttcgcgcccc tatcgagttt gagcaaggcg caatgcccgc
    26881 cgctatcaat ctgccgttaa tgaataacga tgaacgcgcc gccgttggca cctgctataa
    26941 acagcaaggc tcagacgcag cgctggcgct ggggcataaa ctggtggcgg gtgaaattcg
    27001 tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg
    27061 ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc tggttgtatg cagcggggat
    27121 tgattatccg ctggtggaag gcggttataa ggcactgcgc cagaccgcga ttcaggcgac
    27181 tattgaactg gcacaaaaac cgatagtgct gattggcggt tgtactggca gcggtaaaac
    27241 gctgttagtg cagcaacagc cgaacggtgt tgatctggaa gggctggcgc gccatcgcgg
    27301 ttcggcgttt ggtcgcacgt tacaaccaca acttagccag gcgagttttg aaaacctgct
    27361 ggctgccgaa atgctgaaaa ccgacgcccg tcaggatttg cgcctgtggg tgctggaaga
    27421 cgaaagccgg atgatcggtt cgaatcacct gccggaatgc ctgcgcgagc gaatgactca
    27481 ggcggcgatt gcggtggtag aagatccgtt tgagatccgt cttgagcgcc tgaacgaaga
    27541 gtatttcttg cgtatgcatc atgattttac ccacgcttat ggcgacgaac agggctggca
    27601 ggaatattgc gaatacctgc atcacggact ttcggcgatt aagcgtcggc tggggttaca
    27661 gcgctataac gaactcgctg cccagctgga tacggcgctg acaacgcaac tcaccaccgg
    27721 cagcaccgac ggtcatctgg cctggctggt gccgttactt aaagaatatt acgacccaat
    27781 gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga
    27841 agtggcggaa tgggttaagg cgcggtaatg agggacgtat ttgtattagt cgcgacttga
    27901 tctgacacat ggccttgaaa ggttgagagt taccggtttt gatatgagtg tcgaatcctt
    27961 atccaaaaca agaggtaact ctcatgcttc atactgccaa tcccgtcatc aaacacaaag
    28021 ccggtttgct caatctggct gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg
    28081 gcgtttcgcg tgatacgttt tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg
    28141 cgcagattaa tcgtagtcgc cgcgctccta accttaagaa ccgtaccgat gaggcaactg
    28201 aacaggctgt tgttgattac gccgtcgctt tcccgacaca tggtcagcac cgggccagca
    28261 acgagctgcg taaacagggg gtttttatct ccgacagtgg tgtccgttct gtctggttac
    28321 tccataacct tgagaatctc aaaagacgat actgaacaaa ttttatcagg tgacgttccg
    28381 taaaaagttg tatggagttc tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc
    28441 ctggactatc gggccgccgt actggggcat gtcaccctgg cgtgccgctg gttttccgct
    28501 aatggtttat ttcctctttc tttcccggca agaatttatt ttaatccgga aggattattt
    28561 attttcagat agggactcac attaaacacg taacatttta attaatgcat ggctgaatat
    28621 gtattacaca atgtcagata tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact
    28681 gttcatttta tttgttgttt cagggtcgga actcaccctc aataaacaac tgaatacgag
    28741 tagatattca tcgtctgagc tatatggctt tacacaatag ccgacatgtc ttttctcagt
    28801 caggaatttt acttttcctc actacgaagt tgacgacttc gatatgggat agactcttaa
    28861 ttcaagcaat tactctgcac aagcatcagg caatccagct ccaatatttg ttataaaata
    28921 atgcgtaaca ttaatttcac caacaaaaaa aaggtttaca ccatcccctt gccctggtat
    28981 cttaccatat ttacaccaca ctttttttgt atctggatca ttctttgata cgattttcgc
    29041 cacttgttcc ctggccctca ctgtcaatgt tttcttaaac ctacccccat catcatttgt
    29101 tagggaaaaa taagaaaaaa ataatcatct tctacatttg tgtatattga tatatccacc
    29161 gctacgtgcg gaaaaaaatc tgggagccat gtaaaaactc catcattttc tggtattgcc
    29221 atatagttta tgtaatg
//
LOCUS       DAZUFB010000055        28961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZUFB010000055 DAZUFB010000000
VERSION     DAZUFB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.055"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..552)
                     /locus_tag="ACHY3M_002723"
     CDS             complement(<1..552)
                     /locus_tag="ACHY3M_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HFS4317614.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVT"
     gene            complement(579..1784)
                     /gene="paaJ"
                     /locus_tag="ACHY3M_002724"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(579..1784)
                     /gene="paaJ"
                     /locus_tag="ACHY3M_002724"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFS4317615.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(1784..2206)
                     /gene="paaI"
                     /locus_tag="ACHY3M_002725"
     CDS             complement(1784..2206)
                     /gene="paaI"
                     /locus_tag="ACHY3M_002725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFS4317616.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(2196..3623)
                     /gene="paaH"
                     /locus_tag="ACHY3M_002726"
     CDS             complement(2196..3623)
                     /gene="paaH"
                     /locus_tag="ACHY3M_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFS4317617.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(3625..4413)
                     /gene="paaG"
                     /locus_tag="ACHY3M_002727"
     CDS             complement(3625..4413)
                     /gene="paaG"
                     /locus_tag="ACHY3M_002727"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFS4317618.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(4413..5180)
                     /gene="paaF"
                     /locus_tag="ACHY3M_002728"
     CDS             complement(4413..5180)
                     /gene="paaF"
                     /locus_tag="ACHY3M_002728"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFS4317619.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(5177..6247)
                     /gene="paaE"
                     /locus_tag="ACHY3M_002729"
     CDS             complement(5177..6247)
                     /gene="paaE"
                     /locus_tag="ACHY3M_002729"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFS4317620.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(6255..6752)
                     /gene="paaD"
                     /locus_tag="ACHY3M_002730"
     CDS             complement(6255..6752)
                     /gene="paaD"
                     /locus_tag="ACHY3M_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFS4317621.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(6767..7513)
                     /gene="paaC"
                     /locus_tag="ACHY3M_002731"
     CDS             complement(6767..7513)
                     /gene="paaC"
                     /locus_tag="ACHY3M_002731"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFS4317622.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(7522..7809)
                     /gene="paaB"
                     /locus_tag="ACHY3M_002732"
     CDS             complement(7522..7809)
                     /gene="paaB"
                     /locus_tag="ACHY3M_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFS4317623.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(7821..8750)
                     /gene="paaA"
                     /locus_tag="ACHY3M_002733"
     CDS             complement(7821..8750)
                     /gene="paaA"
                     /locus_tag="ACHY3M_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFS4317624.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            9035..11080
                     /gene="paaZ"
                     /locus_tag="ACHY3M_002734"
     CDS             9035..11080
                     /gene="paaZ"
                     /locus_tag="ACHY3M_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFS4317625.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            11328..13601
                     /gene="tynA"
                     /locus_tag="ACHY3M_002735"
     CDS             11328..13601
                     /gene="tynA"
                     /locus_tag="ACHY3M_002735"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFS4317626.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(13659..15145)
                     /gene="feaB"
                     /locus_tag="ACHY3M_002736"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /pseudo
     CDS             complement(13659..15145)
                     /gene="feaB"
                     /locus_tag="ACHY3M_002736"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
     gene            15381..16286
                     /gene="feaR"
                     /locus_tag="ACHY3M_002737"
     CDS             15381..16286
                     /gene="feaR"
                     /locus_tag="ACHY3M_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFS4317627.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADNEKLAGICFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(16458..16787)
                     /gene="ydbL"
                     /locus_tag="ACHY3M_002738"
     CDS             complement(16458..16787)
                     /gene="ydbL"
                     /locus_tag="ACHY3M_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFS4317628.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(16792..16977)
                     /gene="ynbE"
                     /locus_tag="ACHY3M_002739"
     CDS             complement(16792..16977)
                     /gene="ynbE"
                     /locus_tag="ACHY3M_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFS4317629.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(16974..19613)
                     /gene="ydbH"
                     /locus_tag="ACHY3M_002740"
     CDS             complement(16974..19613)
                     /gene="ydbH"
                     /locus_tag="ACHY3M_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFS4317630.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQMEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            19821..20810
                     /gene="ldhA"
                     /locus_tag="ACHY3M_002741"
     CDS             19821..20810
                     /gene="ldhA"
                     /locus_tag="ACHY3M_002741"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS4317631.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            20921..21343
                     /gene="hslJ"
                     /locus_tag="ACHY3M_002742"
     CDS             20921..21343
                     /gene="hslJ"
                     /locus_tag="ACHY3M_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFS4317632.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(21340..21606)
                     /gene="ydbJ"
                     /locus_tag="ACHY3M_002743"
     CDS             complement(21340..21606)
                     /gene="ydbJ"
                     /locus_tag="ACHY3M_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFS4317633.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            21880..25404
                     /gene="nifJ"
                     /locus_tag="ACHY3M_002744"
     CDS             21880..25404
                     /gene="nifJ"
                     /locus_tag="ACHY3M_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4317634.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     GRIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            25771..26904
                     /gene="ompN"
                     /locus_tag="ACHY3M_002745"
     CDS             25771..26904
                     /gene="ompN"
                     /locus_tag="ACHY3M_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFS4317635.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGVSNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVDLGLVYQF"
     gene            27045..27479
                     /gene="uspF"
                     /locus_tag="ACHY3M_002746"
     CDS             27045..27479
                     /gene="uspF"
                     /locus_tag="ACHY3M_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFS4317636.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(28060..28701)
                     /locus_tag="ACHY3M_002747"
     CDS             complement(28060..28701)
                     /locus_tag="ACHY3M_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFS4317637.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            complement(28783..>28961)
                     /locus_tag="ACHY3M_002748"
     CDS             complement(28783..>28961)
                     /locus_tag="ACHY3M_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFS4317638.1"
                     /translation="MRNSRGAEICSLYDKDALVQLVETGGAHPLSREPITESMIMRKD
                     ECHFDTKREAFCCK"
BASE COUNT         7164 a         7604 c         7013 g         7180 t
ORIGIN      
        1 cgtaaccatg atcatatctg gttgaaaatc gcggatcagt tgcgcttgtt tttccgtctg
       61 cccaccagac atcgggatca ccgtagcgcc cagacgttcg gcaccatagt gcgcgcccag
      121 cccaccagta aacaggccgt aaccataggc aacatgaatt ttgtctttgg gcgtgccacc
      181 tgccgcacgc aaggaacgcg ctacaatatt ggcccagtta tcaatatcgt tttgcgtata
      241 gccgacaact gtcggtttac ctgtggttcc cgaagaagca tgaatgcgca ccacttgttc
      301 catcggcacg gcaaaggtgt cgaagggata gttgtcgcgc agatcctgtt tggtggtaca
      361 gggaaattta cgcagatctg aaagttccct gaaatcatca ggatgtacgc ctgctgcgtc
      421 aaatttgcgc cgatacatcg ggacattttc atacgcgtgt ttgagcgtcc atttcagacg
      481 ttgtgtttgc aacgcctgta actcatcaac agacgcggtt tcaatcgggt caagctttgt
      541 attggttatc attgtagggt actcgcaggt tgatatgctc aaacacgctc cagaatcatg
      601 gcgatgccct gaccgacacc gatgcacatg gtgcacaatg cgtaacgacc gttacgccga
      661 tgcagctcat ggctggcagc cagtgccagg cgggcaccac tcattcccaa cggatggcct
      721 aaggcgatag cgcctccgtt ggggttaaca tgtggggcat catcaggcag ccccaattcg
      781 cgtagtacac ccaacgcctg ggccgcgaac gcttcgttca gttcaatcac gtccatatcg
      841 tgaatactca gccccgcgcg ttccagcacc cggcgagttg caggcaccgg accaagcccc
      901 atcaggcgcg gttccacccc ggcggttgcc atggctacga ttcgcgcccg cggtgtcagt
      961 ccttgcgctg ctgccatctg ttcactggca ataatcaacg cagcggctcc gtcattcacc
     1021 ccggaggcat tgcctgcggt aatcacccca ttggcacgaa atggtgcttt taacccacgt
     1081 aactgttcca gcgtcgtttc cgggcgcaga tgctcatcat gttgtatttc tgttacaacg
     1141 cctttcttgt ttttcaacac aaccggaaca atctcctcag ccagaatgcc tgaggattgc
     1201 gcttttgccg tacgttgctg actgcgtagc gcaaaactat cttgatcttc tcgcgatatt
     1261 tttaacagtt cagctacatt ctctgccgtt tccggcatgc tgtcagttcc aaattgttga
     1321 gccatgagcg ggttcacaaa tcgccagcca atagtggtat caaacatctc agcctgacga
     1381 gaaaatgcac tggttgcctt gcccataaca aacggtgccc gagacattga ctccacgcca
     1441 ccagcgatca gcaaatcgcc atcgcccgct ttaatcgccc gtgcggcgaa ccccagtgcg
     1501 tccagcccgg aaccacacaa gcggttaatg gttgtgccgg aaacactctg cggcagcccc
     1561 gccagtaacg tcgccatccg ggctacgtta cggttgtctt ctcccgcctg attagcacag
     1621 ccgaggatca catcatcgat acactccgca tcgagacgcg ggtttcgcac cagcagctcc
     1681 cgcaaaggga tagcagccag atcgtcagcc cgaacacctg ataatgcccc accatagcgg
     1741 ccaattggcg tacgaattcc gtcacagata aaggcttcac gcatcaggct tctcctgtaa
     1801 tggtgccgcc gatgcggtga gatttaccgc gaaacagcgc aaccgttttt tgttgttggt
     1861 taacaatttc gatgtcgtaa acaccggttt gcttgccctg atgacgtacc tgcgcagtag
     1921 cagttaaggt gtctccggca aagcctggac gcaaaaaatc aatcgtgcag gcagaagcga
     1981 cggctgccag cccctggcta ttgcaggcgt aggcaaaggc agtatcagcc agtgaaaata
     2041 gctgcccgcc gtggcaactt tgatgaccgt taagcatttg tgcagtgacg gtcatggtca
     2101 ctacagcaaa gccttcatcc attgagataa tgtcgatgcc aagcgctttg gcgcaggcat
     2161 cgttctcata cattgcatgg gcattttgcc aggccttatg actcataacc gctctccaga
     2221 agcgctcgtt ggcgcagcaa tgaacatggg cgatagcgtt cttcgccata gtgatgttgt
     2281 agattttcca ggagccttaa tattcgctgc cagccaagtt gcgctcccca ggcaagcggg
     2341 ccatatggat aattcacccc aagacgcatg gcggtatcga tatcctgttc agaggccacg
     2401 cctttttgca gcgcatcaag ggcttcattg atgatcattg ctaccgttcg ccaaatcagc
     2461 attcctgggt aatctgcaat ttgcagcact gttttgccct gctgttgcag gtaataaatg
     2521 gccttgcggg tcgctgagtc cgggttcacc gctgccgcag caatggtcac caccttgccc
     2581 gccattttgt cgaccactac caccgggcgt gccagtcgta ttgccagcgc ctgtgccgtc
     2641 tcgccttgtg tctcaatcaa taaaacatcg tcaatttccg tgacgccgtc acttttcttt
     2701 tctactttca ttgggctaaa actgtcgctt accgcttcca ggccaacaac tgcctcacgt
     2761 tccgcgcgcc agtcgtacac gcccagccca cttttcttgc ccaaccgtcc accaatcacc
     2821 agttcctgtt gcaccagcga aggtaaaaaa cgacgctcct gccagaaagc gttaaacacc
     2881 gaacaggtga cagcaaaatt gacgtcctga ccaatcagat cggttaattc cagcggcccc
     2941 atcgggaaac cagcgccatc gcgaagtgca gcgtcaatca cttctggtgc agcaacctgc
     3001 tcttccagtg cccgccaggc ctcggaataa taaggacgcg ccacacggtt aacgataaat
     3061 ccaggggtcg aatgacagcg cacaggctgc ttaccccaac tcaacgttag ttcacacaac
     3121 tgctcaacaa cttccgctgc cgttgccagc ccactgacca cctccaccaa cttcatcacc
     3181 ggtgccgggt taaaaaaatg cagccccgca acacgttcag gattttttac ctccgcagca
     3241 atcgcggtta tagagattga cgaagtgtta gtggtcaata gcgtttgtgg cgggcaaact
     3301 tccgccagct gtgcaaagag cgcttttttg acttccagac gttcagacgc cgcttcaatg
     3361 accaggtccg cagctgccag cgcgtgaata tcggtcaccg ggatcaggcg tttcaatgtg
     3421 cgttcacagg tttcagcagt cagttttccc cgcgtcacgc gtgaatttag ccgcgcgtgt
     3481 atcccgtcga ttgcgcgggt cagcgcttca gcagaaatgt catacagtaa aacctgatgt
     3541 ccatgactgg cggcaacttc agcaatgcca gcccccatgg tgccgctccc aatcactgcc
     3601 acagtttgca catttatcat catgctattt ccccgtgaac tgcggtgagc gtttagccag
     3661 gaacgcactg acaccttcac gataatctgc gctccgtccg gcaagtcgct gatagtcgcg
     3721 ttccagatcc agttgcgtat cgagcgtatt ggtttcagcg ctatttatcg cttgcttgat
     3781 aagtccaaga ccaaatgtcg gttgtgtcgc cagatgccgt gccagctgtt gcgcggtatc
     3841 tgccagcgtt tcatcatcaa caacctgcca gatcatcccc cattcgtgcg cctgttcagc
     3901 actcagttga ttccccagca gtgccagccc catggcgcgc gctcgcccag caacgcgtgg
     3961 cagtaaccag gttccaccgc aatcgggtat taagcctaac ttactaaacg ccatgacgaa
     4021 ttttgctgaa cgggcagcaa taacgatgtc gcaccccagt gccagtgttg cgcctgcccc
     4081 tgccgccacg ccattgactg cacagatcac cggttttggc agttttgcca ggcgacgtac
     4141 cagtgggtta tagaaacgtt caactgacat ccctaaatcc ggtgcggggc ctgtgggatc
     4201 aacgttacga tcgttaagat cctgaccagc acaaaatccg cgcccggcac ctgtaagtaa
     4261 caggcaacgg atagtgtcgt cgcgctcgac ctgtttcagg cactctgcca gttgtgcgtg
     4321 catctcatca ttaaaactgt tcaggcgttc cgggcggttg agcgttagtg tcatcacgcc
     4381 cttttctaca tgactgagga tgaattccat cattagcgtc ctttaaagtc gggcgtgcgt
     4441 ttttgtaaga aagcggagat gccttcatga cgatcttctg ttgccgccag caaggtgaat
     4501 aactgtcgct cctgggcaag tccggcttgc aaagccactt cctgcgactg gcgcagcgct
     4561 tgctttgccg cttgtaaggc caacggcgag tgacgtgcca ttttcgatgc cagctgtaag
     4621 gcgtattcga gggttaaatc gctggggaag acgtcgctaa ccagcccggc ctgctgtgct
     4681 tgctgggcgg tgatactttc tccgctcagc accattttgc tggctaacga tttaccgaca
     4741 ctacggatta aacgttgcgt tccgcctgcg ccaggcatga tgccgagagt gatttccggc
     4801 aacccaaaac gcgcgttctc tccggcaacc accacatcgc acaacaatgc cagttcgcaa
     4861 cccgccccaa gcgcgtaacc attgacggct gcgataagtg gtttattaaa ggcctgcaat
     4921 cgcgcccata gctgcggacg tgtatcgttt aaggtggccg cgagatcttt ttctgccatt
     4981 tcgttgagat cggccccagc ggcaaaaaag cgtgcattac cggtaatcac acagaccgaa
     5041 atgctggtat cggtagccgc agcttccagt tcatttacca gttgcatcag cagggcatta
     5101 tttagcgcat tacgtgcggc gggacggtta agggtcagca acaatactcg ttgctgacgg
     5161 ctgacgatca gttcgctcat gccatcccct tcgcgtcaaa gtcaaccacc acatcgctgg
     5221 tcagcggcag tgcctggcaa ctcaacacat aacctgcggc cagttcatcc ggttccagac
     5281 tgtaattggt ttccatcgcc actttgccac gcagcacttt gcatttgcag gtcgcacaga
     5341 cgccgccttt gcaggcatag ggcagatcag ccccctggcg caatgccgca tcgagaatgc
     5401 tttcatcgtc ggcattaagc acgatttccc gatcccgccc atcctgacgt acagtcactt
     5461 tttgtccgtc actttgcacg ttaacgctac gtttgacgcg cgtgccaggc gtattaaacc
     5521 gctccagatg aatggtttta tctggcattc ccagtgcttt taaggcggct tccgtctcat
     5581 ccatcatcgc cgccggacca caaataaatg cctcatcata aagacgaaaa ttaatgagcg
     5641 aggccccaag tgactgtaat ctttcaccgt caatacgccc gtgaagcaga tcgctgtcga
     5701 gggtttcctg actgaaaatg cacaacaact gtaaacgctg aggatatttg tctttcaggt
     5761 ctgccagtgc ctggcgaaac atcatgctct ggctggtacg gttaccgtag atcagggtga
     5821 actgactttc aggctcggtt tgtaaagtgg tggcgataat cgccagcatt ggcgtaatac
     5881 ctgatcctgc tgcaatagcc agatagcggc cctggcgttc ggcctgtggc tgatagccga
     5941 aatgcccctg cggcaccatg acctccagcg tcataccctg gcggatgtgt tcgcgggcat
     6001 agcgggagaa acgtccgcct tcaatggctt tcaccgccac actaatttcg ccaggcaggt
     6061 agctgcggca aatggagtaa caacggcgta attcttcacc atcaagactg gcttttaagg
     6121 tcaaatgttg accggggcga aagcgatacg cctcctgcaa aggctggggc accgcaaagg
     6181 taatggtcac cgcatcacgg gtttccggct cgacttttgc cactgttaag gaatgaaacg
     6241 ttgtcatggc atcctcaaat acatttgaaa taatcgaaag gttcgcggca actatcgcag
     6301 cggtacaatg ctttgcaggc cgtggaacca aattcactga taagtgtggt atggacgctg
     6361 gcgcagcgcg ggcaacgtac ttctggcggc aaatgggcat ggcaactgtg tccggcgggc
     6421 gggctaatgc catactgtcg cagacgctca cgggcatcgg gggtcatcca gtcggtggtc
     6481 catgccgggt cgagttgcag cacaacctga acgggggtaa acccgtgggt tgtcattgcc
     6541 tcacgtatcg cgccaatcaa atgttccgtt gccggacaac cggaataagt cggcgtaaag
     6601 ccgatcaccc atccttctcc catctgtgtc acattacgca ccatgcctaa atcggtaatg
     6661 gtcagcaccg ggatctccgg gtccgggatc tggctgagta gcgcccatat ctcatgaacc
     6721 tgtggcggtg caatggtggc cagacgttgc atacccatct cctctgttac cattgctgac
     6781 cgggcaagac acgctggaga tactgcattt ctgccagcat gggtccaagg tgttcggtat
     6841 gcagtccttt tttaccgcca gtgcgatacg cctgctcttg cggtacgttc aatgtggctt
     6901 cgttgattcc ggcaaaaact tcggcttccc acgctgcgcg taaagtgcgt ggatcaaccg
     6961 caataccctc ttcactcagt gcaatatcaa tctcgtcggc atcgaacagt tcggcagtaa
     7021 aacgccacaa cttgtcgatg gcctgctgca tcttttgccc tgatacgtca gtaccattgc
     7081 ccagtcgctc cagccagcca cgactaaaac gcaggtgata acgcgcttct ttaattgctt
     7141 tggcagaaat cgccgccagt tgcggatcac ggctttccat cagacgggta aagagcgcca
     7201 catgccatgc atcgatgaaa tactggcgtg caatggtgtc ggcaaaattg ccgtttggtt
     7261 gttcaaccaa caataagttg ctgaactggc gctcgtctcg ggtgaaggcc agggtatctt
     7321 catcaccttc tcccgctaat tcagcggcat acgataagaa gttacgtgcc tgacctaata
     7381 aatcgaggcc aatgtttgcc agtgcgagat cgatttccag ttccggtgcg tgaccgcacc
     7441 attcccccaa ccgctgggag agcaccagac agttatcgcc caggcgcaag gtgtaagccg
     7501 ttaactgatt catttccgac ctcacatgtg ctcaatgcca tcagggatgg tgtaaaacgt
     7561 tggatggcga tagaccttgc tttcagccgg atcaaaaaat tcaccgcgtt cttccggttg
     7621 cgaggcaaca atttcactcg ccttcaccac ccaaattgaa catccttcgc tacgacgggt
     7681 gtaagcatca cgggcatttt ccagtgccat ccgctcatcg gcagcatgta aactgccaac
     7741 atggcggtgt gataagccct gtttgccacg cacgaaaact tcgtataacg gccagtaaac
     7801 attactcatt ttgaatctcc ttatgcgacc ttacgggcaa gttgtttttg tgcatgggcc
     7861 agcgctgctt cccgtaccca ggtaccttct tcccaggctt tacgtttggc gtcgagcctc
     7921 tcctgattac aaattccgcg accgttaatt acttcgttaa actcctgcca gtcgatctca
     7981 ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat ccgggtcagg aacggtcata
     8041 ccgagcattt caacctgtgg aacggtgttg tccacgaaac gctggcggag ttcgtcgttg
     8101 gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt ttggcgagtt atcatcgttt
     8161 ggcccgaaca tcattaaggc tggccaccag aaacggttaa tggcgtcttg caacatctgc
     8221 ttttgagctt cgctgccctg cgccagtgcc atgcaggctt caaacccctg acgctggtga
     8281 aaactctctt ctttgcagat tttcaccatt gctctggcat acgggccata agaagttcgg
     8341 cataacgcca cctgattgac gatcgctgcg ccatccacca gccagccgat aacgccgata
     8401 tcagcccaac tcaatgtcgg ataattaaag atggaggaat atttcatccg tccgtcgagc
     8461 attttttggt agatgtcttc tctggcacag cccagtgttt cggcggcgct atagagatac
     8521 agaccatgac cggcttcatc ctgcactttg gctaacagaa tggctttacg ccgcaaggtt
     8581 ggtgcgcgag tgatccagtt accttcaggc aacataccaa caatctcgga gtgcgcatgc
     8641 tgcccaatct ggcggatcaa tgtctttcgg taagcatcgg gcatccagtc ctgcggctcg
     8701 atagccgttt cctgggctat ccgttgctca aagcgttctt cttgggtcac gtcatcacct
     8761 ttacgattcc agttttcgtg aattaattaa aaacacgaat cacgttgttt tatgaaagtt
     8821 acacaatggt taactaaagt cacaaatttt gtatacttga actgtgacag atttcgcact
     8881 ctattttgat aactcattat ttatcagtgc attaatgttt ttagtagttg atatacagat
     8941 cacgcagtta acatttcgtt aaaagatccc ttgcttttta tgattcgcga tttaactatt
     9001 agcaacagaa atgtgaaaca tctggagagt agcgatgcag cagttagcca gtttcttatc
     9061 cggtacctgg cagtctggcc ggggccgtag ccgtttgatt caccacgcta ttagcggcga
     9121 ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg gctgcccgcc agtttgccat
     9181 tgaaaaaggt gcccccgccc tccgcgcgat gacctttatc gaacgtgcgg cgatgcttaa
     9241 agcggtcgct aaacatctgc tgagtgaaaa agagcgtttc tatgctcttt ctgcgcaaac
     9301 aggcgcaacg cgggcagaca gttgggttga tattgaaggt ggcattggga cgttatttac
     9361 ttacgccagc ctcggtagcc gggagctgcc tgacgatacg ctgtggccgg aagatgaatt
     9421 gatcccctta tcgaaagaag gtggatttgc cgcgcgccat gtactgacct cgaagtcagg
     9481 cgtggcagtg catattaacg cctttaactt cccctgctgg ggaatgctgg aaaagctggc
     9541 accaacgtgg ctgggcggaa tgccagccat catcaaacca gctaccgcga cggcccaact
     9601 gactcaggcg atggtgaaat caattgtcga tagtggtctt gttcccgaag gcgcaattag
     9661 tctgatctgc ggtagtgctg gcgacttgtt ggatcatctg gacagccagg atgtggtgac
     9721 tttcacgggg tcagcggcga ccggacagat gctgcgagtt cagccaaata tcgtcgccaa
     9781 atctatcccc ttcactatgg aagctgattc cctgaactgc tgcgtactgg gcgaagatgt
     9841 caccccggat caaccggagt ttgcgctgtt tattcgtgaa gttgtgcgtg agatgaccac
     9901 aaaagccggg caaaaatgta cggcaatccg gcggattatt gtgccgcagg cattggttaa
     9961 tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc gtggtcggtg atcctgctca
    10021 ggaaggcgtg aaaatgggcg cactggtaaa tgctgagcag cgtgccgatg tgcaggaaaa
    10081 agtgaacata ttgctggctg caggatgcga gattcgcctc ggtggtcagg cggatttatc
    10141 tgctgcgggt gccttcttcc cgccaacctt attgtactgt ccgcagccgg atgaaacacc
    10201 ggcggtacat gcaacagaag cctttggccc tgtcgcaacg ctaatgccag cacaaaacca
    10261 gcaacatgct ctgcaactgg cttgtgcagg cggcggtagc cttgcgggaa cgctggtgac
    10321 ggctgatccg caaattgcgc gtcagtttat tgccgacgcg gcacgtacgc atgggcgaat
    10381 tcagatcctc aatgaagagt cggcaaaaga atccaccggg catggctccc cactgccaca
    10441 actggtacat ggtgggcctg gtcgcgcagg aggcggtgaa gaattaggtg gtttacgagc
    10501 ggtgaaacat tacatgcagc gaaccgctgt tcagggtagt ccgacgatgc ttgcagctat
    10561 cagtaaacag tgggtgcgcg gtgcgaaagt cgaagaagat cgtattcatc cgttccgcaa
    10621 atattttgag gagctacaac caggcgacag cctgttgact ccccgccgca caatgacaga
    10681 ggccgatatt gttaactttg cttgcctcag cggcgatcat ttctatgcac atatggataa
    10741 gattgctgct gccgaatcta ttttcggtga gcgggtggtg catgggtatt ttgtgctttc
    10801 tgcggctgcg ggtctgtttg tcgatgccgg tgttggtccg gtcattgcta actacgggct
    10861 ggaaagcttg cgttttatcg aacccgtaaa gccaggcgat accatccagg tgcgtctcac
    10921 ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa gaaaaaccaa caggtgtggt
    10981 ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg gtggcgctgt attcaattct
    11041 gacgctggtg gccaggcagc acggtgattt tgtcgattaa tcggtgaatg aagggcaacg
    11101 gccaatagtt gcccttttat ttcactaagt tttgtgacgt tgtcacatta tgcatgatgt
    11161 gtacatctat tttcagggca cccactgtat gaaaagctgg cacacctgcc aaacaacctg
    11221 gcaggtgcag gcaatcccct ttgcatcagt actgataatg tgaacctgac taaaccgccc
    11281 acagagcgcg gttgctaaca agaacacaac atctgacgag gttaataatg ggaagcccct
    11341 ctctgtattc tgcccgtaaa acaaccctgg cgttggcagt cgccttaagt ttcgcctggc
    11401 aagcgccggt atttgcccac ggtggtgaag cgcatatggt gccaatggat gaaacgctta
    11461 aagaatttgg tgccgatgtg cagtgggacg actacgccca gatctttacc ctgattaaag
    11521 atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc aattgttaat ggtcaacctc
    11581 tggcactgca agtgccggta gtgatgaaag acaataaggc ctgggtttct gacaccttta
    11641 ttaacgatgt tttccagtcc ggtctggatc aaacctttca ggtagaaaag cgccctcacc
    11701 cacttaatgc gctaactgcg gacgaaatta aacaggccgt tgaaattgtt aaagcttccg
    11761 cggacttcaa acccaatacc cgttttactg agatctccct gctaccgcca gataaagaag
    11821 ctgtctgggc gtttgcgctg gaaaacaaac cggttgacca gccacgcaaa gccgacgtca
    11881 ttatgctcga cggcaaacat atcatcgaag cggtagtgga tctgcaaaac aacaaactgc
    11941 tctcctggca acccattaaa gacgcccacg gtatggtgtt gctggatgat ttcgccagtg
    12001 tgcagaacat tattaacaac agtgaagaat ttgccgctgc cgtgaagaaa cgcggtatta
    12061 ctgatgcgaa aaaagtgatt accacgccgc tgaccgtagg ttatttcgat ggtaaagatg
    12121 gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta tcttgatgtc ggtgatggca
    12181 actactgggc acatcccatc gaaaacctgg tggcggtcgt tgatttagaa cagaaaaaaa
    12241 tcgttaagat tgaagaaggt ccggtagttc cggtgccaat gaccgcacgc ccatttgatg
    12301 gccgtgaccg cgttgctccg gcagttaagc ctatgcaaat cattgagcct gaaggtaaaa
    12361 attacaccat tactggcgat atgattcact ggcggaactg ggattttcac ctcagcatga
    12421 actctcgcgt cgggccgatg atctccaccg tgacttataa cgacaatggc accaaacgca
    12481 aagtcatgta cgaaggttct ctcggcggca tgattgtgcc ttacggtgat cctgatattg
    12541 gctggtactt taaagcgtat ctggactctg gtgactacgg tatgggcacg ctaacctcac
    12601 caattgctcg tggtaaagat gccccgtcta acgcagtgct ccttaatgaa accatcgccg
    12661 actacactgg cgtgccgatg gagatccctc gcgctatcgc ggtatttgaa cgttatgccg
    12721 ggcctgagta taagcatcag gaaatgggcc agcccaacgt cagtaccgaa cgccgggagt
    12781 tagtggtgcg ctggatcagt acagtgggta actatgacta catttttgac tggatcttcc
    12841 atgaaaacgg cactattggc attgatgccg gtgctacggg catcgaagcg gtgaaaggtg
    12901 ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga cacgcgctac ggcacgctta
    12961 tcgatcacaa tatcgtgggt actacacacc aacatattta taatttccgc ctcgatctgg
    13021 atgtagatgg cgagaataac agcctggtgg cgatggaccc agtggtaaaa ccgaatactg
    13081 ccggtggccc acgcaccagt accatgcaag ttaatcagta caacatcggc aatgaacagg
    13141 atgccgcgca gaagtttgat ccgggcacga ttcgtctgtt gagtaacccg aacaaagaga
    13201 accgcatggg caatccagtt tcctatcaaa ttattcctta tgcaggtggt actcacccgg
    13261 tagcaaaagg tgcccagttc gcgccggacg agtggatcta tcatcgttta agctttatgg
    13321 acaagcagct ttgggtaacg cgttatcatc ctggcgagcg tttcccggaa ggcaaatatc
    13381 cgaaccgttc tactcatgac accggtcttg gacaatacag taaagataac gagtcgctgg
    13441 acaacaccga cgccgttgtc tggatgacca ccggcaccac acatgtggcc cgcgccgaag
    13501 agtggccgat tatgccgacc gaatgggtac atactctgct taaaccatgg aacttctttg
    13561 acgaaacgcc aacgctaggg gcgctgaaga aagataagtg attgtttcag acaaaaaaac
    13621 ggcaccaggt gccgtttttt acttatgagc gaaccagatt aataccgtac acacaccgac
    13681 ttagtttcac accaaccgtc cagccagtcg ggaccaaaat cacggcccgt tcctgactgc
    13741 ttcatcccac caaacggtaa gttagcgtca attaaggtat ggctgtttac ccacaccgtc
    13801 cctgcctgta agcgatcgct atattccaga gcctgggaga gattttgcgt ccagacactg
    13861 gcagttaagc catattccgt gtcgtttgcc agttgtaacg cctcttctcc atccgctact
    13921 cgcaccaggt ttaccaccgg accaaacacc tcttcacgag ttaagcgcaa tttagcatcg
    13981 ggatttacca ccagcgttgg cgcaacataa tacccctctc cggctggtcc attcgacccg
    14041 cgaatcagct ctgcttgctg tgcctgcgca tcgtcgagga atgaacacac tttgtcgcag
    14101 tgcgcacgag aaaccaaagg gttaatctgt gcaacaggtg acatccccgg tcccacttgc
    14161 aacgatttta ccgcctgctc aaatccacta accagcgtgt caaacaacgg cgcttcaata
    14221 taaattcgcg aactggcggc gcatacttgc ccttgattca ggaagcttcc ggtcatcaag
    14281 ccttcaataa cccattgcgg atcagcatct tttaatacaa ttgccgggtt tttaccgccc
    14341 agttccagcg ttacacgcgt taagtgatca gcagcagttc tggcaatacc ttttcccgtc
    14401 gcggttgaac cggtaaaact gattttcgca acatgaggat gtgacgtcag ggccgcgccg
    14461 catacagcac ctgacccggt gacgacatta aaaacgccat cagggatacc agcctcgctg
    14521 gccagttccg ccacgcgcaa catcgtcagt ggcgtggttt ccgaaggctt aatcacgatt
    14581 gaacagcctg ctgccagtgc tggcatcacc ttccacatac caatcatcaa cggaaagttc
    14641 catggcacaa ttcccgccac tacgccaacc ggctctttac gcgtccaggc ctgataacgc
    14701 gccccctggg gtaagggaat cgacaagtcc agcgttttac ccgcgatttt ggtcgttaac
    14761 ccggcggtat aacgcatcca gttcagcgta cagcccactt caaaagcacg agaaatggca
    14821 attgacttgc cttgctccag ggtttccagt tgcgccagct cctcactgtg ctgctccacc
    14881 agatcagcaa aacgtagcag aatacgttca cgctctgcgg gtaatcgccc ggcccagcga
    14941 cgcgagacaa aggcccgcct gcgttatcta catccgcttc gttggcatca gcagtagacg
    15001 caatttcttg cccggtggcc ggatcaaaga tcgccaatcg tttttcactt tgtgcggggc
    15061 ctggacgacc atcaatataa agaccgtgtt gacgatcgag aaactgttgg acctggctta
    15121 atactgctac atgcggctct gtcatcactt ttccttatta tttacccagt gtgatttcag
    15181 tgtacgcagc gacaagaaaa gtcacttttt tcgctgtgac attcgctttg tcagctgtga
    15241 caagctccgc aaatcgtgac aataaccgct tatgtctcta tgcctgagaa gacttatttc
    15301 ctatagtcag gctcattagc ttcgtttact gcaacacaaa tgcaacaata aaaatacatt
    15361 tcacagagcg aaaacgtgcc atgaaccccg cagtggataa tgagtttcag caatggcttt
    15421 cccaaatcaa tcaggtatgc ggaaatttta ccggacacct gctgactgag cgttacacgg
    15481 gtgtactgga tacgcatttt gccaaaggac taaagctgag taccgtgaca accagcgggg
    15541 tgaatttatc ccgcacctgg caggaagtaa aaggcagcga cgatgcctgg ttttacaccg
    15601 tttttcagct tagtggtcag gcaataatgg agcaggatga gcgtcaggtg cagattggcg
    15661 ctggcgatat tacgttactc gatgcctcac gcccctgttc gctttactgg caggagtctt
    15721 ctaaacagat ttcattactt ttgccacgca ctctgctgga acaatatttt ccccatcaaa
    15781 aacctgtctg cgcagaaaga ctggacgctg acttacccat ggtgcaactc agtcatcgcc
    15841 tgttacagga gagcatgaat aatccggcac tttctgaaac agaaagtgaa gctgcgctac
    15901 aggcgatggt gtgtctgctg cgcccggtac ttcatcagcg ggaatctgtt caacctcgtc
    15961 gtgaacgtca gtttcaaaaa gtggttacgt tgatagacga taatattcgc gaagagatat
    16021 tacgcccgga gtggatagcc ggagagacag gtatgtcagt acgtagtttg taccgaatgt
    16081 ttgccgataa aggtttggta gtcgcgcaat atattcgtaa ccgtcgtctc gatttttgtg
    16141 cagatgcgat tcgccatgcc gcagataatg aaaaactggc aggcatctgc tttcattggg
    16201 gattttctga ccagagtcat ttttcaacgg tatttaagca acgctttggg atgacgccag
    16261 gcgagtatcg acgtaaattc cgctaactaa ttggcgttgt gtgtactgca acgccaatta
    16321 gttaaaacag atatttgact atgcattttt cagggcgaaa ggcaatttga caggagtttt
    16381 tgaatattca ccttgtttca ggaaaataga tcaatcgctt tctcctgtag cgttatcccg
    16441 atattgcggt tttgctatta aaattttcgc acccatttac cgtttatccc ttgtacatat
    16501 tcccccggtt tggcccgagc tacgagcttt tgcccggcga gtttcgcgat atcatctaca
    16561 gacacattat tttgctttgc cagttgttgg taactcgctt tacgttcggc attaatgtcg
    16621 gttactaatt tctctgtttc agcatccgtt ttcagcgcaa ccagataacc ataaaatgtt
    16681 tcacctaccc gcccctgagt tctggcttca tccagagtca atgccattac attgctgctt
    16741 accagcccaa caagaaacgc acaaaaaagt aatgttttct tcatcatcgc ctcagaaaag
    16801 atcgctacga gtttcaagca gctcttcgac atctttgtct gccttgatga tgatctcatg
    16861 ctcaatttta acgttcatat tgatagtgat cggttccttg ggcgcagcga cttcaatacg
    16921 cggcgtacag gcagccagca taaaagatga cgtcaacgca gccagtaaaa ttttcattgt
    16981 ttttcctcac actcttttcc ttgcggacag tcatttcccg gcagacgtgc gttctgctcc
    17041 aaccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa aatattctct
    17101 tcatgatggt aattaaggtt taccgtaccg cttttaccat caacgcgact ggtacctgta
    17161 atgttggcct gcatggttaa taaaccgaga ttatctaagt taatttttgt cgatgaccgg
    17221 ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cagtcatatt gtctttcacc
    17281 accgcatctg cggtgtcttt atccagccgc aaggtcattg gcccgctatt tgccagccaa
    17341 ccatctttca ctatccattt ttcgctgttt agccataacg gcaatgcacc actaaatgcc
    17401 ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga tgagagatta
    17461 ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat cagtacgtta
    17521 ccgcctaaca catcgacgct aacatcagtc aacagcaagg gttcttcttc agtccacgga
    17581 taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat cacttcggca
    17641 attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc aaaacggaaa
    17701 ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact accgcctttc
    17761 aacacgccgt gtcctcccgc gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga
    17821 gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccag cggctggaat
    17881 acagtcagtg attgttttgg ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga
    17941 ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa gaggaaatac
    18001 gtcggatcgc gcccatcgac gctaaatttt aaggttgatg gcggtaacac actgccgcca
    18061 gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg
    18121 tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac cgtcatcgta
    18181 ccgtattgta actggtcgaa accggtggag aggtcggtca gcgtaatcgt gctgtcatgc
    18241 cattcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact gccttttccc
    18301 cagtagcgcc actgccagcg gcccgcgtca gggagaaaat cattcgccag cccatccata
    18361 tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg caaacgtccg
    18421 tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc atcgatgtcc
    18481 agcgaatcga tgactcttcc cttcgaacgt aataacgcgc ctggctcaaa ggccagcgtt
    18541 gggtcagtca gacttccact tagctgcgcg ggtaagcggg catataaaat gaggtccgcc
    18601 tgtttcgctt caccggtcag ttgcagaggc agctggctgt tatccatgct taattttcct
    18661 gggccaaaat ttagcaccgc gttgccttta cccgcttgcc cctgggtcag cacactcagt
    18721 cgtccgctga tcagagcgtt ctcaagccct gcctgccagt tgtcgacttt gacacccagt
    18781 cggccactca aaggaaaacc tgcatacggc caactccagc gaccatcgct tacggtcaat
    18841 tgttgacgag taatttgcca cggcaaatcg agcaacggat cgccgttatc ccgtgccagc
    18901 acaatcaatt gcccgctatt ttcctgccag tccagctctg catccaccag tgacggttcc
    18961 tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag gaagtccatc cggcacgagc
    19021 ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc gacgacatcc
    19081 agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac tttttcgccc
    19141 tgataacgca gttgctggat atcagaggtt aatgcgagag agagttttcc ctgccattcc
    19201 tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc
    19261 cactgcgcga gggtttttgg agcagctggc gattgctcca tctgcggcaa tttcgccaga
    19321 caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg
    19381 ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg
    19441 attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt
    19501 agccagatgc ccgccagagt gggaacccac agcccgagcg tcatcaacag cgtcaacggc
    19561 acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt
    19621 taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc
    19681 gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc
    19741 atagctttca attaaatttg aaattttgta aaatattttt agtagcttaa atgtgattca
    19801 acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga
    19861 agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc
    19921 tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg
    19981 atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc
    20041 tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg gggctgaaag
    20101 tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc ggtatgatga
    20161 tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc
    20221 tggaaggtct gaccggattt actatgtatg gcaaaacggc aggcgttatc ggtaccggta
    20281 aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg
    20341 atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc
    20401 tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc
    20461 tgttgaacga agccgccttc gatcagatga aaaatggcgt gatgatcgtc aataccagtc
    20521 gcggtgcatt gattgattct caggcagcaa ttgaagcgct gaaaaatcag aaaattggtt
    20581 cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagac aaatccaacg
    20641 acgtgatcca ggatgacgtg ttccgtcgtc tgtctgcctg ccacaacgtg ctgtttaccg
    20701 ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa
    20761 acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa tcttgccgct
    20821 cccctgcaat ccaggggagc tgattcagat aatccccaat gacctttcat tctctattct
    20881 taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaac tagccgcgtt
    20941 tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc
    21001 agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag
    21061 cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg
    21121 taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc
    21181 aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca ccattagcga
    21241 aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc tggcgaccgc
    21301 gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc cacagctccc
    21361 ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca tcccaatcgc
    21421 cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac aatttgcttc
    21481 tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag tcgcctgctg
    21541 aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc aaaacgctgc
    21601 tcgcatctct tcttcctctg atcttcaagc caaacgacac cgccataaat aataggcagc
    21661 acagagggcg gcgtcgagag ctgtcctgcg cgttgccccg catttttact tttttatggc
    21721 tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg gacacaaaag
    21781 cgaaaatgca gaagaaagcc atttgctaaa attgaaggat tactactggg cgcgcagcaa
    21841 tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat tgacggtaat
    21901 ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta ccctattacc
    21961 cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt gaagaacgtt
    22021 tggggagaca caccacgcgt ggttgaaatg cagtcggaag cgggtgctat cgctaccgtg
    22081 catggcgctt tgcagacggg tgccctttca acatcgttta cgtcatcgca gggtttgctg
    22141 ctgatgatcc cgacgctgta caaactggca ggcgaactca caccgtttgt cctgcatgta
    22201 gcggcacgta ccgttgccac acatgcactc tctatttttg gcgatcattc cgacgtcatg
    22261 gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcaa acgtccagga agcgcaagac
    22321 tttgctctca tttcgcacat cgcgacgctg aaaagccgcg tgccatttat tcatttcttt
    22381 gatggtttcc gcacgtccca cgaaatcaac aaaattgtcc cgctggccga tgacacgatt
    22441 cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcact caacccggaa
    22501 catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc tcgcgaagcc
    22561 accaacccat ggtacgacgc ggtctatgac catgttgaac aggcgatgaa tgatttctct
    22621 gccgcgacag gtcgtcagta tcagccgttt gaatattacg ggcatccgca agcggaacgg
    22681 gtgattatcc tgatgggctc tgccattggc acctgtgaag aagtggttga tgaattgcta
    22741 acccgtggcg aaaaagtcgg cgtgctgaaa gttcgcctgt accgcccctt ctcagccaaa
    22801 cacttgctac aaactctacc gggatccgta cgtagcgtgg cggtactgga cagaaccaaa
    22861 gaacccggtg cccaggcaga accgctctat ctggatgtga tgaccgcact ggcagaagcc
    22921 tttaataatg gcaagcgcga aactctgccc cgtgtcattg gtgggcgcta tggtctttca
    22981 tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa cgcggctaaa
    23041 ccgaaagcgc gctttacggt tggtatttac gatgatgtaa ccaatctgtc cctgccgttg
    23101 ccggaaaaca cccagccaaa ctcggcgaaa cttgaagcct tgttttatgg ccttggtagt
    23161 gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc cacgccgtgg
    23221 tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac ggtttctcac
    23281 cttcgagtga gcgaacagcc gattcgttcc gcttatctca tttcccaggc tgattttgtt
    23341 ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt aaaacctggc
    23401 ggcattttcc tgctcaacac accgtacagc gcagatgaag tgtggtcgcg cttgccgcaa
    23461 gaagttcagg ccgtgttaaa ccagaaaaaa gcgcgcttct atgtgattaa cgcggcgaaa
    23521 atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat ggcttttttc
    23581 catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg tgcgattgcc
    23641 aaaagttaca gtagcaaagg ccaggatctg gtggaacgca attggcaggc tctggcgctg
    23701 gcgcgtgaat ccgtagaaga agttccgttg caaccggtaa atccgcacag cgccaatcga
    23761 ccgccagtgg tttccgatgc cgctcctgat ttcgtgaaaa ccgtaaccgc tgcgatgctc
    23821 gccgggcttg gtgacgccct ccccgtttcg gcgctgccgc cagacggctc ctggccgatg
    23881 ggcactacgc gctgggaaaa acgtaatatt gccgaagaga tccccacctg gaaagaggaa
    23941 ctctgtaccc aatgtaacca ctgcgttgcc gcttgcccac actcagctat tcgcgcaaaa
    24001 gtggtgccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct ggatgtgaaa
    24061 tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg caccggaaga ttgcaccggt
    24121 tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga gattaaagcc
    24181 atcaatatga tgtctcgcct ggaacatgtc gaagaagaga aaatcaatta cgatttcttc
    24241 ctcaacctgc cagaaatcga ccgtagcaaa ctgggacgta ttgatattcg tacatcgcag
    24301 ctgattacac cgctgtttga atattcaggt gcttgctccg gttgtggcga gacgccgtat
    24361 attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaacgc caccggctgt
    24421 tcttcaattt atggcggtaa cctgccctct acaccgtata ccaccgatgc caacggtcgt
    24481 gggccggcat gggcgaactc tctatttgaa gataatgccg aatttggcct tggtttccgc
    24541 ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt tgccgataaa
    24601 atcccggcgg aattactgac ggcgttgaaa tcagacgcta cgccagaggt tcgtcgagaa
    24661 caggtcgcag ctttacgcca gcaactcaac gatgttgccg aagcacatga actgctgcgt
    24721 gacgcagatg cactggtgga aaaatcaatc tggctgattg gtggcgacgg ctgggcttac
    24781 gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt caacattctg
    24841 gtgctggata cgcaatgcta ttccaacacc ggtggtcagg cgtcgaaagc gacaccgctg
    24901 ggtgcagtaa ctaagttcgg cgagcacggc aaacgtaaag cgcgtaaaga tcttggcgtc
    24961 agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc gcagctgaac
    25021 cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct gatcattgct
    25081 tatagcccgt gtgaagagca tggttacgat ctggcactca gccatgacca gatgcgccaa
    25141 ctcacagcta ccggcttctg gccgctatat cgcttcgatc ctcgtcgtgc cgatgaaggc
    25201 aaactgccgc tggcgctgga ttcacgcccg ccgtcagaag cactggaaga aacgttactt
    25261 cacgagcaac gtttccgtcg tctgaattcg cagcagccag aagtggcaga acagttatgg
    25321 aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat ggccggaaaa
    25381 gcggaaaaaa gcaacaccga ttaaatgctc tggataagga ttatccaatt ctaaaaaaaa
    25441 agcccggacg actgttcggg cttgtctttt tatatgttgg aaaatcagtg gcaatgcaat
    25501 ggcccaacag aaaataaaat ctgtgacaat aaaggcatat aacccgcgca gaataacgta
    25561 tagaaaatta aaagtataat ttttattttg tatgtattat cttcgataca ttcaccagac
    25621 ttattatcat ttcaggagaa ttatttgatt aaggttttac ttaaggcgta acaaatgata
    25681 tttatcggct aactgaactt ctcctttagg atgttttcac tccccaaaat ggggataaaa
    25741 ggcaaataaa ataactaaag gatttattca atgaaaagca aagtactggc acttttaatt
    25801 cctgccctgc tcgccgcagg tgctgcacat gcagccgaag tttataataa agacggcaac
    25861 aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa ttcagcgaaa
    25921 gatggcgacc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat taacgatcaa
    25981 cttactggct acggtcaatg ggaatacaat attcaggcaa acaacactga atcttcaaaa
    26041 aaccagtcat ggacccgtct ggcatttgcc ggactgaaat ttgcagatta cggttctttc
    26101 gattacggac gtaattatgg cgtaatgtac gacatcgaag gctggaccga tatgctgcct
    26161 gaatttggcg gtgactctta taccaatgca gacaacttta tgactggtcg agccaatggc
    26221 gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg
    26281 cagtatcaag gtaacaacga aggtgtcagt aatggtcagg aaggcaccaa caacggacgt
    26341 gatgttcgcc atgaaaacgg tgacggctgg ggtctttcca caacatatga tttaggcatg
    26401 ggctttagcg ctggtgcggc atacacctct tctgaccgca ccaatgacca ggttaaccat
    26461 actgcggcgg gtggtgataa agcagacgcg tggactgctg ggctaaaata cgatgctaac
    26521 aatatttacc tggcaaccat gtattcagaa acgcgtaata tgaccccgtt tggcgacagc
    26581 gattatgctg ttgcaaacaa aacccagaac tttgaagtca ctgcacagta ccagtttgat
    26641 tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct gcacgctgcc
    26701 ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata cgccgatgtt
    26761 ggcgcgactt actatttcaa taaaaacatg tccacttatg ttgactataa aatcaacctg
    26821 ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga tattgtcgat
    26881 ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc
    26941 ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca caaaaagtgt
    27001 catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac agaacgattc
    27061 ttgtccctat cgatatttcc gattcagaat taactcaacg cgtgattagc cacgttgagg
    27121 cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct tcactgccct
    27181 actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat gacctgaaag
    27241 cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca accgacagag
    27301 tgcatgtcca tgttgaggaa ggctcgccaa aagaccgcat tctggaattg gcgaagaaga
    27361 tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact tatctgctcg
    27421 gttctaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac
    27481 actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg ctgacatttt
    27541 cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta tgctgggcgt
    27601 tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg
    27661 caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaagtcaga gtaaaaaccg
    27721 caactatctg acgaacaata acttttatgt ttttcaatat gttaggcagg attaacaact
    27781 acaataaagc acaataatct acactttttt tgccccattt ttgccccttt tgcggccttt
    27841 tgtcccattt ttgccacagt aaaaatctat aactaatcaa tctggcgccc atgatttctt
    27901 tccaatagaa tcagttccgg ctggaaatca ttagatactc gcactatcga aaagcttacc
    27961 agccaaccgc ggcacgttct tgcatacgag gtgccgcgtt ttacatgccg taccgagaat
    28021 taatttcatt ttacgaaaag ttaacccgcc attgctacct taatttttta aaacgaagtt
    28081 acctctgtca gggtcaaaag cacaattatc tcttcctaca atcattgaaa ctgcgattga
    28141 ttccctgctc aaaggatgag ccgcccttgt tttaacaaga tgtattaatg ccattttatc
    28201 atacaacata caaacatttg aattcattga attcctcata aataccccat cttctggttc
    28261 acataatgta acagggcatg ttcgtaagct ctctggcact tgcaattgtg catgctccac
    28321 attaaatgca catacttcaa ttttatctaa caactcctct aactggtagt tagaaagcaa
    28381 atccatattt tcgagcatct caatttcacg cagtactgta acttcacggc ttgtcaaact
    28441 accatgatta agtgtacgtt caatatgttc tatatattga aggccaatct ggatattatc
    28501 attcgataaa ccatcctggt taagacttcc cataaaatat ccatttacct gcataggtcg
    28561 tattgacacc atcaactcac ccagtcttat ttgagcttct ccacgaatag tctgattacg
    28621 tatctcagac aaggtttctt gaggaatgtc agacaaaaaa ctaaccccag gtaaaatata
    28681 aggcgttaaa tctacaggca ttcctctccc tccattttaa taacttaatt ttgctttagt
    28741 aaaaaataaa agtacataaa actacattta tatcattaat tatcacttac aacaaaaagc
    28801 ttctcttttt gtatcaaagt gacattcatc ttttctcata atcattgatt ctgttatagg
    28861 ttctcgactc agaggatgag ctccaccagt ttcaacaagt tgcactaacg cgtccttatc
    28921 atatagagag catatctcag cacctcgtga gtttctcatg a
//
LOCUS       DAZUFB010000056        28255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZUFB010000056 DAZUFB010000000
VERSION     DAZUFB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.056"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..94
                     /gene="tnaC"
                     /locus_tag="ACHY3M_002749"
     CDS             20..94
                     /gene="tnaC"
                     /locus_tag="ACHY3M_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFS4317639.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            315..1730
                     /gene="tnaA"
                     /locus_tag="ACHY3M_002750"
     CDS             315..1730
                     /gene="tnaA"
                     /locus_tag="ACHY3M_002750"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFS4317640.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1821..3068
                     /gene="tnaB"
                     /locus_tag="ACHY3M_002751"
     CDS             1821..3068
                     /gene="tnaB"
                     /locus_tag="ACHY3M_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFS4317641.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3200..4375
                     /gene="mdtL"
                     /locus_tag="ACHY3M_002752"
     CDS             3200..4375
                     /gene="mdtL"
                     /locus_tag="ACHY3M_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFS4317642.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4350..5309
                     /gene="yidZ"
                     /locus_tag="ACHY3M_002753"
     CDS             4350..5309
                     /gene="yidZ"
                     /locus_tag="ACHY3M_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFS4317643.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5466..6215
                     /gene="yieE"
                     /locus_tag="ACHY3M_002754"
     CDS             5466..6215
                     /gene="yieE"
                     /locus_tag="ACHY3M_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFS4317644.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6237..6803
                     /gene="yieF"
                     /locus_tag="ACHY3M_002755"
                     /gene_synonym="chrR"
     CDS             6237..6803
                     /gene="yieF"
                     /locus_tag="ACHY3M_002755"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFS4317645.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQLRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6857..8194)
                     /gene="adeP"
                     /locus_tag="ACHY3M_002756"
     CDS             complement(6857..8194)
                     /gene="adeP"
                     /locus_tag="ACHY3M_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFS4317646.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8361..9026
                     /gene="yieH"
                     /locus_tag="ACHY3M_002757"
     CDS             8361..9026
                     /gene="yieH"
                     /locus_tag="ACHY3M_002757"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFS4317647.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9093..9566
                     /locus_tag="ACHY3M_002758"
     CDS             9093..9566
                     /locus_tag="ACHY3M_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317648.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9615..10199
                     /gene="cbrC"
                     /locus_tag="ACHY3M_002759"
     CDS             9615..10199
                     /gene="cbrC"
                     /locus_tag="ACHY3M_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFS4317649.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFTGSFQDDASIEGVEFEYDEEEFAGIKNTYPDEM
                     LKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIR
                     NSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10261..10983)
                     /gene="yieK"
                     /locus_tag="ACHY3M_002760"
     CDS             complement(10261..10983)
                     /gene="yieK"
                     /locus_tag="ACHY3M_002760"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4317650.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10998..12167)
                     /gene="yieL"
                     /locus_tag="ACHY3M_002761"
     CDS             complement(10998..12167)
                     /gene="yieL"
                     /locus_tag="ACHY3M_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4317651.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12194..13810)
                     /gene="bglH"
                     /locus_tag="ACHY3M_002762"
     CDS             complement(12194..13810)
                     /gene="bglH"
                     /locus_tag="ACHY3M_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFS4317652.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13896..15290)
                     /gene="bglB"
                     /locus_tag="ACHY3M_002763"
     CDS             complement(13896..15290)
                     /gene="bglB"
                     /locus_tag="ACHY3M_002763"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFS4317653.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSQLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15309..17185)
                     /gene="bglF"
                     /locus_tag="ACHY3M_002764"
                     /pseudo
     CDS             complement(15309..17185)
                     /gene="bglF"
                     /locus_tag="ACHY3M_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(17319..18155)
                     /gene="bglG"
                     /locus_tag="ACHY3M_002765"
     CDS             complement(17319..18155)
                     /gene="bglG"
                     /locus_tag="ACHY3M_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFS4317654.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18441..19166)
                     /gene="phoU"
                     /locus_tag="ACHY3M_002766"
     CDS             complement(18441..19166)
                     /gene="phoU"
                     /locus_tag="ACHY3M_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4317655.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19181..19954)
                     /gene="pstB"
                     /locus_tag="ACHY3M_002767"
     CDS             complement(19181..19954)
                     /gene="pstB"
                     /locus_tag="ACHY3M_002767"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4317656.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20045..20935)
                     /gene="pstA"
                     /locus_tag="ACHY3M_002768"
     CDS             complement(20045..20935)
                     /gene="pstA"
                     /locus_tag="ACHY3M_002768"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4317657.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20935..21894)
                     /gene="pstC"
                     /locus_tag="ACHY3M_002769"
     CDS             complement(20935..21894)
                     /gene="pstC"
                     /locus_tag="ACHY3M_002769"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFS4317658.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21981..23021)
                     /gene="pstS"
                     /locus_tag="ACHY3M_002770"
     CDS             complement(21981..23021)
                     /gene="pstS"
                     /locus_tag="ACHY3M_002770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4317659.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23269..24341)
                     /gene="lpfD-O113"
                     /locus_tag="ACHY3M_002771"
                     /pseudo
     CDS             complement(23269..24341)
                     /gene="lpfD-O113"
                     /locus_tag="ACHY3M_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system.; frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
     gene            complement(24352..26874)
                     /gene="lpfC-O113"
                     /locus_tag="ACHY3M_002772"
     CDS             complement(24352..26874)
                     /gene="lpfC-O113"
                     /locus_tag="ACHY3M_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFS4317660.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26899..27630)
                     /gene="lpfB-O113"
                     /locus_tag="ACHY3M_002773"
     CDS             complement(26899..27630)
                     /gene="lpfB-O113"
                     /locus_tag="ACHY3M_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFS4317661.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(27678..28250)
                     /gene="lpfA-O113"
                     /locus_tag="ACHY3M_002774"
     CDS             complement(27678..28250)
                     /gene="lpfA-O113"
                     /locus_tag="ACHY3M_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFS4317662.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         7211 a         7080 c         6596 g         7368 t
ORIGIN      
        1 tttcgaggat aagtgcatta tgaatatctt acatatatgt gtgacctcaa aatggttcaa
       61 tatcgacaat aagattgtcg atcaccgccc ttaatttacc cttctgtagc catcaccaga
      121 gccaaaccga ttagattcaa tgtgaacgat tagtttgcta tatcttaatt ttgccttttg
      181 caaaggccat ctctcgttta tttacttatt ttagtaaatg atggtgcttg tatatataca
      241 tggcgaatta atggatattg cagatgtaat attcacaggg atcactgtaa ttaaaataaa
      301 tgaaggatta tgtaatggaa aactttaaac atctccctga accgttccgc attcgtgtta
      361 ttgagccagt aaaacgtacc actcgcgctt atcgtgaaga ggcaattatt aaatccggta
      421 tgaacccatt cctgctggat agcgaagatg tgtttatcga tttactgacc gacagcggca
      481 ccggggcggt gacgcagagt atgcaggccg cgatgatgcg cggcgacgaa gcctacagcg
      541 gcagccgcag ctactatgcg ttagccgagt cagtgaaaaa tatctttggt tatcaataca
      601 ccattccgac tcaccagggc cgtggcgcag agcaaattta tattccggta ctgattaaaa
      661 aacgcgagca ggaaaaaggc ctggatcgca gcaaaatggt ggcattctct aactatttct
      721 ttgataccac gcagggccat agccagatta acggctgtac cgtgcgtaac gtctatatca
      781 aagaagcctt cgatactggc gtgcgttacg actttaaagg taactttgac cttgagggat
      841 tagaacgcgg tattgaagaa gttggcccga ataacgtgcc gtatatcgtt gcaaccatca
      901 ccagtaactc tgcaggtggt cagccggttt cactggcaaa cttaaaagcg atgtacagca
      961 tcgcgaagaa atacgatatt ccggtggtaa tggactccgc acgctttgct gaaaacgcct
     1021 atttcatcaa gcagcgtgaa gcagaataca aagactggac catcgagcag atcacccgcg
     1081 aaacctacaa atatgccgat atgctggcga tgtccgccaa gaaagatgcg atggtaccga
     1141 tgggcggctt gctgtgcatg aaagacgaca gcttctttga tgtgtacacc gagtgcagaa
     1201 ccctttgcgt ggtgcaggaa gggttcccga catatggcgg cctggaaggc ggcgcgatgg
     1261 agcgtctggc ggtaggtctg tatgacggca tgagtctcga ctggctggct tatcgtatcg
     1321 cgcaggtaca gtatctggtc gatggtctgg aagagattgg cgttgtctgc cagcaggcgg
     1381 gcggtcacgc ggcattcgtt gatgccggta aactgctgcc gcatattccg gcagaccagt
     1441 tcccggcaca ggcgctggcg tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa
     1501 ttggctcttt cctgttaggc cgcgatccga aaaccggtaa acaactgcca tgcccggctg
     1561 aactgctgcg tttaaccatt ccgcgcgcaa catatactca aacacatatg gacttcatta
     1621 ttgaagcctt taaacatgtg aaagagaacg cggcgaatat taaaggatta acctttacct
     1681 acgaaccaaa agtattgcgt cacttcaccg caaaactgaa agaagtttaa ttaatactac
     1741 agagtggcta taaggatgtt agccactctc ttaccctaca tcctcaataa caaaaatagc
     1801 cttcctctaa aggtggcatc atgactgatc aagctgaaaa aaagcactct gcattttggg
     1861 gtgttatggt tatagcaggt acagtaattg gtggaggtat gtttgcttta cctgttgatc
     1921 ttgccggtgc ctggtttttc tggggtgcct ttatccttat cattgcctgg ttttcaatgc
     1981 ttcattccgg gttattgtta ttagaagcaa atttaaatta tcctgtcggc tccagtttta
     2041 acaccatcac caaagattta atcggtaaca cctggaacat tatcagcggt attaccgttg
     2101 ccttcgttct ctatatcctc acttatgcct atatctctgc taatggtgcg atcattagtg
     2161 aaacgatatc aatgaatttg ggctatcacg ctaatccacg tattgtcggg atctgcacag
     2221 ccattttcgt tgccagcgta ttgtggataa gttcgttagc cgccagtcgc attacctcat
     2281 tgttcctcgg gctgaagatt atctcctttg tgatcgtgtt tggttctttc ttcttccagg
     2341 tcgattactc catcctgcgc gatgccacca gcaccactgc gggaacatct tacttcccgt
     2401 atatctttat ggctttgccg gtgtgtctgg cgtcatttgg tttccacggc aatattccca
     2461 gcctgattat ttgctatgga aaacgcaaag ataagttaat caaaagcgtg gtatttggtt
     2521 cgctgctggc gctggtgatt tatctcttct ggctctattg cacgatgggg aatattccgc
     2581 gcgaaagctt taaggcgata atctcctcag gcggcaacgt tgattcgctg gtgaaatcgt
     2641 tcctcggcac caaacagcac ggcattatcg agttttgcct gctggtgttc tctaacttag
     2701 ctgttgccag ttcgttcttt ggtgtcacgc tggggttgtt cgattatctg gcggacctgt
     2761 ttaagattga taactcccac ggcgggcgtt tcaaaaccgt gctgttaacc ttcctgccac
     2821 ctgcgttgtt gtatctgatc ttcccgaacg gctttattta cgggatcggc ggtgccgggc
     2881 tgtgcgccac catctgggcg gtcattattc ccgcagtgct tgcaatcaaa gctcgcaaga
     2941 agtttcccaa tcagatgttc acggtctggg gcggcaatct tattccggcg attgtcattc
     3001 tctttggtat aaccgtgatt ttgtgctggt tcggcaacgt ctttaacgtg ttacctaaat
     3061 ttggctaaat ccttcaagaa gccagccatt cgctggcttc ttgcctctca ggaaatcact
     3121 tatgtccaaa tggcaactcg cctgattctc cttcaccacg tatgctttgc gtcaccttac
     3181 tatcaggacg ctttagccca tgtcccgctt tttaatttgt agttttgccc tggttttact
     3241 ttatcccgcc gggattgata tgtacctcgt tggtttaccg cgcatcgccg ccgatctcaa
     3301 tgccagcgaa gcgcagttgc atattgcgtt ctccgtttat ctggcgggca tggcagctgc
     3361 gatgttattt gccggtaaag tggccgatcg ttcagggaga aagcctgtcg ccatccccgg
     3421 cgcggcgcta tttattatcg cttcggtgtt ctgttcactg gctgaaacca gcacgttatt
     3481 tcttgcaggg cgctttctac aggggttggg cgcaggctgt tgttacgtgg tggcgttcgc
     3541 cattttgcgc gacacgctgg atgatcgacg tcgggctaaa gtgctgtcgt tactcaacgg
     3601 tattacctgc atcattccgg tgttagcgcc ggtgctcgga cacctgatta tgcttaaatt
     3661 cccttggcag agtctgttct ggacgatggc aatgatgggc atcgcggtac tgatgttatc
     3721 tttgtttatt ttaaaagaaa cgcgcccagc gtcccccgct gcttcggaca aaccacgaga
     3781 aaatagcgag tcgctgctta atcggttttt cctcagccgt gttgttatca ccaccctcag
     3841 cgtttcggtg atcctcactt tcgtcaatac atcgccggta ttgctgatgg aaatcatggg
     3901 ttttgagcgc ggagaatacg ccaccattat ggcgttgacc gctggcgtca gcatgaccgt
     3961 ttcattctcc acgccatttg cgctgggaat ttttaagcca cgtacgttga tgatcacctc
     4021 gcaggtgtta ttccttgcgg cggggatcac ccttgccgtt tcaccttccc atgcggtttc
     4081 tctgtttggt atcacgctga tttgcgccgg tttctcggta ggttttggcg tagcgatgag
     4141 tcaggcgtta ggaccatttt cattacgcgc gggcgtagcc agctcgacct taggtattgc
     4201 gcaggtttgc ggttcgtcac tgtggatttg gctggcagcg gtggttggta tcggcgcatg
     4261 gaatatgctg atcgggattc tgattgcctg tagcatagtg agcctgttac tgattatgtt
     4321 cgtcgcgcct ggacgccccg ttgccgctca tgaagaaatc catcaccacg cttgatctca
     4381 atctgctgct ctgtctgcaa ctgctgatgc aggagcgcag cgtgaccaaa gcggcgaagc
     4441 ggatgaacgt gaccccttca gcggtgagta agtcgctggc aaagttaaga gcgtggtttg
     4501 acgacccgct ctttgtgaac tcaccgctgg ggctgtcgcc cacaccgcta atggtcagca
     4561 tggagcaaaa tctggcggaa tggatgcaaa tgagcaatct gctgctggat aaaccgcacc
     4621 accagacacc gcgcggcctg aagtttgagt tggcggcgga atcgccgctg atgatgatca
     4681 tgcttaatgc gctgtcgaaa cggatctacc aacgttaccc gcaggcgacc atcaaactac
     4741 gtaactggga ttacgattcc ttagatgcca ttactcgtgg tgaagtggat attggttttt
     4801 ccggtcgcga aagccatcct cgctcgcggg agctgttaag ctcgctaccg ttagccattg
     4861 attatgaagt gctgtttagt gatgtaccct gcgtctggtt acgcaaagat catccggcac
     4921 tgcatgaaac gtggaatctg gacacctttt tacgttatcc gcatatcagc atttgctggg
     4981 aacagagcga cacctgggcg ctggacaatg tgttacagga gctggggcgc gaacgcacga
     5041 ttgctatgag cctgccggaa tttgagcagt cactgtttat ggcagcgcaa cccgacaatc
     5101 tgctactggc gaccgcgccg cgctactgtc agtactacaa tcaactccat caactgccgt
     5161 tggttgctct tcctctcccg tttgacgaaa gccagcaaaa aaagctggaa gttcctttta
     5221 ccctgctgtg gcataaacgg aacagccata atccgaagat cgtctggtta cgggaaacca
     5281 ttaaaaacct ttacgcgtcg atggcataac cgaatcgtat gaaacgcgac ccaatttcac
     5341 aataaaatgt aaaaaagttg taataagctt gtctgaatcg aacttttagc cgctttagtc
     5401 tgtccatcat tccagtaaat gattactctt atattcataa tggaccatta agcatggagc
     5461 gaaaaatggc gactcacttt gcccgaggga ttttaacgga aggacatctg atttctgttc
     5521 gtctcccctc ccagtgtcat caagaagccc gaaacattcc ccctcatcgt caaagccgtt
     5581 ttctggcgtc cagaggttta ctcgcagaac tgatgttcat gctgtatggc attggcgaat
     5641 tgccggaaat cgtcaccctg ccgaaaggta aaccggtttt cagtgataaa aatttgcctt
     5701 cgttttctat ttcctatgcc gggaatatgg ttggcgtggc gttaacaacc gaaggtgaat
     5761 gtggcctcga tatggaacta cagcgtgcga cgcgcgggtt tcatagccca cacgcgcccg
     5821 ataaccacac cttttccagc aatgaatcgc tatggatcag taaacaaaac gatcctaacg
     5881 aagcgcgggc gcagctcatc acgctgcgcc gaagcgtgct aaaactaacc ggtgatgttt
     5941 tgaatgacga tccgcgcgat ctgcagctgc tgcccattgc cggacgcctg aaatgtgctc
     6001 atgtaaatca tgtagaagcg ttatgcgacg cggaggacgt gctagtgtgg tccgtggcgg
     6061 tcacgcccgc gattgaaaag ctcagtgtct gggagttaga tggcaaacac agctggaaaa
     6121 gcctgccgga tattcacagc cgcgccaaca atcctaccag ccggatgatg cgttttgccc
     6181 agctctctac cgtgaagtct ttttcgccaa attgatagac aaccacagga gtcattatgt
     6241 ctgaaaaatt gcaggtggtt acgttactgg ggagcctgcg caaaggctca tttaatggca
     6301 tggttgcacg taccctgccg aaaattgctc cggcgagcat ggaagtcaat gcgttacctt
     6361 ccattgccga cattcccctg tatgacgctg acgtacagca ggaagaaggt tttccagcaa
     6421 cggttgaagc tctggcggaa cagctccgtc aggctgacgg tgtggtgatc gtcacgccgg
     6481 aatataacta ctcggtaccg ggtgggctga aaaatgccat cgactggctt tcccgcctgc
     6541 cggatcaacc gctggccggt aaaccggtgt tgattcagac cagctcaatg ggcgtgattg
     6601 gcggcgcgcg ctgtcagtat cacctgcgcc agattctggt tttcctcgat gcaatggtga
     6661 tgaacaagcc ggaatttatg ggcggcgtga ttcagaacaa agttgatccg caaaccggag
     6721 aagtgattga tcagggtacg ctggaccacc tgaccgggca attgaccgca tttggtgagt
     6781 ttattcagcg agttaagatc taaataaaaa acccgccagc aatcatgcat ggcgggtttt
     6841 taacgcgcta tcgattttag tgagcgtcga taaagacaat cttcaggata aacagcagcg
     6901 caacgatgat tacgcacggg cttagatcac gcagacgtcc ggtaccgatt ttcatcacgc
     6961 agtaggagat aaagcccagc gcgatacctt cggtaatcga gaagctgaac ggcatcatca
     7021 cggcggtaat aaacgccgga acagattcag taagatcctg ccagttcacg cgtgccagac
     7081 tggaagtcat cagcacgcca acgtaaatca gcgcaccagc tgcagcgtag tctggcacca
     7141 tccccgccag cggcgacaga aagataacca gcaggaacag cagaccaaca accactgccg
     7201 tcagaccggt acgaccgcca accgatacgc cggaagagga ctcaatataa gccgtaacgg
     7261 aagaagtacc gataaacgaa ccggtcacgg aagagatact gtcgacatac agcgcctgct
     7321 tcatgcgcgg gaatttaccc ttctcatccg ccaggcctgc tttatcggtc acgccaatca
     7381 gcgtaccgga ggagtcaaac aggttgacca gcatgaaaga gaaaatcacc cctgccagcc
     7441 cgaggttaaa cgacccggct aaatctacat gacccacaac tgtcattacg ctcggcggcg
     7501 cagaaacgat gccattgtag tgcacatcac ccagcatcca gcccagcagc gtcgtcacca
     7561 cgatagaaac cagcaccgct gcgtgaatgt tgcgcgaggc cagaatagca atgatgaaga
     7621 agccgaggat acccagaagt acgctgtgag aagtcagatt accgatgctc accagcgttt
     7681 ccgggttagc gacaatcaca cctgcgtttt tcagccccat catgccaatg aacagaccga
     7741 taccgctggt aatacccaca cgcagactca caggaatgtt ggcaatcatc cagtagcgaa
     7801 cgcggaaaat cgtcagtaac agcagaccta tcgcgcccca gaagattgcg cccatcccga
     7861 cctgccacgg caagcccatc gcctgtacaa cgacaaaagc gaagaacgca ttcaggccca
     7921 tagcgggtgc cagtgcaact ggcaggttag caaacagtcc catcataata ctgccgaatg
     7981 cagcgatcag acaggtagtg acgaagacgg cgctggtatc catgccagca acgccaagaa
     8041 tttgcgggtt aacaaaaacg atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca
     8101 cttcggtccg tgccgtcgtg ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct
     8161 ggccagaagc ctgggttgtg tgttgttgac tcattatcta tttccgaaca aggagggaaa
     8221 atccgtcgct atcgtatacc aaaatgagac aatatggacg gctgaagata actttttttc
     8281 attgtatttg cttatacggc aacgattgcg tcatgcgaat tggcatgacg aaaacgttaa
     8341 actaacgata aaggaaagac atgtcccaga tagaagcggt atttttcgac tgcgacggta
     8401 cgctggtcga cagtgaagtc atttgctccc gcgcatatgt aacgatgttt caggaatttg
     8461 gtattacgct cgatcctgaa gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa
     8521 ttatcgatat tgtttccctt gaacatggtg ttacgttagc gaaaacagaa gctgaacacg
     8581 tttaccgtgc agaagtcgct cggctgttcg attcagaact ggaagccatc gaaggggctg
     8641 gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt atctaacggc ccaaataaca
     8701 aaatgcagca ttctatgggc aagctgaata tgttgcacta cttcccggat aaactgttca
     8761 gcggctacga tattcagcgc tggaagccag acccggcgtt aatgttccat gcggcaaaag
     8821 cgatgaatgt aaatgtagaa aactgcattc tggttgatga ctcagttgcc ggtgcgcaat
     8881 ctggtatcga cgcaggtatg gaagtgttct acttctgcgc cgacccgcac aataagccga
     8941 tcgttcaccc gaaagtcacc acctttaccc atctttcgca gttaccggaa ctgtggaaag
     9001 cgcgtggttg ggatattacg gcatagttct tcacactccc ttcacttacc ccgcttaaat
     9061 tggcgctcaa aggtaagtaa agggagtttg atatgtctgt ttcacgtcgg gtaatacatc
     9121 acggacttta ttttgcggtt ttaggaccgt taattggtgt tctgtttctt gtcctctaca
     9181 tattcttcgc aaaagaaccg ctggttcttt tgcgaagaat acaggtactt ccattgtttc
     9241 tcttattgtc gattactacg ggagttattc ctgcattgtt aaccggcgtg atggtcgcct
     9301 gtctgccgga aaagatcggg tcacagaaaa attatcgttg tctggctggt ggcataggtg
     9361 gcgtcgttat caccgagatc tattgtgcag ttattgtaca tattaagggc atggcttcct
     9421 cggagttgta tgaaaacatt ctttctggtg acagtctcgt tgtccgcatc attcctgcat
     9481 tgctggcagg tgtggtgatg agcagaatca ttacccgtct gcccggattg gatatttcat
     9541 gtcctgaaac agactcttta agttaagcgg gatactttat ctttgggcta ctcaaaagca
     9601 gacaggatgt tactatgact caaaatatca ggccgttacc ccaattcaaa tatcatccca
     9661 agccactgga aacaggcgca tttgaacagg ataaaaccgt agagtgcgat tgctgtgaac
     9721 aacagacgtc agtttattac tcgggtccct tttattgcgt tgatgaagtt gaacatctct
     9781 gtccgtggtg tattgcggac ggttctgctg ctgaaaagtt tacaggtagt tttcaggatg
     9841 atgccagcat agaaggtgtt gaatttgagt atgatgaaga ggaatttgcc ggtattaaga
     9901 acacatatcc tgatgaaatg ctgaaagagt tggttgaacg cacgccaggt tatcatggat
     9961 ggcaacagga attctggctt gcgcattgtg gcgatttctg tgcttttatc ggctatgtgg
    10021 gctggaatga tataaaagat cgcctcgatg aatttgccaa ccttgaagaa gattgtgaga
    10081 atttcggtat tagaaattct gatctagcta aatgcctgca aaagggtggt gattgtcagg
    10141 gttatctctt ccgctgtctc cactgcggca agctgagact gtggggtgat ttttcgtagt
    10201 tatttaaata atgagaacag gccggagcgt aattcataca tccggcctta tttcttcagc
    10261 ttaatcgagc gctaattctg cggcagcggc tttatcagcg attaccatca gcgaaggatg
    10321 cagctgcaac acggaagctg gaacatcttc ggtaaccgga ccttgcagca catttttcag
    10381 tgcctgcgct ttacctgcgc cactgacaat tatcagcaag tttttcgccg ccatgatgct
    10441 tttcggcccc atcgtcacgt agctgtcagg cactaacgaa aagtcgccgc ccaactcacc
    10501 atgcgccaca atatcaacca tctctccctg gatcgggaac tccacggttt gctcatggaa
    10561 atgggtcgta ttcggcaaat tgccacaaaa atgaccatct gcacctaatc ccagcaccac
    10621 caaatccagg ccgccttcac gcgccagttt ctgatcatgc tcgcggtagt tatcaatagt
    10681 gagcttctgg atattctctt ctttgatccc cgcaggggtg aaaaacagat tacgcagatt
    10741 ggtaatcgtt acaccttctc cctctttgcc gcgaaaggga atttcatcga aattatagaa
    10801 atagcagtta tcataccagg gcttaccttt aaccagggtg gtgaggtatt catacatgcc
    10861 tttgggcgtg ctaccggcgg taattgccag gttaacacga cgcgtcttcg acatataacc
    10921 aagcagatgg tgtgccgcga cacggctcat ttcctggtaa tcttcggtaa tgattaattt
    10981 catcttagtg ccttatttta tttaaataat ttctgtacga aggctgcata ggcaggccgc
    11041 cagacatcca tttcatggtt caggccggga tattcctggt aatcaaactt aatttttttc
    11101 tgctcaagct cagttttcag cccggcgata tccttgccgg ttacgacatc tttatcacca
    11161 acgaccacag taaaattacg tagttgctgg ttaatagctg ccggatcgtt cagccgggcc
    11221 gcgacacctt catccggtac ggttgtcgtg gtaacaccac tgaatgtggc cagccagcca
    11281 aagctttcca gatgattcat tccggaaacc agcgcctggt acccgccttg tgaaagccct
    11341 gccagcgcgc gaccatcggc atctttacgg acattaaaac gcttgctaat cagcgggata
    11401 atatcgttca tcagttcgcg atctgccgct ttagcattca gcggataaaa gactttacgt
    11461 ctttcctgag gcacgaaatc ttcgggaata atgcccttcg catcggtttc ggtatccggg
    11521 atcaccacca gcatcggttt aattttccct tcagccagca ggttatccat gatttgcggg
    11581 atacgcccct gatcgatagc ggaacgtccg gtatcaccaa aaccgtgata gaaatagagc
    11641 actggcaaag gctcgcccat gccggtgtaa cctggcgggg tccagacata catctgacgt
    11701 tcagattgca atgcgttgga gtggtaagtt atggcgatca aatcaccatg cgcaacagaa
    11761 cgcgtatcca gataactgcc tggtaccaga atcatactgg agttaacctg gcgctgaggc
    11821 ttagtcattg cggtgcctgt atcaatgctg cgtacaccat caacattgaa aaaatactcg
    11881 tacaggttgc ctttcagaat aggtgtgcgc cacgaccaga ctcctgcttc gtctttggtc
    11941 atcgggtgaa tattgtccgg aaccggaaca cccaccacta cagagacatt ttttgcccca
    12001 ggggcaaagt agcgaaaggt cacgctgtta tcggcattga cctgagtcac atactgtttc
    12061 accgggatag tgggtgccgg gctggctggc atatccgctg ctatggccca ctgagcagaa
    12121 ataccgctgg caatagccag cgttaaagca gcaattttta tattcatcgt aatattcctt
    12181 tcattaagtt ccattaccac cagatttcag cctgggcacc gacagcgaac tggtcgtctt
    12241 tattgtcttc gaaggtgaag ccatccagtt cgttttccag ggctttgata tacgtggcgt
    12301 agaaacgaat ttccggacgc gaggtcaaca tactggtatt gactttaaag gtatggaaga
    12361 gcgtggtttt ataaccagac tcattaaatt tattactatt cgcatctttg ttttgctggg
    12421 tgaaatagcc cagttcaaca cctgtctggt tatattggtc ccaaatatag gccgggcgaa
    12481 caaccgcacg aatagattcg aaatcagagt gtgcgcctgt ttcgtagcta taaatatcgt
    12541 taccgaagga gtaaacaatc gcgttagcca caataaaatg atcgccgata taggcttcgc
    12601 cctgtgaagt cagacgaact gctgttccgc cggtgtgatc accataataa cgaccattaa
    12661 aggtggtaaa tggacttgcg ccagcataac ggccaaagtt actggcgata gagttattcg
    12721 caaccaggaa ggagaattcg ttgaagccac ctttatcaaa tttctgcgtt aaagatgtgc
    12781 caaacatcca ggtatctttc cagtcataat agccgttatt atcctgatta tctttttccg
    12841 atgcgctttc gtttgccgtg acataacgac cactcaccat taaggtcgct ttatcccata
    12901 acgggatatc tttatagcgt aaatcaatag tattggtatt aatctgctgt ttgttttgca
    12961 ggctgcgatc gtaatcatcg acatcttcgc gaaccagcgc gatatcaatt ttacccggac
    13021 cgactttcca gttttccaga cctacacccg ctgcggcatc agtacgctgc gttttccagt
    13081 ccagcatctg gatttcaatt ttcggcgcac cgtgtttacc cacccagaaa tcagcctctg
    13141 gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc ggagaactgc ataaagttct
    13201 cgccaccggc gttatcgcca aaccagccgg tagagtactg ctgaccaacg ttaccatcca
    13261 tcatcacaac ggcatcaacc cgtttgccgt tttcgttgta gacacgttgt tttaactgca
    13321 aatcaaacca gccggagtat tcgttaccaa agcggcccag agaaccaatc gcccatgatt
    13381 taggtgaacc atgagaggcg gtcccccaac cagaacggta atagccgttg taactaaagc
    13441 caatttcatc tttcacaaac ttactgaaat ctttcagagt catggaagaa taaatcgagc
    13501 ctgtctcact ggcatttttc tcttcatttt tgaccagtac agcatcaggt tttgcggcac
    13561 tactggtcgg gaacggattg gcggcatacc cttgatcatt cgtacttacg ctacgattca
    13621 ccgtcgctgg cgtatatttt ttcttctctt catctttata ctttttgagt tcgctttgcg
    13681 tttctcttaa cgctttttct aataactcaa ggcgatgttc caccgttaat gattcagcca
    13741 atgattgtgc agaaaaggct aacggtgcca ttaataagat ggcagaggta ataagatttc
    13801 gtctaaacat aatatccctt tatggtgcaa agaaagaatt aacgtatcgc atccagactg
    13861 ttctgaatgc gacgataatt aaggtgcttt attggttatt tttttaatga cagcccccgc
    13921 gttttgatca cttctgcata ccatccgaag cttttcttac gcgttcttgt gaggcttcct
    13981 tcgccattat catcacgatc cacataaata aagccgtagc gcttagacat ttgtgaatga
    14041 gatgcactga ctaaatcaat tggcccccaa ctggtgtacc ccataatatc cacaccatcg
    14101 gcaatcgctt catttacctg taccaggtga tcgtttaaat aggcaattcg ataatcgtcc
    14161 tgtatcgaac catccgcttc aacgctgtct tttgcgccta atccgttctc gacaataaat
    14221 aacggttttt gataacgatc ccaaagcgta tttaacagaa cccgtaatcc aaccgaatca
    14281 atttgccacc cccactctga acttttcaga tgcggattgg ggatcatatt cagtatgttg
    14341 ccctgcgcat ttttattaat gctttcgtcg tgggaaacac aaccagtcat gtaataacta
    14401 aaagagatga aatcgacggt atgttttaaa tcttctgcgt cactttcagt catctcaatg
    14461 gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg gatactggcc acgcgcctga
    14521 acatcaccaa agaacatcca gcgccggttc tcttccatgg cctgcaacat atcctgtggc
    14581 tggcaggtga gggggtaaac cagcccaccg agaagcatat tgccgatttt cgcttcgggg
    14641 agctggctat gacaggcttt aactgcccgc gcactggcaa ccagttgatg gtggatagcc
    14701 tgataaactt ccgcctcgcc actctcttct gccagcccca cgcccgtgaa tggctcgtgt
    14761 aacgacatgt tgatttcatt aaacgtcagc cataacgcca ctttatgttg gtagcgagta
    14821 aagaccgtgc gggcgtaatg ctcgaagtga tcgatgaccg ctcgattagc ccaaccgccg
    14881 tagtttttca ccagcccata tggcatttcg taatgggata acgttaccag cggcttgatc
    14941 cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg ctaaccccgc ttcattcggt
    15001 tcgacttcgt cgccctgagg gaaaattcgc gcccaggcaa tggaaatacg cagacaggtg
    15061 aagcccatct cggcaaataa cgcgatatct tccgggtaac ggtgataaaa atcgatggcg
    15121 acatctttga tattctcttt ccccaggatg cgcggttcca tttttcccat tacgccatga
    15181 ggctgtaaat ctgaggtcga gatccctttg ccatcttcct gccaggcacc ttccacctga
    15241 ttggcagctg ttgcgccacc ccaaagaaat gtttctggaa atgctttcat aattaactcc
    15301 ttttatcgtt agcgaatgat ggataacagc ggttcacctg cgcttatctg cgccgtgccg
    15361 tggggtaata cgtccgtaaa atcatcgcta ttactgatta ataccggcgt cgtcagatca
    15421 aatccggcct cgcgaatagc agggatatca aaagaaatca gccgatcgcc tgtattgacc
    15481 ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt ccagttttac ggtgtcgata
    15541 ccgacatgaa tcaggagctc cacaccatca tctgactcaa tgccaatggc gtgtaatgtg
    15601 gcgaacaacg aagcaattcg acccgcaacc ggagaacgca cttcaccaac cgagggcaga
    15661 atggcgatac ctttacccaa caggccactg gcaaacgtgg tatcagcgac gtgaatgagc
    15721 ggcacaatct ctcccgtcat cggtgaacag ataccgccct gctcaggtgg tgtaataacc
    15781 tctggtgttt tctcttgcgg ggcaccctgc gctggctgac gtttagcggt gatgaaatga
    15841 agcatcaccg taccgacaaa tgcgcaaccg atggcaatga caccgccaat aacgctggcc
    15901 tagacggtga aatcaattcc cgttgacggg atggtttgca tgaaggtgaa aatacttggc
    15961 aaaccaaagg agtacacttt cgtttgcgcg tagccaatga tggtggcccc aaagccccac
    16021 tgatacaggc gataacaaag gggtacttac gcggcagatt aacgccatat accgctggtt
    16081 cggtgatacc aaacaggccc gtcaacgccg ctgatcccgc caccactttt ttctgcgcat
    16141 cgcgttcgca gaggaagacg ccgagcgccg ccccgacctg cgccataatg gcgggcatta
    16201 acagcgggat catggtgtcg tagcccagca cggtgaagtt attgatacac agcggcacca
    16261 ggccccagtg cagtccgaac atgacgaaga tttgccagaa gccgcccatt accgcgcctg
    16321 caaatgcagg aaccgcctga taaagccaga gataaccggc ggcaatcagt tcgcttatcc
    16381 aggttgatag cggccccacc agcagaaagg tgacgggtgt gataaccatc agacatagca
    16441 atggtgtgaa gaaatttttg attgccgacg gtaaccacgc attaagtcgg cgttccagaa
    16501 tgctgcacaa ccaggcagaa aaaataatgg gaataaccga tgacgagtaa ttcaacaatg
    16561 tgaccggaat acccaggaaa tccagcccca gctcatccgc tttttgcccg ttctcgaaag
    16621 cagtcagaat taatggatgc actaacgctc caccaatcac catggcagta aatggattac
    16681 cgccgaagcg tttccccgcg gtgtatccca ggattatcgg gaagaaccaa aacaaggcat
    16741 cactggcgct gaataaaatt aaataagtac cactttgttc ggtcgtccac tgaaaagtga
    16801 gcgccagagc cagcatacct ttcaggatcc cggttgccgc catcaaaccg atcagaggcg
    16861 taaaaatacc tgaaataaca taaacaaagc ggtttagcag attacctttc tcatcatttt
    16921 ccggtgcctg ttgcgctttt tcgccaaggc ctgccacgct gttgaccgcc aggaagacat
    16981 cggccacatg gttacctatg accacctgaa actgaccacc gctttccacc accataataa
    17041 taccgggggt ctttttcagt acctcagctt gcgctttgct ttcatccttt aacttaaaac
    17101 gtaatcgcgt tgcgcaatgc atcagactca caatgttatc tgcgcccccg actcctgcga
    17161 ctatttttct ggctaactcc gtcataactt gccctctacc gctttgcggc aaaactccaa
    17221 aaaaaaacct gaaaaaaacg gcctgacgtg aatcaagcaa tttttttcag gttttgcccg
    17281 cttagtgcgg taacaatcct ttactcagta ataatatttc agtgttcttt gcgcacgcgc
    17341 tctatattta tggctaaaaa cataatctct gcgggtgaaa ttttacgttg atactgcaaa
    17401 ccaataaaaa tggcgatccg ttccgcacat tgccatgctt gcgggtaatt ttgttttact
    17461 gcttgttgta atgattcatc actatcgtta attgaagcat gttcaagaat acgccaggat
    17521 aaaaacttca gatgtgtaac cagtcgctga taactcaagc tttcttcctg gtaattaagg
    17581 ctgaactgaa attttattaa ttgcagcatt tcgcgcatta actgcgtgac acctgcaaca
    17641 tcctccatat ttccgctcat ttgggcactg accagatgca tggcaataaa gcccacttca
    17701 tctttcggta actgcacgcc caaccgttta tcaataatgg ttaacgcttc ttcccctagc
    17761 tggaactctt tcgggtaaag ccgctggata tcccacagca acgggttggg cagcaacacg
    17821 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
    17881 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa tacgatcaca ggttgccatc
    17941 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
    18001 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
    18061 ccaattcctc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
    18121 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
    18181 aaaacccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
    18241 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
    18301 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
    18361 aactttgtgt aattttagga atttataaaa ttatatataa caaatcccaa taattaagtt
    18421 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
    18481 tcatcaccgc ctacatgacg gaaatcctgc cccttcacgt agtagaagat aaactcacaa
    18541 atattctggc agcggtcgcc aatacgttcg atagaacgcg cgcagaacag cgcagtaagt
    18601 acgctcggaa tggtacgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
    18661 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
    18721 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
    18781 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
    18841 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
    18901 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
    18961 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
    19021 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
    19081 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
    19141 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
    19201 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
    19261 caatcaattc gcccaggtac ataaacgccg tgtggtcgga acaacgcgca gcctgctgca
    19321 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
    19381 tacgcccggt agagataggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
    19441 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
    19501 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
    19561 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaaag
    19621 cgatgttgtc gtagatggac atcggaaacg gcgtcggttt ctggaacacc atgcccactt
    19681 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
    19741 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
    19801 gcgtcgattt accgcagccg gacggcccga taaacgccgt tacctggttt ttagcgatat
    19861 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
    19921 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtgcctga
    19981 tgcacttcgc taatcaggct taccgtaggc cggatcaggc gttcacgccg catccggcaa
    20041 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagta
    20101 cgcacagggt aatgatcaat accccggccc aggccagttg ctgccattcc gcaaacggac
    20161 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
    20221 tccagaactg gttggagagc gcggtaaata gcagcggcgc ggtttcacca gcaatacggg
    20281 caatcgccag caagataccg gtcatgatcc cggacaccga cgctttcagc gtaatcgcgg
    20341 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
    20401 ccagtttcag catgttctcg gtggtgcgga taacaatcgg cacctgcaac agcgccagcg
    20461 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
    20521 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
    20581 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
    20641 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
    20701 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
    20761 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
    20821 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
    20881 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
    20941 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
    21001 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
    21061 cgcttccgca aattcgttcg ccagcgcaga ggtgatactg ttgcccggca tatacagcga
    21121 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
    21181 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
    21241 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
    21301 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
    21361 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
    21421 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
    21481 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
    21541 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
    21601 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
    21661 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
    21721 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
    21781 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
    21841 aattatgtcg ccctttttac ccggtgggtt aaaagcaggc ttggttgcag ccataagtta
    21901 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
    21961 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
    22021 ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta atccaggtcg
    22081 ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac ttctgtgcct
    22141 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
    22201 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
    22261 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
    22321 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
    22381 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
    22441 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
    22501 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
    22561 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
    22621 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
    22681 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
    22741 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
    22801 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
    22861 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
    22921 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcgctcat cgataaggtc
    22981 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
    23041 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
    23101 gtaaggaata tgacattttt atgatagaaa ggaattacca aataacttac tgattttaaa
    23161 tgtgaagatg aaaatcccac atgtcatctt tttgtcatga tgcatttaaa agtgataaag
    23221 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
    23281 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gatgggcgag ctccagtaag
    23341 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
    23401 acccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
    23461 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
    23521 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
    23581 aatagtgacc gttttagtaa tttatgtgtt gttaccgcac ttccaacaac agatgaaaaa
    23641 tctgcagctg gtatttcttt aaaatcaaaa taaactggct gtaaattatt tatttcgcaa
    23701 ctttgcggag ctgtaatatc gccccgaata taaacatcag ccatcggctg tgagtaatcc
    23761 accggggtgt cacggctaat tgttccatac aatgtcacta tctttgtttc aggaataatc
    23821 aattttcctg caaatgtctt cttgatataa aaatatacta tcgctttagc cccactggat
    23881 atacttaaag gttcaatgcg gttgcaattg tactttgtat taccagacgg gttgttagca
    23941 tgatattcaa aaggtgcatt cacataccca accccaagaa tatctatagc aatcccaaca
    24001 tcaagattcg gattaagatc ataataatta atattagagt gaaccccagc ataagttaat
    24061 gcctggttta tttttgacgt ataatagata taggggaatt ctcctacgtt accagtatca
    24121 caatgacagc gtccaggata tgaggatcca ttagttacag tggtgttaaa tgtcgttcca
    24181 accttatttt cgttcgcggt aataagatat cccgagaaat caagcgtact gtgaaatacg
    24241 ccattatcag gggtacagat acctttagga gctgcaagtg ctacactact taacgagagc
    24301 ataaacacag caaagcacca ctgtttgata tacttgttca taaaaatcct tttaatgaca
    24361 tgtcacaccc tgttcaatta atccgctctc actttctggt gtcgaaagtg aataattgaa
    24421 gatgcatttt gttgaggcgt tttctcccca ggataacgta acctgtcctt tttctggtag
    24481 accagaaaga taaagttcgc cacggtcacc gacaattcct gaatctgcca aacttgcatc
    24541 ttgtatcgca gcaatggctc caaaaggtac cggtttacca tttgcattta acactctgaa
    24601 gagaacgcga taacccacat gagtcacaaa ctccgcttta accactgcac cacgcgtggg
    24661 aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat agttatcgct
    24721 gattgctacc cgattttcat cataagggtt tagctgtgtc ttgaccgtat agccgcgcca
    24781 gtccgtcgca gcaccgcgca tattatctat ttctaacccg gcggccccag gtgctttaac
    24841 aagagcaata gtttcaccta actcctgact taacgttacc ccttcgctgt gtgccagaat
    24901 tccccctctg ataccataat tcagcctctg ggaattttgg ctgtagttat agctgccgtt
    24961 caccgtgcca taagagccat cataggtcag ggacatatta ccgccaacct catcatcatg
    25021 actgttatag gactgcatta aattccatga aaggttattt cgttccagcg ccgtaccgct
    25081 gagtccgagt tgatgtgagc taccggaatt atcgttttgc gaaacagaat aggtggcgta
    25141 actattaccc caccagcccg ataaaggaac agataaagag agcatgtaga tctgctcact
    25201 ggtgttatca ctggcatggt tgttccccgt cccctgtctg gcagtattgg aataactcca
    25261 ggagacgttc cacgagagac gcttccagct actgctgtag ccaaattgca ttaatcgttc
    25321 gacatcatcg gtatgccagt attgttgccg gcttaatgtc aggtataaat tgccataatc
    25381 tctccccaac gactgcgtta gcgttccctc cacgcgacta cgtcggttac ctgtttccca
    25441 aaagtcttca gggctgttgc gccgcgatgc ccactcattg agggtatagt agccctcggt
    25501 ggagtaacga tagccaacca ggcgcaagct agtgtctgtg tcgtcaaatc gttttgaata
    25561 gagaaagcga tatgactgac ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg
    25621 tgtcacatcg aaagaaattg cgcccaaagc gccaagatct ttaccgacac ccagagctgc
    25681 agaaccataa ttttcagcag cctgcatacc tccatataaa gtgatcccgt gggaaaaacc
    25741 atgaagtatt tgcgcctgaa ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc
    25801 cacgctgaca tcaacatctg tctgaccttc acgtttaaga atcgccaatg aagcataagg
    25861 ttgggtgaat cgttgttcgc taccgtcttc ttctttgatg gttacgtcca aatcgccacc
    25921 tgtactggcg gtgttgagat cggtgatctc aaaagcacct tgtggaacgg cagactgata
    25981 aatcacgtag ccattctgcc gaatgatgac ggtggcgttg gactttgcaa tcccacgaac
    26041 cacaggagca aagccattct ggctggcggg tagcatatcg ttatcagtat acaaacgcgc
    26101 gccgcgaatt tgcgtactgt cgaaaatatc gctcgccgtc cacgtatcac caatcataat
    26161 ctggcttctt aacgcggcaa tatcgcgctg tatccaggta ctaatggagt tgtattccgc
    26221 acggccatcg ctggtgttta aagtactgta attacgtagc cgccacggtc caatattcag
    26281 tccattgcgc agattgagat aactggtatc tgtctcttgt gaaccataat ctgaacgtag
    26341 tgtatgtgaa ccagaaaaat cgtagttgat ggtgagcgcg ttaatcccct catcccaacg
    26401 cgtggggctg atgtaatcat aagccatcgc gtccatgtag atttggggaa tggatatatt
    26461 cagctgcaat ttatcactgt caaactgcca ggatgagcct tcaatacgtg gagcaggatc
    26521 gaggcaaacc gtctcatcca catcattgaa cgatttgagc gcatcaactt ttatgccaaa
    26581 ggaggacagt gtttttttaa tggcaataaa gcaagcgtgc agatcagcat ctggattatc
    26641 ctgaacaaat gtcacatcct gacgttcaaa cattttaccg ttgataaaaa tatcaacgct
    26701 ataaacacct gggggcatca ggtttgcccg attaaactgg cgaatatcga ctccggattg
    26761 atggtcgatt tccagaagcg ccggattgaa tacactgacc tcggccatca ggttttgcga
    26821 gaaaatcaga cacgtccctg ccgtgaggcc aaccactatt ctggtcgtca tcataatacg
    26881 cctatcctta tcccttgctt acagcgtcat ttttatcggg tcactgactg cgccgtaatc
    26941 attaataaat cgccaggtca tttggcctcc cgcagaatta gcaggtaaag gtaacgtgag
    27001 cgtttcaaaa ggacggatca tattgagggg cacatcatgg ccatcaacga tgacatccat
    27061 cacactgata tgaattgggt tgggattctc tgctatcaga tttcgccctt cccggcgcca
    27121 tttcactttc ggcgcagcat cttcgggaat aaggcggata tttgctggtc gccagaacag
    27181 cttgatacgc gtttttactg ctatttccag cgagttcgtc gctttgttgc tcgctgatgg
    27241 cggaatattt ttaatattca gccagaagac tgactcgcga tcatcaggca atgacacacc
    27301 tttggtgtga ataatgcgta atattttttc ttgcgaaggt tctaatcgtg atactggcgg
    27361 aataacggta aacggcgctt tgcttttatc ctctttatta aatggatcta cccaagcctg
    27421 gatgagataa ggtatttttt cttctttgct aaatacagaa atagatttat cagcattatt
    27481 acttaagtaa accacccgcg taccaccaat gacaacgcct gcataagtta atgctggttg
    27541 gcctaaaaaa acgctgagta taatcgcggt aaataactta catgaaactg ttgttttaaa
    27601 gagcctgatt aaacaacttt ttctggacat aacgactccc tggccaaagc caactgaata
    27661 aaaaggccct tgataaatta tttcttatat tcgacagtaa attgtgaatc agcgttcgcc
    27721 gtaccagccg ttacggtcgc attggttgcc acatagcgtg caataaactt catttcagcc
    27781 ttgccttcag catcgatagc ggtataagca gagtcgttat aaagtttaac ctgcgaatta
    27841 tcggactggt taaaaatacg aataccaacg ccggttgccg caatcgcttg tcctgtaccg
    27901 gtaaaatcac cagggttgcc tgctgtaagc ggcgtaccca ctttcaggtc gccgtcgccg
    27961 ccaggcgctt ctgtaccatc aaaacgaacg gctgcttgag tgtacgttgc cgggcaattt
    28021 tcaagcttaa tactgaactt ctgtggcgac gccagactac ctacacctga aaatgcattt
    28081 ttactgactt tacccagtgg gacaatttga tcggctgtat cggaggtaac ttcacaagta
    28141 gtgtcgacaa tttcaccgac gaagtgaaca acaccatctt ctgccagtgc atgcccggcc
    28201 agcattagag atgccagagt gaatgcaccg cctataatat tacgcttcat aaaaa
//
LOCUS       DAZUFB010000057        26967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZUFB010000057 DAZUFB010000000
VERSION     DAZUFB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.057"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..1676
                     /gene="rtcR"
                     /locus_tag="ACHY3M_002775"
     CDS             78..1676
                     /gene="rtcR"
                     /locus_tag="ACHY3M_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFS4317663.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1658..2416)
                     /locus_tag="ACHY3M_002776"
     CDS             complement(1658..2416)
                     /locus_tag="ACHY3M_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFS4317664.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2433..3263)
                     /gene="glpG"
                     /locus_tag="ACHY3M_002777"
     CDS             complement(2433..3263)
                     /gene="glpG"
                     /locus_tag="ACHY3M_002777"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFS4317665.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3308..3634)
                     /gene="glpE"
                     /locus_tag="ACHY3M_002778"
     CDS             complement(3308..3634)
                     /gene="glpE"
                     /locus_tag="ACHY3M_002778"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFS4317666.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3824..5329
                     /gene="glpD"
                     /locus_tag="ACHY3M_002779"
     CDS             3824..5329
                     /gene="glpD"
                     /locus_tag="ACHY3M_002779"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFS4317667.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5381..5986)
                     /locus_tag="ACHY3M_002780"
     CDS             complement(5381..5986)
                     /locus_tag="ACHY3M_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317668.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5989..6756)
                     /locus_tag="ACHY3M_002781"
     CDS             complement(5989..6756)
                     /locus_tag="ACHY3M_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317669.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6759..7469)
                     /locus_tag="ACHY3M_002782"
     CDS             complement(6759..7469)
                     /locus_tag="ACHY3M_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317670.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7485..8993)
                     /locus_tag="ACHY3M_002783"
     CDS             complement(7485..8993)
                     /locus_tag="ACHY3M_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4317671.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRLISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9119..11566)
                     /gene="glgP"
                     /locus_tag="ACHY3M_002784"
     CDS             complement(9119..11566)
                     /gene="glgP"
                     /locus_tag="ACHY3M_002784"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFS4317672.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11585..13018)
                     /gene="glgA"
                     /locus_tag="ACHY3M_002785"
     CDS             complement(11585..13018)
                     /gene="glgA"
                     /locus_tag="ACHY3M_002785"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFS4317673.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13018..14313)
                     /gene="glgC"
                     /locus_tag="ACHY3M_002786"
     CDS             complement(13018..14313)
                     /gene="glgC"
                     /locus_tag="ACHY3M_002786"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFS4317674.1"
                     /translation="MVSLEKSDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14331..16304)
                     /gene="glgX"
                     /locus_tag="ACHY3M_002787"
     CDS             complement(14331..16304)
                     /gene="glgX"
                     /locus_tag="ACHY3M_002787"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFS4317675.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYVWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16301..18487)
                     /gene="glgB"
                     /locus_tag="ACHY3M_002788"
     CDS             complement(16301..18487)
                     /gene="glgB"
                     /locus_tag="ACHY3M_002788"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFS4317676.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18760..19863)
                     /gene="asd"
                     /locus_tag="ACHY3M_002789"
     CDS             complement(18760..19863)
                     /gene="asd"
                     /locus_tag="ACHY3M_002789"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4317677.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20055..20648
                     /gene="yhgN"
                     /locus_tag="ACHY3M_002790"
     CDS             20055..20648
                     /gene="yhgN"
                     /locus_tag="ACHY3M_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFS4317678.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20705..22045)
                     /gene="gntU"
                     /locus_tag="ACHY3M_002791"
     CDS             complement(20705..22045)
                     /gene="gntU"
                     /locus_tag="ACHY3M_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS4317679.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPVLGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22049..22576)
                     /gene="gntK"
                     /locus_tag="ACHY3M_002792"
     CDS             complement(22049..22576)
                     /gene="gntK"
                     /locus_tag="ACHY3M_002792"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4317680.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22715..23710)
                     /gene="gntR"
                     /locus_tag="ACHY3M_002793"
     CDS             complement(22715..23710)
                     /gene="gntR"
                     /locus_tag="ACHY3M_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFS4317681.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23934..24629)
                     /gene="yhhW"
                     /locus_tag="ACHY3M_002794"
     CDS             complement(23934..24629)
                     /gene="yhhW"
                     /locus_tag="ACHY3M_002794"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFS4317682.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24752..25789)
                     /gene="yhhX"
                     /locus_tag="ACHY3M_002795"
     CDS             complement(24752..25789)
                     /gene="yhhX"
                     /locus_tag="ACHY3M_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4317683.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26121..26609
                     /gene="yhhY"
                     /locus_tag="ACHY3M_002796"
     CDS             26121..26609
                     /gene="yhhY"
                     /locus_tag="ACHY3M_002796"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS4317684.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            26846..>26967
                     /locus_tag="ACHY3M_002797"
     CDS             26846..>26967
                     /locus_tag="ACHY3M_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFS4317685.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFT"
BASE COUNT         6754 a         7192 c         6710 g         6311 t
ORIGIN      
        1 ttacgtccgt aaacggagat tttccacaaa accaattcac cgataatgaa atatcgtctt
       61 ttataaggat atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg
      121 attatgccgg acgcggcagt cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc
      181 agcaagaatc gttagtcatc gatcggctgg aattgttgca cgacacccgc tcgcgctcgc
      241 tattcgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtcgtgggcg
      301 ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt
      361 tcgcccgtgg ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg
      421 gcacccacgt cgcgcagatt tgctggtttc tgctggcgga agcgcgttac ctgcccgccc
      481 ggctgataca atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga
      541 cgattatcga tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac
      601 gccagcaaac gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc
      661 gcatgattga gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg
      721 gtccaaccgg cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc
      781 ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg
      841 ccatgtcgac gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg
      901 aaggtttatt acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg
      961 gcgcagacga acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg
     1021 gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc
     1081 gccagctggt tgccgaaggc aaatttcgcg aagatctgta cacgcggatc aatctctgga
     1141 ccttcaccct gccgggtcta cgccagcgcc aggaagatat tgaaccgaac ctggattatg
     1201 aagtggagcg ccacgcctca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc
     1261 gcgcctggtt ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac
     1321 tttctgccag cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg
     1381 tggttgaaga tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc
     1441 ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg
     1501 aacacgttat cgctatctgc cgccaggcaa agtcgctttc cgcagccgga cgccagcttt
     1561 ttgacgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc
     1621 tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat
     1681 ggtggtccgt cagcacctgc atcacgctta ctggcggcgg ggcgtcggtg tagacggcat
     1741 ctaccatgct gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga
     1801 caaccagcat aacgtggcgc gagttctcaa taatggcgcg tttggtgcga acttcgtggt
     1861 aatcgaactc cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat
     1921 caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc
     1981 tgcgtaattc gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag
     2041 caacattgag attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca
     2101 ccgcttccgg cgtggtgccg atatcgataa acagtgtcga gccattgggg atttgctccg
     2161 ccactttgcg ggcaatcctc tctttttctt cggtctgggt ggctttacga tcgtgccacg
     2221 gcgtgttaac cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt
     2281 gctccgccag ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta
     2341 ccagctcttc agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac
     2401 gttgtgtttg tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa
     2461 tcaacaaaag ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc
     2521 gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac
     2581 ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc cacgtagcca gacgtagccc
     2641 atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc
     2701 acatagccgc ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg
     2761 cgtttttcca ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc
     2821 agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga
     2881 tcgaatggcc aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata
     2941 aacaccacca cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag
     3001 gcagcaaaga aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag
     3061 ctggccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc
     3121 acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc
     3181 gtgaggataa caccctgcgt cgccatataa tcaacaaacg cctgcgccac gcgggggtta
     3241 gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta
     3301 taaagcgtta cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac
     3361 cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt
     3421 tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca
     3481 taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc cccatagcga
     3541 aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg
     3601 cgtcggcaac gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt
     3661 gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat
     3721 atcactctaa aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga
     3781 taacgaacat ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct
     3841 gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg
     3901 tttatccgtg ctgatgctgg aggcgcagga tctcgcttgt gcgacctctt ccgccagttc
     3961 aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga
     4021 ggcgctggct gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg
     4081 ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt
     4141 tatgtacgat catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg
     4201 cgcaaactca gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt
     4261 agacgacgcc cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt
     4321 gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc
     4381 ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac
     4441 cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat
     4501 tcgcctgatc aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta
     4561 cattctgcaa aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc
     4621 catcatcggc accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga
     4681 gagtgaaatc aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg
     4741 tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc
     4801 gccgcaggct attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc
     4861 gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct
     4921 ggaaaaactg acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct
     4981 gccgggtggt gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta
     5041 tccattcctg actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga
     5101 gctgctgctc ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt
     5161 ctacgaagcg gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc
     5221 cctttggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag
     5281 tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc
     5341 ggttgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
     5401 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
     5461 tctggtttct attttttctg caccaatggc aataatatcc ccatgcaaaa ccaagctgtg
     5521 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
     5581 gaggccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
     5641 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
     5701 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
     5761 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct
     5821 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
     5881 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
     5941 caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg
     6001 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc
     6061 ccagacctga tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg
     6121 cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat
     6181 ggcgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
     6241 acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
     6301 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
     6361 atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc
     6421 atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg
     6481 aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
     6541 ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata
     6601 cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata
     6661 ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg
     6721 ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg
     6781 cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta
     6841 cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg
     6901 caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat
     6961 ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag
     7021 acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca
     7081 tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat
     7141 tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa
     7201 agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct ggaggttaag
     7261 ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac
     7321 ttttcccctt catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat
     7381 gccgatttgt agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg
     7441 cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta
     7501 tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac
     7561 tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa ggcatcgacg
     7621 atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct
     7681 agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct
     7741 attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt
     7801 gccacttctg tttcctgcgg cgagatcagg cggacatttt tcatgacctg ggattgcatc
     7861 accgtctggc taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg
     7921 ccaacagtac gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc
     7981 agggaattaa ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc
     8041 gtgtacagca aagtaacgcc cgattcctca gcgcgttttt tctgcccacc ggaaggttgc
     8101 gccacaaaaa taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga
     8161 gagctctcca gatattccct ggtgtgaaaa tcttccggta taacgcttaa agcataaaat
     8221 gcagaagcat aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta
     8281 aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc
     8341 caggcccagg tatcaatatt ttcgcgcaca ccgacgccgg gattgagctg tttatataaa
     8401 taaccctgat aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg
     8461 caacgtagcc cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa
     8521 aaactgatat gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact
     8581 gagactaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc
     8641 tggtgaaatt gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa
     8701 ccgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta
     8761 tcgcctgccc atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa
     8821 atgttgagca gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg
     8881 gcaagtccga cacccagcat agcaacaacc gccgccaaaa attgcgtcag tatggggatg
     8941 ccgaaaaaag tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc
     9001 attctttttg aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt
     9061 ccgaaatgat ggcggaaaaa aacgggaccc tttggccccg ttctatttat tggtgaactt
     9121 acaatctcac cggatcgata tgccagatat gatcggcgta ctctttgata gtacggtcag
     9181 aagagaagta gcccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa
     9241 gctcgtagag ttcgtcgact ttatcctgac aatcgacata gctgcgataa tccgccagaa
     9301 cctgatagtg atcgccgaag ttgatcagcg aatcaaccag atcgcgatag cgacccggat
     9361 cttccggact gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt
     9421 tctcgtagta ttcacgcggt ttgtagccct gacgacgcag ttcttccact tcttccgcag
     9481 tgttaccaaa gataaagata ttgtcagcac cgacatgatc cagcatctcg acgttcgcac
     9541 cgtccagcgt accgatagtc agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg
     9601 aagcttccgt ccctgccagc gaaatctgtt cagacagatc tgccgccgga atgatcaact
     9661 gcgccaggct aacgctgtag ttcgggatga acacgacttt cagcttatcg ccaatctgcg
     9721 gatcgttgtt gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt
     9781 aataggccga agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg
     9841 ggtcggcctt gatgcggtta tagcgggtaa tcacatgcaa cacattcatc aattgacgtt
     9901 tgtattcgtg aatacgtttg atttgtacat cgaacaacgc ctttggattc accaccacat
     9961 tcagctgctg ggcgatatac tctgccagac gctttttgtt ctccagcttc gcctgatgca
    10021 cagcgtgatt aaccattggg aaatcacagt gttgttgcag ctcattaagc aggctaaggt
    10081 cggtgcgcca gttacggccc aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta
    10141 ccgctagcca gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga
    10201 ttttcgcaaa gtcggcaaac aacgattgca ccatcagatt agagtgcagt tccgatacac
    10261 cgttaacttt gtggctcaca acaaccgcca gccaggccat acgcacacga cgaccgttgg
    10321 attcatcaat gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca
    10381 aggttttcag gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt
    10441 tacccagcat atcaaccggc caggtttcca gcgcctcgct catcagcgtg tggttagtgt
    10501 aggagaagac ctgacaacac acctcaaacg cgtcgtccca gctaaattgg tgctcatcga
    10561 tcagcagacg catcatctca ggaatcgaca gtaccggatg ggtatcattg agatgaatcg
    10621 cgattttatc cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt
    10681 cctgaatggt cgaggaaacc aggaagtatt cctgacgcag gcgcagctca cgcccggagt
    10741 aggtggagtc atccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca
    10801 ctgccgcgaa gtagtcaccc tggttgaatt taccgaggtt aatttcgcta ctggcttgcg
    10861 cactccacaa acgcaacgtg ttggtcgcgt cggtgtcgta accagggatt atctgatcgt
    10921 aagcgactcc cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa
    10981 tgcgaccacc aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac
    11041 cgtattccag ccagtagtct ggcgactctt tctggctacc gttaacgatg ttctgcttga
    11101 acataccgta gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag
    11161 aatcaaggaa gcaagccgcc agacgtccca ggccaccgtt accgaggcct gggtcatttt
    11221 cttcatcaat cagctcttcg agatttaacc ccatcgcttc cagtgcgccc tgtacatctt
    11281 cgtaaattcc tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg
    11341 acaggtagta aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc
    11401 gctccacgag acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg
    11461 cgacgaccgg gtcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag
    11521 cttctacgct aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg
    11581 aaaactattt caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa
    11641 aatccattgc catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta
    11701 caaaagcacg tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc
    11761 cactggcgac gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc
    11821 caccggtgcg ccgcactaac ggcagtgtac cgtacttcaa tccataaagt tgcgttaagc
    11881 cgcacggttc aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg
    11941 aaaatgcttc gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg
    12001 caaggaaacc ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct
    12061 gctccagaag gcccggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac
    12121 ggctcaccac tgcaaaaagc ggcactttat catcaacctt aagccccatt gcgatttgta
    12181 actggcgctt attttccgct ttatcttcca acgtatcgcg ggtgtagcgc gaggccaaca
    12241 gtaagtccgt ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac
    12301 gcccttcacg gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg
    12361 tgatctcgcg agcgtaggtt ggactaaccg ccgtaatgtg atcggcatag tacagaccgg
    12421 ccttcaggaa agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg
    12481 gcaattggat gtcattcata tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca
    12541 cagtaaacac cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg
    12601 catgccagtc gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca
    12661 tttctgcccc aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata
    12721 agttggtatc gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt
    12781 atatgccaac cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac
    12841 gacgggatac tacctgcgca tcggtaacgc cacggcgaat atcgggaaat gcaggcaaca
    12901 gtacgcgagc gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag
    12961 ccagaccgcc ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta
    13021 tcgctcctgt ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc
    13081 ttctgaacga tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc
    13141 cggaataaca caagcacgat cgatgacgca gcggcgcaga cggcacgagc gacctaccca
    13201 tacttccggt aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga
    13261 gaacagaacg gactgcacca ccaccgaacc ggagatcaca cagccgccgg ataccagtga
    13321 gttaagggtc atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga
    13381 ttcattgtag gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga
    13441 ggccagatcg aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg
    13501 ctcggcatcc gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc
    13561 ggcttcggtg atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcacg
    13621 atcgtcttct tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact
    13681 cgccagagat ttgctcggat cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa
    13741 ttcgataatt ttatcgttct catcaaccgc cataacgcca aatgcggagg cttcttcaat
    13801 cggtactggc atacaagcaa cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat
    13861 acgcgagtag tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt
    13921 ataacggcgg ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt
    13981 ttcccctttc attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt
    14041 gaagaatgac cagccgcgct gaatgtgctg caccagagtg tgggactggt actgggtgat
    14101 cacgcccata cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg
    14161 gaacttaccg ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg
    14221 ggtaccacgt cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgcgccaa
    14281 cattaagtga tcgctcttct ctaaactaac catgactaac tcctttttta tcatctctgg
    14341 aacacacaca atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct
    14401 ccagcgaatg ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca
    14461 agcgtggcgt taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc
    14521 ccgttttgcc actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg
    14581 ccgtcgcctt cttcccacca gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg
    14641 attaacgcgg cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt
    14701 aattggttat cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca
    14761 ccggccagta acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg
    14821 ctgtcgcgcc gccgttcaac caggtcaaga gaaccgccta acccttcttt accatgattg
    14881 ttactgtaat tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta
    14941 tggttgaagc aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc
    15001 gcggcactcg gcagacgacc attacgttta aaaacatcgc tggaggcagc aaaacgcccg
    15061 gcaaacgccc ccagaggcaa atcataatgt agccagaaac gacgggcagc atcgcgaaaa
    15121 tgatcgttcc actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg
    15181 atatcccacg gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg
    15241 gtaaacaacg gcgcatcctg acggaactct ggcgtacggc ccatgactgc cgccagatca
    15301 aagcggaaac catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa
    15361 tccaccaccg ccggatgact caaattgagc gtgttgccgc aaccggtcca gttgtgataa
    15421 tcgccgtctt ctcttatcca ataatagcta cggttatcga tcccacgcag cgagaataac
    15481 gggccgtcga ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata
    15541 cccgctttat gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag
    15601 caggcatacg ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttactt
    15661 agccccatgc gttgcagacg tggttcactg gcaaactgcg ccactggcag cagttccagc
    15721 gcggtaatgc ccaattgttt caaatagttg atcatcaccg gatgcccgag ggctttataa
    15781 gtgccacgga tctcgaccgg gatctccggg tgcaagtacg ttaatccttt gacatgggct
    15841 tcataaatga tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagacatag
    15901 tgatcaacca ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca
    15961 ttatgaccgg cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa
    16021 ggatcaatca acaacttcgc cgggttaaag cgatgcccct cggcgggttg ccaggggcca
    16081 tgaacgcgat aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg
    16141 tcgccactgt gccctggcaa gtcatagcga tgttcctggc cattggcgtc aaagacacac
    16201 agttctaccc gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa
    16261 tgcgcgccga ggggagcggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca
    16321 gccagatagt ggccagcggt ggtagcgtca ggcttagtga atgctgacga ccgtggctgg
    16381 caatctcatc gctgtgtacc gtgccgccat tgcctgcatt actgccgtga tagtgcatgg
    16441 aatcggtatt gaggatttca cgccatttgc ccggctggtt tatgccgaag cgataatcat
    16501 gacgcggtac cggcgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac
    16561 gcacaaagat cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg
    16621 ggtcaaaatc cagttcatgc attgctttat ggtggcggta ggtgaggttc agatcgcgca
    16681 ccagacgctg gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc
    16741 tggcgtcatg gttccactcg cggccctggg caaattcgtt acccatgaac agtagtttct
    16801 tgcccgggaa tgcccacatc cagccatagt aggcgcgcag gttcgcgaat ttctgccatg
    16861 cgtcgcccgg catgcggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca
    16921 acggcaggac gaagttttca gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat
    16981 gatactgacg ataaaccggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga
    17041 ggttccactt gtaccagaag cccagaccgc ccatatcctg cggacgagaa acgccaggga
    17101 aatcggtaga ctcctcagcc attgtcaccg caccggaaac ctgctcacca agaatacggt
    17161 tggtattacg caagaattca atcgcttcaa gattctcgcg cccgccaaat tcgttcggga
    17221 tccactcccc ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc
    17281 gcagcgcatc aataccaaaa cgttcaatcc agtaaagcgc gttaccgacg aggaagttac
    17341 tgacttcacg gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcac
    17401 gcggatcgct gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcag
    17461 tcgggaagtg gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat
    17521 caatgaaata acggaagtcg tcgcgagtac caaaacggcg ggttggtgca tacaggccgg
    17581 ttggctgata accccaactg ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt
    17641 gggtaaagcc catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca
    17701 accagaaatt gttgtcggtg tggcgacgcc aggaacccag gtgaacttca taaatagaga
    17761 ttggcgcatc aaactgattc gcttttttgc gctcttcagt ctgtacaacc ttttccggca
    17821 gcccgcaaat aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg
    17881 acttcagacg caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat
    17941 gcgccccagg gataaacagt tcccagatgc cgctctcttt acgcaggcgc atcgggtgac
    18001 ggcgaccgtc ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc
    18061 agacagagaa acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt
    18121 catacgggcg caggtgagta ccttcagata atagccaggc atccatttcc tggattagcg
    18181 gaccaaaacg gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact
    18241 gatagcggaa aaaattctta cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga
    18301 gacactccag ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag
    18361 cgtcgggtaa aagggcacgg acttccagtc ccgcggtggt tttatgcatt cccagtacgg
    18421 aaaaaggatc cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat
    18481 cggacatgct tgtcttcctg ttttattgtg tcaccccatc caattggatt tttgacttct
    18541 ggttgtgaca tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc
    18601 ctcacttcag gtaaggctgt gaatactcat gtattcagcc acccttaaag aatagccaat
    18661 gctctattta actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta
    18721 acaggggcgg catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat
    18781 ccgacgcagc ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag
    18841 gaactctggt cccatattca gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt
    18901 aacggcagct ggggttagct cacgcatagt gatttcccga tcgttcggaa cgactttcgc
    18961 ccacggattg tgcgcagcca gcagttcttc cacggtcgga atagacacat cttttttcaa
    19021 tttaatagtg aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc
    19081 taccggaatt acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc
    19141 ttcgcggctc tgaccgttat cgagctgttt gtcgatccac ggaatcaggc tacccgccag
    19201 cggcacgcca aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg
    19261 ttcgatatcg agaatagcag aggacggggt cgcgagttca tctgccacat ggccatacag
    19321 atggcccatc tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg
    19381 gtaggttgca acggacaccc aatcaacaag atcattggcg aataaaccac ccaacgacat
    19441 caacatcagg cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc
    19501 gtcggtaatg acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg
    19561 cagagacgat gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata
    19621 gatttcgttg gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc
    19681 ctccagatca aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg
    19741 gccaagctga gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac
    19801 catgcgttgc atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt
    19861 cataagcgtt ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca
    19921 catctttcac catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa
    19981 gccatcagta atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc
    20041 agggaatttg agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg
    20101 atccgctcgg aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac
    20161 ggcgggcaat catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt
    20221 ttgcgggtga gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg
    20281 gcggcatcat tctgtttcta atcgccatta agatgatttt ccccagcgct tcaggaaata
    20341 gcagcgggct tccggcaggt gaagagccat ttatcgtgcc gttggctatt ccgttagtcg
    20401 ccgggccgac tattctcgcc acgctgatgt tgctgtctca ccagtaccca aatcagatgg
    20461 gacatctggt gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt
    20521 cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg
    20581 gattgattct ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga
    20641 aggggtaacg aggagagttt ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac
    20701 aaacttaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga
    20761 tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac
    20821 cgaacaacca gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccagcgatac
    20881 aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca
    20941 ccagtcctac cgccgttaaa caggctacgg tggcagaacc ctgaatgatg cgcactgcag
    21001 cggccagcac gaagcaggtg atggctatcg gcaggcccat gccagttaac gcttcgccca
    21061 gtaccggacc tacgccagag tcaaccagca cctgtttgaa cacgccacct gcaccaatca
    21121 ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt
    21181 ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg
    21241 cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct tctggcacaa
    21301 aacgcgcggc aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc
    21361 tgaatccgaa agatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa
    21421 tatgcaactc cacgtagcgg ctgatgaaat taccccacag cggcccggca ataatcattc
    21481 ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg
    21541 atgccagcag cattggcgct ggccctggca ccaggaacgc cgccgctgcc gccacgcctg
    21601 cgaataatgg gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag
    21661 caacgctaat cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc
    21721 ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg
    21781 caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga
    21841 caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt
    21901 tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga
    21961 aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg
    22021 ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc
    22081 ttgccacaac accttccagc ggttgatcga tatccaccac cagtacatca gtttcgtccg
    22141 cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc
    22201 ctttgcgcgc tttcaggcgg ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag
    22261 agagattcgg attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga
    22321 cgatcagtga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca
    22381 accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc
    22441 gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac
    22501 tggcgaccgc agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat
    22561 ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac
    22621 tgttatcggt aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa
    22681 gtgtataagt gtgagctact tcaaatttgt aggcttaaat agatccgccc ggtgacaagg
    22741 tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc
    22801 gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgct aaccgcggct
    22861 ccatcacctg gccaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaacct
    22921 tcaatcccag acgctgacat tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga
    22981 acacgccatc cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag
    23041 agtaagaaga agattgctca accatcacgc tatacggcac cagacctgca tccagcatcg
    23101 cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg
    23161 caatgtggca atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt
    23221 tatcaaaacc gacggcgata tcgaggcacg gcgactggct gtccatgagt tccaccaccg
    23281 ggatccccgc cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga
    23341 tcagtccgtc gatattccat gagagcatgg attcgagacg ttcttgctcc atttccggtt
    23401 tataaccgta gtgcgccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc
    23461 cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc
    23521 ggctggtggc gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa
    23581 gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac
    23641 ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt
    23701 tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca
    23761 tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa
    23821 ttgcatcgca ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc
    23881 ggagcgagcg aggaagcctg caaaaaggca ggcttcaaaa atgccgtgga gctttaaacc
    23941 ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc
    24001 tcatcccaga ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg
    24061 cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca
    24121 tctttcagca acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct
    24181 cgcgcatccg gcgagagcac cagctgtttg ccctgtacgg catcgaagcg acgctgttca
    24241 taacgcggcg taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc
    24301 tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg
    24361 aactcacccg ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct
    24421 tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct
    24481 gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg
    24541 taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca
    24601 cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg
    24661 cttagtgtgg acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa
    24721 aaaaattgaa ctattcgagg ggccatcaaa cttaattcgc gagagtcact gtggagggag
    24781 aggcttgctc aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga
    24841 cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat
    24901 cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca
    24961 gcacaccgac cgaattatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca
    25021 ggctggtttc ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga
    25081 taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc
    25141 cataaaacag ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcgga
    25201 tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc attgtatgca
    25261 caccaaggcc ataaaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc
    25321 ggtaatagtc aaaatggctt tccacttcaa caatctctcc caacttgcca ctttcaatcg
    25381 cttttttcgc tgtcaggaag caggagtcaa agcgacgatt ctgatacggc gtgacggtca
    25441 gccctttgct tttcgccaac gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg
    25501 gtttttcgac cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc
    25561 tgtccgcgtg ggtgcagaca acaaccagct taacatcggg atcgtttagt acttcgtcga
    25621 gatcgctggt gaaatggata tgggaataaa tgggagcctg ttcttccggc ttcgcatggc
    25681 gacgaaaaat atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt
    25741 aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct
    25801 ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca
    25861 taacgaacac aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc
    25921 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
    25981 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
    26041 tttttgcaaa aaatgcattt gactcgcatt tgaaagtcaa tgatgttgaa agggacattt
    26101 accccaaaga ggacaaagga atgagtgaga tagtaatacg ccacgcagaa acacgggatt
    26161 acgaggccat caggcagatt cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc
    26221 ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc atcaagcaac
    26281 tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg caacagcgcc
    26341 cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctctcgttgg aagaaccgcg
    26401 gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag
    26461 atcgcattga actaaccgtg tttgtcgata acgccccggc aattaaggtc tataaaaaat
    26521 acggctttga aattgaaggg actggtaaga agtacgcatt gcgtaatggt gaatatgtcg
    26581 atgcatatta tatggcgcgg gtgaagtaag atagtgccct ttttctgaga tggaaaaagg
    26641 gtgtcattca aaatcgacat accttccttt aaggtattta tttgcccaat acatatattg
    26701 agatttaact cataattact tcctaaagtg taatatttta ttttttaata tatacgccta
    26761 caatttcctg gagtaaataa ataacaatta acaagcataa tattgccatt gataaaatag
    26821 catgccataa aaggactttt cagggatgag taatattgtt tacctgacag taacgggaga
    26881 acaacaagga agcatctccg caggttgtgg gacttctgag tctacaggta atcgttggca
    26941 gagcgggcat gaggatgaaa tatttac
//
LOCUS       DAZUFB010000058        26810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZUFB010000058 DAZUFB010000000
VERSION     DAZUFB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.058"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..152
                     /locus_tag="ACHY3M_002798"
     ncRNA           28..152
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_002798"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            401..1444
                     /gene="nadA"
                     /locus_tag="ACHY3M_002799"
     CDS             401..1444
                     /gene="nadA"
                     /locus_tag="ACHY3M_002799"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFS4317686.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1482..2201
                     /gene="pnuC"
                     /locus_tag="ACHY3M_002800"
     CDS             1482..2201
                     /gene="pnuC"
                     /locus_tag="ACHY3M_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4317687.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2198..3139)
                     /gene="zitB"
                     /locus_tag="ACHY3M_002801"
     CDS             complement(2198..3139)
                     /gene="zitB"
                     /locus_tag="ACHY3M_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFS4317688.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNKGVSGHSHHHH"
     gene            complement(3253..3633)
                     /locus_tag="ACHY3M_002802"
     CDS             complement(3253..3633)
                     /locus_tag="ACHY3M_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFS4317689.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3949..5001
                     /gene="aroG"
                     /locus_tag="ACHY3M_002803"
     CDS             3949..5001
                     /gene="aroG"
                     /locus_tag="ACHY3M_002803"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFS4317690.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5160..5912)
                     /gene="gpmA"
                     /locus_tag="ACHY3M_002804"
     CDS             complement(5160..5912)
                     /gene="gpmA"
                     /locus_tag="ACHY3M_002804"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4317691.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6115..7155)
                     /gene="galM"
                     /locus_tag="ACHY3M_002805"
     CDS             complement(6115..7155)
                     /gene="galM"
                     /locus_tag="ACHY3M_002805"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFS4317692.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7149..8297)
                     /gene="galK"
                     /locus_tag="ACHY3M_002806"
     CDS             complement(7149..8297)
                     /gene="galK"
                     /locus_tag="ACHY3M_002806"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4317693.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8301..9347)
                     /gene="galT"
                     /locus_tag="ACHY3M_002807"
     CDS             complement(8301..9347)
                     /gene="galT"
                     /locus_tag="ACHY3M_002807"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFS4317694.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9357..10373)
                     /gene="galE"
                     /locus_tag="ACHY3M_002808"
     CDS             complement(9357..10373)
                     /gene="galE"
                     /locus_tag="ACHY3M_002808"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4317695.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10634..12106)
                     /gene="modF"
                     /locus_tag="ACHY3M_002809"
     CDS             complement(10634..12106)
                     /gene="modF"
                     /locus_tag="ACHY3M_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFS4317696.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(12174..12962)
                     /gene="modE"
                     /locus_tag="ACHY3M_002810"
     CDS             complement(12174..12962)
                     /gene="modE"
                     /locus_tag="ACHY3M_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFS4317697.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            13091..13240
                     /gene="acrZ"
                     /locus_tag="ACHY3M_002811"
     CDS             13091..13240
                     /gene="acrZ"
                     /locus_tag="ACHY3M_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFS4317698.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13406..14179
                     /gene="modA"
                     /locus_tag="ACHY3M_002812"
     CDS             13406..14179
                     /gene="modA"
                     /locus_tag="ACHY3M_002812"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4317699.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGSQAAEIFKRYGFTTK"
     gene            14179..14868
                     /gene="modB"
                     /locus_tag="ACHY3M_002813"
     CDS             14179..14868
                     /gene="modB"
                     /locus_tag="ACHY3M_002813"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFS4317700.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14871..15929
                     /gene="modC"
                     /locus_tag="ACHY3M_002814"
     CDS             14871..15929
                     /gene="modC"
                     /locus_tag="ACHY3M_002814"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFS4317701.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15930..16748)
                     /gene="ybhA"
                     /locus_tag="ACHY3M_002815"
     CDS             complement(15930..16748)
                     /gene="ybhA"
                     /locus_tag="ACHY3M_002815"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFS4317702.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     HHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16903..17898
                     /gene="pgl"
                     /locus_tag="ACHY3M_002816"
     CDS             16903..17898
                     /gene="pgl"
                     /locus_tag="ACHY3M_002816"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFS4317703.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17939..18892)
                     /locus_tag="ACHY3M_002817"
     CDS             complement(17939..18892)
                     /locus_tag="ACHY3M_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4317704.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            19076..20127
                     /locus_tag="ACHY3M_002818"
                     /pseudo
     CDS             19076..20127
                     /locus_tag="ACHY3M_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
     gene            20203..21636
                     /locus_tag="ACHY3M_002819"
     CDS             20203..21636
                     /locus_tag="ACHY3M_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFS4317705.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWVLAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21819..24080
                     /locus_tag="ACHY3M_002820"
     CDS             21819..24080
                     /locus_tag="ACHY3M_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFS4317706.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24314..25597)
                     /locus_tag="ACHY3M_002821"
     CDS             complement(24314..25597)
                     /locus_tag="ACHY3M_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFS4317707.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVS
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25732..26808)
                     /locus_tag="ACHY3M_002822"
     CDS             complement(25732..26808)
                     /locus_tag="ACHY3M_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4317708.1"
                     /translation="MWCWTRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRR
                     IAITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     THREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         6388 a         6618 c         6944 g         6860 t
ORIGIN      
        1 taaaaagcgc cctaaaggcg cttttttgct atctgcgata cttaaagatt cgaacctgca
       61 gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc
      121 gagcgaagcg agtcatcctg cacgacccac caatgtaaaa aagcgcccta aaggcgcttt
      181 tttgctattc aggcatcctc aatttcactt tgtaaacctg atgacatcgt cagagcttac
      241 tgtgcaagca actctacgtc ggtggaatta ggcgtaattc gagtgacttt tccccaccat
      301 tcgactatct tgtttagcat ataaaacaaa ttacaccgat aacagcgaat gttacgctaa
      361 tgtcggtttt aacgttaagc ctgtaaaacg agatggtaag atgagcgtaa tgtttgatcc
      421 agacacggcg atttatcctt tccccccgaa gccgacgccg ttaagcattg atgaaaaagc
      481 gtattaccgc gagaagataa aacgtctgct aaaagaacgt aatgcggtga tggttgccca
      541 ctactatacc gatcccgaaa ttcaacaact ggcagaagaa accggtggct gtatttctga
      601 ttctctggaa atggcgcgct tcggtgcaaa gcatcccgct tctactttgt tagtcgctgg
      661 ggtgagattt atgggagaaa ccgccaaaat tctcagtccg gaaaaaacaa ttctgatgcc
      721 gacacttcag gctgaatgtt cactggatct cggctgccct gttgaagaat ttaacgcatt
      781 ttgcgatgcc catcccgatc gtactgtcgt cgtctacgcc aacacttctg ctgcggtaaa
      841 agcgcgcgca gattgggtgg taacttcaag cattgccgtc gaacttattg atcatcttga
      901 tagtttgggt gaaaaaatca tctgggcacc cgacaaacat ctggggcgtt acgtgcaaaa
      961 acagacgggt ggagacattc tatgctggca gggtgcctgt attgtgcatg atgaatttaa
     1021 gactcaggcg ttaacccgct tgcaagaaga atacccggat gctgccatac tggtgcatcc
     1081 agaatcacca caagctattg tcgatatggc ggatgcggtc ggttccacca gtcaactgat
     1141 cgctgctgcg aaaacattgc cacatcagcg gcttatcgtg gcaaccgatc ggggcatttt
     1201 ctacaaaatg cagcaggcag tgccagataa agagttactg gaagcaccaa ccgcaggtga
     1261 gggtgcaacc tgccgcagct gcgcgcattg tccgtggatg gccatgaatg gccttcagtc
     1321 catcgcagag gcattagaac aggaaggaag caatcacgag gttcatgttg atgaaaggct
     1381 gcgagagagg gcgctggtgc cgctcaatcg tatgctggat tttgcggcta cactacgtgg
     1441 ataacgaata ataaggcgta acgttacgct ttgggggaaa gatggatttt tttagtgtgc
     1501 agaatatcct ggtacatata ccaatagggg caggcggtta tgatctctca tggatcgaag
     1561 cggtaggcac gatcgccggg ttgctgtgta ttggccttgc cagtctggag aagatcagca
     1621 actacttctt tggcctgatc aacgtcacct tgtttggcat tattttcttt cagattcagc
     1681 tgtatgccag cctgctatta caggtgtttt tctttgccgc gaatatttac ggttggtatg
     1741 cgtggtcgcg acaaaccagt cagaacgagg cggagttgaa aattcgctgg ttgccattgc
     1801 cgaaggcact cagctggttg gcggtttgcg ttgtttcgat tggtctgatg acggtattta
     1861 tcaatccggt gtttgcattt ttgacccgcc tggcagtcat gatcatgcaa gcattaggat
     1921 tacaggttgt gatgcctgaa ctgcaaccgg acgctttccc gttctgggat tcatgcatga
     1981 tggtgttatc tatcgtggca atgattctga tgacgcgtaa gtatgtggaa aactggctgt
     2041 tgtgggtgat tattaacgtg attagcgtcg ttatttttgc acttcagggc gtttacgcca
     2101 tgtctctgga gtacatcatc ctgaccttta ttgcgctcaa cggcagccgg atgtggatca
     2161 acagcgcacg tgaaagaggc tcacgcgcgc tgtcccatta atggtgatga tgtgaatggc
     2221 cagacacccc tttattcagg tggcagtctg gcccatgaca aggttgatat tccatctgaa
     2281 tggtggcgtg ctcaatctga tagtgatcca tcaggtagtg ttggatctga tccaacaagg
     2341 catcgtgatc gtgtggcggg atcacctgca catgcaacgt catcaccggc ttctcgccta
     2401 ccatccatac atgtacatgg tgcacattgc ggacttccgg gatttcccgg cacatacgac
     2461 gcttcagttc agcgatatcc agcgataccg gtgcaccttc aagtaattca ttcacgctat
     2521 ctttcaacag tcgccacgcg ctacgcagaa ccagaagcga caccagtatc gagagaatgg
     2581 ggtcagcagg tgtccagcca gtccagataa taatcaacgc ggcaataata gccccaaccg
     2641 aacccagcag gtccccgagc acatgcagtg ccgctgctcg tacgttgagg tttttctctt
     2701 cgctgccgtg atgaagtaac cagaaagaaa gtatatttgc cagcaaccca gccacggcaa
     2761 ttgccatcat catgccgccc tcgaccggac gcggcgtgcg gaagcgttct atcgcctccc
     2821 agacaatcaa aatggtaatt accacgaggg ctatggcatt cacaaacgcc gcgagcgtgg
     2881 tcagtcttag ccaaccgaaa gtgtggcgaa tggtgggagg acgacgtgaa aattgcacgg
     2941 cgagtagggc gaaaagcagg gcggcagtat cggtcaacat atgacccgca tcggccagca
     3001 atgccagaga accagaaaga aaaccaccaa cgacttctac cagcataaac ccggcagtca
     3061 cgccgaaagc atacaacaag cgacgagcat tattatcttc tggcaggtgt gaggacgtgt
     3121 gtgagtgtga gtgcgccatg acgagtcatc ccttaattat tctcacttaa ctttatgaca
     3181 tcatactgct tttagaagtg aaaaattaaa agggagagac tccgctctcc cattattggc
     3241 tattttgcag ggttactgcg tggtaccgtc ggttttggta tcgacatcat tattgatgcc
     3301 atcaccggtt tgtacctttt tattgatatc cggacagcga ccatctttgc acatggtgtt
     3361 cttgtgctct tcatctttgg tcattccgtc attgttcatt gaagaaccat ccgaatgcag
     3421 cattgtgcca ccagaaccgg tatttacccc gttattgtcg acgttatttg gcgcgacatt
     3481 ttcacgggcg tcaggggcta cctggcccgc atcagctgcg gcgtttgcct ggccgttatt
     3541 agtttgcgct ccgctatcgg cagccagtgc ggcaccgctg gcaaggctta gagtggcagt
     3601 cagaaataat gtggccagtt ttgtcatttt cataggatgc tcctgttatg gtcgttatgt
     3661 cggataacct cttccaacag tgcatttgca ggtgaatata aggcattggt ttaagatttc
     3721 agccaggtta tgaaacgcag cagagaatct tgaaataatt aacaaacaaa ggagttacag
     3781 ttagaaattg taggagagat ctcgtttttc gcgacaatct ggcgtttttc ttgctaattc
     3841 caggattaat ccgttcatag tgtaaaaccc cgtttacaca ttctgacgga agatatagat
     3901 tggaagtatt gcattcacta agataagtat ggcaacactg gaacagacat gaattatcag
     3961 aacgacgatt tacgcatcaa agaaatcaaa gagttacttc ctcctgtcgc attgctggaa
     4021 aaattccccg ctactgaaaa tgccgcgaat acggttgccc atgcccgaaa agcgatccat
     4081 aagatcctga aaggtaatga tgatcgcctg ttggtggtga ttggcccatg ctcaattcat
     4141 gatcctgtcg cagcaaaaga gtatgccact cgcttgctgg cgctgcgtga agagctgaaa
     4201 gatgagctgg aaatcgtaat gcgcgtctat tttgaaaagc cgcgtaccac ggtgggctgg
     4261 aaagggctga ttaacgatcc gcatatggat aacagcttcc agatcaacga cggtctgcgt
     4321 atagcccgta aattgctgct tgatattaac gacagcggtc tgccagcggc gggtgagttt
     4381 ctcgatatga tcaccccaca atatctcgct gacctgatga gctggggcgc aattggtgca
     4441 cgtaccaccg aatcgcaggt gcaccgcgaa ctggcgtctg gtctttcttg tccggtaggt
     4501 tttaaaaatg gcacagacgg tacgattaaa gtggctatcg atgccattaa tgccgccggt
     4561 gcgccgcact gcttcctgtc cgtaactaaa tgggggcatt cggcgattgt gaataccagc
     4621 ggtaacggtg attgccatat cattctgcgc ggcggtaaag agcctaacta cagcgcgaag
     4681 cacgttgctg aagtgaaaga agggctgaac aaagcaggcc tgccagcaca ggtgatgatc
     4741 gatttcagcc atgctaactc gtccaaacaa ttcaaaaagc agatggatgt atgtgctgac
     4801 gtttgccagc agattgccgg tggcgaaaca gccattattg gcgtgatggt ggaaagccat
     4861 ctggtggaag gcaatcagag cctcgagagc ggggagccgc tggcctacgg taagagcatc
     4921 accgatgcct gcatcggctg ggaagatacc gatgctctgt tacgtcaact ggcgaatgca
     4981 gtaaaagcgc gtcgcgggta aggtttaatt gtcggatgcg ccgtcagagt ggcgtatccg
     5041 atgaatcacc acaggcctga taagacgcgc agcgtcgcat caggcaatgt gctccattgt
     5101 tagcaacaaa aaagccggct cacttgcagt cggctttctc atttttaaac gaatgacgtt
     5161 tacttcgctt taccctggtt agcaaccgcc gctgctttcg ctgcgatctc gtcagcatta
     5221 cccagataat agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg
     5281 ccggtcggga tattcagctc aagaatctct tcttcactca tgttatcaag atatttcacc
     5341 agcgcacgta aagagttacc gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc
     5401 ggtagaatag tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc
     5461 gtcagcggca gttctttctc gctcagcttc gcgtaacgtg gatcgtgacc cggataacgc
     5521 tcatcatctt tagtcagttc cggcggagtg actgcaaaac cacgacgcca ctgtttcacc
     5581 tgctcgtcgc catacttttc agcggtttct gctttgttca gaccctgcaa cgcaccgtag
     5641 tgacgttcgt tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc
     5701 agcacattcc acagggtatg gatagcgcgt ttcagcacag aagtgtaagc aaagtcaaag
     5761 ctgtaacctt cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca
     5821 gacagatcca cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca
     5881 tgacgaacca gaaccagctt agttacagcc atatacttac tcctcaaatc atcttttaat
     5941 gataataatt ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata
     6001 gcgaaaatag tggcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt
     6061 ttgctggtga tttgaacaat atgaagataa agccctcatg acgagggctt aacattactc
     6121 agcaataaac tgatattccg tcaggctgga atactcttcg ccaggacgca ggaagcagtc
     6181 cggttgcggc cattcagggt ggttcgggct gtccggcaga aactcgcttt ccaacgccag
     6241 cccttgccag tcggcgtaag gttcggttcc ccgcgacggt gtgccgccga ggaagttgcc
     6301 ggagtagaat tgaagagccg gagcggtggt gtagaccttc agctgcaatt tttcatctgc
     6361 tgaccagaca tgcgccgcca ctttcttgcc atcgcctttg gcctgtaaca agaatgcgtg
     6421 atcgtaacct ttcactttgc gctgatcgtc gtcggcaaga aactcactgg cgatgatttt
     6481 ggcgctgcgg aaatcaaaag acgttccggc gacagatttc aagccgtcgt gcggaatgcc
     6541 gccttcatca accggaagat attcgtccgc cagaatctgc aacttgtgat tgcgcacgtc
     6601 agactgctcg ccgtcaagat tgaaatagac gtgattagtc atattcaccg ggcaaggttt
     6661 atcaactgtg gcgcgataag taatggagat acggttatcg tcggtcagac gatattgcac
     6721 cgttgcgccg agattacccg ggaagccctg atcgccatca tctgaactca gggcaaacag
     6781 cacctgacga tcgttctggt tcacaatctg ccagcgacgt ttgtcgaacc cttccgggcc
     6841 gccgtgcagc tggttaacgc cctgacttgg cgaaagggtc acggtttcac cgtcaaaggt
     6901 ataacggcta ttggcgatgc ggttggcata acgaccaata gaggccccca gaaacgcggc
     6961 ctgatcctga tagcattccg ggctggcaca gccgagcagc gcctcgcgga cgctgccatc
     7021 ggaaagcgga atacgggctg aaagtaaagt cgcaccccag tccatcagcg tgactaccat
     7081 ccctgcgttg ttacgcaaag ttaacagtcg gtacggctga ccatcgggtg ccagtgcggg
     7141 agtttcgttc agcactgtcc tgctccttgt gatggtttac aaacgtaaaa agtctcttta
     7201 atacctgttt ttgcttcata ttgttcagcg acagcttgct gtacggcagg caccagctct
     7261 tccgggatca acgcgacgat acagccgcca aatccgccgc cggtcatgcg tacgccacct
     7321 ttgtcgccaa tcacagcttt gacgatttct accagagtgt caatttgcgg cacggtgatt
     7381 tcgaaatcat cgcgcataga ggcatgagac tccgccatca actcgcccat acgtttcagg
     7441 tcgccctgct ccagcgcgct ggcagcttca acggtgcggg cgttttcagt caggatatgc
     7501 cgcacgcgtt tcgccacgat tgggtccagc tcatgtgcaa cagcgttgaa ctcttcaatg
     7561 gtgacatcgc gcagggctgg ctgctggaag aaacgcgcac cggtttcgca ctgttcacga
     7621 cgggtgttgt attcgctgcc aaccagggta cgtttgaagt tactgttgat gatgacgaca
     7681 gccacacctt tcggcatgga aactgctttg gtccccagtg agcgacagtc aatcagcaag
     7741 gcatgatctt tcttgccgag tgcggaaatt agctgatcca tgatcccgca gttacagcca
     7801 acaaactggt tttctgcttc ctgaccgtta agcgcgattt gtgcgccgtc cagcggcaga
     7861 taataaagct gctgcaatac ggttccgacc gcgacttcca gtgaagcgga agaacttaac
     7921 ccggcaccct gcggcacatt gccgctgatc accatgtcca caccgccgaa gctgttgtta
     7981 cgcagttgca gatgtttcac cacgccacga acgtagttcg cccattgata gttttcatgc
     8041 gcgacaatgg gcgcattgag ggaaaactcg tcgagctgat tttcataatc ggctgccatt
     8101 acgcgaactt tacggtcatc gcgtggcgca cagctgatca cggtttgata atcaatcgcg
     8161 cagggcagaa cgaaaccgtc gttgtagtcg gtgtgttcac caatcaaatt cacgcggcca
     8221 ggcgcctgaa tggtgtgagt ggcagggtag ccaaatgcgt tggcaaacag agattgtgtt
     8281 ttttctttca gactcatttc ttacactccg gattcgcgaa aatggatatc gctgactgcg
     8341 cgcaaacgct ctgctgcctg ttctgcggtc aggtctcgct gggtctctgc cagcatttca
     8401 taaccaacca taaatttacg tacggtggcg gagcgcagca gaggcggata aaagtgcgcg
     8461 tgcagctgcc agtgttgatt ctcttcgcca ttaaatggtg cgccgtgcca gcccatagag
     8521 taggggaagg agcactggaa gaggttgtca taacgactgg tcagcttttt caacgccagc
     8581 gccaaatcgc tgcgctgggc gtcggtcaaa tcggtgatcc gcaaaacgtg ggctttgggc
     8641 agcagtagcg tttcgaacgg ccaggcagcc cagtaaggca caacggctaa ccagtgttcg
     8701 gtttcgacaa cggtacggct accgtctgcc agctcgcgct gaacataatc caccagcatt
     8761 ggtgatttct gttcggcaaa atattctttt tgcaggcggt cttcgcgctc agcttcgtta
     8821 ggcaggaagc tatttgccca aatctgtccg tgcggatgcg ggttagagca gcccatcgcc
     8881 gcacctttgt tttcaaagac ctgcacccac gggtacgttt ttcccagctc tgcggtttgc
     8941 tcctgccagg ttttgacgat ttccgtcaat gccgcaacgc tcagttctgg caacgtttta
     9001 ctgtgatccg gtgaaaagca gatcacccgg ctggtaccgc gcgcgctctg gcaacgcatc
     9061 aaaggatcgt tgctttctgg cgcatctggc gtgtcagaca tcaaggccgc aaagtcatta
     9121 gtgaaaacgt aagtcctggt gtaatcgggg tttttatcgc ctgtcacccg cacattacct
     9181 gcgcagagga aacaatctgg atcgtgcgca ggtaacacct gtttggctgg cgtttcctgc
     9241 gccccctgcc aggggcgctt agcgcggtgc ggtgaaacca gaatccattg cccggtgagc
     9301 gggttgtagc ggcgatgtgg atgatcaacg ggattaaatt gcgtcatggt cgttccttaa
     9361 tcgggatatc cctgtggatg gcgtgactgc cagtgccagg tgtcctgcgc catttcatcg
     9421 agtgtgcgcg ttacgcgcca gttcagttca cggtcggctt tgctggcgtc cgcccagtag
     9481 gccggaaggt cgccctcgcg acgcggtgca aaatgataat taaccggttt gccgcaggct
     9541 ttgctgaagg cattaaccac gtccagcacg ctgttgccta cgccagcgcc gaggttgtag
     9601 atgtgtacgc ctggcttgtt cgccagtttt tccatcgcca cgacgtgacc gtccgccaga
     9661 tccattacgt ggatgtaatc gcgtacgcca gtaccatctt cggtcggata atcgttacca
     9721 aaaatcgcca gcgagtcgcg acggcctaca gcaacctggg cgatgtatgg catcaggtta
     9781 ttcggaatgc cttgcggatc ttcgcccata tcgcccgacg gatgcgcgcc aaccgggttg
     9841 aagtagcgca gcagggcaat gctccagtcc ggctgggctt tttgcagatc ggtgaggatc
     9901 tgttccacca tcagcttgct tttgccgtaa gggctttgtg gtgtgccggt cgggaagctt
     9961 tcaacgtatg gaattttggg ctgatcgcca taaacggtgg cggaggagct aaaaataaag
    10021 tttttgacgt tagcggcgcg catggcgcta atcaggcgca gagtgccgtt gacattgttg
    10081 tcgtaatatt ccagcggttt ttgtaccgat tcgccgacgg ctttcagccc ggcgaagtgg
    10141 atcacggtgt cgatagcgtg atcgtgcagt atctcggtca tcaacgcttc gttacgaata
    10201 tcgccttcaa caaacgttgg atgtttgccg cctaaacgct cgataacagg cagtacgctg
    10261 cgcttactgt tacagaggtt atcaagaatg atgacatcat gaccgttttg cagtaattgc
    10321 acacaggtat gacttccaat gtaaccgcta ccaccggtaa ccagaactct cataattcgc
    10381 tccattaggc ttatggtatg aaataaccat agcataacaa agatgcgaaa agtgtgacat
    10441 ggaataaatt agtgaaatcg tttacacaag aatttagccg ttttttatgc gcgattaagt
    10501 gattataaaa cagagggttt atgaatgatt gcgcttttta tctgaaaaaa gaagcggttt
    10561 catgcctgca tgcgtcgaac cgttggccgg agagggcgtt taggccgcct ccggcaaggt
    10621 cagcactacc gactcaatat atttttgtca gcacatagcg atagagtcca ccgtccggca
    10681 cgaactcaag gcgatgggta atacaggcag gcgcatcttc agcgtggtgc gaaacaaaca
    10741 acaattgcgt ttcaccttcg ctaatcagca catcaacaaa acggcggata agctggcgat
    10801 tgagcggatc aagcccctgt agtggttcat cgagaataag caacgtcgga tgtttcacca
    10861 gtgcgcggac gatcagcgcc agacgctgct gtccccagga aagactatgg aacggagcgt
    10921 cagccgtacg tttatcaatg ccgagaatat ccagccactg ctgcaccagt ttttgctggc
    10981 gatccgaaac ggcctgataa atgccaatcg aatcaaaata gccagaaaga atcacattac
    11041 gcacggtagt gctgacccgg taatccagat gcaaactact gctgacgtaa ccgatatgct
    11101 ttttgatatc ccagatggtt tcgccgctgc cgcgacgtcg tccgaaaagc gtcaaatcgt
    11161 tgctgtaacc ttgcggatga tcgccagtaa ccaggcttaa taacgtcgat tttcctgcac
    11221 catttggccc gacaatttgc cagtgttcac ctggattcac ctgccagcta aggttattaa
    11281 gaatggggcg atcgttataa gaaaccacgc cattgttcag cacaatgcgc ggttcgttgg
    11341 cgggtaaggc gtgacgtgct gaaggttcat ccggctccgg cagttgcaca ccttcaagct
    11401 gttcactatg cgccagttgc gcgacgagtg cttgttggag cagttcctct ttagcgccag
    11461 tttccgctaa cgtgcaatcc gccagcacgc cagcaaactg gacaaactcc gggatctcat
    11521 cgaagcgatt gagcaccagt accagagtaa taccggactg atgtaacgag gcgagtcgct
    11581 cagccagctg ctgacgtgag gcaacatcca ggccatcgaa cggctcatca agaatcaaca
    11641 agtcaggctc cgacatcagc gcctgacaca gcagggtttt tcgcgtctcg ccagtggaaa
    11701 ggtatttaaa gcgtcggtcg aggagggcgg taataccgaa ctgctgcgcc agttgcatgc
    11761 aacgcggtgc atcctttact tcatcctgaa tgatctcagc cgtagtgcgt ccggtgtcat
    11821 cttcgccagg gccgagcata tcggtgttat tccgctgcca ttcgtcgctg acgagttttt
    11881 gcaattgctc gaaggagaga cgagtgatgt gggaaaactg gctttgccgt tcacctttca
    11941 aaagcggaag ttcccccgcc agcgcgcggg ccagggccga tttcccgctt ccattcgaac
    12001 cgacaaacgc ccaactatca cccgcgttta acgttagctg aggcaattgc agcgtttttg
    12061 tgtcgctaag acgaaacgtg ccttgcaaaa tttgcaacga tgacatttta tatcccattt
    12121 tgtgcagcga ttactgacag ggatacgtgt ttcataacaa attgtcaaca cgcttagcac
    12181 agcgtggcga taatcacgct gtcggcatta aagtaggccg tgacattctg cccttgctga
    12241 agagaagtcg cttcatttac cggcactgtg gcgcacagtg tttgcccgtc gggtagcgcc
    12301 attaatactt cgcactgctc tgcgccgcgc tcaatatgac taataatacc cggtaattgg
    12361 ttgtcggcgt tttgtgcgac cgcctcgtcc tgagtaatac ctacccacgg cgcttttagc
    12421 aatatcaaca cttctttgcc ttcatccagc cccagacgcg cgccgctttg tgcggtaatt
    12481 gcgactttca ggcgtgtttt tccgtcagcc agtaagacat caacatgctg ttgaacgtca
    12541 tcatgatcgc gggcggtgat ggtgccgaac cactggttac gggcgctggt ttgcagtgaa
    12601 aaacgtgaga tcgcggccag caggctgttc agcggcaggg cgtcatcgtc acttaacaca
    12661 tcaaaggctt tttgctggat ttgcgccagt aagtcataga gctgaatcag tcgctgacca
    12721 tagcgggtca gtactgcgcc gccgccacct ttaccgcctg ttgcgcgctc gaccagaata
    12781 tgctcactta actgattcat ctcgttaatg gcatcccagg cgcttttata gctgataccg
    12841 gcatctttcg ctccctggct aatggaaccg gaaagcgcaa tgtgttttag tagcgaaatc
    12901 cggcgcgggt cggcgaataa tttttgttgg agcttaaggg tgagaaggat ttcggcctgc
    12961 ataacaatgt cctggcaaaa gtcttattgt gacggaaaac gaacgccacg caaagctgac
    13021 cgcacaaaag gggagtgctt ttctgtgctt agcggttaga atagtctcat gactatatct
    13081 ggagttgacc atgttagagt tattaaaaag tctggtattc gccgtaatca tggtacctgt
    13141 cgtgatggcc atcatcctgg gtctgattta cggtcttggt gaagtattca acatcttttc
    13201 tggtgttggt aaaaaagacc agcccggaca aaatcattga ttccctgaat gcccgcttag
    13261 tcgggcattt ctttttctca acttcctgct tttccagccg atattttttc ttatctacct
    13321 cacaaaggtt agcaataact gctgggaaaa ttctgagcta gccgttatat tgtcgcctac
    13381 ataacgttac attaaggggt taccaatggc tcgtaaatgg ttgaacttgt ttgccggggc
    13441 ggcactctct ttcgctgttg ctggcaatac actggcagat gaagggaaaa tcacggtgtt
    13501 cgccgccgca tcactgacta acgcaatgca ggacattgct acgcagtata aaaaagaaaa
    13561 aggcgtggat gtggtttctt ctttcgcttc gtcatctact ctggcccgtc agattgaagc
    13621 gggagcgcct gcggatctgt ttatctctgc cgatcagaaa tggatggatt atgcggttga
    13681 taaaaaagcg atcgatacgg cttcgcgcca gacactgctc ggcaatagcc tggtcgttgt
    13741 agcaccgaaa gccagcgagc agaaagattt caccatcgac agcaaaacca actggacttc
    13801 gctgctgaat ggcggtcgcc tcgcggttgg cgatccggaa catgtccctg ctggcattta
    13861 tgcgaaagaa gcactgcaaa aactgggcgc atgggatacg ctctccccga aactggctcc
    13921 ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc aatgaagcgc cgttgggcat
    13981 tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa gtggttgcca ccttcccgga
    14041 agattcacat aaaaaagtgg aatatccggt tgctgttgtg gaagagcata acaatgcgac
    14101 agtgaaagca ttttatgatt atttgaaagg gtcgcaggct gcggaaatct ttaaacgtta
    14161 cggatttaca accaagtaat gatactgacc gatccagaat ggcaggcagt tttattaagc
    14221 ctgaaagttt cttccctggc tgtgctgttt agcctgccgt ttgggatctt ttttgcctgg
    14281 ttactggtgc gttgcacgtt tccgggcaaa gctctgctcg acagcgtact gcatctaccg
    14341 ctggtgttac cgcccgtggt cgtcggttac ttattattag tttcgatggg acggcgcgga
    14401 tttatcggtg aacgtctgta tgactggttt ggtattacct tcgcctttag ctggcgcggc
    14461 gcggttctcg ctgccgccgt catgtcgttt ccgctgatgg tgcgggcaat tcgtctggcg
    14521 ctggaagggg ttgatgtcaa actcgaacag gccgcaagaa cactgggggc cgggcgctgg
    14581 cgcgttttct ttactatcac gttaccgctg accttaccgg gaattattgt tggtacggta
    14641 ctggcttttg ctcgttctct cggtgagttt ggtgcaacca tcacctttgt gtcgaacatt
    14701 cctggtgaaa cgcgaaccat tccttctgcc atgtataccc tgatccagac ccccggcggg
    14761 gaaagtggcg cggcgagact gtgcattatt tctattgcgc tggcgatgat ctccctgttg
    14821 atttcagaat ggctggccag aatcagccgt gaacgggcgg ggcgctaatc atgctggaac
    14881 tgaatttttc ccagaccttg ggcaaccatt gcctgaccat taatgaaacg ctgcccgcca
    14941 atggcatcac tgctatcttt ggcgtctccg gtgccggaaa aacttcgctg attaacgcca
    15001 tcagtggact gacgcgcccg caaaaaggga ggattgtcct caatggtcgg gtactaaatg
    15061 atgccgaaaa aggtatctgc ctgacgccag aaaagcgtcg cgttggctat gtttttcagg
    15121 atgcgcggct gtttccgcat tacaaagtgc gtggcaatct gcgctacggc atggcgaaaa
    15181 gcatggtcga tcagttcgat aagctggtgg cgcttttagg cattgaaccg ttgcttgacc
    15241 gtttaccagg cagcctgtcc ggaggcgaaa aacagcgcgt agcgattggg cgggctttgc
    15301 tgacggcacc agaattgctg ttgctggatg aaccgctggc gtcactggat attccgcgta
    15361 aacgcgaact gttgccttat ctgcaacgtc tgacgcggga aatcaacatc ccgatgttgt
    15421 atgtcagcca ttcgctggat gagatcctcc atctggcaga cagagtgatg gtactggaaa
    15481 acggtcaggt gaaagccttt ggcgcactgg aagaagtctg gggtagtagc gtgatgaatc
    15541 cgtggctgcc gaaagagcaa caaagtagca ttctgaaagt gacggtactt gagcatcatc
    15601 cgcattacgc gatgactgcg ctggcgctgg gcgatcagca tttgtgggtc aataagctgg
    15661 acgaaccgct gcaagctgcg ctacgtatcc gcattcaggc ttccgatgtt tctctggttt
    15721 tacaaccgcc gcagcaaacc agcattcgta acgtattgcg ggcaaaagtt gttaatagtt
    15781 atgacgacaa cggccaggtg gaagtggaac tggaagtcgg aggtaaaacg ctgtgggcgc
    15841 gtatcagccc gtgggccagg gatgaactgg tgatcaaacc tggcctgtgg ctgtacgcgc
    15901 aaattaaaag tgtgtcgata accgcctgat taaatcaggt ggctataaat gaactgggca
    15961 atgctgtcgg tggtgttatc accaatcaca atgttggcgc gcgcttttac cgcgtcatcg
    16021 gcgttgccca tcgccacgcc tgtaccagcg gcctccagca tactgatatc attaaagtta
    16081 tcgccgaacg ccacgacgtt ttccatcgac cagccttgcg cctcaaccca tttcgtcaaa
    16141 cgtttacctt tgctgttgcc gccgcgtgca atatcaacct gatcgtgcca ggaccattca
    16201 cactccagtc ccagttcatg ttcgacatgc ttaccaaaat gctgcaattg cggcaggtca
    16261 tcgtgcgtca gggcgaactt ccatacggcg ttaacttgtt gcgccgtttc agccagagaa
    16321 gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg tttgcgccca gttagacgtg
    16381 cgaatgacat gcccggtcgg gtgctcatag accattgcat catccacata catcagaccg
    16441 tgaatgtggt gttcattcag catctcaatg agttgcaggg ctttattaac gggcattggg
    16501 tccgcttcca gcacggtttt tgcatgataa tcatacaaat aggtgccatt acagcaaata
    16561 gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa tagcgacgtg atggtgacct
    16621 gtgacgatga ttaattgata gcctgcttcg cgagcgcggg ccagggcttc tatcgatgaa
    16681 ggaagcaggg tctttttcgg ggtcaataag gtgccgtcta agtcgagagc aatcacgcgt
    16741 gtggtcatgg cgtattccag attaaggtta agaattttcg tctgcgcgaa tggtacaccg
    16801 ataccactat cggacaaaat tctgcgtttt aattcagcat tcaccgccaa aagcgactaa
    16861 ttttagctgt tacagtcagt tgctaaatgc aaaggagcat tcatgaagca aacagtttat
    16921 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
    16981 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
    17041 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tagtatcgcc
    17101 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
    17161 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
    17221 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
    17281 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
    17341 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctcgt ggcgcaggac
    17401 cctgcggaag tgaccaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
    17461 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
    17521 gatccgcacg gtaatatcga atgtgtccag acgctggata tgatgccgga aaacttctcc
    17581 gacacccgtt gggcggctga tattcatatc accccggatg gtcgccattt atacgcctgc
    17641 gaccgtaccg ccagcctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
    17701 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
    17761 aagtatctga ttgccgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
    17821 gagcagggac tactgcatga aaaaggccgt tatgcggtcg ggcagggacc aatgtgggtg
    17881 gtggttaacg cacactaacc gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt
    17941 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
    18001 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
    18061 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
    18121 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
    18181 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
    18241 ccagcttcca gggcttgtcg gctagctgtt gttctatcaa caatcggttg ccgctggagg
    18301 aacgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
    18361 ccaatggatg gtcacgccga caggccagca cgaatggctc gttgaccagt ggggtaaaat
    18421 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
    18481 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
    18541 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
    18601 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
    18661 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
    18721 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
    18781 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcagcattg ttaaatgaac
    18841 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
    18901 cggaaaatgg aagcgacgat tttgggtggc tggtcgttaa aaattttaac tgcatttagc
    18961 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
    19021 gtccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
    19081 aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc tgttagcgga
    19141 acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta tgggttccgg
    19201 taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta aagtcgccat
    19261 tattagccgt tccagcgatc cgcgtgctga tgttgattat ctgtttgctc aagttatcgt
    19321 ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctttctg gcgttggggc
    19381 attcgccatt gaaaatggtt tgattgcagc gacatcaccg gtcacccgtg tgcgtatccg
    19441 caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga
    19501 gtacgagggt agtgccagaa ttgacggcgt accgggtact gccgcaccgg ttgcgctcac
    19561 tttcctgaat gccgctggaa ccaaaaccgg aaaagttttc ccgactgata atcagattga
    19621 ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg tcattattcc
    19681 ggctgaatat ctgggaaaaa caggttatga attaccggcg gaactggatg ccgacaaagc
    19741 attattagcc cgcattgaat ctatccgtct acaagcgggt aaagcaatgg gcttaggtga
    19801 tgtcagtaat atggtatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
    19861 attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
    19921 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
    19981 gtaggatacg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
    20041 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacgac cagaaaaata
    20101 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caagataagg ataatcaata
    20161 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
    20221 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
    20281 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtggggctg
    20341 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctccatggtg
    20401 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
    20461 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
    20521 ggtttaggta aacgtattgc ctatctgctg attggtaaaa tcggtaacac cacgctgggt
    20581 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
    20641 gcgcgtgcgg gcggtattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc
    20701 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
    20761 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
    20821 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggtact ggccgccgga
    20881 ttgccgggca tcattatgct gctggtcacc ccactggtga tttataccat gtatccgcca
    20941 gaaattaaga aggtggataa taaaaccatc gctaaagcgg gccttgccga actgggaccg
    21001 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
    21061 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
    21121 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
    21181 ttaatctggt acggcggtat tatcggatta agctccttat tatcgaaagt taaattcttc
    21241 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
    21301 ttcttcgtta ttattttcct cagcattatc gtgcgttatt tcttcgcttc cggtagtgcc
    21361 tatatcgttg ctatgttacc ggtatttgcc atgctggcga acgtctccgg cgcaccgtta
    21421 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
    21481 ggcggcgcgg caggtccggt catctttggc gtgggttata acgatattaa atcctggtgg
    21541 ttggtcggtg cggtactgac gatattaacc ttcctggtgc atatcaccct cggcgtgtgg
    21601 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat tctcgtcata cttcaagttg
    21661 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt
    21721 cactcgcttg tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt
    21781 ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag
    21841 gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta
    21901 aaaaagaaga agccaaaaaa ggcactattg cctggtctat tctctcttcg cataatacgt
    21961 ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct
    22021 ttgttggtat tgtacagacc gctaaagcgt ccggtatgga acgtttcccg ctgccgtatg
    22081 tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg cactattaac ggtgatgacc
    22141 atgtttttgg tttatcggcg gcccagcgtt atggcggtat ttttgtgcct ccgcatattg
    22201 cggtcatcca tcaatatatg cgtgagatga tggcaggcgg cggcaaaatg atcctcgggt
    22261 cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag ggcggcggtg
    22321 agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg
    22381 tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg ctggctatca
    22441 ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg
    22501 gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca
    22561 cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg
    22621 gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca
    22681 tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg
    22741 tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg
    22801 agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg
    22861 gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg
    22921 tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg
    22981 cagtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag
    23041 atttgattgg cgcaggcgca atcatcagaa ccgcgttctg cggcccatgc tttggcgcgg
    23101 gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc
    23161 gcgaaggctc taagccagct aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt
    23221 ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct
    23281 gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg
    23341 gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc
    23401 cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg
    23461 aagtgaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc
    23521 cgattggtct ggcggagttt accctgtcac gccgcgatcc cggttatgtt ggcagaagta
    23581 aagcgactgc tgagctggaa aatcagcgtc tggcggggaa tgtcagcgag ctgacagagg
    23641 tgtttgcgcg cattaagcag attgctggtc aggagcatat tgatccgctg caaactgaaa
    23701 ttggcagcat ggtatatgcg gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga
    23761 gctgccagcg tgtgattggc ggtctggcga atattgccga agagtacgcg actaaacgct
    23821 accgttctaa cgtcatcaac tgggggatgt taccgctgca gatggcggaa gtgccaacct
    23881 ttgaagtggg ggattacatt tacatccctg gcattaaagc ggcgctggat aatccgggta
    23941 cgacgtttaa aggttatgtg atccatgaag atgcgccggt aacggaaatt acgctctata
    24001 tggaaagtct gactgctgag gagcgcgaga ttatcaaggc gggtagtttg attaacttca
    24061 ataaaaaccg tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg gcacttattg
    24121 tcggatgcga tgcttgcgca tcttatccga cctacgaatc gcatcgaatc tgtaggccag
    24181 ataaggcatt ttcgcagcat ccggcactta ttgtcggatg cgatgcttgc gcatcttatc
    24241 cgacctacaa atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa
    24301 tagttaattg ctcttacttc ttcgcctctg caaccacttt actacccacg ccgcggttat
    24361 tgtattccca catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat
    24421 catctacgct gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg
    24481 gtttagccgt gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg
    24541 tattggcatc aacatccagc gagcggccca gttgcgccac accatcaccg aaagcattga
    24601 aacggctgtt tacggcgagg aaaccgtagt aaatgttgga cagcgtagcc ggtgcaaaca
    24661 catacgcttc ttgctgagta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca
    24721 ctgcgccgcg accagaaacg atatccacat ccccttcaat gtagctgttg ctcaccagcg
    24781 tacgcggctg acgattcgtt tccagacggt tctgcacacc gctgttggtg acaaagaagg
    24841 tgttctgacg accgagaatg ttaacgttgt taatctgtac ctggtcacca tcagtacgca
    24901 gtgccaccgc cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca
    24961 gattttgcag ttgcaggcca ttgttttgtg accagaagac cgcagagcag agaacaccga
    25021 tactgtcgct gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca
    25081 tatatttgcc gcgcgggttg acgtcgtgac gccagtcggc agggctcatg ccaccatcaa
    25141 gggaaagccc aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtaattccac
    25201 ccggagcggc agggacatat accgttccct gatactcacc aggcatcacg gcaatatact
    25261 ggcgcttgtt ggtacgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg
    25321 ttacaccttg agtgcccgcc gggccgacaa caaagtcagg ttgcgcaggc agggtaatcg
    25381 gggaaggatt ccacgctgct gcacctggtg tcagggatgc aaaatagtgt tgagcatcga
    25441 aattctgcgc ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcggtttgat
    25501 cagaaggacg ttgatcgggc ggggttgagc tacaggcggt cagcgtcacg ccaaaagcca
    25561 atgccagcgc cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata
    25621 gaaaaatgaa tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt
    25681 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgat
    25741 ttcaattttg tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga
    25801 cttatgcccg agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc
    25861 agacaaactg cgcaactcgt gaaaggtcgg cggatcccct tcgaaggaaa gacctgatgc
    25921 ttttcgtgcg cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgatgagt
    25981 agatgcaatt atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat
    26041 tgatatcccg agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt
    26101 gctttgctcg acataaagat atccatctac gatatcagac cacttcattt cgcataaatc
    26161 accaactcgc tgcccggtaa caacagccag ctccattgca agtcttagcc aacatggtga
    26221 tgattctgct gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac
    26281 ctctgatttt gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat
    26341 tgcctctcgg aatgcatcgc tcagtgttga tctgattaac ttggctgacg ccgccttgcc
    26401 ctcgtctatg tatccactga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg
    26461 agcatcaggc agccccctcc ttattgcttt aattttgctc atgtaattta tgagagtctt
    26521 ctgcttgatt cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt
    26581 aacagaatta tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa
    26641 ctcaatgttg gcctgtattg cttcagtgat tgctatcctc ctgtctcggc ctaatccaaa
    26701 ctctttaccc gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaagatt
    26761 agggggtaaa tcccggcgct catgacttcg cctcgtccag caccacatcc
//
LOCUS       DAZUFB010000059        26682 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZUFB010000059 DAZUFB010000000
VERSION     DAZUFB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.059"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1196
                     /locus_tag="ACHY3M_002823"
     CDS             <1..1196
                     /locus_tag="ACHY3M_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFS4317709.1"
                     /translation="YFLKSLSPTLDYHEGPIGKLPFKGKLKAQVLENVVNLIAISEED
                     WNTEETSWGFKKHPITKLDNLNYLQASFEAFKNDVQETIIRTQQLEEFNNKEIIREYG
                     LESELNYKVSLEEITLTVNPDYKYSGTTDDKSKRMQSDSLSSLLSYAIGCMMGRYSLD
                     REGLVYAHEGNKGFADLVAEGAYKTFPADSDGILPLMDDEWFDDDVTSRVKEFVRTVW
                     GEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHLKMYKKRPIYWLF
                     SSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQLDEASGGEATR
                     LKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGDLLADVKAITGN
                     APEVI"
     gene            1373..3970
                     /gene="pglZ"
                     /locus_tag="ACHY3M_002824"
     CDS             1373..3970
                     /gene="pglZ"
                     /locus_tag="ACHY3M_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS4317710.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHESQLAVKKRIEIDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDEASLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKHHGLDSVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADPQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQTALHPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDRETFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEVVKPQFQNPQIKRVFVIVSDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVANTAGVSDNSEFLIPKGIQRFHFSGGARFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNNTMEKRVREVTLKLIGANFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            3981..6065
                     /gene="brxL"
                     /locus_tag="ACHY3M_002825"
     CDS             3981..6065
                     /gene="brxL"
                     /locus_tag="ACHY3M_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS4317711.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARRHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPAAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            6184..7533
                     /locus_tag="ACHY3M_002826"
     CDS             6184..7533
                     /locus_tag="ACHY3M_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HFS4317712.1"
                     /translation="MKLIANGLNKQFFSSFLPPPDTEIDGVVAAIAYGDDKTSLLDHC
                     IKNHHRLDIWMRYDHTVPVSPAFLSKLLANVKNNIFCKLVPDCLHCKIIWWKGYGAYI
                     GSANLTGRAWYSNIEAGVFYDENDLYNTGLIEQLEEFFDNLSDLDSCIELTKEIIQEQ
                     QQLQKLKLEQEKKEQAIIKKRLIPEWAGVSNYDKIKSSDKRKDAFRKEWESTLSTIRN
                     ISSQINDFRPAWISEDTPAFWQTDQFLHAYYYNQVRRNDKTFPYEEDYQNNRKNPQAA
                     LMNMLSWWKSLSEPPSHEDINLGINANYIREHLAKDKINTLSEEELHKIFSYTHATMD
                     HVIKMSVDTFGLTSRISLDKEKRAILFTQWIMKQTNKNGMTIAELLNYVLYDGKQELM
                     WERIYLAGKDDNYKFQHYGINSISEVVGWARPEVTPPRNGRTNKALRALGYPVKIYI"
     gene            complement(7709..8029)
                     /locus_tag="ACHY3M_002827"
                     /pseudo
     CDS             complement(7709..8029)
                     /locus_tag="ACHY3M_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8113..8886
                     /locus_tag="ACHY3M_002828"
     CDS             8113..8886
                     /locus_tag="ACHY3M_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFS4317713.1"
                     /translation="MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIK
                     LRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLSSLSWQGRSHYLQLKQNQCWLSV
                     ENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
                     AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRID
                     TSKINLGSGNRQIVVNGKLNTQYQITVPENFTKKGISHG"
     gene            8879..9796
                     /locus_tag="ACHY3M_002829"
     CDS             8879..9796
                     /locus_tag="ACHY3M_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4317714.1"
                     /translation="MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPR
                     LSVDIDLAWIPLESREVALPNVREALTRIAAELEAQTGIRAVLQTHNPDEMRIIVTTD
                     DAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
                     PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMT
                     FEEVSLEELKEVPERMLSTLKTHFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQW
                     KLRNIGQMSEEKRAQSLEKLEKVMTEWLR"
     gene            9875..10257
                     /locus_tag="ACHY3M_002830"
                     /pseudo
     CDS             9875..10257
                     /locus_tag="ACHY3M_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830158.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10310..10972)
                     /locus_tag="ACHY3M_002831"
     CDS             complement(10310..10972)
                     /locus_tag="ACHY3M_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317715.1"
                     /translation="MDKILDIVNGWITAAVASEVLQIKQILLFIFIAGPLALVIWKIR
                     EIINFVNEVRNSKLNELQHLLDSHKLSDEVNICVEDEITRIIGYRMTGIADVSRQKII
                     LPLLIKNRDSIPVDFFKKFKSFLMIENGTLIFKKGFSFWVENIIYGWFSLQFLLLAVL
                     SLVLSIYRHEQIPVWGHLVLYFVAAILFLFFIAFARLIPRPEECNLLNNLLQTQYNNS
                     SE"
     gene            complement(11045..11686)
                     /locus_tag="ACHY3M_002832"
                     /pseudo
     CDS             complement(11045..11686)
                     /locus_tag="ACHY3M_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(11887..11971)
                     /locus_tag="ACHY3M_002833"
     tRNA            complement(11887..11971)
                     /locus_tag="ACHY3M_002833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11935..11937),aa:Leu,seq:caa)
     gene            12167..13186
                     /gene="ahr"
                     /locus_tag="ACHY3M_002834"
     CDS             12167..13186
                     /gene="ahr"
                     /locus_tag="ACHY3M_002834"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFS4317716.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDTGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVSTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSEQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            13316..14818
                     /gene="yjgR"
                     /locus_tag="ACHY3M_002835"
     CDS             13316..14818
                     /gene="yjgR"
                     /locus_tag="ACHY3M_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFS4317717.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAIIQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(14937..16019)
                     /gene="lptG"
                     /locus_tag="ACHY3M_002836"
     CDS             complement(14937..16019)
                     /gene="lptG"
                     /locus_tag="ACHY3M_002836"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS4317718.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(16019..17119)
                     /gene="lptF"
                     /locus_tag="ACHY3M_002837"
     CDS             complement(16019..17119)
                     /gene="lptF"
                     /locus_tag="ACHY3M_002837"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS4317719.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(17182..17232)
                     /gene="ytgA"
                     /locus_tag="ACHY3M_002838"
     CDS             complement(17182..17232)
                     /gene="ytgA"
                     /locus_tag="ACHY3M_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFS4317720.1"
                     /translation="MSIIILATISTTQENK"
     gene            17386..18897
                     /gene="pepA"
                     /locus_tag="ACHY3M_002839"
     CDS             17386..18897
                     /gene="pepA"
                     /locus_tag="ACHY3M_002839"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFS4317721.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            19030..19473
                     /gene="holC"
                     /locus_tag="ACHY3M_002840"
     CDS             19030..19473
                     /gene="holC"
                     /locus_tag="ACHY3M_002840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFS4317722.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            19473..22328
                     /gene="valS"
                     /locus_tag="ACHY3M_002841"
     CDS             19473..22328
                     /gene="valS"
                     /locus_tag="ACHY3M_002841"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4317723.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     KDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(22384..23580)
                     /gene="yjgN"
                     /locus_tag="ACHY3M_002842"
     CDS             complement(22384..23580)
                     /gene="yjgN"
                     /locus_tag="ACHY3M_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFS4317724.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            23773..24276
                     /gene="yjgM"
                     /locus_tag="ACHY3M_002843"
     CDS             23773..24276
                     /gene="yjgM"
                     /locus_tag="ACHY3M_002843"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4317725.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(24322..24738)
                     /gene="rraB"
                     /locus_tag="ACHY3M_002844"
     CDS             complement(24322..24738)
                     /gene="rraB"
                     /locus_tag="ACHY3M_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFS4317726.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            24900..25904
                     /gene="argF"
                     /locus_tag="ACHY3M_002845"
     CDS             24900..25904
                     /gene="argF"
                     /locus_tag="ACHY3M_002845"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4317727.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYN
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(26005..26235)
                     /locus_tag="ACHY3M_002846"
     CDS             complement(26005..26235)
                     /locus_tag="ACHY3M_002846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317728.1"
                     /translation="MKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKTNEIVKN
                     NPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
     gene            complement(26222..>26682)
                     /locus_tag="ACHY3M_002847"
     CDS             complement(26222..>26682)
                     /locus_tag="ACHY3M_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4317729.1"
                     /translation="AKVNLTPVIDSILEIFLKFPYINDVRILDWCFNKRMQYFGDSEK
                     IKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEHYKNSRKLQELIWDKLV
                     AYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFFEGFFHEAT"
BASE COUNT         7244 a         6454 c         6514 g         6470 t
ORIGIN      
        1 attatttttt gaaatcatta tcaccaacgc ttgattacca cgaaggtccg ataggtaaac
       61 ttccttttaa aggaaagctt aaggctcaag ttttagaaaa tgttgtaaat ttaatcgcta
      121 tttcagagga agactggaat actgaggaaa catcttgggg gtttaaaaaa caccctatca
      181 caaaattaga caacctgaat tatttgcagg ctagttttga agcttttaaa aatgatgtac
      241 aagaaactat aattagaacc caacagcttg aagagttcaa taacaaggaa ataattagag
      301 aatatggact agaatcagag ctaaactata aagtttcttt agaagaaata acattaacgg
      361 taaatcctga ttataagtat tctggaacca cggatgataa atcaaaaagg atgcagtcag
      421 acagtctttc ctccctacta tcctatgcca tcggctgcat gatgggccgc tactccctcg
      481 atcgcgaagg ccttgtctac gcccatgaag gtaacaaagg cttcgccgat ctcgtcgccg
      541 aaggtgccta caaaaccttc ccggccgaca gtgacggtat cctgccgctg atggatgacg
      601 agtggtttga cgacgacgtc acctctcgcg tcaaagagtt tgtccgcacc gtctggggcg
      661 aagagcacct gcaggaaaac ctcgaattta tcgccgaaag tctctgttta tacgcgatca
      721 agccaaaaaa aggcgaatct gcgctggaga ccattcgccg ctatctttcc acccagttct
      781 ggaaagatca tctgaaaatg tacaaaaagc gccctatcta ctggctgttt agctccggta
      841 aagagaaagc cttcgagtgc ctggtgtatc tgcatcgcta taacgacgcc acgctgtcga
      901 gaatgcgtac cgaatatgtg gtgccgctgc tggcgcgtta tcaggccaat atcgatcgcc
      961 tgaacgatca acttgatgag gcttcaggcg gtgaagctac tcgcctgaaa cgcgaacgcg
     1021 atagcctgat caaaaaattc agcgaactgc gcagctatga cgaccgcctg cgtcactatg
     1081 ctgatatgag aatcagtatt gatctcgacg atggcgttaa ggttaactac ggcaagtttg
     1141 gcgatctgct ggcagatgtt aaagccatca ccggcaatgc cccagaggtg atctaaacca
     1201 gacggcgcgt tctcctgctg ccgggttctg cccggtggca aataccaccg ggaaacgcgc
     1261 cgctgctggc atttctccac ctcacttcat gataaaatgc gccatcgtgt caaaatctcc
     1321 ttttcacgtt ttggcgcttt cttattcatc gtaacaacat gggattgtga acttgcaaaa
     1381 tcaggacttt attgctggcc ttaaagctaa atttgccgaa catcgcatcg ttttctggca
     1441 cgatcccgat aaacgtttta ttgaggaact ggaacagctc aagcttgaaa acgtcacgct
     1501 aatcaacatg acccacgagt cacagctggc ggtgaaaaaa cgcatcgaga ttgatgagcc
     1561 agaacagcag ttcctgctgt ggttccccca tgatgcgccg cctcatgaac aagactggct
     1621 gctggatatc cgcctttaca gcagcgaatt ccatgccgat tttgccgcca tcaccctgaa
     1681 cacgctgggc attccccagc ttggcctgcg cgagcatatt cagcgacgca aggccttctt
     1741 cagcactaaa cgcacgcagg cgctgaaaaa cttggtgaca gaacaggaag atgaagcctc
     1801 gctggataag aaaatgattg cggtgatcgc tggcgcaaag accgcgaaaa ccgaagacat
     1861 tttgttcaat ctgattaccc agtacgttaa ccaacaaaca gaagacgaca gcgaactgga
     1921 aaacacgcag gcgatgctga aacaccacgg tctggactcg gtattgtggg aaatgctcaa
     1981 ccacgaaatg ggctaccagg cagaggagcc atcgctggaa aacctgctcc tgaaactgtt
     2041 ttgtaccgat ctctctgccc aggccgaccc acagcagcgc gcctggctgg aaaaaaatgt
     2101 cctgctgacg ccatccggca gagcatccgc cctggcattt atggtgacct ggcgtgccga
     2161 tcgtcgctat aaagaggctt atgactactg cgctcagcaa atgcagaccg ccctgcaccc
     2221 ggaagatcat taccggctta gctcgccata tgatttgcac gaatgcgaaa ccaccctcag
     2281 catcgaacaa accattattc atgcgctggt aacgcagctg ctggaagaga gcaccacgct
     2341 cgatcgggaa acctttaaaa aattgctctc tgaacgccag agcaaatact ggtgccagac
     2401 acaacctgag tattacgcca tctatgacgc gctgcgccag gcagagcgtt tgctgaacct
     2461 gcgcaatcgc cacatcgatg gtttccacta ccaggacagc gccaccttct ggaaagccta
     2521 ctgcgaagaa ctgttccgct tcgaccaggc ttatcgcctg tttaatgaat atgccctgct
     2581 ggttcacagc aaaggagcga tgatcctcaa gagcctggat gattatatcg aggcgctcta
     2641 cagcaactgg tatctggcag agttaagccg taactggaac gaagtgctgg aggcggaaaa
     2701 tcgtatgcag gcgtggcaaa tccctggcgt gccgcgtcag cagaactttt tcaatgaggt
     2761 ggtgaagccg cagttccaaa atccgcaaat caaacgcgtg ttcgtgatag tttccgatgc
     2821 cctgcgttat gaagtggcgg aggagctggg gaatcaaatc aataccgaga aacgctttac
     2881 cgcagaactg cgatcgcagc taggcgtgct cccaagctac acccaactgg gaatggcggc
     2941 gttgctgcct catgaacaac tttgttatca accgggtaac ggtgacattg tttatgctga
     3001 tgggctgtcg acctcgggta ttcctaaccg cgataccatt ctgaaaaact ataagggaat
     3061 ggcgataaaa tcgaaggacc ttctggagtt aaaaaatcag gaaggacgag accttattcg
     3121 cgattacgaa gtggtgtata tctggcataa cacgattgat gccaccggcg acacggcatc
     3181 cacggaagat aaaaccttcg aagcgtgccg cacggcggtg actgaactga aagatttagt
     3241 caccaaagta atcaaccgcc tccacggcac acgcattttt gttacggcgg atcacggttt
     3301 cctgttccag caacaggcgc tttcggttca ggataaaaca actctgcaaa ttaagccgga
     3361 aaacaccatc aagaaccaca aacgctttat tatcggccat cagcttcccg ccgatgattt
     3421 ttgctggaaa ggtaaagtgg caaataccgc aggtgtgagc gacaacagcg agttcctgat
     3481 tccgaaaggg atccagcgct tccatttctc tggcggcgcg cgctttgttc atggcggcac
     3541 catgttgcag gaggtttgcg ttccggtatt gcagataaaa gccctgcaaa aaaccgccgc
     3601 agaaaaacag ccgcagcgcc gcccggtgga tattgtcgct taccatccga tgattaagct
     3661 ggtgaataat atcgataaag tgagcctgtt gcagacgcat ccggtgggcg aactttatga
     3721 accgcgtatc ctgaacattt acattgtcga caacgccaac aatgtggtct cgggcaaaga
     3781 gcgcatcagc tttgacagcg ataacaacac catggaaaaa cgcgtgcgcg aagttaccct
     3841 gaagctgatt ggcgctaact tcaaccgtcg caatgagtac tggttgatac tggaagacgc
     3901 acaaacggaa acggggtatc agaagtaccc ggtcattatc gatctggcat tccaggatga
     3961 tttcttctaa gtgaggcgat atgcaaaccc atcatgattt acctgtttca ggcgtatccg
     4021 caggggaaat tgcctccgag ggttacgatc tggacgccct gctgaaccag cattttgctg
     4081 gtcgcgtggt gcgtaaagat ctcaccaagc aactcaagga aggggcaaac gtcccggtgt
     4141 atgtgcttga gtatctgctc ggcatgtact gcgcctctga cgatgacgac gtagtcgagc
     4201 aagggttgca aaacgttaag cgtattctgg ctgataacta tgtgcgcccg gatgaagcag
     4261 agaaagtgaa gtcgctgatc cgcgagcgtg gttcgtacaa aatcatcgat aaagtgtcgg
     4321 tgaaactgaa ccagaaaaaa gacgtttacg aagcccagct ttctaacctc ggcatcaaag
     4381 acgcgctggt gccatcacag atggttaaag acaacgagaa gctactgacg ggcggtatct
     4441 ggtgcatgat taccgtcaac tatttctttg aagaagggca gaagacttcg cccttctcat
     4501 tgatgacgct taagcctatc cagatgccga atatggatat ggaagaagtg ttcgatgcgc
     4561 gtagacactt taaccgtgat cagtggatcg atgtgctgct gcgctcagtg ggtatggagc
     4621 ccgccaatat tgagcaacgc actaaatggc accttatcac ccgaatgatc ccgtttgtgg
     4681 agaacaacta taacgtttgc gaactggggc cgcgcggcac cggtaaaagc catgtgtata
     4741 aagagtgttc tcctaactcc ctgttagttt ctggcgggca aacgaccgtt gccaacctgt
     4801 tctacaacat ggccagtcgc cagatcggcc ttgttggcat gtgggatgtg gtagcgttcg
     4861 acgaagtcgc ggggatcact ttcaaagata aagacggcgt gcaaatcatg aaagattaca
     4921 tggcgtcagg atctttctct cgcggcagag attcgattga aggtaaagcg tcgatggttt
     4981 tcgtcggcaa catcaatcaa agcgtagaga cactcgttaa aaccagccat ttgctggcgc
     5041 catttccggc tgcgatgatt gataccgcct ttttcgaccg ctttcatgcc tatattcccg
     5101 gttgggaaat ccccaaaatg cgcccggaat tctttaccaa ccgttacggg ctgattacgg
     5161 attatctcgc tgaatatatg cgcgaaatgc gcaaacgcag tttctctgat gcgattgata
     5221 aattctttaa gctgggtaat aaccttaacc agcgtgacgt cattgctgtt cgacgtaccg
     5281 tgtcgggatt gttaaaactc atgcatcccg atggcgcgta cagcaaagaa gatgtgcgag
     5341 tctgcctgac ctatgcgatg gaagttcgtc gccgcgtgaa agagcaactt aaaaaactgg
     5401 gcggcctgga gttcttcgat gtgaacttta gctacatcga taacgaaacg ctggaagagt
     5461 tttttgtgag cgtaccggaa cagggcggca gcgaacttat tcctgccgga atgccaaagc
     5521 cgggcgttgt gcatctggtc actcaggcag aaagcggcat gaccgggttg tatcgctttg
     5581 aaacacagat gactgccggt aatggtaagc atagtgtatc gggtctgggt tcaaatacct
     5641 ccgcgaaaga agctatccgc gtcggtttcg attacttcaa aggcaatttg aaccgggtaa
     5701 gcgcggccgc gaaattctcc gatcatgaat atcatcttca tgtcgttgaa ctgcataata
     5761 ctggccctag caccgcaacc agtcttgctg ctcttattgc tttatgttcg atattgctgg
     5821 caaaaccggt gcaggagcag atggtggtgt tgggcagtat gacgcttggt ggagtaatta
     5881 acccggtaca agatcttgct gccagtttac agctcgcctt cgacagcggc gcaaaacgag
     5941 ttctgttgcc gatgtcctcg gctatggata ttccaacggt tccggcagag ttatttacca
     6001 agtttcaggt gagtttttac tcagacccgg ttgatgcggt ttataaggcg ctgggagtga
     6061 attaacatat atcgagggat ggatcacatt cctcggtata tttgtcccag gattattctt
     6121 atttgatata atatcagttc taattaactt attatcaatg gaatatttct cggctaaata
     6181 acaatgaaac ttatcgctaa cggattaaat aaacaatttt ttagttcatt tttaccacct
     6241 cctgatacag aaattgatgg tgttgtcgct gccattgctt atggcgatga taaaacctcg
     6301 cttttggatc actgcataaa aaaccatcac aggctcgata tttggatgcg atatgatcat
     6361 actgttccgg tatcgcccgc attcttatca aaacttctcg ccaatgttaa aaacaatata
     6421 ttttgtaaac tcgtaccaga ctgcctgcac tgcaaaataa tttggtggaa aggatatggt
     6481 gcttatattg gctctgctaa tttaactggg cgcgcgtggt attcgaatat tgaagctggt
     6541 gtattttatg atgaaaatga tctgtataac acaggattaa tagaacaact tgaagagttt
     6601 ttcgacaact tatctgacct tgactcttgt atcgaactta ctaaagagat aattcaagaa
     6661 caacagcagt tacaaaaatt aaaacttgaa caagagaaaa aagaacaagc aatcataaaa
     6721 aaacgactta tacccgaatg ggctggtgta agtaactatg ataagattaa atcaagtgat
     6781 aaacgcaaag atgctttccg taaagaatgg gagagtacac tttcgacaat acgaaatata
     6841 tccagccaga taaacgattt caggcctgca tggatatcag aagatacccc agcattctgg
     6901 cagacagatc agtttttaca tgcgtattat tataatcagg ttcgtaggaa tgataagaca
     6961 ttcccttatg aagaggatta tcagaacaac aggaaaaacc cacaagcggc actgatgaat
     7021 atgctgtcat ggtggaaaag tctctctgag ccaccttccc atgaagatat caatcttggt
     7081 ataaatgcta actatattcg tgagcactta gcaaaggata agattaatac cttatccgag
     7141 gaagagttac ataaaatatt ttcgtataca catgcaacaa tggaccatgt gataaaaatg
     7201 agcgtagata cctttggatt aacgagtcgt atatccctag ataaagaaaa acgggcgatt
     7261 ttgtttactc agtggataat gaaacaaact aataaaaacg gtatgacgat tgcagaatta
     7321 ctgaactatg tactttatga tggaaagcaa gagctcatgt gggaaagaat atatctggca
     7381 ggaaaagatg ataactataa gtttcagcat tacggaatca actcaatatc cgaagttgtc
     7441 ggctgggcgc gtcctgaagt gacaccgccc agaaatggga gaactaataa ggcgttaaga
     7501 gctttaggtt accctgttaa aatttatatc taaaaacact atataactta acttgtaaca
     7561 cctgctttgt taaaacaggt gttacagaga agcaaagatc aaataagata tttatcttcg
     7621 agatgcttca ttttgtgatt tattacttta ggaggaatgg tatataaacc gtatataaca
     7681 acgaatgccc aaattaaaac taactcggcc agccaaataa aacacgtgac cacaaatcaa
     7741 ttaacactgc cagatacagc cagccttcat ctgtgcgtaa gtaagtgaag tcctccgccc
     7801 acttctggtt cgggccactg acgtaaaaat catgctctag acacaggcag ttcgtgtgcg
     7861 cggtacctga cagggctgaa attccggcag gcttttgcgc tctgctcctg acggcgcagg
     7921 cttgtagcca cggtttttac attgaagtgg tattttgagc acgcagttaa tcagtcaagg
     7981 gggcaccata acgctgtttt aaccatgtaa aagcttcgtg gacaacgtta tgctacgaca
     8041 gacaccatac attgactttt tattcactaa atagtttact ttttttatta tagttacact
     8101 ctgagtgaat ctatggcatc aaaattaaac tggctgttgc aaaacacctc tccgggaagc
     8161 ctggtgttac aatcgtggct gacccggaac aacatatcac cttctctggc ttttaaatac
     8221 gcgcaaaatg gctggttaat aaaactgcgg gctggcgttt atgcgcgcgc aggacgagaa
     8281 ccagactgga gtgatgcgct atggtgccta caaaaccaac ttgaagcgcc ggtgcatctg
     8341 gccggactga gtagtctctc ctggcagggc cgttcccatt acctacaact taagcagaac
     8401 caatgctggc ttagcgttga aaacaaagcg atattgccaa aatggtttaa agaatttccc
     8461 gccgttgagt ggttgatggt atcggccctg aagctccctg acctggatga aaaataccga
     8521 actgagcttg aggttaaagg gaaaagaatt accgccagca cgccagaact ggctgcctat
     8581 gagctgttaa gcgcagtccc tcaggaaatc tcatttgagc acgctgccga gctttttcag
     8641 ggactagtca atctcaaccc acgtaaagtg gagaagctgc ttacactcag tcagtccgta
     8701 caaaccaagc gcctgtatct gttttttgcc agtttctatg aacatgcatg gttcaaacgc
     8761 atagatacca gcaaaatcaa cctgggatcg ggcaaccgac aaatcgttgt taatggcaag
     8821 ctgaacacgc aataccagat aactgtgccc gaaaatttta ctaagaaggg aataagccat
     8881 ggataaaacc tccccctact accgccaggt cgctctgttg gtgcgagcat taccctgggt
     8941 cgctaacgag tcctgttttg cgctgaaagg cggaacggcg atcaacttat tcatccatga
     9001 ttttccccga ttgtccgttg atatcgatct ggcctggata ccgctggaaa gcagagaggt
     9061 cgcgctgccc aacgtgcggg aggcgctcac ccgtatcgcc gcagaactgg aagcgcaaac
     9121 cgggatccgt gcagtgctgc aaacccataa tcccgatgag atgcgcatca ttgtcaccac
     9181 tgacgatgcg caaattaaaa ttgaagtctc gccagttgcc cgggggacac tatatcctcc
     9241 gcaagaacgc gatatcgtcg gagccgtgga ggaagaattt ggctttgccg cgctcccggt
     9301 ggtctcactg cccgatttat acggtggcaa gctatgtgct gcgttagatc gccagcaccc
     9361 cagagatttt tacgacgtca aaatgctcct tgatacacaa ggaattgatc gctcgatatt
     9421 caatggtttt atcgtgtact tgctcagcca taaccgccca ttatccgaag tgcttaaccc
     9481 acgttggaag gatattactg aaccctttca aaatgaattc agcggtatga cttttgagga
     9541 agtatcactg gaagagctaa aagaggtccc ggaaagaatg ctcagcacgt tgaaaacgca
     9601 tttcactcaa caggatttcg acttcctgat gtcatttaaa cgcggcgagc cagactggaa
     9661 tttagcgcca gagccccaca ttcagaattt gccagccgtt caatggaagc tgcgcaatat
     9721 tggacagatg tcggaggaaa agcgcgcgca gtcactggag aaactagaaa aggtcatgac
     9781 agaatggcta cgttagttta ataaattaag tatagttgca ccaatagtga aactatatca
     9841 ttttttataa attaacaaaa ccgtctcggg atccattgcc tttcagcact taaaaccagg
     9901 attgctattg ttcaactctc tcgcattgct tctgggcgta ctgtctggct tgttcctcgg
     9961 catatagatg ctggcaggct ctgctggtgc actaatgtgg ttaatagaac atctggcgct
    10021 tatcagcagt aaattgtagc aagggatcac gatgctgatt tcctcatcca atagtcagaa
    10081 taaattgttt tggacggagg cttcaccacc gtaatccacc gtgcgttgct gcctgtgggc
    10141 aatgttaccc tcacctttct gctgcgcctg gcgattctgt tacaggcgat gtcgattttt
    10201 catgtaccag agtatgtttt gataccagga actattctga ttgggatggc gatatagttg
    10261 tcgagtaggg cttccaaaaa gtatttatgt tcgccccctc atcaatgaat cattcggatg
    10321 aattattata ctgggtctgt agtagattat ttaataaatt acattcttca ggtcggggaa
    10381 tcaacctcgc aaaagcaata aagaacagga acaaaattgc agcaacaaag tataatacta
    10441 agtgccccca aacaggaatt tgctcatgcc tgtatataga taaaaccaaa gacaaaacag
    10501 caagtaataa aaattgcaat gaaaaccagc cataaatgat gttctcaacc caaaaagaaa
    10561 atcctttctt aaatataaga gttccatttt caatcatcaa aaaagatttg aatttcttga
    10621 agaagtcaac cggtattgaa tctctatttt ttattaataa cggcaaaata attttttgtc
    10681 tgctaacatc cgcaataccc gtcattcgat acccgattat ccttgtaatt tcatcttcta
    10741 cacaaatatt gacctcatcg gatagtttgt gactatccag tagatgctgc aattcattta
    10801 atttagagtt cctgacctca ttaacaaaat ttataatttc tcgtattttc cagataacaa
    10861 gagccaatgg tcctgcaata aaaataaaga gaagaatttg ttttatctgc aatacctcac
    10921 ttgctacagc agcagtgatc catccattca cgatatcaag tattttatcc ataacctatc
    10981 ccccaaaatt gaaactgtat atgaggataa aacagcataa tataaacgtg gtaatcgtat
    11041 tttcaatttc aggctgcaat cgctttaact ccagcgccgg acgatgctgt cgattactgg
    11101 aagcaaccgc ccccgccatc tcttgcgccg gattgtaatc aattaacccg ctttgcactg
    11161 cataacgcat gatggccgtg gtgcgctgtt gaaggcggga agccacttca agacgtccgg
    11221 atttctccac ggccttcata ggaattaaca gatcgcgggt gcctagttca gcaatgttgc
    11281 gagcaccaat tgccgggaag atattgtcct ccaggctctt ttttacacga ttagcatgat
    11341 cttccgacca tttttggttg gttacgagcc actctctggc aaccttctcg aaagtaataa
    11401 tctctttcgc ctgctcttct ttcacagcac gcttatgttc acgaggatcg ataccagcag
    11461 ctaccagctt tctggcctct tcccttttct tccgcgcatc cgccagcgaa acatccgggt
    11521 aaacaccgaa cgccatcaaa tgttgtttac cgccaaaacg gaaacgaaat cgccaatact
    11581 ttgaaccgcc aggttttaca agtaaggaca tgccgtcgcc gtcaacaagt gaatactctt
    11641 tctcctcaga tttcgcagag cgaactttag tatccgttag ggccataatg gtcttccttc
    11701 cattggtatc tcgtgagtat acaagcaata tcgaatcggc aaatatactc ataagtatac
    11761 tcacaagcct gttgatgtgg gttgattcag gttgacttca gttgaggaga aaagctggaa
    11821 aagccttgtg tagcgcggat ttcaggcata aaaaaagacc tcagttgagg tctatttgca
    11881 tacttttggt gccgaaggcc ggactcgaac cggcacgtat ttctacggtt gattttgaat
    11941 caactgcgtc taccgatttc gccacttcgg cactgaagag gtatgcggaa aacgttgtgg
    12001 attatacctg tcgcacgcca ccatgcaagc gccagcttgc tcgcacctcg ctaagtgctg
    12061 aaattttcag cattgcatac cgcgatgtgt aacctttgtc acactccagg caccccgccc
    12121 tgccatgctc tacacttccc agaccacacc agagaaggat aaaagcatgt cgatgataaa
    12181 aagctatgcc gcaaaagaag cgggcggcga actggaagtt tatgagtacg ataccggtga
    12241 gctgaagcca caagatattg aagtgcaggt ggattactgc gggatctgcc attccgatct
    12301 gtcgatgatc gacaacgaat ggggattttc acagtatccg ctggttgccg ggcatgaggt
    12361 gattgggcgc gtggtggcac tcgggagcgc cgcgcaggat aaaggtttgc aggtcggtca
    12421 gcgtgtcggg attggctgga cagcgcgtag ctgtggtcac tgcgacgcct gtattagcgg
    12481 taatcagatc aactgcgagc aaggcgcggt gtcaacaatt atgaatcgcg gtggctttgc
    12541 cgagaagttg cgtgcggact ggcaatgggt gattccactg ccagaaaata ttgatatcga
    12601 gtccgccggg ccgctgttgt gcggcggtat cacggtcttt aaaccactgt tgatgcacca
    12661 tatcactgct accagccgcg ttggggtaat tggtattggc gggctggggc atatcgctat
    12721 aaaacttctg cacgcaatgg gatgcgaggt gacggccttt agttctaatc cggcgaaaga
    12781 gcaggaagta ctggcgatgg gtgccgataa agtggtgaat agccgcgatc cgcaggcact
    12841 gaaagccctg tcggagcagt ttgatctcat tatcaatact gtgaacgtca gcctcgactg
    12901 gcagccttat tttgaggcgc tgacctatgg cggtaatttc catacggtcg gtgcggttct
    12961 cacgccgctg tctgttccgg cctttacgtt aattgcgggc gaccgcagca tctctggttc
    13021 tgctaccggc acgccttatg agctgcgaaa gctgatgcgc tttgccgccc gtagcaaggt
    13081 tgcgccgacc accgaactgt tcccgatgtc gaaaattaac gacgccatca agcatgtgcg
    13141 cgacggtaag gcgcgttacc gcgtggttct gaaagctgac ttctgatctc tttgccgggt
    13201 gcctgtcgcc cggcacttaa atctctttgg aaggcgtctc gcaatctgct taatttagcg
    13261 gtgaagagtg cacagccgtt gcctatactc caggcaggaa actggaggat atctcatgag
    13321 cgaacccctg ttaattgccc gcacgccgga cacagaactg tttttactgc cgggaatggc
    13381 taaccgtcac gggctgatta ctggcgcaac ggggacgggt aaaactgtca cgctgcaaaa
    13441 actggcggag tctctatcgg aaatcggtgt tccggtgttt atggccgatg tgaaaggcga
    13501 tctgaccggc atcgcgcagg caggaacggc atcggaaaaa ctgctcgctc ggttgaaaaa
    13561 tatcggtgtc aacgactggc aaccgcatgc caatccggtg gtggtgtggg atatctttgg
    13621 cgagaagggc catccggtgc gggcgacggt ttcggatctg gggccgctgt tgctggcgcg
    13681 gctgttgaat ctcaacgatg tgcaatctgg cgtgctgaat atcatcttcc gcattgctga
    13741 cgatcagggg ctgttactgc tcgactttaa agatttgcgg gcgattatcc agtacatcgg
    13801 cgataacgcc aaatctttcc agaatcagta cggaaatatc agcagcgcat cggttggtgc
    13861 catccagcgc gggctgttgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
    13921 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
    13981 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat
    14041 gatttcagag ttgtatgaac aattgccgga agcgggcgat ctggagaagc caaaactggt
    14101 gtttttcttc gacgaagcac atctgctgtt taacgatgca ccgcaggtac tgctggataa
    14161 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
    14221 aaacccgtct gatattccgg ataatgtgct cggccagctc ggtaatcgcg ttcagcacgc
    14281 tttgcgggct tttacgccca aagatcagaa agcggtaaaa gctgcggcgc aaaccatgcg
    14341 ggccaatccg gcgtttgata ccgaaaaggc gattcaggaa ctgggcaccg gcgaggcgtt
    14401 gatctctttt ctggatgcga aaggaagccc ttctgtggtg gagcgtgcga tggtgatcgc
    14461 gccttgttcg cggatggggc cggtgacgga agatgagcgt aatggcttga ttaatcactc
    14521 tccggtgtat ggcaaatatg aggatgaggt ggaccgggaa tccgcctatg agatgttgca
    14581 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgcgaaag ggaaagaggt
    14641 agcggtggat gacggcattc ttggtggatt gaaggatatt ttgttcggca ctaccggacc
    14701 acgcggcggg aagaaagatg gtgtggtgca aacaatggcc aaaagcgccg ctcgccaggt
    14761 gacgaatcag attgtgcgtg gaatgttggg gagtttgctg ggggggagaa gaaggtaagt
    14821 caggcgcgat ctgtccccct caccctaacc ctctccccaa aggggcgagg ggaccgatcg
    14881 aactcgtatt tgactttatc aacaatgtga aagggagctt tcgctccctt gcttggttac
    14941 gattttctca ttaacagcca caggctgatt aagaagaagc tggcgcttgg caacagtgcg
    15001 ccgatgatcg gcgggatgcc ataaaccaac gtcaacgggc cgaagatctg gtccagtacg
    15061 tagaagacaa aaccgaagct gataccggtg accacacgca cgcccatcgg tacgctacgc
    15121 agtgggccaa agatgaacga cagcgccatc agcatcatca ccgccacgga tagcggctgg
    15181 aagattttgc tccacatgtt gagctgataa cgtccagcgt cctgaccgct cgacttcaga
    15241 tacttcacgt agttgtgcaa accgctgatt gagagtgcat ccgggtccag cgccaccacg
    15301 cccagtttgt ctggcgtgag gttggttttc caggtgccgc tcaccgtctg cgaaccggtg
    15361 atctgtttcg gattggtcag atcagattca tcaacctgcg acagacgcca gactttatgt
    15421 tccgggtcaa acttcgcagt agcggcatag cgtacggatt gcagacgacg attttcgtta
    15481 aaggcataaa tgctgatgcc acctaactct tcgtcacctt taacccgctc aatgtagacg
    15541 aagttgttgc catctttcgc ccataagcct tgctgggtag agagcaacga gccgccgtac
    15601 atcgcctgcg cacggtagtt acgcgccatc tgctcgccct gcggcgcgac ccattcacca
    15661 atcgccatcg tcagcaagac tagcggaatg gcggttttca tcaccgacag cgcaacctgc
    15721 atacgggtga aaccagaagc ctgcatcacc accagttcgc tgcgctgcgc cagcatccca
    15781 agaccaagca acgccccaag cagagctgcc atcgggaaga agatttgcac atctttcggc
    15841 acgctcagca aggtatacat tcctgcgcct aacgcgtcgt aactcccctg cccggctttt
    15901 ttcagctgat cgacaaactt gataatgccc gacagcgaca ccagcatgaa cagcgtcatc
    15961 atgatggtgg tgaaaatagt tttaccgata tagcggtcaa gtacgccaaa aggttgcatc
    16021 acaccgctcc tttacgcgaa aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa
    16081 ggttgagaac aatcgccaaa gccagataaa tcaggttaac ggtccacatc cacagcgtcg
    16141 ggtccagctt acctttaccg ccgttcgatt tcagggaggt ctggatcagg aagaacaaca
    16201 gatacagcag catggctggc agcatcgata gtacgcgccc ctgacgcggg ttaaccacgc
    16261 tcagcggtac gaccataagt gccatcataa acacggtgaa caccaacgtg atacgccagt
    16321 tcagttctgc gcgagcacga tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt
    16381 cggtatcgtt cgggtcgagc gccaccgcct ggtgaccaat gatcgcctga taatcctgga
    16441 aatccgtaat gcggaaatca cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga
    16501 gagtgacgac ctgggagccg tcgcgcagct gggttaaatg tccggaatcg gccaccacca
    16561 cagaaggacg tgcattacct tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc
    16621 tgccgtcaac gctttcgatg aacagcaccg aactgccatt agtcgcttgc tggaattgcc
    16681 cttgcgccag cgccgccatg ccagggttcg ctttcgcttc tgctaacact tcatcctgat
    16741 gacgcgatga ccacggtccc gcccacatca cgttaaccgc cgcgacgatt gccgtgaata
    16801 ccgcaaggat cattgccgct ttcaccagaa ccgctttgct caggccgcag gcatgcatta
    16861 ccgtaatttc actttcggta tacagtttgc ccagcgtcat cagcagcccg aggaacaggc
    16921 ttaatggcag gataagctgc gccatttccg gcacgcccaa cccgagaagg gagagcacca
    16981 gattcgccgg aatatcgccg tcaaccgctg cgccgaggat cctcactaac ttttgacaga
    17041 agaagatcaa aagcaagatg aagagtatcg ccagctggct tttgagcgtc tcccgcacca
    17101 gatatcttat gattatcact ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta
    17161 gcttgtttca tggcttaaac gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat
    17221 aattatactc aacggattca cctctcagat tttgttctga cgtgccaatg ccgtaataac
    17281 gttaagatta acacgaagtc atcgcaacag cggacatgag ttacgaaagc ttgcaattct
    17341 atctgtagcc accgccgttg tctttaagat tcaggagcgt agtgcatgga gtttagtgta
    17401 aaaagcggta gcccggagaa acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca
    17461 cgtcgccttt ctccgattgc agaacagctc gataaaatca gcgatgggta catcagcgcc
    17521 ctgctacgtc ggggcgaact ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt
    17581 ccgaatgtac tttccgagcg aattctcctt attggttgcg gcaaagaacg tgagctggat
    17641 gagcgtcagt acaagcaggt tattcagaaa accattaata cgctgaatga tactggctca
    17701 atggaagcgg tctgctttct gactgaactg cacgttaaag gccgtaacaa ctactggaaa
    17761 gtgcgtcagg ctgtcgagac agcaaaagag acgctctaca gtttcgatca gctgaaaacg
    17821 aacaagagcg aaccgcgtcg tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt
    17881 gaactgacca gcggtgagcg cgcgatccag cacggtctgg cgattgccgc cgggattaaa
    17941 gcagcaaaag atctcggcaa tatgccgccg aatatctgta acgccgctta cctcgcttca
    18001 caagcgcgcc agctggctga cagctacagc aagaatgtca tcacccgcgt tatcggcgaa
    18061 cagcagatga aagagctggg gatgcattcc tatctggcgg tcggtcaggg ttcgcaaaac
    18121 gaatcgctga tgtcggtgat tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc
    18181 gtgctggtgg gtaaaggttt aaccttcgac tccggcggta tctcgatcaa gccttcagaa
    18241 ggcatggatg aaatgaagta cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg
    18301 atggtcgcgg agctacaact gccgattaac gttatcggcg tgttggcagg ctgcgaaaac
    18361 atgcctggcg gacgagccta tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc
    18421 gttgaagtgc tgaacaccga cgctgaaggc cgcctggtac tgtgcgacgt gttaacttac
    18481 gttgagcgtt ttgagccgga agcggtgatt gacgtggcga cgctgaccgg tgcctgcgtg
    18541 atcgcgctgg gtcatcatat tactggtctg atggcgaacc ataatccgct ggcccatgaa
    18601 ctgattgccg cgtctgaaca atccggtgac cgcgcatggc gcttaccgct gggtgacgag
    18661 tatcaggaac aactggagtc caattttgcc gatatggcga acattggcgg tcgtcctggt
    18721 ggggcgatta ccgcaggttg cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac
    18781 ctggatatcg ccggtaccgc ctggcgttct ggtaaagcaa aaggcgccac cggtcgtccg
    18841 gtagcgttgc tggcacagtt cctgttaaac cgcgctgggt ttaacggcga agagtaagtt
    18901 tgcgtttacc ctcaccctaa ccctctccct aagggagagg gaaccgatcg agcgcaatag
    18961 aaattcattg ctcatctttt agggtgagga atgcagcaat atttaaatcc accacaagaa
    19021 gccccattta tgaaaaacgc gacgttctac cttctggaca atgacaccac cgtcgatggc
    19081 ttaagcgccg ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag
    19141 cgcgtgctca tcgcctgtga agatgaaaag caggcttacc ggctggatga agccctgtgg
    19201 gcgcgtccgg cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgcggcggt
    19261 gcgccggtgg agatcgcctg gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt
    19321 agtctgcgaa caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt
    19381 ccttatgaag attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct
    19441 ggtttcaacc tgaatacggc aacctggaaa taatggaaaa gacatataac ccacaagata
    19501 tcgaacagcc gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg
    19561 aaagccagga aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata
    19621 tgggtcacgc cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg
    19681 gcaaaaacac cctgtggcag gtcggtactg accacgcagg gatcgctacc cagatggtcg
    19741 ttgagcgcaa gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt
    19801 tcatcgacaa aatctgggaa tggaaagcgg aatccggcgg caccattacc cgtcagatgc
    19861 gccgtctcgg caactccgtg gactgggagc gtgaacgctt caccatggac gaaggcctgt
    19921 ccaatgcggt gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt taccgtggca
    19981 aacgcctggt aaactgggat ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa
    20041 accgcgaatc gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa
    20101 ccgcagatgg taaagattat ctggtggttg cgactactcg tccggaaacc ctgctgggcg
    20161 atactggcgt agccgttaac ccggaagatc cgcgttacaa agatctgatt ggcaaatatg
    20221 tcattctgcc gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg
    20281 aaaaaggcac cggctgcgtg aaaatcactc cggcgcacga ctttaacgac tatgaagtgg
    20341 gtaaacgtca cgcgctgccg atgatcaaca tcctgacctt tgacggcgat atccgtgaaa
    20401 gtgcccaggt gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatcccgg
    20461 cagagttcca gaaactggag cgttttgctg cacgtaaagc agtcgttgct gcggttgacg
    20521 cgctcggcct gctggaagaa attaaaccgc acgacctgac cgttccttac ggcgaccgtg
    20581 gcggcgtagt tatcgaaccg atgctgaccg accagtggta cgtgcgtgcc gatgtcctgg
    20641 cgaaaccggc agttgaagcg gttgagaatg gcgacattca gttcgtaccg aagcagtacg
    20701 aaaatatgta tttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctgt
    20761 ggtggggtca ccgtatccct gcatggtacg acgaagcagg taacgtttat gttggccgca
    20821 acaaagacga agtgcgtaaa gaaaataacc tcggtgccga cgttgccctg cgtcaggacg
    20881 aagacgttct cgacacctgg ttctcttctg cgctgtggac cttctctacc ctaggctggc
    20941 cggaaaacac cgacgccctg cgtcagttcc acccaaccag cgtgatggta tctggtttcg
    21001 acatcatctt cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg
    21061 aaaatggcaa accgcaggtg ccgttccaca ccgtttacat gaccggcctg attcgtgacg
    21121 acgaaggcca gaagatgtcc aaatccaagg gtaacgttat tgacccactg gatatggttg
    21181 acggtatttc gctgccagaa ctgctggaaa aacgcaccgg caatatgatg cagccacagc
    21241 tggcggacaa aatccgtaag cgcaccgaga agcagttccc gaacggtatt gaaccgcacg
    21301 gtactgacgc gctgcgcttc accctagcgg cactggcgtc taccggtcgt gacatcaact
    21361 gggatatgaa gcgcctggaa ggttaccgta acttctgtaa caagctgtgg aacgccagcc
    21421 gctttgtgct gatgaacaca gaaggtcagg attgcggctt caacggcggc gaaatgacgc
    21481 tgtcgctggc ggaccgctgg attctggcgg agttcaacca gaccatcaaa gcgtaccgcg
    21541 aagcgctgga cagcttccgc ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga
    21601 accagttctg tgactggtat ctcgagctga ccaagccggt aatgaacggt ggcaccgaag
    21661 cagaactgcg cggtactcgc catacgctgg tgactgtact ggaaggtctg ctgcgcctcg
    21721 cgcatccgat cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg
    21781 gtatcactgc cgacaccatc atgctgcagc cgttcccgca gtacgatgca tctcaggttg
    21841 atgaagccgc actggccgac accgagtggc tgaagcaggc gatcgttgcg gtacgtaaca
    21901 tccgtgcaga aatgaacatc gcgccgggca aaccgctgga gctgctactg cgtggttgca
    21961 gcgcggatgc agaacgtcgc gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc
    22021 tggaaagtat caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt actaagatca
    22081 tcgacggtgc agagctgctg atcccgatgg ctggcctcat caacaaagaa gatgagctgg
    22141 cgcgtctggc gaaagaagtg gcgaagattg aaggtgaaat cagccgtatc gagaacaagc
    22201 tggcgaatga aggctttgtc gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaaa
    22261 agctggaagg ctatgcagaa gcgaaagcga agctgattga acagcaggct gttatcgccg
    22321 cgctgtaatc aaaacacagt aatgaaaacg aaggccggag catgctccgg ccttttttat
    22381 ctcttacaga aatggtaaag aaggcataat tccccgtgaa atcctggcta agaagccttt
    22441 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    22501 attttttgcc tgccagtcaa tcatccatat tttcagcagt ggataagcca gaccgcccgt
    22561 aatcccggat atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt
    22621 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    22681 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat
    22741 tatcatttta cgttgcattt ccataaattg ctgtaaattc tcaatatcat catttgcata
    22801 cacagaacta tcatacgcat ttaatatttg atcgaagata atataaccag caacagcgag
    22861 aaaaggtaat aaagctaaaa atgcgagaat ggcatatttt atacaatacg tagtatctaa
    22921 tttcaccttg aaacgatgta taccgaaact ggtattgctc cagagaaaac tcattaccag
    22981 gctatataaa gtaccattaa aaataccaat ggaaacaata ccaactattg ccatcagaac
    23041 cacagatata ataacactac ttgaactatt ggcatgtagc atctttgtcg agataaagaa
    23101 aacagtcccc atcccaatgg ccattaaaat cggcaagaaa aaggttaccc accagaaccc
    23161 tttcatagag caattaaaac taaatcttac gccgttgaga ctggtcatca aggcctgata
    23221 acgcagtcct tttgctgcca taaatataag caaaaccaac agtaacaaag tcaaaacaca
    23281 gccaacaagt ggcatatctg ctgacactgt cattaagatt gcgaaatagc aaaaaacaaa
    23341 aacaagacaa ctaacaaaaa cattcccacc agtaattcca taagaaaatc gttgtccgtt
    23401 aacttccata ttagcataga gataacgctt acatttcatt aatgcccatg gcaaatagat
    23461 acctagcgtt ataatcgtta acaacacatt caccacacaa ataagaaaat atctctcacc
    23521 tgtaccatga aaaacaaacg aatgggacgg aacatccatt tcattaataa cttgagccat
    23581 aaaaattccc tgaattaaaa aatccattga tataatttat atatacagac agtaaaatgt
    23641 tctgccacgt tacaatactc ttatcagtaa taggctggca aaaactttat tactttataa
    23701 gtcgcttgcg cacgtaagaa gagagtggta ttaaacaaat gtataattac tttattttta
    23761 ttgggttaaa ctatgaataa cattgcgccg caatcacctg taatgcgtcg cctgacgcag
    23821 caggataatc ccgccatcgc ccgcgtcatt cgtcaggtat ccgccgaata cggtcttacc
    23881 gctgataaag gctacaccgt cgccgatccg aacctggacg agttgtatca agtgtatagt
    23941 cagccgggtc atgcatattg ggtggttgag tacgatggtg aagtggtcgg cggcggtggg
    24001 attgcgccat taaccgggag tgagtcggac atttgcgaac tgcagaagat gtattttctt
    24061 ccggctattc gcggtaaagg gctggcgaaa aaactggcct taatggcgat ggagcaggca
    24121 cgagagatgg gcttcaaacg ctgctatctg gaaacgaccg cttttttgaa agaagcgatt
    24181 ggtttgtatg aacatttagg ttttcaacat atcgactatg cacttggctg cacgggccat
    24241 gtagattgtg aagtgcggat gctacgcgaa ctctaaaaaa atgcccgcta gtgtgtgcta
    24301 tcgggcattt taatcaatta attagtggcg aaccccgtca tcgtcttcat cgacaaaatc
    24361 ttcatcgtcg ccgtcttcgc cattgggatc ttcaaagtaa gtgccccaac cgtcgtactc
    24421 aacgtcaaat ttctccgcca gcgtcatcag ttgttcaacc tgggcatcga tcagatcggc
    24481 attcaacgcg cactcgctga ggatgtcgca gcaaatcacg atatcaccat cttcaacttc
    24541 cagctcttct ggatcggtca cttcgtaacc gagtttaaac gcttcaactg ctgctttttc
    24601 cagggtttcc agatcgtctg cggaaagatg gtgttcgatg gtgtacagcg cgtccggatc
    24661 gctgccatct tccagtaatt cttcaataat caaacgtgtt tcttcacgct gttcttccag
    24721 ttgttccggg tttgccatgg ctcattcctc ttaatgtcct gccgatactc ttattgtcac
    24781 acaccactga cattgcctcc acctttgtga caaagattta tgctttagac ttgcaaatga
    24841 ataatcatcc atataaattg aattttaatt cattaaggcg ttagccacag gagggatcta
    24901 tgtccgggtt ttatcataag catttcctga aattactcga tttcacgcca gctgaactca
    24961 acagcctgct gcagttagcc gcgaagctga aagccgataa gaaaagcggc aaagaagaag
    25021 ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa agactcgacc cgtacccgat
    25081 gctctttcga agttgccgca tatgaccagg gtgctcgcgt tacttatctc ggcccaagcg
    25141 gcagtcagat tggtcataaa gagtcgatta aagacactgc ccgcgtgctg ggtcgcatgt
    25201 ataacggtat tcagtatcgc ggctatggtc aggagattgt cgaaacactg gcggaatacg
    25261 ctggcgtgcc ggtatggaac ggcctgacca atgagtttca ccccacgcag ttgctggcgg
    25321 atctcctcac catgcaggag catttacccg gtaaagcatt caacgaaatg acgctggtct
    25381 atgcaggtga cgcacgtaac aacatgggca attcgatgct cgaagctgcg gcgcttaccg
    25441 gtctggattt gcgtctggtc gcgccacagg catgctggcc ggaagctgcg ctggttacgg
    25501 aatgccgcgc cctggcacag caaaatggcg ggaatattac gctgacggaa gatgtagcca
    25561 aaggagttga aggtgctgac tttatctata ccgatgtatg ggtgtcgatg ggggaagcaa
    25621 aagagaaatg ggcagaacgg attgcattac tgcgtgatta tcaggtgaac agcaagatga
    25681 tgcagttgac cggtaacccg gaggtcaaat tcctccactg cctgcccgcg tttcatgacg
    25741 accagacgac gcttggtaag aaaatggcgg aagagtttgg cctacatggc ggaatggaag
    25801 tgactgatga ggtcttcgaa tctgccgcca gcattgtttt tgatcaggcg gaaaaccgca
    25861 tgcataccat caaagcggtg atggtcgcga cgctcagtaa ataattgata tcgctataaa
    25921 gatgccggga aatacaaaaa catcccggca tcacattatt ttatgcagta atagttcaat
    25981 tattccatcc atatttttct attgctatac gtaaatcttc cggaggattt tcatcattct
    26041 tgttctaatt tttaattcat taaccatctt ttcttcaaaa atcttaacga cataacccgg
    26101 attgtttttt acaatttcat ttgttttttc ttcattacca taaacatatc ctaactcatt
    26161 aagatacagc tgttcgccct cataatcttt aagtaattga tagaaatacc acttagcaaa
    26221 atcaggttgc ttcatgaaaa aatccttcaa aaaaaatatc atttactaaa atgctgacag
    26281 gcacctcatt aaatagcaac ttaacatcat caaataacct tagtattaag cgtgattttt
    26341 cttgattgct taagttaaaa tcattgacat atgcaaccaa cttatcccaa atcaattcct
    26401 gtaatttacg gctattttta tagtgttctt tatcgagatt gaaaaataag gtctcagcta
    26461 ttttaatttt agattgatcg cagctaaaat ctttagattg atcgcggcta aaatcaatat
    26521 gatttatcac ggagcatgca tactttatct tttcagaatc accaaaatat tgcattcttt
    26581 tattaaaaca ccaatccagt attcttacat cattaatata tgggaatttt aaaaaaatct
    26641 ccagaatcga atcgatgacc ggtgtaaggt taacttttgc at
//
LOCUS       DAZUFB010000060        26155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZUFB010000060 DAZUFB010000000
VERSION     DAZUFB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.060"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..420
                     /gene="bfd"
                     /locus_tag="ACHY3M_002848"
     CDS             226..420
                     /gene="bfd"
                     /locus_tag="ACHY3M_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFS4317730.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            493..969
                     /gene="bfr"
                     /locus_tag="ACHY3M_002849"
     CDS             493..969
                     /gene="bfr"
                     /locus_tag="ACHY3M_002849"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFS4317731.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(966..1433)
                     /locus_tag="ACHY3M_002850"
     CDS             complement(966..1433)
                     /locus_tag="ACHY3M_002850"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4317732.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1812..2123
                     /gene="rpsJ"
                     /locus_tag="ACHY3M_002851"
     CDS             1812..2123
                     /gene="rpsJ"
                     /locus_tag="ACHY3M_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4317733.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2156..2785
                     /gene="rplC"
                     /locus_tag="ACHY3M_002852"
     CDS             2156..2785
                     /gene="rplC"
                     /locus_tag="ACHY3M_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4317734.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2796..3401
                     /gene="rplD"
                     /locus_tag="ACHY3M_002853"
     CDS             2796..3401
                     /gene="rplD"
                     /locus_tag="ACHY3M_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4317735.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3398..3700
                     /gene="rplW"
                     /locus_tag="ACHY3M_002854"
     CDS             3398..3700
                     /gene="rplW"
                     /locus_tag="ACHY3M_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4317736.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3718..4539
                     /gene="rplB"
                     /locus_tag="ACHY3M_002855"
     CDS             3718..4539
                     /gene="rplB"
                     /locus_tag="ACHY3M_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4317737.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4556..4834
                     /gene="rpsS"
                     /locus_tag="ACHY3M_002856"
     CDS             4556..4834
                     /gene="rpsS"
                     /locus_tag="ACHY3M_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4317738.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4849..5181
                     /gene="rplV"
                     /locus_tag="ACHY3M_002857"
     CDS             4849..5181
                     /gene="rplV"
                     /locus_tag="ACHY3M_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4317739.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5199..5900
                     /gene="rpsC"
                     /locus_tag="ACHY3M_002858"
     CDS             5199..5900
                     /gene="rpsC"
                     /locus_tag="ACHY3M_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4317740.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5913..6323
                     /gene="rplP"
                     /locus_tag="ACHY3M_002859"
     CDS             5913..6323
                     /gene="rplP"
                     /locus_tag="ACHY3M_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4317741.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6323..6514
                     /gene="rpmC"
                     /locus_tag="ACHY3M_002860"
     CDS             6323..6514
                     /gene="rpmC"
                     /locus_tag="ACHY3M_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4317742.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6514..6768
                     /gene="rpsQ"
                     /locus_tag="ACHY3M_002861"
     CDS             6514..6768
                     /gene="rpsQ"
                     /locus_tag="ACHY3M_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4317743.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6933..7304
                     /gene="rplN"
                     /locus_tag="ACHY3M_002862"
     CDS             6933..7304
                     /gene="rplN"
                     /locus_tag="ACHY3M_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4317744.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7315..7629
                     /gene="rplX"
                     /locus_tag="ACHY3M_002863"
     CDS             7315..7629
                     /gene="rplX"
                     /locus_tag="ACHY3M_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4317745.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7644..8183
                     /gene="rplE"
                     /locus_tag="ACHY3M_002864"
     CDS             7644..8183
                     /gene="rplE"
                     /locus_tag="ACHY3M_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4317746.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8198..8503
                     /gene="rpsN"
                     /locus_tag="ACHY3M_002865"
     CDS             8198..8503
                     /gene="rpsN"
                     /locus_tag="ACHY3M_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4317747.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8537..8929
                     /gene="rpsH"
                     /locus_tag="ACHY3M_002866"
     CDS             8537..8929
                     /gene="rpsH"
                     /locus_tag="ACHY3M_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4317748.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8942..9475
                     /gene="rplF"
                     /locus_tag="ACHY3M_002867"
     CDS             8942..9475
                     /gene="rplF"
                     /locus_tag="ACHY3M_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4317749.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9485..9838
                     /gene="rplR"
                     /locus_tag="ACHY3M_002868"
     CDS             9485..9838
                     /gene="rplR"
                     /locus_tag="ACHY3M_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4317750.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9853..10356
                     /gene="rpsE"
                     /locus_tag="ACHY3M_002869"
     CDS             9853..10356
                     /gene="rpsE"
                     /locus_tag="ACHY3M_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4317751.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10360..10539
                     /gene="rpmD"
                     /locus_tag="ACHY3M_002870"
     CDS             10360..10539
                     /gene="rpmD"
                     /locus_tag="ACHY3M_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4317752.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10543..10977
                     /gene="rplO"
                     /locus_tag="ACHY3M_002871"
     CDS             10543..10977
                     /gene="rplO"
                     /locus_tag="ACHY3M_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4317753.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10985..12316
                     /gene="secY"
                     /locus_tag="ACHY3M_002872"
     CDS             10985..12316
                     /gene="secY"
                     /locus_tag="ACHY3M_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4317754.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12348..12464
                     /gene="rpmJ"
                     /locus_tag="ACHY3M_002873"
     CDS             12348..12464
                     /gene="rpmJ"
                     /locus_tag="ACHY3M_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4317755.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12611..12967
                     /gene="rpsM"
                     /locus_tag="ACHY3M_002874"
     CDS             12611..12967
                     /gene="rpsM"
                     /locus_tag="ACHY3M_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4317756.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12984..13373
                     /gene="rpsK"
                     /locus_tag="ACHY3M_002875"
     CDS             12984..13373
                     /gene="rpsK"
                     /locus_tag="ACHY3M_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4317757.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13407..14027
                     /gene="rpsD"
                     /locus_tag="ACHY3M_002876"
     CDS             13407..14027
                     /gene="rpsD"
                     /locus_tag="ACHY3M_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4317758.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14053..15042
                     /gene="rpoA"
                     /locus_tag="ACHY3M_002877"
     CDS             14053..15042
                     /gene="rpoA"
                     /locus_tag="ACHY3M_002877"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4317759.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15083..15466
                     /gene="rplQ"
                     /locus_tag="ACHY3M_002878"
     CDS             15083..15466
                     /gene="rplQ"
                     /locus_tag="ACHY3M_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4317760.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15573..15941
                     /locus_tag="ACHY3M_002879"
     CDS             15573..15941
                     /locus_tag="ACHY3M_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFS4317761.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15952..16377
                     /gene="zntR"
                     /locus_tag="ACHY3M_002880"
     CDS             15952..16377
                     /gene="zntR"
                     /locus_tag="ACHY3M_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFS4317762.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16433..16651
                     /gene="arfA"
                     /locus_tag="ACHY3M_002881"
     CDS             16433..16651
                     /gene="arfA"
                     /locus_tag="ACHY3M_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFS4317763.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16648..17058)
                     /gene="mscL"
                     /locus_tag="ACHY3M_002882"
     CDS             complement(16648..17058)
                     /gene="mscL"
                     /locus_tag="ACHY3M_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4317764.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17188..18564)
                     /gene="trkA"
                     /locus_tag="ACHY3M_002883"
     CDS             complement(17188..18564)
                     /gene="trkA"
                     /locus_tag="ACHY3M_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFS4317765.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRALQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18586..19875)
                     /gene="rsmB"
                     /locus_tag="ACHY3M_002884"
     CDS             complement(18586..19875)
                     /gene="rsmB"
                     /locus_tag="ACHY3M_002884"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4317766.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(19921..20868)
                     /gene="fmt"
                     /locus_tag="ACHY3M_002885"
     CDS             complement(19921..20868)
                     /gene="fmt"
                     /locus_tag="ACHY3M_002885"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4317767.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20883..21392)
                     /gene="def"
                     /locus_tag="ACHY3M_002886"
     CDS             complement(20883..21392)
                     /gene="def"
                     /locus_tag="ACHY3M_002886"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4317768.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21522..22630
                     /gene="dprA"
                     /locus_tag="ACHY3M_002887"
                     /pseudo
     CDS             21522..22630
                     /gene="dprA"
                     /locus_tag="ACHY3M_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            22602..23075
                     /gene="smg"
                     /locus_tag="ACHY3M_002888"
     CDS             22602..23075
                     /gene="smg"
                     /locus_tag="ACHY3M_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFS4317769.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23104..23646
                     /locus_tag="ACHY3M_002889"
     CDS             23104..23646
                     /locus_tag="ACHY3M_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFS4317770.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23651..24223
                     /gene="tsaC"
                     /locus_tag="ACHY3M_002890"
     CDS             23651..24223
                     /gene="tsaC"
                     /locus_tag="ACHY3M_002890"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFS4317771.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24228..25046
                     /gene="aroE"
                     /locus_tag="ACHY3M_002891"
     CDS             24228..25046
                     /gene="aroE"
                     /locus_tag="ACHY3M_002891"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4317772.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25043..25300
                     /locus_tag="ACHY3M_002892"
     CDS             25043..25300
                     /locus_tag="ACHY3M_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFS4317773.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25276..25830)
                     /locus_tag="ACHY3M_002893"
     CDS             complement(25276..25830)
                     /locus_tag="ACHY3M_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4317774.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6721 a         6212 c         6782 g         6440 t
ORIGIN      
        1 ccgtaccgtt ggcgcgggcg ttgttgctaa agttctgggc taattacacg ttaattagtt
       61 ttgaattgaa aagggcgctt cggcgccctt tttgctgccc gtaatttgag ctcgaaatat
      121 ttagtcgtaa ttttgctcgc tgaactatgg cgcaaataaa actgtgctat tgtaatcata
      181 accattctca tttacacttt gcgcagaatt tgaacgggag caatcatgta cgtttgtttg
      241 tgtaatggtg taagtgacaa aaaaattcgc caggcagtcc gtcagtttca tccgcaatct
      301 ttccaacaat tgcgtaagtt tattcctgtg ggaaatcaat gcggtaagtg cattcgcgcc
      361 gcgcgcgaag tgatgcagga cgagttgatg cagatgccag aatttaaaga gattgcctga
      421 agaccacgct tattttttga catcccgcta gccccatcta cgctctaata gtggaagcgg
      481 agggactata aaatgaaagg tgatgttaaa atcataaatt atctcaataa actattggga
      541 aatgagcttg tcgcaatcaa tcagtatttt ctccatgccc ggatgtttaa aaactggggc
      601 ctgatgcgtc tcaatgacgt cgaataccat gaatccatcg atgagatgaa acacgctgat
      661 aaatatatcg agcgtatttt gtttttagaa ggaatcccca acttacagga tttaggcaaa
      721 ctggggattg gtgaagatgt cgaggagatg ctgcaatccg acctgcgatt agaactggaa
      781 ggcgcgaagg atctccgtga ggctatcgcg tatgccgaca gcgttcatga ctatgttagc
      841 cgtgatatga tgatcgagat ccttgccgat gaagaaggtc atattgactg gctcgaaact
      901 gagctggatc tcatcggtaa aatcggttta caaaattacc tgcaatcaca aataaaagtt
      961 aaagattaac ggcctgctag caaactatcc caacctacaa cgaaacccgc agccgccaga
     1021 aatggcccaa acggcagcgg gttttttaat gactcttttc ctctcatgag cagaccaaca
     1081 actacggctc cgcaagcgaa tgaggcagca aggaaaacca gtcgtggcaa gaaggtccag
     1141 gtatgccagg caccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta
     1201 tgacgcagta tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aataatcgcc
     1261 ccccataacg catcggctaa accgtcagga ttacaaacct gagagaacaa aagacctgac
     1321 cagagtaacg ggcaggtaaa tctgtcaggt aacaagccat gctttgcatc ccataaaaaa
     1381 agtaggacag ataaacaagc gtacaaaatc agaaagggaa ggttagcgtc cataaaacat
     1441 cctctgtaat tggagtatca ccatcgaata actccggatg ctcagcaaca gagaaatcgc
     1501 tgatttgtta acctggttcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg
     1561 aatacagctc gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca
     1621 gatttgcgta taatgcgcgg gcttgtcgta gttgacaaca ggttcaatct gaacctcagt
     1681 tagccgaatt tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca
     1741 ctctcccatc aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga
     1801 gctctggtct catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc
     1861 tgatcgatca agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc
     1921 gtggtccgat cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg
     1981 tcaacaaaga cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg
     2041 ttgagccaac cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag
     2101 acgtgcagat cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat
     2161 tggtttagtc ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat
     2221 cccagtaacc gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa
     2281 cgatggctac cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa
     2341 gcctgaagct ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt
     2401 ccgcctggct gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc
     2461 tgacgttaaa aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt
     2521 taagcgctgg aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt
     2581 tccgggttct atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc
     2641 aggtcagatg ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc
     2701 tgagcgcaac ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat
     2761 cgttaaacca gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc
     2821 agagcgcgct gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc
     2881 accaggttgt tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc
     2941 gtgctgaagt aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc
     3001 gttctggttc tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc
     3061 cgcaggacca cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc
     3121 tgtccgaact ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc
     3181 cgaaaactaa gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca
     3241 tcaccggtga gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg
     3301 tacgcgatgc aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga
     3361 ctgctgatgc tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct
     3421 gaaggtgctg cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa
     3481 caccatcgta ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca
     3541 gaaactgttt gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa
     3601 acgtcacgga cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa
     3661 agaaggccag aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg
     3721 gcagttgtta aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac
     3781 cctgagctgc acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt
     3841 ggtcgtaaca acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct
     3901 taccgtattg ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt
     3961 gagtacgatc cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc
     4021 cgttacatcc tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat
     4081 gctgcaatca aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt
     4141 cataacgtag aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac
     4201 gttcagatcg ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg
     4261 cgtaaagtag aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg
     4321 ctgcgcgttc tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc
     4381 ggtaccgcga tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt
     4441 aagcacccgg taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag
     4501 cgtactgata aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc
     4561 acgttctctc aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc
     4621 ggtggaaagc ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc
     4681 taacatgatc ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt
     4741 aaccgacgaa atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg
     4801 ccacgctgct gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc
     4861 gctaaacatc gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt
     4921 cgcggtaaga aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct
     4981 gtactggtca agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct
     5041 gacattgacg atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc
     5101 attatgccgc gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact
     5161 gtggttgtgt ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta
     5221 atggtattcg cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag
     5281 aattcgctga caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg
     5341 ctaaagcgtc cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca
     5401 ttcacactgc tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc
     5461 gtaaggtcgt agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta
     5521 agcctgaact ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg
     5581 ttatgttccg tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag
     5641 gtattaaagt tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt
     5701 accgcgaagg tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg
     5761 aagcgcacac cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc
     5821 tgggtggtat ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc
     5881 agcgtaaagg ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc
     5941 gtaaaatgca caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca
     6001 gcttcggtct gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac
     6061 gtcgtgctat gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg
     6121 acaaaccgat cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg
     6181 agtattgggt tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg
     6241 aagagctggc ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct
     6301 ttgtaactaa gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga
     6361 gctgaacacc gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc
     6421 aagtggccag ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt
     6481 taagacttta ctgaacgaga aggcgggtgc gtaatgaccg ataaaatccg tactctgcaa
     6541 ggtcgcgttg ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg
     6601 aaacacccga tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag
     6661 aacaacgaat gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag
     6721 actaaatcct ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc
     6781 tctcgatacg aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga
     6841 agcggtgtta taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc
     6901 tcagtagtag ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg
     6961 tcgccgacaa ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc
     7021 gtcgctacgc aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg
     7081 gtaaggtcaa aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc
     7141 gtcgcccgga cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca
     7201 acagcgagca gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg
     7261 agaagttcat gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca
     7321 gcgaaaatcc gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc
     7381 ggtaaagtta agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt
     7441 aagaaacatc agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa
     7501 gccgctattc aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt
     7561 gtaggcttta gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact
     7621 atcaagtaat ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag
     7681 ttaaaaaact catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga
     7741 agatcaccct gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg
     7801 cagcagcaga cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat
     7861 ctgttgcagg cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg
     7921 gcgaacgcat gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg
     7981 acttccgtgg cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc
     8041 gtgagcagat catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg
     8101 atattaccat taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct
     8161 ttgacttccc gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc
     8221 acgcgaagta aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa
     8281 agcgatcatc tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct
     8341 gcagactctg ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg
     8401 tcgtccgcat ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc
     8461 tatgcgcggt gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc
     8521 acgggagtaa agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg
     8581 taacggtcag gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc
     8641 aatcgccaac gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac
     8701 caagcctgaa ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat
     8761 tcagcgtgtc agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt
     8821 tatggcgggt ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc
     8881 agcgcgccag gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa
     8941 aatgtctcgt gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa
     9001 cggtcaggtt attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc
     9061 tgttgaagtt aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga
     9121 cggttgggca caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac
     9181 cgaaggcttc actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg
     9241 caatgtgatt aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg
     9301 tatcactgct gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt
     9361 gatcggccag gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa
     9421 gggtgttcgt tacgccgacg aagtcgtgcg taccaaagag gctaagaaga agtaaggtaa
     9481 cactatggat aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca
     9541 ggagctgggc gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt
     9601 aattgcaccg aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc
     9661 tgaacaactg aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc
     9721 tgaacgcgct ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata
     9781 tcatggtcgt gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg
     9841 tagaggtgta agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc
     9901 gcggtaaacc gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg
     9961 actgtagttg gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt
    10021 ccagcagcga tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg
    10081 aataacggca ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg
    10141 cagccggctt ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa
    10201 gtcgctgggg ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg
    10261 gttcgtgcaa ctattgatgg cctggaaaat atgaattctc cagaaatggt cgctgccaag
    10321 cgtggtaaat ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt
    10381 actcaaaccc gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg
    10441 ggtctgcgtc gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg
    10501 atcaacgcgg tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg
    10561 tctccggccg aaggctccaa aaaggcgggt aaacgcctgg gtcgtggtat cggttctggc
    10621 ctcggtaaaa ccggtggtcg tggtcacaaa ggtcagaaat ctcgttctgg cggtggcgta
    10681 cgtcgcggtt tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc
    10741 acttctcgta aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa
    10801 ggcggtgttg tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag
    10861 ttcgcgaaag tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt
    10921 gttactaaag gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta
    10981 gcagatggct aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct
    11041 gaaacgcaga ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat
    11101 tccgatccct ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac
    11161 catcattgag atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc
    11221 tctggggatc atgccgtata tttcggcgtc gatcattatc cagctgctga cggtagttca
    11281 cccaacgttg gcagaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta
    11341 cacccgttac ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct
    11401 gccgaatatg cctggtatgc aaggcctggt gattaaccca ggctttgcat tctacttcac
    11461 cgctgttgta agtctggtca caggaacgat gttcctgatg tggttgggcg aacagattac
    11521 tgaacgaggt atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact
    11581 cccgccagcc attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt
    11641 gttgctgttg gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg
    11701 tggtcaacgc cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc
    11761 tgcacagagc acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt
    11821 cgcttccagt attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg
    11881 ttggaactgg ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt
    11941 actctatgcg tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc
    12001 gcgtgaaaca gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg
    12061 agagcaaacg gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct
    12121 gtatattacc tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt
    12181 ctacttcggt gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca
    12241 agtgcaaact ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa
    12301 aggctacggc cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg
    12361 cttccgtcaa gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg
    12421 tgatttgcag tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt
    12481 tcttgcaaag ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg
    12541 tttccatttg agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta
    12601 ggagtgcata gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat
    12661 cgcattaact tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc
    12721 gggtatcgct gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg
    12781 tgacgaagtt gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat
    12841 caagcgcctg atggatcttg gttgctatcg cggtttgcgt catcgtcgtg gtctcccggt
    12901 tcgcggtcag cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa
    12961 gaaataatcg gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa
    13021 gaaaacaagt ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga
    13081 ctatcactga tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc
    13141 gtggttctcg caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg
    13201 ccgtgaaaga atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc
    13261 gcgaatctac tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg
    13321 tgactccgat ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg
    13381 ttttccaggt ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga
    13441 gccgtcgtga gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt
    13501 gtaaaattga acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg
    13561 gtgtgcagtt gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt
    13621 tccgtaacta ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg
    13681 ctctgctgga aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg
    13741 cagaagcacg tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaaca
    13801 tcgcttctta tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc
    13861 agtctcgcgt gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acctggctgg
    13921 aagttgatgc tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt
    13981 ctgcggacat taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa
    14041 agagaggaca caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc
    14101 gagcaagtga gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc
    14161 catactctgg gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg
    14221 accgaggttg agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa
    14281 gatatcctgg aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat
    14341 gaagttattc ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc
    14401 cacgacggtg atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag
    14461 aacgcgtcta ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct
    14521 acccgaattc attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc
    14581 tacagccctg tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc
    14641 gacctggaca agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg
    14701 attcgtcgtg cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat
    14761 gtacgtcagc ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct
    14821 gttgacgatc tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac
    14881 tatatcggtg atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt
    14941 aaaaaatctc ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg
    15001 cgcctggaaa actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg
    15061 ttttactgag aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa
    15121 cagcagccat cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat
    15181 catcaagacg actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac
    15241 tcttgccaag actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa
    15301 cgagatcgtg gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg
    15361 ttacactcgt attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat
    15421 cgagctggtt gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta
    15481 aaaaaacccg ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc
    15541 tgtactcttt ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc
    15601 gtcatatagc agaagcgcaa gcgaaaggtg agtttgataa cctggcaggt agcggcgaac
    15661 cattgatact ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc
    15721 tgaagaatgc cggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc
    15781 tggatatcct gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat
    15841 tgtcattact ggaattgaag ctgcgacaag ctggattgag taccgatttt ttacgcggcg
    15901 attatgctga caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc
    15961 attggtgagc tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa
    16021 cagcagatga tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc
    16081 gatctccagc gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg
    16141 atccgcgagt tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa
    16201 ggcattgtgc aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg
    16261 cagcgttcct tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat
    16321 tgttcgattc ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt
    16381 tttgcactga caggattaca ctcgcgccgt taaataacca actggagttt ttatgagccg
    16441 atatcagcat actaaagggc agataaagga taatgcgata gaagcattac tacatgatcc
    16501 cttattccga cagcgagtag agaaaaataa gaaagggaaa ggcagttaca tgcgaaaagg
    16561 aaaacatggc aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac
    16621 cactggtctt ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc
    16681 acgaatttct gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc
    16741 ttttttccga ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat
    16801 cagaaaatca aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc
    16861 cccctgcgca tcgcgtagcg tgacagcaaa ctgtttaaaa tcgatcccgc caattaataa
    16921 gcccagagga ggcatgatga tatcggcaac cagtgaagag acaatcttcc cgaatgccgc
    16981 accgataatg acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa
    17041 ttctttaata atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat
    17101 gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata
    17161 ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggtt ggaagagtct
    17221 ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc
    17281 aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat
    17341 cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc
    17401 atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc
    17461 aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga
    17521 aatcgcaata tcgatcacac tcccctgaac cagatccaca taagcgcgac gctggatcaa
    17581 taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc
    17641 gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag
    17701 ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc
    17761 ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg
    17821 cgccagccct gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc
    17881 cagtcgctgt aattcactca tcaccgcacg gatatgctgt gaagcggcaa taaagaacac
    17941 ttcatcacca gcttccacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa
    18001 aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcgtt
    18061 accaatcagc gggccgccat aataggcttt aaccacagcg aggctgactt taccctcagc
    18121 gaagttcacc acctgcaatg cgccgggata ctcaatcagt cggtaaatat tatcgataac
    18181 caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc
    18241 ggcatcgcgc acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag
    18301 tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag
    18361 catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg
    18421 cacgactcgc aggtcaaatt tatcctgcaa ggcccgcaga cgctcaccgt tggtatcgac
    18481 aacagtgata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc
    18541 ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact ttttgattag
    18601 cttagcgtaa aagaagccgt cgccctcttc ggtaccaggc agattttgtt tacccggttg
    18661 ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aggctttaat
    18721 ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga
    18781 ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat
    18841 atcgcgatcg cgacgtaacc atttaatatc cggatggcga cgaatcacac cggttgctga
    18901 acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta
    18961 gcggccatca ccttgtttca cggtcgcttt cataccaagg cgttttaaat tgtcgtaaac
    19021 gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc
    19081 aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt
    19141 ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc
    19201 gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta cagcatcagg
    19261 gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc aacaatgcaa gccagctgtc
    19321 gcgggaatga tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattagcttc
    19381 gacgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg
    19441 atgcagataa cgtgcatcac tggcataaaa cccggctaat aactcttctt gctgacgctg
    19501 gaactggcgt aatacaccgt taatcagccc cttaagttgc gggcgcttaa ttgcgacagc
    19561 gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata
    19621 cacaccaacc ataatcaggt aatgcacggt ccgctgtttg ccggtcatcg gacgggccat
    19681 taacttatta atcagccagt ctaactgtga aagcgtacgc agtacgccaa agcacaactc
    19741 ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca gaatgttgct
    19801 taatgattgc ccttgctcga cgatttgttc aacggcctgt gccgccatgc tacgtaaatt
    19861 acgttgtttt ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta
    19921 tcagaccaga cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc
    19981 gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc catcaccagt
    20041 cgcaacctga atgccttgtt tattggcttc aaggatcgtt cctggtgcag cgttggttgc
    20101 cgtatcaatg accgacgctt tccagacttt aaccggttgt ccttcaattt ccagccagct
    20161 cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgcgctgccg aaagcgacca
    20221 gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag tttcgtcctg
    20281 aacttctggt ttcgccgtgc cgtctgctag ttgtttcaac gtggtgataa gcccttgtgg
    20341 gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca
    20401 ggagagctta taaagcatgt caccggtgtc taaaccaaca tccatttgca taatggtcac
    20461 accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg
    20521 tggcagcagt gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt
    20581 aggcagaatt aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag
    20641 ttgctggctt tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc
    20701 agccagaact ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg
    20761 ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc
    20821 tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat
    20881 ccttaagccc gggctttcag acgatctagt ttttcaactt tctgacgaat acgttgttgt
    20941 ttcagcggtg acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga
    21001 atacagatgg ctaacagccc ctctgcttcc agttcaaatg gtttaccgtc gcggtcaagg
    21061 gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg gatcgacagg
    21121 caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc
    21181 agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc
    21241 tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg
    21301 atacgctgta tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg
    21361 tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta
    21421 gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa
    21481 tcgcaaagat tgctaaggct gcttatggca gggagataag gatggtcgat acagaaattt
    21541 ggctgcgttt aatgagtatc agcagcttgt acggcgatga tatggtccgt atagctcact
    21601 ggctggcaaa acagtcgcaa attgatggat tgcagcaaac agggcttaca ttgcggcagg
    21661 cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc
    21721 aacccaacca tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttctggcga
    21781 cgacagatta ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc
    21841 agcttgccgt agtggggagt cgggcgcatt catggtatgg cgagcgatgg ggacggttat
    21901 tttgcgaaac tctggcgacg cgtggagtga caattacgag tggactggcg cgtggaatcg
    21961 atggtgtggc gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga
    22021 atggacttaa taccattcat ccccgccgcc atgcccgact ggctgccagt ctgcttgaac
    22081 atggcggagc tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc
    22141 cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggtgg
    22201 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
    22261 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
    22321 tgtgacggaa ccggaagata ttctggaaaa cttgcaattt ggattgcact ggttgccaga
    22381 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
    22441 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
    22501 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
    22561 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
    22621 atttgtttga aacctatatt cacacagaag ctgagttgcg tgtggatcaa gacaaacttg
    22681 aacaggatct taccgacgca ggatttgatc gagaagatat ctacaatgcc ctgctttggc
    22741 tggaaaagct tgcggattat caggaaggac tggcagaacc gatgcaactg gcctctgatc
    22801 cactctccat gcgtatttat acccctgagg agtgtgaaag actggatgcc agctgtcgtg
    22861 gttttctgct tttccttgag caaattcagg tgcttaacct tgaaacccgt gaaatggtga
    22921 tagagcgagt gctcgcgctg gataccgcag agttcgacct ggaagacctg aaatgggtaa
    22981 tcctgatggt gttgttcaat attccgggct gcgaaaatgc gtaccagcaa atggaagaat
    23041 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
    23101 gttatggcga aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
    23161 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
    23221 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
    23281 gaggggcagg tttgccttgc atgtggcgca aatctggtat tacgccaggg acgctttggt
    23341 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
    23401 gacgaaacag caattacatg cccccaatgc cggacgggcc atctggtcca gcgccgctcc
    23461 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
    23521 ttcaaaccca tagctggaga atgccctgag tgtcattatc cactactcat cgaaaagaaa
    23581 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
    23641 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
    23701 caatgaagaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgttg ggtgcgatcc
    23761 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
    23821 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgct
    23881 gactgatgcg cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca cctttgtctt
    23941 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
    24001 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
    24061 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
    24121 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
    24181 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
    24241 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
    24301 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
    24361 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
    24421 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgctg
    24481 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtatag
    24541 gtttgttaag cgatctggaa cgtctgtctt ttatccgtcc tggtttacgt attctgctca
    24601 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
    24661 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
    24721 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
    24781 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
    24841 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
    24901 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
    24961 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
    25021 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gagaagagtg
    25081 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggcatgc aactgacatg
    25141 tgcgatctct ggcgagagtc tggcgtatcg ttttactgga gatacgccag aacagtggtt
    25201 agcgagtttt cgtcagcatc gctgggatct ggaagaagaa gcggaaaact taattcagga
    25261 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
    25321 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
    25381 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
    25441 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
    25501 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
    25561 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
    25621 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
    25681 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
    25741 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
    25801 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
    25861 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
    25921 gtgtgcgaat gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga tcaaaaaagt
    25981 acttgtgcaa aaaattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
    26041 cttcacaaga tggtcgtaac aacgaagaga aaaaatcctg aaattaaggg ttgactctga
    26101 aagaggaaag cgtaatatac gccacctcgc gacagtgagc tgaaagccgc gtcgc
//
LOCUS       DAZUFB010000061        25394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZUFB010000061 DAZUFB010000000
VERSION     DAZUFB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.061"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHY3M_002894"
     CDS             complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHY3M_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4317775.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHY3M_002895"
     CDS             complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHY3M_002895"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFS4317776.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHY3M_002896"
     CDS             complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHY3M_002896"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFS4317777.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4774..5511
                     /gene="bamD"
                     /locus_tag="ACHY3M_002897"
     CDS             4774..5511
                     /gene="bamD"
                     /locus_tag="ACHY3M_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFS4317778.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5782..6123
                     /gene="raiA"
                     /locus_tag="ACHY3M_002898"
     CDS             5782..6123
                     /gene="raiA"
                     /locus_tag="ACHY3M_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4317779.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6226..6351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6227..6274
                     /gene="pheL"
                     /locus_tag="ACHY3M_002899"
     CDS             6227..6274
                     /gene="pheL"
                     /locus_tag="ACHY3M_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFS4317780.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6373..7533
                     /gene="pheA"
                     /locus_tag="ACHY3M_002900"
     CDS             6373..7533
                     /gene="pheA"
                     /locus_tag="ACHY3M_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFS4317781.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHY3M_002901"
     CDS             complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHY3M_002901"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFS4317782.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQMRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHY3M_002902"
     CDS             complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHY3M_002902"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFS4317783.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHY3M_002903"
     CDS             9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHY3M_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFS4317784.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10503..11021
                     /gene="yfiR"
                     /locus_tag="ACHY3M_002904"
     CDS             10503..11021
                     /gene="yfiR"
                     /locus_tag="ACHY3M_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFS4317785.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11011..12237
                     /gene="dgcN"
                     /locus_tag="ACHY3M_002905"
     CDS             11011..12237
                     /gene="dgcN"
                     /locus_tag="ACHY3M_002905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFS4317786.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12253..12735
                     /gene="yfiB"
                     /locus_tag="ACHY3M_002906"
     CDS             12253..12735
                     /gene="yfiB"
                     /locus_tag="ACHY3M_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS4317787.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHAHMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12812..13159)
                     /gene="rplS"
                     /locus_tag="ACHY3M_002907"
     CDS             complement(12812..13159)
                     /gene="rplS"
                     /locus_tag="ACHY3M_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4317788.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13201..13968)
                     /gene="trmD"
                     /locus_tag="ACHY3M_002908"
     CDS             complement(13201..13968)
                     /gene="trmD"
                     /locus_tag="ACHY3M_002908"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4317789.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPLVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13999..14547)
                     /gene="rimM"
                     /locus_tag="ACHY3M_002909"
     CDS             complement(13999..14547)
                     /gene="rimM"
                     /locus_tag="ACHY3M_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4317790.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14566..14814)
                     /gene="rpsP"
                     /locus_tag="ACHY3M_002910"
     CDS             complement(14566..14814)
                     /gene="rpsP"
                     /locus_tag="ACHY3M_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4317791.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15063..16424)
                     /gene="ffh"
                     /locus_tag="ACHY3M_002911"
     CDS             complement(15063..16424)
                     /gene="ffh"
                     /locus_tag="ACHY3M_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4317792.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16591..17382
                     /gene="ypjD"
                     /locus_tag="ACHY3M_002912"
     CDS             16591..17382
                     /gene="ypjD"
                     /locus_tag="ACHY3M_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFS4317793.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17403..18689
                     /gene="yfjD"
                     /locus_tag="ACHY3M_002913"
     CDS             17403..18689
                     /gene="yfjD"
                     /locus_tag="ACHY3M_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFS4317794.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18744..19337)
                     /gene="grpE"
                     /locus_tag="ACHY3M_002914"
     CDS             complement(18744..19337)
                     /gene="grpE"
                     /locus_tag="ACHY3M_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4317795.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19460..20338
                     /gene="nadK"
                     /locus_tag="ACHY3M_002915"
     CDS             19460..20338
                     /gene="nadK"
                     /locus_tag="ACHY3M_002915"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFS4317796.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20424..22085
                     /gene="recN"
                     /locus_tag="ACHY3M_002916"
     CDS             20424..22085
                     /gene="recN"
                     /locus_tag="ACHY3M_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4317797.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22234..22575
                     /gene="bamE"
                     /locus_tag="ACHY3M_002917"
     CDS             22234..22575
                     /gene="bamE"
                     /locus_tag="ACHY3M_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFS4317798.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22637..22927)
                     /gene="yfjF"
                     /locus_tag="ACHY3M_002918"
     CDS             complement(22637..22927)
                     /gene="yfjF"
                     /locus_tag="ACHY3M_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFS4317799.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22917..23354)
                     /gene="ratA"
                     /locus_tag="ACHY3M_002919"
     CDS             complement(22917..23354)
                     /gene="ratA"
                     /locus_tag="ACHY3M_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFS4317800.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23525..24007
                     /gene="smpB"
                     /locus_tag="ACHY3M_002920"
     CDS             23525..24007
                     /gene="smpB"
                     /locus_tag="ACHY3M_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4317801.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24222..24584
                     /gene="ssrA"
                     /locus_tag="ACHY3M_002921"
     tmRNA           24222..24584
                     /gene="ssrA"
                     /locus_tag="ACHY3M_002921"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24856..25104
                     /locus_tag="ACHY3M_002922"
     CDS             24856..25104
                     /locus_tag="ACHY3M_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4317802.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(25115..25186)
                     /locus_tag="ACHY3M_002923"
                     /pseudo
     CDS             complement(25115..25186)
                     /locus_tag="ACHY3M_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            25261..>25394
                     /locus_tag="ACHY3M_002924"
     CDS             25261..>25394
                     /locus_tag="ACHY3M_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317803.1"
                     /translation="MTRSAYCASFCVSFYWTQANDAGDTIPARPTYNSKIRSSAFTYN
                     T"
BASE COUNT         6486 a         6693 c         5959 g         6256 t
ORIGIN      
        1 tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagcgaaaaa
      121 tataaaaact ttatcgttcg ctcatttttc aggcaaagca tcttaaatat agtcttttcc
      181 gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattta ctggacggcg
      241 acaatccggt cttcattaac ttccaggcga atcactttac ccggaaccaa ttcaccagac
      301 agtatttgct gtgccagcgg gttttcgatc tgctgctgga tagcacgttt caaaggacgt
      361 gcaccataga ctggatcgta accgttctcg ctcagcagtt tcagcgcctc gtcagaaatg
      421 tggatttcat aaccacgttc ttccagacgt ttgtacagac gtttcaactg aatctgcgca
      481 atcgaggcaa tgtgctgttc acccagcgga tggaagacca ccacttcatc aatacggtta
      541 atgaattccg gacggaagtt atggcttacc acaccgagca ccagctcttt catgtgcgca
      601 taatccagtt caccgaagcg ttcctgaatc aaatcggaac cgaggttaga ggtcataatg
      661 acaaccgtat tacggaagtc gaccgttctc ccctgcccgt cagtcagacg accatcatcc
      721 agtacctgca acagaatgtt gaagacatcc ggatgcgctt tttccacttc atccagcagg
      781 atgacggaat acggacgacg acgcacagct tcggtcaggt agccaccttc ttcataaccg
      841 acatatcccg gaggcgcacc aaccagacga gacaccgagt gtttctccat aaactcggac
      901 atatcgatac ggaccatcgc ctcgtcgcta tcaaacataa agttcgccag cgccttacaa
      961 agctcagttt tccccacacc cgttgggccg aggaacagga atgaaccaat cgggcgattt
     1021 ggatccgcca accccgcacg gctacggcga atagcgttag ataccgcatc aaccgcttcg
     1081 ttctgaccaa tgacgcgatg gtgcagttct tgctccatac gcagcagttt ttcgcgctcg
     1141 ctttccatca tgcgagaaac cggaatcccc gtccaacgcg ccagcacttc agcaatttcg
     1201 gcgtcggtca ctttattacg caacagacgc atagttttgc cttcgagctg cgttgcggct
     1261 tccagttgct tttccagttc cgggattttg ccgtattgca gttcagacat ccgcgccagg
     1321 tcccccacac ggcgagcctg ttcaatagcg attttcgcct gttccagttc cgctttaatg
     1381 gtctgcgtac cagaaagcga tgccttctct gctttccact cttcttctaa ctcggagtac
     1441 tgacgttctt tgtcgctcag ttcttcgttg agcatatcca ggcgtttttt actggcttca
     1501 tcagactctt tcattaacgc ctgttgttcc agtttgagct ggatgatacg acgatcgagt
     1561 cggtcgagtt cttctggttt tgagtcaatc tgcatacgaa tgctggatgc tgcttcatcg
     1621 atcaggtcga tggctttatc cggcagctga cggtcagcaa tgtagcgatg agacaacgtc
     1681 gccgctgcaa caattgccgg gtcagtaatt tgcacatggt ggtgcaattc gtaacgttct
     1741 ttcaggccac gcagaatcgc aatggtatct tcaacagaag gctcggcaac aaacactttc
     1801 tggaaacgac gttccagcgc agcatctttt tcgatgtact ggcgatattc gtcaagcgtc
     1861 gtggcaccta cgcagtgcaa ttcaccacgc gccagcgccg gtttcagcat gtttccggcg
     1921 tccattgcgc catcggcttt acccgcgccg accatggtat gtaattcgtc gataaacagg
     1981 atgacgttgc cttcctgttt ggcaagatcg ttaagcacgc cttttaaacg ttcttcaaac
     2041 tcaccgcgat atttcgcccc agccaccagc gcgcccatat ccagcgccag tacgcggcgg
     2101 cctttcaatc cttccggcac ttcgccgttg ataatacgct gcgccagacc ttcaacgatg
     2161 gcagttttac cgacgccggg ttcaccaatc agtaccgggt tatttttagt acgacgttgc
     2221 agcacctgaa tggtacggcg aatttcttca tcacgaccaa tcaccggatc gagtttgccc
     2281 tgttcggctc gttcggtaag gtcgatggta tattttttca aagcctgacg ttggtcttca
     2341 gcaccttgat cgttcacgct ttcacctcca cgcatttgtt caatcgcttg agtaatgttg
     2401 gcggtggtcg cccctgctgc tttcaggatg tcggccagcg tgccgcgaga ctcaagtgcc
     2461 gccagaacga acagttctga cgagataaag ttatcaccac gtttttgcgc cagcttgtcg
     2521 caaagattaa gaacgcgcac cagatcctgt gatggctgga catcaccacc agtaccttca
     2581 acctgcggta aacgatttaa tgcctgattg atatctgtgc gcaactggcc agcatttatg
     2641 ccagcggatg ttaataaagg actaaccgaa cccccttcct gattcagcag ggcgctcatt
     2701 aaatgaagtg gttcgataaa ttggttgtcg tgcccgagtg caagtgattg ggcatcggca
     2761 agagcaagct ggaatttatt agtaagacga tccagacgca taactcctcc cataacggat
     2821 caaagttgct actggagatt aaatgaggtc atccctcaat tattcaaggt tattgaccag
     2881 attaatgtga aaagaaaatc acgcgtaccg gatcgtcttg attctttagg ttatatcagc
     2941 caaatgaaac ttgccatacg accggtggtc ttgtcgcgac gataagagaa gaaagtctca
     3001 ttttccgtat atgtacaacg gtcgccgccg aaaatttgct caacacccac gttcgccaga
     3061 cgctgccggg caagctgata aatatccgcc agatacttat caccatgctg aatgaaagct
     3121 gcacttgctt tagcgtctac tgccataaac gcctcgcgaa cctccacccc cacttcgaac
     3181 gcgcgtggac caattgccgg ccctaaccag gcgagaatat tttccggatt atcagcaaaa
     3241 caggaaaccg tctcttccag cacgcctgcg cacagtccac gccagccagc atgagcggcg
     3301 gcgacttccg ttcccgctcg attgcaaaac agcacaggga ggcagtcggc agtcatcact
     3361 gcgcaaaccg tgccgggcgt attgctataa gaggcatctg ctcgttttga ggcataaggt
     3421 tcgccagtaa gcttaagcac atctttgccg tgtacctgct caagccagac cggtttagaa
     3481 ggcaaattgc ccgcagcaaa aagtcgcttg cgattctcct caacgtgatc cgggttatcg
     3541 ccacaatggg caccgaggtt gagcgagtca tacgggggca agctcacgcc gccgatacga
     3601 gaagagctac aggccgcaac accttttggt agcggccact gcgggacaat cagcttactc
     3661 ataaccagtc cacttcatcc ttatgctctt cgaaatcggc gcgcatcacc tcaatcagct
     3721 ccaccatatc ttgtggaata ggcgcatgcc attccatttc gatgccggag atcgggtgat
     3781 aaagacgcag catggttgca tgaagcgcct ggcggtcaaa cttacgcagc atggagataa
     3841 atgcttccga agcacctttt ggcggacgcg gacggccacc ataaaccgga tcgcccacca
     3901 gcggatgagt gatatgggcc atatgcacgc ggatctggtg cgtacgtcca gtttccagac
     3961 gcaaccgcag acgcgtgtgc acacggaagt gttccatgat gcgatagtga gtcaccgccg
     4021 gtttgcccat cggatgcacc gccatgtgag tacgtttggt cgggtggcgg ctgatgggtt
     4081 catctactgt accgccagcg gtcatatgac caatcgccac cgcttcatac tcacgagtaa
     4141 tttcacgccg ttgcaaagat tcgactaaac gcgtctgagc cggaacggtt tttgccacaa
     4201 ccatcaggcc agtggtgtct ttatccagac gatggacgat gcccgcacgt ggtacatcgg
     4261 caatgggcgg atagtaatga agcaacgcat tcagtaccgt gccatccggg ttacccgcgc
     4321 caggatgtac caccaggtcg cgcggtttat taatgacgat gatatcttca tcttcataga
     4381 cgatatccag cgggatatcc tgcggttcaa aacgcgcttc ttcttcaatc tcagcattga
     4441 tggcaacctg ctcgccaccc aatacttttt ctttcggctt atcacaaact ttgccgttta
     4501 ccagcacgcg ctggtcgagg atccattctt ttattcgcga acgtgaataa tccgggaaca
     4561 tttcggccaa agcctgatct aagcgttgac cgagttggtt ttcggacacc gttgcagtga
     4621 gctgtactcg ttgtgccata tatactgctt cttcgtttat cgttggggtt ttacggcttt
     4681 gccgtttaat atagtgtgct attgtagctg gtcttaaccg ggagcaggaa cagagaatct
     4741 cccgtattac attttgagga aagtcaaaac gtcatgacgc gcatgaaata tctggtggca
     4801 gccgccacac taagcctgtt tttggcgggt tgctcggggt caaaggaaga agtacctgat
     4861 aatccgccaa atgaaattta cgcgactgca caacaaaagc tgcaggacgg taactggaga
     4921 caggcaataa cgcaactgga agcgttagat aatcgctatc cgtttggtcc gtattcgcag
     4981 caggtgcagc tggatctcat ctacgcctac tataaaaacg ccgatttgcc gttagcgcag
     5041 gctgccatcg atcgttttat tcgccttaac ccgacccatc cgaatatcga ttatgtcatg
     5101 tacatgcgtg gcctgaccaa tatggcgctg gatgacagtg cgctgcaagg gttctttggc
     5161 gttgaccgta gcgatcgcga tcctcaacat gcacgagctg cgtttagtga cttttccaaa
     5221 ctggtgcgcg gctatccaaa cagtcagtac accaccgatg ccaccaaacg tctggtattc
     5281 ctgaaagatc gtctggcgaa atatgaatac tccgtggccg agtactatac agaacgtggc
     5341 gcatgggttg ccgtcgttaa ccgcgtagaa ggaatgttgc gcgactaccc ggatacccag
     5401 gctacgcgtg atgcgctgcc gctgatggaa aatgcatacc gtcagatgca gatgaatgcg
     5461 caagctgaaa aagtagcgaa aatcatcgcc gcaaacagca gcaatacata acagaaacct
     5521 gaaatacaaa acggcagccc ttgagctgcc gtttttttat tctgttagtt gtgaaactga
     5581 agcgatttag tcactatcga tctcatcaaa tatggctcgc tttgagatat tcctcaagta
     5641 aaaaaacacc tcttcctgcg atttctcaca aaaaagattc gttgacaaaa agtgacaaaa
     5701 ttatgagatt cccatcacac attttgacat caggagcggt atgctgaatt caccaagacg
     5761 ggaagacaag aggtaaaatt tatgacaatg aacattacca gcaaacaaat ggaaattact
     5821 ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat
     5881 ctgattaatc cacatatcat tctgtccaaa gagccacaag ggtttgttgc tgacgccaca
     5941 atcaatacac ctaacggcgt tctggttgcc agtggtaaac atgaagatat gtacactgca
     6001 attaacgaat tgatcaacaa gctggaacgg cagctcaata aactgcagca caaaggcgaa
     6061 gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag
     6121 tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt gttgacagcg
     6181 tgaaaacagt acgggtactg tactaaagtc acttaaggaa acaaacatga aacacacacc
     6241 gtttttcttc gcattctttt ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac
     6301 agaatgcgaa gacgaacaat aaggcctccc aaatcggggg gcctttttta ttgataacaa
     6361 aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg
     6421 ctggatgaaa aattattagc attactggca gagcggcgcg aattggccgt cgaggtagga
     6481 aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg cgatttactg
     6541 gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg
     6601 ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca gcaacatctc
     6661 aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc
     6721 catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc
     6781 gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta
     6841 ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct gcaacatacc
     6901 agcttgtcga ttgttggcga gatgacgtta actatcgacc attgtttgtt ggtctccggc
     6961 acgactgatt tatccactat taatacggtc tacagccatc cgcagccatt ccagcaatgc
     7021 agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag tacgtctgcg
     7081 gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct
     7141 ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca gcaacaaaac
     7201 ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca ggttccggcg
     7261 aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg
     7321 gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat
     7381 ccatgggaag agatgtttta tctggatatt caggccaatc ttgaatcagc ggaaatgcaa
     7441 aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg ctgttaccca
     7501 agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca
     7561 tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca cgcggctttc
     7621 actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat
     7681 aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta
     7741 acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat aaagctgcgg
     7801 atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag
     7861 cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat aagcaaaagt
     7921 agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga cagcgctaat
     7981 acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg
     8041 cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa
     8101 catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc
     8161 atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc
     8221 gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg catcggcaac
     8281 aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcatctgat aacccgatag
     8341 tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg
     8401 acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt aagattcacg
     8461 catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc
     8521 tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa
     8581 gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttcgcta ataaattcag
     8641 cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat
     8701 aaacctctta agccacgcga gccgtcagct gcccgttcag atcctgatga atttcacgca
     8761 gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca
     8821 tttcactgcg cggttgctca gaagactgat tgccctcgtg gatattactt tcgatcatca
     8881 gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag
     8941 gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc
     9001 gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg
     9061 gcgctttacc accgcgcagg atgacatgcc cgtccggatt cccctgagtt tgtagcaacg
     9121 caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag
     9181 cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa
     9241 gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc
     9301 agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg
     9361 gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta
     9421 catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac
     9481 ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa
     9541 gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac
     9601 cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttctacgcg
     9661 agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg
     9721 gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga
     9781 tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat
     9841 aacacataaa gtaaagtctt caatccatat ttcgtacatt tttatttaca caggcaattt
     9901 agtcgcgatt tcaaccctta cttctgtata gataaattta cactcccttt gaaaacaatc
     9961 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct
    10021 ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacctt gaccagcacc
    10081 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat
    10141 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa
    10201 aagaaaactt gccagaccga ttttacttat gcccggggac tttccggtaa aagctttcct
    10261 gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca aaaaggggct
    10321 gatgaaaatg ccagcacaat acgattaaat taattaaaac cccacaaata aattaaacat
    10381 aagattttac tcattgtgtt ttatttctca cattgatgac gatcgcatga aaaaatgata
    10441 ttttaatgag cattctgaaa aaatatcgat gcatttcgag cgaagatggt ggggatccct
    10501 gaatgcgttt ttctcaccga ctgttcttac ttctgattct cctgctgacg ggagcaccaa
    10561 tactcgctca ggagccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg
    10621 tcagttacac ccgctggcct gcgctatcag ggccaccaaa gttatgtatc ttttcatctt
    10681 cacgttttag taccgcccta caagagaatg ccgcgacatc tttgccttat ctgcctgtca
    10741 tcattcacac tcaacaagag gcgatgattt caggctgcaa tggtttttat tttggcaacg
    10801 agtcaccgac attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa
    10861 ttgccgaaca aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata
    10921 atgacgtcag atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc
    10981 cggatgtctt aatgctcgca cggaagaaaa atgatggata acgataattc tcttaataag
    11041 cgccccacgt ttaaaagagc attacgcaac atcagtatga ccagcatatt tatcactatg
    11101 atgctgatct ggttgctgct ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa
    11161 aaaaacctgg cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt
    11221 gccgatggcc ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg gcaattttca
    11281 actgcagaag tacgtgataa gcagcaaaat attctggcgt cctggcatta cacccgtaag
    11341 gatccaggcg atactttcag caatttcata agccactggc tcttccccgc ccccatcatt
    11401 cagccgattc gtcacaatgg tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt
    11461 tcaatcagcc attttatctg gttttcgctc gccgtactga ccggttgtat tctgctggca
    11521 tcaggcatcg caattaccct cacccgccat ttgcacaatg gcctggtaga agcgctgaaa
    11581 aatatcaccg atgtcgtaca tgatgtgcgt tccaaccgca atttttcccg acgagtttcg
    11641 gaagaacgta tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa
    11701 atggaagagt ggcagcttcg tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta
    11761 catgacccat taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caatacgttg
    11821 atgaacaatt ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc
    11881 aaatatatca atgatacctg gggtcatgcg acgggcgata gagtcttgat tgaaatcgca
    11941 aaacggttag ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa
    12001 ttcgctatgg tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca
    12061 ctgacacaaa tctttaatct cccgtttgat cttcataatg gccatcagac caccatgaca
    12121 ttaagcattg gttacgcgat gaccattgag cacgcctctg cggaaaaatt acaagagctt
    12181 gccgatcaca atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa
    12241 ggatatatcg atatgataaa gcacctggta gcacccctga ttttcacctc actaatactg
    12301 actggctgcc agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct
    12361 tatggattta ctgaatccgc cggcgactgg tcgctgggct tatcagatgc cattctgttc
    12421 gcaaaaaatg actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc
    12481 aaattggcct cgacagggct aacacatgcc catatggatg gacacaccga taactatggt
    12541 gaagacagtt acaacgaagg cttatcattg aaacgggcga atgtcgtggc cgatgcatgg
    12601 gctatgggtg gacaaattcc gcgcagcaat ctcaccacac agggtttagg aaaaaaatat
    12661 cccatagcca gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg
    12721 attactaccc cttaaaacac cagcaaacag ataaaaaaag ggccagccaa ttggccagcc
    12781 cttcttaaca ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag
    12841 ccttaccagt acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt
    12901 tgacagaaat gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc
    12961 cgttggaaat tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa
    13021 ccacgccctc gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt
    13081 ccacggtatc acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt
    13141 gcttaataat gttgctcata atttaatctc ttatcctggg taaactgata tctcgggggc
    13201 ttacgccatc ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag
    13261 caaccttgct tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca
    13321 ggtacggccc agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag
    13381 taacactagc ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc
    13441 cagcaatcct tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg
    13501 aataaaccgg gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac
    13561 gtaatcgccg attgaccatt cttcgtcaat ttcggtttgg atcacgcgct catctatacc
    13621 ttcgtagcga ccgcacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc
    13681 ttgatcaagc ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc
    13741 ggcttttgct gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc
    13801 gccgccgtaa ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact
    13861 ccaactctgg atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt
    13921 aattgcgcgg aacatttcag gaaacaggct aattatgcca atccacatag cgccgtcttt
    13981 taccgtttat ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt
    14041 gagatcgact ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc
    14101 aaacgcatct ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat
    14161 atcgacgact ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc
    14221 tttccagtag tagtccccct cttcaagctg aggcagctgc gatgaatcca cgacaatttc
    14281 acaattcgtc agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat
    14341 gtcctgattg tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg
    14401 gataaaccag ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct
    14461 gagccaccca cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg
    14521 tgcttgcgcg gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt
    14581 tacttctttg atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg
    14641 agcgatgcga tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa
    14701 gaaaccaacg cgctcgatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac
    14761 ctggtagaac ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc
    14821 ctcttgtgtg aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa
    14881 agcccgaaaa ttttactcat ttttgcggga attgcaatca acagttgcta actctgctgt
    14941 aaaaggccgt cggcggtgca gccagtttgg tgccggagtg cgcgcagtca ccggagcgta
    15001 cacgcagtac gtgaggatga cgagcacatc ccggtgccaa aatggcaaac aagccaggcc
    15061 gattagcgac cagggaagcc tgggggcatc atacccttca tgcttctcat catcttcgcc
    15121 attccgcctt tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg
    15181 cggttcacgt cctgcacctg cataccgcaa ccggcagcga tacgacgctt acgcgaacct
    15241 ttgatgattt ctggcttagc gcgctctttt atcgtcatcg agttgatgat ggcttccata
    15301 cgcaccagca ctttatcgtc catctgcgac ttaacattat ccgggatctg ccccatgccc
    15361 ggcagcttgc ccatcaggct agccatgccg cccatatttt tcatctggcg cagttgctca
    15421 agaaaatcgt tgagatcgaa gccgtcgcct tttttcagct tgctggctaa tttctctgcc
    15481 tgcgcgcggt caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg
    15541 agaatacgcg acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca
    15601 acaccgagaa acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg
    15661 cgggcatcgc cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat
    15721 gcttttgccg tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca
    15781 accgggttaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
    15841 agacgaccag cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
    15901 gccgcgttaa cgatatctac cggcttctga ccaacatcag aagggaagaa atcaacgccc
    15961 acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgataaac gtcggcagaa
    16021 accaccagca ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg
    16081 gttgttttac cggcaccttg caggcccgcc atcagtacga ccgcaggcgg ttgcgcagcc
    16141 aggttcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gactattttg
    16201 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
    16261 acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg
    16321 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
    16381 ttgcgcagcg tgcgcgacaa acgatcagtt aaattatcaa acattgtctc tcgcctgggg
    16441 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
    16501 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
    16561 tttcttattg ctcaaactgt caacattact atgcccgttt ttgctctgct cgcgcttgtc
    16621 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
    16681 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
    16741 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
    16801 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
    16861 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg
    16921 cccaatgaat acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta
    16981 tcgctctttt cctatgccac gctgataatt gccgccctgt acgcgctaca actggcgtgg
    17041 attgattacc aactgaagaa caagaagctg gcgtttaacc aagaaatgcc gccattgatg
    17101 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctact aacgctcacg
    17161 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggcc
    17221 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa
    17281 ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg gtgcagtcat cctgacactg
    17341 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
    17401 ccctggaaca catttctact actacgttga tcattattct gatcatcatg gtggtcattt
    17461 cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtctgcgac
    17521 atatggcgaa acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag
    17581 accgcctgat aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc
    17641 tcggcactat tgttgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg
    17701 tgctgacttt tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt
    17761 acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga
    17821 tgccgctggt ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca
    17881 aaaccgatat cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcacg
    17941 aatcacgctc acaaatatcc cgtcgcaatc aggatatgct actgtctgtg ctcgatctgg
    18001 aaaagatgac cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca
    18061 acgatgactg gaaatcgatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc
    18121 tctaccgtga ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt
    18181 tgatgtcgga gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct
    18241 attttgtgcc ggaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa
    18301 agaaagtcgg cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag
    18361 atattctgga agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag
    18421 aggtcacgcc gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa
    18481 tcaacaaagc ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca
    18541 ttcttgaggc actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcgagtacg
    18601 atatagatat tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga
    18661 aaccgctgcg cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc
    18721 gtgaattatt acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca
    18781 cgaatcgtac gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc
    18841 gcaacgtcat cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg
    18901 ttagtttcgg cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc
    18961 gtcagctcaa tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc
    19021 agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg
    19081 aatttgtggg ctttttcaat atccagttca gtacgtcgac gcaggttttc catttcggct
    19141 tttacacgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga
    19201 ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc
    19261 tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc
    19321 tgttctttac tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc
    19381 ttattatggg gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg
    19441 caacaaggac ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc
    19501 acggcacccc actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg
    19561 ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac
    19621 tggcacgctc gcggagattg ggcaactagc tgatctcgcg gtagtcgttg gtggcgacgg
    19681 taatatgctg ggcgcggcac gcacactcgc ccgttacgat attaaagtta ttggaatcaa
    19741 ccgtggcaac ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacagttagc
    19801 cgatgtgctg gaaggccact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg
    19861 tcagcaagat tgccagaaac gcatcagcac cgcgataaat gaagtggtgc ttcatccagg
    19921 caaagtggcg catatgattg agttcgaagt gtatatcgac gagatctttg cgttttctca
    19981 gcgatctgat ggactaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc
    20041 aggcggtcct attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca
    20101 tacgttgtca gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc
    20161 gcatcgccgt aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga
    20221 aggtgaagat gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga
    20281 ttacagttat ttcaacacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt
    20341 ttacgccagc ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg
    20401 gtttttcata caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg
    20461 ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggcg
    20521 cgggtaaatc tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg
    20581 acatggtgcg taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata
    20641 cgccagcggc tctgcgctgg ctggaagaaa atcagcttga agacgggcat gaatgtttgc
    20701 ttcgtcgagt gatcagcagc gatggtcgct cccgtggttt catcaacggt acagctgttc
    20761 ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc
    20821 atcaattact caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa
    20881 cctctctact gcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc
    20941 tcgcgcatca tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc
    21001 aattaaaaga acttaacgaa tttaatccgc agcccggaga gtttgaacaa atcgacgaag
    21061 agtacaaacg tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat
    21121 taatggccga cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg
    21181 tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag
    21241 ctaccatcca gattgctgaa gccagcgatg aactgcgcca ctactgcgat cgtctggatc
    21301 tcgatcccaa ccgactattt gaacttgaac agcgcatctc gaaacagatt tcgctggcac
    21361 gtaaacatca cgtcagccct gaggcattgc cacagtatta ccagtcgcta ctggaagaac
    21421 agcagcaact ggacgatcag gccgactcac aagaaacgct tgcgctggcg gtaacgaaac
    21481 atcatcagca ggcactggaa atcgcgcgcg cattacacca acaacgccag caatatgcag
    21541 aagaacttgc acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt
    21601 ttacgatcga tgttaaattt gacgagcatc acctgggcgc tgacggtgcc gatcgtattg
    21661 agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg
    21721 gtggtgaatt gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa
    21781 ccccggcact gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg
    21841 tcggcaaact gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc
    21901 cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga
    21961 tgacagaaac gcatatgcaa tccctgaata aaaaagcgcg gttacaagag ctggcgcgcc
    22021 tgcttggtgg cagtgaagtc acacgtaata cactggcgaa tgcgaaagaa ctgcttgcag
    22081 cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa
    22141 gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg
    22201 tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca
    22261 gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt
    22321 cctgacatca accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc
    22381 atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt
    22441 acgaatacct ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa
    22501 acgctgacgc tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg
    22561 ctgagtggta actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc
    22621 accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt
    22681 tcggatcggc aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc
    22741 ttagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac
    22801 gcaattccag caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg
    22861 tcacgcgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac
    22921 ctggcactgt aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agacgccagc
    22981 tctttaaaca cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga
    23041 tgaaactcga tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat
    23101 ttcttgaacg gcccatccac cagattcata agaatacttt ggttactggt caactggttg
    23161 cgggtagtaa acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc
    23221 ccaggagtgg actccagaat ccgacttccg gtacaacccg gcaaaaactg aggataagac
    23281 tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg
    23341 ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct
    23401 gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc
    23461 ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac
    23521 gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg
    23581 cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg
    23641 ggaggttaaa tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg
    23701 tgacggagag gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca
    23761 tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc
    23821 attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa
    23881 aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg
    23941 ttcagatatc aaagaacgcg aatggcaggt ggataaagca cgtatcatga aaaacgccca
    24001 ccgttaaacc tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc
    24061 gcgaatgaac atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa
    24121 tcgaataaaa atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg
    24181 tcatggcgct cataaatctg gtatacttgc ctttacacat tggggctgat tctggattcg
    24241 acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca
    24301 aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc
    24361 tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc
    24421 gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg
    24481 cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga
    24541 tgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accactttaa gaaggactac
    24601 aaccggacag tagcaataaa tacagccact tacggacaca agccagacag tagtcggact
    24661 gaaaaaagac aaaaatatac acgtgaaatg cacgtgcact tctaaaaacc cctagttatc
    24721 accgactggg gtttttcaat ttgtaactaa gggtaacaaa aacacccccg tttttttcat
    24781 ctcactcacc ttgatactgt ttatttgtac agttaaaaat aatactgtat acaaacacag
    24841 tacagaggga cttttatgcg tattgagatc tgcatagcca aagaaaaaat gactaaaatg
    24901 ccaaacggtg ctgtggatgc gttaaaggaa gaattaaccc gacgcatcag taaacgttat
    24961 gacgatgtag aggtgatcgt aaaagccacc agcaacgatg gcctttctgt tacgcgcacc
    25021 gccgataaag attctgcaaa aacttttgtt caggagacgc tgaaagatac ctgggagtct
    25081 gctgacgagt ggtttgttcg ctaataaaca cgtaaatcgg taacggctgg aaatcattca
    25141 atactcgcac tatcgaaagt tcaccagcca accgcgacac gctcttacat acgaagtgcc
    25201 gcgctttcct taataatttt ttagcagtac tgtgtaaata atgagcgacc taatccatgc
    25261 atgacgcgta gtgcctattg tgcatctttc tgcgtctctt tttactggac ccaagccaat
    25321 gatgctggcg ataccattcc cgccaggccc acttacaaca gtaaaattcg gagctctgcc
    25381 tttacataca atac
//
LOCUS       DAZUFB010000062        25292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZUFB010000062 DAZUFB010000000
VERSION     DAZUFB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.062"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..608)
                     /locus_tag="ACHY3M_002925"
     CDS             complement(<1..608)
                     /locus_tag="ACHY3M_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFS4317804.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIDALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFN"
     gene            complement(960..1124)
                     /gene="yjiS"
                     /locus_tag="ACHY3M_002926"
     CDS             complement(960..1124)
                     /gene="yjiS"
                     /locus_tag="ACHY3M_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFS4317805.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            1301..2713
                     /gene="yjiR"
                     /locus_tag="ACHY3M_002927"
     CDS             1301..2713
                     /gene="yjiR"
                     /locus_tag="ACHY3M_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4317806.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGISISTVQ
                     QAYQTLETMKFITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGDAVYRAME"
     gene            complement(2958..3914)
                     /locus_tag="ACHY3M_002928"
     CDS             complement(2958..3914)
                     /locus_tag="ACHY3M_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS4317807.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            4381..5613
                     /gene="mdtM"
                     /locus_tag="ACHY3M_002929"
     CDS             4381..5613
                     /gene="mdtM"
                     /locus_tag="ACHY3M_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFS4317808.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5654..6934
                     /gene="yjiN"
                     /locus_tag="ACHY3M_002930"
     CDS             5654..6934
                     /gene="yjiN"
                     /locus_tag="ACHY3M_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFS4317809.1"
                     /translation="MNKLVELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRNAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSLQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            7050..8201
                     /gene="yjiM"
                     /locus_tag="ACHY3M_002931"
     CDS             7050..8201
                     /gene="yjiM"
                     /locus_tag="ACHY3M_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFS4317810.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            8211..8978
                     /gene="yjiL"
                     /locus_tag="ACHY3M_002932"
     CDS             8211..8978
                     /gene="yjiL"
                     /locus_tag="ACHY3M_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFS4317811.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGTRFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            8975..9232
                     /gene="ytiA"
                     /locus_tag="ACHY3M_002933"
     CDS             8975..9232
                     /gene="ytiA"
                     /locus_tag="ACHY3M_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFS4317812.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            9297..10157
                     /gene="yjiK"
                     /locus_tag="ACHY3M_002934"
     CDS             9297..10157
                     /gene="yjiK"
                     /locus_tag="ACHY3M_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS4317813.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            10225..11403
                     /gene="yjiJ"
                     /locus_tag="ACHY3M_002935"
     CDS             10225..11403
                     /gene="yjiJ"
                     /locus_tag="ACHY3M_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4317814.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(11416..11970)
                     /gene="kptA"
                     /locus_tag="ACHY3M_002936"
     CDS             complement(11416..11970)
                     /gene="kptA"
                     /locus_tag="ACHY3M_002936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFS4317815.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            12220..12903
                     /gene="yjiH"
                     /locus_tag="ACHY3M_002937"
     CDS             12220..12903
                     /gene="yjiH"
                     /locus_tag="ACHY3M_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFS4317816.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12900..13361
                     /gene="yjiG"
                     /locus_tag="ACHY3M_002938"
     CDS             12900..13361
                     /gene="yjiG"
                     /locus_tag="ACHY3M_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFS4317817.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTVHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            13374..14546
                     /gene="iadA"
                     /locus_tag="ACHY3M_002939"
     CDS             13374..14546
                     /gene="iadA"
                     /locus_tag="ACHY3M_002939"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4317818.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            14611..15522
                     /gene="hypT"
                     /locus_tag="ACHY3M_002940"
                     /gene_synonym="qseD"
     CDS             14611..15522
                     /gene="hypT"
                     /locus_tag="ACHY3M_002940"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFS4317819.1"
                     /translation="MDDCGAILHNIETKWLYDFLPLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSNYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSRKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(15515..15907)
                     /gene="iraD"
                     /locus_tag="ACHY3M_002941"
     CDS             complement(15515..15907)
                     /gene="iraD"
                     /locus_tag="ACHY3M_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFS4317820.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPLSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(15904..15987)
                     /gene="idlP"
                     /locus_tag="ACHY3M_002942"
     CDS             complement(15904..15987)
                     /gene="idlP"
                     /locus_tag="ACHY3M_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFS4317821.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(16103..16246)
                     /gene="ytiD"
                     /locus_tag="ACHY3M_002943"
     CDS             complement(16103..16246)
                     /gene="ytiD"
                     /locus_tag="ACHY3M_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFS4317822.1"
                     /translation="MADYAEINNFPPELSSGGDKYFHLHNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            16579..17409
                     /gene="yjiC"
                     /locus_tag="ACHY3M_002944"
     CDS             16579..17409
                     /gene="yjiC"
                     /locus_tag="ACHY3M_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS4317823.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELIRKYERARLDTENIY
                     LLPLVRGNNHNYDGKSVVEIRKLDISKEYWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(17550..18323)
                     /gene="uxuR"
                     /locus_tag="ACHY3M_002945"
     CDS             complement(17550..18323)
                     /gene="uxuR"
                     /locus_tag="ACHY3M_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFS4317824.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(18538..19998)
                     /gene="uxuB"
                     /locus_tag="ACHY3M_002946"
     CDS             complement(18538..19998)
                     /gene="uxuB"
                     /locus_tag="ACHY3M_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFS4317825.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(20079..21263)
                     /gene="uxuA"
                     /locus_tag="ACHY3M_002947"
     CDS             complement(20079..21263)
                     /gene="uxuA"
                     /locus_tag="ACHY3M_002947"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4317826.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            21603..22946
                     /gene="gntP"
                     /locus_tag="ACHY3M_002948"
     CDS             21603..22946
                     /gene="gntP"
                     /locus_tag="ACHY3M_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFS4317827.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISVVA"
     gene            complement(23196..24098)
                     /gene="fimH"
                     /locus_tag="ACHY3M_002949"
     CDS             complement(23196..24098)
                     /gene="fimH"
                     /locus_tag="ACHY3M_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS4317828.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(24118..24561)
                     /locus_tag="ACHY3M_002950"
     CDS             complement(24118..24561)
                     /locus_tag="ACHY3M_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4317829.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(24634..25164)
                     /gene="fimF"
                     /locus_tag="ACHY3M_002951"
     CDS             complement(24634..25164)
                     /gene="fimF"
                     /locus_tag="ACHY3M_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFS4317830.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
BASE COUNT         5895 a         6431 c         6378 g         6588 t
ORIGIN      
        1 tgattaaata ccgccatgcc ttgtgataca ccatatttat cttttaaagc cgttagcagt
       61 gccagattat caatttcgct agtgctatca gagaagcggt ttgccatggt gcccacaaat
      121 atcatcgcga catcaaacgg ttcccagcgc ttaggagtaa agccaaatgt attaaactgt
      181 tttggtaaga gcgtctctgg attggtattt accttatcaa tccaggcatt cattccatca
      241 gcgtagcctt gcagaatgga catatcctct ggggaaaggg catcaatttg cgcccggata
      301 gcatccggcc agtagttacg acggatatct ttatcaaatt tcacaaaatc tttgccaagc
      361 acttccgcga cagtcccttg agtactgcga cgtgccattt ccatctgaaa aaggcgatct
      421 tgtgctacta catagccata gccataaaat aggtgccatg tatcattggc ataaatatgc
      481 ggcatgccgt attcatcgcg aacaatcttt atctcacttg acgactgctc agccagtgca
      541 ggtaagctcc aataatacat cagggaagca gtaacacagt tcacgatcat acgatttcta
      601 tttttcattg tatcctctgg caggtgtata attagcgaat tgatactagc aacgaagctt
      661 cggttatcta tttgcagaga attgtttcat tatgaactgt gagcgcgata actaattttt
      721 gatacaaaaa tttcattaat gtgatctaaa acgaatcgct ttattcaccg ctgatgattt
      781 tgttctattc ctttttgtga cagatccccc ggacgcatag atgatgaccc ccagagagac
      841 agctcaggtc attatctgta tgtaaatcag aaaaatatgt tgtaaacgac ttttatggtt
      901 ggattttgta gcaattttga atgcagacag gcagcatttg ccaacacgcc gcccatagtt
      961 cactccacat cctccctgcg taacccgata tccctcaacc gttcatcact catctgctgt
     1021 aacacccgtc gggcctgcat ttgccgccgc caacgcctca ccgcctgcca gagttgcaca
     1081 agtctgataa acggcgtttt ggctttgttt tcgtgaaatt ccatctgctg cctcctcatc
     1141 tttcagaggc ttcatcatcc gccttctggc agttcacaat acagatgcaa atattagttt
     1201 tatttaacat acagacaggt taaaacagca tctgcatccg gttttttagc ctcatctgta
     1261 ccggtttttc tttctgtatg ggccaaataa ggaatacagc atgacgcgtt atcaacatct
     1321 ggcgactcta cttgccgagc ggattgagca agggctgtat cgtcacgggg agaaattgcc
     1381 gtcggtgcgc agcttaagtc aggagcacgg catcagcatc agcaccgtgc agcaggcgta
     1441 ccagacgctg gagacgatga agttcatcac tccgcagccg cgttcgggtt attttgtcgc
     1501 acaacgtaaa gcccagccgc ctgtaccgcc gatgacgcgt ccggtgcagc gcccggtgga
     1561 aattacccag tgggatcagg tgctggatat gctggaggcg catagcgaca attccattgt
     1621 tccgttaagc aaaagcacgc cggatgtcga aacgcccagc ctgaaaccac tctggcgtga
     1681 gctaagccgg gtggtgcagc ataatctaca aaccgtgctc ggttatgact tgttagccgg
     1741 tcagcgagtg ttgcgggagc agattgcccg cctgatgctc gacagcggct cggtggtcac
     1801 cgccgatgac atcatcatca ccagcggctg ccataactcg atgtcgctgg cgttaatggc
     1861 ggtgtgtaaa ccgggcgata ttgtcgcggt cgaatctccc tgttattacg gttcgatgca
     1921 gatgctgcga ggcatgggcg tgaaagtgat tgaaatccca accgatccag aaactggcat
     1981 cagcgttgaa gcactggaac tggcgctgga acagtggccg attaaaggca tcattctggt
     2041 gccaaactgt aataatccgc tgggatttat tatgccggac gcacgcaaac gggccgttct
     2101 ctctctcgct cagcgtcatg atattgtgat ttttgaagat gatgtctacg gcgaactggc
     2161 aacggagtat ccgcgcccgc ggaccatcca ttcatgggat atcgacgggc gagtgctgtt
     2221 gtgcagctcg ttcagtaaaa gtattgctcc aggcctgcgc gtgggttggg tcgcaccggg
     2281 gcgttatcac gataaactga tgcatatgaa atacgccatc agcagcttta atgtgccgtc
     2341 cacgcaaatg gcggcggcaa cgtttgtgtt ggaaggccac tatcatcgcc atatccggcg
     2401 gatgcggcag acttatcagc gcaatctggc gctttatacc tgctggatac gggaatattt
     2461 tccctgcgaa atctgtatta cgcgcccgaa aggcggattt ttactgtgga tcgaattgcc
     2521 tgaacaggtc gatatggtct gcgtcgcgcg gcagctgtac cgcatgaaaa tccaggtggc
     2581 ggcaggctcg attttctcgg cttccggcaa ataccgtaat tgtctgcgca tcaactgcgc
     2641 tttgccgctc agcgaaacct atcgcgaagc actaaagcaa attggcgatg ccgtgtatcg
     2701 ggcaatggaa taaattcgaa ttagtgccga ggcccctgat ttactctcac gaataacctg
     2761 tgactcgttt tatctttata gcgacatcgc cacgttccgg catggcttag gcaaagtgtg
     2821 cttcgttcat gccagatgcc atgtgaacgc cttaccaggt ctacaaaata ttaaaagttc
     2881 aatatattta atggattatg tagatacctg ataagcgtag cgcatcaggc tgctttgcgt
     2941 ttgtcatcag cctctgatta gtactcgatc catggttcgc gcttgctttc aggcaagggc
     3001 tgatctaatg cctgcatttg ctctgccgaa agtccggtat atcgttgtat ccattcagga
     3061 tcggccccat tctgcaacaa caatcggagg aggctccgtt ccccttctac tttcccttct
     3121 ttcctccccc tctcccttcc caacagccat ccatcattat gaattcgctc tgcaattgtc
     3181 attattcgct ccctgtggtg tggcattcgc ctggtaagtt cgctgataaa cgccttaaaa
     3241 cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta
     3301 gcacactctg taaccagtaa tactaccagt tggtcaataa gccccatcag atcgcgctgg
     3361 cgaatatgct tttggatcaa ctccaacagg gcgactctgc gatgctgcac aatctcgtcg
     3421 tctggcacga cagtgacatc caccagcggg aacgctgcgc tataaagttt ccgtgcggta
     3481 gtcggggcgg caaattcgtc cagccaacac agggaccagg ggtaaggact acggctaccg
     3541 tgataaaaca gcatcgggat aaccagcggt agcggccggc gtttatcatg ctcgatatgg
     3601 cgctgcatca ccgccatgga atatcgcatc aggcgaaacg ccatatgaat atcctcgcgg
     3661 ctctgatgtt caatcactac gtaaatataa ccatcacctt cacgggtctt taccgaccac
     3721 agaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga ttccagtttt
     3781 aagctgtcga gatcgcacag ttcacgtaaa tctttaggta agtggatctc cataaaatcc
     3841 cgcgcggtgc cagggtgcgt gagaaaggac ttaaataatg cgtcatgcgg cgtgctggtc
     3901 gagaagtttg tcatggcgat ccgtcacctg tagataagat ggcccgactt tagcggctaa
     3961 caaaaccatt ctcacccggc aatttcccgt tccccgaggc gctttccagg ctatttttat
     4021 tcttctgcaa acgatacatt ttttcggaaa caggctcgcg ttttacattt cctgtacccg
     4081 gcagatattc ccctttccac cgcgctcttt tgattaaccg caaattttaa ttacacttaa
     4141 ggtgtatatt ttctatgcaa cccatcaatt aaagaggtgt aatgtgctga tgactatttg
     4201 taatcgttat acattctgac ctggagtcag aaggtatttc tctgtctgtg tgttcacagg
     4261 cagtgtggtt gattacatga aattcagtac atttgtcctt ctcgcctgcc tttcgtcatt
     4321 accgacggta ttgaatttcg ttttccccgt tggggttctc cggacaagga gttgtttgtt
     4381 atgccacgtt tttttgcccg ccatgccgcc acgctgtttt tcccgatggc gttgattttg
     4441 tatgactttg ccgcgtacct gtcgacggat ctgatccagc cggggatcat taatgtggtc
     4501 cgtgatttta atgccgatgt cagtctggcc ccggcagccg tcagtctcta tctcgcgggc
     4561 ggtatggcgt tacagtggct gctggggccg ctttccgaca ggattggccg caggccggtg
     4621 ctgattaccg gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct
     4681 atgacacagt ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgccacc
     4741 gttggttatg tcacggtgca ggaggcgttc ggccagacaa aagggatcaa gttgatggcg
     4801 attatcacct ccatcgtact gattgcgccg attattggcc cgctttccgg cgcagctctg
     4861 atgcactttg tgcactggaa agtccttttt gccatcattg cagttatggg ttttatctca
     4921 tttgttggct tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc
     4981 gccaaaagcg tctttcgcga ttttcgtaat gtcttttgca atcggctgtt cctctttggc
     5041 gcagcaacca tctctttaag ctatatccca atgatgagtt gggtggctgt ctcgccggtg
     5101 atcctgatcg acgcgggcgg cttaacaact tcgcagttcg cctggacaca ggttccggtg
     5161 ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa
     5221 ccgcgcttta tctggcgtgc tgtacccatt caactggtcg gcctcgcact gttgattatc
     5281 ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tttgtatgct
     5341 ttcgggattg gtttgatctt cccgacctta ttccgcttta cgcttttttc caataactta
     5401 ccgaaaggga ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg
     5461 gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc
     5521 gtcgtggcgg gcgttatcgt cgttttcacc ctggcaggat tgcttaatcg cgtgcgccaa
     5581 catcaggctg ccgagctggc ggaggagcgg tgatttttgc gcgatcctgc cgtcaggctc
     5641 tattcttaac gttatgaata aactcgttga actcagacgc gccaaaatgt tggcgctctc
     5701 tttactgctt atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg
     5761 ggtgagcggc gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt
     5821 tgcggtggtg gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat
     5881 tatcccgcgt aataaagacc ggattggcga aaatctcggc cagttcgtgc aggaaaaatt
     5941 tctcgatacc cagtcgctgg tggcattgat tcgacgtcac gaaccggcgt tgctgattgg
     6001 caactggttt agccagccag aaaacgcccg ccgcgttggt cagcatctgt tgcagatcat
     6061 gagtggtttt cttgaactga ccgatgatgc gcgtattcag cgcctgctta agcgcgcggt
     6121 ccatcgggcg attgataagg tcgatctttc cggaaccagt gcgttgatgc tggagagtat
     6181 gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg atcgcacagt tgatcgccct
     6241 tctccagcgc gataaatcgc gcaagtttat tgcccagcag attgttcgct ggctggagag
     6301 tgagcatcca ctgaaagcca aaattctccc caccgaatgg ttgggcgaac atagcgcgga
     6361 gttggtttct gacgcggtga attctttgct tgatgatatc agccgtgatc gtgcgcatca
     6421 gatccgtaat gcgtttgatc gcgccacttt tgccctgatc gacaagttga aaaacgatcc
     6481 ggaaatggca gcgcgagccg atgccgtaaa aagttatctg aaagaagatg aagcttttaa
     6541 ccgctatctc agtgaattgt ggggggattt acgggagtgg ctgaaagcgg atatcaacag
     6601 tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt caatggtttg gcgaaacgtt
     6661 aattgccgat gatgccttgc gggcgtcgtt aaatggtcac ctggaacaag ccgcgcaccg
     6721 cgtcgcgcct gagttttccg cattcctgac gcgccacatc agcgatacag taaaaagctg
     6781 ggatgcgcgg gatatgtcgc tgcaaatcga gttaaatatc ggcaaagatc tgcagtttat
     6841 ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta attttatatt tgttgtcgca
     6901 gctcccggcc ttgttccccc tcggcaattt ttagaaatcc atgaaaaact aagtggctaa
     6961 ccgcgagaga gatcaaataa accacactaa aagaatggta ataatgtggc ctcttttcat
     7021 tatctcccgt ggtacgggga aggaaaatca tgtcacttat caccgatcta cccgccattt
     7081 tcgatcagtt ctctgaagct cgccagaaag gctttctcac cgtcatggat ctcaaggagc
     7141 gcggcattcc gctggttggc acttactgca cctttatgcc gcaagagatc ccgatggcag
     7201 ccggtgcggt tgtggtttcg ctctgttcta cctctgatga aaccattgaa gaagcggaga
     7261 aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag ctacggcttc ggcaaaaccg
     7321 ataaatgccc ctacttctac ttttcggatc tggtggtcgg tgaaaccacc tgcgacggca
     7381 aaaagaaaat gtatgaatac atggcggagt ttaagcccgt tcatgtgatg cagttgccga
     7441 acagcgttaa ggacgatgcc tcgcgtgcgt tatggaaagc cgagatgctg cgcttgcaaa
     7501 aaacggtaga agaacgtttt gggcacgaga ttagcgaaga tgctctgcgc gatgccattg
     7561 cgctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta tcatcttggg cagttaaatc
     7621 ctccggcgct tagcggcagc gacattctga aagtggtcta cggcgcaacc ttccggttcg
     7681 ataaagaggc gttgatcaat gaactggacg cgatgacagc ccgcgttcgt cagcagtggg
     7741 aagaaggcca gcggctggcc ctgcgtccac gcattttaat caccggctgc ccgattggcg
     7801 gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg cggctgggtt gtcggttatg
     7861 aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc agaaacgggc gatgtctacg
     7921 acgcactggc ggataaatat ctggcgattg gctgctcctg tgtttcgccg aacgatcagc
     7981 gcctgcaaat gctcagccag atggtggaag aatatcaggt cgatggcgta gttgatgtga
     8041 ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc gattaaacgt catgtgcgtc
     8101 agcagcacaa cattccttat atcgctattg aaacagacta ctccacctca gatgtcgggc
     8161 agctcagtac ccgtgtcgcg gcctttattg agatgctgta aggagtggca gtgacatatt
     8221 cgattggcat tgattctggt tctaccgcca ccaaagggat cttactggca gacggcgtga
     8281 ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc aacagcaatt actgaagcct
     8341 gggaaactct gcgcgaaggg ttagagacaa cgccgtttct gacgctcacc ggctatggac
     8401 ggcaactggt ggattttgcc gataaacaag tgacggaaat ctcctgtcat gggctgggca
     8461 cacggtttct tgcgccagca acgcgcgcgg taatcgacat cggtggtcag gacagcaaag
     8521 tgattcagct tgatgatgac ggtaacctgt gcgatttcct gatgaatgac aaatgcgcgg
     8581 cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct tggcaccagc gtcgagcaac
     8641 tcgacagcat taccgaaaat gtcacgccgc acgccatcac gagtatgtgc acagtgtttg
     8701 ccgaatcaga agtgatcagc ctgcgctcag cgggcgtcgc gccagaagcg attctcgccg
     8761 gagtgattaa cgcgatggcg cggaggagtg ccaatttcat tgctcgtctc tcctgtgaag
     8821 cgccgattct gtttactggt ggcgttagtc attgccagac gtttgcccgg atgctggaat
     8881 ctcatctggg aatgccggta aatacccatc ctgatgcgca atttgctggc gcaattggcg
     8941 cggcggtaat tggtcaacga gtgaggacac gccgatgaaa gagtttttat ttttatttca
     9001 ctccacggtc ggcgtcatac aaacccgcaa ggcgttgcag gcagcgggca tgacttttcg
     9061 cgtcagcgat attccccgtg atttacgcgg cggctgcggg ttatgcattt ggctgacctg
     9121 tccccccggc gaggaaatac aatgggtgat ccctgggcag actgagtcaa tttattgcca
     9181 gcaggatggt gtctggcgct gcattgcaca ttacggggtt tctccgcgtt aaggattcat
     9241 taaggtcgct acgtatactt ctcgatatct tattgatttg ccttaaaggg attaccgtga
     9301 caaaaagtat ttcgttaagt aaacgcatct ccgttattgt gattcttttt gccattgtgg
     9361 cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag caatcatgca gcatcatttc
     9421 agaattacca cgccaccatt gacggcaaag aaattgcagg cataacgaat aatatctctt
     9481 cgctgacctg gtcggcacaa agcaatactc tttttagcac tatcaacaaa cctgccgcca
     9541 ttgttgaaat gaccaccaat ggtgatttta tccgcacaat tcctctggat tttgttaagg
     9601 atctcgagac gatcgaatac attggcgata atcagtttgt cattagcgat gagcgcgatt
     9661 acgccattta tgtgatttca ctgaccccaa actcggaagt aaaaatcctc aaaaaaataa
     9721 aaatcccgct gcaagactcc ccaaccaatt gcggttttga agggttggct tattctcgcc
     9781 aggatcacac tttctggttt tttaaagaga aaaatccgat tgaggtctac aaagttaacg
     9841 ggctgttaag tagcaatgaa ttacacatca gcaaagacga ggcactacaa cggcaattta
     9901 ctttagatga tgtgtccggc gcagaattta accagcaaaa aaatacgctt ctggtgctgt
     9961 ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg cgaggttatc ggtgaaatat
    10021 cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa acaggctgaa gggattgcga
    10081 tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa tcgtttttat cgctttaccc
    10141 ctaaatcatc gcattaacag gctggcatat ttcacctttt tgttattaac ttgttaacaa
    10201 caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc atcccgtaga acgcttttct
    10261 ttcagcaccg cgcttttcgg gatgctggtt ctgaccttag gtatgggatt aggtcgcttc
    10321 ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg cgttttcatt tagccagctc
    10381 tcgtggattg ccagcggcaa ctatgcgggg tatctggctg gcagtctgct attttcgttt
    10441 ggcgcatttc accagccgtc gcgcctgcgc ccatttctgt tggtttctgc cctagcgagc
    10501 gggttgttga tcctcgcaat ggcatggttg ccgccattta ttctggtgtt attgattcgc
    10561 ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg gttcgacgct gattatgcag
    10621 cacacgcgcc atccttttgt gctggcagct ttgttttctg gcgttggcat tggcattgca
    10681 ctgggcaacg aatatgttct ggcaggcctg cattttgcct tatcctcgca aacgttatgg
    10741 caaggcgccg gggcgctttc tggcatgatg ctgattgcac ttacgctttt aatgccctcg
    10801 aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg agcaacagat aatgagctgg
    10861 tggttactgg ctattctgta cggcctggcg ggatttggtt atatcatcgt cgcgacttac
    10921 ctgccgctca tggcgaaaga tgcaggctca ccattgttaa ccgcccatct ctggacgtta
    10981 gtcggcttat caattgtgcc aggctgcttt agctggctat gggcagcaaa acgttggggg
    11041 gctttgcctt gcctgacggc gaatttgctg gtgcaggcta tcagtgtgct gcttactctc
    11101 gccagcgact ctcctctctt gcttatcatt agtagtatcg gttttggcgg tacctttatg
    11161 ggcacgacat ctctggtgat gactatcgcc cgccaactga gcgttccggg aaatctcaat
    11221 cttttaggct ttgtgacgct catttacggc attggacaaa tcctcggccc ggcgctgacc
    11281 agtatgctcg gcaatggaac atcggcgctt gccggcgcca ctctctgcgg cgcggcggcg
    11341 ttgtttattg ctgcacttat cagcacagta caattattta agctgcaagt ggtcacttca
    11401 tgaggagtgt ttcccttacc actcaagaaa ttcgaccgca accgttgatg tcagccagac
    11461 tccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg ctttgacggt
    11521 taagataacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt catcagcaga
    11581 gagatgcacg taatgacgct cccctgcaat cagcccctgt ttttttatct cgtcaaggaa
    11641 acggcttgcc gtaccgtggt aaagaaactg tggcggtgtt ttttcggcaa aggaaatggc
    11701 gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac tggaataact
    11761 aaaacgcttt ttatcactgg tagcgaccac ggtatccagc aatgcacggg ttaaccgctt
    11821 gcccgctttt tgggcacaga gtataagctt atcgatatcc gcccatcctt cacggtcaag
    11881 tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact tgctcgtatc
    11941 cgccaactct ttttcgttgt actttgccat ctcgttttcg tccctggatt ttctgttttt
    12001 atttactgat gtcattgtca ctgggtttat gaattttctg caactcaatc ctgcgcagag
    12061 ccttttccac acagcataat ttttccgcta tgcaaaaaac gcatagccag aaacatactt
    12121 gcattttccc catacccaca tggctgataa ggtttttccg ctccaggaat tttcctggct
    12181 caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca aggggatgct
    12241 gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc tttttttctc
    12301 accataatct ttttttccgg tgttttttcc ggaacagaca gctggtggcg cgtttttgat
    12361 ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaacggagc caccacctca
    12421 tttcgcgggg cgggtggcgc gggggctaaa gatggctttc tctttgcgct ggagctggca
    12481 ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg tggattacgc
    12541 gccgcgcagc agttaatgac gcctgtgcta aaaccgttgt tgggcattcc agggatctgc
    12601 tcgctggcgc tgatcgctaa cctgcaaaac acggatgccg ccgccggtat gaccaaagag
    12661 ctggcgcagg aaggggaaat taccgaacgc gataaagtga ttttcgccgc atatcaaacc
    12721 agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt tgcctttctg
    12781 ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt cggagccaac
    12841 attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca
    12901 tgacaactca ggtccgcaaa aatgtcatgg atatgtttat cgatggtgcg cgccgtggct
    12961 ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc atcattcagg
    13021 cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc
    13081 tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg agtatgggcg
    13141 gcgcagtcgg tgttgccgcc agtctggcga ccgccggcgc attaaccgtt cacgatgtca
    13201 ccgttttact gcccgcgatg tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc
    13261 tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc tgcgtgatta
    13321 acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt accatgattg
    13381 attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg ccggaagatc
    13441 ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc
    13501 cttctgacat tgtaccggac tgcacggttg tcgatctcag cgggcagatc ctctgtcctg
    13561 gttttattga tcaacacgtc catttgattg gtggtggcgg cgaagcaggt cccacgacgc
    13621 gcacgccaga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca gtggttggtc
    13681 tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag acccgtgcgc
    13741 tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca
    13801 ccattacagg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat
    13861 gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct aatatggcgg
    13921 cggaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg ttccacatgg
    13981 gcgacagcaa aaaggcgtta cagcctgtct atgacctgct ggaaaactgc gatgtgccga
    14041 tcagcaagtt gctgccgacc cacgttaacc gcaacgttcc gttgtttgag caggcgctgg
    14101 agttcgcgcg caaaggcggc actatcgata tcaccagcag cattgacgaa ccggtcgccc
    14161 ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc gtcaccctca
    14221 gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta acccatatcg
    14281 gtgttgccgg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt
    14341 tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc cttaacctga
    14401 ccgggaaagg cgaaattctg ccaggcaatg atgctgactt gctggtcatg acgccagaac
    14461 tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc aaagcctgcg
    14521 tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg cccgaatgga cgtagaatgc
    14581 ccgcaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcgat tttgcataat
    14641 attgaaacca aatggcttta tgattttctg cccctggaaa aatgccgcaa tttttcccag
    14701 gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg tgcgctggaa
    14761 caggcgattg gtgttgaatt gtttaaccgc caggtaacgc cgctgcaact ctcagaacaa
    14821 gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagagag caatctggca
    14881 gagctgcgtg gcggcagcaa ttacgcgcaa cgaaaaatca agatagccgc tgcacactct
    14941 ctttccctcg ggctgttacc gtccattatc agccagatgc cgccgctctt tacctgggca
    15001 attgaagcta ttgatgtcga tgaagcggtc gataaactgc gtgaagggca aagtgactgt
    15061 attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat tcgcttattt
    15121 gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact ttttaacctc
    15181 gcgcagccac actttccgtt actgaattac agccgcaact cctacatggg gcgattgatt
    15241 aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt ctcttcgatg
    15301 agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct gccggagtac
    15361 gccatccaac aagaaattcg cagcaggaag ctcgttgtac tgaatcggga cgaactggtg
    15421 atcccgattc aggcctatgc ataccgaatg aatacccgca tgaatcccgt tgccgaacgc
    15481 ttctggcgtg aactgcgcga gctggagatt gtacttagct gacattctcc agcgtcgcac
    15541 tacgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg tcgtggtaca
    15601 gcattatctc aaggctgatc gccagcgtgt gactgtacgc ttctttcaga ttgacctgca
    15661 acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg caataccagc
    15721 tctggagatg tttgtcgctc tggttatgca cttgccactc gggcatgcct gaaacaagta
    15781 atgacggtaa tgccgaaaga ggagaattaa aaagagtgag gagatcgctg cataccgatt
    15841 tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga agtgattggc
    15901 gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg aacaccgggc
    15961 accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac atccatgtca
    16021 gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac cttacttgcc tatgaatatc
    16081 tactgacttt tcaggaaaaa agtcatgatt tgataaaaat catcaaactc aaaaaaaaac
    16141 cgctagtata ttctgaatat tccgaatagt tatgtagatg aaaatacttg tcaccgccgc
    16201 tacttaattc cggtgggaaa ttatttattt ccgcataatc agccatgtat tatttgaaac
    16261 aattagtttg taaataatat aaattgataa catatcaaat acatcaaaaa taccattcac
    16321 aggcattatt aatatagata tcaatatagc aacatcaaat tccaattagt aataagatat
    16381 gctttttaat tgcatgcaaa taattttaaa tgagcgtttt ttgatacttt agcgtgtttc
    16441 ctacccttta atatcaccat gtagagttga tatcactaca acagaaacat gcatgtcatt
    16501 tgtttacttc ttttttacta cagaaaaatt tatctatttt tctaaacatc agtgaaatac
    16561 tcataaaagg taactattat gtctatgcca ttaagcaatg cccttcagag tcaaattatt
    16621 acagataatc acttccttca tcatccaaaa gtcgacagtg agttaatacg taaatatgaa
    16681 cgtgcacgcc tggatacgga aaatatctat cttctgcctc ttgttcgtgg caataaccat
    16741 aattacgatg gaaaaagcgt tgtcgaaatt agaaagcttg atattagtaa agagtactgg
    16801 ccatttaatt atgtgacaga agcatgcaga gaatccgatg gtattacaac aacaggacgt
    16861 atgctctatc gaaacttgaa aatcacctca gctttagacg aaatatatgg tggtatatgt
    16921 aaaaaggcgc atgctgctac agaattagca gaaggtttac ggctaaattt atttatgaag
    16981 agccctttcg atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc
    17041 aatgttccag gttacgccgg aaccacgata ggatatattt caacccttcc agtttctcag
    17101 gcaaaaagat ggaccaatga gcagccaaga attgatatct atattgatca gataataact
    17161 gtctcagggg tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt
    17221 ggaatgggaa atgacccaat aattggtata gaagcctatc ccggtaccgc ggagatccat
    17281 gcaaaaatgg gatataacgt tatccccggt gatgaagatg cgccactcaa acgcatgact
    17341 ctgcaaccgt catctcttcc agaactgttt gaattaaaaa atggtgaatg gaattatatt
    17401 ggtaaatagc cactgagaag aatgagtact aatattcagg ttatacagat atttaaacaa
    17461 taccctgcaa tttacaaagt aatactcatt gcgacttctt aatttaagaa agcgcaagga
    17521 acgtttaccc ttgcgcttat tataataagt caggcgtcga ctttatccag cggccatgaa
    17581 tcaaacagat agccgtcaaa ataaatatcg tcaacgttcg agaattccag cagacgttgc
    17641 ttaacgtttt ccagatgctg ccacattgcc agcttcgccg ctcgggcatc ttttttgatt
    17701 aacgcggcga ggatctgttt gtgatcgccc aaccactctt tgcgatacag gctgtcatcc
    17761 agatggctgt gcaactggat ccacattggg ttgttttccc gccactgcca ggactgacgg
    17821 aacagctcca ccagcatgct gttatgcgtt gcttcggcaa tagcgagatg gaactgcatg
    17881 tcaccgcttt cgctgctgcc tggcgcactg gaagccagtt cacgctcttc cagttgcaat
    17941 gcctgacgca ttttgacgat atcttcgcgg gtagcctgca aagcggcaaa ctcggcgatg
    18001 ttgctctcca ataactgccg cgcctgtaac agctcaaaag gaccggcatc gttgcagacg
    18061 ttggcatccg gactgtctgt gttctggctg cctgagttat caagaacata gataccggca
    18121 ccccggcgta cttccaccag ccctttgatc tccagcatga tcagcgcttc acgtaccacc
    18181 gtccgcgtga catcaagcat ttctgcaatt tcacgctccg gcggcagccg ttcgccaggg
    18241 ttgtacggcg tctttatgat cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt
    18301 tgcgcagagg tggctgattt catggtgttg tgtccgttag gtgcggttaa tcaaattttt
    18361 gccggatgcg gcgtgaacgc cttatccggc ctacaacggg caattttacg gcccccgtta
    18421 atctcaaact ataccattag cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa
    18481 actggtcaac caaatatcag tttgattgac cagtaagtct gtagggtaat cagttagtta
    18541 cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac
    18601 tgcgccaaca aagtcggcat tctgcggcag atcatcggca aaaataccgc tcaggcccag
    18661 cagcgctttc acgcggtctg cgccctgata ctgcgcgttg atcttctgga actccgccag
    18721 catcgggtcg accacgtcaa tggcattacc ctgctcatcc acgccctggg tgtaacgcat
    18781 ccagccagcc acgcccagcg ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg
    18841 caccgggtcc agcagacgct gcggtaactt ctggctgccg tccatcgcaa tctgccaggt
    18901 acggtgacgc agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag
    18961 gtccgtacct tccggcatcg acagcgttgg cgcttgttcc tgcatcatca gggagaaggc
    19021 cgctttgcga taatccgggt tagtcatggt gtcggcaata gtttcatagc cgccgaggta
    19081 accgaggtat gccaggaaag agtggctgcc gttcagcata cgcagcttca tcatttcgaa
    19141 cggcacaacg tctgcaacga actgtgcgcc cactttatcc cagtccggac gaccattaac
    19201 gaagttatct tcaatcaccc actgacggaa cggttcgcag gcaatggcgc acgggtcgta
    19261 aacacccagc tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg
    19321 gtcaaccatt gtgcacggga aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc
    19381 acgcgcctga gccagcccca gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt
    19441 atcgcaggac atcaccgtaa acgctttcaa ccctttttca cgacgcagac gcaaggcttc
    19501 gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt tctagatcgt gcttgatcag
    19561 cgggttattg agatccagtt gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac
    19621 cgttagagag acaatcgccg tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc
    19681 gtcgatttcc ggatgcagcg cttctttcat cgaaccgata attttcagct cggtgctttc
    19741 tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac
    19801 gcggtcgttg cctggcatca ggttaacttc gcagatgccc cagtcgctgt cggtgctttc
    19861 cagcagatga tgggtataca gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg
    19921 cacaatgcgt gattccagac gagaatgatc ccatgacggg cgggcaaccg gcagattgct
    19981 gtcaacaata gtagtcatcg taaactccag aatccgtctt cagagagtga cggcaaggaa
    20041 gaaggaaccg gcacggcgcg cagccatgcc ggtggatatt aacggctaaa gaaagcgcgc
    20101 tggatcgcca gttcgacacc gcgaacttcg gccaggcctt tcagacgacc aattgcggag
    20161 taacctgggt tggttttctt cttcaggtcg tccagcatct gatgaccgtg gtccggacgc
    20221 atcgggatca ggtcttcttt gccttccgct ttacgacggt gttcttcttc aacaatcgct
    20281 ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc
    20341 gggttatctt cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc
    20401 ttgatcatat caaccagatc gttgtcagca cgcacgccgt aggaaccggt gcacatggtg
    20461 aaaccgttcg ccatgctgtt tacggtatca accatccact gcatatcttc aatggtggaa
    20521 acaatgcgcg gcaggccgag gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc
    20581 acgccaactt cttcagcaac tggaataatc gctttcagga agacggcaaa gttttcgcgc
    20641 agcttcgctt tgtcgatatc tttgtacagc tccaggtgtt tacggaactg gtcgagggtg
    20701 tagccttctt ccgcgcccgg aagaccagca atgatgttac gggtcagacg cgctttgtct
    20761 tcgtcgctca tagtggcgaa gcgaacagca gcctgagcaa tttcttcttc ggtgtaatcc
    20821 gcttccgcgc ctggacgttt caggatatgc atttcgaatg cagcgaattc gatctggtcg
    20881 aagcgcagcg ctttggagcc gtctggcagc acgtattcga ggtcagtacg ggtccagtcg
    20941 agcaccggca tgaagttgta gcacacggtg cgaatgccgc actgcgccag gttgcgcaga
    21001 gtctgctgat agttagcgat ccactgctca tagttgccag tgtgggtttt gatatcttcg
    21061 tgaattggta cgctttctac gacagaccac accaggcctg cgtcttcaac gatcgccttg
    21121 cgtttgagga tctcttctac ggaccatact tcgccgttcg ggatatggtg cagcgcggta
    21181 accacgccag ttgcgcccgc ctgacggaca tcagctaaag aaaccggatc gtttgggccg
    21241 taccagcgcc aggtctgttc catgattttt cctctttcac atagtgggtt ccacagcaaa
    21301 gagacgcgga accctggagt tgtttaggta tccggatgaa caaccagacc gcagccgtgg
    21361 cgttgcatcg gatgagatgg cgtataagtt ctaccttttg gtaagacatg tcaacccgaa
    21421 ttctgaaatt ggttaaccac accacaagaa tttcactctc ctggttgttg ctgcaaattg
    21481 cagcaaaagg ttatcgatgg ttattgatca gaggatgtga cattcatcgc aacaatggtt
    21541 gaccaattta cataacatgt cgcccaaaat aacaacggtt caaccaggta aggtaaaacg
    21601 ttatgcatgt gcttaacatt ctctgggtag tattcggtat cggcctgatg ctggtactga
    21661 atttgaagtt caaaatcaat tcaatggtgg ctttgttggt ggcggcgctg tccgtcggga
    21721 tgctggcggg catggatttg atgtcgctgc tgcacactat gaaagcgggc ttcggcaaca
    21781 cgctggggga actggctatc atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg
    21841 actccggcgc ggctcaccag atagcgcata cgctgctggc gcgtctcggt ctgcgctatg
    21901 tacagctgtc ggtgattatc atcggcttga ttttcggtct ggcgatgttc tatgaagtgg
    21961 cctttatcat gttagcgccg ctggttattg ttattgccgc cgaagctaaa attccgttcc
    22021 tgaaactggc gatcccggca gtagcagctg ccactaccgc acattcactg ttcccaccgc
    22081 agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga tatggggatg gtttatatct
    22141 atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg tctgatcctg ccgaagttcc
    22201 tcggcaatct tgagcgccca acgccatcat tcctgaaagc agatcaaccg gtagatatga
    22261 ataatctgcc ctctttcggc gtttcgattc tggtgccgct gatcccggcg atcattatga
    22321 tctctaccac catcgccaat atctggctgg taaaagatac ccctgcctgg gaagtggtta
    22381 actttatcgg ttcctcgccg attgcaatgt ttattgcgat ggtggttgca ttcgtactct
    22441 ttggcaccgc acgtggtcat gacatgcagt gggtgatgaa cgcttttgaa agcgcggtga
    22501 agagtattgc aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag cagaccatca
    22561 tcgacaccgg cattggcgac accatcggca tgttgatgtc ccacggcaat atctcgccct
    22621 acatcatggc atggctgatc actgtgctaa ttcgtctggc gacgggtcag ggtgtcgttt
    22681 cggcgatgac cgccgccggg attatcagtg ctgcaatcct agatccagca actggtcagc
    22741 tggttggcgt gaatccggcg ctgctggtgc tggcgacggc tgcgggttcc aacaccctca
    22801 cccacattaa cgatgcatct ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag
    22861 acacgttgaa aacctggggt ctgctggagc tggtcaactc cgtggttggg ctgattattg
    22921 tgctgattat tagcgtggta gcgtaaaaga acaccagagc ctgccattgg caggctctga
    22981 ctgatgacaa agtctaaaaa tgtgcccggc cagtcccctc gcccctccgg ggagagggtt
    23041 agggtgaggg gaacagacca gcaaagtgcg ctttgtttat gccagatgcg acgttgacgc
    23101 gtcttatctg gcctacaaag ggctaacgtg caggttttgt aggtctgata gcgtagcgcc
    23161 tcaggtacca gcattagcaa tgccctgtga tttctttatt gataaacaaa agtcacgcca
    23221 ataatcgatt gcacattccc tgcagtcacc tgccctccgg tacgtgcata atttgccgtt
    23281 aatcccagac ttaccgccga agtccctact gctcctaacg ataccgtgtt attcgctgga
    23341 ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gtgcaggtga aaacgacgcg
    23401 gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta ataccccagg
    23461 ttttggcttt tcgcacaata aacggtaaga ggaatcggca ctgaaccagg gtagtccggc
    23521 agagtaacgg tgacatcacg agcagaaaca tcacagccgc cagtaggcac caccacatca
    23581 ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt gttggtctgt
    23641 cgcaaaataa gcacggcaat taatgagcca gctttaattg ccaccccgcc cgcactgctc
    23701 acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacg
    23761 cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt tacggtcccg
    23821 gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac atagtctgta
    23881 atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac gaccaggttt
    23941 tgccccacat tcacggcagg cgcaaggttt acataaacat tagcgctgcc accgccaata
    24001 gggatagctg taccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc
    24061 atcagcagta cagcaaacag ggtaataact cgtttcatta caatcatctc ttcgggttca
    24121 gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt
    24181 tactgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg
    24241 aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta gctctaactg
    24301 gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg ccccgctgaa
    24361 gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg
    24421 ccaggccgat gccgccccgg cagacatcag actgaaagaa taaagatcgc cgagatcaac
    24481 cgtggcattg gtggtggaaa ctgtgcacgg tttggcgacg accttaccgt tcaccgtgat
    24541 ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg ccaatacata
    24601 cccacgtttg caccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc
    24661 cgtggcattg atatgccctg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa
    24721 attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaagcgcgg acgatggagc
    24781 attaaggggt atttgatttt gctgctcatt cagaagctgt attcccagtc ccgaagccgc
    24841 tgacaccgta ttttcaagtg caagcagatt ggcattgtgg ctatctgcaa cgccggtaaa
    24901 cccaactttt acggcagaaa cggcattacc acagggtgac agcaaaatac gaaatggaac
    24961 aacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac
    25021 agtaaaattg gttgattcag cggccacact acagccgtta tccctgacat agccgcggat
    25081 agtaatcgtg ctatccgcag ccaaagcgtg actcaccgcc agccacaaaa aagcgcacag
    25141 aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt
    25201 taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt gagcattttc
    25261 ctcttctccc catttcacct gaacttttcc cg
//
LOCUS       DAZUFB010000063        25159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZUFB010000063 DAZUFB010000000
VERSION     DAZUFB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.063"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..134)
                     /locus_tag="ACHY3M_002952"
     CDS             complement(<1..134)
                     /locus_tag="ACHY3M_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFS4317831.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     A"
     gene            complement(127..273)
                     /locus_tag="ACHY3M_002953"
     CDS             complement(127..273)
                     /locus_tag="ACHY3M_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFS4317832.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPCRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(308..586)
                     /locus_tag="ACHY3M_002954"
     CDS             complement(308..586)
                     /locus_tag="ACHY3M_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFS4317833.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARILTKEKAPKNGEFFEA"
     gene            complement(695..889)
                     /locus_tag="ACHY3M_002955"
     CDS             complement(695..889)
                     /locus_tag="ACHY3M_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4317834.1"
                     /translation="MIRMTLADYAKIHGQAKAASDFGVIQCAISKAILAGRNIMVTVK
                     PDGSVIGEEVRPFPSNKKNK"
     gene            996..1712
                     /locus_tag="ACHY3M_002956"
     CDS             996..1712
                     /locus_tag="ACHY3M_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4317835.1"
                     /translation="MENKKSLTTEQLEDAKRLKALYESKKKELGITQYSIADELGITQ
                     GAVGHYLNGRNALNVEVASGFARLLQVSIADFSQSIAAKVAEQAESLKSDANVRYAGE
                     YRAGKRYPVLSSIQAGSWCEACEPYTIKDIDVWLESDAHIQGNAFWLKVEGDSMTAPV
                     GLSIPEGTFVLFDTGREAINGSLVIAKLSDSNEATFKKLIIDGGNKYLKGLNPAWPLV
                     PINGNCKIIGVAIETKLRLV"
     gene            1730..2098
                     /locus_tag="ACHY3M_002957"
     CDS             1730..2098
                     /locus_tag="ACHY3M_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317836.1"
                     /translation="MVGTAIASFFGMLAISTIYGLAHAFIAKSLSEKISQAWAHRSAR
                     FMILVIIAIQGISAFILYGSSLYLLYQGATFTPYTSDYGTLYDGSEDITVAWIVFGLS
                     MAVSVVADIIKVILVLTFAD"
     gene            2466..2738
                     /locus_tag="ACHY3M_002958"
     CDS             2466..2738
                     /locus_tag="ACHY3M_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317837.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLCCKEEDERSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            2742..2966
                     /locus_tag="ACHY3M_002959"
     CDS             2742..2966
                     /locus_tag="ACHY3M_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317838.1"
                     /translation="MNQTYIPSCLRNLPKQKAKPRKQAIKDAKAEVIDQAIQLLREEL
                     RSGKLEGMMMPYQRGYLSAISKLEVLKSEL"
     gene            3099..3299
                     /locus_tag="ACHY3M_002960"
     CDS             3099..3299
                     /locus_tag="ACHY3M_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFS4317839.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKLNNKAA"
     gene            3365..3835
                     /locus_tag="ACHY3M_002961"
     CDS             3365..3835
                     /locus_tag="ACHY3M_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317840.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTDHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSIPDIEYNFGHNQETENWVFY"
     gene            4030..4200
                     /locus_tag="ACHY3M_002962"
     CDS             4030..4200
                     /locus_tag="ACHY3M_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317841.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRMKSLLE
                     KLWDILKQQGRP"
     gene            4276..4446
                     /locus_tag="ACHY3M_002963"
     CDS             4276..4446
                     /locus_tag="ACHY3M_002963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317842.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            4457..5062
                     /locus_tag="ACHY3M_002964"
     CDS             4457..5062
                     /locus_tag="ACHY3M_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFS4317843.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGNRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            5062..5445
                     /locus_tag="ACHY3M_002965"
     CDS             5062..5445
                     /locus_tag="ACHY3M_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317844.1"
                     /translation="MAHSITVRLNKPAREFQDGENIGFNIRAGVQYYDRQTKKKEWTN
                     YNAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
     gene            5469..5762
                     /locus_tag="ACHY3M_002966"
     CDS             5469..5762
                     /locus_tag="ACHY3M_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFS4317845.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCVWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISNN"
     gene            5773..5940
                     /locus_tag="ACHY3M_002967"
     CDS             5773..5940
                     /locus_tag="ACHY3M_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFS4317846.1"
                     /translation="MRGLAYNPSILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRKGGKQ"
     gene            5937..6236
                     /locus_tag="ACHY3M_002968"
     CDS             5937..6236
                     /locus_tag="ACHY3M_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317847.1"
                     /translation="MSVCLIDKRRRGQQIPSVEMPNHTWFCVLDIDGMDTLVDTRHYC
                     DTATATPAKAKKIAALIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            6238..6792
                     /locus_tag="ACHY3M_002969"
     CDS             6238..6792
                     /locus_tag="ACHY3M_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4317848.1"
                     /translation="MNKIDYQALRAKAEKATCGEWSLEYGEERFDAGDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAFNPKVALALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELEAEKQRAKVLFMENARLKSGIAGLIHLGIRYADVEVMKIAGDA
                     QLSTPCTDSIINSIATGIRIKGGE"
     gene            6792..6968
                     /locus_tag="ACHY3M_002970"
     CDS             6792..6968
                     /locus_tag="ACHY3M_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317849.1"
                     /translation="MRVACIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     SARRAAKKRRRAKR"
     gene            6965..7153
                     /locus_tag="ACHY3M_002971"
     CDS             6965..7153
                     /locus_tag="ACHY3M_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317850.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRVGLMKHYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            7155..7364
                     /locus_tag="ACHY3M_002972"
     CDS             7155..7364
                     /locus_tag="ACHY3M_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317851.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            7361..8163
                     /locus_tag="ACHY3M_002973"
                     /pseudo
     CDS             7361..8163
                     /locus_tag="ACHY3M_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8156..8440
                     /locus_tag="ACHY3M_002974"
     CDS             8156..8440
                     /locus_tag="ACHY3M_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4317852.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAINVNIGNE"
     gene            8512..8811
                     /locus_tag="ACHY3M_002975"
     CDS             8512..8811
                     /locus_tag="ACHY3M_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317853.1"
                     /translation="MVCSTFNHLTLQKYQPDPEDLCSLCGGNHGKAAMIECNDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            8893..9084
                     /locus_tag="ACHY3M_002976"
     CDS             8893..9084
                     /locus_tag="ACHY3M_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4317854.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(9065..10243)
                     /locus_tag="ACHY3M_002977"
     CDS             complement(9065..10243)
                     /locus_tag="ACHY3M_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4317855.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            10425..11849
                     /gene="sbcB"
                     /locus_tag="ACHY3M_002978"
     CDS             10425..11849
                     /gene="sbcB"
                     /locus_tag="ACHY3M_002978"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFS4317856.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(11892..12119)
                     /gene="tsuB"
                     /locus_tag="ACHY3M_002979"
     CDS             complement(11892..12119)
                     /gene="tsuB"
                     /locus_tag="ACHY3M_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFS4317857.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12133..13191)
                     /gene="tsuA"
                     /locus_tag="ACHY3M_002980"
     CDS             complement(12133..13191)
                     /gene="tsuA"
                     /locus_tag="ACHY3M_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFS4317858.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(13370..14728)
                     /gene="plaP"
                     /locus_tag="ACHY3M_002981"
     CDS             complement(13370..14728)
                     /gene="plaP"
                     /locus_tag="ACHY3M_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFS4317859.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(14718..14780)
                     /gene="yoeI"
                     /locus_tag="ACHY3M_002982"
     CDS             complement(14718..14780)
                     /gene="yoeI"
                     /locus_tag="ACHY3M_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFS4317860.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(14995..15924)
                     /gene="yeeY"
                     /locus_tag="ACHY3M_002983"
     CDS             complement(14995..15924)
                     /gene="yeeY"
                     /locus_tag="ACHY3M_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4317861.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEDWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAMEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(15970..16794)
                     /gene="yeeZ"
                     /locus_tag="ACHY3M_002984"
     CDS             complement(15970..16794)
                     /gene="yeeZ"
                     /locus_tag="ACHY3M_002984"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4317862.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTSRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(16877..17131)
                     /gene="yoeB"
                     /locus_tag="ACHY3M_002985"
     CDS             complement(16877..17131)
                     /gene="yoeB"
                     /locus_tag="ACHY3M_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFS4317863.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(17128..17379)
                     /gene="yefM"
                     /locus_tag="ACHY3M_002986"
     CDS             complement(17128..17379)
                     /gene="yefM"
                     /locus_tag="ACHY3M_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFS4317864.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            17663..17713
                     /gene="hisL"
                     /locus_tag="ACHY3M_002987"
     CDS             17663..17713
                     /gene="hisL"
                     /locus_tag="ACHY3M_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFS4317865.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    17689..17812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            17859..18758
                     /gene="hisG"
                     /locus_tag="ACHY3M_002988"
     CDS             17859..18758
                     /gene="hisG"
                     /locus_tag="ACHY3M_002988"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFS4317866.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            18764..20068
                     /gene="hisD"
                     /locus_tag="ACHY3M_002989"
     CDS             18764..20068
                     /gene="hisD"
                     /locus_tag="ACHY3M_002989"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFS4317867.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASENITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELTPQGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            20065..21144
                     /gene="hisC"
                     /locus_tag="ACHY3M_002990"
     CDS             20065..21144
                     /gene="hisC"
                     /locus_tag="ACHY3M_002990"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFS4317868.1"
                     /translation="MSTDNFLSVTDLARKNVRELTPYQSARRLGGNGDVWLNANEYPT
                     AVEFQLTQQTLNRYPECQPKAVIENYAQYADVKPEQVLVSRGADEGIELLVRAFCEPG
                     KDAILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGIADKLDGVKVVYVCSPNN
                     PTGQLINPQDLRSLLDMTQGKAIVVADEAYIEFCPQATLAGWLSEYPHLVILRTLSKA
                     FALAGLRCGFTLANEEVINLLLKVIAPYPLSTPVADIAAQALTPQGISAMRKRVEQIL
                     EERQYLVDNLKTIRCVEQVFDSETNYVLARFTASSSVFKSLWDQGIILRDQNKQPSLS
                     GCLRITVGTREESQRVTDALRAEQV"
     gene            21141..22208
                     /gene="hisB"
                     /locus_tag="ACHY3M_002991"
     CDS             21141..22208
                     /gene="hisB"
                     /locus_tag="ACHY3M_002991"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFS4317869.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPEVVPVLLKLQ
                     KAGFKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVTFDEVLICPHLPADECD
                     CRKPKVKLVEQYLVEQAMDMANSYVIGDRATDIQLAENMGISGLRYHRDTLNWAMIGE
                     QLTKRDRYAHVERNTKETQIDVKVWLDREGRSKINTGVGFFDHMLDQIATHGGFRMEI
                     AVKGDLYIDDHHTVEDTGLALGEALKLALGDKRGINRFGFVLPMDECLARCALDISGR
                     PHLEYRAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            22208..22798
                     /gene="hisH"
                     /locus_tag="ACHY3M_002992"
     CDS             22208..22798
                     /gene="hisH"
                     /locus_tag="ACHY3M_002992"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFS4317870.1"
                     /translation="MNVVILDTGCANLHSVKSAVARHGYDPVVSRDPDVVLRADKLFL
                     PGVGTAQAAMDQLHERELIELIKACTQPVLGICLGMQLLGRRSEESNGVSLLGIIEQD
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM"
     gene            22798..23535
                     /gene="hisA"
                     /locus_tag="ACHY3M_002993"
     CDS             22798..23535
                     /gene="hisA"
                     /locus_tag="ACHY3M_002993"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFS4317871.1"
                     /translation="MIIPALDLIDGTVVRLHQGNYAKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAQRQIPQIKTLVAGVNVPVQVGGGVRTEADVAALLDAGVARVVVG
                     STAVKSPEIVKSWFARFGADALVLALDVRIDEHGNKQVAVSGWQEDSGVSLEELVETY
                     LPVGLKHVLCTDISRDGTLAGSNVALYEEVCAKYPQVAFQSSGGIGGIEDVAALRGTG
                     VRGVIVGRALLEGKFTVEEAIQCWQNA"
     gene            23517..24293
                     /gene="hisF"
                     /locus_tag="ACHY3M_002994"
     CDS             23517..24293
                     /gene="hisF"
                     /locus_tag="ACHY3M_002994"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFS4317872.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSIDDAARILSFGADKISI
                     NSPALAEPELITRLADRFGVQCIVVGIDTWYDADTGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVRDVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            24287..24898
                     /gene="hisIE"
                     /locus_tag="ACHY3M_002995"
     CDS             24287..24898
                     /gene="hisIE"
                     /locus_tag="ACHY3M_002995"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFS4317873.1"
                     /translation="MLTKQQCRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGHFLNVVSIAPDCDNDTLLVLVNPIGPTCHKGTS
                     SCFGDASHQWLFLYQLEQLLAERKTADPASSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(24955..>25159)
                     /locus_tag="ACHY3M_002996"
     CDS             complement(24955..>25159)
                     /locus_tag="ACHY3M_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027959.1"
                     /note="regulator of length of O-antigen component of
                     lipopolysaccharide chains; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFS4317874.1"
                     /translation="LLEVQALEVAPDSVHAYRYVMKPTLPIRRDSPKKAITLVLAVLI
                     GGMIGAGVVLGRNALRGYKAKAE"
BASE COUNT         6778 a         6155 c         6284 g         5942 t
ORIGIN      
        1 gcttctgcgt ccttgtaaaa cggcacatcc ataattttac ggtgcagcaa ggcatacccc
       61 ttaccgctgc tttgatgcgg ttgttgtagc cttctggcct ctctggcttc ggctagatta
      121 gatatgttac tcatgacctt tctccttctg catcagcttc actttttcca actcagcccg
      181 gaatcgacaa ggctgcttga agctggacag gaagcgatca cgtagtatgt gtttgtgaat
      241 tttgtcctgg taaggactga gttgttttgt cataatgact cctgtggatt gatccagtct
      301 ttctacatca ggcctcgaag aattcgccgt tcttcggggc tttttctttt gtcaggattc
      361 tggctacttt cttagccagt tccgccaact cctcgtcttc aacaccccat tcaagaacag
      421 ccagaagcat tcccattttg gggatgaagc tatctttcca tcgcgaaatt tgcgattcat
      481 taatccctaa cgcgtcggca acctttcgct gaccacgtac agcaattcga ttcaggatgt
      541 tgcttgtaat tgcattcgct ttcttgcgag tacttgtaag ttgcatatgt aagtatttcc
      601 ttaacaaata agaagttatg cgcatcaaat tatgcgcgtt gtattcccgc atttcggcgg
      661 gaatgatgac catgactgtt aaagagcggt gttactattt gtttttcttg ttgcttggga
      721 aaggacgaac ttcctctcca atcacactgc catcaggctt taccgtaacc atgatgttac
      781 ggcctgccag aatggccttg ctgatagcgc actggattac accaaagtca ctggctgctt
      841 tagcctgtcc atggattttg gcgtaatcgg caagtgtcat tcgaatcata tgcactctcc
      901 gttattaacc atgaacaaag aatactacag gtattcaaag caatcaatac tcagggtatt
      961 tttagtttaa gtaccttagc tattagaatt aagctatgga aaataaaaaa tcactgacga
     1021 cagaacagct cgaagacgct aagcggctta aggctttgta tgagtcaaaa aagaaagaat
     1081 tgggaataac ccaatactca atcgctgatg aactgggtat cacccaagga gcggtagggc
     1141 attatcttaa tggcagaaac gcgctaaacg ttgaggtcgc atctggtttt gcacgtttgt
     1201 tgcaagtctc aattgctgat tttagccagt caattgctgc caaggttgca gaacaggcag
     1261 aaagccttaa gagcgatgcc aacgtaaggt atgcagggga atacagagca ggaaagaggt
     1321 atccggtgtt aagcagtatc caggctggct cgtggtgtga agcatgcgaa ccatacacca
     1381 ttaaagacat agatgtttgg cttgagtctg acgcgcatat tcaaggtaat gcgttctggc
     1441 ttaaagtgga aggtgattca atgacggcac cggttgggtt aagcattcca gagggaacat
     1501 tcgttctttt cgataccgga agggaggcga tcaacggcag cttggtcata gcaaaacttt
     1561 ctgactctaa cgaagcaaca ttcaagaagc tgataatcga cggcggaaat aaatacctca
     1621 agggacttaa tcctgcatgg cctctcgtgc caatcaatgg aaactgcaag attataggcg
     1681 ttgcaattga gacaaaacta aggctggttt gatcacgcaa ggggcgatta tggttggaac
     1741 cgctatagca agcttttttg ggatgttggc aatctcgaca atttacggct tagcgcatgc
     1801 ttttattgcg aaatctctat cagaaaaaat aagccaggct tgggcgcata gatcagctcg
     1861 tttcatgatt ctggtgatca tagcaataca agggatatct gcatttatcc tctatggatc
     1921 aagcttatac ctattgtatc aaggcgcgac atttacgcct tacaccagtg attacggaac
     1981 tctatacgat ggtagtgaag acatcactgt ggcttggatc gtctttggtt tatctatggc
     2041 cgtgtctgtt gtagcagaca tcattaaggt aattctcgtc ttaaccttcg ctgactaacc
     2101 tataatcccg gcagcaatag ctatcgggat ccacttcaca tatcccgcat aaaaagcact
     2161 gaacaagcag acaccgaaaa aataaatatc ctttgtattc atttgcttat cattatttca
     2221 ccaaaaataa ataccttggg tatttacaca ataaaatacc tacagtattc tttagccatc
     2281 agcagggcgc tggaagccaa acggaacaga ttggcaggct ctttaacatt gatgggattg
     2341 tcccgccgaa atgcgggaac caaagagtag ttggctttgg ggtgacgtga agtgcagctg
     2401 cacgacggca accggaagat aagcacccgg cgcgtcaccg ccaaagtcaa tcatcggagg
     2461 tcaacatgac agtagtcatt acatatctgg ctgacgataa cgccagaaat cgccgcagag
     2521 cacgcagaca ggctcaacgt gaacaggcaa tgcaagagca gcgactggca cgaaaaattg
     2581 cgctaaagct ctctggttgc gtcagagcag ataaagcagc atcactcgga agcctttgct
     2641 gcaaggaaga agatgaacgc agtggaagta tttgcctgcc aaacgtagcc atttacgcgg
     2701 caggataccg gaaatcaaaa caactgacag cgaggtaagt gatgaatcag acatacattc
     2761 catcatgctt gagaaatctg ccaaagcaga aagcaaagcc ccgcaagcaa gccataaagg
     2821 acgctaaggc agaggttatt gatcaagcaa tacaattgct cagggaggag ttaagaagtg
     2881 gcaagctcga aggaatgatg atgccctatc agcgcggata tctatcggcg attagtaagt
     2941 tggaagtatt gaagagtgaa ttatgaacta tctggaattt ccggatggtt cattgttttg
     3001 gcagcaaacc acttatttga ggtgatgtat ggaagaagaa tttgaagagt tcgaagagca
     3061 tcctcaggat gtgatggaac aataccagga ctatccgtat gactacgact attgataaaa
     3121 atcaatggtg tggacaattc aagcgatgca atggatgcaa gctgcaatcg gaatgcatgg
     3181 ttaagcctga agaaatgttt cctgtaatgg aagatgggaa atatgtcgat aaatgggcaa
     3241 tacgaacgac ggcaatgatt gccagagaac ttggtaaact gaacaacaag gctgcctgat
     3301 ggcggccttt atttttggca taaacaacag aataaacaca gcactgaatt atttgaggtg
     3361 agatatgaca aaatcatgga gcgtaccttt tcctgaatca gaaactgaac atgatggaat
     3421 gcctgttttc tggagattcc aagcgacagt tgaagaagat ggaatcaaaa tattcgcact
     3481 tcaatatata gcttttcatc agacagatca ttatgcatgg ttggttcctg cgcattggat
     3541 tgttaatttt aaaccagcac caaatcagtg gttacaggaa tggaaacaaa ggagaaatag
     3601 atatgcaatt aagaaagtag caaaaaatgc cgaaagatct tttgcattcc cgacgaagaa
     3661 acttgccatt gaaagtttat tgcgccggaa gaagtaccat ttaatgagaa tcaaacaaga
     3721 tttggctgtt gtatcaactc ttgttgatgg gatgaagaat attgatacat caataccaga
     3781 tattgaatat aactttggac acaaccaaga aacagaaaat tgggtgtttt attagtacga
     3841 ataagcactg tgtattcatt ccaacgagtg aatacacgga gcaatgtcgc tcgtaactaa
     3901 acaggagccg acttgttctg attattggaa atcttctttg ccctccagtg tgagggcctt
     3961 tttatatgca taccaataac gcttcactcg aggcgttttc gttatgcaat caaacagaag
     4021 gagcatccta tgcaacagtt cgctattgca ggggcggcat cggttcgccc tttcaacccg
     4081 attttatcgg tacagcattc acgaaaaaat attttaaccg gagcagactt taaacaacca
     4141 agaatgaaaa gcttgctcga aaagctttgg gatattttga aacaacaagg ccgcccatga
     4201 gttttacaga taactggtca gacgaagaat tcattcgtca gatgaaagaa ttaatcggta
     4261 acgaaggaga tattcatgtc acttgcaacc accgtgaagg agagcaagtt acagagacgc
     4321 atgtacacgc agcaggcgtt aatgtatcgc cagaagggag atcgtgaagg tgttcgcgta
     4381 tttttaaatg cggcaaagac tgaagtatta aatcagcgtt atttccttgg gccatgtcca
     4441 ttctgagaac aatcatatga gcaaagaatt ttacgcaaga ctggcagcta ttcaggaaaa
     4501 tctgaacgcg cccaagaatc agtacaactc attcggtaaa tataaataca gaagctgcga
     4561 agacattctt gaaggcgtta agccgttact gaatggtctg tttttatcaa tcagcgatga
     4621 agttgtgttg attggtaatc ggtattacgt gaaagccacg gcaactatta ccgatggcga
     4681 aaacagtcat acggcaaccg ctcttgcacg agaggaagaa agcaagaaag gaatggattc
     4741 tgcacaagtt acgggagcta caagctctta tgcacgcaag tattgcctca atggtttgtt
     4801 cggcattgat gatgcgaaag atgcagatac agacgagcat aaacatcagc agaacgcagc
     4861 agcaaagcaa tcaaaaccat cacctacacc tgaacaggtt ctaaaagcat tcactgacgc
     4921 agcattgcag aaaaacaccg tagaagagct taaacaggcg ttcgccaaag cgtggaagat
     4981 gctcgaaggc acaccggagc agcacaaagc gcaggacgtt tacaacatca gacgagacga
     5041 attagaagga gcggctgctt aatggcacat tcgattactg taagactaaa caagcccgca
     5101 agagagtttc aggacgggga aaatatcgga ttcaacatcc gtgctggcgt tcagtattac
     5161 gatcgccaga caaaaaagaa agaatggaca aactacaacg ccgttgtatt tgccaagccg
     5221 ggagcgcaag cggattacta ccgtagtgtt cttgttgaag gtggcattgt agaaattacc
     5281 ggagaaaaca tcagggttga tgtttatcag gggcaaaatg gtcaatcaat cactcttgaa
     5341 ttactgaatg caaagattgg atttgcagct tcaggaaatg gcccgcagca gcaaagtagt
     5401 aaccagcaga acacccctgt atacgacgat tccatcccat tctgatttag aaaaataagg
     5461 atttaattat gccagcgcct ctgtatggtg cggatgaccc gcgccgctgt tccggcaatt
     5521 ccgtatcgga ggtgctggat aaattcagga aaaattacga ccggataatg tcgctaccgc
     5581 aggaaacaaa agaggaaaag gaatttcgcc actgcgtatg gcttgcagag aaagaagaac
     5641 gcgagcgaat ttaccagaca tcaatccgac cattccgcaa agccacatat acccacttcc
     5701 ctgaaattga cccgcgcctg cgtaattacc gctcacgcta tggcgctatc agtaataact
     5761 gaggaattta ccatgagagg acttgcatac aatcccagca ttcttccggc agaaatgatt
     5821 attcgccagc gcgtaaagcc aatgccatcg agagaggaat tacttaagag aaattctttt
     5881 ccgtcagtaa atcaaaacaa atatctgaat gcgatgttgc gtaaaggagg caagcagtga
     5941 gtgtatgtct tattgataaa cgtcgacgtg ggcaacaaat accatctgtt gaaatgccga
     6001 atcacacatg gttttgcgta cttgatatcg atggtatgga tacgttggtt gacactcgtc
     6061 attactgcga taccgcaaca gctactccgg cgaaagcaaa gaaaattgct gctctgatag
     6121 aaaactggac tccacctgat ggttggtgca atgggaatga tcgagattgg catgaaaaaa
     6181 tgaagggcta tatctgcgat ttcttacgta aatgcaacgg attcagggtg atgtgacatg
     6241 aacaagattg actatcaggc actgcgtgcc aaggcagaaa aagcaacgtg tggtgagtgg
     6301 tcgctcgaat atggagagga gagatttgat gctggtgatg cgctaattca tcgtgaagtt
     6361 gttggatatc ttcccatttg cagaattgaa ggagcgcatc ctgaaagcgg tttcgatgaa
     6421 gatttccaga tggaacagca ggccaatgct gaatttattg ctgcctttaa tccaaaggtt
     6481 gctttggcac tgctggatga acgggaaaga aaccagcaat acatcaaacg ccgcgaccag
     6541 gagaacgagg atattgcgct aacggtaggg aagctgcgcg ttgagcttga agcagaaaaa
     6601 cagcgggcaa aagttctatt tatggaaaat gctcggctta agtcaggcat agccggtctg
     6661 atacacctcg gtattcgata tgcagatgtt gaggtcatga aaattgctgg agatgcccag
     6721 ctttctaccc catgcactga cagcatcata aacagcattg caacaggcat tcgcatcaaa
     6781 ggaggtgagt aatgcgtgtg gcatgtatcg gcttgttacc gtacccgact cgtttttggg
     6841 cttctgcgct aattgcaaag ccgcatgtcc tgatggctga caacatcatc ccggcaccaa
     6901 agcgccgcca taccggtatt gcatcggcac gacgagcagc aaagaaacgc aggagagcaa
     6961 aacgatgaaa aaccgtaaag caaagattct gttagttcgt agaaacgctc ctggcgtctg
     7021 gcagtgggtg agactcagca accgacgggt ggggttaatg aaacattacg ggatgatgga
     7081 ttgtggtttt tgcaaaaagc ccagcgcggc gcaaaaccgc tggaaaaacc acttgcgtac
     7141 taaaggagag tgatatggct attgccgcaa gttacaccat gcatctctat tgtgactgcc
     7201 gccagtgtac ggaaggtgta tatccagtgc cagacttcgg agagtatatc ggcacgtcat
     7261 gggctggttg tgcaaaagag gcgcgtaagg atggctggcg aataagcaaa gacaaaacac
     7321 gtgcttttgc gcctgggcat aaagttttga ggattaacaa atgaccacta ttaccagaga
     7381 acaagctctg aaaattattg aggcagccga tgaggttatt agtgcgcttg ccggaactaa
     7441 cgaggatgtt caccctggta gcgataacat gctacgcctg tgggatgacc tgaatgaccg
     7501 ttacgcgccg ccggaagttg tgcgtgagct ggcacggatt gcgctggcat cgttggaagc
     7561 aaaaccaata ggtgcttcca cattgcagaa cagcaagttg acggcacaag tgactacctc
     7621 aaggatggag aatggcctat tgataatggg acaattgaag tatacgccgc ccctccagtg
     7681 ccagtagtac ctgaagaaaa accaatgcct aaccctctta gcatgtacgc ggtcgatgct
     7741 gttgccgcta ttgcagaggt gagaggctgg aacgcctgcc gcgccgccat gcttcatggt
     7801 gccgaacctg taagccaaac ttacaagtca caacacacgc agtttgaaca agttgctgac
     7861 ctctacgaaa tgcaatttga tgacggtcgc acttgtgcct ttcacactga tgcgcaaaag
     7921 gctgcgcaat ggcttcaggc atgcgacgga aacagggttc aggaatacgt gaagctggag
     7981 cgattgcgta atgcgctatc gggcaactct ccggtaactc cggatggttg ggttatggtg
     8041 ccgaaggaac caactcaggc catgattaaa gcgtggctat cagagattgc taactttcgc
     8101 ggtcatgctg ctggttacag ggctgctctt gaggcagcac cacaggaggt taaccgtggc
     8161 taacctgcaa cttgccgtca aaggtgaata cttcgatgcc atgattcgcg gagagaaaac
     8221 agaagagtat cgcctgtgta atgactactg gaataagcga attatgttcc gggagtatga
     8281 ccgcctgatt atcacaaagg gatatccgaa gcgcgacgac tccagccgca gaatagacgt
     8341 cccgtatgac ggatatgaaa tcaagacaat cacacatccc cacttcggcg ataaaccggt
     8401 aaaggtgttc gcgataaatg tgaatatcgg caatgaataa caaccctcgc atttgcgggg
     8461 atttctttta tctgaactcg ctacggcgag ttttgtttta tggagatgat tatggtctgt
     8521 tcaacattca accatctaac gttacagaaa taccagccag accctgaaga tttatgctca
     8581 ctgtgtggcg gaaatcatgg taaagccgcc atgatcgaat gtaatgacaa aatccacatt
     8641 tgccttaatt gcgttgatgt cctcgttgat atcaaaaatg agagagaaga taaaaagcgt
     8701 agcgaggcta ttcgcgcctt agattcatgg atgcgagatg ggtatagtgc tgcgcaaatt
     8761 tatgacttag caatatcaaa aggcgaaata ccaggagtac gcatcgaata agacataacc
     8821 gatattcgaa ttgaagaact gaaagaacac caagccgcct gatggcggtt tctttttgcc
     8881 tggagaatta agatgaccga taccagcctg attcctgaga aagaagtgat gaacaagctc
     8941 ggtgtttcat cacgtcagac aatctggaac tataccaaac gacacggatt tccgaagcca
     9001 gtcagaaccc accccaaatc ataccttcgt gaagctgttg aagggtggat tcttaacggt
     9061 ggcgttaatc agaaatgctc ctgatgtgcc agaacaattt atcagcgtga agctcatacg
     9121 ctttgcgctg agcctctatc caatcgtgtt tattgtatac ggacattatc ccgccaagtt
     9181 catgaccgag cattcgctca gtaacatgag gctcgacatt catctcggac agacgtgtaa
     9241 caagcgttct tctgaaatca tgtgttcgcc agtaacctag atccatccct ccccttattc
     9301 tatttacgaa tcggttggct gcagcgatgc tgatcggttt gtttatgtct tctccaggga
     9361 aaagaacatc attgtacgtt gtcatggcct tttcgagaaa aggcttaatt tgttcgaaga
     9421 ttggacgacg gataacatta cccatcttgc tatgctcttt cggtacggtc catacgttgt
     9481 ccagcaggtc aaattctgtc tttgttgcca gcctaagctc tgagagcctc gccccccaca
     9541 gcataagcat ctgatgaagg agcttatttg acgtagacgc acggcttctt tcaatagcaa
     9601 gccaaatctt agccagttcg tgatacgaca gtacccgatc ccctacctca gcgcgggagc
     9661 cgaagtccct tggctggatg ctcataattg cgcaactatc tatcaactga cgccgcatgc
     9721 accaactaat tgctgatctt agttgactta gcacctgcct tgctcggcgt ggattatctt
     9781 tttcttcttc ggtaagcagg tcaacccatt gcttaaccgt gatagaagat gccggacgat
     9841 taggaaaggc gtcatgcatg cgcttcataa ccgttgatcg gtaaagtgcc tgggtctttt
     9901 ctctgagagt tgtagagacg tagttgtcga accagtagtc gagacactgg gcgaccgtca
     9961 tggagttctc caccttctct tcaaaataag tgcgtggatc cgttcctgag aaatagagct
    10021 ttcgcaagtc agcagtgatc tgtctggcat ccttcaaaga cagggatggg tatcgaccaa
    10081 gcccaagtcg attaggcttg ccatgccagc gatagcggta ctggaactgg atgaccccct
    10141 tcggtgaaat tcgtacgctg aggccatcgg catcagccac ttcttgtggg cccgaatatg
    10201 gtttaccata aatagtacgc agttttgtgt cgcttatagc cataaataat atttggtacg
    10261 ctcaaaaata atggtttggt acacatctgg tacacaatat cacatggaca acagcgcaca
    10321 gtaaacaact atatttgacg ttcgtagaca tactcaggtg aaaaaatggt tgtttttcga
    10381 gttataacag acagttaatt aactacagtg gacaatgcta gacaatgaca gacacagata
    10441 aacaacctac cttcctcttt cacgattacg aaacctttgg aacgcacccc gcgttagatc
    10501 gccctgcaca gttcgcagcc attcgcactg ataacgaatt caatgtcatc ggcgaacccg
    10561 aagtctttta ctgcaagccc gcggatgact atttacccca gcctggagca gtattaatta
    10621 ccggtattac cccgcaggaa gcacgggcga aaggagaaaa cgaagccacg tttgccgccc
    10681 gtattcactc gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt
    10741 tcgacgacga agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga
    10801 gctggcagca tgataactcg cgctgggatt tactggatgt tatgcgtgcc tgttatgccc
    10861 tgcgcccgga aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg
    10921 agcatttaac caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg
    10981 tgtacgccac tattgcgatg gcgaaactgg taaaaacgcg tcagccacgc ctgtttgatt
    11041 atctctttac ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga
    11101 aacccctggt tcacgtttcc ggaatgtttg gggcatggcg cggcaatacc agctgggtgg
    11161 caccgctggc gtggcatcca gaaaatcgca atgccgtaat tatggtggat ttggcaggag
    11221 acatttcgcc attactggaa ctggatagcg acacattgcg cgagcgttta tataccgcaa
    11281 aaactgatct tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc
    11341 cggtgctggc ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc
    11401 gtcagcattg cctcgataac ctaaaaattc tgcgtgaaaa tccgcaagtg cgcgaaaaag
    11461 tggtggcgat attcgcggaa gccgaaccgt ttacgccttc agataacgtg gatgcacagc
    11521 tttataacgg ctttttcagt gacgccgatc gcgcagcaat gaaaattgtg ctggaaaccg
    11581 agccacgtaa tttaccggca ctggatatca cttttgttga taaacgaatt gaaaaactgt
    11641 tgttcaatta tcgggcgcgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct
    11701 ggctggagca ccgccgccag gtattcacac ccgagttttt acaaggctat gcagaagaga
    11761 ttcagatgct ggcgcagcag tatgccgacg ataaagaaaa agtggcgctg ttaaaagcgc
    11821 tttggcagta cgcggaagag atcgtctagt ttgaagcctc cgccgctgat acggcggagg
    11881 acaatgttgg gtcaggcttt ttgaacggtg atgctccacg cagcatcgcc aatctgctga
    11941 taatcggtga tggcatgtcc ttcttctgcc gcccattgtg ggattgcttc tgttgcctgg
    12001 gtgcaatcaa actcaattac caactcatcg ccgctggcca tttctgccag tgctgctttt
    12061 gcttctatga gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc
    12121 tttacacctt acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac
    12181 atacacaagc caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc
    12241 ctgccaggtc atcattgcag tcataaccag tccattaccg atagagcaac cacctgcaat
    12301 actggcaccg aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc
    12361 agctgcgcga acgcggaact cacggctcgc tttggctgca ataaatgacc ccacgaagat
    12421 ccctaacacc aggaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg
    12481 cagaatattg gccgttgggg aagtgattcc taacccaaac atgcgtccgg ttgcttcact
    12541 caggggccag gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca
    12601 gcgtttttca aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt
    12661 gagttttggc ttcttcaact ctttcatcac cacccagagc gttatcacca gcaaaacggc
    12721 aaccaacggc cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtactgta
    12781 gtgctgcaag gtttgattta aaccactggc atgtggagaa cgcatcaccg cactcatcac
    12841 catataagtg aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt
    12901 cccggtggca catccacccg ccagaacaat tcccagcccg aagatatacc cacctataac
    12961 agtaccaagc cacgggaacg ccccggcttc gtaagtcagt aggcccgcct gaattaacgc
    13021 aaaaaccccc acgctttgta ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt
    13081 tttcacgata tacatatcgc gaaaaccacc tgtcaggcaa aaacgcccac gctgcatgac
    13141 aaatcccagc agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt
    13201 aaccagatga atgttaatag ggaaattatt catgactggt ggaatgcaga ccaataacca
    13261 aattctctaa attagaacaa atggttatta atgaggatgt cgataaaaaa gaataaaaaa
    13321 accggagccc aatattcatt cgactccggt ttattattac aaaagatgat tacgctacgt
    13381 cttcgtactg cggaaccggg ttacggaaac tcttggtgac gcaagccagg taaatcagac
    13441 cgatagccgc ccagatcaga cccagaacca ttgagctttc ttccaggtta acccacagcg
    13501 caccaacggt cagcgcacca cacatcggca ggaacagata ctggaagtga tctttcagcg
    13561 tcttgttacg cttctcacgg atccagaact gcgagatgac cgacaggtta acgaaggtga
    13621 acgccaccaa cgcaccaaag ttgatcagcg ctgtagccat taccaggtcg aagttgattg
    13681 ccagcaaggc aatcgcgcca accaggatga tgttcatcgc cggagtacgc catttcgggt
    13741 gtacataacc gaagaagctt ttcgggaaca cgccgtcacg acccattacg tacatcagac
    13801 gcgctacgcc tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg gagaagatca
    13861 gcgcaccaac ctggaatgct ttacctgcaa catacagcat gatttcaggc tgtgacgcat
    13921 ccggatcttt aaagcgagag atatccggga agtacagctg caggaagtaa gttgcaaaga
    13981 tgaagatcat gccgccaatc agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg
    14041 catctttggt ttcttccgac aggttgctga tgccgtcaaa gccggtaaag gagaagcaca
    14101 ggattgtcgc cccggtaatc atcgggataa catgcgcatc gccagaccag aatggacgag
    14161 tgctcgccag cgtaccagcg ccttcacctt caaatacgcc ataaacaacc atgcccagaa
    14221 tcaccgcgat cagcaccacc tgcaacacga cgattacagt gttgaagttc gctacggatt
    14281 tcagactacg caggttaaag gcggtcatga aggccaccaa cgccaccaca aacatccacg
    14341 atgggatgga aggcaccaga gcttcaaaat agattttcgc cagcagaatg ttgatcatcg
    14401 gcgcgaacag atagtcgagc agagaagacc aacccaccat aaagccgaca gtcgggctaa
    14461 tggatttctg ggcgtaagtg tatgcagagc cagcagaagg atagcggcga accagcttcc
    14521 cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat
    14581 gaccatccgt gaggcctgaa acgataccaa aagtatcaaa cagcgtcatc ggctgcatat
    14641 aggcaagacc catcattaca accggaacta acgtaagcgt tttacgcaat tccacgcgag
    14701 aggtgtttgg agtaacgtta tgcgacatgg tcattctcct ttacggtgat aaccgtcgcg
    14761 taagcaaaaa attgccccat ttttttggat tcctcagcga caacaactgt cgatttttag
    14821 taaatatcta tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa
    14881 aataaccgac gctgatgaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc
    14941 ctaaatgggg gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat
    15001 ggctgatttt ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat
    15061 ttcatcccgc caccagccgc tggcaagccc tgcggtctgt tcgttccagc caatccagac
    15121 gggttcaaag agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa
    15181 acgttgcgcc agataacggg gtaaatagcc acagccgagg ccgctaattt gcagctccag
    15241 cttggtttta aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt
    15301 agaagcgcct gcctgcgcgg tatcccccac cacaatcgcc cgatatcgct taataatacg
    15361 acgatttaac ggctcttctt ccatggctaa cggatgatga ggcgcgaccg caaagacttg
    15421 ttccagatcg cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc
    15481 gccaacaata atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc
    15541 attgataaac ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tcagtggcgc
    15601 aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagtcttc
    15661 atgcagtttg atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccctt
    15721 ctctaataac atttttccgg tacgggtgaa tttagcgcgg tgaccgctac gatcaagcaa
    15781 ttgaatatta aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt
    15841 gtagagtttg gccgacgccg ccgcaaaact gccttctttt tctaaggcat cgaggatcat
    15901 caacacatcc agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg
    15961 cgtgatcgtt tactccagcg gcattaccag cggatcggga tactggtatt caaaccccag
    16021 ttcattacaa atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct
    16081 gaactgcgga ggttccagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc
    16141 agggtgagct ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag
    16201 agtgatagcg ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg
    16261 cgcggttttt ccggcaaaga agcgtccggg atgacgtccc ggtccgacca ggcccgcaag
    16321 acgcagaata tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa
    16381 tactcgtcca ctgttggtta ccggattacg cgaggtggtt tctttcaccg tgccttgcgc
    16441 gtcgccatag acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc
    16501 gctatccact aactcttgta ccgcttgtaa atagaactca tcgccggagc cgctacgacg
    16561 tgccggaagc gtaatgacca gtgcatcggc atccatcagg gcatccagat cgtcagaatc
    16621 gcaaactaac tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc
    16681 ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga
    16741 catcgccagc ggcatgccta accaccctaa cccgacaatt gcgacctttt tcatccgtta
    16801 tctcctgata caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt
    16861 ctatgaatga atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcgtcag
    16921 taaccgcgta taccagacgg tgctcctctg taatgcgtcg ggaccagaag cctgacaaat
    16981 tatgcttcag gggttctggc ttccccttac cttcaaatgg tgttctgcgg gtatctttga
    17041 taagttcatt gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat
    17101 cccatgactc ctcagaccag attagtttca ctcaataatg tccttttccg ttcctttgcc
    17161 tgatttcagg ctatcgattg agtccatcaa tctccgggcg ttagcggggg aacgcagtag
    17221 ataagccgtc tcttccagcg agttgtattc ttcgagtgac atcagaacac aagcctctcc
    17281 attctgacga gtaataagga tcggggcatg atcttcaacg gctttcatca ttgttgccga
    17341 caaattctga cgcgcttcgc tgtaactaat tgtacgcatg tcaatctcct cttttgtaca
    17401 gcttattgta caatgataga cgttgattca ctatttatta attagtttgt agatcaagac
    17461 attgtcagtg agacgaaaaa tccaggcttc gctattttgg gtaccgtcag gttagaggac
    17521 agtcctctta gcgccctcct ttccccgctc attcattaaa caaatccatc gccataaaat
    17581 atataaaaaa gcccttgctt tctaacgtga aagtggttta ggttaaaaga catcagttga
    17641 ataaacattc acagagactt ttatgacacg cgttcaattt aaacaccacc atcatcacca
    17701 tcatcctgac tagtctttca ggcgatgtgt gctggaagac attcagatct tccagtggtg
    17761 catgaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt attgcgcggt
    17821 tgataacggt tcagacaggt ttaaagagga ataagaaaat gacagacaac actcgtttac
    17881 gcatagctat gcagaaatcc ggccgtttaa gtgatgactc acgcgaatta ctggcgcgct
    17941 gtggcattaa aatcaatctt cacacccagc gcctgatcgc gatggcagaa aacatgccga
    18001 ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct ggtaatggac ggcgtggtag
    18061 atcttgggat tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg
    18121 gtgaagatcc acgctacttt accctgcgtc gtctggattt cggcggctgc cgtctttcgc
    18181 tggcaacgcc ggttgatgaa gcctgggacg gcccgctctc cttaaacggt aaacgtatcg
    18241 ccacctctta tcctcacctg ctcaagcgtt atctcgacca gaaaggcatc tcttttaaat
    18301 cctgcttact gaacggttct gttgaagtcg ccccgcgtgc cggactggcg gatgcgattt
    18361 gcgatctggt ttccaccggt gccacgctgg aagcaaacgg cctgcgcgaa gtcgaagtta
    18421 tctaccgctc gaaagcttgc ctgatccagc gcgatggcga aatggaagaa tccaaacagc
    18481 agctgatcga caaactgctg acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat
    18541 acatcatgat gcacgcaccg accgaacgtc tggatgaagt catcgccctg ctgccaggtg
    18601 ccgaacgccc aaccattctg ccgctggcgg gtgaccaaca acgcgtagcg atgcacatgg
    18661 tcagtagcga aaccctgttc tgggaaacga tggaaaaact gaaagcgctg ggtgccagtt
    18721 caatcctggt cctgccgatt gagaagatga tggagtgatc gccatgagct ttaacacaat
    18781 cattgactgg aatagctgta ctgcggagca acaacgccag ctgttaatgc gcccggcgat
    18841 ctccgcttct gaaaacatta cccgcactgt taacgatatt ctcgataacg tgaaagcacg
    18901 cggcgatgag gccctgcggg aatacagcgc gaagtttgat aaaaccacgg ttaccgcact
    18961 gaaggtgtct gctgaggaaa ttgccgccgc tagcgaacgc ctgagcgacg agctaaaaca
    19021 ggcgatggcg gtggcagtaa agaatattga aaccttccac actgcgcaaa aactgccgcc
    19081 ggtagatgta gaaacgcagc caggcgtgcg ttgccagcag gtcacgcgcc cagtcgcttc
    19141 agttgggttg tatattcctg gcggctccgc cccgctcttc tcaacggtat taatgctggc
    19201 gactccggcg cgtattgcgg gctgtaaaaa agtggtgctg tgctcaccgc cgccgattgc
    19261 cgatgagatc ctttacgcgg cgcagctgtg cggcgtgcag gacgtgttta acgtcggcgg
    19321 cgcacaggcc attgccgcgc tggcgtttgg tactgaatct gtgccgaaag tggacaaaat
    19381 cttcgggccg ggtaacgcct ttgtcaccga agcaaaacgc caggtaagcc agcgtctgga
    19441 cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg ctggtgattg ctgacagcgg
    19501 cgctacgccg gatttcgtgg cttctgattt gctttctcag gctgaacacg gcccggactc
    19561 acaggtgatt ttactgacgc ccgatgctga tatggcgcat caagttgccg aggccgtcga
    19621 acgccaactg gcagaactgc cgcgtgccga aaccgcccgt caggcactga acgccagccg
    19681 cctgatcgtg actaaagatt tagcgcagtg cgtagagatc tccaaccagt acggcccgga
    19741 gcacctgatc atccagaccc gcaacgcccg cgatctggtc gatggcatca ccagcgccgg
    19801 ttcggtattt cttggtgact ggtcaccgga atccgcaggc gactatgctt ctggcaccaa
    19861 ccacgttctt ccgacctacg gctataccgc gacctgctca agccttgggc tggctgattt
    19921 ccagaaacgg atgaccgtac aggaattaac cccacaaggt ttctctgccc tggcctcaac
    19981 cattgaaaca ctggccgctg ctgaacgact gactgcccac aaaaatgctg tcaccctgcg
    20041 cgttaacgcc ctgaaggagc aagcatgagc actgacaact ttctcagcgt cactgattta
    20101 gcccgtaaga atgttcgcga gttaacaccc tatcagtctg cccgccgtct cggcggtaac
    20161 ggcgatgtct ggctcaacgc gaatgagtac ccaaccgccg ttgagttcca gctcactcag
    20221 caaacgctga accgctaccc tgaatgccag ccgaaagcag tgattgagaa ttacgcgcag
    20281 tatgctgacg tcaaaccgga gcaggtactg gtcagccgcg gcgcagatga agggatcgaa
    20341 ctgctggtgc gcgctttttg cgaacccggt aaagatgcca ttctctactg cccgccgact
    20401 tacggcatgt acagcgtcag cgccgaaacg attggcgttg agtgccgcac cgttccgacc
    20461 cttgataact ggcaactgga tttgcaaggc atcgcggaca agcttgatgg cgtgaaggtg
    20521 gtctacgtat gcagccctaa taacccaacc ggccagctaa ttaacccgca ggatctgcgt
    20581 tcactgctcg acatgacgca gggtaaagct atcgtggttg ccgatgaagc gtatatcgag
    20641 ttttgcccgc aggccacgct ggcaggctgg ctgtctgagt atccgcatct ggtgatatta
    20701 cgcacgttgt ccaaagcgtt tgcgctcgcc ggtttacgct gcgggtttac gctggcgaat
    20761 gaagaggtta tcaatctgct gttaaaggtg attgcccctt acccactctc tacgccggtg
    20821 gcagatatcg ccgcccaggc gctgacgccg caagggattt ccgccatgcg taaacgcgta
    20881 gagcagatcc tggaagagcg tcagtatctg gtagataacc tgaaaacaat tcgctgtgta
    20941 gagcaggtat tcgattctga aaccaactat gttctggcgc gcttcaccgc atcaagtagc
    21001 gtatttaaat ctttgtggga tcagggcatt atcttacgag atcagaataa acaaccttcg
    21061 ttaagcggct gcctgcgaat tactgtcgga acccgtgaag agagccagcg cgtcactgac
    21121 gccttacgtg cggagcaagt atgagccaga agtatctttt tattgaccgt gatggaaccc
    21181 tgatttccga accgccaagt gactttcagg tcgatcgctt tgacaaactc gccttcgagc
    21241 cggaagttgt tcccgttttg ctgaaactgc aaaaagcagg ttttaagctg gttatgatca
    21301 ctaaccagga tggactcgga acccagagtt tcccgcaggc tgattttgac ggtccgcata
    21361 acctgatgat gcaaattttc acctctcagg gcgtaacgtt tgatgaggta ttgatctgcc
    21421 cgcacttgcc agcggacgag tgtgattgcc gtaagccaaa agtgaagctg gttgaacagt
    21481 atctggttga acaggccatg gatatggcga acagctacgt cattggtgat cgggcgaccg
    21541 atattcagct cgctgaaaac atgggcatta gcggattgcg ttaccatcgc gataccctga
    21601 actgggcgat gatcggcgag cagttgacca aacgtgaccg ctacgcacac gttgagcgta
    21661 atactaaaga aacgcagatt gacgtcaaag tctggctgga ccgcgaaggc cgcagcaaaa
    21721 tcaatactgg cgttggcttc tttgaccaca tgctggatca aatcgccacc cacggcggtt
    21781 ttcgcatgga gattgccgtc aagggcgatc tgtatataga cgatcaccac accgttgaag
    21841 ataccggtct ggcgctgggc gaagccttaa agctggcgtt aggcgataaa cgcggcatca
    21901 accgttttgg ttttgtgctg ccgatggatg agtgtctggc acgttgcgcg ctggatatct
    21961 ccggccgtcc acacctggag taccgcgcgg agtttaccta ccagcgcgtt ggcgatctga
    22021 gcacggagat gatcgaacac ttcttccgtt cactctctta cactatgggc gtcaccctgc
    22081 acctgaaaac caaaggcaaa aatgaccacc accgtgtgga gagcctgttt aaagcctttg
    22141 gccgtacgct gcgtcaggcg attcgtgtag aaggcgacac gctgccctct tcaaaaggag
    22201 tgctgtaatg aacgtggtga ttctggacac cggctgcgcc aacctgcact cggtaaaatc
    22261 agccgtcgca cgccacggat atgatcctgt tgtcagccgt gacccggacg tggtgctgcg
    22321 cgccgataaa ctgtttctgc cgggcgtcgg caccgcgcag gcggctatgg accagttgca
    22381 cgagcgtgag ctgattgaac tgattaaagc ctgtacgcaa ccggtactgg ggatctgttt
    22441 aggtatgcaa ctgctgggcc gtcgcagcga agagagtaac ggtgtctccc tgctgggcat
    22501 tattgagcag gacgtcccta agatgaccga tttcggtctg ccgctgccgc atatgggctg
    22561 gaatcgcgtc tatccgcagg cgggaaatcg tctgttccag ggcattgaag atggcgccta
    22621 tttctacttt gtacacagct acgctatgcc ggtaaacccg tggaccatcg cccagtgcaa
    22681 ttacggcgaa ccgtttaccg cggcagtaca aaaagacaat ttctttggcg tacagtttca
    22741 cccggaacgt tcgggcgcgg ctggggcaca gttgctgaaa aactttctgg agatgtaatg
    22801 attattccgg cattagattt aatcgacggc acggtggtgc gtcttcatca gggcaattac
    22861 gctaagcaac gtgattacgg caacgatcct ctcccccgtt tgcaggatta tgcagcccag
    22921 ggtgctgaag tgctgcacct ggttgattta accggcgcga aagacccggc gcagcgccag
    22981 atcccgcaga tcaaaacgct ggtcgctggc gtcaatgttc cggtacaggt tggcggtggc
    23041 gtgcgtacgg aagcagacgt ggcggcgcta cttgacgcag gtgtggcgcg cgtggtggtg
    23101 ggttcgacgg cggtaaaatc ccccgagata gtcaagagct ggtttgcccg ttttggtgcc
    23161 gatgcgctgg tactggcgct ggacgttcgt attgacgagc acggtaataa acaggttgcg
    23221 gtcagtggct ggcaggaaga ttccggggtt tcactggaag aactggttga gacctattta
    23281 cccgtcggcc tgaagcacgt gctgtgcacc gatatctccc gcgacggtac gctggcaggc
    23341 tcaaacgtcg cgctgtatga agaagtatgc gccaaatacc cgcaggtggc gtttcagtca
    23401 tctggcggca ttggcggaat cgaggacgta gcggccctgc gcgggaccgg tgttcgtggt
    23461 gtgatcgtcg gacgagccct gctggaaggt aagttcaccg ttgaggaggc cattcaatgc
    23521 tggcaaaacg cataattcct tgtctggacg tccgtgatgg gcaggtggta aaaggtgtcc
    23581 agttccgcaa tcacgagatt attggtgaca tcgtcccgct ggcaaaacgc tatgccgatg
    23641 aaggtgcaga tgaactggtg ttctacgata tcaccgcttc cagcgatggc cgcgtagtgg
    23701 ataaaagctg ggtatcacgc gtggcggaag tgattgatat tccgttctgc gtagccggcg
    23761 gcattaaatc catcgacgat gcggcgcgca ttctctcctt tggggcggat aaaatctcca
    23821 tcaactcccc ggcgctggcc gaaccggaac ttatcacccg tctggcggat cgctttggcg
    23881 tgcagtgcat cgtggtgggc attgatacct ggtatgacgc cgacaccggt aaatatcatg
    23941 taaatcagta tacgggagat gaaagccgta cccgcgtcac gcagtgggaa acactggact
    24001 gggtgcagga ggtccaaaaa cgcggcgcgg gcgaaatcgt cctgaacatg atgaaccagg
    24061 acggcgtacg taacggttat gacctggaac aactgaaaaa agtgcgtgac gtttgccacg
    24121 taccgctgat tgcctccggc ggtgcgggca ccatggaaca cttcctcgaa gccttccggg
    24181 atgccgacgt tgacggcgcg cttgccgcct ccgtcttcca caaacaaatt atcaatattg
    24241 gcgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt taacgaagca
    24301 acaatgccgc gagctggact gggaaaaaac cgacggactg atgccagtca tcgtgcagca
    24361 tgcggtttcc ggtgaagtat tgatgctggg gtatatgaac ccggaggcgc tggataaaac
    24421 gctcgaaagc ggcaaagtga cctttttctc acggaccaag cagcgcctgt ggaccaaagg
    24481 agaaacctcc ggtcacttcc tgaatgtggt gagcattgcg cctgactgcg acaacgacac
    24541 cctgctggtg ctggttaacc cgattggccc aacctgccat aaaggcacct caagctgctt
    24601 tggtgacgcc agccaccagt ggctgttcct gtatcagctt gagcaactgc tggccgaacg
    24661 taaaaccgct gacccagcca gctcctacac cgcgaaactg tatgccagcg gcaccaaacg
    24721 catcgcgcag aaagtgggcg aagaaggcgt agaaaccgcg ctggctgcga ccgtgaacga
    24781 ccgttttgag ctgacaaatg aagcctctga tctgatgtat cacctgctgg tgctgctgca
    24841 agaccaggac ctcgacctga cgacggtgat tgagaattta cgtaagcgtc atcagtaagt
    24901 tgcagacgta aaaaaaaccg ggcgctgccc ggttttttgt tatcagcttt agacttactc
    24961 agctttggct ttgtaaccgc gcagagcatt tcgcccaagc accacaccgg caccaatcat
    25021 cccaccgatc agaactgcca gcacgagggt aatcgctttt ttcgggctgt cacgacgaat
    25081 cggtaacgtt ggtttcatta cataacggta ggcgtgcacg ctgtcaggtg ccacttctaa
    25141 tgcctgcacc tccaacaga
//
LOCUS       DAZUFB010000064        23827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZUFB010000064 DAZUFB010000000
VERSION     DAZUFB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.064"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..1589
                     /locus_tag="ACHY3M_002997"
                     /pseudo
     CDS             23..1589
                     /locus_tag="ACHY3M_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            1589..2797
                     /locus_tag="ACHY3M_002998"
     CDS             1589..2797
                     /locus_tag="ACHY3M_002998"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4317875.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2766..3362
                     /locus_tag="ACHY3M_002999"
     CDS             2766..3362
                     /locus_tag="ACHY3M_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4317876.1"
                     /translation="MNNTDLIHLIKRFMRNEHKVVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRAYFGLYHPGGALGIKANS"
     gene            3434..4381
                     /locus_tag="ACHY3M_003000"
     CDS             3434..4381
                     /locus_tag="ACHY3M_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4317877.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(4461..4537)
                     /locus_tag="ACHY3M_003001"
     tRNA            complement(4461..4537)
                     /locus_tag="ACHY3M_003001"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4501..4503),aa:Met,seq:cat)
     gene            4746..5843
                     /gene="mltA"
                     /locus_tag="ACHY3M_003002"
     CDS             4746..5843
                     /gene="mltA"
                     /locus_tag="ACHY3M_003002"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFS4317878.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            5920..6726
                     /gene="tcdA"
                     /locus_tag="ACHY3M_003003"
     CDS             5920..6726
                     /gene="tcdA"
                     /locus_tag="ACHY3M_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFS4317879.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(6777..7220)
                     /gene="csdE"
                     /locus_tag="ACHY3M_003004"
     CDS             complement(6777..7220)
                     /gene="csdE"
                     /locus_tag="ACHY3M_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFS4317880.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(7220..8425)
                     /gene="csdA"
                     /locus_tag="ACHY3M_003005"
     CDS             complement(7220..8425)
                     /gene="csdA"
                     /locus_tag="ACHY3M_003005"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFS4317881.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            8617..8844
                     /gene="ygdI"
                     /locus_tag="ACHY3M_003006"
     CDS             8617..8844
                     /gene="ygdI"
                     /locus_tag="ACHY3M_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFS4317882.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9195..10112
                     /gene="gcvA"
                     /locus_tag="ACHY3M_003007"
     CDS             9195..10112
                     /gene="gcvA"
                     /locus_tag="ACHY3M_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFS4317883.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10131..10526
                     /gene="ygdD"
                     /locus_tag="ACHY3M_003008"
     CDS             10131..10526
                     /gene="ygdD"
                     /locus_tag="ACHY3M_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFS4317884.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10519..11619
                     /gene="rlmM"
                     /locus_tag="ACHY3M_003009"
     CDS             10519..11619
                     /gene="rlmM"
                     /locus_tag="ACHY3M_003009"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFS4317885.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWLVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11663..12394)
                     /gene="fucR"
                     /locus_tag="ACHY3M_003010"
     CDS             complement(11663..12394)
                     /gene="fucR"
                     /locus_tag="ACHY3M_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFS4317886.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12452..12874)
                     /gene="fucU"
                     /locus_tag="ACHY3M_003011"
     CDS             complement(12452..12874)
                     /gene="fucU"
                     /locus_tag="ACHY3M_003011"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFS4317887.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(12876..14294)
                     /gene="fucK"
                     /locus_tag="ACHY3M_003012"
     CDS             complement(12876..14294)
                     /gene="fucK"
                     /locus_tag="ACHY3M_003012"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS4317888.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14403..16178)
                     /gene="fucI"
                     /locus_tag="ACHY3M_003013"
     CDS             complement(14403..16178)
                     /gene="fucI"
                     /locus_tag="ACHY3M_003013"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFS4317889.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(16211..17527)
                     /gene="fucP"
                     /locus_tag="ACHY3M_003014"
     CDS             complement(16211..17527)
                     /gene="fucP"
                     /locus_tag="ACHY3M_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFS4317890.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            18074..18721
                     /gene="fucA"
                     /locus_tag="ACHY3M_003015"
     CDS             18074..18721
                     /gene="fucA"
                     /locus_tag="ACHY3M_003015"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFS4317891.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            18749..19897
                     /gene="fucO"
                     /locus_tag="ACHY3M_003016"
     CDS             18749..19897
                     /gene="fucO"
                     /locus_tag="ACHY3M_003016"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4317892.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(19952..20707)
                     /gene="xni"
                     /locus_tag="ACHY3M_003017"
     CDS             complement(19952..20707)
                     /gene="xni"
                     /locus_tag="ACHY3M_003017"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFS4317893.1"
                     /translation="MAVHLLIVDTLNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(20819..22186)
                     /gene="sdaB"
                     /locus_tag="ACHY3M_003018"
     CDS             complement(20819..22186)
                     /gene="sdaB"
                     /locus_tag="ACHY3M_003018"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFS4317894.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(22244..23533)
                     /gene="sdaC"
                     /locus_tag="ACHY3M_003019"
     CDS             complement(22244..23533)
                     /gene="sdaC"
                     /locus_tag="ACHY3M_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFS4317895.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5979 a         5621 c         6159 g         6068 t
ORIGIN      
        1 acattttcat gagataacca gtatgaaaca aaagaaagcc tggagttttt ttcagagcct
       61 ggggaaggca tttatgtatc ccatcgctct gttaagtgta tgtggtatga tgttagggct
      121 gggaagtggt ttagccagcg atgatatggg aaaattcatt ccagtgttgg ctattccgtt
      181 aattaaaacc atacttgatt ttattgttag ccttcgttaa tttacctgta ttgtttgcga
      241 tagctatacc tttgggatta ttaaaagaga aagatgataa agcctatggc gctttttctg
      301 gcttaattgg ctttatggcg atgcatcttg gaacgaactt ttatcttaaa cagcacgact
      361 tattggtagc tgctgaccaa atgtcgacac atgggcaaac catcatactc gggatccagt
      421 cctacaatac cagcgtgttg ggtgggattg tggcagggtt attagtcgcc agcatgtata
      481 aaaagatcgt taacttacgt attcctgaat cattagggtt ttatagtggc ccacgccttg
      541 tgcctatcat tacactgatt gtgatgagtg gatttggtct gttcattcct tttatctggc
      601 cgccattttt caatcttttc atgctcattg gtcactggat ttcaacttcc ggtcctgttg
      661 gttatttctt ctatgctgtt gccgaacgcg tgacgatacc ttttggctta aatcacttgg
      721 tgacatcagt tttccgcttt accccaatcg gcggatcggc agtgattggc ggtgaagagt
      781 attacggcac cctgaacatg tttatggcat acgtcaaaga gaatgcggtc attccgctgg
      841 atttggcggg gaaaatggag cagggcaaac tgatgattca gtatggtctg gctggagccg
      901 cgctggcgat gtatcgcact gctcatgctc aaaacagaag ggctatcaaa gcattgctga
      961 tttccggtgt gctcacggta attattggcg gcgtcagcga gccgattgaa tttctgttct
     1021 tatttgtcag cccgctgttg tttgtcttcc atgcctttat gaatggattg gctaacatgg
     1081 ttctgccata tatgggcgtg aagatgggct ttactggcga tctgattcaa tttattagct
     1141 ttggtgtctt acgtggcaca agaacgggat ggccattagc ggtatgtgtt gaagtggctt
     1201 atttcttcat ttattacttt gtgttccgtt ggaccattct caaatttaac ctgatgactg
     1261 taggccgtga agagtccagt cctgtcacgc tgaacgctca cgaagaaact gcaatagcta
     1321 acatcccagt tcatgataaa tcagagttgc aggcagcgga gcaaatggtt aaggctctgg
     1381 gtggtaaaga aaatattaaa tcactggata attgtgtaac tcgtttgcgt ttaactatcg
     1441 cggatatggg attgatagat gaggctgcaa taaaaagggc cggaggaatt gcggttgtga
     1501 aacttgatca aaatacctta caagtcatta tcggtacaaa agtcatcgcc ctacgtcggg
     1561 atatggataa ttacatgggg atatgttgat ggatattttt aatacctcaa tttctcgtaa
     1621 aggaacatat tgcacacaat gggacttttg tgaagacaga tttggtgtaa aagatgtatt
     1681 accattctct atttcagata tggatcttcc gataccggaa gctatcatca gaacattaaa
     1741 aaaacgtctg gaacacccga ttttagggta cagtcgttgg cagcatgatg actatctgaa
     1801 ttcaatagtc aattggtatc atcaacaata taaaacagag attcaaccag aatggatcac
     1861 ctatagccca agcgtgatgt attcaatatc taaggcaata gaaatattga cattgaatgg
     1921 agataatatt ctcattttca cgcctgttta taacgcattt tttgatgtga ttaagcataa
     1981 tggtcgaaac atgctaacaa cctctttgat gaaagaaaat gggagaggct acgttatcaa
     2041 ctggcaagac tttgagctta aaattaaaag cgcgaagatg gtgttattat gtaatcctca
     2101 taacccgaca ggaacggtct ggtcggaaga cgatcttcac agaatcgcag catgctgtgc
     2161 aaggcataat atttggttat gttcagatga aatacatagt gattttgttt ttaatcatag
     2221 tttcacgtca gcgctaacaa taaaagatga caaagtggtg gtgtttaatt cgatttcgaa
     2281 aacatttaat gttcctgcgc taaccggatc atatatgatt tcaactgacg ataattttaa
     2341 tcataagttc agaacgatat cgaggtatcg cgatttcgta aactccccat cagtactgaa
     2401 tgtaattgcg accattgttg cctataatga atgtgaagaa tggttaaagt cgttaaaggt
     2461 acatattgcc tctaacattc aatttacctt gcagtattta aatgaaaata ttccggaatt
     2521 gatcgtatgg ccagctgagg gctgctattt ttcgtgggtt gattgctcgg cccttggtta
     2581 tccctttgat aaattttacc gtcgattaat taacgaaggg agagtaggta ttatggcggg
     2641 gcatgtttat ggtactgaag gagagggcta tctgcgtctt aatgtggcct gcagtcgaga
     2701 aaaattatat atggggcttg cgcgccttgc gagtgtaatt aaaaatatta atcaggaaaa
     2761 agaaaatgaa caatacggat cttatccatc ttattaaacg ttttatgcgt aacgaacata
     2821 aagtagtaga agaggttatt gattcccccc tttcggaagt cgcgaattta attaaaatat
     2881 tacaatcttg ccagggaaaa gttgttttta ttggtgttgg gaaatctggc attattgcca
     2941 gaaaacttgc tgcaacattt gccagcaccg gaactccctc gttttttgtt cacggtacgg
     3001 aagcggtaca cggcgacctt gggatggtgg cgaaagacga tgttgttatt cttatttcaa
     3061 acagtggtga gacggcggaa atcctggcga cactgcctag tttgaaaaaa atgggtaatt
     3121 atttgatctc ttttacccgc agtcatcact catcgcttgc gataagttgt gatttatctg
     3181 ttgaaatccc cgtcaaaagt gaggcggata atttaggttt agcaccgtca tgctcgtcta
     3241 ctgttgttct ggttgttggt gatgcggtgg cgcttgcact ctctgaattg aaaaaattta
     3301 ctcgtgctta tttcggctta tatcatccag gaggtgcact cggcattaaa gcaaattcat
     3361 aatttcaaag tataaataca gtaaccggtc acgccatctt ttggggtggc gttcattgaa
     3421 gtgagtgtca gctatgaaaa atgttctggt caccgtcccc agcttttcag cacgctgtgt
     3481 ttccgccagc aagttgctaa gagagaataa ttttaatctt attattaaga ataatgtgga
     3541 gcatctgctt aaatcagaat ccactgcatt acgagaatct atttgcgcgg tcatcgccgg
     3601 aaaagattgt tatcaggcgg acacgctatc attgctgcct ggtgtgaaaa taatatcgag
     3661 attcgggacg gggatagata atattgatct tcgtgcagca caacaatccg gtattgtagt
     3721 caataatgcg gttgggatta attcaaatgc tgtcgcggaa tttatcattg gacttatctt
     3781 cgcgagcatg agaaatattc caggcagcta tcatgcgatg caaaatggct actggggtga
     3841 gtcgcatggc tatgaattac aaggcaaacg gattggcatg gttggctacg gcaatatagg
     3901 taaaaccctg gcaaaaaggt tatccggttt tgatgttgag cttttagctt atgacaaaca
     3961 acccgattat caggttgctg acaaagttgg agttcagttt gtatcaattg aagatatctt
     4021 tatgcagtcg catgtcatca ttgttctttt gcctttttct cctgagctgg agaacttcat
     4081 tagtcataaa tatttgtcga tgatgcgcaa tggtgctcta atcatcaatg ccgcaagggg
     4141 aaagttgctg gatgagggcg ctttactaca ggtgattgaa gaacgaaatg tgtttgcggc
     4201 gctggatgtg tttagctctg aaccgttggc gcaatttagc ccactgctac atgccaaaaa
     4261 tatcattaca acgccgcata ttgcggccgc caccgttgag tcataccagc aaaccggtac
     4321 acacgttgcc cagtcgataa ttgattactt cgcaggaagg gagataaaaa acgtgttgtg
     4381 agttcagcga acgaagtttt taaatacaaa aaaaaccgca atttgtggaa cacattgcgg
     4441 tttatcagca ttctaagtat tggttgcggg ggccggattt gaaccgacga ccttcgggtt
     4501 atgagcccga cgagctacca ggctgctcca ccccgcgtcc gtggatgcgc actatactcc
     4561 gctcgcgttt tcatgcaacc ttttttttac cccgatcacg gatttagcat gattttgaac
     4621 aaaaacaaac cactaaagcc tattttttgt gcaaaatttg cgtcagggag aggctgctca
     4681 tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt attttttaac ctgaagaaga
     4741 gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg cacggttgtg gcaatgcttg
     4801 ccgcctgctc ttccaaacca accgatcgcg gacagcaata taaagacggg aaatttaccc
     4861 agcctttctc tctggtgaac cagccagatg ccgttggcgc gccgattaac gccggtgatt
     4921 ttgccgagca aattaaccat atccgtaatt cgtcaccgcg tctgtatggc aaccagagta
     4981 atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg tgatacccgc aatatgcgcc
     5041 agttcggcat tgatgcctgg cagatggaag gtgccgacaa ctatggtaac gtgcagttta
     5101 caggctatta cacgccggta attcaggcgc gccatacccg ccagggcgag ttccagtatc
     5161 ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc tcgtgcggag atctacgcag
     5221 gggcgctgag tgataaatat attctcgctt acagcaactc cctgatggat aacttcatta
     5281 tggatgtgca gggtagcggg tatatcgact ttggtgatgg cagtccgctt aactttttca
     5341 gctatgcagg gaaaaacggt catgcctatc gcagcattgg taaggtgctg atcgaccgtg
     5401 gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg tcattggggc gaaacacaca
     5461 gtgaagccga ggttcgcgag ctgctggaac agaacccgtc tttcgttttc tttaaaccgc
     5521 aatcttttgc accggtgaaa ggggcaagtg cggtgccgct ggttggccgt gcttctgttg
     5581 cctccgatcg ttcaattatt ccgccaggta ctaccttgct ggcagaagtg ccgctgctgg
     5641 ataataacgg caaatttaac ggtcagtacg aattgcgtct gatggtggcg ctggatgtcg
     5701 gtggcgcaat caaaggccaa cacttcgata tctatcaagg gatcgggccg gaagccggac
     5761 atcgcgcagg ttggtacaac cactatggac gtgtctgggt gctgaaaacc gccccgggcg
     5821 caggtaacgt ctttagcggc tgatgtgtta ttctgtatac cgtttttacg gatacgacag
     5881 ggtgagggat aacctcaccc tttttaattc tgaggtgcta tgtctgtggt aattagtgat
     5941 gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg gtgaaaaagc gttgcaactg
     6001 tttgctgacg cgcatatttg tgtggttggt atcggtggtg tcggttcctg ggcggcggaa
     6061 gcgctggcgc gcacggggat tggcgcaatc acgcttatcg atatggatga tgtgtgcgtc
     6121 accaatacca atcggcaaat tcacgctctg cgcgataacg tcgggctggc aaaagcggaa
     6181 gtcatggcgg agcgtattcg ccagattaac ccggagtgcc gtgtaacggt ggtggatgat
     6241 ttcgtgacgc cggataacgt agcgcagtat atgagcgtgg ggtattcgta cgtgattgat
     6301 gccattgata gtgtacggcc caaagcggcg ctgattgctt attgtcggcg caataaaatc
     6361 ccgctggtca cgaccggtgg cgcgggtggg cagattgacc cgacgcagat tcaggttacc
     6421 gatctggcga aaacgattca ggatccgctg gcggcgaagt tgcgcgagcg cctgaaaagc
     6481 gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg tcgattgcgt gttttctact
     6541 gaagcgctgg tgtacccgca gtcagacggt actgtgtgtg cgatgaaagc cacggcagaa
     6601 gggccgaagc ggatggattg tgcatctgga tttggcgcgg caacgatggt gaccgccacc
     6661 tttgggtttg ttgcggtttc tcatgcgctg aagaagatga tggcgaaagc ggcgcgtcag
     6721 ggttaaacaa tcaggtcgga tgcgacgcga acgtcttatc cgacctggtt ctgtgcttaa
     6781 acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat tcaacccctg gctgcgtgag
     6841 gcgctaagct gcccacgcaa tcccagctca tcaaacaatg ccagcggcga ctgtgcctgc
     6901 aattcggcgg cggttttccc ctcaacggca gtcaacagca ccgccagcag gccgcgcact
     6961 atacgtcctt cgctgtcgcc aaagaagtgc attttaccgt tttcagccac ggtatatccc
     7021 agccagacgc ggttttcgca tccggcaatc tctttagcct gcgcttttaa ctcgtctggc
     7081 aaagccggaa gctgtttccc cagcatgatc aactggcgat atttatcttc ccattgcgtc
     7141 aacggtgcga aggtattgcg taacgtttct gcggttacgg ttgtgccgaa cggatgtccg
     7201 gcgaattgcg ggttcgtcat taatccacca ataattccag cgcgcggtca acggcattca
     7261 ccagcgcatc cacatcactc tttgtattat atggcgcaaa agaggcgcgc agtgtgccgg
     7321 ttacgcctaa ttctgccagt agcggctgag cgcaatgttg cccggcccgc aaggcaatac
     7381 cgtactccgc cagcagcgtc accatatcgc tgtgatgaac gccagcaaaa tcaaaggcca
     7441 gcaggctgga atcctggcag cggaatgaac ggaaacctgg acgtttcgcc agcgcatctt
     7501 ccgccagtgt tgctaagcta cggctccagt tttcggcctg gttgatatcg taatctgcca
     7561 gccattccag cgccgcgctt aatcctatga caccagccac atttggcgtt cctgcttcca
     7621 gtttccacgg cgcagattga gtcgtgaagc cgtcaaaact cacttcgtga accattttgc
     7681 cgccgcccag ccagggcgac atcgcttcca gcagttctga tttgccatac agcacgccga
     7741 tacctgtcgg gccatacagt ttgtgacctg aaaaagcata gaaatcaata ttcagttgct
     7801 gaacatccgc ggggaaatgc accgccccct gagcaccatc aaccatcacc accatcccgg
     7861 ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca accgccagta acgttcgaca
     7921 tctgacccaa cgccagaatc cgactacggg gagtaatcag ttctggcaac aaatcgacat
     7981 ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt ggctccagtt tgttgggcga
     8041 ccatcagcca ggggacgagg ttggcgtggt gttctgccac gctgacaata atctcatcgc
     8101 ccagttgcag acgcggacgc gcatagcatt gtgcaaccat gttgatggat tcagtggtgc
     8161 cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag taattgcgcc actttctctc
     8221 gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc aaactggctg cgatggacgt
     8281 ttccggcgct caggctgtaa aactgtcggg tggcttcaac cacggcttca ggtttaagcg
     8341 cggtcgcggc gctgtcgaga tagacgcccg catcctgtag agcgggaaac tgggcgcgaa
     8401 actgcgcggg attaaaaacg ttcatggtac tcctcggctt gatgcaccga tcgtgacgca
     8461 aaattcactt tgatacaagg agagtgctaa ccatcagaat tatctggata tcctggcgaa
     8521 gccgcgtaag taacatatgc tgaatagtgt taggcgttaa ttttagcctg ttaatcaata
     8581 ctattaagat ttattaatcg ctttaaggag aagatgatga aaaagactgc cgcaattatt
     8641 tctgcctgta tgctgacttt tgccctgagc gcctgttccg gttcgaacta tgtgatgcac
     8701 accaatgacg gacgtaccat cgtctctgac ggcaaaccac agactgataa cgataccggt
     8761 atgatttcgt ataaagacgc taatggcaac aaacagcaga tcaaccgtac tgacgtgaaa
     8821 gagatggtcg aactggatca gtaattcacg atcgcaaggt aaaaaaaagc accgcaatta
     8881 ggcggtgcta cattaatcac tatggacaga cagggtaaat gtacaggaag tgaaaaaagg
     8941 tagctttgct accatggtct gaatcgcaga ccaattgcaa acacaacaac acaacatcac
     9001 aaccgtaagc caaaagttca ccagaacacg cattccgata aaacttttcg ttccggctca
     9061 ggaagtgccg ccactatagg tatttgctgg tagaagctca acggacaatt tataatggct
     9121 cagattaaaa aaactaatag gttacacagt gtgatctaat tgttaaattc atttaacatc
     9181 aaagtttaaa agccatgtct aaacgattac caccgctaaa tgccttacga gtttttgatg
     9241 ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga gctttttgtg acccaggccg
     9301 cagtaagtca tcaaatcaag tctcttgagg attttttggg gctaaaactg ttccgccgcc
     9361 gtaatcgttc actcctgctg accgaggaag ggcaaagcta tttcctcgat atcaaagaga
     9421 tattttcgca attaaccgaa gcgacgcgta aactccaggc ccgtagcgcc aagggggcgt
     9481 tgacggtcag tttactcccc agtttcgcca ttcattggtt ggttccgcga ctttccagct
     9541 ttaattcagc ttatccggga attgacgttc gaatccaggc ggttgatcgt caggaagata
     9601 agctggcgga tgatgttgat gtggcgatat tttatggtcg gggcaactgg ccggggctac
     9661 gggtggaaaa actgtacgcc gaatatttat tgccggtgtg ttcgccgcta ctgctgacag
     9721 gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca tacgttatta catgatgcgt
     9781 cacgccgtga ctggcagaca tatacccgac agttggggtt aaatcatatc aacgttcagc
     9841 aagggccaat ttttagtcat agcgccatgg tgctgcaagc ggctattcac gggcagggag
     9901 tggcgctggc aaataacgtg atggcgcaat ctgaaattga agccggacgt cttgtttgcc
     9961 cgtttaatga tgttctggtc agtaaaaacg ctttttatct ggtttgtcat gacagccagg
    10021 cagaactggg taaaatagcc gcctttcgcc aatggatcct ggcgaaagcc gctgctgaac
    10081 aagaaaaatt ccgctttcgt tatgaacaat aatttacgta gggtacgacc atgaccagcc
    10141 gttttatgct gattttcgcc gccattagcg gcttcatttt tgtggctctg ggcgcttttg
    10201 gcgcgcatgt gttaagtaaa accatggggg ccgttgagat gggctggatc cagaccggcc
    10261 tcgaatacca ggcgtttcat acgctggcga tcttaggtct ggcggtggca atgcagcgtc
    10321 gcatcagtat ctggttttac tggagtagcg ttttcctcgc gttaggcacg gtgctgttca
    10381 gcggcagcct ttattgcctg gcactgtccc atctgcgttt gtgggcgttt gtcactccgg
    10441 ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt agttggtgct atccgtttaa
    10501 agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt gccgtccggg ctttgaaaaa
    10561 gagtgcgccg cagaaattac cgataaagcc ggccagcggg aaattttcgg ttttgcccgc
    10621 gtgaaagaga atgcgggtta tgtcatttat gaatgttatc aacctgatga tggtgataag
    10681 ttaatccgtg agctgccgtt cagttcatta atttttgccc gccagtggtt tgtggtgggg
    10741 gaactcctgc agcatttgcc gccagaagat cgtattaccc ccattgtcgg catgttacag
    10801 ggcgtagtag agaagggcgg tgaactgcgt gttgaagttg ccgataccaa cgaaagcaaa
    10861 gagttgctga aattctgccg taaattcacc gtgccgctac gcgctgcctt gcgcgatgcc
    10921 ggggtgctgg cgaactatga aacgccgaag cgtccggttg tgcatgtatt cttcattgca
    10981 ccaggttgct gctataccgg ttactcatac agcaacaata attcgccgtt ctatatgggc
    11041 attccgcgcc tgaaatttcc ggcagatgcg ccgagtcgtt ccacactcaa actggaagag
    11101 gcatttcatg tgtttattcc tgcggatgag tgggatgaac gcctggcgaa cgggatgtgg
    11161 gcggtggatt tgggcgcttg cccaggcggc tggacctacc aactggtgaa gcgcaacatg
    11221 tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc tgatggatac cgggcaggtg
    11281 acgtggctgc gggaagacgg tttcaaattc cgtccgacac gcagcaatat ctcctggttg
    11341 gtatgcgata tggttgaaaa accggctaaa gttgcggcat tgatggcgca gtggctggtt
    11401 aatggctggt gccgtgaaac cattttcaat ctcaaactgc cgatgaaaaa acgctacgaa
    11461 gaagtatcac acaatctggc gtatattcag gcacagcttg atgaacatgg cataaatgct
    11521 cagattcagg cgcggcagtt gtatcacgat cgcgaagaag tgacggtgca cgtccgccgc
    11581 atctgggctg cggtgggtgg tcgtcgcgac gagcgataac aggcaaggcc ggggcgtcgt
    11641 ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga agttgcaacc tggcgctcat
    11701 cagaaataat gtgcgttacc tcatccagat gcccgatgcg ggcttcccct gaacgattaa
    11761 atttactttt atcaatcaat aacaacgatt gcgcggcacg ttttaatagc atcgatttgt
    11821 aatcagcgtt gatggcattg gagtcccaca gtgcgccgct gctatcgatc ccttcacaag
    11881 aaaaaataaa cagatcgatt tccagcgatt taagttggga aatcagcgag ggattgacgt
    11941 aacagccata tttgcgctca agtgtgccgc cggaactgat cagttgaatg cgttcgcgtt
    12001 taccgagttc atggcaaatc ggatggctat tggtgaagac ctgaatgttg atgtcaggca
    12061 actggcgtgc cagataccag caagttgaac tggcgtctaa ggctatcacc atcccttctt
    12121 caatccacgc gagcgcctcg cgcgcgatat ctgctttatg cgcataatgg cttttcagcc
    12181 ggatgtgaaa gggatcgcca ctgtcttgat tttgacggtg gatatattta gcgcgtccat
    12241 gattgcgcag aattttaccc tgcgtctgta attcattgag atcgcgacga atggtttctt
    12301 tactgacctt tagctgttca gagagagctt ccgtggtcag gctggtatgg ttcagcagca
    12361 ggtcgactat cgcttgctgg cgtgccgctt tcatattttt caccccatca ttcgataacc
    12421 gcccgcgtca tgcgggcgta ccggtatgag attacggtgt tacccctttt tttaaaagaa
    12481 tattcccgta cttcgctcgt tcgcctgtga taacgatcgc aaaggctttt tgcgcccgtt
    12541 cataaaacgc aaaacgattg atgcggatga tgtcaggaca cggggcttgt agtgaaagcg
    12601 cattacggta acgtcgttct acttcaggat cgagagtgtc accttctacc gctgccatca
    12661 tcaccagcgg cggggcataa ctgtccagct caaataacgg gataatcgcc tggagcaagt
    12721 cgctcaccaa caggccatca gcgcggatca cctgcggtcc catcgaatgg gcgggaaagt
    12781 gagcatcgga aaaaataatt tcatctccat gtcccatctc tgccagcact ttcaatagtt
    12841 cgggagaaat taacggcgaa attgttttca gcatttcaca cttcctctat aaattcaggt
    12901 tcagtttgcg ggtagaaata acggtactga taatgaatct gtgcacgggc ttcttccggg
    12961 ctgttgaatt cccctacgcc ataccaaccg aacagcgcag ctcctgcgac ggtcgtttcg
    13021 gcatcgtcga gaacttttac cggaatatca agcatattgg ctttaatctg attccacaat
    13081 gtgttgcgac ttcctccacc gactaacaat aattcagagg ccttaaagtg cccgattttt
    13141 tccagcatct gtagattgcg ctgtaattgc gcagttaacc cttccagcgc cgcgcgatag
    13201 aaatgccccc gcgtggtatt aagcgtcact ccttgccagc cagcgttctg acacgacaat
    13261 aaatcacact gcatttttac gccatccgcg ccaggcgcga tcagacgagc ttcttcaatc
    13321 aacatttgcc agggtgtttc agccgtccag aacagttttc tcacccattc cagcacgccg
    13381 gatgccagcc attgcatacc tgggttatac aaccctgcct ggctatccag ttcgcaggtg
    13441 gaaccggcgt actgacttaa cagcgaagta tcaacctggg cgctacggac cattaaaatt
    13501 tcccatgtac cggaagagag cacgggttca ttttggtcgg caccagcgcc aaaaagggcg
    13561 aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa cgggtaagcc gagcattgct
    13621 gcggcgctgt tctgtagcgt accaatctgt tcacccgctt ccaccagacg agggaagagt
    13681 cggcgtggaa taccggtggc ttgtaaaatt tgcggactga aatcgcgttg ctggatatcc
    13741 agcatctggc tggttccggc catcgtgata tcagtagtga attcgccggt taaacggtgg
    13801 ttaatcagcg acgaaataaa gagccaggcg tgcgcgcgtt ccagcagttg tggatgattt
    13861 tctttcaacc acaccaactt atataacgta ttgaaactaa aggctccgac gccagaaata
    13921 gcctgcaacc gctgtgcgga gatcaactgt tcaatattgt ccataaccgc tgctgttcgc
    13981 ggacatttcc agctaatgat cggatagagc agattgcctt gcttatctac cagagcgcca
    14041 tccacaccaa aggtggtgac ggcgataccg cggatgtggc attcagtcag ttcactattg
    14101 atttgccgac agcaatcagc aaagcgttgc aaaatggcgt ctaaagacca ctggtgccag
    14161 gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg aggcgcgggc aacaattttt
    14221 ccctgccgat taaccgcgat ggccctgaca ttggtcgcgc cacagtcgag taccaggata
    14281 acttcttgtt tcataatcgc tcctgcaata tagccggata acattgctta tccggctaac
    14341 cactcttgct tgttcaggcc cggtagccgg agcgaccggg catcacatca gggagtaatg
    14401 tattaacgct tgtacaacgg gccgtagttc tggcaagcgc ggtaatcctg gccttcaata
    14461 tccatgccgt gcgcagccca ggcagaagga cgatacactt tggtctcttc aacgttgtgc
    14521 atacataccg ggatacgcag catggaggcg agagtgataa agtctgcgcc aacgtggccg
    14581 atggtcagaa ccccatggtt agcgccccag ttcgccatta ccgagtacac atccgtaaac
    14641 ggccctttac cggtgaggcg cggtgcaaac caggtggttg gccaggttga gttggtgcgt
    14701 ttgttgagga tgtcatgcac atccttcggc aattccacgc tccagccttc cgcgatttgc
    14761 agtaccggtc ccaggccttt gatgatgttg acacgggtca tggtgaacgg gacgccgcct
    14821 tcggtaagga agcgggaaga gtaaccgccg ccacggaagt attcgtggat cgccgggcac
    14881 cattcggtag cggcgaggca agcgtcagcc tcttgctgag agatttccca gtgcggcttc
    14941 atcgtcgggt taccttcgct gtcgcgttgt ttacaggaac cgtccagcgc agcagaaccg
    15001 gagttgatca aatggatgat gccgtgttct gccagtccat cgagtttatg ccccgttaca
    15061 cgctcaattg cttctggtga ccagtaggta cgcacatcgg caaatacctg agcggtgccg
    15121 gtgagctggt gacccattag cattgccacg ccgttaagac tgtcgttttc ggtcgccacg
    15181 acaaagggtt cgcgcacgcc attccagtca aatgaactgt taaggatcgc ttcggcggtg
    15241 tcaccattgg gatattgatc ggtccagtga cgttgcccct ggaagcccgc agcgatggcg
    15301 ttgtagccaa gtgattcttc cacgcgacca atatcggcca gtttgctgtt gccttgcatc
    15361 atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca gaactgcgcg gctttgctcg
    15421 gcattgcgtt gatactgttt gttattttca tcttcgccat agcggaagtt tttatcagcc
    15481 caggccagtg ccatttccag ttcggcttcg tcgtaaatct tctgatcgat acggcgacgc
    15541 agttcggtca tatccaccgc ctggactttc attcccagcc aggattcaaa gaagttgtga
    15601 tcaacaatgg aaccggcgat acccatcgaa acgccgccca gcgacagata gcttttacct
    15661 ttcatgctgg cgacggccaa accggcgcgg gcaaagcgca gcagtttttc ttcaacatcg
    15721 gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac cgtaaatgga gaatgccggg
    15781 atgcctttct ggctgtgagc tgccagagcc gctgccaggt aaacagcgcc gggacgttca
    15841 gtgccgttaa agccccaaat ggctttcggg cgggttggat ccatgtcgat ggtttcactg
    15901 ccatagcacc agcaaggcgt taccgtaatg gtgaggccta cattctgact gctgaatttt
    15961 tcttcgcaag cagcggcttc agccataccc gcgatacagg tatcggaaat gacacactcg
    16021 acggcagctc cgcaggcatg gcgcagtttc tcggtcagca gtgcggccgt agctttcgcc
    16081 atattcattg tttgttcttc aagcgactca cgaacaccca tgcgacgacc gtcaataacc
    16141 gggcggatac caattttcgg taagctgatt tttttcatta cagattcctc actttattcg
    16201 gaaaatatgt tcagttagtt gccgtttgag agcggaaacg ggcaaagata aagatgaccg
    16261 cgaagcagag tgcggggatc agttcagcag tggggatgtt gcccgccgcg tcactgacaa
    16321 aacccatgac cggagtgaca ataccgccgc caatgatggt cataacgatg aaggacgaac
    16381 cgtatttggt gtcctggccg agattcttaa tgcccagcga gaagattgtt gggtactgaa
    16441 tcgacataaa ggcgctgcat aaagtcaggg ctattaagcc cacatgaccg ccagcgaagg
    16501 ctgagatcag gcacagtgcc atagcgatta atgcgtaggc ggccaggact ttgtgtggtg
    16561 cgaagcgact gatgagccag gtaccggtga aacgaccaat aaagaagcac accatggttc
    16621 cggttaaata gttagcggca aagcctgcag tcatacctgg aatttcttct acagcgtagc
    16681 gaatcaaata gctccagcag gccgtttgtg cgccaacgta gcagaattgc gctaatactg
    16741 cccagcgcca gtggcgaata cgcgccaggc gagaaagcga tgcggagaac gatccttgtt
    16801 tggcgtcact gtgattatca ctctgcaatg ccgggaattt cgtcagcatg atcagcaggg
    16861 cgaccagtaa cacgatagcc acgatgatca tataaggtgt ctgtaccgat aataccaggc
    16921 tgtgtttata cgcactcaat tgctctggag acattttatc gagaacgtct tgcgattgat
    16981 gtggcacgtt agacaaaata agactttgcc caaagacaac cgcgataatt gcgccaaacg
    17041 agttaaatgt ttgcgcaaga tttaagcgga agtgaccact gctttccggc cctaataccg
    17101 taacaaaagg gtttgcggca gtttccagac aacctaatcc ggctgcaata ataaataggc
    17161 caactaaaaa taaggtgtag ttcattattt ctgcagcggg ccagaataat gcagcgccca
    17221 aggcatataa aaataatccg gtaataatcc ctgctttata actgagtttt ttcatcaata
    17281 tcccagcagg gattgggata atgaaataac caaagtaaaa ggccgattgg atcaggccag
    17341 cctggaaatt tgtcagcgta aaagcctgct ggaattgagg taataaaatg tcgttaaggt
    17401 tattggctac cgcccaaaga aaaaacagtg agcacagcag cgcgaatgga ataatgtaac
    17461 ttctgctttg ccctgcatct ttatctaccg cacggtaact ctgcgtttgt attgatgtgt
    17521 ttcccatagc atcctcttag gttcagaagc ttaagcacgt aatcggttat gttttcaaaa
    17581 gtgcaaaacc agcgagttcg ggcttcatat cagtgaatat ttgcctgtct gaaatgaact
    17641 catggattca ttttcaaata aaaaacgctc tgtaatatga cggcggtcac acttattagc
    17701 tggaaggtga ctttatgtga ctaccatcac tttaataatc gaaatttcat gcccgtttca
    17761 gaaaataaat cataaaaaac acaaaccaga tgaccgtatt aattgtttat gccgaaaacg
    17821 ggcattcctg gcttgagtga atagaaaaat gaccgttttg ataaatattt ttaagagcgg
    17881 tcatttttaa tggggttata aaaagagaac ttgcagttat ttactgtgat tagttgaacc
    17941 aggtcacaaa atataagctt gtgaatttct ttctggttaa tcaggaagaa gatggtgggt
    18001 aattaaacgg ctaattcaat agtgtgaaag gaacaacatt attgccctgt tttgaatcag
    18061 agagaggtag ctaatggaac gaaataaact tgctcgtcag attattgaca cttgcctgga
    18121 aatgacccgc ctgggactga accaggggac agcggggaac gtcagtgtac gttatcagga
    18181 tgggatgctg attacgccta caggcattcc atatgaaaaa ctgacggagt cgcatattgt
    18241 ctttattgat ggcaacggta aacatgagga aggaaagctc ccctcaagcg aatggcgttt
    18301 ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg gttgttcaca atcatgccgt
    18361 tcattgcacg gcagtttcca ttcttaaccg atcgatcccc gctattcact acatgattgc
    18421 ggcggctggc ggtaattcta ttccttgcgc gccttatgcg acctttggaa cacgtgaact
    18481 ttctgaacat gttgcgcagg ctctcaaaaa tcgtaaggca actttgttac aacatcatgg
    18541 gcttatcgct tgtgaggtga atctggaaaa agcgttatgg ctggcgcatg aagttgaagt
    18601 gctggcgcaa ctttacctga cgaccctggc gattacggac ccggtgccag tgctgagcga
    18661 tgaagagatt gccgtagtgc tggagaaatt caaaacctat gggttacgaa ttgaagagta
    18721 atttcgtaaa gcaacaagga gaaggatgat ggctaacaga atgattctga acgaaacggc
    18781 atggtttggt cggggtgctg ttggggcttt aaccgatgag gtgaaacgcc gtggttatca
    18841 gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc ggcgtggtgg cgaaagtgac
    18901 cgataagatg gatgctgcag ggctggcatg ggcgatttac gacggcgtag tgcccaaccc
    18961 aacaattact gtcgtcaaag aagggctcgg tgtattccag aatagcggcg cggattacct
    19021 gatcgctatt ggtggtggtt ctccacagga tacttgtaaa gcgattggca ttatcagcaa
    19081 caacccggag tttgccgatg tgcgtagcct ggaaggactt tccccgacca ataaacccag
    19141 tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg gcagaagtga ccattaacta
    19201 cgtgatcact gacgaagaaa aacggcgcaa gtttgtttgc gttgatccgc atgatatccc
    19261 gcaggtggcg tttattgacg ctgacatgat ggatggtatg cctccagcgc tgaaagctgc
    19321 gacgggtgtc gatgcgctca ctcatgctat tgaggggtat attacccgtg gcgcgtgggc
    19381 gctaaccgat gcactgcaca ttaaagcgat tgaaatcatt gctggggcgc tgcgaggatc
    19441 ggttgctggt gataaggatg ccggagaaga aatggcgctc gggcagtatg ttgcgggtat
    19501 gggcttctcg aatgttgggt tagggttggt gcatggtatg gcgcatccac tgggcgcgtt
    19561 ttataacact ccacacggtg ttgcaaacgc catcctgcta ccgcatgtca tgcgctataa
    19621 cgctgacttt accggtgaga agtaccgcga tatcgcgcgc gttatgggcg tgaaagtgga
    19681 aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa gcggtgtttg ctctcaaccg
    19741 tgatgtcggt attccgccac atttgcgtga tgttggggta cgcaaggaag acattccggc
    19801 actggcgcag gcggcactgg atgatgtttg taccggtggc aacccgcgtg aagcaacgct
    19861 tgaggatatt gtagagcttt accataccgc ctggtaaatg cgctgatgtg ataatgccgg
    19921 atacgacgtt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    19981 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    20041 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    20101 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    20161 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    20221 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    20281 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    20341 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
    20401 cttcgttgcc gctggttgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    20461 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg acccgctttg taatctggta
    20521 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    20581 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    20641 atccctgaac ggcatgaatg cgacgaataa gattcagtgt atcgacaata agcaaatgaa
    20701 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    20761 ctacggctgg atgagaaatc gggaagaggc ctcgcaaaac gaggcctctg gagagcgatt
    20821 aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg tacttggcgt
    20881 tcatatcttt acctgtttcg tacatggttt cgataacttt atcgaggcag acgcgcggct
    20941 cgctggtacg gcgtagcgcc atacgtgcgg cgttcaccgc ttttactgcc gcaatggcgt
    21001 tacgctcgat gcatggcacc tggacctgtc cggcgaccgg gtcacacgtc agaccgaggt
    21061 tgtgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgca cctaacagtt
    21121 ctgccagacc agccgccgcc attgagcacg ccacaccaac ttcaccctgg cagccgactt
    21181 cagcaccaga aattgacgcg ttcatcttat aaagagaacc aatggcactg gctaccagca
    21241 ggtaacgagc cagtgagtta gcgttcactt cgcggataaa cttgtcgtag tacgccagca
    21301 ctgccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcgt ccgccagcag
    21361 cgttctcttc gttcactgcc aatgcaaaca tgttgatcca gtcaacaacc gccatcgggt
    21421 cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca cggcgtggaa
    21481 cgcgcagttt gccaggcaac acgccttcgg tggaaatacc gcgctcaata ccgccacgca
    21541 tgacttccca tacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc agcgccagct
    21601 catttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt ttttgcagat
    21661 cggctgctga actgtacgga taaggaactt caaccggtgc gctattctgc tggccaaaat
    21721 gctcttcatc aacgataaag ccaccgccaa tagagtagta agtctggctg taaacgactt
    21781 tatcgcccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg ttgtctgcat
    21841 ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt gccagcatca
    21901 ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata tccacggtat
    21961 ccggcaggtt acccgccagg cccataataa tggcgatatc agtgtggtgg cctttaccag
    22021 tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta agcaggttac
    22081 gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg gtatgagaac
    22141 tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga aatacatcgc
    22201 gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg aacagagagt
    22261 agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg ctgatgtgac
    22321 cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc aggaacagga
    22381 tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc gggttcaggg
    22441 tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc agcttgttga
    22501 tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg aagccttcac
    22561 gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg ataatcggag
    22621 ccatccacgc gataaccggt gcgttaaagt ggttagccag gtaagacaga atcgagatgt
    22681 tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag ctgaagacga
    22741 agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc gagcatttct
    22801 gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag atgatcggag
    22861 agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc cacagaccgt
    22921 ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg ttccactgcg
    22981 ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc agaatactca
    23041 tcgctttaac gatcatctgc tcaccaaagc gaacgatggt catcataccc acgatcagga
    23101 tcagcgacag aatcgcacgc ggcggtggcg tcatacccag ctggtgagac atgaagcttt
    23161 caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata gcgaagaagt
    23221 agagcagggt aatcagttta cctgcgccaa taccaaagtg ttcttctaca acctcggtga
    23281 tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg tgagcaaaaa
    23341 acgtcatcgg gaacgcaagg atagccatga tgatcagcgg gatcataccg ccaacaccgg
    23401 cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc
    23461 acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc gtgctggttt
    23521 gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt agccagttca
    23581 gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg cttaatcatt
    23641 taccgccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct atcagtgatc
    23701 ttgatcccaa atacaataat ctacttggaa gatagattat cccaacgctt tactctgtaa
    23761 atcaaaagta aaagagaatt taagggcaat atttgcccat taaacgacat ctgacttctt
    23821 ccggggc
//
LOCUS       DAZUFB010000065        23824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZUFB010000065 DAZUFB010000000
VERSION     DAZUFB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.065"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..842
                     /locus_tag="ACHY3M_003020"
     CDS             <1..842
                     /locus_tag="ACHY3M_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFS4317896.1"
                     /translation="VSSQSTALPPRPLLQATQLPALVMTGKPGQSSVVYLREARQRSV
                     SDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQLYGHMDWNR
                     KILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVSASRVQSTT
                     GTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKGTTLKDGLARWTSSEKCPHG
                     TWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQCLLRVTDR
                     PQE"
     gene            847..1284
                     /gene="pilM"
                     /locus_tag="ACHY3M_003021"
     CDS             847..1284
                     /gene="pilM"
                     /locus_tag="ACHY3M_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFS4317897.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1315..2934
                     /locus_tag="ACHY3M_003022"
     CDS             1315..2934
                     /locus_tag="ACHY3M_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFS4317898.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2955..4250
                     /gene="pilO2"
                     /locus_tag="ACHY3M_003023"
     CDS             2955..4250
                     /gene="pilO2"
                     /locus_tag="ACHY3M_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFS4317899.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYAPAAFSA
                     GDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4240..4692
                     /gene="pilP"
                     /locus_tag="ACHY3M_003024"
     CDS             4240..4692
                     /gene="pilP"
                     /locus_tag="ACHY3M_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFS4317900.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEAGRQQESISAVTTNVQALPSPRLTAIFGVHPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4777..6285
                     /locus_tag="ACHY3M_003025"
     CDS             4777..6285
                     /locus_tag="ACHY3M_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFS4317901.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASSRTKDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6287..7387
                     /locus_tag="ACHY3M_003026"
     CDS             6287..7387
                     /locus_tag="ACHY3M_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFS4317902.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTMKARREMVSSLITLCLYPLLLLSLCVENMYNAHTRLIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWISGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKNSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7461..7997
                     /locus_tag="ACHY3M_003027"
     CDS             7461..7997
                     /locus_tag="ACHY3M_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFS4317903.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            8051..8524
                     /locus_tag="ACHY3M_003028"
     CDS             8051..8524
                     /locus_tag="ACHY3M_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFS4317904.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8543..9178
                     /locus_tag="ACHY3M_003029"
     CDS             8543..9178
                     /locus_tag="ACHY3M_003029"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFS4317905.1"
                     /translation="MFLIILLVISATLTIQLLAIRVAGHYLMQVHTLRLPRHLSLLCV
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDATSCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLYLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAATGTMVCMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9183..10547
                     /gene="pilV"
                     /locus_tag="ACHY3M_003030"
     CDS             9183..10547
                     /gene="pilV"
                     /locus_tag="ACHY3M_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFS4317906.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            11162..12331
                     /locus_tag="ACHY3M_003031"
     CDS             11162..12331
                     /locus_tag="ACHY3M_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4317907.1"
                     /translation="MFQKNKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIASYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTTLLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            12373..12663
                     /locus_tag="ACHY3M_003032"
     CDS             12373..12663
                     /locus_tag="ACHY3M_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317908.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(12755..13075)
                     /locus_tag="ACHY3M_003033"
     CDS             complement(12755..13075)
                     /locus_tag="ACHY3M_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317909.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSAEGAIIYNMI
                     KILS"
     gene            13335..14156
                     /locus_tag="ACHY3M_003034"
     CDS             13335..14156
                     /locus_tag="ACHY3M_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFS4317910.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIILWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            14246..15448
                     /locus_tag="ACHY3M_003035"
     CDS             14246..15448
                     /locus_tag="ACHY3M_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFS4317911.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTISVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            15588..15758
                     /locus_tag="ACHY3M_003036"
     CDS             15588..15758
                     /locus_tag="ACHY3M_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317912.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            15807..16259
                     /locus_tag="ACHY3M_003037"
     CDS             15807..16259
                     /locus_tag="ACHY3M_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFS4317913.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRITEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            16256..17092
                     /locus_tag="ACHY3M_003038"
     CDS             16256..17092
                     /locus_tag="ACHY3M_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFS4317914.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            17076..18224
                     /gene="traJ"
                     /locus_tag="ACHY3M_003039"
     CDS             17076..18224
                     /gene="traJ"
                     /locus_tag="ACHY3M_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFS4317915.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            18221..18511
                     /locus_tag="ACHY3M_003040"
     CDS             18221..18511
                     /locus_tag="ACHY3M_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4317916.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            18575..22591
                     /locus_tag="ACHY3M_003041"
     CDS             18575..22591
                     /locus_tag="ACHY3M_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFS4317917.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINQLPQATEHEYLTRKGILAAPGVRLNNKNELVIPFS
                     NSRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIDVAENARQIWTDSPFVFCADNDHQRKINKGVSSAQKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVYPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQEPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMQPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLDTVAEDLNA
                     DVTVMTTAHHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTANET
                     ARPETASLFSRLRRFLTGGKSRTAEPPVIQENKLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEM
                     VDGASTDDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRA
                     ALEKLRQEKGMAHDAIVTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAET
                     PHPEAPVTAKAEAPRAGTPEQPAAPSSSAGNVAVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQAR
                     ETFRLSGETRDRIRNIWAMTPVSNGITVDNDHPHAGQHILDFPMATFSQVMLQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGR"
     gene            22608..22958
                     /locus_tag="ACHY3M_003042"
     CDS             22608..22958
                     /locus_tag="ACHY3M_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4317918.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            22970..23665
                     /locus_tag="ACHY3M_003043"
     CDS             22970..23665
                     /locus_tag="ACHY3M_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFS4317919.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQSSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            23676..>23824
                     /locus_tag="ACHY3M_003044"
     CDS             23676..>23824
                     /locus_tag="ACHY3M_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFS4317920.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPSWQNARTPQAGGKTGQAPGNA
                     PAGGPP"
BASE COUNT         6199 a         6702 c         5814 g         5109 t
ORIGIN      
        1 cagtcagcag ccagagtacc gcactgcctc cccgcccgct ccttcaggcc acacagctgc
       61 ccgcactggt tatgaccggt aaacccggtc agtcatccgt cgtttattta cgtgaggcac
      121 gtcagcgctc cgtcagtgac tggatacgcc agttgctgcc acccggctgg cagttccggc
      181 aacagaacgc tgccataccg gcactgaaca aacgcctccc cgtctggtcc gttaatgacc
      241 agtggcaccg ggcactgaat actctgctga ccgggcagca actgtacgga catatggact
      301 ggaacaggaa aatactgact gtcacgacca ctgcagcacc gcctgttgac ctcacggcac
      361 cgcagggttc acagaaaaca gcggacacac cacgtaatcc gttcaggagc acaaccggaa
      421 cagcggcaga ccaggcacag gtaccacctg ccgtcagcgc ttcccgggtt cagagcacaa
      481 cagggacatc cggtacatct gcgccccgca caaccacgcc agccccagtg gtcacgaaac
      541 cggtagcgcc accaccacag aaggtcacac cgccacggca gtggaaaatg gaaaaaggga
      601 ccacgctgaa ggacggactg gcccgctgga cgtcgtccga aaaatgcccc cacggcacct
      661 ggacgctgcg ctgggaaacg cccgttaact acagtatcga tgcccctctg gttttcaccg
      721 gcagtttccg ccaggcgctg gaacagctct tcgcactcta caccccggtg gaaaaaccgc
      781 tgtatgcccg gaccagcact gcacaatgtc tgctgcgggt gacagatcgt ccacaggaat
      841 aaccttatgg gctggttcat cgtgttcatc atggtcatca cgaccacagg caatttctgg
      901 ttcaccagtc agctctcccg cagcgaacac cgtcatcagg ccgcagaaaa cacacaacag
      961 gcagcaacgt tcattcgtta tatgaacgcc atcaatgatt acctgcatca gcatccggaa
     1021 cgacggacag ccggagggcg gctgacatcc gctcagctcg ggattcccgg gacggatacc
     1081 gtcagtcata tcatcagcca gcaacgtgtt tttgtctggg ctacagaaac cccgggcctg
     1141 atggccgcgc tcagggaaca gagtaatgac tctgctctgc tcggcagggt ggaaaacggc
     1201 cgactgctgg acacagccgg cagagcgctt tccgtcacac ttccgtcagt gatccccgat
     1261 cacgtcatcc tctggatgaa ttagtcacga atctgaataa atcacggatc tcccatgaat
     1321 aaaaaaattc ttgccattat gattgccgcc gctctgttac agggctgtgc cagttacaga
     1381 cagcttgaga gcacaagaaa ggaagccgtt gcggaacaac aacgggcacg ggaaatgatg
     1441 tctgatctgc gaacccggac gccggttgtt aaggaatcct cacgacaatg gatcaatccc
     1501 cgtccggtca tggataaaaa caccggcaaa accgttcccg gttgtcccat tgtcattcat
     1561 acccgtcaga gcatcaccct tcagcaggtg gttcagcgga tcacggaaag ctgccatatt
     1621 ccggtacgga tcacacctga tgtactgaca tacctgaata catccacaac cggcagcaca
     1681 caacaaatcc agggcgcatt acccccaccg gatgcgagcg gcatggttcc ccttgccgcc
     1741 attggcgcat ctgacacgcc accatcccgt ctgccgggaa ataccgcagc actgtctgaa
     1801 ctgaatgcct ccggcctgac gaccctgctg aatgcggtct cctcccgtct cggtatatcc
     1861 tggcgctatg acggctcgca tatcatcttc tattatctcg agacacagac cttccccatc
     1921 acctatatgg acagtcaggt ggcctataac gccactgtgg tcagcggcac catgtcctca
     1981 aacggctcat caggaggcag tgcatcagat gccatttccg gtgatgccag caatacccag
     2041 accaccaccg tggatatgaa gtctgcgctg tatcaggatg tgaaaaatgc ggtcacatca
     2101 atgctgaccc cgggtatcgg acgcatgttc atgtccacgg gctttatcac cgtcacagat
     2161 aatccacagg ttctggaaac ggtccgggca ttcattgaaa aacgcaatga agagatgaaa
     2221 cgtcaggtgg tgctgaatgt ggaaattctg agtatccgga aaacccgcaa tgagcaggcc
     2281 ggtatcgact ggaacgcagt cttcagcgac agaacgctgg gactgagtct cggcagcaca
     2341 ttcaccagtg cagccagtga tgccatcaca ggtggtgttt ccattgtgga cggtaaactc
     2401 accggttcaa aagccttcct gaaagcattg tcttcccagg gggatgtcag tgtggtgacc
     2461 cagaactctg ccgtcaccaa aaacctgacg ccggtgccaa tgcagatcgc caatcagcaa
     2521 agttacatcg agagtgtgac gacggatacc acagccaatg tcggctcatc cacctctctg
     2581 aatgcggcca ccatcaccac cggttttaac atgacccttc tgccgttcat tctcccggac
     2641 tcacagacac tgcagttgct ctattccatg agtctctccg acaaaccggt tattgagaat
     2701 tatgagagcg gtggaagtaa agcccagctg ccgaatgtgg atctgaaaac catcaatcag
     2761 accgtggatc tgaaatccgg gcagacggtc attatcagtg gcttccagca gtcaggacgt
     2821 cgcagcggaa aacagggtgt aggtacgccg ggattcttcg gtcttggcgg tgggatcaac
     2881 agtgaaaacg acgacaccat tctcgtggtg ctgatcacgc ccaatatcat ctgatttgca
     2941 caaggaggac gatcgtgacg gatatttcac cgacagggcg gcaggtgttt ccgctgacgg
     3001 cagggcgcaa tgtgtttctg gccggtctgg aatggaaaac actgccctca cagtaccggc
     3061 atgcccgcga ctttgcccgg gcacagaaag ccgacctttt cctggcctgt cagtatctca
     3121 gtaatgaaga tgccgatacc cacaccatgg tagccactgt cagtcgccgc atactgcccg
     3181 gaaaaccccg tcagtgcttc tccctcgccc tgctgatcct gccactgctt gaacatggcg
     3241 gctatgccat cacggaactg acgttacccg gagaaacacc gcgctatagc tttgtttccg
     3301 ttgtcgacgg tgttctcgtc agtgacctgg tgggaagcgg ggaagaggtt cgggaagccc
     3361 gggatacctt tctttccatc aacaccgaac caactcaggg ctggatacac tatgcaccgg
     3421 cggcattttc agcaggcgat cagaatcagc acctgccgct ggcctccctg actggctctg
     3481 gtaaacatcc ggctgccgca cgcctgaatc cggtatcccg gggaccgcag ttcatcactg
     3541 tctctctgat catcgccctt ctcggcactg catggtacgg ctggcagtat tatgaacgct
     3601 ggcagacaga aaaagccgcg caggcagcgg ttgcccgaca ggccacagag gaacgaatca
     3661 ctcccccctg gcccggactc cctgaaacag acgcgttcat ccggggatgt gctgatatat
     3721 ggacttcact gccactgtct gctgccggat ggcgttattc gctggcagaa tgctcgactg
     3781 acggtggcag cggcaatctt cgcgcatcgt acaccaatac tgcgggcgcg acagtgactg
     3841 acttcatccg gcgcatcagt gatatgacag acagccgacc gtttattgtg atgccggaag
     3901 gaaagaccgg cggcattggc ctgccggtca ccttccggct gtctgaaaac gccgtcacag
     3961 ttgatgcact accggaaacc gctgtcctgc aggaacgact gacctcgctg gctcagtcaa
     4021 tgcaactgaa actggactgg caggaagtta ataacagcct cactgatgag aacggacaga
     4081 tcattcagcc cccatggaaa gagtatgacc ttcagatgca gaccacactc cctgcacacc
     4141 atctggctga acgcctgaaa gagccttctg tccgttttat ctctgtcaca cggcaactgg
     4201 agaacggtcg tttccactat caatttacag gtaaatatta tgcccgataa agccaaaacc
     4261 cttctgtttt cagtcctgct gttaccggcc acggcaatgg ccacaaccgg aaatgcagag
     4321 caacagaaca cacagcaaat aaggattgaa cagatacagg aagagacagc cctgtatgaa
     4381 gctatgctgg cgagagagaa gatccgccat gaactgcgaa cacttgagca gggcgttccc
     4441 gcagaggccg gacgtcagca ggaaagcatt tccgcagtta caacaaatgt acaggcatta
     4501 ccatcccccc gcctgacagc aatattcggt gttcatcctc agctgactgc ccggatagaa
     4561 cttccggggg gaagcatctc cgatgtgaag gcgggccaga acatccccgg aaccacttat
     4621 cacgtggaaa aaatcacgcc ggacagtgtg gtgctggaac agtctggcac cagaaaaacc
     4681 ctgcgaccat gagaccggcc gccgtaactg gcggctgtca aaaataaaag gatgaaagac
     4741 atattttccg ggcaccgctg cccgtaagga ggctttatgc tggatccagc agacctgccg
     4801 ccggatgaca ttctggatta tgtggccatc gacaccgata acgccggaca actgcggatc
     4861 agggttgttg agggaaaaaa acatctgcgt gccgtccagg aatatctgac ccgccttcgc
     4921 gcacgctatc aggggcgtgt gggagacttt gagttcacca cgctggatgt gatagcccgc
     4981 ctcagacagg acgccacaac gacaggcgat gaaaacgcta ttaacccgat acagcagaaa
     5041 atgctcggct atatccgcca ttctgcctcc cttggtgcct ccgaccttca tatgacgcca
     5101 gggcgggaca acacagactt cacctatgtg gaagcccggg ttcatggcga actggaagtg
     5161 ctcgatatcc tccggaaaga agaaggtctg gaattactgg gggctaccta ttccggcatg
     5221 acggatgtca tcaagggcac acaatttgat cccggggttc cacaggacgc acgtctctct
     5281 gaacagtacc tgaaactggc tggcctgttt ggcgcacgct actcccacta cccctgcgtg
     5341 ggaggcctct atgctgttct ccgtctgatc aaggatgaca gccagcaaat cccgaccttc
     5401 agtatgctgg gttatcaccc ggatcaggag tgcaccctgc gccgtatcct gcaacgccct
     5461 gaaggcatta tcaccctctc tggccccacc ggctcgggaa aatccaccac gctgagaacc
     5521 gcctctgcgg cataccttga acaatacggc ttcaacaaca ccggcggtat tcttctgcca
     5581 cggcgcaggt tgttcacgat tgaatccccg ccggaaggcc gtatccccgg agcaattcag
     5641 acagccgtta tggataccac ccggggctgg gtggactcca tcaagtcagc actgcgtctt
     5701 gatcctgaca gcatcctgaa cggtgaaatc cgcgatcacg attccgccat tacggcaata
     5761 aaagccgcca tgaccgggca tctgatgctg accaccatcc atgcgaacga cccgattaac
     5821 atcctcgaac gccttgagat ggagggtgtt caggccagga tgattgcaga cccgcagttg
     5881 ttcatcggac tgctcagtca gcgtctggtt cagctgatat gtccccactg tcgtcatcca
     5941 tggaatgacg tagcatccag tcgtacaaag gatgagcgac atctggtgga aaccttctgt
     6001 aatgctgatg cggtatatct gagaaaccat gaaggctgtc cacactgctg gcgtggtgtc
     6061 acagggcgta cagtgattgc agaagtcatc tctcctgacg ccagattttt tcagttatac
     6121 cgggaaaaag gccgtatcga agccaaaacc tactggcaca gggaactggg cggaatgacg
     6181 cgtaatcagc atctgctggg aaaaatcaac agcggactgg ttgaccctct ggcggcccat
     6241 tacatctctc ccattgatga ggacagctat acgctgctgc gctgatatga aatttctgga
     6301 aaaactcaac agtactgaac tggatctgtc tgccctgaac agctggacag ccagaaaaac
     6361 cttcggtctt aagcgtgccg agttctatga gtccctcggc ggaatgatac gtgacggaac
     6421 gccgtcactg cgcgccctgg agtttatctg taatgtcgaa actgactttg gcacccgaaa
     6481 aggaacatcc ggcatgtact ttctggccag agaatgtatt gcctccctga aagccagcgg
     6541 tatgctgtcg ggggccctgc ccacatgggt tcccccggaa gaagcggcac tgatcaggaa
     6601 cggtgaagaa cgcgggaaca tcgccgaagc cctgttccag gtcgcaaaaa ccatgaaagc
     6661 acggcgggaa atggtgagca gtctcatcac actctgtctt tatccccttc tgctgctttc
     6721 actctgtgtg gagaatatgt acaacgcaca tacccgcctc atccccattg tcagcgcttt
     6781 tgcaccggag gagaaatggt cattccagat gaaagtcctt gcagccatgt cagagcttgt
     6841 catcggctat ggtctgtgga tcagtggtgc catcatcacg cttgtcatca tcatccgctt
     6901 ctcactcagt cgctataccg gcccgggacg caggcttctt gacaaactgc caccatggtc
     6961 gctctatcag acttttcatg gtgtgaatta tctgttcagc atatcgtcaa tgctgcagat
     7021 aaatattccg ctttcacagg cgctgctcag aattgaaaaa agtgcagagc ataataaatg
     7081 gttaaaggag agaattcagg caataagaaa aaatgtatta tccggtcaga atctggccac
     7141 cgccatgcgc aattctgact atgattttcc ttcaaaaata tgcatcaata aacttctctt
     7201 aaattctgaa agggaagaca gcgtatctgc aatgaatacc tatgccggac agtggctgga
     7261 agatgcaaaa aatagcgtca gaaaaagtgg attaattatc accggtattt caggaggttt
     7321 tgtattcttt tttattctta acatgattag cgcgatatac agtatcagcg atatgctcaa
     7381 gcaataatac ctgccgtcac actaaaacgc ccttcaatat actttcatta acgtaataac
     7441 cattaataca ggagtcatat atgaacttca gagcacgtct gtcttcttat ttttctttaa
     7501 aaaaagataa agaagatgat aaaggtttaa cattattaga aattgccggc gcaatgatta
     7561 tcatcgcaat agttgtcgcg ctcgcgatga gccagatgtc cagcacaatg gcctccagtg
     7621 acaaccagcg cgaactgaaa aacctgcaga caattgcaac caaaatgaag caatacaagt
     7681 tccagggaag atataccgga acggattata tcaaaaccct gacggaaagc ggtgcattgc
     7741 ctgctgatat gattgcagga ggcaacaaag ccaaaaacgc atggggtggt gatgtcacga
     7801 ttgctgccac agcagacaaa tatggctaca ttatcaccag taataatgtt cctaaaacaa
     7861 actgtgttga gctgattaac agcctgcgca gttcgtccat atttaccaaa attatgaaca
     7921 cagccccgaa cacggtggat cccgttaccg tctgcagtag cgataaaaac aatatcaagc
     7981 tggaaaccaa cagctaatgc cctgccacac agcactccct ccggggtgct gtttcccgga
     8041 gcacataatc atgaaatggt tttatctctc atctcttttc ttattctgtc tcccggcgac
     8101 tgccgccgta aacagtaaca cactggatag ctgctttatt caggcaggaa aacgttactc
     8161 catcgatccc ggactactga aagtaattgc ccgtaaagaa agcagcctga atccccgggc
     8221 catcaatcat aacagaaatg ccagcggaaa aattatcagc acagattatg gcctgatgca
     8281 gattaattcc acacatattc ccggactgaa aagaatggga attatccgaa gtgaaaatga
     8341 actgctcagt aatgtttgcc tgaatataca gacaggtgca tggatccttg cccggcattt
     8401 tcagcagtgt ggtgtgaact gggagtgtct cggtagctac aacgccggat ttaataaaaa
     8461 taacactcac cgcagaatga aatatgcccg gctgatttat gccgcatata tgcagggacg
     8521 ttaataacgg agaatacgag ccatgttcct catcatctta ctcgtgatct ccgctacgct
     8581 gaccatacag ttacttgcca tcagagtggc cggacattat ctcatgcagg tgcataccct
     8641 ccggcttcca cgtcacctct ccctgctctg tgttgttctg gcactcaccg ccaccattac
     8701 gcacatcatc cgggggacag acattaccgg actttgcttt ctgttgctga tggcaggctg
     8761 catgacactg atgacactaa ctgacgcaac ctcctgccgt cttccccgtc ccttcaccct
     8821 cacattcctg atgctgggtg tggcgtttcg tttatggcag ggcgatgctg ctattgcgct
     8881 tgcctgtgct ctgttctggt ttctcctgtt atatctcctg aggcgctaca cctcataccg
     8941 caagggagtg gagacgctgg gactggggga cgtgtttctg attgcaggtg tggccatgtg
     9001 gtcatctgcc aacagcactc cgtggatcat cggtattgcc gccaccggca ccatggtctg
     9061 tatgtcgctg ttcccgcgca gtaatacagg ggccgctatc ccatttggtc cgtttatctg
     9121 tgcatcactg tacattttca ctttttttcc tggtgccatt tttcacaccg gagggtaaca
     9181 ccatgcaaaa agacgacgat aaaggttttg ttctgtttga aattctggcc ggactgattg
     9241 tgatcggtat cgccacacca atgatttatt cagaaattga aaactggctg aatgagcagc
     9301 tttaccagag tgctgcatat catgcagatg cctacaatac cgcagccaga aactatattg
     9361 ccgacaacaa tgcccgtctg catagcggca gccttccggc aaatttcacg gctgatgatc
     9421 tgattcgtca gggatatctg aaacaggggt ttaaccattc tccgtttggt cagagttata
     9481 tcaccggtat ccgccgtaac cagaccaccg ggcgtctgga agccctgacc tgctcaactg
     9541 gagggcagac aatcaaagaa gaagggctgc gtagtgtggc gggtcagttg ccggggctgg
     9601 gtggatttat cagcaaaaac ggaacggcaa caggcgcatt cggagcctgg acagataaac
     9661 cgggagacta cggacttacc tgttcgacag gtcacatcgc cgtcgtcatg agcggtgatg
     9721 acctgcagga aagtgaccgt ctctaccgtt ttcaggtagc aggtcgtcct gaactgaatc
     9781 agatgcatac tgcaataaat atgggcggga ataacataaa caacaccggc aatatcaatg
     9841 gtcagagtgc cacactgaaa ggtgatatta cgagtgaaga cggctggcta atcaccagaa
     9901 acaataaggg atggatgaat atcacacatg gtggtggatt caccatgacg gacagccagt
     9961 ggatacgcgc ggttaatggc aaaggcatca caaccaccgg ggaaatcaaa ggggggaaag
    10021 tcagtggtgg tacagtccgg tctgacggtc gtctttcaac cggggagtat ttacagcttg
    10081 ataaaacagc tacggcaggc acaaagtgtt caccggacgg acttgtaggc cgcaccagta
    10141 caggggccat actttcgtgc caatccggta tgtgggcagg ttttgaatcc taccctgtcg
    10201 gaagccctat accgtggcca tcagccaccc ctccacaagg ctacctggtg atgaacgggc
    10261 agtcattttc atgttcccgg tatcctcagc ttgcccgtgc ttatcccggg tgtaagctgc
    10321 cggatttgcg cggtgttttt atccgtggct gggacaacgg acgtgggctg gatggagatc
    10381 ggaacagaca tctcctgagt tatcaggccg atcaatccgg tgtctatcat gagcgtgggg
    10441 gatggctgaa agggcatcac agcggtatgc cttactgggc ccagggaagc acaacggaaa
    10501 tgcgccccaa aaacattgcc tttaactaca tcgtcaaagc aagctgatgc atttaaggcg
    10561 tattaacttt taacttacag caacgtattt tgacccagac catgcttccc gaataccccg
    10621 gtttacctct gtacgctctg aatcctgccg caccatagcc tgctttgccg tcacagacgg
    10681 caaagccgtt tgagccacta ccccagttcg cataaaccgg ttttctggag tcttcttctg
    10741 cgtcataaca ggccgaataa tcaaaaacca gtgcatcgct gatattattc ttccagacac
    10801 cggattggca cgctggctac tgcctgacgc ttaattaaat accggcccca ggtatattga
    10861 gacacgtaac agatatcgtt ccacctgact cataattacc ccacgcataa ttattgacat
    10921 accagacatt atttttatcg gaataaacct gctgaaaacc accgaaagga gaaccattaa
    10981 gatacgtcca tgcacaatat gcatggacac caagtctgaa attacgaata ttttttccga
    11041 tgttataggt ctgcgttgtg aatttaattt cagttgaccg ccagacaccg gattggcacg
    11101 aaagtattgc atttctgtat ccagcctcct accttccccg tttttatcgt ggaaggcaga
    11161 tatgtttcaa aaaaacaaaa ctcgcaaaat gtccctcagc cgggcactgg acaaatacct
    11221 gaagactgtt tccgtccata aaaaaggtca tctgcaggag ttttatcgcg tcaatgtgat
    11281 taaacgtcac cctatggccg atcgctatat ggatgaaatc actaccattg atatcgccag
    11341 ttaccgggat cagcgtttag ctcaaattaa cccacgaaca gggcgtcaaa tcaccggaaa
    11401 taccgtccgt cttgaacttg cccttttatc atcattattc aatatcgccc gtgtggaatg
    11461 gggaacatgc cgtatgaatc ctgttgaact ggtccgaaaa ccgaaaatca gtaatggccg
    11521 cgaccgccga ctgacttctg gtgaagaacg ccgcctttcc cgctatttca gggacaaaaa
    11581 tccgcagctc tacgttatct tccatctggc tctggaaaca gcaatgcgcc agggagaaat
    11641 tctctcatta cgctgggaac acctcgattt gcagcacggc gtggcgcatc ttcctgaaac
    11701 gaaaaatggc tcaccacgcg atgtcccact ctcccggaaa gcacgtaact acctgcaaat
    11761 tctgccacaa cagataaacg gcaatgtgtt cagttacacc tcgtccggtt tcaaaagcgc
    11821 ctggcgtacc actcttctgg atctgaaaat agaaaatctg cattttcacg atctccgcca
    11881 tgaggccatc agccgtttgt tcgagctcgg cacactgaac gttatggaga ttgcagccat
    11941 ttccggtcac cgctccctga acatgttgaa acggtacaca catctccggg catatcacct
    12001 ggtcagtaaa ctcgatgcaa ggcgtaaaca gaccagcaaa atttcccctt attttgttcc
    12061 ctaccctgca actgtcagaa acagaaacgg agtgttcatt gtgacactgc acgatttcga
    12121 ccttgaaagc cgtgctgaaa cccgggaact ggcaatatct cacgccagcg tattgttgct
    12181 gcgcgcactg gcccaggctg ctcagcgggg tgaacgcgtc cccacaccag gcgagctacc
    12241 cgcaaacatt gatgaacggg tcatgatttg tccgttaaca aacagccagt tcatgacaaa
    12301 agcgaacagt attttcatat cctcctcctg aacaaataat ttttgttcaa aattaacgct
    12361 atgagaccct ttatgaaaca cttatcctcc cgtgcagcca ctgcggataa taatcgtatc
    12421 gtaacaattg aagacgttta cacaatcgca ttctcgcaac caattcacac cgaatcagcg
    12481 ttggggcaaa ggtggatgtt gatccatact ggtgaatatg aaagtgccag cgatgcaaaa
    12541 cgcttccttg atacgctcgt caccgggcac tgtgcttgca tggtaacacc atcacctgtt
    12601 ctggatcatg acacctggct gcgtatccgc ctgctgatgg gcgagataag ggatgccaaa
    12661 taaacattca tggccccaac acataaaaat aacgccgggg actctggcag aggtaccatt
    12721 ctttttacat agagccaata tataaacaac ttttctatga taatatcttt atcatgttat
    12781 aaataattgc cccctcagca gaaagggccc aaatagaaaa agcaaaaatc cttataaaaa
    12841 tattctttac aagcgttcct atatacaaac aaactatata aaccagccag aacagaatga
    12901 ctgcaaaaga aacacacacg gcccccataa aaataaatgt catcgaatcc catggtttca
    12961 ccagtacaac cggaaccatg gctgccagaa caataagagc cgcattaccc aacagcgtct
    13021 ctcgaataat atcccttata atctccagtt tttctttatg ctgtttaatt ttcatacgaa
    13081 cctcgttgat ttcatcccga aaaggaaaag attatataac gaaatacttt caatctcctg
    13141 actccgtctg ctctgcagca taatgccaga ccagcggcat gctaaatcac gtcctgtccg
    13201 aatccttttc attcacgctt aaaacagagt taaaagcact cttgaaacac actcgatcat
    13261 aacgatcgaa ataactcgtt tccgttcagt aaacacgctc tttagactta acatatgaac
    13321 cgcacggagg tgaaatgaaa cccaacagca caggcattgc tgcccgcatg atgctctcca
    13381 ttgacagggc ccggattagc gaagctctgc tcaataaccg ccagttgcag tccactcccc
    13441 tgcagatccg ttatccgcag ggcgtcagag aagcactcgg aataatgagc gagcaactct
    13501 ccctttctgt atccgacctg acgcgtatcc tggtcgaaga tgcattaact gaaatgtttc
    13561 tcccggctga aaacatcgtt caccgtctta tctgccggac agaacacatc atgcaggcac
    13621 atgatgtctg cgtaacaacg atgacaacgt tactggctcc ctggaatatc cgtcctgccg
    13681 ttttccgtga cccggacaaa ctcgcagatt atctgacagg agagattctg acggctctgg
    13741 ctgactggtt ttatctcaac ccggaatggc tgaacggcag ggtacgttat cctttaagcc
    13801 gtgccggtga gtggccagca acacaggatg cgttctgcca gcttatttca gccagcgata
    13861 atgtggatat tattctctgg cacagttttc cttttgccgg tgttcattca caggaatatt
    13921 gcggtgtact gttccgcaag aaaaaagaaa ttaataaagc catcatttat cctgtacttt
    13981 ctttatcacc tgtaagaatg aataaggaaa aagaaaactg gtttcacact gcccgcaatt
    14041 tatatgcaaa gacatccgtc cggaccgtaa cgcttcgtcc ggcacaggca gaatatctga
    14101 tgacaggaaa aatattacct gctgagttat tcagtatacc gttattaccc tggtaataac
    14161 gcagagaaaa aaccaataaa tttttaatgg cacagtcgtg tcataccgtt gctgtaccca
    14221 aaacacctca aaccaggaaa ctgaaatgat gatgaatatg cacattaccc gaactctcat
    14281 cgcatctgct gttttatttt cctttaattc aacagccgca acttcatatt ttgaagcccg
    14341 caatgatgca atggggggaa ccggggtagc ctcctctcac tacggtgtgg cacctctggc
    14401 aaacccggcc ctgctgacaa aagccggtcc ggaagatgac ttcagtcttc tgcttccgtc
    14461 cgttggtggg caattgtccg atccgggtaa tatcagtgac aacgcagaca aaatcagtga
    14521 tgactggaaa gcctttgatc gtgccatcga cagtcagagc aacgtgtcgc agactgctgc
    14581 acgtctgaag acgcgacttc aggacttccg tcacacacat gctgacgcac aggtgggaac
    14641 atctgctgtg gcgactcttc ccggtgacac cctcgctgcc gctctgatgc tgaaaagcta
    14701 cggtacgatc agtgtcgacg gtaaagtcag tgatgccgac ctgaactatc tggaaagcat
    14761 tgcaaacagt aacggtcagg atgtggataa agacagactg acctctcagg cttttgcccg
    14821 tgccgcactg gtcaccgatg tgggtattgc tctggctact gaactggaaa cggccggaca
    14881 aaagtggtct ctgggcttca caccaaaatt ccagcgtgtt gacctgttta actacaacgt
    14941 gctggttaaa aattacgaca gcagcgcttt caaaggcgac cgttaccaca acaccagaaa
    15001 cggcattaac gctgatatcg gggccagcat ggacctggat gacaactgga cactgggtct
    15061 ggtcgcacag aacctgctcc cccgcagtat tgagacaaag gaagtgaacg gtgtcacaga
    15121 aactttcagg atccgcccgc aggtgactgc cggtgtctcc tggcacaacg acatgttcac
    15181 tacagcactg gatgttgacc tgaccccggc cagtggcttc acatcagaca gtaaacgtca
    15241 gtttgctgcc attggtgctg aatttaatgc ctggaaatgg gcacagttac gtgcaggcta
    15301 ccgccagaat ctggccagca atgatggcag tgcgttcacc gccgggctgg gtatctcacc
    15361 atttgatgtc gtccatattg atgtggcggg tatagcaggc acagacaaca actacggtgc
    15421 cgttgcacaa ctgcagttca cattctgatc atctctgccc cggtactgac cggggtatcc
    15481 agacctgcat cctcgcgttt tccgaccatc aaacgacaca gccagacctg tagtgctgcc
    15541 tctgatctgg ctgttatctg acatatcatt ttcgcatgag gaaatgcatg tttaacaaaa
    15601 ataaaacgca tatcgaaaac aaagccttta tggtggaacc gttccgggat ccgacagcta
    15661 acgaatgtaa tgtcgtgata gcaaacgata tttgtatccg ggtagcactg tctccctctg
    15721 aggtgctcca gccattcagc aaaaacacga accagtaaat cctccccaca acgctttctt
    15781 ctttcactgc tcaggaaaaa tacaccatga accgtatgat acctgcggga tgtctcgccg
    15841 tactctgcct gaacggctgc catctccccc cggataatac agctccgacc agaaagtatg
    15901 gtgctgcctc tgatgtcgcc gtcgcccggc atgcgcagta tcagctatgg gggcagggaa
    15961 tttactctgc agcccttccg gagcgtcagt ccggcggcag tctgaaggct cacagcgacc
    16021 gtgtttcctt tgactgggaa ggcgatgccc tggaattgct caacgaactg gcccgtctgc
    16081 gcggccagca gttcagctac agcggcgtcc gtctcccctt acccgtctca ctgcacgtcc
    16141 gcgacatgac ctttgctgat gtccttcgta tcactgaagc ccagactgcc tggcgggcaa
    16201 cactctcgca gtcagccggg cgtttacatc tgtcttttat gacgccggag aaaaaatgaa
    16261 aacacacctg tgtctcctgc tgtctgctct cctgcttaca ggctgtgccg gacagcatca
    16321 ccgttcttct gccgtcatga cagaaaacgc accgcccccg gatatccgtg catggctgac
    16381 gccctccgga aaccaggatg atggtatttc agatacccgt cgtcagatgc tcacggatgc
    16441 cggcagaacg ctgggattcc gtggtggaaa agcacagcgc tcatgggaac tgattcaggc
    16501 actgaatgcc cgggaaagta ccctgaatgc cctgtatgat ttccggcctc tcatcagcaa
    16561 ggaaggctgg ctgcctcctg ttatcgatga agcgcaggat gttgcacaca tcagaccgga
    16621 ccagataaga acagcctccc gtgtctggac catcctccgc ccggaacgct ttgtcagtaa
    16681 tccacctgga tggcgtgact ggctgctgaa aggactctca accaccgcca caccaggcac
    16741 tgaaggccgg gttgtgccgg aagaccgggc acaacgcaga ctatgggaaa acgccttacg
    16801 tcagggatgg caggaagggc gcctcaacgc agatctcaca ctggaagcta atcagaagcg
    16861 cctgacccgt gattaccggg gcatgatgct ttacgccctt ctgtggcgtc agggcatgat
    16921 cacacgtcca gatgtcacag agcagatgca gacggttacc ggcaacgggc aaaaactcat
    16981 cactggcgat cgcgtccgcc gcctgaaaac acatgctgaa tttacgctgc agaaatcgcg
    17041 ctggcgccct cttgtcagca ccgaaggaga gaaaaatgat atcaccagat gacttcggta
    17101 ccttcccctt ctcccgtttt acggcagaag agtttcgcca tttttttgcc tggtgtgccc
    17161 gccaccgcgt cagtgatatt gatctgaccg gcggttcacc ggtatccgtc agtcgctttg
    17221 gacggcgggt tcgctcatcc gtaaatcccc ttccgtcttc catcatgatg gcactgagtg
    17281 atgagctgtt tgggcgggag gttcttcccc gggttctgtc aggtcatccc gtggatcgta
    17341 ccattcaggt cagcggagat gcctccggcc gctatggcct gcagcgaggt cagcgtgttc
    17401 gcctgcgctg ccacctgata cagggaacat cggcaacaga agaaaaagcc atttccgtca
    17461 ccatgcgggt gatcccgtca gatattccgg acatcctcac tatgaatatt gaaccggatc
    17521 tgctggatgc catgctcaga aaaaccggcc tgggcttcat ctgcggagaa accggctccg
    17581 ggaaatccac actggcggcg gcactgtatc gccacattct gaaaaatttc cccgatcgca
    17641 aaatagtgac ctatgaagat ccggtggaat acattctggg cggagagaat gaccttctgc
    17701 ctccccatca ggccgaaatc gggcgcgatg tggaaagttt tgctgcagga cttcgctctg
    17761 ccgttcgccg taatccggac atcattggta tcggtgaaat cagggattat gaaacagcag
    17821 atgccgcagt ccaggcgggt aataccggac acttttgtat cggcaccatg cacacaaaat
    17881 cacccggaga aacctttgcc cgcctgctgg gattgtttcc tcctgaaatc agggattcga
    17941 tggctgcagc cacgctttcc ctcgtacagt tcattctggt gcaggtactg gtcagaacaa
    18001 atgacggcgg caggcaggct gtccgggaat acatcgtcat aactgacgaa ctgcgcaata
    18061 cgctctccgg acaatcccat gcaacatggg gacattacat tgacgaaatc attcgtcagg
    18121 aaaaacggcg gataagggat caggttctga ccatgtatca gaacgaccgc attgagaaat
    18181 cagaagcagc gctcttcatc ccggccggag agttcccccg atgaaacgcg aaggcttctg
    18241 gcgggatgta acaccaccgc tcaacattta tggtgtcccc ctgccgttta ccatgattta
    18301 cctgctgttg tttccgtatc cgtcaaaaac gacattctgg gtgtgcagcg gcattctgct
    18361 gttttttatg ctcctgggat attccggctg gacagtccgc accctctgta tacgtctgta
    18421 cagcctgctt cagggtaagc aggcatcggg acgtccctgg tggtaccgac accgcacaga
    18481 attaccccgt gaatggcacg gaccagaata gccctctttt gtccatcatc ccggcgtatc
    18541 acaacgccct catatttaat ccccggagaa cgacatgaag tccagatatc gtacctggct
    18601 tgctgtcccg cctgaggaga cagaagccgt aaaaaatgca gtccccccgt taaatggccg
    18661 caaagctgtc gcatgggatc cagaaaaaaa actctggtat gcccgggcgg gtaccgaact
    18721 ttcactgctg gagcgctggt taccccggcc gcaggaactg tcgatggatg ccggcgatcc
    18781 ggtcaccgaa tttgctcagg tcctggaaaa cgccggactg gttattcagg gattgccaca
    18841 gatggatggc gccatacaca gagtcgccac ccgggatgac aaaaaagggg ccaaaagtgg
    18901 cgcatacaag gcctatcttg atgggcgccc cgccggctgg taccgcgact accggagcgc
    18961 cgatgacagt cccacaaact gggtattttc cggcggagaa caacacgatc ctctggccag
    19021 actgcatctg cgggcattcg ctcagcaaca gcgcgatgat aacgcacgta aactgcagca
    19081 acagtacaac aaacaggccc ggtatgcccg ctcctatatt aaccagttac cacaggcaac
    19141 agagcatgaa tacctgaccc gaaaagggat ccttgccgca ccgggcgtcc gtctgaataa
    19201 caagaatgaa ctcgtcatcc ctttcagtaa cagccgggga gaaatacgtt cataccagcg
    19261 tattcctgtc accggcggga aggatgcccg cattctgaaa gactcagaga aaaccggtaa
    19321 ctggttcacc ttcggaaccc cggaaaatgg ccgtcccctg ttatttgcgg aaggttacgc
    19381 aactgcagcc tccctgcacg aagccaccgg ccttccggtt ctgatgacag ttgatgccgg
    19441 caatatgatt gacgtggcag aaaatgcccg ccaaatctgg actgacagcc cctttgtctt
    19501 ctgtgccgac aacgaccatc aacggaaaat taacaaaggt gtttcaagtg cccaaaaagc
    19561 tgccgcaagc acgcatggcg aagtcattat tccggccttt acggaggcgg aaaaagcaca
    19621 gggactgacc gatttcaatg acctggacgc cagcagggga cgagacgcct ttcagcgagc
    19681 catgaacgca cagttagaat atatcggtat ccccacgccc aacaatgaca ctactgaccg
    19741 ccgtgaagct atcgtgatcg gcaacctgat attcacaccg gtaaaagatg agaagccaca
    19801 agtgtaccct gaaaacagac agagcacagc ccctgaaccg gcagtgtcgg aacagaatca
    19861 tccttatgac atccctgcag aagagacaga caatcaggca tatgaagatg actatgcagc
    19921 atatgcatcc ctgcagtcgg taactgcagg gacagcctcc ccgaatgaac cgcaggtgaa
    19981 tgctgtttct gccattgaca gcaacgcaaa gaatacctct gccgccacca ctgacgaccg
    20041 aaatgtatta gcacaagaac ccaatgctga cgccacagtt acgaccacgg ctcacgacac
    20101 cggcacagca gaaacagatt caccttctga acctgcagag attcagccag tgcagcaggc
    20161 cagtcctgaa ccatcggcta aagataaacc acaggagaac gtagtgtctg ctactgataa
    20221 tgacgcagag aacacctctt ccgccaccac tgccctccgg agttacgaca caccggaaac
    20281 atccgtctcc gacgcaaccg ggacacagca caccgccaca acgcaacctg aagaaccaca
    20341 gcaggtctcc atgggcagcg ctgacgactg gagcgcattt gcggaagacc tcaatgctga
    20401 tgctccgggg aatgccactg ttcacagcac cgacaacgcg gagacaaaga cactttctga
    20461 acctgccggg atgcagcctg ttcaacaggc aacccctgaa ccaacggaag aaaataaacc
    20521 tcaggaagag ggcgttatgt ctgccactga tacaggtgaa gagagtgccg ctgccgctat
    20581 cgatgacgac ctggacacag ttgcggaaga cctcaatgct gatgttactg tgatgaccac
    20641 agctcaccac accgatacag cagaaacagc ctcaccttct gaacctgcag aaattcagcc
    20701 cgtacagcag gcaacccctg aaccaacggc agaagataaa ccgaaggaag agcgtccagt
    20761 gtctgccact gagaccagca cagcgaatga aacagcccgc cctgaaacag catcgctttt
    20821 cagccgcctg cgccgttttc tgaccggtgg aaaatcacgt accgccgaac ctcccgttat
    20881 tcaggagaac aagcttacgg aggccgaaac tgcgacagag gccgcagcac caccgtcacc
    20941 acctgaaacc agcccggaag acacatcgtc agcagaaccg gacacgatta tctttgcgcc
    21001 ccgccggccc aacagtccgc agcggcacaa cctggacgag attattaaaa atcttacata
    21061 cgatgagtta cccgacaata ctgcgctctg ccgcgtcgac ggcgaaccgg cattcctgga
    21121 taaactcaac cgtctggaaa tggtggatgg tgccagcact gatgacaaaa aggtcctggc
    21181 tgcccttgct gtggccacca acttctatgg cggcgttatc gagctgaccg gcagtgatgc
    21241 ctttaaacaa aaagccatgc agctgattgt cgaatatgac atcaaggttc gcatgaaact
    21301 cccggtccag agggctgcgc tggaaaaact ccgccaggag aaagggatgg cgcatgatgc
    21361 catcgtcacc cacagaccca cgccggaact caaccgcctg tcaccggaag agacaacaat
    21421 ggcacaaccg gccccccctg tgtcaccgaa agcagaaaca gagcaaactg caggcagtac
    21481 ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa gctgaagccc cccgggccgg
    21541 cacaccggaa caaccagccg ccccgtcatc gtcagcagga aacgtcgctg tcagtgcaca
    21601 gccacagcaa cccgacacac cggaggaaga gccccccggc aaactcagac ccggtgaaag
    21661 tgtgactgcc gtcctgacaa actacggaga aaaagagtat gcgcccggaa aagggcgctg
    21721 cttttttgtg gagctcacca accgcagtgg caaacgggaa tactggggaa acggcctgga
    21781 agaactggtc aaaaaccacc agaaaggcaa ccccgtcaca ctgacgctgc aggcgagaga
    21841 aacattccgg ctgtccggtg aaacacggga tcgtatccgt aatatctggg ccatgactcc
    21901 tgtcagtaac ggcattaccg tcgataatga ccatcctcat gctggtcagc atatcctgga
    21961 ttttccgatg gcaacattca gccaggtgat gttacagatg caacaacact ggccacagat
    22021 aatgcaggaa gttcgctccc ccaaaaatcc tcccaataat ctgtacattg gtgaagacag
    22081 gcaacctgca tcagcacctc aggatcaatc aatgcgactc cccctcgctg gtaatgcccc
    22141 caatgaactg ataccagcca tcgcctctgc cgataaggac aatcgtcagt tgaatcttct
    22201 gctggtccac agcgcagatg atcatctgca gggcgtggtc cgcctgaacg gcacccttta
    22261 cccggcactg gccaccccgt cagctgacaa ccgtcagctg gtcatcaatg ccctgacgga
    22321 taacggtctg cagtttgccg gttacggtga agctgttaac catgatgaga ataatcatca
    22381 gcgtccgtcc ccacaaataa tgcagtttca cctgaagcaa caggactccc cgttgtttgc
    22441 cgccatccac aaaccggaag agcaaccgga caagcttttc cgctctctgg gttttgagca
    22501 gacctggaaa gagtggtctg acagccaaaa ggcggaggac aggcaggaaa aaactctgca
    22561 acaggcacaa tcccacagcc ccggccgatg agccggtaag gacgaccatg aacatgaaaa
    22621 aaacgctgct ggtcatactg accggcatca ccctggcatc accggtggca ggtgccgcca
    22681 cctgccgggc gggggccgct gccgaagccg gcagcaaagc agcctgggag cgggcgcgca
    22741 cagccagcca ggcatggtca cagcgagaaa acgacgtcgc ctcctccctg cagtcctgcc
    22801 tgtcccgcat taaggacgtc agcatcactc ttccgcaatt ccccagtctg gacaaaatcc
    22861 ttcgcgatct ggaaaaccag gtctgtgatg ccgtcgtgga tcgggtgaat gaacatctgc
    22921 cggggaatat cgacccctgg aaagactata acctctgaac aggagcccca tgagcgaagc
    22981 acaccccaca tcccctgtct ctccggaaac agataacagc ggagatattt ataaacaaac
    23041 gctcagtacc attgcacagt cagaacaacg ggccgcactg gttcccggac ttgtcagggc
    23101 cttgctgtgt agcagcgcag ctctcgtcat cagtctgacc ggcaatatca tacagtactg
    23161 gcataacagc aacgtaacgc gtgaatactt tgccaccgac aatggccggc tggtccgcct
    23221 ggcccccacc agtcagccag cctggagtca gagcgacgtc atgacgtttg gcagcatgac
    23281 gctgagcgaa gcttttaatc tggattttgt ccactacagg aaacaaatca cgacactggc
    23341 cccccgcttt tccgaagccg gttttgccgg ctatgtgaat gctcttcagg cctccaacat
    23401 tctggacacc attaaaaagg agaaatttaa cctcacggcc accatcgggg ccggcacgct
    23461 ggtgaatcag ggccagatgg aaaacggcat ctggttctgg aaattccagt atccggtcag
    23521 gatgcgtctg gtcggtcaga gcagcacccg ccccgagcag gccttcacct ttgaaatcac
    23581 tgtccggcgc gttgacccac gaatcaaacc cggcggcatg gaaatcagcc agatggtttc
    23641 ccgtaacgcc gcatcaggaa cctgagactc atatcatgca aatacatcat cttcttggcg
    23701 cactgcttat tgtgtgcggc actgtcacgg ctgcagataa cccgagctgg cagaacgcac
    23761 gaaccccaca ggctggcgga aagacaggac aggctcccgg gaacgccccc gcaggcggcc
    23821 cccc
//
LOCUS       DAZUFB010000066        23542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZUFB010000066 DAZUFB010000000
VERSION     DAZUFB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.066"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(199..417)
                     /locus_tag="ACHY3M_003045"
     CDS             complement(199..417)
                     /locus_tag="ACHY3M_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317921.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCALGVNS"
     gene            complement(1749..1931)
                     /locus_tag="ACHY3M_003046"
     CDS             complement(1749..1931)
                     /locus_tag="ACHY3M_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317922.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1870..3960
                     /gene="iha"
                     /locus_tag="ACHY3M_003047"
     CDS             1870..3960
                     /gene="iha"
                     /locus_tag="ACHY3M_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFS4317923.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(4178..4337)
                     /locus_tag="ACHY3M_003048"
                     /pseudo
     CDS             complement(4178..4337)
                     /locus_tag="ACHY3M_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4540..4860)
                     /locus_tag="ACHY3M_003049"
                     /pseudo
     CDS             complement(4540..4860)
                     /locus_tag="ACHY3M_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(5285..5860)
                     /locus_tag="ACHY3M_003050"
     CDS             complement(5285..5860)
                     /locus_tag="ACHY3M_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4317924.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5929..6507)
                     /gene="terD"
                     /locus_tag="ACHY3M_003051"
     CDS             complement(5929..6507)
                     /gene="terD"
                     /locus_tag="ACHY3M_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFS4317925.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(6556..7596)
                     /gene="terC"
                     /locus_tag="ACHY3M_003052"
     CDS             complement(6556..7596)
                     /gene="terC"
                     /locus_tag="ACHY3M_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFS4317926.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7619..8074)
                     /locus_tag="ACHY3M_003053"
     CDS             complement(7619..8074)
                     /locus_tag="ACHY3M_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS4317927.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(8097..9254)
                     /locus_tag="ACHY3M_003054"
     CDS             complement(8097..9254)
                     /locus_tag="ACHY3M_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4317928.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(9254..9835)
                     /locus_tag="ACHY3M_003055"
     CDS             complement(9254..9835)
                     /locus_tag="ACHY3M_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4317929.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            10157..11215
                     /locus_tag="ACHY3M_003056"
     CDS             10157..11215
                     /locus_tag="ACHY3M_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS4317930.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            11225..12367
                     /locus_tag="ACHY3M_003057"
     CDS             11225..12367
                     /locus_tag="ACHY3M_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFS4317931.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            12360..13133
                     /locus_tag="ACHY3M_003058"
     CDS             12360..13133
                     /locus_tag="ACHY3M_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4317932.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            13135..14214
                     /locus_tag="ACHY3M_003059"
     CDS             13135..14214
                     /locus_tag="ACHY3M_003059"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFS4317933.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            14214..15170
                     /locus_tag="ACHY3M_003060"
     CDS             14214..15170
                     /locus_tag="ACHY3M_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFS4317934.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            15181..16389
                     /locus_tag="ACHY3M_003061"
     CDS             15181..16389
                     /locus_tag="ACHY3M_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFS4317935.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            16407..16874
                     /gene="terW"
                     /locus_tag="ACHY3M_003062"
     CDS             16407..16874
                     /gene="terW"
                     /locus_tag="ACHY3M_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFS4317936.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            17135..17464
                     /locus_tag="ACHY3M_003063"
     CDS             17135..17464
                     /locus_tag="ACHY3M_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4317937.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            17451..17792
                     /gene="tnpB"
                     /locus_tag="ACHY3M_003064"
     CDS             17451..17792
                     /gene="tnpB"
                     /locus_tag="ACHY3M_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4317938.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWMCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            17874..18704
                     /locus_tag="ACHY3M_003065"
                     /pseudo
     CDS             17874..18704
                     /locus_tag="ACHY3M_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18735..20348)
                     /locus_tag="ACHY3M_003066"
     CDS             complement(18735..20348)
                     /locus_tag="ACHY3M_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFS4317939.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(20379..20729)
                     /gene="tnpB"
                     /locus_tag="ACHY3M_003067"
     CDS             complement(20379..20729)
                     /gene="tnpB"
                     /locus_tag="ACHY3M_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4317940.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20726..21151)
                     /locus_tag="ACHY3M_003068"
     CDS             complement(20726..21151)
                     /locus_tag="ACHY3M_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4317941.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            21205..21956
                     /locus_tag="ACHY3M_003069"
                     /pseudo
     CDS             21205..21956
                     /locus_tag="ACHY3M_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         5943 a         5992 c         5876 g         5731 t
ORIGIN      
        1 ttatgcatga cctacctgaa tttgctccgg gtagaggtta taaataaaaa ttgaatcacg
       61 acaaacacaa tattcatagt atggcgatgc ctacgccagc aagaatagcg ccaataatat
      121 tgggaatata atagacacca gacgcacagg catctcactc cttaacaaac aacaatcaga
      181 atatttactt ttaccaagct aactgtttac acccaaagca cacacaatta accattcaat
      241 aacaaatggc aatatccata gccatacgac tttaccttgt aatgttcggt atttctttat
      301 aattattctg ggaaatctaa catttatttt taaaatcaaa ttatcttgtt gtttaaaaat
      361 aagttcacat actttatcat cttcttccgc catcaacatc tctgcatact taaacatttc
      421 agaacgttcc tttagcacag aagagtaatt atatgtccag ttcccaggca ataatgctta
      481 tggatattta attcataatt tagagaatat tttgcaataa tatttggcag tattcagaaa
      541 tacctgaaaa atcatactat acagccctag ggaatggata aagattctaa caaagcattt
      601 caacaatata tacttgttaa aaatccatat cgaaatgccg ttgcagcatt aatatatgcc
      661 tcattcataa aatgtaaaag agcaagctcg taccagtagg ggggaaattc attgacatgt
      721 cctgtcaata cgtacagagc cctgccatgc ttgccagcac gtaacaattc cgcccccagt
      781 aaccagcgtg ttccctgatt attctcaggg ttatatgtta gtattttatc aatgagtata
      841 acggcatcct gatgacgctg taaatgaaca ttcgccagaa ttgcagcata aagagcgcaa
      901 atgcatatgc cagatgagcg tgaatatcaa tgatgtcagg agcatgtcag gcaagacgcc
      961 tgagtttgtt gacgtaattt gtttcgttta tttcatcact atcgtatgcg tcaagagttt
     1021 tttcaaactc atcccgaaat gggccaagcc tggtttcagg aaataagaaa tacccttggt
     1081 tattgttcac ttaaaagtat aatttgtaat tcaaatcctg agcctgtaaa gcggggagta
     1141 acatattcta ttctgaagag aataaaagtc gtgatgcgat gtatcaagcc cggattgtaa
     1201 tcccagatta acatagatca caaaacaact tattcttcac taacgtcaag ataaatatcg
     1261 ttgtatgcct tatcacgact acgaatacca gcaagataca actgacatac ggaaagatac
     1321 cacgtttttt actcccgaaa ataacgctaa aaagctactt ccccatcgtt tgtccttagt
     1381 attgccagcg ccaacaatgt gggctgacat gataaagctg tctaggaaat tgttcgcctc
     1441 ctcagcggac aatccaaatg gtgattgtct ctgttaaacg tttattttga aggtcgactg
     1501 aataaggtga tgacgctgta gaatttttca cgtgccacag aattttgaac gctttctctt
     1561 acaatatttc aatgtttcta tcagtattcg ccggaagaag tcatcgacca aatcatccca
     1621 gtcgtctcgc atcactgacc attcatggtt gacatgtggt ggaaatccgc ttctacagta
     1681 accatttttt attcgcaaaa ccgaacacgc catacggata gctgttaact ggcatgccga
     1741 ggcagtcgtt atttatattt ggttttgtca ataatcttta ttttttgtaa aagacaaata
     1801 taaattattc tcattattgt ttgtatttgt gtattgtctt gccggttaac atgatcggag
     1861 attagtaata tgcgaataac cactctggct tccgtagtca ttccctgtct cggattttca
     1921 gccagcagca tagctgctgc agaggatgtg atgattgtct cggcatccgg ctatgagaaa
     1981 aagctgacta acgcagccgc cagtgtttct gtgattagcc aggaggaatt gcagtccagc
     2041 cagtaccacg atctggcgga ggctctgaga tcagtagagg gtgtggatgt tgaaagtggt
     2101 acgggtaaaa ccggagggct ggaaatcagc atccgaggaa tgccagccag ttacacgctg
     2161 atactgattg atggtgttcg tcagggcgga agcagtgacg tgactcccaa cggtttttct
     2221 gccatgaata ccgggttcat gccccctctg gccgccattg agcgtattga ggttatcagg
     2281 gggccgatgt ccacactgta tggctctgat gcgatgggcg gtgtggtgaa tatcattacc
     2341 agaaagaatg cagacaaatg gctctcttcc gtcaatgcag ggctgaatct gcaggaaagc
     2401 aacaaatggg gtaacagcag ccagtttaat ttctggagca gtggtcccct tgtggatgat
     2461 tctgtcagcc tgcaggtacg cggtagcaca caacagcgtc agggttcatc ggtcacatca
     2521 ctgagcgata cagcagccac gcgtattcct tatcccacgg agtcacagaa ttataatctt
     2581 ggtgcacgtc ttgactggaa ggcgtcggag caggatgtgc tctggtttga tatggatacc
     2641 acccggcagc gttatgataa ccgggatggg caactgggga gtctgacggg gggatatgac
     2701 cggaccctgc gctatgagcg aaacaaaatt tcagctggct atgatcatac tttcaccttc
     2761 ggaacatgga aatcgtatct gaactggaac gagacagaaa ataaaggtcg tgagcttgta
     2821 cgcagtgtac tgaagcgcga caaatggggg cttgccggtc agccgcggga gcttaaggaa
     2881 tcgaacctta tcctgaattc attactgctt acccctctgg gagaatctca tctggttacg
     2941 gtggggggcg agtttcagag ctcgtccatg aaagacggag ttgtccttgc cagcacaggt
     3001 gaaactttcc ggcagaaaag ctggtcggta tttgctgagg atgagtggca tctcacggat
     3061 gcacttgcgc tgactgcggg cagccgctat gaacatcatg agcaattcgg gggacacttc
     3121 agtccgcgtg catatctggt ctgggatgtg gcagatgcct ggacgctgaa aggcggtgtg
     3181 accacgggat ataaggcacc cagaatgggg cagctacata aagggattag tggtgtgtcc
     3241 gggcagggaa aaacaaatct acttggtaac cccgacctga agccggaaga gagcgtcagt
     3301 tatgaggctg gggtgtatta cgataacccc gccggtctga atgccaatgt cacaggtttt
     3361 atgactgact tctccaacaa gattgtctct tattccataa atgataacac caatagctat
     3421 gtaaacagcg gaaaggcccg gttgcacggt gtggaatttg ccggcacatt gccgctgtgg
     3481 tcagaggatg tcacgctgtc actgaattac acctggaccc gaagtgaaca acgtgatggt
     3541 gataacaaag gtgcgccgct gagttatacc cctgaacaca tggtgaatgc gaaactgaac
     3601 tggcagatca ccgaagaggt ggcatcatgg ctgggtgccc gttatcgcgg gaaaacacca
     3661 cgtttcaccc agaattattc gtcactgagc gctgtacaga agaaagtgta tgatgagaaa
     3721 ggagaatacc tgaaagcctg gacggtggtg gatgcaggtc tgtcgtggaa gatgacggat
     3781 gccctgacgc tgaatgctgc ggtgaataac ctgctcaaca aggattacag tgacgtgagc
     3841 ctgtacagtg ccggtaagag tacgctgtat gccggtgatt acttccagac gggatcatca
     3901 acaacaggat atgtgatacc tgagcgaaat tactggatgt cgctgaacta tcagttctga
     3961 taataacaaa acgctatcac tgacggtaga atacgttgcc actgcaactc ctggcggaac
     4021 agtggcaacg tattaggtta agtgcatttc cgatccgcta atgagatttc gttaccaaca
     4081 actaatatcg tcacaggaaa tgcacggatt atttttaact tatcatttac atacttgtcc
     4141 agagtgtaag cgcaccgtga aggacgtggg gtaaaaatta gtttacagag agagtgacgt
     4201 tccagggcaa caactctttc atgcggttgg caggccaggt gttgattaca ctgatcacgt
     4261 ggcgtagcca cgtttccggc tcgattccgt taagttttgc agctaccgat caggctgtac
     4321 atcactgccg cactatcgct cgtcatctca aagtccggtc tcgtcagcag gaaggtatca
     4381 ttctctcccg ccatttttcc aggggcccgg tcagataagt ccctttgtct atcgctgact
     4441 cctgactcat aacccggtta gcagaatgca gggtcaccac ctcgccacga ccaaatccaa
     4501 ataagtcaat tgcaccttct caatcgccat tttgtcagta agcgtacagc ctgaactgat
     4561 ggtatcttca ccatcaatga caacggtgat cgcaatttta ctgacgttcg ccggaacacg
     4621 atccagtgct atctcaatgc tggcctgctg cgaaccggta acgagcctga ctgccccctc
     4681 aggagaagac aaattattat aaaagataaa gtcagaatcg ccactgacct ttccctgagc
     4741 attaagcatg aacaggcagg tatcgggttc gcctttaaag ccggatttgg cagggtactg
     4801 aagattcagc ctgatcgtag attgctgaag gggtatgttt tgtccggatt gtaaattcat
     4861 attaactctc ctgatttttg attattatta atgcgcagcg tttatatatg ttccctaggc
     4921 ttagttctgg acgctggata ttcggtgagg cgtaaatatg gtatgacacc atttttcata
     4981 acgctgaagt ttctatacct gttgaatttg aattttcatt gaccgggtat cttattttcc
     5041 agggcccgtt ccttcataag tcgcaaaagt acacatatat ccgaagggca tgctgttgat
     5101 atcagacacg gaatactggc tttaaccagc aaccacagat aaacccgggg cctgcataaa
     5161 aggttcatgc cagaattaac atagctctgc tttctgcccc cccccctttc ccatattcac
     5221 ccgttgatag cggatcgata cccaaaaaaa accccggcat tgccggggtc agactcagcg
     5281 tcttttagac attgacgccg tgctgggaag caagcgctga cagaccaccg gcaaaaccct
     5341 ggccaacagc tttaaacttc cactcagtac catggcgata cagttcaccg aagaccattg
     5401 cggtttcggt tgaggcgtct tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt
     5461 tgttgtaaac gcgcatgaag ctgttgctca ccatgccgaa gttttgttta cgcgcttctg
     5521 catcatagat ggtaacggca aataccagtt ttttgatgtc tgctgagact ttggtcagat
     5581 cgattttgac ctgctcatcg tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct
     5641 ctactgcgcc atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagta
     5701 ctttaccgtc ttcacctact gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg
     5761 ttacacgggc atcccagccc aggccaacca tagcgacatt catggttggt gcttctttgg
     5821 tcagagatac gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt
     5881 tgaatgaggc tgaattaaca gccccagata tgaaaagtta cttttcgatc aggacgcgtt
     5941 aatgccgtac tgagcacata cagatgccag accaccagca taaccctggc ctactgcgcg
     6001 gaatttccac tcaccattgt ggcgatacag ctcgccgaac agcatggcag tctcagtgga
     6061 cgcatcttcg gtcagatcgt agcgagcgac ttcagtctgg ttatcgtcat taaccagacg
     6121 aataaacgca ccggatacct gaccaaagct ctggcgacga gcctgagcat cgtggatggt
     6181 cacaacgaag atgatcttgt caacttcaga cgggacggcg tccagtttaa ttttcagcga
     6241 ttcatcatca ccatcgccct caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc
     6301 ggatgacgtc aggttgttat agaagatgaa atctgaatcg ccgcgcactt tgccgtttga
     6361 ggccagcagg aatgctgaag catccaggtc aaagtcctga ccgtctgttg aacgcgcatc
     6421 ccagccaagg cccaccagga catttttcat tgacggagct gctttactca gggagacgtt
     6481 cccgcctttg gaaagagaaa cactcataaa ataacctctt cgattagtaa ttgttcaggt
     6541 taacacttaa ggggattaac tccccttttc ctcagattca ggtttgcccg ggaacatgac
     6601 gcttgcgaga atgcccagcg ccagtacacc cagtacaaca tacaggctgg ttgttgccgc
     6661 gatgctgtaa ccatgatgcc agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat
     6721 gaagaacagc agcacgataa cggccttctc cagatgaacc aggtactgtt tcagtgcctc
     6781 aaggacaaaa tacagagtac gcagacccag gatagcaaac atcatggcac tatagacgat
     6841 aagcggttca cgactgacgg caatgattgc cggtaccgag tcaaacgcga acattacgtc
     6901 cgagagttca accacagcca cacacaggaa cagcggggtc gcatacagcg ccgctttttt
     6961 accacggcca atggtgacat cgctgttttc tggtttcgcc agttcagcat ccacttcctt
     7021 ctggttaagt aggaaggcat gccctctgag cttcggccag atagggaaga agcgtttaac
     7081 catacggtaa gccagatgct gggagtaatc ctcaatttca tcatcgtcat caccgctttt
     7141 aagcatcatg accgccgtcc aggcaacgat aatagcgaag acaacttcaa cgtacggccc
     7201 cagactcagc aggctcgtac cgatggcgac aaagatgccc ctgaagacga tggcaccaat
     7261 gatcccccag tagagaacac ggtggcgata acgatccgga acggcgaacc aggagaagat
     7321 tgccatcatg acgaacaggt tatcgaccga cagcactttc tccagcgcat aacccgtgac
     7381 aaacagactg gcaacctcag caccgtggtg gatatagagg aaaccggcaa atgccatcgc
     7441 aaccacaacc cagaatacgg accagagcgc agcactcttc agcgagattg gcttgtcatc
     7501 acggtgcata aacaggtcaa taaagatggc accgactgaa agcgcaataa aaacaatgac
     7561 cgtttcagtc gggaagccga tgtgtgtgga aaccatattt tacccttagg gatgtggatc
     7621 agaggccaaa ttcagccggg tcgaagccca acgcgatagc gatctcgcgg gaggccagtt
     7681 tctcgtcctg atcgaagtta ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca
     7741 gctgagcggc ctcaggctga tctttcagcg ccaggatgta tttcatggtt tcgcccttgc
     7801 caatttcaac atcgaaatcg aagcttgaaa ccagtttatt gaagaactca atcacctcat
     7861 tggtatcgaa gaccttcagc tcttctgaag agcgcagaaa gcccatcatt ttctgctttt
     7921 cttccgaact tacgccgtca ctcgatacgg caatacgggc acatacagca acggtgccct
     7981 gcatgaattt tttgtttttg aaacggccaa cctggcgggt cagttcgtca cggcctgagt
     8041 taatggcacc tttaactttg tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat
     8101 ttagaaccgg cgctccagcg aaatccccag ccaaatgccc ggtccatttc gtcctggcct
     8161 ttgaagtact ggttaattcg ctcgacttta attgcgccgt tttcgtttac gagtctggca
     8221 atggcgcaca atgtgcgacg gttctcacct tcggtcaggc gggtctcaat gggcggttga
     8281 tccggcacat gaatggtgac tacaccatca gtcttgtccc agctgggaac accttcataa
     8341 ataaaggcgt aaatcagcac ttcgcggata tgcttccatt cacgtccatt gatatgcagc
     8401 cactcgccgt ctgatacatc acccgtccgg tcatcgccct tgagctgaac atacggttca
     8461 tcgcgataat caccgaatgc attaccgagc gcctgaatga ccgatttata cccgtcctga
     8521 agctcaacaa aggcccccag atccaggtcg attcctttgg agccgaacat acctgcaaag
     8581 cctgatttgc tactgccccg gtgccagttc aggttgatcc ggatttcacc gaagttatcc
     8641 cgtttggtca ggctgattgc aggcttctct tttgtcagcg ataccttgct caggctgatt
     8701 tttgtctcaa ctggcggagg ggttacaacc ggaggagttg gggctgcggg agccggttcg
     8761 tcggcaatat ttacgccgaa atgctctgcc agcggtttaa gtccaccgtt gaacccctgc
     8821 gcaacgaagc ggaatttcca gtcattatta cgacggtaaa attcccccag aattaaggct
     8881 gcttcctgac ggccgctcag ttcgacactg ccgctaacca ggccggtagc cccttgctcc
     8941 acgtcgatgg aaagattacg aagaccggac accgtctgcc caccgtcaca ggtcacggtg
     9001 aacgcaattt tctggacatc aggtttcagc cggttgagcg cgacagtaaa ggttgagtac
     9061 tggccttcac tgaccaggct gacggtacca tcatcgttac gtggctgacc ataaaacacc
     9121 atgtccgcat caccctgcac cttcccgtcg gcatacaggc gaaacgccga agcatccacc
     9181 tggccgcccg aggtgatccg gacccgcaac tcctgggctg gaacgggggc attgcccccc
     9241 ggcgtcaggt tcattagcga ataaccgctg aaacgatatc ggagtgcata tcgtcaattg
     9301 tgcggccgcg acaggcatgg ccaagggcgg taaagtccca gttgccgtta ttacggcgca
     9361 gcgaagaaat aacgatcccg gtatgtgagc cctgctcagt cagcttgtag cgagccagct
     9421 ctttgccggt ttgatcgaca acgcggcaga aagcgttttc gacgtcattg aacgactgac
     9481 cacggaagct gtttaccgta aacgccaggt attcaacgtt tgcaggcagg cgggagaggt
     9541 tgaccttaat cacttcatcg tcaccgtcac cctcaccggt caggttatcg ccactgtgca
     9601 caacagcgcc gcaggtggat ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg
     9661 tgctgtccat taatacacag ccagcatcga gatcgattga gtcgttgcct ccaaacaatc
     9721 ctccaagaag accttttttc ttaaccggat cccaaccgag accgaaatga agctggctta
     9781 atgcagatga ttctttgctg agtgataccg tctggttctt aactaatgaa accatgtttt
     9841 attccttaaa atggtaatga ccgagacaat caaaggtata tttagtaact gacgaagaaa
     9901 atcatatcat gatgaagttc tctgacaagg gaaatttttg tggttttctg taaaaacaca
     9961 aaaagaaatc cttttcaggg tgttggtttt tctatttttg atttaaatat caatgtgtta
    10021 taaggaatcc aaaaaacact gacgcactcc ataaccacag gagttattaa tcatattatg
    10081 attgacatgt agcttagaca ctcatagact cacttctcaa tcataaattt gcatggcgtt
    10141 ccggataatt aaaaaaatga acaagaaaat ttggtttatg gaaggtttat cctcccagcg
    10201 agatattatt cagggggtaa aatcatttgc acaaaaaaat aattttgcca ttaccgtttt
    10261 tgcctcccac cgtaacgaaa gaaatgaaat cctttccgtt gccgattatt ctttgactga
    10321 acctgaagat cctcaaaaac gtcttcagtt tatccaggaa accattcaga cctacggcat
    10381 ccaccatatt catactggcc gtaacagcca gtggtttgaa gaacaccgtt cagccattga
    10441 accgaccggt gccaccctta ctaccggtgc aacgggcgtc gactggttaa ctctggctga
    10501 cgaaaaagtt acttttgctc agtttatgga gcaaaagggt ctcccggtcg taccatcctg
    10561 gcgggtgaat acgctggcag aattaaagac acacctcgcg gccccgccgt tcactgacag
    10621 cccggtatgc gtgaagccgg tgacgggtat ctatggcatg ggattctggc gctttgatga
    10681 cagtgcttcg cctatggccg tctttaatca tcccgaacat cgtctggtca gtccgcaaca
    10741 gtatattgca gcagcatcag ctgctgagtc gtttaaaccc cttgttttga tgccgtacct
    10801 gccaggcccg gaattttccg tcgatatcct cgcggataag ggcgaaatac tcgcagccgt
    10861 gggacgccgt aaggaagggg ctatccagta tctggtaaac gaaggaagcg cctgggaact
    10921 ggcgtgtgac tgcgcccgtg ttatgaaggc cgacgggctg gtgaatgttc agacgcgaaa
    10981 cgatgtgaat ggcaacccgg tgctgcttga aaccaacatg cgtccgtcag ggggggtggg
    11041 ttataccctt cacagcggtg tgaaccttcc tgggttattt gctgccttta agctcggtct
    11101 gatgtctgaa gatatggtac gccagagcgc taaaaacacc ttttctccgg ttgcggtgag
    11161 atccattacg gatgtaattg catacccgga atcactctct aaccttctga attaaggcgt
    11221 atttatgtct gatatcctcg aagatatgat ttatcgccgt accctctcat gcggtacgat
    11281 tcaggtaacc cgtgaccagg gtgaagtttc gctcgatgac ctgtttgaca tcgctgaaag
    11341 acgtaacccg aaacgcgcct ttctgtttgt cagcaaagta ctcggcaggc acattcccgt
    11401 gccaccctca gtcatgcggc aggcctacag acagcttgcc agccagttcc cctcgacact
    11461 gacaggaccc gtactgttta tcggtatggc tgaaaccgcc gttgggcttg gggccggtgt
    11521 atttgatgaa gtgcgccacc agcatcgtga atctgtctat ctgacctcta cccgtcaccc
    11581 ggttgatggc acgttgcttt gcgagttcaa ggaagaacac agccacgcca ccgatcatct
    11641 gatctatctg ccagatgatg aagagaaaag acgtcgtgtt accaacgcac gaacgctggt
    11701 tttgattgat gacgaggcaa ccaccggtaa tacctttatt aacctgcttt ctgccctgcg
    11761 taataccggc aagcttcaac atattgaaca ggttatcgcc gtaacgctga ccgactggag
    11821 tggcaatgcc ctgtcggagc gcaccccctt gcccgtcact tcggtttcgc ttgtaagcgg
    11881 tcagtgggga tggaccccat tacctgatgc acctgtcccg gacatgccga aagtcaacgt
    11941 aacttcacga ggagaatggg acatccaggg gaaacagtca tggggccgac tggggatgct
    12001 cgcacctgcg gccgatctcg gccatgaggt ctccgtccac aagggggaac gtattctggt
    12061 tctcgggacc ggggaattcg tctgggagcc gttcctgctt gctgaacggc tcgaagctgc
    12121 cggagcacag gcattttatg gatcgaccac ccgctcccct gtcgccgttg gttatgccat
    12181 tgagtccgcc atttccttta cggataacta cgggctgggc atccccaatt ttgtctataa
    12241 cgtcgcccac cagcagtttg accgcattct tgtgtgtact gagacacccg cagaaagtat
    12301 tgacacgcag cttcttaaag cgctggctga ggttgcgccc gtcgtggaga ttgttaccta
    12361 tgaataaacc cgttgttctg agcgaccttg acgacacgct ctttcagacc cgtcgtaaaa
    12421 tggtggacga gctggccctg gagccattcc gtaccggtgc cgttgatcgc accctgaatc
    12481 cacgaagctt tatgacggaa gaacagtcca tgttggtgga ctggctcctg gagcaggctg
    12541 aactcatacc cgttaccgca cggggaactg aagaaatcag ccgcgtacgg atccctttcc
    12601 actcctgggc aatcacaacg cacggggccg tcattctcac gcctgaaggc aaacccgacg
    12661 aggagtggaa ggcccatatg ctcggccagc tggctcccta ccaggaaaag ctgacatcga
    12721 tgcagcgtct gatcactgaa atgatggacg caaaaggaat caatgcctgg gcaaggctga
    12781 acttcgaata cggtgaaacg gcggtttata tggtgatgaa acaccgcgac agcacccgcc
    12841 ttgacgagct caatgccatt gcagatgaga tagaaacggt gtttccgacc gagggcttct
    12901 acatccaccg caacagtaat aacgtggcct ggcttcccac cccggttgag aaagggctgg
    12961 cagtcagctg gctgcttgaa aaacttcggg ctgaacgagg agttttcccc gtaattggtt
    13021 tgggcgacag cctgagcgat catcgtttta tgaaactgtg cagctggttt ggcatcccgc
    13081 gtcagagtca gtttgcagat gccatttcac agcgaatttt tggagaaaat taaaatgcag
    13141 ccacatgaaa catttaccgg ctcataccag cccggtgacg tggaatttct gctaaagccg
    13201 gttgtcattg agatgacgcc ggttgagaaa aaagaagaac tgattcagtc cgggaagaaa
    13261 cattattcgg acatgctcag ccaggagcca gcgcctacac aatggcatct tgatttgttt
    13321 caccgggcgc tggatcgggg cgcagagagg cttgcaaaag aagtcacaca gctcgctatt
    13381 gctctggccg aacgcttcgg tgatgagccc attgtactgg ccagtcttgt cagggctggc
    13441 gttccgctcg gcgttatgct gcaccaggcc ctgcgtgaca tggggaaaac ctcatggcat
    13501 tacggtatca gcattatccg ggatcgtgga attgacggtg cagccctcga cgtcattgaa
    13561 gagcggcatg gtaccagcgg tattgtcttt gttgacggat ggacaggtaa aggcgcaatt
    13621 accggagagc ttgtacgcgc actgaaagat cgcccgggct atcctgagca gccgcgactg
    13681 gttgtcctgg ccgatccctg tggctgctcc tggcttgcag ccagtgatga tgactggctc
    13741 attccttttg gcatcatggg ggcgccggta tcaggcctga tctcccggtc agtatggtcc
    13801 tctgaaggat tacacgggtg catggtttgc gagcatctca gtgaattcga atgcagccgg
    13861 atgcttgccg ataccgtcgc tcatttccgt aagaagttaa caccgtcatc cctcgctccc
    13921 ctgagctgga atacggaatc agcccgggtc ttatggcaga caagtcgcga cgttatcgcg
    13981 ttcctggccg atgaatttaa agtggacagc gtcaatcgta ttaaacccgg tattgctgaa
    14041 gcaacccggg ctgtattacg tcgggtaccg gaccatgtat ttgtgcgttc tattgacgac
    14101 ccggacgttg ccttgcttgt agggcttgct cgtgaaaagg gaatagttgt tacagaaatg
    14161 gggggaaccc tcggccagta tcgggctgta accattatca agaaggtact ctgatgaaaa
    14221 atcgtctttc tccctggaat ctgggagcca cgctatacat gcctgcaaca cgggaagata
    14281 ttgctgatgc cgtcctgcac gggaaaatcc cgggcttgcg ctctctggtg atttgcctgg
    14341 aggatgctgt cagtgaagca gacatccccg tcgccctcaa aaatctggag cacctgctgc
    14401 acgagctcag taacagcatg agtagcctgg gtaaaaatga ctggcctctg gtgttcattc
    14461 gcccccggca tgccgaaatg ggcagatggt taaaagcgca ttatgatctt tccgctgtcg
    14521 acgggtttgt gctgcctaaa tttaccctca gttcgttagc tgaatggtgg gatatcatgg
    14581 tcgggactca cctgtgcatg atgccaacgc tggaaacaga agatgtcttt gacgtggttc
    14641 agatgcgcga gctggctacc cgtctggagg aacatccctg ccacgaccgc attattgcgc
    14701 ttcgaatcgg cggcaacgat cttatgaacg ttgtgtcgct tcgccgtccc cgggacctga
    14761 cgctgtatga cagcccgatg ggctacgtca tcaaaatgct cgtttcggtc ttcggcccgc
    14821 gtgattttgc cctgaccgcg cctgtatgtg agcatattga cgatcatgcc gttatggcca
    14881 gagagctggc tcttgatatg gcacacgggc ttgttgggaa aacggccatt cacccgggtc
    14941 agatagaggt cattcaaaac gcgctgatgg tcactcaggg tgagcattct gacgctctgc
    15001 ggatcctgaa ctctacccag gccgtgttta agtcgcaggg agcaatgtgt gaacccgcca
    15061 cacatcgccg ctgggcggct ggcattctgg acagggctcg tttttatggg ttacagaacg
    15121 agcaaagcgc tgatggaatc agattactta ccgtgaccca gcatcattaa ggaaatcata
    15181 atggggttca ggttcagaaa gtcgatcaac attattcctg gcgttcgcct caacctgagt
    15241 aacggtgcac cgagcctgag tgtcgggccg agaggtgctt ccgtttcttt tggtagccgg
    15301 gggacctatg ccaatctggg cttgcccggt accgggctga gttaccgtac ccggcttgac
    15361 cgggccgcgc gttccagagg tgaaaaccgg acggcaaccg acccggggct cagacaggcg
    15421 cttgagcaga aagccgctga actcatgtca gcggtaaccg caatccgtaa tattcacgag
    15481 ctgacgccgg atccaaaaac aggcatcagc tgggcagagc tggaagcagt atacctgcat
    15541 aacagaacgt cgccttttca ggttccggca ccggtgcgtc cagaaaagcc agactacctt
    15601 gtattgccgg aaaagcctgc cgagagcgaa ggcattagtt ttttgggtaa atggtttgaa
    15661 tcggaatcag ctaaagctga gcgccacgcc gaaaatcttc gccggtggca gcaggagctg
    15721 attgatgtgg agcgtgagaa tacccttcga cagcaccggt accagcaaca acggacggcc
    15781 tgggccgaac agtatgcaaa ctggaagttt gaagctgaag aacatgaaaa acggctcgcc
    15841 acggctcagg cagatgcccg gcagcagttc cggacagacg ccgcgttttt cgaatcatac
    15901 ctggcgggtg tgctggcaga aactgaatgg ccgcgtgaaa cgcttgttgc atttgaagta
    15961 aagccggagc tatcagcagt cctgctggac gttgatttag ctgagattga agatttccct
    16021 gataagattt acggcgttaa tgcccgggga acggagctga cggaaaaagc catgacgcaa
    16081 aaagccgtac gcgaaaacta tgcccaccac gtccatggct gcttgttccg cctggtcggt
    16141 atcgttttac atacgctacc tttcgacaac gtgattgtgt caggctttac gcaacgggtc
    16201 agtaagcgga ccggctatct ggaggatgag tatatcctgt cctgcaaatg cactcgcagc
    16261 cagatgtcgt cagtaaattt tgcaggcata aaacacattg atccggttga agcgttaggc
    16321 gatcaaccgg ttattcgaaa gatgagcagt accttcattt ttcagcctat tgaaccacta
    16381 accctttaag caggctaaat cttacgatgc aattaaatac cagacaggcc cggattttta
    16441 agcttgccaa cttactgggc accggcaagc ccgtttccgc tgccgacatt ataaccagtc
    16501 tggaatgctc tgagccgaca ttaacccggg ccctcaaaga gctacgcgag tcatattctg
    16561 cggagatcaa atacagcaag gcaggtcatt cctatcatct cgttaatccg ggccagttgg
    16621 ataaaaagac cctccgccgg atgaatgaag cgctcgcaca gaatgctgaa ctgaaaaccg
    16681 gtgagtccac agggaaggtc gtactcgata aggataaaaa aacagccgtg tcattatcgc
    16741 tacgcatgcg gattctaaga aaaattgacc gtctggctgc gctgagtgac tcgacccgaa
    16801 gtgaagcggt agagaagcta gctctgcact ccgttgatga actgataaaa gaatacagcg
    16861 ctaaaaagtc ctgacgctaa atctcagacc ggggtgaaaa ctccggtttt gtcatatttc
    16921 acctctgtat tggcgtgatg caaaatggca tctgaatcga tcatggtttg gcatccattt
    16981 ccgatcactg tttggcatca caatcgatca cgatttggca tgcttccgct cattgattgg
    17041 catcctggtg tcgttacggg gaagtcatct tcacagggat cttcagtcag ttagcattac
    17101 cgacatccat tcaccaactt accggagata atcaatgtca aaaccccgat ggacgcttga
    17161 tcagaaaaaa catcacgttg ccgcatggcg cgccagcggc cttacccgtg aacagtactg
    17221 tgaactttac gacattccgt ttaaatccct gcgccagtgg ccgcaggatg tcgcaaaagc
    17281 cgaaaaacga gccagagcac cagaaattat tcctgtttca gtatctggct cttccggaat
    17341 gactgatggc agaccattgt cggatgagcc tgtcactctc ttcctgcccg gtggtatccg
    17401 gatgtgctgc cagccttctc agttaaccga tgttttccgg gctcttcgcc atgctgacgc
    17461 ctgataacgt attcctggtc gtaaaacccg tcgatatgcg ccggggtatc gacacactga
    17521 cacagtacgt ccagaacgaa cttaacgctg catggcatga cggtgctgct tttgtcttca
    17581 ccaataaagt ccgctcccgc ataaaagttc tccggtggga caaacatggc gtatggatgt
    17641 gtacccgacg actgcaccgg ggaagcttcc gctggccccg gaaaggtgat gccacatggc
    17701 atctcacaca ggatgagttt cactggctgg tctttggggt tgactggcaa caggtgaaag
    17761 ggcatgacct ggcaaaatgg gtataccagt aagaacccga agatgctcag agcaacgcaa
    17821 taactcaatg aaatataatg aatatccatg cgtgatgtca tataatccac cgcatggata
    17881 tctcgcttct cagtaccacc agtgaccctg aacagcttcg cgctctggca attgccatgg
    17941 tgcaaaaagt catggctgag aatgcagaac ttcagaaccg tatccgcatc ctggaagagc
    18001 agatgaagct cgcacgccag cagcgtttcg gtaaaaaatg cgaatcgctg gccgggatgc
    18061 agcgttccct gttcgaagag gatgttgacg cggatatcgc agaaatctcg gcgcatctgg
    18121 acaaactgct tcctcagacc ggggacgaag aaaaaacaac aacacgtcct gttcgtaagc
    18181 ccttgccttc acctcttccc cgtgctgaaa aggttatccc gccagctgag gaacgttgtc
    18241 ctgactgtga tgcccccctg cacttcattc gcgatgaagt cagcgagaag ctggaatata
    18301 ttccggcaca ggtcgtggtc aaccgctata tccgcccgca gtacagctgt ccctgctgtg
    18361 agaaagtgtt cagcggaaaa atgccggcac atatccttcc gaaaagtgct gttgagccat
    18421 cggtcatcgc acaggttgtc atcagcaaat ataccgacca tctgcccctg tatcgccagc
    18481 agcacatttt ctcccgaatg ggggtggagc tccccgtcag tacaatggcg gatatggtcg
    18541 gtgttgctgg cgctgcactg gccccgctgg caaaattgtt acgccacgag cttctgacac
    18601 gggatgttat tcatgcagat gaaacgtccc agaggctcct tgatacccgg aaaggcggta
    18661 aatcctgctc cggctggctg tgggcttacg tcagcggaga aagagtaagc gtcaacggag
    18721 caccgtattg acgcttattt attggtgagt actacgttcc atggcaggag ttcatcaact
    18781 cggttggaag gccattctgg cagtacgctc aggatatggc gcagatacgc ttccggatcg
    18841 ataccgttca gacggcaggt gccgatcagc ccgtacaaca gtgctccacg ctcgccgccg
    18901 tgatcgctgc caaagaacat aaagtttttc tttccgagac agactgcacg aagcgctctt
    18961 tccgcagcat tattatccgc ctccgccaga ccgtcatcac tgtaataaca gagggcatcc
    19021 cactgattca gtacatagct gcacgcttcg cccagtctgg attttttcga cagcgtacca
    19081 ttcttctcca ccatccattc atgcagtgac gtcagtaaca ctttgcttcg ctgctgcctg
    19141 acggcaagac gctctgactc cggtaatccc cgtatttcat cctcgatggc gtacagttca
    19201 ctgattcgct tcagggcttc ttctgccgtc gcacttttgc tgctgatgta tacatcgtgg
    19261 atttttcgcc gggcatgggc ccagcacgca acttctgtca gtgcaccacc ttcacgttct
    19321 gcactgaaca gccgatcgta accgctgaac gcatccgcct gcaggatacc ccggaaggga
    19381 cgaaggtgtt gtaccggatg ttttccctgc ctgtctggtg agtaggcgaa ccagaccgcc
    19441 ggtggctctg atgagcccgc attccggtca tcccggacat acgtccagat gcgtcctgtt
    19501 tttgcctttt ttctgcccgg tgccagcact tttactggtg tgtcgtcagt gtgaaccttg
    19561 cgggtgttca tcacgtaacg gtacagggta tcattcagca gagtcattaa ctgacaacat
    19621 gcgtcaaccc agttggagag taatgcccgg ctcagttcga tgccctggcg ggcaaagatt
    19681 tcactctgac gatacagtgg caggtgttcg cagtattttc ccgttaacac gcgggcaagt
    19741 aatccggagc ccgcgatgcc gcgctctatc gggcgggacg gcgctggcgc ttcaactata
    19801 cagtcacatt ttgtacaggc tttttttacc cgaacagtgc ggatcacttt cagggcgcta
    19861 ctcaccagtt ccagctgctc agcactgact tcccccagat aatccagctc actgccacac
    19921 tccgggcaac aactttcttc aggctccaga cggtgtattt cacggggaag atgtgccggt
    19981 aacggacgac gatgacgtga ctgtcgcaac tggcggggaa cctgcgggtc atcctcacgc
    20041 ccactgtaac gatcgctttc ctgttcgcgt tgtttcagtt gggcctcagc ctgttcaacc
    20101 tcacgctgca gtttttcaga acgggtaccg aacaacatcc ggcgcagttt ttctatctgg
    20161 gccctcagat gttctatttc ccgctcctcc tcttcgatct tttcctcggc acgtgccagt
    20221 gcagagcgca ggaaggcctc cgtctcttca accagactca gttgctgatc tttctgacgg
    20281 agggcttcag cctgctcaga gagcagcctt tccagctcag tgatacgaat gaggtatttc
    20341 cgactcatga ccgtttttat aatccggcca tgacattttt acaacattgt cagtgcatta
    20401 aggcgggatg ttttgggctg acgccagtcc agtttatcga ggagcattgc cagctgcgag
    20461 cgggtaatgg ataccttacc gtcacgcacc gcaggccaga taaactggcc ttcctccaga
    20521 cgtttggtga acaggcacag accatcagca tcagcccaca gaattttaat cgtgtcaccc
    20581 cgtcggccgc gaaagataaa caggtgaccg gagaaggggt tctcatccag cacatgttgt
    20641 acctgttctc ccagaccgtt gaaggattta cgcatatcag taacgccggc aaccagccag
    20701 attcgagtgc ctgatgggag cgagatcatc gtcccctccc ggtcagttca cggatcaaca
    20761 ccgtgagcag ctctggtgaa ggattttcca gcgtcatgtt accgtggcgg aactcaactt
    20821 tacaggaact ggcactgact gtgctttgtg aaggagtgga taaaagcgga gtaagagccg
    20881 ccataggctc tttctgctca tcaggcgtta tctcaacagg taataattca acgccagcgc
    20941 cagaagaggt tgttaccgga agacgccgcg atatacgcct ttcgttctgc cagagcctga
    21001 gccatttgaa caggaggtta tcattgatat cgtgttcccg ggcaatacgg gcaacagagg
    21061 ctcctggttg tgaagccagt ttaaccattt gaagtttaaa ctcatttgaa aatgatctgc
    21121 ggggttctga ggataatatt ttctgttcca taacaggtgt ccactagttg aaaaggtggg
    21181 cacctacgtt accaatactg gcttaatggc tacatacggc ggtcagttta cgcttacgag
    21241 aaagaagcgg tccgccggtc gtctgttttg actcgcagac cggacgggcc ctcagatatc
    21301 ctgaggcctg gctgcagggc tggtgtggcg gtacgctggt gagtgacgga tacagcgtgt
    21361 acaaatctct ggcggataat gaccccggta ttacatctgc ctgttgctgg agccacgcaa
    21421 ggcgcggttt cgcaaatctg tataaggcca gcagggagcc ccgtgcagcc atggcgctga
    21481 gaaaaatcgc gggtctttat cgcattgaga agttcatccg tgaacgtcct gtggaaaaaa
    21541 tacggcaatg gcgacaacgg tattcccggc cgatagtgaa tgacctgttt gcctggcctg
    21601 aagagcagga gccgtgctgt ccgcccgacg gaccactaaa caaagcgatt aattatatcc
    21661 tgaaccggcg cgatgaactg agctgcttcc tgagtgacgg agccgtgccc ctggacaaca
    21721 atatctgcga gcgtgctatc cgcccggtgg tcatggggcg gaaagcatgg ttattcgccg
    21781 gttcactgat ggccgggaac cgcgcggcac agataatgag ccttctggaa acggcaaaac
    21841 gtaatggtct ggagccgcat gcctggctga cagacgttct gacgcgtcta ccggagtggc
    21901 cggaagaccg actggaagag ttgctgcccc ttgagggatt taccttctcc gggtgagtga
    21961 tacctgccat caggtgttcg tgcagcgggc cataacccgc agtcgggagt tgaacgcctg
    22021 acgacaggaa atgagccaga gcagcgacca cgcgggctga cagtgagtca gaacctgaat
    22081 cagcccggag ccattcccgg ggctgtactt catcacctgc catcagtacc cgtgtcagta
    22141 cttcgcgacc tgacgcaatt acccgactca gttcttcaga acccgcctcc gataccagcc
    22201 tggcggtcgc ccctgtgcaa caaaataaat caaccatcgt cagacggtga tcaatcgtat
    22261 gcgctgccag tggctcgcct gaaaaatgcg tcaatatgag ttcgccggaa caatacctca
    22321 gttcttcaga acccgcctcc gataccagcc tggcggtcgc cccggcgcaa cagaataaat
    22381 caaccatcgt cagactgcga tcattcgcat gcatcgctga tgatgcgccc gaaaaacgcg
    22441 tcaatatgag ttcgccggaa tattactgac gacacggttt gtagttacgt tccttcggtg
    22501 agtgagtacc tgtgcgtcag ggtaacgcaa tcagttcagg tttagcgggg tgtcaagtcg
    22561 gtttggctaa ccgaccgcag aattaactgt acctttaaag gtcgcccccg atatatctgc
    22621 ctgtcagaca tatcgccgat tcggtatctc actacataaa actccttaaa aaacaatgcg
    22681 attctgttgg tacgaagctt tcagtaagtc agtccggaga agcgccagta cgatacagaa
    22741 tgaggttgaa aaaaacgttc acgtctggtc acttcttcaa atgttaaaaa acatctcgta
    22801 aagagccagt ttatgcgggt tcgttgagta gacgtgcgag cgtcactttg tcagctttaa
    22861 cgctgacgaa agtgacgccc gcacagcttc aaactccaca tgcacacgca agcgaccaaa
    22921 tttcaaatgg tcttatgccg tgcgcacagt cataagaagc gcatgcggaa agtcagaaaa
    22981 tatcttaaga cgcagatttt aaaggcgttt ttatgccagt ctgccgggaa tacacagtag
    23041 tccggttgtc cggaagcacc acggtaatcc gaaggaaaaa tcaacgtacc tcaatcaccg
    23101 tagtgatatc cgttcccaga ggctaaacgg atgtaccacc ataaaggcat cctctttcag
    23161 gctacgagga tattgcaatc acccgaaggc gtttctctca gattgctgaa acattggctg
    23221 gcagtcaccc gaaggcgtcc ctctcagact gctggaacat tggctggtcg caaaagcgac
    23281 agttaatgca aatacaggtt aacaaaaagg gaaatctgcc gtcaacccaa ccatatgaac
    23341 atgttgattt cattatgaat gtgatgttct aatataaatt aatcagtaac ttcataaaaa
    23401 gaatataaga aacagataat gcatgtattt acacacatat cagcacaatg atagattacc
    23461 accccagaaa cacgataaag gtataattat ggaattttcc gttatttttt tctgggttat
    23521 cactggtctt attaccatct ta
//
LOCUS       DAZUFB010000067        23450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZUFB010000067 DAZUFB010000000
VERSION     DAZUFB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.067"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..857)
                     /locus_tag="ACHY3M_003070"
     CDS             complement(<1..857)
                     /locus_tag="ACHY3M_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFS4317942.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHIYIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAA"
     gene            complement(1129..1290)
                     /gene="rsmS"
                     /locus_tag="ACHY3M_003071"
     CDS             complement(1129..1290)
                     /gene="rsmS"
                     /locus_tag="ACHY3M_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFS4317943.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(1304..1831)
                     /gene="priC"
                     /locus_tag="ACHY3M_003072"
     CDS             complement(1304..1831)
                     /gene="priC"
                     /locus_tag="ACHY3M_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFS4317944.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            1901..2278
                     /gene="ybaN"
                     /locus_tag="ACHY3M_003073"
     CDS             1901..2278
                     /gene="ybaN"
                     /locus_tag="ACHY3M_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFS4317945.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            2431..2982
                     /gene="apt"
                     /locus_tag="ACHY3M_003074"
     CDS             2431..2982
                     /gene="apt"
                     /locus_tag="ACHY3M_003074"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4317946.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            3111..5042
                     /gene="dnaX"
                     /locus_tag="ACHY3M_003075"
     CDS             3111..5042
                     /gene="dnaX"
                     /locus_tag="ACHY3M_003075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4317947.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    4375..4439
                     /gene="dnaX"
                     /locus_tag="ACHY3M_003075"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            5095..5424
                     /gene="ybaB"
                     /locus_tag="ACHY3M_003076"
     CDS             5095..5424
                     /gene="ybaB"
                     /locus_tag="ACHY3M_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4317948.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            5424..6029
                     /gene="recR"
                     /locus_tag="ACHY3M_003077"
     CDS             5424..6029
                     /gene="recR"
                     /locus_tag="ACHY3M_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4317949.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            6139..8013
                     /gene="htpG"
                     /locus_tag="ACHY3M_003078"
     CDS             6139..8013
                     /gene="htpG"
                     /locus_tag="ACHY3M_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFS4317950.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            8194..8838
                     /gene="adk"
                     /locus_tag="ACHY3M_003079"
     CDS             8194..8838
                     /gene="adk"
                     /locus_tag="ACHY3M_003079"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4317951.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            9074..10036
                     /gene="hemH"
                     /locus_tag="ACHY3M_003080"
     CDS             9074..10036
                     /gene="hemH"
                     /locus_tag="ACHY3M_003080"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFS4317952.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(10033..10992)
                     /gene="aes"
                     /locus_tag="ACHY3M_003081"
     CDS             complement(10033..10992)
                     /gene="aes"
                     /locus_tag="ACHY3M_003081"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFS4317953.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            11144..12448
                     /gene="gsk"
                     /locus_tag="ACHY3M_003082"
     CDS             11144..12448
                     /gene="gsk"
                     /locus_tag="ACHY3M_003082"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFS4317954.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(12581..14257)
                     /gene="ybaL"
                     /locus_tag="ACHY3M_003083"
     CDS             complement(12581..14257)
                     /gene="ybaL"
                     /locus_tag="ACHY3M_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFS4317955.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(14495..15715)
                     /gene="fsr"
                     /locus_tag="ACHY3M_003084"
     CDS             complement(14495..15715)
                     /gene="fsr"
                     /locus_tag="ACHY3M_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFS4317956.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            15933..17585
                     /gene="ushA"
                     /locus_tag="ACHY3M_003085"
     CDS             15933..17585
                     /gene="ushA"
                     /locus_tag="ACHY3M_003085"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS4317957.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(17622..18101)
                     /gene="ybaK"
                     /locus_tag="ACHY3M_003086"
     CDS             complement(17622..18101)
                     /gene="ybaK"
                     /locus_tag="ACHY3M_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFS4317958.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(18305..19099)
                     /gene="ybaP"
                     /locus_tag="ACHY3M_003087"
     CDS             complement(18305..19099)
                     /gene="ybaP"
                     /locus_tag="ACHY3M_003087"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFS4317959.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            19237..19578
                     /gene="ybaQ"
                     /locus_tag="ACHY3M_003088"
     CDS             19237..19578
                     /gene="ybaQ"
                     /locus_tag="ACHY3M_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS4317960.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(19692..22196)
                     /gene="copA"
                     /locus_tag="ACHY3M_003089"
     CDS             complement(19692..22196)
                     /gene="copA"
                     /locus_tag="ACHY3M_003089"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFS4317961.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            22458..23390
                     /gene="glsA"
                     /locus_tag="ACHY3M_003090"
     CDS             22458..23390
                     /gene="glsA"
                     /locus_tag="ACHY3M_003090"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4317962.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     ATFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5742 a         6282 c         6182 g         5244 t
ORIGIN      
        1 gccgccagac ggtcgcggaa cgcccgtccc cactcagtct tactggtggc taccattgcg
       61 ggcatataca acgccctgcg aagcgacaca tgtccggcct tactcatccg gctcgcccct
      121 ctcacactgc tacctgattc ataacgccgt ggtgtcagac ccgcaaaagc ggcgaactgt
      181 ctggcatggg cgaagcggtc cttcagaccg atataagcca gcaataccgc agatgttttc
      241 tctccgatac ccgggatgct ttccagcagt ttcctgcggt gtttcatatc cggatcatcg
      301 tctgtcaggt cttttatctg cttctcaaga cgcttcagct ctgcttcaag ccacagaagg
      361 tgagcatcaa tgctcggtct ctggacttcc cgcgccgttt cagtgcgatt cagttcctgc
      421 gtgtgcatat ctgtcagcgc ctggtggcgg actaccaggg cacgcaacgc gcgttcaagc
      481 gggtgaggcg cttcccaggc tgcagggcgc ttctgacgac agaactctgc cagcatgcgc
      541 gcatccacgg tatcagtctt gttacgcagt ccttcactct gagcgaaagc tttacccagc
      601 gcaggattaa tgactgacac tatgtagcca gcatcgtaaa ggcactcagc gacaggttcc
      661 atataggtgc cggtcgcttc gatgtagata tgcgcatggt caatcttgtg acctttcagc
      721 cagctcacca gctcatcgtg ccctttagtg gtgttagcga attttttggt gcgatgacga
      781 ccatcaggac gcaacacatc gacatccagt ttctctttag cggtgtcgat accgatataa
      841 tgaagttcat gttccatagt gaaaccaacc ttgcaaatac ggattaccgg gaaaaccggt
      901 ccatgatact gtccggttta tcactttggg agaaaggcag tccgctgcat aaatctacgc
      961 aacaggttag aacccaaggc ccgatacggc atgcggactg caggcagaga aaactggccg
     1021 attttctctg cactggaggg ataatacaag gcctgataag cgtagcgcat caggcagttt
     1081 tgcgtttgcc cacaatctta ggggacattt agcggcccca tttatttctc acttttccgc
     1141 ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
     1201 cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga cagccagttt
     1261 gacatcgtca ggggcatttt ccagtgacat actctctcca ttgctagcgg gttaagcgcg
     1321 ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg
     1381 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
     1441 ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
     1501 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg
     1561 aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt
     1621 gcggcagctg ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcat
     1681 cgagacaagc ctgcagtgtt gtcgcacggg tttgaaaaag gtgtctgtca aaacgggcac
     1741 ttagcgtggc aaattgcgct accggggcac aacgctgacg cagcgtagcg agctgtcctt
     1801 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
     1861 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
     1921 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
     1981 gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca
     2041 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
     2101 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
     2161 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
     2221 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
     2281 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
     2341 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
     2401 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
     2461 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
     2521 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
     2581 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
     2641 ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa
     2701 ctgccgcgtg aaaccatcag tgaaacttac gacctggaat acggcaccga tcagctggag
     2761 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
     2821 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
     2881 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
     2941 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
     3001 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
     3061 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
     3121 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
     3181 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
     3241 ccggtacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
     3301 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
     3361 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata
     3421 cccgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc
     3481 tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg
     3541 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg
     3601 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
     3661 gccatcaact tgagcacatc ctcaacgaag aacatatcgc tcacgagccg cgggcgttgc
     3721 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
     3781 cgattgccag cggtgacggc caggtttcaa cccaggcagt cagtgcgatg ctgggtacgc
     3841 ttgacgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
     3901 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
     3961 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
     4021 acgacatggc cgccattgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
     4081 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
     4141 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccacgtatgc
     4201 ctctgcctga gccagaagtg ccacgccagt cctttgcgcc cgtcgcgcca acggcagtaa
     4261 tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca ccgactgtac
     4321 cgctcccgga aactaccagc caggtgctgg cggcgcgcca gcagttgcag cgcgtgcagg
     4381 gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgg ccggtgaata
     4441 acgctgcgct ggaaagactg gcttcggtca ccgatcgcgt tcaggcgcgt ccggtgccat
     4501 cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg ctggaaggct accactccag
     4561 tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct gaaaaaagcg ctggaacatg
     4621 aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc cattgagcgc gacgcgtggg
     4681 cggcacaggt gagtcaactt tcgctaccaa aactggtcga acaggtggcc ttaaatgcct
     4741 ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg ctcctctcag cggcatttga
     4801 acaaccgcgg tgcacagcaa aaactggctg aagcgttgag cacgttaaaa ggttcaacgg
     4861 ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg gagtggcgtc
     4921 aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg gataataata
     4981 ttcagaccct gcgtcggttc ttcgatgcgg agctggatga agaaagtatc cgccccattt
     5041 gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa acctatgttt
     5101 ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga aaaaatgcag
     5161 aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc aggtctggta
     5221 aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc gagcctgctg
     5281 gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga cgcagcacgt
     5341 cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat gcagctgccg
     5401 cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag cttatggaag
     5461 cactgcgctg tctgccgggc gttggcccga agtcggcgca gcgtatggcg ttcacgctgc
     5521 ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg gcgatgtcgg
     5581 aaatcggcca ctgcgccgat tgccgcactt tcaccgaaca ggaagtctgt aacatctgtt
     5641 cgaatccgcg tcgtcaggaa aacggtcaaa tctgtgtggt ggagagtccg gcggacatct
     5701 acgccattga gcagacgggg cagttttcag gccgttattt tgtgttgatg gggcacctgt
     5761 caccgctgga cggcatcggt ccggatgata tcggccttga tcgtctggaa cagcgtctgg
     5821 cagaggaaaa aatcactgaa gtgatcctcg ccaccaatcc cacggttgaa ggtgaagcta
     5881 ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagccagc cgaatcgctc
     5941 atggcgtacc ggttggcggc gaactggaaa tggtcgatgg caccacgctg tcacactccc
     6001 ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc tgctctcgct
     6061 tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct taaaatggca
     6121 ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc agaagtgaaa
     6181 cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt cctgcgtgag
     6241 cttatctcta acgcctccga tgcggcggac aagctgcgtt tccgtgcgct atctaacccg
     6301 gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa agacaagcgc
     6361 acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat tgaccatctg
     6421 gggactatcg ctaaatccgg taccaaatca ttcctcgaat ccctgggttc tgaccaggcg
     6481 aaagacagcc agctgatcgg tcagtttggt gttggtttct actctgcgtt tatcgtggcc
     6541 gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg cgtcttctgg
     6601 gaatcggctg gcgaaggtga atataccgtt gccgacatca ccaaagaaga tcgtggtact
     6661 gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg gcgcgtgcgt
     6721 tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga aaaacgcgaa
     6781 gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca acaaagcgca ggcgctgtgg
     6841 actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa acacatcgcc
     6901 cacgacttta atgatccgct gacctggagc cacaaccgcg ttgaaggtaa gcaggagtac
     6961 accagcctgc tgtacatccc gtcccaggct ccgtgggata tgtggaaccg tgatcataaa
     7021 cacggcctga aactgtacgt ccagcgtgtg ttcatcatgg acgacgcaga acagttcatg
     7081 ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc gctgaacgtt
     7141 tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc gctgaccaag
     7201 cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggacaaata ccagaccttc
     7261 tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc taaccaggaa
     7321 gcgatcgcca aactgctgcg ttttgcttct acttataccg attcttctgc gcagaccgtg
     7381 tctctggaag actacgtttc ccgaatgaaa gaagggcagg agaaaatcta ctacatcact
     7441 gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg taagaaaggc
     7501 atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta tctgactgag
     7561 ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga aaaactggct
     7621 gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt catcgaccgt
     7681 gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg tctgaccgat
     7741 acgccagcga tcgtttcgac cgacgcggac gaaatgagca ctcagatggc gaaactgttc
     7801 gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa cccggatcac
     7861 gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga gtgggtagaa
     7921 ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc tggaagatcc gaacctgttt
     7981 attcgtcgta tgaaccagct gctggtttcc tgatgtcatg ccggatgacc tttgtgtcat
     8041 ccggcatttt tcttttcatc atctgcactt tccgcaaatt atctcgccat taaccgtttc
     8101 agcctcaggt gcctttcttg aggcaatcgc ctgttggtgg tatcgtttat cgctttttca
     8161 aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct tggcgctccg
     8221 ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat tccgcaaatc
     8281 tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg taaacaagca
     8341 aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc gctggttaaa
     8401 gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt cccgcgtacc
     8461 attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt tctggaattc
     8521 gacgtaccgg acgaactgat tgttgatcgt atcgtaggcc gccgcgttca tgcgccgtct
     8581 ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggcaaaga cgacgttacc
     8641 ggtgaagaac tgactacccg taaagacgat caggaagaaa ccgtgcgtaa acgtctggtt
     8701 gaataccatc agatgactgc accgctgatc ggctactact ccaaagaagc ggaagcgggt
     8761 aacaccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg cgctgatctg
     8821 gaaaaaatcc tcggctaatt cgaaagcgcg cacggacagt cccctcgccc cctcggggag
     8881 agggttaggg tgaggggaac aggcccgcac aagcaaactt atcagcaatc tcaggccgga
     8941 tattcattcg gccttttaca aaaacctcaa tctgcgctat tcctttttct gatttgacct
     9001 ctcacaacaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
     9061 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
     9121 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
     9181 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
     9241 gcgctcgccg cgtgtggcga agatgtacgc ctcggtctgg atggaagacg gctcgccgct
     9301 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgcccgt
     9361 agcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
     9421 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
     9481 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
     9541 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
     9601 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
     9661 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
     9721 cgaactggct tccgcactgg ggatggcacc ggaaaaagtg atgatgacct ttcagtcgcg
     9781 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
     9841 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
     9901 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgccggcg ggaaaaaata
     9961 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    10021 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    10081 ggcggttttc atcattcgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    10141 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    10201 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    10261 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    10321 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    10381 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    10441 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    10501 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    10561 ataacaacaa gcagccacaa tttcctctat cgcttgcgga aaacgcgctt caggtgaaag
    10621 ggggtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    10681 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    10741 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    10801 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    10861 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    10921 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    10981 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    11041 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    11101 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    11161 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    11221 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    11281 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    11341 ggtgattgag gatgacgtag ccgaagcgct ttatcaggag ctaaaacaga aaaacctgat
    11401 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    11461 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    11521 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    11581 tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    11641 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    11701 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    11761 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    11821 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    11881 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    11941 tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt gcaccgccgg
    12001 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    12061 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    12121 ccacaaggat tgccagaatc cgctgcgtgt atattcgcac attgcgccgt acatgggcgg
    12181 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    12241 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    12301 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    12361 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttaccggagc gtgaagacag
    12421 cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    12481 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    12541 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacctcac
    12601 ccgccggtgg cgtttccagc agttccagca tggtacgggc gatttcacgc tcgcccatca
    12661 ctacctgatt cgcaccacgt tcggtgatat acgccacttc atcgtcataa tgggcgcggg
    12721 caataatctc aatatccgga tttttcgcgc gggcagatgc cacaatctca cccgcttcat
    12781 aaccgttggg aatcgtcagg atcagccatt ttgcacattc cagatgcgcc agttgcataa
    12841 tttcttcgtt cgccgcattg cccaatactg cgcggacccc gcgctctcgc agctcatcaa
    12901 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    12961 ccagcaggct gcctacacga ccgtaaccca ccagtagcgc atggttgcaa atatccactg
    13021 ggatctgctt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    13081 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    13141 cccctgccag tactaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    13201 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    13261 tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc ggctaacgac ttaccaaaca
    13321 gaataatcgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    13381 acaacatccc gacggagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcagcgtat
    13441 cgtgggcggc acggtgactc agttcagact cgttcagtac catcccggca aagaacgcac
    13501 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    13561 ccagcaccga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    13621 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc actttgccga
    13681 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    13741 ctgccactgc gggcagcaac accagcgtca gaaccattac caggtcttcc acaatcaacc
    13801 aaccgatggc gatttgccca cgctgactgt caattaattg ccgttcttca agtgcgcgca
    13861 gtaacaccac ggtactggcg gtggaaagac ataaaccgaa cacgataccg gtcattaacg
    13921 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    13981 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    14041 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    14101 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    14161 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    14221 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    14281 attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    14341 gatgatgttc atgaataatt attgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    14401 cacggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    14461 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    14521 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    14581 tactggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc ataccaaaag
    14641 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagcagc tcctgagcgt
    14701 aaaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    14761 ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    14821 agccccaaat cacatatttc cgcccaattt tatcccctac aggcccgccg atcaccgtac
    14881 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg agcattctgg atagataatc
    14941 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    15001 tcgagaaaat gaggattaac agaatgctga ccgccagtac cactttgttg cgcggcagtg
    15061 gattgataat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    15121 ggctgatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    15181 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    15241 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    15301 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg
    15361 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    15421 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    15481 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    15541 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    15601 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    15661 tggtcgttga cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa
    15721 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    15781 acatacctgt atgcgtcgcc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    15841 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    15901 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    15961 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    16021 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    16081 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    16141 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    16201 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    16261 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    16321 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    16381 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gtcattgggc
    16441 tgacaaccga tgacacagca aaaattggta acccggaata cttcactgat gtcgaatttc
    16501 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    16561 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    16621 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    16681 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    16741 tgccgggtac gccatgcaag ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    16801 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcaaa atgaaaatgg
    16861 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    16921 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    16981 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcgaa accaatggtc
    17041 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    17101 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    17161 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    17221 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    17281 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    17341 cgaaagacgg cagactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    17401 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    17461 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    17521 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    17581 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    17641 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    17701 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    17761 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    17821 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    17881 tttttaagat ccagttgacc ggcgaccggc gtaacggcca cggcaaggtg tttcatatca
    17941 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    18001 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    18061 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    18121 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    18181 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    18241 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    18301 ttttttagcg caacatttgc ggtaaattcc cttctccata caggtgtagt gcaccgaccg
    18361 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    18421 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atttcattat
    18481 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcattggtgt
    18541 gccagtgggt cagtgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    18601 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    18661 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    18721 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    18781 tgcccatctc ctggctaatg tgctgcagat tttggagttg ctcctcgcta atgcgctctt
    18841 ccagcgcctc gcaggcaggc aaattggcaa aaggcgtatc gctggtggaa acatccgcct
    18901 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    18961 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg
    19021 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    19081 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggggag
    19141 ggatggtgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatattctt
    19201 gtatcgctat tcacagggaa gcaacagtta aaaactatga aacaggcaac aagaaaaccg
    19261 acgacacccg gagatattct tctctatgaa tatctggtac cgctcgattt gaaaatcaat
    19321 gagttagcag agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat
    19381 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    19441 ttttggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    19501 caggaagaat tgggacggat tgaaacagtg gctgaatact tggcacgccg tgaagagcgt
    19561 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttt
    19621 acgccgcatc cggcagtcat gcgtcgatgc caaatgtgcc accctaaagc agcgcatccg
    19681 caatgacata cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg
    19741 gtaatcgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
    19801 aacggccaca aaataccggc ggcgaccgga ataccgatac tgttgtagat aaaggcaccg
    19861 agcaggttct gcttcatgtt gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg
    19921 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    19981 ccccccatcg caataccgac atccgcctgg gccagcgctg gcgcatcgtt aatgccgtcg
    20041 cccaccattg ccacctgacg tccttcactt tgcaggcgtt taatcgcttc ggctttaccg
    20101 tccggcagca ccccggcgat cacttcatca atcccggctt ctttggcgat cgcattggcg
    20161 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc
    20221 agcgccgcca cgctatcact acgcaatgga tcgcgtactg ccagcagggc taccgctttc
    20281 ccgtcaaccg ccagcagcac aggcgttgcc ccttgcgatg cctgagcagt aatctccgct
    20341 tcgatagctt tggtaccaac ctgttgctca tttaacagcg cctgattgcc cagcaataac
    20401 gcatgacctt cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    20461 tgcggcagct gcatatcacc tgctttatcg aggatcgccc gtgccagcgg atggctggaa
    20521 ccttgctcca gtgccgccgc cagacgcaat gcctgcgctt catcaacatc agcaaacgtt
    20581 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg
    20641 gtatcgagtg tactggcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
    20701 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga
    20761 caggcgataa tcagtaccgt ggtggcaatc accagggtat aaacaatttg cggtgccgga
    20821 ccaaagaagt accagattgc cgcactgacc agcgcaatca ccaccaccac aggtacaaat
    20881 acggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
    20941 accatgcgaa tgatccgtga cagcgtggta tggctgccaa ccgcactggc acgaaacagc
    21001 acgctaccgt cctgtaccac tgtcccggca tggacgcttt cgccttcgcc tttttgctgc
    21061 gggattggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
    21121 ccatctaccg gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttcc
    21181 gccagaggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa
    21241 tcgagtaatt tctccagcgc cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg
    21301 ccgagattga tcagaccgat aatcatcgcg ctggcttcgt aataaagatg tcgcgcttcc
    21361 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacgccagta
    21421 cccagcgcca ccagcgtatc catcgtcgct gcaccgttca gcaggctttt ccatgcactg
    21481 cggtaaaaat ggccgccggc gaaaaccatc accgccaggg ttatcaggcc gataaccaac
    21541 cacaggctgc ggttgtcagc ggtgaccatc atgttatcgc caatcatccc ccagaccatc
    21601 accgggatac ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagca
    21661 acggcggttt cctgctggcg ctcacggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
    21721 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    21781 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
    21841 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    21901 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    21961 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    22021 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
    22081 gtgatagaca catccgcttg ttccacatcc gggcgctgtt cgagactttc tttcacgcgt
    22141 ttaacgcagt gaccgcagga caggccgtcc agggtcaggt cgataatttg tgacataaaa
    22201 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    22261 ggtcaagaaa ttaataaacc aggcgagtaa aagtccgtaa agattaaaaa attggctcaa
    22321 tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa
    22381 gttaaatcaa gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa
    22441 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    22501 acacccaatt tcactcactt aacggcggac aaaatgccga ctacattccc tttctggcga
    22561 atgtacctag tcaactggca gcagtggcta ttgtgacctg cgatggcaac atctatcgcg
    22621 cgggtgatag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc
    22681 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
    22741 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaacca ctgtcgccac
    22801 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat attgaacaac
    22861 gctggcagcg aattttacat atccaacagc aactggctgg tgagcaggta gcgctctctg
    22921 atgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc
    22981 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    23041 agtgctccac gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg
    23101 gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc
    23161 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg
    23221 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg
    23281 gaattgccac gttctcacca ccgctggacg aagaaggcaa cagtgttcgc ggtcaaaaaa
    23341 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    23401 cacggaaggt aataacggta acaaacctct cggtctatgg aacgtcgttt
//
LOCUS       DAZUFB010000068        23314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZUFB010000068 DAZUFB010000000
VERSION     DAZUFB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.068"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1165)
                     /gene="ydcO"
                     /locus_tag="ACHY3M_003091"
     CDS             complement(<1..1165)
                     /gene="ydcO"
                     /locus_tag="ACHY3M_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFS4317963.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1257..1793
                     /gene="sutR"
                     /locus_tag="ACHY3M_003092"
     CDS             1257..1793
                     /gene="sutR"
                     /locus_tag="ACHY3M_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFS4317964.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1866..3827
                     /gene="rlhA"
                     /locus_tag="ACHY3M_003093"
     CDS             1866..3827
                     /gene="rlhA"
                     /locus_tag="ACHY3M_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFS4317965.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3919..4149)
                     /gene="yncJ"
                     /locus_tag="ACHY3M_003094"
     CDS             complement(3919..4149)
                     /gene="yncJ"
                     /locus_tag="ACHY3M_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFS4317966.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4371..4547
                     /gene="hicA"
                     /locus_tag="ACHY3M_003095"
     CDS             4371..4547
                     /gene="hicA"
                     /locus_tag="ACHY3M_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFS4317967.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4593..5009
                     /gene="hicB"
                     /locus_tag="ACHY3M_003096"
     CDS             4593..5009
                     /gene="hicB"
                     /locus_tag="ACHY3M_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFS4317968.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5088..6494
                     /gene="ydcR"
                     /locus_tag="ACHY3M_003097"
     CDS             5088..6494
                     /gene="ydcR"
                     /locus_tag="ACHY3M_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4317969.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLVQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6739..7884
                     /gene="ydcS"
                     /locus_tag="ACHY3M_003098"
     CDS             6739..7884
                     /gene="ydcS"
                     /locus_tag="ACHY3M_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFS4317970.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7902..8915
                     /gene="ydcT"
                     /locus_tag="ACHY3M_003099"
     CDS             7902..8915
                     /gene="ydcT"
                     /locus_tag="ACHY3M_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4317971.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8916..9857
                     /gene="ydcU"
                     /locus_tag="ACHY3M_003100"
     CDS             8916..9857
                     /gene="ydcU"
                     /locus_tag="ACHY3M_003100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4317972.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9847..10641
                     /gene="ydcV"
                     /locus_tag="ACHY3M_003101"
     CDS             9847..10641
                     /gene="ydcV"
                     /locus_tag="ACHY3M_003101"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4317973.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10663..12087
                     /gene="patD"
                     /locus_tag="ACHY3M_003102"
     CDS             10663..12087
                     /gene="patD"
                     /locus_tag="ACHY3M_003102"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFS4317974.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGEVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            12297..13106
                     /locus_tag="ACHY3M_003103"
     CDS             12297..13106
                     /locus_tag="ACHY3M_003103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFS4317975.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
     gene            complement(13508..13603)
                     /gene="yncL"
                     /locus_tag="ACHY3M_003104"
     CDS             complement(13508..13603)
                     /gene="yncL"
                     /locus_tag="ACHY3M_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFS4317976.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13798..13971
                     /gene="ortT"
                     /locus_tag="ACHY3M_003105"
     CDS             13798..13971
                     /gene="ortT"
                     /locus_tag="ACHY3M_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFS4317977.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            14057..14290
                     /gene="ydcY"
                     /locus_tag="ACHY3M_003106"
     CDS             14057..14290
                     /gene="ydcY"
                     /locus_tag="ACHY3M_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFS4317978.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(14291..14740)
                     /gene="ydcZ"
                     /locus_tag="ACHY3M_003107"
     CDS             complement(14291..14740)
                     /gene="ydcZ"
                     /locus_tag="ACHY3M_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4317979.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(14737..15255)
                     /gene="mddA"
                     /locus_tag="ACHY3M_003108"
     CDS             complement(14737..15255)
                     /gene="mddA"
                     /locus_tag="ACHY3M_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFS4317980.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            15436..16473
                     /gene="curA"
                     /locus_tag="ACHY3M_003109"
     CDS             15436..16473
                     /gene="curA"
                     /locus_tag="ACHY3M_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFS4317981.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            16671..17336
                     /gene="mcbR"
                     /locus_tag="ACHY3M_003110"
     CDS             16671..17336
                     /gene="mcbR"
                     /locus_tag="ACHY3M_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFS4317982.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(17372..19474)
                     /gene="pqqU"
                     /locus_tag="ACHY3M_003111"
     CDS             complement(17372..19474)
                     /gene="pqqU"
                     /locus_tag="ACHY3M_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFS4317983.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSTAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            19716..20777
                     /gene="yncE"
                     /locus_tag="ACHY3M_003112"
     CDS             19716..20777
                     /gene="yncE"
                     /locus_tag="ACHY3M_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFS4317984.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20892..22390)
                     /gene="ansP"
                     /locus_tag="ACHY3M_003113"
                     /pseudo
     CDS             complement(20892..22390)
                     /gene="ansP"
                     /locus_tag="ACHY3M_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
     gene            22656..23273
                     /gene="yncG"
                     /locus_tag="ACHY3M_003114"
     CDS             22656..23273
                     /gene="yncG"
                     /locus_tag="ACHY3M_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFS4317985.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         6030 a         5593 c         5941 g         5750 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtcgcgctca ttatgcagcg cctgatacaa actgccgcta atggtactta
      181 acagcgccag acctgccagc atctggatcc aacttacggg cagggcagcc atcatcccgg
      241 taatagcact accaaacaga cctgcgatca aataaaaaat gcctgcaacg gcagctgcca
      301 gccaacgttg atctttatcc ggatgcgctt ccgggctttg gcaaatagcc gcggtgattg
      361 ccgcaatacc gacggaataa acgccgaaag gggaaaaaac cagtgccagc aatccagtaa
      421 atacaattaa tggcgaaaca ggagccgaat acccggctgc tttcattgct gcgatacccg
      481 gtgcgttttg cgatgccatc gtcaccagaa aaagggggag tgcaacgctc aggctgtgag
      541 caaacgaaaa atcaggggta atataagtgg ggagaacggg tttaaagaca acatcagttg
      601 tgacaacgtc accttgcgcg ataacgatca cgatcccaat aatcatcgcg gcaattaccg
      661 catagcgcgg cgcaacggcc ttggttgcca gccataccag caacatactt ccacacaacg
      721 taaattgacc gtccagactg gcaaacgcct gtaaaccaaa gcgtaataaa atcccggcaa
      781 gcattgccgc cgcaagcgag tgcggaataa tgcgcatcag acgagcaaag agtcccgtta
      841 tgccgcagag gactattaac gcgttggtga caataaaaac gccgatggct tcgttaagtg
      901 ttagtccctg caatccggtg accaacaaag ccgcgccagg cgttgaccat gcggtgagaa
      961 caggtacgcg ataccataat gtcagagtca gcgtactgac gcccattgcc agccccagcg
     1021 ccgtcatcca gccagagatt tgtgcagtgg tggctccggc gacaatcgct gcttgccaga
     1081 ttattgccgc tgaactggcg tagccaatta ataccgccag aaaccccgcc agtagcgtgg
     1141 gtggaggaat agaaaacaga cgcatagtca cctcgtgcgt tatagcgtac aggcaccgta
     1201 gcatttgtcc gttataacgc acaagtgata aacttccgtt ttgccggagg agtcgcatgg
     1261 aaaatctcgc tcgcttttta tccaccacac ttaaacaact acgccagcag cgcggctgga
     1321 gtctttcgcg actggcagaa gcgacgggcg tttctaaagc aatgttaggg cagattgagc
     1381 gtaatgagtc cagtccgacg gtcgcgacat tatggaaaat tgccaccggg ttgaacgtac
     1441 cattttcaac atttatttct ccgccgcagt ccgctacgcc ttcagtttat gatccacaac
     1501 agcaggcgat ggtgatcact tcgctgtttc cttatgatcc acaactctgc ttcgaacact
     1561 tctcgattca gatggcatct ggtgcaataa gcgaatcgac gccccatgag aagggggtaa
     1621 tcgaacatgt cgtcgtcatt gatggacaac ttgatctgtg cgttgatggg gaatggcaga
     1681 cccttaattg tggggaaggc gttcgatttg ctgcagacgt cacgcatatc tatcgtaatg
     1741 gtggggagca aaccgtacat tttcattccc tcatccatta cccgcgcagt taagcgggaa
     1801 aactatttcg caacgtcgcg cttgtggcta aaatagccgc catttttcag ctactggata
     1861 agaatgtgac cgtatcttct catcgacttg aactgttaag cccggcacgc gatgccgcca
     1921 ttgcccgcga agctattttg cacggtgccg atgctgttta tatcggcggc cctggttttg
     1981 gtgcccgtca taatgccagt aatagcctga aagatattgc cgagctggtg ccgtttgccc
     2041 atcgttatgg tgcaaaaatt ttcgtcacgc ttaacaccat tttgcatgat gatgagctgg
     2101 aacccgcgca acggctgatt accgacctct accagaccgg tgtcgatgcg ctgattgttc
     2161 aggatatggg gattctggaa cttgatattc cgccgattga actgcacgcc agtacgcagt
     2221 gcgacattcg tacagtagaa aaagcgaagt tcctctctga tgttggcttc acgcagattg
     2281 tgctggcgcg agagctgaat cttgagcaga tccgcgcgat tcaccaggcc acggacgcga
     2341 ccattgaatt ctttattcat ggggcactgt gcgtggccta ttcgggtcag tgctacattt
     2401 ctcatgcgca aacagggcgt agcgccaacc gtggtgattg ctcgcaggcg tgccgtttgc
     2461 catacacatt gaaagacgat caggggcggg tggtttccta tgaaaaacat ctgctgtcga
     2521 tgaaagataa cgatcagact gccaacctcg gcgcgctgat tgatgctggc gtacgctcct
     2581 tcaagattga agggcgttac aaagatatga gctacgtgaa gaatatcacc gcccattatc
     2641 gccagatgct tgatgccatt attgaagaac gtggcgatct ggcgcgcgct tcatcaggtc
     2701 gtactgaaca tttctttgtt ccatcgacgg aaaagacttt ccaccgtggt agcacagatt
     2761 attttgtgaa tgcccgtaaa ggcgatattg gcgcgttcga ttcgccgaaa tttatcggcc
     2821 tgccggtagg cgaagtattg aaagtggcga aagatcatct cgatgttgcc gtcaccgagc
     2881 cactggcaaa tggtgatggc ctgaacgtga tgattaaacg tgaagtcgtc ggttttcgtg
     2941 ccaatacggt cgagaaaacc ggagaaaatc agtaccgcgt ctggcccaat gaaatgccag
     3001 cagatttgca caaaattcgt ccacatcacc cactaaaccg taatcttgat cataactggc
     3061 agcaggcact gacaaaaacc tccagcgaac gtcgggtggc ggtagacatt gaactgggcg
     3121 gctggcagga acaactgatt ctgaccctca ccagtgaaga gggtgtcagc atcacgcata
     3181 cgctggacgg gcagttcgac gaagccaata acgccgaaaa agcaatgaac aatctgaagg
     3241 atggtctggc aaaactgggg caaaccctct attacgcccg cgatgtgcaa attaatttgc
     3301 cgggggcgct gtttgtacca aacagtctgt taaaccagtt ccgccgtgaa gctgctgaca
     3361 tgctggatgc tgcgcgtctt gccagttacc agcgcggcag ccgtaaaccg gttgctgatc
     3421 ctgcgccggt ttatccgcaa acgcatctga gtttcctcgc gaacgtatac aaccagaaag
     3481 cgcgtgaatt ttatcaccgc tatggtgtgc agctgattga cgcggcgtat gaagcacatg
     3541 aagagaaggg cgaagtcccg gtgatgatca ccaagcattg tctgcgcttt gcctttaatc
     3601 tgtgcccgaa acaggcgaaa ggcaatatca aaagctggaa ggcgacgcca atgcaactgg
     3661 ttaacggcga tgaagtatta acgctaaagt ttgattgccg cccatgcgag atgcacgtca
     3721 ttggcaaaat caaaaatcac atactgaaaa tgccgttacc gggaagcgta gtggcatccg
     3781 taagtccgga tgagctgctg aaaacattgc ctaagcgaaa agggtaaaac gccagttttc
     3841 tggttactca caactcattg aatctgcatg atattgcctg ccgggtaagg cgtctcgccg
     3901 catccggcaa caaatgactc aacgttgtcc cgcttctggt ttgcgcgatt tttgccagta
     3961 aaaatgctcg cgcaaacctt ccgccgactc ttccgccaca gcacgcaatt catcgctgtc
     4021 cgcttcatga cgcagctgat gatccacatt ctttacccac acaaattcat gtcctttgtg
     4081 ccctgccatg agttgtcctg agaacagcgc acacgttaat aagacaaccg ataacgcctt
     4141 cgtaaacatc ctgccacctt tttcttacct tttgccgcta tgatgccgat cgtttcttga
     4201 ggttattatt cagttttgca aattagcgca aagaaattct ggaatcttcc ttcctgattt
     4261 tgcatggcat tctgccgttg cggcgattta gtgctatttt cgagcaaatt acacacggag
     4321 gtaaacacta atgtttactt tgttgatata ctcagcggca gggaggcgat gtgaaacaaa
     4381 gcgagttcag acgttggctc gaatctcagg gcgtcgatgt agcgaatggc agcaaccatt
     4441 tgaaactcag gtttcatggg aggcgcagtg tcatgccgcg tcacccctgc gatgagatta
     4501 aagaaccatt gcgtaaagca atcctgaaac aactcggttt gagttaatcg ccaattaaaa
     4561 aggttaatga catgcgaggg acagtcgaaa ttatgcgtta tcccgtcact cttacacccg
     4621 cgccggaagg cggttatatg gtttcttttg tggatatccc tgaagcgttg acgcagggcg
     4681 aaactgtcgc tgaagcgatg gaagcggcaa aagatgcttt actgaccgca tttgattttt
     4741 attttgaaga taacgagctt atcccgttac cttcgccatt aaatagtcat gatcacttta
     4801 ttgaagtacc tttgagcgtc gcctctaaag tattgctgtt aaatgctttt ttacagtcag
     4861 aaatcactca gcaagagtta gccaggcgaa ttggcaaacc taaacaggag attactcgcc
     4921 tatttaactt gcaccatgcg acaaaaatcg acgccgtcca gcttgcggca aaggcgcttg
     4981 gcaaagagtt atcgctggtg atggtttaat tacagttaac gaaaagttgt catttttaac
     5041 aactgatata gactgccgaa tcatctgcac ataattacga ttcgataatg aaaaaatacc
     5101 agcagcttgc agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa cccggcgatc
     5161 gtttgccttc gttgcgtgac caggtggcgc tttcaggcat gagctttatg actgtcagcc
     5221 atgcctatca gttgctcgaa agtcagggat atattatcgc acgaccgcag tcgggttatt
     5281 acgttgcgcc acaggcaata aaaatgccga aagcgccagt cattccagtc actcgagatg
     5341 aagcagtcga tatcaacact tatatttttg atatgttgca ggccagtcgc gatccgtcgg
     5401 tcgttccgtt tgcctcggcc tttcccgacc cgcgactttt ccccctccaa caactaaacc
     5461 gctcgctggt gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa aacttaccgc
     5521 caggaaacgc agaactgcgt caggctattg ctcgtcgcta tgccttacag ggcatcacca
     5581 tttctcctga tgaaattgtc attactgccg gggcgttaga ggcattaaac ctcagtttgc
     5641 aagcggtaac tgaaccgggc gattgggtga tagtagagaa tccttgtttc tatggcgcgt
     5701 tgcaggcgct ggaacggcta cggctgaaag cgttatcggt ggcgacggat gttaaagaag
     5761 ggatagatct tcaggcgttg gaactggcgt tgcaggagta tccggtgaaa gcgtgctggc
     5821 tgatgactaa tagccagaat ccactcggat ttaccttaac gccgcaaaaa aaagcacaac
     5881 tggtggcgtt actcaatcag tacaacgtaa cgctgattga agatgacgtt tacagcgaac
     5941 tttattttgg acgggaaaaa ccgctgcctg cgaaagcgtg ggatcgccac gatggcgttt
     6001 tgcattgctc ttcgttttcg aaatgtctgg tgcctggttt tcgtattggt tgggtcgccg
     6061 ccggaaaaca tgcacgtaaa attcaacgct tgcagttgat gagtacgctt tccaccagct
     6121 caccgatgca gcttgcgctg gtggattacc tttccacgcg ccgatacgac gcccatcttc
     6181 gtcgcctgcg tcgccagctt gcggaacgta aacaacgtgc ctggcaggca ctgctgcgtt
     6241 atctgcctgc ggaagttaaa attcatcata gtgacagcgg ttattttctc tggctggagc
     6301 tccccgagcc gttagatgcc ggcgaattaa gcctggcggc actgacgcat catatcagta
     6361 ttgcgccggg taaaatgttt tctaccggtg aaaactggtc acgttttttt cgttttaata
     6421 ccgcgtggca gtggggagaa cgtgaagaac aggcggtaaa acaattaggc aaacttattc
     6481 aagaacggct gtaatagcgt ttaatttaat tcctcttaga ttgggtaata tgaatttcga
     6541 atagcagtca tatttcctaa ccccttgtct atactccaga agataacctt acagacggca
     6601 taatgcgcgg tagctcacaa cctgattaaa ttttctcagg ggcgaaggtg tgcctgcaag
     6661 ccgccgtcta tggttaaaca aggagatatt tttacggcac ggcggctgaa caattaatta
     6721 cgacaggagt aagaccttat gagcaagaca tttgcccgca gcagcctgtg tgcgctcacc
     6781 atgacaataa tgaccgctca cgccgccgaa ccacctacca atttagataa accggaaggg
     6841 cgactggata ttatcgcctg gccgggatac atcgaacgcg gacaaactga taaacaatac
     6901 gactgggtaa cgcaatttga aaaagagaca ggctgcgcgg tgaatgtgaa aaccgccgcg
     6961 acttccgatg aaatggtcag tctgatgacc aaagggggtt acgatctggt tacggcatcc
     7021 ggcgatgcct cgctgcgttt gattatgggc aaacgcgtgc agccgattaa taccgccttg
     7081 attcccaact ggaaaacgct cgatccgcgc gtggttaaag gcgactggtt taatgttggc
     7141 ggcaaagttt acggcacacc ttaccaatgg gggccgaacc tgctgatgta caacactaaa
     7201 accttcccga cgccgccgga tagctggcaa gtggtttttg ttgagcaaaa tctgccggac
     7261 ggcaagagca ataaaggccg cgttcaggct tatgatggcc ctatctacat tgcggacgct
     7321 gcgttgttcg ttaaagccac tcagccgcag ttgggcatca gcgatccgta tcaactaacc
     7381 gaagaacagt accaggcggt gctgaaagtg ctgcgcgctc aacacagttt gatccatcgc
     7441 tactggcatg acactaccgt gcaaatgagc gatttcaaaa acgagggtgt ggttgcttcc
     7501 agtgcctggc cctatcaggc caacgccctg aaagccgaag gccagcctgt tgctaccgtt
     7561 ttcccgaagg agggtgttac cggttgggct gataccacca tgctgcatag cgaagcgaaa
     7621 catccggttt gcgcctacaa atggatgaac tggtcattaa cgccaaaagt gcagggcgat
     7681 gtggcggcct ggtttggctc gttaccggta gtgccggaag ggtgtaaagc cagtccgtta
     7741 ttaggcgaaa aaggttgtga aaccaacggt tttaactatt tcgacaaaat cgccttctgg
     7801 aaaacgccta tagcagaagg gggcaagttt gttccctaca gtcgctggac gcaggattac
     7861 attgccatta tgggcggtcg ctaacttcgc tggggtgctt tatgacgtac gcagtggagt
     7921 ttgacaacgt ctcgcggttg tacggtgacg tgcgcgcagt agatggcgtc agtattgcga
     7981 taaaagatgg tgagttcttc tctatgctgg ggccgtccgg ctccggcaaa accacctgcc
     8041 tgcgcctgat tgctggcttc gaacagcttt ccggcggggc tatctctatc tttggtaaac
     8101 ccgccagcaa tctgccaccg tgggagcggg acgtgaatac tgtctttcag gactacgcgc
     8161 tatttccgca tatgtcgatt cttgacaatg tcgcctatgg gctgatggtc aaaggcgtga
     8221 ataaaaagca gcggcacgca atggcgcaag aggcgctgga gaaagtggcg ttggggtttg
     8281 tacatcaacg taaaccgtca caactttctg gtggtcagcg ccagcgggtt gctatcgcca
     8341 gagcattggt gaatgaaccg cgcgtattgc tgttggatga accgctcggc gcactggatc
     8401 tcaaattgcg tgagcagatg cagctggaac tgaaaaaact gcaacagtct ctcggtatca
     8461 cttttatctt cgttacccac gatcagggcg aagcgttatc gatgtccgat cgtgtggcgg
     8521 ttttcaataa tggacgcatt gagcaggtcg attccccgcg cgatctctat atgcgcccgc
     8581 gcacgccgtt tgttgccgga ttcgtcggga catccaacgt ttttgatgga ctgatggcag
     8641 agaaactttg tggcatgacg ggaagcttcg ccctgcgacc ggaacatatc cgcctcaaca
     8701 cccctggtga actgcaggcc aatggcacga tccaggcggt gcaatatcag ggcgcggcaa
     8761 ctcgttttga actgaaattg aacggcggtg aaaaactgct tgtgagtcag gccaatatga
     8821 caggcgaaga actgcctgcc acgctcacgc ccggacaaca ggtgatggtt tcctggtcgc
     8881 gtgatgtgat ggtgccgctg gttgaggaga ggtgaatggc gatgaatgta ttgcaatcac
     8941 cttcacgtcc aggtctgggt aaggtgtccg gtttcttctg gcgtaatccg gggctggggc
     9001 tgtttttact gctgcttggc ccgctaatgt ggtttggcat tgtctatttc ggctcgctgc
     9061 tgacactgtt atggcaggga ttttatactt ttgacgattt caccatgtcg gtaacgccgg
     9121 aactgacgct ggcgaatatc cgtgcgctgt ttaatccggc gaattacgac atcattctcc
     9181 gcacgctgac tatggctgtg gcagtcacta tcgccagcgc cattctggct tttccaatgg
     9241 cgtggtatat ggcgcgctat accagcggga aaatgaaagc ttttttttat attgcggtaa
     9301 tgttgccgat gtgggcgagc tacattgtta aagcctatgc ctggacgtta ttgctggcaa
     9361 aagatggcgt ggctcagtgg tttttacaac atcttgggct ggaaccactg ctgactgcgt
     9421 tccttacatt acctgcggtg ggcggaaata cgctgtcaac ttccgggctg gggcgctttc
     9481 tggtgtttct ctatatctgg ttgccgttca tgatcctgcc cgttcaggcg gcgcttgagc
     9541 gtttgccgcc gtcattgttg caggcgtcgg ctgatctcgg cgcacgtcca cgacaaacct
     9601 ttcgctatgt ggtgctgccg ctggcaatcc cgggtattgc cgctggctct atctttacct
     9661 tctcactcac actgggcgat tttatcgtcc cgcagctggt tggtcctcca ggatatttta
     9721 tcggcaatat ggtttattcc cagcaggggg cgattggcaa tatgccgatg gcggcggcat
     9781 tcaccctggt gccgattgtt ctcatcgcac tgtacctggc gttcgtgaaa cgtctgggag
     9841 cgttcgatgc actctgaacg cgcaccgttt ttcctcaaac tggcggcctg gggcggcgtg
     9901 gttttcctac attttcccat cctgattatc gccgcttatg cgtttaacac tgaagatgcg
     9961 gcgtttagtt ttccaccgca gggcctgacg ctgcgctggt ttagcgtggc agcacagcgt
    10021 agtgatattc ttgatgccgt gacactgtca cttaaagtgg cggcgctggc gacattaatg
    10081 gcgctggtgt tagggacgct ggcagctgcc gcgctgtggc gacgagactt tttcggcaaa
    10141 aacgccattt cgctgttact gctgctgccc attgcgctgc cgggcattgt cactggtctg
    10201 gcgctattaa ccgcttttaa aaccatcaat ctggagccgg gatttttcac catcgtggtc
    10261 ggtcatgcga ctttttgcgt agtggtggtg tttaacaatg tcatcgcccg ttttcgccgc
    10321 acctcgtgga gtctggttga ggcgtcaatg gatcttgggg ccaatggctg gcaaaccttc
    10381 cgctacgtag tgttgccgaa tctcagttcg gcgctactgg caggaggaat gctggcgttt
    10441 gccttgtcgt tcgatgaaat catcgttacg acttttacgg caggtcatga acgaacgtta
    10501 ccgttgtggt tgctcaatca gcttgggcga ccgcgtgatg taccggtaac taacgtggtg
    10561 gcactgctgg ttatgttggt aacaaccttg ccgatcctgg gggcctggtg gctaacccgc
    10621 gaaggcgaca atggtcaata accactgata caggaatatg ctatgcaaca taagttactg
    10681 attaacggag aactggttag cggcgaaggg gaaaaacagc ctgtctataa tccggcaacg
    10741 ggggaggttt tactggagat tgccgaggca tccgcagagc aggtcgatgc tgctgtgcgc
    10801 gcggcagatg cagcatttgc cgaatggggg caaaccacgc cgaaagtgcg tgcggaatgt
    10861 ctgctgaaac tggctgatgt tatcgaagaa aatggtcagg tttttgccga actggagtcc
    10921 cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg aaatcccggc gattgtcgat
    10981 gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg gtctggcggc aggtgaatat
    11041 cttgaaggtc atacttcgat gatccgtcgc gatccgttgg gggtcgtggc ttctatcgca
    11101 ccgtggaatt atccgctgat gatggcagcg tggaaactgg ctccggcgct ggcggcaggg
    11161 aactgcgtag tgcttaaacc atcagaaatt accccgctga ccgcgttgaa gttggcagag
    11221 ctggcgaaag atatcttccc ggcaggcgtg attaacgtac tgtttggcag aggcaaaacg
    11281 gtgggtgatc cgctgaccgg tcatcccaaa gtgcggatgg tgtcgctgac gggctctatc
    11341 gccaccggcg agcacatcat cagccatacc gcgtcgtcca ttaagcgtac tcatatggaa
    11401 cttggtggca aagcgccagt gattgttttt gatgatgcgg atattgaagc agtggtcgaa
    11461 ggtgtacgta catttggcta ttacaatgct ggacaggatt gtactgcggc ttgtcggatc
    11521 tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac tgggtgctgc ggtggcaacg
    11581 ttaaaatctg gtgcgccgga tgacgagtct acggagcttg gacctttaag ctcgctggcg
    11641 catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag cgacagggca catcaaagtg
    11701 atcactggcg gtgaaaagcg caagggtaat ggctattact atgcgccgac gctgctggct
    11761 ggcgcattac aggacgatgc catcgtgcaa aaagaggtat ttggtccagt agtgagtgtt
    11821 acgcccttcg acaacgaaga acaggtggtg aactgggcga atgacagcca gtacggactt
    11881 gcatcttcgg tatggacgaa agatgtgggc agggcgcatc gcgtcagcgc acggctgcaa
    11941 tatggttgta cctgggtcaa tacccatttc atgctggtaa gtgaaatgcc gcacggtggg
    12001 cagaaacttt ctggttacgg caaggatatg tcactttatg ggctggagga ttacaccgtc
    12061 gtccgccacg tcatggttaa acattaagtt agatgttagt ggctgttata ggacggccac
    12121 tatcgtttta ggttctgagt ttaatggcgg ttaagtatta gaatgattac attaataact
    12181 agcgtaaaaa ttaggctaaa tgtttgatgg cgatttttca tcctgtcaca gttgattagc
    12241 ataaggcttt agagtatcgt tattaaacta ataatttata agtagaagta ttaacaatga
    12301 aagttcaatt gtacaaattc acggaagata aaaataaaac ccttaccttc agatggacga
    12361 aaaaacactt tgagttttgt atggataata aaatcttttt aaatcataag gggaagaagt
    12421 cgtataaaga aaggaattta tttcttttct caaaaggtga caagataaca attgaagata
    12481 atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa tttctccagt gtcggcgctt
    12541 tttcatttcc gacctgccat ttttcaggga atatacgtat aggtcgattt tgtagtattg
    12601 catcaaatgt aaaaataatg ggtggaaatc atcctttgaa tcgttttact actcacatga
    12661 tgacttataa cggtgaattt gataagtttg ctatgagtga atttgaacgt agctggacat
    12721 tgaaaccatt cattaccaaa cctgaaaatc cgattatagg aaatgatgtc tggataggaa
    12781 atgatgttgt attgaaaggt ggcatcgcaa taggtgatgg tgctgttatt gctgccaatt
    12841 ccgttgttac caaagatgtg cccccatatg ccatagttgc aggagttcct gcaaaaataa
    12901 tcagatttag gtttgattca aatgttattg atgaactgtt gagaataaaa tggtggaatt
    12961 ataactactc agatcttccg gataataaca agtgtgatga tataaattat tttgttgaag
    13021 agatgaatcg attgatatca gatggaaata ttcaagagag ggactataaa aaattcaatc
    13081 tttctgaggt attcagaggc ctataattat ctgttttgca tgataaatgg gcaagataac
    13141 cagggcgggg tttgcaattt caatggggca gcgtcatcaa ggaggttgca aacatcctgc
    13201 catgcatata ctttacgcca tctttttata tttacgttat tccttgaaag tataaagcat
    13261 ttatagactc ccgcgcgtat aaagtattca tgatagaaaa atgtgaaaac aagattccgt
    13321 ttgaatcagt aagctgtttg tatagacgac agtaggggga tgttttctac tcatttcaat
    13381 gtagatgcca gcaaaaagaa gtacatatta aggataatgt tgtaaacctg gagagtatga
    13441 gaagatcttg cgtactgtca gagagttgat ttctctgaca gtacgtgctg cgtgcaaaat
    13501 cactacatca aataaaccag ccaaatctca tagagataag agtaaacaaa ccaacagcag
    13561 caaagacatt tatcagtact acagttctac tggaaacatt catttttgcc accttttatc
    13621 attgccccta tgtgtatagt acgggttaag aaaatccgca ataatactca ttctgaagat
    13681 ggataagggc aagttgctgt ttgatgattt tttctcaaac tggtgaaaaa acttgatgca
    13741 cgtcaaaaaa tgacgcatat ttgcgcgcgt tttattcatc tggctggacg cccgtacatg
    13801 tctctctatc aacatatgct tgttttttat gcggttatgg cagcaatcgc atttcttatc
    13861 acctggtttc tttctcacga taagaaacgc atccgtttct taagcgcctt tctggtgggg
    13921 gcaacatggc cgatgagttt cccggtggcg ctgttgtttt cactgtttta aatcttactc
    13981 actggcttga cggcaccacg cagacttata tcatttggat gaatcgataa atttcacaag
    14041 tggttaagga gaaagtatgt cgcatctgga tgaagtcatc gcgcgcgtgg atgccgcgat
    14101 tgaagagagc gtcattgccc atatgaacga attattaatc gccctgagcg atgacgcgga
    14161 gttaagtcgg gaagatcgct acacccagca gcagcgtttg cgcaccgcta ttgctcatca
    14221 cggtcgcaag cataaagaag atatggaagc gcgccacgaa cagttaacca aaggcggcac
    14281 catcctctga ttaaaatgag cgtctggcta ccaaccaggc gccaaccaca agtaaaattg
    14341 cgccacaaat tgggccgaac aacgcccgta gtggtacgcc atgactgcgg aagatatcca
    14401 gcatcagacc gccaattagc tggctggcga ccagtactgc aatagttgtc gccgctccca
    14461 cattctgata tccgctgata ctggcgaaga caaaaaacga tcccagcaag ccgggtataa
    14521 gtgtccacca gcgcacgctg gacaccagtt cgccaaaccc cgccatgcct tgtttaaacc
    14581 ataaaataga aacaaagagc acaatcccca ccagtgagtt cagcaacatg gcgatgagaa
    14641 tggtagagga ggtctgggtg atgcgcacca tcagcgtgtt ctggactacc agaccaattc
    14701 cggcggcaat caaaaaagca agggtgagcg actgattcat ccaatcgcgt ccggttcagt
    14761 gcgttcgtcg agttgcaact gcataaatgt cagatccagc cagcgcccaa atttagtgcc
    14821 cacctgcggc atttgtgcgg tgacgacaaa tcccagcgac tggtgcagat gcagcgaggc
    14881 ctgattttgc gattcgatcc cggcgaccat gacatgcttc ccgcaatccc gcgcttcatc
    14941 aatcaatcgg cttaacaatt tacgccccag acctttgccc tgatgatcgg gatggacata
    15001 aaccgaatgt tccacggtat ggcgaaaacc atcgaaacta cgccagtcgc caaacgaggc
    15061 atatcccgtc actacgccat cttcttcact caccagcacc ggataacctg ctatggtccg
    15121 cgcttcaaac caggcaatgc ggttatctgc atcaaccgtt tggtcattcc agatagccgc
    15181 cgtatacaac acggcgtggt tataaatttc cgcaatggca gcacaatcgg ctttgcgggc
    15241 aaaacggatg gacatgtctg gcctcgataa aataatgatg atgatgttat caatactatc
    15301 gaaaacagcc cgaagcaacg gattcctcga tgaaattatt gcgtgttgca gaggggatgt
    15361 gacggctgca aacaaaacca atcccgtgag cctgctacta ttgatggatt catgccaaaa
    15421 gcacaggaga gcattatggg gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg
    15481 cgtccacatg gcgcaccggt tccggagaat ttccgtcttg aagaagatga tgtcgccaca
    15541 ccgggtgaag gacaggtgtt actgcgcaca gtttatttgt ccctggaccc gtatatgcgt
    15601 ggacgtatga gcgatgagcc atcttattca ccgcctgttg atattggcgg cgtgatggtc
    15661 ggcggcacgg tgagccgtgt tgtggagtcg aatcatcctg attatcagcc tggcgactgg
    15721 gtgctgggct acagtggatg gcaggactat gacatatcca gtggtgatga tctggtgaaa
    15781 cttggcgatc atccgcaaaa tccatcgtgg tcgctgggtg tgctggggat gccaggcttt
    15841 accgcttata tggggctgct ggatatcggt cagcctaaag agggcgaaac gttggtggta
    15901 gctgcggcga caggtcctgt gggagcaacg gtggggcaaa tcggcaaact taaaggttgc
    15961 agggtggtgg gggttgccgg tggcgcggaa aaatgccgcc atgctatcga ggtgctgggc
    16021 ttcgatgttt gtcttgatca ccacgcggat gattttgccg aacaactggt gaaagcgtgc
    16081 ccaaaaggta ttgatatcta ttatgaaaac gtgggcggta aggtatttga tgcagtgcta
    16141 ccattactta atacatctgc gcgcattccc gtttgtggct tagtgagcag ctataacgct
    16201 acagagctac cacccggtcc ggatcgttta cccctgttga tggcaacggt gctgaaaaaa
    16261 cgtattcgct tgcaagggtt tattatcgct caggattatg gtcaccgcat ccatgagttt
    16321 cagaaggaga tggggcaatg ggtgaaagag gataaaatcc actaccacga agatattact
    16381 gatggtttag agaatgcgcc acagacgttt atcggcctgc tgaagggtaa aaacttcggc
    16441 aaagtggtga tccgcgtcgc gggtgatgat taaagtttca acggcggcgt aagccgccat
    16501 aaaaatccag gcaaaaagct tattttcagc tttaattaac taaccacgtc attgttctgc
    16561 tgctaataac aacaaaggtg ggtggtttat gattattcac tttaatacac caggtgaact
    16621 ccttctgccg tgcaggcagg gttggacaga aaacacgcga aggaattaaa atgcctggaa
    16681 tgggaaaaat gaaacatgtc agtttgactc tgcaggttga gaacgacctg aaacatcagc
    16741 ttagtattgg cgcattaaaa cctggcgcac gcctgattac taaaaatctg gcggagcaat
    16801 taggtatgag tattacacct gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc
    16861 tttctgtcgc acctgcacaa gcatttacag ttccggaagt gaggaaacgt caattagacg
    16921 aaatcaatcg gatccgctac gaactggaat taatggcagt tgctctggct gttgaaaacc
    16981 tcaccccgca agacctggcg gaactccagg aattgctcga gaaattacag caggcgcaag
    17041 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
    17101 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
    17161 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
    17221 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
    17281 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
    17341 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
    17401 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
    17461 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
    17521 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
    17581 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
    17641 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
    17701 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
    17761 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
    17821 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
    17881 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
    17941 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
    18001 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
    18061 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
    18121 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
    18181 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
    18241 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
    18301 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
    18361 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
    18421 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
    18481 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttggggg gccatcggga
    18541 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
    18601 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
    18661 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
    18721 gcccacctgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
    18781 cttcatcaat gcgtacgccc agtttggcat tagctaaatt tttctgtgcg ccactatggt
    18841 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
    18901 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
    18961 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
    19021 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
    19081 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
    19141 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
    19201 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
    19261 gcacgccggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
    19321 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggtac
    19381 tgacaatcat agtctgttca tcagcggcaa aaacaacggg ggaaaggaca agcagtgcgg
    19441 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
    19501 aacgccgtta tggtaatgaa attgtaaatt tatggaaaac gaaacggcac gatacgttaa
    19561 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
    19621 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
    19681 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
    19741 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
    19801 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
    19861 gccagcaaga aaacgcactg tggctcgcca cttcgcaaag ccgcaaactg gataaaggtg
    19921 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
    19981 tcaagccgtt tggtgccacc atcaataaca cgactcagac gttgtggttt ggtaacaccg
    20041 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
    20101 tggatgatcg taagcgcacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
    20161 atgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
    20221 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
    20281 gtctggcgct ggatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
    20341 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
    20401 aagagcactt ctttatcaac attagccttg ataccgccag gcagcgtgca tttatcaccg
    20461 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
    20521 ttgcggcacc agaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
    20581 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtggtgaaaa
    20641 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
    20701 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagccg gacgatgtga
    20761 ttcgtattgc gctgtaatag atgtggcgag ggtgaaaacg gagagacttg cctgactttt
    20821 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
    20881 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
    20941 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
    21001 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
    21061 taatcgaacg ccatcagcac caggacactc agtaaaaaca gtaatgtcag ccaggaagtg
    21121 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttgttt aattgcttta
    21181 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
    21241 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
    21301 acatacacaa ctagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
    21361 gccataaaac tcggtgcgga accgcccatc gccattgagc gcagaatacg tccggtgcag
    21421 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
    21481 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
    21541 tacgcgctcc acggcaatag cataaccagc aacaccacgg agccgacgta aaacaggcca
    21601 atacgccaaa tcacgctgtt aatcgcttag gcaccatggt ctgcggatct ttacattctc
    21661 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
    21721 ccagagcagg cagcaaaccg tgggggaaga agccgccatt atcggtaatt aaatgaaagc
    21781 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
    21841 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
    21901 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
    21961 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
    22021 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
    22081 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
    22141 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
    22201 ccaatgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
    22261 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
    22321 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
    22381 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
    22441 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
    22501 cggcgcagca tcctacccgt tatcgataaa cgatgcaaaa catcccctta caatcctgaa
    22561 ggggaataat acaactgacg aaaaaatgac aaatcttttt gctggttaac ctgtgtactg
    22621 tcctacactt aatctttaaa agattgtgag ggcatatgat taaggtgtat ggcgtacccg
    22681 gatggggctc tacaatcagt gagctgatgc taacactggc tgatattcct tatcagttcg
    22741 ttgatgtcag tggttttgac cacgagggag cttcacgcga gttattgaaa accctgaacc
    22801 cgttgtgtca ggttccgaca ctggcgctgg aaaatgacga gattatgacg gagacagcgg
    22861 cgattgcatt tatggttctc gatcgtcgcc cggaccttgc gccaccggtt gggcgcgccg
    22921 aacgtcagca gtttcaacgg ctattggtct ggctggttgc taatgtctat ccaacattca
    22981 ctttcgccga ttaccctgag cgttgggcat ctgacgcacc tgaacagtta aagaagaatg
    23041 taattgaata tcgcaaatca ctttatatct ggctgaattc acaattgacc gctgaacctt
    23101 atgcctttgg tgagcaacta acgctggtgg actgttatct ctgcaccatg cgcacatggg
    23161 ggccggggca tgaatggttt caggataatg cgcagaatat cagcgcgatc gctgatgctg
    23221 tatgtcagtt accgaaatta caagaagtat taaaaaggaa tgaaattatt tgagtgggat
    23281 accagaagat tatgttattt attattcagt aact
//
LOCUS       DAZUFB010000069        23263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZUFB010000069 DAZUFB010000000
VERSION     DAZUFB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.069"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            351..446
                     /locus_tag="ACHY3M_003115"
                     /pseudo
     CDS             351..446
                     /locus_tag="ACHY3M_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            470..4984
                     /locus_tag="ACHY3M_003116"
     CDS             470..4984
                     /locus_tag="ACHY3M_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFS4317986.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTMALSKVSGNELLVWTAGKKQGEAKPGSESLWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSFADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQSLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNISVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4985..6634
                     /gene="yfaQ"
                     /locus_tag="ACHY3M_003117"
     CDS             4985..6634
                     /gene="yfaQ"
                     /locus_tag="ACHY3M_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFS4317987.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6639..7415
                     /gene="yfaP"
                     /locus_tag="ACHY3M_003118"
     CDS             6639..7415
                     /gene="yfaP"
                     /locus_tag="ACHY3M_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFS4317988.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7690..10539
                     /gene="rcsC"
                     /locus_tag="ACHY3M_003119"
     CDS             7690..10539
                     /gene="rcsC"
                     /locus_tag="ACHY3M_003119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFS4317989.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10739..11389)
                     /gene="rcsB"
                     /locus_tag="ACHY3M_003120"
     CDS             complement(10739..11389)
                     /gene="rcsB"
                     /locus_tag="ACHY3M_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFS4317990.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11406..14078)
                     /gene="rcsD"
                     /locus_tag="ACHY3M_003121"
     CDS             complement(11406..14078)
                     /gene="rcsD"
                     /locus_tag="ACHY3M_003121"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFS4317991.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETSLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQSRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14817..15908
                     /gene="ompC"
                     /locus_tag="ACHY3M_003122"
     CDS             14817..15908
                     /gene="ompC"
                     /locus_tag="ACHY3M_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS4317992.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            16020..17075
                     /gene="apbE"
                     /locus_tag="ACHY3M_003123"
     CDS             16020..17075
                     /gene="apbE"
                     /locus_tag="ACHY3M_003123"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFS4317993.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            17149..18213
                     /gene="ada"
                     /locus_tag="ACHY3M_003124"
     CDS             17149..18213
                     /gene="ada"
                     /locus_tag="ACHY3M_003124"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFS4317994.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            18213..18863
                     /gene="alkB"
                     /locus_tag="ACHY3M_003125"
     CDS             18213..18863
                     /gene="alkB"
                     /locus_tag="ACHY3M_003125"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFS4317995.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18939..20582
                     /gene="yojI"
                     /locus_tag="ACHY3M_003126"
     CDS             18939..20582
                     /gene="yojI"
                     /locus_tag="ACHY3M_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFS4317996.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            20800..22446
                     /gene="mqo"
                     /locus_tag="ACHY3M_003127"
     CDS             20800..22446
                     /gene="mqo"
                     /locus_tag="ACHY3M_003127"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFS4317997.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQNSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(22595..23104)
                     /gene="eco"
                     /locus_tag="ACHY3M_003128"
     CDS             complement(22595..23104)
                     /gene="eco"
                     /locus_tag="ACHY3M_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFS4317998.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         5653 a         5678 c         6202 g         5730 t
ORIGIN      
        1 tcaaattatt gttggttgta taacttgatt acatttttac tctaagtgtt ataaatattt
       61 tatactgata ttagttttat tatatatatt cttaatgaaa atgagcataa aactaagaca
      121 tataatgctt ggcagcttta tgctcttctg attggctgta tgtttgatga caaaaaatta
      181 gcgcaagaag aaaaaatcac cttgcgctaa tgctctgtct caggtcacta atcacaccta
      241 actcattgat taattgtgac tctaaatgta gtgttttgtc gcgttttgca gtatgttatt
      301 taatctatta tatgaataac ataatgattt ttaatgtatt tttgtaattt actctccttc
      361 tgacatggaa ggacacccga tggataccca acgattccaa tcccaatttc attggcattt
      421 atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc ctgtctcttg tgggaacagg
      481 gcttgctaat gctgatgatt cgcttccttc cagtaactat gcgccgcccg ccgggggaac
      541 attctttttg cttgctgaca gcagttttag cagcagtgaa gaggcgaaag tgcgactgga
      601 agcgccgggg cgtgattatc ggcgctatca gatggaagag tacggcggcg tggacgttcg
      661 cctgtatcgt attcctgacc cgatggcatt tttgcgccag cagaaaaacc tgcatcgcat
      721 tgtggtgcaa ccgcaatatc tgggcgacgg gctgaacaat acgctgacct ggctgtggga
      781 taactggtac ggcaaatctc gccgcgtaat gcagcgtact ttctcttctc agtcacggca
      841 gaatgtgact caggcattac ccgaattaca tctcggcaat gccattatta aaccttcccg
      901 ttatgtacag aacaaccagt tttccccgct gaaaaaatat cccttggtgg aacagttccg
      961 ttatccacta tggcaggcta aaccggtcga gccgcagcaa ggggtaaaac tggaaggcgc
     1021 atccagcaat ttcatctcgc cgcagccggg taacatttat attcctctcg gccaacaaga
     1081 gccgggactg tacctcgtcg aggcgatggt tggtgggtat cgggcgacga cggtggtgtt
     1141 tgtttccgat accatggcgc ttagcaaagt gtcaggcaac gagcttctgg tgtggaccgc
     1201 gggtaaaaaa cagggtgaag cgaagcccgg ctcagagagc ttgtggactg acggtcttgg
     1261 cgtgatgacc cgcggtgtga ccgatgacag cggtaccttg cagttacaac atatatcgcc
     1321 agaacgttca tacattctgg gtaaggatgc tgaaggcggt gtttttgtct ccgagaactt
     1381 cttctacgaa agcgaaatct acaacacccg cttgtatatt tttaccgatc gcccgctata
     1441 tcgcgcaggc gatcgtgtcg atgttaaagt gatgggccgc gagttccacg atccgttgca
     1501 ttcatccccc atcgtcagcg ccccggcgaa gctttcggtg ctggacgcta acggcagtct
     1561 gttgcaaacc gtcaatgtca cgctggatgc gcgcaatggc gggcagggaa gtttccgcct
     1621 gccagaaaat gccgtagccg gaggttatga gttacgtctt gcttaccgca atcaggtcta
     1681 tagcagcagt tttcgcgtgg caaactacat caagccacat ttcgagattg gtttagctct
     1741 cgacaaaaaa gagttcaaaa ctggcgaagc ggtcagcggc aaactgcaac tgctttaccc
     1801 ggatggtgag ccggtaaaag atgcccgcgt gcagttaagt ttgcgcgctc agcaattatc
     1861 aatggtcggt aacgatttgc gttatgccgg acgttttccc gtgtcgctgg aaggcagcga
     1921 aacggtgtcc gacgccagcg gtcatgtggc gttaaatctc cccgccgccg ataaaccgag
     1981 ccgctatttg ttaaccgtct ccgccagtga cggcgcggcg tatcgcgtca ccaccaccaa
     2041 agagatcctc attgaacgcg gcctggcgca ttactcttta agtaccgccg cacaatacag
     2101 taatagcggc gagtcggttg tgttccgtta tgccgcgctg gaatcttcaa aacaggttcc
     2161 tgttacgtat gaatggttgc gtctcgaaga ccgcacgagc catagcggag agctaccgtc
     2221 aggcggcaaa tcctttaccg tcaatttcgc taaacctggc aactacaatc tgacattacg
     2281 cgataaagac ggcttaattc tcgctgggtt aagtcatgcc gtcagcggta agggcagcac
     2341 ggcgcatact ggtacggtag atatcgtggc ggataaaacg ctgtaccagc caggcgaaac
     2401 tgcgaagatg ctgattacct ttccggagcc aattgatgaa gcattattga cgctggaacg
     2461 cgatcgcgtg gaacagcagt cgctgctttc gcatccggca aactggctga cgttacaacg
     2521 tttaaacgat acccagtatg aagcccgggt tccagtgagc aattcctttg cgcctaacat
     2581 cactttttcg gtgctgtata cccgtaatgg tcagtacagt tttcagaacg ccgggatcaa
     2641 agttgccgtt cctcagcttg atatccgggt gaaaacggac aaaacccatt accagcctgg
     2701 tgaactggtc aatgtcgaat taacctcgtc gctgaaaggt aaacctgttt ctgcgcagct
     2761 aacggtaggc gtggtcgatg aaatgatcta cgcgctgcaa ccagaaatcg cgccgaatat
     2821 cggcaaattt ttctatccgc tggggcgtaa caatgtgcgt accagctcca gtctgtcgtt
     2881 tatcagctac gaccaggcgc tctccagcga gccggttgcg cctggcgcaa ctaaccgcag
     2941 cgagcggcga gtaaaaatgc ttgaacgtcc acggcgtgaa gaggtggata ccgcggcatg
     3001 gatgccgtca ctcacaaccg ataaacaagg caaagcgtat ttcacgttcc tgatgcctga
     3061 ttcgttaacc cgctggcgta tcaccgcgcg tgggatgaac ggcgacgggc tggtcgggca
     3121 ggggcgtgct tatctgcgtt cggaaaaaaa tctctacatg aagtggagta tgccaacggt
     3181 gtatcgcgtg ggcgacaaac cggcggcagg actgtttatc ttcagtcagc aggataacga
     3241 accggtggcg ctggtgacta aatttgcagg cgctgagatg cgccagacgc tgacgctgca
     3301 caaaggggcg aattatattt cgctgacgca gaacattcag caatctggct tgttaagtgc
     3361 agaactgcaa caaaatgggc aagtgcagga cagcattagc acaaaactgt cttttgtgga
     3421 caacagctgg cccgttgaac agcagaaaaa tgtcatgctc ggcggtggcg agaacgcgct
     3481 gatgttgccc gagcaggcga gtaatatccg gctacaaagt agtgaaacgc cgcaggagat
     3541 tttccgcaac aatcttgatg cgttagtcga tggaccatgg ggtggcgtga tcaacaccgg
     3601 tagccgtctg atcccgctca gtctcgcctg gcgttcgttt gccgatcatc aaagtgccgc
     3661 cgctaacgac attcgtcaga tgattcagga taaccgtctg cggctgatgc aactggcggg
     3721 gcccggagcg cgctttacct ggtggggtga agatggcaat ggtgacgcct tccttacggc
     3781 atgggcatgg tacgccgact ggcaggccag ccaggcgctc ggcgtaacgc aacaaccgga
     3841 atactggcag catatgctcg acagttatgc cgagcaggcg gataacatgc cgttattgca
     3901 tcgggcgctg gtgctggcgt gggcacagga gatgaatctg ccgtgcaaaa cgttgttgaa
     3961 agggttggat gaagctatcg cccggcgcgg aactaaagat gaagatttct ctgaggaaga
     4021 catccgcgat atcaatgaca gcctgatcct cgatacaccg gaatctccac tggcagatgc
     4081 ggtggcaaac gtcttaacca tgacgttgct gaaaaaagcg cagttgaagt ccacggtgat
     4141 gccacaggtt cagcaatatg cgtgggataa agcggcaaac agcaatcagt cgctggcgca
     4201 cacggttgtg ctgctcaata gcggtggtga cgctacccag acggccgcta ttttaagtgg
     4261 tttgaccgct gagcaatcca ctattgagcg cgcgctggcc atgaactggc tggcgaaata
     4321 tatggcgaca atgcctccag ttgttttgcc tgcgcctgcg ggcgcatggg ctaaacataa
     4381 gttaactgga gggggcgaag actggcgttg ggttggtcag ggcgtgccgg acattctctc
     4441 ttttggtgac gaattatcgc cgcaaaatgt gcaggtccgc tggcgtgagc cggcaaaaat
     4501 ggctcaacaa agtaacattt cggtgaccgt tgaacgccag ttgtatcggc ttatccctgg
     4561 tgaagaagag atgagcttta ttctgcaacc ggtgaccagc aatgagattg acagcgatgc
     4621 gctgtatctc gatgaaatta cgcttaccag cgagcaggat gcagttctgc gctatggtca
     4681 ggtggaagta ccgctcccgc cgggagccga cgttgagcgc acaacatggg gcatttcagt
     4741 caataaaccc aacgccgcga aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat
     4801 gggcgaactg gcctatatgg tgccggtgaa agaactgacg ggaacggtca ctttccgcca
     4861 tttgctgcgc ttctcgcaaa aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc
     4921 ctatgcacct gcgcagcaaa gtgttgcggc agggagtgaa tggaccggga tgcaggtgaa
     4981 ataagtgaac tggcgcagaa tcgtctggct attggcgttg gttactctgc caacgcttgc
     5041 ggaggagacg ccgttacaac tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct
     5101 gtcgtcatca ggcgtgacca aagtctcagc attacctgac tcgctcacca cgccgttagg
     5161 gagtttgtgg aaactgtatg tttacgcctg gctggaggat acccatcaac cggagcagcc
     5221 ctaccagtgt cgcggaaact ccccggaaga ggtttattgt tgtcaggctg gggagagcat
     5281 aacgcgcgat accgcgctgg tacgttcctg tgggctctat tttgcgccgc agcggttgca
     5341 tattggcgca gatgtgtggg ggcaatactg gcaacagcgt caggcacctg cgtggttagc
     5401 gtctcttaca acgctgaagc cagaaacctc ggtaacggtt aaatcgctgc tcgattcatt
     5461 agcgacgtta cctgcgcaaa acaaagctca ggaagtctta ctggatgtgg tgctggacga
     5521 ggcgaaaatc ggtgtcgcat ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg
     5581 gtttgccgat gataaacagg aaatccgcca gggtggtttt gctggctggt taaccgatgg
     5641 tacaccgctg tgggttactg gcagtggcac cagcaaaacg gtactaaccc gttatgcgac
     5701 ggtgcttaat cgggttttgc cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt
     5761 ggagctgttt gcccgctatc cgctgaagaa aatcacagcg gaaaagagta cgacggcagt
     5821 aaaacccggc gtacttaatg ggcgctatcg tgtgacgttt actaatggta accacattac
     5881 gtttgtcagc cacggtgaga cgaccttact gagcgaaaag ggcaaactga aattgcagtc
     5941 gcatctcgat cgtgaagagt acgttgccag agttctggat cgcgaggcga aaagtacgcc
     6001 ccctgaggca gcgaaagcca tgaccgtggc gattcgcact tttttacagc aaaacgccaa
     6061 tcgggaaggc gattgcctga ccatccccga cagcagcgcc acgcagcgtg tttctgcttc
     6121 gccagcgacc acgggggcgc gaacgatgac cgcctggacg caggatctca tctatgcagg
     6181 cgatccggtt cattatcatg gcagtcgagc taccgaaggg acactttcct ggcggcaagc
     6241 cacggcccag gcggggcagg gagagcgtta cgaccagata ctcgcgtttg cttatcccga
     6301 caacagcctt agccgctggg gagcgccacg ctcaacctgc cagttattac ccaaagcgaa
     6361 agcctggctg gcgaaaaaaa tgccgcagtg gcggcgtata ttacaaggcg agacggggta
     6421 caacgaacca gacgtgtttg cggtctgtcg tttagtctca ggtttcccct ataccgatcg
     6481 gcagcagaaa cggctgttta ttcgtaactt ctttacgctt caggatcggc tggatttaac
     6541 ccatgagtat ctgcacctgg cctttgatgg ttatcccacc gggcttgatg agaactatat
     6601 cgaaacgctg acccgacaat tattaatgga ctaatgctat gcgaaagatt tttcttccgt
     6661 tgttactggt ggcactttcg cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg
     6721 acgcaccgct ctctggctgg catccggcag agggcgaaga tgccagtttt tcacaatcga
     6781 tcaattaccc ggcgtcgtcc gtcaacatgg cagatgatca aaatatctca gcgcagatcc
     6841 gcggcaaaat aaagaattat gctgcggcgg gtaaagttca gcagggtcgg ctggtggtca
     6901 acggtgccag tatgccgcag agaattgaat ccgatggttc atttgcacgc ccttatattt
     6961 tcactgaagg cagcaacagc gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa
     7021 tgcagtttta ctcaacgccg ggtacaggaa cgattcgtgc acgtttacgg ctggttctct
     7081 cgtgggatac ggacaatacc gatctcgatc tccatgttgt cacgcccgat ggcgaacacg
     7141 cctggtacgg taacaccgtg ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg
     7201 ggtacggacc cgagattttc gccatgccag caccagttca cgggcgctat caggtgtata
     7261 tcaactacta tggtgggcgc agcgaaacgg aattgacgac cgcccaactg acgctgatca
     7321 ccgatgaagg atcggtcaat gagaaacagg aaacatttat tgtaccgatg cgtaatgctg
     7381 gcgagctgac gctggtgaaa agttttgact ggtgatccat cccttcacac aacctggctc
     7441 tctctttttt cagataagac tgctgggcga tctgccgacg ccaggatata accattaaca
     7501 agcgtcggca gattggtcat ttcttatttt tcgtactgag aactgtccat ctactccggc
     7561 tttcctggtt aaggcaatgg tttctaagcg atacgcatta ttgcaatggt gacagaacat
     7621 tgcggtaaca cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc
     7681 ttcgcccctt tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg
     7741 ttcagagcat tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc
     7801 gttaatgcgt tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat
     7861 caggctcagc gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc
     7921 gaaaatcgct tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg
     7981 gatgtgcctg cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc
     8041 tggcgaggtt ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct
     8101 gcggcttacg atctcaaccg ggtattttta atcggcagtg ataacctctg catggccaat
     8161 ttcggtctgc gtgatatgcc agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc
     8221 atcaataaat atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc
     8281 gaaggtccgc gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac
     8341 cggttgcagg cgcttttggg tgtcgagcag accatccgga tggaaaactt tttcttaccg
     8401 ggtacgttgc cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt
     8461 accggaccag aaagtaaaat taagggcgat cctcgctgga tgcaggaacg ctcctggttt
     8521 ggctatacgg aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta
     8581 agcatcgtgt attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt
     8641 aacgcaattt tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac
     8701 gagcgacgta ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag
     8761 ttcaatcgca agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat
     8821 ggcgtcaata ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag
     8881 gaccgccaac gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg
     8941 accagcaaca ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac
     9001 gtggccattt gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag
     9061 gagatggcac aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc
     9121 agtcatgagc tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc
     9181 aaagagttac cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg
     9241 ttgttgaaaa ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag
     9301 atcgaaccgc gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta
     9361 ccgctggtgg tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg
     9421 gccttaaatg gcgacccgat gcgtttacag caggtcatct ccaacctgct gagtaacgcc
     9481 ataaaattca ccgataccgg ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc
     9541 tctatccgtg ttcgcgatac cggcgtgggc attccggcga aagaagtggt gcgcttgttt
     9601 gatcccttct tccaggtcgg aacgggcgta cagcgtaact tccaggggac cggtctgggt
     9661 ctggcgattt gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa
     9721 ccgggaatgg gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag
     9781 aaaaaaggcg tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg
     9841 ctctgtcagt tcctggaaac cagtttgcag cgcagcggca tcgtcgttac aacatacgaa
     9901 gggcaggaac cgactcccga agatgtgtta atcactgacg aggtagtgag taaaaaatgg
     9961 cagggtagag cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg
    10021 ccaggggagt gggtacacag tgtggctgct ccgcatgagc taccggcatt gttggcgcgt
    10081 atttatttga tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa
    10141 gcggtcagcg ataacgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga
    10201 cgtctgctgg cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc
    10261 gtcgatgcgc ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac
    10321 atgccaaata tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg
    10381 ccggtaatcg gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc
    10441 ggtatggaca gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg
    10501 gtatatgccg agagggtcag gaaatcgcgg gaatcgtagg acggataagg cgtttacgcc
    10561 gcatccggca ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt
    10621 taatgtaatc tgttgaaata atgggaatcg taggccggat aaggcgttta cgccgcatcc
    10681 ggcattcagt gcaaatgcca gataagacac taacgcgtct tatctggcct acaggtgatt
    10741 agtctttatc tgccggactt aacgtcacag aagagagata attcagcagg gcgatatcgt
    10801 tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc
    10861 ggttcagctt tttagcgatc tcggtcacca ggaagccttc cgcaaacagg cgcagaactt
    10921 cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca
    10981 ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg
    11041 gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg
    11101 cactaagaat cgccgggttg ttgttcatgg tcagaacaat aatcgacagg cttgggaaat
    11161 ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga
    11221 gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt
    11281 cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga
    11341 acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta
    11401 ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct
    11461 ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc
    11521 agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag
    11581 tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag
    11641 agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct
    11701 gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg
    11761 ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca
    11821 tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata
    11881 tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct
    11941 aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca
    12001 tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata
    12061 tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt
    12121 ttacgcgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg
    12181 ttttgggtct ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct
    12241 tctccggtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc
    12301 tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa
    12361 atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta
    12421 atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca
    12481 attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcatc cgcaagcatg
    12541 ttcgctaact gtatttcatc aaccagccgc accagcacat ctgcctgatt cgccagttgt
    12601 ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc aggttctttc
    12661 agcgcatcgc caatgttttt cataaaggtc atccggctct gctggttttt ctcatacaga
    12721 cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg
    12781 aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta
    12841 ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga
    12901 ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc
    12961 tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt
    13021 aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag
    13081 aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc
    13141 agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag
    13201 accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag
    13261 tggatactga cgctatccgt cccttctttc tcattatcat tgtttcccgt tgccgtcgcg
    13321 tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggc
    13381 agatcaaaag ccacgaccgt tgccagatgt cctggctggt taaaggtagt acgcaaggta
    13441 aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaagctttc gcgttcatcg
    13501 agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgacggtc
    13561 gattctttaa atccggaggt gagatcttta aggggtaggg ttgagatcag caccagactg
    13621 ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgta
    13681 tccagataag tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag
    13741 attaacgctt ccgttttgcg gcgtggtttc tccagatagt agacatcctg tttcaggctc
    13801 gtctcttgta aaccttcgcc ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc
    13861 tgccaggtca cgtaacgcca ggtatcgacg cgcttttgga tagcgtgggt aatgtcgaca
    13921 atctggtaac ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc
    13981 gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt
    14041 gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa
    14101 cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga
    14161 ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac
    14221 aagcgccatt ttgctgtaca aataacctac aaaaagccca acgaaaataa ttgcaaataa
    14281 acaaagattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta
    14341 tagtttttct gtggtagcaa agaataatga aaagtgtgta aagaagggta aaaaaaaccg
    14401 aatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat
    14461 aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga
    14521 cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg
    14581 cttgatgtta ggtgcttatt tcgccattcc gcaataatct taaaaagttc ccttgcattt
    14641 atattttgaa acatctatag cgataaatga tacatcttaa aagttttagt atcatattcg
    14701 tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa
    14761 tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga
    14821 aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg
    14881 ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcctgc
    14941 actatttctc tgacaacaaa gatgtagatg gcgaccagac ctacatgcgt cttggcttca
    15001 aaggtgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc
    15061 agggcaacag cgctgaaaac gaaaacaact cctggacccg tgtggcattc gcaggtctga
    15121 aattccagga tgtaggttct ttcgactacg gtcgtaacta cggcgttgtt tacgacgtaa
    15181 cttcctggac cgacgtactg ccagaattcg gtggcgacac ttacggttct gacaacttca
    15241 tgcagcagcg tggtaacggt ttcgcgacct accgtaacac tgacttcttc ggtctggttg
    15301 acggcctgaa ctttgctgtt cagtaccagg gcaaaaacgg tagcgtaagc ggcgaaggca
    15361 tgactaacaa cggtcgtgaa gcactgcgtc agaacggcga cggcgttggc ggatctatca
    15421 cttatgatta cgaaggcttc ggtatcggtg ctgcagtttc cagctccaaa cgtactgatg
    15481 atcagaacag cccgctgtac atcggtaacg gcgaccgtgc tgaaacctac actggtggtc
    15541 tgaaatacga cgctaacaac atctacctgg ctgctcagta cacccagacc tacaacgcaa
    15601 ctcgcgtagg ttccctgggt tgggcgaaca aagcacagaa cttcgaagct gttgctcagt
    15661 accagttcga ctttggtctg cgtccgtccc tggcttacct gcagtctaaa ggtaaaaacc
    15721 tgggtcgtgg ctacgacgac gaagatatcc tgaaatatgt tgatgttggt gcgacctact
    15781 acttcaacaa aaacatgtcc acctatgttg actacaaaat caacctgctg gacgacaacc
    15841 agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt ctggtttacc
    15901 agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc attgttttca
    15961 gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt cgcatgagga taatcacgta
    16021 tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt gttggttgcg
    16081 atcaaaaacc acaaaccgcc aaaacccacg ctactgaagt taccgttctt gaaggcaaaa
    16141 ctatgggtac cttctggcgt gccagcatcc cgggcattga cgccaaacgc agtgccgaac
    16201 ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg acctataaaa
    16261 aagattccgc gctgatgcgc tttaacgatt cgcaaagttt atcgccgtgg ccggtaagtg
    16321 aagcgatggc cgatatcgtc accacctcgc tgcgcattgg cgcaaggacc gatggtgcga
    16381 tggatataac cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa caacagccgg
    16441 ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc ttacagcacc
    16501 tgacggtcat taatcagtcg catcagcaat atctgcaaaa agacctaccg gatttatatg
    16561 tcgatctctc caccgtcggt gaaggttatg cggcggatca cctggcacgc ttgatggagc
    16621 aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatga
    16681 acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcgg
    16741 ttcaggctgt ggtggatatc aacggtcatg gtattagcac ctctggcagt taccgtaact
    16801 attacgaact ggacggcaaa cgtctttctc atgttatcga tccgcaaacc gggcgtccca
    16861 tcgaacacaa tctggtatcc gtgacggtga ttgctccgac ggcgctggaa gccgatgcct
    16921 gggatactgg cttgatggta ctcgggccgg agaaagccaa agaggttgtt cgccgggaag
    16981 ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg atgtcaccac
    17041 agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgctg gtttttgcgt
    17101 gatggtgacc gggcagacta aaggctatcc ttaaccaggg agctgattat gaaaaacgcc
    17161 acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc gaatgccgac
    17221 agcgaattcg ttttcgccgt gcgcaccaca ggcatctttt gccgtccgtc ttgccgcgcc
    17281 agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg cctgcgaggc actcgccgcc
    17341 ggatttcgcc cctgcaaacg ctgccaacct gataaagcca atgcccagca acatcggttg
    17401 gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac gctggaagcc
    17461 ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa agcgactacc
    17521 ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg
    17581 gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga cagcagcagt
    17641 tactatcgca aagctgacga aacgctgggc atgacggcta aacaattccg tcacggtggc
    17701 gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg cctggtggca
    17761 gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac actaatcagc
    17821 gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgat gtttcagcaa
    17881 catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac gttaccgctg
    17941 gacattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac gataccttgc
    18001 ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa agcggtacgg
    18061 gccgttgcca gcgcctgtgc cgccaacaag ctggctatca taataccctg tcatcgggtg
    18121 gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa agcgcaactg
    18181 ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc gatgctgaac
    18241 cgtggcaaga gccactggcg gctggagcgg taattttgca ccgttttgct tttaacgctg
    18301 cggagcaact gatccgcgat attaatgacg ttgccagcca gtcgccgttt cgccagatgg
    18361 tcacccccgg gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga
    18421 cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc
    18481 ccgccatgcc acaaagcttt catgatttat gtcaacgtgc ggctacggcg gcgggctatc
    18541 cggatttcca gccagatgct tgtcttatca accgctacgc tcctggcgcg aaactgtcgc
    18601 tgcatcagga taaagacgaa ccggatctgc gcgcgccgat tgtttctgtt tctcttggct
    18661 tacccgcgat ttttcaattt ggtggcctga aacgtaatga tccgctcaaa cgtttgttgt
    18721 tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat cacggcattc
    18781 aaccgctgaa agcggggttt catccactca ccggcgactg ccgctacaac ctgacattcc
    18841 gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgcg cgaagatgtt
    18901 taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt gtacttgtct
    18961 ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc agtgcggcat
    19021 taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg gataccagtc
    19081 tgctggtatt gccggagttt ctgggattat tgctgctgtt gatggcagtc actctcggat
    19141 cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt agtgaattta
    19201 tcaagcggat tctggatacc aacgtcgagc gcattgaaca actcggtagc gcctcgttgc
    19261 tggcgggatt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt cttccggaac
    19321 tggtgcaggg gatcattctc actatcggtt ccgcggcgta tctgtggatg ctgtcgggca
    19381 aaatgttgct ggtaacggct atctggatgg cgatcaccat ctggggtggt tttgtactgg
    19441 tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagacagg ctgtatacgg
    19501 attttcaaac tgtactcgaa gggcgcaaag agctgactct gaaccgggaa cgcgccgagt
    19561 atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccaccat attatccgcg
    19621 cagacacctt ccatcttagc gccgtgaact ggtcaaacat catgatgttg ggcgcaatcg
    19681 gcctggtgtt ctggatggcg aacagtctcg gttgggctga taccaacgtt gccgcgacct
    19741 attcgttgac gcttttattc ctgcgtacgc cgctgctttc ggcggttggc gcattgccga
    19801 cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc gcgcctttca
    19861 aagcagagtt tccgcgcccg caggcgtttc ccaactggca aacgctggag ctgcgtaacg
    19921 tgacgtttgc ttatcaggat aacgcgtttt ccgttggtcc gattaatctc accatcaaac
    19981 gtggcgagct gctgtttctg attggcggca acggtagcgg aaaatcgacg ctggcgatgt
    20041 tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt gctggatggc aaacctgtca
    20101 gcggcgaaca accggaagat tatcgcaaac tgttttcggc agtgtttacc gatgtctggc
    20161 tgtttgatca actgctgggg ccggagggta aacccgctaa cccgcaactg gttgagaagt
    20221 ggctggcgca gctgaaaatg gctcataagc ttgagttaag caacgggcgt attgttaacc
    20281 tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg gcagaagaac
    20341 gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc cgtcgtgagt
    20401 tttatcaggt gttgctgccg ctgatgcagg agatgggtaa aactattttc gccatcagtc
    20461 atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac gggcaactta
    20521 gcgagctgac gggcgaagag cgcgatgccg cttcgcgtga tgccgttgcc cggacggcat
    20581 aatattacga cgctgacctg gcggactgct gccgtcaggt caatatgcta aaaatccctt
    20641 catttctaat aaccctataa ttaatcaacg aaattataat gtttctaaaa ttagaatata
    20701 atttataaac attatttaaa tgttgttact taagtgttac cgttgatgcc gcgcaaatct
    20761 cacctgcaat aaagcgacta aaagtaaggc attaacaaga tgaaaaaagt gactgccatg
    20821 ctcttctcga tggccgtggg gcttaatgcc gtttcgatgg cggcaaaagc gaaagcgtcc
    20881 gaggagcagg aaactgatgt actgttgatt ggcggcggca ttatgagcgc cacgttgggg
    20941 acctatttac gcgagctgga gcctgaatgg tcgatgacca tggtggagcg cctggagggt
    21001 gtcgcgcagg agagttcgaa cggctggaat aacgccggaa ccgggcattc tgcactgatg
    21061 gaactgaact acaccccgca aaacgccgat ggcagcatca gtattgaaaa agcagtcgcc
    21121 attaacgaag catttcagat ttcccgccag ttctgggcgc accaggttga gcgcggcgtg
    21181 ctgcgtactc cgcgttcatt tatcaatacc gttccgcata tgagctttgt ctggggcgag
    21241 gataacgtta atttcctgcg cgcccgttac gccgcgttgc aacaaaactc gctgtttcgc
    21301 ggtatgcgtt actctgaaga tcacgcgcag atcaaagagt gggcaccgtt agtgatggaa
    21361 gggcgcgatc cacaacagaa agtagcggca acacgcacag aaattggcac tgatgtgaac
    21421 tatggcgaga tcacccgcca gttaattgct tccttacaga agaaatctaa cttctcgctg
    21481 caactcagca gcgaagtccg cgccctaaag cgtaatgacg ataacacctg gaccgttacc
    21541 gttgccgatc tgaaaaacgg cactgcacag aacatccgcg ccaaatttgt ctttatcggc
    21601 gcgggcggtg cggcgctgaa gctattacag gaatcgggga ttccggaagc gaaagactac
    21661 gccggtttcc cggtgggcgg acagttcctt gtttcggaaa acccggacgt ggttaatcac
    21721 catctggcga aggtttacgg taaagcatcc gttggcgcac caccgatgtc ggttccgcat
    21781 atcgataccc gcgttctgga cggtaaacgc gtagtgctgt ttgggccatt tgccaccttc
    21841 tcaaccaaat tcctcaaaaa cggttcattg tgggatctga tgagttccac caccacctct
    21901 aacgtgatgc cgatgatgca cgtcgggctg gataatttcg atctggtgaa atatctggtg
    21961 agtcaggtga tgttgagtga agaggatcgt tttgaagcgt tgaaagagta ctatccgcag
    22021 gcaaaaaaag aggactggcg tttgtggcaa gcggggcagc gcgtgcagat tatcaagcgt
    22081 gatgccgaga aaggtggcgt actgcgtctg ggtactgaag tggtcagtga ccagcaaggg
    22141 accattgccg cgctgctggg ggcatcgcca ggggcgtcaa ccgccgcgcc gattatgttg
    22201 gatctgctgg aaaaagtatt tggcgatcgc gtttccagcc cgcaatggca ggctacgttg
    22261 aaagcgatcg ttccgtctta tggacgcaag ctgaacggtg atgtagcggc aacagaacgc
    22321 gagttgcagt acaccagcga agtgctgggg ctgaaatacg acaagccgca ggcagcagat
    22381 agtacgccaa aaccgcagtt gaaaccgcaa cccgttcaaa aagaagtggc ggatattgcg
    22441 ttgtaatgat gcgccacatc cggcatggta tgccggatgt ggcgtatgct gataagacgc
    22501 gccagcgtcg catcaggcac cggctcgggc gttagatgtc ggatgcggcg tgaccgcctt
    22561 attcggccta cggggtgccg cacttcacag cattttagcg aactaccgcg ttgtcaattt
    22621 tctcttccgc cttccagacg cggtacttca catctacatt gtctggcgta tacaccacga
    22681 tcggcagctt gctgttgtaa cgcagcattc cagcatcgcc cagatacgcg gtgacaaatt
    22741 tcttctcttt cttgccatcc gggcaggcca tcatcgtgga aaccggcgag ctaactttat
    22801 caaagacata ataatcatag ccccagcctt ccagcgtttt gctttccagc ttcccgccga
    22861 gacgatgcaa attgcaatcg acttccagcg tctgaccgat taacagttct actttcaggg
    22921 tagattcatc ttcttgcggg gttaactgaa tcacctgacg cttcatccct ttttcagctt
    22981 gtggataagg cgcgattttt tccagtggct ggacgctttc tgccgcccag gcggaagtgg
    23041 tagcgaaagc ggcaaacaat actgcaggta gaatggtctt catttttttg ctttccttct
    23101 tcaatttata ggttttcacc gatgatgtcg ctgaaggtta acatattcat cagatttatt
    23161 aatttgcttt atgaataact taaagaacat tcagacaatt ttacctgctc ttttagtgtt
    23221 aaattctgat gagggagcaa accagcagaa atatctctgg tga
//
LOCUS       DAZUFB010000070        23061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZUFB010000070 DAZUFB010000000
VERSION     DAZUFB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.070"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            443..1513
                     /locus_tag="ACHY3M_003129"
     CDS             443..1513
                     /locus_tag="ACHY3M_003129"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4317999.1"
                     /translation="MLQYTSVEICAGAGGQALGLHNAGFTHRALVEIDPAACETLRLN
                     NELHSLGWENIIEGCVKHFAEHTAYNFSDIDLVAGGVPCPPFSKAGKQLGKDDERDLF
                     PTALKIVNIIKPKAVMIENVSGLLDPKFKQYRQELDHAFSAMGYKTFWKLHNASDYGV
                     PQLRPRVLLVALRGKYSAHFRWPSETLIPPTVGDALFDLMSQGGWEGAEAWRKAANNI
                     APTLVGGSHKHGGPDLGPTRAKKAWQALGVNGHSIADSSPEKGFTGYIGRDGNIRAGL
                     ENMPRLTVRMAARIQGFPDWWEFSGKKTAAYRQVGNAFPPPVAEATGRQIRKALEAVD
                     LMKKKQLASPLESNDVQSLRIA"
     gene            complement(1492..2151)
                     /locus_tag="ACHY3M_003130"
     CDS             complement(1492..2151)
                     /locus_tag="ACHY3M_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4318000.1"
                     /translation="MELFENYLSALPKKKRLSKVKLIILKRLWVSDDFDFPKPWVPST
                     ELLELTGQKYFDRRTRELRDELGCDLESSYIPEFSGHAWRLKSSMIAPPMEREYLTES
                     QRSSLFSNCSNTCATCGKTVAAGVRGLQADHKIPLSRGGGNELTNWQALCHNCNVGKR
                     RACEGCTEDCYTCSWAFPEKFGITTMLHLPEASLRRAQQLADAKGFSLDEIVDYAIRK
                     L"
     gene            2697..3056
                     /locus_tag="ACHY3M_003131"
     CDS             2697..3056
                     /locus_tag="ACHY3M_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318001.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGDTNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(3049..3591)
                     /locus_tag="ACHY3M_003132"
     CDS             complement(3049..3591)
                     /locus_tag="ACHY3M_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFS4318002.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
     gene            complement(3712..3897)
                     /locus_tag="ACHY3M_003133"
     CDS             complement(3712..3897)
                     /locus_tag="ACHY3M_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318003.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            complement(4193..4474)
                     /locus_tag="ACHY3M_003134"
     CDS             complement(4193..4474)
                     /locus_tag="ACHY3M_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4318004.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            complement(4706..4897)
                     /locus_tag="ACHY3M_003135"
     CDS             complement(4706..4897)
                     /locus_tag="ACHY3M_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318005.1"
                     /translation="MTPREIELLTIAKLEHGGHQLSPAELRELRRQLAEGPVIARRYR
                     EMMTSPAYRWSKPAPLRAR"
     gene            4911..5222
                     /locus_tag="ACHY3M_003136"
     CDS             4911..5222
                     /locus_tag="ACHY3M_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318006.1"
                     /translation="MVIGIILLARVILHWPEPLCVKSMRELHMLCVKLQLKMSCSGGN
                     FLVNSVLVDTNMDTSLLDTTQSQIVTMLNLKQRSLYAPYQIKRSHAQNGLQENGNLLM
                     G"
     gene            5176..5334
                     /locus_tag="ACHY3M_003137"
     CDS             5176..5334
                     /locus_tag="ACHY3M_003137"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4318007.1"
                     /translation="MLRTGFKKTAIYSWVKTGTFPQPVKIGRSARWSLEEVEAWIQNK
                     LDSRAGNQ"
     gene            6329..6718
                     /locus_tag="ACHY3M_003138"
     CDS             6329..6718
                     /locus_tag="ACHY3M_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318008.1"
                     /translation="MIAILNKAKGRVGVNAQAVIMVFLGLIVAKLYPDWWKGALATVA
                     IYLLLSVVKRSFVKTVNQAKRFSFKNLINYITGLIFCLSILIASAFLPVSIANFFGAP
                     YEVGYLFSVFCLVLSLLYLWMRAKNKN"
     gene            6789..7016
                     /gene="ykgL"
                     /locus_tag="ACHY3M_003139"
     CDS             6789..7016
                     /gene="ykgL"
                     /locus_tag="ACHY3M_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFS4318009.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(7051..7191)
                     /gene="ykgO"
                     /locus_tag="ACHY3M_003140"
     CDS             complement(7051..7191)
                     /gene="ykgO"
                     /locus_tag="ACHY3M_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFS4318010.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(7191..7454)
                     /gene="ykgM"
                     /locus_tag="ACHY3M_003141"
     CDS             complement(7191..7454)
                     /gene="ykgM"
                     /locus_tag="ACHY3M_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4318011.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(7818..7919)
                     /gene="ykgR"
                     /locus_tag="ACHY3M_003142"
     CDS             complement(7818..7919)
                     /gene="ykgR"
                     /locus_tag="ACHY3M_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFS4318012.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(8393..8482)
                     /locus_tag="ACHY3M_003143"
                     /pseudo
     CDS             complement(8393..8482)
                     /locus_tag="ACHY3M_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9034..13282
                     /gene="fdeC"
                     /locus_tag="ACHY3M_003144"
                     /pseudo
     CDS             9034..13282
                     /gene="fdeC"
                     /locus_tag="ACHY3M_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
     gene            complement(13403..14260)
                     /locus_tag="ACHY3M_003145"
     CDS             complement(13403..14260)
                     /locus_tag="ACHY3M_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4318013.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            14509..15378
                     /locus_tag="ACHY3M_003146"
     CDS             14509..15378
                     /locus_tag="ACHY3M_003146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4318014.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQREVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(15538..16131)
                     /gene="rclC"
                     /locus_tag="ACHY3M_003147"
     CDS             complement(15538..16131)
                     /gene="rclC"
                     /locus_tag="ACHY3M_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFS4318015.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIFFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQPSNESSSTLKTEY"
     gene            complement(16143..16379)
                     /gene="rclB"
                     /locus_tag="ACHY3M_003148"
     CDS             complement(16143..16379)
                     /gene="rclB"
                     /locus_tag="ACHY3M_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFS4318016.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(16488..17813)
                     /gene="rclA"
                     /locus_tag="ACHY3M_003149"
     CDS             complement(16488..17813)
                     /gene="rclA"
                     /locus_tag="ACHY3M_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFS4318017.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            18039..18893
                     /gene="rclR"
                     /locus_tag="ACHY3M_003150"
     CDS             18039..18893
                     /gene="rclR"
                     /locus_tag="ACHY3M_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFS4318018.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSVRYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            19420..20139
                     /gene="ykgE"
                     /locus_tag="ACHY3M_003151"
     CDS             19420..20139
                     /gene="ykgE"
                     /locus_tag="ACHY3M_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFS4318019.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            20150..21577
                     /gene="ykgF"
                     /locus_tag="ACHY3M_003152"
     CDS             20150..21577
                     /gene="ykgF"
                     /locus_tag="ACHY3M_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFS4318020.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKNFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            21570..22265
                     /gene="ykgG"
                     /locus_tag="ACHY3M_003153"
     CDS             21570..22265
                     /gene="ykgG"
                     /locus_tag="ACHY3M_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFS4318021.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(22511..>23061)
                     /gene="ykgH"
                     /locus_tag="ACHY3M_003154"
     CDS             complement(22511..>23061)
                     /gene="ykgH"
                     /locus_tag="ACHY3M_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFS4318022.1"
                     /translation="LYINKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVI
                     QRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLS
                     IVISVMFCLVKKMVDEYQQNSG"
BASE COUNT         6202 a         5112 c         5541 g         6206 t
ORIGIN      
        1 gtagatatct tacgagcacg acaaacaacc gacatgttgc gacgaagcca aagctgaatt
       61 ccacaagcag cgtgccaatc tttcttattc atttgttctc tgcagaatgc gtctgctatt
      121 tacagcggac gcaatgcatc ttacggacaa aaaatcatgt aaacagatag tagttctttt
      181 cgtactcagc taaggcattt tattcagtcc acatgagatt ctgcctgtct gatcaagaga
      241 atgccaatag tactgtcgaa tgttagttat ttgataaata taggattcca aaacaaagtt
      301 gtagattgcc ttgtcattca gcaatcaagt aactaagctg accggttcaa tagcgacggt
      361 actacttgtt ttgtacaatt tgttgcgtaa tggtctctga tcagataaac atacaactgt
      421 ttttatatac aggtttaatg acatgttaca atacacatct gtagagattt gtgcaggggc
      481 aggaggtcaa gctctagggt tacataacgc tggcttcact caccgcgcgc tcgtcgagat
      541 tgatcctgct gcctgcgaaa ctcttcgcct caacaatgag ctccattctt taggttggga
      601 aaatatcata gagggttgcg ttaagcattt tgctgaacat actgcatata atttttctga
      661 tattgacttg gtcgcagggg gagtgccctg cccgccattc tcaaaagcag ggaaacaact
      721 aggcaaagat gatgaacgtg atttgtttcc aacagcgctc aaaattgtga acatcattaa
      781 gccaaaagca gtaatgattg aaaatgtttc aggtttatta gacccaaagt ttaaacaata
      841 ccgacaagaa ctagatcatg ctttctctgc aatgggttat aaaacctttt ggaaacttca
      901 taacgcttct gattacggag ttcctcagct tcgtccccga gttcttttag ttgcattgcg
      961 tggtaagtat tccgctcatt ttcgttggcc gagtgaaacg ttgataccac cgactgtggg
     1021 tgatgctctc tttgacttaa tgtcacaggg tggctgggaa ggagccgagg catggagaaa
     1081 ggccgctaac aatatagccc ctacacttgt tggcggttcc cataagcatg gaggtccaga
     1141 ccttggaccg acgagagcca aaaaagcttg gcaagcgctt ggagtcaacg ggcattccat
     1201 agctgatagt agccctgaaa aggggtttac tgggtatatt ggaagagacg gaaatattcg
     1261 agcaggtctt gaaaatatgc ctcgtttgac ggtgagaatg gctgcacgaa tccaaggttt
     1321 cccggactgg tgggaattct ctgggaaaaa aacagctgct tatcgtcaag taggtaacgc
     1381 ttttcctcct cctgttgccg aagcaacagg aaggcaaatt cgtaaagcac tcgaagcggt
     1441 tgatctaatg aaaaaaaaac agttggcatc accactggaa tcgaatgatg ttcaaagctt
     1501 gcgtattgcg tagtcaacga tttcgtctaa agaaaaaccc tttgcatcag ctagttgttg
     1561 ggctcttctt aaactagctt ctggcagatg gagcattgtt gttatcccaa atttttcagg
     1621 aaatgcccat gagcatgtgt aacaatcttc agtacatccc tcacatgctc ttctcttacc
     1681 tacgttgcaa ttgtggcaaa gagcttgcca attggttaac tcatttccac caccgcgtga
     1741 caaaggtatt ttgtgatccg cttgtagccc cctgacccca gcggcaacag ttttaccaca
     1801 tgttgcacat gtattgctgc agttagaaaa taaactggat cgttgtgatt ctgtcagata
     1861 ttccctttcc attggcgggg caatcatgct tgatttcagc cgccaagcat gtcccgagaa
     1921 ttccggaatg tacgagcttt cgagatcaca gcctagttca tcacgcaact ctcttgttcg
     1981 tctatcgaag tacttttgcc ctgtcaattc cagtaattct gtcgatggga cccaaggttt
     2041 aggaaaatca aaatcatctg aaacccataa tctctttagg ataatcaact tgactttact
     2101 caatcgcttt tttttaggta acgctgacaa ataattttcg aataattcca tactatctct
     2161 cgaaagaaac cttaatctcg caagggatta caccatagat ggaagtagac aggaaagggc
     2221 tatctaaaag ggcaggcata tagagcaata atcaatgaaa caacctgtac aggcctgcta
     2281 agctgccccc ccaaatgtcc aacatcaata tcagaaatat tgtttcatca agatgaaaaa
     2341 atcaacaaga ctgagccaat ctgagatacg gatgctctat tgttgattta tgttgacgaa
     2401 aattacttac acatggagtt acacggaaaa aatcatgatg cagacaaaga gaggatttaa
     2461 tcgtaattgc ttgatataaa tggtgcgata ataggaacaa tacacatagg cgataagctt
     2521 tgtttttgtt gcctttttat tgttcatttt ttatagatac ccccaaaagt acccccatat
     2581 ctttttccgc acctgaaatt tggttgttat tcatacagca tatcaggcgg taacgctctc
     2641 cctttaaccc acttattgta tgatagtgct atcccacaat aaggaatcag ccagccatga
     2701 gaatgaccag tcgcaaaaaa gagatactta gctactttga accggataac ctcgaatggg
     2761 tgactggtga gattggtgcg ccgccgttcg atgtgtcggg tgtggcctac ctgcttcatg
     2821 gcatggtgtc gttcgataag cgtcaccagc tcgaatcaac ccggcgcaca ctggaaagca
     2881 tggttgctgg tggactgctt gagcgggtga cagtgtatga atcgcgccag atacggcgcg
     2941 gcggtgatac taacgcaacg gtcgtgcgct acggtttacc cggtcagtgc gctgttatgc
     3001 gcgatactgg cggcgctgac aacgcaatat caggtgaata catgcgggtc agttaatagc
     3061 ctgcaatctg tcgttgatgc ggctcaggtc gtaaacccgg cgttcaatat ccagcatcag
     3121 gaaattgtgg atctcggcct gctcaggatt atcagactct ctggcctgta atagcaattc
     3181 attgtaacca tgcaagccgc ctgagttttc cgccggggtg gtgcctttcc cacgcaggca
     3241 gctaatgggc tgattactcg tatcgtgtgt ttgcttctcc agcacaatga cgcaatccca
     3301 ccagtcgcca aagtcatacg tgtaaaccag cttgtcaccc tcattctgga gcgccgccca
     3361 tagtggcgtg attgccgcgt tacccatgct ttcatcctca ccccatacgc gatactcgct
     3421 acctggatca cctttacgcc ctttctcgaa catatacagg tgttcgtcat tccagccaaa
     3481 agctaactgg atcacggcat gaagtttact aaaggggatt tgctctggaa ccagcacacg
     3541 ccgccatata ggtggtttgc tgtctcggat ggttatcttt agctggtaca tgcaaatcct
     3601 gaataaatgc gaatcggaag gataacgata ctgacgtact cacaattctg taaaggtgaa
     3661 taaaggaaat ttgcttaata cgcataagtt acatgtggaa taatcccata tctacctgcg
     3721 cggcgctggc ttcttccact ggtaggcagg tgctcgcatc atttcgcgga acctgtcgcg
     3781 ccgggctata tcggcattaa ctgatcgttc tatctcccgt tgatctgcct gtgtaagctg
     3841 gtggcctccg tgctcaagtt tggcgatggt caataaggct atttcgcgtg gtgtcatacg
     3901 tcataccctc tggaaggatg aaacacgccc ggattaaatt tcatttttcg cgggtataaa
     3961 aaaacgatgg ggcgggcagt cccggatagt cagggctgta gagatttgac taccccctcc
     4021 ccactggatt caggttactg ctattccgtt aagcgctgct tacggggata gctgcgttgt
     4081 gattttgacc attcaggtcg ccgctatttc ctcaccgatt gggtgctacg cgcgcgtact
     4141 tgtcccgaaa taccagaaac cgccagccag aggatgattt ccctctggtg gtctaccttt
     4201 gcctcttgcc gccatttaac cggaaggcgt taccgttcgg ttgcgctatc cccatgcggt
     4261 gttgcgtttc ggtaacagct ttatgcaggt cgccaaattc ctcaaccttc acgggcgggc
     4321 gcttcacgtt ctccagacac gtttcacggc ggcgctttat ccattcccga tcatcgtcct
     4381 cggtgcataa cagcatgact tccatccatc gtgcggcagc tctccggtat agcccttttg
     4441 actccagctc ttcagctttc ctgtccctta tcatttctgc ctctcagatt atatagataa
     4501 gcaaaaaggt gttggttcag ttggttcagt tggttcaatt tgtaaagagt attgttttta
     4561 aaagaattat tcgcaaaaag tgaaccaaca caccccattt ttgaaccaac aaaaagaggc
     4621 cgtgaaccaa cacgccgttt aaaaaccgct aattatgagg gacatccacc agcacagggc
     4681 ttgtctagtg ctggttaggt gctgctcacc gcgcacggag cggcgctggt ttgctccagc
     4741 gatatgcggg gctggtcatc atttcgcggt accgcctggc tattacaggg ccctctgcca
     4801 gctggcgcct taattcccgc agttctgccg ggctgagctg gtggccgcca tgctccagct
     4861 tggcaatggt cagcagctcg atttctcgtg gtgtcatgga ttcccctctg atggtgatcg
     4921 gcattatatt gctagccagg gtgattcttc actggccaga gccgttgtgc gtgaagtcta
     4981 tgcgtgaact ccatatgcta tgtgttaagt tacagctcaa aatgtcctgt tccggtggta
     5041 actttttggt aaacagcgtt ttggtggata ccaacatgga tacctcgctt ttggatacca
     5101 cccaaagcca gattgtcact atgcttaatc taaagcagag gagcctctat gcgccttatc
     5161 aaattaagcg aagtcatgct cagaacgggc ttcaagaaaa cggcaatcta ctcatgggtt
     5221 aagacgggca cgtttccaca gccagttaaa attggtagat cagctagatg gtcgcttgag
     5281 gaggttgaag cttggatcca gaacaaactt gattcgcggg ctggcaacca atgattagct
     5341 ttaatatcga gtggtggata gtccatggac aagctgatgg gcgcactcga cgaaaacgat
     5401 gcgccaggcg gactgtgtcg ctcataaacc gggagttggc cagaggactt agttgtcctt
     5461 tgcaattctg caggttcgca cgtgaaagta gccaaaaccc tgcgaacctg agtgcaaacc
     5521 tgcaactgcg aaccactgag gtggcctctc atagctggtg gtcatactgg atattaatca
     5581 ggcctgccac aaccataaca atccataaca gacgcatcgc accaacaatt actaataggt
     5641 ccgctcgcaa cactaatttt gactaagcct gaacgcaatg ctcagaaaga acgaagaatg
     5701 gaatgaatcg taccatcatg ttgctccatg atttttacgt tcaagacagt tgcttaatct
     5761 cgcctcgcaa ttcagccccc ttgccgttat cgagaagtct ttctccttac ggttaatgct
     5821 atcggtcttc atattaccag cagcatgcgc catccgatta tttcgatgct acgcgcgcgt
     5881 acttgtcgca aaataccagg caaaggccag attcagaacg tcgtgataac caccagtaca
     5941 acatgagagg tcatgtaacg cgatttatca caccagatac atgtgattgg tgtacccgca
     6001 taaaacatcg tcatagcgcg tcctgtgcgc atatacgccg ataatcaaca catcaccacc
     6061 actctgtctc atgtagagaa cagttttatg gtatcccccc gccatcgtgt gcatactggt
     6121 cggacgtagt tgcaaaattt tttgcaactg atacccctcg cgcgcgtact tgtctcgaaa
     6181 ttggtgtttt gctgtggcag tgatatcaga aaaatcttcc tgtttcacat aaagcggata
     6241 tgcgcgtact gtaacggccc catcaacggg gatcggattt gaaatcccga cccaagctat
     6301 gtaccaaatg ccatgtcttg gatatatgat gattgccatt ctaaacaaag ctaaagggag
     6361 ggttggtgtg aatgctcagg ctgtcatcat ggttttcctt ggtctgattg tcgcaaaact
     6421 atatccagac tggtggaaag gggcattagc aactgtcgcc atctatttgc ttttatccgt
     6481 cgttaagcga tcgttcgtta agaccgtaaa tcaagcaaaa cgtttctcat ttaagaattt
     6541 aataaactat attactgggc ttattttttg cctttcaatt ttgatcgcct cagctttttt
     6601 gcccgtatca atagctaatt tttttggcgc accatatgag gtaggttatc tattctctgt
     6661 tttttgtctt gttctttccc ttctttacct atggatgaga gcaaagaaca aaaattagcc
     6721 atccatgacg tttttatgtt agtaccaaca gatttaccac atttatcatc ttaacagttt
     6781 gagtgaaaat gcaggctgcg ccgcaaaggg aggagactga gtggcgtgtt cagtctaaaa
     6841 gagggttgat gccagcatac aggggcgaag ctggtcaaca agtcaacatt aagataatgg
     6901 aatattcgga gagaaacgtc aggcagttgg cctcaaatga acaggaagaa tatatcccca
     6961 ggaaaattaa tgttggtgtt ataaacacac caacattaat ccgatctgat tattaatcag
     7021 acaaatatcg ttaattcgtc gaattttgaa tcaacgtttt ttcttacgac cctgaacggc
     7081 cttaaaacgt ggattagatt tacaaatcac atacaaccgt ccttttcgct tcacaatctg
     7141 acagtctgga tggcgttctt ttgcggtacg cagagagtta agaactttca tcacgccccc
     7201 tttttcgtgc taacaaaacg accaaaacgt tgggtgaatc gtgcaacatt tccttctgat
     7261 gccactgttc tcagcttccc tgtatagaac gggtgcgatt tagaagagac atcaattgtc
     7321 acgtacggat acgttacgcc atccagctca atctcacggt ctgttttgat agtcgagccg
     7381 attttaaagt actcatcaac actggtgtcg tggaacacca cagtacgata ctcaggatgg
     7441 atattgggct tcatcatttt tacctgttat gttataacat aaccgtaaag atacatgctc
     7501 atccggatca tttcaacacc caatccctgt gcccatttgt ggtatgtgga atttccccaa
     7561 cggggaagtc tggcattgtt agcagccgtt ggcgacgctc ccataaacgc ctggatgagt
     7621 gattgatgat ggttttgctg agagtatgga tttttcgaga atggaggtta tctccacttt
     7681 actggccgat gatttatcgc agtatgattg attacctgac agcgtgtatt cctctcccac
     7741 ggatataata attacttaca aaaagggaga ggatgcatat tttaaatatc actgaagtga
     7801 acagtttatt gccgttatta atagaaatgg agaaataaat aggcgtattc tacaattgcg
     7861 acaaaaacaa cgatattaat cagtttatga ctgatttgtt gtactttatt ctctttcatt
     7921 ggtacttcct cgctttaaaa aagagtgcac ttcgtaagtg cccttatata aataacgagt
     7981 ttggtcaacc aattttttga catgtatcac aaatttgaat agatgtatta catcagctat
     8041 cttttattgc accaacgtca ttgatatatg tcgactgaag tcagttccgg gaatgagtct
     8101 gatctcaaga ctggcccagt ccgggcgttg attggcgctg aggagcatac ctcatttcat
     8161 cataatgtcg tatctcctgg ggtgttatac aagatatcgt tgttggtaac ctgggaaagg
     8221 aattgagttc tattaaaccg tcaactatgc cggatacata ctggattaca ctgcaggcac
     8281 gccttatgag agaacgtgcc gcagtgacgg gttaattatc tgaaagaatt tgtgaggctg
     8341 tatcggttac tcattgattt gatagtttta ctctcgggag aataatagat atttaatcca
     8401 ttaacggaaa ccagccagtt cctttcgatg cctgaatttg atcccatagt ttatccggaa
     8461 tggttaattc atgaacaagt tgtgttatcg ttcatgagaa gcataacgta aagggaaaag
     8521 ctcgattaga cggcagaatt tgtcaggggt tatgaacgaa attcataaat ctgtttgagt
     8581 gttgcgatgg gtagtgcaag ttcgatatct ccgcaattta cagtccgatg aaggaaaatg
     8641 aatatccata aaaaatatat tggtttatcc tggcatatat acctatttcg acgtatttcc
     8701 aatagtttta attaaaggca ggtcattgtt attcactctg aatagtgaat tattcactgt
     8761 ccgcagagta agaaatataa cttaggtatc tatttaatga cttgcacaaa aagctaaatt
     8821 tccccccata aataaaaata taatcccgcg cccaaccacc tgatgagtgg ctacaggcac
     8881 tggatatatt aggtggcggt gcactttctt acataaaggt atttcctttt ctgcggaaaa
     8941 ggaaatcggg aaatccccgg tttttctgac aagcagacgc cattatttgt gtctgcctat
     9001 gttcgttaat tcgttcatca ggaaattatc tcaatgtcac attataaaac aggtcataaa
     9061 caaccacgat ttcgttattc agttctgacc cgctgcgtgg cgtgggcaaa tatctctgtt
     9121 caggttcttt ttccactcgc tgtcaccttt accccagtaa tggcggcacg tgcgcagcat
     9181 gcggttcagc cacggttgag catgggaaat actacggtaa ctgctgataa taacgtggag
     9241 aaaaatgtcg cgtcgtttgc cgcaaatgcc gggacatttt taagcagtca gccagatagc
     9301 gatgcgacac gtaactttat taccggaatg gccacagcta aagctaacca ggaaatacag
     9361 gagtggctcg ggaaatatgg tactgcgcgc gtcaaactga atgtcgataa agatttctcg
     9421 ctgaaggatt cttcgctgga aatgctttat ccgatttatg atacgccaac aaatatgttg
     9481 ttcactcagg gagcaataca tcgtaccgac gatcgtactc agtcaaatat tggttttggc
     9541 tggcgtcatt tttcaggaaa tgactggatg gcgggggtga acacctttat cgaccatgat
     9601 ttatcccgta gtcatacccg cattggtgtt ggtgcggaat actggcgcga ttatctgaaa
     9661 ctgagcgcca atggttatat ccgggcttct ggctggaaaa aatcgccgga tgttgaggat
     9721 tatcaggaac gcccggcgaa tggctgggat attcgtgctg agggctattt acccgcctgg
     9781 ccgcagcttg gcgcaagcct gatgtatgaa cagtattatg gcgatgaagt cgggctgttt
     9841 ggtaaagata agcgccagaa agacccgcat gctatttctg ccgaggtgac ctatacgcca
     9901 gtgcctcttc tgacactgag cgccgggcat aagcagggca agagtggtga gaatgacact
     9961 cgctttggcc tggaagttaa ttatcggatt ggcgaacctc tggcgaaaca actcgataca
    10021 gacagcattc gcgagcgtcg ggtactggca ggcagccgct atgacctggt tgagcgtaat
    10081 aacaacatcg ttcttgagta ccgcaaatct gaagtgatcc gtattgctct gcctgaacgt
    10141 attgaaggta agggtggtca gacactttcc ctggggcttg tggtcagcaa agcaactcac
    10201 ggactgaaaa atgtgcagtg ggaagcgccg tcattactgg ctgagggtgg caaaattacc
    10261 ggtcagggta gtcagtggca agtaacgctc ccggcttatc gtccaggcaa agacaattat
    10321 tatgcgattt ctgcggttgc ctacgataac aaaggcaatg cctcaaaacg cgtgcagaca
    10381 gaggtggtca ttaccggagc aggtatgagc gccgatcgca cggcgttaac gcttgacggt
    10441 cagagccgta ttcaaatgct tgctaacggt aatgagcaaa gaccgctggt gctgtctctg
    10501 cgcgacgccg aggggcagcc agtcacgggc atgaaagatc aagactgaac tagccttcaa
    10561 accggctgga aatattgtga ctcgttccct aaaggccact aaatcacagg caaagccaac
    10621 actgggtgag ttcaccgaaa ctgaagcagg ggtgtatcag tctgtcttta ctaccggaac
    10681 gcagtcaggt gaggcaacga ttactgttag cgttgatggc atgagcaaaa ccgtcactgc
    10741 agaactgcgg gccacgatga tggatgtggc aaactccacc ctgagcgcta acgagccgtc
    10801 aggtgatgtg gttgctgatg gtcagcaagc ctatacgttg acgttgactg cggtggactc
    10861 cgagggtaat ccggtgacgg gagaagccag ccgcttgcga tttgttccgc aagacactaa
    10921 tggtgtaacc gttggtgcca tttcggaaat aaaaccaggc gtttacagcg ccacggtttc
    10981 ttcaacccgt gccggaaacg ttgttgtgcg tgctttcagc gagcagtatc agctgggcac
    11041 attacaacaa acgctgaagt ttgttgccgg gccgcttgat gcagcacatt cgtccatcac
    11101 actgaatcct gataaaccgg tggttggcgg tacagttacg gcaatctgga cggcaaaaga
    11161 tgctaatgac aaccctgtaa ctggtctcaa tccggatgca ccgtcattat cgggcgcagc
    11221 tgctgtaggt tctacggcat caggctggac ggataatggc gacgggacct ggactgcgca
    11281 gatttctctc ggcactacgg cgggtgaatt agaggttatg ccgaagctaa atggacagga
    11341 tgcggcagcg aatgcggcta aagtaaccgt ggtggctgat gcgttatctt caggccagtc
    11401 gacagtgtct gtagccgcag atcgcgtaaa agcaggcgaa agcacaaccg tgacgcttat
    11461 tgcaaaagat gcacatggca acgctatcag tggtctttcc ctgtcggcaa gcctgacggg
    11521 tgctgcgtct gaaggggcga ctgtttctgg ttggaccgaa aaaggtgatg gttcctatgt
    11581 cgctacactg acaacaggtg gaaagacggg tgagcttctc gtcatgccgc tattcaacgg
    11641 ccagccagca gccaccgaag ccgcgcagtt gactgtcatt gcgggggaga tgtcatcagc
    11701 gaactctacg cttgttgctg acaataaggc tccgaccatc aaaacgacga cgaaactcac
    11761 cttcaccgtg aaggatgcgt acgggaacct tgtcaccggg ctgaagccag atgcaccgca
    11821 gtttagtggt gccgccagca cggggacaga gcgaccttca acaggagact ggacagaaac
    11881 aagtaatggg gtctacgtgg cgaccttaac gctgggatct gccgcgggcc agttgtctgt
    11941 gatgccgcga gtgaacggcc aaaatgccgt tgctcagcca ctggtgctga atgttgctgg
    12001 tgacgcatct aaggctgaga ttcgtgatat gacggtgaag gttgataacc agctggctaa
    12061 tggacaatcg actaaccagg taaccctgac cgttgtggac acctatggta acccgttgca
    12121 gggacaaaat gtgacgctga ctctgccgaa aggtgtgacc agcaagacgg ggaatacggt
    12181 aacaaccgat gcggcaggta aagccgacat tgagctgatg tcaacggttg ccggggaaca
    12241 cagcatcacg gcctcagtga ataatgctca gaagacggtc acggtgaaat tcaacgcgga
    12301 tgccagcacc ggtcaggcaa acctgcaggt agacaccgct gctcaaaaag tggcaaacgg
    12361 caaagatgcc tttacgctga cggcgaacgt tgaggataaa aatggtaacc ctgttccagg
    12421 gagcctggtg acctttaatc tgccccgggg tgtcaagccg cttacaggcg ataatgtctg
    12481 ggtgaaagcc aacgatgagg ggaaagccga gttgcaggtg gtttcagtga ctgccggaac
    12541 gtatgagatc acggcatcgg cagggaatag ccagccttcg aatacgcaga ctataacgtt
    12601 tgtagccgat aaggctaccg caaccgtctc cggtattgag gtgatgggca actatgcgct
    12661 ggcggacggc aaagccaaac aaacgtataa agttacggtg actgatgcca ataacaattt
    12721 ggtgaaagat agcgaagtga cgctgactgc cagcccggct tcgttaaacc tggaaccgaa
    12781 tggcactgcg acaacgaatg agcaagggca ggctattttc accgctacca ctactgttgc
    12841 ggcgacatac acactcaagg cgcaagtgag tcagaccaac ggtcaggtat caacgaaaac
    12901 tgccgaatct aaattcgttg cggatgataa aaacgcggta ctcaccgcat catctgatat
    12961 gcaatctctg gtggcggatg ggaaatcgac tgcgaagctg gaggtgacac tgatgtcggc
    13021 aaacaacccc gttggcggga atatgtgggt cgacattcag acgccggaag gggtgacgga
    13081 aaaggattat cagttcctgc cgtcgaaaaa tgaccatttc gtgagcggaa aaatcacgcg
    13141 taaatttagt accagcaagc ctggtgtcta tacgttcaca tttaacgccc tgacctatgg
    13201 cgggtacgaa atgaagccag tgacggtgac cattaccgcg gtggatgccg atacggcaaa
    13261 ggacgaggag gcgatgaaat aattttaaga atatgaaagt aacattctaa taaacaataa
    13321 tggcgttgac atcttcaacg ccattattta ttgaatttcc tgacagtagt ctcgttataa
    13381 attcatttag atatagcgaa aactaaaccg gacgaacggg gataaaaatt tcgcaaacaa
    13441 tgttattgct atcaatttcg acgaaatttt cattgtaata aaaccgctca atatcactgc
    13501 cgttcactct ggctaacccc ctggcgggta attccatcag atagaggttt cgcgcccatt
    13561 cagggtagtg ctcccactca ccttcatagc ggaaagaggc atacagtcct cggttaattg
    13621 tccactcttt cggcatctct gaatcggttt gtttgcatac cgtaccggca aaagtttcaa
    13681 ccgtgagcaa atcgaccgat tttgttgacg gttcaaacgt ggagacgaaa tagatatccg
    13741 tcttaaaatt attcatacat ctggctaaat gatgacgtcg cagtttgact tcttcatccg
    13801 gtgatttatt cgacacttca atatcatatt taaatgaatt gccaaaaata ggtgtctcag
    13861 gaaagttgac taatctacac tcggtctttt gctgttgacg tattaaaaaa gaagggaaga
    13921 tatttgcgag atcccaataa tcacggtggc ggtatacacg gggagagcag ccgaataact
    13981 ttttaaattc gcggctgaat gactgctgtg aatcaaaatg caaagagagt gcaatatcaa
    14041 gcatagattt cgcggtgagc cggacaagaa tggcggcacg acaaagcctt cgtttgcgaa
    14101 tgtattctcc caaaggcaca tgcatgaaat ttcggaacag aagctggatg ttgcgtctgc
    14161 tgtagccaga tttctgtgcg atatcttcga ttgaaatagg gtgctcaaga ttgcactcaa
    14221 tccactccag gagctgctgt agaatcttct gcctgatcat cacattctcc attgattctt
    14281 cgtcatatcg cacattttcg ttatgccctg ggccctaagt gtatcaaaaa cagcaagggc
    14341 gaaggcaaaa gcactggtag cgtctgcttc gttgtccccg aaaatagaga ggtttcagtc
    14401 ctgcattatt aatgaatttt ttgcataagt gatatcaaaa tccacgtact aatttgaggt
    14461 tacgttttaa cgtagactca ttgttcatgc ctaatggagg gactgacagt ggaattttcc
    14521 gtattaagta acaatctgaa aatgccgatg atgggatttg gtgtttttca ggttaccgat
    14581 aaaaacgtgt gcaaacagtc agtgttgaac gctatccgca caggttatcg actcatcgac
    14641 actgcagcgg tatacggtaa tgaagatgcc gtcggtgaag ccgttcgtga agctatatct
    14701 gaaggtttat gtacccgaga tgagttattt ataacgtcta aattgtgggt gcaggatatg
    14761 ttgaatcagg atacggcagc agcaggtatt gaagcatcat taaaaaaatc gggactagag
    14821 tacttcgacc tctatttatt gcaccaggct atgcgcgatt atttcagtgc gtggcgtgca
    14881 ctcgaagatg cctatgaaga gggcaaatta aaagcaattg gggtttccaa tttctatcct
    14941 catgttctgg cgaacttttg tgaaacggta agagttaaac cgatggtcaa ccaggtcgag
    15001 ttgcatccct attttgccca accagaggcg ctggcaacca tgaagtatta taacgtgcag
    15061 cctgaagcat gggctccgtt aggtggtgga cgacataaac cctttgaaaa taatctgctt
    15121 cagagtattg cagatgccca tcaaaaatcg atttctcaag tcattctgcg ttggaatatt
    15181 caacgggaag tggtcgttat tccgaaatcg acacatcaac agcgtatcga agaaaatttt
    15241 gctatctggg atttctcact gacagagaaa gaaatggcac aaattagttc gcttgatttg
    15301 ggttatgttg gggagtcggt aaaacatttt aatcctgaat ttgttcgtgg ttgtcttgct
    15361 gtaaaaatac atgattgata ttaatcatat attttacctg agacgacaag aatcttttaa
    15421 caggggagtg atattgatct tcactctgtc atatctccgg taatatggcg tcaggcttca
    15481 tcatcagaaa gggggcctga cgcctgaaaa agtgaacaac agacagtgtt cggattatca
    15541 atattcagtt tttaacgttg aactggattc attactgggt tgtttaagaa tttcccgcgc
    15601 cgaatctgcc attatcatga cagcacctgc cagcatcaga gtatctttca ataccaggcg
    15661 accagcaccg gataaataag ggaaaccatg atgagcgtca cccaatgcgg gtacccatgc
    15721 ctccggggtg gtgattaaaa atgagagtgt taccagcggc gtggtaaatg ccatcagtcc
    15781 tcccaataaa cctaaccagc gattgacagg attagccaaa accagcagag caataatcac
    15841 ctccacgacg ccaagaccgt tggaaaaacc ataggtatta ttggccgttt gccatgccct
    15901 tgcttctggt ttgtattcgc cttcgtgagt cagatactgt ttatagtctt ccgggtgttc
    15961 ataaaagaac gacattagtg gactgtttgc gacgaatggt gtaatgctgt ctgcctcgta
    16021 agggacaaac tttaataacc caatccacat aaaaaaaatt gcaatactca gacgaatcaa
    16081 tgtcaggcca attttatctc cccgacttaa caggtgcagg tatttttcca ttgtgaacat
    16141 ccttatttat acaatactgc tgttgcggtt gaattattat caattcgcat cgatgtgact
    16201 ttccaggatg aagcattata ttttctggct atatcagcaa tggtatcctc cagaactgtc
    16261 ggtgatgctg atactgacga gacgctgata tgtttcagaa ttattttatc atctgcattg
    16321 gtggaaaatg ccataacgcc agataatagt gttgcaaata aaacagattt tttaaacata
    16381 taaaatacca tcaatgaagt gattaagaag ttatagagta acagggaatt aataagattc
    16441 ttttctctga gacgccagaa tatttgttct ggcgtctgat tttgagttta tttgactaat
    16501 gaaaatagat cattgagtga ttcgctcatc gacggatgag taaatatctg atcgcgtaat
    16561 atgctataag gcagcccggc atccatcacc attttcacta tattgatcat ctcgtgggag
    16621 tcaacacaca gcagtgatgc ccctaatata cgttgggttt tattatcaac aatcgctttt
    16681 aataccccac gagtatcatt catcactctg gcacgcggaa ttgcagctac aggcaatgtc
    16741 accacctgaa tatcagcacc actctctctg gcctgttctt ctgtcatacc aaccctggac
    16801 agtggcggtg tcatgaatac ggaataaggc acatttttcc gatcatcagt actacgtctg
    16861 ccttctccca gcaactcatc acgcacaatg cggtaatcat ccagtgaaat ataagtaaat
    16921 tgcagcccgc cggtaacatc tcccatcgcc caaatattgt ctgcggtggt atgtaattgc
    16981 ttgtcaacga caattgcccc gcgctcgttt actgcgatac cggcattttc tggatgtaac
    17041 gaagcggtag ccggttgacg accggaagct attaacagtg catccaccgc cagttgggcg
    17101 tgctcgctat gcacttgcac ttgattttca tggtgactga ttcgctccac atgggcattg
    17161 aggataatat cgacgccctg atcgcgtaaa atcgtcgcga tattatcagc aatatcccga
    17221 tcttcccgag gcaaaaacag cgaagctgct tctaaaatgg ttaccttgct gccaaaatta
    17281 gcgaacatag aggcgaactc aacgccaata tatccgccgc ccaaaatacc taaatgccca
    17341 ggcaattctt ttagattaag taatccggtg ctgtcatata ctcctggcgt ggtggtaatt
    17401 ccaggaattg gcggaaccac ggtttgtgca ccggtattaa taaaaatttt ctcgccatga
    17461 atttccagat ttgcctcagg ccgatgaaca cgcaggctat gattattgat aaactccgcc
    17521 tggccgtcga tcacgtcgat attgggcata tccgcaagat tatgaaaatt cttattacgt
    17581 aaaaaattaa ccacttcatt tttacgctgt atggcacgga caaaatctgt gtgctgctgt
    17641 gcgtcatgaa ccaatgtttt ggttgggatg cagccgatat taatacaggt cccgccatac
    17701 attgcatttg attgttcgat gagagccact cgccaacctg cttttgccag cgtgacggct
    17761 aatgtttttc cagccttgcc aaaaccaata atcactgcct gatatttatt catgatgatt
    17821 tcctgtcgaa acgcgttcat ttgacatcat gactatagac gcaaaagagc aaataccatt
    17881 tcttcacaac gcttgatatt ttgtcttatc gtctggtatt ttctttttct taatcagaat
    17941 cacaaaacaa aaatcatgat taaccatttg atggtattag attagttatt tactaagatt
    18001 gttggtgttt gtaatcaaaa accactcagg agtctgatat ggatgccctt agccgtttgt
    18061 tgatgcttaa cgctccacaa ggaacgatcg ataagaattg cgtgttagga agtgactggc
    18121 agcttccaca tggtgccggg gaattatcgg ttattcgttg gcatgcgtta acgcaaggag
    18181 cggcgaaact ggaaatgccg acgggagaga tttttacatt acgcccggga aatgtggtcc
    18241 tgctaccaca aaattcagct catcgcctga gtcatgtcga taatgaatcg acctgtattg
    18301 tctgcggcac tcttcggttg caacattcgg tgcgttattt tttaacgtct ttgccggaaa
    18361 cgctgttttt agcaccggtt aaccacagcg tggaatataa ctggctgagg gaggcgatcc
    18421 cgtttttaca acaggaatcc agatcggcga tgccgggagt ggatgcactg tgtagccaga
    18481 tctgcgctac attctttacc ctcgcggtgc gtgagtggat tgcacaggtt aatactgaga
    18541 aaaacattct cagtttgctt ctgcatccac gtcttggtgc ggtaatacag caaatgctgg
    18601 aaatgccagg acacgcctgg accgtcgaat cgctggccag catcgctcac atgtcccggg
    18661 caagttttgc ccagcttttc cgtgatgttt ccggaaccac gccgctggct gtattaacaa
    18721 agttgcgtct acaaatagcg gcccagatgt tttcccggga aatgctccct gttgtggtga
    18781 tcgctgagtc agtaggctat gccagtgaat catcttttca caaggcgttt gtccgcgagt
    18841 ttggttgtac tccgggagaa tatcgggaaa gggtcagaca gcttgcaccc tgaataaaac
    18901 cgccagaaat cagggcaaag ataatccgca ttccgggagt tgtgagcctt tcaactattt
    18961 ctatttccaa cggtggtttg ggctttatat atttttttct gatggactct agctcagttt
    19021 ttaaataaaa atgcaaacta aattgcttca attaaataaa accccgacta ttacttgata
    19081 cgtgattatt gtcggtgatt tttttgtaca ccatcagtgt atatctcaat aacccctgaa
    19141 taagtagctc tgaataggta taagggatgt agccttttta aatggatttc ttatgccaaa
    19201 aatgaacgtc gatttcccca taaaatgtga gcgatgccga aagaaataaa attagttatt
    19261 gcatttgaag tttggatgaa agattatcat ttgtcataca aatgaggggt ggtatgttgc
    19321 tagccaatta aaaaagaacg ccatatttat tgatgattga tcccccggtg ggggggaaac
    19381 atcgggcata aatgggcatg aagtaatgga gtattagttg tgaatgtcaa tttctttgtc
    19441 acctgtattg gtgacgccct gaaatcaaga atggcacgag actccgtgct gctactggaa
    19501 aaactcggct gtcgcgtaaa tttcccggag aaacagggat gctgcggtca gcctgcgatc
    19561 aatagcggtt atatcaaaga agcgattcca gggatgaaaa atctgatcgc cgcactggag
    19621 gataacgacg atcccattat ttcaccggct ggctcttgca cctatgccgt aaaaagttac
    19681 ccgacgtatc tggcggatga acctgaatgg gcatcacgtg ccgaaaaggt tgccgcgcgt
    19741 atgcaggatc tcacctcttt tattgttaat aaattagggg tagtcgatgt aggtgccagt
    19801 ttgcaaggga gagctgtgta tcacccatct tgtagcctgg cccgtaagct gggagtgaag
    19861 gacgagccac ttacgctgct gaaaaatgtg cgtggactgg agctgttgac ctttgctgaa
    19921 caggatacct gctgcggatt tggcggcacg ttctcggtca aaatggccga aatatccggc
    19981 gagatggtga aagaaaaggt tgtgcacctg atggaagtcc gccctgagta tttaattggt
    20041 gctgacgtga gttgcctgct gaacatcagt gggcgattac aacgggaagg gcagaaagtc
    20101 aaagtgatgc atattgctga agtgttgatg agccgctgag gatataaaga tgtcgatcaa
    20161 aaccagtaat acagatttta agacacgcat ccgtcagcaa attgaagatc cgatcatgcg
    20221 caaagcggtg gcaaacgcgc agcagcgtat tggggcaaat cggcaaaaaa tggtcgatga
    20281 attggggcac tgggaggagt ggcgcgatcg ggccgcccag atacgtgatc atgttctaag
    20341 taatctcgac gcttatctgt accagctctc agaaaaagtg acgcaaaacg gcggtcacgt
    20401 ctattttgca aaaactaaag aagacgccac tcgctacatt ttacaggttg cccaacgcaa
    20461 aaatgcccgg aaggtggtga aatctaaatc gatggtgacc gaagagattg gtgtcaatca
    20521 tgtgttgcag gatgctggca ttcaggtgat tgaaaccgat ctgggtgaat acattctcca
    20581 gctggatcaa gatccgccat ctcatgttgt ggtcccggca attcataaag atcgccatca
    20641 gatccgtcga gtgctacacg aacgtctggg ctatgagggg ccggaaacgc ctgaagcgat
    20701 gaccttattc atccggcaaa aaatccgcga agatttcctc agtgctgaaa taggtattac
    20761 cggctgtaat ttcgcggtgg cagagacagg ttcggtatgc ctggtgacca atgaaggtaa
    20821 tgcgcgaatg tgtaccacgc tgcctaaaac gcatattgca gtgatgggaa tggagcgtat
    20881 tgcccccacg tttgccgagg tagatgtatt gatcaccatg ctggcgcgca gtgccgttgg
    20941 tgcacgtttg acgggataca acacctggct gacaggaccg cgcgaagcgg ggcacgttga
    21001 tggtcctgaa gagtttcatc tggttattgt cgataacggg cgttctgagg tgctggcctc
    21061 tgaatttcgg gatgtgctgc gctgtattcg ctgcggggct tgtatgaata cttgtccggc
    21121 atatcgccat attggcggtc atggatatgg ctctatttat ccagggccaa ttggtgcggt
    21181 gatttctccg ctacttggcg gctataaaaa ttttaaagat ttaccctacg cctgctcttt
    21241 atgcactgct tgtgacagcg tgtgtccggt gcgtattccg ctgtcaaaac tgattttgcg
    21301 tcatcgtcgg gtgatggctg aaaaagggat caccgcaaaa gcagagcaac gggcgataaa
    21361 aatgttcgct tatgccaata gtcatccagg attgtggaaa gtcgggatga tggccggcgc
    21421 tcatgcggca agctggttta tcaatggcgg caaaacacca ctcaaatttg gcgcgattag
    21481 cgactggatg gaagcacgcg atcttcctga agctgacgga gagagtttcc gtagttggtt
    21541 taagaaacat caggcgcagg agaaaaagaa tggataatcg aggcgaattt ttgaataacg
    21601 ttgctcaggc actgggtcgc ccgctgcgac ttgaaccgca agcagaagat gcgccgctta
    21661 acaactatgc taacgagcgg cttacccaac ttaaccaaca gcagcgctgt gacgcgttta
    21721 ttcagtttgc cagcgatgtt atgttgacgc gctgtgagct gaccagcgag gcgaaggcgg
    21781 cagaagctgc aatacgtctg tgtaaagagc tgggagatca gtcggtcgtg attagcggtg
    21841 acacgaggct ggaggaattg gggattagtg aacgtttgca gcaggaatgc aatgccgttg
    21901 tttgggatcc ggcgaaaggt gccgagaata tctcgcaggc cgagcaggct aaagtgggtg
    21961 tcgtgtatgc tgaatatggt ttaaccgaat cgggaggcgt ggttcttttt tccgccgccg
    22021 ggcgcgggcg ttcattgagc ctgctcccgg aatcttctct ttttatcctg cgtaaaagca
    22081 ctatcttgcc gcgtgtagcg caactcgcag aaaaattgca tcagaaagcg caggccggtg
    22141 aacgaatgcc gtcctgcatt aacatcatta gcggccctag ttcaacggcg gatattgagc
    22201 ttatcaaagt cgtcggggtt catggtccgg tgaaagcggt gtatctgatt attgaggatt
    22261 gctgagagag atatgagatc tgaacataaa cctgaagaag gcatacgagg ttgaaaatag
    22321 cgttaaggac aacctgactt aaccccgttc cgtcatggtt atgctgctct tctgtaaata
    22381 tcctggcatt agtaatgcca ggatatttac agtgaaaaag acaggcatcc attcctgaaa
    22441 acgggttccc ttcccggtca ttgtcacttc agctttatac aggcactctc tatcagaatg
    22501 ctgtttatta ctacccagag ttttgttggt attcatctac catttttttg acaaggcaaa
    22561 acattacaga aattacaatg cttagaaaaa atcccagagc caggcaaatg gcagcagggt
    22621 atttattttt tatttctggt gagcttatag ttacacagcc atcaccgttc ggatacaatg
    22681 accagttcct ggtcaggtat atcatttttg tataaagctc actgttaatt gctgaaggtg
    22741 tttgtatctc atcgagaact tgcctgccat tatggtattc tgacgcatac catttgataa
    22801 tagacgcctg cacgcgagat atttccgctt cagcagttgg taaatctctc ttatctcctg
    22861 accattccag ctttatttct ctgttcttga catctgggct ggtgtgaaaa aatgtagtaa
    22921 ttcgctgtat aacatcttca gtcattgtgg tctgacaggc aaagttatta ttgctatcac
    22981 agttaacaag tatgcctggt gttgtctggt ttatttttag gctgtaactc acttttatat
    23041 tatctttatt gatatacaga a
//
LOCUS       DAZUFB010000071        22930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZUFB010000071 DAZUFB010000000
VERSION     DAZUFB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.071"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..73)
                     /locus_tag="ACHY3M_003155"
     CDS             complement(<1..73)
                     /locus_tag="ACHY3M_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318023.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQ"
     gene            complement(93..539)
                     /locus_tag="ACHY3M_003156"
                     /pseudo
     CDS             complement(93..539)
                     /locus_tag="ACHY3M_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(541..1488)
                     /locus_tag="ACHY3M_003157"
     CDS             complement(541..1488)
                     /locus_tag="ACHY3M_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4318024.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(1485..1661)
                     /locus_tag="ACHY3M_003158"
     CDS             complement(1485..1661)
                     /locus_tag="ACHY3M_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318025.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTAVEAYCDEWGDE"
     gene            complement(1654..1836)
                     /locus_tag="ACHY3M_003159"
     CDS             complement(1654..1836)
                     /locus_tag="ACHY3M_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318026.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEGQNG"
     gene            complement(1930..2286)
                     /locus_tag="ACHY3M_003160"
     CDS             complement(1930..2286)
                     /locus_tag="ACHY3M_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318027.1"
                     /translation="MEMKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRRNVEKLIQIE
                     RNAKARIRRAEKSRTE"
     gene            complement(2264..2473)
                     /locus_tag="ACHY3M_003161"
                     /pseudo
     CDS             complement(2264..2473)
                     /locus_tag="ACHY3M_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2795..2902)
                     /locus_tag="ACHY3M_003162"
                     /pseudo
     CDS             complement(2795..2902)
                     /locus_tag="ACHY3M_003162"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(2986..3225)
                     /locus_tag="ACHY3M_003163"
     CDS             complement(2986..3225)
                     /locus_tag="ACHY3M_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318028.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHEGGNQ"
     gene            complement(3218..3421)
                     /locus_tag="ACHY3M_003164"
     CDS             complement(3218..3421)
                     /locus_tag="ACHY3M_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318029.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(3418..4296)
                     /locus_tag="ACHY3M_003165"
     CDS             complement(3418..4296)
                     /locus_tag="ACHY3M_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFS4318030.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELRQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYSNLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(4404..4838)
                     /locus_tag="ACHY3M_003166"
     CDS             complement(4404..4838)
                     /locus_tag="ACHY3M_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318031.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYDEKHEEHIQNGCINKCQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTMLEF
                     NNINHSGRDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(4915..5736)
                     /locus_tag="ACHY3M_003167"
     CDS             complement(4915..5736)
                     /locus_tag="ACHY3M_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFS4318032.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAIRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(5800..6147)
                     /locus_tag="ACHY3M_003168"
     CDS             complement(5800..6147)
                     /locus_tag="ACHY3M_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318033.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(6222..6809)
                     /locus_tag="ACHY3M_003169"
     CDS             complement(6222..6809)
                     /locus_tag="ACHY3M_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFS4318034.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTVIVQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANIVSTLKDKDNRSKNVNPNHTDDPGYWQYGSNSF"
     gene            complement(6816..7499)
                     /locus_tag="ACHY3M_003170"
     CDS             complement(6816..7499)
                     /locus_tag="ACHY3M_003170"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFS4318035.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEAMAEIG
                     FVFGEQWGG"
     gene            complement(7496..8446)
                     /locus_tag="ACHY3M_003171"
     CDS             complement(7496..8446)
                     /locus_tag="ACHY3M_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS4318036.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAMTEINKTWDDLLPVCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(8463..8744)
                     /gene="gamL"
                     /locus_tag="ACHY3M_003172"
     CDS             complement(8463..8744)
                     /gene="gamL"
                     /locus_tag="ACHY3M_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFS4318037.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(8765..9046)
                     /locus_tag="ACHY3M_003173"
     CDS             complement(8765..9046)
                     /locus_tag="ACHY3M_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4318038.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(9058..9270)
                     /locus_tag="ACHY3M_003174"
     CDS             complement(9058..9270)
                     /locus_tag="ACHY3M_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFS4318039.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(9341..10015)
                     /locus_tag="ACHY3M_003175"
     CDS             complement(9341..10015)
                     /locus_tag="ACHY3M_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS4318040.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            complement(10271..11044)
                     /locus_tag="ACHY3M_003176"
     CDS             complement(10271..11044)
                     /locus_tag="ACHY3M_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4318041.1"
                     /translation="MGPTSVGLVPLIASCENPVQSTASELLTSSGGYQSPIKEAAIMA
                     TIPVLSQPEITISNGRAVTTSFAVAEYFCKRHDNVLRAIANIECSEKFNALNFEDVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(11673..12626)
                     /locus_tag="ACHY3M_003177"
     CDS             complement(11673..12626)
                     /locus_tag="ACHY3M_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFS4318042.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(12623..14092)
                     /locus_tag="ACHY3M_003178"
     CDS             complement(12623..14092)
                     /locus_tag="ACHY3M_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFS4318043.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSAIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(14187..14900)
                     /locus_tag="ACHY3M_003179"
     CDS             complement(14187..14900)
                     /locus_tag="ACHY3M_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFS4318044.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            14996..15199
                     /locus_tag="ACHY3M_003180"
     CDS             14996..15199
                     /locus_tag="ACHY3M_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4318045.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGRVEAEEVRPFPAFVRGDD"
     gene            15254..15523
                     /locus_tag="ACHY3M_003181"
     CDS             15254..15523
                     /locus_tag="ACHY3M_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318046.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            15731..16108
                     /locus_tag="ACHY3M_003182"
     CDS             15731..16108
                     /locus_tag="ACHY3M_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318047.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTLLESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRSRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            16102..17622
                     /locus_tag="ACHY3M_003183"
     CDS             16102..17622
                     /locus_tag="ACHY3M_003183"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4318048.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFHPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            17612..18583
                     /locus_tag="ACHY3M_003184"
     CDS             17612..18583
                     /locus_tag="ACHY3M_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFS4318049.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVIDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            18583..19032
                     /locus_tag="ACHY3M_003185"
     CDS             18583..19032
                     /locus_tag="ACHY3M_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS4318050.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            19040..19603
                     /locus_tag="ACHY3M_003186"
     CDS             19040..19603
                     /locus_tag="ACHY3M_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFS4318051.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRNLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHNRHRW
                     TVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            19600..19794
                     /locus_tag="ACHY3M_003187"
     CDS             19600..19794
                     /locus_tag="ACHY3M_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFS4318052.1"
                     /translation="MTFTVKTISDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            19787..20221
                     /locus_tag="ACHY3M_003188"
     CDS             19787..20221
                     /locus_tag="ACHY3M_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS4318053.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            20469..20621
                     /locus_tag="ACHY3M_003189"
     CDS             20469..20621
                     /locus_tag="ACHY3M_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFS4318054.1"
                     /translation="MPASPSGWLFYVRSVKAAMSLGRRAIGVELESGRFEQTVREVQN
                     VVSQNG"
     gene            20662..20737
                     /locus_tag="ACHY3M_003190"
     tRNA            20662..20737
                     /locus_tag="ACHY3M_003190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20695..20697,aa:Ile,seq:cat)
     gene            20745..20821
                     /locus_tag="ACHY3M_003191"
     tRNA            20745..20821
                     /locus_tag="ACHY3M_003191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20779..20781,aa:Arg,seq:tcg)
     gene            20835..20911
                     /locus_tag="ACHY3M_003192"
     tRNA            20835..20911
                     /locus_tag="ACHY3M_003192"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20869..20871,aa:Arg,seq:tct)
     gene            21442..>22930
                     /locus_tag="ACHY3M_003193"
     CDS             21442..>22930
                     /locus_tag="ACHY3M_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4318055.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIR"
BASE COUNT         5598 a         5451 c         5725 g         6156 t
ORIGIN      
        1 tttgatattt accatttgta cactggcgac aatcacagta gagatgcatg gtgtaacttg
       61 cggcaatagc catatccccc tcctttaccc tgaagcatgg cgtcgctccg ctctatacca
      121 tccagcgcga ttcgcagtgc ctgaattgtg gtagtgctat cgtttggggc tattccatat
      181 cgctcgaata cagctaaatg gttgcgcata atctcaggcg taagctcttt gtaagcataa
      241 gcaagaggct ctgatgcatt atccggcaca accgacgcag gcgcggcagc ataaacagga
      301 ataacgtccg cttgctcttt attgctttca tccgttaaag cccagaataa tttcccggcc
      361 ggatgtttga aaatataagc aactggttct gcactatcag cttcgcgccg cttctgtagc
      421 tctgctgcca tcgctctcac gacttcaact ggtgcccttg cagcaaactc catgttggtg
      481 atcagctcat taagaaattg ctcgctggga tactgtttct tatcggttat agtggtcata
      541 tcactctcct ttgatgcgaa tgccagcaag ccagtttctt atgccgatat attcagcgtt
      601 cctgaaaccg ctttttacat atataaatgg caagcgaaga ttgtgaccat tgactgccag
      661 gtagtcttta caaccctgtt cggtgaaaca gcaggtaacg aattcatcaa tatctttcac
      721 agcaacgcgc cgccattttt ctggtggctc tcgaaagttt tcatgaagta gctcgagacg
      781 acgactatgg cgtttattgg cttcattgcc atcttcgtca acccagacaa tccggtcatg
      841 gtcataatca gcatcaacaa cgatttcgcg cttttgatac acacaaaaca tgggatctga
      901 cgttattcga ttgtcctgtg ttcgaatatt ttcaccgatg atgccaaacg aatctggtgc
      961 agattttgtc tgcatctctt cgatacgttc agccatcgca gcacactctt caaagttgct
     1021 taatgctttt cgctcccatt cggcgcattg tttttccagt tctgctatgc gcttacttcc
     1081 atccgcgatt actccctcgt aatattcacg ctgctcgttg agttttgatt ttgctgcttc
     1141 aagctcaaca cgcagcttcc caaccgtaag agcaatttcc tcgttctcct ggtcgcggcg
     1201 tttgatgtat tgctggtttc tttcccgttc atccagcagt gccagcgcaa catttggatt
     1261 aaaggcagca ataaattcag cgtttgcata agcctgaaca tctgtttcaa ccaggcagtt
     1321 aacatgacat tctgcaatta cgccaccggg ttctcctttc catttttgac aaacaaaaac
     1381 tcctgttata ttcccatgct gattgcccga tgtatgccct acgatgtagc atcctttagt
     1441 tgctttctct gctgcttcac gcagcgcctg atagtcaatc tcgctcattc atcgccccac
     1501 tcatcacaat atgcttcgac cgcagttttc cctgcttcat aatcatcacg ccatgcttca
     1561 gcatcagcgg cacttccacc gcgtaactct gcataatcca ttaacagttc atgccattct
     1621 tcaaaactgg cgttatattt agttgaacca aaatcagcca ttttgtcctt cctcttcatc
     1681 ttttatttcg tggtatgagt aattgcagta gttaaagaaa atttcttttg cttcgtcatg
     1741 aatttcatca ggtgttgcgt catcatctac ttcgaataca tcctcaaaat ctccaccagc
     1801 tattcccgtt tcaataatta ttttgaattt tcgcatttca ctaccgccat ttcgaacggc
     1861 ctcctgatgt tctgagggtg cagaaatccc tccggttaag gattaaattt ttaacagtgc
     1921 taaatttaat tattcagttc ttgatttttc cgccctgcgt atccgcgctt tcgcgttacg
     1981 ctcaatctga attagctttt ctacatttct ccgcctttcc cgttcctcct ggcgcaagag
     2041 ccttacatca tctgccagtc tggtttctct tttcgccaca gagagcatcc agtcaaatgg
     2101 ctccacaact gcaccgcaga ttttacagcg gacctgacgc tctttttcgt caacccggac
     2161 agaggcgtga tgacaatatg gtctttccga tggctcataa agaaaattaa cctgattacg
     2221 tgggtcatcc tcttttaccg gaaataaaac aatattactt aactcatctt ctggtttcat
     2281 ttccatgctc ctctcctttg atgcgaatgc cagcaacacg tagtgcatgt tctaagtcag
     2341 ccagataaag ccagcagcca ttttctttag gtatcatgac gtggcgctca tcatcattta
     2401 tcggatgtcc atatcgaagg ccgtagcgag tcggcaaatg aacttcccgc gtttccagct
     2461 ctttaacgcg ttcctccagt tcgtagaccc tgcattgttc tctatcatca atcagatata
     2521 acccaagaca ttcgctttct acccaaccgc caaaatcatg atcgtaacgc tcacatgaaa
     2581 actcaccgtc accgtccttt gttggaatgg tgtaactatc taatgggcca ccatatgtcg
     2641 gcacatttcc caatgttgga tgctcaatcc acatgaaaaa tgtacgtccg gttattgggc
     2701 aaatatctgg ccgccattgg ttacgaacag ccttggtttc ggataattct tcagcgtgtt
     2761 gttttacttc ctcaagctca acacgcagct tcccaaccgt aagagcaatt tcctcgttct
     2821 cctggtcgcg gcgtttgatg tattgctggt ttctttcccg ttcatccagc agtgccagca
     2881 caacctgagg tgtgactttc atacgaaatg ccagcaattt ttgaggcgtt gctactgttt
     2941 caattgctac tgccgcctca cgcagtgcct gatagtcaat tttgctcact ggttgcctcc
     3001 ttcgtggatc tgcgctgcga tacacgaaaa aaaagactcc cgcgtatgac tgttaagagc
     3061 tggtgcgaac gccgcgttaa gaacggcagc atcacagccg tcatcgatat agagcgcaat
     3121 ttttttctcc aggcgcgctt tggcttcctg caactgcata ccccggcacg tacgcgggat
     3181 atactcagca atttgagcga tagatttttc gttctgttta aacatgcttc acctcgacag
     3241 gcttgattgt gtcgatcagc agtctgcggc gcgtattttc tgcaaagtgg cggcgtccgg
     3301 tttctttgtg gtaaaactcg tttttgccga cgacccacat ccgctctgtc tggtgaagtt
     3361 tttttacctg cgggccgtct ttggtgatca caatgccggt atgggttttt acgattgtca
     3421 tgccactacc tcttcgaatt tcaactccaa ttggtcaccc cagatttcac atgattcgga
     3481 acacgaacca gtatcaaacc gcttggccag caccatcgct tgatacaaat tgctgtagtc
     3541 gctgtcggca tacatcctgg caatcccgtc aagcgtcagg tgaccacggt acataacgtc
     3601 tttacctgtt ctgcgatgac catccctgac gtgtttgcct gtaaccagtt cattaaaaac
     3661 ccgcatcaga ccaggttcgt ctttacatgc aagccccagc ttttgcgttg actttttgat
     3721 gcagaaaaca cagttcccga ggtgctccgg gatttgcaaa tcaaaaggtt gttttcgcca
     3781 ccaccggata acatccgact tatcaaaatc tgacagttcg gcaagatacc ggacgcccgg
     3841 tttcggtttc agcctacggg gttcgtctgc acgaataccc agccacgtga tgtagttccc
     3901 ttgtccgaaa tggtcatcgc agtattttgt gaagggggtg agttttaatc tgtcagtgca
     3961 gaacgcgccg ccgatgtatg gcgtgccata tttttttacc atgtccataa acggtttaag
     4021 caccggcatt cgcgtctgaa tatcctttgg ttcccattct gtataaccat ttggctgcct
     4081 aagctctgga tttatatcga cctgcaacac ggttagcgga atatcccaga acttcacaac
     4141 ctcccgaata aatcggtacg tcagcgggtg ttcacatccc gtatccatga gtatgtaatg
     4201 aacgttttca ccagcttttc tgcgttgctc cataaggtgg acgaggtacg ccgatgtccg
     4261 accgcctgaa aaactcacaa catgatgagt gctcatttct tcgtctcgtt acggggaggg
     4321 ggttaaatag gtggtaagct ggaattgccg gatgtttacc cgtgtccggg gtacgacctc
     4381 acgttgcagc gtacgagaat agactattgc tcagtatcgt ttgatgtcgg ctgaggatat
     4441 tcgccttgct cttgtaacag agttagaaga atggaaaacc cctcacgatg tgatgattga
     4501 tgagtgtctc tcccggaatg attgatgttg ttaaactcca acatcgtgct cctggcgata
     4561 gcctcaacgg tattccagaa tactaataca tctgttttct gctctgtttt atcagcaaca
     4621 ggaagtttga tattttgttg acatttatta atgcaaccat tttgaatatg ttcctcgtgc
     4681 ttttcatcat acagcccgac aaaaacatct cgcacgttac gggatatatt ttccagattc
     4741 gcctttggtt cctttgttaa atcaagcacc gataaatggg ctttaagcgt atccgcagct
     4801 attttctgaa tatcctctgg taaatcttta aattgcatag tataccctcc aggaactgtg
     4861 tattttctcg tgaaatatga gcgatatgtc gtgacgtccc tgtcacagat ttcatcacac
     4921 tgagaacgtg ccgatgaagg ttaagatatc ttcttcttcg aaacgctctt tcagcaggtc
     4981 acggaactct ttggcaattt cttcttccgc agcttcaaca cgcacgatac gtagcaccag
     5041 aacaggttgt tcgcttgtca gaatgcttaa tcgcagcgtg aattccctgt tgcccaggcc
     5101 ctcgtatggg atgcattcaa agcggaaagt ggcaggcatg atttctttgc ttttagcctc
     5161 cacggactcc atgactgagc ggctaccgct gaagttttgc tcttcatatt cagcactgcg
     5221 ttttgcgtcg atggtaagac gacggatagc agagatagcc tgtttgatat ccagcacatt
     5281 accgtcgctg tcataggctg tgaggaagtc ggcccagtct tccagccatt cagcgagaga
     5341 tttctggagt tgtcttttgc cgtccacgtc acgtaatgcg cgatatggtg atgtggcttt
     5401 cagttccagc agtgccttgt tgtcagcgtg tccaggttgc tccagagtgc cgatattgaa
     5461 cacagtgact gctttcatcg tttccgcgtt aatgaagcag cgggaatgct catcggcata
     5521 ccctttgctg tattcaacaa agtcatcaat actggttgtg gtcatgactc cacggaaacg
     5581 aaaacgttct gtcatgaatc gttccagact ttcgatattt acattgtccg gcaataacgc
     5641 aacagggcat tccgtaagcg gcaaattttc accagaaaaa gcagtagtgg tgagttttac
     5701 aatttcttta atggcattgc tatctaactg agacatgtat atttaccttt aatttatgga
     5761 tttagggata atgcgcgagc gttttaaggc ggggattatt cagcgtaaaa cagcgtcatg
     5821 ttgaccctgt ttatcgaaaa gctgtccctg gtctttctgg aacaaagtaa gctcaccacc
     5881 tttatttaca aacatcgggg tgttagttgt gtcttcttct gatttactac cacgcattgt
     5941 cgggcgtgtg aattttagtt tatgggaaac tgatacgcga ttttcatcaa gtgaagataa
     6001 ttcgaattca acacatacct ttcctttttt gtttgtgctg tttacgccaa aggcaacttc
     6061 acttaatacc gcaccaagtt tgttaacaaa taccccgccg tcgaggtcgt taatgaaaac
     6121 attaacatca gtttgatgct ggttcatatt aatatctccg tgtagaggtg tgttaaggct
     6181 tcagttgttc actgtattta aagcctggtt ttgtttgtga attaaaaact attgctgccg
     6241 tattgccagt aacccgggtc atccgtgtga ttcgggttta cgtttttgct gcgattgtct
     6301 ttgtctttga gagtggaaac tatatttgct acagtttctg gtttctttcc ttcagctttt
     6361 tctttcagtg tctgccctgt ttctgcaatg aatggaatgc ggatttccat ctggtaaccg
     6421 tctgcgccag tctttctgtt tgtggttaat actttctgga ggacgagtcc gattttcttt
     6481 ccgtgaaact caggtgcaac atatttactg acggaaacca tatgctgcgt taactgcaca
     6541 attactgtgc atcccatcat ggcgtgaata atgctcgccc caaatttatt ttcagtgccg
     6601 tcattcttct gaacacaaac actgagatat tggattttcc gtccgtcgtc ggattctcca
     6661 gaaaactcaa tgaatcgggc acctttttct gattgcttga gctctgcttc ggtgatgtta
     6721 atgatatgcg cacccgtttc ggtaataaaa ccaccttgtc cggcggtcag tgctgattct
     6781 tcgttataag taaaaatcac ttcatccatt agttattaac ccccccattg ttctccaaat
     6841 acaaaaccaa tttccgccat tgcttcgtcc attttgtcga tgaactccgg caccatctca
     6901 tcaaaactcg ccatgtactt ttcatcccgc tcgaccacga cataatgcag gccttcacgc
     6961 ttcatgcgtg ggtcgtagtt ggcgaagtac caggcgtcct tgtctgtaac ccacatgctg
     7021 aattgcacct gggccatgta agcaggcttg atagcgtcaa agccgccaag ccggaatttc
     7081 atgaagtcgc gggaagtaaa agggcattta agctcaagac cgttaccatc gctgcataaa
     7141 ccatcgggag agcaggcggt gcgcatgctt tcgtcacgaa aaattatggg tgtttccgag
     7201 acggtaacat ccgcgataaa ttcaaagagg gcacgggcat catcttcata ttgctttccc
     7261 catgcgagcg acttagcgtt aacttccggt gccacgccag tacaaacttc agccagcagg
     7321 gtgtggaagt atgacatttt tgtatcaggc catttgctgc cggaacgtgg ctttgctatc
     7381 acattgtgaa cttctgaagc agtgataaca ccgagtctca gcttgtgcca tccatcatcg
     7441 ccctggtcga ggctggtaat gtccacgcca gttcgctgga gaataatttc tggtgtcatg
     7501 acacggcctc gctgttattt tccgtggtgg cgtgaatttt cgcttccgcc gtggccttgt
     7561 ttctggcagc tttctttttg acaaaatcaa gcgttttgac agcttcttcc tgactgagat
     7621 attcatgtga tgcaatcgtg cgacggaatg ttttggaaca tacgggaagc agatcatccc
     7681 atgtcttgtt aatttcggtc attgcctgag tgatctcatt gataatctca tctgatgcgg
     7741 gagtgacgtc acgctcaggg atgtgatcag cattcaggat gataccttca ccggcctgag
     7801 tgttcaggta gtcgatagct gtatccaggc gatcgcgacg gggccagtat ttgctggcgc
     7861 gcttaacaat ggcttttctg gccatttcgt cggggaagct atcccacggg ctacttcctc
     7921 cgttatttcc ggctttgctg cacgccctga tgacctcgat ttccctgttg ctcatctctt
     7981 cggtcagata gtcgccttcg gatgttttta cgacacaata accgccaatg cgtgcgcccc
     8041 tgtctacgaa tgggttgtat ttgtgcgtgg gggcgcagtc aataccgttg gactcgtaaa
     8101 tgtccttctc gtaaacaagt ttgcattgcc cccactgaat ggctcctgtg acctgtgcca
     8161 gatgcagaag ccccatataa ctgatatcca ggcatacagc cttttttcgt gggaccagat
     8221 acgccagctt gctggccggg ttcagggtta tgccgatggc ggcaacattg atgatagcgt
     8281 tctgtgcgct gggcagattt gcccttgctg tgtcagccag aaaggcattt ttctggaatt
     8341 gctgaattgc aaactgactt tccttcgccc atgtcagcgt cggttcagtt aatgcctcgc
     8401 agaaaaagcg ctcctgctgc ttaacaaatt caacgatatc gaacattttt tggtcctgaa
     8461 aatcagaaag gacaggggga gaattttctc tcccattctt cttccgcccg agcataggcg
     8521 atcgctgaga tataatcgtt gtacgcctct tcagcttttt cgccagtgag tgctagttgg
     8581 gcttctttgg gtaaaaaaag gctgctcata agcaatggtt tatcggggaa catgctgata
     8641 agctcctgcg cccgatcatc aatccattta tccttttcat cctgaatttg ctgattaatc
     8701 cagcgacgct cctctatgcg gtcgcaggtg aggtatgcgt tcatggcgga actcctgatt
     8761 ccggttaatg cattaaatta atttgtcggg aaagctgaca tacagggcag ttacattctt
     8821 cctcctgctc cttagcgaag aaatatgcag cggcctgtaa tgcgatgtct tctggatgtt
     8881 ccgcgataaa cataacattg ccttccgtat caataacaga aatagcctca tcagacagga
     8941 tgacaaaata ggcgatgatt ttatcatcca taaaaacttc tcccattatc gttcctgctg
     9001 gagttacgac gctttttaca ttgatattta ttttttgatt gagcatgata tttcctttca
     9061 ggctggtgag attaacggtt ggcctttatt gttcaggtaa acttctattg catctgagat
     9121 aatgcgaatt ttttcaatca gtgaatgtgc gtaaagtgca ttattaacgt tcgctgacgc
     9181 catgtaataa cgcccgttgt aaagaattgc tgtgccgggt ttaacgtcct cgcgagaaac
     9241 taatgcggtt ccgtagtgag gtttgagcat gacaaatcct ccggttaatt aatccagata
     9301 ttttatttaa tccccgatat gtgatcgggg atggggttaa ttaaagatta acgttgaaac
     9361 caaagcggga agatttttcc gatgtgcggg aaatatccag caatttacgg cgcatttctt
     9421 ccgtcagatg cttgaaggcg ttaaattcgg cgacagcggc gtcaacgtta tagcctttgc
     9481 tgtgtagctc attgaggatg cgcatagttg ggcttggcat atcgaagagc acggaagcat
     9541 caaggctgat aaccttgcga tcaacatagt tcatcgtggc gtagggatgg ttatctgaga
     9601 accacgagag agggtagttg atagatagcg ttgattctgg aagctgaagt tgttctggga
     9661 ttttttgact gaaatagcag tcttccagtt tttcgaacac ttcccacgcc cgatcggttt
     9721 cgagcatttt tgcgtgacgg gctgcgccgc gttctgtcca gaggatgagg gagcgaacgt
     9781 tacgggcgat tttcacagaa ggtcttaaag acatgctgtg cttaaattcc cgcaactcct
     9841 cattctcaag taaaaagtag tgcttaccga taacaaatcg tccagaattc cgattgaaat
     9901 tttttcggat gtaatctggt tctgtgccat ataagctggc aagaagctct gtagtcacca
     9961 caggtatatt actaaatgaa attatggaga gggactccac ggagatggtt ttcatgcttt
    10021 gcgtcctact tgatattttg atttacccct tttgagaggg tgaccgggcg ctcaaaaccg
    10081 caagtagtcg gcgggcatat tcccattgct ggtattgtat tagccgcacg cccggtcata
    10141 aaccaagaag actggacaca aaaaaaccgc atgtctgtcg ggcgcggtaa ccgctacttg
    10201 ctaaggtgtt ttgagcacca tgaggcagaa tatatccccg ttaatgcagc attgtcaaac
    10261 gatctgttct ttaaagactt aacgagaagc cttttcggga atttcgatct gacagtcttg
    10321 aaatatcctg tagcgtgcgg cgcatttctt ccgtcagatg cttgaaggcg ttaaattcgg
    10381 cgacagcggc gtcaacgtta tagcctttgc tgtgtagctc attgaggatg cgcatagttg
    10441 ggcttggcat atcgaagagc acggaagcat caaggctgat aaccttgcga tcaacatagt
    10501 tcatcgtggc gtaggggtga tgctctgaaa accacgacaa agggaaattg atattcatcg
    10561 caggagaaga cagggccagt tgtttctgtt cccatagttg tttttccatg cgatcgaatt
    10621 cagcaatgta ggcttccttg aatgcagctg cttttttgcc ggtgaagccc atcaccagaa
    10681 aaacgaagcc gtttttggtg atttggtaca ttgggcgttg ttcgcctttg gcgtcggtgt
    10741 aggtaacgtc ctcaaaattg agggcgttaa atttctctga gcattcaata tttgcaatgg
    10801 cgcgcaatac gttatcgtgt cgcttgcaaa aatattcggc aacggcaaag gacgtagtaa
    10861 cagcgcgacc attggaaatg gtgatttcag gttgagaaag gactgggata gtagccataa
    10921 tggcagcctc cttgattggt gattgataac caccgctgga ggtgagaagc tcgctggcgg
    10981 tggactgtac agggttctca caactggcaa tcaagggaac cagcccgacc gaagtcggcc
    11041 ccatacagcc caccattgat aagatgtgcg tgtatgttga tacaaaaaaa gacgctggcg
    11101 cgtccgtatc gcctcgattg tcagcggggt gagaatcccg acacccgttt tatgaggtgc
    11161 tcgccaaata tagccccgac atcacacgca gtcaataccg tcctttctcg gaaatgcttt
    11221 ggcggtgccg cctgcacccc aaaacattcc ctgtattggt cagcgccaac ttcctgccag
    11281 tgttgcccgt tctcacgccg ttctcgctct cgcgcgggga tactctctca tcgaccggat
    11341 cgcacccgat gatacagcac gtttacgtgt aggggtctaa acaggtcatt gacgctgtaa
    11401 agctccagat tgttaaagag cattttgcgg cgggttaagt cgcgccgtac gactgattta
    11461 tgtagccctg tgtaagggcg cgatgtttct ggcttgaaat gaatataact tgcggtgatt
    11521 tttatgtaaa taccgttggt gctttttaat ggcgagaaat tattacttaa ctgattttta
    11581 aagtgattta ttttttaagg gggcagataa caggagggga tatgcaaaga aaaacccggc
    11641 gcatgggccg ggctatttat ctggtcctgt tctcattcag tagagtaggg gccaagaaat
    11701 cgcactccgt tgaagtgaat aagatgagaa ggtgcatcag ccacccatac ctctgtttcc
    11761 catgcgattt caccaagata tcgccccatg atggagcgat ttggaaaagc ggtcacatag
    11821 acaagtccgg ctgttgatcc ggcaaacagc ctggcaagct cagcatgccg cttcccatca
    11881 accggtccat gactggtgac agactcaacc agtagcagcc agtttttcgc agtaaaatgt
    11941 agcaccacat ctggcatttt accgtgtgaa tccacatcaa cacctagccc ggccagcagt
    12001 ggagcgtcga agtagcccca cttttcgcca gtgtcgccag catagaccag cacgctaccc
    12061 ggagcaaaac gtggggcgaa gtcctctatt atggcacgga taagttcgct gtgctcgccg
    12121 ggactgaggg ttatttgctg gcccgcagca atttcaacag ggatacgatt ctgttcgcgc
    12181 tccttagcat agcgggtaac cagcgtttca cgctcggcta aataggttgc aaggctatcg
    12241 tgccatgccg ggctgccgaa agtgcgtagc atggacagag cagcaggttc gatctgataa
    12301 acagccttcg ggctgttcac tgggcgatcg ggcttgtccg gattgtagag ggctactcca
    12361 gcagcgcaga actgatgcat agactggcgg cggaatgtct cacgagtatt gggtgcgtag
    12421 accttgccgt agtgctcccg aacccaattc atgattggtg taatgcccac aagcggattt
    12481 tccgcgtcgg cccatgcttt tcccggtgtg aggttcagga gtgccagcaa acacaaagca
    12541 gaacgctcat tctgctgcgc tctgggcagg cctaaggatg ctatgatttg ctgagcggcc
    12601 tcaatataat cattttggtt gttcatgcag tcagagctcc tagcttagcg tcaatatgtt
    12661 cttgcgtgag agtttcttgt tgcatagccc acttgccaag ctctatcaga gtgtcacggc
    12721 ttgggtactt aatcatcttg aggtcggttg cattgacctg tgtatgcccg ttgaagcgac
    12781 ggaaatactt atccactgcg gtagtgttca ggaacacggc caagccacgg gccagcgcct
    12841 cgggtaaccc atgtttgttc tcatgaaaca cgttcatgtg attttcaaat cccaacactg
    12901 aatactcact gaaggtagca ggatcaacga cgcttgctac cactcggcgc ttttcctcct
    12961 tcgacgagaa acgacgcacc acacaataaa aaccgttcgg gtaaagccac ttttctgtct
    13021 cgtcgttgcg catgattgcg ttgggtttct tcaaaccttc cacaggccag acagtgctac
    13081 tcatactcaa atggctcggg tagatcaggg gaacagttcc ttcttctggc atgctgcgca
    13141 aatgagcttt cagtcggaaa tcaactatcg gtccggtcga taccttcacg ccaatatcag
    13201 ccagcgaaca ttgtacggca gatgataact caatggcgct tttctcgagc gtggtcggca
    13261 catgaataaa ccgctccgaa tcgtccgggt acacaatctg gtcaaatggg tgttcgtagg
    13321 tgaccaggtc aaaaaagcta tcatcggtcg aagttgtgac tgtaacagcc tcctgacggc
    13381 ctccacgctc caggcggatg ataatatttt cctgcaacac atcgtcatcc ctgaaagctt
    13441 tgctacgtga ttcgaacaaa tgaatatggc ggattgctgc ccgctcaagt atgaaattac
    13501 ggaacggacg gtaatacggt ccgttgcaga aactacgtgg gattatggcc actatctgcc
    13561 cgccaggtgc aacctcacct acagccagtg caacaaaggc ggaatacaga tttaccgtct
    13621 cgataccgac agtgcgaaga gcctgccggt gggctgactg gctattgatc ttcttgtaag
    13681 gcggattaag tattgcatgg gtataacctc gatcctgtag accttcggcg gttgccagct
    13741 caatataatc cccttcgatg atgcggggcg tcactctcct gtacccggtt aagtgcttcg
    13801 caaggtgacc acacagcttt ttgtcgattt cgtaggctgt tgcctcgaca gactcaaagc
    13861 caaactcgcc aaccagccaa cggtcgagga aggcgcaaga cagtgcgcct acaccagcgc
    13921 ctgcgtccag tagacggcaa gttttcattg tgctcggtgg aaacaaggag gccataaaac
    13981 gggccacgcc agaaggcgtc atgaactggc cgaactcagc tttgtgttta cgcgcggtac
    14041 ggggagacag ctcgcgacgc acgctgtctg cgacgtctag cttttgaagc ataactaatc
    14101 ctctttacgt ggcatgatga tgccaacgtg cttttgacat cctccacatt cttcaggcgt
    14161 gaaggatgtc aaatcactct gtgagctcaa tcaaaagtct catcattcca ctgcgatttg
    14221 atgacctttc cgacaaactg gcagttttcg ttacactcaa tcatgtcgaa tcgtgggttg
    14281 agcggctcca ggtacgcttt accgctctca cgaatgaatc gtttgaaggt aaattcatcg
    14341 ccgttcattc gggccacgca aaaatcgcca tcctcgattt cccgttcagg gtcaacgagt
    14401 atcagcatac cttcagggaa gctgggttta ccaccctgcg gtgcggtcat tgaatgcccc
    14461 ttgacctcca gccagaacga cctgtcactg gcttttgtgg tggtggagat ccatttaatc
    14521 gcatcttttg cggtgtacga aatatcatcc attgagaatg cgccagcttg tacgcaggag
    14581 agtaacggat attcgtaagc ggggacacga gttaatggct cttcaattgc gagcgccatt
    14641 tctgcaatag attttgccag gctgggactg aaatctccga cctttacatt cagtatctta
    14701 gcaagctgtg ctgcgtggct ggcatttatg gcgttgatgc cagccattag ctgattaaca
    14761 gcgctttggg taacacctaa ttcataggct aatgattcct gcgacaggcc taactctttc
    14821 tttttagcgt taaaaatact tttcagacgc ttggcgtctt ccagttgctc aggggttaac
    14881 ggtttctttt tcatgttcat accatatcac ctttggttat atgtaatgaa atatctgcgg
    14941 tgttgacatt ttaataactg tgcgtaatga tgtgggcatt ttgaaggagt cagttatggc
    15001 gatgaaaaaa gagaccctgg ctgattacgt atacaggttc ggtcagaaga aagccgctaa
    15061 ggactttggt gtggcgcaga gcgcgatcag taaagcgctt ctcgtcggac gtgaaattta
    15121 cgtcaaaacc tttgatgacg gcagagttga ggcggaagag gttcgaccgt tccctgcgtt
    15181 cgtaagaggc gatgattaat cctggacaca cagcccatcg gaggtgtgag tgaacaggca
    15241 aatcaaaacg gttatgcccg agcagtactg tgatgctgat cgggactgga tacaggagca
    15301 attgttacag ctagacccga ccacccgggt aaaaattgca gcagagtacg cgagagtgta
    15361 ccaggaagag tgggacaagg agcctgtatc gttcaggaag ggcaacaggg caagacggag
    15421 cgccaacacc cggttacggg tgtatgtcca gaagtacgcg agagccagcc gtggctggat
    15481 gctttcgcca gtagcggtac ggaagtttcc cccgacacca tgatggcggt tagttgcgct
    15541 agagataatt tcacatgcaa ctaatttcct gaaaggaagc gaggaggggg taagggggtg
    15601 gagtcgttgg taaggcctgt atcccttggc ctaggccaga aggcaggtac ataggttagg
    15661 tagatcactg tataagcgag cgatagtttc tggtgaaatg attatcgagc taactatttg
    15721 gtgataaact atgtcagcag caaccaaatc ggcactgatc cgtacactga gcactgtacc
    15781 actgagaaca gaggagcgtt acagctttct ggctgatgtt gtaacgctcc ttgagtcaca
    15841 ggggatgcat gtggcaagca atgtcacagt aagaatcgat ggcagaaatt tccgtgttga
    15901 tattctcgca acagcaaaaa ctggtgggag tgtggctatc gagatcgatc gtagctctcc
    15961 gcgatcccgc tcagtcatga aattgcgtga attggcacgt cgtggcactg aaggctttgt
    16021 gttgcttcga atgccaaaga aactgaccag ttatagcgat gctgggatcg atatcatccc
    16081 ggcgaatggc aagggggcgt catgctgaaa atcaccccaa actttgctca ggagcgcggt
    16141 ttaaaccagc tgcggcacca gtggaaacag catcgcacgt acctgatgta tgcacccact
    16201 ggctctggca aaactggtct tgcagcattc gtcactgccg gaatggttga acgtggtatg
    16261 cgggtgatgt ttgtctgtcc gtacaccatc ctgctgaacc agacagcgga acgtttcacc
    16321 gaatacggtt tgccgtggga agaaatcagt tttgtatggc gtgatcaccc taactatgac
    16381 ccgtcacgac tgattcagat tgccagtgcc gacacgctga tccgtcgtga gtttcctgac
    16441 aacatcgatc tgctgatcat cgatgaagca cacatgaagc gtcgtgcgtt acttgaggtt
    16501 atccgggaca aagatatcag ggttcttggg ctttccggca ccccgttcgc agcctggatg
    16561 gggaagtact acgaatgcct gatcaagcct acaacgattc gtgaacttat tcagcgtggt
    16621 gatctgagcg attatgagtt ttttgccccc tcaatgcctg atctggctgg cgtaaaaacc
    16681 agtaacaccg tatttggacg ggattacaac gaggaacagc tcgcatccat catggggagt
    16741 tcggatctgg ttggcgatat cgtcagcaac tggcttgaga atggcgagga tttaccgacg
    16801 atctgcttct gcgtgaatgt ggctcatgcg aactttgtta cccgtgagtt tttacagtct
    16861 ggcattggcg cagaggtgat gacggcagat accccccatg atgaacgaca ggacatcatt
    16921 cgccgttttg aagagggtgc gaccaaaata attgtcaacg ttggcgtact ggttgcggga
    16981 tttgacagcg atgttcgctg cctgatctac gcccgtccga ccaaatcaga aatccgctgg
    17041 ttgcagtgca tcgggagagc gttacgtact gcgtctggta aaaaacgcgc attgattttc
    17101 gaccattccg gcacggttca tcggctgggc ttccccgagg atatcgagta tgacgaactt
    17161 ccgggaaaaa atgacggaat gaaagcgtct gctggcggga gtgaggttaa ggctgagaaa
    17221 cttcccaggg aatgccccaa atgccacttc atgaagcctg ctggcgtgca tatgtgtccc
    17281 aaatgtggat ttcatcctct tggtggtgat gacgtggcga cagatcgcga tcgtaagctt
    17341 tcacgcgtca acaaagggaa acgcgaatac acccgtgagg aaaaacagcg ttggtggagc
    17401 gagatcaagg ggtatcagaa ttaccgcaac gcgacaggta agccactgag tgacggatgg
    17461 tgtgctcata cctacaagga gaagttcggg gtttggccta aaggcttcag taatgcgccg
    17521 ctgcaaacct cagttgaagt gtacaacttc atcaagtcaa agaccattgc ctatgccaaa
    17581 gggcgcaaga aagccatgac aggaggccag catgcgaact agagatgcag ccgtggggca
    17641 ctggagccgg atttttgaat attacggcat gcctcctgtt actggtgtga aacattacaa
    17701 cggaccctgc ccgatatgtg gtgccagagg taaatttcgc tgtgatgata aggatggttc
    17761 cggttcatgg atttgcgtct gtggtcacgg ggacgggatg aatctgttgc aacttgccac
    17821 gggtaagccc tgggtgacgt tgtgtgatga aatcgatcgg ctgatcggaa atacctggaa
    17881 gagggagaaa gtcagccagc ctgtaacaga gataagcaga aagcgggagc tggtcataga
    17941 taagtttgcc ggactcccgt gtctgcgggg tacaacaggt gaggcgtacc tgcaggggag
    18001 gggaatactc cagttaccga ccgaatccgt gcgtttttgt gaccgtcaga tcgccagcgg
    18061 gcgcgaatat caggcaattt acgccattgc aacagatgac aaaggttctc tttgctatct
    18121 gcatcgtacg ttgctggatg gtgatcgcaa ggcgaatgta gaggcggcta aaaaaatgac
    18181 cgcgctacag gagttgcctg gtttgcagca tgccaaatcg gtggcaatac gcctgtatcc
    18241 ggtgtcgtcc actctgggga tagccgaggg tatcgaaact gcgctttcat gccgtcaaat
    18301 cttccgctgc aatgtgtggt caacaatgaa ctccggtttt atggagaagt tcattgcgcc
    18361 acctggcgtt aatcacctga ttatctttgc tgacaatgat gcgcacggcg caggtctggc
    18421 ggctgccttt aaatgtgggc ataagaatct catgagttgc aatgacgttg agaaagtcag
    18481 cattcgctgg cctgacttgc cggattttaa cgacatgctc attcaggggt gtgaagcccg
    18541 tgaacatgtg ttgacgcgca aattcaaagc ggaggctgcc tgatggaaat agagatgatc
    18601 aaggcggcta atggcgtatt tgtaccggcg tatgagcgcg atttaccccg actggcaaaa
    18661 tttaaaaacg gtgagctgta tacactggaa gcaaaactta cccgtaaccc atcttttcac
    18721 cggaagatgt tcgcttttct taatttctgc tttcagtact ggtgcgctga acatgctgga
    18781 tacgaattct ctgatgaagc gacgcagttt gatgagtttc gtaaaaatct gacaattctt
    18841 gccgggttct atgatgtggt cacaaccata agaggcgagg tgagatatcg ggcaaaaagc
    18901 ctgagttacg cgaatatgga tcaggatgag tttgaacgtt gctacaacgc aatgattaat
    18961 gccgcgttaa aacatgtgtt cgggcgctca aacaatcctg aactgaataa ccgcctgctg
    19021 tcgtttttct gaggtgatga tgaagcaacg taaacccaaa aaatgcaaag tgtgcggctc
    19081 ctcgtttgtg ccgttccgct catatcagaa agtttgctgt ggtcagtgcg cacttgaact
    19141 ggtcagaaaa gaaaaggcca tagcctctgc aaaagaacag gcagacaaac ttaaagcgcg
    19201 aagaagggac ttacagcccc gcagttactg gattaagcag gcacagcagg ccgtgaatgc
    19261 ttatatcagg gaacgagacc gtaatttgcc gtgcgtttca tgcgggacat tcgattctgc
    19321 tcagtgggat gcaggtcatt accgtacaac agctgcggct ccccaactca gatttgatga
    19381 acgcaatatc cacaagcaat gcgtggtgtg taaccagtac aaaagcggaa atctcgttcc
    19441 gtatcgcgtc gaactgatta accgcatcgg gcaggaagca gtagacgaaa tcgaatcgaa
    19501 ccataaccgc catcgctgga ctgtcgaaga atgcagggcg atcaaggcga agtatcagca
    19561 gaaacttaaa gacctgcgaa acagcagaag tgaggccgca tgacgttcac agtaaaaacc
    19621 atttctgaca tgctcgttga ggcatatgaa aatcagaccg aggtagccag aatactgaac
    19681 tgtagtcgca acacggtcag aaaatacact ggcgataaag aaggaaaaag acacgctatt
    19741 gtcaacggtg ttcttatggt tcatcgcgga tggggtaaag atactgatgc gtgatatccg
    19801 gcaggttctt gagcgctggg gtgcatgggc ggcaaataac tatgaggatg ttacatggtc
    19861 gcccattgct gccggattta agggactgat ccccgaaaaa gtaaaatcac gtccacagtg
    19921 ttgtgacgat gacgcgatga ttatatgcgg gtgtatggct cgccttaaca ggaacaacag
    19981 tgatctgcat gacttgctgg ttgattatta cgtgttgggg gagacgttca tggcgctggc
    20041 acggaaacat gggtgctctg acacctgtat aggtaaacgc cttcacaaag cggaggggat
    20101 tgttgaaggc atgctgatga tgctgggagt gaggcttgag atggatcggt atgttgagcg
    20161 tgaattgccg ggagggagaa cctctgtatt ttatcagcga aaaaatagtt tacgatcgta
    20221 aaaatctgca tatcatgata agagtggtta cattgccacg ctgcttaacc cgccgatgcg
    20281 cgggtttttt gtatccagaa tcctgttagt tatacggaaa gtacacagaa aggaaggtgc
    20341 gaccgcaatt aataacaaaa tcttaaaaat cgcatatggc actattagtt ttctaaatat
    20401 tgtatatttt aagtattgca ggataaccct gtaacgaagt ttgcgtaaca gcattttgct
    20461 ctacgagttt gccagcctcc cccagtggct ggctttttta tgtccgtagc gtcaaagcag
    20521 caatgtcgct ggggcgtcgt gcaattggcg ttgagctgga gagcgggcgt tttgagcaga
    20581 cggtcaggga agttcagaat gtagtcagtc agaacggatg atattgcaga attagttacg
    20641 taccgttatt atcctgcgcc cggcccttta gctcagtggt gagagcgagc gactcataat
    20701 cgccaggtcg ctggttcaaa tccagcaagg gccaccatca cataccgcca ttagctcatc
    20761 gggatagagc gtcagccttc gaagctggtt gcgcggggtt cgagtcctcg atggcggtcc
    20821 attatctgca cactgcgttg ttagctcagc cggacagagc aattgccttc taagcaatcg
    20881 gtcactggtt cgaatccagt acaacgcgcc atatttattt accaggctcg cttttgcggg
    20941 ctttttatta caggaaagac actggacagt gaaatgttaa atgcctcaca attcaggcag
    21001 ttgatgattg tctggttgac ggagagttgt taaaaaagtt ttgcatggtg aatccccctg
    21061 agcggagggg cgactggtga cggtataatc tctgattatc aaaacgagaa tgacgagggt
    21121 ttagtggcac cgggctgaac tcaccgggag gcacccggca ccatgcaatg gcacatagcg
    21181 ccactctcca gcccctctcc ggaggggctt tcttatggac aaaaaagccc gcgctgggag
    21241 acgcgggcgg caaggaataa acaatgaaac gtgaagtaat atttcagctg gcgaataata
    21301 ccctatagta atcactctgc gcaactgcgc gacctttttc gaattgcggg ctgtagtctc
    21361 ccttctgcca ttgtcctgta acttccggac ttcagcccgc tccttatttt actcacaata
    21421 ttatcccggc cgggaggatt catggcattt aaacactatg atgttgtcag ggcggcgtcg
    21481 ccgtcagacc ttgcgaaacg aataactcaa aaactgaagg aagggtggca gccttatggt
    21541 agtgcgctga tttcgacagc tggttatggt gcggagttca tccagccagt tgtgagtgag
    21601 gggagcatct catcaccaga ggagccaggc aaccgtccga cgacctcagc gccttctgtt
    21661 gcgccagaat attactatgt gatcgcgctt gctggtcagt ccaatggtat gtcatacggt
    21721 gagggactgc cattgccgga tacattcgac agccctgatc cacgtattaa acagttagcg
    21781 cgtcgcagta cggtgacacc gggcggtgca gtatgcaaat ataacgacat cattccggcg
    21841 gaccattgtc tgcatgatgt gcaggacatg agccgtctta accatccgaa agcggacctg
    21901 tcaaaggggc agtacggaac cgtggggcag gggctgcata tcgccaaaaa actgctgccg
    21961 tttataccgg cgaatgcggg cattctgctg gttccgtgct gtcgtggtgg ttcagcgttc
    22021 accaccggag ccgatggcac atacagtgac gcgagtggtg cctcggagaa ttcaacccgc
    22081 tggggtgtgg acaagccgct gtataaggac cttatcggtc gaacaaaagc agcactgaag
    22141 aagaatccga aaaatgtgct gtttgccgtg gtgtggatgc agggggaatt tgattttggc
    22201 ggtacgccgg taaatcacgc cgcacagttt ggtgcgctgg ttgataaatt ccgtgcagac
    22261 ctggcggata tggcaggcca gtgcgtcggt ggctctgctg gcggtgttcc ctggatatgc
    22321 ggggacacga cgtatttctg gaagcagaag aacgaatcca cgtaccagac ggtgtatggc
    22381 agctataaaa acaaaacgga aaagaatatc catttcgtac cgttcatgac ggatgagaac
    22441 ggggtgaatg tgccgacgaa caaaccggaa gaagacccgg acattccggg tatcggatat
    22501 tacggttcga aatggcgtga cagctcagcc acctggacgt cacaggacag ggcgagccat
    22561 ttcagttcat gggctcgccg cgggattatt tccgaccgtc tggcaacggc gattttgcgc
    22621 catgcgggaa gagtggcgct aaacgcgggg gcatcatcga cagtatcaga ggtgcgcccg
    22681 tcatcgcctt ccggtgcaga agccacaggc gtcacaacac tgctctctta ccttgccagc
    22741 gagtcagagg gaagcctgaa agtacaggga tggtcagcca gtggcggcag ggcagaagtg
    22801 gtcagcgatg cggagggaac cggaggtaag gcagtgaagc tgaccaagga agccggtaaa
    22861 agcagctggg tgctggagta cgccgcgggc aacggtgcgg ctctgttaca gaaagggggg
    22921 cagattcgct
//
LOCUS       DAZUFB010000072        22725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZUFB010000072 DAZUFB010000000
VERSION     DAZUFB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.072"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..432)
                     /locus_tag="ACHY3M_003194"
     CDS             complement(<1..432)
                     /locus_tag="ACHY3M_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4318056.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLL"
     gene            complement(442..993)
                     /gene="ybcL"
                     /locus_tag="ACHY3M_003195"
     CDS             complement(442..993)
                     /gene="ybcL"
                     /locus_tag="ACHY3M_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFS4318057.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEMKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1162..1494)
                     /gene="emrE"
                     /locus_tag="ACHY3M_003196"
     CDS             complement(1162..1494)
                     /gene="emrE"
                     /locus_tag="ACHY3M_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFS4318058.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1828..2142)
                     /gene="fliE"
                     /locus_tag="ACHY3M_003197"
     CDS             complement(1828..2142)
                     /gene="fliE"
                     /locus_tag="ACHY3M_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS4318059.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2356..4014
                     /gene="fliF"
                     /locus_tag="ACHY3M_003198"
     CDS             2356..4014
                     /gene="fliF"
                     /locus_tag="ACHY3M_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS4318060.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRVSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFREQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PVPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAHEEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4007..5002
                     /gene="fliG"
                     /locus_tag="ACHY3M_003199"
     CDS             4007..5002
                     /gene="fliG"
                     /locus_tag="ACHY3M_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS4318061.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4995..5681
                     /gene="fliH"
                     /locus_tag="ACHY3M_003200"
     CDS             4995..5681
                     /gene="fliH"
                     /locus_tag="ACHY3M_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS4318062.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5681..7054
                     /gene="fliI"
                     /locus_tag="ACHY3M_003201"
     CDS             5681..7054
                     /gene="fliI"
                     /locus_tag="ACHY3M_003201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS4318063.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTGS"
     gene            7073..7516
                     /gene="fliJ"
                     /locus_tag="ACHY3M_003202"
     CDS             7073..7516
                     /gene="fliJ"
                     /locus_tag="ACHY3M_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS4318064.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7513..8640
                     /gene="fliK"
                     /locus_tag="ACHY3M_003203"
     CDS             7513..8640
                     /gene="fliK"
                     /locus_tag="ACHY3M_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS4318065.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISSESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8745..9209
                     /gene="fliL"
                     /locus_tag="ACHY3M_003204"
     CDS             8745..9209
                     /gene="fliL"
                     /locus_tag="ACHY3M_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFS4318066.1"
                     /translation="MTDYAISKKSKRSLWIQILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9214..10218
                     /gene="fliM"
                     /locus_tag="ACHY3M_003205"
     CDS             9214..10218
                     /gene="fliM"
                     /locus_tag="ACHY3M_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFS4318067.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10215..10628
                     /gene="fliN"
                     /locus_tag="ACHY3M_003206"
     CDS             10215..10628
                     /gene="fliN"
                     /locus_tag="ACHY3M_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFS4318068.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10631..10996
                     /gene="fliO"
                     /locus_tag="ACHY3M_003207"
     CDS             10631..10996
                     /gene="fliO"
                     /locus_tag="ACHY3M_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFS4318069.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10996..11733
                     /gene="fliP"
                     /locus_tag="ACHY3M_003208"
     CDS             10996..11733
                     /gene="fliP"
                     /locus_tag="ACHY3M_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS4318070.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11743..12012
                     /gene="fliQ"
                     /locus_tag="ACHY3M_003209"
     CDS             11743..12012
                     /gene="fliQ"
                     /locus_tag="ACHY3M_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFS4318071.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12020..12805
                     /gene="fliR"
                     /locus_tag="ACHY3M_003210"
     CDS             12020..12805
                     /gene="fliR"
                     /locus_tag="ACHY3M_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS4318072.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13095..13718
                     /gene="rcsA"
                     /locus_tag="ACHY3M_003211"
     CDS             13095..13718
                     /gene="rcsA"
                     /locus_tag="ACHY3M_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFS4318073.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13762..13950)
                     /gene="dsrB"
                     /locus_tag="ACHY3M_003212"
     CDS             complement(13762..13950)
                     /gene="dsrB"
                     /locus_tag="ACHY3M_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFS4318074.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14113..14340
                     /gene="yodD"
                     /locus_tag="ACHY3M_003213"
     CDS             14113..14340
                     /gene="yodD"
                     /locus_tag="ACHY3M_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFS4318075.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14638..15453
                     /gene="yedP"
                     /locus_tag="ACHY3M_003214"
     CDS             14638..15453
                     /gene="yedP"
                     /locus_tag="ACHY3M_003214"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFS4318076.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15450..17144)
                     /gene="dgcQ"
                     /locus_tag="ACHY3M_003215"
     CDS             complement(15450..17144)
                     /gene="dgcQ"
                     /locus_tag="ACHY3M_003215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFS4318077.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17315..17497)
                     /gene="yodC"
                     /locus_tag="ACHY3M_003216"
     CDS             complement(17315..17497)
                     /gene="yodC"
                     /locus_tag="ACHY3M_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFS4318078.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVESRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17576..18493)
                     /gene="yedI"
                     /locus_tag="ACHY3M_003217"
     CDS             complement(17576..18493)
                     /gene="yedI"
                     /locus_tag="ACHY3M_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFS4318079.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18666..19586
                     /gene="yedA"
                     /locus_tag="ACHY3M_003218"
     CDS             18666..19586
                     /gene="yedA"
                     /locus_tag="ACHY3M_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFS4318080.1"
                     /translation="MRFGQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19575..20045)
                     /gene="vsr"
                     /locus_tag="ACHY3M_003219"
     CDS             complement(19575..20045)
                     /gene="vsr"
                     /locus_tag="ACHY3M_003219"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFS4318081.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20026..21444)
                     /gene="dcm"
                     /locus_tag="ACHY3M_003220"
     CDS             complement(20026..21444)
                     /gene="dcm"
                     /locus_tag="ACHY3M_003220"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFS4318082.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21511..22206)
                     /gene="yedJ"
                     /locus_tag="ACHY3M_003221"
     CDS             complement(21511..22206)
                     /gene="yedJ"
                     /locus_tag="ACHY3M_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFS4318083.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22246..22527)
                     /gene="drpB"
                     /locus_tag="ACHY3M_003222"
     CDS             complement(22246..22527)
                     /gene="drpB"
                     /locus_tag="ACHY3M_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFS4318084.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5575 a         5964 c         5771 g         5415 t
ORIGIN      
        1 aagtagaacg cccattgcta agtgttcctg aatttgcgtt gagaagcctc gggaaataga
       61 tgtttttagt tgctcccaac aatatattaa ctcgggattc tgaggtaagc agaaacctgt
      121 cgtgttacga atttgatcgg ttatcgcata aagattttgg aacttttcaa ttagatcaat
      181 gggataataa agcatttctg caagataaag acctgcttct gggtaattcg caatataaaa
      241 ttcatatcca cagggaaata atattatctg attattatca acagttagac tatgcgtctc
      301 ccaattgata actttttttc cctgacggat acgacataaa gctggcataa gaggcttaac
      361 cctgtgaatc tcatgatgtt tatgcatccg tatttcttca atctttaggt tagtcctatc
      421 ttttgccagc atactcttat cctactttac ctcataaacg ggtattatct cagcggttgc
      481 aattttattg gcattaagca tataaccaac taacgctccg ctggaattag aatccacagg
      541 aatcttatca gtttttagag cccatatttt aaactggtaa tgatggggtt tatctccttt
      601 gggaggacat gctccaccaa atcccgcata gccaaaatca tttcggcctt ggacagcacc
      661 agtcggaagt tttgtaccat cacgtcttcc tgcatcagcg ggcaaatatg ttaccgttgc
      721 tggaatatta gcaacagtcc agtgccacca tccgctgcct gtaggcgcat ctggatcata
      781 cacagttacg gcaaagcttt tggtgccttc tggagcacca gaccaggtta atgagggcga
      841 tgtattaccc ccctcacacc caaatccaga aaatacatga gacgttgtaa gttgctctcc
      901 tgttttcatt tcattactag tgacctgaaa tgctgcggcc tgcgcagaaa atgttatgaa
      961 tgccaataca cttgaaacga taagtttttt cataaaaacc tctttgttgt gacctatcgt
     1021 tattttattt gatgtgcatt gatctcatta tgcaaaaagg cgcaatgatc gcgcaaatgc
     1081 aatcataatt ataaagttgc cccgaacatt agccataata tgcacaaatc agattcctgt
     1141 tatctcaagg atgattattt tttaatgcgg tgcgcttcgt gacaacaagt taatgactaa
     1201 cacaccggca caaatcaaca tcatgcctat gatggctggc aagtccagtc gttggccaaa
     1261 aaatccccag gaaagcaaac taatcagaac gataccgact cctgaccaga tagcataagc
     1321 aatccctgta ggtatataag ccagtgtctg agctaataac caaaatgatg cacaatagca
     1381 aataattgtg ccaacagagg gccataaccg tgtaaacccc tctgaaaact tcattaaggt
     1441 tgtaccaatg acctctgcaa gtattgcgcc accaagataa atataagggt tcatagcata
     1501 ttctttcctg ttcatactgg agagaattgt actacagttt gaacacaact caccgttttc
     1561 atcctggtgt atgtgtatcc agggggctct ggcgtggcaa gagataaagg caggaacaat
     1621 aatgatcaga aggaacccca ggctaacaaa agagttcact cttcctatga gcaaagcgaa
     1681 gacaaaacat gttatcgcgt ttacggatcg tggatggcag aaaactttca ggatcaggta
     1741 tccattttaa accagaaata gggtgagttt gccccatcca tgccccacgc gggcagatgg
     1801 gatgtaatta ccaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc
     1861 cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgagg ctttttgcat
     1921 atcggtcatc acatcgttta acgccacgcc gggttcaccg agggtgaatt tttctgcctg
     1981 cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc gcggcgtgca gctgcccggc
     2041 aaaactaatg gtcggttgtg gcagtgattc ctgtgcacgc gcactcatcg ccgtagcctg
     2101 taactggctg ataacccctt caatcccctg tatcgctgac attttcgtct cccggataat
     2161 ttctggtagc aaagcctacc agtaagtcaa taagacaaag gcgctaaata gcaacaaaaa
     2221 aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taatgggagc gtcaattttt
     2281 cgagtttgct gacccgggag tgagtctgtt ccactttgcc aataacgccg tccataatca
     2341 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
     2401 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
     2461 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
     2521 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
     2581 gtcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga gctgcgtctg
     2641 cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact actcgatcag
     2701 gaaaagtttg gtatcagcca gttcagagaa caggtgaatt atcagcgggc gctggaaggc
     2761 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     2821 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     2881 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt acatctggtt
     2941 tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc tggtggatca gggtggacat
     3001 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc
     3061 agcgatgtcg aaggccggat tcagcggcgt attgaagcga tcctgtcgcc aattgttggt
     3121 aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg
     3181 gaagaacagt atcgccctaa cggtgatgaa tctcaggcgg cgcttcgttc acgccagctt
     3241 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     3301 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
     3361 cagcagcagg cgagcaccac cagcaatagc gggccgcgta gcacacagcg gaatgaaacc
     3421 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     3481 cgtctgtcag tcgcggttgt ggtgaattac aaaaccttgc cagatggcaa accggtgcct
     3541 ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct
     3601 gaaaaacgcg gtgactcgct caatgtcgtc aactcgccgt tcaatagcag tgacgaaagc
     3661 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttact tgctgccggt
     3721 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc gtaaagcggt acgcccgcag
     3781 ctaacacgtc gcgctgaggc gatgaaagct gtacagcaac aggcgcaggc ccacgaggaa
     3841 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactacaaca acggcgcgct
     3901 aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     3961 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     4021 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     4081 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
     4141 gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
     4201 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     4261 gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
     4321 cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga
     4381 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
     4441 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     4501 cggcgtgcag ccagccgcgc tggcggagct gaccgaagta ctgaatggct tgctcgacgg
     4561 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     4621 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg acggcgagct
     4681 ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgacgatcg
     4741 cagcattcag cgtctgttgc aggaagtaga ttccgaatcg ctgttgatcg cgctgaaagg
     4801 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     4861 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     4921 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
     4981 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg
     5041 cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg
     5101 ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
     5161 atcaggcggg gattgccgaa ggtcgccagc aaggtcatga gcagggctat caggaaggac
     5221 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
     5281 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcactt gatagtgtga
     5341 ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtcatc ggtcagacgc
     5401 caacagtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     5461 tattcagcgg taaaccacag ctgcgcgtgc acccggatga tctgcaacgt gtggatgata
     5521 tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg gggcgatccc acactccatc
     5581 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     5641 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     5701 ctggctaacc acgctggata acttcgaagc caaaatggcg cagttgcctg cggtacgtcg
     5761 ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     5821 gctcggcgca acctgtgtca ttgagcgtca gaacggcagc gaaacgcacg aagtagaaag
     5881 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg
     5941 tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg gctgaaggac tgcaaagcgg
     6001 caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt ctggacggca gcggtaaacc
     6061 gctcgatggc ctcccctccc ccgatacgac ggaaaccggt gcgctgatta ccccaccatt
     6121 taacccattg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     6181 tgccctgctt accgtcgggc gcgggcagcg tatggggctg tttgccgggt ccggcgttgg
     6241 taaaagtgtg ctgctgggga tgatggcccg ttacacccgc gccgatgtca ttgtcgttgg
     6301 tttaattggt gaacgcggac gcgaagtaaa agattttatt gagaatatcc tcggtgccga
     6361 aggacgtgca cgctcagtag tgattgccgc tccggcggat gtttctccgc tcctgcgaat
     6421 gcagggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
     6481 gttgctgatt atggactccc tcacccgcta tgcgatggcc cagcgtgaaa ttgcgctggc
     6541 gattggcgaa ccacccgcca ccaaaggtta tcccccgtcg gtgtttgcca aattaccggc
     6601 actggtcgag cgtgccggaa atggcattag cggcggcggc tcgattaccg cgttttatac
     6661 cgtgctcacc gagggcgatg accagcagga ccccattgcc gactctgcgc gggcaatcct
     6721 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
     6781 tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
     6841 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
     6901 cggcgcgtat gccaaaggca gcgatccgat gctcgacaaa gccatcgccc tgtggccgca
     6961 gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca
     7021 ggggctggag cgtattttcc cgacagggtc ataacccagg agataacggc agatggcaga
     7081 acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
     7141 cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa gaacagctca aaatgctgat
     7201 cgattatcag aatgaatatc gcaataacct caacagcgat atgagtgccg ggatgaccag
     7261 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
     7321 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
     7381 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
     7441 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
     7501 gaggaaacct gaatgattcg cttagcgccc ttgattaccg ccgacgttga caccaccaca
     7561 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
     7621 ttagcaggcg agacaactac cgacaaagcg gccccccagt tgctggtggc aacagataag
     7681 cccacgacaa aaggcgagcc gctggtcagc gagattcttg ccgatgcgca acaagcggat
     7741 ttactgatcc ctgtggatga aacaccgcct gtcatcaacg acgaacaatc cacatcaaca
     7801 ccattaacca ccgctcaaac gatgacgttg gctgcggtgg ctggcaacaa tacggcaaaa
     7861 gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg
     7921 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg cgccgtcaac cgtgttaccg
     7981 acagagaaac caacgctctt tacaaaactg acttctgcgc aactcacaac agcacagcct
     8041 gatgatgccc ccggcacgcc agctcagcca ttaacaccgc tggtagcaga agcccagagt
     8101 aaagcggaag tcatcagcac accgtcaccg gtgaccgctg ccgccagccc gctaattact
     8161 ccacaccaga cacagccact gcccaccgtc gccgcgcctg tgttgagtgc accgctgggt
     8221 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa
     8281 caaagtgcag agttgcgtct gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa
     8341 gtggatgata accaggcaca aatccagatg gtttcaccgc atcagcatgt acgcgccgcc
     8401 ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg
     8461 caaagcaaca tcagtagcga aagctttagt ggtcagcagc aggccgcttc ccaacaacag
     8521 caaagccaac gcacagtaaa ccatgaacct ctggcggggg aagaagacga tacgcttccg
     8581 gttcccgtct ctttacaagg gcgcgtaaca ggcaacagcg gcgttgatat tttcgcctaa
     8641 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
     8701 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
     8761 taagcaagaa aagcaagcga tcgctttgga tccagattct ggtattcatt accctcgcgg
     8821 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gaagacaaag
     8881 cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatc
     8941 tgggcgatgc agatcgcgta ctttatatcg gcataaccct gcgcctgaaa gatgaagcta
     9001 cccgctcgcg gctgagtgag tatttgccgg aagtccgtag ccgtttgctg ttactgtttt
     9061 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
     9121 ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
     9181 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
     9241 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt
     9301 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
     9361 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
     9421 ttcaacctgc tgcgtcgtag cccggatata accgtcgggg ccatccgcat tcagccgtac
     9481 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
     9541 ctgcgcggca cagggctggt ggtgttttca ccgagtctgg tgtttatcgc cgtggataac
     9601 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatacc
     9661 gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg aaggctatag cgacgcctgg
     9721 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
     9781 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
     9841 aacctgaccg gcgaatttaa tatctgtctg ccattcagca tgattgagcc gctacgggaa
     9901 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
     9961 ctggtgcgcc aggtgcagca ttcacagctg gagttggtcg ccaactttgc cgatatctcg
    10021 ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc
    10081 gatcgcatca tcgctcatgt tgacggcgtt ccggtactga ccagtcagta cggcaccctc
    10141 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
    10201 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
    10261 acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg
    10321 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
    10381 tgataatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca
    10441 aagagctgtt gcgtctgacg caagggtccg tcgtggcgct ggacggtctg gcgggcgaac
    10501 cactggatat tctgatcaac ggttatttaa tcgcccaggg cgaagtggtg gtcgttgccg
    10561 ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcgaatg cgtcgcctga
    10621 gccgttagtg atgaataacc acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc
    10681 actgctgcag gtcagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
    10741 gctggtaaaa cggttgggat ttgccccgaa acgcactggc gttaacggtc tgaaaattag
    10801 cgccagtgct tcgctgggcg cacgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
    10861 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttcagc
    10921 accaacagaa gagataccgc agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg
    10981 tagcgggaga tcctgatgcg tcgtttgttg tctgtcgcac ctgtccttct ctggctggtt
    11041 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctgcc tggcggtgga
    11101 caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac gtttattccg
    11161 gcaattttac tgatgatgac cagtttcacc cgcatcatca ttgtttttgg tttattgcgt
    11221 aacgcgctgg gaacgccctc cgcgccacct aaccaggtat tgctggggct ggcactattt
    11281 ttgacctttt ttattatgtc accggtgatc gacaaaattt atgtcgatgc gtaccagcca
    11341 tttagcgaag agaaaatatc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
    11401 gagtttatgc tgcgtcagac ccgtgaggca gatttagggt tgtttgccag actggcgaat
    11461 accggcccgt tgcagggacc tgaagccgtg ccgatgcgca ttttgctccc ggcctacgtg
    11521 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcattcc ttttttgatt
    11581 atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt tcccccagcc
    11641 accattgctc tgccctttaa actgatgctg tttgtactgg tggatggttg gcaattgctg
    11701 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
    11761 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc gctgttgctg
    11821 gtggcgctgg tcacgggtct tatcatcagt attttgcagg ccgccaccca gattaacgaa
    11881 atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccattat tattgccgga
    11941 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    12001 atcatcgggt agccgtacta tgttgcaggt gacaagcgaa caatggctat cctggttaag
    12061 cctgtacttc tggccgttac tgcgcgtgct ggcgctgatc tccaccgcgc cgattctgag
    12121 cgaacgcagc gtaccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12181 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12241 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12301 tgtacgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacgtttgt
    12361 cgatccggcc agccatctca atatgcccgt tttagcgcgt atcatggata tgctggcgtt
    12421 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    12481 tcacaccctg ccgattggtg gcgaaccgtt gaacagcaat gcgtttctgg cactcaccaa
    12541 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgct
    12601 gctgacactg aatctggcat taggtttact taatcgtatg gccccgcaat tatccatttt
    12661 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    12721 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    12781 tattagtgaa ttgccattaa tataattccg taacgtttat catgttatcc taaggattat
    12841 ccgaaaaata atacctacga acattttcca ggatactcct gcagcgaaat atttgtttta
    12901 agctcactca catatcgcaa catttacttt actttaagac aattccaggc aaattataca
    12961 aaactttacg ggatagtaag tccgcctgaa aaatcgcgag agtggcgcat taggtgaccc
    13021 atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg
    13081 agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt
    13141 taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg
    13201 ttgatgacct tgccatagct tgtgattcac agcgcccttc agtggtgttt attaatgagg
    13261 actgtttcat ccatgatgct tctaacagtc agcgcatcaa gcacatcatt aatcaacatc
    13321 ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat
    13381 tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct ctcgacgata
    13441 tccttggcga tattctgaaa aaagagacaa cgataacctc ttttttaaat atgccgacgt
    13501 tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca
    13561 ttcaaatctc tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata
    13621 ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg
    13681 ataatgtgac taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc
    13741 caccagtcag gctgaattag attactctgc tttctccaca aagataccgt cctgatgccc
    13801 tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt
    13861 gccttcacta aactcctcaa ctgccagtac cacgccagga cgacgcggac cgccatccgt
    13921 tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt
    13981 agaacaattt cgtttttctg gcagcgccag gcgcgcgtga gtgctgattt tctcgacggt
    14041 ctatacttaa gagatgccag gcggacttaa cgactggcgg caacaacaga gtaacggttg
    14101 cgaggaaaga tgatgaaaac cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc
    14161 gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc
    14221 agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa
    14281 ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga
    14341 atgcacaata aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa
    14401 gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt
    14461 ttttcgctga caagaaaata ttcgtgatat gaatgattaa cgctacttat gaatagaaat
    14521 gtgacgcgcg tcaccccttg gtggctctgg cgtcaacggg atcgcttccg aaaaaatcat
    14581 ctttgataat ttgtgctgtt ttgagaatca tcctggtaag gggagtactg cgcaaccatg
    14641 ctttcaattc aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt
    14701 catagttatg actggcaacc agctgccccc tggctcagcc gtttacgcga agcgaatgtt
    14761 cccgtcattc tctgtagcag taagacatca gcggaaatgt tgtacttgca aaaaacgttg
    14821 gggctacaag gtttaccgct gattgccgaa aatggcgcag tgatccagct tgctgagcaa
    14881 tggcaggata tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatcagc
    14941 caggtattaa atacgctacg cgagaaggaa cattttaaat tcacgacttt tgatgatgtc
    15001 gacgatgcaa ccatcgccga atggacggga ttaagccgta gccaggcggc gctgacgcag
    15061 ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt
    15121 accgctcgtc tgaacgaact gggcttacag tttatgcagg gtgcgcgctt ctggcacgtc
    15181 ctggatgcct ctgccggaaa agatcaggcc gccaactgga ttatcgcgac ctatcaacaa
    15241 ttatcaggca aacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccctta
    15301 ctggaggtaa tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtgcatctg
    15361 catgatgagg atccgacccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt
    15421 gaagggctgg accatttttt ctccgctcgt taagcgttat cgctcgcgca tacccgatta
    15481 cgcccggcct gtttagcgag ataaagccga cggtcagcca gtgactggag ttgttcaaaa
    15541 tcataatcac cggtttcctc gctgctactt acccccaacg aggcactgat gcgtatcgtc
    15601 gtactcttag caatcaacat ctctttttca tttaacttca ggcgaatacg ttctgcgact
    15661 tccgcagcct ccgtcagact cgcgcctggc agaatcacac aaaactcctc accaccgacc
    15721 cgcccggcaa cgtcctgcgc acgcaaggaa ctgctaatta atccagcagc atgagaaaga
    15781 acacggtcgc ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaaggtcg
    15841 acctgaatga cagaaaaagg atgttggtgc gtctgacaca atttcgcgag cggacgggct
    15901 ttttcaaaca gtgcgccacg attatataaa cgggttaagg tgtcgtgcca cgcctgccac
    15961 tgcaacgaac tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag
    16021 atgagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg
    16081 aaatcgccgc gcacgccttc gcttagcgta tggacgcgca ccagcacacc gtcgaaatgg
    16141 tccagacgtt cccagctaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt
    16201 tccattgcct gcgccagcaa ggccagttcg cgaggatcaa aaatattccc tgttggatgg
    16261 tcgggattgg aagaggtcaa taatctcagc ttactgtcat agagctgata ctcaccatcg
    16321 aggtttttat cgatggcgtt tcttaaaaat tgctgcatag tacgcacagg aatactcatc
    16381 cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt
    16441 tcagtattgc tggcatgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt
    16501 tctcgttgtg aatggccgat aaaccagggt tgggtgacat agccgtaata acgcgtcggg
    16561 acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg cgagacatac
    16621 atcgcctgtt caaccatcga cgaggagttg tgtgccagtc gcagcaagta accaacttcc
    16681 agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggag atttccctcg
    16741 ctgactaatg catccgatac gccattgact ggcagggtgc gtcgtcggtt gagttcgatt
    16801 ttccaggcgt gctcgtcgcg atgctgttcg aactcggtta cagcatcacg cagagaggtg
    16861 aaatccagtg gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg
    16921 acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg
    16981 ctggagatat aggcatcttc cagcaccact acttcacgcc aggtgagcaa ggtggaaaaa
    17041 agcaatacca cgataaagca gagattaacg acatgaccag gccccaggcg gcgtgcgagt
    17101 tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac
    17161 ctgctttttt atgattctgg cataacctgg cgatagcgta ccgcaaaaca gcgataattt
    17221 tataatggtg gcatgataag acgcgactat cattcattgc aggcattggc acaccaggcc
    17281 ggatgtgatc cggcctggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc
    17341 cctcccccgg aacaagctcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat
    17401 accaacgact ctcaaccata ccgctggagt atccagtgac aatcatccgc gggccgccct
    17461 ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga
    17521 aacttgttca ccttagttga agatggcgaa ttttgcataa tagcctgtgc gagtattaat
    17581 gtgcctgacc gcgcattttc gctacggctt tcactcccag caccacgatg ccgccgatga
    17641 taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct
    17701 gcccggcgaa atgttcaatg gcgtgatgca gcggcgcaat accatgtacc acaatcccgc
    17761 cgccgacgag gaacatcgcc agcgtgccga caatcgataa cgccttcatc aaccagggcg
    17821 caatgatcaa taacccttta cctaatgcct gcatcagcgc gctggatttt tccgccagcc
    17881 aataccccag gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca
    17941 ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgct tcggccacaa
    18001 tccccagcgt gatggcgacg atttcggcag acaatataaa atcggtacga atcgcccctt
    18061 ttattttgtc cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct
    18121 ggctctgcgc cggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta
    18181 ctccttcaaa gcagagaaac gcgccaccaa tcatcaacag aggcgtaatc gcccacggga
    18241 taaacgcact gatgatcagc gccagcggca ccagaatcac tttattaatc agcgatcctt
    18301 tcgccacgcc ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg
    18361 cattgagcga taagtcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca
    18421 tcacggagat atcgtccagt agtgtggcga tatcatcgag caacgtcagt aaactactcc
    18481 cggccaaaag cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg
    18541 gcagctctga tacttgcttc acaggtcaac atttttttaa caatcaagtg caattatatc
    18601 tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt ccaccgtgag
    18661 ggattatgcg tttcggccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca
    18721 tttggggctc aacctatttt gtcattcgga ttggcgtgga aagctggcct ccgttaatga
    18781 tggcaggcgt tcgattcctg gcagccggta ttttattgct ggcatttttg ctactgcgcg
    18841 gacacaagct ccccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc
    18901 tggctgtcgg taatggcatg gtgacggttg ccgagcatca aaatgttcct tccggcatcg
    18961 ccgccgttgt ggttgcaacc gtgcccctct ttaccctgtg cttcagccgc ctgtttggca
    19021 ttaaaacgcg caaactggaa tgggtgggta tcgccattgg gcttgccgga atcatcatgc
    19081 tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct
    19141 cgattagctg ggcgtttggc tcggtttatg gctcgcgcat taccttacct gtagggatga
    19201 tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgcg tcgatgattg
    19261 caggtgaaaa actgacggcg ctcccttccc tttcaggctt ccttgcggtc ggctatctgg
    19321 cgctgtttgg ttcgattatc gccattaacg cttatatgta tttaatccgt aatgtcagtc
    19381 cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg ctgggtacgg
    19441 gactgggcgg agaaacgctg tcgaagattg aatggctggc gctcggcgta attgtcttcg
    19501 cggtggtgct ggtcacgttg ggaaaatatc tctttccggc aaaacccgta gttgcgccag
    19561 tgattcagga cgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca
    19621 ccttcgccgc agatccactc ttccagacgc tcgctaagcg cttcatccgt cagcttctcg
    19681 cgcccacgta acgcacactc ccagacaatc agtacgcgcc agccaagctc ctgcaagcga
    19741 ctgatatcac ggcgatcgcg ctcaacattt ttacctatct tctccagcca gaactcggtt
    19801 cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca gccgtgggta
    19861 aatatcacgc aacgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc
    19921 tgaacgcgaa acgccaggcc ctgcccggtt aacagactgg cgaggcgctt ctcaatcgcc
    19981 gtatcacgcg tggcaatcgc gcgcatattt ttgctgcgag tggccttatc gtgaacgtcg
    20041 gccatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag
    20101 ttttgccacc gcggcaaaga ccggcacgac caccgagtta ccgaactggc gataggcctg
    20161 agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg
    20221 cgcgcattca cgaggcgtta accgacgtgg acgatgttgc tgattctgcg gatcgtcaaa
    20281 gtctttctca cctttggcca tatcccagcc gcgatcgatt aaaatttccg cgccatcttt
    20341 gtaataacgc gcagacagcg tacgcgtgac gctttgcgga ttgttcggat aaaccattcc
    20401 ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga ggtacttcca
    20461 cagcaccggc gtcaggatat atttcgcctc gaccatcggg tccagcagct gcgccagcgt
    20521 cactcgctgc gcagggaaac attcgctgat atcacgcagg gtaaaatcgg ctttcagatt
    20581 cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca gaaaatgttt
    20641 gccgtcgatg attttcggat cgtccggccc gttatcttct gcatcagcca cgtcatagcc
    20701 cagttcgtcc agcgtctgca tgatgatgcg gaacgtttta ccctggtcgt gacttttcag
    20761 gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tacgcaccac
    20821 atcaaaaaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt
    20881 ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc
    20941 gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttt
    21001 atggctgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata
    21061 atgattggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg
    21121 tccgccaatc gattcaaaac cgcgacggat gccgccaatt ccggcaaata gatcgataaa
    21181 gcgaaacgca taatgcggat gatgtgccgg tggtttgggt aacaacgttt gcagatgggc
    21241 gaactctttc tcacttaaac gatgccatgc cgagtcattc gccagcgcac gttttaaaat
    21301 tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagcg ttttaacatc
    21361 ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc
    21421 ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta
    21481 acataattac agccatttag ggccacgcgc ttatcccgcg ggtgaaaacg catctatcac
    21541 cttgtgatcg acaccttcat tctcccccgc cagttcggca ctgagcttcg ccataaactc
    21601 gactaaaaaa tgcgcgttgt gctgcgccag ctgcttgcct cttacggttt gcatggtttg
    21661 cggtagcttc agcaacttag tctggaaatg atccagcgcg tagcgtttat catcaagcgg
    21721 gcgatgctgt gcgagcggat cttcgccatc aaacagcgct acgcccaatg cccctgaaac
    21781 ggcaaacacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac
    21841 gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc
    21901 atgacaaacg gcctcgattt tctccgccgg aaattgctcg aactcttcac ggagcaggcg
    21961 acgcgtctct tctgccgcca ggattgaaga acgctgcctt tgcggatgat ttttcgccag
    22021 gctcacaata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc
    22081 tgccgccagc ttctgtgccg tagcccagac gcggcgaaaa tgacacacgt catgggccgc
    22141 gtcctggtgc tgatgatggt ttttcaacca gttttcgaac tgtgcctgcc agtgttgtaa
    22201 gtccatacgc gctccgttgc caaagagtcg ccaccttagc agtttttatt catagcgtct
    22261 gctacgtgcg ggacgaggtc gggtgtacca ggcaatgctg cctaacaatg ccccgaccag
    22321 agccataaat aaaaatccga ccagtgcccc taaccacttt cccgcggtag agcctaaatc
    22381 gctttcaaat cccgacacgg gggcatcggg gatccggctc atcacccaga tgtaacgcgc
    22441 cattgcccag acgaaatagc cacaccatgt atagaaagcc cacaaagcca gtttgccgcc
    22501 agggctgcga gagagtctct cttccatctt tgtcgaaccg tattccattt tgagataacc
    22561 cggaaaattc agacttagta ctattatgtg ataaaagtca catttttcca cattggatga
    22621 gccagtcaag actcaatcat tttttgaaca aatagataaa ttttgcgcaa cggcagtgaa
    22681 tacaggaaaa atcgttttga gcgattttat ttatctttga tatcg
//
LOCUS       DAZUFB010000073        22449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZUFB010000073 DAZUFB010000000
VERSION     DAZUFB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.073"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..916
                     /locus_tag="ACHY3M_003223"
                     /pseudo
     CDS             217..916
                     /locus_tag="ACHY3M_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1092..1682
                     /locus_tag="ACHY3M_003224"
     CDS             1092..1682
                     /locus_tag="ACHY3M_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFS4318085.1"
                     /translation="MKIISTVIQTPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFED
                     MNDSSALYKKYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVP
                     GIPLSDIDTADIPDNLESLYLQLICKLNELSIIHYDLNTGNMLYDKESNSLFPIDFRN
                     IYSEYYSATKNDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1721..1938
                     /locus_tag="ACHY3M_003225"
                     /pseudo
     CDS             1721..1938
                     /locus_tag="ACHY3M_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2048..2311
                     /locus_tag="ACHY3M_003226"
                     /pseudo
     CDS             2048..2311
                     /locus_tag="ACHY3M_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            2534..2659
                     /locus_tag="ACHY3M_003227"
                     /pseudo
     CDS             2534..2659
                     /locus_tag="ACHY3M_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(2719..3225)
                     /gene="yciE"
                     /locus_tag="ACHY3M_003228"
     CDS             complement(2719..3225)
                     /gene="yciE"
                     /locus_tag="ACHY3M_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4318086.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(3271..3771)
                     /gene="yciF"
                     /locus_tag="ACHY3M_003229"
     CDS             complement(3271..3771)
                     /gene="yciF"
                     /locus_tag="ACHY3M_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4318087.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3857..4036)
                     /gene="yciG"
                     /locus_tag="ACHY3M_003230"
     CDS             complement(3857..4036)
                     /gene="yciG"
                     /locus_tag="ACHY3M_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS4318088.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(4417..5223)
                     /gene="trpA"
                     /locus_tag="ACHY3M_003231"
     CDS             complement(4417..5223)
                     /gene="trpA"
                     /locus_tag="ACHY3M_003231"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFS4318089.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5223..6416)
                     /gene="trpB"
                     /locus_tag="ACHY3M_003232"
     CDS             complement(5223..6416)
                     /gene="trpB"
                     /locus_tag="ACHY3M_003232"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFS4318090.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(6428..7789)
                     /gene="trpCF"
                     /locus_tag="ACHY3M_003233"
     CDS             complement(6428..7789)
                     /gene="trpCF"
                     /locus_tag="ACHY3M_003233"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFS4318091.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(7790..9385)
                     /gene="trpD"
                     /locus_tag="ACHY3M_003234"
     CDS             complement(7790..9385)
                     /gene="trpD"
                     /locus_tag="ACHY3M_003234"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFS4318092.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(9385..10947)
                     /gene="trpE"
                     /locus_tag="ACHY3M_003235"
     CDS             complement(9385..10947)
                     /gene="trpE"
                     /locus_tag="ACHY3M_003235"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFS4318093.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(10969..11062)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(11039..11083)
                     /gene="trpL"
                     /locus_tag="ACHY3M_003236"
     CDS             complement(11039..11083)
                     /gene="trpL"
                     /locus_tag="ACHY3M_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFS4318094.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            11221..12102
                     /gene="rnm"
                     /locus_tag="ACHY3M_003237"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             11221..12102
                     /gene="rnm"
                     /locus_tag="ACHY3M_003237"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFS4318095.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            12099..12719
                     /gene="yciO"
                     /locus_tag="ACHY3M_003238"
     CDS             12099..12719
                     /gene="yciO"
                     /locus_tag="ACHY3M_003238"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4318096.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            12820..13692
                     /gene="rluB"
                     /locus_tag="ACHY3M_003239"
     CDS             12820..13692
                     /gene="rluB"
                     /locus_tag="ACHY3M_003239"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFS4318097.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(13732..14322)
                     /gene="cobO"
                     /locus_tag="ACHY3M_003240"
     CDS             complement(13732..14322)
                     /gene="cobO"
                     /locus_tag="ACHY3M_003240"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS4318098.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(14319..15077)
                     /gene="yciK"
                     /locus_tag="ACHY3M_003241"
     CDS             complement(14319..15077)
                     /gene="yciK"
                     /locus_tag="ACHY3M_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFS4318099.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            15297..16346
                     /gene="sohB"
                     /locus_tag="ACHY3M_003242"
     CDS             15297..16346
                     /gene="sohB"
                     /locus_tag="ACHY3M_003242"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFS4318100.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(16382..16633)
                     /gene="yciN"
                     /locus_tag="ACHY3M_003243"
     CDS             complement(16382..16633)
                     /gene="yciN"
                     /locus_tag="ACHY3M_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFS4318101.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            17013..19610
                     /gene="topA"
                     /locus_tag="ACHY3M_003244"
     CDS             17013..19610
                     /gene="topA"
                     /locus_tag="ACHY3M_003244"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4318102.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            19820..20794
                     /gene="cysB"
                     /locus_tag="ACHY3M_003245"
     CDS             19820..20794
                     /gene="cysB"
                     /locus_tag="ACHY3M_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFS4318103.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            21113..21253
                     /gene="ymiA"
                     /locus_tag="ACHY3M_003246"
     CDS             21113..21253
                     /gene="ymiA"
                     /locus_tag="ACHY3M_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFS4318104.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            21256..21423
                     /gene="yciX"
                     /locus_tag="ACHY3M_003247"
     CDS             21256..21423
                     /gene="yciX"
                     /locus_tag="ACHY3M_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFS4318105.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            21537..21632
                     /gene="ymiC"
                     /locus_tag="ACHY3M_003248"
     CDS             21537..21632
                     /gene="ymiC"
                     /locus_tag="ACHY3M_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFS4318106.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            21796..>22449
                     /locus_tag="ACHY3M_003249"
     CDS             21796..>22449
                     /locus_tag="ACHY3M_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFS4318107.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT         5621 a         5449 c         5715 g         5664 t
ORIGIN      
        1 gtcatgtccg gtggatgttt gttaggaatg ttcagacagg tttattttga atttacacag
       61 aatcctaaac aggttcgaga attaagaaag aggttgtatg tttagcataa gaaccctact
      121 acctattagc gccagcgtat cagttccgac aaaacaatct cggtaatgct gccaacttac
      181 tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc catcagatgt
      241 ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc cggacatcag
      301 cgctatctct gctctccttg ccgtaaaaca tggcaactac agttcactta caccgcctct
      361 cagcccggta cgcaccagaa aatcattgat atggccatga atggcgtcgg atgtcgcgcc
      421 agtgcacgca ttatgggcgt tggcctcaac acggttttac gtcacttaaa aaactcaggc
      481 cgcagtcggt aacctcgcgc atacaaccgg gcagtgatgt gattgtctgc gctgaaatgg
      541 acgaacagtg gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg
      601 acaggatacg gaggacggtt gtggcgcacg tcctcggtga acgcactctg gccacactgg
      661 agcgtcttct gagcctgctg tcggcctttg aggtcgtggt atggatgacg gatggctggc
      721 cgctgtatga atcacgcctg aagggaaagc tgtgcacgtt atcagcaagc gttacactca
      781 gcgcattgag cgacataatc tgaatctgag acaacacctg gcaaggccgg gacggaagtc
      841 actgtcgttc tcaaaatcgg tggagctgca tgacaaggac atcgggcatt atctgaacat
      901 aaaacactat cagtaagttg gagtcattac ctaaaggcct ctctacactg tgcttgtatg
      961 gggcttccct gatcatggcg gttatctatg taatagcata tgtacttacg tggtttctga
     1021 ggtggtagcg cgtaatatta ttttatgtaa aaatatcttg taatctttaa atttcataac
     1081 aagggcatgt catgaaaata atatccactg ttattcaaac acctttccca tttgagaata
     1141 ataattctca tactggcgta gtaacggagc ctattctcgg taagttaata gggcaaggtt
     1201 caacagcaga aatctttgaa gatatgaatg attcgtctgc tttatataaa aagtatgatc
     1261 ttgttggtaa ccagcacaat gaagttctgg aaatggctag gcaagaatct gcccttttta
     1321 atacttttta tggcgatgac gcatcagttg ttatacagta tggcggtgat gtgtatctcc
     1381 gaatgctacg cgtgccaggg attcccctta gtgatattga tacagctgat attcctgata
     1441 atctagagag cctttatctg cagttgatat gtaaattgaa tgagttgagt ataatccatt
     1501 acgatcttaa tacaggtaat atgttgtatg ataaagaaag taatagttta tttccaatag
     1561 attttcgaaa tatttattct gaatattact ctgcaaccaa aaatgataaa gagattatcg
     1621 atcgacgatt acagatgcgt acaaatgatt tttattcatt attaaacaga aaatatttat
     1681 aagtgctata ttcatgaatg aaaaatagct atatgcgtac attctcaacc tgcccgtcac
     1741 gtagcaggtt gaatcacttt ttcttgataa agggctggtc ctgccaccgt tgttgttggg
     1801 tgcgttaaaa atacactact aatatgcccg gcatgcgggc aacagagtcc tgttcacgat
     1861 cgccgccacc ggaaatggcg ctgcctcgat acctgccagt tcatggcact attcgaagcc
     1921 gatgttcccc acgttatatg gagtaatcat tggggttatg ccgcagcacg ttgtatgcaa
     1981 gaatgtgctg cggctggttt gtgagctttc gatagtggtt gttatttttg cccttatttg
     2041 ttccggaggc cctggttcaa tggtccgtct gccccctgtg gtgatgtcag caaaatcagc
     2101 cactgcgcga accacaatag cccgggaaga tgctgaagat caccaggtaa agctgtcagc
     2161 gcagaaactg gaagaactgc tcgcatcaat ggttaaggat gaggttgatc gcaatgatgg
     2221 gatttattga cgtcagcgag agcagaagga agaactgaat aacctgaatg atttacgctc
     2281 aatcagagcg atgatgattg gcagtaatta gcggtcaatt tcgggttttt tggctgtgcg
     2341 ctccccgccc agcattgcgc gtcaattagc cccaaaaaaa acgaaaaagt tacaatcaag
     2401 taaaaaattc cttctgcaaa actaaaaaag atttcaataa aatcaggcag ttaaaaacat
     2461 aaaaaaaagt acaaaaaagc atctcaaagt cttgtttttt gcttcaaata caattggtta
     2521 ttgtgttttt tgaaacctac cagtgtggta catggatatc gataccaccg ccaattataa
     2581 gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc
     2641 agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg ggtcaataag
     2701 gacatggtat aaagggtatt atttcttcgc ttctacgcca tcagtttcag aacgaattaa
     2761 aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt gcttttcctc
     2821 atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt ttgctgctgc
     2881 taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc taatagagcc
     2941 tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg cagccatttt
     3001 actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa tagtttccag
     3061 ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc gtagttcagg
     3121 ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct tttccattgc
     3181 gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa tgtcctcttt
     3241 ataaaatgac taaaagttaa aattcatatt tcaggcttta ttttcggctt tcttatttac
     3301 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtttc
     3361 tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg ctaatgtccc
     3421 gtaactggca atctcataat gctcgacttt ctgcgctgcg gcaatcagtg cggcatcacg
     3481 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
     3541 tgccacacat ttcatgcgct taattttcag gttcgattcg gactccacaa cttgatcaat
     3601 acgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
     3661 atttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt ctgcgctgta
     3721 ggtatctgaa agcaggtgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
     3781 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
     3841 aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt tgaccgcctt
     3901 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
     3961 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
     4021 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
     4081 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
     4141 agaagataga ggaaaaatat attttgagga acattctgga tatattaaca attacctgag
     4201 gaataagtga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     4261 tttgattata ttactgttgg gcggaaaaat gacctaagtt gacgttcgac cggggtaagc
     4321 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     4381 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     4441 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     4501 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     4561 ttttacctga tccggggcgg aaataccaaa tccctgcaat ggaggtgcag cgttgtactc
     4621 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     4681 gcctgctcgt gacagcaaat aggtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     4741 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatgac gcaacgcggc
     4801 ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg aatcgacgcc
     4861 gactttttcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     4921 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     4981 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatcgtcgg
     5041 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     5101 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     5161 aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     5221 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     5281 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     5341 aacgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     5401 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     5461 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     5521 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     5581 tagataccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc gataccgtga
     5641 ccacctggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     5701 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     5761 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acgcacaatg
     5821 gtcggataag gatgcgggcc agctgcggtg cccagcatat agtgcgcggt ttcgtaacta
     5881 ccggaccagt cgcgcagcgc ctcgttacag gcatctttca gcgtcgcgga accgctatgc
     5941 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttca
     6001 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     6061 gacgccacgc catgctgacc ggcaccggtt tcggcgatga tttcggtttt acccatccgc
     6121 ttcgccagca acgcctgccc cagcacctgg ttagttttat gcgcgccgcc gtgcagcaaa
     6181 tcttcacgct tgagatacag cgtggtgttc gtcccggctg taatgttctg gcatttggtc
     6241 agcgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgagc ctgaaattca
     6301 ggatcttttt gcgcactgac aaaagcttct tccagctggc gcagagcagg catcaggatt
     6361 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
     6421 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
     6481 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
     6541 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
     6601 catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
     6661 atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
     6721 agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc tgttcttcat
     6781 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
     6841 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
     6901 cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
     6961 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
     7021 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
     7081 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
     7141 cggattcgct gattaccgtc acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
     7201 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccca
     7261 atgcaatggc acgctccagt tcctcttcat tactgacttc ggtcagcaca cccatctcca
     7321 gactgtgggc gacggcggca agctggcgat attgttcgtc atccagtact gaaagcatta
     7381 ataagcaggc atcagcctgg taatagcgcg ccagatagat ctggtaagga tcgataatga
     7441 agtctttaca taaaatcggc tgcggggcga tttggctgac gatggggagg aaatcaaagc
     7501 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
     7561 tggcggcaat gcgtgctgga tcgaaatcat cacggatcac gccttttgac ggcgacgctt
     7621 tcttgcactc cagaataaac gccgtgcgcg caccctgtag cgcatcataa aaatgtcgcg
     7681 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
     7741 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
     7801 ccgccagtgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
     7861 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
     7921 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac
     7981 ggttttcttc cggtgttccg cctgccagtt gctcctggtg gtagggagtc aggccaaaat
     8041 cttcagcggt caattgatag ctcttaattt cgccgtcatg cagctcggca acgattgtcg
     8101 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
     8161 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
     8221 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
     8281 ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatac ttcggcgcaa
     8341 agaggaaaca tacacctaac tcatccagcg cctggcgcga tttatcggcg ttcatatcaa
     8401 gattaatacc gaacgccgcc agcagatcgg acgaaccaga tttactggag acgctacggt
     8461 tgccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
     8521 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
     8581 ggcgcgggaa cggcgcggcg ttttccagta gcgcggttgc tgctccggcg atctcgttcg
     8641 ggtgctcacc gcgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
     8701 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
     8761 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
     8821 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
     8881 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
     8941 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
     9001 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
     9061 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
     9121 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
     9181 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
     9241 tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
     9301 ccacattatg cccattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
     9361 tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
     9421 agcgcgcagt acagcgcggg ctttattacg ggtttcgtcg gcttccgact gcggaacaga
     9481 atcaaggact acgccagcac cggcttgcac ggtggcgata ccgttttcca ccagcgccga
     9541 gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc
     9601 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg
     9661 cgcaccactt aacgttccca tattcataca ggcgcggtaa gcgtgcaggg cgtcgagatc
     9721 gtggcgcagc tcaccgacca cgcgggagac caagtgcatc acgtaagagt aacggtcaac
     9781 tttggtgagg tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca gatcattacg
     9841 ggcgagatcc accagcatca gatgttcaga aagctcttta tgatcggtac gcatttccag
     9901 ttcgatgcgg ctgtcgaggt ctctgtccag tgaaccatcg gcacgacgac cgcgcggtcg
     9961 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcgtcatact tgagcgaact
    10021 ttccggcgac gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
    10081 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
    10141 acggcgagat ggcaccacct ggaaaatttc tccggcgcga atcgcttttt gcaacaaacg
    10201 cactacgcca ccgaactctt catcgctctg gttacattca caacgcatat gcggcacgga
    10261 aaccaccggc agcggtggcg cggtttcggt cagttgctgg cgaagatcgt tcaggcgagc
    10321 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tgcgggtgct
    10381 ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga aatcaggaca
    10441 gttattttcc gctgacaatt gcggtaaatc ttcaaacccc gccacaaggt cataagagaa
    10501 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcagca tattctgcaa
    10561 taagcggaaa gcgtcaaaaa ccgaaaggga gcataagcga gcgtcttcat ccagcagtgg
    10621 actgacaggg gggaagcgca gcacacggca gtttggtaat tgttcatttt ccatacccgc
    10681 aggcagggcg ttatccagta gtgtcagcag ggcttcgccg ttgccggaaa gtgcctggat
    10741 tgtgacagtg tcacctaaag ctgtaatgcg cagcgcactg tctaccagca gcaggctttt
    10801 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgatcccc
    10861 acacaactgg tgaaaaagcg cggtgggatt gtcgcgataa gcgccttcgc aggttagcag
    10921 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc
    10981 gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc
    11041 aggaagtgcg ccaccaacct ttcagtacga aaattgcttt cattgtcgat acccttttta
    11101 cgtgaacttt cgtactagtt aactagttcg atgattaatt gtcaacagct catttcagaa
    11161 tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
    11221 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
    11281 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
    11341 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
    11401 tcgggactgg cgttgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
    11461 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
    11521 gcgcagcaga cagaacggcg caatcagcgg gcgcagctga ttgccgaaag acttgaaaaa
    11581 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
    11641 cgcggtcatt ttgcgcgttt ccttgttgag tgcggcaaag ccagttcaat ggcggatgtc
    11701 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
    11761 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
    11821 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
    11881 ggtgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgtac ccagctggcg
    11941 gcccttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
    12001 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
    12061 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
    12121 atcctgataa cccacagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
    12181 gggtgattgt ttatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa
    12241 acgcgatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
    12301 tgtgtcgcga tctttctgaa ctctcgacct attcatttgt tgataacgtt gcgtttcgtt
    12361 taatgaaaaa caacacgccg ggcaactata ccttcatctt gaaggggacg aaggaagtgc
    12421 cacgccgtct gttgcaggaa aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
    12481 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
    12541 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
    12601 tagatttgat tattcacggc ggctatctcg ggcagaaacc gacaacggtt attgatctta
    12661 ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
    12721 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
    12781 tgttcgacgc ctgtgaaggc gacactaaaa ggaagctcta tgagcgaaaa attacagaaa
    12841 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
    12901 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
    12961 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
    13021 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
    13081 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
    13141 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
    13201 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
    13261 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
    13321 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
    13381 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
    13441 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
    13501 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagctaccg
    13561 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggcgaatcag
    13621 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcggacgt
    13681 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attagtaatc
    13741 aatccctatc tgcgctttta caccggcatc aaacgcatgt ttgacggggc gtaattcact
    13801 taccgtatct gccagttcaa gaatatcccg atgacaacca cgacccgtga taatcaccgt
    13861 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
    13921 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
    13981 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
    14041 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
    14101 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcctactt tttttccgtg
    14161 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
    14221 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
    14281 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
    14341 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    14401 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    14461 aaggcgctgg cacgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    14521 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    14581 gcatatgcac cccagttagc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    14641 gaaccggcgt ccgatttgag taataaagga agcagcgcct gggtgagcaa aaaggtggca
    14701 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    14761 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacggta
    14821 atgcgctgtg ccagttgttg gcaatcttcg gacgtgcagg tcagcaaatc gagaataaac
    14881 cactgtggct gacgtccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    14941 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    15001 cggccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    15061 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    15121 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    15181 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    15241 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    15301 aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg ctagcgattg
    15361 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    15421 tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg gccgcggcgc
    15481 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    15541 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    15601 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    15661 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    15721 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    15781 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    15841 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    15901 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    15961 aactgcacac tgccgggcag tataagcgta ctctgacctt gctgggtgaa aataccgaag
    16021 aagggcggga gaaattccgc gaagagttga acgaaacgca tcagttgttt aaagattttg
    16081 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    16141 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    16201 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    16261 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    16321 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    16381 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caagtgtttg aacatattaa
    16441 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    16501 ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct
    16561 catgctcacg aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    16621 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    16681 cagacaaata taccacgctg gtggcaagag cgccttactg gtaactttgg attttgcatg
    16741 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    16801 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    16861 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggt tgatatccgc
    16921 agagaacgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    16981 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    17041 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    17101 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    17161 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    17221 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag
    17281 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    17341 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attgggggtg
    17401 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    17461 catttaataa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    17521 ttatggaccg cgtggtgggg tatatggttt cgccgctgct atggaaaaag atcgctcgtg
    17581 gtctgtctgc cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg
    17641 aaattaaagc gttcgtgccg gaagagttct gggaagtcga tgccagcacg accacgccat
    17701 ctggtgaagc gttggcgtta caggtgactc atcagaacga caaaccgttc cgtccggtca
    17761 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    17821 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    17881 aacaagctgc cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg atggcgcagc
    17941 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    18001 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    18061 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    18121 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    18181 tgtaccagtt aatctggcgt cagttcgtag cctgccagat gaccccagcg aaatatgact
    18241 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    18301 gttttgatgg ctggacaaaa gtgatgcctg cgttgcgtaa aggcgatgaa gatcgcatct
    18361 taccagcagt taataaaggc gatgctctga cgctcgttga acttacacca gcccagcact
    18421 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagagctg gaaaaacgtg
    18481 gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctacg
    18541 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    18601 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    18661 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    18721 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    18781 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    18841 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    18901 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag
    18961 atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg
    19021 acagctatct catcgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg
    19081 acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg
    19141 agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg
    19201 cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac
    19261 caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt
    19321 tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc
    19381 gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa
    19441 aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc
    19501 gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg
    19561 gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc
    19621 tgtcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc atattatttt
    19681 ttcttcacac ctatacacta aggctataaa tgatatagtg gttatagtta gcaccttttt
    19741 tattattaaa tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt tctgatggcg
    19801 tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa
    19861 tcataacctg aatgtctcat caacagcgga aggactttac acatcacaac ccgggatcag
    19921 taaacaagtc agaatgctgg aagacgagct aggcattcaa attttttccc gaagcggcaa
    19981 gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc gcgaagtcct
    20041 gtcgaaagtc gatgccataa aatcggttgc cggtgagcac acctggccgg ataaaggctc
    20101 gctgtatatc gccaccacgc atacccaggc acgctacgcg ttaccaaacg ttatcaaagg
    20161 ctttattgag cgttatcccc gcgtttcttt gcatatgcac cagggctcgc cgacacaaat
    20221 tgctgatgcc gtctctaaag gcaatgctga tttcgctatc gccacagaag cgctgcatct
    20281 gtatgaagat ttagtgatgt taccgtgcta ccactggaat cgggctattg tagtcactcc
    20341 ggatcacccg ctggcaggca aaaaagccat taccattgaa gaactggcgc aatatccgtt
    20401 ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc
    20461 agggttaacg ccgcgtatcg tcttcacggc aacggatgct gacgtcatta aaacttacgt
    20521 ccggttaggg ctgggggtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc
    20581 cgaccttgtg cgtgttgatg ctcacgatat cttcagccac agtacaacca aaattggttt
    20641 tcgccgtagt actttcttgc gcagttatat gtatgatttc attcagcgtt ttgcaccgca
    20701 tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tctaatgaag aaattgaggt
    20761 catgtttaaa gatataaaac tgccggaaaa ataatttctt gcgttatttt cggcaccttt
    20821 tatgtagcga aggtgccgga atatattctc ttttgttact tatttctata ccgtgcacaa
    20881 tatttttatc cctcatattt atagggtaaa ttacctgtaa agtagtgtct aattacccgc
    20941 ctcgcaaact ccagttttca aatatctatt tcaggtcaaa agattgaatt attcactgga
    21001 gacgattcgt aaattcgctg gatttttgac taaagtttct ttaggatttg tctcatcgta
    21061 tgattaatta caccaatcgt tcaacgtctt gctgataagt gatggcgatt atatgaggtt
    21121 agcaatgcct tctggaaatc aggaaccccg ccgcgatcct gaattaaaac gtaaagcctg
    21181 gctggcggtt tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa
    21241 agtgtgggga taactatggt cggtcaggag caactggagt cgtcaccatt atgccagcat
    21301 agtgacaatg aaacagaaac gaaacgggaa tgttccgtcg ttattccaga cgactggcaa
    21361 ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg aagatgatca gccgaaacaa
    21421 taattatcat cattcttatt acccattttt aatgaattaa agggctttta atacaccgca
    21481 gcaataacag cttgagttat ctcaacacaa aataataacc gttaagggtg tagcctatga
    21541 tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct
    21601 gggccgaact cctctggatc attactcact gatccttgac cccgctgcgg cggggttgtc
    21661 atttgctttg ccacaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac
    21721 gcgatgtttg tgttatcttt aatattcacc ctgaagagaa tcagggcttc gcaaccctgt
    21781 cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc
    21841 aaagataaaa cttaccacta ctacagcctg ccgcttgctg ctaaatcact gggcgatatc
    21901 acccgtctac ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt
    21961 aactcggtta ccgaagagga tatccacgcg ctggcaggat ggctgaaaaa tgcccatgct
    22021 gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct
    22081 gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggcgg cgatactgca
    22141 aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt
    22201 tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa
    22261 cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca
    22321 ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagtgaa
    22381 ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc
    22441 actatgatc
//
LOCUS       DAZUFB010000074        22335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZUFB010000074 DAZUFB010000000
VERSION     DAZUFB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.074"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6192
                     /gene="efa1"
                     /locus_tag="ACHY3M_003250"
     CDS             <1..6192
                     /gene="efa1"
                     /locus_tag="ACHY3M_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFS4318108.1"
                     /translation="DRLYHEGYVFSDINTLSRYTLHGLGITGVHTEENLLPAPSSSLI
                     NILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPHELLTPVLGQSVN
                     PLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGTLNSQNLR
                     QLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLP
                     GTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVASSLHFIAEHAK
                     YTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTEDTA
                     HYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLL
                     HKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDA
                     LKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGLSTRIQHT
                     TTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLNGDVLNDY
                     LSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQ
                     QLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHTTSVEVPE
                     VVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKINLAMDIK
                     QLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKALSASARV
                     LELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAASVAFPPLI
                     IATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPERGLLDFSG
                     NKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSISPYSSLA
                     HGYANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVPDSTSRHK
                     RPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRGGSGGLAV
                     QVGGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLKTPDDEVPVMTIT
                     QKGINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTL
                     SSNSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKLNHFAGNFRVHTR
                     DGMTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHDMGWSLTPEVRFQ
                     GGETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRYIIMKPVTTQILP
                     TQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISGIEESLPE
                     TTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQVMTFLPRV
                     SGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYL
                     IFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSLILSEFRR
                     FTISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTT
                     DREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQKEETSKI
                     LYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDLSGRYKSVPMARK
                     ADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVV
                     HSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGRVYSREEY
                     MMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVSKWSRGWL
                     KTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNAPGETRCT
                     LRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6364..6513
                     /locus_tag="ACHY3M_003251"
                     /pseudo
     CDS             6364..6513
                     /locus_tag="ACHY3M_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            6515..7234
                     /locus_tag="ACHY3M_003252"
                     /pseudo
     CDS             6515..7234
                     /locus_tag="ACHY3M_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
     gene            complement(7353..7526)
                     /gene="ghoT"
                     /locus_tag="ACHY3M_003253"
     CDS             complement(7353..7526)
                     /gene="ghoT"
                     /locus_tag="ACHY3M_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFS4318109.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7554..7850)
                     /gene="ghoS"
                     /locus_tag="ACHY3M_003254"
     CDS             complement(7554..7850)
                     /gene="ghoS"
                     /locus_tag="ACHY3M_003254"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS4318110.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            complement(8077..8349)
                     /gene="yjdJ"
                     /locus_tag="ACHY3M_003255"
     CDS             complement(8077..8349)
                     /gene="yjdJ"
                     /locus_tag="ACHY3M_003255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4318111.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(8361..8591)
                     /gene="yjdI"
                     /locus_tag="ACHY3M_003256"
     CDS             complement(8361..8591)
                     /gene="yjdI"
                     /locus_tag="ACHY3M_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFS4318112.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            8772..10403
                     /gene="dcuS"
                     /locus_tag="ACHY3M_003257"
     CDS             8772..10403
                     /gene="dcuS"
                     /locus_tag="ACHY3M_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4318113.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            10400..11119
                     /gene="dcuR"
                     /locus_tag="ACHY3M_003258"
     CDS             10400..11119
                     /gene="dcuR"
                     /locus_tag="ACHY3M_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFS4318114.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            11690..13030
                     /gene="dcuB"
                     /locus_tag="ACHY3M_003259"
     CDS             11690..13030
                     /gene="dcuB"
                     /locus_tag="ACHY3M_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFS4318115.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            13108..14754
                     /gene="fumB"
                     /locus_tag="ACHY3M_003260"
     CDS             13108..14754
                     /gene="fumB"
                     /locus_tag="ACHY3M_003260"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS4318116.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            14877..15506
                     /gene="yjdF"
                     /locus_tag="ACHY3M_003261"
     CDS             14877..15506
                     /gene="yjdF"
                     /locus_tag="ACHY3M_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFS4318117.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(15645..17066)
                     /gene="melB"
                     /locus_tag="ACHY3M_003262"
     CDS             complement(15645..17066)
                     /gene="melB"
                     /locus_tag="ACHY3M_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFS4318118.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17169..18524)
                     /gene="melA"
                     /locus_tag="ACHY3M_003263"
     CDS             complement(17169..18524)
                     /gene="melA"
                     /locus_tag="ACHY3M_003263"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4318119.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            18807..19715
                     /gene="melR"
                     /locus_tag="ACHY3M_003264"
     CDS             18807..19715
                     /gene="melR"
                     /locus_tag="ACHY3M_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFS4318120.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            19914..22181
                     /gene="adiA"
                     /locus_tag="ACHY3M_003265"
     CDS             19914..22181
                     /gene="adiA"
                     /locus_tag="ACHY3M_003265"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFS4318121.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         6103 a         5515 c         5233 g         5484 t
ORIGIN      
        1 gatcgtcttt atcatgaagg atatgtattt tctgatatca ataccttgtc tcgttataca
       61 ctacacggac taggaataac tggtgtacat actgaagaaa acctgctacc agctccttca
      121 tcgtccttaa ttaatatatt gaaagaacat tataatgaag atgaaattag tgcgaaatta
      181 ccactagcat atgattacat tttaaataaa aaagaatcaa gctctattcc tgttgaaatt
      241 ttgaacaaac tttcagagtt accaccacat gaactactca cacctgttct tggccagagt
      301 gttaatcctc tgggcatggg ctactcatcc gataatggaa aaatcacaga gcaagtaata
      361 gtcagtggag ctgatggatt tgataatccc atatctggac ttatatatac ctatcttgaa
      421 gatctatata acatccatgt aaggatgcga gaaggtacac taaattcaca gaatcttcgt
      481 cagcttctgg aaaactctgt ttcttcatgc tttttgactg agcaaagtat taataaatta
      541 cttagtgagg cagaaaaaag gccttatcag tctttaacag aaatacatca gcatctcaca
      601 ggattaccaa ctattgccga tgcaaccctt tcattacttt ctgttggatt acctggtacg
      661 ggaaaactat tgcgcaggga gcaggactat gggcgtccac cagttacagc aattcaggat
      721 tccacatttg tactccctta taatttcaaa ggtattggtt ttaacgataa cattatatcc
      781 tctgcacctg tagcctcctc attacatttt atcgctgaac atgcgaaata tactttattg
      841 tcatggcctg agttttatcg tcatcatgca cagcgatggt tcgaaatggc taaaggatat
      901 ggaagccaga atattgattt tcaccctcag tctctattgg taacccaaga aggacgctgt
      961 atgggattag ccttacttta tttacagact gaagatactg ctcattatag cattctccag
     1021 gaaaacctaa tgactgtgag tgcacttcat cagaccagta atcgcgataa gttgccactg
     1081 tccaaagatg ataattcctt aatgacaaga acttatagtc tgattgaaat gctacagtat
     1141 cagggaaaca aatatattac caacgaatcg ctactacata agaccgcatg gaaccaagaa
     1201 agaataactt tattattcaa tgaaaaagga gttaagcgag ccctgataag cacgcctaat
     1261 catactctgg ttctgcaaca actggaggat atttaccggc tcactgatcc aaattttggg
     1321 catgcagatt tcctttcacc tatagatgct ctgaagttta ttgaggctat gatacaatta
     1381 actccaacac ttcaggaata ttatggccta ttaaacaaag acattaataa acatatacaa
     1441 gtacattatg ccgaatcaga tatggtctgg aataagcttc tgccagaaaa tgatgctgga
     1501 ctgagcacca gaattcagca caccaccacc gaccgtctgg cgaatctggc tgaaccagtc
     1561 gctgttgcag gtatctccct gccagtaaaa acactttatg atatcggagc cacccttgac
     1621 ggtcggcgca tcacctctcc tccaacatcg gagcaaatcc cttctctgcg tctcaacggt
     1681 gatgttctga atgattatct gtcccgcaca gttctgactc cagaacaggc tgataacata
     1741 agaaaaatac tgcacactca gggaatacgc agcggtaccc gtcccataga tccggagatg
     1801 attcgtggga cgcaggatga cctagtttcg tcacagactc gtctgcaaag gcaagcaaca
     1861 cgggttaaac agcaactcgc cggtgtactt gatactctgc aacagcactt ccagaacatt
     1921 ccacgttcat ccggtcgtca tctttctgta gagaatattg agctggctga tatcggaagt
     1981 gggcgtttca accttcaaat tcgagatgga gaaacattgc ataccacttc tgtggaagta
     2041 ccggaagttg tgtcccgttt tcagaaactt tctaccatgc tttcagccct gcctgccagt
     2101 ggaatcatgg atttcgacct cggcatgagt gtggtcggag tcgtccagta tgcccgcctg
     2161 ctacagcagg ggcacgaaga cagtacccta gccaagataa atctagctat ggatatcaag
     2221 caactttccg aagcaactct cggcagcatg attcagattg ccgggaataa gtttctcaat
     2281 acagaaggaa tccaggggtt cagactggaa agcgccgtcg ctgaaggtat gcgctcagta
     2341 gcaacccgta ccggaggcac aatggggaaa gccctttctg ccagtgcccg tgttcttgaa
     2401 ctgcctgtac tggaaacagt tctggggaca tggaacctgt acaacagcgt cattcagctc
     2461 cagcaagcta catcttattc tgagacaatg gctgcccggg tacagattgc gtttgattcc
     2521 atttctctgg gattaactgc cgcttcggta gctttcccgc cactaattat tgccactggc
     2581 cccattgcgg ctattggtat gggagcttcc agtattgcac gtaatgtggc acggaaagaa
     2641 gaacggcata cacaatggct ggaatataaa aaattcctga ctgatggcag taaacacatt
     2701 gttgtggcct ctccggaaag aggtctgctg gatttctccg gaaacaaagt ttttggaaaa
     2761 atggtgctgg atctgcgtca gtctcctcct ctcttgcatg gagaaagctc ttttaacgct
     2821 gaccgcaaaa tcggtcatcg tccggatctg ggggactggc aaattcgtga gaaggtgggg
     2881 tatgccaaca gtatcagtcc ctactcttct ctggcgcacg gttacgccaa cagtaaatgg
     2941 ccacgaacaa taccgaaaat tccctcggga gaatatgaca ccataattct gggctacggt
     3001 caccagtatc aggccaatac ggaaatagaa tatctgtcta actggattgt atggcgggaa
     3061 gccgtaccag acagtacttc ccgccacaaa cgtcctcctc tggaggttct taatagtcag
     3121 tgtactgtga tagctggaga gcgtaaaacc acagtacttc ccctgagagt gctcagcgat
     3181 ctgacaccgg aatgcacaga acaggctata tcgttaaaag attataaatt catactgaga
     3241 gggggaagcg gtgggctggc tgttcaggtc ggtggcgcgg gatattatga tattgatgca
     3301 aatcctgtgg caaaagaaaa tacgctctct tttcgcgggc taccggaaga gtttccgctc
     3361 acctttgatt tatcaaaaca aacacagtcg gtcatgctga aaacgccaga cgatgaggtg
     3421 ccggtaatga ccattaccca gaagggaata aacaccctgg taggtacagc cgctggtaaa
     3481 gaccgactaa tcggtaacga taaggacaat accttccata caagctctgg cggcggtaca
     3541 gtcatctccg gaggcgggaa taaccgctat attatccccc gggatttaaa aacgccgttg
     3601 acactgacgc tgtccagtaa ctcagtctct cacgaaatct ttctgccaga aacaacccta
     3661 gctgaattaa aacctgtcgc ctttgagctg agtttgattt actgggccgg gaacaacata
     3721 aatgttcaac cagaggatga agcaaaactg aaccactttg ccggaaactt cagggtgcat
     3781 acccgtgatg gcatgactct ggaggcggtt tcccgggaaa atggtattca actggcgatt
     3841 tcattatgtg atgttcaacg ctggcaggct gtttatccgg aagaaaataa cagaccggat
     3901 gccatactgg acaggctgca tgatatgggc tggagtctga caccagaagt ccggttccaa
     3961 ggaggagaaa cacaagtcag ctatgatccc ctgactcgtc agctcgttta ccagcttcag
     4021 gcgcgttact ctgaattcca gttggccggt agtcgccacc ataccacggc tgtaaccgga
     4081 actccgggaa gccgatacat tatcatgaag ccagttacaa cacagatatt accgacacaa
     4141 atcatactgg ctggtgataa tgaccatccg gaaacgattg atttactgga agctagtcct
     4201 gttctggttg aagggaaaaa agacaaaaac agcgtgatat taacgattgc tacgattcag
     4261 tattcccttc aactgacaat atccgggatc gaagaatcgc tgcccgagac aacccgtgtg
     4321 gcaattcagc ctcaggatac ccgtttactg ggtgacgtac tccggatctt accagataat
     4381 ggtaactggg tggggatttt ccggagtggt catacaccaa cggtaaaccg gctggaaaat
     4441 ttgatggcac tgaatcaggt aatgacgttc ctgccccggg tatccggaag tgcagagcag
     4501 gtattatgcc tcgaaaacct aggtggcgta aggaaaaaag tggaggggga gttactgtca
     4561 gggaagctga aaggtgcgtg gaaagccgaa ggtgaaccta ctgttccggt aaatatctca
     4621 gatctaagta tcccacccta ttcacgtctg tatctgattt ttgaagggaa aaataatgtg
     4681 ttgctacgca gtaaagtaca tgcagctccg ttgaaaataa catccgcagg agagatgcag
     4741 ttatctgaaa ggcagtggca acagcaggaa catattattg tcaagcccga caacgaagcc
     4801 ccctcattaa tactcagtga atttcgtcgt ttcactattt catcggataa aacattttct
     4861 ttaaaactga tgtgccatca gggtatggtc cgcatcgacc gcagatcatt atcggtcaga
     4921 ttgttctatc tgcgtgaaca gccaggtatc ggcagtttac gtctgacgtt cagagatttt
     4981 ttcacagaag tgatggatac aacggacagg gaaattctgg agaaagagct gagaccaatt
     5041 ctgataggag atacacaccg ctttatcaac gctgcataca aaaatcatct gaatatccag
     5101 ttaggagatg gcgttctgaa tctggcagac attgttgcgg aatacgcccg tattcaaaag
     5161 gaagaaacat caaaaatatt gtatcaatat caaggtgcca tgaaaaaaaa aacagatgga
     5221 ccatctgtgg tagaagatgc cattatgacc actactgtca caacagattc aggtgaacta
     5281 ttccctacct tccacccgtg gtatacagat gatttatcag ggcgttataa gagcgtacct
     5341 atggcaagaa aagcagatac tttgtatcac ctgacaccaa aaggtgatct acagataata
     5401 tatcaggtag ctacaaaaat ggtgaatcag gcgatgattg tatccctgcc aaactaccga
     5461 cacgagtggg aaaaatataa tttaagcatc ttatccgaaa tccctcagaa caataatact
     5521 gttgtacatt caatcctcag ggttaatggc cccacaatgc aggtgcgcac aattgactac
     5581 agaggaacgg atgaaaacaa tcccatagta tctttttcag atacaacctt catcaatggt
     5641 gaacagatgt tgagttatga ctcgcattca tcagggcgag tctattccag agaagaatat
     5701 atgatgtggg aattgcagca acgggtatca gaagcttcca gtgcccggac acaggattac
     5761 tggctgatgg atgcagcggt aagaaacgga gaatggaaga tcacaccaga attattacgt
     5821 cacacaccgg gatatatccg gagtacggta tcgaaatggt ccagaggatg gctgaaaacc
     5881 ggcacaatac tccagactcc agaagacaga aatacggatg tatacctgac taccatacag
     5941 aacaatgtat ttagtcgtca ggggggcggc taccaagtgt attatcggat tgatggaatg
     6001 gctggtgcgg atatagcgga taatgcacca ggggaaaccc gctgcaccct caggcccgga
     6061 acatgttttg aagtgacaag tgtggatgaa aggcattatg agtggaatat catttatgtc
     6121 acgctgaaaa cctgtggctg gagccgaaat ggccaaagca aaacgccgaa tggtgacaac
     6181 ctttttaact aattaaaatc tctgttaaag atgtataacc cggcatctat gtgctcacca
     6241 gcatatagat gcaatgtgat tcccctgatg gtcgtatcag agatggtatg cgagcctgta
     6301 catagatttg tgtaattgcc tgattttgat atgttcaatc caacatcaaa agcaggttaa
     6361 tttatggacg aaaaacagtt gcaggctctg gctaacgaac tggccaaaaa tctcaaaacc
     6421 cctgaagatc tcagccagtt cgatcgcctg ctgaagaaaa tcagcgttga ggcagctctc
     6481 aacgctgaaa tgtcccacca tctgggctac gataatttct gttcgccata atcctgagtt
     6541 cacaatgatg gaactctaca tggcgtatgc ggattaccac gatttgattg aactgacaga
     6601 gtccctgttc cgcaccctgg cacaagaggt tctgggtacc actaaagtca cttatggcga
     6661 gcatgtgttt gatttcggca aaccgtttga aaaactcacc atgcgcgaag caatcaaaaa
     6721 atatcgtcca gaaaccgata tggccgacct ggataatttt gatgctgcta aagcattagc
     6781 tgaatctatc ggtattacgg tagagaaaag ctgggggctg ggacgtattg tcacggagat
     6841 ctttgatgaa gtggcagaag cacatctgat tcagccaacc tttattacgg aatatccggc
     6901 agaagtgtca ccgctggcac gccgtaatga tgttaaccca gaaatcaccg accgttttga
     6961 attcttcatc ggtggtcgtg aaatcggtaa tggttttagc gaattaaacg acgcagaaga
     7021 tcaggctgaa cgtttccagg aacaggttaa cgctaaagct gcaggtgacg acgaagccat
     7081 gttctatgac gaagattacg tgactgcgct ggaatatggt ctgccgccaa ccgctggtct
     7141 gggtattggt atcgaccgaa tgattatgct gtttacaaac agtcatacta ttcgcgacgt
     7201 tattctcttc ccggcgatgc gcccacagaa ataaatttca ctttaatgaa cgaagcagtc
     7261 aggcgactgc ttcgttcgtc gttccgcaaa tccagttttg aggagagacg cttcgtttca
     7321 cgataaccag cgggtccact caactacagc tcctaaaaga gagaaaaaag taatgccaca
     7381 ggcagactca ttggccaggt tattccgacc aggaatgcac taagtaaacg aatatgtgtt
     7441 ttctcatgtg agataaacca gataatgaca aaggatatgt tcacaccaat cacataaaaa
     7501 attaatattt tagagaatag tgccattttt tatgttcttc ttatccttcc tggctacttg
     7561 taaaactgac ggaaatagtc atcaactgtc ataatgttga ggtcaaaatc aatatccggt
     7621 aagtctaagc atttaagcct gattaaatct tcgatttcac ttgcggctaa cgatgtcgtt
     7681 attgcgaagg aattttcatt caattgtcgt ggtatcccat attcatcagc cacgatagaa
     7741 gagaaattca tatcatacat caatgatctt aaccttaaga acactgagga ataagatgat
     7801 ggataatcaa aagaaacaac ataagtattg aacttgtttt taccttccat aatatgactc
     7861 ctatcaatat tgaatgctct ggggacgcaa aaattattaa ccccgattga tgattctcat
     7921 ataggagata ttcttttttt tgctcagtaa aggggatgac ttgggacaaa aaaacgcgct
     7981 ctctgcccat tctttgtgca tccccctacc cttcagatgc tctcctgcat cactccgcgg
     8041 agagcatgaa aaatatcgtg actctgtact ctcccatcaa ctgcgaatat catcatactc
     8101 ccgcgtttta tcaaattcgt gtttcgcaaa tgggcatagt gggataattt ttcgtttttc
     8161 ccgacgcatt ttttccacga ctttcgcaac cagctgttta ccaatcccct gccctttcag
     8221 gctttcatcg acatcggtat gttcgataat cgctaaattc tctccggtcg gcacaaagac
     8281 aatttcagcg atttgcttgc cttgttcgtc attaatgtaa aatttattgt ggccttcgcg
     8341 tatttccatt ttaccctcgc ttatttatga cggtatttca gcgcgccgct cgggcacgta
     8401 tcaatcactt taaccacagt gacgacgtcg acttcatccg gcatgatcca cggctttcgt
     8461 ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg acatatcgca
     8521 gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccctc gtccagtagc
     8581 gcctgatcca tgactttccc tctctcatca gtatgattat ctctataagc atagccctgg
     8641 aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat atagccaatc
     8701 attcttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa atttgtcaca
     8761 caaggaagct gatgagacat tcattgccct accgcatgtt acgcaaacgt ccgatgaaat
     8821 tgagtaccac agtgatctta atggtcagcg cggtactgtt ctcggtgcta ttggtggtgc
     8881 atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc aacaaggcac
     8941 tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg cagaaaaaac
     9001 cgcaggagag cggaatccag gccatcgcgg aagccgtgcg caaacgcaac gatctgctgt
     9061 ttattgtcgt taccgatatg caaagtcttc gctactcgca tcctgaagcc cagcgtattg
     9121 gtcagccatt taaaggtgat gacatcctta aagcgctgaa tggcaaagaa aatgtcgcta
     9181 tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac gatgaaaatc
     9241 ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc caacaaatca
     9301 atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc ggactgattg
     9361 gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa ccctacgaaa
     9421 tctccacgct gtttgagcag cgccaggcca tgttgcagtc tatcaaagaa ggcgtcgttg
     9481 ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa ttgctgaatt
     9541 accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg tcacaggtgg
     9601 tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag attacgatta
     9661 aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt atcatcggtg
     9721 ccatttcaac cttcagggac aaaactgaag tacgtaaact gatgcagcga ctcgacggtc
     9781 tggtcaacta tgctgatgca cttcgtgaac gatcccacga atttatgaat aaattgcatg
     9841 tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac attctcaaaa
     9901 cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa tctccggtta
     9961 tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat acgctgattt
    10021 taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg acgctgatta
    10081 ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa cccggtggcg
    10141 aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt aacgatgatg
    10201 gaccgggaat cgcacccgac aaaatcgatc acatttttga caaaggtgtc tcgacaaaag
    10261 gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat ctcggcggca
    10321 gcatcgccgt ggaatcggaa cccgggattt tcacacaatt ttttgtccag ataccctggg
    10381 acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg caatggtcgc
    10441 ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg gaacagcctc
    10501 gacgctggag aaagccaaag agattatctt caatagcgat acgcctatcg acctgatatt
    10561 gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc tgcataacgc
    10621 gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa ccattaaaga
    10681 ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt cccgctttga
    10741 agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc agtattatga
    10801 ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg atcctcgtcg
    10861 cttgccaaaa ggcttaacgc cgcagacttt acgcacgctg tgccagtgga ttgacgcgca
    10921 tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct cgcgtgtttc
    10981 ctgccgtaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca gtatccatta
    11041 tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact actcactact
    11101 gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta ttaccgtaat
    11161 gcaaaatacc cctggcacaa gttacatcct tcatcagtaa aacttgtgcc agatcaaata
    11221 taattatccc tccatcatcg ctaaaaatta atatctcttc aggtgaacgg tgtttttaat
    11281 ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt gtatttttac
    11341 ccacaaatgg gtagatcaga ttaatcttta aacctaatga tatctgccct gagaacaaaa
    11401 aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc tgcgcagtac
    11461 aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta tcacagtgca
    11521 taaatttgcc gctgctttaa tcagccaata tttactgtga ggtatttgct aaagccggta
    11581 acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca gaacgtgact
    11641 gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca tgttatttac
    11701 tatccaactt atcataatac tgatatgtct gttttatggt gccagaaagg gtggtatcgc
    11761 gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc accttcagcc
    11821 aggtaaacca ccggttgatg tcatgctggt tatcattgcg gtggtggcgg catcggcgac
    11881 cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc tgctgcgccg
    11941 caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga ccattctttg
    12001 cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg ccattaagaa
    12061 caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga tggggattat
    12121 cgccagtccg gtgtcggttg cggtcgtgtc tctggttgca atgctgggta atgtcacctt
    12181 tgatggtcgc catcttgagt tcctcgacct gctggcaatc accattccat cgacgttaat
    12241 cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg ataaagacga
    12301 agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg gtgataccgc
    12361 gacgctgctc gataaaaaac tgccgaaaag caactggctg gcaatgtgga ttttcctcgg
    12421 tgcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat ccttcggcgg
    12481 caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg gggcgctgat
    12541 tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct tccgttccgg
    12601 tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca tgttcggtgc
    12661 gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc cgtgggcgta
    12721 tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg cgctggcggc
    12781 gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg cttcagcacc
    12841 ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag cgattcagtt
    12901 tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt ttattctgcc
    12961 ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg cgatgtacgg
    13021 gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat tttcgaataa
    13081 caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta ccaggcacct
    13141 ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta cgttagcgtt
    13201 gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac cctactggcg
    13261 cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa acaggttgcg
    13321 gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcgct gcaattctta
    13381 agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac cggcaccgcg
    13441 atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga agaagcgctg
    13501 tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctattcaca gaatgcggcg
    13561 ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat cgacctgtac
    13621 gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg ctctgccaac
    13681 aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact gaaaaacttc
    13741 ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca tatcgcgttt
    13801 gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaagttagc aagcgctcac
    13861 tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg cgatgtccag
    13921 ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca gtttggcggt
    13981 aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc atcctgcccg
    14041 gtcggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat caaccgcgaa
    14101 ggtatctgga tcgaaaaact ggaacacaac ccaggccagt acattccaca agaactgcgc
    14161 caggccggtg aaggcgaagc ggtgaaagtt gaccttaacc gcccgatgaa agagatcctc
    14221 gcccagcttt cgcaataccc ggtatccact cgtttgtcgc tcaccggcac cattatcgtg
    14281 ggccgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa agaacttccg
    14341 cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc tgccggttat
    14401 ccatcaggtt cacttggccc aaccaccgca ggccgtatgg actcctacgt ggatctgctg
    14461 caatcccacg gcggcagcat gatcatgctg gcgaaaggta accgcagtca gcaggttacc
    14521 gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc ggcggcggta
    14581 ctggcgcagc agagcatcaa gcatctggag tgcgtcgctt atccggagct gggtatggaa
    14641 gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga tgacaaaggt
    14701 aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa gtaacctctt
    14761 cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc ttttgcgatt
    14821 gtaatttcac gcttgctggt gaatagtcag tattttccct gacttgcgaa ctcaccatga
    14881 cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg ttaatcctga
    14941 tttataccgg catttcggcc catgacaaac tcacctggct gatggaagtg acaccggtga
    15001 ttattgtcgt gccgctactg cttgccaccg ctaaacgtta tccgttaacg ccgctgctct
    15061 atacgctcat tttctttcac gccatcatcc tgatggtcgg cgggcaatac acctacgcga
    15121 aagtccccat cggttttgag gtgcaggaat ggctggggtt gagccgtaat ccgtatgaca
    15181 agctggggca ttttttccag gggctggtgc ctgcactggt ggcacgagaa attctcgtgc
    15241 gcgggatgta cgtgcgcgga cgtaaaatgg tggcgttcct ggtctgctgc gtggcgctgg
    15301 cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc gctggcaatg ggccagggag
    15361 cggatgattt tctcggcacc cagggcgacc agtgggacac gcaatccgat atgttctgcg
    15421 cgctgcttgg cgcattaacg acggtgatat tcctcgctcg ctttcattgc cgccagctac
    15481 ggcgctttgg cttgataacc ggataacaac gtgcgctttg ttgatgccgg atgcggcgtg
    15541 aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag acgtggcctg
    15601 ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc tttcacatca
    15661 ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt gcgatattta
    15721 tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata gaaacggaag
    15781 tagagaatca gcgttaccat gaaaaacaga gttggtagag caatcataat aaactgcata
    15841 cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat ccctaacaca
    15901 accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt ctgcacggag
    15961 taagcgatac tttcacagcg tacgtgcagt ttatattcac cgtaatcaac ggtatctgcc
    16021 accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa aatacccgca
    16081 atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag aacaccacag
    16141 cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa tgatttaact
    16201 aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga cagataatag
    16261 gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa tccggtaata
    16321 atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag ctgatcgttt
    16381 ttataaatta gcgcaacgat ggctttaagt gtcagatggc ttccttcagc agacggttga
    16441 ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga aacaataaaa
    16501 aaggcgatta gtaccagagt gaacatctga aagccaaatc cccgatcgcc accgccgaca
    16561 taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact ggcaaaaaaa
    16621 cgcggataag gaaccagttg ttcgcgctca cgtttatcga gcgtgatggt tggaaccagc
    16681 gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta ggtcacgcaa
    16741 acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag aaataagatt
    16801 acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca tcgcgatcgc
    16861 gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat cctcgccacc
    16921 agaaacaaag taccgaccaa acccacagac agcccaacga catcggtgta gtaatacatg
    16981 aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa tccataactg
    17041 agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa cgcccgcctt
    17101 ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca gtagcgttta
    17161 gtcgcgtttt aacggtgcaa ccagcctggc agccagtcgc cgtgggcggc aatcaggtcg
    17221 tcaacaagag catatatttc atcaatgccc agcacggcag cagtatgcgg gtccatcatc
    17281 gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag cagcgtctgt
    17341 acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc gactttggtc
    17401 ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc ttgtggcagg
    17461 ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc gccagtccag
    17521 atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat ccgggaggcg
    17581 tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca gcgtttcggg
    17641 tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctgg cttaataaac
    17701 cacggtgtgt actcagcaaa atgttctgac gattccgtga cgaagtagcc cagctttttg
    17761 aacatttcgt agcgcacaat attctggcag cgagtattgc catgaatatt cggcttcggt
    17821 gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacata actgccgtcg
    17881 gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc gcaacggtaa
    17941 cgcagcgtag ctgggtcgat attgaggtca cgcgccagct cttccgccgt tccctgcacc
    18001 gaatggcaca gcccgacctg tttgatatgc ggatagcggg catacatcgc ccaggtattc
    18061 atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc cgtcatgtcc
    18121 tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc gcccggcccc
    18181 aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa atcagtcacc
    18241 gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc atcatgtaag
    18301 gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga atccataagc
    18361 ttacgcacca caatatgcga ctcttccagg cgggtgggat caatgtccat cagggcaata
    18421 tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt aacgaaaatc
    18481 gtcgaaccag cgccgataaa tgtaattttg ggtgcagtca tcatgcagat ctcctggctt
    18541 gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt ccaggttgag
    18601 cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta tggtaataag
    18661 ttacctgaaa agcagagttt tcggcctcat gatgctcgga cgctcccctc gcctctctgg
    18721 gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct gccataattc
    18781 tgatattcca ggaaagagag ccatccatga atacagatac gtttatgtgc agcagcgacg
    18841 aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg gagattgagt
    18901 ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa gtgaatgtgc
    18961 ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc aatcagggtc
    19021 atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga acctgtcaga
    19081 gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg gataaagacc
    19141 tgattaacca cgtcactcac ggcatggtga tcaaatcact ggccacacag caacttagcc
    19201 cgtttgaagt gcgccgctgg cagcaggaat taaacagtcc gaacgagcaa attcgccagc
    19261 tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctggc tgggaaccga
    19321 ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat gcgcaatttt
    19381 atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg accatcaacg
    19441 atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc cagcgggtca
    19501 tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt cgcgcgttac
    19561 tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt cgttcaagta
    19621 gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa taccgcaaac
    19681 ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat cagccaaaaa
    19741 aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac ataacgtatt
    19801 gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca gcccgaaaag
    19861 gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca atgatgaaag
    19921 tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac gccgttgagc
    19981 gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc tcctttgatg
    20041 atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc agctatcaaa
    20101 tggaacatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt catgagcgcc
    20161 aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc gcaatggacc
    20221 gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc gccgacttta
    20281 tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg ccgccactgt
    20341 tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg ccaggccacc
    20401 agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac tactatggtg
    20461 aaaatctgtt ccgcaccgac atgggcatcg aacgaacttc cctcggttct ttgcttgacc
    20521 atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt gccgatcgct
    20581 cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag gcttgcatga
    20641 ccgataacga tgtcgtggtc gttgaccgta attgccataa atccatcgaa caaggcttga
    20701 tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac ggcattatcg
    20761 ggccaatcta tccgcaggaa atgcaacctg aaaccttgca gaagaaaatc agtgaaagcc
    20821 cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtgacc aactgcacct
    20881 atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa acctccgatc
    20941 gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc tatgccgatc
    21001 actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc gccacccact
    21061 ccacccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta cgtgaaggtc
    21121 gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc accacctccc
    21181 cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac ggcaacagcg
    21241 gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag gcgatggcgc
    21301 ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg aacaaagaag
    21361 tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca accaaactgc
    21421 tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac ggcttcaaag
    21481 atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt gctccgggaa
    21541 tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc actgcctggc
    21601 ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg ttcctgttct
    21661 ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc tccttcaaac
    21721 gccactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt gaacaatatc
    21781 ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt gcctggctga
    21841 aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg atggcggaaa
    21901 tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg gtatccattg
    21961 aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca ggaatcccga
    22021 tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt tatttacgct
    22081 cgctgcaatc ctgggaccac catttccctg gatttgaaca cgaaactgaa gggactgaaa
    22141 ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc cgctgaaggc
    22201 gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat ggcaacgttt
    22261 tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac cccgcgcttt
    22321 tttttgcctg ttatt
//
LOCUS       DAZUFB010000075        22332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZUFB010000075 DAZUFB010000000
VERSION     DAZUFB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.075"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..851
                     /gene="yibB"
                     /locus_tag="ACHY3M_003266"
     CDS             <1..851
                     /gene="yibB"
                     /locus_tag="ACHY3M_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFS4318122.1"
                     /translation="STTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALE
                     NEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPR
                     QLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWD
                     FPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLES
                     QKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALR
                     IFLSRK"
     gene            1126..2322
                     /gene="kbl"
                     /locus_tag="ACHY3M_003267"
     CDS             1126..2322
                     /gene="kbl"
                     /locus_tag="ACHY3M_003267"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4318123.1"
                     /translation="MRGKFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQERIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2332..3357
                     /gene="tdh"
                     /locus_tag="ACHY3M_003268"
     CDS             2332..3357
                     /gene="tdh"
                     /locus_tag="ACHY3M_003268"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4318124.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3608..4642
                     /gene="waaH"
                     /locus_tag="ACHY3M_003269"
     CDS             3608..4642
                     /gene="waaH"
                     /locus_tag="ACHY3M_003269"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFS4318125.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(4629..5588)
                     /locus_tag="ACHY3M_003270"
     CDS             complement(4629..5588)
                     /locus_tag="ACHY3M_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFS4318126.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLNESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(5592..6851)
                     /gene="envC"
                     /locus_tag="ACHY3M_003271"
     CDS             complement(5592..6851)
                     /gene="envC"
                     /locus_tag="ACHY3M_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFS4318127.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(6885..8429)
                     /gene="gpmM"
                     /locus_tag="ACHY3M_003272"
                     /gene_synonym="pgmI"
     CDS             complement(6885..8429)
                     /gene="gpmM"
                     /locus_tag="ACHY3M_003272"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFS4318128.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            8674..9105
                     /locus_tag="ACHY3M_003273"
     CDS             8674..9105
                     /locus_tag="ACHY3M_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4318129.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            9247..9498
                     /gene="grxC"
                     /locus_tag="ACHY3M_003274"
     CDS             9247..9498
                     /gene="grxC"
                     /locus_tag="ACHY3M_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFS4318130.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            9561..10028
                     /gene="secB"
                     /locus_tag="ACHY3M_003275"
     CDS             9561..10028
                     /gene="secB"
                     /locus_tag="ACHY3M_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFS4318131.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            10028..11047
                     /gene="gpsA"
                     /locus_tag="ACHY3M_003276"
     CDS             10028..11047
                     /gene="gpsA"
                     /locus_tag="ACHY3M_003276"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4318132.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            11127..11948
                     /gene="cysE"
                     /locus_tag="ACHY3M_003277"
     CDS             11127..11948
                     /gene="cysE"
                     /locus_tag="ACHY3M_003277"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS4318133.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(12001..12474)
                     /gene="trmL"
                     /locus_tag="ACHY3M_003278"
     CDS             complement(12001..12474)
                     /gene="trmL"
                     /locus_tag="ACHY3M_003278"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4318134.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(12660..13850)
                     /gene="lldD"
                     /locus_tag="ACHY3M_003279"
     CDS             complement(12660..13850)
                     /gene="lldD"
                     /locus_tag="ACHY3M_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFS4318135.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            complement(13847..14623)
                     /gene="lldR"
                     /locus_tag="ACHY3M_003280"
     CDS             complement(13847..14623)
                     /gene="lldR"
                     /locus_tag="ACHY3M_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFS4318136.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            complement(14623..16278)
                     /gene="lldP"
                     /locus_tag="ACHY3M_003281"
     CDS             complement(14623..16278)
                     /gene="lldP"
                     /locus_tag="ACHY3M_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFS4318137.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(16647..21497)
                     /gene="ehaG"
                     /locus_tag="ACHY3M_003282"
     CDS             complement(16647..21497)
                     /gene="ehaG"
                     /locus_tag="ACHY3M_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFS4318138.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIVNAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            complement(21497..22239)
                     /locus_tag="ACHY3M_003283"
                     /pseudo
     CDS             complement(21497..22239)
                     /locus_tag="ACHY3M_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5239 a         5634 c         5767 g         5692 t
ORIGIN      
        1 catccaccac aattatcact gcatattttg acattggtag aggggattgg actgctaata
       61 aagggttccg tgaaaaacta gctcgttcag ttgatgttta ttttagttac tttgagcgtc
      121 tggccgcact tgaaaatgag atgattattt tcacttctcc cgatttgaaa ccaagggttg
      181 aagcgattcg caacggaaaa ccaacaacgg ttatcgttat cgatataaaa aagaaattta
      241 gatatatcag aagtcgtatc gaaaaaattc aaaaagatga gtcgtttaca aatagacttg
      301 agcctcgaca attaaaaaac ccagagtact ggtcaccaga gtatgttttg gtatgtaacc
      361 ttaaggccta ttttgtaaat aaagctatca acatgggctt agtgaaaacg cctcttgttg
      421 cgtggataga ttttggctat tgccgtaagc caaatgtaac tcgaggattg aaaatttggg
      481 atttcccatt tgatgaaagt aagatgcatc ttttcactat aaaaaaaggg cttactgtca
      541 catctcaaca acaggttttt gatttcatga ttggcaatca tgtatatatt attgggggcg
      601 caattgttgg ttcacagcat aaatggaaag aattctataa attagttctg gaaagtcaaa
      661 aaataacact aaataataac attgtggatg atgaccaggg catttttgtc atgtgctatt
      721 acaaacgccc cgatctcttc aacctaaact atctggggag aggaaaatgg ttcgatttgt
      781 ttcgctgctt caggagtaac actttagggg caaaaatgca ggcgctgaga attttccttt
      841 caagaaaata ggtttccctc cattagtggg tagcgttttg ataccaactg agataggaaa
      901 aatgatgctt tggcatcatt ttttcttttt atatcaatgc agtaaaatca atcatcatta
      961 tgcccagcca aaactgtaca gtttttctac tatctgtagg tgttgagaga catcaagtta
     1021 cacgttattt atcctgaatt ttgcagaagt gttaacgcgt catctcgtcg cgacctataa
     1081 gtttgggtaa tatgtgctgg aatttgccct gtctggagaa tcgcaatgcg tggaaaattt
     1141 tatcagcagt taaccaacga tctggaaacc gcacgggcgg aagggttgtt taaagaagag
     1201 cgcattatta cgtctgcgca gcaagcagat atcactgtgg ctgatggaag ccacgtcatt
     1261 aacttttgtg ccaacaacta tctcgggctg gcgaatcatc ctgatctgat tgcggcggca
     1321 aaggcgggaa tggattctca cggtttcggc atggcttcgg tgcgttttat ttgcggcact
     1381 caggacagcc ataaagagct tgaacaaaaa ctggcggcct tcctggggat ggaagatgcg
     1441 attctctact cttcctgctt tgatgctaac ggtggcctgt ttgaaacgct gctgggcgcg
     1501 gaagacgcca ttatctccga cgcactgaac cacgcgtcta ttattgatgg tgtgcgtctg
     1561 tgtaaagcta aacgctatcg ctatgccaac aacgatatgc aggagcttga agctcgtctg
     1621 aaagaagcgc gtgaagccgg tgcgcgtcat gtgctgattg ccaccgatgg tgtattctcg
     1681 atggacggcg tgattgccaa tctgaagggc gtttgcgatc tggcagataa atacgatgcc
     1741 ctggtgatgg tagacgactc ccacgcggtc ggttttgtcg gtgaaaatgg tcgtggttcc
     1801 catgaatact gcgatgtgat gggccgggtc gatattatca ccggtacgct tggtaaagcg
     1861 ctgggcgggg cttctggtgg ttataccgcg gcgcgcaaag aggtggttga gtggctgcgc
     1921 cagcgttctc gtccgtacct gttctccaac tcgctggcac cggccattgt tgccgcgtcc
     1981 atcaaagtac tggagatggt cgaagcgggc agcgaactgc gtgaccgtct gtgggcgaac
     2041 gcgcgccagt tccgtgagca aatgtcggcg gcgggcttta ccctggcggg agccgatcat
     2101 gccattattc cggtcatgct tggcgatgca gtagtggcgc agaaatttgc ccgtgagctg
     2161 caaaaagagg gcatttacgt caccggtttc ttctatccgg tcgttccgaa aggtcaggag
     2221 cgtattcgta cccagatgtc tgcggcgcat acccctgagc aaattacgcg tgcagtagaa
     2281 gcattcacgc gtattggtaa acaactgggc gttatcgcct gaggatgtga gatgaaagcg
     2341 ttatccaaac tgaaagcgga agagggcatc tggatgaccg acgttcctgt accggaactc
     2401 gggcataacg atctgctgat taaaatccgt aaaacagcca tctgcgggac tgacgttcac
     2461 atctataact gggatgagtg gtcgcaaaaa accatcccgg tgccgatggt cgtaggccat
     2521 gaatatgtcg gtgaagtggt aggtattggt caggaagtga aaggcttcaa aatcggcgat
     2581 cgcgtttctg gcgaaggcca tatcacctgc ggtcactgtc gcaactgtcg tggcggtcgt
     2641 acccatctgt gccgcaacac gatcggcgtc ggcgttaacc gtccgggctg cttcgccgaa
     2701 tatctggtga tcccggcgtt caacgccttc aaaatcccag acaatatctc cgacgacctg
     2761 gcttccattt ttgatccctt cggtaacgcc gtgcataccg cgctgtcgtt cgatctggtg
     2821 ggcgaagatg tgctggtttc tggtgcaggt ccgataggta ttatggctgc ggcggtggcg
     2881 aaacacgttg gtgcacgcaa tgtggtgatc accgatgtta acgaataccg ccttgagctg
     2941 gcgcgcaaaa tgggtatcac ccgtgcggtt aacgtcgcga aagaaaacct taatgatgtg
     3001 atggctgaac tgggcatgac cgaaggcttt gatgtcggtc tggaaatgtc cggcgcgccg
     3061 ccagcgtttc gtactatgct cgataccatg aaccacggtg gtcgtattgc gatgctgggt
     3121 attccaccgt ccgatatgtc tatcgactgg accaaagtaa tctttaaagg cttgttcatt
     3181 aaaggtattt acggtcgtga gatgtttgaa acctggtaca agatggcggc gctgattcag
     3241 tcaggtctgg atctctcgcc gatcattacc catcgtttct ctatcgatga tttccagaag
     3301 ggctttgacg ctatgcgttc gggccagtcc gggaaagtaa ttctgagttg ggattaacac
     3361 gaacaagggc tggtattcca gcccttttgc ctgaggataa tccgttaaat atgtaaaatc
     3421 ctgtcagtgt aataaagata tcgtaattgc gctgatctct tatatagctg ctctcattat
     3481 ctctctaccc tgaagtgact ctctcacctg taaaaataat tgtaaaaata atatctcaca
     3541 ggcttaatag tttcttaata caaagcctgt aaaacgtcag gataacttca gaggtcgtcg
     3601 gtaatttatg atgaacagca ccaataaact tagtgttatt attccgttat ataatgcggg
     3661 cgatgatttc cgcacttgta tggaatcttt aattacgcaa acctggactg ctctggaaat
     3721 cattattatt aacgatggtt caacggataa ttctgttgaa atagcaaagc attacgcaga
     3781 aaactatccg cacgttcgtt tgttgcatca ggcgaatgct ggcgcatcgg tagcgcgtaa
     3841 tcgtgggatc gaggtggcga cgggcaaata tgtcgctttt gtcgatgctg acgatgaggt
     3901 ctatcccacc atgtacgaaa cgctgatgac catggcgtta gaggatgacc tcgacgtggc
     3961 gcagtgcaac gctgactggt gttttcgtga aacgggagaa acctggcaat ccatccccag
     4021 cgatcgcctt cgctcaaccg gcgtattaac cggcccggac tggctgcgga tggggctttc
     4081 ttcgcgccgt tggactcacg ttgtctggat gggggtttat cgccgtgatg ttattgttaa
     4141 aaataacatt aaatttattg ccggattaca tcatcaggat attgtctgga caacagaatt
     4201 catgtttaac gcgctgcgtg cgcgatatac cgagcaatca ttatataaat attatctgca
     4261 taatacgtca gtgagtcggt tgcatagaca agggaataaa aaccttaatt atcaacgtca
     4321 ctatattaag attacccgcc tgctggagaa attaaatcga aattatgccg acaaaattac
     4381 gatttatccg gaatttcatc agcaaataac ttacgaagca ttgcgtgttt gccatgcggt
     4441 gcgcaaagag ccggatattc ttacccgcca acggatgatt gccgagatat ttacttccgg
     4501 tatgtataag cgcctgatta ccaatgtgcg cagcgtgaag gtcggttacc aggcgttact
     4561 gtggtctttc cgcttatggc aatggcgcga caaaacgcgg tcgcaccatc gcattacgcg
     4621 tagcgccttt aatttgcgct agcgttaaac ttcgccgctt cgggctgttt gccccagcct
     4681 tgccactgat gctggaagta aacgatcagc gtgctctggc tgatgctttc gtttaatact
     4741 tcaaagaagc gattagcata aacaggttct atcggtttct tcggtttgca cagcttcacg
     4801 ccacggaacg gattacgcgg tgcatcaggc acagcgtttt tcggcggtgt agaagtatca
     4861 acctgcggtt cattcagcaa gctgctggct ttcaccagcg taatgtctgg cggcaggtta
     4921 taaaccatct gttgtagcac gcgtaccgtt gaagggtgag gatgcccaat ggcaatggtt
     4981 gaaccgttgc gacgcgccag atcaattgcg cgattaaatt gcacacggat gtccgcttcg
     5041 ttctgcgaat cgtcgaggaa caccttccgt ttgatcacct tcacgccagt accttgcgcg
     5101 gcgcgcatcg cctgggtatt accgatggtt acgctgtcga ggaagtaaag attgtaacgc
     5161 tccagtgcct gcatcacttt ctgcatacca aacaggttag aggtcatctt gctacccatg
     5221 tggttgttga tccccacggc atagggcacg ttattgaccg cactacgaat aatgcgctca
     5281 atttcgtcgc tgctcatctc cgggcgcagc gtatttttct ccagcggctg tttactcagt
     5341 ggtgccatcg ggagatgaat caacacttcg tgcccgctgt tatgcgcttt ggtcgccatc
     5401 tctctggcgt gcggtgaatc gggtaataca gcgacggaga tagcggaggg catcgccagc
     5461 acctggtttt cgttgtgcgg gcgataccca aaatcatcaa tgacgatggc aagtttgcca
     5521 gcaagtacgg gggaggagag cgccaacaga gcggcaaatg caagaacgtt acgacgaaat
     5581 ggaaacaaaa cttatcttcc caaccacggc tgtggattga ccgcctgacc ctggcggcga
     5641 atttcgaaat agagtgaagg ccgaccctga ccgccactgc tgcccaccag tgcaattggc
     5701 tggcccgcgc gaacctgcga accaacgctc accagtgcgc tctgattata gccgtaaaga
     5761 ctcatgtcgc ctttaccgtg ctcaaccacc accaccagac cgtaaccttg cagccagtca
     5821 gccagaatca cccgaccgtc ggcaatcgct ttaacttcag taccttcaga ggcaccgata
     5881 accattcctt tccagcgtag ttcgccctgt agctgttcac catagcgatg cagcgtcggc
     5941 ccgcgaaccg gccagaatgc ctgaccacgc ggcgcaccca ggccaccagt tcgggacatc
     6001 agcgattttt cgctttcggt cggtttatag gtggtgcctt tgcgcgtcgc ttctttctgg
     6061 cggtcgcgaa ccgcctgggc ctcacgcgct tctcgttcag cacgcgcttt cgccgcggct
     6121 tccgcacggg caatgctgtt acgcagacgg gattcgttgg cacgcagttc acttagctgc
     6181 tgctgacctt gctggatgga agattccagc cccgccagcg tctttttacg ctcgttcaga
     6241 gcctgagtca gtttcgcctg ttgggcgcgc tgttcatata acagcgtttg ttgctcgctt
     6301 tgtttctctt ccagttcagc acgctgcatg gcgacttctt cacgcgtttg cttcaactgg
     6361 gcaatggttt cttgtcgcgc ctggttgaga tagccgaaat aagcctgtaa acgctgtcca
     6421 cgctggcttt cttcaccgct gagaattagc tgaataccgg tatgctcgcc ctgacggaat
     6481 gcggcatcca gttgcgcggc gaggctgcgc tcctgggcgg ctttttgctg ctccagtttg
     6541 gcaatcgacg cgttcatctc atcaatctgt ttattgagtt gattgagcgt gttttgcgtt
     6601 tcgcgcagct tacgggtggc ttcagagatc gcttcttcct gctttttcaa ttgtgcgagc
     6661 aggcttgcgc gttgttgttg cttttggcgt accgcgcgct cttttgcggc gatatcggcc
     6721 tgaatagatt tgagttggtc acgctcatcc gcgtgggcgg aaaaggcgca caacaatacg
     6781 ccagcgctaa gaacgctggc gtagatgatg ggcctgattg caaacctgcg cggtttcacg
     6841 gcccgtgtca tggtattaat cgcctttccc ctcatgggga gggattattc cacgatgaac
     6901 agcggcttac cagtcatctc ttgcgggatt tccataccca tcagcgacaa catggtcggc
     6961 gcgatgtcag aaagtttgcc gccttcaacc gctttcacgt tcttatcacc aacataaatc
     7021 agcggaactg gcaggttggt gtgtgccgtg tgtgcctgac ccgttgccgg atcgcgcatc
     7081 tgttcagcgt taccgtggtc agcggtgatc agcagttgtc cacccacgga ctcaaccgct
     7141 ttcgcgactt cttccacgca gtgatccagt gcttcaaccg ctttaaccgc cgcttccatc
     7201 accccggtgt gacctaccat gtcgccgttc ggatagttac agataatggt gtcgtatttg
     7261 ccgctcttga tggccgcaac cagtttttcg gtcagctctg cggagctcat ttccggttgc
     7321 agatcgtagg tagccacttt cggtgagttg atcagaatgc gatcttcgcc tttgaacgac
     7381 tcttctacgc caccgttgaa gaagaaagta acgtgggcat atttttcggt ttcggaaata
     7441 cgcaactgag ttttgtcgtt tttcgccatc cactcgccga aggtgttaac cagggaagcg
     7501 ggtgggtaag caaccgcagt tttgatgtca gcggcgtatt cggtcagcat cacgaaatcg
     7561 acgttaacca ctttcttacg cgcgaagcca tcgaaatcag cgttcacgaa tgcacgagtg
     7621 atttcacgcg cgcggtcagc acggaagttc atgaaaatca gcgcatcgcc gtcttccatt
     7681 gccgcatctg gctgaccttc tgcacgaata acggtcgctt tcacgaactc gtcgttttcg
     7741 tcgcgagcat aagctgcctg caaaccagca acggcagtat cggcctggaa ctcaccctgc
     7801 gccagagtca gcaggtcata ggctttttca acgcgatccc aacggttatc gcggtccatc
     7861 gcgtagtaac gaccaatgat ggacgctacg cggcctttac ccagcgcagc aaatttctct
     7921 tcaaattttt tcagcgagga ttcagcactg cgcggcgggg tgtcgcgacc gtcaaggaat
     7981 gcgtgcaggt agattttttc tgcgccgcgt tcagctgcca gttctaccat cgccatgatg
     8041 tgatcttcgt ggctgtgtac gccgcctgcg gagagcagac ccataatgtg tactgcttta
     8101 cctgcgtttt tcgctttatc taccgcgcca gtcagcactg gattagcaaa gaaagcgcga
     8161 tctttgattt caacgtccag acgagtcagg tcctgataca cgatgcggcc ggcacccagg
     8221 ttaacgtggc ctacttcgga gttacccatc tgacggtcag gcagaccgac ttccagaccg
     8281 gaagcgtcga tcagggtatg cggacgattg gcccacagtg catccattac cggggtttta
     8341 gcactaaaaa tggcgttatc ctgctgttct tcacgatagc catagccatc cagaatcacc
     8401 agtaccatag gttttttaga aaccgacatt gcgacaacct catactcaag agtcaaaatt
     8461 tgcgtaattt tactacagct gaatcgataa aatagcctct gaagatcaaa atatacgacc
     8521 gctcgcggca agccgatgcc agatttgcgc ttttttttcg tcgtgccccg caaaattgca
     8581 ttccagttaa cgcgctggct gtatttgccg caccgcgcag gtatactcct ttcctggttt
     8641 ttttaatcac tacgtcggga gttgttaccc cccatgcaag aaattatgca atttgttggc
     8701 cgtcatccca tactgagtat cgcctggatc gcgttactgg tggcggttct tgtgactacg
     8761 tttaagagcc tgacctcgaa agtgaaggtg attactcgtg gtgaagctac gcgtctgatc
     8821 aacaaagaag acgctgtggt tgtggattta cgtcagcgtg atgacttccg taaaggccat
     8881 atcgcaggtt ctatcaatct gttgccgagt gaaatcaaag ccaacaatgt tggtgagctt
     8941 gagaagcaca aagacaaacc ggttatcgtg gtagacggtt ctggcatgca gtgccaggag
     9001 cctgcaaacg cgctgacgaa agctggtttt gcgcaagtat tcgtgctgaa agagggcgtt
     9061 gctggctggg ctggcgaaaa cttgcctctg gtgcgcggca aataatttac ctgtcgtctt
     9121 tcatgatgca ggccgttagc tgcaattagg cccccagcga cttattgtta taagcttctg
     9181 gggacactcc attttgcagc cgccctgcaa catgaaattt cagggtaaaa aaacaggagc
     9241 taaattatgg ccaatgttga aatctatacc aaagaaacct gcccgtattg ccatcgtgca
     9301 aaagcactgc tgagcagcaa gggcgtgagt tttcaggagt tgccgatcga tggcaatgcc
     9361 gccaagcgtg aagagatgat caaacgcagc ggtcgcacca cggttcccca gatttttatt
     9421 gacgcacagc acattggcgg ctgtgatgac ttgtatgcat tggatgcacg tggtggactg
     9481 gatcccctgc tgaaataacg tgtgaacgtt ggcattacat tgcgcagtat ttaaggacaa
     9541 cacttaaggg ttttctacac atgtcagaac aaaacaacac tgaaatgact ttccagatcc
     9601 aacgtattta taccaaggat atctctttcg aagcgccgaa cgcgccgcac gttttccaga
     9661 aagattggca accagaagtt aaacttgatc tggatacggc atcttcccaa ctggcagatg
     9721 acgtatacga agtggtactg cgtgttaccg taacggcctc cctgggcgaa gaaaccgcgt
     9781 tcctgtgtga agttcagcag ggcggtattt tctccatcgc gggtatcgaa ggcacccaga
     9841 tggcacattg cctgggcgca tactgcccga acattctgtt cccgtatgct cgtgagtgca
     9901 tcaccagcat ggtatcccgc ggtacattcc cgcaactgaa ccttgcgccg gttaacttcg
     9961 atgcgctgtt catgaactat ttgcagcagc aggctggcga aggtactgaa gaacatcagg
    10021 atgcctgatg aaccaacgta atgcttcaat gactgtgatc ggtgccggct cgtacggcac
    10081 cgctcttgcc attaccctgg caagaaatgg ccacgaggtt gtcctctggg gccatgaccc
    10141 tgaacatatc gcaacgcttg aacgcgaccg ctgtaacgcc gcgtttctcc ccgatgtgcc
    10201 ttttcccgat acgctccatc ttgaaagcga tctcgccact gcgctggcag ccagccgtaa
    10261 tattctcgtc gtcgtaccca gccatgtctt tggtgaagtg ctgcgccaga ttaaaccgct
    10321 gatgcgtcct gatgcgcgtc tggtgtgggc gaccaaaggg ctggaagcgg aaaccggacg
    10381 tctgttacag gacgtggcgc gtgaggcctt aggcgatcaa attccgctgg cggttatctc
    10441 tggcccaacg tttgcgaaag aactggcggc aggtttaccg acggctattt cgttggcctc
    10501 taccgaccag acctttgccg atgatctcca acaattgctg cactgtggca aaagtttccg
    10561 cgtttacagc aacccggatt tcattggcgt gcagcttggc ggcgcggtga aaaacgtcat
    10621 tgccattggc gcggggatgt ctgacggtat cggttttggt gcgaatgcgc gtacggcgct
    10681 gatcacccgt gggctggctg aaatgtcgcg tcttggtgcg gcgctgggtg ccgatcctgc
    10741 cacctttatg ggcatggcgg ggctgggcga tctggtgctt acctgtaccg acaaccagtc
    10801 gcgtaaccgc cgttttggca tgatgctcgg tcagggcatg gatgtacaaa gcgcgcagga
    10861 gaagattggt caggtggtgg aaggctaccg caatacgaaa gaagtccgcg aactggcgca
    10921 tcgcttcggc gttgaaatgc caataaccga ggaaatttat caagtattat attgcggaaa
    10981 aaacgcgcgc gaggcagcat tgactttact aggtcgtgca cgcaaggacg agcgcagcag
    11041 ccactaaccc cagggaacct ttgttaccgc tatgacccgg cccgcgcaga acgggccggt
    11101 cattatctca tcgtgtggag taagcaatgt cgtgtgaaga actggaaatt gtctggaaca
    11161 atattaaagc cgaagccaga acgctggcgg actgtgagcc aatgctggcc agtttttacc
    11221 acgcgacgct actcaagcac gaaaaccttg gcagtgccct gagctacatg ctggcgaaca
    11281 agctgtcatc gccaattatg cctgctattg ctatccgtga agtggttgaa gaagcctacg
    11341 ccgctgaccc ggaaatgatc gcctctgcgg cctgtgatat tcaggcggtg cgtacccgcg
    11401 acccggcagt cgataaatac tcaaccccgt tgttatacct gaagggtttt catgccttgc
    11461 aggcctatcg catcggtcac tggttgtgga atcaggggcg tcgcgcactg gcaatctttc
    11521 tgcaaaacca ggtttctgtg acgttccagg tcgatattca cccggcagca aaaattggtc
    11581 gcggtatcat gctcgaccac gccacgggca tcgtcgttgg tgaaacggcg gtaattgaaa
    11641 acgacgtatc gattctgcaa tctgtgacgc ttggcggtac gggtaaatct ggtggtgacc
    11701 gtcacccgaa aattcgtgaa ggtgtgatga ttggcgcggg cgcgaaaatt ctcggcaata
    11761 ttgaagttgg tcgcggcgcg aagattggcg caggttccgt ggtgctgcaa ccggtgccgc
    11821 cgcatactac cgccgctggt gttccggctc gtattgtcgg taaaccagac agcgataagc
    11881 catcaatgga tatggaccag catttcaacg gtattaacca tacatttgag tatggggatg
    11941 ggatctgatg tgctgtgatc gtgccggatg cgatgtaatc atctatccgg catacagtaa
    12001 ctaatctctc aacaacgctc ccggatatcc cagctgccgc caggcttcat acaccactac
    12061 cgacaccgca ttggacaggt tcatgctgcg gctgtccggc accattggaa tgcgaatttt
    12121 ttgttcagcg ggcagggcat caagaatgct cgctggcagg ccgcgtgttt ccgggccgaa
    12181 catcagataa tcgccatcct gatagtttac ggcgctgtga gcaggcgtac ctttcgtagt
    12241 gagggcaaac aggcgctggg gattttctgc ttcgaggaac gcgcgaaagt catgatgacg
    12301 cgtaacggcg gtaaactcgt gatagtccag ccccgcgcgg cgcaggcgct tatcgtccca
    12361 ggcaaatccc atcggttcga tgatatgcag acgaaagccg gtattagcgc aaagacggat
    12421 gatattgcca gtatttggcg gaatttctgg ttcgtaaagt acgatgttta gcatactgcc
    12481 cccttagtgc gggggcagga tagcagaaaa ttggtgagca gtgcggcctt ttcccctcac
    12541 cccggagggg cgagggagaa aaaaagagtg cggtgtcgtt tcagagtgag ggagtacata
    12601 cagcgccgaa cggtcccctc tccctgaggg agagggttag ggtgaggggg cgcaaacgac
    12661 tatgccgcat tccctttcgc catcggagcc agtgccgtag gcaactcttt acccagcccc
    12721 tgcaccagcg aatcttgcgt aatttcactg atcgatttcg cgccagtcag cgtcatcgcc
    12781 actttcatct ctttttcgat cagatttagc aggttagcta cacccgcctg gcccgctgtt
    12841 gccagcgcat acaggaaagc acgacccagc agtacggtgt cggcaccgag cgcaatcata
    12901 cgcacgacat caagcccgtt acgaattccg ctatccgcca gaatggcgat atcacctttc
    12961 accgcatctg caatagcggg caacgcacga gcggaagaga gtacaccgtc cagctggcgg
    13021 ccaccgtggt tagaaaccac aattccatca gcaccaaaac gtactgcatc gcgcgcatct
    13081 tccggatcga ggatcccttt gatcaccatc gggccatccc agaaatcgcg gatccactca
    13141 aggtctttcc atgagatgga cggatcgaag ttattcccca gccagccgat gtaatcttcc
    13201 agtccggtcg gtttgccgag ataagctgag atattaccta aatcatgtgg acgaccgttc
    13261 aggcccacat cccacgccca ttgcggatgc gtcaccgcct gcaagtagcg gcgcattgcc
    13321 gcatttgggc cgctcatacc tgaatgcgca tcacggtagc gtgcgcccgg tgtcggcata
    13381 tccacggtga aaaccagcgt cgaacaaccc gctgcttttg ctcgctccag cgcgttacgc
    13441 ataaagccgc gatcgcgcag tacataaagc tggaaccaca ttgggcgctt gatggctggc
    13501 gcgacttctt caatcgggca aacggaaacc gtcgagagag taaacggaat gccatgcgcg
    13561 tccgccgctt tggctgcctg aacttcgcca cgacgcgcat acatgccaca caaacccacc
    13621 ggtgccagtg ctaccggcat cgacaatttc tcattaaaca gcgtcgtttc caggcttaag
    13681 tcggacatgt ttttcagaat acgctggcgc agtgccactt ctgacaaatc ttccacgttg
    13741 cggcgcagcg tgtattcaga atatgcacca ccatccatat agtggaacag gaacggcggc
    13801 agaatgcgtt gcgctgcggc gcgataatcg ctggctgcgg aaataatcat gcgtttttct
    13861 ccctcgaatg ctcattatgc tcaccgggca ggcgggtaat ccgtgcgtgg cgagcctgat
    13921 cttcatcgaa tcgtttcatg gtggtgtgaa caaaactaag gtgcgccatc attgctttac
    13981 gcgccccgtc ggcatcacca gcaaaaatgg cgtcaatgac agcctgatgt tgttcggtca
    14041 gttgtgaaaa aaccggtggc accagataca tccgctgacg gctatgcttc actgaggatt
    14101 gcaggacatc gaagaaaccg cgcatggttt gcagcagcac gatgttatgt gaggcttcgg
    14161 caatcgccag atgaaaacga acgtccgctt gtgaggcgat atccgggtct tcacttagcg
    14221 ttgcttcaaa gcaaagctga atcttttctt tgtcgccagg tgtggcgcgc attgccgcat
    14281 gccatgcggt gctggcttca atggcgtagc gggcttccag aatatcgaaa ctgtaatccg
    14341 gatcatcggc catcagcgtt tttagcggct ggacgatgtt ttgctccgac catgtgtcat
    14401 gacgccagcg aataaacgtc ccgccgccgc gtcgactgag cagcacgcct tcgctcacca
    14461 gttttgctag cgcctcgcgt agtgaattac gtgatacgcc gagttgcatc gccagttggc
    14521 gctcagcggg caacttcatg cccgcttcca ggtttttttc atcaatcagc gcccgcacac
    14581 gatcggcaac ctcgtctgac aggcgtctgg gtaaaacaat cattaaggaa tcatccacgt
    14641 taagacataa gcctgaagcg tggtgatcac gcccaccata caggtgaaga tcaggctgtg
    14701 tttgacagta aagcggaaca aatcagactc tttgcccacc aggcctaccg ccgcacaggc
    14761 gatagcgata gattgcgggg agatcatctt accggtgacg ccaccggtgg tattggcggc
    14821 aaccagcaac agatcagaga cgccaatttg ttgtgctgcg gtggcttgca gcgcggcgaa
    14881 cagggcgtta gatgaggtat ccgacccggt caggaatacc cccagccagc cgaggaacgg
    14941 cgagaagaag gtgaatgcat gaccggtgtg cgccagtgcc agtgccagtg ttgatgacag
    15001 tccggaatag ttcgagataa aggcgaacgc cagcaccatg ccgatggagt agatgggtag
    15061 agccagttct ttcagcgtgc tgccgaaggt gctgatagcg tcagacggtt tcatcttcag
    15121 ccagacaatc gagagcagtg cagcaaacag aatggcggtg ccggtggcag agaaccagtc
    15181 aaacttaaac acggcggcat aggctgtagc ctcgctgacc actggtggca tacgggcaac
    15241 cagtttatcg aggtacggca ccggaatgtt gatcacccac tcatacagcg caccacccga
    15301 tgcgaacagg gctttaaacg gcgggatgct ccacagtgtt acggtagcgg tcaggaacag
    15361 gaacggtgtc caggcacgta acacctgacc cgcggtgtaa ccggtgtggg ccagcgtcat
    15421 atcaacctgt gacgccccca agtcaccgaa gcggaatacg cgaactggtt gccagcgttt
    15481 gaggaacagc gtcaggcaga gcagtgatac cagcgaagag ataatgtccg gcagctccgg
    15541 cccaatgaag ttggagctaa ggtactgagc gatggcaaac gagccgcccg caaccacgac
    15601 cgcaggccac gtctctttga taccgcgcca gccgtccata atcgccatga tccagaacag
    15661 cacgataatg gtcataaacg gtagctgacg ccccaccatc tggccaatct caaaactgtc
    15721 gatgcctgtt acttgtccgg caaccaggat tggaatgccc atcgcgccaa atgccactgg
    15781 cgcggtgtta acaatcaggc acagtccggc ggcgtacagc ggtttaaaac ccaggccgac
    15841 cagcaatgcg gcggtaattg ctaccggtgc gccaaagcct gcggctcctt caaggaaagc
    15901 gccgaaacag aaaccgacga tcagcatttg cagacgctgg tcaggggtta tcgaaagaat
    15961 agacgagcga atgatgtcaa actgcccggt tttcaccgag atcttataga cgaacactgc
    16021 tgcaataatg atccacgcga tgggccacaa accatagaag aagccataaa ccaccgaggc
    16081 cagcgcgtta gcgaccggca ttttatagaa cagcaaagcc acggcaaggg cgattgctac
    16141 cgtccatgag gcggcgacgt atcctttcag tttgagctta atcagcgcaa agaagaaaaa
    16201 caggatggga agcgatgcta tcagactgga aagccagata ttcccggcgg gatcgtagtt
    16261 ttgttgccag agattcattg caggtctcct ggagtccacg cacatcgtgt aatgagtctg
    16321 ttgctcatct ccttgtcaca cgttgtgtaa aagtggcact gccaattgtg ttgtgtaggg
    16381 ataatgacaa tgaatggtta actaaatgtt atgtattggc aatattgatg tgagttttaa
    16441 ttggggaatt gtgaaccaga ggtcagataa gcgaagaatt ggtagggcca attcttgtgg
    16501 gggggagatg tctggtaaag aaaagggcaa tcctcatcag agaattgccc ttagattgtt
    16561 gacccggagc gccttatctt gcctgatgcg cgtaacgcat caggcaattt ggcgtttgcc
    16621 cttagtcaca ctaacttaat ggatcattac cactgaatac cggcaccgag tgcggcggag
    16681 tattcaccct ggctattggt actaccttgt aatttgtaga cccaacgacc attggcgctc
    16741 accatcgata cacctaaagc aactgccgat tcaccgttgt aagtaccgcc accaatagaa
    16801 gccatgctgg cacccggtgt ataagcctgc ggcagaccgg tcattgccat tgcagaagcg
    16861 ataccaccgc tcaacttgct ttcagtttta gacagtttgt tatccatctc aaccatccgc
    16921 tgatcggtgt attgcttcgt ttcctgcacg ctttgcttca actgcgcgta gttcaccgcg
    16981 tcgttgttgt tgacgccagc ggagacgttg ctgatacgag tcgtactgcc gttgccacca
    17041 ccgagggtga tattgctata gtcgatagaa ccatcagctt tggtgtcgta gcgcacgccg
    17101 cctgcttcag aagatttcag ctgcgacacg ttaaccgcat cggtggcatt aacaccggca
    17161 gctacgttgg tgatacggcg ttggttagta gaagaaccta cagagattgt attttcttcc
    17221 tctgcaacgg aaccggtacc cagtgcgacg ctgttgtcag cggcagcaat ggaaccagaa
    17281 ccaatagcaa cgctatcttt accctgggcg ttggcatcta cgccatcggt gttggtcttg
    17341 aagtacttgg tgctaccgga ggtgacaatg tcgccaatac cgttttcgat attagtaacg
    17401 cgagtatcca gattagtgac ctgattgttc aggttagtaa tgctctgggt attctgggaa
    17461 acctgcgcat ccgttacttt caactgaccc acgttgaccg catcggtatc agccgaacct
    17521 gctgcgacgt tagtgatctg acgttgacca tccgcactac cgactgagaa ttcaccgaca
    17581 gagttctgcg gtgcgtccat gttgtaggcg gtataattgg tttgagcgcc acgagtggtc
    17641 gtagaaccat tacctatcgc aatactgttg acatgaatga cttgcgcatt gctaccaatg
    17701 gcaatgccgt taagtgcatt cgcgtccacg taggcaccat aaccgatacc aataccttta
    17761 tcgccattca cgatggtttt cgcacccatt gccagcgagt cagttcccac tgccagtgaa
    17821 tcttcttccg ttgaattagc gtggaagtat ttagtcggcg tggttgcgac cgcaccaatc
    17881 gcattctgca attgacgaac cgtaacggcg tcatgggctt cggaaccatc ggctacgttg
    17941 atgatttgac gatatttacc gtcatcaccg atagacaatg caccgagcag ttcgccatcc
    18001 gtggtgtcgt aaccaataac aacgccattt tccgttatgc tggtgtcacg ggagcctttg
    18061 gcaatcactc ggctggaaac agagctgcta cccagggcga tacccgtatc aacgtcgctg
    18121 tagctgccct gaccaatagc tacgctgcta tcgcctttgg cgacagagtt ataacctata
    18181 gctgtagcgc caacaccctg tgcgctggcg tcgttgaacg ctaagccgtc gtcgttagta
    18241 cgcacatagt taatacccgc accgttttct tggatatagg ttgcgtcggt gttaccagcg
    18301 agctgattga taatttgggt gttctgctca atcatcatat tcgtcgcatt caactgagaa
    18361 ccgttcactg catcggtact gtcctcacta atactgccat tagcgacatt agtgatgatg
    18421 ctggctttac cgtcatggct ggcattgtag gctccagcac cgccattggc agtctcatcc
    18481 cacagcaagg cgttatcatc aagcgcatcc agggcgtcac cgacgttgtt gtactcggcg
    18541 ttcacaatgg tgtaagtcgg tgcggtgatg gtgccgtcag cgttgacttc tgcgtcacca
    18601 cccagcgcat cagcgatgta cttattggtg gtatagagtt gtgagccgtt aatcgcgtcg
    18661 ctgctggcag cagagattgc accgttagcg acgttagtga ttacactggc agttttatct
    18721 tttccgtgag cggcgctaaa tgcaccattt tcaccggcgt ccgcatccca gagcagagcg
    18781 tcgtcaagag tggtatcgat agcattcagg gcatcaccga cattatcgta atcagcatta
    18841 gcaatggtgt acgtcggcgc agtgatggtg ccgtctgcat tgacttcggc accacccccc
    18901 agcgcatcaa caacataact gctcacgccg tggagttgtg agccgtttac tgcgtcagaa
    18961 ctggagtctg aaatttcacc atctgcgaca tcagtgatca cgcttgcgtc accattagta
    19021 ccgtgtttgg cactgaattt acctgcggtg gcatcccaaa gcagagcatc gccgagagac
    19081 gtgctaaatg aggagttaat cgcagccagt gcatcgccga cgttataata atttgtttca
    19141 ccgattttgt aggttggacc ggtaaacaca ccgtcagtat tcacagaagc attgccgccg
    19201 aagtaggtgg cgatattgct gcttaagtca taaagctggc taccgttaat ggcatcggaa
    19261 ctggttgcag tgactgtgcc gtccaggata ttggtgattt tgctggcggt attgttgcca
    19321 tgagctgcag tgaagacacc attgtcctta tcccatttca gcgcatcctc accaagatta
    19381 gtcaccgtct cagtcagatt agagatgttg gtggtgttag tggcgatatt ggtggtattc
    19441 gtcgccacgg catcgttggt ggttttcagc tgagagccgt taacggcatc ggtgctgcca
    19501 gtcgtcaggt cgccatcttt aacgttggtg attttgctgg tggtctcggt gccatgagct
    19561 gccgtgaata caccattgtc cttatcccat ttcagcgcat cctcaccaag attagtcacc
    19621 gtctcagtca ggttagagat gttggtggtg ttagtggcaa tattggaagt gttattggcg
    19681 atattggtgg tattcgtcgc cacagcatcg ttggtggttt tcagctgaga gccgttaaca
    19741 gcatcagtgc tgtcagccgt caggtcggca tctttaacgt tggtgatttt gctggtggta
    19801 tcctggccgt gagctgcact gaatgccttt ttagtttcgt tccagagcag ggcatcagcc
    19861 tgaaggtcac caacggaatc cgtcaggttg gtgatattgg tggtattggt ggcaatattt
    19921 gccgtattcg tggcaatgtt gctggtatta gtagcgatat tggcggtgtt ggcttcgaca
    19981 tcgtcattgg tagctttcag ttgggaaccg ttaaccgcat ccttactgga ggctgaaatc
    20041 gtgccatccg caacatcggt gattacgctg gcagttgggc tactagtacc atgagcagcg
    20101 ctgtatttgc ctttggtttg gtcccattgc agggtgtttt catcaagtac agcgagcgca
    20161 gcccctacgt tatttttgct accatttttt aaattgtagg ttggtgctgt aacagtaccg
    20221 tcatcaacat ctactgcagc ccctcctcca agcctttttg ctaccgagtc gctaatggca
    20281 taaagctgtg aaccattaat ggcatcgtaa ctgtcagact tgatcgcgcc atttttcaca
    20341 ttaacgatct tgcgttttaa tgaactattg ccgacagaga cggtattgtc taaatccgca
    20401 atggaatctg cgcccagcgc gacagagtta ataccgttgg cactgctatt actaccaagc
    20461 gcaagactct tatcaccagt tgcattactg gctgtgccta aggcaatggt gtttaagcct
    20521 ctcgactggc taccattacc cagagcgatg gcatccacgc catcagcttt gttgccctgg
    20581 cctatggcga ttgcattatc tgccagagcc tggctgccat ttcctaatgc gatagtattg
    20641 actttatcgg caaggcttcc gaaccccatt gccatgctat tagtcccttt tgccttcgca
    20701 ttattgccaa tagcagttgc attttcagcg gcttcggtct cagcacctat cgcaatagcg
    20761 ttcgctgcgc tggcaagtga ttgtctgcca aatgcgaggc tgtcagtacc gctagctaca
    20821 ctgctacgcc ccagggcaat tgcgttttct tcagatgcga cgctacttgc tcccagcgcc
    20881 attgaatacg cttttgacgc attggcggtg tcgccaagag caatactcat aatctcagta
    20941 gctttagtgg catttcccag ggcaatggcg tttgctcctt ccgctttgga agagtccccc
    21001 atagcaatag acaatttacc caatgctttt gaataacggc cgagggccat tgagtattgg
    21061 cccaatgaat acgaagaggc acccagtgct atacttctat cgccttctga tattgcacta
    21121 accccaaagg ccattgatgc accaccaatc gcatgggttt ttgacccgat ggcagagcta
    21181 tattggcctt cagctatagc gtcataaccc acagcagtac tggaaccact ggtgttcata
    21241 aaagtatttg ctttcgcccc tttgcctata gcaacccagc cgtcgccagc tgatccacta
    21301 ccgtaatcaa caccctgacc gttgtcattc ccggcattcg ccaatgcccc aaacgacgac
    21361 aacattccac ccgcaaccag cgcagaaatt aacagcttac tgcgcccaga tttcttgcca
    21421 cggctttttg ccgtttcgct ggtaacagta taattccctg tcgcagggtt ccagataact
    21481 ttaaatattt tgttcattaa tgatgctcgc tattccttgt tataagcgta ctaactgcat
    21541 ttattttttt gcttcttttc tgccgatttt tagcagaagg aagattgtcg aactgaatat
    21601 tttccatgtt gatggctaac ttcagatatc ccaggttatt gggcgataca cccttcaggg
    21661 tcaaatttca gggtcaaatt aatgtcttgc cattgacctg gtaataattt caatgtggat
    21721 ttcattgatg tgttgaaaag taattgttgc caccgggcaa actctttggc atcgacaggt
    21781 ttttcaccac cccatgtcgc attaaaacca atatttgaga taggtgtact taataagttg
    21841 accagtgaaa tatgaataac actgccattc tctgtggcgt caatggataa cactcgaccc
    21901 gctaaagcca ccgaaccatt gttcaggatg aaatacttcg tttttgctgg gttaaactcg
    21961 gtaaaaataa aagtgttttg ttgatcttta acggatttaa ttacgtgagt taaagtcttt
    22021 gtactgctta ttatgccagt ttgttgtttt tgaatagttt ccagttgact ctttagctgt
    22081 gcttgttgtt tttcaagtag atcaatttta ttgttatgtt cttcttgttg tttcaataaa
    22141 ctactttctg gcttgccttc ttcaatgtag tcacatcccg ttaatgcgca gagaactgaa
    22201 acagtgacag cactgaaaaa aaatcttctt caagttcatg taaaaatcct tatttcctcc
    22261 gaccagcgat caatgccggt agagttgtcc attgtattat tggtgatcta tgttgcttta
    22321 cattaatgac ag
//
LOCUS       DAZUFB010000076        22168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZUFB010000076 DAZUFB010000000
VERSION     DAZUFB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.076"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..862
                     /locus_tag="ACHY3M_003284"
     CDS             2..862
                     /locus_tag="ACHY3M_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFS4318139.1"
                     /translation="MLMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKL
                     ESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVR
                     IQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNP
                     SESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFS
                     DKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGS
                     SGGGGASGRW"
     gene            complement(922..2220)
                     /gene="eno"
                     /locus_tag="ACHY3M_003285"
     CDS             complement(922..2220)
                     /gene="eno"
                     /locus_tag="ACHY3M_003285"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4318140.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2308..3945)
                     /gene="pyrG"
                     /locus_tag="ACHY3M_003286"
     CDS             complement(2308..3945)
                     /gene="pyrG"
                     /locus_tag="ACHY3M_003286"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFS4318141.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4173..4964)
                     /gene="mazG"
                     /locus_tag="ACHY3M_003287"
     CDS             complement(4173..4964)
                     /gene="mazG"
                     /locus_tag="ACHY3M_003287"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFS4318142.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5035..5370)
                     /gene="mazF"
                     /locus_tag="ACHY3M_003288"
     CDS             complement(5035..5370)
                     /gene="mazF"
                     /locus_tag="ACHY3M_003288"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFS4318143.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5370..5618)
                     /gene="mazE"
                     /locus_tag="ACHY3M_003289"
     CDS             complement(5370..5618)
                     /gene="mazE"
                     /locus_tag="ACHY3M_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFS4318144.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5696..7930)
                     /gene="relA"
                     /locus_tag="ACHY3M_003290"
     CDS             complement(5696..7930)
                     /gene="relA"
                     /locus_tag="ACHY3M_003290"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFS4318145.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7978..9278)
                     /gene="rlmD"
                     /locus_tag="ACHY3M_003291"
                     /pseudo
     CDS             complement(7978..9278)
                     /gene="rlmD"
                     /locus_tag="ACHY3M_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            9335..12091
                     /gene="barA"
                     /locus_tag="ACHY3M_003292"
     CDS             9335..12091
                     /gene="barA"
                     /locus_tag="ACHY3M_003292"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFS4318146.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVMNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12323..13663)
                     /gene="gudD"
                     /locus_tag="ACHY3M_003293"
     CDS             complement(12323..13663)
                     /gene="gudD"
                     /locus_tag="ACHY3M_003293"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFS4318147.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13684..15024)
                     /locus_tag="ACHY3M_003294"
     CDS             complement(13684..15024)
                     /locus_tag="ACHY3M_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFS4318148.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQVADFDTFGKSA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15026..16378)
                     /gene="gudP"
                     /locus_tag="ACHY3M_003295"
     CDS             complement(15026..16378)
                     /gene="gudP"
                     /locus_tag="ACHY3M_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFS4318149.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16813..17262)
                     /locus_tag="ACHY3M_003296"
     CDS             complement(16813..17262)
                     /locus_tag="ACHY3M_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4318150.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17280..18062)
                     /gene="truC"
                     /locus_tag="ACHY3M_003297"
     CDS             complement(17280..18062)
                     /gene="truC"
                     /locus_tag="ACHY3M_003297"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFS4318151.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18062..18391)
                     /locus_tag="ACHY3M_003298"
     CDS             complement(18062..18391)
                     /locus_tag="ACHY3M_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFS4318152.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19013..19558)
                     /gene="syd"
                     /locus_tag="ACHY3M_003299"
     CDS             complement(19013..19558)
                     /gene="syd"
                     /locus_tag="ACHY3M_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFS4318153.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQSTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19626..20474
                     /gene="queF"
                     /locus_tag="ACHY3M_003300"
     CDS             19626..20474
                     /gene="queF"
                     /locus_tag="ACHY3M_003300"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFS4318154.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20586..21950
                     /gene="ppnN"
                     /locus_tag="ACHY3M_003301"
     CDS             20586..21950
                     /gene="ppnN"
                     /locus_tag="ACHY3M_003301"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFS4318155.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5449 a         5910 c         5248 g         5561 t
ORIGIN      
        1 catactaatg ttcactttcg tgtgttcctt tgttgcagcc caaccaacta ttgtcccaca
       61 attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa agaaagaact
      121 gacgcacaag ttagaatcta ttttcaataa tacccaagtg caaattgctg tattaattgt
      181 tcccacaacc aaagacgaga caattgaaca atatgctaca agagtttttg acaattggcg
      241 tttaggagat gccaaacgta atgatgggat actgatcatt gttgcctggt cagatcgtac
      301 tgtccgcatc caggtaggtt atgggctgga agaaaaggta accgatgctc tggctggaga
      361 tatcatccgt agcaacatga tacccgcatt taaacaacaa aaatttgctc agggattaga
      421 gctagctata aacgctttga ataaccaact cacttcacaa caccaatatc cgactaatcc
      481 ttcagaaagt gaatcagcgt cttccagtga tcattattac tttgctattt tttgggtatt
      541 tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt gtcgcgcatg
      601 taaaagtgga gtctatattt cagcaatcta tcttttagat ttattcctgt tctccgataa
      661 aattttttcc attgctgtat tttccttctt ttttactttc accatattta tggtctttac
      721 ctgtttatgt gtacttcaga aaagagcatc aggtaaaagt tatcattcag acaacagcgg
      781 ttccggtgga ggctcaagtg gcggcggatt tagcggcggt ggcggttctt ctggaggtgg
      841 cggcgcatcc ggccgctggt aactcaacca taaaaaatgc cagcccggag gctggcattt
      901 ttaaatcaga taaagtcagt cttatgcctg gcctttgatc tctttacgac cgttgtacgg
      961 tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc
     1021 agaacggctc atagaaccag ttttgatctg gcctgcagca gtaccaacag ccaggtcagc
     1081 gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt agccagcatc
     1141 tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat
     1201 caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac
     1261 gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa
     1321 accgtcccag tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt
     1381 cagttcttcc aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc
     1441 cagaacgtat ttaccgtctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat
     1501 gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc
     1561 agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaactgcag tgttcatgcc
     1621 tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc
     1681 tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc
     1741 agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt atttgcccgg
     1801 agtaccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc
     1861 tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc
     1921 ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc
     1981 aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc
     2041 caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc
     2101 acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac
     2161 agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat
     2221 taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaggg
     2281 tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc
     2341 ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg
     2401 atggaactgg caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg
     2461 atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat
     2521 gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg
     2581 gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc
     2641 gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat
     2701 cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg cgttctccat
     2761 gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag
     2821 ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg
     2881 atagccgaaa ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg
     2941 cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc
     3001 accgtgtttc agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc
     3061 gaccataccg atggtgactt cacttaccgg gttcgcttct tcgaagataa cctgttccca
     3121 ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag
     3181 cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat
     3241 aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac
     3301 agcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt
     3361 tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag
     3421 cgtcaggtgc ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatcgc
     3481 ttcgaggaac ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac
     3541 gtcatgacct tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat
     3601 aacctgcacg gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta
     3661 gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta
     3721 gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg
     3781 gattgggctc atagtacctg gatcgacgtt gatgtacgga tccagtttca tgatggtcac
     3841 attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag
     3901 agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag
     3961 ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg aacatgacct
     4021 gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag
     4081 aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa
     4141 tgacacaaat ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc
     4201 tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg
     4261 gcaacaatac gctccacttc gcgaaaacga cgctcaaatt tttcgttcgc tttttgcaat
     4321 gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa cagcaggtcc
     4381 cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcttc gtacatcacc
     4441 tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca atcgaagcca
     4501 acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa actacgagga
     4561 atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc
     4621 caacgggcaa gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg
     4681 cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct
     4741 tcctgagcca tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga
     4801 agatcgtcaa aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg
     4861 taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg
     4921 cgcaggcgct gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa
     4981 ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa gtgagttagt aacactaccc
     5041 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt
     5101 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc
     5161 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtata
     5221 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc
     5281 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc
     5341 gcccatatcg ggtacgtatc ggcttaccat taccagactt ccttatcttt cggctctccc
     5401 cagtcgatat tctcgtggag gttttccggc gtgatgtcgt tgaccagttc agcaagcgta
     5461 aatacgggct ctttacgcac tggctcaata attaatttgc catccaccag gtcaatcttc
     5521 acttcatcat caatattcag attgagcgcc tgcattaacg tagccgggat ccgcaccgcc
     5581 ggtgaatttc cccaacgctt tacgctactg tggatcataa ccctttcctc aaaccgctat
     5641 catatgtaga tacagtatat atcaatctac attgtagata cgagcaaatt tcggcctaac
     5701 tcccgtgcaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc
     5761 gccccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt
     5821 tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg
     5881 tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc
     5941 cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca
     6001 gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc tgggtaatga
     6061 agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt
     6121 tgccaacacc ttcgaccacc acgcgaccgt tatctttact gcggttttgc ggcgtgtagc
     6181 ttttttgctg aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa
     6241 attgcgattg caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg
     6301 ccagcaactc gtcgacatca ttaaagttgt aacgcggcag cagatgtttt tctgcttctt
     6361 tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt
     6421 ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg
     6481 tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga
     6541 taatttcaat ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa
     6601 tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg
     6661 gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt
     6721 cgtcaaagac ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct
     6781 gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc
     6841 cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactct gcatcttcat
     6901 gcatctgttt ggtgcggatt tggatctcaa cggttttgcc acctggcccc agaaccacgg
     6961 tatgaataga ctgataaccg tttggtttcg ggttagcgac ataatcgtca aactcatccg
     7021 gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct
     7081 cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct
     7141 gcattttgcg ccagatactg tagatgtgtt tcggacgacc atacacttca gctttaacgc
     7201 cttcagcttt catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt
     7261 cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac
     7321 ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg
     7381 gcgcgtagat attggtgcac tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt
     7441 ttacttcgcg cagatgagca atacgctccg ccagtttgat gaccacgcag cgaaaatcat
     7501 cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag
     7561 tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa
     7621 cgaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca
     7681 gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct
     7741 ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt
     7801 gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca
     7861 gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta
     7921 ccgcaaccat tgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta
     7981 tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag
     8041 catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag
     8101 cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta taatttgctg
     8161 cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt
     8221 cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg
     8281 taaaccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac
     8341 accgaccaca ctggctgctt gtttcgccaa tggcagtgta aagttgccca taccgcagaa
     8401 cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat
     8461 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8521 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8581 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     8641 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     8701 tcaacatgac caagatggcg cataacttgt aagctgccca ggcatgccct gactttgggc
     8761 agcaatgctt caagttgggg cactaaaatg gggcattgtt taacgtcgac aatgtcactg
     8821 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     8881 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     8941 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatctac gctggcgtgt
     9001 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggtgtttc gcgttccggg
     9061 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9121 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9181 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9241 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9301 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9361 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9421 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ccagcattat
     9481 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9541 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9601 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
     9661 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
     9721 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
     9781 tgccaaaaat agtcaaaata tgttgggata tattgcgctg gagctggatc ttaaatcggt
     9841 tcgcttgcag caatataaag agatctttat ttccagcgtg atgatgctgt tttgtatcgg
     9901 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
     9961 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10021 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10081 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10141 ggagcagatg gaaattcaga acgttgagtt agatctggcg aaaaagcgcg cccaggaagc
    10201 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10261 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10321 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10381 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10441 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10501 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10561 acagcaaatc atcactaacc tggtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10621 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    10681 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    10741 acaggctgat gccagtattt cccgccgtca tggtggcacc ggtctggggc tggtgattac
    10801 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    10861 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    10921 atccacccag tgcctcgccg gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    10981 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11041 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11101 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11161 gatgctggca cttccttgcc atgcacaagt caatgcagaa aaactcaagc aagatggtat
    11221 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11281 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagcaagc tggcaatgac
    11341 agtcatggca gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11401 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcggttgaac gggcgaaaca
    11461 gatgccgttc gatttgatct taatggatat tcaaatgcct gacatggatg gcattcgggc
    11521 ctgcgagctc atccaccagc tcccccatca gcaacaaacg ccggttatcg cggtaacggc
    11581 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    11641 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    11701 tatttcctct cgcgtcgtga cgcccgaagt caacgaaatt gtgatgaacc cgaatgcgac
    11761 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    11821 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    11881 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    11941 tagcggtgtg ccgcgtatga agaatctctg ccaactgatc gaacaacagc tacgtagcgg
    12001 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12061 agcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12121 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12181 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12241 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaagccg gctccagata
    12301 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12361 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12421 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12481 tttccggcac ctgaaccaga ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12541 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12601 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    12661 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcatc gtccagaaat
    12721 gcggatccgc cagcgggata tcaacggatt gcagggagag cgtatggccc atctgccgcc
    12781 agtcggtggc gatcatattg gttgcggtcg gcaggccagt tgcgcgacgg aactcagcca
    12841 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    12901 accctttcag gtatttacca attttaatcg cttcgttcag cgaccaggca ccgttaggat
    12961 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcctctt
    13021 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13081 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13141 accagtcgca tgagtcatcc ggctggcttt gatacggcag cggtgtggct ttgcgatcac
    13201 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13261 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13321 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccaccagcat
    13381 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13441 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13501 tttcccctac gccagtgtga ccagaattat ctttgataat cacaatatta cgcgtaaaga
    13561 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13621 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    13681 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    13741 ctgcatcgga cctgcgtcgt tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc
    13801 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    13861 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    13921 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaacat
    13981 cgccagagag atatcgaaat ggttattaga atggcagccc caggtcagcc cccagtcgtc
    14041 gcaaagctgc gccacacgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14101 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14161 gttagtcgcg acgggcaagc cggtcgcccg ccgaaactcc gccatcactt cacgaccgga
    14221 gaaaccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14281 gcatagagaa atggcttcat caagtagcca ggcaccgttg ggatcaacgg taatccgcgc
    14341 atcagggaag cgtttcttca atgcccgaac agtgtcgatt tcttgctcgc caggtaacac
    14401 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14461 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14521 cggcgtattt tccagataag gaagatcggt tttggtccga tcaccgatat aaaacagata
    14581 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    14641 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    14701 gcgcaattca aaagtccagg cacttttgcc gaaggtatca aaatcggcta cctgattacc
    14761 tttatgcacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    14821 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    14881 atgcccggcg ttatcggtga gtaccacaat attgcgggtg aaatatgcgt tatgtgcccc
    14941 accaatatta agcaacatgc tatcgtgccc ggcgaccgga atgactttca tatcagtaat
    15001 aacaggactg gattgtgtcg tcatcttatt gccccgcgac aggtttcagc tcgatacgct
    15061 tgatatcgcc caccagcacc aggtagctca gtaccgcgat taaggcatga acaccaacat
    15121 aaatcagcgc cccattaaag gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15181 tgacgatgcc agaaatgtta ccgaacatgt tgaacaggcc accggaaaga ccgctgatct
    15241 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15301 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15361 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15421 ccgtgcggcg catcagccag tcggaaataa tcccacccag cacaccgccg ataaaaccgc
    15481 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15541 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15601 cgatataaac gccgatcatc atccgtgacc cgagcagttg tttgatctgc ccccacttca
    15661 cgctgaacgg cactttaact ttggtgtttt gctgatccat attgatcagc gcgccacccg
    15721 cggcgatgta ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    15781 ctttcaacca gataaagctg atgacaatcc ccagaccgcc cataaagaag aagacgtgtg
    15841 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    15901 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    15961 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16021 gcgtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16081 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16141 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16201 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccagcaatag
    16261 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16321 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16381 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16441 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16501 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16561 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16621 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    16681 gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag
    16741 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    16801 tcgctgaaca tatcaggaca acagcgtgcc ccactgttcg acccacggat ttgattccgt
    16861 ttccggttcc gggttctcac tggcgtcaat caacagtatt tcgccaaccc gctgagcgct
    16921 ctgttcctgc aacaaggcat cgaactgttt gccgccattg cagaaattta cataactgct
    16981 gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccagac tatctttgat
    17041 tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac
    17101 caccagaaca tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac
    17161 cgttgctttg tggccctgtg cggtcagaat tgcttctgct tcttccgcca ctaacagcga
    17221 atttccgtac atggtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat
    17281 taggaactta tctctccatc ctgacccgat ggcgcactga actcaaccct ttcattttca
    17341 ggaagcagac cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc
    17401 gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca atgacaactg actggcatgt
    17461 agcattaacc gctggaggcc aaaatgctca gcaccgctgc gattctggcg taaatcgcca
    17521 tgtttgctat cgccaataat cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt
    17581 ttgcgtccgg ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg
    17641 gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcgag
    17701 cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa
    17761 tccagaactg cttcttccat caaccagccg cgcacaatcg catggtaacg tttctggatt
    17821 tggtgctgtt caaactgttg tgccagcagc cgtccggcct cgctggataa tcccatcaac
    17881 aacacaccag aagtgggtcg gtccagacga tgagcggtaa aaacgtgctg gccaatctga
    17941 tcacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct gcggtgaacc
    18001 agccagccgg agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc
    18061 attagctcgc atcatccgca aaaagagcat ccagtttttc cagctcagcc agaataagcg
    18121 cgcgttgcgg atggtccgtc gccagcgcca tttcataata gggtgcaacg gcaaaagcgc
    18181 ccggtaacgg ctgtttgtta tctaacaaat cgtgcattcg cgggatcagc acccactgca
    18241 accactccag tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg
    18301 gctggggttc atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca
    18361 actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc
    18421 gaaggatagc aggaataaaa aaagggagca ctgtattcac agcgctcccg gttcgtttcg
    18481 cagcattcca gctacttttg ttgctccctg ctcatccttg acaacttttc ctctggcctt
    18541 gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt
    18601 catcctgaag tgtccctggc cttcctgacc caccgaatca tcctgaccgg ttctcattct
    18661 ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc
    18721 tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt catcatcctg atgttcactt
    18781 cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc
    18841 gcttacagac aatgccttaa ggaaaatttc atatgaaagt taagaattaa caatcaacat
    18901 tttgatttta aatgaaaata acgattctta tctttttaag tattcgctta tttcctggcg
    18961 atctcctaca gagtctgtaa gagatctctc acaaccagat ggggtatttg gattacagaa
    19021 gaggcttaag ttgattaagg aattccgcaa gatttgaggc taaatgcgtc cgtttacgtg
    19081 ttcccagcgt ctctttgcac acttcaccgg agagattaca caccgagatg acctctaact
    19141 cttcttccag cgtcgcgata aatagcgtgg gcggcagttt tagccttttc tgagtcacca
    19201 gatgaccaat cagattttcc tgcacccgac ggaagtcgtc ttcactccag gtttgcagca
    19261 atgtcagttt gatatcgcca aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa
    19321 aggtatgaat tgtggattgt atcacaatat caaaagcgcg ttcaaccgcg tttacatttt
    19381 gttccccggt aaacggctgg ggttgccagt atacggcatc ttcggtagtt gaaataatgc
    19441 acggtgaagg aacaccgtac agttcctcac ttaacggcca gcttttatgc tcttcatgcc
    19501 acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta
    19561 atttctctct tcacttaaac cagatacact tgtcgtttag tttatctggt ttatgacggt
    19621 gaaacatgtc ttcttatgca aaccatcagg cacttgcggg cctgactctt ggaaaatcaa
    19681 ccgattaccg ggatacctat gacgccaacc tactgcaagg cgttccacgc agcctgaatc
    19741 gcgacccgct gggtctgaaa gcggataacc tgccttttca cggtacggat atctggacgc
    19801 tgtatgaact ttcctggctg aatgcgaaag gtttgccgca ggttgccgtc ggtcacgttg
    19861 aacttgatta caccagcgca aatctgattg agtcgaagag ttttaagctc tatctcaaca
    19921 gttttaacca gacgcgtttt aataactggg atgaggtgcg ccagacgctg gagcgcgact
    19981 taagcacttg cgcccagggt aaggttagcg tggcgttata tcgtcttgat gaactggaag
    20041 gccagccgat aggtcatttc aatggcacct gcattgatga ccaggatatc actatcgata
    20101 actatgaatt cactactgac tatctggaga atgccaccag cggtgaaaaa gtagtggaag
    20161 agacgcttgt cagccacctg ctgaaatcaa actgcctgat cacccatcaa ccagattggg
    20221 gttcgatcca aattcagtat cgtggacgcc aaattgacag agaaaaactg ctgcgttacc
    20281 tggtctcatt ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc atctttaatg
    20341 acctgttacg cttctgccag ccagagaaat taagcgttta cgcacgttat acccgtcgtg
    20401 gcggtctgga cattaacccg tggcgcagta atagcgattt tgtcccatcg accacaagac
    20461 tggttcggca ataaattttt tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa
    20521 aggtcatcag gcagggctat tgtaatcaaa gggaatgacg atattcgtcc tataaggagt
    20581 ttttcttgat tacacatatt agcccgcttg gctccatgga tatgttgtcg cagctggaag
    20641 tggatatgct taaacgcacc gccagcagcg acctctatca actgtttcgc aactgttcac
    20701 ttgccgtact gaactccggt agtttgaccg ataacagcaa agaattgctg tctcgttttg
    20761 aaaatttcga tattaacgtc ctgcgccgtg aacgcggcgt aaagctggaa ctgattaatc
    20821 ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg
    20881 cggttctgcg agacattctc ttcgtttacg ggcaaatcca taataccgtt cgttttccca
    20941 acctgaatct cgacaactcc gtccacatca ctaacctggt cttttccatc ttgcgtaacg
    21001 ctcgcgcgct gcatgtgggt gaagcgccaa atatggtggt ctgctggggc ggtcactcaa
    21061 ttaacgaaaa cgagtatttg tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc
    21121 tgaatatctg caccggctgt ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg
    21181 tcggacacgc gcagcagcgt tacaaagaca gtcgttttat tggtatgacc gagccgtcga
    21241 ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg ccagatatcg
    21301 aaaaacgtct ggaagcgttt gtccgtatcg ctcacggtat cattatcttc cctggcggtg
    21361 tgggtacggc agaagagttg ctgtatttgc tgggaatttt aatgaacccg gccaacaaag
    21421 atcaggtttt accattgatc ctcaccggcc cgaaagagag cgccgactac ttccgcgtac
    21481 tggacgagtt tgtcgtacat acgctgggcg aaaacgcgcg ccgccattac cgcatcatca
    21541 ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa
    21601 atcgccgcga cactggtgac gcttacagct ttaactggtc aatgcgcatt gcaccagatt
    21661 tgcaaatgcc gtttgagccg tctcacgaga atatggctaa tctgaagctt tacccggatc
    21721 aacctgttga agtgctggct gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta
    21781 acgtaaaaga agtcggtatt cgcgccattg aagagtttgg tccttacaaa atcaacggcg
    21841 ataaagagat tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc cagcatcgta
    21901 tgaagttgcc aggctcagcc tacatccctt gctacgaaat ctgcacgtaa tctcccctcc
    21961 cccggtgacg tttgttgccg ggttttctcg tttttggtca cttactcatc aactcatttc
    22021 atttgttata tgaatgtttc ttaccaccct cacgcgacaa atatcatcac agttaatatg
    22081 tcatacaatt tatattgcaa cgcaaacgtt tacctatttt cataaaccgt attttatcgc
    22141 tatgaaaaag aaattatcgc catgatta
//
LOCUS       DAZUFB010000077        21976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZUFB010000077 DAZUFB010000000
VERSION     DAZUFB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.077"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..401
                     /locus_tag="ACHY3M_003302"
     CDS             129..401
                     /locus_tag="ACHY3M_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFS4318156.1"
                     /translation="MALPRIPQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(402..1274)
                     /gene="rluF"
                     /locus_tag="ACHY3M_003303"
     CDS             complement(402..1274)
                     /gene="rluF"
                     /locus_tag="ACHY3M_003303"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFS4318157.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1623..2571
                     /locus_tag="ACHY3M_003304"
                     /pseudo
     CDS             1623..2571
                     /locus_tag="ACHY3M_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            2641..3447
                     /locus_tag="ACHY3M_003305"
     CDS             2641..3447
                     /locus_tag="ACHY3M_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4318158.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3457..3864
                     /locus_tag="ACHY3M_003306"
     CDS             3457..3864
                     /locus_tag="ACHY3M_003306"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFS4318159.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3864..4358
                     /locus_tag="ACHY3M_003307"
     CDS             3864..4358
                     /locus_tag="ACHY3M_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4318160.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4424..5221
                     /locus_tag="ACHY3M_003308"
     CDS             4424..5221
                     /locus_tag="ACHY3M_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4318161.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEAWFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5232..6056
                     /locus_tag="ACHY3M_003309"
     CDS             5232..6056
                     /locus_tag="ACHY3M_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4318162.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCLGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6108..7337
                     /gene="sorE"
                     /locus_tag="ACHY3M_003310"
     CDS             6108..7337
                     /gene="sorE"
                     /locus_tag="ACHY3M_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFS4318163.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7720..8409
                     /gene="pepE"
                     /locus_tag="ACHY3M_003311"
     CDS             7720..8409
                     /gene="pepE"
                     /locus_tag="ACHY3M_003311"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFS4318164.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8500..10131)
                     /locus_tag="ACHY3M_003312"
     CDS             complement(8500..10131)
                     /locus_tag="ACHY3M_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFS4318165.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10351..14034)
                     /gene="metH"
                     /locus_tag="ACHY3M_003313"
     CDS             complement(10351..14034)
                     /gene="metH"
                     /locus_tag="ACHY3M_003313"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4318166.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14234..15058
                     /gene="iclR"
                     /locus_tag="ACHY3M_003314"
     CDS             14234..15058
                     /gene="iclR"
                     /locus_tag="ACHY3M_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFS4318167.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15375..16949
                     /locus_tag="ACHY3M_003315"
     CDS             15375..16949
                     /locus_tag="ACHY3M_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFS4318168.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNA
                     LPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(16918..18654)
                     /gene="aceK"
                     /locus_tag="ACHY3M_003316"
     CDS             complement(16918..18654)
                     /gene="aceK"
                     /locus_tag="ACHY3M_003316"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFS4318169.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTNDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(18935..20239)
                     /gene="aceA"
                     /locus_tag="ACHY3M_003317"
     CDS             complement(18935..20239)
                     /gene="aceA"
                     /locus_tag="ACHY3M_003317"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFS4318170.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20269..21870)
                     /gene="aceB"
                     /locus_tag="ACHY3M_003318"
     CDS             complement(20269..21870)
                     /gene="aceB"
                     /locus_tag="ACHY3M_003318"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFS4318171.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         5363 a         5705 c         5589 g         5319 t
ORIGIN      
        1 tgtatctgta tttatcgtca attatcaaga aagataaata tttaagaaga gaacaaagga
       61 atattatcag ttcagctcgc aaacgaattc ccgtacactg aactctctgg ctcactgagg
      121 gcaccatcat ggcactcccc cgtattcccc aaaaagagat gactgaacgc gagcagcgtg
      181 aacttaaaac actgctggat cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg
      241 aaaccaacag cattaagaaa gagtacatcg ataaattaat ggtcgagcgt gaggctgaag
      301 cgaaaaaagc ccgccagttg aagaaaaagc aggcttataa accggatcct gaagcgtcat
      361 ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg attagcgtcc cttcttttta
      421 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
      481 ttttccatct taacgactgg acgtttgatg cccgctgttt tcggtttagc cttcgcttta
      541 ggttttacct cggaagagga attttcaatg agtttaaaca ggtcgattaa ctcatcgtca
      601 gttaaatcgc gccactcacc cagcggaatg ccgcttaaac tgacgttcat aatgcgcgta
      661 cgttccagct ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc
      721 agcccctgca ccagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt
      781 ttcgtcaccg tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta
      841 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
      901 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
      961 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
     1021 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
     1081 tcggcttccc gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc
     1141 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
     1201 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
     1261 gagtcgggca gcataaattc tcctgtaaaa cggcggaata taccgtacct cttagcgggg
     1321 aaaaacaggg ttatgcggca acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca
     1381 aaagtgtctc cggaagtacg atgcaaattt gcctggcaaa ataaggataa tcaacatatt
     1441 acgaatcgct gcacacctgt gctgatgact actacgcaga gaaacaaatg tgcaagagaa
     1501 agcgctattt ctccccatgc aaagagtgaa aaatcgcttt gaaaggccct catgatgggg
     1561 ttatatcaga tcaagcggtg agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg
     1621 caatggaaaa cagtgacgat atccgtttga ttgtgaagat tgcccaactc tattacgaac
     1681 aggatatgac gcaggcgcaa atcgcgcgcg aactgcgtat ttaccgcacc accatcagcc
     1741 gcttgcttaa acgaggccgc gatcagggaa ttgtcaccat cgccatcaac tatgactaca
     1801 acgaaaatct ctggctggag cagcaactga agcaaaagtt tggcctgaaa gacgttgtgg
     1861 tggtgtcggg aaatgatgag gatgaagaga ctcaactggc gatgatgggg ttacacggcg
     1921 cgcaactgct ggatcgcttg ctggagcctg gcgatattgt cggtttttcc tggggtcgcg
     1981 cggtgagcgc actggttgaa aacttgccgc aggcggggca atcgcggcag ttaatctgcg
     2041 taccaattat tggcggaccg tccggtaaac tcgaaagccg ctatcatgta aacacattaa
     2101 cctacagcgc ggcagcgaag ctgaaagggg aatcgcatct cgcgtatttt ccggctctgc
     2161 tggataaccc attaattcgt aatgggatca tgcagtctca gcactttaaa accatctctg
     2221 cctactggga taatctggat gtcgccctgg tgggaattgg ctcaccggcc attcgcgacg
     2281 gcgctaactg gcatgcgttt tatggtggtg aagagagtga cgacctgaat gcccgccagg
     2341 ttgctggcga tatttgctcg cgcttttttg atattcacgg cgcaatggtt gaaacgaata
     2401 tgagcgaaaa aacgctctct atcgaaatga ataaattaaa gcaggcgcga tattccattg
     2461 gcattgccat gagtgaagaa aaatacagcg gaattattgg ttgcactgcg tggaaaatat
     2521 attaattgtc tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc
     2581 cgatgtttac cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt
     2641 atgcaaacgt ggttaaattt gcaggataaa atcattatcg tcaccggcgg cgcatccggt
     2701 attggtctgg cgattgtaga ggaattatta gcacaaggcg caaacgtaca gatggtagat
     2761 attcacggtg gcgatggtca atatgaaggt cataaaggct atcagttttg gccgaccgat
     2821 atttccagcg ccaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc
     2881 atcgacggtc tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa
     2941 gcgcctgccg ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac
     3001 cagaaaggcg tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat
     3061 ggcgtgattg tgaatgtttc ctcagaaagt ggactggaag gctcagaagg ccaaagctgt
     3121 tacgccgcta ccaaagccgc gcttaatagc ttcacgcgct cctggtcgaa agagctgggt
     3181 aaacacggta tccgtgtggt cggtatcgcg cccgggattc tggaaaaaac cgggctacgc
     3241 acgccggaat atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga gcagctgcgt
     3301 gaaggctata ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtcgct
     3361 gattttgttt gttatctgtt gtctgaacgc gccagctaca tcaccggagt aaccactaac
     3421 attgcgggcg gcaaaacgcg cggctaagga ggctttatgg tcaacgccat tttttgtgcc
     3481 cacggcaaat tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacgccaat
     3541 gttgaagccg ttgaatttgt gcccggcgag aacgccggag acatcgtagc aaagctggaa
     3601 aagttagtaa gcattcacaa tcaagatgag tggctgattg ccgtggattt acagtgcggc
     3661 agcccgtgga atgcggcagc gatgcttgcg atgcgcaatc ctcgtctgcg ggtaatcagc
     3721 ggcctttctc tgccgctggc gctcgaactg gttgataacc aggacagcat gaatgttgat
     3781 gaactgtgtg aacacctgac gcaaatcgca aagcaaacct gcgttgtctg gaaacaactg
     3841 gcaacggcag aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgcctga
     3901 tccacggtca ggtcactacc gtctggtcga aagtggctaa cgcccagcgc attattatct
     3961 gcaatgacga agtttataac gatgaagttc gccggacatt attgcgtcag gctgcgccac
     4021 cgggaatgaa ggttaacgtg gtcaatattg aaaaagccgt cgctgtttat cacaacccgc
     4081 aatatcagga cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg
     4141 ttcgccaggg ggtaaaaata gacaccctga atattggcgg catggcctgg cggcccggta
     4201 aaaaacagtt aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt
     4261 taaataatct tggcgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata
     4321 ttatcgacaa aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat
     4381 gctttaacag gcagggatga cgacacttaa aaggtgaaaa atcatggaaa taagtaccct
     4441 acaaatcatt gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga
     4501 atttcagact catcgcccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt
     4561 aaaaaccgga ataatactcg gtggtacgct ggaattgata gctctcggct ggatgaacgt
     4621 cggcgcggcg caatctccgg attctgcact cgccagcata atctccgcca ttctggttat
     4681 cgttggtcag caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg
     4741 ccaggtgctg actgtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa
     4801 agcagcggaa gaagcatggt ttcgcactct cgatattctg catgtctccg cgcttggcgt
     4861 gcaggcgctg cgcgttgcta ttccggcact gattgtctca ctgttcgtca gcgccgatat
     4921 ggtgagcaat atgctgagcg cgataccgga attcgtgacg cgcggtctgc aaattgctgg
     4981 cggttttatc gtggtggtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt
     5041 gatgcctttc tttttcctcg gtttcctcgc aggtggctac ctcgatctca gtctgctggc
     5101 gttcggtggc gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg
     5161 taaagctgaa cctcaacccc aggccaccac ctccaccgcc cttgaccaac ttgacgatta
     5221 acggagccat catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc
     5281 gctccaacct gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggcttttgct
     5341 acgacatgat ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc
     5401 tcaggcgaca cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg
     5461 tcactgccgc catggaagag gcgcgggcca acggcgcgga aattgatgac ggtaccatta
     5521 acggcatcaa agtcggtctg atgggaccat tggcaggggt tggcgatcca ctggtttggg
     5581 gaacgctgcg cccgattacc gctgcgctcg gcgcatctct ggcactttct ggcaacatcc
     5641 tcggcccgct tttgtttttc tttattttca atgcggtgcg tctggcgatg aagtggtatg
     5701 gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc
     5761 tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtca
     5821 ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc gccgatggct
     5881 ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccttggt ttgctggcgc
     5941 tcggtctgac gctgcttatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga
     6001 ttttcgccct gtttggctta gggattatcg gtaatgcgct gggcttcctg tcctgattct
     6061 tcgccccggc acgactgccg gggccatcgc tcaacatgag gtggtttatg aaaacaacag
     6121 ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt cctgaaatgc
     6181 aggaggatga aatcctcgcg acggtggtca ctgacagcct gtgcctctct tcctggaaag
     6241 aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aaccccatca
     6301 tcatcggcca cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat
     6361 tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac cgcccggact
     6421 gccccggcta ctccttcccg tgggtgggcg gcgaagccac gcatgtggtt attcccaacg
     6481 aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt gaaggctcac
     6541 tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag
     6601 gtagttataa ccacacgatg gggattcgcc cgcaagggcg catgctgatc ctcggcggca
     6661 ccggaccaat gggactgttg gcgattgatt atgcgctaca tggacccgtt aacccgtcgc
     6721 tgctcgtcat taccgatacc gacaacgata aattgagtta tgcgcgcaag cactatccat
     6781 cagaaccgca aacactgatt cattatctca atgccgccga tgcagcattt gatacgctaa
     6841 tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac
     6901 tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac
     6961 cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc
     7021 actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc aaactgattg
     7081 aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg
     7141 gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg tataccggga
     7201 aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctgg
     7261 cgcgtcatca ggggatctgg tccggtgagg cggagcaata tctgctcact catgcagagg
     7321 caatttccca tgattaatca cgctacttaa ctctgcatgt cgctggctgg tcgccgcggc
     7381 aacttcgcga cctgatgtgc ggctgattgc tgaagcggcc caatgtgcac gagaaggata
     7441 aaaaaacatc agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc
     7501 cggatgcgga ataaagcctt attcggccta cataatctcg aaaattcaat atattgcagt
     7561 tatctggtag gcctggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca
     7621 gcgcccggtg caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg
     7681 tagcatgcct cccgtgattt cttactaact ggagaagtaa tggaactgct tttattgagt
     7741 aactcgacgc tgccgggtaa agcctggctg gaacatgcgc tgccgctgat tgctgaacag
     7801 ttgcagggtc gccgttcagc ggtatttatt cctttcgctg gcgtaacgca gacctgggat
     7861 gattacacag cgaaaacggc tgcggttctc gctccgctgg gtgtatctgt caccggtatt
     7921 catagcgttg tcgatcccgt tgccgcgatt gaaaatgctg agatcgtgat tgtcgggggc
     7981 gggaatactt tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc accaattact
     8041 gacgtggtta aacgtggtgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc
     8101 ccaaccattc gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta
     8161 aatctgttcc cgctgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa
     8221 ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt
     8281 attggtctac cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc
     8341 ccgaacacca cttatgtgtt taaggctggt gaagaagcgg tgccgctaga agctggtcac
     8401 cgtttttaag taaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga
     8461 cgcttgacgc gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc
     8521 atcttcatcc ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg
     8581 ctgcatatct cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg
     8641 ctgatgcagg cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg
     8701 tttgctgcga cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc
     8761 cagctttaaa ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag
     8821 cgaaaatgcc cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac
     8881 gatatcggaa gcctgttcaa ggttgagcga catttcaata atctccgccc agcgacgcga
     8941 ctcttcttct gccagttcct cttttggcat ccgcgccaga tacagcttaa tggcggtata
     9001 gagaacgttg atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg
     9061 catcacttta ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc
     9121 gcgcgcggcg ttcgccagag caagcgtggg cgtatccagc gcgctgacat ccagatgttt
     9181 aggccgtagc tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc
     9241 catcgggtca acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa
     9301 atagatcacc agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg
     9361 gacaaacggc aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac
     9421 acggcgggct gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag
     9481 gttagcacca atcaccagac agagcgccac ggggaaggag ataatgcctg cggcggtcag
     9541 agtcgcagtc agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc
     9601 aatcagcgca tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt
     9661 tgcctgggtg atcggcgtta cggcctgcac aatcaactcc agcgccagta aaatcagccc
     9721 aagaccaata ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa
     9781 aatcacgccg ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg
     9841 cgccattagc gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc
     9901 taccagatcc tgagcgacaa acgaggtcac cagcatggtg gtggcattac tgctctgcac
     9961 cagtgcggta acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct
    10021 aaggacagta cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt
    10081 cccccagacc agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg
    10141 ttctccttat cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct
    10201 gactggcatc tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct
    10261 gtttccagta taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt
    10321 tgttgtaagg aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc
    10381 cagccagcgc tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg
    10441 atcgcgctga atttgcgcca ccgcgtagta cttgctgtcc gggtggctga aataccaacc
    10501 cgaaaccgat gcaccgggcc acatggcgaa agattctgtg agtttcatgc cggtatgttt
    10561 ttccacttcc agcagctccc agatggtggc tttttccgta tgttccgggc aggccgggta
    10621 gcccggtgcc ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt
    10681 ctcgttcggc gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa
    10741 ggcttcggct aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg
    10801 cgcttcaaag gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc
    10861 gatgtaatct gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc
    10921 gaatcccgtt ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg
    10981 cgtttcgtca cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac
    11041 cacgccacgc ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc
    11101 tttaaacagc cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt
    11161 cccggccagc gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagagtttc
    11221 gatgctggct tcaacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg
    11281 ccagtcaaaa gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgcgg
    11341 tttcttacgc ccgtgctgaa tacgtacggt ttcgtactcc ttgcgggtac gagcgacaaa
    11401 atcatcacgc tgagtatcgg aaagcagcgc cgccaccaca ccaacggtgc gcgatgcgtt
    11461 ctgtacatac accgtcgggc cgctgtagtt ctgctcaatt ttcaccgccg tgtgcgcttt
    11521 tgaggtcgtc gcgcctccaa tcaatagcgg gatggtgaag ccctgacgct ccatctcttt
    11581 cgccacgtta accatctcgt ccagcgacgg cgtgataagc cccgaaagtc caatcagatc
    11641 agcattcact tctttagcgg tacggagaat tttttccgca ggcaccataa cgccgagatc
    11701 gacaatttcg tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg
    11761 gacgtcgccc ttcacggtgg cgatcaccat cttgccgttg gttttgccct gctctttgct
    11821 ggcttcaata aacggttcga gatacgccac cgcctgtttc atgacgcgcg ccgatttgac
    11881 cacctgtggc aggaacattt tcccttcgcc aaacagatcg ccgaccacgt tcatgccgtc
    11941 catcaacggg ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcggt
    12001 atcctgctcg ataaactcgg taatgccttt gaccagcgag tattccagac gtttattcac
    12061 ttcccacgag cgccactccg cctgctgggc gttagcggtg tcgtcggttt tgctgccgcg
    12121 atatttctcg gcaagctcca gtaaacgctc agtgccatca tcgcgacgat taagaatcac
    12181 atcttccacc gcgtcgcgca gttcagcggg caggtcgtcg taaatcgcca gctgcccggc
    12241 attgacgatc cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat
    12301 ggcttcgcgc accggatcgt tgccacggaa cgagaaagaa acgttagata cgccgccgga
    12361 aattagcgcg tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc
    12421 gtagttgttg tgctcttcaa tgccggtggc aaccgcgaag atgttcgggt cgaagatgat
    12481 atcttctggc gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc
    12541 gattttccgt gcgcgggtat cggcctgtcc ctgttcgtca aaggccatta ccaccactgc
    12601 cgcaccgtaa cgacgcaaga gcttcgcgtg atggataaag gcgtcgacgc cctctttcat
    12661 cgagatagag ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc
    12721 ccattttgag gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag
    12781 attgagaaaa cgcaccatcg ccgcttcggc gtcgagcatc ccttcatcca tgttgatatc
    12841 gataatctgc gcaccgtttt ccacctgctg gcgggccaca tccagtgcct cgctgtattt
    12901 ctcttctttg atcaggcgct tgaacttagc ggaaccggtg acgttggtgc gttcacccac
    12961 gttcacaaac aggctatctt cgccaatgtt cagcggctcc aggccggaca aacggcaggc
    13021 tacggggatt tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc
    13081 aatatgttgt ggcgtggtgc cacagcagcc gccgacgata ttgagaaaac ccgcttgcgc
    13141 ccattcacgt atctgttttg ccatcgtgtc ggcgtcgaga tcgtactcac caaaggcgtt
    13201 gggtagcccg gcgttcgggt gcgcggtgac gtagcattcc gcaatccgtg acagctcctg
    13261 cacgtactgg cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagagcttc
    13321 ggcgtggcgc aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga
    13381 ggcgtcggtg atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc
    13441 cgttttcacc gcaaaaaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag
    13501 gatcagatcc gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccaccag
    13561 ctggtcaaaa gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt
    13621 gcggttggtt gggccgagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt
    13681 ccactcgtca gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag
    13741 ggattccatc tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat
    13801 gatatccgcg cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttgct
    13861 gagcaccagc aggtcgttgt tgcctttgag atcgcatggc cagtcggcaa agcgttcacc
    13921 acgaaaatcg gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccag
    13981 caccagaata cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc
    14041 tcccgacacg ctcaacccag atttaataaa aattcaacaa accatactgg cataaacgca
    14101 tctgtggtaa aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc
    14161 agttcagcaa ctattgcatt agctaacaat aaaaatgaaa atgatttcca cgatacagaa
    14221 aaaggaggct gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc
    14281 caccgcacca gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg
    14341 gattgccgaa tccaatggca gtgtggcact cacagagctg gcgcaacaag ccgggttacc
    14401 caattccacg acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt
    14461 cggcgaactg ggacattggg caatcggcgc acatgccttt atggtcggca gcagctttct
    14521 ccagagccgt aatttgttag cgattgttca ccctatcctg cgcaatttaa tggaagagtc
    14581 tggcgaaacg gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga
    14641 ccaggtacag tgtacgcatc tgatgcgaat gtccgcgcct atcggcggta aattgccgat
    14701 gcacgcttcc ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa
    14761 gctgctgcac cgcaaagggt tacatgccta tacccacgca acgctggtgt ctcctgtgca
    14821 tttaaaagaa gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca
    14881 tgcactgggg ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc
    14941 cgcaatttca atttccggac cgatttcacg tattaccgat gaccgcgtga ccgagtttgg
    15001 cgcgatggtg attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact
    15061 ttttctggcg ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg
    15121 tgtctgttaa tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat
    15181 taactcagca ttatttttaa acatcaaact acttaatcat taacaaaatc gcaagatctc
    15241 attccaccga aacttttacg gctgcattat gagattgagc gatttatgat atttctttag
    15301 ttgtaatcat attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac
    15361 aatgagatat ttaaatgatt actcgtatcc ctcgtagttc tttctctgca aatattaata
    15421 atacagccca gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg
    15481 ataaattttc tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta
    15541 gacaaaatgg tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg
    15601 tcttacaagc tgataagaaa gaaattacga taattaataa aaacgaaaac acgacactta
    15661 ctcaaaccat tgccccaata tttgaagaat acctaatgga aattttacct caacgctcag
    15721 acactcttga taaacaagaa ttaaacctaa aatcagatag aaaagaaaaa gaattcccaa
    15781 gaattaaact taatggtcaa tgttattttc cggggcgacc ccaaaaccgt atagtatgcc
    15841 gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc
    15901 aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc
    15961 agactttggc gttgatttcc agccgtcctg aggggcgttg tgttgctgcc tgtggtgatt
    16021 tcgggctagt tatgaaggca tatttcgaca agatggaatc aaatggcatc agtgttatgg
    16081 cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata aagaacacaa
    16141 ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtcact aacgataaaa
    16201 taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg gattttatga
    16261 atgtagatta tagcctcctg aaatggtcaa atgatcatat tattaaccaa tctgttgcaa
    16321 taattccagc acttccgaaa gaacagctat tgatgttaaa aggatctgtg gatgaaataa
    16381 cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag aatcaccaac
    16441 ttacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca gaaatgttga
    16501 ctgcctataa tagtggacat atgaacgtta ttaatactat ttttaacgca ttacccactc
    16561 tgtttaatac gtttaaattc gataaaaaaa atatgaagcc cctcctcctg gcaaataatt
    16621 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaat gttgtagaga
    16681 cggtttatct tgctttatct aaccatgcac gcctgtttgg atttaccgct gaagatatta
    16741 tggatttttg gcaacacaaa gccccacaaa aatactctgc ctttgagttg gcttttgaat
    16801 tgggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    16861 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    16921 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    16981 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17041 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17101 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17161 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17221 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17281 catgtcgcca ggaaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17341 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17401 ccgccgctcg atatagagat ggcgaattac aatctgctca ccaagatcgg tgattttttc
    17461 cgctgcttcc tggagcagta attccattaa tgccggggaa atatgccgct tctccagcac
    17521 aaagttttca aactcctggg tgtccgccat tcggcccaca cggtcgtgct ctttcaccag
    17581 ttgatagcag gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt
    17641 gatgactttg aatacccgat caaagcccgg cagcgtaaat accaacatca ccattccacg
    17701 aatacccggc gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg
    17761 gtagctttcg gttttggcgt gcttctggca gccgatagcc atatacaatt cagcagtggt
    17821 tttacccggc agaatttccc gtagccactc gaccagcgct gcgggcagcg gtgcataaac
    17881 cataaaataa gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca
    17941 ggtatcaata aataactcgc cgtcgttcgt ctggtggatc ggcagtaaga agggcaacgt
    18001 gccggaaggc gtgatcagtt tgcccaccag ccaggcggct ttattgcggt aaaacagttc
    18061 gttcgccacc tgtaaatgac tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg
    18121 gcgaatgatg taatgaatgt cacggctttt attctgccag cgcaggcgca gcggcaggtc
    18181 gctgataacg cgcatcagta gagattccca gccgtgatcg gggtgaaagt ctttcgccag
    18241 cgggcgggga atggtacgaa agcggcgctc tggctgagag ctaaagataa aaagccgctc
    18301 gggagtaagc gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc
    18361 aatctcgaag cgcgggtaat ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa
    18421 aaatgccgcg tccgtacttt ggccgttagt aatgcagcgc agttgctcca cgaccagacc
    18481 aacgtgatga tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc
    18541 ggcctgttcg aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc
    18601 gtcgaagcct tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg
    18661 ccctcatcag gagcagagaa ttgccggatg cggcgtaaac gccttatcca gcctacgttc
    18721 ggcaacggtt gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt
    18781 gccggatgcg gcgtaaatgc cttatccggc ctacgttcgg caacggctgt aggcatgata
    18841 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtgaacgcct
    18901 tatccggcct acagtcagca acggttgttg ttgcttagaa ctgcgattct tcagtggagc
    18961 cggtcagcgc ggtgactgaa gacgtgccgc cctgaataat agtcgtcact ttatcgaagt
    19021 aacctgtacc cacttcctgc tggtgagata cgaaggtata gccatctttc gcggcggcaa
    19081 attccggctg ctgcactttc tcaacgtagt gcttcatacc ctcgccctgg gcataggcgt
    19141 ttgccaggtc aaacatgttg aaccacatgc tgtggatacc tgccagggtg atgaactgga
    19201 acttgtagcc catatccgac agctgctgct ggaagctggc aatagttttg tcgtcgaggt
    19261 ttttctgcca gttgaacgac ggcgagcagt tataagccag cagtttgccc ggatatttcg
    19321 cgtggatagc ttgtgcaaag cgacgcgcca gttccagatc cggcgtggag gtttcacacc
    19381 agaccaggtc agcatatggc gcatacgcca ggccacggct gatcgcttgc tcaatgcccg
    19441 catgagtacg gaagaagcct tcactggtac gctcgccagt aataaattca ctgtcatacg
    19501 ggtcgcaatc ggaggtaatc agatccgccg catcggcatc ggtacgagca accagcaggg
    19561 ttggaacgcc cgtcacgtca gctgcaagac gcgccgcgac cagtttctga atagcttcct
    19621 gagttggcac caaaactttg ccgcccatgt gaccgcattt cttcactgac gccagctgat
    19681 cttcgaagtg aactgccgct gcaccggctt caatcatcgc tttcatcagt tcaaaggcat
    19741 tcaggacacc gccaaaaccg gcttccgcat cggcaacgat cggcaggaag taatcgacat
    19801 agcgcggatc gcccggctca atgcctgcgg accattggat ctgatcggca cgacggaagg
    19861 tgttgttgat ccgctccacc acagctggca ccgagtttgc cggatagagc gactgatccg
    19921 gatacatgct ggccgccagg ttagcgtccg ccgctacctg ccatcccgac agatagactg
    19981 cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca ccgaggctgt
    20041 tgatgtagcc ttttttcgac tcgccatgca gcagacgcca cattttcgcc gcgcccagtt
    20101 gcgccagggt gcattcagga ttgactgaac cgcgtaattt caccacatct tccgcgctgt
    20161 atgggcgagt aatgccttcc caacgcggtt gagtccactc tttctgtaat tcttcaattt
    20221 gttgtgtacg ggttttcatg tgcagatgct ccatagttat gtggtggttt acgctaacag
    20281 gcggtagcct ggcagggtca ggaaatcaat taactcatcg gaagtggtga tctgttccat
    20341 caagcgtgcg gcatcgtcaa aacgcccctg ggagaaacgt tcttcgccca gttcgctggc
    20401 aatgactttc atctcttcac ccagcatctg gcggaacaag gctttggtca ccggtttgcc
    20461 attgctcaac gttttttgat gatggatcca ctgccagatt gaggtacggg aaatttcagc
    20521 cgtcgccgca tcttccatca ggccataaat cggcacgcag ccgttgccgg agatccatgc
    20581 ttcgatgtac tgcactgcca cgcgaatgtt ggcgcgcata ccttcttcgg tgcgttcacc
    20641 atcacaaggt gccagcagct gatcggcagt aatcggcgcg tcttgttcgc gcatcacttc
    20701 aagctgattt ttacgggagc cgagaatgtc gttgaatacc gccattgccg tatccgcaag
    20761 gcctgggtga gcaatccatg tgccatcgtg accgttattg gcttccagcg ctttatccgc
    20821 ttttactttg ttgagcacct ggttattgcg ctcttcatct ttgctcggaa taaacgctgc
    20881 catgccgccc atcgcaaaag caccgcgttt atggcaggtt ttaatcaaca ggcgtgagta
    20941 agcattcagg aatggtttat ccatcgtcac tgcctgtctg tctggcagga cgcgatcggg
    21001 atagtttttc aacgttttga tatagctgaa gatgtaatcc caacgaccgc agttcagacc
    21061 aacaatatgg tcacgcagcg cgtgaaggat ttcatccatc tggaacacgg cgggcagcgt
    21121 ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata
    21181 gctgaagact tcgctccacc aggccgcttc ctgccaggac tgggttttcg gcagatagaa
    21241 atagggaccg ctgccctttg ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa
    21301 atcaaacagg ctaccgggga ttgcctcacc acgccaggtg acatgttttt ccggcaagtg
    21361 cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc ttgagctgat aaattttgcc
    21421 tgcttcattg gtatagctga tggtgccgtt aaccgcatcg cgcaggttaa tttgcccgtc
    21481 gatcactttg ttccagtctg gtgccagtga atcttcgaaa tcggccataa agactttcac
    21541 attggcgttc agtgcgttga tcaccatctt gcgctcaacc gggccagtta tctcgactcg
    21601 acgatcttgt aagtccgcgg gaataccacg aattttccag tccgtatcgc gaatggaagc
    21661 tgtttccgaa ataaaatcag gcaaagttcc gttatcaata tcttgctgct gctgaatgcg
    21721 cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc accagctcag tcagaaattc
    21781 taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca tacggcctta tgaaagccag
    21841 ttcgtcggtt gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg acccgaaaac
    21901 ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt cagcacaact aagactactc
    21961 atttaaaatg caaaat
//
LOCUS       DAZUFB010000078        21973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZUFB010000078 DAZUFB010000000
VERSION     DAZUFB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.078"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..212
                     /locus_tag="ACHY3M_003319"
     CDS             <1..212
                     /locus_tag="ACHY3M_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4318172.1"
                     /translation="WISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDWSRNKWWAI
                     TDDGEEPASKVTHWMTLPEPPQEVK"
     gene            212..427
                     /locus_tag="ACHY3M_003320"
     CDS             212..427
                     /locus_tag="ACHY3M_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4318173.1"
                     /translation="MNNLMTTKQVADFCGVSVSTVLRWNSVNRRTGQKYRPDFPDPDI
                     KSCPNKWASRKIYKFAGVIEECEAAQI"
     gene            658..1884
                     /gene="dgt"
                     /locus_tag="ACHY3M_003321"
     CDS             658..1884
                     /gene="dgt"
                     /locus_tag="ACHY3M_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFS4318174.1"
                     /translation="MIDNEVKIFAKEKEKMLCNKLSFYREHAQSSEDTRKRNEFERDY
                     ARILYSSSFRRLQGKMQLFEVDPQKFNRNRLTHSLEVAQIARSIASELELEHPVVAEL
                     AALAHDIGNPPFGHSGEKKLNDIANDFGGYEGNAQALRILRSLEIKHPNCPGLNLTHR
                     AILSVVKYPYKKENGRKKYLYNDDYKYYTELVNRYELDLQTGEKTIDAQIMDLSDEIA
                     YAAHDLEDALSRSMVSIEDIFYEFGISKYKDSIDLLNKIIEKSKYTASRASRLNSSEE
                     FAIIFRKELTSNIVNALVNDITVIQSENGFKQLGFSTTKNLSAGLKDIVFKVIMRKRD
                     IKSYEHKGNIIIEDLFNFYNYRDNIEFISPQLYRTLPNKINPIYQELKKRAIVDYISG
                     MMDTFAIREWETHHKK"
     gene            complement(2256..3479)
                     /locus_tag="ACHY3M_003322"
     CDS             complement(2256..3479)
                     /locus_tag="ACHY3M_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4318175.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKAPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQVWW
                     ERVESLVTCPSSDSS"
     gene            complement(3864..4541)
                     /gene="yjjG"
                     /locus_tag="ACHY3M_003323"
     CDS             complement(3864..4541)
                     /gene="yjjG"
                     /locus_tag="ACHY3M_003323"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFS4318176.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(4556..5002)
                     /gene="rimI"
                     /locus_tag="ACHY3M_003324"
     CDS             complement(4556..5002)
                     /gene="rimI"
                     /locus_tag="ACHY3M_003324"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4318177.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGECYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(4971..5384)
                     /gene="holD"
                     /locus_tag="ACHY3M_003325"
     CDS             complement(4971..5384)
                     /gene="holD"
                     /locus_tag="ACHY3M_003325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFS4318178.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            5487..6518
                     /gene="rsmC"
                     /locus_tag="ACHY3M_003326"
     CDS             5487..6518
                     /gene="rsmC"
                     /locus_tag="ACHY3M_003326"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFS4318179.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            6786..6872
                     /locus_tag="ACHY3M_003327"
     tRNA            6786..6872
                     /locus_tag="ACHY3M_003327"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6820..6822,aa:Leu,seq:cag)
     gene            complement(6911..7147)
                     /gene="yjjZ"
                     /locus_tag="ACHY3M_003328"
     CDS             complement(6911..7147)
                     /gene="yjjZ"
                     /locus_tag="ACHY3M_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFS4318180.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            7288..8076
                     /gene="fhuF"
                     /locus_tag="ACHY3M_003329"
     CDS             7288..8076
                     /gene="fhuF"
                     /locus_tag="ACHY3M_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFS4318181.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(8114..8791)
                     /gene="bglJ"
                     /locus_tag="ACHY3M_003330"
     CDS             complement(8114..8791)
                     /gene="bglJ"
                     /locus_tag="ACHY3M_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFS4318182.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHSY"
     gene            complement(8749..9474)
                     /gene="yjjQ"
                     /locus_tag="ACHY3M_003331"
     CDS             complement(8749..9474)
                     /gene="yjjQ"
                     /locus_tag="ACHY3M_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4318183.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            10093..10863
                     /gene="yjjP"
                     /locus_tag="ACHY3M_003332"
     CDS             10093..10863
                     /gene="yjjP"
                     /locus_tag="ACHY3M_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFS4318184.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            10854..11327
                     /gene="yjjB"
                     /locus_tag="ACHY3M_003333"
     CDS             10854..11327
                     /gene="yjjB"
                     /locus_tag="ACHY3M_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFS4318185.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            11434..11973
                     /gene="dnaT"
                     /locus_tag="ACHY3M_003334"
     CDS             11434..11973
                     /gene="dnaT"
                     /locus_tag="ACHY3M_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFS4318186.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            11976..12713
                     /gene="dnaC"
                     /locus_tag="ACHY3M_003335"
     CDS             11976..12713
                     /gene="dnaC"
                     /locus_tag="ACHY3M_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4318187.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            12762..13256
                     /gene="yjjA"
                     /locus_tag="ACHY3M_003336"
     CDS             12762..13256
                     /gene="yjjA"
                     /locus_tag="ACHY3M_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFS4318188.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            13510..15801
                     /gene="opgB"
                     /locus_tag="ACHY3M_003337"
     CDS             13510..15801
                     /gene="opgB"
                     /locus_tag="ACHY3M_003337"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFS4318189.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(15941..16963)
                     /gene="lgoD"
                     /locus_tag="ACHY3M_003338"
     CDS             complement(15941..16963)
                     /gene="lgoD"
                     /locus_tag="ACHY3M_003338"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFS4318190.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            17102..18016
                     /gene="lgoR"
                     /locus_tag="ACHY3M_003339"
     CDS             17102..18016
                     /gene="lgoR"
                     /locus_tag="ACHY3M_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS4318191.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     APLALRNHLNSAKQSMIRSINENTRYAH"
     gene            18231..19592
                     /gene="lgoT"
                     /locus_tag="ACHY3M_003340"
     CDS             18231..19592
                     /gene="lgoT"
                     /locus_tag="ACHY3M_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318192.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPYGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(19641..21305)
                     /gene="tsr"
                     /locus_tag="ACHY3M_003341"
     CDS             complement(19641..21305)
                     /gene="tsr"
                     /locus_tag="ACHY3M_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFS4318193.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TKAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(21424..21870)
                     /gene="hpaR"
                     /locus_tag="ACHY3M_003342"
     CDS             complement(21424..21870)
                     /gene="hpaR"
                     /locus_tag="ACHY3M_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFS4318194.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         5667 a         5556 c         5140 g         5610 t
ORIGIN      
        1 gttggataag ctgtagtgag cgaatgccga acgataaaca gtatgtttgg tgttggggga
       61 agtcttacgg ctggactgag tgcgatacct tcgaagggta ttacgattgg tcgagaaaca
      121 aatggtgggc aattactgac gatggggaag aaccggcatc gaaagtaacc cactggatga
      181 cgctaccgga gccaccgcag gaggtgaagt aatgaacaac ttaatgacaa ctaaacaagt
      241 cgccgacttc tgtggcgttt cagtatcgac cgttcttcgc tggaacagtg taaacaggag
      301 aactggccag aaatacaggc cagactttcc agatcctgat attaaatcct gcccaaataa
      361 atgggcatca cgtaagattt acaaatttgc cggagttatt gaggaatgtg aagcagctca
      421 gatatgagat gacacatcct atagcacata gccaaacata gcaatcagac tgtccgcttt
      481 gtgttgaagc ggacattgcc gcagcgactt gtcttagcca taaataacca aaacgatgcg
      541 ctgttcatat aaagttcttt taaacttcaa atcagtaatt tttgtctaaa tcttttcaaa
      601 ctcataaaaa ccagtaagat aagaaatcac ctttacctgt ttttatgaga ggcataaatg
      661 attgataatg aagttaaaat ttttgcaaaa gaaaaagaaa aaatgctgtg caacaaactt
      721 tctttttaca gggagcatgc tcaatcgagt gaagatacaa gaaaaagaaa cgaatttgaa
      781 cgagactatg cgcgtattct ttattcctct tcgttccgcc ggttacaggg gaaaatgcag
      841 ctctttgaag tcgatcctca aaaatttaac cgtaatagac tgactcatag cctagaagtt
      901 gcccagatag cccgaagcat cgcatccgaa ctagaacttg aacatcctgt tgtagcagaa
      961 ctggctgcac ttgctcacga tattggcaac cctccgtttg gtcattccgg tgaaaaaaag
     1021 ctaaacgata ttgctaatga tttcggcggc tacgaaggca atgcacaggc acttagaata
     1081 ctcagaagtc tcgagataaa gcatccaaat tgccccggtc tcaaccttac ccaccgtgcc
     1141 attctctccg tcgttaaata tccttataaa aaggaaaacg gcagaaaaaa atatctttat
     1201 aatgatgact ataaatacta cacagaattg gtaaatagat atgaattaga tttgcagact
     1261 ggcgagaaga ccattgatgc acagataatg gatctgtcag acgaaattgc atatgccgcg
     1321 catgaccttg aagatgcact aagccgtagc atggtcagta ttgaagatat tttttatgag
     1381 tttgggatat ccaagtacaa agactcaatt gatttattaa ataaaatcat tgaaaaatct
     1441 aaatatacag cttcgagagc atcaagactc aattcatctg aagagttcgc cataatattc
     1501 agaaaggaat tgacatccaa tattgttaat gcgcttgtta atgatattac tgttattcaa
     1561 agtgaaaatg gattcaagca acttggattt agcactacaa aaaatctgtc tgcaggcctt
     1621 aaagatatcg tatttaaagt catcatgcga aaaagagata ttaaaagtta tgagcacaaa
     1681 ggaaacatta tcatcgaaga tttatttaat ttttataatt atagagataa catagaattc
     1741 atatcccctc aactgtatag aacgctccca aataaaataa atcctattta tcaagaactt
     1801 aaaaaacgtg caattgtcga ctacatctct ggaatgatgg atacatttgc catccgcgaa
     1861 tgggaaacgc atcacaaaaa ataaccaaac tggcgcacat caccaacaga ttcagtgcca
     1921 ctcttcccat gggacagaac agtgattctg tccattatca cccgattata cagataagcc
     1981 acatctagta gttctgagct agcattttcc acattgatta cgcttccgtg cacatcgtta
     2041 tttttaacca aaaagaaagg acatatgcaa aaataatatc aagtaacgga gtgtgttaaa
     2101 tcgtaaccaa tcatctttac tttacatttc gtaacttaaa ccgtacgttt tctaaacatt
     2161 aaatatacac tttgcgctgt aagttgtatc tctccgttaa gctcactgct caacgccaat
     2221 agtcatattt tattgcgttc tggctgcgca aattgtcaac tggaatctga actaggacaa
     2281 gtaacgagcg attcaaccct ctcccaccat acctggtagg ctttacgctg ttcttctaga
     2341 taatcgctct tgtcataaac ctgccatacc cctggcagct tatgaccaag cataatttct
     2401 gcaatatgag gcgcagtaag atcagaaaag tttgttcgtg ctgttcgcct caaatcatga
     2461 agagaccaat gagggaattg ataccccaaa cgtcgccatg cgaactgcat taaattgtaa
     2521 ggcagcgact gcaatgatgt ccgaccaact ggctccctgc ttccttcctt agtaaaaagc
     2581 atatcggaac cgttgttcat agagataacg tattttataa gctcttcaac cggttcaata
     2641 atgggccgct ttagtggttc gcctgttatc tcccctgtct tatgtcgttc tggcggtaca
     2701 gtccatacct tattaatgaa atcaaaatcg tccaccctgg cggtaattag ctctgaacta
     2761 cggcagccaa aatgcagcaa tagtttaatg aaggcccggt atttaggaac cattcgagaa
     2821 ccatcgatcg cagcataaag gattttaatt tcatcatgtg tcagaaaccg tttcttctga
     2881 cctttacgga tatccatatc tttacccgtg atatccgaca gcgggcgagt ttcaatgagc
     2941 tttctcttat acgcccagac atgggcctgc tttgcgttaa ttagtaatcg gtctgctatt
     3001 gctggagcct tagtgctaag aggctccagg acttctaacc aatcatgcaa tgtagctgca
     3061 tcgtgaggga tattcccgat tttagagaac aggtgcaact caaacgagcg gagtatctgt
     3121 tcagaaccct ttttattttt tacacaatat gcttcatacc aggcacggat cacagactct
     3181 accgtcatgg cttcagtagc ttttcgtttt tcagcctgct tgaccaatcg tggattacgg
     3241 tttgactcga gttcaccacg gagacggata acttcttctc tggcctcttt taatccagtt
     3301 gccgggtaag ttccgatatc aagacgctca cctttccctg cccattgata acgatattgg
     3361 aatactacgc gacctttcgg tgatactctg acagacagac catcacgatc ggatttaacc
     3421 aaaaccttat cacgttcctt tccaacgact gaacgcaacc acgcatcaga cagcgccatt
     3481 actcaccttg tacaaatcgc aaaacacctc tgctgctttg tacattatgt acaagcatat
     3541 gtacagattt ttgatgaaag caacctgatc gatttaaagt tacatgaaag aatcttcaat
     3601 taaaaagtca cacaaatata tgtatttaaa tgatttatat gaccaaaact caattaactg
     3661 aaaggatttt gaaagagtaa aagagcttta atttgggctt aattattggg gtgagaaata
     3721 atggcaaaag tgcggcgctt cgccacctct tgcaaataag gagacaacgt cataattctt
     3781 tcttcttgag taagcggcat cgcgccgcgc ttgttgaaaa cgaaaaattg cggctatttt
     3841 acccatcaac gggggggagg caatcagtgt ttacacagga gctgctccag ttcgtgcaac
     3901 gatgaaacgg tccaggtggg cgcgatgcct tctggttgct cgcgattgtg cgcattcagc
     3961 cagcaggtcg caagcccggc gttgatgcca ccgagaatat cggactcagc agtgtcgcca
     4021 accatcagca cgcgtgaacg gtcaggattg cccgcctgtt ccagcgcata atcgaaaatt
     4081 ttcttattcg gtttggcaac gccaacttct tcggaaatca ccagcaaatc gaagtaatca
     4141 cgcaggcccg tgcgttccag acgcacttgc tgcaaggcgc taaagccgtt ggtgatgatg
     4201 ccgattttgg cgttgccacg aatggcgtta agcagagaaa ccgcgcccgg cagcggcgtg
     4261 cagatttccg ccatcgcatt aataaaggca tcgttaagca agcctgcttc aactttcagc
     4321 cgttcggccc agctctcgaa acgcccgtgc tgaagctgta atgaagtgat cgcgccgttt
     4381 tgataatcca cccacagtgg tttgttaacg gcctgatagt cctgaaaatc ttcagcggta
     4441 aaggtgacgc tgtaatcaag aaacatccgc tgcaggccgg tgaatgagtc aaaggtaaac
     4501 agcgtttcat cggcatcaaa gaaaatccag tcccacttca ttattccacc ttgtcttaca
     4561 tactgattgg caacgccatg atgatggcgt cttcgcgacc gtccgtggtg gggtagtaat
     4621 tgcggcgaat cgtcgcctcg ttaaagccta aactttcgta cagggcaatg gcggcagcat
     4681 ttgaggcacg gacttccagc cagagagtcg ccacgccgcg tttttccagt tcgtcgatca
     4741 gatgttccag cagcaccctt cccaatccct gacgctgata gtcaggatcg accgcaatat
     4801 tgaacaatgt agcttcatcc agcaccactt gcgtaatcgc aaacgccgcc attttgccgt
     4861 tttgcgttaa ctgaaagttg agataacact cgccctggtt gctggcaaag gttttttcac
     4921 tccacggaaa ggcgtgggcg cgttgttcaa tgtggtaagc cgctggtaaa tcagtcgttt
     4981 cgagggaaga aatcgtgttc atatgtgcaa atctgttgcc ataacgcggc gcgtgccgtt
     5041 gggtttgccc gtaattcggt gagcgccggt gatgccacct gagcgccttc cagtgatagc
     5101 ggttcgtcag tacccaaccg ccagctgttg cagcgactgc cttgcggcag catcgcgatt
     5161 ttttctggcg tcagttgcag cacttgatcg gggctgacgg ttaatgcgcg cagaacatcg
     5221 ctcactaaag gatcggtcag ggcgggaaga tcgtttgcca ccatcaccag acggacgtgt
     5281 gccgggatcg caatggcaat ctccccctgc aacgcgccag ggcgacgcag cgaccactgg
     5341 gtaatgccca gttgctgtaa ctgccagtct cgtcgggatg tcatagcgaa tcgctcctgt
     5401 tgtcaggggc gcaaatatag caaattcgtc gataccgcgc caacatatgg ctataatcgc
     5461 cgccagtatc aattgaggag cattccatgt ctgcatttac cccggcaagt gaagtcttgc
     5521 tgcgtcacag tgatgatttc gaacaaagcc gtattctgtt tgccggagac ttacaggatg
     5581 acctgcccgc gcgtttagat accgcggcca gccgtgctca tacccagcaa ttccaccact
     5641 ggcaggtatt aagccgccag atgggggata acgcccgttt cagtctggtc gccacggtgg
     5701 atgacgtcgc agattgcgat acgctgattt actactggcc gaagaacaaa ccggaagccc
     5761 agttccagtt gatgaattta ctttctctgc tgccagtggg gacagatata tttgtcgttg
     5821 gcgagaaccg cagcggcgtg cgcagcgccg agcagatgct ggcagattat gcgccgttga
     5881 ataaagtcga cagcgctcgt cgctgtggcc tctattttgg tcgtctggaa aaacagccgg
     5941 tatttgatgc cgataaattc tggggcgaat acagcgtcga tggcctgacg gtcaaaacgc
     6001 tgcctggcgt gtttagccgc gacggtctgg atgtcggtag ccagttgctg ctctcgacgt
     6061 taactccgca cacgaaaggt aaagtgctgg atgtcggctg tggcgcgggg gtgctttcag
     6121 ttgcctttgc gcgccattcg ccgaaaattc gtctcacctt gtgcgatgtc tctgcgccag
     6181 cggtagaagc cagccgcgca acacttgcgg ccaactgtgt tgaaggtgaa gtctttgcca
     6241 gcaacgtctt ttccgaggtg aaaggtcgtt ttgatatgat catctccaac ccgccgttcc
     6301 acgatgggat gcaaaccagc ctggatgcgg cgcaaacgct gattcgcggc gcggtgcgtc
     6361 atcttaatag cggcggcgag ctgcgaattg tagcgaacgc cttcctgcct tacccggacg
     6421 tgctggatga gacatttggc ttccatgaag tgattgcgca aaccgggcgt ttcaaggtgt
     6481 atcgcgccat tatgacccgc caggcgaaga aaggttaatt atctcgccga ataccgtgtc
     6541 ggatgcggcg tgaacgcctt atccgaccta caaaacctgc acgttagccc ttcgtaggcc
     6601 agataagacg cgccagcgtc gcatctggca tctgcccccg catgcctacc aaacctgcac
     6661 gctagtttcc tgatggacat ttttccagca attacacctc cggcgataat taactattga
     6721 cgaaaagctg aaaaccacta gaatgcgcct ccgtggtagc aattcttttt aagaattgat
     6781 ggtatgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg
     6841 acgtgggggt tcaagtcccc cccctcgcac caattatctt tacttccttt cttgtttctt
     6901 ccttgatttt tcatccctga ttcaaattca ttatcatcag catgatggca gcaataagcg
     6961 ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt gtgatacagc atcaccgacg
     7021 tcaatactaa tcccatcaga atgacaatcc gccactgatc cggtgataaa tccgcataca
     7081 tcagccctgc aatcagcaat cctggcagca acactcccca gccactgccc agcgtacgtt
     7141 gcaacatggt ttcatctctt tcattgataa tgataaccaa tatcatatga taatttttat
     7201 catttgcaag ctagagaaat cccttgctat cgggtaaacc tatcgctagg attagcaatc
     7261 attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct ctatgaagat
     7321 gttatctggc gaacgcatct ccagccacag gatgctgggc ttgcacaagc cgtacgtgcg
     7381 acgatcgccg aacatcgtga acatttgctg gagtttatcc gcctggatga acccgctccg
     7441 cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactcgcg
     7501 gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg
     7561 atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta
     7621 ctgacgcagg aaaaggcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa
     7681 accggccgtg ccgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat
     7741 tcgccgcagc agcgaatgga aaggttaatc agccaggcgc tggttccggt tgtgcaggca
     7801 ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc
     7861 aactggtatc ttactgagat gaagcaactg ctcggcgagg cgacggttga atcgctgcgc
     7921 catgccctct tttttgagaa aacgcttact accggtgaag ataatccact ctggcgtacc
     7981 gtggtgctac gcgacggcct gctggtgcgc cggacctgct gccagcgtta tcgcttaccg
     8041 gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc
     8101 ggcatcatgc ggattaatag gaatgcaaca cattacttgc ttcgcaatgc cgcatacata
     8161 acagaatatc tgcggcctcc aacaaccctg catcagaact gacgcccagt ttcgacatca
     8221 cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg gcaatttgtg
     8281 tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg cttagcgtcc
     8341 ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta
     8401 atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt
     8461 ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca tccgcaataa
     8521 ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat aatccctctc
     8581 tgcgctcact tctcatggca gaaaaagaaa aaatgaccgc cgcaaatgag atccgcgaca
     8641 tggcatcctg aaatgactcc tgactggtat aggtatggag cttataaggg ttacccgcaa
     8701 actttctgaa taaactctca ataccgatac tgctcatgac gcatttttct atgagtgcga
     8761 catttctctt cttaattcgg ctgtgttcca ttctgcggca tctccataaa cagaaggcaa
     8821 gttaagttct cttgcaccct gcgcgctatt tatccatgca tacatttcag cgttgctgtg
     8881 aattgccagt cgacgcatcg cactgttttt ttgggcgctg atagttttat tacttttctt
     8941 aagcaacgaa gctatctggt tgatgcccca tcctttacct aatagccgca gtaccttgcg
     9001 ctctgaaaga gtgagaatga cggagcgata gctaaaatca tgagtttcat tcattgccgg
     9061 agaggtcagc atggcgctga tacgctctgc gtgcgtattg ccggaacgta cggtcttgac
     9121 cagattctca atgggttcaa catcagacaa taacgtcgcc gtagggcaca tgagcaattc
     9181 tactgcctgg gaataccagg agcgtgacac catgaaaacc cagtgaattt cccgatattg
     9241 tgagattaaa gaataataat gttcacaaac agaacgagga tcttcacttt caccggctaa
     9301 atcagcaatg actaatccgg aacgacgtaa ttgtaataag gtaaggtcct ccgcagaggc
     9361 gctggatatt atttcatatt cagggaagtg agtcctcatg acctctttta accctgcttg
     9421 cataacaggt attttactga taacaattcc atttttgcag catcctggca acatatttcc
     9481 tccacatcca ctgtctaaaa atcatgatgg tttattcagg atattcattc cttaaatgtt
     9541 tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt catatcagca
     9601 tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca aatgataacc
     9661 tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagga atacttaatg
     9721 aaaaacagaa atcacattca tttaatttat aattaaatga tatcccttcc cgcagaatga
     9781 aaaatcacac aaccaatatt tattcaatga aatctgatgg ttttaacttg ttaacattaa
     9841 aaaacgtgcc cggttatcta ttatcctgat tcgtaaaggc caaacaataa attcattata
     9901 gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga
     9961 gcttgtcccg ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa
    10021 ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac
    10081 aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt
    10141 ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga
    10201 ctgggtcggg cgttggggat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg
    10261 ctgacaacca ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc
    10321 attaatatgc atgtggtgac tgaagtccag cacattgtga ttctggcgga gcaccatctg
    10381 ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga
    10441 tggctggtag ccttaatggt tggcctttct tgtgcctgtt tctgtaaact caataaaggt
    10501 ggctgggatg gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag
    10561 ctgctggcac aacgtcatct tcatccacaa atcaactttt gccttaccgc tttcgccgcc
    10621 accaccattt ccggattgct tttgcaactt cccactttca gcaatacacc cactatcgcg
    10681 atggccgcca gcgttctgct gctggtgccg ggatttccgt tgattaatgc cgtcgccgat
    10741 atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca
    10801 ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg
    10861 tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg
    10921 gctttgcgat ggtgttcaac gttcccgttc gggcgttacg ctggtgtgcg ctgcttggcg
    10981 cgattggtca tggttcacga atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa
    11041 cttttatggc ttctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg
    11101 cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt
    11161 ataccgcaat gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga
    11221 ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc
    11281 tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt
    11341 aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc
    11401 ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac
    11461 gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc
    11521 ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc
    11581 ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg
    11641 gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtccccat ggggaaattc
    11701 gccatgtatc cggactggca acccgatgcc gactttatcc gcctggcggc gctatggggc
    11761 gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag
    11821 gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa
    11881 atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct
    11941 gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc
    12001 aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc
    12061 tactggcctg gcagaaagaa caaggagcga tccgctccgc cgctctcgaa cgtgaaaatc
    12121 gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcggccactg catcagaact
    12181 gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc
    12241 gccagtacgt cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag
    12301 gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat
    12361 ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata
    12421 gcggtaccag tgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg
    12481 atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg
    12541 atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga
    12601 tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg
    12661 tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt
    12721 ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac
    12781 atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt
    12841 cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac
    12901 aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct
    12961 taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc
    13021 tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga
    13081 acagtgaaga gcaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa
    13141 ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg
    13201 tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg
    13261 cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg
    13321 ctcctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta
    13381 taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac
    13441 gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag
    13501 gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac
    13561 gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta
    13621 tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac
    13681 gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc
    13741 ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc
    13801 ctgcgccgtc gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg
    13861 gcgctgggtt cggtggacgc cagcccagca tttcgtcaga taacggaact ggtgaaatcc
    13921 cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc
    13981 cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt
    14041 gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg
    14101 gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg catggtggct
    14161 tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc ctctgtctcc
    14221 agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac
    14281 tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat
    14341 ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtggccga cccgcactat
    14401 cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa
    14461 gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac
    14521 ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg
    14581 aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa
    14641 atcaaagcgt caccgtggtt taaagatacc gtcatcgtcg tctcttctga ccatttagcg
    14701 atgaacaaca cggcgtggaa ataccttaat aagcaggatc gcaataacct gttttttgtc
    14761 attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac
    14821 ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt
    14881 ttatccgggc agtcaatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg
    14941 aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac
    15001 cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg
    15061 gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc
    15121 cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctataagatg
    15181 gcacaactct gggctccgga actggcactc tccaccgact ggtgtgtctc gcaagggcag
    15241 cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa catggcaggg caaaacggca
    15301 tttaaagata cggtcatcga catggcgcgt tataaaggca atgtcgatac gctgaaaatt
    15361 gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca
    15421 gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac
    15481 gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac
    15541 ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta
    15601 ggcaatgaag aacaaactct tgtgctgggc aatgaagtga ccaccaccac actgcatttc
    15661 gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac
    15721 gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa
    15781 gttgtggaac gtgaagggta atttctgact gatgtgtagg tcgcataagg tgcgacctac
    15841 acatcttctc cggcctacaa aacatcattg agaaacagaa aaaaaacagc gcccaacact
    15901 tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta attacaccct
    15961 taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc gtggcgaacg
    16021 gatagcgatg ggttaacatc atgtcggcag tgatttttcc ttccgccatc agtcgaccga
    16081 ctttagcaaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt tcttttttat
    16141 ggaattccgg atcggagaac tgcaactcac ctttaaacag gccgacaaat accaccgtgc
    16201 cgccgtgacg aatcaaattc acggtgttat tcatcgcatg ttgattacct gtcgcgtcga
    16261 tcactttctg cgccagcgaa ccaccaaact gcgcccgtag ctgcgcgtca aaatcttcgg
    16321 ctgacgggtc cagtaaaggt aattccagac gcgttgccac atgttcacgg cgcgccggac
    16381 tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc gcgcccagac
    16441 cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct gcgcgacgca
    16501 ccgcatgagc gctaatagcg aaaggttcaa tcaatgcggc cgcctgcggg tcaataccgt
    16561 ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg ccatcctgat
    16621 gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta cacgccgggc
    16681 attgctgaca ggcaacataa gggatcacag caacttgctg accattttta agatcagcaa
    16741 tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaaca cgtggataac
    16801 taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc ccgacagact
    16861 taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt ttccagacta
    16921 attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg ctctccattt
    16981 tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa atctgttttg
    17041 tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca aggaggaaaa
    17101 tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg atgatatggc
    17161 gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa tgtacaacat
    17221 cagccgcaca acagtgcgtc atattctcag ccacttacgc gaatgcggcg tcttaacgca
    17281 ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt tcgcctgtac
    17341 taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca cgatgataaa
    17401 ccagcgtcag ttacgccctg gggaaacttt ttctgaactg caactggctc gggccgcggg
    17461 tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata atcttattca
    17521 aagcgaaaaa cgcggccagt ggagcatgaa gcaattcgat caatcctacg ccgagcagct
    17581 gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca acctgcctga
    17641 tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc tactgcgtga
    17701 caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc actccttact
    17761 gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct cagtcatctt
    17821 tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca ttgccgtcga
    17881 tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg ccccccttgc
    17941 gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta atgagaacac
    18001 gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg cacggtttta
    18061 tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt cgactaaaaa
    18121 tttttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg tcaaccttgc
    18181 gtaattcaca attacgtaaa caattaaaat tataaataca ggagtccggc gtggaaaaag
    18241 aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca gatattcccg
    18301 tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa accaccgcca
    18361 tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg ctgtcggtag
    18421 caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc gctttgctct
    18481 ccgtgttttc actcgcttac gggattgcgc aacttccttg cggcccacta ttggatcgta
    18541 aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc caggcaatgt
    18601 ctggcatggt gcacaacttt acgcagttcg tgttggtgcg tatcggtatg gggattggtg
    18661 aagcgccgat gaacccatac ggtgtaaaag tcattaacga ctggttcaac atcaaagagc
    18721 gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc gtaagcccac
    18781 cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt accattggtg
    18841 tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc gagcacgtag
    18901 aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc cgccgagatc
    18961 cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg tacaatgtgg ggaatgatgc
    19021 tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt cctggttacc
    19081 tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct atccctttcc
    19141 tgtttggggc tgccgggatg ctggtcaacg gttacgttac tgactggctg gtcaaagggg
    19201 gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc tgttctgccg
    19261 cctttacgct gatagtacca caagcgacaa catccatgac ggcggttctg ctgattggca
    19321 tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc cacgtcgcag
    19381 ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc ttcatctgcg
    19441 cctcttttgc gccgatcatt actggtttta ttgttgatac cacccactca ttccgtctgg
    19501 cactaatcat ctgcggttgc gtcaccgcag cgggggcact ggcgtacatc ttcctggttc
    19561 gtcagccgat caacgaccca cgtaaagatt aatctcctta tgcccgataa cattttgctt
    19621 atcgggcatt ttcatggcga ttaaaatgtt tcccagttct cctcgctatc tgccacggcc
    19681 attttacgcg gcgcagctgg cgtcacggtt tttaccacag ccgatgtttc acgctgctgt
    19741 tgctgctgtt gctgaatccg gaacactgcc acagctttgg tcaggcgact ggcctgctct
    19801 tccagcgcgg cggcggcagc ggcagattct tccaccagcg cggcgttctg ttgagttacc
    19861 cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg ctcatcagaa
    19921 gcagaagcaa tttcgcccat aatgtccgtc acgcgggtca cggcgctgac aatctccgcc
    19981 attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc caccgagtct
    20041 tcaatcaggc ttttaatttc acgagccgcc tgagcgctac gctgggccag attacgcact
    20101 tctcccgcaa ccaccgcaaa accgcgccct tgctcacccg cacgcgctgc ttcaaccgcc
    20161 gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt caattacgct gataatatcg
    20221 gcgattttct gcgaactggt ggagatatcg cgcatagtct gcaccacgtt atctaccact
    20281 ttgccgcccc gttgcgccgt ttcagaagca cttaatgcca gatggctggc ctggcgcgca
    20341 ttctcggcgt tctgtttcac cgttgcggtc agttgctcca tgctggctgc cgtctcttcc
    20401 agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt agcgatttcg
    20461 ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt acgcatcagc
    20521 tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga gccatccacc
    20581 tcaatcggtt tcaccagatc gccgcctgca atatgacgga tgctgtcaat caggcgattc
    20641 attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac cgccagtacg
    20701 acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt gttatcgctg
    20761 acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg cttctcgaaa
    20821 ccgtcttgat atccctgggt cggctgatca aagaactcgt tgattttgcc tgcacctaac
    20881 agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt gatctctgcc
    20941 gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatccgc ccagtttttt
    21001 tccgcctgtt tcagcgaaat actggcactc tgcatcagct cagcaacggt tgaaccgcta
    21061 ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt gttacgcgtc
    21121 tgcaacaacg cgacccagct gccattcagc gtggattgct gctggcgaat ggtttgtaaa
    21181 acagtgaaat tttctttgtc attctttaag gcattaaaga acagaccgcc tgatgtcagt
    21241 tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt gatacgtttt
    21301 aacatggttt ctctttcctg tggaccgatg caagattttc ggcctggaaa gcaaaaactt
    21361 tatgaaaagg tgtaacccgc catccatgga aaaaccaggc gctggttatc agatactaaa
    21421 aagttattca ttatcgcccg ggatggcttc ttgtcgggaa ttgcccagag caataaattc
    21481 ctctaacaga tgggttaact gttgcatttt ctcggcagta aactgagctt caatctgccg
    21541 ataggcttct tcaatctgtg tctgagcgcg gttatacagc gcctgcccct ctttggttag
    21601 cgagatatag agcttccgct gatcattgat cggctttaat cgcaacacta aaccgtcgcg
    21661 ttccatccgc gtgaggatcc cggtcaggct tgggcgcaaa atgcaggcgc gatacgccag
    21721 atcgtgaaaa tccattgatg ggctttccgc cagaatacgc acgatgcgcc actgttgctc
    21781 agtcaaatta tgccgtttaa cgattgggcg aaaataactc atcgccgctt ctcgcgcctg
    21841 gagcaacgca atggttagag agtcgtgcat tatctttccc ctgaaaaatt caaaattatt
    21901 aatatagaaa cagttaatca tcacgttacc gggactgcgc tgcccctgca attcttcatt
    21961 ttatcaaaat tga
//
LOCUS       DAZUFB010000079        21895 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZUFB010000079 DAZUFB010000000
VERSION     DAZUFB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.079"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..471)
                     /locus_tag="ACHY3M_003343"
     CDS             complement(<1..471)
                     /locus_tag="ACHY3M_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS4318195.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAA"
     gene            complement(674..1075)
                     /gene="nikR"
                     /locus_tag="ACHY3M_003344"
     CDS             complement(674..1075)
                     /gene="nikR"
                     /locus_tag="ACHY3M_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFS4318196.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1081..1887)
                     /gene="nikE"
                     /locus_tag="ACHY3M_003345"
     CDS             complement(1081..1887)
                     /gene="nikE"
                     /locus_tag="ACHY3M_003345"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFS4318197.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1884..2648)
                     /gene="nikD"
                     /locus_tag="ACHY3M_003346"
     CDS             complement(1884..2648)
                     /gene="nikD"
                     /locus_tag="ACHY3M_003346"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFS4318198.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2648..3481)
                     /gene="nikC"
                     /locus_tag="ACHY3M_003347"
     CDS             complement(2648..3481)
                     /gene="nikC"
                     /locus_tag="ACHY3M_003347"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFS4318199.1"
                     /translation="MNFFLSSHWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3478..4422)
                     /gene="nikB"
                     /locus_tag="ACHY3M_003348"
     CDS             complement(3478..4422)
                     /gene="nikB"
                     /locus_tag="ACHY3M_003348"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFS4318200.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4422..5996)
                     /gene="nikA"
                     /locus_tag="ACHY3M_003349"
     CDS             complement(4422..5996)
                     /gene="nikA"
                     /locus_tag="ACHY3M_003349"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4318201.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6107..6694)
                     /gene="acpT"
                     /locus_tag="ACHY3M_003350"
     CDS             complement(6107..6694)
                     /gene="acpT"
                     /locus_tag="ACHY3M_003350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFS4318202.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6749..7798)
                     /locus_tag="ACHY3M_003351"
     CDS             complement(6749..7798)
                     /locus_tag="ACHY3M_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4318203.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7930..9147
                     /locus_tag="ACHY3M_003352"
     CDS             7930..9147
                     /locus_tag="ACHY3M_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318204.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9151..9708)
                     /gene="dcrB"
                     /locus_tag="ACHY3M_003353"
     CDS             complement(9151..9708)
                     /gene="dcrB"
                     /locus_tag="ACHY3M_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFS4318205.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9781..10446)
                     /locus_tag="ACHY3M_003354"
     CDS             complement(9781..10446)
                     /locus_tag="ACHY3M_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFS4318206.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10667..10912
                     /gene="tusA"
                     /locus_tag="ACHY3M_003355"
     CDS             10667..10912
                     /gene="tusA"
                     /locus_tag="ACHY3M_003355"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFS4318207.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGRPYRYLIRKGG"
     gene            complement(11014..13212)
                     /gene="zntA"
                     /locus_tag="ACHY3M_003356"
     CDS             complement(11014..13212)
                     /gene="zntA"
                     /locus_tag="ACHY3M_003356"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFS4318208.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13286..13912)
                     /locus_tag="ACHY3M_003357"
     CDS             complement(13286..13912)
                     /locus_tag="ACHY3M_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFS4318209.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14053..14412
                     /locus_tag="ACHY3M_003358"
     CDS             14053..14412
                     /locus_tag="ACHY3M_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFS4318210.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14415..14684)
                     /locus_tag="ACHY3M_003359"
     CDS             complement(14415..14684)
                     /locus_tag="ACHY3M_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFS4318211.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14674..15270)
                     /gene="rsmD"
                     /locus_tag="ACHY3M_003360"
     CDS             complement(14674..15270)
                     /gene="rsmD"
                     /locus_tag="ACHY3M_003360"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFS4318212.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15420..16916
                     /gene="ftsY"
                     /locus_tag="ACHY3M_003361"
     CDS             15420..16916
                     /gene="ftsY"
                     /locus_tag="ACHY3M_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4318213.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16919..17587
                     /gene="ftsE"
                     /locus_tag="ACHY3M_003362"
     CDS             16919..17587
                     /gene="ftsE"
                     /locus_tag="ACHY3M_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4318214.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17580..18638
                     /gene="ftsX"
                     /locus_tag="ACHY3M_003363"
     CDS             17580..18638
                     /gene="ftsX"
                     /locus_tag="ACHY3M_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4318215.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRARITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18883..19737
                     /gene="rpoH"
                     /locus_tag="ACHY3M_003364"
     CDS             18883..19737
                     /gene="rpoH"
                     /locus_tag="ACHY3M_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFS4318216.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20008..21111
                     /gene="livJ"
                     /locus_tag="ACHY3M_003365"
     CDS             20008..21111
                     /gene="livJ"
                     /locus_tag="ACHY3M_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFS4318217.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGMATDAK"
     gene            complement(21310..21693)
                     /gene="panM"
                     /locus_tag="ACHY3M_003366"
     CDS             complement(21310..21693)
                     /gene="panM"
                     /locus_tag="ACHY3M_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFS4318218.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNLGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5203 a         5773 c         6053 g         4866 t
ORIGIN      
        1 tgcggccagc cggtgacctt catccagttt cgccacaccg ccgcgcacca gccagagtga
       61 ttcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc tcaaaataca ggcttctgcc
      121 gccgttatca ctgagtatca gtgtgttatc gcgcagctgt aagcggatat ccgcaggcat
      181 tttccagccg gggccgaggc tccccaccgg cgcgggcgtt tttgtccggt aactgctgta
      241 ggtgcgggag agaatgaacg gcagcgggcc gggcagggcg atgtcggttt caccgggaag
      301 gacctttgca cccagcagcg gattgaccgg atggccggac gtcaccccgc cggggcacac
      361 cgaacaggcc acgccggtgg gggcaccaat acgtaccccg gctgaaccct gaacaatgct
      421 accgccatac tgcgtcatgt caccctgacg cgccgccggt tttccgctca tcgtaaaatc
      481 cttttctatt attttaatga cagtatcttc atacgaaaaa gcgaagaaaa acttgtggtc
      541 acaatgaatt gaagattttt aggaatatat ttagaacgtt acaaaagaga aggttcggat
      601 agggtattta gagaggaatc aggtgtgcta gtggcccttt gttgatcgtt caccaaagga
      661 cccaataata gactcaatct tccttcggca agcactgcaa atgcccgtgc cgcacgccgc
      721 gctgggcgat aacgtcatcg gcaaaatgct gcacgtcgcc catatcacct ttcagcacgg
      781 cgatttccag acagtcgtcg tgattgatat gcacatgcag cgtggcgacg gagagatcgt
      841 ggtgatggtg ctgggtggag acaatgcggc tggctaagtc gcgtttttcg tgttcataca
      901 cataggagag caccgcgaaa ccttgcgtgc cgtgctgctg agtggcctcc tgggccagag
      961 cgctacgcag aatatcgcgg atagcttcgg aacgattgtt ataaccacga cgctggctca
     1021 ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt tgcatttgcg
     1081 ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg ggtaataccg cgttttgtag
     1141 cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accacctggg tttcgacgat
     1201 ttgtccgttg tccatcacca tcacccgctg acagaagcgt tccaccaggc gtaagtcgtg
     1261 ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc tttttcagca ggcgaatgac
     1321 gcccgcctgt aacacgagat caaggttaga aacggcttca tccagaatca gtagtttcgg
     1381 ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc tggccgccgc ttaactgcgg
     1441 tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc ttcagcattt cgctggcgcg
     1501 cgccagttgt tcggattttt tcagtgatag caggtggcgc atcggttcac gcaggatctc
     1561 gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc tgaaatacca tctgaatatc
     1621 gcggcggaac gctttacgct gggcgcgatt gagtttcgcc agcggttcgc cacgccagct
     1681 aatattcccc tgcgcgggcg attctaaacc caccagcaac cgcgcgaggg tacttttccc
     1741 gcagccgctg cgccccagca gggcgacggt ttcgccgctt ttcagggtca gggaaacgtt
     1801 attcagcacc gcctgatgtt gatgttttcc gttaaatcca ccgtgcgcat agtgatggga
     1861 aaggccggag atgttaagta aagtcatgat gccagctcca taccgtagag ggcgagatga
     1921 gcggaaacca ggctgcgcgt cactgtatgt ttgggggcgt taaacagcgt ttctacatcg
     1981 ccctgttcga caatcttacc gtcagacatc accgccacgt catccgccag acgcgccaca
     2041 acgcccatat catgggtcac cagcagcatt cccggcgctt gtttttgcat aatgctttcc
     2101 agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg
     2161 atgataaacg gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg caacatgccg
     2221 ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg cgttttccag ccccaccgct
     2281 tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag gcaggtttcc
     2341 cgcgcgtggg tgtgcatggt gtgcagtgga ttaaaggcgc tgcgcgggtt ctgcatgatg
     2401 gtggcaattt tgatgccgcg cagggcgcaa ggcgaaaccg gtttgccatc ggctaaaatt
     2461 tcccccgccg tctggcgaac gccagcgggc aaaatgccca gcgtcgcggc gcaggttaat
     2521 gatttcccgc tgccgctacc gccgactaac gccagcacgc gcccgcgttg cagggttaac
     2581 gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgcga tattacgtag ttcaatctgt
     2641 tgcggcatta gtgtgcgtgc tccgtcacca gatgagggtc cagatgatcg cgcagtgcgt
     2701 cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt
     2761 gcagcggctg ggtccagata tactggcgcg cgtcgttaat catcacgccc cattcggcgg
     2821 tcggcgcggt cacaccgagg ccaaggaaag acatccccgc gacgtgcagc atcatatggc
     2881 cgatatccag cgttgccagc accagcagcg aagggatcac cgcgcctgcc agatgatcga
     2941 caaacacccg cacatggccc gcaccggaaa gccgtgacgc cagcacaaac tcgcgttggc
     3001 gtagtgaaat caccaggctg cgcaccatgc gtgcatacca cgcccagtgc gacagggcga
     3061 tggcgataat tacgttggtc agcccggtgc cgagcacgcc aaccataaag aacgacagaa
     3121 tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat ggtggcctga tcaacgcgcc
     3181 cgccaatcaa cccggcgctg ccgccaataa ccagccctaa tgtcagcacc agcagcaggc
     3241 aggccattac cgaaccgagc gacacgcggg tcgctgtcat cagccgcgag aaaatatcgc
     3301 gacctaagtg atcggtgccc agccagtgct gcgcatccgg cgaaagcagg cgcgacggca
     3361 aatcaatcgc ctgggggtca tacggcagcc accactggct ggtgagcgca atcagcgcca
     3421 gcagggcgat aatgatcagc gccaggcgta ccgaccagtg ggaagagagg aaaaagttca
     3481 cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc gcggcgttga gcaaatcgac
     3541 aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact ggatcaccgg
     3601 atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc aggcaaagat
     3661 gttttcgata atcatcgtcc cgccaatcag ttcgccgata tgcatcccca cggcggtgat
     3721 catcggcagc gaggcattgc gcaggatgtg gcgacgttcg gtctgtttgt cgttcaggcc
     3781 gcgcagacgc gcccaggtga cgtgacgctg accggcgacg tccagcatac tggcgcgcag
     3841 taaacgcgcg ttaatcgcca gcgacataaa ggcaatggaa accgcaggca aaatgatgtg
     3901 ctgccagccg ccgtaaccca tcgcgggtag ccattgcaga tacaccgaaa acgccatcac
     3961 cagtaaaaac gccagccaga agttaggcat cgacacgcca agaaacgcga tgaaacgtac
     4021 ggcgaaatcc ggcagacggt cgcgatggcg cgccgcccag ataccgagcg gtacggaagt
     4081 gagcagaatt aataccagcg ccgcacctgc aagttccagc gtggcgggca ggaagttcag
     4141 catatcgtcc agtaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc
     4201 cttccacaac caggtaccgt actggacgta cagcggctga tccagcccga gcatggtgcg
     4261 ggtagaggcc agcatctccg gcgtcggcgg caggttagat aaacgcagat aatcgagcgc
     4321 cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg cgagcaccat
     4381 cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt caccggttta
     4441 atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatgttacc cagctccggt
     4501 tttgatacca ccatcattga gatgtaactg ataggcagat aaaccgcctc gtcatgcaga
     4561 cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt cgccagcact
     4621 tcgccgatct ctttatcaat cagcggtttg tcggctaatc cttgctgtgc ctggaagtca
     4681 gcgtgtgacg gtacgcgcat tgaactgagg aaggcgtgtg gatcatatgg cgcgccccag
     4741 gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct
     4801 tcttcgccaa tcagcgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc
     4861 gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga
     4921 ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc aaccggcttt ttccagcaac
     4981 gctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgaggttggc gtagggcaca
     5041 gaaggggcaa acagggtgtc ggcgacctgc tgggtgccat acaacgcgtt atcaatcagc
     5101 gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct
     5161 ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg ggtgtggtaa
     5221 gccggattct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac
     5281 agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatgacg
     5341 ttaaaggtga tctttttaat cgctggcttt tcgccccagt agttttcgtt acggacgaag
     5401 acatcgtact gattcagttt cgattcctgc aaaatccacg gtccggtgcc aatcggcgct
     5461 ttaattccgt tcatggtttc atggttttta aactgcgagg gagcgataaa acggaaagga
     5521 cggggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt aatttgcagc
     5581 tctgttttac tgagtgcttt aacatcaaca atctggtttg ccagctccag ccaggcgtga
     5641 cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc atcgaacggt
     5701 tcaccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt accatcttct
     5761 gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
     5821 aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt
     5881 agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg
     5941 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga gagcatggta
     6001 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata
     6061 ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag ttaactgaat
     6121 cgatccattg cacactgtcg gcggtgagcg taaagggagt aggggtgcag atcgccaggc
     6181 ttaaattttc aagctgacaa tggctgactg acagcgagga gtgataggtg ctgtctacgc
     6241 tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga
     6301 tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatctca gcgtgttccc
     6361 cgaggctgaa tacggcgttc gccagccagt gccagttggc gcgcgggcga atcacttcga
     6421 tatcgcagcc gacttcgcct tcatcactca acagcagggc gatatcgtca ccgctatggc
     6481 ttaagttgaa ccatagcggc atttccggcg caaatgcagg tttgccttgt tcgccataga
     6541 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt
     6601 cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagtgga gctgcgctta
     6661 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
     6721 gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg
     6781 gccccagtaa aatcgccagt ttgctaccgc ctttggtggt ttccatccag attttacaca
     6841 cactggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta
     6901 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggtt
     6961 ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc agaatacatt
     7021 cgtaaacacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcgc
     7081 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgca
     7141 cccccatcag ctccagcccc agccagacga tgacacctgt ccataaactg agtagcgtct
     7201 tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg taatcctgcg atgtgaatct
     7261 gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa
     7321 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
     7381 gcgctgtggt gaaggtaacc actttttccg agtccatccg ctgcagcatt cgctccggcg
     7441 acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcaact
     7501 ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga
     7561 ccagcgcagt tagtgcgatc agcatcacca ccaccacaat cgtaatggca atggggcgtt
     7621 gtactccccg acgaataaac caggtgacga gcgggttgag gacgatggca aaaaacagcg
     7681 ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
     7741 gcgaggccag cttgagcaga atgtgcatgc ccgttttatc gggttgaggg gtttccattg
     7801 gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca atattctgat
     7861 tcttactgct gattttcacg tcagcacgtg gtaaaaatga aacactgttg taaaaatgtg
     7921 gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg cctgaatttg
     7981 cgcattgtct ccattgtcat gtttaacttc gccagctacc tcaccatcgg gttgccgctc
     8041 gctgtattac cgggctatgt ccatgatgtg atgggcttta gcgctttctg ggcaggattg
     8101 gttatcagcc tgcaatattt cgccaccttg ctgagccgtc ctcatgccgg acgttacgcc
     8161 gatttgctgg gccccaaaaa gattgtcgtc ttcggtttat gcggctgctt tttgagcggt
     8221 ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactt
     8281 tgcctgggac gcgtgatcct tgggattggg caaagttttg ccggaacggg atcgaccctg
     8341 tggggcgttg gcgtggttgg ctcgctgcat atcgggcgag tgatttcgtg gaacggcatt
     8401 gtcacttacg gggcgatggc gatgggtgcg ccgttaggcg tcgtgtttta tcactggggc
     8461 ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat tttgttggcg
     8521 atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg cgcggtgctt
     8581 gggcgcgtct ggctgtacgg tatggcgctg gcactggctt ccgccggatt tggcgtcatc
     8641 gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg
     8701 acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt
     8761 atcggtggct taaacgtagc gatgatttgc tttagcgttg agataatcgg cctgctactg
     8821 gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg tcttactggc gggggccggg
     8881 ttttcgctgg tgttcccggc attgggtgta gtggcggtaa aagcggttcc gcagcaaaat
     8941 cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgactgga
     9001 ccactggctg ggctggtgat gagctgggcg ggcgtaccgg tgatttatct ggcggcggcg
     9061 ggactggtca caatcgcgtt attactgacg tggcgattaa aaaaacggcc tccggaacac
     9121 gtccctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt
     9181 ttctgcggtg gtctgcgctt tttgctgatc gtcagctggc agagtaattt gcatggtcag
     9241 cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc ctttcgcgga
     9301 gataatactg tctaactgct gcattttgtg acctttcagc tcaatggctt tattggttac
     9361 cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg ccagcaccgc
     9421 cagatcttct tttggatcat cgcccatgat gacgatgact gctttctgcc cggtggcgtc
     9481 ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt cggtcatatc
     9541 cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc cggtagcgtt
     9601 actttccgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac aggccgcaag
     9661 ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg acttcttcct
     9721 ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga taacgaacct
     9781 ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca acagcatatt
     9841 gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt tgaaacagta
     9901 atcgaccagc gcgatttcca tccagtgttc agccataaag gcatccgggc tacgccagaa
     9961 ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg
    10021 tgccagccac cagcggcgac tttgacgcag gcggttaaaa acgtgcacgt cgaggatttg
    10081 ccccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg
    10141 ggcgagtgcg ccgaatccct gccatgaacc catatagaac agcgacgaga tgacatagga
    10201 gattaataac gcagggatca ttaccgcgaa gataatgcgt cgggccagcg gtgcgccaaa
    10261 aatacgcacg gtcaggtcgg tagcaagaaa aataaacgga aagctaaacg cgccccaggt
    10321 ggtatggaaa ccaaaaatgg agacgggaag ctgaaccaga tagttactgg aggtgatcac
    10381 cagcagatga aataacgata accagaacaa cgccttatag cgttgagttt gcgagaaaac
    10441 gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac gtacgacacg
    10501 tctgttacct taacataata acgtgcttac ccttttttcg agccgccgca tgatactgct
    10561 ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt aacctggttt gcttacggac
    10621 tcacagggcg taaactagcg ccgttttttt atgtgatgag aagaaaatga ccgatctctt
    10681 ttccagccct gaccacacac tcgacgcact tggcctgcgc tgcccggaac ccgtaatgat
    10741 ggtgcgcaaa actgtgcgca atatgcagcc cggcgaaacg ttgctgatta tcgccgacga
    10801 tccggccact acccgcgata ttcctgggtt ttgtaccttt atggaacacg aactggttgc
    10861 gaaagagacg gatggacggc cttatcgtta tttgattcgt aaaggcggtt gatgggggct
    10921 gattggcttc gatgccgcct tttcccctca ccctaaccct ctccccagag gggcgagggg
    10981 accgatcgcg ctcaatgttg cgatcggttt gccttatctc ctgcgcaaca atcttaacgc
    11041 attcgctgtc accagcaccg tcgcacccgt atctgccagc accgccagcc atagtccggt
    11101 catccctaac agcgtggtga cgaggaagat ccctttcagt cccagcgcaa tggtgatgtt
    11161 ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca ggccgcgcag
    11221 gtggttatgg gttaatgctg catcggcggt ttccagcgcc acgtctgtgc cgctgcccat
    11281 tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg ttaataccat caccgaccat
    11341 cgccagcggc gcatgttgat tcagcttggt caccgctttg actttctctt ccggcaatag
    11401 gcccgcttta aactccagcc ccagctcccc ggcgattgcc gccgctgcgc gtggattatc
    11461 gccggtgagg atcactcctt taactcccag cgcgttcagt tcactgatgg cagttgcagc
    11521 gtcggcgcgc agggtatcct gcaatgcaat gatacccagc acgtcatcat tacgtactac
    11581 cagcacaacc gtttgcccgg cgctttccag ttcattaatc aaaccagcaa atgcatcagc
    11641 gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg ttaacctgcg cttcaatgcc
    11701 agatccgacc agcgcccgct gtgattcggc ggtgggaatg gcgagttcag caacctgtgc
    11761 ttctcgcacg atggcctgcg ccagtggatg cgtcgcacct tgctcgaccg ccgctgccag
    11821 tgtcagcagt tcagattcac taatacccgt tgccggatga atcgcggtaa cgcgcggttt
    11881 accgacggtc agcgtaccgg ttttatcaaa cgccacctgg gtaacacgac ctagttgttc
    11941 cagcgccgct ccgcctttaa ttaacgcccc acgacgcgct gctgccgcca gcccggaggt
    12001 aatcgccgcg ggcgtggaga taactaacgc acacgggcag ccaatcagca gcagcgtcag
    12061 gcctttataa atccactctt gccagctggc ggcaaacagc agcggtggca ccagcgtcac
    12121 cagcagagcg acggccataa tcgcgggcgt atagatacgg ctgaaacggt cgataaaccg
    12181 ctcaatgggt gcgcgacgct cttcggcttc ttcgatcagt ttcagaatcc ggtcaatggc
    12241 gctggcaccc ggttctgaca gcacttccag cgttaccaga cggtctacgc tggtggctcc
    12301 agcagggact ttatcgcccg ttgcgcgctc caccggaata gattcgccgg tcagggcgct
    12361 ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg gcaggcaaac gtccacctgc
    12421 ggcgacttca atcacatcgc cagggcgcag gctgttaatc gccacctctt cccgctcacc
    12481 gttacgcagg cgcgtggcgg tttctggttt cagcgccatt aacgcgctga ccccttgacg
    12541 tgcgcggctg gcggcccagc cttccagtcg ttcgccaatc aaaaacagca gcaacaccat
    12601 cgcagcttca gccgttgcgc caataaaaag cgcaccaata gcggctacgc tcattaaggt
    12661 ttcaatggcg aagtagctgc cggatttgat caaccgtaat gcctgacgag caatcgggta
    12721 cagcccaacc agtgtggtcg cgataaacgc cagttgcccg aacggatgat tgaactgctc
    12781 cagaccccag ctgattgcca tcatcacgat tagcgtaatc agcggcagat tctctttcag
    12841 gcgtgatgct tgcggttcgt cggcggactg ttcatcgcgc agggagtagc ccgctttttg
    12901 caccgcagat tcaacctggg cacggatgtc attgtcggca tcaaccacca gtttttcggt
    12961 ggcgaacaac acctgtacct gattcacgcc tgcaagctgg cgcacggcat tttctacctt
    13021 gcgcgcacag gcggcgcagt ccatgccgct gactttccag ctatagcggg tgccggagac
    13081 gttttcagag agagttggcg tgctggaaca tgcgccgtcg cagcaacagt cgttggcgtt
    13141 ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg gctttcttgc cgtgattgtc
    13201 aggagtcgac atggcatcct ccggttaagt tttttctcat taaccgaagg atacactctg
    13261 gagtcgactc cagagtcaag ttttatcaga gatacagcga gcggacgatc aggaagtgac
    13321 cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg cgatagtgac
    13381 tacccagcca gacaaagtta ctgataaaca gcaacgaagc gccgacaaac gcagagagcg
    13441 ccggagcggt cggacggaag aaccacagtt cacctgccag ccacaccatc accagcgtca
    13501 tgccgataaa cgtgcagata ggccaacgat actcttccag ccgcgtccag ataatcgcca
    13561 gtaacagcgc gcccagaacc agcagcacca gtggcagagg ccagaagaaa gagagcgtca
    13621 tctgactggc gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt
    13681 acatcagacg ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag gcgcacagcc
    13741 ccgccagcac cagatagcta atggcgtcga acatcggcgc ttgccaggcc agtaacagca
    13801 ggagaagaag ggttaatggt ttaaataccc agcgttgcca ggttggccca cgatacgatg
    13861 catccacaga tagccatgcg gaaagacaga cagcgataaa cgaccaaagc atcttagttc
    13921 cttgaattcg ttattttttc gtaagcggaa tgcctacggt cttgcttcca gtttagtggc
    13981 gagggtggac ggatgacaac accataactg gcagggtatg cttaattccg cattttccga
    14041 tgagggataa agatgagcaa accacctctt ttctttattg ttatcattgg cttaatcatc
    14101 gtcgccgcat cgtttcgttt tatgcaacaa cgacgggaaa aagctgataa tgatatggca
    14161 ccactccagc aaaagctggt gttggtgagc aacaagcggg aaaaaccgat taacgatcgc
    14221 cgttcgcgcc agcaggaagt gactccggca ggcaccagta tgcgctatga ggcaagcttc
    14281 aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg acgcccagca gtaccacgcc
    14341 ctgacagtgg gcgataaagg tacgctgagc tataaaggaa cgcgctttgt cagctttgta
    14401 ggcgaacaat aaggttattt tttaacttta aatttcttct gccagaccag cagttcaaac
    14461 acgccgaaaa ggaaaatccg taccttttcg gcggtggtca tctgcgggcc atctttcggt
    14521 aaagtggatt tcaatagcgc cagctgcata ccatgcatca gcacggtaaa aatcagcgcc
    14581 acgttaacga aaatattcag cggacgtggg aagggatgca ccaggttgag gattaaaaat
    14641 ccccaaacac agagcattaa caaacgacca atattaatca gcatcacttt ctccttgtgc
    14701 ttcgcgttga tacagccgat aagccacctg acccgccact ttttcccgat gtaatgacca
    14761 gtttgccgga acagtgggca gaccgttttc gacttcgctt tcgacataaa tcagggcttc
    14821 gtcagccagc cagccgttat cttccagtaa atttatcgtc tcttctaaca agccacggcg
    14881 gaacggtgga tcgacaaaca cgatattatg cggtgtacct ttttgcgcca ggaatgacat
    14941 cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat tcttaattaa
    15001 ctgctgagaa accgcgcgat ccatctcaat caacgttgcc cccgcagcat agcgcgacaa
    15061 cgcttccagc cccagcgcgc cgcttccggc gaagcaatcc agacattggg cgtcaacaat
    15121 gaccggagcc agccagttaa acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc
    15181 tgggctatca ggaaccggga gtttacggcc tcgccactgc ccgccaataa tgcggatttg
    15241 gccgctgccg gaatgattcg gttttttcat gataaattgc tcaatccgcc agataacaca
    15301 tacttgcagg cggtgatgtg aattaagtta agtgatagac tatttcatca tttttttagc
    15361 tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa
    15421 tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa aaagagcaga
    15481 ccccggaaaa agagacagaa gttcagaatg aacaaccggt tgtagaagaa atcgttcagg
    15541 cgcaagagcc tgtgaaggct tcggaacaag ccgttgaaga gcagccgcag gcgcatactg
    15601 aagccgaggc ggaaactttt gctgccgacg ttgtggaagt cactgaacag gttgctgaaa
    15661 gtgaaaaagc gcagcctgaa gcggaagtcg ttgcacagcc ggaaccggtc gcagaagaaa
    15721 cgccggagcc agtggctatc gaacgtgaag agctgccgtt gccagaagac gtcaacgccg
    15781 aagaggtttc gccagaagag tggcaggctg aagcggaaac cgtagagatt gtcgaagcgg
    15841 cagaagaaga agcggctaaa gaagaaatca ccgacgaaga gctggaagca caggcgctgg
    15901 ctgccgaagc ggcagaagag gcggtgatgg tagttcctcc ggcagaagaa gagcagccgg
    15961 tggaagaaat tgcccaggag caagaaaaac cgaccaaaga aggtttcttc gcgcgcctga
    16021 aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc ctgttccgcg
    16081 gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga acagctgttg atcgccgatg
    16141 tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca tcccgcaagc
    16201 agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggc gagattctgg
    16261 cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg
    16321 gcgtcaacgg tgtgggtaaa actacgacga ttggtaagct ggcacgtcag tttgagcagc
    16381 agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg gttgaacagc
    16441 ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc ggtgcggatt
    16501 ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc gacgtcctga
    16561 ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag ttgaagaaaa
    16621 tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg ctgactattg
    16681 atgccagtac cgggcagaat gcggtaagcc aggccaaact gttccacgaa gccgttggct
    16741 taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta attttctcgg
    16801 tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt attgaggatt
    16861 tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag gattaacaat
    16921 gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc tgcagggcgt
    16981 tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg gcgcagggaa
    17041 aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga aaatctggtt
    17101 tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat
    17161 tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg ataacgtggc
    17221 gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg tgtcggcggc
    17281 actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc tttccggcgg
    17341 tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg tactgctggc
    17401 ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac gtctgtttga
    17461 agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca acctgatttc
    17521 acggcgttcc tatcgcatgc tcaccctgag cgatggtcac ttgcatggag gcgtgggcca
    17581 tgaataagcg cgatgcaatc aatcatattc ggcagtttgg cgggcgtctt gatcgcttcc
    17641 gtaaatcggt cggcggctct ggcgacggcg gtcgtaacgc cccgaaacgc gcgaaatcct
    17701 cgccaaaacc ggtaaatcgt aaaaccaacg ttttcaacga acaggtgcgc tatgccttcc
    17761 acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg gtgatggtta
    17821 tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac gttaatcagg
    17881 cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa acgctggacg
    17941 atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg gagaaagtga
    18001 actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt tttggtggtg
    18061 cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg
    18121 attttcaggg gacggaatca ctcaatacgc tgcgtgctcg tatcacgcag attaacggca
    18181 ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg accgggctgg
    18241 tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc ctcgtcatcg
    18301 gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg cagaaactga
    18361 ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca ctgctgggat
    18421 tttctggcgc attgttgtca ttaattttgt cagaaattct gatgctgcga ttgtcatcgg
    18481 cggttgcgga agtggcacag gttttcggaa cgaagtttga tatcaatggt ttatcgttcg
    18541 atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca gcgtggcttg
    18601 ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt atactctttc
    18661 cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg tatttcatct
    18721 ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt ggataaaatc
    18781 acggtctgat aaaacagtga atgataacct cgttgctctt aagctctggc acagttgttg
    18841 ctaccactga agcgccagaa gatatcgatt gagaggattt gaatgactga caaaatgcaa
    18901 agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc taacgcgtgg
    18961 ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta ccatggcgat
    19021 ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca tattgctcgt
    19081 aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa catcggcctg
    19141 atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc cttcgccgtt
    19201 cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat cgtcaaagtt
    19261 gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaaccaa gcagcgtctg
    19321 ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt aaccagcaaa
    19381 gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga cctgtcttcc
    19441 gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga taaatcatct
    19501 aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa ccgtctgacc
    19561 gacgcgatgc agggtctgga cgaacgcagc caggacatca tccgtgcgcg ctggctggac
    19621 gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc cgctgaacgt
    19681 gtgcgccaac tggaaaagaa cgcgatgaaa aaacttcgcg ccgctattga agcgtaattt
    19741 ccgctattaa gcagagaacc ctggatgaga gtccggggtt tttgtttttt gggcctctac
    19801 aataatcaat tccccctccg gcaaaacgtc aatccccacg cagattgtta ataaactgtc
    19861 aaaatagcta ttccaatatc ataaaaatgg ggtatgtttt agcagagtat gctgctaaag
    19921 cacgggtagt catgcataaa acgaaataaa gtgctgaatc acaacaccac aacacacgta
    19981 ataaccagaa gaatggggat tctcaggatg aatataaagg gtaaagcgtt actggcagga
    20041 tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt cgcggtcgtg
    20101 ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg cgcagagcag
    20161 gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca aatcgtaaaa
    20221 tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac
    20281 ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc gtctgatatc
    20341 tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga gctgaccgcc
    20401 cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg gccgacggcg
    20461 gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca cgacaaacag
    20521 caatacggcg aaggtctggc acgagcggtg caggacggcc tgaagaaagg caatgctaac
    20581 gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctctacgct ggtggcgcgt
    20641 ctgaagaaag agaatatcga cttcgtttac tacggcggtt accacccgga aatggggcaa
    20701 atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc ggaaggtgtg
    20761 gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag
    20821 ccgaagaact acgatcaggt tccggcaaac aaacccattg ttgacgcgat caaagcgaaa
    20881 aaacaggacc caagcggcgc attcgtttgg accacctacg ccgcgctgca atctttgcag
    20941 gcgggcctga atcagtctga cgatccggct gagatcgcca aatacctgaa agcgaactcc
    21001 gtggataccg taatgggacc gctgacgtgg gatgagaaag gcgatctgaa aggctttgag
    21061 ttcggcgtat ttgactggca cgccaacggc atggcgaccg atgcgaagta atcattaatc
    21121 ggcaactttg ggttgccgcc aaattgctaa tatcgagtac gttgcttcat gccggatgcg
    21181 gcgtaaacgc cttatccggc ctacaagatc caaagaaatc agtaaattgc aacacacatt
    21241 gtaggcctga taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca
    21301 acttgacgat taacgcttct cccagccgcc ctgttgcgcc gtaaacccca gcgcctgcat
    21361 aaacgccgtc atcacaccgc gatcttccaa gcctgcatcc gccatccacc agcatgaaac
    21421 gccaagattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacgtcgggt
    21481 gacttcccgc acacgcaggg aatccagtgc tccctcggtg ccgcttaagg ttacccgtac
    21541 ggcagcgagc aggcgctcgt taaaacgcgc ggcgtagata cggtggttat cgtcaacctg
    21601 taaagaggaa ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct
    21661 aaagttttct aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg
    21721 gaaccagtgt agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg
    21781 tgattaattt tttaacagca ataattacaa agttaaaaca ttagagaatg aaaaataccc
    21841 agcataatcc cctgaatggt agtgaattat tccgcccttt gtgccgttat tttat
//
LOCUS       DAZUFB010000080        21867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZUFB010000080 DAZUFB010000000
VERSION     DAZUFB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.080"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1627)
                     /locus_tag="ACHY3M_003367"
     CDS             complement(<1..1627)
                     /locus_tag="ACHY3M_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFS4318219.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALAGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGS"
     gene            complement(1738..3150)
                     /gene="ftsP"
                     /locus_tag="ACHY3M_003368"
     CDS             complement(1738..3150)
                     /gene="ftsP"
                     /locus_tag="ACHY3M_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFS4318220.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3225..3962)
                     /gene="plsC"
                     /locus_tag="ACHY3M_003369"
                     /gene_synonym="parF"
     CDS             complement(3225..3962)
                     /gene="plsC"
                     /locus_tag="ACHY3M_003369"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFS4318221.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4195..6453)
                     /gene="parC"
                     /locus_tag="ACHY3M_003370"
     CDS             complement(4195..6453)
                     /gene="parC"
                     /locus_tag="ACHY3M_003370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4318222.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6999..7481)
                     /locus_tag="ACHY3M_003371"
     CDS             complement(6999..7481)
                     /locus_tag="ACHY3M_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4318223.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(7534..7926)
                     /gene="ygiW"
                     /locus_tag="ACHY3M_003372"
     CDS             complement(7534..7926)
                     /gene="ygiW"
                     /locus_tag="ACHY3M_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFS4318224.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8078..8737
                     /gene="qseB"
                     /locus_tag="ACHY3M_003373"
     CDS             8078..8737
                     /gene="qseB"
                     /locus_tag="ACHY3M_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFS4318225.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            8734..10083
                     /gene="qseC"
                     /locus_tag="ACHY3M_003374"
     CDS             8734..10083
                     /gene="qseC"
                     /locus_tag="ACHY3M_003374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFS4318226.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(10129..10461)
                     /locus_tag="ACHY3M_003375"
     CDS             complement(10129..10461)
                     /locus_tag="ACHY3M_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFS4318227.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            10780..11361
                     /gene="mdaB"
                     /locus_tag="ACHY3M_003376"
     CDS             10780..11361
                     /gene="mdaB"
                     /locus_tag="ACHY3M_003376"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFS4318228.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11392..11706
                     /locus_tag="ACHY3M_003377"
     CDS             11392..11706
                     /locus_tag="ACHY3M_003377"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFS4318229.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11754..13646)
                     /gene="parE"
                     /locus_tag="ACHY3M_003378"
     CDS             complement(11754..13646)
                     /gene="parE"
                     /locus_tag="ACHY3M_003378"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4318230.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13675..14256)
                     /gene="yqiA"
                     /locus_tag="ACHY3M_003379"
     CDS             complement(13675..14256)
                     /gene="yqiA"
                     /locus_tag="ACHY3M_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFS4318231.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNSAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14256..15083)
                     /gene="cpdA"
                     /locus_tag="ACHY3M_003380"
     CDS             complement(14256..15083)
                     /gene="cpdA"
                     /locus_tag="ACHY3M_003380"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFS4318232.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15108..15530)
                     /locus_tag="ACHY3M_003381"
     CDS             complement(15108..15530)
                     /locus_tag="ACHY3M_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFS4318233.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15531..16160)
                     /gene="nudF"
                     /locus_tag="ACHY3M_003382"
     CDS             complement(15531..16160)
                     /gene="nudF"
                     /locus_tag="ACHY3M_003382"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFS4318234.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16365..17846
                     /gene="tolC"
                     /locus_tag="ACHY3M_003383"
     CDS             16365..17846
                     /gene="tolC"
                     /locus_tag="ACHY3M_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFS4318235.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            17994..18665
                     /locus_tag="ACHY3M_003384"
     CDS             17994..18665
                     /locus_tag="ACHY3M_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFS4318236.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18671..19831
                     /locus_tag="ACHY3M_003385"
     CDS             18671..19831
                     /locus_tag="ACHY3M_003385"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS4318237.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19869..20657)
                     /gene="ygiD"
                     /locus_tag="ACHY3M_003386"
     CDS             complement(19869..20657)
                     /gene="ygiD"
                     /locus_tag="ACHY3M_003386"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFS4318238.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20800..21573
                     /gene="zupT"
                     /locus_tag="ACHY3M_003387"
     CDS             20800..21573
                     /gene="zupT"
                     /locus_tag="ACHY3M_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFS4318239.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21631..21801)
                     /gene="yqiD"
                     /locus_tag="ACHY3M_003388"
     CDS             complement(21631..21801)
                     /gene="yqiD"
                     /locus_tag="ACHY3M_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFS4318240.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         5368 a         5880 c         5499 g         5120 t
ORIGIN      
        1 agctgcccat gcccggcttc atcatcttcg ataacggccc ttcttcggta tgttccgggg
       61 caatcttcag ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt
      121 ccacggcgat gtcataacgt acgcctgagg cgatgaggat ttttttaatg cctttcagat
      181 cacgcgcacg gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa
      241 tatccggata aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc
      301 gcaacatata catgttggca gttggcccac cgagatcgga aattacgccc gtaaaaccag
      361 gaacggtgtc gcggatcgct tcgatctcat taatgatcga atcttccgaa cggctctgaa
      421 taatgcgccc ttcgtgctcg gtgatagaac agaaagagca gccgccaaag cagccgcgca
      481 taatgttgac cgagaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg
      541 gatgcggcac gcgcttgtac ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg
      601 ggatagcagg cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg
      661 cgcagcctgg gttggtttcg tggtgcagaa tgcgcgaagc atgagcgtac aggactttat
      721 cgcccttcac tttctcgaaa gaaggcagca atacgtaggt tttttcccac ggtttcgggc
      781 gcggcggctg cacggttaca gctttcgctt cctgcttttt cggcgcaaca ggtttgttat
      841 ccgcgcacgg cagatcttcg ccatacggat gcgggattgg gtcgattttc cctggggtat
      901 caagacgagt agaatccaca ccgctccagc cagccagcgc ctcttttacg ataattgcgg
      961 tattacgcac atcgcggatt tcgctaattg gctcgcccat cgccagacga tgcgctactt
     1021 ccaccagcgg acgttcaccg ttaccaaaca tcagcatgtc ggctttcgaa tccaccagca
     1081 cggaacggcg cacggtatcg gaccagtaat cataatgcgc ggtacggcgc agactagcct
     1141 caataccgcc aaggatcacc ggcacatctt tccacgcctc tttacaacgc tgggtataaa
     1201 ccagtgtggc gcgatccggt cgcttacccg cgacgttatc cggtgtgtag gcatcgtcat
     1261 gacgtaaacg gcgatcggcg gtatagcggt tgatcatcga atccatgttg ccagcagtaa
     1321 caccaaaaaa cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtccg
     1381 gctgggcgat gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca
     1441 tcccgaagct tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat
     1501 cccagccaag ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac
     1561 aggcggccca gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag
     1621 agctcatttt gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg
     1681 aacgaaagag aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta
     1741 cggtaccgga ttgaccaaca gttgcccaat cgagccacgg tccgccattt ccagcgtctg
     1801 actgttgaag tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc
     1861 cacttgtccg tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc
     1921 gccattcacg ttacggatct ggaacatcac gccttcaata tggaacgctt gcggctcgtc
     1981 cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac
     2041 gtcccacagc tgtccattaa tacccgggtc atcacccaga ctaatatcgc gactgcgaat
     2101 cggcgaaccg gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag
     2161 cggcagaagg ccggttgggc gcagcgttag caccagggta gaaaccaaaa tgctggatgg
     2221 ctcaaagaag ccacgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac
     2281 ttcatcaccg ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag
     2341 ttgcttcacc gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg
     2401 gcgaccatcg ctcatctgta gttgatagcg acgagagttc gacgcgttca gcagtcgcaa
     2461 gcgcacccag ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt
     2521 atcaccaaca aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag
     2581 ccgtttatcc tggataatga ccggaaaatc atccacacca taatggttgg ggataggcag
     2641 cgacttgctg acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg
     2701 agccgtgcgg ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatggg
     2761 cagtacgggt gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg
     2821 gcctggtacc tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc ggttgctgta
     2881 aataagctta acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt
     2941 gattccccag accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt
     3001 cataaacagc ggttgcccac ggcgagattc aagtagcggc ggaacgggta gcggttgctg
     3061 ttgcccggct gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc
     3121 ctgaatgaac tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa
     3181 taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg
     3241 gcttcgcgtt ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga
     3301 caatgggcag ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc
     3361 agcatttcga caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt
     3421 gtagagacgc acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca
     3481 gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg
     3541 gaaatacggc gttttttgaa atgattcacg acttccgcaa tggtgccgtg agctttagtg
     3601 cgattgtttc tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg
     3661 atccacagca agctcttttt acctaccgtc accgtcggcg gttgcacaat gttcgatgct
     3721 gtcaccatgt catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca
     3781 gcatctgcag gtttgcggca ctcaactttc aggccaaaca gcggcgcaag acggccaaac
     3841 atatgcccaa aggtggccac atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag
     3901 ccgaatacac agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc
     3961 ataacaccct ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg
     4021 cattgttaaa agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa
     4081 cggtttcaag ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta
     4141 acgtttcatc cggcgttcct tgcaagcggg aggaaacagg ccctccccgg catattactc
     4201 ttcgctatca ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg
     4261 caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtaactcttc
     4321 cgggcgcagt ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag
     4381 aacgtacaat tgcgccagac catcttctcc acgcgcggct tctgccgatg gaatgttgat
     4441 aatcttgttg cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat
     4501 acggcctgcc tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg
     4561 cataacatgg gcattttccg gtaaggtgat caaagcctta cctgcacggt tacgcgccac
     4621 cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg
     4681 atcgtcgctt tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc
     4741 ggtgagtggt tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct
     4801 acgaccggtg gaatcaacaa acactaccgg ttggttgctc ttacctttca ccgccgcttt
     4861 gaagctatca cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct
     4921 gcgtacccag cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg
     4981 ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta
     5041 ggcttgcgcg tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc
     5101 caaaatgccc tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt
     5161 catctcttcc agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt
     5221 ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat
     5281 ctcaatcact tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg
     5341 cttgaggact ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac
     5401 cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat
     5461 catattgagg ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat
     5521 cacctgatcc atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc
     5581 gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc
     5641 agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac
     5701 cgcgccatct tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta
     5761 gattttacgg atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc
     5821 ctgcacgata tccagcagct gatcgagcgt ggttttcggc tggtcgatta atgcgattgc
     5881 cgcctgagct acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat
     5941 accggtggtg ccgttaagca aaatgtttgg cagacgagca ggcagcattt tcggctcctg
     6001 caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag
     6061 cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg
     6121 atcgtccggc gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg
     6181 ctgcgccatc aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc
     6241 cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag
     6301 ttcagacatc gcatacacaa tgcggcgctg aacaggttta agaccatcgc caataaacgg
     6361 caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc
     6421 atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc
     6481 agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg
     6541 agatcagcgt gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc
     6601 ttgatataca actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc
     6661 catgccacac cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc
     6721 gagtccaggg aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt
     6781 ttagctgtgt ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat
     6841 acccccgaga cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt
     6901 accggagaaa ttataagccc aacaacaata cgcctgtggc attgttcgct caaacttatc
     6961 gcgagtgatt tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca
     7021 tccgtttcca gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacataatc
     7081 ggtgctttac gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc
     7141 gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta
     7201 agttcaccat tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag
     7261 atatcaaagc gaaacgcttc cggtgcggtg gtggcagggt cgtcccaggc gacgccaaaa
     7321 gtctggcttt ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc
     7381 acactgtagt tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat
     7441 gggaaatcga taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt
     7501 attcccggga acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt
     7561 gacgtcaatt tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc
     7621 tttcggcgtc accgtcacac cgttccagcg tttgtggtcg atatcaacat tgatagtacc
     7681 gctggcatct ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag
     7741 ggttacccag gtgtcgtcac gcagggattt tgcgctttct acggtcgtta cgctgccgtt
     7801 cggcccctgg aatcctccgg cctgactttg cgttgccgat gggccagaaa aaccgccctg
     7861 ctctgctgcc atcaccggtg cgctgcacag ggccattact gcgattactg ctgcgaattt
     7921 tttcatgttt attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa
     7981 caacttctta agggaaaaaa ataaaattta gtgctgtaca gagcgcgtta caacacggtt
     8041 tactggcagc aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga
     8101 tgacatgctg attggcgacg gcatcaaaac gggccttagt aaaatgggtt ttagcgtcga
     8161 ctggtttaca caaggtcgtc agggaaaaga ggcgctttat agcgcgcctt atgatgcggt
     8221 gatcctggat ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga
     8281 aaaaggtcag cgtgagccgg tactgatcct gaccgcgcgc gatgcgctgg aggaacgtgt
     8341 agaagggctg cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt
     8401 cgccgccagg ctggaagctc tgatgcgccg aaccaacggc caggccagca acgagctgcg
     8461 ccacggtaac gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt
     8521 aacactgaaa ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt
     8581 actgccgcgc aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa
     8641 tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac
     8701 cgtgcatggt attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg
     8761 cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc
     8821 tttgtcgcct ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg
     8881 ctgtttgcca agcggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg
     8941 gcacagacgc caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc
     9001 atctttaccc acgacggcag aatggtcctt aacgatggcg ataacggaga agatattcct
     9061 tatagctatc aacgggaagg ttttgctgac gggcaactgg tgggcgataa agatcaatgg
     9121 cgttttgtct ggatgacctc acctgatggc aaatatcgca tcgttgttgg tcaggaatgg
     9181 gaataccgtg aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc
     9241 gcactgccga ttatgttaat catcatgatg gtactattgg gtcgtgaact cgcgccgctg
     9301 aacaaactgg cgctggcgct gcgtatgcgt gaccctgact cggaaaaacc actaaacgcg
     9361 actggcgtac ccagcgaagt gcgtccgttg gttgagtcgc taaatcaact gttcgcccgc
     9421 acacatgcga tgatggttcg tgaacgacgc tttacctccg acgcagctca cgaactacgt
     9481 agcccgttaa cggcgctgaa agtgcaaacc gaagttgcgc agctttctga cgatgatccg
     9541 caagcgcgga aaaaagcgct gcttcaatta cattacggga tcgatcgcgc tactcgtctg
     9601 gttgatcaac tgctcactct atcgcggctg gactcactgg ataaccttca ggacgtcgcg
     9661 gagatcccgc ttgaagatct cctgcaatcg tcggtgatgg atatttacca cacggcgcag
     9721 caggcgaaaa ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa
     9781 ccgctattgc taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca
     9841 cagggcagcg tgatagacgt cacgctgaat gctgataatt tcatcgtgag ggataacggc
     9901 cccggtgtga cgccagaggc tctggcgcga attggcgaac gcttctatcg cccccccgga
     9961 caaaccgcta ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc caaattgcat
    10021 ggcatgaatg ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg
    10081 taacttctct gtctatgaga gccgttaaaa cgactctcat agattttact aatagcaaaa
    10141 tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga caaataccga gcgagaatac
    10201 atatacccaa aactcgaaca tttcccgcat aaagagtttt cttaagataa gaataataag
    10261 tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac
    10321 ggaaggtata ctgcaaatat tgtctcccga ctcaagcatc cagtcatact tattgaaagc
    10381 aaaaaaagca tctattaacc aataagtaag cattatgtat aacagagctt caaatattgt
    10441 ttgaatttta tgctttaaca tatatcagta cgctcataaa aatccattta taattatcaa
    10501 catgacacgt tgatattttc atcttttact tattcaaagc catattttca atcccaaaaa
    10561 aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg aatggctatc
    10621 agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagca agatcagact
    10681 tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc gctctgtgac
    10741 gtactatagt cggcaaacgt ctcaccttga ggttaaaaaa tgagcaacat cctgattatc
    10801 aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc
    10861 gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac
    10921 tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg
    10981 ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga tgtattcacc
    11041 gaaggtcatg gaacgctgta tgccagcgat ggccgtaccc gcaaagatcc gtcaaaaaaa
    11101 tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctgac ctggaacgca
    11161 ccaatggaag ccttcaccga aaaagatcaa ttcttccacg gcgttggcgt cgacggtgtg
    11221 tatctgccgt tccataaagc aaaccaattc cttggcatgg aaccgctgcc gacatttatc
    11281 gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat
    11341 cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac catgcttacc
    11401 gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag
    11461 tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg
    11521 gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt
    11581 gagcagtggg aaagcatcgc gcatcttgaa gcgcatctgc aaaccccgca catgaaggcg
    11641 tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgcatcct gcaacctggg
    11701 atttaaacct gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc
    11761 tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc
    11821 ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc
    11881 aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc
    11941 atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc
    12001 tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca
    12061 atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag
    12121 tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc
    12181 gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata
    12241 ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact
    12301 tcccaggtgt taaggatctt acctttcagt ggcatgatcg cctgatattc gcgatcgcgc
    12361 gcctgcttgg cagatccgcc tgcggagtca ccttccacaa ggaacagttc ggtacggtta
    12421 agatcctgcg cggtacagtc agccagtttg ccaggcagtg ctgggccgct ggtcagcttt
    12481 ttgcgcacca cttttttagc cgcacgcata cggcgctggg cgctggaaat cgccatctcc
    12541 gccagcagct ccgccgcctg aacgttctgg ttcagccaca ggataaaggc atctttcacc
    12601 acgcctgaaa cgaatgccgc gcattgacgc gaagagagac gctctttggt ctgcccggca
    12661 aactgcggat cctgcatttt caccgacaga acataggcgc agcgatccca gatatcttcc
    12721 gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc acgcatcgcg
    12781 tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt tgggataagg
    12841 ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc
    12901 acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa
    12961 ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg
    13021 gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc
    13081 ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa ggtttcatcc
    13141 ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa
    13201 tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg
    13261 ttaacttcta cgcgcttcga cagggcgtta accaccgaaa tccccacgcc atgcaggccg
    13321 ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc
    13381 agttcaaccg ccggtacacc ctcttccggg tgaatatcca ccggcatccc gcgcccatcg
    13441 tcaataactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc
    13501 gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg
    13561 gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg
    13621 gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag
    13681 atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc
    13741 gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccgcctggcg
    13801 gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag
    13861 cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg
    13921 cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa acgggcgcac
    13981 cgccgagttt accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata
    14041 tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga
    14101 ttccagcagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac
    14161 gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga
    14221 gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca
    14281 ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt
    14341 aactcgagag tacgccagcc gggcgcgatg gtgtccagcg taaagttgga acagtgcggc
    14401 ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc
    14461 agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg
    14521 gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca acccgcaggt
    14581 agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt
    14641 tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta
    14701 tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc
    14761 gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgccagg cagccagacg
    14821 cagggcgcac gaaaacttgc gatgccttca gcgaaatgcc gataggccgc agaggattga
    14881 tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc
    14941 gcctccagca ccgcctggta gctttcccag gtatttaccc ctaacagggc ttcgtgcttt
    15001 tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc accagccaga
    15061 ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga
    15121 atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt
    15181 tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag
    15241 cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc
    15301 gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg
    15361 gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag
    15421 cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc
    15481 atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac
    15541 tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg
    15601 ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg
    15661 cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact
    15721 tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac
    15781 accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca
    15841 tcttccacac tttcaccctc ttctatcatc ccggcaacca tctccagtag ccagggggtt
    15901 tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca gcacaacttc atcacgcact
    15961 gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca
    16021 tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag
    16081 ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc
    16141 aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt
    16201 tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac attaacgccc
    16261 tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt
    16321 acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc
    16381 ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga
    16441 tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc
    16501 gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt
    16561 taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc aactctaacg
    16621 cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt
    16681 taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa
    16741 ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt
    16801 cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt
    16861 ttaacgtggg cctggtagcg ataaccgacg tgcagaacgc ccgcgcgcag tacgataccg
    16921 tgctggcgaa cgaagtgacc gcacgcaata accttgataa cgcggtagag cagctgcgcc
    16981 agatcaccgg taactactat ccagaactgg cagcgctgaa tgtcgaaaac tttaaaaccg
    17041 acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt
    17101 tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc
    17161 acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt
    17221 cgaaaacccg tggtgccgct ggtacccagt atgacgacag caatatgggc cagaacaaag
    17281 tgggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg caggtgaaac
    17341 aggcacagta caactttgtc ggtgccagcg agcaactgga aagcgcgcat cgtagcgtcg
    17401 tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcatc tatcagtagc attaacgcct
    17461 acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg ggctactcgg
    17521 tcggtacgcg taccattgtt gatgtgttgg atgcaaccac cacgctgtac aacgctaagc
    17581 aagagctggc aaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc
    17641 tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc aaaccggttt
    17701 ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt
    17761 atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta
    17821 acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt
    17881 aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg
    17941 accagccatc ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac
    18001 ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcgcgc catctgacac
    18061 cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa
    18121 cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg
    18181 aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg
    18241 ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcag gcaccagccc
    18301 aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg agtttctgga
    18361 tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac
    18421 agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa tataccgacg
    18481 cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacag
    18541 ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg
    18601 ttgccaaaca aagcactatg cagcgtagcg caactggcac ctcttctcgt tcaatgggtg
    18661 gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca
    18721 cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta
    18781 ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca
    18841 gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg
    18901 cattccaaaa cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc agccatcgct
    18961 ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa
    19021 cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga
    19081 tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa
    19141 actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac
    19201 ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcaac
    19261 ggaagctgaa attgctactg agttcctcta catcgatgat atcgggttag gtgaaaaagg
    19321 tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt atccgtggga
    19381 atttatgttg cgtgagatgt tttccaccaa gctggaggat gcaggcgtac gctggctgga
    19441 accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt
    19501 cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga
    19561 aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgaaaa
    19621 cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca
    19681 attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa
    19741 cgatcaaccc gccggaattg gcattcgtga agaccgtgca ttgatcaccc aggatatgtc
    19801 tcggttttat ccacatattt ttgttgagta atcaatgatg ccggatggca ttcgccatcc
    19861 ggtaattgtt aacctatctg caccgacagc atactcaggc tgcccatttc tataccatcg
    19921 accggaatgg taattggctc ctgcccatcc cacgcaccta agacatacaa caacggcaga
    19981 tagtgctccg gcgttgggtt cgataacgcg ccaccttcat ggtcgaggta attcaccaga
    20041 ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc attaaacgac
    20101 atcgcccaag gatatggtga actatcaccg tgccacttca ctgtacgcag gttatgcacc
    20161 acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag tttgcgcccc
    20221 atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata
    20281 tcggcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa gccccaggct
    20341 tctttatcca gcgtcaccgg gaccggtgct aacagctcaa ccagatgctg tgccagcaca
    20401 ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca
    20461 tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc
    20521 gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg
    20581 gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc
    20641 atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa
    20701 tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg
    20761 acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg
    20821 accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa
    20881 ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc
    20941 tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc tgtgttgggt
    21001 tatggaatgt ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccacat
    21061 gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc
    21121 acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc
    21181 tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg
    21241 cacaatattc ctgaaggtct ggcggttgca ggcccggttt atgcagcaac gggttctaaa
    21301 cgcacggcaa ttctgtgggc ggggatttct ggattggcag aaattcttgg tggtgtgctg
    21361 gcgtggttaa tcctaggtag catgatttcc cccgtggtca tggcggcaat catggcggcg
    21421 gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt
    21481 gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt
    21541 ttagtgctgc tacaaacggc gggaattggt taaaagcaac atcagaagtt ggccagcgca
    21601 tggacaactt ctgctagctt cactaaagcc ttattctacc ttttcggctg tctcttctct
    21661 cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc
    21721 ccgacaataa cgtttcaaat gcaggaaata acccaccaca acaagagcaa tcaatacaat
    21781 ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc
    21841 gcataaattt cacatatcct ttttcgg
//
LOCUS       DAZUFB010000081        21622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZUFB010000081 DAZUFB010000000
VERSION     DAZUFB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.081"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..539
                     /gene="ftnA"
                     /locus_tag="ACHY3M_003389"
     CDS             42..539
                     /gene="ftnA"
                     /locus_tag="ACHY3M_003389"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFS4318241.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(577..816)
                     /gene="yecH"
                     /locus_tag="ACHY3M_003390"
     CDS             complement(577..816)
                     /gene="yecH"
                     /locus_tag="ACHY3M_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFS4318242.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1007..2218
                     /gene="tyrP"
                     /locus_tag="ACHY3M_003391"
     CDS             1007..2218
                     /gene="tyrP"
                     /locus_tag="ACHY3M_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFS4318243.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     IALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2280..2945)
                     /gene="yecA"
                     /locus_tag="ACHY3M_003392"
     CDS             complement(2280..2945)
                     /gene="yecA"
                     /locus_tag="ACHY3M_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFS4318244.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3141..3227)
                     /locus_tag="ACHY3M_003393"
     tRNA            complement(3141..3227)
                     /locus_tag="ACHY3M_003393"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3191..3193),aa:Leu,seq:taa)
     gene            complement(3240..3313)
                     /locus_tag="ACHY3M_003394"
     tRNA            complement(3240..3313)
                     /locus_tag="ACHY3M_003394"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3279..3281),aa:Cys,seq:gca)
     gene            complement(3368..3443)
                     /locus_tag="ACHY3M_003395"
     tRNA            complement(3368..3443)
                     /locus_tag="ACHY3M_003395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3408..3410),aa:Gly,seq:gcc)
     gene            complement(3595..4143)
                     /gene="pgsA"
                     /locus_tag="ACHY3M_003396"
     CDS             complement(3595..4143)
                     /gene="pgsA"
                     /locus_tag="ACHY3M_003396"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4318245.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4200..6032)
                     /gene="uvrC"
                     /locus_tag="ACHY3M_003397"
     CDS             complement(4200..6032)
                     /gene="uvrC"
                     /locus_tag="ACHY3M_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4318246.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKVGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6029..6685)
                     /gene="uvrY"
                     /locus_tag="ACHY3M_003398"
     CDS             complement(6029..6685)
                     /gene="uvrY"
                     /locus_tag="ACHY3M_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFS4318247.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEVSCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6827..6898
                     /locus_tag="ACHY3M_003399"
                     /pseudo
     CDS             6827..6898
                     /locus_tag="ACHY3M_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6981..7157
                     /gene="yecU"
                     /locus_tag="ACHY3M_003400"
     CDS             6981..7157
                     /gene="yecU"
                     /locus_tag="ACHY3M_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFS4318248.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7144..7368
                     /gene="yecF"
                     /locus_tag="ACHY3M_003401"
     CDS             7144..7368
                     /gene="yecF"
                     /locus_tag="ACHY3M_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFS4318249.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7435..8157)
                     /gene="sdiA"
                     /locus_tag="ACHY3M_003402"
     CDS             complement(7435..8157)
                     /gene="sdiA"
                     /locus_tag="ACHY3M_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFS4318250.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8387..9139)
                     /gene="tcyN"
                     /locus_tag="ACHY3M_003403"
     CDS             complement(8387..9139)
                     /gene="tcyN"
                     /locus_tag="ACHY3M_003403"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFS4318251.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9136..9804)
                     /gene="tcyL"
                     /locus_tag="ACHY3M_003404"
     CDS             complement(9136..9804)
                     /gene="tcyL"
                     /locus_tag="ACHY3M_003404"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFS4318252.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9819..10805)
                     /gene="dcyD"
                     /locus_tag="ACHY3M_003405"
     CDS             complement(9819..10805)
                     /gene="dcyD"
                     /locus_tag="ACHY3M_003405"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFS4318253.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10910..11710)
                     /gene="tcyJ"
                     /locus_tag="ACHY3M_003406"
     CDS             complement(10910..11710)
                     /gene="tcyJ"
                     /locus_tag="ACHY3M_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4318254.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVTLRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11798..12349)
                     /gene="fliZ"
                     /locus_tag="ACHY3M_003407"
     CDS             complement(11798..12349)
                     /gene="fliZ"
                     /locus_tag="ACHY3M_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFS4318255.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12395..13114)
                     /gene="fliA"
                     /locus_tag="ACHY3M_003408"
     CDS             complement(12395..13114)
                     /gene="fliA"
                     /locus_tag="ACHY3M_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFS4318256.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13279..14757)
                     /locus_tag="ACHY3M_003409"
     CDS             complement(13279..14757)
                     /locus_tag="ACHY3M_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFS4318257.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGKTYTVNVESGAVKNDA
                     NKDVFVSAADGSLTTSSDTKVSGESIDATELAKLAIKLADKGSIEYKGITFTNNTGAE
                     LDANGKGVLTANIDGQDVQFTIDSNAPTGAGATITTDTAVYKNSAGQFTTTKVENKAA
                     TLSDLDLNAAKKTGSTLVVNGATYNVSADGKTVTDTTPGAPKVMYLSKSEGGSPILVN
                     EDAAKSLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSR
                     IEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            15012..16425
                     /gene="fliD"
                     /locus_tag="ACHY3M_003410"
                     /pseudo
     CDS             15012..16425
                     /gene="fliD"
                     /locus_tag="ACHY3M_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
     gene            16440..16850
                     /gene="fliS"
                     /locus_tag="ACHY3M_003411"
     CDS             16440..16850
                     /gene="fliS"
                     /locus_tag="ACHY3M_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS4318258.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            16850..17215
                     /gene="fliT"
                     /locus_tag="ACHY3M_003412"
     CDS             16850..17215
                     /gene="fliT"
                     /locus_tag="ACHY3M_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFS4318259.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17293..18780
                     /gene="amyA"
                     /locus_tag="ACHY3M_003413"
     CDS             17293..18780
                     /gene="amyA"
                     /locus_tag="ACHY3M_003413"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS4318260.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18814..19227)
                     /gene="yedD"
                     /locus_tag="ACHY3M_003414"
     CDS             complement(18814..19227)
                     /gene="yedD"
                     /locus_tag="ACHY3M_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFS4318261.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVNTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19414..20619
                     /gene="yedE"
                     /locus_tag="ACHY3M_003415"
     CDS             19414..20619
                     /gene="yedE"
                     /locus_tag="ACHY3M_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFS4318262.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQLCFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            20616..20849
                     /gene="yedF"
                     /locus_tag="ACHY3M_003416"
     CDS             20616..20849
                     /gene="yedF"
                     /locus_tag="ACHY3M_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFS4318263.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVMSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            20956..>21622
                     /gene="yedK"
                     /locus_tag="ACHY3M_003417"
     CDS             20956..>21622
                     /gene="yedK"
                     /locus_tag="ACHY3M_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFS4318264.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
BASE COUNT         5383 a         5454 c         5213 g         5572 t
ORIGIN      
        1 tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga
       61 ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa
      121 tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg
      181 cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt
      241 taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat
      301 tccaggaaac ctataaacac gaacaattaa tcacgcagaa aattaacgaa ctggctcatg
      361 ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat gtttctgagc
      421 agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa
      481 gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat
      541 gctctcaggc ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg
      601 atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac
      661 cagctctccc gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa
      721 acgcgcttta attgctgctt ccagactggc atgcgtatat tgctcgcctg attcaatcat
      781 catatttaac acttcatgac cgtgaataga gtccatcgtc cctcctcaaa aaaagcctag
      841 cgtagcgatt gccgcttatg aagactttgc gccagcgcag gactgaatgc tttttattgt
      901 acatttatat ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc
      961 tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac
     1021 cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc
     1081 gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc
     1141 gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac
     1201 cggtcttggc acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt
     1261 cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt
     1321 gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct
     1381 gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt
     1441 taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct
     1501 accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc tggctctgtc
     1561 agcaatcccg gtgattttta cctcatttgg ttttcacggt agcgtgccga gtattgtcag
     1621 ctatatggat ggcaacgttc gtaagctacg ctgggtgttt ataaccggta gtgcgatccc
     1681 cctggtggca tatattttct ggcaggtggc aacgcttggc agcattgatt caacaacctt
     1741 tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat
     1801 ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac
     1861 gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttat ttcagcgttc
     1921 aaataccgtt ggtggacggt tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt
     1981 tgcactgttt tatccacgag gatttgtgat tgcgctgggt tacgccggtg tggcgctggc
     2041 ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcagaaagc acaatcctca
     2101 ggcgggttac cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat
     2161 tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc
     2221 agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttgacctct
     2281 taatgcaggc agcactgctt aaatttctta ccactaccgc acgggcaagg atcgttacgc
     2341 cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc
     2401 cagtacgcat gtagatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct
     2461 tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc
     2521 tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc
     2581 atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag
     2641 ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata
     2701 tgttgaaaag ccagattcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca
     2761 tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa
     2821 ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg
     2881 ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt
     2941 ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt
     3001 atcagagata ctttttgagt ggctttgctg gtgattaaaa attagggagg gtgtaacgac
     3061 aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga
     3121 ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg
     3181 ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt
     3241 ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct
     3301 ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct
     3361 tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
     3421 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
     3481 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
     3541 tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg
     3601 atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
     3661 cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag
     3721 cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc
     3781 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
     3841 acgggcgatc atcgttgccg ccggtagggt cacccaccag ctgtgataat gctcggttac
     3901 cagcaccatg gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa
     3961 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
     4021 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa
     4081 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
     4141 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
     4201 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
     4261 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
     4321 atttgccgac gttttggccc gacgccttca atggtttcca gggagctggt atttttgacc
     4381 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc
     4441 tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca
     4501 aagaacagtg tttccagccc aaccttacga tctactcctt tggcaacgcc aagtagcagt
     4561 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
     4621 tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta
     4681 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct
     4741 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
     4801 gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact
     4861 tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc
     4921 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc
     4981 ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
     5041 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
     5101 cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt
     5161 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
     5221 atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg
     5281 tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
     5341 tcgcgaatac gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta
     5401 atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
     5461 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
     5521 atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
     5581 atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac
     5641 gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg
     5701 gtatcaccac tcaggaagat aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa
     5761 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtgtga
     5821 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
     5881 aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
     5941 ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt
     6001 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
     6061 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
     6121 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
     6181 cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac
     6241 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga
     6301 tgtcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct
     6361 gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta
     6421 aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga
     6481 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
     6541 caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatacc tcaccgacga
     6601 cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
     6661 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
     6721 tagttagtca ttcgtatatt agctgttttt attgcaactt tgaaacgtta aaaatggtgg
     6781 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
     6841 caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg
     6901 actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
     6961 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgtatttt
     7021 atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
     7081 tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa
     7141 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
     7201 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
     7261 cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca
     7321 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
     7381 tgagagcgtg cctttcagcc ggtttttgca tctggcacgc agaaagaaaa gcgatcaaat
     7441 taagccagta gcggccgcgt aacaggccac ctgggtctta tttggtgcat tgattttttt
     7501 ctgcatgttt ttctgatgga aattgaccgt attctcagag attgacaaaa tcatcgctat
     7561 ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa
     7621 attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc
     7681 gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt
     7741 gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt
     7801 gacaccgcgg cgtaaaccat gtgcgcgcgc ggcttcccat aacggctgtg cttcgctgaa
     7861 taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca gcaccggatc
     7921 aattgcgaga aagttttttg cctgataata actaacccac gcctcagggt aattggtgta
     7981 gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc
     8041 gtactccagc tgctgagcct gaagctctat ttcatggtag acctcttctg cggcctccat
     8101 cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt
     8161 aaaccgcaac gcccctgaga gtgagtatca tttataatga taattcaaac tatcacataa
     8221 ataagattta tatataattt atattattca ggcaatgaat tacttttgca agccatcgca
     8281 ttctcttatg ttattaatga gttatgctga tttgttaagc agttttatca cgcttgaaat
     8341 ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa
     8401 acttctcgag gaactggcgg gtgcgaggct gctggggatc ggcaaataac gcttttgctg
     8461 ccccctgctc gactatccgc ccctggtcca taaagatcgc ccggtccgca acatcccggg
     8521 caaagctcat ttcgtgtgtc acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga
     8581 tggtgttcag gacttcgccc accagctctg gatccagcgc cgacgttggc tcgtcaaaca
     8641 aaatcacctc aggacgcatt gccagtgcac gcgcaatcgc aacacgctgc tgttgaccgc
     8701 cagacaaacg acgtggatag ctggtttctt taccggccag cccaactttc gccagcagct
     8761 cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa
     8821 taatgttttc cagcaccgta cgatgcggaa acaaattaaa gttctggaag acaaacccga
     8881 cgtgctgacg caactggcga atcagcgatt tttgctgact taatgaacgt gcagtatcaa
     8941 tagtgatatc gccaacggtg atcgttcccg cttccggttg ttccagcaga tttatgctgc
     9001 gtagcaacgt ggttttgccg gaaccactcg gaccgataat tgccaccact tcgccaggct
     9061 ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg aaattttttc accaggttct
     9121 taacttcaat ggcactcatt ttggttctct ctcctggcga ttaagttgat tctcaaaatg
     9181 gttctgcaac gtcgataata ctgtcgccat gatccagtag atgagcgaag ccgccaggta
     9241 catggtgaac acctccagcg tgcgcgaggt aatcaactgc gcctgacgga acagctccgg
     9301 cacctggatt gtcgcggcca gcgacgtatc ttttaccaga ctgataaagc tgttcgacag
     9361 cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg
     9421 cgtcatacca atactggcag cggcttccca ctgaccttta tcaatagaag aaatggcagc
     9481 gcgcagcgtt tcggcggcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg
     9541 aatgggatct aattcgatac caaactgcgg caggccgtaa tagatcataa acagttgtgc
     9601 gatgagcggc gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca
     9661 gattggcgac aggcgcatca gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc
     9721 gccgatactg agttgcaggg tatacccagc ccctttcaac aggaacggca aggaatcaat
     9781 aaccagttgt atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg
     9841 aacagcgcag gcgcgccgcc ggtatgaata aacagaatcg gcccttcatc tttgaagcgt
     9901 ttctgactga taccgtcaat cagccccgcc atcgcttttc cggtatacac aggatcaagc
     9961 agaatgcctt ccagacgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg
    10021 ccgtagccag gtgcaaaata gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc
    10081 agttctttcg caatcgcctg ttgtaggtta accactttcg gcaattgatc ggcaacggaa
    10141 cgcgacacgg tcacgccaat cagttcgctt tcaggcatca ggtgttccag cccaacagcc
    10201 aatccagcgt gagttccggc actgcccgat gcgaccacca ccgacgaaat attaaccgcc
    10261 ccttcacact gttgcgcgat ttccagcgca ctctccacat aacccagcgc gcccagagca
    10321 ttagaaccgc caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc
    10381 agctcttcca gttgggcatt gggatcggtc agtgcgtcgc acatttcaat ctgggtattg
    10441 aacagatcca gcaacaaacg attgccgttg gttaaatagt tttctgcggt tgtgccaata
    10501 ggattttcca gcagcgccac gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg
    10561 cgcacatggt tagactggat cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg
    10621 gcatctgccg cgagaaattc cagcttacgt aatttattgc cgcccattgc catgggtgtg
    10681 acgtcatccc gtttgatgaa aatttcccgt ccaagataat cagaaaagcg cggcagatat
    10741 tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa aacgggttaa attatgcagt
    10801 ggcataacag cctccgatgt gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg
    10861 taaataaaaa aggcgctagt gaaagcgccc ttttttgtca ttatgctgat tatttggtca
    10921 catcagcacc aaaccatttt tcggaaagag cttgcagagt gccgtctttt tgcatttccg
    10981 caattgcatc attcaccgct ttcagcaggt cttcatttcc tttacgcagc gtcacgccag
    11041 actcctgacg ggagaatgct tcaccggtta ctgccagcgt atcgttggtt ttcttcacca
    11101 gatccagcgc cgccagacga tcaacgagga tcgcatcgat acggcctacg cgcagatcct
    11161 gatatttggt cgggtcatca tcataggtac gcacatcgac gccctgaaca ttctgccgca
    11221 gccactcttc atagttggtg cccagaccga cacccacttt tttgcctttc agatcagcag
    11281 ccgttttaat ggtgccttcg ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt
    11341 aaggggtaga gaaatcgtat tttttcttgc gctcgtcaga aatggtgacc tgattaatca
    11401 ccacatcaat gcgtttagag tccagcgacg ccagcatacc gtcccatttg gtcggtttta
    11461 gtgacgcctc aacgccaaga tgttttgcca gctgttgggc aaattccact tcaaaaccgg
    11521 ttaatttgcc gtcatctccc tgaaaactga acggcggata agtgccttcc agccctacca
    11581 gcagcgtgcc gcgctcttta actttattaa gcagaccttc atctgcaaaa cttttaacgc
    11641 tcatgcccgc aaccagcgct acggccatca cacccatcaa tgcctgacgt cccagatgtg
    11701 ctaatttcat atttaccccg aatgttgtta tgtctgtttg cagtgtagag ccattgtttg
    11761 taaacacaaa aacaactccg ctacatctta ttcttattta atatatatca gaagcaggca
    11821 ggctggagga tttctgcaca agtccggtac acgtttgacg ctgatagtgt tgatacttcc
    11881 gtaatgcgat gcggtaattg ttggtgctgg tggtgggcag ccacggggcg aggatttcat
    11941 caaggaaacc gtcctgcagc ggatcgaggg aaatattttg ctcgtgtaga tgattcccca
    12001 aacggcgcag acgaacgaca tattcacgca ccgtaccagg gctcatttca gtttgctcaa
    12061 aaagaaattg cttaaagccg ataatatcga aaaaatcact ctgcgttttg caatgtaaat
    12121 caccgcagaa tcggcacaat gccgcccatg atttttgttc cacttgccag ccattttcat
    12181 caagcagcat gtccaggcgg gaaatctcga ttttattcac tattttgccg tcgcgaacga
    12241 gggtaatgcg gtcgagtaat ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt
    12301 ctttaagata gcggcttaat ggccgtcttt tcaggtgctg caccatcatt aagaactcct
    12361 ggtagtcaaa attaaagtgc ggcatttact gacgttataa cttacccagt ttagtgcgta
    12421 accgtttaat ggcctggctg tgtaactgac tgacccgcga ttccccgacc tccagcaccg
    12481 cgccaatctc tttgagattc agctcttcct ggtaatagag ggttaatacc agtttttcgc
    12541 gctccggcaa cgtttcgatg gcttccatca cccgctggcg cagattactg tccagcagtt
    12601 gttgtagcgg gttttctcgc tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct
    12661 cttcgcgcca ctcatcgtag gagaagagct ggctgttatt ggtgtcgagc aacatttggc
    12721 gataatcggc aatatcgatc cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc
    12781 caagttcctg ctccagttgc cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc
    12841 tgcgcggcac ccagtcacgg ctgcgaagtt catccagcat agcgccacgg atacgctgca
    12901 ctgcgtaagt tgtaaatgcc gttccttgta gggcgtcata gcgttcgacg gcattaagta
    12961 acccaatgcc gcccgcctgt agcagatcgt caagttccac gctcgcgggc agtcgaacct
    13021 gcaggcgcaa tgcttcgtga cgcaccagcg ggacataacg ctgccacagc gagtgtttat
    13081 ccattacacc ttcagcggta tagagtgaat tcacgataaa cagccctgcg ttatatgagt
    13141 tatcggcatg attatccgtt tctgcagggt ttttaatcgg acgattagtg ggtgaaatga
    13201 ggggttattt gggggttacc ggtaaattgc ggacagaaaa aaccccgccg gtggcggggt
    13261 ttgagcgata agtgtaaatt aacgcagcag agacagtacg ttctgcgtgg tctggttagc
    13321 ctgcgccaga acagaggtac ccgcttgttg caggatctgc gcacgagaca tgttagacac
    13381 ttcggtcgcg tagtcagcat cttcgatacg gctacgggca gaagacaggt tgtttacggt
    13441 gttgccaagg ttggtgatgg cagagtcgaa acggttttgt actgcaccga ggtcagaacg
    13501 cagattgtca actttagcca atgccttgtc gatagtttcg agcgggttgg tggtagattg
    13561 caacgatttt gctgcatctt cgtttaccag aatcgggcta ccaccttctg atttgctcag
    13621 atacatcact ttaggggcac caggagtagt atcagttacc gttttaccat ctgcgctgac
    13681 attgtaggtg gcgccattta caactaaagt gctacctgtt ttcttggctg cattaagatc
    13741 cagatcagag agtgttgcgg ctttattttc cacttttgta gtggtgaact ggcccgcact
    13801 gtttttgtaa acagctgtgt ctgtagttat tgttgcgccg gcacccgtgg gtgcattact
    13861 gtcaatagta aattgaacat cttgaccatc aatatttgcg gtcaaaacac ctttaccatt
    13921 agcatcaagc tctgcgccag tgttgttagt aaatgtaatg cccttgtatt caatggagcc
    13981 tttgtcagct aattttattg caagtttcgc tagttctgtt gcatcaatac tttcaccgga
    14041 tactttagta tcactactgg tcgtcagcga tccatcagct gcgcttacaa aaacatcttt
    14101 attagcatca ttcttaaccg cgccgctctc cacattcacg gtataagttt taccgccaac
    14161 atcataatta tcagtacctg tcgctttaaa tttagaaatc aggtcactgc ctgttgcttt
    14221 ctgcgcgcca tcgatattaa aaccgtccag gccgagagtt ttcgcatcaa tttttgccag
    14281 attgatagtg atggtttcac catcattagc accaacctga attttcattt cattattttc
    14341 agcaaggact ttcacgccgt taaactgagt ttgctcagat acacggtcaa tttcttccag
    14401 acgttgagta atttcagcct ggatagaaga aagatcgctg tcagagttag taccgttagt
    14461 tgcctgaaca gaaagttcac gaatacgctg caggttgttg ttaatttcat tcagcgcacc
    14521 ttcagtggtc tgcgcaacag aaataccatc atttgcgtta cgggaagcct gggtcagacc
    14581 tttaatattt gccgtaaaac ggttagcaat cgcctgacct gctgcatcgt cttttgcgct
    14641 gttaatacgc agaccagaag acagacgctc aatagcagag ctaagagaag actgagattt
    14701 gttcaggtta ttctgggtca acagcgacag gctgtttgta ttaatgactt gtgccatgat
    14761 cttttcctta tcaattacaa cttgatgtta ttgggctgtt gcccacggtt tctcaccgta
    14821 ccccttgtat cggcacctga atttcgaact ttagaaaatt ttagtttctc tcctgttttt
    14881 tcttaacggc tgaaatatcc gtaatataca actctactct gcgcatcatc ttcgtgaatc
    14941 aactattaaa ctttgcgcac aaattgccga taacccgctt atctactgtt tgcaatcaaa
    15001 aggaattagg tatggcttca tttacatctc ttggcgttgg ttcgaatctg ccgctggata
    15061 cattgctaaa caacctgacc atcgctgaaa aaaaacgctt aaacccaatt actcagcaac
    15121 agagtgacaa taccgcacgt ctgacagcct atggcacatt aaaaatgtgc attagaaaaa
    15181 ttccagactg caaatactgc gttaaataaa gccgatctgt tcaaaagcac taccgttacc
    15241 agtagcaatg aagaccttaa agtttccacc gaagcaggtg ctgcccccgg tatctatacc
    15301 atcagcgtaa cccaactggc gcaggcgcaa tctttacgaa ctgattcccc gacaatcatt
    15361 gcatcaacaa aagatgcact gggtgatgag tcatcagata cacgcacaat aaaaattacc
    15421 caagacggcc gtaaagagcc gctggaaatc aagctcaata aagatcagac gtcattggat
    15481 gagatcagca aagccattaa cgatgccgat agtggaattt ccgctagtat cgttaaagtc
    15541 aaagatggtg actatcaatt ggtattgacc gccagtgaag gtctggccaa taaaatgacc
    15601 atatctgttg agggtgacag taaattaaat gatctgctgg cttatgacag caagaccaac
    15661 accgggaata tgaaggaact ggttaacgcg caaaatgcac agctaaacgt taacggtatc
    15721 gatattgaac gttcaagtaa caaaattacc gatgctcctc agggagtgac tctcgatttg
    15781 accaaaaaag tcactgacgt ccgggtcacg gtgacaaaaa gcaacgataa agcaaccgaa
    15841 gcaattaaag gctgggtgga ttcatataac tccctcatta acacctttaa caccctgaca
    15901 aaatataaag aagtagaccc aggtgctgaa gcacaagata aaaataatgg tgcacttctc
    15961 ggagacagcg ttgtacgtac aattcaaagc ggcatccgcg cacaatttgc aaacggtgcc
    16021 agcgacggtg ccttcaaaac attaaacgag atcgggatta agcaggacgg cacgacgggt
    16081 aagctgaaaa ttgatgatga taaactcaaa aaagtcctga atgaaaatac agcatccgta
    16141 cgtgaattgc tggtaggcga tggtaaagaa acaggaatta ccaccaaaat tgccaccgag
    16201 gtaaaaggtt accttgccga tgatggcatt attgatagcg cacaggacag tattaacgcc
    16261 acactgaaaa aactgaccaa acagtatcta tccgtcagtg ccagcattga tgacaccgtg
    16321 gcgcgttaca cggcacagtt tacgcaatta gataccatga tgagcaaact gaataacacc
    16381 agtacctacc tcagccaaca atttactgca atgagtaatt cctgatcacg gaggtcagca
    16441 tgtataccgc aagcggctca aaagcctatg cacaaattgg cgtcgaaagc gccgtaatga
    16501 gcgccagtca acagcagctg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    16561 gagcgcgcct gtttatgcag gacaacaatc aacaaggcaa aggcgtctca ttgtcaaaag
    16621 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    16681 taacccaaaa cttgattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    16741 tacgcaacga tgtctccgca gtcgaagaag tggaaacatt aatgcgcaat attgccgatg
    16801 cctggaaaga gtcgttactc tccccttctt tgattcagga cccagtctga tgaaccatgc
    16861 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    16921 ggcaacggaa gaacaatggg acgaactcat cgccagcgaa atggcgtatg ttaatgcggt
    16981 gcaggagatt gcacatttga ctgaagagat tgacccgtcc accacgatgc aggagcagct
    17041 ccgcccgatg ctgcgcctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    17101 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    17161 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctg
    17221 aatgagtcga tggctcgcga ataatccgat tacggctacg cttctaatgt tccccttgaa
    17281 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    17341 ggcggtaagc tctggcctga actggccgag cgcgccgacg gttttaatga tattggtatc
    17401 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcgggtattc ggtcggctac
    17461 gactcctatg atttatttga tttaggcgag tttgatcaga aaggcagcat ccccactaaa
    17521 tatggcgata aagcacaact gctggccgcc attgatgctc tgaaacgtaa tgacattgcg
    17581 gtgctgttgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    17641 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    17701 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    17761 ttcaaatgtt ttagcggtat cgaccatatc gaaaaccctg atgaagatgg catttttaaa
    17821 attgttaacg actacaccgg cgaaggctgg aacgatcagg ttgatgatga attaggtaat
    17881 ttcgattatc tgatgggcga gaatattgat tttcgcaatc atgccgtgac ggaagagatt
    17941 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggttttcg tcttgatgcg
    18001 gtcaaacata ttccggcgtg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    18061 ccaaagccgc tgtttattgt ggcagagtac tggtcgcatg aagtcgataa actgcaaacg
    18121 tatattgatc aggtggaagg caaaaccatg ctgttcgatg cgccgctgca gatgaaattc
    18181 catgaagcat cgcgcatggg gcgtgactac gacatgacgc agattttcac gggtacatta
    18241 gtggaagccg atcctttcca cgccgtgacg ctcgttgcca atcacgacac ccaaccgttg
    18301 caagccctcg aagcgccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    18361 ttgcgggaaa atggcgttcc ttcggtattc tatccggacc tctacggtgc gcattacgaa
    18421 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    18481 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacgttatt tttcgaccat
    18541 ccgaactgca ttgcctttag ccgcagtggc actgacgaat atcccggctg cgtggtggtc
    18601 atgtcgaacg gggatgatgg cgaaaaaacc attaatctgg gagagaatta cggcaataaa
    18661 acctggcgtg attttctggg gaaccggcaa gagagtgtag taaccgacga aaacggtgaa
    18721 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    18781 attcatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    18841 tttatccagc gcggcagcac actctgctgt cgggcgatcc acacgctcca tcgtcatacc
    18901 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    18961 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatca cccgctgcca
    19021 ctgacggcaa tccagagtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    19081 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    19141 gggggtgttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    19201 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    19261 ggcattaaag ccctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    19321 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcgactctt
    19381 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    19441 tggttgatta aattctgggc gcccatcccc gcggtcatcg cggcgggtat tctctccact
    19501 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    19561 ggccagctcc tgcaactgtt cggcgtccat gctgaagagt ggggttattt taaaattatc
    19621 catctggaag gatcgccatt aacccgcatc gacgggatga tgatcctcgg tatgtttggc
    19681 ggctgctttg ccgcagcgct gtgggccaac aatgtcaaac tgcgaatgcc gcgcagccgt
    19741 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    19801 atgggctgta acctggcggc gttctttacc ggtattcctc agttctcgct gcatgcctgg
    19861 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    19921 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    19981 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    20041 tgggcgctgc tcacggcgat gaaccaaccc aaactggggc tggcaatgct gtttggcgtc
    20101 ggctttggtt tactgattga acgtgcgcaa ctctgcttta cttcagcgtt ccgcgatatg
    20161 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta ttggtatggc ggtgagtgcc
    20221 atcgggatct tcagttacgt acagttaggc gttgaaccca aaatcatgtg ggcgggacca
    20281 aacgcggtaa ttggtggttt actgtttggt tttggcatcg tgctggcagg cggctgcgaa
    20341 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    20401 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    20461 accgactggg acaaaatcaa cctgctgaaa acctttggcc cgatgggtgg cctgctggtg
    20521 acatatttgc tgttgtttac tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    20581 cgccgtgcgg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    20641 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaactctt gaggcgatgc
    20701 cgcagttaaa aaaaggggaa atcctggaag tgatgagcga ctgtccacag tcgatcaata
    20761 atattccact ggatgcgcgt aatcacggct atacggtgct ggatattcag caagacgggc
    20821 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    20881 acatcgttgc ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    20941 tctcaggagg tttctatgtg tggacgcttt gcccaatccc aaacgcgtga agattacctt
    21001 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    21061 tacaacgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    21121 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    21181 gcccgcgtag aaactgcggc caccagtcgt atgtttaaac cgctctggca acatggtcgg
    21241 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaacagcct
    21301 tattttatct atcgcgctga tggacaacct gtttttatgg ccgcgatagg tagcacaccg
    21361 tttgagcgtg gtgacgaagc cgaaggattt ttaattgtca ctgctgcagc agaccagggt
    21421 ctggtagaca ttcatgaccg ccgcccactg gtactgtcgc cagaagctgc gcgggaatgg
    21481 atgcggcaag agattggcgg taaagaagca tcagaaattg ctaccaacgg ctgtgtgcca
    21541 gcaaaccagt tcacctggca cccggtatcg cgcgcggtag gtaatgtcaa aaaccagggc
    21601 gcggagttaa ttcaacctgt tt
//
LOCUS       DAZUFB010000082        21444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZUFB010000082 DAZUFB010000000
VERSION     DAZUFB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.082"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..298
                     /locus_tag="ACHY3M_003418"
     CDS             <1..298
                     /locus_tag="ACHY3M_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4318265.1"
                     /translation="FRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQA
                     EISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(341..1492)
                     /gene="ydiO"
                     /locus_tag="ACHY3M_003419"
     CDS             complement(341..1492)
                     /gene="ydiO"
                     /locus_tag="ACHY3M_003419"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFS4318266.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKVYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1506..3100)
                     /gene="ydiF"
                     /locus_tag="ACHY3M_003420"
                     /pseudo
     CDS             complement(1506..3100)
                     /gene="ydiF"
                     /locus_tag="ACHY3M_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3244..4002)
                     /gene="aroD"
                     /locus_tag="ACHY3M_003421"
     CDS             complement(3244..4002)
                     /gene="aroD"
                     /locus_tag="ACHY3M_003421"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4318267.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHETPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4033..4899)
                     /gene="ydiB"
                     /locus_tag="ACHY3M_003422"
     CDS             complement(4033..4899)
                     /gene="ydiB"
                     /locus_tag="ACHY3M_003422"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFS4318268.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4911..6176)
                     /gene="ydiN"
                     /locus_tag="ACHY3M_003423"
     CDS             complement(4911..6176)
                     /gene="ydiN"
                     /locus_tag="ACHY3M_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318269.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6403..7617)
                     /gene="ydiM"
                     /locus_tag="ACHY3M_003424"
     CDS             complement(6403..7617)
                     /gene="ydiM"
                     /locus_tag="ACHY3M_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318270.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYEQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7717..8073)
                     /gene="ydiL"
                     /locus_tag="ACHY3M_003425"
     CDS             complement(7717..8073)
                     /gene="ydiL"
                     /locus_tag="ACHY3M_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFS4318271.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8209..8316)
                     /gene="rprA"
                     /locus_tag="ACHY3M_003426"
     ncRNA           complement(8209..8316)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHY3M_003426"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8502..9614)
                     /gene="ydiK"
                     /locus_tag="ACHY3M_003427"
     CDS             complement(8502..9614)
                     /gene="ydiK"
                     /locus_tag="ACHY3M_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFS4318272.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVVQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10003..13059
                     /gene="ydiJ"
                     /locus_tag="ACHY3M_003428"
     CDS             10003..13059
                     /gene="ydiJ"
                     /locus_tag="ACHY3M_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFS4318273.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13056..13466
                     /gene="menI"
                     /locus_tag="ACHY3M_003429"
     CDS             13056..13466
                     /gene="menI"
                     /locus_tag="ACHY3M_003429"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFS4318274.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13566..13754
                     /gene="ydiH"
                     /locus_tag="ACHY3M_003430"
     CDS             13566..13754
                     /gene="ydiH"
                     /locus_tag="ACHY3M_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFS4318275.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14302..14670
                     /gene="sufA"
                     /locus_tag="ACHY3M_003431"
     CDS             14302..14670
                     /gene="sufA"
                     /locus_tag="ACHY3M_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFS4318276.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14679..16166
                     /gene="sufB"
                     /locus_tag="ACHY3M_003432"
     CDS             14679..16166
                     /gene="sufB"
                     /locus_tag="ACHY3M_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4318277.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16176..16922
                     /gene="sufC"
                     /locus_tag="ACHY3M_003433"
     CDS             16176..16922
                     /gene="sufC"
                     /locus_tag="ACHY3M_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4318278.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16897..18168
                     /gene="sufD"
                     /locus_tag="ACHY3M_003434"
     CDS             16897..18168
                     /gene="sufD"
                     /locus_tag="ACHY3M_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4318279.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18165..19385
                     /gene="sufS"
                     /locus_tag="ACHY3M_003435"
     CDS             18165..19385
                     /gene="sufS"
                     /locus_tag="ACHY3M_003435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFS4318280.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19398..19814
                     /gene="sufE"
                     /locus_tag="ACHY3M_003436"
     CDS             19398..19814
                     /gene="sufE"
                     /locus_tag="ACHY3M_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFS4318281.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19963..20967
                     /gene="ldtE"
                     /locus_tag="ACHY3M_003437"
     CDS             19963..20967
                     /gene="ldtE"
                     /locus_tag="ACHY3M_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFS4318282.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21031..21267)
                     /gene="lpp"
                     /locus_tag="ACHY3M_003438"
     CDS             complement(21031..21267)
                     /gene="lpp"
                     /locus_tag="ACHY3M_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFS4318283.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5570 a         5224 c         5426 g         5224 t
ORIGIN      
        1 atttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc gtatttcccc
       61 tattaactat gttattcaac ggcgtatgac ggaagcaaaa tggtcactca ctaatactga
      121 attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc actttgccaa
      181 actgtttttg cgccatgtcg gttgttcgcc cagcgattac cgtagacaat ttaaaaactg
      241 ttttgcggaa caagaaatcc tatctgaatt tcctcaaccg gtaagtcttg ttggataaat
      301 ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc tgatagtctt
      361 tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg ccgatacgtt
      421 cacaacggac atcacgccag aagcgggaga cgcgcgcttc atcggtatag cccagaccgc
      481 ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcgcaa tacacttttg
      541 ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc actttcagca
      601 ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc tggatcatct
      661 ggttatgacc aatgggctta ccaaaagcga tacgttggtt ggcatagcgg gcggcatctt
      721 caaaggcgca ttcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc tccatctcaa
      781 agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg ctctcttcaa
      841 cttccacatt gtcgagatag acttcgcagg tgctgagcat atgccagccg attttatgca
      901 gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg aaggcttttt
      961 tgggatcttt cggttgcgga tcgcgcgcca gcaccagcat atacgggtac tctttcgcac
     1021 cggtaataaa ggttttttgt ccgttgatgt agaccttgcc gtttttacgc gtgtaagtgg
     1081 tagtggcact gttgttatct gagccagcac ctggttccgt caacgccagg gcataggcgg
     1141 gatcacccgt ttccagggtg ctctctgccg ttttacgtag ctgctctgca gaaccgaaac
     1201 ggcgcatact gtgaatacat tgaccgttgg taattaaaaa tgccggagca ccgcattttg
     1261 acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca ccaaattctt
     1321 ccggcacgcc aagcatggaa ataccgttat ccgccagcgc ccgcataaac tcacgcggat
     1381 atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc gtaatcagtt
     1441 cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc atcattatac
     1501 tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat aaataatctt
     1561 tcgtccatca gtttgagttc tggcgaaatc actggggtga agtccatttt gtcgagaata
     1621 tctttttgta aatcgacgcc aggggcgatt tcgattaaat gcaggccgtc ttctttcagc
     1681 gtgaatacag cacgttcggt gatgtaacga acatccagtc cccgttcgag cgcgattttt
     1741 ccgctaaaag taatttccgg tagttcccga ataaatttat ttacccgtcc ttcctggacg
     1801 atatgcaatt tgccatcggc aatttctgtt tttaaactgc ccgcagttaa cgtgccgcag
     1861 aaaataattt tcttcgaggt ggcactgata tcaataaatc caccggtacc catgattttg
     1921 ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa actcaaataa
     1981 caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc cagaatggcg
     2041 cgggtattca cgttcgcgcc aaaggcgata ccctgtgaag taattccgcc aatcggacct
     2101 gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac caggccaata
     2161 ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc gaataatgca
     2221 cgccgcgcaa ctaatttacg ttggtttagg ggcaggctaa gtttgtgctg tcatcaaggg
     2281 tgaagtcacc agaaataaaa cggttaaccg gcgcaccgcc atacagttgg ctttgatccg
     2341 gatcgaccac cacaatatcc accagataac ccggaatacg gacagattta ggatgcagcg
     2401 tggctttctt aaccattttc tgcacctgca tcatcacaat accgccgtta ttgtgcaccg
     2461 cctgggcaat aaccaatgcg tcgagataca tcacctcatc ttcaaaagtg gcgtagcctt
     2521 cactgtcgca ggtggtagcg cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga
     2581 gatattcttt gttatcaaac tcgaccagtt taatcaggtc ttctttcgtg acttcattca
     2641 gtttgccgcc ttgctggcgt ggatcgacaa atgtcccgat gccaatatca ctaataatac
     2701 caggctggtg ggctgcggcg gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat
     2761 aagcaataat tttattttgt tctgcgagtt cagaaatacg cggcgattgt ccccagtgac
     2821 cacataatgc ccatttcacc agaccttctt gcgccagagg actaatacca cggtcggcgc
     2881 gatcgccaag ccctgtcgga ctaataatcg ataaattacg tggcgtttga gtctgtttat
     2941 atttatcagc aagagctgta attaacgtgg tggcttccag aataccgccg ccagcgccta
     3001 acacacaaag tgttgcttcg tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg
     3061 gcactcgtcc attaatacga ggtggtttta caggtttcat tgttaattcc tgattcagat
     3121 gtgattttcc ctacgccaga tggagaatta gcgcacagag actcacgaaa tatttttatt
     3181 aaatacggtt aaatatattt tttaagttcg gcggggaggg tgttcccgcc gaaatattat
     3241 tgcttatgcc tggtgtaaaa tagttaatac cgtgcgtaaa tcatttaccg agatttgccc
     3301 tggtgcagag gcttttttta ccgcaccaaa agttgccgcc gagccaaata cttcaccagc
     3361 cagacgagaa attacgccag tttttgccat cgacatcgtg atgattggac gatcggcata
     3421 ctgctcctgc atctccaggg tcgcggtaag caacgtcagc acatcgctgg tactttgcgg
     3481 catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat
     3541 gatttcttcg gcttccggcg tttcatggaa gtcatggttg gacatgacaa ctttcacatc
     3601 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
     3661 atcgatcata tcaaccaggc cgctgtcgat ggctgcacga ttgagagcaa tataagcctc
     3721 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
     3781 ttctggcatg gtttcacgga gaatttttgc cgccgccatg acagactcca cattggagag
     3841 gtcggcaaag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
     3901 agcttcggat ttcacgcggg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
     3961 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
     4021 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
     4081 tttgccagtc cataacgtga actgttcagc cccttgccac aacaacatgc cgtatccatc
     4141 aatcgttttg caaccagctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata
     4201 cacgcattca gtgaccagca gtcccggatg taacagactg atatcattaa ctaatgattc
     4261 attctcaagg ggtttcatac ccacttttgt gccattggtt aaaatgtcgg cggaagccag
     4321 ggcttcagca aaggcttgct gatcggcgag atcggtaacc gtgacgacac aatcggtgtt
     4381 ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aactcatccc gacggttaaa
     4441 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaatcgccg ttgaggcacc
     4501 tccggcccct aacagcacca ttgttttgcc tttgatatca aaaccgctct ctttaatggc
     4561 gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg tagccatcat cattaacgat
     4621 ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc
     4681 cagttgtttg ttcggcattg atataccagt tccgcgcatt ttgagggctt ttaatccttc
     4741 aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc
     4801 cgctttttct aaggctttat tctgcatttc aggcgataaa ctgtggcgga taggataggc
     4861 catcagccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
     4921 tgcttaattg tgtttaaccg ggtgctaaat ttacgctcgc caaaccgcac atcattctca
     4981 ggaataataa aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
     5041 agcgcgaaag taaaatcgag aacaatgata tattgaaggc cgatgttcga cagataaccg
     5101 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatataaat actggtgact
     5161 ttggctttgc tttttgggaa aaactctgac atgaccgaaa caccgagctg taaaatgccg
     5221 ccagctgctg agaaaccgat aacaaaggcg ccggcattac acaccagtgg agaagggtac
     5281 aggtagataa tggctgctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
     5341 ggccggacca tttttttcag tagtgcggca aaaataaaga cacagaccaa cgagcccata
     5401 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
     5461 tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt
     5521 accgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
     5581 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
     5641 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatatt attgagcaac
     5701 atatagctca ccagcattgg gtagaacatt tgcccaaatg acaccatcgc tttaaccagt
     5761 atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta
     5821 tccagcgctg agttagcgat acctacgcac actgccagac cgtaggcgag agttaaattc
     5881 gggcaagcgg gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
     5941 gcccgacgac caaacttatc ggagatcaca ccgaagaata aaatactgat caatcgcccc
     6001 aaaccaatac cggaaattaa gtaggcaatg cccgcgttgt cagtggaaaa cttttccgcc
     6061 agagatgaca tgttttgggc aagcgtaata acactaatgc cgtgcaggaa gtagctgaag
     6121 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
     6181 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttcttcga cggtttacat
     6241 aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat gtttccagga
     6301 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
     6361 atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac
     6421 attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga taaacagtgc
     6481 cagtaaaaaa ccgatggcag cgatggcggt atcgaaccac ataatatcgg caatacttct
     6541 ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgcactgta
     6601 atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc
     6661 aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata ttatcaccac
     6721 ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag
     6781 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
     6841 aagcagcgaa cccacggtgt agatactgag tagtttaatc gacatagtgt atgacatgcc
     6901 tgcaacaaat tgttcgtact gtgccagcca ctggctaacc agataaaacg ttgccattga
     6961 gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga
     7021 gctggtggtt ttctttatga caggtaagcg atgacccgga tggggtggga acgtacaacg
     7081 gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa
     7141 ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga
     7201 aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa
     7261 actgggataa gtgcctgcat cgagaaaact gtttgccatt cctgccagaa agccaaaaac
     7321 ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca
     7381 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
     7441 caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac ccgcggcatt
     7501 agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
     7561 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
     7621 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccgttcatta
     7681 ttgttgtaac aacgacaatt cctttttgtg gagaaattaa caaagccgct ccagatcgtg
     7741 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     7801 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     7861 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
     7921 cagttgctcg actacacgat caggaatggc gcatttgcca ttttcccact gacgccagct
     7981 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     8041 atggcgaagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     8101 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     8161 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
     8221 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
     8281 ccatgcttat aaatcaatat gttgatttat aatcgtaagc taatagtagg catacggact
     8341 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
     8401 ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
     8461 cgggggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cggtttctcg
     8521 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg gacttcttcc
     8581 acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
     8641 atcatcccga aagcaatcaa accaccaata acaccagaga ggatcaggat cagcggtaaa
     8701 tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc aaccacaccg
     8761 ctccacacta acaataccgt tccccaggtg gtatcgccag tccagtagag ccagataatc
     8821 gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat cattaacacc
     8881 gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
     8941 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
     9001 agtacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
     9061 accacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag cgcacaatgc
     9121 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
     9181 taagggcgga ctttcgccat gatcgccgtc ccccccatat ccagcaagtt gtgccagcct
     9241 gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
     9301 atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
     9361 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac cagaacggcg
     9421 agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
     9481 accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca cagacatgcc
     9541 acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc cctgggctga
     9601 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
     9661 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggccgatt
     9721 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
     9781 gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt cgttcacccc
     9841 agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct gcaactcgaa
     9901 atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct gaaagaatag
     9961 tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
    10021 caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacaaggt
    10081 tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
    10141 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
    10201 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgtggcggc
    10261 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
    10321 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
    10381 ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
    10441 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcttc tggtcaggga
    10501 tcgctggtct atggcaaaac gtcggatcac gtacttggcg tacgtgcggt gttgttgggg
    10561 ggcgatattc tcgatacgca acctttaccc gtcgaactgg cggaaacgct gggtaaatcc
    10621 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
    10681 ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga tctgcgtcat
    10741 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
    10801 acgctggcct ttattaccga agcgcggctg gatattacgc ccttgcctaa agtgcgccgt
    10861 ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt
    10921 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
    10981 gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa agagatgctc
    11041 gggctgaaca ttgtggagtt tgctggtgat gatgaggcgc tgattgacga gcgagttaat
    11101 gctctctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt gattggctgg
    11161 caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg cgatgcgtaa aaaagccgtt
    11221 ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga ggatacctgc
    11281 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
    11341 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtccagcg
    11401 ctggatatgt gcgatcctca acaagagatt ttgatgaagc aaatctctga tgacgtagtg
    11461 gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg ttttcgcgct
    11521 gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
    11581 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
    11641 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
    11701 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
    11761 tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
    11821 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt tgttggcgga tcgcggcgtt
    11881 gatccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
    11941 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc acacgaagtc
    12001 aaagaggcga tgtcgggctg tctggcctgt aaagcgtgtt cgacccagtg ccccatcaaa
    12061 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
    12121 ccgctgcgcg accacctcgt cgctacggtc gagagctacg cgccgctgat ggcacgcgcg
    12181 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
    12241 atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg
    12301 catcgctcgg caaacatgac gctggaacag cttgaatctc tcaatgcaga gcagaaagcg
    12361 cgcacagtgt tggtggtgca ggaccccttt accagctatt acgatgcgca agtggtggcg
    12421 gattttgtcc gtctggtcga aaaattaggt ttccagcctg tgttactgcc attttcgcca
    12481 aatggcaaag cccagcatat taaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag
    12541 acggcggatt tcctcaaccg tatggcgaag ctgggtatgc caatggtggg cgtcgatccg
    12601 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
    12661 tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgagtcaca gccagtggct
    12721 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac cgccttgccg
    12781 ggtgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
    12841 agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccataaaaat
    12901 tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc gcgtaaccgc
    12961 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg cacgggggta
    13021 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg aaaatcaccc
    13081 tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat attcgctttg
    13141 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
    13201 ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc ggttccgttg
    13261 ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc
    13321 acgtccgctc ggcacgagaa gggcgggtgc gcggggtatg caaaccgttg catctcggtt
    13381 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    13441 cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgcct
    13501 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    13561 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
    13621 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
    13681 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
    13741 tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct ggattaacct
    13801 ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat tgttcaaaaa
    13861 taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt attcttatcg
    13921 ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg ggtagaaacc
    13981 cttaagcctg tgttgcacag acttaagggt tttcttattt ctgaacgctg cactttttta
    14041 cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca gccttaatgc
    14101 tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt gcctgttaac
    14161 ccacacatca gggtctatgc ttattaaaag caggcaaaaa gcatgtttta cggtaaagcc
    14221 cctgcgtttg ctgggttgta ctgataatca ttatcactaa catgctgtta tacgctgaaa
    14281 gcgatgaagt gaggtaaatc gatggacatg cattcaggaa cctttaaccc acaagatttc
    14341 gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgtga gctggtggca
    14401 aagcagccgg gtatggtcgg cgtgcgctta ggcgtgaagc aaacgggctg cgcgggcttt
    14461 ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt tgaacacgac
    14521 ggcgcgaagc tgtttgtccc gctgcaagcg atgccgttta ttgatggcac ggaagtcgat
    14581 ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc ccagaatgaa
    14641 tgtggctgtg gcgaaagctt tggggtatag gcggtactat gtctcgtaat actgaagcaa
    14701 ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga ttcttcaccc
    14761 agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg
    14821 cgaagcgtaa tgagccggag tggatgctga agtttcgtct aaacgcctat cgcgcatggc
    14881 tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat tatcaggatt
    14941 acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg tctgaacctg
    15001 gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg
    15061 agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc attttcgact
    15121 cagtttcggt tgccactact tatcgcgaaa aactggcgga gcagggaatt attttctgtt
    15181 cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc ggcaccgtgg
    15241 tgccggggaa tgacaacttc tttgccgcgc ttaatgcggc ggtagcctct gacggtacgt
    15301 ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat tttcgcatta
    15361 acgcggaaaa aaccgggcag tttgagcgca ccattctggt ggccgacgaa gacagctacg
    15421 tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagtta cacgcggcag
    15481 tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta caaaactggt
    15541 tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg
    15601 aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtccgcg attacgtgga
    15661 aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac tcagtggcgc
    15721 tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc ggtaaaaaca
    15781 ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac agttatcgcg
    15841 gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag tgcgactcaa
    15901 tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca
    15961 gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa ctgttttact
    16021 gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac ggtttctgca
    16081 aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca
    16141 tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa gatttacacg
    16201 tcagcgtgga agataaagct atcctgcgtg gattaagcct cgccgttcgt cccggcgaag
    16261 ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca acgcttgccg
    16321 ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa gatttgcttg
    16381 cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag tatccggtgg
    16441 agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca gtgcgcagct
    16501 atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa gagaaaatcg
    16561 ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt ttttccggcg
    16621 gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag ttatgcattc
    16681 ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc gatggcgtga
    16741 actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa cgcattctcg
    16801 actacatcaa gcctgattac gtccatgtgc tatatcaggg acgaattgtg aaatccggcg
    16861 atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc gaacagcagt
    16921 aacgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa
    16981 gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa acatgaaaac
    17041 tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat tgcgggagag
    17101 atatccccac agcagcgaga tgccttagcg ttaacgttag acgctgtgcg gctggtattt
    17161 gtcgatggac gttacgtgtc agcactgagc gatgcgactg aaggcagcgg gtatgaagtg
    17221 agcattaacg acgaccgtca gggggtaccc gacgctattc aggcggaagt gtttctgcat
    17281 ttgacggaaa gcttggcaca aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg
    17341 gcaaagccat tgctgttaat gcatattacc cagggcgtgg caggtgaaga ggtcaacact
    17401 gcccattacc gacatcatct ggatctggcg gaaggtgccg aagcaacggt gatcgaacat
    17461 tttgtcagcc ttaatgatgc tcgtcacttc accggcgcac ggttcactat caatgtcgca
    17521 gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag tcaccacttt
    17581 gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag tttcctgctg
    17641 ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa cagcacgctg
    17701 cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg tacctggctg
    17761 gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt cagcgacaaa
    17821 ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa aacggatggt
    17881 cagatgacca acaataatct gctgatgggc aaactggcag aagtggatac gaaaccgcag
    17941 ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg gcgtattgat
    18001 gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg
    18061 atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg gcttaaacag
    18121 caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact ttttccgtcg
    18181 acaaagtgcg ggccgacttt ccggtgcttt ctcgtgaggt aaacggtttg ccgctggctt
    18241 atctcgacag cgccgccagt gcgcagaaac cgagccaggt gattgacgcc gaggccgagt
    18301 tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc gcccaagcga
    18361 ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg
    18421 aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc aatagctggg
    18481 gtaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag caccacgcta
    18541 acattgttcc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt gtgatcccgc
    18601 tcaatcccga cggtacgttg caactggaga cgctgcctac gctgtttgat gagaaaactc
    18661 gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca ctggcggaaa
    18721 tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct caggcggtga
    18781 tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc tccgggcata
    18841 aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg ttgcaggaga
    18901 tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt gaaggcacaa
    18961 cctggaccaa agcaccttgg cggtttgaag ccggtacacc caataccggg ggcatcattg
    19021 gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taataacata gccgagtatg
    19081 aacagaatct gatgcactac gcgctatcac agctggaatc tgtaccggat ctcactctct
    19141 atggtccgca gaacaggctt ggcgttattg cttttaatct tggtaaacac cacgcctatg
    19201 atgttggcag ttttctcgat aactacggca ttgctgtgcg taccggacat cactgcgcta
    19261 tgccattaat ggcctattac aacgtccctg ctatgtgtcg ggcgtcgctg gccatgtata
    19321 acacccatga agaagtggat cgtctggtga ccggactgca acgtattcac cgtctgctgg
    19381 gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg cgtaattttt
    19441 tacgctgcgc caattgggaa gagaaatatc tctacattat tgagctgggc cagcgtctgc
    19501 cagaattacg cgacgaagac agaagcccac aaaatagcat tcagggctgc cagagtcagg
    19561 tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc gacagcgatg
    19621 cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag atgacgccgc
    19681 aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc
    19741 tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc gccaaagccg
    19801 ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt gccggatggt
    19861 caatgtttac actgacctgc acaggagtgt ttcggtttca ggtgaacata agcgccatcc
    19921 gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg cgcgtctttg
    19981 cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc ggttgattat
    20041 ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac ggtgcaggaa
    20101 ggggataaaa accttcaggc catcgcccga cgttttgata ctgcggcaat gttgatcctt
    20161 gaagccaata acactatcgc cccggtgcca aaacccggta cgacgataac tattccttcg
    20221 caactgttat tacctgatgc gccgcgtcag gggattatcg ttaaccttgc agagctgcgc
    20281 ctttattatt atccgccggg agaaaatatc gtgcaggttt atccgatagg tattggcttg
    20341 caggggctgg aaacgccggt gatggaaacg cgtgtggggc agaaaatccc taacccaacc
    20401 tggacgccta ctgcaggcat tcgtcaacgt tcgctggagc gtggtattaa attaccgcca
    20461 gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct cgcgcatggt
    20521 aatggcgaat acctcattca tggcaccagt gcgccggaca gcgtcggttt gcgcgtcagt
    20581 tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag cgtgcggacg
    20641 ggaacgccgg tgaaagtgat caacgaacct gtgaaatatt ccgtggagcc taacgggatg
    20701 cgttatgttg aagtacatcg accactatcg gcagaagaac agcagaacgt tcagacaatg
    20761 ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt agatcagaag
    20821 ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag cagtggagca
    20881 actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga accagagcaa
    20941 gggaatatgt tacgcgcgac gcagtagcgg taaacggcag acaaaaaaaa tggcgcacaa
    21001 tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc
    21061 cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc
    21121 gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag
    21181 ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc
    21241 gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt taatctccat
    21301 gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt gatgggaagg
    21361 cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag ataaaaaaat
    21421 tgagagaaaa caggatgctt ccat
//
LOCUS       DAZUFB010000083        21270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZUFB010000083 DAZUFB010000000
VERSION     DAZUFB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.083"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..793
                     /locus_tag="ACHY3M_003439"
     CDS             <1..793
                     /locus_tag="ACHY3M_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFS4318284.1"
                     /translation="PASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVP
                     RISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNG
                     NMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGP
                     VSPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLLVRS
                     RAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            797..2128
                     /gene="traO"
                     /locus_tag="ACHY3M_003440"
     CDS             797..2128
                     /gene="traO"
                     /locus_tag="ACHY3M_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFS4318285.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQSRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2125..2838
                     /gene="traP"
                     /locus_tag="ACHY3M_003441"
     CDS             2125..2838
                     /gene="traP"
                     /locus_tag="ACHY3M_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFS4318286.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVAPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMKIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2835..3365
                     /gene="traQ"
                     /locus_tag="ACHY3M_003442"
     CDS             2835..3365
                     /gene="traQ"
                     /locus_tag="ACHY3M_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFS4318287.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3412..3810
                     /locus_tag="ACHY3M_003443"
     CDS             3412..3810
                     /locus_tag="ACHY3M_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFS4318288.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            3867..4118
                     /locus_tag="ACHY3M_003444"
     CDS             3867..4118
                     /locus_tag="ACHY3M_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFS4318289.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4033..4851
                     /locus_tag="ACHY3M_003445"
     CDS             4033..4851
                     /locus_tag="ACHY3M_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFS4318290.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            4910..5269
                     /locus_tag="ACHY3M_003446"
     CDS             4910..5269
                     /locus_tag="ACHY3M_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318291.1"
                     /translation="MDTHSAGYQFTADSFRRFIIRTLTDNIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHKSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5269..5649
                     /locus_tag="ACHY3M_003447"
     CDS             5269..5649
                     /locus_tag="ACHY3M_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318292.1"
                     /translation="MKIHRISPETLITLIHAHLAGKTDSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(5622..6167)
                     /locus_tag="ACHY3M_003448"
     CDS             complement(5622..6167)
                     /locus_tag="ACHY3M_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318293.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            6392..9436
                     /locus_tag="ACHY3M_003449"
     CDS             6392..9436
                     /locus_tag="ACHY3M_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4318294.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPHAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            9436..10053
                     /locus_tag="ACHY3M_003450"
     CDS             9436..10053
                     /locus_tag="ACHY3M_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFS4318295.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLPLPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            10014..11219
                     /gene="traW"
                     /locus_tag="ACHY3M_003451"
     CDS             10014..11219
                     /gene="traW"
                     /locus_tag="ACHY3M_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFS4318296.1"
                     /translation="MQRQPATTLLALLIGCTVIPSTHAYPVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            11216..11785
                     /gene="traX"
                     /locus_tag="ACHY3M_003452"
     CDS             11216..11785
                     /gene="traX"
                     /locus_tag="ACHY3M_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFS4318297.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11858..14023
                     /locus_tag="ACHY3M_003453"
     CDS             11858..14023
                     /locus_tag="ACHY3M_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFS4318298.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAK
                     AFLEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALI
                     NVQINSVTGVFSMAGFLMIYARACTTLVAGVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            14094..14747
                     /gene="excA"
                     /locus_tag="ACHY3M_003454"
     CDS             14094..14747
                     /gene="excA"
                     /locus_tag="ACHY3M_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFS4318299.1"
                     /translation="MNTTKNRYPSWWGYYYLIRNTYFIFGIPFLLLCSLGFTGMMLTE
                     PYIGTDIQAYLYTCGSWFLLIIPSLWLYLRTKAKKKKILQVVKKIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            15021..16607
                     /locus_tag="ACHY3M_003455"
     CDS             15021..16607
                     /locus_tag="ACHY3M_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFS4318300.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFTAGIPKARRDEIKNSLSLISQLQKQRLR
                     ERRAELAHARAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(16745..16897)
                     /locus_tag="ACHY3M_003456"
     CDS             complement(16745..16897)
                     /locus_tag="ACHY3M_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4318301.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMLRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            17230..17472
                     /locus_tag="ACHY3M_003457"
     CDS             17230..17472
                     /locus_tag="ACHY3M_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318302.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(17565..19100)
                     /locus_tag="ACHY3M_003458"
     CDS             complement(17565..19100)
                     /locus_tag="ACHY3M_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4318303.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSACYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(19300..19755)
                     /locus_tag="ACHY3M_003459"
     CDS             complement(19300..19755)
                     /locus_tag="ACHY3M_003459"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFS4318304.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLGENSQVTEYFIISIATFLFLSGLCLLFIYSFFKRKRVVGKLLLAIHLLYISHYA
                     ITFVYCLYVVGAECMTTGKIEYILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(19843..20466)
                     /locus_tag="ACHY3M_003460"
     CDS             complement(19843..20466)
                     /locus_tag="ACHY3M_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4318305.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKVPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            20625..21128
                     /locus_tag="ACHY3M_003461"
     CDS             20625..21128
                     /locus_tag="ACHY3M_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFS4318306.1"
                     /translation="MIKYIFALTLLLSGSVCAVQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVANTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNDHTEL
                     QINNILP"
BASE COUNT         5616 a         5724 c         5063 g         4867 t
ORIGIN      
        1 acctgcctct ccccttgttc aggaagccgc atcactggat tcccccctga atgcaggtga
       61 aattcgggaa ctgcggggac gaatggccga taacgaacgg gccatcagcg cacctgtcac
      121 cagtgtggtg ccccgtatca gctccctcac agtaaatctg tcacccgggg ccagtctgcc
      181 gcttgtccga accgcactgc ataacgtcag cgtggtaacg ttcacggaca tcaacggctc
      241 cccgtggcct cagtcggatc cgccttataa cgcggcaccc ggtcactttg atgtgcagtt
      301 caacggcaat atggtctccg tcactcccct gcgcgcctgg gctgcaggga atgtaacggt
      361 ctacctcaaa ggactgtctg taccggtcat tctgaatatc accagtggtg aaaccgacac
      421 caataccctg agtcaggaga tcgacagccg tctggatctg cgcattcccc gtcagggacc
      481 tgtcagtcct gtggtagccg taccatcaga gaagattgcc ctgcatgatg ccaccctgca
      541 ggcctttctg gacggtgttc ctcccgatga tcccaccgtg cgtcggctga aattctccgg
      601 aaatgttccg gacaccacca tctggcagca cggcgatgat ctgctcgtgc gatcgcgggc
      661 catgctgcgg gatgaatttg aacagacgct ggcatctgct gacggtacgc gtctgtggaa
      721 actgccggtg actcccttac tcaccttctc cgtgaatggt cagtccattc acgtcacccc
      781 tgaactggag taaatcatga gcgcagaaca ggacacagcc gcaaagtccg gcaaaaaact
      841 gtcagccctg ctggttctcg ggggcgtgat tatcatcggg ggcggatacc tgctgctcac
      901 ctggctttcc cccgaaaaca gcgcatcgct ctcgtcagtc aatatcaaca gcacgacaac
      961 aggcagcggg cggaccgtgg tggagtcaca gcactaccgg gatttactgc gtgctgacaa
     1021 tgagcgcggg gccgcagaag cattacgtaa taaccagacg ttcatcgcca gtctgcccag
     1081 gggactggat actgatcacg gaaagaatca aagtagggat actcagcctg ctgaccaggc
     1141 cgagggttct ggtcaaccat caacgccccc tcaggcagat caaacagccc gggaaaaaca
     1201 gcgggaagcg ttacaaaaac tgcttgcccg catcaacaca cagcatcctg acgcccgccc
     1261 cccggtagtg gcggctgcaa tgtggggtac cactggtgct cccgggataa gctcacacgc
     1321 ctcaccaccc ggtaccggca catcaccaca accacgtctg caggaggcca gtctggcaac
     1381 cccggcagct cgtacaggga tacagctgat accggcactg acccgcgtac ctgccttcat
     1441 tgatacggaa gtggactctg acaatgctgt ctcccgcgtg gtggcctccg tacctgccgg
     1501 cccatgggcc ggggcagtcc tctacagcca ggacgccaga ctcgccggca agggaatgga
     1561 tatccacttt gaccggatga tgtggcgggg catgtttctg aaagtcaatg catatgccct
     1621 gaatgaacat accggcatgt catccgtggc cagcgacgtg aaccaccgct ggtttaagca
     1681 tatcgttctg ccgtctgtgc tcggtggggt gggcagtgtc ggaagtcttt acaaagacgc
     1741 caacacacag gtcattcagg gaaactacgg cagcattacc ggcagggtgg gaatgcctga
     1801 tggcgaggcg ctggctggcg tcattgcagg cggcatggcc gaacggggca gtcagttgct
     1861 gacccgccag gcggagagcg aaccttaccg gcaggtgaca gtgtcacgcg gagaggttgt
     1921 ctccattctg tttgttgagc cggtcatgag caacgatatc agtcccccct cctccggcac
     1981 tgccccttct gtgccaccgc cctccggacg cccgctcacc caggaagaag cagagcgtca
     2041 ggcgtctgac aggatccagg cagctgtcat gcgtaaacag aacgaaatgc ggcaacgtta
     2101 cggaatacaa caggaaagtc atccatgaaa aacgaacatg aagatcccgg cagttttggc
     2161 gaaacaacga caccggcatc cggcaaatcc ttcctgaaac gccagtatat gggatacagc
     2221 ctgccagtct gggccggctg tgtcctcatc atcctggccg cagtctggta caagtttctg
     2281 cggacaccct cagagccccc tgcagatgca ggcttcgcgg tcacaccgcc cgcagccgaa
     2341 agctgggcag agcccatgcc catgacggag accgggagcg gaaacactat gtccgcacct
     2401 cctgtcgccc cacaggcaga catgcagcag cttgcccgtg acctgaaagc agagctggac
     2461 agacgggatg aggaaatccg acgtaccctg accatgctga aagagagcat cacaaccctt
     2521 ggcgacacca tacggaaaga tgaggcctgg gcacaggaaa cccgtcagtt gcttgatgaa
     2581 acccgtaagc aggtgcatgc cctgcagatg cagggaccac tgcaaatccc gaccgggaat
     2641 aacggccagt caacgaaaac cccgccttcc cgcctgcagg gcatgaaaat catgtcggtg
     2701 gaaaaaggaa tggcctggat ccgctggcag ggtagcgcct gggcagtacg ggaaggtgac
     2761 aggctgggga atatcaccat cacccggatt gaaccgtcca gccgctcagt catcacctct
     2821 gccggtacat tacgatgatg agcattgatg ccctgacagc catagagaac tttgccagcg
     2881 gtatattcag tgcaggcatg gagtttctct tcacctgggg agagctgctg gggattatcg
     2941 gcctgattgg tcatctgatg cgtgcccggg ctgaagggcg tcacagtatg ggaccgggaa
     3001 aatttatcgc cggtatcgtc atctgcggca tgctggtcgc cctgccatcc ttcatcaatg
     3061 ccgggggtac ccagatggga ttcagggcag attccttcgg ccctatcgct tatgtacaac
     3121 caaccacctt tggtgccgct gccggtgccg ccaatgccat gctctcactg gtaaaactgg
     3181 ccggagtggg gtttgccatg aacggcattt ccatctggcg taaatccctg ctggacgggc
     3241 ataccgccct gagcagcagt gagtccatca gcaaaggaaa cgtcaagttt gttgccggcg
     3301 ttctgctggt ttttatcgat cgcgtcatcg atgcctctct ggcgagtctt ggtctcgcct
     3361 tctgatccac atcctttttc agtaattcag tctgttagtt aaggagacta tatgtcacga
     3421 ttccgtgcca tggcggcgcg tctgtgcgct gcatttattt atgttcagtt atgcctggtg
     3481 gcctgccgtg atcggctgat acgcctgtta cttctgcttc ccgtgatact gctgccccac
     3541 acagcgatgg caaacaccct attcgatatg atcaaaaccg ttggcgatgg tgcaaaaggc
     3601 tccagcagtt catttgtggc tatcggtcag gttattggcc tggtgctctt tatcggcggc
     3661 ctgcttggcc tcaaacaaat cggtaaaaaa cccggtgccg ggctggcctg cctgctgtca
     3721 attctggtcg gcctctgcct tgccgtcgta ccggaactga tgggtagcgg acagaaaacg
     3781 ctcggtcttt ctccggtttc aatcagttaa tccgcaaagg caccgggaaa ccggtgccgt
     3841 cttcacacaa tgaggaggac cagaccatgt cctgtgagct gccccctttc accctgctca
     3901 gaacagagca cggcaacgtg attgcccgcg acaaccggga taatcaggag tttgtgtttg
     3961 cagaatgtta ttccaccgcc atcgcagcac tgattctggc tgccgtcacc ctgtatacag
     4021 agagaaaaac gtatgaacaa aacaccgcca ctcatcgccc cggtccggga acgctggctt
     4081 ttcccgctcc cgcaaccgga cacggtattc agcggtgaat ccctgctgcc accaccagtg
     4141 ctctcaacac ccggacgctg tccctgctgt cgcaggaccg tcactcaccg cttcattctg
     4201 gaggattcat ggccattaca gcagatggct gacacctgcc gggatacggt cgtcctgctg
     4261 gaaaaaaacc tcacccgggt gatgcgcctg aaaaaacatc cggtaccgga aaatgctgac
     4321 gaaaaaaaga aacacacccg aaccctgcag gatgcggagc ggtccctggc acaggcccgc
     4381 ctgtcggcca ggcgactggc cctgcgtcat gtggaaaagt cacaaatcgt cacaacagat
     4441 gcactcagcg aaaatgaaag tgaactgctg cagccggaag gcccgccctt tcatctctgt
     4501 gccttctgcc acgcctggca ctgcctgaac ggctacgctg ccgcacaggg ggtgatggtc
     4561 tggctccctg atttgcatcc ggcctcggta gttgctctca atgcacgggc actgaaggaa
     4621 attttcagcg atgagcgtaa gcgagtcaga cagggacggg ccgtactgaa tgccctggta
     4681 cagaaccgcc tggccgttga ggagaaattt cgcacctggc gtccggcgga ttttgctgat
     4741 gcactgcgcc gctggccgcc ggcacagcgt aaaacgttga gggagaaaat ggacggggtg
     4801 gcgctcattc tgctgccgga ttcgtttcct gacaaaaagt acgtcatgtg acacacaccg
     4861 gtaccgatga cattctgccg gcacacattc aacttacaga ggaacaaaaa tggatacaca
     4921 ctctgccgga tatcagttca ctgcagattc attccggcgt tttattatac ggacgctgac
     4981 tgacaatatt ccgcctgacg gagatgaaaa agaaattgcc cggttcattc tccgccaccc
     5041 ggcatttaac ggcggctggc tgctctttct ggctgcagaa attcatgaaa gatcccgtac
     5101 ccataaaagc atcaccgtca cagtgagcgt cgataactat ctctcatgga tggatattgc
     5161 cgtatccggt ctcccggaat taccggatat gcccttctgt gattttttca ttcgccgcca
     5221 gcagtttatc tatccgggtg tgcccccgac aaaatcagga gaagaatgat gaaaattcac
     5281 cgcatatccc cggagacgct tataacactc atccacgcac acctggccgg aaaaactgac
     5341 agtaccgcca aagaagagca ccggctactc cgtcgtttcc tgcgggatga tgacggcagg
     5401 ctggccggcg tactgctgaa tattgccgga atacttcagt tcaacaggga attaagtgcg
     5461 cggcataatt atccggccac acccctgact gaattttcat taaggaaacg gggaaaacaa
     5521 cttcatctct gtctctgcag tctccgtttc ttttatattc ctccggtatt tatacaaaac
     5581 aaaagaagaa aatctattgt tgtacatctt aataagatca cttataaaca aacccattca
     5641 atacgctgac gaagtgattc atactgatta tactcatctt tgcctatatg ttttttaagc
     5701 gtattaacaa tatcatattg aggatgtcct gaattgtcag tatacagtgt tgttgcagga
     5761 aggactgcat aatctgatat ttcacaacct gtatacagtt ctttgtcagt tcggcaggaa
     5821 tggcaaagag aaagacatgt atacaggtct gtgttttctg cctcctcagg agataaccac
     5881 gacacatatt gcaaatcatc tgtatcagga taacaatttt cttcctgaac ctgaactctg
     5941 aaaatataga aaaataattc atcttttgaa aggcatgcta ttttattccc ccaatgcacc
     6001 gcattatcaa gttgatccga taaataaatc catcctggtg tcgtctctaa cgaccagcat
     6061 ataacagaag gagcatcagt tcttattttc ccatctttta ttatttgcag acctctgctc
     6121 attgttgtcg catgaaaaag atttaatatt ttattttcag ccttcactct atttcctcat
     6181 ttcccaagaa acatattaaa aacggaaaac attacacata acctggagga caaatcatga
     6241 ctttaaattt ccctgccggg gaaactacac tattctcagc aaaaaacatc ctgacagcaa
     6301 tactcactct gcacaactta cacgcatagc gttcccattt gaatacagtt gcagcccgcg
     6361 atttattttg tcttccggag ccacctctgc catgaatatc aaccgcatta tttttgccat
     6421 tgaagactgt atcagtaccc tgtcacggta ttccgtttcc gcattcttcc ccgattactg
     6481 tgatctgcac accgtcatcg gtctggacca gcaggaccgc gaacgccgcc cctggctgaa
     6541 agccccctac attgccacca caaaacaggg gtattacctt tccgtctttg aaatcagcgg
     6601 tgccctgaaa gaaatggatg aaaacccgga cctgacagcc cccggcacac tggaatcact
     6661 gattacagcg ctggcagagc gaatgaacac tgcctataaa aacaccggtc acaaaatcag
     6721 ttttgttttc gaacgcgatc cggagcgtgg aagggatgaa atcgaagcca tgctcttacc
     6781 ccagcgaaaa tccatcgcac gcacggggat cctgcttcag gacgttctgg atgaaaaagt
     6841 caccacgctc accccctggc ttgtccggga acgctgctgg ctgaccgtct ggagctctga
     6901 agaactgctg agccggaccg atcgcaagga ccaccagacc cgtatcagga aactcgctga
     6961 acacgctccg ccagcccgat ttgcgcagga tccatggcgc tggacacttt ccgcactcaa
     7021 aatacggcat gacgcctttc tggacacgct ggagcaggcc ctgacacatg attctgatgg
     7081 tctgctggtt cgcctgatgg atattcatga agtgggacgg gaaatccgcc gtcaactgga
     7141 gcgcaacagc acacctgcag tatggcagcc tcatctgcct gaagatgccc gtcctgccgg
     7201 ctggcgacag ggcgaggata cctctgtatt ccacgcaccg tcgctgaatc tgcagttatt
     7261 caccacacag cctgaaacac acggcagcct gataaaggcc ggtgacctct ggcacggtat
     7321 ggtcgccatt accctgccac cccagaatct gcggacattc aaccagctgg tcagggatgt
     7381 gccccacgca atcccctggc gtatccggat ggatctgatg cccggcggca tgaaggcgct
     7441 tggggtcaag aaaaccctgc tgacctacag cagctttatt tcccctctcc ggcccatgta
     7501 tgactcggtg atgatgctga atgacatcaa caaacatgat ccggtctgta tcatgactat
     7561 tgttgccacc acatggggaa acagccggga agtctgtacc cgcaaccagg ctctcctgaa
     7621 atctgcgctg gaaggctggg gagtatgcgg caccaccacc accttcggtg atccgagacg
     7681 tgcctgggtg aacacggttc tggcggcctc ccacggttcc ggtccagtcc ccctttaccc
     7741 gcccctttca cacgcccttt ccctgtttcc cctgaacaga gccggctctg tctggcgggg
     7801 acagggaaat ctgatgatgc acaccgaaga cggctctgca tgggaggtcg cgctggcatc
     7861 gtcacaacag aacaagcaca cggaactgac gcccggcgca cccggtctgg gcaaatcggt
     7921 actcatcaac gcactgtcgg agatccagat agcctcagcc cagaagaacc tgcctttcat
     7981 tgcctatatc gacaagggat tcagtgcaca gggactggta cagcttatcc gtgacagcct
     8041 gccggagcag agaaaggacg aagccgtggg gatcattctg agcaatgatc cggaacacac
     8101 ccgtaacctg tttgatgtga tgtacggtgc taaaaaaccc gccaccccgg aaaagaactt
     8161 catggtatct gtactctgcg cactgtgcgt ggataccggc accggtcagc cctgtaatcc
     8221 cggtgacacc cggcaaatca taagcaacct ggtggatctg gccttcaggg agtatggcga
     8281 aaacaacccc agactgtacc gtgcaggaac agagcccctt gtggatctcg ccctggaaga
     8341 gagtggcatc gcagaacaac atgacgctgg atggtggaat gctgccacct ggtttgaaat
     8401 cagggacatg ctgcatatgg ctggcaatat ccccgcagcc cagcgggcac actaccaggc
     8461 catgcccctg ctggctgaaa tgtccgccct gctgggacag ccatccatcc gggatgtgtt
     8521 cggcacagtt cagcgcgata actcagcaga attgttactg gactacatcc gccgtgccct
     8581 cgatcaggga cacagtgact acccgatgat gtccggctgt acccggttta tgctcagccc
     8641 cgacacccgc gttgtggccg ttgatctgaa caatgtggcc ggtgacaaga cacctgccgg
     8701 gcgtctgcgc accggcatca tgtacctgct ggcaggacaa atcgccggcg gggattttgt
     8761 cctgccgcag tatcaggagg aaatacggaa aaatctcgac ccacgctacc atgaggtgat
     8821 tttccgccgc attgaacagc ttgatcagga ggtcaagacc aaagtctatg acgaactgca
     8881 caatgcaaaa gggatcaatt tcatctggga agccctggac acccaggagc tggagcagcg
     8941 taagttcggg atacgcacag tgctgagcac ccagctattg caggactatc caccggcagt
     9001 tctcaaatcc gccaacaccc tctggttgct gcgttaccgc cctgacgaaa ttccgtttct
     9061 gcgggataat tttggtgtcc ctgaagtcac cctgcggcgc ttcctgaaaa tgccggaagg
     9121 ggctgcaccg gatggcagcg gtgtcccggt actggctgtg ttccgtgtaa aaaacggtac
     9181 gctggcccgg atcctgaaat tcactctcgg tccactggag ctgtgggcac tgaattcctc
     9241 acccaaagac agcgccctga gacgtgcgct gacgcaggag ctcgggtcac tgcgggcacg
     9301 ccagatcctg gcagaaaact ttccccgtgg ttcagccacc tcactgatag aacaccgcgc
     9361 ccgtacacat gacagtgaaa acgtcatcca tgatctggct gcagaactca tccgtaagca
     9421 gggatatcac ctctgatgta cagcatacac atcactcccc gctgccggcg tcctgcacgg
     9481 ggacaggtct tcctgaaaac gctgcgcgtg gacggtgaca gtttcagctt caccctgccg
     9541 tccgcctgct tcacgctgcg tccctccccc aatccccggg tcattttcgc cagtgacaga
     9601 cgggctgacg gggaaatgag tggcgttcgc ctgcctctgc ccggagagac aggcacgctg
     9661 ataacaacgg ccacctggca ggtcaacgtg ccggagcggc ctccttttga tatacagcat
     9721 gtcattcact ggctcctgcc ctcgcctctc acggcgctga tgtccgacaa cacctggctg
     9781 tggccggata gccccggcac accggcactg acggcgggag actggccggt aatggaaacc
     9841 gataccccga cgctgaaacg cgtgaagcgt cgtaccttca cgctcatcga taaccgcaac
     9901 cgtcagggat ggatcacccg tcgcttccag tgcttcccgc tggctccgct gccggaatca
     9961 cacagtcacc gcgtaccaga catcctgtat cccctgaaaa cggagaaaaa agaatgcaaa
    10021 gacaaccagc aaccacactg cttgcactcc tgataggctg tacggttatc ccgtccaccc
    10081 acgcctatcc tgtgaccgta gtggccagcc agcctgtcac cacacaaatc atgccggagc
    10141 tggccgccat tcagggcgtg atggcagaga tggtccgtat gcagacagcc accggcacag
    10201 ccatcaacca gagttcagag aagctggcct ctgtcatcgc acaggatggg caggccaccc
    10261 gtcagcagat gatattcagt aatgaaaccc gccggcttga ggaagcccgg aagagcttca
    10321 gcgtgccgga ctccatctgc agtgaatccg cctcaggagt tgcagctgaa agtcgccgtg
    10381 cagcagcgtc tgcggctgca cgtctgagtc agggaggagg cgtcagcagt aaaccaatcc
    10441 gtgaacgtct ctcccgggca gcagactcac caacccgcga ggcatatgac agtgcaggca
    10501 ttcatgccgg ttactgcacg gaagctgaat atgcccgctt tggcggaacc gatgtctgcc
    10561 ctgccgtggg cgagctgccg ggcggagaca gccaggtgcg atcgctctat cagggtgcag
    10621 gcacagcaga tacccctgcc gccctcacct gggatcagaa acagattgat gctgccacgg
    10681 cttacatgaa aaacaccgcc agaccgtcag cgggtcgtgc cccggggaaa ggtgaagtgg
    10741 gaacacagac cgggcgcacg tatgtgggcc tgcagaatga atacaacggc atcatcgatg
    10801 ccgcttctca tccgcaactg tcactgatag ctgacagtac ccctaatgag gccacccgtg
    10861 gagcgctgac tgaagccctg cagtcaccat ccgctgcggc atattttgac cggaccgcat
    10921 catcagaggc ccgcacccgt ggtcacatga gccagcggga gtttgaggcc tttgaagctg
    10981 gcagacgcta tgcaaacacc gactggcagc aggatctgca gggcatggaa ggcgataacc
    11041 ttctccgtga actgctgcgc actaccgccc tgctgaactg gcagatgaac gacctgaaag
    11101 agcagatacg ccagggtaat gttatcgccg ggcagcagct ggcactggcg gcacgacagt
    11161 attacggaca gcgtctcggt gaactgtcac aggccatgag ccaggggagc gtaagatgaa
    11221 agacgacaac agcaccgaca aaaaaaaacc ggccccccgt ctccgccgca tcggtgacat
    11281 cgggataaac ctgttcgcac cactggcaga aacccggtgg atgctgcgca gctgccgcca
    11341 tatgctgggc cagcataaaa cgcgcctgaa gcgcctcatg cccgaccggc aaccggagag
    11401 tccggtctgt atgacctggg aagaagccgt cagggccagc ggccttacgg tcaacgaact
    11461 ggatcggcgt ctgcgacgtc gcaggatgtt ctggcggaca ggattctggc tgatgatgat
    11521 accggtactt ctgctgtgcg gcatggccct tgctgccaca gcgcttccgg ccatcaccct
    11581 gatacgtata ctggcctgcc tgttactgat gagtgccatt gcagcactgt gtgcctcaaa
    11641 aaccctgatt gtgtcatacc gcctctggca actgaatgaa cggcgggtca gcgaggacga
    11701 acagggcacc ttccccgatt acctgcaggc ccggcacggc tggaaacagg ccacatggct
    11761 ggccattacc caccggcagt actgaccgtc cggtctgcaa ttccttttcc tcattccgga
    11821 ggctgatgcc ctgcatccct tcggttccgg agttatcgtg aaaatcatac ccagagcact
    11881 gagtgcgggt ctggcgctgg ctgccgtacc tgcacaggca ttcgtcacct atcaggacat
    11941 tgtgagcgcc gccaccaatc cggatgacct gtcgcgccag gcactggtca ccatttttgg
    12001 cgacgtggtc acaaatcccc tgacagccag cagcaacacc attatcggta acctgttcat
    12061 ggtgtttaac gcaattatcg ccattatggg gatagtctgg ttcctgttta ttggaatccg
    12121 ccatgttgtc cgcaccggcc accagggcaa tgtctttaac aacggacggg acgtcgtcgg
    12181 aatgctcagt attgtgagcg gttttatgat gattgtgccc accgcttccg gctggagcct
    12241 ggcacaactg gtcatgctct ggggcgcatc catcatgggc attggttccg ccaatatcat
    12301 ggttcagacg gctgcagaaa acatcgctga aggctattca atgaccgtac agccagttca
    12361 tgtctccacc cgcacggcag cacggggaat actggagatg gcgctgtgca aacacgccat
    12421 gaatgcaggc ctcgatgact ttaaccgtac tgcccgttca tcaacgcccc ccatgagcga
    12481 atcctcacgg acagagaacg gcaggtatac cgtcaccgtt tcaaatggca gtgccacctg
    12541 cgggagtgtg agtctcaccg tgaaagggaa tggcagcaac aaccagtccg cactggacta
    12601 ttttttcaat cccttcattg aaacggagta tcagggtgtc ctgacggcac aacggtctgc
    12661 catggatacc atgctccgtg acctggacag cgcagcacag gaatttgtgt cagcgtaccg
    12721 gacccgacgc gacacgggta acggcaacct gccggatatt gaaagccgta ttcagaaggc
    12781 tgccaacagg tatgaagaaa cggtgcagaa ggcgttgcca acagataacg cagaaactga
    12841 gcgcagggag cagctgaaat cctacctgac cacctacggc tgggttgccc tgggggcctg
    12901 gtaccagacc tttgccactg ccaaccagcg actggcagaa ctggccgacc gtgcccccac
    12961 tgtgagtgga gtttcatcgc agggcgaagc aggggatact gaattcctga aggaagtaaa
    13021 aggagcttac caggcacagt tgcaaaactc accatataca cctgcactgg gaacagccac
    13081 ctcagccagt gaataccagg catctaatac tcaagatcct caatcagcaa tcttaaaact
    13141 gataggattt aacctaacaa gaatgactaa ctggacagct acttatctga gcggtacagg
    13201 tactacaact aatcaactaa acccgttgat aaaaatgaaa aatattggtg attacaccat
    13261 ggtcacaacc gaaaccatct gggcgggtta taccaccgtc agggtgcttt ccactgccgg
    13321 cagcaactcc atttttggtc gactggtcaa tgctgttagc ggcttatctg atgccgcaaa
    13381 ggcttttctg gaagcagctg caccaccgct ctacttcctg ctgtttttgc tgttctgtgc
    13441 cggtttttcg ttatccattt acctgccgtt tattccgttt atcttctgga tgaccggcat
    13501 cggtaactgg atagtcagtg tactgattgg atgcgccgcc ggccccctgt gggccgcaac
    13561 ccacctagga acaagtcagg acagaggaag tcgggccgct tacggttaca tctacctgat
    13621 agacagcatg atcaggccac tgctgatggt gttcgggttc ttttttgcat cggtcgccat
    13681 cgtggctggc ggcaccatac tgaatgctct gtacgctgca gcactaatta atgtccagat
    13741 aaattctgtg acgggggtgt tcagcatggc gggatttttg atgatttatg cccgcgcctg
    13801 taccacactg gttgcgggcg tttttgcact tcaggcttat ctgccagacc atgtgattaa
    13861 ttttctgggt ggacgtgacg gcgttaatat gctggggaat atggtttctt ccgtcaaaga
    13921 tattattgcc ggcggaaaca ccaatatacg cagaacacct ggcgttcgtg aagaaaaact
    13981 gaaaaatatt aatggagatg ataaggacgg aataaaacaa tagagtacaa aacagtattg
    14041 ccatattcac ctgaagagat taaaatacat tcaggtgaat atgggggttc attatgaata
    14101 caacaaaaaa cagatatcct tcctggtggg gatattatta tcttattcgc aacacttact
    14161 ttatatttgg cattcctttt ttattgttat gttctctagg ctttaccggc atgatgctta
    14221 cagaaccata tataggaaca gatattcaag catatctata tacttgtggc agttggtttc
    14281 tattaataat tccttcacta tggctatatc tgagaactaa ggcaaagaaa aagaaaatcc
    14341 tacaggttgt taagaagata aaagaaagca gtccctttgc gccgactagc gattacgagc
    14401 aactgtcatt ctcgaagagt tgttatttcg ggatcgacat taaaaacggc accatgctgt
    14461 atgtgcgcat ttacccaaac aatgtcatgg atgtgattgg tctggatatt cacaacttca
    14521 cccgaacagt tgctgaagac ggcaagctgg aaatccacac cacctatgtg agtctgccga
    14581 tgatccctct tgaaatctcc ggcatctcat caaggacact ggccaacacc atgcacacca
    14641 tggctgcacg tggatatgag tacaaggaac gtttcccgca gatgatccgg tatcgggtta
    14701 aagagtggga aaaagttgcc ggtgtgcctg ttgcagaagt attttaaggt agcgaccaac
    14761 tgttagacgg taatccgtca gccggttttc tctgaaccgg ctttttcttt tcaggcagaa
    14821 tgcctgtctg ccgtctatct caggacgccg catggagtct ctattttttc cccacaaatt
    14881 cttagcactg tcttgcccac tgtagacaaa atcaatacaa ttctgcatat cagttatcta
    14941 taaaagataa caatatcatt ataaataatt atcatatcca cacactcaag gcagtaccta
    15001 gaaaacataa agataaaatc atgtctgaat cccgcagcaa ttttccagca cgacatggta
    15061 ctccatggaa tactgaagag ttacagcgtc ttctcgtatc catagcggag ggcgtctcgc
    15121 taaaaaaact tactcaaaca catcaacgat cgtccggggg aatacgtgga gcaatcaaac
    15181 gcctgcttcc ttctgagttt cttgagttca acgatataaa cactgtgaat gatctcagca
    15241 attacttcaa caagattcct cgccaagaac acaatagtat catcaataat ttaatatcat
    15301 tgagcactgc aaaaacaatt gctcatgata aagttgaaat acaaaagaat agttttaaac
    15361 aaaatacccc actccccctc tgccaaccca atacagattt taaagattat atagccgatg
    15421 aacatgatgt cattatgcta attcattccg ctatcgaaag tttatctaat aaacgacatc
    15481 aatatattct tctaatgcga ctcggcacag atgattcacc tcattctctg gcagatattg
    15541 ccgtcaaatt aggaatttcc cgtgaaaggg taagacaact tcaggaacga gcccttggct
    15601 tactagctgc taaatctcgc tatgaaggga caccaggagc ttgccttaaa gcccttatta
    15661 actcaatagg taattcacca tatgatatag cagtatggat ttatcgtatt gttcatcggg
    15721 atttcgttac atcctctgag ttagccgcca aattcattat ttttaccgca ggcattccta
    15781 aagcaagacg agatgaaata aaaaattcgc tctcattaat atcacaatta caaaagcaga
    15841 ggttacgtga acgaagagca gaattagcac acgctcgcgc tcaggaaaga gcccgaatca
    15901 agaaagaaag agttgagttc atattcagta aatggctaga caatacacat tggccaaaat
    15961 atatcgcacc tcctcccccc gtagaacagc tatgttcaca gagagcggtt aatgatactg
    16021 acatatctgg attcttttac tctcaaaaat taggtcgtac agtccagtac gaatcagggc
    16081 ttgagtttaa aataatgaat ctattagaat gttgcgatca ggttcttttc tatcaagaac
    16141 aaccattttc tattccatat tgttttaaaa acaaatcaaa aaaatactat ccagatttac
    16201 tcattgcgac agttgatggt cggtgcctac ttatggaagt gaagccaaca gaccgaatga
    16261 ccttgagtct cacgagaata aaaagtaatg caggtcgcaa atgggcacat actcgaggat
    16321 ggggatggtt ggtcattagt gataggttct cgttacgtca gctggaagaa catgccatct
    16381 caacaaacac atggaaactt atagaggctg aactgaaaac atcaaggatt ttagcatggc
    16441 gtgaaatgat ggagttacgt acacgatacg agattcatcg tttagatctt attgcatata
    16501 tcattcagtc tggtgctgaa gtggataact tttatcgaat taccccgaaa atatctaata
    16561 atacatctaa ccagagacaa ccaaatgaga gcgactgtca taattgatga cgcacaggac
    16621 ggaaaaaata atagctgccg gacagcagca accagcagtc gtccggtaaa aaagacaaac
    16681 aaggcgcatt gaggtcagcc ttcgcaacaa tccggcggca gatttcctgc cgccgtgttg
    16741 cccgttaacg tttaacttcg taggctaacg ttgccacaag cactgtgttt ccctgctcga
    16801 cacggaatcc gcaaagcgaa tccctcagca tccagacaaa acacagtatc gtcacacaga
    16861 cgacaattaa cattgttaaa aacgttcgtt gtggcatcgc agcccctcca ttgcctttcg
    16921 gccgtcatcg ggctactata atgttgtcta ggcattacag tgggccctgg gttgattgat
    16981 aaaaaaatcg cctggggctt tctgctgcat gccgtccaca aaacgctttc agcatacagc
    17041 ctcaagcgcc cgccgctatt ctaatcgatt ttcagtctgc cacaatcgtt ttccacctaa
    17101 tgacatgtta tgcacaacag tatttttacg ggaaactaca cggcaaggat tcccggaaca
    17161 cgttcattat tttatggtta cagacatcat gttaaatccg gaagtaataa tgacctggaa
    17221 ggaaatacta tgagtaacga atatcaaagc accagtataa acccggatgt ttttgaacaa
    17281 ttttttaccc tcctgaatac acactgtaaa tcatcagaag ctgcacttgc catgctgagt
    17341 acactctgct acgaactgat gaggaggagt gggatccatt cactggacag cctgatgctc
    17401 aacggagaag gtatattgat acaattacag ggggctgata ttcgcgaaaa aacaggtcag
    17461 catattcact gatccaggga tgtgctgaaa acagtgtcag cctgaaataa acgttcaatg
    17521 cctcatacac atgaggcatt gagcacggtc ataaataatc tttattattt ttctgattca
    17581 gcatttccgg tcagtatcgg ctcttctggc aaagacatta ccggaacgtc acgttcacca
    17641 ttgaatacag aaacactttc agcgaaatca ttctgtaaca aagaaaaact taacggcaga
    17701 tttttggttt gtatccctgt gatccattga aaaatctcgg gaaaacgacg tgcactgata
    17761 tgcacatgat tgaggtggtg ctcagtaata tcactggcca tcatcatgag aggtacatcg
    17821 tatcccgaac gcaaacctgg cgcatgatgc acaggggtgc tgtctgaact gacggattgc
    17881 ccatgatcag aaaaatacag catggcaaaa tcatcaccat gctctgtcgt catgttataa
    17941 attgcctgaa gaaaccgatc cagcttaagc aatgttgata aataacagga tactttattt
    18001 ccgaataatt ccctgaatgg ctgtgcatca ttaaaaagtc ggtcacaggg attagtgtga
    18061 gaccccagca tatgaataaa aatcactttt ggagaagacg agttaacagc ttccctgaca
    18121 tactgaagca tatcctcatc atcaatgcga agggaagaag agctcccttc cttaagaaaa
    18181 tgttttttat ccgcattggc ggcgatgaca gaaatcagcg tatcatgttg ccctaaagag
    18241 ccctgatttg atatccacca cgtctcatac ccggcttttc tggcaagcgt aacaatatta
    18301 ttacccggat tccctgtttc agtgtaatca agcgtcagtg ttctgctgag tgaacctatc
    18361 gtattcggcg ctgctgagaa atacccatta ataaatatac ccggagcaga attcagccat
    18421 ggtgttgtcg gcaaaggata gccataaaca gaaagataat cccgtctgac actttcacca
    18481 attacgataa ttatggtttt atatttcttc tccacactca caatatccca tgagtctttc
    18541 tgattgtttt tcagcgattc atatcgcatc atttcttctc tggtttcagc tatccccgtc
    18601 attacattcg taaccatacg ttttggccat gcatttactg acaatacgat aaagaacaac
    18661 agaaagataa atccccggtg gcttttatca cgcttacgcc agaaccagaa tgtaaaagca
    18721 gcaatgacca gcgaaacaat aaaataaaga acaggtatgc ctgtgataaa ctcacctgcc
    18781 tcatttatat ttgtctgcat tacagacgca ataaaactgt tgtttatttt cccgtaaatc
    18841 atcccaaccg gagcatagca tgcacttaac aaaagaaaga tacagacaca cgttgctgaa
    18901 ataacattct gccgccccaa caacaacatc aggaacaata taagcacatt ccccagggtg
    18961 tttttcactg tatatccgca gcaccatgta acaagcacag aaaacaacag ataaatcagt
    19021 gactctttgc accacaacat aaaataaaac tgttttgctc tctcaaaagc atattttgat
    19081 ttttctttaa tacaagacac gccagtcatt ttcctgtaca aacacagaca atcatttcaa
    19141 cgcatggata tttatatgaa acagctcaca aaaacatcct gttctgtcca gcaagattga
    19201 catattatca ggttctgtgt ttacagcaac tgctccccga accagattat tcgcataacc
    19261 cgatgaaggc ctcataacca taaaacactc agaatacatt tagttaacaa aaatatgtct
    19321 tgccctcccg gaaaggcaaa catacgaaag agaaagcatg gctataataa tatcaatcaa
    19381 aatatactca atctttccgg ttgtcataca ctcagcaccg acaacataaa gacaataaac
    19441 aaaagtgata gcatagtgcg atatataaag gagatgaata gccagaagta attttcctac
    19501 cactcttttt cgtttaaaga aagaataaat gaatagcaaa cataaccctg acagaaaaag
    19561 aaaggtagca atggagatta taaaatactc cgtcacctgt gaattctcac ccagagatga
    19621 atacagcgaa taatatgtag ttgtgagatt aagtcttgtt atcaatatat aaacaactga
    19681 taatagtata atataaagaa tcagaccaaa tctgtttttc tgcgctccgg ttaatacttc
    19741 tgatttatct cgcataaaat atcctgtctg taatctgtcc cggtaaacaa tggtaaattt
    19801 cagaatcagt gcgaatacgg taacaaagat tcctcctgtg ctttaaagtg tgtaggcaga
    19861 aaccagtctg gtggttccgg tcagggagtc tgatacctca acttcccggg tgactatcac
    19921 catacctgac tgacgcagtc tctgcacatc agcggcaatt cgctgagttc ccagccagaa
    19981 caggccatcc agtgccgtta ccagcaaccc gctttccagc gccagtttca gccgtgccgt
    20041 cgggacttta acctgcccgg cgatatcagt aaaacaacgc gtcgtcatgc catagcagtc
    20101 catccgccgt attcgcgtcg caagactgta gctgaaacaa tccggcacgc cggataagtc
    20161 attctgcaga ctgtatacac atccccgctg ccgcccgttg aacagggctt ttttctgact
    20221 gagcaaccat tcccgacgca gagtgtcaat catctgcggt gtccgggtta acagcagacg
    20281 aaacggcagc tcaaaactgg tcacataatc aacattcagt aatgctgtcc gcagtcgctc
    20341 cctggcacct gccccggtct cccggcattc acgcatgaca tcagcccact gacgcatcag
    20401 tcgtggagac tcattcctgg ccctgaactg atatttctca atatgataat caatacgttc
    20461 ctgcatatcg ccaccagata tctgttgttt acccagacac agtgattagc ctacaggaat
    20521 aaatatcctc cgtgaagcca ggtgcctcag aagataaaaa catgtatttt ttcgacgcgt
    20581 aatattaacg tcatccccat aaaaataaat caggaaaata taaaatgata aaatatatat
    20641 tcgcactcac tcttctgctt tccggttctg tctgtgccgt tcaaattcag ggactcgtta
    20701 ttcgtgtgct ggacggtgat actctggaga tattacaggc ccggcacccc gtgaggattc
    20761 gtcttgtgaa tattgatgcg ccggaaaaga aacagccttt tggacgctgg tcaatgaatc
    20821 aacttaaaag ccttgtggca aatacgcccg taaccgtgac atacacacag acagaccgct
    20881 acgggcgtat tctgggacgg gtatataccg aagacggcat ggaagttaac cgctggctgg
    20941 tacagcatgg tgcagcctgg gtatatcctg atttcaacac agattacacc ctgccggcat
    21001 accagagaga ggccagaaca atgaagcggg gtctgtgggc agataaaaaa cctgttcctc
    21061 catggctgtg gcgaaaagaa aataaaaatg accacacaga attacaaata aacaacatct
    21121 taccgtaata tgtatttttt ctgctgcatt atcttccctg tttacagtca gcaaaaaaac
    21181 gtccgaagca aaacaaacac gaataataaa cctgcccgat gcatccatta caaaaatgaa
    21241 tccttcctct tatggaaaat acagtaacca
//
LOCUS       DAZUFB010000084        21200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZUFB010000084 DAZUFB010000000
VERSION     DAZUFB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.084"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            225..725
                     /gene="tssB"
                     /locus_tag="ACHY3M_003462"
     CDS             225..725
                     /gene="tssB"
                     /locus_tag="ACHY3M_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFS4318307.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            760..984
                     /locus_tag="ACHY3M_003463"
     CDS             760..984
                     /locus_tag="ACHY3M_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318308.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1035..2509
                     /gene="tssC"
                     /locus_tag="ACHY3M_003464"
                     /pseudo
     CDS             1035..2509
                     /gene="tssC"
                     /locus_tag="ACHY3M_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2516..2929
                     /gene="tssE"
                     /locus_tag="ACHY3M_003465"
     CDS             2516..2929
                     /gene="tssE"
                     /locus_tag="ACHY3M_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS4318309.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2933..4783
                     /gene="tssF"
                     /locus_tag="ACHY3M_003466"
     CDS             2933..4783
                     /gene="tssF"
                     /locus_tag="ACHY3M_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS4318310.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVNEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4747..5828
                     /gene="tssG"
                     /locus_tag="ACHY3M_003467"
                     /pseudo
     CDS             4747..5828
                     /gene="tssG"
                     /locus_tag="ACHY3M_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            5853..7133
                     /gene="tagH"
                     /locus_tag="ACHY3M_003468"
     CDS             5853..7133
                     /gene="tagH"
                     /locus_tag="ACHY3M_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFS4318311.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAVRCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7130..7654
                     /gene="tssJ"
                     /locus_tag="ACHY3M_003469"
     CDS             7130..7654
                     /gene="tssJ"
                     /locus_tag="ACHY3M_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS4318312.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7657..8988
                     /gene="tssK"
                     /locus_tag="ACHY3M_003470"
     CDS             7657..8988
                     /gene="tssK"
                     /locus_tag="ACHY3M_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS4318313.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LTLPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8993..9754
                     /gene="icmH"
                     /locus_tag="ACHY3M_003471"
     CDS             8993..9754
                     /gene="icmH"
                     /locus_tag="ACHY3M_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFS4318314.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMTEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9763..12570
                     /gene="tssH"
                     /locus_tag="ACHY3M_003472"
                     /gene_synonym="clpV"
     CDS             9763..12570
                     /gene="tssH"
                     /locus_tag="ACHY3M_003472"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS4318315.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESEQDNTGAEPADEAGSEQPEETAETV
                     SPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDL
                     TKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAE
                     LLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDE
                     VEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQ
                     EVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIE
                     PEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGA
                     FTADVEDAQNDESVTKDETVL"
     gene            12567..13310
                     /gene="vasI"
                     /locus_tag="ACHY3M_003473"
     CDS             12567..13310
                     /gene="vasI"
                     /locus_tag="ACHY3M_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFS4318316.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13315..14727
                     /gene="tssA"
                     /locus_tag="ACHY3M_003474"
     CDS             13315..14727
                     /gene="tssA"
                     /locus_tag="ACHY3M_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS4318317.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWHQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADREPSLLEQLEQKFTAEQ"
     gene            14836..18269
                     /gene="tssM"
                     /locus_tag="ACHY3M_003475"
                     /pseudo
     CDS             14836..18269
                     /gene="tssM"
                     /locus_tag="ACHY3M_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18280..19729
                     /locus_tag="ACHY3M_003476"
                     /pseudo
     CDS             18280..19729
                     /locus_tag="ACHY3M_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19656..20138
                     /locus_tag="ACHY3M_003477"
     CDS             19656..20138
                     /locus_tag="ACHY3M_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS4318318.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20182..21096
                     /locus_tag="ACHY3M_003478"
     CDS             20182..21096
                     /locus_tag="ACHY3M_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318319.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21106..>21200
                     /locus_tag="ACHY3M_003479"
     CDS             21106..>21200
                     /locus_tag="ACHY3M_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318320.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTG"
BASE COUNT         5075 a         5701 c         5793 g         4631 t
ORIGIN      
        1 gcgaattgca caggttcaca caatctattt aacgcttgca gaatacccct ctttacgatc
       61 gataaaaagt ccttttctta tttattttga agcgtaaaat gcggggtgat gaaggagtgg
      121 tgattttatt ttcaccgcca ttagggcggc attgccgata tttttttgcg gtacaaatat
      181 gccgtgttca tggcctgtac gagataacat gacggatcac ttttatgagc aaaaaatttg
      241 aaggcagcgt cgcgcctcgc gagcgtatta atatatcgta tgtcccaaaa acagatggcc
      301 agacggctga agtggaactt ccgcttaaca tgctcgttgt cggcgatacc ggaaatacgc
      361 aggaaacatc cccactggat gaacgtcagg cagtttccgt taacaagcac aatttcggtg
      421 cagtgatggc tgaagcagcc attggcctca attttactgt ccccgccacg ctgaagggaa
      481 gcacaacaga cgatgaactg aatgtggcgc tgaatatcaa atcactggat gatttttcac
      541 cagatagcgt ggcccggcag gttccggaag tgaataaatt gctggaactg cgtgaagcac
      601 tgactgcact gaaggggccg atgggtaacc ttccggcatt ccgtacccag ctccaggcat
      661 tgctggaaaa tgaagagtcc cgcgagcagt tgctcaaaga aatcggtctg gtgagcaata
      721 aataaccacc actatttcat taaacagggt tatccaggca tgacggcaca caaaaatatc
      781 tccgggacat accacctctc tgttgcagac attctgcagg tggtttacca ggtatgtttc
      841 agcccgtccg ttgaaatcaa ccaggatggt gtggctgcgc tgattacaac actggatcgc
      901 cgtatcagtg atctattgga tgagattatc catttttgtg agtttcagca gagcgcgtcg
      961 cactggcagc gcgtacttca ctgatggcga aaagccggtt tcagaaacac agctaaaaca
     1021 gaaggatttt cagtatgtct ttacaggaag aggaactcgt ttccagtcat gccgggcagc
     1081 ctgagcaggc atcgtcgctg cttgatcaaa tcatggcaca gacccgtatt cagcccggtt
     1141 cggaaggtta tgacgttgcc cgccagggtg tgacagcctt tattgccagc atcctgcaat
     1201 ccactgcatc tgccgaaccg gtcaataaac tggccgtgga cagcatgatt gcagatattg
     1261 atgaacgcat cagccgccag atggatgtca tcatccatgc gcctgccttt cagcaggtcg
     1321 aatcgttctg gcgctcactg aaaaccatgg tggatcgcgt tgatttccgt gaaaacatca
     1381 aggtcaacgt gcttcatgtc accaagcagg aacttctgga agactttgag tttgcgccag
     1441 aaattattca gtccggattt tataagcacg tttattcgtc cggtttcgga cagtttggcg
     1501 gtgaacccat cgctgcgtgc tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa
     1561 actgctgcaa tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt
     1621 ttccccggag tttatggggc tgaactcgtg gaccgaactg cctaatatca aagatcttta
     1681 tgccatcttt gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact cggaagattc
     1741 gcgctattta gggcttacgg caccccgctt cctgcttcgc cagccctatt caccaacaga
     1801 taaccctgtt aagaacttta actattacga agatgtcagc cagaaccacg aagattatct
     1861 gtggggtaat acggcctgga tgctggcatg caatatcgca gacagttttg ccaaataccg
     1921 ctggtgtcca aacattatcg gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca
     1981 tctgttcgaa acgatggggc agattcaggc caaaatccca accgaggtgc tggtcaccga
     2041 tcgccgcgaa tttgaactgg ctgaagaggg ttttatcacc cttaccatgc gtaaagactc
     2101 tgataatgca gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa
     2161 agatgcagaa accaactata agctgggtac gcagcttcct tatctcttca tcatcaatcg
     2221 gttagcgcac tacatcaagg tgttgcagcg tgaacaactg gggtcatgga aagaaaggag
     2281 cgacttagaa agagagctta atacctggat ccgtcagtat gttgccgacc aggaaaaccc
     2341 gcctgcagac gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt
     2401 agaaggcgaa cccggctggt accaggttgc gctaagcgtg aggcctcatt ttaaatttat
     2461 gggggcaaat tttgagcttt ccctggttgg ccggttagac agggagtaac acaccatgat
     2521 accggatcgg gaaagaacca caggttctct ttttgagcgt atggaagcct cttcggccag
     2581 aaaccggcag ggaggcagta ttcattcgct caggcaatcc attcgccaga acctacgaaa
     2641 cattcttaat acccggtccg gtagttgccg gggtgcgcct gagctgggca ttgatgaacc
     2701 ggaaggggct gaaaacttcc gcgaatccat gagtcgggcc atcgagcaat gtatagaacg
     2761 ctatgaaccc cggatctcac acgctgaagt gcaggcagtg gtttcatccg cctccagccc
     2821 gctggatatg acgtttcata ttacagcctg ggtgacattt aacgaaacgc atgaggtgct
     2881 tgaatttgat atggcaccga atggcagcca gcattaccgg gtggactgac aaatggaatt
     2941 tgaagaacgc tatttccggg aagaactcga ttacctgcgc cagcttagca agctgctggc
     3001 aacggaaaaa ccccatctgg cccgcttcct ggccgaaaaa gatgcggatc cggatattga
     3061 acgcctgctg gaaggggtgg cttttcttac cggcaatctc cgccagaaaa ttgaggatga
     3121 attcccagaa ctgacgcacg ggcttattaa gatgctatgg cctaattacc tgcgtccggt
     3181 tccggcaatg acccttattg aatatacgcc ggatatggat aagtcttctg taccggtgtt
     3241 aatcccccgt aatgagcagt ttacaaccaa cgccggggaa atcagagtta atgaagtgct
     3301 gccctctgat gctaaaaagg aggagccgcc tccctgtacc ttcactctct gccgggatat
     3361 ctggctgctg cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt
     3421 tatcaacatc accttttcgg tcgcaccggg aacggacttc cgcacgctgg atctgaacaa
     3481 acttcgcttc tggctcggca atgacgacaa ctatacccgt gaccagcttt atttatggtt
     3541 ctgcgaatac ttgcagggtg ccgacctgac tgtgggtgaa cagcatattc gcctgcctga
     3601 gtttatgcta aaagctgtcg gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa
     3661 cgtccacagc ggctaccgga tccttcagga gtatttctgt taccccgatg cgtttctctt
     3721 ttttgatctt tgtggttgtc cggctttgcc tgacggattg caggcggaat tctttaccct
     3781 gcaactgcgt ttttcgcgcc ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg
     3841 cctgtattgc gcacctgcca ttaatttatt tatccaccat gcagaagcca tcacgctgga
     3901 caaccggcgg gcagactatc cgctggttcc cagccgccat tacccacaac attacgatgt
     3961 attttccgtt aacagtgtgg tgagccaggt ccaggatatg ttcaggaaaa aagatctggg
     4021 gcgtcctgtt tcgacgcagg ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca
     4081 gatggaatac agccggaagc gggaagtggt gtactggcat caccggacca aaacatccct
     4141 gttccatcgc ggctttgatc atacccttgc ctttatacat gctgatggca gttatccgtc
     4201 agacgaatct ctgctcagta atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga
     4261 gcttccgtca caaattcgtt ccggcgatat caccggcaca accggtaaaa atgcggctgt
     4321 tgcttcattt cgcaacatta cccgcccgac gcaaccactc tggccggtca ttgatggcag
     4381 cctgcactgg tccctactct ccgccatgaa cctgaattat ctgtcattac tggatacgga
     4441 cgcgctgaag caggtcatcg ccaactttga tcgccacgca atccatcatc cgcagacggc
     4501 acggctgtca caacaaaagc tggatgccat tgagcgtctg gagacccgcc ccgttgatcg
     4561 cctgtttacg ggtattcccg tccggggact ggcctccacg ctgtatctgc acccggagcc
     4621 gtttgtctgt gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct
     4681 gtacgccagc gttaactcat tccacatgct gaccgttgtg aacacagaaa gccaggagac
     4741 atggaaatgg acggaaagaa tcgggcagca tcctcttatc tgagtccggg aaatcccccg
     4801 gcagataaag agcagaatga tccgctggca caggttttcc ataatgcctg ctcttacaat
     4861 ttttttgcga tggcggagct gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa
     4921 ttatccctgc gtgacgatcc ggcacaggaa accctgcgtt tcagcgccga tgccagcctt
     4981 gccttcccgt gcagtgatat cagtgcactg aaacgggata cgtcaggggc attcaggatg
     5041 accaccacct ttatggggtt acaggggagc cagtcgcctc tccccggtta ttacctcgat
     5101 cacctggcct ggaaagcggt acatgaacaa agtccggtag gtgattttct ggatatgttc
     5161 agccaccgcc tgacccagtt tgtctggcat atctggcgta agtaccgcta tcacatcagt
     5221 tttcgtaacg gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt cgggctgggc
     5281 caccgccagc ttcgcgataa gctggcgatt aatcacagca aaatgctggc gtattccggg
     5341 atcctggcaa atccgggacg atccccggaa ataatctgtg gcctggtgtc ccactgcttt
     5401 gatttgtcag aagtgacgct gcaaaactgg caacgtcgca aggtggatat tgaactgatc
     5461 agcaaaatag tctggggagc tattctctta aaaatggcga aaaactggcg ggaagatcgg
     5521 tactgggcaa ctttgttctg ggtacacgag tgcccgatct cagcggaaaa ttccagctca
     5581 gtattaccag cctgacccga aaacagttcc tctcttttct gccgtccggc gaaaacttcc
     5641 tgccactgac gatgttcgtg tccttcatcc tgcgcgatca gcttgcctgg gacttacatc
     5701 tggggctggc gccggagcag gtgggcgcaa tgcgcctggg cgataacaaa agtgcgctgc
     5761 tgggctggac cagcttcctc ggcacaccgg aagaacgacc ctcagtcacc atcagggtac
     5821 ggtcgtaaat tatgataatc acggagcaga atatgccgga agagaaacta cagacgcttt
     5881 cattgcaggt cattaacggc agtgagctgg aaagcggacg ggcggtgcgc tgtctgttca
     5941 cacagcaggg aaatgtcggc catggccccg aatgccactg gtcggtacag gatcgtcagc
     6001 agagcattcc ggcccaggct tttaccgtta tcctgcacga tggcacattt tgtctgcgcc
     6061 cgcagacggc acaactgtgg ctgaatcagg caaaagtcac agcaacatca gacctaatac
     6121 agttgcgcca gggcgatgag atccagatcg gacggctgat ggtgagggtt catctgaacc
     6181 ggggagatat tccccattac gatgaggaaa tggccactcc cgaaaccatc gttaccaatc
     6241 gcgatatgct cacggatacc ctgctatcaa cggagggtac gccacactat ccgggaatga
     6301 ctcaccggca ccaacttgca gacaccgtgg taaacggttt ttctgccgat ccactccagg
     6361 cacttcagtc cgaaagcctg attaccacgg gcgatccgct ttcaggcatt gcggctgtcc
     6421 ggccatcggc accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg
     6481 atctgccgcc catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa
     6541 tggcgcaacg ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca
     6601 tgagcaattc cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg
     6661 caataaaagg tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc
     6721 gcccgctgga agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa
     6781 tgtttgccga aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc
     6841 tgcgcaatgt ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg
     6901 tcctgctgga tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca
     6961 gccatgaact gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt
     7021 acgaagaact ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg
     7081 cccaagtcta tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa
     7141 ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa
     7201 aatcagtgag gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc
     7261 ggaaataacc gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc
     7321 agccgccgtg gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga
     7381 ctatgaccag atagccagta cgccgctacc ggatgtgctg ggtaaaaact atatcgacca
     7441 ccaggatttc aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga
     7501 gaaaacgcag tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa
     7561 acaaatcgag acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca
     7621 aagcagcatc gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg
     7681 atgtggcagg aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac
     7741 gactacctgg ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa
     7801 ctcactctca acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc
     7861 acactgcccg acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg
     7921 cacccgcaac attttccgga cgagagaagc cgcaatatct acctcactct gccggtcgcc
     7981 agtgatgtga gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat
     8041 gccgatgtcc gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa
     8101 ctgacgccgc gcattatgag cggtgcagaa gatatgagcg cctatattac gctcccgctt
     8161 tgccgtatca gcgatcgcca tgccgatggc tccctgacgt tggatgacga ttttatcccc
     8221 tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg
     8281 gccattggcg gacgggcaag cgatctggca aaccgtattg gctctccggc gcagagcggc
     8341 atcgcggatg tggcggaatt tatgatgttg cagttactta accgtaacca gacccggttt
     8401 acccatcgcg cccgccgatc ccagctccac ccggaagatt tctaccttga tcttgctggg
     8461 ttgctgggtg aactgatgac ctttaccgag ccgtcgcgcc tgccctgccc gcttgatgtg
     8521 tatgatcatc atgatctgac caaaacattt aaaacactgt taccggaagt caaacgggcg
     8581 ctgcataccg tactgtcgcc aagagcggtc aatctgccgc tgcatctgcg tgacggtatc
     8641 tggcaggccg atatccatga cacagaactg ctgcaatctg ccacctttgt gctggccgtg
     8701 gcggcaaacg tgccggtcga tcagatccag cgtcagttta tccagcagtc gaaaatttcc
     8761 tcgccggaaa aaatccgcaa tatggtcagt gtgcagatcc ccggcattcc attgcgcgca
     8821 ctgatggtgg ctccccgcca gcttccttac cattccgggt tcagctattt cgaactcgac
     8881 aagagcggac aggcctggac agaaatggct gccgccgggg ccgttgcact gcatgtatcc
     8941 ggcagtttcc cggatctgaa catgcaactg tgggcgataa gagggtaaga cgatggatga
     9001 ggggtcactg tctctgccac catttaccgg atacgacgaa aaaagccagc gcgactatca
     9061 tctcgcgctt cgtggcaaca gcctgaatcc gatgatcgac gcagccacgc cgctcctcgg
     9121 tatggtgatg cgcctttcaa ccatgaacag ccagacgatg acggagcatc tttttgctca
     9181 ggtggtgacg gatgttcagg ccgtggagca actgcttcag gagcagggct acgaaccggg
     9241 cgtgattatc tcgtttcgct acatcctctg cacgtttatt gatgaagcgg cgctcggtaa
     9301 cggctggtcg aacaagaacg agtggatcaa gcagtcattg ctggtccatt tccacaacga
     9361 agcctggggt ggagagaaag ttttcatcct gctggagcgc ctgatccgtg aacccaaacg
     9421 ttaccaggat ctgctggaat ttctctggct ctgtttttct ctgggatttc gcgggcgcta
     9481 caaagtggca gcacaggatc agggggaatt tgagcagatc taccgccgcc tgtaccacgt
     9541 cctgcataaa ctacgcggcg atgccccttt cccgctgtta caccaggaca aaaaaaccca
     9601 gggcgggcgt tatcagctca tcagtcgcct gaccattaaa catatttttt gtggtggcgt
     9661 tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg ctggacagcc agacgcagga
     9721 catcctgcac cagctaaaca aattacttcg ataaggacgt ttatgatcca gattgatctt
     9781 cccacgctgg taaaacggct taacctgttc tcccgccagg cgctggagat ggccgcctct
     9841 gaatgtatga gccagcaggc agcggaaatt accgtcagcc atgtgcttat tcagatgctc
     9901 gccatgccac gcagtgacct gcgggttatt acccgccagg gcgatattgg catggaagag
     9961 ttgcgccagg cgctgacggt ggagaactac acaactgccc gttctgcgga cagctacccg
    10021 gcattttccc cgatgctggt tgagtggctg aaagagggct ggctgctggc gtcggctgag
    10081 atgcagcaca gcgaattgcg tggcggcgtg ttgctgctgg ccctgctgca ttcgccgctg
    10141 cgttatatac cgcctgctgc cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa
    10201 caggactttg tgcaatggac acaggagtcg gcggaatcag tcgtgccgga tgcagacggt
    10261 aaaggcgcag gcacactgac ggacgctgct gacaccctgc ttgcccgcta cgccaaaaac
    10321 atgaccgcag acgcccgtaa cggcaggctt gacccggtac tgtgccgcga ccacgaaatc
    10381 gacctgatga tcgacattct ctgccgccgc cgcaagaaca acccggtggt ggtgggcgaa
    10441 gcaggcgtgg gtaaaagcgc cttaatcgaa gggctggcgc tgcgcatcgt ggcaggccat
    10501 gtgccggaca agctgaaaaa caccgatatc atgacccttg acttgggcgc attgcaggcc
    10561 ggagcgtcgg tgaagggtga attcgaaaaa cgtttcaaag ggctgatggc agaggtcatt
    10621 tcctccccgg tgccggtcat tctgtttatc gacgaagcac ataccctgat tggcgcgggc
    10681 aaccagcagg gcgggctgga tatctccaac ctgctcaaac cggcgctggc gcgcggcgag
    10741 ctgaaaacca tcgccgccac cacctggagc gagtacaaaa aatacttcga aaaagatgcc
    10801 gccctgtcgc gccgcttcca gttggtgaag gtcagcgaac ccaacgctgc cgaagccacc
    10861 attattctgc gcggtctgtc ggcggtctat gaacagtctc acggcgtgct gattgatgat
    10921 gacgccttgc aggccgctgc gacattaagc gagcgttatc tctccgggcg tcagttaccg
    10981 gacaaagcga ttgatgtgct ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg
    11041 ccgccgaagc aaatctcggc gctgaccact ctgagccacc agcaggaggc ggaaattcgc
    11101 cagcttgagc gcgagcttcg catcggactg cgtaccgaca catcacggat gaccgaggtg
    11161 ctggtgcagt atgatgaaac gctgacggcg ctggatgaac tggaagcggc ctggcaccag
    11221 cagcagacgc tggtccggga gattattgcg ctgcgccagc agttactggg cgtggcagag
    11281 gacgatgcgg cgccgttgcc ggacgcagat accgtggagg atacgcagcc agagtcagag
    11341 tcagagtcag agtcagagtc agagtcagag tcagagtcag agtcagagca ggataatacc
    11401 ggtgccgaac cggctgatga agctggcagc gaacagccgg aagagaccgc tgaaacagtt
    11461 tccccggtac agcggctggc ccagctcact gccgaactgg acgccctgca taacgaccgg
    11521 ttgctggtct ccccgcacgt cgataaaaaa cagattgcgg cggtgattgc cgaatggacc
    11581 ggcgtgccgc tcaaccgcct gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa
    11641 tggctgggtg acaccatcaa aggccaggac ctggcgattg ccagcctgca taagcatcta
    11701 ctgaccgcac gcgccgacct gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct
    11761 ggccccagcg gtgtgggtaa aaccgaaacc gtcctgcaac tggcagagct gctctacggc
    11821 ggtcgccagt acctgaccac catcaatatg tccgaattcc aggagaaaca caccgtctcg
    11881 cggctgattg gttcgcctcc gggctacgtt ggctacggtg aaggcggcgt actgaccgaa
    11941 gcgattcgcc agaaacccta ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg
    12001 gatgtgctca acctgttcta ccaggcgttc gataagggcg aaatggcaga cggtgaaggc
    12061 cgcctgattg actgcaaaaa tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta
    12121 atagtcgagc atgccgatga cccggaaacc atgcaggaag tactgtatcc ggtgctggcc
    12181 gacttcttca aacctgccct gctggcgcgt atggaagtgg tgccgtacct gccgctgtcg
    12241 aaagagacgc tcgccaccat cattgccggg aaactggccc gcctggataa cgtgctgcgc
    12301 agccgctttg gcgcggaggt gattatagaa ccggaagtga cggacgaaat catgagccgc
    12361 gtcacccgcg cggaaaacgg cgcgaggatg ctggaatcgg tcatcgacgg caatatgctg
    12421 ccgccgctct ccctgctgct gttgcagaaa atggcggcga atacggcggt tgcccggatt
    12481 cggttgtcgg cagtggacgg cgcatttacg gcagacgtgg aagatgctca gaacgacgag
    12541 tccgtcacaa aggatgaaac ggttttatga gcaggttgcg cgttttccgg cagacaggat
    12601 ggtttatcgc gggcctgatg acgggcttac ccgccactgc cgcgcccgca gaagcatcgt
    12661 caatggccgg tgtggcggtc gccgtcgcca ccacgacccc gccggatgcg acagccacgt
    12721 tacaggccat gcagtcctgt cggcgggagt ccgcggcgct ggagcggctt gactgctatg
    12781 accgcctgct ggcaccgctg tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt
    12841 ttgtgggcga ggcctggacc cgcgccacag agcaggaaaa acgccgcgag ggcaacacca
    12901 cagaactgct ggtgacgcag gtgccggggg agcgcccgac ggtggtgata accaccccgg
    12961 ctatcggtca tgttccgcca cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc
    13021 ggatgcaggt ggcgctgatg caccctctgg atgtccatga tatcgccgtc actctgaatg
    13081 ccgacaaccg ggcattgcgc agccactggt ttgtgcgcga gaacggcacc ctgctggagt
    13141 ccagccgggg gttatccggg attgatgaaa ttaagcaact gttcggcgca aaaacgctga
    13201 cggtggatac cggcgcagac aatgccgccg gaaagctgac ttttaacatt gacgggctgg
    13261 caagggcgat tgctcccctg cgcgatgcct gccactgggc gggagaataa aaatatggca
    13321 atggacttac gcgatccgaa tgtctggata tcgcacctgc tggaaaacct gccggaagag
    13381 aaactggcat cggcgctgaa agatgacaac ccgaactggg agtatatcga cggcgaaatc
    13441 gtcaagctgg gatcgctggc ccacagtcaa ctggatatcc cggaactcca gcgccgtggg
    13501 ctggtcattc tggcttcgga gagtaaggat tttcgtctgc tggcgcatct gctgagaacc
    13561 ctgcaacacg ccggtgatcc tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat
    13621 tactgggcgg tggctgcgcc gcagaatatg gcgcataaaa aacgctttgc cagccaggtc
    13681 attaaacgct ttgaaacagg tattgaagtt ttttcacaga acgccgccac agcgcagcgc
    13741 gatgcgctgt caggggaact ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg
    13801 gaactggcac aggctactga tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg
    13861 gcaccggctc ctgtccccac tccggcggcc tccggtagtt caccacagac caccgccacg
    13921 tctgaaagcg gcgtgacgca acccagtgct ccggctcccc aaatcgtcat cgacagtcac
    13981 gacgacaaag cctggcgcga cacgctgtta aaagtggcgg ccattttatg tgaacgccag
    14041 ccggattcac cgcagggcta ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt
    14101 acgccccagg cggaaagcga tggccgtact cctctggctg cggtctccgc cgatatggtg
    14161 gcagattacc atgctcagct cggtagtgcc gatatggcgc tgtggcatca ggtggagaaa
    14221 agcgtactgc tggctcctta ctggctggat ggtcactgcc tttctgcaca gacggcgctg
    14281 cgtctgggtt acaaacaggt ggcagacgcc atccgcgatg aggtcatccg cttccttgag
    14341 cgtctgcccc agcttaccgg gctgctgttt aatgaccaca caccgtttat cagtgagcag
    14401 acgaaacaat ggctggcagc ttcgcccgac gccaaagttg caccggttgc gcaaatcggt
    14461 gaggaatcga aggcagccag agcctgtttt gctgagcagg gtctggaggc ggcgctgcga
    14521 tatctggaca tgctacccga aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc
    14581 gcacagttga cggaagaggc ggggctggta cagcttgcgc agcagcagta ccggatgttg
    14641 ttcaggatgg gattgcagat gatggtggca gacagggagc cgtccctgct tgagcagcta
    14701 gaacaaaaat tcacggcaga acaataaaag agaacaggag tttttgtgtt cagattacca
    14761 acaccccgac tactcagcgg actcaaatca gccctgcgac cggcgatgcc ccggtttaaa
    14821 gtctctgctt tctggctgct gatactggcg tggatttttc tgctggtgtg gatctggtgg
    14881 aaaggcccga tgtggacgct gtatgaagaa cagtggctca aaccactggc gaaccgctgg
    14941 ctggcaacgg cggcgtgggg gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag
    15001 cgcctgcaac agcttgaaaa aatgcaaaag caacagcgcg aggaagccgt tgatccgctc
    15061 agcgtggaac tgaacgccca gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc
    15121 catctcgaca accgccgttt cctgtggcag ttgccgtggt atatggtcat cggcccggcg
    15181 ggcagtggca aaacgacact gctgcgcgag gggtttccgt ccgacattat ttatgccccg
    15241 gagggcgcac gcggcgcaga acaacgcctg tacctcacgc cccatgtcgg taaacaggcg
    15301 gtgatctttg atatcgacgg cacactctgc gctcccgctg atgcggatat cctgcatcgc
    15361 cgtctgtggg aacatgccct cggctggctg aaggaaaagc gcgcacgcag ccgctgaacg
    15421 ggattattct gacactcgat ttacccgatc tgctgaccgc agacaaacgc cgccgcgagc
    15481 atctgttaca gacgctgcgc agccgcttac aggatatacg ccagcatctt cactgccagt
    15541 taccggttta cgtggtactg acccggcttg atttattaca gggttttgcc gccctgttcc
    15601 agtcactcaa cagacaggat cgcgatgcga ttctgggcgt cacgttcacc cgccgcgccc
    15661 atgaaaatga tgactggcga acagagttga atgctttctg gcagacatgg gtggatcgaa
    15721 tgaatctggc gttgccggat ctgatggtcg ctcagactca cacccgcgcg tctttattca
    15781 gtttttcccg ccagatgcag ggaagccgtg aaccgctggt gtcactgctt gagggtctgc
    15841 ttgatggcga aaatatgaac gtgatgctgc gtggtgtcta tctcacctct tcgcttcagc
    15901 gtgggcagat ggatgatata ttcacccagt ctgccgcccg ccagtaccgg ctgggcaata
    15961 acccactggc gtcctggccc ctggtggaca ccgcgcctta tttcacccgc agcctgttcc
    16021 cgcaggcatt actcgcagag cctaatctgg caacagagag ccgcgcctgg ctgatacgtt
    16081 cccgtcgccg cctgacggtt ttctctgcca caggcggcgt ggcagcactg ctgctcatca
    16141 ccggctggca tcactattac aacggtaact atcagtccgg catcaccgtg cttaagcagg
    16201 ccaaagcctt tatggacgtg ccgcctccgc agggggaaga tgactttggc aacctgcaac
    16261 tgccgctcct gaacccggta cgcgatgcca cactggccta tggcgactgg ggcgaccgca
    16321 gccgtctggc cgatatggga ctgtaccagg gacgacgtat cgggccttat gtggaacaga
    16381 cctatctgca actgctggag caacgttacc tgccctcgct gtttaacggg ctggtcaaag
    16441 cgatgaacgc cgcgccgccg gagagtgaag aaaaactcgc ggtgctgcgc gtaatgcgaa
    16501 tgctggagga caaaagcgga cgtaacaatg aggtggtgaa gcagtatatg gcaaaacgct
    16561 ggagcgaaaa attccacggc cagcgcgata tccaggcaca actgatgtcc catcttgact
    16621 acgcgctggc tcatactgac tggcatgcag agcgtcaggc gggcgacggt gacgccatca
    16681 gccgctggac gccatatgac aagcccgtgg aatcagcaca gaaagaactg agcaaactgc
    16741 ctgtctacca gcgggtttac cagagcctga aaacgcgggc gctgggcgtt cttcctgccg
    16801 acctcaatct gcgtgaccag gtagggccaa cctttgacca ggtgtttaca tctgccgatg
    16861 acaacaaact ggttgttcca cagtttatca cccgctacgg cctgcaaagc tattttgtaa
    16921 aacagcgcga tgaactggtt gaactgacag cgatggattc ctgggtactt aacctcaccc
    16981 gcaatgtgaa atacagtgac gccgaccgtg cggaaatcca gcaccagttg accgagcagt
    17041 atatcagcga ctacaccgcc acctggcggg ctgggatgga caatctgaat atccgcaatt
    17101 ttgagtccat cggacaactg accggggcgc tggagcaggt tatcagcggc gaccagcctt
    17161 tgcagcgggc gctgaccgtg ctgcgtgaca acacacagcc aggcgtcttt tctgaaaaac
    17221 tctctgccaa agaacgggag gaagccctgg cagagccgga ttaccagtta ctcacccgcc
    17281 tcgggcatga attcgccccg gaaaacagta ccctggcagt acagaaagac aaagaaagca
    17341 cgatgcaggc cgtgtatcag caactcaccg agttgcaccg ctacctgctg gcaatccaga
    17401 acgcgcctgt accagggaaa tcggcgctga aagccgtgca gttacggctt gatcagaaca
    17461 gcagcgatcc gatattcgcc acccgccaga tggcaaaaac gctgcctgct ccgctcaacc
    17521 gctgggttgg caggctggct gaccaggcct ggcatgtggt gatggtggag gctgttcatt
    17581 atatggaagt ggactggcgc gacagcgtgg tgaaaccgtt taacgagcaa ctggcaaata
    17641 actatccgtt taatccgcgt tctgcacagg atgcctcact ggatgccttc gaacgcttct
    17701 ttaaaccgga tggcatactg gataccttct accagcagaa cctgaagctg tttatcgata
    17761 atgacctgag tctggaggat ggcgataaca acgtcattat tcgcgaagat attattgcgc
    17821 aactggaaac tgcgcagaaa atccgtgaca tcttcttcag caaacagaac ggtctgggaa
    17881 catcctttgc cgtggaaacg gtatcgcttt caggcaataa acgccgcagt gtactgaacc
    17941 ttgacggtca gttagtcgat tacagccagg gccgtaacta taccgcccat ctggtctggc
    18001 ctaacaacat gcgcgaaggc aacgaaagta agctgacgct catcggcacc agcggcaacg
    18061 cgccgcgcag tatcagcttc agcgggccgt gggcgcagtt ccgcctgttc ggggccggac
    18121 aactgaccgg agtacaggat ggcaacttta ccgtgcgctt tagcgtggac ggtggcgcga
    18181 tgacctaccg tgtgcatacc gacacggaag ataacccgtt cagcggtggg ttgttcagcc
    18241 agtttggtct gtcagacaca ctgtactgag gaggcaggga tgaatagtaa cgtactgaca
    18301 caaactatcg ttaccggcag tgacccgcgc gggctgccgg aattcagcgc catccgcgag
    18361 gaaataaaca aagccagcca cccgtcacag cctgagctga actggaaact ggtggagtcg
    18421 ctggcgctgg cgatttttaa agccaacggt gtggatttac acaccgccac ctactatacg
    18481 cttgcccgga cacggacaca gggactggcg ggattctgcg aaggtgcgga actgctggcg
    18541 gcaatggtaa gccacgactg ggataagttc tggccgcagg gcggcccggc gcgtactgaa
    18601 atgctggact ggtttaactc ccgaaccggc aatattctgc gtcagcaaat ctcctttgcg
    18661 gaatccgacc taccactgat ctaccgcaca gagcgggcat tgcagcttat ctgcgataag
    18721 ctccagcagg tggaactgaa gcgcgttccg cgcgtggaga atctgctcta ttttatgcag
    18781 aacacgcgta aacggcttgg accccagctg aagagtaaca ctgagaacgc cgcacagacc
    18841 acggtcagaa cgctgattta tgccccggaa acacaggcat cttccacacc agaagcggta
    18901 gtgcctcccc tgcccggcct gcctgagatg aaagtggaag ttcgcagtct gacagagaat
    18961 cccccacagg ccagtgtgat aaatcaaggc agtacggtaa gagggtttat cgcagggatc
    19021 gcctgttcag tggctgtcgc ctcagcattg tggtggtggc aggtctatcc ggtgcagcag
    19081 caactgttac aggttaacga caccgctcag ggcgcagcaa cggtgtggat ggcctcacct
    19141 gaactcgaaa actatgagcg caggctgcaa caacttcttg atacctcccc ggtacagccg
    19201 ctggaaaccg ggatgcagat gatgcgtgtt gccgacagtc gctggccgga aagcctgcaa
    19261 cagcaacagg cctcgacaca atggaatgag gcactcaaaa cccgcgcaca gagtagcccg
    19321 cagttgcgtg gctggttgca gacccgccag gacttacatg cttttgcaga tctagtgatg
    19381 cagcgcgaga aagagggact aaccctttcc tatatcaaaa atgtcatctg gcaggcggag
    19441 cggggactgg ggcaggaaac acccgttgag tctctgttga cgcagtacca tgatgcccgt
    19501 gcgcagaagc agaatacaga tgcgctggaa aaacaaatta atgagcgact cgaaggcgtg
    19561 ttaagccgct ggctgctgct gaagaataac gtcatgccag aggcggcaac cggtaccacg
    19621 gccgaaaaat aattatttaa ttaaaggaga ttatcatggc aaatataagt tatttatctt
    19681 tgagtggaga gacacaaggg ctaatttcag caggttgctc aacgcttgat tctgtcggaa
    19741 acaaagcgca gccagaacat aaagatcaga tcatggttta tgcgttaatg cattccatct
    19801 caagaagtca aaatgtcaat catcatgaat taattatcac caaaccggtt gataaatcct
    19861 caccattgct ggcaaaagca cttagtgaca atgaaaaaat ggcaatatgt gaatttatac
    19921 tctaccgaac cagtaaagcg ggtatttacc agccttatta taaaataaat ctgtcaaaag
    19981 cccgaatatc aagtattgat tttgtcacac cgcatgctgt cctggaaaaa gaactggaac
    20041 cacaggagag aattgccttt atttatgaag atatttcctg ggagcatacg ttagccggta
    20101 cgaatgcaat gagtaaatgg caggatcgtg tacagtaact tactgtaata acataataaa
    20161 ttactattgc ggagagataa aatggctggt ttaaggaata acaacaacac acagaatgca
    20221 cagtgggctg attatgtggg tgacattctt cggggggctc agccgataaa ccagctagta
    20281 cctcagcacc cttatcttaa cgacgtccca ctcattgatg agttaaggca tcagaacact
    20341 catcatgtta ttctcttgac acttgatgtt gcaaaaaaaa tactcagccc aataacatca
    20401 tttgattaca ttcattttat cactacacat ccttcaggta taaaagatac actcgcctgg
    20461 ttagttaacg cgggtaaatt aatgactgag tttgatgaca atgggaaaat tatatttaac
    20521 cttaatgcct taaagtatac aaaagcaagt tactttgaaa tacttggaga gaaatatata
    20581 aaaatcacaa catccagccc ctggctttta gaaaagctcg gtaaatatat tttctcttca
    20641 cgagcgcctc aagttctgga actcgctatt ggctggcgag gagctttatc tgaatcaata
    20701 aaaggagtta agttctgtat ctggttttca gtagcctgga gaaccataga atttattatg
    20761 agttcagagc gagatctggt taattttctt ggcgattttt caatggatgt ggccaaagca
    20821 gttatagccg gtggtgttgc aaccgctatt ggaagcctgg cttcttttgc ctgtgttagc
    20881 tttggctttc cagtaattct tgtcggagga gcaattttac tgacagggat agtgtgtaca
    20941 gttgttttaa atgaaatcga tgctcaatgc catttatcag aaaaattaaa atatgcaatt
    21001 agagatggac taaaacggca acaggaactt gataaatgga aaagggaaaa catgactcca
    21061 tttatgtatg ttcttaacac tccacccgtg atatgaggat taaccatgcg tttacatgtt
    21121 aaattaaaag agtttctttc gatgtttttt atggccatat tatttttccc tgcattcaat
    21181 gcttctttgt tttttaccgg
//
LOCUS       DAZUFB010000085        21067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZUFB010000085 DAZUFB010000000
VERSION     DAZUFB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.085"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHY3M_003480"
     CDS             <1..99
                     /locus_tag="ACHY3M_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4318321.1"
                     /translation="FFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            101..370
                     /locus_tag="ACHY3M_003481"
     CDS             101..370
                     /locus_tag="ACHY3M_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4318322.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            510..698
                     /locus_tag="ACHY3M_003482"
     CDS             510..698
                     /locus_tag="ACHY3M_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318323.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            993..2618
                     /locus_tag="ACHY3M_003483"
     CDS             993..2618
                     /locus_tag="ACHY3M_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318324.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGA
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2615..3883
                     /locus_tag="ACHY3M_003484"
     CDS             2615..3883
                     /locus_tag="ACHY3M_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4318325.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3898..4176
                     /locus_tag="ACHY3M_003485"
     CDS             3898..4176
                     /locus_tag="ACHY3M_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFS4318326.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            4182..4799
                     /locus_tag="ACHY3M_003486"
     CDS             4182..4799
                     /locus_tag="ACHY3M_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318327.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4890..5624
                     /locus_tag="ACHY3M_003487"
     CDS             4890..5624
                     /locus_tag="ACHY3M_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4318328.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGFGSGDWRTSGVTAGIGLKF"
     gene            5857..5997
                     /locus_tag="ACHY3M_003488"
     CDS             5857..5997
                     /locus_tag="ACHY3M_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4318329.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            6054..6455
                     /locus_tag="ACHY3M_003489"
     CDS             6054..6455
                     /locus_tag="ACHY3M_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318330.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            6549..7205
                     /locus_tag="ACHY3M_003490"
     CDS             6549..7205
                     /locus_tag="ACHY3M_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318331.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            7208..7654
                     /locus_tag="ACHY3M_003491"
     CDS             7208..7654
                     /locus_tag="ACHY3M_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318332.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            7664..7915
                     /locus_tag="ACHY3M_003492"
     CDS             7664..7915
                     /locus_tag="ACHY3M_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4318333.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            7926..9191
                     /locus_tag="ACHY3M_003493"
     CDS             7926..9191
                     /locus_tag="ACHY3M_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318334.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGDKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            9261..17612
                     /locus_tag="ACHY3M_003494"
     CDS             9261..17612
                     /locus_tag="ACHY3M_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFS4318335.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDSNRWKD
                     NSTSFSLGDVLPTMGKGFARSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKVTGKAVSDGAKSSDEWLTSK
                     MSPGALRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKTLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLLNLAMRGTANTVKSGMIRNATEQGAINAAQGGYSRYQENMALRDTA
                     GMDVSPWEGVADATIEGGTLGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQGEAA
                     PQPEPVDPVAQHRESMQGMNREQLLEQYADADMAHEGDTSAVHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRPELLEEYRKLSLKEGRTDTEEQQLQAIRDVLRPQQEARPEAQP
                     QPENADDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNSYFTDEKSALG
                     SDVFLNASATGVKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGR
                     QGEILEGEPVRRGLPSPDEQNATAPVREGLPAPESQRGVDMPQPESLPRIVRDSLPEL
                     AQQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQARGAKIEDFGEEIKGAAKHRY
                     AQLAEAMGKTLEDGEYATQPLSKLFPKPDYARLEKEGVDSDTLAMMALYRSEIPTRTS
                     RNMQKWISIVKSGREATAGMLEGKIPAAKLADMMDNKSGLRSMSDTWKLLRTLSASQI
                     DKASGYRVRSGVYSFVGGKRYDPPQMMYSLRDSKGRDLFFSESRDELLKKAKAYFDEQ
                     GSRERETPATSADDRITFDVYRHKASGDIFIGYGKNRQKLKGGFESARDAHDYVRTHR
                     DELVNQVKALREVSREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYVEG
                     PRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAIN
                     LTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDA
                     EYPVRQEVYDAFKGVMKAINSSDMLRRSELLDGARSKPYWSTDVEMAARAFERYVQDK
                     ARMAGVENDYLVNIRKADDHGQPDTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGV
                     AFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVISGIKDSDLKVRVVKSQ
                     KEAEALAGESFDGYGKVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVV
                     TPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKF
                     VAAWVRVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGADASS
                     QYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPL
                     VISRDTVRKATNGVKHVVPMDVIERLPELMHNPDAIYRSATERNAVVMLLDAVDKNGD
                     PVVSAVHMKATDKRLEINKVASVYGTENGKKLKSMEMAGLTLYRKEKLSRDNPQYSGL
                     QLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAV
                     QENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLVKLLADYKIAQADLD
                     EYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVD
                     DMLARRRELIREAGLEESGVVDAWQKAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRE
                     SKQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDKDYWQVFTDDR
                     PDTMRAIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRA
                     MKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSD
                     GKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWF
                     NMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHAR
                     DAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDG
                     PLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPDHVKERNL
                     VIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAF
                     SPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLG
                     RRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISKTTDAA
                     VKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLRGSERTAF
                     IDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVD
                     RFNREYNKKVGVE"
     gene            complement(17834..18766)
                     /gene="yfdC"
                     /locus_tag="ACHY3M_003495"
     CDS             complement(17834..18766)
                     /gene="yfdC"
                     /locus_tag="ACHY3M_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4318336.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            19060..19815
                     /gene="mlaA"
                     /locus_tag="ACHY3M_003496"
     CDS             19060..19815
                     /gene="mlaA"
                     /locus_tag="ACHY3M_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFS4318337.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(19997..21055)
                     /gene="yfdF"
                     /locus_tag="ACHY3M_003497"
     CDS             complement(19997..21055)
                     /gene="yfdF"
                     /locus_tag="ACHY3M_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFS4318338.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAK
                     YQNEINQLSAPGVNFDDI"
BASE COUNT         5392 a         4602 c         5992 g         5081 t
ORIGIN      
        1 ttttttcagc atttgcaggt caggtttggt gacgggccgt ggcaggatgt caaggggctg
       61 gatgaagtgg gcagtgatac aggcagaaca ggagaatgac atgaatattt tgagaaagct
      121 tatgcagagt ctgtgcggtt gcggaaagca tgatgactgt gaaaacgggc agtcgcttac
      181 agcacaactg cgactgggac cggcagacat tctggagtca gatgagaatg gcattattcc
      241 ggagcaggac agggtaatca cacaggtggt gatactggat gcagataaaa agcagataca
      301 gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg
      361 gatgaaatag ccgacagctt cacaaaaacc ggagcccggc tccggttttt gttgtcatgt
      421 ataggggggg gttattagag agtgaagtaa taaacatgtt aatacgatgg agtgaaggat
      481 gccgtgtaat tctggttcaa gagtttttta tgccggaaaa tcgcaggatt attctggata
      541 gtaaagaatc ctggttaata atctgtgata gtcagttggg ccatttaatg cgcagtatgt
      601 atcagggacg ccgttttatt cagctgaatc tggaaaaatt gaaaggggta catgatgtcg
      661 ccttgccagt gaaatgggaa ttcacacgaa gacagtgaat agctttctgt atacgggaat
      721 ggcgaaaaat ggactgtatg gtgtgagtgt gaaacatctt gcgtgtgcgg agtgatgctt
      781 ctcgttgcta cagcggcaat gataatgcag tgaaaaaagg ggagcaatat gctcccccaa
      841 accgaaagaa aattgcaata atcaatgaag ttatttagtc atcatcagaa tgtcatgcaa
      901 ggcattttgt ttcagtgatg ccgatcgcga ttttagcgaa ttccatcata aatcccctga
      961 tttttaagcc tgaagcagtc aaaggaattt ctatgcccta tatcgatatc accacgatgc
     1021 gtgggatgat gccgcgcgtt gtgacatcca tgctgcccga gcattccgct gtactggcgg
     1081 aggactgcca tttccggttt ggtgttatta caccagaacg tcagatatcc ggggttgaga
     1141 aaacattcac aattaagcca aaaacaattt ttcattaccg tgacgatttc tggtttgcat
     1201 ggccggatgt ggtggatgtg atccgcagtc cgatcgctca ggacccccac gggcgtattt
     1261 actacactga cgggcgtttt cctaaagtga cggatgcgac tattgccaca aaaggggacg
     1321 ggaatcaccc gacatcatcg tatcgtctgg ggatccccgc gccgacgaca gctcctgtct
     1381 gtactgttca gcagggcggt gatgtttctg acgataaccc gaatgatgat gaaacccggt
     1441 tttatacgga aacctttgtc tcagattatg gtgaagaagg tccgccaggt ccggcgtctc
     1501 tggaggtaac actccgtact ccgggaactg cggtacaact gacgctggct ccggtgccat
     1561 tgcagaatgc cagtattaaa cgtcgccgga tttatcgctc tgcatcaggt ggaggggagg
     1621 cggatttttt acttgtggct gaactggatg catccgtgct cagttacacg gacaaaatac
     1681 cggcgaaaaa ccttgggcct tccctggcga catgggatta cctgccgccg ccagagaata
     1741 tgacaggcct ttgcctgatg gctaacggta ttgccgccgg gtttgccggt aatgaagtga
     1801 tgttttcgga agcgtatctg ccgtatgcat ggccggaagt gaatcgtcac acgacggcag
     1861 aagatattgt agctatctgt ccgctgggaa cgtcactggt ggtggcgaca aagggggagc
     1921 cttatttgtt cagtggggta tcaccgtcca caatttctgg ttccaaaatc ccttcaatgc
     1981 aggcgtgtct gagcaggcgg agtatggttg cgatggaggg ttttgtgctg tatgcaggaa
     2041 caaatggcct ggtgtctgtt gatgcaaacg gtaatgtcgc gctggcgacg gaacagattg
     2101 tttcaccgga acagtggcag agtcagttta atccggcctc cattgtggct tatccctggc
     2161 gtggtgaata cattgcctgt tacacgaaac cggatggtaa gcaggatgtg tttgtattca
     2221 gtccggtgaa catggatatc cgttatctca gtacaccgtt tgactgcgca tgggttgatc
     2281 tcgcgaaaga tatgatgcgc gtggtgacag gagacaaaat gtcagtgctt gccggggggg
     2341 ctctgccctc cacgataagg tggcattcaa aaattttttc attacctgaa agaacctctt
     2401 tttcctgtat cagggtgaaa tctccggcgc ctgagcgggt ggggatcacc attatggctg
     2461 atgatgttcc tgtgattcat tttgcgccgg gtacgtttaa gggaagtgtg gtgagacttc
     2521 cggcagcaac cgggcaaaac tggcaggtga tggtatccgg attcgggcag gtggaacgaa
     2581 taaccctgag tacatcaatg tcggagatgc cggtatgacc agaaaaccgt ggcgtgcggg
     2641 gaaggattta tccactgttg tggagaatat ggaaattggc accgggcagc gtggtgacgg
     2701 acgtcacgca tttgtgaccc gtgaggaact ggttggtctt aaactcgccc ggcgtcgaac
     2761 atcgggtggt gcctcatatg cactgaatcc gggtattgag attgacagta ctttaatgac
     2821 tgttgatttt cccacaaaac cgctgaattt taaggcgacc ggtggatttg gctcggttct
     2881 tcttgaatgg gatatgccta attatcgcgg acattcactg actgaaatct ggcggggtac
     2941 ggaggatgac cttgctgatg cagtgctggt tgccacgacg ccggggcagg tttacggcga
     3001 tccggttgac cctggctggt cgggatttta ctggatacgt tttgttaacg cggcaggagt
     3061 gaaaggtcca tggaatgctg aaaaaggcac tcaggcacaa acacagatcg gcgtgaaggc
     3121 catcattgac cagatccgcg atgaggctgc aaagtcgccg gttgtgtccg agctgcgtaa
     3181 agaaataaaa aacgcgcagg ggcaggctgt aaaggatgct gcaattaaga caaccgaagt
     3241 tgtagggact ctcagggaag aaacgacaag aacgattggt ggtattgaaa cccgcattag
     3301 cacactggat tcgtcaacca gtgaatcgct taatgaggtc gacaagcgca tcactaaatt
     3361 ggataaagaa ggcggtgaag cttttcttgc aatgtggtca aaaaaagcgg gagttgatgg
     3421 tatcactgcg gggatcggga ttgtcgccgg aaaagacagt gaaggcaggc ctgtaagtca
     3481 ggttgcaatt tctgcgtcgc agttgtttgt ctttgacccg aataatccgg ataacacagc
     3541 ctatccgttt gcggtatcag gtggcaaggt agtgatcccg aaagcgatga tttatgacgc
     3601 ggtgattgaa acactggtgt cgcggaaggt tgtggcggat gaggtaaaag ccggggtaag
     3661 tatcacttcg ccagttatcc ggagtgccgt tattcagaac ggaaactttc aggttgattc
     3721 tcagggtaac ctgaatattg gaggcctttt cagtgttacg tcacaagggc aactgacaat
     3781 tcgttactct aatcagaatg taggactggt gatccgcaat gataaaattg aggtttatga
     3841 ccagaatgga cgactggctg ttcgcatagg cagattacgc tgatcaggag gtgagtattg
     3901 gaatacggtt ttgccattta taacagaaat aacgttaatg ttacgggcgt gctgactcca
     3961 gtatttttcc tggacagatt tacagcggag tctggctcaa agacgtacac taataaaccc
     4021 gacgggaaat cattgcaggc tgtatgttgt ttatttccct ggaataatgt atttgcggat
     4081 cggaaagtac cgaagataac cattaatgac aatacggtga cgtggtcgaa tcttgagcag
     4141 ggtatgggat cttatattta tacattctgg ggataagtgt tatgtacggt ttgagcatta
     4201 tgaagccgga tggcagcgta tggataagtc caggttttac gccgcagtgt ctgatcaaca
     4261 aaggcaccat accggcgact gaaaagtctt tttttaaaac atcaatcccg tcaggcaaaa
     4321 gttgtttttt ctttatcaga acagagaaga aggccgatgt catgtacacg catgaacaga
     4381 ttgatggata tcatgcacta aggcttcatg taattgtcag gggaacgaac cctggtgtta
     4441 cgacggttta tgctttcgcg aatatggtta ctccaccttc tgagtatggt atcgccatgt
     4501 ataacccgga cggtgagatg atttatcatg gcgaaatgat gctgcttgac gcgaagttaa
     4561 tacctgttga tatcaaattt gaaaaggacc ttggatatcc atgcgcaatc atgcctgcac
     4621 tggtcgggta ttataactgg aaaagaactc cttatgatcg accgatttat accacatcca
     4681 ctggtgctac aggaaataaa atatattcct gtgagcatta ttccggtggt gcaacatggg
     4741 atattcgaaa gccgtatata gataaggtcc tggttattaa tacatcagta tatgattagt
     4801 tgaagcgagt ctttaatatt catttaaaat gtctaaaaag atgtattatt aaaaagttta
     4861 gcgtgttatc tgaatacagg atatcttaaa tgaagagtat agcaacactg gttgtgtgtg
     4921 caatctccgg gattgcctgt gtaaatttat ctgcacatgc agcagaagga gagcatacaa
     4981 tttctctggg gtatgcgcac tttcagtttc cgggactgaa ggattttgta aaggatgcga
     5041 ctgctcataa cagggagact ttcagtcatt tcgtcaacag aaactacttt tcttcattgg
     5101 gcgaatatac agatggtcgg gtcagtggat atgaaggcaa ggataaaaat ccacagggca
     5161 ttaatatcag gtatcgctac gagataacgg atgattttgg cgttatcacc tcttttacat
     5221 ggacgcgttc tctcactaac tcacagacat ttattgatgt gcagtcagcc gatcatacca
     5281 ggaagattaa gaatccggca gcttctgcca gaacggatat cagggcgaat tactggagtc
     5341 tgttagcggg gccttcatgg cgggttaatc agtacatgag tttatatgcg atggcaggga
     5401 tgggcgttgc taaagttagc gctgacctga aaattaagga caatattaac agtagtggcg
     5461 gattttctga aagcaacagc acgaaaaaaa cctcccttgc gtgggctgca ggtgcacagt
     5521 ttaacctgaa tgagagtgtt acactggatg tggcttacga aggtttcggc tctggcgact
     5581 ggcgcacgag tggcgttact gctggcattg gcctgaaatt ctgacctgta tccggtaacc
     5641 gtttactacc cgctgtgatg gcgggttttt tattgcccgt acagggcaaa aaccgtaaat
     5701 tatgcgtggg tgcctttcgg ctgatggctg gagggtgaac ctgaaggcct gatgtggaaa
     5761 ggccccgagt caacttaacg ttaacccgag gccctaacac ttcgtacctt aagcaagtag
     5821 aaggttagcg cctctctgta aaaggagtca agcgctatgt cgcaaaaatc gcttatcacc
     5881 gtcacaattt gcatgacggt tatcttcacc atctggatgt tgcacggttc actgtgtgag
     5941 ttccggctga atttgtgggg agcggagttt gcggcgttct tacagtgtaa gcagtaggaa
     6001 aaccgcgacg gggacgagag tccccgtcaa ctggttgctg aggttcagcc gatatggcac
     6061 ccgtttcagg tgagagaatg aacgataaaa ttctctggta tatgcagcgt gttgtgagaa
     6121 attcccgcaa ccctgaattt atgaatgaag ttaaagacgc ctgccttaaa aagcaggcgt
     6181 tttgttttga ggcacctgat ggctttttgg tgctgcgttc tgtgctcagt gctgatggta
     6241 tcccttatgt tctggtgttg ctgggcgtgt gtacggggag taacagcgtt gagcgttacc
     6301 tgccggaggt gaagacatta acccatctgg ctggcggacg ctgggctgag ttccatacgg
     6361 caaggcgggg atttatccgg ctgggaaaac gactgggctt tgagcgaatg ccggatgatg
     6421 aggatggctt catggtgttc aggatagcgg tctgactgct acagttttca tcattgtgtt
     6481 taaaccaaca ttgtaattca cattctgacc ctgctccggc agggtttttt gttatccagg
     6541 gggccattat gggtggaagt aaaggcggtg gtgataccaa agtaaaacca acagcagcgc
     6601 aaatagcaca ggaagaagtg gcctggaaag ggtggcagga ttacaaaaat atcctccgcc
     6661 cggctgaaga taacttcatg gaaaaggtcg atgacctgaa cagtgagcag cagtacgaca
     6721 atattgctgg caccacaaat ctggggtatc agaaacagtt tggcgaagca cggaaggagc
     6781 ttgcgggtaa tcttgctcag tccggcgttg acccatccag tggtcgtttt aatgcggtaa
     6841 tgaatgcgaa ccagagtgac caggtaaccg ggcagattga cacaaccaca cgggggcagg
     6901 tatcgcaggc agataagtat gttgccgggc tacaggatgt tgctgctctc ggttctggtc
     6961 agaaggcgga tgcgttacag agttttaact ctctggcaga cagcagtctg gcaaaagcta
     7021 aatcggatgc acaggcggcg tttacgaaac agcaggggcg agcctctctt gttggcgctg
     7081 gtctgggtgc ggcaggtgca tatgcgatgc ataaggctgg cggtagcgga ggaagtggcg
     7141 gtgctaaaac acctggcacc ggcgctaatg ccattcagca tcaggctcag aactggagac
     7201 tgtaattatg gagtatggca aatacgaaac tctcgcaagg gctggttatt caggagcaga
     7261 ccgcccacag ggtgactggc agacgtcagc agcgctgaca cgccaacaat acgacgactg
     7321 gcgaaccaga tatttgcccc gcgtggcaag gctggctgac cttggggaga acaacagtct
     7381 gatgaatgca cagcttgcac gggtgggagg ccttgccact tccagtctcc gtacagcgca
     7441 gatggcgcag gataaccaga tggcgagata cggggtaaac cgcccggata atcccgacag
     7501 taatacgctg gggttacgta atgccctggc aattgctggc gcgaaaaatg gtatccgtga
     7561 agccgaacag gatcgccaga tgaatattct gacgggggct tctgcaccgg caagacagaa
     7621 actgagtgtt ggcggccaac tggtggcagc gtaagggggc aatatgggat acggtttact
     7681 ggatattgca aatcagtcgc ggcgtgaggc attacaggga ataagtgacg cagacagacg
     7741 acgtgaagaa attgaggctg cgaacaaaca gatggcggcg caacagaaag cgcagaacaa
     7801 gcagaatatc ggtacgggca ttggtacggg ggcggctatt ggcgcatccg ttggtggtcc
     7861 tgttggtgct gttgctggag cagtaattgg cggcattgct ggttctttgt tttaaggagt
     7921 ggtgaatgag cggatttgca caggggttac ttgccggatt cagcaccgtt gaccaggcaa
     7981 tgacccgtcg taaggagctt ggtctgcgtg aagcacagct tgcccggcaa cagaaaaata
     8041 acgagcgcga ttttgagttt gcgcagtctc agtttgaaca taataaaaac gttgatcagc
     8101 ggaactttga ttacagagcc aaagttgacg accgtaatta tgcactgaag gaaagggagt
     8161 ttaacgctaa ccagaattac cggaatgcgt cactgggtat ggagcagcag cgactccagt
     8221 tgcagaaata caaccagcga cggcttgagt ataacgatat gattgcccat agccagccac
     8281 taatggaagc gcttggaaaa gcaattgagg ctggcgatca ggaggctgca acgcgtctgt
     8341 tcgggcagct gccaaaggga catccattaa ttcttatgtc aaacgaaggc tatgcagcga
     8401 aagcgggtca ggccgtgatc aacctgcaaa aaatctttgg tgataagccg gacatggcga
     8461 tcgattcgct gaataccccg gaaaatctcg atgtgctttc cggcgtgttt gccccggaac
     8521 tacaacagcg tattggcatg cctgattcaa ccggggacaa aacgataaaa gaggccagga
     8581 ttggcagtat cgtaccagcg cagcaggaag ggtacgtact tattggcctt gatctcacat
     8641 acagcgatgg ctccaccgcg cataaacctg taacagaata cggcagtgcg caccctgatg
     8701 atcaaaccgt gctggcgata cccgttgata aggctatcgc tcaggtcagg gatcgcagca
     8761 aatttgcaga gatatcgaaa aattatggtt attttatgcc gaagcagcag ggactttctc
     8821 tgaaagagct tcagaagggg gccagcaacg tagcggcgga cgcgatcaag aatggcggta
     8881 atgctcaggc tgcggtggat gaatattatg ctgcgactgg ttcacaaccg catcaacaga
     8941 aaattcagca acaaaaactt cagcaacagg ttatcaactg ggcgggagat gatcctgata
     9001 agctgtcatt tgccagaaat gtagcggccc gtcagcctga aatgctggaa cctcagaatc
     9061 agaaattgct ggagaacggg tatgcgaatt ttctccgtat tcaaaaggcc aggggggaac
     9121 aggccagaga tgaaagtgct tcatctgcat ctcagtttat ccgtggactg aaacagaatt
     9181 acgcccagta attcacgata ttccattaat accatttcct gatgcccggc cattgtgccg
     9241 ggttttttta tggagtctgt atggcctatt cagaggaaca gcgtcctgag gcgcaactcg
     9301 gtaaccagaa tcgtaacagc ctgaacattc agcaacccgg cgaaactgac agctatgaag
     9361 catttttctc tgattcaaat cgctggaagg ataacagtac gtcgttcagc ctgggcgatg
     9421 tattgccaac aatgggtaaa ggtttcgccc ggtccgtccg gggaacaggg gaaatggccc
     9481 gtggactcgg tgatgcgatg attcagagcc cggtaaaaac aggggcgcgt attttaaatg
     9541 agttcagccg tatggggctg ccgggtgtcg caactgtgca ggatattttt gccggtggca
     9601 gcaggggggc tgatgaggtc atcgataccc tgcctgatgg caaaaacgcg gttacagata
     9661 ctgtcggtaa aggtctgaag gtcaccggta aggctgtcag tgacggcgct aaatcctcgg
     9721 atgaatggct gaccagtaag atgtctccgg gtgctctgcg tgcgctgaat acgccgatga
     9781 ccgaaggcta tgatgattct gcggtctggg tggcgaaggg cgtaaacctg attggtgccc
     9841 ttgtacctga tatggttgct ggcggtgtgg ctaaaaaggt gggtgatgtc acattgcgaa
     9901 aaacgctgac cgccgggctg gagaaaaaat acatcgcggc agggatgcag ccggaaagag
     9961 ccacggcact ggcagcagaa gctgtcgata aaaaaatgcc ggatttattc caggcgggcc
    10021 tgatcaccca ttccacagcc agtgcacagg ggcagagtgc aatggcggca gcagatgctg
    10081 ttcttaatgc ggattactct gagctggcgc agtcaccgaa atttcagcag acgtttttgt
    10141 ccattgacgc cgatccgcag cacgcacagc ttactgatcg ccagaaaatg gatctggcaa
    10201 aagagcgtgt tgccgatgag gtgcgcgcgc agctggcaac agatcctgaa ttgctggctg
    10261 tgaatgccat ggcggcaaaa ctgggcgatg ctcagttgct taatctggcg atgcggggca
    10321 cggcgaatac cgttaaaagc ggcatgatca gaaacgccac tgaacagggg gcgattaatg
    10381 cggcgcaggg cggctattca cgctatcagg aaaacatggc actgcgtgat accgccggaa
    10441 tggatgtgtc accgtgggag ggcgtggctg acgcaacgat cgaaggtggt actcttggtg
    10501 ctgcgatggg ggctccattc ggtgcggttg ccggatatcg tggcagacgt caggccgcag
    10561 aagaaaccgc catgcgtgat gctgaaaccg tgcagcaggg cgaagcggcc ccgcaacccg
    10621 aaccggttga tccggtggcg cagcaccgtg aatccatgca ggggatgaat cgcgagcagc
    10681 ttctggaaca gtatgctgat gcggatatgg cgcatgaggg agacacgtct gccgttcatc
    10741 gccgggaagc cgccagccag ctgttgaatg aactggatga acaggcgaag cgacaggcgg
    10801 tgatggatga gctgaaggcg aagccgcgcc ctgaactgct tgaggaatac cgaaaactca
    10861 gcctgaagga agggcgtact gatactgaag agcagcagtt acaggctatc cgtgatgtgc
    10921 ttcgccctca gcaggaggcc agaccggagg cacagccaca gccggaaaat gcggatgatg
    10981 gcgacgggag tatttacccg acggtgcggt tccgcgatcc ggatgaagtt cgtattgaaa
    11041 ttaacgggag tggtgcgtcc agaccagcag aacgcattga aaaggtgcgt ccggacaaca
    11101 gttatttcac ggatgaaaaa agtgctctgg ggagcgacgt tttcctcaat gccagcgcca
    11161 ccggcgtgaa accgtccgta gtgaagaaag gcgagaatca gtatgccgta gaaatggata
    11221 atcctgcgtt ctctgaagat gtggcaacgg agaccattaa cactctggct gacggagagc
    11281 gtattactga tgctgatccg atggaacagc ccgcgttcat gcgtgacccg cgattccgtg
    11341 gttttacggg ggatgataca gaagtgcagg cccgtcttgc ccgtggcaac gcgccgacgg
    11401 cggaagaact ggtgcgttcc cagatggctg aaggtgatgc cggtccgaca gcacaggagt
    11461 taactgagcg tccacgcctt cccgctcccg gcgatattca tcccggacag ggatatccgt
    11521 taccgggaga agtggcgcgt acgccggatg aaaatcaggc cggacgtggt ggtcgtttta
    11581 ccacaaccgg tgaggtgaag ggccagagtt tccagaaagg acgtgcgcag gcaccggaaa
    11641 atgccgctgg tcgccagggg gaaattctgg agggcgaacc ggttcgtcgt ggtctgccgt
    11701 caccggatga gcagaacgcc acagcaccag tgcgtgaagg acttcctgca cctgaaagtc
    11761 agcgtggggt tgatatgcca cagcctgaat cactccctcg tatagttcgt gactcccttc
    11821 cggaactggc acagcaggca gaagcacgca gacagaccgg ggataatcgc cagaccataa
    11881 ccgatgttcc ggatactgag gtgccggtgc cggtagataa accgagcact caccagcagg
    11941 cgcgtggtgc gaaaattgaa gactttggcg aggagattaa gggcgcggca aaacaccgtt
    12001 atgcgcagct tgctgaagct atgggtaaaa cgctggagga tggggagtac gcgacacaac
    12061 cactgagcaa actgtttcca aagccggatt atgcccgtct ggaaaaagaa ggtgtggata
    12121 gtgacacgct ggcaatgatg gcgttgtatc gcagtgaaat tcccaccaga acaagccgta
    12181 acatgcagaa atggatcagc attgttaaaa gcggtcgcga agctactgcc ggtatgctgg
    12241 aggggaaaat tccggcagcg aaactggctg atatgatgga caataagtcg gggttacgga
    12301 gcatgtcgga tacctggaaa ctgctccgta cgctttctgc atcgcagatt gataaagcat
    12361 ccggctatcg tgtcaggtcc ggtgtttatt cgtttgtcgg gggaaaacgt tacgatccgc
    12421 cgcaaatgat gtattcgctt cgtgatagca aggggcgcga cctctttttc tcggaaagcc
    12481 gggatgagtt actgaaaaag gcgaaagcat atttcgatga acaaggctcc agagaaaggg
    12541 aaacgccagc aacctctgct gatgacagaa taacctttga cgtgtaccgg cataaggcca
    12601 gtggcgacat ttttatcggt tatggcaaaa acaggcagaa actgaagggc ggttttgagt
    12661 ctgcccgtga tgcacatgat tatgtgcgca cacatcgtga cgagctggtt aatcaagtga
    12721 aggcgctgcg tgaagtctcg cgtgaggagc agcgtaacgc caccaaccgc gatcgtaccg
    12781 gaccagaacg ccgcaagggg gatgtttcgc cggagcagtt cagtgatgcg tttggttttc
    12841 gtggtgtgca gtttggtaat tatgtggaag gtccgcgtcg tcaggctgat ttgaaccggg
    12901 cttatgactc gctgcatgac ctggctgacg tgctgaacgt gccgacaaaa gcgctttctc
    12961 tgaacggtcg tcttgggctt gcttttggtg cccgtggtaa gggtaaggcg gcggcacact
    13021 atgagccggg tgaggtggca atcaacctga caaaaggtaa cggaccgggg gcgctggccc
    13081 acgaatggtt ccattcactg gataattatt ttggtcgtta tgatgtttcc actgacggga
    13141 agatcacgtc aggtggcgac tatatgacgg aagcacagcg tgccgggcgc gtatttaaag
    13201 acggcaggta tgttgatgcg gaatatccgg tacgtcagga ggtttacgac gcgtttaaag
    13261 gcgtgatgaa agccattaac agcagtgaca tgttgcgtcg ttctgaactc ctggatggcg
    13321 ctcgttcaaa accgtactgg tcaacggatg ttgaaatggc ggcgcgtgcc tttgagcgtt
    13381 atgttcagga taaagcgcgt atggctggcg tggagaatga ttatctggtc aatatccgta
    13441 aggcggatga ccatggacag ccggacacct acgcttatcc gacgaatgca gaactggatg
    13501 gcggtattcg tgaggcattc gatcacttgt tccgaacact gaaaacccgt gagacggaca
    13561 agggagttgc gttttattcc cgtaagggcg tgacccgcac acctgaaggc aatctgattt
    13621 cggatgttaa ccgcagtgcg gaagccaaag gcagtccggt cccgcaggtt gaagctgttg
    13681 cccgtggcgt gattagtggc attaaggaca gtgacctgaa ggtccgcgtg gtgaagtcac
    13741 agaaagaggc tgaagcactg gcgggtgaat cattcgacgg ttacggcaag gtgcacgcgt
    13801 tctatcgtcc ggataaacgt gaaattgtcc tggtggcgga taacatccct gacgggcgga
    13861 ccgttcgcga gaagttacgt cacgagatta ttcaccatgc aatggagcat gtagtcacgc
    13921 cagcggaata tcagacgatt attaagaccg tgctgaaaac ccgtgacagt gataacgcca
    13981 ccatccgtga agcctggcgt aaggttgatg cgtcttatgg taaggaatca ccggaagtgc
    14041 aggcgggtga atttctggcg catatggcag agaagcagcc gaataaattc gtggcggcat
    14101 gggtgcgtgt tgttgccctg gtcaaagggg tactgcgtcg tacggggtta ctgaagccga
    14161 cggaactgaa cgatatcaga cttgttcgcg aaactattcg cacgttaggc cagcgtgtgc
    14221 gggaaggtta cacgccgcgt gaggatggcg cggacgcatc gtctcagtac tcccgtagtg
    14281 gtaaacctga tccgttcaaa gtgccggaag gtgagggcga gcgttatcgt gatgaccttg
    14341 ccagaatgat gaagtcgtta cgttctggtg caatgactgc aaacatcggg cgtacgccgc
    14401 cggtattgcg ccaccttggc gctccagatt tgccgctggt tatctcccgc gacactgtgc
    14461 ggaaggctac caatggcgtg aaacatgtgg tgccaatgga tgttatcgag agacttccgg
    14521 aactgatgca caatccggat gcaatttacc gttccgcgac agaaagaaat gcggttgtga
    14581 tgctgcttga tgctgtggat aaaaatggtg atccggtcgt ctcggcagtg catatgaagg
    14641 cgacagataa acgtctggaa attaacaagg tggcatctgt ttacggtacg gaaaacggga
    14701 aaaagctgaa aagtatggaa atggctggat taacgcttta ccggaaggag aaattaagcc
    14761 gcgataaccc tcagtacagt gggctccaat tgcccaaaga ggagcgttct tatcgcggct
    14821 ctgtagataa aatactctat cctgaagata ttcgcaaggg gccgtattac tcccgtacca
    14881 gcagtctgac gccggaagag acaattgcat cgcgttttgt gcgccagatg caggataaat
    14941 tccaggtgct gaaagctgtt caggagaata tccgtaaaac tggcggaaaa atagacgaca
    15001 gtaacaacgc ttatatggcg gaagaactct tccacgggaa ggcggaaaac gacctgaacg
    15061 tgatgaagga gcgttacgtt cagccactgg ttaaattact ggcggactac aaaattgcgc
    15121 aggccgatct ggatgagtac ctctacgccc gtcacgcacc ggaacgtaac gcgcatatcg
    15181 cgaaaatcaa cccgaaaatg ccggacggcg gttcggggat gaccaacgcg gaggcggcgg
    15241 aaatcatgca gcgtgtgcgt aacagcggca aacaggcaca gtatgaccgt ctggcaggga
    15301 ttgttgacga tatgctggcc cgtcgccgtg agcttatccg tgaggccgga ctggaagaga
    15361 gcggcgtggt ggatgcctgg cagaaagcct accgttacta cgttcccctg aaagggcagg
    15421 atgttgacgg tgtggtgtca ctgccccgta caggtaaggg cttcaccatc ggcgggcgtg
    15481 aaagtaagca ggccatgggg cgtgcatccc gcgcacagtc tccttccact caggcgatac
    15541 aggatttgag tgaatcgctg atccgcaatc gcaaaaacga agtgggtaac gccttcctga
    15601 aactggtgca ggataatccc gataaggatt actggcaggt attcactgat gacagaccgg
    15661 ataccatgcg tgcgattgca gagcgcaagg accaggaaac tggtgaaacc attcgcgaag
    15721 ttgtcgagcg tccggtggcg atggcaatga tggcagaccg gtacttcacc accaaaaaga
    15781 acggcaaaac ttactacatc aaactccatg acccgcgcct gatgcgtgcg atgaagaaca
    15841 tggggccgga aaccagtaac gccgtaatcc gtacgctggg gaaagttaac cgcttcctgg
    15901 caacggtgaa cacgtcgtat aacccggaat ttctggtcag taacttcatc cgtgacgtgc
    15961 agacggcggt gatgaacctg aaggcggagc agggaaggag cgacggcaaa ctgaaagggc
    16021 tggataactt atccgccctg gctgtggtga aagacagccg ttccgccatg tcagccgtat
    16081 acgccagtct gcggggtaaa accctcacgg gcaaaggcgc acagtggcag aaggtgtgga
    16141 aagagtttgt tgaggatgga ggtaaaaccg gctggtttaa catgggtgac cttgaaggcc
    16201 agcagaagga aatggatcgc cttgtatcgc tggcgaaggg gggatggaaa ggccagagta
    16261 tcggtgcatg gaattcgttc ctgaaccttg tggaggatgc caacggtgcg gttgaaaacg
    16321 cactgcgtct ttctgcctat aagcacgccc gtgatgccgg tttgtcacgc cagcaggcgg
    16381 cgtctcttgc caaaaacatg acggtgaact ttaaccgtcg tggtgagcag ggggcgctga
    16441 tgaactcgct gtacatgttc gctaacgcca gcattcaggg gacggcaaac cttgtgagaa
    16501 cccttgggca ccttaatggt gacggtccgt tactggagcg ccttcgctgg aagaatctga
    16561 atgtacctca gaaaatcgcg cttgctgctg tgggagcggg ttatctgctt ggctcgctta
    16621 atcggagtgt ggcgggtgag gatgatgacg gggttaactg gtatgacaag gtgcctgatc
    16681 atgtgaaaga gcgcaacctc gttattatga aatcgatgtt cgggggcaaa gccggtgagt
    16741 actggagtat tcctctgcct tacgggtaca acgttttctt cctgctcggg cataccgctg
    16801 aaggtgtggc ggcgggtgac ctgacggcgt cccgtgctgc cggtaatgtt gttggtggtg
    16861 tgcttggtgc attcagcccg attggcagtg agacgtcgga aacactgtcc ggggcattgc
    16921 tgaaaaatgc agcgccgacc attctgcgtc cgtttgcgaa ccttgccatg aatgaaaact
    16981 tcatgggggc gcagatttac caggagaaca tgccgtttgg tacaccaaaa cctgacagcc
    17041 agctgggaag acgttcaacg ccagaagcgt acaaggcgtt tgcatcctgg ctgaatgcgt
    17101 tctcaggtgg cagccagtac cgtcccggcg cggtggatat cacaccggaa tcgctgaaat
    17161 tctggattga ctatatctcc ggagggacag ggcgcttcat ttccaaaacc acggatgcgg
    17221 cggtgaaatc gctgaatggt attgatatac cggaacagca ggtgcccttc ctggggaaaa
    17281 tttcggggga ggtgatgccg tatgcagacc agcagaagat gtatgaccgg atgacggagg
    17341 ttgcgcagta ccacgcagag ctgaagagtc tgagaggctc tgaaagaacg gcgttcattg
    17401 acgagaacaa cggaaaattg tcgatgaacg ggcttatgca ggatacccgg aagagactga
    17461 aggatttgcg taaacagcgt gatgccattt acgccgacag tactctcagt ctggcgcaac
    17521 agtcggcgat ggtgaaatcg gtagagcggg atatgaaaat tgccgtggat cggtttaacc
    17581 gcgagtacaa caaaaaagtg ggagtggagt aaagaacatg ccccgtacgg aagtgcgggg
    17641 catgcttaac tggatgtgta cataaatgag tacagttatg acgagtattt tggtatttgt
    17701 tatatttaaa tcaatggatt aatgattatg ttcattatat ccatttaact aagaggacaa
    17761 tgcggcatga gtatacccgc taatggactg cggggtaagt acgctgccgc tcgattgctt
    17821 aaaccctcgc catttatgct gggtttttat catttttctt aatgttttcc gcacgttctg
    17881 ctttttggcg tgcttctgct ttacgcttgt tgctcatgtc gttacgaatc tgtgcatgac
    17941 tcattaacgc gaagataaag gtgccgccgc agatgttccc cgctaaagta ggtagtgcga
    18001 agggccagat gaaatcgctc cagtgcagcg tgccgttaaa caccagatag aggatttcaa
    18061 cagaaccgac aacgatgtgg gtggtgtcac ccagtgcaat aagccaggtc atcaaaataa
    18121 tcaccacaat ctttgccgca cccgctgcag gaaacatcca aaccatagtg gcgatcagcc
    18181 agccggaaat gatcgcgttg gcaaacatct cgctgggggt gttcttcatc acatccatgc
    18241 cgattttgac aaatgcatcg cgagtttctt cattgaagat aggcatatat tcaaatgccc
    18301 atgcagcaat acctgtcccg agaatattac ccagcagcac gacgccccat aatcgcataa
    18361 gtaagccgac gttgctcatt gtcggttttt gcatgacggg tagtaccgca gtcacggtgt
    18421 tttcggtaaa taattgctgg cgggccataa tgacgataat aaaaccaaag gtataaccga
    18481 gattctccag taagaagctg cctggcactc cttccagttc gacatgaaat atcccttttg
    18541 ccagtagcga agcgcccatc gacagacccg ccgcaatggc tgaccacagt agcgccattg
    18601 cgtcgcgttc cagctctttt tcaccatcct ggcggatatg ctcatgaatt gccatcgccc
    18661 gggaggggag tcggtcttca tctatttcta tttttttgcc gcgctctttt tcttcgctct
    18721 caacttcaat ttcgtcgctg tgttgatcaa ttttgtcgtt gtccatggtc tcttctcttg
    18781 aattagcacg tatagctaaa gcgtagcggc ttttttgctc gcaactggcg ggagttactc
    18841 tgaaaatgta gaaaaggctg cgtttgcctt tttctgtttc tatagaatca agtagcctac
    18901 agggcggcga ttaccaggct atgatcaaat cagcaaatca gggcgtctgg acatcagttg
    18961 acgtgctgtt acaatcgccc acacctaaac aggcggatac ggtatcgttc cgtcatggat
    19021 ggcaaactgc ataagccata aaaaaacagg gagacattta tgaagcttcg cctgtcggcg
    19081 cttgctctgg gaactacgct tctggtgggg tgtgcgagtt ccggtacaga tcagcaaggg
    19141 cgttctgacc cgttagaagg gttcaaccgc accatgtaca acttcaactt caacgtatta
    19201 gacccgtata ttgttcgacc ggtcgctgtc gcctggcgtg attatgttcc gcaaccggcg
    19261 cgtaatggtt tgagcaactt tactggcaac cttgaagaac ctgcggtgat ggttaactac
    19321 ttcttgcagg gcgaccctta tcaggggatg gtccacttta cccgcttttt cctgaacacc
    19381 attttgggga tgggcggctt tattgatgtt gcagggatgg cgaacccgaa actgcaacgg
    19441 actgaacctc accgcttcgg tagtacgctt ggtcattatg gcgtgggtta tgggccttac
    19501 gttcagttac cgttctacgg tagcttcacg ctgcgtgatg acggtggtga tatggcggat
    19561 ggtctttacc cggttctttc ctggctgacc tggccgatgt ctgtgggtaa atggacgctt
    19621 gaagggatcg aaacccgcgc tcagctgctg gattccgatg gtctgctgcg tcagtcgtcc
    19681 gatccttata ttatggtgcg cgaagcgtac ttccagcgtc atgatttcat cgctaatggc
    19741 ggcgaactca aaccgcagga aaatccgaac gcacaagcga ttcaggatga tttaaaagat
    19801 attgattctg aataagaaac aaataaaaaa ggtgagtcgc aaaactcacc ttttttgttg
    19861 ttatccctca attaccacaa gtaaaaggag ataaaccgac aatgacccgg acgtaatgat
    19921 gtaaaaatac tgatgttcgt cactgactat ttccgatatt caggcgttga aaaatcaaaa
    19981 aactggaaaa ataattttaa atatcatcaa aattaacacc tggcgcagat agttgattaa
    20041 tttcattttg atatttcgcc tggcgatttt gaagtctttt ttcaacgtta tcttcgagat
    20101 ccttaaagtc tttgtgttta ttttcaggat ggtcaacgtc aactttgtta gcgaataaat
    20161 tagaaattat ttcttttaca ttgtcgcgtc taaattttgc atcgtccact tgaaaattat
    20221 atcttattgc tcgacctaaa cgtttaaaaa cttcgttagg ttctctcagt ctttccccgt
    20281 tgagctcgtt tacaacctga tgatcggaac ctgttgcaaa tgattctgcc atgatagctt
    20341 tactgctttc aaaccacgct gaagcattcc tggctttttc actacaatat tgggtgagac
    20401 tatcaccatt aatacgaatt cctctttgaa tgtggtagtc gtcctgatcg tagtaaccga
    20461 gagataagta atcatttccg tttttatttt taattacaat agcgagagag tcgccttttt
    20521 tatctttacc gtaccaggta aataagtccg ttggttcgct attcccgctc tcatcaaact
    20581 ccgttaccgt atgtctgagt ttgtgtgata tcttatcgaa gataaaacca ggatctccat
    20641 catatatttc ttttaatgcg ctgaagatca tttccttgtt attgatatca tgagtcttct
    20701 caactatgtt gtcattaatg atagtacgga cgagttcttt gtctttatag ttcgcaggta
    20761 attctattgg ttcttggttg tcgataccaa tagtaatatg gtctgtatca ttggagccaa
    20821 tatcaatata agtaactgag gaattattaa tattaaatct ataaggatcg tcgtttactc
    20881 gtatggcgtt ttttataagc tcaagtagat gtttaataca ggtgcttatt ccttccttac
    20941 cattaaaaag gtttttgagc tcttgtacta atccattaac ttcatcattg ttattttcat
    21001 taatggattc tgggtaagtt gcgctggtat tgttgattga aatagatggt agcatgattt
    21061 tattcca
//
LOCUS       DAZUFB010000086        21012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZUFB010000086 DAZUFB010000000
VERSION     DAZUFB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.086"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..713
                     /locus_tag="ACHY3M_003498"
     CDS             join(16..265,265..713)
                     /locus_tag="ACHY3M_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HFS4318339.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(729..1370)
                     /locus_tag="ACHY3M_003499"
                     /pseudo
     CDS             complement(729..1370)
                     /locus_tag="ACHY3M_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680802.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(1395..3068)
                     /locus_tag="ACHY3M_003500"
     CDS             complement(1395..3068)
                     /locus_tag="ACHY3M_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFS4318340.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(3124..3435)
                     /locus_tag="ACHY3M_003501"
     CDS             complement(3124..3435)
                     /locus_tag="ACHY3M_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4318341.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(3463..4785)
                     /locus_tag="ACHY3M_003502"
     CDS             complement(3463..4785)
                     /locus_tag="ACHY3M_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4318342.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(4900..5211)
                     /locus_tag="ACHY3M_003503"
     CDS             complement(4900..5211)
                     /locus_tag="ACHY3M_003503"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4318343.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            5410..6108
                     /locus_tag="ACHY3M_003504"
     CDS             5410..6108
                     /locus_tag="ACHY3M_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4318344.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(6153..7052)
                     /gene="eamA"
                     /locus_tag="ACHY3M_003505"
     CDS             complement(6153..7052)
                     /gene="eamA"
                     /locus_tag="ACHY3M_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFS4318345.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            7247..8434
                     /gene="ydeE"
                     /locus_tag="ACHY3M_003506"
     CDS             7247..8434
                     /gene="ydeE"
                     /locus_tag="ACHY3M_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFS4318346.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFTDNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGHRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            8561..8656
                     /gene="mgtS"
                     /locus_tag="ACHY3M_003507"
     CDS             8561..8656
                     /gene="mgtS"
                     /locus_tag="ACHY3M_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFS4318347.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            8656..8760
                     /gene="mgtT"
                     /locus_tag="ACHY3M_003508"
     CDS             8656..8760
                     /gene="mgtT"
                     /locus_tag="ACHY3M_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFS4318348.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(8875..9765)
                     /gene="dgcZ"
                     /locus_tag="ACHY3M_003509"
     CDS             complement(8875..9765)
                     /gene="dgcZ"
                     /locus_tag="ACHY3M_003509"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFS4318349.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(10020..10412)
                     /gene="ydeI"
                     /locus_tag="ACHY3M_003510"
     CDS             complement(10020..10412)
                     /gene="ydeI"
                     /locus_tag="ACHY3M_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFS4318350.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            10688..11206
                     /gene="ydeJ"
                     /locus_tag="ACHY3M_003511"
     CDS             10688..11206
                     /gene="ydeJ"
                     /locus_tag="ACHY3M_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFS4318351.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(11251..13296)
                     /gene="dcp"
                     /locus_tag="ACHY3M_003512"
     CDS             complement(11251..13296)
                     /gene="dcp"
                     /locus_tag="ACHY3M_003512"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFS4318352.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            13433..14179
                     /gene="ydfG"
                     /locus_tag="ACHY3M_003513"
     CDS             13433..14179
                     /gene="ydfG"
                     /locus_tag="ACHY3M_003513"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFS4318353.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            14268..14954
                     /gene="rspR"
                     /locus_tag="ACHY3M_003514"
                     /gene_synonym="ydfH"
     CDS             14268..14954
                     /gene="rspR"
                     /locus_tag="ACHY3M_003514"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFS4318354.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            15132..15335
                     /gene="ydfZ"
                     /locus_tag="ACHY3M_003515"
     CDS             15132..15335
                     /gene="ydfZ"
                     /locus_tag="ACHY3M_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFS4318355.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(15371..16831)
                     /gene="ydfI"
                     /locus_tag="ACHY3M_003516"
     CDS             complement(15371..16831)
                     /gene="ydfI"
                     /locus_tag="ACHY3M_003516"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4318356.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(16920..18203)
                     /gene="ydfJ"
                     /locus_tag="ACHY3M_003517"
                     /pseudo
     CDS             complement(16920..18203)
                     /gene="ydfJ"
                     /locus_tag="ACHY3M_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            18335..18577
                     /locus_tag="ACHY3M_003518"
     CDS             18335..18577
                     /locus_tag="ACHY3M_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4318357.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTRESADEWFVR"
     gene            complement(18705..18844)
                     /locus_tag="ACHY3M_003519"
                     /pseudo
     CDS             complement(18705..18844)
                     /locus_tag="ACHY3M_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            19173..19823
                     /gene="nleG7"
                     /locus_tag="ACHY3M_003520"
     CDS             19173..19823
                     /gene="nleG7"
                     /locus_tag="ACHY3M_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFS4318358.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(20048..20923)
                     /locus_tag="ACHY3M_003521"
     CDS             complement(20048..20923)
                     /locus_tag="ACHY3M_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFS4318359.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT         5817 a         5021 c         4461 g         5713 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
      241 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
      421 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
      481 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
      541 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
      601 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
      661 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
      721 tcattaccta ccgcctggta cattatttgt tcgcgctcat tgatatcttc ctctggcgaa
      781 tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac ctgattattg
      841 atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa gaccgttgat
      901 gcgaagcgga taaaaaagtc gatcagttta cggtttcgcc agccaccata ttttgtcacc
      961 agatgataag gcatctcaaa atgtgaaagc gtcaccacag gttccattcc ctgcttaagg
     1021 cattcatcga acagatcatc ataaaattgt aacccctctt cattcggctc ctgttcgtca
     1081 ccttgcggaa agattcgtgt ccagacaatg gaagttcgaa agcatttgaa tcccatctcg
     1141 gcaaataact gaatatctgt tttatagcga tgataaaaat caattgcttc atgattggga
     1201 taattaagcc cgtcgataac gccttctgtc acttcacgcg gcaccccgtg agcgccagca
     1261 gtcatcacat cagcgatgct gatgcctttt cctccttcat tccagccacc ttccaactga
     1321 tgcgcggcta ccgcaccgcc ccataaaaaa ccttttttaa atcctgacat acacaactcc
     1381 ttaactaaaa tagattacca ccagacttcc gcctggatgc cggccataaa ctcgtctttg
     1441 ctgttctcat taaacttaaa attcgataat tcgttatcca gaatattgat atacgttcca
     1501 tagaagcgaa tttctggtcg tgaacctaaa atgctttcac ccactttcaa tgcatgccag
     1561 agtgtcgttt tataagccga ttcattaagt gttacaccct gctgagtttt gttctgttgc
     1621 ttaaaccagc ctaattcaag ccccgtctga ttccatgtat tccagatcca ggccggtctt
     1681 attacagtgc gaatactgtc aaaatcacta tgagcaccac tttcataact ataaacatct
     1741 tcgccgtgag aatagacaag cgcgttagcc ataataatat tgtcagtaag atacatctcg
     1801 ccctgagaga ttaaacgcca ggcgatccca ttggtatgat caccatagta atagcgacca
     1861 tgcgccatcg tattactggc atctgagaaa ctggcaaaac tgctggcata ggaattattg
     1921 gcaacctgta acgtaaattc attaaacgta tcgcgttgca ggttttgccg taaaacagaa
     1981 gtcagcatcc agctatcttt catttcaaaa taactgttgt cattttcgtt atcttgttgt
     2041 tgatccgttt tattaggtgc ggaatattta gccattaatg ataatgtcgc atcatcccat
     2101 aacgggatat tgcgataacg aacatccaca gaattggtat tcatctggct ggtacgcgaa
     2161 aaatcacgag agtaaacatc gacatcatca cggcttaagg acatatcaaa cagccctaca
     2221 ccaagtgccc agttttcaat ccccacaccc gcggcgacat ccgtggttaa ggttttccag
     2281 tccagcatct ggatctcata ttgcgggagt ttatgtttgc cgacccagaa gtctgcctct
     2341 ggtgcgaagg gtaaaaaacc tcgcgttgtc aggtaaatat cgctgaactg catgatattt
     2401 tcattggcac tgtcaccaaa ccaggcatcg ttatactgct cacccacgtt gccatcatag
     2461 gtaacgaccg catttgccgt tttaccgtcc tgattataaa cacgctgatt taaggtcagg
     2521 tcaaaccaac cgctcatctc attaccaaaa cgtcccagag aacccgctgc ataagtttgt
     2581 ggtgagccgt gattaccagt accccagcct gagcggaagt acccctgata gctgaaccca
     2641 atatcatcct ttatatattt actgatatct ttcagggtca cgctttcgat agtggttttg
     2701 tcgcctttac tttcaataac ggcaacttgc tgcgatccat ttatagtatg cgctgcggta
     2761 aacgagttct gttcaccgtt ttcatttttg atgttatcag cgacgggaga tgtcgctgat
     2821 atttcagcaa gcgaggctga tttatatttg ttttccgtga tgcttttttg taaggtcgaa
     2881 agtcgggatt tatatactcc cagttcattc tgtgttgctt tcagctcttg tttattttgc
     2941 gacaattcat tttcaagcaa ttcaaggcgc tgctctatgg ttaattttgt tgccatcgcg
     3001 ttcaatggaa acaatgctgt aataatagaa aaagacaaaa ggctgacgtt aagcgtcttt
     3061 atattcatga cttatcctta agagtataat aatttcctcc agctatcgca ctggaggaaa
     3121 aaatcaggca tggagttctt ttcttaatga aataatttcc ctggctaaat cctggcataa
     3181 tattgcattc atcaaatgat cctgtgcatg aatcagaatt aaattaactt ttattttgcc
     3241 acagccttca tcctgactaa ttaatgcggt ctgaatctta tgtgcctgaa gacaggcaga
     3301 ttcagaactg gccattagct gagttgcgcc ctgccagtct ttttgcccgg cacaacgtat
     3361 ggcttccatc gcgtcagaac gtgcctgccc cgcgttgacc agtaactcca taacaagttc
     3421 ttcatctgca aacatcacaa tctccttcct gggtaaatgg aattatccgt tagttgtcat
     3481 gttattgact ttgttggcgg caataacaaa aggtaaatag atcattgtcg ccacacctaa
     3541 acataaaaaa ccaaccagta aagccagcca gttccccccg gttccgagga aagcaattaa
     3601 aggtccgggc gtagtccagg gcacggcata agcaaccggt ggaataattt ccatcgaaac
     3661 aaagaagtaa ccaatggcac agttaacaat gggaaccata ataaacggca ccatcatgat
     3721 ggggttaagt acaataggaa ggccaaacat tatcggttca ttgatattga aaataccggg
     3781 gataaatgac atttttgcca gatcacggta ttccgcacgc ttagaagcga taaaaatagc
     3841 caacaataac cccaacgtca tacctgaacc gccgcagttg gcgaatgcat cattaataga
     3901 agtccaggta atcggatatg gcgcgcccca ggtagtgcca tgttgagcgg cccaggagag
     3961 attctccaga ttagcctcag aaaaaacggt ttctcgaatt gccgatgtgg tattaggtcc
     4021 gtggatcccc agcacccaca ggaaattacc cacagccccc aggataatta ccgcaaagat
     4081 attcgttccc atatgtttga gcggcgtctg gattaatgtg taaataagat cgtttagtcc
     4141 tgccggggat atcagtgtca ggcaataatt aagtgcggaa aagaacacca tgacaaaaaa
     4201 tattggcaat aaaactttaa atgaacgcgc tactgcaggc ggtacagctg ccggcatcgt
     4261 tatggtgatt tttgggtttc gagctaagcg acaaaatatt tcactggtga tcaatgcaac
     4321 gataacagca acaaacaacc cctgcgcgcc gagatatttg gtcgtcagga atgcttgccc
     4381 atctatcatc tgatatggcg tataaacaat aaaaaatgct cctattgccg ttaatccgca
     4441 taaaagatca tcttgctcat agctaatcgc catattcctg gcgaccagga aagcaatcat
     4501 aattgacatg atatttacag aaccattcat taccggagta aaaatagctt gtgctttttc
     4561 aaggttgggg aaaatgctat tcagatgcag taatccggca ataaaaccgt ctggcgataa
     4621 tatggcaaag ttaattaata taattaatga acttgccatg ataattggaa acgtaaaaac
     4681 gaatccatct ctcaccgctg caacatgttt ttgtgagttt aacttgatag caactggaac
     4741 aagaaaacgt tccattccac gttcgaatga ggccattaat cccatttttt tatacctgtt
     4801 gttttggata tttatacatc ccaataaagc atgtatgcca tatccatttt atgatgtgtg
     4861 aaaagttagt tcaggtaata gtttaataat attagattct tactcttgca gtagtttcat
     4921 cgcaaattca agaactgcct gtccgttcat ggtgccgtag tctttcatct cgataacggc
     4981 tacaggcatt ttcccctgtg caacggcttc aatctctggt ttctgaaagc gaacttgcgg
     5041 gccaagtaaa acaacgtcaa cttcgttatt tttaattttc cctttagcct ctgcaatcgc
     5101 caatgcggaa atattaactt caagtgaaat agcgttagca tgatcaatca tacgtttaac
     5161 cagcatactg gttgacatgc ccgcagcgca aactaacaat atttttttca tattttccct
     5221 tactggtata taacagatta cattgtttaa atatcatatc ccgcagtagc tatacgtcct
     5281 ggtacattga tcacagtaat acagtgataa agttgttcaa catcagggtg attattaaac
     5341 gactcggcca tacgataaac caatcaggac tcaactcctt gttttcaatg gctcttaaaa
     5401 ggtgcaaaag tgatttttca aaagattgcc cgcttactta aatccgaaat caatggcaat
     5461 tcatggcatg ttggtgattt gttgccgtca gaagcggaac tcgctgttcg ttataatgtt
     5521 tcgcgtaata ccctgcgtaa ggcattatcc ttgctggaag gcgaaggaat tattcacaga
     5581 aagcatggtt caggaagata cattcaaaaa aagaattttg ttgcacacat tgatcacatg
     5641 aacagtttca gtgaaattgc gcataaaagc ggcaaagagg cagggagtca gattatgaaa
     5701 tttgaagtgc aggatgcttc acccactatt gcgactgagc tgaatttagt gactggtgag
     5761 caggtttatt acataaagag actgcgattc attgaggata atgcagcaca attagaagaa
     5821 acgtggatgt cagtagcacg ttttcctgat ttaaccgtgt cacatatgca aaaatccaaa
     5881 ttttcatata ttgagaatga atgcgggatc aaaatcattg gcacctttga aactttctcc
     5941 cctacttttc ctaccccaga aatcgccagt attttacgga tcagcccacg ggatcccata
     6001 cttaaaattc agacccaggc tgtggacagt aactctattc cgctggatta ttcgttactt
     6061 tacagcaata ttttcgagtt ccaggtaaag tactttttcc cgcgataaaa gcagacgcac
     6121 agagtgctga catcacattc tgtgcgtatg gtttagcctc taacctttac cgctttacgc
     6181 caccgcaagc caaatacatt gatatacagc ccggtcataa tgagcaccgc acctaaaaat
     6241 tgcagacccg ttaagcgttc atccaacaat agtgccgcac ttgccagtcc gactacgggc
     6301 accagtaacg ataacggtgc aaccctccag gtttcatagc gtcccagtaa cgtcccccag
     6361 atcccataac caacaattgt cgccacaaac gccagataca tcagcgacaa gatggtggtc
     6421 atatcaatag taaccagact gtgaatcatg gatgcggaac catcgagaat cagcgaggca
     6481 acaaagaagg gaatgattgg gattaaagcg ctccagatta ccagcgacat caccgccgga
     6541 cgcgttgagt gcgacatgat ctttttattg aagatgttgc cacacgccca actaaatgct
     6601 gcagccaggg tcaacataaa gccgagcatc gccacatgct gaccgttcag actatcttcg
     6661 attaacacca gtacgccaaa aatcgctaag gcgatccccg ccaattgttt gccatgcagt
     6721 cgctccccga aagtaaacgc gccaagcacg atagtaaaaa acgcctgtgc ctgtaacacc
     6781 agcgaagcca gtccagcagg cataccaaag ttaatggcgc agaacagaaa ggcaaactgc
     6841 gcaaaactga tggttaatcc ataccccaag agcaaattca gtggtacttt cggtcgtgca
     6901 acaaaaaaga tggccggaaa agcgaccagc ataaagcgca aaccggccag catcagcggt
     6961 ggcatgttat gaagccccac tttgatgacc acaaaattta gcccccatac gaccactacc
     7021 agtagcgcca acaccccatc ttttcgcgac attctaccgc ctctgaattt catcttttgt
     7081 aagcaatcaa cttagctgaa tttacttttc tttaacagtt gattcgttag tcgccggtta
     7141 cgacggcatt aatgcgcaaa taagtcgcta tacttcggat ttttgccatg ctatttcttt
     7201 acatctctaa aacaaaacat aacgaaacgc actgccggac agacaaatga acttatccct
     7261 acgacgctct accagcgccc ttcttgcctc gtcgttgtta ttaaccatcg gacgcggcgc
     7321 tacgctgcca tttatgacca tttacttgag tcgccagtac agcctgagtg tcgatctaat
     7381 cggttatgcg atgacaattg cgctcactat tggcgtcgtt tttagcctcg gttttggtat
     7441 cctggcggat aagttcgaca agaaacgcta tatgttactg gcaattaccg ccttcgccag
     7501 cggttttatt gccattcctt tagtgaataa cgtgacgctg gtggtgctct tttttgccct
     7561 tattaactgc gcctattccg tttttgctac cgtgttgaaa gcctggttta ccgacaatct
     7621 ttcgtccacc agcaaaacga aaatcttctc aatcaactac accatgctaa acattggctg
     7681 gaccatcggt ccgccgctcg gcacgctgtt ggtgatgcag agcatcaatc tgcccttttg
     7741 gctggcagct atctgttccg cgtttcccat gcttttcatt caaatttggg taaagcgcag
     7801 cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg ccgaaagttt tattacaaga
     7861 taaagcactg ttgtggttta cctgctctgg ttttctggct tcctttgtta gcggcgcatt
     7921 tgcttcatgc atttcacaat atgtgatggt gattgctgat ggggattttg ccgaaaaggt
     7981 ggtcgcggtt gttcttccgg ttaatgctgc catggtagtt acgttgcaat attccgtggg
     8041 ccaccgcctt aacccggtta acatccgcgc gctgatgaca gcaggcaccc tctgtttcgt
     8101 catcggtctg gttggtttta ttttttccgg caacagcctg ctattgtggg ggatgtcagc
     8161 tgcggtattt actgtcggtg aaatcattta tgcgccgggc gagtatatgt tgattgacca
     8221 tattgcgccg ccaggaatga aagccagcta tttttctgcc cagtctttag gctggcttgg
     8281 tgctgcaatt aacccattag tgagtggcgt agtgctaacc agcctgccgc cttcctcgct
     8341 gtttatcatc ttagcgttgg tgatcattgc tgcgtgggtg ctgatgttaa aagggattcg
     8401 tgcaaaaccg tgggggcagc ccgcgctttg ttgatttaag tcgaacacaa taaaaattta
     8461 attcagcctt cgtttaggtt acctctgcta atatctttct cattgagatg aaaattaagg
     8521 taagcgagga aacacaccac accataaacg gaggcaaata atgctgggta atatgaatgt
     8581 ttttatggcc gtactgggaa taattttatt ttctggtttt ctggccgcgt atttcagcca
     8641 caaatgggat gactaatgaa cggagataat ccctcaccta accggcccct tgttacagtt
     8701 gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga
     8761 atgcttgcat ggatagattt gtgttttgct tttacgctaa caggcatttt cctgcactga
     8821 taacggatcg ttgacacagt agcatcagtt ttctcaatga atgttaaacg gagcttaaac
     8881 tcggttaatc acattttgtt cgtcaataaa catgcagcga tttcttccgg tttgcttacc
     8941 ctcatacatt gcccggtctg ctcttccaat gaccacatcc agaggctctt caggaaatgc
     9001 gcgactcaca cctgctgtca cggtaatgtt gatatgccct tcagaatgtg tgatggcatg
     9061 gttatcgact aactggcaaa ttctgacacc tgcacgacat gcttcttcat cattagccgc
     9121 tttgacaata atgataaatt cttcgccccc gtagcgataa accgtttcgt aatcacgcgt
     9181 ccaactggct aagtaagttg ccagggtgcg taatactaca tcgccgatta aatgcccgta
     9241 ggtatcatta accaatttaa atcggtcaat atccaacaac attaaataaa gattcagagg
     9301 ctcagtgttg cgtaactgat gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc
     9361 cgtcaaaaca tccatattgc tacggatcgt cagcaaataa attttgtaat cggttaatgc
     9421 cgcagtaaaa gaaagcaccc cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt
     9481 ttcaacaata gccagcatta attcccgacc acagttatgc atatgttgat gggcagaatc
     9541 cattagccga acgtaaggta attcatcgtt atcgagtggc cccagatgat caatccaccg
     9601 accaaactgg cacagtccat aagaatggtt atccgttatt tctggcttac tggcatctct
     9661 cgcgaccacg ctgtgaaaca tactcaccag ccactggtag tgggcatcga tagccttatt
     9721 gagatttaac aagatggcat caatttccgt tgttttcttg atcattgcca ctcctttttc
     9781 acagttcctt gtgcgcgcta ttctaacgag agaaaagcaa aattacgtca atattttcat
     9841 agaaatccga agttatgagt catctctgag ataacattgt gatttaaaac aaaatcagcg
     9901 gataaaaaag tgtttaattc tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca
     9961 aatagcataa cccaataccc taatggccca gtagttcagg ccatcaggct aatttatttt
    10021 tatttctgca aatgagtgac ccgaacgacg gccggcgcgc ttttcttatc cagactgccg
    10081 ctaatgttga tcatctggtc cggctgaact tctcgtccat caaagacggc cgcaggaata
    10141 acgacattaa tttcaccgct cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa
    10201 atcaaattac cgcgtagtga aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg
    10261 gtcatttttt ttgcatcatc ggttccgcga tagccatctt ctattgcatg aggcggcggt
    10321 ggcgctgcat cctgttttaa accgccctgg tcatctgcca acgcataagg catgacaaga
    10381 aaacttgcta atacaatggc ctgaaatttc atactaactc cttaattgcg tttggtttga
    10441 cttattaagt ctggttgcta tttttataat tgccaaataa gaatactgcc aattgttata
    10501 aggcatttaa aatcagccca ctagctgtca aatattcaga gaatttaact cactaaagtt
    10561 aagaagattg aagagtctta aacatatttt cagaataatc ggatttatat gtttgaaaat
    10621 tattatattt gacgagcata cagaaaaagc aaatcacctt tacatataat agcgtggaca
    10681 aaaaacagtg aacattaata aagataaaat tgtacaactc gcagataccg acactattga
    10741 aaacctgaca tccgcgttga gtcaaagact tatcgcggat caattacgct taactaccgc
    10801 cgaatcatgc accggcggta agttggctag cgccctgtgt gcagctgaag atacacccaa
    10861 attttacggt gcaggctttg ttactttcac cgatcaggca aagatgaaaa tcctcagcgt
    10921 aagccagcaa tctcttgaac gatattctgc ggtgagtgag aaagtggcag cagaaatggc
    10981 aaccggagcc atagagcgtg cggatgctga tgtaagtatt gccattaccg gctacggcgg
    11041 accggagggc ggtgaagatg gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa
    11101 aggccagacc tatactgcgg ttatgcattt tgctggcgac tgcgaaacgg tattagcttt
    11161 agcggtgagg tttgccctcg cccagctgct gcaattactg ctataaccag gctggcctgg
    11221 cgatatctcc aggacagcca ttggtggtgt ttatatgttc aagccacgat gttgcagcat
    11281 cggcataatc tgaggagcct taccgcgcca ttgtcgatac aggcgttcca gatcttcgct
    11341 gttacctctg gaaaggatcg cctcgcgaaa acgcagccca ttttcacgcg ttaatccgcc
    11401 ctgctcaaca aaccactgat aaccatcatc ggccaacatt tgtgtccaca gataagcgta
    11461 ataacctgcg gcatatccgc caccaaaaat atgggcgaaa taactgctgc gatagcgtgg
    11521 cggtatagca ggaagatcca tattttccgc caccagcgcc cgcaattcaa aatcatcgac
    11581 atcctgcatt gcttcgtttt cttccaggca atgccagcgc atatcgagaa gtgcggcgct
    11641 aagcagttcg ctcatctcat accctttgtt gaacaggctg gcattacgca ttttctgttg
    11701 cagttcgtca ggcattgctg ccccgctctg ataatgccgg gcgtagcgag cgaatacctg
    11761 cggatgcgtt gcccagtgtt cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt
    11821 gttggtgccg gaaagcgtgg cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc
    11881 aaattcatgg aataaggtta tgacatcatc ccagagtaac aacgcaggct caccggcagc
    11941 gggtttctga taattgcaaa cgttataaat taccgaatgc gtttcattaa gcgttgattg
    12001 ctcaacaaaa ttgcccatcc atgcaccgcc gctttttgaa tcacgggcga agaaatcacc
    12061 gtaaaataac gccagtccca cgccattatg atcaaaaatt tcccacacac gaacgtcagg
    12121 atggtagaca ggaatatcaa aacgttcgac aaacttaata ccgaagagct gattcgcggt
    12181 ccagaataca ccttcattca acaccgtgtt taattcaaaa tatggcttga gctgcgcctc
    12241 atcaagatca aatttctccc gccgtacctg ttcggcataa aatgcccagt cccacggctg
    12301 cgcgctaaac ccgccctgct gcttatcaat aaccgcctgt atggaggcta attcatcgct
    12361 cgcacgttga cgcgccgctg gaacaatttc ccgcataaag ttgagcgctg cttcaggtgt
    12421 ttttgccatc tgatcggcga ttttccatgc ggcataatga ggaaaaccaa gtaatgtcgc
    12481 ctgttgtgca cggatctcca ccagacgttg aatgatagcg cgggtatcat tgccatcatt
    12541 tttttccgct cgcgtccagc ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat
    12601 ttcggcaagc gccggttgtt gggtggtatt cagcagcgga atcagccatt tgttatccag
    12661 acctttctcg cgagccgcct ctgccgccag cgcaatctct tgctcactca ttcctgccag
    12721 ctgcgcgata tcgttcacaa ccagaccgcc ggatttattt gctgccagta accgctggtt
    12781 aaactgactg gtcagtgtcg cagcttctgt attcagtact tttaattttg ctttatcagc
    12841 ttgcgcaagt ttggctccgg caaggacaaa acgttgatga atcacctcca ccaggcggat
    12901 ggattcacta tcaagcccca gggattcacg gcgctgccag acagcatcta cccgcgcgaa
    12961 taattcaccg ttcagataga tatcattagc cagttccgcc agttcagcgg aaaactgttc
    13021 atcaagacgc tgtaattcat cattggtatg cgccgcagtc atcgcaaaaa agacgctggt
    13081 aacgcgggta agtaattctc cgctttgttc cagtgccaga atagtattgt tgaaatcagg
    13141 cgtttgcggg ttaagcgcga tggcagcaat ttctgcccgc ttttgctgca ttccctcatc
    13201 gaatgccggg cgatagtgat gattggcaat ttgatcaaaa tgaggagcca gatacggcag
    13261 tgtgctttgc acaaggaaag gattcattgt tgtcattttc ttctcctgaa cgcgaggtgt
    13321 tccatagcgt aggcttactg ataacggagt gcaatcttgc aaaccagtat tacccgctct
    13381 taagcatcac gtgctatgtt attgacacac aaaagcgttg aggaacagtg agatgatcgt
    13441 tttagtaact ggagcaacgg caggttttgg tgaatgcatt actcgtcgtt ttattcaaca
    13501 agggcataaa gttatcgcca ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga
    13561 actgggagat aatctgtata tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga
    13621 gatgctggca tcgctccctg ccgagtggtg caatattgat atcctggtaa ataatgccgg
    13681 cctggcgttg ggcatggagc ctgcgcataa agccagcgtt gaagactggg aaacgatgat
    13741 tgataccaac aacaaaggcc tggtatatat gacgcgcgcc gtcttaccgg gtatggttga
    13801 acgtaatcat ggtcatatta ttaacattgg ctcaacggca ggtagctggc cttatgccgg
    13861 tggtaacgtt tacggtgcga cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac
    13921 ggacctgcat ggtacggcgg tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac
    13981 cgagttttcc aatgtccgct ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa
    14041 taccgttgca ttgacgccag aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc
    14101 tgctcacgtc aatatcaata ccctggaaat gatgccggtt acccaaagct atgccggact
    14161 gaatgtccac cgtcagtaat ttttataccc ggcgtaactg ccgggttatt gcttgtcaca
    14221 aaaaagtggt agactcatgc agttaactca ctcacaagca agaacgaatg accgtcgaaa
    14281 cgcaacttaa tcccacacag cctgtcaatc agcagattta tcgtattctt cgtcgcgaca
    14341 ttgtccattg cctgattgca ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt
    14401 tcaatgtgtc acgccagccg gttcgtgaag cctttattaa actggcggaa aacggcctga
    14461 ttcaaattcg tccgcagcga ggcagctacg tcaacaaaat ttccatggcc caggtgcgaa
    14521 acggcagttt tatccgtcag gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga
    14581 ttaccgaaag ccagtgctac caactggaac aaaatcttca ccagcaacgc attgccattg
    14641 agcgcaagca actggatgat ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc
    14701 agattgccga ctgtcaactg gcgtgggata ccattgagaa cctgaaagcg accgttgatc
    14761 gcgtgcgcta tatgagtttc gaccatgttt ctccaccaga aatgctgtta cgccagcatc
    14821 ttgatatttt ctctgccctg caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc
    14881 aacatttgca ggaaatcagc gaatccgtgc gccagatccg ccaggaaaac agcgactggt
    14941 ttagcgaaga gtaattcatt tactctcatc ccatccggga tgagaggctt ttccccccgc
    15001 cttacggctc atgcatgcat caaaaaagat gtgagcttga tcaaaaacaa aaaatatttc
    15061 actcgacagg agtatttata ttgcgcccgt tacgtgggct tcgactgtaa atcagaaagg
    15121 agaaaacacc tatgacgacc tacgatcgta accgtaacgc aatcaccact ggcagccgtg
    15181 ttatggttag cggcaccggt cacactggca agatcctgtc gattgatact gaaggtctga
    15241 ccgctgagca aatccgccgc ggaaaaaccg tagttgttga aggttgtgaa gagaaactgg
    15301 cacctctgga cctgattcgt ctcggcatga actaagtgtg tgaatgccgc cgatggcggc
    15361 attgcttttt ttacttcacg gaatatttcg ccacggtcgc tttcgcgcca tgcgctaata
    15421 aagacaagta cgcttccgtc acttttgcag taaacaagct attgtctggc aaatcatcac
    15481 caaagattgc cttaatcgcc agcaatgact ggacgcgcgc tttcccttcg gcactacttt
    15541 gtacagcctt ctgaataaca ggtaacagtg ggtcactgat ttctatcaga tttccctgtt
    15601 catcaacacc accgacataa cgcatccaac ccgcgacgcc cagcgccagc agatcgaact
    15661 tgctgtcatg cgccagatgc cagcgaacag aatccaacat ccgctgtggc aatttctggc
    15721 taccatccat cgcaatctgc caggttcgat gacgtaacgc cgggttgcta tagcgtgcaa
    15781 ttaatcggtt agcgtaatct tgcaaatcaa cgccctgcac tttcagcgtc ggcgcttgtt
    15841 cctgcaacat caagccatac gccgcataac gataatgttc atcttccatg cagtcattaa
    15901 tgtgctgata tccagcaaga taacccaaat acgccaggaa tgaatgactg ccgttgagca
    15961 tgcgcaactt catctcttca taaggcagca catcactaac cagttcggct cccgcttttt
    16021 cccattctgg acgtccggca acaaagttat cttctattac ccactggcgg aaaggttcac
    16081 aggcaacgcc cgcaggatcg cccacaccgg taagttgttc gattttcgcc agcgtatcct
    16141 ctgtcactgc gggcacaata cggtccacca ttgttgatgg gaaagtcacg ttatcttcga
    16201 tccattgtgc cagttttaca tcaacagctt tcgcgtatga agtgacaacg tcacgcataa
    16261 catgaccgtt ttctggcatg ttgtcacatg acatgacggt aaatgcggga agtcctgccg
    16321 ctttacggcg ggccagcgcc tcaacaatca ctcctgttgc tgttttcggc tggtggggat
    16381 tttgcacgtc ggcagcgacc atcgggtgat cgagcattaa ctgtccgatc gccggagagt
    16441 ggaaataccc tttttcggtg attgtcagag agacaatcgc gatttgcggt tcacacatcg
    16501 cagccaacac ggtttctaag ccatccatct gtacgtgcaa ggctttttta acgacgccaa
    16561 cgacgcgagc cgtccacgca tcggccgaca tttccgcaac ggtataaaga ttatcttgct
    16621 gttgtaaatc ggcaatttgc tgttcgccgc cgattaagtt gacctcataa tatccccagt
    16681 cactgaaatg ttctgtagca agaatatcgg catacacgcc ctgatgcgca cggtgaaatg
    16741 caccaaagcc taaatgaata attcttgggg ccaggttatt acgatcataa acagggagtg
    16801 tcgcttttgc tgataacaaa ttatttccca taacaattcc ttaaatataa atatggcaag
    16861 ctatatgttt tgttatatga ataaaaatcc cctctccggt aagagaaggg attgaggatt
    16921 tacagacttc tggaaggttg cgcagctctt acaacacgcg gttgatcttc cgcagcgtct
    16981 tccagcgcac ttaaatcacg gtctttcacc tctggcattt tcagcgcaga gattaaacca
    17041 attactgaat atgccatgat cataatggcg atcggatacc aggattccgt catggtgcag
    17101 aaaatacccg ccaggatagg accaaaaccg gaagcgataa gaccaccaat ttctttagaa
    17161 atagccatcc gggtaaagcg gtttttacag ccgaacattt ctgccatggt aatgttttcc
    17221 agagcaaata atcccagcac cgcacagtta tgaatcacaa tcagtgcaac cataatggtg
    17281 ctcggggcat agcttttatc tacaatgata gaaagcattg gccatgccag cacaatcgcg
    17341 gaggtattca taataatata cgggatccgg cgaccaattt tatcggataa ccaaccaagg
    17401 aacggaatgg tcataaagcc gagaatcgaa ctaatcatca atgcatctgt tggaattgct
    17461 ttgttaaaca ataacgtctg cactaaatag cctgcaagga aagtctgaat taacccggag
    17521 ttacccgcct gaccaaaacg cagccctgtt gccagccaga aggatttgct ctggaacatg
    17581 ctaccagcag gtgcaggttt tgctgtgggt tggttgctgt cgttaacctt ctcaaagacc
    17641 gggctttctt tcagattcat acgcaaccag atagcaaaga ccatcacgac aacgctcgcc
    17701 aggaacggta tacgccatcc ccacgcaagc agttcctctt tactgagaat gaagaacata
    17761 aaggcccaga ttgccgttgc gctcaaggtt ccgcagttag ttcccatagc tacaaatgag
    17821 gagataattc cgcgcttacc ttttggcgca tattccgcca gcatcgtacc ggcaccggaa
    17881 atttccgcac ctgcacccaa cccctgaata atacgcagcg tcaccagcaa gatgggggca
    17941 aaaacaccaa tctgtgcata ggtcggtaac acaccaatta aggtggtaca gatccccatc
    18001 atggtgatgg taataaagag cactttttta cgccctattc tgtcgcccat tttgccgaaa
    18061 ataaatgctc cgacaatacg cgccacataa cctgcaccgt aggttcccat tgccaaaatt
    18121 aacgccattg ccgttgatga ttcaggaaaa aatatttcat gaaacactaa cgctgcgccg
    18181 agcgaatata actggaaatc cataattcat agatgttttt tactattctg tgggtttttg
    18241 ggtgttttct aagttttttc agatggttgt attttttcta aaaatcccta atctcgattt
    18301 tgctgtttat ttgaggcctt tttatgtccc atatatgccc cacagatacc ccgcagccaa
    18361 aatcaacaaa atgccaaaag gttctgttcc agccctgcaa caagaaatgc tgcgacgtgt
    18421 cagtaaacgt tatgacgatg tagaagtgat catcaaatcc accagcaacg atggcctttc
    18481 agttactcgc accgccgata aagattctgc aaaaactttt gttcaggaga cgctgaaaga
    18541 tacccgggag tctgctgacg agtggtttgt tcgctaatta acgagtaaaa tcagtaacgg
    18601 ctggaaatca ttcaatactc acactatcta aaagttcacc agccaacagc ggtaagttct
    18661 ttcatacgac gtgccgcgga tcctattatt ctagtatgaa atccctaacc ggttttcaaa
    18721 ttctccagac tgctcagatt ctcttttatc ccacgctggc gctgataaat ctcgtcattg
    18781 caatcgacct gcgctatcgc tgccgcagtt cttccagttt ctgcatcgat aactccatct
    18841 tctgagcaat aaacctcacc tatgcaatat tttatgtcaa ttatttcaat tgcattaata
    18901 tcttgaatgg attacataga gttaatgtat ccagtactcc ctattctctt gcatataata
    18961 catgttgcaa cttacatctc agcgctatga aaaaacacca cctctctctt tatgaaattc
    19021 tggatttacc aaacgctaat ttatcatttc aatctacctt taagtattgc atttatctcc
    19081 ctacgaggtc atactttagg aagttaaata tgaatgataa tatacctaca gcgcgaaatc
    19141 acaaacaatc gacttgtatt acagaaaaaa acatgcctat atttttaaac ttcacagcag
    19201 gcagtatctt acctgagaat gagctagcat ctttacgtta tattgtgcag caaaatcaaa
    19261 atgatactgt aatcataaaa gaacgttata aaatggatat ccgttatatc gaatcagtca
    19321 atggttttac agtaaatcct gtatgcagta atcatttctc catatttatg gcgagacaaa
    19381 acactattgc tcgcaacctg gaacagcaga tcaacaacgg acgaagtttt gcacaaatat
    19441 ctcaggattt tatgcttcaa ttatcttcaa atataggatg gaaaaaaggg gccgaaaacg
    19501 cccttaaaaa taaaatccat tctcattcat ttgttgtaaa tcctgatgaa ttctcttgcg
    19561 acacacaatt tcttaagtgc ccaataacat tatgcgttcc agaaaaaggg gtttttgtca
    19621 agaacgcact gaactccaac atatgcactc tttatgataa gtctgcgttc atgaatctca
    19681 caagagaaca tctaccccac cctctcagca gggaaaagat agtaaaagaa atgattattg
    19741 aaaggaatat gtgttatttt gacaccataa gtcagcattt cataattatg gatgcagacc
    19801 aacagaaaca gcattgtaaa taaaatgtaa taattacata ctattagtga ttctcatgca
    19861 tcgtaagcgg ctcgccagaa ccgtattgat atttactgag cgctcagatc aactttccat
    19921 ggcaacagat cgcgtacccg gtttgccggc cagtcctgga tatgctcccg ccgacaatat
    19981 taatggccag gaaatgttta aaacaatgat tctgtttcct aaccatcaag tgtatatgct
    20041 ggctcaatta ctcaatatct atgggttgag ttctacccaa agtggctctg tagtcgcttc
    20101 ttcttacagt atgttcaggt gttgcaggca cagttgtgga gcgtagatgc gttgttggtt
    20161 taccatgctc tggtacataa aaaacgccag tgccatggta acctgatata gaaacgttaa
    20221 ttccgcgacg ttcaaactcg gcatagacgt gttctgctaa agacctcttt tgaccaaata
    20281 atgccctggc caaccagccg ttattcatat ttgcggattt ttctatttca tcaggagaga
    20341 agtctttgtt ttttattatg ttggctgagt tacaggatgt taatctgata tcatctattt
    20401 catgaagatt atgctgaaca atgcggtcga caatatctga cggtgataaa aattcatctc
    20461 cgcacttaag aagaggtaga ccagcagaac catgcccaga cagataaatt ttgttgtatt
    20521 ttcctagtgt taaatcatct ggcaggatgg ttttcatctc tgtcgctgta atacttatag
    20581 ctgcagcagc aacaacatcg ctattacttg attgtagatg gcttttattt gctccaggat
    20641 atgtaaactc cattttcctt ttatcaaaat cttgttttgt agcatcattc aggaataata
    20701 aatcatacgg ttttttcccc gttgtgactc ttgaaacatt ttggccaaga atattaagag
    20761 tatagtcatt aacggatttc attcctaaaa aaacaagtaa tccttctttc tcttgcgctt
    20821 tttcgattgt tctccctgct aaagttattg ggatggattg agattgtttt gtcggaactg
    20881 atacgctggc gctaataggt agtagggttc ttatgctaaa catacaacct ctttcttaat
    20941 tttcgaacct gtttaggatt ctgtgtaaat tcaaaataaa cctgtctgaa cattcctaac
    21001 aaacatccac cg
//
LOCUS       DAZUFB010000087        20463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZUFB010000087 DAZUFB010000000
VERSION     DAZUFB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.087"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1179
                     /gene="torY"
                     /locus_tag="ACHY3M_003522"
     CDS             79..1179
                     /gene="torY"
                     /locus_tag="ACHY3M_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFS4318360.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1204..3633
                     /gene="torZ"
                     /locus_tag="ACHY3M_003523"
     CDS             1204..3633
                     /gene="torZ"
                     /locus_tag="ACHY3M_003523"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFS4318361.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMLHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(3798..4769)
                     /gene="cmoB"
                     /locus_tag="ACHY3M_003524"
     CDS             complement(3798..4769)
                     /gene="cmoB"
                     /locus_tag="ACHY3M_003524"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFS4318362.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FETVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4766..5509)
                     /gene="cmoA"
                     /locus_tag="ACHY3M_003525"
     CDS             complement(4766..5509)
                     /gene="cmoA"
                     /locus_tag="ACHY3M_003525"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFS4318363.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5550..5945)
                     /gene="yecN"
                     /locus_tag="ACHY3M_003526"
     CDS             complement(5550..5945)
                     /gene="yecN"
                     /locus_tag="ACHY3M_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFS4318364.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(5998..6816)
                     /gene="yecE"
                     /locus_tag="ACHY3M_003527"
     CDS             complement(5998..6816)
                     /gene="yecE"
                     /locus_tag="ACHY3M_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4318365.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(6813..7379)
                     /gene="yecD"
                     /locus_tag="ACHY3M_003528"
     CDS             complement(6813..7379)
                     /gene="yecD"
                     /locus_tag="ACHY3M_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4318366.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            7689..9461
                     /gene="aspS"
                     /locus_tag="ACHY3M_003529"
     CDS             7689..9461
                     /gene="aspS"
                     /locus_tag="ACHY3M_003529"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4318367.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            9579..10031
                     /gene="nudB"
                     /locus_tag="ACHY3M_003530"
     CDS             9579..10031
                     /gene="nudB"
                     /locus_tag="ACHY3M_003530"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFS4318368.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            10060..10800
                     /gene="yebC"
                     /locus_tag="ACHY3M_003531"
     CDS             10060..10800
                     /gene="yebC"
                     /locus_tag="ACHY3M_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4318369.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            10835..11356
                     /gene="ruvC"
                     /locus_tag="ACHY3M_003532"
     CDS             10835..11356
                     /gene="ruvC"
                     /locus_tag="ACHY3M_003532"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFS4318370.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(11358..11960)
                     /gene="yebB"
                     /locus_tag="ACHY3M_003533"
     CDS             complement(11358..11960)
                     /gene="yebB"
                     /locus_tag="ACHY3M_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFS4318371.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            12031..12096
                     /gene="yobI"
                     /locus_tag="ACHY3M_003534"
     CDS             12031..12096
                     /gene="yobI"
                     /locus_tag="ACHY3M_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFS4318372.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            12235..12846
                     /gene="ruvA"
                     /locus_tag="ACHY3M_003535"
     CDS             12235..12846
                     /gene="ruvA"
                     /locus_tag="ACHY3M_003535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4318373.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            12855..13865
                     /gene="ruvB"
                     /locus_tag="ACHY3M_003536"
     CDS             12855..13865
                     /gene="ruvB"
                     /locus_tag="ACHY3M_003536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4318374.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(14012..14797)
                     /gene="znuB"
                     /locus_tag="ACHY3M_003537"
     CDS             complement(14012..14797)
                     /gene="znuB"
                     /locus_tag="ACHY3M_003537"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFS4318375.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(14794..15549)
                     /gene="znuC"
                     /locus_tag="ACHY3M_003538"
     CDS             complement(14794..15549)
                     /gene="znuC"
                     /locus_tag="ACHY3M_003538"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFS4318376.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            15628..16560
                     /gene="znuA"
                     /locus_tag="ACHY3M_003539"
     CDS             15628..16560
                     /gene="znuA"
                     /locus_tag="ACHY3M_003539"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFS4318377.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARAIAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            16576..17898
                     /gene="mepM"
                     /locus_tag="ACHY3M_003540"
     CDS             16576..17898
                     /gene="mepM"
                     /locus_tag="ACHY3M_003540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFS4318378.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            18018..18989
                     /gene="lpxM"
                     /locus_tag="ACHY3M_003541"
                     /gene_synonym="msbB"
     CDS             18018..18989
                     /gene="lpxM"
                     /locus_tag="ACHY3M_003541"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFS4318379.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(19078..>20463)
                     /locus_tag="ACHY3M_003542"
     CDS             complement(19078..>20463)
                     /locus_tag="ACHY3M_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFS4318380.1"
                     /translation="AMLTLQFHDILPGSCISRVYHETEKEYLKLEAKTEKIISDAQST
                     LLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIDPKNKIVNGLK
                     AESRSIENNYIKLLFSESGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFFAAWDFA
                     SNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFID
                     WHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQ
                     ETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFT
                     FSLFPHHGDYRVGGVVNQAYIMNHPLQIVPVKPPPGELPPSLSFFAIDTNSVIIETIK
                     LAEDGDGIIVRLYESYGLEISAMFSWVCNYHYVQYVDLQENKLDDSFYCNQYCNLEFK
                     PFEIITLRLTQ"
BASE COUNT         5417 a         4885 c         5304 g         4857 t
ORIGIN      
        1 agttatgcaa gcggttaatt cgttgtgtat ttaattatac aatgatttcg gtgttctgga
       61 atttatttag aggaatctat gcgagggaaa aaacgcattg ggttattgtt tttgctgata
      121 gcggttgtgg ttggtggcgg cgggttattg ctggcgcaaa aagccttaca taaaacgtcg
      181 gatacagcat tttgcctttc ctgccactcg atgagtaaac cttttgagga atatcaggga
      241 actgtccact tttcaaacca gaaagggata cgtgcggaat gtgcagattg ccatattcca
      301 aagtcaggga tggattattt atttgctaaa ttaaaagcat ctaaagatat ttatcatgaa
      361 tttgttagcg gcaaaataga cagtgacgat aagttcgaaa ctcatcgcca ggaaatggcc
      421 gaaacagtat ggaaagaatt aaaagcaact gactctgcaa cgtgccgtag ttgccattct
      481 tttgatgcca tggatattgc ctcgcaaagt gaatctgcgc agaaaatgca taacaaagca
      541 caaaagggcg gcgaaacctg tatcgattgt cataaaggca ttgcccattt tccgccagaa
      601 ataaaaatgg atgacaacgc ggcgcatgag ctggaaagtc aggccgctac ttcagtgact
      661 aatggcgcac atatttatcc tttcaaaact tctcgcatag gcgagctggc taccgtgaat
      721 cctggtaccg atctcaccgt cgttgatgcc agtggcaaac agccgatcgt tctgttgcag
      781 ggttatcaaa tgcagggcag tgaaaacacg ctctacctgg cggcaggtca acggctggcg
      841 ctagccacat taagtgaaga aggtatcaag gcgctcacgg taaacgggga atggcaggct
      901 gacgaatacg gcaatcaatg gcgtcaggcg tctttacagg gtgcgcttac cgatcccgca
      961 ttagcggacc gtaaaccgct atggcaatac gctgaaaaac ttgacgatac ctattgcgct
     1021 ggttgccatg cgcctattgc cgctgaccat tacaccgtca atgcatggcc gtccattgcc
     1081 aaaggaatgg gtgcgcgaac cagcatgagc gaaaacgaac tggacatttt aacgcggtat
     1141 ttccagtaca acgccaaaga tattaccgag aaacagtgat cctcgttgat cagggaggaa
     1201 gttatgacat taacaagacg tgaatttatt aaacacagcg gtattgccgc tggagcgctg
     1261 gtggtgacat cggcagcacc gctccccgca tgggcagaag agaagggcgg taaaatcctc
     1321 accgcgggac gttggggagc gatgaacgta gaagtgaagg acggcaagat tgtttcttca
     1381 acaggcgcgc tggcgaaaac cataccgaac tccttacagt ctacggcggc ggatcaggta
     1441 cacaccacgg cacgtattca gcatccgatg gtaagaaaaa gctatctcga taatccactg
     1501 caaccggcga aaggtcgtgg cgaagatact tatgtacagg tgagctggga gcaagcatta
     1561 aaactgattc atgagcaaca cgatcgcatt cgcaaagcta acggaccatc tgctattttt
     1621 gccggatcat acggctggcg ctcttctggc gtgttgcata aagcgcaaac cttactgcaa
     1681 cgttatatga acctggcggg cggttattcc gggcatagcg gcgattattc aaccggggca
     1741 gcacaggtga tcatgctgca tgtggtcggt tctgtagagg tgtatgaaca gcagacctcc
     1801 tggccgctga ttctggaaaa cagccaggct gtggtgctgt gggggatgaa cccgctgaat
     1861 accttaaaaa ttgcctggag cagtaccgat gagcaagggc tggaatactt ccatcagctg
     1921 aaaaaatctg gcaaaccagt gattgccatt gatcctatcc gctccgaaac catcgagttc
     1981 tttggtgata atgccacctg gatcgcgccg aatatgggca ccgacgtggc actgatgtta
     2041 gggattgcgc ataccctgat gacacaaggt aaacacgata aagtgttcct tgaaaaatac
     2101 accaccgggt atccgcagtt tgaagagtat ctgacaggta agagcgataa tactccgaag
     2161 agcgcggcct gggcggcgga aattactggc gtaccggaag cgcagatagt caaactcgct
     2221 gaactgatgg cggcgaatcg cacaatgctg atggctggct ggggaattca gcgccagcaa
     2281 tatggtgaac agaaacactg gatgctggtg acgctggcgg cgatgttagg gcagattggt
     2341 acaccgggcg gcggttttgg tttctcttat cattactcca acggtggcaa tccgacgcgt
     2401 gttggcggcg tgctgccgga aatgtctgcg gcgattgccg gacaggccag cgaggccgct
     2461 gatgatggcg gaatgacggc gattcctgtt gcccgtatcg tcgatgcgct ggaaaatcct
     2521 ggtggcaaat atcagcataa cggtaaagaa caaacgtatc cgaatatcaa aatgatctgg
     2581 tgggcgggtg gcggtaactt tacccatcac caggatacta atcgtctgat caaagcatgg
     2641 cagaaaccgg agatgatcgt cgtttctgaa tgctactgga ccgcagctgc taaacatgca
     2701 gatatcgtat taccgatcac cacatcgttt gagcgcaatg acttgacgat gaccggtgat
     2761 tacagcaacc agcatattgt gccgatgaag caggctgttg ctccgcaatt tgaagcgcgt
     2821 aacgattttg acgtatttgc cgatcttgct gaattactga aacctggcgg aaaagagatc
     2881 tataccgaag gtaaagatga aatggcgtgg ctgaaatttt tctatgatgc cgctcagaaa
     2941 ggtgctcgtg cgcaacgcgt gactatgccg atgtttaatg ccttctggca gcaaaataaa
     3001 ctgatcgaaa tgcgccgcag cgagaagaac gaacagtacg ttcgttatgc tgatttccgc
     3061 gccgatccgg tgaaaaatgc gctgggtacg ccaagcggca aaattgagat ttactccaaa
     3121 acgctggaaa aatttggcta taaggattgc ccggcacatc caacctggct tgcgcctgat
     3181 gagtggaagg gtaccgccga cgagaagcag ttgcagcttc tgaccgcaca tccggcacac
     3241 cgtttacata gtcagcttaa ctatgcggaa ctgcgtaaaa aatatgcggt tgccgatcgt
     3301 gaaccgatca ctattcacac cgaagatgct gctcgatttg gtattgcgaa tggcgatctg
     3361 gtgcgtgtct ggaacaaacg tggtcagatt ttgacaggcg cggtggtgac tgacgggatc
     3421 aaaaaaggcg tggtatgcgt gcatgaaggt gcatggccag atctggaaaa cggcttgtgt
     3481 aaaaacggca gtgcgaacgt gttaacggcg gatatcccca gctcgcagct ggcaaatgcc
     3541 tgtgccggta actctgcgct ggtgtatatc gaaaaatata cgggtaacgc accgaagtta
     3601 acggcatttg ataagcctgc tgttcaggcg taagaagtga atagcgcccg gaaacgggcg
     3661 ctgaggttat gcaaacgtaa aactgcctga tgcgctatgc ttatcaggcc tacatagtcc
     3721 ctgcaataga ttgaattagc aagggtttgt aggccggata aggcattcaa gccgcatccg
     3781 gcagtaatgc cagaccttta cggcttgcgc gcaatcagca ccgcgcgttt aggcgcagga
     3841 taaccttcaa ctgtcttact cggatcatgt gggtcgagga aatcggacag agattcggta
     3901 accatccatt cagtgcgtcg ctgctcttca gtagtggtaa cgcacacatc cacaacgcgg
     3961 atatctacaa aaccacactt cttcagccag tttttcagcg ccagcgcgga agggatgaaa
     4021 tagacattac gcatttgcgc gtaacgatcg cctggtacca gcaccgtgtt ctcatcgcca
     4081 tcaataacca gcgtttccag caccagttca ccctcattca ccagttgatc tttcaactgc
     4141 cagagatgct ccagcggcga acggcgatgg taaagcacgc ccatcgaaaa gacggtatca
     4201 aaggctttca gtgccggaag ctgttcaata cctaacggta acaaatgtgc gcgctgatcg
     4261 ttacccagta gtttacgcac cgtttcaaac tggcacagaa acagttgcgt agggtcgatc
     4321 cccaccgcga ggtgcgcgcc tgcgccaatc atgcgccaca tgtgataacc gctgccgcag
     4381 ccgacatcca gaatggtgcg tccggttaaa tcagaaagat ggggcagaac acgatcccat
     4441 ttccagtcgg aacgccattc ggtatcgatg ttgacgccat acagtgagaa cggccctttg
     4501 cgccatggca tcaggttccg cataagcgtt tcaatgcgtt taatttgccc ggtgctcagt
     4561 ggctcttcgc tttcggcggt tacgctatgc aataaatcca gacgatacgg tttaatttca
     4621 ggcagaaatt ccaccgcgtt ggaccactgc ttaaacagcc cgtgctgctg ctcgcgctgc
     4681 cagttagcaa tctgcgcggg cagcgtttcg agccagtgtg aaagatgatt tttggcaatc
     4741 agagaataaa agttaccaaa gtcgatcatg cagcgtcctc tgcttttaat gccaccagtg
     4801 aaccaaagtt aaagcactgg aaccacagct cgctatgctc aaaaccggct ttatgcaggc
     4861 gtgctttatg ggtttccacg gaatcggtca gcatcacgtt ttccagcatg ctgcgcttct
     4921 ggctgatctc cagttcgctg taaccgttgg cacgtttaaa gtcgtggtgc atgttgaaca
     4981 acagttcacc aactttggca tcttcgaaac tgaatttttc cgaaagcacc agcgcgccgc
     5041 ccgggttcag tccttgataa attttatcca gtaatgcctg gcgctcggaa ggttccagga
     5101 attgcagggt aaaattcagc accaccatcg atgcgttttc aatggcgata tcgcgaatat
     5161 caccttcaat gacgtctact ggcgtagggg ctttataggc gtcaatatga cgacggcagc
     5221 gttcaatcat cgccggggag ttgtcgatgg caataatttt gcaattatca tgatgaatgt
     5281 tgcgacgcac cgagagcgtc gccgcgccca gagaacaacc cagatcgtaa acctgcgtac
     5341 caggttgaac gaagcgctcg gctaacatac caatcatgga aataatattg gaatagccgg
     5401 gaacggaacg ctggatcata tccgggaaga cttcagctac ccgttcatca aaggtccagt
     5461 cgcccagtct ggcgataggg gcagaaaata gcgtgtcgcg gtgagacata acgtaaaaat
     5521 ccgggaaaaa gaaagtggcg tattgtgcgc taacgcaggg agaaaaccaa ctcccagggc
     5581 atataccaaa gattcgccag caccatcagc aacagcgcac accaggtggc gctcatgccg
     5641 gaacgtcgcc agcgaaacag acggtgatga aaaccgtaat aatgcatcag acgaccagca
     5701 agcaaaacga tgccgcaaat atgcaccatc caggtttctg cgccattcat ttccataaac
     5761 agcatcagca caatcgcgat gggaatatat tccaccgcgt taccatgaat gcgaatagcg
     5821 ctttgcagtt cgctaaaacc accgtcacca taggcaacgc ggtactgcaa acgcaagcga
     5881 acgacatcaa aagagaactt cattaataac aacgcactta aaacggcata tagcgcgctt
     5941 accatacaaa ctccctttaa aatggccgat ggcacctgtc tatgataggt ggcaaattca
     6001 gaaaagagaa gattgctgtg gaatagccgg aactgctccg atctctggaa gcgttttacg
     6061 taagtcttcc cacagggtat gtaccagttc cggggcctgg gcaatgtctg gcgtatgtaa
     6121 aaaaagataa ggcgtagtgg tctgatgcca ctgcgctaat ttctgtaacc agacctgaaa
     6181 taattcccgg ttttgcgtca tatcatcact accgataaaa cggatcagcg gatttgtcgc
     6241 cgtcagtaca gcatgtaccg gaactttagg tttttttcgt tgagcgtcgc gaatagcttc
     6301 actgtgtgga catgctgcat gaaccgggcg gctgtctaaa atcacccgat taacgccgcg
     6361 ctgatgtaaa ccgcgattaa gcgtttgttc ctcttcccct ttggcgaaaa actgtggatg
     6421 gcggacttcc acaccataat taaattcgcc agggagagaa tcgagaaaat gccaaagcgc
     6481 aggcagctcc cgtgggccga atgtggcagg cagttgcagc cagtattgcc caatgcgcgg
     6541 agccaacggt gacatgcggg tcaaaaattc agtcactaaa tcatcgcaat gtcgtaatgc
     6601 tgcctgatgc gaaatggtcg ccggaaactt aaaacagaag cggaagtcat ctgtggtctg
     6661 ttcacgccag cgcaggacaa cctcgggttt cggcagggcg taaagcgtgg tgttgccctc
     6721 cacgcagtta aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt
     6781 aggatgcgac cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg
     6841 ctacgcacac gggcgatgcg tgggtagata tggttgatgc tgttattgtg ctgctcggcg
     6901 ctagcggcac tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg
     6961 cgggcggtgg attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata
     7021 cctcggcggc gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca ctgacgtttg
     7081 atgatttcga tatcactgtc agttgcacct aatgcagctg gatgttgcca ccagttttcg
     7141 ggcaacactt tagcggggga gggggcatca accggctgtt ttaatgcttc ggcgtaatcg
     7201 gcagaccagc caacgcgcac cagaaacacg ggctgaccgc tggcgcgaaa tttcgccgcc
     7261 agcttcccgg cgcgattaac tacctcatcg gcagtatgtg gaccaccggc aaaaggtaag
     7321 atgccttctt gtaaatcgat caccaccagc gcagtggttt tagcattaag ttcaagcata
     7381 atgactcctg aaaaaacaaa atcgtgcctc acaccttaat gcgattagcc gatggcggaa
     7441 cgacaaattt tgttaatttt tatgagttcg ggcaacacct ttatagcaaa ctggcgtaga
     7501 gtctgatttc aggcccgata taaagtggta acgaggcgtt ccagcgcggg ccaggctctt
     7561 acggtgcggc agaatttcca gtataatagc cgcctttttt cattcagttg tgacatacag
     7621 ctaacgctgc gacttggtca cctgcgcagc agggggcatc cgccggcagc taagttaagg
     7681 gatatctcat gcgtacagaa tattgtggac agctccgttt gtcccacgtg gggcagcagg
     7741 tgactctgtg tggttgggtc aaccgtcgtc gtgatcttgg tagcctgatc ttcatcgata
     7801 tgcgcgaccg cgaaggtatc gtgcaggtat ttttcgatcc ggatcgtgcg gacgcgttaa
     7861 agctggcctc tgaactgcgt aatgagttct gcattcaggt cacgggcacc gtacgtgcgc
     7921 gtgacgaaaa aaatattaac cgcgatatgg cgaccggcga aatcgaagtg ctggcgtcct
     7981 cgctgactat catcaaccgc gcagatgttc tgccgcttga ctctaaccac gtcaacaccg
     8041 aagaagcgcg tctgaaatac cgctacctcg acctgcgtcg tccggaaatg gctcagcgcc
     8101 tgaaaacccg cgctaaaatc accagcctgg tgcgccgttt tatggatgac cacggcttcc
     8161 tcgacatcga aactccgatg ctgaccaaag ccacgccgga aggcgcgcgt gactacctgg
     8221 tgccttctcg tgtgcacaaa ggtaaattct acgcactgcc gcaatcccca cagttgttca
     8281 aacagctgct gatgatgtcc ggttttgacc gttactatca gatcgttaaa tgcttccgtg
     8341 acgaagacct gcgcgctgat cgtcagcctg aatttactca gatcgatgtg gaaacttctt
     8401 tcatgaccgc gccgcaagtg cgtgaagtga tggaagcgct ggtgcgtcat ttgtggctgg
     8461 aagtgaaggg cgtggatctg ggcgatttcc cggtaatgac ctttgcggaa gcagaacgcc
     8521 gttatggttc tgataaaccg gatctgcgta acccgatgga actgaccgac gttgccgatc
     8581 tgctgaaatc tgttgagttt gcagtatttg cggggccggc gaacgatccg aaaggccgtg
     8641 ttgccgctct acgtgttccg ggtggcgcat cgctgacccg taagcagatc gacgaatacg
     8701 gtaacttcgt taaaatctac ggcgcgaaag gtctggctta catcaaagtt aacgaacgcg
     8761 cgaaaggtct ggaaggtatc aacagcccgg tagcgaagtt ccttaatgca gaaatcatcg
     8821 aagccatcct ggatcgtact gccgcgcaag atggcgatat gattttcttc ggtgccgaca
     8881 acaagaaaat tgttgccgac gcgatgggcg cactgcgcct gaaagtgggt aaagaccttg
     8941 gtctgaccga cgaaagcaaa tgggcaccgc tgtgggttat cgacttcccg atgtttgaag
     9001 acgacggtga aggcggcctg acggcaatgc accatccgtt cacctcaccg aaagacatga
     9061 ctgctgcaga actgaaagct gcaccggaaa atgcggtggc gaacgcttac gatatggtca
     9121 tcaatggtta cgaagtgggc ggtggttcag tacgtatcca taatggtgat atgcagcaga
     9181 cggtgtttgg tattctgggt atcaacgaag aggaacagcg cgagaaattc ggcttcctgc
     9241 tcgacgccct gaaatacggt actccgccgc acgcaggtct ggcattcggt cttgaccgtc
     9301 tgaccatgct gctgactggc accgacaata tccgtgacgt tatcgccttc ccgaaaacca
     9361 cggcggcagc gtgtctgatg actgaagcac cgagctttgc taacccgact gcactggctg
     9421 agctgagcat tcaggttgtg aagaaggctg agaataactg atatgactca aatacacgaa
     9481 atcattcgcg ttgcatcaag gcggcaactg agtgaactcc catgagcata gataactatg
     9541 tgaatgggat gagcgaaggc agtcaacgaa gaggcagcgt gaaggataaa gtgtataagc
     9601 gtcccgtttc gatcttagtg gtcatctatg cacaagatac gaaacgggtg ctgatgttgc
     9661 agcggcgtga cgatcccgat ttctggcagt cggtaaccgg cagcgtggaa gagggtgaaa
     9721 ccgcgccgca agctgccatg cgcgaagtaa aggaagaggt caccattgat gttgtcgctg
     9781 aacaactgac cttaattgac tgtcagcgca cggtagagtt tgaaattttt tcacatttac
     9841 gtcatcgcta tgcgccgggc gtgacgcgta atacggaatc atggttctgt cttgcgcttc
     9901 cgcacgagcg gcagatcgtt ttcactgaac atctggctta caagtggctt gatgcgcctg
     9961 ctgcggcggc gctcactaag tcctggagca accggcaggc gattgaacag tttgtcatta
    10021 acgctgcctg aacaggcagg gctatctgga gatattttta tggcaggtca tagtaaatgg
    10081 gccaacacca gacatcgtaa agctgcgcag gatgcaaagc gcggtaaaat cttcactaaa
    10141 atcattcgtg agctggtaac cgcggctaag ctgggcggtg gcgatccgga cgctaacccg
    10201 cgtctgcgtg cagcaattga taaagcactg tctaacaaca tgacccgcga cacactgaac
    10261 cgcgcaattg cacgtggtgt gggcggtgat gatgatgcaa acatggaaac catcatctac
    10321 gaaggttacg gtcctggcgg cacggcaatc atgattgaat gtctgtctga caaccgcaac
    10381 cgtaccgttg ctgaagtgcg tcatgcattc agcaaatgtg gcggtaacct cggtactgat
    10441 ggttccgtag cctatctgtt cagcaaaaaa ggcgtgatct ccttcgagaa aggcgatgaa
    10501 gacaccatca tggaagcagc tttggaagca ggtgctgaag acgttgtgac ctatgatgac
    10561 ggcgcgattg atgtctacac cgcatgggaa gaaatgggta aagtgcgcga cgctctggaa
    10621 gcggcaggtc tgaaagcaga cagcgcggaa gtttccatga tcccgtccac caaagctgat
    10681 atggatgcag aaaccgcacc gaaactgatg cgtctgatcg atatgctgga agattgcgac
    10741 gacgtgcagg aagtttacca taacggtgaa atctctgatg aggttgcagc gactctctga
    10801 tgaggcctgc taaacagcaa aacggagacg cgtgatggct attattctcg gcattgatcc
    10861 gggttcgcgc gtgaccggct atggcgtcat ccgccaggta ggcaggcaac tgtcctacct
    10921 gggtagtgga tgcatacgca ccaaagtgga tgatttaccg tctcgtctga agctcatcta
    10981 tgcgggcgtg acggaaatca tcacccagtt ccagcctgat tatttcgcca ttgaacaagt
    11041 ctttatggcg aagaacgctg actcagccct gaaactgggc caggcgcgcg gcgtggcgat
    11101 tgtggcggcg gtgaatcagg aattgccggt atttgaatac gcggcacgtc aggtaaagca
    11161 aacggtggta ggtattggta gcgccgagaa aagccaggtg cagcatatgg tccgcacctt
    11221 gctgaaactg cccgctaatc cacaagcgga tgccgctgat gcgttagcta tcgcgattac
    11281 ccattgtcac gtcagccaga atgcaatgca gatgagcgaa tcgcggctga acctggcgag
    11341 agggcgactg cgttaagtta taccgcctcg gtcagttcag gctgaggcgt ttcaactccc
    11401 tccgcgtgaa tcaacaccaa acccggatga tgccacaaac tggctggcgt gacggtttta
    11461 cgctcccacg gaatagaacc taagaaccag aatttccaga aagtgagttt tgcattcgga
    11521 ttgctattta acagttgtcc aaacgtctct atttcaccca ccggaataca tagcgcctct
    11581 ttataaatat caaagacaaa ttttgaacag aactggcggg aagattcgta tttaaaaccc
    11641 gtgtggtaaa gtttgcgtag ccgggaggga acctgttcaa caattcgttg tttctgttgt
    11701 tctgtcagcc cggcgtctaa tcgctttata gcatagcgtt gattagcaga gcgtttaata
    11761 aaacgggata gcgtagtgat ggttgagagg ggaacacggc tttctgcaac cagaaagtct
    11821 tcaccgttat gaccgataat gatcccgacg tgattactcc agcagtttga tgcagctgat
    11881 atttgaccaa ataaggcagc acctatacat gtaaagacga tatcaccaat ttcatattca
    11941 gcagggtaat taatattcac gttgtcattc cattgaaata gatatgaata tacgtaatag
    12001 cataaaaaat ttattatttc aggagtcgca atgtatattt ttattacgca tttcttcact
    12061 gaatatgtaa tattcaaata tttgcttcca atataacctg tagaataaat tatactgcgc
    12121 catttttcag atcgttggga cgcctcgcaa gttttcttca tccttcgctg gatatctatc
    12181 cagcattttt ttatcataca gcattatctt tgattcatta cgcaggagcg tcatgtgata
    12241 ggcagactca gaggcatcat cattgaaaaa caacccccgc tggtgttaat tgaagtgggc
    12301 ggcgtaggct atgaagtgca tatgccgatg acctgttttt atgaactccc tgaagcgggt
    12361 caggaagcga tcgttttcac ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt
    12421 tttaacaata aacaagagcg cacattgttc aaagagttga tcaaaaccaa cggcgtcggc
    12481 ccgaagttgg cgctggcgat cctctccgga atgtcagcgc agcagttcgt taatgccgtt
    12541 gagcgtgaag aagtgggggc actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa
    12601 cgcttgattg ttgaaatgaa agaccgattt aaaggtttgc atggcgatct ctttacgcca
    12661 gccgccgacc tggtactcac gtcacctgcc agcccgacga ccgacgatgc tgaacaggaa
    12721 gcggttgccg cgctggtggc gctgggctat aaaccacaag aagcaagccg catggtgagc
    12781 aaaatcgctc gccctgacgc cagcagtgaa acattaattc gcgaagccct acgcgccgcg
    12841 ttatgaggta aaggatgatt gaagcagacc gtctgatttc tgccggtacc actttgccgg
    12901 aagatgtggc agatcgcgcc attcgcccta aattactgga agagtatgtt ggtcagccgc
    12961 aggttcgttc acagatggag attttcatca aagcagcgaa actgcgcggc gatgccctcg
    13021 atcatctgtt gattttcggt cctccggggt tgggtaaaac tacgcttgcc aacattgtcg
    13081 ccaatgaaat gggcgttaat ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg
    13141 atctggcagc gatgctcact aaccttgaac cgcatgacgt gttgtttatt gatgagatcc
    13201 accgtctttc gccagtggtg gaagaggtgt tgtatccggc aatggaagac taccaactgg
    13261 atatcatgat tggtgaaggt ccggcggcac gctccattaa aattgatttg ccgccgttta
    13321 ccctgattgg tgcaaccacg cgcgcaggtt cgctgacatc accgttgcgc gatcgttttg
    13381 gtattgtgca gcgtctggag ttttatcagg tgccggatct gcaatatatc gtcagtcgca
    13441 gcgcacgctt tatggggctt gagatgagtg atgacggcgc gctggaagtt gctcgtcgag
    13501 ctcgcggtac gccgcgcatt gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag
    13561 tgaagcacga tggcaccatc tcggcagata tcgctgctca ggcgttggat atgttgaatg
    13621 tcgatgctga aggtttcgat tatatggacc gcaagttatt gctggcggta atcgataagt
    13681 tctttggtgg gccggtaggt ctggataacc ttgcggcagc cattggcgaa gaacgtgaaa
    13741 ccattgagga tgtgctggaa ccttatttga ttcagcaagg ctttttgcag cgtacaccgc
    13801 gtgggcgtat ggcgacgacg cgggcgtgga atcactttgg cataacgccg ccagaaatgc
    13861 cgtaagtcgg attggtcgct cgcgccgcat ccgacactgg cagtgtgtct gatgcgacac
    13921 tgttcgcgtc ttatcaggcc agccagagat agcgaaccgt aggtcggata aggcgctcgc
    13981 gccgcatccg acaaatgtgt tcagcgatag attagctggc ctgctttttc atcatactga
    14041 gaataaataa cagtgccgca catagcacca ccgacggacc cgccggcgta tcgtaaaacg
    14101 cggaaaaggt taaaccgcca gttactgcca ccatccccac caaaacagcg acaccagcca
    14161 tctgttccgg cgtacgggca aagcgacgcg cagtagctgc aggaataatc agcagtgaag
    14221 taataatcaa cgcgccaaca aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca
    14281 tcaacaacaa tttcacgcgc tgtaatttca caccatcaac aaacgccaga tccgggctaa
    14341 tcgtcatcga cagcaaattg cgccattgcc agaacaaaat agccaccacg atgaccacgc
    14401 caatcgcaat agcgatgaga tcttctggcg tcactgccag caaatcaccg aacaggtaag
    14461 ccatcaaatc aacacgaatg ttagacatca gactaacgac caccaggccc agcgacaggg
    14521 cactgtgcgc cataatccct aataacgtgt caatcgccag ctgtggacgc ttctccagcc
    14581 ataccagacc gcccgccagg agcagcgtaa cggcaattac cgcatagaat ggattcacgt
    14641 ccagcaacaa accaaacgcg acgccaagta atgaggcatg agccagcgta tcaccgaaat
    14701 aagacatacg acgccagact acaaacgaac ccagcggacc cgcggcacag gcgagcatga
    14761 tcccggctaa ccaaccggga aataataatt caatcatgag cgatcatttc cccgacgcaa
    14821 aacaattcgt ccctgtaaat cgtgacgatg attatgatga tggcgataga tacccagttg
    14881 ttcagcacca cgaggaccaa acatagaaat aaactccgga tgtagggaaa caacttccgg
    14941 tgtgccggaa caacaaatgt ggtgattcag acacagcact tcatcggttt ttgccattac
    15001 cagatgcaga tcgtgagaga ccattaaaac gccacaatcc agttcgcgac gcaactggtc
    15061 aataaggtca tataacgcca cctggccatt tacatccacg ccttgagtgg gttcatccag
    15121 cactaataat tgcggtcgat ttaacaatgc tcgcgctaac agtacacgct gtgtttcgcc
    15181 accagagagc ttttgcatcg gtgcgttaat cagatgcccg gcctggacac gtttcagtgc
    15241 aggcaaaata tcttctttat gtgtgccagg gcgtaagcgt aaaaaacggt ttacggtcag
    15301 tggcaacgtg gtgtcgagat acagcttctg cggtacatag ccgatgcgta gttttccgtt
    15361 gcgcttgata accccttcat cgggtgttac cagcccgagc actacccgta ccagtgtcga
    15421 cttacctgcg ccatttggcc caagtaaagt caaaattttt ccaggtttaa gttccagcga
    15481 cacatcagag aggacgcggc gttggccaaa agaaaccgag acattttcca gggaaaccag
    15541 acttgtcatg ttaattttag tcttgcagta gtcatgaaat gttataatat cacacttctc
    15601 atattcatta cgatgattgg tcgcattatg ttacataaaa aaacgcttct tttcgcagca
    15661 ttatccgccg ctctctgggg aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg
    15721 cttaaacccg ttgggttcat cgcttctgcc attgctgatg gtgtaacaga aacggaggtt
    15781 ttactgcctg acggcgcttc agaacatgat tattcactgc gtccatcgga tgtaaaacgc
    15841 ttacagaacg cggacttagt cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa
    15901 ccggtaagca aattaccagg agcgaagcag gtaacgattg cgcagcttga agatgtgaaa
    15961 ccgctactga tgaaaagtat tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt
    16021 gacgaagatc accatcacgg cgatttcaac atgcatcttt ggctttcccc agagatagcg
    16081 cgggctatag cggttgcaat ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc
    16141 aaacttgacg ccaacctgaa ggattttgag gcacaattag cctcaaccga aacgcaggtt
    16201 ggtaacgagc tcgcgccgct caaggggaaa ggttatttcg tttttcacga tgcttacggc
    16261 tatttcgaaa aacagtttgg actgacaccg cttggtcatt ttaccgttaa ccctgagatt
    16321 caacctggcg cgcagcgttt acatgaaata agaacacagt tggttgagca aaaagcaacc
    16381 tgcgtttttg ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc acgagggaca
    16441 tccgttcgta tgggaacgtt ggatcccctg gggacgaata tcaaactggg taaaacaagc
    16501 tattcagaat tcctgagtca attagccaac cagtatgcga gctgcctgaa aggagattaa
    16561 tgaggaagtg attacgtgca acagatagcc cgctctgtcg ccctggcgtt taataattta
    16621 ccgcgaccac accgcgttat gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc
    16681 tggcggcctt atgtttatca ccgtgatgcc acgccaattg tcaaaaccat tgagctggaa
    16741 cagaacgaaa ttcgttcgct cttacctgaa gccagtgagc cgattgatca agctgcacaa
    16801 gaagatgaag ccattcccca ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg
    16861 catgaatatg ttgtttccac tggcgatacg ctaagcagca ttctcaatca gtatggtatt
    16921 gatatgggtg atatcaccca actggctgcg gccgacaaag aattgcgtaa cctgaaaatc
    16981 ggtcaacaac tctcctggac attaaccgcg gacggcgaac tgcagcgcct cacctgggaa
    17041 gtgtctcgtc gtgaaacccg aacctatgac cgtactgccg ctaacggttt taaaatgacc
    17101 agcgaaatgc agcaaggaga gtgggttaac aatctgctga aaggtaccgt cggggggagc
    17161 tttgttgcca gcgccagaaa cgccggttta accagcgccg aagtgagcgc agtgattaaa
    17221 gccatgcagt ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta
    17281 atgtctcgag aaatgcttga tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg
    17341 cgttcagaag gtaaagatta ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt
    17401 aacggtactg gtctggcgaa aggattcttg cgattcccga cggcgaaaca gttccgtatc
    17461 tcgtctaact ttaacccgcg tcgaactaat ccggtgacag gtcgcgttgc accacacaga
    17521 ggtgttgatt tcgccatgcc acaaggtacg ccagtgcttt cagtgggtga cggtgaagtg
    17581 gtggttgcca aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc
    17641 tacaccacgc gttatatgca cttgcgcaag attctggtga aaccgggaca gaaggtgaaa
    17701 cgtggcgacc gtatcgcgct ttccggtaat accggacgtt caactgggcc gcatctgcac
    17761 tatgaagtat ggataaacca gcaggccgta aacccgctga cggcaaaact gccgcgtact
    17821 gaagggctga ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg
    17881 cagctacggt ttgattaatt aacatccatt cgcagccggt acgcagtcag taccggcttt
    17941 ttttatttgg tgcggggcaa gttgcgccgc tacactatca ccagattgat ttttgcctta
    18001 tccgaaactg gaaaagcatg gaaacgaaaa aaaataatag cgaatacatt cctgagtttg
    18061 ataaatcctt tcgccacccg cgctactggg gagcatggct gggtgtagca gcgatggcgg
    18121 gtatcgcttt aacgccgcca aagttccgtg atcccattct ggcacggctg ggacgttttg
    18181 ccggacgact gggaaaaagc tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc
    18241 cagaacgtag tgaagctgaa cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc
    18301 aagcgatggc aatgatggct gagttggcaa tacgcggacc ggagaaaatt cagccgcgcg
    18361 ttgactggca agggctggag atcatcgaag agatgcggcg taataacgag aaagttatct
    18421 ttctggtgcc gcacggttgg gccgtcgata ttcctgccat gctgatggcc tcgcaagggc
    18481 agaaaatggc agcgatgttc cataatcagg gcaacccggt ttttgattat gtctggaaca
    18541 cggtgcgtcg tcgctttggc ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca
    18601 tccagtcggt acgtcagggg tactggggat attatttacc cgatcaggat catggcccag
    18661 agcacagcga atttgtggat ttctttgcca cctataaagc gacgttgccc gcgattggtc
    18721 gtttgatgaa agtgtgccgt gcgcgcgttg taccgctgtt tccgatttat gatggcaaga
    18781 cgcatcgtct gacgattcag gtgcgcccac cgatggatga tctgttagag gcggatgatc
    18841 atacgattgc gcggcggatg aatgaagaag tcgagatttt tgttggtccg cgaccagaac
    18901 aatacacctg gatactaaaa ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata
    18961 agcgcaaaga tctttatccc atcaaataaa aaacagcctc tcaaattatt gagaggctgt
    19021 acttgataaa aaatctcaat gagtgttctc accattttga gtttttagat tagtacgcta
    19081 ttgagttaat cgtagcgtga taatttcaaa aggtttgaat tcaagattgc aatattgatt
    19141 gcaataaaaa ctgtcatcta atttattttc ttggagatcg acatattgaa cgtagtgata
    19201 attacatacc cacgaaaaca tggctgaaat ttctaatcca taactctcat ataatcttac
    19261 gattataccg tcgccgtctt cagctaattt tattgtttcg atgataacgc tgttagtatc
    19321 gatagcaaaa aatgatagtg aagggggtag ttcacccggg ggtggtttga cagggacgat
    19381 ctgtaaggga tgattcatta tataggcctg gttaacgaca ccacccacac ggtaatctcc
    19441 atgatgagga aataaggaga atgtgaattt ttgtttacca atatctgcaa aattattaat
    19501 caggaaatta tcataattat ctgtcttaac aacttcccca ggtttgtgct gcgaacgcaa
    19561 caaagttaac tctagcgtct ggtctttgac acgatagcca tatttgtttt ttgctattaa
    19621 agcaatgcca gtttcttgat cagaaaggtc gacccagtga tgtgctggta tttcatcttt
    19681 ggcaaaagcg aacgaatcat cgctatgcgt tggtctgtca attacaccga actgaatttg
    19741 acattgtgca agagatgttt gtactgatac cgggaatttt actttgagag aaacattagg
    19801 ttcgtgccaa tcaataaaag tttggacatc aacgcgtgga ctgtcctgtg tcagggtaaa
    19861 tgcgaagcga agataagagg aattgtagtg ataaatcagc gtcatcgtag ttttaggccc
    19921 gctgatcaca gttgtcattt tttcagcgag taaaacatat gattccccat cacgataatt
    19981 tgaagcaaaa tcccaggccg cgaaaaaacc tgcatcttcg tggtatgcac ggatctcact
    20041 gtgcatattt tcagtaatgt attctttacc atatctttta tcatatagcg aaattaaatc
    20101 accgctttct gaaaaaagaa gtttaatgta attattttca atacttcgag attctgcctt
    20161 cagaccattt acgatcttat tctttggatc aatcacagca taaccataag agtttactcg
    20221 tgctttgagc cagttgttgt ttatattgat ccattcgtta cgagcaaaac aggtggtatt
    20281 aaataaaatg tgcggatctt tataagatga agtgtcaatt ttacttaata aagtagactg
    20341 cgcatcagaa ataattttct cggtttttgc ttctagttta agatattcct tttcagtttc
    20401 atgataaact cttgaaatac atgagccggg aaggatgtca tggaattgca aggtcagcat
    20461 cgc
//
LOCUS       DAZUFB010000088        20036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZUFB010000088 DAZUFB010000000
VERSION     DAZUFB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.088"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..423)
                     /locus_tag="ACHY3M_003543"
     CDS             complement(<1..423)
                     /locus_tag="ACHY3M_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFS4318381.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPGSRVKIGSN"
     gene            complement(580..1068)
                     /locus_tag="ACHY3M_003544"
     CDS             complement(580..1068)
                     /locus_tag="ACHY3M_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318382.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(1081..3888)
                     /locus_tag="ACHY3M_003545"
     CDS             complement(1081..3888)
                     /locus_tag="ACHY3M_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4318383.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVAVSDA
                     NGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3875..4030)
                     /locus_tag="ACHY3M_003546"
     CDS             complement(3875..4030)
                     /locus_tag="ACHY3M_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFS4318384.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(4039..4413)
                     /locus_tag="ACHY3M_003547"
     CDS             complement(4039..4413)
                     /locus_tag="ACHY3M_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318385.1"
                     /translation="MKDIDTETRNNTVAGDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4469..4981)
                     /locus_tag="ACHY3M_003548"
     CDS             complement(4469..4981)
                     /locus_tag="ACHY3M_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFS4318386.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFVGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4981..6165)
                     /locus_tag="ACHY3M_003549"
     CDS             complement(4981..6165)
                     /locus_tag="ACHY3M_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFS4318387.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6323..7432
                     /locus_tag="ACHY3M_003550"
     CDS             6323..7432
                     /locus_tag="ACHY3M_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFS4318388.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKRGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7658..9160
                     /locus_tag="ACHY3M_003551"
     CDS             7658..9160
                     /locus_tag="ACHY3M_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFS4318389.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAIKYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            9404..9664
                     /locus_tag="ACHY3M_003552"
     CDS             9404..9664
                     /locus_tag="ACHY3M_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFS4318390.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            9855..9995
                     /locus_tag="ACHY3M_003553"
     CDS             9855..9995
                     /locus_tag="ACHY3M_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4318391.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(10302..10601)
                     /gene="ihfA"
                     /locus_tag="ACHY3M_003554"
     CDS             complement(10302..10601)
                     /gene="ihfA"
                     /locus_tag="ACHY3M_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFS4318392.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10606..12993)
                     /gene="pheT"
                     /locus_tag="ACHY3M_003555"
     CDS             complement(10606..12993)
                     /gene="pheT"
                     /locus_tag="ACHY3M_003555"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4318393.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATSDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(13008..13991)
                     /gene="pheS"
                     /locus_tag="ACHY3M_003556"
     CDS             complement(13008..13991)
                     /gene="pheS"
                     /locus_tag="ACHY3M_003556"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4318394.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRSMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(14201..14325)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(14275..14319)
                     /gene="pheM"
                     /locus_tag="ACHY3M_003557"
     CDS             complement(14275..14319)
                     /gene="pheM"
                     /locus_tag="ACHY3M_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFS4318395.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(14442..14798)
                     /gene="rplT"
                     /locus_tag="ACHY3M_003558"
     CDS             complement(14442..14798)
                     /gene="rplT"
                     /locus_tag="ACHY3M_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4318396.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(14851..15048)
                     /gene="rpmI"
                     /locus_tag="ACHY3M_003559"
     CDS             complement(14851..15048)
                     /gene="rpmI"
                     /locus_tag="ACHY3M_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4318397.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(15145..15687)
                     /gene="infC"
                     /locus_tag="ACHY3M_003560"
     CDS             complement(15145..15687)
                     /gene="infC"
                     /locus_tag="ACHY3M_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4318398.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(15691..17619)
                     /gene="thrS"
                     /locus_tag="ACHY3M_003561"
     CDS             complement(15691..17619)
                     /gene="thrS"
                     /locus_tag="ACHY3M_003561"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4318399.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            18143..20036
                     /gene="espL1"
                     /locus_tag="ACHY3M_003562"
                     /pseudo
     CDS             18143..20036
                     /gene="espL1"
                     /locus_tag="ACHY3M_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT         5153 a         5257 c         4592 g         5034 t
ORIGIN      
        1 gttactacca atttttactc ttgaaccagg catgtcacca tctatctgtc caatggtagt
       61 attctgtcgt aacaccagat tttcaccagc atcaacagca aaatgaacaa caattccagc
      121 atgatgggga attgttaacc cttttccaat atttgcgccc aatccaattt cacaaccaaa
      181 tttgttaatt attttactgt ttaacttttt ggctgctttc ttatgtaatt tattaccatt
      241 aatatacatt tcgttagcca accgccacca gaaaaggaaa ttccggttac gctgtttttt
      301 ctctcttaaa agcctccaga tatccatacg tttccgccga attacttcat atttccagaa
      361 gttttttaaa ttagtagagt ccccaaataa aacaaagtga attgccatta agtaagacag
      421 cacgataatc tccttaatta ttatttcaga ccacacatat tataaggtta agagattata
      481 aaatcctgtt gtttgttatt caaaaacaat tttctgagaa ggacatacaa cagcaagtcg
      541 ccagtcacct tcatcaggaa attggcgaca tacgttaaat cagagcagcc ccttaactga
      601 gctggtcgcg ctattaaggg atgatgtcac cttatctttg aagccggaca acatatcgct
      661 gaacgatgag gattgcaggc gctcccgcaa atcctcatca cagcgttcaa gagtcagtga
      721 aaattctatc tttttcgcct taccgtagcg atcaaactcg gagcgggtcg tattcgttcc
      781 ggtcaggaca tacatgccgt aaatctgccc gacaccatca atcagaggcc aggggcgtcc
      841 tgtatatgcc tgcgtggtca gcagagacag cgacacttcg ccacctgtaa tttcaggata
      901 aagcacgccg gaaagcacga tgcgatcatc acctgcaccg atatactgcc agcttgctga
      961 acggttaaca cgttcatttt tcacatgccg ccagcttttg ttttgctgta actgctgatg
     1021 cggcagtgtg cgcagctcaa aaacaaacat gccgtagatc atcatcatga ccatgactcc
     1081 tcaatcttta tcgtaaaaac tgccacgtcc ggcacgggcg cgccgttcca tctctgccct
     1141 gaccatttca ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa cgttatgcag
     1201 atgaacatga atttcaccgc caaatccgga ggcaacaggc tcccggttac gggaagttac
     1261 aggaactgat gccactggag atcgtatggc ctccgccacc gggcgggagc tggccgcaac
     1321 aacagggacc agcgccggag gcagcggagc cgggaccacg ggtgtgatat taattgcggg
     1381 ggcaggctta ctgacctgcg caatcttccg ctcctgccac tccccacgaa cagcaagtgc
     1441 gcggggcagg ttcttaaaga caatatcgcc agggccaatg cgttttttcg tctcatcaac
     1501 cagcttacct gtgttatcag caattttgct gagtctgcgt agcgtcccgg tattgctgtc
     1561 tgtgagcggt ttgttgtctt tgggtttatc acctccggtg ccattgccat tttccacagg
     1621 cttcggcgga ttgattttcg ccaggtcccc ctgaagtaag gcaaccttat cctgaagaat
     1681 ggccgcacgc tgtgcgtctt cgattttctt gcgcgccctt tccgcttcat ccggaaggac
     1741 gccaagtttt tcaagtatcc acgccagcgt atccagtagc atttttgcag gtgtcagaac
     1801 aagttgtaac gcaccgccaa gaacgttacc gaatatctcg ccagcactgg tacatttatc
     1861 cagcgtttcc ttgctggact ccatcggtga cagcagcgat ttaaaccagt taaacacctg
     1921 gctgatcccg cttccgattg cgtcaaaaac agggccaaac cgttcaaagg tttcacgcaa
     1981 cgggttcagc ctttccataa tcccgctgaa caccccggca aaaaatgccc tgatgggatc
     2041 ccagtatttc cagataagga cggcagctcc ggcaagcgca gccacgataa gaccaaccgg
     2101 actgaacagc gccccgatag cgcctcccag taaagaaacg gaacccgtca ccattcccca
     2161 tagtgctggc aggaccctga cagcattcat tgatccggtc aggagagaaa aaccaagacg
     2221 cagttttgcc agcggaccag caagcacacc aatagccagc gacaacgagc caaccgttgc
     2281 agtcattgcc agcaacgcac cgcctgctat cagtagctgg cgcgtcagtg ccggatgggc
     2341 ctgcgccagc gccgtcaccc ttgataccac ccgcgtgagc cactgcgtga cagaacgcag
     2401 cggaccgtca atcagatctg caatgcggat gcgcaaccct tcccatgcac tgccgagtga
     2461 tttcagatcg ccgtcaaggt tgttggccat aacctttgct gtgcgttcag cctcaccgcg
     2521 cgcgccttca agttcttttc tcagtttggg taaggaaccg tcacccgctg catcaacgag
     2581 cgccataaac gatgtgaaag cctcttctcc ggcaatgtcc ttaaagaacg atacccggtc
     2641 aacttccccg tatttgcggg tggctttata aaggtcggcc agcacatcct ccatcgggcg
     2701 cattttgccg ttcgcgtcag agactgccac accaagctct ttcagcgcct ctgctgccgc
     2761 ctttggcggt gatgccagac gagccaggct ggcacgcatt gccgtcccgg catcacttcc
     2821 cctgataccc atattcgcca gcacgcccgc catcgctgcg gcctgctcca gcgatattcc
     2881 cagcttgccc gccaccggac ctgcatattt catggtttcg cccagtgcgc gaaggtcagt
     2941 gttggtacgg gtaaacgctg cggtgagcgt gtcaccgacc cggtccatct ggtcggcaga
     3001 aaggccgaac tgcgtcagga tgtttgagcc aatatccgcc gtctcgccga gatccatacc
     3061 gccagccgtt gccatgctca gcacaccggg aagcgcagcc tgaatggcct gtggtgtgaa
     3121 gccagccatt gcaagaaatg cctggccact ggcggcatcg cctgcggtga actgcgtttc
     3181 agagccaagt tttaacgcct gctcacgcag cgccttaaac tgtgggctgt tctggtcgat
     3241 tcgcgtcagc gcctgaacgc gggacatctc tttcccgaac ccgatcgcag gctgcaaaaa
     3301 acgcccggca gcatagccgc ccgccgctgc cgcaccaatt gccagcgcac cacctgtttt
     3361 cagttttccc gcggtttcct gcgcgcgcga ataccgctca cgcgcccgcg ttacacgcgc
     3421 aagcgcctgc cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc tgatggcctg
     3481 ctggatggtg ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagtt ctccgccaag
     3541 ttcccgcatt ttctgaattt cccgtgtgcg cgattcattc aggcgttcaa gccgggtgct
     3601 taactgctgc atcagctttt gttgtttttc gctgagcact gtacccgtgc gttgtaactg
     3661 attaagggcg ttaagctggc gtcgtgcttt cacgataccc gcatccgctt tactgacagc
     3721 gtcgcgggcg cgctcaaatg aacgcgcctg acgctcgaga tttttgatcg ccccctgcgt
     3781 tcgctggatg gagtcaccaa actgccccat caggcggcgg gcgttttcgg caggccgggt
     3841 cagcctgtca acggcgctga aagcgacccg gatatcaaga gtcttcatta tctgcattcc
     3901 cgctgcgaag tgccgcccgc tcgcgccagc taaccacttc gccgggcgtc atcatgaaga
     3961 tttcggcggg cgaccagtta aaaatggcgg caatatccgc caccagatct tcgatgtgct
     4021 caaagcacac cagggtgatt acgctgccgt ctcctgcacg ctcttcgcgc cagagtctgg
     4081 ctcgctcata aaatttacag ccactgcgca caactgaata aaatcgcgtg acgacatttt
     4141 tttaatcatc acttcatcca gtcgtggcga ggtcacgcga ggcaacagcg taaacatggt
     4201 atccgctttc agattcagca catcagacag cgacagacca cgcagggatc cagcctgctc
     4261 aatagccccg gtgatctcca catacgtgat tttttcgcca ccacgctcaa ttggtcgggt
     4321 cagttttacg ccacgttcga cagccatatc ctcacctgcc gtcacatcac ccgccacggt
     4381 gttattccgg gtttcagtat cgatgtcttt catcagttgt ctccttttca gtcagaggcg
     4441 acgcactgcg ccgcctgcat attacttatc agccaagccc aagcgcggaa cggatacggt
     4501 caggcacaat gtccttgccg tccttccggt aaatgaagtt cagcaggtca atctcccaca
     4561 acgggcgatc gttaacactc agcttgtagt aggtgttttt aatggcgtaa gtgtgtgatg
     4621 tggcttcgcc ctgtttggct tcccccatat caatttccgt cacacgcccg cgcatctcga
     4681 tttcatacag atcgctttct gcatcggtgt agtattcacc cacaaaacgc agcagcgtgc
     4741 cgtcaatcgt gccgccatac ttaaggaaca gcgcacgaac agctcccccc atgacaaaac
     4801 tcgcatcaag cgcggagtcg tccagaccga gatcaatact taccgcaccc atcatgccgc
     4861 ccccccggta gctgtcggtt ttgcgcgtca gtttgggcag agtgacggac gtcaccttac
     4921 ccacttcgtt ttcaccatcc acaaacagcg taaaaaagcg aagatgtttt ggtacagcca
     4981 tcaggcacct cccagcaccg caaatgcggg ttcaaagtat tcatcagtaa acgtctggta
     5041 aagctccatg tcttccagtg gcggaacggg cgtatatttg tagcgaatac gcacacgccc
     5101 ctgacgtaaa tccgtggtgc tgttatccac cacgtcatac cagcactccg cgccaatcag
     5161 tttcccggca gtaaccagtg aatccagttt tgccctgatg gcactgataa catccttcac
     5221 gttcgcaggc gtcagtggac tgtcgatggt ttcaaactgc gcttccgcaa ttgaatcagc
     5281 aagcacctgt gcggttcggg tatacacctc aaagatgtag gcgttcgttt ccggtgtgcg
     5341 gttgccccag aagcggaacc cgttgcgacg aataatggtc gtgatttctt tgttgttgag
     5401 gctgttggca tcgctgtctt cggcctgcaa cgaccagaac acatgcctcg acatccccag
     5461 cacattttta accggaacgt tggacagtga tttgtgccag ccctgctcat ggtcaatgta
     5521 cgcacgaagg ccgcacgcat aggcaggcgc ggggaacgtt tcgtttttgc cacttttcgg
     5581 gttgtaggcg atgaagtccg gccataagag catcacctca cgttcgttga atttctggcg
     5641 gtaggtaatc gcctcagcca tcgtgttaca gccgtgacat gaggcataca caaacgcgcg
     5701 cagtttaccc gcaatcacgc acagggattt tgttaccgcc tccgtgtcca gctccggcgc
     5761 ggccagaata cgcggacggt atccgatgct ttcatcctgc tctgcaacaa gcagcgcata
     5821 catccccgta tagctgccgt cagattcaga accaccgata accagttgat cctgcgtttt
     5881 tccgtcttct tctttatgtt cagccacgcg aacgacgatc acctttgtgc tcacctggtc
     5941 tgcgatggcc ttaagcgcac gataaagcgt ccccgttgtt ccgcattttc ccagcacgtc
     6001 attgacgcgg gtcagcagtg tgggcttgtt cagcgggaac agctccgcgt ccgcatcatc
     6061 cgccgttgcc acgataccga taacactgga atcaacatca ttaatcgctg ttaccaggtc
     6121 ggtactttcc gtaacacggg caccatgaaa acgagtttca ctcatagctt cagccccttg
     6181 tatccgttaa atgattcggc aacaatcatc acccaccacg cgcgtaatct cacccctgcg
     6241 ccgttctccc gccccggcga caacaaaaag cagtaacccc ctccgcacgc acatgcgacc
     6301 atgccgcaca gggagggagc agatgaccga caccaccatg caattgctca gtcagggcac
     6361 agaccccgtg aaaatgccgg attttgatat tctcgcggag ggtaaaacgc tgtcaggcgt
     6421 ggcagagcgc ctgatgagcc tgtcactgac cgacaaccgg ggatttgagg cggaccagct
     6481 caccatcacg ctggatgatg cggatggtca gttgcagcta ccgccacggg gcgcgcgcct
     6541 gacggttctc attggctgga aaggagaacc gctgacagaa aaaggcactt acattgttga
     6601 tgaaatcgct cacgaaggac cgccggacag gctgactgtt tcagccagaa gcgcagattt
     6661 tcgggatgaa tttaacgtta aacgtgaggt gtcctggcat gatgtgaccg ttgagcgtgt
     6721 ggtatccgcc atcgctcatc ggtatggtct gaaaccgcaa atcagcgaaa tgctgatgga
     6781 tatcgaaatc gaccacgccg accagaccga agaaagcgac atgtccttcc ttacgcgcat
     6841 ggcggaaatg ctgggcgcaa tcaccacggt aaaaagaggt aatctgttat tcatcatgcc
     6901 cggcggtggc gtgaacgcac agggccagcc gttgccctcg ttcgccatca cacgcagcag
     6961 cggcgatcgc catcagttcc gcattgctga ccgcgaagcg tatacggggg tacgcgctta
     7021 ctggcttgat cttaattacg ggaaaaagaa aaaagtcagc gtgaaacgcc gcaaaccgtc
     7081 aaaaccaaaa aaggagaaaa gcagcagccg ggaaggcgac tatatggaag gtgcggaagg
     7141 caacgtgttt gtgttacgca aaacttatca gaacgagcag gcagcaagac gtgcagcggc
     7201 ggcaaagtgg cagcagctac aacgcggagc cgcatcattc tccatcacac tggcgcatgg
     7261 acgtgcagaa ctctaccccg aaatgcatgg cacggtaaca ggatttaaaa gcgagattga
     7321 taatcaggac tggattattg caaaagccga gcacaccatt gataacagcg gctttaccac
     7381 gcagcttgag cttgaggcaa aaatcccgga atggatagca gaaacagagt gagcaactta
     7441 aatgtattaa ctcagaacgg atcaacacac tcacaacata gagtcaatat agctgctcgc
     7501 tttgttccag caaggatgcc tatttctaag gaaaaacaga gcagaaacat atggtgtttt
     7561 cctacctgac atcaggggtg gtaagaatca tgctttgata accaattgat aactattatt
     7621 atattaatct aacaccattg acatagttga ggtttgaatg aacaaaaagc tgtatttatt
     7681 cccagactca aacatttttc tacaatgcaa ggacttgacg caggtaaatt tttccgagat
     7741 tactgcttgt gatgaagtat gtatcattat tacaaggccc attcagcaag agattgaccg
     7801 ccagaaaggg caaggtaatt cccgcctatc caaaaaagca aggaaggctg ctagcctatt
     7861 taatcaagta gttgattcaa ctgacatgac tcttgttatt cgtgaacgct cgccgcgagt
     7921 ttttcttacc atggatctaa gcctgaaacc atgtgaacag ttatctgaac aattggatta
     7981 tcaggaggcc gacgatcgtt ttgttggtat tgcggcaggg tattgcgctg acgatccaga
     8041 ttcggaggtg gcaatcatta ccaatgatag cggtccacgt ttttcagcaa taaaacataa
     8101 tataatctgc tataaagtgc cttcttcatg gttgcttcct gctgagccag atgaaaagga
     8161 taaacaaatt aaattgctgg aagctaagct gaagcaattc actcagtcta tgcccgattt
     8221 cagcattaaa gtagccgatg acgagcaatt aaatattgta cttccttatt atacagcttt
     8281 atctgaatat gaagttgata atttgctttg tcaactaacg gatgcattcc cattggttac
     8341 aaactttgac aatgaacaaa ctaccccaca atcactagaa aaaatcatca agtcatttag
     8401 tcaacaggag tatttccctg tatcagcaga tgatatttct ctttataaag aaaaacatta
     8461 tccactttgg aaaaagcagt gtgaagaaat actgaaaaac tgtcatactc atattaatcc
     8521 gaaggcaata aaatacccgc tgtcgttttt attacaaaac acaggttata ctatcgctga
     8581 acgtgctgtg gtaacattta cagcaaaagg agattttcgt ctgtgtggat ttaattggga
     8641 gataatggaa gagctgactg aaagcaaaag gattgaccta ccttcagccc ctaccgcccc
     8701 caaaggaaaa tggcgtagtg tatgtggcgt aacagaaaga atgccttacg ctgggggagc
     8761 tttggaaaca ccgctatcca gtataatttc aaaagaaatt ggtcctttgt tatctcttac
     8821 tccaacagaa agggataaaa acgattttta ttataaagaa aagagaaatg agccagttca
     8881 agttgtatcg ctggaatgcg aagagtggcg tcatcaagtc aaagaagaaa tattcccgtt
     8941 gttcgtatat acagccaaca aaccaaagaa agtgaatggt gcaattgagg tgagagttga
     9001 tgcaaataac cttaccaatc cggtcattaa aacatttaaa ttaaaaataa ctatagaaga
     9061 acatagtgca ttttcaacca taacagaaat ggtgcagaca tacattcaag agcagaagaa
     9121 aagacagaaa agttctatta gcgcacaaat caaaacatga gggtgtactc aaataataca
     9181 atgctatagt aaatctataa gtgcataact atttcctgat cttttcccaa aatagatgac
     9241 aggataaaaa acattccaat cttttacagg ctttggataa atcgtatcaa agcctatttc
     9301 ttcaagagtt aaatcaccgc ttttcgctcg aaacggactg gctgttggag tatgagccag
     9361 aacaacttag aatagcccct gcactacgtt aagggaggtc gctatgttcc gttgtccgct
     9421 ttgtggcgca tctgcccgta tccgcaccag tcgtccggaa aatgattcaa acaccgtgcg
     9481 gcaaaagtat taccagtgta acaatctgga atgcggcgta tgcttctcaa cactggaagc
     9541 tttccataaa ttcacatcaa aacacgcctc cggcgttcac tcttcagaag atatcccgtg
     9601 gcatgatctg ccagcttcac acaggggaaa caatcagatg agcttgcctt tacctcagaa
     9661 ttaacaggca gaattgccgg agtaacaaaa aagcgataga ttacgcgcgg gtgcctttcg
     9721 gctgatggtc ggagggaata cccgaaggcc agatgtggaa aggccccgga aaacatctct
     9781 gtttaaccga ggccctaacc gcattacctt gacaagtgaa aggttagcgc ctctccggaa
     9841 aaggagcaag tgctatgtcg caaaaatcgc ttacgaccat cacgttctgc gtgacggcaa
     9901 tcctcatcat ctggatgttg cacggttcgc tgtgtgaaat acggatgagc ttctggggag
     9961 cggagtttgc ggcgttctta cagtgtaagc agtaaggaaa ccgcgacggg ggagcaatcc
    10021 cccgtcaatc ggttgccagg gtaaggccgg ataaggcacc ctatctcaca gatatgaaca
    10081 acaaacccgc agcgtaaaaa ctgcgggttt tctttttggt gccctcaccg acttgaaaca
    10141 gtgataagac tataagacaa agcccgcaat gtagcgggct tactcccctc accctccact
    10201 gaatgtggac acacagtgga acgaagcggc ataagatatt ataaatcaat cagttattga
    10261 aagaaaaaag gccgcagagc ggccttttta gttagatcag attactcgtc tttgggcgaa
    10321 gcgttttcga cccggctttt taacttctgc ccgggtctga aggtcaccac gcgccgtgct
    10381 gtaatgggaa tatcctcgcc cgttttcggg ttacgtcccg ggcgttgatt cttatcacgc
    10441 agatcgaagt taccaaaacc agagagtttc acctgttcgc cgttttccag agcgcgacgg
    10501 atctcttcga aaaacagttc aaccagttct ttggcatccc gcttgctaag cccaagctta
    10561 tcaaacagat attctgacat ttcagctttt gtaagcgcca taggttcaat ccctcaatga
    10621 tgcctggaat cgctctttta atgcctctac acatttggcg acggtagcgg caatctcctc
    10681 ttcttcgagt gtacggctgg tatcttgcag gatcaggctt atggcgaggc tcttataccc
    10741 ctccgcaaca cccttaccgc ggtacacgtc aaataagttt acgccaacta cctgatttac
    10801 gccaactttc ttacattcgg ataaaatatc cgctgcggga acgttttctg cgaccaccac
    10861 cgcgatgtca cgacggttcg ccgggaagcg agaaatctcg cgcgcctgag gcaccacgcg
    10921 gtctgcgagc ttgttccact ccagttcgaa caccagagtg cgaccgttaa gatccagttt
    10981 acgttccagt tcaggatgaa caaccccaac aaaaccaata cgttcacctt tcagataaat
    11041 cgctgcggat tgccccggat gcagtgccgg attcgcttct gcacggaact caacctcatt
    11101 cagtttaccg gtcaggtcga gaacggattc aagatcgcct ttcaaatcat agaaatcaac
    11161 ggtctctttt gccaggttcc agtgctcttc gtaacggtta ccgcaaatca caccggctaa
    11221 catcagatcc tgacgaatgc ccaacggtgc ctgagtatct ggtacgaaac gcagaccgct
    11281 ttcgaaaatg cgcacacggt tctgctgacg gttctggttg tacaccacgg ttgccagcag
    11341 gccggtccac agagaaagac gcattgctga catttcaaca gagatcgggc ttggcagcag
    11401 taaggcttca acgcctggat ggatcatctg ctgcactttc ggatcaacga agctgtaggt
    11461 gatcacttcc tgatagcctt tgtcgttgag cagcgttttc acgcgcttga gcgacaggtc
    11521 agcttcacgg tgagtaccca taatcaggct tgcctgtacc ggctcatccg ggatgttgtt
    11581 gtagccgtaa acacgcgcga cttcttcgac cagatcttct tcaatctcca tatcgaaacg
    11641 ccagctcggc gcaactgcct gccactcgtc tttgccttcg gtcacttcgc agccgagacg
    11701 acgcagaatg tcagttacct gctcatccgc aatatgatgg ccgatcaggc gatccagttt
    11761 gctacgacgt aaagtgatgg ttgcacgctt cggcagcgtt gcttcgttgg tgatatcaat
    11821 taccggacca gcctcaccac cgcagatgtc gatcagcaga cgggtcgcac gttccatcgc
    11881 tttgtgctgc agtgccggat caacgccacg ctcataacgg tgagacgcat cagtatgcag
    11941 gccatgacga cgagcacgac cggtgataga cagcgggctg aagaaagcgc attccagcaa
    12001 gacgttttgc gtttcgtcat tcacgccaga gtgttcgcca ccaaagatgc cgcccatcgc
    12061 cagcgccttg ttgtggtcgg cgatgaccag agtgtcagca ttcagcttcg cttcagtgcc
    12121 atcaagcagt accaacgttt cgccctcttt cgccatccgc accacaatgc cgccttcaat
    12181 gcgatcttta tcgaaagcgt gcatcggctg gcccagttcg agcagtacat agttggtgac
    12241 gtcaacaact gcatcgatag aacggatccc gcaacgacgc agtttttctt tcatccacag
    12301 cggagttggc gctttaacgt taatgccttt taccacacgg ccaagataac gcgggcaggc
    12361 ttccggcgct tcgactgtaa tcggcagcgt gtcgtcgctg gtcgcaccaa ccggaacgat
    12421 ttccggttca accagcggca gctgattcag cacggcaacg tcacgcgcaa caccaatgat
    12481 acctaaacag tcggcacggt ttggcgtcac gctgatttcg atggtgttgt catcaagttt
    12541 caggtattca cggatgtcgg tgccaatcgg cgcatccgca ggcagctcga taatgccgct
    12601 atgatcgtca gaaattccca gctcagaaaa ggagcacagc atcccttcag acggttcacc
    12661 acgcagtttc gccgctttaa ttttgaaatc acccggcaga acagcaccaa tggtagctac
    12721 cgccacacgc aggccctgac ggcagtttgg cgcaccgcag acgatgtcca gcaggcgatc
    12781 gccgccaaca ttcacttttg tcacacgcag tttgtcagcg ttcggatgct gcgcacactc
    12841 aaccacttca ccaacgacca cgccgtggaa gctaccggca accggttcta caccgtcaac
    12901 ttccaggccc gccatagtga tttgattcgc cagcgcatcg ctatcaatcg ccgggttcac
    12961 ccattcgcgt aaccacagtt cactgaattt cataatctat tcctgcctta tttaaactgt
    13021 ttgaggaaac gcagatcatt ttcgaagaat gaacgcaggt cggtgacgcc gtaacgcaac
    13081 atagtcagac gctccatccc catcccgaag gcgaaaccag agtaaacttc cgggtcgatg
    13141 ccaacgttac gcagcacgtt cggatgcacc atcccgcaac ccagcacttc cagccattta
    13201 ccgtttttac ccatgacgtc aacttctgca gaaggctcgg tgaacgggaa gtaggaagga
    13261 cggaagcgaa tctgcaaatc ttcctcaaag aagttacgca ggaaatcgtg cagagtgcct
    13321 ttcaggttgg taaagctgat gttggtatca acaatcagac cttccatctg atggaacatc
    13381 ggcgtgtgag tctggtcgta gtcgttacga taaacgcggc caggcgcaat gatacgaatc
    13441 ggtggctgct gggctttcat gctgcggatc tgtacgccag aggtctgggt acgcagcagg
    13501 cgggtagtgt caaaccagaa agtgtcgtgg tcagcgcgcg ccgggtggtg accaggaatg
    13561 ttcagagcat cgaagttatg ataatcgtct tcgatttccg gcccggttgc cacggtaaag
    13621 ccaagctcac cgaagaaact ttcgatacgg tcgatagtac gggtaaccgg atgcagaccg
    13681 ccgttttcaa tgcgacgacc tggcagagag acatcaatcg tttccgccgc cagacgcgca
    13741 ttcagtgcag cgctttccag ttccgcttta cgcgcattca gcgcctgctg aacctgctct
    13801 ttcgcttcgt tgataaccgc accagctgcc ggacgctctt ctggcggcag ctcacgcagg
    13861 gtcgtcatct gaagggttaa gtgccctttt ttacccaaat attcgacgcg cacattatct
    13921 aacgcggcaa catctgacgc ctggctaatg gccgccttcg cactggcaac cagttctgcg
    13981 agatgtgaca tggttttcct cattgtgtca gtggtgacac tggttcgttg gacttagagc
    14041 ctatcccatc aggctatttt acttgccatt ttggtcccgg gcagtgctcg aaattctcac
    14101 gtacttaaat acgctccggt ttctccgcgc tggccgtgtc cagactggct gcgacaatta
    14161 cacctgatga gacaggcttt ttatttttca aaacgcgcat acaaaaaaag cctccactgg
    14221 gaggctttca ggcgctgttt tccgtttctc ttctcacgcg ctagcctcct ggattcaggt
    14281 gctaaagtaa aaaaagaagc ggaaaatagc agcattcatt gcttgcgtta ccttttggta
    14341 ctcttcaaaa gacctttatt gaaaaggcta cggcgataaa agtcaatgtt ttgatggcgt
    14401 tgaaacgaaa agagggagac tagctccctc tttcaactgg cttatgccag agctgctttc
    14461 gctttttcaa ccagagcggt gaacgctact ttgtcgaata ctgcgatatc agccaggatc
    14521 ttacggtcga tttcaacaga ggcttttttc aggccattga tgaatttgct gtaagaaata
    14581 ccgttctgac gtgctgctgc gttgatacgc gcaatccaca gttgacggaa ctgacgctta
    14641 cgttgacgac ggtcacggta agcatactga ccagctttga taacagcctg gaaggcaacg
    14701 cggtatacgc gagaacgcgc accgtagtag cctttagctt gtttcaaaat tttcttgtga
    14761 cgtgcgcgtg caataacacc acgttttacg cgagccatat gtgctctcct gtatctatat
    14821 tctaattaaa aagttaaaaa cgttaacggc ttatgcgtac ggcaggcacg cgattaccag
    14881 gcccagatcg cctttggaaa ccatggcttt cggacgcagg tgacgtttac gtttggtcgc
    14941 ttttttggtc agaatgtgac gcaggttagc gtgcttgtgc ttaaaaccac ctttaccggt
    15001 ttttttgaag cgcttagcag caccgcgtac ggtcttaatt tttggcattt taataacttc
    15061 cacttcgcat tgttaataaa cgaaacaaag gcgaacaaag cctgtgaagc ccgaaggctc
    15121 cacagacagt gctacttgaa ggccttactg tttcttctta ggagcgagca ccatgatcat
    15181 ctggcggcct tcgatcttcg ttgggaagga ttcgaccact gccagttctt gcaaatcgtc
    15241 tttcacgcga ttaagcactt ccataccgat ttgctggtgc gccatctcac gaccgcggaa
    15301 acgcagcgtg attttggctt tatcaccctc ttcgagaaag cgaatcaggc tgcggagttt
    15361 tacctgatag tcgccttcat ctgtaccagg acggaattta atttccttaa cctggataac
    15421 tttttgcttt ttcttctgtt ccttagaaga cttgctcttt tcatagagga atttgccgta
    15481 atccattata cgacaaaccg gcggctcggc gttagggctg atctcgacta agtctactcc
    15541 ggcttcttct gctttctcca gagcttctct cagactcaca ataccaagct gctcgccttc
    15601 cagacctgtt aagcgaactt cctgggcgcg aatttcgcca ttgatacggt tagggcgcgc
    15661 cgtttgaact cgttttccgc ctttaatacc ttattcctcc aattgtttaa gactgcggct
    15721 gcgaatctct tgttgcagct tctcgatcac ttcatttacg tccatgcttc ccaggtcttt
    15781 accacggcgg gtgcgaacgg caactttgcc tgattccacc tctttatcac cacagaccag
    15841 catatatggg acgcgacgca aagtgtgctc gcggatttta aagccaatct tctcatttct
    15901 caagtctgct ttaacacgaa tgcccgcatt tgatagtttt tgcgtcaatt cgttaacgta
    15961 ttcagactgt gaatcggtaa tattcatgat aacaacctga actggcgcaa gccaggtcgg
    16021 gaagaaacca gcgaattctt cggtcaggat accgatgaaa cgttccatcg atcccagaat
    16081 tgcgcggtga atcattaccg gtactttacg ttcattgtct tcgcctacat aagaagcgct
    16141 cagacgagaa ggcaaagaga agtccagctg tactgtaccg cactgccatg cacgatcgag
    16201 gcagtcatac agggtaaatt caattttcgg accgtagaaa gcgccttcac ccagttgata
    16261 ttcaaacggg atgttgtttt cttccagcgc aaccgccagg tccgcctcag cacgatccca
    16321 catttcatca ctgccaatac gtttttcagg acgagtggag agtttgacga cgatcttctc
    16381 gaagccaaaa gtgctgtaca tatcatagac taaacggata catccgttaa cttcatcgcg
    16441 aatttgttct tcagtacaga agatatgcgc gtcatcctgg gtaaatccac gcacgcgcat
    16501 caggccatgc agcgaacctg acggctcatt acggtggcag ctaccaaact cggccatacg
    16561 cagcggcaga tcgcgataag acttcagccc ctggttgaaa atttgtacgt gacccgggca
    16621 gttcatcggc ttaatgcagt attcacggtt ctcagaagac gtggtgaaca ttgcatcttt
    16681 gtagttgtcc cagtgaccgg ttttttccca caggacacgg tccatcatga acggaccttt
    16741 aacttcctga tactggtact ctttcagttt agaacgaaca aacacttcta gttcacggaa
    16801 gatggtccag ccgtcgttgt gccagaatac catacccggc gcttcttcct gcatatggta
    16861 caggtcgagc tgtttaccga ttttacggtg gtcgcgtttc gcggcttctt ccaggcgctg
    16921 caggtaagcg ttaagcgctt ttttgtctgc ccacgccgta ccgtaaatac gttgcaacat
    16981 tttgttgttg ctgtcgccac gccagtaagc ccctgccgtt ttcattagtt tgaaatgatg
    17041 gcagaaacgc atgttcggta cgtgcggacc gcggcacata tcgacatatt cttcatggaa
    17101 gtacagacct ggcttgtcat catgggcgat gttttcgtca agaatggaga ctttgtagct
    17161 ctccccacgg ttggcgaaag tttcacgcgc ttcgtgccag ctgactttct tcttaatgac
    17221 gtcgtagttt ttctcagcaa gctcatgcat ccgcttctcg agtgcttcga catcttcctg
    17281 ggttaacgtg cggtcaagat caacgtcgta ataaaaaccg ttgtcaataa ccgggccgat
    17341 tgccattttg gtatgcggcc aaagttgttt aatcgcgtgc cctaacaggt gcgcacagga
    17401 gtgacgaatg atctccagac cgtcttcgtc tttggcggta atgatggaca gttgtgcgtc
    17461 gttttcaatc agatcgcaag catcaaccag ttcgccatta acgcgccctg cgatacaggc
    17521 tttcgccaga cctggaccaa tgtccagcgc aacatccatg gggcttacag cgtggtcgta
    17581 atggcgttgg ctgccatcag gaagagttat aacaggcatt ttatatcctt atttgcagtg
    17641 gtgacccaca cgaaagatca catacaaaga aaaatttgtt tattaacagt tcattgcgaa
    17701 accatctagc caacaaatgc taaattggtt cgcaatcagg tacacaaatg aaagacatta
    17761 acctctcacc ttccacccgg ttgagcgatg ttaacactaa aaaaagggag attgtacctt
    17821 tccgtttcac attgattcga ttcgcaattc gtttgtagtg agtacaccag caaaacgctt
    17881 aaagaaccac tgataacatc acggtgcgtc tgaaaggttt actgcgagcg gatttacagc
    17941 agcataaact gaatccagca aagttcgttg cgtgatttgg cggtagtatt ttaaacgctc
    18001 atgagattaa tgacgaagtg gtcatatcac aatgataaaa gtgacacaat tcttataaca
    18061 atttttcgtg cacatttcgt tctggcgata ataattaatc atcatcctca aaacctccag
    18121 atatatataa ggcgaataga ttatgagtca aaacgatatc attatcagaa ctcattataa
    18181 gtctcctcat agattgcaca tcgatagcga cataccaaca ccttcatcag agcctattaa
    18241 taaatttgcg cgccagctca tcaccctact tgatacctct gacttaagtt cgatgctgtc
    18301 atactgtgtt actcaggaat ttaccgcaaa ctgtcgaaaa atatcacaaa attgttattc
    18361 cactgccctt tttaccatta accttgccac ttcacccatc catgcagaaa atatactcat
    18421 tacattacac tataaaaaag atatcatttc cttattactg gaaaccacgc ctattaaagc
    18481 taaccatttg cgaagcatac tggattatat tgaacaggaa cagttaactg ctgaagatcg
    18541 taaccattgt atgaaactgt ctaaaaaaat ccatagagaa aaaactatac acccaacagt
    18601 aaatctcaat ggtagtgcat gttttttgca atctccttct gacgctattt tttgtcgcca
    18661 tctgtcattg caatacgccc ttgattcatt gagaaatgga aaaggcaaag tcaacctgat
    18721 taaacattac tcctccgttg aatccataca gcagcatgtc cccttagtcc gggacgcgga
    18781 gttcagagca ttacttcgcc atcctcctgc agggagtcgc gttatcgcga gtaaggattt
    18841 tggcttcgct ttagattttt tcttttgtcg aatgatggca aacaatatca gtcatatgtc
    18901 cgcgatttta tatatagaca atcatacttt gtcagtaagg ctacgaataa agcagtcagt
    18961 gtatggacaa ttaaactatg ttgtgtccgt ttacgacccg aacgatacca acgttgccgt
    19021 cagagacacc cacaggacag cacggggctt tctttcgctt gataagttca tcagttcagg
    19081 tcccgatgct cagacctggg ctgataggta tgttcgcaac tgtgcaattg ctattctgcc
    19141 cctattacct gtgggagttc caggggctat tttcgcgggt attgcatcac gaatgccatt
    19201 tgcccctata catccatcgg caatgttgtt aataatggct acaggccaga ctcaacagct
    19261 tattacatta ttcaaacagt tgcccatact ccctgaaaaa gaaatcattg aaataataac
    19321 tgcgcagaat agcgttggta cacctgcttt atttttggct atgatgaacg gacatactga
    19381 caacgtgaaa atatttatgc aagaaattca gtcactggta gataatcaca tcattcatga
    19441 agataacctg gttaaattac tgcaaactaa aagtgctaac gaaatacctg gactctatat
    19501 ctccatgttg tatggattcg atgaaataat cgatatcttt ctgaatgcat taaccactcc
    19561 aatagcacaa gaacttttaa acaaaaaaat ggtgatggat attttagcca tgaaaacacg
    19621 tgatggtgaa ccagggttat ttgctgcaat ggaaaataat caccctttgt gtgtcacacg
    19681 gttcctctct aaagtttatg gaatcgccgt taaatataaa ctcagcaaaa tcaacatcat
    19741 ggacttatta aaaggcgcga cagcacaggg aacccctgca ttatacatcg ccatgagcaa
    19801 gggtaatgaa gacgtcgtgt tatcttatat atcgacgctg ggtgcttttg caaaaaaaac
    19861 attcttttag tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt
    19921 cagctgttca tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg
    19981 ctataaatgt catcagccaa agcctgagtt ttagtgctga tgaaataaag acgtat
//
LOCUS       DAZUFB010000089        19720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZUFB010000089 DAZUFB010000000
VERSION     DAZUFB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.089"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..168
                     /locus_tag="ACHY3M_003563"
     CDS             <1..168
                     /locus_tag="ACHY3M_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318400.1"
                     /translation="SSEELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTRED
                     YTYNYDLLNAI"
     gene            complement(563..939)
                     /gene="rnpB"
                     /locus_tag="ACHY3M_003564"
     ncRNA           complement(563..939)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHY3M_003564"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(972..2117)
                     /gene="garK"
                     /locus_tag="ACHY3M_003565"
     CDS             complement(972..2117)
                     /gene="garK"
                     /locus_tag="ACHY3M_003565"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFS4318401.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2214..3104)
                     /gene="garR"
                     /locus_tag="ACHY3M_003566"
     CDS             complement(2214..3104)
                     /gene="garR"
                     /locus_tag="ACHY3M_003566"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS4318402.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3134..3904)
                     /gene="garL"
                     /locus_tag="ACHY3M_003567"
     CDS             complement(3134..3904)
                     /gene="garL"
                     /locus_tag="ACHY3M_003567"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFS4318403.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATKKLADTFKK"
     gene            complement(3920..5254)
                     /gene="garP"
                     /locus_tag="ACHY3M_003568"
     CDS             complement(3920..5254)
                     /gene="garP"
                     /locus_tag="ACHY3M_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFS4318404.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5629..7200
                     /gene="garD"
                     /locus_tag="ACHY3M_003569"
     CDS             5629..7200
                     /gene="garD"
                     /locus_tag="ACHY3M_003569"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFS4318405.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7349..7684
                     /gene="prlF"
                     /locus_tag="ACHY3M_003570"
     CDS             7349..7684
                     /gene="prlF"
                     /locus_tag="ACHY3M_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFS4318406.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7684..8148
                     /gene="yhaV"
                     /locus_tag="ACHY3M_003571"
     CDS             7684..8148
                     /gene="yhaV"
                     /locus_tag="ACHY3M_003571"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFS4318407.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8203..9012)
                     /gene="agaR"
                     /locus_tag="ACHY3M_003572"
     CDS             complement(8203..9012)
                     /gene="agaR"
                     /locus_tag="ACHY3M_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFS4318408.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9261..10541
                     /gene="kbaZ"
                     /locus_tag="ACHY3M_003573"
     CDS             9261..10541
                     /gene="kbaZ"
                     /locus_tag="ACHY3M_003573"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFS4318409.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHCDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10564..11037
                     /gene="agaV"
                     /locus_tag="ACHY3M_003574"
     CDS             10564..11037
                     /gene="agaV"
                     /locus_tag="ACHY3M_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFS4318410.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11048..11827
                     /gene="agaW"
                     /locus_tag="ACHY3M_003575"
     CDS             11048..11827
                     /gene="agaW"
                     /locus_tag="ACHY3M_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFS4318411.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11817..12695
                     /gene="agaE"
                     /locus_tag="ACHY3M_003576"
     CDS             11817..12695
                     /gene="agaE"
                     /locus_tag="ACHY3M_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFS4318412.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12713..13147
                     /gene="agaF"
                     /locus_tag="ACHY3M_003577"
     CDS             12713..13147
                     /gene="agaF"
                     /locus_tag="ACHY3M_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFS4318413.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13144..14277
                     /gene="nagA"
                     /locus_tag="ACHY3M_003578"
     CDS             13144..14277
                     /gene="nagA"
                     /locus_tag="ACHY3M_003578"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4318414.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14628..15782
                     /gene="agaS"
                     /locus_tag="ACHY3M_003579"
     CDS             14628..15782
                     /gene="agaS"
                     /locus_tag="ACHY3M_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFS4318415.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15795..16655
                     /gene="kbaY"
                     /locus_tag="ACHY3M_003580"
     CDS             15795..16655
                     /gene="kbaY"
                     /locus_tag="ACHY3M_003580"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFS4318416.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16822..17298
                     /gene="agaB"
                     /locus_tag="ACHY3M_003581"
     CDS             16822..17298
                     /gene="agaB"
                     /locus_tag="ACHY3M_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFS4318417.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17337..18140
                     /gene="agaC"
                     /locus_tag="ACHY3M_003582"
     CDS             17337..18140
                     /gene="agaC"
                     /locus_tag="ACHY3M_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFS4318418.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18130..18921
                     /gene="agaD"
                     /locus_tag="ACHY3M_003583"
     CDS             18130..18921
                     /gene="agaD"
                     /locus_tag="ACHY3M_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFS4318419.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18922..19677
                     /gene="agaI"
                     /locus_tag="ACHY3M_003584"
     CDS             18922..19677
                     /gene="agaI"
                     /locus_tag="ACHY3M_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFS4318420.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGLTLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4796 a         5136 c         5002 g         4786 t
ORIGIN      
        1 agctcagaag agttactttc agataacacc ctcgagaaag acgaaaattc tccggacaat
       61 ggttttgagg tcggggaata taacacatat gaagcatata actcagagaa tcaatatttt
      121 accagagagg actatacgta taattacgac cttttaaatg caatatagac ttaataactt
      181 cttacaaagt ctattattaa taccgtagat atttattcat cttaccctgt gcaccaaaag
      241 caaaaactca ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta
      301 cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt tgtaggcagg
      361 ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc
      421 ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat
      481 agcgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccggtttac
      541 gctggcgggt tttcgttaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac
      601 agtcattcat ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac
      661 gggccgtacc ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg
      721 actgttacca gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct
      781 tgcgcgggcc atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg
      841 ttacctggca ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga
      901 agaggacgac gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt
      961 gcgcgccctt gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt
     1021 gaagcacggc agatattgtc ataagccccg cggaatgctt cgtccaacgt accaatgctg
     1081 gtcagtacgc tgaagacagc atcaatgcca tgctgatgta caacgccaac atcatcggtc
     1141 aggctacccg caatgccaat caccggttta tggtacttct tcgccacgtt tgcgacgcca
     1201 atcggtacct tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc
     1261 gtacaatcgt gaatatgctc ctccagattc agcgccgtag tgacgatttc aataccactt
     1321 ttcagttccg caccaagaaa cgccattagc gccgcgccca taccacccgc agctcctgcg
     1381 ccggggacat ctttcacatc aacatgcagc gcttttttaa tgacctcggc atagtgagag
     1441 aggttattgt ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg
     1501 cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg
     1561 caatctttta agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta
     1621 ccgccgccaa aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt
     1681 accatgcctg cgccgccatc atttgtagcg ctaccgccaa tgccgataat aatgtttgtc
     1741 gcgccgctct ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggtcacgagt
     1801 ggatcgcgtt tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac
     1861 gcggttttgc catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct
     1921 gtaacccagg cgtgacgttc agaaccctgg gtggctgcaa tcatcgcttc caccgttcct
     1981 tcgccaccgt cggcaaccgg aatagaaacg tactgtgcat caggaaaaat ttcccgaaat
     2041 cctttttcta tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg
     2101 gcgattacga ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta
     2161 ttcggcctgg attccaggcc cggattgcaa tacgccatcc gggcacgacg tcattaacga
     2221 gtaacttcga ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt
     2281 cctaaaccat ctgctcgcag tgcctgcatc atctccataa ccgcagctgt gagcggcagt
     2341 tgcgcgccga cgccgtggga agtatccagc gcatttgcca gatccttaat atgcagatca
     2401 atacggaagc ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg
     2461 gtactgcccg ccagtccacc gcgaattgcc tgatacacca ggtccgggtt aacgcccgct
     2521 ttggttgcca gcgttagcgc ttctgacatc gcggcaatat tcagcgccac aatgacctga
     2581 tttgccagtt tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc
     2641 atcgctttca tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact
     2701 gacagcgtac cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca
     2761 atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc
     2821 atatcgatca ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc
     2881 acctctttca catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg
     2941 atcgctttag ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct
     3001 gggttacggt cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt
     3061 ttacccataa tccccaggcc aataaaacca actttcatag tcatatcaat catctctctt
     3121 gttgcggtgg tggttatttt ttaaaggtat cagccagttt cttagtggca gagcggaaga
     3181 cgccaagatc gctgccgaca gccacaaacg tcgcacccca ttccagataa cgacgcgcat
     3241 cggcttcgac cggcgcgaga ataccgctgg gtttgccgtg cgcactggca cggttaaaaa
     3301 tgtgctgaat tgctttttgt acatccgggt gcgaggcatt gccgagatgg cctaatgccg
     3361 cagccagatc gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatggcat
     3421 cgacgttatc tacgccctgc tgactttcta tctggaccag aatggtgatg ttcttgttcg
     3481 actgagcgaa ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc
     3541 cgcgaatgcc ttccggtggg taacgggttg atgccaccgc ctgctctgct tcctcttttg
     3601 tttctacaaa aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta
     3661 ccggctcgtt ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta
     3721 actgcggaat aaacgtggag atatcgtttg gcgcatgttc gccatccagc accagccagt
     3781 caaacccagc caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac
     3841 caatttgtac ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat
     3901 tcatcgctta tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga
     3961 agaggtagca gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga
     4021 aggagtgcag ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat
     4081 tgccaaagac gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag
     4141 aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca
     4201 tgaccaccag cgtggtgttg ttggtgtagt tacataagat gatggtggaa gccagcaaca
     4261 ttcccagcac aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat
     4321 aatccgagaa gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg
     4381 cgaccagacc cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc
     4441 aggtgaggaa gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca
     4501 tcatgcggtt agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc
     4561 tgcccggctt tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct
     4621 cttccgcaga catacgtggg tgatctgtcg ggttatgaat caatttgatc cacagcgccg
     4681 tcagcacaaa accaatcacc cccataacgg taaagacgtg ctcccagccc caggcgaaag
     4741 tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga
     4801 tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg
     4861 ccgggaatga tggcgcttcc gagaagccga gcataaagcg cataaagaac atggagatcc
     4921 ctgcccaggc cagcgggaac atatcaacaa agccttgcag gaaagtgaat agcgaccaga
     4981 aaaagaggct ataggtataa acttttttcg agccaaactt atcaagcagc cagccgccgg
     5041 ggatttgcat cagcaagtag gcccagccaa aagcagaaaa gatgtaaccc atcgaaaccg
     5101 cacttaactg caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg
     5161 cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagcgg gtatgcacgc
     5221 ctttcttttt ttcgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg
     5281 atttttatta ttatggggaa ggtgttattt atgagtttca tttatgccgt aacggcaatg
     5341 aactcgggaa ttagtataag cagcgcgaga ataataatca ttgtgcaaat gctaatttaa
     5401 ttaatgctat ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag
     5461 atttgcgctt taccttgccg ggcggcactg caatccctga aatgattgac attgatcaca
     5521 tttctgcgtt taaactcctg acattcttat ttcacccaat gaagtcattt atttttaaat
     5581 gagaccaggt cctcatttta ataacccctg gctggagaat atagtacaat ggccaacatc
     5641 gaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg
     5701 gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tggactggaa
     5761 ttaattgaac atattcccca ggggcataaa gtcgcattgc tggacattcc ggctaatggt
     5821 gaaattattc gttatggtga agtgattggt tacgccgtgc gtgcaatccc acgcggaagc
     5881 tggatcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg
     5941 gcaaccaaag tcccggcccc cttaccgccg ctggaaggat acacctttga gggctatcgc
     6001 aatgccgatg gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt
     6061 gtggcaggcg tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac
     6121 ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gctgtggcgt ggcgattaac
     6181 gcaccggcgg cagttgtgcc tattcgtacc atccacaata tttcgctgaa ccctaacttt
     6241 ggcggcgaag taatggtgat tggtctgggt tgtgaaaagt tgcagcctga gcgcctgctg
     6301 gtcggcacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag
     6361 gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc
     6421 catctacaaa aactgaatca acgtcagcga gaaacctgtc cggcttcaga actggttgtc
     6481 ggcatgcagt gcggtggcag cgatgccttt tctggcgtaa cggcaaaccc ggcggttggc
     6541 tatgcgtctg atctactggt gcgctgcggc gcaacggtga tgttctcaga agtcacggaa
     6601 gtgcgtgacg cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg
     6661 ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc
     6721 gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa acgtggtaga gaaggcactc
     6781 ggctccattg ctaaatcggg taaaagcgca attgttgaag tgctgtcgcc cggtcaacgc
     6841 ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg
     6901 caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg tacgccgtac
     6961 ggcctgatgg cggtacccgt cattaaaatg gcgacccgca ccgagctggc gaaccgctgg
     7021 tttgatttaa tggatattaa cgcgggcact atcgccaccg gcgaagaaac cattgaagag
     7081 gtgggctgga agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc
     7141 tcggatcaat gggggctgca taaccagctg gcagtgttta acccggcacc ggtgacctga
     7201 tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta ttgtttaaag
     7261 cccccacgtc cattaataat gcatttgcat taccttagtt caagcttata atttgagcag
     7321 aaaacaggct gtaaaaggac agtgaatcat gcccgctaat gctcgctctc acgctgtact
     7381 gaccactgaa tcaaaggtca cgatacgcgg acaaacaact atccccgcac cagtgcgtga
     7441 ggccttaaaa ctgaagccag gccaggacag cattcattac gaaattctgc ctggtgggca
     7501 agtctttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg catttttgcg
     7561 ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattca acattcaaca
     7621 aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg gcgacgacga
     7681 ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc ctgttttcag
     7741 gaaacctacg acgctttagt tgccgaagtc gagacattaa agggaaaaga tcctgaaaat
     7801 tatcagagaa aagccgccac aaagttattg gcggtagtcc ataaagtgat tgaggagcat
     7861 atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg ctctgggaaa
     7921 aataaagact ggtcacgggt aaaatttggt gctggtcgtt atcgtctctt ctttcgttat
     7981 agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac tctgcgcacc
     8041 tacggtaaaa aaacagatgc ctataccgta ttcagcaaaa tgttaaaaag aggacatcct
     8101 cctgccgact gggaaaccct cacccgagaa acagaagaaa cccattgatg gtgttcacaa
     8161 tgcccgccga acgtattcgt gttgaccagc gggcaccctt tctcactccc cgaccagaat
     8221 cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc cttcatcaac
     8281 aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac gattgaattt
     8341 actggaatcg gtgaccacga tgatccgttc cacaacttcg cacatccgac ggtttaaacg
     8401 ggcttcatct tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag
     8461 aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcgc cgtaaaaaga
     8521 ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt ccgcttccag
     8581 caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt gcttacgcat
     8641 cagacgagca atctcaaaag tggtagtccc ggaatcgagg atcacccgat gaccaggctg
     8701 aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca gcgcgctttt
     8761 atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg cgccaccata
     8821 ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcattgcgga tcgtcacagt
     8881 agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct gtcgcagacg
     8941 ctgaatgatc tgttctcgtc gctcgctggt gccagtcact cgcttctcac ctgaagcgtc
     9001 ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt tgcctattaa
     9061 cgcctttcta ttaagcaaat gcaagcccac cttgcccatt gacgcaagct actctcgttt
     9121 cagtgacttt cattatgttt cttttgtgaa tcagatcaga aaactattat ctttcgtttt
     9181 atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta atatcgcagt
     9241 aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg
     9301 gcaaaacaaa tggaatttat gccgtttgtt ccgcacatcc gctggtgctg gaggctgcaa
     9361 tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc aatcaggtag
     9421 accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc
     9481 tcgccgactc gttgaatttc ccgcaggatg cgttgattct gggtggtgac catctggggc
     9541 caaaccgctg gcaaaacctg ccggccgctc aggcaatggc caatgccgat gatttgatta
     9601 aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg tcctgtcagg
     9661 acgatccgat tcccttaact gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag
     9721 tggcggaaga aacctgtctt gaacactttg gcgaagccga tctggagtat gtcattggta
     9781 ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc
     9841 cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag caaggtttga
     9901 atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca
     9961 ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg gtcgaaaact
    10021 acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa tcgctgcgcc
    10081 agctggtgat tgaccacttt gccattctga aagttggccc agcgctgacc ttcgccctgc
    10141 gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagcg aaagcctgtt
    10201 ctggtctgcg tcaggtgctg gaagacgtga tgctcgaccg cccggaatac tggcaaagcc
    10261 actaccactg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac tcggatcgcg
    10321 tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg gttcgtaatc
    10381 tggcggattc accaattccg ctaccgctca tcagccagta tctgccgctg caatacgtga
    10441 aagttcgctc cggcgagctg caaccgacgc cacgggaact cattatcaac catattcagg
    10501 acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa agaggaacac
    10561 gctatgccaa atattgtttt aagccggatt gatgaacgct tgattcacgg tcaggtcggc
    10621 gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga tgaggttgcc
    10681 gaagatccgg tacaacaaaa cctgatggaa atggtactgg cagaagggat cgccgtacgt
    10741 ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg acagaaaatc
    10801 ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg cgttccggtg
    10861 aatcgcatta acgttggcaa tatgcactac gccaatggca aacaacaaat cgccaaaacg
    10921 gtttctgtgg atgcgggcga tatcgcagca tttaacgacc tgaaagccgc tggggtggaa
    10981 tgcttcgttc agggcgtccc gacagagcct gctgtagacc tctttaaatt actttgaggg
    11041 attcatcatg gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg cctttatcgc
    11101 tggcctggat atgtttaacg gcctaacaca tatgcaccgc ccggtggtcc tcggcccgtt
    11161 ggtcgggctg gtacttggcg atctgcatac cggaatttta accggcggta cgctggaact
    11221 ggtgtggatg gggctggccc cgctggcggg cgcacagccg cctaacgtga ttatcggtac
    11281 tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg cggtcggtgt
    11341 cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct cggtgatgtc
    11401 tggcgtgatg tctcgctgcg accggatggc ggagaacgcc gatactcgcg gcattgaacg
    11461 cgtgaactat ctggcactgc tggcactcgg cactttctat tttctctgcg ctttcctgcc
    11521 tatctacttc ggcgcggaac atgcaaaaac catcattgat gtcctgccgc aacgattaat
    11581 cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg tgctgctgaa
    11641 aatcatgatg aaaaacgtct acatccccta cttcatcctg ggctttgttg ccgcagcctg
    11701 gctcaagtta ccggtgctgg ctattgctgc cgctgcgctg gcgatggcgc tgatcgacct
    11761 gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat ttgaagatgg
    11821 catctaatca aaccaccctg ccgaacgtca gtgaaaacga agaaacattg ctgactggcg
    11881 tcaatgaaaa cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa aaagatatca
    11941 accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt taactacgaa cgtatgcagg
    12001 cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa gatccacact aataaacgcg
    12061 acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg tttctggtga
    12121 catttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt aacagtattc
    12181 agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat tggcgatgca atgttctggc
    12241 taacgctact gccgatttgt ggcgggattg gagccagtct ggcattgcaa ggctccattc
    12301 ttggcgcagt cgtctttatt ctgctgttca acgtggtaca cctggggctg cgttttggtc
    12361 tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc actcattaaa gcgaatacca
    12421 aaaaagtcgg ccatgctgcg tctatcgttg ggatgacggt aatcggtgcg ctggtggcaa
    12481 cctatgttcg tttaagcacc acgctggaaa tcacagcggg cgacgcagtg gttaagttac
    12541 aggctgatgt tatcgacaaa ctgatgcctg ccttcttacc gttggtctac accctgacca
    12601 tgttttggtt ggtacgccgc ggctggagtc cgctgcgtct gattgcagtt accgtggttc
    12661 tcggcatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt agatgttaag
    12721 tattattttg acagggcatg gcgggtttgc cagcggcatg gaaaaagcaa tgaagcaaat
    12781 tctcggtgag caatcgcagt ttatcgccat cgattttccg gaaacctcaa gcaccgcgct
    12841 gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg gcattgtttt
    12901 tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg cgctgcaaaa
    12961 accgggctgt gaagtgatca ccggcaccaa tttgcaactg ctactggaga tggtgctgga
    13021 gcgcgaaggg ttaagcggtg aagaattccg tgtacaggcg ctggagtgtg gacatcgtgg
    13081 gctgaccagt ctggtggacg agttaggccg ctgccatgaa gaatgtccgg tcgaggaagg
    13141 gatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg gctcgatgac
    13201 catcagttgc gtattgctga cggtgtcatc gcagcaatcg aaccgattcc agtgggcgtg
    13261 actgaacgcg atgcggaact gctctgcccc gcttacatcg acacccatgt acacggtggt
    13321 gcgggcgttg atgtaatgga tgacgcgccg gatgtactcg acaagctggc aatgcacaag
    13381 gcacgcgaag gtgtcggcag ttggttgccg actaccgtaa ccgcgccgct taacaccatt
    13441 catgcggcgc tgaaacgtat tgctcaacgt tgccaacgcg gcggacctgg tgcgcaagtg
    13501 ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc gcatccgccg
    13561 gaattgtttc gcgagcttga aattgccgag ctggatcaat tgattgccgt ttctcagcac
    13621 accttacgcg tggtagcgct ggcaccggaa aaagaggggg cattgcaggc catccgccat
    13681 cttaaacagc aaaatgtacg agtgatgctg gggcatagcg cggcgacctg gcaacaaact
    13741 cgcgccgcgt ttgatgctgg tgccgacggc ctggtgcatt gctataacgg gatgacaggt
    13801 ttacatcacc gcgaaccggg aatggttggc gcgggattaa cggacaagcg cgcctggctg
    13861 gaactgatag ccgatggtca tcatgtgcat ccggcggcga tgtcgctgtg ttgttgctgt
    13921 gcaaaagaga gaatcgtgct gatcaccgac gcgatgcagg cagccgggat gccggatggt
    13981 cgctatacgt tatgtggcga agaagtgcag atgcacggtg gcgttgtccg taccgcgtcc
    14041 ggtgggctgg cgggcagtac gctgtctgtt gatgcggcag tgcgcaacat ggtcgagttg
    14101 acgggcgtaa cgcctgcgga agccattcat atggcatcgc tgcatccggc gcgaatgctg
    14161 ggtgttgatg gtgttctggg atcgcttaaa ccgggcaaac gcgccagcat cgttgcgctg
    14221 gatagcgggc tgcatgtgca acaaatctgg attcagagtc aattagcttc gttttgatat
    14281 tttgctcctt tattgggcct tcacttcccc cgtaaggcct ttctttttct ttcgttttga
    14341 tctgtgcagc ggtgtcggat gcgacgctaa cgcgtcttat gcgacctacg gtacgccgca
    14401 tccagcaatc ggtgaacata gcgacgtcat tacgcaattc tttaatctcc ccttgtcttt
    14461 tctttcaatg atcacagatt tcgttttatt tcctttttct ccattgaact ttcggtttct
    14521 tttctataga ttttaatcaa cgaaagacat caccaagtga aatgaaacga aaggcaagtg
    14581 aaagcgacaa cgcccgacgt caagttcatc agactaagga ttgagttatg ccagaaaatt
    14641 acacccctgc tgccgccgca accggtacat ggactgaaga agagatccgc catcagcctc
    14701 gcgcatggat ccgttcactc accaacatcg acgcgctacg ttctgcgctc aataacttcc
    14761 ttgaaccgtt actgcgcaaa gagaatctgc ggatcatcct gaccggagcc ggaacctcgg
    14821 catttatcgg tgacatcatc gcgccgtggc tcgccagcca taccggtaaa aacttcagcg
    14881 ccgtaccgac caccgatctg gtcaccaatc cgatggacta cctgaaccca gctcatccgc
    14941 tgctgttgat ctccttcggt cgatccggca acagcccgga aagcgtcgct gccgtggaac
    15001 tggcaaatca atttgtacca gaatgctatc acctgccgat cacctgcaac gaagcgggcg
    15061 ctctttacca aaacgcgatc aacagcgata acgcgtttgc cctgctgatg cccgcagaaa
    15121 cgcacgatcg cggctttgcg atgaccagca gcattaccac catgatggcc agctgcctcg
    15181 cggttttcgc acctgagacg atcaacagcc aaaccttccg cgacgtggcg gatcgttgcc
    15241 aggcgatcct gacctcactg ggcgatttca gcgaaggtgt gtttggttac gcaccgtgga
    15301 aacggatcgt ttatctcggc agcggtggct tacagggcgc agcacgcgag tcggcgctga
    15361 aagtgctgga actgactgcg ggtaaactgg cggccttcta tgattccccg accggattcc
    15421 gtcatggccc gaaatcactg gtcgataacg aaacgctggt ggtggtattt gtctcaagcc
    15481 acccttacac ccgtcagtat gatcttgatc tgctggctga actccgccgt gacaaccagg
    15541 caatgcgcgt aatcgccatc gccgcggaaa gcaccgacat cgtcgctgcc ggtccacata
    15601 ttatcctgcc gccgtcacgt cactttatcg acgttgagca ggcattttgc ttcctgatgt
    15661 acgcccagac gtttgcactg atgcagtcgc tgcacatggg caatacgccg gataccccat
    15721 cagccagcgg caccgttaac cgcgtggtgc aaggcgtaat cattcatccg tggcaggcat
    15781 aagaggatcg cattatgagc attatctcca ctaaatatct gttacaggac gcccaggcca
    15841 atggctacgc ggtgcctgct tttaacattc ataacgccga gacgatccag gcgatccttg
    15901 aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg aacgccgggg acctttaaac
    15961 atatcgcgct ggaagagatc tacgccctgt gcagcgccta ttccacaacc tacaacatgc
    16021 cactggcgct gcatctcgac caccacgaat cgctggatga tattcgccgt aaagtccacg
    16081 caggtgtgcg cagtgcaatg atcgacggca gccacttccc gtttgccgag aacgtgaagc
    16141 tggtgaaatc ggttgttgac ttctgccact cacaggattg cagcgtggaa gcagaactgg
    16201 gccgcctggg cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca ttcctgaccg
    16261 atccacaaga agctaaacgc tttgtcgaac tgactggcgt cgacagcctg gcggtagcga
    16321 ttggtacggc gcacggctta tacagcaaaa cgccgaagat tgatttccag cggctggcgg
    16381 aaattcgtga agtggtggat gttcctctgg tgctacatgg tgccagcgat gttccggatg
    16441 aatttgtccg tcgcactatt gaacttggcg tcacaaaagt gaacgttgcc acagaattaa
    16501 aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga aaatccgcag ggtaatgatc
    16561 ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga agttgtcaga aataaaatta
    16621 atgtctgtgg ttcagcgaat cgaatttcag cataatcatt agttttttat gatttatccc
    16681 aatgtacttc ccgattaata tcggggagtg ccttaatgga aaaggagatg actaaacctt
    16741 aataaataca tcagtacaat atcgcaacaa taatatatta aaaaaaatta tattattcaa
    16801 ctttatggtg aggattacac aatgagcagt ccaaatattc tcttaacccg tattgataac
    16861 cgtctggttc acggtcaggt tggcgtgacc tggacatcca ccatcggtgc gaatctgctg
    16921 gtagtcgtgg atgatgttgt cgctaacgat gatattcaac agaaattaat gggtattacc
    16981 gcagaaacct acggctttgg cattcgtttc tttactatcg aaaaaaccat taacgtcatc
    17041 ggcaaagctg caccgcatca gaagatcttc ctgatttgcc gtacgccaca aacggtacgt
    17101 aaattggtag aaggtggcat tgacctgaaa gatatcaacg tcggcaatat gcatttctcg
    17161 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
    17221 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
    17281 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
    17341 atgaaataac cctacttcag ggattatccc tggcggcgtt agttttcgtt ctggggattg
    17401 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
    17461 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
    17521 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
    17581 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
    17641 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
    17701 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
    17761 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
    17821 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
    17881 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
    17941 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccagg
    18001 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
    18061 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
    18121 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
    18181 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
    18241 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
    18301 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
    18361 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
    18421 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
    18481 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
    18541 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
    18601 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
    18661 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
    18721 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
    18781 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
    18841 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
    18901 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
    18961 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
    19021 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
    19081 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
    19141 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
    19201 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggctg
    19261 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
    19321 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctagg attggggaaa
    19381 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
    19441 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
    19501 cgtgggctca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
    19561 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
    19621 atcccggctt cgtttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
    19681 gatgaaaatt tgctcaaaat ttttaaatta acttattcaa
//
LOCUS       DAZUFB010000090        18666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZUFB010000090 DAZUFB010000000
VERSION     DAZUFB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.090"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHY3M_003585"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHY3M_003585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFS4318421.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHY3M_003586"
     CDS             707..1135
                     /locus_tag="ACHY3M_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFS4318422.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHY3M_003587"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHY3M_003587"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFS4318423.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHY3M_003588"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHY3M_003588"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4318424.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHY3M_003589"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHY3M_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFS4318425.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHY3M_003590"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHY3M_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFS4318426.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHY3M_003591"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHY3M_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFS4318427.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHY3M_003592"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHY3M_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFS4318428.1"
                     /translation="MSYEVLQLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHY3M_003593"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHY3M_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFS4318429.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHY3M_003594"
     CDS             complement(8154..9338)
                     /locus_tag="ACHY3M_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318430.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHY3M_003595"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHY3M_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFS4318431.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHY3M_003596"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHY3M_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFS4318432.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIDAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHY3M_003597"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHY3M_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFS4318433.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHY3M_003598"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHY3M_003598"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4318434.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTNELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHY3M_003599"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHY3M_003599"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4318435.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELASCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHY3M_003600"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHY3M_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4318436.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHY3M_003601"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHY3M_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4318437.1"
                     /translation="MRITIYTHNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHY3M_003602"
     CDS             complement(17180..17509)
                     /locus_tag="ACHY3M_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFS4318438.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /locus_tag="ACHY3M_003603"
     CDS             17661..18005
                     /locus_tag="ACHY3M_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFS4318439.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHY3M_003604"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHY3M_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFS4318440.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4675 a         4732 c         4827 g         4432 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa caccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgatag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agctgcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgtcgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 ttggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcatcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttggcatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 gccagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttat gagtgtaaat
    16921 agtaatgcgc atgattcgta tttccattta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aattttttat ttagtcagtt tttgtagata aaatattccg taacggctcg
    18661 tttttt
//
LOCUS       DAZUFB010000091        18651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZUFB010000091 DAZUFB010000000
VERSION     DAZUFB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.091"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..940
                     /locus_tag="ACHY3M_003605"
     CDS             <1..940
                     /locus_tag="ACHY3M_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFS4318441.1"
                     /translation="FLFSLPLIIFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHPR
                     SADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAR
                     YDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFR
                     SDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVG
                     RMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAF
                     YRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1043..1546
                     /locus_tag="ACHY3M_003606"
     CDS             1043..1546
                     /locus_tag="ACHY3M_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFS4318442.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1613..3070)
                     /gene="nuoN"
                     /locus_tag="ACHY3M_003607"
     CDS             complement(1613..3070)
                     /gene="nuoN"
                     /locus_tag="ACHY3M_003607"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFS4318443.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3077..4606)
                     /gene="nuoM"
                     /locus_tag="ACHY3M_003608"
     CDS             complement(3077..4606)
                     /gene="nuoM"
                     /locus_tag="ACHY3M_003608"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFS4318444.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4770..6611)
                     /gene="nuoL"
                     /locus_tag="ACHY3M_003609"
     CDS             complement(4770..6611)
                     /gene="nuoL"
                     /locus_tag="ACHY3M_003609"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFS4318445.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6608..6910)
                     /gene="nuoK"
                     /locus_tag="ACHY3M_003610"
     CDS             complement(6608..6910)
                     /gene="nuoK"
                     /locus_tag="ACHY3M_003610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFS4318446.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6907..7461)
                     /gene="nuoJ"
                     /locus_tag="ACHY3M_003611"
     CDS             complement(6907..7461)
                     /gene="nuoJ"
                     /locus_tag="ACHY3M_003611"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFS4318447.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7473..8015)
                     /gene="nuoI"
                     /locus_tag="ACHY3M_003612"
     CDS             complement(7473..8015)
                     /gene="nuoI"
                     /locus_tag="ACHY3M_003612"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFS4318448.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8030..9007)
                     /gene="nuoH"
                     /locus_tag="ACHY3M_003613"
     CDS             complement(8030..9007)
                     /gene="nuoH"
                     /locus_tag="ACHY3M_003613"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFS4318449.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9004..11730)
                     /gene="nuoG"
                     /locus_tag="ACHY3M_003614"
     CDS             complement(9004..11730)
                     /gene="nuoG"
                     /locus_tag="ACHY3M_003614"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFS4318450.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGIYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11783..13120)
                     /gene="nuoF"
                     /locus_tag="ACHY3M_003615"
     CDS             complement(11783..13120)
                     /gene="nuoF"
                     /locus_tag="ACHY3M_003615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFS4318451.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13117..13617)
                     /gene="nuoE"
                     /locus_tag="ACHY3M_003616"
     CDS             complement(13117..13617)
                     /gene="nuoE"
                     /locus_tag="ACHY3M_003616"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFS4318452.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13620..15422)
                     /gene="nuoC"
                     /locus_tag="ACHY3M_003617"
     CDS             complement(13620..15422)
                     /gene="nuoC"
                     /locus_tag="ACHY3M_003617"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFS4318453.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15516..16178)
                     /gene="nuoB"
                     /locus_tag="ACHY3M_003618"
     CDS             complement(15516..16178)
                     /gene="nuoB"
                     /locus_tag="ACHY3M_003618"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFS4318454.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16194..16637)
                     /gene="nuoA"
                     /locus_tag="ACHY3M_003619"
     CDS             complement(16194..16637)
                     /gene="nuoA"
                     /locus_tag="ACHY3M_003619"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFS4318455.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSARFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17268..18206)
                     /gene="lrhA"
                     /locus_tag="ACHY3M_003620"
     CDS             complement(17268..18206)
                     /gene="lrhA"
                     /locus_tag="ACHY3M_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFS4318456.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4659 a         5171 c         4808 g         4013 t
ORIGIN      
        1 atttctgttt tcactccctc ttattatttt ttaccaaccc tgggtcaatg cattaccgcc
       61 aatgccccga cacgcaaatc ctgagcaatt agaaaaaaca gtgcgttatc ttacacaaac
      121 tgtgcatcca cgtagtgccg acaatattga taatctgaat aggtccgcag agtatataaa
      181 agaggtcttc gtcagtagcg gtgccagaat tacctcgcag gacgtcccca ttacgggtgg
      241 cccctacaaa aacattgttg ctgattatgg tcctgccgat ggaccgctga ttattattgg
      301 tgcgcgctat gactctgcca gcagttatga aaacgatgaa ttgacctata cacctggcgc
      361 ggatgataac gccagcggcg tggcaggatt actcgaactg gcacgtttgt tacatcagca
      421 agtaccgaaa acaggcgtgc agctggtcgc ctatgcgtcg gaagaaccgc ccttctttcg
      481 tagcgatgaa atggggagcg cggtacatgc agcttcgctt gagcgtccag taaaattaat
      541 gatagcactg gagatgattg gctattacga ctctgcgcct ggtagccagg attaccctta
      601 cccggcaatg tcctggcttt atcccgatcg gggagacttt attgccgtgg tcggcagaat
      661 gcaggatatc aacgccgttc gtcaggtaaa agcgacattg ttatcatccc ggtatttatc
      721 tgtttattct atgaatgccc cagggtttat tcccggtatt gatttctctg atcaccttaa
      781 ttattggcaa cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa
      841 acaataccac ttgcccggtg ataccgcaga cagattgaat tatcagaaaa tggctcaggt
      901 ggtggatggt gttataaatt tgttatacaa cagtaaataa taaaaatttc tcgaggcggg
      961 tccagaaaaa gcgacatgca cattttattt tccctcgtct gttttaagat gcgaaacatg
     1021 aaattcaatg gaggaaaaat aaatgggaat gattggttat tttgcagaaa ttgactcaga
     1081 aaagattaac caactgcttg agtctactga aaaacctttg atggataata tacatgacac
     1141 gctttcagga ttacgccgac tggatattga taaacgctgg gattttttac actttgggct
     1201 gacaggcacc tctgcttttg atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg
     1261 tgaacacagc ctcgaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt
     1321 agccgcgaca atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc
     1381 cattaagcga ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga
     1441 ggggcaactc tttgcttcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg
     1501 tatgctgcga caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac
     1561 caaaaaaaac cgccgaacat gtcggcggct ttctgactta caaactaaca gattacatca
     1621 gcggcattgc taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta
     1681 ccaacagtgc agaaatcagc accacgatac cgcccgcgct gtactgccag tttgatggtg
     1741 catcgcgacc cggttgttcc ggggcatgaa gatacaggct caccgccacg cgcaggtagt
     1801 agtagaggcc gattgccgaa ccgacaacca cggcacccac cagccaccac aagtgtgcct
     1861 ggacaccgac cgccagcacg tagaacttac cgataaagcc cagcgtcatc gggataccgg
     1921 ccagagacag catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac
     1981 cgcggtaaga gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga
     2041 ccacgccgaa cgcgccgagg ctgctgaaca gataaccggc caggtaaacc cctaccgctt
     2101 ccatcgacat ctcaccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag
     2161 agatagatga gtaaccgagc agacgtttga tgttggtctg gctcagcgcc atcaggttac
     2221 cgaagatgat ggaggcaaag gcgataatcg ccagtactac gcgaatcgct tcgctgtcac
     2281 ccaccggtgc gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg
     2341 tcgccaggaa agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt
     2401 ggaacggcac cagcgagagt ttgaagccga ggccaacaat catcagtccg aaacctgcca
     2461 gcaacagcgg ctcgttgagc ataccgtcgc cgaggttttt acccagcgcg acaaacgaca
     2521 ggtcgccaga ctgtgcatac accagcgcca taccaaacag caggaaagaa gacgctgcgg
     2581 cagaaaggat ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac
     2641 cgaccaggcc aaacagcggc aaagagatca gttcaatacc gaggaacaaa gacgccagat
     2701 ggttggcatt cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact
     2761 catccttgtt gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc
     2821 tcgccaacaa taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg
     2881 taacgtccat agcgcccgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc
     2941 caataaccga gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca
     3001 caaccaccac cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag
     3061 ttattgtcat ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga
     3121 tattgccaat cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta
     3181 ccagcagcac caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt
     3241 cctggctggc aatctggctt ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg
     3301 aataaacaga tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct
     3361 ggaagctgcc gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa
     3421 gcgttgccac cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac
     3481 cgcccatcat gcgcatgtcg cgggtatgga tacgttcata aagctgacca caaagaataa
     3541 acagacccgc cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca
     3601 actggctgcc ggtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg
     3661 cgatcagacg tttgatatcg gtctgggcga aggccatcca cgcaccgtag aagatgccga
     3721 taacacccag ccacatagca attggcgcga actctgccga cgcgttcggg aacagcggca
     3781 gggagaaacg cagcaaaccg taagcggcag ttttcagcaa gatccccgcg aggtcaacgg
     3841 aaccggcggt cggagcctgg gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg
     3901 gcattttgac tgcgaaggcg atgaagaagc ccagcattaa caggtattcc acaccactgg
     3961 acattggcgt attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt
     4021 gaacaaaaac cagcgccagg atggcaatca acatcaccag gccactcgcc tgggtgtaaa
     4081 tgaagaactt ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca
     4141 gtgcgatcag gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca
     4201 tgtcgatggc aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga
     4261 agaagccctg atatttttcg atctctttcc acgaacatag taccgccagc acaccgagca
     4321 gaccggtcag cacgaccatc agcagcgaca gcccgtcaat ggcgagatga atagagatac
     4381 caaaacgcgg gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt
     4441 gcgtcagtga ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca
     4501 tggtgatcag cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca
     4561 ggaagccgcc aataaaggga attaatatta gccagggtag taacatggcg atctttattc
     4621 cttgtaaaag tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt
     4681 cggactgggg gtagtgtcgg atgcaccgcg aaggcggctt atccgaccta cgttcttata
     4741 tgcccagggg caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc
     4801 acgaccgcac cgatgctcat ggatgccaca taccagcgca gatagccgtt ctcacttaac
     4861 agcagacctt tacctgcaaa gcgggaaagg acagccggga tgttcatcat tgagttcagc
     4921 ggatcgcgtt tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcatac
     4981 agccagtcaa atccccaggc gttgtaccac caggtgccca gcagacggcc cggcgcactg
     5041 ttggcgatgg aggtcaccag agtacgttta cccagccaca gccaggctgc cagcagaatg
     5101 ccgaccaccg cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt
     5161 tccgtcgttt gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg
     5221 gaaaggatca gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg
     5281 tgaatttgtt cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc
     5341 ataaacgcac cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc
     5401 gcgaggatct catccttact gaagaagccc gcagtgacca gcggtagtgc cgacagtgct
     5461 gcgccgccca ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc
     5521 ttgaagatgt tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc
     5581 agcgctttaa agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg
     5641 ccaagcgcga ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg
     5701 atgtcggtct gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata
     5761 cccaccagat gcagaacttc cggcgtcacc aggaacaggc cgtgggtacg ggcgatcagg
     5821 tagacacccg cggttaccat cgttgcggcg tggatcagcg cggagacagg cgtcgggccc
     5881 gccatcgcgt cagcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg
     5941 cccagcagca tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt
     6001 gccagttcca ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt
     6061 gcgaaagcga ggaaaacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg
     6121 ccattcttcg gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct
     6181 tcccagccga ggtacatcag cagcaggtta tcggcgagaa ccagaactac catgctggcg
     6241 atgaacaggt tggtgtaggc gaagaagcga gagtagccct cttcaccgcg catgtaccag
     6301 gaggcgtaca tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac
     6361 aggccgtcca gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac
     6421 agcggctggc tgtatgactg ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt
     6481 accagcgccg ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag
     6541 cgcccacggg agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca
     6601 agcatgttca tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt
     6661 gaagttgcag cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga
     6721 gaatgtacat cacctgaccg tcggtctgac cccagtagct tccggcgacc acaaaggcca
     6781 gcgcggaggc gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga
     6841 taaccagacc ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta
     6901 aggggatcat gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag
     6961 cacttcaccc gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag
     7021 cagcatagaa gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc
     7081 tttagcactg attggcgtac cgtcgatacc ctgatcatta acaccaagga tggcgtaaac
     7141 aatcaccacc agcatgatgg ccgacaaaat tgccggacca atccacacct gcggtttcag
     7201 ccactggcgt tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac
     7261 gaacagcacc ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc
     7321 gcccagtgag aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc
     7381 gtgtaccgga ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca
     7441 gatataaaaa gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata
     7501 ggcttggctt cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc
     7561 cggtagaagt tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttttcg
     7621 taaaccagat cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc
     7681 gtggtcggac aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg
     7741 aaaaattccg ggtaccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg
     7801 accgggcagg ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg
     7861 cgggtcagaa cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac
     7921 attcgcgttt cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg
     7981 gtgccgaaac ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct
     8041 gccagagaat gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt
     8101 tccagccgaa ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa
     8161 tgaacatcat cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg
     8221 ggccttgcca gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga
     8281 tgtactcacc cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat
     8341 ccgccagttc ctgctcggct tccggctggt caaacgggtg acggtgacat accgccacgc
     8401 ccgcgatggc aaaggtaata aaaccaaaga attgcgggat aacgttccac acatgcgcct
     8461 ggctgttgac gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg
     8521 aaagcccgag gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca
     8581 acgagtattt gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg
     8641 ccatcatcag gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga
     8701 ctggcacaat cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa
     8761 agatgacgcg atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt
     8821 ccgcaaccag ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga
     8881 acagacccag caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca
     8941 gcaggatcac caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc
     9001 aactcattgt tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat
     9061 gccggacata cccatcggca aacccacctg ccctgccgtc agcccttcag cgatttcaac
     9121 cggcagcgtg accgtgttgc catcgtaact aaaggagacg cgagcgcctg cgttcacgcc
     9181 caacttcgcg gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa
     9241 gaccggagca cgctgtgaca attcatcgct gccaaacagg tggtaatacg gcgcgatacg
     9301 ccatttcccg tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt
     9361 ttcgctggtt tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc
     9421 gtcctggaat ttgttccacg cctgcgggga gttccagccc ggcgcccagg caaacggcac
     9481 ttgcgaacgg tgcgcagtcg gctggttgtt accttccatc gagaaggtga acatggtgtc
     9541 aatatcctgc ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc
     9601 gctgtaacgg tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg
     9661 cgcagcatct ttgatacctg ccagttccgg gattttcgcc acaacagcgt caatcacatg
     9721 gtcgagctgc gtccagtcca cttcacggct cagcagggtg ctgtgcagcg agtgtaacca
     9781 gcgccagctt tccagcatga cagttttgct gtcgtaataa gcaggatcgt aaacctggaa
     9841 gaaacgttgg gcgcggcctt cgttgttgat caccgtaccg tcgctttcag caaagctggc
     9901 agcagaaagt accagatggg cgttttccat aatcgctgtg cgttgatgat caaccaccat
     9961 caccagcggc gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag
    10021 atcgttttcc agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg tcaacgcttc
    10081 ttcaagcgaa ccgccaccca taatgcccag ccccatgctg ttgacggaac gggcaatcat
    10141 ggtgatacca acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg
    10201 aatcacctct atgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc
    10261 cagtgcctgc acgatgacgt cgattttgct ttgcagctca ggttcgatac cgtcaaccgc
    10321 tggcgcagag ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac
    10381 cggtgcgcgg taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac
    10441 aaacagcgga tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc
    10501 cactttctgt gctgccgcca tttcgcgcgc tttacctttc acagcctgac gcactgccag
    10561 cgcgacgcgc gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga
    10621 ttcgatttcg cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag
    10681 ttgcagacgt tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag
    10741 ttcacgcagc gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt
    10801 cttcgactga cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat
    10861 gaaatcatcg ccacgacgct gtactggctg acgcggacga tccttcaggt tgacgtaacc
    10921 gtaaccgaaa cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt
    10981 ttcgatacga cgcagttcgc cgtaacgttc accggggctg atgttacagc cgatggaaca
    11041 ttgctggcag atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg
    11101 cgttttgtcg gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct
    11161 ttccagcgtg ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaatgcc
    11221 cagatctgta ccgtcagcgt aatctttgta gtaacgcaca cagcggtagc aggcgatgca
    11281 gcggttcatt tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt
    11341 gaaacggtag cgacggaagc tgtgtccggt catcacggtc atatcctgaa gatggcagtt
    11401 accgccttct tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac
    11461 actttcacgg aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc
    11521 cggtgtcata caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt
    11581 caccgcacac tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg
    11641 aatatcaagg cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta
    11701 ttctttgccg tctacatgaa ttgtagccat tagcatgctt ccagttttct cagtcagaga
    11761 ctgagcgtta atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat
    11821 taatcaaatg ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat
    11881 atttgatggc gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac
    11941 ccgggcctaa gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcac
    12001 cacgctccag cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac
    12061 accagccgca ggattcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt
    12121 tgatctcatg gtcaaccgcc atcgccagcg ccgtacccag acggcttccc gctttaccga
    12181 tactttcgaa ttccatcggc aggtcaagat gcgcttcggt caggaagtcg gtgcccgcgc
    12241 cgcctggctg ccaggctttg aatttcagac cgtcacgcat accaccggcg taatcttcga
    12301 ggatctcgcg cgcggtagta ccaaacggca gttcccacag gcccggattt ttcacccgac
    12361 cggagaagcc catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact
    12421 ccacgccgtt agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg
    12481 tcggtttacc ccatgcgccg gaggtcgccg ggaatggagg cttcgagcgt gggttagcac
    12541 gacgtccttc cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc
    12601 cggtatggac gaacagttcg aagtcaaaac ctgttcccat aatatttttg ccaagcagac
    12661 ctgcttcggt ggcttcggca atggcacggc gtagattcac tgccgcttcg atatattcgc
    12721 cacgcaggaa gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt
    12781 ccaccagcag gtgcggcagt tgctccatca acaggcggtc tttataggtg cccggctcca
    12841 tttcatcggc gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc
    12901 tccacttcag gccagtcgaa aagcccgcgc cgccgcgccc tttcagacca gcgtctttta
    12961 cctgattaac gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac
    13021 catttttgct gcggtattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg
    13081 tcagcggatg cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc
    13141 agggatcgct tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tatttggccc
    13201 tttatcacag ttccccaggc agcaggttgg cagcagcgta aagcgaccgt caaacgtcgt
    13261 ttgccctggt ttgatgttca gttttttctc cagcgccgcc tgaataccct ggtaaccgtt
    13321 gatgtgacag accacgctgt cgcaataacg gatcacatgg cgaccaaccg gttggcggaa
    13381 gatctggcta tagaacgttg ccacgccttc gacatcgctc gccggaatac ccagcacatc
    13441 ggcgatcgcg tggatcgcgc cgtccggcac ccagccacgc tgcttctgaa cgattttcag
    13501 cgcttcaatg gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc
    13561 acgctctgcc gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat
    13621 tagcggtcca catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac
    13681 accaggctgc cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg
    13741 atacgagtgc gataactcat ggtgctaccg tcactggtca ggtagtaact gttgatcccc
    13801 ttggtcgcct caatcatctg gaaagattca ttggcaggca tcaccggccc ccacgacact
    13861 tgcaggaagt gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc
    13921 gtggtcagcg ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca
    13981 agaatgcgca gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca
    14041 gaaacaccac caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc
    14101 gccttacgca cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac
    14161 tccagcgcct ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta
    14221 ttttgcagcg ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca
    14281 cgcagcaggc gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac
    14341 gccgggtgca tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg
    14401 gtaaaggcga agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac
    14461 agcaggtggc tgttgatacg gaacagttcg gagagcataa cgcgaatgac gttaacgcga
    14521 tccggcacgg tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg
    14581 cagccgccga ggtattcaat acggtcggta tacggaatgt agctgtgcca ggactggcgc
    14641 tcgcccattt tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc
    14701 tcttcgccgt cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg gtggttcgga
    14761 ccgaggttga ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc
    14821 ggtttgaagg tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc
    14881 gagaattcgg tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc
    14941 ggcatcatga tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag
    15001 gtttcacgct cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg
    15061 tcgttttctg ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga
    15121 tggtagaaaa cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg
    15181 tccatgccgt gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg
    15241 ccaacttcca gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc
    15301 tgaacagtaa aggcatccgg cccaaaacgg ttgcgcagtt cgccaattac cggatcatca
    15361 agatgatcgc gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc
    15421 attgcaaatg gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat
    15481 aagcgaagaa aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg
    15541 ttggttacgg caatacgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga
    15601 taaacgccct gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc
    15661 tgcaacagca tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac
    15721 acatcaaccg ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacatacca
    15781 ccagagttgg cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac
    15841 agacgctgaa taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca
    15901 gcctgacgcg gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg
    15961 gtaaacgaag tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt
    16021 gagtttttac gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta
    16081 acttcttgct ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc
    16141 tcaccgttgg gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt
    16201 tgacgattag cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc
    16261 gtccagtcca gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata
    16321 aaaattgcag cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg
    16381 aacagataca gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacctg
    16441 gcggacaggc gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt
    16501 ttcgacctcg cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac
    16561 aggccaatgg caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg
    16621 gatgttgaca tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg
    16681 gcagatggac gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag
    16741 aaattctttt atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa
    16801 ctttaacaaa agtttcttca cattagttta cataatatca acaccattag catttaatgc
    16861 cctttcaccc cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt
    16921 tgtgaagcaa tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc
    16981 ccaaaagaga gtattttctt gatctgtgac acgcttttgt cattccataa caaaaacgca
    17041 gcaacaaatt tacgtatttt ttaacatcat tgtagcaggt gatttttttc aggcgattat
    17101 ttgtgcgttc gggacgtgaa tctctggtga ttgaaaaatg aacagttttg tacgttctgc
    17161 actatgcgag gaaggatttt tactaaaaaa aagccgctgg ggtttaaaac acccccagcg
    17221 gctcgttttt tacactattg tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc
    17281 ctttcaatca acaatgaatc atccccttcc ggagcagaca ttgggctgta ttgccacgga
    17341 ttatggtagc tctccatcgc ctgatagatc acctgggcca gctcattatt actggacggg
    17401 tcatagcaca gcaaatattc ggtatcaggt agcggcggta agccatctac tccactcagc
    17461 acacgcaggt ccgggctcat catctcaacc ggccttgccg tcacgccaag accggctttc
    17521 actgccgcac gaacggccgg aagcgtcgag gcgacataag ccagtcgcca tggaatatct
    17581 gctttattaa gcgtcgccag caccatatcg cgaaacggac ttggatcatc cagcaataca
    17641 agagggatcg gctcgccttt ttgcaatacg tattccgctg cgcagtacca gtgtgttggc
    17701 gaggttcgca ggttcaatgc cttaaacgct gaaggacgat gggtggttac catcaaatcc
    17761 acttcctggg attccagcat ttctgccata taggcattac gtttaacgcg gacatccagc
    17821 gctaatttcg gataaaccga actcacgcga tttaacagga aaggtaagat cgtatcggca
    17881 gattcatctg aagcaccgat agttaacacg ccctgaagat tactgaacat taatgatgag
    17941 caggcctcat cattaaaacg caggattttc ctggcgtaac caagaagttg aatgccatgt
    18001 tcagttaaca gtttgttgcg accgtgacga gcgaacagtt ctttcccaac gagttgttcc
    18061 agacgctgca tttgctgact tacggcggac tgagtacgac acacagcggc agctgcggca
    18121 gcaaaagtgt tcagatcggc aacagcaaca aatgttctca gcagatcgag gtcgaggtta
    18181 attatcggac gatttgcact tatcatatat tatcacttac tggcggctca tactgagctg
    18241 gttaatgctg tgcacacaca aacaagcaat tccatttgta atgtgcctcc ctggcagttt
    18301 catccggaac ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca
    18361 ttgttccgtt ataatgcctg aagtagatca cagaatatat cttcagggat cgcatatcta
    18421 ttaagttact cactcttttc tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga
    18481 gtccttgttc cactctcgtg cagcatcgct ggtcatacgc gaacacgtac aaacagcaat
    18541 ggtgaggcgg catcaagagc agggatccgt catttatgcg agcattttac cccaaaaacc
    18601 ttttatttat aagggtcatt gcgaattatc tgatgcaaag ttatgttatg t
//
LOCUS       DAZUFB010000092        18556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZUFB010000092 DAZUFB010000000
VERSION     DAZUFB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.092"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..262)
                     /locus_tag="ACHY3M_003621"
     CDS             complement(20..262)
                     /locus_tag="ACHY3M_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFS4318457.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(274..552)
                     /locus_tag="ACHY3M_003622"
     CDS             complement(274..552)
                     /locus_tag="ACHY3M_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318458.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            complement(563..808)
                     /locus_tag="ACHY3M_003623"
     CDS             complement(563..808)
                     /locus_tag="ACHY3M_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFS4318459.1"
                     /translation="MLCPDFISWPWYFHHKSISTWLIEKAGIVVHRCQRNPFTGNRIF
                     ALSNGDNQFGQDFTLYEALRTVDRLLRGQSFIKQTDL"
     gene            750..1541
                     /locus_tag="ACHY3M_003624"
                     /pseudo
     CDS             750..1541
                     /locus_tag="ACHY3M_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1552..1947
                     /locus_tag="ACHY3M_003625"
     CDS             1552..1947
                     /locus_tag="ACHY3M_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318460.1"
                     /translation="MEKTVNSDLHLGQYWRIDIVCDPELRDEVGQYFSLHDVGFSKIE
                     VFSVENPYKLALFFDFAKKGVEVAKAIMGLLDRNDIEITMYRATDSSPQSVKSIKLRK
                     SEDVEKCKDLLSTCVAIGVQRNKEKEGSE"
     gene            2137..3117
                     /gene="btuC"
                     /locus_tag="ACHY3M_003626"
     CDS             2137..3117
                     /gene="btuC"
                     /locus_tag="ACHY3M_003626"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFS4318461.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            3180..3731
                     /gene="btuE"
                     /locus_tag="ACHY3M_003627"
     CDS             3180..3731
                     /gene="btuE"
                     /locus_tag="ACHY3M_003627"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFS4318462.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            3731..4480
                     /gene="btuD"
                     /locus_tag="ACHY3M_003628"
     CDS             3731..4480
                     /gene="btuD"
                     /locus_tag="ACHY3M_003628"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFS4318463.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            4558..5022
                     /locus_tag="ACHY3M_003629"
     CDS             4558..5022
                     /locus_tag="ACHY3M_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4318464.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            5268..5981
                     /gene="ydiV"
                     /locus_tag="ACHY3M_003630"
     CDS             5268..5981
                     /gene="ydiV"
                     /locus_tag="ACHY3M_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFS4318465.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            6044..7480
                     /gene="selO"
                     /locus_tag="ACHY3M_003631"
     CDS             6044..7480
                     /gene="selO"
                     /locus_tag="ACHY3M_003631"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFS4318466.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(7484..7675)
                     /gene="hemP"
                     /locus_tag="ACHY3M_003632"
     CDS             complement(7484..7675)
                     /gene="hemP"
                     /locus_tag="ACHY3M_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFS4318467.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(7807..8853)
                     /gene="aroH"
                     /locus_tag="ACHY3M_003633"
     CDS             complement(7807..8853)
                     /gene="aroH"
                     /locus_tag="ACHY3M_003633"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFS4318468.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(9010..9843)
                     /gene="ppsR"
                     /locus_tag="ACHY3M_003634"
     CDS             complement(9010..9843)
                     /gene="ppsR"
                     /locus_tag="ACHY3M_003634"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFS4318469.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            10176..12554
                     /gene="ppsA"
                     /locus_tag="ACHY3M_003635"
     CDS             10176..12554
                     /gene="ppsA"
                     /locus_tag="ACHY3M_003635"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFS4318470.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(12611..14257)
                     /gene="fadK"
                     /locus_tag="ACHY3M_003636"
     CDS             complement(12611..14257)
                     /gene="fadK"
                     /locus_tag="ACHY3M_003636"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFS4318471.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGIEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(14314..14607)
                     /locus_tag="ACHY3M_003637"
     CDS             complement(14314..14607)
                     /locus_tag="ACHY3M_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS4318472.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(14604..15893)
                     /locus_tag="ACHY3M_003638"
     CDS             complement(14604..15893)
                     /locus_tag="ACHY3M_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4318473.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGSKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(15949..16887)
                     /locus_tag="ACHY3M_003639"
     CDS             complement(15949..16887)
                     /locus_tag="ACHY3M_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFS4318474.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(16907..17671)
                     /locus_tag="ACHY3M_003640"
     CDS             complement(16907..17671)
                     /locus_tag="ACHY3M_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFS4318475.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDIEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            17987..>18556
                     /locus_tag="ACHY3M_003641"
     CDS             17987..>18556
                     /locus_tag="ACHY3M_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4318476.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLN"
BASE COUNT         4527 a         4493 c         4798 g         4738 t
ORIGIN      
        1 agccatgaga taaatatact catgcaatat tccccagctt atacggttca atatgctccc
       61 cgcattctgc ggcacagatc agctcggaaa gttcgttaag tgcatccaga tcatcagcgt
      121 aaaaagccac gtcatacaga ctccggattg ccctggtcaa tgagtcacgg gctgcacgtt
      181 cagcatgagc acctgatgca cttaagcgaa aataaaaacg ctcaagtgct ttgttaatga
      241 gagttttata ttctttgccc atcgcaacgt cctttaatct gctttctgaa tttcagcttc
      301 tgaatccata caaataattt cgatataggg ttcatcgcca ttaatcttac gtgctttttc
      361 agcttcgcta atgattccgt gtacagtctg gtacggaagt tctacggtca ggcgcgtgcc
      421 gttcagataa acgtaagtag ctgcattttt ttctgatggg acaactccat caatagctga
      481 tgcgcgcaat aacagttcac cgcgaaaatc aataaaacgg ataaatacac cttgtgcatg
      541 ctctttggtc ataacgcacc tgttataaat cagtctgttt aataaaactt tgcccgcgaa
      601 gcagacgatc aaccgtgcga agtgcttcgt acaatgtgaa atcctgtcca aactgattgt
      661 cgccgttgct cagagcaaaa atgcggtttc cggtaaatgg gttgcgttgg catctgtgaa
      721 ccacgattcc agctttttca atcagccagg tgcttattga cttgtggtgg aaataccacg
      781 gccagctaat gaagtcaggg cataacacgc gccttaaatt gctgcgcttg agtgaggcaa
      841 tggatgaccc gtgcgtgcat aaacttaata caacgatgct caccgagcta cgtgtgtcca
      901 ggatagagca ggggatacag cccagcacca taaatcgaga gattggggcg ttaagcgcga
      961 tgtttaccgc actcatctca tccggccatt ttcttaacga taaccccgtt caaggcctta
     1021 aaggaatgaa ggttaacgag cgtgaaatgg gatatctgag taagtctgaa tgtgttcagt
     1081 tgctggatgc actggctgaa aatcccgatg aacggctggc cgtcgaaatc cttctgtcga
     1141 ccggggcgcg atggggcgag gtagcggcac tggagcagcg ccgtgttctt cattgtcgaa
     1201 tcactttttc aaaaacgaag aacagcaaaa accgtaccgt tcctatttct gaaagcctgt
     1261 ttgaaaagat caaaaaacgg ggcgggaaac tggtgtttcc gacgctggat tattcattgg
     1321 ttcgcgatgt catcaaaacg gtcgcacctg atgttcctga cggccaggct gttcatgcgc
     1381 tgcgccacac cttcgccagt catttcatga tgaacggcgg caatattctg acgctccaga
     1441 aaattctggg gcacgcaaag attcagacaa cgatgattta tgcccatctt gcgccggatt
     1501 acttgcagga tgcggtgaga tttaatccta ttgctgggta aggaggatgt tgtggaaaaa
     1561 acagttaata gcgatttgca tttaggtcag tactggagaa tagacattgt atgcgatcca
     1621 gaacttcgag atgaggttgg gcaatatttt tctctacacg atgttggttt tagcaaaatt
     1681 gaagtttttt ccgtcgaaaa tccatataaa cttgctctat tctttgattt cgcgaagaag
     1741 ggtgtggagg ttgcaaaagc cattatggga ctcttagacc gaaatgatat tgaaataacg
     1801 atgtatcgtg ctacggacag tagcccgcag tctgtaaaaa gcatcaaatt gcgtaagtct
     1861 gaagacgtag agaaatgtaa agatttgctt agtacgtgtg ttgcaattgg cgttcaacga
     1921 aataaggaaa aagaggggag tgaatgatcc acaaagtgac cacatccctg ttatttgttg
     1981 tggttggctg tgtttttgtg tgtctgtaag tctttgataa ttatctaact tattgatttt
     2041 tgtttgtgtt tatggccgct ctgcggcctt ttttcttttc actgtcgaag agtcaccgta
     2101 aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa
     2161 cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc
     2221 ttaagccttt gcgccggtga acaatggatc tcgccaggtg actggtttac tcctcgtggc
     2281 gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg
     2341 gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct
     2401 ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt attgcttggg
     2461 caagggcaac tccctaactg ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc
     2521 actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct
     2581 ggcgttgcat tagggattat ctgtagcgca ctaatgacgt gggctatcta cttttccacc
     2641 tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg
     2701 cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc
     2761 aggccgatga atatgttagc acttggcgag atctcggcgc ggcaactggg tttacccctg
     2821 tggttctggc gcaatgtgct ggtggccgcg accggctgga tggttggcgt cagtgtggcg
     2881 ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg gttgtgtggt
     2941 ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg
     3001 ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc
     3061 accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcaag acgttagccg
     3121 caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccca ggagaaacga
     3181 tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg
     3241 agaagtacgc tggtaatgtg ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc
     3301 cgcaatatga gcagttggag aatattcaga aagcctgggc cgatcgaggt tttgtggtgc
     3361 tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa gagattaaaa
     3421 cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg
     3481 gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc
     3541 cggaagagag cggattctat gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg
     3601 atgatatttt atggaatttt gagaaatttt tagttggcag ggacgggttg gtcatccagc
     3661 gtttttcccc ggatatgacg cctgaagatc ccattgtgat ggaaagcatt aaactggcgt
     3721 tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaaacta cccgcctggg
     3781 gccgctttct ggcgaggttc gggctgggga gatcctgcac ctggtggggc cgaatggcgc
     3841 gggtaagagt accttgcttg cacgaatggc cggaatgacc agcggtaagg gaagcattca
     3901 gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta
     3961 cctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca
     4021 tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga
     4081 tgacaaactc ggacgtagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct
     4141 tgctgcggtg gtgctgcaaa tcacaccaca agccaatccc gcaggtcaat tgctgcttct
     4201 tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag
     4261 cgctctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacatt
     4321 gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca gtggacgcag
     4381 agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct
     4441 ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt atcttgaaaa
     4501 tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg
     4561 cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca
     4621 ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag
     4681 ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac
     4741 tgttcgggat ttgtggttgt aacgatgcgc gatcgtttcg atttgcagct gccccgagaa
     4801 accaaagaac aagcctctat cggcacgcaa attgataaag acgagttgct gcctggtgac
     4861 ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat ttacgatacc
     4921 aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat
     4981 gtctattggc agaaaaattt ctggcaggcg agacgaattt agtatgtcga aaaatggacg
     5041 gcgatgatac atcgccgtcc atttactcct gaatacagta gtattttgca gacaaaaatt
     5101 ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaagacccc tgaaaaagtg
     5161 gttaacatgg aacaaatgaa agttactcct tatgtaattg ttatgtctta tggcctcatc
     5221 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
     5281 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaagacc
     5341 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggtacggtt cgtatcccga
     5401 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
     5461 tggaattact aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta
     5521 atttaacgcc acaagtggct acattattat tagagaggga taattacgca ggcgaactat
     5581 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
     5641 aagataatcg cgatctttta tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg
     5701 gagcaggtaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtcatgctgg
     5761 ataaaagttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
     5821 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgacag
     5881 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
     5941 ccgttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
     6001 ttaagacgag agtaaccgtc tacactatta aacaggagga tctatgaccc tgtcttttat
     6061 tacccgctgg cgcgatgaat tgccagcaac ctatacagca ctttccccta cgcctttaaa
     6121 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
     6181 gctgtttaaa aatggcgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
     6241 actggcgcag gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg
     6301 gcgcggtatt ttactcggcg aacaactgct tgctgatggc actacaatgg actggcatct
     6361 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
     6421 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
     6481 cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
     6541 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
     6601 tcgccgcgag ccggaaaagg ttcgtcagtt agctgacttt gccattcgtc attactggtc
     6661 atatcttgaa gatgatgagg acaaataccg cctctggttt agcgatgttg tcgcacgtac
     6721 cgcatcgtta attgcccaat ggcagacggt cggctttgct catggggtga tgaataccga
     6781 taacatgtcg ctgctggggc tgacgcttga ttacgggccg tttggttttc tggatgatta
     6841 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca
     6901 acctgctgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
     6961 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
     7021 acaacggatg cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact
     7081 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat
     7141 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg
     7201 tgcggcattt gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag
     7261 cgatagtgag cgtcagcagc tgatgcaaag cgttaaccca gctctggtgt tgcgcaactg
     7321 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct
     7381 gcatgaggcg ttgcgaaatc ctttcagcga cagagctgat gactatgtca gtcgtccacc
     7441 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca
     7501 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
     7561 tgccatccgg acctaacagt gtctggctgg aaattcgccg aataggctgc ggggaagcgg
     7621 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
     7681 ttaataataa ttgatataac aatgattctt attatcaata ttatggctga ccagagcaag
     7741 cagtaatgtg ctgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc
     7801 acgcattcag aagcgggtat ctaccgcaga ggcaagtttt tcgaccaggc gttcggtatc
     7861 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
     7921 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tcgccgtaga
     7981 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
     8041 ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag
     8101 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
     8161 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
     8221 tggcgagaga aacatatggc tggcgcgggc tgcgcggata gcatccacgg caatccgcgt
     8281 gttgccatcg gtaccatttt taaaacctac cggacaggag agtgccgaag ccatttcacg
     8341 gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc caactgatta aatcagcaat
     8401 aaactgaccg gtcaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac
     8461 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa
     8521 atccggatcg gagattagcc ctttccagcc gacaacagtt cgtggttttt caaaataggt
     8581 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
     8641 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
     8701 cttatcttca ccattcagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
     8761 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
     8821 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
     8881 acggcaggac taccgggaag tgatgcgcat cacgataaac caatcgcagc tgatttcaag
     8941 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaat aagctaatgc
     9001 actagttctc tagtacattc ggcggctaag ccccatgata tcgaggatct tggtggcaat
     9061 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaacgc
     9121 ttctacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcggc tgttctcccg
     9181 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
     9241 ctgaagcggt ttgagcgacg cgggtagcac cagattgtcc atatcgtcgg caataaaggg
     9301 gtagtttgcc gcgcggatac caaactgcat tgccagatac agactggtgg gggttttacc
     9361 acagcgagaa acaccgagca ggatcacctg agcctggtcc agattgcgca acgaaatgcc
     9421 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
     9481 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
     9541 ttgtagcggg gcaaccagcg cctgaacaat atcctggcaa aagccttcac tttgcaagat
     9601 gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cacctgtctg
     9661 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
     9721 cggcagcgta atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
     9781 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
     9841 cattttgttc ttcccgtgat gcagacataa ttctatgaaa gcataaatta aaaacgcaca
     9901 gaagcgtagg acgtaatgtc tggtttataa aatgaacctt caattttatt ttttatgaaa
     9961 acagcatttc atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaata
    10021 gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa
    10081 cgattcaccg tttttttcat ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt
    10141 ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc
    10201 tggtgctttg gtataaccaa ctcggcatga atgatgtaga cagggttggg ggcaaaaatg
    10261 cctccctggg tgaaatgatt actaaccttt ccggaatggg tgtttccgtt ccgaatggtt
    10321 tcgccacaac cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca
    10381 tttatgaact gctggataaa acggatattg acgatgttac ccagcttgcg aaagcgggcg
    10441 cgcaaatccg ccagtggatt atcgacactc ccttccagcc tgagctggaa aatgccatcc
    10501 gcgaagctta tgcacagctt tccgccgatg acgaaaacgc ctcttttgcg gtgcgctcct
    10561 ccgctactgc agaagatatg ccggacgctt cctttgccgg tcagcaggaa accttcctca
    10621 acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca tgtatttgct tctctgttta
    10681 acgatcgcgc catctcttat cgtgtgcacc agggttacga tcaccgtggc gtggcgctct
    10741 ccgccggtgt tcaacggatg gtgcgctctg acctcgcatc ttctggcgtg atgttctcca
    10801 ttgataccga atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg
    10861 aaatggtcgt gcagggcgcg gttaacccgg atgagtttta cgtccataaa ccgacacttg
    10921 cagcgaatcg cccggctatc gtgcgtcgca ccatggggtc gaaaaaaatc cgcatggttt
    10981 acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga agacgtaccg caggaacagc
    11041 gtgacatctt ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa
    11101 ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc gaaagatggc cacaccggca
    11161 aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc
    11221 gttatacgct gcattcacag ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca
    11281 tcggcgcggg tccggtgaaa gtcatccatg acatcagcga aatgaaccgc atcgaacctg
    11341 gcgacgtgct ggtcactgac atgaccgacc cggactggga accgatcatg aagaaagcat
    11401 ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac
    11461 tgggcattcc ggcggtagtg ggctgtggtg atgcaacaga acggatgaaa gacggtgaga
    11521 acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat
    11581 ttagcgtgaa aagctccagc gtagaaacga tgccggatct gccgttgaaa gtgatgatga
    11641 acgtcggtaa cccggaccgt gctttcgact tcgcctgcct gccgaacgaa ggcgtgggcc
    11701 ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg
    11761 agtttgacga tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg
    11821 attctccgcg tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg
    11881 cgttttatcc gaagcgcgtc attgtccgtc tctctgattt taaatcgaac gaatatgcca
    11941 acctggtcgg tggtgagcgt tacgagccag atgaagagaa cccgatgctc ggcttccgtg
    12001 gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt cgcgctggag tgtgaagcag
    12061 tgaaacgagt gcgcaacgac atggggctga ccaacgttga gatcatgatc ccgttcgtgc
    12121 gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc gcgtcagggg ctgaaacgtg
    12181 gcgagaacgg gctgaaaatc atcatgatgt gtgaaattcc gtccaacgcc ttgctggcag
    12241 agcagttcct cgaatatttc gacggcttct caattggctc aaacgatatg acacagctgg
    12301 cgctcggtct ggatcgtgac tccggcgtgg tgtctgaatt gttcgatgag cgcaacgatg
    12361 cggtgaaagc attgctgtcg atggctatcc gtgccgcgaa gaaacagggc aaatatgtcg
    12421 ggatttgcgg tcagggtccg tccgaccatg aagactttgc cgcatggttg atggaagagg
    12481 ggatcgatag cctgtctctg aatccggaca ccgtggtgca aacctggtta agcctggctg
    12541 aactgaagaa ataaaataaa tccccggcgg cgttcagtcg ccgggtttat gtgatccccg
    12601 aagataaaac ttattcaatc tcttcacaga catcctgcgt taatcgccgc ataatatctt
    12661 ttcttaacaa aaacttttgt attttacctg aggcagtgcg cggtagtttt tcgattacca
    12721 cgatatgttc aggatattta tattttgcga cccgtttacg gctaaaaaaa gccaccacct
    12781 cttccagcga taatgaatga tgcggcgctt tcagcacgac ataagcgcat gatcgttcac
    12841 ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg aattttagga tgctgcaata
    12901 aaatatcttc cacttcacgg ctgctaatat tttcgccgcc gcggacaata atatcttttt
    12961 tgcgtccggt aatttttata tagccagcct catccatacg gcagagatcg ccgctgtaat
    13021 accagccttc ttcatccagc gcacgggcgg ttaattcagg ttcatcaaaa tatcccataa
    13081 acacattggg gccacgcgag gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg
    13141 cgtcatcgac cactttaatc tctacacctg cggcagcgta accatcggtg tgcataaagc
    13201 gcgacaaagg atcatcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa
    13261 cacttaataa tttaatgccg agctgctggc attcacgcgc cacttttttg gggattgtgg
    13321 ttccgccgca aagaaagaaa cgcagcgctg acaggtccgc gggttgtttc tctagtacat
    13381 tcaaaagatc atagacaaac ggcgttgcgc ctagcataca ggtgcaacgc tgctgctcaa
    13441 gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta
    13501 agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg gccgagtggc gcaggcatca
    13561 taaagacatc ctgccaggtc agattcagcc gcgcgcaata agcccgctca ctggcgagaa
    13621 tattgttatg cgttagcatc acgccctttg gcagaccctc gattccggag gtaaacagca
    13681 ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt cagtggggta ttgtcggcga
    13741 taatctgact taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt
    13801 gttgtagttg tggaagctga ttttgcagcg gcaggattaa atctaccggg cgcgtttgtt
    13861 taaacaacgt cggtgcaaag aacatttttg cctgacactt attgagcacc cacaccagtt
    13921 ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc accgattttc aggcaggcaa
    13981 gatagataac ggtaaattca caccagccag gcagttgaaa tgcgatgcga tcgcctgatt
    14041 caataccctt cgctaacatc cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat
    14101 aggtgtacga tgcaccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg
    14161 tctgctgcca gtaatcggcc agcgaagcat cgccccatag cccttgctga cgatacgccg
    14221 cacgacgttg ttcgttgaac gttaatgtca ctttcatgtt ggactctcga aacagaacat
    14281 caggcccgag atgcgggcct gtgggatgca tgattagccg tagcgaaaat caataccgaa
    14341 ggtgcctgcg ggatattgcc actgttcgag aatagtgtta ccgcacagca cccgacaggt
    14401 gccgcactcc agacaaccgg cagaatcaaa atgaatgttg ccggcgtcat cctgcttata
    14461 aagtccggca gggcaggctt tcattaattt acggaattca ttgatatcgg gatttgccgc
    14521 cagaatgata tgcgggtggc cctcatcaac atggaattta ttgacgccta atttgatgtc
    14581 gacgttaacc gtagcgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc
    14641 aagttgatca gcccaatttt cttcgcatgt cccatgatca ttttgcgtac cggctggttt
    14701 ggtttgccgt caatggtgaa catctcgttc atgatgtcgg cgaccattcg tgggtattgg
    14761 ctaaacaggc gcgggttttc catcagcgcc gggatcttgc ggaaatgctg catatcgcgc
    14821 atgacgcagc tttgttccag ctcacgtttg tattgcgcca gactgctggc ggagaaatcc
    14881 tcgcgttctt tggcggcgat caccgttgtg gcggcagcct gagccgatgc aatggctaaa
    14941 tccatgccac gaactgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc
    15001 atcacgccat cgttaaccat ctgcggcacc attgccagac cgccttctgg caccatatgc
    15061 gcggaatatt caagcagttt gctgccgcta attagtgggc gaatggcggg gtgttgttta
    15121 aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa
    15181 accagcccca aggatatgga atccttgttg gtatagagaa atcccccgcc catcaggccg
    15241 tcagaagggg aaccggcaaa cagccaggcg gcaccttcct cgcccgtaat attaaagcga
    15301 tcgttgatct gttctggtgt gaggccaata acctctttaa caccaacagc gtaatgatgc
    15361 ggatcggaag cgggaaccat tcccagcgag cggccaagca tcgagttaac gccatcagcc
    15421 agaatcacca cattcgcttc gagaatatca tccccagcct gcacgccagt gaccttgttt
    15481 ccttcacgaa ccagcgcatc gacgcgaacg cctgggataa actgcgcgcc cgcctgctcg
    15541 gcttgttcca tcaaccacgg gtccagacga ttacgcaata cggtataaga tgcgtgttgc
    15601 ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg cgctttcttc ggttaagaag
    15661 gagattttct cgcgtgtgac cttgcgttct accggcgctg atactgcaaa gcctggaatg
    15721 attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg
    15781 tcgccgcgtt ctatcaccag gacatccagc ccggctcgcg ccatgaccag tgcggcaacg
    15841 ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt tgtcttccga catgcgttat
    15901 gctcccttcc cgctgcgttt acagcgggag atgtggttga aaggggaatt agcggctcaa
    15961 ctggctaatc agggcaggga cgactttgta gatatcgccc accagaccgt agtcggcata
    16021 attgaagatt ggcgcatttt tatctttatt gatggcgaca atcacttttg cgccgttgcc
    16081 gccaaccata tgctggatct gcccggagat ccccagcgtc aggtagagat cggatttcag
    16141 caacacgccg gagacaccga tataacgttc acgctccatc cagttctcgc cttcggcaat
    16201 tggacgtgaa cagccgactt cagcattcag caccgccgcc agttcgtgga ccattttcag
    16261 gtcatcctgc gccgccagac cacgaccgac gccaaccaca cgttttgctt tgctcaggtc
    16321 cacgctgctt gcggctttcg cacggcgttc gcgacagaga atttcatgac gcggagcaac
    16381 ataaggtacc gtttctgtcg ggcactgatg agaggtatca ctggtgcacg gttcctgaac
    16441 accgggtgca agggtaatga tcgccagcgg gctgttgatc ttttcctgag cgaacgctaa
    16501 cccgccatac atccggtgtt cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc
    16561 atcgttcacc agcgccgcat tcagttgcac acttaaccgt gctgccagag ctttaccacg
    16621 tttcgtcgcg gccaacagca acatagcggg gtgtttatct ttcagcaggg cagcaatgct
    16681 ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc tcaagaacat aaatacattt
    16741 tggaccataa ggcataactg cctgcgcctg gtcggtattt tgtacaatgg catacacctg
    16801 ttggccccat tgctgagcgc cgccaaacag ttcagcataa cgttcaggat tatcgctaaa
    16861 gacccagacg ctgtttaatt gactcatcgt ttcttctccg taggcttcag ttcagggctt
    16921 tcttcagatg ttccgccagc tcggcaatgg cctccggcga atcgttatcg ataatgatgt
    16981 gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag ttcagcaagt ggcgcgctct
    17041 ggctccagtc aatatcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa
    17101 tggctttcat cgaaggaatg cgtggcacgt taatatcgga ggtgacgcag agcacggctg
    17161 gaacagagag ttcaataact tcaatatcat cttcaagcgt gcgttcaatc accagtgtat
    17221 tgccctgacg ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccga
    17281 ccagcaagcc aacctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat
    17341 cgaagccgat cttttcaatt gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat
    17401 gttcaagttg cgcatcctgc accaaataca ggctgtgcgg cccgcgggat agcacgtctt
    17461 tgcgcacttt cgagttctgc aacaatgagc cgccaacggt cagcgcggct atctcatcgt
    17521 catctgttgc gagctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg
    17581 cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc tgttcttcag
    17641 gcaccagctt aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc
    17701 atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg cggggttttc
    17761 gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca
    17821 aaagtgcaat ttgcggcaat atagttattc catgaccgta caatcttgca ttcagtgtaa
    17881 ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta
    17941 tccggcatat attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg
    18001 ttttgataac gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt
    18061 tgcatttagc gatgatccaa aatgggagtc cggacatcac gttcatgaca atgaaaccga
    18121 gttgatttac gtcaagaaag gggttgcaag gtttaccatc gattcttcgt tatatgtcgc
    18181 acatgcggat gacattgtgg tgatagaacg cggcaggcta catgcggtgg cctctgacgt
    18241 taacgatccg gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga
    18301 ggaaaatcag ctactgcaac cgcattcttg tccggtaatt gccgcggggc agggaaaaga
    18361 agtcataaaa accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca
    18421 aacatcttcg ttatgggatg catttgccta tacgttagcc attctttact acgaaaattt
    18481 taaaaatgct tatcgttcgg agcaggggta tattaaaaaa gatgttctga taaaagatat
    18541 tcttttctat ctgaat
//
LOCUS       DAZUFB010000093        18556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZUFB010000093 DAZUFB010000000
VERSION     DAZUFB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.093"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHY3M_003642"
     CDS             complement(<1..209)
                     /locus_tag="ACHY3M_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4318477.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQ"
     gene            217..642
                     /gene="tnpA"
                     /locus_tag="ACHY3M_003643"
                     /pseudo
     CDS             217..642
                     /gene="tnpA"
                     /locus_tag="ACHY3M_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(706..999)
                     /locus_tag="ACHY3M_003644"
                     /pseudo
     CDS             complement(706..999)
                     /locus_tag="ACHY3M_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1271..2065
                     /gene="yjfZ"
                     /locus_tag="ACHY3M_003645"
     CDS             1271..2065
                     /gene="yjfZ"
                     /locus_tag="ACHY3M_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS4318478.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHTQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2136..2585)
                     /gene="rplI"
                     /locus_tag="ACHY3M_003646"
     CDS             complement(2136..2585)
                     /gene="rplI"
                     /locus_tag="ACHY3M_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4318479.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2627..2854)
                     /gene="rpsR"
                     /locus_tag="ACHY3M_003647"
     CDS             complement(2627..2854)
                     /gene="rpsR"
                     /locus_tag="ACHY3M_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4318480.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2859..3173)
                     /gene="priB"
                     /locus_tag="ACHY3M_003648"
     CDS             complement(2859..3173)
                     /gene="priB"
                     /locus_tag="ACHY3M_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFS4318481.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3180..3575)
                     /gene="rpsF"
                     /locus_tag="ACHY3M_003649"
     CDS             complement(3180..3575)
                     /gene="rpsF"
                     /locus_tag="ACHY3M_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4318482.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3902..4177
                     /gene="yjfY"
                     /locus_tag="ACHY3M_003650"
     CDS             3902..4177
                     /gene="yjfY"
                     /locus_tag="ACHY3M_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFS4318483.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4306..4992)
                     /gene="ulaF"
                     /locus_tag="ACHY3M_003651"
     CDS             complement(4306..4992)
                     /gene="ulaF"
                     /locus_tag="ACHY3M_003651"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFS4318484.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4992..5846)
                     /gene="ulaE"
                     /locus_tag="ACHY3M_003652"
     CDS             complement(4992..5846)
                     /gene="ulaE"
                     /locus_tag="ACHY3M_003652"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFS4318485.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5856..6506)
                     /gene="ulaD"
                     /locus_tag="ACHY3M_003653"
     CDS             complement(5856..6506)
                     /gene="ulaD"
                     /locus_tag="ACHY3M_003653"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4318486.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(6520..6984)
                     /gene="ulaC"
                     /locus_tag="ACHY3M_003654"
     CDS             complement(6520..6984)
                     /gene="ulaC"
                     /locus_tag="ACHY3M_003654"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFS4318487.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6994..7299)
                     /gene="ulaB"
                     /locus_tag="ACHY3M_003655"
     CDS             complement(6994..7299)
                     /gene="ulaB"
                     /locus_tag="ACHY3M_003655"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFS4318488.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(7315..8712)
                     /gene="ulaA"
                     /locus_tag="ACHY3M_003656"
     CDS             complement(7315..8712)
                     /gene="ulaA"
                     /locus_tag="ACHY3M_003656"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4318489.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9067..10131
                     /gene="ulaG"
                     /locus_tag="ACHY3M_003657"
     CDS             9067..10131
                     /gene="ulaG"
                     /locus_tag="ACHY3M_003657"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4318490.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            10239..10994
                     /gene="ulaR"
                     /locus_tag="ACHY3M_003658"
     CDS             10239..10994
                     /gene="ulaR"
                     /locus_tag="ACHY3M_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFS4318491.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10991..11740)
                     /gene="yjfP"
                     /locus_tag="ACHY3M_003659"
     CDS             complement(10991..11740)
                     /gene="yjfP"
                     /locus_tag="ACHY3M_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4318492.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QHNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11922..12251
                     /gene="bsmA"
                     /locus_tag="ACHY3M_003660"
     CDS             11922..12251
                     /gene="bsmA"
                     /locus_tag="ACHY3M_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFS4318493.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            12400..12675
                     /gene="yjfN"
                     /locus_tag="ACHY3M_003661"
     CDS             12400..12675
                     /gene="yjfN"
                     /locus_tag="ACHY3M_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFS4318494.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12792..14417)
                     /gene="aidB"
                     /locus_tag="ACHY3M_003662"
     CDS             complement(12792..14417)
                     /gene="aidB"
                     /locus_tag="ACHY3M_003662"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFS4318495.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(14501..15664)
                     /gene="yjfC"
                     /locus_tag="ACHY3M_003663"
     CDS             complement(14501..15664)
                     /gene="yjfC"
                     /locus_tag="ACHY3M_003663"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS4318496.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(15667..16305)
                     /gene="yjfM"
                     /locus_tag="ACHY3M_003664"
     CDS             complement(15667..16305)
                     /gene="yjfM"
                     /locus_tag="ACHY3M_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFS4318497.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(16315..16713)
                     /gene="yjfL"
                     /locus_tag="ACHY3M_003665"
     CDS             complement(16315..16713)
                     /gene="yjfL"
                     /locus_tag="ACHY3M_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFS4318498.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16731..17390)
                     /gene="yjfK"
                     /locus_tag="ACHY3M_003666"
     CDS             complement(16731..17390)
                     /gene="yjfK"
                     /locus_tag="ACHY3M_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFS4318499.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTIAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17441..18139)
                     /gene="yjfJ"
                     /locus_tag="ACHY3M_003667"
     CDS             complement(17441..18139)
                     /gene="yjfJ"
                     /locus_tag="ACHY3M_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFS4318500.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(18158..>18556)
                     /gene="yjfI"
                     /locus_tag="ACHY3M_003668"
     CDS             complement(18158..>18556)
                     /gene="yjfI"
                     /locus_tag="ACHY3M_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFS4318501.1"
                     /translation="TWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILF
                     TSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSL
                     KSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4764 a         4901 c         4382 g         4509 t
ORIGIN      
        1 cattgcgttt cagtggcgtt tttccactca accagccagg aagccatttt cccgtaaggg
       61 atgtatttat taccggcctc atgattctca ttctgacgtg ccagagcacg attgaaaacg
      121 aaacgacatg cgcctgcgaa gcgcctcatt tcacgctcct gttgaccacc gggtcttaac
      181 tggaatttaa atgcctgtag tcgttccaca ggtcaaatta tattttggtc tgtgaaaaaa
      241 gaaaccgata tccggcgtgg aaggcattgt gttttcctga tgcatgttca cctggtcttt
      301 gtcaccaaat accgacgcca gatttttgac catgacgcga cagaaaaact acgcacttac
      361 ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg cgaaccagat
      421 cacgtccatt tgttaattaa ctatcctccc aaactggcga tatccagtct ggtaaacagc
      481 ctcaaaggcg tatcgggtag gttactgcga cgagatcgac cagatattgc agtcaggtat
      541 tactacaaag gcgttttgtg gagtcctggc tattttgcca atagctgcgg aggtgcgcca
      601 atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc
      661 gccttatatc cccgccctga aggacggggt tttacggcgc accagataaa ccaggccaag
      721 cgtatgctgg ttgatcaaat aatatccccc aagcgcagca atattaatat tcacctgcaa
      781 tgggtcgata cctggcttaa aaatattgta tttcttccct tcatccaata aggattccat
      841 taactgcaag tgcgcatggt taatttctaa gagacgctgt gattttgcat aatgcacacc
      901 tttactttga ttttcactat gcacaatttt taaaaaccaa gggttagcaa tataataacc
      961 ccatgtaaaa tcaataattt tctcaatggc tttttgtggc tggttaacca gatgattaac
     1021 aaacgcaccg ataccaacag cgtagaataa ttacataccg cacaacaaga atgtatggtg
     1081 gaaggcgtgg aagtcatttt ctccccgggg gcatcgttat atccataatg agcgattttt
     1141 gatattaata tctttcacca tccgctattt tcactacttc agaaaacaaa cttattttta
     1201 ttaatatcga ttctttttct gtgaacatca gctgcgatat ttataaaaac attaaacagg
     1261 taacattaca atgacattac caacgactat ttattcattt cctgcatatt caccccattt
     1321 ttcgtccacc gacaaaccag taaaattaaa atttcatcaa tatgcacgtg ctacattact
     1381 gccaaatcgt ggaagagatc ataattgcga tggcagacgt actgttgaga tccacaaact
     1441 agatctctct gactggcaag cattcaataa gttagcaacg aagtgtaatg cttacgatgg
     1501 agtaacaatg aatggtgata attcttttgg atggaaccat gaagctacat tagataatat
     1561 tcacacccag aaatacaata aagctcacgc gggtgcgagg ttaaccgcag aactgaaata
     1621 tttattgcaa gatgtcgaat ccttcgaacc caacagtaaa tacacaatac acgaagtggt
     1681 tttgggacct ggttatggta ctccagacta taccggccaa acgataggat acgttgtaac
     1741 ccttcctgcc caaatgccta attgctggtc atctgaacta ccaacaatcg atctttatat
     1801 cgaccaactt cgcactgtta caggcgtttc taatgccctc ggattcatca ttgctgcatt
     1861 gctaaacgcg tattctgatt taccccatga tttgaaaatt ggtctacgtt ccttatcaaa
     1921 ttcagcagca atctactcgg gtttaggttt cgagcgagtg ccgcaggacc gagacataag
     1981 gagtgatcgt atgcatctaa cgccagcaaa ccatcctgac ttatggacac aagagaatgg
     2041 tgaatggatc tacttgcgaa actaatacct gacgaaaaat agaaagcaaa acgccgacca
     2101 atggtcggcg tttttacgtc tcgttgaata acgaattatt cagctactac gtttacgatc
     2161 actttcgcga atacttcgct gtgaacctgg aagctcactt cgtgttcgcc agtggtacgc
     2221 agaacgccgt tcggcagacg aacttcgctc ttagccactt caacgccagc tgcagttaca
     2281 gcgtcagcga tgtcgcgagt accgatggaa ccgaacagtt taccttcgtc gccagcttta
     2341 gacgcgatgg taacagtttc cagtgcattg attttctcag cgcgagcatt agcagctgcc
     2401 agaacttcag ccagtttagc ttccagttca gcgcgacgtg cttcgaagaa ttcaatgttt
     2461 ttcttggtag ctggaacagc tttaccctgc ggtaccagga agttacgagc atagcccgct
     2521 ttaacgttta cctgatcacc caggctaccc aggtttgcta ctttatcaag cagaataact
     2581 tgcattacct tatcctctca aagtcgtatt aatggaccgt gaccgattac tgatggcgat
     2641 cagtgtacgg cagcagggac aggtagcgag cgcgtttgat agcgcgagcc agctgacgct
     2701 ggtattttgc acgggtaccg gtgatacggc ttgggacaat cttaccgctt tcggtgatgt
     2761 agtttttcag cgtagcgata tctttatagt cgatctcttg aacgccttcc gcggtgaaac
     2821 ggcagaactt gcgacgacgg aaataacgtg ccatatggct agtctccaga atctatcaat
     2881 tcaatctgct cggcatgcaa aaccattttg ctcagtccgt tctttgcctt gtggcatgaa
     2941 atgaacccct gaacggttat gcgactgccg accgttatac tgtgagtaat ggcctggttt
     3001 tcgtgtccgc taacaataac gggcatttga caccatgcct gccggtgaaa gccggcttcc
     3061 tcctgcacag aacgatgctc aagcacgaac tggcagtgag gaattcctga tggactgacc
     3121 tttcgaaggg gagtcctgca cacggtgccg gacaacacca gacggttggt catcagaaat
     3181 tactcttcag aatccccagc ttcagcatca tcagcggttt cgtttgcgaa atcatcgcga
     3241 cgctcacggc gctcgtcttt cgctttaacc atcggagatg cttcggtaac agcgtgcttg
     3301 gtacgcataa ccatgctgcg gataacggca tcgttgaagc ggaaggtagt ttccagctca
     3361 tcgatcactt cctgcggagc ttcaacgttc atcagaacgt agtgtgcttt gtgcagtttg
     3421 ttgatcgggt aagccagctg acggcggccc cagtcttcca gacggtggat cttgccttct
     3481 gcaccagtga tggcagcagt gtagcgctcg atcatgcccg gaacctgttc gctctggtca
     3541 ggatggacca taaaaacgat ttcgtaatga cgcatcgaat tgctccttac ggattattca
     3601 gcctcctgtc tgggtcagcc gaatcccggg gaggcaagga acgtgttaaa ggtcggctga
     3661 aaaatgacgc gttatagtac ttgtatgccc cgctaaactc aaggccagcc gacaaataaa
     3721 tctcatcttt cgctcaaaag aactaactcc ctgatttgaa gttcatcaca ttccgtcaca
     3781 gtgttttttc gaacaactgc atcagaaatg gtcaccatca acttctttat cattgattaa
     3841 aattaaatca gaagatcatc acagaggaga gcacacaaag tcagccgtcg aggaggtatc
     3901 tatgttcagt cgtgttttag cccttctggc tgtgcttttg ctaagtgcaa atacatgggc
     3961 agccattgaa atcaataacc accaggccag aaatatggac gatgtgcaaa gcttaggcgt
     4021 gatttatatc aatcataatt tcgccactga aagtgaagca cgtcaggcat taaatgaaga
     4081 aacagatgcg cagggcgcaa cgtactacca cgtaattctg atgcgggaac cggggagtaa
     4141 cggcaatatg cacgccagcg cggatattta tcgctagcac caggtataac caacgaaaca
     4201 ttgccatagt ttgctttgcc cccttcgcag ggggcttttt ttgaaaaacg gctgcggaat
     4261 tagaccagtt atctcccgaa gaaggaaatt tccgcagcgc gtgttttact tctgcccgta
     4321 ataagcgtta ggaccatgct tacgcatgaa gtgtttattc atcaggaagc tgtcgatgtg
     4381 attgagttgt ggattaatac tgcgcgcaat ccacgccatt ttcgccactt cttccatcac
     4441 taccgcgtta tgcaccgcgt cgtgggcatc tttcccccag gcgaacggtc cgtgctgata
     4501 caccacaatt cccggcgtat gcagcggctc ggcgttgccc agcgtttcga taatcacttt
     4561 gccagtgttc agttcatact caccctgcac ctcttcttcg cttaatccac gcgtacacgg
     4621 gatgtcacca aagaagtagt cagcgtgcgt ggtgcctaac gccgggatcg ccagccccgc
     4681 ctgcgcccat gcggtagcat gagtggagtg ggtatggaca atgccgccaa gcgacggata
     4741 acgacggtag agttcaagat gcgtcgcggt gtcggaagat gggcgatact ccccttccac
     4801 caccttaccg ctcatatcga ccaccaccat atcgtctgct ttcatggttt cgtaggcgac
     4861 gccgctgggc ttgatcacca ccagcccgcg ttcgcggtcg atagcgctga cgttgcccca
     4921 ggtaaaggtc accagcccgt agcgcggcag atccatgttg gcttcaaata cctgctgttt
     4981 tagcttttgc attatgccgc ctccaccatg cccgctttcg ccatgcgcgc tttcacccaa
     5041 tcacgcgctt tcgccacttc tgccgccggg tcttccgccg tttcgctcca catctcaatc
     5101 aggtacggcc cgcaatagcc actctgtttg agcgtttcga aacaacgttc gaaatccact
     5161 acaccttcgc caaacggcac gtttttgaag acgccaggtt tggtgtcttt cacatgtacc
     5221 gcgacgatat gcccgattcc ggcctgcaac tccatctgca catcgttgtc ccacgccgac
     5281 aggttgccga tatccgggta gagctggaac cacgggttgt tgagatagtg cgcgtaaccc
     5341 agcgccttgc tgatggagtt catcaacgga taatccatga tctccatcgc cagcgtcacc
     5401 tgcgcgcggc tcgccatctc aacgctctct ttcaggccgt cacggaaacg acgacgcgtt
     5461 tcgttattgg cttcctgata gtaaacgtca tagcccgcca gctggatcac gcgaatgccg
     5521 acgtcctggg cgaactggat agctttacgc ataatctcca gcccctgcgc ccgcaccgcg
     5581 tcatcttcac tgcccagcgg gaaacgacga tgagcagaaa ggcacatgga cggcacgcgc
     5641 acgccggttt caacaatcgc attcaccagc gccagacgct gctcgcggct ccagtcgagg
     5701 cgcgacaggc gctcgtccgt ttcatctacc gacatctcga caaaatcgaa gcccaacgtt
     5761 tttgccagtt gcaggcgttc cagccagcac tccccggcgg ggagcgcttt ttcatagatg
     5821 ccaagcggga tttgtttgga caacatatcc gctccttagc cccacagttc ggcgatggaa
     5881 cgtttgaact gacgtgcagc ttcaaccgga gaagcggcat cacggatgct gcgaccagcg
     5941 ataaacacat gaatcggaat acctttgaac agcggcagat cttccagcgc caggccaccg
     6001 gtgacggtga ctttgaagcc catatcggaa agacgtttga tcgcggtaat gtccgcttcg
     6061 ccccacgcta cgcctgcggc ctgcgcgtca cggctgcggt gataaaccac ctgctgaatg
     6121 cctgcgtcac gccactgctg cgcctgttcc caggtccagt agccggtcaa ttcgatctgc
     6181 acgtcgccgt taaactcttt tgctacgtcc agcgcgcctt tggcggtgtt gatatccgca
     6241 cagcaaatta ccgtcaccca gtcagcgttg gcttcgaagc acatacgcga aaggattttg
     6301 cccgcatcag caattttagc gtctgccagt acgattttgt gcgggtagag cgctttcagg
     6361 tcacgaaccg cacgcacgcc ttcgcccacg cacagaatgg tacccacttc gataatgtcg
     6421 acttcttcgg caatcaggcg agtggtttcg taggcgctat ccatagtctg gttgtccagc
     6481 gcgacttgca acatcggtaa tgacatcttc aattccttct taagctgccg cgttggtgcg
     6541 gtcaatgaga tccagtactt cctgctcggt acggcaggca cgtaaacggt cgaaattttc
     6601 ttcatcttca aacaggttga cgatctgcat gatgcccact tcctggtgcg tgttggcatc
     6661 gaccgccgcc atggtgatga ggatatccac cgggtcgtta tcttcgtggt tgaactccag
     6721 cggctttttc agcgtcacca gcgcaaaacc ggttttctta acgccctctt ccgggcgacc
     6781 gtgcggcatt gccaggcctg gtgcgagcac aaagtacgga ccgtgctgtt caaccgcatc
     6841 cagaatcgcc tggtagtaac gcggctcgac aacatccgcc gccaccagca ggtcaacgcc
     6901 gatttttact gcgtcctgcc atgtctcagc ttcagcctgc aggcggatgg atttattctc
     6961 cgccagcgaa tcacgtaatt tcatggcgcg tccttacttc acatcctgcg ggaaatgcgc
     7021 tttgatcact tccagcagtt tcgggccaaa gtcggcagga gagagcatgt tgcgcacgcc
     7081 aaccacgtat ttgttgccgg tcacggtgat ttcgcccgca atgtgcgtag aagcgatgat
     7141 gatatccgcg ccgctcaact cgcttttgta ttcgccaacc gcgcagctat ttaccgtgtg
     7201 gtcaatgttt gattgggtta aaaactggtc cactttcatc ttcatgatca tggaactgcc
     7261 ttgtccgttg ccacacacag ccagaatacg tacggtcata atcaaaactc cttattaagc
     7321 agactgttct gccagttgtt tttctgcatc ttcttctgcg cgcagtgcgc ggccagcgaa
     7381 gaacatataa gccagtgcaa ttacaatgat gacggccata aaggcgatac cgatggagaa
     7441 gaagccctgc atcatcggcg gtgccagaat cgaccagtcc gccatgccca tccaggcgct
     7501 cataccggtg agtttcaccg cccagacgca gccaaagatt tcaatcatcc ccatcaccag
     7561 acatatcttc agcgccgcac gccagccgcc gaagtggtta gcgaacacgc cgatggtggc
     7621 gttggagaag aacatcggga taaagccggg aataatcagg atcgaggaac cgcaggcgac
     7681 caggatgcca accgcaatca gctgaccgat ggtgccccac ataaagcccc aaaccacggc
     7741 gttcggcgcg aagctataga tagccgcaca gtcaatcgcc agaaccgcac cagggatcag
     7801 gcgctgggag ataccgttaa atgcttcaga gagttccgcc acaaacatgc gcacgccctg
     7861 agtgatgatg aagatcgcca ccgcgaagga gaaaccagtt tgcaggatgt acaccgtcca
     7921 gttcactttg cctgccatcg cctgcacggt gtcgataccg aaggagagca gaatggcacc
     7981 aaagaagatg gtcatcacaa tcgccgtgga gacgatgttg tcgtggaaaa tattcagcca
     8041 gcctggcagt ttgaggtctt caacgctttc ctcttttttg ccgaggaacg gcgcaacttt
     8101 ataggcaatc catgatgcaa actgttgctg gtgaccgatg gagaaaccgc agccgtccgt
     8161 cacttcctga gtcggcttgt acatcatgtt ggaggtaatg ccccagtaga gcgaaaccag
     8221 aatcgccgtg cagataatgg ttgtccacat ggagtagccg aaaataaaca acgtaacggc
     8281 aatcagcccc gcctgctgga acatgatatg accagtcagc atgatggtac gaataccggt
     8341 aatgcgacgc agcagcacgt aacagatgtt cagcgccagc gctaacaaca ccgcgtaacc
     8401 tacccagcta taggcatcgc ccatgcggtc gatggttgcc atcatcgacg cgtaagtatc
     8461 ggaaattgcg ccgttaatgc cgtagacttc ggacattttc gccaccaccg gtttgaaggt
     8521 gctggtaagg atgccggacc ctgcctgcaa caacatgaaa ccaattatgg ttttaatcgt
     8581 gcctttaata ataacgctga cacttttgcg cagtaggatg tagcccagac aggtcacaat
     8641 acccagcaac aacggggcat tggtcatgac ctggttaaaa aacacggtaa agatgttgta
     8701 gaggatctcc ataacgatct cctgaagaag aggtaaccgg gtattccgca cacccggaat
     8761 gttgtgcact cactctaatt ttcaaaagta atcacaacaa gattatttat gattaaatgt
     8821 gacgcgaccc gcaaataatt ccacacggat agagtgggat tcacacaacg gaacaattat
     8881 ttatttaaat tcatttagtt aatttaacta cacattccat taaatctttc cacactcaaa
     8941 atcaacaaca acttccctgg caaaaagtca aaaaatggtc attgccttcc tgaaaaaata
     9001 gtgtcaggat taatcaaaac caatcacata ttgattcgaa ttgaactatc atgaaggtaa
     9061 atggcgatga gtaaagtgaa aagtatcacc cgagaatcct ggatcctgag cactttcccg
     9121 gagtggggta gctggctgaa tgaagaaatt gaacaagagc aggtcgcccc tggcacattt
     9181 gcgatgtggt ggctaggctg caccgggatc tggttgaaat cagaaggtgg caccaacgtt
     9241 tgcgttgatt tctggtgcgg cactggcaaa caaagtcacg gtaacccgtt aatgaaacag
     9301 ggtcaccaga tgcagcgcat ggctggcgtg aaaaaactgc agccaaacct acgcaccacc
     9361 ccgtttgttc ttgatccgtt tgcgattcgc cagatcgacg cggtactggc gactcacgat
     9421 cacaacgatc atatcgacgt taacgtcgct gctgccgtga tgcagaactg tgctgatgac
     9481 gtaccattca ttggaccgaa aacctgcgtt gatttgtgga ttggctgggg cgtaccgaaa
     9541 gagcgttgca tcgtggtcaa accgggcgat gttgtaaaag taaaagacat tgaaattcat
     9601 gcgcttgacg ctttcgaccg tactgcactg atcaccctgc ctgccgatca aaaagcggct
     9661 ggcgtactgc cggacggcat ggacgatcgc gcggtgaact atctgttcaa aacgcctggc
     9721 ggctccctgt atcacagcgg cgactcccac tactctaact attatgcgaa gcacggtaac
     9781 gaacatcaga tcgatgtggc gttaggatcg tatggtgaga acccgcgcgg tattaccgac
     9841 aaaatgacca gcgccgatat gctgcgtatg ggtgaagccc tgaatgcgaa agtggttatc
     9901 ccgttccacc acgacatctg gtcaaacttc caggccgatc cgcaagagat ccgcgtgctg
     9961 tgggagatga aaaaagatcg tctgaagtat ggcttcaagc cgtttatctg gcaggttggc
    10021 ggcaaattta cctggccgct ggataaagac aacttcgagt accactatcc gcgcggtttc
    10081 gatgattgct tcactattga accggatctg ccgttcaagt cattcctgta atctgtcgtt
    10141 tataccggat agcgtggaga agttcgcgct atccggcgta gtatttcctg gctatttcaa
    10201 atatcatcta aaaaaatcaa attttatcgg aatagctcat gactgaagca caaagacatc
    10261 aaatcctcct ggaaatgctc gcacaattgg gctttgtgac cgttgagaaa gtcgttgagc
    10321 gtctgggaat ttcgcctgcc actgcgcgac gcgatatcaa taaacttgac gaaagcggca
    10381 aactgaaaaa agtgcgcaat ggcgcagaag ctattaccca gcagcgcccg cgctggacgc
    10441 cgatgaatct gcatcaggcg cagaatcacg atgaaaaagt acgtatcgct aaagcagcct
    10501 cgcagctggt taatccgggc gaaagcgtag tcatcaactg cggctccacc gcgtttctgc
    10561 ttgggcggga aatgtgtggc aagccagtgc aaatcatcac taattatcta ccgctggcaa
    10621 attacctgat cgatcaagaa catgacagcg tgatcattat gggtggacag tacaacaaaa
    10681 gtcagtccat cactttaagc ccgcagggca gcgaaaacag tctctatgcc gggcactgga
    10741 tgtttaccag cggaaaaggg ctgaccgcag aagggttgta taaaaccgat atgctgacag
    10801 caatggcaga gcagaagatg ctgagcgtgg tagggaaact ggtggtactg gttgatagca
    10861 gtaagattgg cgaacgcgcg ggaatgcttt ttagccgtgc cgatcaaatc gatatgctga
    10921 tcaccggcaa aaatgctaac ccggaaatcc tgcaacaact ggaagcgcaa ggggtcagca
    10981 ttctgcgtgt ttaaagatgc tggcggaaaa atgtcacggc agcatctaac gcctcaggcg
    11041 taatgcggtg acgcacgcct ggctgccatg aacaggttag cagtttatcc cgtcccgtct
    11101 cgcttaaggc ctgctgcaaa cgtagtgatt cgtcggcagg cacaacgtca tcgtcgaggc
    11161 catgccacag cagtagaggt ctgtccccaa gttgttccag gtggtttgtc gcttcccact
    11221 ctgccagcgg cgcgataatg ttattgaatt cattgtgctg tgctgccgtt tcaggtatca
    11281 gcggtggaaa cagtgaacgg gcgagcgatg taaaatagcc cgatcccatc atgctggcgg
    11341 tacatttcac cgtggggtgg cgagtggtaa ttcccagtgc cgtcatcgcg cccatcgacg
    11401 cgccaccgac tgccagacgg tcatcaagca gccagttttc ttcggctatt gccgcacgta
    11461 aagtagtgaa ttcctgcata ctttgtagca agatttgcca gaattgattt aaccgccgcg
    11521 cttcgtcgct actaaaacgg ctaccgtgat cgggcgcatc cggcatgatc acccgcaaac
    11581 cagcctgcgc cagcgcaacg gcaaaatagc tatacaccag gctggatgaa gtaaagccgt
    11641 gataaaaaat tacgcacggt aacggggtat ctttttgccc gacaggataa gcatgaagaa
    11701 cgggaatatc tgccagctcg cgtgattcta tttcaatcat ctgtctctcc tttttgtctg
    11761 gtatgacatg aagaaatcct tagcacaagt ttctgctggc ggtattagtt cataatgttg
    11821 agatgtgggt tacgctttcg tcacgttttc attcgaaaat cgcgtaccag gtaacaattc
    11881 gggaacattc ccccaaagtt aaattaataa ggcactacac tatggttagc aggaaacgta
    11941 atagcgttat ttaccggttt gccagtttat tattggtgtt gatgttaagt gcctgtagcg
    12001 ctctgcaagg tacgccacag ccagcaccgc cagtaacgaa ccatccgcaa gagattcgcc
    12061 gcgaccagac gcaagggtta cagcgaatag gtagcgtaag cacgatggtt cggggttctc
    12121 cggatgacgc attagcagaa atcagagcaa aagctgtcgc tgcaaaagct gattattacg
    12181 ttgtcgtcat ggtggacgaa accatcgtga caggacagtg gtattcacaa gccattttgt
    12241 accgtaaata aatcagacaa cttacatagt cagctttaca ttgctttgcg tccctatgcg
    12301 tttacctgtg cacaataaat taccggctgg aagcctgggc gtttattacc gcgatggaat
    12361 gccctgcaac atgtggggaa acgaaaaatg gagctgacga tgaaacaatt acttgcctca
    12421 ccctcgctgc aattagtgac ttatcctgcg agcgccacgg cacagtctgc agaattcgcc
    12481 agtgctgact gcgtgacggg cctgaatgaa ataggtcaga tctcagttag caatatttca
    12541 ggggatccgc aggacgtgga acgtatcgtg gcgttaaagg ccgatgaaca aggtgcgtca
    12601 tggtaccgca ttattaccat gtacgaagac cagcagcctg ataactggcg cgtacaggcg
    12661 atactgtatg catgagtgtt atatacagca aatgaacaat gccggatgcg gcgcgagcgc
    12721 cttgtccggc ctacaatttt cgcatatttc attgagttaa tcgaaaccag cgtcgcatca
    12781 gtcgtatacg cttacacaca cactcccccc gtcgcccgca gcaataaatc attctggatc
    12841 tgctctgaca accgtacgcc gccgcgcgta tctaacatca cctgacacca cgcctgcgcc
    12901 attggcggag aggcatattt caacatttgc gcaccgcagc ccagcaggaa tagctgatga
    12961 gtaatctctc gccccagttc ttcagctggc ttgcgcagct gctgctgtaa acgacgaacc
    13021 gcgcgatcaa aatagcgatc ctgccctttc acttccacaa acgcttccga caataagtcg
    13081 tatacgcccg cttgcttatt gagaacgcgc agcacatcca ggcacataat attgccggaa
    13141 ccttcccaaa tactgtttac cggcatctcc cggtaaagcc gcggcagctc gctctcctcg
    13201 caataaccaa tgccgcccag cacctccatc gcttcggcca caaacggcat acctcttttg
    13261 cagatcacaa attttgccgc aggcgtaaat aaacgcgccc acagggcttc tttggcatcg
    13321 gcacgccggt cccacgctcg cgcaagacga aacagcaacg ccgtttgccc ttcaagctga
    13381 agtgccatgc gacttaagac atgacgcata aggggctgtt ggatcaatgg attaccaaaa
    13441 acatggcgtt gatgtgcatg ataaatcgcc agcgaaaatg cacggcgcat catggcatgg
    13501 ctacccaggg cgcaatcaaa acgcgtcatc ccacccattt tcaggatcag acgaattcct
    13561 tccccttcct gccccaacaa ccagccaatg gcatcctgaa actccacttc gcagctggcg
    13621 ttagagcgat tacccagctt atctttcagc cgctcgaggc gaatcgcgtt gcgttgcccg
    13681 tcaggcaaaa agcgcggcac aaaaaagcag gacaaaccgc ccgtagtctg cgccagcacc
    13741 agatgcgcat cgctttgcgg caccgagaaa aaccatttat gccccaccag ccgataagag
    13801 ccatcttcca ggcgctctgc gcgggtggtg ttgctcatga catcggaacc gccctgcttt
    13861 tccgtcattc ccatgccaat caacaaaccg cgtttttgcc cacctggcaa taagtgagaa
    13921 tcgtagcgat cgctcagcag cggcgtggtc cagtcctgaa acggcgtggg taacatctgt
    13981 aacaacaatg gcgtggcggc aaaggtcatc gttattggac ataacgaccc agcctcaacc
    14041 tgcgcatgta acataaaacg cgccgcgcgc gccacaaatg cgccggagcg agcgtcttct
    14101 tcccaggcaa gattgtgcac ccgattggta cataacgcct gcatcagcag gtgccaggcg
    14161 gggtgaaaac gcacatcgtc cagacggcgt ccttgcgcat cgtagcgcaa taattcaggc
    14221 ggattcacat tcgccagccg ccccagttca agagattcag ccgttcctaa ctgctgacca
    14281 atactggcta gaaaatcgct atcccagcca gcaccttcac gcgttaccgc ttcacagagc
    14341 gcgccatcag acaggtataa gttgctgtta tttaatggta taggttgatt aaaaacggtg
    14401 tgagtttgcc agtgcactgt gtctccctcc atcaatggca gtcaccctta gtatggtcac
    14461 tgccatggat tcctgacaga aagagattgc taaaacattc ttatccagca atgtaatgcg
    14521 gaacgaaacg tgaggtgtct ttggtgatca gcgtgttatc ttcacggatc cccattccgc
    14581 acgcttcatc atcgacaatc cagctaccga tgagtgtgta gctatcgcca aatcgcggca
    14641 gaggttgaaa cgcctggtag atcatcggtt catcggcgta atcaccatca gcgtggtcaa
    14701 cgacgttatt ctgaccgtcg aaaatggtga cgtttccgcc ttcgcgcgag tagatcggtt
    14761 tacgcacata gctttcgcca gcggcaatct gcggtttttc gccctcaaac caggatgcaa
    14821 gaaggttagg atgaccaggg aagaagcgcc aaagcagcgg cattagccct ttattactca
    14881 agatactttt ccataacggc tccacccatt gctcgcgacg cttgcgcagc agcggaccgt
    14941 tatcgtcacg catcatccac tccagcggat acagcttaaa tgcacgctgg atgacattat
    15001 catcaagatc ggtcagtaca ccgccgacgc ccaaaccgag atcttcaatg tagataaacc
    15061 gcgactcctg ccctgcctgc tgggcgcagt cctgcaagta cagcacggta gtcctgtctt
    15121 catcggtgtc ctgacagcag cagaaataaa acggctcccg gctataaagc tcgctaaagc
    15181 gtgaaatcag gcgttcctga atggcattgt actgatcggc atcacgcgga ataatgccgc
    15241 tgcgccgggc atcctccagc cacaaccact ggaaataagc cgactcatac aacgaggtcg
    15301 gcgtatcggc gttgtactcc agcagcttca ccggcgcatt gccacaccag gcaaaatcca
    15361 tccggccata cagcgaagga tcgcgtgcgc gccagctttc ggcgatcaca tcccagtaca
    15421 acggcgggat cgccagttgc gtcaggatct cttcgtcttt aaccgcgcga tcaaccacct
    15481 caaggcacat ctgatgcagt tccgcagtcg gtttttcgat ctgctcttca atctggcgca
    15541 gagtaaaacg gtaagcccga ctctcgtccc aatagatttc attgtcgatg atatgaaagt
    15601 cgaaaccgtt gttagcggcg atctggtcca gatcccgtcg cacaggaacg ttgtgtctca
    15661 gcatgattag cctccccaat gcccacgggc gctggaagaa cgaccatagc cgccgcgcga
    15721 aacggttgac gcttttttgg tggtaaagcc gcctgaagag taagactctt ttttgccaga
    15781 accggagcgc caggagtaat caccggaagt gttgcgccag acggggcggg aagcaaatga
    15841 ggatccgccg ctgttataga cgaacggctc atcgcgatct ttacgaataa cccggcttaa
    15901 caaaaatcca gaaacgaccg ggatccaact ctgttcaaca ttgtcgtagt agcaattttc
    15961 gtacttagac tgacaattct gctgagtcat attcttcgga acatcggcat aaaatgccgc
    16021 tttggcgttg ttccagccac gcgcgcaaat gtcggcatta ttaccgtcat caatacaatc
    16081 ctgcactgtc gaataaaacg ttccgtcgcc gtcgttatcg acatcgccac tatcgccaca
    16141 acctttcaat acaaaaaaag cggcaccgcc cattagagcc aacgttaagt atttttgtgc
    16201 gtagcgattg cgacgcggtt caaaaggatt attcggtcta ccaatgcgac tgatcgctcc
    16261 gtgaccaatt ttactgttat ttcttgattt gcgttttctg gccatgatcg tccattacca
    16321 tgtcatacaa gctgcgttaa aaataccgcc agccagcgcg gcggttccca taaacattcc
    16381 tgctgcggta ttgtgattaa taattttttc gcttaatgcg ggcatataaa gtctgacacc
    16441 agcaaaaacg agtaactgaa tcaccagtgc aatcccgccc caggcgaaat agtctggaat
    16501 actcaccgca ttgattgccg cactggataa tgggataacg taacctaaca atgtaccact
    16561 gaacgccagt gaagccgcgg tattattgtt tttgattaac tgccattcgt tgtgcggtgt
    16621 aattttagaa tagataaaaa gaaaaataat caccatagcc acgccaataa aaaaataggc
    16681 gctaaaggca agaagtgaat ccagtatatg cattgtaaac gtccttgtaa ttaaccaata
    16741 atatgaagtg atgtcagcgg gatatcgaca cccaacgcgc gcgaaaataa ccactctggc
    16801 tcgccgagat cgttaaaaga ttcctcacca tttaacagca aatattcgta agtatcttcg
    16861 gttacttgcc gctggtagcc catggtaaaa ttatggacct cccatttggc atggttttga
    16921 ttttctactt tttccagcat gtagacgggt tcgatatttc ccggttcttc gctgttaaaa
    16981 aatcgctgcc agcgtttttc ctgccagttt aagatcattg cccccatcac tttggcgttg
    17041 atcgcctcgc gccagtgact ctctttactg ataccgtagc tctcttcata gacaaagagc
    17101 ttgatatcat caatgtcatc aatatcttcg ccgccggtgg tattgatttg cagaaattca
    17161 tcgcctgaag tgtagtagcg gaaaatctga ctaccgccgc ccagatcgat gtggctgaca
    17221 gcggctatcg taaattcctc acccggcagc gcaatcagca attcatcttc cagcaaacga
    17281 aacgctaacg tgtcgagcgt aaagccacta ttgagatgaa gtcccagcgg accgcgagcg
    17341 atagcaggct tattatcctt gccaaacaga cgctggaaaa aaccagacat attttatcct
    17401 taaaatagtt gccagccttt tagcgaggct ggcaaaaaaa ttactcgccc tgttggcgtt
    17461 gcagtcttgc taatacatcc tgggcgctac ttttattgct accgccaatt ccggcttccg
    17521 ccagcttttc atcaagatcg cgaccgtctg cgactttttc caactgtgca gcagcatcca
    17581 ggcgagcctg acgttcggcc tgacgcgttt gcaggcgttt taaggattct gccgctgtcg
    17641 aaacgctgga agatgcgcca acggtagagg ttgttacagc ctgttgtgca cgctgcatgg
    17701 cttcagtggc tttaacgact tccatttgct gctcaaactg agcgatacgc tgcgctgtcg
    17761 ctgtaactgc tttttccacg ccatcacggg agacttccag attcgacaac acttgctctt
    17821 cagcggtaat gagattctca aggcgcgcga tttcttcagc aacttcgtta atcaacgacg
    17881 gattaacgtt cttgcttaac gcttccagcg cacgggcttc cagactggct ttgcgctcac
    17941 gtaaatcttt cagcttatcg tgacttaatt tcacccgcgc cagcagatca acgcgagatt
    18001 ttccggcttt atcgagttca gctttagcgt cacgaatatg ctgttccagc atgcgcacgc
    18061 cctgggtttc ttcaatggat tcttccgcct gggagataaa cgatttcccc agcgtaaata
    18121 aactttttaa aattcccata aatacccgtc cttgtaatta gtgtgaatat tcttctgtaa
    18181 tttcagccag atccaatgcg ttatctacca gcgaggttat ctcaagcagg atatcttcaa
    18241 gagaagattt aagcgataac gccccgaaaa caatgtaata ctcttcctgt tgcacgctgg
    18301 agattcctac cgatgacaac ggcatcatct tctgatttct taataagaag gtattaaatt
    18361 catctggatt gctgatacta cttaccggac aaataaaggt ttcgataatc atttggcggg
    18421 aagtaaaaag aatattgatt tgcaaatcgc cataatcatt cattttaata attaatgcgc
    18481 tctcgctatt tgtcacatca atattttgtt caggtacagt ttgcagcgcc gtcgccagcg
    18541 ccaacggatt ccatgt
//
LOCUS       DAZUFB010000094        18498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZUFB010000094 DAZUFB010000000
VERSION     DAZUFB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.094"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..631)
                     /locus_tag="ACHY3M_003669"
     CDS             complement(53..631)
                     /locus_tag="ACHY3M_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4318502.1"
                     /translation="MTFRMSEQSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEE
                     ASLLTAWKTYRVLLNRVDTTVASDVEWPVAPQ"
     gene            complement(631..1485)
                     /locus_tag="ACHY3M_003670"
     CDS             complement(631..1485)
                     /locus_tag="ACHY3M_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318503.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALI
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGAHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGGHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNAENTVKNIA
                     FNYIVRLA"
     gene            complement(2050..2763)
                     /locus_tag="ACHY3M_003671"
                     /pseudo
     CDS             complement(2050..2763)
                     /locus_tag="ACHY3M_003671"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(2766..3296)
                     /locus_tag="ACHY3M_003672"
     CDS             complement(2766..3296)
                     /locus_tag="ACHY3M_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFS4318504.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(3289..4185)
                     /locus_tag="ACHY3M_003673"
     CDS             complement(3289..4185)
                     /locus_tag="ACHY3M_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFS4318505.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(4189..4539)
                     /locus_tag="ACHY3M_003674"
     CDS             complement(4189..4539)
                     /locus_tag="ACHY3M_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFS4318506.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(4536..5102)
                     /locus_tag="ACHY3M_003675"
     CDS             complement(4536..5102)
                     /locus_tag="ACHY3M_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS4318507.1"
                     /translation="MRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(5114..5749)
                     /locus_tag="ACHY3M_003676"
     CDS             complement(5114..5749)
                     /locus_tag="ACHY3M_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS4318508.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELWGISQADERLIYNTVLGRIAEAVR"
     gene            complement(5742..6209)
                     /locus_tag="ACHY3M_003677"
     CDS             complement(5742..6209)
                     /locus_tag="ACHY3M_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318509.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSPEGDWSHVFQDVTWGESDG"
     gene            complement(6347..6754)
                     /locus_tag="ACHY3M_003678"
     CDS             complement(6347..6754)
                     /locus_tag="ACHY3M_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFS4318510.1"
                     /translation="MKLWPTLGVAFLLIAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGGQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(6751..7143)
                     /locus_tag="ACHY3M_003679"
     CDS             complement(6751..7143)
                     /locus_tag="ACHY3M_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS4318511.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(7140..7463)
                     /locus_tag="ACHY3M_003680"
     CDS             complement(7140..7463)
                     /locus_tag="ACHY3M_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4318512.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(7466..7666)
                     /locus_tag="ACHY3M_003681"
     CDS             complement(7466..7666)
                     /locus_tag="ACHY3M_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFS4318513.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(7666..8160)
                     /locus_tag="ACHY3M_003682"
     CDS             complement(7666..8160)
                     /locus_tag="ACHY3M_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFS4318514.1"
                     /translation="MSMVARTEPRPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(8263..9063)
                     /gene="gpM"
                     /locus_tag="ACHY3M_003683"
     CDS             complement(8263..9063)
                     /gene="gpM"
                     /locus_tag="ACHY3M_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS4318515.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLTDADNMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(9109..10161)
                     /locus_tag="ACHY3M_003684"
     CDS             complement(9109..10161)
                     /locus_tag="ACHY3M_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFS4318516.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(10185..11021)
                     /locus_tag="ACHY3M_003685"
     CDS             complement(10185..11021)
                     /locus_tag="ACHY3M_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFS4318517.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSVQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11176..12927
                     /locus_tag="ACHY3M_003686"
     CDS             11176..12927
                     /locus_tag="ACHY3M_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFS4318518.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGHPDVMAAGKKG
                     RAKKLKNHFTPEQISALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRRRFSVDWNTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            12927..13973
                     /locus_tag="ACHY3M_003687"
     CDS             12927..13973
                     /locus_tag="ACHY3M_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4318519.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAYLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGSGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(13988..14512)
                     /locus_tag="ACHY3M_003688"
     CDS             complement(13988..14512)
                     /locus_tag="ACHY3M_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318520.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVHLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLETTEGFRRMPLSQIPASTPKARGGNDERKLIFSIETV
                     VDDTPGEVAFVHTPLSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            complement(15709..16020)
                     /locus_tag="ACHY3M_003689"
     CDS             complement(15709..16020)
                     /locus_tag="ACHY3M_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFS4318521.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKTNPAEAYRLAAWADDEMLPVTQISSKKFEAQSAPVAKITSQMAGNIRALFP
                     E"
     gene            complement(16025..16984)
                     /locus_tag="ACHY3M_003690"
     CDS             complement(16025..16984)
                     /locus_tag="ACHY3M_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFS4318522.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDIANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYNSLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFITDGVPHFALVNGLYAMNKE"
     gene            complement(17061..>18498)
                     /locus_tag="ACHY3M_003691"
     CDS             complement(17061..>18498)
                     /locus_tag="ACHY3M_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFS4318523.1"
                     /translation="EDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVD
                     GIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQ
                     MARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELN
                     DYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPETEDSARENGFDLQGGPAAPW
                     TRGNNCPRVQETDNNGAEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDK
                     QAKNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSL
                     YVDGSIIAPQGYEIVRKPDTRPDSRITQLWQHLSRNHGVSSTEIRHNPVASYLAQLGA
                     SDPEAAARLASALQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLQ
                     GGLTSGNKKQTETGLTNPVNEQKTRSDI"
BASE COUNT         3850 a         5045 c         4966 g         4637 t
ORIGIN      
        1 tataaataga gtacatgaag ccatcaatat ttctatcgat ggctttatcc ttttattgcg
       61 gggcgactgg ccactcaaca tccgatgcta ctgttgtatc aacacgattc aacaataccc
      121 gatatgtctt ccatgcagtc agtaacgatg cctcttcctc cgtcgcaata tccaaatcta
      181 cggcatcctg aagtggcgca atatgctcac tggccatctg catcaggctg ttttttgttt
      241 cttccgcctt ccggatccgg aacagttttt ctgcttctgc atctttcacc caggctgtgc
      301 cgttccactt ctgaaactcc ccttccgggg ataaccaggt gacattttcc ggtaatgggc
      361 caagttcaga aataaataac gcgtcgccgg aagccacgtc atagacggtt tttccccgat
      421 ggtcttcaac gagattccac gatgcctcat cactgttgaa aacggccacg aagccagccg
      481 gaatatctgg cggggcaata tcggtactgt ttgcaggcag acctgtatga ggtggaatgt
      541 atgcatcacc ttcaccaata aactcattgg ttccggtcag tagattataa atttttatgg
      601 tccgtgattg ttcactcatt ctgaatgtca ttatgcaagc ctcacaatat agttaaatgc
      661 gatgtttttt acggtgtttt ctgcgttccc cgtagcatta acggtgatgg tgtgtccgtg
      721 gggaccaata atgacagaat gggcatgagc accaatgccg atagtgtggt tatgggcacc
      781 gatatataca ttgtgagcat gccctcctgc gctcgacgtg gtacctgtac ctaacgcagc
      841 gttataaccg gctgatacac cattccccgc gccagtgtga ccaacaggta tattgtgtga
      901 atgagctcct gcgctatttg tggtttttgt gccgtaatca aatgaactgg tcgttttagt
      961 accgtaatca aacgacgatg tggttttcgt ccccaaatca gtactggatg cgctggcgct
     1021 gtgggtgtgc gatttaatgc cgtcctgttc ctgagacaat acggcacgac cactggcagg
     1081 tttgcccttg atagtccagc cacgcatatc aggaataacg cctgacggat aagcgactgc
     1141 aagtttcggg tatgcagatt tgtcaaaagt ctgcccctgt atcagggcat aaccagacgg
     1201 aacggtatct gatggccacg gaatcggtgc accgactgga taaaactctt caggaggatg
     1261 agccgaggtg taaagctgcg cccacggcga ccagtttgcg tcggtcgtat cccgtcgtga
     1321 acgaataaat gccggagcat gagcaccgct tataccactc cagccgatga gtaattcgcc
     1381 ttcgccaacg gctgtcatcc ctttcaggtg aatgatattt ccatacgttg ttggatatcc
     1441 gttgttatac gcctcgtata actccagacc tgtggccccc tgcatattat ctgtcagggc
     1501 ggtcagccga ccttttgaag ccagattaac tgacgatact gctgtcccat ctgacggtaa
     1561 cgccccgatc tctgatgctg ttggcttatt cctggagtta tagtcccttc gccagccagg
     1621 agcataagca tcaccatgat taatataagt aaattgagca ttagggatgc cttcaccgct
     1681 ggatgtactc ggtgtggtaa tgcgtatggt cattgcgccg cgggtgccaa taacttccac
     1741 aacagcacct gcaagacaaa tacttccgca acctgtatct gtaatgacct tattatttgc
     1801 ataagcccat gagcctttgc acatccagta aggatggtta aatgccccct gactctctag
     1861 ccaaaaaatc aactgcgcag ttgtccatgc ttcactatct ccaccaatat tcagctctgc
     1921 actatatgca cggcaggcac cgatattttt cgtaaaggtg tctttatcag agatatcctc
     1981 accattctta gctttctgca gacgtttttc agcattgtca taggcagctt ttactgcatt
     2041 tgaagttgcc gccagcgttt cagatgtact gttggttgca ctactaagct ggacaaggcc
     2101 ttttcgcgct gttgtggcat cctgcgctgt atattttccg ttagcaagat catacgcgac
     2161 cttaaccgct ttcggcgttg cagccagcgt ttcagaatcg ctgttagtgg cgctactgag
     2221 ttgaacaaag ccttttgcgg tcagcgaggc atccgggtga cgtcgtgact gttcatgctc
     2281 tttcagtttg ttatccacgt aatccactgt ggccatcacc atggtgttat ccacggtaag
     2341 cgccacagtg gcagtgctgg atacggtcag aatggtgcga aatgtttgtg cacgtccgga
     2401 cccttcggca acggctggct tgtaactttc ggcagtattg cccaccgcga ttaaatcgcc
     2461 gtgctcatca aacacaccaa tttcccggat ccagaatccg cccgtttctg gaggaataac
     2521 cagctccgca ataatgcggt tctgatgtgt tgcgtccagg atgacgcgat taacagtgtg
     2581 tcgccacacc tcatgcacca gacgggtctg cttactgtct ggtgtgggca acgcgccgcc
     2641 accgtcgccc acggccatat gagtcaggcg gacaggctta ccatctggcg cggctgcctg
     2701 agctaatttt ttggcacctg tatcggtgat aacggtttta aattttcgtg ttgtggtact
     2761 catgcttaat cgtccggata aatggtaata acttcaccgt cataagttgc cgccgctgca
     2821 aaaatatccc ccggaatttc ctgaatgata ttcagccctg tcatgtggcg gctgaccgga
     2881 cgggcatcag caatcaaccg ctccatttcc agatacattt cctccgtcac gccactgtcc
     2941 agtgtgccga cttcaacggt aaatgttccc ggttctccgc cgaactccca ccactcagac
     3001 acgcgaatga ggtatcccag cggctcaatg gccctgcgca gtgcgctgat ggtccctttg
     3061 tgtcggtgta tcagccatgc atcacgaatc acctgtcgtt ttgtctcttc cggccagttg
     3121 cgatcccagc ggtcaacgga aaatgcccag gcgagataag gcagcaggtg caccgggcag
     3181 gtgtccggcg accacagcgt gttgaggtct accgggatgt ctgtaatgcg cgttccgaca
     3241 gcttcggcac aacgcatgaa actgctggct gatggtggta acagtgaatt actcattgcg
     3301 cccaccttcg ctgatggtga acgactcaca gcgcgccgcc tgtatgtcgc tgatggccat
     3361 attctgtgtg ggttcgatta tctccacgcg ttgcacaccg tgcacatgca gtgcggcagc
     3421 aatggcggac aacgccacgt cctgaccgat aagcccctgc tcagccagcc acttcctgaa
     3481 cgacgattca gccgccgcca gaataggttc ggattccggg ccggggtaaa agtacagttt
     3541 tgcattcagc cgccatgtca cgattctggc gctctgtaca gtaaggcggt cggccaccgg
     3601 gcgggtatcc tctgcattca gaacggcgcg aacggtatta agcaacgcct ccgttgctgt
     3661 gccgtcgcct tcagtggaca ggatggaaac cgtcacattt gccggagacg gactgatagc
     3721 ccgcgcatca cgcaccagac cgctggcgct gcgggcaaaa tactcgtatg cacctgacgg
     3781 gccagcaaca ctcaggccat cgtacgcccg ctgcgcccgc agtctcagcg aggtgtcact
     3841 ttccatcact gcgtcggtgg tatccgttgc cggagtgatg gtcaggcgct ttgtgttcat
     3901 attgcccgcg aggttgtcca ggtctgtccc ggcgctgtgg cttaacatgc aggcgcgtgc
     3961 cccctcattg acccgctggc gtaacagcat ttcacgaaac gctgttgtct gggcgataac
     4021 gttcaggggt tccgattcca gctccagcgc ggcggagacg gcttcacgct gttcggcggg
     4081 ataagccgca atcatcatgg cctttgtgtc agccagaatt gcctcaaagt caggctccgc
     4141 gatgatggcg ggttccggta actgggaaag gtcaacggcg ggcatgattt actccctcag
     4201 cgtgatggtt aactcaacat tctgcatggt ctgcatgaca gtgcccgaca gcgtcacccc
     4261 ggcgcggcct cccgctttcc agacaacgtc gatggcatcc agggcaatgc ggggttccca
     4321 tcgtgtcagc gcaatcacgg cagcactcat gcattgcaga cgcgtggtgt tattcatggg
     4381 ttcgtcaatc aaatcaggca caaggctgcc atattcccgt cgcataaccc ggcttgccag
     4441 cggggtggtc aggatatccc tgactgactg tttcaggtgc tccatatcgt tcaggtttcc
     4501 cgtcccgtcc ggattcattc ctgtgtagcg ggttgtcact gcgggcctcc tgtcgaatcg
     4561 ctgccgcctt tcacgccacc gtgtttatgc gtatgcacgg taatgccgtt tgaggtgaag
     4621 ttgccgccgc tgtgcgtgat attgccgctc atctttcctc cttttgtgac gtcaagcgtc
     4681 gctgttctca gaaggtctgt gcattccacg acaggtgtgt ccagcgtcac actgacggat
     4741 gcctgcaggg tggccgtttt catgccgctg gcgctcagtg cgcctgcgtc cgcgtcgtag
     4801 cggaacaccg cgccatccgg cgcgctgacc acgatttctt tcaggctttt gccgggtgcc
     4861 ggaatggcat cactccacag actgccaatt atcatggcgg tttccgggtt gccgccgatg
     4921 caggcaatta ccacctgttc gccgggtgat ggcggcaacc acacattgaa ggcacccgcg
     4981 cgcgtggtgt tccagcgcag ccagcctgtt tccagttcgc cgctgcgaac gcgcacgcac
     5041 caggattcct catcaacttc agagatgatc ccggtacgga tgatgttgct cagcagtcgc
     5101 atgagttcag cgctcaccgt acagcctccg caatccggcc cagcaccgtg ttataaatca
     5161 ggcgctcatc tgcctggctg ataccccaca gctcacgtac cgggtaatcg gtgaaaatgc
     5221 ccggcgcaac ctgatcgcgc tcaccgaact gatgaacgcg ggcaatacgt gcggccacgc
     5281 cgctgtaacc caccgtcaca ccggaagcat ctgcacgggc tttcaggtag cgggcggtgc
     5341 gcagttttac gaacatgggg acgcgctttg tgctgtcctg gttgatgcgc cgggtgcgta
     5401 tttccagaaa acggtcgatg tcatcccggt aaaacgtgcg gatattgttt ttatcctcat
     5461 cccacccggt aatggttcgc ccgtatttcc ccgtgtcgtg atgccagttt ttcagcgtgc
     5521 gtgcttcgtt attccagata aagcgaatgc gttcctgtat ccgggttacg cggcgtctgc
     5581 gtggtgtcca cgcggtcccg tccggcgctt tctgtgaccg gatacgcgcc tgctgggcgc
     5641 ggcgtaaatc ctgtgccagc tttctggcga tgttattgat ggcctgctga ttcaggctgt
     5701 cgcggatggc ctcaaaggtt tcatccacgc gggtgaatgc cttatccatc gctttcaccc
     5761 cacgtcacat cctggaatac atgcgaccag tcgccttcgg gagatggcag gcggggtttt
     5821 ggctccggca ggtgttctgc ctgcggtgtc ccctgactgc tgcgcgtgat gcgaacgcgt
     5881 tcccgcaggg ggagcgtaaa caggagatcg gcgctgtcat cgtcattgat aacggcggag
     5941 aatttgatgt cctgattacg ctcagggttg agcaacaact gtggctgatt ttcggataac
     6001 cacgccagca gcggcagcgt gaggtcgtcc agctccccgg cgtaatccat gacaaacatc
     6061 accatctgat agcggtaaac aaacgatggg gtttctccgg tcgtttcaat gttgccgctc
     6121 tccacgaaaa tggtgaattt ttccgggttg gcctgacacc atcggcatga acgggtcatg
     6181 gcttcacgca gggaatcagt tttcagcatg gttgttatcc tcgttgttca gtcgttgcag
     6241 cctgcgctgt tccagtaatt caatggcccg tttatccgcg ttacaggttt ccagtgcatc
     6301 cagaaggcgg tcgccccata taccgagatt tccccatgtg ggagtatcag ggaagcgggg
     6361 aggcgttacc ggtatggtca gcgtctgcgg tataagccgg actgacggcg ctggcagtgg
     6421 cgcgttctgc gtgcctgcgc agcctgtcag taaaacgagc gtcaggcaaa gcgtgggcgc
     6481 attcatcttt tgcaatatcg ttgcgtagct gttcacgtct tacctctccg tcctgattgc
     6541 gttgctgatt ttccacgcgg agttgcgcca gcacctgctg catatcctgt cccccggcgc
     6601 tgatgatatt cagggtgtcg gcggtacttt tcagggtgct ggcctgtgct tcgtttctgg
     6661 cgttctcccg gcccagcgac caagacagac gcatggatgt tccccatccg gcaatcagaa
     6721 ggaaagcgac gccaagcgtg ggccagagct tcatgccgga taggctccgt gtggtaactg
     6781 aaaatgcggt ccgtctttca gggtcttcca gtcgccgccc cattccaccg gaatattcag
     6841 ttcccggctg gcctgtctga atgctgctgc gattttttcg tacagcggcc attcccatga
     6901 cacctggctg ccgatataag ccacaacatc cacggcatgc cccgtaaggt ggcggctgtt
     6961 catggtctgg ctcttacccg tggccaccag ttgcttctgg cggtaacggc tgcgcaaccc
     7021 ttcggtgata ccaaaatcca cttccgagat ttccagtgcc cgtcgggtca ctttcaccag
     7081 atcaggattt acgccctgca aattcttttc gctccggctg ctgaatttaa atgtgttgct
     7141 cattcgtcct tctccttcac cctgcgatta aaggccgcaa taaccttgtc gcgtgctttc
     7201 tctgcaccca taaaaccgat tgatgcgccg ataaacgtca cggcatcttc aggaaacccg
     7261 aagaagcgca acgacccggc cacggccatg gcaagaacgc cgcacgccag cgatcccgtt
     7321 acggtctgaa ccagtgttcg tccgtcataa agactcatca gcgcggaaat gctgaccgcc
     7381 gcgcctactg catacaccgt tggcaggtgg tcaaagagcc acgcaataac ctgctctgtg
     7441 atccctgttt gaatggtgct cactgctact ccccccacaa ctgaatcatt tctcgtttct
     7501 tcttctccgg ctccggcatc tcaacttcct gcccggcgtc cagaaatacc tgctgacaga
     7561 gtccggggtt ggcatccagc accttttcgg tgacgccctg cgtcgtgccg tagtaccgga
     7621 aacagagcga atccacggtg tcgccttcca gtgccttcac tttcatcagc acaactccgc
     7681 aaagattcgc gggcggcaca gaatgtcaga gatggcccag ctcacatcac gccacaaatc
     7741 cgatgtctgt atatccagag cgtccgcccg gcggtcgccc ttgtccgttg tgtctgcatc
     7801 acgataacgc tccagaatca gggcgcgcgt ggcggtataa accgcattgc gccagtgcca
     7861 gagattgacg ctttctccgt taattacagg ggccggaaca tcggccagcg tctgatggcc
     7921 agctgcctgc tgttcctgct gccatgcttc cagctcgcgg gtaacgtgtg ccacagcccc
     7981 ggtggcagta tgcagcaggc gggaggtggt cacgcggccc ggcagtcgta ccgccagacg
     8041 cagctcgcgc agcacaatat ccggccagaa tgcacccgct gaaatgcggg tatcgccatc
     8101 atcggtatcg gtgatgtcgt cctctgcggg tctgggttca gttctggcaa ccatactcat
     8161 ggggttcact cctgaaaaaa atcgggcggt gggtgcgcgg tgtaaacggt cacggagtca
     8221 aaccggaaca ccgcgcacgc cgcccgctga cggggtcagt cgttaaccgc gcttcgcctt
     8281 ctgcgtcgcg gtggtttttc gtgttgcagg cttccgcgtt gtctttttac ttttgctgct
     8341 ttcgtcctgc gcctgctgtg cgctggcgtc ttctggttcg gctgcggaat cggctttttt
     8401 cagggcgcgg gaaagggttg caatctcgcg tttcacacct gcgttcgggt tcaggtgcat
     8461 cgcttcgcgc agcagcttca gtgataaggc catgttgtct gcatcggtca gaccacgacg
     8521 ggcaaaggcg cacgccttgc ataatttggc gcgcacttcg tccggcatgt cctggtcggt
     8581 gacaatctcc cggagggtat ccagtggttc gataaaggcg gataaatccg cgtcggcatc
     8641 cgtcccggcc tgcgtcagta ccgggttgca gatttcttcg gtcagtaccg tggcagcagt
     8701 acggccaaag ttatccggca taatgaggtt atgacggacc acatacgcac caatacgcag
     8761 cgccagcgga agatcgccgc agtcaatcgc ccacaccatc agcgtggcaa tcacttcatc
     8821 ctgctgcccg ccgtcagcct ccagcgttcc ctcaatccag ccggaaaagt ccggcaataa
     8881 ctcttttttg atggcggctt tcgcgcttct ggcctgtacg cccttaaggc gggcctgtgc
     8941 cagacgcaga cgatacagca cctcttcatg cgcggtacgc gcggcgtggt ccacgccttc
     9001 attcgcccgg cctgcgcgct gtgccatcac gttctgccag tgttgctgtg caggagtaat
     9061 cattttttct ctccgttaca ggcgggcatg atgcccgccg tgagttgatt agctgtcggc
     9121 gaacttcagg ccagtgacca tcgcgcactt gccatagtct tcaacgacat aagcgtcatt
     9181 gatggactgg taggtggcga tgcggttgta ttccggttcg tctttcatca ggcgacgcat
     9241 tgaacctttc tgccagtaaa tcgacaggtt gttgaacgag gtgataagca tcgttgcatc
     9301 cgggaagaac ggcgcaagga acacgcccag cccgccaatg gtgcgcgatg acaggatgag
     9361 ctgcccggca agtaattccg cattgggatt ctggccgctg atgctgttca gcacgggcag
     9421 acgcagcgag ttaaacaggt tgcgccccat aatcaccacg aggtcgtcag cttccttgtg
     9481 ccattcatcc agcagggatg agcgtgcgtc ctgtaccagt gcatcagcgt tcgcatactt
     9541 acccgcgtgc gccacggtgt tgtccatgtt gcgggaggtc agcgtcacgt cattcataac
     9601 gcgttcgctg gcgtcggttc tgatgtgctc cagccagccc acgttaacgt cctgaagcag
     9661 cttgttggtg ctgaagttgg actcatccgc gtgagacgtg ccgttgaaac cgatcatgat
     9721 gcggtcaagc gccacctgtc gggcaatctg tgcgctgatg cgggactgaa aatcaggatg
     9781 tgccgcccag gcatcaagct gcggatatga aataaacgtg tcgtagttca cctgttcgca
     9841 ctggtatttg cggtttttca gatcaaccac gttattcggg ttacggcgtt ttgtgccgtc
     9901 ataactggta ttcgtgcgcg caatcggtcc tgtggtatcc aggaggattt tttcgccttt
     9961 ctggtcggtc acgccgatca cgttaattct ttttgtaaat tcggtgcttt cctttgaggc
    10021 gttttcaaaa cgctgctgca ccgagggttc tacggtaaat cgcgatacca gcgcagaaac
    10081 cgggatatta ttaagcgacg cctgctgcgc catatagcaa cccagcttgt tgcgggtaat
    10141 atctgacatc accagattca taaaaaattt gctcctttgt cttatcagaa gtcagccagc
    10201 tggtcggagg ctgcgcccgt tgcggtgaac cggttctgcg gatcgccgtc ctgcgtgcgc
    10261 agtttttcct tcagtgctgt cagctctgtg gtcagtgacg taattttctg gcggtcctgc
    10321 tgatggcggg tttccagcac attaaaacgg tcgataatgt cggcctgtga cgttgcgacg
    10381 ccttccaccg cttcctgaat acgggaaaaa ctggcgtcat ccgctttgcg gccacggcca
    10441 ataatcccca ttacgcggtt aaaccactgg gtgccttctt cctggcgttg ttctgccatt
    10501 tcgatgattt cagactcgat ggcttcggag ataagcggcg cttcaccctg gacactgttg
    10561 aacgtcatta ccgcctgacg ttgctgtgcc gtgaatttca ggcgctcagt gcccaggctt
    10621 gccggggtgt cggtcatcgc cagcccgacc agataggcac gcccgttaac ggagaactgc
    10681 gggtgcagtt cgatactgga atagattttc ttgccgtccg cgacaagctg cttcatgcgc
    10741 tcggtcggtt cgatttctgc atacagcgca gtacgtccgg ccagcggacc ttccgtaatg
    10801 tcttccgtac tcagtgcggt gacatcgccc attgcggaaa attcgcttga cgggcatggc
    10861 gagagatagt gctcaacgtt cacgcgggca gcgtaaacat ccgggttgaa gttctcggcg
    10921 gcttcacgca gatgcaccgg actgatttca cggccatcaa cagttgatcc ggagacagcc
    10981 acgcgaaact ttttgcggga tgtctttttt tcattagcca tagtttttgc ccctctgact
    11041 ggttcttcag tcatgatggc aaagcgtaac aggctgatac aaagggcttt tgttgtaaga
    11101 aaacggccag aacagggggt taaggagaac ggtttcgcgc gcgggtaatc ttcctgtaat
    11161 tactcagggg gagcaatgat tcaggacgct tttgtgcgcc agcgtgcgcg gcaactttac
    11221 tggcagggtt atccgcccgc agaaatatca cgtctgatgg gaataaaccc gaacacgatt
    11281 tatgcgtgga aaaaacgcga ccagtgggat gaaacgccac ccgtgcagcg tgtcacgcag
    11341 tccatcgatg cgcgcctcat ccagcttact gaaaaacaga ataaaacagg cggtgacttt
    11401 aaggaaatag acctgctgac ccggcagctt aaaaagctgc atgatggcca tccggatgtg
    11461 atggccgcag gaaagaaagg ccgggcgaaa aaactcaaaa atcatttcac gccggaacag
    11521 atttccgcac tgcgggaaaa aatcatcagc aggctggagt ggcatcagcg gggctggttt
    11581 gactccctga ccctttgcag ggaagccggg atacgtaaca ggatgatcct gaaatcccga
    11641 cagattgggg cgacctggta ttttgcacag gaagctctgc tgatggcgct gcgtgacgat
    11701 gtggcgcaac cttaccagcg taaccagatt tttttgtctg cgtcgcgtcg tcaggcgttc
    11761 cagtttaaaa gcattattca gaaggctgcg gctgaagttg atgtggagct gaaagggggc
    11821 gataaaatca tcctctccaa tggcgcagag ctgcattttc tcggcacttc tgctgcgtcg
    11881 gcgcagtcct atacgggcaa tttttatttt gatgaatttt tctgggtcag tcgctttgct
    11941 gaactgcgca aggtggctgg cgctatggca accctcagcg gactgcggcg cacctacttc
    12001 tccacgccat ccaccgaaac gcacgaagca tacgcctact ggaacggcga ccgctggaac
    12061 gagaaaaagg ccacgcataa acgccggcgt ttttctgtgg actggaatac gctgcataac
    12121 gggcttatct gccctgaccg gacgtggcgg caaattgtca cgctggaaga tgtggttaat
    12181 cacggctgga aacacaccga tatcgacgaa attcgtgatg aaaacaccga agacgagttc
    12241 ctcaatctct atatgtgtga gtttgtccgc gaaggggaat cggcatttaa cctgaatatc
    12301 ctgattggct gcggtgttga cggatacgac gactggaaag actggaaacc ttttgctccc
    12361 cgcccgatgg ggaatcgtcc ggtatggatt gggtatgacg caaacggcag cagtggcaac
    12421 ggcgacagcg gcgctgtgtc tgtggtggtt cctccggctg ttcctggtgg ccgttttcga
    12481 acggtggaga cgcgacgcgt tcaggggctg gagtttgaag aacaggccag agtcattgaa
    12541 gagttcacgt gtcgctacaa cgtggaacac atcggcattg atgtgacggg cgggaacggg
    12601 gaggctgttt atcagatagt gaaacggttt ttccctgctg ctattccgta caccttcacg
    12661 ctgtcatcaa aacggtcgct ggtactgaaa atgctgcaaa taatgcgtgc cgggcggtgg
    12721 gaatacgatc gcgccgaacg cgagctggtc gcggccttta acgccgtgcg taaggtgaaa
    12781 acaccgggcg gttttatcac ttacgaaacg gaccgcgcga gggggatcag ccacggcgac
    12841 cttgcgtggg caaccatgct tgctgtcatt aacgaaccaa ttggcggcga aggagaaaac
    12901 gagcgtttca cggttatgga gttctgatga gcagaaaaaa taaaaaagtg cgcatgagtt
    12961 cacgcattga tctcgctgat gcgctcagga aagaatcgtc gctcagtgca ttcacttttg
    13021 atggtcctta tcgcctgacc gggcatgatt tgctggacaa tatgtactgt gctgataacg
    13081 ggcggtggta tgaaaccccg gtggactggt acggtctggc aagagccgcc cggcaaacgt
    13141 cctggcatca gtctgcgctt tactttaagc gcaatgtatt gctcggctgc tatattccgc
    13201 acccgctgct ttcccggcag gatttctcgg cgctggcgct ggactggttt gtgttcggta
    13261 acgcatacct tgagcttcga agcaatatgc tcggcgaacc gcttaaatta cggcacgcac
    13321 tggcgaaata catgcgacgc ggaagcgatc ttgaatcatg gtggtatgtg caggatggca
    13381 aggacgcgtt tcagtttcgc cctggcaaag tgtgccacct gatgaatccg gatattaacc
    13441 aggaaatcta cggcatgccg gaatatctcg gcgcattact ctcggccagc ctgtctcatt
    13501 cggcggacat gttcagaaaa ctgtattacg acaacggatc ccacgccggg tgcatcatct
    13561 acatcggtgc agcgcaggta aaccgcgaaa gcatggactc cctgaaagaa acattacagg
    13621 gtgcacgtgg tagtggtgca tttaaaaacg tgctcattca tgcgcccaac gggggcaaag
    13681 agggggtgca aattttgccg ttccagcaga tcaccgcaaa agatgagttc atgaatgtta
    13741 aggcggcatc ccgtgatgat gtgctggctg cgcaccgtgt tccgccgcaa ctgatggggg
    13801 cgatgccggg cgaaaaaagt gcgtttggtg atgtggagaa ggccgcgcgg gtttatgcaa
    13861 ttaacgagct gatgcccgtt atggaggcca tgaagcacat caatgactgg cttggcgaag
    13921 aggtgatccg ttttaattct tatgctcttc ttgatgaaaa aacagccccg tgatggggct
    13981 gtccttttta ccagagttga accattttct gggtgccgtc aggcttgaga ttatcaattt
    14041 cagagagaac gtaatattga atggcttcac aaacggtggt gtagggtgaa ttaccttctt
    14101 taagtggcac gatattatta ttaacgcgaa cctgtatttc atcgttatac attgcgatcg
    14161 aaagaggagt atgcacgaag gcgacttcgc caggtgtgtc gtcaaccact gtctcaatgc
    14221 tgaaaatcag ttttcgctca tcattgccgc cccttgcttt gggggtagag gcaggaatct
    14281 gagataaagg cattctgcga aacccttcag ttgtttccag tccgcatgaa acgtaatgct
    14341 ggcgattacc gtcgctatca gtccatgtct gtgatggcag ctccagagag atttcataag
    14401 catctacaat tccctgagca aggtgtacaa gcggggtcag atcttcattg cggcgaaaac
    14461 tttcctttac ctgctctcgt ttttctcgta actgcttgta attaatgacc ataaaacagc
    14521 ctccattgat ttctttgctc gtattttgca cttatgaagt gtggtcggca aggtgccgca
    14581 tcactgacgc gcttcgcttg tctgctgctt cgccggggca taaaaaattt atgccccggc
    14641 tctccagctc ctgtatcaat cagataattt cacgacgcct tccagtttat cgccaccatc
    14701 gacggtcaga ctcttacgca atcccaccgc gttgactgta tgttctcgct gcctcagtgc
    14761 gattttgacg gccttacctt tcaccccatc aaatcaaaag ccctcacgcc tttttcatgc
    14821 tcagcgtgag aaatatggcc attctgttgt gtctctgcga catcgttcag ggaatgctgt
    14881 ttacccccct gaaacgcggg ctgttccccc gtcacctgcg cgtagaaaaa acgcgttttt
    14941 tgtgcacgta cggatccttg acggatccag ccgccacgcg ggccggaagt acaaaaagtc
    15001 gttcaaaaaa attgtgcaaa cttgtgcact atcgtgcaaa caaaaaaaag cgccttatcg
    15061 gcgcttcaaa aatatcaatt gttgctgtgt attaatcgcc aaccacgaac atatgcttca
    15121 taagcatctc tgtgccttac agttccagcc tggctaaacg gaatgttagc taaaactaaa
    15181 tcattctgag ccatagcgcg tccttcaagc gcatctttta tgcctagctc aaaagcacag
    15241 gcagcgcatt tgtgccgacc ttcttgtcct tgatattcag ggagagacag aaatgttggg
    15301 ttataacgat gagggttctt gcaaatacct gttttagccc gcactttatt tacctcatag
    15361 gaaaaatatg cgtgccttta cagaggtgcg tgtaagcaat aaaatatacg acgcacattt
    15421 tgatttactt cggaaggcac gcatatcagg ttaagtgagt ccgaccgagt tctacgcccc
    15481 aatagttgct gctattgggg cgttttgcat ggacaatgcc gcgcaattat cttgtcgctc
    15541 acaatgcgaa cgatcttaca aaaaggcaca ttactgtcaa gataattgat cgttttaatc
    15601 gatagataat agacaatcta tttgtttaac agatcgatta ttgaagtaag tgcgccaaat
    15661 ggaatgatac tgtttctata aatgtgagca tttttcgcgc agatgctttt actcaggaaa
    15721 taacgcccgg atattcccag ccatctggct ggttatctta gccactggtg cagactgtgc
    15781 ttcaaacttt tttgagctga tttgtgtcac aggtaacatc tcatcatcag cccatgcggc
    15841 cagtcggtaa gcctctgccg gattcgtctt cagaagtgcc agcccggcca gaaaagccac
    15901 gcgttggccg cttttgcggg cttctggtgt aaggctgtcc agccaggcgc atgcttctcc
    15961 ttcgttcttg acggcagctg gcttcagata gaaacttatt cttctggttg gtgtcgtcat
    16021 tggtttactc cttgttcatt gcgtacagcc cattaaccag agcaaaatgt ggcaccccgt
    16081 ccgtgatgaa agtcgcatta actccgcagg cttcgcggat agcgggtgcc acaatctccg
    16141 cccctccacc gacaaccatc acccgcccgt aaccggaaaa acccgccagc gcgcggatca
    16201 ctcgttgttt cagtgtctcc tccttttcac gaataaccgc catcaggctg ttgtaatgcg
    16261 cgtcattgtg aatgtgctgg cgcagccagg cttcatcgtg acggtgctca ataatggtat
    16321 tggcgatgtg gtgactggta cgcataccgt tagtggccat caccgacagc acggcatcag
    16381 ccatcaggga aacgcctacg tgtggatcgc aaaacacctg gctgatacct gccagctgcc
    16441 cctgaacctt tgccacatcc agcgtggttc cgcccaaatc cacaatcagc agggattcaa
    16501 acggactcat gtcagccagt gccttaaagc cagccggaat ggattcaggc ataacccgca
    16561 cgttacggat agtgaatgct tcgccgttct ggtactccac cgggcgcata acgttcgctt
    16621 ttttgcggtt gatgttggct atgtccggct gtgcgtttgt gtcgaaatac tcgctcagtg
    16681 gcagggtgac aaccacatcc acctcctgtg gcgtgatgcc tgatttgacc agcgcgtgat
    16741 gaatggcaat gacattcaca tcactgtatt ggtattgcgt gtcggtcgtc tggacaaagc
    16801 gatcgctgac cggatcaaaa ccatagcgca cgccatcaag catgtagttc gcgggctgcg
    16861 tgccaccgaa cggcgcagac cattccgact tgaagctgtt cgggctgatg gcgttgcggc
    16921 gttcgccgtt ctcagtccat gccagcttga tgttggtgga gccgtcgtcg atacaaattt
    16981 tcatgtcgct tttccttatg ttgattaatt aatcgtttac gggattctga aatcccgttt
    17041 ttgcctgttt tgtgcgcgct tcatatatcg ctgcgcgttt tttgctcatt tacgggattc
    17101 gtgagtcccg tttctgtctg ttttttgttt ccactggtca ggccaccctg cagcaggtct
    17161 gctttgcggc tggcgcgttc agtggtttca ctgattctct gtgcgtgctc tgcgtcgcgg
    17221 atggcgcgca gcatgtcaga aagcacggta acgggtgttt tcatggtgtt ctgatcctgc
    17281 tgaagtgcgg atgccaggcg tgcggcggct tcggggtctg atgcccccag ctgtgccaga
    17341 tagctggcga ccgggttatg gcggatctcc gtgctgctta cgccgtgatt acggctcagg
    17401 tgctgccaga gctgcgtgat tcggctgtcc gggcgggtat ccggtttgcg tacaatttca
    17461 tatccctgcg gtgcaatgat gctgccgtca acgtacaggc tgccgccccg tagcaggtgc
    17521 tgcatctgct gttcaccgat atgcaggccg agagattcgg cagactcccg ccattcttta
    17581 gcgagtaatt cgtggttatc aggcaaaggc cgctgctgtt tgcggctctg tgtccagctc
    17641 tgcatttcat cactgctgtt ttttgcctgt ttgtcacgaa gcgaacgcat cagcgcccgg
    17701 cgttcgtgcc gtttcagtga gcgcatccat tcgttcactt caacgccgtc agggagctgc
    17761 ggccacggtg ctggccgttc ttccggctgt tctgccccgt tgttgtccgt ttcctgtaca
    17821 cggggacagt tattgccacg agtccaaggg gcggcagggc cgccctgaag gtcaaaacca
    17881 ttttcgcggg cgctgtcttc cgtttccggt ttacgtctta ccagtttcca gttatccgga
    17941 tgcgtgcaca cacgggagga ttcccctatg agtggtgacc agatcccgta aatctgtacg
    18001 ctctgttcgc cgtaatcatt cagctcatct gcgaggtcgt aggcggtgcg aatcaggtag
    18061 tctttgcgtg gaacaagtac gccgccctgt ttttcaatgt aggtggcaaa acacccggca
    18121 tcggcggcag cgagtaccgc atccattgca tcatctttca gccgttgcgg gccttccgga
    18181 ttgcgtgcca tctggctggc aaggcggcgc agttcacgcc acacctgacg ggaggggatg
    18241 ccaaagaact ggaactgacg gacccggtga aggcgcgccc agccgatggc gcgctccacg
    18301 ctctcggcca ttgattttcc ggtttcgtgg tcaacgcgtg gcttgcccgt tttcgggtcg
    18361 atgccatcca cggcgcggct gtccaggttc tttccgatgt aggtggcgat gtagctggtt
    18421 ggtgtgcctt ttgagccgtc gacatactcc gccttaaaac gcggagttat gtcatcgccc
    18481 agctcgtggc ggtcctcc
//
LOCUS       DAZUFB010000095        18252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZUFB010000095 DAZUFB010000000
VERSION     DAZUFB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.095"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..238)
                     /locus_tag="ACHY3M_003692"
     CDS             complement(26..238)
                     /locus_tag="ACHY3M_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318524.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            complement(344..766)
                     /locus_tag="ACHY3M_003693"
     CDS             complement(344..766)
                     /locus_tag="ACHY3M_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS4318525.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
     gene            complement(782..1543)
                     /locus_tag="ACHY3M_003694"
     CDS             complement(782..1543)
                     /locus_tag="ACHY3M_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4318526.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPAELPEETTMATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PTGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIFDSSSRIVSEK"
     gene            complement(1566..2312)
                     /gene="ydaV"
                     /locus_tag="ACHY3M_003695"
     CDS             complement(1566..2312)
                     /gene="ydaV"
                     /locus_tag="ACHY3M_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4318527.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELVTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEK"
     gene            complement(2319..3107)
                     /locus_tag="ACHY3M_003696"
     CDS             complement(2319..3107)
                     /locus_tag="ACHY3M_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318528.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQINTVSFPDSAIPPGFRG"
     gene            complement(3185..3607)
                     /gene="ydaT"
                     /locus_tag="ACHY3M_003697"
     CDS             complement(3185..3607)
                     /gene="ydaT"
                     /locus_tag="ACHY3M_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4318529.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(3604..3858)
                     /locus_tag="ACHY3M_003698"
     CDS             complement(3604..3858)
                     /locus_tag="ACHY3M_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4318530.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            3938..4357
                     /locus_tag="ACHY3M_003699"
     CDS             3938..4357
                     /locus_tag="ACHY3M_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4318531.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            4600..4779
                     /locus_tag="ACHY3M_003700"
     CDS             4600..4779
                     /locus_tag="ACHY3M_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318532.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            4790..4945
                     /gene="ydaF"
                     /locus_tag="ACHY3M_003701"
     CDS             4790..4945
                     /gene="ydaF"
                     /locus_tag="ACHY3M_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HFS4318533.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(4942..5430)
                     /gene="sieB"
                     /locus_tag="ACHY3M_003702"
     CDS             complement(4942..5430)
                     /gene="sieB"
                     /locus_tag="ACHY3M_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFS4318534.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            5872..6093
                     /gene="kilR"
                     /locus_tag="ACHY3M_003703"
     CDS             5872..6093
                     /gene="kilR"
                     /locus_tag="ACHY3M_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFS4318535.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            6093..6263
                     /gene="ydaE"
                     /locus_tag="ACHY3M_003704"
     CDS             6093..6263
                     /gene="ydaE"
                     /locus_tag="ACHY3M_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFS4318536.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6338..6613
                     /locus_tag="ACHY3M_003705"
     CDS             6338..6613
                     /locus_tag="ACHY3M_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318537.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            6715..9315
                     /gene="recE"
                     /locus_tag="ACHY3M_003706"
     CDS             6715..9315
                     /gene="recE"
                     /locus_tag="ACHY3M_003706"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFS4318538.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            9308..10117
                     /gene="recT"
                     /locus_tag="ACHY3M_003707"
     CDS             9308..10117
                     /gene="recT"
                     /locus_tag="ACHY3M_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFS4318539.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            10173..10322
                     /gene="ralR"
                     /locus_tag="ACHY3M_003708"
     CDS             10173..10322
                     /gene="ralR"
                     /locus_tag="ACHY3M_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFS4318540.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            10360..10548
                     /locus_tag="ACHY3M_003709"
     CDS             10360..10548
                     /locus_tag="ACHY3M_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS4318541.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            10648..10863
                     /gene="xisR"
                     /locus_tag="ACHY3M_003710"
     CDS             10648..10863
                     /gene="xisR"
                     /locus_tag="ACHY3M_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFS4318542.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            10865..12100
                     /gene="intR"
                     /locus_tag="ACHY3M_003711"
     CDS             10865..12100
                     /gene="intR"
                     /locus_tag="ACHY3M_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4318543.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            12152..13087
                     /gene="ttcA"
                     /locus_tag="ACHY3M_003712"
     CDS             12152..13087
                     /gene="ttcA"
                     /locus_tag="ACHY3M_003712"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFS4318544.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(13216..14589)
                     /gene="dbpA"
                     /locus_tag="ACHY3M_003713"
     CDS             complement(13216..14589)
                     /gene="dbpA"
                     /locus_tag="ACHY3M_003713"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFS4318545.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(14619..14792)
                     /gene="ynaL"
                     /locus_tag="ACHY3M_003714"
     CDS             complement(14619..14792)
                     /gene="ynaL"
                     /locus_tag="ACHY3M_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFS4318546.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(15067..16050)
                     /gene="zntB"
                     /locus_tag="ACHY3M_003715"
     CDS             complement(15067..16050)
                     /gene="zntB"
                     /locus_tag="ACHY3M_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFS4318547.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(16064..16111)
                     /gene="ydaN"
                     /locus_tag="ACHY3M_003716"
     CDS             complement(16064..16111)
                     /gene="ydaN"
                     /locus_tag="ACHY3M_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFS4318548.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            16305..17537
                     /gene="dgcM"
                     /locus_tag="ACHY3M_003717"
     CDS             16305..17537
                     /gene="dgcM"
                     /locus_tag="ACHY3M_003717"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFS4318549.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(17558..18121)
                     /gene="smrA"
                     /locus_tag="ACHY3M_003718"
     CDS             complement(17558..18121)
                     /gene="smrA"
                     /locus_tag="ACHY3M_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFS4318550.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         5061 a         4400 c         4253 g         4538 t
ORIGIN      
        1 cgccctttcg ggcggcctcc tgacattaat cgttgtgata actcatagct tcatttgcag
       61 catcaactgg atcaacctcc ctccagcaat aatttgggtc ggctccttca ggcgtccacg
      121 gttctaattc attttttgcc gcattctcat cgccagtaat tttaaaaatc tgctcagaga
      181 attttcttgc ccactcgtta tatttttccg cattaatggc tttctgtgta tttaacataa
      241 atatacctcc agttaaggat taaattttat ttacagtgct gaacttaatt attcagattt
      301 ggattatgct ttctcttcac gaagttccga ttgttaattt ggctcacaac agcaccttct
      361 gaaaattacc ctgatagaaa gccagtacac gctgcatagc ttcgctcttc cggcactcgc
      421 tacagattat gttcagacgc ctgtcgtagc ggcgtatttc tccgtctggt aatgaccaga
      481 taagatccgg atcaaccaca accggtttct tcacctttgc ccttgatagt tttttgcggg
      541 cgttttgcca gtctttacgc gcctgctcag acgggaataa tccgtagcct gaattgtaaa
      601 catcaccact ggcgaccagt tctctggcga gagtgcttat gtaatacctt gatgcaccgg
      661 ttttagcctc cagagcccgt aacgtctcgc gaccgctcag acgtacaagt tcaacaacct
      721 gccctttaat tttttcccgc tcttctggtg taaatacttt tgccataggt gcctccggca
      781 atcacttttc cgacacaata cgactggagg aatcgaaaat ctgtcgaaca atatcccggt
      841 gcttgttcag ctcccgcagc gcggcgcaga ctcgctccca cttctggaca tgatttttcg
      901 cccgacgcag ttcgcggttt gccatatgca gcgatggtaa aaccaggtca tccgctcgcg
      961 tttcagtaaa cgatggcagc gactgcacaa tgtccgccac cgtttctgtt ttaatatctt
     1021 cctgtgttgc agcctcctgt actggtaacg caacacctgt gggctgagga aaggccttac
     1081 catcagtttc cgctaccgat gctgctttcg gctctgctgg taaattatcg cccggtatgc
     1141 agtaacgaaa tttaccgttc tggtttacgc gaatcaggcg tcctttgctg atagccattg
     1201 ccagcgttga agctactttg cgtgatgtgg taccgaacaa tgtagccaac tcatccgccg
     1261 tttgtgggcc acgttgttca atcgtcgcgg ttaaatcgca ctctgagatt ttcgctactg
     1321 ttgccatggt ggtttcttcc ggcagctctg ccggcgctgg ctgttcctgc tgaacgttgt
     1381 tatcagccac gcgccaggtg tatacgcttt tatcaacgaa gccagccttt ttcagttccc
     1441 acagctcgtt cagcacttct tctcgactga tatcaagtcg cgtagccagt tctaccgacg
     1501 tggcttttcc catcgctttc agtgcgtcaa aaacggtctc cattaaaatt tcctcccggt
     1561 aaaaattact tctcaatacc tggctgacca acattcgggc gccagctctc ccagttaaaa
     1621 ttcacccagc gcccgccgtt catggtcatg cgatccataa tccgctcgcc gagcaatgtt
     1681 ttcatggcct catagttcag gtttgtcagc atccccacgc tgcgcattga cgctgtccgg
     1741 cgatcaacaa tctggtgcag taccacctgc tcgtttttcg tctcgcgctg aataccaatt
     1801 tcatcaagaa ccagcagatc aacttcgcac agttcccgca aaaatttttc gcctgactgc
     1861 ccgtcgtcat agctggcgtg cagggcgctc ataacatcag ccacggtaac cacaatcact
     1921 gtctggccat ctttcagcag gcgattcccg atagccgccg ccagatggtt ttttccggta
     1981 ccaggttttc cgctgaacgc gaaatttgtg catccggtaa ccagttcgtc agcgatagat
     2041 ttcgcctgac tcaacgcgta tcgctggccg tcgttctgca cctggtaatt cgcaaacgag
     2101 catttgcggt gcaacggctg tatgccggag cgattcagga ttttttccac ccgcaactga
     2161 cgattctgac ggttgatctc ctcacaacgt ttctggcctt ctgcaagttg ccactcgcgc
     2221 cactccgcca ccgtcctgaa tggcgcggtt acatgtggcg gggccagtct gcggatacgt
     2281 tccagaacgc cgcctgtcgc aatatttttc atggtctgtt accccctgaa gcctggcggg
     2341 atcgcactgt ccggaaacga gacggtgtta atctgtcgga gcaacgtctc tggccgaaca
     2401 cctttcggcg cgaacaagcc ctggtattca ttggcgatgc tgtgtcgaat cacctgctca
     2461 ggtgaaaaac cctgctggcg gaatttttcc agctcccgta tcgccccgtt agcgccctgc
     2521 tccgttcgaa tcggttttcg caatgcctgc ctgaactgaa cccactcatg ccagagtgtt
     2581 tccggcaacc agtcaggcag ctcgatagcc tccggttcga attttttaga cgctcgtttt
     2641 tggcgagggg gatttagggg gagataagta tttatatctt cctctttctc ttcctctggt
     2701 aacgcttttt gatccgtttt tgtaacgctg gcagcgttac cttttcgttt cagttctcgt
     2761 atttttgtta ctctctcgtt tgtaaccgcc cgttttttag agcttttccc gttatggcgc
     2821 tcaaagttag gaagcgacaa cacaccatta gtttcgacca gccatccaac ctgaattaac
     2881 gcatcagcaa aaccagccat aaaagtgatg cgatctattg cactttttgt aacgccgcga
     2941 gcgttacact ctgcgttacc gtctatcatt tgttgatccg cccatgccca gaagcgaatg
     3001 actttcccta atgcggcatc tggatcaata ttcagaatct cagcaagcct gaatatttcc
     3061 ggcttatccg gcgtaataac ttcgagcttt atccagtttg aagccatttg ttttcacctt
     3121 gtaacgctcg cagcgttaca tttaactgat accgaacaaa acagttcggt acgattaatt
     3181 tcaatcaatg cactacgaca gaatcgctag gagaaccgcc gccgctgaaa tgtgctttac
     3241 ggtaaacggc ctggactgca tcatcatgcg catcaattgc cgtactcagt gcatcctgtg
     3301 ccgccagtaa tgcacggcgt tccagggtat cgaagatgca gagtcggtga cgcagctcgc
     3361 gaggaagaat tgccagaacc gcagggatca gtttctgaat tttttccctt tgcgcattcg
     3421 tttcaccttt caaccaacga tgatagatat tctgctgatt gttccagtcc ttgcctggta
     3481 ccaggggcaa ttcgccgccc ccctggcgca gatattcttc agtaattgcg ttagcgaccc
     3541 acgcctgccc tttttcggct gccagggcta acaacactga ttcgatgtgc tcatgcttga
     3601 ttttcatgaa tcaactccca tcagcttttt cgtagtagtt ttatttctgc caatagttaa
     3661 aattgcatcg gcagaaaata atccgtttga tgcatgagcg attttttcag cgtaatttgt
     3721 ttcgccggta tattctgtgc gaggcaattt accgttatcc atccatttgt agattgctct
     3781 ttggctgaca ccacaaacgt cggccacaac agaaacgcga acagttttga ttacatcttc
     3841 aagtgttttc tggttcatat caccctcaca atgtgaactt tgagtacatg ctataacaga
     3901 actgacagta cattcaagag cgaatatcat tgaacttatg gttcatgaag ataaagcgcg
     3961 taaagagttc gccagtaggc ttgcgctagc ctgtgaaaac gctggttatg aacaacatgg
     4021 aaggcaggca gaaattgccc gtcgaatgaa attaacacca aaagcggtta gcaaatggtt
     4081 taatggtgaa acaattcctc gccgagagaa attaagggaa ttagcaacac tcattggaac
     4141 aacaccaacc tatcttttgg gagaggatac agaagaaagt ggacagatac gtttctatca
     4201 ggagttaaat ccaagacaaa aaatcatcat tgaccttctg gacgagctcc ctgacagtga
     4261 gacagatgaa cttttaaaaa ctcttgagga gaaaaaacag aagtacaatg caatttacga
     4321 agagttagca cgaaagaaaa aacaaaaagc ctcttaaacc agcataaatc cggtagcgtc
     4381 cccctccggg tttgtgcttc actttatccc gtctcatttt tttatacata aaatgtactt
     4441 aaagtacttt acaatgatga acacaaagta cattatatac ctaccaaccc accccgcccc
     4501 acagaacgcc gggcaatact tcgagttacc aggcagtggt caggggttaa gtagccagcc
     4561 cgaggcgtat gaacatgacg gcgggattca aattttgcag tgcagcagtt agttccgcca
     4621 cccggcgtta aggggataga taagatggtg cattacgaag tagttcagta tttgatggat
     4681 tgttgcggta tcacttacaa ccaggctgtg caggctttac gcagcaacga ctgggatctc
     4741 tggcaggcag aagtcgctat acgtagcaac aagatgtgag attcgcaaaa tgcaaaaaat
     4801 cgacctcggc aacaacgaat ccctggtgtg cggcgtgttc cccaaccagg atggaacgtt
     4861 cactgccatg acgtatacca aaagcaaaac atttaaaacc gaaactggtg cgcgccgatg
     4921 gttggagaag cacacagtaa gctaacgatt aaaacgtcta ctcctgctgt tccagaataa
     4981 cttcataaaa tgggagtatt tttcggtgac gagataataa gaacagtttg cgctatcact
     5041 ctgatgttga atgatgccct tccgttctaa ttttttcata accgggttac ggcaaggaga
     5101 agtgataata agatttcctg ttttaaggaa atctttaaat acagcgattt ctttctcaga
     5161 taaacgaagc aatactcgtt gctctggtag taatgaataa tgcttttgaa tatgtgctcg
     5221 caatcttgag aaggaaatgg cgaccacgaa agaaaaggca aaaacgataa tctgaaagag
     5281 ccaaggtatt tcagtataag cattgaatgc gacagtaaac tctttcggta tcagccagag
     5341 agtgagacca aaaatgataa tcgtatacat aagtctttcg agtggctcgt tagcaaaaag
     5401 tttcaacaat ggagtaaata catccaacat atcaataact ctcaactgta agggtattga
     5461 aatgttaaca caagctctcg ctgtaggggt atagccgaga ccaccgaagc ccggaggtgg
     5521 tgaaataaaa ccgggcacaa cacgaaggcg catttccgat atccataaag agtcggtctt
     5581 gtctgttaaa tttaaatggt gggagtgcgc ctccggttgt aaataacgac attgctgtgt
     5641 gtagtcctgg cggcatcagt ttttttcttg aagttcggct gatgtccgcc ctttttaaag
     5701 tgaattttgt gatgcggtga atgcggctaa gcgcacgtgg cacagttaaa agtcatgtta
     5761 gtccttattg gtttgggtgg gaaagccgac tgtaattgtt aactggttgc agtcacctgg
     5821 aggcaccagg caccgcatca acaaagttca tttgtaaaaa tggagataat tatgattgca
     5881 catcacttcg gaactgatga aataccacgt cagtgtgtga ctcctggcga ttatgttctt
     5941 catgaaggcc ggacatatat tgcctcggca aacaatatta aaaagcgaaa actatatatt
     6001 cgtaacctga ccacaaaaac atgcattact gaccgcatga ttaaagtctt cctcggtcgt
     6061 gatggtttac ctgtaaaggc ggagtcatgg tgatgactaa gaaaataaaa tgtgcttacc
     6121 acctttgcaa aaaagacgtt gaagaaagca aagctattga aagaatgctt cacttcatgc
     6181 acgggatttt atcaaaagac gaaccgagaa aatattgcag tgaagcttgt gccgaaaaag
     6241 accagatggc acatgaactt taattaattg actattcgaa actgaattta tgccagaaat
     6301 ggcaggtatt cgctcaacct taattaagga gaaaaacatg attaccaatt atgaagccac
     6361 tgttgtaact accgatgaca ttgttcacga ggtgaatctg gaaggaaagc gcattggcta
     6421 cgtaattaaa acagaaaata aagaaacccc attcactgtg gttgatatcg atggtccatc
     6481 aggcaacgta aaaacacttg atgaaggtgt caaaaaaatg tgcctggtgc atatcggaaa
     6541 gaatctgccc acagaaaaaa aagccgaatt tctggcaact ctaattgcaa tgaaattaaa
     6601 aggtgaaatc tgaaagaaat agcctgcgta tggcgcaggc tatgaacagt gtgtatccgg
     6661 caagatcatt cactgaacaa aacgaatttt aatctgagtt gaggttaaaa aacaatgagc
     6721 acaaaaccac tcttcctgtt acggaaagcg aaaaaatcat ccggtgaacc tgacgtcgtc
     6781 ctgtgggcaa gcaacgattt tgaatcgacc tgtgccactc tggactacct gatcgttaag
     6841 tcaggtaaaa aactgagcag ctattttaaa gctgttgcca cgaattttcc tgtcgttaat
     6901 gacctgcccg ctgaaggtga gatcgatttt acctggagtg aacgctatca actcagcaaa
     6961 gactccatga catgggaact aaaaccggga gcagcaccag acaacgctca ctatcaaggc
     7021 aataccaacg tcaacggcga agacatgact gagattgagg agaatatgct actcccaatt
     7081 tctggccagg aactgcccat tcgttggctt gctcaacacg gcagcgaaaa accggtaacg
     7141 cacgtttcac gcgacggact ccaggcatta cacattgctc gggctgaaga actaccggct
     7201 gttactgccc tggctgtttc ccacaaaacc agcctgctcg acccgctgga aattcgcgaa
     7261 ctccacaaac tggttcgtga cactgacaaa gttttcccta atcctggtaa ttcaaacctg
     7321 ggactgataa ctgctttttt cgaagcatac ctgaacgctg actacaccga tcgaggactg
     7381 ctgacaaaag agtggatgaa gggtaatcgt gtttcacaca tcactcgcac ggcttccggt
     7441 gctaatgctg gcggcggaaa cctcaccgat cgcggcgaag gtttcgtaca cgatctgacg
     7501 tcactggcgc gcgacgtagc cactggcgta ctggcccgtt caatggatct ggacatctat
     7561 aaccttcatc cggcacacgc taaacgcatt gaggaaatta tcgctgaaaa taaaccgccc
     7621 ttttctgttt tccgcgacaa attcatcacc atgcctggcg ggctggatta ttcccgcgcc
     7681 atcgtggttg cgtccgtaaa agaagcacca attgggatcg aggtcatccc cgcgcacgtc
     7741 actgaatatc tgaacaaagt actgactgaa accgatcatg ccaaccctga tccggaaatc
     7801 gtggatattg cctgcggtcg ctcctctgcc ccgatgccgc agcgagtaac agaagaagga
     7861 aaacaggatg atgaagaaaa accgcaacca tctggaacaa cggcagttga acagggagag
     7921 gctgaaacaa tggaaccgga cgcaactgaa catcatcagg acacgcagcc gctggatgct
     7981 cagtcacagg taaattctgt tgatgcgaaa tatcaggaac tgcgggcaga actccatgaa
     8041 gcccggaaaa acattccatc aaaaaatcct gtcgatgccg ataaattgct tgctgcatca
     8101 cgtggtgaat ttgttgacgg aattagcgac ccgaacgatc cgaaatgggt aaaggggatc
     8161 cagactcgcg attgtgtgta ccagaaccag ccagaaacgg aaaaaaccag cccggatatg
     8221 aatcaacctg agccagtagt gcaacaggaa ccggaaatag cctgcaatgc ctgcggccag
     8281 actggcgggg ataactgccc tgactgtggt gcggtgatgg gcgacgcaac ataccaggaa
     8341 acattcgatg aagagagtca ggttgaagct aaggaaaatg atccggagga aatggaaggc
     8401 gctgaacatc cgcacaatga gaatgctggc agcgatccgc atcgcgattg cagtgatgaa
     8461 actggcgaag tcgcagatcc cgtaatcgta gaagacatag agccaggtat ttattacgga
     8521 atttcgaatg agaattacca cgcgggtccc ggtgtcagta agtctcagct cgatgacatt
     8581 gctgatactc cggcactgta tttgtggcgt aaaaatgccc ccgtggacac cacaaagaca
     8641 aaaacgctcg atttaggaac cgctttccac tgccgggtac ttgagccgga agaattcagt
     8701 aaccgcttta tcgtagcacc tgaatttaac cgccggacaa actccggaaa agaagaagag
     8761 aaagcgtttc tgagggaatg cgcaagcaca ggaaaaacgg ttatcactgc cgaagaaggc
     8821 cggaaaattg aactcatgta tcagagcgtt atggctttgc cgctggggca atggcttgtt
     8881 gaaagcgccg gacacgctga atcatcaatt tactgggaag atcctgaaac agcaattttg
     8941 tgtcggtgcc gtccggacaa aattatccct gaatttcact ggatcatgga cgtgaaaact
     9001 acggcggata ttcaacgatt caaaaccgct tattacgact accgctatca cgttcaggat
     9061 gcattctaca gtgacggtta tgaagcacag tttggagtgc agccaacttt cgtttttctg
     9121 gttgccagca caactattga atgcggacgt tatccggttg aaattttcat gatgggcgaa
     9181 gaagcaaaac tggcaggtca gctggaatat caccgcaatc tgcgaaccct ggctgactgc
     9241 ctcaataccg atgaatggcc agctattaag acgttatcac tgccccgctg ggctaaggaa
     9301 tatgcaaatg actaagcaac caccaatcgc aaaagccgat ctgcaaaaaa ctcagggaaa
     9361 ccgtgcacca gcagcaatta aaaataacga cgtgattagt tttattaacc agccatcaat
     9421 gaaagagcaa ctggcagcag ctcttccacg ccatatgacg gctgaacgta tgatccgtat
     9481 cgccaccaca gaaattcgta aagttccggc gttaggaaac tgtgacacta tgagttttgt
     9541 cagtgcaatc gtacagtgtt cacagctcgg acttgagccc ggtagcgccc tcggtcatgc
     9601 atatttactg ccttttggta ataaaaacga aaagagcggt aaaaaaaacg ttcagctaat
     9661 cattggctat cgcggcatga ttgatctggc tcgccgttca ggtcaaatcg ccagcttgtc
     9721 agcccgtgtt gtccgtgaag gtgacgagtt taatttcgaa tttggccttg atgaaaagtt
     9781 aatacaccgc ccaggagaaa acgaagatgc ccctgttacc cacgtctatg ctgtcgcaag
     9841 actgaaagac ggaggtactc agtttgaagt tatgacgcgc aaacagattg agctggtgcg
     9901 cagccagagt aaagctggta ataacgggcc gtgggtaact cactgggaag aaatggcaaa
     9961 gaaaacggct attcgtcgcc tgttcaaata tctgcccgta tcaattgaga tccagcgtgc
    10021 agtatcaatg gatgaaaagg aaccactgac aatcgatcct gcagattcct ctgtattaac
    10081 cggggaatac agtgtaatcg ataattcaga ggaataattc agcctggcgg tgtaatgcac
    10141 cgccaacttg aaatattttt atgagaaaaa ttatgagata tgacaatgtt aaaccatgtc
    10201 cattttgtgg ttgtccatca gtaacggtga aagccatttc aggatattac cgagcgaagt
    10261 gtaacggatg cgaatcccga accggttatg gtggaagtga aaaagaagca ctcgaacgat
    10321 gaaataaacg aaccactgga aataataatg gaggtgttca tgtataaaat taccgccact
    10381 attgaaaagg aaggtggcac tcctactaac tggacaagat attcaaaatc taaactaacg
    10441 aaatcagaat gcgaaaaaat gctctcaggt aaaaaagaag caggcgtttc cagagagcag
    10501 aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat tgcattgtat
    10561 tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa tttaagtcta
    10621 acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga agagtggatg
    10681 gttgaatacg gcctgatgct tcgcactggt ctgggggcca gacaaattga agcataccgc
    10741 cagaactgtt gggtggaagg cttccacttc aaacgagtat ctcctttagg gaagccagac
    10801 agtaagcgag ggattatctg gtataactat ccaaagataa atcagtttat caaagactca
    10861 tgatatgtct aaattaccaa caggtgtcga gattcgaggt aaatacattc gcatctggtt
    10921 catgtttcga ggaaaacgat gtcgggaaac attgaaaggc tgggaggtta ctaacagtaa
    10981 cattaaaaaa gccgggaatt taagagcgtt gatagttcat gaaatcaatt ccggtgagtt
    11041 tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga caacgagggt
    11101 cataaaaacg ttcggggagc tttgtgatat ctggacaaaa attaaagaaa cagagttaac
    11161 aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcaggataa taatttgtga
    11221 gagtactccg atatcgcata ttcgttatag cgatatctta aactaccgga atgaactgct
    11281 gcatggagaa acgctttacc tggataatcc aagatccaac aaaaaaggaa gaaccgtgcg
    11341 cacagttgat aactatatcg ccctgctctg ttcgttgtta cgttttgcgt atcagtcggg
    11401 atttatatca accaaaccat ttgaaggagt aaagaaatta cagagaaaca gaataaagcc
    11461 tgacccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag gacagagcca
    11521 gaacttgtgg aaatttgccg tatactccgg gcttcgtcac ggggaactgg ctgctctggc
    11581 gtgggaggat gtggatttcg agaagggagt tgtgaatgtc agaagaaacc tgacgatact
    11641 ggatatgttc ggtcccccaa aaacaaatgc ggggatccgg acggtaacac tactgcagcc
    11701 tgctcttgaa gcactgaagg agcaatacaa actgaccggg catcatcgca aaagcgaaat
    11761 caccttttat catcgggagt acggcagaac cgaaaagcaa aaactgcatt ttgttttcat
    11821 gcccagggtg tgtaacggaa aacaaaaacc ttattactcg gtaagcagtt tgggggcaag
    11881 gtggaatgca gcagtaaaac gtgctggtat tcgccgccgt aatccgtacc atacgcggca
    11941 tacttttgcc tgctggctgt tgacggcagg agcgaacccg gcatttatag ccagccaaat
    12001 ggggcatgaa actgcgcaga tggtgtatga aatttacggt atgtggattg atgacatgaa
    12061 cgacgaacag gtagccatgt tgaatgcgcg gttatcatag ttgcaaagtt tgcccccaat
    12121 ttgccccatt tagtaccaga gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa
    12181 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
    12241 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
    12301 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
    12361 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
    12421 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaata cttacggtat
    12481 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc gccttcgtcg
    12541 cggtatcctt tatcgtaccg caacggaact gggggcgacg aagatcgcgt tgggtcacca
    12601 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
    12661 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cgctggccta
    12721 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
    12781 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
    12841 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
    12901 gccgtcgcat ctgtgcgata ccaacctgtt cgattttaaa ggcattaccc acggttctga
    12961 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
    13021 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cgactgaatg tggttgaagt
    13081 gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga
    13141 taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg
    13201 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
    13261 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
    13321 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
    13381 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
    13441 tgccatttct gcttccagcg gcacaatgga actattagct ggcggcgttt gccagttaag
    13501 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
    13561 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
    13621 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
    13681 acgcgcagca acatcagtag caaccagtac acgggcgcta ccgttagcaa aacgtaccag
    13741 agtctgatcg cgatcgcgtt gctccagatc gccgtgtaac gacaatgcac tttgccctac
    13801 ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacgca
    13861 agaggatggc tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttgctgga
    13921 tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc
    13981 caaaggatcg cgttgtactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga
    14041 aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct
    14101 aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag
    14161 tgataccgtg cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg
    14221 cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa
    14281 aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc
    14341 cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg
    14401 ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg
    14461 aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa
    14521 ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa
    14581 agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca
    14641 gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg
    14701 ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg
    14761 acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct
    14821 taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc
    14881 gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca
    14941 acgcccatcg cttgacgttg cattcacctg caatggagat attgccctga atgggtagat
    15001 agtttattga cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg
    15061 cttttgttac aaccatttac tacggtgcaa ccataaagca acaccaccaa taagaacaac
    15121 taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag
    15181 gttgacgcca aataaccctg tcagaaaggt actgggtaaa aagaccattg ccatcaatga
    15241 cattgtatag gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat
    15301 cacaccagtc cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc
    15361 aatatcctgc atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag
    15421 acgagcatag acatcacgtt gcggtgccat atagcgacgc atcacgatta attgtttgcg
    15481 cagcagagcc aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc
    15541 gataatttta tcgtgcagct gctcgataaa ttcgctggaa tgatcggtca acgcatcgca
    15601 cacatccacc agccaccctc cgcaatcggt cggacccgtg ccctcttcca gatcgctcac
    15661 cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat
    15721 atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg
    15781 caatgtaata agcgtgcctt caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc
    15841 cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc
    15901 atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt
    15961 ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac
    16021 attaacgtcc gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt
    16081 agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta
    16141 ataaaattct gtctaaattg aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt
    16201 gaagtgttaa atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt
    16261 gcgatcggat agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact
    16321 tcaataccct ggacttactc accagtcctg tctggattgt ttcgcccttt gaggaacagt
    16381 taatttatgc caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac
    16441 gaaccggacc ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc
    16501 aaaaccaaca cgatattatc gaaatcctca ctgttcagcg taaagaagag gaaacagcat
    16561 taagctgtcg gcttgttttg cgagagctga cagaaacaga accggtgatt attttcgaag
    16621 gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca
    16681 aaaaacaagg tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg
    16741 acccctcacg agatggccaa atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt
    16801 ataaccatga aacgatgtgc cagaaacata cctgggaaat taatatgctc gggcgtcgcg
    16861 tcatgcctat catgcatgaa atttcgcatt tacccggcgg tcataaacct ttgaattttg
    16921 ttcataaact ggcggatgga tctactcgtc atgtgcagac ctatgccggg ccgattgaaa
    16981 tttatggcga caagctcatg ttatgtattg tgcatgatat tactgagcaa aaacggctgg
    17041 aggagcagct ggaacatgct gctcaccatg acgcgatgac cggattactg aatcggcgac
    17101 agttttatca cattaccgag ccaggccaaa tgcagcacct cgccatcgcc caggattaca
    17161 gcttgttgct catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta
    17221 aaggtgatga ggtgttatgc gccctcgccc gcaccctcga aagttgcgct cgcaaaggcg
    17281 atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg
    17341 ataccgcgct ttcgctggct gaaactatcc gtgtaagcgt ggcaaaagtg agtatttcgg
    17401 gcttaccacg ttttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg
    17461 atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc
    17521 gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca acggctgcgc ttagcgtggc
    17581 gctcccagtt ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc
    17641 tgccgccatg atgcggtagc gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc
    17701 agcgcgccac atagctgcgg acaatattgg catgcgattt atcatcccgt cctttgccat
    17761 gaataatcag cacgttacgc aaaccatcag ccatggcttg ttgaatgaaa ctgaacatca
    17821 ttttgcggca ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt
    17881 taccactgcg cagcttatcc agcaccccat gttgcaaccc ttcccgccga aactccagcg
    17941 gctgacttag tgggatgatg tcgagaaatc cggtggtgag gaaattatca agctgcagcg
    18001 tgtcgatacg ctgcggcgca cgttggttac gcgttggatg ccagtggaca tcggtagcac
    18061 gtttcagcgg ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca
    18121 tgttacatcc tcccgcaatt aagagcgcga tatgataacc agaccgggtc ggtccaacaa
    18181 cgtcttaccc aaaattccag taataagttc caaatattgc cgatatttta agcaaaatgc
    18241 ttatgcatga tt
//
LOCUS       DAZUFB010000096        17994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZUFB010000096 DAZUFB010000000
VERSION     DAZUFB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.096"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..146
                     /locus_tag="ACHY3M_003719"
     CDS             <1..146
                     /locus_tag="ACHY3M_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4318551.1"
                     /translation="PVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FNW"
     gene            158..355
                     /locus_tag="ACHY3M_003720"
     CDS             158..355
                     /locus_tag="ACHY3M_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS4318552.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            440..1282
                     /locus_tag="ACHY3M_003721"
     CDS             440..1282
                     /locus_tag="ACHY3M_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4318553.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPNNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(1563..2099)
                     /gene="yghD"
                     /locus_tag="ACHY3M_003722"
     CDS             complement(1563..2099)
                     /gene="yghD"
                     /locus_tag="ACHY3M_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFS4318554.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVLNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(2101..3279)
                     /gene="gspL"
                     /locus_tag="ACHY3M_003723"
     CDS             complement(2101..3279)
                     /gene="gspL"
                     /locus_tag="ACHY3M_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFS4318555.1"
                     /translation="MSSMLEIFFPLCASDPIRWQRHTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDISRKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(3276..4253)
                     /gene="gspK"
                     /locus_tag="ACHY3M_003724"
     CDS             complement(3276..4253)
                     /gene="gspK"
                     /locus_tag="ACHY3M_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFS4318556.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ASFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(4250..4855)
                     /gene="gspJ"
                     /locus_tag="ACHY3M_003725"
     CDS             complement(4250..4855)
                     /gene="gspJ"
                     /locus_tag="ACHY3M_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFS4318557.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(4852..5223)
                     /gene="gspI"
                     /locus_tag="ACHY3M_003726"
     CDS             complement(4852..5223)
                     /gene="gspI"
                     /locus_tag="ACHY3M_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFS4318558.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(5220..5783)
                     /gene="gspH"
                     /locus_tag="ACHY3M_003727"
     CDS             complement(5220..5783)
                     /gene="gspH"
                     /locus_tag="ACHY3M_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFS4318559.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(5787..6242)
                     /gene="gspG"
                     /locus_tag="ACHY3M_003728"
     CDS             complement(5787..6242)
                     /gene="gspG"
                     /locus_tag="ACHY3M_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFS4318560.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDIYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(6259..7482)
                     /gene="gspF"
                     /locus_tag="ACHY3M_003729"
     CDS             complement(6259..7482)
                     /gene="gspF"
                     /locus_tag="ACHY3M_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFS4318561.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(7482..8975)
                     /gene="gspE"
                     /locus_tag="ACHY3M_003730"
     CDS             complement(7482..8975)
                     /gene="gspE"
                     /locus_tag="ACHY3M_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFS4318562.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVDMTFARRLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(8975..11035)
                     /gene="gspD"
                     /locus_tag="ACHY3M_003731"
     CDS             complement(8975..11035)
                     /gene="gspD"
                     /locus_tag="ACHY3M_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFS4318563.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(11065..12024)
                     /gene="gspC"
                     /locus_tag="ACHY3M_003732"
     CDS             complement(11065..12024)
                     /gene="gspC"
                     /locus_tag="ACHY3M_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFS4318564.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(12042..12452)
                     /gene="gspS2"
                     /locus_tag="ACHY3M_003733"
     CDS             complement(12042..12452)
                     /gene="gspS2"
                     /locus_tag="ACHY3M_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFS4318565.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(12518..13327)
                     /gene="pppA"
                     /locus_tag="ACHY3M_003734"
     CDS             complement(12518..13327)
                     /gene="pppA"
                     /locus_tag="ACHY3M_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFS4318566.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGVVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(13475..>17994)
                     /gene="sslE"
                     /locus_tag="ACHY3M_003735"
     CDS             complement(13475..>17994)
                     /gene="sslE"
                     /locus_tag="ACHY3M_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFS4318567.1"
                     /translation="SATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTP
                     EPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTF
                     TPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSL
                     VTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKA
                     PSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVAGV
                     NYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANI
                     DQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNLPN
                     EFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYK
                     SVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKN
                     ELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYS
                     WNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFKRH
                     GQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPL
                     RADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMAL
                     NKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKP
                     DDKPKLEVASWLEDVDGKQETRYAFIDEVDHKTEDSLKAAKEKIFAAFPGLKECTNPA
                     YHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHEL
                     YFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYAN
                     DAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWD
                     LNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNA
                     NVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNS
                     STNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTG
                     GLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSG
                     YPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDR
                     YLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPE
                     GELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLCAS
                     WVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLKLPKPEQGPETI
                     NKVTEHKMSVE"
BASE COUNT         4030 a         5102 c         4525 g         4337 t
ORIGIN      
        1 acccggttct gcggcttaaa cgcctggggc tgtcaaaaat gttccgccgt ctggttgtca
       61 cacttatccg gcgttatggc gtcagtctca ttcatctgga tgccagtgct gaatgcctgc
      121 cgggtttacc tactttcaac tggtaaccag aaacaacatg aaatcattaa ccactgaaac
      181 agcgctggat atcctcattg cgtggctgca ggacaatatc gactgcgaat cgggaattat
      241 ctttgacaac gatgaggata aaacggattc ggcagcactg ttgccctgta tcgaacaggc
      301 ccgggaggat gtccgtactc ttcgtcatct gcagcttctg caccagaacc ggtaaatatc
      361 actcaccata cttcattcca ctgacgtcag cctgaacagt gctggcgttt tcgtttataa
      421 accaaaaggg aaaccgataa tgtcagacat cacaatctcc cgtccggaag tggtcaccgg
      481 gcatacggac gttatctgct caaccagtat ccgccacatt ctggctgtac gaaagagtac
      541 actactgcaa atcgacacac ttatccggca actggctgaa atatcagcaa tgacagaaag
      601 tattggcggt aaagccgccc cggactgggc catgaaacag gattttcgct gcggctgctg
      661 gctgatggag aaaccggaaa ccgcaatgaa agccatcaca cgcaatctcg atcgcgaaat
      721 ctggcgtgac ctgatgcaac gttcagggat gctttcctta atggatgcac aggcccgtga
      781 tacatggtac cggtcactgg agtacgataa ttttccggaa atcagtgaag cgaacattct
      841 gagcacattt gaacaactac accagaataa ggatgaggtg tttgagcgag gggtgatcaa
      901 cgtcttcagg ggactgaact ggaattacaa aaccaattgc ccctgcaaat ttggcagtaa
      961 aattatcgtc aacaacctgg tgaggtggga ccggtggggg tttcatctta tcaccgggca
     1021 acagactgac cgactcgtcg acctggaaag aatgctacat ctgttcagcg gcaaaccgat
     1081 ccccaacaac cgggaaaaca tcacgattcg tctggatggt cacatccagt ccgttcaggg
     1141 taaagagcgc tatgaagacg agatgttcat cattaaatac tttaagaagg gatctgcaca
     1201 catcactttc aaaaggctgg aactgattga cagaattaac gatataatag ccaggtactt
     1261 tccttctgtg ctctcagcct gatcccgagt gtgattccct ttcgatatca aaagggactg
     1321 cgggtataat tgagggtaca tttttaatta aaccaaacaa attgtattaa tatcaatatg
     1381 ataaaagaaa ttttcgattc cgagtccggc accactaatt cttaagaacc cgcccacaag
     1441 gcggtttttt gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc
     1501 tgggggaatt actctcgcca tgttaacgag agtaatttta ttgatactaa tctcctgata
     1561 ctttaccccc gtccaaactc cagccgctgc acattcacca tcccagtctt ctcagcagca
     1621 ctggcatcaa tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc
     1681 agcaggtcat ttaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct
     1741 atccgcttga tgactaccga gtgcgcggaa gcgctgtcac tgataacccg cgatacctgc
     1801 gcaggcgttg ttgtggcgga ttttcgcgcc gccataatat ccggcgcggc gctcctcagt
     1861 ctggtgttca tcgccaccag ttgctgcaac attgtctcct gttgttctat ccgttcgctc
     1921 aacggctgcc agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt
     1981 aacatgccct tttcacgcgg agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca
     2041 cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg
     2101 ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaatgcgtc
     2161 ctgttgcaca acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt
     2221 agcagcccgc aactggaggt gaagcgtctg gcgtttttga tcaaaggtga aaccacgcat
     2281 ttcgatgtcg gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag
     2341 cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg
     2401 taaattcaca atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt
     2461 gcggctttgc gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag
     2521 cgcaaccaga atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg
     2581 gtagctgacg cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt
     2641 ggtaatggcg ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga
     2701 tagcttttcc ggtgcaatgc cgactacggt tagcgaaagc ggtaaattct gctcattgag
     2761 ctgggcgcgg aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc
     2821 gatgcggcag ataacccgtt gtgcatcgcc agccataaac ccacaaggaa tggacatcca
     2881 gtccggcgcg acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg
     2941 catatgctgt tggtgaatca cggctacggt tgccagttgc tgattgattt tcaacggggc
     3001 gaaatgcagt tcgtcaatat cctgattcag ctcttcttcc agtaaggcag gcagaatagt
     3061 cggtatctgc ttgcgggaga tatcaggcag ttcaacctgc cagacgctga tccattcgcc
     3121 gggaatgtag agtcgaatcg catcagtttg cagccattgc tgaagctgtt cgtcagcaac
     3181 gtcaggccag ataccgtgct ccacgtcggg tgtatggcgc tgccagcgga tggggtcgga
     3241 ggcgcaaagc gggaaaaaaa tctcaagcat ggaactcact cactttctcc tgtctgatgc
     3301 cagagaaccg aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca
     3361 gtctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg
     3421 gtttttagct gttttttagt acgctcatcg acgtcagcga gtagcggctg tgcaagaaac
     3481 tgatcgacat cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaaca
     3541 gggcttaacc acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta
     3601 ttgatgttga tttgctggcg ggtcatcggc agcgcacaga ccagcggttt cagtttttga
     3661 taaagcccgg cgtccattcc ctgcaccacg cgcatctcgc tgatatcggc cagcggttga
     3721 ttggcggcgt agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccaggcgc
     3781 gtctgcacgc tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc
     3841 cgataagcag gcacatcgag gcgcgagatc agggcaatca gttgttgtac cgcgagcgga
     3901 cgcgacgccg tcgtcggctg agcgagggca ttcaggttaa agctggcctg tgcgtcacgc
     3961 agagtgacgg cgatttgccc ctgcggcagt gggaaaaaac gagggccgga agcccacggt
     4021 tgtgccagat gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg
     4081 ctttctgcac tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc
     4141 ccaagttgct gctgcatccg tccggaaagc gtgatggtta ccagcatcat aaccgccagc
     4201 aataccagca ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgataatt
     4261 gcggcccgcg taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagagtca
     4321 tgcgcactgc cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtaccat
     4381 catagaactg caaacggagc gaatccgccg gaatcaactt ttgcattgtc ggcttcacgc
     4441 tgcctgcggc atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt
     4501 aaccgacggt gagcagattg ctgcgtggca gacgcatcaa cgggttaacc acgccgccgc
     4561 gcacaaagcg catgccttca ctctcagacg ccagcacgcc agcgcccgcc agtaacgctg
     4621 gttcacgctg gccctgttcg cctcttaccg gacgcggcat catttgtgtc agatcgtggg
     4681 tcagaaaact catcgtttgc tgcatgaggt ttagtttttg atcatgtccg gcgacggcgt
     4741 tattcacgcg tgtgacaccg tttgtcacct gctgcgccat cagtgccagc gaggcaaaaa
     4801 tggcaattgc caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg
     4861 gcctcccacg gcgctaaacc aggcacgtcg tgactgaatc accgacgaaa agtcttcgtg
     4921 acggctgact tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt
     4981 tcgccagtac cagcggcttc ctgccatatc gctctcgccc tgctgcctga ccgcccgctg
     5041 ctcgcgggtc atcaggtaga gcagcgcggt ctggttttca gccacccagc ccgctaccgt
     5101 tttttcctca agaatttgct gattactcag cgcaccgctg gcaatctgta acaccgccgt
     5161 ggcagccagc gcaaaaatcg ccagcgcgag catcacttcc agcaaggtaa atccgcgctt
     5221 catggcatcc tctcatcaca ttgattgagg gataacgcgc catcgtgcgc cagttttacc
     5281 gcccaacatg cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca
     5341 aaaggcgaaa aacggatctg cggcgtcttc tttttcgcct ctttttgcag ctccagttca
     5401 atatcgtgca gcgtcaggcg acgacgttgc agctccagcg catactcctt ctgccagata
     5461 tcacttccgg gttgtaacag catctgcacc tgttttggca ccgtaacctg tgccgataac
     5521 cgggtcgcgg aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc
     5581 gcgtcgatta gcacaccgag tgtctgccct tcaatcactg ccctgtcctt aaactgcgca
     5641 aagcgagtca gaaaatcctg tgccgctttt ttcgcgggcg attcagaatc ggtcgcaaaa
     5701 gtctgtacca cgcccgcact ggcaaggccg ataagaaaaa tcaccagcat gatttccaga
     5761 agtgtgaatc cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata
     5821 tctgcgccag cgccttcccc gttttcctga ccatccgccc ccagggtata aatatcaaac
     5881 agcccttttt cacccggact gagatactga taatcattgc cccacggatc ctttggcagt
     5941 cgtttaatgt atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg
     6001 atcagcgcct caagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata
     6061 tccagtgcgt tttccagcgc cacgatatcg ctgatggctt tttgccgatc ggctttctct
     6121 ttgttgccca acaggttagg caccaccaga cttgccagga cgccaagaat aacaatcacc
     6181 accatcactt ccagcagggt aaaacctgcc cgtggttttt gtgtgcggga taacgaattc
     6241 atgtgataac tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag
     6301 gatggcgatg acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa
     6361 cagccccaac gccagcccca cctgggtatc aaattcccgt tcctgattaa cagcggcctg
     6421 ctcaagcatg gtttccagtt cgccgctctg ttcgccggag gcgatcatgt acagcatcat
     6481 cggggggaac aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg
     6541 atctgccgcc agcagtagtt gttgttcgac atagcgattt gccgacacag cggcagcggt
     6601 ctgaatacct tccagtagcg gaacactgct ggcggtgagg atgcttaagg tgcgggaaaa
     6661 acgcgccgta ttcagcccgc gcgcaacacg ccctgtcacg ggcagacgca gcacggtttt
     6721 atcccagcgc aggcgcatag taggattttt aagtagccgt tgccccagca ccagaagcgc
     6781 cagcaatccc gccagccagt aaacgccgct ggcctgtaac gcgtcgctca tggcaataag
     6841 cgtgcgggtt gaggcgggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg
     6901 caccactgcc gtcagcaaaa tagtgactac gcccgttgcc accaccagca gaaccagcgg
     6961 atagagcatg gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc
     7021 caggcgatta agcaccacgt cgagatgccc ggatttttct ccggcagcaa ccatcgaaca
     7081 aaacagagaa tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tgtaaccttc
     7141 ctgaatccgg ctgcgcagcg ccattccgag gctttttaca tgcagttttt cactttgctc
     7201 actgaccgcc tgtaagcagg tttccagcgg cattgctgcc tgtaccagcg ttgccagttg
     7261 gcgcgtgaac agcgcaagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg
     7321 caacatcccc cctgacgaag tattcatccg ggcttcaata tgcacgggga taagctcttt
     7381 accgcgcaac aactggcggg catgacgcgc ggaatccgcc tcaatcatac ctttggtttt
     7441 gcgaccatta cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc
     7501 cgcagaactt catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg
     7561 atacccgcag agtgttgtcg gacataacgt tccagctcca gctccccggc ctgacggtgg
     7621 atcaaatcac gcaatgtggc atccaccacg atcagctcat ggatggcagt ccgtccgcga
     7681 aaacctttgt gattacaggc gggacagccc tgtggatggt acagagtgac ggtacgggcg
     7741 tcggtaattc ccagcaggcg tttttcttcg tcagtggctg gtgcgggctg gcggcagtcg
     7801 ggacacagcg tgcgaaccag tcgctgcgcc atcacgcccg tcagactgga agagagcagg
     7861 aaaggctcca cgcccatatc ctgcaaacgt gtgatcgccc ccaccgctgt gttggtatgc
     7921 agcgtggaaa ggaccaggtg tccggtaagt gaagcctgga cggcgatttc tgcggtttcg
     7981 gtatcgcgaa tttcaccgac catcaccaca tccgggtcct gacgcaaaat cgcgcgcagc
     8041 ctacgggcaa aggtcatgtc gacgcgggtg ttaacctgcg tctgaccgat cccttcaatc
     8101 atgtattcga tgggatcttc aaccgtgaga atgttgcgcg aatggttgtt cagctcctgc
     8161 aatccggcgt acagcgtggt gcttttgccg gaacccgtcg gccccgtcac cagaaagatg
     8221 ccgtgcggtt tgtgtaacag ctggcgcaac tgcgcggtca gttgctggct aagccccaga
     8281 cgctccagcg tcaggcgggc ctggtttttg tccagcagtc gcagcaccac gcgctcgccc
     8341 caggcagaag gcatggtgga gacacgcacg tcaatcgccc ggccgcccag caacagcgca
     8401 atacgtccat cctgcggcac gcgcttttcg gcaatgtcca gccgcgccat caccttgata
     8461 cgcgacacca gcagcgaggc cagtttgcgc cccggacgca gcatttcatg taatgtgccg
     8521 tcaacacgaa aacggatcac cagactcttt tcaaacgtct caatgtggat atctgaagcg
     8581 ccttctttga ttgcctctgc cagcatggcg ttgatcagtt tgatgatcgg cgcatcgtcg
     8641 tcgctttcca gcagatcttc cgtttccggc agttcttccg cgagggtaaa aaagtcctcg
     8701 gcagaaccga gatcttccat cagttggcga gcctcggaag agtcccgctg ccagaccgca
     8761 ttcagccgct gttcaaattc ggcttcgtcg atttgccgca gcgtaaaggg cgcgttcagc
     8821 ccccgctgca gctcctgcaa aacagagagc gacaacggat gaacatggag gatctccagc
     8881 gacgcttcgc accatgccac caggctaaac cgacggctga aactgtaggg cagacgcacg
     8941 gtgttagcgg tggtttcctg tgctacaggc accattaacg cgttctcccg gcattaagga
     9001 acgcacgaac ttccggcggt agagcctgat tttgcgctgg cagtaccggc tgcgcggtgt
     9061 gcggcatcag gcttaaacct tgctcatcac gatagatctg ttcggcgcgc atatagttat
     9121 atttgcgctg cgacacgccg tctgccgcca taccgtcacg cagaatggtc ggacggataa
     9181 acaccatcag gttacgtttt tcttttttat ccgccgtcga tttaaacagg ttaccaatca
     9241 atgggatatc gcccagcagc ggcactttcg ccacgctttc tcccgcctga tcgtccatca
     9301 gaccaccaag cacaatcagc tcaccatcgt tagccagcac ggtggttttc agtttgcgct
     9361 caccaaacac cacgtcgagg ctggtctgtc cttccacctt cgagacttcc tgctcaatca
     9421 ccatctgtac cgcgttacct tcgttaatct gcggcgtgac tttcagcatg atgccgactt
     9481 ttttcctttc tactgtattg aaaggattgc tgttattgga gccaacggta gagccggtta
     9541 ataccggaac gtcctggccc accatgaaga aggcttcctg gttgtccagc gtagtgatgc
     9601 tcggcgtgga gagaacgttc gagctggagt cgtttttaac cgcctgcacc agtgccatcc
     9661 agtcgccttt caccacgcca accgccgtac cgctaaatcc agaaagaagc tgggcgagtg
     9721 tggagagatc gccgttggta tccggattaa tggtggtagc gccgttttca ctgatcaccg
     9781 tcgagccttt ctgcggtttc gcctgagaaa tggctgcccc cagcgtaccg atagggatct
     9841 gcgtaccatt agcaaactgc attaatccgg catctttcga cgcccactgc acgccgaagt
     9901 tgatattgct accttcggca acttccacga tcaacgcctc gacgtgcacc tgagcacggc
     9961 gaatatccag ttgttcaatc acgctttgca gcgactgcat gatgtcctgc ggtgcggtta
    10021 caatcagggc attactgtgt ttgctggcgg cgatggatac aatctcacgc ccgctaccga
    10081 ccgtgccttc cgcctcttct ttagccgccg tgagcgtgcc gctgacctgc ttcagcacat
    10141 cgaccagatc ttcggctttg ctgtatttga gatagaaaac ctggctgttg ccgctgcgtt
    10201 ccatttctga gtccagccga cggatcaggc ggcgcatttt gtcccgcgtg gccgggtcac
    10261 cactgacaat cacactgttg gtgcgttcgt cggcgacaat ttgagatttc agcgtcgctg
    10321 gctggttctc gccgctgttt ttcgtcaggc tttccagcac gcgggcgatt tccgaagccg
    10381 aggcgttatc cagcgggatc acctcttcgg tgcgattacc cgcatgatcc acacgctgga
    10441 tcacttccgt cagccgctcc acgacggagg cgcgcccggt gagcataatc acgttggagg
    10501 gatcgtaatt aacaacattg cctgagcctg cgctgtcgat catctggcgc agaatcggtg
    10561 ccagctcgcg taccgacaca ttacgtaccg gcactacttt ggtgaccatt tcatcgcccg
    10621 cgtagttgtc gctgccttca ccgaccagcg gcagcggctc gactttcgca gcgctggatt
    10681 tcaccacctt cagcacgtcg ttttccatcg gtacgacggc atacccctgt gcttccagca
    10741 ggttaaggaa tagctggtaa tactggcgtt cattgagcgg ggtcatggtg cgaatactca
    10801 ctttcccctg tacgcccggc cccataatga tggttttatt aaggttagcg ccgacggttt
    10861 cgatgaacga tttcaggtcg gtatctttaa aattagcggt gaaagtggct tcttccgccc
    10921 agaccggtga actgcataat gccgctgcca gcaccagcgg cagtaaacgc ttttttgttt
    10981 tgaggccagt tgtcttctta cgccagaccg acaacgtaat atcacgccaa aacacgatga
    11041 atgattctcc tggattaaat gcggttagcg cagcgcgatg gaaatgtcgt ggcgcgcacc
    11101 cttgcgtaaa accgtaagtt gaatggaatc cattgaaggt aactgccgca tcagagcaat
    11161 cattgctcgt ggatcagtga aatcctgctg atttagcgca atggcgatat cgccttcctt
    11221 gaaaccgctg gcatcgaaca gagaacgatc tgcccccggc ttcactgcat aaccgacaat
    11281 tccctcctta cgcacaggcg taagctggat atagttaaaa attttctgcg gatctttcgc
    11341 cagtgcctga cgcacggcag tcggtatctc aactggcgca ctgacagcag gttcagcaac
    11401 agcttgcttt gcttcgtcac tgacagcttt tttgttggtc gcggcaacgg tggaacgctc
    11461 ctcttcagcc aggctcaggc gctctatttt tccctgatag cgcagcatca catggtcgcg
    11521 attgatttcc tcaatcaccg cattgtgcga gccaagcgtt tcaccctgca aatagacctg
    11581 ctgtttaccg ccttcttcaa taaccgcgcc gggtctggca ccaaaggcaa tcccgcgcag
    11641 caccacatta agacgcgttt ctgccacagg cacaggttcg ggttgtttta ccggcgtggc
    11701 gacgggctga tatttgccaa accagttttg ctggctgatt aattgcacgt catttttatc
    11761 gaacgttttt gcatccgcac ggggcggttt attcgccgat ggggaaacag ccgtatattc
    11821 cgcagaaaag gagaaatagc gccagagtga atgcgccact tttgcagaaa taataagcag
    11881 catcagccag aacatcccgc gtacaatttt tcgcagatgc tctttgtctg tattaaaaga
    11941 ttgtctctgg ttaagagtgt gacgaatttt tgtcagccat tgaatgagat aaattcgtgc
    12001 gtcacgaaaa acaacccgcg ccaatttata tctacccgac gttatgcttt gactattcca
    12061 caggtggtac gatccagttt ccgctgatac tggttacctg tacgtgtatc attaatcgtt
    12121 atgctgtaat taattccctc ggtaatcatt aatttcaccg ttgggtcagc gcacatttgt
    12181 cgttgatagc tggttaaaaa ggcgtcaggc gtctgcgtgg tctgagtccc cgcttcgctg
    12241 ataatggtca tttttaccgt cgtgccgcta ctttgtgcca gcactaaggt atatcccgca
    12301 gatttaatcg gcaggttttg gctgatattt tgcgcctgtt ttttcgccag taaactggca
    12361 ttttcattat ggctggcaca gccacttaat aatcctgtaa cgctcaacaa tagcgatatt
    12421 aataccctcc ctggcatttg ttttatcgac atggtaaata atctctaagg ttattaataa
    12481 gagttaaaat gtcactttga taatgacgtt gttatcatta aaacaatgcc tgtagataaa
    12541 ttgttgctat accgcccaga cttaaacacg gtccaaaagg cagtgtggtt gatcctcttt
    12601 ttgtaataac ggcatatatc aggccgcagc atgatgcgat taaagcaaca ttgggtagcg
    12661 acaacggccc cacccaacta cctaacgcag cgaacaataa tacatcgccc atgcctaatg
    12721 cttctttacg cagaactact ccggctatcc agcgcaggga gtaaaaagcg ataaacccca
    12781 ccaggacgcc ggtaactgca tcttgtagcg tgagcgggct ctgctgcgcc catgccgcaa
    12841 tcagtcccgt ccacaatacg ccctgagtaa aaacatcggg cagccattgg tgatcgaggt
    12901 caatgacgct cgcggcaatc agccaggcgg ataatatcat caccgccagc gcccatccac
    12961 tttccggcca gaccagactc gccagcaaaa aagcgagtgc cgtcaataac tccaccagcg
    13021 gataacgctt gctgattttc gcctgacaat cacggcagcg ccctttgagc atcagccagg
    13081 agagcagcgg aatattgtca cgtatccgga tggtctgctg acaatgtggg cagtgggaac
    13141 gcggtagcgc aaggcttatt tttgactgcg cactcggcat ttcaccatga aactccgcca
    13201 tttgttggcg cagcatgatg gggtaacgcc aaatcaccac attcaaaaaa ctgccgatga
    13261 tcaatcctcc gacggttgcc agtatgggca tcgccgcggg gtattgctga aaaacatcaa
    13321 aaagcatggt taaaggttgt ttgttgtaac ttgccggatg cgggcctgca agtgtatgcc
    13381 atacggcttt cctccaggcc cgataagctg gcgcaacccg gtgcgcctta tttcatgccg
    13441 gatgcggcgt gaacgcctta tccggcatac aggattactc gacagacatc ttatgctcgg
    13501 taaccttgtt aatggtttcc ggtccctgtt ccggtttcgg cagcttgagt gacgccagcg
    13561 tgttgtacgc cgactggctc acaccgccct cgaagctcat ctcgctcgcc cccggcagct
    13621 ggtaagcatt cgcacccgga ttccatttct taaagaactc cgaaagatcc gtctgggcga
    13681 cccaggaggc acacagcatc agcgtgtccg cagcgttacc gttggattca gcacagtaat
    13741 tcttgccacc aaaggtcttg tcaccaacgt catcgccgcg cgctttacgg tgcatcaact
    13801 ggaacaggtt ccagcctttc atcccttcac gatcgctgaa gaacttcggc aattcacctt
    13861 ctggatacca tttcttgata tcaaagtttt tctctgccca ttccttcagc tgcgcgtaca
    13921 tcagcagacg gtcacccgca ccgccgcgcg cccatgcctg accgttgctc tcctccagat
    13981 attccggtgc gacggtaata tcgtcagcga cacggttcat cttgccgaga taacgatcct
    14041 gcatgtacag cgccagcacg ttgttcgcca cttcggtcgc gcctggaaca gtcagcggcg
    14101 tttctgcggc gttgtgccct acttcgtgcc agatcagcca gtcgttcagc ggcgtcgtcg
    14161 gcagcgtggt gctgttcgtc gagaagctgc tgttcattac cggataaccc gagtgcgcat
    14221 caccgatgga gatctgcaca tcgtttgtga aacgatgttt atgtcccgtc aatgctttat
    14281 aggtaaacat ccggtgctta ccgctcgttt catcacgacc gtagaagtca ttcatcgagc
    14341 tggcaaaggt gtccagatcg ttagcgaatt gctccagtcc gccagtgtaa ttgctggcat
    14401 tcaggttctt cttcggtgtg gtatagacga aagcgtctga ttccagctca cccagcggag
    14461 ccggtgagtt cagatcgttt ttccatgcgc cgtctttata gaacggtgct tttaccacgc
    14521 cagtaaaggt gaagctggca gattcattgg tagagctatt gcccttgata taaatcaggc
    14581 caccgtaagg caccttgaac ttcaccgtac cgctagcgtc cagagagtac gttttagtca
    14641 ctcttggcgg acggttcagc gcaacttcat gcttctcacg tccggtcagg tcgtcagcca
    14701 gcgccacggt gacggtcaca ggaacgttcg cattggactt aatggtgacc tctttctgag
    14761 ccggtgccca caggccagtt gactgcatgt tacctgcaaa ccatttggtc ggattcgagt
    14821 acaggctgat ggtttcagta acgttctgtc cctcttccga tactgctccc ggatacttct
    14881 cgacatcaac tttgatgttc agatcccacc aggaacggcc cagcatcagg cgtgtcagcg
    14941 gtttttccat atagttgagc gggtagctcg ggttcatcat gcccgctttg ctgctaccgt
    15001 caccgtagat catgttgtta tcgaccagcg attttttcag atctgcagaa cacttggtgc
    15061 cgcctgcata ggcatcattg gcgtagcagt tcaggaactc ggtgaacgtt ttaaagccca
    15121 gctcgtcatt tttgccttct tcataacgat agctcgtatc gttccacagc cagaccgaca
    15181 tgttctggta cagacgttcc agatcgacgc tgctcagacg ctcacctttg cgaccattgg
    15241 tccggaagta gagctcatgc tgatacagac gctgaatgtt ggtgcctaaa tccgcagcct
    15301 gcaccatcgc ttttgcagtg tcggcgttaa ggcttagttg cgtatactgt ggaacataca
    15361 tgccaccagt aaccggaacc cccgtgccag gacgatattc caggcagttg acctcatagt
    15421 gatatgccgg attagtacac tctttcagcc ccgggaacgc ggcgaaaatt ttctccttcg
    15481 cagccttcag agaatcctct gttttatgat cgacctcatc aataaaggca taacgcgttt
    15541 cctgtttgcc atctacatct tccagccagc tggcaacttc cagcttcggt ttgtcatcag
    15601 gtttgttttc tacctgatat ttccacttaa cttcccctgt cttactatcg atggtgtacg
    15661 gcagcgcacc atctacggca ggataacgtt catagaccca aatgcccgtt gcgcgctgct
    15721 gacgaacgcg gttcggatac ccttgcggat cgttatttac taccgacttg ttcagtgcca
    15781 tcgacagacc tgcggcatcc aacagacgca caaaaccaga cgcgctctct tccttaagat
    15841 tgctcatcac gttttccatg atcagcaccg atccaccttt gttcagatag gcgatcagat
    15901 cggtcacatc ctgctgagtc agcttcggtt tgctggtatc tgcacgcagc gggattgcat
    15961 aaggatcgtt acccacctga gtcacatatt caaagccgtt aaggatcagc agcggcattt
    16021 cctgcggatc gagatcgcca tagctactta aatgctcaac agagatgccc gcaaaatccg
    16081 gatggaagtc gaacgcagcg ctgtttcctg taacctgacc atgacgttta aaatagacag
    16141 tatccaggtt ggtgcctacg gtcatgctgg ctttcgcgtc cggcgtccat ttatcatcgg
    16201 acagatagcg cagcacgttc tccatgaagt tcttcatgtc atccgggtcg ctgttgagcg
    16261 tacactgccc atctttatta acgcccccgt tccagctgta accgttcggg caacgcaaaa
    16321 tgctgttgta gtgtgggttt ccgataacca tcagtttgcc ctcaccgact tgccccagcg
    16381 aaataaacgg caggttgaaa gtggcggtat cgcgcgtaac gttttccggc tcaacaagag
    16441 aaggcgcttc cgtaatgtac gccagctcgt ttttatccca ggcgcgtttt tcgccaaacg
    16501 ccagccagta gtttttatca ttacgcgcca tcagaatcgg gaaggccgcg ttggagatat
    16561 tcaccaccgc ctgaccgcgc gcattacccg tgctgccata gaagttagta gagtcatgga
    16621 atacatggaa cttgctgaca gatttgtagt tcgtatccac gccccacagc ttgttgataa
    16681 cgccctgaat ctggccgtca ttaacattgc gcgtcgtcag cgagaaccag cgagcctcgt
    16741 tacaaccgtc ggttttcgca caaatcgcgg tatcgatctc tttggcctga cccgtcttaa
    16801 actgctcgat aaattcgttt ggcagattaa caacttgctc accttcatcc agcgtcgcac
    16861 cgttggataa cgagagattg ataatctcgt taatcacgtt gggatattcg gcaaagacct
    16921 tgcgtacatc atccggaaca acacgggtat tattttgccc ggtcgtcgaa tagcgatgaa
    16981 taagctgatc gatattcgcc ccgcgaactt catcacccag ttcagtcagc gcaatggtcg
    17041 acttattgcc gcgcactgaa cccagttcaa aggtatcgat accaaaggag atggtttcgc
    17101 cccagctaaa agaaaattca ccattttccc ctgtcacgcc acggcctgaa ttggtgtagt
    17161 agttgacgcc agcaacacca tatccctggc tatccaccag gcgaccttct gagagaatga
    17221 tttccgaagg ttgatactga taaaactgtt ccgcgttagc cgacacgaag gaggcgttca
    17281 gatccggttt tgttcccgga gtcgtgacgg ggactaccgg tgaagtatga gtggatggcg
    17341 ctttatcagt cgcggcattg ttttccacct cttcattgac cagctttttg aactcttccg
    17401 gtgccagatc gatttgctta tacagcgagt cgaaacgttt actctcgatc accgaggaga
    17461 actccaggca cacctgttct gtattcgccg ggcaactgtt catggaggtg accagcgaga
    17521 gcgcattgct tttcttgttg tcggaacccg ccagttcttg cgcgtcctca agactaaacg
    17581 acactttttc aaccgcacgc aggctacgcg cagcttctga ctgggtgtcg aaggtagcaa
    17641 ttgtcgtgtt gttccctgcc acacaggtga ctttgtcgcc tggtgtaaag gtaaagccat
    17701 cgctggattc atcattacag gtgatatcac cagttacccg caggcttccg cccagggtca
    17761 gataacccgt tttcgtagga acaggttctg gctccggttc tggtgttggc tcaggatcag
    17821 gtgtcggttc cggcgtaggt tctgggtccg gcgttggctc aggcgtcggc tccgggtttg
    17881 gtgttggatc aggtttcact tccggcaaag accctgttcc agaatctaca ggcggcgtat
    17941 cggaggaaga tccggagcca ccgccatcac aaccggctaa cagggttgca ctca
//
LOCUS       DAZUFB010000097        17909 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZUFB010000097 DAZUFB010000000
VERSION     DAZUFB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.097"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..276)
                     /gene="cspA"
                     /locus_tag="ACHY3M_003736"
     CDS             complement(64..276)
                     /gene="cspA"
                     /locus_tag="ACHY3M_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFS4318568.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(557..847)
                     /locus_tag="ACHY3M_003737"
     CDS             complement(557..847)
                     /locus_tag="ACHY3M_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFS4318569.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1281..1991
                     /locus_tag="ACHY3M_003738"
     CDS             1281..1991
                     /locus_tag="ACHY3M_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFS4318570.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2041..3015)
                     /gene="ghrB"
                     /locus_tag="ACHY3M_003739"
     CDS             complement(2041..3015)
                     /gene="ghrB"
                     /locus_tag="ACHY3M_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFS4318571.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3119..3778)
                     /locus_tag="ACHY3M_003740"
     CDS             complement(3119..3778)
                     /locus_tag="ACHY3M_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFS4318572.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3931..6264
                     /gene="bisC"
                     /locus_tag="ACHY3M_003741"
     CDS             3931..6264
                     /gene="bisC"
                     /locus_tag="ACHY3M_003741"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFS4318573.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6233..6673)
                     /locus_tag="ACHY3M_003742"
     CDS             complement(6233..6673)
                     /locus_tag="ACHY3M_003742"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS4318574.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQLAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6670..7233)
                     /gene="tag"
                     /locus_tag="ACHY3M_003743"
     CDS             complement(6670..7233)
                     /gene="tag"
                     /locus_tag="ACHY3M_003743"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4318575.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7391..8089
                     /locus_tag="ACHY3M_003744"
     CDS             7391..8089
                     /locus_tag="ACHY3M_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS4318576.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8318..9520
                     /locus_tag="ACHY3M_003745"
     CDS             8318..9520
                     /locus_tag="ACHY3M_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318577.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
     gene            9844..10368
                     /locus_tag="ACHY3M_003746"
     CDS             9844..10368
                     /locus_tag="ACHY3M_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFS4318578.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            10452..11138
                     /gene="lpfB"
                     /locus_tag="ACHY3M_003747"
     CDS             10452..11138
                     /gene="lpfB"
                     /locus_tag="ACHY3M_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFS4318579.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            11162..13693
                     /gene="lpfC"
                     /locus_tag="ACHY3M_003748"
     CDS             11162..13693
                     /gene="lpfC"
                     /locus_tag="ACHY3M_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFS4318580.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            13704..14759
                     /gene="lpfD"
                     /locus_tag="ACHY3M_003749"
     CDS             13704..14759
                     /gene="lpfD"
                     /locus_tag="ACHY3M_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFS4318581.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            14765..15289
                     /gene="lpfE"
                     /locus_tag="ACHY3M_003750"
     CDS             14765..15289
                     /gene="lpfE"
                     /locus_tag="ACHY3M_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFS4318582.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            15542..17233
                     /gene="eptB"
                     /locus_tag="ACHY3M_003751"
     CDS             15542..17233
                     /gene="eptB"
                     /locus_tag="ACHY3M_003751"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFS4318583.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            17325..17401
                     /locus_tag="ACHY3M_003752"
     tRNA            17325..17401
                     /locus_tag="ACHY3M_003752"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17359..17361,aa:Pro,seq:cgg)
     gene            17436..17569
                     /locus_tag="ACHY3M_003753"
     ncRNA           17436..17569
                     /ncRNA_class="other"
                     /locus_tag="ACHY3M_003753"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         4442 a         4256 c         4564 g         4647 t
ORIGIN      
        1 aaacaaataa aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag
       61 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      121 ggacactttc tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg
      181 tacgaacacg tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      241 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      301 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      361 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      421 tgtacgtcaa accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt
      481 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      541 taaagtggat aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca
      601 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
      661 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaac cctgtccctt
      721 ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
      781 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
      841 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
      901 agaatagtcg ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt
      961 cgattcagcc agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1021 tatctcttac cactcctggt ttgtaaagat ttcgaaaatc taaccttctg cgaaggcgta
     1081 aatctctggc tacaaaaaaa gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1141 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1201 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1261 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1321 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1381 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1441 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1501 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1561 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     1621 tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     1681 ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca
     1741 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggct gcacaaactt
     1801 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     1861 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     1921 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     1981 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2041 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2101 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2161 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2221 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg cagcaatcag
     2281 tgcattttca tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2341 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2401 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2461 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2521 gtgcgcacgt tgtgccagcg ccataccgat ccgtcccatc ccgacaatgc ccagtgtttt
     2581 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     2641 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     2701 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     2761 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     2821 cggcattttt tccagcaatg cggcatcaac attctcgttt gaacccagta aaccttcagc
     2881 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg
     2941 aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3001 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3061 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3121 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3181 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3241 cctgggtgat caacgcgctg gcaacggaat ccgcacgttg ctgggagaga cgcatgttca
     3301 ggtcgtgacc acccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3361 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc cgcaggtttc agggtcgcgc
     3421 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3481 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3541 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     3601 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     3661 ctgcgccgat agcagattta cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     3721 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     3781 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     3841 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     3901 aatacatcta acctttcagg agttttcggt ttggccaact catcctcacg atattccgtt
     3961 ctgactgccg cccattgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4021 tcgcatggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4081 gttcacagca atacacgggt acgatttcca atggtgcgca aaggctttct tgcgtcaccg
     4141 gaaaatccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4201 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4261 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4321 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggc
     4381 gcggcacagg cgatcatgcc gtatgtcgtg ggtggtagcg aagtttatca acagcaaacc
     4441 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4501 aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttccgca
     4561 ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga aaccgtcgat
     4621 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     4681 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctgacgcgt
     4741 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     4801 aaaaacgccg aatgggcggc agagatttgt ggggttaacg cagcgaaaat ccgcgagctg
     4861 gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa
     4921 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     4981 ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg taaccccacg
     5041 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5101 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5161 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5221 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5281 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5341 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5401 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5461 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5521 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     5581 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     5641 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     5701 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     5761 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     5821 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     5881 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     5941 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6001 tcccgtgggc agatccttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6061 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6121 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6181 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6241 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6301 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccagttga tttttttgat
     6361 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6421 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6481 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6541 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     6601 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     6661 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     6721 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg
     6781 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     6841 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa
     6901 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     6961 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7021 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7081 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7141 cagtttttta ctgtcagttt caggcacgcc ccattcatta tcatggtagg caatataaag
     7201 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7261 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7321 actatgcccg atatccttca tgcaatgaca ataatattgc cgaattcggc tcttccctgg
     7381 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7441 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7501 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7561 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     7621 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     7681 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     7741 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     7801 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     7861 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     7921 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     7981 tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac
     8041 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8101 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8161 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8221 tgatggcatt tcatgccgtt tttccccagg cataaaatgc acttcgttat ggttgtcggc
     8281 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8341 tacccgctgg ctgacactca tcggtactat cattacccag tttgcactgg ggtcggttta
     8401 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8461 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8521 attgcaggaa cgttttggcg ttaaacgcgt caccatggct tccggcattt tgctgggatt
     8581 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     8641 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     8701 gttcccggag cgtaaaggtc tgatatccgc gttcgctatc ggttcttatg gtctgggtag
     8761 cctgggtttc aaatttatcg acacgcagct gctggaaacg gtgggtctgg aaaaaacctt
     8821 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     8881 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     8941 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ccgcctgcat
     9001 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9061 tgtggtttcc gcagccaatg cagtcactgt tatttccatc gccaaccttt caggtcgtct
     9121 ggtgctgggt attctgtctg acaaaatcgc ccgtatccgt gttatcacca ttggtcaggt
     9181 gatatcgctg gtgggtatgg cggccctgct gtttgcacca ttgaatgcag tgacgttctt
     9241 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccgtcact
     9301 ggtcagtgag ttctttggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9361 tttcggtatc ggtagcattt gtggttcgat tatcgcctca ctgtttggcg gcttctatgt
     9421 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacaattcg
     9481 tcagccagag cagaaagtat tgcgtgaggc gcatatttag aaatatgttg ctactgaaat
     9541 aaatgcgagg agggagactt caaagtctcc ctttttttat ggacgcatat taaaagaaca
     9601 ataatattta caccacaaat atattttatt tgtttatata tcatgtaaat ataaatatat
     9661 tcctaaaaaa tagtcgttgg aagcgttttt gtgtgcaatt aattgaggtt gaagtacatt
     9721 tcaatttatt gcaaaaaata atcttcagta aaaataattt tcttttttag caccatttgt
     9781 ataatctgcg ccccgttgta tgaacaacat caagcatatt tatctatata aatggagtta
     9841 taaatgaaaa aggttctgtt tgccttatct gcgcttgcgc tgacctctac ttccgtattc
     9901 gcagctgatg cgggcgacgg tactgttaaa ttcaccggtg agattgttga cgccgcttgt
     9961 gttgtgtctc cagacactca aaaacaagaa gttgttctgg gtcaggtgaa caagtctgta
    10021 tttactgcta tgggtgataa atccacagca acgccgttca aaatcaaact ggaaaactgc
    10081 gatatctcca ctttcaaaaa tgtggaaatc agctttaacg gcgttggcga tgcagacaac
    10141 agcaaactgg tttctgtaag cactgaacca ggtgccgcaa ctggcgtagg tatcggtatt
    10201 tatgataaca ccaataccct ggttgacctg aatactggta aatctgctac tgtcctgaaa
    10261 gaaggtcaga ccgttctgta tttcaccgct aactatgtct ctaccaaaga tgcagtaacc
    10321 acgggttacg gtaacgctga agtcgacttc aacctgactt acaactaatc gatcctgacg
    10381 gaacgatcat ctctcaatgg caacagagac tgtgttgcca tcattcacac caatgactca
    10441 gggaccgaat catgaaacgt attagctttg ctgtgctggc acttttaatg accgcacaac
    10501 agtgttttgc tggtggcgta gccttaagca cgacgcgtgt tatttatgat ggcggcaaga
    10561 aagaagcgtc gctgaccgtt gaaaaccata ataaaaatga agagtttttg atccagtcat
    10621 ggattgatga tgtgaatggc aataaaaaaa caccgtttat tattacaccg ccattattca
    10681 aacttgatcc tgataaaaat aacgtcttac gtatcgtcaa tattgaagat accttaccta
    10741 aagatcgtga atccgtttac tggattaatg taaaggccat tcctgcgaaa agtgctgaaa
    10801 atgaaaataa aaacgtctta cagatagccg ttcgcacccg tttgaagttg ttctatcgtc
    10861 caaaaggttt gccgggaagc aacctcgagg ccatgaaaca gttacgtttc cagcgtcaaa
    10921 ataaccaaat tgtggttaat aacgactccg cttataacct gacctttaat cagttttcca
    10981 ttaatggaca aaagattgaa cgggcgggaa tggttctcgc gaaaggaaag ttaaacatta
    11041 accttccagc cggtacaggc aacgcgcacg aagtgaaata cagcattatt aatgatttcg
    11101 gtgttgctgg cgaaatgctg acgaagccga ttaactgatc cttttacaag gcgcttttac
    11161 tatgcattgg atgcatttac ctctctatca ctacagagcg catttttcct tctctctgct
    11221 ggcattaacg attgccagct cgttacctgc gtatggcgga aaatttaacc ctaaatttct
    11281 cgaaaacgtt cagggtatcg atcaacacat cgacttgtcg gtatacgact cacctgtcgg
    11341 ccagcaaatc cccggtaaat accgggtttc ggtgtttgta aacgaagaaa aaatggcttc
    11401 ccggacgctg gattttagta ccgccagcga agcgaaacgc aaagccagcg gtgagtcgct
    11461 tatgccttgc cttagccggg tgcaactgga agaaatgggc gtgcgcgttg acagctttcc
    11521 ggcgctgaag atgtcaccac cagaagcctg cgtagcgttt gatgaaatca ttccccaggc
    11581 caccagccgc tttgatttta atactcagac gctgcatctt tcctttccac aggcggcaat
    11641 gatgatgacc gctcgcggca cggttgatcc ttctcgctgg gacgaaggaa tacctgcgct
    11701 gttgctggat tacagcttta gtggcagcaa tggacgcaat gaaggtacag gttcatcttc
    11761 agattcgacc agtgacagtt attacctgaa cctgcgcagc ggtttgaacg ttggtccgtg
    11821 gcgtttacgt aataacagta tctggaaccg taccgatggc aaaaaccaat gggataacgt
    11881 tggtacatcg ttaaaccggg caattattcc acttaaatca cagataactc tgggtgatac
    11941 cgccacgcct ggcgagattt ttgatagcgt acaaatgcgt ggggcgttac tggcatccga
    12001 cgatgaaatg ttgccggaca gccagcgtgg ctttgctccg gtagtgcgcg gcatcgccaa
    12061 aagcaacgca gaagtgagta tcgaacagaa cggctatgtc atttatcgca cttttgttca
    12121 gccgggtgca tttgagatta acgatctcta cgcaacctcc ggcagcggcg acctgacggt
    12181 gatcattaaa gaagccgacg gaagtgaaca acgttttatc cagccgtttt cggcggtggc
    12241 gattttccag cgtgaagggt atctgaaata cagtctggct gccggtgaat atcgtgctgg
    12301 taattacgac agcgataaac cacatttcgg gcagtttacc gccatgtacg gcttgccgtg
    12361 gggtatgacc gcctacggtg gtgcattgtt gtcggcagat tacaacgctt tagctctggg
    12421 cctggggaaa aacttcggta ccattggcgc agtatcggtg gatgtcactc aggcgaaaag
    12481 tcagttacgc aacaatgaaa aagaggaagg ccagtcttat cgcttcctct attcgaaaag
    12541 ttttgagggt ggcactgacc tgcgtctgtt gggctacaaa tactcaacca gcggctacta
    12601 caccttccag gaagcaactg atgttcgcag tgacgccgac agtgattacc gccgttatca
    12661 caagcgcagc cagatacagg gcaatattac ccagcaactg ggagactacg gctcggttta
    12721 tttcaatatg acgcagcagg attactggaa cgttgacggt aaggaaaact ccttaagcgc
    12781 cggttatcac ggtcacattg gtcgggtgaa ttacagcgtg gcctatacct ggacgagaag
    12841 tccggaatgg gaagaagacg atcgcttgtg gtcattcagt gtttccatcc ctctgggggg
    12901 cgcgtggagc agttatcgca tgactaccga ccagaatggc aagacgtcgc aacaggcctc
    12961 tgttagcggc acgttactgg aagatcgtaa cctgagctat aacgttcagc agggttacac
    13021 cagtaatggc gtaggctaca gtggcagcgt caacatgggg tatatgggtg gctctggtaa
    13081 cattgacgta ggctataact attctaaaga taaccaacag gttaactatg gcgtgcgcgg
    13141 cggtgtcatc gttcacagtg aaggtataac gctttcgcag cctttaggcg aaagtctggc
    13201 gattgtttct gcaccgggcg cgcgtggtgg acatgtggtt aacagcagcg gcgtagaagt
    13261 tgactggatg ggcaacgccg tggtgcctta tctcacacca tatcgtgaaa ccattgtgga
    13321 attgcgtagc gacacgttgg ggcaaaacgt tgaactacag gaagcgttcc agaaagtcgt
    13381 gcctactcgt ggtgcagttg tgcgttcccg ctttgatacc cgcgtcggtt accgtgtgct
    13441 gatgagctta aaacaggcga atggcaacgc agtaccgttt ggtgcaaccg cagcgttaat
    13501 tgatgagagc aaacctgcca gcagcattgt gggtgaagaa ggacagcttt atatcagcgg
    13561 gatgccagaa gagggcgaat tgcaggtgag ctgggggaac gagcaggcgc aacgttgccg
    13621 tgtgccattc cgcttgccag agaacaagga caatgcggct atcgtgatgg ttaatgcggt
    13681 atgcgagaaa taaggatatt tagatgaaaa ttaaaaattt gttggcaggt atggtgctgt
    13741 tagggacgag cacttttgct ggaaatatat gggctgcgga ttggggacca tgtcagacat
    13801 caaatggggt ggcccatgag tatagttttg atttcgtaca gactatccag gtaccttcag
    13861 aaaataaggc tgggaaaata ttaactcagc cctttgcttt aggtacaaaa tattccgctt
    13921 attgtgaatg cccggatccc attccagata atggagttgt gacttatttt aaaggtgtaa
    13981 cgcttctgcc tgagcctgga actgtagatg ggtactataa attcaataat tacattgata
    14041 ttctcaccaa aatatatgtc tacacacaat tagatatccc ggtgcctttt accgatcgaa
    14101 gcaatggcac ggcacagaaa gaatgtacgc cctatactgc taacaattgg gggacaggtg
    14161 gtaaaggaag tatcagtatt tatatttcac atccttttgt tgggcaaatg attattccta
    14221 aaacacgagt tgcgtccctc tttgggacaa aaaagaaagg tgtttacaat gatagtcagc
    14281 ccatggccaa tgtcagtatt tccgggagta ttacagtaac tcaaggctgt gaacttgccg
    14341 ctggcaccgt actggatatt ccattcggag aatatcaggc ccatgatttt aaagggcgtg
    14401 ccggacaacc accacaaaat gttcagaagg tacaaaaaga attaagcttt gactgcaata
    14461 atatttcaga tggcgtcaaa atttatctca gtattgatgc aacaccaaat actgcctatc
    14521 ccagcgcaat tgatttaggc aacgccgatg ttggtgcagt cattgaggac ggtaaaggta
    14581 atattcttaa gccgaatgac agtaatagct tattagaaat gaacccaggc tcgctgtatg
    14641 aagatgtcaa acgcaaagcg acaaccacaa ttaccgctta tcccgttagc actaccggaa
    14701 aattacccgc tgccggggat tatagcggta ttgctaccat acatgtcgaa ctggagtaac
    14761 agtcatgaag cgaacaatac ttgcgttatg cctgctgcct ctttcctttt cgtcattcgc
    14821 ggcaaccacg gatttagggc cgaaaggaac attgaaattc acccttaaaa tcagccaggg
    14881 tgcttgcgaa ctggtgaaag acagcgttga ggttgacatg ggcaccgcgg ttctcaaaaa
    14941 gccggtaaga atcggaacgg aaattaatcc cactcctttt tccatcggcc tgaaaaactg
    15001 tagcgaagtg gtcagagcct acgtgactat ggacggtaca cccaatgcag acgactcaaa
    15061 tctttttgcg ctggatgcgg gtggcgctac aggcgttggg ctgaaaatca aaaccgctgc
    15121 tggagtacaa cagttcccgg aaaatacaac gccgacaccg gttgagtttg caattaacgg
    15181 tgatggcgca catcaactga actatatcgc cagctacgtg cctattcgcg ccgatgcaac
    15241 tattggtcgc gcagatgcaa cggtcgactt tactgtacag tacgaataat ttcacatctt
    15301 aagggccagt taactggccc tttttactgc tttttgtaac gctaaagtct cttttcaaac
    15361 ttgcattttt gtaaatttgt gcttcatgca cactctttcc ccacactttt tcgctttgct
    15421 gtggtctact tattcgcgcg tgtagatttt acttatctga ctacctccgc acttttcccc
    15481 tgccgggcct gaaaagccgc taagcagggt gttatcacct gtttgtccag ggtttgtttg
    15541 catgagatac atcaaatcga ttacacagca gaagctgagc tttttgcttg caatctatat
    15601 tggccttttt atgaatggcg cggtttttta tcgccgcttc ggcagctatg cgcacgattt
    15661 taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc gccaccgtac tggtgacctt
    15721 ctttttacta cgtcttctat cgctgtttgg ccgccgcagc tggcgtattc tggcgtcgct
    15781 ggtggtgctc ttttccgcag gtgccagcta ttacatgacc ttccttaatg tggtcattgg
    15841 ttatggcatc atcgcttccg tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg
    15901 tctgaacttt attctctggt taatcgccgt tagtgcattg cctcttatcc ttatctggaa
    15961 taaccgctgt cgctacacct tgctccgaca actgcgaacc ccagggcagc gtattcgcag
    16021 cctggcggtc gtcgtactgg cgggtattat ggtttgggca ccgattcgtt tgctggatat
    16081 ccagcagaag aaagtggaga gggcgaccgg cgttgatttg ccgagttatg gcggtgtcgt
    16141 agcgaactct tatctgccat caaactggct ttctgcgttg gggctgtatg cctgggcgcg
    16201 ggtcgatgaa tcttccgata ataattcatt gcttaatccg gcgaagaaat tcacttatca
    16261 ggcaccgcaa aacgttgatg acacttatgt cgtgtttatc atcggtgaaa ccacgcgttg
    16321 ggaccatatg ggcattttcg gctatgagcg taataccacg ccgaaactgg cccaggagaa
    16381 aaatctggcg gcgttccgtg gttactcctg tgataccgca accaaactct cactgcgttg
    16441 catgtttgta cgtcaggggg gcgcggaaga taatccgcag cgcacattaa aagaacagaa
    16501 cattttcgcg gttctgaagc agttaggatt cagttctgac ctttacgcta tgcagagcga
    16561 aatgtggttc tacagcaaca cgatggcgga caacattgct tatcgtgagc agattggtgc
    16621 ggagccacgt aatcgtggca agccggtaga tgatatgttg ctggtagacg aaatgcagca
    16681 atcgctaggg cgcaacccgg atggtaagca tctgatcatt ctgcatacca aaggttcgca
    16741 ttttaactac acccagcgtt atccgcgtag cttcgcgcag tggaagccgg aatgtattgg
    16801 tgttgatagc ggctgtacca aagcgcagat gatcaactcc tatgacaact cggtgaccta
    16861 tgtggatcac tttatctcca gcgtgattga tcaggttcgc gataagaaag cgattgtgtt
    16921 ctacgcagct gaccacggtg agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg
    16981 tgaactggca ccgccggagc agttccgcgt accgatgatg gtctggatgt cagataaata
    17041 tctggaaaat ccggccaatg ctcaggcatt tgcgcagctg aaaaaagaag ccgacatgaa
    17101 agtgccacgc cgtcatgtag agctgtacga caccatcatg ggttgtcttg gctatacttc
    17161 accggatggt ggaattaacg aaaacaacaa ctggtgtcac atcccgcaga caaaagtggc
    17221 agcggctaac taagcggcct gctgactttc tcgccgatca aaaggcattt tgctattaag
    17281 ggattgacga gggcgtatct gcgcagtaag atgcgccccg cattcggtga ttggcgcagc
    17341 ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc
    17401 aaattcgaaa agcctgctca gcgagcaggc ttttttgcat ttgaagccca tgaggatgag
    17461 aacctccggg ggcggggagg ttcgactcga gcgaagcgag agaacgttgc gccagcaacg
    17521 gcccgcaggg cgagccacga agtggcgagt aatcctctat caccgaccaa attcgaaaag
    17581 cctgctcaac gagcaggctt ttttgcattt gtagctcatg aggatgagaa cctccggggg
    17641 cggggaggtt cgactcgacg tagatactga taagacgcgc cagcgtcgca tcaggcaacg
    17701 gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac accacacatt
    17761 ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca
    17821 ttgtcttttt ttatatttgc ttaaatcttt ttttgcgtta cttatggctg gcgctggcat
    17881 tttccgctgt gcgttttagc gttcatggt
//
LOCUS       DAZUFB010000098        17774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZUFB010000098 DAZUFB010000000
VERSION     DAZUFB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.098"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..1319)
                     /gene="gltA"
                     /locus_tag="ACHY3M_003754"
     CDS             complement(36..1319)
                     /gene="gltA"
                     /locus_tag="ACHY3M_003754"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFS4318584.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1525..1632)
                     /locus_tag="ACHY3M_003755"
     CDS             complement(1525..1632)
                     /locus_tag="ACHY3M_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318585.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2013..2417
                     /gene="sdhC"
                     /locus_tag="ACHY3M_003756"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2013..2417
                     /gene="sdhC"
                     /locus_tag="ACHY3M_003756"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS4318586.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2411..2758
                     /gene="sdhD"
                     /locus_tag="ACHY3M_003757"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2411..2758
                     /gene="sdhD"
                     /locus_tag="ACHY3M_003757"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFS4318587.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2758..4524
                     /gene="sdhA"
                     /locus_tag="ACHY3M_003758"
     CDS             2758..4524
                     /gene="sdhA"
                     /locus_tag="ACHY3M_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFS4318588.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4540..5256
                     /gene="sdhB"
                     /locus_tag="ACHY3M_003759"
     CDS             4540..5256
                     /gene="sdhB"
                     /locus_tag="ACHY3M_003759"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFS4318589.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5557..8358
                     /gene="sucA"
                     /locus_tag="ACHY3M_003760"
     CDS             5557..8358
                     /gene="sucA"
                     /locus_tag="ACHY3M_003760"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFS4318590.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8373..9590
                     /gene="odhB"
                     /locus_tag="ACHY3M_003761"
     CDS             8373..9590
                     /gene="odhB"
                     /locus_tag="ACHY3M_003761"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFS4318591.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9684..10850
                     /gene="sucC"
                     /locus_tag="ACHY3M_003762"
     CDS             9684..10850
                     /gene="sucC"
                     /locus_tag="ACHY3M_003762"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFS4318592.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGLMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10850..11719
                     /gene="sucD"
                     /locus_tag="ACHY3M_003763"
     CDS             10850..11719
                     /gene="sucD"
                     /locus_tag="ACHY3M_003763"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFS4318593.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11823..12545)
                     /gene="mngR"
                     /locus_tag="ACHY3M_003764"
     CDS             complement(11823..12545)
                     /gene="mngR"
                     /locus_tag="ACHY3M_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFS4318594.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12714..14630
                     /gene="mngA"
                     /locus_tag="ACHY3M_003765"
     CDS             12714..14630
                     /gene="mngA"
                     /locus_tag="ACHY3M_003765"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFS4318595.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     FNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            14648..17281
                     /gene="mngB"
                     /locus_tag="ACHY3M_003766"
     CDS             14648..17281
                     /gene="mngB"
                     /locus_tag="ACHY3M_003766"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFS4318596.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLCVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4321 a         4406 c         4807 g         4240 t
ORIGIN      
        1 gcgggttaaa atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag
       61 tcgcgttttt catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg
      121 tgcatttcgc tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg
      181 aacatggaag acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc
      241 gggtacagtt tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata
      301 gccacttcca gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca
      361 cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg
      421 cggaaagaat catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag
      481 ctaatttctt ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac
      541 agtgaagcaa tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta
      601 cgcacggtgg aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc
      661 atagcacgtt ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata
      721 ttcaggaagt taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg
      781 gaatacttgt aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca
      841 atttcacgat gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg
      901 ccggtaatac cacacatgac tgccattgga tgtgagtcgc gacggaaagc gtggaacaga
      961 cgggtaatct gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac
     1021 tgttcctgag tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa
     1081 tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca
     1141 ataaaagtaa ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac
     1201 acaccttttg aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc
     1261 acatccagtt caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt
     1321 taaggtctcc ttagcgcctt attacgtaag actgccggaa cttaaatttg ccttcgcaca
     1381 tcaacctggc tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct
     1441 gggtacagag ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa
     1501 tcaagtactt agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga
     1561 attggtcaat acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata
     1621 acttttacgc attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt
     1681 caaattagat gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg
     1741 ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg
     1801 ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt
     1861 tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc
     1921 gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt
     1981 gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg
     2041 tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga
     2101 tagcgtccat tctccatcgc gtttccggtg tgatcacctt tattgcagtg ggtatcctgc
     2161 tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta
     2221 tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg
     2281 tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag
     2341 cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag
     2401 gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat
     2461 cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt
     2521 cgccaccagt ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac
     2581 caaagtgttc accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg
     2641 gcaggtgttg accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat
     2701 tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg
     2761 aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc
     2821 gcggcgctgc aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg
     2881 acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat
     2941 gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac
     3001 caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac
     3061 atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt
     3121 cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc
     3181 ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc
     3241 tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc
     3301 gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg
     3361 actggcgggg cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac
     3421 ggtgtcggca tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc
     3481 cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc
     3541 ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa
     3601 gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc
     3661 ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa
     3721 gttctcgaat cccgtctgcc gggtatcctg gagctttccc gtaccttcgc tcacgtcgat
     3781 ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg
     3841 accaaagtta ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt ggttgttccg
     3901 ggactgtttg ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc
     3961 ggcaactcgc tgctggacct ggtggtcttt ggtcgcgcgg caggtctgca tctgcaagag
     4021 tctatcgccg agcagggcgc actgcgcgat gccagcgagt ctgatgttga agtgtctctg
     4081 gatcgcctga accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa
     4141 gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg
     4201 aaagggcttg agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac
     4261 acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa
     4321 acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc
     4381 cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca
     4441 gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc
     4501 ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt
     4561 tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg
     4621 gaagaaggtc gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc
     4681 agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg
     4741 aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag
     4801 aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg
     4861 ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat
     4921 ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat
     4981 gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat
     5041 aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat
     5101 accgagactg acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac
     5161 agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc
     5221 catattaagt cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca
     5281 agcgtcgcat caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt
     5341 cattacccgt aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc
     5401 tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat
     5461 agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg
     5521 cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc
     5581 tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa
     5641 gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta
     5701 cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc
     5761 ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag
     5821 caggttaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat
     5881 ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac
     5941 gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa
     6001 gaaaccatga aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt
     6061 ggtgccgagt atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc
     6121 gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc
     6181 gccgctgaag gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg
     6241 ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac
     6301 agcggcaccc gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg
     6361 aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa
     6421 cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat
     6481 ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg
     6541 gtagttatcg gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa
     6601 gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa
     6661 actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc
     6721 aacaaccagg ttggtttcac cacctctaat ccgctggatg ctcgttctac accgtactgt
     6781 actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa
     6841 gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc
     6901 ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca
     6961 acccagccgc tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct
     7021 gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg
     7081 taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg
     7141 cactctttca cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa
     7201 gttgagatga agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt
     7261 gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag
     7321 aaactgttcg actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc
     7381 attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg
     7441 gtgatccaca accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg
     7501 cagggcgcgt tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa
     7561 tacggttatg ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac
     7621 ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg
     7681 ggccggatgt gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag
     7741 cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtg
     7801 tgcgtaccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg
     7861 atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt
     7921 tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag
     7981 cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg
     8041 ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc
     8101 tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat
     8161 tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat
     8221 ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc
     8281 tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac
     8341 gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg
     8401 acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg
     8461 cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac
     8521 cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt
     8581 ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca
     8641 aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcatctctg gaagagcaaa
     8701 acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca
     8761 gcgccattaa aggcaccggt gtgggggggc gtctgactcg tgaagatgtg gaaaaacatc
     8821 tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg
     8881 ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag
     8941 agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca
     9001 tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca
     9061 tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc
     9121 cggaagtgaa cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca
     9181 gcatggcggt ttctacgccg cgcggcctgg tgacaccggt actgcgtgat gtcgataccc
     9241 tcggcatggc agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca
     9301 agctgaccgt tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg
     9361 gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg
     9421 ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc
     9481 tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa
     9541 cgatcaaaga gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc
     9601 ttcacctgca ctgtagaccg acatggatag ggtgtcggtc tacggtttaa aagataacga
     9661 ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg
     9721 cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag
     9781 aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg
     9841 gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg
     9901 cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg
     9961 ttaaccagat tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg
    10021 ttgtagaccg tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa
    10081 tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga
    10141 ctggcctgat gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac
    10201 tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc
    10261 tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg
    10321 acggcaaact gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc
    10381 gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg
    10441 ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga
    10501 tggacatcgt taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg
    10561 caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag
    10621 ccgttctggt taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca
    10681 tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca
    10741 acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag
    10801 gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt
    10861 aattgataaa aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca
    10921 ctcagaacag gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg
    10981 cggtactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac
    11041 tggcgctacc gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga
    11101 agctattgat gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga
    11161 tatgctgact gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg
    11221 cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa
    11281 accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca
    11341 gaccacggat tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc
    11401 tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat
    11461 cgtaatgatc ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga
    11521 gcatgttacc aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg
    11581 tatgggccac gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc
    11641 tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact
    11701 gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca
    11761 ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt
    11821 gcttatcgcg atgattttcg ttgcgctatc agggtaaatt tatagtcatc ggtattaaaa
    11881 gcgttgcggc tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca
    11941 agaatcggct ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga
    12001 ggaatgattt cctgttcgct acgatcgata accattttct tcacttcttc aataaagtga
    12061 tattttgaat tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag
    12121 gtttcttcca gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca
    12181 cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca
    12241 aatatcagga cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg
    12301 gttaactgaa aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga
    12361 atgctttcga ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg
    12421 ctgacgccaa actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc
    12481 tcgccacgcg caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt
    12541 cccattttta gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt
    12601 aaataatata aatacaatac aaataatttc aatcaagtga aattgatcac ataatggtat
    12661 tgttttatcg ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc
    12721 tgacgactct gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag
    12781 aggccatcca cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc
    12841 aatttctgga agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt
    12901 tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac
    12961 tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga agcggttgat ttagtggtgc
    13021 tgctggcgat tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga
    13081 ccacgcgcct ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg
    13141 agttgctctc ggcgctggat gacaagggag gcacgcaacc ttctgcctct tttttcaacg
    13201 cgccaactat cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg
    13261 cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag
    13321 gcgctaacgg cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta
    13381 tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt
    13441 cagtgcctgt tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc
    13501 tcaagcgtag cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca
    13561 aaacggagct gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg
    13621 cggggggcac ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc
    13681 tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg
    13741 gaattttgat ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg
    13801 cgttagcgcc aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg
    13861 gcgcggtcgt tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc
    13921 gtcttggcag taaattcaat ggcttcctga ctttttatct ctacccggtg ctcggtacgt
    13981 tgggagcggg cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact
    14041 cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc
    14101 tcggttttat gtgttccttt gaccttggag ggccagtgaa taaagccgct tatgcattct
    14161 gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa
    14221 tggtttcggc atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt
    14281 ttgaaattga gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg
    14341 cgatcccgat ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta
    14401 tggtaacggg cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca
    14461 ttttctcgct ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct
    14521 ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc aattctcctg atgtggcggc
    14581 gtcacgcggt taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga
    14641 aacgacgatg aaagcggtat ctcgcgttca catcaccccg catatgcact gggatcggga
    14701 gtggtatttc accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct
    14761 gtgccgactg gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat
    14821 cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac cgtgtgaaga aacaggtaga
    14881 agccggcaag ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc
    14941 ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc
    15001 gatgaaaata ggttatttac cggattcctt tggcatgtcc gggcagcttc cgcatatcta
    15061 caatggattt ggcattaccc gcaccatgtt ctggcgtgga tgttctgagc gccacggtac
    15121 tgataaaacc gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaagtgtt
    15181 gccgctgggc tacgccatcg gtaagtactt acctgccgac gaagacggat tacgcaagcg
    15241 cctcgacagt tattttgatg tgctggaaaa agcgtcagta accaaagaaa ttttattgcc
    15301 gaatggtcat gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg
    15361 tgagatctac cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat
    15421 cgaagcgcag cgagataatc tggcaaccct gaaaggggaa tttattgatg gcaaatatat
    15481 gcgcgtgcat cgcaccatcg gttctacgcg tatggatatc aaaattgccc acgcgcgtat
    15541 tgaaaataag attgttaatc tgctggaacc gctggcaaca ctggcctgga cgttgggttt
    15601 tgaataccac cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca
    15661 cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc gaaatcgtcg cccgcttcga
    15721 actggctgaa gacatggcgg ataatctgat tcgtttctac atgcgcaaaa ttgccgacaa
    15781 catgccgcag agcgacgccg acaaactcgt cctgtttaac ctgatgccct ggccgcgtga
    15841 agaagtgatc aacaccactg tgcgcctgcg cgccagccag tttaatttgc gggacgatcg
    15901 cggccagcct gtgccgtatt ttattcgcca tgcccgtgag atcgatccag gcctaatcga
    15961 tcggcaaatt gttcattatg gcaattatga tccctttatg gagttcgaca tccagatcaa
    16021 ccagattgtc ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa
    16081 cgtgattgcg gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc
    16141 gctcaatgag gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg
    16201 ggtattgcaa attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa
    16261 agaagagtgg gtaattaccg cggcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc
    16321 ctggcagagc agggctgtta tccgttatga aatggcggtg ccgcgtaatc tgcaagaacg
    16381 cagtgcaaga caatccactg gcagagtagg ggtggagatg gttgtcactc tgagtcataa
    16441 cagcagacgt attgatgtgg atatcaacct tgataaccag gctgacgatc atcgcctttg
    16501 cgtcctgatc cctacacctt ttaacaccga cagtgttctg gcagatacgc agtttggttc
    16561 gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga
    16621 agcgccggtt ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg
    16681 catggctgtc tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac
    16741 ctttgccatt acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag
    16801 gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct
    16861 ttcttgtcgc ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca
    16921 gcaggcgcga gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat
    16981 gaagctcaac aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc
    17041 agtgggatgc ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg
    17101 gctgtttaat ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt
    17161 gatttcttgc tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc
    17221 aaatctatcg gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg
    17281 aatagcaatc aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt
    17341 tcatgccgga tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata
    17401 ttgcagagat gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt
    17461 cactctcaag ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt
    17521 aattataata taattataag ttaattatat gttaatatca gcggggtgga tttatgcctt
    17581 tattagtaat cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc
    17641 aaccgtgctg tttttgcttc gtctcttttt atctttaatt gccaaccgaa actaatttca
    17701 gccttataac tcacacattt taaacataaa tgtcactaaa gttaccttat tgaaacatga
    17761 ttaacataat ttgt
//
LOCUS       DAZUFB010000099        17559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZUFB010000099 DAZUFB010000000
VERSION     DAZUFB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.099"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..1154)
                     /locus_tag="ACHY3M_003767"
     CDS             complement(153..1154)
                     /locus_tag="ACHY3M_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4318597.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1261..1557
                     /locus_tag="ACHY3M_003768"
     CDS             1261..1557
                     /locus_tag="ACHY3M_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4318598.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1691..2116)
                     /gene="arsC"
                     /locus_tag="ACHY3M_003769"
     CDS             complement(1691..2116)
                     /gene="arsC"
                     /locus_tag="ACHY3M_003769"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFS4318599.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAPKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2129..3418)
                     /gene="arsB"
                     /locus_tag="ACHY3M_003770"
     CDS             complement(2129..3418)
                     /gene="arsB"
                     /locus_tag="ACHY3M_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFS4318600.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3472..3825)
                     /gene="arsR"
                     /locus_tag="ACHY3M_003771"
     CDS             complement(3472..3825)
                     /gene="arsR"
                     /locus_tag="ACHY3M_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFS4318601.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4334..4417
                     /gene="dinQ"
                     /locus_tag="ACHY3M_003772"
     CDS             4334..4417
                     /gene="dinQ"
                     /locus_tag="ACHY3M_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFS4318602.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4471..5823)
                     /gene="gorA"
                     /locus_tag="ACHY3M_003773"
     CDS             complement(4471..5823)
                     /gene="gorA"
                     /locus_tag="ACHY3M_003773"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4318603.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5895..6737)
                     /gene="rlmJ"
                     /locus_tag="ACHY3M_003774"
     CDS             complement(5895..6737)
                     /gene="rlmJ"
                     /locus_tag="ACHY3M_003774"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFS4318604.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6940..8982
                     /gene="prlC"
                     /locus_tag="ACHY3M_003775"
     CDS             6940..8982
                     /gene="prlC"
                     /locus_tag="ACHY3M_003775"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFS4318605.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYNAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8990..9742
                     /gene="rsmJ"
                     /locus_tag="ACHY3M_003776"
     CDS             8990..9742
                     /gene="rsmJ"
                     /locus_tag="ACHY3M_003776"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFS4318606.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9791..11260)
                     /gene="dtpB"
                     /locus_tag="ACHY3M_003777"
     CDS             complement(9791..11260)
                     /gene="dtpB"
                     /locus_tag="ACHY3M_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFS4318607.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11426..11479
                     /locus_tag="ACHY3M_003778"
     CDS             11426..11479
                     /locus_tag="ACHY3M_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318608.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11578..12012)
                     /gene="uspA"
                     /locus_tag="ACHY3M_003779"
     CDS             complement(11578..12012)
                     /gene="uspA"
                     /locus_tag="ACHY3M_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFS4318609.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12403..12738
                     /gene="uspB"
                     /locus_tag="ACHY3M_003780"
     CDS             12403..12738
                     /gene="uspB"
                     /locus_tag="ACHY3M_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFS4318610.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12983..14482)
                     /gene="pitA"
                     /locus_tag="ACHY3M_003781"
     CDS             complement(12983..14482)
                     /gene="pitA"
                     /locus_tag="ACHY3M_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFS4318611.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPSVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14714..15916
                     /gene="yhiN"
                     /locus_tag="ACHY3M_003782"
     CDS             14714..15916
                     /gene="yhiN"
                     /locus_tag="ACHY3M_003782"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4318612.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16232..17284)
                     /gene="yhiM"
                     /locus_tag="ACHY3M_003783"
     CDS             complement(16232..17284)
                     /gene="yhiM"
                     /locus_tag="ACHY3M_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFS4318613.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVASHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4571 a         4425 c         4494 g         4069 t
ORIGIN      
        1 ttgtctgttg atgacttgtt gaccgagccg tcatcattct caatgcatta gttaaatcat
       61 ttgtgatttt taaggattat cctgtctatg attttctact tagaaggata tggcactaac
      121 tatgcatctt atagtttact gctggcatct atttaaaata gcaattgagt caaatagccc
      181 atcaagatag ccatacctaa tataaccgtc aggaatgcag ctatcatcgg cattctgaac
      241 attgatttaa gtaatatcac ttccgtcagg cttgcgccgg cactgccgat gattaatgcc
      301 attaatgctc ccagacccat tccttttgtc atcagaacag atgccagagg gataactgcc
      361 tcagcccgga tatacagcgg aataccgaca acggcgctta atgggatggc gaagggatta
      421 tctgctcctg catgagcggc aatccacgct gagggaataa agccataaat aaaagatcct
      481 attaatactc ctaacaaaag atatggcagt acatctttaa actgttgcaa cgcatctttg
      541 gtggctagtt ttatgggatt aatacttact acagctttgg tactgcaaca ggtaactacg
      601 gggttctcaa tggctttagc cgctggacta caacaactca cttttatggg cgtatatgtc
      661 gttcccggcg aagtttttgc tggagcacaa caatttgctg atgagctttt actggcaatg
      721 atatgacgtt caaaccccag agaatccagg ataatactgg caagaacgga gacgccggct
      781 gcgataatcg cgtacaacaa ggtgactttc caaccaaagg tcatccacat tagtccgacg
      841 ataatgggat taagtaatgg tgaaacaaat aaaaaagtga gagtcggacc aaacccggct
      901 ttcgctgaca acaatccacg taacatgggt attgtcgagc aactacagaa cggggtaacg
      961 gctcccaaca gagcagccag gagatagcct ctcccttttc tggcccccat catctgctgg
     1021 attttatggt ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac
     1081 agcaacgaaa gctcgacggc gagaaacaca aacatctctg cggcatcttg cagcatcgca
     1141 agccaactac tcatacgtaa cctcattgga taaatctata tttccagaat aatcgaaatg
     1201 aaattcagat caagatattt ctggtattat cgaaatatca accagacaga ggggtgtcat
     1261 atgcagttgg aagaagtcgc taaagcgctg aaagagctag gtcacccgac ccgtttgttc
     1321 atatttaaac atcttgtcaa agccggggaa caagggttgc cggtcggtga gttgcaaaag
     1381 cagcttggga tacctggctc cacactcagc caccatatat cagcactggt atcggtagga
     1441 ctggtcaaac aaaaccgtga gagtcggacg ctgatgtgtg tttcgcaata cgcaatgctg
     1501 gaagcgataa ttgaattctt acgcgaggag tgctgcgtaa atagcaaaat tgcctgatgc
     1561 gctacgctct acatatttca tgccatatat tgaatttgca cggttttgta ggtcggataa
     1621 gacgcgttag cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga
     1681 tatcgccgct ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct
     1741 tggagaatgc gccttttggc gcatctggca gaatttccag caccacttct gaagggcggc
     1801 acaggcgagt tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa
     1861 gcataaagtc gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat
     1921 acggttcgac gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt
     1981 tgaccagttc atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc
     2041 cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa
     2101 tggtaatgtt gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg
     2161 cagccagcgt cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc
     2221 cccagctgat agtcatattc ttctgcgaaa gtacgtgcag ccagagtagc gtagccaggc
     2281 taccaattgg ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg
     2341 cttctttgat aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg
     2401 tcggcatatt gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg
     2461 cggcccacag gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc
     2521 cggcattgcg caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc
     2581 agggggcacc gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa
     2641 ataatatcag cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga
     2701 cgaaaaatcc caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat
     2761 ctttgatcgc ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt
     2821 tgcgaaaata gagatgtaac atcaccagcg tggcaacaat cgcggcgata tccaccggca
     2881 ccatcaccga ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca
     2941 ccaagttgga gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca
     3001 cgaacgccag cgtagtgcct ttactgaacc ctaaagccag cagcatggcg atgacaatcg
     3061 gtgtcaaaat aagcgccgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca
     3121 ggacaatcca ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg
     3181 ccgcccattc aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa
     3241 acgcagccgt cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca
     3301 cgcccgtaac taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt
     3361 tcggctgcca gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa
     3421 tgcctcccgg ataaaacaca tctgaaaatt catatgtgtt tagctaaatt tttaactgca
     3481 aatgttctta ctgtccccgg aacagttttg tcgagccagg ttgcggacaa tcgcctgaac
     3541 cttttcctgt tcacatcgcc aggcctcatc aataattttc gccgcccatg ctggaatatg
     3601 cggtgataag cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc
     3661 acgcagcaat gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag
     3721 atcgcagacg cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc
     3781 atcagcaaga attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt
     3841 gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat
     3901 gtgtaagtgc agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga
     3961 ggtctgaatc aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga
     4021 gttggtggca ctggctggag tgagaatgcc attatctaac gcatcaatgc taataccata
     4081 ctgaaactat tgcaaggaca tgctggtttt ataacctgaa tgtactgtat gattatccag
     4141 ttagctctga ggcattttca ctctggtaat gcgcataaac gctttcaaag tcctggtcag
     4201 aagtacgggt ggtgccgtta actgatgctc tggccggagt gagaatgcca ttatctaaca
     4261 atagggcatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc
     4321 atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa ttgcgctgct
     4381 ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct
     4441 aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgccgc
     4501 cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgcaccca tcttcagcgc
     4561 caccgcgaag ccctgcaaca tttcgtccat accaaagcca atgccgtgaa taccgacaat
     4621 cttctcttcc gatccaacgc acaccagctt catgcggcac ggctggcggt gagtggtgac
     4681 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccatactg
     4741 ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
     4801 ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga
     4861 gagacggcga cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc
     4921 caccgcgtaa ataccttcaa tattggtgtt ttgatattta tcgacgacga tatagccttt
     4981 ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
     5041 aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag
     5101 gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc
     5161 ttcggcgttc atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg
     5221 cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag
     5281 ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc
     5341 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
     5401 accgcctgtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt
     5461 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
     5521 gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc
     5581 ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca tatggatcgc
     5641 ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa catttacgca
     5701 ggtgccgccc agctctttgg cttcaatcag cgcacatttc tggccgtaca tagccgcgcg
     5761 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
     5821 catgacaaag tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
     5881 agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc
     5941 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc cagtttccac
     6001 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt
     6061 accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc gtggatcatg
     6121 cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc agtggcgaaa
     6181 cgtttgtaac cttctgctat cccgctgacc accgcctgat agtcggtttt catttcatac
     6241 ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc
     6301 tggaaaccat cggctttttc gacgcgcgca cggctgtctt tctgaaattc agaacgcagc
     6361 aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc
     6421 tggcgagcaa tcaatggcga accggggtag taacgcaact gaccgctacg gttgaagtgt
     6481 tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg ccagatacgg
     6541 gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
     6601 ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg
     6661 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
     6721 tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg
     6781 gcgcacaaaa gcgtatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
     6841 gtttctcatt gaaattcact acacttaacc ccatgctaca cacattatgt aaagcgcctg
     6901 ttgagcgctt ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc gttactgact
     6961 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
     7021 aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg agcaccgtac
     7081 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg gcgtatcttc
     7141 tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa
     7201 caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga agggctgtat
     7261 aacgcgtatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggctcagaaa
     7321 aaagcggttg ataacgcact gcgcgacttc gaactctctg gcatcggtct gccaatagag
     7381 aaacaacaac gctacggtga gattgccact cgtctttccg agctgggcaa ccagtacagc
     7441 aacaacgtcc tcgatgcgac gatgggctgg accaaactcg ttaccgacga agcggagctg
     7501 gcggggatgc ctgaaagcgc gctggctgcg gcaaaagccc aggccgaagc gaaagagctg
     7561 gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
     7621 aaccaggcct tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc
     7681 ccgaacgccg gtaaatggga taacagcaag gtgatggaag agatcctcgc tctgcgtcac
     7741 gaactggcgc aactgctggg ctttgaaaac tacgccttta aatcccttgc cactaaaatg
     7801 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg
     7861 caaggcgaaa aagagctggc acaactgcgc gccttcgcca aagcggaatt tggcgtcgat
     7921 gagttgcagc cgtgggatat cgcgtactac agcgaaaaac aaaaacagca cctctacagc
     7981 atcagcgacg agcagctgcg tccgtacttc ccagaaaaca aagcggttaa cggcctgttt
     8041 gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
     8101 catccggatg tacgtttctt cgaactgtat gacgaaaata acgaactgcg cggcagcttc
     8161 tacctcgatc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta
     8221 ggccagatgc gtaaagctga tggttcgctg caaaaaccgg tcgcgtattt gacttgtaac
     8281 ttcaaccgcc cggtaaatgg taaaccggcg ctgttcactc acgacgaagt gatcaccctg
     8341 ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt
     8401 tccggtatca gcggtgtgcc gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac
     8461 tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac cggcgaaccg
     8521 ctgccgaaag agttgctgga taaaatgctg gcggcgaaga attaccaggc ggcgctgttt
     8581 attctgcgtc agctggagtt cggcctgttt gatttccgcc ttcatgccga gttccgcccg
     8641 gatcaggggg caaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtggtg
     8701 ccatctccgt cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat
     8761 gcggcaggtt actacagcta cctgtgggct gacgtactgg cggcagatgc tttctcgcgc
     8821 tttgaggaag agggcatttt caaccgtgaa accgggcagt cgttcctcga caacattctg
     8881 agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg
     8941 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
     9001 cttaattgat gaaacaggcg ccggagacgg cgccttatct gttctggcgg cccgctgggg
     9061 gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac atctggaatt
     9121 gcgcaagcgt gatgagccaa aacttggcgg catatttgtt gattttgttg gcggagcgat
     9181 ggcgcaccga cgcaaattcg gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat
     9241 taaaggcgat tatttgccgg atgtggtgga cgccaccgca gggctggggc gcgatgcctt
     9301 tgtgctggct tcagtcggtt gccgcgtgcg gatgctggag cgtaatccag tcgttgccgc
     9361 attgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca
     9421 ggagcgtttg cagttaattc acgcctccag cctgacggcg ctaaccgata ttaccccacg
     9481 cccgcaggtg gtctatctcg acccgatgtt cccgcataag cagaaaagcg cgctggtgaa
     9541 aaaagagatg cgtgtctttc agtcgctggt ggggccggat cttgatgccg atggattgct
     9601 ggagcctgca cgcttgctgg cgaccaaacg cgtggtagta aagcgcccgg actacgcgcc
     9661 accgctagcg aatgtcgcca cgccaaacgc ggttgtgact aaagggcatc gctttgatat
     9721 ctatgcaggt acgcccgtgt aaaagtaaag cagggtagcg ataacgctac cctgctttct
     9781 gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac ggcaccatca
     9841 acagcatcac tactgcaacg cccagcgtga ccagaccaat cttaccaaac acgttggtat
     9901 agacgggcaa cgtctcaagc ggatcggtaa tgttgtccgg caccgcggta aatgttgcca
     9961 catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg agaataaagc
    10021 ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg ctaataaaca
    10081 gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat gtcagccctt
    10141 gtgcatccgc aaaccacatt cccgcagctg ccgccgtcaa aaagcccagt gagcacataa
    10201 acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta cccagatgcg
    10261 tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta agcgcctgga
    10321 agctgaccgg gttgatggaa aaaccgagaa tttcatgatg cacgttgttg atggcaaaga
    10381 agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca
    10441 ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat gcctgacgaa
    10501 agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg acttctacgt
    10561 tgtgcatcag ccatgcacat acgaagatca tcaccacgct gccaagtaat acgtacaaca
    10621 gtttgctgaa gctcatcggc ttgaagtcgg gttcagaacc aatgtctttc accattccac
    10681 gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga
    10741 ctgaataacc gaatctatca gcgatcacag gggccagcga taacgctatc aacgagccga
    10801 tattgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct ttcggcggat
    10861 agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg acagcgatag
    10921 tccccagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc atgaagtagc
    10981 caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc
    11041 cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca ccaaaagtga
    11101 caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc agtacgccct
    11161 gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg aaaaatgggc
    11221 gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac cttccaatac
    11281 catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag
    11341 attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa ataaccagct
    11401 gatcacacta atgatggaaa atatgatggt gttaaatgtt gtattggcag gagattttga
    11461 ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta acaaggcgcg
    11521 ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga gggatgatta
    11581 ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg
    11641 acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc
    11701 catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc tgccgctcag
    11761 ggtttcagtg attgggtagc ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc
    11821 ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg tgtataggtc
    11881 agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga
    11941 gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga tgagaatgtg
    12001 tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt
    12061 cttgtgccgt tacgtcaccc gcgtcctgcg aactatgtgc gcggggctaa cccagccgta
    12121 aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc
    12181 ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc
    12241 tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc
    12301 acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag
    12361 ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca
    12421 ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta
    12481 cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga
    12541 gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc
    12601 tatgcccaac gttatcgcga tcatcacgat gatgaattta ttcgtcgctg tgagcgggtg
    12661 cgtcggcagt ttattctgac cagcgcattg tgtggtctgg tggtggtcag cctgattgca
    12721 ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc gggccagtaa
    12781 actgacccgc tcgagactga ttacaaacgc aaaattgcct gatgcgctac gcttatcagt
    12841 atctacataa tctctgcaat ctattgaatt tgcatgtttt tgtaggccgg ataaggcgtt
    12901 cacgccgcat ccggcatgaa caaagcgcac tatgtcacaa tctgaagcgg gccagctgac
    12961 ccgctcgttt tggtgtgtac gattacagga actgcaagga gagccagtac agtccgccgg
    13021 aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatactg gttacggttt
    13081 tacgctgtaa gccgccgcca tctaccacca tcgtccccgc gacagaagag gagagtacgt
    13141 gagtagtgga aaccggcatc ccggtataac tcgccaggcc gatagacact gccgccgtca
    13201 tctgggccga catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag
    13261 tcgttgccac acggcgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca
    13321 tgatgatcca caccggtgca tactcgatgg tgctaagcat gtcggacttc agtttcttca
    13381 acaggcgctg atcgtcagca ctcacactag gcatcttcac cactttgtcg atagtgtcag
    13441 aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc
    13501 tttccacatc ggtagtcaac atgcctttca ggcggttgag cgcgttaatg gtattcgacg
    13561 gatggcaatg gaactccgcg ggttgcgttg cgccagcttc cggagccggt actaactgat
    13621 cagcaccggt agcctgtttg agcagcgcag gatgctgctc aaagtaagct tcgacgttgt
    13681 tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc
    13741 ctgctggtgc gacgccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt
    13801 tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcaata cgcgtccaga
    13861 acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg
    13921 cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca
    13981 ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat
    14041 tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga
    14101 tcgcgccaat cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt
    14161 tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca
    14221 tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac
    14281 ccagcaaaac acccaaaaag ttgaacaccg ctgccataac cacggcgagc tgagaacgca
    14341 tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg
    14401 cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca
    14461 ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca
    14521 ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc
    14581 tgtatggatt tcactcaaaa aataattatc ttatataatt caggcaaata cttcctttta
    14641 gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc gttttttcta
    14701 agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat
    14761 gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa
    14821 aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta
    14881 tgtcgaacca ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg
    14941 ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa
    15001 aacgttagga caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga
    15061 tgagtgcgag aagggcaatg taaccttcag attgcgtagc gaagtgctga gtgtggcgaa
    15121 ggatgaaaca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt
    15181 catcgcgacc ggtgggctgt caatgccggg gctgggcgcg tcgccgtttg gttataagat
    15241 tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct
    15301 gcataaaccg ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat
    15361 taccgctgaa aacggcatcg ttttccgtga gaacttactc ttcacccatc gcggcttgtc
    15421 tggaccggcg gtgttgcaga tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa
    15481 tctgctaccg gatgtggatc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa
    15541 tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca
    15601 gcaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc
    15661 actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac
    15721 tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc
    15781 gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg gacgttaccg gctggctggg
    15841 gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc
    15901 agcgaagtcg tcctgacctg ttacctaaag atgaaaatta ttagagccgc attaaaaatg
    15961 agctaatttt gatagtggct atcttgtgat tattttctaa tgagcccgtg aactgaaacc
    16021 ctccaggctt aatataaggt ggaaggaaag gtgattgaaa actcacccag tggccagatc
    16081 tttataaaat atgaacaggt tgaaaaaaac agtaactttg ttgttttttt atccttaacc
    16141 actatgcatt aatgctgcgt tatttcatga tgcctaagaa aaaccagcgt tacgcaaatg
    16201 gtcaacgctg gttttatccg gtacgttgca attatttttt agcagaacct gcttctaata
    16261 ttgaaacgat tgagaacaac gtaaagcata ccgctcctaa accgaccaaa acccgcgaag
    16321 gaataaaata tcccatgtcg gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc
    16381 aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc
    16441 tccataccag tgccagtagc cagacttttg agaatatgct gtaacagatc attccgaggc
    16501 aaataagaat aatgccggga gccagtcggg cgcttgcatc ggaactgact aagacgtata
    16561 tcccctgcag taccgtgatt gagccgagaa aaataaccca ataacaccac agccagtgtt
    16621 ctttagttga aaacgtatta cgtgtttgat ggacaattgt ggcaacaagg ccaattagac
    16681 aggcacagat tgcggttaac ccaagcaata cgtgactcgc gacataatgc ggagttatgt
    16741 cagcactacg taacagcgta atagaccaaa tgaaaccgag cagggtgagt aaaacgggaa
    16801 cggcaatgag gcaattaccg attaatgaag aataagcctg taccggcgtt ccatcgctct
    16861 tgctccccgc tgcatttttg ggaatgagca gaaagtgacc ggatgacgct gccaccgtcg
    16921 atacacaggc ggcaatcata ccaacgccga aaataacatg gccggcgaca aactcgtctg
    16981 ccatcacatc attgcctgct aacagtgccc aaccccatat catggtgata attgaccccg
    17041 catagccaat aatggggaac aatgtattgt aaaacgtatt aacgccgcgc gtgagctgcg
    17101 aaatgataat aaatgcggta gtgaataatg ccaggcatat ggctgccaga gaaatcagca
    17161 catgacccgc aacgaaatac tcactttgcc cagaactttc cagcacaaag ccacctaacg
    17221 cgatacaaat taagcccata attaaaggga ttaatttgaa aagccacccg atatatatgt
    17281 tcactttaaa ctcctcaggt atccctgcta atattgaaat gtgcattaat gataatttca
    17341 gcctgttaat ataatgacga gatatcatta aagacaaatg catagcataa ctatagcggg
    17401 atttactttg cagagcgcta cttttcagcg ttcaaaaggc catggtaaat aaatatttat
    17461 cctaagatta attcttattc tgttgttttt tacaattatc ttccagggag ggattctttt
    17521 tcattatttg gtaaaagacc ggatggtatc ggtgcttaa
//
LOCUS       DAZUFB010000100        17362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZUFB010000100 DAZUFB010000000
VERSION     DAZUFB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.100"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..826)
                     /gene="rlmB"
                     /locus_tag="ACHY3M_003784"
     CDS             complement(95..826)
                     /gene="rlmB"
                     /locus_tag="ACHY3M_003784"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4318614.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELICIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1006..3366)
                     /gene="rnr"
                     /locus_tag="ACHY3M_003785"
     CDS             complement(1006..3366)
                     /gene="rnr"
                     /locus_tag="ACHY3M_003785"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4318615.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKKVAE"
     gene            complement(3405..3830)
                     /gene="nsrR"
                     /locus_tag="ACHY3M_003786"
     CDS             complement(3405..3830)
                     /gene="nsrR"
                     /locus_tag="ACHY3M_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFS4318616.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4035..5333)
                     /gene="purA"
                     /locus_tag="ACHY3M_003787"
     CDS             complement(4035..5333)
                     /gene="purA"
                     /locus_tag="ACHY3M_003787"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4318617.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5437..5634)
                     /gene="yjeT"
                     /locus_tag="ACHY3M_003788"
     CDS             complement(5437..5634)
                     /gene="yjeT"
                     /locus_tag="ACHY3M_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFS4318618.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5716..6720)
                     /gene="hflC"
                     /locus_tag="ACHY3M_003789"
     CDS             complement(5716..6720)
                     /gene="hflC"
                     /locus_tag="ACHY3M_003789"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFS4318619.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6723..7982)
                     /gene="hflK"
                     /locus_tag="ACHY3M_003790"
     CDS             complement(6723..7982)
                     /gene="hflK"
                     /locus_tag="ACHY3M_003790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFS4318620.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8068..9348)
                     /gene="hflX"
                     /locus_tag="ACHY3M_003791"
     CDS             complement(8068..9348)
                     /gene="hflX"
                     /locus_tag="ACHY3M_003791"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFS4318621.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGALVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9425..9733)
                     /gene="hfq"
                     /locus_tag="ACHY3M_003792"
     CDS             complement(9425..9733)
                     /gene="hfq"
                     /locus_tag="ACHY3M_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFS4318622.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9819..10769)
                     /gene="miaA"
                     /locus_tag="ACHY3M_003793"
     CDS             complement(9819..10769)
                     /gene="miaA"
                     /locus_tag="ACHY3M_003793"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4318623.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10762..12609)
                     /gene="mutL"
                     /locus_tag="ACHY3M_003794"
     CDS             complement(10762..12609)
                     /gene="mutL"
                     /locus_tag="ACHY3M_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4318624.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12619..13953)
                     /gene="amiB"
                     /locus_tag="ACHY3M_003795"
     CDS             complement(12619..13953)
                     /gene="amiB"
                     /locus_tag="ACHY3M_003795"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFS4318625.1"
                     /translation="MMYRIRNWLVATLLLCTPVGAATLSDIQVSNGNQQARITLSFIG
                     DPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLT
                     ENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNPF
                     KTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVT
                     IAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRS
                     ATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFG
                     HSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEER
                     LLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13972..14433)
                     /gene="tsaE"
                     /locus_tag="ACHY3M_003796"
     CDS             complement(13972..14433)
                     /gene="tsaE"
                     /locus_tag="ACHY3M_003796"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4318626.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14405..15952)
                     /gene="nnr"
                     /locus_tag="ACHY3M_003797"
     CDS             complement(14405..15952)
                     /gene="nnr"
                     /locus_tag="ACHY3M_003797"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFS4318627.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15951..17090
                     /gene="queG"
                     /locus_tag="ACHY3M_003798"
     CDS             15951..17090
                     /gene="queG"
                     /locus_tag="ACHY3M_003798"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4318628.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFLQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17073..17126)
                     /locus_tag="ACHY3M_003799"
     CDS             complement(17073..17126)
                     /locus_tag="ACHY3M_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318629.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3957 a         4952 c         4355 g         4098 t
ORIGIN      
        1 aataaaatga atatttgcag tacgtaatta tcttaccagc tatagacaaa aaaaaaccat
       61 ccaaatctgg atggcttttc ataattcaga cgaactagct gcgctgacga accgcttcaa
      121 ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct gccattggga
      181 tgcagatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca ccttccgcgc
      241 ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga tccgcctcgc
      301 ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg cgcgcaaggt
      361 tagtcacccg aatcagtgga acgctttctg ctgcgccgca ggctactttt ttcgccgtag
      421 cgttgagctg tgcggagcga tctttcggca caatcaccgc gtgaacgcca gcagcgtccg
      481 cgctacgcag gcacgcgccg aggttgtgcg gatcggttac accgtcgagg atcagcagga
      541 acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac tgacgtcctg
      601 gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg tcgagatatt
      661 ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc agcggtaaca
      721 gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca ggggcgcgct
      781 ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat gtactcgttg
      841 tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc aatatattga
      901 atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg
      961 cattttgtca gcaatctaac cctcttcttt taaagagggt attgatcact ctgccacttt
     1021 tttcttcgcc gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa
     1081 gtttaccttt ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt
     1141 cgctttctcg cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact
     1201 aaagtcgatt ttgcgctcgt ccatattaac cgcttcgact cgaacttcca cgcgatcgcc
     1261 caggcgataa gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg
     1321 atagtagtca ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag
     1381 acggacgaag aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac
     1441 ctggtcgagc atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc
     1501 acgacgttcc gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg
     1561 gtagccgccg gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatattt
     1621 aatggcgcgg tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata
     1681 ggactgcaat gccagaccga agtgaccacg gttttctgga tcgtaaatcg cctgtttcat
     1741 cgagcgtagc agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag
     1801 cagttccgcg tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc
     1861 cgccagcact gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa
     1921 cagtgccggt tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat
     1981 gcactcttca attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg
     2041 gcgttcagcg ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc
     2101 ttcacgggct ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag
     2161 cggggcgtac tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta
     2221 ggtcagacgc gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc
     2281 tttcgacgaa accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga
     2341 acacaggccg ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac
     2401 cgacgtgcca cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct
     2461 gacgtcggca atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac
     2521 tgcatcgtca aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcag
     2581 atcaacacgg cccgcttttg cttcttccgg cacttcttct ttcagcccgg caacctgttg
     2641 ctcaacagcc tgcggccaga tgtacggaat ttcatgggta cgcagagcga tatcaaccgc
     2701 catgccggtg cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg
     2761 gcgagtcgga cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat
     2821 ctgatcgggc gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc
     2881 gacgcccgct tcagtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg
     2941 gacaatacgc gcttcacgac gacctttacg gtcagcgccc agcggctgcg ccagcacctg
     3001 atcgccatga atgcaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc
     3061 ttcaacccgc agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc
     3121 gaggcgttcc ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc
     3181 catcgcgcgc aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc
     3241 taccgccagc tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa
     3301 ttcccgacta gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg
     3361 tgacatctcg gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat
     3421 aatttataaa gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc
     3481 gtaagaaaac tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg
     3541 tggcaaaact cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca
     3601 ccaatacgta tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca
     3661 gcagtcacgt agccggcacg actaagttga ttgattattt tgaccatatg attacgggag
     3721 acgccgtaga cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc
     3781 atgtagatca gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca
     3841 aaagggaaat cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg
     3901 ctaatttatt tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg
     3961 taatggctta accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc
     4021 caggcgtacc agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc
     4081 ggaccggtag agatgatatc gatcggcaca ccagtcagct cttcaatacg cttgatgtag
     4141 ttcagcgccg cctgcggcag accgctacga tctttcacgc cgaaggtgga ttcagaccag
     4201 cccggcatgg tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg
     4261 gtcgtcactt cacgaccatc cggcatacgg taagccacac agagtttcac ctctttcagg
     4321 ccatccagaa cgtccagttt agtcaggcag aagccagaca gggagttcag ctgtaccgca
     4381 cgacgaacgg caacggtgtc cagccagccg gtacgacgac gacgccccgt agttgcgccg
     4441 aattcgttac cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac
     4501 ggacctgcac ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa
     4561 cgcgggccca ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg
     4621 tacggataag taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg
     4681 aaatcgccac gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc
     4741 aggatgtcgg caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct
     4801 ttgtagtagt taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg
     4861 aaggtttctt tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca
     4921 taagcaggcc cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca
     4981 cgcgcgttat ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc
     5041 agacgctcac gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg
     5101 gccggagaca gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct
     5161 gatggaataa gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg
     5221 tgaccgccct ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta
     5281 cctttacctt cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa
     5341 aatcaccgtt tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt
     5401 gcactcaaaa atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat
     5461 gtagtagacc acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc
     5521 gggcaaattg gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc
     5581 taaaccttcc agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta
     5641 tccttataga aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt
     5701 accgcagtcg ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc
     5761 gctatccggg ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg
     5821 caggctacgg atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt
     5881 ggctgcttcg gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag
     5941 cgttctggtc acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg
     6001 tgaacggtga cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc
     6061 agacacttcg gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag
     6121 cgccgccata ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg
     6181 ctctgccgct tcggcaatgg cgttatctgc tgccggggta gtaacttcat cttctgtacc
     6241 cgcagaaccg gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcagt
     6301 gacgatatct ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg
     6361 tttcaacagc acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct
     6421 gaaatcgctg atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt
     6481 cacaaagcgg tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt
     6541 ttcaatgaac ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc
     6601 gtcacgcagt accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa
     6661 gacagacatg taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat
     6721 cgttattccc cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata
     6781 atatcgccct gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct
     6841 ggcggcagac gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttacca
     6901 cctttcagca tctggtctaa cggcagaacc atcaggttgc cacctttgtc gttaaccagc
     6961 actttgcggg tgttgcccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta
     7021 atttccggcg cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga
     7081 gcttccagga tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga
     7141 ccgttcgcac gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt
     7201 tcgttttcac gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga
     7261 gcagcctgga agttgacgtc cagcagcgtg atacccatgt catacggacg aatcgtctct
     7321 tccagttcgc gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc
     7381 atggtgtatt taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca
     7441 tccgggctgg tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc
     7501 atctcaacgc gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca
     7561 cgcacggctt ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga
     7621 cccggctcaa ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct
     7681 ttaatggtat agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta
     7741 acgacacgac cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg
     7801 gtgcctttac cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct
     7861 aaatcaggtg gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca
     7921 ggtttgctgc ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc
     7981 atgtttgtgc tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata
     8041 tgactccacg cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg
     8101 tttacagagg cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc
     8161 ctcctccatc cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgccc
     8221 ttcctgcggt ggcagacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa
     8281 agcctgaaaa agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg
     8341 tttgttctct tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt
     8401 catcaccagc agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc
     8461 ttcgatgttt tcttgtacac gcacatccgc cgcatcaatg acgtgcagca gtaatgtggc
     8521 ttgccgcgtc tcttgtaacg tggctttaaa tgccgccacc agatcgtgcg gcaggtggcg
     8581 aataaaccct acggtatctg caagtacggt ttcaccgaca tctgcaacgt caatacgccg
     8641 caacgtcggg tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat
     8701 gcgattgaaa agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac
     8761 gtcggctttg atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttccaggcg
     8821 tgactgtatc tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc
     8881 acccggacca cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac
     8941 caggcgcgta gccagatggc gcagctgcgc cagctcaacc tgcaacttac cctcatgggt
     9001 acgcgcacgt tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc
     9061 gcacaaacgc tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac
     9121 caaagcaccc gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa
     9181 atactttggg tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc
     9241 ggaagagacc agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata
     9301 gatgtgtacc agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct
     9361 ctaaatagat cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga
     9421 aaccttattc ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat
     9481 gatggtagtt actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag
     9541 acgggacaac agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca
     9601 ggatcacgaa ttgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat
     9661 aaatagaaac tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt
     9721 gccccttagc cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc
     9781 tgaaaaaatt gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca
     9841 acctgtaata cttcgtcgcg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct
     9901 tcccaaccac gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct
     9961 cgataaacca tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa
    10021 cccacgcaac gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg
    10081 acttctgctt caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg
    10141 agcagttcac ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct
    10201 cctgacgttt gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc
    10261 cgggaaagcc tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc
    10321 tgaagttgac gatgcaatga ctcccaacct tgctctaccg cctgttgctc aattctggcc
    10381 cgcacttccg ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac
    10441 aacatcgtac cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc
    10501 gccagcgcat cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc
    10561 agcaatcggt gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc
    10621 atccctttgt aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc
    10681 agctcaatgg ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta
    10741 ggcaggctcg ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc
    10801 aacagattgt aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac
    10861 gtccgccagc agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt
    10921 tcgtgcaatc cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc
    10981 tatcagttca ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct
    11041 gatggtcaca tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg
    11101 cgctttttct aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaatcagcaa
    11161 cggctgggcg caaactggcg cttcacccgg cgtcagttgc gcctgacgca gccaacgttc
    11221 tgccactggc aaggctaaaa gtgaaatgtt gccatcgcgc tccagcaacg cacagtcgga
    11281 atggacgata gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg
    11341 cggttccggc gcttttggtt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac
    11401 ttcaccttgc tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact
    11461 gcctgaggcg ggcgcaggag agtagcgcgg agctaccggc tcacgagctg ccggttctgc
    11521 aaagtgattg cgccccgccg ccacgcggtt ttccggaatg ggacgcggtg caggttgggg
    11581 ttcatcgtcc agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg
    11641 atagataaaa tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg
    11701 tacgttgacg tccacctgat gcgggtcgat ctccagatac aacacaaacg ccggttgttg
    11761 atcggccccc agtttgtctt cgcaggcctg gcggatcgca tgattgatca ggcgatcgcg
    11821 catcatgcga ccattcacgt agcaatactg aatttccgcc agtgcgggcg tagtgtgatt
    11881 tggatcggcc acccagccgc gcagcgtgag atcgccgtgt tgccattcaa tcgccagcgc
    11941 ttgttcgaga aaagcggtgc cacaaatcgc gcctaagcgc cgttcttttt gcccgccttc
    12001 cggcactgcg cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc
    12061 gaaacgcgcc agcgcaatgc ggcggatgat ttcatcaata tggctaaatt cggttttctc
    12121 ggtgcgcagg aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt
    12181 cgtccccaca ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata
    12241 ggcctgccag gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga
    12301 actgatactc gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc
    12361 cagagaggcg attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt
    12421 gataccgcag ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat
    12481 atcgatacgc gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc
    12541 aggtcgctcg accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg
    12601 aattggcatc aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc
    12661 agtttgtgcc gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg
    12721 caggcctttg taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg
    12781 ttcttcgctg ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga
    12841 acgcagaacg ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg
    12901 caactgactg atcatactgg tcgctacatc ataccctacc cgttgggaat gaccgaactg
    12961 taaatccagc accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcacc
    13021 cgccccaccc agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct
    13081 gttggcgcga cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc
    13141 ggcatcagcg tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcactgcg
    13201 ccccatcacc gagataaagt aatccccgtc acgggttaaa acgcccttaa acatcggatc
    13261 gtcattgagc aaagtacgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt
    13321 accaccgggg ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat
    13381 aattttatcg ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct
    13441 gataacaccc gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac
    13501 gcgcggtgcg acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gcggaggagg
    13561 aggtggcggc gcatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg
    13621 cttcaccgct tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc
    13681 atctttaggc gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg
    13741 cagtccctga atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg
    13801 gctaaacgca taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc
    13861 gttagaaacc tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcgt
    13921 cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc
    13981 taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc
    14041 ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg
    14101 ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa
    14161 ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgataaacca ttaaattgtc
    14221 gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag
    14281 agcttgtaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata
    14341 gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg
    14401 ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa
    14461 caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg
    14521 ccgccagtac gtcagctgca gcaccgtgcg cgacacagcc cgcacaggcg gcatcatacg
    14581 gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc
    14641 cgctcgccat gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga
    14701 ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg
    14761 cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct
    14821 cgccaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt
    14881 tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagtgctt
    14941 ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca
    15001 ggctttcggt aagagagtcc atcgtcagtt catgcaccat caattccggt cgtgcagtca
    15061 gcagcggcgc aatgttttca ctgcgggtca gtactcggac taaaccagca ccagcacgta
    15121 gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatta
    15181 ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggttttagc cagtgagaaa
    15241 gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccaa ctatccagcc
    15301 ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg agcaagcctg
    15361 gtttcagcgc aataaaagtg atggtgtgat cggcgttgat cactgcgcct ggcgtagcgc
    15421 cggtttcagc cagcaggccg gaagggatat caaccgccgc aatcggcgca ggatgggaat
    15481 tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtaccga
    15541 gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct
    15601 cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt
    15661 cgctctcctg ggccaacaac gtgacctcaa tgccgaccgc tgtggccagt cgcgcgacca
    15721 cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag
    15781 gatacgccga acgacacacc tggaatgcgg cttcgccagc gcgaagcatc agctcataga
    15841 gtgtaagccc cagcgcatct gccgcctcac gttctccgcg gcggatatcg tcggcgtgcc
    15901 agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc
    15961 ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc
    16021 tgcaggtagg tattaccgat accgatctca gcgagtccga gcccaaactg caagcatggc
    16081 tggacaaaca ataccacggc gaaatggact ggatggcacg tcacggtatg ctgcgcgctc
    16141 gcccccatga gttattaccc ggtacgctgc gcgtgatcag cgtgcggatg aattaccttc
    16201 ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa actcggctat gttagccgtt
    16261 atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg actcaaaaag ctgggcgaga
    16321 tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc
    16381 tcgagcgccc gttagctgca aaagctgggc tcggctggac aggtaagcac tcacttatcc
    16441 tcaatcgcga ggccggttct ttcttctttt taggcgaatt gctggtcgat attccgctgc
    16501 ccgtggatca accagtcgag gaaggatgcg gtaaatgcat agcctgtatg acgatttgcc
    16561 cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca
    16621 ccatcgaact ggaaggggcg atcccggaag agttgcgacc gttaatggga aaccgtattt
    16681 acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa ctcactacag
    16741 aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag ttattcgcct
    16801 ggagcgaaga gaagttttta aaagtcacgg aaggatcggc gattcgccgt ataggtcacc
    16861 tgcgctggct gcgtaatatc gccgtggcat taggcaatgc cccctgggat gaaacgattc
    16921 tggcggcgct ggagagtcgc aaaggtgagc acccacttct tgatgagcac atagcgtggg
    16981 cgatggcgca gcaaatcgag aggcgaaatg cgtgcatagt cgaagtgcaa ttgccgaaaa
    17041 aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca
    17101 gcttgtgaat aaaaataaaa acgcattgca attcaagacg aataaaatcg tcaagtgatc
    17161 gactcgacaa aacgaaatct taatttttaa cttatctatc aataagttaa aaaaatacta
    17221 ttcaccgtgc gaagtttctt tgaaattaag actacagcct gaggctgtgg ataactctgt
    17281 ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc gctgtggata
    17341 ttttattgag agaagaattt gg
//
LOCUS       DAZUFB010000101        17244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZUFB010000101 DAZUFB010000000
VERSION     DAZUFB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.101"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..4052
                     /locus_tag="ACHY3M_003800"
                     /pseudo
     CDS             1..4052
                     /locus_tag="ACHY3M_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            4275..4541
                     /gene="hipB"
                     /locus_tag="ACHY3M_003801"
     CDS             4275..4541
                     /gene="hipB"
                     /locus_tag="ACHY3M_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFS4318630.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4541..5863
                     /gene="hipA"
                     /locus_tag="ACHY3M_003802"
     CDS             4541..5863
                     /gene="hipA"
                     /locus_tag="ACHY3M_003802"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFS4318631.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6175..6383
                     /locus_tag="ACHY3M_003803"
                     /pseudo
     CDS             6175..6383
                     /locus_tag="ACHY3M_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6815..7378
                     /gene="fimA"
                     /locus_tag="ACHY3M_003804"
     CDS             6815..7378
                     /gene="fimA"
                     /locus_tag="ACHY3M_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4318632.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILT
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            7739..8449
                     /locus_tag="ACHY3M_003805"
     CDS             7739..8449
                     /locus_tag="ACHY3M_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4318633.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            8491..11142
                     /locus_tag="ACHY3M_003806"
     CDS             8491..11142
                     /locus_tag="ACHY3M_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4318634.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTIFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            11156..11686
                     /gene="ydeS"
                     /locus_tag="ACHY3M_003807"
     CDS             11156..11686
                     /gene="ydeS"
                     /locus_tag="ACHY3M_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318635.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            11699..12202
                     /gene="ydeR"
                     /locus_tag="ACHY3M_003808"
     CDS             11699..12202
                     /gene="ydeR"
                     /locus_tag="ACHY3M_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318636.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12261..13175
                     /gene="ydeQ"
                     /locus_tag="ACHY3M_003809"
     CDS             12261..13175
                     /gene="ydeQ"
                     /locus_tag="ACHY3M_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318637.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            13509..15788
                     /gene="ydeP"
                     /locus_tag="ACHY3M_003810"
     CDS             13509..15788
                     /gene="ydeP"
                     /locus_tag="ACHY3M_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFS4318638.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMSDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            16036..16233
                     /gene="safA"
                     /locus_tag="ACHY3M_003811"
     CDS             16036..16233
                     /gene="safA"
                     /locus_tag="ACHY3M_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFS4318639.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            16308..17069
                     /gene="ydeO"
                     /locus_tag="ACHY3M_003812"
     CDS             16308..17069
                     /gene="ydeO"
                     /locus_tag="ACHY3M_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFS4318640.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYALSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT         4845 a         3734 c         4178 g         4487 t
ORIGIN      
        1 cgtcggaaac ctcaatatta gcgatggcgg taagttcgta tcgcaaaata ttactttttt
       61 gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgcgacat catcgttcga
      121 tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca gtaatggtgc
      181 cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta aggggatagt
      241 taatatttca acggacagtc tctggaattt aaagacatca tctactaacg cacaattgct
      301 acaggtcggt gtattaggca agggtgaact gaatattacc accggaggta tagttaaagc
      361 gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg tggatgggca
      421 gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg gtacgttaac
      481 cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag gtgtttttga
      541 acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg cagatgccgg
      601 gtatataacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt ttgtctttaa
      661 tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacag gtgacgataa
      721 agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga atacttatag
      781 cggtaaaacg ctggtcaatg acggcctcct gaccattgcg tctcatacgg cagatggggt
      841 aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg acattctcgc
      901 atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg atggcttgat
      961 gcgagtgcag ctgtcatcct acgacaagat gtttggcttt acccatgcaa cagggactga
     1021 attcgccggt gttgcccaac tgaaagacag taccttcact ctggaacgcg acaacaccgc
     1081 tgcgcttact cacgcgatgt tgcagtctga cagtgaaaat accacatcgg taaaagtggg
     1141 agagcaatcc attggtggac tggccatgaa tggcggtacg ctcattttcg atacggatat
     1201 tcctgctgcg acgcttgcag agggatatat cagcgtcgat acgctggttg tcggcgcggg
     1261 tgactacacc tggaaaggcc gtaactatca ggtaaacggg acgggcgacg tgcttatcga
     1321 cgtgcctaaa ccgtggaatg atcccatggc gaataaccct ctgacgacgc tcaatttgct
     1381 ggaacacgat gatagccatg tcggcgttca actggtgaag gcgcaaacgg ttattgggtc
     1441 gggtggctca ttaacgttac gtgatttaca gggcgacgag gtggaagcgg acaaaacgtt
     1501 acacattgcg caaaacggaa cggtggtcgc cgagggtgat tatggattcc gcctcacgac
     1561 cgcaccaggt gatggtttgt acgttaacta tgggctgaaa gcgctgaaca tccatggtgg
     1621 gcaaaagctg acgttagccg aacatggcgg agcctatggc gcaacggccg atatgtcggc
     1681 aaaaatcggt ggtgaagggg atctggcaat caatacggtg cgacaggttt cgctttccaa
     1741 cggtcagaac gactatcagg gggcaaccta cgttcagatg gggacattac gtaccgatgc
     1801 ggatggtgca cttggcaaca cccgggaact gaacatcagc aacgcggcca tcgtcgatct
     1861 taatggatcg acgcagacgg tagagacatt caccgggcag atgggttcga ctgttttgtt
     1921 caaagagggg gcgctgacgg taaataaagg tgggatcagt cagggtgaac tgacaggtgg
     1981 cggaaacctg aatgttacag ggggaacgct ggctatcgag gggcttaatg cacgctacaa
     2041 tgcgttaacc agcattagcc caaatgcgga agtcagcctc gataatacgc aggggttagg
     2101 cagaggaaat attgccaata acggtctgtt aacgctaaaa aacgtgactg gcgaactgcg
     2161 caatagcata agcgggaagg gtatcgtgag cgcaaccgcc aggacagatg tagagctgga
     2221 tggcgataat agccgctttg tggggcaatt caacattgat acaggcagcg cgctcagcgt
     2281 caacgagcag aaaaacctgg gtgatgcttc cgttatcaat aatggcctgc tcaccatctc
     2341 cactgagcgt agctgggcga tgacgcacag tatcagcggt agcggtgatg tgacaaaact
     2401 gggtaccggg atcctgactc ttaacaacga ttccgcggcg tatcagggta cgacggatat
     2461 cgtggggggg aaattgcttt cggttccgac tctgccatta atatggcaag tcaacacatt
     2521 aatatccata acagcggtgt gatgtcggga aatgtcacca ctgcaggtga tgtgaacgtt
     2581 atgcctgggg ggacactgcg tgtcgctaaa accacaatcg gcggcaacct ggagaatggc
     2641 ggcacggttc aaatgaatag cgaaggggga aaccggggaa tgtactgacc gttaacggca
     2701 actataccgg aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc gataattcgc
     2761 ccaccgataa aatgaacgtg aaaggcgata cccaagggaa cactcgcgtt cgggttgata
     2821 acattggcgg cgtcggtgca caaacggtca acggtattga actcattgag gttggcggta
     2881 attctgcagg taacttcgcg ctgaccaccg gaactgtcga agctggggct tacgtctaca
     2941 cgctggctaa agggaagggg aatgacgaga aaaactggta tctgaccagt aaatgggacg
     3001 gcgtaacgcc agcggataca cccgatccca tcaataatcc ccctgttgtg gatccggaaa
     3061 gcccatcagt ttatcgcccg gaggccggaa gctatatcag caacattgcc gcagccaact
     3121 cgctgtttag ccatcgttta cacgaccgtc tgggtgagcc gcagtatata gattcactgc
     3181 attctcaggg gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa
     3241 gggccggtga cggtcagcta attactcagg ctaaccgcta tgtattgcag ctaggcggcg
     3301 atttggcgca gtggagtagc aacgcgcagg atcgctggca tcttggcgtg atggcaggct
     3361 acgccaatca gcacagtaat actcagagta atcgtgtggg ttataaatcg gatgggcgca
     3421 tcagcggtta cagcgctggg ctgtacgcga cctggtatca gaacgatgcg aataagaccg
     3481 gcgcttatct tgacagctgg gcgctgtata actggtttga taacagcgtc agttccgata
     3541 accgttctgc tgacgactat gattctcgcg gtgtgacggc ctctgttgag ggtgggtata
     3601 cctttgaagc gggaacattt agaggcagcg aagggacgct gaatacctgg tacgtccagc
     3661 cacaggcgca aatcacctgg atgggtgtga aagattctga ccataatcgg aaagacggaa
     3721 cacgcattaa aacggaaggc gacggaaacg tgcaaacgcg acttggggtg aaaacctacc
     3781 tgaatagcca tcaccagcgt gacgatggta aacagcgtga gttccagcct tacattgaag
     3841 cgaactggat caacaatagc aaagtctacg ccgtgaagat gaatggtcaa accgtaagcc
     3901 gtgatggtgc gcgtaatctc ggtgaagtac gtaccggggt tgaggcgaaa gtaaataaca
     3961 accttagcct gtgggggaat gtcggtgtgc aactaggtga taaaggctat agcgatactc
     4021 agggcatgct gggagtgaaa tatagctggt aaaccgtata agccgcatgt cgagatggca
     4081 tgcggcttaa tattgccgac ttcaaacggc gcatcagcgc cttttttaaa tcctcctttt
     4141 tatccgcgat cgcggatatc gcagcgttta tcccgtagag cggataagac gtgtttccaa
     4201 attgacttat cctcactaaa ggataaaact gataatatcc tcttatgcgg ataaatttgc
     4261 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
     4321 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
     4381 gcattaagca ggcgacaatt tccaatttcg aaaacaaccc tgacaatacc acgctcacga
     4441 cattttttaa gattttacag tcgcttgaac tctcaatgac actatgcgac acgaaaaatg
     4501 cctcgccaga atcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
     4561 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
     4621 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
     4681 gcagaggggg aatatcacct cagatgccgt atttaacttc ttcgataacc tgttacccga
     4741 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gctaaatcca gacaaccgtt
     4801 tgatttattg tcagaaatag ggcgagacag cgttggtgcc gtaacgttat tacccgaaaa
     4861 tgaaaccata acgcgcccga taatggcatg ggaaaagctt actgaagcca ggcttgaaga
     4921 agtattaacg gcttataaag cagatatccc tctgggcatg attagagaag aaaatgactt
     4981 tcgcatctcg gttgctggcg cgcaggagaa gacagcgctg cttagaatag gcaatgactg
     5041 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     5101 cagacagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     5161 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaaa
     5221 tgtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     5281 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat ctgtgaaata
     5341 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     5401 ggcgcttcgc gatcgttatg attttatgaa attccaggtc ttccagtggt tgattggcgc
     5461 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     5521 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaatacacat
     5581 cagcgatctc aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga
     5641 taaaatttat ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc cggaagtgca
     5701 gatgcatgaa atcctaagtg actttgccag aatgattccg gcagcactgg ataacgtgaa
     5761 gacttcatta ccgacagatt ttccggagaa cgtggtgacg gcagttgaaa ccaatgtgtt
     5821 gaggttgcat ggacggttaa gccgagaata cggtagtaag tgagatatgg ggtttggtcg
     5881 ttgttaatga gcacgacaat catgaccgcc aacgactgga ttcttccttg gtttatactg
     5941 gtgcgcataa tgtatattat gttaaatcat gtctgagtga tgcgaagtgc ttttctggta
     6001 gtcgttattc gttcagggtt caagaactta agtctctcct ctttcataat tggcctgtgc
     6061 atggagaaat agcctggtta ttccctgccc catgctaaga gcgcagtgtc tgtcgcatag
     6121 acactgcata cgaaaacacc agagggtgct gttacttatt taaggacgga tcaagcggga
     6181 atgagtccac tccggtatca gaaatggcta cgtctcaatg aagtcaggcg accgatgctg
     6241 aatgaacatt acgatgtcac cacagcagcc tatgctgtcg gttacgaaag ctatcccatt
     6301 tcagtcggga atattcgcgg atgtttggag agtcacccaa gagagatatt accaggttaa
     6361 gaaagtctgt cggtcagctt taataaaccc tgcacttatc tgtttagagc aattcggtgt
     6421 tagtttcagc aagcaaacat taaccatagc taatgattta tagccatatt aaccattggt
     6481 agcccttgac ctttcctgac acttcccttg ttcgcaatga tattctgttt agtcacggtt
     6541 tataaataac atgcataatg aaatcgtcat gttatttagc gacataaaat gtttttgcaa
     6601 atactctctt caacaagtgc acaataaact tgttgtgatt aggtgtcctt gtgatttaaa
     6661 gatattatcg ttcttcgtga aatgtaataa ccctggaaac tatattctca gggcaacaac
     6721 caatcaagaa tgattattgt ttttaagcaa taacagaatg acttaattgt taaccctctt
     6781 tattcagcta cttaaatata aattttggag aattatgaaa ctcaaacatg ttggtatgat
     6841 tgtcgtttct gtgttggcga tgtcgtctgc tgcggtaagc gcagccgagg gtgatgaatc
     6901 agtaacgacc actgttaatg gcggtgttat tcattttaaa ggtgaagtgg taaatgccgc
     6961 ttgtgcaatt gattcagaat caatgaacca aacggttgag ctgggtcagg ttcgttcttc
     7021 tcgcctggct aaagcgggtg acctcagctc cgccgttggc ttcaatatca agctgaatga
     7081 ttgtgatacc aatgtttcca gtaatgcagc tgttgcattc ctgggtacta ctgtcaccag
     7141 taatgacgat acgttagcgc tgcagagttc agcggcaggc tctgcccaaa atgtcggtat
     7201 tcaaattttg gaccgtacgg gtgaggtatt aatacttgat ggggccactt ttagtgctaa
     7261 aaccgacttg attgatggca cgaatatact gacattccag gctcgttata ttgctctcgg
     7321 gcagtccgta gctggtactg caaacgctga tgcgaccttc aaagttcaat atctataatt
     7381 caatcttaag gtggctatac gtcatctgaa attgaaacag ggtgtgtgtg gcatggctac
     7441 gcacacccga gcaatttacc aatattcatc tcatttttaa tgagaaatgg tttgtatttg
     7501 gagcgctatg aaaaatcaca taatatctaa actatcaata aacaacagta gttaaactta
     7561 ctgttattta aaatatttac tttgtagcaa ggttttccta ttaaaagaga accaacattt
     7621 attacaaacg cattaagtct ggaaacccgg cgactcgcca atgtattagc aacattaatc
     7681 gattgtctga tgtaacggtt ttcccgctta tcctcttaac acatggagaa tgcatcagat
     7741 gcaaactacg cgtaccccct actctatttc ctttatggcg acagtgttac ttttattgct
     7801 ctttgcttgc catagcaccg ttgccaacgc agctgtcgca ctgggtgcga cgcgggtaat
     7861 ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc ctgccagtgt
     7921 ctatctcatt cagtcatgga tagaaaatgc aggtgaccaa aaagacaccc agtttgtcat
     7981 taccccaccg cttttttcca tgcaaggtaa aaaagagaat acgctgcgga taatcaacgc
     8041 tactaatcat cagttaccag gcgaccgtga aagcctgttc tgggtgaatg ttaaagctat
     8101 tcctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa ttattagccg
     8161 cataaaaatg ttttatcgcc ccacgaacct ggcaatggca cctgaagaag cgcctgcaat
     8221 gcttcggttt cgtcgcagcg gtagcaaact cacgcttatt aatccaacgc cctatttcat
     8281 taccgtaaca aatatgaaag caggaaatag caatctccca aacaccatgg tgccgccaaa
     8341 aggtgaagta tcggtggata tcacgcatgc tgcaaccggg gatatcagtt ttcagacgat
     8401 caatgattac ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag ccagtgacct
     8461 aattaataat aaactcaatc ggaattaata atgacagcct ttcgtgccgc atttaaggca
     8521 tatcgtatgc atcaggtgtt aataatgcct cgctttgcac ggcttaccat tgcccttggc
     8581 ttagcaactg cggtatttcc cgttgacgca gagtattatt ttaatccacg gtttttaagc
     8641 aatgatcttg ctgaatctgt cgatttatcc gcctttacta agggacgcga ggcaccacct
     8701 ggtacatatc gggtcgacat ttatttgaac gacgaattta tgactagtcg ggatattact
     8761 tttattgcag atgataataa cgcagaactc atcccatgcc tgagtacaga tcttcttgtc
     8821 agtcttggta taaaaaaaat agcgttatta gacaataaag aacattcggc agaaaaacat
     8881 gtgccggaca acagcgcatg cacaccactt caggatcgtt tggccgacgc atcaactgag
     8941 tttgatgttg gtcagcaaca tttgtcactc tccgttccgc aaatttatgt tggcagaatg
     9001 gctcgcggct atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg gcttctaaac
     9061 tatagtttta atggtaattc tattaataat cgtagtaacc ataatgcagg aaaatccaac
     9121 tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact gcgcgataac
     9181 tcaacgtgga gttataacag tgggagtagc aattcatctg acagcaataa atggcagcat
     9241 atcaatacgt cggctgaacg tgacattatt cccttacgct cacggttaac ggtaggtgat
     9301 agttataccg atggcgatat ttttgatagt gtgaactttc gtggcctcaa aataaattca
     9361 acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca tggtattgcc
     9421 cgtggaaccg cacaagtgag tgtaaaacag aacggatacg atgtttatca gactactgtt
     9481 ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg tgatctgcaa
     9541 gtaaccataa aagaggcaga cggcagtatt cagacattat atgttcctta ttcgtctgtt
     9601 ccggttctcc aacgtgctgg atatacgcgt tatgcgcttg ccatggggga atatcgtagt
     9661 ggaaataacc tgcaaagctc tcccaagttc atacaaggta gcttgatgca tggtctggaa
     9721 ggaaactgga caccttatgg cggaatgcaa attgcagaag attatcaggc cttcaacctt
     9781 ggtattggga aagatttagg actttttggt gccttttctt tcgatatcac gcaggccaat
     9841 acgacacttg cagatggcac ccgtcacagt gggcaatcgg ttaaatccgt ctacagcaaa
     9901 tccttctacc agacaggaac caatatccag gtcgcaggat atcgctattc tacgcaaggt
     9961 ttttataact tatccgacag tgcctacagt cgaatgagtg gttacaccgt caagcctcct
    10021 accggagaca ccaatgagca gacacaattt attgattatt ttaatctgtt ctacagtaag
    10081 cgtggtcagg aacaaataag catctctcag cagcttggaa attacggtac gatatttttc
    10141 agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat atcatttgga
    10201 ttaaatgtgc cgtttggtga tattacgact tcgctgaatt acagctattc caataatata
    10261 tggcaaaacg atcgggatca tttactcgct tttacgctta atgttccctt cagtcattgg
    10321 atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag tatgtcaaac
    10381 gatttgaaag gcggcatgac caatctatcg ggggtttatg gcactctgct gccggataat
    10441 aacctgaatt atagcgttca ggtcggtaac acccacggag gtaatacatc gtctggcacc
    10501 agtggttaca gttctcttaa ttatcgtgga gcttatggta atactaatgt cggttacagt
    10561 cggagtggtg acagcagcca gatttattac ggaatgagtg gtgggattat tgctcatgct
    10621 gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa ggctcctggt
    10681 gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg tggctatgcc
    10741 atattaccat ttgcgacaga atatagagaa aaccgtgttg ctcttaacgc gaattccctt
    10801 gcagataatg ttgaactgga tgaaaccgtg atcactgtca tcccaactca cggtgctatt
    10861 gccagagcaa catttaatgc acaaatcggc gggaaagtat taatgacgtt gaagtacggt
    10921 aataagagtg ttccattcgg tgcaattgtc acacacggag agaataaaaa tggcagcatt
    10981 gtcgcagaaa acggtcaggt ttatctgact ggacttccac agtcagggaa attacaggtt
    11041 tcatggggca aagataaaaa ctcaaactgt attgtcgagt acaagcttcc tgaagtttct
    11101 cctggtacct tactgaacca gcagacagca atctgtcgct aaggaacaat agattatgaa
    11161 atacaataac attattttcc tcggtttatg tctggggtta accacctatt ctgctttatc
    11221 cgcagatagc gttattaaaa ttagcgggcg cgtcctcgat tatggctgca cagtctcatc
    11281 ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat ttccaacgac
    11341 cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca gcaaagggac
    11401 aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata ctttgttgaa
    11461 actggatgaa ggaagcaata cggcctccgg tttgggtata gaaatattgg acgcaaatat
    11521 gcgtccggtg aaactgaatg accttcatgc cgggatgcag tggatcccac tggtaccaga
    11581 acagaacaat attttgcctt actccgctcg tctgaagtca actcagaagt ccgtcaatcc
    11641 gggactggtg agggcttcgg caacctttac ccttgaattt caataatcag agtcagatat
    11701 gaaaagactt cacaagaggt tcctgttagc tacgttttgc gcgttattca cagcaactct
    11761 ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac cctgcactat
    11821 tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acgcgcaatc tgcaacaacc
    11881 tggttctgca tctggctggc acaatattac tttgtcatta accgattgtc cggttgaaac
    11941 aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt acaaaaatga
    12001 aggtactgcc gaaaatattc agatagagct gagggatgac caggatgctg cgttaaaaaa
    12061 tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt ttccacttaa
    12121 ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg cgctaatcaa
    12181 tgtgatctac acctggcaat aacgtcgatt cgattttctt agccttcttt ttaaatcaat
    12241 ttttaacctt aggatatatc atgggtaaaa caatcagtat aaaggtcctg ttcggtattt
    12301 atcttctatt aatggcaggc aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa
    12361 gtattggagc aggtacaacc tctgtttatg ttaatctcga ccctgtaata cagccgggcc
    12421 agaatctggt tgtagacttg tctcagcata taagttgctg gaatgattac ggcggctggt
    12481 acgacactga tcatataaac ctggtacaag gctcagcttt cgcaggctcc ctacagtcat
    12541 ataaaggatc gctgtactgg aataatgtga cctacccctt cccgttgacg acaaatacca
    12601 acgtattgga tattggtgat aagacaccga tgccattacc gttaaaactc tatattaccc
    12661 cagttggggc ggccggtgga gttgtcataa aagcggggga agtcattgcc cgtatacata
    12721 tgtataaaat tgcaacatta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt
    12781 caaataacaa tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaccg
    12841 tggatctccc ggactttcct ggaagcgcag aaatacccct tggcgtatat tgctcaagcg
    12901 aacaaaagct ctcattttat ctttcgggtg ccaccactga cagtagccga caagtgtttg
    12961 ctaacacagc acctgatgca acaaaagcat cgggagtggg tgtcactcta atgcgtaatg
    13021 gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac
    13081 ctctgggttt aagcgcaacc tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc
    13141 agtcagtcat tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc
    13201 gatccattca cgaaaattgc gcggataatt attttgtgaa ggctattagc ctacacctgt
    13261 aagaaaatcc gcgcacaatt gaagccagag tcttattttt tatattgaaa aatatgttaa
    13321 tttaaacact atgagagatg atgcttaact tatcgtattt aatctattgt ttaacgataa
    13381 tagattaaaa atagaaagag agtagtcccc tactcttata tatccatgtt ggcgataatc
    13441 cctttgtatg tactgtgcat catcgctatt acaaatccta ataattcatt tccacacagg
    13501 ataagtagat gaagaaaaaa attgaatcct accagggtgc tgcaggtggt tggggggctg
    13561 ttaaatccgt agcgaatgca gtacgtaagc agatggatat acgccaggat gttattgcca
    13621 tgtctgacat gaataagcca gagggctttg actgtccggg ttgtgcatgg ccagatccta
    13681 agcacagtgc gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca
    13741 cggataagca ggtaaatgcc tctttctttg ctgagaatac ggttcaatca ttactttcct
    13801 ggggagatca cgagcttgag gctgcgggac gtctcactca gcctttgaaa tatgatgccg
    13861 tcagcgactg ttacaagcca ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc
    13921 ttcaaagcta tagtgatccc aatcaggttg aattctatac ttcgggccgc acttccaatg
    13981 aagctgcctt tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact
    14041 gctccaacat gtgccatgaa ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta
    14101 aagggaccgt gttgctggaa gactttgaga agtgcgattt agtcatttgc attgggcata
    14161 accctggtac aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag
    14221 cgaaaatgat cgccatcaat cctctacagg aacgtggcct ggagcgattt accgcaccgc
    14281 aaaacccgtt tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactacaacg
    14341 tgcgcattgg tggcgatatg gcgttgctca aggggatgat gcgcctgtta attgagcgcg
    14401 atgatgctgc aagtgccgca ggtcggccct cattgcttga tgacgaattt attcaaacgc
    14461 ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg
    14521 aacgtatttc tggactaagt cagacacaaa tcgccgaact ggctgacgca tatgccgctg
    14581 ccgaacgcac cattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga
    14641 acgtacagca actggtcaat ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg
    14701 gtatatgccc actacgtggt cactctaatg tacagggcga ccgaaccgtc ggtatcaccg
    14761 agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc accccacctc
    14821 atgcacctgg acatgctgca attgccagca tgcaagcaat atgtacgggg caggctcgag
    14881 cattgatctg catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg
    14941 taccgttaac gcaattagat ttggcggtac acgtagccac taagcttaac cgctctcatc
    15001 tgctgaccgc acggcatagc tatatcctgc cggttctggg gcgtagcgag attgatatgc
    15061 aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt catgcctcgc
    15121 gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa
    15181 tcgcccaggc aacactaccc cagagcgtgg tagcctggga gtatctggtg gaagattatg
    15241 atcgcattcg caatgacatt gaagctgtgc tgccagagtt cgccgactat aaccagcgca
    15301 tccgtcatcc cggtggtttt cacctgataa atgcagctgc tgaaaggcgc tggatgacgc
    15361 cgtcaggtaa ggctaatttc attaccagca aagggctgtt agaagatccc tcttcagcgt
    15421 ttaacagtaa gctggtcatg gcgacagtac gcagccacga tcagtacaac acgacgattt
    15481 atggtatgga tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg
    15541 ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcgctta
    15601 cgccagacgg taagcgcagc tcacgccgca tggatagatt aaaagtggtc atttacccta
    15661 tggctgaccg ctcactggtg acctattttc cagaatcgaa tcacatgcta actcttgata
    15721 accacgatcc attaagtggc attcctggtt ataaaagtat tccggttgaa ttagaaccat
    15781 caaattaatt cgcctcctca tttcttctgc tgtcatccgc acagcagaag aatttctcat
    15841 tgactattat cgcgcaattt gctcacattg attaaattaa accacacact ataagatata
    15901 aacctctgcc tacagctgta agaaactccg ctcagtactg aagtgccagt cctatttcct
    15961 cttttctcca gcctgttata ttaagcatac tgattaacga tttttaacgt tatccgctaa
    16021 ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac
    16081 tataaagaaa tcatgtctgt aattgtggtt gtcttattac tgacccttac gttgatagcc
    16141 attttttcgg caattgatca gctgggtatt tcagaaatgg gtcgcattgc aagagatctt
    16201 acacatttca ttatcaatag tttgcaagac tgaaaataaa cagcaaatta taaatatgaa
    16261 atgttaaaaa agtatcgata aaaactttat tgttttaagg agataaaatg tcgctcgttt
    16321 gttctgttat atttattcat catgccttca acgctaacat tttagataaa gattacgccc
    16381 tctctgacgg cgagatcctg atggtagata acgctgttcg tacgcatttt gaaccttatg
    16441 agcggcattt taaagagatc ggatttactg aaaataccat taaaaaatat ctacaatgca
    16501 ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac
    16561 cgactggatt gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata
    16621 atttttcaga acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca
    16681 ttacactatt aactaacggt gtgctatccg tttccggaaa agtgagaaat attgtcaaca
    16741 tgaagctggc gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa
    16801 gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg
    16861 ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg
    16921 aacaatgtgg ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca
    16981 acagtccgaa gagagtttct aaggagtacc gttgtcaaag tcacacgggt atgaatacgg
    17041 gcaacacgat gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg
    17101 ctacgtaaga attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat
    17161 ttattgaaat aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat
    17221 gattatttat gatctcaatt cggc
//
LOCUS       DAZUFB010000102        17222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZUFB010000102 DAZUFB010000000
VERSION     DAZUFB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.102"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(239..987)
                     /locus_tag="ACHY3M_003813"
                     /pseudo
     CDS             complement(239..987)
                     /locus_tag="ACHY3M_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1066..2019)
                     /gene="lsrR"
                     /locus_tag="ACHY3M_003814"
     CDS             complement(1066..2019)
                     /gene="lsrR"
                     /locus_tag="ACHY3M_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFS4318641.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2268..3805
                     /gene="lsrA"
                     /locus_tag="ACHY3M_003815"
                     /pseudo
     CDS             2268..3805
                     /gene="lsrA"
                     /locus_tag="ACHY3M_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            3799..4827
                     /gene="lsrC"
                     /locus_tag="ACHY3M_003816"
     CDS             3799..4827
                     /gene="lsrC"
                     /locus_tag="ACHY3M_003816"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFS4318642.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4827..5819
                     /gene="lsrD"
                     /locus_tag="ACHY3M_003817"
     CDS             4827..5819
                     /gene="lsrD"
                     /locus_tag="ACHY3M_003817"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFS4318643.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGLTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5831..6853
                     /gene="lsrB"
                     /locus_tag="ACHY3M_003818"
                     /pseudo
     CDS             5831..6853
                     /gene="lsrB"
                     /locus_tag="ACHY3M_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            6880..7755
                     /gene="lsrF"
                     /locus_tag="ACHY3M_003819"
     CDS             6880..7755
                     /gene="lsrF"
                     /locus_tag="ACHY3M_003819"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFS4318644.1"
                     /translation="MADLDDIKDGKDFRTDQSQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7779..8069
                     /gene="lsrG"
                     /locus_tag="ACHY3M_003820"
     CDS             7779..8069
                     /gene="lsrG"
                     /locus_tag="ACHY3M_003820"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFS4318645.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8126..8884
                     /gene="tam"
                     /locus_tag="ACHY3M_003821"
     CDS             8126..8884
                     /gene="tam"
                     /locus_tag="ACHY3M_003821"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFS4318646.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAAKITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(8888..9802)
                     /locus_tag="ACHY3M_003822"
     CDS             complement(8888..9802)
                     /locus_tag="ACHY3M_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFS4318647.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10009..11460)
                     /gene="uxaB"
                     /locus_tag="ACHY3M_003823"
     CDS             complement(10009..11460)
                     /gene="uxaB"
                     /locus_tag="ACHY3M_003823"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4318648.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPCDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11687..13105)
                     /locus_tag="ACHY3M_003824"
     CDS             complement(11687..13105)
                     /locus_tag="ACHY3M_003824"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4318649.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13244..13603)
                     /locus_tag="ACHY3M_003825"
     CDS             complement(13244..13603)
                     /locus_tag="ACHY3M_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFS4318650.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13603..14529)
                     /gene="glsB"
                     /locus_tag="ACHY3M_003826"
     CDS             complement(13603..14529)
                     /gene="glsB"
                     /locus_tag="ACHY3M_003826"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFS4318651.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14593..15981)
                     /gene="sad"
                     /locus_tag="ACHY3M_003827"
     CDS             complement(14593..15981)
                     /gene="sad"
                     /locus_tag="ACHY3M_003827"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFS4318652.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAIIVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16082..16963
                     /locus_tag="ACHY3M_003828"
     CDS             16082..16963
                     /locus_tag="ACHY3M_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4318653.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17041..>17222
                     /gene="yneK"
                     /locus_tag="ACHY3M_003829"
     CDS             17041..>17222
                     /gene="yneK"
                     /locus_tag="ACHY3M_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFS4318654.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIF"
BASE COUNT         4333 a         4382 c         4275 g         4232 t
ORIGIN      
        1 cgaagtttat taaacccctt ttgcatccat taatgtcttt aggaatattc gctataaaat
       61 aagggttatg cttaatgctt taagaaaaat agcaattttc ccttgaatat agtactggtg
      121 atggaacgat gaatctgcaa gctggcattt aacaagccag ctctaaaaga agggaaataa
      181 gaaaaactat actcaaaaac taacagccac ggtcatcatg atgtggctgt caatgaaact
      241 ataacccagg cgctttccat aacgacgtcg tcagtccatg atcaaccagc cccagctgat
      301 cctgataaac tgcctgccac ttatcgcgtg aatcctgata aagttcatgc ttttccgggt
      361 ctggtgtgtg cgtccgttcc cagcgaacca ggcgttctcc ggtttctgcc attgatgaaa
      421 aaattccggc accgacgcca gttcgccgct gagcatatag gccagccagt cgctgatcat
      481 ggtgatggtt gatgcctgac ggtaaatatc ggaacgatgg tgcgccagcc aaagtaatct
      541 ggggatggca cttaaagcca gtgtttgtcc ggtcgcgcga taaacttcgt tttcaaaggt
      601 attgttgtgc agttctttaa gttcgctaac ttcgcgcgct gctctggcat ccacattggc
      661 gcaggcccag atcggtgttc cttcattgtt atataaaaca atgccttcac gcatagaaca
      721 tgccgaaacg gcagcgatat actccgggtc tatgccggcg ttgtgcagcg cctggcgcat
      781 acactcacac gccagttgcc agtttttgtt gagatcaaat tccatagaac caggaacgtc
      841 cggtactgcc agatgccgcc actccgcctg tcccactgct atttgattgc ctttcaggtc
      901 gaaaatcaca gcccgaatac ttccggtgcc tgcatccagc gccaacaggt aatactttga
      961 ttctgaaggg gtaaagagtc gagccattag attatcctcg gcttatgcgt tctgtctgtt
     1021 cctctatacg ttctccatca ttcccggtaa taaggtcatg caaatttaac tacgtaaaat
     1081 cgccgctgct gtatcctgat cggtaaccag tgcgttgata taaccgcctt tcattgcagc
     1141 ggcaattgct tcggctttat tttctccccc tgccacgcca acccggacgg gtatggtctt
     1201 cagcgcgctt aaaggtaagc caatcagttc gttatgtatt ttaatatccg tgacaacgtc
     1261 accttttgca tcaaaaaagt agcctaaaat gtcgccaacc gccccttttc gaccaatcat
     1321 taactgttcg ccctggctga tataaccgga gcgaatgatc gtcgcgtcgt cctgttgact
     1381 gacggcccca atgccgacaa tcgccacatc cgctgcttgc gcggctaaca gaacatcttt
     1441 gacgcaattt tcatttttta gcgtacgggc aatgtcagcg gaggatgccc gcaacggagc
     1501 cggaataata ttcacactgc acgccgcgtt aagctgcccg atacccgtca tataagaacc
     1561 gacgccaccg gagagcgtga ccaggcgaat ttgctgtgac gaaataaaac cacttaagcg
     1621 ttgcagcgta ttcatggttg cctcgccaaa accaatcgcc agcatctgtt gtggttgaag
     1681 taaactcatc aacatatgcg ccgcgcctat ccctagtcgc ccgccgacat cagcatccgc
     1741 aagcccaggg atcacccgga catgttgcag cgaaaactga cgacgtaatt gagtttcata
     1801 ttccagacag ccttcaaagc gagaattaat ctgtacgcga ataatgccgg actgatgccc
     1861 tttctccagc aagcgcgaca ctttcaaacg ggtcaggccg agacgatcgc tgatctcgct
     1921 ctgggtcagc ccgtcgtgat agtaaaacca cgcgatccgc gcgacctgtt cttcttcaca
     1981 cattccctgt tctgaaattg ccgaatcgtt gattgtcata attcattctt cactttgaac
     2041 atatttaaat ctttaatgca attgttcagt tcttgcgcat ttatatctgt gatggcagcc
     2101 acagtttgac tctacgagca tgaacaaacg caaccgtgaa aatcaaaata gcataaattg
     2161 tgatctattc gtcggaaata tgtgcaatgt ccacctaagg ttatgaacaa attaaaagca
     2221 gaaatacatt tgttcaaaac tcacctgcaa aactgaacgg gggaaatatg caaacgagtg
     2281 atacccgcgc gttaccgcta ctttgcgccc gctcggttta taaacagtat tcaggggtca
     2341 atgtcctgaa aggcatcgat tttacgttgc atcaggggga ggtccacgcc ctgctcggcg
     2401 gcaatggtgc cggtaaatcg acgttaatga agattattgc cggtattacc cctgctgata
     2461 gcggtacgct ggagattggg ggcaacaact acgccagatt aacgccagtt catgctcatc
     2521 agctgggcat ttatctcgtt ccccaggaac cgctgctttt cccaagcctg tcgataaaag
     2581 aaaacatcct gtttgggatg gcaaaaaaac agctctccat gcaaaaaatg aagaacttgc
     2641 tggcggcgct gggctgccag tttgatctgc atagtctggc aggatcgctg gatgtcgccg
     2701 atcgccaaat ggtggaaatc ctccgcgggc taatgcgcga ctcgcggatt ctgatcctcg
     2761 atgaacctac cgcctcgctt acccctgcgg aaaccgaacg cttgtttact cgcttgcaag
     2821 agctgcttgc tactggcgtg ggtattgttt ttatctcgca taagctgccg gaaattcgcc
     2881 agattgccga tcgaattagc gtgatgcgcg acggaaccat cgccttaagc ggcaaaacca
     2941 gcgaactgtc taccgacgac attattcagg ccatcacgcc agtggtacgg gaaaaatcgc
     3001 tctctgccag ccaaaaatta tggctggagt tacctggtaa ccgcccacaa catgctgccg
     3061 gaacgccggt gctgacactg gaaaatctga ccggcgaagg tttcaggaat gtcagcatga
     3121 cgctcaatgc cggagaaatt ctgggcctgg ctgggctggt gggggccgga cgcacagaac
     3181 tggccgagag acgttctatg gtctgcgtac tttgcgtagc ggacgcatta tgctgaatgg
     3241 taaagagatc aataaattat ccactggaga acgtttactg cgcggtctgg tttatctgcc
     3301 ggaagatcgc cagtcatccg gactgaatct cgatgcttcg ctggcctgga acgtctgcgc
     3361 ccttactcat aaccttcgtg gattctgggc gaaaaccgcg aaagataatg ccaccctgga
     3421 acgttatcgt cgggcgctga atattaaatt taaccaaccg gaacaagctg cacggacatt
     3481 atccggtggc aaccagcaaa aaatcctcat tgccaaatgc ctggaagctt cgccgcaagc
     3541 attgattgtc gatgagccga cgcgcggcgt ggatgtctcg tcccgtaatg atatctacca
     3601 gctgttgcgc agcatctccg cacagaatgt ggctgtgctg cttatctcct ctgatctgga
     3661 agagatcgaa ctgatgacag atcgtgtgta tgtgatgcat cagggcgaaa ttacccactc
     3721 tgcactgacc gggcgcgata ttaatgtcga gactattatg cgcgttgcct tcggcgatag
     3781 tcagcgtcag gaggcatcat gctgaagttt attcagaaca accgtgaaat cacggcattg
     3841 ctggcggtgg tgctgctgtt tgtattaccc ggttttctcg accgtcagta tttaagtgtg
     3901 caaacgctga ccatggttta tagcagcgcg caaatcctga tcctgctggc aatgggcgcg
     3961 acactggtaa tgcttacgcg caatattgat gtttcagtgg gttcgattac cggaatgtgc
     4021 gcggtgctgt tggggatgtt actgaacgca ggatattcac tacctgttgc ctgtgtcgcg
     4081 actttactgc ttggtttgct cgcgggattt ttcaacggtg tcctggtcgc gtggctaaag
     4141 atccctgcca ttgttgccac ccttggcacg ttagggttgt acagaggcat catgttgctg
     4201 tggactggcg gtaaatggat tgaagggtta cccgccgaac tgaaacagct ctccgcaccg
     4261 ctgctgtttg gcgtttccgc gattggttgg ttgacgataa ttctggtggc atttatggcc
     4321 tggctgctgg caaagacggc gtttggacgc agtttttatg ccacgggcga taatttacag
     4381 ggcgctcgtc aactgggtgt tcgtacggaa gccattcgca ttgtggcatt ttcgttgaac
     4441 ggctgcatgg cggcactggc gggaattgtg tttgcttcgc agattggttt tatccccaac
     4501 cagaccggta ccgggctgga gatgaaagca attgcagcct gcgtgctggg cggcattagt
     4561 ttgctcggcg gttccggtgc gatcatcggt gcggtactcg gcgcatggtt cctgacgcag
     4621 atcgatagcg tactggtgct gttgcgcatt ccggcatggt ggaacgattt tatcgcgggc
     4681 ctcgtgctgc tggcggtgct ggtgtttgat ggacgcctgc gttgtgcgct ggaacgtaat
     4741 ctacggcggc aaaaatatgc ccgctttatg acgccaccgc catccgttaa acccgcttcg
     4801 tcaggtaaaa aacgggaggc cgcataatgc gtattcgcta cggttgggaa ctggctcttg
     4861 ccgcactgct cgttattgag attgtcgcat ttggtgcaat taacccgcga atgttagatc
     4921 tcaatatgtt gctgttcagc accagtgact ttatctgcat tggcattgtc gccctaccgc
     4981 tgacgatggt gattgtcagt ggcgggatcg atatttcgtt tggtttgacc atcggcctct
     5041 gcgccattgc attgggcgta ctgtttcaaa gtggtgtgcc gatgccgctg gcgatactcc
     5101 tgaccttact gctcggcgca ttgtgcgggc tgatcaacgc cggattaatt atctatacca
     5161 aagttaaccc gctggtgatt acgcttggca cgctgtatct gtttgccgga agcgctctgc
     5221 tgctttccgg tatggccgga gcgacggggt acgaaggtat tggtggattc ccgatggcgt
     5281 ttacagattt cgctaacctg gatgtgctgg gactccccgt tccgctgatt atcttcctga
     5341 tatgtctcct cgttttctgg ctctggctgc ataaaaccca tgccggacgt aatgtgtttt
     5401 tgattgggca aagcccgcgc gtggcgcttt atagcgcgat tccagttaac cgcaccttat
     5461 gtgcgctcta tgccatgacg gggctggcgt ctgcggtcgc cgctgtgctg ctggtatcgt
     5521 attttggttc agcacgttcc gatctcggtg cgtcgtttct gatgcccgcc atcaccgccg
     5581 tggtgcttgg cggggccaat atttatggtg gttccggttc cattatcggc accgccattg
     5641 cggttttatt agtgggatat ttgcagcaag gtttgcaaat ggcaggagtg ccaaatcagg
     5701 tgtccagcgc cctttccggt gcgctactta tcgtcgttgt cgtaggtcgt tccgttagcc
     5761 tgcatcgcca gcaaattaaa gagtggctgg cgcgtcgggc caataaccca ttgccataaa
     5821 ggatatcttc atgacacttc atcgctttaa gaaaatcgcc ttacttagcg ctcttggcat
     5881 tgccgcaatc tctatgaatg tgtaggccgc agagcgtatt gcatttattc ccaaactggt
     5941 tggcgtggga ttttttacca gcggtggcaa cggcgcacaa caagcgggta aagagctggg
     6001 cgttgatgtg acctacgacg ggccgacaga acccagtgtt tctggtcagg tacagttgat
     6061 taataacttc gtcaatcaag gttataacgc cattatcgtt tctgcggttt cgcctgatgg
     6121 cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg agagtgctga cctgggactc
     6181 tgatactaaa ccggagtgcc gctcttacta cattaatcag ggaacgcccg cccagttggg
     6241 aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa gacaaagcca aagtcgcgtt
     6301 tttctactca agccccaccg ttatggacca aaaccagtgg gtgaaagaag cgaaagcgaa
     6361 aatcgccaaa gagcatcctg gctgggaaat tgtcactacg cagtttggct ataacgatgc
     6421 cactaaatca ttacaaaccg cagaaggaat attaaaagcg tatagcgatc tcgacgccat
     6481 tatcgccccc gacgccaacg ccctgcccgc tgccgcacaa gccgcagaaa acctgaaaaa
     6541 tgacaaagtt gcgattgtcg gattcagtac gccaaacgtg atgcgtccat atgtggaacg
     6601 cggcacggtg aaagaatttg gcctgtggga tgtggttcag caaggcaaaa tttcagtgta
     6661 tgtcgcggat gcattattga aaaaaggatc aatgaaaacg ggcgacaagc tggatatcca
     6721 gggcgtaggt caggtggaag tctcgccaaa cagcgttcag ggctatgact acgaagcgga
     6781 tggtaatggc atcgtactgt taccggagcg cgtgatattc aacaaagaga atatcggcaa
     6841 atacgatttc tgatgtgcat tacttaaccg gagtaagtta tggcagattt agacgatatt
     6901 aaagatggta aagattttcg taccgatcaa tcgcaacaaa atatcccttt tatcctgaaa
     6961 ggttgcggtg cgctggattg gggaatgcag tcacgcttat cgcggatatt taatccgaaa
     7021 acgggtaaaa ccgtgatgct ggcctttgac catggttatt ttcaggggcc gactaccgga
     7081 cttgaacgca ttgatataaa tatcgccccg ctgtttgaac atgccgatgt attaatgtgt
     7141 acgcgcggca ttttgcgcag cgtagttccc ccagcgacca ataagccggt ggtactgcgg
     7201 gcgtcaggtg cgaactctat tctggcggaa ttaagtaatg aagccgtggc gttatcgatg
     7261 gatgatgccg tgcgcctgaa cagttgcgcg gtggcggcgc aggtttatat cggcagcgaa
     7321 tatgaacatc agtcgatcaa aaatattatt cagctggttg atgccggaat gaaagtggga
     7381 atgccgacca tggccgtgac tggcgtgggc aaagatatgg tgcgcgatca gcgttatttc
     7441 tcgctcgcga ctcgaatcgc cgctgaaatg ggggcgcaaa ttatcaaaac ctattatgtc
     7501 gaaaaaggtt ttgaacggat tgttgccgga tgtccggtac ccattgttat tgctggcggt
     7561 aaaaaattac cggagcgcga ggcgctggaa atgtgctggc aggctatcga tcagggcgct
     7621 tctggtgtgg atatggggcg taatattttc cagtctgacc atccggtggc gatgatgaaa
     7681 gccgtacagg cggtggttca ccataacgaa acggctgatc gggcatatga actctatctg
     7741 agtgaaaaac agtaactgcg gatttaagga gaagaattat gcacgtcaca ctggttgaaa
     7801 ttaacgttca tgaagacaag attgacgagt ttatcgaagt ttttcgccag aaccacctgg
     7861 gctctgtaca ggaagaaggc aatttgcgct tcgatgtctt acaggaccca gaagtgaatt
     7921 cgcgctttta tatctacgaa gcctataaag atgaagacgc agtggcattc cataaaacca
     7981 cgccccacta caaaacctgt gtcgcgaaac tggaatcttt aatgaccggg ccgcgtaaaa
     8041 aacgtctgtt caatgggttg atgccgtgag gcgaattcat cagttttatc tacaattggg
     8101 gtaacgcgct gacgggagta aataaatgtc tgactggaac ccctctttat atctgcactt
     8161 tgccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtaccgc tggagaatat
     8221 cgaatacgtt gccgatcttg gctgtggccc aggtaacagc accgcccttc taaaccaacg
     8281 ttggcctgct gccaagataa caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg
     8341 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
     8401 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     8461 gtttcctcat ctggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     8521 taactggctg gagccgaccc atgtgctcat gcgcgaagtt gcctgggaac aaaactaccc
     8581 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     8641 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
     8701 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     8761 cgaacagcag ctttttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
     8821 agagaacgga cagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
     8881 gtaattatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttctgctt
     8941 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     9001 cattgttttc agtaccgaac ggatcttcca gttcttccgc cagacaatcc agcgaaataa
     9061 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
     9121 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
     9181 agggaattgg cgtgtaggca atgcgctcac agcctgccag aaccgctgaa atatcattaa
     9241 gccgatcgtt gaggctaata aactggatat ctgaaagctg cccattgcgg cgctgaaccg
     9301 ccaaccactc ccccattatt aacaagatac ggttagctgg agagttcgaa gccagtacag
     9361 gctgaagatc ttcagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
     9421 atgtcatgcg taaacagtgg gcgaaggcga tttgcaaccg ggcaaactcc cttacacttg
     9481 ccgaatccgg caatgtcgtt tttacctcgc gcagtaacga ccgtgaggca atcatcaact
     9541 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
     9601 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
     9661 gatgcgtgta ccagggcagc atgaaaataa cagcgataga aaagagaaaa ttgagtagta
     9721 agcgcgatga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
     9781 gttgttgtgg acgaacaatc atggttatct tcaggcgtga aaaagtcgcc ctattaaacg
     9841 tgattacgat cacattctca agacgctact tacaaattac ctacaagctt acaacattaa
     9901 caggcgctgc atgtggcatc tgttaatgat aaaggcacat aggatgttgt aactaatata
     9961 gtgaaataag aaacccggtc gaaaccgggt tcagaagtaa cggtgttatt agcacaacgg
    10021 acgtacagct tcgcgcatcc ctttttcaag aatcgcatcc aggtcattag caacctgctc
    10081 gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg tctttttctg ccaacacaat
    10141 cgctaccagt tcctgggtac cgataacacg gtcacgatgc tggctccaga gttgctgata
    10201 acgttccagc cagtgtgcat cgtcctgtac cggatatgtt tctccgttac gctcaccacg
    10261 atagaaggca atcaatgccg ctaatgcgaa agtaaggcgc gccggaagtg tgccttttgc
    10321 cttctgccct gccagcagct gcggcaggat gcgagtgcgg aacttggtca taccgttgag
    10381 cgcgatagac agcagctgat gcttaatgta cgggttacgg aaacgcccgg tgactgcact
    10441 ggcgaaagat tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat
    10501 agctttttca acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag
    10561 ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt
    10621 gcgttctttg tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag
    10681 acgcagttca gtcgctaagg atttcggtcc ctgaatcaca aacaggtaaa agtgttcagc
    10741 ggtgtcgaga aaaccatcgt gataacccag ctcttcttcc agtttagcca cttcatcgca
    10801 cggataaccg gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag
    10861 ccattgaata aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg
    10921 caacgcgtcg ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag
    10981 cgcaccgttg aaatggctaa aacgttcgaa cagcagacgg gtcagttttg ccggataact
    11041 taccgctggc gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt
    11101 attagagaaa acaaagcgca tttccgggtt gtgagccagt ttcaggaatt catcgtattc
    11161 actgtagacg ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc
    11221 ccccttctca ttcaggccac ggataatggt ggtatacaga ccatcctgcg tgctcagtga
    11281 cggtgggaat gaagtttcaa tcggacgaac aacgaccaca ccagaattca gatcggtgtg
    11341 ctcattcagg agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa
    11401 ctgaatgata cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac
    11461 aatgggttcc cttctgatta gtcatacaac ctgcttgaat tggtgcgaca ggttagcaaa
    11521 ctttaatacg ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga
    11581 gaaaagtgtg gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa
    11641 gtggtagaac acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct
    11701 caccgtaacg catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg
    11761 tatcggcttc gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac
    11821 cactaccgac gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt
    11881 gcagttcaac gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg
    11941 acggcactgc aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga
    12001 caatcttctg aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat
    12061 agttatcgtt aatgcttttg aaatagtcga tatcaagcag catgaccgtc agatgttgtg
    12121 tctgtttcag cgatggactt ttcagcgcct cataaagacc ggaccgtgag taaacctgag
    12181 tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat tagcgaattg atcgctgcca
    12241 cgctaaaaga aaccataatt gggcatatcg ccatcgtggc aataccgaga cgtgcggaga
    12301 acatttccgg aatggagaac ggcgaaccga ccgagatatc aatcaccgaa tttgccacca
    12361 gtacgatttc caccgcaccg gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc
    12421 gcactgcaca ccagattaat gcaggcagcg ggaacgccag actccccgct ccgccaatga
    12481 ctaccgaggc aataaccgac acaataagcg cgatggcggg catcatctgt tctgctttaa
    12541 agcgcggcag cactccagga atagccaacg tcagcataca aggcacgatc aacacgcccg
    12601 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
    12661 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
    12721 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
    12781 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
    12841 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
    12901 aatgcagtcg attaagccag acatagcggg caaacacccc cgccattacg ccattcagcg
    12961 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
    13021 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
    13081 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
    13141 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
    13201 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
    13261 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
    13321 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
    13381 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
    13441 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
    13501 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
    13561 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
    13621 taactgtttc gtcaattgtt caaggacggc aatacccgca agcgagttac ctgcatcatc
    13681 cagttccgga ctccagacag cgatggccat ttcatgcgga acaatcgcca caataccgcc
    13741 accaacgcca gatttcgccg gtagccccac ccgccaggca aactcccccg cgttctggta
    13801 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg
    13861 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc tggccagctc
    13921 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacatc
    13981 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
    14041 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
    14101 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
    14161 aaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgct ccatttccag
    14221 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
    14281 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
    14341 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
    14401 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
    14461 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
    14521 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
    14581 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
    14641 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
    14701 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
    14761 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
    14821 attagccagt tccagcgcat gttctgcatc tttcgcaacg atgattgccg caacagggcc
    14881 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
    14941 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
    15001 cagggttttc tccacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
    15061 gagagcattc tcttcatcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
    15121 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
    15181 aacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
    15241 ggcatcgtta agcacaataa atggatccga accgcccagt tccagtacgc attttttcag
    15301 tgccgctcca gcctgtgcgc caatagccgc tcccgcacga acacttccgg tcaccgtgac
    15361 agcagcaatg cgcgaatctt taatcatttg actgacaccg tcgttgtcgg cattcagcca
    15421 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
    15481 gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
    15541 cgcgccacgc atcacctgcc ataacggaaa gttccacggc ataatcgcca gaatcgtccc
    15601 caacggtcga tactcaataa ctgcctgctg attttccacc agcgtaggtt ccgccttcag
    15661 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
    15721 gcgcgcctga ttgattggtt tgcccatttc acgggtgatc atttgcgcca tttcttcgct
    15781 acgggcgcgc agagccttac cgataccacg cagtttttca gcacgataat ctatatttgt
    15841 ctcgcgccag tcgcgaaagc ctgctgccgc cagctgaagt gcgttttcga tatcgttagc
    15901 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
    15961 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
    16021 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
    16081 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
    16141 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
    16201 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
    16261 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
    16321 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
    16381 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
    16441 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
    16501 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
    16561 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
    16621 taacggcagt aacatttatg ccttccgcgc caattgttcg tatcgtcgcc acttcgagag
    16681 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
    16741 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
    16801 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
    16861 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
    16921 actactggat gtgcctgact cggcaaaaca gggttaccaa tgagctattt ttgatagttc
    16981 ttgcagctca aatgccctat atagtgatgt tccgatgact tatgactata tggggcaaat
    17041 atggttacgc cagtaagcat cagtaattac atatctcttc ctgatgattt tcccgtacgt
    17101 aatattgcac ctcaggtaaa agaagtttta aaggacttta ttgatgcgct aagtacaata
    17161 atatgtgatg aagagtggcg cacgtcttta aacatcaact cagccacaaa aaagatattt
    17221 aa
//
LOCUS       DAZUFB010000103        17051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZUFB010000103 DAZUFB010000000
VERSION     DAZUFB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.103"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            171..4262
                     /locus_tag="ACHY3M_003830"
     CDS             171..4262
                     /locus_tag="ACHY3M_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFS4318655.1"
                     /translation="MNKIYSLKYSSLTGGLIAVSELSKKVKGKTGRKLMTASVALSVS
                     LSALPAEASTVSAEIPYQTFRDFAENKGVFTPGATGIEIKDKNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGGLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLITEAAPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNNG
                     VNGQKNNWLLLPEDYIKNIITADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQF
                     VMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGG
                     IIVNYGTTVKWGINGVSGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNND
                     STAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKNTEFSRLQAVDYGAA
                     IINSSTDKSLLTLNLSPLKKDEIAVSVKALDMNAIFQGGHGTAGDLYKTTFYGPTQYY
                     LLKKPKFGSVLMGSLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNM
                     DIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFNLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNKKLSGKDNILLVDFLNKPSGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGT
                     RDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4425..4586)
                     /locus_tag="ACHY3M_003831"
     CDS             complement(4425..4586)
                     /locus_tag="ACHY3M_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318656.1"
                     /translation="MSFDVFHVLSVSFPVTAVPHMTGECNLRYLTENVPFAGTGGQSC
                     MQDLYHSSG"
     gene            4768..5130
                     /locus_tag="ACHY3M_003832"
     CDS             4768..5130
                     /locus_tag="ACHY3M_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318657.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTK"
     gene            5143..5334
                     /locus_tag="ACHY3M_003833"
     CDS             5143..5334
                     /locus_tag="ACHY3M_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318658.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQAFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            5362..5673
                     /locus_tag="ACHY3M_003834"
     CDS             5362..5673
                     /locus_tag="ACHY3M_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFS4318659.1"
                     /translation="MTSPDLIQADLHRMSWSQLAKAAEESTVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDERLFRLSKHHHLYEKKKTKE
                     G"
     gene            complement(5816..5911)
                     /gene="dqlB"
                     /locus_tag="ACHY3M_003835"
     CDS             complement(5816..5911)
                     /gene="dqlB"
                     /locus_tag="ACHY3M_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFS4318660.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELFRELID"
     gene            6410..7699
                     /gene="trbA"
                     /locus_tag="ACHY3M_003836"
     CDS             6410..7699
                     /gene="trbA"
                     /locus_tag="ACHY3M_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFS4318661.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETTETTETRHPADKEKAQ"
     gene            7696..8814
                     /locus_tag="ACHY3M_003837"
     CDS             7696..8814
                     /locus_tag="ACHY3M_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFS4318662.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKKTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            8795..11098
                     /gene="trbC"
                     /locus_tag="ACHY3M_003838"
     CDS             8795..11098
                     /gene="trbC"
                     /locus_tag="ACHY3M_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFS4318663.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LSWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            11577..11807
                     /locus_tag="ACHY3M_003839"
     CDS             11577..11807
                     /locus_tag="ACHY3M_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318664.1"
                     /translation="MKTNMGSTKNGARVPHVLNRFTSMDIDMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(11905..12336)
                     /locus_tag="ACHY3M_003840"
     CDS             complement(11905..12336)
                     /locus_tag="ACHY3M_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFS4318665.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(12404..15115)
                     /locus_tag="ACHY3M_003841"
     CDS             complement(12404..15115)
                     /locus_tag="ACHY3M_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4318666.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLVW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(15127..15459)
                     /gene="mobA"
                     /locus_tag="ACHY3M_003842"
     CDS             complement(15127..15459)
                     /gene="mobA"
                     /locus_tag="ACHY3M_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFS4318667.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            15692..16027
                     /locus_tag="ACHY3M_003843"
     CDS             15692..16027
                     /locus_tag="ACHY3M_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFS4318668.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(16112..16960)
                     /locus_tag="ACHY3M_003844"
     CDS             complement(16112..16960)
                     /locus_tag="ACHY3M_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4318669.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMRDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         4519 a         4422 c         3964 g         4146 t
ORIGIN      
        1 ggcaaaaaca taagattcac ttaacttttt caccttgtag aatcttcttt tttcatggac
       61 atgttatttt gttgatttta aaatatcaaa atgggtgaat gtatcacatc actcataaag
      121 tggatattat cacgaaacta tctattaaac agcagattag cagatacaac atgaataaaa
      181 tttactcact aaaatatagt agccttactg gtgggcttat agctgtatca gagttaagta
      241 aaaaagttaa aggaaaaaca ggcagaaaat taatgactgc ttccgttgct ttatctgtga
      301 gtctttcagc cttacccgca gaggcatcaa cggtcagcgc tgaaatacca tatcagactt
      361 tccgtgattt tgctgaaaat aaaggcgtat ttacacctgg tgcaaccgga attgaaataa
      421 aagacaaaaa tggaaatgca gttggtacgc ttgatgtgcc aatgattgac ttcagcagcg
      481 tgtctcggcg gggctcactg accctgttgt cgcagggata tggtgtatca gcaaaacacg
      541 gaggccttgg tgatgtaaac aatgccagtt ttggctatga taaaaataat tatactgtag
      601 taaagaacaa caaacattcg ggccttgatt tttctctgca tcggttcagc aaactcatca
      661 cagaggctgc acctgctgat ataaatatat ccggacaact tagtgattcg tcacagtata
      721 ctgcttttta cagagcaggc gctggcacac agtatattaa agaacgctca ggtaaacaga
      781 ctcatatccc gggaacattt ttaactggtg gaacagtagg caccccatgg tattcaggaa
      841 ataatttaat atcaagctct ccaggtgaca cctataataa atcacaggga ccattagcca
      901 gttatgggca gatgggtgac agcgggtcgc cattatttgc ttatgattct ctcagtgaaa
      961 aatggtctct ggctggagtc accctgcata ataatggtgt taatggccaa aaaaacaact
     1021 ggttgttatt acctgaagat tatattaaaa atattattac agccgatttc gatcccatta
     1081 tttcatttaa taaaaacagt aaagaacata tgtcatggac atatgatgca gccaaaggcg
     1141 tgggccgtat tcagcaggat gaccaacagt ttgtgatgca tggtaacctg aatggtaatc
     1201 tgaatgcagg taaaaatctt tattttacag gcgaaaatgg cattattgat ttaaaagata
     1261 atgttaatca gggggccgga tatcttcagt ttgctgatga ttatacagta acaacgagca
     1321 atgacagttc atggtccgga ggaggcatta ttgtcaatta tggtacaact gtaaaatggg
     1381 gtataaacgg ggtttccgga gatgaccttc ataaagttgg ggatggaacg cttattatta
     1441 atggcacagg gaaaaatgaa gggggactga agattggggc tggtactgta atccttgaac
     1501 aaaaagcgaa aaacaacgat tcaacggctt tttcttccat taacatcagt gggggaaact
     1561 cgcgtgttaa actctcagga gataatcaga ttatacctga taatgtttcc tggggattcc
     1621 ggggtggata tttagatata aacggaaaaa acacagagtt ctcccgctta caggctgttg
     1681 attatggggc tgcaattatt aacagcagca cagacaagtc attattgact ttaaatctgt
     1741 caccattgaa aaaagatgaa atagctgtaa gcgtgaaggc gttagatatg aacgccatct
     1801 ttcagggggg acacgggaca gccggagatt tgtataaaac aactttttac ggtccgacac
     1861 agtattatct gctgaaaaag cccaaatttg gcagtgtgct gatggggtca ctgaaaaaca
     1921 ccagcgaatg gcaatttgca ggaacagacc tgaatcaggc cgttgatatg gcaaagaata
     1981 ataaactgac atcaagtgca caggcttcat atctttatca cggtaagctc ctggggaata
     2041 tggatattgt cattccggag cttacaggca atgatatttt aacgcttgat ggttctgtca
     2101 gtatatcagg agatatgtca aaacaggacg gtgctcttat cttccaggga cacccggtta
     2161 ttcatgcagg gcaaactgtt tctgcatcgc agagtgactg ggagaacagg gagttctcac
     2221 tcaacaatct gaatcttaat aatgcggact tcagtctgtc ccgtaatgca tttatgaacg
     2281 ggaatatcag ggccgttaac cagagcactg ttattatcgg cggagataca gtctttactg
     2341 ataaaaatga cggaacaggt aatgatgtca tcagtgttga agggaaatct gctgccgcag
     2401 gaacatcctc ctatacaggg catatcactc tggagcaaaa atcagcactg gatatccgcg
     2461 ataattttcg tggcggggtt acgtctgaag acagtcatat caatgtttct tcatcttcag
     2521 tcctgttctc agatgcatcg tcatttataa acagctccct gaatattcat aaaggaggag
     2581 cgctgaccgc tcagggaggg ctgtttacaa gtggaagcat tgatattggt gacgcttccc
     2641 ttctgcttac cggtacacca gtgaattcag atgatgctgc ttttttaccg accatcaata
     2701 tggctgatgg cggatttaac ctgatgtctg attcatcagt actgaaagcc agagaccagg
     2761 catctgttgt tggtgatatt atttctgata aacaggccac aatcagcttc ggaactgaat
     2821 caggtaaaga gggcatattg tctgagaagg catcccgggg actggcggtc ggattactga
     2881 gtggttttaa tacggcatac cgcggtgcaa ttcatgcccc gtctgcatct gccactgtga
     2941 acaatacctg gtggcaactg acaggagact cctcacttcg ctcgttaaaa aataccggaa
     3001 gcatgacata ttttacagga agtgcagcga ataaagcatt ccatacactg acggttgatg
     3061 agctgacgac gaatggcact gcgtatgcca tgcgtacgga cctgaaaaat gcggataagc
     3121 tggtagtaaa caaaaagctg tcaggtaagg acaatattct gttggttgat tttctgaaca
     3181 aaccctccgg agaaaaactg gatattgaac tggtgagtgc accggggaac agcagtaaag
     3241 atgtctttaa aggaagtgaa caggcgatag gtttcagtaa tgtaacgccg gtcattacaa
     3301 cccgggaaac cgatgacaaa ataacatggt cactgacagg ctataacacg gttgcaaaca
     3361 aggaagcaac ccggaatgcc gccgccctgt tctctgttga ctataaagcg tttctgaacg
     3421 aggtcaacaa cctgaacaaa cgtatggggg acctgcgtga catcaacggc gaagccggtg
     3481 catgggcacg catcatgagc ggtaccggct ctgccagtgg tggtttcagt gacaactaca
     3541 cgcacgttca ggtcggggtc gacaaaaaac atgagctgga cggactggat ttgtttaccg
     3601 gtttcactgt cacacacact gacagcagtg cctccgccga tgttttcagt ggtaaaacga
     3661 agtctgtggg ggctggcctg tatgcttccg ccatgtttga ttccggtgcc tatatcgacc
     3721 tgattggcaa gtatgttcac catgataatg agtacactgc aacctttgcc ggactcggaa
     3781 cccgtgatta cagcacgcat tcatggtatg ccggtgcaga agcgggctac cgctatcatg
     3841 tcactgagga tgcctggatt gagccacagg ctgagctggt ttacggttct gtatccggta
     3901 aacagtttgc atggaaggac cagggaatgc atctgtccat gaaggacaag gactacaatc
     3961 cgctgattgg ccgaactggt gtggatgtgg gtaaatcctt ctctggtaag gactggaaag
     4021 tgacagcccg tgccggtctg ggctaccagt tcgacctgct ggctaacggc gaaaccgtat
     4081 tgcgggatgc atctggtgaa aaacgcatca aaggtgaaaa ggacagccgt atgctgatgt
     4141 ccgttggcct gaatgcagaa atcagggata acgtccgctt tggactggag tttgagaaat
     4201 ccgcctttgg taagtacaac gttgataatg cagtcaacgc taacttccgt tactcgttct
     4261 gatactccgt attcaaaagg gcctgcgggc cctttacgct cagtgcctgc cgccctgata
     4321 tgtctgagag acatttccgg gatgtctgtc gactggttta cttaactgcg atggttaaag
     4381 ctggcagtaa tgccggcttt tttacggtcg cacacgtctt cctctcatcc ggaactgtga
     4441 tacaaatcct gcatacagga ttgaccgccg gttcctgcaa atggcacatt ttcagtaaga
     4501 tagcgaaggt tacattcccc cgtcatgtgt ggcacagctg tcaccgggaa agaaacggaa
     4561 agcacatgaa aaacatcaaa actcacaccg gactccttat ccacaaagaa caaacgcggc
     4621 gcgttcgtct tcatgaaaca cccactgcct ggtgccacac gcaccgggaa tgctacagca
     4681 aaacgaccgg tcgccgctgc ggcagccctg acagtctcag caggctgatt ctgagcagca
     4741 ttcagcccat agaatgagat aacccctatg aatgatggca gacagacgcc tccgcccccc
     4801 gaggcatgga aaatccggtt tctggcgaac ggtacacccg gcacatggtt ttacaccgat
     4861 gacgcagccg tcacaaaaga atggctgcgg gcacattgtg acgatgtgga agtgatcccc
     4921 ctgtgccgta tgcatgacgg tcccgttttc cgggaacacc atataaatga actcagcgcc
     4981 gcactggcca gaatgctcca tatgcaccac atactgctgg aacgaacccg gtacagagaa
     5041 aatatccatg atgcagaatg tattcgtgaa atccacgaag cccccctgca ggctatcggc
     5101 atcctggaaa aatacaaacg cacaaaataa taaggctgtt ctatgtggat cctgattttc
     5161 tggctctcat ccccctttaa tgtctcctcc ggcgatgccc ggcaacccac actggctgtt
     5221 cacagtcagg cgttccgtac tgaaaaagcc tgtcgggatg ccttcaccgc gctgaaaaaa
     5281 gaaaacgtgg gggaatttgc tctccgtggc gtctgcgtcc ccgccggaga ataaacaaga
     5341 catgcgcaac cggagtccgc tatgacatcc ccggacctta tacaagctga tctgcaccga
     5401 atgtcctggt ctcagctggc aaaagccgcg gaagagtcga cagtacatca tgattatgcc
     5461 cgggcgttaa tattgtggcg gcatgcatat catgccgcca cgctgactat taataaaaac
     5521 ctggcaaccg ctaaaatcaa cttctgtgct aagcgcattc tgatgcgtaa tcaaatgagc
     5581 aaaataatca gacatacaga cacagacgaa agactcttcc gtctgagtaa acaccatcat
     5641 ttatatgaga aaaagaaaac aaaagaggga tgacaatccc atgatatggg aaaaacttgc
     5701 catgcttact gcaggtgcat ttttgatact ggcagtcctg tgggggtaat aatatcctgt
     5761 ttattaaagg gcagaaggcg gtgcccctct gcccttaagt acgtttccgg gattatcagt
     5821 caataagttc ccggaacagt tccagaagag ctatcactgc attgaccaca gcaacaactg
     5881 ccctgagaac gatgattaac aggttaatca tccgtttctc cgtcaatgga ggaaatcact
     5941 gttgtttact cttacattgc ctgtcacaac agctctttca ttgttagatg taaagtgatt
     6001 tctctccagc cagagcactg aacacagcac cacctgttcg atcagccagg acttaaaatc
     6061 tcgcgttcat cgtcccattg gagatcacgg cagaagcatc accagacaga gctaacggag
     6121 cgattatagt catttttctg attgcaaaaa agagaagcgc agcctactat gatggtgtta
     6181 gatgtagagt gatttccctt tcggtcagag tcaccaactc cgccccggaa tcgcaaaaaa
     6241 accgacctca ccagtcggtt tttttgtgcc tgtctctcac tgccttctgc tgttcctgtg
     6301 aaaataccct ggttatttca tccctgcccg gtcattaccg gcgattgttc gcattaattc
     6361 cactcagcct catcccgaaa tttatctatt tatcgatgga ggaatccccg tgtcttacca
     6421 acagacgtct gccgccgaag atccaatggc tatatggtat atcgtcggtg ccatatgcct
     6481 gctctttgcg attatcatct ggcgcttttt acctgaaatc gtcttcgcct cctgcctgat
     6541 cctgcacaca ctgtggggca tgattgactg ggggcccttt cacaactttg ccgccccccg
     6601 ttacaacctg ctggccatca ctgctaacaa cgcggcaacc atcactttca gccagtggct
     6661 ggatgttatg tcccggacag tggggatcct ctggttgatt ctgcttccca tgacattcgg
     6721 ctttctgtgg atgtggtttc accatccggc ccagccccgt tttacccgca ggccactgaa
     6781 tatccacaca ctgccccata ttttttcagc tctgtcaccg gcgatcgccc ccgttctggc
     6841 tgatggagac aacaacagac tcttccacgg acaaaaacgt cccgagcgcc gtgtcgcact
     6901 aacccccgaa gcctttgtgg agcagaacaa ccttatccgc aacatgcagc tcgatgttgc
     6961 gtccacacgc cagtgcttca tggcgcaact ggggcaacca ctgaccagct ggaaagacat
     7021 ggccccacat gaaaaagcgc tctttgccat ctttggcctg cagttcttcc tcggtgaccg
     7081 gaaagcagcg gtagccctga tgaacaacct caatctctcc tgcaggctca aaagtaaacg
     7141 ggaccagggg cgtttcagca cgccggtgta ttcactggcc agaaacgcct ttatccgggt
     7201 catcaaaaca gaaggcgcac aaaagtggct caggcagcac cgttacgttc gcagcggtct
     7261 ggtctggctt tacgcacatg atttgcgcct gacgcctccg aactggctgt ggctcaaggg
     7321 cgttgaccgg acgctcttct atgcccttca ccgggcaaac accacaaaag gcttcatcga
     7381 aggggccggc gtggtggccg ttgcccgggc agaaaatgaa gcgtgtcgtc tcggcctgcc
     7441 ctgcccggaa ccctgtgtgg aagaagccat tgaagggctg cggcaggaca tgctgagact
     7501 ggggttgata tgggacgaac ctcagccaga ccgcgatcgc agacgtcaga taaggacccg
     7561 ctggtcactc acggatgacg tgataccacg ccgccatgat aacgatgaag gctcagatac
     7621 tggtgaaacc accgaaacca ctgaaaccac tgaaaccact gaaacccggc atcccgccga
     7681 caaggaaaaa gcacaatgac catccgctac actgcaacac tgaacaataa acacctgtcc
     7741 ctgaccgccc tgtaccctga cggtggtcag gaggttattg ccatcctgcc ggttacagaa
     7801 ctgggcgcac tgattaaccg taataacagg ctgatgcacc tgtccttcag cacccctttt
     7861 cgtgaaataa ccgaaacact gcttgttgat accgttgata ctgaacgtcc cggacatcac
     7921 atcggcagaa tgctctccgc gattgacagc tgtctgagcg cggcatatgc agaacttcct
     7981 gcccggaaaa ccagcttcat gagagggctg atatacggtg caggattagt gctggcactt
     8041 ctgacagcgg taacagccgg atatatttta ctgaaaccgg acagcgattc ccctctcagg
     8101 gtacctgtca cagaatccgt aacagaaaca gcaccattac acatcgacaa ggaaattgtc
     8161 cggtcagtag ccccgtcaga gacgaaaaat gaaatcgttc acccggcacc acaggaaatg
     8221 caggagacat ccgctacccc ggcgataaca gcagacaaaa agactctggt cgctgaagcc
     8281 cgccgtaatc tggccactgt acttaaacgc aacgcggacc ggggtatgtt caccattaat
     8341 ctctccactg gtcatgaacg cacactctac gccttccttg accccgcatg catcaactgc
     8401 cgtcttctgg agcccgcgct taagcaactg gcccgggaat tcagcgtggt gatttatccg
     8461 gtctccgtaa tcggcggaga ggccagcaca aaccggactg cgccactgct gtgtgaaccg
     8521 gacgcacaaa aacgggcgac cggatggcat cagctgtatt ccgcagacgg tggtatggca
     8581 cccaccggcg atgcaccggc tgcaccggat gaaaagtgtc tgaccgctgc tcgtgctgcc
     8641 atcgatgtca ataaccaggc tttccgccag ttcggttttg gcggtacccc atgggtgtta
     8701 tccgataccg gctggcacct gccgtccggc atcctgaaag acgccactac cgtcagcctg
     8761 ttcctgaaag ccacagatac cgaggataat cacaatgagc cagcatcacg ttaatcctga
     8821 cctcatacac cgcacggcct ggggaaaccc gctgtggaat gcactgcaca acctgaacat
     8881 cattggtctg tgtctggccg gcagtatcat tacggccctg atatggccac tggcccttcc
     8941 tgtctgtctg ctgttcaccc tggtcacctc cgtgatattc acgctgcaac gctggcgatg
     9001 ccccctgcgt atgcccatga cgctcagcct tgatgacccc tcccaggaca gaaaggtcag
     9061 gcgaagcctc ttcagcttct ggcccaccct gtttcagtat gaagcagatg aaacatcccc
     9121 cgcacggggg attttttatg tcggttaccg ccgtatcaat gatatcggtc gtgagctctg
     9181 gctgagcatg gatgacctca cccggcatgt catgtttttt gccaccaccg gtggcggtaa
     9241 aacagaaacc acctttgcct ggctgctcaa cccgctcagc tggggaagag gctttacctt
     9301 tgttgacgga aaggcacaga acgataccac gaggacaatc tggtatctct cccgacgttt
     9361 tggccgggag gatgatatcg aggtcatcaa cttcatgaac ggcggaaaat cccgcagtga
     9421 aatcattcag agcggcgaaa aatcccgccc acagtcaaac acctggaacc cgttcgcatt
     9481 cagcacagaa gccttcaccg ccgaaaccat gcagtccatg cttccccaga acgttcaggg
     9541 gggagaatgg cagtcacggg cgatagccat gaacaaagcc ctggtctttg gcacaaaatt
     9601 ctggtgcgtc cgggaaagaa aaaccatgtc cctgcagatg ttacgggagc atatgacgct
     9661 ggaaggtatg gcaaaactct attgccgtgg gcttgatgac cagtggccgg aagaagccat
     9721 cgcaccactg agaaattacc tgcaggacgt tccgggattc gatatgtcac tggtgagaac
     9781 gccgtcagca tggactgagg agccccgtaa acagcatgct tatctctccg gtcagttctc
     9841 ggaaacgttc acaacctttg ccgaaacctt cggggacgtc tttgcggcag atgccggcga
     9901 catcgatatt cgcgacagta ttcacagcga ccgcattctg attgtgatga tccctgcact
     9961 ggacacatcg gcccatacca cgtcggcact cggtcgtatg ttcgtcaccc agcagagcat
    10021 gatcctcgcc cgtgacctgg gctaccgcct ggaaggcaca gatgcacaga ccctggaagt
    10081 caaaaaatac aaaggcagct tcccctatat ctgtatcctt gacgaagtcg gtgcctacta
    10141 cacagaccgc attgctgtgg aggccaccca ggtacgttca ctggagtttt ccctgataat
    10201 gatggcgcag gatcaggagc gaattgaagg gcagacctcc gccaccaaca ccgccacact
    10261 gatgcagaac gccggcacca aattcgccgg acgcattgtc agtgacgaca aaacggcaag
    10321 aacggtgaaa aatgccgccg gtgaagaggc tcgggccaga atgggcagcc tgcagcgcca
    10381 cgatggtgtg atgggtgagt cctgggtgga cggtaaccag atcaccatcc agatggaaag
    10441 caaaatcgac gtgcaggatt tgatcagact gaatgccggt gaattcttta ccgttttcca
    10501 gggggatgtg gtgccgtcag cctctgtgta tattccggac agcgagaaaa gttgcgacag
    10561 tgatccggtg gtcatcaacc gctatatttc cgttgaagcc ccccggctgg agcgtctccg
    10621 ccgtctcgtc cccaggacag tgcagcgcag gctccccaca ccggagcatg tcagcagcat
    10681 catcggtgta ctgacagcaa aaccatcccg taaacgtcgt aaaaaccgta cagagcctta
    10741 ccgtatcatt gatacattcc agcgccggtt ggcaacagcg cagacatcac tggatctgct
    10801 tccgcaatat gatacagata tcgaatcccg ggcaaacgaa ctgtggaaaa aggctgtgca
    10861 cactatcaat aacacaacca gggaagaacg ccgggtatgt tatatcacac tgaatcgtcc
    10921 tgaagaggaa cactccggtc ctgaagatat accttcagtg aaggcgattc tgaatactct
    10981 gctccccctg gagatgctgt tacctgtacc ggatctaact gcatccccgc cccataaaaa
    11041 aaatgtcgct cagacaccat caggtggaaa acaagagagt cgtaaaaaaa gattctgata
    11101 acacctgtat ggctgatttt aataacaacc ttaataacaa cctttacgcg attattaatc
    11161 tcctccgctc accatagtaa gcggaggatt caggatttca atccatcatc agaggtacaa
    11221 taaatgtgta ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc
    11281 ggagaagata tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggata
    11341 ctgatggtgc ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga
    11401 tgggacagag aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc
    11461 gaaatactgg gtaagctccc cataataaat aaagtcacaa cactgataaa atcataatct
    11521 actgatttta ctcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga
    11581 aaacaaatat gggcagcaca aaaaatggtg ccagggttcc ccacgttctt aatagattca
    11641 cttctatgga tattgatatg aattcccaga tcctgccaga catgctgatt aattttgccc
    11701 ttattaacat cacagacaga aagaacgagg gtaccaatac cattgatggt aactggcagg
    11761 ccgatgaagg acgccgttac cgggataatg taagaatcta tttttgagta tgtcaacata
    11821 acctgaggca aagtacctca ggttccagtg tagtaatcag ccaacctgac agtcttctgt
    11881 tagccctaca gatatgtcgt ggagctaacc gcgcatgtca aagccggatt tttgaaaatc
    11941 tctataaact tccggattgt tgaaggccgg gaatttagct ttatgggctg ctgactttat
    12001 cgcttcgcaa taggctttat cgccattact ggttgatatt tttaacgccg tgccatcccg
    12061 ggagaattcc atatgcaacc tgcatttttt ccttttccat ttttgcggat catcaagttt
    12121 agcattaatt gcatccctga ttccccgtgc ctgctccccc cattcatcct gatcatccca
    12181 acgtcctgaa ctgcaactac ccagggctgt agttttatgg caatttgaag gatgtaatgg
    12241 cgcacatccc gccacaaagc tgactaaaat agccagcata acgattttct ttacccccat
    12301 atattgctcc tcaatccata tagatctcag taccatagct ctgtcagagc aaagaaaatc
    12361 ttaactaata caagttaaga tttttaatat ccactcctca tccctacatt ccaggtgatg
    12421 gtggcgtcca ctcattatcc tgaactggag cataagatgc gccatgcccc ccggatatgc
    12481 ttttctgttg cctgatatat tcgtggtagt actgttcact acgctgacga tgtaaatcca
    12541 catccgggcg gctgattaca aagtgtccat ttccggttat tccagctgca ttatgccagg
    12601 ccaccatttc cgcaaataca tcattaaact gttgatatga gccttccggt tcaaaaccca
    12661 ttcctggctc acgatcaaac aatacaggag caatcagatt cagcttatcc accagttcat
    12721 ttctgtcctg atgatggtag aacaccacac ggtccccata atccacacag actaactcgc
    12781 cattcctccg atcacgaaaa cgcacactgc catctttctg cagacgggct tcgagaacgc
    12841 cagtgtcttc ataaagtgga gttcctcccg gttcacagag aatcacgcag cggtcggcat
    12901 tcgttgttcg tcgcttgtct ttgcgacgat cacgataatc ccagccacgt aactgtgata
    12961 atgcagctct gtctccctgt accgcctgtt gctccaccca ctgccggtag gacaaaggat
    13021 accacttacc ctccgcaatc agtgacaacc gctcttcctt cacactctcc ttcagcctga
    13081 tcagcgcctg catacgctgc acttccgcaa tatggtaatg cagcttacgc aattgtggat
    13141 cccggaactg cacacggata tacgccttgc gccgacggca ggcagcatga atttcccgta
    13201 accgttctcc gtaacgtaag tcgggtttac gccagtgctc cttccataca agataacgcg
    13261 cgcgcaaatc ttcccgcgca gctgcacggg cttcacggcg ttcacgacgc agtacaggat
    13321 cgcgccggaa gccgggctcc acttcatcgc tggcagcaag ttccggattg tagcgacatt
    13381 caggcttcac ccgttcaaat atatctgcac ctgcaatctc aaatggccct gcctgcggct
    13441 cagccctgga taacgtcaaa tccgggtgga tactgctggc cttcactggt gtgagctcat
    13501 gattaaacgc atccacaatc acaagaccat gatgctgttt ctgcaacata agccctttac
    13561 gcgcaaacat ctcatggcac gcctgccagc tgaaattccc gcccgcgatc atatcttcaa
    13621 tcaacgactc acactgaaca gtcgtgtacc cccacagact ttccttatca gaaaacagtt
    13681 cgcgctttat tgctccctgt ggacctgtcc ccatccgcat acgataccat tcgtaatcac
    13741 tgacagcctg agtaccaaga ttacgtcccc ggaatcgttc tccgggtata acctgagtaa
    13801 aaatatcctt cggtacaggc tgccatccac catccagatc ctgcatataa tccagcgtca
    13861 ttaacggcca cacactgtct gcacgtactg gtgtctgttg tttatcaaat ccatcatgca
    13921 gtaccagatg cccgtgctgg atccccatcc ataatccagc ctttgccaga gttctgtgca
    13981 catccaggca actttccagc tcaccatttc tcgccatttc cggtaagcgt tcggcaaaat
    14041 gacggcaggc gtactgtttc agactctccg tttctgccac tttttccggc cggatatcaa
    14101 cgtttatggc ttccggatca taacgaccgg gtgtccccac ctgactgaaa atatctgtcg
    14161 gtacaggctg atattccccc agtttccgcc ccagtttttc agctgtccat gacggtccga
    14221 acgaactgag tgcaacacct tcccgcgctc tgtcccagcc atccttcacc accagttccc
    14281 cttcctgcat cgtgatatac agaccatcac tggccagccg tttatgcaaa gacaaccagt
    14341 cttgtgcggg ttcttcacgc agcccggcaa tcgacatctg cgcaatatat tccctgaacg
    14401 tctgcttttt cccgcgtcgc caggcattac ggcgctcacg taccagtgct gttttgcgca
    14461 caatacggtt gccgggtgca tgcacaaagc agccattatc cggcgcgaat ccgtgcttaa
    14521 gttcaagttc acgacatgca cggctgagtt tttcctggct ccaggaaagc cagttaatat
    14581 aaccagtttc aggatgtaca cgattcacgg caacatgcac atgcagatta tccgtgtccg
    14641 tatgaacggc agccacgtac tgatgttttg agagttcaag agatttcagt gtgtgacgca
    14701 cacagtcaaa aagctgctca ggacgcgggc tttcatgcgc aggccaggaa agaatgtagt
    14761 ggaaaacggg gtctgtatca ttacgggaaa aaacagcttt gtccgccttg tactgcatct
    14821 cttcagcagc tgtttccagt gaattgcagt tatggaaaca ggttacgcca tagaaattta
    14881 cccactgact gccgtctttc atcacatcaa tcagggagac aaatttttcg ttacgcaggc
    14941 gtgttgcata atcaacaagc cgtctgaaac ggttgcggtg agggacatca ctcttgattt
    15001 cgctgtcttc tgtgagctca ttttcctgca catcatcccg cacagacgtg tacgcaatca
    15061 ggtcttcaaa ggaagacctg ccgtcacgtc ttttgtgcgg aataatggca ttcatcagca
    15121 gttatctcag cgtcccatta agtctcttcg cagagcattg acatattcag taatggcaac
    15181 aagaacctct gcatactcct tatcaccagt gcgttttccc tccacaaaca gatgcttctg
    15241 aaggcggcca agacgcataa tctcatcaat catacggtcg tcagtacggc agttaaccac
    15301 cttatccagt gaagagcttc gtataaatgt tgaaaccgtc tgtccacttg actccgcttt
    15361 ttctcttacg atttcagcct catcctccgt gaatctcgcc gtgaatttta caattctctt
    15421 cctgttctca ctgcccgttc gggttttctt ctcactcatg gtctgctcct taccggggtg
    15481 tcggggcaaa gccctgacca ggtggggaat gtctgagtgc gcgtgcgcgg tccgacattc
    15541 ccacatcctg tcccgttttt caggccatta tagcctgtta tttttgtgca tcatagtctt
    15601 atgcagacgg cagaggcagg gcgcagccct gccaggagtc agtttttgat gataaaatca
    15661 gacttcaaca tgaaacggag agaaagtgcc aatggcttct aaaaaatttt actcagatga
    15721 tgacatccag ctggcaaaag ctgctttatc agaattgccg gacctcaccg ctcaacggaa
    15781 aacattgcgt gattttcttg atgccattcg tgatgacatt attgtcctgg taaggacaaa
    15841 gggttataca ctggcagatg ttcgtgacac gctcaaaaat gcagggtatg aagtaggaga
    15901 gaaggcactt cgtgacatta ttcgggaagc agaatcgaaa aaaccatccc gccgttcttc
    15961 ctctaaaaca gcacctaaaa agacagattc aggcaggaat gatggcatcg atatgaacaa
    16021 caattgatgt gacaatgtca catcgtattc tgagattatg atgattacag agaaaaagcc
    16081 ccggcataaa taccagggct tttctgttta atcagactcg tggcggcaat gcaccaggat
    16141 aatgccgtgc aataatatca ttcagttttt caacaaggtc aggacgctta aagacaatgt
    16201 gtacggttcc tttctggtag ccacgcaccg taaaatattc cccttctaac agcttaccga
    16261 cattgtctcc gttcccgaga aattcaccat actgcgcacc gatgctgctc cggaagtcag
    16321 gaacgttgcg cccctccagc agataaaacg gacgtaccag gtcatccagt gcctcccgtc
    16381 cgctgcagtc cagcgtcaca cgcccgttac tccagcggta cagcaggttt tcaatgatga
    16441 ttttcttgcc cagacggcag ggattattgg ttctgtaatc ccatgacaat ttccgccaga
    16501 catcaatcag tccctgttca aatgtctgca tcttgctggc attcagatgg cggaatgttg
    16561 ccagtacatt gtccagggtg atttcaggac aggtgtctct catcagctgg ctgttccact
    16621 catcacgctg acatgaactc atgaaggtgt acatccccgt atccgtcatc aggcggtccc
    16681 agattttacg gtcggcaaga cgggtgatat tccttttcgc ttcttccggt ttatccatgc
    16741 accgaacgga gtcggtcacg cagtcttcaa aaccgtaaag aaaaatatca cctgacgcat
    16801 caagcagaat cctgcgcgct gtcgccaggc attccatcgc ctgcatgaac agtgcaatac
    16861 cttcgttacg ctgcgcgatg atacggtcga tagccacaga cggaataaca tctgtcgtgc
    16921 cggcagtatt caggtcagca gtgggtaaag tctgattcat cacattgtct cctcatcatc
    16981 acggctgccg ggtcagcccg gtcatttccc ggaggagcga agctccttct gccgtccggg
    17041 tcttcgggga t
//
LOCUS       DAZUFB010000104        16734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZUFB010000104 DAZUFB010000000
VERSION     DAZUFB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.104"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..215)
                     /locus_tag="ACHY3M_003845"
     CDS             complement(63..215)
                     /locus_tag="ACHY3M_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4318670.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(480..1214)
                     /gene="cysH"
                     /locus_tag="ACHY3M_003846"
     CDS             complement(480..1214)
                     /gene="cysH"
                     /locus_tag="ACHY3M_003846"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS4318671.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1288..3000)
                     /gene="cysI"
                     /locus_tag="ACHY3M_003847"
     CDS             complement(1288..3000)
                     /gene="cysI"
                     /locus_tag="ACHY3M_003847"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFS4318672.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3000..4799)
                     /gene="cysJ"
                     /locus_tag="ACHY3M_003848"
     CDS             complement(3000..4799)
                     /gene="cysJ"
                     /locus_tag="ACHY3M_003848"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFS4318673.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEKAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5118..5480
                     /gene="queD"
                     /locus_tag="ACHY3M_003849"
     CDS             5118..5480
                     /gene="queD"
                     /locus_tag="ACHY3M_003849"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFS4318674.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5558..6829
                     /gene="ygcN"
                     /locus_tag="ACHY3M_003850"
     CDS             5558..6829
                     /gene="ygcN"
                     /locus_tag="ACHY3M_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4318675.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6820..7080
                     /gene="ygcO"
                     /locus_tag="ACHY3M_003851"
     CDS             6820..7080
                     /gene="ygcO"
                     /locus_tag="ACHY3M_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS4318676.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7097..7672
                     /gene="ygcP"
                     /locus_tag="ACHY3M_003852"
     CDS             7097..7672
                     /gene="ygcP"
                     /locus_tag="ACHY3M_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFS4318677.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7820..8680)
                     /gene="ygcQ"
                     /locus_tag="ACHY3M_003853"
     CDS             complement(7820..8680)
                     /gene="ygcQ"
                     /locus_tag="ACHY3M_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS4318678.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8677..9456)
                     /gene="ygcR"
                     /locus_tag="ACHY3M_003854"
     CDS             complement(8677..9456)
                     /gene="ygcR"
                     /locus_tag="ACHY3M_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFS4318679.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9434..10843)
                     /gene="ygcS"
                     /locus_tag="ACHY3M_003855"
     CDS             complement(9434..10843)
                     /gene="ygcS"
                     /locus_tag="ACHY3M_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318680.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10865..12319)
                     /gene="ygcU"
                     /locus_tag="ACHY3M_003856"
     CDS             complement(10865..12319)
                     /gene="ygcU"
                     /locus_tag="ACHY3M_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFS4318681.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12389..13174)
                     /gene="ygcW"
                     /locus_tag="ACHY3M_003857"
     CDS             complement(12389..13174)
                     /gene="ygcW"
                     /locus_tag="ACHY3M_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4318682.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDSMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13493..14770
                     /gene="yqcE"
                     /locus_tag="ACHY3M_003858"
     CDS             13493..14770
                     /gene="yqcE"
                     /locus_tag="ACHY3M_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFS4318683.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14796..16274
                     /gene="ygcE"
                     /locus_tag="ACHY3M_003859"
     CDS             14796..16274
                     /gene="ygcE"
                     /locus_tag="ACHY3M_003859"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS4318684.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         4020 a         4374 c         4193 g         4147 t
ORIGIN      
        1 aaagggtgca ggagaccaaa ccatcacaat tcagtacgcg gggttttacc cccgcgtggt
       61 ggctacttct tcgattcgta agcgagaact gccttaaact ccataccacg ttctctgata
      121 ctcaactcac acaacgagtc acctaccatc agcgtgaatc caagtactgt acaacacagt
      181 acgatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc aaagaagaga
      241 ctaacattcg aattgtttgg gttcgaacgt aagcctcagg ttgatagaaa tatcgcctgg
      301 ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca agcacccgac
      361 gccattctat acctgataat tcttccagcg gcgttcacgt ttaaaatcag gaatactgct
      421 cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc cggcaatatt
      481 tacccttcgt gcaatccaca ttcccttttt aagccaaaga aacgtgtttc ttcttccgac
      541 atgccaggtt cccatttacg ggttgtatgg gtatcgccca ccgataaata tccttcgtcc
      601 cataatgggt gatatttcag gccatgtttt tgcagatact gatatatcgt tcggttatcc
      661 cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac cggtaaattg
      721 gcgcggctgc cggattgttc gcggcgcagg ccagcaaacc acgtttgcgc attcagttct
      781 ttcagcgccc ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc aatgccttca
      841 acgccctgtt cccacagttt gccgtagcgt gcttcctgcc aggcggcgct ttcggtagcg
      901 cggtacactt tcaggttgag cttgagtttg tccgttaact cgtcaataaa gcggtaggtt
      961 tccgggaaca gatagccggt gtcggtgagg atcaccggaa tatccgggtg aatctgattc
     1021 accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac atattcaccg
     1081 ggcagattat ccagcgccca ggcaacgcgg ccttcagcgt ccagtttttc cagttcggcg
     1141 ttagtttctg ccagcgccag aatgcgatcg accttcggca gttcgttcag ggcgtttaga
     1201 tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct cacgcgtctt
     1261 atcaggccta caagaccggg ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac
     1321 cgggcgaatg atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc
     1381 tttcgcccag cgccctatca gttcatcaag cgacgccagg atttccggct cggtgatgtt
     1441 ttctttatac atccgtggga tacgtgtccc aatgcggttg ccgccaagat gcaggttgta
     1501 gcgacccggc gctttaccca ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt
     1561 cgggcagcct gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa
     1621 attatcgatg ttgtcgataa aagacggcag gaaacgctct gcttccgcca tcgccagcgg
     1681 gcaagtcggg aacgagacgc aggccatcga gttttcacgc tgcggcgtga cggcattcat
     1741 taacccgctc tctttggcga tcttctcgat cttcgctttc tcgctttccg gtacaccggc
     1801 gatgatcaga ttctggttcg ccgtaatgcg gaaatcgcct ttgtggatct tcgcgatctc
     1861 cagcaggccg gttttcagcg gacgccccgg ataatcaagg atgcgaccat tttcgataaa
     1921 cagcgtcagg tgccagttat catcaatgcc cttaacccag ccaatacgat cgcctcgtcc
     1981 ggtgaactca tatggacgga tcggttcaaa tttgatcccc gcgcgacgct ccacttccgc
     2041 tttaaacgtc tcaaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc
     2101 ggttcggtta ccccagtcac gctgagttgt cacgacggct tcggccaccg ccagcgtatg
     2161 ctccagcggc agatagccaa actcgctcgc cgtgcgggcg taggttttct tgttgccgtg
     2221 ttcgatagaa agcccaccgc ccaccagcag gttgaagcct accagcttgc cgttttcggc
     2281 gattgccacg aagttcatgt cgttggcatg cagatcgata tcgttctgcg gcgggatcac
     2341 taccgtggtt ttgaacttac gcggcaggta ggtctgacca aggatcggtt cttcatcagt
     2401 agtggcgact ttttcgtggt cgagccagat ctccgcatac gcgcgggtac gaggcaacag
     2461 atgctcagaa atcttcttcg cccactcgta cgcttccgcg tgcagctgcg actcgtaagg
     2521 gttcgaggtg cagagtacgt tacggttcat gtcgttagct gtcgccagcg catcaagacc
     2581 gaccgagtgc agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg
     2641 aaacgtctgg cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt
     2701 gtcgatcgcc tgccactgtt tagtggtaat caccccaccc ggcagacgac agcgaagcag
     2761 catcgcgtgg cgcggctcca gcttctgttc agcacgttcg gcgcggatgt cgcggtcatc
     2821 ctgctgatac atgccgtgga agcggatcag caggaagttg tcgcccttaa agccgccggt
     2881 cagaccgtcg tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat
     2941 gcgctcggcg tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat
     3001 tagtagacat ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc
     3061 gcttcggtgt ccatgccacc aaattcggca atcacttcca gaagtgcctt ctcaacgtct
     3121 ttcgccatgc gattagcgtc gccgcagaca taaatgtggg caccatcatt gatccagcgc
     3181 cacagctccg cgccctgttc gcgcagtttg tcttgtacgt aaactttttc tttttggtcg
     3241 cgcgaccagg caagatcgat acgtgtcagc acgccatctt tgacgtagcg ctgccactcc
     3301 acctgataca ggaagtcttc cgtaaagtgc ggattaccaa agaacagcca gtttttacct
     3361 ggcgcttcgt cggcggcgcg ttgctgcata aaggcgcgga acggcgcaat accggtgcct
     3421 gggccaatca taatcaccgg ggtttccggg ttagcgggca ggcgaaagtt atcgttatgt
     3481 tcgataaata cgcggacttc gccctcttct tccacgcgat ccgcgaggaa gctggaggca
     3541 ccaccggcac gggctcggcc ttccacgtcg taacgcacca caccaacggt gacgtgtact
     3601 tcgttctcga cttccgcctg cgaggaggcg atggaataca ggcgcggggt cagcgggcgc
     3661 agcagattaa tcagcgcttc ggcatccagt tgcgccggag agaaacgcac catgtcgaca
     3721 atcggcgtcg tcgcggcgta atgctgtaac ttcgctttat cgcccaccag cggcagcagt
     3781 gtttcgctgc gggtaagcgt ggcgtaattc tcaacaatgt tggcggtgtt gacggtcagt
     3841 tcgaagtgcc actgtagcgc ttcgttcaga ggcaacgttt tgccctcgac ggtgacaggt
     3901 tcatcgcctt tcagccacag cagttcgaca agttctttca ccagtgccgg atcgttctga
     3961 taccagacgc ccagcgcgtc acccggctgg taacgcaggc ccgagtcacc taagtcaatt
     4021 tcgatatggc gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aacagagagg
     4081 ctagccacca gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt taccgcgcca
     4141 gtagcgacgg attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg
     4201 cgggcgcgcc actcgctggc agcagcctgg tattcaacat cggcatcgac acggtcgagc
     4261 aggcgttcac cacccagttc cgccagcttg ctgtcgaaat ctttcccgga ctggcagaaa
     4321 aattcataag agctatcgcc gaggctaaac acggcaaacg cggtgttttc cagctttggc
     4381 gcttttttgg agaacaggaa cttatgcagc gcgacggctt cttccggcgg ttccccttcc
     4441 ccttgcgttg acgtcactac gatgagcagt ttttcgctgg cgatttgttt gaatttatag
     4501 tcgcccgcgt tcaccagctt aacgttcagt tttgctgcta ataaatcatc gcgtaatgct
     4561 tcagcaaccc ggcgcgcatt gccggtttgc gaggcggaga taatagttat acccggcatt
     4621 tctgcggctg gcgctggcgt cgctgcaagc gcagcaggct gctgattgag cacgccccag
     4681 aaatagccag aaacccaggc aagctgggtg ggagttaaat cggtcgtggc cgcctgaagg
     4741 cgtgccagtt gctccgggtt caacggaagc aacgcggaag gtgggacctg tgtcgtcatg
     4801 cgtcgttatg ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga
     4861 agagaaggta aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa
     4921 taaccaaatc gactaacctg ttttagcaat agtctttaac aacaaaatag attaaccaac
     4981 ctaatgaaaa acaaatgaat ttagccaatc attaagataa atcagcgatt ttgcgcaaca
     5041 aagtcgtttt agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt
     5101 cctgtagaga aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc
     5161 accgcttacc acacgtcccg gaagggcata aatgtggtcg cctgcacggg cattccttta
     5221 tggtgcgact ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg
     5281 ctgaactaaa agcggcgttt aaaccaacct acgagcgcct cgatcaccat tatctcaatg
     5341 atattccagg tctggaaaac ccaaccagcg aggttttagc aaaatggatt tgggatcagg
     5401 ttaaacccgt tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgt accgcaggtt
     5461 gtatctatcg cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta
     5521 tgttagggtg ttgtgttctg gatatctggg gcatgacatg gaagacgact gcgacattat
     5581 tattattggt gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt
     5641 atccgttttg ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg
     5701 gttatatacc catgcactcg cggaactcct cccgcaattt catctgaccg cgcctcttga
     5761 acgacgcatc actcacgaaa gcctttccct gttaacgccg gatggcgcaa cgacgttttc
     5821 cagcttacag cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct
     5881 ggttgccgaa gccgaagaag aaggtgtcga atgcatcccc ggagcgacgg tggatgcact
     5941 gtatgaagaa aacggcaggg tgtgtggtgt catttgtggt gacgatattc tccgtgcccg
     6001 ttatgtggtg ctggcagaag gtgccaacag cgtcctggct gagcgtcacg ggttagtgac
     6061 tcgtcctgct ggtgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaccatc
     6121 cgctattgaa gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg
     6181 caggatctgt gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc
     6241 gttagggatt gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg caagcgagct
     6301 gctgactcgc tttaaagcgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact
     6361 ggagtatggt gcgcatctgg tgccagaagg tggcttgcac agtatgccgg tgcaatacgc
     6421 cggtaacggc tggctgctgg tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc
     6481 cgtgcgcggc atggatatgg cgctgactgg cgcgcaggcg gcggcacaaa cgctgataag
     6541 cgcctgccag caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca acgtagagcg
     6601 cagcctgcta tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt tgcaacgccc
     6661 tggctggtat cgggcgtggc ctgcgttaat gcaggatatt tcccgcgatt tatgggatca
     6721 gggtgataaa cctgttccac cgctgcgcca gttattctgg cgtcatttac gtcgtcatgg
     6781 cctgtggcat ctggcgggcg atgttatcag gagtctgcga tgtctgtagc ccataatctc
     6841 tggcgcgttg ctgatgcgcc gcacattgtt ccggctgact ccgttgagcg tcagatggta
     6901 caacggttga ttaacgcctg tccggcaggt cttttttcgc tcacaccgga aggtgactta
     6961 cgcgttgatt atcacggctg cctggagtgt ggcacctgcc gtttgctgtg cgacgaatcc
     7021 acactacaac agtggcgcta tccgccttcc ggattcggca tcacctaccg ctttggataa
     7081 ataataagga taatttatgc ccctcttaca cctgctccgc cagaatccgg tgattgctgc
     7141 cgttaaagat aatgccagcc tgcaactggc aatcgactct gaatgccagt ttatttccgt
     7201 gttgtacggc aatatctgca ccattagtaa tatcgttaaa aagattaaga acgccgggaa
     7261 atatgctttt attcatgttg atttgctgga aggcgcgtca aataaagaag tcgtgattca
     7321 gtttttgaaa ctggtgaccg aggcggacgg cattatcagt accaaagcct caatgttgaa
     7381 agcggcaaga gcggaaggtt ttttctgtat tcatcgcctg tttattgttg attcaatttc
     7441 gtttcacaac attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc
     7501 aggctgtatg cccaaagtgc tgggctgggt gacagagaaa atccgccaac cgctgattgc
     7561 cggtgggctg gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgtcgtggc
     7621 gctttccacc acgaataccg gggtctggac gttagcgaaa aaattacttt gacgggataa
     7681 tcgtaaccaa ttgaatttgg tttgatttgt aggccgcacg ccacatccga cattcagcgc
     7741 ctgatgcgac gcttgacgcg tcttatcagg cctacaagtc cccaggccag aaccgtaggc
     7801 aggatcatcc ggcaacacgt tattggcaat ctgcgtgaat gtttgtcacc agtgcctcca
     7861 gcaccacttt ccagtcatca accacgccaa catctgcctg tgaaaatacc gcagcgctgg
     7921 cgtcatgatt aatcgccacc acaaacttgc tattgcgtac gcccgccatt aacgctgctg
     7981 cgccagaagc gccaacaaca atacacacct caggtgccag cagatgtccg gaaataccaa
     8041 tcactttttc agcatcaacg ccaccgttca tcactcgcgc ccgactgtag cccacttcgg
     8101 ctcccagctt ttctgccagc atagcgattt cctggttatc tgcctcaccg ccctgcccca
     8161 ccaccagcac ccgtctggcc tcagctaatg ggtcacgggt aacatttttc agatcctctg
     8221 tgctaaccag ccagtcggga agcgcgccgg ggacaatgtt cagttgctgc ataccagagg
     8281 gcaacgtggc gtttttcact gctcccgcct gacgcgccag ggaaaggcat agcgggcgtt
     8341 tttctgtttg cagcgtcgcc gttagcgcat ttccccagtg ggatttgcgc acgcttacgg
     8401 ttggaatatc taacgaagtc acctggcaga tgcttgcacc atgtaaacgc caggccagcc
     8461 gggtcgacag ttcatcgcca aacgttcccg gcggaaagag aacaacgtcc gcaggcgttc
     8521 gctgccactg ttccaccaac gcatccagca cctgttccgc cactacaggc tgaggttcaa
     8581 tctgccagtg cgccagggta caaccgctga aatcctgcgc cgccagccag cttgctattg
     8641 cggcattctc ctgatttatt gtcacaattg cgatattcat ggctgcatcc tttggcgtaa
     8701 atagtcctgc catagctttt gagctttttc tgctaccgtt tgcccatcaa tcagcgtcgc
     8761 tccgcgtcgt tgctctgctc ttgccagttg cagacactgc atagctggca tttcggcagc
     8821 aacagtttta cggataattt ctgctttccc cgcagccatt cgctggcgca ttcccggtac
     8881 tggcaaggcc acttcaccgc actgacgcac ggcaatcacc gcaggcaagc gaacccggca
     8941 acaacgcaaa ccatgttcaa tacgctgttc gagggtgata aacagcgcgt cgagagtgaa
     9001 acgctccacc tgggtgaagc agggccagcc cagcatttcc gccagcaaaa atggcgtttg
     9061 cccattctgc ccttcgctgc tttggcagcc ggtaattatc agatccagcg gattctgatg
     9121 ctgccattcg gcaatatggc gagcgacaaa ttccggcgca aagcgcagat ctgccgccgt
     9181 ctccagcaat acagcttcct caaacccaag agccatgaga tagcgcagcc agtgcaatgc
     9241 ccgttcatcc cccatgctca acgcggttaa agacatcggc gtaccgtttt ttctctgcgc
     9301 caacagcagc gcggcagcgg cctgttcatc agcaccgagt aaacttcgca gtagcgaaat
     9361 atccggtccg cttttaccct gagccgccgc ctgccactct ttttccgcca gcattccggc
     9421 atccggttcg gctttaaacg ctaacagaat gttcattcgc acctcctaca tttcccgccg
     9481 ccaccagcgg gagggcttta gtttctggtg cccatagcca ggtcacaaca aaaccaacca
     9541 acaacactgt cgccaggagc aataaggtga cttgcattcc ccactgcgcc agcacccacg
     9601 gcagcaggcc agtacttacc gccgcgccaa gtcgactcat ggcggtggca aaaccgacgc
     9661 ccagcgagcg aatgtcagta ggaaaacttt ccgcaggcaa aatgcccacc agattactga
     9721 ctgccgaaat ggtggtgctg aagagaacaa aaagcagcag cgttaatgaa ctgccggaag
     9781 gcaaacaggc cattactacc agcgttgccg ccagcagcaa aaaacttccc agcaaaaatt
     9841 tgcgatgtgc cagcaggtgc gtcagaacta atcccagcag cgcgcccaca attaacaacg
     9901 cattaagcat caggctggca gtcagcgcat cttccagacc aatagtctgg gcgatagttg
     9961 gcagccaggt ataaatcaca aaccatggga ttacgaggca gacaaagaag acgctgttaa
    10021 acgccgtgcg ccgccagtaa cgcgaagaga acaaggtttt gatgtgttta tgggtcgccg
    10081 ttaccacttc atcgcccagt aaaacatggg gaccaaaata gcgatgcacg atagcgtgag
    10141 cttctgcaaa acgcccctgg cgcagtagcc agcgtggtga ttctggcgtt ccccagcgta
    10201 ataacgtaat caacaacgcg ggcagagctg ccgatgccag tagccagcgc caggcctccg
    10261 ggttttcgga aataaagtga tgtccggcaa tacttgccag cacatagcct acggtccaca
    10321 ccacgctgaa tgcgcccagc aaaataccgc gatggcggcg cggggaaaat tcagccagca
    10381 aggtgtgacc tactgaataa tcgcctccca gaccaatgcc aatcaaaatg cgcagtccaa
    10441 taagatgctc tggcgtggtg gcaaaaaatt gtaaaaacga agcaagcgta atcagcaaaa
    10501 agctgaaggt gaagattttt tgccgaccaa tatggtcgga gatccaccca agaaccaggc
    10561 tgccaaggaa caaaccaagg agcgccgagc cgccgatcat tcccgccata aacggcgtca
    10621 gttgcatggc gggcgtaagc tgaataatgg cgtaaccaat gacgccgaga acataaccgt
    10681 cggtcaggtg tgcgccgaaa gtgagcgcag caatgcggca gtgaaaacgg ttaagcggta
    10741 aatcatccat tcgcaccggt gaagtgttca tctttacccc ttcattgtcc ggtaataaga
    10801 acggcttctc gcctgagaag ccgaaaaggc aacgccccgt catcgcggaa cgctgcctga
    10861 cacattattt ttcaatcgga tagatagtac ccgtattcat aatgccatta ggatcgaact
    10921 gctttttcag cccttccagc aacgcccacg cgctgccgtg ttccagtttg ctccagtgaa
    10981 cgcgatgttt accgataccg tggtggtgca ccatcgaacc gccgaggcga atggtttctt
    11041 cacagatgat cttgttgagc ggattgtggt acttgtcgat ttcctcttcc ggcttacagt
    11101 caacgacgtt gtaatcgtag acgaagtaca tgttggtgcc gttctgatag ctatgagagg
    11161 aatgaccgcc cagcatggtg atgtcgtcgg cgtgcgggaa ctcagtacga atacggttaa
    11221 taacgctttc gtagatttcg tggatgcagc tccagcagcc ggacacttca gtggtaaagc
    11281 ccatgttgcc ggttttgagg atctgcacac gttcggcagc cactttatcc ggtccccagt
    11341 tcaggttgtt aaaccaggtt tcgatcagtt tgctgtccac gcgctggcac tgcgggtagc
    11401 gggcaacgat ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg ttaccttcag
    11461 ccataaagat cagcacacat tttccgtctg caaaatgggt gaagtgttgg gtgccatctt
    11521 cagcgtcata caaacgagcg atcgacggac gatacccttc caccatgact tcacgcagga
    11581 tgttgaagcc ggttttcatg tcttccagga tatagccgta gaagaggttg ttttccgggg
    11641 tgaatttaaa gattttcact gttacttcag tgatatagca aagtgcacct tcgttaccga
    11701 tgatgatgtg gcgaatgtcc gggccagccg cgcggcgtgg cacgttttta atgcgtgtga
    11761 cggtgccatc agccaatact gcttccagac caacgaccat atcttcgatt gcgccgtaga
    11821 gtgtggagaa ctgcccgata ctgcgggttg ctaccaggcc gcccatctgc gccagcggct
    11881 ttgactgcgg agaatgcccc gtggtgtaac ctttttcacg caacgcattt tccagcactt
    11941 ccaggggaac gccacattgc gccgtcgcct gcatattctc aatatcaata ttaatgattt
    12001 gattcatggc ggaaccgtcg agcaccacag agttttctac aacagtttcc agcccacctt
    12061 cagtggcgga agcgccggta cgcggcacac cgttaatttt gtgcgcattc ataaaattca
    12121 gcacacggga gacttgttct gtggaaccga gttttacgac cgctgccgga atcggcaaag
    12181 tataaatacc atgaatatcc ggaaatttac gaaaacggtc aatactgttc ttctttaata
    12241 ctgtttcatc ggtaattacg cgatctgcac caacaatttc ctttagttgg tcgacaatcg
    12301 ctgcgcgaga taaagacata gtaattcctt cctgataata aaaatggaat aatcattaat
    12361 aagagaaaac agaaaccacc gttatggatt agcgcactaa ataaccacca tcgaccacta
    12421 ataaatgccc attgacataa tttgaagccg gacttgcgag gaatacagct gcgcccatta
    12481 aatcctgagt atcgccccaa cggtttgccg gaatatgatc aagaacgcgc tgattggttt
    12541 ctggattact gcgtgtcgcc agcgtaatat ctgttgcata atagccaggg gcaataccat
    12601 ttacctgaat attatattga cctagttcat cacaataagc tttggtgaat ccggcaagag
    12661 catgtttagt ggcagaatat gcaggtgacc attgtccacc taagtaagag aacaatgaac
    12721 agatattaat aattttgccg cttttctgcg ggatcataat ttttgcagct tcatagctta
    12781 actcgaatgc ggcggtcagg ttcacatcaa tcatcgaatc ccagtcggca cgaccgaagt
    12841 ccagcacctt attcagctta caaataccgg cattgtttac cagaatatca actgtaccga
    12901 aacgctcaca gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg atatccacct
    12961 gcatgaagtc cacctcaaca ccctgttttt caatcatttc ctttgtttcg ccgttatctt
    13021 tgacgaaact aggaataaag atatttgcgc cagctttggc caacgccatg gcaaatgcct
    13081 ggcctaaacc gctattccca ccggtaacaa ttgcggtttt acctttcagt gagaaaaaat
    13141 ccattgagaa cgcattgaga gattcgattg acatagttag ctccaatttc cgtaaggcaa
    13201 aaaaaaagaa aggtaacttc ccccgcgaga gggaagttac ctttctcatt atctctggta
    13261 actggagata atttttagca taacggcata actgctaatg tgatcgtaat cacagtgtga
    13321 tattcgtatt gtcaaatagt tttaaaaagc agaagcgtga ttactctcac acaacacctc
    13381 tgctttccat gttatttctt attgcaatta ctagagacct gagaaaagta atccacctga
    13441 aagggcggct tacttttctc ttttttttgt ctattacaaa tgggaatacg atatgcaaca
    13501 caactcatat cgccgttgga taaccctcgc tataattagt tttagcggcg gcgttagttt
    13561 tgacctagct tatttacgtt atatttatca aattcccatg gcgaaattta tgggattcag
    13621 caataccgag ataggtttaa taatgagtac ctttggtatt gcggctatta ttctttatgc
    13681 ccccagcggc gttattgccg ataaattttc acaccgcaaa atgattactt ccgcgatgat
    13741 cattaccgga ttactgggtc tgttaatggc aacgtatcca ccgctgtggg taatgctctg
    13801 tattcaggtc gcctttgcga taacgacgat tttaatgctg tggtcggtgt cgattaaagc
    13861 cgcctcgttg ctgggcgatc atagtgagca agggaaaatt atgggctgga tggaagggct
    13921 gcgcggcgtc ggtgtaatgt cgctggcggt gtttaccatg tgggtctttt ctcgctttgc
    13981 accggatgac agcgccagcc tgaaaacagt cattatcatc tacagtgtgg tttacatctt
    14041 gttggggatt ctgtgctggt tttttgttag cgataacaac aacctgcgca gtgccaataa
    14101 cgaagaaaaa cagtcattcc agcttagcga catccttgcc gttttgcgta tcagcaccac
    14161 ctggtattgc agcatggtga tttttggtgt cttcacaatc tacgccattc tgagttactc
    14221 caccaactat ctgaccgaaa tgtacggcat gtcgctggtg gcagcgagct acatggggat
    14281 tgtgatcaac aaaatcttcc gcgcgctgtg cggcccactt ggcggcatta tcaccaccta
    14341 cagtaaagtg aaatccccta cccgcgtgat ccaaatcctt tccgtactcg gcctgctggc
    14401 gttaactgcc ctgctcgtca cgaactctaa cccgcaatcg gtcgcgatgg ggattggcct
    14461 gattttactg ctgggattca cctgttacgc ctcacgcggg ctgtactggg cctgccctgg
    14521 cgaagtgaga acaccgtctt acattatggg caccacggta ggtatttgtt cggtgattgg
    14581 attcctgccg gatgtcttcg tttacccgat tatcggccac tggcaagaca ctctgcccgc
    14641 agcagaagcc taccgcaata tgtggctgat gggaatggcg gcgcttgcca tggtgattgt
    14701 ctttaccttt ttgctgttcc aaaaaattcg tactgctgat agcgcccccg caatggctag
    14761 cagcaagtaa gccaggaaaa ttacaggaga aaatcatgtc gaaaaaatac atcataggga
    14821 ttgatggcgg aagtcagagc acaaaagtgg tgatgtacga tctggaaggt aatgtggttt
    14881 gcgaaggtaa aggcttatta cagccgatgc acacgcctta tgccgatact gcagaacatc
    14941 ctgacgacga tttatgggca tcattatgtt ttgcaggtca cgatttgatg agccagtttg
    15001 ccgggaataa agaagatatt gttggcatcg gcctgggctc catccgttgc tgccgtgcgt
    15061 tattgaaagc cgatggcacg cctgctgcgc cgttgattag ctggcaggat gcacgcgtta
    15121 cacgccctta cgaacatacg aatcctgacg tggcgtatgt cacctctttt tcgggttatc
    15181 tgacgcatcg cttaaccggc gagtttaaag acaatatcgc caactatttt ggtcagtggc
    15241 cggtggatta taagagctgg gcatggagcg aagatgctgc ggtaatggat aagtttaata
    15301 tcccccgtca tatgctgttt gatgtgcaaa tgcctggcac cgtcctcgga catatcacac
    15361 cacaagccgc actggcgaca catttcccgg caggactgcc ggttgtttgt accaccagtg
    15421 ataaaccggt agaagctctg ggggccggat tactggatga tgaaactgcg gtaatttctc
    15481 ttggcactta catcgcattg atgatgaacg gcaaagcact gccgaaagat ccggtggcgt
    15541 actggccgat tatgtcttct attccgcaaa cattgctgta tgaaggttac ggtattcgca
    15601 aaggtatgtg gacggtgagc tggctgcgtg acatgttagg cgagtcgtta attcaggatg
    15661 ccagggcgca ggatctttca ccggaagatt tactcaacaa gaaagcctct agtgtgccac
    15721 ctggctgtaa tgggctgatg acggtgctgg actggctgac caatccgtgg gaaccgtaca
    15781 aacgcgggat tatgatcggc tttgattcca gcatggatta cgcatggata tatcgttcga
    15841 tattggaaag cgtggcgcta acgctgaaga acaattacga caatatgtgt aatgaaatga
    15901 atcactttgc gaagcatgtg atcattactg gcggcggttc gaacagcgat ctgtttatgc
    15961 aaatttttgc cgacgtgttc aaccttccgg cacgccgtaa cgccattaac ggttgtgcaa
    16021 gtctgggagc agcgattaat acagcggtag gtctggggct atacccggat tacgcaacgg
    16081 ctgttgataa gatggttcgc gtgaaagata tctttatacc gattgagagc aatgccaaac
    16141 gctacgacgc gatgaataaa ggcattttca aagacctaac caaacatact gatgtgatcc
    16201 tgaaaaaatc gtatgaagtg atgcatgggg aattggggaa tgtggattcg atccagagct
    16261 ggtcgaatgc gtgaatgagg taggccgggc ggaaaactgc ccggcctgaa catacctaaa
    16321 tggttatccc cgctgacgcg gggaacataa gttcgacaac ttaaaaaatg ataacaagtc
    16381 cggtttatcc ccgctgatgc agggaacata ataaaaactt taactagttc ggtttatccc
    16441 tgtttttaca aggaatatta actacactcg atgtctttag aattaaagac aatacgaatc
    16501 gcactttatc aattttcact taaagtatga actggatatc tttattcata agaactattc
    16561 atcaacagcg tatagaggcg gttatgtaaa accactcatt agcctcaaaa caaagacaaa
    16621 cttccagatt tattacttaa ccaatgaata acaagcttaa aaaatatact gttattctaa
    16681 agaaaaaatt aacaggcatt aattcaatat taaaaaaata atagattaaa attt
//
LOCUS       DAZUFB010000105        16701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZUFB010000105 DAZUFB010000000
VERSION     DAZUFB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.105"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..168
                     /locus_tag="ACHY3M_003860"
     CDS             <1..168
                     /locus_tag="ACHY3M_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFS4318685.1"
                     /translation="HGGERGALLYSLIGMCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(285..1544)
                     /locus_tag="ACHY3M_003861"
     CDS             complement(285..1544)
                     /locus_tag="ACHY3M_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4318686.1"
                     /translation="MALTDAKIRAAKPSDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARR
                     WHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIAS
                     RLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYK
                     GQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKM
                     RTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQ
                     EVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQW
                     WADFLDANREKFISPFEYAKINNPLKQ"
     gene            complement(1742..1817)
                     /locus_tag="ACHY3M_003862"
     tRNA            complement(1742..1817)
                     /locus_tag="ACHY3M_003862"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1782..1784),aa:Phe,seq:gaa)
     gene            complement(1923..2630)
                     /gene="yqgA"
                     /locus_tag="ACHY3M_003863"
     CDS             complement(1923..2630)
                     /gene="yqgA"
                     /locus_tag="ACHY3M_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4318687.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWDAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2783..2866
                     /gene="yqgH"
                     /locus_tag="ACHY3M_003864"
     CDS             2783..2866
                     /gene="yqgH"
                     /locus_tag="ACHY3M_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFS4318688.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            3028..5163
                     /gene="speC"
                     /locus_tag="ACHY3M_003865"
     CDS             3028..5163
                     /gene="speC"
                     /locus_tag="ACHY3M_003865"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFS4318689.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLGLESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5213..6469)
                     /gene="nupG"
                     /locus_tag="ACHY3M_003866"
     CDS             complement(5213..6469)
                     /gene="nupG"
                     /locus_tag="ACHY3M_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFS4318690.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6671..7750)
                     /gene="mltC"
                     /locus_tag="ACHY3M_003867"
     CDS             complement(6671..7750)
                     /gene="mltC"
                     /locus_tag="ACHY3M_003867"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFS4318691.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7815..8090)
                     /gene="yggX"
                     /locus_tag="ACHY3M_003868"
     CDS             complement(7815..8090)
                     /gene="yggX"
                     /locus_tag="ACHY3M_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFS4318692.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8118..9170)
                     /gene="mutY"
                     /locus_tag="ACHY3M_003869"
     CDS             complement(8118..9170)
                     /gene="mutY"
                     /locus_tag="ACHY3M_003869"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4318693.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9331..10050
                     /gene="trmB"
                     /locus_tag="ACHY3M_003870"
     CDS             9331..10050
                     /gene="trmB"
                     /locus_tag="ACHY3M_003870"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4318694.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10050..10376
                     /gene="yggL"
                     /locus_tag="ACHY3M_003871"
     CDS             10050..10376
                     /gene="yggL"
                     /locus_tag="ACHY3M_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFS4318695.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10560..11279
                     /gene="yggN"
                     /locus_tag="ACHY3M_003872"
     CDS             10560..11279
                     /gene="yggN"
                     /locus_tag="ACHY3M_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFS4318696.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11455..12501
                     /gene="ansB"
                     /locus_tag="ACHY3M_003873"
     CDS             11455..12501
                     /gene="ansB"
                     /locus_tag="ACHY3M_003873"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFS4318697.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12618..13625
                     /gene="yggM"
                     /locus_tag="ACHY3M_003874"
     CDS             12618..13625
                     /gene="yggM"
                     /locus_tag="ACHY3M_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFS4318698.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLAMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13681..14982)
                     /locus_tag="ACHY3M_003875"
     CDS             complement(13681..14982)
                     /locus_tag="ACHY3M_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4318699.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(14982..15485)
                     /locus_tag="ACHY3M_003876"
     CDS             complement(14982..15485)
                     /locus_tag="ACHY3M_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4318700.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(15530..16516)
                     /locus_tag="ACHY3M_003877"
     CDS             complement(15530..16516)
                     /locus_tag="ACHY3M_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4318701.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4400 a         3979 c         4214 g         4108 t
ORIGIN      
        1 catggtggtg agcggggagc gctactgtac agcctgatcg ggatgtgcaa actgaatgac
       61 gtggatccag aaagctacct tcgccatgtg cttggcgtca tagcagactg gccggtcaac
      121 cgggtcagcg aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc
      181 aatacggccc tcgctgtacg cttacgaagt taataccgtt ttgtctgttt ttttaacaaa
      241 gaaaataatc ctggagtagg ttcccgggca tttgcccggg atgattactg ttttaatgga
      301 ttattaatct ttgcatattc aaatggactg ataaattttt ctctgtttgc atccagaaaa
      361 tcggcccacc attgtagcat caagcggcgt tcttccagat gttctgcttt atgaatgtaa
      421 gcagcacgta caccgttacg ttcctgatgg ctcatctgac gttccacagc atcgcgggac
      481 cacaaacctg attcaatcaa tgcactgcac gccatcgccc ggaatccatg gccacagact
      541 tcctgggttg tgtcataccc catcaccctc agggctttat ttacagtatt ttcactgatt
      601 ggtttacgcg ggtcatgtgc acccgtaaat atcagaccat tttcacctgc ccatgtctga
      661 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
      721 ttggagcccc ggtgtgagaa ttttacgcct ggcagaggtt cgcgttcggc tggaatagtc
      781 cacatggcat tgtcaatatc aatttcagac cagcgggcgt atctgagttc actggaacga
      841 ataaaaacca gtaaattcag cataactgcc agtcgggtga caggctggcc tttataaccg
      901 tcaatacgcg acagaagttc aggcaggcgt gaaagatcca gagcagggcg atgctggcgt
      961 tttaccgtgg tcaatgcacc agccatatcc agagccgggt tatatctgat gatgccagac
     1021 tgtacagcat aacgcattac agcagatatt ctttgctgca gacgactggc gatttcataa
     1081 atttgtttag cttctgcagc acgaacaggg ataagcaggt ccggggtatt gagtgttgtg
     1141 atatcccggt tgccgataaa ggggaaaacg tgtgtttcga ggcttttgag taccttatcg
     1201 ctgtgtgatt gtgcccattg tttattactg gcatgccagc gtcgtgcaat atgttcaaaa
     1261 gagagttgta aatcagggac tactttttta gctcttttct gttcgcaagg gtcaatcccc
     1321 tctgaaatca gttttcgggc ccgttttgta cgagcttcgg agagagaaac ttctggatac
     1381 acaccaagtg ccagagtctt ctccttaccc agaatacgat aacggagacg ccagtaacgg
     1441 gaaccattag gatgaaccag caggaacatg ccagccccgt cagtgagttt ataagccttg
     1501 tcagagggct ttgcagcccg gatttttgcg tcagtcagtg ccatagtaca gccttctgaa
     1561 tgcgttaaag agggtacaaa ttctgtcgaa ctggggtata cccgcaatta tacccacaca
     1621 atggggttga tgtgggggga atctggttga gttcggtaga attgatttgt aggtagaacg
     1681 cttaaatcat ggtgttctga aacgaaaaac ggacctccgt ggaggtccgt tgatatgaat
     1741 ttggtgcccg gactcggaat cgaaccaagg acacggggat tttcaatccc ctgctctacc
     1801 gactgagcta tccgggcaac ggggcgcatt aaacctgatt cgcctcgtct cgtcaatcaa
     1861 atttcttcaa tttactgcag actgcacaat ctatcatcac tttgccgcta ttgcacgcat
     1921 tgtcaggcaa accaggcggt ccaggcggcg gaaagcggca ttgccagaag gagtgctgga
     1981 agcatgttga ccaccgggaa cattttaatg ccacagatgc gtaatccggt tgccagcagc
     2041 aataaaccgc ctacggcgct gaagtctgcc atcatcgacg gtgtggtcag cggtaatatc
     2101 agcgcggcat cccacgccag cgttaactgg atgatcagta atgggatact aattaccgac
     2161 accgcaatac ccagcgagca ggcgaagatc atcgccgtaa agaaatcaag aaatgacttg
     2221 gcgattaaaa tactcggatc gccggtcatc ccttcgttca tcgccccgaa gatcccggtg
     2281 ccgctggcgc aaaacaggac aataatcgcg acataattct gaataaaaga ttcatgcgct
     2341 ggcttcttac gtgagtgacg aaacagattt tgtgctttgg cgactgctgt attgacacct
     2401 ttctccagca aacaaatttc gccaattaga gcgccaagta gcgtagctaa aaccatcgct
     2461 ggaaggttgg cgcatttcac aaccagtaaa ataccaatcc ccagcgatgc cagaccaaaa
     2521 atagatgtca tggagacgcg gatacgttcc ggtaagcgtt ggctgagcag tgcgccgaga
     2581 acgccaccca gtaagactgc actagcgttg ataaaagggc cgatgaccac aagagttcct
     2641 gttagctgta ttgtttgatt ttcttattat gaactttttg ccttgttgat ggcttgtctg
     2701 gtcgcaaagt cctggcttgc acgctttagc gaaaggtgcc atgattgcgc gaattttctc
     2761 ctctctgtac ggagtttgcc cgatgcacgc catctcctta cattctctcg cttatcgccg
     2821 tttcgcgcga aacgtttccc tttctatgtt actgctcatg cggtgaagtt aacgcacgct
     2881 cactgcagga caacagtaaa atcagagcgt ttctgctttt actgatgtct ggcggtcgga
     2941 gctggtgacc agtttgaccc atatctcatg gggcagggtt ttccaccttg tccggtattc
     3001 ttacttcccc gaaacgggtt tgcgcttatg aaatcaatga atattgccgc cagtagtgaa
     3061 ctggtatccc gactttcttc tcatcgtcgc gtggtggcgt tgggagatac tgattttacg
     3121 gacgtcgcgg cagtcgtcat taccgctgcg gatagtcgca gtggcattct tgcgttgctt
     3181 aagcgcaccg gttttcatct accggtgttt ttgtattccg aacatgctgt tgaattacct
     3241 gcgggcgtta cggcggtaat caacggcaac gagcagcagt ggctggggct ggaatccgca
     3301 gcctgtcagt atgaagagaa tttgctgcca ccgttttatg acacgctgac gcagtacgtt
     3361 gagatgggca acagcacctt tgcttgccct ggacatcaac atggtgcgtt ttttaaaaag
     3421 catcctgccg gacgccattt ttacgatttc tttggtgaga acgtctttcg cgccgatatg
     3481 tgtaacgctg acgtaaaatt gggcgatctg cttattcatg aaggatcggc gaaagatgcg
     3541 cagaaattcg cagccaaagt ctttcatgcc gataaaacct attttgtgct gaacggcaca
     3601 tcggcagcga ataaagtggt gacgaatgcg ctgttaacgc gtggcgatct ggtgctcttc
     3661 gaccgtaaca accataagtc gaatcatcac ggcgcgctga ttcaggcggg ggcgacgccg
     3721 gtctatctgg aagcttcacg caacccgttt ggtttcattg gcggtattga tgcgcactgt
     3781 tttaatgaag agtatctgcg ccagcaaatt cgcgacgttg cgccagaaaa agccgagctg
     3841 ccgcgcccgt ttcgcctggc gattattcag ctgggaacct atgacggaac tgtctataac
     3901 gcccgtcagg tgatcgatac cgttgggcat ctgtgtgatt acattctgtt tgattccgcg
     3961 tgggtcggtt acgaacaatt tatcccgatg atggcggata gctcgccgct gctgttagaa
     4021 cttaacgaaa acgatccggg gatttttgtg acccagtcgg tgcacaaaca gcaggctgga
     4081 ttctcacaga cgtcgcagat ccataaaaaa gataaccata tccgcggaca ggcgcgtttt
     4141 tgcccgcata agcggttgaa taacgccttt atgctccatg cttctaccag ccctttctat
     4201 ccgctgtttg ctgcactgga tgttaacgcc aaaattcatg aaggggagag tgggcgtcgg
     4261 ctgtgggctg agtgcgttga gttgggtatt gagtcgcgca aggctattct tgcgcgctgt
     4321 aagctgttcc gcccgtttat cccgcccgtt gttgatggca aattgtggca ggattatccg
     4381 acatctgtgt tagccagcga ccgccgtttt ttcagttttg agccgggggc gaagtggcac
     4441 ggctttgaag gatatgccgc ggatcagtat tttgttgatc cgtgcaagct gttactcact
     4501 acaccaggta tcaatgccga aactggcgaa tatagcgact ttggcgttcc ggcgacgatt
     4561 ctggcgcact atctgcgtga gaacggcatt gtgccggaga agtgcgatct caactccatt
     4621 ctgttcttat taactccggc ggaaagccac gagaagctgg cacaactggt ggcgatgctg
     4681 gcgcaatttg aacagcatat tgaggatgac tcgccgctgg ctgaggtgtt gccgagcgtt
     4741 tataacaaat atccggtgcg ctatcgcgac tacaccctgc gccagttgtg tcaggagatg
     4801 cacgatctgt atgtcagttt cgacgtcaaa gacctacaaa aagcgatgtt ccgccagcag
     4861 agtttcccgt cagtggtgat gaacccccag gatgcgcata gcgcttatat tcgcggtgaa
     4921 gtggagttgg tgcggattcg tgatgccgaa gggcgaattg cggcagaagg ggcgttgcct
     4981 tatccacctg gcgtgctttg cgtggtaccc ggggaagtct ggggtggggc ggttcaacgt
     5041 tatttccttg cactggaaga aggggtgaat ttgttgccgg gattttcgcc ggagctgcaa
     5101 ggtgtttata gcgaaaccga tgcggatggc atgaaacggt tgtacggtta tgtgttgaag
     5161 taagaataaa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt aattagtggc
     5221 taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc gccatgaacg
     5281 cgaaggccag aaccacggag taaccagcga aaatcaacca tacggtctgc cagtcggtaa
     5341 tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg aggatacagc
     5401 cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt gccgggctaa
     5461 cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa
     5521 cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg taagcaaaca
     5581 gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta ataccgtagc
     5641 ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc gaaatcgaca
     5701 tgatgattga cgcatgctgc acaataaagc tgctggcaaa catcggatct ttgtcgaagc
     5761 tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc agcagcattg
     5821 agaagataaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca tcgaggccca
     5881 gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc ggcagagtca
     5941 gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc atgtggctta
     6001 attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg gtgccccaga
     6061 tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg cgatagtaag
     6121 agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc aatatcacaa
     6181 ggaacatcgc ctccggtgtc gtgacctgtg ccgccatgaa cagcgtgata gcgccaatgg
     6241 tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca atccccagca
     6301 gcgcaggcat aaagaccgcg gcgataccca gtgagctata aactgcgcca atagaagcac
     6361 cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa cttccccaca
     6421 gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg ttaatttcct
     6481 cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag atgatgggat
     6541 tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca aacattccca
     6601 atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc gtcaggggcg
     6661 gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta taaagataac
     6721 ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg cccggcgtca
     6781 tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc agcacgctgc
     6841 ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg ttatcaattc
     6901 cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca atattgctgg
     6961 caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag cggaacacat
     7021 ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa cggctgaccg
     7081 catatgggtt aaaggaagat tcggtctgca taatcgccag aatcagcgac tcatcaacgc
     7141 catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt ttatcaaggt
     7201 ggttcggcac catgttaatg gtaacgctgt agatgatacg cagcccgttg ctgcggctct
     7261 tcagacggtt tttcagcaga taatccgcga agttgctcgc gcgaccttcc cagcgaatcg
     7321 gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa atcgtaatat
     7381 catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc aacgttttga
     7441 taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg atagtaatcg
     7501 taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat ttcacgtagt
     7561 ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata ttgtgggcaa
     7621 attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg ttataggtat
     7681 cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc agcgcgagat
     7741 attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc cggaggcgtg
     7801 ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg cacctcttta
     7861 ccctcgaaca ggaagttgac catctcctgc tccagcagct tgcggtgctc ggcattcatc
     7921 atgttgagtt tcttttcatt aatcagcatg gtttgtttgt gctgccactg cgcccaggct
     7981 tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag ctgaaaatcc
     8041 tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat aaatcatcct
     8101 ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa caaacgctcc
     8161 acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag cgcattgcct
     8221 tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat atctaagtgg
     8281 aaatggctga aggtatgccg aaacgcggtc agctgcgtca ggttatcggc agcaatctgc
     8341 cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa acagtataaa
     8401 ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc gtgctgtaat
     8461 agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc cggataaagc
     8521 gcccagctat tgttggcggc ggcaatacat ccgttttgta gcggacagag cgaacatttc
     8581 ggtttcgagc gcgtacaaat catcgcaccc aaatccatca tcgcctgatt aaaccgttcc
     8641 acgccaaccg cgggcgtcac ctgctcgctc aaactccata atttattctc gacctctttt
     8701 ttccctggcc agccgcttac agcatagcag cgcgccagca cccgtttgac gttaccgtcg
     8761 agaatcggaa agtgcttacc cagagaaagc gagagaatcg cacctgcggt ggaacgcccg
     8821 acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc gtgtaaggtc
     8881 gccacttgtt gtgccgcttt atgcaggttg cgcgcgcggg cgtaatagcc aagcccggtc
     8941 cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt cgggaagcgc
     9001 gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg caacatcact
     9061 tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt tttccgcccg
     9121 tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat ggtcaccgaa
     9181 ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa ccggaacttt
     9241 ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa tgcccgtttt
     9301 cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc accggaattt
     9361 gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca ggggcgactg
     9421 accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt tgagttcagc
     9481 gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac gcttgagatt
     9541 ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga gcaggacttc
     9601 ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc gcatgaagaa
     9661 ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca taaaatgatt
     9721 cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca caaagcgcgc
     9781 cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag caaactgcag
     9841 ctggggggcg tattccatat ggcgaccgac tgggaacctt atgcggaaca tatgcttgaa
     9901 gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta cgtaccgcgt
     9961 ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg tcacggagta
    10021 tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt cgtctgcgta
    10081 aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg cgattcccgg
    10141 aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac gaggttatcg
    10201 aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt ctgatctgca
    10261 tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag tggctggaag
    10321 agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg gactaagaaa
    10381 gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca ggcctggaaa
    10441 gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg gaaaacgcgt
    10501 actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga gggaagacta
    10561 tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc gctcacgccg
    10621 actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa accgtgcagg
    10681 tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg tataacggta
    10741 agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag gctgaactac
    10801 gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa gcccgtattg
    10861 cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc agccgtctga
    10921 ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg cgcagcgatg
    10981 gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag caattagtga
    11041 atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg aaagcggtgc
    11101 tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg ctgcaatcct
    11161 caatccaaac cgagtggaaa aagcaggaaa aagatttcca gcagtttggc aaagatgttt
    11221 gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat ttaaaataat
    11281 cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt tttgttacct
    11341 gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta acgtcaaatt
    11401 tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa tgaaatggag
    11461 tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc agcattggca
    11521 ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg tgactccgca
    11581 accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa tgcggtgccg
    11641 caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg ctcccaggac
    11701 atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg cgataaaacc
    11761 gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta cttcctcgac
    11821 ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc gtccacgtcc
    11881 atgagcgcag acggtccatt caacctgtat aacgcggtag tggccgcagc tgataaagcc
    11941 tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg tcgcgatgtc
    12001 accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg tcctctggga
    12061 tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca caccagcgat
    12121 acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt ttataactac
    12181 gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga tggcatcgtt
    12241 agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct ggcaaccgcc
    12301 gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc taccactcag
    12361 gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct gaacccgcaa
    12421 aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca gcagatccag
    12481 cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct ttttcacttc
    12541 agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa taaattattc
    12601 cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg cttatgttga
    12661 tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta aaagatcaat
    12721 tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa aatcttgatg
    12781 ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt gacgatctct
    12841 atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg acgaaagata
    12901 aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct ggctggtcgg
    12961 tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc gacgatatca
    13021 aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc tttagtcagc
    13081 ttgaatctgc gttgaatacc cagaagaact cgattcctgc gctggaaaag gaagtgaaag
    13141 cgcttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg ggaaaagatg
    13201 caaacggtaa gcaaatgacc cgcgaagaag cctttaaaaa aattcaccaa cagcgtgatg
    13261 agtttaataa acagaacgat agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc
    13321 aaccggcgat tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg ccgattcagc
    13381 agaaacgaga tttcgatatc aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac
    13441 tcagccgtaa attgcaggat gactgggtaa cgcttgaaaa agggcaatat ccgttagcga
    13501 tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt gatgaaaatt gacgatatca
    13561 accaagctaa tgaacgctgg aaaaaagata ccgaacaact gcggcgcaat ggggtgatta
    13621 agtaagtttg atatagtgcc tttgccggat aatataaaaa gttatccggc aaattatttt
    13681 ttacattagc cctagtagct gtggaatgaa caaggatatt tgcggaatgt aagtgatcat
    13741 catcaacgct actagcaatg cagcataaaa tggcagcaat ggtttgatta aatgttgaat
    13801 cttcacaccc gagatagaac aaccaacaaa tagcgcacta cccaccggcg gtgttaacag
    13861 cccaatacaa aggttggcca ccatcataat accgaaatgt actgggtcca ttcccagctc
    13921 ttgtgcaatc ggcaagaaga tcggtgtaaa gattagtaca gccggtgtca tgtccataaa
    13981 gatgccgaca attaataaga cgatattaat cagcagcaat atgatcagtg ggttatcaga
    14041 aatccccatc aatgcgtcgc tgatcatata aggaatatca gcattagtca ttgcccagga
    14101 catgcccacg gaaaaaccga tcaacagtaa tacaatcgaa gtggtaacaa ccgactccag
    14161 aatcagtttg ggtaaatggc gccattttac ttcccggtaa accagcaccg agagaataaa
    14221 tgtgtagaca acggcaattg ctgacgcttc agttgcggta aaaatgccgc ccagaatgcc
    14281 gcccatcaca atgaacacca gaagcaaact tggtaaggca tccagaaacg ctttacaggc
    14341 ctgagcaaag gttgctcttt ctgaaagcgg gtagccacgg cgtttagcaa taatgccgca
    14401 gactatcatt accgcaagac ccattaagat ccctggcaaa tatcccgcca taaatagcga
    14461 tgctacagaa acgccgccag ctgtcaggga aaacacaatt aacacgtttg agggcggtat
    14521 cagtaatcca gtaatacaag atgagacgtt tacagcggta gaaaatgccg gatcataacc
    14581 ttgtttggtc tgaatgggat tcagtgtgcc accaacggca gcagctgccg caacggctga
    14641 accagaaatg gaaccaaaca tcatattcgc caggacgttc acatgaccaa gtgaaccagg
    14701 aacccgacca accattacct gggcgaggtt aatcaaacgt atcgcaatac cgccgctgtt
    14761 catcagcgtt ccggcaaaga tgaaaaaggg gattgccaat agggcaaagt tatccagtcc
    14821 attagccagt cgctgcgaaa cagtgattgc cgcgatatcc cagggaaaca tcaaaagcat
    14881 tgaagctaca gtggcaatac cgattgagaa cgaaatgggc acaccaataa agaccaacac
    14941 aaaaaaacag ccaaacagtg taatcgcgat ataactatcc attaactggc ctcctgcgca
    15001 tgttgtttca gttgttgaat gctttgatta ataaaacaca aggcataaaa catcatcacc
    15061 aatccactta atggcagaat gatatatacg tatcccatcg gtatttgcat cgcggcagat
    15121 acctgtgatg ttgccagcgt tttatcaatt aatttcaggc caccagtcac aatgaccgaa
    15181 ccggcaaaac ctaaaataag aacgttaatg actatactaa gaagtaattg cttgcgctta
    15241 ttcagggcga gagcaaataa atcgatagag agatgtctct gcgcgccgac ggtatatgca
    15301 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
    15361 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
    15421 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
    15481 atcattgtaa gcatcctgga aaatcggggc cagagactgg cccggaatat tattgagcag
    15541 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
    15601 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
    15661 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
    15721 cggatttctt cgctgctgtt tcgaggattt gttgctgttc tggggttaat ttattccatg
    15781 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
    15841 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
    15901 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
    15961 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
    16021 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
    16081 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
    16141 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
    16201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagag
    16261 cgccattttg caacaactcc atagtttctc gcgcattacc catctggctg ctgggataaa
    16321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
    16381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
    16441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
    16501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
    16561 tgtgcttatc agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
    16621 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
    16681 gtttataatc aataaatgac g
//
LOCUS       DAZUFB010000106        16607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZUFB010000106 DAZUFB010000000
VERSION     DAZUFB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.106"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..634)
                     /gene="rsmG"
                     /locus_tag="ACHY3M_003878"
     CDS             complement(11..634)
                     /gene="rsmG"
                     /locus_tag="ACHY3M_003878"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4318702.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(698..2587)
                     /gene="mnmG"
                     /locus_tag="ACHY3M_003879"
     CDS             complement(698..2587)
                     /gene="mnmG"
                     /locus_tag="ACHY3M_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4318703.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLERSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2965..3408)
                     /gene="mioC"
                     /locus_tag="ACHY3M_003880"
     CDS             complement(2965..3408)
                     /gene="mioC"
                     /locus_tag="ACHY3M_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFS4318704.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3498..3956)
                     /gene="asnC"
                     /locus_tag="ACHY3M_003881"
     CDS             complement(3498..3956)
                     /gene="asnC"
                     /locus_tag="ACHY3M_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFS4318705.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4108..5100
                     /gene="asnA"
                     /locus_tag="ACHY3M_003882"
     CDS             4108..5100
                     /gene="asnA"
                     /locus_tag="ACHY3M_003882"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFS4318706.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5105..6556)
                     /gene="viaA"
                     /locus_tag="ACHY3M_003883"
     CDS             complement(5105..6556)
                     /gene="viaA"
                     /locus_tag="ACHY3M_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFS4318707.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6550..8046)
                     /gene="ravA"
                     /locus_tag="ACHY3M_003884"
     CDS             complement(6550..8046)
                     /gene="ravA"
                     /locus_tag="ACHY3M_003884"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFS4318708.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8269..10137
                     /gene="kup"
                     /locus_tag="ACHY3M_003885"
     CDS             8269..10137
                     /gene="kup"
                     /locus_tag="ACHY3M_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFS4318709.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10304..10723
                     /gene="rbsD"
                     /locus_tag="ACHY3M_003886"
     CDS             10304..10723
                     /gene="rbsD"
                     /locus_tag="ACHY3M_003886"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4318710.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10731..12236
                     /gene="rbsA"
                     /locus_tag="ACHY3M_003887"
     CDS             10731..12236
                     /gene="rbsA"
                     /locus_tag="ACHY3M_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFS4318711.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12241..13206
                     /gene="rbsC"
                     /locus_tag="ACHY3M_003888"
     CDS             12241..13206
                     /gene="rbsC"
                     /locus_tag="ACHY3M_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFS4318712.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13231..14121
                     /gene="rbsB"
                     /locus_tag="ACHY3M_003889"
     CDS             13231..14121
                     /gene="rbsB"
                     /locus_tag="ACHY3M_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFS4318713.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14247..15176
                     /gene="rbsK"
                     /locus_tag="ACHY3M_003890"
     CDS             14247..15176
                     /gene="rbsK"
                     /locus_tag="ACHY3M_003890"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4318714.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15180..16172
                     /gene="rbsR"
                     /locus_tag="ACHY3M_003891"
     CDS             15180..16172
                     /gene="rbsR"
                     /locus_tag="ACHY3M_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFS4318715.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16138..>16607)
                     /locus_tag="ACHY3M_003892"
     CDS             complement(16138..>16607)
                     /locus_tag="ACHY3M_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4318716.1"
                     /translation="VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFIL
                     GMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGMEG
                     TTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE"
BASE COUNT         3943 a         4114 c         4362 g         4188 t
ORIGIN      
        1 ttgataaaaa ttaaatttta tttgctttaa tcaccaccag atgacgttcg ccatccaggg
       61 ctggaacctg aagtttaacc actgattcga cctgatattc ttcgggcaac aaagcgattt
      121 catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca ggaagatggt
      181 ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg ccatcaaatg
      241 gcggctctga aggaaactct tctaccctgc tctgtactgg ttcaatattc tccagtttaa
      301 gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc aacagagtga
      361 aatgggcttc aggacgcacg atagagagtg gaatgcctgg cagtcctggt ccggtgccga
      421 catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg agaatatggc
      481 gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg ttccatttat
      541 gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc gaaatacctg
      601 cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct tgatgcgttg
      661 cctggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga cgcagcatac
      721 cctgtttttt cagccacacc agcagaatgg agatggccgc aggcgtgacg ccagaaatac
      781 gcgaagcctg gccgatagag gctggtttgt gatcgttaag tttggcgatc acttcgttag
      841 aaagaccgga tacctggcgg taatccagtg tcgcgggtag cagggtgttc tcgttacgca
      901 gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta acctgaatct
      961 caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc agcgtggtta
     1021 atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct tcacgggaaa
     1081 gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg gttacccagg
     1141 tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta aagcgcgccc
     1201 aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc agatccgcat
     1261 tatcttcgcg tagcatcaga cgatattctg cgcgcgaagt aaacatacga tacggttctt
     1321 tggttcctaa agtgcacagg tcatcaacta gtacgccgag atacgcctga gaacgtgccg
     1381 gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccggcc agcaaacctt
     1441 gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag aacagcccct
     1501 ggataaactt gctctccagc gtcggtttca ggtcgcgagg atcgaagaag tcatactcaa
     1561 tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatagag cggacgattt
     1621 gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt tcattagagg
     1681 tcagtccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag cgcataactt
     1741 tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct gcatacattg
     1801 ggctacgctc gaggttactg cggatcacat catgggtttt ctcgttggta tgagtgatat
     1861 aacacggcac ctgctgggga tgctgggacg cattgcccat aaacgagaat accggcattg
     1921 ggttatcgcc atgctgttgc gccagcacac taaagtcgat agttcgagca tcaatacgcg
     1981 gtggtgtccc ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa cggcgagaaa
     2041 gcggaatgga cggcggatca ccagcacggc caccgctgta attatccaga ccgatatgaa
     2101 ttttaccgtc gaggaacgtc ccaacggtga gcacgacggc tttggcacgg aacttcagtc
     2161 ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct tcaaccgcct
     2221 gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc tgacggtaga
     2281 gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg tcctttgctt gcgtttagta
     2341 tcctaaactg gatacccgct tgatcgatcg ctttcgccat cagaccgccg agtgcatcca
     2401 cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag ctcatctgcc
     2461 ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc gccgcggcca
     2521 tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa ggatccggat
     2581 aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc cgggccgtgg
     2641 attctactca actttgtcgg cttgagaaag acctgggatc ctgggtatta aaaagaagat
     2701 ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact ccctgtggat
     2761 aacaaggatc cggcttttaa gatcaacaac ctggaaagga tcattaactg tgaatgatcg
     2821 gtgatcctgg accgtataag ctgggatcag aatgaggggt tatacacaac tcaaaaactg
     2881 aacaaccgtt gttccttgga taactaccgg ttgatccaag cttcctgaca gagttatcca
     2941 cagtagatcg cacgatctgt atacttattt gagtaaatta atccacgatc ccagccattc
     3001 ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg atcttcagtg tttcgcctgt
     3061 ctgttttgca ccggaatttt tgagttctgc ctcgagttta tcgatagccc cacaaaaggt
     3121 gtcatattca cgactaccaa taccgattgc gccaaagcgg accgcagaaa gatcgggctt
     3181 ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc ggaatatctc cggcaccgtg
     3241 ggtggagctg ataatcagcc agatccccga ggcagataaa tcttccaaca gcggaccgtg
     3301 cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca gccaggtgtt ctgctacata
     3361 ttcggcaccg ccgagggtgc tgccgctgat aagagtgata tctgccataa accgccacct
     3421 ttattaagag tggcgtattg tacgctgtga acgtgttggg atctacctgt ggaaaagtat
     3481 gggattaaaa aagccgatta tggcttgatg gtacgcatga tcgggttttg caggacgatc
     3541 aatgtctcgg tggactgaat ttcatcaatt gtttggatct tgttgataag tacatgctgg
     3601 agagcgtcga tcgaacggca catcactttt ataaagatgc tgtagtggcc ggttgtgtaa
     3661 taggcttcag tgacttcatc aaggctttcc agctttgcca gcgcggaagg gtagtctttg
     3721 gcgctcttta atataatgcc gataaagcag cctacgtcat aaccgagctg cttcgggctg
     3781 acatcaatac gcgccccggt aatgatcccc gcctgcttca ttttctctac tcgaacgtga
     3841 atcgtccccg gactgacgcc aaattgtttc gccagttcgg cgtaagcggt gcgcgcattg
     3901 cccattaatg cttccaggat gccacggtcc agattgtcga tcagataatt ttccatagga
     3961 ttttcttatg cagattgatg attcattcta ttttagcctt cttttttaat gaatcaaaag
     4021 tgtgttaggc tttttattga atgattattg catgtgtgtc ggtttttgtt gcttaatcat
     4081 aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt acattgccaa acaacgccaa
     4141 attagcttcg tgaaatctca cttttctcgc caactggaag aacgtctggg gctgatcgaa
     4201 gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc aggataactt gtcgggctgt
     4261 gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg cccagttcga agtggttcat
     4321 tcactggcga agtggaaacg tcagacctta gggcaacacg acttcagcgc gggcgaaggg
     4381 ctgtacacgc acatgaaagc ccttcgcccc gatgaagagc gtctttctcc gttgcactcg
     4441 gtctatgttg accagtggga ctgggaacgc gtaatgggcg acggtgagcg tcaattctcg
     4501 actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga agctgcagtt
     4561 agcgaagagt ttggtttggc accgttcctg ccggatcaga tccacttcgt acacagccag
     4621 gagttactgt ctcgttatcc ggatctcgat gccaaagggc gtgagcgggc gattgcgaaa
     4681 gatcttggcg cggtattcct cgtcgggatt ggcggcaagc tgagtgatgg tcatcgtcac
     4741 gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg ccatgcgggt
     4801 ctgaacggcg atattctggt atggaaccct gtactggaag atgcgtttga gctttcttcc
     4861 atggggatcc gcgtggatgc cgacacgttg aagcatcagt tggcgctgac cggtgatgaa
     4921 gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg gtgaaatgcc gcagaccatc
     4981 ggcggcggta tcggccagtc tcgtttgacc atgctgctgc tgcaactgcc gcatatcggc
     5041 caggttcagt gtggagtatg gccagctgct gttcgcgaga gcgtcccttc tctgctgtaa
     5101 taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat cccggtatca aagcgccaga
     5161 tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc cgacatcgcc acggcatgaa
     5221 agcgatgctg atgtacccgc tgcagctctt tcactttact cgtcacgtcg tcaggcaacc
     5281 gctgagcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc ctgctttgca
     5341 agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga aactgctggc
     5401 ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa cggacgatct
     5461 cggtggaaaa tagcataata tagcagcgcc ggttttcagc gagagcaatg cgcatcaagg
     5521 ccaggcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg gaagtatcca
     5581 cacagacaat aaacggcccg cgcggctgtt catcgtaatc tttatgtacc accggacgtt
     5641 cgatcacttt ttcacgccac gactcaccgt gcaggcgata ggtgagcaac tgtttttcca
     5701 ccagccgacg gtaaaactca tactccagtt ccgttatccc tagtgtcgcc agttctggcg
     5761 gcagaagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca ggaaccgtcg
     5821 ccggttcgcg caccatggtg cggaaggttt ccatctgcgc atcgttgcgc ggtattgatt
     5881 tggcttcccg agaacgcccc agctgctctg ccaggcgttt cagttccggc tgttcgttaa
     5941 gaaattcacc gtatttcaca atcaactgat agtcgccacg tttaagctga ccggcgctca
     6001 tatcccacag acgaccagct gcagtattgt tatctgcgag aatcggttca agttgtccgc
     6061 tcagcgtcat gcgttcctga acttcactca acagttgttc gcgttcttct tctaatagct
     6121 gttgatttaa cgtcgttgct tgcacgatca gacttaaacg ccaacgctgg agaaaaagcg
     6181 tgtgtaacgc tgaagtgatc gtgctgttag catcaaccaa ctgtcgggct tgttctgccc
     6241 atggagaatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca ataaactgtg
     6301 gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt tccggcggga
     6361 ctcgggcatc tttcagccga ctgcgcagcg cttcacgcca gcggggaaca tcatcagtga
     6421 ttgccgcctt caggcgtggg aatttttcaa agaagaccgc cagctgcggt gaggccagca
     6481 gtgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc acattaagcg
     6541 tatccagcgt tagcattgtt gtgcctggcg aatctgtgca ccgacatcct gtaggctggc
     6601 ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt cgctgaataa
     6661 agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt tgttgtttta tttcaccagg
     6721 cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac ttacatcgcg
     6781 aacgacaaga tgttgggcgc tatcaacttc cagattcagt ttctgggcaa agccgatacc
     6841 gttcagttta ccgcgaattt caccaccttt gctcagccac tgttccagcg cgctacgctc
     6901 aaaggagata tgaaccactt ccatatcatg cagttttaac ggtttttgca gcagtagagt
     6961 cagagtggaa gcagtaacgt taacaggaag ttgatactgc tgacgacggc tgaaaatgcc
     7021 gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgga gttgcaggtg
     7081 acgttgcaca atcgcgccaa ggcgagtcaa catcccttgc tgttgccagg cgtgaccggt
     7141 catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat accacaggca
     7201 atctttcagc aaaatgagat ctaccggggc aacagcactg cgaccgctaa aaaaggcgct
     7261 ggcctgcaat aatcggatcg cttttttcca gcgacgatcc gagacataag gcgcatccgg
     7321 taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat cgggcagcgt
     7381 aatttcacca atctctttct gccagcgttc atattcttca tctgtgacct gcaaggcatc
     7441 aggaaccgga ttgtcgtttt catcctgttg actggtcagc atggagcgga aattcgcttt
     7501 atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg cttccagact
     7561 gctgtctgct tccggcagct cgttggaggc cgccaccagc agacgcatcg ggattttttc
     7621 tacgtgtgca ccgttgcgga actggcgctc gttaatggcg gtgagcaagg tattaagaat
     7681 tgccgggccc gctttccaga tctcatccag aaagacaatt tctgcttccg gcaggtaacc
     7741 gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa ggggaccaaa
     7801 aacttcttcc ggcgtggaga agcgggtcat cagatattca aacgcgcggg cattctgaaa
     7861 ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggcgggc caaggaggaa
     7921 cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc tacgttcata
     7981 aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata aatgagggtg
     8041 agccataatg aagtggcgtc ctttcgtcaa aagttctgcg taaattgcga gtatagacgt
     8101 ttcttgctgg tggctaaaat agtctcaaag ggggggtatt tttctttgag ccaggttaat
     8161 gtggccgcat ttaggagtac gattttgccg ttaatcgtgc atactgtgcg cttttttgtg
     8221 ggccaaggga ctaagcacac atttcatatt tcaacgaaag actagtctat gagcactgat
     8281 aataagcaat cattgcccgc gattaccctc gcggcgattg gagttgtcta cggcgatatt
     8341 ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg gccagtttgg ttttggcgtt
     8401 gaacgcgatg ccgtgtttgg ctttttatcg ctgatcttct ggctgctaat ctttgtggtt
     8461 tccattaaat atctcacctt cgtgatgcgg gcagataacg ccggtgaggg ggggatcctg
     8521 acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa ccacatcaat gctggtgatt
     8581 atggggctaa tcggcggcag ctttttctat ggtgaagtcg tcataacacc cgctatttcg
     8641 gtgatgtcag ccattgaagg tctggaaatc gtcgccccgc agctggatac ctggatagtt
     8701 cccctctcaa ttatcgttct cactttatta tttatgattc aaaaacatgg cacggctatg
     8761 gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt tgattctggc aggattgggg
     8821 ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc tgaatccaat gtgggcggtg
     8881 catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt ggtgttgtcg
     8941 attacggggg tcgaggcgct gtatgctgat atggggcact ttggtaagtt ccctattcgc
     9001 ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta attacttcgg tcagggagcg
     9061 ctgttgttaa agaacccgga agcgattaag aacccgttct tcctgttggc accggactgg
     9121 gcgctgatcc cgctgctgat catcgccgca ctggcgacgg taattgcctc gcaggcggtt
     9181 atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg
     9241 cgcattattc acacctccga aatggagtca gggcaaatct atattccgtt tgtgaactgg
     9301 atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag caacctggcg
     9361 gcggcgtacg ggattgcggt gaccggaacc atggtgctga cgtctattct ctcgactacc
     9421 gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct gattgctttc
     9481 ctttgtgtcg atattccatt gttcaccgct aacctcgata aactgctctc cggcggctgg
     9541 ttgccattga gcctcggtac tgtgatgttt atcgtgatga ccacctggaa gagcgagcgt
     9601 ttccgcttgc tgcggcggat gcatgaacat ggtaactctc tggaagcgat gattgcttcg
     9661 ctggagaaat caccgcctgt tcgcgtgccc gggaccgcgg tgtatatgtc gcgtgcaatc
     9721 aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt gcatgagcgg
     9781 gtgattctgt taactctgcg taccgaagac gcgccatatg tccataatgt ccgtcgggta
     9841 cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg ttggcgagaa
     9901 acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag ttgccggatg
     9961 atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat
    10021 ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg tgcaccagat
    10081 caatttgaaa ttccgccaaa cagggttatc gaactgggta ctcaggtcga aatctaacgc
    10141 cagacgcctc ctttctccat aagggggcgt ttttgttttc atggttaatc accatgtaaa
    10201 acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac tcagtcagga
    10261 ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt
    10321 tcttaattct gatatttcat cggtgatctc ccgtctggga cataccgata cgctggtggt
    10381 gtgtgatgct ggtttaccta tccccaaaag tacaacgcgt atcgatatgg cattaaccca
    10441 gggtgtacct tcttttatgc aggtgctggg cgtcgtcaca aatgaaatgc aggtcgaggc
    10501 tgccattatc gcggaagaga tcaaacaaca taatccgcaa ctccacgaaa cgttgctcac
    10561 tcaccttgag cagctgcaaa aacaccaggg aaataccatt gaaattcgtt acaccacgca
    10621 tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg gagaatgttc
    10681 tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc atggaagcat
    10741 tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc tcgggcgcag
    10801 cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaaacggc gcgggtaaat
    10861 ccaccatgat gaaagtgctt actggtatct atgctcgcga tgccggcacg cttttatggc
    10921 tggggaaaga aacgacattt accgggccga aatcttccca ggaagccggg attgggatta
    10981 tccatcagga actgaacctg atcccgcagt tgaccattgc cgaaaacatt ttcctcggtc
    11041 gtgagtttgt taatcgcttt ggcaaaattg actggaaaac catgtatgcc gaagcggata
    11101 aattgctggc taaacttaac ctgcgcttta aaagcgacaa gctggtgggc gatctttcca
    11161 tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag ctttgagtcg aaagtcatca
    11221 ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccctg ttccgcgtca
    11281 tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa
    11341 tcttcgagat ttgcgatgac gttaccgttt ttcgtgacgg gcaatttatt gctgagcgcg
    11401 aagtggcatc actgaccgaa gattcgctga ttgagatgat ggtgggccgc aagctggaag
    11461 atcaatatcc acacctggac aaagcgccgg gagatatccg cctgaaagtc gataatctct
    11521 gcggacctgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt cttggcgtct
    11581 ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt gctctacggc gcactaccgc
    11641 gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttca ccgcaggatg
    11701 gcctggcaaa cggcattgtg tatatctccg aagaccgtaa acgtgacggt ttagtgttgg
    11761 gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg ctacttcagc cgcgctggcg
    11821 gcagtttgaa gcatgccgat gaacagcagg ctgtgagtga tttcattcgt ctgtttaatg
    11881 tgaaaacacc gtcgatggaa caggcaattg gtctgctttc cggtggcaat cagcaaaaag
    11941 tggcgattgc ccgcggtctg atgacacgcc ccaaagtgtt gatccttgat gaacctaccc
    12001 gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact gattaaccag ttcaaagccg
    12061 atggcttgag catcattctg gtgtcatcgg agatgccaga agtattaggc atgagcgatc
    12121 gcatcatcgt catgcatgaa gggcatctca gcggggaatt tactcgtgag caggccaccc
    12181 aggaagtgtt aatggctgcc gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa
    12241 atgacaaccc agactgtctc tggtcgccgt tatttcacga aagcgtggct gatggagcag
    12301 aaatcgctta tcgctctgct ggtgctgatc gcgattgtct cgacgttaag cccgaacttt
    12361 ttcaccatca ataacttatt caatattctc cagcaaacct cggtgaacgc cattatggcg
    12421 gtcgggatga cgctggtgat cctgacgtcg ggcatcgact tgtctgtagg ttctctgttg
    12481 gcgctaaccg gcgcagttgc tgcatctatc gtcggcattg aagtcaatgc gctggtggct
    12541 gtcgctgccg ctctcgcgtt aggtgccgca attggtgcgg taaccggggt gattgtagcg
    12601 aaaggtcgcg ttcaggcgtt tatcgctacg ctggttatga tgcttttact gcgcggcgtg
    12661 accatggttt ataccaacgg tagcccagtg aataccggct ttactgagaa cgccgatctg
    12721 tttggctggt ttggtattgg tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg
    12781 attgtcttcc tcgcggcctg gtacatgctg catcacacgc gtctggggcg ttacatctac
    12841 gcgctgggcg gcaacgaagc ggcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa
    12901 atcatcgtct attctctttg tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg
    12961 cgtctctcct ccgcacaacc cacggcgggg actggctatg agctggatgc tattgctgcg
    13021 gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc
    13081 ggcgcattaa ttcttggctt ccttaataat ggattgaatt tgttaggtgt ttcctcctat
    13141 taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag
    13201 cagtaataac gactacagga catcttgaat atgaacatga aaaaactggc taccctggtt
    13261 tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg
    13321 ctggtggtct ccacgcttaa caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa
    13381 gaggcggata agcttggcta taacctggtg gtgctggact cccagaacaa cccggcgaaa
    13441 gagctggcga acgtgcagga cttaaccgtt cgcggcacca aaattctgct gattaacccg
    13501 accgactccg acgcagtagg taatgctgtg aagatggcta accaggcgaa catcccggtt
    13561 atcactcttg accgccaggc aacgaaaggt gaagtggtga gccacattgc ttctgataac
    13621 gtactgggtg gcaaaatcgc cggtgattac atcgcgaaga aagcgggtga aggtgccaaa
    13681 gttatcgagc tgcaaggcat tgctggtaca tccgcagccc gtgaacgtgg cgaaggcttc
    13741 cagcaggccg ttgctgctca caagtttaat gttcttgcca gccagccagc agattttgat
    13801 cgcactaaag gtttgaacgt aatgcagaac ctgttgaccg ctcatccgga tgttcaggct
    13861 gtattcgcgc agaatgatga aatggcgctg ggcgcgctgc gcgcactgca aactgccggt
    13921 aaatcggatg tgatggtcgt cggatttgac ggtacaccgg atggcgaaaa agcggtgaat
    13981 gatggcaaac tagcagcgac tatcgctcag ctacccgatc agattggcgc gaaaggcgtc
    14041 gaaaccgcag ataaagtgct gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg
    14101 aaactggttg ttaagcagta gttttaatca ggttgtatga cctgatggtg acataaatac
    14161 gtcatcgaca gatgaacgtg taatataaag aaaagcaggg cacgcgccac cctaacacgg
    14221 tggcgcattt tatggacatc ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca
    14281 gcattaatgc tgaccacatt cttaatcttc aatcttttcc tactccaggc gaaaccgtaa
    14341 ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg
    14401 ggcgtagcgg tgcgaatatc gcgtttattg cctgtacggg tgatgacagc attggtgaga
    14461 gcgttcgcca gcagctcgcc actgataaca ttgatattac tccggtcagc gtgatcaaag
    14521 gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga aggtgagaat gtcatcggta
    14581 ttcatgccgg cgctaatgct gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta
    14641 ttgccaacgc gtcagcatta ttaatgcagc tggaatcacc actcgaaagt gtgatggcag
    14701 cggcgaaaat cgcccatcaa aataagacta tcgttgcgct taacccggct ccggctcgcg
    14761 aacttcctga cgaactgctg gcgctggtgg acattattac gccaaacgaa acggaagcag
    14821 aaaagctcac cggtattcgt gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac
    14881 tgcatgaaaa aggtatccgt actgtactga ttactttagg aagtcgtggt gtatgggcta
    14941 gcgtgaatgg tgaaggtcag cgcgttcctg gattccgggt gcaggctgtc gataccattg
    15001 ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc
    15061 cagaggcgat tcggtttgcc catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac
    15121 aaccttccgt accgtggcgt gaagagatcg acgcattttt agacaggcag aggtgacgct
    15181 tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg
    15241 ttatcaataa agatcgcttc gtcagtgaag cgattaccgc caaagttgaa gcggcgatta
    15301 aagaactcaa ttacgcgcca tcagccctgg cgcgtagcct caaactcaat caaacacata
    15361 ccattggcat gttgatcact gccagtacca atcctttcta ttcagaactg gtgcgtggcg
    15421 ttgaacgcag ctgcttcgaa cgcggttata gtctcgtcct ttgcaatacc gaaggagatg
    15481 aacagcggat gaatcgcaat ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt
    15541 tactgtgcac cgaaacgcat caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc
    15601 ctactgtgat gatggactgg gctccgttcg atggcgacag cgatcttatt caggataact
    15661 cgttgctggg cggagactta gcaacgcaat atctgatcga taaaggtcat acccgtatcg
    15721 cctgtattac cggtccgctg gataaaactc cggcgcgcct gcggttggaa ggttatcggg
    15781 cggcgatgaa acgtgcgggt ctcaacattc ctgatggcta tgaagtcact ggtgattttg
    15841 aatttaacgg cgggtttgac gccatgcgcc aactgttatc acatccgctg cgtcctcagg
    15901 ccgtctttac cggaaatgac gctatggctg ttggcgttta ccaggcgtta tatcaggcag
    15961 agttacaggt tccgcaggat atcgcggtga ttggctatga cgatatcgaa ctggcaagct
    16021 ttatgacgcc accattaacc actatccacc aaccgaaaga tgaactgggg gagctggcga
    16081 ttgatgtact catccatcgg ataacccagc cgacccttca gcaacaacga ttacaactta
    16141 ctccgattct gatggaacgc ggttcggctt agatttacgc tgtcttttga tcaaattatt
    16201 accatcggtt gttttcagaa gcatgaacat tgctgctgaa gcaacagtaa taatgcccat
    16261 cgtgataaac gtatagtgga attgttcgac agtcgttgtg ccttccatcc cttcataaac
    16321 gcgaaggacg gccgcactta cagcaacgcc taaactaatc gacagttgct gcgtgaccgc
    16381 cagaacactg ttaccgctgc tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt
    16441 catcgcggta aattgcgtcg acatagccat ccctaatata aacaacggca agatcagcat
    16501 ccatatcgcc attgccggtg attgcaaaga gaactgagcg atcattagcc caataatcac
    16561 cgtgatcccc actaacgtat ggcgatagcc cagacgacgt aagactt
//
LOCUS       DAZUFB010000107        16582 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZUFB010000107 DAZUFB010000000
VERSION     DAZUFB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.107"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            334..555
                     /locus_tag="ACHY3M_003893"
     CDS             334..555
                     /locus_tag="ACHY3M_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4318717.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGGL"
     gene            552..1586
                     /locus_tag="ACHY3M_003894"
     CDS             552..1586
                     /locus_tag="ACHY3M_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4318718.1"
                     /translation="MKKPLVTRNEIAEAIALHTTCMPTREIPGAIANYFMITRGFYTR
                     TDKAVINKLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRVTGKNLNIEKPAPVTSATP
                     TPAVNVIPNIGDTIDSQTLLKMVNEARKLCSEKPVRNNDFIARVKDELEGETYEIFVG
                     QKNGAEIDIITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPEALR
                     LAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWLRQN
                     GILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRKLLD
                     AGLLKPVAIG"
     gene            1750..2550
                     /locus_tag="ACHY3M_003895"
     CDS             1750..2550
                     /locus_tag="ACHY3M_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4318719.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAAAR"
     gene            2580..3425
                     /locus_tag="ACHY3M_003896"
     CDS             2580..3425
                     /locus_tag="ACHY3M_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4318720.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQFKKTKRSVSGAAGTTKLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGVSNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            3476..3721
                     /locus_tag="ACHY3M_003897"
     CDS             3476..3721
                     /locus_tag="ACHY3M_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318721.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKEQAEEILV
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            3903..4058
                     /gene="hokD"
                     /locus_tag="ACHY3M_003898"
     CDS             3903..4058
                     /gene="hokD"
                     /locus_tag="ACHY3M_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4318722.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(4175..4684)
                     /locus_tag="ACHY3M_003899"
     CDS             complement(4175..4684)
                     /locus_tag="ACHY3M_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFS4318723.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTVAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(4696..5154)
                     /locus_tag="ACHY3M_003900"
     CDS             complement(4696..5154)
                     /locus_tag="ACHY3M_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318724.1"
                     /translation="MAQNWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWE
                     GGTPPTFTLPVTFIALFDPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRQ
                     INIIDVAIQDISFDLDAPRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(5313..6128)
                     /locus_tag="ACHY3M_003901"
     CDS             complement(5313..6128)
                     /locus_tag="ACHY3M_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318725.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(6138..7727)
                     /locus_tag="ACHY3M_003902"
     CDS             complement(6138..7727)
                     /locus_tag="ACHY3M_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318726.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYKDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(7788..9494)
                     /locus_tag="ACHY3M_003903"
     CDS             complement(7788..9494)
                     /locus_tag="ACHY3M_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318727.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFNV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(9719..10720)
                     /locus_tag="ACHY3M_003904"
     CDS             complement(9719..10720)
                     /locus_tag="ACHY3M_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFS4318728.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(10737..11933)
                     /locus_tag="ACHY3M_003905"
     CDS             complement(10737..11933)
                     /locus_tag="ACHY3M_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4318729.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(12102..12473)
                     /locus_tag="ACHY3M_003906"
     CDS             complement(12102..12473)
                     /locus_tag="ACHY3M_003906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318730.1"
                     /translation="MAYVSISGYEPDAIRAQCELLKAMFMLRTKYPWETGRVCFPMEE
                     LTATIDSQLYHDNPEKFFSLLAGNGLNCVSGLGRSRIQHASLFGPFFYDRHGYFEAEL
                     PALTRAIDTIDFERLFAALSK"
     gene            complement(12576..12911)
                     /locus_tag="ACHY3M_003907"
     CDS             complement(12576..12911)
                     /locus_tag="ACHY3M_003907"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4318731.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIK"
     gene            complement(13174..14058)
                     /locus_tag="ACHY3M_003908"
     CDS             complement(13174..14058)
                     /locus_tag="ACHY3M_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4318732.1"
                     /translation="MLEENKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            complement(14392..14784)
                     /locus_tag="ACHY3M_003909"
     CDS             complement(14392..14784)
                     /locus_tag="ACHY3M_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318733.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            complement(14796..14936)
                     /locus_tag="ACHY3M_003910"
     CDS             complement(14796..14936)
                     /locus_tag="ACHY3M_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318734.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            complement(14962..15384)
                     /locus_tag="ACHY3M_003911"
     CDS             complement(14962..15384)
                     /locus_tag="ACHY3M_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HFS4318735.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(15424..16212)
                     /locus_tag="ACHY3M_003912"
     CDS             complement(15424..16212)
                     /locus_tag="ACHY3M_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318736.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQALNTGSESS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAR"
BASE COUNT         4386 a         4036 c         3801 g         4359 t
ORIGIN      
        1 gcaatgctct aataaatttg tatttttaag tcgcgaatgc tatcttttcg catcatattg
       61 accttttaat cgttcaggct tatagttcct acgtcgtagc aaattctgcg accaggtttg
      121 acagcctgaa tgattgtgcg gacaaccgca gatttccgat attgcggtat ttttgtgtcc
      181 gtaaaaccac gttacgcccg aattatggtg gggcgtgatg gggaggcttc ggcctgctgg
      241 tttcacaatc gccagtctgt caaccctgtc acgtcctgcc acctgtttga cagcgggtag
      301 caggttgtta aacctgattg tgaggccgta actatggtta atgccaatcc ttgcgcacgc
      361 caagaattca tctggcgttt ctattcctgt aaaaaacacc actatcactt cgttatcgca
      421 gcaactgaag acgaagcacg ctctcaattg cctgatggcc cctgcatttt tactgcccgt
      481 ttttcaacta actcgcgcaa ttcacttagt tactggaacc tccccttctc tgccgatgtt
      541 caggggggtt tatgaaaaaa cctctcgtca cccgtaatga aatagccgaa gcgatcgccc
      601 tgcatacaac ctgtatgccg acacgggaga tccccggcgc aattgccaac tatttcatga
      661 taaccagagg tttttatacc cgaacagata aggctgtgat caacaagcta ctgatagccg
      721 agatcaggga ttatttgatt gaacaaggac gtctacgtta cgcaacagtg gcagcagaaa
      781 tgagaaagga ggcacacaga gtgaccggta aaaatttgaa tattgaaaaa ccagcacctg
      841 ttacgtcagc tacgccaaca ccagccgtga atgtcatccc caacatcgga gacacaatcg
      901 acagccaaac attgttgaag atggtcaatg aggcacggaa attatgtagc gaaaaaccag
      961 ttcgcaataa tgatttcatt gccagagtta aggatgaact tgaaggagaa acctacgaaa
     1021 ttttcgtagg tcaaaaaaac ggcgcagaaa tagatattat aacaatgacc tacaaacaag
     1081 ccctgcgagt tgccgcgcgc gagtcaaaag cggtccgccg ttcgctgatc gacaaactgg
     1141 aagaattgca gcaggcaaac tcacctaccc catcgatccc ccaaacatta ccagaagctc
     1201 tacgcctggc tgccgagttg gcagaacaga aaatgcagtt ggaacaacag ctggtggccg
     1261 cagcccctaa agtcgatttt gccgaccggg tatcagcggc taatggaatc ctgatcggga
     1321 actttgcaaa ggtcgttgga cttaagcaaa acgccctttt ctcatggttg cgccagaacg
     1381 gcattctcat ggcttttggt gcgcgcaaaa acgtaccgcg ccagcagtac atcaacgccg
     1441 gatatttcac ggtgaaagaa gtggtgctgg atgatgaaaa tggctaccag atacggctga
     1501 cgccccaatt aacgggtaaa ggccagcagt ggttaactcg caagctactt gatgctggtt
     1561 tgttaaaacc agtagcaata ggttaacaaa agaaaaaacc tgccagcaaa ctggcaggtt
     1621 tctgagcaga tcgaccaacc cgatctggat cgagtcagaa aaatttgctc taataaattt
     1681 cgttttctaa gtgcaaagaa tcaccatttc gagctggtga ttgaaggttg atgcaaattt
     1741 ggagaaaaaa tgcaacaaac attcaatgcg gatatgaata tatcaaacct tcatcaaaat
     1801 gtcgatcctt caaccactct gcctgttatt tgtggtgttg aaattacgac cgaccgcgct
     1861 ggccgttaca accttaatgc tctacacaga gcgagcggac tcggtgccca taaagcgcca
     1921 gctcaatggc taagaacgct gtcagccaaa cagctcatcg aagagcttga aaaagaaact
     1981 atgcagaatt gcatagtttc gttcgaaggc cgtggcggcg gcacttttgc ccatgaattg
     2041 ctcgctgtgg agtacgcagg ctggatttct cccgcgtttc ggctgaaggt aaaccagaca
     2101 tttatcgact atcgagccgg aagattacaa cctgctattc cgcagagtct ccctgaagct
     2161 ctccgtttgg ctgccgacct ggcagagcaa aagcaacggc tggagcaaaa aatgcttatg
     2221 gatgcaccta aagtcgaatt cgccgaacgc gttgctaccg ccagcggagt tctaatcggc
     2281 aactatgcca aagtgctcgg cctgggccaa aactatctct tcacctggtt gcgtgataac
     2341 ggaattctga tcgcaaccgg tgaacgcagg aacgtcccca aacaagaata catatcccgt
     2401 gggtatttca cccttaaaga aaccgtgatc gatacaagca atggaagcag gatttctttc
     2461 acgactcgta taaccggcaa aggtcagcag tggctgatga agcgattgct tgatgctggt
     2521 gtgctggtac ctgtcgcggc agcgcgctaa cagacgtagt aagaaccacc agcattgtaa
     2581 tgctggctaa agtcactttc ctgagctgta taacgatgag cgattttact ttttctggct
     2641 atgaattggc ctgctttgta acacactccg gtctatcccg tagcgccggg catatcctgt
     2701 cgcaatgtgc aaatctcgcg gcaacaacca gtgaatactt cattcacaag cctcaccgcc
     2761 tgatcgcggc agaaactggt tatagccaat caaccgtcgt tcgtgcattc cgtgaagctg
     2821 taaacaaagg aatcctgtca gtagagattg ttatcggcga tcaccgtgaa cgtcgcgcta
     2881 acctgtaccg gtttacacca tcctttttgg ccttcgcaca acaagcaaaa aatgcgctga
     2941 ttgaaagcaa attaaagatc tcttcagcgg caaccaaggt taaagctgtt ctcgctaaga
     3001 cattggcttt atttaatttt ttatccacac ccccatgtca aaatgatacc ccctccccct
     3061 gtcaggatga cgtggcaata aagaataaga agtcacaatt taaaaaaaca aaaagatcag
     3121 tttccggcgc tgccggaacg accaaactca aaaaattgac ttcatggatc gctgaggcaa
     3181 aagcaaaggc tgacaatctg cggttatcca aaaaacgcgc tcaaaaacat gagttcaagc
     3241 agaaagtaga ggcggcagcg cggaaatatg cttacctgaa gaacaagcgt tctcctgata
     3301 ttggcggggt atcaaacttc gataatctgc cgcattgcat gacggtaaac gaagctctta
     3361 atgcggtttt agccaaaaat aaagataacg aacaatgggg tataccggca ggattcagag
     3421 ggtgatagat tgctctaatc tggagtcacc tggcgttttc agtttgaggt cggagatgca
     3481 atctgatttt ttacagttag cgatcgcttt tgcaggatat gtttgtattg gcttctgtgt
     3541 atacatgatc agccgaaaaa tgcttgtcga tatcgaccgc aaagaacaag cagaggagat
     3601 cttagtatgg attttctttg gcgcggtctg gccattaggg atcatgtttg ctgcaacatt
     3661 tcttctgatg tggatattca cccttccagg tgatttctat agaaaaaaag ccagacattg
     3721 atacaatcgt tgcgggtgct tgaggctatc tgcttcaggc attacccgaa aagcagatag
     3781 aagaaagccc cagataacat tacgcgtcct gcaagacgct taacattaat ctgaggccac
     3841 atctatgctt agcatacgta gattagcctc ttaccgacca aaaggtcaag gagaagcagg
     3901 ctatgaagca gcaaaaagcg atgttaatcg ctctgatcgt catctgttta accgtcattg
     3961 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4021 aggtcactgt cttcacagcc tacgaatctg aaaggtaaga gacctggcgg ggagagatcc
     4081 ccgccactct tcgtgtgtca ggtatcctca atgcaccctt tcctctccaa ataaaaaagc
     4141 tcccgaagga gctttaaaat acaagggatt actattaatc ccactcaatc cagttgtaga
     4201 cgatacgaag tgacgggcgc acagcggcag tcacatcttc ggtactaaag tcgattgcat
     4261 cactgtagat tttgcagtcc aacatttcaa ttgttgtagc agcttttgtc acagcgttaa
     4321 ccccggaaga tttggattca ggggtagcag ccatcgtgat atcaacatag tccttcgccg
     4381 caatgcgatc cttgatgaac tgaagaatat cgccttcgat agtctccacg cactggacct
     4441 ggatttcccc agagtttcga attggtccgt gctggttgaa cttcacacca ttcggaccat
     4501 agtcctccac atcctcgcgg gtcatttcag gaatttgcga cgtgcgaacc agtacgctga
     4561 tatcttcatg gcctgcaaca gtgagctgga attcagaaga taccagtcgt tcgcctttgg
     4621 ccgcgttggc agtatagcgg cccttaataa atttacggtt tcccttagtg ttattgtgcc
     4681 ccatataaaa tccttttact ggaacgcccg aacaatatcg gagctgttat atatcgaaga
     4741 accggtcaac tggaggttga cggtgttttt caggaaatgc ccattgctgt ccctgggcgc
     4801 atcgagatcg aaacttatgt cctggatagc gacatcaatg atgttgatct ggcgaccaat
     4861 gtttagcgtc acacgctccg ggattcgacc accaatactg gcatctttaa gttccgggct
     4921 aatcatcgct gacaatgcgg cgatagctcc tgaaacctcc gtgaatggat caaacaaagc
     4981 gatgaaagtt actggcagcg tgaaagttgg cggtgttccc ccctcccaaa ccattaagct
     5041 gttccaacgg gccaccgacg ttgtttcagt accaacctgc gcaaaaccac tgaaggcacc
     5101 agcaacagac cccatggaca taccggtaaa cggcgcttcc caattctggg ccatgttcat
     5161 tgctgccccc tggctgatat atccggtaac ctggtactga gagttcgtta aagtaacttt
     5221 cagaaatggc gatacaccgt cagcctggct gtaaacccca taaggtatag gtgccattca
     5281 agttaaaggc cggagttctc cggcctcctc ctttagccaa ggcgcttacg gcgcagtttc
     5341 attgactttt tgcgggcaag ttttgccgca cccgtctggg cttttcgacg cgcttttttc
     5401 agcgccgatt tttgagccgc agtcagacgt tttttacgca ggcgtttacg gatgagtttg
     5461 atctcaccgt tacgaacaac cttcttaaat gcttcagtca gcatttcatc agaagtgcca
     5521 gcaacaacaa acgccgcttc cagttcgtca cggtcgtcgc tatctaaacc agcgatagag
     5581 gcaccaacat cagcagctgc gtcgtcgtct tcatcgtcag ccagtgcttc gatcaggtca
     5641 tcatctacac cgcatgctgc gaggaagtca gcaacatttg cccatgcttc gttataggca
     5701 tcgtcctgtt cttctgtaac ttcggagtcg tcgtcatcag agataccagc gatagcctga
     5761 acgaaaccat caagggagtc gaaagtcaga tcaccgctat cagcccaggc gaaaacggcg
     5821 tcggccgcat cactcaacgc attctgcata gcacttctat ttgcagcttc cagaatcatc
     5881 tggtgcgcct gttcgacggt ccattcttta ccgtctttcc cttccaggat ttgctcagga
     5941 gccggggcag atggaacgtt atcgttagtc tgtgccgccg gttccggatt attattaata
     6001 accggatctg ttggctgttc ggcgcttgct cgggcagact ccatcagctg cacaggatca
     6061 gagttcaaag cgaaacgaga cagtccattc cccaaaaatg ccccggattg aaaaaagttt
     6121 ttgctcattg tattccctta cttaataagc agcggtacgc cctggatacg acgggctacg
     6181 ccagtcgggc agcaggccca gactacttcc catttatcga attccgcctg cgtaactttc
     6241 agcacatacg gttctgtacc gtcagcatca ggatcacgag gagccaccag agcgccggag
     6301 gcgacaaagc gatctaaaag tttggtcatc cctttagtca ggccagccgc agtaataccg
     6361 tccgggctat gcttcatctg tcgggctaac tggacaaaga aacggctgat tgcattcatc
     6421 agggatggga cgtgctggaa gtgcagatag ttatcctgcg tgcagcaagt taaagcatcg
     6481 tcgatgatca tctggccaga ggtgccaaca gatactttat tgagacggcc cttgaccatt
     6541 gcttcttcgt ccggggtatc ttccggatac agcggttgaa ttgacgcacg agcaatgacg
     6601 gcacgttctt caccagccgg tgagtaatgc caaccgccga catcagagtt tttcttgacg
     6661 ccacgagctt tcgccgcata cgccacgcca gacagaccaa agaccacacg ggattgggtc
     6721 catttgtctt tgcaggagaa cgggtagtga tagacagcac agcttacata atcggtacca
     6781 agtaaaccgg tatcttcaac agcagagagc gcttccgtat acgtcaatgt cggtttgaca
     6841 tcaaagaagc catcaatcag gcgatcagaa cagatattac ctaacgcggt gatcgccgca
     6901 ttgtcatagc aacccaggcc gagaacagcg gtgtacatgt acggcgcatt gttcagcacc
     6961 ttaaccgcac gcaggtacgc agcggttgag attttcgact gatcaccgtt ggtaccacca
     7021 gtgaacgcca gcgatttttt atttgttact ttcgccgtcg aaatcagctc ttcattaaca
     7081 accgcgcgca gatatttaga acgggcttcc agagccgtag gcagataaca caagcggccc
     7141 atgtcatctt tcgcttcttc cgccaaagac acagtgtgtg tctccagggt cgttaccacg
     7201 ccgagcgaag tcgtctgggt cagttttaag aggaagcgtt cattacccgc gctgtccgct
     7261 gttgccgttt cgatggttaa ctcacgggta ggtgaaatac acggatcacc atcatcaacg
     7321 tagatagcaa aggcttcgcc actatcaagt tcaatttcag aaccgtatgg caacgcactg
     7381 taagccggtt cgcctgattc atcgaacata ataatcggga acttcgcatc atccggaaca
     7441 gcacgaacaa cataacctga cgtttgctga atagcttcgt atacatggcg aattggttcg
     7501 aactgtgagc cggaagacgg cttcagcggt tcgccgagaa catctttgta attggactca
     7561 gtaaccgcaa gaacagtaaa cggcttgcca cgcgcaaata cgccaatacc agcccacaag
     7621 ctgctattta atgcaacacc ggtagataac gtcgcatcgg cattgatcgg gctaaccgcg
     7681 acgccggatg cattacctaa tgactgttga attgaatatt gagacataac tttccctgtt
     7741 atgcgccccg cacgggggcg ctatgttaaa cggagaactt cccctgatta ctcagagtca
     7801 ccggcatcaa tcgtgtcgcc gcttatgaag ttaagcccgc cttttttggc cattgtcagc
     7861 gttacacgag tgaagtaatc agcgccgttg cgtgggtgca tatcgttgat agccgaaccc
     7921 cacagtgtgg tacggttgac cagcgccgga gtggtcggat gctggaacgg gatagccggg
     7981 acagcatcac cagtcacgaa gcctgcttta cccggatttt catcacggac gtagcacagc
     8041 acatccatcg agctgaactg aatgttctct gtcgttaagt tcttacaaat accagcaggt
     8101 acttcgtaca ctttcacgtt accgaacagg gtaccgatgt agtgaacata cggagtctgg
     8161 atatagtctt cggctggctg gaagaaatcc ttcggcaact gtttgaagaa agatgctgca
     8221 tcagcaccag caaacatccc catcgcacca gaagatttaa cgcgctcaat aatgtcgcga
     8281 tatacagtct ggaatttgcc acgaatgatg gttgcccata cattaaagga ctggttaacc
     8341 ggcagagcga tgtcaaaggt gtcggtcgca agagtacgcc agatcatgat gcgaagacgc
     8401 agcatatcct gttcatgaga caggtattcc ttcagggtgc ggaactgtag ggaacccagg
     8461 tccagaccaa attcacgctg tgcttcatac gccgcctgta ccgtgtgctc agccgcgata
     8521 acgaactgac ttgggaacag ggtgtatttc ttcatttcgt ggttgatcag cgggatcagc
     8581 tcaggtgcgg cttcaatatt gatttccgtc tcaattgcga tctcagtgcc tttatccggc
     8641 gctttggaga acgacagggc aatctgacca atgttgtagt tcagagagca ggtaacagtg
     8701 atttgctcac cagcagcatt agtaaacgag tgaagtaggc tgccggaacc gttatcaaca
     8761 acagacttaa tacggttaac gtagatgtta gtgcgacctt ttcggattgg tacattctgg
     8821 ccttcgaagt cttccatctt gaaggttgcg gttttgctgg tgccatcgga gcttgccacc
     8881 agcacatagc ggcgacgtaa ctggctgtac acaccaacgg attgcatgtc cagaacatca
     8941 ccagcagcat aagaaccaaa agaggaacct gccacgttaa agacttcata gatgtcggac
     9001 tggtcacgcg taaccggaat gaaggtacac gcatcagcgg tagctgcccc caactgaaca
     9061 ggcaggatca tcgcgaggaa taaaggcaga cgcataacac cgtcagaaac gctcatcatc
     9121 tctgctgcga cggattccag catcgcttta ttagtggcat ccatgctatt gcgggtggac
     9181 tcaatcaggc agttttccag cgtctggtgg caggaggcca gaatttccgg acgcggcata
     9241 gatttatgtg ctgcggcgta gtcagccagt gcacttgccc acgctgtagc gatttgagcg
     9301 gtggcattat cagagatacc cgcaaaaacc gggtctttac gtgcagcttc aaggatagat
     9361 gcggcacgcg cggcatcatc tttaatgaat tggttatcag taccgaactg cgcagtgctt
     9421 gcccagccaa gcacagcttt agagcgtttt gcgatatctg caatacgatt ctggtattcg
     9481 cgtaagttac tcaatttact cttccttaaa cacaaggcac ttgtgtgaat cccttttcgg
     9541 aagagatttt attgaaagtc acttgttgac tttctcgtga caagcaattt ttttattttt
     9601 tcgggagtag gggaggaagg taaaatccaa ggtgaaatcg tggcgatttc accttgaaat
     9661 tttagatgga tttactttaa aaacagtagg ttaatagtga aatttgaatg gcgaaagttt
     9721 aaggcttcgg ctttttatcg aggctctttc taaggatatg cccaatcatc ctgtcgagtt
     9781 cttcctgtag ctcttttgaa agtcgattaa actcataaga aaatgcacgg cctttcacgc
     9841 gcttccttgc aaagcgatcc ttgtcctcaa atttccataa ttcagtaact acggacttat
     9901 ctttagaacc tttatccgtg agtagtgagg cttcctttgt tatcaagcgc aggattttat
     9961 ttttaacttc atcttcggcc atttcttcaa tggataagat gtcgtttatt tccggggata
    10021 tgttttgaat aagctgatca aactctaaat tcttgttccc catttcgtcg ccaacagcac
    10081 aaagcgtttt gtagtccgaa aaggttaatt ccgactgcac agggaaaagg gcgactaatt
    10141 cttccggagc actcgctgcc tggagagcac gcgtgacctt cgcctgagac agcccttctt
    10201 tggctgcaat atccttctga ctcatcccat catttttcat tcgcatcaaa cgcagaccta
    10261 tttctcgaat gctgtgctgc aatgctgtct gaacgtcttt cgctaaattt tgcgcttcct
    10321 gaacgctgat ctcctggtcc gtgactaaaa cccgcaaccc tacgttctct aagatggcag
    10381 aagctcgacg ccgggaacca tccaaaattt caattttccc tgtagcccgt ctaacaccta
    10441 ttgcagggta aaattgctga tgcttaatag tgcttcggat actttttaat gattttggcg
    10501 taagagatgc ctggtcacgc ccgttgttat gctgatcaac aaaggtatcg ctttctacct
    10561 ggttcggagg tattacctct tcaataaatg tggcctggcg accagttgat aacttgaata
    10621 cctgctcgac tcgatcgcca gaggctgaag aactatcaaa tccgcttaat attgaaggat
    10681 taagggttcg cccaattgtt ggtctgtttt tctttgacat gggggtttct tactcctcag
    10741 ttagatctga taaattcaat acggtcaaaa actgctttag caaaatcttc cgcggcaatt
    10801 cgcgcgttct tcaatgcatc agcactacca acatacgttg cagggttagc tgaaataaca
    10861 gtgtcaaaag actcgccgca gcgttcaaaa ccgtcaaggc gagggaggac gacatcaagc
    10921 atatccccac cgaacacttc tttagccagg ctatggcaat acttatggtc tgccttgtta
    10981 ctcaacttgg acataaaacc aatgttagtc gcaagctggc actcgcagcc ttcatccgaa
    11041 atgagtttca ccaactcagg aaggcgggca acgtatttaa gcgatgagtg gaaatcaacc
    11101 gttgcaggcg gcagaggtgt aaacagtata ttggccgagg ccaaagcatt tttcaggaag
    11161 gcgtcaaggt gaggaccact atcaacgagg ataaagtcat aatcgctctt cagcttatca
    11221 atcacatttt ctttcaggac agcatggatg ttctgacccg gtagatgctc attgcacagc
    11281 tctctccaat cggatgcaat aaaggcatcg tcaatcgacg caggcataac gtcaacccca
    11341 ggtacaacag aaggaacaat aaactcctct aacagctctt cacggcttac attctgcaac
    11401 atagcctgtg cagatgttgc gtttacgata ccaatagagt gtttatggct taaaaacatc
    11461 gttgctgaag attgcggatc aaggtcaata accagaatcc ttaaatcttc catcagaaga
    11521 tgagggtgag cacgcattgc atgcgccaga gaaaccgtcg atacagtttt tgacacaccg
    11581 cctttaagat tggagatgaa aatcacatac gcttcgctgt agcgatcccg gtattttggc
    11641 actccgcgat gttcatatat gtcaatgatg ttctgaattg acatcgcata tttcattgaa
    11701 gagccagcag ggcgtttatc gaaaacataa cccttttctt ccatttcact tacggcatag
    11761 tcaacgttcg ctcgagtcag tagaggcaat tttgccagtg ccgctttcgc atagacctgg
    11821 taaaactcgt tcgcgtgtag ctcatccttt tgcaactgta cttgttcagt cagaacattg
    11881 agcattctgt ttgctctttg agcaaccttg tgaagctggc tggaatcact catcgaaagt
    11941 catcctttat gctgtatttt tgaatttaat taaaaatgct gcataaaata ataatgtatg
    12001 cgtagatgct tgtacatggc attctctgca tgttttgttc attttgcacg attgagagtt
    12061 acaagaaagg cacaaaaaag ccccgttcag gggcatcagt gttatttgct aagagcagcg
    12121 aataatcgtt cgaaatcgat agtatctata gcacgcgtaa gcgccggaag ttcagcctca
    12181 aagtacccgt gtcgatcgta aaagaaggga ccgaagagcg aggcatgttg gattctactt
    12241 cgccccagcc cggacacaca gttaagccca ttaccggcta aaaggctaaa aaacttctct
    12301 ggattatcgt ggtaaagctg agaatcaatg gtggcggtta actcttccat agggaagcac
    12361 acccgccctg tctcccaggg atatttagtc cgaagcataa acattgcttt cagcaattca
    12421 cattgagcgc ggatcgcgtc cggttcatag ccagatatgg agacataagc cacgtccctc
    12481 attcctgcgt catctttgaa agtcacgata gaagtaacat ccagttcttt ttcgaaagag
    12541 cgagcagcat ctactggacg ctgaagtaaa tcattctact taatgcgctc gagaatccct
    12601 ccccacatat catttagata ttcgatatga gccaaaacct tatcaagaca ctctcgtgta
    12661 aaccattcag tatgcccgcc accggcgctt ttctcccacg gcgcattcca ggggaaaaag
    12721 gttgcgtgta aagcccgctc aagattaacc attgccaaac gcgtaccacg atagacccgt
    12781 gaaagcgcaa aatcgggact cacttgtagc cctttaaacc gtgccaatgg accacatgaa
    12841 atgccgattt taaaagtatc tccgttctcc ggcaccagaa cgtagaggta gtgttgtttc
    12901 tcttcttgca tatcaatacc actgcttgat gagaaccgcg caaatgttga ctatgcgcga
    12961 tgtgactaca gtcaaaagtt gactgtagtc gatttaactc caccaaagat cgactatgta
    13021 agatattgtc gggagaaacg ttgactatac gcgatgaaat gaccctaaaa gccatctcaa
    13081 tagcgacttg cagaatattg acgccagcaa aaatccacca gcgtcaacga atgtcgccta
    13141 tagtcaactt ctcgctatcg catatagtca atattatgga ttgcgcttat ggatctgaaa
    13201 gccgattttc ctgccgtttt ttatctctga aaatttaaga tattcaatag cttccaaatc
    13261 tttcatggct tttctgataa cgctattttg cacgctaacg gatgatttga gattaagcct
    13321 tgctctaagg cgctcaatgc tgacaggtgc cgggttggcg ggtagagcct caaagaatgt
    13381 atacagtacc ttggccgtct ctttgcgccc gagcttatcc agcatcttca gcttcaggat
    13441 tcgcttatag tcaacatagt aaagttcaga tagctgtttc tgcggctgga tctcgataac
    13501 atcaagctcg gtattcaggc tgctatatgc caacaagttg acgttaatgt tattgagatg
    13561 accttttgcc gccgggaagc ggaatttgac aactgtctgc tgaatgcgtg tcagagagtc
    13621 atcaatactt ttacggaacg cctttgaaag gcgcttacgt ggatagccgc atcgatcggc
    13681 aaactcggag aatggcaggg tgattatgcc gtcatcatca ggtgcgtagt caaacaacgc
    13741 ggaggttatg cccacccaca ccttaaaatc agtatccata tccaggcgtg gaccatgaat
    13801 ttcaattccc tcatagcctt cctgctcaac aattttgagg cttgatagtt cttcggttgc
    13861 gttcgttgtg tttgtagtaa ctgacgatcc gcgacgtagt gccacattgg tagattttaa
    13921 ggttggcaca aacacaccta agcgcaacaa agcaatgggt tgtatagtgc tattgttatt
    13981 gggtttcagg ctgtggattt ctcctgtatt tcctgcaact tcttcaacgc taaggaagcc
    14041 tttattttct tccagcatcg tggtttctcc atatgtggcg cggcctggcg tcatctggat
    14101 tgcagttatc aacaactgtg aatagtcagg ctctaaaatc gcgtacagtc aatgtttctg
    14161 tcgcatatag tcaacaataa atcgcgtgca gtcaacaata aatcgtgcac agtcaacata
    14221 aaatcgcgta tagtcaatgt tgatcccatt tcaggccaga aatggcgcgg cttacagcga
    14281 tccgggatct tctttggatc ttcctgggtt ctctttagga tctgtttatt ggatctatgc
    14341 tgtggataag ttgaataaac cggccaacat agccggttgg aaggaagggt attattctac
    14401 gctttcgata agaagaccat gttcataaca tttaagctca tcgccttcgt acaggaattg
    14461 gtatccaata ccaccatttt catggacatt agggaataac tcataactca ctgaagagca
    14521 aatcacacca atgcagcgat caacgccttc tcgttcttca gtgctgaaaa aatcctcttc
    14581 ggtaagaaca tgagtacatt gctcatcagc ataggtcgga aatacatgct cgatgcaatc
    14641 cgggtgtttt aaaccaagct gatcggcaag ctcgaaagca tgacggtatt gttcagatcc
    14701 tggcttgcca acagtgatgt gctcaatttt gtaaattgaa gtcgctttgt tgatagtttg
    14761 ctttactgtt actttatcag acataaaaat cccttttagt taccgctgat agcgcggttg
    14821 taatcattaa cgttgcgatt cttcctgtta atccccatca gcatcgtttc tgtatcgagg
    14881 atatacgctg gcagatcatc aaaatattca ctgctaaact ctggcatcct gcacataaac
    14941 gcacttttgg gggcagggtg gttaaccttt gtcggcgtcg gcgttaaatt cgctgatcga
    15001 ctcccggagc aaccgctgag tgtcagcagg aatgcgctgg cgaacattac ccgccgcaac
    15061 cagttgtttc tgaacttcag cttttcgttc catttgcctg tcagcatatt tggcttgttc
    15121 tgattcattt ttcacttcct ggctgtgaaa atgttgctct gctttgttca tcgtctcaat
    15181 ggtctggtta agatccatta ttgacttatc acgttcctta acagcctgat caagactgcc
    15241 aattttctcc atggcttgct ttagctgatg acgttcccat gcaaacccag caccaacaag
    15301 tgcgcaaatc agaacaagaa caccagtagc agcaagtttc tccttcaaag acaaagctgt
    15361 ttttaacgta gaaaagaatg acatgtcttc ctcctgaaga aaaattatca atgaagtcct
    15421 ttgttaccgt gccgctttgt ttaattcatc aagaacagaa tcaggaacca aagcggcgac
    15481 tgcgctggct gtgctggcct tatttgctga tgcttccgca agcgcggtac cgatagcatg
    15541 gttataagca gttatggcta cgttggcgct ttcattcgct cgttcatact gctgttgtaa
    15601 cgcagttgtg ggtgctgttg tctggttgaa aacaacccca aactgttcag ttgctacttt
    15661 cagagattca atttgctctt ctgttagtgc tggtggggga gtggcagtgc cgccgcctga
    15721 accagagcct gacgagcttt ctgagccagt gttaagggcc tggttaatct cccccattgc
    15781 agcgactaaa cttgatgtat taagcgcgtt aacagcgtcc tcaagcgatt tagtaatatt
    15841 cacatcacca atggcaatag agatcggcag ttctgaaact tctcgctcat tagcacggca
    15901 gtaaacatcc caaccaatat cgagttgaag cagcattgac agatctgcat aaccagccaa
    15961 caggtccgcg tgcttagttg ccagttctcc aatgttcgtt aagccggttg tggttgttct
    16021 gatcgttgaa acatagctgg taatagtgtc gggatagaca attgtatcca gaattaatcc
    16081 ggtcaattct tctgcaagca gttttgctgt gttagcactg tttcgtgccg atgttatggc
    16141 accaggtgtt ttcatcccac cggcggcggc caatttttta tatgcggata actggtagtc
    16201 tttttccagc atgatatctc ctaacttacc tgaaccaggc cgtctccgga cgctacggta
    16261 gatccgcatg aaacagggtc accaacgcat acgatccctt taccattgac ggtaaaccat
    16321 gccctggttg atatagcttg cccaccgtgc gtactgtttc catcggtatg ctgtgcatat
    16381 tgcttaccat caactaacac ttcgactccg ttgactttaa gtagtggttc actctctaca
    16441 ggaggcctgg atgggaatcc tccgtgcccc gaacaaatgc tgtcttttgt tgcaatgctt
    16501 gccacgccat caccaattat ttgctctgat tttcgttatt ttaacttagg ttatttgtgg
    16561 tctgtatggc gtttacttat cg
//
LOCUS       DAZUFB010000108        16295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZUFB010000108 DAZUFB010000000
VERSION     DAZUFB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.108"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..88
                     /locus_tag="ACHY3M_003913"
     CDS             <1..88
                     /locus_tag="ACHY3M_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS4318737.1"
                     /translation="HNALDDARYQTKYVSVIWQKLIPSQADF"
     gene            150..419
                     /locus_tag="ACHY3M_003914"
     CDS             150..419
                     /locus_tag="ACHY3M_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4318738.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            388..1506
                     /locus_tag="ACHY3M_003915"
     CDS             388..1506
                     /locus_tag="ACHY3M_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4318739.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWKKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1673..2467
                     /gene="potC"
                     /locus_tag="ACHY3M_003916"
     CDS             1673..2467
                     /gene="potC"
                     /locus_tag="ACHY3M_003916"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFS4318740.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2464..3510
                     /gene="potD"
                     /locus_tag="ACHY3M_003917"
     CDS             2464..3510
                     /gene="potD"
                     /locus_tag="ACHY3M_003917"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFS4318741.1"
                     /translation="MKKWSRHLLAAGTLALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPAYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3568..4029
                     /locus_tag="ACHY3M_003918"
     CDS             3568..4029
                     /locus_tag="ACHY3M_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFS4318742.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4026..4814
                     /gene="ycfZ"
                     /locus_tag="ACHY3M_003919"
     CDS             4026..4814
                     /gene="ycfZ"
                     /locus_tag="ACHY3M_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFS4318743.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(4953..5774)
                     /gene="cobB"
                     /locus_tag="ACHY3M_003920"
     CDS             complement(4953..5774)
                     /gene="cobB"
                     /locus_tag="ACHY3M_003920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFS4318744.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5790..6701)
                     /gene="nagK"
                     /locus_tag="ACHY3M_003921"
     CDS             complement(5790..6701)
                     /gene="nagK"
                     /locus_tag="ACHY3M_003921"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFS4318745.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6730..7974)
                     /gene="lolE"
                     /locus_tag="ACHY3M_003922"
     CDS             complement(6730..7974)
                     /gene="lolE"
                     /locus_tag="ACHY3M_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFS4318746.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(7974..8675)
                     /gene="lolD"
                     /locus_tag="ACHY3M_003923"
     CDS             complement(7974..8675)
                     /gene="lolD"
                     /locus_tag="ACHY3M_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFS4318747.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8668..9867)
                     /gene="lolC"
                     /locus_tag="ACHY3M_003924"
     CDS             complement(8668..9867)
                     /gene="lolC"
                     /locus_tag="ACHY3M_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFS4318748.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10129..11202
                     /gene="ycfT"
                     /locus_tag="ACHY3M_003925"
     CDS             10129..11202
                     /gene="ycfT"
                     /locus_tag="ACHY3M_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4318749.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11330..14776
                     /gene="mfd"
                     /locus_tag="ACHY3M_003926"
     CDS             11330..14776
                     /gene="mfd"
                     /locus_tag="ACHY3M_003926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4318750.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRHTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            14920..15882
                     /gene="ldtC"
                     /locus_tag="ACHY3M_003927"
     CDS             14920..15882
                     /gene="ldtC"
                     /locus_tag="ACHY3M_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFS4318751.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(15965..16222)
                     /gene="bhsA"
                     /locus_tag="ACHY3M_003928"
                     /gene_synonym="ycfR"
     CDS             complement(15965..16222)
                     /gene="bhsA"
                     /locus_tag="ACHY3M_003928"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFS4318752.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4126 a         4185 c         4089 g         3895 t
ORIGIN      
        1 ccataatgca cttgatgacg cccgttacca gacaaaatac gtttcagtta tctggcaaaa
       61 actgatcccg agtcaggctg atttttaatg ttcaaccgtc gccagttgtc gttgatattc
      121 tgcaactggc gcgttccgga gtgatagcca tgagcgaaca gtacctgata acgctcgacg
      181 agtggaaacc aaaacggttc agtctcccaa taacaaacac taccctggtg aaatacggaa
      241 aactaggata catcgttcca agaccacaaa aaattcgtgg gcgttggctg atagatcgcc
      301 gagcagtatt tgttgggcct ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg
      361 atgatgcact gaaggagatt ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac
      421 gtagccggtc tttacgaaaa gtttgatcgc agaactggca gagtttacta ccagtataaa
      481 aatcctgtga ctggaaaatt tcacggactc ggaacagaca aaggtaaggc agaaaaaatc
      541 gcttccacag ccaatcagcg gatagctgca gcagaagctg aatatttcat gcgcaaaatt
      601 gatgaaagtc cgtcagcaac aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga
      661 tatctgaaaa tacaggacac gcgactgaaa aatggagata ttgcagctac aactcacaaa
      721 gaaaaaactc gaatggctgc atacctggtt tcccgtctgg gaaaccaccc attgaaagaa
      781 ctggaagtaa gagactttgc attaatactg gatgagtggc tggataaaga catggtcagc
      841 acagcgagag taaatcgtgg attatgggtt gatatttata aagaagcaca gcatgcaggg
      901 gaagttcctc ctggatggaa tcctccggag gctacccgta aaccgatccc taaagtaacc
      961 agagccaggc tcaccatgga agactggaaa aaaatttaca atgcaacgcc tgaaaaacac
     1021 tttatccgta acgcaatgct tcttgcgatt gttactggtc agcgccgtga tgacatttgc
     1081 cacatgcgtt tttcagatgt gtggaacgaa cacttgcata tcacccaggg aaaaaccgga
     1141 atgcgtctgg cgttaccgct tacactacgc tgtgatgcca ttgggataac gttaaaagaa
     1201 gttattgatg ggtgccgaga cagaatatta agtccatatc taatccatag tcggcaccag
     1261 aaacaaccga agccgatgag taaagacaac ctgagcgact actttgccaa agcacgggat
     1321 ctggctggga taattccacc agcaggaaaa actccgccaa catttcatga acaacgttct
     1381 ctatcagaac ggctgtatcg tgcacagggt atcgatacaa aaacattact aggacataaa
     1441 gtccaggcaa ccaccgaccg ctataacgat actcgaggtc aggaatgggt taagttggtt
     1501 atttgacaaa caccatatgg aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg
     1561 cgtggccttg ttttggggaa aagttttgga gagattttgg agaaaggaaa aaattgaata
     1621 aattcaaaat cctgaaagtg ctccacctta tgaataaaaa ggtggaactc gaatgatcgg
     1681 tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat
     1741 tattttgatt gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac
     1801 caccaaatgg tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc
     1861 actaacaatg gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt
     1921 tgcgctgtac cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt
     1981 gatgatgtca ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg
     2041 tattcagctt ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt
     2101 ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa
     2161 agatctcggt gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc
     2221 tgcggtggcg gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc
     2281 ttcgttcgtc accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa
     2341 agtcggcgta tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt
     2401 gatggttatt gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt
     2461 taaatgaaaa aatggtcacg ccacctgctc gcggcgggta ctctggcact gggcatgagc
     2521 gccgctcacg ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg
     2581 ccaggactgc ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac
     2641 gagtcgaacg aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg
     2701 gtggttcctt caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc
     2761 gacaagtcga agttaacgaa cttcagcaat ctcgatccag atatgctcaa caagccgttt
     2821 gacccgaata acgattactc cattccgtat atctggggtg cgacggcgat tggtgttaac
     2881 ggtgatgcgg tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgcgtac
     2941 aaaggcagcc tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag
     3001 ctgggctact ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg
     3061 aaaaaactga tgccaaacgt cgcagcgttt aactccgata acccagctaa cccgtacatg
     3121 gaaggcgaag ttaacctcgg catgatctgg aacggttctg ctttcgttgc acggcaggcg
     3181 ggtacgccaa ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg
     3241 gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg
     3301 cgcccggatg tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg
     3361 gcgcgtaagc tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa
     3421 accattaaaa atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag
     3481 tattatcaga agctgaaagc aggacgttaa ttcagcaagc gggcggtggc attaccgccc
     3541 gttttttatt tagcaaggat gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct
     3601 tttctcggta tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt
     3661 acttttacca aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac
     3721 aatcactcaa acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct
     3781 acgccagatt acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc
     3841 gaaggtgaca gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac
     3901 aatccctggg ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt
     3961 agtgtcggtc tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc
     4021 tgcccatgaa aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa
     4081 ccagtaaacc attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac
     4141 cggatgctga agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac
     4201 cgtttattgt gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag
     4261 cgatgctggc gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat
     4321 ttactacccg cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg
     4381 acgataaaca tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc
     4441 tctggtatca gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac
     4501 cgacgccgcc actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg
     4561 gcagcaccaa cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga
     4621 tcttttgcta cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg
     4681 ccaatatggg ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa
     4741 tatcgccact ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga
     4801 cgagtaaacg ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa
     4861 cgccttatcc ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga
     4921 taagcgtagc gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca
     4981 aactcaggca ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc
     5041 tgacttggtt cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca
     5101 aacccagccg ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc
     5161 gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc
     5221 gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg
     5281 gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata
     5341 tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc
     5401 aaaaagcgat cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc
     5461 tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca
     5521 ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg
     5581 ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt
     5641 acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc
     5701 tgacgcaaac gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc
     5761 cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca
     5821 cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt
     5881 aagagatgac gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag
     5941 ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc
     6001 gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa
     6061 agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca
     6121 aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt
     6181 aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac
     6241 tcgccggtaa tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca
     6301 acgccggtgc cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag
     6361 gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt
     6421 gcgctcaggt cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc
     6481 gtaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca
     6541 aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg
     6601 tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca
     6661 agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat
     6721 gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc
     6781 cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc
     6841 cagccagtgc aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg
     6901 atgaccgatc aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac
     6961 accgataatc accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa
     7021 gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact
     7081 cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac
     7141 cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc
     7201 gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg
     7261 taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga
     7321 acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag
     7381 ttgaccactc aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag
     7441 tttatgctcg ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt
     7501 cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg
     7561 ccacgcatcg ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt
     7621 aacgcccttc acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt
     7681 gatatacggc gcggcagcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg
     7741 ccagttagtc cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat
     7801 acggttgttc agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc
     7861 cacgccgagg gcaatgccaa tggtagaaat aacggagatc agcgacacca tgccgccgcg
     7921 ccgccgtccg cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc
     7981 gcccccatca ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc
     8041 atacgtttcg ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga
     8101 ttcaattccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta
     8161 ggttcatccg ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc
     8221 tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgatc cagccccacc
     8281 gcttttaaca tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc
     8341 agcggcatag ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa
     8401 ataaagccca gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc
     8461 ggttgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc
     8521 agcaaggtgc ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg
     8581 acgctgaaac tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg
     8641 cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt
     8701 ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag
     8761 cgcaataaca atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag
     8821 gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc
     8881 accgaggatc gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat
     8941 ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac
     9001 catcagccct agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag
     9061 caagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg
     9121 ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag
     9181 cggctcatcc agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg
     9241 cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa
     9301 agtgccaatc acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct
     9361 ggcagatggt accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag
     9421 ttgttcgccg aggataacat tatatttccc cggctcgagg tcagtttgtt tcacattgac
     9481 cagatacggt gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc
     9541 cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac
     9601 gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga
     9661 agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc
     9721 gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga
     9781 aagccaggag acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc
     9841 aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga
     9901 gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat
     9961 ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc
    10021 ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata
    10081 acccgcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa
    10141 gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg
    10201 gtcattacct tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc
    10261 aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt
    10321 atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat
    10381 aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg
    10441 ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc
    10501 gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg
    10561 tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg
    10621 ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg
    10681 tggtggggaa tgaacagtgt gatccgcaat ttgctttatt acagccttgg cgcatggttt
    10741 ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct
    10801 tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg
    10861 ctggtatcga ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg
    10921 cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat
    10981 cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg
    11041 tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt
    11101 actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc
    11161 tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg
    11221 gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga
    11281 tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca
    11341 atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg
    11401 cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact
    11461 cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga
    11521 tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca
    11581 tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg
    11641 cgtactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca
    11701 cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca
    11761 actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac
    11821 gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt
    11881 ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga
    11941 ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat
    12001 tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat
    12061 ttaccagcaa gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt
    12121 cttcagcgaa ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa
    12181 tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga
    12241 gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt
    12301 ggacgagctc ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt
    12361 accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca
    12421 ggcacaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc
    12481 ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg
    12541 aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta
    12601 tctgatgatt ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat
    12661 ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccacac
    12721 catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt
    12781 ccatctggag cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat
    12841 tactggcgag tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc
    12901 gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa
    12961 acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt
    13021 ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa
    13081 acacgatcgt gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga
    13141 tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg
    13201 tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct
    13261 ggcagtagat aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca
    13321 gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc
    13381 ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat
    13441 cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg
    13501 cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gtattaaagc
    13561 gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa
    13621 tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct
    13681 ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg
    13741 tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa
    13801 agccgccgaa cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca
    13861 gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt
    13921 gctggtttgt acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat
    13981 cattgaacgc gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg
    14041 acgttcacat catcaggcat atgcatggtt gctgacgccg catccaaaag cgatgactac
    14101 cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc
    14161 gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag
    14221 cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga
    14281 tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt
    14341 cgagctgcgg atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt
    14401 gtcgttctat aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt
    14461 cgagcttatc gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc
    14521 ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg
    14581 cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct
    14641 gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga
    14701 tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga
    14761 gaacgcgatc gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac
    14821 aaattctttg cacttccctg cactatccga cactgtcacc atccataatt caggcttatc
    14881 tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt
    14941 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    15001 gccaacacct ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat
    15061 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    15121 ctcgctctgt tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg
    15181 ttaacgatcc cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat
    15241 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    15301 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    15361 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    15421 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    15481 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    15541 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    15601 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    15661 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    15721 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    15781 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    15841 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    15901 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    15961 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    16021 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    16081 ccagatttgt ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg
    16141 actgaacttc gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga
    16201 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa
    16261 ttacttaacg atcgataact aaatgataga tgtga
//
LOCUS       DAZUFB010000109        16231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZUFB010000109 DAZUFB010000000
VERSION     DAZUFB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.109"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..1394
                     /gene="hpaG"
                     /locus_tag="ACHY3M_003929"
     CDS             105..1394
                     /gene="hpaG"
                     /locus_tag="ACHY3M_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFS4318753.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMYYVTGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPIL
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1391..2857
                     /gene="hpaE"
                     /locus_tag="ACHY3M_003930"
     CDS             1391..2857
                     /gene="hpaE"
                     /locus_tag="ACHY3M_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4318754.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2859..3710
                     /gene="hpaD"
                     /locus_tag="ACHY3M_003931"
     CDS             2859..3710
                     /gene="hpaD"
                     /locus_tag="ACHY3M_003931"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFS4318755.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3720..4100
                     /locus_tag="ACHY3M_003932"
     CDS             3720..4100
                     /locus_tag="ACHY3M_003932"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFS4318756.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4168..4971
                     /gene="hpaH"
                     /locus_tag="ACHY3M_003933"
     CDS             4168..4971
                     /gene="hpaH"
                     /locus_tag="ACHY3M_003933"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFS4318757.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4982..5770
                     /gene="hpaI"
                     /locus_tag="ACHY3M_003934"
     CDS             4982..5770
                     /gene="hpaI"
                     /locus_tag="ACHY3M_003934"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFS4318758.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5840..7216
                     /gene="hpaX"
                     /locus_tag="ACHY3M_003935"
     CDS             5840..7216
                     /gene="hpaX"
                     /locus_tag="ACHY3M_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFS4318759.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7226..8116
                     /gene="hpaA"
                     /locus_tag="ACHY3M_003936"
     CDS             7226..8116
                     /gene="hpaA"
                     /locus_tag="ACHY3M_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFS4318760.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8367..9929
                     /gene="hpaB"
                     /locus_tag="ACHY3M_003937"
     CDS             8367..9929
                     /gene="hpaB"
                     /locus_tag="ACHY3M_003937"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFS4318761.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQLDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9947..10459
                     /locus_tag="ACHY3M_003938"
     CDS             9947..10459
                     /locus_tag="ACHY3M_003938"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFS4318762.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10852..13002
                     /gene="btsT"
                     /locus_tag="ACHY3M_003939"
     CDS             10852..13002
                     /gene="btsT"
                     /locus_tag="ACHY3M_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFS4318763.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13052..13255
                     /locus_tag="ACHY3M_003940"
     CDS             13052..13255
                     /locus_tag="ACHY3M_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS4318764.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13266..14222
                     /gene="yjiA"
                     /locus_tag="ACHY3M_003941"
     CDS             13266..14222
                     /gene="yjiA"
                     /locus_tag="ACHY3M_003941"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS4318765.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14300..>16231)
                     /locus_tag="ACHY3M_003942"
     CDS             complement(14300..>16231)
                     /locus_tag="ACHY3M_003942"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFS4318766.1"
                     /translation="LKWLVNPSAPTTIAVQESSYPLKFNQQNSQTAALLPRYDLPAPM
                     LDRPAKGADGALLALTAGKNRETIAAQFAQGGANGLAGYPTTSNMWVIGKSKAQDAKA
                     IMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFGHNGVISWGSTAGFGDD
                     VDIFAERLSAEKPGYYLHNGKWVKMLSREETITVKNGQAETFTVWRTVHGNILQTDQT
                     TQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQALTINWYYADVNGNIGY
                     VHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNPLSGYIANWNNSPQKDY
                     PASDLFAFLWGGADRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDKHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         3765 a         4455 c         4344 g         3667 t
ORIGIN      
        1 ttagatcaca tttacatcac ttacttttgc caaactacgc aatcgggaat aaaagtaatc
       61 attaacatat taatgattaa aatgatcgaa tccgaggagt ggtaatgaaa ggcactatct
      121 tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg ttccagcaat
      181 ccccctacaa agccccgcct aaaactgcgg tctggtttat taaaccgcgc aatacggtga
      241 ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa ggtactgagc ggtgcgactg
      301 ttgcgctgat tgtgggaaaa acggcgacga aaatacgtga agaagatgca gcagagtaca
      361 tcgccggata tgcgctggct aacgatgtca gcctgccgga agagagcttt taccgcccgg
      421 caatcaaagc aaaatgtcgt gatggattct gccccattgg cgaaaccgtg gctctcagca
      481 atgtcgataa tctgaccatc tataccgaga tcaacgggcg tcctgccgat cactggaata
      541 ctgccgattt acaacgtaac gccgcacagc tgctgagcgc cctgagcgaa tttgccacgc
      601 tgaacccagg cgatgccatt ctgctcggca cgccacaggc gcgcgtggaa atacagccag
      661 gcgatcgcgt gcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg gtagtggacg
      721 aacgtgaagt gaccacgcgc aagagcttcc caacgctgcc acacccgcac ggtacgctgt
      781 ttgccctcgg cctgaactac gccgaccacg ccagcgaact ggaatttaag ccaccggaag
      841 aaccgctggt gttcctgaaa gcgccgaata ccctcactgg cgataaccag acctccgtgc
      901 gcccgaacaa tattgaatac atgcactacg aagcggagct ggtggtagtt attggcaagc
      961 aggcgcgtaa cgtcagcgaa gccgatgcca tgtattatgt cacgggctac accgtgtgta
     1021 acgactacgc cattcgcgac tatctggaaa actactaccg ccctaacctg cgggtaaaaa
     1081 gccgcgacgg actgacgccg atactttcaa ccatcgtgcc gaaagaggcg atcccggacc
     1141 cgcataatct gacccttcgc accttcgtca acggcgagtt acgccagcaa ggcaccaccg
     1201 ccgatctgat cttcagcgtg cccttcctga tcgcctactt aagcgaattt atgaccctga
     1261 atccgggcga catgatcgcc accggcacac caaaaggctt atctgacgtg gtgcctggcg
     1321 atgaagtagt ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt gtgagtgagg
     1381 atacagcgaa atgaaaaaag taaatcattg gatcaacggc aaaaatgtcg caggtaacga
     1441 ctacttccag accaccaatc cggcaacggg tgaagtgctg gcggatgtgg cctctggcgg
     1501 tgaagcggag atcaatcagg cggtagcggc agcgaaagag gcgttcccga aatgggccaa
     1561 tctgccgatg aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga tcgaccagaa
     1621 cgtgcctgag atcgccgcga tggaaaccgc cgacacgggc ctgccaatcc atcagaccaa
     1681 aaatgtgttg atcccacgcg cttcccacaa ctttgaattt ttcgcggaag tctgccagca
     1741 gatgaacggc aagacttatc cggtcgacga caagatgctc aactacacgc tggtgcagcc
     1801 ggtaggcgtt tgtgcactgg tgtcaccgtg gaacgtgccg tttatgaccg ccacctggaa
     1861 ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg aactctcccc
     1921 gctgaccgcc gaccgcctgg gtgagctggc gctggaagct ggtattccgg cgggcgtgct
     1981 gaacgtggta cagggctacg gcgcaaccgc aggcgatgcg ctggtccgtc atcatgacgt
     2041 gcgtgccgtg tcgttcaccg gcggtacggc gaccgggcgc aacatcatga aaaacgccgg
     2101 gctgaaaaaa tactccatgg aactgggcgg taaatcgccg gtgctgattt ttgaagatgc
     2161 tgatattgaa cgcgcgctgg acgccgccct gttcaccatc ttctcgatca acggcgaacg
     2221 ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc tacccggaat tcgttaaacg
     2281 ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga atacccaggt
     2341 tggcgcgctt atcagccagc agcactggga aaaagtctcc ggctatatcc gtctcggcat
     2401 tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc tgcctgcgca
     2461 cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca gacgttgata accgtatgcg
     2521 cgtcgcccag gaagagattt tcgggccggt cgcctgcctg ctgccgttta aagacgaagc
     2581 tgaaggctta cgcctggcaa acgacgtgga gtacggcctc gcgtcgtaca tctggactca
     2641 ggatgtcagc aaagtgctgc gtctggcgcg cgggattgaa gcgggcatgg tgttcgtcaa
     2701 cacccagaac gtgcgtgatt tgcgccaacc gtttggcggc gtaaaagcct ccggcaccgg
     2761 gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg tctgcatttc
     2821 catgggcgac catccaattc cgaaatgggg agtctgatat gggtaagtta gcgttagccg
     2881 ccaagatcac gcacgtaccg tcgatgtatc tctctgaact gccagggaaa aaccacggtt
     2941 gccgccaggg cgcgatcgac gggcataaag agatcagcaa gcgttgccgg gaaatgggcg
     3001 tcgataccat tatcgttttc gatacccact ggctggtcaa cagtgcttat cacatcaact
     3061 gtgcagacca ttttgaaggc atctacacca gcaacgagct gccgcatttt atccgcgata
     3121 tgacctacaa ctacgagggc aacccggagt tggggcagct tattgccgat gaagccttaa
     3181 agctcggcgt gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa tacggcacgc
     3241 tggtaccgat gcgctacatg aatgaagaca agcacttcaa agtggtctcc atttcggctt
     3301 tctgcacggt tcacgatttt gctgacagcc gcaagctggg cgaagcgatt ctgaaagcga
     3361 tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg caccgcttta
     3421 ttgacgatca gcgtgcagaa gaagggatga acagctacac ccgcgagttc gaccgccaga
     3481 tggacgagcg cgtggtgaag ttgtggcgcg aaggccagtt caaagagttc tgcaatatgc
     3541 tgccggagta cgccgactac tgctacggcg aaggcaatat gcacgacacg gtgatgctgc
     3601 tggggatgct cggctgggat aaatacgacg gcaaggtgga gtttatcacc gagctattcc
     3661 caagctctgg caccggtcag gttaacgctg ttttcccgct tcccgcgtaa ggaaccacca
     3721 tgccgcactt tatcgtggaa tgtactgaaa atattcgtga agaagcccgc ttacctgagc
     3781 tgtttgccag tgttaacact gcactggcgg caacggggat ttttccgctt gggggaatac
     3841 gtagccgggc gcactggatc gatacctggc aaatggctga tggcaagcac gattacgcct
     3901 ttgtgcatat gacgctaaaa atcggcagcg ggcgcagcct ggaaagccgt caggaagtgg
     3961 gcgaaatgct gtttgatctg attaaaacgc acttcgcctc actgatggag agccgttacc
     4021 tggcgctttc atttgagatt gcagagctgc acccgacgct gaattttaaa caaaacaacg
     4081 tgcacgcgtt gtttaaataa atttgcacca gcttccaggc cggatgataa ggcgcagccg
     4141 catccggcaa tgccacagga tatcgctatg ttcgataaac acacccacac cctgatcgcc
     4201 cagcgtctgg atcaggcaga aaaacagcgc gaacagatcc gcgcgatctc gctggattac
     4261 ccggagatca ccatcgaaga cgcttacgcg gtgcagcgtg aatgggttcg actgaaaatc
     4321 gccgaaggtc gcacgctgaa aggccacaaa atcggcctga cctcgaaagc gatgcaggcc
     4381 agctcacaaa tcagcgagcc ggattacggt gccctgctgg atgacatgtt cttccacgat
     4441 ggcagcgata tcccgaccga tcgctttatc gtgccgcgca ttgaagtgga gctggctttc
     4501 gtgctggcaa aaccgctgcg tgggccaaac tgcacgctgt tcgacgtcta caacgccacg
     4561 gactatgtga tcccggcgct ggagctgatc gacgctcgct gccacaacat cgatccggaa
     4621 acccagcgcc cgcgtaaagt gttcgacacc atttctgata acgccgccaa tgccggggtg
     4681 atcctcggtg gtcgtcccat taagcccgat gagttggatc tacgttggat ctccgccctg
     4741 atgtatcgca atggcgtgat tgaagaaacc ggcgtcgccg ctggtgtgct gaatcatccg
     4801 gcaaacggcg tggcctggct ggcgaacaaa ctcgcaccgt atgacgtaca actggaagcc
     4861 gggcaaatca ttctcggcgg ttcgttcacc cgcccggttc cggcgcgtaa gggcgacacc
     4921 ttccacgtcg attacggcaa catgggctcc attagctgcc gctttgttta aggagagaac
     4981 gatggaaaac agttttaaag cggcgctgaa agcaggccgc ccgcagatcg gattatggct
     5041 ggggctgagt agcagctaca gcgcagagtt actggccgga gcaggattcg actggttatt
     5101 gatcgacggt gagcacgcgc cgaataacgt gcaaaccgtg ctcacccagc tacaggcgat
     5161 tgcgccctac cccagccagc cggtggtacg tccgtcgtgg aacgatccgg tgcaaatcaa
     5221 acaactgctg gacgtcggca cacaaacctt gctggtgccg atggtacaaa acgctgacga
     5281 agcccgtgaa gcggtacgcg ccacccgtta tccccccgcc ggtattcgcg gtgtgggcag
     5341 tgcgctggcc cgcgcctcgc gctggaatcg tattcctgat tacctgcaaa aagccaacga
     5401 tcaaatgtgc gtgctggtgc agatcgaaac gcgtgaggca atgaagaact taccgcagat
     5461 tctggacgtg gaaggcgtcg acggcgtgtt tatcggcccg gcggatctga gcgccgatat
     5521 gggttatgcc ggtaatccgc agcacccgga agtacaggcc gccattgagc aggcgatcgt
     5581 gcagatccgt gaatcgggca aagcgccggg gatcctgatc gccaatgagc aactggcaaa
     5641 acgctatctg gaactgggcg cgctgtttgt cgccgtcggc gttgacacca ccctgctcgc
     5701 ccgcgccgct gaagcgctgg cagcacggtt tggtgcgcag gccaccgccg tgaagcccgg
     5761 cgtgtattaa tgccggatgc gctgcgcatc cgccataaac cttgtcgtac cctacaaaaa
     5821 tcccattaga ggaagaaaaa tgagcgacac ctcacctgcc ataccggaga gtatcgatcc
     5881 ggcgaatcag cataaagcgc tgactgccgg acaacaggcg gttattaaga agctatttcg
     5941 ccgcctgatc gtctttctgt tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat
     6001 cggctttgcc ggactcacga tgggacgcga cctcggtctg agcgctacca tgtttggcct
     6061 cgctaccacc ctgttctatg ccgcttatgt catcttcggc attcccagca acattatgct
     6121 gagtattgtc ggtgcgcggc gctggatcgc caccatcatg gtgctctggg gcatcgcctc
     6181 tactgccacc atgtttgcca ctggccccac cagcttgtac gtactgcgta tactggttgg
     6241 cattaccgaa gccggctttc tgcctggtat tctgctgtat ttaaccttct ggtttccggc
     6301 ctatttccgc gcccgtgcca acgccttgtt tatggtagca atgccggtaa cgacagcgtt
     6361 gggctcgatc gtttccggct acattttgtc gctggatggc gtcatggcat taaaaggctg
     6421 gcagtggctg tttttgctgg aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt
     6481 ctggcttgat gactcaccgg acaaagctaa gtggctgacg aaagaagaca aaaaatgcct
     6541 gcaagagatg atggataacg atcgtctgac gctggttcag ccagagggag ccatcagcca
     6601 ccacgccatg caacaacgca gtatgtggcg ggagatcttc actccggtgg tgatgatgta
     6661 taccctggcg tatttctgcc tgaccaacac acttagtgcg atcagcatct ggacaccgca
     6721 gatcctgcaa agttttaatc agggcagcag taatatcacc atcggcctgc tggccgccgt
     6781 accgcagatt tgtaccattc tcgggatggt ctactggagc cgtcactcag atcgccgcca
     6841 ggaacgaagg catcacaccg cccttcctta tttgttcgct gccgcaggtt ggttactggc
     6901 ttcggcaact gatcacaaca tgatccagat gctggggatc attatggctt cgaccggatc
     6961 attcagcgca atggcgattt tctggacaac accggatcag tccatcagcc tgcgggcacg
     7021 agcgatcggt attgcggtga tcaacgccac tggcaacatt ggttcagcgt taagtccgtt
     7081 tatgatcggc tggttgaaag atttgaccgg cagctttaac agtggattgt ggtttgttgc
     7141 cgcgctgctg gtgattggtg cggggattat ctgggcgatt ccaatgcagt cctcccgtcc
     7201 gcgagcgacc ccgtaaggaa cgacgatgtg tgaccgtcag attgccaata ttgatatcag
     7261 caaagagtac gatgaaagcc tgggcacgga cgatgtgcat tatcagtcgt tcgcccgcat
     7321 ggcggccttt tttggccgcc atatgctgcc acatcgccac gaacagtact ttcagatgca
     7381 tttcctcaat agcggacaga ttgagctaca acttgacgat catcgctact cggtggaagc
     7441 gcccctgttt gtcctgacgc cgccgtcagt acctcatgcg tttattacgg agtctgacgc
     7501 cgacggtcat gtgttgacgg tacgggaaga tctgatctgg cccctgctgg aagttcttta
     7561 tccgggcact cgggaaacct tcggcctgcc ggggatttgc ctgtcactgg cagataaacc
     7621 cgacgaactg gcggcgctgg aacactattg gcaactgata gagcgggaat cggtagaaca
     7681 actgcctgga cgggaacaca ccctgacgtt actggcacag gcagtattca ccctactgct
     7741 gcgtaatgca aaactcgacg accatgccgc cagcggaatg cgcggagaat taaaactgtt
     7801 ccagcgtttt aatatgctga ttgaaagcca ttttcatcag cactggacag taccggatta
     7861 cgctaacgaa ctgcatatca ccgaatcacg cctcacggac atctgccgcc gctttgccaa
     7921 ccgtccacca aaacggttga ttttcgacag gcagctgcga gaagccaaac ggctgctgct
     7981 gttttctgat aacgcagtga acaatattgc ctggcaactc ggttttaaag atccggctta
     8041 ttttgcccgc ttttttaatc gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa
     8101 agtacctgtg acgtgaaaat cccttagcct taacgggaaa ccaggcacca cctgctattc
     8161 ccctttctcc gcctggagca aggggattta gtaacgtcaa aaatcgctaa aagcgacctc
     8221 gatcacaaaa ccgtacccga cctgaaaagt accagccatt gtcccaaaag tctcttcccg
     8281 taatcgcccc tgcaagcttc aattgaataa caaaaacaac acacaaaatt aacaatcgta
     8341 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
     8401 gtccgttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
     8461 atggcgagcg agtgaaagac gtcactactc atccggcatt tcgtaatgcg gcagcgtctg
     8521 ttgcccaact gtacgacgcg ctacacaaac cggagatgca ggactctctg tgctggaaca
     8581 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
     8641 acctgcgcca gcaacgcgat gccatcgctg agtggtcacg cctgagctat ggctggatgg
     8701 gccgtacccc agactacaaa gctgctttcg gttgcgcact gggcgcgaat ccgggctttt
     8761 acggtcagtt cgagcagaac gcccgtaact ggtacacccg tattcaggaa actggcctct
     8821 actttaacca cgcgattgtt aacccaccga tcgatcgtca tttgccgacc gataaagtaa
     8881 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     8941 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     9001 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     9061 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     9121 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     9181 tgctgatccc atgggaaaac gtactgatct accgcgattt cgatcgctgc cgtcgctgga
     9241 caatggaagg cggttttgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     9301 aactcgactt cattacagca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     9361 tccgtggtgt acaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     9421 tgagtgactc gatgtgttct gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     9481 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     9541 ttatcgaacg caacgttacc agtggcctga tctatctccc ttccagtgcc cgtgacctga
     9601 acaatccgca gctcgaccag tatctggcga agtatgtgcg cggttcgaat ggtatggatc
     9661 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gagtttggtg
     9721 gtcgccacga actgtatgaa atcaactact ccggtagcca ggatgagatt cgcctacagt
     9781 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     9841 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cttgcacaac aacgacgata
     9901 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     9961 acagcgcctg cgctttcgtg acgcgatggc cagcctgtcg gcagcggtaa atattatcac
    10021 caccgagggc gacgccggac aatgcgggat tacggcaacg gccgtctgct cggtcacgga
    10081 tacaccacca tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
    10141 gggcaacggt aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcacgcca
    10201 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
    10261 aggtccgctg gcgcagccgg tgctgaaagg ttcgctggcc agtcttgaag gtgagatccg
    10321 cgatgtgcag gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
    10381 cagtgcagaa ggtcacggac ttatctactt taaacgccgt ttccatccgg tgatgctgga
    10441 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
    10501 tgtgatctca atcacatatc cacccgatga ataattcttg cccaaccgct caccgccatt
    10561 cctgaccgct tacgtcccta aaataaccac tcagttattt accttacttt acgcgcgcgt
    10621 aactctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
    10681 ctagaacggc ttcggcctat gattaacaaa ttactctatg aattttggtt tgcaggaagg
    10741 cagcaagtga gtgaatcccc gggagcttac aacagtaagt gacaggggtg aacgaacgca
    10801 actgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
    10861 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
    10921 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
    10981 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
    11041 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
    11101 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
    11161 ctggtcggtc cggttctcgc cgcacagatg ggctacctgc ctggcacgct gtggctgctg
    11221 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
    11281 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
    11341 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc ttgccgtcct ggcgctgatt
    11401 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
    11461 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
    11521 tctgtcattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
    11581 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
    11641 ggctatgcgt ttgtttccgc attgctgccg gtgtggctga tcctcgcacc gcgcgactat
    11701 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
    11761 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
    11821 aaaggtgctc tgttcccgtt cttgttcatt accatcgcct gtggtgcggt atctggcttc
    11881 cacgcactga tctcttccgg tacgacgccg aaactgctgg ctaatgaaac cgacgcgcgt
    11941 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgct
    12001 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
    12061 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
    12121 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
    12181 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
    12241 ggtggtgcgc caacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
    12301 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
    12361 gcgctggatg cgggtactcg ttctggccgc tttatgctgc aagacctgct gggtaacttc
    12421 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
    12481 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
    12541 agcctgtggc cgctgttcgg tatttccaac cagatgctgg cagccgtagc gctggtactg
    12601 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
    12661 gctgtatggc tgcttatctg caccacctgg gcgctgggcc tgaaactgtt cagcaccaac
    12721 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
    12781 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtaaa caactacacc
    12841 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
    12901 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac tccgtacgtt
    12961 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
    13021 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
    13081 atatctcggc caggcggcga agatgttgat tggcattcca gactatgaca actacgtcga
    13141 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
    13201 acgccagaac gcacgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
    13261 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacca
    13321 ccctgctgcg ccatattctt aatgaacaac acggctacaa gattgccgtg attgaaaacg
    13381 aattcggcga agtctccgtt gatgatcaat tgattggtga tcgcgcaacg cagatcaaaa
    13441 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcgctactcg
    13501 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtc attgaatgca
    13561 ccggcatggc cgatcctggc ccgattattc aaaccttttt ctcccatgag attttatgcc
    13621 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
    13681 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
    13741 aaaccgacgt cgcaggcgaa gcagaaaaac tgcgtgaacg tctggcacgc atcaacgccc
    13801 gtgcaccggt ctacaccgtc acccacggcg acatcgacct gggtctgctg ttcaacacca
    13861 acggttttat gctggaagaa aacgtcgtca gcaccaaacc gcgtttccat tttatcgcgg
    13921 acaaacaaaa cgatatttcg tcgattgtgg tggaactgga ttacccggta gatatcagcg
    13981 aagtttcccg ggtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    14041 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    14101 tctacagcgc cgactgggac aggccatggg gcgatgaaaa accgcatagc acgatggtgt
    14161 ttatcgggat tcagttgccg gaggatgaga ttcgggcggc gtttgcggga ctcaaaaagt
    14221 aaccagccct ccaacatcac aatacctgag actttatcac cctcatattc ctccagaata
    14281 tgagggcatt cgggcttaat tatctctgaa cgtgcaacac ttcctgcgac tccttatgcg
    14341 cctccacatc ctgcttcgtt aaccagagcg acttacgacc aaaattttcg tacattttca
    14401 gctgatcttc atagtgctta tcaactgttc catcgggagc aataaaccca ctctgaccgg
    14461 gtgcgaccac atcccaggca agcacaggac gatcgcttgt cgttggtgag aaaacaatca
    14521 tatcgttttc tgttccacgg ttttgatact ccgcctgatg acgcgtttct tccgctgcgg
    14581 cctgcggtac accaaagaaa ttatttgccc ggaacgttaa ggccattgca ggtgttttcc
    14641 agttactcac attattgcca tagcgtttgg aaagagtctc ccaggtatct tccagcgcag
    14701 ccaacacaac ctcctgctgt ggtttcccag caaacagatc aaccgcctgt gggattggtg
    14761 atttgtctcc ctgcaccgcc tcatacaaaa tttttgctcc aacacttata ttcagcgaac
    14821 cagttgggcc gtcctgggtt gtttcgtagc cactggcgct gtaccactta tcaaatggca
    14881 taggtacggc agccactacg gtacgcttca acatactggt cagccaaacg ttcaggatgg
    14941 cagagcctgg ctgctgccag gttttaccat catcattaag caaattgatg ccatcccaac
    15001 gtgttaatgt atccaccaac tgacggcgcg gatcgctctg tgtcaaacca gatgtcgctg
    15061 cttgcagagt aggtaaaaaa agcctcaggt taagatcctg acgactggtt tggcgaataa
    15121 catcccatgc ctgatcagca gttaagcgtg gcttttgctc aagcagtcgg tcgatctccg
    15181 taacgcgatc tgcccccccc cacaaaaagg caaacagatc cgaagcggga taatcttttt
    15241 ggggagaatt gttccagtta gcaatatatc ccgacagggg gttatacacc ttagggttca
    15301 tttcaaaagg caatagccct ttccagtccc attttcccgt accaggaacg ggtaatcgcg
    15361 gatcatggcc tgattgacga tctggataag caccagtatg aacataacca atattgccgt
    15421 ttacatcagc atagtaccag ttgatggtca gtgcttgttt cgctgcctgc tgtgtccact
    15481 cctgccaatt tttggccttc atctgatgag tccaggccag caaagacgcc acctctttac
    15541 catcccatgc gcgggattta gcgtaagccg tttgtgtcgt ctggtcagtt tggagaatgt
    15601 tgccatgcac cgtacgccag acagtaaagg tctctgcctg accatttttc accgtaatgg
    15661 tttcctcacg gcttaacatt ttcacccact taccattatg caagtagtag cctggtttct
    15721 ctgccgacag ccgttcagca aaaatatcga catcatcgcc gaaacctgcc gttgatcccc
    15781 aggaaatcac accattatga ccaaaaacca gcccaggata ggcaaatggt gtattgccag
    15841 tgacatcata accagcaccg tgcagaccaa taccataagt atacgcaggc gcataccagc
    15901 caaactgcgg accatttacc atgattgctt tcgcatcctg ggctttgctt ttgccgatca
    15961 cccacatatt gctggtcgtt ggataccccg ccagaccatt ggcaccaccc tgtgcaaatt
    16021 gtgcagcaat agtttcccgg ttcttccctg ctgttaacgc cagcagtgcg ccatccgccc
    16081 cttttgctgg tcggtcaagc attggtgcag gtaaatcgta gcgtggcaac agagctgctg
    16141 tttgcgagtt ttgctgatta aatttaagtg ggtagctact ctcttgtacg gcaatagtgg
    16201 ttggcgctga tgggtttacc agccatttca a
//
LOCUS       DAZUFB010000110        16075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZUFB010000110 DAZUFB010000000
VERSION     DAZUFB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.110"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHY3M_003943"
     CDS             complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHY3M_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFS4318767.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
     gene            188..682
                     /gene="napF"
                     /locus_tag="ACHY3M_003944"
     CDS             188..682
                     /gene="napF"
                     /locus_tag="ACHY3M_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFS4318768.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            672..935
                     /gene="napD"
                     /locus_tag="ACHY3M_003945"
     CDS             672..935
                     /gene="napD"
                     /locus_tag="ACHY3M_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFS4318769.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            932..3418
                     /gene="napA"
                     /locus_tag="ACHY3M_003946"
     CDS             932..3418
                     /gene="napA"
                     /locus_tag="ACHY3M_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFS4318770.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3425..4120
                     /gene="napG"
                     /locus_tag="ACHY3M_003947"
     CDS             3425..4120
                     /gene="napG"
                     /locus_tag="ACHY3M_003947"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFS4318771.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4107..4970
                     /gene="napH"
                     /locus_tag="ACHY3M_003948"
     CDS             4107..4970
                     /gene="napH"
                     /locus_tag="ACHY3M_003948"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFS4318772.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4967..5416
                     /gene="napB"
                     /locus_tag="ACHY3M_003949"
     CDS             4967..5416
                     /gene="napB"
                     /locus_tag="ACHY3M_003949"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFS4318773.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGALRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5426..6028
                     /gene="napC"
                     /locus_tag="ACHY3M_003950"
     CDS             5426..6028
                     /gene="napC"
                     /locus_tag="ACHY3M_003950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFS4318774.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHY3M_003951"
     CDS             6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHY3M_003951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFS4318775.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHY3M_003952"
     CDS             6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHY3M_003952"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFS4318776.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGAL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHY3M_003953"
     CDS             7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHY3M_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFS4318777.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHY3M_003954"
     CDS             8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHY3M_003954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFS4318778.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHY3M_003955"
     CDS             8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHY3M_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFS4318779.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHY3M_003956"
     CDS             8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHY3M_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFS4318780.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHY3M_003957"
     CDS             10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHY3M_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFS4318781.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHY3M_003958"
     CDS             11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHY3M_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFS4318782.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHY3M_003959"
     CDS             complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHY3M_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFS4318783.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13329..15919
                     /gene="yejO"
                     /locus_tag="ACHY3M_003960"
                     /pseudo
     CDS             13329..15919
                     /gene="yejO"
                     /locus_tag="ACHY3M_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3576 a         3943 c         4682 g         3874 t
ORIGIN      
        1 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
       61 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
      121 cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
      181 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
      241 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
      301 ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
      361 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc
      421 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
      481 tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
      541 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
      601 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
      661 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
      721 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
      781 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
      841 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
      901 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
      961 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
     1021 cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc gtttctgcgg
     1081 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
     1141 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
     1201 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
     1261 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
     1321 attcaaaacc gccctgaaag aaaaaggacc ggaatcgatc ggtatgttcg gttctggtca
     1381 gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct tccgttcgaa
     1441 caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
     1501 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
     1561 gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca tcactaaccg
     1621 tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
     1681 cgagctggcg gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta
     1741 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaagcacgt
     1801 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
     1861 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
     1921 agccttcgtt gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga
     1981 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
     2041 gacgatgggc ttcaaccagc atactcgtgg cgtgtgggcc aacaacctgg tctacaacct
     2101 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
     2161 gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
     2221 catggtggtg actaacgaga aacaccgtga tatttgcgag aagaagtgga atatcccgag
     2281 cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cactgaaaga
     2341 cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
     2401 taatgaagag cgtatgccgg gctggcgtga tccgcgcaac ttcatcatcg tctccgatcc
     2461 gtatccgaca gtcagtgcgc tggcagccga cttgatcctg ccgaccgcaa tgtgggtaga
     2521 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaggtaca
     2581 ggcgccaggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
     2641 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
     2701 gctgtacgaa gttctgtatg ccacccccga agtgagcaaa taccctgtat ccgaactggc
     2761 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
     2821 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
     2881 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
     2941 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggtaaacc
     3001 ggatggcaaa gcggtgatct tcgcactgcc gttcgaaccg gcggcggaag caccggatga
     3061 agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat
     3121 gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc
     3181 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
     3241 tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt
     3301 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg atgcgaccga
     3361 tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc
     3421 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt
     3481 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
     3541 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
     3601 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
     3661 gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
     3721 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga
     3781 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
     3841 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
     3901 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
     3961 gacggttcac agcgacgcct gtaccggttg cggtaaatgc gaaaaagtat gcgtgctgga
     4021 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gtcaccatta
     4081 ccgcttcggt tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
     4141 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
     4201 agttcttcgt gctggggatg tttttgagcg gtccatggtt tggtgtgtgg atcttgcacg
     4261 gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
     4321 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
     4381 tcaccgtgct ctacgccctg gcgggaaagc gattattttg cagctgggtc tgcccgctga
     4441 acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
     4501 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa
     4561 ctggcacgct catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa
     4621 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
     4681 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
     4741 gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt
     4801 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
     4861 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
     4921 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
     4981 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
     5041 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg
     5101 ggccattcgg atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc
     5161 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
     5221 gtgccacggt gtcgaaagtt atcgcaccac tggcgcgctg cgtatcagtc ctactcactt
     5281 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
     5341 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
     5401 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
     5461 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
     5521 cggttttgtt ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa
     5581 taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat
     5641 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
     5701 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
     5761 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
     5821 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
     5881 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
     5941 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga
     6001 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
     6061 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
     6121 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
     6181 tttgctgacg gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt
     6241 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
     6301 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc
     6361 acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa
     6421 tcagctctcg gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
     6481 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
     6541 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
     6601 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc
     6661 atgatgttct ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa
     6721 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
     6781 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt
     6841 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
     6901 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
     6961 tggatggtaa gcggtctgcc gttactcatt ctttcgccac tggtagcaat gctactggga
     7021 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
     7081 ggctttctcg gtgcaccggg cgtggcgctg acagtgggac ttaagcgcgg tggtgcgctg
     7141 ctcagcatac tggtgttacc gctgactatc ccattactca tcttcgccac cgccgcgatg
     7201 gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
     7261 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
     7321 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
     7381 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
     7441 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
     7501 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
     7561 ggtcgatggg catttatgca tcaatggcag tggcagcgtt tattggcctt gtctggcaga
     7621 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
     7681 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
     7741 atgcacgtct gacttctgaa ctggtgctgc tttttttgta tgtgggtgtg attgccctgt
     7801 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
     7861 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
     7921 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
     7981 tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
     8041 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
     8101 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
     8161 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
     8221 gcagcatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg
     8281 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
     8341 tgtgctgttc tggcagggtt ggcactgact atcggtctgg tgctatatgc gctgcgctcg
     8401 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa
     8461 atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagcgtgcag
     8521 cgcgatccca attcgctaaa agtaactttc accatttacg acgctgaagg ttcagtagat
     8581 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
     8641 ggcgaactgg aaaaaggcaa tcatatcctc gcaaaagaag tgctggcgaa acacgacgaa
     8701 aactacacgc cgccagaagt tgagaaagcg atggaagcca atcaccgtcg cccggcgagt
     8761 gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg tgtctggcgc
     8821 taggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtagcgcgc ggagatgcgc
     8881 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat
     8941 ttctggtact ggtcaatgcc ttcgtggtca acgacttcac cgtcacctat gttgccagca
     9001 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag
     9061 gctcgctcct gctgtgggtg ctgctgatga gcggctggac ctttgcggta gcgattttta
     9121 gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg atggtcagcg
     9181 tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ttgccgaact
     9241 tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
     9301 cgcctctgct ctatatgggg tacgtggggt tctcggtggc gtttgctttt gccattgctt
     9361 ctttgttgtg cgggcgtctg gacagcactt atgcgcgttt tactcgtccg tggacgctgg
     9421 cggcgtggat cttcttgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
     9481 tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
     9541 tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
     9601 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg
     9661 tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt
     9721 ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg
     9781 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag
     9841 cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc
     9901 tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
     9961 tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct
    10021 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc
    10081 tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    10141 tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta
    10201 tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct cacctggggc
    10261 tggcagtgac aattgttggt attgccttta gccagaacta tagcgttgag cgtgatgtgc
    10321 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca
    10381 aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac tatcggcgta acgcgcgatg
    10441 gcaagccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    10501 tgaccgaagc ggcaattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    10561 agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accatttgtt cgctggattt
    10621 gggcgggcgg gctgatgatg gcgttgggcg gactactgtg tctgtttgat cctcgctatc
    10681 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggccgt atgaagcgca aagtattgtt
    10741 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa
    10801 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc ctgtgccgaa
    10861 gtttcgtctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    10921 gggcaaacca gtactgctta acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca
    10981 tcaatatctg aatcagcttt cagcgcaggg cattcgcgtg gtcggcatga actataaaga
    11041 cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg cgctaagcct
    11101 gtttgatggc gacggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacgtt
    11161 ccttattgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    11221 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    11281 tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg
    11341 ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga agaactgcgc
    11401 tgcccgaaat gccagaataa cagcattgcc gattccaact cgatgattgc caccgacctg
    11461 cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta agaaagagat tgtcgattat
    11521 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    11581 ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat ttacgcccgc
    11641 tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg aacaaagcgt gccggaaggt
    11701 aagcgtgccg gatatgtcgc ttatttgccg ggcattgtgg tggcgttaat tgtggctggc
    11761 gtcagctact accagaccgg aaattatcaa caggtgaaaa tctggcagca ggccacggca
    11821 caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa
    11881 gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    11941 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgctag tatcgccacc
    12001 gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gcgatgccgc actgggttat
    12061 gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggcgg cgaactgctg
    12121 cgccagttgg tgagaagcga ccacagcaat atccgtgtgt taagcatgta tgcgtttaat
    12181 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    12241 ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa
    12301 catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctatt
    12361 tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat
    12421 tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca
    12481 ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca tccagctcgc
    12541 gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt
    12601 actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca
    12661 gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagcg
    12721 caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat
    12781 ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca
    12841 agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt
    12901 cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg cgtcgcataa
    12961 gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata gtagtctcct
    13021 gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga
    13081 atgttacccc attttatgta tggacatgta gtgaaaacta caaccttgtc gttcgctttg
    13141 gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga aaaacaacta
    13201 aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact gactcccggt
    13261 atggagctat gggtattttc tgtacccaat gcttttaaca gcaattaatt tcataggatg
    13321 aaagctcaat gcatcaatct ggttctgttt ctctttgtcg ttccgcaata tctgttctgg
    13381 tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat ggtgagacag
    13441 ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc aataatacga
    13501 aaatcaatcc tggcggagaa cagcatataa aagaatttgg tgtaagtaat aatactgaaa
    13561 ttaacggagg gtatcagtac attgaaatga atggcgccgc agaatactca gtattaaatg
    13621 acggttatca aattgttcaa atgggtggcg cggcaaacca gactacgctc aataatggtg
    13681 tgctacaggt ttatggcgca gcgaatgata ccacgattaa aggcgggcgc ttaatcgttg
    13741 aaaaagatgg gggggccgtc tttgtcgcta tcgaaaaggg aggactactg gaggttaaag
    13801 aggggggatt agcgattgcg gtagatcaga aagcaggcgg tgctattaaa acaaccacgc
    13861 gggccatgga ggtattcgga acaaaccgtc tcggtcagtt cgatatcaag aatggtattg
    13921 ctaataatat gttgttggaa aacggcggaa gtttgcgagt tgaagaaaat gacttcgctt
    13981 ataataccac tgtagatagt ggcggcttac tggaggttat ggatggcggg actgtaactg
    14041 gcgttgataa aaaagcaggc ggaaaattaa ttgtctcaac gaatgcgctg gaagtgagtg
    14101 gtccaaacag taaaggccaa tttagtataa aagatggtgt gtcaaaaaat tatgaactgg
    14161 atgatggttc cgggctcatt gttatggagg acacgcaggc cattgatact atccttgatg
    14221 agcatgccac tatgcaatcg ctgggaaagg atactggtac gaaagtgcag gcaaatgcgg
    14281 tatatgatct cggtcgatca tatcagaatg gaagtatcac gtattcctca aaagccatct
    14341 ctgaaaatat ggttatcaac aatggccgcg ctaacgtctg ggctggcaca atggttaacg
    14401 tttcagtcag agggaatgat ggcattcttg aggtcatgaa gccgcaaata aattatgcac
    14461 ccgcaatgtt ggtgggtaag gtagtggttt ctgagggcgc ttcttttaga acgcatagtg
    14521 ccgtggatac cagcaaagcg gacgtttcgc tcgaaaatag cgtatggacc atcattgccg
    14581 atatcactac gacgaaccaa aacaccctcc tcaacttagc caaccttgcg atgtctgacg
    14641 caaatgtgat tatgatggat gagccagtga ctcgttcatc agtgacggca agtgcggaaa
    14701 atttcattac gttgaccacc aataccctgt cgggaaacgg caatttttat atgcgtaccg
    14761 atatggctaa tcatcagagc gatcagctca acgtcaccgg tcaggcaaca ggtgatttca
    14821 aaatattcgt gacggacacc ggtgccagcc cggcagcagg agatagcctt acactggtaa
    14881 caacgggcgg cggtgatgct gcatttacgt tgggcaatgc cggaggcgtt gttgatatcg
    14941 gtacgtatga atataccttg ctggataatg gcaaccatag ctggagtttg gcagagaatc
    15001 gcgcgcaaat taccccttca accactgatg tgctgaatat ggcggcagca caaccgctgg
    15061 tatttgatgc agaactggac accgtgcgtg agcgtcttgg tagcgtaaaa ggcgttaatt
    15121 acgatacggc gatgtggagt tcggcaatta acacccgcaa caacgtgacc actgatgcgg
    15181 gcgctggctt tgagcaaaca ttgaccgggc tgacgctcgg tatcgatagc cgtttctccc
    15241 gtgaagaaag cagcacaatt cgcggcttgt tctttggtta ctctcattct gatattggtt
    15301 ttgatcgcgg cggcaaaggc aatgtcgata gctataccct gggggcttat gccggttggg
    15361 agcatcagaa cggtgcctat gttgatggag tggtgaaagt tgaccgtttt gccaacacca
    15421 tccatggcaa gatgagtaat ggggcaacag cgtttggcga ttacaatagt aacggcgcgg
    15481 gtgctcatgt cgagagcggg ttccgttggg ttgacggatt gtggagtgtt agaccctatc
    15541 tggcctttac cggctttacc acagatggtc aggactacac gttatcaaac ggcatgccgc
    15601 ggatgtggga aatacccgga tattacgcgc tgaagcggga acggcggtaa gctatcacat
    15661 ggacctgcaa aacggtacga cgctggaacc ctgactgaaa gccgccgtgc gtcaggaata
    15721 cgccgattct aaccaggtga aagttaatga cgatggcaaa tttaataatg atgtggctgg
    15781 aacccgtggc gtttatcagg ctgggataag gtcatcgttt accccgacgt taagcggtca
    15841 tttgtcagtc agctatggca atggcgcagg ggtagaatcg ccgtggaata ctcaggcggg
    15901 tgtggtctgg acgttctgat aacaaaaaat aaacaggctg tgatgtgtca cggtctgttt
    15961 atcgaattaa ttgcagatat aaaaaaaacc aaccgtaaag gttggttttt tcttgggatt
    16021 tttggcacga gagtagttta attgagtatg taactacatg attttaaata atatg
//
LOCUS       DAZUFB010000111        16074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZUFB010000111 DAZUFB010000000
VERSION     DAZUFB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.111"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHY3M_003961"
     CDS             complement(<1..275)
                     /locus_tag="ACHY3M_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS4318784.1"
                     /translation="MSEYVELAYAAATSRLCLFTGTGFSKAVSNGEAPTWKELLKKVC
                     SKVHNAKQLEDTLFPDDKPPALSLEEAAQILSLKLNIEGKSIYDEIV"
     gene            complement(332..973)
                     /locus_tag="ACHY3M_003962"
     CDS             complement(332..973)
                     /locus_tag="ACHY3M_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HFS4318785.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLSPLINIIRGTQTLAQKRVPKGELTGKGNYL"
     gene            complement(1162..1722)
                     /locus_tag="ACHY3M_003963"
     CDS             complement(1162..1722)
                     /locus_tag="ACHY3M_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HFS4318786.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISNEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            complement(1968..2279)
                     /locus_tag="ACHY3M_003964"
     CDS             complement(1968..2279)
                     /locus_tag="ACHY3M_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HFS4318787.1"
                     /translation="MRKDFKIDGKYVVLSVSSQIQSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMAEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(2330..3361)
                     /locus_tag="ACHY3M_003965"
     CDS             complement(2330..3361)
                     /locus_tag="ACHY3M_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4318788.1"
                     /translation="MTNSLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSDHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(3358..3753)
                     /locus_tag="ACHY3M_003966"
     CDS             complement(3358..3753)
                     /locus_tag="ACHY3M_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318789.1"
                     /translation="MFNGLTDAKVNALLNDAKQKAANPGTNENERAMAVAIWELAEEV
                     KRNRLLYTRVYQQFDGASWIDIEEAEYATCKKEKKIVRILHKAATPITVEIPDHRYFE
                     NDDLPGDVIRIIDEILHEQGLTVKITHNK"
     gene            complement(3773..3994)
                     /locus_tag="ACHY3M_003967"
     CDS             complement(3773..3994)
                     /locus_tag="ACHY3M_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HFS4318790.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKWFERDSYQLSLSRGEEKIER
                     TRGRWRFYDENHNQVKAEPILYTLLKTII"
     gene            4599..4808
                     /locus_tag="ACHY3M_003968"
     CDS             4599..4808
                     /locus_tag="ACHY3M_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HFS4318791.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            4919..5770
                     /locus_tag="ACHY3M_003969"
     CDS             4919..5770
                     /locus_tag="ACHY3M_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HFS4318792.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(5803..6918)
                     /locus_tag="ACHY3M_003970"
     CDS             complement(5803..6918)
                     /locus_tag="ACHY3M_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFS4318793.1"
                     /translation="MRTSLVTREEMIEAIEQHTSCISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAETRKEAHRMKATNVKSEKTHAPSVQES
                     ELVVVQNQSDEIPVLEWLGVRVVTTKILAKGYGTDADNIKKNFSRNAERFCEGKHFYK
                     VIGDDLNNLRVTLSNSQNPISPKARSLILWTERGAARHAKMIETDQAWAFFEKLEDSY
                     FRQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSV
                     AKGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLD
                     DEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(6915..7136)
                     /locus_tag="ACHY3M_003971"
     CDS             complement(6915..7136)
                     /locus_tag="ACHY3M_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4318794.1"
                     /translation="MATVPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(7155..7274)
                     /locus_tag="ACHY3M_003972"
     CDS             complement(7155..7274)
                     /locus_tag="ACHY3M_003972"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFS4318795.1"
                     /translation="MVVLAGLPKGRPVPFDAGSLNPVSATAIEIETSRGSSLT"
     gene            complement(7496..8980)
                     /locus_tag="ACHY3M_003973"
     CDS             complement(7496..8980)
                     /locus_tag="ACHY3M_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS4318796.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(8980..10173)
                     /locus_tag="ACHY3M_003974"
     CDS             complement(8980..10173)
                     /locus_tag="ACHY3M_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS4318797.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEENQTASGAPSLEDLDKIARERAA
                     SRRADAALWIEQRREEIADIVDTGGYGDVDNEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(10259..10681)
                     /locus_tag="ACHY3M_003975"
     CDS             complement(10259..10681)
                     /locus_tag="ACHY3M_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HFS4318798.1"
                     /translation="MEINHSRITSYEIADYMIRTKSLLSAKELAAILEKEYPHLDVDK
                     RDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRFDEKLQNELYM
                     TQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(10800..11843)
                     /locus_tag="ACHY3M_003976"
     CDS             complement(10800..11843)
                     /locus_tag="ACHY3M_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4318799.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAAPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GARITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            complement(11871..12050)
                     /locus_tag="ACHY3M_003977"
     CDS             complement(11871..12050)
                     /locus_tag="ACHY3M_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFS4318800.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIRARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(12055..12435)
                     /locus_tag="ACHY3M_003978"
     CDS             complement(12055..12435)
                     /locus_tag="ACHY3M_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4318801.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(12435..12656)
                     /locus_tag="ACHY3M_003979"
     CDS             complement(12435..12656)
                     /locus_tag="ACHY3M_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4318802.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(12729..13118)
                     /locus_tag="ACHY3M_003980"
     CDS             complement(12729..13118)
                     /locus_tag="ACHY3M_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4318803.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            complement(13240..13518)
                     /locus_tag="ACHY3M_003981"
     CDS             complement(13240..13518)
                     /locus_tag="ACHY3M_003981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318804.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            complement(13791..14471)
                     /locus_tag="ACHY3M_003982"
     CDS             complement(13791..14471)
                     /locus_tag="ACHY3M_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318805.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTRQLRERISRV
                     LGDVITPDHHKQAEKSDLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVAEKH
                     EADHANES"
     gene            complement(14453..14827)
                     /locus_tag="ACHY3M_003983"
     CDS             complement(14453..14827)
                     /locus_tag="ACHY3M_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318806.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLLNS
                     LRILPQTEVGELHLWLLCFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRRKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            complement(14834..15127)
                     /locus_tag="ACHY3M_003984"
     CDS             complement(14834..15127)
                     /locus_tag="ACHY3M_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318807.1"
                     /translation="MWRGNSHGKSQMILTEYQFDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            complement(15306..15539)
                     /locus_tag="ACHY3M_003985"
     CDS             complement(15306..15539)
                     /locus_tag="ACHY3M_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318808.1"
                     /translation="MGKITFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKMELLTH"
     gene            complement(15625..15885)
                     /locus_tag="ACHY3M_003986"
     CDS             complement(15625..15885)
                     /locus_tag="ACHY3M_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HFS4318809.1"
                     /translation="MMDVKEKVLQVMRSRAALQDKALGGEYPFRMATWNLRLAMEKEF
                     PDEEWRSADLRKILMEMAKDGTASKDTHASRIGQAVWRLEVR"
     gene            complement(15882..>16074)
                     /locus_tag="ACHY3M_003987"
     CDS             complement(15882..>16074)
                     /locus_tag="ACHY3M_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4318810.1"
                     /translation="DGWISCSERMPPQDDWILIYSKHGEYMAGQVQGEYVELSDGTLS
                     WLGNVLFWMPLPEPPKGAK"
BASE COUNT         4055 a         4124 c         3473 g         4422 t
ORIGIN      
        1 tcaacaattt catcataaat cgacttgcct tcaatgttga gcttcaaaga tagtatctgt
       61 gcggcttctt caagactcag tgctggtggt ttatcatcag ggaataacgt gtcttccaac
      121 tgtttggcgt tgtgaacttt gctgcaaact ttttttaata gttctttcca cgttggtgct
      181 tcgccgttcg agactgcctt tgaaaagcca gtcccggtaa atagacaaag cctgcttgta
      241 gcggcagcgt atgctaactc aacatattcg ctcattactt actccattta cttatagttc
      301 tatggagaag tataccctaa tgcattgacg tttatagata atttcctttt cctgttaatt
      361 cccctttcgg cacccgtttt tgtgccagcg tttgggttcc gcgaattatg ttaatcagag
      421 ggcttagtaa cgatggctcc tggcgggcct caacttctcc agccattgcc ctgatgtagt
      481 cggcgctggc aacgttgttg tattccgtcg caaagcaaca tagtaacgtc agaacatgtt
      541 ctgtcgttat ttcgctccag ttgatgttga aaaattcatc gcctttttta tcgtgttcgg
      601 aatcgaagat gctttggtga aggatgtatt tgccggattc cttgcgcggt aacttgatcg
      661 ctttctggcg ttccaattcc ttgtaaatct gcattgcttc aatcagtacc ggtctgccgt
      721 tcatgaaggg atcacgcaac cttacacgct ggccaactcg accggtaata aagctgtttt
      781 cctcttccac cagcacgata aaaccctttt cctctttttc tcgcaattcg cgcagcagct
      841 ggagttccat atcgcggcgg cgttcagggt agctggtccg ctcagccatg atcagctcat
      901 tgttgatcca tgcagcagtc attgacgccg gtttgccgac gctcattgaa acaacgcata
      961 ttttcttatc catagcgccc ctacaaaaaa gaaaagccac cagcggcggc ttagcaatac
     1021 aactgaaggt agcgcccggt actcagactg tgccgtccat ggaatatttg aaaagggatc
     1081 catccgtacc gggcgtgtga tgattctgac tgaagtcact tgtcagttgt caatcattta
     1141 tcattaaaaa taatatattt attagtgcat gatgtttgcc atctcatagg catcagccag
     1201 taactccatc tctgacttgt tcagcaaggt gaattctttc ttgcctccaa ccacaccatc
     1261 ggcatgaaca gggaccagcc atgggtattt ttctcttact tcagccggag ctgcatgctg
     1321 gtggtgccat ctacaaagtg gcaattgctt tttatgacaa cccggcgcgg tacgaccggc
     1381 gatatggtgc agagacacct cattagatat tactccatgc atatagcagg caatgcaggg
     1441 gagagcgcca agagcattgg cgatgcgccg ttcctccgcc gttggtgttc tccccttcaa
     1501 gccacgagat tttattttta ccgcgctttt ccgcgttttg ctggctggtg ggcgctcttt
     1561 ctgtttagcg atacggcggt cgatagaatc ccgcattttc tgatattgcg attctctcca
     1621 ggcggggtca gccaactttt cccgttgccg agcgatcgct cgttctctgg ctgccttctg
     1681 ccactcgcgg cgctgttcaa gtttttgttc gattgttttc atatggcaaa aaaaaggcgg
     1741 cctaatggcc gccaatgatg tcaaggagtt aagtaatggc aacgtcttcg tagttgacaa
     1801 aaactgcggc tcaattatag caatcaatta gagcaatggt agatattttg ttaatcgaga
     1861 atcacatttt ttcacttcag tacctgtgtg ctatactctt tcttgattga ttggatgcgg
     1921 aatacaaacc cgctcttttg tgcagcctgg ctccttgcca ggctttttta tttcatcatg
     1981 gaagctgtta acgctttgga tcttgctgaa ctgattgaaa gggcattgtt taccttaccc
     2041 aggagttcgc caaattccgc catcactcta gtaagccctc gccgcgcttc ctcctccgtt
     2101 gcattcatca caaaatgttc agcactccgc atgcttttaa cggggaacgc aacagatatc
     2161 gagtcgatat caggcatcct atcgctcaat tttacggtga caatgacaga tggtgactga
     2221 atttgagagc ttacagacag caccacatat tttccgtcta ttttgaaatc cttccgcatg
     2281 tgtcaccata aatatcaaat aattagagca atcaagtaaa aatgaacggc taatcgccat
     2341 cttccagcag gcgcaccatt gcccccgttt cactatccag gttacgaatg tagttcatga
     2401 caatatttac gttggtccag ccaccagctt gcatgatctc cggtattgaa actccggcgc
     2461 gggccatatc tcgcgcggca ccgacacggg cactatgtcc agaccaggcc aggtatcgct
     2521 gaccagagtc atcttttgcc ccgtaaatca atcggtgagt tgcttcaaaa atcccttcca
     2581 gggcgcgagt tgatagctgg ctggtggatg atggcgcggc aacaccattt tttctgacgc
     2641 ggcaaaacag gtagttattc ggatcatcag ctacaccaga gacagaaatc catcgctcaa
     2701 ccagtttagt tacccccagg ctaagtgcct tctctacacc agcggtgcta accagcgttt
     2761 tcgttctgcc aatatggatt aacattctcc caccgtcagt acgtgagata tctttaaccc
     2821 tgatcctaga aatttcggct atacgtaaca gggtgttata agcaatcccc agaaatgcca
     2881 gattacgtat atcctggcag cgatcgctat tttccatgag tgaacgaacc tggtcgaaat
     2941 cagtgcgttc gaacgccagc gcctgttttg cacgctcacc ggcatcaacg ttttcttttc
     3001 ggatccgtcg catgaccagt gaaacagcat tgctgtcact tggtcgtggc agcccggacc
     3061 gacgatgaag catgtttagc tggcccaaat gttgctggat agtttttact gccagaccac
     3121 gcgcctgaag atatagaagg taatcgcgaa catcttcagg ttctgcggga aaccatttcc
     3181 ggttattcaa cttgcaccat gccgcccacg accggcaaac ggacagaagc attttccagg
     3241 tatgatcaga aaacgcctgg cgatccctga acatgtccat caggttcttg cgaacctcat
     3301 cactcgttgc atcgaccggc aatgcaggca aattttggtg aacggttaac gaattagtca
     3361 tttattatgc gtgattttaa cagttaagcc ttgttcatga agtatctcat caataattcg
     3421 aatcacatcc cctggtagat catcgttctc gaaatatctg tgatcgggga tttctacagt
     3481 gattggggtt gctgctttat gcaaaatccg aacaatcttt ttttcttttt tgcatgttgc
     3541 atattcagcc tcttcgatat ctatccagct cgcaccatca aattgctggt aaacccgagt
     3601 gtatagtaag cggtttcgct ttacttcttc tgctaactcc caaattgcaa cagccatagc
     3661 acgttcattt tcattcgttc cagggttagc agctttttgt tttgcgtcgt tcagtaaggc
     3721 gtttaccttg gcatctgtca ggccattaaa cattcatcac ctcatgatca agttaaatta
     3781 ttgttttcag cagagtgtac aggattggct ctgcctttac ctggttatgg ttctcgtcat
     3841 agaaacgcca gcgaccgcgc gtgcgttcta ttttctcttc accgcgagat aatgacagtt
     3901 ggtaactatc acgctcaaac cattttgccc gccagtaacc acggtttttc tcaagctcaa
     3961 gatgagtgga cactttagca gctgaatatc ccatttttca cctctgattg attggtggtg
     4021 ctaagtgcgc tacgcgaaat ctggagcact aacactgcca acatttcgca gattttacgt
     4081 agcgcaacct tgatcaaatg atcaagtgat cactatttga cctgataagg tattgaactg
     4141 tatggattta caggtaaatt aatcatgttc aataaccctt aagataactt cgtataatgt
     4201 atgctatacg aagttatcag gtccgaagag gagtttacgt ccagctgtgc acaaaaatca
     4261 ataattatta gtgcaataaa tttagagaga aaaatcccac tccaccagct aaaaactgga
     4321 ttgtttttca tagttgtttg acaattgctc taataaatta tagttttgcc gtcgttacgt
     4381 aatacgactt tggattcact atttaatgtg tcttcagcgt tgtagagcgg ctcagaagga
     4441 aatgagcaaa cagggaaacc ttatacaacg gcattacagc tatgcattgc tcatcttaca
     4501 cacagcgcaa tgttgttaga ttaccccagc atggatcatg ggtgaaacag taggtcagag
     4561 cttcaggctc tgtgttgtca atacagtgag gcataattat ggctttcatt caaccaacca
     4621 tcgacgacgt tagacattgc tctaacgctt tatctgtaga ccctgccgaa accgacgctg
     4681 cccgcgccat tgctgaacac tactcaaaga tatccaatca ggagtaccgc atcacccaag
     4741 acgacctgga tgacctcact gacacaatcg aatatctcat ggcaactaac cagttggact
     4801 cacaataaat gcactaataa atctattatt tttgtttgat ccctctataa tataggtcag
     4861 taatgaccgg ttttctcagc cgggcgttat tgaccatgtc aattctggag gaggatcaat
     4921 gataaattat gtctacggcg aacaactgta ccaggagttc gtcagcttca gggatctctt
     4981 tctaaaaaaa gctgttgcac gcgcccaaca cgttgataca gccagcgacg gtcgtcctgt
     5041 acgcccggtt gtcgttctac cgttcaaaga aactgacagc attcaggctg aaattgataa
     5101 atggacttta atggcgcggg aactggaaca gtacccagac ctcaatatcc caaagactat
     5161 tttatatcca gtgcctaaca tccttcgcgg tgtgcgtaag gttacaactt atcagacaga
     5221 ggctgtgaac agcgtcaaca tgaccgctgg ccgcattatt catctgattg ataaggacat
     5281 tcgcatccaa aaaagcgcgg ggatcaatga gcacagtgcg aaatacatag agaacctgga
     5341 agcaacaaaa gagctaatga agcagtaccc ggaggatgaa aaattccgta tgcgcgtaca
     5401 cggctttagc gaaacaatgc tgcgcgtcca ctacatttcc agtagcccta actacaatga
     5461 tggtaaatca gttagttacc atgtgccact gtgtggcgtg tttatctgcg atgaaactct
     5521 ccgtgatgga attatcatca acggtgaatt cgaaaaagca aaatttagcc tttatgactc
     5581 catagaaccg atcatctgcg accgctggcc gcaagcaaaa atatatcgcc tggcagatat
     5641 tgaaaatgta aaaaaacaaa ttgccatcac tcgcgaagag aaaaaggtta agtcagccgc
     5701 atcagttacg cgcagccgta aaaccaagaa ggggcagcca gtaaacgaca accccgaaag
     5761 cgcgcaataa attatgcccg gcatcaaccg ggcattcttc cattattcag ccgccaccgg
     5821 ttttaacaag ccagcatcga gcagtttacg cgtcaaccac tgctggcctt tacccgttaa
     5881 ttgaggcgtc aaccgtatct ggtagccatc ttcatcatcc agcaccactt ctttcaccgt
     5941 gaaatacccc gcgttgatgt actgctggaa cggcacattt ttacgtccac cggacgctat
     6001 caggatgccg ttctcccgta accaggcaaa cagcgcgttt tgcttaagtc caacaacctt
     6061 tgcaaaattc ccaatcagga tccctttagc tactgatacc cggtcggcaa aatcgacttt
     6121 aggagcggcg gccaccagct gctgatttag ctggtgggct ttctgttcca gaagctgctt
     6181 ttgttcagcc agttcggcag ccaggcgcag agcttctgga agcgtctggg ggattgcaac
     6241 cggttgctgt tctttttgtc ggaagtagct gtcttccagt ttttcaaaga atgcccatgc
     6301 ctgatcggtt tcgatcattt tagcgtggcg ggctgcgccg cgttccgtcc agaggatgag
     6361 tgagcgggct ttaggagaga taggattttg tgagttactt aaagtcactc gcaaattgtt
     6421 caaatcatct ccaataactt tatagaaatg cttgccttca caaaaacgtt cagcattacg
     6481 ggaaaagttc ttcttgatgt tatccgcatc tgtcccatac cccttagcaa ggatcttagt
     6541 tgtcactaca cgcactccca gccattccag aacgggaatt tcatcagact gattctgaac
     6601 aaccaccagc tccgattcct gaactgaagg tgcatgagtt ttttctgatt taacgttagt
     6661 tgctttcatt ctgtgtgcct ccttgcgtgt ttcggctgcg acggttgcgt aattcagatg
     6721 cccctgttcg agcaggtatt cgcggatatc agacaacaga atgcggtgaa cagcgttctt
     6781 gtcctttctc cggtaaagtt gtttggtgat catgaagtag ttggcaataa cgcccggtat
     6841 atccctggta ctgatacagg atgtgtgctg ttcaattgcc tcaatcattt cttcacgggt
     6901 gactaatgac gttctcatag tccctcctga gcagaagcgt taacagggag gcaccagtaa
     6961 ctgagagagt tgcgcgaatc agtggaaaag cgggcagaga aaatacaggg agcatccgga
     7021 agctgagaac gtgcctcatc ttctgtcggt gcaataacga agtgatagtg acgtttctgg
     7081 caggaataga agcgccagat gaattcagga tgagttgggg tagggacagt agccataatg
     7141 gcagcctcct ttgactaagt taaggagcta ccgcgtgagg tctcaatctc aatggcggta
     7201 gcactgactg ggttgagact accggcgtca aagggaaccg gcctgccttt cggcagccca
     7261 gccagcacta ccattgattt cggagctaaa cgctacgtat ggctgtgcga tggcatgaca
     7321 caaaaaaaga cgctttcggc gtctgtgtcg cctttgacat tatccggggt ctcaatcccg
     7381 gcacccgttt taatgaggtg cctgataagc ataaaccgaa aatgcctcaa ggcgcaagag
     7441 gtcaggttca atgtaacatc ggtagctaaa aaacacaatt tattagagca aatattcatt
     7501 cattaagcca tgccagagct tcatcaacct gcgcttcgtc ttcgacgcta agcacttcat
     7561 cctggggaac ataatcagcc agcatagcga aacaatatgt atcccaatgg tctggtgagt
     7621 gcaggttgag ttttttcttc atatcctcct tactcatcac tttccattga cctgcggagt
     7681 taatccctac agggattttc gacgcttcct caatagtttc attaccctta tccagtctca
     7741 tacgaccaga ttttacggcc tctgcggctt gaacgttggc ataagcacgt ttatcaaagt
     7801 acaggctctt atcttcacgg ctatgcatct ttttacccca gcgtatacgc tgtacggtaa
     7861 taccataata ctcgtacatc agatccgccg ttgctttacc caggccatcg ccgtctatcg
     7921 ctatggtgat atttgggaat cgctcaggat tgcattctgc gaaaattttg gcggcaagct
     7981 gcgtttctgt aacgtctgtg tattccagca ttcgatagtt gattacacgg cgtttatttc
     8041 gctggccgga caccatcatg atattgataa cggacttatc ccgtcccgta ccaccagcaa
     8101 cgtccacaca tgcaagccag ccccatcctt tggcaatctt gactttccgc cgcgttgcac
     8161 gttcaacctc atcacgtcca agaaggaagc catcctgtga tttagggaat aggccgcgta
     8221 ccttaatcat gtacataggg ttatcacgcc cgccgtactc cgccagcttc attttgataa
     8281 atgctggcgt taccagcggt gattcctcac tgttaagcgt gatcgccgta taaacgccat
     8341 cagggttacc aggacgcttg gccagtttat ggtgtgtatc gtagaaatag ccgcttgggc
     8401 gtgtaggctg tgacagcaat aagatgcggt tatcctgtcc ggtaagagca ccggtgatga
     8461 taccgaaagc tctatcactg acaccggagg cttcatcgat aatatacaga agatgatctg
     8521 cgtgttcacc ggcgagagct tcttcacttc ccagacgaaa gcccttcggt actacagtcc
     8581 atacaccttt accagtaacc tcatagaaag cggtttctgt cagaacaaaa taatcagcaa
     8641 gccatggaaa acggctggtg gcagtagccc agtttatctt gatgtacttg aatataccgg
     8701 tcattacctg ctgaattttg ttcgcaacga taatggctcg ggcaccggga tacatgatta
     8761 tgaacaacat gatcatgata gaagtcatgt ctgatttccc ggtaccgtga ccagacgaaa
     8821 cagatgtctt gctaccctgt tcctgcacag actcaataat cagatcctgc tgccaggtag
     8881 gtgttttgcc gaacaaaaca tcagcggccg caatccagtc ataacgatat agcgccacca
     8941 gctcgcgcca acgtggatcc gttacgcaac ttctggccat taatcatcat ccccgtatag
     9001 cttgcgggta acttcttcgt cttcctcctc gtcttcgtcc aggtcttgtt ccagccatgg
     9061 gtcgtttgat acaccttcat tatcaacatc tccataaccg cctgtatcaa cgatatcggc
     9121 gatttcttcc ctacgctgct caatccacaa tgcggcatca gcgcggcggc tggcggcccg
     9181 ttctcgcgcg attttatcca gatcttcaag tgatggagcg ccagatgctg tttggttttc
     9241 ctcatcatcg gtattggtct taggagcacg cagatcggct ttgatttgct ccagcatcag
     9301 gggcggcact ttccctccat gcgcctcgat gaattcagcc gcttccagca ctgaccagtt
     9361 attttcacgc tttcgttcgt atgccagctt aacaatgcca gcttgcccca tagacaaagc
     9421 gtgcttttcc gcctcccggc tttcttttcg atagttattc cggatgctgt aaatggtgtt
     9481 gatcaggctg cttatctgcg cggaacagct gtttagcatg ctcgcgatac ggtattcagg
     9541 cggagtacct tcatcatcgt ctttttgctg atcgcgcatt tcctgcacca gacgaataca
     9601 cgtatcccta gcgttctcca gcataaggag atgagaaaga gacttttcca gaagagtggt
     9661 ttccagaaca tcggccccgg accgacgcaa catagcgcgc gcggccttcc gcgcttcaac
     9721 gttatctatc aggtaatcgc cagcttcgaa ttcaaagcgt tcaccatcat catccagggt
     9781 gtcgcgttcc aggcgatcac gtaaggtccg gtgggcgcgg gtgatcacgt catgatcatc
     9841 agaacgatca tttatgcgct tattttggcg cttcgcattc tcgactgcgg cactgacaac
     9901 ggcattaacc ctttgttttt ccgctatttc agccgcaatg tgatcacctg catgttgatc
     9961 attagagtga tcaatgatca tgctttttag tggcttcctg actggcttat ttggcttgcg
    10021 gctgtccgct gtcctggtgt cttctttgaa ggcacggaga taacgacgtg cagtattagg
    10081 gttaagatta aactcggcgg catactgtgc gatggtgtaa ccaccatctc gcgccaggcg
    10141 agcaaaattc ttcttgtgat cgtcccaggt cacttatgct tcctttcgta aaaactcttt
    10201 ttgacgcgag ggtaacgaaa gtcacatgtc aaaaggcccg gaacgggcaa gcaatcaatc
    10261 agatacgtgc ggatgtggca ttaccgtaat gacggtgctg acgggccacc ttattgaaaa
    10321 gttgacgcgc cattacccaa ggctggtgct cccggcgttc cttttcgtcc tgcgtcatat
    10381 agagttcgtt ctggagtttt tcatcaaacc ggcgcggagc gcggctacgg cgaaagaatt
    10441 caggattcag agagtggatc tgaaatctac gtgggcgtgt actgtcatca atcaaaacag
    10501 acgaatactt agacacagcg atagccttta agcgcagata aacatcgcgc ttatcgacat
    10561 ccagatgcgg gtattccttt tcaagaattg ctgcgagttc tttcgctgat agaagagatt
    10621 tagtgcggat catgtaatcc gcaatctcgt acgatgttat tcgtgagtga tttatttcca
    10681 tgaagtggcg tccctgccag ttaagtaaca tcctgtcacc tactgattag cccatgtcaa
    10741 ctaatcaacg tggaatataa taccctcgat taaagaaata gcaatacatt agagcaattt
    10801 tatctaacgc tcgacgagtg acttgtgata gcgccgactc caagcgcgta atcaaagaac
    10861 aatcgttgat gcatcgccag cctgccgtgc gtcttctccc aattatcgcg gtcacgctca
    10921 atatcacgct ggcatgactg gcacagagga attgcgtaaa tgtcatgcgc gcataatcga
    10981 ctatgacgaa cgatataagg cgtaatgcga gcgccagctc ccgcagctcc acatccacag
    11041 cacggacggg aagcaacaaa gtccatgtac tcgggtaatt ttagcgattg cagttttggt
    11101 attttgaaat gcgccatgcc agggtcggag tcaacatcca cagggcatac ttttgcacgc
    11161 atcggcgcgg cgcgttcttc catcatctga acatatgctg tagcacgatc gtcgtacggg
    11221 cgaatatccg cctctttcaa aggtccgcta tcctgcggag cagccttcat cttatttatt
    11281 gatatgcggc aaacttcttc cggcatcagg tgcatcatgt tgcgcatgaa agcccaccag
    11341 cacagttcct gaatacttaa atcatggcca tctgaaagcc ccatttcctg acgggcgaca
    11401 tccagtatcc agttaacgcg attattatgc agcgtttctt tcagctcatt aaaaccacgc
    11461 atccggtaat ggttatcgtg atgccagcac aacaacaccg cgctattgtc tcgttctgcg
    11521 tggacaatat ggttgtcaca ccagctacga tctgcggcct ggcattgccc ctctttcctg
    11581 cgcaaccacg ccaccagcgc gtcaattcca ccaatacggc gaaacagttc atcgctgtta
    11641 aaaaacggct gcaacgcctc atttgttgcc atagtttgct cggaaacaac gaggccgtcg
    11701 tccatgtgct cgattaactc acgcggcacc ggctccataa taaatttacg gccagcctcc
    11761 accagctttc tgacttcctg atccactttg aatgtggcga cgccaagctc tttttgtaca
    11821 aagggagtaa ttacggcttt cacatcacac ctttcatcac tgattgggct ttatctgctg
    11881 cccggcattc tctgtttaag cacaaccatt tcctgacggc ataacacagc aatagcagtc
    11941 ctagctctaa tttgcttacc aaccaggtat tgctttacct cgcggcgact cacgccatca
    12001 agaagcatct ttaacgcttc acgggacaat ttgttgtatt tgcgtgccat taatctactc
    12061 cgcagaacca tacaatctac gtaacgtgtc ggcgacagaa gatacagata tctcgccagt
    12121 cgcagcgcct acagtaaggt ctgccagttc aggtgaatca aatacctgca ccccgttacg
    12181 gcgtagaaat agcagcgcgc tgtttagcgc ggtacgctta ttggcatcat tgaatatatg
    12241 ccctctcgct gtagccacca ggtaggtggc ggagacttcg aaaaggtcgg tgatctcttc
    12301 gtaggcaact ctggcctgaa ctctcccgat aatggcctct gccctgcccg gatctgacat
    12361 gccaggcagg ccgccgtagc ggcttatatt cgcatcatga agcgcaataa gttcttccgg
    12421 tgatatatgc ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac
    12481 agggatgcaa attcagcatc cagcgccgct tttttgtagg cttcgaaagt agccttgctg
    12541 acaattactg ctggctcacg gcctctgcgg gtgatttcaa cctcttcccc ggcttcaaca
    12601 ttgttgagca cttcagaaag gttgccgcgc gcggtacgga agttaatgga ttgcataaac
    12661 acctcgtgta ctcgttatgt gtacacaatt ataaacttca caggcataaa gcaccagcac
    12721 tttgcagctt aaatcaccgg acaatcatca aattccccac ttcgggcatc attgatgaca
    12781 tgagtgatca caccaaaaac agcattacta cccgtgtaac catcgtcatc tactggtaac
    12841 gcctctttct tcccggtgct taaatcctcc aggtgctggc gcggatactt tcggtatctc
    12901 tttatgcgat attcaccctc catagcgcag acaagcagcg aaccatcaac cggagtaagc
    12961 gaggaatcaa ccaccagcaa agcaccctgc aatattccct cacggtgatg gctgtctgcc
    13021 gcccgcatga agtaggtcgc tgatggatgc ctgattagtt gctgatcaag agaaattctg
    13081 ctttcaacat aatccgccgc aggagaaggg aagcccatag cgttttcacc tcaataatac
    13141 tgttcattta tacagcatac attaaagaga cacctttggt gcaaactcgt tatgtacacc
    13201 aaccaccgca gatgattttg tgcgctttgc tactattcat caccaatgga tcagcgtaat
    13261 ctcattgcca atctgcagaa ttttatccac cagcgatgtg attttttctg ccaggtcatc
    13321 attacttacc caatcctggc gggcagccag aacgggcgac aaggctaata ttaactctcg
    13381 tcgtaatgat acgttccttg ctacaggcgg aatacgtggt gctggtggtg gtttaagccc
    13441 ctcgctcccc tgtatttctg gagtattcgg ctgatagcca ttagttattt tcggcggtat
    13501 tggacgagga cgaaacatcg cgctacctcc tacactcagg gcaggtgttc ccttccgcaa
    13561 agtaggggaa actgctatca agttcatcac accatctgcg atgctcgtag caccaaaaag
    13621 cctcccacgg taaaccaaaa gacttcatcc accgggacac tcgatctggt atatctgctg
    13681 gtggcagttc ttctggcaag aatttgcctg ataattccct ttccgcccgt gccagcattc
    13741 cttttagact cgcattctct ttttcaagaa tctctatgcg cgcctgtaac tcagctttcg
    13801 ttggcatggt ccgcctcatg cttttcagcc accagcggca ataaagccct ggccatctta
    13861 tgaaccaaca gagcatcgat aatgccaagc gtatgccccg gcttaatgtt taatgccgcc
    13921 tcaaggtgac acctttccag gtcacttttc tcggcttgtt tatgatgatc tggcgtaata
    13981 acgtcgccca aaacacggct aattctttct cgtaattgcc gggtgcctgc acacttgatc
    14041 gctgtatcgt ggagacggtt aaccagttcg cgataaacat gcggcttaat gcggatacgt
    14101 tcaccggtga cgccctttcc tggcgctggc accgaactat ccggaatatc cggatagttg
    14161 ccagcctcgt aagctacccg cagccagtgc atgaatgttt cagtagacac acaaccacag
    14221 tccacatcga tttttccgcg ttgctgttcc agccactgct caaaattcaa tctacacgta
    14281 ttactttcat gttgctcttt ttgtcttaag gccagcacct gttgggccag ttccagaacg
    14341 ataccgggtg acgctaacct ctcaaattcc aataaatagt ttgcgtcagg atgacaagta
    14401 gcctcgcctg caaaaaatac caattgctgt aagtatgctg tcgttagagt agtcatttct
    14461 ttttgcgcca tttcttttca cattccttag tccatttttc aatgttcatt ttggcaatat
    14521 cagtcattcc atcacctaag aaatactttc tccggtacgt cttgcactta aaccacacta
    14581 caacagccac cagccagaaa ataaaaggcc atacagcaat accaactcca gccgcgataa
    14641 agcacaatag ccataaatga agctctccaa cttctgtttg cggcaatatt cttaaagaat
    14701 taagcagcag actgaaggaa tggtcgtatg cattggcggt ataagacatg caatccatat
    14761 aattaaagtc atagcctgcg gctgccgccc ataatgggcg gtcaagaaaa tgttttagtg
    14821 tcatcatata aatttaaggt tcagaccagt tatcttcaat agcaatgctt aatctttgta
    14881 gccattctgc taatttcagc attgcttctc tttcgcttaa accacgagga aaatcatcaa
    14941 gcgaaattgt tggcttgaag cccccgtaat tatccatttc aacagtcaga ttttgctcca
    15001 gcacggtatt ccttacgcgg ctgttgtgtc gaagcaaata tactgaacgt gatttattgg
    15061 ttttatggtc aaactgatat tcggtaagta tcatctggct tttgccatga ctattacctc
    15121 tccacatact tacctcactt aataaaacaa ctccatacgt agttgatgat tttttcccac
    15181 gtaatataaa tatgcactcc agcagaaaaa ccaaaaccta caattactga aaaaatcaaa
    15241 acatttactt ttgacattat aaattttctc tcggtgtcgt aggtgatagc accataattg
    15301 ataatttagt gagttagcag ttccattttt tggatgattt ccgcatgagc atcatcgtta
    15361 tcaacactta actcgtttaa tgcctctcgc accacatcaa cttcttctgg ttggaagaaa
    15421 tcatctcggt agtcaccaaa tagaaccgaa acaagcctac caccagcaac atcaagattg
    15481 gcgctaacag gtggctcttt accatcctca aattcgacta caaaagttat ttttcccatc
    15541 gttaccacca gcgacaaatt gaatacaaac ccagtgctgc cgccatcaca attcctaccg
    15601 tggtgaatgc ttcaggccag ctcattatct cacctccagt ctccataccg cctgaccaat
    15661 ccggctggca tgggtatctt tggatgctgt tccgtcttta gccatctcca taagaatttt
    15721 gcgcaaatct gccgaacgcc attcttcatc aggaaattcc ttctccattg ccaaccgcag
    15781 attccaggtt gccatcctga atggatattc cccgccgaga gctttatctt gcagggcagc
    15841 ccgggaacgc atcacctgca aaaccttctc ttttacatcc atcatttcgc ccccttcgga
    15901 ggttctggca acggcatcca gaacaagacg ttccctaacc acgataaagt gccgtcgctc
    15961 aactccacgt attccccttg cacctgtcct gccatatact cgccgtgctt tgaataaatt
    16021 aaaatccaat catcttgagg gggcattcgc tcactacagc ttatccaacc atcc
//
LOCUS       DAZUFB010000112        15739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZUFB010000112 DAZUFB010000000
VERSION     DAZUFB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.112"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2429
                     /locus_tag="ACHY3M_003988"
     CDS             <1..2429
                     /locus_tag="ACHY3M_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4318811.1"
                     /translation="GCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNAL
                     INNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYPIEKEK
                     QITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQG
                     DNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAK
                     LTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNR
                     IIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYT
                     TAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNS
                     LGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNE
                     YINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRD
                     FDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTA
                     SWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLN
                     ASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVS
                     SYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSH
                     PPLGISVKDKTSHKELGLVADGCFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTG
                     TVILPCISQN"
     gene            2445..3193
                     /gene="yqiH"
                     /locus_tag="ACHY3M_003989"
                     /pseudo
     CDS             2445..3193
                     /gene="yqiH"
                     /locus_tag="ACHY3M_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            3195..4259
                     /gene="yqiI"
                     /locus_tag="ACHY3M_003990"
     CDS             3195..4259
                     /gene="yqiI"
                     /locus_tag="ACHY3M_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318812.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNQYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4302..4502)
                     /gene="glgS"
                     /locus_tag="ACHY3M_003991"
     CDS             complement(4302..4502)
                     /gene="glgS"
                     /locus_tag="ACHY3M_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFS4318813.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            4771..5400
                     /gene="yqiJ"
                     /locus_tag="ACHY3M_003992"
     CDS             4771..5400
                     /gene="yqiJ"
                     /locus_tag="ACHY3M_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFS4318814.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            5427..7088
                     /gene="yqiK"
                     /locus_tag="ACHY3M_003993"
     CDS             5427..7088
                     /gene="yqiK"
                     /locus_tag="ACHY3M_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4318815.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            7327..7386
                     /locus_tag="ACHY3M_003994"
     CDS             7327..7386
                     /locus_tag="ACHY3M_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4318816.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            7703..7762
                     /gene="ibsE"
                     /locus_tag="ACHY3M_003995"
     CDS             7703..7762
                     /gene="ibsE"
                     /locus_tag="ACHY3M_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFS4318817.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(7882..9315)
                     /gene="hldE"
                     /locus_tag="ACHY3M_003996"
     CDS             complement(7882..9315)
                     /gene="hldE"
                     /locus_tag="ACHY3M_003996"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFS4318818.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKDALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(9363..12203)
                     /gene="glnE"
                     /locus_tag="ACHY3M_003997"
     CDS             complement(9363..12203)
                     /gene="glnE"
                     /locus_tag="ACHY3M_003997"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFS4318819.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(12226..13527)
                     /gene="ygiF"
                     /locus_tag="ACHY3M_003998"
     CDS             complement(12226..13527)
                     /gene="ygiF"
                     /locus_tag="ACHY3M_003998"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFS4318820.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            13769..14389
                     /gene="ygiM"
                     /locus_tag="ACHY3M_003999"
     CDS             13769..14389
                     /gene="ygiM"
                     /locus_tag="ACHY3M_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFS4318821.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            14453..15691
                     /gene="cca"
                     /locus_tag="ACHY3M_004000"
     CDS             14453..15691
                     /gene="cca"
                     /locus_tag="ACHY3M_004000"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFS4318822.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         4102 a         4011 c         3746 g         3880 t
ORIGIN      
        1 ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc
       61 gtgaaaacgt taacctttcc caatttgaaa ctgatggcca attacccgta ggtaaatact
      121 cattaaacgc tcttattaat aataaaagga cgccaatcca tctggacctg caatgggtat
      181 taattgataa ccaaactgca gtttgtctga ctccagagca attaacatta ttaggcttta
      241 ctgatgagat tattgaagag gctcagcaaa acctgatcga tggttgttac cctatcgaaa
      301 aagaaaaaca aattacaact tatctcgata aagggaaaat gcaattatcc atatctgcac
      361 ctcaggcatg gttaaaatac aaagatgcaa actggacgcc tcctgaactt tgggatcatg
      421 gtattgctgg ggcatttctt gactacaatt tatatgcctc tcattatgca ccacatcagg
      481 gcgataattc acaaaatata agttcctatg ggcaggctgg ggttaatctt ggggcctggc
      541 gcctgcgtac tgattaccag tacgatcagt catttaacaa tggcaaaagc caggcgaaca
      601 acctggattt tccgcgtatt tatttgtttc gcccaatccc agcaattaat gcaaaactaa
      661 ctataggtca atacgatact gaatcctcta ttttcgactc tttccatttt tctggcgttt
      721 cgttgaaaag cgatgagaat atgttgccgc ctgatctacg tggttacgca ccgcaaatca
      781 cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt attatttatc
      841 aagaaaatgt tcctcctggc ccatttgcta ttactaattt attcaataca ttacaggggc
      901 aactggacgt caaggttgaa gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta
      961 atagtattcc ttatctgacg cgtaaagggc agattcgcta caccactgct atgggtaaac
     1021 cgaccagcgt tggtggtgat tccttacaac aacccttctt ctggactggt gaattctcat
     1081 ggggttggct gaacaatgta tccctgtatg gtggttcagt tttaacaaac cgtgattatc
     1141 aatctctggc tgccggcgtt ggctttaacc ttaactcatt gggttcatta tcttttgatg
     1201 tcacacgatc tgatgctcag ttgcataatc aggataaaga aacgggttat agctaccgcg
     1261 ctaactattc aaaacgtttt gaatctaccg gtagccagct cactttcgct ggttaccgtt
     1321 tctctgataa aaactttgtg acaatgaatg aatatatcaa tgacactaac cattacacga
     1381 attatcagaa tgaaaaagag agttatattg tcacgtttaa ccagtatctt gaatcattaa
     1441 ggttaaatac atacgtaagt ttggctcgta atacttactg ggacgccagc agtaatgtga
     1501 attattcatt atcacttagc cgcgattttg atatcgggcc attaaaaaac gtctccactt
     1561 ctctaacatt tagccgaata aactgggaag aagacaacca ggatcaactg tacctaaata
     1621 tttcgattcc ctggggaacc agtagaacat tgagctatgg tatgcaacga aatcaggata
     1681 acaagatttc gcatactgct tcgtggtatg actcttccga tcgaaataat tcctggagcg
     1741 tttctgcttc aggcgacaat gatgaattca aagatatgaa agcgtcacta cgcgccagtt
     1801 atcagcataa taccgagaac ggtcgtctct atctctccgg gacatcacag cgagacagtt
     1861 attattctct gaatgccagt tggaatggtt cattcacggc gactcgtcac ggagccgctt
     1921 tccatgacta tagtggtagt gctgactcgc gttttatgat cgacgcagat ggtgcagaag
     1981 atattccgtt gaacaataaa cgcgcggtaa ctaatcggta tggcatcgga gttattccat
     2041 cagtcagcag ttacataaca acatcattaa gcgttgatac ccgaaatctt ccagaaaatg
     2101 tggatatcga aaactcggtt atcaccacca ccttaaccga aggtgctatt ggctacgcca
     2161 aacttgatac ccgcaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
     2221 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
     2281 ctgatggctg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
     2341 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
     2401 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     2461 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     2521 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     2581 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     2641 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     2701 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     2761 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaatgt
     2821 gatgcaagtc actatgcagc acgcattgaa actattctgg cgaccaaaag ctattgaatt
     2881 agaagatgat ggggtaatgt cttacgaaaa agttgaaatc atccgtagaa atgatggttc
     2941 gatccgcttt aacaataaga tgccttatca cgttacgctg ggttatatcg gaacaaacgg
     3001 aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc ccttatagcc atgcagatac
     3061 gcagtttaaa aatgttccgt cagcttttca ggttggttat atcaatgatt ttggtggatt
     3121 aagtttttat gaaataaact gcccgacggt taataactct tgtaatgttt ccgtagccaa
     3181 acgagataaa taatatgcgc tacttattaa ttgtaattat atttattata ggttttagtg
     3241 cattacctgt atgggcaatg gactgctatg ctgaacatga agggggaaat accgtagtca
     3301 ttggttacgt accaagaatt tctatcccca gtaatggtaa aaaaggtgat aagatctggc
     3361 aaagcagtga gtattttatg aatgtcttct gtaataatgc attacctgcc ccatcacctg
     3421 gagaagaata cccatcagcc tggactaata taatgatgtt tttaccaggc ggtcaggact
     3481 tttataatca gaactcctat atttttggcg taacatacaa tggagttgat tatgattcga
     3541 ctgcccctaa tgctatcgca gcaccagaat gcattgatat aaaaggagcg ggtaccttta
     3601 ataatcaata taaaaacccc gctgtctgta gtggaggtcc tgagcctcaa ttatcagtaa
     3661 cctttccagc tcgcgtacaa ctctatatta aattagcaaa aaatgccaat agagttaata
     3721 aaaacctggt gttacctgat gaatatatcg ctctagagtt taaaggtatg agtggcgcag
     3781 gagctataga agtagacaaa aatttgacct tcaggattcg tggattaaat aatattcatg
     3841 tccttgactg ctttgttaat gtcgcactgg aaccagctga tggcgttgtt gactttggta
     3901 aaataaattc ccgaacaatt aaaaatacca gcgttagtga gacgtttagt gtagtcatga
     3961 caaaagatcc tggtgcggcc tgtactgagc aattcaatat tttagggagc tttttcacta
     4021 cggatatttt gagtgattat agccatctgg atatgggtaa tggactgcta ttgaagatat
     4081 ttcataaaga tggaacagca acggaattta accgcttctc acaatttgct tctttttcat
     4141 cgtctagtgc accttcggtc accgcaccat tcagggcaga acttagtgcg aatccggcag
     4201 agactgttgt tgagggaccg tttagtaaag acgtcatcct caaaatcacc tataactagc
     4261 atctaataca aacactaaaa cgggccatca ggcccgttat atcagtgctc taactccagc
     4321 tctcttgcct gcatcatctg ttgacaatac caggcataac gtgcgcaaaa ccaggttcta
     4381 tgttcttcat ccatgttacc agtaacggca agaatatcga ccgggcgacc ttctgcgtgc
     4441 attctggcaa aactacgcgc caggaaatcg aaattattta aagaattaag actatgatcc
     4501 ataagcacat cctccggtta tcgcatacct tttaaggcca tatgcttttt ctgatttcag
     4561 tattccccaa tcagtgtagt gcctttaaac ataacgtgcg taaatatatt gtccccgatc
     4621 acacttttta gtgaaaaata aaaaaccata ttaaaaaact ttctcttcca gaacttctct
     4681 aacatgtgtt attattaaca aagataaatg taagcaagga aactatccca aagtaaatat
     4741 tgcgtcacta aatggacatt ggagtgacat atgattttat tcgccgacta taatacccct
     4801 tatcttttcg ctatttcctt tgtactgttg atcggccttt tagaaatttt agcgcttatc
     4861 tgcggtcaca tgctctccgg tgctcttgat gcacatcttg accattatga ttccataacc
     4921 acaggtcata ttagccaggc acttcattat ctcaatatcg gaagattgcc agctctcgtc
     4981 gttctctgct tgctggctgg tttttttggt cttattggca tcctgttgca gcatgcctgc
     5041 atcatggtct ggcaatcacc actgtcgaac ctgttcgtgg ttcctgtcag tttactgttt
     5101 acgattattg cggtgcatta taccggtaaa atagttgcgc cctggattcc acgcgaccat
     5161 agttcagcta ttacagaaga agaatatatt ggtagcatgg cattaattac cggtcatcaa
     5221 gccacttcag gtaatccctg cgaaggaaaa ctcaccgatc aatttggcca aatacattat
     5281 ttactactgg agcctgaaga aggaaaattt ttcactaaag gagacaaagt gttaattatt
     5341 tgccgacttt ctgcaacgcg atatttggcg gaaaacaatc cctggccaca aatattgtaa
     5401 tttattaatc aaaaggaaat ttaataatgg atgatattgt taattctgtg ccctcctgga
     5461 tgtttaccgc gattattgcc gtatgcattc tgtttattat tggaattatt ttcgccaggc
     5521 tctatcgtcg cgcttcggca gagcaagctt ttgttcgtac tggtttaggt gggcaaaaag
     5581 tggtgatgag cggtggcgca atcgtgatgc cgatctttca tgaaataatc cccatcaata
     5641 tgaatactct gaagctggaa gtcagccgct caaccattga tagcctgatt acgaaagatc
     5701 gtatgcgtgt cgatgtagta gtcgctttct ttgtacgggt aaaaccgtct gtagaaggca
     5761 tcgccactgc ggcgcagacg ttggggcaac gcacgctatc accagaagac ttacgtatgt
     5821 tggttgaaga taaatttgtc gatgccctcc gtgcaacagc tgcacaaatg accatgcatg
     5881 agttacagga tacccgcgag aacttcgtgc agggagtaca aaatactgtc gcagaagacc
     5941 tgtcgaaaaa cggtcttgag ctggaaagcg tttcacttac caactttaac cagacctcga
     6001 aagaacattt caatccgaac aatgcctttg acgccgaagg tttaaccaaa ctgactcagg
     6061 agacggagcg ccgtcgtcgc gaacgtaacg aagttgaaca ggatgtagaa gttgcggtgc
     6121 gtgagaaaaa ccgtgatgca ctttcgcgca agttggagat tgaacaacaa gaagcgttta
     6181 tgacgcttga gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg
     6241 cggcttttga agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac
     6301 aaattcagga aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg
     6361 aacgtgaagt tcgtattaaa gagatcgaac aacagcaggt caccgaaatc gccaaccaga
     6421 cgaaatcgat cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta
     6481 accttgcact cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact cgccagaccg
     6541 cagaagccga tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca
     6601 aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg
     6661 cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac
     6721 aacgtgcgct gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca
     6781 aactggcctt gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat
     6841 ctattgacgg tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg
     6901 atgcaaacac gggtaatgtg gggggcggaa acctggcgga acaagcatta tcagccgctc
     6961 tctcttaccg cacacaggca ccgctgattg actccttgct caatgaaatt ggcgtttcag
     7021 gcggctcact ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg
     7081 tagaataaat tttcagctat gaacgtccct gtcacaaaaa gccggggatt ttttatatct
     7141 gcgttccgct aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg
     7201 gaaggcaaaa ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg
     7261 gagtcagcaa agggctgaaa cgggaaagcc cctccctagg aaggggcctt acagaaggaa
     7321 agggttatga tgaagctcgt catcatactg attgtgttgt taatcgtaag tttcgcagct
     7381 tattaacagc caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg
     7441 ttgaaaaaaa atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg
     7501 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt
     7561 tgcgtgaccg ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg
     7621 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg
     7681 ggccttatag aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact
     7741 gttaagtttc ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt
     7801 gttcctttac tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga
     7861 gatctgtgaa ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
     7921 agtggtcgag caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac
     7981 ttctttactc ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg
     8041 caggatcccg gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
     8101 cgcttccagt gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc
     8161 ccctttcagc cgtttggtgg aggcatcgct gttcacagcg acaatcagac ggtcacccag
     8221 tttgcgagca tttgccagat aggagacgtg cccggcgtgc aggatgtcaa agacaccgtt
     8281 ggtcattacc accttttcgc cgcgtttacg cgctgccgct acagcaagct tcagttcctc
     8341 ttcggtcatc acaccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat
     8401 cggcgaaacc gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa
     8461 gaagcaggct tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac
     8521 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg
     8581 cgctttaccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
     8641 gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca
     8701 tttaccgaca acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta
     8761 gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag
     8821 ttggatcatc tgctgtacgc ttgccagcgc atctttggcg tagtcagaaa gcaccagcgc
     8881 gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc
     8941 gaaaccttct tcaaaatcca ggcggatcag ctgttggttg cgggaaagta cccgtaattt
     9001 ggtgatagtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
     9061 agatttactc agcgcacgcg ccgcatcgtc aatgcccgtc agcccgacca ggcgagcatt
     9121 ggcaccgaga gaagcgatgt tcattgccac gttagccgcg ccgcccggac gttcttcgat
     9181 ggtattgact ttaaccacgg gcaccggcgc ttccggcgag atacggctgg tgggaccgta
     9241 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
     9301 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
     9361 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagttcacg ctctgcggtg
     9421 aagcaatcct ccggcacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
     9481 cgcagcgtag tatacgcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
     9541 tgcgccagta gttccagaat gcgcacgttg tctgaccagc gcgttaactt cggtttttca
     9601 tgagcgtagc gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca
     9661 tcagctttga tatcaaagcg atcgcgatgt ttattgccga gatgagcgcg cattttctcg
     9721 cgcatttccc gcacttccgt ttgcagagtt ttaccttcac gcggcagcgt cataatctcg
     9781 cgacgcaccg cgtcaaagtg cgcggtgagc tgcggatcgc cgtacactac ccgcgcacgc
     9841 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgcttct
     9901 gcggatgtca ccagcattcc cgcggccccg gacgggcgta gccgtgcatc cacttcatac
     9961 aaaatgccgg aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
    10021 taaaactgtc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca
    10081 tggaggaaga tcaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
    10141 tagccgacca ccgcaaaacc acggccttcg cgatcgttga ggtgatttgg cttaccgtag
    10201 cgggccacca tttgaaccca cgcctgctga acgacggcat ctatcatagc ctctgccagc
    10261 caggttaagt gatcgctcac tttcatcacc ggtagcgtac cagcgatatc cgctgcggcg
    10321 atgcgtaaca gctgtgcctg tttgaactga cgcagcgcct caagctgttg ctcttcgtca
    10381 tcttccggca cgcgcagcaa atactggcgc aactcatcgc ggtaggcatc ggtcgccgtc
    10441 ggctggtaaa gggtgtttgg atcgagcaat tcatccagca ataatggata acgcgccagt
    10501 tggctggcaa tcatcggcga cgcggcacac agagaaatca aatgtttaag cgccgcgggg
    10561 aattcactga gcaattctaa ataggtggtg cgggtaacaa tccccaccag caaggcggta
    10621 atgcgcgaca gcgtaacggc agcgtcttca cgcgcacaga catcacttag cagatgcggc
    10681 atcagatggt cgagcacctg acgtcctcgc ggcccgatgg tgcgcttatc cagctctttg
    10741 cggaaatcgg caatcagcgt tagcacctgt ttgcgatcat cctcgctaag atgcgccagc
    10801 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
    10861 agcgactctt cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc
    10921 acattggtca tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc
    10981 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtctgggt ttgttcgtcg
    11041 ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca gatacgccac tcgcaattgt
    11101 tcagcatcgt tttcagaaag cagatgcagc gcggcaatga cgctgagcgt tggcagtaaa
    11161 gagcgcgact gcagcgacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata
    11221 aattcaattt cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga
    11281 cgcacttcac gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg
    11341 atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
    11401 tcgctatcgc ccataatccg cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc
    11461 tcctggtaat aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac
    11521 ggacgcagcc gcatatccac gcgatagacg aagccatcct gcgttggttg atccaacact
    11581 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
    11641 ccctgcgtac aaccatgttc cggccaggca aaaatcagat cgatatctga ggagaaattc
    11701 agttccccac cgcccagctt acccatgcct aaaatcagca gcggttgcgc ttcgccctgc
    11761 gcattgcacg gcgttcccca ctcgcggcag caagcgtcat acagccagtc acgcgcggcg
    11821 acaatcagcg tctccgccag atggctgagc tgctgcaata tgctctcttc agtaaccaaa
    11881 gccagcgttt gcgcccaggc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg
    11941 cgcattaacc cggcttcgtc actcacatta ctgagcgcct cctgcaacca tgccgcgtaa
    12001 tgctgccatt cgtcggcctg cggcggttgg ctttccagtt ccgtcagcca ctctggatgc
    12061 acaatcacgc tgtcctgcac aaaatcacta aaagtaagta ctgacttcgc ctgtgcgcta
    12121 agtgattcct cggctaaagg ctctggcagc cgctcaacaa cggtctgcca gtactgctgt
    12181 aacggtgaag agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct
    12241 gtgcaaccag aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg
    12301 ttgcccggtc gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac
    12361 gacaggatca tagtaacctg ccagcagcag tattgagtca atatcacgcg tcaggcgtgg
    12421 caactgatca tggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc
    12481 atggcgggaa agatggatat cggcaaagcg tttgaaggaa tcgctgattt taccctgcgc
    12541 tttggcatct aaaaacggct gccaggcttt gcttaccaac cattcggtca acgccagctt
    12601 tgccattgcc gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt
    12661 cgcttcgcat tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc
    12721 accgaacagc atcagtgcat ggcgaacaag gccaatggct gccagcacct gctctttcgc
    12781 cgcatcgttg ccgcgtaccc acagttcttc atgatactgc cattgcgcta acgccagctc
    12841 gaacgccgct tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt
    12901 cggtttgatt tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct
    12961 caggctgccc tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc
    13021 gcgcgtgtcg ccgctaagca gttccagttc cagctcacag ataggttcag caaattcccc
    13081 cgctttcacc tccccctgat cgagagcgat ttcaattcgg ctgtcatcga ccgccaccag
    13141 ccatttttcg cgataaaaat cggtgctgaa cagcggttgt acgcgggaag cgagatcggc
    13201 gggcaattcg ccgttcggcc agacttccgt cggtaactgc gccaggtcga gcgtcggttc
    13261 gctcaacgcc acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac
    13321 tttcatggtc atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgccc
    13381 acgcagccag ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg
    13441 ctcgccgccc agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat
    13501 aaactttaat tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga
    13561 cgatgaacgg cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt
    13621 gccatgcaac gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca
    13681 gaggattccg attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc
    13741 cactttccat gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt
    13801 actcgcactt agcgccactg ccgtctcaca cgctgaagaa acgcgctatg tttctgatga
    13861 actgaatacc tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa
    13921 cgccggcgag gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa
    13981 agacagctct ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct
    14041 gcgctcccgt gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa
    14101 tatcgataac acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga
    14161 cagcgtgatc aacggattaa aagaagaaaa tcagaaattg aaaaacgagc tgattgtcgc
    14221 gcagaaaaag gtcgatgccg ccagcgtaca gttggatgac aaacagcgca ccatcatcat
    14281 gcaatggttt atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact
    14341 gccacacctg atcccaacgc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt
    14401 cgttgcactg tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat
    14461 ttatctggtc ggtggtgctg ttcgggatgc attgttaggg ctaccggtca aagacagaga
    14521 ttgggtggtg gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg
    14581 ccgcgatttt cctgtgtttc tgcatccgct aacgcatgaa gagtatgcgc tggcacgtac
    14641 cgaacggaaa tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac
    14701 gctggaagat gatcttaagc gtcgcgatct gaccattaat gcgctggctc aggacgataa
    14761 cggcgagatt atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca
    14821 tgtttccccc gcttttggcg aagatccgtt acgcgttctg cgcgtggcgc gttttgctgc
    14881 gcgttatgcc cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat
    14941 gacccatgcg ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag
    15001 cgccctcacc acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggtgcact
    15061 tcgcgtttta ttcccggaaa ttgacgccct gtttggcgtt ccggcccctg ccaggtggca
    15121 tccggaaatc gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag
    15181 tccgcaggtt gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc
    15241 gccagaactt tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca
    15301 actatgccag cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga
    15361 gtttcacgat ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt
    15421 tgattccatc gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga
    15481 ggctgacgta cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt
    15541 gcgcgaagcc tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt
    15601 taaaggtgta gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagttg
    15661 gaaggaacaa cgttgcccaa agcctgactg agtataaaac taaacgttaa cacgaaccat
    15721 cccctcgccc cttcgggga
//
LOCUS       DAZUFB010000113        15710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZUFB010000113 DAZUFB010000000
VERSION     DAZUFB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.113"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..356
                     /gene="yjbE"
                     /locus_tag="ACHY3M_004001"
                     /pseudo
     CDS             115..356
                     /gene="yjbE"
                     /locus_tag="ACHY3M_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
     gene            470..1108
                     /locus_tag="ACHY3M_004002"
     CDS             470..1108
                     /locus_tag="ACHY3M_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFS4318823.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWGGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1105..1842
                     /locus_tag="ACHY3M_004003"
     CDS             1105..1842
                     /locus_tag="ACHY3M_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS4318824.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1842..3938
                     /locus_tag="ACHY3M_004004"
     CDS             1842..3938
                     /locus_tag="ACHY3M_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS4318825.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3985..4263)
                     /locus_tag="ACHY3M_004005"
     CDS             complement(3985..4263)
                     /locus_tag="ACHY3M_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318826.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4264..4407
                     /locus_tag="ACHY3M_004006"
     CDS             4264..4407
                     /locus_tag="ACHY3M_004006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318827.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4533..4943
                     /gene="psiE"
                     /locus_tag="ACHY3M_004007"
     CDS             4533..4943
                     /gene="psiE"
                     /locus_tag="ACHY3M_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFS4318828.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4987..6462)
                     /gene="xylE"
                     /locus_tag="ACHY3M_004008"
     CDS             complement(4987..6462)
                     /gene="xylE"
                     /locus_tag="ACHY3M_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFS4318829.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6834..7724)
                     /gene="malG"
                     /locus_tag="ACHY3M_004009"
     CDS             complement(6834..7724)
                     /gene="malG"
                     /locus_tag="ACHY3M_004009"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFS4318830.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7739..9283)
                     /gene="malF"
                     /locus_tag="ACHY3M_004010"
     CDS             complement(7739..9283)
                     /gene="malF"
                     /locus_tag="ACHY3M_004010"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFS4318831.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9437..10627)
                     /gene="malE"
                     /locus_tag="ACHY3M_004011"
     CDS             complement(9437..10627)
                     /gene="malE"
                     /locus_tag="ACHY3M_004011"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFS4318832.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10992..12107
                     /gene="malK"
                     /locus_tag="ACHY3M_004012"
     CDS             10992..12107
                     /gene="malK"
                     /locus_tag="ACHY3M_004012"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFS4318833.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12179..13519
                     /gene="lamB"
                     /locus_tag="ACHY3M_004013"
     CDS             12179..13519
                     /gene="lamB"
                     /locus_tag="ACHY3M_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFS4318834.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13762..14682
                     /gene="malM"
                     /locus_tag="ACHY3M_004014"
     CDS             13762..14682
                     /gene="malM"
                     /locus_tag="ACHY3M_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFS4318835.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14911..15710
                     /locus_tag="ACHY3M_004015"
                     /pseudo
     CDS             14911..15710
                     /locus_tag="ACHY3M_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3958 a         3958 c         4077 g         3717 t
ORIGIN      
        1 aaacgccatc gctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
       61 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      121 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgc gtgggctgca
      181 cctgtacagg tggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg gggagttcct
      241 ccgctaccat cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg
      301 gcggtgatgg ttctaatacc gggaccacca caaccacgac caccagtacc cagtaataaa
      361 gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt
      421 accataacca cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc
      481 tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg
      541 caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat
      601 tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt
      661 ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct
      721 tgtcacacaa catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt
      781 gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg
      841 gacgcgcacg atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt
      901 cttcaaatgg ggtggcaccg ataccgtcaa agtcggcagc gatgaaaccc tggttcgcgt
      961 gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag
     1021 cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac
     1081 cactctcatc aaggcggcaa aacaatgatt aaacaaactt ttgtcgcgtt gcttttgagt
     1141 gtgggagcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa tggcagcagt
     1201 gaggccaaaa cactaacagg cgcagagcat ttaatcgatc tggtaggcca accgcggctg
     1261 gcaaacagct ggtggcccgg tgcggtgatt agcgaagagc tggcaacggc ggcagcattg
     1321 cgtcagcagc aggcgttgct gacacggctg gcagaacagg gcgcagattc cagcaccgac
     1381 gatgccgccg ccattaacgc cttacgccag caaatccagg cattgaaagt gacaggcagg
     1441 caaaaaatca atcttgatcc cgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg
     1501 cagggcaact acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt
     1561 atcagccatc ctggcaatca gccattcact ccaggtcgcg acgtggcgag ctatctctcc
     1621 gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt gggtcgttta cccggacgga
     1681 cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc
     1741 agcattattt atgttggcct cgcggactcc gtctggagtg agacgcctga tgcccttaac
     1801 gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa agacatctgc
     1861 ttagcttact ggcgctgggc attagcatgg cttgctacgg cgaaacatat cctgcgccca
     1921 ttggcccgtc gcagtcggat ttcggtggcg taggattatt acaaactccc accgcgcgta
     1981 tggcacggga aggggagttg agtctgaact atcgcgataa cgatcagtac cgttattact
     2041 cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca
     2101 cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa gataaagcct
     2161 tcgatctcaa actgcgtttg tgggaagaga gttattggct gccgcaagtg gcggttggtg
     2221 cgcgggatat cggcggtacg gggctgtttg atgcggaata tcttgttgcc agcaaagcct
     2281 gggggccgtt cgattttacg ctcggcctgg gctggggata tctgggcacc agcggtaatg
     2341 tgaaaaatcc gctctgttca gccagtgata aatattgcta tcgcgataac agctacaaac
     2401 aggcgggatc tatcgacggt agccagatgt tccacggtcc tgcctcactg tttggcggcg
     2461 tggaatacca gacgccctgg caaccgctgc gcctgaaact ggagtatgaa ggcaataatt
     2521 atcagcagga ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggtgcgattt
     2581 atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt
     2641 ttggcgttac gttgcgcacc aactttaacg atctgcgccc gtcttacaac gataacgccc
     2701 gcccgcaata tcaaccgcag ccgcaggatg ccattttgca gcattcggtg gtggcgaatc
     2761 agttaacgtt gctgaaatac aatgccggac ttgccgatcc gcagatccag gcgaaaggcg
     2821 atacgctgta tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc
     2881 gtgcgaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacag
     2941 aaaatcgcct taacatgccg caggtgacga cggaaaccga tgtcgccagc ttgaaaaatc
     3001 atctcgccgg ggagccgctg ggccatgaaa cgaaggtggc gcaaaaacgc gtcgagccag
     3061 tggttccgaa gtccaccgag cagggatggt atatcgacaa atcacgcttt gatttccata
     3121 tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg
     3181 gcgtgatggg aacggcagat ttgtggctga cggaccatct gctgaccacc ggcagcctgt
     3241 ttgcaaatct tgccaacaac tacgacaagt ttaactacac caatccgccg caggactcgc
     3301 acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata
     3361 acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag gtctatggag
     3421 gttatctcga aaccatgttt ggcggtgccg gggcagaagt gttgtatcgt ccgctggaca
     3481 gcaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa
     3541 aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc gcctactgga
     3601 cgccatcttt cgcccaggac gtgttggtga aagcgagcgt cgggcagtat ctggcagggg
     3661 ataaaggcgg cacgctggag atcgccaaac gctttgatag cggcgtggtg gttggcggct
     3721 atgccacgat cacgaatgtt tcgaaagagg agtacggcga aggggacttc accaaaggcg
     3781 tgtatgtctc tgtgccgttg gatctcttct cgtctggccc gacacgcagc cgtgcggcga
     3841 ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt
     3901 atgacatgac cagcgaccgt agcgtcaatt tccgctaagt catgggaaag gtgccagttt
     3961 tcgcaatcac tggcaccctc attcttaatg gcctgttgag ccatcaggaa cgcagttcac
     4021 ccccggtttg cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat
     4081 cggttgcaga tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg
     4141 cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa gaacagagac
     4201 tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt
     4261 catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct
     4321 gaatgggtga tttttgaatt accggctttg gtgcggtttg tcttgccgga tgcgccgcca
     4381 ggcgcggctt atccggccta cgggtaggta tatccggctt tggtggaggc gcgctccaaa
     4441 tccaggttga acaaaacata cacaaaaaat atagatctcc gtcacatttt tgcgttatac
     4501 aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg
     4561 agtttatctc caccattttg cagaccgtac tcaatcttgg cctgttgtgc ctcggcctga
     4621 ttttggttgt cttcctcggc aaagaaacgg tgcatctggc tgatgtgctg ttcgcgccag
     4681 aacaaaccag caaatatgag ctggtagaag ggctggtggt ttactttctc tatttcgaat
     4741 ttatcgcgct gattgtgaag tactttcagt ccggttttca cttcccgcta cgttactttg
     4801 tctatattgg gatcaccgcc attgtgcgct tgatcatcgt cgatcataaa tcgccactgg
     4861 atgtattgat ctactcggca gcgatcctgt tactggtgat caccctgtgg ctgtgtaatt
     4921 cgaaacggct gaagcgggag taaaaagaca gcacgccgaa ctggcgcggc gtgctggaca
     4981 ggaagattac agtgtagcag tttgttgtgt tttcttcgtt tccggttccc agagggcttc
     5041 cagctcctca agggttttac ctttggtttc cgggacaaat ttccacataa atagtgctgc
     5101 cagaacgccc atacaaccgt aaatccagta ggagaaaccg ttgtggaaat gggtcaccag
     5161 ccaggagttt ttgtccatca tcgggaaggt ccaggagacg aagtagttcg ccagccactg
     5221 ggccgccacc gcgattgcca gcgctttacc acgaatagca ttcgggaaga tttccgacag
     5281 cagtacccag cataccggac cccaggacat ggcaaaggca gcaacataga acagcatcga
     5341 cagtagcgcc acaatacccg gtgcctgagt gtaaaacgcg gtaccgaggc taaacatacc
     5401 gattgccatt ccgagcgcgc cgataatttg cagtggctta cgaccaaatt tatccaccgt
     5461 cataattgcc agaacggtga aggtgaggtt gataactccg acaataatgg tctgcaacag
     5521 cgcgatatcc gtgctggccc ccagcgtttt gaacacttcc ggcgcgtagt acaacaccac
     5581 attgatgccg acaaattgct ggaagatgga gagcataacg ccgataacaa tcacgcccac
     5641 gccgaacatc agcagacgac caccggtttt gcgtccatga tccagggagt gtttaatttc
     5701 ctgcaccgcc tgggctgcca aagcgttgcc cataattttg cgcaggatgc cttccgcttg
     5761 ttcttgctta ccgcgtgaca tcagccagcg agggctttcc ggcacggtat aaagcagcat
     5821 taagaacagc agcgcaggga tacattccga agcaaacata taacgccagc cgtcagtatt
     5881 cagccaggtg gcatcgccgg aacgggcaat aaaatagttt acgcagtaaa ctaaaagttg
     5941 gccgaaaata atcgcaaact ggttaaaaga gaccagcttc ccacgaatat gtgcaggagc
     6001 cagttccgca atatacattg gtgagagcat tgaggctaaa ccaacaccaa taccgccaat
     6061 aatgcgataa ataacaaatt ccgggacata acctgccaga taaacaggca ctgtgttgtc
     6121 cgggtttata gaggtaaaac caagttctgg ccaggcagaa cctacaccag aaataaaaaa
     6181 caggacagca gcaatcttaa gtgaatcacg acgaccgaag cggttactgc aataaccacc
     6241 gagggcaccg ccgatgatgc aaccaatcag agcgctggct acgcaaaacc ctaacaggga
     6301 gttggcagcg gattcactta agttttgtgg agcaacaaag acggtatgga gtgactcaac
     6361 agtaccggaa ataacggcgg tgtcgtagcc aaataataaa ccacctaatg tagcgactaa
     6421 ggtaatcgaa aatatataac tggaattata ctgggtattc attcagacct gccttagacc
     6481 attctgatct tttatcagtg cattgatgtt ggaattgtca ggcagttaag gaaattatca
     6541 ttttttacaa tgcgtttacg taatgtcttt tctgtgatct taattgtgat aattatctaa
     6601 aattgatgaa aacaatacta ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg
     6661 ggaaaacaaa taacgtaaaa ataataagct ctatgatggg aatattaacc ggcgaacgat
     6721 tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc gccgaataac agtcacaagt
     6781 tgggataacg taagttgagg gtgcagcggc ataacattgg cagaacaaca tctttaacct
     6841 ttcacaccac ctgccgtcag gccgttgacc agccagcgtt gagccagcaa gaagacgatg
     6901 gtgatcggta atgcagacat cacggcagcg gcggcaaagt caccccacag gtagttttgc
     6961 gggttgaggt attgctgcat ccccacggcc agggtgtagc tgtttacgtc acgcagtaac
     7021 agcgacgcga ccggaacttc agtaatggca gcgataaacg acaagatgaa taccaccgcc
     7081 agaatcggta ctgacagcgg caacaggaca aggcggaagg cctgccacgg tgtcgcacca
     7141 tccagcgcag ccgcttcttc cagcgaacta tcgatggttt cgaaatagcc tttgatggtc
     7201 cagacatgca gcgcaatccc acccagatac gcgaaaatta cgccgccgtg agtattcagg
     7261 ccaatgaatg gaatgtactc acccagacga tcaaacaacg catacaacgc gaccagtgaa
     7321 agtactgccg ggaacatctg gaaaatcagc attcctttca gcagcgtcgc tttgcctgga
     7381 aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga gcgccacaat gccaatcgcg
     7441 gaaatcccgg cgacctttac cgagttccac agccacagca gtaccgggaa tggcggtggc
     7501 gtaatgcgac catcagcctg ttcaacgcta aaacctaacg ccagtttcca gtgatcccag
     7561 gagatttgct ccgggatcag gctaccggtc gcgaagtttc cctgacgcag cgagatagcg
     7621 acgaccatca gcagcgggaa cataatcgcc gcgataaaaa gtagcagtag caggtgagta
     7681 ataaacaaac gtgctttttg cgatttcggt tggaccattg ccatttttgt tatctccctt
     7741 aatcaaactt cattcgcgtg gctttcaggt tcactatcgc cagcgcaccc accagcagga
     7801 agatcagcgt ggcaattgct gctgccagac cgaagtcctg acccccgccg ccttcaaaag
     7861 cgatgcggta ggtgtagtta acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa
     7921 gacgatccgg gccgccgttg gttaacagtt gaatcagcac gaagttgtta aagttaaagg
     7981 cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa
     8041 agaagttctg gaacggacct gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa
     8101 tcgctttcag caagcccatg cagaggatca tcatgtacgg ataacccagc caggtattga
     8161 cgataatcag catcgtgcgg gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa
     8221 acagcgcgct caacatcatg ttgatttcac cgaagctctg gttaaacaac cctttgaaaa
     8281 tcaagattga aatgaacgat ggcaccgcgt agggcagaat cagcaggacg cgatagaccg
     8341 ctttgccgcg caacgcttcc cactgcacca gacacgccag aaccatgccg accgccaccg
     8401 ttaaaaagac agtgatcagc gagaacacca cggtccagac gaaaatggcg aggaacggtt
     8461 tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt ccagccagta gtcacggtgt
     8521 aaccggggct tagcttttca tcgccccagt tgccgtcggc ggtaatggac tggtaaaagc
     8581 caatttggtt attcggacga tatttcacgc cgctctgatt attcgtcaac gtgccgtcac
     8641 cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg cagggagctc atcatcactt
     8701 tgttgccatc cggcagaatg gcggtaatgt cactcagcgc ctgacgattc tgggtaatca
     8761 cgcgcagatt cgcgcgttcg ccttcgggct gggtggtcgt ttctttcagt tgcagttttt
     8821 gctcgccgcc aaatttaaaa gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga
     8881 gggctagttg ccactcatcg cccgctgggt aaagaccaaa attataggtt ttgcctgctt
     8941 gccaggagcg atctagcaac acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc
     9001 tgtagttggt gaaggcaatg gcgatggtgc agaccagagg gaagaggacg aataatccca
     9061 ttccagccat tcccgggtaa acatagcgcc aggcgtaggc tttacgattg gcgaaaatat
     9121 acagccccgc tgaactcaat atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt
     9181 acattaaaac aacaaggtaa cccaccagca ggccgagcag acctaacact gaccatttca
     9241 gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc catggggttc ttcctcattc
     9301 caggacggat aaggctttca cgccttatcc gacaacaact gcctgatgcg acgctgacgc
     9361 gtcttatcag gcctacatac gtttcggttt tgtaggccgg acaaggcgtt cacgccgcat
     9421 ccggcatttc acagcattac ttggtgatac gagtctgcgc gtctttcagg gcttcatcga
     9481 cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac cagaaagcgg
     9541 acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccatg gtggctgcaa
     9601 tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt
     9661 tgtctttatt aaccgcttcc agaccttcgt cagtcagcag atagttttcg aggaactctt
     9721 tcgccagctc tttgttcgga ctggcggcgt taataccggc gctcagcacg ccaacgaacg
     9781 gtttagatgg ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg
     9841 tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg cctttattaa
     9901 aggcagcttc tgcgatggag taatcggtgt ctgcattcat gtgtttgttt ttaatcaggt
     9961 caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc cacgcccacg tctttaatgt
    10021 cgtatttgcc attttcatac ttgaacgcat aacccccgtc agcagcaatc agcggccagg
    10081 tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt acctttcgct ttcagttctt
    10141 tatccagcgc cgggatctct tcccaggttt ttggtgggtt cggcagcaga tctttgttat
    10201 aaatcagcga taacgcttca acagcgatcg ggtaagcaat cagcttgccg ttgtaacgta
    10261 cggcatccca ggtaaacgga tacagcttgt cctggaacgc tttgtccggg gtgatttcag
    10321 ccaacaggcc agattgagcg tagccaccaa aacggtcgtg cgcccagaag ataatgtcag
    10381 ggccatcgcc agttgccgca acctgaggga atttctcttc cagtttatcc ggatgctcaa
    10441 cggtgacttt aattccggta tctttctcga atttcttacc gacttcagcg agaccgttat
    10501 agcctttatc gccgttaatc cagattacca gtttaccttc ttcgattttg gcgagagccg
    10561 aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca cctgttttta
    10621 ttttcataat ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa acggactcta
    10681 gtttctttat acggcaacct ctttccatcc tccttgcccc tacgccccac cgttgctttg
    10741 tgtgatctct gttacagaat tggcggtaat gtggagatgc gcacataaaa tcgccacgat
    10801 ttttgcaagc aacatcacga aattccttac atgacctcgg tttagttcac agaagccgtg
    10861 ttctcatcct cccgcctcct cccccataaa aaagccaggg ggtggaggat ttaagccatc
    10921 tcctgatgac gcatagtcag cccatcatga atgttggtgt cgatgacagg ttgttacaaa
    10981 gggagaaggg catggcgagc gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg
    11041 tggtatcgaa agatatcaat ctcgatatcc atgaaggtga attcgtggtg tttgtcggac
    11101 cgtctggctg cggtaaatcg actttactgc gcatgattgc cgggcttgag acgatcacca
    11161 gcggcgacct gttcatcggt gagaaacgga tgaatgacac tccgccagca gaacgcggcg
    11221 ttggtatggt gtttcagtct tacgcgctct atccccacct gtcagtagca gaaaacatgt
    11281 catttggcct gaaactggct ggcgcaaaaa aagaggtgat taaccaacgc gttaaccagg
    11341 tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg ctctctggcg
    11401 gtcagcgtca gcgtgtggcg attggccgta cgctggtggc cgagccaagc gtatttttgc
    11461 tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct
    11521 cccgtctgca taaacgcctg ggccgcacaa tgatttacgt cacccacgat caggtcgaag
    11581 cgatgacgct ggccgacaaa atcgtggtgc tggacgcagg tcgcgtggcg caggttggga
    11641 aaccgctgga gctgtaccac tatccggcag accgttttgt cgccggattt atcggttcgc
    11701 caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg
    11761 agctgccgat gccaaatcgt cagcaagtct ggctgccagt tgaaagccgt gatgtccagg
    11821 ttggagccaa tatgtcgctg ggtattcgcc cggaacatct actgccgagt gatatcgctg
    11881 acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa acccaaatcc
    11941 atatccagat cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg
    12001 tagaagaagg tgccacattc gctatcggcc tgccgccaga gcgttgccat ctgttccgtg
    12061 aggatggcac tgcatgtcgt cgactgcata aggagccggg cgtttaagca ccccacaaaa
    12121 cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag gagatagaat
    12181 gatgattact ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc
    12241 tcaggcaatg gctgttgatt tccacggcta tgcacgttcc ggtattggct ggacaggtag
    12301 cggcggtgaa caacagtgtt tccagactac cggtgctcaa agtaaatacc gtcttggcaa
    12361 cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa gtgtggaaag agggcgataa
    12421 gagcttctat ttcgacacta acgtggccta ttccgtcgcg caacagaatg actgggaagc
    12481 taccgatccg gccttccgtg aagcaaacgt gcagggtaaa aacctgatcg aatggctgcc
    12541 aggttccacc atctgggcag gtaagcgctt ctaccaacgt catgacgttc atatgatcga
    12601 cttctactac tgggatattt ctggtcctgg tgccggtctg gaaaacatcg atgttggctt
    12661 cggtaaactc tctctggcag caacccgctc ctctgaagct ggtggttctt cctcttttgc
    12721 cagcaacaat atttatgact ataccaacga aaccgcgaac gacgttttcg atgtgcgttt
    12781 agcgcagatg gaaatcaacc cgggcggcac attagaactg ggtgtcgact acggtcgtgc
    12841 caacctgcgt gataactatc gtctggttga tggcgcatcg aaagacggct ggttattcac
    12901 tgctgaacat actcagagtg tcctgaaggg ctttaacaag tttgttgttc agtacgctac
    12961 tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg cgtttgataa
    13021 cgaaaaattt gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg
    13081 tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa
    13141 ctgggataac gacaacggca ccaagtggtg gaccgttggt attcgcccga tgtacaagtg
    13201 gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac
    13261 cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag
    13321 catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg
    13381 gggttatgac tacaacggcg atagcaaggt taacccgaac tacggcaaag ccgttcctgc
    13441 tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca
    13501 gatggaaatc tggtggtaat agcaaaacct gggccggata aggcgtttac gccgcattcg
    13561 gcaaccaacg cctgatgcga cgcttgcgcg tcttatcagg ccgacaacgg ctgtcaaatg
    13621 taggccggat aaggcgttta cgccgcatcc ggcataaaaa caggttgtca ttatctgaaa
    13681 ggggcgaaag cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct
    13741 ccagattttg aggtgaaaac aatgaaaatg aataaaagtc tcatcgccct ctgtttatca
    13801 gcagggttac tggcaagcgc gcctggaatc agccttgccg atgttaacta cgtaccgcaa
    13861 aacaccagcg acgcgccagc cattccatct gctgcgctgc aacaactcac ctggacaccg
    13921 gtcgatcaat ctaaaaccca gaccacccaa ctggcgaccg gcggccaaca actgaacgtt
    13981 cccggcatca gtggtccggt tgctgcgtac agcgtcccgg caaacattgg cgaactgacc
    14041 ctgacgctga ccagcgaagt gaacaaacaa accagcgttt ttgcgccgaa cgtgctgatt
    14101 cttgatcaga acatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa
    14161 ccaggcgtga tgagtgcaga tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg
    14221 cagcaaaaac tttatgttct ggtctttacc acggaaaaag atctccagca gacgacccaa
    14281 ctgctcgacc cggctaaagc ctatgccaag ggcgtcggta actcgatccc ggatatcccc
    14341 gatccggttg ctcgtcatac caccgatggc ttactgaaac tgaaagtgaa aacgaactcc
    14401 agctccagcg tgttggtagg acctttattt ggttcttccg ctccagctcc ggttacggta
    14461 ggtaacacgg cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag
    14521 ccgatgctca acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa
    14581 ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg aacgcctggg atcgacatct
    14641 gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg
    14701 attttgcaac aactggtgcg tctcctggcg cacctttttt tatgcttcct tcctgggata
    14761 tgagcgattt tttatagtaa ctcacttctt cttcactaag aatatccatt atctcaatgc
    14821 cttatcagag attcttttcc tttcgccggt agtgtctgga cattcaggct acttttccag
    14881 gttattttat ttctgttatg cagaggtttt atgataagtc atatcctaaa ttctggcggc
    14941 aataactctt tgatgaaaca tgatgtggtg caaggaaata atatagtaga tcttgattta
    15001 ctacgtaatt tcaatggggt gccaggttta aacagagata actttattta tatcagcaat
    15061 attttttcaa atataaaaca acggaacgaa aaaatcatgc aataaatttg tttcgtgaag
    15121 tctcaatcag taatgatact ataagtgtaa aattctacag aaatgaaaaa aattgaatgc
    15181 gcttgcaatt ttctgatgga taaagatgcg caggggtata tcgacctgtc tgatttggat
    15241 ttaacaagtt gtcattttaa aggtgacgtt atatcgaagg tgtctttttt atcatcaaat
    15301 ctacaacatg taacattcga atgtaaagaa attggggatt gcaattttac tactgcaata
    15361 gttgataatg tcatatttag atgtcgacgt ttacacaatg tgatttttat caaagcgagt
    15421 ggtgaatgtg tcgatttcag caaaaatatt cttgatacag ttgacttctc gcagagtcaa
    15481 cttactcaga gtaattttcg cgaatgtcag attagaaatt caaacttcga taattgttat
    15541 ctttacgctt cgcacttcac cagagcagag tttctgtctg ccaaagaagt atcatttatt
    15601 aaatcgaata tgacagctgt tatgtttgat catgtgcgaa tatcgacagg gaattttaaa
    15661 gattgcatta cagaacaatt ggagttaact attgattatt cagatatatt
//
LOCUS       DAZUFB010000114        15576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZUFB010000114 DAZUFB010000000
VERSION     DAZUFB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.114"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..645
                     /gene="yceJ"
                     /locus_tag="ACHY3M_004016"
     CDS             79..645
                     /gene="yceJ"
                     /locus_tag="ACHY3M_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS4318836.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPAPLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            649..1224
                     /gene="yceI"
                     /locus_tag="ACHY3M_004017"
     CDS             649..1224
                     /gene="yceI"
                     /locus_tag="ACHY3M_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFS4318837.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1266..2318)
                     /gene="trhO"
                     /locus_tag="ACHY3M_004018"
     CDS             complement(1266..2318)
                     /gene="trhO"
                     /locus_tag="ACHY3M_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4318838.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2543..3463
                     /gene="lpxL"
                     /locus_tag="ACHY3M_004019"
     CDS             2543..3463
                     /gene="lpxL"
                     /locus_tag="ACHY3M_004019"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFS4318839.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3635..4861
                     /gene="mdtG"
                     /locus_tag="ACHY3M_004020"
     CDS             3635..4861
                     /gene="mdtG"
                     /locus_tag="ACHY3M_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFS4318840.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4944..5318
                     /gene="msyB"
                     /locus_tag="ACHY3M_004021"
     CDS             4944..5318
                     /gene="msyB"
                     /locus_tag="ACHY3M_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFS4318841.1"
                     /translation="MTMYATLEEAIDAAREEFLADTPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5319..5546)
                     /gene="yceK"
                     /locus_tag="ACHY3M_004022"
     CDS             complement(5319..5546)
                     /gene="yceK"
                     /locus_tag="ACHY3M_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS4318842.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5719..8262)
                     /gene="mdoH"
                     /locus_tag="ACHY3M_004023"
     CDS             complement(5719..8262)
                     /gene="mdoH"
                     /locus_tag="ACHY3M_004023"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFS4318843.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8255..9790)
                     /gene="mdoG"
                     /locus_tag="ACHY3M_004024"
     CDS             complement(8255..9790)
                     /gene="mdoG"
                     /locus_tag="ACHY3M_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFS4318844.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10184..11341
                     /gene="mdoC"
                     /locus_tag="ACHY3M_004025"
     CDS             10184..11341
                     /gene="mdoC"
                     /locus_tag="ACHY3M_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFS4318845.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11349..12830)
                     /gene="clsC"
                     /locus_tag="ACHY3M_004026"
     CDS             complement(11349..12830)
                     /gene="clsC"
                     /locus_tag="ACHY3M_004026"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFS4318846.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12772..13305)
                     /gene="ymdB"
                     /locus_tag="ACHY3M_004027"
     CDS             complement(12772..13305)
                     /gene="ymdB"
                     /locus_tag="ACHY3M_004027"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFS4318847.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13400..13711)
                     /gene="ymdA"
                     /locus_tag="ACHY3M_004028"
     CDS             complement(13400..13711)
                     /gene="ymdA"
                     /locus_tag="ACHY3M_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFS4318848.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13832..14164)
                     /gene="csgC"
                     /locus_tag="ACHY3M_004029"
     CDS             complement(13832..14164)
                     /gene="csgC"
                     /locus_tag="ACHY3M_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFS4318849.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14223..14678)
                     /gene="csgA"
                     /locus_tag="ACHY3M_004030"
     CDS             complement(14223..14678)
                     /gene="csgA"
                     /locus_tag="ACHY3M_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFS4318850.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14719..15174)
                     /gene="csgB"
                     /locus_tag="ACHY3M_004031"
     CDS             complement(14719..15174)
                     /gene="csgB"
                     /locus_tag="ACHY3M_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFS4318851.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3871 a         3998 c         3701 g         4006 t
ORIGIN      
        1 aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa
       61 ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct
      121 gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt aggtctgtgg
      181 atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc
      241 atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca
      301 ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa ggctggacat
      361 ctcgcattgt acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc
      421 gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgcccct tgccgacgct
      481 ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta
      541 ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact
      601 ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc
      661 ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat
      721 tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcatcca gcatcttggc
      781 tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt tgacgaaaaa
      841 aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaatcac
      901 gccgaacgcg ataaacatct tcgcagtgca gatttcctca ataccgcaaa atatccacag
      961 gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat
     1021 ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc
     1081 gacgacccat ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag
     1141 gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca
     1201 gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc atccggcaat
     1261 gatatttatt ccgttggatc aggaatgccc agtgttgtat tcagacgtcc acgagactta
     1321 ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt
     1381 aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc cgcacatact
     1441 ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac
     1501 ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc
     1561 tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta
     1621 cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc gttatgtttc
     1681 atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac gattttttta
     1741 tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc
     1801 ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt acgcatgtcg
     1861 ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg cagatactca
     1921 ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt
     1981 accttcatgc gcagtaccca gaaagatttc ccgtcatcat ccaacgcgat attcaggcgt
     2041 aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc aacattgctc
     2101 gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg cccaaaaaca
     2161 ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg
     2221 aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat tttggctttt
     2281 agcgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc ctgcgagatg
     2341 ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc cacacatttt
     2401 acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat ttcgcccagt
     2461 cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca attgcccgaa
     2521 taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg cactgcttca
     2581 tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg tgcaattgcc
     2641 ctacccggtt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac gttttatgaa
     2701 acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca
     2761 agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc tgatggaaac
     2821 cggcatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag tgatcggcat
     2881 ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg gcatccattt
     2941 tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggga ttggcgttta
     3001 ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt tgcgctcaaa
     3061 taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga aaaaaggcga
     3121 agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt
     3181 atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac ggatgtccgg
     3241 cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt atcaattgat
     3301 tatgctgccg ccagagtgtt ctccgccact ggatgatgcc gaaactaccg ccgcgtggat
     3361 gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt ggttacaccg
     3421 tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc ctgccggatg
     3481 cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga taaaagctct
     3541 ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt tcttcttatg
     3601 ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa atgacacccc
     3661 tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta ccggtgccgc
     3721 cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggcg ttaccggtca
     3781 ctccgccctg aatatgtggt ccggtattgt cttcagcatt acatttttat tttcggccat
     3841 cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc cgaaaactca tgctattacg
     3901 ctctgccctc ggcatgggca tcgtgatggt gttgatggga ctggcacaaa atatctggca
     3961 gtttttgatc ctacgggcgc ttcttgggtt acttggcgga tttgtcccca acgctaatgc
     4021 tcttatagcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta cgctctccac
     4081 aggcggcgtt agtggtgcgt tgctcggccc aatggctggc ggcctgctcg ccgatagcta
     4141 cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct ttttcgtcac
     4201 cctgttctgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc tgcacatgcg
     4261 ggaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg tcaccacgtt
     4321 aatcatccag gtggcgacag gctcaattgc ccccattctg acgctgtatg tccgcgaact
     4381 ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg tgccaggcgt
     4441 ggcggctctg ctaagcgcac cacgactcgg caaacttggc gatcgaatcg gacccgaaaa
     4501 gatcctgatt acagcgctga tcttttctgt actgctgttg atcccaatgt cttacgttca
     4561 gacgccattg caacttggga ttttacgttt tttgctcggt gccgccgatg gtgcactact
     4621 ccccgccgta cagacactgt tggtttacaa ctcgagcaac cagatcgccg ggcgtatctt
     4681 cagctataac caatcgtttc gtgatattgg caacgttacc ggaccattga tgggagcagc
     4741 gatttcagcg aactacggtt tcagagcggt atttctcgtc accgctggcg tagtgttatt
     4801 caacgcagtc tattcatgga acagtctacg tcgtcgtcga ataccccagg tttcgaactg
     4861 atttttcgcc tttcatactt gcaaaagcgg agaatcagct atccttttcc ctgaaacctc
     4921 atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa gccattgacg
     4981 ctgcacgcga agaatttctt gcagacaccc ccggcatcga cgccgaagat gcgaatgtgc
     5041 aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg caagttgagt
     5101 tttttgccga cgaaggggaa gaaggtgaat gtttacctat gcttagcggt gaagccgcgc
     5161 aaagtgtttt tgatggcgac tatgatgaga tagagatacg ccaggagtgg caggaagaga
     5221 atacattaca tgaatgggac gagggggaat ttcagcttga gccaccgctg gataccgagg
     5281 aaggacgcgc agcagctgat gagtgggatg aacgttaatc actcatacgg gccatgatga
     5341 atgtcgatcg gcagcagtaa agtatcaaaa accagagaga atggcagatc aaggatcgtg
     5401 acataacgcc aggcggagtc acgcacatcc cattgcacac cgggataata ttggttgcca
     5461 tgcccctgcc ccggtatagt gcgactaata atgctgccac agccactcag caaggtcacc
     5521 atgatgctca ccacaattaa acgcatttga tacctctttc gtaaaaaaat accggcataa
     5581 cgccggtatt tttattggct cgttgttttt gccggatgcg gcgtgaacgc cttatccgac
     5641 ccacaaaatc ttgcaaaatc aataaattgc aggaacgatg taggcctgat aagcgtagcg
     5701 catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg ccagtggatt
     5761 gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctccgggg aattccagac
     5821 gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc gacgatcgcg
     5881 attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac gggcgatttc
     5941 cagcacctta ctggcgcggt gacgcgcggt cgccattgcg gttgccagag cgttaaatga
     6001 cgggttaaac actgcgtgca taaagccatc atcaagggag cgttgacgat tcatctcaag
     6061 gaaccgatcg gtatcaacca gcacctgcgg cggcgaatac tcttccggga tcaggaacag
     6121 tttccagcgt ttggtgcgca gaccaacggt ggcacggctg gaaatcaccg aaacaaacgg
     6181 tgacaggatc aacgagaaga caatcggtgc cagccagaac aggaaacgca gatccaacca
     6241 cgccatacca acagcccaca ctaaccctaa cagcagctgt gagccgtggc gtttgaacgc
     6301 ttcaccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca cttcccagcc
     6361 aaggaacgcg ctgacgacga agaccgtatg gaacagcatg cgtaccggag ccagcagcac
     6421 ggaaaacagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc cgtattcttt
     6481 cgttcctttg caccagataa gcaaaatgct caataacttc ggcaggaaca acagcaccat
     6541 ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccaca ccgggaacaa
     6601 ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgtactacct gcaatgcagt
     6661 agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca cgcccgtcag
     6721 gaataccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga agttcatcag
     6781 gttaccgtgg caccagcggc ggtcacgttt tagctcatca agcaagttag gcggcaattc
     6841 ttcataagaa cccgggagat cgtaagcaat ccagaccccc caacctgcac ggcgcatcaa
     6901 cgccgcttcc acgaagtcat gtgacaggat tgaaccggca aaggaacctt cgcccggcag
     6961 cggagccagt gcgcagtgct cgataaacgg tttcacgcgg ataatcgcgt tatgtcccca
     7021 gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg gcccatacac
     7081 gcgggtcgcg aactgctgac agcgcgcata cagcgtatcc ataccggacg ctttcggcga
     7141 cgactgaatg atcccggcgt tcgggttggc ttccatcagg cgcaccaggc cgcataaaca
     7201 atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact ggctgcccca
     7261 gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcggcgac ggcggcggcg
     7321 atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg ctttttgctc
     7381 tgcgacgcag atatccgggt tataactgtc actaagaatg tagacatcaa agtgtttggc
     7441 attcccggtg gcttttactg attcccacgt tgcacgcagg ccagcaaaaa cacggttcac
     7501 gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta atggttcatc
     7561 gccaactgtt gacgcagata tactgtattt atcgcgacca ataagcagtt gcaggaagcc
     7621 cattaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa agaggatcag
     7681 gataccggtt tgcagcatat aaggcagaag ctgcataaag gaaacccaca aatcctgacc
     7741 aaccatatcc ataggattaa tcagcgccca accctgataa ggaagaatgg tcttcatata
     7801 ccaggtcgcg acgacagttt gcgcgagagt caggatcaac agaatgtaac ggcggatggt
     7861 accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac gagccagata
     7921 gcgcggcgtg acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg ggttggtacg
     7981 ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttca gctgatcgcg
     8041 cccttcgtcg tctttaatta actgtccatc agcaagtgaa tctggccagg cttgttccag
     8101 acgcgccttt accgagcctt gcggggaatc atcctcccgc gcccaggtgc ggtgttcggc
     8161 atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt tctcgcttgc
     8221 ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt aactggtagc
     8281 tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt tcagtggttt
     8341 tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta accgggttat
     8401 agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc gctgtgaccg
     8461 gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca aaggcgatag
     8521 taccgtcagg ctggcgaatc aggttcgact gcttcacatc ccccgttgaa cgacgcgttt
     8581 gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg aaggtgatgg
     8641 tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc cagtaagcga
     8701 cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg ctgcctttac
     8761 cccactcccc tttcggagtc acccatgcgc ttggacgaag atcgtaacga tcatcgagat
     8821 cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc gaagccttgc gggttctcca
     8881 tcgagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag atccattcgc
     8941 cattaccggc atggatagac agaccgttag agtcgtgcaa ctccggacga tagttatttg
     9001 caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca acccccagtt
     9061 tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg tcacgccctg
     9121 gcataaccac gaatttgtaa gcacctgtcg cgcgcggcga gtcaagcaat gcataaatgg
     9181 ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag cgtggaaatt
     9241 cttcacccga tggcaaggcg gtatcaattg ccaggccgcg ggcagaaagg ccataaacct
     9301 gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt tcatcgtttt
     9361 tatctttgct gttgatcggg taaagcactt taaaaccggc aaaaccaagg tcttttaccg
     9421 tgtctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat ttgattcgtt
     9481 tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac ataccctgat
     9541 ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg tcatgattaa
     9601 actggatctg ctgatagtcc gcgtatttca tatcgcggaa aacggagggc aagttgcttt
     9661 tgggcgcctc atagcctttc ccggctaagg attgagcttg ctttgcgaca tcatcaatac
     9721 tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc aaccaacgca
     9781 ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt tgtgtgctta
     9841 tatcgatccg atctatttta atggataacc agccattccg acaacagaat cccggtatcg
     9901 ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact ttccgtgctt
     9961 ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaatttta tttatcaacc
    10021 gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat aaaaaccttg
    10081 aagcctgcgc ctggcgcggt caggtcggtg tcgctatggc aacaagccta aaggcataac
    10141 ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaatc cagtacccgc
    10201 gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag ggatcccttt
    10261 tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg aaccatcatt
    10321 gtggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat ttttcgttat
    10381 atccggctac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat ggtggaaagt
    10441 acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga cattaccgca
    10501 atttattatg ctgcaatatg tcaaaggaaa agcggaaagt tggcctgggc tgtcattgta
    10561 tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt tactggtgtt
    10621 agtagtcatg acgacgctgt gcgtatggat atttaagcgc atcagaaata atttagaaaa
    10681 ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt ttttatgcct
    10741 cggcatcggt tatgcggtaa taagaagaac gatttttatt gtgtatccgc ccattctgag
    10801 taatggcatg ttcaatttta ttgtcatgca aacgctgttt tatttgccgt tctttatcct
    10861 cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc cgtctcgtgg
    10921 ctgtaccctt gcagcagcat tggcgtttgt cgcttattta ctcaaccagc gctatggcag
    10981 tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg gtctgtggat
    11041 ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac ttccagtcag cgcgggtgac
    11101 ttattttgtt aacgcatcgc tgtttatcta tctggttcac cacccgttaa cgctgttttt
    11161 cggcgcatac attacaccgc acatcacctc caactggctt ggttttctct gtggcctgat
    11221 atttgtagta gggattgcga taattctgta tgaaattcat ttgcgcatcc cgttactgaa
    11281 gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac cagcccgtta
    11341 agccacattt acaataacca ttccacgggc aatatcgacg ccagtctgac cataacccgc
    11401 ttccagaaac tggtggcggg ttcttttttg agaataatct ctttctttgc atgacgatca
    11461 acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc atactggctc
    11521 tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac gaagcccatt
    11581 tcagtattga gcaatgttga acgcggatcg aaattgaaag agccgataaa caccgtttta
    11641 ccatcgatgc taaaggtttt agcatgcagg ctggctccgg aattaccggt tatgccgcga
    11701 tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc cacgccatag
    11761 cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac atcgttagcg
    11821 gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg taagagttgc
    11881 gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg ttcactgggt
    11941 gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc cttgccctcc
    12001 cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg caacgttcca
    12061 tcaaccagat gatttataaa cggactggat tccattttgc gtaagtaacg atgcgtcata
    12121 gcatcgttat gccaggaggc gggtaactcg atgcgatccg ccatttcacc ttccgggaca
    12181 tccagcactt gctgtaaggg tgaaaccgat ttgcaatacc agtagcgggc gaaatcatcg
    12241 gcaacgtcct ctaccactgg ccctattgcc ataacatcta aatccgaaaa aagtggctct
    12301 tcccctgccc caaaataggc atcaccaata tttcgccccc ctaccagcgt caccacgcca
    12361 tcgacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc ggtgatataa
    12421 ccaagcggac gcagcaagcg aaacgagaaa ggattaaaaa gccggacttc aatgcgggga
    12481 tggctgtcaa gcaagcgtaa aatgtcgtca agtccgggcg tattgttgtc gtccagcaac
    12541 aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaacag taaccgaccc
    12601 gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc catttcggcc
    12661 agacgatacc gggcggcaaa cgcatccaga cttttctcca gagggaaaag accacactga
    12721 ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa atcattcatc
    12781 tccttgttgg gtaaggagtc tttcgtagag gtgggcgttt tcttcatcat aacagacaaa
    12841 gtatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt taactgcgat
    12901 ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag gaaaagccac
    12961 tgacgtatag ctgtttgccg ccactagtcg taggctattg agataggcat cctgcaaaag
    13021 ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca ctacggcttt
    13081 agcgggaaga gcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat cgccctgctg
    13141 ttgcctgact tttaaacagg catccagcag ggccggaccc gctgcgcgat gaatggcccc
    13201 atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca catcaacggc
    13261 cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc ctccttacac
    13321 cttcttgttt cttaagtgta cagcaggctt tttggctaaa tcgcattttt ctgctcagca
    13381 acgtgcgttg ttcagaaact tagttatatt cgatgttcat tacagccagc tttttctgtt
    13441 tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttaacatta ccgctcatta
    13501 acgctttgct ggaataggtt ttgcccggaa cagaaccatt ctgtgggcag ttcatttcta
    13561 cgggtgatga ctgggtgctg acgtcacatg ccggttccac aatttggcca taaaaatgga
    13621 tcaccccccc ttgcgcggtg cttcctgcga tggcaataaa aggaaaaagc aaactgccga
    13681 gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg attagtcatc
    13741 cttgagggtt gtgttatcca tactttcagg ttggctgatt cgcagcagac cattctctcc
    13801 agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag ggcggccatt
    13861 gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc acccgatctt
    13921 ccggggaaat atttaaactc aacttcgtca aagcaatagg ttgattagca ggcaatgaga
    13981 gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg gacaatattt
    14041 gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggta tacacatccc
    14101 cttgctgggt cgtattaaag gttatctggc tggaaagtgc cgcaaggagt aataacgcat
    14161 tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctgtaat acaaatgatg
    14221 tattagtact gatgagcggt cgcgttgtta ccaaagccaa cctgagtcac gttgacggag
    14281 gagttagatg cagtctggtc aactgcagca ccgttgccac caccgaactg tttaacagtc
    14341 atttcagaat ttttgccgtt ccactgatca agagtggcgc tgttaccgaa gccacgttgg
    14401 gtcagatcga ttgagctgtc atctgagccc tgacctacat ctgcaccatt accaccgcca
    14461 tgctgggtaa tagtcaagtc agagttacgg gcatcagttt gcagagcaag tgcagagtta
    14521 ccgccaccgt actggtaaat gttcagctct gaatttgggc cgctattatt accgccacca
    14581 ccgtggttac cgccgccgcc gtactgagga acaacacctg ccagagcgct accggagaat
    14641 acgattgctg caattgctgc tacttttaaa agtttcatgt aaaaccccca tcggattgat
    14701 ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata gccatttgcg actgtctctg
    14761 cactacaatt gccgtttttt gagtaccata ctgtgtaata tttgctttat taccagaacc
    14821 tttctggata atcatcgcag tattaccata agcaccttgc gaaatactgg catcgttggc
    14881 actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa tctttgcccg
    14941 gttgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct gccgtaactg
    15001 agcactatta ttcgtcccag cttgaccaat tatggctgcc tgattaaatg aagacttact
    15061 caattcattt accgcgaagt tatattctga attagctaaa tcataacctg ctgcggctgc
    15121 aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt tcatgttgtc
    15181 accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt gttaacgcaa
    15241 cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat atttttaata
    15301 actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa aacccacgta
    15361 aatacgctgt tatctacgca aaaaatattt tgttttcttt taaatctccg ttttccgcta
    15421 tcaaaaatca ccagacagtc atttttcttg cccgtcgctg attgctgcga tatgtcttgc
    15481 gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg aggtgcggag
    15541 aaaaaacaag aacgttttac atgacgaaag gactac
//
LOCUS       DAZUFB010000115        15053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZUFB010000115 DAZUFB010000000
VERSION     DAZUFB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.115"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(79..2358)
                     /gene="maeB"
                     /locus_tag="ACHY3M_004032"
     CDS             complement(79..2358)
                     /gene="maeB"
                     /locus_tag="ACHY3M_004032"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFS4318852.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEIVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2647..3597
                     /gene="tal"
                     /locus_tag="ACHY3M_004033"
     CDS             2647..3597
                     /gene="tal"
                     /locus_tag="ACHY3M_004033"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS4318853.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3617..5620
                     /gene="tkt"
                     /locus_tag="ACHY3M_004034"
     CDS             3617..5620
                     /gene="tkt"
                     /locus_tag="ACHY3M_004034"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4318854.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5716..6759)
                     /gene="ypfG"
                     /locus_tag="ACHY3M_004035"
     CDS             complement(5716..6759)
                     /gene="ypfG"
                     /locus_tag="ACHY3M_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFS4318855.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6885..7460)
                     /gene="nudK"
                     /locus_tag="ACHY3M_004036"
     CDS             complement(6885..7460)
                     /gene="nudK"
                     /locus_tag="ACHY3M_004036"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFS4318856.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7528..9507)
                     /gene="aegA"
                     /locus_tag="ACHY3M_004037"
     CDS             complement(7528..9507)
                     /gene="aegA"
                     /locus_tag="ACHY3M_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFS4318857.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9713..11413
                     /gene="narQ"
                     /locus_tag="ACHY3M_004038"
     CDS             9713..11413
                     /gene="narQ"
                     /locus_tag="ACHY3M_004038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFS4318858.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11577..14690
                     /gene="acrD"
                     /locus_tag="ACHY3M_004039"
     CDS             11577..14690
                     /gene="acrD"
                     /locus_tag="ACHY3M_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFS4318859.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14789..14848)
                     /gene="ypfM"
                     /locus_tag="ACHY3M_004040"
     CDS             complement(14789..14848)
                     /gene="ypfM"
                     /locus_tag="ACHY3M_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFS4318860.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3542 a         3987 c         3920 g         3604 t
ORIGIN      
        1 gtaataagct ttactaatat attgcagcca aaccgcttac cctgaatatt cagggtaagc
       61 gtgagagtta aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg ccagcgccac
      121 catgttgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg gtttcgcaac
      181 gcccatcagc accggaccaa cagtcacacc ttccgagctg gaaacgcgca gtaagttgta
      241 actaatgcgg gcagcttcca tgttcggcat caccagaata ttggcagaac ctttcaaagg
      301 gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc
      361 accatcaatc atcagatctg gcgcacgatc cctgaccagt tccagcgcct gacgcatttt
      421 gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag caacgcgcgg
      481 ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg ccagctcttc
      541 tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac tcggcagcag
      601 cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac
      661 cacgctaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat cggcttcccc
      721 acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgccc gctgcgcctg
      781 ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa
      841 gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgctttga tctgcaagcc
      901 cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg gtttcgccag
      961 tcccagcgtt accagttcct gagtggcatg cagaacgcgc gcctcttccc cttctggcag
     1021 aacaacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt
     1081 tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt
     1141 cgccacgccc gactccatcg cggctttagc gaccgcagga gcgatcttaa cgatcaagcg
     1201 cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct gatcgccata
     1261 cgctgaagcc accacttcgc tctgttcggc gtgggccagt tccgcaatcg cgcgtaccgc
     1321 cgccagtttc atctcttcat tgatagccgt tgcgccaaca tccagcgcgc cacggaagat
     1381 aaacgggaag caaaggacgt tgttgacctg gtttggatag tcagaacgac cggtgcaaat
     1441 gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc
     1501 cagcgccagg atcattggcg cacgagccat tttcttcact atttcctggg tcagcacttt
     1561 cgggccggaa catccaagga aaatatccgc gccttcaatc acatcatcga gggtacgttt
     1621 gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atgtttggct cacggccctg
     1681 atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac ccagcgctac
     1741 cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc
     1801 ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc tgatgattgc
     1861 cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc
     1921 aatatagaaa cattctggcg ctttaatgtc ttcgaggttg attccgccga aggtcggttc
     1981 gagcgcagcg acaacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa
     2041 tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca ccggtttgcc
     2101 agccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgccac
     2161 caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga tttcaagaca
     2221 aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt
     2281 ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg
     2341 ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg
     2401 ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt ctacagactt
     2461 tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct ataacactga
     2521 tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag
     2581 cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta
     2641 tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagcggc
     2701 gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc ttcgctgtta
     2761 ctcaaagctg ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt
     2821 aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcagtc
     2881 aatttcggtg ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga agttgatgca
     2941 cgcctctctt ttgataaaga aaagagtatt gaaaaggcgc gccatctggt ggacttgtat
     3001 cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac ctgggaagga
     3061 attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac gctgctgttt
     3121 tcttttgccc aggcgcgggc ctgtgcagaa gcgggcgttt ttctgatttc gccgtttgtc
     3181 gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa
     3241 gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca ccactatgaa
     3301 accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc
     3361 gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt ttcgccagtg
     3421 gtacgtaaat taatcccgcc ttctcagacg ttcccacgcc cagcacccat gagcgaagcg
     3481 gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt
     3541 cgtctgttcg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc
     3601 agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat
     3661 ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat
     3721 tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa cctggtatga
     3781 tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctaca
     3841 tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt tgcattcgaa
     3901 aaccccaggc cacccggaga ttggctatac gccaggcgtt gaaaccacca ccggcccgct
     3961 tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacac tggcggcgca
     4021 gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta tgggcgacgg
     4081 ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc tgggactggg
     4141 caagctgatt ggtttttacg atcacaacgg tatttccatc gacggtgaaa cagaaggctg
     4201 gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga tccatgaaat
     4261 cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga
     4321 taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga ataaagcagg
     4381 taaggaagag gcgcacggcg caccactggg ggaagaagaa gtggcgctgg cacggcaaaa
     4441 actgggctgg caccatccgc catttgagat ccctaaaaag atttatcacg cctgggatgc
     4501 ccgcgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg cctataaaaa
     4561 ggctcatccg caactggcag aagagtttac ccgtcggatg agcggtggtt taccgaagga
     4621 ctgggagaaa acgactcaga aatatatcaa tgagttgcag gcgaatccgg cgaaaatcgc
     4681 cacccgtaag gcttcgcaaa atacgcttaa cgcttacgga ccgatgctac cggagctgct
     4741 cggaggttca gcggatctgg ctcccagcaa cctcaccatc tggaaaggct ctgtgtcgct
     4801 gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg gcatgaccgc
     4861 tatcgccaac ggcattgcgc accacggcgg ctttgtgccg tataccgcga cgttcctgat
     4921 gtttgttgaa tacgcccgta atgccgcgcg gatggcggca ctgatgaaag cacggcagat
     4981 tatggtttat acccacgatt ctattggctt aggggaagat ggtccgacgc accaggccgt
     5041 tgagcaactg gccagtctgc gcttaacgcc gaatttcagc acctggcgac cgtgcgatca
     5101 ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacgggc cgacagcact
     5161 gatcctctca aggcagaatc tggcccaggt ggaacgtacg ccggatcagg ttaaagagat
     5221 tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta ttctgattgc
     5281 caccggttca gagatggaaa ttaccctgca agcggcagag aaattagcag gagaaggtcg
     5341 caatgtacgc gtagtttccc tgccctcgac cgatattttc gacgcccagg atgaggaata
     5401 tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag caggtattgc
     5461 cgattactgg tacaagtatg ttggtctgaa aggggtaatt gtcgggatga cgggttacgg
     5521 ggaatctgct ccggcggata agctgttccc gttctttggc tttaccgccg agaatattgt
     5581 ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc cggatgctga
     5641 ttgccggatg cgacgctgac gcgtcttatc cggcctacag cgtcttatcc ggcctacatg
     5701 tccccgcaat tttgtttaac gggtgatcca caacgtgggc caggcgtctg gcccatgcca
     5761 gttatcgcag gtgggttctg cggcataacg caccaggcga aaacgctgac cgtcaaagcg
     5821 ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc
     5881 acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctgaccgtt
     5941 gttgaatggc aaacgcaacc gaaccgggcg cgaggctagt ggctttttac gcgacacaat
     6001 ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagcgcttt
     6061 atcatcagtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag
     6121 accattcatc cgccagtttc cataatccag caaatcgttg cgttcttcga gtgagagtgg
     6181 cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag
     6241 cggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc
     6301 atcaataaac aacaacgccg cttttaagcc actcagagaa atggtctgat tgccgtcgcg
     6361 taaggtgatc gccttccctt cctgaatcat ctgcaaaaac gcggtgatgg ttgccgtatc
     6421 atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaacg ccaacggctc
     6481 accatctaac agcagccgtg gcgctatctc tccttcagac gcatccggcg acttcaaccc
     6541 gccgcgctca atacgtaaaa cggcatcggt atgcgccccg gcgctgcggc tcagggtcat
     6601 caccagtcca ttatgatcgc ccgtattgcg cgccacgcag aaattttggt tattacaggt
     6661 gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa
     6721 caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt
     6781 tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcagatttg
     6841 tcggattgag ccaataataa acaacgaaat attattgtta tttttcagtc cattaaatgt
     6901 gacgtttgca aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg
     6961 atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca
     7021 ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc
     7081 tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca
     7141 tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc
     7201 agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca
     7261 cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca
     7321 ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg
     7381 agatcgtagg taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg
     7441 agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgagcaatta tcttacgctg
     7501 tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca
     7561 gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc
     7621 accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg
     7681 gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag
     7741 ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac
     7801 aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag
     7861 acgcgtgcgc aggaaacgaa tcccgcagac gtgaccttgt tcattcagct caagcgccac
     7921 cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcctca cctctttctt
     7981 cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg
     8041 gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt
     8101 aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt
     8161 agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa
     8221 acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa
     8281 aagcgaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat
     8341 cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat
     8401 gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc
     8461 attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac
     8521 ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg
     8581 atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt
     8641 acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt
     8701 gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat
     8761 ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgca
     8821 cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc
     8881 atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt
     8941 tgcctgcatt tgttcgactt tactcattac cggcatttct tgcgccgcgg tactggcatg
     9001 ccacggttga tgttcctgac gcgcggtgcg caagcggcgg gatttcgcca tgccggagag
     9061 tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcaacac aggcaggacc
     9121 gttttcgcgc cccgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc
     9181 gacgggtgtc aggacgattt gcatcgtacc aaaaggacag gccaccacgc aggatttaca
     9241 gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt
     9301 agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg
     9361 ctgttgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg
     9421 ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg
     9481 actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat
     9541 tctttatatg agtgttgtta ttacccgact tacaggggaa tcggcaatgt tcatgtgccc
     9601 agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa
     9661 attgtgtttc agctaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt
     9721 taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat
     9781 tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat
     9841 caatattgcc ggatcgctac gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg
     9901 cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt
     9961 aaccaatctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa
    10021 tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc
    10081 caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc
    10141 tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac
    10201 gctggtcttt tttactttgc gccgtatacg ccatcaggtg gttgccccgc tgaatcagct
    10261 ggttaccgcc agtcaacgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag
    10321 tctgccgaat gagcttggtc tgcttgcaaa aacctttaac cagatgtcga gcgagctgca
    10381 taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgaggc
    10441 caagcgtcgt ctggaggtgt tgtatcaatg ttcccaggcg ctgaacacca gccagattga
    10501 tgtgcattgt ttccgccata ttttgcagat tgttcgcgac aatgaagcgg ctgaatatct
    10561 ggagttaaat gtcggtgaaa actggcggat tagcgaaggg caaccaaacc cggaattgcc
    10621 gatgcagatt ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa
    10681 tagtcacgtt tcatcatcag aaccgctgct taacagtgtt tcatcgatgc tgggacgcgg
    10741 tttgtacttt aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc
    10801 aaccatcgcc cgcgaattgc acgactcgct ggctcaggta ctttcttact tacgtatcca
    10861 gttgacgtta ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc
    10921 cgatttttcc caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt
    10981 ccgcctgacg ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt
    11041 acaaaatcaa accagcgcca aactgaccct cgactgccgt ctgccaaccc tggcgctgga
    11101 tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa
    11161 gcacgccaac gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac
    11221 ggtttatatc cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta
    11281 tggtctgaat atcatgcgcg aacgcgcgga acggctgggt gggacgctga ctttttcaca
    11341 accttccggc ggcggcacgt tagtgagtat tagctttcgc tctgcggagg gtgaggaaag
    11401 tcagttgatg taatgcctcc tactgaccaa agaatacttg cacttaaggt tcagtataaa
    11461 agggcatgat aatttacatt aactcctttt tttctccacg attggctcgt accttgccgc
    11521 tacagtgaag caagtcaagc ctacaacgat acgcagaaac acgaggtcct cttttaatgg
    11581 cgaatttctt tattgatcgc cccatttttg cctgggtgct ggcaatcctg ttgtgtctga
    11641 cagggaccct ggcgattttt tcattgcccg ttgaacaata ccccgatctc gcgccaccga
    11701 atgtgcgagt gaccgctaac tatcccggcg catcggccca gacgctggaa aacaccgtga
    11761 cccaggttat cgagcaaaat atgaccggcc tcgataatct catgtatatg tcatctcaga
    11821 gcagtggcac cggtcaggca tctgtcactt taagttttaa agcaggcacc gatccggacg
    11881 aagccgtgca gcaagtacaa aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg
    11941 tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac caatattctg accattgctt
    12001 tcgtctctac cgatggttcg atggataaac aggatattgc cgattatgtt gccagtaata
    12061 ttcaggaccc gttaagccgc gtgaatggcg tcggggatat cgatgcctat ggttcgcaat
    12121 attccatgcg tatctggctg gacccggcga aactcaacag tttccagatg acggctaaag
    12181 atgtcactga tgccattgag tcacagaacg cgcagattgc ggttgggcaa cttggtggta
    12241 caccttccgt cgataagcag gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa
    12301 caccagaaca gttccgcgat atcaccttgc gggtgaatca agacggctca gaggtaaggc
    12361 tgggcgatgt cgccaccgtc gaaatggggg cggagaaata cgattatctt agccgcttca
    12421 atggtaagcc agcctccggg ctgggggtaa aactggcctc cggcgcaaac gaaatggcga
    12481 cagcggagct ggtgctcaat cgtctcgacg agctggcgca gtacttcccg cacggactgg
    12541 aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga
    12601 aaacgctgct ggaagctatc gccctggttt tcctcgttat gtatctgttc ctgcaaaact
    12661 tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg
    12721 tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtactggcga
    12781 tcggtctgct ggtggatgac gcaatcgtgg tggtggaaaa cgtcgaacgt attatgagtg
    12841 aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac
    12901 tggtcgggat tgcgatggtt ctttcggcgg tatttgtacc aatggccttc ttcggcggca
    12961 ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag
    13021 tactggtagc gatgatcctc actccggctc tgtgtgccac actacttaag ccactgaaaa
    13081 aaggtgagca tcacgggcaa aaaggctttt ttgcctggtt taaccagatg tttaaccgca
    13141 acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg cgctggattg
    13201 tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt
    13261 tcttaccgct ggaagaccgt ggcatgttta ctacctcggt acaattgccc agcggttcaa
    13321 cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc acccatgaaa
    13381 aagacaacat catgtcggtg tttgccactg ttggttctgg ccccgggggt aacgggcaaa
    13441 acgtggcgcg aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca
    13501 cctcgtttgc cattatcgag cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg
    13561 ttatcgccag cagcccgcca gcaattagcg gtcttggtag ttctgcaggt tttgatatgg
    13621 agttgcagga ccacgctgga gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc
    13681 tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc ctcgacgaca
    13741 gtccgcagtt gcagattgat atcgaccagc gtaaagctca ggcgctgggc gttgctatcg
    13801 acgatattaa cgacacactg caaaccgcct ggggttcgag ctatgtgaat gactttatgg
    13861 atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc atgctgccag
    13921 atgacattaa tctctggtat gtccgaaata aagatggcgg catggtgccc ttctctgctt
    13981 tcgcgacctc acgctgggaa acaggctcgc cgcgtctgga acgctataac ggttattctg
    14041 cggttgagat tgttggggaa gccgcaccgg gggtcagtac cggtacggcg atggatatta
    14101 tggaatcgtt agtgaagcag ttgccaaacg gctttggtct ggagtggacg gcgatgtcgt
    14161 atcaggagcg actttccggc gcgcaggctc cggcgctgta cgccatttcc ttgctggtgg
    14221 tattcctgtg tctggcggcg ttgtatgaaa gctggtcggt gccgttctcg gtaatgctgg
    14281 tcgtgccgct gggggttatc ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg
    14341 acgtttactt ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa aacgccatcc
    14401 tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc
    14461 tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca tttatcttcg
    14521 gcgtattgcc aatggcaacc agcacgggtg ccggttccgg tggtcagcat gcggtgggta
    14581 ctggcgtaat gggcgggatg atttcagcta ctattctggc tatttacttc gtgccgctgt
    14641 tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa
    14701 aggcgacatg ccaatgtgtc gcctttttca actttccgat taagaacctg ctcagcgggt
    14761 tcttgctgtt tatactttgt ctcgggaatt acttacgaag cataacttcg ataaagtctt
    14821 tccagttccc cagttcacgt tcaatcataa caacctctct tataattatg ggtattctac
    14881 ggaaacaata taccgtggtg aagctaattt actcgattgc tgcgatgact acctccgggg
    14941 gacaaacctt atgtaaatac tatggtcctg cggtgataat ttgtatgtga tacacagcaa
    15001 catttcgaga tattcatacg gcatctaata ctgatttaat tctggttaaa ata
//
LOCUS       DAZUFB010000116        14963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZUFB010000116 DAZUFB010000000
VERSION     DAZUFB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.116"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..444
                     /locus_tag="ACHY3M_004041"
     CDS             184..444
                     /locus_tag="ACHY3M_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318861.1"
                     /translation="MCESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAG
                     LEDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            454..1671
                     /locus_tag="ACHY3M_004042"
     CDS             454..1671
                     /locus_tag="ACHY3M_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318862.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            1675..2403
                     /locus_tag="ACHY3M_004043"
     CDS             1675..2403
                     /locus_tag="ACHY3M_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318863.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRNKNAPETVPEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            2390..3175
                     /locus_tag="ACHY3M_004044"
     CDS             2390..3175
                     /locus_tag="ACHY3M_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HFS4318864.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRINQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            3177..4193
                     /locus_tag="ACHY3M_004045"
     CDS             3177..4193
                     /locus_tag="ACHY3M_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4318865.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSTLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            4186..4818
                     /locus_tag="ACHY3M_004046"
     CDS             4186..4818
                     /locus_tag="ACHY3M_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFS4318866.1"
                     /translation="MGSLTGKYRAVVISVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEIRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(4865..5863)
                     /locus_tag="ACHY3M_004047"
     CDS             complement(4865..5863)
                     /locus_tag="ACHY3M_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318867.1"
                     /translation="MQWNEEKPMNILIIGRKFAAISDVKTYTEMWSYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFS
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQDLFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLITIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(5863..7227)
                     /gene="dnaB"
                     /locus_tag="ACHY3M_004048"
     CDS             complement(5863..7227)
                     /gene="dnaB"
                     /locus_tag="ACHY3M_004048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4318868.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            complement(7508..7583)
                     /locus_tag="ACHY3M_004049"
     tRNA            complement(7508..7583)
                     /locus_tag="ACHY3M_004049"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7548..7550),aa:Ile,seq:cat)
     gene            complement(7611..8438)
                     /locus_tag="ACHY3M_004050"
     CDS             complement(7611..8438)
                     /locus_tag="ACHY3M_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4318869.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMDLEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            complement(9529..9604)
                     /locus_tag="ACHY3M_004051"
     tRNA            complement(9529..9604)
                     /locus_tag="ACHY3M_004051"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9569..9571),aa:Thr,seq:tgt)
     gene            complement(9609..9695)
                     /locus_tag="ACHY3M_004052"
     tRNA            complement(9609..9695)
                     /locus_tag="ACHY3M_004052"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9659..9661),aa:Tyr,seq:gta)
     gene            complement(9701..9775)
                     /locus_tag="ACHY3M_004053"
     tRNA            complement(9701..9775)
                     /locus_tag="ACHY3M_004053"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9741..9743),aa:Asn,seq:gtt)
     gene            10132..10389
                     /locus_tag="ACHY3M_004054"
     CDS             10132..10389
                     /locus_tag="ACHY3M_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318870.1"
                     /translation="MASGFLGRYHHQIIVCIYFNGQFLNRRKVANHDENPEKNAHPKM
                     GKLDARNALRRCHLGAWSTWQLGGLSGRTRPSHAYRRACRG"
     gene            complement(10448..13735)
                     /locus_tag="ACHY3M_004055"
     CDS             complement(10448..13735)
                     /locus_tag="ACHY3M_004055"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFS4318871.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAID
                     DKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRKKKEIASAIIANGAAISGESLNPCLHADMPPGMKSTKAVNA
                     FRMAAFGEYIDDETASTVKARDFKDATDLAVFSSTGAGFWSEGHGTLRAREQESHEHL
                     VTLAFPERMSGTQHAATKNTSPSLMAKNPTAVCYEVRNAEVAVRRLTPVECERLQGFP
                     EGHTLIPTEKRKKVSSDELAYLRNHYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRW
                     IGDRITKAVCRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLI
                     EPFVGGGAVFMNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGY
                     LAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEM
                     EAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQ
                     KRLVSLLTDAHRRGAKVLITNSGAPNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAH
                     DVLGILL"
     gene            complement(13732..14637)
                     /gene="rdgC"
                     /locus_tag="ACHY3M_004056"
     CDS             complement(13732..14637)
                     /gene="rdgC"
                     /locus_tag="ACHY3M_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFS4318872.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            complement(14630..>14963)
                     /locus_tag="ACHY3M_004057"
     CDS             complement(14630..>14963)
                     /locus_tag="ACHY3M_004057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318873.1"
                     /translation="YHSAIALQWAFVWSRQMQMELISRKEFDSRVTSGELDNLQAIKV
                     KEGFCLIGNQSGINRVFMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVV
                     DIQGLQHV"
BASE COUNT         3920 a         3844 c         3402 g         3797 t
ORIGIN      
        1 ttaagttaaa tattcaacaa atgactagca gtagaatcac catcatctat ttctggcagg
       61 ctgactatgg ctacatcaat cactacaacc caaagcaccc ggcaatatcc tctgtcgcgg
      121 tatgacgacc gcaacatagc cgatccaata ctcagggcag agctgcgcaa agaggtgatg
      181 cttatgtgtg aatcgaacga caagaatctg acgatttatt acgttcttcc cgatgagcaa
      241 tatcgcccgg atttgctggc ttaccgtatg tggggcatag cagagctacg ctgggttgtg
      301 acgctcgccg ccgggcttga ggatgagtct cagggtatga ctgttggcaa aaaattaaaa
      361 ctcccacctg ccacatggat ccgcgaaatg attcgccatt tccaatatga cggccaggtg
      421 atagggacat tatccattgc gtaagggaaa tgaatgccaa ctgaatatgc tcgcgacaac
      481 cttggtcgct atcagactga tggattaagt gcaaaagact ttaacaaggt attcgatctt
      541 atccgtaaac agcagcgtca gaatcggcga aacgcgcgac gtacactcac cccaaggatt
      601 atggggatgc gtaaccgcga acttgaggca ttcctcagcc ttgggaaaaa gaaagatggc
      661 acctacttta cgcccgaaga tatacgcagc ttcaacacct caaggcaggc tcataaaaca
      721 aaattcaaga gcacggtacc cggcattacc tatgctcagt tggtggcgca gtccaccagc
      781 attgatataa aacgcgctaa caacaaagtt tctgatggca cagggatcaa agccgcgaca
      841 ttcctcgggc taaaacacaa ccttgcattg atatctgtta atgcctcgga tgagtcggtc
      901 caccagcatc accgtgtcag aattcgattt gaggaatggg ataaagccgt tgaggatatt
      961 gctgaagacg gtgcgaaaaa agcccgaatc gctgccgatc tctgcaaggg ccgggtatct
     1021 ttcgactgtg attgtggacg ccatcaatac tggtatcgtt atatggccac ggctggtaac
     1081 tatgctgtcg cgccgccaaa agagtatgca ttccccaaaa tccgcaaccc tgatctgact
     1141 ggtgtggctt gcaaacatgt tttgcacgct atgacgcgtt ttcagtctcc cacatggcac
     1201 aaggccatca ttattgccct ggaaaaagca gctgaacagg tagccttcgg cgatgacaag
     1261 cggaagacaa caacctattt caaaggcgaa ctggctaaat cgctcgcgcg caaccggaca
     1321 acaacgacgg atcaggctaa agctgcgcgt gagtatgagc tgtatctgaa atctcaggat
     1381 gcattaggca aaaaactacg cgcaaaagat agcgccacgg acaacgttcg ccggttgtta
     1441 aaaaaagctc gcaccacggc aaacaggaag aatgccgaac taaaagcatc gcgggtgagg
     1501 gaagcccagg ctcgcgctga agccgacgct ctcaaaaaag ccctgcaaac gcaggcgaac
     1561 aacctcataa agtttttcat gagtcaggga atggacaagg ccgctgccac cgcgcaggcg
     1621 cgaagcattc ttgagacaca aattaatgaa gcccgtaaac ggaaaggata atcgatggct
     1681 ggtttctttg atgacatgtt tgaggacaca gaaccatcac aacaagtgac tggtgataac
     1741 ctcccggaca ccgaatcgga tccggatatt ccaggcgaag gttctgaact gattgaagag
     1801 gaagatattg atgctgaaat cgaaaccgat ggtgttaacg ttggtaatat tgttgatcct
     1861 gtggaggaca atcaccttcc caatctggat cacggcctgc ttagtgattc tggtgtgcgc
     1921 caccgttatc aaggtcatgc agtttttaat aaccttgtgc ggatggactg gctcaaagca
     1981 atcaagctag accctgactc attcgatgca gttctgtatc gcgcaatacc ttacagaaac
     2041 aaaaatgcac ctgaaacggt acctgaaata atagaaccga accaacgcat atatgactat
     2101 caggatccag aactgataac ggccctcgac tgcccggatg agatggacgc cttctacgcg
     2161 ctatacgacg gcagtgataa tacgggaatt agcgacagtg ctttaatcct tcggttagcc
     2221 gccgtcaatg tgccagtggg ttctatgctc gaatggctgg aacagctgtc agacggcaca
     2281 accattcgcc gcttctggta catccataaa atattcaatt acggcactgc cagggtaggc
     2341 agtttgtttt attgcgtgcc ttcacgcgcc tttgaaggga atttcatcgg tgattctgaa
     2401 taatcaggaa tggctactgg ccatctttaa gaaaaaaggt cttactccaa ccggtaagct
     2461 ggaatttgcc actattgatg gcattgattc ggcgctcgca caggctttaa acgaagcgtt
     2521 cgactcacaa gttgtcagct ttaatgatcg cattaaccag tcgttccggg agttcctgaa
     2581 acgcacacca agagatcgca taacgctcgg cacttttagt gatgtgaagg agtggttgtc
     2641 gtcatttgaa gccgatcgcg ccgggcgcaa agatacagcc tctgctggcc cggtaaataa
     2701 gctggcaatg ccgcttgtga atctgtctcg ttctcccgcg ttttcaattt atgaaggtga
     2761 actgtgccgg gataattacg atgaagggca tgtcaccaat gaaaatgatg agattgaagc
     2821 cctggtatcg actatccctt tctcactgga atattcgcta tggatcgcca gtgacgagaa
     2881 ggaatctctt gggatggtta caactgcatt agcattctgg ctacgaatgt atgccagcct
     2941 cgggcaggca tctttcactc acattgccaa tgtcggcggt tatgagatac cggttacctg
     3001 ttacatagaa gggcaaaaat caatcgcatt tcaggatctg accaccggca ccgccgataa
     3061 caggctgttc gcggttggat tgaacctcac agtagtggcg gagcttccta tcctggctta
     3121 tatgcagcaa accaccggca ccataacggt aaaagcgaaa attctggagg aatgagatgg
     3181 ccacaaagac caccacagcc ccagaaactg attcaaaacg cactcagcta ttcctgcaat
     3241 ctgtttcaat tgggcagaac gaaatccctc gcgaaatgat cgtaggatgt acctatgtcg
     3301 aacccgggga gctatctggt ccccagctta tgctcatggt cagggattca acggcttacg
     3361 tggtcaataa gctgggggtg aaatttggaa caatactgac tgtttcactt ggtgatccgg
     3421 aaggtcatgg cggcatcctg ttctcggaag agttcttcgt tcttaaagcg ccgcgcaagg
     3481 acgataccgt actgatttac gcgtttagta acccggtgcg gttattgaaa gttccgtcca
     3541 ccagcgcaca gtattttgtt gataagcccc catcagccgt agtttccact cttgcccctg
     3601 gtctgaaggt aaatgctgat tcattcagaa aaacatccac ataccaccta aatgttggag
     3661 aaaaaccgac caaggtattg caggagatag cccgggatac cggttctatg tgctgggcat
     3721 ccagggggac gatcaatttt aaaagtatgg aaaaaatggc aaacgccgct ccatctctta
     3781 cttatgagtc cgccaatccc aacacatccg gatttacaat tagtcagttc aacatcctga
     3841 atgccgatta tgaataccag cgccgccaca attacagaat ggccagttat gacatgacca
     3901 aaggtgtggt ttactcaggt aaccaggaag accccattaa atttacgagc aatcccgatc
     3961 ctaccgcgct ggcgaactac aacaaattca ttctcccccg cctcgatatg ctggtggaag
     4021 gaaatgccgc gctaactccg ggtacgacgc tgaaaattgt cgtgcataac acggctggtg
     4081 acggagaact cgatgaatct atccctgaca aaatgatagt gatgtccgtg actcatttcg
     4141 aagaccgctt tcgttttgtc agccgtgcac agttaggagt ggtgaatggg tagtttgaca
     4201 gggaagtatc gggctgtagt gataagcgtc gatgacccta aaggtctgat gcgtacacaa
     4261 atacgtgttg tcggcatgat ggatgggtta ccagatgcct cattgccgtg ggcagaagct
     4321 atattgtcca atgcaaacac gttttcacca tttctgcccg gcgataaagt atgggtagaa
     4381 tttccctaca atggggattc gcgatggcca ttgataatcg gttatgcaca ggatgcatcc
     4441 ggtggcgctc ccaatgtgcc gcctgaagcg tcaggacaag gtgaaggcta tgtaccgcct
     4501 gaagttgaag gtgcaccagc acaaccatca accagcgcca aaaaagactt tatttcttcg
     4561 cggaacggac taatggagat ccggacggcg ggcggagcct gggccgttac gcacttgaaa
     4621 agtggaacaa caatcgggtt caacgaggcc ggggagttat atgccatttc tcaaggtccg
     4681 gcattcatct cttccgcagg aaatctcgat ataaagtcag gcgcggatgt cgccctgaag
     4741 gcggggggaa gtatggcgat agaggccagc gggaacctat ccataaaagc cgctcaagtc
     4801 tctgttgaca aggcttaaga aaagcccggc gttcgggctt ttctgttatg acgggttcaa
     4861 ttttttatcc gttaccgcgc gacggtttct gcgtgataaa cgtctcaagc atcttttccg
     4921 caattgccga ccaggtgtga cactggacct tttcagcatt tttcacgcga tcaacgcgag
     4981 caataacctc atcccaatca atccgcgact tgataaccat atggttcacc aaagccaagc
     5041 gatctggcgg aaggcaatcg ggaggcgtta ataccaacgc cccgcacatt gccgcctcaa
     5101 gtacagttaa tccaaggctt tcgggatgcg taacgataaa cacgtcactc ttacgcaatt
     5161 cagctgcaaa ttcggttgct ggtaccggcg tccgtctgta tggggttacc gatatattcc
     5221 ccggatcaat ggtaatcaat ccgtcatcgg tcaacgttct ggcctcatac ggaacggtca
     5281 gacgctgaag gttcataagg atacttaagg agtgatcaaa cccactaaca tcaaatgcag
     5341 cgtggtctac aaaaatacgc agaacatcgt ctgttttggt ttccagatgg aacagatcct
     5401 gattcgctgc ccatccaaca tgtttgttaa agcgattatg acgttctaac cgaccgggat
     5461 tatccaggta ccgccaggta tcatcgcgga cagtaaaagt aatatcgact ggtgccgaat
     5521 ccagcataga accgtcatat acctgggcta cccatccaga gaatcggcga cacagttgca
     5581 tgcctatttc cctgggtacc gtagtaaaat acctcaatcc tggtgccaaa atggccttcg
     5641 cagaacatgc tgtcgcagca gtcaacacag cttcaacata atcctcgggg ctttcgacgc
     5701 caggggaata tggacgatgg tattgcaatg ttacccctgc ctcactaaag gcgcaggcca
     5761 ggttataaga ccacatttcc gtatatgttt tcacatcact gatggctgca aattttcgcc
     5821 caatgatcag gatgttcatc ggcttttcct cattccattg cattaataat cctcttgcca
     5881 gtcagcacca gcatagttat caaaccgtga gtattggccg ttaaaagcca atctcaccgt
     5941 gccaattggg ccatttcgtt gctttccgat aattacctcg gcaatgccct tcatttcgct
     6001 atccgggtga taaacttcgt cgcgatacag aaacatgatc aggtctgcgt cctgctcaat
     6061 tgctcctgat tcacgtaaat ctgaatttac cggtcgtttg tccgcacgct gttcaagcga
     6121 tcgattaagt tgtgacaatg ccaccaccgg tacttgtaat tccttcgcca aagccttcag
     6181 tgagcgagaa atctcggcaa tttccagcgt tcggttatct tgcagctcgg ggacgcgcat
     6241 aagttgcagg tagtcgatca taatcatgct caaaccacca ttttctttat aaacacgacg
     6301 agcgcgggaa cgaagctctg taggtgtcag ggcgcttgag tcatcaataa aaatattctg
     6361 cttgtccaac agaatcccca ttgcgccaga aacccgcgcc caatcctcgt cgttaagttg
     6421 ccctgttcga atacgagtct gatcaacgcg tgcaagagaa gccagtgagc gcatcatcag
     6481 ttggtggctc ggcatctcaa ggctaaaaac caatacgggc ttatcgttac ggactgcggc
     6541 attttcgacg agattcatcg caaacgtagt cttccccatc gatgggcggg cggcgacaat
     6601 aatgagatcg gacgcctgaa gtcctgccgt cttcttattg agatcggtaa atccggtatc
     6661 aagccccgtt acaccatcat gcggtcgctg aaacaactct tctatgcgag ataccgttgc
     6721 atcgagaatg ctggcgatat cttttggacc actaccgctc ttttgtcgtt tttcagctat
     6781 ttcaaaaacg cggcgctcgg ccatatccag caattcattg ctgcccctgc catcctgcgc
     6841 atatccagct tcagctattt catttgcgac ggaaatcatt tcacgaacaa ccgcgcgttc
     6901 acgaacgata tccgcataag cacaaatatt tgccgcgctg ggcgtgttct ttgacatctc
     6961 cgcaaggtac gcaaaaccac cggcgcgttc taatttaccg ttctgttcaa gtgcttcagc
     7021 aagtgttatc aaatcaatcg gtttgccatg acttaataac ctctccatct cactgaaaat
     7081 ttcacgatga gcactggtat aaaaatcatc agcaactata cgatctgcaa cttcatccca
     7141 gcggcagtta tcaagcatta agccaccaag tacagcttgt tctgcactaa gggaatttgg
     7201 catggattca agaggggatg cagacattag cactccaccc aggcgtgctg aatgtcagat
     7261 ataatcggca tactcaaatc actcctaacg atatgagtca tcaccagaaa atcaggatta
     7321 atgcgccgga ctcttcccgg ctgtcacacc gaatcgccag gatggtgaat ccgcagtccg
     7381 acgctatgaa cggggcttgc acattccggc tacctggttt gttgcctgag ctaggggaaa
     7441 ggttacccct ttaacgtcac cagaccgcta acgacgcatg tgccagacgc cgtgttacaa
     7501 ccaaatatgg tggcccctac cggacttgaa ccggtgaccg tgcgattatg agtcgccagc
     7561 tctaaccact gagctaaagg gccggattac tgccaatttt gcttacgctt ttatttcacc
     7621 ggaacaaacg gaacagcggt attactggtc atatactgcg gtaatgtacc gttccatttg
     7681 ttgatcgctt ccaagtccat aacaccgggg ttctggcgca gagcttcacc acgtaaacga
     7741 atggcatcag cttcggcctg ggcttttgtg cgaatagcat cagcctgtcc ggcagcttcc
     7801 gcgcgcagca tgttggcctc tgcttcacgt tgtttgacct cttgctcgcg ttgcagggtt
     7861 ttttggtttg ccgtgacttt ggcgttaata ctgtcaataa ctgttggcgg gtattctggc
     7921 ttacctacat aagagaggct catcacctga atgccgattg gcgtcatttc ttcctgaatg
     7981 tctttaaggg ctgcatcaag caattcagat ttgccaccgt cgataaattt gtcggtggtc
     8041 attttgctgg ctaatcggtt cagagcatct gcaaccttct ggcgtagatc ggtatcagta
     8101 atatcatcta cacctttgcg ataggtctga aataccgttg tgacttttgc tggatcaacc
     8161 ttgtaggcta cgccgatgtg gtaaccaatg gttgttccgt cgctcatctg gaagctgaac
     8221 ggctcatcgt atgtcttcat ttgcttaaag gtcgggaaga tataaacttc agtattcaag
     8281 cctgtccagt agcgaccaac gccaactact tcaccgatac ctttatcatc ccccagctta
     8341 tttactttga tccctacgtt acctggctct acccgatcgc atccggtcag acataaagaa
     8401 cccaaaataa tcgctgcact aatcaacgtt tttttcatta attaatttcc tggttttttc
     8461 acgaaaaaag actactgcga aagccgggta aatgagcgcg agaaggactc ccaacaatac
     8521 aagtattgtg ctgttagatg agatcatatt tggcaaaagc caaacataca gaaccagtga
     8581 cacaatcaaa cagaggacgg cataaatata taaccgcacc catagcgttc gacatttgtt
     8641 cggattgttc tgcatcctct cactccatta tttaacgaat aaaaaagctg cggtgccggg
     8701 ttcctcccgg tgtcctttgg ctggttatcc accgtggacg tggaaacaag gagaaataat
     8761 ggacagatat aaccatttcc ccgcgtgcgc ttagccgcat tcaccgcaac ggaaagagca
     8821 ttcttggtgg acctgtagat tgggatatga acccgttaca ggagaatgct cttacctgtt
     8881 acgtgctccg tttcgtggag ctaacggcgg gtgatcagac cgcaccagac tggacttatt
     8941 tcagcgttat gctcatgcca gagaatcaaa ctgtgatggt cggtgctgaa ctccgacaca
     9001 gggttgtagc aagccccgca aagcgcgcac tactgtagtt gcggcacatc agcctgtgca
     9061 ttcaccacaa tgttgagaac actggttgtc acgcggcaac gcaacattta ttcgtagatt
     9121 gggatatgac cccgttacgc cagtgttctc aacgttgtag ttgttatgcg tattctctta
     9181 ctaaccgcac accaatacat ttcccatcac gttcatttgt ctcgaaacgt gaatacttaa
     9241 gttcgccgtt aataatcgct gttacaaccc gtatcccgtt aaacgcccac atatttcgct
     9301 ccatttacac acattaccag tcgccggtta cggttccggc caggcctctt cctcaacggg
     9361 gtgttctcca tacggactac cgtttattgg tcgttcctgc ggtttatgtt gtgaagccag
     9421 atgcttatct tctggttgct tctaagatct gcacttcatc acaacggtaa gagcactcga
     9481 tgcatttaag ccaggcccca taagggagaa tgctcttacc tgttatgttg gtgccgatta
     9541 acgggatcga accgctgacc attcgcttac aaagcgactg ctctaccaac tgagctaaat
     9601 cggcaatgtg gtggggagtg atggagtcga accacccgag tcgcaatgac agtagattta
     9661 cagtctaccc cgctacccct acggactaac tcccctaaag tggcgatggt gggtggattc
     9721 gaactaccga ccagttggtt aacagccaac tgctctaccg ctgagctaca ccatcacttg
     9781 ccgggtacgt ctccggcgag ggcttccacc tccgtatgct tttcggcgca ccgcgccctg
     9841 gctgcaattc ggtaacaggg gatgcataac cctggcttcc agcgtgatta gcgccttcag
     9901 catgacggga tatacccgta aattcgtgga actgtaccca aagtgctgtt aagcaccgct
     9961 gttacgctga aaagaaaacg caacaggaaa ggacgctgac caacagatgg ccccttctcg
    10021 ttcatctggt taatcacacc agcgccctta cctgttgtgc ctccccgttc cctaatacac
    10081 agacggggac actctgcggt cgattttttg acgggggacg actcataccc catggcatct
    10141 ggcttcttag gccgctacca tcatcagatc atcgtttgca tttactttaa tggtcagttt
    10201 ctaaaccgcc gcaaagtcgc taaccatgac gaaaaccctg aaaaaaacgc ccacccgaag
    10261 atgggcaaac tggacgctcg taacgcactt cggcgttgcc acttaggcgc atggtcaacc
    10321 tggcaactcg gtggtttgtc tgggaggact aggcccagcc atgcttaccg ccgcgcctgt
    10381 cgcggctaac agctaaatcg ctctataaat cacgattcat tgaggtgata ttacactaat
    10441 aaatttatta gagcaatata cccaaaacgt catgagctac acctcgagtg tcccccttac
    10501 aagacacaga acgtctggca aaaagaggtt ccactctgaa gccactgtca tgataaagct
    10561 ctctgatgtt tggcgcgcca ctgttagtaa tgagaacctt tgcacctcga cgatgagcat
    10621 ccgtcaacag agacaccagg cgtttttgct cttcaaactt aaagtcatga ccggaatagt
    10681 tcgtgaatcc ctctgtattt ggaagcggtt catacggcgg atcgcaaaag atgacatctc
    10741 cttctccggc agcttcaatc accgctgcaa aatcaccgca tacaaactca gaccgccctt
    10801 ccgcaccgag gaaggcttcc atctcctgta atgggaaata cggagtttta tacttcccat
    10861 aaccgacatt gaactcaccg gcctggttgt aacgcgtcaa tccgttaaaa caatgtcggt
    10921 tcaggaacaa aaacgccgct gcgcgatgta aatcatcata gacttgtttg ttaaacgcat
    10981 tccgtactgc caggtatcct tcctgtgtgt tgtagtcctg gaagaaacga tgtgccagag
    11041 tgataagtga atgcgcctcg cgttgcagag tcttgtaaaa gttaatcagg tcagcattca
    11101 catcatttag cagattttcc tggtatccgg cattcatgaa gacagctccg ccaccaacga
    11161 aaggttcaat caggcgcttc ccttctggca aatagcgaaa gatttgttcc agaacaccaa
    11221 attttccacc agcccatttg aatatggacc gttcgaattc tgccgctggt ttaacttttc
    11281 gctcttttgt ttcacttcct tctttctgcc gacatacggc cttagtaatc cgatcgccaa
    11341 tccagcgcat tactggtatt gccatactat tgccgatcgc tttgtaacgc ggtccgtcag
    11401 ctgcaagcat cgcggcctct tcttcgctta aatctggata gtgattgcga aggtatgcca
    11461 gttcatctga agaaactttt ttacgctttt ccgtcgggat caacgtatgc ccctcaggaa
    11521 aaccttgcag cctttcacat tcgacagggg taagacggcg gacagctact tctgcgtttc
    11581 ttacttcata gcaaacagct gttggatttt tagccattag agatggtgaa gtattcttag
    11641 ttgcagcatg ttgtgtaccg ctcatacgct caggaaaagc caatgtaaca agatgctcat
    11701 ggctttcttg ctcacgtgcc cgcaatgtac catgcccttc tgaccaaaaa cctgctcctg
    11761 tgctgctaaa aacggcaagg tcagtggcat ctttaaaatc tcttgccttt actgtcgatg
    11821 cggtttcatc gtcaatatat tccccaaatg ctgccatcct gaaagcgttt acggctttcg
    11881 tcgatttcat accgggtggc atgtcagcgt gtaggcatgg atttaggctt tcgccactga
    11941 ttgcagcgcc atttgcaata atggcggaag cgatttcctt cttttttcgg ctcggcgcaa
    12001 tattccggcg cacgccttcg aactcaaaaa gtaccgttgc gggatcgaga tctgttcgag
    12061 cacttgcgac aacaaacacg cgtcggcgtc gttgtgccac tccgaagtat tgggcatcaa
    12121 ggattctcca ggccactttt cgctgcggtc cataaataca accacactgc ggccactttg
    12181 gaacatggca accggttttg ccatcccacc gccagaacgc gttgcttttt cctgattcag
    12241 gtcgatcacc tggttcaaat ggcacatctt ctccagccaa tccggcaagg aaacatccga
    12301 aggcgttatc tgccgatgac aagactcctg ggacattttc ccagacgata acggctggtt
    12361 tgagaaatga ctcagcccgt ttgtcgtcaa ttgcatttgc aagctccaca tactttaaag
    12421 ttagcgcgcc acgctcatca tcaagcccac cacgtaatcc cgcgatactg aatgcctgac
    12481 agttatggac caccgcacca ttgaggatat aggaatgatc accttcgact tctatgttgt
    12541 atacagtatc taggcctacc gatttaaact ctttgactgt tcgtaaaagc attccatgag
    12601 ccaatcttga tttacgtgac aatttctgcg ggcagattgt tacctgataa taattccgtt
    12661 gatttaccac gcgatcctcg atcacttttt tgggctcaac ttcaataaag ctgaccgaag
    12721 aaacataacc acaagtctgt gacaaccccg caacgcccca agcaagcgca gaactaacac
    12781 tattaattct aaatcccgct ttaccacttg gtgtcccatc agtatcaaga tagccttgta
    12841 aaaacacatg acgcaatgga tgcgacatca cccatgcagg gatacgctta gcatggctta
    12901 actcgccaaa atgttcatta agccaattgg cataacacgt atcattcaag gttactttga
    12961 tgctccctcg gatttcgctt gccacggaaa atatgttttc tggtatgcgg caatgaaact
    13021 ttctcaattt ctggcaattt atgccaaaaa caaccgcctt cttagattta cctctccatc
    13081 tcctaatata tccatcgcca acataagcgc ccgcaagata catagcctgt tcttcagaca
    13141 agaaccgaga acaaatatct ggaaatgcaa tattgaaatt agttagagcg caccattgat
    13201 atcccggcat atcacatgct gctcgccatt ccggttcaga caacaactct ctcttaaaat
    13261 atgtgccatt tttccgggtg ttttgggctt tccaccgaac agccaggaag ggatggtcat
    13321 tggttgttct tatacctaaa ggctgcccaa cggcattaag taaccccgta ttagctattt
    13381 ttgaaccaac tctttttact tgttgtaacc gcccgagatg actgactacg taatcaccag
    13441 ggcaaacatt ttctattggt ttatagccat ttttacaaag aaccatatgc cccgcggtaa
    13501 aacatggtgt tcccccgacg agcacgtcag gggattcgat ttccccagcc aagacttttt
    13561 tggcaagttt tttcatgtcg ccaaggttgg caacatgggg ccagcggagc gcaagaacgg
    13621 ccgatggaaa aggctcgatt tcagcaaacc acgccggacg catacccaac ggttcccagg
    13681 caatactcgc ggcttcaatt ccactgcaaa cagatccata gcacagctct ttcactgctt
    13741 agcctctccg ccaagggcat ttaccagagc atcaaccagg cacgaaattt cactggtcaa
    13801 caggaagaaa tctgcgtcca gtcgctgcgc aacatcttca ctatcaatat cagagttctg
    13861 ctcaagcaat tcatccgcaa atttgacgct ggtaaggctg aagttatggt ccagtgtaaa
    13921 tttaatgcgg ttctgccagt cgagtgccaa cttagtgacg agcttgccag cttccaggtg
    13981 tgtggaaatt tcatcgcttc ccaaatcctg ctttttcact cgggcaatac cgccatcctc
    14041 aagcactgcc ttaagttctg ccgcatcccc catttgaaat ccctgtggag cactaccatc
    14101 acgtacccag tcggtcagcg ttaattcaat gggattttca acactcaggg gaacagcagg
    14161 aagagaaccc agagacttac gcataagcgc gagcatatcc tctgcctgcc gcgcgctggc
    14221 attgatatag atacgtttag ttgaaccgtc gtagatcgcc tggataacag aaaactttga
    14281 aaaagcccgt ggcagaagag aatgcagaac ttcgtctttc agggagtcct tctctgtttt
    14341 cttcagttta cgcgcttgtt cttgctcaag tttttcaatt ttttcttgaa tagctcgctg
    14401 gataaccggc gggggaagaa tttttgtttc gcgctttgct tcaacaagga taaaaccatt
    14461 tccatgcata gcgataactt cagaattatc accaaatggc gatacaaaac cgaacttggc
    14521 catatcctga ctaccgcatg gcgtgaaaag gatcattttc tttttatctt ctaagtcggt
    14581 cagatccgcc tcacgagaaa gtttataaat agtaatgttt ttccagtgct taaacatgtt
    14641 gtaacccttg aatatcaacc acagaaagct cgtctttgta gaaaaaggcc aggttgtggc
    14701 accccctcgt ttgagcgtat gagctgggac caatttcgtt cttccagaca aatggcttca
    14761 aatccgtacg gcgaagcata aaaacgcgat ttattccgct ctgattccca atgaggcaaa
    14821 agccttcttt caccttgata gcctgcaagt tgtcgagttc accgctggtt acacggctat
    14881 cgaactcttt gcggcttatt agctccatct gcatctgacg actccaaaca aatgcccatt
    14941 gaagggcgat ggctgaatgg tac
//
LOCUS       DAZUFB010000117        14628 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZUFB010000117 DAZUFB010000000
VERSION     DAZUFB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.117"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..191)
                     /locus_tag="ACHY3M_004058"
     CDS             complement(3..191)
                     /locus_tag="ACHY3M_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFS4318874.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(195..554)
                     /gene="yeaR"
                     /locus_tag="ACHY3M_004059"
     CDS             complement(195..554)
                     /gene="yeaR"
                     /locus_tag="ACHY3M_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFS4318875.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(727..1365)
                     /gene="leuE"
                     /locus_tag="ACHY3M_004060"
     CDS             complement(727..1365)
                     /gene="leuE"
                     /locus_tag="ACHY3M_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFS4318876.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1492..2415)
                     /gene="dmlR"
                     /locus_tag="ACHY3M_004061"
     CDS             complement(1492..2415)
                     /gene="dmlR"
                     /locus_tag="ACHY3M_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFS4318877.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2518..3603
                     /gene="dmlA"
                     /locus_tag="ACHY3M_004062"
     CDS             2518..3603
                     /gene="dmlA"
                     /locus_tag="ACHY3M_004062"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFS4318878.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3854..5464
                     /gene="yeaV"
                     /locus_tag="ACHY3M_004063"
     CDS             3854..5464
                     /gene="yeaV"
                     /locus_tag="ACHY3M_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFS4318879.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5496..6620
                     /gene="yeaW"
                     /locus_tag="ACHY3M_004064"
     CDS             5496..6620
                     /gene="yeaW"
                     /locus_tag="ACHY3M_004064"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFS4318880.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6676..7641
                     /gene="yeaX"
                     /locus_tag="ACHY3M_004065"
     CDS             6676..7641
                     /gene="yeaX"
                     /locus_tag="ACHY3M_004065"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFS4318881.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMGDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7695..8810)
                     /gene="rnd"
                     /locus_tag="ACHY3M_004066"
     CDS             complement(7695..8810)
                     /gene="rnd"
                     /locus_tag="ACHY3M_004066"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFS4318882.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8892..10577)
                     /gene="fadD"
                     /locus_tag="ACHY3M_004067"
     CDS             complement(8892..10577)
                     /gene="fadD"
                     /locus_tag="ACHY3M_004067"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFS4318883.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEYQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10782..11363)
                     /gene="yeaY"
                     /locus_tag="ACHY3M_004068"
     CDS             complement(10782..11363)
                     /gene="yeaY"
                     /locus_tag="ACHY3M_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFS4318884.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11403..12098)
                     /gene="tsaB"
                     /locus_tag="ACHY3M_004069"
     CDS             complement(11403..12098)
                     /gene="tsaB"
                     /locus_tag="ACHY3M_004069"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4318885.1"
                     /translation="MRILAIDTATEACSAALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12156..14066)
                     /gene="yoaA"
                     /locus_tag="ACHY3M_004070"
     CDS             complement(12156..14066)
                     /gene="yoaA"
                     /locus_tag="ACHY3M_004070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4318886.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14198..14542
                     /gene="yoaB"
                     /locus_tag="ACHY3M_004071"
     CDS             14198..14542
                     /gene="yoaB"
                     /locus_tag="ACHY3M_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS4318887.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3547 a         3881 c         3622 g         3578 t
ORIGIN      
        1 ccttatatcc ccgccctgaa ggacggggtt ttacggcgca ccggataaga acgcacggta
       61 ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc
      121 tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac ggtatacgtt
      181 gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc accttccatc
      241 aggacttcag gagccacgaa gaagtcaatg ttgaaataag tatcgtcagt catggcttca
      301 atgttgtgcc acttttctgg agggaacacc gcaaactgcc ccgcttcgat aaggatcacc
      361 tgatcaggct ctgcactgtg ttcatcagcg tagccgagat atttgaccgc cccatgcata
      421 acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt
      481 ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt
      541 gggatttgca gcatcctatt ccctccttcg gggaatgcgc tgaaaacggt ttattccagc
      601 cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt actttttagc
      661 accatatcag agaaaaatca gggaggatta caagtgggga gcccgcgaca acgcgggctg
      721 aatgtatcag gattgcagcg tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat
      781 cagtgagttg ccaactttag ccagtttctt tttggtacgt atgtactgcg tgacaaaagc
      841 accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag
      901 aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga
      961 cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat
     1021 ggccccgtat tggggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta
     1081 gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta caatgttgaa
     1141 taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac
     1201 tgcatcgcca ataaatacac cgcaggccgc aagataaccg cctttcatac cgctactgac
     1261 gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc
     1321 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
     1381 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa
     1441 aggagaaaat caggttttaa cccgatatca acccgataat tgaatcatta atcaggcatg
     1501 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat
     1561 ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt tcgctggctg
     1621 gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc taacatccca
     1681 ccaggagcgc agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg
     1741 attagacgac agtgggccgg tgactttaat cgcatggtgg ccttctttat tgcgtaattg
     1801 ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cggaaagatc
     1861 ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggcgacgcgc agagaatgcg
     1921 gtaattagtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc caatgcgaat
     1981 atcgagatca acaccttcat tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa
     2041 ttccagttgc ggatacgcct tagccagcgc tgataacgcc ggagccacca cctgtcgtcc
     2101 aaaaccaaag ctgctgataa tacgcagcat cccctgcggc acctgacgca cgtcagaaag
     2161 ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat aaatccgctc
     2221 tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt
     2281 ttgctccagc aaggcgatgc gcttgctgac gaacgccggt gaaacgccca gttcttccgc
     2341 cacggcggca aaaccagccc gacgagccac cagcataaag acgcgcaaat cattcagcag
     2401 cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacggggatt aattcctttt
     2461 cagtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga gaacccaatg
     2521 atgaaaacga tgcgtattgc tgcgatcccg ggagacggga ttggcaaaga agtccttcct
     2581 gaagggattc gcgtgttaca ggctgccgct gagcgctggg gcttcgcctt gagttttgag
     2641 caaatggagt gggcgagctg cgagtattac agccatcacg gtaaaatgat gccggacgac
     2701 tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg ctggccggac
     2761 accgttcctg accatatttc gttgtggggt tcgctgctga aatttcgtcg tgaattcgac
     2821 cagtacgtca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc gctggcggga
     2881 aaacagcctg gcgacatcga tttttacgtg gtcagggaaa acaccgaagg cgaatattcc
     2941 tcgctcggcg gtagagtgaa tgaaggtaca gagcatgaag tcgtcattca ggaatcggta
     3001 tttacgcgtc gtggtgtcga tcgcattttg cgttatgcct tcgaacttgc gcaaagccgc
     3061 ccacgtaaga cgctaacttc tgccactaaa tcaaacggtt tagccatcag catgccgtac
     3121 tgggatgagc gagtggaagc aatggccgag aattacccgg agatccgctg ggacaagcag
     3181 catattgata ttctctgcgc gcgttttgtg atgcagccgg aacgcttcga tgtggtggtg
     3241 gcgtccaatt tatttggcga tatcctttcc gatcttggcc cggcctgcac cggcaccatt
     3301 ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct cttcgagcca
     3361 gtccacggtt ccgcgccgga tatctacggg aaaaacattg ctaaccctat cgccacgatt
     3421 tgggccggtg caatgatgct cgattttctc ggcaatggcg atgagcgttt ccagcaagcg
     3481 cataacggta ttctggcagc aattgaagaa gtgattgctc acgggccgaa aacacctgat
     3541 atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat tattttgcgt
     3601 taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gtttttttat tcgttctttg
     3661 cagtaaataa cctgcgtcat ttcacctttt attgtttccg tttcgtgttt tatggctttc
     3721 cgtattctta attgtttaat ttatgtaaca tgcaaataat caatttgtta aacacaacat
     3781 acaactcatt accctgcaaa taattacaac tggcaaaagc cagagacggg cttttatgcc
     3841 caggagatga ctcatgatga gcaatgttaa gaaaaaagat gtgccgctga taagcatcag
     3901 cctggtggcc attcttttca tcgcagctgc attaagcctt ttcccacaac aatcggccga
     3961 cgcggccaac gcaatataca cttttgttac gcgtacgtta ggttccgccg tacaggtatt
     4021 ggttttgctg gcaatgggac tggtgattta tttagccacc agtaaatacg gcaatattcg
     4081 tcttggcgaa ggaaaaccgg aatacagcac gctctcctgg ctgtttatgt ttatttgtgc
     4141 cggtttaggt tcttctacgc tttattgggg ggttgctgaa tgggcctatt attatcaaac
     4201 gcctggatta aatatcgcac cgcgttcaca acaggcactc gaatttagcg tcccttactc
     4261 cttcttccac tggggcatca gcgcctgggc aacttatacg ctggcctcat taatcatggc
     4321 ttatcacttt catgtgcgta aaaacaaagg tctgagtctt tccggcatta ttgccgcgat
     4381 taccggcgtt cgcccgcaag gcccatgggg aaaactggtt gatttgatgt tcctgatcgc
     4441 cactgtcggc gcactgacta tttcccttgt tgtcaccgca gcaaccttta ctcgtggact
     4501 ttccgcgctg accggtttac ccgataactt caccgtgcag gcatttgtga tcctgctttc
     4561 cggcggcatt ttttgcctaa gctcgtggat tggtatcaac aacggtttgc aacgtctgag
     4621 caaaatggtt ggctggggcg cgttcctgct gccattactg gtgctgattg tcggcccaac
     4681 cgaatttatt accaacagca tcatcaatgc catcggtctg accacgcaaa acttcctgca
     4741 aatgagctta ttcaccgatc cgcttggcga tggttcattt acccgcaact ggaccgtttt
     4801 ctactggctg tggtggatct catacacccc tggcgtagca atgtttgtca cccgcgtttc
     4861 ccgcggtcgt aagattaaag aagttatctg gggactgatc ctcggcagca ccgtcggttg
     4921 ctggttcttc ttcggcgtaa tggaaagcta tgccattcat cagtttatca atggcgtaat
     4981 caacgtccca caggtgctgg aaacactggg cggcgaaaca gctgtacagc aagttctgat
     5041 gtcgttgcca gccggtaaat tgttcctcgc cgcatacctg ggcgtgatga ttattttcct
     5101 tgcctcgcat atggatgcgg tggcctacac catggctgcg accagtacgc gtaatctcca
     5161 ggaaggtgac gatcctgacc gtgggctgcg tcttttctgg tgcgtggtga tcactctgat
     5221 cccgctttcc atcttgttta ccggtgcttc gctggaaacg atgaaaacca ccgtcgtgct
     5281 cacagccctt cccttcctcg tcattttact ggtgaaagtc ggcggattta ttcgctggct
     5341 gaaacaggat tacgccgaca ttccggctca tcaagttgaa cattatctcc cgcagacacc
     5401 ggttgaagcc ctggaaaaaa cgccagtgct ccctgcggga accgtattca aaggcgacaa
     5461 ctgagcgcca tcatcctaac gataaaggta tccctatgag caatctgagc cctgactttg
     5521 tactacccga aaatttttgc gctaacccgc aagaggcgtg gaccattcct gcccgttttt
     5581 ataccgatca gaacgcgttt gaacacgaaa aagagaacgt cttcgccaaa agctggattt
     5641 gcgtcgctca cagcagcgaa ctggcgaatg ccaatgatta tgtgacgcgt gagatcattg
     5701 gcgaaagcat cgtgctggta cgcggtcgtg ataaggtttt gcgcgcgttc tataacgtgt
     5761 gtccgcaccg tggtcatcag ttgttgagcg gtgaaggaaa agcaaaaaat gtgattacct
     5821 gcccgtatca cgcatgggca ttcaaactcg atggcaatct ggcccatgca cgtaactgcg
     5881 aaaacgtcgc caatttcgat agcgacaaag cgcaactggt tccggtgcgt ctggaagaat
     5941 atgccggatt cgtcttcatc aacatggacc ccaacgccac cagcgtagaa gatcagttac
     6001 ccggtctggg cgcgaaagtg ctggaagcct gcccggaagt ccacgatctg aaactggcgg
     6061 cccgctttac cacccgcacg cctgccaact ggaagaacat tgtcgataac tatctcgagt
     6121 gctatcactg tggtccggcg catccaggtt tctccgactc agtacaggtt gatcgttact
     6181 ggcacaccat gcacggtaac tggacgctgc aatacggttt cgccaaaccg tccgaacagt
     6241 cgtttaaatt tgaagagggt acggatgcgg cattccacgg tttctggctg tggccgtgca
     6301 cgatgctgaa cgtcaccccg atcaaaggga tgatgacggt catttatgaa ttcccggtgg
     6361 attctgaaac taccctgcaa aactacgata tttacttcac caatgaagag ttaaccgacg
     6421 agcaaaaatc gctgattgag tggtatcgcg atgtgttccg tccggaagat ttacgtctgg
     6481 ttgaaagcgt acagaaaggg ctgaaatcgc gtggctatcg tggtcagggg cgcatcatgg
     6541 ccgacagtag cggtagtggc atttccgaac atggtatcgc ccatttccat aatctgctgg
     6601 cgcaggtgtt taaggactaa tgacatcggc ggcggtattt tccgccgctg ggctgatttt
     6661 tgatggagta cagcaatgtc agactatcaa atgtttgaag tacaggtgag tcaggtggaa
     6721 ccgcttaccg aacaggtgaa acgcttcacg ctggtggcaa ccgatggcaa accgctgcct
     6781 gcgttcaccg gaggaagtca catcattgtg cagatgggcg atggtgataa ccagtacagc
     6841 aatgcgtatt cactactgag ttcgccgcat gacacctctt gttatcagat tgccgttcgg
     6901 ctggaggaaa actcgcgcgg cggttcccgc tttttgcatc agcaggtaaa agtgggcgat
     6961 cggttaacga tttcaacgcc taataacctg tttgcgctaa ttccctcagc cagaaagcat
     7021 ctgtttatcg cgggcggtat tggtatcacc cctttcctgt cgcacatggc agagctgcaa
     7081 cacagcgacg tcgactggca gctacattac tgctcgcgaa atccagaaag ttgcgcattt
     7141 cgtgatgagc tagtccagca tccgcaggct gagaaagttc acttgcatca ctcatcaacc
     7201 ggaacacgac tggaattagc gcgattattg gcggatatcg aacctggcac acacgtttat
     7261 acctgtggtc ccgaggcgct aaatgaagcg gtaagaagtg aagctgcgcg tctggacatc
     7321 gtcgccgata cgctgcactt tgagcaattt gctatcgaag acaaaaccgg cgatgccttt
     7381 accctggtgc ttgcccgttc cggaaaagag tttgtggtgc cggaagagat gactattttg
     7441 caggttattg aaaataataa agccgcgaaa gtggaatgtt tatgtcgtga aggggtatgc
     7501 ggaacctgcg aaacagcaat actggaaggt gaagctgacc atcgggatca gtattttagc
     7561 gatgaagagc gtgccagcca gcaaagtatg ttgatctgtt gttcgcgtgc gaagggtaaa
     7621 cgcctggtgt tggatttgta gtttgccaaa gccggatgtg gcgctgagcg cgcccagtcc
     7681 ggcttcggaa gattttactg cggatattcc tgcaataaat tgtgtaatgc ttccgccatc
     7741 agctcaccac gccagccgga aatcagctcc ggcaaattgt tctgcggttt cagtttccag
     7801 tgccagttca gcagttggtt gatttgccga cgcgatgcca gcaattcggc gctgatctta
     7861 tgcgtttcgc tcacgtcagt aatcagcgac ttaatcgctt taaacacttt acgataacca
     7921 ggcatgtcca tcaggttaag catcggctgc ggtaaggcct cttccggcaa tgtctgcgct
     7981 ttttccacca gcgccagcag cgttttaccg tgaaagcgga tttcgctacc ggataaaccc
     8041 aggctgtcca gttcgcctaa acttcccggc atataacgcg cgaccgacca caaatgctct
     8101 tcacgcacga caaagttcac cgccagatca cgctctcgcg ccttgcgcag tcgccagtcg
     8161 gctaacagtt gcagacaggc cagttggcgt gtgcgtaact gccaggcatt ggtgatatca
     8221 cgccaggcat cttccggcgc aacgacttcc tgacgacgca tttgcatcag gcggcattca
     8281 tccagcgccg caggtagcca gccggaggcc tccgtttcta ccataagctt ggcggtgatc
     8341 ggtaacagat accagacatc cgccgctgcg tattcacact gacgttcggt cagcggtctg
     8401 gccagccagt cggtgcgcga ttcactcttg tccagcgtaa cgccggaata ctcttccacc
     8461 atggaagcga aaccccatga catcgggcgt ccgcagaagg cagcaaggat ttgcgtgtca
     8521 atcaagggtt gtggtaattc gccaaagaca ttgaggaaca cttccagatc ttcactgcct
     8581 gcatggagaa attttgtgat ggacggatcg cgcaggatcg ctttcagcgg tgaccagtcg
     8641 gtgatcccga gaggatcaat tagcgccaga tgctcgccat cgaaaagttg aatcaacccc
     8701 agctgcgggt aataagtgcg cgtacgaaca aattcagtat ccagggctat cgccggaaag
     8761 gcacggacgg cttcacacaa agaagccagc gcatcgtccg tggtaatcat ttggtaattc
     8821 aaattgtttt ctctttagtg ggcgtcaaaa aaaacgccgg attaaccggc gtctgacgac
     8881 tgacttaaca ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg taattctcgt
     8941 cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac cagcttcggt
     9001 actttgtagc ccgtgagctg acggcggcaa aaggtcacca gtgactcttc ggtaagcgat
     9061 ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga aggtacgcca
     9121 acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat ctcgttggga
     9181 tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat gcgcaggaac
     9241 ccttcttcat ccatcaccgc gatgtcgccg gtgtgtaacc agccattttt gatgatctca
     9301 tctgtagcat ccggacgctg ccagtaaccc agcatcacct gcggtccttt gacacaaagc
     9361 tcacccggtt gacccggtgg tacttcatta tcatcatcat ccaccagttt ggcttccgtc
     9421 gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg gttaacgctg
     9481 accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc tgtcagtttc
     9541 acccaacgct ctgccaccac ttgctgcact ggcatccctc cgcctgcgga aagatgcaga
     9601 ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa caaggtgtta
     9661 acgcccgtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc tggaatatcg
     9721 cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag gcagttaatg
     9781 gtcagggcaa aaatgtgata cagcggcagc gccgtcacca ccagctcttt gcccggatgc
     9841 agcagcggac cataggtcgc gttaacctgt tccaggttcg ccagcatatt gcggtgagtc
     9901 agcatcgcgc ctttcgccac accagtggtg ccgccggtgt attgcagaaa agctaaatct
     9961 tccggcacca gttcgggttt gacgtactgc atccggtagc cgttatgcag tgcgctacga
    10021 aatgaaatgg catctggcag atggtatttc ggcaccaaac gcttgatgta tttaacaacg
    10081 aaattgacta ccgtgccttt tgccgtagat agctgatcgc ccatacgggt cagaattacg
    10141 tgctgaacgg cggttttatc aaccactttt tccagtgtgt gagcaaagtt agacacgata
    10201 acaatcgccg atgcgccgct atcgttaagc tgatactcaa gctcacgcgg ggtatacaac
    10261 gggttaacgt ttacgacgat catcccggca cgcaaaatgc caaacagcgc caccggatat
    10321 tgcaataaat taggcatcat caacgcaacg cgatcgcctt tcttcagccc caacccttgt
    10381 tgcaaataag cggcaaacgc gcgactgcgt tcttccagct tgcggaaggt cattacctcc
    10441 cccatattca caaacgcagg ttgatcggcg tagcgcgcga ccgactgctc aaacatatct
    10501 accagagatt gataacggtc agggttgatc tccgtcggaa cgtccgcggg ataacggtta
    10561 agccaaacct tcttcaattc ttcacctcta aaatgcgtgt tcgtcgtcat cgcaacccca
    10621 aatgatatac atgccgttaa cataatatta actcatcata ccagcttgat aattacccaa
    10681 cgaaaaggtt gcgaagcgcg tcactattta tttttatctt taccgtaaga atgcagaaac
    10741 agcggaccag ccgctgtttc tttttcttta aaaacaagca attattcagt tacaactgtt
    10801 tgtactctcg cggggccggg attataccag ccccatccgc cgtagccata gggccagcca
    10861 cgaccgccat aaaaccatgg atcaatcggc tgaggcggca taatcacctg ctgggttaaa
    10921 tgccaacgtt tgtaacccgt tacttgcatc accataaatt tatagggcgt attgccgatt
    10981 ttgccgtcaa ccgcaccagt gattggcccg actaccgtaa caagttgtcc acggaaatcc
    11041 accgggtcca gaaaaccgtt cacatcggca taaatgcgac cgcgagaagg ttctcccagc
    11101 gtcggtctgg ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt tttcccttgc
    11161 tggttttgta ccgcaaccac tttgccacca aagcgtgcct cctgaccaac gtacagctgc
    11221 ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg gactgctgcc tttaatggcg
    11281 tccggcacag tgacacaacc gctcagcatt agcgcaaacg tacctgccag tatgcctttg
    11341 ataacattct tttgaaccgc catgatgcga ctcctttttt tctcagggca tactcttaag
    11401 attcattctt tgcccggaag tttcttccat gcgacgttgt tacgtaaata aaccggttcg
    11461 gcatgttcca ccgccaccgt tttaccctcg gcaaacatct gacacgcaat cggcagcata
    11521 tcttccgccg ctggcagtaa aacttctccg tcgcgcaaaa ccagcccgct ctctttaccg
    11581 agatccggcc aggcttgcca gcccgtccct accgttaccc attcgccgga aagctgttgc
    11641 attcgttcat gaacgagttc gggttttagt acggcttcgg tttcttcacc gcgccagata
    11701 ccgttttcat cacgctgata ttcggcccag taaacttcgc ccattcgcgc gtcaatggct
    11761 gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccatcgt cattagcgtg
    11821 gagacgccaa tcatcggtag ctctgcgcca agtgccagcc cttgcgcaat gccaatacca
    11881 atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt aatatcagtc
    11941 agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg ttgagtatgt
    12001 tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag ggcggcagag
    12061 caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc ttagatcaat
    12121 aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg tatcgcaagg
    12181 aaacgaaccg cacgggcaat gtcacgagtg cgtggcgcgg gcggcagact ggcgagaaac
    12241 gtcgcgccgt aaggacgcat caccagccga ttgtcacaaa tcaccagcac gccacgatcg
    12301 tcggcgtcgc gaatcagtcg ccctaccccc tgtttgagag taatgacggc atctggtagt
    12361 tgcacttcat cgaacgggtc gccaccgcgc aaacgacaat cttccatgcg cgcttttaac
    12421 agtggatcat ccggcgaggt aaacggcaac ttgtcgataa tcaccaatga caatgtatcg
    12481 ccacgcacgt ccaccccttc ccagaaacta ctggttgcca caagaagcgc gttaccggcg
    12541 ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa aacgggaagc
    12601 gtcatggtag cgcggaactg ctcggccaga tcacgcatca tggcgtgcga ggtacaaagc
    12661 ataaaacaac gaccgttgtt agcttcgatg atcggtcgca gcattgccgc taactggcga
    12721 gcagaacctg gctggttggt ttgcggcaga ttgcgcggca cacagagtaa cgcctggcgg
    12781 ctgtaatcaa acgggcttgg caatagcaac gactccgcct gttctatccc aagccgcgag
    12841 gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtgaagat ccagctacca
    12901 ggcttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt gagagccaga
    12961 gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt gatctctttt
    13021 agccgcttca gccgtgtgcg atacaacgta gcgcgctcaa atgccgcatc cagcaaggcg
    13081 gaacgcccca gtgacagttt cgccacgtca taacaaagtt ccagggtgtc atcgagcagt
    13141 aaaaatgccc gctgaatttg cggattagct aacagctcac gcaggttacc gcgataacct
    13201 ggctcaccga gttgcagacg aaaatcctgc gcactctggg caagacgatc agcgcacttt
    13261 tgtaactgct gggtgtcttt taattcggtg cggtaagcga tggtgatgtc ttttgccagg
    13321 tcgagcagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat gtccggtagc
    13381 tggtgggctt cgtcgaagat catgacgtcc gcttccggga tcagttcgcc aaatccactc
    13441 tctttaacca ccatatccgc cagaaagaga tgatggttta ccaccaccac atcggcgtcc
    13501 atcgcttttt tacgtgcttt gaccacaaag caatctttat acatcgggca gtcgctgcca
    13561 agacagttgt cgttggtgct ggtgaccagc ggccacgcct gtgaatcttc cgccacgctg
    13621 acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag caggatcaca
    13681 tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc gagacgctcg
    13741 aggcagaggt agtttgagcg ccctttcagc agcgccacgt tgcccgtata tttcaatgcc
    13801 tttgagactg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt tgagccggtc
    13861 gagataatga ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta ggttttgccc
    13921 gtaccggttc ctgcttccac caccagcggc tggccttttt ctatcgcctg ggtgacggct
    13981 accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga
    14041 ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacag ggattatgtc
    14101 aggatgaggg cgctttcgcc agttgaagtg gtgacgacga cctcacattg tggcagtctt
    14161 tgcagcacga aaatggaagg ataatgagga caaaatgatg actatcgttc gtatcgatgc
    14221 tgaagcccgc tggtctgatg tggtaatcca caacaacacg ctctactaca ctggtgtacc
    14281 ggaaaacctc gacgccgatg cctttgagca aaccgccaac acgctggcgc agattgatgc
    14341 cgtgctggaa aaacagggca gcaataaatc gagcattctg gatgccacca ttttcctggc
    14401 cgataaaaac gacttcgcgg cgatgaataa agcgtgggat gcctgggttg tcgcgggtca
    14461 tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac ccgaagtata aagttgaaat
    14521 taagattgtg gctgcggtgt aagctttatc gaagcaaaat aagtcagacg ataatttatc
    14581 gataatactg gtcgctttta cataaatcga ccagagatta agatatta
//
LOCUS       DAZUFB010000118        14537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZUFB010000118 DAZUFB010000000
VERSION     DAZUFB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.118"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(79..1329)
                     /gene="icd"
                     /locus_tag="ACHY3M_004072"
     CDS             complement(79..1329)
                     /gene="icd"
                     /locus_tag="ACHY3M_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFS4318888.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1501..2154
                     /gene="rluE"
                     /locus_tag="ACHY3M_004073"
     CDS             1501..2154
                     /gene="rluE"
                     /locus_tag="ACHY3M_004073"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFS4318889.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2164..2625
                     /gene="nudJ"
                     /locus_tag="ACHY3M_004074"
     CDS             2164..2625
                     /gene="nudJ"
                     /locus_tag="ACHY3M_004074"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFS4318890.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2679..3785
                     /gene="mnmA"
                     /locus_tag="ACHY3M_004075"
                     /gene_synonym="trmU"
     CDS             2679..3785
                     /gene="mnmA"
                     /locus_tag="ACHY3M_004075"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4318891.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3821..4462
                     /gene="hflD"
                     /locus_tag="ACHY3M_004076"
     CDS             3821..4462
                     /gene="hflD"
                     /locus_tag="ACHY3M_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFS4318892.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4466..5836
                     /gene="purB"
                     /locus_tag="ACHY3M_004077"
     CDS             4466..5836
                     /gene="purB"
                     /locus_tag="ACHY3M_004077"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4318893.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6004..6675
                     /gene="phoP"
                     /locus_tag="ACHY3M_004078"
     CDS             6004..6675
                     /gene="phoP"
                     /locus_tag="ACHY3M_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFS4318894.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6675..8135
                     /gene="phoQ"
                     /locus_tag="ACHY3M_004079"
     CDS             6675..8135
                     /gene="phoQ"
                     /locus_tag="ACHY3M_004079"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFS4318895.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8211..9332
                     /gene="roxA"
                     /locus_tag="ACHY3M_004080"
                     /gene_synonym="ycfD"
     CDS             8211..9332
                     /gene="roxA"
                     /locus_tag="ACHY3M_004080"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFS4318896.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9478..10707)
                     /gene="pepT"
                     /locus_tag="ACHY3M_004081"
     CDS             complement(9478..10707)
                     /gene="pepT"
                     /locus_tag="ACHY3M_004081"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4318897.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10957..12093
                     /gene="potA"
                     /locus_tag="ACHY3M_004082"
     CDS             10957..12093
                     /gene="potA"
                     /locus_tag="ACHY3M_004082"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFS4318898.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12077..12940
                     /gene="potB"
                     /locus_tag="ACHY3M_004083"
     CDS             12077..12940
                     /gene="potB"
                     /locus_tag="ACHY3M_004083"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFS4318899.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            13304..>14537
                     /gene="espK"
                     /locus_tag="ACHY3M_004084"
     CDS             13304..>14537
                     /gene="espK"
                     /locus_tag="ACHY3M_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFS4318900.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLRVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSDG
                     YGQSITASELRWIYRNKDN"
BASE COUNT         3702 a         3640 c         3567 g         3628 t
ORIGIN      
        1 tatgctatta ataataccta cataaataaa acaacgggcg tgttatacgc ccgttgcaat
       61 attaaacaca tgtagtgatt acatgttctt gatgatcgca tcaccaaatt ctgaacattt
      121 cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct tcgcattgat
      181 tgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca tatggcgtaa
      241 catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac cggcgtattt
      301 cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt ttgctccagg
      361 ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt caccgttcag
      421 gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt gcaggaatgc
      481 atcagcaatc acgtctttaa tgacgatctc tttgccggtg ttcgggtttt taactttcag
      541 ccacgggccg ccgtcgatca gttcaccgcc aaactcttca cgcgccagct ggtagcccca
      601 gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca gagtcacaga
      661 gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt tggtgccttc
      721 ttccgaacac ggcttaatac cgataccaca atgttccggg aagcgaattt tcttcacccc
      781 catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtctg ctttccattc
      841 gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg tcagttcagg
      901 gtgtttaacc gggcttggag tgccttgata gtaacgtacc ggacgcaggc agatgtagag
      961 atccagttcc tggcgcaggg caacgttcag agagcgaata ccgccaccaa caggagtggt
     1021 cagcggacct ttaatggcaa cgcgatattc acgaatcaga tcaagagttt cagcaggcag
     1081 ccagacgtcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt ccatccagga
     1141 gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt tcagcatggc
     1201 tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg gattttcagg
     1261 aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa caactacttt
     1321 actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac cacgttgcat
     1381 atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat gatttgtaat
     1441 tggcttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta ttaggctata
     1501 atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag aaatcaccag
     1561 gttaagcgat tcagctcgca acgttctacc aggcgtaaac ctgaaaacca gcccacgcgt
     1621 gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga agccggacgc
     1681 aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc
     1741 gatagcgaag ggttactggt gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag
     1801 ccgggcaaac gcaccgggaa aatctattat gtgcaggtgg aaggtattcc cacacaagac
     1861 gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct gcccgcaggc
     1921 gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat tcgtgaacgc
     1981 aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa tcgccaggtg
     2041 cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta tgcgatggga
     2101 gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga ttaaggatgc
     2161 aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg caaattttta
     2221 gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg gcatctggaa
     2281 gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac cggcatcagc
     2341 gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa aacgccgttt
     2401 ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc tcatgacagc
     2461 gatatcgact gctgtcgttg ggtcagcgcc gaagaaattt tacaggcgtc aaatcttcgt
     2521 tcgccgctgg tggcggaaag tatccgttgt tatcaaagcg ggcaacgcta tccgctggag
     2581 atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc tgcgtgatag
     2641 aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag
     2701 taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac
     2761 agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac ggtgaggaat
     2821 attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag ctcggcattg
     2881 aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa ctgttccttg
     2941 ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa gagatcaaat
     3001 ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc gctaccggtc
     3061 attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt ctggacagca
     3121 ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg caaagcctgt
     3181 tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg
     3241 tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt aaattccgcg
     3301 agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg
     3361 aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta
     3421 tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa
     3481 acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct gtcgggttga
     3541 ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggcactatg cgttgcacgg
     3601 taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg gacgatgatc
     3661 gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag tctgccgtct
     3721 tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg ccgctgccgg
     3781 tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga attactatga
     3841 catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac aactcgctca
     3901 ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta ttgatatgaa
     3961 ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga
     4021 aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgccg aattaacccg
     4081 ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga
     4141 cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg atttacagtc
     4201 cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc cgcttggccc
     4261 gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt
     4321 tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg
     4381 acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac aaattcttgc
     4441 tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg tttcccctgt
     4501 cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg aatatggttt
     4561 gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc
     4621 gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg caattgtcgc
     4681 caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca ctaaccacga
     4741 cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc
     4801 ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc tctcccacgc
     4861 attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc aactgattga
     4921 tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc gtacccacgg
     4981 tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct accgtatgga
     5041 gcgccagtac cgccagctta atcaggtgga gatcctcggc aaaatcaacg gcgcggtcgg
     5101 taactataac gcccatatcg ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga
     5161 gttcgtcacc tcgctgggta ttcagtggaa cccgtacacc acccagatcg aaccgcacga
     5221 ctacattgcc gaattgtttg attgcgttgc gcgcttcaac accattctga tcgactttga
     5281 ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga
     5341 gattggttct tccaccatgc cgcataaagt taacccgatt gacttcgaaa actccgaagg
     5401 gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc cggtttcccg
     5461 ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta
     5521 tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag taaaccgtga
     5581 ccatctgctg gatgagctgg atcacaactg ggaagtgctg gctgagccaa tccagacagt
     5641 tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa
     5701 gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc cagaagaaga
     5761 gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta tcaccatggt
     5821 tgatgagctg aaataaactc gtatcagtgc cggatggcga tgctgtccgg cctgtttatt
     5881 aaaattatcc actttttctt ttttcacttt acctcccctc cccgctggtt tatttaatgt
     5941 ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca gggagaaata
     6001 aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct taaagttcag
     6061 attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc cgattattat
     6121 ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga cgaggacggt
     6181 ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac tgccgattct ggtattaacc
     6241 gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga tgattacgtg
     6301 actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat gcggcgtaat
     6361 agcggcctgg cttcacaggt catttcgcta cccccgtttc aggttgatct ctctcgccgt
     6421 gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac cattatggaa
     6481 acgttgatac gcaataatgg caaagtggtg agcaaagatt cgttaatgct ccaactctat
     6541 ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg tctgcgcaaa
     6601 aaaattcagg cacaatatcc gcaagaagtg attactaccg ttcgcggcca gggctatttg
     6661 ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctca ctgcgggtac
     6721 gttttctgtt ggcaacggct gcggtagtac tggtgctttc gcttgcctac ggaatggtcg
     6781 cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta cgtggcgaga
     6841 gcaatctgtt ctataccctt gcgaagtggg aaaacaataa gttgcatgtc gagttacccg
     6901 aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac gggcagcttt
     6961 tatgggcgca acgtgacgtg ccctggctga tgaagatgat ccagcctgac tggctgaaat
     7021 cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg ctgagtggag
     7081 atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac gcggagatga
     7141 cccactcggt ggcagtaaac gtctacccgg caacatcgcg gatgccaaaa ttaaccattg
     7201 tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg agctggttta
     7261 tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc gccgcctggt
     7321 ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa gaacataacc
     7381 gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga aacctgaacc
     7441 gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc accgacctga
     7501 cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct ctgcgtagtg
     7561 aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc cgcatttcac
     7621 agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg ctcagccgcg
     7681 agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac aaagtgtatc
     7741 aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt gtcggtgagc
     7801 agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa tattgcctcg
     7861 agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg gtcgaggatg
     7921 atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt caacgggttg
     7981 atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc accgagcaat
     8041 atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg gaggtgattt
     8101 ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccatacttc acgcattacg
     8161 ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac atggaatacc
     8221 aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc ccggtggtgt
     8281 taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg gcgggtctgg
     8341 cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa tggcaggtca
     8401 gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca ttactggtac
     8461 aggcagtgaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac
     8521 cggactggcg tattgatgat ctgatgatct ctttttctgt accgggcggc ggcgtcggtc
     8581 cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggtcgtcgt cgctggcgag
     8641 tgggcgaaaa gctgcaaatg aaacagcact gtccgcaccc ggatctgtta caggtcgatc
     8701 cgttcgaagc catcatcgat gaagagctgg agcctggcga tattctttat attccgccag
     8761 gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg
     8821 cgccaaatac gcgggaatta attagcggat ttgccgatta tgtgctgcaa cgtgaactgg
     8881 gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg gacgttctgc
     8941 cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag ccggaacact
     9001 ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat atcgcgccgc
     9061 cggaaccgcc ttatcagcca gatgaaatct acgatgcgct gaaacaaggt gaagtgctgg
     9121 tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga
     9181 agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt gcgctgactg
     9241 cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg
     9301 tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga tgcggcgctg
     9361 attgcgcctt atcgtgccta catgcactga acgcctgtag gcacgataag tatgtggacc
     9421 agtatcaata taacggtttc atcccggtaa ttggcagtcc acatacgcat cgggcaatca
     9481 cgacttccgt tgcgccgtta actcggcaat acggacaatc acctgcaccg ctttttccat
     9541 accttccaga gtcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt
     9601 cgggcacggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt
     9661 cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga taatatgcgg
     9721 atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca gttcaatgta
     9781 gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg
     9841 cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg ctcggtcaac
     9901 ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct
     9961 ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac
    10021 tcctttcgcc gtgcccggat gaacattgtt accgacaatt ttgatattga ccgatgcggc
    10081 gttgaagttt tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg
    10141 ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt
    10201 aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt
    10261 catgatttct gcaatacctg ctttgtcatc ggcacctaac aaggttttac catcggtggt
    10321 aatcaacgtc tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc
    10381 atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattcac
    10441 atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat
    10501 atcgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac
    10561 attgataagc cccatctctt cgagctgctc tttcagcaga tgcaataact tccattggcc
    10621 ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt
    10681 caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt
    10741 aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat
    10801 ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca aagattggcg
    10861 attcaagcgg tttgccgtag aattaccgcc ctcattaaga gtcaccaagg tggttaacca
    10921 caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat
    10981 aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat
    11041 ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg
    11101 cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact
    11161 gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac
    11221 cgctatgtga acactgtttt ccaaagctac gcacttttcc cccacatgac cgtgttcgaa
    11281 aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg
    11341 atggaagccc tgcggatggt gcaactggaa accttcgctc aacgtaaacc gcatcagctc
    11401 tctggtggtc aacagcaacg cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg
    11461 ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac
    11521 gagctgaaag cgttacagcg taagcttggt attaccttcg tctttgtcac tcacgatcag
    11581 gaagaagcgc tcaccatgtc agacaggatt gtggtgatgc gcgagggtcg tattgagcaa
    11641 gatggtacac cgcgcgaaat ttacgaagag ccgaaaaacc tgtttgttgc cggcttcatt
    11701 ggcgaaatca atatgtttaa cgccactgtc atcgaacgtc tagacgagca gcgcgtacgc
    11761 gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa
    11821 aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac
    11881 cacgctgaag ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag
    11941 tcggttgttg aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac
    12001 gatcctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg
    12061 gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat
    12121 tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt
    12181 tttgacccgc gacgacgcca gtttcgtaaa aatggtcttt acgctggata actacacgcg
    12241 tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc
    12301 caccctcgcc tgcctggtgc tgggctaccc gtttgcctgg tttctggcga agctgccaca
    12361 caaggtgcgt ccgctgctgc tgtttctgct gattgttccc ttctggacca actcattgat
    12421 tcgtatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct
    12481 ctggctgggc gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat
    12541 cggtctggtt tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa
    12601 actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt
    12661 tatccgtatc attattccac tgacgatgcc gggaattatt gccggatgtc tgctggtgat
    12721 gctgccagcg atgggcctgt tctatgtatc cgacctgatg ggcggtgcga aaaacctgct
    12781 gatcggtaac gtcatcaagg tacagttcct caatattcgt gactggccat ttggtgcagc
    12841 caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc
    12901 tcgtctgctg aataagaagg caacagaaat agataattag tgctttatat tcaaaaggtt
    12961 aataggaaat ttggcatgct ttttactgtc tagtaaacag catgcctcta tccataaaat
    13021 caaacagttg caatctaatt ttggagaacg ttttttcttc tcccttttag caaatcgcta
    13081 taaaaaaaca acaaatggta taaaaatgag caatcctaat ccatgcatga cggactggag
    13141 aaagtattcg caaaagttaa aatcaacagt ctgttggttt tgagatataa ctctcttttc
    13201 taatattgac accttggctt tgctggttgt taaaattagc ttgttgatta gtagtatgga
    13261 tagaacttac tctgttattt atttctaaaa atggaattta tcaatgcttc ctacatcgca
    13321 attacgaccg accgggacat tctgctccta ttccgctgaa acatcagcag acatcaaaag
    13381 cgaaatcaca ccaattcaga tagaagaagc gcgggccagt ggtcgtttat atatcaaaga
    13441 ttgtgatatt gagtatctgc cacagttacc aaacgaaata acatcagtta caatcgaaaa
    13501 ctgcaacaac ctgacaaccc ttacaggatt gccggttaat acacaaaacc tctccgtcat
    13561 taactgtgaa aaattacaaa tcacagacat gccatcaacc gtaaaaaatc tacatattga
    13621 attaactgat tcaccattta tacatttcat atctgaaggc atcgagtgcc tgagggtttg
    13681 ccactgctat atatctggag tgccagagag tgtccgctac cttgaaataa aaggtagcgc
    13741 cacagacagc ataaaaaatg ttccaaacgg gttatcatct ctcagcatca atagctataa
    13801 tccggagaat caggccagaa ttgacaacct gatatcaccg tcactgaaga cgctatcgct
    13861 gactggatgt agcaatatta tactgccgga gaaacttccg gagagtgtga catcggtaac
    13921 cattcatgcg gagcagaaaa ccacgtggaa catcggtgtt gaagggatgc ctgatgggct
    13981 ggatcttgat ttacaaaatg tactactctc tccagatgta gttaaagcaa aaaacatcac
    14041 ctttcagggc aacgctctgg atgtggcctt acactttcgc gagggagaca ttgtctatgg
    14101 actatcttca cccagagaaa aacttgtcaa cagcattaaa ctagttaacg acttttccaa
    14161 aaaagatatt ataactcaga atacgttaac aaacgcagta tgggacccca gaacacctcg
    14221 caaatataag caagatccac ttatcaaaag agcattaaat gaacacgaaa gagggataaa
    14281 atttaaacaa cacttaaaga atcacaataa ttataatgtt accatggccg acctttccgt
    14341 atacaatcgc gacaaattat gggcaaaaac aagcaaggcc ggcctagagt ttcagacatt
    14401 aacacgcaat aaaacggtta ttttttgtgc ggatgagctt gtcaactcac tcaaactcat
    14461 agctaacaag tcagatggct atggccagag tattaccgcc agcgaattac gatggattta
    14521 ccgtaataaa gacaaca
//
LOCUS       DAZUFB010000119        14476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZUFB010000119 DAZUFB010000000
VERSION     DAZUFB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.119"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..641
                     /locus_tag="ACHY3M_004085"
     CDS             75..641
                     /locus_tag="ACHY3M_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318901.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            692..3151
                     /locus_tag="ACHY3M_004086"
     CDS             692..3151
                     /locus_tag="ACHY3M_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4318902.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3166..3897
                     /locus_tag="ACHY3M_004087"
     CDS             3166..3897
                     /locus_tag="ACHY3M_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4318903.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3894..4955
                     /locus_tag="ACHY3M_004088"
     CDS             3894..4955
                     /locus_tag="ACHY3M_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4318904.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5107..6153
                     /locus_tag="ACHY3M_004089"
     CDS             5107..6153
                     /locus_tag="ACHY3M_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4318905.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6150..6941)
                     /gene="nei"
                     /locus_tag="ACHY3M_004090"
     CDS             complement(6150..6941)
                     /gene="nei"
                     /locus_tag="ACHY3M_004090"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFS4318906.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6977..7711)
                     /gene="pxpA"
                     /locus_tag="ACHY3M_004091"
     CDS             complement(6977..7711)
                     /gene="pxpA"
                     /locus_tag="ACHY3M_004091"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFS4318907.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7701..8633)
                     /gene="pxpC"
                     /locus_tag="ACHY3M_004092"
     CDS             complement(7701..8633)
                     /gene="pxpC"
                     /locus_tag="ACHY3M_004092"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFS4318908.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8627..9283)
                     /gene="pxpB"
                     /locus_tag="ACHY3M_004093"
     CDS             complement(8627..9283)
                     /gene="pxpB"
                     /locus_tag="ACHY3M_004093"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFS4318909.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9306..10049)
                     /gene="ybgI"
                     /locus_tag="ACHY3M_004094"
     CDS             complement(9306..10049)
                     /gene="ybgI"
                     /locus_tag="ACHY3M_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFS4318910.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10320..11801
                     /gene="dtpD"
                     /locus_tag="ACHY3M_004095"
     CDS             10320..11801
                     /gene="dtpD"
                     /locus_tag="ACHY3M_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFS4318911.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11843..13261)
                     /gene="phrB"
                     /locus_tag="ACHY3M_004096"
     CDS             complement(11843..13261)
                     /gene="phrB"
                     /locus_tag="ACHY3M_004096"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFS4318912.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13258..13767)
                     /locus_tag="ACHY3M_004097"
     CDS             complement(13258..13767)
                     /locus_tag="ACHY3M_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFS4318913.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13918..14476)
                     /locus_tag="ACHY3M_004098"
                     /pseudo
     CDS             complement(13918..14476)
                     /locus_tag="ACHY3M_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3495 a         3864 c         3512 g         3605 t
ORIGIN      
        1 taaaaacatt cgacaatcaa ataagatatt cttgcaccga tatacttttt tcttttctgt
       61 taaaggactc cgttatgttt aaaggacaaa aaacattggc cgcactggcc gtatctctgc
      121 tgttcacagc acctgtttat gcggctgatg aaggatccgg tgaaattcac tttaaaggtg
      181 aagttattga agcaccgtgt gaaatacatc aggatgatat tgataaagag gttgaactcg
      241 gtcaggtgac caccagccac attaatcagt cacatcacag cgatgccgtt gctgtcgacc
      301 tgcgcttagt caactgtgat ctggaaaact ccagcaacgg ttccggtggc aagatttcta
      361 aagttgcagt cacctttgat agctcagcga aaaccaccgg cgcagatccg attctcaaca
      421 acaccagcac gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa gatcaaagca
      481 atatcgtttt aggcaccgct actccagata tcgacctggc tccgacctcc agcgaacaaa
      541 cgctgaattt ctttgcctgg atggaacaaa ttgatcaggc tacacctgta acgccaggcg
      601 cagttacggc gaatgcaacc tacgtgctgg attataaata agtattactt tggcggcgct
      661 atcgccgcct ttcataaaat atttatcagg tatggacaca gtgaatattt atcgactctc
      721 ttttgtatcc tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat tcaaccttaa
      781 tgttctcgat aaatcaatgc gcgaccgcat tgatatttca ttattaaagg aaaaaggagt
      841 cattgctccc ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa tcagtaacgg
      901 gcaaaagatt aactggcaca aaaatgacga taaaaccatt ccgtgcatca atgatttact
      961 ggtcgataaa tttggcttaa aacctgaagt ccgtcagtcg ttaccattga taaatcagtg
     1021 cgtcgatttt agctcccgac ctgaaatgct cttcaatttc gatcaagcca atcagcaact
     1081 aaatatcacc attccgcaag cctggctggc gtggcactca gaaaactgga ccccaccctc
     1141 cacatggaaa gaaggtgtcg ccggtgtcct gatggattac aacttgtttg ccagcagcta
     1201 ccgcccacag gatggcagca gcagcactaa tcttaacgcc tatggaacag ccggaatcaa
     1261 cacgggggca tggcgcttac gcagtgatta tcagttgaat cagactgata gcgatgataa
     1321 ccatgaacag tcaggcgaaa tatcacgtac ctatcttttt cgcccgttac cgcaattagg
     1381 ttcaaaatta accctcggcg aaacggattt tagttccaat attttcgacg gtttttctta
     1441 taccggcgcg gcactggcaa gtgacgatcg aatgttgcca tgggaactac gcggctacgc
     1501 cccacaaatt agcggtattg cacagaccaa tgccacggtg acgatcagtc aatcaggccg
     1561 cgtcatttac cagaaaaaag tcccaccagg cccatttatc attgacgacc ttaatcagtc
     1621 tgttcagggc acactggatg tcaaagtgac ggaagaagat ggtcgggtga acaatttcca
     1681 ggtttcggca gcatcgacgc ccttcctgac tcgtcaggga caggttcgct ataaactggc
     1741 cgcgggtcag ccacggccct ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa
     1801 tgaagtttcc tgggggatgc tgtcaaacac ctcgctgtac ggcggcctgc tgctttctgg
     1861 tgatgactac cattctgccg caataggtat cgggcaaaat atgctgtggc ttggcgcgct
     1921 gtcgtttgat gtcacgtggg ccagtagtca ctttgatact cagcaggacg agcggggctt
     1981 aagctaccgt tttaattaca gcaaacaagt ggatgccacc aacagcacga tttccctcgc
     2041 cgcttatcgt ttctccgatc gtcattttca cagctacgcc aactatcttg atcacaaata
     2101 caacgacagc gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat
     2161 taccccacta aacctcaatc tttacgccaa cctgctacat caaacctggt ggaatgcaga
     2221 cgcctccacg accgccaaca tcacggcagg ttttaatgtt gatattggtg actggagaga
     2281 tatctctatt tcgacgtcat tcaatacaac ccattacgaa gataaagatc gcgacaacca
     2341 gatttacctg tcgatttcgc tccccttcgg taacggtggt cgggttggct atgacatgca
     2401 aaacagtagc cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa
     2461 tagctggggc atgtctgccg gactgcaatc cgaccgtccg gacaatggag cccaggtgag
     2521 cggtaactat cagcatctga gttcagcggg tgagtgggat atttctggta cctatgccgc
     2581 caatgattac agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg
     2641 tgcagcgttt catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg
     2701 cgtggcagat attccggttc agggcaatct cgactacacc aaccattttg gcattgcggt
     2761 ggtgccgttg atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc
     2821 cgacggcgta acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg
     2881 ttacaaatca ctggcttccc gttccggtaa agacgttaac gtcatcattc gcaacgccag
     2941 cggtcagttt cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg
     3001 gatggttggc gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaagtttac
     3061 cgtggtctgg ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac
     3121 cgcaaatcgc ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa
     3181 ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat
     3241 cgcactcgta ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt
     3301 agcgataaac tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc
     3361 gatgatcttc tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacgcag
     3421 gtacggatag taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt
     3481 ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg
     3541 caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa
     3601 gcaggagaaa aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg
     3661 aaaaatccta ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt
     3721 ctccccggct tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga
     3781 aattatagcg gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg
     3841 acgacgctca actgcagcgg acaatgccat ttacaagccg tggaggcgaa gaaatgagtg
     3901 ctggcaaggg agtgttgctc gtcatctgtc tgttatttct gccgttgaag tctgcgcttg
     3961 ccctgaactg ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac
     4021 cttttgctgt accaggcaat gccaaactcg gcgataagat ttgggaatct gacgatatta
     4081 aaattcccgt ctattgtgac aacaatacca acggcaattt tgaaagtgag catgtttatg
     4141 cctgggtaaa cccctacccg ggggtgcagg atcgctatta tcaactcggc gtaacttaca
     4201 atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtattg
     4261 acagtaagaa cattgacatt tatacccctg agcagattat cgcgatggga tggcagaaca
     4321 aaatttgctc gggtgatccc gctaatattc acatgtcacg tacctttctc gctcgcatgc
     4381 ggttatacgt caaaatacga gaaatgccgc cgcatgatta tcaaagtacg cttagcgact
     4441 atatcgtcgt gcaatttgac ggtgccggta gcgttaacga agatcctact gcccaaaacc
     4501 tgaaatatca tattaccggt ctggaaaaca ttcgcgtgct ggattgcagc gtcaattttt
     4561 ccatttcacc ggaaacacag gtgattgatt ttggtaaatt taatttgctg gatattcgtc
     4621 gccacacaat gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat gaccaatgca
     4681 ccgacggatt taaagtcagt tcctcctttt ataccgaaga aacgttggtt gaagaagata
     4741 aagcattgtt gattggtaat ggtctgaagc tacgtttgct ggatgaaaac gcctcgcctt
     4801 ataccttcaa taaatatgct gaatacgccg atttcaccag cgacatgttg gtctacgaaa
     4861 aaacctatac ggctgaactt tcgtccattg caggtacccc catcgaggct gggcccttcg
     4921 atacagtggt gctttttaaa attaactata actgatttgc catgatgccc tttgcagccg
     4981 gaaagggcat ctctttcaga aaattgctga ttttcccctt ttccttgatt gctgttattt
     5041 tttcctggcc cggctttctt tatgctgtat cgatacacca aagagaaaat aatgagggag
     5101 cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc tatcacttct cctttctatt
     5161 ggtttcctcg cggtacatct cccggcggcg ctattacttg ggccgatgat cgccgggatc
     5221 atcttcagta tgcgcggaat tactctgcaa cttccccgct ccgcttttct cgccgcgcag
     5281 gccattcttg gctgcatgat tgcgcaaaac ctcactggtt cgattctcac cacgctggcc
     5341 gccaactggc cgatcgtgct tgctatttta ctggtgacgc tgctttccag cgccatcgtg
     5401 ggttggttat tggtgcgcta tagctcactg cctggaaata ccggtgcctg gggttcttct
     5461 ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg
     5521 gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt tctggtcacc
     5581 cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg gttcccgcca
     5641 gtgagcatta atctcctgct tacgattttg ctcgcagtcg ttgccggtac ggcgggatgc
     5701 atgctgcgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag
     5761 tctggtcagc tcatcaccat cgaactcccg gaatggctgc ttgcgatggc gtatatggca
     5821 attggctggc ggattggtct tggtttcgat aaacaaatct tactgcgggc actacgcccg
     5881 ctaccgcaaa tcctgctgtc gatttttgct ctgttggcta tttgtgcggg tatggcgtgg
     5941 gggctgaccc ggtttatgca tattgatttt atgactgcct acctcgccac cagccccggc
     6001 gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc gcttattatg
     6061 gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg gacctgccat tgcacggttt
     6121 atttcaacct atgcgccgaa gcggtcggct tagtgctggc agccagggca ccagtaaaac
     6181 gggcgagatg acagcgtggt tttctcaatg atgctgccac aacgttcgca cggttcgcca
     6241 tctcgatgaa aaaccttaaa gcgaaacagc gccccatgat gcttattctc atccacctgc
     6301 ccccgcgtag cgtaagaaaa tcgaggaatc tccagtaacg cgtgtgcgag tgcatccagt
     6361 tgcgccgcat tgagatcttt cgctttatga tttccagtca acccaacctg ccagaggatc
     6421 tccacccgca aataattgcc aagtcctgcc agaaacgcct gctcgagcag taatcctgca
     6481 aactgacggt tacgaaagcg cggcgacaat acccgttctt tcaccacctc cggcgtcaga
     6541 ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc
     6601 ggggtcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc
     6661 agttttaccc gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag
     6721 acgccgtaaa gctgattatg gctgtagagc gttaattcgt tggaaaaatg agttaacaac
     6781 gccttaccac gcgtttccac atgggtaacg tgttgaccga taagttgtga ttgataagtt
     6841 tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc
     6901 aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat
     6961 tctttattaa gcggggttat gctgcgacaa caatcccctt tttggcaaat gtagctcgta
     7021 atcggcgggc gaacgccaga gcgtgctcgc cgtcgccatg caggcagacc gtttgcgccg
     7081 cgaccgttgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccagcg
     7141 tttgcgccag cgcctgttgt tcgttttcaa tcaacgcgcc tggctgactt cgcggcacca
     7201 gcgagccgtc agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt gtcaggccat
     7261 attgcttgcc tgcacgaatc agctcgcttc ctgccagccc aacgagaatc aatgccggat
     7321 cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcttctttc gccgcctggt
     7381 tgtacaacat gccgtgcggt ttgacatgac gcattacgcc gccttgcgca cgggcaatcg
     7441 ttgccagcgc gccaatttga tacagcgtct gggcgtaaac ggtttctggc ggcaactgca
     7501 tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca attgcaacac
     7561 cattttttat tgcttcacgc acgcaagcct gcatggtttg cgcatcgcct gcatgaaatc
     7621 cacaggcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt
     7681 cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa ttgttcaaaa
     7741 taacgttgct gatcttgccg cgctttcagt gcctcttcca gtgaacactg gacaaaatga
     7801 atcggctgac cgagcggaat ttgcgcaaga tggtacatat cagcctcaat gatgcaggca
     7861 atacgcgggt aaccaccggt ggtctgtgcg tcgttcatca acacaatcgg ctgcccgtta
     7921 tgtggcacct gcaccacgcc cggtaacaaa ccgtgagata acagttcacg atcggtggtg
     7981 cgttttaaaa tttgcccctg taagcgatag cccatgcggt tactttgcga gctaagctgc
     8041 cagggcgaac gccagaatgc atcctgcgag gcgcgatcga actcatgata ttccggcccc
     8101 ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa cgccctgcgc ttccataaaa
     8161 tcacgcttcg ctttgccaat cgggagtcgg tcaccatcct tcagtaaacg gccttccagc
     8221 ccgccaatcc ccactttgag atcggtgctg catgacccca ttaccggcgg aacatcaata
     8281 ccacccgcga ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc
     8341 tggcctgctt tcatcggcaa tcgccagccg gtccagacgg cattatcatc cagccgcgct
     8401 tcgcaaccgg cacccgtcag agcaaaccac ccatcagttt cgaactcaac agttaactga
     8461 ccgagcgtga tctccagcgc gggggcattg gcgtcattac ccaccagtag gttagcaatg
     8521 cgtaacgcgg gcatatccag tgcgccgcag tggctgatac ccgactggcg aaaaccgtga
     8581 cgaccgccat cctgcacagt ggtatacatg cccgcacgaa taatcttcag catactccct
     8641 ccttctgcgg tacaaagcgc acgctgtctc ccggacgtaa taagatgggt tcgtcacgcg
     8701 ccggatcaaa caggctgagt gaggtatgac caatcaactg ccagccaccc ggcgttgcca
     8761 gcggataaac accagtctgc ggcccgccga tcccgacaga acctgccgga acgagtaagc
     8821 gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga tacgggaagc
     8881 ccggttgaaa acctaaaaac cagaccacgt attccacgga ggagtgcaat tcaacaacct
     8941 gtttttcgct caacccgcaa tgcgccgcga ccaccgccaa atccggtccg cctgcaccac
     9001 cgtaaaccac cggaatttca ataaagcgag actcaggttc cagggcctcg ctctcctccc
     9061 accagcgttg caaacgctct atggcatcca gcgccagcga ctcaggatta cgcaaaatca
     9121 ccgtaatatt gttcatgccg ggaatggctt caaccacatt cggcatatcc accagacgct
     9181 gcgccagtcg ccagatccgt ttctggctag ccagcgtcac cggcggttcc agttccagca
     9241 ctaccgccgt ttcacctatc agataacaac gcgctcgttg cactttcgat ccctctgatt
     9301 attcgttatg caggattagg aatatcaata aaggtcacat caagatcggt attttcattc
     9361 agccactcgc tcaatgcgcg aataccacca cgttcagtgg catggtgacc tgcagcataa
     9421 aaatgcaatc cctgctcgcg ggctgaatga atggtctgtt cagaaacttc gccagtaata
     9481 aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc gcccgtgcac
     9541 caggcgacgc gctggaccac ctcaggtccg gtatcgccac accataacgg cttacgtccc
     9601 agacgcgctt caatccagga ggctagttcc agtcccggca caggcatggt cagctctccc
     9661 cacggcacca acggctcaat ttcgcccatg accgtgatcc ccagtaacgc cgccagctgt
     9721 gcgttattgc ccagctcagg atgtgcgtca agcggcaaat gccagccata caggttgata
     9781 tcattcgcca gcaacgtttt taaacggtta cgcttcattc cgcgaatgac cggagactct
     9841 cctttccaga agtagccatg atgcacaatg accgcatccg cgcccagacg cactgcctca
     9901 tcgagcaaag cctggctggc ggtgacaccg gtaacaattt tttgcaccgt ctctttgcct
     9961 tccacctgca aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta
    10021 atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg atagctacac
    10081 cgttaacata gcgttacctc gcctgttcgt cgatagccaa tcttttgaca tattaagaaa
    10141 caaacccgac gccaaaagac gcaaattgtt tatatcatca cgcttcagct aatgtatatt
    10201 tatgcaatca gacagcatat tgcataaata atcagatctg tatcaaagtt ttaactttaa
    10261 ggaaacacaa gcacgccgcg caactattac gtgacgttgc cgtcaattgt ggaattatta
    10321 tgaataaaca cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg caaatctggg
    10381 aatattttag cttttacggc atgcgtgccc tgctgattct ctatctcacc aatcaactaa
    10441 aatacaacga tactcacgcc tacgagttat ttagcgccta ctgttcgctg gtgtatgtca
    10501 cgccaatcct cggtggcttt ttggcggata aagttctcgg caatcgcatg gcggtgatgc
    10561 tgggggcgtt gttgatggcg atcggtcatg tggtgctggg tgccagtgag atccatccgt
    10621 cattcctcta tctgtccctg gcgattatcg tttgtggcta tggtctgttt aaatccaatg
    10681 tcagttgcct gctcggcgag ctgtatgagc caaccgatcc gcgtcgtgat ggcggtttct
    10741 cgctgatgta tgcagcgggt aacgtggggt ctattatcgc acctatcgcc tgtggttacg
    10801 cccaggaaga gtacagctgg gcgatgggct ttggcctggc ggcggtgggt atgatcgcgg
    10861 gtctggtcat tttcttatgt ggcaatcgtc atttcacaca tacccgcggc gttaataaaa
    10921 aagtactgcg tgcaacaaac tttctcctgc ctaactgggg atggctgctg gttctgctgg
    10981 tggcaacgcc tgcgctgatt accgtactgt tctggaaaga gtggtcggta tacgccttaa
    11041 ttgtcgcgac tattattggc ctgggtgtac tggcaaaaat ttatcgcaaa gcagaaaacc
    11101 agaaacagcg taaggagctg gggctgattg tgacgctcac cttcttcagt atgttgttct
    11161 gggccttcgc acaacagggc ggcagctcga ttagccttta tatcgaccgc ttcgttaacc
    11221 gcgatatgtt tggttatacc gttccgactg ctatgttcca gtcgattaat gccttcgcag
    11281 ttatgctgtg cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta
    11341 ccgtgcgcat ctggggaaaa tttgctcttg gccttggcct gatgagcgcc ggattctgca
    11401 ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg ccactgatgg
    11461 tattaggcct ggcggtgatg ggctttgcgg aactgtttat cgacccggtt gccatgtcgc
    11521 aaattacgcg cattgaaatt ccgggcgtga ccggcgtatt aaccggcatc tacatgctgc
    11581 tttctggcgc gatcgcgaac tatctggcgg gcgtgattgc cgatcagaca tcgcaggctt
    11641 cgtttgatgc ttccggggcg atcaactact ccatcaatgc atatattgaa gtatttgatc
    11701 aaattacctg gggcgcactg gcgtgtgtag gactggtact gatgatttgg ctgtatcagg
    11761 cgctgaaatt cagaaaccgc gcgctggcgc tggagtcata ataggccagc accatacctg
    11821 ataaaacgct cgctgcgttt tatcaggctc ctttcctcgc ttcttcgtac gctgccagcg
    11881 ttcgcagcct tgcctctttg tgatcaacta tcggttgcgg ataatccagc atcacacctg
    11941 ctttctgcgc ccacttccac ggctcatgca ccgctttccc tggtacatcg cgcagctccg
    12001 gtaaccatcg gcggataaac tcaccttcgc gatcaaattt ctcgccctgg gttgtcgggt
    12061 tgaaaatacg aaaatacggc gctgcatcag tacccgtaga cgccgcccac tgccagccac
    12121 cgttattggc tgccaaatca ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc
    12181 gccagtcgat caacaaatct ttcaccagaa aactggctga aatcatccgt agccggttat
    12241 gcatccagcc agtgctgtta agctgacgca tggcggcatc gacaatcggg tatcccgttt
    12301 tgccttcctg ccaggcctgt aaatgtgcgg gattgctctg ccactgcaca cgatccgtcc
    12361 aggcaataaa cggacaatgt ttacacaacg aggggtaata cgtcatcaga tgacggtaga
    12421 actcgcgcca gatcagctca ctaagccaga cactaccggc cccaccgtcc agcgcctgcg
    12481 gctgttccgc caacaagcga tgcaaacact ggcgaggcga taacccgccc gttgccaggc
    12541 tggcggacaa acgactggtg ccttccactg ccggaaaatc tcgttgttgc tcatattctc
    12601 cggcaccgtt ctggcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga
    12661 aatgcgcagt atcgaaagac tgacgaggat aattcagcgt aatggatggc gagggctcta
    12721 tcgatccgct actacgaact tttggcgcag cgacgcactc cggcatccct tcccgcagcc
    12781 gtttcagcca ggcattctta aaaggcgtaa agactttgta catctcgtga ttaccggtca
    12841 tcaccgcgcc aggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca
    12901 gcgctctttc aacttccaca tcccgcgccc gctcattcac ttcatactga tagttataaa
    12961 acaggtgggt aacgctgttt tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa
    13021 agtcatccac ttcacggaac aataaaggaa tacctttttc cgcaagcgct atttgtagcc
    13081 cattcagttg agcattgata agttcagcct gacgcggcga catgttatgc gtcgcccact
    13141 ggcgtggtgt agcgatatac aacgccagca cgcgtgcaga cgaattgcgg caggcggcag
    13201 ccagtgcgag attatcgtgc agacgtaaat cctggcgaaa ccagaccaga tgggtagtca
    13261 taaaactcct gaatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc
    13321 tgaaagccag gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag
    13381 aagtggttgc tcgccacggc gataactgtc gataagcgcc gccaactgct ggcgttctgt
    13441 ggaatcaata tgcgggcgaa aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg
    13501 cgggttggcg ggatgagaaa gcaaaacaat tacgcgttgg tagtactggt tgtaaaaatc
    13561 atccagattt tgccactggt gaatatcagc cacgaacggg ccaagtttgc ggtactccgg
    13621 ctgagaatgc gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg
    13681 cgttaagcca tgttctttca gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt
    13741 gctgtcatca aatcgttgct gattcattac tgctcctgaa cggtttacag gagtaagtgt
    13801 agacgttgag aaatgatatt tgtgaagtta atcctgttgt agcaggacaa atttacgaaa
    13861 gcctgctccc cgcaaggact gacgtcaggt agtttctaac catagctgcc ctttctattc
    13921 aaacgtgaaa tcaataagct catcagggac atcgcaaaca atatgaagac gggtttcttc
    13981 tctgccgtga ctcttttcac taattgcgta actgtcatgc ttgggattat ttaattcttt
    14041 cagcggaaat ttttcctcga aggctttatt tagccgcccc tggtttcctt ttacagcgaa
    14101 taaataatca cctccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc
    14161 atcggttgtg atgatttttc ctttaatatc cagcatgtta agaagttctg ggatagctgt
    14221 gatctcattg gatttcttat ccgtcttgat ctgcccgatg accagactgt gcattgttga
    14281 gaacgcacta atgacatgaa tcgctcccct gcggcgactc ttgtcataag agtgccggag
    14341 cgtttttcca tcaattgcaa tgacgtcttt atcatttgat gaatggcaat cacgcatcca
    14401 gttaataaag cactcgtgaa attttgcagg actgatacag gatacaactc tggcaatagt
    14461 atcgtgaaca ggaata
//
LOCUS       DAZUFB010000120        14475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZUFB010000120 DAZUFB010000000
VERSION     DAZUFB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.120"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..705
                     /locus_tag="ACHY3M_004099"
     CDS             <1..705
                     /locus_tag="ACHY3M_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFS4318914.1"
                     /translation="EGTVAISRDDFEDDNLGIYSPLFQEMGRAAAAQPDELVFAALGD
                     GISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQADYSGPAWYLLD
                     CSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGYGFWQMAYMMQS
                     PLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQLLERELFADGNATV
                     SNEMKGKLELVVADYL"
     gene            749..1108
                     /locus_tag="ACHY3M_004100"
     CDS             749..1108
                     /locus_tag="ACHY3M_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFS4318915.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAGQDA
                     PRKKTGNKAKQAKKARA"
     gene            1105..1524
                     /locus_tag="ACHY3M_004101"
     CDS             1105..1524
                     /locus_tag="ACHY3M_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFS4318916.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            1521..2081
                     /locus_tag="ACHY3M_004102"
     CDS             1521..2081
                     /locus_tag="ACHY3M_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFS4318917.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDGVLRSMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            2082..2327
                     /locus_tag="ACHY3M_004103"
     CDS             2082..2327
                     /locus_tag="ACHY3M_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFS4318918.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRLLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKSAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            2324..3823
                     /locus_tag="ACHY3M_004104"
     CDS             2324..3823
                     /locus_tag="ACHY3M_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFS4318919.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            3832..4197
                     /locus_tag="ACHY3M_004105"
     CDS             3832..4197
                     /locus_tag="ACHY3M_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFS4318920.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            4212..4688
                     /locus_tag="ACHY3M_004106"
     CDS             4212..4688
                     /locus_tag="ACHY3M_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFS4318921.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            4815..6890
                     /locus_tag="ACHY3M_004107"
     CDS             4815..6890
                     /locus_tag="ACHY3M_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4318922.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGMGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            6877..8226
                     /locus_tag="ACHY3M_004108"
     CDS             6877..8226
                     /locus_tag="ACHY3M_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFS4318923.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            8210..9334
                     /locus_tag="ACHY3M_004109"
     CDS             8210..9334
                     /locus_tag="ACHY3M_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFS4318924.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            9324..9938
                     /locus_tag="ACHY3M_004110"
     CDS             9324..9938
                     /locus_tag="ACHY3M_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS4318925.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            9931..10368
                     /locus_tag="ACHY3M_004111"
     CDS             9931..10368
                     /locus_tag="ACHY3M_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFS4318926.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GVATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            10368..11450
                     /locus_tag="ACHY3M_004112"
     CDS             10368..11450
                     /locus_tag="ACHY3M_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFS4318927.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            11441..12001
                     /locus_tag="ACHY3M_004113"
     CDS             11441..12001
                     /locus_tag="ACHY3M_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFS4318928.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            12001..12912
                     /locus_tag="ACHY3M_004114"
     CDS             12001..12912
                     /locus_tag="ACHY3M_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4318929.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(12947..13468)
                     /locus_tag="ACHY3M_004115"
     CDS             complement(12947..13468)
                     /locus_tag="ACHY3M_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4318930.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            13825..14010
                     /locus_tag="ACHY3M_004116"
     CDS             13825..14010
                     /locus_tag="ACHY3M_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318931.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGLSTSF"
     gene            14024..>14475
                     /locus_tag="ACHY3M_004117"
     CDS             14024..>14475
                     /locus_tag="ACHY3M_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4318932.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSFTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQV"
BASE COUNT         3253 a         3692 c         4322 g         3208 t
ORIGIN      
        1 gaaggcaccg tcgccattag ccgcgatgac ttcgaagacg acaatcttgg catctactcc
       61 ccgttgttcc aggagatggg gcgcgctgcg gcggcacaac cggatgaact ggtctttgct
      121 gcactgggtg acgggatcag tgcggcctgc tatgacggcc agaacttctt tgataccgaa
      181 cacccggttt accccaaagt ggatggctcc ggtgatgcgc agatggtcag caatatgttt
      241 gtggcaaaaa ccggctctgt cggtgcacag gcggattaca gcggtcctgc ctggtatctg
      301 ctcgactgtt cacgcgcaat caaaccgctg atttatcagg atcgccgtaa ggctgaactg
      361 gttgcccaga ccaaagtcga tgaaggtcgc gcattcaccg ataacgaatt tgtgttcggt
      421 gcttccgccc gtcgcaatgt gggctacggc ttctggcaga tggcctacat gatgcagtca
      481 ccgctgacgc tggatgcgct gtggcatggc tggtcagcca tgcgtgagtt taccgctgac
      541 ggtggccgca aactcggcat caaacccacc catattgttg tccccacctc gctggaaaaa
      601 caggcggtgc agctgctgga gcgtgaactg ttcgcagacg gaaacgcaac cgtctctaac
      661 gagatgaagg gcaagctgga gctggttgtc gcggattatc tgtaaacggt gagccgggtt
      721 tacggcccgg cccctgcgga gggcaaatat gaatgaacat cacactgtgg cagcaacaga
      781 tgattcaggt cttcaggtca gtggtgacaa ttctgtcacg gtgctggcag aagtgcgttg
      841 cagtcgttcg gcgtttcgtc gtgcggggtt cctgttcacg cgtggacgtc agcaggttga
      901 ggtcaccccg gagcagcttg cccgactgga ggcggagcca tgcctcactg tgcgaatact
      961 acagacgtct gctgatgatg cggggagcgt ggcgggtgtg gttcatgcag tggccggtac
     1021 agatttagcc gaagctgaag ccggacagga tgctccccgg aaaaagaccg gtaacaaagc
     1081 gaaacaggcg aaaaaggccc gcgcatgaat tacgccactg aaaccgatat gcgggcgcgt
     1141 taccgcgagg atttgctcag gccgttactg gctgtgcccc gttcggatga gccggacacg
     1201 cgcaagctga accgggcgct gacagatgcg tcagccctta tcgacagcta tctgtccgcc
     1261 cgttacacgt tgccgctgga ggttattccg gccgttcttg ttcagcactg ttgcgcgatt
     1321 gccttttatt acctgtgcga tcagcgagcc tccgatcagg cgcgtgaccg ttaccgtgaa
     1381 gcgctggcct ggctgaaaga tgtcatgaac ggcaatgtgc cggtcggtgt ggatacgaac
     1441 ggtgcggccc ctgaatccgg ggatttacca caggttcagt ctgatgcagc ggtattcggg
     1501 cgcaaccaga agggcttcat atgattacgg aaaccgaaca ggcatacatc gcccgtatcc
     1561 gtgagtattt cgggaatgaa cttgtgtctg ttgacacgca tcccggcgac tggagcgacg
     1621 gcgtactgcg cagcatgctg attaacgccc cggcgatcta cgttgcctgg ctgggtgccg
     1681 gtgaaggccg tacccgtggt cgcctggtca gtcactgggt gttctacgtc atcggcgaca
     1741 tgctcaacgg gcgtgaggcc agccgtcccg gactgtatca gattgtggcc cggctgattg
     1801 ccgtgcttaa cggcttcaga acagaaaaaa cctcaccgct ttactttgaa aaggcggtca
     1861 acggttacac cgaaacccag gcggacagtg gtgcggtgat gtatgcgctt tatttctcct
     1921 gcgaggaaat gatcgcaccg ctgaccgata tcagctcgct ggacgacttc ctgcgtcatt
     1981 acgaaacctt tgtggagccg cccggtacgc cgccgtttga ggcacatatt gatttgccgg
     2041 ggaacacgac aacggcggtc gaaccgccgg aggaactgta aatgaaaacc atctttatca
     2101 aacccgcgcc gggacgcctg attcgtgatc ccgacacaat gcgcctgctg gcacaggagg
     2161 gggaggaaaa gccctttacg ccgttctggt gtcggcgtct tgatgacggc gatgtcattc
     2221 aggctgaaaa atccgctgaa gaggctccgg ctgtatctgc ggatgcaacg actgcgacag
     2281 aaaaaccggt cgcccaaccg gcatccgata aggagaaacc gcaatgatta attttgacgg
     2341 gatcggtaat gaccgccgta taccgctgat tgaggttgag tttaataact ccatggctgt
     2401 caccggcact ccggcacagc gtcagtgtgt gctgctgttt ggtcaggcga gaatgaaaga
     2461 gaatgccgtg gacggtgccg gtgttctgga tgttccggtc aggatcaccc gtgcgtctca
     2521 ggcaacagaa ttctgggggc gcggctccat gattgccctg atggtggcgg aatttatcgc
     2581 catcaatcct gatgctgaac tgtatgccat tgctcagggg agcggcaccg gtcaggctca
     2641 ggcatgtctc atgaatataa ttggcaccgc ctctcaggat ggtgttctgc acatttatat
     2701 cggcggtcgg cgttatctgc tggctgtcac gaaagggcag aagggtaagg agcttattga
     2761 cgctctggtc tctgttatta atgccgatgc tgatgcgccc ttcagtgctt atgcaggtca
     2821 ggtagcagat aacgccagcg gactcaaggg aagtatggga attaatgccc gttttaccgg
     2881 tgaatgttcc gtacatgata tccgtgttaa ttatcatgac ggagaaacca caccgggtgg
     2941 actgaccatt gaacttgggt atcccaaaac aaaagccact aacccggata tcacccgaag
     3001 cgtggcgggt atgggcgatc gccagtacaa ctatgtcgtc atgccctata aggatgatgc
     3061 aaacctgaaa atcatcagtg atgagcttct gaaacgctgg ggaccggcca aaatgtctga
     3121 cggtgtgctg tggatggcgc ataccggcac gtttggtgag gtgcaggcat tcggggcaaa
     3181 acgcaatgat tttctggcga cctgcacatt cattcccaaa gcccctgaac cggattacct
     3241 ctgggcagcg gcggtctgcg ccacctgtgc gccgtctctg ggggcagatc ctgcgcgtcc
     3301 gcttcagacg ctggcgcttc cgtcacgcat ggccccggcc ccggccgatc gtctgacgcg
     3361 tgaagagcgt aacagtctgc tgtggggagg gattgccacg gtcagtgtgg ctgccggtga
     3421 tgtggttcag atcgaacgtc aggtgaccat gtatcgccag aacgtgtacg gcgaaagtga
     3481 tcccagctat ctggatgtgg aaaccatcta caccctctcc tggctgcgtt attccctgcg
     3541 tacttttatc acacaacgtt ttccgcgcca taaactggct gatgacggga cgcccgttcg
     3601 tgccggtcag aatatcgtga cgccggagat catgaagctt cagttaattt cgctgggtga
     3661 agagtgggtg gaactcgggc tggtcgaaaa tctggacacc tttaaaaaga atctgcttgt
     3721 ggagcgtaat gcgtcagatc gaaaccgtct tgacgtgtta tgcacgccgg atctggttaa
     3781 ccagttccgc ttcctggcag cacagatccg tttcattttg tgaggtaagg catgagcgga
     3841 aaacagtatc agggcacggc cactatccgt gtgaacggtc aggaatacga gacgcttgag
     3901 ggggctacat tttctccgtc aggttttgaa cgtgaagtgg tgaaaggcgc gaaagtctat
     3961 ggttaccgcc agaaaccacg tgaagcgacg ctggactgca aatttccggc aggcggagaa
     4021 ggttcaccag ccgccgatga aatcaacaac tggactgctg tcactatcga gtttgtggcg
     4081 gataccggcg aagtccacat gatgacgaag gcctggagca gtgaaccggc ctcgcttgac
     4141 ggtggcgggg agatctccgt gaagttcgcc agcgccacca gtacccgtgt tcagtgatca
     4201 ggaaaaataa aatgaccaca cgtaagaaaa aaacggcagt ttccgaggca gccgtgatgg
     4261 aggcaatccg ggaagcactt gagggggctg atccacgcac tgccgggctg acagagcaac
     4321 tggcaaaggg atatgtggat ctgcttgatg gtctgccgtt tggtgagacc cgtgaatatc
     4381 gtgtcacgtt ccgggaactg acagcgaaag acagtattga cgcagaagca gaagccgagc
     4441 gcgtggtgga gacaaacaat ggcccgatgc tgatagcgtc tccgtcgtta cgcggtgttg
     4501 cactgctgcg ccgtcagatt gccgcagtgg gagacattga aggtccgttg tcaccccgtc
     4561 agattggaca gttaagcgag cgcgatctct cccgcctgat ggcagcggtc agcctgctgg
     4621 ataccgcgct tgccggaaag ctggcagctg accgggggcg atcaggcgca gtgtcgggat
     4681 cagattgaag aagcggcaat tatcctgggg atggtgacaa aaagtggccc ggaatgggcg
     4741 cttaacctcc ccctgtcgca actttaccgg cactgccgac agacagaaaa aatcatcaga
     4801 acgaagcagt aagtatggcc cgaaatctca gagcatctct gatagttgat ttgctcggca
     4861 atatctcagc caaatcccgc cagtggtctc aggagctggg ggcattctca cgctccggcc
     4921 aggcggggct gggtggtctg ggaaatgcgg cccgccgtgc cgggcaggaa acagatgtac
     4981 tcggaagcag gatgcagcgc acgctggccg gggtgcgggg cagtatccgc catgtcactt
     5041 ctgattttga ccgtcttcag ggcagtatta ccggcaccat cgggcggatc agcaatcttt
     5101 acggaatgct ggccggtggt gctgctgtat atggctttaa ccggggattt attcgcccgg
     5161 cagcggagat ggaaacctat atgattcgcc tgaactccct ttacaaaggg gatcgggcga
     5221 aaactgatga tgtccgcagg tgggcaatac agaatgcgaa ggaaaccacc tgggggctgg
     5281 ctggcgtcat gcaggaatat acgtccagtc tgggctttgg tatgagtgac agggaggccc
     5341 gaaattttgt caccatgctt caggatcagg gtgccgtggg cggctggtca ctgtccgatg
     5401 cacagggggc atccctgcaa ctgaaacaga tgtatgcaag aggcagtatt caggcggcgg
     5461 atgcaaatat tcttgccacc tacggcatta atgcctaccg cgtgctggct gatcgtctgg
     5521 gtgtggacca gaaagtggtc agaaaacttg gtgaaaaagg gctgcttggg ccggacagta
     5581 ttcgcctgtt atttcagacg ctggcggaac aggccagggg agcacaaaag gacgcaatga
     5641 attcctggag tggcctgacg gccatgatgg gtgacgtctg ggatcagttt gcccgggatg
     5701 tgatggacag tgggccattt gaaaaactga agggtaatct caggggattt ctttcatggg
     5761 ttgacagtgc caaatctgat ggcagttacc attccctggc ggaaagcacg gcatcagcca
     5821 tgaaccaggg ctttgagtat gcccgtgatg cagtgacagg gttttatcag gcgatcagaa
     5881 aggtacgcga cacgcttcag gctctgcggg atgcgggcta tggtgatgcg ctggatcgca
     5941 ttggtcaggg ggcacagacc gccgcgaaat acctgctgta catgtatctc gccacccgtg
     6001 ccctgaaaat ggcgcgggct gtcggaatgg ggattatacg tccgggtgct gcattgctgg
     6061 gatacggatt atcagcggcg gccttcctga cttcaccttt ccggcgctct cctccggccg
     6121 gaacaccacc aggcacatct cagggacgag ggcaacgttt tataaacttt cttaccgggg
     6181 taaatccggc agccgttcag ccagtacttg taacaaactg gcccacagga ggactgtccg
     6241 gcaccggcgc gaccgatact tcctcaggcg gacgtcaggg gcggggccgc aggaaacgcg
     6301 gaccggggcg cggaagaccg gtcacgcctc ccctgccacc gtccccagtg ccaccgtctc
     6361 cctcttctgg tggggcaggg ttctgggggc gcatgatggg gcgcgccggt gggttacttt
     6421 cgtctgtcgg tagccgcatg gggctggggc gtttctccgg ctttttccgc agtgccggtg
     6481 gactggccgg ccggctgggt ggcggtgccc tgtgggctgg cgcgatggca gccccggttc
     6541 tgctggatgg cagcgccagt gccacagaca agggagaagc ggtcggctct cttgcaggca
     6601 gtattgccgg tggtgcgctg ggggcggcag ccggacctgt ggggattgcc attggttcta
     6661 cggtgggcag ctatctcggt aactatctgg ggggatggct gactgaagcc tggcagaaat
     6721 tacggggcag tgatgatgaa agcagtggac aggcggtaca aaaagcctct gcccgtgtgg
     6781 agctggttgc cccggaaggg tggcaggctc gcagtattga tattgatgac acgtccggtc
     6841 acggactgga tgtgaatgtc tggaacggag ggaattatgg cctctggtga cggtcgtggt
     6901 gcgttccgtg gtgtgccgtt cctcgtctgg cgtgagcagc gcgaacgtgg cgggcgaaac
     6961 attgtccgtc gggaatatcc gttacgggaa accggtgggg cagacgatct gggacctaaa
     7021 ctggctgaat ttacgttcag tgtacttgtg atgggggatg atgttcagac gcagcgaaat
     7081 cgtctgcgtg atgcccttcg tgctcccggt gccggggagc tgcttcaccc ggattacggc
     7141 acgttaaatg tgctgataaa cagctttgaa agtcgttata acgccgctga acagggcgtg
     7201 gttgagttca ccattaatgt cacgccggta agtgatgata ccgcacctgc tgtcacacag
     7261 gacacggccg ccattctgga gcagaagagt accaccgcgc tcgggcaggt ttttgaaacc
     7321 ctggaagcgg gctggacggt gatctctgac ggaatgcacg atgtccaggc aatgaccgaa
     7381 accatcagtg ataaggtgtc cgccctggaa aatgccgttt ccggcatggg gattgtacag
     7441 gatatcagtg cctttactgc cacgttcacg gcgctgaaag ggaatgccac ggcattactg
     7501 acagcacctt cccgcatggc ctcatctttt gcggggcttt tcagtgccct gatcaccctg
     7561 ccatcgctgc cgtcactgtc ttcaggaggc ctgaataccc ggcccggtgg cagtatcggc
     7621 cggacgacat ccgccgtctc tcagggaatg ccacaactgt acaggacatt atcttccctg
     7681 cgttacgtgc tggatgagca ggataatccg cagactctca tcggtctgac accggcagca
     7741 caaaagaata ttcgtctgat acgggcggta atgcagagtg ctgccgtggt ggcccaggca
     7801 cagaccgtgg gaaaactgct ggatcaggtt ctcagtcagg aaaccctgcc ggacagtgac
     7861 gcagcccacc gtacctggcc tgtctggctg gaaagttcag ttgatcttca gcgcattaac
     7921 cgtgacttaa gcgaagcgct cgaacggcag gtgatgacac tgtccgggca ggggtatacc
     7981 gccacggcgc tgacgcttcg tgatgccaga ctggcactga cagaggatct gaatacacgg
     8041 ggagttcagc tacccggtgc aacagtagtg actgtacgta ccaccgagcc tgcactggtg
     8101 accctgtacc gtgccaccgg gaacagtacc ggctggcaac gttttgtgcg ccgtaacggt
     8161 attgttgatc cgctgttcat tcccggaggc cattcagtgg aggtgattag tgagcagcag
     8221 ggttgaactg tatctgggcg gtgagatttt ttccggctgg ctgacggtga gtgttcgtcg
     8281 ctctcttgaa catctggcgg gctcctttga actgggggta atgatgcccg gtgtacgcct
     8341 tccgtcatcc gtccgtgccg gtcagtctct ggaattgcgc attgacggtc agcctgtgat
     8401 cactggctgg ctggatcagg tccggcagcg catcagcgcc acgcgttttc agatcacgct
     8461 cagcggacgg gataaaaccg gtgacctggt ggactgttca gccattcatc cgggcagcca
     8521 gtggaggaac cgcacgctgg agcacattgc ttcagatttg tgtgctccgt tcggggtcac
     8581 ggtgcgctgg caggtaaatg atgcaacggc agcccggccc ttttccacct tcacactgga
     8641 aaattcagaa accgtggcag atgcgctgac acgggccgcg agacacagag gcgtcctggt
     8701 aaccagtaat gccgccggtg aactggtttt cactcaggcc ggcagtcagc gtggcgacac
     8761 gctgacgctg ggcgaaaatc tgctggattt ggatcacaac gtggatcacc gtctgcgcca
     8821 cagtgaatac cgtgtacggg ggcacgggcg tggtggtggt catgccgggg atgcactgac
     8881 agccggaacg ctggccgcac cagttggtac ggtgacagac agtgccatcc accgttacag
     8941 accgaaaatt gtgctggcgg atcatgctgt tgatgcagac ggtgcacgcc agagggctgt
     9001 ccgggaaatg cgccgggcgg ttgcccgctc tgtgcgcctg acagccaccg tgcggcactg
     9061 gtttcgggag aacggccagt tgtgggatat caacctgctg acggctgtca cggctccccg
     9121 caccggagtg gaagagcgtg atcttcttgt ctgtcaggtg gagttttcgc tggatgcaaa
     9181 tcacggcgaa accacccgtc tgattctggc accccgtgac ggctttattg ttccggcaga
     9241 gccgggaaac agcggaagcg gaaatgcggg tgacgtggac gccttcgtgc gggcacagat
     9301 gaaaaaacag gggattaaat tcaatgatga atgacgaagt catcagccgc cttctggccc
     9361 ccgtgatgcg gggtgttcgt ctgctgttcg ggcgtggtgt actgaccggc acaacggaca
     9421 cgctgaaaat ccagaatgtg caaatcaccg gtatggatgg tgaaaccttt gatgacgttg
     9481 aacgccccca gcagtacggg caaatcagcg tccccctgcc tggtgcggaa gtttttctgg
     9541 cctgtgctgg cggacaacgg gatcaggccg tggtgcttgt ggtggaagac cgccgcagtc
     9601 gcccgaccgg acttaccgcc ggagataccg gcgtgtatca ccatgagggg catcgtatac
     9661 gtctgacaaa gaacggccgg atcattgtga cctgtaagac gctggagatt tacgccgatg
     9721 aaggtgttca ggtggataca ccggaggctc actttaccgg taatatcaca gtggataaga
     9781 acctgcatgt caaaggcaat gtgtccattg acggcaccgg aagatcacag ggggcgttca
     9841 cgatgtccga agcggttatt gccgggatca cctattcagg tcatgtgcat cacgataacg
     9901 gtgaaggcag caaaacggga ggaccagaga atggctgata ttgctgtcgt ctgggatcag
     9961 ggttgtggtt ccctgcaact gaacggcgca gatcttctga cggataacag cctgctgact
    10021 gcggtcatta tttcactgtt tacggacagg cgggcgctgg attccgatga aatccctgac
    10081 ggcacccgtg accgtcgggg atggtgggga gacagtttcc gggagcgccc cattggctcc
    10141 cgtctctggc tgttaagccg tgaaaagacg ctgtcctccg tggtcagccg tgcacaggcc
    10201 tatgctgatg aagcgctggc gtggctgcat aaaagcggtg ttgccacatc cgtggtatgt
    10261 catgccatgc gtgtggggca tgatcgcctt tcgctttccg tgaaaatcac cctgccggac
    10321 ggaagcagac atccgatgat tttttatgct gatatgaagg gggaatgatg ccttatcagc
    10381 ccttaccact ggcgcaactg atcacacaga cacaacagga tatcagccag cgcctgcccg
    10441 gttcgcagcc gggcgtgaat gaaaccaccc tgaatgccat tgcgtatgca ctggcgggtc
    10501 tgtcagcaca ggagcatgaa catctggcct ggatctcccg gcagataatt ccgacagaag
    10561 ctgatgaagc cgaactcctg aaacactgcg cattctgggg tgtcatccgt aaaccggctt
    10621 cccgcgctga cggaccggta caactgatgc tgaccacgga tgcagggatc acggaaggcg
    10681 tactccttca gcgaagcgat ggtgttgtgt accgcatcac cggctctgcg accggaaaag
    10741 ccggaacact gaatgttaat gtggaggcgg aaagtgcggg gcgcgccgga aataccccga
    10801 ccggaacccg cctgtccttt atcacgccac aggcgggcat caaccagaca gccacggtta
    10861 ccggcacggg actcaccggt ggtgcggatg tggaaacggt gccggagttg ctgtccaggc
    10921 tggtattccg ggtacagaac ccgccatcag ggggaacaca gtatgatttt gaacgctggg
    10981 cacgggaagt accgggcgtg acgcgggcat ggtgtaagcc tgaatggcct gaggcgggta
    11041 gtgttggtgt gacttttgtt caggataata accctgacat tttccccgga gaaggtgatg
    11101 tgaagcgggt ggcggattat atccgcagtc atgatgatcc ggcgacgggc cagcccgttg
    11161 gtcagccact tgggccgaca atcagcgtgt ttaagctgac caataagccg gtggcgtttg
    11221 agatcaggat tgtacccaaa acgccggaaa atcaggctgc cgtaaaacag gcattaacgg
    11281 acctgcttta taacgaatcg cggccgggtg gacttgtatt gccttcatca ttctggcggg
    11341 ctgttgcagg ggtgaaagga ctggaggatt ttgaagttcg cagtccgctg aagtccgtga
    11401 tggccggaga tacagagttg ctgactgtgg gggaaatcac atggctgtaa ccctgacccc
    11461 gcatcagcgc gccctgttgc agttgctgcc tgacgggctg gcatgggata agcggccgtc
    11521 atccgttctt gcagctttgt gcctgggcct cagtcattcc acggagcgtg tttcctggac
    11581 cggtaaccag atgctggcag aacgttttcc tgattcatcc cgtctgctgc tggaagactg
    11641 ggagcgttat ctggggttac cggaatgtga tatgaccggc gcaaccattc aggaacgtca
    11701 gcgttatgca gggaataaat accggatgaa accctctctt aaccgtgaat tttatatccg
    11761 gtttgcggca gagtttggtt atgaaataga tattcagcca tcaccggatt cacagtgggt
    11821 cagtattgtc acgataaaca gtgaaaccgg ctaccggaat atgaatgtgc tggatgatat
    11881 tctcaccccg ctgcgtattt atgagggtgg tgcgctggaa tgtattctga accgttataa
    11941 gcctgcatgg cagacgttta tttacgtata tgcaaacagt cacgaagagg agactattta
    12001 atgttccatg ttgataataa ttccggcgtg gcgaatatgc ctgaactggc tccggcgcag
    12061 agtaatacca ccacctggtt taccgaaggt gacggacaaa aaggtatcag ctggattggt
    12121 caggactggc tgaatattct ccaggccgaa ctgctgaata ttctggctga agccagtatt
    12181 cagccggata aggcgcagtt aaaccagctt acgctgtcca ttaaagccat tatcgctgcg
    12241 aatgcctttt cccggaaaaa caacctgaaa gaaattgctg atgccggtgc ggaagcccag
    12301 cgtctggccc gtggttatct tggtctgggg acgcttgcca caaaaaacag tcttggtccc
    12361 gctgacgtta atgccctggc gaaggatcag aaccttgccg acctggagaa tgcggaaacc
    12421 gcccgtaata atctggatgt ttacagcagg agcgaaggtg ataaccgtta cctgcgcagg
    12481 gagcagaacg gcgcagacat tccggataaa ggcgctttta tcgataacgt cggtttacgg
    12541 gaaacggtaa ataaggctgc tgatgccctg ccatcgggtg gaaccgccgt ggcagcaaac
    12601 agactcgcca ccccaagaaa tattaatggc gttccttttg acggaacgca ggatatcaac
    12661 atcacgtcag gaatgacgca gtcaaccgca gatggccggt atgtacagaa tgttcagctt
    12721 ggagcacaga gctatcattc acccggaggc aatgaaatat catggaatta cagcgcacct
    12781 tccggttgta tgctttccgg tattaacgtg caggaaaccg gctcccggtc tgcggacaat
    12841 atcggtgggg tctattatcg cccggttcag atttatatta ataatgcctg gagaacggta
    12901 agttcagtgt aaaccacaga aaagggtgct gaatgcaccc tctgaattat tccggttttt
    12961 ccggccaggg aatcgcctgg taatgcccct cactggcaat atcattaaag tgcatttctt
    13021 taagctgtcg gatatacgcc atccagcgcg tcagctgtaa tttattttca tcgctaatca
    13081 tgtccagttt taattctgtt ttccagtcat ccacggtttc gttggcttcc tggagcagcg
    13141 atgagcgacg cttttcagca accgtcttcc agtttaccgg cacctttgat atcaggccat
    13201 cagaaaactg ccagttaccg tcaatattca caccttccgg cagttcgtcc acttcaacaa
    13261 cagtaaaacc tgccggatat aaagcagaga catcttcagc aacagagcag ataatacccg
    13321 ttacaggcga aatacagagt ttgtatttct ttgtgaaaag cgggatagat tcataaaaat
    13381 cccttccgtc ctcactctgg aaatactgaa catcctcacc atagggtttg tctttcgggt
    13441 aatatcgttt gacattaatc agttgcataa acatcaccat gtaaatcaat gataaaaaca
    13501 ggaataatac tatcgccata tacaggcaat gcttgtctga aataacgcat atttcagatg
    13561 ctggctgctg tgtgctatgc ccccgttaat taaatacttg cagcggatac ccaatgccca
    13621 ttaatacaat attgtatctg tctgtaataa acacggtcat caccaccacg catttcccag
    13681 tctccgttag tcttaaaacc ggtaatcaca tttcctgatg acagggagta attatattcc
    13741 agtccgccgc ttaatgcctg cgctcccagt cttacccctg taacataacg ctgattggcc
    13801 gttgattccg gcattcctga cgtaatgttt atatcctgcg tgccgtcaaa ggcgacacca
    13861 ttgattctgc gggcatttgc cagcctgctt gctgcaacag ccgtaccgtt tgatggcagg
    13921 gcattcgcgg ccctgtttac cgtatcccgt aaaccgaggt ttcagggatg ccgtatatct
    13981 gctttaaaag gcctttccac ctctttttaa ggaaggtaaa tacatgctga ttggctatat
    14041 acgcgtatca acaaatgacc agaacacagg tttacaaaga aatgcgctgg aatgtgcagg
    14101 atgtgagcag atttttgaag acaaaatgag tggtaaaacg acacagcggc cgggactgaa
    14161 gcgggcactg aagaaactaa aacccggtga cacgctgatg gtctggaaac tggacaggct
    14221 ggggcgcagt atgcgccacc ttgttgcgct gacagatgaa ttacagcaac aggggataaa
    14281 ttttcgtagt tttactgaca acattgatac gtcaaccccc atggggcgct tcttttttca
    14341 cataatgggt gcgctggcag aaatggagcg tgaattaatt gtcgaacgga cacgggctgg
    14401 tcttgcagct gcccgtgaaa ggggacgaac cggcggcaga ccccgccgtc tcacgcagga
    14461 acaatacgag caggt
//
LOCUS       DAZUFB010000121        14287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZUFB010000121 DAZUFB010000000
VERSION     DAZUFB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.121"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..918
                     /locus_tag="ACHY3M_004118"
     CDS             <1..918
                     /locus_tag="ACHY3M_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318933.1"
                     /translation="SGGNLIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAAST
                     NVYPITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDV
                     VINIPQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKEN
                     ESAETVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLI
                     QALFFNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLF
                     HAPTIFYQYPKLIDEVKYCISNKKITVRL"
     gene            957..1784
                     /locus_tag="ACHY3M_004119"
     CDS             957..1784
                     /locus_tag="ACHY3M_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318934.1"
                     /translation="MSPLLMLNGAVKTGSIVRTIGRGVSYVDIPLALAILGDSWYKAY
                     KHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSLEKGTPPQMMSLF
                     IVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVDLQHTKNITMPLF
                     KTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGERAILNTSQSETCKHKIDEIYEKR
                     FSETELNTLPNEMKNFLIFLNKTHEKDISRLSMGNEVNEKIIAVIVKTLAYRESMLC"
     gene            complement(2288..2470)
                     /locus_tag="ACHY3M_004120"
     CDS             complement(2288..2470)
                     /locus_tag="ACHY3M_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS4318935.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            2627..2971
                     /locus_tag="ACHY3M_004121"
     CDS             2627..2971
                     /locus_tag="ACHY3M_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318936.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            3077..3295
                     /locus_tag="ACHY3M_004122"
     CDS             3077..3295
                     /locus_tag="ACHY3M_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4318937.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            3273..4343
                     /locus_tag="ACHY3M_004123"
     CDS             3273..4343
                     /locus_tag="ACHY3M_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4318938.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIS"
     gene            complement(4338..4964)
                     /locus_tag="ACHY3M_004124"
     CDS             complement(4338..4964)
                     /locus_tag="ACHY3M_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFS4318939.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKNFINYQYVNVRWSLLIKIKPL"
     gene            complement(4961..6649)
                     /gene="yfaA"
                     /locus_tag="ACHY3M_004125"
     CDS             complement(4961..6649)
                     /gene="yfaA"
                     /locus_tag="ACHY3M_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFS4318940.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(6798..9425)
                     /gene="gyrA"
                     /locus_tag="ACHY3M_004126"
     CDS             complement(6798..9425)
                     /gene="gyrA"
                     /locus_tag="ACHY3M_004126"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFS4318941.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            9572..10294
                     /gene="ubiG"
                     /locus_tag="ACHY3M_004127"
     CDS             9572..10294
                     /gene="ubiG"
                     /locus_tag="ACHY3M_004127"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFS4318942.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(10422..14156)
                     /gene="yfaL"
                     /locus_tag="ACHY3M_004128"
     CDS             complement(10422..14156)
                     /gene="yfaL"
                     /locus_tag="ACHY3M_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFS4318943.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAASGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNANVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         3758 a         3677 c         3253 g         3599 t
ORIGIN      
        1 tcaggaggaa acttgattcc aaattcaact tcaaataaag gttatgtatg cattgacatg
       61 cagtgttcgt caacctctga accaacagct tctacctcca gcaaccggag tattaaatta
      121 gccgcttcca caaatgtata tccgataaca agaaacgact ccgagctcac tctgaacgat
      181 tttcttgata atagctcttc tacgtcatca ttggactaca ttaatgaatt gggttctcaa
      241 ctgacgttaa atgattttct tgacaacata aagacaaatg aggtggatag aacatgtacg
      301 gatgtggtaa ttaatatccc acaagagata caaacaaata cacaggaaaa tgatttgtta
      361 ttatccgata aaaataattc aatatgcatt gaaatcgatg aaagaattac aaaaatccta
      421 acatgcaagc aaaaatatca actggacagc atcattcatg aaattatacc aaaagagaat
      481 gagagtgcag aaactgttct ccatcttatg agagttctga acgatcaata tcatcaggta
      541 tataatcagt caggatgttt ttataaagcc tatatggcca tacacaataa aatcgaacag
      601 atacttccat atgcgttcag agccggaggc ggaatcagca ttcacttgct catacaggca
      661 ttatttttta atggcgacta taacaaatca ccttcacagt ctcaacaacc atctttatat
      721 acatcacctt ctccaacaat aaatacagaa gcattcttaa gtaatgtatt atcactagat
      781 ataacccagg tacgcatact tggtgattta ctatcagcaa ctttatttca tgcaccaaca
      841 atattctatc aatatcctaa actaatagat gaagttaagt attgtataag taataaaaaa
      901 ataacagttc ggttatagca cgatttactc tatgtttaac aagtacatta ctaaccatgt
      961 caccactgtt aatgcttaat ggagcagtta aaacaggtag catagtaaga actataggta
     1021 ggggagtgag ttatgttgat ataccattgg ccttagctat attaggtgac tcgtggtata
     1081 aagcttataa acatggttct tctgataacc caaattctgc tcagagattt atatcgcaag
     1141 aagcggcctt taaaaccacc cagcgggtat taacacaagg attaagtctg atgtcctctt
     1201 tatcgggagc aatcatgcgc tctcttgaga aaggcacacc accacaaatg atgtctttat
     1261 tcatcgtaaa catactaaat ctattatttc atcaaaatcc atatgaaggg gcatcagcaa
     1321 gtgctaatgc tttgaaaaga tcaacttact cccataatcc ggacatacta aatactcagg
     1381 caatagctct ttgtgttgac ctccagcata caaaaaatat aaccatgcca cttttcaaaa
     1441 caaaagacag gatatcttac gcattcaatg gacaaagaac atccccagaa gaccaaaaac
     1501 aaatactgaa agaagttata aactcctgta cccaaggaga aagagccatt ttaaatacat
     1561 cacaatcaga aacatgcaaa cataaaatcg atgagattta tgaaaaaaga ttctcagaaa
     1621 cagaactaaa tacattacca aacgaaatga aaaatttctt gatattttta aacaaaactc
     1681 atgaaaaaga tatttcgcgt ttaagcatgg gtaacgaagt taatgaaaaa ataattgcag
     1741 ttatagtcaa gacgttagca tatagagagt ctatgttgtg ttagacttta actctatatt
     1801 gatataacat tagccaaata ttcaacagtg tatgcgacca aacaccaaca tgtcgcatac
     1861 atatacaatt tgaatattta attatattta cttaatgtat ttctataaaa gcaaattaca
     1921 aactcacaac aaaaaacctc ataacacatt aacaatcaat tcttttcatc tttataaatt
     1981 ctcatatcag gcttgcaccc gataaaccga cgaaaactat ttaaaaccca tcgagtgaag
     2041 taatctctaa aaccaaagaa ataccaagtg aaaatattca cgataaaatg cccggtcaaa
     2101 gcccctcctg taccgcatgc aagaacagta aaaaatcaga tgttttcata aatatcagtc
     2161 ctcatcgttt tgcctggcat gtcctttacc agcaatcttc tgtatgcact aagcctagat
     2221 agaatccact cagtgtacac tgaagcccgc tcgacgcttt cttgttcgta acttcgattt
     2281 tagtcaatta ccttgttttc ctcgcacgat gtcttagcca ccggatatcc cacaggtgag
     2341 ccgtgtaatt gaaggttttt acgtcagatt cttttgggat tggcttgcgt ttatttctgt
     2401 agcgtttcgt tggaaggtat ttgcagtttt cgcagatgat gtcggtgaaa cttcgtcgct
     2461 gtcgcctcat gccgccctcc tgacgccctg cccgatcgcc atcaatgccg ctttggatca
     2521 gttcgcgata aaggtgaata tcggcaatga ataacaatcc tcgcactcgc ggggatttct
     2581 tttatctgaa ctcgctacgg cgggttttgt tttatggaga caagaaatgt cagatttggc
     2641 tatgaaggtt ttgaaatggc aatcgactgg cgatgttggc atcagtagcg caactcttgc
     2701 ctcaatcgca tgtggactga aaaagaatat ctatggtcat cacttcggcg ctccacatga
     2761 cgcagcagac ttccggcgat gcgttgcact tgttgagcag attccagaaa tcagagattc
     2821 attcgacaag gttgcaaagc gcgttccggc attcaaaggc atcctcaacg aatgggattc
     2881 cctcgtcgct ctgttgaagt ctgaaatgaa gatacacgga aacaaagcac cagagactta
     2941 cagaagaatt agcgagctac gcaaggacta accacagcct cacactcaat gaggcctgtt
     3001 catttctcaa gatatccaga cctaccattg ccgcatcaat gcggcttttc ttgcgtgtaa
     3061 ttgcggagac tttgcgatgt acttgacact tcaggagtgg aatgcacgcc agcgacgccc
     3121 aagaagcctt gaaacagttc gtcgatgggt gcgcgaatgc aggatattcc ctcctccggt
     3181 taaggatgga agagagtatc tgttccacga atcagcggta aaggttgact taaatcgacc
     3241 agtaacaagt agccttttga agaggatcag aaatgggaag aaggcgaagt catgagcgcc
     3301 gggatttacc ccctaacctt tatataagaa acaatggata ttactgctac agggacccaa
     3361 ggacgggtaa agagttcgga ttaggccgag acaggaggat agcaatcact gaagcaatac
     3421 aggccaatat tgagttgcta tccgggaaca ggcgtgagtc actgatagac agaattaaag
     3481 gcgctgacgc aatcactctt catgcgtggc ttgaccgata tgaaacaatc ctcagcgaga
     3541 ggggtatcag gccgaaaact ctactcgact acgccagcaa aatcagggca atccgaagaa
     3601 aattgccgga caaaccgctc gctgacatat caacgaaaga ggtggcagca atgctaaaca
     3661 cctacgtagc agaaggtaaa gcggcttccg caaaattaat caggtcaacc cttgttgacg
     3721 tttttcgtga ggcaatagcc gaggggcatg tggctacgaa tccggtaaca gcaacccgca
     3781 cagcaaaatc agaagtaagg cgttcaagac tgacagctaa tgaatatgtc gcgatttacc
     3841 atgcagccga accgctccca atctggctga ggctggcaat ggatttggct gtcgttacag
     3901 ggcagagagt aggcgattta tgcagaatga aatggtcaga cataaacgac aaccatcttc
     3961 acattgagca gggtaaaaca ggggctaagc tcgccattcc gctgacgcta acgattgacg
     4021 cgctcaatat ctcattggct gatacactac agaaatgcag ggaggccagc ggcagtgaaa
     4081 caataattgc atcaacgcat cacgaaccgc tttccccgaa aacagtatct aagtatttta
     4141 caaaggcgag aaatgcatct ggactctcat ttgatggaga cccgccaaca ttccatgaac
     4201 tgcgcagcct gtcggcgagg ctataccgga atcagattgg cgataagttt gctcaacgtc
     4261 ttctcgggca taaatcagat tcaatggcgg cgcgttacag ggacagtcgc gggcgggagt
     4321 gggacaaaat tgaaatatca taatggtttt attttgatta ataatgacca gcgcacatta
     4381 acatattgat aattaatgaa atttttaata tacaacttcc atgtcataag ttgttgcaga
     4441 ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa
     4501 cgccccatcc agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca
     4561 ggcagcgcct ggtttatgtc ctggccaata aactggctgt tgtactgaat caaattaatc
     4621 gcggtcacgt aggggccggt tttcccgttc ccctgattcc agttttgcgc cagttgacgc
     4681 tgtccaggtg ttagcgtcat ctctggcggc aaatactggc tagagaaacc gttacttttt
     4741 agccatttac tatcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca
     4801 caatcctgct gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt
     4861 acaaaccagg cgcgaaacag cccggattgt tcgacattca gcatttcgct atgggcaaca
     4921 acacaacaca gccagcaaat cagcgccagc agcccgtgcc tcatagcggc tgccaggtta
     4981 tgggtagcca ctgccaggcg gcaccgggtt ccatctgggc cagcttcatg acataacgcg
     5041 gttgttgaga taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa
     5101 ccggttcgag attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc
     5161 cttgtggatt gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg
     5221 ggcggttttt attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg agggagaaaa
     5281 gcagcgtttt gttttgcatc gccagcgaca cgcggaaaaa ataatccgac attaattgat
     5341 cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacgcc
     5401 agatttgcgc ttcgccctgc tgagtctggc ttatcggcaa aacaccttct ggcgctttgc
     5461 tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag
     5521 tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt tgagtctgca taccagcaca
     5581 gccccgcggc accgtctaac gccccattca acttgtcgtt ttcctgactg atgtgcgaaa
     5641 gcatctcttc ggcaataccg tgtgaatacg gcaccaccac acagaagctg gccccggcag
     5701 gcatactgtt ccagaccggc gtaaaatcga aactggtatc tacgctggcg gattcatcat
     5761 ttaacgccac aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag
     5821 aaggcattaa ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgctggc
     5881 gtactggcgt tttttcagca gcacgctctt ccaggccaaa gcttgcttgc cagcgttttt
     5941 tgccgctcaa caaatcacct gcgatcgccg tggcttcggt atcctgctga tcatctttaa
     6001 acaacatatc cgtgctggaa aacaccagca tcttgtcctg ataagtcgcg aacatcaggg
     6061 cgttattgcc gttatagcgc aactgataaa caggaacagt ttcactattt atcttgatgc
     6121 tactgatttc cgttttgctt aactggctat cgctggtagc ggcaaacaac aatggctcca
     6181 gcagtttgct taaaccactg cgctggatca gcaccatata atgtgaaaga tggccttgtt
     6241 tatcgtgcca cagcgctgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt
     6301 tcagcgtgag atcgtgttca tagacaatac gacgaatgct gccttcaatg cccagacgat
     6361 cggcatgatt ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa
     6421 tggtgaggag atctttggga agctggctca gagagtcgct gtcgagaaag aggtccggct
     6481 cattgagatc gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc
     6541 cactgccagc atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac
     6601 ctacaagacc atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc
     6661 ttgtgtatag ccagccattt tttacgggca cagccaaact ttaccgtgcc ctaatacgac
     6721 aaaagcccag actttgcagc ctggactttt caattcaaac aagggagata gctccctttt
     6781 ggcatgaaga agtaaaatta ttcttcttct ggctcgtcgt caacgtccac ttccggagcg
     6841 atttcatcgt ccccttccgc ggcactgcca tcgatggtat ccagatcttc ctcgtcaacc
     6901 ggttcagcaa cacgttgcag acccactacg ttttcatctt ccgcagtacg gatgaggatc
     6961 acgccctggg tgttacggcc tacgatgctg atttccgaaa cgcgagtacg taccagcgta
     7021 ccggcatcgg tgatcatcat gatctggtcg cagtcatcta cctgtaccgc gccaaccact
     7081 aaaccattac gttcagtaac cttaatggag ataacccctt tcgtcgcacg cgacttggtt
     7141 gggtattccg ccactgcggt acgtttaccg taaccgtttt gcgttgcggt gaggattgcg
     7201 ccatcgccac gaggcacgat cagagagacg actttatcgc cttcacctaa gcgaataccg
     7261 cgaacaccgg tggtgttgca gcccatcgca cggacagaag actctttaaa gcgcaccact
     7321 ttaccttcag cggagaacag cattacttcg tcttcgccgc tggtcaggtc aacgccgatc
     7381 agctcatcgc cgtcaaccag tttgatcgcc actttaccgg cggtacgcag acggttgaac
     7441 tcggtgagga cagttttctt cacggtaccg ttagcggtcg ccatgaagac tttcacgcct
     7501 tcttcaaact cggtcactgg caggatcgca gtgatacgtt cgtcctgctc cagcggcagc
     7561 aggttgacga tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg ataaactttc
     7621 atcgaataga cgcgaccacg gctggagaag cacagaatat ggtcgtgagt gttcgccacc
     7681 agcagtcggt cgataaagtc ttcttcttta atacgtgcgg cagatttacc tttcccgcca
     7741 cgacgctgcg cttcgtattc agaaagcggc tgatacttaa cgtagccctg gtgagagagc
     7801 gtcactacca catcttcctg agtgatcaga tcttccaggt tgatgtctgc gctgttggcg
     7861 gtgatttcag tacgacgttt gtcaccgaac tgttcacgaa ccagctccag ctcttcacgg
     7921 atcacttcca tcaaacgatc ggcgctacca agaatacgca acagttccgc gatctgatcc
     7981 agcagctctt tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag tttctgcaaa
     8041 cgcagatcca gaatcgcctg agcttgctgt tcggtcaggt agtacagacc atcacgcacg
     8101 ccgaactctg gctccagcca ttccggacgc gcagcatcgt cgccagcacg ttcgagcatc
     8161 gcggcaacgt tgcccagctg ccacggatta gcaaccagcg cagttttcgc ttctgcaggc
     8221 gtcggcgcat gacggatcag ttcgatgatc gggtcgatgt tcgccagcgc cacggctaat
     8281 gcttcaagga tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt acgacgggtc
     8341 accacttcac ggcggtgacg aacaaacgcc gcgatgatgt ctttcaggtt catgatcttc
     8401 ggctgaccat ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa ctgggtctgg
     8461 gagtagaggt tgttgagcac aacttcaccg accgcatcgc gtttcacttc aatcacgatg
     8521 cgcataccgt ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac gcgtttttct
     8581 tttaccagtt ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata cggaatttcg
     8641 tggacgataa tggtttcacg accggttttg gcgtcaactt ccacttctgc gcgagcgcgg
     8701 atatacacct tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg accgttaatg
     8761 attgccgccg tcgggaagtc cggccccggg atgtgttcca tcagcccttc aatgctgatg
     8821 tcttcatcat cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt gtgcggcggg
     8881 atgttggttg ccatacctac ggcgataccg gaagaaccgt tcaccagcag gttaggaatt
     8941 ttggttggca tgacgtcggg aattttttcc gtgccgtcat agttatcaac gaaatcgacc
     9001 gtctcttttt cgagatcggc catcagttca tgggcaattt tcgccagacg gatttccgta
     9061 taacgcattg ccgccgcaga gtcgccgtcg atggaaccga agttaccctg accgtctacc
     9121 agcatgtaac gcagcgagaa tggctgcgcc atacggacga tcgtgtcata aaccgccgag
     9181 tcaccatggg gatggtattt accgattacg tcaccaacga cacgggcaga ttttttatag
     9241 gctttgttcc agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg gtgtaccggc
     9301 ttcaggccat ctcggacatc tggcagcgca cggccaacaa tgaccgacat cgcataatcc
     9361 agataggagc tcttcagctc ttcctcaatg ttgaccggtg taatttctct cgcaaggtcg
     9421 ctcatctaac cgctatccct ctactgtatc ccggattcaa aggtcgcaaa ttataacaca
     9481 gccgcgcagt ttgaggtaaa cctatacgct ttattcacat ccaatgcctg atatactcgt
     9541 ttgtcttgcc aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc cggaaaacca
     9601 taacgtagac cacgaagaga tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct
     9661 tgaaggtgag ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct atattgccga
     9721 gcgtgctggc ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct
     9781 ggccgagagt atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg gctttgagcc
     9841 attgcaggtg gcaaaactgc acgcgctgga aagcggcatt caggtggatt atgtgcagga
     9901 aaccgtggaa gagcacgcgg caaaacatgc cgggcagtat gatgtggtga cctgcatgga
     9961 gatgctggag cacgtccccg atccgcagtc agtggtcaga gcctgtgcgc aactggtgaa
    10021 accaggcggc gatgtctttt tctcgacgct taaccgcaac ggcaagtcat ggctgatggc
    10081 ggtggttggc gcggaatata ttttgcgcat ggtgcccaaa ggcacgcatg atgtgaagaa
    10141 gtttattaaa ccggcagaac tgcttggctg ggtagatcag acaagcctta aagagcgaca
    10201 tataaccggg ctacattaca acccgctcac taatactttt aaactcggcc ccggcgtgga
    10261 tgtgaactat atgctgcaca cgcagaataa gtgaggttgt tgtttggccg agccaatgcc
    10321 tgatgcgacg cttgccgcgt cttatcaggc ctacaaatgc tccccgtagg ccggataagg
    10381 cgtttacgcc gcatccggca accgtgccga ctagacagtg attaccattt caccgtcatc
    10441 gacaaaaacc ctgccgtctg ggcaaaatca tcactccctt tctgccacgc cacgctgccg
    10501 cgcagggaca ctcgctgact gatattgccc gtgactccca cttttatttc accccgtcgc
    10561 ttcaccgcat cgtcactgat agtgctgcca tcttcttcaa tttccgtcgc atggggatcg
    10621 tgataataat tcagatctaa tgttggtgtg acaccaaccg cggtacgcca ttcgctgtgt
    10681 aaacccagcc gcgtctgaat atcatccccc tgcgattgtg acacgcgcgc atggttagcg
    10741 gcggtaaaat catcctgctg cacgccctga taaatcacct gcgcctgcgg ttcaatcacc
    10801 acaccacgcc ccggtaacca ctgatacccg gcttccagcg aggcgataat ccccgacgag
    10861 tggtaatgat ctgtgccatc gtcttgttcg gaaacatcat tgttaaacca cgcgtattgc
    10921 agccagctat ccagccaggc cccctgcttc tgattaccgt gctgatacca gcttgaggtc
    10981 agcccaacgg cataaccgtg gttctggtta tcggcgcgag ttccggtcat attcgagcgg
    11041 ctctcgccct gattatcact gtagccacca accgccccaa gcatccactc gccatcatcg
    11101 ccccagcgcc cgctaaacag gtcaccgcta agctgcaccg tagaagtgtc ttcatgctga
    11161 gccagttgcc ctgccgccgt gtaatgataa cgtccgccga taacacgtaa attcagcgtc
    11221 tgaccattgc cacctgcatg atcgcgtcgc tccatcataa acgcctgatt tgccgcccgc
    11281 agattattga gataaccgcc aacgttggca ttcagcaccg gctggtaagc ggggattgga
    11341 tcgggtgttg ggtcaggatc gggtgttggg tcagggtctg gatcaggtgg caatggtggc
    11401 gttacttctt gcgatcgcag ataccagtcg ttgttatctt ccaccagcgt gtagtcatat
    11461 gctcccatat tgacgtagcc gctgcctgcc agactgaact tcgcattgtt ttgaaactgc
    11521 gtgggatcgg ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct
    11581 gtaatggagt taaccaccac agttgtgttg ccagcagtat taccgttcaa caccaattga
    11641 tcgcttaccg agtcatcgcc gtttaattcg ctatcgagca gtagcgtacc gccgccggta
    11701 taatcaccgt ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg
    11761 gacgtattgg tcagattgcc attaaccgtc acgtcggaaa gataggtcag gctgtcagtt
    11821 tcaaggttgg tattaagctc acctgtcaac gtcacgccat cggtggcatt caccagcgtc
    11881 ccatcgccgt taaacagtga ggcattaagg gcgacagaag tatcctgccc ggtcaggcgc
    11941 aaaaccgtac catcgctgat gtcgatagtg ccgctggaag tagcatcaat agattgaata
    12001 tcctgatccg cgccagtaac gaacgtcgcc ccatcaccga cccacagtga cgaagcataa
    12061 ggaaggatat ccgcgacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc
    12121 gttgtaccgc tggcggtcag ctccagcgta cccgcccccg ttttagtcaa tgtgctaccc
    12181 tcatcctgat gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc
    12241 gcatcaactg ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc
    12301 gtagagcgag tatcgacatt ggcagtaatc tccatcacgc tttcttgttg cttatcggta
    12361 aagataacct ggcggttata gtgggtatct ccaagctgtg agttgtcgct cacctccagc
    12421 ataccggagg cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc
    12481 ccgtcgcccg catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcata
    12541 tcgtttccac ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgccg
    12601 ctgacttcaa ggccatcgtt gtatgatgtg ccactctgcc aggtggagaa atcagagaga
    12661 tcgagcacac cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct
    12721 gccgcgtcgc cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc
    12781 gccatcgact gcgcccccga cagcacgtag ctgccgtttt gcgcaatggt gagctgacca
    12841 gcaccttcga tggtgccagc aaaactgcct tgattaacag taacattgcc accagcatcg
    12901 atatttaaag tgccattctg aaagcccgtc aatgagtgca caaaggtttg ttgggtggag
    12961 ccaacgttta gctctgcctg attctggtac ttatcaatac tccctatcgt cagaccgtag
    13021 cagtcttgcg gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga
    13081 cccagggtaa cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca
    13141 ttaagtacca gatcgccgga acctgttttg gtgatcaacc cggtaccagc aatagagtca
    13201 acagctccgt cattctctgt attgccaata accagcgttt ttccgtcggc aatatcaaag
    13261 gtaacttcac ttaagccgag atacataaag ccactcgccg cagtggaagg accatctcca
    13321 tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct gtagctgtca
    13381 tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca
    13441 taaccttcgg cagtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga
    13501 tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa
    13561 cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata aacgtcatta
    13621 ttaatggtat aaattgcgcc acctttgcca tcattagcga tgttattgcg aaacatggcg
    13681 ttagtgacac gtaaatcgac gacaccagta tcattagtac cagaagaata gattgcgcca
    13741 ccatagccgc ctgcgacgtt accagaaaaa ataacatcag taagattcag cgttgagttt
    13801 tctttagcaa atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac
    13861 agagtcattc cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat
    13921 acggctcctc cgctggcgtt attggtcata tccgaaaaaa ccagccattg cccatcggcg
    13981 acactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
    14041 atatcatatc cctggcataa atcaatggcc gccgcgaaac cgttagcaga gaaaagagat
    14101 gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat ccgcatattc
    14161 atctaaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
    14221 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaatag
    14281 tatcagt
//
LOCUS       DAZUFB010000122        14156 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZUFB010000122 DAZUFB010000000
VERSION     DAZUFB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.122"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..556
                     /locus_tag="ACHY3M_004129"
     CDS             <1..556
                     /locus_tag="ACHY3M_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFS4318944.1"
                     /translation="DNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFF
                     IQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIF
                     ILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKS
                     HGKYALFPIFTASWRATRIKNIGI"
     gene            779..1276
                     /gene="ubiC"
                     /locus_tag="ACHY3M_004130"
     CDS             779..1276
                     /gene="ubiC"
                     /locus_tag="ACHY3M_004130"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFS4318945.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHY3M_004131"
     CDS             1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHY3M_004131"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFS4318946.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHY3M_004132"
     CDS             complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHY3M_004132"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFS4318947.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHY3M_004133"
     CDS             4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHY3M_004133"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4318948.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5388..5996
                     /gene="lexA"
                     /locus_tag="ACHY3M_004134"
     CDS             5388..5996
                     /gene="lexA"
                     /locus_tag="ACHY3M_004134"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4318949.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6069..7394
                     /gene="dinF"
                     /locus_tag="ACHY3M_004135"
     CDS             6069..7394
                     /gene="dinF"
                     /locus_tag="ACHY3M_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFS4318950.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7510..7719
                     /gene="yjbJ"
                     /locus_tag="ACHY3M_004136"
     CDS             7510..7719
                     /gene="yjbJ"
                     /locus_tag="ACHY3M_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4318951.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHY3M_004137"
     CDS             complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHY3M_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFS4318952.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8594..8843
                     /gene="yjbL"
                     /locus_tag="ACHY3M_004138"
                     /pseudo
     CDS             8594..8843
                     /gene="yjbL"
                     /locus_tag="ACHY3M_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
     gene            8867..9574
                     /gene="yjbM"
                     /locus_tag="ACHY3M_004139"
     CDS             8867..9574
                     /gene="yjbM"
                     /locus_tag="ACHY3M_004139"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFS4318953.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9937..10041
                     /locus_tag="ACHY3M_004140"
                     /pseudo
     CDS             9937..10041
                     /locus_tag="ACHY3M_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            10188..10716
                     /locus_tag="ACHY3M_004141"
                     /pseudo
     CDS             10188..10716
                     /locus_tag="ACHY3M_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10913..11086
                     /locus_tag="ACHY3M_004142"
     CDS             10913..11086
                     /locus_tag="ACHY3M_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318954.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(11134..11415)
                     /locus_tag="ACHY3M_004143"
     CDS             complement(11134..11415)
                     /locus_tag="ACHY3M_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFS4318955.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(11452..12024)
                     /locus_tag="ACHY3M_004144"
     CDS             complement(11452..12024)
                     /locus_tag="ACHY3M_004144"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4318956.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYAVVSL
                     DSAMKQGATPDGDTILWWLKQSPEARAAICIDDTLSISDALSELNHFINRHADNTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            complement(12024..12470)
                     /locus_tag="ACHY3M_004145"
     CDS             complement(12024..12470)
                     /locus_tag="ACHY3M_004145"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4318957.1"
                     /translation="MLQGSQPVSQTYNLPELIEGMEVSIDISTCDADLGNRYFGTVTE
                     ALELDTAKNGYILLVQDAEPNFDVNGNSPGTPDSWISCSDRMPEKGQNVLISVNFDSS
                     LVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVK"
     gene            complement(12462..12758)
                     /locus_tag="ACHY3M_004146"
                     /pseudo
     CDS             complement(12462..12758)
                     /locus_tag="ACHY3M_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(12761..12952)
                     /locus_tag="ACHY3M_004147"
     CDS             complement(12761..12952)
                     /locus_tag="ACHY3M_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318958.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            complement(13005..13421)
                     /locus_tag="ACHY3M_004148"
     CDS             complement(13005..13421)
                     /locus_tag="ACHY3M_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318959.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEAEPVTWRYRYVRKGVTNFQEKPWVGGWKYVPTKEDCNDRPNYEIQALFTAQ
                     PVPLTPEGLIKAVRFYEQVKSENPPVETGAWKDAVD"
     gene            complement(13568..14041)
                     /locus_tag="ACHY3M_004149"
     CDS             complement(13568..14041)
                     /locus_tag="ACHY3M_004149"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4318960.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFSMVIFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLRPSGVLIFKWNETQIPVRQILVLTDRKPVIGQRTGKNDKTHWIIFMK"
     gene            complement(14038..>14156)
                     /locus_tag="ACHY3M_004150"
     CDS             complement(14038..>14156)
                     /locus_tag="ACHY3M_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="eae protein"
                     /protein_id="HFS4318961.1"
                     /translation="SAKEELANESHPIDGLSGEYAEKSLEEWAERLRKGGSQ"
BASE COUNT         3480 a         3246 c         3508 g         3922 t
ORIGIN      
        1 agataacaag ttcgatgatt ttatgcgtca acatcgattc agcaaggtgg atttcgatac
       61 ccaacagatg atccatttta ttaacaggtt taatatgaat aaagggctga ttgataaaaa
      121 taacaatttt tttatccaac ttatcgatca ggccttacga tcaacggatg atatgatcaa
      181 agcaaatgcc tggtatcttt ataaagagtg gattcgtagt gatgatgttt cacctctatt
      241 tatagaaatt gaagataatt taagaacctt taacacgaat gaattaacac gaaaggataa
      301 tatctttatc ctgttctcct ctgccgatga tgggccagtt atggtggtaa gctcccagcg
      361 cttacatgat atgttgaatc ctacaaaaga taccaattgg aattccacgt gtatctataa
      421 atccagacat aagatgttgc ctattaatct tactcaggaa acacttttta gctccaaatc
      481 tcatggtaaa tatgcgcttt tcccaatttt tactgcgagt tggcgagcta ctcgtataaa
      541 gaatataggt atttaagtaa aggaaaacat caccgttcct ggcatcatgg gcggtgatgc
      601 ccctacggtt gccctcgcca gcacgggcat cggcaaagcg taaggttcaa cagcggttta
      661 ccacttcatg cgattgttgc gtttttgttg cgcattagat cacttaattt gctttacatc
      721 tcccgtaaac acttttctgc gatacaatgc ctttacgtta tgtaacggag agttcggcat
      781 gtcacacccc gcgttaacgc aactgcgtgc gctgcgctat tttaaagaga tccctgccct
      841 ggatccgcaa ctgctcgact ggctgttgct ggaggattcc atgacaaaac gttttgaaca
      901 gcagggaaaa acggtaagcg tgacgatgat ccgcgaaggg tttgtcgagc agaatgaaat
      961 ccccgaagaa ctgccgctgc tgccgaaaga gtctcgttac tggttacgtg aaattttgtt
     1021 atgtgccgat ggtgaaccgt ggcttgccgg tcgtaccgtc gttcctgtgt caacgttaag
     1081 cgggccggag ctggcgttac aaaaattggg taaaacgccg ttaggacgct atctgttcac
     1141 atcatcgaca ttaacccggg actttattga gataggccgt gatgccggac tgtgggggcg
     1201 acgttcccgc ctgcgattaa gcggtaaacc gctgttgcta acagaactgt ttttaccggc
     1261 gtcaccgttg tactaagagg aaaaaaatat ggagtggagt ctgacgcaga ataagctgct
     1321 ggcgtttcat cgcttaatgc gtacggataa gccaattggc gcgttactgc tgctctggcc
     1381 aacattatgg gcgttgtggg tggcgacacc gggcgttcct cagctctgga tcctggcggt
     1441 atttgtcgcg ggtgtctggc tgatgcgcgc tgctggatgt gtggtgaatg attatgctga
     1501 ccgaaagttt gatggtcatg ttaagcgcac ggcgaaccga ccacttccca gcggcgcggt
     1561 aacagagaaa gaggcgcgcg cactgtttgt agtgctggta ctgatttcgt ttttactggt
     1621 actgacgctg aatacgatga ccattctgtt gtcgattgcc gctctggcgt tggcgtgggt
     1681 gtacccgttt atgaagcggt atacccatct cccgcaagtg gtgctgggcg cggcgtttgg
     1741 ctggtcgatt ccaatggctt ttgccgctgt gagtgagtcg gtgccattga gttgctggtt
     1801 aatgttcctc gccaatattc tctgggcggt ggcttacgac acgcagtatg cgatggttga
     1861 ccgcgatgat gatgtgaaga ttggcattaa atccacggca atcctgttcg gccaatacga
     1921 taaattgatt attggtattt tgcagattgg cgtactggca ctgatggcga tcatcggtga
     1981 gttaaatggc ttaggctggg gatattactg gtcaattgtg gtggctggcg cgctgtttgt
     2041 ttatcaacaa aaactgattg ccaaccgcga gcgtgaagcc tgctttaaag catttatgaa
     2101 taataactat gttggtctgg tactattttt agggctggca atgagttact ggcatttctg
     2161 atgatgtaaa aaagccggat gatcatccgg ctttcttctg ggttgcctga tgcgcggcgc
     2221 ttctcaggcc tacacaacac atcgcaattt attgaatttg cagattatgg aaggccggat
     2281 aaggcgtttt cgccgcatcc ggcaattctc tctgattacc cttcgccctg cgtcgcactc
     2341 tcaatcgtca aacgcacgtc tgatgtaatc aactccgcca gcaactgata aaccttcatc
     2401 gtttctgccg gttcggcatc gccgctatcg ctgatatacc cttcatcacg cagtgtcagc
     2461 accagagaac tgaacaccgc cttgtcgaag aactccggcg cgttgatgcc gtgcagcacg
     2521 gagagacgtt gcgcgacggt gcggctctct ttctccagcg taccgcggtt gatcgacggg
     2581 ttggcactca acaaccagaa ggtgatggca taacgttgca gcgtttcgcg cgcgcctgcg
     2641 gcgagcagct gtagcgtgcg agaatgcacc gggttgatat gcaactcatc atcttgcagg
     2701 gtaatcagcc cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg
     2761 tcgcgatccc agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc
     2821 tccatcaata cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa
     2881 ggcagcacca acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg
     2941 cgtggcagaa tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc
     3001 agcgcgtgat cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa
     3061 ggtacgttgc gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg
     3121 agtgagcgct gacgtgatgc cagtagcgca gtacagcaca ggttcatggc gtttgccgcg
     3181 cctgcgttgt taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac
     3241 caggccggac gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg
     3301 ttaaggtagg tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta
     3361 cgcagcttgc ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg
     3421 cgcagttctt tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga
     3481 atcagcgtaa tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta
     3541 ccggttttcg gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg
     3601 acggaataac cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt
     3661 ttattgcctt taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg
     3721 gccggccaga agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtgg
     3781 tacagcacgt aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc
     3841 agctcatgac cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc
     3901 tgataaagtc ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa
     3961 gagaaattcg ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg
     4021 gagatttttt tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc
     4081 agcttattaa acagatcctg acgagcaggc agacgtgggc ccacagcagc cagacgttga
     4141 cgggcaaagt gcatacgcgc cacgcgcgcc agtttctgag cgatagtttt atctgtgccg
     4201 tgttcatcgg ccatgcggcg cagagaaact gacggcgaga aacgcacaaa actgtcgcga
     4261 ccgagccaca gtacggcgaa aaatttttgt acgccgttaa gcatacgcag cggcgggttt
     4321 acttcgcctt tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc
     4381 acatccagat ttgggttgct gcggtgcaaa tcaagatagt cgtggaacag cttaatagac
     4441 tcttctttcg gcgtgtaata ggtgaacaca cgcggcccgc cgtgaatgaa cacatagcgc
     4501 ggcagtagcg tgccgtcgat ttccagcggc tctaacgggt caggcaagtc atgagccaga
     4561 cactgggcgc gcaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt
     4621 ggacgagagg tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt
     4681 accaggatgc ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata
     4741 aacgatgtaa agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg
     4801 ggaaattctg tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga
     4861 tttaacggta cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa
     4921 taccactgga ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc
     4981 tgcatggatc aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt
     5041 catcgcctgc tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat
     5101 gctggtgatg attgtggaaa tcctcaatag cgccatcgaa gcagtggttg accgaattgg
     5161 ctctgaatac catgagcttt ccggacgcgc aaaagatatg ggatccgccg cggtgctgat
     5221 tgccattatc gtcgccgtga ttacctggtg catcctgtta tggtcgcatt ttggataacc
     5281 cttccagatt tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt
     5341 tgctgtatat actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa
     5401 cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc
     5461 cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag
     5521 aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg
     5581 ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg
     5641 gtgaaccgct tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt
     5701 tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca
     5761 ttatggatgg tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg
     5821 ttgtcgcacg tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag
     5881 tcgaactgtt gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga
     5941 gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata
     6001 tctctgagac cgcgatgccg ccaggcgtcg cggtttgttt ttcatctctc ttcatcaggc
     6061 ttgtctgcat ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca
     6121 tgattttctc caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc
     6181 atcttgatag cccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc
     6241 tctttatgct gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg
     6301 gtgccaaaaa tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg
     6361 gggctggggc gttaattgcg ctgctgcgta caccgattat cgatctggcg ctgcatattg
     6421 ttggcggtag tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa
     6481 gcgctccggc gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg
     6541 cccgtgcgcc agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct
     6601 ggctggtgat ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg
     6661 aatatgcaac attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa
     6721 tttccggcga aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta
     6781 accgcgatat catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac
     6841 ttggtgcgcg actggggagt gacattgtcg ctgttaacgc ggttctgatg acgctactca
     6901 cctttaccgc ctatgcgctt gatggttttg cctacgcggt tgaagcgcac tccggtcagg
     6961 catacggtgc gcgcgacggt agccagttgc tggatgtatg gcgggcagcg tgccgccagt
     7021 cggggattgt agcgttactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg
     7081 cgttgctgac gtcgttaacc cagattcagc agctggctga ccgctatctt atctggcagg
     7141 tgattttgcc gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa
     7201 cgcgtgccgc cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc
     7261 tccttacgct gccgtggctg ggtaatcatg gtttgtggct ggcattaacc gtctttctgg
     7321 cgttgcgcgg gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt
     7381 ttgccgcaac gtgacggtta aaaattctga ataaataatc ctaagccaaa ttgctgacta
     7441 cacttaatct cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag
     7501 gtcttgatta tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg
     7561 aaagagcaat ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat
     7621 caactggtcg gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag
     7681 gtcgtggatt gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt
     7741 gtacaaggat gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc
     7801 tggcactgtt caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt
     7861 gcttcaatca cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg
     7921 tcttccacgc cttctgcaca ctcttctttc accgcgccgc agcgatcgca aataaacatg
     7981 gctgacgtat gggtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc
     8041 accttatgca caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc
     8101 ttggcttgcg gttcagcttc gcgcagtaaa tcaagcagat cataagcgct gatagcgcca
     8161 tcctgcagac tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc
     8221 tgcgcgcaga ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg
     8281 cacctcaaag tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag
     8341 aacccttatt gttgccgtaa tgttgatttt ctgttttgta ggtaaggtgt tgtgttgcct
     8401 tgtcgtacca ttatcaacac gataataatt aataattcat ttttaaataa attgcttcaa
     8461 gttaactcgc tatttattta actgggttta aacgcatacc tgtagttcag aaattatgag
     8521 cgatttttga tagtgaccag atggcaaaag aattataagg aggacacggt acgacaaaaa
     8581 aggtagatga atcatgttga agataatacc cggtgcaact ggatacttta ataaaacact
     8641 caattcaaat caattcgata atgaagatgc aatcaaagat aaattagata atagaggttc
     8701 aatcaaaggt aaataatata tatggtaaat ctattgatta tgcagcgctg cgtcatcggg
     8761 atataattat tgcaaaaata gatttgttta ttcaacgaat cacgcataat ttatggcatg
     8821 cacgtaaaaa aatgtgtttt taattgagca aataaatgat ctcaaaatgt gggtcaataa
     8881 gtatattgac gattgcactg atgaggattt aaacgatcgt gactttattg catcagttgt
     8941 tgaccgggct atttttcatt tcgcgattaa tagtatatgt aatcctgggg ataataaaga
     9001 tgcaatgccc attgaacaat gtacttttga tgtagaaaat aagaatgacc ttccctccac
     9061 ggttcagcta ttttatgagg aatctaagga taatgaacct ttagcgaata tacattttca
     9121 agcaataggt tctggttttt taacgtttgt taatgcctgc caggaacatg atgacaacag
     9181 cttaaaatta tttgcttcgc tgttaatttc actttcatat tctagtgcct acgcagattt
     9241 atcagaaaca gtgtatatta atgaaaataa tgagagctac ctgaaagctc agtttgaaaa
     9301 attatctcaa cgtgatatga agaagtacct gggagagatg aagcgtctgg ctgatggggg
     9361 agaaatgaat tttgatggct atctggataa gatgtcacat ctggtgaatg aaggaacgct
     9421 cgatcctgat attttaagca aaatgcgaga tgctgcacca caattaatta gcttcgcgaa
     9481 gtcgtttgac ccaacctcaa aggaagagat taaaatactt acagacactt ctaaattaat
     9541 ttatgatttg ttcggggtta aatcggagaa ataatatgtg aagttcttcg atagtatgga
     9601 aggcattata taaaaggacc caatatttat tgggttcttt tttctctatc aatgctatta
     9661 gcagggagat atatcaccag agtttaatgt gtgatttttt atttatcgtc gaacctggat
     9721 tgtttatcat tggccttaac aaagttaacg gctaataaga ttatttccat cacttcgtga
     9781 gagcttcatg ccttgagagg atctcaattt tcttttgcaa tgagacaggc gcttcctgtt
     9841 gttatggtat agtaccccgc tattgagcct cctgaatagt gatgctgaat aacataaccc
     9901 catgatatat cgataaaata atctctacat ttgaaaatgc acggtaattc tgaaatgcaa
     9961 aaaatcaacc aaaccagcgc aatgcctgaa aaaactgacg ttcactggag tggtcggttt
    10021 agcgttgcac caatgctcga taggatgtac cgtttttgaa aaacaaatag ttatatactt
    10081 tgtgggagcc tattgggaac ccggcgtttt catttcaagg tgtaatccat acgcggctta
    10141 agaatgagat ataatgcgac ttttagtgtt ccgcttgaga ggccatgatg cttaccctgg
    10201 acgagatagg tcaatcagta cgtaacaata tccagttgat tattgatcat gtcggctctt
    10261 gctgttggtc cgctcagtga tgatgattac aagattctgt gtggtggcta tggtgagctt
    10321 gaatgggact atgcgttaag tacctatggc aactccagag aaaagtatga gttctgcata
    10381 aaacttgttc agcaaggtcg ggttcaggga ataccatcag gagcagcaat ttgtgtttat
    10441 ggggttgaag aaaacatctt tcgtatccat atgatcgaaa ggttttctag agaagatgaa
    10501 tctcacccat tgaaagggcg catggtttta ctcactctta tgagtgcttt tatattttgt
    10561 aaagctgttg aatgtaaagt tgtccacatt gtagagccag taccagaact ggtgcagtat
    10621 tacgagtctt ttggtttccg catggaacag tgcggttatg tgatgtcggc agtcattgat
    10681 gagctgcagg atatctttct taaatttgct cagtaggtat agacgagaag ggtctacaaa
    10741 ttgtaggata cccgtccaga ttaccttaaa ggtacatcta tggcagtcgt tttgtgctta
    10801 aactactaag aaacgatgtc accaatcgac atgatcgatt ggcataagtt agcgaaacaa
    10861 gctagcttta aagaaagggt tagagacgcc tttactgtct cgggagtttt ctatgaaaga
    10921 tcaaaaagca accaagccac aggttaagtt cgacacaatg aaagcattcg caggtatggg
    10981 tgctgctgtt gaagttctga tgaaggctgc tcctaatgcg ttcactcacg ctactgtctc
    11041 tggtaaagag cagcagggta agcttcgtcg tcgcaaagca gcatgatcat agctggtgct
    11101 ttttgaaaac ccgccttcag gcgggttttt tctttagtga tgttctttgt ccttctgttt
    11161 gactgttctg acctgttccc actcgatacg tccttcttct cgccttttgt ctatgtattc
    11221 cgcaagatct tgaatattga tgcaacgttt tgctttttgt gatgtgccga tgcgatatgt
    11281 tggaacgggc aacttacaag cgtttgcttt tgcttctgcc gtggctggac tcataccaaa
    11341 gtacttttgg ctaactgctg agagttcaat gtttggggta ttgaattcag ccatcagtaa
    11401 aaacaaggtg ttcataattt tctccatcaa aaccggctgc acccgggaaa atcataattc
    11461 tgtgctggtg gcaggaatta atttctgcca gatagcggaa acatattttg cctgatgacg
    11521 ggcatcagcc agggcgttgt gccgttcgcc atcgaaaggc atgtccattt tggggtcgaa
    11581 tccgatggaa cgcccaagcg taacgatcgt gcgtacatcg tggtcattcc agtacgccca
    11641 cgggcagatt tgtcctgctc gctcgtaagc tccacgtaaa attacgttgt cgaaggtggc
    11701 cccgttaccc cagactttta aatatttcgt attgtctgcg tgccggttaa tgaaatggtt
    11761 tagttctgag agagcatcgc tgatcgacaa agtatcatca atacagattg cagctcgcgc
    11821 ttcagggctt tgtttcaacc accacaggat ggtatcgccg tcaggtgtag ctccttgctt
    11881 catagcactg tctaggctga caaccgcata gaattcttgt ccgatgtctc cggtttctgg
    11941 agtgaagaac accgcgccaa tggaaacgat cggtgcatcc ttatttttcc ccatcgtctc
    12001 aaggtcgatc attaagttgt tcatcacttc acctcctgcg gcggttccgg tagcggcatc
    12061 cagtgagttg cttgctcaat accattaccc ggcttaatcg ttgcatctcc gcgccgaaag
    12121 gtgcttccgg tatagcgtgc ggagcatatt agcggttcaa ccagagagct atcgaaattc
    12181 accgaaataa gcacgttctg gcccttttca ggcattcgat cactacagct tatccaacta
    12241 tccggagttc ccggagagtt gccatttaca tcgaagtttg gctctgcgtc ctgaaccaag
    12301 aggatgtaac cattcttggc tgtatcaagt tctaacgctt cggtgacggt gccgaaatag
    12361 cgattaccta aatcagcatc acaagtgctt atatcaatgg aaacttccat gccttcgatt
    12421 aattctggca agttgtaagt ttggcttaca ggttggctac cctgaagcat ggcggcgcgg
    12481 tgacaccaga taatccagcc aagcgccata tcccatgcca tgtattctct atcgccattt
    12541 tttgccctac ggcgatctac agattccccg aaacgcttct ccataaataa ttcataggct
    12601 gctcgttcat ccgatactgc tgccagtgat gccagtgtaa tttttaatgc ggtaagcatg
    12661 ttgttttgat cttcatcgag tccgaacggt atttcatccc gtgctgactc aatgctggta
    12721 atcgtgttct gtaaccattc tttggtaaga gtattcataa ctatttcacc ttaatctcaa
    12781 catttcgcag ctttagctct actggcaagt ctgactttcc tgttaatgct aatgcgagat
    12841 tttcaggagt aatgagagct gttattgttt tccccatcgc cagacgaata atcattcgta
    12901 tctcgcgatc gtcacatgct cccggtcgaa caattgatat ttgtccgttc atctcactct
    12961 cctttgatgc caatgtttac agcctgacaa gcctctttga gcactcagtc aacagcgtct
    13021 ttccatgctc cggtttcgac tggcggattt tcactcttaa cctgttcata gaagcgcacc
    13081 gctttaatca atccttcggg tgtcagtggc acaggctggg ccgtgaataa cgcctgaatt
    13141 tcatagttcg gcctgtcgtt gcaatcctct tttgtcggta catatttcca gccaccaacc
    13201 cacggcttct cctgaaagtt cgtaacgcct tttctcacgt agcgatatcg ccatgtcacc
    13261 ggttcggctt ccagcgatac cagcgcgact ttaaatgcgg taagtacgtt tttaaccaca
    13321 tcgattttga atactatttc atcacataca aacgatttat cgtctactac cgtttcaatt
    13381 ccggtaatcg tgttttgtag ccatttggtt aattcagcca tttttcatta ccgccctttc
    13441 gggcggtctc ctgatgttct gagggtgcag gaatccctcc ggttaaggat taaattttat
    13501 ttacagaact gaatttaatt attcagatat acgtatctgt aaccttacga acctactcac
    13561 tggatgccta tttcataaaa ataatccagt gggttttatc gttttttcct gttcgttgac
    13621 cgataacagg ttttctgtcg gtcagtacca atatctggcg aacaggtatt tgcgtttcat
    13681 tccatttaaa aatcagaacg ccggatggac gcaacacacg aaaggcttct ttaaatccct
    13741 gccgcaaatc atcacgccag gtatctttat tcagccgtcc atatttcttt cccatccagg
    13801 cgttatcacc aacacgctca agatgcggag ggtcgaatat aaccatcgaa aaagatgcgt
    13861 ctgcaaatgg tagtgcacga aaatcagcta tcagatcagg actgataatc aggcgtcgtc
    13921 catcacacaa tgtgtgctct tcctttctga tatcgctaaa tatcgcccgg tcgtcattct
    13981 tatcgaacca gaacatgcga ctgccacagc acatgtcgag gattgctgca tgtccagtca
    14041 ctggctgcct cctttgcgaa gccgttccgc ccattcttca agggatttct ctgcatattc
    14101 accggacagg ccatcaatcg gatgcgattc attagctaac tcttcttttg ctgaca
//
LOCUS       DAZUFB010000123        14052 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZUFB010000123 DAZUFB010000000
VERSION     DAZUFB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.123"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..955
                     /gene="hlyE"
                     /locus_tag="ACHY3M_004151"
     CDS             44..955
                     /gene="hlyE"
                     /locus_tag="ACHY3M_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFS4318962.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1162..1608)
                     /gene="ycgN"
                     /locus_tag="ACHY3M_004152"
     CDS             complement(1162..1608)
                     /gene="ycgN"
                     /locus_tag="ACHY3M_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFS4318963.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1700..2359)
                     /gene="ycgM"
                     /locus_tag="ACHY3M_004153"
     CDS             complement(1700..2359)
                     /gene="ycgM"
                     /locus_tag="ACHY3M_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS4318964.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2431..2724)
                     /locus_tag="ACHY3M_004154"
     CDS             complement(2431..2724)
                     /locus_tag="ACHY3M_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFS4318965.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2736..2894)
                     /locus_tag="ACHY3M_004155"
     CDS             complement(2736..2894)
                     /locus_tag="ACHY3M_004155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318966.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2965..3366
                     /gene="pliG"
                     /locus_tag="ACHY3M_004156"
     CDS             2965..3366
                     /gene="pliG"
                     /locus_tag="ACHY3M_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFS4318967.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3469..3837)
                     /gene="ycgJ"
                     /locus_tag="ACHY3M_004157"
     CDS             complement(3469..3837)
                     /gene="ycgJ"
                     /locus_tag="ACHY3M_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFS4318968.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4357..5052
                     /gene="minC"
                     /locus_tag="ACHY3M_004158"
     CDS             4357..5052
                     /gene="minC"
                     /locus_tag="ACHY3M_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFS4318969.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5076..5888
                     /gene="minD"
                     /locus_tag="ACHY3M_004159"
     CDS             5076..5888
                     /gene="minD"
                     /locus_tag="ACHY3M_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFS4318970.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5892..6158
                     /gene="minE"
                     /locus_tag="ACHY3M_004160"
     CDS             5892..6158
                     /gene="minE"
                     /locus_tag="ACHY3M_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFS4318971.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6530..6874)
                     /locus_tag="ACHY3M_004161"
                     /pseudo
     CDS             complement(6530..6874)
                     /locus_tag="ACHY3M_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6909..7028)
                     /locus_tag="ACHY3M_004162"
     CDS             complement(6909..7028)
                     /locus_tag="ACHY3M_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4318972.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6989..7174)
                     /gene="ymgJ"
                     /locus_tag="ACHY3M_004163"
     CDS             complement(6989..7174)
                     /gene="ymgJ"
                     /locus_tag="ACHY3M_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFS4318973.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7275..7448
                     /gene="ymgI"
                     /locus_tag="ACHY3M_004164"
     CDS             7275..7448
                     /gene="ymgI"
                     /locus_tag="ACHY3M_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFS4318974.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7450..7794
                     /gene="ymgG"
                     /locus_tag="ACHY3M_004165"
     CDS             7450..7794
                     /gene="ymgG"
                     /locus_tag="ACHY3M_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFS4318975.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7798..8133
                     /gene="ymgD"
                     /locus_tag="ACHY3M_004166"
     CDS             7798..8133
                     /gene="ymgD"
                     /locus_tag="ACHY3M_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFS4318976.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8190..8714)
                     /locus_tag="ACHY3M_004167"
     CDS             complement(8190..8714)
                     /locus_tag="ACHY3M_004167"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4318977.1"
                     /translation="MKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQ
                     PQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPF
                     VEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNG
                     YNDTAVMVGLKYKF"
     gene            complement(8711..10510)
                     /locus_tag="ACHY3M_004168"
     CDS             complement(8711..10510)
                     /locus_tag="ACHY3M_004168"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4318978.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKAHTLTPGRNIAGLKTR"
     gene            complement(10627..10836)
                     /locus_tag="ACHY3M_004169"
     CDS             complement(10627..10836)
                     /locus_tag="ACHY3M_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318979.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11236..11454)
                     /gene="ymgF"
                     /locus_tag="ACHY3M_004170"
     CDS             complement(11236..11454)
                     /gene="ymgF"
                     /locus_tag="ACHY3M_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFS4318980.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(11586..13109)
                     /gene="pdeG"
                     /locus_tag="ACHY3M_004171"
     CDS             complement(11586..13109)
                     /gene="pdeG"
                     /locus_tag="ACHY3M_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4318981.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13441..13689)
                     /gene="ymgC"
                     /locus_tag="ACHY3M_004172"
     CDS             complement(13441..13689)
                     /gene="ymgC"
                     /locus_tag="ACHY3M_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFS4318982.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(13802..14050)
                     /gene="ariR"
                     /locus_tag="ACHY3M_004173"
                     /gene_synonym="ymgB"
     CDS             complement(13802..14050)
                     /gene="ariR"
                     /locus_tag="ACHY3M_004173"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFS4318983.1"
                     /translation="MHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLSGDNVNN
                     KNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         4032 a         3249 c         3001 g         3770 t
ORIGIN      
        1 aggagtttca ttacaattta tatatttaaa gaggcgaatg attatgactg aaatcgttgc
       61 agataaaacg gtagaagtag ttaaaaacgc aatcgaaacc gcagatggag cattagatct
      121 ttataataaa tatctcgatc aggtcatccc ctggcagacc tttgatgaaa ccataaaaga
      181 gttaagtcgc tttaaacagg agtattcaca ggcagcctcc gttttagtcg gcgatattaa
      241 aaccttactt atggatagcc aggataagta ttttgaagca acccaaacag tgtatgaatg
      301 gtgtggtgtt gcgacgcaat tgctcgcagc gtatattttg ctatttgatg agtacaatga
      361 gaagaaagca tccgcccaga aagacattct cattaaggta ctggatgacg gcatcacgaa
      421 gctgaatgaa gcgcaaaaat ccctgctggt aagctcacaa agtttcaaca acgcttccgg
      481 gaaactgctg gcgttagata gccagttaac caatgatttt tcagaaaaaa gcagctattt
      541 ccagtcacag gtagataaaa tcaggaagga agcgtatgcc ggtgccgcag ccggtgtcgt
      601 cgccggtcca tttggattaa tcatttccta ttctattgct gcgggcgtag ttgaagggaa
      661 actgattcca gaattgaaga acaagttaaa atctgtgcag aatttcttta ccaccctgtc
      721 taacacggtt aaacaagcga ataaagatat cgatgccgcc aaattgaaat taaccaccga
      781 aatagccgcc atcggggaga taaaaacgga aactgaaaca accagattct atgttgatta
      841 tgatgattta atgctttctt tgctaaaaga agcggcaaaa aaaatgatta acacctgtaa
      901 tgagtatcag aaaagacacg gtaaaaagac actctttgag gtacctgaag tctgataagc
      961 gattattctc tccatgtact caaggtataa ggtttatcac atttaaaaac acagacgttt
     1021 tttaaagatt gagagttacc tgttgataat aaggtatcgg gtaactctca ttatgacaga
     1081 gcgctttcaa aaacagggct acgaaatggc gagcatccag acgtatatta agaaagcccc
     1141 cataaacatc tattaaattt atcactgcgc ccagtcaggt ttatttaaga tatgatcctg
     1201 ccagtcaatc acttctgatt ctttcactgc gatatgacgc acagagatac gttcaccatg
     1261 cattgccgct ttcgaacccg taagtagcgg atgccacgca ggtaaatctt taccttccgc
     1321 cagcaaccga taagcgcagg tcattggcag ccattcgaat gttggcagat tttcacgggt
     1381 taatttaatg cagtcgggtt caaactcgaa acgacgttcg tagttccgac attgacaggt
     1441 tttaatattg agctggcgac aggcgacgtt agtgaagtag atttcgtcgg tgtcttcatc
     1501 catcagttta tgcaggcaac actgaccgca accatcacac aacgactccc attccgcatc
     1561 gctcatttcg tccagggttt tactttgcca gaaaggtaca tcgctcatca ggtgttccgc
     1621 cattacgtta aaacgcacct tataaccagt ctggcacagc gatgcaagtt ttgccgccgc
     1681 tttcaggcgg caaaaagtat tacaaaacgc gagttgccaa agaatgccca tcgaaagtga
     1741 ctgtcagctc atcaccgctt tgcaacgggc cgacgccatc aggcgtgcct gtcagcacaa
     1801 cgtcaccggc cttgagggta aaaaacttgc tcatataagc gatcagcgga acaattttat
     1861 ggatcatgtc cgcagtcgta ccttgctgac gttgctcgcc gtttacgctc aggcccagcg
     1921 ttgtattttg cgggtcgccg gtaaattccg ccgcgggaat aaacccggaa agcggacaag
     1981 agttatcaaa cgctttagcc ttttcccacg gctgcccggc tttcttcatt tttccctgaa
     2041 catcacgcaa tgtcagatcg agcgccacgc cataaccggc aatggctttg cggacatgct
     2101 cttccgtagc ctgacgcagc gtcgcgccaa tcaacaccgc cagttcgact tcatgatgaa
     2161 ctgaaccgaa atcggatggg atcgccaatg gctgccgcag atcgcacagt gccgtttctg
     2221 gtttaataaa cagcactggc tcttcgggca ctgcgctgcc catctcttta atatgtttgg
     2281 catagttact gccaacacag actactttac tcaccggata atccagcagc gcaccttgcc
     2341 agttgtgatg ttgatacata acgttcccct caccttatcg tggatatgaa aacccgcgcc
     2401 gacaaagata atgccaccgc cggatatcgg ttatttgtta gtgtcgtctg ttttctgccc
     2461 catgacggaa agatgttgct tcagcaaatc ttcgggtggt ggcggtaact gcaaatagta
     2521 accttgctcg gttaatgcca gtttaacctt ttcaatatca gcattgacca gtttcttccg
     2581 cccatccaac ggcagaatca tcgctaactg aggctgacca aaacctttca tcagttcctc
     2641 aggaacacgc gaaaaatcgt cttttttttc gacatataaa taggtctggt cacgcttgct
     2701 gcttcgataa atcacacaaa acatactttt actctttaag atgccaggtt ttggcataag
     2761 ctaacatcaa tgaaacccgc gccacaagcc agccagctgt agatttctac ggtttgttga
     2821 gtccatgccc ctttataagt aatccccccg gcaaaaatgc cgcgccggga ttttaccgaa
     2881 cgcaggaaag ccactggaat tctcccgtta gggattgtta tattataaca gttcatcgta
     2941 ctcattctga acaggagact accaatgaaa atcaagagca tcagtaaggc tgtattgctg
     3001 tttgcactgc taacctccac gagctttgct gcgggtaaga atgtcaatgt tgagttcaga
     3061 aaaggccata gcagcgctca gtactccggc gaaataaagg gatacgatta cgatacatat
     3121 accttctatg ccaaaaaagg tcagaaagtg catgtgagta tctctaatga aggcgctgat
     3181 acttacctct tcgggccagg aatcgatgat tcagttgatt tatccagata ttccccagaa
     3241 ctcgatagtc acggacaata ctcactccct gcttctggca aatatgagct gcgagtgttg
     3301 caaacccgaa acgacgcccg taaaaacaaa actaaaaaat ataacgtcga tattcaaatt
     3361 aagtaaatgt caaccgacgg ggactctccc cgtcataaac gttaacgccc cactcctctc
     3421 tgtcgctcaa gaggttaatg acgaatagaa cataatgtaa acaagacatc actcacgaca
     3481 cataaataac atctttgtgg tggtttgatt gatttttcca ctccgcttac catctacacc
     3541 aaaataacga tcatcacgac ataatttttc tttaacatcg caaaatacac cgttagcgaa
     3601 tgtaaattcg ctgggatcgt agccttgtaa tgattgtaag ttttcagcgg ctttttcgcc
     3661 gagatatttc cttgttaact ctggggaaat gccatctgag ttcacacaaa tatacgggtc
     3721 gcaaacaacg ccgcacgccg gagattgaag tccgctagcg tcatgtccag acgccattga
     3781 tgagaacatc atccccacac ctgacaatcc gatataaata atacgcatca tattcatagc
     3841 acatcctgat aatccaaccg tacaacactt atgctcattt cgtcatcaat ccgtaatcaa
     3901 aaccgctgac ctggacgagt tacgatgacc tgacagaagt atcagacgtc ttctgcggtt
     3961 tctatgatag agcaattctg tggcggatat taagataacg ggtatctgtt ctttaatgat
     4021 gccgcaaaaa atggaaagtt aagaaagcct ggcgtggtga aaaaatacac ttgcctcaat
     4081 actggatatt taaacaggtc tggagatgct gtcacgatta gggtgcggaa aaaggaagcg
     4141 taaataagaa gaatcaaaag tacgacggca atgggttgat tgacaagggt attttttaag
     4201 ctatgaatca gcgccattta tcacagaata gacttttact ctgaataaat gggagggtga
     4261 cttgcctcaa tataatccag actataacat gccttatagt cttcggaaca tcatcgcgcg
     4321 ctggcgatga ttaatagcta attgagtaag gccaggatgt caaacacgcc aatcgagctt
     4381 aaaggcagta gcttcacttt atctgtggtt catctgcatg aggcagaacc taaggttatc
     4441 catcaggcgc tggaagacaa aatcgctcag gcccccgcat ttttaaaaca tgcccccgtt
     4501 gtactcaacg tcagtgcact ggaagacccg gtaaactggt cagcgatgca taaggcggtt
     4561 tcggcaaccg gtttgcgggt tattggcgta agtggctgca aagatgcgca acttaaagcc
     4621 gaaattgaaa aaatggggct gcctatcctg acggaaggaa aggaaaaagc gccacgtcca
     4681 gctcccgcac cgcaggctcc agcgcaaaat acaacgccgg tcacaaaaac gcgtttaata
     4741 gataccccgg tgcgttccgg tcagcgtatt tatgctccac aatgtgatct gattgttaca
     4801 agccacgtta gcgctggggc cgaattgatt gccgatggta acattcatgt ctatggcatg
     4861 atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg aaacgcaaat attttgtacg
     4921 aacctgatgg cggaactggt gtccatcgca ggtgaatact ggctgagtga tcaaatccca
     4981 gcagaatttt atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa
     5041 ccgttaaatt gatccctttt taacaaggaa tttctatggc acgcattatt gttgttactt
     5101 cgggcaaagg gggtgttggt aagacaacct ccagcgcggc catcgccact ggtttggccc
     5161 agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga
     5221 ttatgggttg tgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa
     5281 cgctaaatca ggcgttaatt aaagataagc gtactgaaaa tctctatatt cttccggcat
     5341 cgcaaacacg cgataaagat gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc
     5401 tgaaagcgat ggattttgaa tttatcgttt gtgactcccc ggcagggatt gaaaccggtg
     5461 cgttaatggc actctatttt gcagacgaag ccattattac caccaacccg gaagtctcct
     5521 cagtacgcga ctctgaccgt attttaggca ttctggcgtc gaaatcacgc cgcgcagaaa
     5581 atggcgaaga gcctattaaa gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa
     5641 gcagaggtga catgctgagc atggaagatg tgctggagat cctgcgcatc aaactcgtcg
     5701 gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc
     5761 tcgacattaa cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt ctgttgggag
     5821 aagaacgtcc tttccgcttc attgaagaag agaagaaagg cttcctcaaa cgcttgttcg
     5881 gaggataagt tatggcatta ctcgatttct ttctctcgcg gaagaaaaac acagccaaca
     5941 ttgcaaaaga acggctgcag attattgttg ctgaacgccg tcgcagcgat gcagaaccgc
     6001 attatctgcc gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac gtacaaattg
     6061 atcctgagat ggtaaccgta cagcttgagc aaaaagatgg cgatatttct attcttgagc
     6121 tgaacgtgac cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa aacgcattta
     6181 tcttcaaggc agagtcattt ctgccttgag tttttcatcc ctctcatcca cgttgtggta
     6241 aagcggcgag tattcttgct gatactcctc attgctattt ccacctcccc ctttacacct
     6301 taaggctgtt cccacgcaca gaacaaaatg tttatgcctg gttgagtaaa taacctcatt
     6361 gttagtgtgg atacactggg ggatgataac cccgcaattt cattattcat tgttctccca
     6421 taggatgagg tttcccgccg taactggcgg gctttttttg ccccagaatt ttccctttta
     6481 gccccctgta aacgaaaact gcgccgaagc gcagtttaat cgttgatggt cagaacgacc
     6541 agttcacacc agccaccgca ttccacgggg attccacacc ggcaccatgg ctatacccca
     6601 ccccgaaatg cccgcttaac gtactgctga atgaggcttt aatacctgcc tggtatattc
     6661 cacgtctgcc cgacaaatca ttgacaaaat taccgtcact attcactttc acccgattat
     6721 catcgacaaa ttctttgcgc acagccgcct tcaggcaggg ttcaatttcc ataccattcc
     6781 ccagacgcat gttgtaactc agcgttgcac ccagttcacg atatatactg cgggtatcga
     6841 ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac ctaagctctg
     6901 ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt
     6961 gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa
     7021 agaggcattt tttacacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca
     7081 gataatactg tcgtacgctt atctttcgac aatgaggccg tcggcaaaat aaaatgacta
     7141 atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat aatattgata
     7201 aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt cttgaataaa
     7261 aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac gtaaaaaaca
     7321 taattcctat catcacagcc acgctcattt tgaattatct gaataatagc gagcgttctt
     7381 tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg
     7441 acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc
     7501 acgccagcga tggcggatat gaatcgcacc acgaaaggtg cattgctagg cgcaggcgtt
     7561 ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt
     7621 gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagta
     7681 ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt
     7741 aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg
     7801 atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat gacctgggcg
     7861 caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc
     7921 aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat
     7981 gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag cgccaaagtg
     8041 gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt
     8101 gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat
     8161 acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc caccatcact
     8221 gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca gacgctggcc
     8281 gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact cgccccatct
     8341 tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg gatccagttc
     8401 atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga
     8461 ataaaggtgc ggaaaccaag tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt
     8521 gagccattgg cttcggtgtg cgcattatga tcgacgccca tccagatagc ctgtgcctgt
     8581 ggctggaata tccacgtgta tttcgccgct tccagaccaa aggatttatt cagggcaaag
     8641 ccgtaacccg cttccagcga gacggttgcg cctttcgcac tataggattc ataggctaat
     8701 tcatcccctt tcaccgagtt tttaaaccag ctatattgcg cccaggcgtc aatgtatgcg
     8761 cttttttact gatgtcatcg gcaaaccaag tggcatacag ccctgcgcaa tagcctctga
     8821 cactcccttt cgaacgataa tccgacacgc tggaatgagt tgagttgtag tcgcgggcat
     8881 aaccagccat cactcccaaa cgccaactgt cactgtcggt aaaaccaccg gttaacagct
     8941 cggcccctaa ctgcgagacg tagcgatgcg aggtcgttct caactgtccg ttgctgtcac
     9001 gccaggcatt atgcccgcca atttgacgta gccaaagtcg gcttgaacgc tcctgttcag
     9061 ttacaggatc gatgtagcgc atttcacccg cacggtcgtt cagacgcata acaaaaagag
     9121 tgttagcggc tgccaggttg gcgacgtaac ttccagcttc gaggcgcagg acgggggtag
     9181 gcgcaacagt aggcgctgaa cttcctccgg agtttgtttc accgccgctc gttgcctgga
     9241 agttagtcag ataccagttt ttattggtcg cttcggtatt acctcgggtt aatgaatatt
     9301 cgtaggcacc ggcgaaaatc ctcgaatcct taataaattc cccatttgat tccccctcaa
     9361 cgctgataat ctctatccca tttaacgtgt aagctccgct gcctcctgca ttggtaactt
     9421 taactcgggt tgttccagag gtatttccat taataacgac tttatccgta gctgaattat
     9481 catcacccaa ttcagttctg agatgcaata cgccattatt accaacgtaa ttttcagtta
     9541 ttgttaatct tgttggttcg acatcccgtc catctgcccg ggaaatatag accgtactat
     9601 tatcaaccgt gaggttgctc accgttgagt cagctttaat atcccaggta ctattatctg
     9661 aaagcgacag ataggtatga gtgttaacat ctgttgaaat attagctgaa cccgttattt
     9721 ttgaattagt tgcatcgacg gtcaaatgac ctttaatatt attgataaaa acatctgcag
     9781 ccgatgatac ctcactatcc gtaatggtca cgttactcgt ctcattttta tggtagagga
     9841 tttcatttgc tgaccctagt gagctaccag ataaaatatc taccgttgct tcctgaccat
     9901 ttatggaaga ctccagcaca attccagacc cctgatgcgc gactgcagat gtatttttca
     9961 gcgtcaccgt gccgccatta taaattttag cagtcgcggc gtcaggaccc gttgtataaa
    10021 tttctccctg gttaaatgaa actgtcccct ggcgggcagc gatggcatga gcattgtccc
    10081 catgcgtggt gatatcaaca tcggttaatg taacgctggc attggtataa tcaagcgtgt
    10141 caccagcctt gacgacagtg ccattagcgt acgcggcata actttctgtt ccgtgggttg
    10201 tgatctcccc ttttgccata tccagttttg ctatggcatt attttcatta cttcctgtat
    10261 cggtagtact ttcttcatca agagaagata cacacgcacg cgcgcttccg ggagatgcac
    10321 acgcaagaac gccgactgca tgattacccg cagtaacaat aaatgtatca tctccgtcag
    10381 aaaaagaggg ggtaaccgaa ccgtcagagt tgactcttat tatggtgtca gtattcaggt
    10441 ttccactcgt ttgacttagc aaaccaattg agtaatcccc cgttatgaca atcgtgtttt
    10501 taccctgcaa aataacagca ccgccaaatt cacttacgat tgcgctagct gttaatgctg
    10561 tgcttagagg actattgtca gaaggatata aaccgcccgt tgttacaatg acgccattgt
    10621 taatggttaa cttgctaccc tctcctgcaa cggatacccg taacttccat tacccgttgt
    10681 tataactagc gcattatcaa tatcttttac ttttccatct tttattgcca cagcatgttt
    10741 atattcatcg tgctgaataa actgttcagc atatgcatta cccaccgcca gagcaataag
    10801 tcccaaacta aacccggaga gttttttcag tttcatagat taaccatcca ttcattggat
    10861 taacaaacat tcctggtcaa ataacctatg cgttcagatt accaaaacgg agggtaaaga
    10921 caaaaacaaa agcacaaaaa atttaaatag ataaacagcg acaatatata aatgataaat
    10981 aagtcggcat tatcattttt caccttattt tcatgacatt gatcactttg aggtgacgat
    11041 tttgtttaac aaataactct tcgacatcac aatcattgat agttgcaata aaaattattt
    11101 caatgacata taaaaacaac tgtgataaca ccagggttga caactatttg cgcaataatt
    11161 cctttacttc aatgatccag gcattcactg ccagcaaccc attcagccgc atactgacag
    11221 tgagaaactg aaagattatt gctgatccct gtggctgacg agtctttccc ttaatcgatt
    11281 aaccagattg atcgccagcc aaacaagggc attaagcaaa tttatcgtca caagcacttc
    11341 tcccagcccg gtactgggtt ttgcaataag gatgaccagg agggtgatca gcataaatgc
    11401 aatggaaaag atgatataga gtgtgaaata atccagatta ttactattgt tcatgctact
    11461 aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa caaaataatg aacaatagta
    11521 acaagaggca acgagctaat tagctgtcta taattacacc gcactatggc cttactgatc
    11581 aattttcact caaccacaac cttcaccttc ggtttagaaa ggataatttt gaccaggtcg
    11641 atgtatgtaa caggtttata gaaataataa ccctgctgaa atgtgatatt attttggttc
    11701 agatagtcaa gttgttcttt cgtttcgaca ccttcagcga cgatactgat cgaaagttta
    11761 cgcgccagat ccaatacaca atccagaatt cgggttgatt ctgggtctgc gtttacgcgg
    11821 ccaacgaaac tgtgatcaat tttgatataa tcaatatgca ggtcatgaag ataagaaagc
    11881 cctgagtagc cagtaccgaa atcatccagc gcgatgacaa aaccattttc atgcagaatg
    11941 ttcaaccgct gaaccagact ttcatcaaca ttcaatggct cacgctcggt gacttccaga
    12001 acaaggttta aatcgcggcg ggtaaaactg tcacggaaat ttaaacactc gtcgacaaac
    12061 gtcggcgaaa taatatgcga agcgctaaaa ttaattccaa tatgaaaacc ttccggcaat
    12121 ttactcgcga tagcgttcat ctgtctggca acctgattca tcaggctttg cgtaagcgga
    12181 acgattaatc cggatttttc agcaagtgga ataaatgccg cgggtgatat atatccaccg
    12241 tgaggttgtt tccagcgggc taacacctca actccccgca atgtcccttc ccgaccattt
    12301 accacaggtt ggtaaaaagg gacgatctcc ccattattta ttgcccgtcg caggatctct
    12361 tcaggcgttg cgcttttatt gaaatatcta tcaagcagga aggcagcggc gcaggcgatt
    12421 aacagaataa atattaaaat cccaaagcct ttatcgataa gtctgtagaa gctaaagagt
    12481 ggcggcgggt taaagataat agtgaaatga tattttgtgg agtgaacctt ctcggtatag
    12541 ggataatgtc cattaagcgt catcacatca cccgttggtc caataatgtc atccgccacg
    12601 cgtaatacat accttacccc tttcaagggt acattaagtg ccccacgaat atgctgatcg
    12661 cttatcgtaa ccaaaatgcg cgtatctgca aattggttct gatagagcag gataggtaat
    12721 ctattaacgg tgtcgattgc tggagccaac agtaaattac tgtccgggaa aacaggaata
    12781 cgagacaaca ggacccgatt cccaggcagg gatgtacacc agactattcc ctgtttgaga
    12841 atgatgattg tgcgcaggtg aggtttcaga gctgcttcag tgccaagctg atattgctcc
    12901 tcgaggttgc attccttccc ggcaatgtta accgctgttt gagtcgcttg tgacgcttct
    12961 tcaagtatat gattgatatt gttggcggca tatctcgctc ccgccaggta ctctgctttt
    13021 gcggaatacc agagctggat gttcaaaata gcgacaccgg taataaacaa gcatatcgca
    13081 acgaggatgg gtatgagtgt attgcgcatt tgcttgtccc ggccaaaggg tttaatacat
    13141 gaaaatgcca ttcattcgct cgcgctggca tctacagaga gcatggtaga gagcataaga
    13201 aatatccacg ccgaagataa aattttctcc atcttgatct aacccattaa ttattgagca
    13261 taatgtaggc agtacaaaat aagttaggcg ggatatcagg cgtcaagaat ggagagcgag
    13321 aactctccat tcttgacacc tgatattgcg gacataataa gaaagcataa cgcctgaaat
    13381 gctcactttg catcagcatg gtgatacagc tgatgtttat tctaaaacct tactcaagtt
    13441 ctaagagagc acggattccc tgtcattaat aatgatatcc gttagtgtag tcttatgcat
    13501 ttcaatgtac tgcaatattt gcagatagag atcagaaaga atcacactgg tttcatcttt
    13561 taaacgcaag cgtaaagcct gatcaataat atgactatca acatacccat gcgtcatgaa
    13621 cacctccctc tcgagatttt taaacaaagc acattgaaaa ataaatctct ctgggattga
    13681 attattcatg tcagcctcgt agaaatatta acccaataaa attatgctgt gtatagtcta
    13741 aagcgacaga caaaaataag atgaatgagt tttctataaa cttatactta ataattagaa
    13801 gttacatatc atcagctgtg tatcgcaaca cgatttccag tgtttttcta atcacatcag
    13861 ctttgagaat atcacttgtt gtttccaggg agtgtatcag acttaagata atatttttat
    13921 tattaacatt atcgcctgaa agcattaaat tggtgacagc ctgccctaac actgctgact
    13981 cttcttctaa caaattaccc gaactgcgaa agtaacttgc taacgcttta tcagttattg
    14041 cattatgaat tg
//
LOCUS       DAZUFB010000124        13995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZUFB010000124 DAZUFB010000000
VERSION     DAZUFB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.124"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..204
                     /locus_tag="ACHY3M_004174"
     CDS             <1..204
                     /locus_tag="ACHY3M_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS4318984.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     RGATYPVLIDSIAVEVNSTDTAA"
     gene            341..520
                     /locus_tag="ACHY3M_004175"
     CDS             341..520
                     /locus_tag="ACHY3M_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS4318985.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            561..833
                     /locus_tag="ACHY3M_004176"
     CDS             561..833
                     /locus_tag="ACHY3M_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS4318986.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            910..1125
                     /locus_tag="ACHY3M_004177"
     CDS             910..1125
                     /locus_tag="ACHY3M_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4318987.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1130..1663
                     /locus_tag="ACHY3M_004178"
     CDS             1130..1663
                     /locus_tag="ACHY3M_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4318988.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALSCWGIDR"
     gene            1938..2507
                     /locus_tag="ACHY3M_004179"
     CDS             1938..2507
                     /locus_tag="ACHY3M_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS4318989.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2507..2656
                     /locus_tag="ACHY3M_004180"
     CDS             2507..2656
                     /locus_tag="ACHY3M_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318990.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            2664..3128
                     /locus_tag="ACHY3M_004181"
     CDS             2664..3128
                     /locus_tag="ACHY3M_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4318991.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGYRSLRINATCPGP
                     VREATGTARVDNATGPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(3160..3453)
                     /gene="bor"
                     /locus_tag="ACHY3M_004182"
     CDS             complement(3160..3453)
                     /gene="bor"
                     /locus_tag="ACHY3M_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFS4318992.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            3603..3806
                     /locus_tag="ACHY3M_004183"
     CDS             3603..3806
                     /locus_tag="ACHY3M_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318993.1"
                     /translation="MSTALTGNFSAGVCGDNQKRCTPGFLIFHEMGAISREAACPVRW
                     WKKPDKTTALCKYRSNMVLLCEI"
     gene            3862..4668
                     /locus_tag="ACHY3M_004184"
     CDS             3862..4668
                     /locus_tag="ACHY3M_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS4318994.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4649..6355
                     /locus_tag="ACHY3M_004185"
     CDS             4649..6355
                     /locus_tag="ACHY3M_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS4318995.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6355..8499
                     /locus_tag="ACHY3M_004186"
     CDS             6355..8499
                     /locus_tag="ACHY3M_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4318996.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8657..9664
                     /locus_tag="ACHY3M_004187"
     CDS             8657..9664
                     /locus_tag="ACHY3M_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318997.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            9688..10902
                     /locus_tag="ACHY3M_004188"
     CDS             9688..10902
                     /locus_tag="ACHY3M_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFS4318998.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            10958..11347
                     /locus_tag="ACHY3M_004189"
     CDS             10958..11347
                     /locus_tag="ACHY3M_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4318999.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            11397..11858
                     /locus_tag="ACHY3M_004190"
     CDS             11397..11858
                     /locus_tag="ACHY3M_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319000.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            11842..12405
                     /locus_tag="ACHY3M_004191"
     CDS             11842..12405
                     /locus_tag="ACHY3M_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319001.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            12405..13055
                     /locus_tag="ACHY3M_004192"
     CDS             12405..13055
                     /locus_tag="ACHY3M_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFS4319002.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            13052..>13995
                     /locus_tag="ACHY3M_004193"
     CDS             13052..>13995
                     /locus_tag="ACHY3M_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFS4319003.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDT"
BASE COUNT         3631 a         3211 c         3973 g         3180 t
ORIGIN      
        1 ttccgctttg ccgggaataa cagcctgcag gtgacgccgg ttattgatgg tcaggatggc
       61 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      121 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      181 aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      241 ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct ggttttttaa
      301 tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt
      361 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg gataccgggc
      421 catgcgtgac ttctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag
      481 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      541 cctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgca gaggcagtga
      601 aggaagtcct gcgctctgaa gaagtcctga gcgcactgaa acaaaaactc cgccagaatc
      661 ttgaggcgcg tcttgatgca gaggttgatg ccattctgga tgaactgctt ggtgtacagg
      721 cagagccacc gactgaagcg ggagatacca ccgcagagag cggtgaagtt cagcctgaat
      781 caccggtcgc cgatgcgact gaacctcaac ccgaatcggt catgatgctg taacggggag
      841 tcagggccat cagtaaacag ctgctggcct ttttcatgtt gtgagcttcc ggattgcggg
      901 agacggggta tgtaccagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg
      961 gtgggaacgg gctactggtt cctgcagttg ctggacaggg tttccccgtc tcagtgggcg
     1021 gcaataggcg tgctggggag tctgctgttt ggtctgctga cgtacctgac gaacctgtat
     1081 ttcaagatta aagaagaccg gcgtaaggcg gcgcggggag agtaagctga tgagcaggaa
     1141 attccgctat ggtttatcag cggtagttct ggcgctgatt gccgcaggtg cttctgcgcc
     1201 tgaaatcctc gaccagtttc tggatgaaaa ggaaggtaac cacaccacgg cataccgtga
     1261 tggtgtgggt atctggacca tctgccgtgg agccacccgg gtggatggta agcctgtgat
     1321 tcctggcatg aagctgtcga aggaaaaatg cgaccgggtt aacgctatcg aacgggataa
     1381 ggcgctggca tgggtggcga agaacatcag agtgccactg actgaacccc agaaagcggg
     1441 tattgcgtca ttctgtcctt acaacattgg ccccggtaag tgtttcccgt cgacgtttta
     1501 taaacgaatt aatgcaggtg atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa
     1561 ggacggtggc agagactgcc gtatccgctc aaataattgc tacggtcagg tatcaagacg
     1621 tgaccaggag agtgcgctgt catgctgggg tatcgacagg taagcagaat attttgctga
     1681 aaaatgacgt tggccaacgc gggcagataa cacgaaatcc tgcgaactgg caaaatgtaa
     1741 gtgaataaag tcaacaagat tgtttcatga agaggcaccg taatggtgcc tttgtcattt
     1801 ctgcgcttcg cacaagcgta aataaaccaa agaacctttc aggatgagcc ctggtggata
     1861 accggcagtg gtctggttaa ccctctttgg gctggttatt cctgtgcgca gggttcatca
     1921 ctaaaaggaa ataaccgatg aatatgatga ccgtgccgtt tcacggcgat tctctttatg
     1981 tggttaacca taacggcgaa ccatacgttc ccatgaaacc tgtcgttgcg gggatggggc
     2041 tggcctggca atcacagttg gctaagttaa gacagcgttt tgcgtcaact ataacggaaa
     2101 tcgttatggt tgctgaggat gggaaacgac gcaatatggt gtccctgccg cttcgaaaac
     2161 ttgcaggctg gttacaaacc atcaatccca acaaagtaaa acccgaaatc cgcggcaagg
     2221 taatccagta tcaggaagag tgtgacgatg ttctctatga atactggacg aagggttttg
     2281 tcgttaatcc ccgtcgaatg agtgtgatgg aagaactcaa tcaggcttgc gctgacatga
     2341 aacgggataa aaacattgcc agtgtgtttg ctaccgggct gaatgagtgg aaacaggtta
     2401 aatccgcgca tgtatcaaaa atccgcacat tgataaacga agcgaatctg ctgattgatt
     2461 ttgtcctggc tgatacaggc aaagggaaaa taacaaaggc ggattgatgg agtggtggct
     2521 aatgatatcg gataaactca taacgctggc gaagatcctc tgtgtaatcg tcggcatttc
     2581 atttttagtc atgctggttg ccattttctt ttccaccgcc tggcgagtcc tgacgttatc
     2641 gggactggtg gggtgaaaga gagatgaacc gtgttctgtg tgtggtgatt attgtcctgc
     2701 tggtagcctg tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccataacct
     2761 acaaagagca gcgcgataaa aaagtcagtg agctggagct ggcaaatgca accattactg
     2821 atatgcagca gcgccagcgt gatgttgctg cacttgatgc cagatactcg agggaattag
     2881 ccgatgcgag agctgaaaat gaaactcttc gcgctgacgt tgccgctggt taccgcagcc
     2941 tgcggatcaa cgccacctgt ccaggtcccg tgcgtgaagc caccggcacc gcccgcgtgg
     3001 ataatgcaac cggcccccga ctggcagaca ccgctgaacg ggattatttc accctcagag
     3061 agaggctgat cactatgcaa aaacaactgg aaggaaccca gaagtatatt aatgagcagt
     3121 gcagatagag ttgcccatat cgatgggcaa ctcatgcaat tattgtgagc aatacacccg
     3181 cgcttccagc ggagtataaa tgcctaaagt aataaaaccg agcaatccat ttacgaatgt
     3241 ttgctgggtt tctgttttaa caacattttc tgtgccgcca caaattttgg ctgcatcgac
     3301 agttttcttc tgcccaattc cagaaacgaa gaaatgatgg gtgatggttt cctttggtgc
     3361 tactgctgtc tgtttgtttt gaacagtaaa cgtctgttga gcacatcctg taataagcag
     3421 ggccagcgca gtagcgagta gcattttttt catggtgtta ttcccgatgc tttttgaagt
     3481 tcgcagaatc gtatgtgtag aaaattaaac aaaccctaaa caatgagttg aaatttcata
     3541 ttgttaatat ttattaatgt atgtcaggtg cgatgaatcg tcattgtatt cccggattaa
     3601 ctatgtccac agccctgacg gggaacttct ctgcgggagt gtgcggggat aatcaaaaac
     3661 gatgcacacc gggttttctc atttttcacg agatgggagc gatttcccgc gaagccgcct
     3721 gtccggtgcg gtggtggaag aaaccggata aaacaaccgc attgtgcaaa tatcgatcaa
     3781 atatggtgct gctgtgtgaa atctgaaaaa tcacagcggt cattatgcat cagtttttaa
     3841 cacaggacgt cagaacgtga catggcaaag ctggactgga aaaagctgga gcaggcattc
     3901 cgacgcgaac atgccgaaac gggaataaca ttactggact ggtgccggaa gaaaaagatt
     3961 aattacaaca ccgccagaac ccgtataaaa atgggcaaaa tcgatcatga aattgatcat
     4021 aaaaccgatc atgaaatcga tcatgacatc tcagatgaag aaccctgcaa tgacgcgggt
     4081 tccggcgatg aaaaatgcgc aaaaaactct gaaaaaaact gcgcaaattc ggcagaaacg
     4141 aaacggattc gtggttcccg acttttacca ccttcaaacg ctttttctca gcgaaacacc
     4201 cacgccgtaa gacaccgtgg atatgcgaag tatcttgagg cagataacct catggatgat
     4261 gcgtccgaca tggtgctgtt cgatgaactg gtgttcaccc gggcccgcgc actttcagta
     4321 actaaggcac ttaaagggat gttcgccgac ctggaagagg caactgacgt ggaaacccgt
     4381 gttgctcttt acgacaaaat actcaaagct gaacaggccc ttgaccggaa tattgcccgt
     4441 atcgagtcaa ttgaacgctc attgctgacg ctggacgtcc tggctgagac agcaccaaaa
     4501 cttcgtgctg accgggaaag aatcaacgcc gccagagata aactcagagc tgaaaccgat
     4561 attctgacca accagcgtcg gggcgttgtt acgcctgtca gtgacatcgt gtcatcgctg
     4621 catgaaatga gtaattcggg gagactggat gacattccgg aagaatgaac cgcgatgtga
     4681 tgagccgtca gaaatgaccg aggctgaaca acgtctgttc atcatgacta aactgagcaa
     4741 tccctggtgg cggctcaatc atctctacaa aatacagaac gaaaaaggtg aactggtcac
     4801 cttcagaatg cgaccggcgc agcgccagtt gttccggagc atgcacaata aaaatattat
     4861 cctgaaagcg cgccagctgg gattttccac agccattgat atttatcttc tcgaccaggc
     4921 attattcatt ccgcatctca aatgcgggat cgtcgctcag gataaacagg ctgccagtga
     4981 aattttccgc acaaaaattg ctgtaccgtt tgatcatctc cctgactggc tgagagcctc
     5041 attcaccatc gttgaacgtc gtagcggtgc cagcggtggc tatatcctgt ttggtcacgg
     5101 ctcgagtatc caggtggcaa cctcattccg ttcaggtacg gtgcagcgcc tgcatatctc
     5161 agagcacggc aaaatttgcg cgaaatatcc ggctaaggcg aaagaactgc gaaccggtac
     5221 gcttaatgcc gtctctgatg aatgcattat ttttgatgag tccactgctg aaggcgtggg
     5281 tggtgatttt tacgagatga gtaaccgagc acaggagatc actgcatcag gcttattgct
     5341 gacggcacag gattataaat tccattttta cgcctggtgg caggatccta aatacagcgc
     5401 cagagtgccg gaaagcgggc tgaagctgtc acgggaaaaa atgacgtatt tttctgcggt
     5461 tgagaaggca atgaacatca cgcttactga tgaacagaag cagtggtaca tcaataagga
     5521 aactgaacag cgtgaggaaa tgaagcagga gtttccctca acgccacagg aggcgtttct
     5581 gacgtccgga cgacgtgtgt tcagtgccga aagtacgttg caggcagaat cattctgttc
     5641 gccaccgatg attgtttatg acattgaacc tgttacagga gcgaagacta aagctcagtc
     5701 tctgcgtgaa ggaaataaaa acgagttgca gcggacgctg atgaattatc tgctggtatg
     5761 ggaactgccg gatccggatg aagagtatgt ttgtggggca gatactgccg aagggctgga
     5821 gcacggagac cgctcatcgc tggatgttgt caaacgcagt aatggcgagc aggtggctca
     5881 ctggttcggg catctcgatg ctgaactttt tgctcatctc atttcgcagg tctgtcgtat
     5941 gtataacaac gcgtttgtgg ggccggagcg taataatcac ggacatgcag ttatcctgaa
     6001 actccgggaa ctctatccga cacgttatat ctacaacgaa cagcatcttg accaggcata
     6061 tgacgacgat acgccccgcc ttggctggct gacaacccgt cagagcaaac ctgttctgac
     6121 cgaaggaatg aaaacgcttc tgaataatgg aatatcaggg atccgctggt caggcacatt
     6181 atcggaaatg aacacctacg tttatgacgc gaaaggctcc atgaatgcac aggaaggctg
     6241 ctttgatgat cagctcatga gctacatgat tgcccaggag atgcgcgcca gaatgccggt
     6301 gagggtaaaa cagaaaacgg ataaacgcag aaccacacac tggatggctc actgatgaaa
     6361 aatgaaacta acaccatggc gacgaaaaac gacaatggag ccacgccgcg tttttctcag
     6421 cgccagttac aggcgctttg ttctgatatt gacagccagc ctaaatggcg tgatgccgca
     6481 aacaaggcct gtgcgtatta cgatggcgat cagttgccac cggaagttct tcaggtactg
     6541 aaagatcgcg gtcagccgat gactatccat aacctcatcg cgcctaccgt cgatggcgtt
     6601 ctgggaatgg aggccaaaac acggactgat ctggtggtga tgtcagacga gccagatgat
     6661 gaaactgaaa aactggctga agctattaat gctgaatttg ccgatgcatg ccgccttggc
     6721 aatatgaata aagcccgctc tgatgcctat gcggaacaaa tcaaggcggg cctcagttgg
     6781 gtggaggtca gacgaaacag cgatccgttc gggcctgaat ttaaggtgtc tactgtcagc
     6841 cggaatgagg ttttctggga ctggctgagc cgggaggctg atttaagtga ctgccgatgg
     6901 ctgatgcgtc gccgctggat ggataccgat gaggcaaaag ctacattccc gggaatggct
     6961 caggttatcg attatgccat tgatgactgg cgtggttttg tcgataccac ggttactgaa
     7021 ggccagccca gtccgttgat gagtgcatgg gaagagtatc agtcatggga tcgacagcag
     7081 aacgaatggc ttcagcgtga acgccgtcgt gtgctgcttc aggtggttta ttaccgtaca
     7141 ttcgagcgtc ttccggtgat tgaactcagt aatggacggg tggtggcctt tgataaaaat
     7201 aatctgatgc aggcggtagc tgtggcatcc gggcgggttc aggtgaaagt cgggcgggta
     7261 agccgtattc gtgaagcctg gtttgtcggg ccacacttta ttgtggatcg cccctgtagt
     7321 gctccgcagg ggatgtttcc gctggttcct ttctggggat accgaaagga taaaaccggg
     7381 gagccatacg ggctaatttc ccgcgccatt ccggcacagg atgaggtgaa ttttcgtcgt
     7441 atcaagctga cctggttgct tcaggccaaa cgcgtgatta tggacgagga tgccacccag
     7501 ttgtcagaca acgacctgat ggagcagatc gaacgtccgg atggcattat taaactgaat
     7561 ccggtccgaa aaaatcagaa aagtgtcgca gatgtttttc gggttgagca ggattttcag
     7621 gttgccagcc agcagtttca ggtcatgcag gaatcggaaa aacttatcca ggataccatg
     7681 ggagtgtatt ccgcatttct cgggcaggat tcaggtgcga cgtcaggcgt ggctatcagt
     7741 aacctggtgg agcagggggc cacaaccctt gcggaaatca acgataacta ccagtttgcc
     7801 tgccagcagg tgggaagact gttgctggct tatcttctcg atgacctgaa aaaacgccgt
     7861 aatcatgcag tggtgattaa tcgcgatgat cgccagcgtc gccagaccat tgtcctcaat
     7921 gctgaaggtg ataatggtga actgaccaat gatatttcaa ggttaaatac acatattgcg
     7981 ctggcgcctg ttcagcagac accggcgttt aaggcacagc ttgcacagag aatgtcagag
     8041 gttattcagg ggctgccgcc tcaggtgcag gctgttgtgc tcgacctgtg ggttaatctt
     8101 ctggatgtgc cgcagaaaca ggagtttgtt gagcgtattc gtgctgcgct ggggacgcca
     8161 aaatcaccgg atgaaatgac gccggaagaa caggaagtag cggcacaaca acaggcactt
     8221 cagcaacaac aggcagaact ccagatgcgc gagatggctg gcagagtggc aaaactggaa
     8281 gctgacgccg ccagggcaca tgcagctgca caacgggata atgccagtgc acagcgggaa
     8341 gtcgccctga cacaggggca gcgttatgtg gatgcgctta accaggcaca tacggcagaa
     8401 atcattaccg gcgtacagaa tatggaacag gagcaggacg ttcttcagca acagatgctg
     8461 tatacgttac aacagcggat gaatgaaatg tcgctctgaa aactctggct tcaactgaac
     8521 cccgtcatcg tacggggttt tttgtttccg gaggtaagcg ttccgggagc ggtgcgctta
     8581 ttcgcggggg cagcgataag ccttatttac tcaaccattc ggatctgtcc gataaacaga
     8641 ccatgcggag ttatttatgg attttgaatt tacgggtgaa gaaaccccgg aacaactgga
     8701 aaaaatgctg gaaggacttg gggatgtgga tattgacagt cacgcacagg acgtcgtgac
     8761 ggaagatacc acggaaaaac atgcggatga ggaagcacag actcagacgg gcgataacaa
     8821 tgtggcaccg acgccggatg ccagtgtgga gcagacgcag gacgtgaagg agccggaagc
     8881 gaagggggtg ctcacccgcg acggtaaaca cgtcattccc tatgaagtcc ttgaggctga
     8941 acgttccggt aagcaacggg ccgaacagga agccgcactt cttcgtgggc agatagctga
     9001 agaaaaacgc agggtggaac tgctgacgtc tcagatccac caggccggta tgaagcccac
     9061 accgttaccg gaaaacgaaa aaatttctga tgagcagatt gcccgtatca gggagatgta
     9121 tccggaaatt ggtgacgcgg tggcttcgct catccgtaaa aataactatc tccagtcccg
     9181 tgttcagcaa tcagcacagc aggcagaagg taatggtggt gaggatttat caccggttct
     9241 tgatgcgatg aatgccgtgc cggtgctgaa aacgtggcag gagtccgatc cagatcgctt
     9301 ctcggttgct gtatccatcg acgggaagct ccagaatgac cccgcatgga aagacaaaac
     9361 gctcactgaa cgtttcgctg aagtggcccg tcgtacgcag gttgctttcg gtgaagtcag
     9421 tgagtcgtct gctgacaaca aggcagacaa aacggatatc cggaaaacgg cggaagagaa
     9481 agtgaagacc gctgaacagg agcaggcagt acctgcttcc ccgtcagatt taggcaccac
     9541 ggcttccgtc ggaaccggtg ataattttga acggttactt ggcgcttctc attcagaggc
     9601 agaggcgatt atgcgcggta tgacgaatgc tgaaatagac gcgcttctgg agaagctcgg
     9661 gtaacttact gaaggagaac tgaagtaatg acgactgtaa catcagccca ggcgaataag
     9721 ctgtatcagg tggcgctttt taccgctgcc aaccgcaacc gctcgatggt aaatatcctc
     9781 actgaacagc aggaagcgcc aaaagcggtt tcgccggaca agaaaagcac gaagcagacc
     9841 agcgcgggtg cgccggttgt ccgtatcaca gaccttaaca aacaggccgg tgatgaagtg
     9901 accttcagca tcatgcacaa actctcaaaa cgtccgacga tgggagatga gcgtgttgaa
     9961 ggtcgtggtg aggatctcag ccatgctgac ttctccctga aaatcaatca gggacgtcac
    10021 ctggtggatg caggcggacg tatgagtcag cagcgcacga agtttaacct ggcatcctca
    10081 gccagaacgc ttctggggac gtactttaat gacctgcagg accagtgtgc gatagtgcat
    10141 cttgctggag ctcgtggtga ttttgttgct gacgacacta ttctgccgac agcggagcac
    10201 cctgaattca aaaaaatcat gatcaacgat gtactgcctc cgacacatga ccgtcacttt
    10261 tttggcggtg atgcgacaag ctttgagcag attgaagcgg cagatatttt ttctattggc
    10321 ctggtggaca atctctccct gttcattgac gaaatggcgc atccgttaca gccggttcgt
    10381 ctgtccggtg atgaacttca cggagaagat ccatattacg tcctgtacgt cacgccgcgt
    10441 cagtggaatg actggtacac ctcgacgtcc ggtaaggact ggaaccagat gatggttcgt
    10501 gccgtgaacc gtgcaaaagg ttttaatcat ccgctgttca aaggtgaatg tgcgatgtgg
    10561 cgcaatatcc tggttcgtaa gtatgcgggt atgccgatcc gtttctatca ggggtcaaag
    10621 gttctggtat cagagaataa cctgacggca accacgaaag aggtcgctgc tgcaaccaat
    10681 attgaccgcg ccatgttact gggggctcag gcgctggcaa atgcttacgg tcagaaggcg
    10741 ggcggtcact tcaacatggt tgagaagaaa acggatatgg ataaccgtac tgagatagca
    10801 atcagctgga tcaacggtct gaaaaaaatc cgtttccccg agaagagcgg caagatgcag
    10861 gatcacggcg tgattgccgt tgatacagca gtgaagctct gattttttcc tttccctatg
    10921 ccgggttttc gcccggcttt ttcaggagtc attaattatg gcaaagacta tccttgcccc
    10981 gtcactgagt gaacgggtct atacgggtac gcacggtaat gagtcggtgg cagaaggcgt
    11041 atttacggtg aatgctgcgg aagcggacag tgttattcat cttctctcac tgccagtggg
    11101 catccgtatc aactcactcc aactggtttc aacgggtggt ctgggtactg caaccgtcag
    11161 cattaagtcc ggtgagcatg ctctcatcga taacagcgaa gctgtttctg caaaatttgc
    11221 cagatatgtg ccagtggagc cgtacaccac acagcgtgac ggggagctgg ttactgtcac
    11281 cattaagact gccgctgcaa ccggcaccct gaatgttctg ctgcgttata ccgtggtggg
    11341 atactgatta aaaccttccg gcccgcgtca tgcgggcttt ttatccgggg aattatatga
    11401 gtgagaaaat tgccgttgtc tatatcggcc caaaacccgt gaaaaaggac accattaccg
    11461 gaagtcgcac gctgttccca cgtcttgagc cggtgcatgt tgacagcgcg atggcctggc
    11521 aactgctggg gtttccggat gtctgggttc gtcatgaaga gcttgatgat gttctgaaaa
    11581 agcaacaaca gaatgagcag ttgcggcagg cacagcaggc gcaggaaaga gtgcttgctg
    11641 cgctggcaga agcggagaac agttttgttg tttctgttaa cgggcaggag gtggatttaa
    11701 gtaagctcac ctcagcacgg ctggcgacgc tgtgtgaggc agaagagctg gatattcaca
    11761 aagacccgaa agaaacggct gaggcattcc gtatccgggt gcgtgaggca tttcgccgtc
    11821 gtgttgcgga gactgaacag catggcggaa ctgagtgatt ttttaccgta tgtccgtcgt
    11881 catatcagcg gtccactgaa cattatgatg acggatgctc tgtcaatggc tgccgtggca
    11941 ttcagccgcc agtcgttggt gtgccgtcgg gaggttactg ttgtaccagt agcaggaaaa
    12001 gaaatcgtgc ttccgtatga caaagatgat gaggagtgcg ttcatatcat ccgtatctct
    12061 gacgataatc atgagctttt tgtcggtcgg gatgtggata tcagctccgg acgctccctg
    12121 cgatttgcct gttctcccgg tgaggtgagc gtgctttatg ccgtcgctcc gaaagccgga
    12181 cgcagccaga taccggatga actcctcaca tggcctgaag aagtggctgc gggggcactt
    12241 gagcggttgt tcatgcagac tggtgtttca tggtcagatc cgttacgcgc acagtatttt
    12301 tctgtgcagt tttctgaggg gatccgtcgg gcatatcgtc atacactggc gacaagcccg
    12361 tactcttcat accgcaaccc tgtacgcagg cagaggtttt tctgatgacg acgattacag
    12421 aaatcatcgg acgtgtgaat acacaactgg ttgacccgat gatggttcgc tggcccctgc
    12481 aggaattgtg cgattattac aatgatgctg tgagggcagt gattctggcg agaccggatg
    12541 ctggcgcaag cctggaaaca ataagttgtg ttcctggcgc ccgtcaggtt ttgcccgatg
    12601 gtgtaataca acttcttgac gtgatatgcc tcagtgacgg tagtgcagtc agaccattat
    12661 cccgggaggt gctggatgcg cagtatcctg agtggcccac aatgaagggt attcctgaat
    12721 gttttatcag caacgacctg tccccgcgcg tattctggct gtttcctgct cctgacaaag
    12781 agataagtat tgatgcagtg gtaagccgga taccggaggc agtgtatgtt ctgacgcagg
    12841 acgatgatac gccagttcca ctggaagagg cttatgttaa cccactggtg gagtggatgt
    12901 tgtttcgcgc tttcagtaag gatgctgccg gtggcgcaga atcggggctg gctgcgcagc
    12961 attatcagag ttttgttgag caacttggga tcaaacaggg ggcagacagt gcattgtctg
    13021 cccgtaaaaa agtgtttaac ggaggtggag tgtgagtgtt gttgtttcgg ggacgctgaa
    13081 atctcctgat ggtgaggcga tatcaggagc aaatattacc ctgacggcgc tgacagtttc
    13141 accggatgcg ctcagcggca ccagtgcgtc ggcagtgacc cgtgaaggtg gatattacgg
    13201 aatgacgatg gatccggggg agtatgcggt ttcggtgacg gtgaaaggga agactgctgt
    13261 ctacggacgt gtgcgtattg aggggaccga aagtacggtg acgctcaata tgctgttacg
    13321 ccgcagtctt gttgaggtta gcatacccgg agaactgctg acagatttcc ggcagataca
    13381 gaataatgtg gctgatgacc ttgccactat tcgtcgcctg aatgaagaca cggcgacaaa
    13441 aaacactcag gccacacagt caaaagaaag tgcagcagcc agtgcgaaga gtgcatctga
    13501 cagtgcaaag acggcaacca gcagggcggc tgaagccgga caaaaagcga ctgatgccac
    13561 tgaggctgcg acccgtgcag tcacagcagc ggggaatgca gaggaaagct cgacccgtgc
    13621 cggagagtct gaaaaagccg ccggagctga tgcagaaaaa gccagacagc atgctgaaaa
    13681 ggccaggctg gcgcaggaga gcgccggaga gatccttaag cgggcagagg ctgccactgt
    13741 cagtgctgaa gaggccagac gtatggctga gaatgcacgg gggccccggg ggcctcaggg
    13801 agaaactggt ccgaaggggg atgtcggtcc taaaggcgaa acaggtccag tgggccctca
    13861 agggcccgca gggccgaaag gtgagcgtgg tgacgttggt gctcaggggg ctgtagggcc
    13921 tgctggtccg cgtggtgaga agggcgaaca gggggagcga ggaccgcagg gaataccagg
    13981 cctgaagggg gatac
//
LOCUS       DAZUFB010000125        13900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZUFB010000125 DAZUFB010000000
VERSION     DAZUFB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.125"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..224)
                     /locus_tag="ACHY3M_004194"
     CDS             complement(<1..224)
                     /locus_tag="ACHY3M_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319004.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASKVFHAFIDLV"
     gene            626..1339
                     /gene="aphA"
                     /locus_tag="ACHY3M_004195"
     CDS             626..1339
                     /gene="aphA"
                     /locus_tag="ACHY3M_004195"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFS4319005.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1450..1866
                     /gene="yjbQ"
                     /locus_tag="ACHY3M_004196"
     CDS             1450..1866
                     /gene="yjbQ"
                     /locus_tag="ACHY3M_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFS4319006.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1870..2226
                     /gene="yjbR"
                     /locus_tag="ACHY3M_004197"
     CDS             1870..2226
                     /gene="yjbR"
                     /locus_tag="ACHY3M_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4319007.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2261..5083)
                     /gene="uvrA"
                     /locus_tag="ACHY3M_004198"
     CDS             complement(2261..5083)
                     /gene="uvrA"
                     /locus_tag="ACHY3M_004198"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4319008.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5337..5873
                     /gene="ssb1"
                     /locus_tag="ACHY3M_004199"
     CDS             5337..5873
                     /gene="ssb1"
                     /locus_tag="ACHY3M_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFS4319009.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5972..6253)
                     /gene="yjcB"
                     /locus_tag="ACHY3M_004200"
     CDS             complement(5972..6253)
                     /gene="yjcB"
                     /locus_tag="ACHY3M_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFS4319010.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            6683..8269
                     /gene="pdeC"
                     /locus_tag="ACHY3M_004201"
     CDS             6683..8269
                     /gene="pdeC"
                     /locus_tag="ACHY3M_004201"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFS4319011.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8272..8595)
                     /gene="soxS"
                     /locus_tag="ACHY3M_004202"
     CDS             complement(8272..8595)
                     /gene="soxS"
                     /locus_tag="ACHY3M_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFS4319012.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8681..9145
                     /gene="soxR"
                     /locus_tag="ACHY3M_004203"
     CDS             8681..9145
                     /gene="soxR"
                     /locus_tag="ACHY3M_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFS4319013.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9691..11040
                     /gene="ghxP"
                     /locus_tag="ACHY3M_004204"
                     /gene_synonym="yjcD"
     CDS             9691..11040
                     /gene="ghxP"
                     /locus_tag="ACHY3M_004204"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFS4319014.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11192..12841
                     /gene="yjcE"
                     /locus_tag="ACHY3M_004205"
     CDS             11192..12841
                     /gene="yjcE"
                     /locus_tag="ACHY3M_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFS4319015.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12995..13900)
                     /locus_tag="ACHY3M_004206"
                     /pseudo
     CDS             complement(12995..13900)
                     /locus_tag="ACHY3M_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         3330 a         3332 c         3568 g         3670 t
ORIGIN      
        1 actaaatcaa taaatgcgtg aaaaacttta cttgcaatag aacttgatac ttcttctcca
       61 ggtctacaaa aatcttttgc aatttttaac tgtgtttcta tgtataaaaa gaaatctttg
      121 gcataagaaa aatctttggc ataagagata tctttattat ttagtgcatc tagctcaaca
      181 ggtaaagatg tcgttttgtt gctattcaca tatgatatat tcatatcatc atctccatcg
      241 cgagtaataa aattaatcac cattgtaggg tagggggctg gtcaatcaga aatcatcttt
      301 ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg tacagcagat
      361 aaatgttcta tcaaatttcg ctcatttgcc gaggattcat cataataaac gtaaaattaa
      421 tgtatcctta catcgagtaa taaacatttt ttatacaaaa aaagacagga acgtatttac
      481 tgggttaaat ataatcatcc tgcttttcat cacaaaaacc gcagataatc cttcctttcc
      541 ccggcagctg gcgttatggt cagatggttt ttgcaacaaa tctcacaata aaaagtttca
      601 acatactgac tatttaggga aaaatatgcg caagatcaca caggcaatca gtgccgtttg
      661 cttattgttc gctctaaaca gttccgctgt tgccctggcc tcatctcctt caccgcttaa
      721 ccctgggact aacgttgcca ggcttgctga acaggcaccc attcattggg tttcggtcgc
      781 acaaattgaa aatagcctcg cagggcgtcc gccaatggcg gtggggtttg atatcgatga
      841 cacggtactt ttttccagtc cgggcttctg gcgcggcaaa aaaaccttct cgccagaaag
      901 cgaagattat ctgaaaaatc ctgtgttctg ggaaaaaatg aacaatggct gggatgaatt
      961 cagcattcca aaagaggtcg ctcgccagct gattgatatg catgtacgcc gcggtgacgc
     1021 gatcttcttt gtgactggtc gtagtccgac gaaaacagaa acggtttcaa aaacgctggc
     1081 ggataatttt catattcctg ccaccaacat gaatccggtg atctttgcgg gcgataaacc
     1141 agggcaaaat acaaaatcgc aatggctgca ggataaaaat atccgaattt tttatggcga
     1201 ttctgataat gatattaccg ccgcacgcga tgtcggcgct cgtggtatcc gcattctgcg
     1261 cgcctccaac tctacctaca aacccttgcc acaagcgggt gcgtttggtg aagaggtgat
     1321 cgtcaattca gaatactgac agagcgggag agcgtgacgc tctcccgaat gctgtttttt
     1381 taatcacacc tttatccttt cgctgtcttg ctgcaaactg attaagagag ttttatcaag
     1441 gagcagcaca tgtggtatca aaagacgctc acgcttagcg ccaaatctcg tgggtttcat
     1501 ctggtaacgg atgaaattct gaatcagctg gctgatatgc cgcgcgttaa catcggctta
     1561 ctgcatctgt tgctgcaaca tacctccgcc tctctgacac ttaatgagaa ctgcgatccc
     1621 accgtacgcc acgacatgga gcgttttttc ctccgcaccg ttcccgacaa cggaaattat
     1681 gagcatgact atgagggagc agacgatatg ccttctcata tcaaatcctc aatgctggga
     1741 acatcgcttg tattgccggt acataaaggg cgtattcaga ccggcacctg gcaaggcatc
     1801 tggctggggg aacatcgcat ccacggcgga tcgcgtcgca tcatcgcgac actacaaggg
     1861 gagtaaaaaa tgaccatttc ggagttgcta caatattgca tggcaaaacc aggcgcagaa
     1921 cagagcgtgc ataacgactg gaaagcgacg cagatcaaag tggaagatgt actgtttgcg
     1981 atggtgaaag aagtagaaaa tcgcccagct gtttcgctga aaaccagccc ggagctggcg
     2041 gagctgctac gtcagcagca cagcgatgtg cgtcctagcc gccatctgaa taaagcgcac
     2101 tggagcaccg tgtatctcga cggttcgctg ccggattcgc aaatctatta tctggtggat
     2161 gcgtcttatc agcaggcagt gaatttacta ccggaagaaa agcgtaaatt gctggtacaa
     2221 ctctgaaagg aaaaggccgc tcagaaagcg gccttaacga ttacagcatc ggcttgagga
     2281 agcgtgccgt atgcgaagct tcgcactccg cgacggtttc tggcgtaccg gagacgagga
     2341 tttcgccgcc gccactgccg ccttctggtc ccaggtcgac aatccagtca gcggttttga
     2401 tcacgtcgag attgtgctca atcaccacaa tggtgttgcc ctgatcgcgc agtttatgca
     2461 gtacgtcgag cagttgctga atatcggcga agtgcagacc ggtggtcggc tcgtcgagaa
     2521 tatacagcgt ctgcccggtg ccgcgttttg acagctcacg cgccagcttc acgcgctggg
     2581 cttcaccacc agaaagtgtg gttgcggact gccccaggcg aatgtacgtc aggccaacgt
     2641 ccatcaacgt ttgcagctta cgcgccagtg caggtacggc atcaaagaac tcacgcgcct
     2701 cttcgatggt catatccagc acttcgtgga tggttttgcc tttgtactta atttccagcg
     2761 tttcacggtt atagcgttta cctttacact gatcgcacgg cacgtagata tccggcagga
     2821 agtgcatctc tactttgatc acaccgtcgc cctgacaggc ttcgcagcgc ccgccacgga
     2881 cgttaaagct gaaacgtcct ggcgtataac cacgcgcacg ggattccggt acgcccgcaa
     2941 acagttcgcg cacaggcgta aacacgccgg tataggtcgc cgggttagaa cgtggagtac
     3001 gaccaattgg gctttggtcg atatcgatca ctttatcgaa atgctccagc ccctgaatat
     3061 cgcgatacgg tgctggttcg gcgatggtcg ccccattcaa ctggcgttgg gcaatcggga
     3121 acagtgtgtc gttaatcagc gtcgatttac cggaacctga aaccccggtg atgcaggtaa
     3181 acagacccac cggcagcgtc agcgtcacgt ccttcaggtt gttgccgcgt gcgcctgtca
     3241 gcttcagcac tttttccgga ttcgccggaa cgcgtttctt cggcacttca atcttgcgtt
     3301 tgccgctcat gtactgcccg gtcaacgact ccggcaccgc cataatcgct tccagcggac
     3361 cttctgcgac cacttcaccg ccgtgaacac ctgcgcccgg gccaatgtcg atcacatggt
     3421 cagcggcgcg aattgcgtct tcgtcgtgct ccaccacaat cacggtatta ccgagatcgc
     3481 gcagatggat aagcgtaccc aacaggcgct cgttatcgcg ctggtgcagg ccgatagatg
     3541 gctcgtccag cacgtacata acgccaacca ggcccgcacc aatctggctc gccagacgga
     3601 tacgctgggc ttcaccgccg gaaagcgttt ctgccgagcg ggaaagcgtc aggtaattca
     3661 ggccgacgtt aacgaggaat ttcaaacgat cgccgatctc tttaaggatt ttttccgcaa
     3721 tcttcgcccg ttgaccagcg agtttgagat tgttgaagaa ttccatcgca tgaccgatgc
     3781 tcatgtcgga gatagcaggc agcggcgtat tctcgacata aacgtggcgc gcttcccgac
     3841 gcagacgcgt cccttcgcag ctggcgcatg ggcgattgct gataaacttg gctaattctt
     3901 cacgtaccgc actggattct gtctctttat aacggcgctc catattgtgc agcacgcctt
     3961 cgaacggatg acgacggatg gaggtatcgc cacgatcgtt catgtatttg aattcaatgt
     4021 tttctttgcc agaaccgtac aacaccactt tatgcacgtt cgcgctcagg ctgccccacg
     4081 gtgcttcgac gtcgaactta tagtgatctg ccagcgattt cagcatctgg aagtaataga
     4141 agttgcggcg atcccagcca cggatcgcac cgccagccag tgacagctcg gggttttgga
     4201 tcacgcggtc aggatcgaaa tattgctgta cgccaaggcc gtcacaggtc gggcaggcac
     4261 ctgccgggtt gttaaacgaa aacagtcgcg gctccagttc acgcatactg tagccgcaaa
     4321 ttgggcaggc gaagttggcg gagaacagca gctcttccgc tttcgggtcg tccatatccg
     4381 ccactaccgc tgtaccaccg gaaagctcca gcgcggtttc aaacgactcc gcaagacgtt
     4441 gggtaagatc gtcacgcacc ttgaagcgat caaccaccac ttcaatggta tgtttctttt
     4501 gcagttccag tttcggcgga tcggaaagat cgcagacttc gccatcaata cgagcacgga
     4561 tgtaaccctg gcttgccagg ttctccagcg ttttggtgtg ttcgcctttg cgctctttaa
     4621 tgattggcgc gagcagcatc agacgcttgc cttccggctg cgacagcacg ttatccacca
     4681 tctggctgac ggtttgcgcc gccagcggga cgtcgtggtc cggacagcgc ggttcgccga
     4741 cgcgggcgaa caacaaacgc aaatagtcgt ggatttcggt gattgtcccc accgtagaac
     4801 gcgggttatg agacgtcgat ttctgctcaa ttgagatggc aggagaaagc ccctcaatat
     4861 gatcgacgtc cggcttttcc atcagtgaca gaaactgccg cgcgtaggcg gaaagggatt
     4921 caacgtaacg gcgctgccct tcggcatata aggtgtcgaa agcgagcgag gatttgccag
     4981 aacccgaaag cccggtcacg acaatgagct tgtcgcgggg gataacgagg ttgatgtttt
     5041 tgagattatg ggtgcgggcg ccccgaactt cgatcttatc cattcacctt tcccggatta
     5101 aacgcttttt tgcccggtgg catggtgcta ccggcgatca caaacggtta attatgacac
     5161 aaattgacct gaatgaatat acagtattgg aatgcattac ccggagtgtt gtgtaacaat
     5221 gtctggccag gtttgtttcc cggaaccgag gtcacaacat agtaaaagcg ctattggtaa
     5281 tggtacaatc gcgcgtttac acttattcag aacgattttt ttcaggagac acgaacatgg
     5341 ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac ccggaagtac
     5401 gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc gaatcctggc
     5461 gtgataaagc gaccggcgag atgaaagaac agactgaatg gcaccgcgtt gtgctgttcg
     5521 gcaaactggc agaagtggcg agcgaatatc tgcgtaaagg ttctcaggtt tatatcgaag
     5581 gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac accacagaag
     5641 tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt ggtggcgctc
     5701 cggcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag ccacagcagc
     5761 cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag tccgctccgg
     5821 cagcgccgtc taacgagccg ccgatggact ttgatgatga cattccgttc tgatttgtca
     5881 ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc agccataaaa
     5941 aagaagcctc cgttatggag gcttctacgt atcaggtcaa aatcattggc cattgtggcg
     6001 gtgtctgatt catctcttct atcatcacca actcgatgtt gtgccagata gcggtgatgt
     6061 ccgccattgt gatgccaagc aaccccattg cgaaccaaca ggcggaaaca acgcccagac
     6121 cgctgctgat caccgcaagc ccgacataat cagaccgacg aaaacggatg ttgagtgtgc
     6181 tggccagaaa catcattacg gcactaagaa gttgccagcg aagaagaacc acgccagtgg
     6241 tgagggtagc catcaaacaa ttcctctgaa aagagccgat gccctggaca gcgcggcgtt
     6301 gttcacggga ggcgtggtac actctggcta tcgcggggct tgcagaacac aaaaatgaaa
     6361 cactctgttt gtttcattaa ttttgtgaac tatatcacaa ttgattgttt gttagccata
     6421 ttaggccgtg acttttattg ctgtacagat tatgtggttt ttcagtggca ttaagggcat
     6481 atcttcccgc cgcctctgca ttcctgtagg aaattaattt tgaatatcaa tgaattattt
     6541 tcatccaggt gacgattaga aaggtatcaa tttcaaatca ggcaaaagtg ctatttatac
     6601 cgtaagattt atctaaagac gtcggtaccc agggttttca ccttgcaatg gccgggtata
     6661 aacaggcagg aaattgatag caatgagtca tcgtgcacga caccaattac tggcgttgcc
     6721 gggcattatc tttttagttc tctttcccat cattctttcg ctatggattg ccttcctttg
     6781 ggcaaaatca gaagtgaata atcagctccg aacctttgct caactggcac tggataaatc
     6841 cgagctggtc attcgccagg cagatttagt gagcgatgca gctgaacgct atcaggggca
     6901 agtttgcact ccagcccatc aaaagcgaat gttgaatatt attcgtggct atctttatat
     6961 taatgaattg atctatgccc gtgataacca ttttttatgc tcatcgctga tagcgcctgt
     7021 aaacggctat acgattgcac cggccgatta taagcgtgaa cctaacgttt ctatctatta
     7081 ttaccgcgat acgccttttt tctctggcta taaaatgacc tatatgcagc ggggaaatta
     7141 tgtggcggtt atcaaccctc tcttctggag tgaagtgatg tctgatgatc cgacattgca
     7201 atggggtgtg tatgatacgg tgacgaaaac ctttttctcg ttaagcaaag aggcctcggc
     7261 agcaacgttt tctccgctga ttcatttgaa ggatttaacc gtacaaagaa atggctattt
     7321 atatgcgaca gtttattcga caaaacgccc aattgcagcc attgttgcga cttcatatca
     7381 acgtcttata acccattttt ataatcatct tatttttgcg ttgcccgccg gtattttggg
     7441 gagtcttgtt ctgctattac tctggctacg tattcgacaa aactatttat ctcccaaacg
     7501 taaattgcaa cgcgccctcg aaaaacatca actttgtctt tattaccagc caataatcga
     7561 tatcaaaaca gaaaaatgta tcggcgctga agcgttgtta cgttggcttg gtgagcaggg
     7621 gcaaataatg aatccggcag agtttattcc gctggcagaa aaggagggga tgatagaaca
     7681 gataactgat tatgttattg ataatgtctt ccgtgatctg ggcgcgtact tggcaacaca
     7741 tgccgatcgc tatgtttcta ttaacctgtc ggcctccgat tttcatacgt cacggttgat
     7801 agcgcgaatc aatcagaaaa cagagcaata cgcggtgcgt ccgcagcaaa ttaaatttga
     7861 agtgactgaa catgcatttc ttgatgttga caaaatgacg ccgattattc tggctttccg
     7921 ccaggctggt tacgaagtgg caattgatga ttttggtatt ggttactcta acttgcataa
     7981 ccttaaatca ttgaatgtcg atattttgaa aatcgataaa tcgtttgttg aaacgctgac
     8041 cacccacaaa accagtcatt tgattgcgga acacatcatc gagctggcgc acagcctggg
     8101 gttaaaaacg atcgctgaag gcgtcgaaac tgaggagcag gttaactggc tgcgcaaacg
     8161 cggcgtgcgc tattgccagg gatggttttt tgcgaaggcg atgccgccgc aggtgtttat
     8221 gcaatggatg gagcaattac ccgcgcggga gttaacgcgc gggcaataaa actacaggcg
     8281 gtggcgataa tcgctgggag tgcgatcaaa ctgccgacgg aaaacgcggg agaaggtctg
     8341 ctgcgagaca taacccaggt ccattgcgat atcaaaaatc ggacgctcgg tggtgcgcaa
     8401 ctcaacggcg gccagtaaca ggcgacgttg gcgaatgtaa tcgccaagcg tctgatgcgt
     8461 caccgtgcgg aacattcgtt gcaagtacca ctttgaatag cctgattttt ttgcgactac
     8521 atcaatgtta agcggctggt caatatgctc gtcaatccat gcgataagat cctgaataat
     8581 tttctgatgg gacataaatc tgcctctttt cagtgttcag ttcgttaatt catctgttgg
     8641 ggagtataat tcctcaagtt aacttgaggt aaagcgattt atggaaaaga aattaccccg
     8701 cattaaagcg ctgctaaccc ccggcgaagt ggcgaaacgc agcggtgtgg cggtatcggc
     8761 gctgcatttc tatgaaagta aagggttgat taccagtatc cgtaacagcg gcaatcagcg
     8821 gcgatataaa cgtgatgtgt tgcgatatgt tgcaattatc aaaattgctc agcgtattgg
     8881 cattccgctg gcgaccattg gtgaagcgtt tggcgtgttg cccgaagggc atacgttaag
     8941 tgcgaaagag tggaaacagc tttcgtccca atggcgagaa gagttggatc ggcgcattca
     9001 taccttagta gcgctgcgtg acgaactgga cggatgtatt ggttgtggct gcctttcgcg
     9061 cagtgattgc ccgttgcgta acccgggcga ccgcttagga gaagaaggta ccggcgcacg
     9121 cttgctggaa gatgaacaaa actaaagcgc cacaagggcg ctttagtttg ttttccggtc
     9181 tttgtctttc tctctatccc gctggtacac aggagggttt cccccgacgt caacacaccc
     9241 cattcgagca cgtggtggag gttccggttg gtgttgatgc tttaattgta tgtcaccggc
     9301 atttcttcgc cagtgtaaaa gtatactttt tatccgcaat atttttgtca tctcagacga
     9361 ttttttatcg caatcctgaa cggtatacgg ctcgataacg ctgcaatctt gcgcaccgac
     9421 gataacgttt gcgcgtcaat tgcctggttt ttcatcgtca agacaataaa agagaaaaaa
     9481 gcagcaaact tcggttgaaa aagccgctat gatcgccgga taatcgtttg ctttttttac
     9541 cacccgtttt gtatgcgcgg agctaaacgt ttgctttttt gcgacgcagt aaattgtcgc
     9601 aaacctggag caggaagata acgtttcgct ggcaggggat tgtccgccac gcatcttgac
     9661 gaaaattaaa ctctcagggg atgttttctt atgtctacgc catcagcgcg taccggcggt
     9721 tcactcgacg cctggtttaa aatttcacaa cgtggaagca ctgtccgtca ggaagtggtt
     9781 gccgggttaa caacgtttct ggcgatggtc tactcggtca tcgtcgttcc aggtatgttg
     9841 ggtaaagcag gcttcccgcc tgcggcagtt ttcgttgcaa cctgtctggt tgccggactc
     9901 ggttctatcg tgatgggtct gtgggctaat ctgccgttgg cgattggttg cgccatctcc
     9961 ctgacggcgt ttaccgcatt cagcctggtg ctggggcaac atattagcgt acctgtcgcg
    10021 ctgggtgccg tgttcctgat gggggtgctg tttacggtaa tttctgccac gggtatccgt
    10081 agctggattt tgcgcaactt gccgcacggt gtggcgcacg gcacggggat cggtatcggt
    10141 ctgttcctgc tgctcattgc cgctaatggt gtcggtctgg tgattaaaaa cccgcttgat
    10201 ggtctgcccg ttgcgctggg tgatttcgcg accttcccgg tgattatgtc actggtaggt
    10261 ctggcggtga tcatcggcct ggaaaaactg aaagtccctg gtggcattct gctgaccatt
    10321 atcggtatct caattgtcgg tttgatcttc gatcctaacg tccatttctc cggcgttttc
    10381 gccatgcctt cattgagcga tgaaaacggc aattcactga ttggcagcct ggacattatg
    10441 ggcgcgctga atcctgtagt cctgccaagc gttctggcgc tggtgatgac ggcagtattt
    10501 gatgccaccg gaactatccg tgccgtcgcc ggccaggcga acctgctgga taaagatggg
    10561 cagatcatcg acggtgggaa agcactgacc actgactcca tgagcagcgt tttctctggc
    10621 ctggtgggtg ctgctccggc agcggtatac atcgagtctg cggcgggtac ggcggcgggc
    10681 ggtaaaaccg gtttgacggc tatcaccgtt ggcgtgctgt tcctgctgat tctgttcctc
    10741 tctccgctct cttacctcgt tccggggtat gcaacggctc cggcgctgat gtacgttggc
    10801 ctgctgatgc tgagcaacgt ggcgaaaatc gactttgctg attttgttga tgcgatggcg
    10861 ggtctggtta cggcggtatt catcgtgctg acctgtaaca tcgtaacagg catcatgatc
    10921 ggcttcgcga ctctggtgat tggtcgtctg gtttccggcg aatggcgcaa gttgaacatc
    10981 ggtacggtcg ttatcgccgt ggcgctggtg accttctatg cgggtggctg ggctatctaa
    11041 tctttcatcg gattctgaaa acgggtggca atggctgccc gtttttattt tctccgcaca
    11101 ttgtgtgagc ttttgcgata ttctgaaaaa atgagaattc aggcataacg tctttctcag
    11161 atcataaaaa aacatcgcaa acagggaacg catggaaatc ttcttcacca tactgataat
    11221 gaccctcgtg gtctcgctgt ccggggtggt cactcgtgtc atgccctttc agatcccgct
    11281 tccgcttatg caaatcgcca tcggtgcgct actggcgtgg ccgacgtttg gtttgcatgt
    11341 ggagtttgat cctgaactct ttttagtctt gtttatcccg ccgttgctgt tcgctgatgg
    11401 ctggaaaacg ccgacccgtg aatttctcga acatggtcga gagatttttg gcctcgcgct
    11461 ggcgctggtg gtggtcaccg tggtcggcat tggcttcctt atttactggg tggtgccggg
    11521 cattccgctg atccccgcct ttgcgctggc ggcagtgctt tctccgaccg atgctgtggc
    11581 gctctccggg attgttggcg aagggcgcat cccgaaaaaa atcatgggta ttttgcaggg
    11641 cgaagcgttg atgaatgacg cctccggcct ggtgtcgttg aagtttgccg tggcagtggc
    11701 gatggggacg atgatcttca ccgttggcgg tgcgacggtc gaatttatga aagtagccat
    11761 tggcggtatt ctcgccggct ttgtggtgag ctggctgtat ggtcgctcgc tgcgattcct
    11821 cagccgctgg ggcggtgatg aacccgcgac gcagattgtt ctgctgttct tgctgccatt
    11881 cgcttcttat ctgattgccg aacatattgg cgtttccggc atcctcgctg cggttgccgc
    11941 cgggatgacc atcacccgct ccggtgtgat gcgccgtgcg ccgctggcaa tgcgcctgcg
    12001 cgcaaacagc acctgggcga tgctggaatt tgtctttaac ggcatggtgt tcctgctgtt
    12061 aggtctgcag ctgccgggta ttctggagac gtcgctgatg gcggcagaaa tcgaccctaa
    12121 cgtcgaaatc tggatgctgt ttaccgatat tattctgata tatgcggcgc tgatgctggt
    12181 ccgtttcggc tggctgtgga cgatgaaaaa gttcagcaac cgcttcctga agaagaagcc
    12241 gatggagttt ggttcgtgga ccacacgaga aatcctgatc gcgtctttcg ccggggtgcg
    12301 tggggcgatc acgctggccg gtgtgctctc tatcccgctg ctcttgccgg atggtaacgt
    12361 cttcccggcg cgctatgagc tggtgttcct ggcggctggc gtcattctct tctcgctgtt
    12421 tgtcggcgtg gtgatgttgc ctattctgct acaacacatt gaagtcgcgg accattcgca
    12481 acaattgaaa gaagaacgta ttgcgcgagc ggcaacggca gaagtggcga ttgtggcgat
    12541 ccagaaaatg gaggagcgtc tggcggcgga taccgaagag aatatcgata accagctgct
    12601 tacggaggtc agttctcgcg tcattggtaa cctgcgtcgt cgcgccgatg gacgtaacga
    12661 cgttgaaagt tccgtgcagg aagagaacct tgagcgtcgc ttccgtctgg cggcattgcg
    12721 ttctgaacgt gctgaactgt accacctgcg cgccacgcgg gagatcagca acgaaacgct
    12781 gcaaaaatta ctgcacgatc tcgatttgct tgaagcgttg ctaattgagg aaaatcagta
    12841 agccgaaagc gacgtaacca ggcccgaggt aatctgaaaa aggtgcaatc ttttgcacct
    12901 ttaacaggtt agctcgggct aacattcatg attctaaaac aaataagtta tctctcttgt
    12961 aaagtcaatt cattggctgt tcttcgactt aacctcaatc tgcgtgttgc ataatatctg
    13021 cagtaatctt catagcgact gcattaatat tatcctcagt ttgtgacatg ccagtatgag
    13081 aggtacaatc ttcttcaaag aatttctgta gtgttaccag ggtatcacta tcaatgggga
    13141 tattttcctc cccacggaga atagaaatgc ccattccctg gacgctagcc tcatcaaaat
    13201 taacccgata gagacgggta ttaaacatac aggcttttgt taaaatagaa cagttgaaat
    13261 ggcagttgct taaattggca ttattaaaat taacattagc catattagag ccggtacata
    13321 taaccatttc gaggaatgta tttgttagcc acgtacaaaa gagtttgcta tcaattaagt
    13381 ttatattctg gaggattgct ttgttcagat tgacgcgaga cagatcagca tgttcaaagt
    13441 taattaaatc gagtttagag aaactaaggt cggctttaaa taaattaaca tatggcgcta
    13501 ttactttata aagatttacc tcagccatgt acgccatata aaaatttgaa tagtcaagaa
    13561 aggtattttt aatattggct ctattcattg ctgcattgta gaattgaaca tgcatagcgg
    13621 agcatgcatt tagagttccg tcggccattt ttatctgagc gaaatttgag tcatcgagaa
    13681 tacaattatc aaaaagtatt ttttccatta tggcgtaata aaaattcgtt ttatacatat
    13741 tacagtcctg gaaaatagcg ttttgtagtc agcaacctgc aaaattcgaa ttggataaat
    13801 cttcatcttt aatcacgata cctttcaggc taaggtgtga aaaatcgtaa ccaccattta
    13861 ctgtctgtgg aatttcgata gtattattat ggacggcgac
//
LOCUS       DAZUFB010000126        13252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZUFB010000126 DAZUFB010000000
VERSION     DAZUFB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.126"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..382
                     /locus_tag="ACHY3M_004207"
     CDS             <1..382
                     /locus_tag="ACHY3M_004207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS4319016.1"
                     /translation="SIISEGFDWNDGQVVDFFKRIIKQKRDLTRESGAWEQYEQLAKW
                     LIYFGSLLEIGGTKYENEYLDAVEYSMSNMSKEMRLGYSWHAYRAWETRWSTIIASNR
                     LIIKKRIEEIGVLEDANSIVKDIV"
     gene            complement(434..682)
                     /locus_tag="ACHY3M_004208"
     CDS             complement(434..682)
                     /locus_tag="ACHY3M_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HFS4319017.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(679..1119)
                     /locus_tag="ACHY3M_004209"
     CDS             complement(679..1119)
                     /locus_tag="ACHY3M_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4319018.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            complement(1153..7920)
                     /locus_tag="ACHY3M_004210"
     CDS             complement(1153..7920)
                     /locus_tag="ACHY3M_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS4319019.1"
                     /translation="MNKLSMGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAISTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDESTLESANVLWPTFISGKWFEKDGRRFVHGTQEKGFRGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVGEGEMRLINGVWQKYAGGRWIEA
                     DAGKELKIDAASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLV
                     DWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRF
                     GNPGRGPIAKLSGSGARAWFAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTL
                     RYLYSDLTRDPIQLDDFRLAFTGELPASDDELLNLLASTPGIAVSPYGGIVPFARATS
                     GDINEIVAPKQEFLATLPDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSV
                     ILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRY
                     EWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRY
                     DELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGT
                     GLGKTTTALALEAFNYENGRSTRTAYVVPKAVLENWYYEAKEFLSEEAFSNYLFVGLD
                     VLMDGDQIRQVPVLDENGKPVLGTDGTPVMRDALKLADEATITARMNAIPHSNYRAVV
                     FTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRWVLSEYSDT
                     GTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIK
                     NAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQKMGIYGPDDFVKFFGK
                     TRLETVQKISGEVEEKMALVGFENLDALRGIFHRWVTLKTAEDVKDTVEIPELDEHQQ
                     DAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVC
                     TDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEF
                     IQLQVPEAFEQEVNKRLARFGIDEQTVTHPVTPKYAKLIATLKEFFPEGKQIIFTDEK
                     TQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIA
                     KYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDI
                     HHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDI
                     LRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQ
                     NYIKAQHAAGEDVEVLTAELERRKAELEKTTADVAKFKQAVMAKAADNADWKARWGSV
                     HHTDRTLLAQYRASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAIN
                     KGILDVDPDLVNHASEFLIIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTF
                     KGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIK
                     ITDSYGVQTTESGYSIDFVGTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQ
                     VRLAEVFLTELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSE
                     LEVTNAIFGTHEFRASDYEITTAQFGTIGIYSNKAEIKQAMDAASARIAAEREANLNH
                     AVAALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYQMDAVKLPSAYGQLSYRM
                     TYNLVSMFSDLAILGLVDLNEVTPELLSMRKNHVEILQRINTVLAGRTDEEKQADADR
                     INLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAA
                     GEVFGLQEASGMKGVLFRAKDAIKAKFGARWLPAKAKNSDFPGNWWIIETKHNVADVL
                     AVIQQYA"
     gene            7996..9705
                     /locus_tag="ACHY3M_004211"
     CDS             7996..9705
                     /locus_tag="ACHY3M_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4319020.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            9698..10717
                     /locus_tag="ACHY3M_004212"
     CDS             9698..10717
                     /locus_tag="ACHY3M_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HFS4319021.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYSKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFK"
     gene            complement(11009..11566)
                     /locus_tag="ACHY3M_004213"
     CDS             complement(11009..11566)
                     /locus_tag="ACHY3M_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4319022.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            11736..12224
                     /locus_tag="ACHY3M_004214"
     CDS             11736..12224
                     /locus_tag="ACHY3M_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4319023.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSTNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFDDDI
                     PF"
     gene            12422..13210
                     /locus_tag="ACHY3M_004215"
     CDS             12422..13210
                     /locus_tag="ACHY3M_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319024.1"
                     /translation="MTAQNTKTIQYRLRNGQSVEVTVNNDGVPGETVSISDLAIEKTI
                     MCHLGFTEEVSKKHGVAIWSAMDTGMRRFITARTPGMTMMDLMQIAPLFECEPLDVFS
                     NPAICQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIK
                     SDLKRAILEASCRVVGLGKQSYVSFPAGPEGPAEILIMDADLLWQIQFLIGKSIIRDE
                     ELDQYITCTMTDEVKSVAIANARNLCRAALTELQENTTGEVESD"
BASE COUNT         3204 a         3479 c         3257 g         3312 t
ORIGIN      
        1 ctcgattata tctgagggtt tcgactggaa tgacggacaa gtagttgatt ttttcaagag
       61 aataataaaa caaaaaagag atttaacaag agagagtggt gcttgggagc aatacgagca
      121 gcttgcaaaa tggcttatat atttcggcag ccttttagaa attggtggaa ctaagtatga
      181 aaatgaatat ttagacgccg ttgaatactc aatgtccaac atgagcaaag aaatgagatt
      241 aggttattcg tggcatgctt atagagcttg ggaaacaaga tggtccacaa tcattgcttc
      301 aaatcgatta ataataaaaa aacgcatcga agaaattggc gtactggagg atgctaattc
      361 tatagtaaaa gatatagtgt aattgaagag cggcaaaaat ttaacgggca gctacaactg
      421 cccgatatat aaatcaatcc gcattcagga gcaatgcgtt atctatgatg atctgttccc
      481 attcttcgaa tgcccggtcg cggacgccct ggggaacgct gttagttttg aaatcgacga
      541 ccgtccgcca tttcccgtcc ggacggtaca tgcgcagagc tttacttccc ccttccctgc
      601 gcacctcaac gttatgcttg tcagcaaact cttgtaatgc tcgtagcgtc ccatgcttta
      661 ctgtgtagta tcgctttttc aagttttctc tccagcctgt gccaaggctt caacttccaa
      721 atcgtaagac tcaaactcat agtcctggtc gtcaacttct tcaggcactg gcagtaaatg
      781 ccaggctgag tatatctgac cattatcaaa acgctcctgg ctgtagagcg tcgcggctat
      841 gagtgttagc gccgggcggt cataacggta aattttgcga acgtcacggt caacgagacg
      901 accgaaatta ccataaccgc gctccagtaa taatttttta atttccggcc agtatgggcc
      961 atagctgcgg tacaggcggg gatttttcag taatcgccca cgtagccctg acaggaagaa
     1021 atcaacgtat tcgtcttctg tctttcctaa caacgccgta cgcagtaccg cctcaagata
     1081 tgttttattc ggttttattg tatcagatag tgtggccata ttatgcgacg cccggcgaac
     1141 cgggcgctcc tgttatgcgt attgttggat gacggccaga acgtccgcca cgttgtgttt
     1201 tgtctcgata atccaccagt tacccgggaa atcgctgttc ttcgccttcg ctggcagcca
     1261 gcgagcgccg aatttcgcct tgattgcgtc tttcgcacgg aaaagaacgc ctttcatgcc
     1321 tgaggcttcc tgaagcccaa atacctcgcc agcggcgaat tttggtgcgt acatcatctt
     1381 caggtcggcg gtggatacgc gataattcag accaagagac tgagctatgc tggtggcatc
     1441 accctgtatt gatgataact cttcttgttt ctcgtttctg gcggcaattt cttcctccgt
     1501 gatgttgcca agggccaggt ttatccgatc agcgtcggcc tgtttctctt catcggtgcg
     1561 cccggcaaga accgtgttaa ttctctgcaa tatctccaca tgattcttgc gcatgctgag
     1621 caattccggc gtaacctcgt taagatccac cagcccaagg atggcaaggt cggaaaacat
     1681 tgataccagg ttgtaggtca tgcgatagct gagttgacca taggctgatg gcaacttcac
     1741 cgcatccatt tgataggcat ccataaattt agagccgtcg tttacgacat ccgcaattgc
     1801 aggtgtgatt ttccctgtgg tggcggcctc cctgattgct gttacccacg attgagtcag
     1861 tgcggcgact gcatgattca gattggcttc ccgttctgct gcgatacgcg cgcttgctgc
     1921 gtccattgcc tgcttgatct cggctttatt gctgtaaatg ccaatggtgc caaactgtgc
     1981 tgtggtgatc tcataatctg acgcccggaa ctcatgggta ccgaaaatgg cattggtgac
     2041 ctcaagttca gaatccccgt tacgagtagc cccctggctt gttttctccg gcattctggc
     2101 gatcgcatcc gctattttct cctgaattgc ttcaggggat agcgtatctc catatgacgc
     2161 gattacatcg ccataattgg agccaaacag ttcagtcagg aatacttctg ccaaacggac
     2221 ctggcggtta ttcccttcag acatcatacc aagcacccat tttgcaattg acgacttcag
     2281 cgcgccgtca cggcgatccg ggtaaaccgc atgcttcagt gggtccgtat aggtaccaac
     2341 aaaatcaatg ctatagcctg actctgtagt ctgaacgccg tatgagtcag tgattttgat
     2401 catgccgcgc tgctggaaac ggtagaaatc gtcacaggaa atgatgtcgt taatcccggc
     2461 gatggagacg ccaccactga ttttctgcat aacagcatct tcatcgggag ttacatcaac
     2521 ctgtttatcc agcgtcttca catcccagtt acccgatttg gtacctttga aggtaaagat
     2581 aatctccacg tctgcgcgct ggctgtcgaa gtccagcgac ttaatgcgaa cgatatcacc
     2641 ggcacaatcg tagtattggc ctacacgcca tgagcgatcg ccgataataa ggaactcact
     2701 cgcatggtta accagatcag gatcaacatc cagaatgcct ttatttattg catcctccac
     2761 cagcgggcga aggcgtttga tatccgtcgc ggccttctga gtacggttca ataatttctc
     2821 atagcgggag atggcttgag agatattagc cttgcgctga atggcgcttt tcaacgacgc
     2881 gcgatactgt gctaacaacg tacggtctgt gtgatggacg ctaccccagc gggctttcca
     2941 gtctgcgtta tcagctgctt tggccattac cgcctgtttg aatttggcga cgtcggcggt
     3001 ggtcttttcg agttccgctt ttcttcgctc taattcagcg gtaagcacct ccacatcctc
     3061 accagctgcg tgctgcgcct tgatgtagtt ctgaaggtcg atagtagcct gttctttctg
     3121 gcgagcgcgt tgcgcggctt tcgccttatc catttgaacc tgcatcattg ccagacgttc
     3181 gccatcatcc ttagcggtat acatctgcat ttcgatcata tcgttggcgt cggcgttctc
     3241 catttctgac ttatctgaac ggaggatatc ggagatccag cctgctttac gcttcagcgt
     3301 cttcagtcgg tattcatcga aagaaccctt gccgcagtag tagtgaacgc gaacgcttgc
     3361 acggttggaa ccaactcggg caccgcgacc gttacgctgt gcgatactgg ctggtgtcca
     3421 tggcaacgtc agatgatgga tgtcagtcgt tcctcgatgc aggttgatac ccacctctgc
     3481 ctttttgttg cagatgatga tcggagtccg gccctcctgg aagtcggcag caatcttttc
     3541 cagcccgccc agcgacattt cattttgctg cgcgatatag gcgtcataca gagccatttg
     3601 ctcgttgtat ttcgctatct gtgcatctgt tggttcatcc ggtagctctt tcggcggttt
     3661 aaccgctttc agtttcttac cggttttacc tgcctcggca accgtctgag cattcagtat
     3721 ccccaccttt gaaggttcaa ggttaagagc attgcagata atgcgcttga gcttctggtg
     3781 ctgcgttttt tcatcggtga agatgatttg cttaccttcc gggaaaaact ccttcagcgt
     3841 ggcaatcagc ttcgcgtatt tgggcgtaac ggggtgagtt acggtctgtt cgtcaatgcc
     3901 aaacctggcc aggcgcttat tcacttcctg ctcgaacgct tccggaacct gcaactgaat
     3961 aaactcgccc ttatctatca gggagtattg cgattgctgc gtgattgaat catcactgtc
     4021 gtcgtcttcg ctggtggctt gtttaggcaa actgtccgcc agctgctgca ccgcatctgc
     4081 gtactccggc aggaaacgat aggtgatccg gcgatagtac aggtccatgt cagtacatac
     4141 gcggtccata tccctgatta ttgagaagat cggacgggct ttctcgtgct caatcacgcc
     4201 gtcttcattg accgaggtcg ttacgccatt gttggctttg gccgccgctt ccgcctgctg
     4261 acgcaattct tcatacgccg ccagttgttc ttcagtaagt ggtgcatcct gctggtgttc
     4321 gtccagctcc gggatctcca cggtatcctt aacgtcttcc gccgttttaa gcgttaccca
     4381 gcgatggaat ataccgcgca gcgcatcaag gttttcaaag cccaccagcg ccattttttc
     4441 ttcaacttca ccgctgattt tctgtaccgt ttccagcctg gtcttgccga agaatttaac
     4501 gaagtcatca ggaccgtaga tccccatctt ctgccagtat tccttcggca gaacatgaga
     4561 aagcatgttg tatgcatcga tcggggtgtt aacgactggc gttgcagtca ggagaaccgg
     4621 cccgcgcccg ccattctttt tcatcaggta cgcgtttttg attgccatat cccgcgccga
     4681 ttgcgccacc gcgctggtgg gcagataggc cagttgtgac gcttcgcgac catttttata
     4741 gctattgcgg tagttgtgac cttcgtcggc gatcacacta tcgaagccca tatcctcaaa
     4801 gtacggatac ttctctgctt tttcggtacc ggtatctgaa tactccgaca atacccagcg
     4861 acgcgccgcc tctttacgat gggagtcgga atccattgcg ctggctacgc gcccggcggc
     4921 aacgaagtca taaagcatgt cttgtgcatg ctcatctacg gtgtcatcac gtagcggaat
     4981 gcgggcgtat tgttctttgg taaacacgac tgcacggtaa tttgagtgcg ggatcgcgtt
     5041 catccgcgcc gtgatagtgg cttcatctgc cagcttaaga gcatcgcgca taactggagt
     5101 gccatcagta ccaagaacag gtttaccgtt ctcatcgagc accggcacct ggcgaatctg
     5161 atcgccatcc atcagcacat caagaccgac gaacaggtag ttactgaatg cctcttcact
     5221 caggaactct tttgcttcgt aataccagtt ttccagcact gctttaggca ctacataagc
     5281 agtacgggtg gagcgaccgt tctcatagtt gaacgcctca agcgccagcg cggtcgtggt
     5341 tttacccagc ccggtgccga agcccaggat gccgcgccca tcttcggaca gtcggcgcac
     5401 ctcgctattc tggtaatcaa atggctggcg cttaccgctt aatcccttca acccaagcgg
     5461 atcgccagag tgttcatacg ggatattgct attgaacaca tcgttgtatt tggcaaccag
     5521 ctcatcgtag cgatcgtgcg tcttgatcca cttattgaac tggtcctcaa gcagtgccat
     5581 ctgctcgcgg tagccgttcg ccgtcgcgct atctttgcca ccgatacgcg caccattgag
     5641 atacttttcc agctgtgccg ggaacccggt cgcgttttca cctgatttac ggtcccactc
     5701 gtagcggatc tcgcctgttt ctttatcctt gcgctggacg acaccgtatc ggtgcccgac
     5761 gaacagacca tcaccaccgt gataggtgtc agaaaccatt tcgtcgcctt ccagctgcac
     5821 tgactgcaca tagcgcagat ccggatagcc gttttcctgc aaaaactcca gaatgacgga
     5881 gcggtcgaac caacggctat tgagcttaaa gcggatattc tctgctggcg tcttgatgcg
     5941 cttctcttcg atcgctgcca gctgattaag gacgttgttc tttactggac cgtcggggag
     6001 cgtggcgagg aattcctgtt ttggagccac tatctcgtta atgtcgccgc tggtggcgcg
     6061 ggcgaacgga acaatcccgc catacggtga aaccgcaatg ccaggggtgc tggccaataa
     6121 attaagcaac tcatcatcac tggctggcag ttcgccggta aacgcaaggc ggaaatcatc
     6181 gagctggatt ggatcgcgag tgagatcact gtagagataa cgcagggtgt cctgatagct
     6241 ggtggagtca taactggcgc tggaatcatg cgtaaccagt tttcctgtca gctcgtcaga
     6301 aatagtgcca tccagcttaa ttgcaccacg gaaagcaaac caggcgcgcg caccgctccc
     6361 cgataatttc gctatcggac cgcgaccggg gttaccaaaa cggtcaatct ctgcctgcaa
     6421 acgggatacc agagaaaggc gctgctgttc gatttgttca gcactatgcc cggcggcctt
     6481 catgtcctga tattcaatta acatccggcc aatcatcgcc ccgcgataca agcgttcacg
     6541 gtatttttca ggctggctgt taatccagtc caccagctgc accatatcgt cgctgattga
     6601 tgtggtgtac ttatcgcgga catttgccat ctgggtaaat gtcatgccga gacggccttc
     6661 tgttgtagtc aggttacgct gaagagcctc ccagctatcc gcgccataac tggcagcatc
     6721 gatcttaagt tccttcccgg catcagcttc aatccagcga ccaccagcat atttttgcca
     6781 tacgccatta atcaggcgca tttccccttc accaacaacg tctgcggtcg gtgacggttc
     6841 agccatatcg agcaaagacc agtcgatacg gctttcgaaa cgatgaatca gcttcgcttt
     6901 aagagcctgg ttatcaatct gaccgtcggc acgaacctca atacgccccc ggaagccctt
     6961 ttcttgggtg ccatgaacaa accggcggcc atccttttca aaccacttgc cagaaataaa
     7021 cgttggccaa agcacatttg ctgattcaag agtgctttca tccaccaggg ggattttctc
     7081 agccatctct gccggatgtt tgcgcatcag caccacatct acgaccgtac tggtcccgtt
     7141 tgcgtcaaaa gtaccggtag gcaagcggtg ggcaccaaga aattctgctt tccgtgatag
     7201 gcgcaggcgt aaccgcttca tgtttgaacc tgaaacaatg gacggcggca caatcacgca
     7261 catgaatccg cctggcttta tcttgtccag catgcggagc atgaagtaag aacccatgtc
     7321 cgtttcttct gcgtaaggct tatcgatgtt gcgtgtgtta tcacgaccgc cgaacggaac
     7381 gttacccaca acatggtcga atgaatcgtt aggtgtgctt atagccagct gttcgaacgg
     7441 ggaaatctgt acgctgtctt ccgggtgtaa caactggttt atacgaccgg aaacactgct
     7501 gatctcagtc gcagtcatca ccgtaccaac cggttttgtc tcattaaaaa cgccggtgcc
     7561 cgccgatggt tccagagtgt tacctacgtc cgcgccgtag agcttcatga tctcccagac
     7621 accttcagcg atcggctttg gtgtgtaata ttcggagacg gacccgccaa tgccgccttc
     7681 accggtgtac ccagccagga tctggcgctg ttcatctgtc agtgtcgcgc cgtccaccag
     7741 tgaattaagc aaatctatcg ccttctgatt cgcctcccgg cgcagtcggt catagctttt
     7801 gccttccacc ttttccacgc cgtatttaat cggcgctcgg tgagatgtta ttgccctaat
     7861 gtatttcaat atttcgctga cacttgaaca gcgaaacacc cccatagata gcttgttcat
     7921 tggtaatcct taacaagtga ctagtgttaa attccgttca aacacgatgc gaattattct
     7981 aattaaggtg caatcttggc agacaataaa atcacgctat cctcggtcag gaaggcgctg
     8041 gcgggggttt ttaaagacaa cggagaacgg gacaacatcc tcctgtccgc gctggctgtg
     8101 cacggcggaa gtgggtattt gttttctcgc gcaggggcac cggtacaact gtccggcttc
     8161 ttaggcggca aaccgggcga tagtggcatg gctggcgatg ggctggtgga cggaagtcgc
     8221 tttatctttg atgaagttca actgccggaa gaccgcttgc aacgctatcc gctactcgaa
     8281 gagatggcgg tttacagcac gatcgccacc gcgctgaaca tccatattac gcacgcgctc
     8341 tctttcgata agaagaccgg acaaaccttc tctatcgtgc cggtacacaa cggaaacgat
     8401 agtgactatg acgccgcgca ggggttgtgt gacgagctga tgaacgacat cgggcgaacc
     8461 atcaacaaag aggtcgccgg gtgggcattt atcatgtctg tatttggggt ggcttatgtc
     8521 aggccatacg ccaaagaagg catagggatc acgtcttttg agtgctccta ttacaccctt
     8581 ccgagcttca tcaaggagtt cgaggtcagc ggtaacctgg cgggatttag cggcgattat
     8641 ctgaaggacg cgtcagggaa aatggttttc gccgatccgt ggaccattat ccctatgaaa
     8701 atcccctact ggcggcctaa gtcaaacctt atgcctgtgc acactggcca taaggcttac
     8761 agcctgctgg ataatccgga agagcgcacg ccgattgaaa cccagaatta cgggaccagc
     8821 ttgctcgaat acgcctacga gccgtacatg aacctgcgtt cggcgatccg ctcgctgaag
     8881 gcaacgcgtt ttaatgcgtc gaaaattgac cgaatcatcg gtctggcgat gaatagtctg
     8941 gatccggtaa aagcagccga ttattcacgc accattactc agacgcttaa acgagcagct
     9001 gacctgatgg aaaagcgcgc acgcggcgcg aataacatgc ctacggtcac caataccctg
     9061 ctgcctatta tgggcgacgg caaggggcag atgaccattg atactcagac catccaggca
     9121 gacatcaacg gcattgaaga cattctcact tatatgcgcc agctggcggc agcacttggc
     9181 ctcgattaca ccctcctggg gtgggcagat caaatgtccg gcgggcttgg tgaaggtgga
     9241 ttcctgcgca cggcgattca ggccgccatg cgcgcctcat ggatccagca gggcgtagaa
     9301 gagttcattc agcgggctat cgatattcat cttgctttca agtacggcaa ggtatacccg
     9361 gaaggtgatc gcccgtacaa aatcgaattc cactccgtta ataccgctct gcaacaagag
     9421 cacaacgata accgcgactc gcaggcgaac tacgccacca tcgttacgca aatcctcgat
     9481 gccgtcagca ataacagcgt cctcgctaat tccgatgcat tcaaacgtta cctgttcagc
     9541 gatgtgctgg agattgacga aaaaatctct gaagcactgg tgaacgaact gaaagctaaa
     9601 agcgaggacg acgatcacct gatggattcc atcatcaaaa caccgccaca ggaactggcg
     9661 caaatccttg aatcggtctt taaagaggga aacgataatg actgatgttt tgaaaacggt
     9721 cactgaccgc ttttgtctct atagcaatgc tcgaaaaggt cgccagaacg ggcgacagta
     9781 tgtattaagc gcggtaaaga ccatgcttga aagcaaggaa actcaggaag gtttacgcct
     9841 tggtgagctt ttcggctatt acggtcacgg tcgccgacag ctgaccggca aactggaagt
     9901 accagaaacc agcgtgatca tggtggaagg tcgcccggtc gtaatcgaca atgttccagc
     9961 gtgccgaaca gtggctatat ctgttgacga caacggcatc gttacccata cacaggaaat
    10021 tcttaacaca gagccgggta aaattgtcgc cgcgatgatc gaaagccgag ctggtggctg
    10081 gagctgggcc actggcgggc gtgagtccgg gaaaatcgct gtaaccacca gcttccatgg
    10141 tgtggattat gtgacaacgc cgaactatat cagtctggat catcctgcca gcgccggaat
    10201 gtttgaaagc gcggattcta aatctctact ggcagagtcc ctggcggcgc atggatactc
    10261 tgacgagtca gtgcaggccg ttatatccca ttacagtaaa atggctgaac tggaaatgat
    10321 ggtggaggcg acagagcgta cggctgaact ggaaaccgca ctactcgaaa gccagggtcg
    10381 ccaccttgaa gcaatggcca agatcgcaga tgctgaagcg cgaatcgctt tgctggagaa
    10441 aacagcgggt atccgcgacg atgtgctggc agcgatgcaa gacgaactgg ataacctccc
    10501 gattttcgtc tccgccgccc aaaaagacgc attccgcctc aaagaacctg gtgatgcaaa
    10561 aatcgtggcc acgcttttcg aatctctgat caaagttggc gcacgcaact tgcctgtcac
    10621 caagaaaatt aaggaggttc cgcaagcggc taacgtccag gcaccgcgtg agacaagcat
    10681 catcacgttt aataattcaa tcaacccgtt taaataacca tcaaaaataa ccccggcggc
    10741 tgccggggtt atcgttaact attatcgcct tcgtctgcgt gccatatatt tgcgcaccgc
    10801 gcgacgtggg caatctgaag cggtttcttt ctgctgcatc aatcttgcag ccatgctcaa
    10861 aaatgtcagg cacagccgaa gcccggcata taatagcggt tccagtggcc acgtctcatt
    10921 gagcacatat accgccatga aaatcgagtc gaaaactatc gccgccagcg ataacttcat
    10981 tgtcgaaagt cggcggagct gccggagttt attcattgat cagccccgtc aggcaaagct
    11041 ggcgttcttt ttcacggcga atctttaaac ctcgcagggg cacgccgtta ctgttcacaa
    11101 aatcagggag atggttacac atattcaccc attccccttt ctgcgcccac ttgtggatgg
    11161 acgtttcgac tcgcatgcct cgcgctttgc tgtagtaggt ccgtaagcta ttgcatccca
    11221 tattgaatgc cgcgcttgtc attgcactga aggcattatc gggcatgtct ttgccccgaa
    11281 agtgctgatt aatacagcgt tcagcgatca ggatattctt ttcccaatca gcggcgattt
    11341 gctggtcggt ttttcgcaca cccggcgtta ccccgtgtgt attaccgatc ccgtcagtcc
    11401 atacccccgc cgggcacatg tatggatcac gtcggcaacc ttcagcgtta ccaatcagct
    11461 caagccccgc ctggttggtt cgcacattgc cattacccat cacgatggta atcatcaccg
    11521 cgatagcgca aattgcaccg cctcctgcgg ctgtttttcc cttcataaag acctcataag
    11581 cgaatttttt acgctccagg acaaacaccc attcacagcc aataccgact gactcgatcc
    11641 ctttagaagg cacaggataa tgcaaatcac ttgttagcta cgtttcaaag atatacattg
    11701 ttgctctaat tattttattt tattaggtaa gataagtggc acaacgcggt gtaaacaaag
    11761 tcatcctgat tggcaccctg gggcaagacc cggagatcag gtatatacca aatggcggcg
    11821 cggtcggaag actcagcatc gcaacgaatg aatcatggcg cgacaagcaa acgggccaac
    11881 agaaagagca aacagaatgg cataaagtcg ttttgttcgg aaaacttgct gaaattgcga
    11941 gtgagtattt acgaaaaggt tctcaggtct acatcgaagg gaaacttaaa acccgtaagt
    12001 ggacagatga cgccggtgta gaacgttaca cgacggaaat tatcgtcagc cagggcggca
    12061 ccatgcaaat gatcggcgct cgtcgcgacg attcacagtc cacaaatggc tgggggcaat
    12121 caaaccaacc tcaaaaccac cagcaataca gtggtggcgg taaacctcag agcaacgcta
    12181 ataacgaacc tccaatggac tttgacgacg atattccgtt ttgaatgtgt aaaaaacgac
    12241 tgaaaaaaaa gcggtggtcc agacgccgac aaaagcacga actcgctcaa aaacgccaaa
    12301 gttggcaatg gcacgcgctt ttaacgaata gaacaccccg agatattgct ttcgctggtg
    12361 ggaaaacatt cctaaccaac ctgaaggcgc aatacatcag tttttaagca gagaaaagac
    12421 tatgacagca caaaatacta aaaccattca ataccgccta cgtaatggcc agagtgtcga
    12481 agtgaccgtc aataatgatg gagtgccagg cgaaacggtt tctatctctg atctggctat
    12541 cgaaaaaacc atcatgtgcc accttggctt tactgaagaa gtgagcaaaa agcatggtgt
    12601 agcaatctgg agcgcaatgg atactggtat gcgcagattc attactgctc gtaccccagg
    12661 gatgaccatg atggacctca tgcagattgc gccgctgttt gagtgtgaac ctttggatgt
    12721 attcagcaat ccagctatct gccagcagtt atatggtgag atgaaactcg cggttacccc
    12781 cattgtgctg catgaaggat cgcttgctgg cgtatggaaa gtagagcgaa tttcaagcta
    12841 catgcctttc catgtcaacg gcgtaatcac tggtgaaaat caacctgttt ccgttataaa
    12901 gtcagacctc aagcgcgcaa ttcttgaagc aagttgtcga gttgtcggcc tgggcaaaca
    12961 gtcttatgtt tccttcccgg ctggccctga aggcccggca gaaattctga ttatggatgc
    13021 cgatctgctc tggcaaatac agtttctgat tggcaaaagc atcatccgcg atgaagaact
    13081 agatcagtac attacctgca cgatgacgga tgaagtcaaa agtgtggcta tagccaatgc
    13141 ccggaaccta tgtcgtgctg cattaacaga actgcaagaa aacaccacgg gagaagtgga
    13201 aagtgattaa aaagaacccg ccaattggcg ggttctttat ttattgaatt ag
//
LOCUS       DAZUFB010000127        13060 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZUFB010000127 DAZUFB010000000
VERSION     DAZUFB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.127"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            137..1246
                     /gene="livK"
                     /locus_tag="ACHY3M_004216"
     CDS             137..1246
                     /gene="livK"
                     /locus_tag="ACHY3M_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFS4319025.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1294..2220
                     /gene="livH"
                     /locus_tag="ACHY3M_004217"
     CDS             1294..2220
                     /gene="livH"
                     /locus_tag="ACHY3M_004217"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFS4319026.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2217..3494
                     /gene="livM"
                     /locus_tag="ACHY3M_004218"
     CDS             2217..3494
                     /gene="livM"
                     /locus_tag="ACHY3M_004218"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFS4319027.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVV
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3491..4258
                     /gene="livG"
                     /locus_tag="ACHY3M_004219"
     CDS             3491..4258
                     /gene="livG"
                     /locus_tag="ACHY3M_004219"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFS4319028.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4260..4973
                     /gene="livF"
                     /locus_tag="ACHY3M_004220"
     CDS             4260..4973
                     /gene="livF"
                     /locus_tag="ACHY3M_004220"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFS4319029.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5369..6685
                     /gene="ugpB"
                     /locus_tag="ACHY3M_004221"
     CDS             5369..6685
                     /gene="ugpB"
                     /locus_tag="ACHY3M_004221"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFS4319030.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6783..7670
                     /gene="ugpA"
                     /locus_tag="ACHY3M_004222"
     CDS             6783..7670
                     /gene="ugpA"
                     /locus_tag="ACHY3M_004222"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFS4319031.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7667..8512
                     /gene="ugpE"
                     /locus_tag="ACHY3M_004223"
     CDS             7667..8512
                     /gene="ugpE"
                     /locus_tag="ACHY3M_004223"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFS4319032.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRVL
                     VDSEK"
     gene            8514..9584
                     /gene="ugpC"
                     /locus_tag="ACHY3M_004224"
     CDS             8514..9584
                     /gene="ugpC"
                     /locus_tag="ACHY3M_004224"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFS4319033.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9581..10324
                     /gene="ugpQ"
                     /locus_tag="ACHY3M_004225"
     CDS             9581..10324
                     /gene="ugpQ"
                     /locus_tag="ACHY3M_004225"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4319034.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10311..10751)
                     /locus_tag="ACHY3M_004226"
     CDS             complement(10311..10751)
                     /locus_tag="ACHY3M_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFS4319035.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10871..12613
                     /gene="ggt"
                     /locus_tag="ACHY3M_004227"
     CDS             10871..12613
                     /gene="ggt"
                     /locus_tag="ACHY3M_004227"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFS4319036.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12651..12935)
                     /locus_tag="ACHY3M_004228"
     CDS             complement(12651..12935)
                     /locus_tag="ACHY3M_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFS4319037.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2897 a         3332 c         3745 g         3086 t
ORIGIN      
        1 atcactatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa aacagtgcag
       61 tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac aacatcacga
      121 atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact
      181 ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg gcgcgatgtc
      241 cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga
      301 cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat atgacgacgc
      361 ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg gcattaaata
      421 cgttattggt catctgtgtt cttcttctac ccagcctgcg tcagatatct atgaagacga
      481 aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac gcggttatca
      541 acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg caaaatacat
      601 tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac aatatggcga
      661 agggctggca cgttcggtgc aggacgggct gaaagctgct aacgccaatg ttgttttctt
      721 cgacggcatt accgctggcg agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga
      781 aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca
      841 ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtgg gtaatgcgtc
      901 gctgtcgaat attgctggcg atgctgccga aggcatgttg gtcaccatgc caaaacgcta
      961 tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca agaaagatcc
     1021 gtccgggcct tatgtctgga tcacttacgc ggcggtgcaa tctctggcaa cagcccttga
     1081 gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa
     1141 caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg
     1201 tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat cccaccgccc
     1261 gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt gtatttcttg
     1321 cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc catcggctac
     1381 accatggttt acggcattat cggcatgata aacttcgccc acggcgaggt ttatatgatt
     1441 ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat tgataccggc
     1501 tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg
     1561 agtatcgaac gggtggccta tcgcccggtg cgtaactcta agcgcctgat tgcgctcatc
     1621 tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga aggttcgcgt
     1681 gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tgggccatag cgaaaacttc
     1741 tctgcctcta ttactaccat gcaggcggtg atctggattg tcaccttcct cgccatgctg
     1801 gcgctgacga ttttcattcg ctattcccgt atggggcgcg cgtgtcgtgc ctgcgcggaa
     1861 gatctgaaaa tggcgagtct gcttggcatt aacaccgacc gggtgattgc gctgaccttt
     1921 gtgattggcg cggcgatggc ggcggtggcg ggtgtgctgc tcggtcagtt ctacggcgta
     1981 attaacccct acatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt
     2041 gggattggca gcattccagg ggcgatgatt ggtgggctga tcctggggat tgcggaggcg
     2101 ctctcttctg cctatctgag tacggaatat aaggatgtgg tttcatttgc cctgctgatt
     2161 ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga
     2221 aaccgatgca tattgcaatg gcgctgctct ctgccgcgat gttttttgtc ctggcgggcg
     2281 tctttatggg cgtgcaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag
     2341 acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc cagcttttgc
     2401 gaccggcttt ccagaaaggg ctaaaaagcg tttccggacc gaagtttatt ctgcccgcga
     2461 ttgatggctc cacggtgaag cagaaactat tcctcgtggc gctgctggtg cttgcggtgg
     2521 tgtggccgtt tatggtgtcg cgtgggacgg tggatatcgc caccctgacc atgatctaca
     2581 ttatcctcgg cctcgggctg aacgtggttg ttggtctttc tggtctgctg gtgctggggt
     2641 acggcggctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac tattacggcc
     2701 tgggcttctg gacctgtctg ccgattgccg ggttaatggc agcggcggcc ggcttcctgc
     2761 tcggcttccc ggtgcttcgc ttgcgcggcg actatctggc gatcgttacc ctcggtttcg
     2821 gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc ccgaacggaa
     2881 tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc gctcgtgaag
     2941 gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgtgtca
     3001 tcttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc attaaccgcc
     3061 tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagacgag atcgcctgcc
     3121 gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccata agtgccgcgt
     3181 ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc ccggaatcct
     3241 tcacctttgc tgaatcggcg tttgtactgg cgatagtggt gctcggcggt atgggatcgc
     3301 agtttgcggt gattctggcg gcaattttgc tggtggtatc gcgcgaattg atgcgtgatt
     3361 tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc
     3421 cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag
     3481 gagagcaggc atgagtcagc cattattatc tgttaacggt ctgatgatgc gcttcggcgg
     3541 cttgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg tctcgttaat
     3601 cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggat tctacaaacc
     3661 caccggcggc actattttac tgcgtgatca gcatctggaa ggtttaccgg ggcagcaaat
     3721 tgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgtg aaatgacggt
     3781 gattgaaaac ctgctggtgg cgcagcatca acaactgaaa accgggctgt tctctggcct
     3841 gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg ccgccacctg
     3901 gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc tggcctatgg
     3961 tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga ttttaatgct
     4021 cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg agctgattgc
     4081 cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata tgaagctggt
     4141 gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg caaacggtac
     4201 gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga
     4261 tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa atccaggcgc
     4321 tgcatgaggt gagcctgcat atcaatcagg gcgagattgt caccctcatt ggcgcgaacg
     4381 gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgct accagcgggc
     4441 gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag
     4501 cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc
     4561 tggcgatggg cggctttttt gccgaacgcg accagttcca ggagcgcata aagtgggtgt
     4621 atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcaggcacc atgtccggcg
     4681 gtgagcaaca aatgctggcg attggtcgcg cgctgatgag caacccgcgt ttgctgctac
     4741 ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc gacaccatcg
     4801 agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc aaccaggcgc
     4861 taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc tccgataccg
     4921 gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg taataacacg
     4981 ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa aattgcctga
     5041 tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg cacgatcttg
     5101 taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgagtaat ctgtagagtt
     5161 tgattcagac cttctatttt ccgcttatcc gtgccccatc tcccattttc cctcacccac
     5221 gccgtcaccg ccttgtcatc tttctgacac cttaccatct tacaaatgta acaaaaaagt
     5281 tatttttctg taattcgagc atgtcatgtt accccgcgag cataaaacgc gtgaattcgc
     5341 gcattcggta caacaagaga gataaacgat gaaaccgtta cattatacag cttcagcact
     5401 ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg acgaccattc cgttctggca
     5461 ttctatggaa ggggaactgg gtaaagaggt ggattctctg gcccaacgtt ttaacgccga
     5521 aaatccggat tacaaaattg taccgaccta taaaggcaac tacgaacaga atttaagcgc
     5581 ggggattgcc gcatttcgta ccggcaacgc tccggctatt ttgcaggttt atgaagttgg
     5641 caccgccacc atgatggcat cgaaagccat taaaccggta tatgacgtgt ttaaagaggc
     5701 ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt tccggttact actccgacag
     5761 caaaacgggc cacttactct cccagccatt caacagctcg acccccgttc tctattacaa
     5821 caaagacgcc ttcaagaaag caggattaga cccggaacaa ccgccgaaaa cctggcagga
     5881 tctggcggac tatgccgcga aactgaaagc ctccggtatg aagtgcggct acgccagcgg
     5941 ctggcagggc tggatccaac tggaaaactt tagcgcctgg aacggtctgc cgtttgccag
     6001 caaaaacaac ggctttgacg gcacggacgc ggtgctggag ttcaacaagc cggagcaggt
     6061 gaaacatatc gccatgctcg aggagatgaa caagaagggc gacttcagct acgtcggtcg
     6121 taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacca ccgcctcttc
     6181 cggttctctt gccaacattc gcgagtacgc caaatttaat tacggcgtag gcatgatgcc
     6241 ttacgacgcc gatgcgaaag atgcgccaca aaacgccatt atcggcggag ccagcctgtg
     6301 ggtgatgcag ggtaaagata aagaaacgta taccggtgtg gcgaagttcc tcgatttcct
     6361 cgcgaagcca gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc caattaccaa
     6421 agcggcgtat gacctgaccc gtgagcaggg cttttacgag aaaaacccag gtgcggatac
     6481 cgcgacgcgt cagatgctga ataagccgcc gttgccgttc accaaagggc tgcgtctggg
     6541 caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt ggaccggtaa
     6601 gaagacacca cagcaggcac tggataccgc cgttgagcgt ggaaatcagt tgctgcgccg
     6661 ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg cttatctgac
     6721 ctggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag gaattaaccg
     6781 taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatctg ctggtcgcgc
     6841 cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg ttgtggtact
     6901 cgctacaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg gataacttcg
     6961 tcgcgctgtt tcatgacagc tactatctcg actccttctg gacgacgata aaattcagca
     7021 cctttgtcac cgtcagcggt ttgctggtgt cgctgttctt cgcggcgctg gtggagtaca
     7081 tcgtgcgcgg cagccgtttc tatcaaacct taatgctgct gccttacgcc gtggctcccg
     7141 ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg atcacccatt
     7201 ttctcgcgga gttcggctac gactggaacc acgcgcaaaa cagcggtcag gcaatgtttc
     7261 tggtggtgtt tgcctcagta tggaagcaaa tcagctacaa cttcctgttc ttctatgccg
     7321 cgctgcaatc cattccccgt tcgttgatcg aagccgcagc catcgacggt gcaggcccaa
     7381 ttcgccgctt ctttaagatt gcgctgccgc ttatcgctcc ggtgagtttc ttcctgctgg
     7441 tggtgaacct ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc gccacgtccg
     7501 gcgggccggt tcaggccacc acgacgctga tttataagat ctaccgtgaa ggctttaccg
     7561 gactggatct ggcttcgtct gccgcgcagt cggtggtgtt gatgttcctc gtcatcgtgc
     7621 tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga ttgagaaccg
     7681 tccgtggctg acgatattca gccataccat gctgatcctc gggatcgcgg tgatcctctt
     7741 cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caggccgtct atgccgcgcc
     7801 gatgacgctc atccccggca cacatctgct ggaaaatatc cacaacatct gggttaacgg
     7861 ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg tgatggcgtt
     7921 cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca ttgtctggtt
     7981 tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga tgctgccggt
     8041 tgaagtacgt atcttcccga cggtggaagt catcgccaac ctgaagatgc tcgacagcta
     8101 cgccggttta acgctgccgc tgatggcctc ggcgaccgcc acgtttctgt ttcgccagtt
     8161 ctttatgacg ctgccggatg aactggtgga agcggcgcgg atcgacggcg cgtcaccaat
     8221 gcgtttcttt tgcgacatcg tttttccgct ctccaaaacc aatctggcgg cgctgtttgt
     8281 gatcaccttt atttacggct ggaaccagta tttgtggccg ttgttgatta ttaccgatgt
     8341 ggatctcggc accaccgtgg cagggatcaa agggatgatc gctacaggcg aaggcaccac
     8401 tgaatggaac tcagtgatgg cggcgatgtt gttaacgctt atccctccgg tggtgattgt
     8461 tttagtgatg cagcgtgcct tcgtgcgcgt cctggtcgat agtgagaaat aagatggcag
     8521 gactgaaatt acaggcagta accaaaagct gggatggtaa aacccaggtc attaaaccgc
     8581 tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct ggctgcggga
     8641 aatcgacgct gctgcgcatg gttgccgggc tggagcgggt gacagaaggc gatatctgga
     8701 tcaacgacca gcgcgtgact gaaatggagc caaaagatcg cgggattgcg atggtgttcc
     8761 agaactacgc gctttatccg catatgagtg tcgaagaaaa catggcgtgg gggctgaaaa
     8821 ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg cgcattctgg
     8881 agctggacgg tctgctcaaa cgtcgcccgc gcgagctttc cggcggtcag cgccagcgtg
     8941 tggcgatggg ccgcgcgatt gtgcgcgatc cggcggtgtt cctgtttgat gagccgctct
     9001 ctaacctcga tgccaagctg cgcgtgcaga tgcgtcttga actgcaacag ttgcaccgtc
     9061 gcctgaaaac gacttcactc tacgttactc acgatcaggt tgaagcgatg acgctcgccc
     9121 agcgagtaat ggtgatgaac ggcggcgttg ccgaacagat tggcacacca gttgaagtct
     9181 acgaaaagcc cgccagcctg tttgtagcga gttttatcgg cagtccggcg atgaacctgc
     9241 tgacaggccg cgtgaataac gaaggcacgc atttcgaact ggacggcggt attgagctgc
     9301 cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gactctcggc attcgcccgg
     9361 aacatattgc gctaagctcg caggcagaag gcggcgtacc gctggtgatg gacacgctgg
     9421 agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgagcagaag ctggtggtgc
     9481 gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat ctggcggaaa
     9541 atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact ggccttatcc
     9601 ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg gaaaacaccc tggcggcaat
     9661 cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt tatcgaaaga
     9721 tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc accagcaacg gctggggcgt
     9781 cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt ggtacagcaa
     9841 agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct gtcgcgaaca
     9901 cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat taacgggcaa
     9961 aatggtggcg ctggcggcac gccaactgtg ggccggtatg acgccgccgc tgctgtcatc
    10021 gtttgagatt gatgctttag aagctgcaca gctggcggca ccggaactgc cgcgtggttt
    10081 attgcttgat gagtggcgcg acgactggcg cgaactgacc gcgcggctgg gctgcgtctc
    10141 tattcatctc aatcataagt tactcgataa agtgcgagtg atgcagttga aagacgccgg
    10201 actgtggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt tgctgcgctg
    10261 gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact ttacggccca
    10321 atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat gtcgccgttt
    10381 ctcctctccg gcagcatatg ctgctgattc aacgaactat ccggattacg gttgctgttt
    10441 aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg ctcccccggc
    10501 tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg ctgctgttgc
    10561 aactgagttt gcgttttcag ttgctgattc agcatccctt tttgctggat ttgctgagtc
    10621 tgcatctggg tttgcatccg ctgctggctg ggtatctgat accccggctg gttagggttg
    10681 ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt aagaatcaga
    10741 agtcgtttca tcgcgtatcc tcctctgaag atatccttta agtttactcg cttcccgaca
    10801 aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc ttaatctctg
    10861 gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg ctctgctctc
    10921 aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg cccgtctcgt atggtgtgga
    10981 ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg gtagcgtctg tggacgccac
    11041 tgccactcag gtgggggtgg atattctcaa ggagggcggg aatgccgttg atgccgccgt
    11101 ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca gggaatctgg gcggtggtgg
    11161 ttttatgctg atccgctcga aaaatggcaa taccaccgct atcgatttcc gcgaaatggc
    11221 acccgctaaa gcgacccgcg atatgttcct tgatgaccag ggcaacccgg acagcaaaaa
    11281 atcactcact tcgcatctgg cttccggcac accaggtacg gtcgctggtt tctcgctggc
    11341 gttggataag tacggcacta tgccgttgaa caaagtggtg cagcccgcgt tcaaactggc
    11401 acgcgatggt tttatcgtta acgatgcttt ggcagacgat ctcaaaacct acggtagcga
    11461 agtgttgccg aatcacgaaa acagcaaagc tatcttctgg aaagagggcg aaccgctgaa
    11521 aaagggcgac acgctggtgc aggcgaacct ggcaaagagc ctggagatga ttgctgaaaa
    11581 cggcccggac gaattctata aaggcacgat tgcggaacag atcgcccagg agatgcagaa
    11641 aaacggtggc ttgatcacta aagaagattt agcggcctat aaagcggtcg aacgcactcc
    11701 gataagcggc gattatcgcg ggtatcaggt ttactccatg ccaccgccat cctccggcgg
    11761 gatccatatc gtacaaatcc tcaatattct ggaaaacttc gatatgaaga aatacggctt
    11821 tggcagcgcc gatgcgatgc aaatcatggc agaagcggag aaatacgcct acgccgaccg
    11881 ctcggaatat cttggcgacc cggattttgt caaagtcccg tggcaggcac tgaccaataa
    11941 agcctatgcc aaatccattg ccgatcaaat tgatatcaac aaagcgaagc cgtccagtga
    12001 gattcgccct ggcaagcttg cgccttatga gagtaatcaa actacccatt actctgtagt
    12061 ggataaagac ggtaacgcgg tggcggtgac ctatacgctg aacaccacct tcggtacggg
    12121 tattgtcgcg ggcgagagcg gtattctgct taataaccag atggatgatt tctccgccaa
    12181 accgggcgta ccgaacgttt acgggctggt gggcggtgat gccaacgccg tcgggccgaa
    12241 caaacgcccg ctgtcgtcga tgtcgccgac cattgtggtg aaagacggta aaacctggct
    12301 ggttaccggt agcccaggcg gtagccggat catcactaca gtgctgcaaa tggtggtgaa
    12361 tagcatcgat tatggcatga acgtcgccga agcgaccaat gcgccgcgtt tccaccatca
    12421 gtggttgccg gacgagctgc gtgtcgaaaa agggtttagc ccggatacgc tcaagctgct
    12481 ggaagcaaaa ggtcagaaag tggcgctgaa agaggcgatg ggcagtacac aaagcattat
    12541 ggttgggccg gacggtgagt tgtacggcgc atccgacccg cgctcggtgg atgatttaac
    12601 ggcggggtac taaggttagc ggccctcttc gtgggaagag ggctattttg tcagggcaag
    12661 ccgaaggtag ccttttttat ttcgtaatcc tgtagatatt cttccagcgg ctttgccgtt
    12721 cccagagaat gaatttcacc actgtcttta tcaataataa aaggtgcgtt accagctaag
    12781 cgcgcggcct catctccagt ttcgagaaat tctcgtgctt cgaaacagaa ataccagccc
    12841 tggctaaagc gtccatgtag agtaatgacg accgggagat ctgcatcatc aaggtaatgg
    12901 ttcgctttcg cgaatgcgtc gtgataagta atcataatta taataaatat tattgttgag
    12961 tgttatatta ttattcctgt gtgatacatt gagcaaagac gcgttcatct tcgtcaaaga
    13021 tattattaaa tgctggtttt gaaaaaagtt catcggataa
//
LOCUS       DAZUFB010000128        12507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZUFB010000128 DAZUFB010000000
VERSION     DAZUFB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.128"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..321)
                     /gene="yehE"
                     /locus_tag="ACHY3M_004229"
     CDS             complement(40..321)
                     /gene="yehE"
                     /locus_tag="ACHY3M_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFS4319038.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(584..1693)
                     /gene="apbC"
                     /locus_tag="ACHY3M_004230"
     CDS             complement(584..1693)
                     /gene="apbC"
                     /locus_tag="ACHY3M_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFS4319039.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1825..3858
                     /gene="metG"
                     /locus_tag="ACHY3M_004231"
     CDS             1825..3858
                     /gene="metG"
                     /locus_tag="ACHY3M_004231"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4319040.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3999..7793
                     /locus_tag="ACHY3M_004232"
                     /pseudo
     CDS             3999..7793
                     /locus_tag="ACHY3M_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7803..11435
                     /gene="yehI"
                     /locus_tag="ACHY3M_004233"
     CDS             7803..11435
                     /gene="yehI"
                     /locus_tag="ACHY3M_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFS4319041.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQEGLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIGLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11497..11814
                     /gene="yehK"
                     /locus_tag="ACHY3M_004234"
     CDS             11497..11814
                     /gene="yehK"
                     /locus_tag="ACHY3M_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFS4319042.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3270 a         3172 c         3067 g         2998 t
ORIGIN      
        1 tttcggcttt ctaaatgcaa ttaaaatcag gttgccaaat taatcgtagc gatttaacac
       61 gattcgacgt ttgctatcgc tacctgcaac aaccacttct gtcgttacac ctttgacggg
      121 cgaagaaaac agatgacggg tgtcaacatt atacgttaac tgaccacaat gctgttgagg
      181 ttgaccatta accatagcca tactgcatgt tgggggactg atcctgccat taatcgctaa
      241 tgtggcagtc tctgaagaga atgcgggaga agccaatcca taggcaagaa aaataattcc
      301 tgacagccaa tacttattca ttgaacgtta tccctgtagt aaaggttatg cctggataga
      361 atgagtgcat aacaaactat agctgtacat ccactacaca gccacgaagg atgataatga
      421 agcattgcct gtatgatcaa tcgactttgt agagtttcgg acgaaggtcc gcagaatatt
      481 cgcagtatta aataagtctt cattaatgcg gcaaaaacta atacaccgca tcaatgtaac
      541 atctctgcgg tgtatgaaga ataaccagaa tggttaatag gcgttagacc gcgcggaagg
      601 aaatctcgcc tggaatgact tcaccctgcc agtagagctg agctgcaacg cggtcagcca
      661 gctggcggta gatagcggta aattcgctct ccggacggct gataaccgtt ggcgtgcctt
      721 tatcgagatc ctcacgtaag gagatgtgta acggcatctg ccccagcagc tgtgtgtggt
      781 atttttcagc cagtttctca gcaccaccgg taccgaaaat cggttcatgg tgaccacagt
      841 tactgcaaat atgcacactc atgttctcga caataccaag caccggaacc tcgactttct
      901 cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat gtcctgcggg gtggtgacca
      961 cgactgcacc agttactgga atgttttgcg ccagcgtcaa ttgaatgtca ccggtgcctg
     1021 gcggcatatc aagcaccaga tagtcgagat ccggccacaa ggtttcctgc aacatctgca
     1081 ttaacgcctt gctcgccatc ggtccacgcc acaccatcgc attgtcgtca gtgaccagat
     1141 aaccaattga attggttgcc aggccatgag acataatcgg tgccatgtgg gtaccgtccg
     1201 gcgacgttgg acgctgattt tctgcgccca gcatggtggg aattgatggg ccgtagatat
     1261 cggcgtccag aatgccgact ttcgcccctt ccgccgccag tgccagcgcc aggtttaccg
     1321 ccgtggagga tttacccacg ccgcctttac cggaactgac cgcaatgatg ttcttcacac
     1381 cattaatacc cggctgattc ttcacgcgtt ttaaggtggc gatattgtgc gacagtttcc
     1441 agtcgatagc tttcgccccg gtgatacgca gcagttcggc gctacattgc tcttttaact
     1501 cttcaaacgc gctatgccag acgaatggca taaccagttc cacatgcaac gtgtcgtcca
     1561 tccaggcgac atggtgcaac gctttcagcg tggtgaggtt atgcttcagg gttgggtgct
     1621 gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc ttctggtgat ttggcctggg
     1681 attgttcgtt catcccgact ccttttttgt atagataaac catcagctga tagtttacct
     1741 gaagaatata gagaagtact tacttaacat tttcccattt ggtactatct aacccctttt
     1801 cactattaag aagtaatgcc tactatgact caagtcgcga agaaaattct ggtgacgtgc
     1861 gcactgccgt acgctaacgg ctcaatccac ctcggccata tgctggagca catccaggct
     1921 gatgtctggg tccgttacca gcgaatgcgc ggccacgagg tcaacttcat ctgcgccgac
     1981 gatgcccacg gtacaccgat catgctgaaa gctcagcagc tcggtatcac cccggagcag
     2041 atgattggcg aaatgagtca ggagcatcag actgatttcg caggctttaa catcagctat
     2101 gacaactatc actcgacgca cagcgaagag aaccgccagt tgtcagaact tatctactct
     2161 cgcctgaaag aaaacggttt tattaaaaac cgcaccatct ctcagctgta cgatccggaa
     2221 aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct gcccgaaatg taaatccccg
     2281 gatcaatacg gcgataactg cgaagtctgc ggcgcgacct acagcccgac tgaattgatc
     2341 gagccgaaat cggtggtttc tggcgctacg ccggtaatgc gtgattctga acacttcttc
     2401 tttgatctgc cctctttcag cgaaatgttg caggcatgga cccgcagcgg tgcgttgcag
     2461 gagcaggtgg caaataaaat gcaggagtgg tttgaatccg gcctgcaaca gtgggatatc
     2521 tcccgcgacg cgccatactt tggttttgaa attccgaacg cgccgggcaa atatttctac
     2581 gtctggctgg acgcaccgat tggctacatg ggttctttca agaatctgtg cgacaagcgc
     2641 ggcgacagcg taagcttcga tgaatactgg aagaaagact ccaccgccga gctgtaccac
     2701 ttcatcggta aagatattgt ttacttccac agcctgttct ggcctgccat gctggaaggc
     2761 agcaacttcc gcaagccgac caacctgttt gttcatggct atgtgacggt gaacggcgca
     2821 aagatgtcca agtctcgcgg cacctttatt aaagccagca cctggctgaa tcattttgac
     2881 gctgacagcc tgcgttacta ctacactgcg aaactctctt cgcgcattga tgatatcgat
     2941 ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata tcgtgaacaa agtggttaac
     3001 ctggcctctc gtaatgcggg ctttatcaac aagcgttttg acggcgtgct ggcaagcgaa
     3061 ctggctgacc cgcagttgta caaaaccttc actgatgccg ctgaagtgat tggtgaagcg
     3121 tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca tggcgctggc tgatctggct
     3181 aaccgctatg tcgacgagca ggctccgtgg gtggtggcaa aacaggaagg ccgcgatgcc
     3241 gacctgcagg caatttgctc aatgggcata aacctgttcc gcgtgctgat gacttacctg
     3301 aagccggtac tgccgaaact gaccgagcgc gcagaagcat tcctcaatac agaactgacc
     3361 tgggatggta tccagcaacc gctgctgggc cacaaagtga atccgttcaa agcgctgtat
     3421 aaccgcatcg atatgaagca ggttgaagca ctggtggaag cgtctaaaga agaagtgaaa
     3481 gcagctgccg cgccagttac tggcccactg gcagatgatc cgattcagga aaccatcacc
     3541 tttgacgact tcgcaaaagt tgacctgcgc gtggcgctga tcgaaaacgc agagtttgtt
     3601 gaaggttctg acaaactgct gcgcctgaca ctggatctcg gcggtgaaaa acgcaatgtc
     3661 ttctccggta ttcgttctgc ctacccggat ccgcaggcac tgatcgggcg tcacaccatt
     3721 atggtggcca acctggcacc gcgtaaaatg cgctttggta tctccgaagg catggtaatg
     3781 gctgctggtc ctggcgggaa agatattttc ctgctaagcc cggatgccgg tgctaaaccg
     3841 ggtcatcagg tgaaataatc ccccttcaag gcgctgcatc gacagcgcct tttctttata
     3901 aattcctaaa gttgttttct tgcgattttg tctctctcta acccgcataa atactggtag
     3961 catcagcatt caactggata aaattacagg gatgcagaat gaaacacttt atctatcagg
     4021 acgaaaaatc acataaattc tgggcagttg agcaacaggg aaacgagttg catatcagtt
     4081 ggggaaaagt tggcaccaaa gggcaaagcc agataaaaag tttttcagat gctgcggcag
     4141 cggcaaaagc ggagcttaag ctgattgcgg agaaggtgaa gaaggggtat gtggagcaag
     4201 cgaaggataa ttctttgcaa ccttcccaaa cggtaacggg ctctctcaag gtagcggact
     4261 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg cctgacaaaa
     4321 atacagatgc tgttttaccg tggctggcga aagatattgc tgtcgttttt ccgcccgaag
     4381 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
     4441 aattgaccca attacgtcgc ttagcctgta gtgtgtcgca acgggataat aaaacagcca
     4501 catttgactt cagcgcctgt tctttagaat ggcaaaacac cgtcgcccag gcgatcagtc
     4561 agatcgacgg cctgaaaaca acacagctac catcaccagt aatggctgta ctcacggcac
     4621 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
     4681 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattaaat
     4741 gggattatgc gaataatgtc gttattattc tgccgtctgg cattgcacct gactacttgg
     4801 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
     4861 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
     4921 aaccattaat tgctttgtta ttacctgaaa aaccagaaat tgcacatgaa attgcccagc
     4981 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
     5041 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
     5101 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
     5161 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     5221 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgga
     5281 tgacgaaagc cattgctgcg ttcccacatg cagcaatggc ggcactgacg gaacttcttg
     5341 ggcaaaaaga agagaacagt tggcgcatta tgctaatgac aatgcttatc taacaaccag
     5401 cactggcaga acaggtcatt ccctggctct cgacacccgc agttgccgta ctgaaatcat
     5461 gccagcaaca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccgtag
     5521 tagtctcccc tccctggctt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
     5581 cgccattagg cattgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
     5641 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
     5701 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     5761 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     5821 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggaga
     5881 aagcagtaaa agcatggaat gcccttaccc tatctccaca taccgatacc gaatacgcca
     5941 tgttacattt tggactcaaa gggttacctg ggttagtaaa ctcacttgca cgctatccac
     6001 aagaagcctt gcccatcacg aattacttcg cagcgagtga gctggctcct gccgtcgccc
     6061 gtgccttcaa caaactgaaa acgctacgcg aaaacgcccg tagctggctg ttgaaatacc
     6121 cggaacatgc ccttaccggc ctgctgcctg cggcgctcgg caaagccggt gaagcacagg
     6181 ataacgcccg cgctgccttg cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag
     6241 aaatcgcccg acgttataac cagccggaag taaccgatgc ggtgaacgct ctgcttgcgc
     6301 tcgatccctt agataatcac ccgacaaaaa tccccactct tccggccttt tatcagccat
     6361 cgctctggac gcgcccggta ttaaaagcaa atgcccaatc actgccagat agcgccctcc
     6421 tccacctcgg tgaaatgctc cgcttccctc aggaagaggc tctgtatccg ggattattgc
     6481 aggtgaaaga cgtctgttcc gccgactcac tggcgggatt tgcctgggat ctgtttaccg
     6541 cctggcagac cgctggcgcg ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc
     6601 tcggtaacga tgacaccgcc cgcaaactga cgccattaat acgcgcctgg cctagtgaat
     6661 cccagcataa acgcgccacc gttgggttgg atattctcgc tgctatcggt agtgatatcg
     6721 cccttatgca gcttaacggc atcgcccaga aactgaaatt caaagcatta caggagcggg
     6781 caaaagaaaa aattgccgac attgccgaga gccgcgaact cacggtggcg gagcttgaag
     6841 atcggttagc accggatctc ggtctggatg ataacggttc gctgctgctg gattttggcc
     6901 cacggcagtt caccgtcagc tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg
     6961 gcagccgcct gaaagacctg cccaaaccga acaaaagcga tgatgaatcg cggtcgaacg
     7021 atgcggttaa ccgctacaaa ttgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg
     7081 tagcaaggct ggaatccgcc atgtgcctgc gccgccgctg gtcgccggaa aacttccagc
     7141 tcttcctggt tgagcatccg ctggttcgcc acttaacccg ccgtctgatt tggggcgttt
     7201 atagcgccga aaaccagcta ctggcttgct ttcgcgtagc agaagataac agctacagca
     7261 ccgctgacga tgatcttttc accctgccgg aaggcgatat ctctatcggc attcctcacg
     7321 ttctggagat atcaccgacg gatgccgccg cctttgggca gctttttgcc gactatgaac
     7381 tgttaccgcc gttccgccag ctcgaccgta acagttacgc cctgacagaa accgagcgca
     7441 atgccagcga attgacccgc tgggcaggca gaaaatgccc gagcggtcgg gtcatgggac
     7501 tggcgaataa aggttgggtg cgtggtgaac cgcaggatgg tggctgggtc ggctggatga
     7561 tcaaacctct gggccgctgg tcgttaatca tggaaatcga tgaaggcttt gcagttggta
     7621 tgtcgccagc cgaacttagc gccgagcaac tcttaagcaa gctgtggcta tgggaaggca
     7681 aagcagaaag ctatggctgg gggagtaatt caacacagga agcgcagttc tccgtactcg
     7741 atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa taaggaaaga
     7801 gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg aaatataaaa
     7861 aaggcaaaac gccgctcagt catcgtcgct ggccgggcga accagtgtcc gttatcactg
     7921 gaagtctcat ccagacattg ggagatgaat tactacaaca agcaggccag aaggaaaata
     7981 tcacctggaa ttatgataaa tgctcactgg agtggcagtc cgccatccag caggcaatca
     8041 atttgactgg cgagcataaa ccgtcaatcc cagcccttac catggcggct ctgatctgta
     8101 tcgcgcaaaa tgacagccag cagttacttg atgaaatcgt gcaacaagag gggctggaat
     8161 atgcgacgga tgtagtgata gcgcgtcagt gcattgcgcg acgttatgag agtgactcac
     8221 tggtcgtcac tttgcagtat caggacgagg actatggcta cggttatggc tccgcgacct
     8281 ataacgattt cgatctccgt ttgcgtaagc atctgtcgct ggcagaggaa agttgctggc
     8341 agcgttgtgc cgataaactc attgccgcac tgccaggaat acccaaaatt cgccgtcctt
     8401 ttattgcgct gatcctcccg gaaaaacctg aaatagccaa tgaactcgcc agtcttgaaa
     8461 gctctcggtc aagtctccat tcaaaggagt ggttaaaggt cgttgctact gataatacgg
     8521 cggtaaaaaa actcgaacga tactggggcc tggacgtatt tagcgatcgc gaagccagct
     8581 acatgtcaca agaaaaccgc ttcggctacg cggcctgcgc ctcccttttg cgcgaacaag
     8641 gactggcagc cgttccgcgc ctggcaatgt atgcccataa agaagattgc ggcagtctgc
     8701 tggtacaaat taaccatccg caagtcatcc gcaccttgct actggtggct gataaaaaca
     8761 aacccagcct gcaacgtgtg gctaaataca gtaaaaactt cccccacgcg acgctcgccg
     8821 cactggcaga actgctggcg ttaaaagaac caccagcccg cccaggttat ccaatcatcg
     8881 aagacaaaaa gctgcctgca cagcaaaaag cacgcgatga atactggcgt acgctgttac
     8941 aaacgctgat ggcatcgcag ccacaactgg cagcagaagt gatgccgtgg ttaagtactc
     9001 aagccagggc agtgctgaat agttatttat cggcaccgcc aaaaccggtt attgatagca
     9061 ccgataacag cagtctgcct gaaatgctcg tttcaccacc gtggcgtagt aagaaaaaaa
     9121 tgacagctcc acgtcttgat ttggcaccgc tcgaattaac tccgcaaatt tactggcaac
     9181 caggcgaaca agaggggctt gccgccactg agtctgcccg ttatttcagc acggaatctc
     9241 ttgcggaacg catggaacaa aaaagtggac gagttgtatt acaggaactg ggttttgggg
     9301 atgatgtatg gctgtttctg aattatatac tccccggaaa actggatgct gcacgcaatt
     9361 cactcattgt tcagtggcat tactaccagg ggcgggttga agagatcctg aatggctgga
     9421 actccccgca agcacaatta gcagaacagg cgctccgcag cggtcacata gaagcgttaa
     9481 ttaacatatg ggaaaatgac aacttctcac gttatcgtcc ggaaaagagt gtctggaacc
     9541 tgtatttatt ggcacagttg ccgcgtgaga tggctttgac cttctggctg cgtatcattg
     9601 agaaaaagca tctgttcgcg ggtgaggact attttctcag tatcctcgga ttggatgcgc
     9661 taccaggtct gctgttggct ttttcacatc gtccaaaaga aacatttccg ttaattttaa
     9721 attttggcgc aacagaactg gcgctgcctg ttgcccgcgt ctggcgacgt ttcgcggcgc
     9781 agcgtgatct ggctcgccag tggattttac aatggccgga acatacggct agtgcactta
     9841 tccctcttgt ctttaccaaa cccagcgata atagcgaagc cgcattactt gccctgcgtt
     9901 tactgtacga acagggacat ggcgaattgc tacaaaccgt ggcaaaccgc tggcagcgta
     9961 cagatgtatg gtctgccctg gagcagttgc ttaaacaggg tccaatggac atttacccgg
    10021 cacgcattcc aaaagcccct gatttctggc atccggcaat gtggtccagg cctcgactta
    10081 tcactaataa tcagcctgtt accggtgacg ctctggaaat tatcggcgaa atgctgcgct
    10141 ttacccaggg gggacgtttt tatagcgggc tggaacaact gaaaacgttc tgccagccac
    10201 aaacgctggc agcttttgcc tgggatctct tcactgcgtg gcaacaagct ggtgcccccg
    10261 caaaagacaa ctgggcattt ctggcgttaa gtctctttgg tgacgaaagc acggcacggg
    10321 atctgacgac acagatcctc gcctggccac aagaaggcaa atctgcccgt gctgtcagcg
    10381 gcctgaacat ccttaccctg atgaataatg atatggcgct gatacagctg catcatatat
    10441 cgcaacgggc taaatcccgc cccttacgtg ataacgcggc ggaatttctt caggtagtcg
    10501 cagaaaatcg cgggctaagc caggaagagc tagcggacag attagtccca accctgggcc
    10561 ttgatgatcc gcaggcgttg agttttgatt ttggtccccg gcagtttacc gttcgcttcg
    10621 atgaaaacct caacccggtt atctttaatc agcaaaacgt tcgccagaaa agcgttcccc
    10681 ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc actggcccga ctaaaagggc
    10741 taaaaaaaga tgctactcag gtgagcaaaa acctgctccc gcgtcttgaa gctgccctac
    10801 gtaccacccg acgctggtcg ctggcagatt ttcattctct gtttgttaat catcccttta
    10861 cccgtctggt tacccagcga ttaatatggg gggtttatcc ggcaaatgaa ccgcgttgtt
    10921 tactcaaagc ctttcgtgtg gccgcagagg gggagttctg caatgcgcaa gatgagccaa
    10981 ttggcctgcc tgcggacgct ctgattggca ttgcccaccc gttagaaatg acagtagaaa
    11041 tgcgcagtga atttgcacag ctttttgccg attacgaaat tatgccgcct tttcgccagt
    11101 tgtcgcgccg cacggtgctg ctcacacctg acgagtcaac cagtaacagc ctgactcgct
    11161 gggaaggtaa atccgctacc gttgggcagc ttatgggaat gcgatacaaa ggctgggagt
    11221 caggttatga ggacgcattt gtctatgacc tgggtgagta ccggctggtc cttaagtttt
    11281 cacccggttt taaccactac aatgttgata gcaaagcgct aatgagcttc cgttctcttc
    11341 gagtgcaccg tgacaataaa tccgtcactt ttgccgaact tgatgtgttt gatttgagtg
    11401 aggcgttaag cgcacctgac gtcattttcc attaaaaaca cagcgggcag tgatgcaact
    11461 gcccgttatc aacacgactt accagcgggg aaagcgatga tcgtgcaaaa agagctggtt
    11521 gctatttacg attatgagat ccctgtacct gaaaatccgt tttccttcag acttgagatc
    11581 cataaatgct ctgaattatt tacaggttcc gtctatcgac tggagcgatt ccggctacgt
    11641 ccaacatttc atcaacgtga tcgagaagat actgacccgc taataaatga tgcgttgatt
    11701 tatataagag atgagtgtat tgatgagcgg aaattacgag gtgaatcacc tgaaactgta
    11761 atagcaattt ttaatcgtga actacagaat atattcaacc aagaaataga ataatatact
    11821 ctagataatt aaagtcgcag cacatgcaac ttgaagtatg acgagtatag ctttcttaaa
    11881 gggggaaacg gtattcccct ttagatgcgt ttcaatttgc ccgttacttc aacaaatcaa
    11941 tcaaccgtaa cagagccact aataactcca gtacggcaat gagcacggtt aacttttgtg
    12001 tcagcgtgtc catccgctct ccttattaag gagcgcaaca ccagcacctt acccttacac
    12061 tgcctgttga tactggttta cgtatgttgg tacgaaacgt tgctctccgg atcagggcac
    12121 cgcctgcggc accccccgtc cggcagccag gacttaaaat tggtccgatc cggcacaacc
    12181 tcccaacggg caggattata aatctcactc actgaattgc aatggagtgt ggatatggtt
    12241 ataatttatt cgttggtacg aaatgttgca caatcagcaa gttagcgtca ctgtagtaaa
    12301 taaaagaacc tgcctcacca gcaggttttt ttattttctg cgatctactt tccagatatt
    12361 ttttgccaat agcccaacat aaatattcac atcccggtca aatggtgcgt tgaatataaa
    12421 atcgatgaat ttataaataa aaatgagtta tatgatgaat taataaaaac agtaatcatt
    12481 ttattaaagg gggaagcggt tttcccc
//
LOCUS       DAZUFB010000129        12350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZUFB010000129 DAZUFB010000000
VERSION     DAZUFB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.129"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..421)
                     /locus_tag="ACHY3M_004235"
     CDS             complement(<1..421)
                     /locus_tag="ACHY3M_004235"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4319043.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQ"
     gene            complement(565..1983)
                     /locus_tag="ACHY3M_004236"
     CDS             complement(565..1983)
                     /locus_tag="ACHY3M_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS4319044.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(1983..3530)
                     /gene="fdrA"
                     /locus_tag="ACHY3M_004237"
     CDS             complement(1983..3530)
                     /gene="fdrA"
                     /locus_tag="ACHY3M_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS4319045.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIPPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(3520..4383)
                     /locus_tag="ACHY3M_004238"
     CDS             complement(3520..4383)
                     /locus_tag="ACHY3M_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS4319046.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGQNETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(4423..5028)
                     /locus_tag="ACHY3M_004239"
     CDS             complement(4423..5028)
                     /locus_tag="ACHY3M_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFS4319047.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCFALGVDINTCDRQGKTTIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            5286..5783
                     /locus_tag="ACHY3M_004240"
     CDS             5286..5783
                     /locus_tag="ACHY3M_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS4319048.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            5875..6807
                     /locus_tag="ACHY3M_004241"
     CDS             5875..6807
                     /locus_tag="ACHY3M_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4319049.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(6849..7937)
                     /locus_tag="ACHY3M_004242"
     CDS             complement(6849..7937)
                     /locus_tag="ACHY3M_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4319050.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8079..12062)
                     /gene="ehaA"
                     /locus_tag="ACHY3M_004243"
     CDS             complement(8079..12062)
                     /gene="ehaA"
                     /locus_tag="ACHY3M_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFS4319051.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         2884 a         3346 c         2856 g         3264 t
ORIGIN      
        1 gctggctttc actgaagaat gcaccaatgg gcttggtggg atgggcaaaa cctggatcgt
       61 ttttatccac ttccacctga gttaccacgg tgacggcttt tttctcgccg tgacgcgcca
      121 gccggttatt cagcgcctgt tggatcagat agccgatgcc gccctgcgta tctgccacac
      181 agttcgccag cggcgttaag ggcagccctt cgcgctcgtg ggcaatctcc gcacggcgta
      241 aatccagccc gacctgcggc ccgttaccgt gggtcagcac aatgtcgtaa tcggaagcca
      301 gcatttccag caccgtatcg gccacggctt tcaccgcctc cgcctgatgc tcaatcgact
      361 ggctggcgtt atctttgata atgctgttgc caccaatggc aacgaccaca agctctttca
      421 tgttgttatg tccgctacag agggtgatta ggttatgacc aacgccagac tgcctgatgc
      481 gctacgctta tcagtatcta caattctcgg gccatccgta ggccggataa ggcgttcacg
      541 ccgcatccgg catgaacatg taggtcagac gccgtaacgt tcgcaccagc caagaatggc
      601 tttctcgaag caggcaaaag gtggatgcac aatgcccgcg ccaatcatgc cgacgcccgc
      661 atctttatgg gcgatggcag tgttgatcac cggcagaatg ccgctgctac ccacgcgggt
      721 gatgtcgatt gccgacggca cgcccataaa accgagcagc ggaatggtga cgttggggtt
      781 ttcaccgaga gtgatttcac gcatctggcg agagaaatcg atagcctctt ccaccgtgcc
      841 gcccaccagc gcaacgattg caggcgcggt cgccatagca aatccgccaa taccgtaggt
      901 ttcagtgatg gcgctgtcgc cgatatccag ccccgaatct tccggcttat aaccggcaaa
      961 catcgggccg atgacctgct gcgccggacc ggtaaaccat tgccccggta atccgctgac
     1021 ccgcaggccg aactcgacgc cgttacgcgc catggtggtg accacggtgc tgtattcgat
     1081 gccgtgcgcc gcatccatcg ccgctttaca catcgccatc cacgtcgggc cggagaagta
     1141 gtcgctgcta gcgacaaact caaacacttc gcgctgtttc tccacggaat aacctgcctg
     1201 aataattccc ggcgtcagcg cctgaatcag caacgtcgtc ccggcgttat tgcggttatg
     1261 gcactcatcg cccatatgca gcgcctgcgc cagcattaag cgcagatcaa tctcgccgat
     1321 aattttcatc gcgtcgcgca gcattggccc ctgcacatcg cgcatccagt tgaggcggtc
     1381 gatcacgctc tgatcgttgg ctcccatacg caaaatcttc gccatctgct cgctcatatt
     1441 ggtgtaagcg atgttgccgt aggttttgtt ttttacgatg tgcataaaca tcgaggcaga
     1501 ggtaacaccc gccatcgatc ccacacagtc gtgttcgtgg cacggcgaga aggtgatctc
     1561 cccggaggcc gccagttcag ccgcctcgtc gagatctttc gccagccctt cgaacaccaa
     1621 cgccccggtg accgcgcctt tcatcgcgcc gcacattttt tcccaggtga tcggcggccc
     1681 ggcgtgaaga atggttttcg gcgtcatgcc cggcaccacg ttaatcgcct gatcaaaacc
     1741 aatcagcacc ggatgcgact ggataatgcg ctccagcgcc tgctggttag cggcggcgat
     1801 tttgtctgcc agcggcgaat cggcaatgtt atccagcgcc tgcaccacct gcatattgcc
     1861 ctgccccggc ggcgtccagt cgagttgagt cacttctaca tgctgctttt tcaggtcatc
     1921 gctaaacatg gcgatgccga cgttaataac gttcaatggt tggctaaaca gtgactggct
     1981 catcaggctt cctccccttt gcagataaat tcacgcgcca gcaatccggt attggtgctg
     2041 ctgctcgcca gaatcactcc ggcatcaagc agcatctggc tttgttgttc taacgatggc
     2101 gtatcaagat cggtacccag cacataggcg agaatgatca actcgcgtcc ttcggcagcg
     2161 gcgatcgctt tcgcttcttt gatcgcctcg atggtggagc cgaccggatc ttcatgcgat
     2221 ccaaatccga gcacaaaatc catcacgatc accgccactt ctggatcgcg cgcctcttcg
     2281 atcaagcgac tgatgcggtt ggtggggtca atcattggat gcggcttgcc gttcgtgaag
     2341 tcgtcatcgc caaagtcgag gaaggtgtgt ttgatgctgc ggttgatatc ttgcaggcgg
     2401 aattccggat cgggcggaat gttgctgtag acatcgccat gtttttccat caccgcgaac
     2461 atggtttcgt cgcacagcgt gccgccgcag aacaggccac gaatgtattt ctgctgcggt
     2521 tgcagacgcg cacgcacatc agcaatcaac ggctggttaa gcgtatgcag gtcgagattt
     2581 tcctgtttca cgccggagag catcaccgct tttagcgccg cctctttgct gccgcgggca
     2641 aactgtagcc cctgctcatc caccggcgtt tcaacgcgac cgaggaagca gaccaccacc
     2701 ggcttgcggc aggcacgtgc gcgttccagc actttgcggg ccaccgcagg cgcgggaggt
     2761 tttgaaatca gtgcgataat ttcagtttgc ggatcgtttt ccagcatccc gatggcgtcg
     2821 agcatcatca ggccgccgat tttctcgctc aggtcgcgcc cgccggtgcc aatcagttgt
     2881 gaaacaccgc caccaaattc atgaatgcgg acgctcagct cctggctgcc ggtgccggat
     2941 gcgccaacaa taccgatatt gccgcgacgc acggcgttac caaaacagag cgccgcgccg
     3001 ttaataatcg ccgtgccgca gtccggcccc atcatcagca gccctttttc gtgggccaac
     3061 tgcttgagcg ccagttcatc ttcaactgag acgttatcgg aaaacagcat cacgttgaga
     3121 tcgttttgca gcgcctgacg cgcttcgcga gcggcaaaca gaccgttgac cgaaatcacc
     3181 gccaggttac tttccgggat atgctttttg gcgctggcaa tagtggcgta acgcgcctcg
     3241 tgcgagccgc tttgcgcttt ggtgttgaac agttcttcaa tctccaccag caactgctcg
     3301 ttgtccgcac ccgatttacc attgatgaca atcatcaggt cgccgttttt cgcctgttcc
     3361 agctccggcg tcagcagtcc taaatttttc agtacacctt tgttcatttc ggtcgccatc
     3421 gccacaaatg cctgctcgac gccgtcgagt ttatttgcac gcgtggagat agacatcagc
     3481 gagacagaat caaaataggt attcggttta atgactattt taactgacat aattccctcc
     3541 gtaggtttgg ctcagcgcac aaccatcggc catgccatac agcatgtcgc agccggaact
     3601 ggagccaata tgtttaattt tttctattgc ctgagttgcg ttcccaggga tgtcataaat
     3661 aatgttgtga ataaaacgat gaatattttc ccggcaacgt tgttgtaatg cggcttccag
     3721 cgttatagca cttaataatg tggtgttatt tctgccgcgc tgcataccga gataaaattc
     3781 ctctttgtat ttttccgacg gatgcccggg aataaataaa ataagcgcca gacctgttaa
     3841 ataatcgtcg gctgatggcg taaggccaat ccctaaccca atcatactgg cgaccgctgc
     3901 ggcgatattt tgtttttcgt ttactgcctg aataactgcc cgtcgtttaa tatgtagttc
     3961 tttatttaac gcctgataaa acggattatc gccgcgataa ttaaacaggg tttcattttg
     4021 ccccaggtgc tgatgaataa tgtcgtgcca ctgtagccag gcgataaggt gaaaattcac
     4081 agcggtcatt tgcaaggttg gggacagcca acgccgacaa cgactcatct ctatatgaag
     4141 atgttgacca accgtaatac cttgatcgtc aaactgaacc ttatcaccat gccggaacag
     4201 atcatcaaag tgagtgagtg ccaaccgaca actgtttggc gcattgtcgt actcttcaca
     4261 aagcagcgtc agcaactgct ggcgagccgg gagtgcgata tttaccgctc tggcaaagac
     4321 ctgttcaacc tgtccttgcc cccgctgcgc cagaaaatcc gcatccgcag ttagcgccca
     4381 cacacaccgt ctttctggtg tgttgatagc aagcctcccg gtttatgcac ctgcggcaat
     4441 cagtaactgc gcaatctctt caaagccccg ttcccgcgcc agttccagtg gcgttttgcc
     4501 atatttatcg gtcagatgcg ggctggcacc gtgttccagc aataactgca caatcgcctg
     4561 ctgtttaata ccaccatcat taagcacaat cgcttccagt agcggcgtcc agcctacatg
     4621 gttggtctgg ttaacgttaa tctccgtatg cgccaaaagc tcttttacaa tacttaaatg
     4681 gcctttttca caggcaggcg tcaggccgac accgccaaaa cgggttacgc aattaagatc
     4741 gggtttagcc ggtaaaataa ttcgtagtag cgttaaatca tcgttcagac agctaattaa
     4801 aaaaggattt aaacaggtat gatcttgctt attaatatcc gctccggcat caattaatgc
     4861 ctgaacgcaa gcatattgct gatataaact tgccagcgta attgtcgttt tcccctgacg
     4921 atcgcaggta ttaatatcga cacccagcgc aaagcaggtt tttactttat cgatatcacc
     4981 ctgttgtgca gccaataaat agtcggcagg tagatttttt atactcattc acattcccct
     5041 ggtttttgtg gctgttttcg atgaattgag aatagcaacg ataatctgat aaaagaaccc
     5101 gcttaaattt aattcgctgt atttcgcctt tttgttgaat gatgttcaac aatcttttcg
     5161 cagtgtgcgg tagtgcaata tttgtttctt tgacccgata ccctttcact caatcaggtt
     5221 ttatttctct atagtctgca catgtaagag tagctttcgt ccatgtctaa ccagagggga
     5281 acaagatgaa cggactcacg gcaacgggtg tcaccgtcgg tatttgtgca gggctttggc
     5341 agttagtgtc ttcacatgtc gggctctctc agggatggga attattagga accattggct
     5401 tcgtcgcctt ttgcagtttt tatgccgcag gcggcggtaa atcggggttt atcagaagtc
     5461 tggcggttaa ttattccggg atggtgtggg catttttcgc cgcgctggct gccggttggc
     5521 tggcctctgt aagtggactg tccgcgttct gggcgagcgt aattactacc gtacccttct
     5581 cggcggtagt ggtctggcaa gggcgtttct ggttactgtc ctttattccc ggtggctttc
     5641 tcggcatgac gctgtttttt gccagcggga tgaactggac ggtgacgtta ctgggttttc
     5701 tggcgggtaa ctgcgtgggg ataatttcag aatatggtgg acaaaaatta agcgaagcga
     5761 cgactaaacg cgacgggtat taaaagttat tcaaaaatga ttatctatct ttaaccgata
     5821 cctctacagt catagctgga gaaattcata gtagataatg gcgagaatgg tttaatgaac
     5881 tcaattttta ccgaagagaa tctgttggca tttactaccg ctgcgcgttt tggcagcttc
     5941 agtaaagccg cagaagagtt gggtttaacc acttccgcca ttagctacac catcaagcgt
     6001 atggagacgg ggctggatgt ggtgctgttc actcgcagta cccgcagcat tgagttaacg
     6061 gagtccgggc gttatttttt ccgcaaagct accgacctgc tgaatgattt ttatgccatc
     6121 aaacgcagta tcgatactat ttcgcagggc atagaagcgc gtgtgcgcat ttgtattaat
     6181 cagctgcttt atacgcccaa acacaccgca cgcttgttac aggtgctgaa aaagcagttt
     6241 cctacctgcc agattaccgt caccaccgaa gtgtataacg gcgtctggga tgcgattatt
     6301 aataatcagg ccaatatcgc catcggcgca cccgatacac tactggacgg cggcggtatt
     6361 gattacactg aaattggcgc gattcgctgg gcttttgcta tcgccccgga tcacccgtta
     6421 gcctttgtgc cggaacccat cgccgaaagc caactgcgtc tctaccctaa tattatggtg
     6481 gaggacaccg cgcatacgat taataaaaaa gtgggctggt tgctgcacgg gcaggagtca
     6541 attctggtgc cagatttcaa caccaaatgt cagtgccaga ttttaggtga aggaattggt
     6601 tttttgccgg attacatggt ccgtgaggcg atgacgcaat ccctgctggt tacccgacaa
     6661 atccataatc cgcgccagga ttcgcgcatg ttactggcga ctcagcattc tgctacgggt
     6721 caggtgacgc agtggattaa aaaacaattt gcgccaaatg gcatattaac ggggatctat
     6781 caggatttac tgcatcggga gaattaatcg ctgccaacct cccgatgcgc tgtgaatagc
     6841 ggtttacatc aaccacctgc tttcattacc cattcagaga taaatttatt acccggtact
     6901 ggcggagagt acaaatagcc ctgcaaaaag tgaacgcctt taccgatcat taaatccgcc
     6961 tgctcctggg tttctacccc ttccgccacg atactcagac caggcttacg cgctagttcg
     7021 acaatattgt ccacaatatg accggagatt tcgtcgacac tcgccatttg cacaaatgac
     7081 ttatcgatct taataaaatc gaccgggaac gcctgcaagt aacgataggt cgcataaccc
     7141 gtaccaaagt catccagcgc aaaggtaatg ttgtgctgat gaaggctgtc aaatatcgct
     7201 ctggcttctg gcgttaccgg aatagggtta cgttccgtta gctcgagaac cagcttgatt
     7261 ttatcgttac ctaatttctt aaccaggttc agacactctt tttcaaatcc cgctgccaaa
     7321 aaacaacccg ccgagacgtt gatgccaata tggaaattgt ccggcagcaa atgttttacc
     7381 ggcatcagaa tatccgcagt ctgtttcatc agttggcggg tcattatgac aataagaccg
     7441 gatgactccg ccagaggaat aaactgatcc ggtgggataa ttcccgtttg tggatgttcc
     7501 cagcggacaa gtacctcaca gcccgtcagt accccagtct gcgcgcagaa aaccggttgg
     7561 atccacggtt tgaattcgtg gttttccaac gccagactga tggcttcagg cgtgacgata
     7621 tggtgacaac ggttatcagg gatataaaaa ttatttttat ttcccgtgcc actgatgact
     7681 tgcggatgat attgaaaaaa gatatcccgc cagaaggtaa tatcactctg gatgcctagt
     7741 ttttcataca gctgcttctt ttgatgtgaa attgtctttg cactgcgatt tctgtactgt
     7801 gagatttctg taaccgacat tccctccatt agcagcttga gtaaaagccg ttctttttcg
     7861 gacacgatac caggcaacgt caaattcacc gttgccccga actcttccaa aaaaacactc
     7921 aaatcatgcg aagccattga acacaccttg attaaatctt gtgcgccagc taaatctgcg
     7981 agtcttcact ctgactgata aagctggctg caagaaaatg acacagagag ccgaattatc
     8041 tcccgttcgg ctctctctcc ttttactcat tattcagatc agaattgcca cttaatgcca
     8101 accattgcag aggtgtcatt atatccccta tcggcaacct gaacgcccac attcccccag
     8161 acattcaggt tggcatttag ctgtccttcc accccggttt ttatctcagc aatatttcgg
     8221 gctccatcct gagtgacgga cacgccatcc atactggtgc tgaaatcctt actgttatgc
     8281 agccagttca cttctacaaa cggctggaac tcgcgggatt taccgtcatc cattttatgg
     8341 tggctcttca gccaggtttt tacgccaagt cgggtttgaa cattgccatc accgttgcta
     8401 tgaacgaggg ttccgttgct ttcgcggtgc ttatcggctt tgactcccat ccaggtaacc
     8461 tgtgcttgcg gctgaacata ccattcatta cgcgttccct ggctgccatt aaattcagct
     8521 aatttgtgtt tgtatccagc ttccagtgaa gcggtaaatc cttttgattt ataggattca
     8581 ctttgtaagt catccccttt cactgtgtta tcaaaccagc tgtactgcgc ccaactgtcg
     8641 agatacgcgc cattacgcga ctcgtcatcg gcataccagg tggcatagag gccggtgcta
     8701 tatccgttca cactcgcttt tgcacgataa ccggttcgcg aggaaatggt tttgctgtcg
     8761 ctgttgccat atcccgccat gaccccaaca tgccagctgt cgctgccgtt ttggctccac
     8821 tgcgcgacat cgcctcccag ttgcagaaca tagcgattgc tttgggtttt cagctggccg
     8881 ctgccatcac gccatttatt atgaccacct tcatggcgca tccacatagt ggtttgtttc
     8941 tgctcacccg tcaccatgtc ggtatagtac gtattaccca gacgctcatg cagacgcgtg
     9001 gtgaacattg tattcgctgc tgcaaggttc gcaatgtagc tacccgcctc cggtcgcagg
     9061 tcattatcca cattcagatc cggacccggc gtaggtgtcg ggttagggtt cggctctgga
     9121 ttcgggtttg gctccggatt cggcatcggg tctggctccg gctgcagttc aggagaatca
     9181 ctgccgctgg tcagatacca gttaccacta tttgcccctt gtccacgcgc gagagtgtag
     9241 tcatacgccc ccgcaacgat acgaccagcc tgaacaaatt cgccctcaga cttaccgtct
     9301 acatggataa gttcgatacc gttaagggtt ttcgcacctg taccgccagc gttattcacc
     9361 gtaacggcag tcgtgccgct agtatcgccc tcgacaacca atttatcggt tactgagtca
     9421 tcgccattca gtaccgtatt cattactagt tgaccgttat taccgtggta gttccccttc
     9481 accactaaca cattgccagc ttgctgtcct gacattccgg tcatcagcgt accgttgttg
     9541 gtcagattgc cgcccagggt gtaattcaca gacgcagccg ctcttttttc agtgctatta
     9601 ttcaacgcca gggtaccgtt gttctcaacg tttgcgctac ccaatccctg cgcatgactt
     9661 accaggacat tagccgaatt agcaatgttc acattcgccg tcaggttgct gttatcgccc
     9721 gcgatgtcca gcgtgccgcc ctgaatattc agctctccgc tccccgttaa cgaaccggtt
     9781 gaagtgcccc cgttggtaac cgtcagactg ccgccattta agctcagcag tgaatcggcg
     9841 ctgccattga gcgtgcccac ggtctggctg tgaccattca tatccagttc agtctctgcc
     9901 gccagacgca gttcatgggt attacccaac acgttatcgt tattcaacaa aagcgtccca
     9961 ctgcgcagat cggtaacacc ggtatagtcg ttgtctttgt tagaaagcga tacggtctga
    10021 cccttctggc tgctgaatgc caggtcacca ctgccggtaa tctttgcgct gagatcggcg
    10081 gcattctcgc ttttaccgtt agagctcagc accagagcat cgctgtcggt agcgtgtaaa
    10141 tcaagctggg tcaggccgta accgatatac agaccgtcat tgtttacccc actcgtcaga
    10201 cgatagtcgt aagtgccgac ggccgctttt tgcccattct gctgaatatc acgttgggcg
    10261 ctgctggaga ggatttgccc atttttatcc tgcaattgca gattgcccgc atcgcccaga
    10321 acttccgcac cttgcgcatc aaccagctta atggcgctgt taccatcatc gacttcagtg
    10381 agcgataacg cagagtcaat atcacgagat actgagcgga caacgtcact gtcagaaacc
    10441 tgaatcacac cctcgccgcg caaatccagc gttttactaa cgttgaccgt cccttctgtc
    10501 atctgtgcac ctgctgtcag ggcaccaaaa tcaacggttc cgccggagaa tcccaaagtg
    10561 ctaatggagt gagcgccctt acccactgtt acctggcttc cctgacccgc agtgatcccg
    10621 cctctctgca gaaactcagc attcttttcc aggttaaatg tcgtgttctg gaacgccagt
    10681 gaaccctgga accagtctgt ataagcaaac tcatcgaagg agaattcgtg attccccatg
    10741 tttatttcaa caatacctct accttgaaga tcgttatcaa atacccaatc tttattggca
    10801 ttgatttgca gtttgccatc aacatccagg tccgcacggc cgatgttctt gtcttcagaa
    10861 ataaccgcga cagcaccaga agcgacatca atatatccgc tgaatgcata gttattgccg
    10921 gtcagtgcca ctgtcccact gtcgatactt attgtaccat tgccggaaat cttattggca
    10981 aaggtcatgc tatcgtcagc cttcaggtgt aatttcccgc ggttgttgat ctctgctgta
    11041 cctatatttt tggtgatccc taaagcgcca ttttgctcaa ttttgacttt accactaaag
    11101 gcagagttat caccggttaa gtaagtcgtg ccattgagcg cattgatata gcccaggccc
    11161 tcgctgccac tgacaatatc cgcggaaata gtcgcgtttt tatccgtgtg gtttaaggtg
    11221 attttcttat cggatgtctt tgcccacaca aactcaattt tttcggcatc aataatccct
    11281 gcagccttcg cagcacttcc ttcctgtgca cctaacgcta actcagagtt ggtgcttaaa
    11341 ctaattttcg gtgcggatat tttacctcca tctgatacga ctaatgttgt atcagaacgt
    11401 tggtcgtaat aacccaggct aatatgttca cgagcattaa cttcaccacc gttattaacc
    11461 gacaggaact gattaccgcc atatccgccg taaacgccca taaagaaggt ccctgcattt
    11521 acctgactac cttcgccgct tacattcccc tgcgtataag tcagacgact ggtgccctta
    11581 acatgaattt tgccgccatt ggagacatta aaatagcctt gatcatgtct catgcctgag
    11641 tttgactcat acaaatcatc agcatataac tcgctgccag cattggagac attcaggaac
    11701 cccgtacctt tcagcaaaaa gttaagcggg ccattgattg ttacctttcc tccggtaata
    11761 tccaacccac caccagaagc gagagtcaga cgcggacggg aagtatctgt cgtattgatt
    11821 accagattgg cattgtcagt caccgtaata cggtaatcga tgctctggtt ctctttgcca
    11881 aaatcaatag ttttatcctg gtttacgatg acatcatcgg cgaataccag agatgaatat
    11941 aaacttatcg cgctaattaa aacagcccgg gacttttttc cttttactct gctccccagc
    12001 tcagagcaga cgctccaaca gttacgggac ctgttccact ttagccgata tattttattc
    12061 atcacaatct ccagcaatac atccaaataa aaccaagatt gcataaacag caacgcatta
    12121 aatgccattc tggttactgt tcatcgcata taaaaattca aaaattttgc atagcattgg
    12181 caaccacata tctgagtatg tagttcagag ggtaataaaa tagcaagtta ttacttaaaa
    12241 cttattttaa gttaacactc ttaactttat cattacattc ctcccagtct ctaatacctg
    12301 cgcattatat gtatccagga actcaaatca actcaaaata taaacaaaag
//
LOCUS       DAZUFB010000130        11943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZUFB010000130 DAZUFB010000000
VERSION     DAZUFB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.130"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..2905)
                     /locus_tag="ACHY3M_004244"
     CDS             complement(56..2905)
                     /locus_tag="ACHY3M_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319052.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQHRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLANSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAGLATGANKLPYFTGTDTISQTDLTSVGRDILAKTSVLAV
                     IQYLDLRELGTSGEKIPLLSTANKWSARQTFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPGSGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNS
                     AGNHSHNIPVGHTGAGNGVSAGFNAALGTGTTSSAGGHAHNVYIGAHNHTIGIGAHAH
                     SVIIGPHGHTITVNATGNEENTVKNIAFNYIVRLA"
     gene            complement(2917..3351)
                     /locus_tag="ACHY3M_004245"
     CDS             complement(2917..3351)
                     /locus_tag="ACHY3M_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319053.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            complement(3430..4266)
                     /locus_tag="ACHY3M_004246"
     CDS             complement(3430..4266)
                     /locus_tag="ACHY3M_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319054.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEHADMITL
                     NEISDTEKAYCWFETSDTTSLTGVTSISAPITATPGGTIVKSTPTFDRTRADDLLLDS
                     DA"
     gene            complement(4266..5699)
                     /locus_tag="ACHY3M_004247"
     CDS             complement(4266..5699)
                     /locus_tag="ACHY3M_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFS4319055.1"
                     /translation="MSKTTPTKDSIRAEFEEIVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVRNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(5696..6052)
                     /locus_tag="ACHY3M_004248"
     CDS             complement(5696..6052)
                     /locus_tag="ACHY3M_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319056.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(6052..9417)
                     /locus_tag="ACHY3M_004249"
     CDS             complement(6052..9417)
                     /locus_tag="ACHY3M_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFS4319057.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVEAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAVAGP
                     LWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQVNT
                     QKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGVASL
                     LMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAVGKG
                     ALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLDLGG
                     LVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTISTK
                     FDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIKNPG
                     KHIKEAASNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITPELK
                     APVNSQQGTSDSKAESDAKQTNIAARVINAALDMAKDSNKTVKETANQIINANAVETG
                     NKAAQTIDAALGQSATGKEEALSAYEIDKRRFNNGKDVSLPKLNAAGYQWISDNADYF
                     DELERKYGLEKGILSAVASAESSAGQRTGNPVDKNGNKLSSALGAFQITKGTREDLGL
                     SDADAMDTRKAADGAARYLSMLMNRYNGDQGRAIAAYHAGMGHVDKGRVVAGTGEYVT
                     RVRGYQQMLNNGAVYGSKVDHSAPAIHEKIPDNAVIDQSTGLAFTPGDSPFEKGGLVD
                     KIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVDTPMPVEEAR
                     PVASNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAVNDLQQPTGR
                     MQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEEHTKDSAKGQ
                     GTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            complement(9499..10380)
                     /locus_tag="ACHY3M_004250"
     CDS             complement(9499..10380)
                     /locus_tag="ACHY3M_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HFS4319058.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(10395..11006)
                     /locus_tag="ACHY3M_004251"
     CDS             complement(10395..11006)
                     /locus_tag="ACHY3M_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319059.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(11017..11583)
                     /locus_tag="ACHY3M_004252"
     CDS             complement(11017..11583)
                     /locus_tag="ACHY3M_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319060.1"
                     /translation="MTPRQLLEDVKTRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITVQASKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(11738..>11943)
                     /locus_tag="ACHY3M_004253"
     CDS             complement(11738..>11943)
                     /locus_tag="ACHY3M_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFS4319061.1"
                     /translation="FRFITGDNVRLHVSPSLNSEVIECIGKNTIVAILDKKDRQWLYV
                     QVKSGDEFITGWITRTYTKPLKA"
BASE COUNT         2948 a         3043 c         2635 g         3317 t
ORIGIN      
        1 gccagtagat tataaatttt tatggtccgt gattgttcac tcattctgaa tgtcattatg
       61 caagcctcac aatatagtta aatgcgatgt tttttacggt gttttcttcg ttacccgtag
      121 cgttaacggt gatggtgtgt ccgtggggac caataatgac agaatgggca tgagcaccaa
      181 tgccgatagt gtggttatgg gcaccgatat atacattgtg agcatgcccg cctgcgctcg
      241 acgtggtacc agtacctaac gcagcgttaa aaccggctga tacaccattc cccgcgccag
      301 tgtgaccaac aggtatattg tgtgaatgat ttccagcgct attcgtagtt tttgtgccgt
      361 aatcaaatga actggtcgtt ttagtaccgt aatcaaacga cgatgtggtt ttcgtcccca
      421 aatccgtact ggatgcgctg gcgctgtggg tgtgcgattt aatgccgtcc tgttcctgag
      481 acaatacggc acgaccactg ccgggcttgc ccttaatcgt ccagccacgc atatcaggaa
      541 taacgcctga cggataagcc gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc
      601 cctgcatcag ggcataacca gacggaacgg tatctgatgg ccacgggatt ggtgcgccga
      661 ctgggtagct ttctggtgga agatttttcg aggtataaac ttctgcccag tcttcctcaa
      721 aaccataacc gtctcttgaa gaacggtaga acagaccacc atttctgtaa tgcgccttca
      781 tctgcaaggt cgggcaactt ccgactccgg tatagaagtt aaccagaata tagctgtcgg
      841 cagagcgaat gacattgtaa gcgcctgatt cggcattcca gggaacgccc ccatccgcat
      901 cggcatacgt atccgttgct cttctggcaa aagcagcaac atgcgcagcg gttaaagtga
      961 tatcagtaga accatcaaac gagacgccat tgatagtaca tgctgtctgc aacttggtcg
     1021 ctgtagccgc attaccagag gtgtcctgat cccctttggc attgacgccg ggaattgaat
     1081 cttttgacgt atagacctgc gcccattcag accagttggc agaatcagta tcacgccgcg
     1141 aacggatatg tacgggcgca tggtcaccgc tcgtgccact ccagccaatg aataactcac
     1201 cttcgccagc agcggtggca cctttaaggt gaagcacatt gccatagggg gaagggtagc
     1261 cattgttgta tgcctcatac agctgaatcc cggatgttcc ctgtgcattc gcctctaggg
     1321 ccgttacgcg accgcgagat accagagtat tgatattaat gtcagccgaa ccatcgaatc
     1381 tgacgccatt aatgtttatg gctgttttta atttcgtcgc ggtgtcggcg ttccctgtca
     1441 gcgccccggt gatcccaccg ttgaaagtct ggcgtgcact ccatttgtta gccgtgctca
     1501 acagggggat cttttcaccg cttgtgccaa gttctcttaa atcaaggtat tggataacag
     1561 caaggacgct tgttttggcc agaatatcgc gaccgactga cgttaagtca gtctgagaaa
     1621 tagtgtctgt accggtaaag tacggcaatt tatttgcgcc tgtagcaaga ccagcaagcg
     1681 cggttaaagt tgcatccaga ggctgtttgc ctgccagcgc atttgtcatt gttgtcgcaa
     1741 agttcgggtc attaccaagt gctgcggcaa gctcattcag ggtatcaaga gcttctggtg
     1801 atgaaccaac caatgcggat atggcagctc ttacataagc ggtcgtagca atctgcgtgt
     1861 tattcgtacc ctgtgcagcg gtaggcgcag taggtattcc cgttaatgca ggacttgcta
     1921 aaggagcttt aagagccaga gcattgttga tagttgtgct gaaattcggg tcgttattga
     1981 tcgcagcagc tatttcttta agcgtatcca gtgtgccagg tgcaccgttg ataagtgccg
     2041 ttatagctgc cttaacaaag gctgtatttg cgatctgcgt gctatttgta ccttgcgctg
     2101 ccgtcggcgc ggttggcgtt cctgtcagac tcgggctttc tattggcgct ttggtatcag
     2161 caagatcttt tacagactta acggctttag gggtagccgc cattgtttcg ctgtcgctgt
     2221 tagtttcgct actgagctga actaatccct tttgcgttgt gcttgcatcc tgcgccgtat
     2281 acttactgtt cgccagatcg taggcttttt taactgccag cgaacttgca gcaacatcac
     2341 ttctgctact ggttacagag tctgaaatat caatgccgat cgtgcggtta atacgctcgg
     2401 atgtatcaat catctcctgg gtaatggctg atactccagc agggatattc accgtacaaa
     2461 caagcagctc cccatctcct aactgatatg aatcggtata ggttctggta acaaattcag
     2521 ccgcatgaat atgtgacgcg gtattaacct gataggtatc ctctccaagg aggtatcttc
     2581 ccttcagcac aattgcatat ttcttacctg cgctaagtgc aagagaaata tccttacgat
     2641 gctgaatagt tacctggtag aattcaccaa tatccactga cgctgcgcca gcagttttat
     2701 caccatccac agaggtgatt aacaggttca tcccaccgcc aggcttaggt aagaaaccgg
     2761 cataaaatcc cgggtcaaca atccccctga attttcggtt tagcgcggct gacagatatg
     2821 gttcgtggta ttgcacatca gcaaccagag ccaacgactc gggtgatggg taagtaactg
     2881 atgtaacaac tgtaacgtca ttcatcaagc atatccttat gctgttgtcg tgtttatggc
     2941 cataactgcg gtatatgttt tgcccacata cagcgagtct tcctggacac aaataatggc
     3001 gattggcttg ttctcgttat ccagaacaac cagagtgttg aatgggtagt ttttcccttc
     3061 ctgcaactgg ctttgatcaa ggtccattcg gacagtaatt atcccacctg agtaggttgg
     3121 cacgaggttg atggtacaaa attggctggt cagttctgcc agatcgaaag cctttggcag
     3181 ttctccaatc tcataagtgc catctccttt cttagtaacc agagaactgg taccgaaaac
     3241 ggccttgctg attaaaaatc gagagccttt gttaatggac gattcagcgc gccgctgata
     3301 gtaatagtcc aacaactgac tcttatagag gtttgttgag acgtcagaca tgattttccc
     3361 taatcaatgt tgtgaagcct cattgtaaga taagtaactt gtcaccccgc cctgcggacg
     3421 gggtgattgt caggcatcgc tatccagcag caaatcatct gcgcgggtgc gatcaaacgt
     3481 aggcgtcgat ttcacaatag tgccgcccgg cgttgcagtg atcggggcgc taatcgaggt
     3541 aactccagta agcgaagttg tatccgaagt ttcaaaccag cagtacgctt tttcggtatc
     3601 agaaatctcg ttcaaagtga tcatgtcggc gtgttcattt acaacaaccg acaaatagag
     3661 cgtaagccca tcaaacacta tatgcagtgg cagtagaggc tttacgaact gattaaactt
     3721 tctgagaatt tcttctgtaa ttgcggactg atctatcgtg ccagtaatac ccattgtccg
     3781 ggccaggtcg tttatgggaa tactgatcat ccctctggaa gtcagaaaca tctcgccgaa
     3841 tgtgccgccg gtagtctcca gtgtgctttc tggtattaga accgtgccat agggatgacg
     3901 ctcaaggtcc accggtgcat atatcggatc ccataaaaca gaaataccgt taaattcgcg
     3961 gtaaattgtc tggtttatag gacgttctgt tcccttaaag tggatctcat caagccgctg
     4021 ttgtaacaac atcggaacgg aagatgagtc cgaggttctg atagtaaaga actggccaag
     4081 ttcatttgtc ctggtctcca gatcctcctt gctcatggag aaaatagact tccggttggt
     4141 aattcgctcc aaccatgggt caacaaaggt gtccatcatt gactgaacca aatcagccaa
     4201 tgatttatag agcaatgact tttgcttagc tgatgtaagc cggttattaa accaggaacg
     4261 ctgcatcact cctcctcata cgaaatatta aaggtggagt tttctgtatc cagataaacg
     4321 aaatcgtaaa agccgttgga ctcattccac tcgacaaatt ccagataaaa gtcgcggaaa
     4381 taacccagcg tttcgataaa cgcccaaacg tcttttttct taatcaggat gtacttcccg
     4441 acgcggttcg gatcaaagaa agttgagtcg cgcccaaatt ttgtttccag tgccgacttc
     4501 agctcatcgg tcacattctc aatggtcagg cttgccgata tccgtccggt gatggttatc
     4561 ttaaatggta gttttctgac ctctttatac gagaatttct tgttcaactc attcggcacc
     4621 ttcttaaagg cagccaggat catttcttca agctctgact ggcttttatt tggatgccac
     4681 cctgaaataa atatcttatt gatattccga acattataag caccatctaa tttctcttgc
     4741 tggccttcgc cccatgcctt tacccatgac agtcccggga tgttacgcac cagaaaatac
     4801 gtatagtccc cgccccatac gacctgatca tcataggcaa ggtaatattg tgcacggtta
     4861 cgtgtgatct ccgttgtttc ggcatcggta cctgcggtta taggtgtcgt tgtcttaact
     4921 gaaatcaaat tagctaaatt agccgcagaa tcgacaggcg tcaggttttg gccagcaacc
     4981 agggttatat cgccgttggt gcaccatacc ttaagcgtaa tggtcgagcc ttctggcggt
     5041 atttgcccaa ttagcccatc accgaatcga acccccaact gctcggatgg cttataaaac
     5101 tcaacgtaga cctggctttt actaccggct aaccggaaca tagtgctgga agaccactgc
     5161 gtggtcttac catcggtcgt cacgaatact tccagcttat agcagacagc agtgagagcc
     5221 tttgataaca cgacttccag aaattctttg gctgccgtaa cggtatatgt cacctcctgg
     5281 atttccaact gtgccacttc taccgtaccg gtgccgtcaa ccaacctgca tacatccata
     5341 gtcatgtaag ggtactggtc gtcagatatt aaaggcatat ttttggggat taccgctggg
     5401 gcatcttcac ttgtggcggt gatctcaatc atccccgatg acggtgttgg cttggtacca
     5461 acgtaactat tcgtttctgc cgctgccagg atagaggaac gccgcgtcgc ggtcgatata
     5521 aagccttcag ccagcgccgc atcggcatac tgaaagcacc tgtagacaat ctgggtaata
     5581 aacaatgtca gcatcgagac aaattgagag ccgacaaact tcgaccagaa tgaatctttc
     5641 tcgacaatct cttcaaactc tgcacgaata ctgtctttag tcggtgttgt tttactcata
     5701 gcaccacgtc ctgtgtgata gttatatccc tgatacgaat ggatattttc aacttatcaa
     5761 aagcatctcc ctcggctact gacaagccag aaatcggtat gtcaggtaaa tctaccgtca
     5821 gtttttgcaa cagcattgcc tcaaccgcaa tttgaacatg cgacaagttg gtcggttcgt
     5881 gtttaaactg cggtaaaaca ttgccccatg acggatcccc gtatacctca ccctgataag
     5941 tgtttagcca ctcatataaa cgagcgcccc aggcctcctc ctgggactca tacgttttta
     6001 cgccggataa ctccagcgtc agcaaaggat caatttcgtt attgttggcc atcaatcaac
     6061 tctcgcgtag tcattcatca acggatcatc aattgacagt ggtaccgtgc gcataacgcc
     6121 cggctgaggc gtgctgacct ttacgacagt tccctggcct ttcgccgagt ctttggtgtg
     6181 ctcttcaatc ctggcaagca atgaggtcat ctgcgcaaac agccgcttcg tttcaccatc
     6241 aagtgaaacg gtattatcag ccaactgcat tgtcggcttg gcaccggagc cgccaaggtc
     6301 actaataacc tgtccgtcta tctgcatacg accggttggt tgctgcaaat cgttgacggc
     6361 agtcgtcacc tgggacgtgg aggctggttc aggcgcatta tttttccgca tccccggcga
     6421 gttgcggagt ttatcgaata gtccatcaat ccccatttgt gcgccgagtt ggtcaaagta
     6481 acttgagttg ctggccaccg gacgcgcctc ttcaactggc atcggagtat caacatacac
     6541 attgccagct gctgttgcgg tccccttccc tcgtgcacgt tcttcgagcg ttccctgaac
     6601 gacttcccga cgcatacccc ggccattcat gaatttgttg accagatcgt taacaccaac
     6661 agcattgcca attttgtcta ccagaccgcc tttctcaaac gggctatcac caggggtaaa
     6721 cgccaggcca gtagactgat cgataacagc gttatcaggt atcttttcgt gaattgctgg
     6781 tgcgctatga tctaccttag agccataaac ggcaccatta ttgagcatct gctgataccc
     6841 tctgacacga gtaacatatt cgccggtacc ggcgactact ctccccttat caacatgccc
     6901 cataccagca tgataggcag ctattgcacg accctgatca ccgttataac ggttcatcag
     6961 catacttagg tatctggcgg caccatcagc tgcttttcgt gtatccatgg catcagcatc
     7021 gctgagtcca agatcctccc ttgtaccttt agtgatctga aaagccccaa gggcagatga
     7081 aagtttgttc ccgtttttgt ctactggatt tccagttctc tggcctgcac tagactctgc
     7141 ggatgcaact gctgacagaa tccctttttc aagcccatac ttacgttcaa gctcatcaaa
     7201 ataatcggca ttgtcagaaa tccattggta tccggcagca tttaattttg gcaaagaaac
     7261 atccttgcca ttgttaaatc gacgtttatc tatctcatat gcacttaatg cttcctcctt
     7321 acctgtagca gactggccca aggcagcatc aattgtttgt gctgctttat ttcctgtttc
     7381 tacggcattt gcattgataa tctgattagc agtttcttta actgttttat tgctatcttt
     7441 cgccatatcc agggccgcat ttatcacgcg ggcagcaata ttagtctgtt tggcatcgga
     7501 ttcagcttta gaatcagatg tcccctgctg gctattaacc ggagctttta actctggagt
     7561 gatttctttc gcattagcct cgccgatcgg attgggtatt tttgatacaa tcattgccgc
     7621 aggggtattt ttaacggcat caaccgctgc atctaatgct ttacctggta aatttttaac
     7681 cccatcccaa atattactag ctgcctcttt aatgtgtttc cctgggttct taatgaaatc
     7741 aattgcacta tcaattgcat cactgaaaac ctgtttcagg ttatcgacag taaagaagtc
     7801 tttgatggca tccagctttt caagcagctt attagatgta tcgctaaacc atgctgaaac
     7861 agcatcacca atctttgctg tgtaatcatc gaacttagta gaaatggtgt cgccaaggtt
     7921 agaaatatat gtttctaagt tggtaatccc actatcaatg gcctgggcaa tactttccgt
     7981 cgaaaatgat tgcaacatat tgccgatatc ctcaaatcca agtgatttga gaacatcacc
     8041 aatagcactg ctaataccag ataccagccc ccccaaatca agaacattgg ctaacgtata
     8101 agcggctttt tgctggaatg atggatcttg tcctgattta agcccaaacg ctcgacgttg
     8161 cgcttctgta tcattccaac cggttaccgc atcataaata cctccagcca ctgtgccgac
     8221 taggggaatt gcgcgtaacg cccctttacc aactgccttt aatccaagtt tacctgctgc
     8281 ccgggcagcc aaatctccgc cttcatgggc gagagtcttc ttgccaccac cgcgtagcat
     8341 tcctacaagt ttctttgccc ccagagcgcc aaaagcgagt gctccagctt ttttcagcat
     8401 gccacgcccc attaacaacg acgcgacgcc accggccccc ttccctaaca ggctaaataa
     8461 tttggacagc aagccgccct tctttttccc ggtgtttttg gctatctgat caagggcgct
     8521 gagaatcttg tcattgccct ctttaatttc gctggtctga tcctgaagtt cctgaaccgt
     8581 ccgtttttgg gtgttaacct gaacgacatc ggcactattt tgcgatttac gcctaaaaaa
     8641 accttttcta cggctgttat cgtcattgcc acgaatcaca tcggcaatag actttctggc
     8701 accattaagc gatccaccaa cttcttttga tatcccgcca agctccttcc ctgcggccca
     8761 caatggacca gcaacggcat aacctaacgc atcgacggca cgagtctctg aagggttacc
     8821 tatgccttca gctacttttg acagtttttt taataaacct gattcagcat ttagacgctc
     8881 atcatcctct ttgcgcctgg ccttttcagc acgggcatct tccgctgcgg ccttactccc
     8941 tgactttcca ataaaacgac cacgcgcatc gcgttggttt tggctttttt gcgcaccgcc
     9001 tttttgaccg aacatttcgc gagcgtgttc ggctgcttcg gtccgttgcg cctttacatc
     9061 ttctgttata gccttcttgc gtcgtttttt accctttcgc gtagttgatt tggcctgcgg
     9121 ttcctgtaga gcaacatcct cctgaactac acgagaaacg tcccctaaat taagccgttt
     9181 cattgcctca acaatagggt ccactgatgg cgcattggcc acaaagtctg gccgggaatt
     9241 ttcgattgtg cgatttaatg ccgacacact gcgagagaca ggatcaactg tagcaactcg
     9301 cccccctttc aaatcttcaa cggcttcccg aatacctgca agctcttcca gctcttttgc
     9361 gctggcggtt tcaaccgtcc ttatcacatc gtcaatgttg gcgttttttc tttccatgat
     9421 cttatcgcct accgcttcgg tttaagtttt tcttccagtt tctccaacag gaaaaacgca
     9481 taggattcag tgagcctttc agcgtcctga atcggtatac ccccatacaa aaccaggttg
     9541 gacactaagg tctgatagct tttcaatccc cacctgtgga atgaagtcgg tagcccgaaa
     9601 gggcacccac agacgggtat acgcaccctc tgtggactcc tttgtatcct ggtttgggca
     9661 tttgtgcggc gggagacgaa gacgcatttc gcctttatcg atgtagcacg gtaaaccatg
     9721 ttcgagcttt tcatgagcca gccggatgtg tgccgccagc ttcataaatt cagtatcaat
     9781 ggccatccgt ttaatcgttt cataacgacg ctcagcctga tcttcacgag taccgctaac
     9841 atcgttataa agctcacact gataagcgaa ttcccaaaaa cgcaaatcaa cgatcgcttc
     9901 tttgaattcc gcgtcgtctt caggtggcaa tgctgcacgg cgcatctcca gcatttccat
     9961 tgcccaacca tcaagcggca cgatacgcca ttgataaggt actccctcta cagacacctc
    10021 aatatcgtca atgaaaggtt ccacttccag gacctggata tcttcagcca gagcattcat
    10081 atcacaatcg taataatgct ctttaccgca atgtttacag gtgtaggtga atgtctcgac
    10141 cggtgtttca cgggagccag taaatatcca ccataacgcg gtaatccggt cctgcgccgt
    10201 ccatgtcagg ggatcatatt tcgcgggttc agccagcaag gcttttaaat acgccgttgt
    10261 ctgttgttct tgttcctccg gtgttatcga gttgaaacgc atcgcatcag caatatttgg
    10321 ctgacggaac tggatcaatt cagttggccg cgatggtagc gggaaaagag gtaaaagcat
    10381 ccttgctcct taattcaaag agaaaagcta aagcccagaa gggaagccaa agaacttgag
    10441 gattggttaa acgtgctgtg caatgcgaag gtcattggga atgacttaaa ttccgtaacc
    10501 tgatcccgcg cataggtgac atcgccggta gtgaccggga ataccgtcat ctcattttcc
    10561 agtttggtta agccggaaga cagcaaccga taaatacgca cattgagcaa atatttagat
    10621 ggtatgttcc cggtaccgtc aggattgatc acccgacttt ttgccgtctt aaaccagtcc
    10681 aaaacgaggc catcaacagt gtccctgacc atcattgtta tctgcccagg cgaacgctcc
    10741 gttggctgaa ggatattccc cccgccgatt ttaatcgttt catattcgat gctgtaatcg
    10801 tggtaggtaa tgtctttggc aaagaagtct gccccctcca gtccatcaac ttcgacagag
    10861 aactgccatc cttgcgcgaa cagcattttg ttcatgatga ttgacgtcag cttaccaact
    10921 tcccgctcac caacgccgga gccaaataat gtcgtcgtta atgccgaaga tacataagac
    10981 tttactgaag caacattaag ccccatatca gccccctcac ttcaacatgg atgagaaaag
    11041 aacaattccc gggataattg cccttgttgc gctcattttc tcttccagat ccagctttcg
    11101 ctgatacagt gtgttctcgt cggataaatt gctggcatcg agtttccccg cgatagatat
    11161 tcttcgcagg cgatcagtgt taggtattgc gattagcact tccagatagt cagaaagtaa
    11221 cccaatgatt tcaggtggca cttccccatt atccagatcc atatcacgca aattagccag
    11281 atatgacaca ttcagtgggt ataccgctcg atgggtatct tcaagctcga tattcccatc
    11341 gtaaacatcg gagtagacaa gatcgccggt gtgatctgta actgatacga gcgcaagaaa
    11401 atcagctggg caagcaagtg atttactggc ctgaacggtg atgcgtatcc gcttgatgtg
    11461 ccccgcccta tcctggtagg ttcccaatgc ttttcttagc agggattcca gtaaggcagg
    11521 ttcatccgca atcaaaggtg tgaagcgggt tttgacgtct tcgagtaatt gtcgtggtgt
    11581 cattgaaacc tcgtagaatc tggtgtgtta accgattcta cgagttgtca tttgttaccg
    11641 gtcagtctac acatttcaga tactacgtta aaacaactca agtgcttcaa gtagcaaatc
    11701 gccatcaaca cccagcccaa agatgcggag ataaaattca agccttaaga ggctttgtgt
    11761 atgttcgagt aatccatcca gtaataaact catcccctga tttaacctgc acgtaaagcc
    11821 attgacgatc tttcttatct aaaatagcaa caattgtgtt tttgccaatg cattcaatca
    11881 cttcactatt taaagaagga ctgacatgta atcttacgtt atcaccagtt ataaacctaa
    11941 agc
//
LOCUS       DAZUFB010000131        11757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZUFB010000131 DAZUFB010000000
VERSION     DAZUFB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.131"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..563)
                     /locus_tag="ACHY3M_004254"
     CDS             complement(<1..563)
                     /locus_tag="ACHY3M_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319062.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLG"
     gene            688..1788
                     /locus_tag="ACHY3M_004255"
                     /pseudo
     CDS             688..1788
                     /locus_tag="ACHY3M_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            2124..3101
                     /gene="glaH"
                     /locus_tag="ACHY3M_004256"
     CDS             2124..3101
                     /gene="glaH"
                     /locus_tag="ACHY3M_004256"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFS4319063.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3121..4389
                     /gene="lhgO"
                     /locus_tag="ACHY3M_004257"
     CDS             3121..4389
                     /gene="lhgO"
                     /locus_tag="ACHY3M_004257"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFS4319064.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4412..5860
                     /gene="gabD"
                     /locus_tag="ACHY3M_004258"
     CDS             4412..5860
                     /gene="gabD"
                     /locus_tag="ACHY3M_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4319065.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5874..7154
                     /gene="gabT"
                     /locus_tag="ACHY3M_004259"
     CDS             5874..7154
                     /gene="gabT"
                     /locus_tag="ACHY3M_004259"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFS4319066.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7465..8865
                     /gene="gabP"
                     /locus_tag="ACHY3M_004260"
     CDS             7465..8865
                     /gene="gabP"
                     /locus_tag="ACHY3M_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFS4319067.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVLALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8886..9548
                     /gene="csiR"
                     /locus_tag="ACHY3M_004261"
     CDS             8886..9548
                     /gene="csiR"
                     /locus_tag="ACHY3M_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFS4319068.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWELRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9549..9998)
                     /gene="kbp"
                     /locus_tag="ACHY3M_004262"
     CDS             complement(9549..9998)
                     /gene="kbp"
                     /locus_tag="ACHY3M_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFS4319069.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10082..10240)
                     /gene="yqaE"
                     /locus_tag="ACHY3M_004263"
     CDS             complement(10082..10240)
                     /gene="yqaE"
                     /locus_tag="ACHY3M_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS4319070.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10423..10722
                     /gene="ygaV"
                     /locus_tag="ACHY3M_004264"
     CDS             10423..10722
                     /gene="ygaV"
                     /locus_tag="ACHY3M_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4319071.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10732..11256
                     /gene="ygaP"
                     /locus_tag="ACHY3M_004265"
     CDS             10732..11256
                     /gene="ygaP"
                     /locus_tag="ACHY3M_004265"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFS4319072.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(11303..11707)
                     /gene="stpA"
                     /locus_tag="ACHY3M_004266"
     CDS             complement(11303..11707)
                     /gene="stpA"
                     /locus_tag="ACHY3M_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFS4319073.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2877 a         2914 c         3030 g         2936 t
ORIGIN      
        1 ccaagttttg gaggtttgat aggcgtttta atttcactgc gcaacgcatc taagtttgta
       61 agatcactgt ctataatttt aagatagttt aaatctaacg aaccatcagt tttatcaagc
      121 ttagttttca gccttttaac agcctccaag gaagcatcaa gaactggtgt ctgattcttc
      181 aaagcttctg aaaaactttt ttgtgttgat tttcgagctt gttgttgagc agattttctt
      241 aactcttttt cacgcttaac ttgttctaaa agctctttac ggtcatcaga acgtgaacca
      301 aaaaatctat cagcaagttc agttaacaaa ttagatataa tagttttcag ttctcttgca
      361 gcctggtttc ttatgaatcc ctctctcccc gacttatctt tcagctcttt attactggat
      421 tgagtaattc caatataacc aaaaatcctt ctattagacc aataatatcg ccctgcattc
      481 catgaacgtc tttcttctat ctggaagaaa tcattatcta ctcgaccata aggtaatact
      541 ctcaagctat ccctaaaaat cattagtcct gcatactttt tggccttaag atcaaagtgg
      601 gaatgttcac gttcagtatg tgatgtattt tgtgaaagga attcaaacgt tcctatctga
      661 agctcaaatg ggccgacccc tgcgcgcatc tctaaggatg aatgggaaaa cctccatgca
      721 ttaaaagaaa agcgtttaaa tggaatgcct aaaacaacac ccaaaagtga ccaggttatt
      781 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
      841 gatgcggcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
      901 aattataatg agcggttaca caatactaac ttatttaacc caaaatatca taaaaaagcc
      961 gttatgaatt acatggaata tctggtaact tgtcagttgg atgaacaaca aatgtcatca
     1021 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaaaacgata
     1081 aaagcctttt catttggtgg agatcttcaa acccttgcat caaaaccggg ttccacaatc
     1141 tcaagtatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     1201 ggtaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     1261 tatatagccg ctctgccgtt cagcaggcct ttagtttact gggatggcca agtattaaaa
     1321 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggcggtga ggcgtagctt
     1381 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     1441 aaagcaggaa tatggagcgc atttgagggt gtatctgcaa ctaaaaacgt tctggccttt
     1501 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     1561 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatacaaa
     1621 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     1681 ggtaaagtat ttacgcttag aggcggaact ctaaatatca atattcctgg gcgtagcgct
     1741 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     1801 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgcagtg
     1861 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     1921 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     1981 tactaatttg ttgcttttga tcacaataag aaaacaatat gtcgcttttg tgcgcatttt
     2041 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     2101 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacacaat aacgctgtcg
     2161 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     2221 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     2281 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     2341 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     2401 gtgcgctgtt gatcaatgcg gtgggtatcg atgatgtggc gcaggcagat gagatggtga
     2461 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     2521 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     2581 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     2641 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     2701 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     2761 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     2821 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     2881 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     2941 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     3001 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     3061 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     3121 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     3181 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     3241 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     3301 ctgaaggcgc agttttgcct ggcgggaaac cgcgccacca aagccttttg cgatcaaaac
     3361 gacattcgct atgacaactg cggcaagatg ctggtcgcca cgtccgatct cgaaatggaa
     3421 cggatgcgcg cgttgtggga acgcacggcg gcgaacggta tcgagcgcga gtggttaaac
     3481 gccgaggaac tgcgcgagcg cgaaccgaat atcaccgggc tcggcggcat ttttgtgccg
     3541 tccagcggca ttgtcagcta tcgcgaagtc acagcggcaa tggcaaaaat cttccaggcc
     3601 agaggcggtg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     3661 gtggtcgtcc gaacccgtca gggcggcgaa tatgaagcat cgacgctgat tagctgttcc
     3721 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggttt tattatctgc
     3781 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccatctg
     3841 atttacccca ttcccgaccc ggcaatgcca tttttgggcg tgcatctcac ccgaatgatc
     3901 gatggcagcg tcaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     3961 aagcgcgact tctcatttag cgacacgctg gagattttgg gctcgtcggg gattcgccgg
     4021 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     4081 ggctatctgc gcctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     4141 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     4201 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     4261 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     4321 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     4381 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     4441 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     4501 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg ctgatgaaac
     4561 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     4621 agaacgcgcc aacattctgc gcaactggtt caatttgatg atggagcatc aggacgattt
     4681 agcgcgcctg atgaccctcg aacagggcaa accactggcc gaagcgaaag gtgaaatcag
     4741 ctacgccgcc tcttttattg agtggtttgc cgaagaaggc aaacgcattt atggcgacac
     4801 cattcccggt catcaggccg ataaacgcct gattgttatc aagcagccga ttggcgttac
     4861 cgcagctatc acgccgtgga acttcccggc ggcgatgatt acccgtaaag ccggtccggc
     4921 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacaccgt tctctgcgct
     4981 ggcgctggcg gagctggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     5041 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgatggtac gcaaactgtc
     5101 gtttaccggt tcgaccgaaa ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     5161 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     5221 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     5281 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattgca
     5341 gcaggcggtg agcaaactgc acatcggcga cgggctggat aaaggcgtca ccatcgggcc
     5401 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     5461 aggtgcgcgt gtggtttgcg gcggtaaagc ggatgaacgc ggcggcaact tcttccagcc
     5521 gaccattctg gtggacgtgc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     5581 cctcgccccg ctgttccgct ttaaagatga agccgatgtg attgcgcaag ccaatgacac
     5641 cgagtttggc cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtggg
     5701 cgaagcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     5761 cccgttcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     5821 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaatgaaca
     5881 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     5941 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     6001 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     6061 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     6121 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     6181 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     6241 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgcgtatcac gggcgcacgc
     6301 attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     6361 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     6421 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     6481 tcgtgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     6541 agcgtttacg cgcgctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     6601 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtt gcgccggatc
     6661 tcactacctt tgcgaaatcg atcgcgggcg gcttcccgct ggcgggcgtc accgggcgcg
     6721 cggaagtgat ggatgccgtc gctccaggcg gtctgggcgg cacctatgcc ggtaacccga
     6781 ttgcctgcgt ggcggcgctg gaagtgttga aggtgtttga gcaggaaaat ctgctgcaaa
     6841 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     6901 cggagatcgg cgacgtacgc gggctgggag cgatgattgc catcgagctg tttgaagatg
     6961 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     7021 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccgc
     7081 tcaccattga agacgctcag atccgtcagg gtctggagat catcagccag tgttttgctg
     7141 aggcgaagca gtagctatgt ccgtttgtaa gagttaaacg ttgctaaggg aatgataacg
     7201 atgcagtaaa aaataataac gatgaggcga tcgacaaagt taaaaacgac tgcggactgt
     7261 cccctcgctc ctctggggag agggttaggg tgaggggtaa tcacgcggct acaccctcac
     7321 cccggccctc tccctaaggg agagggagaa aactctcccg ctttcgtttt taagattttg
     7381 ttgaccgtct caattaaacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     7441 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc
     7501 gggctgaagt cacgccacgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     7561 tttgtcggtt ccagcgtcgc catcgccgaa gcgggcccgg cggtattact ggcctatctg
     7621 ttcgccggat tactggtggt tatgattatg cggatgttgg cggaaatggc agttgccacg
     7681 cccgataccg gttcgttttc cacctatgcc gataaagcca ttggccgctg ggcgggttat
     7741 accatcggct ggctgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc
     7801 gccgctatga tcctgcactc gtgggttcca ggcattccca tctggttatt ttccctcgtc
     7861 attaccctcg ccttaactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag
     7921 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     7981 gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt gtgggatagc
     8041 ggcggcttta tgcccaacgg cttcggtgcg gtattaagcg cgatgttgat caccatgttc
     8101 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacac gccggaaaaa
     8161 catattgtcc gcgctaccaa ctcggttatc tggcgtattt ctatcttcta tttgtgctct
     8221 atttttgtcg tattggcgtt aataccgtgg aatatgccgg ggctgaaagc cgttggttct
     8281 tatcgctcgg ttctggaatt gctcaatatt ccccatgcga aattaatcat ggactgcgtg
     8341 atattacttt ccgtaaccag ctgtctgaac tcggcgctgt ataccgcgtc aaggatgctc
     8401 tactccttaa gccgtcgcgg tgatgcgccc gcgctaatgg gcaaaatcaa ccgcagtaaa
     8461 accccgtatg tggcggtgtt actctccacc ggagcggcat ttttaacggt ggtggtgaac
     8521 tattacgcac ctgcgaaagt gtttaaattc ctgatagaca gctccggcgc tatcgccctg
     8581 ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct gcgagcagaa
     8641 ggaagcgaaa ttcgcttgcg catgtggctt tatccctggc tcacctggct ggtcatcggc
     8701 tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt agaagtgatc
     8761 tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc tcgctggaaa
     8821 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg atcaggagtc
     8881 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     8941 gtaattttca accggatgaa aaattacgga tgagtttgct gacatcgcgt tatgcacttg
     9001 gcgttgggcc gttgcgggaa gcactttctc aactggtggc ggaacggctg gtcacggtgg
     9061 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
     9121 acgcccgcgc caatatggaa gcgatgctgg tcagtctggc gattgcccgc ggtggcgatg
     9181 aatgggaggc agacgttctc gcaaaagcgc atctgctcag taagctggaa gcctgtgacg
     9241 ccagcgagaa aatgcttgat gagtgggagc tgcgtcatca ggcgtttcat acagcaattg
     9301 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
     9361 cgcgctatcg atttatctgg ctgcggcgaa cggtgctttc ggtagaaatg ctggaggata
     9421 aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc gcgcgcgcca
     9481 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
     9541 gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt atccgggctt
     9601 tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc
     9661 tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagtg
     9721 gctggtgtcg ccgttttcac ctgatcatcg acactggcaa taccggaaat attcccaacc
     9781 gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac cgtcgctttg
     9841 ccgtcggcaa tttgaatgtt cactttatcg gcatcaggta taccggtttt gctcagatgc
     9901 tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc gtcccagagt
     9961 ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga
    10021 aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcggggg tgagcacagg
    10081 tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg
    10141 tcagcagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg
    10201 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
    10261 ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag cggagcctgc
    10321 cagtttcagc ttacgctttt actctcattt acgctgctga ggctggtata tgttgcattt
    10381 ttagtttaga gtttacttaa tttagaaaat acttaaataa atatgactga actcgcgcaa
    10441 ttacaggcca gtgccgaaca ggcagcggcc ttattgaaag caatgagcca tcctaaacga
    10501 ttgctgattc tgtgcatgct tagcggttcc cccggcacca gcgcgggaga gctgacgcgc
    10561 attaccggac tgagtgcctc tgcgacatca cagcatctcg ctcgtatgcg ggacgaaggg
    10621 cttatcgaca gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta
    10681 aatgccatta tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg
    10741 acaaccattt cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat
    10801 attcgtgacg ctgatgaata tcttcgtgaa catattcctg aagcagatct ggctccatta
    10861 tccgtgctgg aacagtcagg tcttccacct aaattacgtc gcgagcaaat tatattccac
    10921 tgccaggcag gtaaacgcac cagtaataac gccgataaat tagcagcgat tgccgccccc
    10981 gcagaaatat ttttactcga agatgggatt gatggctgga aaagagctgg attgccagta
    11041 gcggtaaata aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt
    11101 ttaatattaa tcggcgttgt actgggttat accgtaaata gcggtttctt cttattaagt
    11161 ggctttgttg gtgccgggtt actgtttgca ggaatcagcg gtttttgtgg aatggcaagg
    11221 ttgttagata agatgccgtg gaaccaacga acttgagaag cgacgccgga cgcgccctag
    11281 cagcgacatc cggcctcagt aattagatca ggaaatcgtc gagagattta ccttctgcca
    11341 gcgcctgagc aattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt
    11401 taacatcggt gaatttatat ttcgccggac gcggctggcg ttttttacca gcgcgtggtg
    11461 cagcagcaga gctattacct aataactctt ccgggttaat tccgtcagcc ttcatcagct
    11521 ccagccaggt gctaattttt tcctgacgct cagccagttc acgctgctgc tgttcttctt
    11581 cttcacgtct ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa
    11641 tggagaattc gcgagccatc gcacggagag tgcgaatgtt atttaaactt tgtaacatta
    11701 cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa agtatttcgt cgcgtag
//
LOCUS       DAZUFB010000132        11426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZUFB010000132 DAZUFB010000000
VERSION     DAZUFB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.132"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            252..551
                     /gene="yoaC"
                     /locus_tag="ACHY3M_004267"
     CDS             252..551
                     /gene="yoaC"
                     /locus_tag="ACHY3M_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFS4319074.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHY3M_004268"
     CDS             complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHY3M_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFS4319075.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            924..2285
                     /gene="pabB"
                     /locus_tag="ACHY3M_004269"
     CDS             924..2285
                     /gene="pabB"
                     /locus_tag="ACHY3M_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFS4319076.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2289..2867
                     /gene="nudL"
                     /locus_tag="ACHY3M_004270"
     CDS             2289..2867
                     /gene="nudL"
                     /locus_tag="ACHY3M_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFS4319077.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIIRRPQP
                     GVLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3051..4415
                     /gene="sdaA"
                     /locus_tag="ACHY3M_004271"
     CDS             3051..4415
                     /gene="sdaA"
                     /locus_tag="ACHY3M_004271"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS4319078.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4546..6144
                     /gene="pdeD"
                     /locus_tag="ACHY3M_004272"
     CDS             4546..6144
                     /gene="pdeD"
                     /locus_tag="ACHY3M_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4319079.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLLLEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6148..7704)
                     /gene="yoaE"
                     /locus_tag="ACHY3M_004273"
     CDS             complement(6148..7704)
                     /gene="yoaE"
                     /locus_tag="ACHY3M_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFS4319080.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7692..7850)
                     /gene="yoaL"
                     /locus_tag="ACHY3M_004274"
     CDS             complement(7692..7850)
                     /gene="yoaL"
                     /locus_tag="ACHY3M_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFS4319081.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8167..9138
                     /gene="manX"
                     /locus_tag="ACHY3M_004275"
     CDS             8167..9138
                     /gene="manX"
                     /locus_tag="ACHY3M_004275"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFS4319082.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9201..10001
                     /gene="manY"
                     /locus_tag="ACHY3M_004276"
     CDS             9201..10001
                     /gene="manY"
                     /locus_tag="ACHY3M_004276"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFS4319083.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10014..10865
                     /gene="manZ"
                     /locus_tag="ACHY3M_004277"
     CDS             10014..10865
                     /gene="manZ"
                     /locus_tag="ACHY3M_004277"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFS4319084.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10920..11378
                     /gene="yobD"
                     /locus_tag="ACHY3M_004278"
     CDS             10920..11378
                     /gene="yobD"
                     /locus_tag="ACHY3M_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFS4319085.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTSHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2798 a         2879 c         2877 g         2872 t
ORIGIN      
        1 tgccgttgtg ttcgccctct gactagcaac tctggttttc tttcttagct gaataatctg
       61 gttatagcgt gaggactgac cgttaaattt ctccatctta ctggataaca caccatgagc
      121 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc
      181 tttgatatta gatgcaaatt aaggtcatat atagtcttat cgataacatg gttaatttta
      241 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
      301 tatcagcgcc aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt
      361 taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag
      421 gctggaaccc tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg
      481 aacgctctgt gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
      541 cactggtctg agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat
      601 aaaaagcgtc ctttttctca caatcaaatg atcatcggct tctataacga aatcgatacg
      661 gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt
      721 tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca
      781 tcagttcctg gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac
      841 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
      901 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
      961 ggcgtcagga cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc
     1021 ttttacactc cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc
     1081 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca
     1141 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
     1201 gcccaacgca taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg
     1261 atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg
     1321 atatggcagt gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt
     1381 ctttgctgag tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct
     1441 cgccgcagga agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt
     1501 acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga
     1561 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
     1621 ttaatcaggc caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt
     1681 taagcctttc gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga
     1741 ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac
     1801 tggcgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
     1861 atatcggtcg tgttgccgta gccggttcgg taaaagtacc agagctcttc gtggtggaac
     1921 ccttccctgc cgtgcatcat ctggtcagca ctataacggc gcgactacca gaacagttac
     1981 acgccagcga tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag
     2041 tacgggctat ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca
     2101 gcattggcta tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga
     2161 ctgccattaa cggacaaata tactgctctg cgggcggtgg aattgtcgcc gatagccagg
     2221 aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc caactggaga
     2281 agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatctcgc tttcaacttt
     2341 tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca
     2401 tcatccgtcg accgcaaccg ggggtgttgc tgactcagcg ttcaattcat ctgcgtaaac
     2461 acgctggaca agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg
     2521 ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaagtta
     2581 tcggcgtgct gccgcccgtc gatagcgtaa ctggctatca ggtaacccca gtggtcggca
     2641 ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggcg gtgtttgaaa
     2701 tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg
     2761 gcgattcaca tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg
     2821 caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt
     2881 tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg
     2941 attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc
     3001 tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc
     3061 tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga
     3121 aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc
     3181 gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata
     3241 tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc
     3301 ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg
     3361 aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg
     3421 aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt
     3481 attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg
     3541 aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa
     3601 ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag
     3661 agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga
     3721 tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc
     3781 gccggatgtt agtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg
     3841 taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg
     3901 cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta
     3961 ttgaatcggt cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg
     4021 cattgtataa aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg
     4081 gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac
     4141 aggtttgcgt ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg
     4201 ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga
     4261 ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg
     4321 tcatcgaaac gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac
     4381 gcggtggtct ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac
     4441 ggatgggcga atttataccc gctttctcgt ctgctgtaat attccccact acacttccac
     4501 tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa
     4561 cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt
     4621 acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg
     4681 gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag
     4741 gcaggtagcg aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca
     4801 ttacgtaaac aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc
     4861 acactctatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct
     4921 gaacttcctg caccgcaacc acttttacgc ctgacgatcg accgtgccct gattaaaggc
     4981 agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag
     5041 atgattaaca tcgacttact gacggcaatg ctccttgagc cacaactgcc gcaaatcagt
     5101 agcgccagcc tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc
     5161 cttccgcaac ctgaagacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc
     5221 attaacgtta atggtccggg ggctacggcg ctggcatggc actatcttcc aacacaatta
     5281 ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac
     5341 cggatgagct tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc
     5401 tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc
     5461 tggaataatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa
     5521 catcatttaa ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat
     5581 gttttcccga tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc
     5641 cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa
     5701 ctgctcctcg aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc
     5761 gagctacatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca
     5821 ttttcctggc ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca
     5881 gctataggtt ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa
     5941 agactgaata ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg
     6001 cgccgtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt
     6061 gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc
     6121 acgcccatta tgccgttacg ttaatgctta ctcatcttca tcgtgcgccg gttgctcttt
     6181 aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg
     6241 ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc
     6301 cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa
     6361 ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg
     6421 cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac
     6481 cagaccttgt accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg
     6541 cagcacgccc aacaggttga tcggatcgag ggtttccggg acgataatcg ccggagacgc
     6601 cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg
     6661 tacaataccg atgatttcat ccagttcacc gcgacatacc gggaacagac tgtgcggtga
     6721 agagagcagt tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc
     6781 accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat
     6841 catgtaacgt tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc
     6901 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
     6961 agtacgcgct cgcagcggca aagtcgactg gtggcgaata aagttgcgac gcgcaatctg
     7021 gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
     7081 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
     7141 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa
     7201 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc
     7261 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
     7321 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
     7381 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat
     7441 caggtcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
     7501 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag
     7561 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
     7621 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
     7681 tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
     7741 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agagccagaa
     7801 aggtaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
     7861 actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt
     7921 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
     7981 ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
     8041 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
     8101 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
     8161 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
     8221 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
     8281 ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc
     8341 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc
     8401 cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat cccaatgctc
     8461 gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt ggcactggca
     8521 gtagaaacag gccgtgaagg cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg
     8581 caagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca
     8641 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
     8701 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
     8761 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
     8821 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
     8881 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
     8941 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
     9001 aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt
     9061 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
     9121 agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt
     9181 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
     9241 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
     9301 tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg
     9361 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg
     9421 ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
     9481 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc
     9541 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
     9601 cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
     9661 cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
     9721 ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt
     9781 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
     9841 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
     9901 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
     9961 aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    10021 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc
    10081 tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    10141 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    10201 aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc
    10261 tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg
    10321 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    10381 tctggggaac cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca
    10441 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    10501 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    10561 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    10621 tggttaacaa gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga
    10681 cgggcaaaga acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg
    10741 taccactgct gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt
    10801 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    10861 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    10921 tgacaatcac ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct
    10981 acgatcagtt catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    11041 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    11101 acgtcacgag tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg
    11161 gtttttatat attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct
    11221 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    11281 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc
    11341 tggaaaaaat ttataagctt ctcgtttcaa ctcaataagt tatgaattta gccaaagcta
    11401 tgtttagtgt atttttaata atcaga
//
LOCUS       DAZUFB010000133        10945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZUFB010000133 DAZUFB010000000
VERSION     DAZUFB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.133"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..885
                     /gene="adiY"
                     /locus_tag="ACHY3M_004279"
     CDS             124..885
                     /gene="adiY"
                     /locus_tag="ACHY3M_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFS4319086.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1022..2359
                     /gene="adiC"
                     /locus_tag="ACHY3M_004280"
     CDS             1022..2359
                     /gene="adiC"
                     /locus_tag="ACHY3M_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFS4319087.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2463..4106
                     /gene="eptA"
                     /locus_tag="ACHY3M_004281"
     CDS             2463..4106
                     /gene="eptA"
                     /locus_tag="ACHY3M_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFS4319088.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAISVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4103..4771
                     /gene="basR"
                     /locus_tag="ACHY3M_004282"
     CDS             4103..4771
                     /gene="basR"
                     /locus_tag="ACHY3M_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFS4319089.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4772..5872
                     /gene="pmrB"
                     /locus_tag="ACHY3M_004283"
     CDS             4772..5872
                     /gene="pmrB"
                     /locus_tag="ACHY3M_004283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFS4319090.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5848..5937)
                     /gene="pmrR"
                     /locus_tag="ACHY3M_004284"
     CDS             complement(5848..5937)
                     /gene="pmrR"
                     /locus_tag="ACHY3M_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFS4319091.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6049..7551)
                     /gene="proP"
                     /locus_tag="ACHY3M_004285"
     CDS             complement(6049..7551)
                     /gene="proP"
                     /locus_tag="ACHY3M_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFS4319092.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7815..8693)
                     /gene="rdcB"
                     /locus_tag="ACHY3M_004286"
     CDS             complement(7815..8693)
                     /gene="rdcB"
                     /locus_tag="ACHY3M_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFS4319093.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8690..10918)
                     /gene="crfC"
                     /locus_tag="ACHY3M_004287"
     CDS             complement(8690..10918)
                     /gene="crfC"
                     /locus_tag="ACHY3M_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFS4319094.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2739 a         2851 c         2698 g         2657 t
ORIGIN      
        1 aatattagta aaactcgtta tttttatgca tgtttatatt catcatacaa ttatataacc
       61 atttcccggt atcgctttgc tttagcgaga actggtcttt tatttgcact caggagtaca
      121 tgtatgagga tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg
      181 ataagggtga atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat
      241 tgtgaaaata atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt
      301 cacgacatca tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta
      361 tggcaacgta gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc
      421 gttgccgcgc aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc
      481 gcattattat tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg
      541 atgtatatgt tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt
      601 cacaaagact ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta
      661 aagaaaaagt tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg
      721 cgttatgccg taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc
      781 tgcggctaca acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg
      841 ccgctgcatt atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg
      901 aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa
      961 tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat
     1021 tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc
     1081 ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct ctactggcgg
     1141 gattgccatt tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata
     1201 cgccaaaatg tcgttcctcg atccaagtcc tggtggttct tacgcgtacg cccgccgctg
     1261 ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg
     1321 caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa
     1381 agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa
     1441 cattgtcggt ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat
     1501 ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc
     1561 ggcatggaac gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac
     1621 gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc
     1681 gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt
     1741 actttctacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc
     1801 gccattcggt gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt
     1861 ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac
     1921 ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc
     1981 gggcacgcca gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag
     2041 cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt
     2101 tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggtc acggtcactt
     2161 tggtaaagca cgcccggcat atctggctgt tactaccatt gccttcctct actgcatttg
     2221 ggccgtggtg gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtaat
     2281 caccgccatg tatgcgctga attacaaccg gctgcataaa aacccgtatc ccttagatgt
     2341 accaataagc aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac
     2401 ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg
     2461 tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg
     2521 ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc
     2581 tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca
     2641 gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct
     2701 gcctgtttat tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca
     2761 tcgaccgctc gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga
     2821 tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct
     2881 gctggataaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca
     2941 atattctggt ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg
     3001 cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg
     3061 ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg
     3121 gtgaagacgc ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc
     3181 tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa
     3241 ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca
     3301 cggcaacggc aatttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag
     3361 aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg
     3421 tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga
     3481 acgtcaccgc gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc
     3541 tgttccacgg gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac
     3601 acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat
     3661 ttaccccaac ctgcgacacc aatgagatcc agacctgtag caaagagcaa ctggtgaaca
     3721 cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga
     3781 aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt
     3841 taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa
     3901 aacaggtgcc gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc
     3961 agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca
     4021 cgctattggg attaactggc gttgagacga agtattacca ggctgcggat gatattctgc
     4081 aaacttgcag gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt
     4141 gcagggattg attctggcgg cgcaaactga aggctacgcg tgcgatggcg tgacaaccgc
     4201 gcggatggcg gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg
     4261 gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct
     4321 gccagtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgctg ggctggatgt
     4381 cggtgccgat gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg
     4441 cgccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac
     4501 gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa
     4561 agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat
     4621 tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca
     4681 tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg
     4741 ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcattttc tgcgccgacc
     4801 aatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct
     4861 gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc
     4921 gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc
     4981 cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc
     5041 ggtgcgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcactgc
     5101 cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc
     5161 ggcgttaaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac
     5221 cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact
     5281 gctggcgaaa acgcatcaca ttgatgtagc gccgttagtg gcacgacttg atcagatgat
     5341 ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa
     5401 ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac
     5461 catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt
     5521 tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg
     5581 ttacagcccg caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat
     5641 ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc
     5701 gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg
     5761 cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac
     5821 gcgggcctgg gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct
     5881 gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc
     5941 actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag
     6001 ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat
     6061 tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc
     6121 aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc
     6181 ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat
     6241 ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat
     6301 cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag
     6361 caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc
     6421 tggcaaggta gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa
     6481 cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa
     6541 cacaaacagg gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag
     6601 caagcccatc accggctgga caaacagcat accgatcata atggcgataa taatcagcac
     6661 cccgtggtct tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa
     6721 catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca
     6781 gtgtttggtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc
     6841 gccctgttcc agtttatcga catgctgctg gaacgccgga gtttcttcca gcgcatggcg
     6901 caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca
     6961 gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accactacgc cagcacccag
     7021 cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc
     7081 aggtgagtat tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc
     7141 ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga
     7201 cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat
     7261 cttctggcga ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat
     7321 cagaaaggga acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc
     7381 cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca
     7441 ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat
     7501 aatggtgaca tcacgaaggg taattggttt tacttttttc cttttcagca tagctttcct
     7561 cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg
     7621 agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg
     7681 gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta
     7741 ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg
     7801 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgagcat tgcccccgcc
     7861 agacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc
     7921 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
     7981 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
     8041 cgcgtggtcg gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa
     8101 tcacccagca acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc
     8161 ccccaacgca actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
     8221 gccccagcac gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg
     8281 cctcgttgcg ttaacgcctt ttccttccag cggcaacggg ctgaaaacca ctcatacaag
     8341 gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc
     8401 agcagcgcca gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac
     8461 gccagttttt ccaccaatgt cagacgcagg ctaagcggcg catttaacat gccgccgttc
     8521 gtccatgtct gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc
     8581 tgctgatggg cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg
     8641 ggataaaccg actccagcac gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt
     8701 ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc
     8761 cgtgagtcat cacagtccgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
     8821 gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga
     8881 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
     8941 ctttagccat ttgttggcaa taaagatcga cttctgcgct taaagctcgt tgcaacacac
     9001 tgtaatcaac cgtctctgtc ggggatttct catttccccg tccccagtcg ggctgattca
     9061 accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt
     9121 cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actgcgccgt
     9181 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
     9241 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg
     9301 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
     9361 agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaacgcg gctaactgtg
     9421 cattcaccgc atcttgctgt tgacgcagaa agtggttggc ggaggccaag gccagctcga
     9481 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
     9541 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
     9601 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc
     9661 gcagagcata caacgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg
     9721 cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca
     9781 gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt
     9841 gttgctctgg cggtggtaac ttaccgttgt tggctaactc atggcgcgcc cgattcgcca
     9901 ggtagcccca catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca
     9961 gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat
    10021 cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac
    10081 ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg
    10141 aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg
    10201 gcccgggggt atccagtaac gtcaactgac cgggataact ttccagcccc gccagatgga
    10261 caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat
    10321 cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa
    10381 taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa
    10441 gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac
    10501 gcaggcgctg ttgtaatttt tgaattaaac ggtcgattgg cgcgacatgt gaaaaatgca
    10561 gtaccggttc tttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc
    10621 gattacgatt aggcaaaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg
    10681 ctttcatggt accgacaatc gccagcacca tttccagtcg ggaaatttta cgcaactcat
    10741 tattcagcat cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg
    10801 gcgcatcgtc tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag
    10861 aaagctgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag
    10921 aaaattcttc cttaaagcaa atttt
//
LOCUS       DAZUFB010000134        10926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZUFB010000134 DAZUFB010000000
VERSION     DAZUFB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.134"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(256..1938)
                     /gene="glcA"
                     /locus_tag="ACHY3M_004288"
     CDS             complement(256..1938)
                     /gene="glcA"
                     /locus_tag="ACHY3M_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFS4319095.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2294..4465)
                     /gene="glcB"
                     /locus_tag="ACHY3M_004289"
     CDS             complement(2294..4465)
                     /gene="glcB"
                     /locus_tag="ACHY3M_004289"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFS4319096.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4487..4891)
                     /locus_tag="ACHY3M_004290"
     CDS             complement(4487..4891)
                     /locus_tag="ACHY3M_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFS4319097.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4896..6119)
                     /gene="glcF"
                     /locus_tag="ACHY3M_004291"
     CDS             complement(4896..6119)
                     /gene="glcF"
                     /locus_tag="ACHY3M_004291"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFS4319098.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6130..7182)
                     /gene="glcE"
                     /locus_tag="ACHY3M_004292"
     CDS             complement(6130..7182)
                     /gene="glcE"
                     /locus_tag="ACHY3M_004292"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFS4319099.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7182..8681)
                     /gene="glcD"
                     /locus_tag="ACHY3M_004293"
     CDS             complement(7182..8681)
                     /gene="glcD"
                     /locus_tag="ACHY3M_004293"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFS4319100.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8932..9696
                     /gene="glcC"
                     /locus_tag="ACHY3M_004294"
     CDS             8932..9696
                     /gene="glcC"
                     /locus_tag="ACHY3M_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFS4319101.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9703..10845)
                     /gene="yghO"
                     /locus_tag="ACHY3M_004295"
     CDS             complement(9703..10845)
                     /gene="yghO"
                     /locus_tag="ACHY3M_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFS4319102.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2575 a         3057 c         2747 g         2547 t
ORIGIN      
        1 ggtagtttta acgattaaga ttgcaaaagc aacagataga acggtgtttg ctcatcaagc
       61 gaacataatt aatacagact tgttttgcgt gataaaaaaa atagatgttc tcacgctctt
      121 aattatttat caggttatct gaacacaaaa cattattgcc gcatggataa taaagcgaga
      181 aataattttc aattaataag acagaataat gtaaatgaag ccggatgata ttaacgatca
      241 tccggcatta ttgatttacg agaccaacat tccagtaaac acatacgcct gcaacaacgt
      301 gataacgccg ataacgctgg caaaaatcag actgtgcttc acggtgtagc ggaatagttc
      361 agattctcgg cccaccatgc ccgtcgcggc acaggccacg gcgatagatt gcggggagat
      421 catcttgcca gttacgccgc cgctggtatt tgctgccacc agcagagtgt cagagacgtt
      481 gatttgctgc gccgtggtcg attgcagtga accaaacagg gcgtttgaag aagtgtccga
      541 gccggtaagg aataccccca gccagccgag gaacggtgag aaaaacggga acatcacgcc
      601 tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg gaatagttgg tgacgaaggc
      661 gaacgccagc accatgccaa tcgacaatat cggccacttc agactaatta gcgtttcggc
      721 aaagacgcca atccctttct tgatgcccac gccgaggatg aagatagaga taatcgccgc
      781 aataaaaata gcggtgccgc cagccgagag agggtcgaat ttaaacaccg catccattgg
      841 cgttggttgg gcgacaatgg gtgccgcttt caacacttgt tgatgcaaat gagggatctg
      901 gaaattaatc accagtgaat aaaacgcgcc gcccggagca aataacgctt taaacggctt
      961 catggtccag atggtgacca gcaccgttaa gattaaaaac ggtgaccacg cacgaatgat
     1021 ttgccccaga ctatattctg aaggcacggg accgccagaa gatggcttat ttaccaccat
     1081 cgcacctgcg gattgcccca tgctgattgc cgtttcggta tttttcggcc gccagacttt
     1141 aaggaataaa gcgagtgaga cgatactcac cagcgccgaa gtaatatccg gcagttccgg
     1201 accaatatag ttagaggtaa agaactgagt gacagcgaag cttcccccag caaccagcgc
     1261 cgctggccac gtctctttca cccctttcca gccgtccatc attgctacca gccagaacgg
     1321 cacaagaacc gacaggaacg gtaactgacg tcccgccatt gcgccaatgt ggaacggatc
     1381 gattcccgtt acctgaccgg cgaccagaat cggcacgccc aacgcaccaa acgccactgg
     1441 cgcagtgttg gcaatcagac acagccccgc cgcgtataac ggtttgaagc ccaggcccac
     1501 cagcagcgca ccggtaatcg ccaccggcgc accaaagcca gccgctcctt ccagcaacgc
     1561 accaaaggag aaaccaatca gtaacacctg caaacgctga tcgtcggtga tggagataac
     1621 cgagctgcgg ataatatcga actgaccgct ggcaacggtt aatttataga ggaacaccgc
     1681 cgcgacaata atccacgcaa ttggccataa tccataaata aagccatagc ctgcagcagc
     1741 aaatgccata tcaatcggca ttttaaaggc gaatatcgca atcaggatag ataagataag
     1801 ggttattgct ccagcgacat gtcctttcag acgtaatacc gcgagtgcaa cgaagaagaa
     1861 tattatcggg atcagggcga ccagagcgga tagccccagt cctcccatcg gcatatacat
     1921 ttgggtccag gtaaccatat tgttcagtct cttattatct ttatatgctt gatatactta
     1981 aggttgtaat aagcaaaaga ggactgaact gtaaaatata ggcgttatac tttacagcaa
     2041 cagtacgccg ctaacgcaat tgctacctct ggcataacaa gtatatcggg taagggtttc
     2101 tgttctgcac acgcaggcgc agagtatcgt taagatgtcc atattgttgt tataggcccg
     2161 ctagtaatgc gctacgggta tttaatattg ttaaaccctg ataatcgctc cggttatttc
     2221 cgggataaat gtactaccgc agttactatc atagccccga caataaaact tgccggggct
     2281 ttttttgacg ctattaatga ctttcttttt cgcgtaaacg ccaggcgtgt aataacggtt
     2341 cggtatagcc gtttggctgt ttcacgccga ggaagattaa atcgctggca gctttaaaag
     2401 cacacgagtt agcgaaattc cccgccatcg gacgataagc cggatcgtca gcgttttgct
     2461 gatcaaccac tttcgccata ttctccagcg acgcctgcac ctgttctttg gtcagaatac
     2521 cgtgacgtaa ccagttggcg atatgctggc tggagatacg cagcgttgcg cggtcttcca
     2581 tcagcgccac attgtgaata tccggcactt ttgaacaacc aatcccctgc tccacccagc
     2641 gcaccacgta ccccagaatc ccctgcacgt tgttatccag ctcttgttgg atctcttgcg
     2701 ccgaccagtt agcgttttca gcaaccggaa tagtcagcag atcgtccagc agcggttcaa
     2761 attcagcatt gaactcagtc tgggcaatgt tggcttgcac gctctgtacg ttggtttggt
     2821 ggtagtgcat cgcatggagc gtagcggcag ttggcgacgg aacccaggct gtgtttgccc
     2881 cggcacgcag ttggtcgccc ttctggctgt acatgtctgc catcaggtcc ggcattgccc
     2941 acatgccttt accgatttgc gctttaccgc gcagcccaca gaacaggccg gaaagcacgt
     3001 tattacgctc gtaggctttg atccacggcg tcgatttcat ctgattttta cgcagcatcg
     3061 ggccagcttc catcaccgaa tgcatttcat cgccggtacg gtcgaggaaa ccggtattga
     3121 tgaacgccac gcggttgcgt gcctgagcga tacagctacg caagttcagc gaggtccgac
     3181 gttcttcatc cataatgccc attttcaggg tattcggtgc cataccgagc attgtctcaa
     3241 tgcgggtaaa cagtttgttg gcgaacgcca cttcctgcgg accgtgcatt ttcggtttca
     3301 caatatagac gctgccagtg cgcgagtttt tctgcacttt taaatcatag agggcaatcg
     3361 cgccagtcat gacgccatca agaatgcctt ccgggatttc attgccttcg ctgtcccaaa
     3421 tcacaggaat ggtcatcaaa tgacccacgt tgcggataaa cagcagcgag cgtccgtgca
     3481 gagaaatttc agagccatcg gcggcggtgt actgacgatc gtcattcagt ttacgcacga
     3541 tttgtcgacc gtttttctcc attttctctt ggagagtccc ctgcatcagg cccagcagat
     3601 tgcggtacag caggatttta tcttccgcat caaccgccgc gaccgaatct tcgcagtcga
     3661 gaatggtact gattgcagct tcgacgataa catcgttgat gtgcgccgca tcgtctttcc
     3721 caatccgtcc gttggcatca atttgcagtt caatatgcag gccgttattt ttcagcaaaa
     3781 tgcaggtcgg cgcagcggca tcgccacggt aaccgacaaa ctgcgccggg gtacgtaacg
     3841 tggtttcttt accatttttc aactggatgc gtaattgttt atcgaccacc tgaaacgcca
     3901 ccacatcctg atagctgccg ttttccagcg gtagagattc atcgaggaaa cgccgaaccc
     3961 aggcgataac ctgctcaccg cgctgcggat cgtagccgct gaccatcgcc ccttcctgcg
     4021 ggatgatgtc gctgccgtat aacgcatcgt acagtgagcc ccagcgagcg ttcgccgcgt
     4081 tcagcgcgta gcgggcgttc attgccggaa ccaccagctg tggccctgcc tgactggtga
     4141 tttcgctgtc aatgcccgtg gtttccaccg tcacgcactc cggttgcggc accaggtagc
     4201 ccagttcacg caggaaagat ttataggccg ctttatcttt taccggcccc ggattgctgc
     4261 gatgccactc atcaagcgct gcctgaatgc gatcgcgttc tgccagcaac tgacgatttt
     4321 ctggtgccag atcgtgaacg atctcatcaa aattgcgcca gaacgccgca gcgtccagcc
     4381 ctgttcccgg taaaacttct tcatccacaa aacgtttaaa attggcgtca atgcgtaaac
     4441 ggccctgggt tatggtttga ctcattgttt ctctcctcgt tttcgcttat ttcgccaaca
     4501 ccgctgctgc cgctttcgcg acctgcgcat cctgtgctcc ggttaaacca gaaacgccca
     4561 cggcaccaat aatttgccca tcaacaacaa ccggtacgcc gccttccagc gacgtcagta
     4621 acggcgcagt cacgaacgcg gtacgtccgt tgttcaccat ctcttcatag cccttagttt
     4681 cacgacgccc cagcgcggcg gtacgcgctt tctcctggga gatgtaagcc gcaatcggcg
     4741 cgcaatcgtc catgcgactt aacgccagca gatgaccgcc gtcatcggca acagcaatgg
     4801 aaaccgacca gttatttttc tgcgcctctt cctgacctgc ggcaataatt gcactcgcca
     4861 tttgctggct aagaatgact ttagttttca tttttttatt ccttttcaag ggcttgttct
     4921 acaatttcaa tccagtgacg cactgaggta cgaccggcgc tcgccagatg cgtctggcaa
     4981 ccaatgttgg cggtgacgat catttccggt ttgccgcttt ccagcgcatt cattttgtta
     5041 tcccgcagct ggcgtgccag atcgggatgc gttaacgcat atgttcccgc tgaaccgcag
     5101 cacagatggc tgtcgggaac gtccgttaag gtaaatccaa gacgaagcaa cactttttcc
     5161 acttcgccgt tcagcttttg cgcatgttgt agggtacacg ggcagtggaa ggccagtttt
     5221 ttatcgccgc gaactgccag tttttccagc ggttcctcgc gcagaagctc gactaaatcg
     5281 accgccagtt cactgacttg acgcgcttta tcggcatata acgcatcgtt tttcagcatc
     5341 tgcccgtact ctttgacaaa cgcgccgcaa ccgctggcgg tttgcaaaat tgcctcggca
     5401 cctgcttcaa tcgcgggcca ccaggcatca atattattgc gcgcccgtgc cagccctttc
     5461 tcctgcgcat taagatgata gtccaccgcg ccacaacagc ctgcttcgtt agctgacatg
     5521 acgctgatcc ccagacgatc cagcactcgc gcagttgccg cgttggtgtt gggcgaaagc
     5581 gtaggctggg cgcagccttc cagcattaaa acccgacgct tatggcgcag cggcggacgc
     5641 ggtttagctt tcaccgtttc agcaggcagt tttgctctga cctgttccgg taaaaacggt
     5701 cgcagcacca gccctacctg cgtcagcgca cggaagaccg ccggacgcgg cactacctgg
     5761 cgcaatcctt cgcgcagcat tcgctccggc agtgggcgtt tcactttctg ctcgacaata
     5821 tcacgcccga tatccagcaa attgtgatag cgcacaccag aaggacaggt ggtttcacaa
     5881 ttgcggcaag tgaggcagcg atcgagatgc tcctgtgttt taagcgtgac ttcgttgcct
     5941 tccagcacct gtttaatcag atagatgcgc ccgcgcggcc cgtccagttc atcgcccaga
     6001 agctgatagg ttgggcaggt tgcggtacaa aatccgcagt gaacacaggc gcgcaggatg
     6061 ctgtcggctt ccagcgcgcg cgcgttctgc cgcatctctt cagttaattg ggtttgcata
     6121 gcctgctcct caaagttccg cgtacatgcg accggggtta aacacgccgc aagggtcgag
     6181 ctgctgttta agctgctggt gatagcggaa taaaggagcc gatagcgggg caaagccacc
     6241 atctccggca ctaaagcggg tcgcatgacc gccagcgttg cgggcgatgc gatggatttg
     6301 attgtcctcg gctgtcgatt tcagccagcg taacgctccg ccccagtcga tcagttgctc
     6361 gccgggtaaa tccatcatcg gcgcatcgct gggtaatgaa atgcgccata aggtacctgg
     6421 taacgagaag aacggcagtt gttgttcacg caattgctgc cagaactgac ccgcaacctc
     6481 ttcgcccccc agcagttcac gcgccgcttt taccgatcct tcgccgccct caaggcggat
     6541 ccacaacgca ttgtcgaagt aacataagcc actaatgggt agcggctgga gttgccactg
     6601 ggcgatttcg ttaatggctt cttgcaggct gatttcccga cgcaggctca gggaggcgcg
     6661 cggtcgcggt aacactttca tcgagatttc agtgagcacg ccaagacaac cgtagcttcc
     6721 ggccattaac cgtgagagat cgtatccggc aacgtttttc atcacttcgc caccaaaacg
     6781 cagatgtttt ccagcgccgg taatgatgcg cgtgccgagg acaaaatcgc ggaccgaacc
     6841 gctccacggg cgacgcggcc ccgccagccc gcaggcgacc atcccgcccc aggtggcttc
     6901 ttcaccataa tgcggcggct cgcaggggag catttgcccg gcgctctcca gcgccgcttc
     6961 aatcgccacc agcggcgttc cggcacgcgc ggttatcacc agctcggtcg ggtcgtaatt
     7021 aacaatgccg cgatgacaac gcacatccag cgtttgcccg gtgacagggc gacctaaaaa
     7081 ggctttgcta ttgctgccct gaatcaccag cggcgtttta tcgctaattg cctgattcac
     7141 ctgctccagc agcgcctggc tgtaatcaca ctcgcgtagc atcagaaacg ctccagttca
     7201 gggaaaggta aatgaccgtg atgcacatgc atggcaccaa attcagcaca gcggtgtagc
     7261 gtgggaatgt ttttcccagg gttcagcaaa ccatcggggt caaacgccgc cttgaccgca
     7321 tggaaggtcg tgatttcatc gctgttgaac tgggcgcaca tttgattaat tttttctcgc
     7381 ccgatgccat gttccccact gatgctgccg ccaacttcaa cgcagagttc gaggatcttc
     7441 ccacccagct cttccgcgcg ggcaaattca ccgggttcgt tggcatcgaa aaggattaac
     7501 gggtgcatgt tgccatctcc ggcatgaaag acgttggcaa cacgtaaatc atattgctgc
     7561 gataaacggg caatgccttc cagtacgcca ggcagggcgc gacgcgggat ggtgccatcc
     7621 atgcagtagt aatccgggga gatacgtcct accgccggga acgcattttt gcgaccggcc
     7681 cagaaacgta cgcgctctgc ttcgtcctgt gccagacgga cgtcagtcgc gcccgctttc
     7741 aacaagatgt cgttaacccg ctcgcagtct tcctgtacgt cagactccac gccgtccagc
     7801 tcgcataaca aaatcgcttc ggcgtcgacg ggataaccgg catgaataaa atcttccgcc
     7861 gcgcggatcg acaggttatc catcatctcc agcccgccgg ggataatgcc attggcgatg
     7921 atgtcaccaa ctgcaagtcc ggctttttct accgagtcaa agctggctaa cagaacccgc
     7981 gccacgggcg gcttcggcag cagttttacc gtcacttcgg tggtcacgcc gagcatacct
     8041 tccgatccgg tgaacagcgc cagcaggtca aaaccaggtg aatccagcgc gtccgatccc
     8101 agcgtcagcg cctcgccgtc cagcgtttgc acttcaattt tcagcaggtt atgtacggtc
     8161 agaccatatt tcaggcagtg gacgccgccg gcattttctg ccacgttgcc gccaatggaa
     8221 caggcgattt gtgaggaagg gtccggtgcg tagtagagat tatgcggtgc aacggcctgg
     8281 gagatcgcca ggttacgcac gcctggctgc acgcgcgcgc ggcgaccaac ggggttaatg
     8341 tcgaggatct ctttaaagcg cgccatcacc aacaacacac ctttttccag cggcagcgcg
     8401 ccaccagaaa gcccggtgcc tgcaccacgg gtcaccaccg gtacacgcag gcgatggcag
     8461 acagcaagaa tcgctgtcac ctgttccatt tgcttaggca gaacaaccag taatggacgc
     8521 gtgcgatacg cgctcaaccc gtcacactcg taaggaatga tctcctcatc ggtatgcagg
     8581 atctcaagtc cagggacatg ctcacgcagt gccatcagta ccgatgtgcg gtcgacatcg
     8641 ggtaaagcgc catcaagacg ctcttcgtac aagatgctca tgagtaggct tcgctttgtt
     8701 gtgttgtgtg gcagctgatt tttgcgcgct gcttctgtga acagttatta agcgggcttt
     8761 tcgttttcgt ctatctcttt agctaccggt cagaccattt tttttccagc tctgtgacct
     8821 tgccttggtt aactcaatgt taaattgatg taacataatc acttacgtga tgtgcgtgtt
     8881 ttgcgagtta agaacagaaa aattggtcct acctgtgcac gaggtccggg aatgaaagat
     8941 gaacgtcgcc ctatttgcga agtggttgca gagagtatcg aacggttaat tatcgacggc
     9001 gtactgaagg tcggtcagcc acttccctcg gaacgtcgac tgtgtgaaaa gctcggcttc
     9061 tcacgctccg cactgcgtga agggctgacc gtgctgcgcg ggcgcgggat tattgaaacg
     9121 gcgcagggtc gcgattctcg tgtcgcacgg cttaatcggg tgcaggacac cagcccgctg
     9181 atccatctgt tcagtacgca gccgcgaacg ctgtacgatc tgctcgacgt tcgcgcatta
     9241 ctggagggcg aatcggcaag gctggcggca acgctgggaa cgcaggctga ttttgttgtg
     9301 ataacccgct gttatgaaaa aatgctcgcc gccagtgaga acaacaaaga gatttcgctg
     9361 atcgaacatg cgcagttgga tcacgctttc catctcgcca tttgtcaggc ttctcacaat
     9421 caggtgctgg tgtttacgct gcaatcattg accgatctga tgtttaattc agtgtttgcc
     9481 agcgtaaata atctctacca tcgaccacag caaaaaaagc agatcgatcg ccagcatgcg
     9541 aggatctaca acgcggtgtt gcagcggctg ccgcacgtcg cccagcgcgc agcacgcgat
     9601 catgtgcgga ccgtgaaaaa gaatctccac gatatcgagc tggaaggcca ccatttgatt
     9661 cgctcggcgg tgccgctgga gatgaacctg agttagctgg tattaaatct gcttttcata
     9721 caatcggtaa cgcttgtacg gctccgcccc aatgcgttcc agcatgttat tcatgcctgt
     9781 attggtttcg aggatccatg acatctccag cgcatcgatc ttccggcggg caaacggatc
     9841 gcgtaaggct tcaatcaata acagcgcaat caccgggccg atgcggctga actgatactc
     9901 gtcgcgcacg cccatcagcg gtactcgcgc agttcgcaca ccgctgactt tcaaacgcca
     9961 cagcaatttt gcccagccga agggaaagag cgatccgttc agatcggcaa tcgcctcgtt
    10021 gatgttcggc aagccgacaa taaacgcgca gggtgcagaa tcaatctcag cgatatagat
    10081 catatcgtcc ggcaccagat atttaagttg atcgcccatg gtcgcgaatt catgttcggt
    10141 aaacggcaca aatccccagt tgtgctgcca gccagagttg aaaatctcac gcaggatctg
    10201 catttcttcg gcaaaccgct gacgattgat gcagcgaatg gtcacctttt tgcgcacctg
    10261 atccatcagt ttttttagcg ccggagagaa agtgagatcg gttcgctgca tccaccacgc
    10321 cagtaaatca atgcctttgt gataacccag ttgttcaata tgcgcggcat accacggttt
    10381 gccgtgtggc atcatcgcac agggtggtgt gtcaaaacct tcaatcagta atccgctttc
    10441 ctgattgata ttcaggctga aaggaccgct gatcttactt gcaccttgtg acttcaacca
    10501 cgcttccgct gcgccaaaca acgcggcaaa aacctgcgga tcatcaatgg cgtcaatcat
    10561 gccgaaatga ccggtatctt caccgtaacg ctcacggtgc aaggtatcta tttgcgcggt
    10621 aatacgccca actatctgcc ccgctttttt tgccacccac gcctgccaga tgatatggtc
    10681 cgtccccgga tttttcgcag acaaatgctc gttgcgttct atgaataaag ggggtatcca
    10741 gtttggatcg tcgggataaa gtgatgacgg aaaagcgata aatgccttaa ggtcattttt
    10801 attaattact ttttcaattt taatcatcag cagatcgcat tccatatctc tgtggataac
    10861 aaataattaa cggtgaaaaa caggttgcaa cagcaacgaa cgacacctta ataatcaacc
    10921 caatga
//
LOCUS       DAZUFB010000135        10822 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZUFB010000135 DAZUFB010000000
VERSION     DAZUFB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.135"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..1353)
                     /gene="cyuA"
                     /locus_tag="ACHY3M_004296"
     CDS             complement(43..1353)
                     /gene="cyuA"
                     /locus_tag="ACHY3M_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFS4319103.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(1381..2712)
                     /locus_tag="ACHY3M_004297"
     CDS             complement(1381..2712)
                     /locus_tag="ACHY3M_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4319104.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(2987..4351)
                     /gene="tdcG"
                     /locus_tag="ACHY3M_004298"
     CDS             complement(2987..4351)
                     /gene="tdcG"
                     /locus_tag="ACHY3M_004298"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS4319105.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(4423..4812)
                     /locus_tag="ACHY3M_004299"
     CDS             complement(4423..4812)
                     /locus_tag="ACHY3M_004299"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFS4319106.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(4826..7120)
                     /gene="tdcE"
                     /locus_tag="ACHY3M_004300"
     CDS             complement(4826..7120)
                     /gene="tdcE"
                     /locus_tag="ACHY3M_004300"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFS4319107.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(7154..8362)
                     /gene="tdcD"
                     /locus_tag="ACHY3M_004301"
     CDS             complement(7154..8362)
                     /gene="tdcD"
                     /locus_tag="ACHY3M_004301"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFS4319108.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(8388..9719)
                     /gene="tdcC"
                     /locus_tag="ACHY3M_004302"
     CDS             complement(8388..9719)
                     /gene="tdcC"
                     /locus_tag="ACHY3M_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFS4319109.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKALM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(9741..10730)
                     /gene="tdcB"
                     /locus_tag="ACHY3M_004303"
     CDS             complement(9741..10730)
                     /gene="tdcB"
                     /locus_tag="ACHY3M_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFS4319110.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         2785 a         2960 c         2553 g         2524 t
ORIGIN      
        1 tgcgccagca tcgcatccgg cacgataccc aaaacctggc gtttatctgg ccttgctcgc
       61 cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta acgcacacag
      121 gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg tcacggcggt
      181 atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg aaaccttcat
      241 cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga tcatactgct
      301 gatcgccatc gagatggttt cataacgccc atccaccagc catgccatcc cggcggcggc
      361 ccccattgct gcggtcgttg cggcacacag cgcagacaaa cgcggtaact ggttatggat
      421 gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agccgttcat catccgctcc
      481 gaagtgttct gctaccacca ccacaggcat tgttgcggtg atcccctggt tacccgagcc
      541 ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt
      601 acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc actgtttttc
      661 cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg ataacgcaca
      721 atttagcttc gcggaatcga gaataaagcg gatcgccgca aacgggactt cattgacgaa
      781 cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact cttgctcgcc
      841 ctctgccaca cacgcctgct gggtaaacac cacaccattg tgcgtctcaa tatgcacaat
      901 gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc agactttggc
      961 gcgtgagaag aggatttcat tgcaaggttc ctggatctta acggagactt tccccgccgc
     1021 cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca cttccagccc
     1081 ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc ccaccattcc
     1141 cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc
     1201 tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca gcgccagtga
     1261 aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga ggatgtaacg
     1321 ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa
     1381 tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa ccagtgacga taatcaggta
     1441 cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc acgccgggat
     1501 caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca acaccggtgc
     1561 gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac
     1621 aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca ttacgatccc
     1681 ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga cagcaaagat
     1741 attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc
     1801 ggcaatcgcc agcgcggaaa tattctgctc ataggctttt accgcttcgt catgtcccat
     1861 cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat
     1921 attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc gcgagcgata
     1981 agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg taaacggcag
     2041 cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga cgttatacag
     2101 atgccacatt cccaccatcg acacgcccag cgccgcgacc accagcagct tggtcagcac
     2161 catgccggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg ccaccagaat
     2221 gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg tcacgccgaa
     2281 ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga acatccaaat
     2341 caccagcatt acgaaataga gcgcacctaa caggatgccc cagtttttac ctaaataacc
     2401 gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat taataaacaa
     2461 ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa atacccacaa
     2521 tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat
     2581 actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc gccactcgct
     2641 ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc ctttattcga
     2701 tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt ttccgagccg
     2761 catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa agaaatgata
     2821 cgcgcgaggt cagagaaaat cttcacaatc aagactttga aataacggag atggataaga
     2881 attttctact aaattaatcg caagagaata ttcattctct atttgcggca gatcacaaaa
     2941 aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag ccgcagacca
     3001 ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata tctttgccgg
     3061 tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag gtgcggcgca
     3121 tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt tcaatgcacg
     3181 ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc tccatcgcga
     3241 tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt aaccctgccg
     3301 ccgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg ccagagatgg
     3361 aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag cgggcaattg
     3421 accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc ggaataatgc
     3481 cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt tcttcactaa
     3541 ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta gagatgttat
     3601 cgctggaaac cagctgacga cgcagcgcta cggcacgacg cggcacattg agcggaccag
     3661 gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact tgccagatac
     3721 gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca
     3781 ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct gctgagtgga
     3841 aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa
     3901 taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt cccttccagg
     3961 cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag ataatgttct
     4021 ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc ccgctgcgcg
     4081 ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga ctgtttcctg
     4141 ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt gacaacgacc
     4201 catacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt tccagccgat
     4261 caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag ggaccaatcc
     4321 caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc cgttcagtga
     4381 agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac tacgtactgc
     4441 gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca cacagctccg
     4501 ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat tgatggtggc
     4561 aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca cgctcagccc
     4621 ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc
     4681 agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga agaccatgct
     4741 gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt gcgtttcgat
     4801 aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat
     4861 cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag acacggattg
     4921 tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc cgattcatta
     4981 cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa tacccatcca
     5041 gcaggccgac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc gcaggaacga
     5101 ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg
     5161 tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg cccggcgcga
     5221 acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg tacaccacgt
     5281 tagaagtgat agtcagaatc gactgggtag ggacggcgtt gcgataggtt ggcagcgctt
     5341 taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt
     5401 tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt
     5461 cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc gccaccgaca
     5521 ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc gccatcagcg
     5581 aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact
     5641 gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg taatccagca
     5701 cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc tcgtccaccc
     5761 cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca aagaactgca
     5821 tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga
     5881 agtcagtacg catcagatcg tcattttcat actgcaagga agaggtgacg atcgacacct
     5941 gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg gtcaggttag
     6001 gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca
     6061 ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag attgggtcgc
     6121 cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg atcttcatga
     6181 tgaagtgatc gatcagttcc tgtgcctgct gctcattgag tacgccagct ttaaagtcgc
     6241 gctcaatgta gatatcgagg aacgatgccg tgcggcccag cgacatcgcg ccgccatttt
     6301 gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc tgcgcattct
     6361 gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc tgcaacagcg
     6421 catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga tcctcgcctt
     6481 tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgtacc agataactga
     6541 tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacggcca tagccatctg
     6601 gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag taaacatcaa
     6661 atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat tcactgtcca
     6721 tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg aacgggtgta
     6781 gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc ggctggttaa
     6841 tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg
     6901 cgtgggttgc gttttcgata cggatacctt ccattacttt ttcccacaat tccgtggtgg
     6961 caggcgtcgc ttcggcgagg aaagattcat cgccttcata cggtgtatag ttatgttgaa
     7021 taaaatcgcg gacattaatt tcgtttttcc agtccgtacc tttaaagcca agccatgcgt
     7081 cggcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat ctctctacaa
     7141 tacttcaact aaattatgca aattctgcgg gcgcgttaac tttgcctaaa tgaatggcat
     7201 ccaaagcaat cattttttct tcgttagtcg gaataacagc gcaaatgaca cgcgcatttt
     7261 cactggaaac aattcgctca ccacaggagt tagagcgatt attcatttct gtatcaatct
     7321 ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaagctt gaattctctc
     7381 ctattccgcc ggtgaatata attccatcca ggcgacgtaa tgaagctgcg tgtccggcaa
     7441 tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt
     7501 catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata cctaataatc
     7561 ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt tggctggcga
     7621 cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc aagccttcca
     7681 gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt gcgaaccgcg cagattgacg
     7741 cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga ttcagcagcg
     7801 aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac
     7861 ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga gccatcgtct
     7921 ggtggaaact ggtatcaaat accgctacct gagttacgcc cggaaataat tgctgcgccg
     7981 attcaatacc acttaaattg gcgtaattat gcaggggtgc cagtggagaa acgcgacgga
     8041 tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg ccgccgtgag
     8101 cgatgcggtg gccaattaag gccacactgt catttaaatt ccgtttttcc agttcaaatg
     8161 caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc tctcccccat
     8221 ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac attaatactt
     8281 cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag ttaataacca
     8341 aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta aaacagtttg
     8401 tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt atccagacga
     8461 ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa caggcacagc
     8521 agggatgcga taatcggtgc gcccatggct tcaataaggt cgaggatgtt cgggttggcg
     8581 taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgatagtgtt cagtttaccc
     8641 agcgacactt tagttttgtc gcctttataa ccgaacttca gaatcagacc attcaagcct
     8701 tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag tgcgatgatg
     8761 gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat ggacgcaaag
     8821 tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc catgttggcc
     8881 ggagacagcg taaacaggca gctaaaggca aagaacatca ccactgcaac catcagcatg
     8941 ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc gaagtctttc
     9001 tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa gttaaaggag
     9061 aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg accggttaac
     9121 gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg gatcagcgac
     9181 agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat cactttaacc
     9241 atcagagcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa cagcgccaca
     9301 aagccgcgat tcagcggtgc aaatccgagc tggttttccc agaacgtcat aaaggtattg
     9361 gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta caggaacgtg
     9421 ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat gttgccggaa
     9481 gggttagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg
     9541 tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc gcggataggg
     9601 aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca tgtggtatct
     9661 gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga agtactcata
     9721 tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa
     9781 agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat atattggtct
     9841 aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt gacgacttta
     9901 ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac cagcacgatg
     9961 tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga gacatcacaa
    10021 ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg gaaagaagcc
    10081 gccatgccgt gaacgttttc agactgtacg ccaataacac gaatggtcgg gttaatagat
    10141 ttaattgcca ccgcaatacc agcaattaaa ccgccaccac caattggcac aatcacgtta
    10201 tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc agcaatcact
    10261 ttcggatcat cgtaaggtgg gataaaaata cggccttcca tttcgacaat ttcgctcact
    10321 ttagcgatag tgtcgttgaa attatcacca tgcagaacga cttctgcgga gtagtcgcac
    10381 gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc gtcgataccc
    10441 agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca ggccactaca
    10501 cctttacgtt tttccgcatc agtcagtgaa cttaatttat taaatgcgcc acgaatttta
    10561 aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt gcaacgttca
    10621 ctaaaatagt ttgagcgagg catgcctgtt ttataaattc gcccagccag tcgttgtttc
    10681 gcttcaataa tgtcatcaat agcaaccggc agatcgtatg taatatgcat tataaaacct
    10741 cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca gacaggtgga
    10801 ttatttatgt ttaaataaaa ac
//
LOCUS       DAZUFB010000136        10391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZUFB010000136 DAZUFB010000000
VERSION     DAZUFB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.136"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..1852
                     /gene="ydeN"
                     /locus_tag="ACHY3M_004304"
     CDS             170..1852
                     /gene="ydeN"
                     /locus_tag="ACHY3M_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4319111.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1904..3061
                     /gene="ydeM"
                     /locus_tag="ACHY3M_004305"
     CDS             1904..3061
                     /gene="ydeM"
                     /locus_tag="ACHY3M_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS4319112.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3027..3149)
                     /locus_tag="ACHY3M_004306"
     CDS             complement(3027..3149)
                     /locus_tag="ACHY3M_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319113.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            3352..5037
                     /gene="yddA"
                     /locus_tag="ACHY3M_004307"
     CDS             3352..5037
                     /gene="yddA"
                     /locus_tag="ACHY3M_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4319114.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            5075..7447
                     /gene="yddB"
                     /locus_tag="ACHY3M_004308"
     CDS             5075..7447
                     /gene="yddB"
                     /locus_tag="ACHY3M_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS4319115.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7504..10287
                     /gene="pqqL"
                     /locus_tag="ACHY3M_004309"
     CDS             7504..10287
                     /gene="pqqL"
                     /locus_tag="ACHY3M_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4319116.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNESGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3058 a         2284 c         2273 g         2776 t
ORIGIN      
        1 cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct
       61 ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc
      121 tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc
      181 attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc
      241 atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt
      301 ctcagacttt acgccgactg aatacagtac caaaggaaag ccaaatatta tcgtactaac
      361 catggatgat cttggttatg gacaacttcc ttttgataag ggatcttttg acccaaaaac
      421 aatggaaaat cgtgaagttg tcgataccta caaaataggg atagataaag ccattgaagc
      481 tgcacaaaaa tcaacgccga cgctcctttc attaatggat gaaggcgtac ggtttactaa
      541 cggctatgtg gcacacggtg tttccggccc ctcccgtgcc gcaataatga ccggtcgagc
      601 tcccgcccgc tttggtgtct attccaatac cgatgctcag gatggtattc cgctaacaga
      661 aactttcttg cctgaattat tccagaatca tggttattac actgcagcag taggtaaatg
      721 gcacttgtca aaaatcagta atgtgccggt accggaagat aaacaaacgc gtgactatca
      781 tgacaacttc accacatttt ctgcggaaga atggcaacct caaaaccgtg gctttgatta
      841 ctttatggga ttccacgctg caggaacggc atattacaac tccccttcac tgttcaaaaa
      901 tcgtgaacgt gtccccgcaa aaggttatat cagcgatcag ttaaccgatg aggcaattgg
      961 cgttgttgat cgtgccaaaa cacttgacca gccttttatg ctttacctgg cttataatgc
     1021 tccgcacctg ccaaatgata atcctgcacc ggatcaatat cagaagcaat ttaataccgg
     1081 tagtcaaaca gcagataact actacgcttc cgtttattct gttgatcagg gtgtaaaacg
     1141 cattctcgaa caactgaaga aaaacggaca gtatgacaat acaattattc tctttacctc
     1201 cgataatggt gcggttatcg atggtcctct gccgctgaac ggggcgcaaa aaggctataa
     1261 gagtcagacc tatcctggcg gtactcacac cccaatgttt atgtggtgga aaggaaaact
     1321 tcaacccggt aattatgaca agctgatttc cgcaatggat ttctacccga cagctcttga
     1381 tgcagccgat atcagcattc caaaagacct taagctggat ggcgtttcct tgctgccctg
     1441 gttgcaagat aagaaacaag gcgagccaca taaaaatctg acctggataa cctcttattc
     1501 tcactggttt gacgaggaaa atattccatt ctgggataat taccacaaat ttgttcgcca
     1561 tcagtcagac gattacccgc ataaccccaa cactgaggac ttaagccaat tctcttatac
     1621 ggtgagaaat aacgattatt cgcttgtcta tacagtagaa aacaatcagt taggtctcta
     1681 caaactgacg gatctacagc aaaaagataa ccttgccgcc gccaatccgc aggtcgttaa
     1741 agagatgcaa ggcgtggtaa gagagtttat cgacagcagc cagccaccgc ttagcgaagt
     1801 aaatcaggag aagtttaaca atatcaagaa agcactaagc gaagcgaaat aactaaacct
     1861 tcatgcggcg gaattttccg ccgccttatt gagcgagata gcgatgcacg ttacagccaa
     1921 gccctccagt tttcaatgta atctcaaatg tgattactgt ttttaccttg aaaaagagtc
     1981 gcagtttact catgaaaaat ggatggatga cagcactctg aaagagttca tcaaacaata
     2041 tatcgcagcg tctggcaatc aggtctattt tacctggcaa ggcggtgaac ccactctggc
     2101 tggcctggat tttttccgta aagttattca ctaccaacaa cgctatgcag gccaaaaacg
     2161 tatttttaat gcattacaaa cgaatggcat tttattgaat aatgaatggt gtgcttttct
     2221 caaagaacat gagtttctgg ttggtatctc gatcgatggc ccccaggagc tacatgaccg
     2281 ttacagacgc agtaattcag gtaacggtac ttttgcaaaa gtgatagcag ccatcgagcg
     2341 tctgaaatca tatcaagttg agtttaatac gttaaccgtc attaataacg ttaatgtcca
     2401 ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata tgcaatttat
     2461 cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg agaacacatt
     2521 ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt caaccatttt
     2581 tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg aaagctttgt
     2641 cagccgtttt ttggggaatg ggcataccag ttgtattttc caggagtcct gcaaggataa
     2701 tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct atccacagta
     2761 caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac aactgacagc
     2821 gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta tctgcaatgg
     2881 cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt atttttgcga
     2941 aggttataaa aacctttttt caaccatggt accttatatg aacgccatgg tggagttagc
     3001 taagaacaga gtaccgcttt atcacattat ggatgttgca aaacaaatgg agaataatta
     3061 ataacacgcc cttttagctc cattcttagc ctccttctta tgaccttaac aaggcacccg
     3121 acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa tcctggctta
     3181 cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa ccataactaa
     3241 tgaaaatgaa atatttcttt atatttcata ttgttaagtg atacttaaat aaatttcatc
     3301 agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct aatgataacc
     3361 attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa gcccttttgg
     3421 ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc catgatcctt
     3481 ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt caatgcgcta
     3541 agccagaaag aaaccgataa gctttggcag cttgtccttt ggttccctgc cctgctgggg
     3601 atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac tattcgctgg
     3661 cgcgaatggt taacagatta ctaccttaat cggtggtttg cagataaaaa ctactacttc
     3721 acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat cgctgaagac
     3781 attctcttac tgatcagcaa aacgctcagc ctttcatttg gcttcatcca gtcacttagc
     3841 atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc attcactgtt
     3901 ggtggaacag aatggaatat ccaggggtat atggtctata ccgttgtgct cattgtgatc
     3961 ggtggaactt tatttaccca taaggttggt aaacgtattc gtccgcttaa tgtggaaaaa
     4021 caacgtagcg aagcgacatt tcgaacaaat cttgtgcagc ataataagca ggcagaactg
     4081 attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgagcgacaa ttttcatacc
     4141 attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta ctggcagaat
     4201 atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca gtttattagc
     4261 gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct ggtatcgaac
     4321 aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc ggttatcgat
     4381 cgtttgtatg agttccatca actcaccgaa cagcgcccta cgaataaacc taaaaattgc
     4441 caacatgcgg tacaagtggc tgacgcgagt attcgtactc ctgataataa gatcatatta
     4501 gagaacctga actttcatgt ttcgccaggc aaatggctat tactgaaagg ttactctggt
     4561 gcgggaaaaa ccacactgct taaaacatta tctcactgct ggccgtggtt taaaggtgat
     4621 atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa aaccggctta
     4681 ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc gttgagcgaa
     4741 gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca cgatcgctgg
     4801 ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt aattttacga
     4861 cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca agaggctatc
     4921 cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat ggttacacat
     4981 caatccggtg tctggaacct ggcagatgat atttgtgaca ttagcgcagt tttataaaaa
     5041 caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta ttcctggcgt
     5101 cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt acatgcatgt
     5161 ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca acacgcatta
     5221 caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg atctgctgag
     5281 aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc agggagatat
     5341 tcgcccggag aaaatctcta ttcacggtgc gtcgccctat cagaatgcct atttgattga
     5401 cggtattagt gccactaata acttgaaccc agcgaatgag tccgatgcca gtagtgcaac
     5461 caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg acaatgtgac
     5521 gctttatgac agtgttgtgc cggttgaatt tggtcgcttc aatggcgggg taattgatgc
     5581 aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc gcactacgcg
     5641 ttcggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta atcaaggctc
     5701 ttcaggaagt acttattact ccccagattt taaaaagaac ttttatacct tgtcgtttaa
     5761 tcaggaactc gctgataact tcggcgttac cgccggttta tcgcgccgcc agtctgatat
     5821 cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac agtataaaaa
     5881 cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct ttacccacga
     5941 tttaacctta aaatataccg gctccagccg tgattataat accagcacct tcccgcagtc
     6001 tgatcgcgaa atgggtaata aatcctacgg tctggcatgg gatatggata cgcagctcgc
     6061 atgggccaaa ctgcgtacca ccgttggttg ggatcatatt agtgattata cccgtcacga
     6121 tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag ggcgttgtac
     6181 ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca aaacacgcct
     6241 ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg gcgcggaata
     6301 catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg tgattaatgc
     6361 tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc tgggaattga
     6421 caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt taatgcccgg
     6481 tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc gctttatgac
     6541 ggaatgggat atttttgctg atcaaacctc aatgattaca gcaggttata accgttacta
     6601 tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga cggaatcggt
     6661 atcaggcaat aaaaccctga cgcgttatca ggatttgaaa acgccttata acgatgaact
     6721 ggcaatggga ttgcagcaga aaatcggtaa gaacgttatt gcgcgcgcaa actatgttta
     6781 ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga ctaaaaccac
     6841 cattactgaa tataacaacg acggcaaaac aaaaacgcat tcgttcaacc tcagttttga
     6901 actggccgaa cccctgcata tcagccaggt agatattaac ccgcaaattg tctttagcta
     6961 tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca ataccggtga
     7021 taaccaggtg gtttataacg gtaatctggt ttcttacgat agcgttccag tggcagattt
     7081 taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga gcgggttagt
     7141 gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc tgggtaagac
     7201 aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg aaaaactgga
     7261 tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga aacaacaaaa
     7321 cctgacgatc agtgccgata ttctcaatgt attggatagc aaaaccgctg ttgatacaac
     7381 gaataccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg tcagcatgaa
     7441 attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg catggagata
     7501 attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct ccccgggcgg
     7561 ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact gggcaatggc
     7621 ttgcgatata tgatttatcc gcatgctcat ccaaaagatc aggtaaattt atggctgcaa
     7681 attcataccg gttcattgca ggaagaagac aatgagagcg gcgtggctca ttttgtagaa
     7741 catatgatgt ttaacggcac aaaaacatgg ccgggtaata aagtcatcga aacatttgag
     7801 tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata ccagctatga cgaaacggtg
     7861 tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat ggcaatcttc
     7921 agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga acgtggcgta
     7981 attactgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca ggcgcgccgc
     8041 cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct gatggatact
     8101 gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta tcaaccaaat
     8161 aatatgacct ttatcgtggt tggcgatatc gacagtaaag aagcgctggc gctgataaag
     8221 gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa atcgcgtctg gccgacaaaa
     8281 gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt gaacggcatc
     8341 gcactctatt atcgcctgcc aatggtacaa gtgcacgatg agcaaagctt tatcgaacaa
     8401 gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg catacagtcg
     8461 ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc acccgattat
     8521 cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc tgcgaatgca
     8581 ttaatggcag agttggcaac tattgatcag catggttttt ctgctgaaga actcgatgat
     8641 gttaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc tgaacgtgat
     8701 ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc gttcttgtcg
     8761 ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt gcaaagtctg
     8821 gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca aatggtaaac
     8881 aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct ggaaaaagag
     8941 tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc gttaacagta
     9001 gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa tctgacatca
     9061 ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga agagcaaaag
     9121 ctacaaatta ctgccgtatc gaataaaggc gatttaagtt tccctgcgca gcaaaaatca
     9181 cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg ttggtgagct ctcctcttcc
     9241 agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt cagtggcatg
     9301 aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt tcagttgatt
     9361 aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct acaaaatgct
     9421 caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc ccagcagatg
     9481 tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca gattgtacag
     9541 tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc agcggatatc
     9601 acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg tggcgttaat tacgcgttac
     9661 ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt aactcgcgcg
     9721 acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca ggtttcacag
     9781 tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc gctcgatgct
     9841 tttaacgtcg cactggcaaa agatctacgt gttaatattc gtgaacaggc atctggagca
     9901 tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag tcatttgctg
     9961 gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttagcgaa tgaagtgatg
    10021 gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatacca gcaaaacgtt
    10081 cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac tattgtaaat
    10141 agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt gttgaaacaa
    10201 atgacggtag agaatgttaa cactgccgtt aaacaatatc tttctcatcc ggtaaatact
    10261 tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt tggcggggat
    10321 tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa ataataatca
    10381 agtactaata g
//
LOCUS       DAZUFB010000137        10276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZUFB010000137 DAZUFB010000000
VERSION     DAZUFB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.137"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(23..98)
                     /locus_tag="ACHY3M_004310"
     tRNA            complement(23..98)
                     /locus_tag="ACHY3M_004310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Lys,seq:ttt)
     gene            complement(245..320)
                     /locus_tag="ACHY3M_004311"
     tRNA            complement(245..320)
                     /locus_tag="ACHY3M_004311"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Lys,seq:ttt)
     gene            complement(324..399)
                     /locus_tag="ACHY3M_004312"
     tRNA            complement(324..399)
                     /locus_tag="ACHY3M_004312"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Val,seq:tac)
     gene            complement(549..624)
                     /locus_tag="ACHY3M_004313"
     tRNA            complement(549..624)
                     /locus_tag="ACHY3M_004313"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Lys,seq:ttt)
     gene            complement(627..702)
                     /locus_tag="ACHY3M_004314"
     tRNA            complement(627..702)
                     /locus_tag="ACHY3M_004314"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(667..669),aa:Val,seq:tac)
     gene            complement(838..913)
                     /locus_tag="ACHY3M_004315"
     tRNA            complement(838..913)
                     /locus_tag="ACHY3M_004315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Lys,seq:ttt)
     gene            complement(1078..1869)
                     /gene="cpoB"
                     /locus_tag="ACHY3M_004316"
     CDS             complement(1078..1869)
                     /gene="cpoB"
                     /locus_tag="ACHY3M_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFS4319117.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1879..2400)
                     /gene="pal"
                     /locus_tag="ACHY3M_004317"
     CDS             complement(1879..2400)
                     /gene="pal"
                     /locus_tag="ACHY3M_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFS4319118.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2435..3727)
                     /gene="tolB"
                     /locus_tag="ACHY3M_004318"
     CDS             complement(2435..3727)
                     /gene="tolB"
                     /locus_tag="ACHY3M_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFS4319119.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3860..5080)
                     /gene="tolA"
                     /locus_tag="ACHY3M_004319"
     CDS             complement(3860..5080)
                     /gene="tolA"
                     /locus_tag="ACHY3M_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFS4319120.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAA
                     AEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIE
                     SKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQA
                     VYEVFKNAPLDFKP"
     gene            complement(5145..5573)
                     /gene="tolR"
                     /locus_tag="ACHY3M_004320"
     CDS             complement(5145..5573)
                     /gene="tolR"
                     /locus_tag="ACHY3M_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFS4319121.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5577..6269)
                     /gene="tolQ"
                     /locus_tag="ACHY3M_004321"
     CDS             complement(5577..6269)
                     /gene="tolQ"
                     /locus_tag="ACHY3M_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFS4319122.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6266..6670)
                     /gene="ybgC"
                     /locus_tag="ACHY3M_004322"
     CDS             complement(6266..6670)
                     /gene="ybgC"
                     /locus_tag="ACHY3M_004322"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS4319123.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6820..7113)
                     /gene="ybgE"
                     /locus_tag="ACHY3M_004323"
     CDS             complement(6820..7113)
                     /gene="ybgE"
                     /locus_tag="ACHY3M_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFS4319124.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7113..7226)
                     /gene="cydX"
                     /locus_tag="ACHY3M_004324"
     CDS             complement(7113..7226)
                     /gene="cydX"
                     /locus_tag="ACHY3M_004324"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFS4319125.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7241..8380)
                     /gene="cydB"
                     /locus_tag="ACHY3M_004325"
     CDS             complement(7241..8380)
                     /gene="cydB"
                     /locus_tag="ACHY3M_004325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS4319126.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8396..9964)
                     /gene="cydA"
                     /locus_tag="ACHY3M_004326"
     CDS             complement(8396..9964)
                     /gene="cydA"
                     /locus_tag="ACHY3M_004326"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFS4319127.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2519 a         2788 c         2460 g         2509 t
ORIGIN      
        1 atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc gaccaattga
       61 ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta cctgaaagat
      121 ttctactcgg ggccaatctt taagattggt gtgtcgtgca ggatgactcg cttcgcttgc
      181 cctacgggcc gttgctggcg caacgttatc cttcacgctt aacatctgag tttgatgtta
      241 aaattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
      301 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
      361 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
      421 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggatga ctcgcttcgc
      481 tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttaacatc tgtcttcgat
      541 gctaaaactg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
      601 ctctaccaac tgagctaacg acccggtggt gggtgatgac gggatcgaac cgccgacccc
      661 ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccaatactac gctggatact
      721 acaactaact atgttagtgg tgggtcgtgc aggatgactc gacttcgtct cgcccttcgg
      781 gccgttgctg atgcaacgtt atccttcacg ttttactacc gcttccacct tagcgattgg
      841 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
      901 gagctaacga cccacttttt cgttgctttc ggtttgtttg atatcccgtg gcaacggcgg
      961 catatattac tgatttcaga cttgagcgca acaaaaattt cgatgcagat cactcaactg
     1021 cttatgattc gcacgacacg accagaaata atgcgacttc tggtcgtgtg ttatgcatta
     1081 catcgcgttc agacgttttt gtgcctgttt agcgccatca gtaccagggt atttactgat
     1141 aacctgctgg tatacggctt tcgcttttgc ggtgtcacct ttgtcctgca tgatgacgcc
     1201 gactttaaac atcgcatcag cagcctttgg tgacttcgga tagtttttca ctaccgaagc
     1261 aaaatagtac gccgcatcat cttttttacc cttgttgtag tttaactgac ccagccaata
     1321 attggcgttt ggcaggtaag ttgaatcagg gtaatttttg atgaaattct gaaatgccac
     1381 cattgcgtca tcctggcggg atttatcctg caccagcgca atagctgcat tgtaatccgt
     1441 gtttgcatcg ccgcttttca ccggcgcgcc agcattcgca gtaccagcat cagctgtcgg
     1501 cgtcgttgat gccgccgcac cgctttgatc gctgctggtt gattgcgccg ctgcaccacc
     1561 gctgctgagg ctgtcgatct gcaacaggat ctgcttttgc cgctccacga cctgattcag
     1621 ttgatactga ttttcctgaa tctgaccacg cagggaatca atatcggatt gattatcaga
     1681 aagttgttgc tggagttggg ttaaaagctg gctgtgagca ttagaaatac gctcaagttg
     1741 agtgacgcgg tcttcgaccg agcctgagcc gacactactg attggtgcct gagcaaaagc
     1801 ggcccagggg gccgctatac caaccagtaa cgacagactc aatagttgat gtctgaagtt
     1861 actgctcatg caattctctt agtaaaccag taccgcacga cggtttttgg agtatgccgc
     1921 ttcgtcatga cccagtactg caggtttttc tttaccgtaa gaaacgatgg agatctggtc
     1981 tgcagaaacg cctttaccct gcaggtacat cttaacggcg ttcgcacgac gttcacccag
     2041 ggagatgttg tattccggag taccacgttc gtccgcgtga ccttctacgg tgactttgta
     2101 agacgggttg ctacgcagga agtttgcatg tgcatccagc atttgagcga agtcagaacg
     2161 gatatcgtac ttgtccagat cgaagtaaac gatgttgttc tgctgcagct gttgcatttg
     2221 cagacgagcc tgctcttcgg aagacatgtt gccgttgccg ccgttcgcat ccataccagt
     2281 gccggcaccc agcatgcctt cgctgccgtc attgctggcg ttcttgttgg aagaacatgc
     2341 cgcaattgcc ataacaggca gagcaatcat cagccctttc agcactttgt tcagttgcat
     2401 ttcaatgatt cctttactat tcaattaatt attatcacag atacggcgac caggcaggga
     2461 atttgacctg tccatcagtt gccggaagac gcgctttgaa acgcccatct gtagaaacca
     2521 aattcagcac ggatcccatc ccctgagaag agctgtagat taccatagtg ccgttaggtg
     2581 ccagacttgg cgtttcatcc aggaacgtgg acgacagaac ttgtacgcct cccgttgcca
     2641 gatcttgttt ggcaatgtgc tgctgcccac cattggagct gaccattacc ataaatttac
     2701 cgtcgctgct gacatccgca tcctggttct gcgaaccttc ccaggtaata cgttgtggcg
     2761 caccgccgtt gatattcact ttataaacct gcggacgacc ggcctggtca gaagtaaatg
     2821 ccaggttctg gctatccggg aaccaggtcg gttcggtatt gttactgcga ccatcagtca
     2881 cctggcggat ctgaccagaa gccaaatcca ttacgtacag gttcagacta ccggttttcg
     2941 acaaggcgaa tgccagtttg ctgccgtctg gcgagaatgc aggtgcaccg ttgtgacgcg
     3001 ggaatgaagc cacctgacgt acagcgccat ttgccagcgt ctgaataacc agcgcggaac
     3061 gaccgctttc gaaggtcaca taagccagtt tagaaccgtc tggtgaccac gccggagaca
     3121 tcagcggctg cggtgaacgg tgaacgacaa actggttgta accgtcatag tcagatacgc
     3181 gcagttcata cgggaactga ccgccgttgg tctgaacaac gtaggcaata cgagtacgga
     3241 acgcaccttt aatgccggtc agtttttcaa acacttcatc actggcggta tgaccagcat
     3301 aacgcagcca ctgcttgttc actttgtacg agttctgagc aagtacagta cccggtgcgc
     3361 cgccagtgtc aacaagttga taagcaacat tgtaagaacc atccggattc ggagtgacct
     3421 gaccgacaac tacagcgtca atgcccagtg cggaccatgc agctggttgt acttcctgcg
     3481 cactacccgg ctgctgtggc agacgagcgc gatctaacgg attaaattta ccgctgttac
     3541 gcaagtcagc agcaacgatg ccgccaatat cttcaggtgc cgcaccaggc cccgcccact
     3601 ggaaaggaac aacaccaata ggacgaccgg aatctacacc gctgtcgatc acaatgcgga
     3661 cttcagcatg cagaactgat gcccacagta tgagaaaacc aaatgctact cgtaatgcct
     3721 gcttcatcat atctccctta tctggaccga tggcccacga taatttagca gaatgttaac
     3781 aaactaaaat acacaaaact accagaaccc cgtggcaacc ggtgcctgat gttgaccgtc
     3841 cgaacagtca acatcgcgat tacggtttga agtccaatgg cgcgtttttg aacacttcat
     3901 atactgcctg gcttggtggt ttcgggatct tcgcaagttt agctgctgcc aacgcagcct
     3961 gacaaagtgc gggatcgcca ccttcaggtt tgatatccag taacatacca tcgggtgcca
     4021 gttttatgcg cagcgtacag gttttgcctg cataggacga tgcgtcatag aacttacttt
     4081 cgatagcaga tttaatctgc ccggcatagt tattgatatc ggcccctgat gcgccattgt
     4141 ttttagtatt accactcccg gcaggcgaag cattgttccc tttcgcccct ccccccgttt
     4201 tcggtgcatt cttaccagag cttagctcac cgaaaatatc atctgcctct gcggccgctt
     4261 ttgctgcagc ggctttttca gctgcagctt ttgccgctgc cgcttttttg tctgcggcgg
     4321 ctttttctgc cgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
     4381 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc tttggctttt tcagcagctt
     4441 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
     4501 tcagtgccgc agcggctgcc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
     4561 ctgcttctgc ttttttcttt gcgtctgcag ccgctttctt cgctgcttct tccgcagctt
     4621 tagcatctgc ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcctcttcag
     4681 cttgcttctg ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgctcct
     4741 gagccgctaa ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct
     4801 cacgcagttc ttcagcagcc tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg
     4861 cgcttgattc ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca
     4921 tgacagcgtc gatggacgaa ccaccgccgc ctccggctga agcttctata ttctcatcga
     4981 acgaactcca gatcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg
     5041 cccgcttgag cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt
     5101 tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc
     5161 attaaaccaa ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca
     5221 tcgtaaggca catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa
     5281 cggctggaca cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca
     5341 accaccacgg tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta
     5401 ctgctcaccg cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg
     5461 atgggcgctg tcgccatgaa gatcagcaac agcaccagca gtacgtccag caacggtaca
     5521 atgttgattt cggacttaag ttcgcgacga cctcgtccac gcgctctggc catgacttac
     5581 cccttattgc tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc
     5641 ataaagttgt cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttgtaggcc
     5701 ataactgccg ggatagcggc aaacagacca attgcagtcg caatcaacgc ttctgcgata
     5761 ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc
     5821 atgatccccc agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg
     5881 aggaacggaa tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca
     5941 cgcgacgccc cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca
     6001 aactctttga acccgctgta aaatatttgt tccgaaccag tcagattatc ccgtttcccc
     6061 tgactctctt gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct
     6121 tcggcttcgc gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaggat
     6181 gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg
     6241 aacaaatcaa ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa
     6301 gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca
     6361 gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg
     6421 atgttatttc agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca
     6481 ccgtcatttt acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt
     6541 gatgatgacg cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt
     6601 acaccacacc accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg
     6661 ttgtattcac tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact
     6721 atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg
     6781 gcctggagcg gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag
     6841 cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt
     6901 ctgcggacga aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa
     6961 accatgccag atttccagtt cactggtctt cgcggcaaaa cgagatgggt cccaaaacat
     7021 acatcctgct aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat
     7081 taccgcatac aaagtcgcga taatcttact catcagatgt cttcttgacc ggctttgcct
     7141 gattcgacgt gttcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt
     7201 cccagaatcc atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt
     7261 tacgttcaat atcttctttg gtgatacgac cgaacatttt ccagtaacac caggcggtgt
     7321 agagcagaat gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct
     7381 ggctggaagt tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca
     7441 caaacgggaa cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga
     7501 acacaaacgc ccacgcggct ttatccatac gtgcagtcag gatggtcagc agcggcagaa
     7561 ccacacccag tgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg
     7621 cgccagcttc acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg
     7681 tcgatttcac gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac
     7741 agaccagtgt caccagcgca gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc
     7801 ccacggtacg catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca
     7861 cgcctgccag caggccgaac gggttaagca actggaagaa gttaccggtg tagtacagac
     7921 gcagatattc atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa
     7981 ttaccagcgg cggaacgaag ctaccaatga agatgcccca gtcccacatg ttacgccagc
     8041 gggtttcttc aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaagacgcca
     8101 gcacgaggat catcgccaca tagaagccgg agaacgcagc ggcatagacc atcggccagg
     8161 cagcaaagag tgcgccgccc gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg
     8221 caatggagtt aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca
     8281 tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca
     8341 gcagccacca gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc
     8401 gtgccggctg agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc
     8461 ttgggccgag gcgtgcaaat ttgaacatta agaacaattc tgccaccagg aacagggtat
     8521 acaggccgca aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg
     8581 ccacagctgt cggcagcact tcaccgatag cccacggttg gcggccatat tcagccacga
     8641 accagcccgc ttctacagca atccacggca gcggaatacc gtacagcgcg gcgcgcagaa
     8701 gccatttttt ctcgccaatg cggttgcgga tgacactcca gaaagagagc gcgatgattg
     8761 ccagaagcag gaagccacac gccaccatga tacggaacgc aaagtacagc ggcgctacac
     8821 gcgggatgga gtctttggtt gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg
     8881 gcgtatagcg tttcagcagc agaccgtaac cgaggtcttt cttcatgcta ttgaactggt
     8941 cacgaaccgc ctggtcggta gaaccagaac gcagttgttc gagcagagag tacgccttca
     9001 tcccgttacg aatgcgttct tcatgctgca ccatcagctc tttcaggccg ataaccgggg
     9061 tatccacgga acgcgttgca atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt
     9121 tcgtctcctc ttcctgatca ggaatgccga acagagtaaa ggcagcaggc gcaggttgcg
     9181 tttcccactc ggcttcaata gcagccagtt tggttttctg cacgtcgccc atttcgtagc
     9241 cggattcatc acccagaaca ataacagaca gaacagcagc cataccgaag ctggcagcga
     9301 tagcaaagga gcgtttagcg aaggcgaagt cacgaccttt cagcatatac catgcgctga
     9361 taccgaggat gaacatcgcg ccagtcacat aaccagacgc tacagtgtga acgaatttca
     9421 cctgagcaac cgggttaagc accagctcgg agaagctcac catctccata cgcatagttt
     9481 caaagttgaa atcggacgcg attgggtttt gcatccagcc gttcgcaacc agaatccaca
     9541 gtgcggacag gtttgaaccg agcgccacca gccaggtgac acacatatgc tgaactttac
     9601 ccagacgatc ccaaccgaag aagaacagac ctacaaaggt ggattcgagg aagaaggcca
     9661 tcagaccttc gattgccagc ggcgcaccga agatatcccc tacatagtgg gaatagtaag
     9721 accagttagt cccgaactgg aactccatgg tcagaccggt agccacaccc agagcgaagt
     9781 tgataccaaa caacttgccc cagaacttgg tcatatcttt ataaatctgt ttgccggaga
     9841 ggacgtagac cgtttccata atggccagca ggaacgccat accgagcgtc agtggcacaa
     9901 aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg actatatcta
     9961 acatcatgac tccttgctca tcgcatgaag actccgagag tgaaccccgt tagaaagggt
    10021 cacacgcatg ccccaataga aatttgtttg aacactttca tcataccatg tgaatgcaca
    10081 caaaaacggc gtaaagaaag gttgcaaaca tgttaataaa aactcaaatt gatcccacgt
    10141 atatattacg ccgcaaaatc cttacaataa acaggttttt gttgaagcaa atttgcattt
    10201 ttcgacggtg atcaatttat agctaaatta ccgcctttca gccaatttga tcgagaacaa
    10261 tttatctctt tttgat
//
LOCUS       DAZUFB010000138        10258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZUFB010000138 DAZUFB010000000
VERSION     DAZUFB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.138"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1195
                     /gene="ybaT"
                     /locus_tag="ACHY3M_004327"
     CDS             <1..1195
                     /gene="ybaT"
                     /locus_tag="ACHY3M_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4319128.1"
                     /translation="LLGQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGI
                     IDFFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYA
                     LGIIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSG
                     AFFSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSD
                     VSALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGS
                     ERELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVA
                     IRLRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMK
                     RNKTV"
     gene            1226..1323
                     /locus_tag="ACHY3M_004328"
                     /pseudo
     CDS             1226..1323
                     /locus_tag="ACHY3M_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1320..1727
                     /gene="cueR"
                     /locus_tag="ACHY3M_004329"
     CDS             1320..1727
                     /gene="cueR"
                     /locus_tag="ACHY3M_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFS4319129.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1728..2186)
                     /gene="ybbJ"
                     /locus_tag="ACHY3M_004330"
     CDS             complement(1728..2186)
                     /gene="ybbJ"
                     /locus_tag="ACHY3M_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4319130.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2183..3100)
                     /gene="qmcA"
                     /locus_tag="ACHY3M_004331"
     CDS             complement(2183..3100)
                     /gene="qmcA"
                     /locus_tag="ACHY3M_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4319131.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3246..3923
                     /gene="fetA"
                     /locus_tag="ACHY3M_004332"
     CDS             3246..3923
                     /gene="fetA"
                     /locus_tag="ACHY3M_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFS4319132.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3910..4692
                     /gene="fetB"
                     /locus_tag="ACHY3M_004333"
     CDS             3910..4692
                     /gene="fetB"
                     /locus_tag="ACHY3M_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFS4319133.1"
                     /translation="MNSHNITNESLALALMLVVVVAILISHKEKLALEKDIFWSVGRA
                     IIQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITV
                     GAGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEK
                     LSLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKY
                     QIMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4755..5609)
                     /gene="cnoX"
                     /locus_tag="ACHY3M_004334"
     CDS             complement(4755..5609)
                     /gene="cnoX"
                     /locus_tag="ACHY3M_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFS4319134.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5670..6479)
                     /gene="ybbO"
                     /locus_tag="ACHY3M_004335"
     CDS             complement(5670..6479)
                     /gene="ybbO"
                     /locus_tag="ACHY3M_004335"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS4319135.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6469..7092)
                     /gene="tesA"
                     /locus_tag="ACHY3M_004336"
     CDS             complement(6469..7092)
                     /gene="tesA"
                     /locus_tag="ACHY3M_004336"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFS4319136.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7063..7749
                     /gene="ybbA"
                     /locus_tag="ACHY3M_004337"
     CDS             7063..7749
                     /gene="ybbA"
                     /locus_tag="ACHY3M_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFS4319137.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7746..10160
                     /gene="ybbP"
                     /locus_tag="ACHY3M_004338"
     CDS             7746..10160
                     /gene="ybbP"
                     /locus_tag="ACHY3M_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFS4319138.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGQAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2247 a         2640 c         2868 g         2503 t
ORIGIN      
        1 gctgctgggg caggctgcat tgctaatgga agcctcgacc tgggtcgcct ttgcttttgg
       61 cggtattgtg gcgatgtttt ccggttatgc ctatgcgcgt ctgggggcga gctatcccag
      121 caatggcggc attatcgact tctttcgtcg cggattaggc aacggcgtct tttcgctggc
      181 gctctcgtta ctgtacctgt tgacgctggc ggtgagcatc gccatggtcg cccgtgcttt
      241 tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc ttattttgct
      301 ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca accatgcggt
      361 agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat tgctgattat
      421 tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc ccagctccgg
      481 tgcgttcttc tcctgtatcg ggataacctt ccttgcctat gcgggctttg gcatgatggc
      541 gaacgcggcg gataaagtga aagatccgca ggtcattatg ccgcgggcgt ttctggtggc
      601 gattggcgtt accacgttgc tttatatctc gctggcactg gttttgctta gcgatgtatc
      661 ggcattagag ttagaaaaat atgccgatac cgccgtagcg caggctgctt ctccgctgct
      721 cggacatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg cttcagccat
      781 taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg aacgcgaact
      841 gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaaca tcattgtcgt
      901 ggtgttgatt atgctgatga cggcggcact aaatttaggc tcactcgcca gcgttgccag
      961 cgccaccttt ttgatttgct atctggcggt gtttgtggtg gcgatccgcc tgcgtcatga
     1021 tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt tggtgatcgt
     1081 tggctttatc tacagtctgt ggtcccaggg tagccgtgcg ttgatatgga ttattggctc
     1141 actcttactc agccttattg tggcaatggt catgaagcgc aataaaaccg tataacatct
     1201 ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca ctggcaaaaa
     1261 aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt gcgcgaacga
     1321 tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt cgcttctatg
     1381 aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc acctacacgc
     1441 agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc tttaacctgg
     1501 aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc gccgacgtca
     1561 aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag ctgcaatcca
     1621 tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc gccgactgcc
     1681 cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta agacgagacg
     1741 gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt acctgcaccg
     1801 agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg
     1861 ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt
     1921 aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc
     1981 caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca acccagcggc
     2041 accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa caaataacca
     2101 tttccgccca gcatctcggc tgccagcagc aaaccgccga gactgagcca gaaaatatgt
     2161 ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca
     2221 gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta
     2281 ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttc tgcgctacga
     2341 agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt ttggtggcgc
     2401 gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt
     2461 cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct
     2521 gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg
     2581 cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga
     2641 ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt gagttgatgc
     2701 tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag
     2761 tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgcggcg
     2821 cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga
     2881 taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc
     2941 gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa
     3001 aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga
     3061 ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta
     3121 ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga
     3181 gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa
     3241 cagtgatgca ggaaaatagt cctttacttc agctacaaaa cgtaggatat ctggcgggtg
     3301 atgcgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta
     3361 ccggtccttc cggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc
     3421 caaccaacgg aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct
     3481 accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg
     3541 ataatctgat ctttccctgg cagatccgta accagcagcc tgacccggcc atttttctcg
     3601 attttctcga acgcttcgct ttgccggaca gcattttgac gaaaaatatc gccgagctat
     3661 ctggtggtga aaaacaacgc atctcactga ttcgtaacct gcaatttatg ccgaaggttt
     3721 tattgctgga tgaaatcacc agtgcgctgg atgaaagtaa taaacataac gttaatgaga
     3781 tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag
     3841 acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg
     3901 aagcacgcta tgaactcgca taatattact aacgaatcat tggcactggc attaatgctg
     3961 gtggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
     4021 ttctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt gctgaagtat
     4081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
     4141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
     4201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc tgggtcgatt
     4261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
     4321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
     4381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
     4441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
     4501 ttacctggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
     4561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
     4621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
     4681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
     4741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
     4801 gcatcacccg tacccagcgc agcgaggatc tcctgaaacg ttttacgcgt ctgaccgtct
     4861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
     4921 ccaacctggt gcagttgcag agccagttgc gtcgccagtg cggcatcttc tggattctcc
     4981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
     5041 atttgcgcca ccagcccctg gtagcgggtg tcctgatcct gcaacggaat ggttttcagc
     5101 accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag cagcaggccg
     5161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
     5221 taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
     5281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
     5341 accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc aaactgggcg
     5401 gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
     5461 gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg ttcagaccaa
     5521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
     5581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
     5641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
     5701 cccggcagca ggcgcttgag caccattact gcccaggtca ccagcgttac cggatagcgc
     5761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
     5821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
     5881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
     5941 ctgtggcgca gctccatgcg cagtgcgtct gaccacgcct ccagcgcata tttactggcc
     6001 gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt catcacaata
     6061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
     6121 aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga aagggggcca
     6181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
     6241 acctcgtctg ctgcgcgatc aacactttct ggtgaatcca gatcgatcaa cacgccggta
     6301 aatcccatgt tgttcatacg ctcaacatca tccggttttc ggcaacctgc cagcacatga
     6361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
     6421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
     6481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
     6541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
     6601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
     6661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
     6721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
     6781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccagcgcg gctgatgctg
     6841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
     6901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
     6961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
     7021 ggcggcacgg aaggttaaca ggaccaggaa caggaagggc aaatgccagc ggaaaacatt
     7081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
     7141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
     7201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
     7261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
     7321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
     7381 gaaaatgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
     7441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
     7501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
     7561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
     7621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gactcacgac
     7681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
     7741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgctc gccgtcgcta ttaattgtct
     7801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
     7861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
     7921 gttcgcgcga agtgccgcaa gcatggctgg aggaagcgca aaagcgcggc ctgaaagtcg
     7981 gcaagcagct gactttcgcc accatgacat ttgcaggcga cacaccgcag ctggcgaacg
     8041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
     8101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
     8161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
     8221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
     8281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
     8341 tcggcggcaa cgagaaccag ctcgacggct atgagaaatg gttgttacct cagcttaaac
     8401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
     8461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
     8521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
     8581 cgctgggggc agggcaagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
     8641 tggcgctttc agccgttacc ggtggggcga taggcctgtt gttcgaaaac gtgttgatgg
     8701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
     8761 gggcgctggg taccatgacg gtcatctcgc tgctggtggg gctacgaccg taccgcctgt
     8821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
     8881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
     8941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
     9001 gtgtgctggg ctggatgctg ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
     9061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacattaagc cagctttcgg
     9121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgcttg
     9181 accgctggca gcagcagcta cctccagaaa gcccgaacta ctttttaatt aacattgcca
     9241 cagaacaggt agaaccgcta aaagcgttcc tcgcggaaca tcagatagtc ccggaatcat
     9301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
     9361 aagatgaggc gcttaaccgc gaactcaatc ttacctggca aaatacgcga cccgatcata
     9421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
     9481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
     9541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
     9601 tctattttat tttccctgaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
     9661 tccgctggga gaatggcaac ggcatgttga cacaactcaa ccgccagttc ccgaccatta
     9721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
     9781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
     9841 aggtgcaggt gggaatgcgc cagcgtcatc aggagctggt ggtatggcgc acactcggtg
     9901 cggggaaaaa actgctgcgc acgacattgt ggtgtgagtt cgccatgctt gggtttgttt
     9961 ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc agtgttgcag gcgaaagtgt
    10021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcgc
    10081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    10141 tcaggcagtt tgcggggtga tgagagtaaa taaggggcgt tctggttgaa tggaacgcct
    10201 tgtttaaatt aattctacaa tatgtttcat tgatgatgtt attgaattgg tgatttcc
//
LOCUS       DAZUFB010000139        10003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZUFB010000139 DAZUFB010000000
VERSION     DAZUFB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.139"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(369..444)
                     /locus_tag="ACHY3M_004339"
     tRNA            complement(369..444)
                     /locus_tag="ACHY3M_004339"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Asn,seq:gtt)
     gene            complement(545..2033)
                     /gene="yeeO"
                     /locus_tag="ACHY3M_004340"
                     /pseudo
     CDS             complement(545..2033)
                     /gene="yeeO"
                     /locus_tag="ACHY3M_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2194..2269
                     /locus_tag="ACHY3M_004341"
     tRNA            2194..2269
                     /locus_tag="ACHY3M_004341"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2227..2229,aa:Asn,seq:gtt)
     gene            complement(2307..3257)
                     /gene="cbl"
                     /locus_tag="ACHY3M_004342"
     CDS             complement(2307..3257)
                     /gene="cbl"
                     /locus_tag="ACHY3M_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFS4319139.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3359..4276)
                     /gene="nac"
                     /locus_tag="ACHY3M_004343"
     CDS             complement(3359..4276)
                     /gene="nac"
                     /locus_tag="ACHY3M_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFS4319140.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4603..4678
                     /locus_tag="ACHY3M_004344"
     tRNA            4603..4678
                     /locus_tag="ACHY3M_004344"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4636..4638,aa:Asn,seq:gtt)
     gene            complement(4734..5669)
                     /gene="ldtA"
                     /locus_tag="ACHY3M_004345"
     CDS             complement(4734..5669)
                     /gene="ldtA"
                     /locus_tag="ACHY3M_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4319141.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVKRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5731..6810)
                     /gene="cobT"
                     /locus_tag="ACHY3M_004346"
     CDS             complement(5731..6810)
                     /gene="cobT"
                     /locus_tag="ACHY3M_004346"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFS4319142.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKLGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6822..7565)
                     /gene="cobS"
                     /locus_tag="ACHY3M_004347"
     CDS             complement(6822..7565)
                     /gene="cobS"
                     /locus_tag="ACHY3M_004347"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFS4319143.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7562..8107)
                     /gene="cobU"
                     /locus_tag="ACHY3M_004348"
     CDS             complement(7562..8107)
                     /gene="cobU"
                     /locus_tag="ACHY3M_004348"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFS4319144.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8259..8432)
                     /locus_tag="ACHY3M_004349"
                     /pseudo
     CDS             complement(8259..8432)
                     /locus_tag="ACHY3M_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(8411..8599)
                     /locus_tag="ACHY3M_004350"
                     /pseudo
     CDS             complement(8411..8599)
                     /locus_tag="ACHY3M_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            9779..9994
                     /locus_tag="ACHY3M_004351"
                     /pseudo
     CDS             9779..9994
                     /locus_tag="ACHY3M_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT         2820 a         2595 c         2098 g         2490 t
ORIGIN      
        1 atattgatta taaaaaatcc ccgttcccag agaaataata tttattaaaa ttccagttct
       61 tctttttctg attacagaag gcaaagtggc aataacgcat agtttcccga taaagacgcg
      121 atagcgacat cccgcataag gcatttttct ctttatcttt gtacggtact tcatggaaca
      181 gagtttttga ccttgcgaat cgtgatgtct gttgggggca acttgctcac tgaagcgtga
      241 gactcgatta agcgcacgaa acacagaaat caaaaaaccc ggtcactttt ttacaaggta
      301 accgggtaaa aataattttt attttttaac tgttttgaga ctcatagaga tgtctcaaaa
      361 ctaaaatttg gctcctctga ctggactcga accagtgaca tacggattaa cagtccgccg
      421 ttctaccgac tgaactacag aggaatcgtg agaacgaggc gaatattagc gatgcccacc
      481 cataatgtca aagcctgttt tttaaatttg aaatcgtttg ctgaaataat ctgcattttg
      541 tcgtttattc cgacacagct ggcttttttt cacacttttg cggttcggat cgagggtatt
      601 tccacagcca acgtccagta accattcgcc agtaaaacag cacggcccgc acagcccagt
      661 cggcaaacat tcccatccag acaccaacca caccccagcc aagcatgatg cccagcacat
      721 aaccgacgac aacccggcaa ccccacatgc tcaacatcga aacccacatg gcgtaacggg
      781 catcacgagc acctttaaat ccagcgggta gcacccatga ggcggaccaa ataggcataa
      841 ataaagcatt tagccaaatc agaatcacaa cgacatgttt aacctgtgga tcctgggtgt
      901 aaaacgatgc cataagcccg gcaaagggag ccgttagcca ggcgatggcc gttaatccaa
      961 gagtggacag ccagaacaca tgccgcaact gaatctctgc ttgcgctatc tgccctaccc
     1021 ccaaccttcg gcctgtaatg atcgtagaag cagagccgag cgcacttccg ggtaagttga
     1081 taagagccgc aattgaaaac gcgataaaat ttccggcaat aacactggtc cccatcccgg
     1141 caacgaacat ttgggttaat aaccgaccac tggtaaataa cactgattcg acactcgctg
     1201 gaataccaat ccaccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc
     1261 tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact
     1321 gcgccaatat aacgagaaat gattaaaccc aacccagccc cgacaaatcc cagtcccggc
     1381 caggagaaaa ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta
     1441 ccgttaatca gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaatc
     1501 aaagtgatgg cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt
     1561 gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga
     1621 tgaataagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc
     1681 gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta
     1741 ccaagatcga tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc
     1801 acgccggcca tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacacccatc
     1861 aacaggacac aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag
     1921 aacaacactt tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt
     1981 aaagcagagg tgatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat
     2041 aatgagggag aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa
     2101 caaactgttg atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga
     2161 tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg
     2221 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa
     2281 agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt
     2341 tccatcacct gccgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag
     2401 cgccagacat agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca
     2461 tcgaaaagat gccgcgtatc cagacggatt aaattctctt cctcttgttc gccgctggat
     2521 tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa
     2581 tcctgcgcac ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata
     2641 cgtgagcgcc ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc
     2701 agcgtcaatg gtgaaatttg cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc
     2761 caacgaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata
     2821 ccaatatcag cttcgccatt ttgcaacaat gtcgcaattt cctgtggcgt cccctggatt
     2881 agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta
     2941 taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac
     3001 aggtctgcaa gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt
     3061 aatgctttgc ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg
     3121 atgccaagtt catcctccag ttcccgaata tgacggctga cgcctgactg tgaggtaaaa
     3181 agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt
     3241 agttgttgga aattcacggt aaactccggg cagttcagat ttcccgttat tgttaaagtc
     3301 taatgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga tataacactt
     3361 agctcaccaa ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca
     3421 actctttcac tgcctgtgcc tgtggcgata agttcgctct ggcgggtaaa tttaatgaca
     3481 aagagagact catggaagga gtggtaatgc gtgacatcca cccatttact gcgccacata
     3541 acgaacgcgc ggccgattcg ggtaatactg caacgcccat gccgctggca atcgctgcgg
     3601 taagcgtggc aatagactca atttcaccaa taacttttgc cgtgagtcgc cgtagggaaa
     3661 aagcctcatc aacacgaagt ctaatagcac tgtaatcact ggggagaaag aggttcattt
     3721 tcgcaatagc attcacatca acgctttgcc ccgggcaatc ttgagttcct accagaaaaa
     3781 gatcttcttt cagcaaagcc tgactggata caccagccac aggggaatgc tcataaatca
     3841 ccgccatatc gagttggtga tttatcaatt tttcgttaag cactgcacca ctattttcat
     3901 gaagatagat aacgatctcc ggaaattcag cgcgaaccgc ctgtaataag ggcatggtga
     3961 tggatgacgc agcggttcct ggtgcaaagc caatcgagac ttgccccgat aatgcctgac
     4021 caacgttatg caccgccagt tgggcctgtt cacactgacg taaaatggcc cgcgcatggg
     4081 tatagagaat ttttccggcg tctgttggcg taacgccccg ctttgtacgg atcaaaagtt
     4141 gttgatttaa ctcaccttcc agtgtggcaa cctgctggct gagcgctggt tgtgcgatat
     4201 gcaatacttc agcagcctgg gtcaggctac caatatctac aatttttacg aagtatttca
     4261 ggcgtctgaa gttcatgttg cctccggttt ttaagaatcg gcccaagtgc cgccattact
     4321 tacaaccaga ttgcaagatg cttgccagtt ttattttggc gttgatgtac aagctaacca
     4381 actgtcaaat aagagattat gatagattcg tcatttgctc ctttaatcag ctgtcgcgtt
     4441 cccctgccct ataaaaggag ggtatgcacc acgatgattc attacccaat aagattgaaa
     4501 gctcaccact ttgttgaaat tgacagcaaa caaacaaaaa aatgcatttc accctttgac
     4561 atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt agttcagtcg
     4621 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     4681 ttcaaaaaag cctgctttct agcaggcttt ttgctttcta attaccaacg ctcttaaaac
     4741 atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgacgttg caacgcagca
     4801 tttgcgcgtt ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga tggggttacc
     4861 ggcaatggta cttttcggtc agactcatat tctgcacgat tgcgcgacag cggctcatga
     4921 acttccagcc agttcgagcc atctggttca gtggtgtatt ttactggctg gtcgataatt
     4981 tgcacacgcg ttccaacagg aacattatca aacagatatt tgatatcgtc attgcgcaga
     5041 cgaatacagc cctgacttac ccggagccca ataccaaaat tggcattggt accatggatg
     5101 gcatacaacc tgccaatata aatcgcgtac agccccatgg gattatcggg acccgcagga
     5161 acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc
     5221 gtccaggttg gagcttcttg tttacgttca acggtagtca cccagttacg cggggtttct
     5281 cgcccagcct ggccgatacc aataggaaag acttccacag tattactgtc tggcgggtag
     5341 taataaagac gcatctcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa
     5401 atcagttgct gcggaatggt aagttgcgag ccagacttcg gcaagaaaac atcagcaccc
     5461 gggttcgctt ccagcatgtt acttaaccct tgcccgtttt gtgcggcaaa agtctccagc
     5521 ggctgggtat tgtgatcagg aacagttaca gtatacgact gccccactaa acggctaccc
     5581 tctggaggta atggataagt taccgccagg ctagtatggc tggcaaaaag cagagcaaat
     5641 gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa agctatccgt
     5701 taagtatagc ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc
     5761 cggtagaaca atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc
     5821 ttcgatgatg ggcatcgcca gagctgcgcc actcccctca cctaaacgca tctccatatt
     5881 gagataaggc tccagcccca aatgcgacag cgcaatacgc gccccttttt ctgccgacag
     5941 gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcggc
     6001 agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat
     6061 cacgccagcc attccgacca aatcaaatcc acccaatttt gccaggacat caacgccatc
     6121 ctgaggattt ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag
     6181 tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc ggccagtgat
     6241 tgtgctgact attgctgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa
     6301 cagcgtaaca ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt
     6361 ttcagcctga tggcgactca ttgccggagc tgaagcaata ttgccactac ctcgagcaac
     6421 acgcatgttg ataagcccgg ggataggctc agcagtatca ataccaacat caattacgtg
     6481 gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt
     6541 ttcagcctga atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc
     6601 gtgatcggca cacatgacca gtaccgcttt tttgcccaca tgcggaacgc cattcaaccc
     6661 cggcattcct gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt
     6721 gagtaacccg tcgatatgcc gttgtgcacg cgacatagcg gcaggatcga tggcagggat
     6781 cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg
     6841 aagaccagtt caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc
     6901 gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc
     6961 acaccatgca taccaggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg
     7021 caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc ttcccgggca
     7081 taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc
     7141 agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc
     7201 agtacaacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt
     7261 tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg
     7321 tccaggtgga atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt
     7381 ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat
     7441 aatccaatta aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg
     7501 gaccagcgac gcggtacagg cagacgcgta ataaaagaga gcatcgccca aaataattta
     7561 ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt gccgcagcgg
     7621 ccaactgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg
     7681 gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg caacgttggc
     7741 aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc
     7801 attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa
     7861 cgcattcaag caacacaacc tcattcgggt taatgtctgc atgaattaat tcatcaagat
     7921 gttgccagcg ctccactgtg cgccagtgct ccgggcggcc ttgccgatga tgttctatcc
     7981 gtgcagccat ctcatcatca aggatttgcg aggtagcgat atacagaacc tgtgaagagt
     8041 ccccaataag cgcctctgcg tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa
     8101 tcatcatacc gactcctgat tcaaatgtat gatggtattc attgaacaga acaatggatc
     8161 ccccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa
     8221 aatccagcat tctcaatcca ttacgaaata agatggcatt acggaatagc cagactttct
     8281 cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctgtggattg acacatttca
     8341 ttcagataat gaattaatgc tactgccgga acaaccagca aacgggcatt gtgttctgaa
     8401 atccaggcta ttgattcaac gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca
     8461 acgtaataga attatccgga atggtggcga ctatgcacta gaacaccaca atttcgctct
     8521 ctcggtaagg cactgtcaca gcattcaaca gaatgtgact tgccagattt attagcgcca
     8581 ccagatgttt aaccgggtaa ccacgaccca gtcggggata tattccatct tctcgctgac
     8641 atcatcaaga ataaaaaggt ttatcacact cagagcaacg ttcttcagca ggcgggataa
     8701 ttcttcaaca tttagaagat gtgttattaa aggcatacaa actggacgta ttgtattttc
     8761 ttttggtggt tgacctaagg tagcagttta tcctgatgcg ctgagatttc tgtaatatct
     8821 acgtcaacat tctcctcgaa tagaaaatgc agccccggca agtaattcac attttttaac
     8881 aaaacgttgt tagcgtgcca ttttcagaat ctctccaaga aagcggattc ttttctcaaa
     8941 ttctgcattc tcagacacag ctttctactc catggtaatg gcccaaatac gaagttgctc
     9001 aggatcatta gtggtgctga gaagttaaat aaccatgcgg agtaccactc gtcatcacgt
     9061 atttatatat catgaagcta ttgttgtgtt ttgtacatca gtagatatat tctgatatac
     9121 tccttttgct agacataacc tttcacctgc ttgcaaagct tctgtgttct gacattgcca
     9181 aattgttgca attctgtatc cagccttctt tcagtcatag cttcgggccg cgataagact
     9241 cactgatctg atcctgattc ctcttgcaga ctttatagac caattaaaat gcagtttctg
     9301 caggtcaacg tctgaccatc attgtcatca ctctggccat tagagtaacc ttctgcattc
     9361 atccttttgt aaaaagttta tattagtatc agcaattaac cggacctgat actgatatga
     9421 gtcttaccgc atatacggtc aatttcagca attaattaca ttatccacgc caaagtattt
     9481 gtcatcacaa tgatggtacc ttctttcaga caccattttt tcaactccgt tttccacgga
     9541 ccgcactctt atgtcaagag tgcggtccgt ggatacaacc agagaccgac tgacacgagt
     9601 cagaggaaac gacggatatg ttcagtcgta aaatatctat caaaaaacat gattaaggtc
     9661 aaaaatgttt gatatttaca atttatgaag atgacaataa ttatagatat atgagaacat
     9721 aaatgaaaat aattatcatt acagcaatta tttgtacttt gtattaatga gggatgaaat
     9781 gttatataat ataccttgtc gaatttatat cctttccact ctgtcattat gcatttctgg
     9841 gatagtttct actgcaaccg caacttcttc agaaacaaaa atcagcaacg aagagacgct
     9901 cgtcgtgacc acgaatcgtt cggcaagcaa cctttgggaa agcccggcga ctatacaggt
     9961 tattgaccaa caaacattgc agaactccac caattgaacc gcc
//
LOCUS       DAZUFB010000140         9721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZUFB010000140 DAZUFB010000000
VERSION     DAZUFB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.140"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..533
                     /locus_tag="ACHY3M_004352"
     CDS             144..533
                     /locus_tag="ACHY3M_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFS4319145.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            530..1456
                     /locus_tag="ACHY3M_004353"
     CDS             530..1456
                     /locus_tag="ACHY3M_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS4319146.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            1456..1677
                     /locus_tag="ACHY3M_004354"
     CDS             1456..1677
                     /locus_tag="ACHY3M_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319147.1"
                     /translation="MRTTKPGKIPGFFVSRSRANSMTIAERLIQKGALEVAREIACRL
                     WNMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(1708..1959)
                     /locus_tag="ACHY3M_004355"
     CDS             complement(1708..1959)
                     /locus_tag="ACHY3M_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4319148.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(1993..2190)
                     /locus_tag="ACHY3M_004356"
                     /pseudo
     CDS             complement(1993..2190)
                     /locus_tag="ACHY3M_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2558..3085
                     /locus_tag="ACHY3M_004357"
     CDS             2558..3085
                     /locus_tag="ACHY3M_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4319149.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            3143..3376
                     /locus_tag="ACHY3M_004358"
     CDS             3143..3376
                     /locus_tag="ACHY3M_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFS4319150.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            3435..5393
                     /locus_tag="ACHY3M_004359"
     CDS             3435..5393
                     /locus_tag="ACHY3M_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4319151.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            5448..5882
                     /gene="psiB"
                     /locus_tag="ACHY3M_004360"
     CDS             5448..5882
                     /gene="psiB"
                     /locus_tag="ACHY3M_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFS4319152.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            5879..6598
                     /locus_tag="ACHY3M_004361"
     CDS             5879..6598
                     /locus_tag="ACHY3M_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFS4319153.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPS
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            6595..7191
                     /locus_tag="ACHY3M_004362"
     CDS             6595..7191
                     /locus_tag="ACHY3M_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319154.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            7654..8154
                     /locus_tag="ACHY3M_004363"
     CDS             7654..8154
                     /locus_tag="ACHY3M_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4319155.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            8885..9319
                     /locus_tag="ACHY3M_004364"
     CDS             8885..9319
                     /locus_tag="ACHY3M_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFS4319156.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            9412..9678
                     /locus_tag="ACHY3M_004365"
     CDS             9412..9678
                     /locus_tag="ACHY3M_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319157.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
BASE COUNT         2108 a         2603 c         2907 g         2103 t
ORIGIN      
        1 ctttaccagg aggaaccgtg agcaagatga aaaacatcat gacgtctatg tcgcatggta
       61 ccggattcct cctgttccat aaaaaaacag agaagggaaa caggttttct ttcattcagg
      121 gcccggacgg gcaggagata aaaatgaagt cttgttttga cactgacaaa gattttcaga
      181 tgtcatctga tgaagtcact ttcaatgctg acagcgttcg ctcaaaactg gaactattat
      241 ccgaagaact aatgcaccgt tgtggtcttg tttatgagct atatcagcga cgtttgagtg
      301 ctgctattga taattttgtg tctacattgc cgacagaaca acatctgctg gtcctggaac
      361 tggcccgtga ggagtttgat tacctttccg ctgaagagat tgcagaagaa atacgccgtg
      421 acgcagaacg tggctattgt tgtcacggct ttgatcgcaa ttgctgtcct cttggttgtg
      481 gggatttgga cgatgattgc tgcgatgctg ctgatttgat ggaggaaccg tgagtaagaa
      541 gaaaaacacc acaacgccca cgccgcatga tgccgcgttc cggtcgttcc tggcgaaccc
      601 cgacgtcgcc agagattttc tggaactgca tcttccggcg gagtaccggc agttgtgcga
      661 cctgtccacg ctgaagctgg aacccgccac ctttgttgag ccggacctgc atcagtacgc
      721 cagcgatatc ctctggagtg tgaaaaccac cgggggtgaa gacggctatg tttatacgct
      781 catcgagcac cagagcaccg aaaatctgta catgcctttc cgcatgttac gttacagtgt
      841 ggcggcgatg cagagacatc tggagcagta caaaacattg ccactggtga ttccggtgct
      901 gttctatcat ggtgagcgca gcccgtaccc gtacagcatg aactggctgg actgttttga
      961 gaatccggcg cttgcggcta aaatatacac aaagccgttt ccgctggttg atatcactgt
     1021 cgttgatgac aatgaaatca tgaaccatcg ccggatggcc gcactgacgc tgctgatgaa
     1081 gcatatccgc catcgtgaca tgatggagct gctggacaaa ctcccgcagg tcatggtgga
     1141 aatttcagac gagcaggtgc gtgttctgat tcattacatc gttaacgcag gggactctgt
     1201 atcaccggaa tttatgcggg cgctggctga gcgtctgccg cagcatgagg ataaactgat
     1261 gactatcgca gaacgtcttg agcaaaaagg tcgccaggaa ggcaggatgg aaggacgcat
     1321 ggaaggacgc atggaaggag cgcttgaaaa agccctggct attgcgtgcc agcttcagaa
     1381 aatggggatg acgccggagc agattaagca ggctaccgga ctttccgatg acgaactgaa
     1441 gaaaatcact cactgatgag aaccacaaag cccggcaaaa tcccgggctt ttttgtcagc
     1501 aggagcagag caaacagcat gacgattgca gaacgtctta ttcaaaaagg cgcacttgaa
     1561 gtggcgcggg aaatagcctg ccggctttgg aatatgggct ggacgccgga acggattcag
     1621 gaggcaaccg gactttccgg tgaagaactg aaaaaactgt ttcctgatga gcagtagcct
     1681 gacattcagc caggctgtga atcacgctca cacatcctct gccgtttccc ccagttcatt
     1741 ttccagacgc ctgatgatat ctgccttacg catcccctgc atgtgaggca gcaggtcacg
     1801 gactgaaaga gcctgggtgg tcctccagac ggtgacgtcc acctgttccg cattttccca
     1861 ggcgtgccat gttctctgcg tcacgccaaa ttctctggcc gctttttcgc gtgaccagag
     1921 catgcttttt cgccagattc gcagttccca gccggtcata aaataccttt gaaaaagtga
     1981 aaaaacttca ttttatttta tcaccataat caccaaatca gcgcgataaa taagtgtgaa
     2041 aaagtgaaga aatttcactt tttttagtta tagatgtcgt gcggttttct ctgttttcac
     2101 ctgtgcggga gtggtgcgcc agtgaccacg acatgccatt gttgtgccgt cgtttttctt
     2161 cagtcgcgcc gtgacctgcg ctccctgcac acctgacggt gtgtgaggaa actgcgcctg
     2221 atggcgcagc agtcttccct cctgccgcca cccctgcggc ctgtttcccg ctgtggactt
     2281 tccggggggc agacgtaacc ccctcccgtt ccatctgtcc agggtgagat gcaccggcca
     2341 caaaattttt agccatgaac ccggctaaaa attttgcgtc cgcccccgga cagctgtccc
     2401 gtgaggcggt aaacgggccc ccatcggaaa ggtccactgc ggggagaaac ccctgtgaag
     2461 tggacggcag gaggagcttt cgctcctctg aaggggcaca tgaacgtccc gtcgccggta
     2521 tccggtttat ctcacatctt tcagtcagga ggttcttatg agtgcacgtg gtatcaacaa
     2581 ggtcatcctc gtcgggcgtc tgggcaatga tccggaagtc cgttacatcc ccaacggggg
     2641 cgcagtggca aacctgcagg tggccacatc agaaagctgg cgtgacaaac agacggggga
     2701 gatgcgggag cagacggaat ggcaccgcgt ggtgctgttc ggcaagctcg cggaagtggc
     2761 aggtgaatat ctgcgcaagg gtgcgcaggt ctacatcgaa ggtcagcttc gcacccgtag
     2821 ctgggaagat aacggtatca cccgttacgt cactgaaatt cttgttaaga ccacgggcac
     2881 cgtgcagatg ctgggacgtg caccacagca gaacgctcag gcgcaaccga agcctcagca
     2941 gaatgggcag ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa cgaaaggccg
     3001 tggacgcaag gccgcgcagc cagagcctca gccgcaaccg ccggaggggg aggattacgg
     3061 gttttcagac gacatcccgt tctgatcggg ctgactgtga caaccgcccc gccctgtgcg
     3121 gggcatcacc ggagatatga ggatgagcga atatttcaga atacttcagg gactgccgga
     3181 cggctccttt acccgcgaac aggcggaagc cgttgccgca cagtaccgga acgtctttat
     3241 cgaggatgat cagggaacgc attttcgcct ggttgtccgt caggatggca cgttgatctg
     3301 gcgctcctgg aattttgagg acggtgccgg gtactggatg aaccagtaca tcagggattt
     3361 cgggattctt aagtaagaga ggtgccggac gcgcgaacgt ccggcaggac ataagcaatt
     3421 ataaggggat gattatgcct gtaacgaagt gtgaaccaga aaccacccgc aaagcaagcc
     3481 gtaaatctgt aaaaacgcag gaaactgtcc tgtctgccct gctggcgcag acggaggaag
     3541 tgagcgtgcc gctggcctcg ctgattaaat caccgctgaa tgtgcgcacg gtgccgtatt
     3601 ctgcggagtc cgtcagcgaa ctggcggagt ccattaaggg cgtcggactg ctgcagaatc
     3661 tggtcgtgca taccctgcct ggtgaccgtt acggtgtcgc cgcaggtggt cgccgactgg
     3721 ctgcactcaa catgctggca gagcgtggca tcattccggc tgactggcct gtacgcgtga
     3781 aggttattcc gcaggagctg gcgactgccg catcaatgac tgaaaacggt catcgtcggg
     3841 atatgcaccc tgccgaacag attgccggat tccgtgcgat ggcgcaggaa ggcaaaacgc
     3901 ctgcacaaat cggtgatttg ctgggctatt cgccccgcca cgttcagcga atgctgaaac
     3961 tggcagacct tgcgcctgtc atcctcgatg cgctggcaga agaccgcatc accacagagc
     4021 actgtcaggc gctggcgctg gagaacgaca ccgcgcgtca ggtgcaggtg tttgaagccg
     4081 cctgccagtc gggatggggc ggaaaaccgg atgtacagac cattcgtcgt ctggtgaccg
     4141 aaagtgaagt ggcggtggcg gggaacacta aattccgctt tgtgggggct gatgccttct
     4201 cgccagatga actgcgcacc gatttgttca gcgacgacga gggtggctat gtggactgtg
     4261 ttgcgctcga tgctgccctg ctggaaaaac tccaggctgt cgctgaacac cttcgggaag
     4321 ccgaaggctg ggaatggtgc gccggacgca tggagcctgt cggtgagtgc cgtgaggatg
     4381 ccggaacata ccgcagtctg ccggagccgg aagcggtgct gacggaggcg gaagaagaac
     4441 gcctgaacga actgatgacg cgttacgacg cgctggaaaa tcagtgtgag gaatccgacc
     4501 tgctggaagc agaaatgaag ctgatgcgct gcatggcgaa ggtcagagcg tggacgccgg
     4561 agatgcgtgc cggaagcggt gtggtggtgt cctggcgtta tggcaacgtg tgtgtccagc
     4621 gtggtgtgca gttgcgcagt gaggatgacg tggctgacga cgctgaccgc acggaacaga
     4681 tgcaggagaa agcgtcagtg gaggaaatca gtctgccact gctgacgaaa atgtcttccg
     4741 aacgcacgct ggcagtccag gcggcactca tgcagcagcc ggacaaatct ctggcactgc
     4801 tggcatggac gctctgcctg aatgtgtttg gcagcggggc gtacagtaaa ccagcacaaa
     4861 tcagcctgga atgtgaacat tattcgctga ccagcgatgc gccatcgggg aaggaaggtg
     4921 ccgcattcat ggcgctgatg gcagaaaaag cccgtcttgc tgccctgtta ccggagggat
     4981 ggtcacggga catgacgaca ttcctgtcac tcagtcagga ggtgctgtta tccctgctca
     5041 gtttctgcac cgcatgcagc cttaacggtg tccagacccg tgagtgtggt cacacgtcac
     5101 gcagtccgct ggattcgctg gaaagcgcca tcggctttca catgcgcgac tggtggcagc
     5161 cgacaaaagc aaacttcttc ggacacctga aaaagccgca gattatcgca gccctgaatg
     5221 aggcaggact gtccggtgcc gcacgggacg cggagaagat gaagaaaggt gatgcggctg
     5281 aacatgcaga gcaccatatg aaagacaacc gctgggtgcc tggctggatg tgtgcaccac
     5341 atccacagac agatgccact gaacgcaccg ataacctggc tgatgccgcc tgatgaacaa
     5401 ccacaccgcc ccgccggaga cggggcggca gcaagggaga taccgtgatg aaaactgaac
     5461 tgaccctgaa tgccctgcag tccatgaacg cacaggaata tgaagatatc cgtgctgcgg
     5521 gaagcgatat gcgccgtaat ctcactcacg aggtgatgcg tgaagtggac gcaccggcta
     5581 actggatgat gaatggcgag tatggcagtg agttcggggg ctttttcccc gtccaggttc
     5641 gtttcacgcc agcccacgaa cgtttccacc tggcattatg ttcgccggga gacgtctctc
     5701 agctctggat gctggttctg gtgaattgtg gtggacagcc tttcgccgtc gttcaggtgc
     5761 aacatatctt cacgcctgtc gctatcagtc acacgctggc gcttgccgcg acacttgatg
     5821 cgcaggggta cagtgttaac gatatcatcc atatcctgat ggcagaagga ggtcaggcat
     5881 gagcgcacgt tcacgggcac tgatccccct cagcgcagag caacaggccg ccatgcaggc
     5941 tgtggctgtc accgaacaac gtcgtcgtca gggacgcaca ctttcagcat ggccttatgc
     6001 cagcgctttc tttcgctgcc tgaatggcag tcgccggatt tcgctgaccg atctccgctt
     6061 ttttgctcct gcgctgacga aggaggaatt tcatggcaac cgtctcctgt ggctggctgc
     6121 cgtggataaa ctgattgaaa gttttggtga agtctgtgtt cttcccctgc catccgatgc
     6181 ggggcatcgt ctgttcccgt ccgttccttt tcgtgaaggt gagcggcgtc gtcagaaaac
     6241 cacgctgaca gagcagaaat acagccgcca gcgggaacgt gaggcagaac gacgggaact
     6301 ggaataccag acatgttttg ctcaggcgca gattgacctt gcgtttcata ctccctccac
     6361 ggtcggaagc tggttgtccc gctggtctgg tgttgttgag gagcatgatc tggaaacgat
     6421 tttctggggg tggtgcgggc gttttccatc actgtcatca tttgaccggt ttttctggca
     6481 ggaggaacca ctctggcggc tgatttttga agctggtgag gccggtcgtg gtgcaccggt
     6541 acaggtacgt gcacttgagc agtggatgat cccgaacaag ctggagaacg caatatgatg
     6601 aaatcagacg aacaatatca ggttcccgtg tggatgcgac ctctgttgcc gttgctctgc
     6661 aataccggtg gtaatgatcc ggaggaactg ctgaatgata cagaaaccac cgccagtgcg
     6721 aatattgtcc gttatgtact gatagttgca gtgcggtcgc agattgatct gctgcagcta
     6781 ctgtacagga aaggactgtt gcgcacagag ataccaggtg gcttttcacc ggaagaagcg
     6841 caggaactcc tggataatct ggtgcgcagc catatcagca aggcgctgtc gggtgagcga
     6901 atggcagccc gtgacagaaa tgccgatctg acctggattc gccagcaact ggtcgatgcc
     6961 gcctggtttg tccgtgccac actggaagcg catgggatgg gcgtcggaaa tgagagtccc
     7021 tctgctccgc cggagacaat gccggacata cagacacggg aactggttat gttgatcaag
     7081 cgactggcat catcgctgaa agcggtgaaa cccgacagta gcgtggtgcg tgaagcgcag
     7141 gactggctgt gcgacagaaa acttgtggat atcacagata ttctccggtg aaccagacat
     7201 gtatatttta cgcagggaca cgccgatcat gatcgtttca tatcaggggt ggtctggagg
     7261 tcatgcggcg tgtcctctgc actcgccgga ataaggaagt cgccggcggc tccgctttta
     7321 cccggccatg cggggcatgg ccttgtgggt tttcagctct gtggcctcag cgtcgtgtgc
     7381 gggctgtgcc gtgcctccat cttagccggg ctggcaggga tgcaagggta cgcttcgccg
     7441 ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca
     7501 gatttcgcgg tctcaccctc caactcccgc cagccgtgtg cgtcaggctg cggcttccct
     7561 tgcgccctgt gcatccccgc cttatcgccc ggctaagatg gaggcacggc accgcccggt
     7621 gccgcagaac atgtgactat ggaggattcg ggaatgtctg ttgttgcacc tgctgtatac
     7681 gttggaacct ggcacaaata caactgtgga agcatcgccg gacgctggtt tgacctgacc
     7741 acgtttgatg atgagcgcga ctttttcgcc gcctgccgtg ctcttcacca ggatgaagcc
     7801 gatcctgaac tgatgtttca ggattatgag ggattcccgg ggaatatggc ctctgaatgc
     7861 catatcaact gggcctgggt tgaaggcttc cgccaggcac gggatgaagg ctgcgaagag
     7921 gcttatcgtc tctgggtgga ggataccggt gagacggatt ttgacacctt ccgcgatgcc
     7981 tggtggggcg aggctgacag tgaggaggct tttgcggttg agttcgccag tgataccggc
     8041 ctgctggctg acgtgccgga gacggtggcg ctctattttg actatgaggc gtatgcgcgg
     8101 gatttattcc tggactcctt cacctttatt gacggtcatg tgttccgtcg gtgatttact
     8161 cccccgctcc ggcgggggta tggcctgcca ggcccccgga aaaccctggc aggcttacgc
     8221 ctgcctgaaa tgcaggaggg ccgcgcctgc ggcgctgccc ttcctgcccg tcatggtgtg
     8281 aggcattttt ctgttatgtc gtttctgttg ttctgatttc cggaggcggg gacaccttcg
     8341 gctcccggct gcctggcggc atccggtcgt caggcccgac tccgacaggc agctgctggc
     8401 gcacccgcca gtctccgccg aacccgctgg cgcggtttcg ggctgccgct acggtccgat
     8461 gtctgaaaaa cctggcttgc agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtgac
     8521 ctgcgctccc tgcacacctg acggtgtgta aggaaactgc gcctgatggc gcagttgtct
     8581 gcccttccgc cgccacccct gcggcctgtt tcccgctgtg gactttccgg gggctgaagc
     8641 ggccacaaca cgtgtcagcc gtccaggggg agatgtacca gccacaaaac tttttgccgc
     8701 aagcgtcaaa aacttttgcg tctgcccctg gacaactgcc ccgtgccgtg gctgtcgctg
     8761 ccccatcgga aaggtccact gcggggagaa accccgaaga agtggacggc agaaggggct
     8821 ttcgcccctc cgggggagca accggtcggc ccggcgacct gattcagcag aaggagacgt
     8881 tttcatgaca gtcagcagca cgatttccgt tttttgccgc gacggggtgt tccgtaccgt
     8941 ttactgccac ctgcacggtg agccgacctg gaacggtcgc atcctgcata cccactatgc
     9001 caccggtcag ctggccgaag ccctggttga acacggagat atccgttgcc tcggtccccg
     9061 ttgcgacaaa cccgccgggc atacgcttca gaacccggtg gacggtgtga cggcttatta
     9121 cggacgtgac agtggtttcc ggatggacag tgaagcgcgt gagtaccgct ctttcaggga
     9181 ggctattgcc actgaaagca cagaagaggt gcgcttccat tatgtgttca tcgacggcta
     9241 ctggaaggtg atgtaccgca cgccggaagg ctggaagatg aaagcgctcg cgctggcact
     9301 gcgtcgctgt ccggaatgaa aaaaagcagg gcgataaacc ctgcctttct ctgccggcgc
     9361 ttccccgtac ggaagtccgg catctcattc acgaactatg gagattatgc catgagacca
     9421 tcaattatct ttgcaacggc tgaatatgta aagcgtctgc gtgaagagtg cctgcgggag
     9481 aacaaacccc tgcaccgcca tacccgcttc agacgccagg aactggcacc ggatgagatt
     9541 aacccggacg tcctggcgat gggcggtcat atcgccagac gctgcagtga gcggaagcgg
     9601 gtgcgtatcc ccgccatgaa agtcagcgag tgggggcacc tgctccgtgc gcttgaaatt
     9661 gaacgggtct gccactgatt accccctgcc gtctgtctgt gcaggtggca ggatatgttc
     9721 a
//
LOCUS       DAZUFB010000141         9493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZUFB010000141 DAZUFB010000000
VERSION     DAZUFB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.141"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..153
                     /locus_tag="ACHY3M_004366"
                     /pseudo
     CDS             19..153
                     /locus_tag="ACHY3M_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(258..328)
                     /locus_tag="ACHY3M_004367"
     tRNA            complement(258..328)
                     /locus_tag="ACHY3M_004367"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Met,seq:cat)
     gene            complement(474..899)
                     /locus_tag="ACHY3M_004368"
     CDS             complement(474..899)
                     /locus_tag="ACHY3M_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319158.1"
                     /translation="MSVMKKVLIAAIGFCLVGCAGMKLPEYSQVKASPYYTDCRAFAM
                     DVYKNDGYSKIAKTTILSMDDVKARYIVTGCVVAMGKNTVEEIKADLSAKGSSFGLIS
                     GACSSAACRVDVEQQMNAYILGSYYAANKKFPDKMKAEF"
     gene            complement(1180..1932)
                     /locus_tag="ACHY3M_004369"
     CDS             complement(1180..1932)
                     /locus_tag="ACHY3M_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS4319159.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVIAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPALTDYQ
                     WYKGYADIIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(1946..2935)
                     /locus_tag="ACHY3M_004370"
     CDS             complement(1946..2935)
                     /locus_tag="ACHY3M_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319160.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPSVRAVFRNEAVIRRAGGVECLESWLLREKGCQWLHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2943..3758)
                     /locus_tag="ACHY3M_004371"
     CDS             complement(2943..3758)
                     /locus_tag="ACHY3M_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFS4319161.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTGINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3910..4305)
                     /locus_tag="ACHY3M_004372"
     CDS             complement(3910..4305)
                     /locus_tag="ACHY3M_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319162.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            complement(4302..4628)
                     /locus_tag="ACHY3M_004373"
     CDS             complement(4302..4628)
                     /locus_tag="ACHY3M_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFS4319163.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIHSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            complement(4625..5278)
                     /locus_tag="ACHY3M_004374"
     CDS             complement(4625..5278)
                     /locus_tag="ACHY3M_004374"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFS4319164.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGKYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHITFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(5293..5376)
                     /locus_tag="ACHY3M_004375"
                     /pseudo
     CDS             complement(5293..5376)
                     /locus_tag="ACHY3M_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5373..6203)
                     /locus_tag="ACHY3M_004376"
     CDS             complement(5373..6203)
                     /locus_tag="ACHY3M_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319165.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVKEPVNEPIAVGASADESVRVRSN
                     RPEYSPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVKPETMLEGVKRYAGWVSAM
                     GNSGTQFVKQAVTFFGPDRHFEESWEVPAVSVAGREDPYFKASYDNVDYSQIPAGFRG
                     "
     gene            complement(6200..6424)
                     /locus_tag="ACHY3M_004377"
     CDS             complement(6200..6424)
                     /locus_tag="ACHY3M_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319166.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKNMKHLGGAA"
     gene            complement(6421..7311)
                     /locus_tag="ACHY3M_004378"
     CDS             complement(6421..7311)
                     /locus_tag="ACHY3M_004378"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4319167.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSGIPGRYTVQAPYKAGAGGR
                     SPELSTAIYDAPASFLSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSS
                     GGGSFPQSTEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLE
                     CSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSKPSVPSDVAHNANVLYSYISSIHQVWLQQLYPMLAKVESPLAVSLYDRINDA
                     AFLARLIHSSLNSSEVRGRK"
     gene            complement(7241..7420)
                     /locus_tag="ACHY3M_004379"
     CDS             complement(7241..7420)
                     /locus_tag="ACHY3M_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4319168.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            complement(7566..8150)
                     /locus_tag="ACHY3M_004380"
     CDS             complement(7566..8150)
                     /locus_tag="ACHY3M_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HFS4319169.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTRHVTDAIARRSNGVFVPLVVIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSNARECAAPGVVASIASGCGETNA"
     gene            complement(8178..8375)
                     /locus_tag="ACHY3M_004381"
     CDS             complement(8178..8375)
                     /locus_tag="ACHY3M_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4319170.1"
                     /translation="MRKSDVINYFGGVCKTAEALGIKHPSVSEWPEIIPEGRAYQLEK
                     ITNGKLKVDVSLYQKTNSAAA"
     gene            8471..9124
                     /locus_tag="ACHY3M_004382"
     CDS             8471..9124
                     /locus_tag="ACHY3M_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4319171.1"
                     /translation="MMNMSDRIRQRRKELNLTQQALADLTGVNRVTVTGWEKDDYQPN
                     GANLQALANALKCDPLWLVSGKGSPEPKINLKPEIFAVKKVPLISWVQAGSWTMTEPG
                     VRKEDAEEWVYTTALVSEMAFALRVRGDSMTNPLGSPSIPEGSIVIVEPDIIDTECIN
                     GKIVVAHINGGQEATLKKFVEDWPNRYLVPLNPNYKTIECGENCRIVGLVKQVIMDF"
BASE COUNT         2440 a         2514 c         2106 g         2433 t
ORIGIN      
        1 gcccctccgg agaggggcat ttttgcatgc acattctttt tcttgcatgg tgccgggtgc
       61 ctcccggtga attcagtatc agcacctgaa tccgcgatta ccccatatac ctggttgctg
      121 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
      181 ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
      241 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagcgacct ggcgattatg
      301 cgtcgctcgc tctcaccact gagctaaagg gccgggagca gaataataac tgtccgtaat
      361 taattccgaa ataaaaaacc cgctcagtgg cgggttgcta tcacagctat atatttactt
      421 attatgccgt tactaacatt tatcttcggc atataatcga aaacaaggtt tgcttaaaac
      481 tctgctttca ttttatccgg gaatttttta tttgcagcat aataactacc aagtatataa
      541 gcgttcattt gctgctctac atcaacccga catgcagcac tagaacaagc accactgata
      601 agcccaaaag aactcccttt agcagagaga tcagctttaa tttcctctac agtgtttttc
      661 cccatagcaa ctacacaccc tgtaacaata tatctagcct tcacatcatc catgctaagg
      721 atagtggttt tcgcaatttt gctgtatcca tcatttttat aaacatccat ggcaaacgca
      781 cggcaatctg tataatacgg acttgcttta acttgcgaat actcaggtaa tttcatacct
      841 gcacaaccaa ctaaacaaaa acctatcgct gctattaata cctttttcat tacactcata
      901 acctagaagc atcattgaaa ctaatttatt aaatattcat cgagtttctg gaatacagac
      961 gttacccatc tctccaaaat ctaaaagata ataagaaaaa atgtttaacg taccaatcca
     1021 tttcatagtt tcatgagaca tcaggcacaa aaaaacccgc tcagcggcgg gttcttaaat
     1081 cttatcaacg gtagacatac aaagcccatc gttgggaaaa tcttatccat attttttgaa
     1141 aaatgcaagc atcatgtcgc catcttcggc gaaaatcatt tatcttgtca cttttctcaa
     1201 ttgtgtctct gcatatgctt cttcctgcca gcactttgta accagtttat caatgatatc
     1261 tgcatatcct ttgtaccact gataatccgt cagggcaggt accagcttct ggacatgatg
     1321 ccgcgccagt gtggttggta aacggctaaa ccggttacca ttgcaacgcc cacaaatctt
     1381 ataaacaggc gtaccatgaa gccgggttct tttttcatcc aggacaatac ctttaccctt
     1441 acaccctctg cacgctgtgc tgacttctcc cttaccatga caatgctgac atagttcctt
     1501 cacccactct tccttgataa cagattcccc gcttctggag tgtttcacca cctcgcgcaa
     1561 tacattatga aatccagtac cagcacaatg ctcacagcga gccttacttg ccgcagacct
     1621 ggaataatca gcaaaggcaa aattcacaag gtaagggatg atctgtaacc gggtttcttc
     1681 actcaattta ttcaatgtcg ggttatccag tgccatcgcg taattgagca gaccttcaat
     1741 cgcaaactga ggatcctgaa caccaacttt tgccaggaat aaggcaaaac ccagtggtgc
     1801 tttcgactgc accatcccct gcgcagcaat tacatccgta attgttaaac caccagagcc
     1861 tgtcgccggt gcgtcatcac tcagttttgg agattttggg gagtaatatt ttggtaaggc
     1921 ttcaaggttc atgctcgttc tccacttacg ccagtacgcc tattgccagc gcacgatcga
     1981 taaaacgaaa tatcagctcc agctgggagc catacttctc ttcaaatgtc acggtatccg
     2041 catgcagctc gtcgtgatgc tttctgcaca aaggcaacac aaagagatca tgcgcttttg
     2101 tacccattcc accctgaccg tggcctatca ggtggtgggg atcatcagcg ggctttccac
     2161 aacatgcgca cggctgcgtc ttaacccagc gcgtgtactt ttcattaacc cagcggcgac
     2221 gtttggggcg taacataaaa gactccggcg actccggatc cactttcagc gccagcacct
     2281 ttttcgcttt atcctggatg atgctggtgg caggaaccga aggcacaagg tcactttccc
     2341 gggtgacaga cggcacaaca ggcttcggta atctcagtgc cttacgggct gcactttccg
     2401 gtaaggcatc cgccaggtca ttacgaatca gccaccagca cagttccggc attgtcacaa
     2461 cgtgactgtc atcaaaaccg agatcccgac gcacaacaga caacacccag cgggcacagt
     2521 tatccgttgc cattgattcc agccgttccg tgaactgatc gcgcagctgg ttatcgcagt
     2581 gccagcacag acggattgca cccggagcgt gtcgcattgt ggtcatgttc tcgctgtgcc
     2641 agtcggaatg aagccactgg cagccttttt cacgaagtaa ccagctttca agacattcca
     2701 cgccaccagc acgacggatc actgcctcat tgcggaacac ggcccgaacg gaaggatcat
     2761 ccgccagcgg ttgtgatgcc gccggaacgg caccactagc gaaagatgaa taacgttccg
     2821 gctcaggctc cagcaggaca cgcccctgca taaacagggg catcagctct gaacctggcc
     2881 tgaacaatac gatccccata cgcggggcaa tttcaggggt cagtagtgct ctcacggtca
     2941 cctcaatgaa cggtatcgag cagctttaac agctcaggga atcgggattc gaagaaatgc
     3001 ggctgcgtct cgcgcggatt tgcgggactg gtgatgttct tgccgaacat gcagcctttc
     3061 gccgtcagcg accagaattt tttgatgttg ttaatcccgg tacggctgta tcgttcgcgc
     3121 tgctcgacga tccccagctt caccatctgg tgatatgcct gattagccgt caggcggata
     3181 ccatactgct tcagcagtgc actcagcgac agcgtagggc ggcttgaacc atctggcgca
     3241 tcagcaggtg catcaatggc atagatcggc ataagttcag gaagaccagc tacctttgat
     3301 aatttctggt atgcaccaag tttcgaggag tttgacagat ttagagtctt tgctgctgat
     3361 tcaagcagaa tgaccccgga tttaattttg tcggatgtgg tttcttctgg tgatgaatta
     3421 tgaagcgcat caaaagtacg tatcactttt aagctgaatg ccgggctgat ccacattgca
     3481 tatgcataga ccagctcttt acagacatac gtcccaccat tgcgcccctg aatggtgatg
     3541 acaggaatac tacgggaatc tcccgtagtt tcttcttcca gtaattccac aagagccttc
     3601 gtttcaggac gacgcataaa ctcgtgaact tccagcgaac gggaggagcg attctcacca
     3661 gcggcaagaa gagcagcttt ctgaaggtcg ttaagacagt agttagattc aaagtactgg
     3721 cgcacagaaa cgccatcaat tacaagcaac tgattcattg gtttctccac aaatttcggg
     3781 actgcactcc cttttcgttg atgcaagatg aacttactgc gatttttaat agttatcaag
     3841 gatacactgt tcataaatac agtatcttta acgaggtaat acccaaattt agggtgttgc
     3901 tcaattccgt taccgagttg ctaatttgca actcgctttt tcgtacttac tgatagtgat
     3961 ctcgaccttc ccttccggga taaccggtcc ccactccacc agcattcttt tcacctgact
     4021 gtcgtcttcc cacacacccg cgtgggtcag ggcgtcaaac agcgccttgt tatagttgtc
     4081 cagatcgcgg atccggttat ccggaggaaa caacacgatc tccactgatg caggtgccga
     4141 cgttggtttt ggcagacgac gtaactgctc aactattgct gcacacgccg cgctctggaa
     4201 tttgcgcccc gccgcgctta tcaggctctt accagcaaac gcccctttgt tggggtgtcg
     4261 ccagtacgtg ttcacgctgg gcggaaaagg caggatcagc ttcatacttt caggcccctc
     4321 tcatgtaacc agtgggttgc acgcagcctt gcgttttcct caccggcaag cagtgagtgg
     4381 ataatcccga ccgcctcgct gtcgtcgtcc ttcaccgcgg tatgaagcgt tatcccccgg
     4441 gccacgccac gctttatcgt gatgacgcct tttttctcca gtgcgcgaag atgctctacc
     4501 gctgcattca ctgaacggta tcccagcatg gttgccacct cctgattggt tggcggaaag
     4561 ccacgctctt tctgataaga aatcagcata tccagcacct gctgctggca ttgagttaat
     4621 gtcgtcatgc cgccatctcc ctgaccagtt tttccgcctg ctggcgaacc tgcgccagaa
     4681 acgcctcacc acatgcctca agttcatcgc gcccaatgta gctgattgcc ggtcccttcc
     4741 aggtcttgtc aaaaacagca atagcaccag cgaaaaaagc tcctgtcggt acctgcttct
     4801 cgtctttcgg gataaaccag acaggcagtt caaaaccaat acgcccgcga ataaaagtaa
     4861 tatgatctgc atcttccggc caccacactt cgctggtggc agctttgatc aggaaaacat
     4921 agcgcccgcc tttatcacgc atggcactgg catgcttcat gatgtaacgc atgccagtga
     4981 tgtatttccc ctcatgctga ctggcgcggc tgtatggggg attaccaaag gcagcacctt
     5041 taagctccgc aagacgttct gaccagtcat gcgccagcgc gttatcttcc gccgtgtaat
     5101 acgcggcaca tttggcgtta tcaccgtcag taaacagatc cagaacaaac gggccaaaca
     5161 gggtgttaat tccccagaaa atgttgtccg gcgtgcgcca ctgatcgccc acttccttca
     5221 gttcatgggc tggtttgttc cgcagttcta ccagcgcctg gcaatattta ttactcatta
     5281 agcccccacg taaaaagcat ccgcaatgtc tccggaagta cagcccggat gggcttcaat
     5341 gaatttctga acgtcattta acagactcat gatcaccccc tgaatcctgc cgggatctgg
     5401 ctgtagtcca cgttgtcgta actggctttg aagtacgggt cctcacgtcc ggctacagat
     5461 accgcaggaa cttcccagga ttcttcgaaa tgacgatccg gaccaaagaa cgtgacagcc
     5521 tgtttcacaa attgtgtgcc gctgttaccc atcgcagata cccagcccgc gtagcgtttc
     5581 acaccttcca gcatggtttc gggttttacc ccctcattca aacgggcttt ccaggctttg
     5641 aaggctgcag attttgaatt gccaccagca cgtttggggt atgccagcca tgcctgctca
     5701 aactccggag agtattccgg tcggtttgaa cgaactcgca cagactcatc agcagatgca
     5761 ccaacagcta ttggttcatt gactggttct ttgactggtt ctttgactgg ttcaaaagag
     5821 tgactggttc tgggtgaatc tcctgcacta ccccctggtg caactcctgc actacctggt
     5881 gaatttgctg caccagatag tgaattattt gcactacccc ctagtgaatc tcctgcacca
     5941 tccagatgaa ggagatagat attacttgag ttaccttttt cacctttccg ggtgactttt
     6001 tttaccagcc cggactcaca aagggccgca atatgattca tcacagaacg tttgctaatc
     6061 tcgcactggt cagcaatatg ctggtagctg ggccagcact cgccctgatc gctggcatta
     6121 tcagccagct taatcagaac cagttttcgc aatggatttc ccactcgaat tttcatcgct
     6181 ttaaccatca gctccatact catgcagcac ctccgagatg cttcatgttt tttccggagc
     6241 aaaaggctat aagcggcata ctgatgcggt aattacggcc cagcggttca caaatcacct
     6301 tctgacattc acggtcaacc aggctaacac gtagaacatg ccctgcaggt gtggtgtacc
     6361 actgaccggg gcgaggacaa cggaaagtct gattggtaaa ccgtttgaaa atattccgga
     6421 tcatttacgc ccccttacct ctgaagagtt cagcgacgaa tgaataagac gggcaagaaa
     6481 tgccgcatcg ttaattcggt catacagact tacagccagc ggtgattcaa cttttgccaa
     6541 cataggataa agctgctgca gccagacctg atgaattgat gaaatgtagg aatagagaac
     6601 gttggcgtta tgtgcaacgt cgctcggtac agagggcttt gaaagctgtt tctccatctg
     6661 gttaaaggca ttgatgtatg cctctttgaa ctgggcagca cgtttaccag tgaaacccat
     6721 agcaagaaac gcaaagccgt cgcgggttat ttgatagcaa ggtagtttgc ggcctgtgca
     6781 atcggtgtaa tcactcaccg aaaaattgcg ggcagtgaat gatgcggaac attcaagcgt
     6841 gcggatcttt ttcagtacat cgtcatgacg tttggagaag aagttggcaa cagccaggga
     6901 tgaagtaaca gcctgaccat caacgatggc aatttcaggt tgagtgaggg ttgggatcgt
     6961 agccatgatg gcagcctccg ttgactgtgg aaaacttcca ccaccggagc tgcgaaactc
     7021 actggtggca gactgaacag ggttcgcagt accggcgtca acggagaccg gcgagccttt
     7081 cggctcccct gcccagccca ccataattct ggcgtgcgtg agcgcggacg ataaaaaaga
     7141 cgctggcgcg tcgtatatcg ccgttgacaa ttccgggctg cgacccccgg cacccgcttt
     7201 ataaggtgcc tgaacagtgt aacgtcccgg aattccagaa tcaatatgct ggtggtcctt
     7261 cacactcaac aaaatcacgc ctgaatttcc acaaaggact aaagcactca tgtgggtagt
     7321 ctttgcgaag atagataacg cgctgtgttt ctggctccca acgaataaca tgaacataaa
     7381 gtcctcttcc gtcacgaaac cagcggttaa gttcctgcac aactcgcccc ccacagtcag
     7441 gtaaagttct ctgtggttac ttacagccag gtgatttggt aatctgcatt catgccgtaa
     7501 caacaggtgt tcagagacgc tgaccaccag ctgttgcgac aaacggttat ttgccgttaa
     7561 actgttcatg cgttagtttc tccacaacca gaagcaatcg acgccacgac gcccggagct
     7621 gcacactcgc gggcgttact cttttccggc gcacaaaaaa cacgaaataa cagtgttaaa
     7681 tgctcctgcc acttcgccat tacttggtag ctgttctctt cgatttgctc acgctcagct
     7741 tggtcaataa ctccatcagc agttgccttg cgtaagtact gggaatgctt gccaatccat
     7801 tctattgact ccatcagccg ctgattaatg tcaccattgt caatgtcatc aatgaccacc
     7861 agcggcacaa acaccccatt actacgacgg gctattgcat ccgttacatg cctggtacca
     7921 ctggcatcct gtaaaaccat ggcccactca agtggaaaaa tttgatcccc accgctacgc
     7981 agtctgttat gcaattgatc ttttgctggg gtgatatcat cagatttata caaaccaaga
     8041 atttctgctg cttcctcata gccatgaggt aaatcagcaa tcgttcttcg tattgctgcc
     8101 accagccatg ctggttgctt atcaactttc cattcaggtt ctttacccac gtttaagccc
     8161 tcatatctgt ggtgttttta tgccgcagca ctgttagtct tttgatataa agacacgtca
     8221 actttcagtt tcccgttagt aattttttct aactggtacg ctcggccttc aggaataatc
     8281 tcaggccact ctgaaacaga cggatgctta atacctaggg cttcggcggt tttacaaact
     8341 ccgccgaaat aattaatcac gtcggatttc cgcatttctg tctcccgtta aattacgtta
     8401 agcaggaatg taggatatcc aacatatcaa tgtcaagaat cctacatgag catgtggtag
     8461 gattgcctac atgatgaaca tgagtgatcg tattcgccaa aggcgaaaag aactgaacct
     8521 gacacaacaa gcactggctg atttgactgg tgtgaaccgt gtcacggtta ctggatggga
     8581 aaaggacgac taccaaccaa atggagccaa ccttcaagcc ctagccaacg cacttaaatg
     8641 cgatcctctg tggcttgtta gcggaaaagg ctcgcctgaa ccaaagataa atctaaaacc
     8701 tgaaatattc gcagttaaaa aagtccccct catctcgtgg gttcaggcgg gttcatggac
     8761 aatgacggag cctggtgtca ggaaagaaga tgctgaagag tgggtttata ctaccgccct
     8821 tgtatcagaa atggcatttg cactacgggt ccgtggtgat tcaatgacca atcccctcgg
     8881 ctcaccatcg ataccagaag gttctatcgt tatcgtagag ccagatatta ttgatacaga
     8941 gtgtattaac ggaaaaatcg ttgttgccca tatcaatggt gggcaagaag cgacactcaa
     9001 aaaatttgtt gaggactggc cgaacaggta tctcgtccca ctaaatccta actataaaac
     9061 tattgaatgc ggtgagaact gcagaatagt tggtcttgtc aaacaagtaa taatggattt
     9121 ttgacacatc ttcctcacta tcgcaaaacc ggggtatccc cggttttttt atgagcctat
     9181 ctttttatgt aggataacca acataaactc ttgacactca catgttggat atcctacatt
     9241 tgtttttaga gttgtggtga atgcgcaggc tgatgcgcga aagacattgc agctattgcg
     9301 gaaaagagct gttcggcggg gcaattaaac gcccgtgaga gtctgaaata accgcaagcc
     9361 ggagatcagc accggtcacc acaacagcca ctgctttggc ggtaccagtt tgtacacttg
     9421 cttccggctg gtaccgctct ttttacaaaa cagagaagag catcaccgga cgacgggctc
     9481 ataacccaat cca
//
LOCUS       DAZUFB010000142         9425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZUFB010000142 DAZUFB010000000
VERSION     DAZUFB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.142"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..368
                     /locus_tag="ACHY3M_004383"
     CDS             120..368
                     /locus_tag="ACHY3M_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4319172.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            346..1476
                     /locus_tag="ACHY3M_004384"
     CDS             346..1476
                     /locus_tag="ACHY3M_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4319173.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1522..2160)
                     /gene="ompW"
                     /locus_tag="ACHY3M_004385"
     CDS             complement(1522..2160)
                     /gene="ompW"
                     /locus_tag="ACHY3M_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFS4319174.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2400..2489
                     /locus_tag="ACHY3M_004386"
                     /pseudo
     CDS             2400..2489
                     /locus_tag="ACHY3M_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2517..3260
                     /gene="yciC"
                     /locus_tag="ACHY3M_004387"
     CDS             2517..3260
                     /gene="yciC"
                     /locus_tag="ACHY3M_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFS4319175.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3290..3829
                     /gene="yciB"
                     /locus_tag="ACHY3M_004388"
     CDS             3290..3829
                     /gene="yciB"
                     /locus_tag="ACHY3M_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFS4319176.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3934..4332
                     /gene="yciA"
                     /locus_tag="ACHY3M_004389"
     CDS             3934..4332
                     /gene="yciA"
                     /locus_tag="ACHY3M_004389"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFS4319177.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4372..5091)
                     /gene="tonB"
                     /locus_tag="ACHY3M_004390"
     CDS             complement(4372..5091)
                     /gene="tonB"
                     /locus_tag="ACHY3M_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFS4319178.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5182..5472
                     /locus_tag="ACHY3M_004391"
                     /pseudo
     CDS             5182..5472
                     /locus_tag="ACHY3M_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(5478..5713)
                     /locus_tag="ACHY3M_004392"
                     /pseudo
     CDS             complement(5478..5713)
                     /locus_tag="ACHY3M_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5884..6171)
                     /locus_tag="ACHY3M_004393"
     CDS             complement(5884..6171)
                     /locus_tag="ACHY3M_004393"
                     /inference="COORDINATES: protein motif:HMM:NF018156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFS4319179.1"
                     /translation="MFILVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSRVCSLY
                     DEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            complement(6159..6533)
                     /locus_tag="ACHY3M_004394"
     CDS             complement(6159..6533)
                     /locus_tag="ACHY3M_004394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319180.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSF"
     gene            6765..7821
                     /locus_tag="ACHY3M_004395"
                     /pseudo
     CDS             6765..7821
                     /locus_tag="ACHY3M_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(8040..8630)
                     /gene="espM1"
                     /locus_tag="ACHY3M_004396"
     CDS             complement(8040..8630)
                     /gene="espM1"
                     /locus_tag="ACHY3M_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFS4319181.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNTTTDYI
                     ANVFADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            8814..>9425
                     /gene="nleG"
                     /locus_tag="ACHY3M_004397"
     CDS             8814..>9425
                     /gene="nleG"
                     /locus_tag="ACHY3M_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFS4319182.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFC"
BASE COUNT         2584 a         2188 c         2077 g         2576 t
ORIGIN      
        1 aaaatacgtt tcagttatct ggcaaaaact gatcccgaat caggctgatt tttaatgttc
       61 aaccccggtc gttgcccacc agctatagtg gcggcgacca tgattagcga acgacgctca
      121 tggcaagact tattctgctc actgagtggg caaaagagga attcagtgaa ccggtcccaa
      181 ctccgagtac gttaagtaaa tacgctaaag ccggaatgat atttcctctc cccaaaaaag
      241 ttggaagacg ctggcgagtg gatccgcaag ctcgctttgt cggaatggta aacaagccgg
      301 aggtgatcgc cacagatcac cctgctttga agaggatact ggaagatggc gcgcccgcga
      361 aaatataaaa ccgatgttcc gggattatct ccgtattttg acaaaagaaa taacaaagtt
      421 tactggcgtt acaggcatcc cataacaggc aaaaatcacg gtctcggcag tattgaccag
      481 aaactggcag aaactattgc agcagaagcg aacagccgtc ttgcccggca gcaaatggaa
      541 caaatgctca gtctgcagga gaaaattatt agtgataccg gcggttcatc aaccgttacc
      601 atttttctga ataattacag aaaaattcaa caggaaagat atgaaaacgg cgagatcaaa
      661 ctcaacacgc tgaaacagaa agcggcccct ctcagggtat ttgatgaacg ttttggcacc
      721 agaccgttag atgccataac cgtaaaagat gtggtatcag tactggaaga gtacaaggcc
      781 agaggacata acagaatggg acaaattttc aggaaagtac tgatcgatgt tttccgggaa
      841 gctcagcaaa cgggcgatgt cccgccaggc tttaaccctg cagaatctgc aaaaaaaccg
      901 caggtgcgga tatcaagaca gcgactgact tttgatgagt ggatgatgat ttataacgca
      961 gcggaaaagg atggttactt tttacagcgc ggtatgctgc tggcactgat gacaggccag
     1021 cgcctttcag atatttgcaa aatgcaattt tcggatatcc gggatggtta tcttcatgtc
     1081 gaacagcaaa aaacaggaac ccggattgcc atccctctgg ctctgcgttg cgataaatta
     1141 aatctcaccc tggatgatgt ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta
     1201 ttgcaccacc atcacgcgaa agggacagct aagcgcggcg ggatggttaa gccagcaaca
     1261 ttaaccgttg catttaaaaa agcccgggat tctgtggatt acaactggcg tgctaatggc
     1321 acccctccct ctttccatga gcagagatct ttatcagagc gattgttcag agagcagggg
     1381 gttgatacca aaattttgct aggccattcg aatcaaaaaa tgaccgatat ttacaacgac
     1441 gcacgcggta aggaatggaa aaaactggtc atttgatgac cagttttgca gaggggtttt
     1501 gcagaggttt tgcagagaaa attaaaaacg atatcctgcg gagaacataa acacccacgg
     1561 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1621 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1681 ccccacctgc ccggcagctc cccaggaatc tttcagactg agatcggaaa gccccgcctc
     1741 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac cggccccaac
     1801 gtaaggacgg aatttgctgc tggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1861 cagatggtga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1921 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaagcc
     1981 cagttgagtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     2041 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     2101 aaaggcactg ccagaaagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     2161 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     2221 tgatcctaca tagatcacat tacatatgat aattcaccat ttattgatct ggattaattt
     2281 agaagttgca ttgagaatcc tggttaatgt gatttaaatc aatttttaaa tattttctct
     2341 acgcaaatat taactcgatc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     2401 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     2461 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     2521 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     2581 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2641 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2701 ggttgttcga cctggttcag aatatgtcac cggaacagca acaaattttg ctgcaggctt
     2761 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2821 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2881 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2941 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     3001 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     3061 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     3121 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     3181 tggcgaacac cttgagcaac ctgatttctg ccatattgct catctatctg ttccgcctgt
     3241 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     3301 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     3361 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     3421 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     3481 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     3541 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3601 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3661 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3721 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3781 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3841 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3901 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3961 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     4021 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc gaaagaaatt
     4081 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     4141 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     4201 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     4261 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     4321 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttctg cttactgaat
     4381 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     4441 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     4501 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     4561 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4621 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4681 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4741 tacgggtttg atatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4801 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4861 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4921 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4981 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     5041 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     5101 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     5161 attgcatggg agtttatcac cgtgagaacc gtaataacat accttcggtt cagttcagct
     5221 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa atgacctgtt caagcaatgg
     5281 ttgaagaaga atagcgatgc tcaagtagta gcgtctttca gtgatgaggg tctatccggt
     5341 tacaaaggta agcatcttac tggtcagttt ggtgacatgt tagcccgtat tgagtctggg
     5401 gagtttccag aaggtacaat tctgctagtc gaagctatcg accgcatagg ccgccttgaa
     5461 catcttgaaa ctgtttttca aaacagttcc gtgctgagac agccacaagt actgtgtcgg
     5521 caccaccgat tgtaatcaca ctagatctaa ttaaacacag ccgtctgcat tatttcccga
     5581 ctcataaaat attagccgta aatgcattcg acttttagtg agtggaagaa actttccgcc
     5641 ctagcattat cgtaacagcg cccctttgca ctcatcctgt catgcaggta tgccgcttca
     5701 gaagcgcctg ataatgcgtt gaaatcaggg cggtggctac ttgctattca gttcacgata
     5761 caaaatgggc aatatccctt aaggaccagc gcatagcaat gagtcacttt attatcaagt
     5821 ttgatgaccg ctttgatgct aacttttaat aagagtctct tcacacaaaa tcgtgtaccg
     5881 agtctacact gaataacaat cactcctcac cgtaaagtga ccgatactgc aatcaaactc
     5941 acactgctct ctggccacaa gcattgataa tgttattggc tctctgctta gtgggtgatg
     6001 catcccatca tttattatac gagaaagagc catttcatca tataaactac atactctgga
     6061 accttcagca tttcttaaaa aaacacctcg ttgaggaatt gccagagtaa tcgggcactg
     6121 taatgcttca tctatgtaag acatttcttc aatattaact aaaatgaaca tgaatgtaat
     6181 cgccgctgta acaattcaat tctgttatca ttactatgca taaattcaga aataacctgc
     6241 cccatatatg tactgaatac atcaagaaat gaattgcctc cattcaattg attttcaaca
     6301 gaatcagcca atctaatgat atcggctagt tctgtatggc ttaaacccga atatagttcc
     6361 ccttgaacag gatctacaat aaaagcgtcc atagcactct gatagcgaac agaaaacgat
     6421 ctttctccta ttgtgagtct ttccatttga tgatctctgg tcgcacgttg taaaacttca
     6481 acatcttgtg ttgacaagta atttccagaa gacaaattaa atgaaaatgg cataatttta
     6541 tccctaaagc atatatctta cttttcgata attaacaaaa agccatcctc cacagcaata
     6601 ctccatcaga ataattgtag gattatttta atctaattta taagcataaa atatatactc
     6661 actaaatagc tgcgcagaat agtagatcat ttagagggaa ctcaacccga ttgtgcgatc
     6721 tgatcaatcg ccaaatcaat acaaatcacc aaccggactg agcaatgtcg atcataccac
     6781 caatttcccg tgacgaacga cgcctaatac agaaagccat ccataaaagg ctgacagcca
     6841 tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc ccgcacacta tgctgcgccc
     6901 gttcctctgt tgggcgctgg attaactagt tcacacagtc gggtgttgag ggactgaaat
     6961 cattacctgc cgagcgagcc cgccgctggc cgtttgcgca tatctacaca ctattacgtg
     7021 aacaggtaaa atattcactc gcgactttgg ctacaattat tcacgctgga gtacagaact
     7081 gctgacaata aaaatcaatg agataaccgg ctgcctgtta catgctggaa ccattcgccg
     7141 ctggttgtcg tctgtggggc ttgtgtggcg aagggctgcg ccaactctac gtatccgtgc
     7201 cccgcctaaa gataaaaaga tggcaacaat ccataacgca ctggacgaat gcagcacaga
     7261 gcatccggtc ttttatgaag atgaagtatt tatccatctt aatcccaaaa tcggtgcgga
     7321 ctggaaactg ctcgggaagc aaaaacgagg ggtcacgccg gaacaaaatg aaaaatattc
     7381 tctggacgta gcgctgcaca gcgggacagg ttaagtcagc tgtgtgggcg ccaacagcaa
     7441 aagttcggcg ctgttcatca gactgctgaa gcggcttaaa gctacatact gtcggacgaa
     7501 aaccatcacg ctgctcgagg acaactacat tatccacaaa ggccgggaaa cccagcgctg
     7561 gctgaaggat aaaccgaagt gcagagtcat ttatcagccg gtttaatctc catgggtgaa
     7621 tcattttgaa tagctatggc agatacttca cgccacaata acgcgtaatc atcagtgccg
     7681 ctcaatgtgg caactattga aaaagttcgc cattttatgg aatccgtcag tccattcccc
     7741 gaaggcaaac atgggctggc aaaaatgtgc gatattaggc gcagctattt agttattccg
     7801 tcatttctcc cgcccgtgta aacactactg aatcttgtcg ttgtgacaaa gctataaata
     7861 gtcaatcaca gcacgtttct gcgtacgacg cgctgcaatt ttctttacga tttataactg
     7921 tacaggtgaa ctatgagtta tttggataca tacatatagt gtaacgcctg ttgtatattt
     7981 tactcatatc tttttacagg atgaaaacca atgatactgg cgcatagcct cctccaacct
     8041 tacccctgta taacacgact catataacct gggatttcat ttcgtagttt tacaatatca
     8101 acacccgggg cgatatgttt ctgataaaac ttgtctgcaa acacgtttgc tatgtaatct
     8161 gttgtagtgt tttttacagc tcggctcata ccatcgcaaa gagcatccat ttttttgcca
     8221 aaagaagcat ttcctattat tatatgatta atagaacttt gtgccgcttt aacatcaaga
     8281 gttagttcaa actccttact tacaagagaa aagaccattt tcctctcttc agaagaaatt
     8341 tttgcatttt tttgaaggca gcaattatca atctcttggt tgataaccct tgacggatat
     8401 actgtccgtt catcacttac ccatccatga atatctttag tactctgcct gcttaccgtc
     8461 tccagtatac agccgctaaa tttgttaaca ttaaagcgga tagagcgctc tcccattgaa
     8521 cacttcacga cctcatcatt cttcccacgg atggtgcccc ggaaagaatt atctttatga
     8581 taactgatac caaaagagct gaaggacaca cctgtcgcat ttactggcat aacaaatcct
     8641 tttcattaaa atttgcttac aaaaattgta tacaatcatt ttaattaaaa aaatacatgt
     8701 gtaaatagca ttcgttcaaa atttaatttt ttgtttgaat cctatcacat atttagtatc
     8761 ttgactcctg tcaccaatat ggttcaataa catagactaa agagggagaa aatatgcctg
     8821 tcatattaaa cttttcgagt gaaagagtat tgtctgaaag tgaactggag gctttacgtc
     8881 atgtcggaag agttagtcag agtgaacagc ttgttgtgag aggccgcact atgagacttc
     8941 atcatatttc atttatggac agttttagcg tggaaccggt ttccggcgga ctgttggatc
     9001 gccttagtgc aagagggcat cgccttctcg cagaaaatct ggagatacag cttaacagag
     9061 gccatacttt tttgcaggca ttccgtttat atatggaaca gagccgtgca accccatgta
     9121 cgcgccaaaa cgtcagtagt gcaatacaaa acaaaattaa ctctcatgca tttactgtta
     9181 gtcatcaaga cttttcctgt catgaacaac acctgaattg cccgataaca ttatgcattc
     9241 ctgaaactgg tgtttttgtc agaaatgcaa aaaactcaga aatatgttca ttatatgacc
     9301 acaatgcact cactgaactt atccgacgca acgctccaca ccctctcagc cgtgaaccct
     9361 ttgttccgga aatgattgtc agcaaagatg aatgccattt taatctaata gaacaatatt
     9421 tttgc
//
LOCUS       DAZUFB010000143         9278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZUFB010000143 DAZUFB010000000
VERSION     DAZUFB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.143"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            419..925
                     /locus_tag="ACHY3M_004398"
     CDS             419..925
                     /locus_tag="ACHY3M_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4319183.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDAGFCIF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
     gene            897..2825
                     /locus_tag="ACHY3M_004399"
     CDS             897..2825
                     /locus_tag="ACHY3M_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4319184.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2809..3015
                     /locus_tag="ACHY3M_004400"
     CDS             2809..3015
                     /locus_tag="ACHY3M_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFS4319185.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            3012..4604
                     /locus_tag="ACHY3M_004401"
     CDS             3012..4604
                     /locus_tag="ACHY3M_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4319186.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4594..6099
                     /locus_tag="ACHY3M_004402"
     CDS             4594..6099
                     /locus_tag="ACHY3M_004402"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4319187.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRVDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSAIKAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSIEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVRGGVSQTSADGDADVKMLMAMP"
     gene            6136..6483
                     /locus_tag="ACHY3M_004403"
     CDS             6136..6483
                     /locus_tag="ACHY3M_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4319188.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            6541..7569
                     /locus_tag="ACHY3M_004404"
     CDS             6541..7569
                     /locus_tag="ACHY3M_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4319189.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGMLVLG
                     NTAAEGIRCYGAIKDAQALSEGVVASSRYPKHWLTVGDPSCEFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7621..8004
                     /gene="gpFI"
                     /locus_tag="ACHY3M_004405"
     CDS             7621..8004
                     /gene="gpFI"
                     /locus_tag="ACHY3M_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4319190.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            7997..8350
                     /locus_tag="ACHY3M_004406"
     CDS             7997..8350
                     /locus_tag="ACHY3M_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4319191.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8366..8899
                     /locus_tag="ACHY3M_004407"
     CDS             8366..8899
                     /locus_tag="ACHY3M_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319192.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            8896..>9278
                     /locus_tag="ACHY3M_004408"
     CDS             8896..>9278
                     /locus_tag="ACHY3M_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4319193.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT         2160 a         2166 c         2850 g         2102 t
ORIGIN      
        1 cccatattca gtgttgctga tttgtattat ctgaagttgc ttttacgtta atttgatgca
       61 gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg atggcgtaga
      121 tgcacgttgt gacatgtaga tgataattat tatcatttta cgggtccttt ccggcgatcc
      181 gacaggttac ggggcggcga cctcgcgggt tttcgctatt tatgagattt tttgaggggg
      241 agttgttgtt taattgtttg gcatatctaa ttgataagta aggtgaaaat aaaataaata
      301 caacaacctt acgatgtgtt ttgatgtcgt caatgcgaaa aatgtcaatg atatcaaatg
      361 gttttgtaaa aacacatggt tgttgtatcg ctttttatcg atggcttatg gagaggagat
      421 ggccttttta ttgaataaaa gtgatatggc ctcctccatc ggtatatctg ttcaggcatt
      481 tgataaatgg ggcgttcctc ctgttgagcg tcgggggagg gaagtttttt atgacgttaa
      541 aactgtactg gagatagatc gcgagcggcg acaacacaat cagagaatac ctgatgacga
      601 gggcgatctg gaggaaaggc tgcttcgggc cagagctgaa ctgacagaag aacaggccgt
      661 agctcaaaaa cttaaaaatc aggtaaccga aggtaagctt attgacgccg gattctgtat
      721 ttttgccctc agtaagctgg caatggcgtt atccagtacg cttgattcca tccctttatc
      781 catgcagcga cagtttcctg atttaacacc gcgccatctt gaccatctga aaacccttat
      841 tgctaagggg gcaaatcagt gtgcgcgggc aggggataaa ttaccggatt tacttgatga
      901 atatatcaga gcaacaactg aataatatga tgagcgctgt cacaactgca ttacagcccc
      961 tgataagggc attgccggtg acgccagttg aatgggctga tcaaaattat tatctgccta
     1021 aagaatcttc atatggtgag ggcgaatgga aaacgctgcc attccagatc gccatcatga
     1081 acagcatggg gaatgatcag atccgcactg ttaatctgat taaatctgcc cgtgttggct
     1141 atacaaagat gttgctgggg gtggtcgggt attttattga gcataaatcc cgaaacagtc
     1201 tgctttttca gcccacggat tctgccgctg aagattttat gaagtctcac gtggaggcga
     1261 cgattcggaa cgtgccatgc ctgaaagacc tttccccatg gctgggtcgt aaacatcgtg
     1321 acaatactct cacgctgaaa cgcttttcat cgggcgtcgg tttctggtgc ctgggcggcg
     1381 ctgccgccaa aaactaccgt gaaaaatccg tggacgtggt ctgctatgac gaactttcct
     1441 cgttcgagcc ggatgtcgaa aaagagggct cgccaaccct gctgggggat aagcgtattg
     1501 aggggtcggt gtggccaaaa tccattcgcg gctcgacgcc taaaatcaaa ggcacctgcc
     1561 agatcgaaaa agccgctaac gagtcggcgc attttatgcg tttttatgtg ccctgcccgc
     1621 actgtgggga ggagcagtat ctgaaatttg gcgatgagtc cacgcctttt gggcttaaat
     1681 gggagaagga cagccctgaa agtgttttct acctctgtga acatcatggc tgcgtgatcc
     1741 atcagtctga actggaccag agcaacgggc ggtggatctg tgaaaacaca ggcatgtgga
     1801 cccgtgacgg tctgacgttt ttcagcgccc ggggtgatga aattccgccg ccgcgctcca
     1861 tcacgttcca tatctggacg gcgtacagtc cgttcaccac ctgggtacag attgtctatg
     1921 actggctgga tgcactgaaa gatcccaacg gcctgaaaac ctttgtgaac accacgctgg
     1981 gcgagacctg ggaagaggcc gtgggcgaaa aactcgatca ccaggtactg atggataagg
     2041 tggtgcgtta cacggcggcg gtgcctgccc gggtggttta tctgacggcg ggcattgact
     2101 cgcagcgaaa ccgttttgag atgtatgtct ggggatgggc tccgggagag gaagccttcc
     2161 tggtggataa aatcatcatt atggggcgtc ctgatgagga agagacgctg ttacgtgtgg
     2221 atgcggcgat caacaaaaaa taccgccatg cggatggcac cgaaatgact atttcccgtg
     2281 tctgctggga caccgggggg atcgatggtg aaattgttta tcagagatca aaaaaacacg
     2341 gtgttttccg ggtgctgccg gtaaaaggcg catctgtcta tggcaagccg gtgatcacca
     2401 tgccaaaaac ccgcaatcag cggggcgtgt atctgtgtga agtgggaacg gacaccgcaa
     2461 aagaaattct ctatgcccgt atgaaagccg atcccacgcc tgcggatgaa gccacgtcgt
     2521 atgccatccg ttttcctgat gatccggaga ttttttcgca gacagaggcg cagcaactgg
     2581 tcgcggaaga gcttgtggag aagtgggaaa aaggaaagat gcgtctgctg tgggataaca
     2641 aaaagcggcg taacgaagcg ctggactgcc tggtgtatgc ctacgcggca ttacgtgtgt
     2701 ccgtgcaacg ctggcagctt gatctggctg tactggcaaa atcccgggaa gaagagacga
     2761 cccggccaac ccttaaagaa ctggcagcga agctgtccgg aggagtgaat ggttacagtc
     2821 gctgaactgc aggcgctgcg tcaggcgcgc cttgatttat taaccggtaa acgggtggtg
     2881 tctgtccaga aagatggtcg cagaattgaa tatacggcgg cttctctgga tgagcttaac
     2941 cgggcgatca atgatgcgga gtcggtactg gggacaaccc ggcgtcgccg tcgtccgctg
     3001 ggagtgaggt tatgaaacga acgcctgtcc tgattgatgt gaacggcgtt ccgcttcgtg
     3061 agagtctcag ctacaacggg ggcggtgcag gatttggcgg gcaaatggcg gagtggttgc
     3121 caccggcgca gagtgccgat gcggccctgc tacccgcgtt gcgtctgggg aatgcccggg
     3181 cagatgatct ggtgcgcaat aacggaatag cggctaatgc ggtggctctg cataaggatc
     3241 acattgtcgg gcatatgttt ctgatcagct accgtccgaa ctggcgctgg ctggggatgc
     3301 gggagaccgc agcaaaaagc tttgtcgatg aggtggaggc ggcctggtcg gaatacgccg
     3361 aagggatgtc tggcgagatc gacgtggaag gaaaacgcac gttcacggaa tttatccgtg
     3421 aaggtgtggg cgttcatgcg tttaacggcg aaatctttgt gcagccggtc tgggatacgg
     3481 aaaccacgca gttattccgt acgcgtttta aagccgtgag tccgaaacgg gtggacacgc
     3541 ctggacacgg tatggggaac cgttttctgc gggccggtgt ggaggtcgat cgatatggcc
     3601 gtgccgtcgc gtaccatatt tgtgaggatg attttccgtt ctctggtagt ggacgatggg
     3661 aacggatccc gcgtgaactt cccaccgggc gtccggctat gctgcatatt ttcgagccgg
     3721 tggaggacgg gcagacccgt ggggctaatc agttttacag cgtcatggaa cggctgaaga
     3781 tgctcgattc cctgcaggca acacagcttc agtcggccat agtgaaggcg atgtatgcag
     3841 cgacgattga aagtgaactt gataccgaaa aggcctttga atatatcgcc ggcgcgccac
     3901 aggagcagaa ggataatccg cttattaata ttctggagaa gttctccagc tggtatgaca
     3961 cgaatcacgt gacgctgggc ggtgtcaaaa ttccgcacct tttccctggt gatgatctga
     4021 aactgcagac agcgcaggat tcagacaatg gattttcggc gcttgaacag gcgctgctgc
     4081 ggtatatcgc cgccggtctt ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg
     4141 tcagttactc aagtgcccgc gcctccgcca atgagtcgtg gcgctatttt atggggcggc
     4201 gaaaatttat tgcggcccga ctggccacgc agatgttttc ctgctggctg gaagaggcac
     4261 ttcttcgggg gattattcgt ccgccacggg cacgttttga tttttatcag gcgcgatcag
     4321 cctggtcacg ggcagagtgg attggtgccg gaagaatggc cattgacggg ctcaaggaag
     4381 tccaggaatc agtgatgcgc attgaggccg gactgagcac gtatgagaaa gagctggcgc
     4441 tgatgggcga ggattatcag gacattttcc gccagcaggt cagggaatct gcagagcggg
     4501 aaaaagccgg actctcacgc ccggtgtgga tagcgcaggc gtatcagcag cagatagcgg
     4561 agagtcgcag gccggaagag gagacaacac cacgtgagac gtaatctttc acacattatt
     4621 gccgcagcat tcaatgaacc gctgcttctg gagcccgcct atgcgcgggt tttcttttgc
     4681 gcgctcgggc gcgagatggg ggcagcaagt ctttcggtac cacaacaaca ggtacagctt
     4741 gatgctcccg gaatgctggc tgaaacggac gagtacatgg ccggaggtaa acgaccggcc
     4801 cgtgtttacc gggtggtgaa cggtattgct gtactgccgg tgaccggcac gctggtgcac
     4861 cggctgggcg gtatgcggcc attttccgga atgacaggct atgacggcat tgtcgcctgt
     4921 cttcagcagg caatggcaga tagccaggtg cggggcgtac tgctggacat tgacagtccg
     4981 ggcgggcagg ccgccggcgc gtttgactgc gctgacatga tttaccgcct ccgtcagcag
     5041 aagccggtct gggcactgtg caatgacacg gcctgttctg cagccatgct gctggcgtcg
     5101 gcctgctccc gacggctggt tacccagaca tcccgtatcg gctccattgg cgtgatgatg
     5161 agccatgtca gctatgccgg tcatctggcg caggccgggg tggatatcac gctgatttat
     5221 gccggggcgc acaaggtgga tggcaatcag tttgaagcct taccggcaga ggtgcgtcag
     5281 gacatgcagc agcgggttga tgcggcgcac cggatgtttg ccgaaaaagt ggcgatgtat
     5341 acggggctgt ctgtggaagc tgtcacgggg acagaggctg ccgtttttga aggtcagtcc
     5401 gctattaagg ccggactggc ggatgaatta atcaatgcgt cggatgccat cagcgtgatg
     5461 gctgcggcgc tgaacactca tgatacagga ggcactatgc cgcaattaac tgcaacggaa
     5521 gctgccgcgc aggagaacca gcgagtgatg gggatcctga cgtgtcagga agcgaaagga
     5581 cgtgaacagc ttgccacgat gctggcagga caacagggca tgagcattga acaggcccgg
     5641 gctattctgg ccgcggcggc accgcagcag ccggtggcat ccgcgcagag tgaagccgat
     5701 cgcattatgg cgtgtgaaga agcgaaaggt cgtgaacaac tggcggcaac gctggcggcg
     5761 atgccggaga tgacggtgga aaaagcccgc ccgatcctgg ctgcctcacc gcaggcgaat
     5821 gccgggccct cacttcgtga tcagatcatg gccctggatg aggcaaaagg ggcagaagcg
     5881 caggctgaaa aactggcggc ctgcccggga atgaccgtgg agaacgcccg ggctgtgctg
     5941 gctgcgggat caggtaaggc cgaaccggtc tctgcatcca caaccgccct gtttgaacat
     6001 ttcatggcga atcattcacc ggcagcggtg cggggtggcg tgtcacagac gtcagcagac
     6061 ggtgatgcgg acgtgaaaat gctcatggcc atgccatgaa gtcagtgctg accatcaata
     6121 tgaggtttta acaaaatggt gacgaaaacc atcactgaac agcgtgcgga agtacgtatt
     6181 tttgctggta atgatccggc tcataccgcc acaggcagca gcgggatttc ttctgcaaca
     6241 ccggctctga cgcccctgat gctggatgaa gccaccggga aactggtggt ctgggatgga
     6301 cagaaagccg gtagtgcggt tggcatactg gtactgccgc ttgaaggcac agagacggtg
     6361 ctgacctatt acaagtcggg gacctttgcg acggaggcaa tccgctggcc tgacagtgtg
     6421 gatgaacaca aaaaggcaaa tgcctttgcc ggcacagccc tgagtcacgc ggctctgccg
     6481 taacacgtta tcaggccacc atggtggcct gactgatttc tgaatgaaag gaactgattt
     6541 atgggattgt ttacgacccg ccagttactc ggttataccg aacaaaaagt gaaatttcgt
     6601 gcgctgtttc tggagctgtt tttccgccgt acggtgaatt tccacaccga agaggtgatg
     6661 ctggacaaaa ttaccggaaa aacgccggtg gcggcctatg tctccccgat cgttgaagga
     6721 aaagtgctgc gccatcgtgg tggtgaaacc cgcgtgttgc gtccgggcta cgtcaagccg
     6781 aaacacgaat ttaattacca gcaggcggtt gagcgccttc ctggtgaaga tccggctcag
     6841 ctgaacgacc cggcctaccg tcgtctgcgt atcattaccg ataacctcaa acaggaagag
     6901 cacgccattg tccaggtgga agaaatgcag gcggtgaatg ccgtgctgta tggcaaatac
     6961 accatggaag gggatcagtt tgatactgtc gaggtggatt tcgggcgctc tgaaggaaat
     7021 aacattgagc aggctgacgg taaaaaatgg tctgagcagg accgtgatac gtttgatccg
     7081 acgcatgata ttgacctcta ctgcgatcag gccagcggcc ttgtgaatat cgccattatg
     7141 gacggtacgg tctggcgtct gctgaatggc tttaagctgt tccgcgaaaa actggatacc
     7201 cgtcgcggct caaattcaca actcgaaacg gcagtgaaag atctgggcgc agtggtgtcc
     7261 ttcaaggggt attacggcga tctggccatt gtggtggcga aaacgtctta tgtggcagag
     7321 gacggtaccg aaaaacgtta tctgccggag ggcatgctgg tgctggggaa tacggcggca
     7381 gaggggattc gttgctatgg tgccattaag gatgcacagg cgttgtctga aggagtggtg
     7441 gcttcttccc gttacccgaa acactggctg accgtgggcg atccgtcctg tgaattcacc
     7501 atgacgcagt ccgctccgct gatggtgctg ccggatccgg atgagtttgt ggtggtacag
     7561 gtgaaataat ccgtgagcgg gggcgaaatg cccccgtgtc ttttttcaca ggaggctgag
     7621 atggcaacaa aagaagaaaa tctgaatcgt cttcgtcaac tggctggcct gctggggcgc
     7681 gaggcggata tgtcggggag tgctgcggat attgctcaac gtgtgtctga gtgggaagag
     7741 gagcttgctg tttccccgga gggcattatg cactctgatg agagcggggc tgatcaaaat
     7801 cacacagacg atggtgagca gttgaacaac acggatgctc cggatgatgt taaagccgtc
     7861 cgggtacgga agtgcctgca agtaatgggg tattgcccgg agacaggtcg tcccgttgag
     7921 ctggcgttac ggggtatgcg tgttctggtg ccatcatcac tggcaacggc catgatacag
     7981 cacggaacgg ctgaatatgc gtgattttca gaatgccttt gatgctgccc tcgccggggt
     8041 agacagtacg atcgttgaag tgatggggct ctgtgcgcag ttcacctcgg gggcacagtg
     8101 tggcagcgaa gttcaggggg tttttgacga tccggagtcg ctggggtttg ccggtggcgg
     8161 ggtccgtatt gaaggaagca gcccgtcatt atttgtgcgg acggatacgg ttcgtgccgt
     8221 gcggcgtggt gacacgctga ccattaatgg tgagatattc tgggtggatc gtgtttctcc
     8281 ggatgacggg ggcagctgtt atctctggct caaccgtggt caaccacccg cagttaaccg
     8341 gcgacgataa acgcagggtg aaattatggc gataaaaggg cttgatcagg cgattgacaa
     8401 tctgagccgg gttcgtaaaa acgccattcc ggcggcttca gcaatggcca ttaaccgcgt
     8461 ggccacaacg gcgattaatc agtcttcatc acaggttgcc cgggagacaa aggttcgccg
     8521 gaaactggtt aaggaacggt ccagactgaa acgggcgacg gtcagaaatc cgaatgccag
     8581 aattatcgtt aaccgcggtg atctccctgt gattaagctg gggatcagga tgctggggcg
     8641 tcgcccgaac agcatactta aagccggtca gcatcggtat cagcgggcat ttattcagcg
     8701 attaaaaaat ggtcgctggc atgtcatgca gcgtgtggcc gggaaaaacc gttaccccat
     8761 tgatgtggtg aaaatcccga tggcggcccc actgaaacag gcatttgatg agaatgttga
     8821 ccgtatccgg cgtgaacgcc tgcctaaaga actggcatac gcgctgaaac aacaactgag
     8881 gattgcaata aaacgatgaa acacactgac attcgtgccg cagtgctgga tgcactcgag
     8941 cagcatgaac acggggcgac gctgtttgat ggtcgccccg ttgtttttga cgaagaggat
     9001 tttcctgcga tcgcggttta tctgacggat gcagagtata ccggtgaaga gctggatgca
     9061 gatacctggc gggccacgct gcatattgag gtgtttttac cggcacaggt accggattca
     9121 gagcttgatc agtggatgga aagccggatt tacccggcga tgaccgcgat cccggcactg
     9181 gcaggactga ttaccacgat ggttacgcag ggctatgagt atcgtcgtga tgacgatatg
     9241 gcgttatgga gttctgcaga tctgacttat tccattac
//
LOCUS       DAZUFB010000144         9225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZUFB010000144 DAZUFB010000000
VERSION     DAZUFB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.144"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            754..996
                     /locus_tag="ACHY3M_004409"
     CDS             754..996
                     /locus_tag="ACHY3M_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFS4319194.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1028..1678)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_004410"
     CDS             complement(1028..1678)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFS4319195.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1784..2983
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_004411"
     CDS             1784..2983
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFS4319196.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3015..3899)
                     /locus_tag="ACHY3M_004412"
     CDS             complement(3015..3899)
                     /locus_tag="ACHY3M_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS4319197.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4037..4429)
                     /locus_tag="ACHY3M_004413"
     CDS             complement(4037..4429)
                     /locus_tag="ACHY3M_004413"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4319198.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            4433..6184
                     /locus_tag="ACHY3M_004414"
                     /pseudo
     CDS             4433..6184
                     /locus_tag="ACHY3M_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            complement(6181..6690)
                     /locus_tag="ACHY3M_004415"
     CDS             complement(6181..6690)
                     /locus_tag="ACHY3M_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319199.1"
                     /translation="MSRIPFLRSYSASISAAFLSAFCASFCRFSACSINDLASRGDIR
                     RESASANSIRLSVCSRRASYPAISVRNPYSLSSACRSLNSCRVISAASAFPARSVFSV
                     FSSFSLAAIAAERRFPASVRLFSFSFCCALSVFLYAVISLSLFSISLRVGESSTHSGS
                     PQNSEKIVR"
     gene            6650..7924
                     /locus_tag="ACHY3M_004416"
     CDS             6650..7924
                     /locus_tag="ACHY3M_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFS4319200.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAINSARNNVSARTNEQKHANDALNALLK
                     EKENIRSQLADINQKIAEEKRKRDEINMVKDAIKLTSDFYRTIYDEFGKQASELAKEL
                     ASVSQGKQIKSVDDALNAFDKFRNNLNKKYSIQDRMAISKALEAINQVHMAENFKLFS
                     KAFGFTGKVIDRYDVAVELQKAVKTDNWRPFFVKLESLAAGRAASAVTAWTFSVMLGT
                     PVGILGFAIIMAAVSALVNDKFIEQVNKLIGI"
     gene            complement(7942..8289)
                     /locus_tag="ACHY3M_004417"
     CDS             complement(7942..8289)
                     /locus_tag="ACHY3M_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFS4319201.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDNQENARMFYAFIGC
                     IINAITFPFSMRIIQKMVIKFTGKELWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            8408..8755
                     /locus_tag="ACHY3M_004418"
     CDS             8408..8755
                     /locus_tag="ACHY3M_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFS4319202.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(8775..8960)
                     /locus_tag="ACHY3M_004419"
                     /pseudo
     CDS             complement(8775..8960)
                     /locus_tag="ACHY3M_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2078 a         2646 c         2643 g         1858 t
ORIGIN      
        1 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
       61 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
      121 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
      181 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
      241 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
      301 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
      361 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
      421 gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg
      481 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag
      541 cgtcggaaag gcgctggaag ccccgtagcg acgcggagag gggcgagaca agccaagggc
      601 gcaggctcga tgcgcagcac gacatagccg gttctcgcaa ggacgagaat ttccctgcgg
      661 tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat tcgcgagagc cttgagtcca
      721 cgctagatct atctcatctg cgcaaggcag aacgtgaaga cggccgccct ggacctcgcc
      781 cgcgagcgcc aggcgcacga ggccggcgcg cggacccgcg ccacggccca cgagcggacg
      841 ccgcagcagg agcgccagaa ggccgccaga gaggccgagc gcggccgtga ggcttggacg
      901 ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa
      961 gggggagggg atgttgtcta catggctctg ctgtagtgag tgggttgcgc tccggcagcg
     1021 gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt
     1081 tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt
     1141 cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc
     1201 cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga
     1261 agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga
     1321 ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat
     1381 ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga
     1441 tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca
     1501 gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg
     1561 ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga
     1621 cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc
     1681 ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc
     1741 gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacagacc
     1801 cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc
     1861 ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg
     1921 cattctgctg gcgctgtatg cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct
     1981 gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga
     2041 ctacgccatc atggcgacgg cgcctttcct ttgggttctc tatatcgggc ggatcgtggc
     2101 cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata tcactgatgg
     2161 cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc
     2221 gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt tcttcgccgc
     2281 ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg agtcgcacaa
     2341 aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc
     2401 ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg
     2461 acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact gggacgcgac
     2521 cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc aggcaatgat
     2581 caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg gaatgattgc
     2641 cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat
     2701 catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag
     2761 gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc tcaccagcct
     2821 gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta taacaacgtg
     2881 gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc cggcgctgcg
     2941 tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga aacgataggc
     3001 ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctagg agtgcggttg
     3061 gaacgttggc ccagccagat actcccgatc acgagcagga cgccgatgat ttgaagcgca
     3121 ctcagcgtct gatccaagaa caaccatcct agcaacacgg cggtccccgg gctgagaaag
     3181 cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca aaggaagtag
     3241 gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt tcctgtaggc
     3301 atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc tccggccgcc
     3361 agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg ggtcagcacg
     3421 gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac aggatctagc
     3481 gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat agcccccagg
     3541 accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac gaccatcagc
     3601 ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac cgaaataaac
     3661 aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg catccaccag
     3721 attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg caacgcccgc
     3781 agcatcgcga ccgtcatcgg tgagaagttc ggcaggtatt gggtggtgac aatgtaggtg
     3841 ctgccccaaa tggcaggtgc tatcgctgtg aacaataaat cgggcgtgcg taatgacatg
     3901 cgattacaag acctccgcta cggcgatggg tttttgagag ttgcaggcgc cgcgatgacc
     3961 gcaccctgac ttggactggc cctgcctcgc accaatccac ctgcggctat ggccgcgatc
     4021 atgcgaggcc cgaaaattac acgacctgta cacgtccgcc gaacccggaa cgcaacagag
     4081 cttcgtgggc atcccgatcc gggtcgtagc agcagtcctg gaccaaacgc acgtcgtagt
     4141 ccgcatcact agcccaggcg acgcttgaaa gaacaacgcc ggtggtgctt atcccggcca
     4201 tgacaagcgt gcttacgccg cgcgtccgaa ggtcggcgtc gagcgccgtg ccataaaaga
     4261 cgctggctcg cggacatgca tagaaaaggt cgccccgttc gatcgcaagc ccttcgacgg
     4321 gcagaccggt gcgaaatcgt ccgctcggga gatacggtga gatttggcga ttcgtcgccg
     4381 gcggcgcatg ttcatactct tcgccgagcg aaaagttggg gaacagcacg ggccggccgc
     4441 gcaaggcgtg ctggcagccg tgcagaccct gcgtgagatg aacgccgaca acctgcgcaa
     4501 ggtgccggcc gatgcaccca cggccttcat caagccgcgc tggaagccgc tggtgatcac
     4561 cccggaaggc ctcgaccgga aattctacga aatctgcgcc ctgtccgagc tgaagaacgc
     4621 cctgcgctcc ggcgacatct gggtcaaggg ctcgcggcag ttccgcgact tcgacgacta
     4681 cctgctgccg gccgagaagt tcgccgcact caagcgcgag caggccctgc ccctggcgat
     4741 caacccgaac agcgaccagt acctggaaga gcgtttgcag ctgctggacg agcagttggc
     4801 caccgtcacc cgcctggcca aggacaacga gctgcccgat gccatcctca ccgagtcagg
     4861 gctgaaaatc accccgctgg atgcggcggt gccggatcgg gcgcaggcgc tgatcgacca
     4921 aaccagccag ttactgccgc gcatcaagat caccgaactg ctgatggacg tggacgactg
     4981 gacgggcttc agccgccact tcacccactt gaaggacggg gccgaggcca aagacaggac
     5041 gttgctgctg tccgcaatcc tcggtgatgc gatcaacctc gggctgacca agatggccga
     5101 gtcgagcccc ggcctgacct acgccaagct gtcctggctg caagcctggc acatccgcga
     5161 cgaaacctat tcggcggcct tggccgagct ggtcaaccac cagtatcgcc acgcctttgc
     5221 cgcccactgg ggcgacggca cgacctcatc ctccgatggc cagcgcttcc gcgcgggtgg
     5281 ccggggcgag agcaccgggc acgtcaaccc gaagtacggt agcgagccgg gacggctgtt
     5341 ctatacccat atctccgacc agtacgcgcc gttcagcacc cgcgtggtga atgtcggcgt
     5401 ccgcgattcc acctatgtgc tcgacggcct gctgtaccac gagtccgacc tgcggatcga
     5461 ggagcactac accgacacgg ccggcttcac cgatcacgtc tttgccctga tgcacctgct
     5521 aggcttccgc ttcgcgccgc gcatccgcga cctcggcgaa accaagctgt acgtgccgca
     5581 gggcgtgcaa gcctacccga cgttgcgccc gctgatcggc ggcaccctga acatcaagca
     5641 cgtgcgtgcc cactgggacg acatcctgcg cctggccagc tcgatcaagc agggcaccgt
     5701 caccgcctcg ctgatgctgc gcaagctcgg cagctacccg cgccagaacg gactggccgt
     5761 ggccctgcgc gagctgggcc ggatcgagcg cacgctgttc atcctggact ggctgcaaag
     5821 tgttgaactg cgccgccgcg tgcatgccgg cctgaacaaa ggtgaggcgc gcaactcgct
     5881 ggccagggcg gtgttcttca accgccttgg ggaaatcagg gatcggagct tcgagcagca
     5941 gcgctaccgg gccagcggcc tcaacctggt gacggcggct atcgtgctgt ggaacacggt
     6001 gtacctggaa cgcgccaccc aggggttggt cgaggccggc aagccggtgg acggcgagct
     6061 gctgcaattc ctgtcgccgc tgggctggga gcacatcaac ctaaccggcg attacgtctg
     6121 gcggcagagc cgcagactgg aagacgggaa gtttcggccc ttacggatgc ccggaaaacc
     6181 ttagcgtacg attttttccg aattctgcgg gctccccgag tgggtcgatg attccccaac
     6241 ccgaagtgat atcgaaaaaa gggacaagga aatcacagcg tacaaaaaca cgctcagcgc
     6301 gcagcagaaa gaaaatgaga ataagcgcac tgaagccgga aaacgcctct ctgcggcgat
     6361 tgctgcaagg gaaaaagatg aaaacacact gaaaacactc cgtgccggaa acgcagatgc
     6421 cgctgatatt acacgacagg agttcagact cctgcaggca gagctgagag aatacggatt
     6481 ccgtactgaa atcgccggat atgacgccct ccggctgcat acagagagcc ggatgctgtt
     6541 tgctgatgct gattctcttc gtatatctcc ccgggaggcc aggtcgttaa tcgaacaggc
     6601 tgaaaaacgg cagaaggatg cgcagaacgc agacaagaag gccgctgata tgcttgctga
     6661 atacgagcgc agaaaaggta ttctggacac gcggttgtca gagctggaaa aaaatggcgg
     6721 ggcagccctt gccgttcttg atgcacaaca ggcccgtctg ctcgggcagc agacacggaa
     6781 tgacagggcc atttcagagg cccggaataa gctcagttcg gtgacggaat cgcttaacac
     6841 ggcccgtaat gcattaacca gagctgaaca acagctgacg caacagaaaa acacacctga
     6901 cggcaaaacg atagtttccc ctgaaaaatt cccggggcgt tcatcaacaa atcattctat
     6961 tgttgtgagc ggtgatccga gatttgccgg tacgataaaa atcacaacca gcgcagtcat
     7021 cgataaccgt gcaaacctga attatcttct gacccattcc ggtctggact ataaacgcaa
     7081 tattctgaat gaccggaatc cggtggttac agaggatgtg gaaggtgaca agaaaattta
     7141 taatgctgaa gttgctgaat gggataagtt acggcaacga ttgcttgatg ccagaaataa
     7201 aatcacctct gctgaatctg cgataaattc ggcgagaaat aacgtcagtg ccagaacaaa
     7261 tgaacaaaag catgcaaatg acgctcttaa tgccctgttg aaggaaaaag agaatatccg
     7321 tagccagctt gctgacatca atcagaaaat agcggaagag aaaagaaaaa gggatgaaat
     7381 aaatatggta aaggatgcca taaaactcac ctctgatttc tacagaacga tatatgatga
     7441 gttcggtaaa caagcatccg aacttgctaa ggagctggct tctgtatctc aagggaaaca
     7501 gattaagagt gtggatgatg cactgaacgc ttttgataaa ttccgtaata atctgaacaa
     7561 gaaatatagc atacaagatc gcatggccat ttctaaagcc ctggaagcta ttaatcaggt
     7621 ccatatggcg gagaatttta agctgttcag taaggcattt ggttttaccg gaaaagttat
     7681 tgatcgttat gatgttgctg tggagttaca aaaggctgta aaaacggaca actggcgtcc
     7741 attttttgta aaacttgaat cactggcagc aggaagagct gcttcagcag ttacagcatg
     7801 gacgttttcc gtcatgctgg gaacccctgt aggtattctg ggttttgcaa ttattatggc
     7861 ggctgtgagt gcgcttgtta atgataagtt tattgagcag gtcaataaac ttattggtat
     7921 ctgacacata atgatgccct ttcattttcc tgaaagggct ttgcagaata taaaaattaa
     7981 gtaaatggca accacaggaa ctgatataac gaaacagaat aattcaaata ttgcagttaa
     8041 gcttccgcca actggatttg taaagaagtc tttttgccag agttctttcc ctgtaaactt
     8101 tattaccatt ttctgtatta tcctcattga aaaaggaaaa gtaatggcat tgataatgca
     8161 tccgatgaac gcataaaaca ttcttgcatt ttcctggtta tctttccatc ccagataaaa
     8221 aactgtaaga ataatgaggc ttggtatcag gcttttcagt aaatacttta cagatatatc
     8281 cagtttcatc tggtttctcc tttattgtta tcgtgtaccg gtgcgtgata acataatcac
     8341 agcatgataa taataatcaa taacaataag ctgtgtcacg tttacatcat ggaacggagg
     8401 tgctcctgtg acctttgagc agaaaaaagc ccgggccatt gccctgatgg acagcaaaaa
     8461 gatgtggcgg agcaattatg cccctcctct tctgcgtatt ctatggcggc tgggtatccg
     8521 gcttccgcca ttacccttta tgccgttctg gcaggtcacg gtgctgatgg ggggcttatg
     8581 gggcatttcc tggggatgtg caatgtggtt tatctactgg gggccgtcag gaatggtggc
     8641 aggtgaggcc attattataa gtatcacggg tggttttttg ttcggactgt gcatggcctc
     8701 tttccactgg tggcgcagga aagttaaccg gctgccacca tgggatgatg tgtaaaccgt
     8761 gcacaggcgt gttcttacgg agcggcactt tcatttatct gtacgcgaac ttctattcct
     8821 gcgtcatgac aggccagcag ccactgcgcc acttccagcg gatcgccctc ccggcgtacc
     8881 accctgcctt ctttattcca taactgcaga caggtgctgc cgtcgagacg caccacaaaa
     8941 tccccacggc aggcctgata ggggtccgct tggaattcgg aaaataaacc acgttaagac
     9001 tattttttag tcaaaggact ttcaggaatc gccagaatgt cgggacaaca gcggttttgg
     9061 tgtccgaaat cgtacaattc tgtacatcta cgcaagggaa tgtacggaag taagccaaac
     9121 ggttatcgga cggccggaac atttcgtaca gcctttacgt tcagtgagtt agctggcctg
     9181 gatgtaacca ctcgttcaac tcaattctct tagcgtggga aaatt
//
LOCUS       DAZUFB010000145         9148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZUFB010000145 DAZUFB010000000
VERSION     DAZUFB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.145"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..124
                     /locus_tag="ACHY3M_004420"
     CDS             <1..124
                     /locus_tag="ACHY3M_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4319203.1"
                     /translation="NGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
     gene            complement(363..3725)
                     /gene="mscK"
                     /locus_tag="ACHY3M_004421"
     CDS             complement(363..3725)
                     /gene="mscK"
                     /locus_tag="ACHY3M_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFS4319204.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMCNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3853..4500)
                     /gene="acrR"
                     /locus_tag="ACHY3M_004422"
     CDS             complement(3853..4500)
                     /gene="acrR"
                     /locus_tag="ACHY3M_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFS4319205.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4642..5835
                     /gene="acrA"
                     /locus_tag="ACHY3M_004423"
     CDS             4642..5835
                     /gene="acrA"
                     /locus_tag="ACHY3M_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFS4319206.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5858..9007
                     /gene="acrB"
                     /locus_tag="ACHY3M_004424"
     CDS             5858..9007
                     /gene="acrB"
                     /locus_tag="ACHY3M_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFS4319207.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2212 a         2339 c         2405 g         2192 t
ORIGIN      
        1 taatggcaag aaaggaaagg tgattctcgg cgcgatgatg cgcaagctgg cacaggtggc
       61 gtatggagtg ctgaagtcag gcgtgccgtt cgatgcgtca cggcataatc cggtagcggc
      121 gtaaaaatcg cggaagggat gaaaaaaaca gcgcctgacg gcgctgtgtc tggcatgcct
      181 gcaatccggg aaaccggacc aggaaaaaac ttgcaggcca taacagtatc tacaccgctc
      241 ctgcaataca ttgaattctt gcggtttcgt aggccggata aggcggtcac gccgcatccg
      301 gcatgaacaa cgcgcacttt gtcagtaatc tcggggcaac taaatgtcgc cccctttggt
      361 tattacccta ccgctggcgt cgggtcatcg cctttgtagt cgcgtttgac ttccgtcacc
      421 tcatcgccct tctcgttgtg cagatgcact tcaagctggt taaaggcaat gttgatgtcg
      481 ttttcacggc acagctgatc gatagtacgg ttcagctcat cgacagtacg actacggtca
      541 cgcagttcac gcacatacag acgcagctca tgatccaacg tgctggcacc aaacgccgta
      601 aagaagactt ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc cgccttcagt
      661 aacactttac gcactttttc cagatcggag ccataggcca cgccgagacg gatcaccaga
      721 cgcgtagtag tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa cgctttgttc
      781 gggatgatca cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat acggatctta
      841 cttaccgtcc ccgagaagct accaatggtt accgtatcgc caatacgcac cggacgttcg
      901 aacagaatga tcaaaccgga gacgaagtta ccgaaaattt cttgtaaacc aaaaccaaga
      961 cctacggata atgctgcggc cagccactgg agtttatccc aagagacgcc cagcgatccg
     1021 aacaccgtca tcgcaccaac ggcaataatg atgtagttaa ggatggtagt aatggcatac
     1081 gacgcgccct gacgcatatt cagtcgcgag agcaccagca cttccagtaa accaggcagg
     1141 ttgcgaatca acgcccaggc caccattgag gcgataatcg caaacaacag actgcccatg
     1201 gtgacgtttt tcaccaccgc agcgccagct tcagtgccgt tgtaatgcca gagcgtgatg
     1261 ctgtcgagat agctgaacac ggtgatcaaa tcggaccaaa ttgcccagaa catgacaccg
     1321 aacagcgcaa acatcagcaa catggtaata cgcagcgtct gctggttaac ctgttccagt
     1381 gcaatggtgg gttcttccgg cggttcggca ccttctgcgc cctctttcac cagattctgc
     1441 cgacgcgcca gcgcacgacg ccaggcgata cgccgcgccg ctacgcttaa gccgcgcaat
     1501 accgtctggt agagcaagtt ccagatgatc accagataaa cggtttcaat ccaacgcccg
     1561 gagaggcgca gcgtggtgta gaaataaccc gtggcagtca gcaccatcaa tgcaatcggg
     1621 ataatcgaca gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt atcacgccag
     1681 ctttcgcggc acattggcca taccaggaag gcaatcagca gcaggttgaa gaaaatcatc
     1741 gcctgcccca gcacatcatc catcagatgc agcggggaaa gttctgccac cacagaccag
     1801 aaatggatag gcagcaacgc gagactgatg cggacaattt gccgacgcca gtggctggtc
     1861 tgctgctccg gcatgccgaa gtgacgcacg gcaacgccat ttttctccag taccttccag
     1921 cacaggccaa acaccagcca gaaaatcgcc agttttttac tgaacgacca tagcagttcg
     1981 ctgatgttga gctgcatggt caacagaatc aggccaaccg cgagaataat caggcacacc
     2041 ggcagcgcgc ggatcaggtc gataaggatc gcttttggtg tgttgagctg gctatcgtta
     2101 cgcagggaac ccacagcgga agccagtttc tgttgatacg ctttcagcca gcccagacgc
     2161 cagtggatca gcccggcaat caacagcagc ggcaaaccgg cgaggaaagc gataaaaacg
     2221 gcaggccagg ctttttccca gttcaccgtg attttcatcg acttaaattc atctttcagg
     2281 ctttgcggga acgctttgat ccagtcccag tccattggac ggttactgtt cacccaaaag
     2341 atttgctgag tcaggatgga tttcaggttt ttcgatacgc tcattaactg ctgctggttg
     2401 atttgcaggt taatggccat catcagctgg ttacccaact gtttgttgag ttgatccagc
     2461 aattcgcgac gcatatcaac cacttgcaat aacgcatcgt gaacttcgct gttgacttcg
     2521 ttggtgtgac cttcttccag tttgttgacg aacgcatcgc tctggaagag tgcatcacgc
     2581 tgctggttaa cttcaaactg ttcgagacgc aaatccgcga tgcggttggt catgttttcc
     2641 agttcatccg ccgagggcag cgtttgttgt tgctggtaaa ggatacgaga caacagcagg
     2701 ctgcccttca ggacggcaat ctgctcttta atattgcgtt ccgattgcag cgcccgctcc
     2761 agccagtttt tgactttaat gttttgctgc atcaactgat taccgttttc agtcgcggta
     2821 atcagacgct gacttaactg ctggttaatt tccagttcct gtttcaccag cggattagcc
     2881 tgaatacgcg cggcttcatc cggggagacg gcttcctgcg ccgttttttc agttaaagtc
     2941 aggcgcttgc tgtttaccgc ttcttgcaac agctgcaact ggtgctccag acgggcgctg
     3001 ttcgccgtca cgtaatcacg ttgcttttgc aaggtatcct gcaagacggt gttcccttcc
     3061 aggcttttac gctgctggtc aatctccgca ttcagcaacg cctgctgagc ctgcattaac
     3121 actttctggc tgggacgtaa ggcagtctcg ccgacatcag tcccatccag acgactgcga
     3181 atttgttgca gctgctgcga agcgttacac atcgcatttt gcacgcgttc gggctgcgtc
     3241 tgtaacgaaa ccagctggct gttataagac gccagatcgt tttgtgcgtt ttgcaaatcg
     3301 tccagcgcct gggcaacgcg agtttccagc tggcgcaacg aaagcgtact cagaattttg
     3361 cgcgtttctt cgtcgttatc gacatcgcta agtgctgtta acgccgcggt cgcctggcgc
     3421 attttttccg gcgcttcagc gactttttgc cgtagctgaa ctgtctcttc ttttatgcga
     3481 tcgattttat cgagggtggc taatgtatct gtcagatcct gctgcaccag tttgtcctga
     3541 gcagaaagat ctttttgttt atttagtgag tcaagttgcg cctgcaggtc cgcttttgtc
     3601 ggcagatcac cattcgatga cgcccgcgca aacgccgtgt tctggcataa caagacaaaa
     3661 acaaaagcaa taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata ctggaacata
     3721 gtcatgatga atgaaggttt ctgaacctga agaacgacct gaaaaagtca aaccgcaaga
     3781 atatcacgac gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa aatcctggag
     3841 tcagattcag ggttattcgt tagtggcagg attacgaagc gtggggcaca ggagatacat
     3901 ctccagtaag atggcaacgt aatcgcgggc ttctttttta agatcaaaag attgcggggc
     3961 aaagagccag ttttccatca ggccggaaat atagccgcgc ataataattg ctgcgcgacg
     4021 cgtcattaaa tccgcaggca acattttcgc ttcaatacaa tgttttaacg tttgttctat
     4081 acggtcataa ctttccagac agagattacg ttgtgcctgt tgcacaacag ccatttctcc
     4141 gacaaattcg catttgtgga atataatctc catcaataat cgacgccgtt cttctgtcac
     4201 cgtggattca agaacatgaa ttaatatctc tcttaatact gagagtggat cgccagggaa
     4261 ttttgcctga tactcaagct ctagttcacc aatattggat tctgacagtt cccagatctc
     4321 actgaacaaa tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg taacgccagc
     4381 tgcttttgca atctcaccca gcgaggtgga tgataccccc tgctgtgaga aaagacgtag
     4441 agccacatcg aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt ttcgtgccat
     4501 atgttcgtga atttacaggc gttagattta catacatttg tgaatgtatg taccatagca
     4561 cgacgataat ataaacgcag caatgggttt attaactttt gaccattgac caatttgaaa
     4621 tcggacactc gaggtttaca tatgaacaaa aacagagggt ttacgcctct ggcggtcgtt
     4681 ctgatgctct caggcagctt agccctaaca ggatgtgacg acaaacaggc ccaacaaggt
     4741 ggccagcaga tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct gcagatcaca
     4801 accgagcttc cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc tcaagttagc
     4861 gggattatcc tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagctgg tgtctctctc
     4921 tatcagattg atcctgcgac ctatcaggcg acatacgaca gtgcgaaagg tgatctggcg
     4981 aaagcccagg ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca gaaactgctc
     5041 ggtactcagt acatcagtaa gcaagagtac gatcaggctc tggctgatgc gcaacaggcg
     5101 aatgctgcgg taactgcggc gaaagctgcc gttgaaactg cgcggatcaa tctggcttac
     5161 accaaagtca cctctccgat tagcggtcgc attggtaagt cgaacgtgac ggaaggcgca
     5221 ttggtacaga acggtcaggc gactgcgctg gcaaccgtgc agcaacttga tccgatctac
     5281 gttgatgtga cccagtccag caacgacttc ctgcgcctga aacaggaact ggcgaatggc
     5341 acgctgaaac aagagaacgg caaagccaaa gtgtcgctga tcaccagtga cggcattaag
     5401 ttcccgcagg acggtacgct ggaattctct gacgttaccg ttgatcagac cactgggtct
     5461 atcaccctac gcgctatctt cccgaacccg gatcacactc tgctgccggg tatgttcgtg
     5521 cgcgcacgtc tggaagaagg gcttaatcca aacgctattt tagtcccgca acagggcgta
     5581 acccgtacgc cgcgtggtga tgccaccgta ctggtagttg gcgcggatga caaagtggaa
     5641 acccgtccga tcgttgcaag ccaggctatt ggcgataagt ggctggtgac agaaggtctg
     5701 aaagcaggcg atcgcgtagt aataagtggg ctgcagaaag tgcgtcctgg tgtccaggta
     5761 aaagcacaag aagttaccgc tgataataac cagcaagccg caagcggtgc tcagcctgaa
     5821 cagtccaagt cttaacttaa acaggagccg ttaagacatg cctaatttct ttatcgatcg
     5881 cccgattttt gcgtgggtga tcgccattat catcatgttg gcaggggggc tggcgatcct
     5941 caaactgccg gtggcgcaat atcctacgat tgcaccgccg gcagtaacga tctccgcctc
     6001 ctaccccggc gctgatgcga aaacagtgca ggacacggtg acacaggtta tcgaacagaa
     6061 tatgaacggt atcgataacc tgatgtacat gtcctctaac agtgactcca cgggtaccgt
     6121 gcagatcacc ctgacctttg agtctggtac tgatgcggat atcgcgcagg ttcaggtgca
     6181 gaacaaactg cagctggcga tgccgttgct gccgcaagaa gttcagcagc aaggggtgag
     6241 cgttgagaaa tcatccagca gcttcctgat ggttgtcggc gttatcaaca ccgatggcac
     6301 catgacgcag gaggatatct ctgactacgt agcggcgaat atgaaagatg ccatcagccg
     6361 tacgtcgggc gtgggtgatg ttcagttgtt cggttcacag tacgcgatgc gtatctggat
     6421 gaacccgaat gagctgaaca aattccagct aacgccggtt gatgtcatta ccgccatcaa
     6481 agcgcagaac gcccaggttg cggcgggtca gctcggtggt acgccgccgg tgaaaggcca
     6541 acagcttaac gcctctatta ttgctcagac gcgtctgacc tctactgaag agttcggcaa
     6601 aatcctgctg aaagtgaatc aggatggttc ccgcgtactg ctgcgtgatg tggcgaagat
     6661 tgagctgggt ggtgagaact acgacatcat cgcagagttt aacggccaac cggcttccgg
     6721 tctggggatc aagctggcga ccggtgcaaa cgcgctggat accgctgcgg caatccgtgc
     6781 tgaactggcg aagatggaac cgttcttccc gtcgggtctg aaaattgttt acccgtacga
     6841 caccacgccg ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg tcgaagcgat
     6901 catcctcgtg ttcctggtta tgtatctgtt cctgcagaac ttccgcgcga cgttgattcc
     6961 gaccattgcc gtaccggtgg tattgctcgg gacctttgcc gtccttgccg cctttggctt
     7021 ctcgataaac acgctaacaa tgttcgggat ggtgctcgcc atcggcttgt tggtggatga
     7081 cgctatcgtt gtggtagaaa acgtggagcg tgttatggcg gaagaaggtt tgccgccaaa
     7141 agaagctacc cgtaagtcga tggggcagat tcagggcgct ctggtcggta tcgcgatggt
     7201 actgtcggcg gtattcgtac cgatggcctt ctttggcggt tctactggtg ctatctatcg
     7261 tcagttctct attaccattg tttcagcaat ggcgctgtcg gtactggtag cgttgatcct
     7321 gactccggct ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc acggggaagg
     7381 taaaaaaggc ttcttcggct ggtttaaccg catgttcgag aagagcacgc accactacac
     7441 cgacagcgta ggcggtattc tgcgcagtac ggggcgttac ctggtgctgt atctgatcat
     7501 cgtggtcggc atggcctatc tgttcgtgcg tctgccaagc tccttcttgc cagatgagga
     7561 ccagggcgta tttatgacca tggttcagct gccagcaggt gcaacgcagg aacgtacgca
     7621 gaaagtgctc aatgaggtaa cgcattacta tctgaccaaa gaaaagaaca acgttgagtc
     7681 ggtgttcgcc gttaacggct tcggctttgc gggacgtggt cagaataccg gtattgcgtt
     7741 cgtttccttg aaggactggg ccgatcgtcc gggcgaagaa aacaaagttg aagcgattac
     7801 catgcgtgca acacgcgctt tctcgcaaat caaagatgcg atggttttcg cctttaacct
     7861 gcccgcaatc gtggaactgg gtactgcaac cggctttgac tttgagctga ttgaccaggc
     7921 tggccttggt cacgaaaaac tgactcaggc gcgtaaccag ttgcttgcag aagcagcgaa
     7981 gcaccctgat atgttgacca gcgtacgtcc aaacggtctg gaagataccc cgcagtttaa
     8041 gattgatatc gaccgggaaa aagcgcaggc gctgggtgtt tctatcaatg acattaacac
     8101 cactctgggc gctgcatggg gcggtagcta tgtgaacgac tttatcgacc gcggtcgtgt
     8161 gaagaaagtt tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg atatcggcga
     8221 ctggtatgtt cgtgctgctg atggtcagat ggtgccgttc tcggcattct cctcttctcg
     8281 ttgggagtac ggttcgccgc gtctggaacg ttacaatggc ctgccatcca tggaaatctt
     8341 aggccaggcg gcaccgggta aaagtaccgg tgaagcaatg gagctgatgg aacaactggc
     8401 gagcaaactg cctaccggtg ttggctatga ctggacgggg atgtcctatc aggaacgcct
     8461 ctccggcaac caggcacctt cactgtacgc gatttcgttg attgtcgtat tcctgtgtct
     8521 ggcggcgctg tacgagagct ggtcgattcc gttctccgtt atgctggtcg ttccgctggg
     8581 ggttatcggt gcgttgctgg ctgccacctt ccgtggcctg accaatgacg tttacttcca
     8641 ggtaggcctg ctcacaacca ttgggttgtc ggcgaagaac gcgatactta tcgtcgaatt
     8701 cgccaaagac ttgatggata aagaaggtaa aggtctgatt gaagcgacgc ttgatgcagt
     8761 gcggatgcgt ttacgtccga tcctgatgac ctcgctggcg tttatcctcg gcgttatgcc
     8821 gctggttatc agtactggtg ctggttccgg cgcgcagaac gcagtaggta ccggtgtaat
     8881 gggcgggatg gtgaccgcaa cggtactggc aatcttcttc gttccggtat tctttgtggt
     8941 ggttcgccgc cgctttagcc gcaagaatga agatatcgag cacagccata ctgtcgatca
     9001 tcattgatac aacgtgtaat cactaaggcc gcgcaagcgg ccttttttat gcataaccta
     9061 cgaacattaa ggagtaattg aaccaccaac tcaggatctc atacgaaaac cagtattaac
     9121 cacggataaa attcataaaa aatactga
//
LOCUS       DAZUFB010000146         8915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZUFB010000146 DAZUFB010000000
VERSION     DAZUFB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.146"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..572
                     /locus_tag="ACHY3M_004425"
                     /pseudo
     CDS             1..572
                     /locus_tag="ACHY3M_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1444..1584)
                     /locus_tag="ACHY3M_004426"
     CDS             complement(1444..1584)
                     /locus_tag="ACHY3M_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4319208.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1886..2149)
                     /locus_tag="ACHY3M_004427"
     CDS             complement(1886..2149)
                     /locus_tag="ACHY3M_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4319209.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2574..2699
                     /locus_tag="ACHY3M_004428"
                     /pseudo
     CDS             2574..2699
                     /locus_tag="ACHY3M_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            2764..3024
                     /locus_tag="ACHY3M_004429"
                     /pseudo
     CDS             2764..3024
                     /locus_tag="ACHY3M_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3074..3313
                     /locus_tag="ACHY3M_004430"
                     /pseudo
     CDS             3074..3313
                     /locus_tag="ACHY3M_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3361..3978)
                     /gene="ureG"
                     /locus_tag="ACHY3M_004431"
     CDS             complement(3361..3978)
                     /gene="ureG"
                     /locus_tag="ACHY3M_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFS4319210.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3990..4664)
                     /locus_tag="ACHY3M_004432"
     CDS             complement(3990..4664)
                     /locus_tag="ACHY3M_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFS4319211.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLVRLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(4665..5129)
                     /gene="ureE"
                     /locus_tag="ACHY3M_004433"
     CDS             complement(4665..5129)
                     /gene="ureE"
                     /locus_tag="ACHY3M_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFS4319212.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(5139..6842)
                     /gene="ureC"
                     /locus_tag="ACHY3M_004434"
     CDS             complement(5139..6842)
                     /gene="ureC"
                     /locus_tag="ACHY3M_004434"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFS4319213.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(6835..7155)
                     /locus_tag="ACHY3M_004435"
     CDS             complement(6835..7155)
                     /locus_tag="ACHY3M_004435"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFS4319214.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(7164..7466)
                     /locus_tag="ACHY3M_004436"
     CDS             complement(7164..7466)
                     /locus_tag="ACHY3M_004436"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFS4319215.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(7557..8255)
                     /locus_tag="ACHY3M_004437"
     CDS             complement(7557..8255)
                     /locus_tag="ACHY3M_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFS4319216.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            8636..8911
                     /locus_tag="ACHY3M_004438"
     CDS             8636..8911
                     /locus_tag="ACHY3M_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319217.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         2278 a         2214 c         2145 g         2278 t
ORIGIN      
        1 agacatagcg ttatatatat tggcattttt atcagccgga tatacattcc agtatattta
       61 caatgacaat atattttcag aactatgggg agttggcctg tttgttactc tgatagtact
      121 gaatatagta tttaatctct tttttagact cttattcatt ctctcgaaaa tgatattaat
      181 gtcacgacat ataagaaaga tgcaaaaaaa actaaaacag acgtctgtgc ctgattttat
      241 ttattattca atagaaaaac acaacaaata tcccatatat gtattacaca tgccatcaaa
      301 caatataatt gaaataggct acaatataat aaacaccgat ctggtcattg gcgaaaaaat
      361 tcatttcagg acactaagca atcgttcttt atattttaac ctcacccaac ccccattaat
      421 cgccacaata aaagatgatg tgttttgcac tcttcatgat tactataacc acaataatga
      481 aacgaaatcc actattgaca attacatatc aaaaataaag gataatcata atacccccat
      541 ggttattaaa caacgaaaat gttcaggaat aaaaaaaaag agacaattcc cgagataaaa
      601 caaaagagca taaacatatg aaccggaaat tctatttcaa aaatatcctg tggggaatgt
      661 gccccattat aatatccata ttgcatctga tatatgacac accaacagaa gcatcgataa
      721 aagttgactt atttctgata ctcagtggcc tggcccttcc ttgttcaata tacctgacaa
      781 aaacaatcac actaaaatac acatccatat cattctggaa aaaacctctt ttctggtctt
      841 atggtatata tcattttcat cttgtttata taatgatatg tatagtttta gcaatacctt
      901 ctgcaattat cgttttattc tttcactttt atcaaaacat caccagaaac tggaagtgac
      961 aaccgcaata atgaacataa aaaggcaaat cagaatggca gtaaatcaat aaagtaccat
     1021 ttcatatgaa tcatcagaaa aacggtggac gcgacatcca gcacgaggca aaacacagca
     1081 acaaaataag ccggatatcg cgccctgtac ttttctaact caactcccgg cattgtgcaa
     1141 gttttaatcg caggtggatt cagagccacg cccccattga ttcagcctga agacacgtat
     1201 atccttggag aaagcaggaa tctcattgct gtgaaattcc ttacagaatc ttcagcccca
     1261 ttttatgggg ctttttttgg tgcccccacg ctgttaccac actttcggga tgcgaataaa
     1321 aaagcccaca gtttttacac tgcggacttt tgttttttct ttgtgatttg tgtgcaaggg
     1381 tgcgactttc gaccgaacct ttggcaaccg gatagcgggg actgttgtcc ccgctgcggt
     1441 ttcttacggt ttacactgca agaacgccgc aatttccgac cacatgaagc ttaccctgaa
     1501 ttcacacggt gatgcgtgta acacccagac ggtcagcaac atggtcaggc ataacatgcc
     1561 cacaatgatc gttttttgcg gcatagcact tgctcctttt acagagaggc gctaaccttc
     1621 cacttgctga aggtagatgg tcagggcctc gggttaacgt ttaagttgac tcggggcctt
     1681 tccacatccg gccttcaggt aatccttcca gccgtcagcc gaaaggcacc cgcgcgtaat
     1741 gtactggttt tcagagaggt ccgcaatctt ccggtgacga gatcatctgc acacgccccg
     1801 gtgggacggg ctaacggtat attcaggtgc aggatctctc tgtctggtgc gtttttaaat
     1861 tgtcaaacgc accctggcgg caaaatcagc cacctttttt cgcatcaaca aaacgcccaa
     1921 aacgctgggt aaagcgtgca acattacctt ctgaagcgac agttctcagt ttacctgtat
     1981 aaaacggatg cgacttagaa gaaacgtcaa ttgtaacata tgggtaagtt acaccatcca
     2041 gttcaattac ccggtcagtt ttgatcgtcg aaccgatcct gaagtactca tcaatactgg
     2101 tatcgtgaaa tactactgta cggtattccg gatggatatt cggtttcaca acagttttcc
     2161 ccctttaagt gaaaattccg cataaaggta catattcaga cggtcagatt caactgtctg
     2221 ttcttataac ctcactctct ttcagtatta caatatgtta tataattata aactggactt
     2281 cccgaaaaaa tccattaaat aagaagcgag ccgttgcgga ggcttgctct ctcctttaac
     2341 ctcatgtgca atatccgtaa cgcgctcaca taaatcatca tctgataata taacaacacc
     2401 cattatttta ttctgaccta aaaacttctt atgaaataaa cgtttacccg acgtatctga
     2461 tcccgctgtt tttacctgta tcctttaaca tacttgagaa gcccgctgaa aaaatatacc
     2521 gtaaaggcgg tttctctgtc ttccataaac ctgtcggata agaagggcat taaatgaaaa
     2581 ccaaaaaagt tatgatgatt gcactggtca gctctgcgct ggctctttca ggatgtggag
     2641 ccatgggcac agcgatcaaa aaacgaaatc tggaagtaaa aacccagatg agtgaaacct
     2701 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
     2761 ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg
     2821 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
     2881 actttaggac aatgggttac cgccgccaga aaagggctcg gtactcctgg ttcccgcacg
     2941 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
     3001 gagcgagata tattaaaaaa agcagctgta gattcaatct gtcaatgcaa cacccctttc
     3061 aattatctct ttcactgccc gcgacggtat cggttatcag tttgacctgc tggctaacgg
     3121 tgaaaccgta ctgcgtgatg catccggcga aaaacgcacc aagggtgaaa aagacggtcg
     3181 tatgctcatg aatgttggcc tgaatgcgga aattcgcgac aacatccgct tcggtctgga
     3241 gtttgaaaaa tcggcatctg gtaagtacaa cgtggataac gcggtgaacg ccaacttccg
     3301 ttattccttc tgataaaaac aaggggccgc ctgccggccc tgatacagag gatgttgtga
     3361 ttacttaccc agcatgccct gctcttcgat aaaggcgatg atgttttgca gaccttcacc
     3421 acttttcagg ttagtgaacc cccatgggcg ttctccgcgc atacgtaaag tatcacgctc
     3481 cattacctcg agggaggcgc ccacgtatgg tgcaaggtca gttttattga tcacgaggaa
     3541 gtcggatttg gtgatccccg gaccgccttt acgcgggatc ttttctcctt ctgccacatc
     3601 tatgacatat atggtaaggt ccgccagttc ggggctgaag gtcgcactca ggttgtcgcc
     3661 gccgctttcc acaaagatca gatcgagatt accaaatttc tcactcagtg cttccacggc
     3721 tgccagattc attgatgcgt cttcacgaat ggctgtatga gggcagcccc ccgtttcgac
     3781 accgacaatg cgttccgctt ccagtgctcc ggcctcagtg agaatgcgtt gatcttctct
     3841 ggtgtagatg tcgttggtga ctacggctaa ttgccaggtg tcacgcatgg atttgcagag
     3901 ggcttccaga agcgcggttt tacctgagcc gaccggaccg ccgacgccga cacgcagcgg
     3961 ttgcttgatt atgttcattt tattttctcc taagaacgga ataatcggga atactgggtt
     4021 tcatgtcgcg cggaagcgat agatgcaagt ggcgttgctg aaccaattgc gtgatccggg
     4081 gttgcaaggg cactgtccat gttcgccgca tagcgggcac acaggcgcat aattaactgc
     4141 tgggcagcca gctggccgta gggtaccagc ctgacgcccg ccatcactgc gctttcaatc
     4201 cagctgtagc caaggctcag ggccaggtct gaaacggata tttgccagcg tacgccgagc
     4261 catgccatac cggttaactg gctttgctgg catagtttgc gccattcggc cgggcagtcg
     4321 ggctgccagt cgaccagaag tctggtgaag gctgcaccgc ggttacgttc ttcatcgcgt
     4381 agctcacggg tttccctgca ggccagcaga taagcggtcc agcgcccggc actgtcagga
     4441 tcgcccgctt cacaggcccg gtatagccgg acaagcagtg gcaggtcgac ggcaaaaaaa
     4501 ctctgttcca tctgcaactg ctgccagtgt tcaaaggcgg cgctatcttc tacccagcct
     4561 gcttccactg cccactccag tccctgggac cagctgtagc ctccaacagg gaggctgtta
     4621 ctggcaagct gcatcagccg cagacgtttt tccggagtcg gcattcagtg gctgtgctcc
     4681 tgatcgtgat tatgggcgtg cgacttgctg gcgtaagcgc cggcttccgg ctcaaacgga
     4741 agatgtgcaa aatcaacatc cagcccaaac tggcgcagca tatcatcgag gacgtgatcg
     4801 tgatggtagc gcagctctcc gggcattatt tgcagcggaa cgtggcgatt gccgagatgc
     4861 cagcaggctt tcgcgagcat aaaaggatct gcgcagcgca ctacagaaac ggcttcatca
     4921 gcggcgatca ccttaatgaa ttcatcaccg ttttcgttac tgagaatatc accatcgcgc
     4981 agcagcagac cgcgtggcag caacagtcca gcctgacgtc catcgttaag ggtgacttta
     5041 atacggcttt ttacccgcat gtcaacaggg agggtaacgc tggcagttgt ctgagccggg
     5101 gtctccaccc gccgggtcaa ataaagcatt tgtgctcctt aaaacaggaa ataacgttgc
     5161 gccattggca gaatgtccgc cggttcactg gttatcagtt cgccgttgat acgcacttcg
     5221 taggtttgtg aatccacggt tatatcaggc aacaggctgt tgtggcgcat atcggctttt
     5281 tgtactgtgc ggcagccttt taccacagca gttgtgctgt gcaggttaag ctgttcagcg
     5341 acgccatttg ctgctgctgc ctgcgacagg aaagtcacac gacagcggtg acgggcactg
     5401 cccaatgcag cgaacattgg gcgatagtgc accggctgcg gtgtggggat agagccgttg
     5461 atatcaccca tcggcgccat ggctatcatt ccgcctttga cgatagtcgc cggttttacg
     5521 ccaaagaacg ccggggacca gagcaccaga tccgccagtt ttcccacttc aatcgagccg
     5581 acttcatgag caataccgtg ggttaatgcc ggattaatgg tgtatttagc gatatagcgc
     5641 ttcacgcgga cgttgtcgtt atcaccactt tcttccggta acgggccgcg ctgaactttc
     5701 atccggtgtg ccacctgcca ggttcgtaac actacttctc cgacgcgtcc catggcctgc
     5761 gaatcggacg aggtgaggga gaacgcgcca aggtcgtgca ggacgtcttc cgcggcaatg
     5821 gtttcctggc gaatgcgcga ttcggcaaag gctacgtcct cggcgatatc cgggtcaaga
     5881 tgatggcaaa ccatcagcat gtccagatgc tcatcaatag tgttgacggt atagggtagc
     5941 gtcggattgg ttgaggaagg cagaatattg gggtgcgcgc aggcggtgat aatatccgga
     6001 gcatgaccac cacctgcacc ttctgtatgg aaggtatgga tagtgcgccc gccgatggca
     6061 gtcagagtat cctcaacgaa tcctgattcg ttaagcgtat cactgtgtag cgcaacctga
     6121 acgtccattt cgtcggctac agtcagtgcg cagttgatta ccgcaggtgt agctccccag
     6181 tcttcgtgaa ttttgaggcc gataaccccg gccgcgacct gctcacgaag cgcatccgga
     6241 ttggagcaat tgcctttacc cagcaacccg atattgaccg gcaggctgtc tgcagcctgc
     6301 agcatttgat aaatgtacca tggtcctggg gtacaggttg tggcgttaga acccgctgtc
     6361 gggccagtac cgccaccgat catggtggta atgccggatg tcagcgcttc ttcagcctgc
     6421 tgtgggcaga tccagtgaat atgcgtatcg acgccacctg cggtaacgat cctgccttct
     6481 gcggcaataa tttccgtgga tacgccgatt gggatagtga cgttgggttg tatatcagga
     6541 ttaccggctt tgccgatagc aaatatcctt ccatctttga cgccgatatc ggctttaacg
     6601 atcccccagt aatcgatgat cagggcattg gtcagcacca gatcagcgca gccggcggag
     6661 agcatttgcc cctgtcccat accgtcgcgg attactttac cgccgccgaa tttgacctct
     6721 tcgccgtagg tagttaaatc atcttcgacc tcgatccaca gctcagtgtc tgccagacga
     6781 attttatcac cggtggtagg gccgaacatg tcagcatagg cctggcgtga aatattactc
     6841 atcatttgcc tccagctcac ccattatatc tccgcggaaa ccgtgcacga tacgggcacc
     6901 gctcagcgcc accagttcaa cctcacgctt ttggcctggt tcgaagcgca ctgcagttcc
     6961 ggcggcgata ttgagacgat agcctcttgc cttctggcga tcgaacttca gtgccgggtt
     7021 aacctccgcg aagtgataat gggaaccgac ctgaattggt cggtctccgt gattttcaac
     7081 gataatactg caggtagccc ggccgatgtt aagctctata tagccaggtt taaccttata
     7141 ttctccaggg atcatctgtt acctcagatt atcggattat ggacggtaac cagcttggag
     7201 ccgtccggaa aggtggcttc gacctggata tcggggatca tttctggtat gccttccatg
     7261 acctgctcgc gactcaggac atgccgtcct tcttccatca gcgcagcgac gcttttgccg
     7321 tcgcgagcgc cctccattat aaaagcgcta atcagggcca cggattcggg atagttaagt
     7381 ttcaggccgc gggccagacg acgctctgcc agcagtgcag cggtaaacag taatagtttg
     7441 tctttttctc ttggagtcag ttccatagcg ttctcttaag tcagccagat ccggggaagt
     7501 accggtgatt tgtcagtcag ttgcggccgc ataaattgcc agatatcacg cattacctac
     7561 tggcaacgca gattatcatc acttaaaaag cgaactgtca gtagaccatc ggtgagcgtg
     7621 gctccggcgt agttttccag tggtgtgagt ttttcgcgta tgtcttccag ttgcgtttca
     7681 ttccccaggt agaacagcat cgttcccacc catggtttcc gggcaacgca ggtaagttct
     7741 ccattggcca cgtgcagacg ctcaatcaac agcggagaac cgtcgaccca tatctccaga
     7801 cggttagcga gtgttccgtg gctgaaggtt tctccgatga ccggacgtcc aaggcacaga
     7861 agatcccagg ccagcagtgt actggaggac gacagatgaa acacggtatg caacgtggcg
     7921 tgagcgccgg gaaaaaaaat cgaatcctgt ggcaaccact ccagcgttgc gttattgcac
     7981 agcgtcaggt tttgctgaag gtgcgcctgc gcgccgctgc tgcggtaaaa tttactggca
     8041 ccaggcatgg tgatcagcgt atggcaatca gagtcaatta ttgcgttgat gatgagttcg
     8101 tcaccgccga cgataccacc gggtggatga agcagacaga ggtggcaggt ttcttcttcg
     8161 ggataaaaag gacgctgaac agtgagcgga ccgacatgat gacgggtggt gagcgtggtt
     8221 ttcccgtgca gacgctgaaa acggagatcc agcatggcct gccagccttt tttattttgt
     8281 ggtaacgcgg tactgagcaa tcttacgccc ctgataaata aagataaaac gacagtaaag
     8341 agccggattt ccttgttata taaaagatga tgcctttcca gtgagttttc gtctgaataa
     8401 tacgaatgtt tgttttcgtc atgaagcgtt ccggctcccg gggctataca tcgccagaaa
     8461 agcaatgcat attgtcggga ttatccagag aatcaggcag aaagatccga caatgggctg
     8521 tgattatgcc atctggctca gaatgatatc aaggtgttat ggggataact tgctgataca
     8581 atagcagtat gacagattca ttaatgcgga aactgctcac tgaaacagag tgttcatgac
     8641 tgagcactgg tcgggtcatg cagatgataa tttccccttt tcttggggaa gcgacagtat
     8701 tttcatgcat gcctgtcttc agtcatacgg ggagagcatc tgtgatacag tccatacatt
     8761 tgttgcggac gtcgctcctg agtcactgac tggtctgcaa acagtttatc tgtacacggc
     8821 agggcttctg tgtgatatcg tttttcatat cgaggggggg cctccagaga tgaatggaat
     8881 aataatattt cctgatgata taatacctta gagaa
//
LOCUS       DAZUFB010000147         8869 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZUFB010000147 DAZUFB010000000
VERSION     DAZUFB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.147"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..283)
                     /locus_tag="ACHY3M_004439"
     CDS             complement(<1..283)
                     /locus_tag="ACHY3M_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4319218.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRIS"
     gene            complement(295..690)
                     /locus_tag="ACHY3M_004440"
     CDS             complement(295..690)
                     /locus_tag="ACHY3M_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4319219.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(732..1757)
                     /locus_tag="ACHY3M_004441"
     CDS             complement(732..1757)
                     /locus_tag="ACHY3M_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4319220.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(1813..2145)
                     /locus_tag="ACHY3M_004442"
     CDS             complement(1813..2145)
                     /locus_tag="ACHY3M_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4319221.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(2155..2733)
                     /locus_tag="ACHY3M_004443"
                     /pseudo
     CDS             complement(2155..2733)
                     /locus_tag="ACHY3M_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            2740..3021
                     /locus_tag="ACHY3M_004444"
                     /pseudo
     CDS             2740..3021
                     /locus_tag="ACHY3M_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3037..3113)
                     /locus_tag="ACHY3M_004445"
     tRNA            complement(3037..3113)
                     /locus_tag="ACHY3M_004445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3077..3079),aa:Arg,seq:tct)
     gene            3356..3988
                     /gene="fimZ"
                     /locus_tag="ACHY3M_004446"
     CDS             3356..3988
                     /gene="fimZ"
                     /locus_tag="ACHY3M_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFS4319222.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(3991..4506)
                     /gene="sfmF"
                     /locus_tag="ACHY3M_004447"
     CDS             complement(3991..4506)
                     /gene="sfmF"
                     /locus_tag="ACHY3M_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFS4319223.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4517..5516)
                     /gene="fimH"
                     /locus_tag="ACHY3M_004448"
                     /pseudo
     CDS             complement(4517..5516)
                     /gene="fimH"
                     /locus_tag="ACHY3M_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(5558..8167)
                     /gene="sfmD"
                     /locus_tag="ACHY3M_004449"
     CDS             complement(5558..8167)
                     /gene="sfmD"
                     /locus_tag="ACHY3M_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS4319224.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGGGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(8198..>8869)
                     /gene="fimC"
                     /locus_tag="ACHY3M_004450"
     CDS             complement(8198..>8869)
                     /gene="fimC"
                     /locus_tag="ACHY3M_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS4319225.1"
                     /translation="LMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIRNSH
                     TNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWM
                     NVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSRSGNYINV
                     HNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVR
                     EVNLN"
BASE COUNT         2271 a         2291 c         2089 g         2218 t
ORIGIN      
        1 gagaaatccg gtctatccag aagttttcct caccgatggt cagcgtgtcg ccgcgccgca
       61 gctgccgcac atcatcagtc cggacaaaca gggacgggct ggagccttca acgcgcacgc
      121 cctgtccggc atagctgata ttttcagggt catcaaaaac accacgtatc acagcaccgg
      181 actgctcacc ggatgtcatg gtggctgacg ttcccatgta cccgcgtatc gtttcatcgg
      241 cgcaggcaat ggcagcatcg aacaggttat cgaaatcagc cacagcgcct cccgttattg
      301 cattctggcc aggccgcgct ctgtcatttc agctgccaca ccggcagaga cacggaacgc
      361 cgttcccggc agcacaaatg ccacagcctc atcccgcgtg gcgtgaagtg catcagtatg
      421 cagcgtcacc agtgccacaa ccgtgaccag atcagccgta tcagtcacgg tatccggctg
      481 cgctgataca acctcatttt catgtccggt cagcgcattt tccgggctga cagatgtgtc
      541 ctgaccggca gcgtcatccg tgtcatcaag ctcctcttcc agctctgcca cacggagtgc
      601 cagttcttct ttcgtccccg tcaggctgac atcacggttc agttgctcac ccagcacctg
      661 aagacgggca atcagttcat ctttcgtcat ggactcctcc acagagagaa aatggccccg
      721 aagggccatg attacgccag ttgtacggac acgaacgcat cagggtcagc cagcagcatc
      781 agcggtgctg actgaatcat ggtgaactca cgcgccggat cgccggtggt cacccagttt
      841 ttcgggtagc gggcagaggc gttaatacct tcgcgctgtg cgtccgcatc ctgaatgcag
      901 ccataggtgc gcagaccgcg tgcctgagtg ttccccagca ccatcgtgtt gtccggaagg
      961 aagttctttt tgacgtcgtt ttccacgtac tgtccggaat acacgacgat cgccgtatcg
     1021 ccatacatcc ccttatagga caccgcttcg cccaggtctt ttaccgctgt ctccagctcg
     1081 gaatgagagc cgcgacgggt atccagcttc tccctgacgg ctttgaagga acggaacagc
     1141 gcccagcctt tcggatcaaa cacgatgata ttcaccacac cgctggcgtt cagcgcgtag
     1201 gcttcgatat cgtcggtcgg gtcatacgtg gacttgtcac gcttgctcca ctccgtaccg
     1261 ccggactgcg tgatgttgtt cgccacactg cggcccatat ccacttcaac cggatcgaag
     1321 gcttcaccgg tcatggtgta tttgccctta agcacggcag aaactgcctg catctcttcg
     1381 acctgagcaa tggccagctc ttcgtctcgc atgttctgca ggatgatgcg acggcggcgg
     1441 taagccgggt ccgccagatt ctgtggatct tcatccggca ggcgacgcag ggtcatctgc
     1501 ggattcactt catgcttggg tttgacatat cccggcgtaa attcagaggt ggagccgcca
     1561 cgggagcgga taacctcacc ggaaacaatc ggcgaaacgt acagcgccat gtttaccagt
     1621 cccggaattt gtgagagata gactttctcc gtggtgaagg gatagctctc acggaaaaag
     1681 agacgcagaa acagcggatc aaacttaaat ttctgctcat ttgccgccag cagctgggcg
     1741 gttgtgtaca tcgacataaa aaaatcccgt aaaaaaagcc gcacaggcgg cctttagtga
     1801 tgaagggtaa ggttaaacga tgctgattgc cgttccggca aacgcggtcc gttttttcgt
     1861 ctcatcgctg gcagcctccg gccagagcac atcctcataa cggaacgtgc cggacttgta
     1921 gaacgtcagc gtggtgctgg tctggtcagc agcaaccgcc agaatgccaa cggcagcacc
     1981 gtcggtggtg ccatcccacg caaccagctt acgggtggcg gtgtccagca tcagcggggt
     2041 cattgcaggc gctttcgcac tcaatccgcc gggcgcggtt gcggtatgag ccgggtcact
     2101 gttgcccagc ggctggtaat gggtaaaggt ttctttgctc gtcataaaca tcccttacac
     2161 tggtgtgttc agcaaatcgt taacggcatc agatgccggg ttacctgcag ccagcggtgc
     2221 cggtgccccc tgcatcagac gatccagcgc agtatcactg cgcgcctgtg cactctgtgg
     2281 tgctgcggcc agaatacggc gggccgtttc cacggtcata ccgggggttt ctgccagcac
     2341 gcgggcctgt tcttcgcgtc cgtgagcctc ctcacagttg aggatcccca taatgcggct
     2401 gttttctgcc gcaaccgcag cggtgatctg cgcgttcacg tccggctgcg ccgcgctggc
     2461 gttttcgccc tccatcgctg gcaccacgcc agtaacgtca gcctgcgaag cagtggctga
     2521 aacagttgtt gattgagtct ctttggtcat tcgccctcct gagagacggg atttacgtgc
     2581 atccagtgca tcacgcataa cggtgatcgc atcggtgctg ttgacaagtt catcagccag
     2641 tccggcatca atggcctcct gaccgctgta cactgcagcc tcggtatcca gcacagcctg
     2701 cacggacagg ccggtatatg ccgacacctt ctgcggttca gcaagacatc atggctatca
     2761 gcttgtcgtc gtgcaggaat tgaagatttc cgtttccatg acctcagaca cacctgggca
     2821 agctggctga ttcagtcagg cgtcccatta tcagtgcttc aggaaatggg cggatgggag
     2881 tccatagaaa tggttcgtag gtatgctcac cttgcgccta atcatttgac agagcatgcg
     2941 aggaaaatag acgacatttt tggtgataat gtcccaaata tgtcccactc tggaattatg
     3001 gaggatataa agaaggcgta actgattgaa ttgtaatggc gcgccctgca ggattcgaac
     3061 ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg cgcgttgata
     3121 ccgcaatgcg gtgtaatcga gtgaattata cggtcaaccc ttgctgagtc aatggctttt
     3181 gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt tcgctatagc
     3241 ggcaaacaaa cacacaccgc tgcgcgtctg aatcaagaaa acccgtattt tcatgtatca
     3301 aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg taactatgaa
     3361 accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta ttgaagttct
     3421 gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc gcataaccat
     3481 cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact tgcccggaac
     3541 agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga aagtgttatt
     3601 tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg gtgctaacgg
     3661 ttttgtcagt aaatgcaatg atcagaatga tatttttcat gccgttcaga tgatcctctc
     3721 cggatacacg ttttttccca gcgaaacgct taactatata aaaagcaata aatgtagtac
     3781 gaatagttca acggtcactg tgctatctaa tcgtgaagtg accatattac gttatctggt
     3841 tagcggatta tctaataaag aaattgccga taagttatta cttagcaata aaacagttag
     3901 tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag agcttatcga
     3961 ctacgccaaa ttatacgaat taatataata ttaattataa ttgatcataa atatcgcatc
     4021 cgctttcgcc acacctggcc gaacaccgct ggctaacgct cgataattgg caaaaaagtt
     4081 tagtgtgaca ttgccatttg catctacttc ctcagtcggg ctcgcctccc ccagtgcgag
     4141 ccgggagcga tcgctattac gtaattcgat ggcgacggtt tgtgccattg cgggatcatc
     4201 cagagccagc aggttggtat cggatgccgg cgttcccgta aataaaatcg caactgaacc
     4261 cggaggacat ccctccagcc gcaggctaaa agggacgagt gccgtggtat cgccagcgtt
     4321 cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc ttatcaatat ccgctgtgtt
     4381 tacggtacag gagaaatcaa caacgttacc gtgcaaattg atattaatcg ttcctaaagg
     4441 gtcagctgcc catccaatgg aactccacag taacccgcag aaacagctaa agagtactct
     4501 tctcattctc cgtacctcat tgataatcga cgcgtaaata cccctgcgcg ctaaacggcc
     4561 cctcggtcgg tttttgaccg gtaatactga taggccaggc gcgaagtgtg aaccgccccg
     4621 ggtttcctgg agacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
     4681 agatcgttag gcgtaatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
     4741 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
     4801 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacgcca
     4861 gctggtcgat tccccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
     4921 aattcaacaa tctgcccggc atttaattcg cagttttgcg gcacttcaac ccggccacca
     4981 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     5041 gtaaacatca cctgtctggg gatctccacc atgttaataa agggacgaat aacctttaaa
     5101 cgaaatatta aacgagaatc catcaccccg aatggaaggt tttgcgaaac gttaggatct
     5161 gttcccatcc gaatataatt ttgcgggggg aactgaatat ccatcacact atcaaccaga
     5221 ctcatcgcac ctaatagata atcatgtaat tgcatatatt tataattatc gatagtttcc
     5281 tgaacaataa agttggtgac ataactacgg tatgtataat tcaccagcgt accgggtgga
     5341 caaattgctg agacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
     5401 tggttattgg tgctattaaa gatatttgag agatcatagt gttcatcttc tgctacgcca
     5461 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc
     5521 aataataatc tgcagattat cttattgata atttccttta tgagccagga tgtgtgcaaa
     5581 ctgccgaaat aacagtgacg gcttgctgta agctttgctt cggcaataca tagtgcgcaa
     5641 tgcagcgtga gttcgcgccg tctccccact gaaccagtaa ttcaccagac aatggcgcac
     5701 cacttaaata aacttgcccg tcatcaccca ccatactggt tattccggta ctgttttccc
     5761 gtaccagtga tccaaacggc accggttttc tgccctgggt aacggtaatg agcgcccgca
     5821 cgcctatgcg ggtatcaaaa ttggcacgaa ccaacgcgcc ttgcgtcggc acaacgctgc
     5881 taatattttt ttcaacatcg atggaattcc ccatcgtatt ggtatcaagc gcgatacggt
     5941 tataccgata aaccgtggca tacggcatca ccgcatagcc gcgccagtcg gttaaaatgc
     6001 cagtttgatt ttcaatgcgt acaccgcctg cgccaggcgc tttaatcaaa acattggtat
     6061 cccctaaagg ctggctcagc gttatgccat tttcatgccc gaccacaccg ccagacagct
     6121 gccagttaac gtcatgttga tcgcggtcgt agttataccc gccccccagc gtaccgtaag
     6181 cggcctgcca gtttgccgtg gcgctgcccg tatacccatt attcgaggta tcaccctggc
     6241 ttacgtgata actcaggttg tggccttcca gtaaggtccc acctacacct gccagccagc
     6301 tattttgccc gttgctgtta cggttggcgt taaaggaggc ataagcgcgg tcgagcgcat
     6361 tttcccgggt gtagcgacgt ttggtcagaa cattgaaagg cactgaaaca ttaagtccga
     6421 caatacgttc gttatcgggg atccctacag attcattcca cgaaaatgag agtgaataac
     6481 tgatgccaac ccagctgctg gtatacccca cctgatacca ggtatctgaa tccgaagtat
     6541 tccagtattt ttgatgggta ccagagatat aaagcgagcc aaagtcatta agtgattgtg
     6601 aaatatttaa ctgcaaacgg tctttacggc taaagcgtaa attgtggtaa ttcacgatta
     6661 tcggttcatc gcgatgctca ccgtcgtaat cgtaatcata ttcgtacccc tccattcgac
     6721 gatacgcaac atcatcaagg gtataaaaac cttgtgtcga atagcggtaa cccattaact
     6781 gaaaattggt gccgaaggtg ttcatcgatt tcgcatagag gaagcgaatg gaatccccct
     6841 catgacgact gtcgtcggct aactgactgc gcgcatgggt cacatccagc gacactgtgc
     6901 cccagttccc gaggttgcgc cccagcccta ataaaaaggc ggtgtaattg gcagataatt
     6961 gcgtcccgcc gtaggcagta aattcctgtg gtaaaccgcc gagtgccgta ccctgaaaaa
     7021 agaaaggcga tgattgctga ctattaccgc tacgaaaatc gcccgccgtc aggtcaaatt
     7081 tgaaacgccc ttcgcgttgt aaaattggca ctgttgaata cggaattgtg taattctgct
     7141 gattaccatc gcgctcgtcg atggtgacgt ccagatcgcc atttgaagat gtcgggtgca
     7201 aatctgtaat ttcaaaagcg ccgggggaaa catagctttg atagataata aaaccatttt
     7261 gccgaatcgt tagctgggcc gccgtacggg caatcccacg tactgttggg gcaaaccctt
     7321 gctggctatc aggatacata ttatcggaag agtaaagccg tataccacga aatccaacac
     7381 catcgaaaat atcacttcct gtattgccgt ctcccattac cagttcactt ttcagcggaa
     7441 taatggcgcg ctgtacccag gtgccaatat tattccactg ttctgaatga tatccatttc
     7501 cgcgaaaata gttccaggaa ccattgttgc gtaaacgcca cgggccaata ttaatcccgc
     7561 tgagctcact aaaaaaatag ctatcgttac cattacctct gttaccggtg aaattataat
     7621 tcaggagtaa agcaggaata ccttcatccc actcttcagg cggaatgtaa ccgtgagcgc
     7681 tactcaacaa cgctatctga ggaatagtga tgttcaggcg catcgctgca aaatcaaagt
     7741 taattgtggc atcaggtaca gctttcagta aattgatgca tttattgttt tgctgctggg
     7801 ctaactcggg aaatgcactg ctattaaggc taattcgttc aaggagtacc tggttaaaac
     7861 agggcattaa cccacctgat ttatcacctg tattttctgt tgtcgattca aatacgatat
     7921 cctgtgaacc aatgaactca tcattacgcc agagatctac ccgatacacg cccgcaggtt
     7981 gatgattacc tctctcaaag cgcgataaat cagcaacgga tgtgccattc tctaataaaa
     8041 atgccgggtt gaaataactt tccgcatagc ttaaaaagga gggtaaaatg gcaagagatg
     8101 aataacaaat tccggccaga caccaggtat acctctgcgg aatatccgta gtagtgggta
     8161 ttttcataat tagctctgat attgtcagat gattcggtta gtttaagttc acttctctga
     8221 ccggggttac tgaaccataa tcattaacgg tctgaaattt cagctttccc tgcactcctg
     8281 agggtaaggg aatttgtgaa ttaactctgg gtgcagccat agcattcccc aacttttggc
     8341 tgcccacttg taagttaacc agagtgacat aaaaaggtga gggattatga acattgatat
     8401 agttaccgga tcgcgaaaac ttgagtgtgt ccggtgcttc tgcgggtaat tcttgtaatt
     8461 gaattagacg gagaaataat ttcatgcgcg ataaaatcgc cagttgcaaa acattcctgc
     8521 cgttcaatgc atttttatct accgaaggga tcgtcttaac attcatccag aacagagact
     8581 cacgatctgc tgccagcggt ggaccggtgt aaataatacg caaggtattt tcgcttttgg
     8641 gttcactaac aaacagtggc ggtgtaatga tgaatgactt ttcttttaca ccgctgctgt
     8701 tttcaatcca cgaattgacc agaaagcgct cattggtatg gctatttcta atccataccg
     8761 cagtctgttt agcatcagcg ggataaataa tacgcgtggc acctaatgcg atccctccgg
     8821 cagcatgggc gctggccgaa atgattaaat aaaatataat gagcatcaa
//
LOCUS       DAZUFB010000148         8851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZUFB010000148 DAZUFB010000000
VERSION     DAZUFB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.148"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..810
                     /gene="csgD"
                     /locus_tag="ACHY3M_004451"
     CDS             160..810
                     /gene="csgD"
                     /locus_tag="ACHY3M_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFS4319226.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            815..1204
                     /gene="csgE"
                     /locus_tag="ACHY3M_004452"
     CDS             815..1204
                     /gene="csgE"
                     /locus_tag="ACHY3M_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFS4319227.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1229..1645
                     /gene="csgF"
                     /locus_tag="ACHY3M_004453"
     CDS             1229..1645
                     /gene="csgF"
                     /locus_tag="ACHY3M_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFS4319228.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1672..2505
                     /gene="csgG"
                     /locus_tag="ACHY3M_004454"
     CDS             1672..2505
                     /gene="csgG"
                     /locus_tag="ACHY3M_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFS4319229.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2569..3060)
                     /gene="ycdZ"
                     /locus_tag="ACHY3M_004455"
     CDS             complement(2569..3060)
                     /gene="ycdZ"
                     /locus_tag="ACHY3M_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS4319230.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3162..3716)
                     /gene="ycdY"
                     /locus_tag="ACHY3M_004456"
     CDS             complement(3162..3716)
                     /gene="ycdY"
                     /locus_tag="ACHY3M_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFS4319231.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3740..4477)
                     /gene="ycdX"
                     /locus_tag="ACHY3M_004457"
     CDS             complement(3740..4477)
                     /gene="ycdX"
                     /locus_tag="ACHY3M_004457"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFS4319232.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4532..5470)
                     /gene="ghrA"
                     /locus_tag="ACHY3M_004458"
     CDS             complement(4532..5470)
                     /gene="ghrA"
                     /locus_tag="ACHY3M_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFS4319233.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5704..5791
                     /locus_tag="ACHY3M_004459"
     tRNA            5704..5791
                     /locus_tag="ACHY3M_004459"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5738..5740,aa:Ser,seq:gga)
     gene            complement(5941..6783)
                     /locus_tag="ACHY3M_004460"
     CDS             complement(5941..6783)
                     /locus_tag="ACHY3M_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4319234.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6868..7065)
                     /locus_tag="ACHY3M_004461"
     CDS             complement(6868..7065)
                     /locus_tag="ACHY3M_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS4319235.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7077..7568)
                     /locus_tag="ACHY3M_004462"
     CDS             complement(7077..7568)
                     /locus_tag="ACHY3M_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4319236.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7565..7939)
                     /locus_tag="ACHY3M_004463"
     CDS             complement(7565..7939)
                     /locus_tag="ACHY3M_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS4319237.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7986..8360)
                     /locus_tag="ACHY3M_004464"
     CDS             complement(7986..8360)
                     /locus_tag="ACHY3M_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS4319238.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8523..8744)
                     /locus_tag="ACHY3M_004465"
     CDS             complement(8523..8744)
                     /locus_tag="ACHY3M_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4319239.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT         2289 a         2176 c         2149 g         2237 t
ORIGIN      
        1 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
       61 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
      121 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
      181 attcatggtc atacattatt gttgatcact aaaccgtctt tgcaagcgac agctctcttg
      241 cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
      301 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcaga taaaaagctt
      361 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      421 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      481 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      541 gaatgctact ttacgcaaaa gcttgccagt tacctgatta cacattctgg taactatcgt
      601 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataagctg
      661 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      721 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      781 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      841 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      901 attgttaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      961 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
     1021 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
     1081 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
     1141 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
     1201 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
     1261 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1321 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1381 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1441 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1501 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1561 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1621 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1681 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1741 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1801 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1861 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1921 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgccaggg cttacaaaac
     1981 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacggt tgccattaat
     2041 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatta tggttgaagg ttcgattatc
     2101 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     2161 gacacgcaat accagctcga tcagattgcc gtgaacctgc gcgtcgtcaa tgtgagtacc
     2221 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     2281 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2341 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2401 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaacgac
     2461 attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaaagccg
     2521 gaattatcat ccggcatttt actgtttaag cgaaagcgtg agtgcccctc acgctttatt
     2581 tttgatttcc tgctcacggt gagccgtctt tgcactacgc gccgccagcc acaggccgct
     2641 gtttttcatt gcgtaaccaa ataccagccc aagcgccagc gaaggtaaca ccagtttcca
     2701 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag
     2761 cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc
     2821 gagaatttcc agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac
     2881 gccactaagc agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaggc
     2941 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
     3001 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
     3061 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
     3121 aacaaattat ctgttgcagt tatgagtgac gatgatcaca tttactcttc agaatcttct
     3181 tccagctcgt cccacattgc actaatggca tcacgggtta gcggtgccat ggtgcgccag
     3241 aaaggcgtgg ttgcatgggc ctccactttg ccgaggaacg caccacacca gggtaacaga
     3301 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
     3361 caggaagccg caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc cagtggcatc
     3421 cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta
     3481 cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
     3541 tgcgccatat cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag
     3601 ttcgcagcca gtttcccctc acgaatcagg gtaaacagcg gcaccagtaa aggatcttgc
     3661 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat
     3721 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
     3781 attcaaggaa gttcagtaag cggcgcggcg aaacattcaa aatgcgctct ggcggaaaat
     3841 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag
     3901 aatccgagcc taatgccacc caacctccag catcacgtac cgctgcggct accgcacgac
     3961 agttgtcttc actgccctta cgtgagtgta aaaatgaaga attattgatt tccagcgcca
     4021 cctgatgttt cgcggctgct tcagcaacgg ctttcacatc tatttcatat ttgggatttc
     4081 cgggatggct tattatatgc acattgccgc tggcgatagt ggcgatcatc gcttgtgtat
     4141 tggtcgcttt gtcatgtggt gcaaaaaccg gctcatgaaa accggcaata atcagatcca
     4201 gcgagtcaaa cattttaccg ctgcaatcaa tttcaccatc aacattttta atgttagctt
     4261 cgatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga
     4321 agtgccagtg atgcggagca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt
     4381 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc
     4441 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
     4501 cgccaaggat agcaggaatc ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc
     4561 cgcagaccct ctcccctttt tcgagctggg caatggtgcg agaaatgtac tccacagctt
     4621 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
     4681 gcggactttc aggcggtaag ggttcacgat taaaaacatc caacattgcg cctttaactt
     4741 tgccgctatc cagcgccgcg agcaggtcat cttccacaac atgaacacca cgcgccaggt
     4801 tgaggagata cgcgccatcc ggtaattttt cgagtaattg ttgattaata atgccgacgg
     4861 tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca
     4921 gttcttcccg tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
     4981 aacgcagcgg aaagcgccag gtttgcagac tctgagcaac tttactgccc agtacgcctg
     5041 cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
     5101 aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
     5161 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
     5221 aagggttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca acaccggccc
     5281 cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccaga
     5341 ctaaagcata atcagcagag tcattatctc cgcttttcca cgctctgact cttgcctgag
     5401 gaatagcttt gcgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga
     5461 tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg
     5521 atctacaaag gccaccagca taacaaatcc gtggtcggtg gcaaaagaag cagatttcgc
     5581 ttattaaaac cacacattga ttgaagtttg aataaacgcg cgattttttc aaaaagtttg
     5641 ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca ggaacgacaa
     5701 tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat acggcaacgt
     5761 atcgggggtt cgaatccccc cctcaccgcc agattataag cgcatgttat tgtttaacat
     5821 gcgctttttt attttccctt ccagacagta tgccatcggg tatgccatct gaaaccggtt
     5881 tatatgcccc gcgatcatat cttatcggac aatttccgat ctttgagcat actggcaaag
     5941 ctatactgcc agcatatcag gatagtgttt tgcgataata tcattcagtc tgtcaaccag
     6001 ttctggtttt ctgaacgtga tatgcgcgga gcccttctta aagtatctga tgctgaacat
     6061 ctcatcttca tagctctctt taccctgaac agactgaatg tgatcatcca gacgaagggt
     6121 gatgtttccc cggttgtcgg ggattggttt accgctgaac aggtgcaaca ttctttccag
     6181 atcggcgagt cggtcagcct gttgtccggt gataagatga aatccccacc ggtcccatct
     6241 caccagattg ttgacgataa ttttactgcc aaatttacag gggcaattgg ttttgtaatt
     6301 ccagttcagc cctctgaaga cgttgatcac ccctcgctca aacacctcat ccttattctg
     6361 gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg atttccggaa aattatcgta
     6421 ctccagtgac cggtaccatg tatcacgggc ctgcgtatcc attaatgaca gcatcccgga
     6481 acgttgcatc aggtcacgcc agatttcgcg atcaagattc cgggtaatgg ctttcattgc
     6541 tgtttcgggt ttctccatca gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc
     6601 cggggcagtt ttacctccga tgctttccgt cagcaccgag atttcagcca gttgtcggat
     6661 aagtgtgtcg atttgcaaca gtgtactctt tcgtacagcc agaatgtggc gaatgctggt
     6721 tgagcagata acgtccgtat gcccgttgac cacttccgga cgggagattg tgatatctga
     6781 cataatcgat attccttttt gcagataaat gaaaacgcca gccctgtgca ggctggcgtc
     6841 agaggaatga agtctggtga gtgagcttca ccggttctgg tgcagaagct gcagatgacg
     6901 cagggtacgg acatcctccc gggcctgttc gatacagggc aacagtgctg ctgaatccgt
     6961 tttatcctca tcgttgtcaa agataattcc cgatccgcag tcgatattgt cctgcagcca
     7021 cgcaatcaga atatccagcg ctgtttccgt ggttaatgat ttcatgttgt tcctggttac
     7081 cagtcgaaag tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg
     7141 acgccataac gccggatgag tgtgacgacc agacgacgga acgtttttga cagccccagg
     7201 cgtttaagcc gcagaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct
     7261 gtaaaatgaa tccattctga ttcaccgaag tcactggtct ggtgtgacag cgagtacaac
     7321 caggcgttgt ccgtttccgt gatatgtgcg gtactgcagc gtatgccggt aagggtagca
     7381 aatggtggcg tgcattctgc tgaggtctgg tcagattcat caacaacacg aagggagtag
     7441 ccgttatcgc agaccatgtt aatcagttca gcgagctcaa taccatcgac gtcaacgaca
     7501 atgcgcccca tgttcagtgc ctgtacgtta acgctgtcgg cttccagtgt cagggccagt
     7561 ttcatcattt cgcctccgga tacttaccca gggtaatgtt atttaccgtt ctgtaattgt
     7621 cgcgggtcat caggccggtc gcccggcgag cccggaggat atcaatactg tttattaact
     7681 gagagcgggg acaggtgctg aatcccggct ggtcggtacg caccagcgcg tatttttcca
     7741 cgagaaaatt aactgcatca cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac
     7801 gttcatcggc aaacggtgtg tcattcagtg tgaggccata atgctggtcc agcagtcgga
     7861 tgagcagtgt ctgccagatg gtgacgggag acgggcagcg agatacctcc cgtacatgtg
     7921 tgtcggataa tgttttcatc ttaagttctc tgtaactggt agtgctaaag gtgagagctt
     7981 cggggttatg aggtggttgc gggtgctggt gtcggataaa cagccagata aacgtaacca
     8041 caggagccga gggtgtcggc ttcgcaggtc agcccttttg cattcagcgt gacggtatgc
     8101 tgatatcggg gattcagttc accgctggtg agcatgagtt ccagttgttt catcagcagc
     8161 ggaaaggcct ggtccagatg gtatgcatct gcgtcgctga agcggcctct gataccggcg
     8221 cggtcggcaa ggtaatgcag gcggttaccc tcttgcacca gacgagcgcc aaaacagggc
     8281 ctcactgtgc agggaagccc ccaccagggg cggtcgtgat tgtcatcggg atgagttatc
     8341 ccggagagtt tgtctgacac gataaaatcc tctctgaaaa gtggttaaga atggtttaac
     8401 gatgacaggt cagccggcgc gctgaccggt ttcggtgaag atgtatgcat tgatgagaat
     8461 ggcctcatcc acagcggcgt ctgaggtcat ctggtcgtac tcgtttcccg ggtgacggaa
     8521 catcagttca gggtgacact catcagccgg acatacgggc caaaactgtc cttacggcgt
     8581 tctgcaaaca cggcaagaac gccgggaata tcctgcactt cacgaccagt atacgcttca
     8641 gcactgccgt gccagcgata cttaccggta cagaacggaa aaagacggga tgcaggatgc
     8701 tgttggtgaa tacgcatggc ttcaccacgg gtgatgattc tcatgatgtg atacctctga
     8761 agacagaaga taaaagtgaa aacaggtgtg atgtggttgt gacgggttaa agcagaccat
     8821 gttcggcaaa ggagaaaacc tggttgccac c
//
LOCUS       DAZUFB010000149         8837 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZUFB010000149 DAZUFB010000000
VERSION     DAZUFB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.149"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..305)
                     /locus_tag="ACHY3M_004466"
     CDS             complement(3..305)
                     /locus_tag="ACHY3M_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319240.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(302..928)
                     /locus_tag="ACHY3M_004467"
     CDS             complement(302..928)
                     /locus_tag="ACHY3M_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFS4319241.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1132..2403)
                     /locus_tag="ACHY3M_004468"
     CDS             complement(1132..2403)
                     /locus_tag="ACHY3M_004468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4319242.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPRFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2403..2840)
                     /gene="umuD"
                     /locus_tag="ACHY3M_004469"
     CDS             complement(2403..2840)
                     /gene="umuD"
                     /locus_tag="ACHY3M_004469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS4319243.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRGEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(2837..3085)
                     /locus_tag="ACHY3M_004470"
     CDS             complement(2837..3085)
                     /locus_tag="ACHY3M_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4319244.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIERRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3503..4405
                     /locus_tag="ACHY3M_004471"
     CDS             3503..4405
                     /locus_tag="ACHY3M_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFS4319245.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQIG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            4402..4713
                     /locus_tag="ACHY3M_004472"
     CDS             4402..4713
                     /locus_tag="ACHY3M_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319246.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            4790..5473
                     /locus_tag="ACHY3M_004473"
     CDS             4790..5473
                     /locus_tag="ACHY3M_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFS4319247.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKHAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            5474..5695
                     /locus_tag="ACHY3M_004474"
     CDS             5474..5695
                     /locus_tag="ACHY3M_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319248.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            5709..6143
                     /locus_tag="ACHY3M_004475"
     CDS             5709..6143
                     /locus_tag="ACHY3M_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFS4319249.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            6189..6965
                     /locus_tag="ACHY3M_004476"
     CDS             6189..6965
                     /locus_tag="ACHY3M_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319250.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLAWDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            7383..7808
                     /locus_tag="ACHY3M_004477"
     CDS             7383..7808
                     /locus_tag="ACHY3M_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4319251.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGQDFLYAEASVYALAKQMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            7855..8274
                     /locus_tag="ACHY3M_004478"
     CDS             7855..8274
                     /locus_tag="ACHY3M_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFS4319252.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRLMRQALDGENG"
     gene            8274..8465
                     /locus_tag="ACHY3M_004479"
     CDS             8274..8465
                     /locus_tag="ACHY3M_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319253.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            8536..>8837
                     /locus_tag="ACHY3M_004480"
     CDS             8536..>8837
                     /locus_tag="ACHY3M_004480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319254.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAP"
BASE COUNT         1907 a         2367 c         2438 g         2125 t
ORIGIN      
        1 tgttatcttt gttttacttc tgctatcaat cgtgatacct ctgtttttga tagtgtttta
       61 agtgcttcta ccgccgccct gacaacatca gaacgattca ctctgccttt acctgaaacc
      121 atctcagccg ttataagggc atcaatgtct tttaggtttt cttcaaaaaa agaaatactc
      181 actggttttg ctttgcttac aaccgatgat ttatctgatg actttttggt attatcaagg
      241 ccagccgtgc caccgttaat aaatgctgcc tcgtcacgag gtgaagtgtt tgctctattt
      301 ctcacagaat aacctctttc actaacgctt cgatttcttg ctgggcttta tcattttgcg
      361 tttcaaaaat tgtcattgat tcggcccaag catctcgatg tgctttacga tcacataccc
      421 gggagtttgc cagatgcatt tcagggtaat ccttaagtac ttcagctgcc tcattggctt
      481 cattaacaaa catatttgtg ggcgtcatgt ttaaaacgag atatccccga acatcctcat
      541 taaaatcttt tgctgctgta aaaacggagc atgtatgagg cacaacatcc aggtccatct
      601 gagatgggcg caaaggagaa atgaatacat ctgcggccat caatccacta cgcatttctg
      661 cgctatcacg gccagcacag tctgcaataa caaaatcata ggatttttcg tgttccttta
      721 acatggcttt gatgttacct gtcgcgcctg taaccggaat atgagggaga tcttcagggc
      781 gattgttgta ccaggttaat actgatttct ggtcatctgc gtcgacaata atcactctat
      841 tgcctagtga catcagatac gcagctatag ataccgccag tgtactttta cctaccccac
      901 ccttttgact acctaatact attatcatta tagccccata ttaagttgtt aagttaacaa
      961 cttaatattg ccatctgaac gcgaatcatg caattatttt tgttatatta acaacttaat
     1021 aagttgttaa gtatttgatt tatagattaa ttaagataac aagataataa gataataaga
     1081 taataagata acaagataac aagatagcaa tattatgggt ggattacata ttcataactg
     1141 ggctactggc aaatctgtcc atctggtggt atacgcagga gaaagcattt cccgtttcat
     1201 ttgccactca ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt
     1261 cagttcatcg agtgttttca ttaacttttc actgttttta cggggctgga tttcatcaaa
     1321 taatcccggc tgtgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat
     1381 atagcggtac ccttcacgcc agacatggtt taaggccctg catgcggcgg caataatgtc
     1441 ccggctgtcc tgtgtgggca atgaaagctt ttccacagcg gcattgctgt aacagggttc
     1501 ttttactgca aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgttc
     1561 cccacgtagt ttctctgcag cccgctctgc atactgaaca acagcctggt gcatggcatc
     1621 tttgtctgtg attcgttcac caaaactgcg actacagaca atctgttgtt ttgccggtgg
     1681 tgcttcttcc agggatatgc aggactcgcc gttgagttcg cgtaccgtac gctcaagaat
     1741 gacgctgaag tttttccgta tgaatgccgt gttagcctgc gccagctgca gtgctgtgtt
     1801 aatacccagc gcattcagct tttccgtcag tctgcgtcct actccccaga cctcaccaac
     1861 tggctgcagc cccagtagct tcaagatccg attacggttt tctgccgtca gcgcgaccac
     1921 accggaaaac tgtggccatt gctttgttgc ccactgtgca cttttagcca gcgtttttgt
     1981 aggcgcaatg cccaccccca tggtgagtcc tgtccagctc tttacctgtt ccctgagctg
     2041 atgaccaaaa aactccggag agatgcaatg atttatcccc cgcaaatcaa tgaacatttc
     2101 atcaattgag tagggctcaa ctgcgggaga aagcgactcc agaacagcca taacccgttg
     2161 gctcatgctg tggtacagcg cataattgct ggaaaataca tgtattttct tctccaggcg
     2221 catttgtctc acctgaaacc tgggctgccc cattctgatg ccaagggctt ttgcctccgg
     2281 gctgcgcgcg atcacacagc catcgttatt gctgagtacg atgaccggtt cgttgcgaag
     2341 gtccgggcgg aaaacttttt cacatgaggc gtagaaactg ttgatatcag ccagtgcaaa
     2401 catcagcgta actcccgcgt cctgtgtatc acgtgagtga caacaccaaa aatacagatg
     2461 ttttccggat acagtgtgcg gaattccggg ctgtctgaaa ccggctccag tgccgggcgt
     2521 gggcgcaaca gcagtcgttt gacggtgaac tcaccgtcga tctcagcgat aacgatgtcc
     2581 ccgtgttgtg gtttttcgcc cctgtctact accagcagat caccattctg cacgccagcc
     2641 tggttcatcg attcaccgct ggcgcgcaga aagaaggtgg ctgcaggtct gctgatgcaa
     2701 tagctgttca gatccagttc ctgctcagca taatcagtgg caggcgaagg aaaaccggcc
     2761 tggcaacgat cggcaaacaa tggtcgcacg taactgtcat cgcctgaagg gtcagccgga
     2821 cggtgataaa cggtactcat gctgcaggca gccagctgtc gtcctgccag atttcttcca
     2881 gcagagacag gatacgttct ttatccttgt cgttgcgggc tcctgtgaca gatacagacg
     2941 gggcgctgcc ctggtctata cgcagccaga tttccgggta gtgtggtttc agacgtcgtt
     3001 cgatttcatt ctgtagcgcc tgaagtgtgc ctgatttgag gttttttgtg ctctggcggt
     3061 cgaaaagaat ttcaatgcgg atcatgttct catcttcctt ttcttttgct gtatatatat
     3121 acagtattct aataactgaa tccgcacaca gtcaaatgtc attgttctga ctaaaaaacg
     3181 ctcaattctc tgagggggtg gctgtatgcc tgctgtggag tgctgtgtcc agcttatcca
     3241 cagcattttg tgcacggttc tgtggacaaa atacctggtt acccaggccg tgccggcacg
     3301 ttaaccgggc tgcatccgat gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcacg
     3361 tttcgtcgtt cctcctccac gcgctgcggc ttcggggccg cacctgcatt cgtatgcggt
     3421 cgcccggtta caggtgcggc acggcctgat ggaggccgca tgtgagagga gaattcccat
     3481 gccaaactgg tgctcgaatc gtatgtattt ttctggtgaa ccggcacaga tcgctgagat
     3541 taaacgactg gccagtggtg ccgtcacacc gttttatcgc cgcgccacaa atgaaggtat
     3601 tcagctgttt ctggccggaa gtgccggact tctgcagacc actgaagatg tgcggtttga
     3661 accatgcccc ggactgacgg ctgccgggcg tggcgttgta tcgccggaga atatcgcgtt
     3721 cacccgctgg ctgacacacc tgcagaacgg tgtactgctg gatgaacaaa actgcctgat
     3781 gctgcatgaa ctctggctgc agatcggtac tggacagcgt cgctgggaag gattaccgga
     3841 cgatgtcagg gataccatca ccgcactttt caccgcaaaa agaggtgact ggtgtggctt
     3901 ctggagtaac gaggatgtat cggtgtggtg gaaccgtctg tgtgacaacg tactgccgga
     3961 aaaaaccatg ccgtttgacc tgctgacggt tctgccgacc cgcctggatg ttgaagtgaa
     4021 tggcttcaac ggtggtgttc tgaacggtgt tccttctgca tatcactggt atacggaaca
     4081 gtatggcgtg aagtggcctt gtgggtatga cctgaatatc agcagtcagg gggaaaactt
     4141 cattcaggtg gatttcgaca cgccgtggtg tcagccggaa agcgacgttg ttgcagaatt
     4201 aagccgccgt ttcagctgca cgctggagca ctggtatgcc gaacagggct gtgatttctg
     4261 tggctggcag ttgtatgagc gcggagagct cgttgatgtg ctgtgggggg aactggaatg
     4321 gtcttccccg acagatgacg atgagctgcc ggaagtcacc ggacctgcgt ggatagtcga
     4381 caatgtggcg cattatggcg gatgaagtat gacggagacg ggcgggcaac cgcccgtttc
     4441 ttttccggta aaggatgtcg ccgggctcct ctttttactg cgccggctga cgcggcgcgg
     4501 cataaacgct gcctgtggtc agtgtcccgc gtccggcggg cacgggacgg gcgcttttac
     4561 cgctcccggc tggcgtcggt gacagtgtac gccagcccgt cgcccttttc tgatgaacgc
     4621 ccttcaagcc gctttcgcgg catagccttg ccgtcaaaaa gacgacggct gcggtattcc
     4681 acggtcggcc tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc
     4741 accacagggg tgccctcccg gtgccctctg taagagaagg agcgttgtta tgtcccgttt
     4801 tgtcctcggt aactgcatcg atgttatggc ccgtattcct gataacgcca ttgatttcat
     4861 actgactgac ccgccgtacc tcgtcggttt tcgtgaccgt tccgggcgca ccatcgctgg
     4921 cgataaaacc gatgaatggc tgcaaccggc ctgtaatgaa atgtaccgcg tactcaaaaa
     4981 agacgcgctg atggtcagct tctacggctg gcaccgtatt gaccgcttta tggcggcctg
     5041 gaaacacgcc ggattcagcg tggcagggca cctcgtgttc accaaaaact acacatccaa
     5101 atctgcgtat gtcgcgtacc gccacgaatg cgcttacatc ctggcaaagg gcagtccggc
     5161 ccggccccgt aaccctctgc cggatgtgct gggctggaaa tattcaggca atcgtcatca
     5221 cccgacggaa aagcccgtta ccagcctgca accgctgatt gagagcttca cacacccgaa
     5281 cgcaattgtg ctggacccgt ttgcaggcag cggctcaacc tgcgtcgccg ccctccagtc
     5341 cggacgccgg tatatcggta tcgagctgct tgagcagtat caccgtgccg ggcagcaacg
     5401 ccttgccgcc gtgcaacggg ccatgcagca gggggccgcg aatgatgact ggtttatgcc
     5461 ggaggctgcg taaatgaact atgcaggaca cgaaaaactg cgcgccgaag tggcggaggt
     5521 ggccaatgcc atgtgcgacc tgcgtacaac catgaatgag atggagcagc ggtacagctt
     5581 taatgccgac accctgccgg aacgtctggt gcgtcagacg ctgtttcgcg caaaccgcct
     5641 cctgatggag gcatataccg aaattctgga actggaagcg tgctttaaag attgagaagg
     5701 agacgagaat gtacggaaca tgcgaaacgc tatgccggga gctggcagta aagtatccgg
     5761 gagacatgcc gctgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg
     5821 gaatggatat ttccctgtcc gatcatgaaa tcagaaccgt cctggcgcgc ctggaggaca
     5881 tcccggaaga ccagcggact gaatccggta tttcttccgg cgtggcgatg gagatcatca
     5941 ataacgtgag cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc
     6001 agaccgctga acaggcattg tggaaacgtg aatgggccgc ccgggatcat ggcctcgccg
     6061 tcccggaatg cgtcacccgc cgtcaggcgg tgattaatca ggcccgcacc ctgctgaaaa
     6121 acaacagaca cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagaac
     6181 gattaatcat gaacgaaaac acaacactga acgcactgat ttgtcgtcac gcccgcaacc
     6241 tgctgctggc gcagggctgg ccggaagaga cggatgttga ccagcggaac ccgaaccatc
     6301 cgggctggat cagcatttat gttctgctgg atgcgccccg gctggcgacg ttacttatca
     6361 accgtcacgg cggcgcactg ccgccgctcc tggcctccgc cattcacaaa ctgaccggaa
     6421 ccggggcgga acttgtactg tccggcagtc agtggcagtc gctgccggta cttccggcag
     6481 acggaacgca ggtgtctttc ccgtatgccg gtgagtggct ggcagaggac gaaatcaggg
     6541 cagttcttga tgcggtacgc gatgcagtac gctgtgtcag ttaccaggtg gcagaagata
     6601 cgcggcgtat ccgggcggcg ctgaccacca ccggtcagac gttactgacc cgccagacgc
     6661 gccgctttcg cctggtcgtg aaggagagcg atcacccctg ctggctcgat gaggacgacg
     6721 aaaacctgcc cgtggtgctc gacgccatcc tgaaccgggg cgcacgtttt tcggcggtgg
     6781 aaatgtatct ggtcagcgat tgtattgaac atatcctgtc cagttggctg gcctgggatg
     6841 tgctgcgtat accggatgaa ccgccccgcc gctggtttga ccgtggtgtt ctgcgggagg
     6901 tggtccggga agcccggaac gaaatccgca gcatggcgga tgccctggca aaaatccgga
     6961 aatgatgaca ctggcggggt aaatccccgc ctttttcctg ccggtccccg gacagagcac
     7021 aaaggacgtc gccgccggct ccgctttacc cggccatgca gggcatggcc ttgtggtttt
     7081 tcagttatgt ggccgccgtg tcgtgtgcgg gctgtgccgt gcctccatct tagccgggct
     7141 ggcagggatg caagggtacg cttcgccgct gcggtcaccc ggtccctcct tcccgtctgc
     7201 cgtgattttc cggtcgttca cccgctcaga tttcgcggtc tcaccctcca actcccgcca
     7261 gccgtgtgcg tcaggctgcg gcttcccttg cgccctgtgc atccccacct tatcgcccgg
     7321 cttttatgga ggcacggcac cgcccggtgt cgcggaatat gtgaacaacg gaggaaaaca
     7381 tcatgcaata tgcgaaacct gtcactctga acgttgaaga gtgcgaccgt ctgtcttttc
     7441 tgccttacct gtttggccag gattttctgt atgccgaagc gtctgtatac gcgctggcga
     7501 aacaaatgat gccggaatat gaaggcggat tctggcactt catccgcctg ccggacggtg
     7561 gcggttacat gatgccggat ggcgaccgtt ttcacatggt gaacggtgca aactggtttg
     7621 accgtaccgt gagtgctgat gccgcaggta tcatcctgac ttcccttgtg attaaccgcc
     7681 agttgtggct gtaccacgac agcggggatg cgggactgac acacctttac cggatgcgcg
     7741 atgcgcagtt gtggagccac atcgaatttc accccgaatg caacgcgatt tacgcggcac
     7801 tggactgatt aacggacggg gcggcaatgc cgcccctgta aaggagacga gaacatgtac
     7861 tgtactgtta aagaaattat ccgcgaagtg ctggatacag acgtgccgga cagtgaatgc
     7921 gtttttgccg tggtgctgac ccgtggggat gtgcgtcaca tagcccagga ctggagtctg
     7981 acagacgatg agctggaaac cgtcatgcag cggctggacg atgcctttga gcatggtgct
     8041 gatgtcagca ttgttcacga cgttgttcgt gaactgatgg aggaaaagcg cgtaaaccgt
     8101 cttgtaacag tccctgcggt gctgctggaa aaagtgatgg tgatggctgg tagtgaaatt
     8161 tatcgcctcc atgctgtagg gagcgagaac gggggcgacg gtgacgcatt tgtcagggaa
     8221 gaacgtgaga ttatgcggtt gatgcgtcag gcgctggacg gggagaacgg gtaatgaata
     8281 tcagcacgga aacccgcgaa attctgcgta attacagggc cgtgattaat gcgcggcgtc
     8341 gtgaaatggg gcagaaaccg ctcaccactg cgcagattgt tgatgaaatc tgcgattttg
     8401 tggcgaatca gcaggcggtt ttcctcggtg gtcactatat ccttcagggc agcagaaaca
     8461 ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg cgcgaaaacc cctggcaggc
     8521 tgacgcctgc cggaaatgcg gggtgcagcg cctgcggcgc tgccggcccg gctttccggt
     8581 cagaaggtag tgtatggctg tatgcaggga acaggtgtga tattccgtga cactgtcacc
     8641 gtttctggct gtcttttatt gcgcccactt cggctcccgg ctgcctggcg gcatccggtc
     8701 gtcaggcccg actccgacag gcagctgctg gcgcacccgc cagtctccgc cgaacccgct
     8761 ggcgcggttt cgggctgccg ctacggtccg atgcgtgaaa acccggcttg cagcgaccgt
     8821 tccggtcgcg ccgcccc
//
LOCUS       DAZUFB010000150         8762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZUFB010000150 DAZUFB010000000
VERSION     DAZUFB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.150"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1979)
                     /locus_tag="ACHY3M_004481"
     CDS             complement(<1..1979)
                     /locus_tag="ACHY3M_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4319255.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSA"
     gene            complement(1960..2373)
                     /locus_tag="ACHY3M_004482"
     CDS             complement(1960..2373)
                     /locus_tag="ACHY3M_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4319256.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(2400..2831)
                     /locus_tag="ACHY3M_004483"
     CDS             complement(2400..2831)
                     /locus_tag="ACHY3M_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4319257.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(2845..3597)
                     /locus_tag="ACHY3M_004484"
     CDS             complement(2845..3597)
                     /locus_tag="ACHY3M_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319258.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(3605..4000)
                     /locus_tag="ACHY3M_004485"
     CDS             complement(3605..4000)
                     /locus_tag="ACHY3M_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4319259.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALSGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(3997..4572)
                     /locus_tag="ACHY3M_004486"
     CDS             complement(3997..4572)
                     /locus_tag="ACHY3M_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319260.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(4587..4940)
                     /locus_tag="ACHY3M_004487"
     CDS             complement(4587..4940)
                     /locus_tag="ACHY3M_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4319261.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(4933..5307)
                     /gene="gpFI"
                     /locus_tag="ACHY3M_004488"
     CDS             complement(4933..5307)
                     /gene="gpFI"
                     /locus_tag="ACHY3M_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4319262.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(5359..6387)
                     /locus_tag="ACHY3M_004489"
     CDS             complement(5359..6387)
                     /locus_tag="ACHY3M_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4319263.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGSLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(6445..6792)
                     /locus_tag="ACHY3M_004490"
     CDS             complement(6445..6792)
                     /locus_tag="ACHY3M_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4319264.1"
                     /translation="MVTKTITEQRAEIRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(6829..8184)
                     /locus_tag="ACHY3M_004491"
     CDS             complement(6829..8184)
                     /locus_tag="ACHY3M_004491"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4319265.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     ATLAAMPEMTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTV
                     ESARAVLAAGSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAM
                     P"
     gene            complement(8174..>8762)
                     /locus_tag="ACHY3M_004492"
     CDS             complement(8174..>8762)
                     /locus_tag="ACHY3M_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4319266.1"
                     /translation="LKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSY
                     SSARASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSA
                     WSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAE
                     RQKAGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT         1827 a         2799 c         2108 g         2028 t
ORIGIN      
        1 gcgcttttaa cctgcgaaaa actgtccgtc gcactttctg cccactcgcc ccagccggac
       61 ttcagaccgg ccatccagct tccacgaagc tgctcctccg cagaccaggt gttcttcagt
      121 gcagatgtgg ccttcgccag cgcatccgga ttatcaccgt acacgtcacg aaggcgctgc
      181 tcttccgact cccgctgcac ctgacggtcg gtgaggcccc gcgcctgggc actgattgcc
      241 gcctgcttcg cgccctgctg ctgctcaaac cgcgcagcct gctgtgccag ctcattcagg
      301 cgtttctggt attcaacctt gtcgcccagc tcagccagct ggcgtttgta ctccagcgtc
      361 tcgtctttat gcgccagcag ggatttttcc tgctcagata actgtcgttt cgtggcagcc
      421 tctttcagga ccgcatactg actttccgcc ttccataaat cgcgacgctg ctggctgatt
      481 ttcccattcg caccgctgtg tttttccagc gtccggagct cggtttcaag cgccagcagg
      541 gcagcatgcg cctggtcttc ctgacgctca ccggctgaca ctttgactcc tgacgacttc
      601 ggctttttca gcgtcgattc ataatccttt ttcgccgccg ccatcagcgt gttgtaatcc
      661 gcctgcagga ttttcccgtc tttcagggcc ttattcagtt cttcctgacg ggcggtatat
      721 ttctccagcg gcgtcagcag acgctcatac gccttctgcg cctctccggt atacttcagc
      781 tgtgatgcgt cccgttcggc ccggtccctg gcggccagtt caccggcttt ttccatatcc
      841 gactgcagcg tggccgctgc cagccccaga cgggcatttt cccggtcatt ccatgcgccc
      901 tgaaggttgg cccggaaaga ggaggtttta ccgcggcgct ggctccgact ctggtaccac
      961 tgccattttt tatccgcctc atcaaatgcc ttctgcgcac tggcgagcat atccgctgag
     1021 gattccggac gaccgatatc cagaatggca tcccacatcg atttgaatgc cttccctgtt
     1081 ttatccgccc aggtctccag tgttcccatg ttttctttca ggcgacgggt ctgctcatca
     1141 aagcctttcg tggcgatatc gttcgccgcc tgcaatgccc cggcctcgtc tccggaacgc
     1201 tgcagctgtg caacatacgc aatctgctct gccgtcacgt tacggaactg gcgcgccatc
     1261 gccatcagtc ccgacgtcgg gtcagtggtc agcttcccga aggcttcagc gactttatcc
     1321 acctccacac cggatgcaga cgcaaaacgc gcgacactct ggttgatggc atcaaactgt
     1381 tcaccaccac gcacaccggc attcaccagg gctgccagtg actctctcgc ctggttaaac
     1441 gtcagccctg ctgcctgccc ggctcttgag agagtcagca tacgatcggc agtcagtccg
     1501 gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atccccctgg
     1561 taccaggcgt acgccagcgc acctgtcgcc accgccagcg aggtgacccc gaccatcggc
     1621 agggtgatcg caccggcaag ccccctgaac atggggatca tcccgccgaa ggagtccttc
     1681 acctgaccgc cctgttgcag caggatcagc cagggattct gaccaccggc aagctgcgtg
     1741 gcgatatccg taaactgtgc gggcagggtt cgcatggccg ctttatactg cccgacggaa
     1801 atcccggctt tttgtgcagc cagcgcctgg cggctcaggc cctgttcaac agcactggcg
     1861 gtttttctgg cgtcggtatc cagacctgaa aaatgacgcc ttacccggct catctgctca
     1921 tcgaaacgga ccgcatccag actaaggtca ataacaagat caccaaccgg ctgggacata
     1981 tctcacacct ccggaaatcc ccgctgaagc catcattaat gcggcatcat ccaccatgac
     2041 atccgccaca tccgcagacg ataaaatatc gcgccctccg tccccaccga accggacgcc
     2101 tccggcaagt cctgccgctt tctgcatcag cattttgtcc tcatccggcc tctccacctg
     2161 ctcttcctca tgccggggga caagcagact gaaatcagag ggatgcatat ccggatcgca
     2221 aaaaaacagg ctgagtacag cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc
     2281 ctgaaaataa tgcgtgcggt aaaaacggtg ccagtcggca tattcggtgg atgtcatccc
     2341 ggcaagcatg gcgcgccagt cgggtctccc catctcacgc gccagtctga gggcaaagtt
     2401 cagctcgccg tcgaagactt tcccgcagaa aaatcatcat cagtcagcgt gttatttttc
     2461 gccacttcag taatatcagt atccggacga acagcttcga tcatcccgga caggcacaac
     2521 accacgtctt ccgcccgggc aatggcatcg gcaggccagg tggtgagcac ttcctgctct
     2581 atcttcatca cggcctcatt cattgacggt gactccgttt tctgtggatg gttatgccac
     2641 agggacatcg ccaccagaaa cgcgccggtt ctgacaaggt cttccacgct cacctgcagg
     2701 ttaccgctgg cttcagcctg ttctgcccgt cgtttcagca gggcaagatg ctcgatacgc
     2761 tgcagcgcag acagctcgga aagcgtgacg gacacaccgt tatattcaaa ttgttcggtt
     2821 ttcagaaaca tgtattacct ccgtttaccc tgcagcgccc gcttcagtaa cggtgacttc
     2881 agccactgcg gcgaactgac catttccgct caccacaggg atctgcacct tacctgtcgc
     2941 cacgccgttt accgtaattg tcatatcttt cacactaatg gtggctttcg acggatcggc
     3001 ggaaaccgct ctgaacgtct tgtctgttgc actttccggc tcaaaagaaa ccgtcagggt
     3061 ggttgttttc ccttttgcca ccgtaccgga tgtcggcgtc accttaatcg cactgaccgg
     3121 cgtaatttcg ctgcgttctt ccgccacaga aggtttaccc acgttggtca ctttcaccgt
     3181 acgggtgatc acttccttcg ccgttacggc cttaccgata ctgctgaccc agccacggaa
     3241 cacatccacc gtgccgttcg ggaaacggat tttataggcc cgcacatccc cgctttcaaa
     3301 ccagcctata agccctttct gaccttcttc tcccggtttc caggccagcg taaaactggt
     3361 atctcctgca gacttctgcc cctgcccggt cgcagtccag tccgcgtctt catcatccag
     3421 gtagttatca tcgtagggtt ctgccgtcat ctcgcccggc gtcagatcct ttacctgtgc
     3481 cagtcgctgc cagttatcgt ctgacaacgg gtttgcatag gcatcaccct gaccgttata
     3541 aacccacagg gtggtaccgg caccttttac cggtgccaga ggatttggtg ttggcatatc
     3601 gtcctcacat ctcgtatgta atggaataag tcaaatcagc agaactccat aacgccatat
     3661 cgtcatcacg acgatactca tagccctgcg taaccatcgt ggtaatcagg cctgacagtg
     3721 ccgggatcgc actcatcgcc ggataaatcc ggctttccat ccacgaatcc agctctgaat
     3781 ccggcacctg agcaggcagg aaaacttcaa tatgcagctc cgcctgccag gtatcgctgt
     3841 ccagcgcttc gcccgtgtat tcagcgccgg tgagataaac agcaattgcc ggaaaatccg
     3901 cctcatcaaa aacagcgggg cgaccatcaa acagcgtcgc cccgtgttca tgctgctcga
     3961 gtgcatccag cactgcagca cggatatcag tatgtttcat cgctttatca caatccttag
     4021 ttgttgtttc aacgcatagc ccagttcttt tggcagacgt tctcgccgga tacggttaac
     4081 gttctcatca aaagcctgct tcagtggggc cgccatcgga attttaacca cctgaatggg
     4141 aagacgatta cgctttttcc ttcctttatc gtcattgccc ttcgcatacc gggcttctgg
     4201 caaacgttgc ataacatgcc agcgcccatt atttaatcgc tggatgaatg cccgctgata
     4261 acgatgctga ccggctttaa gtatgctgtt cggacgatga ccaagcatcc tgatccccag
     4321 cttaatagca gggagatcac cgcggttaac gataattctg gcattcggat ttctgaccgt
     4381 cgcccgtttc agtctggacc gttcctttac cagtttccgg ctcaccctgg tctcccgggc
     4441 aacctgtgat gaagactgat taatcgccgt tgtagccacg cggttaatag ccattgctga
     4501 agccgacgga atggcgtttt tacgaacccg gctcagattt tcaatcgcct gatcaagccc
     4561 ttttatcgcc ataattcacc ctgcgtttat cgtcgccggt taactgcggg tggttgccca
     4621 cggttcagcc agagataaca actgcccccg tcatccggag aaacacgatc cacccagaac
     4681 gtctcaccat taatggtcaa cgtgtcacca cgccgcacgg cacgaaccgt atccgtccgc
     4741 acaaataatg acgggctgct tccttcaata cggatcccac tactggcaaa ccccagcgac
     4801 tccggatcgt caaaaacccc ctgaacttcg ccgccacgct gtgcaccgga ggtgaactgc
     4861 gcactgatgc ccatcacttc aacaatcgta ctgtccaccc cggcaagggc ggcatcaaag
     4921 gcattctgaa aatcacgcat aaacagccat tccaccatca acgtgtgttt ttgcatctga
     4981 ggacataatc agaatcaccc gaccaacatc cgcaagctca acggattccc ccgtttcacc
     5041 atcaacgcca cagagatgga ggcaggtcag aactctgatg cgcgttaacg cgccggatgt
     5101 ttcctcacga acatcatgag ccgcgttttc cctctcccgg atatccatat tcataacctg
     5161 tacatcatcg ccggatgact gcatttcctc ttcccattct gccacccgct gcgctatctc
     5221 tgcggcactc ccggatatat ccggctcacg ccccagaatc agggccagtt catcaagccg
     5281 tttcagattt tgctctttcg ttgccatatc atccccctgt gaaaaaagac acgggggcat
     5341 ttcgcccccg ctcacggatt atttcacctg taccaccaca aactcatccg ggtccggcaa
     5401 caccatcagc ggcgcggact gcgtcatggt aaattcacgg gcgggatccc ctaccgtcag
     5461 ccagtgtttc ggataacggg aagaggccac cacaccttcg gacaacgcct gcgcatcctg
     5521 aatggcacca tagcaacgaa tgccctctgc tgccgtattc cccaggacca gcgagccctc
     5581 aggcagataa cgtttttcgg taccgtcctc tgccacataa gacgtttttg ccaccacaat
     5641 ggccagatcg ccgtaatacc ctttgaagga caccaccgcg cccaggtctt tcaccgctgt
     5701 ttcgagttgc gaattagagc cgcgacgggt atccagtttt tcgcggaaca atttaaagcc
     5761 attcagcaga cgccagacgg taccgtccat aatggcaata ttcacaagac cgctggcctg
     5821 gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg tcacgatcct gttttgacca
     5881 ctccttacca ctaccctgag tgatgttatt cttcgtcgac ctgccaaaat cgacctcaat
     5941 tttctcgaac tggtctcctt ccatcgtgta tttgccatac agcacggcat tcaccgcctg
     6001 catttcttcc acctggacaa tggcgtgctc ttcctgtttg aggttatcag tgatgatacg
     6061 cagacgacgg taggccgggt cgttcagctg agccggatct tcaccgggaa gacgctcaac
     6121 cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag cccggacgta acacgcgggt
     6181 ttcaccacca cgatgacgca gcacttttcc ttcaacaacc ggggaaacat aggccgccac
     6241 cggcgttttt ccggtaattt tgtccagcat cacctcttcg gtatggaaat tcaccgtacg
     6301 gcggaaaaac agctccagaa acagcgcacg aaatttcact ttttgttcgg tataaccgag
     6361 taactggcgg gtcgtaaaca atcccataaa tcagttcctt tcattcagaa atcagtcagg
     6421 ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg tgactcaggg cactgccggc
     6481 aaaggcgttg gcctttttgt gttcatccac actttcaggc cagcggattg cctccgtcgc
     6541 aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg ccttcaagcg gcagtaccag
     6601 tatgccaacc gcactaccgg ctttttgtcc gtcccagacc accagtttcc cggtggcctc
     6661 atccagcatc aggggcgtca gtgccggtgt tgccgaggaa atcccgctgc tgcctgtggc
     6721 ggtatgagcc ggatcattac cggcaaaaat acgtatttcc gcacgctgtt cagtgatagt
     6781 tttcgtcacc atattgtaaa aacctcctgt tgatggtcag cactggcttc atggcatggc
     6841 catgagcatt ttcacgtccg catcaccgtc tgctgacgtc tgtggcacgc caccctgtac
     6901 cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg gcggttgtgg atgcagagac
     6961 cggttctgcc ttacctgatc ccgcagccag cacagcccgg gcgctctcca cagtcattcc
     7021 cgggcaggca gccagctgtt cagcctgcgc ctcagcccct tttgcctcat ccagtgccat
     7081 gatctgatca cggagtgagg gtccggcatc cgcctgcggt gaagcagcca ggatcgggcg
     7141 ggctttttcc accgtcatct ccggcatcgc cgccagcgtt gccgccagtt gttcacgtcc
     7201 tttcgcttcc tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc
     7261 cgttgcagtt aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat
     7321 cacactgatg gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga
     7381 ctgaccttca aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata
     7441 catcgctact ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg
     7501 gcgaacctct gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta
     7561 aatcagcgtg atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat
     7621 catcacgcca atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga
     7681 cgccagcagc atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg
     7741 ctgacggagg cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg
     7801 actgtcaatg tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca
     7861 ggcgacaatg ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg
     7921 caccagcgtg ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc
     7981 cggtcgttta cctccggcca tgtactcgtc cgtttcagcc agcattccgg gagcatcaaa
     8041 ctgtacctgc tgttgtggta ccgaaagact tgatgccccc atctcgcgcc cgagcgcgca
     8101 aaagaaaacc cgcgcatagg cgggctccag aagcagcggt tcattgaatg ctgcggcaat
     8161 aatgtgtgaa agattacgtc tcacgtggtg ttgtctcctc ttccggcctg cgactctccg
     8221 ctatctgctg ctcatacgcc tgcgctatcc acaccggacg tgagagtccg gctttttgtc
     8281 gctcagcaga ttccctgacc tgctggcgga aaatgtcctg ataatcctcg cccatcagcg
     8341 ccagctcttt ctcatacgtg ctcagtccgg cctcaatgcg catcactgat tcctggactt
     8401 ccttgagccc gtcaatggcc attcttccgg ctccaatcca ctcagcccgt gaccaggctg
     8461 atcgcgcctg ataaaaatca aaacgcgccc gtggcggacg aataatcccc cgaagaagtg
     8521 cctcttccag ccagcaggaa aacatctgcg tggccagccg ggacgcaata aattttcgtc
     8581 gtcccataaa atagcgccac gactcattgg cggaggcgcg ggcacttgaa taactgacct
     8641 tcgagtaatc ccgggacaac tgttcgtagg aaacgccaag accggcggcg atataccgca
     8701 gcagcgcctg ttcaagcgcc gaaaatccat tgtctgaatc ctgcgcagtc tgcagtttca
     8761 ga
//
LOCUS       DAZUFB010000151         8681 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZUFB010000151 DAZUFB010000000
VERSION     DAZUFB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.151"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(321..584)
                     /gene="rpsT"
                     /locus_tag="ACHY3M_004493"
     CDS             complement(321..584)
                     /gene="rpsT"
                     /locus_tag="ACHY3M_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4319267.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            687..906
                     /locus_tag="ACHY3M_004494"
                     /pseudo
     CDS             687..906
                     /locus_tag="ACHY3M_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
     gene            914..1855
                     /gene="ribF"
                     /locus_tag="ACHY3M_004495"
     CDS             914..1855
                     /gene="ribF"
                     /locus_tag="ACHY3M_004495"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFS4319268.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1898..4714
                     /gene="ileS"
                     /locus_tag="ACHY3M_004496"
     CDS             1898..4714
                     /gene="ileS"
                     /locus_tag="ACHY3M_004496"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4319269.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYMPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4714..5208
                     /gene="lspA"
                     /locus_tag="ACHY3M_004497"
     CDS             4714..5208
                     /gene="lspA"
                     /locus_tag="ACHY3M_004497"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4319270.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5296..5745
                     /gene="fkpB"
                     /locus_tag="ACHY3M_004498"
     CDS             5296..5745
                     /gene="fkpB"
                     /locus_tag="ACHY3M_004498"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4319271.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5747..6697
                     /gene="ispH"
                     /locus_tag="ACHY3M_004499"
                     /gene_synonym="lytB"
     CDS             5747..6697
                     /gene="ispH"
                     /locus_tag="ACHY3M_004499"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFS4319272.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6763..7677
                     /gene="rihC"
                     /locus_tag="ACHY3M_004500"
     CDS             6763..7677
                     /gene="rihC"
                     /locus_tag="ACHY3M_004500"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFS4319273.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7844..8665
                     /gene="dapB"
                     /locus_tag="ACHY3M_004501"
     CDS             7844..8665
                     /gene="dapB"
                     /locus_tag="ACHY3M_004501"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4319274.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1976 a         2155 c         2472 g         2078 t
ORIGIN      
        1 gaatggttaa agtgtatagg aatttaattc aaaaatagag tggtcttaag tttaatacct
       61 caatgtgtat cacaatatcc acattctttg taggggagtc tggagattga gtagatattc
      121 ttgttcagaa tgtatcagcc gatggttcta cgattcttaa gccacgaaga gttcagaaag
      181 tgtaacggca tgtctgcaaa agccagcatc ttttgactat ctggcaaccg gtagtgtgtt
      241 ctctcacgca tcataaaagc agcaggcata aaaaaacccg cttgcgcggg ctttttcaca
      301 aagcttcagc aaattggcga ttaagccagt ttgttgatct gtgcagtcag gttagcctta
      361 tgacgtgcag ctttgttttt gtggatcaga cctttagcag cctgacggtc cacgatcggt
      421 tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat agctgcgtat
      481 actttcttga tgaaagtacg catcatagag cgacggcttg cgttgtgctt acgagccttt
      541 tcagactgaa tggcgcgctt cttagctgat ttgatattag ccaaggtcca actcccaaat
      601 gtgttctata tggacaattc aaaggccgag gaatatgccc ttttagcctt cttttgtcaa
      661 tggatttgtg caaataagcg ccgttaatgt gccggcactc gttacgtagt gatggcgcag
      721 gattctacca gcttgcaggg tgtgaataca gcttttccgc gataaaaatt gcagcaggcg
      781 gtcactttct tcccgtgatt tgcgccatgg caatgaaaag ccacttcttt ctgatttcgg
      841 tactcaatcg ccggttaacc ttgaccgctg tactaaggta tactcggacg attttcactg
      901 ttttgagcca gacatgaagc tgatacgcgg catacataat ctcagccagg ccccgcaaga
      961 agggtgtgtg ctgactattg gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt
     1021 acagggcttg caggaagaag ggcgcaagcg caacttaccg gtgatggtga tgctttttga
     1081 acctcaacca ctggaactgt ttgctaccga taaagccccg gcacggctga cccggctgcg
     1141 ggaaaaactg cgttaccttg cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga
     1201 caggcgtttc gcggcgttaa ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt
     1261 gcgcgtaaaa tttcttgccg taggtgatga tttccgcttt ggcgctggtc gtgaaggcga
     1321 tttcttgtta ttacagaaag ctggcatgga atacggcttc gatatcacca gtacgcaaac
     1381 tttttgcgaa ggtggcgtgc gtatcagcag caccgctgtg cgtcaggcgc tggcggatga
     1441 caatctggct ctggcagaaa gtttactggg gcacccgttt gccatctccg ggcgtgtagt
     1501 ccacggtgat gaattagggc gcactatagg tttcccgacg gcgaatgtac cgctacgccg
     1561 tcaggtttcc ccggtgaaag gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc
     1621 gttacccggc gtggcaaaca ttggaacacg cccaacggtt gccggtattc gccagcagct
     1681 ggaagtgcat ttgttagatg ttgcaatgga cctttacggt cgccatatac aagtagtgct
     1741 gcgtaaaaaa atacgcaatg agcagcgatt tgcgtcgctg gacgaactga aagcgcagat
     1801 tgcgcgtgat gaattaaccg cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg
     1861 ttatgtaatc aaaccgaaac acggaaccga gaatctgatg agtgactata aatcaaccct
     1921 gaatttgccg gaaacagggt tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat
     1981 gctggcgcgt tggactgatg atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa
     2041 aaaaaccttc attctgcatg atggccctcc ttatgcgaat ggcagcattc atattggtca
     2101 ctcggttaac aagattctga aagacattat cgtgaagtcc aaagggcttt ccggttatga
     2161 ctcgccgtat gtgcctggct gggactgcca cggtctgccg atcgagctga aagtagagca
     2221 agaatacggt aagccgggtg agaaattcac cgccgccgag ttccgcgcca agtgccgcga
     2281 atacgcggcg acccaggttg acggtcaacg caaagacttt atccgtctgg gcgtgctggg
     2341 cgactggtcg cacccgtacc tgaccatgga cttcaaaact gaagccaaca tcatccgcgc
     2401 gctgggcaaa atcatcggca acggtcacct gcacaaaggc gcgaagccag ttcactggtg
     2461 cgttgactgc cgttctgcgc tggcggaagc ggaagttgag tattacgaca aaacttctcc
     2521 gtccatcgac gttgcttttc aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt
     2581 aagcaacgtt aacggcccaa tctcgctggt gatctggacc actacgccgt ggactctgcc
     2641 tgcgaaccgc gcaatctcta ttgcacctga tttcgactat gcgctggtgc agatcgacgg
     2701 tcaggccgtg attctggcga aagatctggt tgaaagcgta atgcagcgta tcggcgtgac
     2761 cgattacacc attctcggca cggtaaaagg tgcggagctt gagttgctgc gctttaccca
     2821 tccgtttatg ggcttcgacg ttccggcaat cctcggcgat cacgttaccc tggatgccgg
     2881 taccggtgcc gttcacaccg cgcctggcca cggcccggac gactatgtga tcggtcagaa
     2941 atacggcctg gaaaccgcta acccggttgg cccggacggc acttatctgc cgggcactta
     3001 tccgacgctg gatggcgtga acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca
     3061 ggaaaaaggc gcgctgctgc acgttgagaa aatgcagcac agctatccgt gctgctggcg
     3121 tcacaaaacg ccgatcatct tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa
     3181 aggtctgcgt gcgcagtcac tgaaagagat caaaggcgtg cagtggatcc cggactgggg
     3241 ccaggcgcgt atcgagtcga tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg
     3301 cacctggggc gtaccgatgt cactgttcgt gcacaaagac acggaagagc tgcatccgcg
     3361 tactctcgaa ctaatggaag aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg
     3421 gtgggatctt gatgcgaaag agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc
     3481 ggacacattg gatgtatggt ttgactccgg atctacccac tcttctgttg ttgacgtgcg
     3541 tccggaattt gccggtcacg cagcggacat gtatctggaa ggttctgacc agcaccgtgg
     3601 ttggttcatg tcttccctaa tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca
     3661 ggtactgacc cacggcttta ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg
     3721 caataccgtt tcgccgcagg atgtgatgaa caaactgggc gcggatattc tgcgtctgtg
     3781 ggtggcatca accgactaca ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc
     3841 tgccgatagc tatcgtcgta tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg
     3901 ttttgatcca gcaaaagata tggtgaaacc ggaagagatg gtggtactgg atcgctgggc
     3961 cgtaggttgt gcgaaagcgg cacaggaaga catcctcaag gcgtacgaag catacgattt
     4021 ccacgaagtg gtacagcgtc tgatgcgctt ctgctccgtt gagatgggtt ccttctacct
     4081 cgacatcatc aaagaccgtc agtacaccgc caaagcggac agcgtggctc gtcgtagctg
     4141 ccagactgcg ctgtatcaca tcgctgaagc gctggtgcgc tggatggcac caatcctctc
     4201 cttcaccgct gatgaagtgt ggggctatat gccgggcgaa cgtgaaaaat acgtctttac
     4261 cggtgagtgg tacgaaggcc tgtttggtct ggcagacagt gaagcgatga acgatgcgtt
     4321 ctgggacgag ctgttgaaag tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc
     4381 cgataagaaa gtgggtggct cgctggaagc ggcagtaacc ttgtatgcag aaccggaact
     4441 ggcggcgaaa ctgaccgcgc tgggcgatga attacgattt gtcctgttga cctccggcgc
     4501 taccgttgca gactataatg acgcacctgc tgatgctcag cagagcgaag tactcaaagg
     4561 gctgaaagtc gcgttgagta aagccgaagg tgagaagtgc ccacgctgct ggcactacac
     4621 ccaggatgtc ggcaaggtgg cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt
     4681 cgccggtgac ggtgaaaaac gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg
     4741 ctacgctggc tgtggctggt ggtagtcgtg ctgattatcg atctgggcag caaatacctg
     4801 atcctccaga actttgctct gggggatacg gtcccgctgt tcccgtcgct taatctgcat
     4861 tatgcgcgta actatggcgc ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt
     4921 tggttctttg ccggtattgc gattggtatt agcgtgatcc tggcagtgat gatgtatcgc
     4981 tcgaaggcca cgcagaagct aaacaatatc gcttatgcgc tgattattgg cggcgcgctg
     5041 ggcaacctgt tcgaccgcct gtggcacggc ttcgttgtcg atatgatcga cttctacgtc
     5101 ggcgactggc acttcgccac cttcaacctt gccgatactg ccatttgtgt cggtgcggca
     5161 ctgattgtgc tggaaggttt tttgccttct aaagcgaaaa aacaataata aaccctgccg
     5221 gataaggcct acagattgct gcgaaatcgt aggccgcatc cggcaaaaat cctcaaaata
     5281 taagagcaaa cctgcatgtc tgaatctgta cagagcaata gcgccgtcct ggtgcacttc
     5341 acgctgaaac tcgatgatgg caccaccgct gagtctaccc gcaacaacgg taaaccggcg
     5401 ctgttccgcc tgggcgatgc ttctctttct gaagggctgg agcaacacct gttggggctg
     5461 aaagtgggcg ataaaaccac cttctcgctg gagcccgatg cggcgtttgg cgtgccgtca
     5521 ccggacctga ttcagtactt ctcccgccgt gaatttatgg atgcaggcga gccagaaatt
     5581 ggcgcaatta tgctttttac cgcaatggat ggcagtgaga tgcctggcgt gatccgcgaa
     5641 attaacggcg actccattac cgttgatttc aaccatccgc tggccgggca gaccgttcat
     5701 tttgatattg aagtgctgga aatcgatccg gcactggagg cgtaacatgc agatcctgtt
     5761 ggccaacccg cgtggttttt gtgccggggt agaccgcgct atcagcattg ttgaaaacgc
     5821 gctggccatt tacggcgcac cgatatatgt ccgtcacgaa gtggtacata accgctatgt
     5881 ggtcgatagc ttgcgtgagc gtggggctat ctttattgag cagattagcg aagtaccgga
     5941 cggcgcgatc ctgattttct ccgcacacgg tgtttctcag gcggtacgta acgaagcaaa
     6001 aagtcgcgat ttgacggtat ttgacgcgac ctgtccgctg gtgaccaaag tgcatatgga
     6061 agtcgcccgc gccagtcgcc gtggcgaaga atctattctc atcggtcacg ccgggcaccc
     6121 ggaagtggaa gggacaatgg gccagtacag taacccggaa gggggaatgt atctggtcga
     6181 atcgccggac gatgtgtgga aactgacggt caaaaacgaa gagaagctct cctttatgac
     6241 ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc gacgcgctgc gtaaacgctt
     6301 cccgaaaatt gtcggtccgc gcaaagatga catctgctac gccacgacta accgtcagga
     6361 agcggtacgc gccctggcag aacaggcgga agttgtgttg gtggtcggtt cgaaaaactc
     6421 ctccaactcc aaccgtctgg cggagctggc ccagcgtatg ggcaaacgcg cgtttttgat
     6481 tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag gttaaatgcg tcggcgtgac
     6541 tgcgggcgca tcggctccgg atattctggt gcagaatgtg gtggcacgtt tgcagcagct
     6601 gggcggtggt gaagccattc cgctggaagg ccgtgaagaa aacattgttt tcgaagtgcc
     6661 gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc attagcagcc taagttatgc
     6721 gaaaatgccg gtcttgttac cggcattttt tatggagaaa acatgcgttt acctatcttc
     6781 ctcgatactg accccggcat tgacgatgcc gtcgccattg ccgccgcgat ttttgcaccc
     6841 gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg tctcggttga gaaaactacc
     6901 cgcaatgccc tgcaactgct gcatttctgg aatgcggaga ttccgctcgc ccaaggggcc
     6961 gctgtgccgc tggtacgcgc accgcgtgat gcggcatctg tgcacggcga atcgggaatg
     7021 gctggctacg actttgttga gcacaaccga aagccgctcg ggataccggc gtttctggcg
     7081 attcgggatg ccctgatgcg tgcaccagag cctgttaccc tggtggccat cggcccgtta
     7141 accaatattg cgctgttact ttcacaatgc ccggaatgca agccgtatat tcgccgtctg
     7201 gtgatcatgg gtggttctgc cggacgcggc aactgtacgc caaacgccga gtttaatatt
     7261 gctgccgatc cagaagctgc tgcctgtgtc ttccgcagtg gtattgaaat cgtcatgtgc
     7321 ggtttggatg tcaccaatca ggcaatatta actcctgact atctctctac actgccgcag
     7381 ttaaaccgta ccgggaaaat gcttcacgcc ctgtttagcc actaccgtag cggcagtatg
     7441 caaagcggct tgcgaatgca cgatctctgc gccatcgcct ggctggtgcg cccggacctg
     7501 ttcactctca aaccctgttt tgtggcagtg gaaactcagg gcgaatttac ctcaggcacg
     7561 acggtggttg atatcgacgg ttgcctgggc aagccagcca atgtacaggt ggcattggat
     7621 ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc tggctctggc gtcgtaacct
     7681 gtcacatgtt attggcatgc agtcattcat cgactcatgc ctttcactga tatccctccc
     7741 tgtttatcat taatttctaa ttatcagcgt ttttggctgg cggcgtagcg atgcgctggt
     7801 tactctgaaa acggtctatg caaattaaca aaagagaata gctatgcatg atgcaaacat
     7861 ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc cagttgattc aggcggcgct
     7921 ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc gaaggatctt ctttactggg
     7981 cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc gttaccgtgc aaagcagcct
     8041 cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt acccgtccgg aaggtacgct
     8101 gaaccatctc gctttttgtc gccagcatgg caaagggatg gtgatcggca ctacggggtt
     8161 tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc gatattgcga ttgtctttgc
     8221 cgccaatttt agcgttggcg ttaacgtcat gcttaagctg ctggagaaag cagccaaagt
     8281 gatgggtgac tacaccgata tcgaaattat tgaagcacat catagacata aagttgatgc
     8341 gccgtcaggc accgcactgg caatgggaga ggcgatcgcc cacgcccttg ataaagatct
     8401 gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt gaacgtgtgc ctggcaccat
     8461 tggttttgcc accgtgcgtg caggtgacat cgttggcgaa cataccgcga tgtttgccga
     8521 tattggcgag cgtctggaga tcacccataa ggcgtccagc cgcatgacat ttgctaacgg
     8581 cgcggtaaga tcggctttgt ggttgagtgg taaggaaagt ggtctttttg atatgcgaga
     8641 tgtgcttgat ctcaatagtt tgtaaccaca aaatatttgt t
//
LOCUS       DAZUFB010000152         8448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZUFB010000152 DAZUFB010000000
VERSION     DAZUFB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.152"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(177..383)
                     /locus_tag="ACHY3M_004502"
     CDS             complement(177..383)
                     /locus_tag="ACHY3M_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFS4319275.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            696..785
                     /gene="kdpF"
                     /locus_tag="ACHY3M_004503"
     CDS             696..785
                     /gene="kdpF"
                     /locus_tag="ACHY3M_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFS4319276.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            785..2458
                     /gene="kdpA"
                     /locus_tag="ACHY3M_004504"
     CDS             785..2458
                     /gene="kdpA"
                     /locus_tag="ACHY3M_004504"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFS4319277.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPDVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2481..4529
                     /gene="kdpB"
                     /locus_tag="ACHY3M_004505"
     CDS             2481..4529
                     /gene="kdpB"
                     /locus_tag="ACHY3M_004505"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFS4319278.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4538..5110
                     /gene="kdpC"
                     /locus_tag="ACHY3M_004506"
     CDS             4538..5110
                     /gene="kdpC"
                     /locus_tag="ACHY3M_004506"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFS4319279.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5103..7787
                     /gene="kdpD"
                     /locus_tag="ACHY3M_004507"
     CDS             5103..7787
                     /gene="kdpD"
                     /locus_tag="ACHY3M_004507"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFS4319280.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7784..>8448
                     /gene="kdpE"
                     /locus_tag="ACHY3M_004508"
     CDS             7784..>8448
                     /gene="kdpE"
                     /locus_tag="ACHY3M_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFS4319281.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYR"
BASE COUNT         1769 a         2184 c         2459 g         2036 t
ORIGIN      
        1 agccttaagt gagatatctg tcagataaga acaattagga aaatatttat atatatttca
       61 tgaatatatg tttcgattgg tataacgatg tctaaatatt cctctgaaca tcttcctgat
      121 acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt attcaaaact cagcaatcaa
      181 taaaaatcac cagttgcctt ttcggcctga tccatccaca ccggtttatc gctggttttc
      241 gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt ctttccagaa aagcatgaaa
      301 ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc gtaaaaagat aagccatgca
      361 ggatattctc tgtagagttc catacttttc tccccctttt gtatctaccc ggtgaatggt
      421 accggaaaaa tgaatttgtt tatctgatga aaatagtacc gccttttgtg taattttact
      481 actcatccga ccacttattt ttgcttattg atggtttatt tacattcatc ctgtaattaa
      541 gttacacaaa agttaaatta atactaaaca ttagttaaat catggctttt gccattttta
      601 tacttttttt acaccccgcc cgcagatttt tgcgaaatct ttgcagccag aattctaccc
      661 ttccggtatc acttttaggc cactggaggt gcactgtgag tgcaggcgtg ataaccggcg
      721 tattgctggt gtttttatta ctgggttatc tggtttatgc cctgatcaat gcggaggcgt
      781 tctgatggct gcgcaagggt tcttactgat cgccacgttt ttactggtgt taatggtgct
      841 ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat gacattcctc ttccaggtac
      901 agcgggcgtt gaacgtatac tctttcgttt acctgacgtc tctgaccatg agatgaactg
      961 gaagcaatat ctttgtgcca ttctcggcct gaacatgctg gggctggcgg tgctgttttt
     1021 tatgttgctc gatcagcact atctgccgct taatccacag cagttgccgg ggctgtcgtg
     1081 ggatctggcg ctgaatgccg ccgtcagctt tgtcacgaat accaactggc aatcttacag
     1141 cggtgaaacc acgttgagct atttcagcca gatggctggc ttaacggtgc aaaactttct
     1201 ttctgccgcc agcgggattg cggtgatttt tgccctcatc cgtgcgttta cccgccagag
     1261 catgagcaca ctcgggaatg cctgggtcga tctgctacgc atcacgttat gggtgctcgt
     1321 ccctgtggcg ttgttgatcg cactgttttt tattcaacaa ggtgcgctgc aaaactttca
     1381 gccttatcag gctgtgaata ccgttgaagg agcgcaacag ctgttaccca tggggcctgt
     1441 agcttctcag gaagcgatca agatgctcgg tactaacggc ggtggcttct ttaatgccaa
     1501 ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac ttcgtgcaga tgctggcgat
     1561 cttcttgatc ccaacggcgc tgtgctttgc ctttggtgaa gtgacgggcg atcgccgcca
     1621 ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc atctgcgtag gcgtggtgat
     1681 gtgggcagaa gttcagggta atcctcatct gctggcgctc ggcgcagaca gcagcatcaa
     1741 tatggaaggt aaagagagcc gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac
     1801 tacggcggct tcctgtggcg cggtgattgc gatgcatgat tcgtttaccg ctctcggtgg
     1861 catggtgccg atgtggctga tgcaaattgg tgaagtggtg ttcggcggtg tcggttctgg
     1921 tctttacggc atgatgctgt ttgtcctgct ggcggtgttt attgccgggc tgatgattgg
     1981 tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc gagatgaaac tgactgcact
     2041 ggcgattctg gttaccccga cgctggtgct gatgggcgcg gcgctggcaa tgatgaccga
     2101 cgccggacgt agcgccatgc tcaaccctgg cccgcatggt tttagcgaag tgctgtacgc
     2161 cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc ggattaagcg ccaactcccc
     2221 gttctggaac tgtttactgg cgttctgcat gtttgtcggt cgcttcgggg tgattatccc
     2281 ggtgatggca attgccggtt cgctggtgag taaaaagagc caacccgcca gctccggcac
     2341 gctgccaacg cacggcccgc tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg
     2401 cgcactgacc tttatccctg ccctggcgct tggtccggtg gcggaatatc tctcctgatg
     2461 atattgagtg agcactgaat atgagtcgta aacaactggc gctattcgaa ccaacacttg
     2521 tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc
     2581 cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac ctgtattagc atcgcgatgg
     2641 caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc aattagcggc tggctgtggg
     2701 tcaccgtact gttcgctaat ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg
     2761 ccaacagtct taaaggggtg aaaaaaactg cctttgcccg caagctgcgt gagccgaaat
     2821 atggcgctgc ggcggacaaa gttcctgccg atcaactgcg taaaggtgac atcgtgctgg
     2881 tagaagctgg cgatattatc ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg
     2941 atgaaagcgc catcaccggg gaatcggcac cggtgatccg tgaatccggc ggcgattttg
     3001 cctccgtcac cggcggcacg cgtattcttt ctgactggct ggtgattgag tgtagcgtta
     3061 accccggtga gacatttctg gatcggatga tcgcgatggt ggaaggcgca cagcgacgca
     3121 aaacaccgaa cgagattgcc ctgactatcc tgctgattgc tctgaccatc gtctttttac
     3181 tggcaaccgc cacgctgtgg ccgttttccg cgaggggcgg taatgccgtg agtgtaacgg
     3241 tactggtggc gctgctggtc tgtctgatcc caaccactat tggcggcctg ttgtcagcga
     3301 tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt gattgccacc agcggacgtg
     3361 cagttgaagc ggcaggtgac gttgacgttc tgctactgga taaaaccggc accatcacac
     3421 tcggtaaccg tcaggcgtcg gagtttatcc ctgcgcaggg cgtggatgaa aaaacgctgg
     3481 ctgacgccgc acaactggct tcgctggctg atgaaacgcc ggaaggccgc agtattgtga
     3541 tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt gcagtcgctc catgccacct
     3601 ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa catcgacaac cgcatgatcc
     3661 gtaaaggttc tgtcgatgcc attcgccgcc atgttgaggc taacggcggt cacttcccta
     3721 ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg ggccacgccg ctggtggtgg
     3781 tggaaggttc ccgtgtgctg ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta
     3841 aagagcgctt cgcccagctg cgcaaaatgg gcattaaaac ggtgatgatt accggcgata
     3901 accgtctgac tgccgccgcc attgctgcgg aagcgggcgt cgatgatttt ctcgccgaag
     3961 cgacaccgga ggccaagctg gcattgattc gtcagtatca ggcggaaggc cgtttggtgg
     4021 cgatgaccgg cgacggcacc aacgatgctc cagcgctggc gcaggcagat gtcgcggtgg
     4081 cgatgaactc cggtactcag gcggcgaaag aagcgggcaa tatggtcgat ctcgactcta
     4141 acccgaccaa gttgatcgag gtggtacata ttggcaaaca gatgctgatg acccgtggct
     4201 cgctgaccac cttcagcatt gccaacgatg tggcgaaata cttcgccatt attccggcgg
     4261 cgttcgcggc aacgtatccg cagttaaatg cgctgaacat catgcgcctt cattcgcccg
     4321 actccgccat tctcagtgcg gtcattttca acgccttgat tatcgtcttt ttgattcctc
     4381 tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc tgccatgttg cgccgtaact
     4441 tatggattta cggtctgggt gggctgctgg tgccgtttat tggtatcaaa gtcattgatt
     4501 tactgctgac cgtttgcggt ctggtgtgag gtttaccatg cgtggcttac gtccggcatt
     4561 atcgacattt atctttctgt tattgattac tggcggcgtt tacccgctgc tgaccaccgt
     4621 gctggggcaa tggtggtttc cctggcaggc caacggttcg ttgattcgtg aaggtgatac
     4681 ggtgcggggt tcggcattaa tcgggcagaa ttttaccggc aacggctatt ttcagggtcg
     4741 cccgtcggca acggcagaaa tgccctataa tccacaggct tctggcggga gcaatctggc
     4801 ggtcagtaac cctgagctgg ataaacaaat agccgcacgc gttgctgcat tacgggccgc
     4861 taacccggat gccagtacga acgttccggt tgaactggtg acggcatcgg caagcgggct
     4921 ggacaataat atcaccccgc aagcggcggt ctggcaaatc ccacgcgtgg cgaaagcgcg
     4981 taatctcagc gttgaacagc tcacgcaact gatcgcaaaa tacagccaac aaccgctggt
     5041 gaaatatatc ggccagccgg tcgtcaacat tgttgaactc aatctggcgc tggataaact
     5101 tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct gctggaacaa actgccgcgc
     5161 cgcatcgggg gaagctgaaa gttttcttcg gtgcctgtgc aggcgtcggg aagacctggg
     5221 cgatgctggc agaagcccag cgactgcggg cgcaagggct ggatattgtg gttggcgtgg
     5281 tagaaaccca tgggcggaaa gataccgccg ccatgcttga agggctggct gttctgccgc
     5341 ccaaacgcca ggcgtaccgt gggcggcata tcagcgagtt tgatctcgat gccgccctcg
     5401 cccgccgtcc ggcgctgatc ttaatggacg aactggcgca cagtaatgcg ccaggttctc
     5461 gtcatcctaa acgctggcag gatatcgaag aactgctgga agctggcatt gatgttttca
     5521 caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt ggtcagcggc gtcaccggaa
     5581 ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc cgccgacgac gtagtgctgg
     5641 tggacttgcc cccggacgat ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg
     5701 ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg caatctgatc gccctgcgtg
     5761 aactggcact gcgccgtact gccgatcgcg tagatgagca aatgcgcgcc tggcgggggc
     5821 atcctggtga agagaaagtg tggcacacgc gcgacgcgat ccttttatgc atcgggcata
     5881 acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt ggcctcgcgc ctgggtagcg
     5941 tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg cttaccggaa aaaaaacgtc
     6001 gggcaattct cagcgcctta cgtctggcgc aggagctggg cgcggagacg gctacactta
     6061 ctgatccagc ggaagaaaaa gcagtagtgc gttatgcccg tgaacataat ctcggcaaga
     6121 ttattctcgg tcgcccggcc tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac
     6181 tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc
     6241 gcacgattaa caacgcgccg gatagccgct cttttaaaga caagtggcgg gtacagattc
     6301 agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac tttaattgcc atgcagtggc
     6361 tgatggcgtt tgatgccgcc aacctggtga tgctgtatct gcttggcgtg gtggtggtgg
     6421 cgctatttta tggacgctgg ccttcggtgg ttgctaccgt cattaatgtg gtgagtttcg
     6481 atctcttttt tatcgcccca cgcggcacgc tcgctgtctc tgatgtgcaa tatctgctga
     6541 ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa ccttactgcg ggtgtgcgtt
     6601 atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg gcacttatat gaaatgtcga
     6661 aagctctggc ggtgggccgc agtccgcagg acatcgccgc caccagcgaa caatttattg
     6721 cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga tgacaacggt aaattgcagc
     6781 cgttaacaca tccgcaagga atgacgccat gggacgatgc catcgcgcag tggagttttg
     6841 ataaaggcct gcctgcgggt gcgggcaccg acacgttacc cggcgtgccg taccagattt
     6901 tgccgctaaa aagcggcgag aaaacctacg gactggtggt ggtggaaccg gggaatctgc
     6961 gtcagttgat gatcccggag cagcagcgcc tgctagagac gtttacgctg ttagtcgcca
     7021 atgcgtttga gcggctgacg ctgaccgcca gtgaagaaca ggcgcggatg gcaagcgaac
     7081 gtgaacagat ccgcaacgcc ctgctggcgg cgctttcgca tgatttacgc acgccgctta
     7141 cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcgcccc
     7201 acgcccgcca ggccagcgag attcgtcagc atgtgctgaa cactacccga ctggtgaata
     7261 atctactgga tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa
     7321 cgctggaaga agtagtcggc agcgcgctgc aaatgctgga accgggttta tcgtcgccca
     7381 tcaatctttc tctgccagaa ccgctgacct taatccacgt tgacgggcca ctctttgaac
     7441 gggtgctgat taatctgctg gagaacgcgg tgaaatatgc gggtgcgcag gccgaaattg
     7501 gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg
     7561 gtcttccgcc aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt
     7621 cggcagtacc gggggtaggg cttggactgg caatttgtcg ggcgatagtg gatgtacacg
     7681 ggggcactat taccgcgttc aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc
     7741 cccagcaaac cgctcctgaa cttgaagact ttcatgagga tatgtgacaa acgttctgat
     7801 tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacgggat
     7861 gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg gaagcggcta cccgtaagcc
     7921 agatttgatt attctcgatc tcggcctgcc cgatggtgat gggattgagt ttatccgcga
     7981 cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga
     8041 caaaatcgcc gcgctggatg ccggagcgga tgattatctg agtaagccgt ttggcattgg
     8101 cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac tccgccaccg ccgcgcccga
     8161 tccgctggtg aaattttccg atgttaccgt cgatttagcc gcccgcgtga ttcaccgggg
     8221 tgaggaagag gtgcatctca cgccgattga gttccgcctg ctggcggtgc tgctgaataa
     8281 tgccgggaaa gtgctcaccc agcgccagtt actcaatcag gtgtgggggc caaacgcggt
     8341 cgaacacagt cactatttgc gtatttatat gggacatctg cgacaaaaac tggaacagga
     8401 tcccgctcgc ccccgccatt tcattactga aaccggtatt gggtatcg
//
LOCUS       DAZUFB010000153         8354 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZUFB010000153 DAZUFB010000000
VERSION     DAZUFB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.153"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(140..427)
                     /locus_tag="ACHY3M_004509"
     CDS             complement(140..427)
                     /locus_tag="ACHY3M_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4319282.1"
                     /translation="MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSHVYNLASERMR"
     gene            complement(424..675)
                     /locus_tag="ACHY3M_004510"
     CDS             complement(424..675)
                     /locus_tag="ACHY3M_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4319283.1"
                     /translation="MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYC
                     VPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            complement(762..929)
                     /locus_tag="ACHY3M_004511"
     CDS             complement(762..929)
                     /locus_tag="ACHY3M_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319284.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLI
                     CRWRGIRITEE"
     gene            complement(1624..2496)
                     /gene="repA"
                     /locus_tag="ACHY3M_004512"
     CDS             complement(1624..2496)
                     /gene="repA"
                     /locus_tag="ACHY3M_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFS4319285.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            3199..3840
                     /locus_tag="ACHY3M_004513"
     CDS             3199..3840
                     /locus_tag="ACHY3M_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFS4319286.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            3981..4646
                     /locus_tag="ACHY3M_004514"
     CDS             3981..4646
                     /locus_tag="ACHY3M_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFS4319287.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            4913..5533
                     /locus_tag="ACHY3M_004515"
     CDS             4913..5533
                     /locus_tag="ACHY3M_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFS4319288.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            5560..7254
                     /locus_tag="ACHY3M_004516"
     CDS             5560..7254
                     /locus_tag="ACHY3M_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4319289.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            7335..7577
                     /locus_tag="ACHY3M_004517"
     CDS             7335..7577
                     /locus_tag="ACHY3M_004517"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFS4319290.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            7694..8029
                     /locus_tag="ACHY3M_004518"
     CDS             7694..8029
                     /locus_tag="ACHY3M_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319291.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            8186..>8354
                     /locus_tag="ACHY3M_004519"
     CDS             8186..>8354
                     /locus_tag="ACHY3M_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFS4319292.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLP"
BASE COUNT         2066 a         2198 c         2032 g         2058 t
ORIGIN      
        1 gactgcttga tgatgacttg tttctggaat caatgtctgt agcaaccctt tcgttttttc
       61 tttcacgaaa taaaccccca accttcccct gataaataag aacatctaac tcactaaaac
      121 ttaaaagcat ttacaccccc tatctcattc tctcgctggc gagattgtaa acatgactac
      181 gttcacgttt acctactgcg acaaccgcaa taattaacat gtcatcaatc acctgataga
      241 ccagacgaaa acctgatgcg cgaagtttta ttttgtagca atcttttaat cctcgtagtt
      301 ttgctgaagg tatatgagga ttttcgctgc atttttttag tttttttgcg aactgttgtt
      361 gaacggattt atccagtttc agccattccc tgagggcatc atccctgaat ttaaccgtat
      421 aagtcataaa taactatcca ggtccacatc atgtagtggc tgattgctgc gttcagtaac
      481 cagtttcacc agctcctggt catccatagc atcaagcatc ctttcataca attcagcagg
      541 aacacagtag aatgcaggct gattccggtt aagaatggca acaggatacc cctctccggc
      601 actgactgtc gccattggat tttttttcag ttcactgaca cttgcactgg tgtcagacaa
      661 aatgatactg ggcataacac acctctgaga ctgtttaaca ggtcttcacc ttacaccctg
      721 aaagactgct taacaagtct gtttgatgtt aatgtgtact gtcactcttc agttatcctg
      781 atgccgcgcc agcggcatat aaggcggcac tcagttgttt catatgatac cggagtaaaa
      841 ccgccgaagc ccggcgtcag ccggtactga ttgatagatt tcaccttacc catccccagc
      901 cctgctaagc catacccgct ttcagccatg agagagtttc tgtgcgcggt cggagtggtc
      961 ccgacgaggg tttacccgaa gtcggggcgt atctccgcgt tagcgggccg tcagggccgc
     1021 ttacgagcgt gtattcagta cgtccagcca gaagactaac agcgatgatg atgtttagtt
     1081 acaacattca taattaaaag cgatgttgtt cccgcccttc gggcgggggc ggcctcgctt
     1141 ttcagttatg agaggtgagc acggtgctgc gtttgtgtga tgtttttatt cctgatgtct
     1201 ttttgctgtg gtttcagaac ggtgaggttc ggggcccgcg cgttagcgtg ggaggtgcat
     1261 atttgcttgt ccacaggaca ggcactgaat aggttttctt ttcaaatgga agtaattaag
     1321 tagtttaaag gaaatacagt actggggttt aaaagaaaca ttgcaccctg taacgcctgc
     1381 ggctggcgct ttatagcatt aacggttgta accttacgga gaagtccctt gcagttaaat
     1441 gtggataaac agaattcccc gtctgtgaga cgtgttttgc gtgaaaaaca gggggagcaa
     1501 ggcgagccgg aaggcagatg atgaggctgt tttttgtgtg ctgtgctgct tttttgtgcg
     1561 tcgccgggct tcagacgtgc ggaagcctcc ggcgcaggcc ggattattca gatgaggtca
     1621 aactcagggt gaggcagtgg ccagccgtgt gtagttgttg ttccgcgaca ttatcatgcg
     1681 ttccttcacg cgacggtcga tttcgcgctt cacggcctcc aggcttccgc ggaaacgccc
     1741 ttccgcgatt tcacgtgtca gctgccgttt caccagggtg acaatgtcct gacgggtcct
     1801 gcctgcatcg cggcgcgcac gggcacgttt catgccgtgg gacttaagct ctgcctgata
     1861 actacggaaa cgctcgcgca caaaacgcca ggctctcgct atcagttcat ccatacccag
     1921 cgcatccagc ccctgctttt tgcgcagctt gttttcccat tccacgcggc tgcggcgtgc
     1981 ggaagccacc gcctcttctg atatatcgag tgaggaaaac agtgccggcg tgaacgtgat
     2041 gtcggtcggg atgttacagc caattgtcgg gtcgtattcc gtctggtagg ttatcagccc
     2101 cagctcagac aggaacttca gggcacgggt ggcacgggtg atggagagtt tcccggcctc
     2161 agactccgtc gccagtccgc actcaatggc cagcgtggtg atggagcact ggacgcggtt
     2221 ggccagcgga tcatagtgga aacacagccc ctgcagcagc gcatcaatcg cccggcggcg
     2281 cagcaccggc ggcatacgcc ggcgcaggcc gcgcgaacgg gcatacgcca catgcgcggc
     2341 aaaatcaaaa cgggaggtga agcccaccgc cttttccatc agtttttcgc agaacttcag
     2401 cgttccggca tcttcacggg gtgtgaacac cggattcggg ttctttacct gacggtatgt
     2461 gctttcagta aatgaggtct ggttcagggc cgccacaagc tgtcgcttat ggcggaaaca
     2521 gaatgccgtg cgttaataca ctgcgaaggg ttgaatctct gacacggttt catatatact
     2581 tatcccgtat tctgtgaggc ccccataatc ttctgtgcgt tccgccaagt tcgacaggaa
     2641 gattatgggg gttttccttt atggccttct gaaacttttt tcagccttcc ggccccggca
     2701 aactgccgtc gtttctgcca tacatcctga atgccttatg gcgcattctg ccgttttctg
     2761 gctttccgga ccgcgaggat cccaaaagcc actgaataac gatccgcaat ataccccttt
     2821 tccaccgctt tgaaaagccg acacgatttc tctgatttac caggcctctc attaaccacc
     2881 taaaaaacgt cgtttttttg aactatgtca aaaaaataga caattccaca aacgatcttt
     2941 tatgctgtgt ttttcatcat ttttagtaga agagatcgcc taaaattttt aaacgatcta
     3001 atcctccaga caccgcacac aaatcagcat aatatgctgc tttatcaaat ataacgatct
     3061 atttaactgc ttatcttcac tcttgccaca gatacatcct tatgtaatat atttgccagc
     3121 cattaatcct gtttgcagga gttgtcggtt ttcgcccgcc tcgaaaccgt ccacaaggct
     3181 tacaagagtg cgtaaatcat gtgcctgtca gaactgcagg ccgggctgaa cgagggtcag
     3241 ccgctgtttc cgacacatgc tttatgtatt cgccgttcac aacaacatcg aagcccctct
     3301 gcggggatcg attttcgcgg agcgttccgg attttgaata tcagtgaatt acatcaacgc
     3361 aactggtatc tggctcagta catccctgcc ggcaaaaacc gcgaacatct cttttcctgg
     3421 ctttccgaac aacacgtcct tccctggaca cccctgatac tgaaaaaggt ccgccgcacg
     3481 gataaagtct gtggttaccg tcgacatata cacgccgtgt ttcccggtta ctttttcctg
     3541 aaagcagacc ccgaacgcca cagcttcact caccttcgcc gtcacagtgc ctttctggac
     3601 ttcgtgaaaa tggccggtga aatcaaaacc gtcagggagg atatcgttca gtctttgatg
     3661 aaagtgtacc cggatcctgc gctcaatccg gcagccaggg aggagctgga tgctgcctcc
     3721 accctctggc tgacaaaagc gcgttaccag tacctcctcc ggcttgatgc acagcctctg
     3781 ccggagtcga ggatcgccct gctcctgcac ctggtatctg atgacggagc gttgacatga
     3841 aaattgctgt cttaatttga cgacttttcc cggcctttat ggccgggatt tttttccctt
     3901 ctgtttccac aatgcccggc accggtgaca cctgagggtg tacggagagc ggctgtcggg
     3961 cattttttca ggacgccttt atgaccattc tatcccagga aaaccctgtc attctgttac
     4021 tggagaatat gctcgaagca ctctccaccg ccccggacaa cataaacaac gaacggcgtc
     4081 gccggcgtta cctgctgaac tggcttgaca ctgcccgtca gatgcgcgag ttcagaggaa
     4141 tggctgaaga gttcactacc ctgcgaaaac tgctggccac caattcagac ataccggtag
     4201 caaacaccct gaaatcactg ctggaaagtg gcaataacgc agcattatac gacctgtacc
     4261 gcttccgctc ggctctttcc gcgtgcagaa gtctgggatg gcagattggc acgtgcgcat
     4321 ggaacgacca gctgctgagt gagacacttt cccgtgcgca aacaggtaaa cgtcatatcc
     4381 tgcagctgaa cagtaccaga gacacctttt ccaccacggg cagaatgctc agaccggtgg
     4441 cattttcatt aattcatccg gcatccctcg aatcttcaga agtggaagag atattccggg
     4501 atgaaggttt tatactcgcg cacggacgcg aatgcagcct gaacggcagc ggcagaaaca
     4561 tgctgtcacg cattcttcat gtggggcatt caggtttacc gaaagccgaa tggggcatag
     4621 atcacagcaa tcacctgtta cactgaaata ccgggacagc cacaacggca gccactctcc
     4681 gctgactttt tttcgcaaat cgcggtttgc tgatgtggat gtcccatttt ttattatccg
     4741 tcctctccgg ttgtcataaa gcgcattccg gcgtgaggct tctgttgtta tcatcccctc
     4801 ctgcagacat ccccttgtct tctgatgact gacgatatgc ttcttctgac aattaaagaa
     4861 gcggaagctt cacgccataa tgttgtttga ctgacctgcc acaggaggaa aaatgacact
     4921 gacgttactt accgcgtata acgcacccta tctggcagcc ctgacttttg ttctgcttac
     4981 tggtatcgct gaacttatcg ccctgctctg cggacactcc ctctcttcag ctatggatac
     5041 acctgattta ccggaggggc tgacaggaga ggcgctggac tggctgaaca tcggacggat
     5101 ccccctgctg atcgtcctct gtatgctggc aggttttttt ggtatatccg gcattctcct
     5161 gcagggtctt attattcatc tgctgcaggc tccagccccg aacattctcc ttgcaccact
     5221 ttgcctgctg ctgacatgtc ctctcgtaca ccgcaccggc agactgattg ccccctggtt
     5281 accccgtgat gaaacaacgg cgctcaccga ggaggctttc atcgggtgta tggccaccat
     5341 caccggttat cacgcagtgg cgggttcccc ctgtgaaggt cgcttcacag accgctttgg
     5401 gcaggttcat tacctgttgc tggagccgga ggccgggaaa gaattcagac gaggagacaa
     5461 agtgattatt gtctgccgtc tttccggttc ccgctggctg ggcgaactga atccctggcc
     5521 ggatgttctt taatcccgac atatacagag gttctcacta tgtttaatct tccgggatgg
     5581 ttgtttaccg ttattgcgct ggtcctcacc ctgttcgtta tcgggctgat atttgcccgc
     5641 ctgtaccgtc gggcatcggc agagcaggcc tttgtccgca caggtctggg aggccaaaaa
     5701 gtggtaatga gtggcggcgc aattgttatg cccatttttc acgaaacaat tcccgtcaac
     5761 atgaacaccc tgaagctgga agtcagtcgt gcagcggctg aaagtctcat tacccgcgac
     5821 cgcatgcgtg ttgatgttgc cgttgccttt tttcttcggg taaaaccatc agccgaagga
     5881 atatccactg cagcccagac gctgggacaa cgcacgctca cacctgaaga tctgcgtagt
     5941 ctggtcgagg ataaatttgt tgatgccctg cgggccacgg cggccagaat gagcatgcag
     6001 gatttacagg atgcccgcga gaactttgtt cagggtgtgc agaacaccgt tgcggaagac
     6061 ttatccaaaa acggtctgga gcttgaaagt gtgtcgctga caagtttcaa ccagactgca
     6121 cgggaacatt ttaatcctga caatgccttt gatgctgaag gcctgaccct tctgacccag
     6181 gaaacagaac gtcgtcgccg cgaacgtaac gaagttgagc aggatgttga agtggccatc
     6241 agggaaaaaa accgggatgc actctcccga cgtctggaga ttgaacagca ggaagccttc
     6301 atgactctgg agcaacaaca acgggtaaaa acccgtacgg cagagcagag tgccagtatt
     6361 gctgctattg aagctgaacg acgtcgtgaa gcagaatccg cgcgaattct ggctgaacgt
     6421 aagatagagg aagccgaaat cgaacgacag cagatagtgc gtacccgcca ggtcgaagcc
     6481 gaacgggaag tagccatacg ggaaattgag caacagcagg caaccgaaat cgccagccag
     6541 gccagagcca ttgccgtggc ggcaaaatcg gaagagcagt cacaggcaga agcccgggca
     6601 agtaaagcac tggctgaagc cgtacaggcc cagcaggatg tggaaaccac ccgccagact
     6661 gcggaggccg accgtaccaa acaggttgcc ctgattgctg ctgctcagga agctgaaacg
     6721 aaggcggttg aactgacggt tcgcgcccag gctgaaaaag aagcagccga actccaggca
     6781 gcagcaatta ttgaactggc ggaggccacc cgtcagaaag gtctggcaga agcggaagca
     6841 cagcgcgcac tgaacgatgc cattaacgtg ctttcggatg agcagaccag ccttcggttc
     6901 cgtcttgctc tgctggatgc attacctgcc gtcatagaga agtccacaga accaatgaaa
     6961 tccattgacg gcattaaaat cattcaggtg gatggcctga atggcggtgt tacccgcggt
     7021 aaaacagcaa caaccgacat aagtggcgga aatctcgctg aacaggcggt aaccgccgct
     7081 ctggcttacc gaacacaggc tccgctcatc gactccctgc tgaaggaggt ggggttatcc
     7141 ggtgaaggac ttacagccct gacacgtcca ctggtcatgc ctgccccgga aaaggttccg
     7201 gatgtcagcg aaccggtaac cgctccggat aactcagaac cacaacaggt ttaatccccg
     7261 tgccggaatt tttccggcat ctttttcaca aagaatgccc tcacgggata ttctggttta
     7321 aaagaggatt gaaaatgtcc gcagaaaaat tcagagttgt catatacacc aatacatgtg
     7381 aaaagaaagt cagttatatt cctgcaacag aagatataaa catcgctata atgccatttc
     7441 agacggcaga caatggctta attattgtgt gtcagaactc ccgtataata cgacgcaaag
     7501 agcgtatacc cggaagcagc tgtgaatgga aagatgtgac attagcacca aaatccagaa
     7561 accgcgttca gttatagcct gacagcctgc tttttatccc tcagaaaagg gggataaaaa
     7621 ttaaatattt cacaaccgag atatttttca ggaatgcaga ttattttatg ttatgaccaa
     7681 cagagaaaca ggcatgagaa caatcacaac gcgtgaacaa ttattaatta atgggagagt
     7741 ccacgaaaga atcgccacac atatagttac aggagctcat gggtatgaaa cgctttgcac
     7801 aaatggctat aacgtcagat acaacaaggc gcagcaactg gttgaaagct gcgaaaaaat
     7861 agctgaaggc aaactacctg ttacctgtcc tgtctgtttc gccatatggc aggatgtaca
     7921 caggttcacc cacgttgact ttgacacaca atcggggaaa agtgatttta ttgatacctg
     7981 ccattcggag atcataaccg gaaatgtttc aataacaggc aagaaataaa ataatgctcc
     8041 cgttctaaaa tgcaattcac tcttataatc cccccacgaa catgaacgtt cattatcaat
     8101 aacccgatga aatgatattc atctgaaggc cagttacccg ccacctgtct tataccgtaa
     8161 cccgtaatat accggatttt attctatgca aaaaaaatca cttacactcc ttctgccgtc
     8221 attactcagc ggctgtacgg catttaatgc cacgcctgtt cctgacacca cacctccggc
     8281 tctggtgttc gtcgacggac aaattcgcga aagcgctgcc attatcgccc atacgcaaaa
     8341 acgtctgctt ccgc
//
LOCUS       DAZUFB010000154         8336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZUFB010000154 DAZUFB010000000
VERSION     DAZUFB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.154"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..704)
                     /locus_tag="ACHY3M_004520"
                     /pseudo
     CDS             complement(1..704)
                     /locus_tag="ACHY3M_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1001..1603
                     /locus_tag="ACHY3M_004521"
     CDS             1001..1603
                     /locus_tag="ACHY3M_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFS4319293.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALTLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNNQVRQHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            1600..2202
                     /locus_tag="ACHY3M_004522"
     CDS             1600..2202
                     /locus_tag="ACHY3M_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS4319294.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            2214..5855
                     /gene="brxC"
                     /locus_tag="ACHY3M_004523"
     CDS             2214..5855
                     /gene="brxC"
                     /locus_tag="ACHY3M_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4319295.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNTDAMREVETWISMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKSKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAAES
                     EQTYVVVEKPVIQPVPKKTHLVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            5901..>8336
                     /gene="pglX"
                     /locus_tag="ACHY3M_004524"
     CDS             5901..>8336
                     /gene="pglX"
                     /locus_tag="ACHY3M_004524"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS4319296.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRARELGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLDHGFRMLSHPETPAAFEVLDHVPEVAEALLPESKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHAMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDDIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTDSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQKGDFQQKAAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGGKGMNGELKEFAKNNFPDSKADLFAMFMQHAFSLLKENGFNAQVNMQSWMFL
                     SSYEALRGWLLNNKTFITMAHLGARAFGQISGEVVQTTVWVVNNNHTEFYRPVFFRLI
                     NGSEEEKKSALISRKNMFNHTLQNDFKKIPGEPIAYWVSDIYRDIFTQSPALSSIAKP
                     RQGLATSDNNRFLKLWYEPSLRKINFSAKDRADALNSQAKWFPCQKGGSYRKWFGNHE
                     YLVNWENDGRELLDFAASLYGSPTRTIKNIPFYFREGATWSTISSSDFSIRYSPTGFI
                     SETKGAVCFADDKD"
BASE COUNT         2325 a         1937 c         1982 g         2092 t
ORIGIN      
        1 tattaagttc tttatcttta tatataccac gtatagtcct tctctttttt ctgactgcta
       61 atcagctgtt catcacgttt ttcccgaaat ttaacttgat attcttccaa ttcaggaacc
      121 ggtatgattt gctctgcatt gataagaaca tcatcattaa atctataagg cgtcagacga
      181 acgcatcgaa tgtctacccc cttatctctt aaccaaatcg cagtggtagt cagttccttg
      241 ctaaaatccg atgaagccaa aacaattctg atatcttttc cgaaatcaac aagctcagtt
      301 tcatccagct caacaaattc aagtattttt tctttcgcat cggcatcgca gttttgtttt
      361 ttcaaatacg tctggaaata ttcacatgct ttcgcaaaac tcattgtgga aatcatcgcc
      421 gcatatcgta gtgcctgtaa ctccatatgc gcacccgttt catcacgttt taattcgata
      481 accactaaat ttgcctgttt atcgattgcc aataaatcaa ttcgccgacg actatcttcc
      541 cagtcagaaa actcttcgga aattaccaga caatcaggag caataatatc tatgtttttt
      601 ttgattgctt cctgtaaatc ataacgttcc tgtaatcctt ccagagtaaa ggtcgttttt
      661 tctaaagcat gtagttcttt atttgtgata ttgtacagcg ccacgtttat cttccttttt
      721 taactaattt tacagggact ttaacacagt taaaatttta tttcctgagc tgtaatcgat
      781 taagctgatg cattcaatgg gaatgatata gggtaatttc cagtctcact catagaaatg
      841 gctaaagcat gactctcgcc aaaaccgttt atgtgttgta cataacgcga tcatccctct
      901 cacaaattgc cttttctcat ggcatctcgc ccagtccccc attacaatca ctttttgttt
      961 tttgcgagct gcattccagt cttcagaggg tttttcgatg attaaaaatg acaaggcatg
     1021 gataggagac ttgctgggcg ggccgctcat gagcagggaa agccgcgtca ttgccgagct
     1081 gttgctaacc gatcccgatg aacagacatg gcaagagcaa attgttggcc acaacatttt
     1141 acaagcctct tctcctaata ccgcaaaacg ttacgcggca acaatcaggc ttcgcctgaa
     1201 cacgcttgat aaaagcgcgt ggacattaat tgccgaaggt agcgagcggg aacgccagca
     1261 acttctgttt gtggctctga cgctacattc gccggtagtt aaggattttc tggctgaagt
     1321 ggtgaacgat ctgcgcaggc agttcaagga aaagttgcct ggcaatagtt ggaacgaatt
     1381 tgttaataac caggttcgcc aacatccggt actcgccagc tactcagatt catctattgc
     1441 aaaaatggga aacaatctgg tgaaggcgct tgctgaagcg ggttatgtgg atacgccccg
     1501 cagacgtaac ctgcaggcag tttacctttt accggaaact caggcagtgt tacagcgcct
     1561 ggggcaacag gacttgatat ctattctgga gggaaaacgg tgatagatcc cgttcttgaa
     1621 tatcgcctgt ctcaaatcca gagtcgcatt aacgaagatc gcttcctcaa aaataacggc
     1681 tccggaaatg aaattggttt ttggatcttt gattatcccg cacagtgcga actgcaggta
     1741 cgggagcatt tgaaatatct gctccggcat ctggaaaagg accataaatt tgcctgtctg
     1801 aatgtcttcc aaatcatcat cgatatgctc aatgaacgcg gccttttcga gcgcgtctgc
     1861 cagcaggaag tcaaagtggg tactgagacg ctgaaaaagc agcttgctgg tccgttaaat
     1921 cagaaaaaga tcgctgattt tatagcgaaa aaagtcgatc tggctgccca ggattttgtc
     1981 attcttaccg gcatgggcaa cgcctggcca ttagtacgcg gtcatgaact gatgagtgcc
     2041 ttgcaggatg tcatggggtt caccccactg ctgatgtttt atcctggcac ctacagcggg
     2101 tacaaccttt ccccgctcac agacaccggt tcacaaaatt attatcgcgc tttcagactg
     2161 gtaccagata cgggacccgc agcaacattg aatcctcaat gaagagcata acaatgaata
     2221 ttgaacagat ttttgaaaaa cctctaaaac gaaatataaa cggggtagtc aaagcagagc
     2281 aaaccgatga tgccagcgcg tacatcgagt tagatgaata tgtcatcacc cgcgaactgg
     2341 aaaaccatct tcgccatttc ttcgaatcct atgttcctgc cactggcccg gaaaggatcc
     2401 gtatggaaaa caagatcggc gtatgggttt caggcttctt cggttcaggt aaatcgcact
     2461 ttattaagat tctttcttat cttttatcta accgcaaagt tacacataac ggtacggaac
     2521 gtaatgctta ctccttcttt gaagataaaa tcaaagatgc attattcctt gccgatatta
     2581 acaaagcggt gcattacccg actgaagtca ttctgttcaa tattgattcg cgtgccaacg
     2641 tagatgacaa agaagatgcc attcttaaag tcttcctgaa agttttcaac gaacgcattg
     2701 gatactgcgc tgattttccg catattgccc atcttgagcg cgagctggat aaacgcggtc
     2761 agtatgaaac ctttaaagcc gcgtttgccg atatcaatgg ctcacgctgg gaagacgagc
     2821 gcgacgctta ctacttcatc agcgatgaca tggcacaagc attaagccag gccacgcagc
     2881 agagtcttga agcctcccgc caatgggtgg aacaactcga caaaaacttc ccgctggata
     2941 tcaataattt ttgccagtgg gtaaaagagt ggctggatga caatggtaag aacatcctct
     3001 ttatggtgga tgaagtcggt cagttcattg gcaaaaatac gcaaatgatg ctgaagctgc
     3061 agactattac tgaaaacctt ggggtaattt gcggtggccg cgcatgggtt atcgtgactt
     3121 cgcaggccga tatcaacgcg gcaatcggtg gtatgagcag tcgcgacgga caggacttct
     3181 ccaagatcca ggggcgcttt tcaacacgcc tgcaactttc cagctctaac acatcagaag
     3241 ttatccagaa acgtttgttg gtaaagactg acgaagcaaa agcagcgctg gcaaaagtgt
     3301 ggcaagagaa aggcgatatc ctgcgtaacc agctggcttt tgaccctaca acaactactg
     3361 cactacgtcc ttttaccagc gaagaagagt tcgttgacaa ctacccgttt gtcccgtggc
     3421 actatcagat tctgcaaaaa gtgtttgaat ctattcggac gaaaggtgca gcgggtaaac
     3481 aattggctat gggtgagcgt tctcagctgg aggcattcca gactgcggcg cagcaaatct
     3541 cagcgcaagg gctggattct ctggtgcctt tctggcgctt ctatgccgcc attgagagct
     3601 tcctggaacc tgccgttagc cgcaccatca cccaggcttg ccagaatggc attcttgatg
     3661 agttcgatgg caacctgctg aaaacgctgt tcctgatccg ctatgtggaa acgctgaaaa
     3721 gcaccctgga taacctggtc acattgtcta tcgataggat cgatgccgat aaagttgagt
     3781 tgcgccgccg ggtcgaaaaa agtctgaaca cgcttgaacg cctgatgctc attgcgcgcg
     3841 ttgaagataa atatgtgttc ctgaccaacg aagaaaaaga gatcgaaaac gagatccgta
     3901 acgttgatgt cgatttctct gcgatcaaca aaaaactggc atcgatcatc tttgatgaca
     3961 ttctgaaaag ccgtaaatat cgttatccgg ctaacaagca agactttgat atcagccgct
     4021 tcctgaacgg gcacccatta gacggcgcag tgcttaacga tctggtggtg aagatcctga
     4081 cccctaaaga tccgacctat tcgttctata acagcgatgc gacctgtcgc ccttatacgt
     4141 cagaaggcga cggctgtatt ttgattcgtc tgccggaaga gggccgtacc tggagcgata
     4201 ttgatttagt cgtccagact gaaaagttcc tcaaagataa cgccgggcaa cgtccggaac
     4261 aggcaaccct gctctcagaa aaagcgcgtg aaaacagcaa ccgggaaaaa ttactccgtg
     4321 ttcagttgga atcactactt gcagaagcag atgtctgggc gattggcgag cgcttaccga
     4381 aaaaatcctc cacgccatcg aatattgtcg atgaagcctg ccgttacgtg attgaaaaca
     4441 ccttcggcaa gctgaagatg ctgcggcctt ttaacggtga catctcccgt gaaattcatg
     4501 cattactgac ggttgagaac gacaccgaac tggatctcgg taacctcgaa gagtccaaca
     4561 ccgacgccat gcgcgaggta gaaacctgga tcagcatgaa tatcgaatac aataaaccgg
     4621 tgtatttacg cgatattctg aaccatttcg cgcgtcgccc ttatggctgg cccgaagacg
     4681 aagtgaaact gctggtagcc cgtctggcct gcaaaagtaa attcagcttc agccagcaaa
     4741 acaacaacgt cgagcgaaaa caggcgtggg agttatttaa taacagccgc cgccatagcg
     4801 aattgcgtct gcataaagtt cgccgtcatg atgaagcgca ggtgcgtaaa gccgcgcaaa
     4861 ccatggctga catcgctcag cagccgttta acgaacggga agagccggcg ctggttgaac
     4921 atattcgtca ggtatttgaa gagtggaagc aagagctgaa cgtattccgc gccaaggcag
     4981 agggcggaaa caatccgggg aaaaatgaga ttgaatccgg tctgcgcctg cttaatgcca
     5041 ttcttaatga aaaagaagat tttgccctga tcgaaaaagt ctcatcgctg aaagatgaac
     5101 ttctggattt cagtgaagac cgtgaagatt tggtcgactt ctaccgtaag caattcgcca
     5161 cctggcaaaa actgggtgct gcgctgaatg gcagctttaa atctaaccgc agcgcgctgg
     5221 aaaaagacgc cgcagcggtt aaagcgctgg gcgagctgga aagcatctgg caaatgccgg
     5281 aaccttataa gcatctcaat cgcatcacgc cgttgattga acaggtccag aacgtcaacc
     5341 atcagttagt cgaacagcac cgccagcacg ccctcgaacg cattgacgcc cgcattgagg
     5401 aaagccgtca acgcttgctg gaagcgcacg ccacgtcaga actgcaaaac agcgttctgc
     5461 tgccgatgca aaaagccaga aaacgcgctg aggtcagcca gtcgattccg gaaattttgg
     5521 cggaacagca agagacaaaa gcgctgcaaa tggatgcaga taaaaagatt aacctgtgga
     5581 tcgacgagct gcgtaaaaag caagaggcac aactccgggc agcaaatgaa gccaaacgcg
     5641 ctgccgagtc agaacagact tatgttgtgg tggaaaaacc cgttatccaa ccggtaccga
     5701 aaaaaacgca tctggtgaat gtcgccagtg agatgcgtaa tgccaccggt ggtgaggttc
     5761 tggaaaccac cgaacaggtg gaaaaggcgc tcgacacgtt acgcacaacg ctgctggccg
     5821 ccattaaagc aggcgatcgc attcgccttc agtaactccc atttcagggc agcactctgc
     5881 tgccctttgc aggattttct atgaatacca ataacatcaa aaaatatgcc ccacaggccc
     5941 gtaacgattt ccgcgatgcg gtgatccaga agctaacgac gcttgggatc gctgcagata
     6001 aaaaaggcaa tctgcagatt gccgaggccg aaaccattgg cgagaccgtg cgttacggtc
     6061 agtttgatta cccgttatcg acccttcccc gccgcgaacg gctggtaaaa cgcgcccgtg
     6121 agctgggttt tgaggtgctg gttgagcact gcgcctacac ctggtttaac cgcttatgtg
     6181 caattcgcta tatggagctg cacggttatc ttgatcacgg cttccgtatg ttgtctcacc
     6241 cggagacgcc ggccgcgttt gaggtgctgg atcatgtgcc ggaagtggca gaagccctgc
     6301 taccagaaag taaggcgcag ttggttgaaa tgaagctttc cggtaatcag gatgaagctc
     6361 tgtaccgtga actgctgctg gggcagtgcc acgccctgca ccacgcgatg ccgttcctgt
     6421 ttgaagcggt agatgacgaa gcggaactgc tgctgccgga taacctgacc cgtaccgact
     6481 ccattctgcg tgggctggtt gatgatattc cggaagaaga ctgggagcag gtagaggtta
     6541 tcggctggct gtatcagttc tatatttcgg aaaagaaaga tgctgtgatt ggcaaagtgg
     6601 tgaagagcga agatattcct gccgccaccc agctgtttac gccgaactgg attgtgcagt
     6661 atctggtaca gaactccgtt ggtcgccagt ggttgcaaac ctaccccgac tcgccgctga
     6721 aagacaaaat ggagtactac attgagcctg cggaacaaac gccggaagtg caggcgcagc
     6781 tggcggcgat taccccagcc agcattgaac ccgaaagcat taaagtgctc gacccggcct
     6841 gcggttccgg tcatattttg attgaagcct ataatgtgct gaaaaatatc tacgaagagc
     6901 gtggttatcg cgggcgtgat attccacaac tgattctgga aaataatatt tttggtcttg
     6961 atatcgacga ccgagcggca cagctttccg gctttgcatt attaatgatg gcacgtcagg
     7021 atgaccgcag aatatttacc cgcgatgtac gtctgaatat tgtctctttg caggaaagcc
     7081 tgcatctgga tatcgccaaa ctctggcagc agctcaattt ccaccagcag gtgcaaaccg
     7141 gcagtatggg ggatatgttt gctgaaaata ccgcgttaac ccaaactgac agcgcagaat
     7201 atcagctgct gatgcgcacg ttgaaacgct ttgtgaatgc aaaaacgctg ggctcactga
     7261 ttcaggtacc gcaggaagaa gaagcggaac tgaaggtatt tctggacgcg ctgtatcgcc
     7321 tggaacagaa aggcgacttc caacagaaag cggcagcgaa ggcgtttatt ccgtttattc
     7381 agcaagcgtg gattctggcg cagcgatatg atgcggtggt ggcaaatccg ccgtatatgg
     7441 gcggcaaagg gatgaatggc gagctgaaag agtttgccaa aaataatttc ccggatagta
     7501 aagctgattt gtttgcaatg tttatgcagc atgcattttc tttacttaaa gaaaatgggt
     7561 ttaatgcaca agtcaatatg caatcatgga tgttcttgtc gagctacgaa gcgcttcgcg
     7621 gttggttatt aaataataaa acatttatca caatggctca tttaggagct cgagcttttg
     7681 ggcaaatttc aggtgaggtt gtacagacaa ctgtatgggt agttaataat aaccatactg
     7741 agttttaccg ccccgtattt ttccgcttaa tcaatgggag tgaagaggaa aaaaaaagtg
     7801 cactaatttc gcgcaaaaat atgtttaatc acaccttaca aaacgacttc aaaaaaatcc
     7861 ctggagagcc aattgcttat tgggtaagtg atatatatag ggatattttc acccagtcac
     7921 cagctctctc ctcaatagct aaaccaaggc aagggcttgc aacttcagat aataatagat
     7981 ttttgaagct atggtatgaa ccatcattaa gaaaaattaa cttttccgca aaagatcgtg
     8041 ccgacgcatt aaattctcag gcaaaatggt ttccttgcca aaaaggtggg agttatagaa
     8101 aatggtttgg taatcatgag tatcttgtaa actgggaaaa tgatggccgt gaacttcttg
     8161 attttgcagc aagcctgtat ggttctccaa ccagaacaat aaaaaatatc cctttttatt
     8221 ttagagaagg tgcaacttgg tcgacaattt cgtctagtga tttttcaatt agatatagcc
     8281 caactggttt tatatcagaa accaaaggtg ctgtttgttt tgctgatgat aaagat
//
LOCUS       DAZUFB010000155         8271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZUFB010000155 DAZUFB010000000
VERSION     DAZUFB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.155"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(374..1567)
                     /gene="tyrB"
                     /locus_tag="ACHY3M_004525"
     CDS             complement(374..1567)
                     /gene="tyrB"
                     /locus_tag="ACHY3M_004525"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFS4319297.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1820..2899)
                     /gene="alr"
                     /locus_tag="ACHY3M_004526"
     CDS             complement(1820..2899)
                     /gene="alr"
                     /locus_tag="ACHY3M_004526"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4319298.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2952..4367)
                     /gene="dnaB"
                     /locus_tag="ACHY3M_004527"
     CDS             complement(2952..4367)
                     /gene="dnaB"
                     /locus_tag="ACHY3M_004527"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4319299.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4450..5433
                     /locus_tag="ACHY3M_004528"
     CDS             4450..5433
                     /locus_tag="ACHY3M_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFS4319300.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5599..5841)
                     /gene="pspG"
                     /locus_tag="ACHY3M_004529"
     CDS             complement(5599..5841)
                     /gene="pspG"
                     /locus_tag="ACHY3M_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFS4319301.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5975..7012)
                     /gene="dusA"
                     /locus_tag="ACHY3M_004530"
     CDS             complement(5975..7012)
                     /gene="dusA"
                     /locus_tag="ACHY3M_004530"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFS4319302.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7101..8199
                     /locus_tag="ACHY3M_004531"
                     /pseudo
     CDS             7101..8199
                     /locus_tag="ACHY3M_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2194 a         2111 c         2000 g         1966 t
ORIGIN      
        1 aacttacaca aagcggaaaa aaagagctct ctttaacttt agaatttaac gatactataa
       61 ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat atagaaatat
      121 tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat gtgcagaaat
      181 tattactcca cccaaaaaaa caggataagg aattccagat aattgggata agcttaacaa
      241 taaaagaact aacaacgttt attatctctc acgtagaacg atggcatcaa aaaaatcatc
      301 tataaagaaa gagtgaaagc gacgacagtt taatttcact gcaggctggg tagctccagc
      361 ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg
      421 tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac
      481 gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa
      541 gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc agttcctgac
      601 gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg
      661 cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt
      721 tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac
      781 acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat
      841 tgctcaccag agcgggtaat ccagcgctgg caatggcgcg aatagcgtag gcatcctctt
      901 ccataccggc accaaatcct tgataggcaa tatcgaggaa tggaataagc tcgcgggatt
      961 tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt
     1021 ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca
     1081 ggtcattaaa gcgcacgccg ttagtcgctt cgtcatacca ggggtaagta ctcacttcga
     1141 atccagcccc ggcgaatatt gctacgtggt tttcccaggt aggatcgctg acccagacgc
     1201 ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc
     1261 caagggtttg aatggttgct acgcgctgtt gttgcagtac tggatggtcc gcaccaaaca
     1321 gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag
     1381 cgccatgagg ctgcgcattc aggcgcgctt ccgcctccgc cacggcttgc agttgtggaa
     1441 taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt
     1501 cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt
     1561 gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag
     1621 aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactca
     1681 tgacaaacgt acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt
     1741 attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca
     1801 atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg
     1861 taataagttc gtaagcgctt actttcgtca tttcagcgat acgttctacg ggcaaacctt
     1921 cgccccataa aatgaccgga tccccggctt tgtcctgcgc ttgtggacct aagtctacgc
     1981 agatcatatc catcgccacg cgcccgacaa tcggtacttc gcgaccgttc accagcactg
     2041 gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa
     2101 gacgggtatc acgttcgctt acccaggttc caccataacc aacaggttct ccggctttat
     2161 gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg
     2221 caccggtgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc
     2281 agtcaaaatg cgactgtggc cacagcagaa tgccacccga tgcggcaatg gaacgttgac
     2341 caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt
     2401 ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc
     2461 actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca
     2521 taccggtatc gagtttcatc cagacggtaa ccggctcgtc caggctagcc tcttccagcg
     2581 cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca
     2641 gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac
     2701 gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc
     2761 gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac
     2821 tggcaggggc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca
     2881 caacagttgc cgcttgcatt tgtgctcctt gataagtgtt tgctttaatt acctaattca
     2941 taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat
     3001 tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata
     3061 atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac
     3121 atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccggg
     3181 cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg
     3241 ttcagttctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc cagcgtacgg
     3301 ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg
     3361 ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg
     3421 gaggagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg
     3481 cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac
     3541 agcgacgcca gagaacgcat cataatctgt tctgaaggca tctccagcga gaagataagt
     3601 actggtttat cctgcaacat cgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta
     3661 cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt
     3721 ttgttgagat cgtcataacc ggtgtttact ccggtaacgc catcgtgtgg ctgctgaaac
     3781 aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg
     3841 tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag gtccagcaga
     3901 tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt cgcaaccgag
     3961 atcatctcac ggacaacggc acgttcacgc acgatgtcag cataagcact gatgttcgcc
     4021 gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt
     4081 tgcccctggc gttccagcga ttccgcaagg gtaatcagat cgataggact accgctttcc
     4141 tgcaaacgcg ccatttcagt aaagatatgg cggtgtgggc gggtgtaaaa atcgtctgcc
     4201 accacacgct cggctacatc gtcccagcgt tcgttatcca gcattaaacc gcccaacacc
     4261 gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt
     4321 tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt
     4381 accgagataa agaatgggtc gaaactttac catatgaagc agcccctgac gatacgttct
     4441 ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa
     4501 gccgtagagt tcactcctgc cgatccggca gagaatgaaa tccaggtcga aaataaagcc
     4561 atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc gccatcacta
     4621 cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaag
     4681 catattaagg caggcgatcg tgttgtctat gcgcagtcgg cgttaggcgc ttacagctct
     4741 gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa
     4801 gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgtaa aacctatgaa
     4861 attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcgttgg cttgattgcc
     4921 tgccagtggg caaaagccct gggcgcgaaa cttatcggca ccgtaggaac cgcgcaaaaa
     4981 gcgcagagcg cgctaaaagc gggcgcgtgg caggtaatta actatcgtga agagaatctg
     5041 gtcgagcggt taaaagagat caccggcggt aagaaagtgc gcgtggtgta cgattccgtg
     5101 ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtcagt
     5161 tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcaaaaaggc
     5221 tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc
     5281 gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc
     5341 gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg
     5401 gcgacgcaag gttccagcct gctgattcca taaaagaaat agggcttcca cctgggaagc
     5461 cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc
     5521 aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt
     5581 gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt taggcacttt
     5641 tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag
     5701 caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag
     5761 cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat
     5821 cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca
     5881 agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag
     5941 ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga
     6001 gcgcgtgttc cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac
     6061 gccgccactg ccgcgcgcca ggaataccct ggaacaagcc caacatatgc cgggtaatat
     6121 ggccgagata cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta
     6181 ccgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct accgccgcca
     6241 gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaggt
     6301 gtgctttggc ctcttccagc gacttgatac caccgttaat cgacattgtc agatgcggaa
     6361 agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca cggttttctt
     6421 tcggacttaa ccccgaaagc caggctttac gtgcgtggat gataaacatc tcacactcgc
     6481 ctttgccgga aacggtgttg atgaaatcgc agagaaattc atagctgtcc tggtcatcga
     6541 tgccaatacg cgttttcacc gtcactggaa tcgacaccac atcgcgcatc gctttcacgc
     6601 agtcggcaac cagctgcgta ttacccatca gacacgcacc aaacatgccg ttctgcaccc
     6661 ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccacgcgct tctgccagct
     6721 tcgcacactg tgccagcgcc gccggatcgc taccgccgag ttgcaacgct accggatgtt
     6781 cttcttcact gtacgccagg taatcacctt taccgtgaat aatcgcccct gtggtcacca
     6841 tttcggtata cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt
     6901 ccgtccagtc gagcatagga gcaatgctaa accgagaatt ccagtaaaca ccagtttttt
     6961 caggcatcac gctggtttga ttaatttttt gtgcttcatg attatcgtgc atttttgaac
     7021 atttcaggct atttttctcg cgttaggttc ccgcacaggt tcccacgttt tatgggaacc
     7081 cgaaataacg aggtcgtgta atggcgtact ataacataga gaaacgacta aaatccgatg
     7141 gcacaccacg ctatcgctgt aatgtgatta tcaaagaaaa aggtgttatc acttacaggg
     7201 aaagcaaaac attccctaaa catgctcatg ccaaaacatg gggcacacag aaagtgatgg
     7261 aattagatct atatggcatt ccatcatcaa atgcagttga cggacttaca gtccgtgact
     7321 tactacacaa atatttaaat gacccaaatg ccggaggtaa agcaggccgt actaaaagat
     7381 atgtgctgga actgcttatg gatagtgaca tatccgcgat caaactatct gaactgacag
     7441 aaaatgacgt aattgaacat tgcaggctaa gaaacaacgc tggtgcaggc ccagcaacag
     7501 tcagccacga tgttagttat cttggcagtg ttctggatgc ggcaaaacct gtatacggaa
     7561 tcaattacac atcaaacccg gcgaaaagcg ctcgtccata tctacttaaa ctcggtttga
     7621 ttggtaaatc aaaccgtcgt aatcgtagac cagcatctga tgaacttgac atgctcattg
     7681 aaggccttca acaacgatct actcataaat gctcaaaaat tccgttcgtt gatatcctca
     7741 aattttctgt gtggtcctgt atgcgaatcg gagaagtatg ccggttacga tgggaagatc
     7801 tcgaccaaga acaaaaatct atactagtaa gagataggaa agatccacgt aaaaaggaag
     7861 gttgaccata tgaaagttgc cttgcttggg gaagcctggg atatcgtcca gcgacaacca
     7921 aaaaaatcag aattcatttt tccatataac agcacttctg ttaccgcagg atttcagagg
     7981 gtaagaagca aattaggtat taaagatctg cgatatcatg atttgcgtag agaaggggca
     8041 agtcgcttat ttgaggctgg ttttagtatt gaggaagtcg ctcaagttac agggcatcgt
     8101 tcattaaacg tgctatggca ggtatatacc gaactgtatc cgaaatcttt acataatcgt
     8161 tttgaagaac tccaaaagag cagaaacaag acctcttgac actgtttatt tatacagata
     8221 aaaataatac tgtatacaaa cacagtatag agggattttt atgcgtattg a
//
LOCUS       DAZUFB010000156         8227 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZUFB010000156 DAZUFB010000000
VERSION     DAZUFB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.156"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..970
                     /locus_tag="ACHY3M_004532"
     CDS             140..970
                     /locus_tag="ACHY3M_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4319303.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1002..1937
                     /locus_tag="ACHY3M_004533"
     CDS             1002..1937
                     /locus_tag="ACHY3M_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS4319304.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2030..2272
                     /gene="yhdT"
                     /locus_tag="ACHY3M_004534"
     CDS             2030..2272
                     /gene="yhdT"
                     /locus_tag="ACHY3M_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFS4319305.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2262..3713
                     /gene="panF"
                     /locus_tag="ACHY3M_004535"
     CDS             2262..3713
                     /gene="panF"
                     /locus_tag="ACHY3M_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFS4319306.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3725..4606
                     /gene="prmA"
                     /locus_tag="ACHY3M_004536"
     CDS             3725..4606
                     /gene="prmA"
                     /locus_tag="ACHY3M_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4319307.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4935..5900
                     /gene="dusB"
                     /locus_tag="ACHY3M_004537"
     CDS             4935..5900
                     /gene="dusB"
                     /locus_tag="ACHY3M_004537"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4319308.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5926..6222
                     /gene="fis"
                     /locus_tag="ACHY3M_004538"
     CDS             5926..6222
                     /gene="fis"
                     /locus_tag="ACHY3M_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFS4319309.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6308..7192
                     /gene="yhdJ"
                     /locus_tag="ACHY3M_004539"
     CDS             6308..7192
                     /gene="yhdJ"
                     /locus_tag="ACHY3M_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS4319310.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7276..7455
                     /gene="yhdU"
                     /locus_tag="ACHY3M_004540"
     CDS             7276..7455
                     /gene="yhdU"
                     /locus_tag="ACHY3M_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFS4319311.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7458..8120)
                     /gene="acrS"
                     /locus_tag="ACHY3M_004541"
     CDS             complement(7458..8120)
                     /gene="acrS"
                     /locus_tag="ACHY3M_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFS4319312.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2033 a         1979 c         2063 g         2152 t
ORIGIN      
        1 attcatttat ttctcaatga attaatctat ttttgttgcg atttttggcc catgattttc
       61 ttttcgaatt gtcgatatta ttgaaggata actttcaaag aaaaatacaa ggggctatag
      121 tgttacgcat taaaggccaa tgaagagtgt taacgtgccg agaaaatcgt ctaacctact
      181 gaaacgcaga ttaaaaattg cagaaattgt tgccgcccaa ggtgaaatca aagttgatga
      241 cttatctgcg caacttggtg tctctggcgt gacgatccgc ggcgatctca gttatctgga
      301 gcaacaggga tatttaaagc gatcatttgg cggtgctatt gcgacaccac tctcctctga
      361 accagaagcg atacaaccga tcgcgccatt gtctctggca caacaaatgg aaatagcacg
      421 acattgcgca cggttaattc gcgatcgtga cacgctattt cttggacatg ggacaatctg
      481 ccgtaagatc attccattgc tcagcgaagt gaaaaaactg cgtctgatca ccaacgatcc
      541 cgaacatgcg ctgctggcaa atcagtttat tgatggcgaa ataatactgg ctggcagtga
      601 aatgctgcgc cccgacaccg cactggccgg aaaaccgctt gaacaggcac ttcagcattt
      661 caccattaca catagcattc tggaaatcag ccatctggat gctgatggta ctttaaatat
      721 caacgttccc agactggccg ctgcctggca gctctgtttc gatcatgccc agaataagac
      781 ggttatcgtt gccgccgatc ctgcgccatc aacggctgct atcgggcatt tgcatcaggc
      841 agaaaatgtg atcgtcagcc gcagcattaa cgaacaatat cagcaacaat tgcagtcggc
      901 tgatttcgtt atcctttata ccagtaatga gtgctttacc tggtccaatc gcgaaaggct
      961 tcaggaatga gaggggtttt gtgacaataa ctatctgcgc tatgggcgaa ttgctggccg
     1021 agtttttgtc ccgcaaccca catcaaaaat tcactcagcc tggggagttt atcgggccat
     1081 ttcccagcgg tgcgccagca atttttgctg ctcaggtggc aaaactgtcc catcgggcca
     1141 tcttcttcgg atgtgttggt aatgatgatt ttggccgact cattatagag cgcctccgtc
     1201 atgaaggtgt cattaccgat gggatccatg ttatgaacaa tgccgtcaca ggtacggcgt
     1261 tcgtgagtta tcaaaatccc cagcagcgcg atttcgtctt taatatccct aacagcgcct
     1321 gcggcttgtt tactgctgag cacattgata aggatctgct aaaacagtgt aaccatctcc
     1381 atattgtggg ctcatcgttg ttctcatttc gcatgatcga tgtcatgcgt aaagcaataa
     1441 cgacgatcaa atcggctggc ggcaccgttt ctttcgatcc caatattcgc aaagagatgc
     1501 tgagcattcc tgaaatggcg caggctctcg attatttgat tgaatatacg gatattttca
     1561 tccccagcga aagcgaactc cctttcttcg cgcgtcacaa aaatctgtca gaagaacaga
     1621 ttgttagcga tcttctccac ggcggcgtaa aacatgtggc gataaaacgc gcccagcgtg
     1681 gggccagcta ttacaagctt aaaaacggta cattacacgc ccagcatgtt acaggccacg
     1741 atatcgaaat tatcgatcca acgggtgcag gcgactgctt tggcgcaacg tttatcactc
     1801 ttttcttatc cggtttcccg gcacataagg cactgcaata tgcaaatgcc agcggcgcgc
     1861 tcgccgtaat gcggcaaggt ccgatggaag ggatatcctc actggctgac attgaagact
     1921 ttttgcagca gcactgaacg gcaattttct gtcagattat tttccctgcc tgaagagttg
     1981 ccgtagaatt gcccgtttct cgtatccccc catgaggagg aacccttccg tggacactcg
     2041 ttttgttcag gcccataaag aggcgcgctg ggcgctgggg ctgacccttt tgtatctggc
     2101 agtttggtta gtagccgctt acttacctgg cgttgccccc ggttttaccg gctttccgcg
     2161 ctggtttgag atggcctgca tcctgacgcc gctgctgttt attggactgt gctgggcgat
     2221 ggtgaaattt atctatcgcg atatcccact ggaggatgac gatgcagctt gaagtaattc
     2281 taccgctggt cgcctatctg gtggtggtgt tcggtatctc ggtttatgcg atgcgtaaac
     2341 ggagcaccgg caccttcctt aatgagtatt tcctcggcag ccgctctatg ggcggtattg
     2401 tgctggcgat gacgctcacc gcgacctata tcagtgccag ttcgtttatc ggcgggccag
     2461 gagctgctta taaatacggg ctgggctggg tattgctggc gatgattcag cttcctgcag
     2521 tctggctttc actcggtatt ctcggcaaga agtttgcgat tcttgcgcgc cgctacaatg
     2581 cagtgacgct gaacgatatg ctgtttgccc gctaccagag tcgtcttctg gtgtggctgg
     2641 cgagtttgag tttgctggtt gcgttcgttg gtgcgatgac cgtgcagttt atcggcggtg
     2701 cgcgcctgct ggaaaccgcg gcgggtattc cttatgaaac cgggctgctg atttttggta
     2761 tcagcattgc gttatatacc gcctttggtg gctttcgcgc cagcgtgctg aacgacacca
     2821 tgcaagggct tgtgatgctg attggcaccg ttgtgctgct tattggcgtg gtacatgccg
     2881 ctggcggctt aagcaacgca gtacagacct tgcaaaccat cgatccgcaa ctggttacgc
     2941 cacaaggcgc tgacgatatt ctgtcgcctg cctttatgac gtcgttctgg gtactggtgt
     3001 gctttggcgt gattggcctg ccgcatactg cggtgcgctg tatctcttat aaagacagca
     3061 aagcagtaca ccgggggatc atcatcggta cgattgtagt cgcaatcctg atgttcggta
     3121 tgcacctggc cggagcgtta ggtcgtgcag tgatccccga tctcaccgta ccggatctgg
     3181 tgatcccgac gttaatggta aaagtgctgc caccgtttgc tgccggaatc ttcctggctg
     3241 cacctatggc tgcgatcatg tcgacgatca atgcccagtt gctgcaaagt tccgctacga
     3301 tcattaaaga tctctatctg aatatccgtc cggatcaaat gcaaaacgag acgcgtctga
     3361 agcggatgtc ggcggtaatt acgttagttc tcggcgcgtt gctgctgctt gccgcctgga
     3421 agccgccaga aatgatcatc tggctgaatt tgctggcctt cggtgggctg gaagccgttt
     3481 tcctgtggcc gctggtgctg ggtctttact gggaacgcgc caacgccaaa ggcgcgctaa
     3541 gtgcgatgat cgttggcggc gtgctgtatg ccgtactcgc gacgctgaat attcagtacc
     3601 tgggcttcca ccctattgtg ccctcgttac tactaagttt gctggctttc ctggtcggaa
     3661 accgtttcgg tacatccgtc ccgcaagcta ccgttttgac tactgataaa taaagagttt
     3721 taccatgcct tggatccaac tgaaactgaa caccaccggc gcgaacgcgg aagatcttag
     3781 cgatgcgctg atggaagcgg gtgccgtttc tatcactttt caggataccc acgatacgcc
     3841 agtatttgag ccgctgccgg gcgaaacgcg cctgtggggc gacaccgatg tgattggtct
     3901 gttcgacgct gaaaccgata tgaacgacgt ggtggcgatt ctggaaaacc atccgctgct
     3961 cggcgcaggc ttcgcgcata aaatcgaaca actagaagat aaagactggg agcgcgaatg
     4021 gatggataat ttccacccga tgcgctttgg tgaacgactg tggatctgcc ctagctggcg
     4081 tgatgtgccg gacgaaaacg ccgtcaacgt gatgttagat ccagggctgg cgtttggtac
     4141 gggtacccat ccaaccacct ctctgtgcct gcaatggctc gacagcctcg atttaaccgg
     4201 taaaacagtc atcgactttg gctgtggttc cggcattctg gcgatcgcgg cgctgaaact
     4261 gggtgcagca aaagccattg gtattgatat cgatccgcag gcgattcagg ccagccgcga
     4321 caacgccgaa cgtaatggcg tttctgaccg tctggaactc tacttaccga aagatcagcc
     4381 agaagaaatg aaagccgacg tggtggtcgc taacatcctt gcaggcccat tacgtgaact
     4441 ggcaccgtta atcagcgtcc tgccggtttc aggcggtttg ctgggccttt ccggtattct
     4501 ggcaagccag gcagagagcg tttgtgaagc ttatgccgat agcttcgcac tggacccggt
     4561 cgtggaaaaa gaagagtggt gccgtattac cggtcgtaag aattaacctt cgcatcgccg
     4621 tagggtgacg cggggcgaag tgcgagcaag ctcacaaaag gcacgtaaat ttgccggtta
     4681 tttacgcaaa tttgcgtgcc aaaattttca ttcataaaga aaaattgaga acttactcaa
     4741 atttctttga gtgtaaattt tagtcactat tttctaatat gatgattttt atgagtaatt
     4801 atcgcaccac gctcatttta aatgcaattc tttgatccat ctcagaggat tggtcaaagt
     4861 ttggcctttc atctcgtgca aaaaatgcgt aatatacgcc gccttgcagt cacagtatgg
     4921 tcatttctta actcatgcgc atcggacaat atcagctcag aaatcgcctg atcgcagcgc
     4981 ccatggctgg cattacagac agaccttttc ggacgttgtg ctacgagatg ggagccggat
     5041 tgacagtatc cgagatgatg tcttctaacc cacaggtttg ggaaagcgac aaatctcgtt
     5101 tacggatggt gcacattgat gaacccggta ttcgcaccgt gcaaattgct ggtagcgatc
     5161 cgaaagaaat ggcagatgca gcacgtatta acgtggaaag cggtgcccag attattgata
     5221 tcaatatggg ttgcccggct aaaaaagtga atcgcaagct cgcaggttca gccctcttgc
     5281 agtacccgga tgtcgttaaa tcgatcctta ccgaggtcgt caatgcagtg gacgttcctg
     5341 ttaccctgaa gattcgcacc ggctgggcac cggaacaccg taactgcgaa gagattgccc
     5401 aactggctga agactgtggc attcaggctc tgaccattca tggccgtaca cgcgcctgtt
     5461 tgttcaatgg agaagctgag tacgacagta ttcgggcagt taagcagaaa gtttccattc
     5521 cggttatcgc gaatggcgac attactgacc cgcttaaagc cagagctgtg ctcgactata
     5581 caggggcgga tgccctgatg ataggccgcg cagctcaggg aagaccctgg atctttcggg
     5641 aaatccagca ttatctggac actggggagt tgctgccccc gctgcctttg gcagaggtta
     5701 agcgcttgct ttgcgcgcac gttcgggaac tgcatgactt ttatggtccg gcaaaagggt
     5761 accgaattgc acgtaaacac gtttcctggt atctccagga acacgctcca aatgaccagt
     5821 ttcggcgcac attcaacgcc attgaggatg ccagcgaaca gctggaggcg ttggaggcat
     5881 acttcgaaaa ttttgcgtaa acagaaataa agagctgaca gaactatgtt cgaacaacgc
     5941 gtaaattctg acgtactgac cgtttctacc gttaactctc aggatcaggt aacccaaaaa
     6001 cccctgcgtg actcggttaa acaggcactg aagaactatt ttgctcaact gaatggtcag
     6061 gatgtgaatg acctctatga gctggtactg gctgaagtag aacagcccct gttggacatg
     6121 gtgatgcaat acacccgtgg taaccagacc cgtgctgcgc tgatgatggg catcaaccgt
     6181 ggtacgctgc gtaaaaaatt gaaaaaatac ggcatgaact aattcaggtt agctaaatgc
     6241 ttgattaaaa aggcgctact cggcatgggg aagcgccttt tttataggtg tcacaaaggg
     6301 agtgaccatg agaacaggat gtgaaccgac ccggtttggt aatgaagcta agaccattat
     6361 tcacggtgat gcgcttgccg aacttaaaaa gatccccgcc gaaagtgtcg atctgatctt
     6421 tgccgaccca ccatataaca tcggtaaaaa ttttgatggt ctgatcgaag cctggaaaga
     6481 agatctgttt atcgactggc tgtttgaagt gattgcagag tgccaccgcg ttctgaaaaa
     6541 gcagggcagc atgtacatca tgaacagcac ggaaaacatg ccctttatcg atctccagtg
     6601 ccgcaagctt tttaccatca aaagtcgcat cgtctggtca tatgacagtt ctggagtaca
     6661 ggcgaaaaaa cactacggct ccatgtacga acccatcctg atgatggtga aagacgcaaa
     6721 gaactacaca ttcaacggtg atgctattct ggtagaagcc aaaaccggat cgcagcgcgc
     6781 gttgatcgat tatcgcaaaa atcctccaca gccatacaat catcaaaaag taccgggtaa
     6841 cgtctgggat tttccgcgcg tgcgttattt gatggatgaa tatgagaacc acccgacgca
     6901 aaaaccggaa gccttactga aacgcattat tctcgcctct tccaacccag gcgatatcgt
     6961 tctcgacccg tttgccggta gctttactac cggtgccgta gccgtctcca gcgggcgaaa
     7021 attcattggt attgagatca acagcgagta catcaaaatg gggcttcgac ggctggatgt
     7081 cgcgtcgcat tactctgcgg aagaactggc gaaagtgaaa aaaagaaaga ccggcaacct
     7141 gtcaaaacga agccggttaa gcgaagttga ccccgatctc attgcaaagt aaaggaagtg
     7201 taagcctggt tttcagatta ttcatttcgt gtatatttcc tgccagactt ggttaaacat
     7261 acacaggctc aggtaatgat tcgcaagtat tggtggctcg tcgttttcgc tgtcttcgtt
     7321 tttctgtttg atactttact gatgcagtgg attgaactgc tggcaacaga aacagacaaa
     7381 tgccgcaata tgaactcagt taatccacta aaactggtta actgtgacga actgaatttt
     7441 caggacagaa tgtgaattta catgacactt aattcattcg tttgatgaat taatttcgtt
     7501 atgttttcat ctggcatgaa cattcttaat acgttatcga ccagagcggg ggcttgttta
     7561 taaagatcat aacccgccat attcattaac cagttttgaa caattccgct gaaggcacca
     7621 ttaataataa tcatcacaac atctaaatcg aggttatttg ctacacaacc ttgttgctga
     7681 cacgcctgca atacttcgcg gagagtctgc ggattgaagc ccatcttttc gcgtatcact
     7741 ccctcggcca gcatctcatc attaaattca catttgtgat ataagatttt cagcaacgcc
     7801 tgctggcggg gaattttggc aatatattgc aagccgacaa tcaatttttc acgcaattgt
     7861 tgaaacgggt catgctctaa tccagccgtc aagtgttcct ggattaactc ccgcaatgaa
     7921 ggctgttgca accacatctc attaaacagt tgagtcttgt tttcgaagtg ccagtagata
     7981 gcgccacgcg taacgttagc agcgtcggca atgtcgttga gtgtcgtctt gcttacgcca
     8041 tgctgcgcaa actgggcgat ggcagtttca atcagttctt gccgggtctt cagagcttcg
     8101 gctttggttc tttttgccat gattaattat tcaggaaata aatatattcg acacagagtg
     8161 agaaaatagc gaaggttaat ctatcaccta atgtgtattt atacgagagg ctaatattga
     8221 gttgcta
//
LOCUS       DAZUFB010000157         8158 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZUFB010000157 DAZUFB010000000
VERSION     DAZUFB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.157"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..168)
                     /locus_tag="ACHY3M_004542"
     CDS             complement(<1..168)
                     /locus_tag="ACHY3M_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319313.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKR"
     gene            246..1340
                     /gene="ddlA"
                     /locus_tag="ACHY3M_004543"
     CDS             246..1340
                     /gene="ddlA"
                     /locus_tag="ACHY3M_004543"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4319314.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1364..1576)
                     /gene="yaiZ"
                     /locus_tag="ACHY3M_004544"
     CDS             complement(1364..1576)
                     /gene="yaiZ"
                     /locus_tag="ACHY3M_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFS4319315.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1836..2144
                     /gene="yaiY"
                     /locus_tag="ACHY3M_004545"
     CDS             1836..2144
                     /gene="yaiY"
                     /locus_tag="ACHY3M_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFS4319316.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2203..3297)
                     /gene="yaiW"
                     /locus_tag="ACHY3M_004546"
     CDS             complement(2203..3297)
                     /gene="yaiW"
                     /locus_tag="ACHY3M_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFS4319317.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3310..4530)
                     /gene="sbmA"
                     /locus_tag="ACHY3M_004547"
     CDS             complement(3310..4530)
                     /gene="sbmA"
                     /locus_tag="ACHY3M_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFS4319318.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFGAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4882..6039
                     /gene="ampH"
                     /locus_tag="ACHY3M_004548"
     CDS             4882..6039
                     /gene="ampH"
                     /locus_tag="ACHY3M_004548"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFS4319319.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6040..6663)
                     /gene="iprA"
                     /locus_tag="ACHY3M_004549"
     CDS             complement(6040..6663)
                     /gene="iprA"
                     /locus_tag="ACHY3M_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFS4319320.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6751..>8158)
                     /locus_tag="ACHY3M_004550"
     CDS             complement(6751..>8158)
                     /locus_tag="ACHY3M_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4319321.1"
                     /translation="LEDSGFYGNSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVY
                     FSNSTLLGDVVFASTFNANFYPQGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSK
                     WVGSATTSANVDDESTASTDWYDVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTL
                     NNGSEWNTVNASNIDTLAVNNGSQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHL
                     TVDSYSTVALSSDDNSSIYNGYYTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLE
                     LTSGNIADHNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNSDAWGFGLKAGYDFK
                     LGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQA
                     LTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLG
                     GGDVDQDWSANVGVKYTW"
BASE COUNT         2127 a         2064 c         1916 g         2051 t
ORIGIN      
        1 tctcttcgtt aacttataat ttagagaaaa aaactcaccg catggccatt taagccagga
       61 ataaattagc ttcccgttca tcatttttgt gatcttgcgc acatctttga gataacgcac
      121 tttcttcacc agtacgttgc acgctttgtc agcagaagat tgcaacaatc tttcagaagg
      181 ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt gtgttagacg ggataggttt
      241 ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg taaatcagcg gaacatgaag
      301 tgtctctgca atcggcaaaa aacattgtcg atgccattga taaaagtcgc ttcgacgttg
      361 tgctgctggg cattgataaa caagggcaat ggcacgtcag cgatgccagc aattatctgc
      421 taaatgcaga cgatcctgcc catattgcgt tacgcccttc ggcgaccagc cttgcgcagg
      481 tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg
      541 tggatgtcat tttcccgatt gtccacggta cgctgggcga agatggttcc ttgcagggaa
      601 tgctgcgggt cgccaattta ccgtttgtag gttctgatgt tctggcttca gcagcctgta
      661 tggataaaga tgttaccaaa cgtctgctgc gcgatgccgg gctgaacatt gcgccattta
      721 ttaccctgac gcgcgccaat cgtcacaaca ttagttttgc cgaagtggag tctaaactgg
      781 ggttaccact gtttgtaaaa ccggctaatc agggctcttc tgttggtgtc agcaaagtaa
      841 ccagtgaaga acagtacgca attgccgtcg atctggcgtt cgaattcgat cataaagtga
      901 tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc agttctgggc aacgacaacc
      961 cgcaagccag cacctgtggc gagatcgtac tcaccagcga cttctatgcc tacgacacca
     1021 agtacattga cgaagatggc gcgaaagtgg tagttccggc agccattgcg ccagaaatca
     1081 acgataagat ccgggcgatt gccgttcagg cttatcaaac gttgggatgc gcaggcatgg
     1141 cgcgtgtaga cgtgttttta accccagaaa acgaagtggt gatcaacgag atcaacacac
     1201 tgcctggctt caccaacatc agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt
     1261 acaccgatct gatcacacgt ttgattgagc tggcgctgga gcgtcacgct gcggataacg
     1321 cattgaaaac cacaatgtaa tattttaacg cccgataata ttcttattgg gcgttttcag
     1381 tttcttcatc gcgacgacga ataatcagcc ccgccagcca gaaactaatc acccacgtca
     1441 ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa tcccagtaac cccaccacgc
     1501 cattaagaat gaatataagg ccaatggcga acgcatagta gtgccagtca cggcggattt
     1561 ttacaggcag attcatggca gctccgttaa cgaaaaacaa catccttgca tattctgtaa
     1621 caaccgtctg tggtgtagac aacattttcg taattgttaa taaatttaac ttaaggttgc
     1681 cattttgtga ctcacaacga agattcacgc ttcgggaata ttcccaacat caatttacca
     1741 tgattctgat attgacaatc taaatgacct gtcagacttg ccataagccg ctggtatcac
     1801 accagaagag aagcagatga cttccggagg tttttatggc tgatttcacc ctgtcaaaat
     1861 cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt
     1921 tgtttgtgct gttttgcttt tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc
     1981 ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg
     2041 gtttaggtgt cggcaccatt ttcgggctga tcccgttttt agtaggctgc ctcatttttg
     2101 cagtggtggc gctatggctg cactggcgac atcgccgcca gtaagattta cgttgccaga
     2161 tacggcctaa aacgccttat ctggcatgac attcctgttt gatcactgtt tcatacaacg
     2221 cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt
     2281 cgggctttcc agttgaatgc caggcaacat ttcgcggggt agtgatttac ccgttttcgc
     2341 ttcggcaagt tgataaactt tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc
     2401 tttctctaac tgacggcgaa tttcgctgtc gctcatcccc agttttcccg ccagtttgcg
     2461 cgttgccagt tcggttttcc cgggttcttt actgtcgtaa cgaattaaat caccgtccag
     2521 tgccagcttc acgccgctgg ccttactgac tgcgttctga aacgcggcat tacggctggc
     2581 gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt gcgctatagc tggcgggata
     2641 attcagtaaa tggtaagtac caaaccacaa cccgccgcgg cgactgaaga cttcctgacg
     2701 aactgtaccg tccattttcc acggataccc tttggtatgc tgttcggcaa aagcaatgct
     2761 gacctgcatc ggcccaccgg tgcgcacagg attaagcgaa ccaaacagcg tctgccccat
     2821 tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga
     2881 atctaaccgt tcgctataac ttttgccgtt tggcgatttg atttttagcg cggtatgaac
     2941 cagaaaagca ggaatgtgca tccgttcagc acgacggtca atctcttgcc aggcgatctt
     3001 gcttaagccc ggaacggccg gatctgcctg ataatttgac tcctgctgcg ccaccgccag
     3061 caccgagcag acgttttcca gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag
     3121 atctttcgcc caggcatcgc ggtctttcac gctcgcaggc atcttctgcc gcacgacgct
     3181 cgctacatct atcgcttttt cgcccttttt caacggttgc ggagcctgac tactacaccc
     3241 cgctaaaacc agaaccgcca acaatgacag cgaagataga tgattgacgc gcgacattac
     3301 ttctcctttt tagctcaagg tatgggttac ttcctgaatt ttgtcaccat ccagctcatg
     3361 ttcaaagctg cgcagacgtt tgtagataga catcaactca accagtgtgg tccatgagtt
     3421 aatcaggtac tggaaagcac cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag
     3481 gccgagcgta atcgtaccgg caacaattga cggaaacagc aagaacaaac cgaaaacgtt
     3541 atcgacctgc aaatagagaa tgcgggcgat gttgaaatac atatagtgaa aatagaggcg
     3601 aaaatagttt ttccgtacgg cgccaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt
     3661 ggcatcgtct tcaccataaa ccagctcttt acggtaggcg gcctctactc gctggttttt
     3721 aaactccagc cccggcagtt tgatccctac cactgccagc aatccggtcc ccatcagcga
     3781 ccagacgata gcggcaatca ccagaccata cggaatgtgc ccgataatcg gcagctccgg
     3841 cacatgcgcg gagagcgtta ccagcaccgg cagaaaggcg atcaacgtca tgatggcgtt
     3901 gataaaactg acgcccatat tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac
     3961 acgctgtgcg gcaccttcga tatgacgcag ttgttgccag ttcgccatgt aatattcgtt
     4021 catcgccgta cgccagcgga acacatagtg actgacaaag aagttgttca gtacactgat
     4081 caccaccgcg atcagcgcaa tccccagaaa gacgcccact tcgcggtaaa attgttcgat
     4141 agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga tcatagaacg gtgcatacca
     4201 ggcgttgacg gcgaccccga cttccaccaa aaaccaggtg acgaagatga tcagtgcagt
     4261 accgagtatt gaccagtatt gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa
     4321 aagtcctacg caaacaatgt agtaagcgta aaaaatcagg aaatccaacg accagaaacg
     4381 cgcggcgcta atcgggatct gcccggaagc gccggtgata cgcgccaccc agtcaccccc
     4441 accggcttgc cagaagataa cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt
     4501 tcccggcttt gggaaaaaag acttaaacat atgcactcct gctaacttct tatcgtttta
     4561 acaactgctg tgtattaacc gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca
     4621 ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc ataagaattg tttcgacgga
     4681 tttcacaaac caaagattgt ttacataagc ggattaacac gcgcaggatc acaaatgcag
     4741 gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt acgtttttaa cactattatt
     4801 gcactaattc gccctttgca atccatcaat gagtagtata aatacgctca gttaccttca
     4861 ttcaatctat ggacaccacc gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg
     4921 gcgtcattga cctccgtcca tgcggcgcag ccgatcactg aaccggagtt tgcctctgat
     4981 attgtcgatc gttatgccga tcatattttt tacggcagcg gtgccacggg gatggcgctg
     5041 gttgttatcg acggtaacca gcgcgtcttt cgcagttatg gcgaaacgcg acctggtaat
     5101 aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt ccctcaccaa gctaatgacc
     5161 agtgaaatgc tggtgaaatt gctcgaccag gggaccgtga agttaaacga tccgctaagt
     5221 aaatatgctc cgccaggcgc acgcgtgcca acctacaatg ggacgcccat tacgctggtc
     5281 aatctggcaa cccataccag cgcgctgccc cgtgaacagc ccggtggcgc ggcacatcgt
     5341 ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat acctttctac ggcgaagctg
     5401 aaagccgcgc caggcagtca ggcggcgtac tctaaccttg cgttcgacct gctggctgat
     5461 gcactggcga atgcgtccgg caagccttat acccagttgt ttgaagagca gattacccgt
     5521 ccattgggga tgaaagacac cacctacacc ccctcaccgg atcagtgccg ccgtttaatg
     5581 gttgcagagc gcggtgccag tccatgcaat aacacgctgg cggcaattgg cagcggcggc
     5641 gtctattcca cgcctggcga tatgatgcgc tggatgcagc agtatttgtc atctgatttt
     5701 tatcagcgca gtaaccaggc agatcgcatg cagacgttga tttatcagcg cgcgcagttt
     5761 acgaaagtga ttggcatgga tgtccccggc aaagcggatg cgcttggttt aggctgggta
     5821 tatatggtcc cgaaagaggg tcgtccgggg attattcaga agacaggagg tggtggcggt
     5881 ttcattacct atatggcgat gatcccgcag aagaatattg gcgcgtttgt ggttgtcact
     5941 cgttcgccgt tgacgcgctt taaaaatatg agcgatggca tcaatgacct ggtgaccgag
     6001 ctaagcggga ataaaccgct ggttatcccc gcgtcctgat taatactctg aaggcaaacg
     6061 gttgatagcg accagtttgc ctttattcat ttcgatatag ccgcctttac gcaaagcagc
     6121 gagaacttct gcgacgacag aacgcgatat gcgtgtacgt tgatggatat aattcatcac
     6181 accaatacgt gatcgcaatt cttcattcca gtcaatcatt gataataatg ttgcgcggat
     6241 ttgttcgtag gaattatgcc caatgagctg cacgtcgcgt aattccagaa ttctattttg
     6301 ccaggctaac cagtaaaaag cgtctcgcca gagttgattt tgttcaataa gcgtaatggt
     6361 ttgtttggct ggtagatgat atcccgtaca atttccttct gatattaatt tgtatggtat
     6421 atcgtttttc attaaaccat cagccagccc cataatataa ggagcctggg taataccgat
     6481 aagtacgttt tcttctctac gcagagagat aactccctct aaaataacga aagtatcttc
     6541 gttattgaca tcactggaaa atataacttg cttttcatta ttaaactcga agcgcgtacc
     6601 gtatctggac aaacatttat cgagcttacc aaattcctga agaggtttaa ctacagataa
     6661 catttgcgcg tcctttgcag taatgcccgt caaatccttg acgggcatta tttagattaa
     6721 attaccagaa tttcttcgga gtgaagaata ttaccaggta tatttaacac ccacgttcgc
     6781 ggaccagtct tgatctacgt caccaccacc gaggtagtta gcatcggtat aggcgctgaa
     6841 gttcttggtg aagctgaact gagtacccag accaacacgt accgcagacc cttcagtacc
     6901 gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc
     6961 cagtttgaag tacggagtca gagcctggtc ttcgctgtag gtgaaggtat aacctgcatc
     7021 tacacccagt tcataacgca tgctgtcgta agactgaccg tcaactttca tgtcgttgct
     7081 cagctggtag tcatcaccag actggaacag accagaaacg ctgccgtaag gagtcacgta
     7141 accagcatca cccagtttga agtcgtaacc ggctttcaaa ccgaagcccc aagcgtcgga
     7201 gttggacttg gaagtatccc aggtacggtc gttcaccaaa tcagcgttca gaacataatc
     7261 gctgctgtgg atatcgaata caccagacac tacgttgccg ttatggtcag cgatgttgcc
     7321 gctggtcagt tccaaagtat cagtctggaa cggattatca tcgaactggt ctacattaat
     7381 atccagtaaa ccaccgttag tcacagttat tgtatttgct aacagcgacg gagtgtaata
     7441 accattatag atacttgaat tgtcatcact agacagcgct acagtactgt agctatcaac
     7501 tgtcagatga tcagttccaa cataaccttc atctccaatg ttcaaagaag aaccattggt
     7561 cagagcgatg ctatcagaaa tcaagctaga ttcagacaca ttaacctgag agccattatt
     7621 cacagctaaa gtatcaatgt ttgaagcgtt aacggtattc cattcagaac cattattcag
     7681 agtgacgtta aacacaccgc tctggaatac ttgatcatca atcgtacgac cccaagcatt
     7741 tgcttctgtt acaacgcccg gatacaggct attacctgta acatcatacc agtcagtcga
     7801 agcagtagat tcatcatcaa catttgctga tgtagtagct gagcctaccc acttagaacc
     7861 attatccaga gtgatattca gttcatcaac attaagtgag tcatcagccc agccaccatt
     7921 ggtatccagt acgccatcgg cattactgtc atgaccttga ggatagaagt ttgcgttgaa
     7981 agtacttgcg aaaacaacat cacccagcag agtagaattg ctgaaataaa cattgttttt
     8041 catggagtaa tttgaagccg cactatcact gaaatacaga gctacatcat tagcttcgcc
     8101 gttaccagag taatcactag gttccgcaga gttaccatag aaaccactgt cttccaga
//
LOCUS       DAZUFB010000158         8122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZUFB010000158 DAZUFB010000000
VERSION     DAZUFB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.158"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..205)
                     /locus_tag="ACHY3M_004551"
     CDS             complement(62..205)
                     /locus_tag="ACHY3M_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFS4319322.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(267..533)
                     /locus_tag="ACHY3M_004552"
     CDS             complement(267..533)
                     /locus_tag="ACHY3M_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4319323.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFER
                     LTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
     gene            complement(553..810)
                     /locus_tag="ACHY3M_004553"
     CDS             complement(553..810)
                     /locus_tag="ACHY3M_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="HFS4319324.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
     gene            complement(820..1017)
                     /locus_tag="ACHY3M_004554"
     CDS             complement(820..1017)
                     /locus_tag="ACHY3M_004554"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="HFS4319325.1"
                     /translation="MVLTWFIFRIPSGPHKGRKIYEWDSQHGELEGYRASDGQHLGSF
                     DPKTGNQLKGPDPKRNIKKYL"
     gene            complement(1427..1570)
                     /locus_tag="ACHY3M_004555"
     CDS             complement(1427..1570)
                     /locus_tag="ACHY3M_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFS4319326.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(1632..1889)
                     /locus_tag="ACHY3M_004556"
     CDS             complement(1632..1889)
                     /locus_tag="ACHY3M_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4319327.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTE
                     HPSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
     gene            complement(1892..3637)
                     /gene="col"
                     /locus_tag="ACHY3M_004557"
     CDS             complement(1892..3637)
                     /gene="col"
                     /locus_tag="ACHY3M_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HFS4319328.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEV
                     SKDPELSKQFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNL
                     RITTPKRHIDIHRGQ"
     gene            complement(3870..6089)
                     /gene="mobQ"
                     /locus_tag="ACHY3M_004558"
     CDS             complement(3870..6089)
                     /gene="mobQ"
                     /locus_tag="ACHY3M_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFS4319329.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            6268..6543
                     /locus_tag="ACHY3M_004559"
     CDS             6268..6543
                     /locus_tag="ACHY3M_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFS4319330.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
     gene            complement(6761..7687)
                     /locus_tag="ACHY3M_004560"
     CDS             complement(6761..7687)
                     /locus_tag="ACHY3M_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4319331.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1957 a         2230 c         1806 g         2129 t
ORIGIN      
        1 aaaaaaaaag cgcatcagaa tcagcctgac acgctttgtt tttgtcaaag aggatttcgg
       61 gttactgcgt tgctaatccg gtcacttcag ctgttgatga cggagatacg gtcccgccct
      121 gaacatcccg gatatagttt gcctgacatg cagacagaat aatgactgca agaagcaata
      181 aaataatccc tgttattttt ttcataacga ctccttgttg tgaaagaatg ccagccattc
      241 atccataaac aaccggcatt catatttcag ccctgtttaa atcctgactt accgttagca
      301 gctcgccatt ctttaatttc cttgacaatc ccttcaggac tatcttccct gtcatcgcga
      361 ggataataaa tcagatctga accatctggg tgctcagtta atcgctcaaa ctctctcact
      421 aatttattgt catcctcttc agtagcacct tcagctctac atattttttt tacaagctcc
      481 agaaattcag cctcggtata atcactaata cttttcttta gctccacctt catctctcct
      541 gagaatattt gatcaccaat caccatcacg ataatcaaat gaaacaaaat actcattacc
      601 ggaaatatca atttgatgat taaagtatgg ttgtagcaaa ggtacccatt cagctataac
      661 atcaaaccaa ccattattga tattatcttt taaaggcatt ccaagacgtt caatgaccga
      721 gccatcatca ccgaaatctt ttgaatactc accaccttta aattcctcgg tcgtcttatc
      781 aaaccaatta atatgtaatt taagccccat aacatcctct caaagatact ttttgatatt
      841 tcgttttgga tcaggtcctt tcaactgatt accagtctta ggatcgaatg agccaagatg
      901 ctgaccatca ctggcacgat acccttcaag ttcaccgtgc tgggagtccc attcataaat
      961 cttacggcct ttatggggtc cgcttggaat tcggaaaata aaccacgtta agactatttt
     1021 ttagtcaaat aactttcagg aatcgccaga atggcgggac aacagcggtt ttggtgtccg
     1081 aaatcgtaca attctgtaca tctacgcaag gaaatgtacg gaagtaagcc aaatggttat
     1141 cggacggtcg gaacatttcg tacagccttt acgttcagtg agttagctgg cctagatgta
     1201 accactcgtt caactcaatt ctcttagcgt gggaaaattt ccgttttcca agcgcccccc
     1261 ccccgaatgg cgaacaggtc aaaatgacaa gttttctccc accggatgga aggttctgca
     1321 cagggatttt agatcccatc tcccacatgg aacttttgtg acatttcaaa aaaagcgcat
     1381 cagaatcagc ctgacacgct ttgtttttgt caaagaggat ttcgggttac tgcgttgcta
     1441 atccggtcac ttcagctgtt gatgacggag atacggtccc gccctgaaca tcccggatat
     1501 agtttgcctg acatgcagcc agaataatga ctgcaagaag caataaaata atccctgtta
     1561 tttttttcat aacgactcct tgttgtgaaa gaatgccagt cattcatcct taaacaactg
     1621 gcattcatat ttcagccctg tttaaatcct gacttaccgt tagcagctcg ccattcttta
     1681 atctctttaa taacagcttc agggctacca tcattattac cttctgggta ataaatcaga
     1741 tcagaaccac taggatgctc agtaacactt ataaaatgct cgaggttatc atcctgtttt
     1801 ttttcatcac cttcacaatt gatgatgtct tcaataattt ttttgaattc agtttcagtg
     1861 taatcactaa tgctgttttt tagttccatt tttactgacc tctgtgaata tcaatatggc
     1921 gtttcggcgt agtgatacgg aggttgtcca tatcataaac accaccatcc tgactgattg
     1981 gcttgtcgtg atgaagctca aatgagcgac gcccaccgac agtatcttta ttcctagccc
     2041 gtggagctaa tccttggctc aagcgttttt tattacctgg attaaattgc ttacttaact
     2101 caggatcttt cgacacttct tcccagaatt ttctccgaaa atcgtcgaag tttttaaatt
     2161 ctttatcacg caacttatca gcaatgcgat ctggaattgg cgctcctgaa tctttacctg
     2221 catcatccag ccatttatca ccaactggtt tacctttacc tgtcgccttc cctggcttat
     2281 tccgtttact ctccttatct aatttatcct tagcgtcctt ttctttattt tccttctgtt
     2341 tgcggcgctc ctgcgcggca cttaatgcag catcagcatc tgacttctct tttgcagcag
     2401 catcaaatgc agcctgctta ttatttacat ccgtctgcgc ccgctgggct ttaagcccgg
     2461 ccatttgcca cattctgtga ccgccagaca ttgggtcatg ggcaaatcga tcaaattgtt
     2521 ttatttcagc tattgcatca gcaagagtct tattcgctgc atcaagttcg cttttacgcg
     2581 aattataaac ctgaacagct ttagcctgtc gctcctgatt tctggcaaca tcttcatttg
     2641 cctgattcag ctctgcacgc gcgcgttcat aatttcgctc agccgcttca accggatgcg
     2701 tagcatccca ttcctgctga cggcgatttt cttcatcctg acgttgtttt acctggtcag
     2761 ggctaagaac atcactcact gaaacatata cggcattatg accgctgtcc ttcgggaatc
     2821 gaataactgc atccctggta ttaccaccct gagtaaatcc tgccgggcga acatccttat
     2881 cagtattatt tgtaacacct gggcttaatg tctgtactgc tggcgtactg ttattaactg
     2941 aaatattcag aacaggtgca cctggaattg atgccgtaaa aacacccgga cgttcggtag
     3001 gttttgcatc aaccaccgga acactcatcg gaacacctga aacaaccgaa atattctgtc
     3061 gttcgtcttt tacatcatca acaacacgaa catttacgtt tactgttgcc ttatcgagag
     3121 gtaatgaact gacaggtgat tcagtaatat catctgcggg taatgacgtc acaatctttg
     3181 acatcatatt ggggtcatct ttcgctattt gtgatggcaa tacaccatat aaagccaccc
     3241 cccaaagacc aaatttaaac ggtcctttca gggcagccat aatatcagca atagctgccg
     3301 ataatgctcc cgctgaaata ctgaccgcca gaccgccagc tcctggagtg gaaagtgccg
     3361 gaaaaccaaa tgccactggc acagctactg ctgacagatt accgcctgtt cccgagccac
     3421 caccggaatt accattcccc ccgccattac catgaccgga accaccaccc cagtgaatgc
     3481 cgctaccgga accaccaccc cacgggttat tttccgaact ccatccggag ccatcagaag
     3541 caccaccacc tacaccaagc ccggtcgggc caccattaat gttaccactt gtgctatgcg
     3601 cgcccgtgtt atggccgcgt ccatcgccac cgctcataaa aattccctct tttaagcgtt
     3661 aaaacaatta attacatgat taatactgta cataaaacca ctggttttat gtacagatca
     3721 aaaagtaccc gtcatcgttt tttctgtcaa gctcaggtgg aaaatttttt agaaatcagg
     3781 agggataaca cttagaaaac gcagagcggg ccacaaaaaa ttttgtggcc cgtgatgtgt
     3841 tggcggggtc cggggtgcag ccccggacat cacatactca tgccccggaa gtgcccgggg
     3901 cggcgttgtt gttgcctgtg ctgttcgtcc cgcttccact catgccaggt ctgattaaaa
     3961 ccgagttgac ggaagagata atccggcatc tcttccggtt tgtggacagc agccaaaagc
     4021 ctgtcatgat ggcctttgag ccggaactgc atcaggtgcg gttccggtgg gtacggagta
     4081 tcctcatcat ggtttatagc ttctccgtaa cctgccaggc ataccccgtt ctccgtgatg
     4141 gtgctgacta tcagatgact gtaatccggc gacggaagca caagaacggg gcaaaaccct
     4201 ccttcccggc gaacaaaccc gacaagcatt tcctccggcc ctttgcataa caacagattc
     4261 agcgtcctgc tgtttccctc tctcgaaaat acaacaggca tgagttgttc cggtacagca
     4321 tcagcggtga tatccacccg gtgcgctgac ggcagcggag aggctacggg cctgcggtcg
     4381 tcgctgaaat aaaaattatc cggccagtga tgcggcagcg ccggaaaacc gtaagagggc
     4441 cacccctggc gcatctgttc cagtacatgc cggaccgtcg caccggaata cattctcaca
     4501 cgctcaggcc gcgccatcgt ctcatgagat tctggcattc cctcacgtat atccgagacc
     4561 atcacagccc cgcctagcga caggtaaaca ttcaccgggt catgaacact ctgggggtaa
     4621 tgcgccagct catcacccca taccgtgaat tctccggctc tgttactcag catcatgtag
     4681 cgggttatcc tgcccgttcc cggctctctg gcacgcccca gctccgtaat aaccccgact
     4741 aatgccgttt cacggctcag acggtggtta tcatgacgtt tcagccgcgc ctcatgctcc
     4801 tgttccttcc ggcgcgcttc cgcttcggct tcctccctgc gccgctccgc ttcccgtgct
     4861 gacagttttt ctttcagctc tgccattccc ggcgcaatcc acgtattcca ctgcgtttca
     4921 aaggcctgac gacgggcggg aatgtcctgt tctttcacgg ccctgtaggt gcggcgaaga
     4981 gattcaatct gtttcccgag tttccagaaa cggagttgtc ttttgcggta ttccggttca
     5041 gcccatgtca tcaggggaat ctctgacaga agggacgcga cagggcgtgc ttttttgagt
     5101 ccggtcatct ccttttcgag aatggcctgc tctttcagca gcgcattccc ctcacgttcg
     5161 atgtcgttca ccttcccgct catcgccagg aactccgggg tcatctccca cgcgttgccg
     5221 cattcctgcg gcaacgtggc aatgaattcc cgcagggtat ccgctgaggc attcgcagcg
     5281 ggaatggtca ggttaagggc ttccgctgca cgacgtgcgg catcgctgag ttcccgtttc
     5341 cgcacgttct cctgtacctg gatgactgcc agccttgtgc gggaaacctg tatttccagg
     5401 ttaaggctgt taatcaggcg gttctgctca ccgcgttctg tttccattcc ccgtttttcc
     5461 atcgcacagg cagcctttcc gaggtgaagg cccggcgctt tctccagtcc ctgacgctcg
     5521 tatgaacgat ggtctatctc ttcctggtag ccggcgttcg caagcgctct gttcgcatgg
     5581 tcagcccatg atgcgcgcca cgtctcggcg agtgcccggt cgttccagtc cctgaccttt
     5641 cctgcgaatc ccgtttcccc gacagctctc gtggtcagca tgatgtgcgc atggggattg
     5701 gtccggtcca tgtgatggaa cgccacatcg gcaatcatgc cccgactgac aaagttttcc
     5761 cggacaaaag cgagaacggt ctcccgtgcg gcctcgcggg aaatctcacg gggaatggcg
     5821 agttcaatct cccttgccag ctgggagttc cggcgctgtt cggctttctc gaccgcattc
     5881 cagagaacgg aacggtcacc acaccaggac ggtgcattcg caggggtgag aatgaccgca
     5941 tcatcaacgc cgcgtttacg ggtgtaatcg tggattaatc cggtacggtc atcgggtatg
     6001 acagagccgg aacggtaggc agcagaagca acagcactgt agccgttttt tcgcgaaatg
     6061 attttcatgc tgagatgata aatcgccatt tctgccgtaa tgcctttttg ccgttttctt
     6121 ttagcgtcgc agaccgcaca caatcgaaac gaagtggaga ttgtataagt gcgcacttcg
     6181 tgatgtgccg tgcattatgc cagttcagtt ttgcaactac cagtgacgcg ataaaatgcg
     6241 atttttcacc gcgcacggag tcacaaaatg agcagaacac tcgaacagaa gattgccgaa
     6301 gctgaggcca gattacagcg cctgaaggcg aagagccgga gcctggacac ggcgcaaaaa
     6361 gtcattgtgg gggctgccat gctggcgagg gtacggaggc cggaagaggc gcaactgcgt
     6421 gcgttcctgc tccagtttct gcgaaaggaa gtcacaagac aggcggatgt gaaccgacta
     6481 caacctctga ttaacgagct ggagaagcta cccaaaccac ctgcgaaacc gcagaaccac
     6541 taagccccgg cgcgtaagag ccacatgcac caccggacgc ctttgcgctt cctcgctcat
     6601 tcgctcgcta cgctcggtca tgagactgcg gcgagcgcta caacctcaat catgcggaaa
     6661 gccgcgccat tgcgggattc aacgagggaa atagcaacaa accaagctgc tcaaaaaagc
     6721 gccaaaaagg cgctgttatc tgatataggt tttatttggt ctactctaca agacctcgtt
     6781 ttttcagttg gtaataccag gcgcggctaa ttccaagagt ctcccacggt ttcaacgttc
     6841 gcgcagatgt ggcaacagca ccacgcttag acttagcacc tccaattttc ccgcccttgc
     6901 gaccacgtgc agcctgaatt tctggcgtgt gcgtatcagc cacatactgt gcgaaagttt
     6961 ccggcgtgaa gttcctgtgc gtgtatttcg caatgctctt gccaatagcc cgacattcag
     7021 ccagtgaaag cggaacggga agcgatgcat tgtacatttc gacacgctgg atcaccgctt
     7081 caagccactg tgagaacgct ggccagccct gacgaatcgc cctgtaagcc catttgcgcg
     7141 tcatttcgaa caggtggcag tttcgcccca tcccgtaatg tttatcgatg ctacgacgct
     7201 ctgaggcgct caaatcgaga taatctgcca gttcatcgag ggtataggcg tcctcgcgcc
     7261 actccatcac ctgccattcc aggtggaacg gatttttgca aatcaggccg ctgtagttca
     7321 catcagcaca aagtttctca cacagcgaac gttcaaccgc tgcggcatat ttcagcgcct
     7381 taaccgaggc atcaggcgcg gttctcacgg cgatgttgag tgcgtagagc aggtgagcgt
     7441 gtccgttcac aggattttta acggtgatgt tcggcgcggg ggcgttccgg tcgctccagt
     7501 caatcgcagc cccctcgcgg tcaacgtcaa aaaccagcca gaactgcgca tgaggctggt
     7561 tctgctggat gtaccgcgca agaagcgcac gccctttgcc ggagatgcga aggccgaaag
     7621 cgagatcatc cgtgtgatag ggacgatggg gtaaattttc gtcgaagtat tgaagcgcgg
     7681 cgctcatgtg ttacgattct ctccgggtta tgcgacctac acgccctctg tatttcatgt
     7741 tgaatcttgc cggatctgga catgatttca ggggcgtttg tttttcagtc gcttacacta
     7801 cacccaacgg caaatcctgt cgatccttcc cgcaaaaccg atccatttac cataactccc
     7861 gttgtacgcc cccttaaaac gccctcagac gcgttctgac gcaaaaaacg caaaaagaca
     7921 ggaaaacacc aggcaaaacc gaaaaaacgc gtcagaatcg ttttcagggg cctgcataac
     7981 tattcacgaa agcgatgcgc gatcggtttc gtgcgttctg aacaccgaac ggctcaaaac
     8041 gacaagtttt ctccccctgg atggaaagtt ctgcacgagg attttagatc ccatctccca
     8101 catggaattt ttgtgacatt tc
//
LOCUS       DAZUFB010000159         8075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZUFB010000159 DAZUFB010000000
VERSION     DAZUFB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.159"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..880
                     /locus_tag="ACHY3M_004561"
                     /pseudo
     CDS             144..880
                     /locus_tag="ACHY3M_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1059..1356
                     /locus_tag="ACHY3M_004562"
                     /pseudo
     CDS             1059..1356
                     /locus_tag="ACHY3M_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1664..1780
                     /locus_tag="ACHY3M_004563"
     CDS             1664..1780
                     /locus_tag="ACHY3M_004563"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319332.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1999..3432
                     /locus_tag="ACHY3M_004564"
     CDS             1999..3432
                     /locus_tag="ACHY3M_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS4319333.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQTPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4253..4816
                     /locus_tag="ACHY3M_004565"
     CDS             4253..4816
                     /locus_tag="ACHY3M_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS4319334.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4971..7331
                     /locus_tag="ACHY3M_004566"
     CDS             4971..7331
                     /locus_tag="ACHY3M_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4319335.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7493..7669)
                     /locus_tag="ACHY3M_004567"
     CDS             complement(7493..7669)
                     /locus_tag="ACHY3M_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319336.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7807..8075)
                     /locus_tag="ACHY3M_004568"
                     /pseudo
     CDS             complement(7807..8075)
                     /locus_tag="ACHY3M_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2247 a         1595 c         1926 g         2307 t
ORIGIN      
        1 tttctgttaa ctggttttca tttttgaatc aatattttct gaacagggta atagtacacg
       61 tattaacaga ttatgtcaga aggccccggt gatgccttct gacgatagat taatgttgta
      121 ttctattggt atctggagac catatggcaa cagttacagt atattctccc cgctgtaatt
      181 cctataaagt gtaccgttat gatcacagca cctcagatca tgagcgtttc cagtgctaat
      241 cctgcaaacg tgtatttcaa ctgaattaca tctacgaaac ccgaaaacca ggcattaaag
      301 agcaaattgt tgaaatggtt cataacggag ccggggttcg cgatagctcc agaacgctga
      361 aggtggatat caacaccgtc attctcactt taaaaaacgc tcaccacgta aagtaaccat
      421 atctccagcc gccagtgtcg atgtggcact tatctgtgaa cttgatgaac aatggagttt
      481 tgtcgaaaac aaagctcgtc agcagtggca ctggtacgcg tataagacca aagctgacgg
      541 tgtgctggct tacacttttg gtcctcgcac tgatgaaaca tgccgtgagt tgccggaatt
      601 cctgaagcta ttctccgcag gcatgataac cagagacaac cggagcagtt ataccagaga
      661 aatgccacag gataagcatc ttgtcggcaa gatatttacc cggcgcattg agcgcaacaa
      721 tctgacactt cgtactcaca tcaaacgacc tgcccggaaa acgatttgct ttttacgctc
      781 cctggaaatt cacgaaaagc cattggttca tttatcgaaa acacatgtac tactgacagg
      841 agtcattacc agggcctctt ttgctgtttt tttgccatga atgataatga gaatcgacag
      901 cgataaggga aaagtaaaat aacactaatg ctgtcgtttt ctatcatatt gatttttaat
      961 tatttattgt cttttttctc aacccatttt tttacgcaga caaatttacc cggggaatac
     1021 aaactgtaac gacagttcac cacactatgg gggataaaat gccagatcaa tccggacact
     1081 gcaggactcc ttcacgcatc ttacggatgc ctgaactgag ccaattgtta ggtatatctc
     1141 gttcgacaat ttacgaaaaa atgaatccgt tatcgaaata ctacgatgca accttccccc
     1201 aggccggtac gtcttggtag tggttctgtg ggctggcgtt catcagctat tgacgaatgg
     1261 cttactttac atactgtacc tgccagatca gcagtcaaag gtgttaatga cgaagtgaga
     1321 catacgcttt cttcagatat gaccatgagc agataagttt ttgtcatcac gtgatcacct
     1381 caggaggaaa aagaagcata agaacaattt agccagaagt ttaattgata aacagaattt
     1441 ccagcattca ttgttgaaag agcagctatt gtcgctattg tagcggtaat tgctgctata
     1501 accgaaacgt attacgaaaa aggaagcatt tatgccttac atcaaaccgg atatgtcgta
     1561 ttcagtatat gcatgaccat caatgatagg gaaggcaata attaaagctc aatacaataa
     1621 acaagtacct ctgactctgg ttgcaatgtc agtattaaca gcgatgtcaa ttgcctgtca
     1681 gaatcaggtt gacgtatgca gtcccggaaa tttacgtggg gccgttaata tttacactat
     1741 ggttctggag aaagggaaaa gaccggggga aaggtattag tgaaataatt tccggaggta
     1801 ttaagaatga gaaatatgaa tgatatttcg tggtgaataa tacagtcttc actgaattaa
     1861 ccttatcggt ttattaaaca tcagatctac aaatgctctg tatttctcag agcatcaggt
     1921 ctgaatagca gctattttcg ctattgtagc gggatttgct gctattttac taacccatta
     1981 atgaaaagag aggcttttat gtgcttactt gcaccagaaa atccttatcc gatatatgca
     2041 ctaccaccgc tggtgagaaa tgcaataatt gaaactcaaa aaaatacaca gacacctttg
     2101 gctatggtgg caacatccgc attaacagcg atctcaattg cctgtcagaa tcagattgac
     2161 gtgtgcagac ctggaaattt acatgggcct gttaatcttt actctctgat tctggctgat
     2221 tccggtgaaa ggaaaacgac cgtggataaa gtgtttatga aagcatttta tctcagggat
     2281 gaagccctgg cggatgaata cgcgaaactg gttgagaatt acagtacaga aaaggaaata
     2341 tgggagcaaa aacaaaaagc gctggaatca aaatttcata aagagattcg tgccggtaaa
     2401 gattacaagg caacagaatc agagcttgaa acgcatctga ataaacctcc tgttccgccg
     2461 cagatacgtc gaacaatttt taatgagaca acgatagagg gaatgttaaa atattactcc
     2521 gatagcaatc gctcttttgc tcttgtatcc agtgaagggg gggtaatttt tgacagccgg
     2581 gccatgagta aactgggaat tattaacact ttgtgggatg gaggttctct tttcatcgac
     2641 aggaaatcat ctcccggaat taatttgaag gagccaagac tgacgatatc ggtgatgatt
     2701 cagcctgatg tttaccacaa aggtttttgt acgcgaaaaa aagaaattgt gaaaacgtca
     2761 ggacatcatg caaggttttt gatgtgtcaa ccaacatcaa cgcaggggac aaggataata
     2821 accggagata attattcatc acagtatcag gatttgtttg aagagaggat caatgagctt
     2881 attgatgaaa gtctggcgat gagtggtgaa cgacgttgcc ttcacttttc tcctcaggct
     2941 gccagaatct ggacggatta ttacaatgat gtggaatcga agctgggggg attggggcct
     3001 ttaagacatt gccgggaata tgctgctaaa aatgcagagt atatggcaag actggcagga
     3061 cttatttacc attcgagcgg tgaagagggg gagatttccc cttacactgc agaaatggcg
     3121 agagaattag caatatggta cggtaatgag tatgtgcggt tgtctaatcc gttaactttt
     3181 gacaactctg ccctgaccgt acctgtgcga ctcattccag aggagcttga acttttcaac
     3241 tggataaaaa gctattgtat tgagaagggg atcctctgta tgaaaaaaaa tgacatttta
     3301 cagcgtggtc cgaaccgttt ccggaaaaag gataaaatca actggttact ggatttactg
     3361 tatgaacaaa acagagttgt accggttatt gagggaaaaa cgttgtgtgt cgcacctaac
     3421 tttgacctct gaacgactta atcgttgagt acatatagtc tcctctgcta cttgcggagg
     3481 agactaatgt ctttaccatg tcagcgttgt attagaaaac tctggtacag gtcagtcagt
     3541 aaatataact ggtgtaacca ctatttacta ttggatagag tgttgctcag gatattgtcg
     3601 gctttagtct gaggtgaggt ggggccttca ttgttttcgt gttcaataaa taatttaggt
     3661 taattagtat tattagtgga agttatgtga tctgttatgt tgtcttgtac gcacttaaca
     3721 ctggttacgt accggaacac tattagagta tgttatggct ctgttacagt ttataggtat
     3781 gaaaaaatat aatctttcct gtatgaaata atattcttat ggtgtgtggg gtatggtctg
     3841 tataccatca tttttatggt gaagttaaat atctgtagag gccttaagct gggtcgctgt
     3901 ttttacttaa tggtacagaa gtattaatag cggtgttgcc tgtggctacc cgatactggc
     3961 ttttgtgaat gtcagattac ttcctgtaag tgattgaatg gtacttcctt aaactcaatt
     4021 ctatgaggtg tgtttttacc attgtaattc gtactggtcc tcagtttaag tgagtcttac
     4081 tgtactctga aatattacct ctttggagta atgaggtaat acgaaacagt tttatcaggt
     4141 tgtctgtgct gctggttacc tcgtttatta atgccgcggt acggcgtttc tgtactctca
     4201 gtatttatgt aattccacct tcctgtaact tttataaagt aaggactata aaatgtataa
     4261 tgctaatccc aattacgaaa tggacttcat gattctgaaa gacgtgaatg aacacatgga
     4321 aggtctgttt caacgttttt ctaaactgtt acctttcaga attgattttg cctacaggaa
     4381 agacacgccc agttttggtc attcctgtag gcacagtatg tgtattgaaa tgcaccgctt
     4441 acttagtgaa acccaaacga tgctggcggg atattactgg gtaatggaat atacgtcaaa
     4501 caagggactt catattcatt ttatcggcta cctggatggg caacgtcaca acaaatctta
     4561 tcggatatcc aggcaactgg gagatatctg gaggcggatc acggaagggg agggttattt
     4621 tcacctgtgc agggctaaag acaaataccc ggtgcgtatc gatcacgtaa ttcactatag
     4681 tgataaatct gcggtagacg acttacgtta cgcccttagc tatctggcca aacaggatca
     4741 gaaagaacac ggtattattc tgaggcgtag caggcttccc gaaaaaagta accggggccg
     4801 accacgacat aactgaatta cagctgtatt aggtattaga attaagtttc tgtaagttgt
     4861 tgggaggtgc ttgagttaat aggcagaaga gggcggggcg ctttgtcatc agtgtagggg
     4921 acccgaattt ctgccgtggt gcctcagtgg atcggataca ggtaacgaat atggttcata
     4981 aatctgacag tgatgaatta gctgcattga gggcagaaaa tgcccgtctg gtctcattac
     5041 ttgaagctca tgggattgaa tggcgacgta aaccgcagac tcctgtgcag cgcgtttctg
     5101 tattatccac cgacgagaag gttgcattat ttcgtcggtt gtttcgtggg cgtgatgatg
     5161 tatgggcact tagatgggaa agtaaaacca gcggcaaatc agggtactct cctgcctgcg
     5221 ctaacgaatg gcaggcgaga atatgcggta aaccccggat aaaatgtggg gactgcgctc
     5281 accgtcagtt gattcctgta tccgatctcg tcatctacca ccatctggcc ggtacccata
     5341 ctgtcgggat gtatccgttg ctggaagatg attcctgtta ttttctggca gttgattttg
     5401 atgaagctga atggcagaag gatgcatccg cattcatgcg atcctgtgat gagctgggtg
     5461 tacctgctgc gctggaaata tcccgttcac gtcagggggc gcatgtctgg atattttttg
     5521 cctcacgagt ttcggcccgc gaagctcgcc gtctggggac tgctattatt agctatacgt
     5581 gtagtcggac ccgacagctg cgattggggt cttatgaccg attatttcct aatcaggata
     5641 ctatgccaaa agggggattt ggtaatctca tagcgttacc tctgcaaaaa agaccgcgtg
     5701 aattgggggg aagcgttttt gttgatatga atctccagcc ttatcctgat cagtgggctt
     5761 ttcttgtatc ggtgaccccg atgaatgtgc aggatattga accgacgata ttacgggcta
     5821 cagggagtat ccatcctctg gatgtgaatt ttatcaacga agaagacctg ggtacgccgt
     5881 gggaagagaa aaaatcatca ggaaacagac tgaatatttc tattgcagaa ccgctgaaaa
     5941 tcacgctggc aaaccagatc tatttcgaaa aagcgcaatt acctcaggtg ctgattaacc
     6001 gacttattcg gctggcagca tttccgaacc ctgagtttta taaggctcag gcaatgcgta
     6061 tgtcagtctg gaataagccc cgtgttatag gttgtgcgga gaattacccg caacacattg
     6121 cgttgccacg gggatgtctg gacagcgtat tatctttcct tagggacaac aatattgctg
     6181 cagaattaat cgataaacga tttgccggga cggaatgtaa tgccgttttt atgggaaacc
     6241 tcagagcgga gcaggaagag gccgtttcgg cattactccg ttatgacact ggtgtgcttt
     6301 gtgcgccaac ggcttttggt aagacagtta ccgcagcggc agtgattgcc aagcggaaag
     6361 tgaatacact gatactggta caccggactg aattgctgaa gcagtggcag gagcgtctcg
     6421 cggtgtttct gcaggccggt gacagtattg gtattatcgg gggaggtaaa cataaaccct
     6481 gtggcaatat tgatattgcg gtggtgcagt ccatatccag acaaggagaa gttgaacctc
     6541 tggtcaggaa ttatgggcaa atcattgtgg atgagtgcca tcatattggc gcggtttcat
     6601 tttctgcgat tctgaaggaa acgaatgcca gatatctgct tggcctgacg gcaacaccaa
     6661 tccgacggga tggtctgcat cccattattt ttatgtactg tggtgccatt cgccatacag
     6721 cggtccgccc gaaggaaagc ccacataatc tggaggtact gatccgttcc cgttttacat
     6781 ctggtcattt accatcggat gcgagaatcc aggatatttt cagagaaatt gctctggatc
     6841 atgacagaac ggtggcgata gctgaagaag ccatgaaagc tttcgggcag gggcgaaaag
     6901 ttctggtact gactgaacgt acagatcatc tggatgagat agcatcagtg atgaattcac
     6961 tgaaattgtc tccctttatt ctccatggtc gactatcgaa aaaaaagcgt gcgatgctga
     7021 tatccgggct gaatgctctt cctcccgatt ctcctcgaat tttgttgtca acaggcagac
     7081 ttattggtga gggatttgac caccctccgc tggatacgct gattcttgcc atgcctgtgt
     7141 catggaaagg gacattacag cagtatgcag ggcgtcttca cagagagcat accggtaaaa
     7201 gcgatgtcag gatcattgat tttgtggata ccgcgtatcc tgtgctgctc agaatgtggg
     7261 ataaacgtca gcggggttat aaagcgatgg ggtacaggat tatagctgac ggcgatgaat
     7321 cagtgattta attttaagga gtactttacc ctttttaaat aatttgagct ccccgggttg
     7381 ttcgggcaac aaagagtccc agtatacagg cttcaggaat atgttgtagt atcaggcttt
     7441 tcatcgcttt aaaatgacta ccggcagtga gaacgtcatc aaaaataaca atctagggcc
     7501 tgcacccttc cagtaattgt tcatccagtt ggtagtttgc cataatcgct tctggtaagg
     7561 gccggtttgt tgcggtatga gaggctattg ttgatacgtt ttgcacaacc agttcccaga
     7621 aatccagcca tggttcgtag gtttgcgcaa ttcgcagaat ttctgtcata cggtcatcgt
     7681 aagcggcatc tgtacggcat ttggatggtg gtacgggaac aaatgtgtat tcctcaagat
     7741 taccgatatt tgatatgagc atctgagcaa catactcaat agcagccttt ttatagtggt
     7801 aatcaggcta cgaaagtaca gcgttttata aattgtctca ggtgatattc gcagcgtttt
     7861 ttgacgtggt tttgttcgcc ttaaccatcc tgatatttgc tctggagacc atttcatctc
     7921 cagcttttcc agaacaagct ttcgcaatgg taaattttga tccagtaagc acggttttgg
     7981 ccttttcgcc attctgttgg ctcggttatt agcatcaaca gctttgtaat agcgtctgcc
     8041 ccgattacgc gtaagcgtac agcgagggcc gtatt
//
LOCUS       DAZUFB010000160         8074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZUFB010000160 DAZUFB010000000
VERSION     DAZUFB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.160"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(419..1180)
                     /gene="envY"
                     /locus_tag="ACHY3M_004569"
     CDS             complement(419..1180)
                     /gene="envY"
                     /locus_tag="ACHY3M_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFS4319337.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKVKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1363..2253)
                     /locus_tag="ACHY3M_004570"
     CDS             complement(1363..2253)
                     /locus_tag="ACHY3M_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFS4319338.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2254..5226)
                     /gene="nfrA"
                     /locus_tag="ACHY3M_004571"
     CDS             complement(2254..5226)
                     /gene="nfrA"
                     /locus_tag="ACHY3M_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFS4319339.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSTEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5213..7450)
                     /gene="nrfB"
                     /locus_tag="ACHY3M_004572"
     CDS             complement(5213..7450)
                     /gene="nrfB"
                     /locus_tag="ACHY3M_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFS4319340.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRSLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7719..>8074)
                     /locus_tag="ACHY3M_004573"
     CDS             complement(7719..>8074)
                     /locus_tag="ACHY3M_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFS4319341.1"
                     /translation="VAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAM
                     DRNYLASVLAGSGLS"
BASE COUNT         1878 a         2054 c         2106 g         2036 t
ORIGIN      
        1 tgtttagtat tgaatgatta tcacaatgcg ctatctgttt ttggtttaat tatctgttat
       61 tgttttatgt tgcggtttta ctgtgtggtt ttttatgctt ttgtggtgtt tttatctatt
      121 taagcgccat gcctttaaag gcatataagc gaaaatagca tgaggtttat cctcaattgc
      181 tatgtttttt agtacaaaaa agagggacaa aactgagaca cataaggcct cacaatggct
      241 tgcaaggctt tacatgtttt gaggtagtgg gacgtgtgag cgcagagatg gcgcggtaat
      301 ttgttgactt aaaatgtcgt tctaggaact tctaagtcgt gggccgcagg ttcgaatcct
      361 gcagggcgcg ccatttcttc ttcatttatg cccgtcttat ccgtttccgc tttgcccttc
      421 accacatcac tttttgtcgc tgtttggcga gataattcaa cggtgtcagg ccgtaaaacg
      481 ccttaaaaac agagataaag tacgacgtgc tgctatagcc acataattgc gccacctgag
      541 tgatattttt gttatccatc aataacatct gtacggcgta acgcatacga cactctgtga
      601 caatctggct atagctggta ttttcgtttt ttactttctt tttgagtagg ctggggctta
      661 aacatagcga actggcgaca attcgcagat tccagtaatg ctgaatatcg ctttgaataa
      721 tgcggcagac gctgtcgcgg acgctgctgc gtaagatata catcagtagg gctataaacc
      781 gcgattgctc aagaaagtta gacaatacgg taaaaagtaa tgcgcgcgtc aactccgctt
      841 cgcagggttt gctgggttgc tgcttgctgt ggttggcggc tagccggaac acttccggtg
      901 tacggctatg acaagcgata accggggttg ccagccgcga ccagggcttt acacaggtta
      961 agttcttatt taaaaacaac agataatcgt tgatgatgtt acggttgatg tgggggatta
     1021 atacgtgatt taacgttgag agttcaatga cgttattgtt gcacgcgaag gcggccaggt
     1081 agtttttcgg aagggtaaac gtagcatggt tattgacgct tacctctacc tctttttcgg
     1141 tcaggatcac cacgcaaggt tcactgctgc tcaattgcat ttcgcactcc tcagatatca
     1201 gaaactccgc tcaaaggatc tatgcttcct gcatgagtga tcggcccgtt cgccgataac
     1261 gatcttcttt ctttagcacg ctttttagca attaatcttg atggaattct gacgagagcg
     1321 aaagaggtaa gccaggtcgt acccgactta cctggaggag atttaatact cgagaatgcc
     1381 gtgcgcgacg ggcaaatagc gcagagagaa atagagagtg tctttaccgc aagaggaacg
     1441 tttcgctaac agcgaggcga tctctgcatc cggtttcggt ttcgctgtaa aggttttgcc
     1501 tttgccggcg acaaacagtt cataaacaat gccgctggca ggggcgggac tttggcaatc
     1561 ggcgctggcc tgtagataac gttcacgctg ttcagcggta agtttatcca cgccgctgcc
     1621 atcctgtacc cagacattaa cgccggttgc tttaacttgt tccagcagtt ggcgatagcc
     1681 atcgggcgac atgtttccgg cgaaaaaact actgatataa accggttttg ctgaaacatc
     1741 gctaatcagc cgctgcgcgt tgtttaacca tgttagcaaa ggctgacggg cagcttcgct
     1801 gcgccagttc aggtcgtcaa tttccgcgct gatgtaccag ccatccggcg ttacgccagg
     1861 cgcggcgctc cataatctgg cttgctggag atcggcagcc agcaggcgat taagatagct
     1921 ttccagcgct gcggacgact gtttctggtg cataaaaaat tccggatcgg cgttcagccc
     1981 gacaatcagc ttcaggccag cctgttgcgc agctgcggca cgcttaaaca ataacgcgcg
     2041 ctgttctggc tgggtgaatg catcgccgta acgggtccat tgcaaaacca gggtatcgaa
     2101 gccttgcaaa cgtaactgac tcatcagccc ctgccactgg gtatcggtaa cctgactatc
     2161 tcggttttgt ggttgccaga taataccttt catcgcaaag gaaaaagggc tgaccaaaag
     2221 cagtgtcagc aatacgaaaa tgaacttacg catttaccag tgcactccaa tggtgagaaa
     2281 cgcgttgttg cgctctccgt tacgttgatt aatcgcctta aaggtatgtt gatactcgac
     2341 gccgagactg actttgtgcg gccaggcgtc gtagtgcgtc tcgccggtcc agatattcca
     2401 gcggaccccg actccgccaa gctgcgcgcc ctgagtgcct ttatcacgat agccgttgtc
     2461 ctgaacgtga gcgtaaggct caatagtctg tccgttagct accttctgat gccagctgac
     2521 gcgataatct gccgtccacg cctgaatatc ctggcggata tattgcgccg catcgaggta
     2581 caggttttgg gcaaaccagc ctgaaccgtt cgggtgccat tcgtcgctgt atttgccgcc
     2641 attaaagaat gaggcgctgg cgcgcagcat ggtatcggat gcgccatttt ggccgttcag
     2701 cggcaactgc tgttcgacgg cgaggaaaaa gatctgatcg cgcagcggct tccagcgcag
     2761 accggtgccg gacatcggat ttttcaccgg catcatcacc ccgttttctc cggtatcggc
     2821 aaagacgcgg ctataaactg agagcaggtc gccttccagc agcatattgc gtccgatgcg
     2881 gtactcggct tccagttgcc cgtagctacg atagcttttc cctggcgctg cgccgccgac
     2941 attattgtta gcggtgctca ttgccccgga acgcaagccg atagaagaat cgaaactgaa
     3001 cgtccagcgg cgaccgacct cctcatgcaa acggcggaaa ttgaagcgtt gctgattttg
     3061 ctctggggtc agtggggtta tcagcgcctg attatcaatg tcatcaatca ccagccgggc
     3121 gtagtgctgc gtcgcaggca tgtcatccag acgctggttc acgtaggcca gttgtcggat
     3181 cagtgccgga tcgtccggaa gccctttatg cgcctgttcg agcatttccc gcgactgtgc
     3241 gatatcaccg ctatcccaca aggcgtaacc aagcgctgcc tggatgttgc tattattcgg
     3301 ttccagttcc agcgcggcgc gcaaatcact caccgccgcc gggacattat gacgttggcg
     3361 ataaattgtc gcccgcgcaa cgtaagcgtt ggcagaaggc gcaatattga ttgagcgcgt
     3421 gagatcgctc agtgcgagtt ccggctgacc aggaatgtaa cgttgcgcat gcagccacca
     3481 gtagagggca ttgtttccca gtccacgttg ttctgcctgt tgtagccagc gatcgcgagc
     3541 tgcaccattt cctgccgcct gggcggtatt ggcagcagca agcagatcct cattgctcat
     3601 gtcgtgaaga ctgattttct gccaggccgc cagtgcggtg gcgtagtcct caacctgata
     3661 cgcctgatag gctaccgcac gatgttgcca ggcgttcggt tgtcgttgtt cggcctgaag
     3721 ccatgcatac aaggccacac cgggtagcgt gtcccgataa cactttgcca gacggttcca
     3781 ggcggcggca tcgtaggaag gcgacatatc gcccagcaag cgaactattg ccgggcaatt
     3841 atctgcaata cccggcaact ggctttgcca ctgacgttgc gccgccagcg gcaagggttt
     3901 tgataaaatc gccaccttcg ctggagttgc caggtaagga tgactttcca gcagagacgc
     3961 cagtcgcgcc attaaagtct ggctgacacg cgcatcgccc tggaaaggat agcgttgcag
     4021 caataaatcg gcagcttcgc gtgactgctc gttctgcatc agttgccagg tcagttgatc
     4081 caggcgggta agatttgccg gttcttgctg atacagcaat cgtgccagac gcagagattc
     4141 agccttgtta cgggtcgcca cgctgacagc ataacgctcc tcaagcattt cattggcggg
     4201 gagggtggcg agcagttttt gcgctgcgtc gtactgacct tcttttaaca gcaccggtag
     4261 cgtcgcgcca acaacatact ggcggttatc ggcaaactgc accgtataat tcgccaatgc
     4321 ctgaacgggg ttggcgctgt atttagataa cagatagagc caacttttct cttgtgcgtc
     4381 cgtggtaaac agcggcttat tttcaatgag ataatgctgg aggcgtgctt tttcgccacg
     4441 ataagccagc gcggtcgcgt aagtaatata tgactgagga tcggtgaaga tcccctgtga
     4501 ttgcagtgcc aggatccgat cgtccagctg cccggcaaga agcacgtcaa accactgacg
     4561 gcgttctgcc gcgcttaatg tgttctgctg gcgtgcttca ttgtatagcg tatctgcctg
     4621 ggaccattgt ttcaggtaga ttgcccgttg cagcagatcg gttcgcagcg tttttccttc
     4681 cggcgatgca gcaaacgtcg catcgttcag ttgcgctctg gcgacaggta actgtgccag
     4741 ccgcagggca ttctgcccga cttcactgcg acaacgcagg gtcggcgcag catcgcacgc
     4801 tttttgctgg gcaagcagtt cttcaacagt cgtaacgctt ttcacttcaa ctggaatagc
     4861 cgccagactg cgctcaagtc gggcatctcc tgggtgacgt ttcagttgat cctccagcaa
     4921 cagccgcgcc cggtcatcat gaccaaaatg gcgataggct tccgcaaggt ataaagtcag
     4981 cggaatatta tccggcacct gctggtgtat atattcaaat tcgcggatgg cggttgcttc
     5041 gtcgttattt ttctgtgcct tcagcgcctt atcgagacgg ggataaataa caaaatggcg
     5101 ataatcgctc agccccagct cttctgtgct ggtgccgata ttgtctgcga gtgcgctggt
     5161 actcaataaa gacgtcagca gtaaaccaga ccatccgatg acgcgattaa ggttattctc
     5221 cttcattttc ggactccagt tgcgcaacct gttctgtgtt taaacctgct ttgagtaata
     5281 gtgattgcat cgaaacttgt aattcgcgtt gaattgtcag gacgcgatcc aacgtttcct
     5341 ggctgataac gccttcggtg accaaaaact tgccgagcgg cagagaactg cgttcatggc
     5401 gcaataacaa cacgttaatt gctgaacgat taatatgacc gagcgtggtc agtatttcgg
     5461 cgaacaggaa ctgatgcggc acatattgcc gccagatttc accggtctgc tgttccgtga
     5521 gccactgatg ctgaaccgca ttgtacaaca ttgcccgcgg atcgtgaccg cgtcggcgtg
     5581 cataccagtg acgtaacccc gtgacaattt gtccccgcag aacaatgacg taacgcactt
     5641 tgcgtccgac tttacgcgtc agggccgcca gcgaaacagg gtcaatacca tcttcactgc
     5701 cgacaattaa ctcatcattt tccagacgca gcggcagtac tgcataatgc agcgctacgg
     5761 aggctggcat ttcggcaatc agcgaggacg ggatctgcca ggcatcgatg gattcccacg
     5821 ccacgccgtt ttgctctgcc agcgcctgtg ccagctgctc ggcgctaatc agcccctgca
     5881 tcagcattga accgcccagg cgtagacctt cgacgcgatt acgcagtgct gtatcgagtt
     5941 gttcttcagt gatgacctga ttttccagca gaatttggcc taacgagcgc aacgagcggg
     6001 tatcgccagt cacgctgggg aagtcatgcg ttgttttatc ccacgcgacg cgacgtggat
     6061 cgccgtgttg aagtacctgt tttagcgcgc gccagttggc catgaagtta atcaggttgc
     6121 cccagaaaag acgcaggaca gatagcagcc cctgcgtcag accgtagtag ccagtgacga
     6181 aaatcacccg ctgcacgatg cggttaaaca tcaagccaaa gtttagccac agcagggtca
     6241 ttaaccatgc gctgccgcta aaaatagaaa ggaaatgcca ggcatcgggc cacaaacttt
     6301 catacgccag caacagcaaa agctggagca tcaccagcat cgcgaggaag ctgacaaagt
     6361 tactgattgc ccctttgcgg tcgcgccaga gaaagtagtt cagcgtcagg ctagaggtcc
     6421 atttgtgggt tttaaagccc tggaaaacaa tgccgatgat ccagcgagat ttttgtcgaa
     6481 ctgcagtcga aaaggtatcg gggaaatatt cgcgcacgca gatcatgttt gacgtccgtg
     6541 cgtgctgtaa aaatttacgc tgctcgcgtt ctttggcttc gtccaccacc ggaaaacgga
     6601 caaaaatttc cgtcatacct ttctctttca ggcggaaacc aatgtcgtaa tcttcagtca
     6661 gactctgcac gtcgaaagca ataccgtcac cgtcagctaa cagagcggtc acggcgcggc
     6721 ggctgaaaca ggtgccgacg cctgcgctgg gcacttgtcc ggcgagggct tcacgcaccg
     6781 gaacatcttt gccatgcagc tctgaaaact catcaatgta agtcatgctg gtgaagtgcg
     6841 tccattcgcg ttcgaacgga tacaccggga tctgaatcag atctttacgc tcgaccagat
     6901 agttgaacag acgcaattcc atcggtgaaa tcacatcttc ggcgtcatgc agaataaaac
     6961 cagcaaaagc gaaattggcg ctacgttcaa actgggtgat ggcgtccagc acgttgttca
     7021 gacagtcggc tttgctggtg ggaccaggac gcgcgcagac taccttatgc acgttcggaa
     7081 agcgagcgca cacttcgtca acatcacgtt gagtatcggg gtcgttgggg taggtgccaa
     7141 caaagatatg atagttttcg tagtcgagcg tggtcgccgc cagctcggcc atattgccga
     7201 tgacgcccgt ttcattccac gccggaacca taatcgctaa cggtttttca tctggtttat
     7261 acagttcgcg gtaactcatt cgcgggtagc ggcgataaac actcaacttg cgtttaatgc
     7321 ggcgtaccca gtagacgaca tcaataaaaa aatcgtccag cccgctgatg aacatgatga
     7381 ccgctaacgt tatcgcgatt acttttaagc cgtagagcca ggttgcaaaa acatcaagaa
     7441 gccagtccac acaaaaacct tacattaacg ctggttatgt ttagggtggc gtatattaag
     7501 gttttttatg aattgtgaca gctttttacc attaataggt atgactattg cggcacgtta
     7561 tttttacact ggttataaaa gttgccgttt gcgtagaatg tagacaaaag catggtgtca
     7621 aggaggggag gttaacgtca tagtgaaaag gagcggtggc accgagcagg tcataatgta
     7681 gtggaagtgc ctggaggagg tgagcaggca gggcaagatt acgaaagccc gctccccgca
     7741 aggactgacg cgagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct
     7801 gccttgaata ccttatcatt cgttaaaata ttaatagcga tgtgccgtat ccctgaaaat
     7861 aattctgcgg cgtttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga
     7921 cgccagtgca gcttattctc cacgtgccag tggtttcgga ttgctgtggc gaacttctct
     7981 gcggttaaat cagcagaact gatataatat ctgaccgtca tttcgggctc tttcttttgt
     8041 tctgctatta ttgaccgaaa ggagactgcc acgc
//
LOCUS       DAZUFB010000161         7951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZUFB010000161 DAZUFB010000000
VERSION     DAZUFB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.161"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..384
                     /gene="dinJ"
                     /locus_tag="ACHY3M_004574"
     CDS             124..384
                     /gene="dinJ"
                     /locus_tag="ACHY3M_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFS4319342.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            387..665
                     /gene="yafQ"
                     /locus_tag="ACHY3M_004575"
     CDS             387..665
                     /gene="yafQ"
                     /locus_tag="ACHY3M_004575"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFS4319343.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            821..1561
                     /gene="dpaA"
                     /locus_tag="ACHY3M_004576"
     CDS             821..1561
                     /gene="dpaA"
                     /locus_tag="ACHY3M_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFS4319344.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1532..2299)
                     /locus_tag="ACHY3M_004577"
     CDS             complement(1532..2299)
                     /locus_tag="ACHY3M_004577"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFS4319345.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2505..3083)
                     /gene="lpcA"
                     /locus_tag="ACHY3M_004578"
     CDS             complement(2505..3083)
                     /gene="lpcA"
                     /locus_tag="ACHY3M_004578"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFS4319346.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3323..5767
                     /gene="fadE"
                     /locus_tag="ACHY3M_004579"
     CDS             3323..5767
                     /gene="fadE"
                     /locus_tag="ACHY3M_004579"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFS4319347.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5810..6256)
                     /gene="ivy"
                     /locus_tag="ACHY3M_004580"
     CDS             complement(5810..6256)
                     /gene="ivy"
                     /locus_tag="ACHY3M_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFS4319348.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6437..7204
                     /gene="yafV"
                     /locus_tag="ACHY3M_004581"
     CDS             6437..7204
                     /gene="yafV"
                     /locus_tag="ACHY3M_004581"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFS4319349.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7428..>7951)
                     /locus_tag="ACHY3M_004582"
     CDS             complement(7428..>7951)
                     /locus_tag="ACHY3M_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFS4319350.1"
                     /translation="FEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELID
                     FTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVEN
                     KLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDR
                     NYLASVLAGSGLS"
BASE COUNT         1910 a         1979 c         2069 g         1993 t
ORIGIN      
        1 aataaatata tttcagttaa ttatgtaaaa acatccgttt attaaattac tcatggattg
       61 tcgctgttgc tcatttgagc tacaattcaa gctgaataaa tatacagcac aggagatacc
      121 ccaatggctg ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa gaatcaggcg
      181 gcggacgtac tggccgggat ggggctgacc atctctgacc tggttcgcat aaccctcaca
      241 aaggtcgcgc gtgaaaaggc attgccgttt gatttacgcg agcctaatca attaaccatt
      301 caatcaatca aaaacagcga agctggcgtt gatgttcata aggccaaaga cgccgatgat
      361 ttatttgata aattaggaat ttaaatatga ttcaaaggga tattgaatac tcgggacaat
      421 tttcaaagga tgtaaaactt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc
      481 ttatgacgct tcttatcaat aatgctttac cgcttccagc tgtttataaa gaccacccgc
      541 tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt
      601 acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg
      661 ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc
      721 ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat ctattttgcc
      781 gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat
      841 tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc
      901 gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca
      961 gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcagct
     1021 gctcgacagt tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg
     1081 cgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag
     1141 ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta
     1201 tgaagggaaa tacctgatga tccacggcga ttgtgtttcc atcggctgct acgcaatgac
     1261 caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc
     1321 gagcgtgcaa gtgagtattt acccgttccg catgaccgac gccaatatga agcgccataa
     1381 atattccaac tttaaggact tctgggagca gctgaagccg ggctacgact actttgagca
     1441 aacccgtaag ccaccgaccg tttctgtggt caatggccgt tacgtggtca gcaagccgtt
     1501 aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata
     1561 agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg
     1621 caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct
     1681 gatccagcag cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg
     1741 agcaatacgc catcacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc
     1801 gcagttcatc cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc
     1861 gcgggtaacg ctgcgttaat ttatgcagga gccagcagaa ggctttttcg ctatcggttt
     1921 caccgaccgg gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccgttat
     1981 gggcataagt ccagttacgc ccccataact cgcgggtaaa tgggtgagta ttttccagcg
     2041 ccacctcgcc ccgattagcc tggcgaatat gagccaccac cgaacaggat tttatcgggt
     2101 agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct ttaaatgtgc
     2161 gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac
     2221 caccacgctg tacaagtccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca
     2281 tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgaatgg cccccggcga
     2341 agattgattt aagaattttc aggtcggatg cggcgtaaac gaattattcg gcctacggtt
     2401 cgggtacaga tttgtaggca tgataagacg cgtcagcatc gcatcaggca tcagcgcaca
     2461 agtgccggat gcggcgtaaa cgccttatcc agcctacgcc agacttactt aaccatctct
     2521 ttttcaatta actggatcag gatatggatc actttaatgt gaatctcctg aatgcggtcg
     2581 gcataaccaa agtgcggtac gcgaatttcg atatccgccg tgccagccat tttgccgccg
     2641 tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgctgc gatcgctttg
     2701 atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc ttcgcgacct
     2761 accgcttcaa cgtagcggga gaaaatatca ttgaaaccga aatcattacc gacgcaggaa
     2821 atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc acggtagcga
     2881 ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa
     2941 agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg ctgaatggcg
     3001 tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc gttcagttcg
     3061 ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat agacaaaatg
     3121 cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac caggactttt
     3181 tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt atattgtaaa
     3241 tacattgcta aatgttttta catccactac aaccatatca tcacaagtgg tcagacctcc
     3301 tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct
     3361 cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt tgctcgcctg
     3421 gacagcctcc ctcggcgttg ctggtctgtg gtcggcgtgg gtactggtgc ctctggccat
     3481 tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt
     3541 tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag cgattgatgc
     3601 gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga aaaagctgca
     3661 taactatccg cagccgcgcc tgaccgccga agagcaagcg tttctcgacg gcccggtaga
     3721 agaagcctgc cggatggcga atgatttcca gatcacccat gagctggcgg atctgccgcc
     3781 ggagttgtgg gcgtacctta aagagcatcg tttcttcgcg atgatcatca aaaaagagta
     3841 cggcgggctg gagttctcgg cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt
     3901 gagcgggatc ctggcgatta ccgtcggcgt gccaaactca ttaggcccgg gcgaactgtt
     3961 gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca
     4021 ggagatcccc tgctttgcac tgaccagccc ggaagcgggt tccgatgcgg gcgcgattcc
     4081 ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg ggatgcgtct
     4141 gacctggaac aaacgctaca ttacgctggc accgattgcg actgtgcttg ggctggcgtt
     4201 taaactctcc gacccggaaa agctgctggg tggcgcagaa gatttaggta ttacctgtgc
     4261 gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt
     4321 accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg attacatcat
     4381 cggtgggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg
     4441 ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag cgctggcaac
     4501 gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga tggaagggat
     4561 tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg cggcatcgct
     4621 gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta
     4681 tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca ctggtggcaa
     4741 aggcattatg ctcgggcaga gcaacttcct ggcgcgtgct taccagggcg caccgattgc
     4801 catcaccgtt gaaggggcca acattctgac ccgcagcatg atgatcttcg gacaaggagc
     4861 gattcgttgc catccgtacg tgctggaaga gatggaagca gcgaagaaca atgacgtcaa
     4921 cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag
     4981 cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg atgccactaa
     5041 acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt ctgatgtctc
     5101 gatggctgtg ctgggcggca gcctgaaacg tcgtgagcgc atctcggcac gtctggggga
     5161 tattttaagc cagctttacc tcgcctctgc cgttctgaag cgttatgacg acgaaggccg
     5221 taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt atcaggctga
     5281 acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa
     5341 tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc tggatcataa
     5401 agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc gcggtcagta
     5461 cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg tggatgtgat
     5521 tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc tgccgtttac
     5581 ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata aagatgaagc
     5641 cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg atgactttga
     5701 tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga aagttgaagc
     5761 cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat tatttaaaat
     5821 taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc accgttttac
     5881 catcaatcga aagcgcatcg ttcacattca gccaggtgag tttctcttgc gacgttttct
     5941 catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac atcacagcga
     6001 tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac gtctcatctc
     6061 ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag gcaggcagct
     6121 tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt gcaaggctgc
     6181 taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg gcgactgtcg
     6241 ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta accttctaaa
     6301 gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca tttgttatca
     6361 atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa cccttcgcat
     6421 aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac cactggtgtg
     6481 gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg
     6541 cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag cggcagcttc
     6601 gtcgctagca caagatgacg tagtgaactg gatgacagct aaggcgcagc agtgcaatgc
     6661 gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc gctttttgct
     6721 ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc gcatggcaga
     6781 tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc gcggctggcg
     6841 tattttgccg ctggtgtgct acgacttacg ttttcctgtg tggtcgcgca atctcaacga
     6901 ttatgacctc gccctgtacg tcgccaactg gcctgctccg cgctctctgc actggcaggc
     6961 attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg
     7021 cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc cacaaggcga
     7081 gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgccgagc tgtcgatggc
     7141 ggtgttaagg gaatatcgcg ataagtttcc ggcatggcgg gatgcggatg agtttaggtt
     7201 gtagtgagtg ggggctgtat ttagggcggg gaattcagaa agggatgttt agtgtgctga
     7261 gcgagagtta aataataaaa aactgggaag atttaataat ccccccgcag ggcaagagca
     7321 tgaattaaat ttgtgttaaa tttttcattt tatcagttcg aaacccaagc tctgagcagg
     7381 atgcaagcac ttcttctata taacatgtgt ttaacgccag ggcaagatta cgaaagcccg
     7441 ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg catcttacgt
     7501 cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat gtgccgtatc
     7561 cctgaaaata attctgctgc atttcctctt cttattttgc agtcgtcttc attcattacc
     7621 acgtccagac gccagtgcag cttattctcc acgtgccagt gatttcggat cgctgtggcg
     7681 aatttctcag cggttaaatc agcagaactg atataatatc tgaccgtcat ttctggctct
     7741 ttcttttgtt ctgctattat ggaccgaaag gagactgcca cgcataattt cttcagccct
     7801 ttccattcaa acgtgaaatc aataagttca tcagggacat cgcaaacaat atgaagacgg
     7861 atttcttctc tgccgtgact cttttcacta attgcgtaac tgtcatgctc tggattattt
     7921 aattctttca gcggaaattt ttcctcaaag g
//
LOCUS       DAZUFB010000162         7932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZUFB010000162 DAZUFB010000000
VERSION     DAZUFB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.162"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..918
                     /gene="tssI"
                     /locus_tag="ACHY3M_004583"
     CDS             <1..918
                     /gene="tssI"
                     /locus_tag="ACHY3M_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFS4319351.1"
                     /translation="QAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVV
                     TGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQE
                     VIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDAT
                     GREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITG
                     VLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPD
                     GIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            946..1407
                     /locus_tag="ACHY3M_004584"
     CDS             946..1407
                     /locus_tag="ACHY3M_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFS4319352.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITQAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1427..6226
                     /locus_tag="ACHY3M_004585"
     CDS             1427..6226
                     /locus_tag="ACHY3M_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFS4319353.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKQLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVVPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            6228..6593
                     /locus_tag="ACHY3M_004586"
     CDS             6228..6593
                     /locus_tag="ACHY3M_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319354.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIAFDYIKDMIVNVSKTERLDGYFRVNDDEDNL
                     FLEWIVKDGYLEEIILSGS"
     gene            6898..7335
                     /locus_tag="ACHY3M_004587"
     CDS             6898..7335
                     /locus_tag="ACHY3M_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFS4319355.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            7355..7678
                     /locus_tag="ACHY3M_004588"
                     /pseudo
     CDS             7355..7678
                     /locus_tag="ACHY3M_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            7829..>7932
                     /locus_tag="ACHY3M_004589"
     CDS             7829..>7932
                     /locus_tag="ACHY3M_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319356.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTI"
BASE COUNT         2067 a         1959 c         2310 g         1596 t
ORIGIN      
        1 caggcggtgc caggacggca gggagcgggg acggcgctgg agaaccattt tgcggtgatc
       61 ccggcagaca gaacatggcg accacagccg ttgctgaaac cgctggtcga cggcccgcag
      121 agcgctgtcg tgacaggacc ggcaggcgag gaaatcttct gcgacgaaca tggtcgcgtg
      181 cgggtgaagt tcaactggga ccgttataac ccggcagacc aagacagttc gtgctggatc
      241 cgtgtggcac aggcgtgggc aggcaccggt tttggccacc tagcgatacc gcgtgtgggt
      301 caggaggtga ttgtagactt cctcaacggc gatccggacc agccgatcat tatggggcgc
      361 acctaccacc aggaaaaccg cacccccggc agcctgccgg gaacaaagac gcagatgacc
      421 atccgctcca aaacgtacat gggcagcgga tttaatgagc tgaagtttga tgatgcgaca
      481 gggagagaac aggtctacat ccacgcgcag aagaacatgg ataccgaagt gctcaacgac
      541 cgtaccacca ccgtaaaaca cgatcaccgc gaaaccgtaa aaaatgacca gacggtcacg
      601 atccaggaag gtaaccgcct tcttacggtg gaaaaaggcc acaagatcac cggagtactg
      661 aaagggtctt tatctgagga tgtctttcag gacagaggca cgattgccgg ttcggtgcat
      721 gttgacgctg taaacaatgg tggcgaaggc gacggtatac aggcttatac ggcgattaag
      781 gaaattttgc tggctgtgga ggaaagcaaa attgcgctga cgccggatgg cattcagcta
      841 caggtcgggg aatcgacggt aatcaggctg tcgaaggatg gcatcaccat cgtgggcggt
      901 tctgttttca tcaactgatt cactcagatt atcagggagt attttatgca gttcactttt
      961 aatgaaggcc acattcaact gccgtctcaa tggcaggatc agtcaatgca ggtcctggtg
     1021 tccacagata acagtggcat caacctggtc attacccgcg aagccgttcc acagggaacc
     1081 ctgacaccag agctgtacca ggaaacgctg gcgctgtacc agggcaaact tgatgggtac
     1141 accgaacatg cctgccggga aatcactcag gcagaggccc cggcctggct gctggattat
     1201 tcctggaacg gtccggaaga tgaggggaat caggggcgga ttagccagat agccgtattc
     1261 cagcggcgtg gagatacgct gctgaccttc actttcagca cctccttatc cctgaagaac
     1321 agtcaaaaga cgatgctgct ggaggtgata aagagcttca ccccgttgcc accggagaat
     1381 gacatccaaa aggaccagcc gcgctgacat aaacgaggga taacacatga gtgaaggacc
     1441 tggcgggcca cagggagcga ccgcaggcgg tacgctggca atgcgaatgc tgtcacagca
     1501 ggcgatggtc gccagccaga tgaaacgggc agccaacgac aaagccattg cacagatgct
     1561 ggcagcaaag aagtccggcc cacctgccgc caggctgggc gatgaaattc agcataaaag
     1621 ttttctgggg gcactggcag gggccgtgct gggggcgata gtgaccatcg cagaaggttg
     1681 cctgattatg gccgcctgcg ccaccggccc ttatgcgctg gttctggtgc ctgcgctgat
     1741 gtatgccagc tataaggcga gtgattatgt ggaggagaaa cagaaccagc ttgaatcatg
     1801 gataaacagc ttttgtgaca cggacggcgc catcaatacc ggttctgaaa atgtaaacat
     1861 taacggaaag cccgctgcaa gggccgccgt cacccttccc cctcctcccc cacctggagc
     1921 aatacctgaa atcccacagg gggaaccctc atggggtgat attgccactg acctgcttga
     1981 atcggcagcg gaaaaagcag taccactggc gaaggcctgg gggaacgctg ttatcaccct
     2041 gacggaaagc aatgccggtt ttatggatcg cgtcagcgcc ggcgcatcgc ttctgtttcc
     2101 cgccggtccg gtattaatgg agtttgccac catggtgggc gggcgtagcg aaatcaaaaa
     2161 agatgtggat ttcccggaag ccggtgagga cacggcgctc tgcgacaagg agaacaaacc
     2221 accgaggata gcccagggca gtagcaacgt ctttatcaac aatcagcctg ccgcgcgcaa
     2281 gggcgacaaa ctggagtgca gtgcggcgat cgtgggaggt tcgccggacg tctttattgg
     2341 cggtgaacag gtgacctatc tggatatcca gccggagttc ccgccgtggc agagaatgat
     2401 cctgggggga ataacgatag ccagctatct tctgccgcct gcgggactgc tgggaaaact
     2461 gaagaatctg gcgagactcg gcaaactggg aaacctgctg gggaaaagcg ggaagcagct
     2521 gggcgcaaag ctcggcgcgt tgctggggaa aacaggtaag tcgttaaaaa gtattgccaa
     2581 taaagtcatc agatgggtaa cagatcctgt cgttccggta accggcgcat actgcgacga
     2641 acgtaccgac ttcaccctgg gccagaccct ccccctctcc ttcacccgtt tccacagttc
     2701 tgtactgccg ctgcatggcc tgaccggcgt gggctggagc gactcctgga gcgaatacgc
     2761 ctgggtgcgt gaacagggaa atcgggtaga tattatcagc cagggagcca cgctgaactt
     2821 cgccttcgac ggtgacagtg atacggcggt taacccgtat cacgcccagt acattctgcg
     2881 ccgccgcgac gattacctgg agctgttcga cagggatgca ctgagcagcc gcttctttta
     2941 tgacgccttt ccgggaatgc gtctgcgcca cccggtgact gacgatacca gcgatgaccg
     3001 cctggcacac agccccgacg accggatgta tatgctgggc gggatgagcg acaccgccag
     3061 caaccgcatc acgtttgagc gcgacagcca gtaccggatc acgggtgtca gtcacaccga
     3121 cgggatccgg cttaaactga cgtaccacgc cagcggctac ctgaaagcca ttcaccgcac
     3181 ggataacggc atacagacgc tggcgaccta cgaacaggat gcgcgggggc ggctgacaga
     3241 agcggatgcg cggctggact accacctgtt ttatgagtac gacgctgcgg accggatcat
     3301 ccgctggtcc gataacgacc agacgtggag ccgtttcacc tacgatgcac agggccggtg
     3361 cgtgaatgtc accggggcgg agggctatta caacgccacg ctggactatg gtgacggctg
     3421 caccaccgtg acggacggca agggcactca ccgttattac tatgatcctg acggcaatat
     3481 tctgcgggaa gaagcgccgg acggcagtac caccacgtat gaatgggatg aattccatca
     3541 cctgctggcc cgccactccc ctgccgggcg ggtggagaag tttgaataca acgccgcact
     3601 cggtcagtta agccgttaca cggcggcaga cggcgcggag tggctgtacc gctatgatga
     3661 gcgcggcctg ctcagcaaca tcaccgaccc tgccgggcag acatggacgc agcagtgtga
     3721 tgagcgcggc ctgccggtaa gcctggtgtc gccacagggc gaagagaccc ggctggcata
     3781 caccgctcag gggctgctgt cggggatatt ccgccaggat gaacggcgtc tgggcataga
     3841 gtacgaccac cacaaccggc cggaaacact caccgacgtg atgggccgtg aacaccacac
     3901 cgaatacagc ggtcacgacc tgccggtgaa gatgcgcggc cccggcggtc agtcagtgcg
     3961 gttacagtgg cagcagcacc ataaactgag cggccttgag cgggcagaaa ccggcgcaga
     4021 aggattccgc tatgaccgcc acggcaacct gctggcgtac acggacggta acggcgttgt
     4081 ctggacaatg gaatacggcc cgttcgattt gccggtggcg cgaacggacg gtgaaggcca
     4141 ccgctggcag taccgctacg ataaagacac gctgcagctc acagaagtca ttaacccgca
     4201 gggcgagtca taccgttata ttctggacaa ctgtggccgg gtgacggaag agcgtgactg
     4261 gggcggcgtg gtctggcgtt accgctatga cgctgatggc ctgtgtaccg ccagggtcaa
     4321 cggcctggag gaaaccatcc tctacagccg ggacgccgca ggccgcctgg cagaagtcat
     4381 cacgccggaa ggcaaaacgc agtatgcgta tgacaaatcc ggcaggctga cgggtatctt
     4441 cagcccggac ggcacatcac agcgcaccgg ctatgacgaa cgcgggcggg tgaatgtcac
     4501 cactcagggc cgacgggcca ttgaatacca ctaccccgat gaacacaccg ttatccgctg
     4561 tatcctgcca ccggaagatg aacgcgacag acaccccgat gaatccctgc tgaaaaccac
     4621 gtaccgctac aacgccgccg gagaactgac agaggtcatt ctgccggggg atgagacgct
     4681 gacgttcagc cgtgatgagg cgggacgtga agtgctccgg cacagtaacc ggggttttgc
     4741 ctgtgaacag ggctggaatg cagccggcca gcctgtcagt cagcgcgccg gattttttcc
     4801 ggaggaaacc acatggggcg ggctgctccc ctcactggta cgggagtacc gttacgacag
     4861 cgcgggcaat gtgtcggctg tcaccagccg ggaagattac ggacgggaaa cccggcggga
     4921 gtaccggctg gaccggaacg gccaggtcac ggcggtgaca gcctcaggca ccgggctggg
     4981 ctatggcgaa ggcgatgagt cctatggcta tgacagttgt ggctacctga aggcgcagtc
     5041 tgcgggcagg caccggataa gtgaagagac tgaccagtat gccggaggcc accggctgaa
     5101 acaggccgga aacacgcagt atgactatga cgccgcaggc cggatggtca gccgcaccag
     5161 acaccgtgac ggctaccgcc cggaaacaga gcggttccgg tgggacagcc gggaccagct
     5221 gaccgggtat tgcagcgcac agggtgagca gtgggaatac cgccacgacg ccagcggcag
     5281 acgaacagag aaacgctgcg accggaagaa aatccgcttc acgtacctgt gggacggcga
     5341 cagtattgcg gaaatccggg aataccgcga tgataaactg tacagcgtac ggcacctggt
     5401 gtttaacggc tttgagctga taagccagca gttcagccgg gtacggcagg cgcatccgtc
     5461 ggtggccccg cagtgggtga cgcggacgaa tcatgcggtg agcgacctga cgggccgccc
     5521 gctgatgctc tttaacagtg aaggtaaaac cgtctggcgg ccgggacaga ccagcctgtg
     5581 ggggctggca ctcagcctgc ccgcagacac cggctacccg gacccgcgcg gggagtggga
     5641 cccggaagcc gaccccggcc tgctgtatgc gggacagtgg caggatgcgg aatcggggct
     5701 gtgctataac cggttccggt actacgagcc ggaaaccgga atgtacctgg tgagtgatcc
     5761 gctggggttg caggggggtg agcagactta ccggtatgtg cctaatccac tggggtatat
     5821 tgatccgctg gggctggcaa aaacttctgt tcccgcagaa aaaataagcc tttcggataa
     5881 agcaagagat ctttttagac aaggaaaagt aagagaagca ttagatgttc attatgagga
     5941 tcttgtacga agaaagcttg gtggtatatc tcaagaaatt gccgggcgtg aatatgatgt
     6001 tgttactgac aagatcatag cgcaggtcaa gaggacttat agctctatag ataatccaaa
     6061 aaacttctta agtaagtcta ctcggacaca aattaagaag acaattgagc tggctgaaga
     6121 acaggggaaa gaggctcagt tttggtttaa atatggagta agccctaaag taagagaata
     6181 tatagaaagc aaaggtggga aagttatatt gggtatgggg aattaacatg tcaagatata
     6241 tacttattag aggttggttt gagtgcgact ttgaacaagt aaaaaaaata aaagaagcta
     6301 atgaagaatt tttcaaacat tctgaaaaat atttattacc agaaaacacg aaacgacttt
     6361 atttaaatgg gtggtgttac ccagaatatc ctatcaactg ggttagcgtt atttccttag
     6421 gtgttaatat aaatcaaatt gcatttgatt atataaaaga catgattgta aatgtttcaa
     6481 aaactgaaag attggatggt tattttcgtg tgaatgatga tgaagataat ttattcttag
     6541 aatggattgt aaaagatggt tatttagagg aaataatttt gtctggttca taaattcatc
     6601 ttctttgttg agaagattaa ctgcctaggt gaaattatcc tctatgaccg ggacgctgca
     6661 ggccacctgg cagaagtcat cacgcaggaa gacaagtcgc tgtatccctt tgaaaaatcc
     6721 ggcaggctaa tgggcatctt cagcccaggc ggcacatcac agcgaatttg aaggcaaatg
     6781 cgaaaattag tgatcttgaa gttcatacag tattggtaaa aacaggcgaa gcttttccaa
     6841 tgtgtaagaa ttttcaaatt tcaatatttg gcgctgttgt tacaagcggg tgattatatg
     6901 tgtgaattaa ttaatgaagc atcaaaatat gggaaagtca atgaaagaaa gatggacatt
     6961 gatttgtgct gtaaaaaaat agaggaagaa ggatattctg ttttcgttgc gttctatgat
     7021 tttataaaaa aatatggcgg tttatatggc tttcattcag cttacaaaga caccactata
     7081 aatgaggctt tttttataga tccagaaagg gcctctaatc gcatttttat agaaagagtc
     7141 aatgattata ttgagagagc tggttacaat atgattcctg ttggcgagtg cagtaatggt
     7201 catatcacat tattatatgg ggaaaataat gtgatatatg gcggcttgga tgattatctt
     7261 tggaagtatg gggataattt atcttcatca attgatatat tgattaatgg caaggaggca
     7321 gttgaaattc cgtaaggcca acttgatatg acacctggca cacaaccccg acgaccggat
     7381 gtatatgctg ggcgggatga gtgacaccgc cagcaaccgc atcacgtttg agcgcgacag
     7441 ccagtaccgg atcacgtgtg tcagtcacac cgacgggatc cggcttaaac tgacgtacca
     7501 tgccagcggc tacctgaaag ccattcaccg cacggataac ggcatacaga cgctggcgac
     7561 ctacgaacag gatgcgcggg ggcggctgac cgaagcggat gcgcggctgg actaccacct
     7621 gttttatgag tacgacgctg cggaccggat catccgctgg tccgataacg accagacgtt
     7681 gagccaggga aagatcatgt aacgccggtt ttttgtacta aataattcgc actaaatgtt
     7741 aaataattga gatattcctt attatctaaa actgtttttc tcagcttata tatgatcaaa
     7801 tactccttgt ataaataatg agaacaaaat ggaacttaaa aaattgatgg aacatatttc
     7861 tattattccc gattacagac aagcctggaa agtggaacat aaattatcgg atattctact
     7921 gttgactatt tg
//
LOCUS       DAZUFB010000163         7843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZUFB010000163 DAZUFB010000000
VERSION     DAZUFB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.163"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            178..396
                     /locus_tag="ACHY3M_004590"
     CDS             178..396
                     /locus_tag="ACHY3M_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS4319357.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            615..857
                     /gene="yiiF"
                     /locus_tag="ACHY3M_004591"
     CDS             615..857
                     /gene="yiiF"
                     /locus_tag="ACHY3M_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFS4319358.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1188..2117)
                     /gene="fdhE"
                     /locus_tag="ACHY3M_004592"
     CDS             complement(1188..2117)
                     /gene="fdhE"
                     /locus_tag="ACHY3M_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFS4319359.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQEQEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2114..2749)
                     /gene="fdoI"
                     /locus_tag="ACHY3M_004593"
     CDS             complement(2114..2749)
                     /gene="fdoI"
                     /locus_tag="ACHY3M_004593"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS4319360.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2746..3648)
                     /gene="fdoH"
                     /locus_tag="ACHY3M_004594"
     CDS             complement(2746..3648)
                     /gene="fdoH"
                     /locus_tag="ACHY3M_004594"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFS4319361.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPEARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3661..6711)
                     /gene="fdnG"
                     /locus_tag="ACHY3M_004595"
     CDS             complement(3661..6711)
                     /gene="fdnG"
                     /locus_tag="ACHY3M_004595"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6124..6126),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS4319362.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6905..7738
                     /gene="fdhD"
                     /locus_tag="ACHY3M_004596"
     CDS             6905..7738
                     /gene="fdhD"
                     /locus_tag="ACHY3M_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFS4319363.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1819 a         2013 c         2081 g         1930 t
ORIGIN      
        1 cctgggcgat aactaaacca tttttgagta ataaatgaaa caactgcaac actcttatta
       61 tttctggaaa acgcctcgca aaaacaaaac aacatgcaaa aatccgcttt ttttaaatga
      121 ttttccggcg taattttcgc acatacatta atgagtaata taggtaaggt ggtggtaatg
      181 gcgatgaata cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaaactt
      241 cggggctggc gtaaggtttc acgatctgca attttacgcg aagcggttga gcaatatctg
      301 gagcggcagc aatttccggt gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt
      361 ggtgttgatg atcagtgtaa ggagcataag gaatgaaaaa aatttttcat tcttgaatat
      421 aaaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg aacttataat
      481 aactgcaact gttacatcgt atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa
      541 atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc aaaaatgata
      601 ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt ttactcgatt
      661 tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg
      721 cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa
      781 ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc
      841 tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt
      901 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat
      961 atattgaagt ttattggtag atactgataa gcgcagcgca tcaggcaatc atacgcttgt
     1021 catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag gcaaacagac
     1081 aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc gtcacaccct
     1141 atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta ctccccttca
     1201 cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc cattcgcgcg
     1261 tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc tttttcctga
     1321 taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat cgcggcctgt
     1381 tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct gcattttacg
     1441 cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag accctgagtg
     1501 gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca atattgacgt
     1561 tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg cgcccagtag
     1621 agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga gaaatcagag
     1681 gcaaacagtg cgctggccat atcttcctgc tcctgagtcg atgccttctc cagattctca
     1741 atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag cgccatcagc
     1801 agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg cttgccctgt
     1861 gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc gtacagcacc
     1921 acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag cggattattt
     1981 tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag ccgagggaac
     2041 aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc atcttgcggg
     2101 attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca cggtaccagc
     2161 gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg gcggtaatcg
     2221 tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt aacgccactg
     2281 cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa
     2341 aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa ataatcgccg
     2401 cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact tcctcgttga
     2461 cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt aggttgtgat
     2521 gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg acaaacgggt
     2581 gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg aaggacggga
     2641 acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg atccagtggt
     2701 tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt tgcgctcctc
     2761 gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac cgacacccac
     2821 gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga gcggtttcca
     2881 gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca agccatgata
     2941 cagatttggc ttgtcagcat ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc
     3001 cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct
     3061 cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg ggcaggtctt
     3121 cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac atttgtagac
     3181 gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc
     3241 gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact gaataattgc
     3301 accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac agccgtcttt
     3361 gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca
     3421 cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc cgacggtatc
     3481 gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga tacaggtggt
     3541 gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctcaggcg cgggggtcag
     3601 accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat gtcgtctccg
     3661 ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc
     3721 accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc cttcatagcc
     3781 ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca gtgtacgaat
     3841 acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga ctttcacggt
     3901 atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca caaactgctc
     3961 tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat gctcggtcag
     4021 acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt cggcgtcgtc
     4081 tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt tagttcccag
     4141 cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct tatcgagggc
     4201 aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc tacccggagg
     4261 cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc atttcagcaa
     4321 ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt tatacagaat
     4381 gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc cagacggatc
     4441 ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca
     4501 gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa gttgttggcc
     4561 tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg ccttaccgtt
     4621 gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat
     4681 gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag
     4741 gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt
     4801 ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt ctgcgaagca
     4861 ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt cgttcagctc
     4921 accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg tgacgaggaa
     4981 cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg caacagggtt
     5041 aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga agtattgcag
     5101 gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat tttccgccgt
     5161 cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt agttgcccca
     5221 gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag
     5281 atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg acagtagccc
     5341 caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc cgccgccggc
     5401 catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt tttgcgcacc
     5461 aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat cgtgagcact
     5521 ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac cacagatgtt
     5581 ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc acacgcagcg
     5641 cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt cataagtcca
     5701 ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa
     5761 gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt attcgcggtt
     5821 gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc
     5881 ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc gaggatcgat
     5941 cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga acccgaccgg
     6001 gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct tgatgtcgac
     6061 ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta ccgttggtcc
     6121 gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg agaatttttg
     6181 cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg tggagagcca
     6241 gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt
     6301 catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc atttatcaga
     6361 acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga aatccaccag
     6421 gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat cgccttcgat
     6481 atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca tcaacagccc
     6541 acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca gcagtttata
     6601 ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc
     6661 tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca tggattgctc
     6721 cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca gggagtggga
     6781 gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt tccctccatc
     6841 caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat ctggttaaga
     6901 gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca caggtgttcg
     6961 tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg aggtcgcgga
     7021 agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga tggcgtcgcc
     7081 caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa tagaaagtcc
     7141 gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga
     7201 gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac
     7261 gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg tgcagccgct
     7321 accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc atctcaacga
     7381 ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga tgttgccatc
     7441 tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgttgg acaaactgtt
     7501 aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg tttccagtcg
     7561 tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt
     7621 gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga cgctggtggg
     7681 tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga gcaattaatt
     7741 aaaataagtc ctgcgatata tattaaattg caggacttat tcatttcgtg aattttatta
     7801 ttttatttat aaataaaacg cgtgtagtgg cgttttcttt ttt
//
LOCUS       DAZUFB010000164         7652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZUFB010000164 DAZUFB010000000
VERSION     DAZUFB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.164"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..351
                     /locus_tag="ACHY3M_004597"
     CDS             <1..351
                     /locus_tag="ACHY3M_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319364.1"
                     /translation="GAFLLRFKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLED
                     IMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLK
                     SKATAPIQNGPLPA"
     gene            complement(355..1746)
                     /gene="tcyP"
                     /locus_tag="ACHY3M_004598"
     CDS             complement(355..1746)
                     /gene="tcyP"
                     /locus_tag="ACHY3M_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFS4319365.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1879..2469)
                     /gene="ydjM"
                     /locus_tag="ACHY3M_004599"
     CDS             complement(1879..2469)
                     /gene="ydjM"
                     /locus_tag="ACHY3M_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4319366.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2632..3300)
                     /gene="hxpB"
                     /locus_tag="ACHY3M_004600"
     CDS             complement(2632..3300)
                     /gene="hxpB"
                     /locus_tag="ACHY3M_004600"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFS4319367.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3447..3983
                     /gene="yniB"
                     /locus_tag="ACHY3M_004601"
     CDS             3447..3983
                     /gene="yniB"
                     /locus_tag="ACHY3M_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFS4319368.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4024..4884)
                     /gene="yniA"
                     /locus_tag="ACHY3M_004602"
     CDS             complement(4024..4884)
                     /gene="yniA"
                     /locus_tag="ACHY3M_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4319369.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4990..5280)
                     /gene="ghoS"
                     /locus_tag="ACHY3M_004603"
     CDS             complement(4990..5280)
                     /gene="ghoS"
                     /locus_tag="ACHY3M_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS4319370.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5381..6310)
                     /gene="pfkB"
                     /locus_tag="ACHY3M_004604"
     CDS             complement(5381..6310)
                     /gene="pfkB"
                     /locus_tag="ACHY3M_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFS4319371.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALDVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6597..7355
                     /gene="ydiY"
                     /locus_tag="ACHY3M_004605"
     CDS             6597..7355
                     /gene="ydiY"
                     /locus_tag="ACHY3M_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFS4319372.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7408..7515)
                     /gene="yniD"
                     /locus_tag="ACHY3M_004606"
     CDS             complement(7408..7515)
                     /gene="yniD"
                     /locus_tag="ACHY3M_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFS4319373.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1974 a         1876 c         1863 g         1939 t
ORIGIN      
        1 ggagcatttt tgctgcgatt caaaaaaaat gagagtctcc taaagtttcc ttcgataata
       61 tcaatgctta atatcaatct tctttttaat ccactaaatc ttccgggaat gggaagtggt
      121 gtgcttgaag atattatgag tattccagat agttcactta gaaaacgtct cgggtatgag
      181 gttttaagct tctcactaca ggcacattca ttgagccagg aatgtattga taagttggac
      241 atttttttcg ctgacgatct gtttaaatat gagtcagtat gcatcgccgc gatggaacat
      301 ttaaaaagta aagcaacggc ccctatacaa aacggaccgt tgccagcata agaattaacg
      361 gtgtgccagt tcggcgtcgt cttcgctatc cagaatggct ttatcggttt gcttcagcca
      421 ctggctggtc agcgtgccag ctgtcatcga gccactaacg tttagcgccg tacggcccat
      481 gtcgataagc ggttcaacgg agattaacag cgccaccagg gttactggca ggcccatcgc
      541 aggcagcaca atcagtgcgg cgaaagttgc accaccaccg acaccggcaa cgcctgcgga
      601 actaacggta acaataccga ccagcgtcgc aatccacatc gggtccagcg ggttaatgcc
      661 aaccgtaggc gcaaccatca ccgccagcat tgccggatac aaaccggcgc agccgttctg
      721 accaatggtt gcaccgaaag aggcggcgaa actggcgatg gattcaggaa cgcccagacg
      781 acgcgtttgt gcttccacat tcagtgggat agacgcagca ctggaacggc tggtaaaggc
      841 aaacgtcagc acaggccata ccttacggaa gtacttcagc ggactcacgc cattaatgcc
      901 cagcagaatg ccatgcactg caaacataat cagcagaccg aggtaggacg cgacaacgaa
      961 acttcccagt ttgatgatgt cttgcaggtt agaacctgca accactttgg tcattagtgc
     1021 cagaacgccg taaggggtca actgcatgac caggcgaacc agtttcatca cccagctttg
     1081 cagggtatcg atagcggtta agacgcgttc acctttcggc gcatcatcct tcagcagttt
     1141 cagcgcagct acaccgagga atgcggcaaa aattaccacg ctgataattg acgtcggatt
     1201 ggctccggtc agatcggcaa acgggttttt cgggataaag gacaagacca gctgcggaac
     1261 gctcaggtca gagactttac caacatagtt actttcgatg gcgttcagac gtgcagtttc
     1321 tgcaccaccc tgaaccagac cttcagccgt caaaccaaac aggttggtga ccagcacacc
     1381 gaccagcgcc gcaatcagtg tggtaaacaa aagcgtaccg atggtcagaa aactgatttt
     1441 gcctaactga gatgcgttat gcagacgggc aaccgcgctc agaatagagg cgaagactaa
     1501 cggcataacg atcatttgca gcagttgaac atagccgtta ccaacgatgt taaaccactg
     1561 tacagaatct ttaagtacct ggctgtcaga accataaatg gtatgcaggg caaggccaaa
     1621 aaccacaccc atcaccagac ccaccagcac ttttttcgcc agactccact gtttgtggcg
     1681 ggtctgagcc agcgcaaaca gcagtacaac gaacaccacg atgttcgcaa ttaatggaaa
     1741 gttcatcccc gttctcctga tcttattatt tctatcactg cgtagcagtc atagtgttgg
     1801 cgcaagatta acagaactgt gagctggctc ttatattcaa atggaattgc ttattccaga
     1861 aaggtgattt ttgccttttt agtattcaac ctgatgctta ataagccgat gaaactggat
     1921 ctgcaaggta ttgatcattt gcgatgacca acgaaccgcg ctgttttcgg gtaatgaatg
     1981 gggcatccat accgaccaga caaataatgc catgcagata aaacgttcca gttggttgcc
     2041 cttttgggga accaggatag gcaagcggaa acgccagcga catggccaga gcaggggaac
     2101 accggcgggt gtcagcatat cggcaagtat gtggcttaaa taacccagca ccattccttg
     2161 tagcgcatca gccggaatga accagctttc aggaacctta aggtaaaagg ttgccagcag
     2221 cgcaaatacc gccagcagac tgtgggtaaa accacgatgc ccaaaagcgc gggcgatcgg
     2281 ttttgatatc cactttaatc gctgcccaag aaacgacttt gggtgatcga tgtccggtaa
     2341 caaacacgtc aggattgcgg aagggacaat atgccaccag tcaccctgtg ccagcacggg
     2401 cgtcagctcg gcatttttgg caaataccgc acaagcaata gaaaagagaa ggtgaccttc
     2461 cgccgtcatg ataaaactca ctaaactgtc gatacgtaca gtataggatt tttatacagt
     2521 gcacggaagt ggtgacggtg taactctttc tcttaaattg acgattaatc cgtcagttga
     2581 atgcaaattc agataaagaa ccaggggcag cgagatcact gcccctggtc attaaccgag
     2641 aaggtctttt gcggtgagtt ctgtcagcga tgagagtttg acgtttgcta atacaaaacg
     2701 tggatcattt tgcgcttctg gcgcaggaac gacaatggaa cgcatgcggg ctgctttaga
     2761 ggcgatcatg ccatttactg aatcttccag cgctacgcag gtcagtgggt caacgcccag
     2821 ttttgctgcg cagtcgagat atacttgcgg atgcggcttg ctgtaaggca gtttttcagc
     2881 cgaggcgagg gcatcgaaac tgtcgcgtaa gtcaaacatg gtcaacactt tttccagcat
     2941 atgtagtggt gacgcggagg ccagtcccac caataaacct tgttctttgc ataacgcaac
     3001 ggcttcgcgc acgcctggta ataatggacg tgtctcttca accagtgaaa tggcacgggc
     3061 aataacccgt tctactactt cctgacggct tggcccattc cacggttgcc gggcgtacca
     3121 aagatcgacc accatatcga tgcgtaaacc taaggtgtcc ggcagctcgt tacgacggga
     3181 gatatccacc cccaggcttg ccatcacatc cagttcggct cgatcccata aaggttctga
     3241 gtcgataagt aatccatcca tatcaaaaat tgcagcaaga atctgacgcg gggttgacat
     3301 tacaacctct cctttagagc attatgtgta aggtattaag ccgtgcttat gattaagcgc
     3361 ctatcatatc gcctttaaag aatgggcgaa aacagatatc tacaggtaaa ctaagtgaaa
     3421 cgacgcgtct taatcaagga gaactcatga cgtatcaaca agctggacgc attgctgttt
     3481 tgaaaaggat tttgggttgg gtgattttta tacctgcatt gatctctaca ttgatttctt
     3541 tgctgaaatt tatgaatacc cggcaggaaa accaggaagg cattaatgcg gtcatgctcg
     3601 actttactca tgtcatgatc gatatgatgc aggcgaatac gccttttttg aatctgttct
     3661 ggtataactc cccgacgccc aattttaatg gtggcgtgaa cgttatgttc tgggtgattt
     3721 tcatcctgat ttttgtcgga ctggcgttgc aggattccgg tgcccggatg agtcgccagg
     3781 cgcgcttctt gcgggaaggt gttgaagatc aactcattct ggaaaaagcc aaaggggaag
     3841 aggggctaac gcgtgaacaa attgagtccc gtattgttgt tccgcaccat actattttcc
     3901 tgcagttttt ctcactatat atcctgccgg ttatttgtat tgctgcaggt tatgtgttct
     3961 tttctctgct tgggtttatt taatcaaaag gccgcacgga gcggccatta tcctcaaccc
     4021 atatcatgct gctaataatc tatccaatga ctgctgagca ataaccaaat gctgaccgcc
     4081 aaataatctt gcacgattta acagcgtgta gagttggtaa accggttgac gttcaaggaa
     4141 atcggcaggt agcggggata ctgactgata gccgtcatag atttgtggcg gctgttcagt
     4201 atgcagcggt aacatcgcca ggtcgcactc tcggtcaccc cagtagcagg cagggtcgaa
     4261 aatgtacggg ccatccggac ccagcgcaca gttgccggac cataaatcgc cgtgcaacag
     4321 agaaggctgc ggttgatgcg aggccagacg ctgctgaata tgctcgacga tggcgtcgat
     4381 attgccgaaa gcgatccctt tctctgctgc cagttccaac tgccagccaa tccgttgttc
     4441 agcaaaaaac gttgaccagc gacgttgcca ggtgttgggc tgtggagttg tggagagcga
     4501 gttatcaaaa tcgaggccaa attgtggttg gtcactccat tgatgtaaac gcgcaatttg
     4561 ctgaccaaga ataaatgcgc tatgcgcatc cagcggacga ggtgggagat aatccatcac
     4621 cagaaaactg tagtcacggt cagcgcctac tgcccaaacc ttaggcacgg tgacggtttt
     4681 actacgcgac agtaactcca gttggtcggc ttcggcggta aaaccgggaa gcagttccct
     4741 ttcatcacat ttgacgaaaa agtcatgtcc tgcatagcgc aaatgccatg cggcatggac
     4801 ttctccgcca ggcagttcat tacgcagttc gatttcgcct tcacctaact gctcgctcaa
     4861 aagacgactg attgcctgcc acatgatgtc tctcccatgt tgtctgccag atcataaggt
     4921 tagcgcaaga atgcggtgta aaaacacgag ctaacgcaca ccacagactg attattgccc
     4981 aaaaaccatt tattttctgt tgctattcca ttcctcccag gtcgtgatgg tgatttcagg
     5041 atctttgccg gttgcacttt gggtaagccc cgaaaccagt tctctaattt cttcttcact
     5101 ttgggtactg ataagtccaa aagtgttagt ccccagctca tggatatttc cctcatcatc
     5161 cgtcatggtg agcaaaaaac cgtcgcgagt caggtaatta ttcaactcgt taatttcagt
     5221 caatgtatcc tcatgcaaca tgacggttat gacataacgg acaagatcgc cactggccat
     5281 agttcacccc tttgttatca catgactttt gagcatagtc ggagaaacgc gttgccgaca
     5341 ggttggtggt gattccccca atgctggggg aatgtttttg ttagcgggaa aggtaagcgt
     5401 aaattttttg cgtatcgtca tgggagcaca gacgtgttcc ctgattgagt gtggctgcac
     5461 tccccgcagc tacgccaaaa cggaccatct cttcaagaga ggcattttct gccagtttca
     5521 gtgtcatcgc gccgaccatg ctgtcaccag cgccaacggt actctggctt ttcaccggtg
     5581 gtggcaccac ctgaatgcag ttttcactat caacatccag cgctccttgt ggaccaaggg
     5641 aaacgacaac ccgtttggcc ttgccgctat taacgatttc ctgcgcggct ttgcggacat
     5701 cgtctggctg ggtgagttcg cgattcacca gcgcactgag ttctttttgg ttaggcttaa
     5761 ccaactcgat gttaccaatc gccagtgctg cacttaacgc ttcgccagaa ctgtcgatga
     5821 tgcagcggat cccttggttt tgcgcagcgg aaatcagttg ggttaatttt tccagcttca
     5881 cacctggcgg caggcttccg cttatgacca ggatggcccc ggattcaatt tccagaactt
     5941 gctcttcaag ctggcgaaac tcatcttcat ttaatgccgc gcctggcata acaaaacgat
     6001 actgctcacc gcttgcttcc acatgtacgt gtaaattctg ccgggtccag tctttggctt
     6061 ctacagtagc gacggggaca ttttcatccg ccaacagaga aaccaggtgt tcgccggtcg
     6121 cgccacccgc cgggaagatc gctgtggcac tgcctccaag atgggcaatg gcgcgggcga
     6181 cgttgatgcc gccgcccccg ggttcgaaca ccggtgcggt acagcgcagt tttccttcgg
     6241 gataaatttg cggggtaatt gttgcgctat cgagagaggg cgcaagtgtc aacgtataga
     6301 tacgtaccat catttcctcc tataggctga tttcagtctg gcaccgaatc agccgaaagt
     6361 gaagaaatta acaatatgat ttattagagc tttatttaaa attttgcaga taaatatata
     6421 taaataaaaa tctgttttcg ccacatgctt attcaaaaaa tgaaataaga atacattgct
     6481 aagttattcg cgccttttta taattcgtta cctttccagg gacgctaatc tttgatcctt
     6541 aagaaagttt ccgggaccgc gatggtctct ttttttgttg tacggactct tgataaatga
     6601 agcttttgaa gacagttcct gccatcgtta tgttggcggg gggcatgttt gcgtcactga
     6661 atgcagctgc cgatgattcc gtttttactg tcatggacga tcctgccagc gctaaaaaac
     6721 ctttcgaagg taatctgaac gcaggatatc ttgcacaatc aggcaatacg aaaagctcct
     6781 cacttacggc tgacaccacc atgacctggt atggacaaac cactgcctgg tcgctgtggg
     6841 ggaacgccag taatacttct tctaatgatg agcgttcttc agaaaaatat gcggcgggcg
     6901 ggcgtagccg ttttaattta actgattatg actatttatt tggtcaggca agctggctga
     6961 cagaccgtta taacggctat cgagagcgcg atgtgttgac cgcgggttat ggtcatcagt
     7021 ttttgaatgg cccggtacac agtttccgct ttgaattcgg tccaggtgtg cgttacgaca
     7081 aatataccga caatgccagt gaaacccagc cgctgggcta tgcctccggg gcttatgcat
     7141 ggcagttgac tgacaacgct aaatttacgc agggtgtttc ggtctttggc gcggaagaca
     7201 caacgctgaa ctccgaaagc gcattaaatg ttgctattaa tgaacatttt ggactgaagg
     7261 tggcttacaa cgtcacatgg aactctgaac ctccagagtc tgcaccggaa cataccgacc
     7321 gtcggacaac cttatcgctg ggctacagca tgtaaaatta tctgccggac cgtgttatgc
     7381 ggtctggctg tcttcgtttg tcactgctca gccccaaatc attgctgccc aacgtagcaa
     7441 caacatagcg ccacagattg ccagtagcac caccaccatc ttccagtgtt ttttatcaaa
     7501 gcgtttagtc ggcataccag tgtcctgcat atctcttgtg cgtgataacg gagtgtaaca
     7561 aaagggacgg gcatcaaggg attttttgtt attacagccg ttgggtggcg ttgtacacct
     7621 ggtcactgtt tctgcgtttt atgaaaaaaa ta
//
LOCUS       DAZUFB010000165         7603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZUFB010000165 DAZUFB010000000
VERSION     DAZUFB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.165"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..1192
                     /gene="mltB"
                     /locus_tag="ACHY3M_004607"
     CDS             107..1192
                     /gene="mltB"
                     /locus_tag="ACHY3M_004607"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFS4319374.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            1337..1834
                     /gene="pncC"
                     /locus_tag="ACHY3M_004608"
     CDS             1337..1834
                     /gene="pncC"
                     /locus_tag="ACHY3M_004608"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFS4319375.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            1914..2975
                     /gene="recA"
                     /locus_tag="ACHY3M_004609"
     CDS             1914..2975
                     /gene="recA"
                     /locus_tag="ACHY3M_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4319376.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            3218..3718
                     /gene="recX"
                     /locus_tag="ACHY3M_004610"
     CDS             3218..3718
                     /gene="recX"
                     /locus_tag="ACHY3M_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4319377.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            3846..6476
                     /gene="alaS"
                     /locus_tag="ACHY3M_004611"
     CDS             3846..6476
                     /gene="alaS"
                     /locus_tag="ACHY3M_004611"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4319378.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            6711..6896
                     /gene="csrA"
                     /locus_tag="ACHY3M_004612"
     CDS             6711..6896
                     /gene="csrA"
                     /locus_tag="ACHY3M_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFS4319379.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            7211..7303
                     /locus_tag="ACHY3M_004613"
     tRNA            7211..7303
                     /locus_tag="ACHY3M_004613"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7245..7247,aa:Ser,seq:gct)
     gene            7307..7383
                     /locus_tag="ACHY3M_004614"
     tRNA            7307..7383
                     /locus_tag="ACHY3M_004614"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7341..7343,aa:Arg,seq:acg)
BASE COUNT         1860 a         1834 c         2137 g         1772 t
ORIGIN      
        1 gttgagttat gtaaagtccg tatcgggcag tgactaccgc ttccttgtgt ggggcgtgat
       61 gctttaccat acttgcccct ggttgaatct gttaaatgga cccctcatgt tcaagcgtcg
      121 ttatgtaaca ttgcttcccc tttttgtgtt gcttgccgcc tgtagcagca agccaaaacc
      181 tactgagact gaaacgacca ccggaacgcc gtctggcggc ttcctgcttg agccgcagca
      241 caatgtgatg cagatgggcg gcgatttcgc taataacccg aatgcccagc agttcatcga
      301 caaaatggtg aacaaacacg gtttcgatcg tcagcagttg caggaaattc tctcccaggc
      361 gaagcgtctg gattcggtac tgcggctgat ggataaccag gcaccaacca catcggtgaa
      421 accgccatca ggtccgaacg gcgcatggct ccgttatcgc aaaaaattta ttacgccgga
      481 caacgtgcag aacggtgtgg ttttctggaa tcagtatgaa gatgcgttga atcgcgcgtg
      541 gcaggtgtat ggcgtaccgc cggaaattat cgtcgggatt atcggcgttg aaacccgctg
      601 ggggcgcgtg atggggaaaa ctcgcatcct cgatgcgctg gcaacgctgt catttaacta
      661 cccacgccgc gcggagtatt tctctggcga gctggaaacc ttcctgttga tggcgcgcga
      721 cgagcaggac gatccgctca atctgaaagg ttcctttgcc ggggcgatgg gctacggaca
      781 gtttatgccg tcgtcttaca aacaatatgc ggtagatttc agcggcgacg ggcatatcaa
      841 cctgtgggat ccggttgatg cgatcggtag cgtggcgaac tatttcaaag cgcacggctg
      901 ggtgaaaggc gatcaggtcg cggtaatggc aaacggtcag gctccaggct tgccaaatgg
      961 cttcaaaacc aggtacagca tttcgcagct tgccacggca ggtttaacgc cacagcagcc
     1021 gctgggcaac catcaacaag ccagcctgct gcgtctggat gtcggtaccg gttaccagta
     1081 ctggtacggt ctgccgaact tctacaccat tacccgttat aaccacagca cccattacgc
     1141 aatggcggtc tggcagttag gacaagccgt ggcgctggcg cgcgtgcagt aactatttgc
     1201 aagagggggg cgtgagcttc cctctggcta atcagctttt ttctgaatcc tcctcgtaaa
     1261 attgcaacgc caacaccatc ttcctgacga aagtgctatc ttgtccggca taaatttttg
     1321 actgacagag gttgtgatga ctgacagtga actgatgcag ttaagtgaac aggttgggca
     1381 ggcgctgaaa gcccgtggcg caaccgtaac aactgccgag tcttgtaccg gtggttgggt
     1441 agcgaaagtg attaccgata ttgccggtag ctccgcctgg tttgaacgcg gatttgtcac
     1501 ctacagtaac gaagccaaag cgcagatgat cggcgtacgc gaagagacgc tggcgcagca
     1561 tggcgcggtg agtgaacccg tcgtggtgga aatggcgata ggtgcactga aagcggctcg
     1621 tgctgattat gccgtgtcta ttagtggtat cgccgggccg gatggcggca gtgaagagaa
     1681 gcctgtcggc accgtctggt ttgcttttgc cactgcccgc ggtgaaggca ttacccggcg
     1741 ggaatgcttc agcggtgacc gtgatgcggt gcgccgtcag gctactgcgt atgcattgca
     1801 gaccttgtgg caacaatttc tacaaaacac ttgatactgt atgagcatac agtataattg
     1861 cttcaacaga acatattgac tatccggtat tacccggcat gacaggagta aaaatggcta
     1921 tcgacgaaaa caaacagaaa gcgttggcgg cagcactggg ccagattgag aaacaatttg
     1981 gtaaaggctc catcatgcgc ctgggtgaag accgttccat ggatgtggaa accatctcta
     2041 ccggttcgct ttcactggat atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg
     2101 tcgaaatcta cggaccggaa tcttccggta aaaccacgct gacgctgcag gtgatcgccg
     2161 cagcgcagcg tgaaggtaaa acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa
     2221 tctacgcacg taaactgggc gtcgatatcg ataacctgct gtgctcccag ccggacaccg
     2281 gcgagcaggc actggaaatc tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg
     2341 tcgttgactc cgtggcggca ctgacgccga aagcggaaat cgaaggcgaa atcggcgact
     2401 ctcacatggg ccttgcggca cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc
     2461 tgaagcagtc caacacgctg ctgatcttca tcaaccagat ccgtatgaaa attggtgtga
     2521 tgttcggtaa cccggaaact actaccggtg gtaacgcgct gaaattctac gcctctgttc
     2581 gtctcgacat ccgtcgtatc ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa
     2641 cccgcgtgaa agtggtgaag aacaaaatcg ctgcaccgtt taaacaggct gaatttcaga
     2701 tcctctacgg cgaaggtatc aacttctacg gcgaactggt tgacctgggc gtaaaagaga
     2761 agctgatcga gaaagcaggc gcgtggtaca gctacaaagg tgagaagatc ggtcagggta
     2821 aagcgaatgc aactgcctgg ctgaaagata acccggaaac cgcgaaagag atcgagaaga
     2881 aagtacgtga gttgctgctg agcaacccga actcaacgcc ggatttctct gtagatgaca
     2941 gcgaaggcgt agcagaaact aacgaagatt tttaatcgtt cgttattgat gataatagat
     3001 aatgtgtgca cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggaacag
     3061 gccagcactg gcgtgaaaaa ttactctcac ctcgatcctc taacgaattg ggcctgcggg
     3121 aacattctgc acactcattg actattaaat ctgtttatca gcaaaacctg ggtcgcatct
     3181 gcggcccttt tgctttttta agttgtaagg atatgccatg acagaatcaa cgtcccgtcg
     3241 cccggcatat gctcgcctgt tggatcgtgc ggtacgcatt ctggcggtgc gcgatcacag
     3301 tgagcaagaa ctgcgacgta aactcgtggc accgattatg ggcaaaaatg gcccagaaga
     3361 gattgatgct acggcagaag attacgagcg cgttattgcc tggtgccatg aacatggcta
     3421 tctcgatgac agccgatttg ttgcgcgctt tatcgccagc cgtagccgca aaggttatgg
     3481 acctgcacgt attcgtcagg aactgaatca gaaaggtatt tcccgcgaag cgacagaaaa
     3541 agcgatgcgt gaatgtgaca tcgactggtg cgcactggcg cgcgatcagg cgacgcgaaa
     3601 atatggtgaa cctttgccaa ctgtcttttc agaaaaagtt aagatccagc gttttctgct
     3661 ctatcgtggc tatctgatgg aagatatcca ggatatttgg cgaaattttg ccgactgaac
     3721 gcatacggta ttttacttcc cagtcaagaa aacttatctt attcccactt ttcagttacc
     3781 agcccggcgg ttaagacacg ctggagctgg tggcgatatt tcgttagctt gatttcagga
     3841 taattatgag caagagcacc gctgagatcc gtcaggcgtt tctcgacttt ttccatagta
     3901 agggacatca ggtagttgcc agcagctccc tggtacccca taacgaccca actttgttgt
     3961 ttaccaacgc cgggatgaac cagttcaagg atgtgttcct tgggctcgac aagcgtaatt
     4021 attcccgcgc taccacttcc caacgctgcg tgcgtgcggg tggtaaacac aacgacctgg
     4081 aaaacgtcgg ttacaccgcg cgtcaccata ccttcttcga aatgctgggc aacttcagct
     4141 tcggcgacta tttcaaacac gatgccattc agtttgcatg ggaactgctg accagcgaaa
     4201 aatggtttgc cctgccgaaa gagcgtctgt gggttaccgt ctatgaaagc gacgacgaag
     4261 cctacgaaat ctgggaaaaa gaagtcggga tcccgcgcga acgtattatt cgcatcggcg
     4321 ataacaaagg tgcgccatac gcatctgaca acttctggca gatgggtgac actggtccgt
     4381 gcggcccgtg caccgaaatc ttctacgatc acggcgacca catttggggt ggtcctccgg
     4441 gaagtccgga agaagacggc gaccgctaca ttgagatctg gaacatcgtc ttcatgcagt
     4501 tcaaccgcca ggcagatggc acgatggaac cgctgccgaa gccgtctgta gataccggta
     4561 tgggtctgga gcgtattgct gcggtgctgc aacacgttaa ctctaactat gacatcgacc
     4621 tgttccgcac gttgatccag gcggtagcga aagtcactgg cgcgaccgat ctgagcaata
     4681 aatcgctgcg cgtaatcgct gaccacattc gttcttgtgc gttcctgatc gcggatggcg
     4741 taatgccgtc caatgaaaac cgtggttatg tactgcgtcg tatcattcgt cgcgcagtgc
     4801 gtcacggcaa tatgctcggc gcgaaagaaa ccttcttcta caaactggtt ggtccgctga
     4861 tcgacgttat gggctctgcg ggtgaagacc tgaaacgcca gcaggcgcag gttgagcagg
     4921 tgctgaagac tgaagaagag cagtttgccc gtactctgga gcgcggtctg gcgttgctgg
     4981 atgaagagct ggcaaaactt tctggtgata cgctggatgg tgaaacggct ttccgtctgt
     5041 acgacaccta tggcttcccg gttgacctga cggctgatgt ttgtcgtgag cgcaacatca
     5101 aagttgacga agctggtttt gaagcagcaa tggaagagca gcgtcgtcgc gcgcgcgaag
     5161 ccagcggctt tggtgccgat tacaacgcaa tgatccgtgt tgatagtgca tctgaattta
     5221 aaggctatga ccatctggaa ctgaacggca aagtgaccgc gctgtttgtt gatggtaaag
     5281 cggttgatgc aatcaatgca ggccaggaag ctgtggtcgt gctggatcaa acaccattct
     5341 atgcggaatc cggcggtcag gttggtgata aaggcgaact gaaaggcgct aacttctcct
     5401 tcgcggtgga agatacgcag aaatacggcc aggcgattgg tcacatcggt aaacttgctg
     5461 cgggttctct gaaagtgggc gacgctgtgc aggctgatgt tgatgaggct cgtcgcgccc
     5521 gtattcgtct gaatcactcc gcaacgcacc tgatgcacgc tgcgctgcgc caggttctgg
     5581 ggactcatgt atcgcagaaa ggttcactgg ttaacgacaa ggtgctgcgc ttcgacttct
     5641 cacacaacga agcgatgaaa ccggaagaga ttcgtgcggt cgaagacctg gtgaacgcac
     5701 agattcgtcg caatttgccg atcgaaacca acatcatgga tctcgaagcg gcgaaagcga
     5761 aaggtgcgat ggcgctgttc ggcgagaagt atgatgagcg tgtacgcgtg ctgagcatgg
     5821 gcgatttctc caccgagctg tgtggcggta ctcacgccag ccgcactggt gatattggtc
     5881 tgttccgcat catctctgaa tcgggtactg ctgcaggcgt tcgtcgtatc gaagcggtaa
     5941 ccggagaagg cgctatcacc accgttcatg cagacagcga tcgcttaagc gaagtcgcgc
     6001 atctgctgaa aggcgatagc aataatctgg cggataaagt gcgctcagta ctggaacgta
     6061 cgcgtcagtt agaaaaagaa ctacaacagc ttaaagaaca agctgccgca caggagagcg
     6121 caaatctttc cagtaaggca attgatgtta atggtgttaa gctgttggtt agcgagctta
     6181 gcggtgttga gccgaaaatg ttgcgtacca tggttgacga tttaaaaaat cagctggggt
     6241 cgacaattat cgtgctggca acggtagccg aaggtaaggt ttctctgatt gcaggcgtat
     6301 ctaaggacgt cacagatcgt gtgaaagcag gggaactgat tggtatggtc gctcagcagg
     6361 tgggcggcaa gggtggtgga cgtcctgaca tggcgcaagc cggtggtacg gatgctgcgg
     6421 ccttacctgc agcgttagcc agtgtgaaag gctgggtcag cgcgaaattg caataatata
     6481 agcgtcaggc aatgccgtgg actcgcttca cggcattcgc attaacgcta tcgacaacga
     6541 taaagtcagg ttgaagttgt gtatatcggc taaacttagg tttaacagaa tgtaatgcca
     6601 tgactgctta catgtaatgt gtttgtcatt gcttactttt tggcgttata tgatggataa
     6661 tgccgggata cagagagacc cgactctttt aatctttcaa ggagcaaaga atgctgattc
     6721 tgactcgtcg agttggtgag accctcatga ttggggatga ggtcaccgtg acagttttag
     6781 gggtaaaggg caaccaggta cgtattggcg taaatgcccc gaaggaagtt tctgttcacc
     6841 gtgaagagat ctaccagcgt atccaggctg aaaaatccca gcagtccagt tactaatctt
     6901 tccgcgtctc atctttatcg gtgagacgca ccctcaaaat ttctccttca ctctatagtc
     6961 ttttcgcttt actcccgttc attcaactta agtctccatt ttttgcatta ctactatctg
     7021 tcagacctcc atttttctgt tgataaaaca ctctttttga cgtttttaca gactaattga
     7081 acgtgaagtg tgcaaacgat aaaagtgtag gaaaaattgt ttgacttata agtctcagaa
     7141 agtaatatgt gcgccacgca gcgacgatga gcgataaaca agttcttcga agcactcgta
     7201 agaggcgtgt ggtgaggtgg ccgagaggct gaaggcgctc ccctgctaag ggagtatgcg
     7261 gtcaaaagct gcatccgggg ttcgaatccc cgcctcaccg ccatttgcat ccgtagctca
     7321 gctggataga gtactcggct acgaaccgag cggtcggagg ttcgaatcct cccggatgca
     7381 ccatattcta cgtactttca gcgatgaagg tatggaagag gtggcggtat aaccgcaggc
     7441 accagggagg ataacgttgc tttagcaacg gcccgaaggg cgagccgcaa ggcgagtaat
     7501 cctcccggat gcaccatctc ttacttgata cggctttagt agcggtatca aaaaatctgc
     7561 agtaaagtaa gtttcccgat gcatccgtag ctcagctgga tag
//
LOCUS       DAZUFB010000166         7542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZUFB010000166 DAZUFB010000000
VERSION     DAZUFB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.166"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..1327
                     /gene="acrE"
                     /locus_tag="ACHY3M_004615"
     CDS             170..1327
                     /gene="acrE"
                     /locus_tag="ACHY3M_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFS4319380.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1339..4443
                     /gene="acrF"
                     /locus_tag="ACHY3M_004616"
     CDS             1339..4443
                     /gene="acrF"
                     /locus_tag="ACHY3M_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFS4319381.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLEHYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4696..4917
                     /locus_tag="ACHY3M_004617"
     CDS             4696..4917
                     /locus_tag="ACHY3M_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319382.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5348..6373
                     /locus_tag="ACHY3M_004618"
     CDS             5348..6373
                     /locus_tag="ACHY3M_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4319383.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6441..>7542
                     /locus_tag="ACHY3M_004619"
     CDS             6441..>7542
                     /locus_tag="ACHY3M_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4319384.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTM"
BASE COUNT         1793 a         1827 c         1926 g         1996 t
ORIGIN      
        1 atattaatct ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta
       61 catcgacgaa tgataatttg taggatagca aactgtattt ttctttctgc gagttaacgc
      121 gttgcctttc tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      181 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      241 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      301 gaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      361 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgtaatttca ctgaaggcag
      421 cgatgtacaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      481 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      541 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      601 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      661 aagcgctcgc atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      721 caaatcgacc gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      781 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      841 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      901 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt
      961 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1021 tacgcttttg ccgggtatgt ttgtgcgtgc acggattgat gaaggcgtcc aacctgacgc
     1081 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1141 tgttaacgat aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1201 taaatggttg attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1261 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1321 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1381 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1441 tatccaacca ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg
     1501 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac
     1561 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1621 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1681 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1741 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1801 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1861 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1921 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     1981 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt
     2041 attgctcaaa cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2101 agtgacggct cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2161 tataacgtta tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca
     2221 accggcgcga atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2281 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag
     2341 ctttctattc acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg
     2401 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2461 gtgttgttag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2521 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2581 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca
     2641 atgtcgcaaa tccagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt
     2701 ccgatggcat tcttcggcgg ttctactggg gcaatttatc gccagttctc tatcaccatc
     2761 gtttcggcaa tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca
     2821 acgctgctta aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg
     2881 tttaatacta ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc
     2941 ggttccacag gacgatattt actgatctat gcgctgattg ttgcaggaat ggtggtgttg
     3001 tttttacgtc ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgaccatg
     3061 attcagttac ccgctggcgc gacgcaagag cggacgcaaa aagtgttgga tcaagtgacg
     3121 gattactatc tgaagaacga gaaagcgaac gttgaaagtg tctttacggt taacggcttt
     3181 agcttcagcg gccaggcaca aaacgccggt atggccttcg tcagtctgaa accgtgggaa
     3241 gagcgtaatg gtgatgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattt
     3301 ggcaagatcc gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc
     3361 acggcaacgg gtttcgactt tgagttaatt gatcaggctg ggctgggtca cgatgcccta
     3421 acccaggccc gtaaccagtt gcttggtatg gcggcgcaac atcctgccag cttagtcagc
     3481 gtgcgtccta atggcctgga agacaccgcg cagtttaaac tggaagttga ccaggaaaag
     3541 gcgcaggcat taggtgtttc actttctgac atcaatcaga ccatttcaac ggcgttgggt
     3601 gggacttacg ttaacgactt catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg
     3661 gatgccaaat tccgtatgct gccagaagat gtcgataaac tttatgtccg cagcgccaac
     3721 ggcgaaatgg tgccattctc ggcctttacc acttcacatt gggtgtatgg ctctccgcga
     3781 ctggaacact acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc
     3841 agttccggcg atgccatggc gttgatggaa aaccttgcgt caaaattacc tgctggcatt
     3901 ggttatgact ggacgggtat gtcgtatcag gaacgcttat cgggaaacca ggctcccgct
     3961 ctggtagcaa tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg
     4021 tcaattcctg tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctgctggcg
     4081 gcgacactct ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt
     4141 ggcttgtcgg ccaaaaacgc tattttgatt gttgagttcg ctaaagatct catggagaaa
     4201 gagggtaaag gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc
     4261 ctgatgacct ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgcc
     4321 ggcagtggcg cgcagaacgc agtgggtatc ggggtaatgg gcggaatggt ctctgcaacg
     4381 ttgctggcaa tcttcttcgt accggtgttc tttgtggtga tccgccgttg ctttaaagga
     4441 taaatcagaa acataaaggc actttcgggt gcctttatta ttttcagtga aacccataaa
     4501 aattaataag atattcttcc actcactctt taaaaacttt ctatagttcc cgctcccttc
     4561 actattttta caattcacat aatttgtcat tattcattcc gcaatattta taacctccgg
     4621 tgataaaatg gcattcagct cgttaataag agagttaact tattaagcgt tagcgtttat
     4681 tactgaggta acaccatgaa aagattaatt cctgtggcat tgctcaccgc attgctggcg
     4741 ggctgtgctc acgattcgcc ctgcgttccg gtttatgacg atcagggtcg tctggttcac
     4801 accaatacct gtatgaaagg cacgactcag gataactggg aaacggctgg ggctattgcc
     4861 ggcggggcag cggctgttgc tggcctgacg atggggatca tcgctctgtc gaaataactg
     4921 aacagggcgc ggagtttcgc gctctgttta acgatacagt atctttacat aagataaaat
     4981 attgctatat tctgctggct aattctctgc tgatttagca tgttatatcg cgttatttcg
     5041 ctgctttcga aataacgcat caatttaact ttcacacttc ccaccagaaa tatactcacg
     5101 ctaacaatca ttattctgct tagtaaaaca cgcgtaaata ttttgcgcca aaatgtggcg
     5161 catgtttcat tttcgcacca ttgcggggcg ctgtttttat tatttcctgt ctacactcag
     5221 cttattgcga cgcgaaatcg ggcgcaaaag ctggcactac ttttgcttat aagaggatgg
     5281 ccacagacag gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg
     5341 aagcattatg aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt
     5401 tgcaaatcag gcgcatgctg gagcgacgct tgatgccgtt cagaaaaaag gttttgtgca
     5461 atgcgggatc agtgatggat tacctgggtt ctcttatgcc gatgcagacg gtaagttttc
     5521 aggtattgat gttgatgttt gtcgtggtgt tgccgctgct gtatttggtg acgacacgaa
     5581 agtgaaatat accccactca cagcaaaaga acgcttcacg gctttacagt caggggaagt
     5641 ggatttgctc tcccgtaata cgacctggac ttcatctcgc gacgccggga tgggaatggc
     5701 atttactggc gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct
     5761 aaaaagcgcg aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga
     5821 actcaacgtt gccgactatt tcaaggcaaa caatatgaag tacacaccgg tgactttcga
     5881 tcgctctgac gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga
     5941 tcagtcgcaa ctgtatgccc tgcgcatcaa attaagcaac ccagctgaat ggattgtctt
     6001 accggaggtt atctctaaag aaccgcttgg gccggtagtt cgtcgtggcg atgatgaatg
     6061 gttctcgatt gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa
     6121 ttcccagaac gtcgatgaaa aagcggctaa tccagcaacg cctgatatgg cacatctgct
     6181 gggtaaagaa ggcgattacg gcaaggatct gaagttggat aataaatggg cctataacat
     6241 catcaaacag gtgggtaact actcggaaat tttcgagcgt aatgtaggtt cagaaagccc
     6301 gctgaaaatt aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc
     6361 gcccgtgcgt taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg
     6421 ttatgctttc cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctcct
     6481 ttgccaaccc tacggttcgt gcctggttat tccagatcct tgccgttgtt gctgttgtcg
     6541 gcattgttgg ttggttattt cacaacactg taacgaatct cagtaatcgt ggcattactt
     6601 caggttttgc ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt
     6661 accagcaggg cgacacctac ggacgcgttt ttattgtcgg cttactcaat acgctactgg
     6721 tttctgcatt gtgtattgtg ttcgcttctg ttttgggctt ctttatcggt ctggcgagac
     6781 tttcggataa ctggctgcta cgaaagcttt ccacaattta tattgagatc ttccgtaata
     6841 ttcccccgct gctgcaaatc ttcttctggt actttgccgt gttgcgcaat ttgcccggac
     6901 cacgccaggc tgtgagcgcg tttgatctgg cctttttgag caatcgtggg ctttatattc
     6961 cgtcaccgca gctgggagac ggatttattg cgtttatcct ggctgttgtt atggctatag
     7021 tcctttctgt tgggctattc cgctttaata aaacatacca gataaagacc ggacaactgc
     7081 gccgcacctg gccgatcgcc gcagtgttga tcattggttt gcctttactg gcgcaatggc
     7141 tttttggcgc agcactacac tgggatgtcc cagccctacg aggctttaac ttccgcggcg
     7201 ggatggtttt aattcctgaa ctggcagcct taacgctggc actttcggtt tatacatctg
     7261 catttatcgc cgagattatc cgcgctggga tccaggcagt gccttatggt caacatgaag
     7321 cggctcggtc actgggatta cccaacccgg ttacgctacg ccaggtcatt attccccagg
     7381 cactgcgagt gattattccg ccgttaacca gccagtatct caacatcgtc aaaaactcct
     7441 ctcttgccgc cgctattggc tatcccgata tggtttcgct gtttgctggc accgtgctga
     7501 accagacggg gcaagccatc gagacgatag ccatgaccat gt
//
LOCUS       DAZUFB010000167         7525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZUFB010000167 DAZUFB010000000
VERSION     DAZUFB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.167"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(75..494)
                     /locus_tag="ACHY3M_004620"
     CDS             complement(75..494)
                     /locus_tag="ACHY3M_004620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4319385.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(515..1897)
                     /gene="atpD"
                     /locus_tag="ACHY3M_004621"
     CDS             complement(515..1897)
                     /gene="atpD"
                     /locus_tag="ACHY3M_004621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4319386.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(1924..2787)
                     /gene="atpG"
                     /locus_tag="ACHY3M_004622"
     CDS             complement(1924..2787)
                     /gene="atpG"
                     /locus_tag="ACHY3M_004622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4319387.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(2838..4379)
                     /gene="atpA"
                     /locus_tag="ACHY3M_004623"
     CDS             complement(2838..4379)
                     /gene="atpA"
                     /locus_tag="ACHY3M_004623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4319388.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(4392..4925)
                     /gene="atpH"
                     /locus_tag="ACHY3M_004624"
     CDS             complement(4392..4925)
                     /gene="atpH"
                     /locus_tag="ACHY3M_004624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4319389.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(4940..5410)
                     /gene="atpF"
                     /locus_tag="ACHY3M_004625"
     CDS             complement(4940..5410)
                     /gene="atpF"
                     /locus_tag="ACHY3M_004625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4319390.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(5472..5711)
                     /gene="atpE"
                     /locus_tag="ACHY3M_004626"
     CDS             complement(5472..5711)
                     /gene="atpE"
                     /locus_tag="ACHY3M_004626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFS4319391.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(5758..6573)
                     /gene="atpB"
                     /locus_tag="ACHY3M_004627"
     CDS             complement(5758..6573)
                     /gene="atpB"
                     /locus_tag="ACHY3M_004627"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4319392.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(6582..6962)
                     /gene="atpI"
                     /locus_tag="ACHY3M_004628"
     CDS             complement(6582..6962)
                     /gene="atpI"
                     /locus_tag="ACHY3M_004628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFS4319393.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         1827 a         2040 c         1853 g         1805 t
ORIGIN      
        1 ctcttcagga cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc
       61 ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc
      121 gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg
      181 tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga
      241 attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac
      301 agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg
      361 gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc
      421 tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg
      481 taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag
      541 cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt
      601 ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac
      661 cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac
      721 gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaggatg gcgatgatgt
      781 ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt
      841 cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg
      901 ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa
      961 aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatacc gcctgtacgg
     1021 aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg
     1081 ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg
     1141 taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt
     1201 cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg
     1261 gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt
     1321 cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac
     1381 cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac
     1441 ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa
     1501 ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg
     1561 cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac
     1621 ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt
     1681 cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac
     1741 gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt
     1801 gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta
     1861 cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc
     1921 tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg
     1981 acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc
     2041 ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg
     2101 ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg
     2161 ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg
     2221 cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat
     2281 gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac atcactttta ccggaccgat
     2341 cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc
     2401 cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc
     2461 tttgtcggtc caggtcttca tttccgccag cagttttttg aacaggttaa tgttcaaacc
     2521 accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcgttc
     2581 ttccaggtat gggtgcttat attccagatt accgctcgca aggtgaccaa tcactttgcg
     2641 catggtatct gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga
     2701 agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt
     2761 actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta
     2821 aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc
     2881 agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga
     2941 gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccgat tttcgacagt
     3001 tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg ttgcgctacg
     3061 gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc
     3121 tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga
     3181 tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct
     3241 gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg
     3301 aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg
     3361 aacgcagaaa cgtcacccgc ctgagtttca ataatcggca gtgcggtcag agaaccggtt
     3421 ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgcgcagca
     3481 cgctccagca gacgagagtg gaggtagaat acgtcgcccg ggaatgcttc acgtcctggc
     3541 ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg
     3601 taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca
     3661 tacggagcca gatattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg
     3721 ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc
     3781 tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg
     3841 atagccagtg cggttttacc ggtctgacgg tcaccgatga tcaattcacg ctgaccacga
     3901 ccaattggga tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac
     3961 tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc
     4021 ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg
     4081 ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca
     4141 gcgtacggac ccataacaac cgcacctaca gagtcgcgct cgaggttcag tgcgatagcg
     4201 taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg
     4261 cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca
     4321 ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg
     4381 ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg
     4441 ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt
     4501 ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg
     4561 agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc
     4621 acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt
     4681 tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg
     4741 ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt
     4801 tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg
     4861 tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca
     4921 gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag
     4981 cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa
     5041 cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct
     5101 gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg
     5161 agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct
     5221 ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa
     5281 ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt
     5341 acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt
     5401 taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa
     5461 agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc
     5521 gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga
     5581 tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca
     5641 gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc
     5701 aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta
     5761 atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg
     5821 cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca
     5881 ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa
     5941 cagtcgcaaa ccaagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt
     6001 gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc
     6061 gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca atgccataga
     6121 cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg
     6181 ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac
     6241 ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc
     6301 tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg
     6361 cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag
     6421 caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg
     6481 tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat
     6541 gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt
     6601 acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag
     6661 aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg
     6721 aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg
     6781 tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt
     6841 attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc
     6901 accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac
     6961 atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt
     7021 aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca
     7081 aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc
     7141 gacttttctg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactggtg
     7201 ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagcatatt tatttacttg
     7261 gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttttaata
     7321 aatgacattt acacaacaaa aaccacccat tgacattttt aataatgttt taacagccaa
     7381 tgatggttct tagcgccgat ttttagcaga ctgatatttt cactaatgac ttattttctg
     7441 cttaccaaaa aaagccacgt tatcttgttg atgcaaaaga gtgaacgtgg cgttaaatgt
     7501 aaccagttat atcagtagaa aacct
//
LOCUS       DAZUFB010000168         7457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZUFB010000168 DAZUFB010000000
VERSION     DAZUFB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.168"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHY3M_004629"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHY3M_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFS4319394.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQHALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHY3M_004630"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHY3M_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFS4319395.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHY3M_004631"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHY3M_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFS4319396.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHY3M_004632"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHY3M_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFS4319397.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHY3M_004633"
     CDS             7014..7292
                     /locus_tag="ACHY3M_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319398.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..>7457)
                     /gene="gadE"
                     /locus_tag="ACHY3M_004634"
     CDS             complement(7293..>7457)
                     /gene="gadE"
                     /locus_tag="ACHY3M_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFS4319399.1"
                     /translation="KIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSD
                     IVTLGITSYF"
BASE COUNT         1909 a         1764 c         1802 g         1982 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacatgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtatcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taatttt
//
LOCUS       DAZUFB010000169         7323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZUFB010000169 DAZUFB010000000
VERSION     DAZUFB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.169"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..279
                     /locus_tag="ACHY3M_004635"
     CDS             34..279
                     /locus_tag="ACHY3M_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319400.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            282..860
                     /locus_tag="ACHY3M_004636"
     CDS             282..860
                     /locus_tag="ACHY3M_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFS4319401.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTHEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYMSLKKGEAIEHVLNGDKWLYTT"
     gene            927..1082
                     /locus_tag="ACHY3M_004637"
     CDS             927..1082
                     /locus_tag="ACHY3M_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319402.1"
                     /translation="MSSKANYESLASIMPRNGQEADAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            1584..2210
                     /locus_tag="ACHY3M_004638"
     CDS             1584..2210
                     /locus_tag="ACHY3M_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319403.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            2207..2884
                     /locus_tag="ACHY3M_004639"
     CDS             2207..2884
                     /locus_tag="ACHY3M_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4319404.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILARALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQHYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            2881..3582
                     /locus_tag="ACHY3M_004640"
     CDS             2881..3582
                     /locus_tag="ACHY3M_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319405.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEDPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWIPLISSFFEQESE"
     gene            3884..5146
                     /locus_tag="ACHY3M_004641"
     CDS             3884..5146
                     /locus_tag="ACHY3M_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319406.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLEIEH
                     LKRQQQDAIEDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            5219..5725
                     /locus_tag="ACHY3M_004642"
     CDS             5219..5725
                     /locus_tag="ACHY3M_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HFS4319407.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLHQQ"
     gene            5920..6501
                     /locus_tag="ACHY3M_004643"
     CDS             5920..6501
                     /locus_tag="ACHY3M_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319408.1"
                     /translation="MKGEVKERGMIFNDEMVRAILGGNKTQTRRIVEEKFYGRAVAAE
                     LLAKHCPYGQPGDRIWVRETYRVHGKATDVATLVYRASVRNSWTEQTHRVPVEVCNKP
                     VSEKWTPSIHMPRWASRILLEITNVRVERLNDISECDARAEGAPTESTLIGDKHYPGF
                     RSLWKSIYGEDSWNANPWVWVIEFERIQGATSE"
     gene            6494..6760
                     /locus_tag="ACHY3M_004644"
     CDS             6494..6760
                     /locus_tag="ACHY3M_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319409.1"
                     /translation="MSESKCQINGNKIEPCAALAQSLEHDAEYTTRKGLLKYKIYNHE
                     LIHSQDLIMLLSGEFSKSPIRVSFCPFCGESLKTWEAEATSEQN"
     gene            6747..>7323
                     /locus_tag="ACHY3M_004645"
     CDS             6747..>7323
                     /locus_tag="ACHY3M_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4319410.1"
                     /translation="MSKINYQALREIAKQATQGEWGAFISTGTGTYAVHTPGDKRCED
                     VIKWTGFDGLKNAENNARYIAAFNPKVALELLGEIKRLEDTNIDAMCRIAPLETKLAA
                     LVAENAGLKHAMAVTLDHVSVTDAGQAGVAAMIINDALHHSETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTR"
BASE COUNT         2003 a         1599 c         1926 g         1795 t
ORIGIN      
        1 gcccttaagt aggttattca cgaggaaaca aaattggcag aacgtgttga tgatgcagag
       61 ctgagcatga atcagttaga agctctcaaa gacatggcca tcgataacat cagaaagcag
      121 gcacaggtcg tgagccaggt atttacaggg aagtgtcgtt actgcaatga atcgattgaa
      181 tcaggcattt attgtgacgc tgaatgtgcg caatggcaca gggaagagca ggccgcaaaa
      241 cagcgtaaat atggcatgcg accggcagga tttgactgat tatgttgcgc tttactgagg
      301 aagagtttca ggcttttagt gagcgtcgaa ataaggggcg gtccaggcca aaaaccaaaa
      361 aggatccatt cttatcgctt gcgccggtaa aagaagtttc tccacatgcg aaggcacttg
      421 cagcactggc aaagaaccca gacctgcgcg acggaaattg cgagcacttc gagcaggttt
      481 tcatttttga ttacttcgaa cgcaagcacc ctgacatcta tgagctgttg catgcaacgc
      541 ctaacggagg gaaacgttca aaagcaaccg ccgggaaaat gaaggctgaa gggcagaaaa
      601 aaggttatcc ggacatgagt ctcgataaag catgcggtat ttatcacggc atgcgaattg
      661 agcttaaaga accaaatggt aaagccccga cgcatgagca gatcgcctgg atgcgcaggc
      721 ttagagagga aggttactac gtcgttcttg cgtatggtgc agaacaagcg ataaccgcca
      781 tcctggaata catgagcctt aaaaagggtg aggctattga gcatgtattg aacggtgaca
      841 agtggttgta cactacgtga aataataaat taattagtgc atatgtattc tttgatacag
      901 cgcacattaa catcgggaga ataatcgtgt catccaaggc taattatgaa tcgctggcat
      961 cgatcatgcc gcgtaatgga caggaagcag atgctgtagt ggacccggta atcgctgaaa
     1021 tgaatgctcg cctggaggct gaatttgcag ctgagaatga acataccacc cagggcgact
     1081 aggactgttt tttgtgtcgg tagcggtccg tcactcactc gtgaggactg tgctgctata
     1141 gaaaaaactg gctgttcaat catcgcggtt aacaattcct ggcagatgtt cgatgacatt
     1201 tatgccttat acgccggtga tttgtcatgg tggaagcaat acggatccac cataccggga
     1261 gggagattcc gcaaagtgac agccaacctg gcggcggcga aatcattttc gttggagtac
     1321 aggcgatatt gtggaccggc agaaggggta aatagcggcg cgcaggctat cagtctggct
     1381 gctgaatcag gggctgaagt agtggtatta gtcggctatg actgttctct gcaaaacggc
     1441 cttcattggc atggcgcgca ccctcaagca ctacggaatc caacgcaggt gtctatttca
     1501 aaatggcaac agcagttcct ggatacccgc aaaaaacacg cagatttaca tattttgaat
     1561 gcaagtagga gcagtgcaat tcaatgtttc ccaagaataa atttagaggc agtgatcgcg
     1621 ttattatcgt cggcagtggc ccaagcgccg caaactttgt tgcgccgcgc ggagtgccga
     1681 ttatagcggt caatggggcc atcgactggc ttaaccgcgc ttcttatttt ttcacccttg
     1741 atccatcgcc agacaatatg cggcgcgttg gtcgtggccg ccgtcgccgt ggtgtttgtt
     1801 attgcatggc gctacccgat gttaaagaac gtgaggtcag agacggcgtt ctgtgcttcc
     1861 gtcgtgtggc tgaacgcggc atggagccaa aaaatacgaa ttctcccgag tggtgggcgt
     1921 ggcgctggtc cgcacatttc ggactttgcg aagatgagaa tgaaattgcc agcggcaata
     1981 gtgcatatgg cgctctgaac ttggctttcc atataggatt caaacatgtc gccctggtgg
     2041 gcgttgacgc tacacaagaa ccacgcgttc actccggcgg cacgccaaaa aatctaagtc
     2101 acctgccttt gttattccag tctgcgcgtg aacagattga cgttgtttca tgcgggaaaa
     2161 tgggaggtat tccgcagatg actcttaaag aatggctgaa gaatacatga tggcaccaac
     2221 aatttatcac cgtatcgacg gtaccaaata caggaatgtc tgggttgttg gtgatctgca
     2281 tggttgctac accagactga tgtccgaact ccatcgtgtg gattttgacc cggcgcagga
     2341 tttactgata tcggtcggcg accttatcga tcgcggtact gaaaatgtcg aatgtctgga
     2401 actattgcag atgccctggt tcagggcagt gatggggaac catgagcggc tgatgattga
     2461 tgcgttaagt ccagatggca acgtgaataa ctggctaatg aatggcggac aatggttctt
     2521 catgctggac actgatcagg aaatattagc cagggcgctg gtggagctgg taaagcgtct
     2581 gccctatatc attgagttga acaccgggca agaaactatc gttatagccc atgccgacta
     2641 tccggataat gaatatcaat tcggtaagga ggtgccgctt ttcaacgttg tctgggcgcg
     2701 cgagcgtatc agtgattcga tggatgatat tggtggcgaa atttcgggcg cagatcgttt
     2761 tatctttggt cacactccgg tgaaaagccc gaagacattc tggaatcagc attatatcga
     2821 cactggtgcc gtattttgcg gaaacctgac attgatgaaa gtgaaaggtg atggtgcagc
     2881 atgaagattg ctttagttct tcgctctggt ggtgactata acgcttccga tgtgcagtgg
     2941 ctggttaatc aactgccaaa aggctatgaa attatttgcc tgacagacct gaagcgttta
     3001 catgtacctg gcgtcaaagt tgtcccattg atcaaccagt ggcaaaagtg ccgtggctgg
     3061 tgggcgaaaa tcgagttgtt ccgaccggat ataaccgatg atctgttcta tctggatttg
     3121 gacacggtta ttgccggtga tatacgccca atccttgagg atccaccaac cagcttcacc
     3181 atgcttaggg atttttacca tccacaatat cgtggcagcg gtgccctgtg gataccaaat
     3241 agtgtaaaag cgcatatctg gagttcattc tggcaagatc cggaaggttg gatttctcgt
     3301 tgtgtcacta ctgagtgctg gggtgatcag gggttcttac ggaaggttat gggcgatgat
     3361 acaccagcat ttcaggatct gtatccagga tggtttgtaa gttacaaggc cgatgttgtg
     3421 gaaccaggtt caaaatatgc gagcgcgcgt tactccaggg ggaatggggc attaccaaag
     3481 gactgccgaa taattttttt ccacggcaaa ccgcgacctc gcgaagtgtc agaggattgg
     3541 attcccctca ttagctcgtt ttttgagcag gaatcagaat aatattgctc taataattcc
     3601 atatttttga aacgtgatgt acactcatca cgttttttat tagagcaatc tataaggtgc
     3661 actatgtggc cattccgacg gaaatatcac tactggctga tcgcctttgt tacgccgacc
     3721 ggcggtatca ggcatgtcat caccaggtat cgcaacaaga gactcacctt agccagaatt
     3781 ttacaggctg ccataggtga gggactggat acaaattgcg tagtccttcc tccttcatac
     3841 ttaggaaaaa tgaccgaagc acaagctaat acggaacttt gaaatgagca cttcagcaca
     3901 aaaccaatca atcgaaaatg tatctatccc tgatgtcctg aatgccggta tcccggccat
     3961 tatccagaac atccgggccg cgcaacgccg cgttagttgt gatgacctca cagcacgttt
     4021 ttttgataat gcggttcagt cagcggagat gcttcacgca cagcttattg atgtttataa
     4081 cgcagaagct gatagccata actccctggt agatgcagct gaaaatatgc agttggatct
     4141 cggtctgaag ggtaaagaaa ttgaagagct tcagctggaa attgaacatt tgaaacgcca
     4201 gcaacaggac gcgatcgaag atgcgacgca tgacgccaac cagcgtgctg ataatgccga
     4261 acgtataagc attgagctgg aaacaaaact caatgaaatg accgcgatgg ttgaactgcg
     4321 gaactcacag atttcaacgc taaaatctca atataaagag atcatgaaac ttgatccttt
     4381 taaccttgag aaacgttata acaaagctaa aagcgagcga caggaactgc gtaagcaggt
     4441 cgccgacctt aaccaacagc tcaaaaaaac tattaaagat gcaagcgaag cgcgcgtggc
     4501 atttgctaat aaaaaagcag aggttaccgc gctggttaat gagaatgcca aatttgcgac
     4561 gctcaagaag gaaatgtatg gcattactga gcgccgtttc cctgcaagca aacttcatcc
     4621 gacgttaggg caaatctcct tcttcccgcg cctcctggct tatgggatct catcgcctaa
     4681 agagttcaat aacgagcgtc cttatatcgt ttctaagctg gactttgctt atcagttctg
     4741 ctgcgacatg ggctatgcca ttgatatccg aatcaacgaa tggttgatgc caaacttcca
     4801 gccgttggca attttccgcg agttccagcc ggaaggttgg gtagagttct tccatgaatt
     4861 gatctgtaaa gagatggaaa gccgccgccc ggaactggtc cgtcgagttg agtgggcgca
     4921 agaggttatg ttagcagatg cagagctgcc gttcgaaccg gaattcattg atgatctggc
     4981 aactaaaggg ctgcataccc tgtttgatgt ggttacccgc cgtcatgagc agttggttgt
     5041 cgaattgggt ttagaggaaa ctgcggcaag aagacttctc gatgtttgct atgcacgtag
     5101 cgatgcatgg gaaaaagaga acggcggcac tatttacgtt cgctgatagt tacagtgtca
     5161 cctttaatgc tggtggagtg cgcccaccag catttttttc gtccaatgag gagggtattt
     5221 gagtattttc aataaacacg cacaccagga acgtccgtac atcgtcatag tagatattga
     5281 tgggacgata tcagaggcaa cggaagacag gctgcattta cttccaccac ctggcaaagg
     5341 tgcattaaca gagcactgga acgagtttaa ccttgcctgt gacactgatg ctcccatcac
     5401 tccagttatt gatatggtgc gccagttgtc cggcatttac accctctggt ttgtaaccgg
     5461 gcgctgtgag atagccaggg ataaaacacg agcctggttg cgtaagcacg taacaaatgg
     5521 ggctgagcct ttgctatcta tgcgtcctgc caccgatgac agaaatgacg gtccagcaaa
     5581 gattgatctc ctgaagaaaa ttggtctaag taaaattgcg ttcgcgctgg aagataagat
     5641 tgaagtggcg cgtgttttca ggagtcacgg cgtacttacg ttaatggtca gggagtatga
     5701 aaatgcgctt cttcatcaac aatgattgct ctaataaatc ttgattttta aaacagagaa
     5761 agtaaaaata aaaacatgcc gcaaggcgcg gcatgtatcc aatcaatcac aggagctgaa
     5821 aatatgaaca cggcattcaa aatcattatg gccgcgatct atttctggct gttctctatc
     5881 acttttggcg gcatcgtcgc gcatgggtaa gggggatgca tgaaaggcga agtgaaagag
     5941 cgcggcatga ttttcaacga tgagatggtc cgggcaattc ttggcgggaa taaaacacag
     6001 actcgcagga ttgttgaaga aaaattctat ggacgtgcag tggccgcaga gttgcttgct
     6061 aagcattgtc catatggtca accgggcgat cgtatttggg ttcgcgaaac ctaccgggta
     6121 catggcaaag cgacggacgt cgcaacgctg gtttatcgcg caagcgtgcg taactcctgg
     6181 acagaacaaa cgcaccgggt tccggtcgag gtttgtaata aaccagtatc agaaaagtgg
     6241 acgccatcaa ttcacatgcc gcgctgggca tcgcgcattc ttctggaaat taccaacgtg
     6301 cgcgttgagc ggttgaacga tatcagcgaa tgcgatgcaa gggctgaggg cgcaccaaca
     6361 gaatcgaccc tcattggcga taagcattac ccaggtttcc gcagcctatg gaaatcaatt
     6421 tatggcgaag atagttggaa cgctaatcct tgggtgtggg taatagagtt tgagcgtatt
     6481 cagggggcaa ccagtgagtg agtcaaaatg ccaaattaat ggcaacaaga tagaaccgtg
     6541 cgcggcgttg gcacaatccc tggagcatga cgctgaatac acgacgcgaa aaggtctgct
     6601 gaaatacaaa atctataacc atgaattaat tcattcacaa gacctgatca tgctgctgtc
     6661 tggtgaattt tctaaatcgc cgattcgagt ttcattttgc ccgttctgtg gtgaaagtct
     6721 gaaaacgtgg gaagcggagg caaccagtga gcaaaattaa ctatcaggca ctacgtgaga
     6781 tagcaaaaca ggcaacacag ggcgaatggg gcgcgtttat ttcgacgggt acaggtacgt
     6841 atgctgtgca tacgcccggt gataaacgat gtgaagatgt tatcaaatgg accggctttg
     6901 atggactgaa aaacgcagag aacaacgctc gttatattgc cgcattcaac ccaaaggttg
     6961 cactggagct acttggtgaa attaagcgcc tggaggacac aaatattgat gctatgtgcc
     7021 gaatagcacc gcttgagact aagctcgcgg ctctggtggc agagaacgcc gggctgaaac
     7081 acgcaatggc tgtaactctt gatcatgtgt cggtcacgga tgcagggcag gctggtgttg
     7141 ctgcaatgat tatcaacgat gccctacacc acagcgaaac tccagctacc gatgcttttc
     7201 tggctgaaat tcgtgcggaa gcacgcaacg aggggattaa ctataccgcc agccgtcttg
     7261 ctgctgcgtt caatcacgga tttatcaata agtctttgcg tgaagttttc gacgttacac
     7321 gca
//
LOCUS       DAZUFB010000170         7283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZUFB010000170 DAZUFB010000000
VERSION     DAZUFB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.170"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(334..1470)
                     /locus_tag="ACHY3M_004646"
     CDS             complement(334..1470)
                     /locus_tag="ACHY3M_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4319411.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYITQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1473..1835)
                     /locus_tag="ACHY3M_004647"
     CDS             complement(1473..1835)
                     /locus_tag="ACHY3M_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS4319412.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHY3M_004648"
     CDS             2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHY3M_004648"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFS4319413.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4515..5663
                     /gene="mtlD"
                     /locus_tag="ACHY3M_004649"
     CDS             4515..5663
                     /gene="mtlD"
                     /locus_tag="ACHY3M_004649"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4319414.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5663..6250
                     /gene="mtlR"
                     /locus_tag="ACHY3M_004650"
     CDS             5663..6250
                     /gene="mtlR"
                     /locus_tag="ACHY3M_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFS4319415.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6262..6471)
                     /gene="yibT"
                     /locus_tag="ACHY3M_004651"
     CDS             complement(6262..6471)
                     /gene="yibT"
                     /locus_tag="ACHY3M_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFS4319416.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6756..7118
                     /locus_tag="ACHY3M_004652"
     CDS             6756..7118
                     /locus_tag="ACHY3M_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFS4319417.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1851 a         1833 c         1844 g         1755 t
ORIGIN      
        1 ctacattgtg atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata
       61 aagccatcat cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca
      121 ggaggaacaa ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag
      181 aacaagacgg ataaaaaatc caatgagagc acactcatcc agaacttatt aacataatga
      241 atattaaaga aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca
      301 aggagcaaag ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat
      361 ccagctggtc attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga
      421 gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc
      481 cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac
      541 cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactggtca gtttgccgtg
      601 gaacacctgc ccaggtagcg cgttaaacac cacttctgca tcatcaccag gtttcagacg
      661 taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaaaac
      721 catcaccgga cgcaacggca aggcagctgc gtatgtacct gggcggatca gtacctgagt
      781 aatgtagcca ttgctcggcg cgcgaatgac agtctgctca agattatatt ttgcttcagt
      841 aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg
      901 gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa
      961 attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc
     1021 tttcagataa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt
     1081 gttggcctgc gcttctgtga gctgcgcgcg cagagtcttt atattatgcg tcgccgtcat
     1141 caggtcagcc tgaagcctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag
     1201 cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt
     1261 cacctgtggc gtgatgggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg
     1321 gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgccaccca gcgccgccgt
     1381 cgccagcgtc cactgattca ccggaatacg aaagatttta aagaccgccc acgccagcgc
     1441 cacgtaagtt aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca
     1501 gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg
     1561 atttccgggt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc
     1621 tccggttgat agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac
     1681 aggctcaccc agcccgccgt atgaatagcg tcggcatggg gatggttgcg ctttttggca
     1741 atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag
     1801 atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca
     1861 gtgttgtttt tgggctggca gccagaaggg agtcaggctg atattttgac aataatccgg
     1921 gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt
     1981 acataacgat tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta
     2041 agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga
     2101 cactactcac atttaaatgc catttttagc gaaaatcgcc gccttgttgc ttttttacac
     2161 aagcgttttg tgatgaacat cacgtcaatt acctctctac cccctatatt tatgtgattg
     2221 atatcacaca aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta
     2281 tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg
     2341 cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca
     2401 aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg
     2461 gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa
     2521 gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa
     2581 gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt
     2641 cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg
     2701 cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt
     2761 gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat
     2821 tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt
     2881 ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa
     2941 caatgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg
     3001 taaatcaatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc
     3061 gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca
     3121 cttcctgggt ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat
     3181 cctcgcagtc atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg
     3241 tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg
     3301 tgcttatttc gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc
     3361 tgctattttg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg
     3421 tcgtatgcag gacatgaaag ctgagtctaa aggcacatct ccgctgtctg cgggcgatgt
     3481 gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc
     3541 aagtgcgatg ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcacagat
     3601 ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctcg tcatcactca
     3661 ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcgcagcata tttcgctgac
     3721 caacttcctc gacagcggcc tgtacaccag cctaaccgaa cgtctcgttg ctgcccagcg
     3781 ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag
     3841 tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa
     3901 agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccgga
     3961 atacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat
     4021 cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt
     4081 ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt
     4141 gattggtatt gcagcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc
     4201 actggatgat gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct
     4261 ggaactgctg gcaggtcgta agtaatccaa tcccaccctc tccacatgga gaaggtgggg
     4321 ttaattgcct gatgcgctac gcttatcagg cctacaggat gcatcacaat ttgttgaatt
     4381 tgcacgttct tgtaggccgg ataaggcgct tacgccgcat ccggcgctgc ccctctcctc
     4441 acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga
     4501 tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta
     4561 tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg
     4621 tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggttggt gaaaccgagc
     4681 aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc
     4741 tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg gaacgtattg
     4801 ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa tccccgctga
     4861 acatcatcgc ctgtgaaaac atggtacgcg gtaccacgca gctgaaaggc catgtgatga
     4921 acgccctgcc ggaagacgcc aaagcgtggg tagaagaaca cgttggcttt gtcgattccg
     4981 ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg gaagtgacgg
     5041 tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca ctgccgaaca
     5101 tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc
     5161 tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc
     5221 gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg
     5281 gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg tacatccaga
     5341 aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc
     5401 agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc ggtacgctgg
     5461 aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg cacttccgca
     5521 gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg
     5581 cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa
     5641 ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa
     5701 cagattgtca gatatgcagg caacaatgga acaaacccag gcctttgaaa accgtgtgct
     5761 tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctttt
     5821 gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa
     5881 gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt
     5941 aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggagctgct
     6001 gatggcattg cgtgaagaac taaatcacga cggcaacgag tacgccttta ccgacgacga
     6061 gatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc cgccacagtt
     6121 tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg
     6181 atcaacaatg gtcctttcac tgactgagct gatttccaga atcagcttaa aaaaagcctt
     6241 tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat aaagccgacg
     6301 ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc cggacggaat
     6361 cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg atgccatgaa
     6421 atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca tgatgatatc
     6481 tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa
     6541 gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt ttattatctt
     6601 ttttctatag atttcttaat taatcaacga attgcgtagt ttttgagcat gttaatgatt
     6661 acgttgcaga actattacaa tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta
     6721 ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa
     6781 tcaaacgtct cagcgatcgc ctggacgcca tccgccacca gcaggccgat ctgtcgctgg
     6841 ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg gaagcagaaa
     6901 ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcacag aagctgatga
     6961 agatgccgtt tcagcgcgca attaccaaaa aagagcaggc tgatatgggc aagctgaaga
     7021 aaagtgttcg cggactggtg gttgtgcacc caatgaccgc gctgggccgt gaaatgggcc
     7081 tgcaggagat gactggattc tctaagactg cgttttaagc gcctcagacg actggttcga
     7141 tcatatcaac ccgcatctgg cttgccttag ctggtcaatg cggggactct cctgtctact
     7201 cgcctaatta ccaaacaatt cactcccttt acgtcgattt accgctggtt gatattctga
     7261 aaatgcattt ttaccgaaat agc
//
LOCUS       DAZUFB010000171         7276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZUFB010000171 DAZUFB010000000
VERSION     DAZUFB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.171"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(127..762)
                     /gene="msrQ"
                     /locus_tag="ACHY3M_004653"
     CDS             complement(127..762)
                     /gene="msrQ"
                     /locus_tag="ACHY3M_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFS4319418.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYTLLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(763..1767)
                     /gene="msrP"
                     /locus_tag="ACHY3M_004654"
     CDS             complement(763..1767)
                     /gene="msrP"
                     /locus_tag="ACHY3M_004654"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFS4319419.1"
                     /translation="MKKKQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1876..2289)
                     /gene="hiuH"
                     /locus_tag="ACHY3M_004655"
     CDS             complement(1876..2289)
                     /gene="hiuH"
                     /locus_tag="ACHY3M_004655"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFS4319420.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDNDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2422..3093
                     /gene="hprR"
                     /locus_tag="ACHY3M_004656"
     CDS             2422..3093
                     /gene="hprR"
                     /locus_tag="ACHY3M_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFS4319421.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3093..4451
                     /gene="hprS"
                     /locus_tag="ACHY3M_004657"
     CDS             3093..4451
                     /gene="hprS"
                     /locus_tag="ACHY3M_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFS4319422.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELEPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAVRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(4559..5410)
                     /gene="hchA"
                     /locus_tag="ACHY3M_004658"
     CDS             complement(4559..5410)
                     /gene="hchA"
                     /locus_tag="ACHY3M_004658"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFS4319423.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            5557..5967
                     /locus_tag="ACHY3M_004659"
     CDS             5557..5967
                     /locus_tag="ACHY3M_004659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319424.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSEMRGGGERFSDVEYCGA
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(6001..7164)
                     /gene="ompC"
                     /locus_tag="ACHY3M_004660"
     CDS             complement(6001..7164)
                     /gene="ompC"
                     /locus_tag="ACHY3M_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS4319425.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         1999 a         1542 c         1703 g         2032 t
ORIGIN      
        1 tgaattatat tgataatttg aggttgatga caaacgaaac ataacctgat gtgatctgcg
       61 taggccggat agggcgctcg cgccgcatcc ggcagggata acgcgtactt tgtcggcaat
      121 atgaagttat acagataatt tgttgtgaac ctgcttgcgt aaccggttaa aaagggaacg
      181 caacttctta taccgtaagg ctaaaagcag tacagccagc ccagcgtaga tgagcggttg
      241 cggtgaaata atcttcactg accacagata atgtattggg gccaggatcg cgacaagata
      301 gacgaaatta tgcaactgtt gccagtgctt gccgagtttt cgctgcatcg cctgggttga
      361 agtaaacgct aaagcaagca aaataaccca gctgataatt cctaacgtta aataaggccg
      421 ggtaattaac tccttgccta ataacgccag attattcacg cccagctcca gcaatgtgta
      481 actggttaaa tgcagtgtcg cccaggcaaa gcaccataag cctaacaggc ggcgagtgcg
      541 tatcaataac ggctgttttg cgtagcgtgc cagaggtgtg attaacaagg tcgccagcaa
      601 aaatttcagt gcagtgcgac cggtaaaatg ctgaatatct ttcaccggat cggcacccag
      661 tccaccgtga ttgatcgccc agaccagcca gagaaacggc aacaatccgg caagatgcag
      721 gctaactttc agccatgtca cctgttttgc tgtcagacgc attcagaaat tctcccgcaa
      781 atccaggcca cgatacagcg atgccacctg gtcggcgtaa ccattaaaca gtagcgttgg
      841 ctggcgctga acatcgagga tgccgcctga accaataaat cgttcggtag cctgtgacca
      901 gcgcgggtga tcaacatgcg gattaacgtt ggcgtaaaaa ccgtattcgt caggcgctgc
      961 cagattccag gtggttggcg gacgctcgcg ggtcagctta atactgacta tcgatttaat
     1021 ccctttaaag ccatatttcc acggcacaat cagtcgtacc ggcgcgccat tttgtggcgg
     1081 taacgccttg ccatagacac ccacggtcat cagtgtgagc ggatgcattg cttcgtcgag
     1141 acgcaatcct tcgacataag gatatttcag cccgccgccg ataaagcggt cctgctggcc
     1201 tggcatttgt tccggtgcat aaattgtttc gaaagcgaca tacttcgcat tgctggtggg
     1261 ttcggcaagc gccagcaatt tgtgcagcgg aaaaccaatc cacggcacca ccatcgacca
     1321 tgcctccacg cagcgcatac gataaatacg ctcttccagc gggaagcgac gggttaaatc
     1381 atcgtgatcg agggtcaatg gttttgccac ttcgccgctg attttcagtg tccatggatc
     1441 ggttttcagg ctaccagcat tagcggcggg atcggcttta tccagcccga attcatagaa
     1501 gttgttataa ccggagactt tatctactgg cgtcagtggc aggttatttt gccaggcggc
     1561 aggcttgctg aactccagcg gttttccggc gggggcgggc gggcgatcgt tccctttaaa
     1621 ccagctaagc agatcggcat gcgcagcgtg aggcaaagaa agtgcagctg cgctgatgcc
     1681 cagtgctttt aacacctgtc gacgcttcat aaagaatacc gactcggccg taacatctga
     1741 ttcttttaaa aattgcttct ttttcataag ccatcacact tttttgtcgt tagccagcaa
     1801 gagccgttta ctgttaatct gtcagggagt gtcctcccta acagtagcag agatctgctt
     1861 ttacatattt atgcattaac tgccacgata ggttgaatac ccatattggc taagtaataa
     1921 aggcacatga taatgctcgt tcactttatt aatatgaaat tcaacgggga tctcagggaa
     1981 gaaactttca agattttgtt tcttgaaata gtccccggtt ttaaatacga cacggtaatc
     2041 gcccgtagtt gcagtttgct cgggccacag tgccttaatt cgtccatcat tatctgtttt
     2101 ggcggtatta agttgtaacc agccgttgtc cgctttcttt tcaagagtga ctgtcacgtc
     2161 ggcagcgggt tttccggttt gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc
     2221 ggcataaacc aaagaaggta atgaaaatgc tgccgttact acggagagta ctaaataacg
     2281 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaagcggcc
     2341 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggctgtttc tataacatat
     2401 gatttatggc atattatttt catgaagatt ttacttattg aagataatca aaggacccag
     2461 gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
     2521 agagatgggc tttatcttgc gctgaaggat gattatgcat tgatcattct ggatattatg
     2581 cttccgggta tggatggctg gcagatctta caaacgttaa gaacagcaaa gcaaacccct
     2641 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggct ggacagtgga
     2701 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctggcaag ggttcgggca
     2761 caattaaggc aacatcacgc tttgaattca acattagaaa tcagtggctt aagaatggac
     2821 tctgttagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     2881 cagttacttt ggctactggc ctccagagct ggcgaaatta tacccagaac ggttattgcg
     2941 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     3001 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     3061 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attatctata accgtccgtt
     3121 taaccttgct ttttatattg ctgctgtctg ttgctggcgc tggaattgta tggactctct
     3181 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     3241 cgcagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtgtacttta
     3301 accggatgat ggatgttagt caggatatct tgatcattca tggtgatggc atcaataaaa
     3361 ttgttaaccg gacgaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     3421 tcagcgcagc tggcatttac agaagcatta ttaatgatac tgagattgat gctttacgaa
     3481 ttaatattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcca
     3541 gacataacat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccattg
     3601 tactttgctc agtattaagt ccgctgttaa tcagaacggg attacgagag atcaaaaagt
     3661 tgagtggtgt aacggaagcg ctgaattata acgatagccg ggagcctgtt gaggttagcg
     3721 cattaccgag agaactagaa cctcttgggc aggcgttgaa taaaatgcat caagccttag
     3781 tcaaagattt tgaacgccta agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     3841 ccattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     3901 aatatcaaaa aacaattgcc ggtaacattg aagagctgga aaatatttcg cggttaacag
     3961 agaacatact gtttcttgcc cgggcagata aaaacaatgt tttggtgaaa ctggactcgc
     4021 tttctctcaa taaggaagtc gaaaatttgt tggattatct tgaatacctt tcagacgaga
     4081 aagagatttg ctttaaggtc gagtgcaatc agcaaatctt tgcggataaa attttactgc
     4141 aacgaatgtt atcgaatctt attgttaatg ccgttagata ttcaccagaa aaatcgcata
     4201 ttcatataac cagttttctt gataccaacg gctatcttaa tattgatgtc gccagtcctg
     4261 gaacgaaaat tcatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     4321 gtcattccgt aggtcaggga cttggtcttt ctttagtcaa agcgattgcc gaattacatg
     4381 ggggaagtgc tacgtatcac tatctcaata agcataatgt gttccggatt atgttaccgc
     4441 aaagaaatta acatagggct tcagtacgcc ataaatcaac catgaaaaaa cgcagccacg
     4501 tcgtgagtac taacgcggct gcgattgatt atgcgcttac attcaaacgt aacagggatt
     4561 aacccgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     4621 aaggactgtc gccggtgaga actttacggt ccttatgtac tcgcccggtg atgtcgtcat
     4681 taatgatgtt catgcccatt ttcttcagtt cttcgccgaa gtaccaggtg agatgacccg
     4741 gcatatagcc aatctctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatggaat
     4801 aaccattcag tgggttatcg ccgtggcgaa gcgccagaaa agccgccggg ccgtggcaaa
     4861 gggagataac aaaacggtca tttttgattg cccactgcaa agcagcagct acgtcctggc
     4921 tttctggtaa accaataagt gcgccatgac caccaggaac aaagattgct gcatattcgc
     4981 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattgcgg aacaacgatt
     5041 tatgttgctc aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa
     5101 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     5161 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     5221 cggttggcaa ataacgttcg tccgcggcga tcaccagaat tttatgctta ccgcgatacg
     5281 gttttggata gtccacgcca tcaagatcag agacgggact ggtatattgg ctaagcgaat
     5341 attctgaagg gaagaacgca ttatcttcag caatatcgac ctgcggattt ttacttgttt
     5401 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     5461 gagctggtcg ctatttttaa ccaggctgga aaattgagtc aatatatcga aagcggtagt
     5521 cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     5581 ttttccttat tgagcaacgt cgtcgcaagt ggcctcaatg attttagacc attatcagag
     5641 atgcggggcg gtggggagag atttagtgat gtggaatatt gtggtgctga tgaatattta
     5701 tatcgcggat tggtagagaa aagattattt catgcactat ttttgcaaat atttctatat
     5761 gggaagcgtg tttgttacgg attaattcat aaagaaatgc atttgggtgt tggtttgaag
     5821 agtcgctggt tgatgttatt gtgccaggag gggcagggta aggtgctgta tcgatatgca
     5881 ttggcaattg cgtgtgccgc agatggagat gaaatgccaa taaaaaagcc tgaattcgat
     5941 gagggacgaa ctcaggctag tgtttagttt tacagtaaca cgccgcagca cgttggctga
     6001 ttagaactgg tagactaagc ccacaccaac aacgtcatct gttgcgatgc cattgttgtc
     6061 atagaaatcg tcatctttat ccaacaagtt tattttatag tcaacatatg ttgagaagtt
     6121 cttattgaaa tagtaggtcg ctccaatttc aacatagttg accagatcct gatcaacata
     6181 ctcttgatga ccattgttgt tatagtcgtt accgccgagg tctttgcctt ttgaataaac
     6241 atatgcaaga gatgggcgca gaccaaaatc gaactgatac tgtgctacag cttcaaagtt
     6301 ctgggtttta tttgcgatac ccacattacc atatggtgtc atattgcggg tttcagcata
     6361 catcattgca agataaacat tgttggcgtc gtattttgca ccaaatgtcc atgcttcagc
     6421 tgtatcacca ccagcaaatt tgctgtattc attgtagtga tacccgccaa gagctacttg
     6481 agaattggta cggtcagagt ttgagtaagc ggcacctaag ctcaaaccga agtcaaagtc
     6541 atatgaagtg gaaagaccaa aaccatcacc gttctggaag cgaacgtctt cgtgaccatt
     6601 tttagtacct tcttgatctt ctcctgcatt gctgccttcg ttgttacctt gatactgcaa
     6661 tgcaaagttc agaccttcca ccagaccgaa gaaatcacta ttgcggtaag tagctacccc
     6721 atttgcacgg cctgccatga agttatcagt ccaggtatag gagtcgccgc cgaatacagg
     6781 aagcatatct gtccaggctt caacgtcgta aatcacacca tagttacgac catagtcaaa
     6841 ggagcccatc tcgccgaact tcagacctgc aaaagcaagt ttgacataag tattatcaga
     6901 accttcagtg tcgctagcct ttgttttggt cagtgcttga ccataaccag ttaactgatc
     6961 tgtaatttgg gtctcacctt tgaagcctaa ctcgatgata gtttcatcgc cgtcatcgcc
     7021 agggttatcg gaaaaagtat gacgagcatc aacttttccg tacagatcca gtttattgcc
     7081 atttttatta taaatttcag ccgcatttgc tgcgccagca actaataacg ccgggaccag
     7141 cattgccaga acttttcttt tcattatgta ttcccttgtg attataatct tcatgaatat
     7201 atcaataagt gccgttatcc aaaaaaaagc acatttggat actattctat gaagtttatt
     7261 ttattttaag gattac
//
LOCUS       DAZUFB010000172         7208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZUFB010000172 DAZUFB010000000
VERSION     DAZUFB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.172"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            724..1890
                     /gene="dacD"
                     /locus_tag="ACHY3M_004661"
     CDS             724..1890
                     /gene="dacD"
                     /locus_tag="ACHY3M_004661"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFS4319426.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAIDGQRRLIAVVMGADSAKGREEEAKKLLR
                     WGQQNFTTVQILHRRKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLN
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            2005..2478
                     /gene="sbmC"
                     /locus_tag="ACHY3M_004662"
     CDS             2005..2478
                     /gene="sbmC"
                     /locus_tag="ACHY3M_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFS4319427.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            2676..3735
                     /gene="yeeA"
                     /locus_tag="ACHY3M_004663"
                     /pseudo
     CDS             2676..3735
                     /gene="yeeA"
                     /locus_tag="ACHY3M_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            3907..4236
                     /gene="tmaR"
                     /locus_tag="ACHY3M_004664"
     CDS             3907..4236
                     /gene="tmaR"
                     /locus_tag="ACHY3M_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFS4319428.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4337..4519)
                     /locus_tag="ACHY3M_004665"
     CDS             complement(4337..4519)
                     /locus_tag="ACHY3M_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFS4319429.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            4573..4719
                     /locus_tag="ACHY3M_004666"
                     /pseudo
     CDS             4573..4719
                     /locus_tag="ACHY3M_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5008..5088)
                     /locus_tag="ACHY3M_004667"
                     /pseudo
     CDS             complement(5008..5088)
                     /locus_tag="ACHY3M_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5134..5328)
                     /gene="yeeW"
                     /locus_tag="ACHY3M_004668"
     CDS             complement(5134..5328)
                     /gene="yeeW"
                     /locus_tag="ACHY3M_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4319430.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(5325..5698)
                     /gene="cbtA"
                     /locus_tag="ACHY3M_004669"
                     /pseudo
     CDS             complement(5325..5698)
                     /gene="cbtA"
                     /locus_tag="ACHY3M_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(5787..6155)
                     /gene="cbeA"
                     /locus_tag="ACHY3M_004670"
     CDS             complement(5787..6155)
                     /gene="cbeA"
                     /locus_tag="ACHY3M_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFS4319431.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADHAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(6229..6450)
                     /gene="yeeT"
                     /locus_tag="ACHY3M_004671"
     CDS             complement(6229..6450)
                     /gene="yeeT"
                     /locus_tag="ACHY3M_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4319432.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6519..6995)
                     /gene="yeeS"
                     /locus_tag="ACHY3M_004672"
     CDS             complement(6519..6995)
                     /gene="yeeS"
                     /locus_tag="ACHY3M_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS4319433.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7011..>7208)
                     /locus_tag="ACHY3M_004673"
     CDS             complement(7011..>7208)
                     /locus_tag="ACHY3M_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4319434.1"
                     /translation="WVLFNAMNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHY
                     YRLRDYALQHPECSAIMRIID"
BASE COUNT         1815 a         1671 c         1836 g         1886 t
ORIGIN      
        1 gtattgaatt tagcctagtg gctaccatcg ctttctcctt ataaaaatat aaagtatata
       61 tttgaatgca ttctagtttt atttcaatcc agcatcaagc cagtctgccc accactgcat
      121 catttctctg cgcttaatgt agcggatgcc tcactatcac atagtgatgg ttcattactg
      181 gtttgcataa tagatagaga actgaggata aaagatatca caaaacacca aaatctcatc
      241 tttaggattt gcagaaaggg agacgtaaac aaatacctgc gcatgaaaac ggaactagtc
      301 cagatgtgat gtttgtggtg atcacataca tcattaacga tgctcgttat ggtgagtttg
      361 atgactaccc gatgaagtga aaatagtgtt gtgtaccaaa ttgcgtacca aactaaaatc
      421 acaaatcatg aaacccttgt tcatggcggt tctcaggggt attgcgcgta atcgtgaaac
      481 aaaaaggtag attgttgctt accgtcattc atcattaggt taaatccgtt atttctgctg
      541 tctgccagag tatcaaatat caccgtgcta atcagcttta gcgcgacaat ttggcagcga
      601 gtgacaacag atcatgtcag ataaaaatga gatagtagtc acattttctt gcactttatt
      661 ccagccagtt cataagtatt tccgtaaaaa gaacagctat ttgaaacacc tgagggtttg
      721 ctgttgaaac gccgtcttat tattgctgct tctttgttcg tttttaattt atcgtctggt
      781 tttgcggcgg aaaacattcc tttttcacct cagcctccag agattcatgc cgggtcctgg
      841 gtattgatgg attacaccac cggtcagatt ctcaccgcgg gtaatgagca tcaacagcgt
      901 aatcccgcca gcctgacaaa gctgatgacg ggctatgtcg tggatcgcgc tatcgatagt
      961 catcgcatta cgccagacga tattgtcacc gtggggcgtg atgcgtgggc gaaagataat
     1021 ccggtgtttg tcggttcttc actgatgttt ttgaaagagg gcgatcgcgt atcggtacgt
     1081 gatttaagcc gaggtttaat tgtggattcc ggaaatgacg cgtgtgttgc tctggctgac
     1141 tatattgccg gtgggcaacg gcagtttgtt gaaatgatga acaactatgc cgagaagctg
     1201 catctcaagg atacgcattt tgaaacagtg catggtctgg atgcacctgg ccagcatagc
     1261 tcggcttatg atttagccgt gctttctcgc gctatcatcc acggcgagcc cgagttttat
     1321 catatgtaca gtgagaaaag cctcacctgg aacggtatca cccagcaaaa ccgtaacggg
     1381 ttattgtggg ataagaccat gaatgttgac ggcctgaaaa cgggccatac ttctggtgcc
     1441 ggatttaatc ttattgcttc ggctatagac gggcagcgtc gcctcattgc agtggtaatg
     1501 ggtgctgaca gcgcaaaagg tcgtgaggaa gaggcaaaaa aattactgcg ttgggggcaa
     1561 caaaacttta ctacggtgca aattttgcac cgtaggaaaa aggtcggtac ggaacgcatc
     1621 tggtatggtg ataaagaaaa tatcgccctg ggaacggaac aagagttctg gatggtgcta
     1681 ccgaaagccg aaattccaca tatcaaagcc aaatataccc ttaatggtaa agagctcacc
     1741 gcgccaatta gcgcccatca gcgggtaggg gaaattgaac tttacgaccg tgataaacag
     1801 gtggcgcact ggccgctggt taccctggaa tctgtcgggg aaggcagcat gttttctcgc
     1861 ctgagtgatt atttccacca taaggcctga cctttctttt gcagcagact ggagtgcgag
     1921 tctgctcgca taatcaacac tcattccttg tggttttaat attgcaacta tactgtatat
     1981 aaaaacagta ataatggagg cgtcatgaac tacgagatta agcaggaaga taaacgtacc
     2041 gttgcaggtt tccatctcgt tggcccgtgg gaacagacgg taaagaaagg ttttgagcag
     2101 ttgatgatgt gggtagatag caaaaatatt gtgccgaagg agtgggttgc tgtctattac
     2161 gacaatccgg atgaaacacc cgccgaaaaa ttacgctgcg acaccgtcgt gacggtaccg
     2221 aataacttta cgctccccga aaacagtgag ggcgtcattc tgacagaaat ttcaggtggt
     2281 caatatgcgg tggcggtggc tcgtgtagtc ggtgatgatt ttgctaaacc ctggtatcag
     2341 ttctttaata gcctcttgca ggacagtgct tatgaaatgt taccaaagcc ctgcttcgag
     2401 gtttacttga acaatggcgc ggaagatggg tactgggata tcgaaatgta tgttgcggtg
     2461 cagccaaaac atcactaatt catctcaggg cggtgtgttg acgcgaagac cactcttttt
     2521 ttgaaagcga aaagagtaag atgcgccttt caattttttc gctcctgccg ggaaattaca
     2581 ctgttcccgg tttgtccgtc ggataattca gaggcgcgcc ttctggccga cagatgagtt
     2641 atgagcgctt ttaatcttca ttacggagtt tctgcgtgcg tgccgataag tcattaagcc
     2701 cgtttgaaat ccgggtatac cgccattacc gcattgtgca tggtactcgg gtcgcgctgg
     2761 cattcctgct cacttttctc attatccgcc tgtttactat cccggaaagc acctggccgc
     2821 tggtcaccat ggtggtgatt atggggccaa tctcgttctg ggggaacgtt gtccctcgcg
     2881 cattcgaacg tattggcggt acggtgttgg ggtcgatttt aggtcttatc gctctgcaac
     2941 tggagttaat ctcgttaccg ctgatgttag tctggtgcgc ggcggctatg tttctttgcg
     3001 gttggctggc gctgggcaag aaaccgtatc aaggtttatt gattggggtg acgctggcaa
     3061 ttgttgttgg ttccccgaca ggtgaaatta atacggcgtt atggcgaagc ggcgatgtga
     3121 tcctcggctc tttactggca atgttgttta ccggtatctg gccacaacgg gcgttcatcc
     3181 actggcgcat tcaactggca aaaagtctga ccgagtataa tcggtatcta tcaatctgca
     3241 ttctcaccga acttactcga acgcccgcgt ctggaaagcc atctacaaaa actcctgacc
     3301 gatgccgtga aaatgcgtgg gctgattgcg cccgccagca aagaaacccg tattccaaaa
     3361 tcgatatatg aaggtatcca gaccattaat cgcaatctgg tttgtatgct ggagttgcaa
     3421 atcaatgcat actgggccac gcgccccagc catttcgtgt tattgaacgc gcaaaaactt
     3481 cgtgataccc agcatatgat gcagcaaata ctgctgagcc ttgttcatgc gctgtacgaa
     3541 ggtaatccgc agccggtttt tgccaatacg gaaaaattga acgatgctgt ggaagagctg
     3601 cgtcagttgc tgaataacca ccatgacctg aaggttgtgg aaacaccaat ctatggttat
     3661 gtgtggctga acatggaaac ggcacatcag cttgagttgc tatcgaatct gatttgccgg
     3721 gccttgcgca aataattcct gaacttcaga atcatcttgc tgctgcttcg attcagcaag
     3781 gataaagggt atgatagtga aaagggataa aagcattgtc atctgcggca gctatgagta
     3841 atgttggccc taacgaatag cggttgctta aacgaatccg actctcacat gatcaggggt
     3901 ataaaaatgg aaactaccaa gccttcattc caggacgtac tggaatttgt tcgtctgttc
     3961 cgtcgtaaga acaaactgca acgtgaaatt caggacgttg agaaaaagat ccgtgacaac
     4021 cagaagcgcg tcctgttgct ggacaacctg agcgattaca tcaagccagg gatgagcgtt
     4081 gaagcaatcc agggcatcat cgccagcatg aaaggtgact atgaagatcg cgttgacgat
     4141 tacatcatca aaaatgccga gctctccaaa gaacgccgcg atatctccaa aaagctgaaa
     4201 gctatgggcg aaatgaaaaa cggcgaagcg aagtaattcc cgttttattc aatgagggtt
     4261 gcccggcaac cctcattgct cattgattct tatctgtgta tcaccgtcat cattctcatc
     4321 tgagaaccaa tcgaaattaa caacagcctt cttctgtatg cagcaaggca aaaagttctg
     4381 taactccatt gttattaact gcactggtta ctaacacgtt gtgcgctcca gcttcccgta
     4441 accaactttt caccaaagat atttgttcca tgctggctaa atctgctttg gttactactc
     4501 caatgaccgg gtgattcatg gcccggtagg gcgtttttac ctctaattcc tgcttcagcg
     4561 ctgagattcc tgcttgttgc cggaaaagca ggcacctgcc gtctgaacgg tatcgatccg
     4621 gaagcgtatc tgcgccatat tctgagcata ctgccggaat ggccctccaa ccgtgttgac
     4681 gaactcctgc catggaacgt agttctcacc aataaataag cgtcaatacg gtgctccgtt
     4741 gacgcttacc ttaatccgtt ttctgagctt agatttttct gttcctaccc agaccgtgga
     4801 tttggaattc attttttgtc aggtcaatgc taatcgttaa aacggccata acgcacctcc
     4861 actggaaggt ttatcagtaa tatcctaccg ttgcttcatg gtcggggcgt ccattccatt
     4921 attatcggtt ttcctttctg tttataaatg aaaacgccag ctgtattcag gctggcgtca
     4981 ggggaaatga agcctgttga gtgagattca ccggttctgg tgcagaagct gcagatggcg
     5041 cagggtaagg acatcttctc ttgcctgctt aatacaggac aacagtgctg tttctgtggt
     5101 taatggcact ataccctgca tcactgcatt gtcttacagc aactggtgag ggctggcata
     5161 agttgccggt gtttcggtag agtcacggtc atccaccacg cggagtgaat aacctttttc
     5221 agcggcctta ttaatcagtt cagtgatatt aacaccatca atgtcaacga caatgtgccc
     5281 catatccagc gcctgtacgt taacgctgtc ggcttccagt gtcatcattt cgcctccgga
     5341 tacttaccca gggtaatgtt atttaccgtt ctgtaattgt cgcgggtcat caggccggtc
     5401 gccctgcgag cccggaggat atcgatgctg tttattaact gagagcgggt acaggcgctg
     5461 aatcccggct ggtcggtagc accagcgcgt atttttccac gagaaagttc accgcatcac
     5521 acagtgaaat gcctgcctca atatgctgct caatcacacg ttcatcggca aaaggtgtgt
     5581 cattcagtgt gaggccatag tgctggtcca gcagtcggga cagcagtatc tgccagattt
     5641 caacaggaga cgggcgagaa ctggtcgcct gcccgggtaa tacaggtaat gttttcatac
     5701 tgaagatttt cctgatatgc agatataaaa atggaaaagt ggcgtggtga aaacaccaga
     5761 ccgtagcaga aggctattct ggagagttaa tttttcattt cgggcgtcgg ataaacagcc
     5821 agataaacgt aaccacaact gctgagggta tcggctttgc aggtcagccc ttttgcatac
     5881 agcgtgacgg tatgctgatg gcggggattc agttcaccgc tggtgagcat gagttccagt
     5941 tgtttcatca gcagcggaaa ggcctggtcc aggtggtacg catctgcatc gctgaacagg
     6001 cctctaatac cggcgtggtc ggcaaggtaa tgcaaccggt taccctcctg caccagacgt
     6061 gccccgaaac agggcgtcac ggtgcagggc agcccccacc aggggcggtc gtgattgtcg
     6121 tcgggaagtg ttgtcccggg gagtgtgtct gacacgataa aatccttaca gaaaatcggc
     6181 taagaatggt ccggtagtgg cgataattct gctcatcaga attcccactc agttcagggt
     6241 gacgctcatc agccggacat acggaccaaa actgtcctta cggcgttctg caaacacggc
     6301 cagcacaccg ggaatatcct gcacttcacg accggtatac gcttcagcac tgccgtgcca
     6361 gcggtatttg ccggtacaga acggaaaaag acgggatgcc ggatgctgac ggtgaatacg
     6421 catggcttcg ccacgggtga tgattttcat agtgggatac ctctgaagac agaagataaa
     6481 agtgaaaaca ggtgtgatgt ggttgtaacg gtgacgggtt aaagcagacc gtgttccgca
     6541 aaggagaaaa cctggttgcc accaactatc agatggtccg gtacccggat atccaccagg
     6601 cccagtgcct gtaccagacg ttccgtgata aggcggtctg ccttgctggg tgtgacttca
     6661 ccggacgggt gattgtgtgc cagcaccaca gcagcggcat tgtgatacag ggcgcgttta
     6721 atcacttccc ggggatggac ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg
     6781 gcaatcagct gattctggtt gttcagatac agcacccgga attcttcacg ctccagtccc
     6841 gccatgttca gaatcagcca ttcccgtgct gcacgggtgg aggtgaaggc cacgccgggt
     6901 tcatgaagat ggcggtccag ggttttcagg gcccgttgaa tgaggctgcg ctcgccgggc
     6961 gtcatctctc cgggcagaaa ggaaagttgt tgcattgtgc ttctctccat tcagtcgatg
     7021 atgcgcataa tggcgctgca ttccggatgc tgcagggcgt aatcccgcaa ccggtaataa
     7081 tggaccgtca tggcataatt ctccgtacga caggcatgat gactgtacgt catcagacag
     7141 gcggcaatac cggcggcttc cgggctcatt tctgcgcggt taccgttcat ggcattgaac
     7201 agtaccca
//
LOCUS       DAZUFB010000173         7160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZUFB010000173 DAZUFB010000000
VERSION     DAZUFB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.173"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..282)
                     /locus_tag="ACHY3M_004674"
     CDS             complement(<1..282)
                     /locus_tag="ACHY3M_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFS4319435.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLE"
     gene            complement(263..490)
                     /locus_tag="ACHY3M_004675"
     CDS             complement(263..490)
                     /locus_tag="ACHY3M_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS4319436.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRICADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            complement(501..719)
                     /locus_tag="ACHY3M_004676"
     CDS             complement(501..719)
                     /locus_tag="ACHY3M_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319437.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(703..1131)
                     /locus_tag="ACHY3M_004677"
     CDS             complement(703..1131)
                     /locus_tag="ACHY3M_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319438.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHTEEQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            complement(1166..1666)
                     /locus_tag="ACHY3M_004678"
     CDS             complement(1166..1666)
                     /locus_tag="ACHY3M_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4319439.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(1738..2163)
                     /locus_tag="ACHY3M_004679"
     CDS             complement(1738..2163)
                     /locus_tag="ACHY3M_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS4319440.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(2233..2742)
                     /locus_tag="ACHY3M_004680"
     CDS             complement(2233..2742)
                     /locus_tag="ACHY3M_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFS4319441.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNQALKPGTVTAKIRAIWRVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(2739..3035)
                     /locus_tag="ACHY3M_004681"
     CDS             complement(2739..3035)
                     /locus_tag="ACHY3M_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319442.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            complement(3025..3222)
                     /locus_tag="ACHY3M_004682"
     CDS             complement(3025..3222)
                     /locus_tag="ACHY3M_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319443.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            complement(3215..3547)
                     /locus_tag="ACHY3M_004683"
     CDS             complement(3215..3547)
                     /locus_tag="ACHY3M_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319444.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(3563..3913)
                     /locus_tag="ACHY3M_004684"
     CDS             complement(3563..3913)
                     /locus_tag="ACHY3M_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319445.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(3928..4239)
                     /locus_tag="ACHY3M_004685"
     CDS             complement(3928..4239)
                     /locus_tag="ACHY3M_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319446.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            complement(4236..4787)
                     /locus_tag="ACHY3M_004686"
     CDS             complement(4236..4787)
                     /locus_tag="ACHY3M_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFS4319447.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            complement(4791..5306)
                     /locus_tag="ACHY3M_004687"
     CDS             complement(4791..5306)
                     /locus_tag="ACHY3M_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319448.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQTVKDEAKRLEAVLNVACRSVFE
                     FSISGFCFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(5306..5839)
                     /locus_tag="ACHY3M_004688"
     CDS             complement(5306..5839)
                     /locus_tag="ACHY3M_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319449.1"
                     /translation="MIDAKVLEGVKNWLRIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMSYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            complement(5843..6385)
                     /locus_tag="ACHY3M_004689"
     CDS             complement(5843..6385)
                     /locus_tag="ACHY3M_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319450.1"
                     /translation="MAYFYFKLDIVYTNKYLTEYQRDVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGGGPCFIARPDESCPEGPAIAAMVEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGTYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            complement(6483..7013)
                     /locus_tag="ACHY3M_004690"
     CDS             complement(6483..7013)
                     /locus_tag="ACHY3M_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS4319451.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRLMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            complement(7025..>7160)
                     /locus_tag="ACHY3M_004691"
     CDS             complement(7025..>7160)
                     /locus_tag="ACHY3M_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319452.1"
                     /translation="SILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ
                     "
BASE COUNT         1700 a         1993 c         1672 g         1795 t
ORIGIN      
        1 ctccagcagc agttcgttct gataggagac agagcggagc gtggcgttcg tggttttcag
       61 ttcaccccgc agggtcacga tttccacatt cagacggttg acctcggctt ctgtcggcag
      121 tgacgacagg cgggtttcca ccttctcaac gcggtcggag agtttttcaa aggcctcgcg
      181 ggggacaaac gttttgcgca tcagcgccat gaatatcccg ccagcggttg ccgtcgccga
      241 aaggatcggc acaacataat ctttaacgat gctgacccac atgacgggcc tccatgatgt
      301 tctggcagtt cacacaacgg atggcatccg gcacagcaac aagacgcgcg gcggggatct
      361 ctccgccaca gtccgcgcaa atacgtctac ccggagcatc cattgcccgg cgcttacgtg
      421 tcagccggtt acaaagggcg cgttctgatc cccgctccat cacagcctga gcacgatctg
      481 aatcatccat acctttttcc ttacttcgtc tttcctgccg gaacagcagg cgttccggcc
      541 atatcagtaa attcatgttg tcgccgcgcc tccagacgac gaatactggc cttgtccacg
      601 ttgcagttct tgatcaccgc cagcagttgc aggttgtaag tgaccgatgc cccaaacgtg
      661 aacggctccg gcgcaggagg gaccaggcag tcagccagcc attcagcggg gatcggcacc
      721 ggcttcacag gcacgtattt cacagacggc ccggcgcacc cgatcagcca catcatcagg
      781 aacaggggca gaggcagcag gcactgctgc cagcgaccgg cggacaacgc tctgttgctc
      841 ctcggtgtgc tgaatgttct gttgcttttc attcgtggcc gcctcactga tttgtgaaat
      901 gagttgcagg gtgtgctgct gatttgccag cacctgacgc gcctcatccc gctcacgtac
      961 aagtacctga ttattgtcct caaggcgctg cgtcttctgg tgcattcccc atatcgcagc
     1021 aaccagtaca gcgataatcc ccagcgccgc catgcacgcc ccggcaatca gggccggtaa
     1081 ggcgttattc accgcagcca caggacaccc cgtaacagca gtaccgcaca cagcagcgct
     1141 atcatgagcg gtcgccagaa gcggttcaga ctttcggcaa gtcgtagcga cataaccatt
     1201 catccgccga acggcgggcc tcaaggccgg gaagtttcac cccctttgaa taaatccagc
     1261 ggatatactg cttacaggac gcgggcatct gcccggcatt gatcaggcgc agaagcgtgg
     1321 agttgcggaa gttcgtttca ccagcccaga agatccacga agccagcgcc accgtctgac
     1381 cacgggtaag cggcacctga acacgccgat caatggccgc aaaggcccat ttcatgtcct
     1441 tttccagtag ttccaggcat tcttcatccg tcagggtcat acccggtttc acatccgggc
     1501 cggtatggcc gtaacagatg gtgggtgtac cggtgggatc gatatacgtg gagttttctt
     1561 tcccttccca gtaaccggtg taatgcgtgg caatcgtgaa cgtcccggca ccggccagaa
     1621 caagggcaat cagcttttta cgcagtggtg cggggagttt tggcattgtt acgctgtctc
     1681 atgatgttct ctgagtcagc gtaacggtga aggaaaggcg gcaggctttg cggaatatca
     1741 gaaaagtggc ggttgcgttc ttgccagatg cagacggcgc tgttcacgta tgacggtata
     1801 aatctgggtt tctgacatcc ggtaatgtcg gcgaagggat tcaattttct cgccccggtc
     1861 ccagcgcgaa aatatctcat tgtttcgcag ctcggcaaag agagattccc cgaccggcag
     1921 gtaataaccc cgtccgccca tataggcggc ctgagccgca gccaccttgc gtgccagttt
     1981 accggccagt accggctcaa tcccctgacg ctgaagttct gcgccggtga cttcaaccag
     2041 ttctgacagg gtccgtggcc agttcttttt gagaacatca tcagggatgt catcaaggcg
     2101 gtcgataagg gcgtgcagtt gctcactgtc accaccaaac atgctcatct gagtttctgc
     2161 catatcagcc tccggttctc gcgttcccgg taagtttaaa ataaaaatcc cgcgctgtgg
     2221 cgggatttgg ggttaacggg gaagtgattt ttcatactgc ctgcaaacgg cgtcataccc
     2281 catcagcgtg cgttcgggca ttcccatcgc ggccagcatt ttgcggcggt gccatcgctt
     2341 gagacgctcc agcacatctg aggccagggc ggggtgttgc tccagccact gccagtttgc
     2401 cacaccttcg ccaccgttct gcgcggccgt ctgcgatttc acccagcggt taagggcggt
     2461 ttccgcacca tcagagataa agccctgccg gtgcatcacc ctccagatgg cacgaatttt
     2521 ggcggtcacc gtacccggtt ttaaggcctg gttaaccggc ttctggcgca ctttaaaacc
     2581 gcgttttttg aaaacatcca gcacgcggga taactcatcc ggtgacatat cccggcagct
     2641 ggttttgccg gtggctgcca gcagcgcgga tgtataagtc tcatcatcca gggcaagctc
     2701 gcgctttgcc acatgaatgg cggatatcag ttttcctctc ataccgtatt taccttttta
     2761 cggacatgga aagccttttc tgcctcatcc caccgggcat ggtgtgttgg tggaataatc
     2821 attatgtgcc agccatctct ggttgttatt cggcagaaaa attcaccgct atataacaca
     2881 atggcctgct gaatgagcgc atcagccgcc cgttcaaccg gatgtctttt atcaaaaaag
     2941 cagatgattt ttcgacataa ccaccgggta ataacaaatg cgccgaaaaa ggatgtgata
     3001 tataagcacc agaacaacag agaactattg tccattatct ttctccttat tcactcttct
     3061 gcggcattcg gcacagtcat cagggctttc aaatgtatcc ggctcgcgat tatgagccag
     3121 atataaccac cgtccacata tactgatatt ttcgcctgtt ttaaaaaaat gatgcttcct
     3181 ggccattagt ggccgcgccc atccagcggg tagactattc atttgcttta tcctccatac
     3241 tggagagaat catatgtatt aatgtcgggc gtggagatat acgcatttca ttaagcgtct
     3301 gaagggcagc aacagctgcc tgtttatcgg tgcctttggc aatttcagca agagaatgca
     3361 caatccataa cgggcttaac gctacggctg aatgcagaat catgtcatcc ataataattt
     3421 ctggcatatc ccgtttgtca cattctttat ccataaaaga aacaattttt tctgcatctt
     3481 ccggtttcat gcctccggcg atgagaattt tacgatattc ataagacgat cttaattcaa
     3541 tactcatcat tcacctgcct gtttaatcag tttccagtgc tttgctacgg gcaacagcct
     3601 cacactcaaa aaataccgac tccggcacac gctcctttaa ttcacgaaca agaaaatcat
     3661 taacgagttc atgacgtctt atatttcttt ccttttcctc ctggcgcaat acagcaaggc
     3721 ggtattcaac agtccggcgt tgtttctgaa agacagccag tgcttttctg gcagagttac
     3781 gccatgtatc atcgccacag ggttcgtatt caagctgaaa tttaatctct tcaatggttt
     3841 cttttatttt tgccagcaag ctaagacagt cgctgattgt cttcagtttt tcattatcaa
     3901 tataacttct cacgcattca cctcccgtca ttccggtaaa gctgaaatat ggcaccagtg
     3961 cgtcacctgt ccgtcctcat cttcaaggca ggctccggtt tcttcatcaa taaaaaagcc
     4021 ttcatacccc atatggccga tgcgcagttc ctgttggtca ccatactgaa caatcagcac
     4081 gacagcctcc atatcaggcg ggaaagcatc attcagtgaa tgccagggat aaccgcgcaa
     4141 atccccgaaa taaacaccgg tcagcgttcc gccacagacc ggcattccga cggcacacac
     4201 aaggggttca tctgattcaa caaataatgt cgttatcatg gcattaattc ccgtaataca
     4261 acatcttcgg cagtccgacg acggtgataa ataaaactca ccgtgccctc ggataattca
     4321 aatttctcgc cgatttccct aaaggtaaga cgccgtggtg attcagcatc ccgtaattca
     4381 cgaataagac gaacatcatc gtcaggtatg cgggtgaagg gcagcaactc tccgtatttt
     4441 ttcatgctta cgccaatata tcgcgcccga ttgtggacag aatcccggtc tcgccccaga
     4501 tattctccta tttgcttacc ggtcattgtt ctggcatttt ttcggataaa tttatcttca
     4561 tcaggtttaa atctgaggcg tttgcatttg agcaactccg ggtaagtacc gcgtaataaa
     4621 acaatccggt tatgtacacc ccagaaactt ctttttatcc tgacagcaat atccttaacc
     4681 ggggtggatg gataaagcgc aatcagcagc gcatcttctt caggtgtcca tgcgcgaaca
     4741 tgagcgggtg cacggccttt tctccccatg ggttgtaata tcatcatgca tcaccccctg
     4801 actcgctttt cagcagcata aaactcatca gacagaattt cggtcatttt ctgttttgcg
     4861 ggttttacgc cgcactgaag gtaaatcaca tcgtcaacgc agaaccactc cacagggcca
     4921 aacaggattg ccgagaaatc aatgcccagc cagaacaaca gtgcatcggt tctggcatac
     4981 gtgacgggcg aatattcacg ccacagactg ttaagttcgt cagaggcaac gcgcagggct
     5041 ttgggaatac gtgatgtgcg cggtgtgcag ctccagccat tcgacgcagt tggttttcgc
     5101 cataaatcac gatgaaaagg atatttgtcc tccgtaaaac gcagtccctt aaaacaaaaa
     5161 ccactgatac tgaattcaaa taccgaccgg cacgcaacat tcagcacggc ctcaaggcgt
     5221 ttcgcctcat cttttaccgt ctggcagtcc tgctggtatt tttgccacgc agccagcgcg
     5281 gaagggtttg atgttttaaa gaacattacg ccacctccgc tgatatcccg gcaggcgcgg
     5341 ccgtccggtc aacaatcagg taacgcagaa cgcgcgaggt gatattccag ccgcttaaac
     5401 cgcagataat gacgcccagg cggatgtcga tatacgacat cacaaactgg ggcacgcctt
     5461 ctttcatggc ctgctcatca acttcagcca ccacataaac ggtttcacag gtcagcacgc
     5521 cctgagcaaa ttcccacgcc atcgacggaa tatcatgccc ttcaatccac ggcagggatt
     5581 ttctgaagct gcaccactga acatcatcag ccgcagactc ctggctgcat ttgcgacgaa
     5641 ccggctgcac cgggcgggga cgcggaatat cataaaaacc gttacattcc gtcagcacgc
     5701 ctgcatcaac cgcatcacgc agaaaataaa ccatggagga tggcggcata ttcatttttt
     5761 cagccagaat accgcaggtc agacgtccgt aaatacgcag ccagttttta accccttcaa
     5821 gcacttttgc atcaatcatg aattattcct ccgtcagcgc cacatattca gaatgtttaa
     5881 tctcctggca ttcttcagga ataccccctt ttatttccct gccataagtt cctactttaa
     5941 acaccacgca cagggcatca cgggagagtg acggggtcca ccaggagtcc gtgttaaaca
     6001 tggaggggat tctcattacc cccagctttt cacagagcca gacaaggaaa tcaggacgct
     6061 ttcttaactc gcgttcagcg gcttcaacca ttgcggcaat cgccggacct tcagggcatg
     6121 actcatcagg tctggcaata aagcacggtc cgcccccgat aaaagccaca tcatcgcgct
     6181 tacgccagcc cgcaggcaga gaaccagaaa aataaaactc actgattacg tccatggcga
     6241 acggctttaa gcgcaatccg gcagcgcctg tatttttcag taatgcccga agaatactgt
     6301 tgcgtagcgg taaaacatcc cgctgatatt cggttaaata tttatttgtg tacactatgt
     6361 ctaatttgaa ataaaaatat gccatgtttc cgattcctta ttttcagctt tcaggcgcag
     6421 gcagtcccct gacgcgagcg ccataattaa aacgatgtga tatttaaaga tttaatgtgg
     6481 tgttatttat tcagtccggc gtcctgttca aaaggctcga cataaaaact ttcagcgcct
     6541 ttattcacct ttataccggc aatgcctttc accgcatccg gctccgccag gacggcttcc
     6601 ttgttcactt cctctttcgt gcggatgaat cgctcaagcc ccataaggcg cagcatttca
     6661 atcacgcctt ccacatcacg gctgacgctg catgatggat ttcccagccg ccatgacacc
     6721 gtgccggtgg tcagattggc agtttttgtt ttgccgccgt tcgtcagctc atctcggttg
     6781 gttttgcacc agtcctgaat ccccttaaaa agcactttga tttctttttt aaggttttca
     6841 atttgcggcg tataacgggc ggtgatttct gccacttcat cattcatcgc cgtttccagc
     6901 cgcagcgcct ctcgctgaat atcgcccagg gtgcggatat cgcggctgac ctcttcgcgg
     6961 gtctgcggtg cagcctcggc tgcggccttt aattttgtaa cgcgttttgc cattttgttt
     7021 ttccttattg cagtgtgcct gatgctttat gcacgcggtg gcgtttaatg atggcatccg
     7081 ccccaaaagc ctgtacagat gcctgaaatt ccgctgagag aaactgaata atttcaggac
     7141 tgtgatactg gagaatactt
//
LOCUS       DAZUFB010000174         7105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZUFB010000174 DAZUFB010000000
VERSION     DAZUFB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.174"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..1449
                     /gene="adeQ"
                     /locus_tag="ACHY3M_004692"
                     /gene_synonym="yicO"
     CDS             115..1449
                     /gene="adeQ"
                     /locus_tag="ACHY3M_004692"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFS4319453.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1502..1954
                     /gene="yicN"
                     /locus_tag="ACHY3M_004693"
     CDS             1502..1954
                     /gene="yicN"
                     /locus_tag="ACHY3M_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFS4319454.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2165..3355
                     /gene="nepI"
                     /locus_tag="ACHY3M_004694"
     CDS             2165..3355
                     /gene="nepI"
                     /locus_tag="ACHY3M_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFS4319455.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQITFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAILWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3396..3689)
                     /gene="yicS"
                     /locus_tag="ACHY3M_004695"
     CDS             complement(3396..3689)
                     /gene="yicS"
                     /locus_tag="ACHY3M_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFS4319456.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            3911..4729
                     /gene="nlpA"
                     /locus_tag="ACHY3M_004696"
     CDS             3911..4729
                     /gene="nlpA"
                     /locus_tag="ACHY3M_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFS4319457.1"
                     /translation="MKLTTHHLRRGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4733..5656)
                     /gene="yicL"
                     /locus_tag="ACHY3M_004697"
     CDS             complement(4733..5656)
                     /gene="yicL"
                     /locus_tag="ACHY3M_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFS4319458.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSNALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5767..6951)
                     /gene="setC"
                     /locus_tag="ACHY3M_004698"
     CDS             complement(5767..6951)
                     /gene="setC"
                     /locus_tag="ACHY3M_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFS4319459.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1788 a         1596 c         1788 g         1933 t
ORIGIN      
        1 attattaaag aatatccatt aatgtgcaat tgaaatgtga taattatcac tgaaccgttg
       61 ttgtcatttg ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat
      121 aatgacaata ccgattacgt gagtaatgaa tcagggacgc tttcgcgatt atttaaacta
      181 cctcagcatg ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc
      241 atggtgtaca tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa
      301 gtggtgtttg ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt
      361 gctaacttac ccgtggcgct ggctccggca atggggctga acgccttctt tgccttcgtg
      421 gtcgtggggg cgatgggcat ctcctggcag accgggatgg gcgcaatatt ctggggcgca
      481 gttggactat ttttgctcac gctgtttcgt atccggtact ggatgatctc caacattccc
      541 ttaagtttac gtattggtat caccagcgga attggattat ttatcgcctt aatgggatta
      601 aaaaatactg gcgttattgt cgccaataaa gacacgctgg tgatgattgg cgatttaagt
      661 tctcacggcg tgttgttagg tattttaggg ttttttatta taaccgtgtt gtcatcacgt
      721 cattttcatg ccgcggtgct ggtttctatt gtggtgacgt cttgctgtgg attatttttc
      781 ggtgatgttc attttagcgg cgtctattcc attccgcctg atattagcgg cgtcattggt
      841 gaagtagatt tgagcggcgc gttaacactt gaactcgccg gtatcatttt ctcctttatg
      901 ctgatcaacc tatttgattc atcaggaaca ttaattggtg taactgataa agcgggctta
      961 atagatagta acggtaaatt ccccaatatg aataaggcgc tgtatgttga tagcgtcagt
     1021 tcggtggcgg gggcgtttat cggcacctcg tctgttactg cctatattga aagtacttct
     1081 ggtgtggcag tcggtggtcg cacggggctg actgcggttg tggttggcgt tatgttcctg
     1141 ttagttatgt tcttctcacc gctggtggcg atggttcctc cttacgcaac cgccggagcg
     1201 ttaatctttg ttggcgtgct gatgacttcg agcctggcgc gcgttaactg ggatgatttt
     1261 accgaatcgg tgcctgcgtt tattaccacg gtgatgatgc cctttacttt ctcgatcacc
     1321 gaagggattg cactcggctt tatgtcgtac tgcatcatga aagtatgcac cgggcgctgg
     1381 cgcgatctga acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa gattattctg
     1441 gtggattagt aactggcgag ggtttccaga tatcatgagt tctgattacg caggagaact
     1501 catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt ttcgggtgct
     1561 gacatccggg gccagaaaag cgattcgccg tctcagcgat cgactgaaca tcgatgtcgt
     1621 acccgtggag tcgatggtcg atcaaatggg aaagtcagct ggtgacgaat ttttacgtta
     1681 tttgcatcgt ccggatgagt cgcacctgca aaacgccgcg caggtgttgc tcatctggca
     1741 aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga ttttacaaaa
     1801 agctcgcctc gccgcgccga ttaccgacgc tcaggtcagg ctggcgctag gttttctgcg
     1861 cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata acgcgttctt
     1921 tcagcctgcc gagggcgttc actggttgca ttgaaaccgt ttcgtcttat cagcctacag
     1981 accgcgcctg acctgtcggg tggataagac gttcacgccg catccggtaa tctttgccct
     2041 gttccgtttc gatcttaact ccagctcaag caacaccgta aaagttgcaa aaacgttaaa
     2101 tgcgtcacac atttcaatgt taaagtggtc ggcttttccc ctgaaacatg ccacgggtaa
     2161 caccatgagt gaatttattg ccgaaaaccg cggcgcggat gccatcaccc gaccgaactg
     2221 gtcagccgtt ttctcggtgg cgttttgtgt cgcctgtctg attatcgttg agtttttgcc
     2281 cgtcagtttg ttgacgccaa tggcccagga tttaggcatt tcggaagggg ttgccgggca
     2341 atcggtgacc gtgaccgcct ttgtggcaat gtttgccagt ttgtttatta cccagacaat
     2401 tcaggctact gaccgccgct acgttgttat tttgtttgcc gttttgctga cgctctcctg
     2461 cttgctggtt tcctttgcta actcattcag tttgctttta atcggtcgtg cctgtctggg
     2521 gctggcgctg ggcgggttct gggcgatgtc ggcgtcgctg accatgcgtc tggtgccgcc
     2581 gcgtacggtg ccgaaggcgc tgtcggtgat cttcggcgcg gtttctattg cgctggtgat
     2641 tgccgcgccg ttgggcagtt ttttaggcga gcttatcggt tggcgcaatg tctttaatgc
     2701 ggcggcggtg atgggcgtgc tgtgtatttt ctggattatc aaatcattgc cttcactgcc
     2761 aggcgaaccc tcgcatcaga aacaaattac tttccgctta ttacaacgtc cgggtgtgat
     2821 ggcagggatg atcgccatct tcatgtcttt cgccgggcag tttgctttct tcacatatat
     2881 tcgcccggtg tatatgaacc tggcgggatt cagcgtggat ggcttaacgc tggtgctgtt
     2941 gagttttggc attgccagct ttatcggtac gtcgctttcg tcattcattc ttaaacgttc
     3001 ggtaaaactg gccttagcag gcgcgccgtt aatactggct gtgagtgcgt tggtgctgac
     3061 gttgtgggga agcgataaaa tcgttgctac cggcgtggcg attctctggg ggctaacttt
     3121 tgcattggtt cccgtcggct ggtcaacgtg gatcacccgc tcgctggccg atcaggcaga
     3181 aaaagccggg tctattcagg tggcggttat tcagcttgct aatacctgtg gcgcggcaat
     3241 cggcggttat gcgctggata atattggtct gacttcgccg ttgatgttgt ccggcacatt
     3301 gatgttgctg actgcattgt tggttactgc aaaggtgaaa atgaagaaat cctgatatca
     3361 ggtaaagaag cgttggtcag tagttgttgc tgttcttaca tatccgggca ttctacctta
     3421 cccaacgctt cccaataatt gttctgatta ttacgttcca tcgccacgat ggcctcgcgt
     3481 atatgttgtt tattgttttc atctgacaac attaactttt cccacgcttc atctgataat
     3541 gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt ctcttttgcg
     3601 gactgcagtg agctaaaagg cgactcggcg tatacgatgc ctggcatagc gaaaaaagtg
     3661 aaaataagca gtagtgtcgt tggtttcatg gattcaacct tttacaaatg tgtatcgaat
     3721 gaagcataag tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac gcgcttgcgt
     3781 ggtcagtaag aagtgccaga ctttatattc cacttttatt cctttttatt cttatcgata
     3841 gcgtttcgtt ttttaaaccg cagcgacctt accgctatag tcaggtaatc attaataaaa
     3901 ggataaaaaa atgaaactga caacacatca tctacggaga ggggccgcat tattgctggc
     3961 cggaattctg ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca ttaaagttgg
     4021 cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg caaaagagaa
     4081 atatggcctc gatgttgaac tggtaggatt tagcggttcg ttgctaccga acgatgcgac
     4141 aaatcatggc gaacttgatg cgaatgtctt ccagcatcga ccctttcttg aacaagataa
     4201 tcaggcgcat ggttataaac tggtagcggt gggaaatacg tttgtcttcc ctatggcggg
     4261 ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa ggcgcgacag tggcgatccc
     4321 caacgacccg accaaccttg gtcgcgcact tttactgttg caaaaagaga agctaattac
     4381 tctgaaagag ggaaaagggt tattacctac cgcgctggat ataaccgata atcctcgtca
     4441 tttgcagatt atggaactcg agggggcgca gctcccgcgc gtactggatg atcccaaagt
     4501 tgatgtagcg attatcagca ccacttacat tcagcagaca gggctttctc cggtgcacga
     4561 cagcgtattt attgaagata agaattcgcc gtatgtgaat attttggtgg cacgggaaga
     4621 taataagaat gcagaaaacg tgaaggaatt tctgcaatct tatcaatcac ccgaagtcgc
     4681 taaagcggca gaaaccattt ttaacggtgg cgcggtgcct ggctggtaat tctcactcat
     4741 gccgcgccgg acgatttatt tttctggcgc ggcgacggga atccattgaa atcaaaatta
     4801 ccgatgacag aattagcagc gttcccagcc agtccggtaa tgtgaacgtg atccccagca
     4861 acagcaaaga gagtagcgcg ttacttaatg gttctgcaca gctcaaaatg ctggcttttg
     4921 gaccgccaat taattgtgct cctttcaggt acagactaaa tgtcagggac gtaccaatga
     4981 cgatcaaata aaaaaacgcc agaatcaaac tgccgttaac gacaaagttt gttccttgtc
     5041 tggcataaaa aggcaacaga atcagaccgc caatcagcat actccagccg acgactggta
     5101 acgtgccata gcgggcaatt agcgttgagg gataggtggt atagaatgca gcagcaaatg
     5161 ccgaggcaat gccccagaac aacgcggcag gagagatcga taatgacgtc ggattaccgt
     5221 gtgtcaccaa taaaaaagtc ccgaccagcg atgtcaaaat agcgcagaaa accagaatgc
     5281 ccgggcgcga tttacgcacc agtgagaacc aggcgacgat aatcgtcggt gagaggaatt
     5341 gcagcaccgt tgccgtggct gcgttcgatt tttcgatggt tagcaaaaaa gtgagctgta
     5401 cagttagcgc gccaaccacg gaaaaaatca gcaggctaat ggcatcttta tgattgttaa
     5461 taatagaaaa gattttatcg ccatgaacaa atgacagcgt cagtagaatc aaaccggcga
     5521 atatcaaacg cgtcatagtc aaaaactgcg acgacatctg gctttgctcc atgatgtatt
     5581 gcgcacagac ccctgaactt ccccacaaca cggcggcaat cagaacgttc agcatcccct
     5641 ttctggtgga acccattttt ccctctgcat actgttttgt cgtttatgta tgcaggcatg
     5701 atagcaaaaa tggcgaggat ggctctgttt gcgccacgcc gatttttctt agcccagact
     5761 ggtgagctaa atatctttaa taaacagcag gcaaatcatc gcaataccca acatccctat
     5821 cgccagccag aacaacgcat ggtagctcca gatttcaacc ataattccgt caacagagcc
     5881 ggcgataatc cagccgacgc gtgaagtatt tgcatataac gttgtcgcag agcctatttt
     5941 ttcaggcatc aagtcctgaa aataaagcat gccgatacca cagagaatac caaggaagat
     6001 ggcattaaga atttgcaatt ccagctcaac cgccggagtc gtcgccatga gtacgctggc
     6061 gtaaaaacac attccactca cgatagcaat gagcattaat agtcgcttgc caatacgttt
     6121 catgtaatag cctgcgatta acatcatcgg aatttccaga ccggcagcga taccaatcat
     6181 ttctccagcc agtttatcgg ttagatgcag ttcatcaata ataaatagcg gcatatttat
     6241 catgtagaga ttattcgccg cccacatcat tgaacagacc acaaaaagta gccgcgtatc
     6301 ccgcttcctg tgggtggagg gtaaaatttc tacgggttgg gtaacgacag gaatatttct
     6361 ttgtattgat ggcaaaaaca accagactat cagtccgcaa acaacaaatg cgatggcagc
     6421 ggtgagatac atcactttaa aactaaatcc cattgccagt tcataagcga gcggtggccc
     6481 gataacccag gcaagcgaga tctgagcacg taaaaatgta ctgaacatga ccgtctcacg
     6541 gcctgttctg tcggcgtgtt cacgggcgag ggcgaacatt tgcgggtttg cggtggaagc
     6601 aaaactactc agaagtacgc ccgttgagag gagaatgaag tagttgcgat tccacgcaaa
     6661 aagcgtgcag gccagcactc caaataagca acatagcaga atcagtaatt tacggtcgcc
     6721 ttgtttatcg gagtgccttg ccagaaattg actgaccaga attcccataa tagcgctacc
     6781 ggtgaagaaa aaatctacca ttataggacg ggctttcagt tcatctccga ggaatatact
     6841 tagggtagga gtctgaagag cgcccgcaat acccgtcaga aaggcgacaa gtaaaaatgc
     6901 cgcggcagtg agatcaagta tttttgatgg agtggtagcc gttttttgca ttcttctgaa
     6961 tcaccgggcg acaaaatata actgagttac aaataagtac cagtcgaatg agtattgtat
     7021 taagaatttt tgagtatcgc tgatacaacc agaaagcaat gctagcgcaa tattataggg
     7081 ccagcaattg agattattag caaaa
//
LOCUS       DAZUFB010000175         7016 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZUFB010000175 DAZUFB010000000
VERSION     DAZUFB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.175"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..625)
                     /locus_tag="ACHY3M_004699"
                     /pseudo
     CDS             complement(92..625)
                     /locus_tag="ACHY3M_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(630..794)
                     /locus_tag="ACHY3M_004700"
     CDS             complement(630..794)
                     /locus_tag="ACHY3M_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319460.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(791..1525)
                     /locus_tag="ACHY3M_004701"
     CDS             complement(791..1525)
                     /locus_tag="ACHY3M_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4319461.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KRVYTWRVADNNVQQEQPAPEETTTATEAKISECDLTATIEQRGPQTADELATLFGTT
                     SRKVASTLAMAISKGRLIRVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGV
                     ALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKW
                     ERVCAALRELNKHRDMVAGICRKSGQ"
     gene            complement(1559..2020)
                     /locus_tag="ACHY3M_004702"
     CDS             complement(1559..2020)
                     /locus_tag="ACHY3M_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS4319462.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(2013..3053)
                     /locus_tag="ACHY3M_004703"
     CDS             complement(2013..3053)
                     /locus_tag="ACHY3M_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4319463.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3025..3576)
                     /locus_tag="ACHY3M_004704"
     CDS             complement(3025..3576)
                     /locus_tag="ACHY3M_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4319464.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3560..3786)
                     /locus_tag="ACHY3M_004705"
                     /pseudo
     CDS             complement(3560..3786)
                     /locus_tag="ACHY3M_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
     gene            3863..4270
                     /locus_tag="ACHY3M_004706"
     CDS             3863..4270
                     /locus_tag="ACHY3M_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319465.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4535..4834
                     /locus_tag="ACHY3M_004707"
     CDS             4535..4834
                     /locus_tag="ACHY3M_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319466.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4907..5125
                     /gene="ydfC"
                     /locus_tag="ACHY3M_004708"
     CDS             4907..5125
                     /gene="ydfC"
                     /locus_tag="ACHY3M_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFS4319467.1"
                     /translation="MQKRAPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5148..5555
                     /locus_tag="ACHY3M_004709"
     CDS             5148..5555
                     /locus_tag="ACHY3M_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319468.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5533..5766)
                     /locus_tag="ACHY3M_004710"
     CDS             complement(5533..5766)
                     /locus_tag="ACHY3M_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319469.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            6327..6515
                     /gene="dicB"
                     /locus_tag="ACHY3M_004711"
     CDS             6327..6515
                     /gene="dicB"
                     /locus_tag="ACHY3M_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4319470.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            6512..6700
                     /locus_tag="ACHY3M_004712"
     CDS             6512..6700
                     /locus_tag="ACHY3M_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS4319471.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            6796..>7016
                     /locus_tag="ACHY3M_004713"
     CDS             6796..>7016
                     /locus_tag="ACHY3M_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4319472.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDS"
BASE COUNT         1780 a         1790 c         1547 g         1899 t
ORIGIN      
        1 gataaactta tccgacttta cctcgtgcat agcttgttat ttaaagtcaa caaaataagg
       61 aaaattatac gcatattgaa gagtataaac cttacatgtt gattacattt ttgtaatcaa
      121 catcctgttt ggaatagcca gcctttaatg gataactatt tctgacaatg caatgagtat
      181 aatcaagtcc atcttccact gagaattaga ggcggcatgc tttttccggc tcttgccgga
      241 tatccgtaat tgtccataat ctgcagattt ataccttctg catgacctgt cagcgaaaat
      301 ttgtccggtg tttctacggg aatgacatca aaagttacac gcactcgcgt taccgtgtag
      361 acctactttc ctgcacttgc aagatcacag tggtgtaacc gtaacaggaa tttattctct
      421 ggaccggcag taaatccctg agtggcgtgg ttcccatatc aatttcccgc caggcagcct
      481 ccattgccag cgtacaggct ggagccatga cctgcccttt aaatctggcc cgaccatccc
      541 accggacgtg ttcttctccc ctgaacttag gtacagtcat ctccagtggc acaaaagtgt
      601 cagcgccata atttttgacc gttatcgcgc tacggatatt ttgttgactg gtgaaaatca
      661 ccccgcagaa tcaggcttat tcccttaacc tgggctttca tcctgaccgc cgcctcacta
      721 cgaccaatca gactgccgat gcattttacc ttcattgtta gtatcatgat ttcaggcctg
      781 caccatccgc tcattgcccg gacttccgac aaatcccggc aaccatatcc cggtgcttgt
      841 tcagctcccg cagcgcggcg cagactcgct cccacttctg gacatgattc ttcgccctac
      901 gcagttcgcg atttgccata tgcagcgatg gcaaaatcaa atcatcttct cgcgttgcag
      961 taaacgatgg cagcgactgc acaatgtccg ccacagtttc tgttttaata tcttcctgtg
     1021 ttgcatcttt ctgtaccggt aacgcaacac cggctggctg aggaaaggct ttaccatcgg
     1081 tttccgctac cgattcaact ttcggctctg ctggtaaatt atcacctggt atgcagtaac
     1141 gaattttacc gttctggttt acgcgaatca gacgaccttt gctgattgcc attgccagcg
     1201 ttgaagcaac tttgcgggat gttgtaccga acagcgtagc cagttcatcc gccgtttgtg
     1261 gtccgcgttg ttcaatcgtc gcggttaaat cgcactctga gattttcgct tctgttgccg
     1321 tggtggtttc ttctggcgct ggctgttcct gctgaacgtt gttatcagcc acacgccagg
     1381 tgtatactct tttatcaacg aagccagcct ttttcagttc ccacagctcg ttcagtactt
     1441 cttcacgact gatatcaagt cgcgcagcca gctctaccga cgtggctttt cccatcgctt
     1501 tcagtgcgtc aaaaacagtc tccataaatt tcctcccggt aaaaaatcac ttctcaactc
     1561 aaacaaaacc agccgctttc cggcgttcat attcctgttt cagcaactca attggcgttg
     1621 gccccgacgg gcgtttgggg gccgccagtt gtcgccggac tggcggaacg ctcaggccgt
     1681 tactaacatg ctttgcccat ttcgtcagtt gccgttctgc aagccgtttt aattcccctt
     1741 cggtcatctg gcgctcaatc ccctttgaac gcatctcgag gcaaatgtga tacagcacag
     1801 gctgagacca cgggtactta tcacttccgt cgtatcgcca ggactcattg cgccagcggc
     1861 ggtactcctc catcacagca tccaccgtca gaccgaatgg attggctccg cttttcgaaa
     1921 tcagtgccac aaactcagcc aggtccggag gccatgtttc acccgcccgg cagcggtcca
     1981 tgcactggcg gcagacctgc cggatttgct gctcagtcat cgcgccaatc tgtgcaatcc
     2041 agagcttcga aggtgcggcc ccgttcttct ggatccagcg gttcgaataa accaccccca
     2101 tgagttccca cagcttccag gccgtttccg tcgctgataa atccgttttc acgttcccac
     2161 tgctcacgtg ctgcccgaat ttcctgaact gcccgtgatg cggtgccacc tggtgctgct
     2221 gcatggttta cccctttgct gactggttta acctgcgccc tgacgtgatt tacgtgacgg
     2281 gcgaatttct gctcccactg aatctgcgta aacactttcc cctccgctgc ccagtagtcc
     2341 cggaaggcgg caagttcagc aggtgtaaat tctgtctccg gcaaagccat cccccacaac
     2401 gcagcccgtc gtcgaaaatc ccgtgacgga taccagctat cgctcatcgg gaattttccg
     2461 atgggttcgc tcaggccata caggaataca gggggggctg cctgtaacga caaaacttcc
     2521 tgctcactgg tcggagcact ctcgcgtgcg ttatgtgtgg ggtttagatc tttgggttcc
     2581 tttgggttcc gtgatccgtt tttgggtgtc tttgatggaa aatttgggtg tctttggtta
     2641 ttttccatgc agctaagagt tctgtttttg ggtctgtttt gtgctgaaac ataaccattt
     2701 tcggtactgt ttttattaac agcaccaatt ttacccacct ttaaagactc ccgtttttgg
     2761 gtgtattcag gctcggcaac actttcttct acaccgataa gtcggtacac cacaatttgc
     2821 tttgttctgc cttttctctc accggtatca acaattaacc caatctccat caggtgtcgt
     2881 aagctgtcct gcacagtctt tttgttcagt tccgttactt ctgccagtgc agatacagac
     2941 gggtatgcac acaaatcggc accgcacata tcagcaagcc aggtcaatac tgacttactg
     3001 gatgaactgc cggttttcac ctttttagcc catcgtagtg catcgatact catacgaacc
     3061 cctggcagac atttgtttat ctgcaaagta atattggtat tgctgacgat acgcgtgctt
     3121 gaaagcaata gctttttcta taagctcgtc agtctcacgt tccacaacaa ctggatccgc
     3181 aaaaagcagc ccggactcca ccacatcgcc atattctttg tttaacccgg cgatcatgta
     3241 cgtaatgctt tttccgtcag taatttcaca atacaatctg aaatcgctga tccggatagc
     3301 ctccataatt gccggaatca gcgccgtgaa tttgtcacgc ttatctctgg tgtcgatagc
     3361 tttccagcgt tcgaatatct tcacccggtt aacgcccagc gcccgttgat caacctcgcc
     3421 atcattaaac gtgacgcgtt gaacatcgat gttcgggcgt tctttcagag cccagaatgc
     3481 ttccgtgatt aatatcgtcg cctgctcctg tgtcattcct ggtcggcata cccaggtatc
     3541 cagagcctca caaacctgtt cagtggtgat tttcattgtt caaccgcccc gcccgctttg
     3601 ccttacgata ttcgtcataa actttggggt cgtactgaag ttccccgccg gatgcctctt
     3661 gcaggcgcat cgcgcgacct tcaggaacca gttcccccca tgcagcaacg cttgccagcc
     3721 taactcctgc gacattggca agctttgttt tgctgccaaa aacgctatag catcaatttt
     3781 caacatatcg aaccccttag attttcctaa ggaaactaga tcgtagagaa acctaagtca
     3841 agaaaaatta gaattaccta atatgaaaaa cgaaaccttc ggtgctcgcc tcttatacag
     3901 gcgaaaaaaa ttaaaactgt ctcaggccgc attaggtaag ctggtcaaag tggctcacgt
     3961 aacaatttct caatgggaaa gagatgaaac acagccagcg gggaagagat tattcgcact
     4021 gagccaggcg cttcagtgct cgccgacttg gcttcttttt ggagatgaag ataagcaacc
     4081 aggcgaaccg atcccagata atcagccagt caatctgaca gaagatcaaa aagagttgct
     4141 tcaactgttc gacgcactgc ctgagtcaga gcaaaaggct ctgttgtcag agatgcgtgc
     4201 tcgagttgag aatttcaaca aactttttga agaactactc aaagctcgca aaagaagcgc
     4261 aaataaataa cccccctttt tttcgcaact ctctgtaata aaaagcacaa actttcaaat
     4321 acttgtgttt tttacatcaa gaagcttagg tttttctaca caaaaagctt gaccataact
     4381 cttaggctat tctaaattct actcatcaag acaccgcacg gtgttctcag caaacagttc
     4441 cgctacccgg cgttaagggg aagcagagga tttctcagtg ggcgaagtca aacatcagaa
     4501 tggaaggcgt ccagggatca gcaaagaaac agcgatggcg ctttatattg atatcagcgc
     4561 cattgccgga caggtaagag ttatcagagc ggtaactaag cggtatgcgc ctttacttca
     4621 gaaagtctct ggtgagtgca ccgaagatat tgtcaacgat ttcgtcatcg aactgcgagg
     4681 actcatcttc agttacaagg tgaccacaat ttttgcagat ggctcccgcg aaactgtcag
     4741 agccctgcgg cttaaaggat gtgtcaaaga cttagccacc acattctggg caagaaaact
     4801 tgattgtatt cataaccaat ttcctctcga gtaacagacc cctcagagga taccacctcg
     4861 cctgacgtgg ttaaaaagca ggcaacgcta accacaagga gccgacatgc agaaacgagc
     4921 ccccgtcatc atcgcgccag actataccga tgatgaactt tatgagtgga tgcaccagaa
     4981 aattaaggcg gtgcaggacc tgaaatgggc caatgaagcc agggctaagc aggctgaaaa
     5041 tctgtccgct ctggagcagg atatcaccaa tctggaaaaa gcagcggcat taagcattgc
     5101 cagaatgatt acatacccac gttaatagct aaccaacgag gctaataatg gaatttaaag
     5161 atttaccaac gccattacag gaaatggcat cgaatatagt tcgctcacaa ctggctactc
     5221 ttgaccttag taccgcagaa aaagaaacca tcgataatat ggttcgtaat gtgcgcaatg
     5281 ctttttctgg gctatatggt tctgataatc aaaagcagga aagcgatgtt aataaacggg
     5341 taatttctgt ttgcgtgaat ggccatgttc tttcatcaat caaaacagaa acggcgacag
     5401 tcttcgattg cctttgcatt gtacagagcc ttgttgatgc cctgtttcgt tcagtgaatt
     5461 tagaaaatga tgcaaatctg cgagggcgca taatagcaca tccatatgca catactttag
     5521 gctctgtgga tatcaaagat cccacaaatc tttaatgaaa tagttaacgc gaattgtact
     5581 tgctctttcg gttgctttca gaatacgcgt tgaaactgct ggcggtaatt tggtattcca
     5641 tttattaaaa tcatgcccgg gaaagtactc ttcgaaaata cttttaactg cagactcgcc
     5701 tattgaaatg ctgcttacca tgcgattttg ataaaggcat ttagcaataa gagttgattt
     5761 taacattcac cctcctgagg gttggtaatt aaggagttct ccacgggtga agtggagtgc
     5821 gtgcgccgga cacgggtgaa catccggcac tgacagttta ctgaaaggat atttctctga
     5881 aaagtcagac cataacgcga aagcgcacgg cgaggtagct ggttcataga tagcctgtcg
     5941 ttaaattttc gtcgaccgtg cgcttccggt tgtggcaatc cgcgaaatgg cgcgtcggta
     6001 agtatggagg ggttattcct tccccgttga ggacaccggg ttgtcaggtt gaccatacgc
     6061 ttaagtgaca accccgctgc aacgccctct gttatcaatt ttctggtgac gtttggcggt
     6121 atcagtttta ctccgtgact gctctgccgc cctttttaaa gtgaattttg tgatgtggtg
     6181 aatgcggctg agcgcacgcg gaacagttaa aaccaaaaac agtgttatgg gtggattctc
     6241 tgtatccggc gttaattgtt aactagttaa cgtcacctgg aggcaccagg caccgcatca
     6301 caaaattcat tgttgaggac gcgataatgg aaacgttatt accaaacgtt aatacgtctg
     6361 aaggttgttt tgatattggt gttctgctca gtaaccggga gtttacggaa gatgccatta
     6421 agatgagaaa atatgaacct tatcttctca atgataattc catactttcc agaattgccc
     6481 ttcttgaact tggtattatc ggagaacagc agtgacttca gcatttgcac tggtgatgac
     6541 cgtttttctt ataacgggtg agccacaaaa tgtgattacc ggaatttatg acagtaagtc
     6601 atcctgcatt caggtaaggg acgaacaaaa aatccccggt gaatgcctcc cgttaaaaaa
     6661 agtatcgctg aacctgaata acgaaatacc ggctggataa cccgccagcc atattaacgc
     6721 cataccaacg gattaaaaat gccagcaatg gcagggattc gttcaccctg aaatctgtaa
     6781 tgaggttaaa acaaaatgag taaagtcttt atttgcgccg ccattccgga cgaacaggca
     6841 ataaaggaag aaggtgccgt cgctgtagcc actgccattg aagccggtga tgaacgtcgc
     6901 gcccgcgcaa aatttcactg gcaattcctg gaacattatc cggctgctca ggactgcgct
     6961 tataaatttc ttgtctgcga ggataaacct ggtatacccc gccctgccct cgattc
//
LOCUS       DAZUFB010000176         6856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZUFB010000176 DAZUFB010000000
VERSION     DAZUFB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.176"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(281..766)
                     /gene="sixA"
                     /locus_tag="ACHY3M_004714"
     CDS             complement(281..766)
                     /gene="sixA"
                     /locus_tag="ACHY3M_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFS4319473.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(969..3113)
                     /gene="fadJ"
                     /locus_tag="ACHY3M_004715"
     CDS             complement(969..3113)
                     /gene="fadJ"
                     /locus_tag="ACHY3M_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFS4319474.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3113..4423)
                     /gene="fadI"
                     /locus_tag="ACHY3M_004716"
     CDS             complement(3113..4423)
                     /gene="fadI"
                     /locus_tag="ACHY3M_004716"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFS4319475.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMNEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4603..4887)
                     /locus_tag="ACHY3M_004717"
     CDS             complement(4603..4887)
                     /locus_tag="ACHY3M_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFS4319476.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5259..6599
                     /gene="fadL"
                     /locus_tag="ACHY3M_004718"
     CDS             5259..6599
                     /gene="fadL"
                     /locus_tag="ACHY3M_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFS4319477.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1705 a         1841 c         1622 g         1688 t
ORIGIN      
        1 atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt
       61 aataaatgac aatgagtgtc aaacagacat tattcatttc aacatgtaac tttgggcaaa
      121 atcaagatac gcgcagaaga ataatttccg aatgaaagta gaaagtgttt tatttataga
      181 tggggagagt cttcgatagt agtgaggttg acagatatcc gctgcctgat gagcaattat
      241 gcccaatgaa tctacctcat tgggcataat ttggaactca tcagatagct tttgccatct
      301 tcagattaca cgggctcatc tgccagttga atgtgccgtt tccgctttca tcaagcgtta
      361 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
      421 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
      481 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
      541 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
      601 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
      661 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacg gaatcactgg
      721 cggcatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
      781 gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
      841 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
      901 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
      961 tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
     1021 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc aagtcgttgc
     1081 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
     1141 aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata
     1201 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
     1261 acctgcggtg cggagagtcg cccctgccct tgtgcgccaa tgagcgggta aatggcggga
     1321 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
     1381 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
     1441 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
     1501 tcatccaaaa gttggattgg acctaccgga aaaccaaatt tcactagcgc ggcatcaatg
     1561 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
     1621 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
     1681 ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc
     1741 tctaccagcg gcattttttc caccggactg aagaaatgca ggccaataac ttgctcaggt
     1801 cgcgtggcgt gagctgcgat atcaccaatc ggtaaagatg acgtattcga agcaaaaatg
     1861 gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt
     1921 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
     1981 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
     2041 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
     2101 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
     2161 aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc actaccggga
     2221 tctttcttca cttccgtact ggcaaaaaag atactacgca aagcttgcga ttgtggcgtc
     2281 atcgccagtt cgccaaacgc ccgagcttcg gcgtcataac cgctgctggt gccctgcgct
     2341 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
     2401 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
     2461 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
     2521 gcgacttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
     2581 tgtttcgccc gaagttgttt tccggtgagg atcatctcta atgctgtgct gacgcctatc
     2641 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcaggc
     2701 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccaac
     2761 tccagcccac cacccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
     2821 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
     2881 ttgcagttgc cgatcatgtt gatgtctgcg ccagcaatga agttgtccgg tttagcggag
     2941 acaaacacca cgcctcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
     3001 tgcgaggcaa actccgcctt cagggtattc attttctcac ccggtacgtc gatggtgata
     3061 acggcaatgt tgtccagacg aacattaagg gtaaacgctg atgtcatttc cattattccg
     3121 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
     3181 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgtgcg ccggtcgccg
     3241 cgaagggatg tccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
     3301 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
     3361 gaatattcgc cagtgtctga gctgcaaagg cttcgttcat atcgatcaat gtcagatcgc
     3421 tcattgtcaa accggcacgt tccagcgcca gcggtgttga ccaggccgga ccgagcaaca
     3481 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
     3541 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca
     3601 gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
     3661 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
     3721 tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct
     3781 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
     3841 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
     3901 ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcagtcgct
     3961 ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt ttactgacgc
     4021 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
     4081 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
     4141 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
     4201 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
     4261 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgcgggaa
     4321 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
     4381 tacgatcgcc ctggcgggta accagcggta aaacctgacc cataacactc ccctgtaaaa
     4441 aaataaataa agtggtctga cctgatcata gtcttaacca tttttttaca tttagccaag
     4501 cggagaaaaa ggaaagtggg agctatgaca cagtgaagac aggagaaaga aaagaaaacg
     4561 cccctgccag caactgacag gggcgtacgt acccgagagg aattaacgca gacccagctg
     4621 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
     4681 agactcttca gtgaaagtcg gggtgatttt acacggttcg gattccacgc tacgggcttt
     4741 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg cttgcaaaca cgcggctgta
     4801 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
     4861 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
     4921 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
     4981 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
     5041 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
     5101 gaaaaaagat ggaaacatac ttgcaacatt ccagctggtc cgacctatac tctcgccact
     5161 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
     5221 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
     5281 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
     5341 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
     5401 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
     5461 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
     5521 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
     5581 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
     5641 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
     5701 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
     5761 gtcttggttt caacgccgtc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
     5821 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
     5881 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
     5941 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
     6001 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
     6061 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
     6121 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
     6181 ttgatccaca gtgggcgatt cactatagct tggcttacac cagctggagt cagttccagc
     6241 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
     6301 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
     6361 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
     6421 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
     6481 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
     6541 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
     6601 aacgcgttcg cctggataaa gtcacctgca tagcaggtga ctttaactcc cccacttcac
     6661 cgaaagtagt gtcctcattg cttacctcct gagttttgca aacagcctga tggcagcttg
     6721 ccctttcaaa tcaataagcg gtatccacag acaaatctga agggaaaggc atattttcag
     6781 gcgttctgct cgtccttctc aaagagttta cttttctgaa tttccaggat actcaccccc
     6841 cccctggcta ttgtgc
//
LOCUS       DAZUFB010000177         6818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZUFB010000177 DAZUFB010000000
VERSION     DAZUFB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.177"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..924
                     /locus_tag="ACHY3M_004719"
     CDS             <1..924
                     /locus_tag="ACHY3M_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFS4319478.1"
                     /translation="RLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGID
                     ATMTAIGVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSY
                     KTDISGRLPKGSAESLTVSGQPEHKVEAKDSNGMPVDNRQGTITVSASGLQVGDAFTI
                     AGVNSVHQITKDTTGQPQVFRVLAVSGTTVTISPKILPVENTDVASRPYANVDAKPAA
                     SAAITILNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFD
                     HIKGVTTARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            966..1154
                     /locus_tag="ACHY3M_004720"
     CDS             966..1154
                     /locus_tag="ACHY3M_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319479.1"
                     /translation="MARTMLYKSGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            1135..1596
                     /locus_tag="ACHY3M_004721"
     CDS             1135..1596
                     /locus_tag="ACHY3M_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFS4319480.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            1606..3024
                     /locus_tag="ACHY3M_004722"
     CDS             1606..3024
                     /locus_tag="ACHY3M_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFS4319481.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQSAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKRVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3024..3872
                     /locus_tag="ACHY3M_004723"
     CDS             3024..3872
                     /locus_tag="ACHY3M_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFS4319482.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3872..4327
                     /locus_tag="ACHY3M_004724"
     CDS             3872..4327
                     /locus_tag="ACHY3M_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFS4319483.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQIYCVMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4330..5025
                     /locus_tag="ACHY3M_004725"
     CDS             4330..5025
                     /locus_tag="ACHY3M_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFS4319484.1"
                     /translation="MLYAFKLGRKLRGEEPWCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQFNTIMNNLKPFTPLAEKYVGSLENLSSLEWQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQQWLSGQMNNYQNLANIGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANRPSGLQSAIGGAASGAIAGAQLGSIVPGIGTGIGA
                     AVGGGLGLLGSLF"
     gene            5036..6400
                     /locus_tag="ACHY3M_004726"
     CDS             5036..6400
                     /locus_tag="ACHY3M_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFS4319485.1"
                     /translation="MATWQQGINSGGFLAGIGAQNENAPKASDINATLGLIRENNELA
                     RSGANNVGLTALRGLAGVADIYKQQQQQERKAAFQKGYADAYASGDREQMRNLITAFP
                     EEFEEVRKGMGYVDDAQRDDFGNLALKAQVASSLGPGAFGRFMMDSEKEMRRLGIPPE
                     TIAEMQVNDPQGFQHFAGNLALFSLGHEKYFDIKDRMEGRRLEGERNQLTARGQDITM
                     RGQDLSAATARRGQDLAMQRASMKGAVGNNERTVQLADGRTVTVGGKLHGAGANAFYE
                     GIDNEGNMVRVPAGSIAAPATSAASAQNYAMKKDLDAISGASIDDLGFMTGITGSSGS
                     PALGADIRSRASGGDQRKLYNAAQRIQGKMQNQGIAAARDMGASGINTVAEAKMYFQG
                     MPQVDFSSPEALQQSMRDIQQYTDNYNQQYNVNVGKSQRQQSQPTQVSQPAASSNFSS
                     LWGD"
     gene            6400..>6818
                     /locus_tag="ACHY3M_004727"
     CDS             6400..>6818
                     /locus_tag="ACHY3M_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFS4319486.1"
                     /translation="MAKAWKDVIASPQYQALTEEQKAQAQAQYFDEVVAPKAGDKWAE
                     ARDQFYAAYPPPQQQKEEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGA
                     SLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGETIG"
BASE COUNT         1781 a         1635 c         1798 g         1604 t
ORIGIN      
        1 cgccttgctg cacagattga ctctgacctg atttccatgg tcacgcagcg tgctactaac
       61 gtgatcacga tggctgactc aaccacaggt tcacagggcc gtgatttgtg gaactgtgcg
      121 gcaggtattg atgccaccat gacggcgatt ggtgtaccgc agggtatcaa ccgtcgctct
      181 ttctggaacc ccttcaacta caaagacctt gctggcgagc ttggtcaccg tgcctacgct
      241 cagggcgcaa ccctgacagc atacgaaaaa gcgcagatcc ctccggttgc ttcctttgat
      301 agctacaaga ccgatatttc tggtcgatta ccgaaaggaa gcgctgaatc cttgacagta
      361 tcaggccaac ctgaacacaa ggttgaagcg aaagattcaa atggtatgcc agttgataac
      421 cgacagggga ctattacggt atctgcatct ggcttgcagg ttggtgatgc gttcaccatt
      481 gccggtgtga attccgtaca ccagatcaca aaagatacca ccgggcaacc gcaggtattc
      541 cgtgttctgg ctgttagcgg aactaccgta acaatctctc caaagattct ccctgttgaa
      601 aataccgatg ttgcgagtcg tccatatgca aacgtcgatg ccaaaccggc agcatcagca
      661 gcaatcacca ttctcaacaa gaacgcagca cctgctaacc tgttctgggc tgatggttct
      721 gttgagctga tgtacggcaa actggcgttc ccgactggtc agggtccaca ggtaatgaca
      781 gcaaccaccg agcagggcgc tacgctgatc atgtcttacg ccttcgacca catcaaaggc
      841 gtaaccactg ctcgtttcac cactctgtac ggttgctctg tacttgttcc tgaatatacg
      901 ggcatcgtta ttgccgggca gtaattttgg tggggcttcg gccccatttt tattgggaga
      961 agacaatggc acgaacaatg ctctataagt cgggcaacat gatcacctgt ggtcagtttg
     1021 ctgtcgatta catcattgtt gatgacgaag aagttaaatc tcacctgaaa aaaggctggg
     1081 taaaaacccc tgaagaaacc gcaacgaagc aaaaagtggc taaggcggaa gaagatggcg
     1141 aaaacgaagg gtgatctcgt tcttaaggct ttacgaaaag ctgggctgta ttccaatgcc
     1201 acgttgacag atgctgaccc tcaggcaatt gaagatgcca ttaatgacct cgaagacatg
     1261 atggcagcat ggcaggctaa aggtatcgag cttgggtatc agtttgctga tacagaaaac
     1321 ggcatcatgc cgttacctga cgatgattca ggtatccctg catgggcaaa tgatggcgtc
     1381 gctttgaaac tcgctgtgca agtgtgcatg gataacgtca ttcagccgtc agacgctctc
     1441 cttaccgctg ctgacagtgc atatcagaca atctgtatcg ctttaaccaa aataccacca
     1501 cttgagcggc gaaatgacat gcctcgcggt agtggtaaca aaagcgcgtt tacgtggaat
     1561 cggttttaca tcgagaaaga tgatccgagt acgtgaggtg aataaatgcc gattcagcaa
     1621 cttccgctta tgaaaggtgt cggcaaagac tttagaaacg ccgactatat cgactatctg
     1681 ccagtgaata tgttggctac acccaaagaa atcctgaaca gcagcggata tcttcgctca
     1741 ttcccgggca ttgccaaacg ttctgatgtg aacggcgtat cgcgcggagt tgagtacaac
     1801 atggcgcaga gtgctgttta tcgcgtgtgt ggcggcaagc tgtacaaagg agaaagtgaa
     1861 gtcggtgatg ttgccggaag tggtcgtgta tcaatggcgc atggtcggac atctcaggct
     1921 gtaggcgtta atggccaact ggtcgagtat cgttatgatg gcacggttaa aagagtctca
     1981 aactggccta cagacagcgg attcacgcag tatgagttag gttctgttcg tgacattacg
     2041 cgcttacgcg ggcgttatgc gtggtcaaaa gacggcacgg attcatggtt tatcactgac
     2101 cttgaagacg aatcacatcc tgaccgctac agcgcacaat atcgtgccga gtcgcagcct
     2161 gacggcatca tcggaatcgg aacatggcga gacttcatcg tctgctttgg ttcatcgacg
     2221 attgaatatt tctccctgac tggtgcaacc accgttggtg ctgctttgta tgtcgcccag
     2281 ccatcgctga tggtgcagaa aggcattgcc ggaacttact gcaaaacgcc gtttgctgat
     2341 tcctatgcgt ttatcagcaa tccagcaaca ggtgcgccgt ctgtatacat catcggctcc
     2401 ggtcaggtgt caccaatcgc cagcgcgagc attgagaaaa tactacgctc ctacactgct
     2461 gatgaactgg ctgatggcgt gatggaatcg ttgcggtttg atgctcatga gttgctgatt
     2521 attcacctgc cgcgccatgt tcttgtttac gacgcatctt caagcgctaa tggtccgcaa
     2581 tggtgtgtgc tgaaaacagg cctgtatgac gatgtgtacc gcgctatcga cttcatttac
     2641 gaaggcaatc agataacgtg cggcgataag ctggagtccg tgaccgggaa attgcaattc
     2701 gatatcagta gccagtatgg gctacagcaa gaacacctgc tgtttactcc actgttcaaa
     2761 gcggataacg ccagatgctt tgatctggag gtggaatcat cgaccggcgt tgcgcagtac
     2821 gccgaccgcc tttttctctc tgcaaccact gacggcataa attacggtcg tgagcagatg
     2881 attgagcaga atgaaccgtt cgtttacgac aaacgcgttt tgtggaagcg agtagggcgc
     2941 atcaggaaaa atgtcggctt caaattgcgc gttatcacga agtcacctgt cactctgtct
     3001 ggcgctcaga taaggattga gtaatggcgg attcgaatct caatgtgccg gtaatcattc
     3061 aggctacacg gctcgacaca tcagtccttc cacgcaatat cttctcgcag tcgtatctgc
     3121 tttacgttat cgcacagggt actgatgttg gtaacgtggc taacaaggcc aacgaggccg
     3181 gacagggcgc ttatgatgca caagtcagga acgatgagca ggatgtgatt ctcgctgacc
     3241 atgagcagcg aatttctgct gcggaagcaa cgcttgttaa tcatgaggag cgaatcagcc
     3301 aggcagaatc aactcttcag gaacatgaaa cgcgaatcgc tcagaatgaa agcgatattg
     3361 cgtctcttga taccagagtt cagtcgctgg agtcgcaggt ttcagaccat gaaacgcgca
     3421 ttgatgctct ggagtatgca accacacgca agaagtcaga ggttgtttac tctggtgtat
     3481 ctgtaaccat cccgacagcg ccgaccaacc ttgttagcct gctgaaaacg ctcacgccgt
     3541 catccgggac gttggcacca ttcttcgaca ccgttaacaa caagatggtt gtgttcaacg
     3601 agaacaaaac cttgttcttc aagctgtcga ttgtcgggac gtggcccagc ggaaccgcca
     3661 acagatcaat gcaactaacc ttttccggct ctgttcctga cacacttgtg agcagtcgca
     3721 atgcggcgac aacaaccgat aacatcctgt tagctacgtt cttcagcgtg gataaagacg
     3781 gctttcttgc cacaaatggc agcacgttaa ccattcagtc aaatggtgcg gcgtttactg
     3841 ccacaaccat caagataatc gcggagcagt gatgattcag ttcaaaccaa cgcgaaacat
     3901 cgacctgatc gaagcagtcg gaaatcaccc tgacattatc gctggtagca acaacggtga
     3961 tggatacgac tacaagcctg aatgccgtta cttcgaggtg aacgtgcacg ggcagttcgg
     4021 cggcattgtt tactatcagg agattcagcc gcttacattc gattgccacg ccatgtacct
     4081 gccagagatt cgcggcttca gcaaggaaat cgggctggcg ttctggcgat acattctgac
     4141 taacaccacc gttcagtgcg tcacatcgtt cgccgcacgc aaattccgcc acgggcagat
     4201 ttactgcgta atgattggcc ttaagcgtgt aggaaccatc aagaaatact tcaaaggcgt
     4261 ggatgacgtg acgttttaca gcgccacacg cgaagaacta atcgacttcc tgaatcacgg
     4321 gagatagcca tgttatatgc atttaagctg ggcagaaaac tgcgcggcga ggaaccttgg
     4381 tgccctgaaa aaggcgggaa aggtggtagc tctgataaaa gcgcaaagta tgcagcagaa
     4441 gcccagaagt atgccgcaga cctgcaaaat cagcagttca acaccatcat gaacaacctg
     4501 aagccgttta ctcctctggc tgagaagtat gtcggcagcc tcgagaactt atcgtctctg
     4561 gagtggcaag gtcaggcgct taaccagtat tacaactctc agcagtacaa agaccttgct
     4621 ggtcaggcgc gctatcagag tctggcggca gcggaagcaa caggtggatt aggttccacc
     4681 gcaaccagta atcagttagc aacaatcgca ccaacgcttg gtcagcagtg gctgtctggc
     4741 cagatgaaca actaccagaa tctggcaaat attggtcttg gcgctcttca ggggcaggca
     4801 aacgccgggc aaacatatgc caacaacatg agccagattt cacagcaaag cgcggcactg
     4861 gcggcggcaa acgctaatag accttccggc cttcaatctg caataggcgg ggctgcctct
     4921 ggagcaattg ctggagcaca gcttggcagc attgttcccg gtattggtac tggtattggt
     4981 gccgctgttg gcggcggtct tggtcttctt ggttcactgt tttaagggtt aatcaatggc
     5041 tacgtggcaa cagggtatta attctggtgg ttttctggct ggcattggtg cgcaaaatga
     5101 gaatgcgcca aaagcaagcg acattaacgc aacgcttggt ctgatccgcg aaaacaatga
     5161 actggctcgc tcaggtgcaa ataacgttgg tctgaccgcg ttacgtggtc tggctggagt
     5221 tgctgatatt tataagcagc agcaacagca ggagcgtaaa gcggcattcc agaaaggtta
     5281 tgcagatgct tatgcgtccg gcgacaggga gcagatgcgt aatcttatta cagcattccc
     5341 tgaagagttt gaggaagtcc gtaaagggat ggggtatgtc gatgacgccc agcgggatga
     5401 ttttggcaat ctggcgctta aggctcaggt cgcttcgtcg cttggtccgg gtgcatttgg
     5461 caggtttatg atggatagcg aaaaggagat gcgtcgttta ggtatccctc cagaaactat
     5521 tgcggaaatg caggttaatg acccgcaggg cttccagcac ttcgcaggta atctggcact
     5581 gttttctctc ggccatgaga agtacttcga tatcaaagat cgaatggagg ggcgtcgact
     5641 tgaaggagaa agaaatcagt taaccgctcg tggtcaggat atcacgatgc gcggtcagga
     5701 tttatctgct gctactgctc ggagaggtca ggatttagcg atgcagagag catcaatgaa
     5761 aggggctgtt ggtaataatg agcgcacagt tcaactagca gacggaagaa ctgtaacggt
     5821 aggcgggaag cttcacggcg ctggggctaa tgcgttctac gaaggtatcg acaacgaggg
     5881 gaatatggtt cgcgttcctg ctggctctat tgccgctccg gctacatcgg cagcaagcgc
     5941 gcagaattac gcaatgaaga aagatcttga tgcaatttct ggtgcatcaa ttgacgatct
     6001 tggcttcatg actggcatta caggctcttc aggttctcct gctcttggtg cagatattcg
     6061 tagccgtgca tctggtggtg atcagaggaa actatacaac gctgcacagc gaatccaagg
     6121 aaagatgcag aatcagggca ttgcagcagc cagagacatg ggggcatccg gtatcaacac
     6181 cgttgcagaa gcaaagatgt attttcaagg tatgccacag gttgatttct caagccctga
     6241 agcactgcaa caatcaatgc gcgacattca gcaatatacc gacaattaca accaacaata
     6301 taacgttaat gttggtaaat ctcagcggca gcaatctcaa cctacacagg tatcacagcc
     6361 agcagccagc agtaactttt cttcactatg gggtgattaa tggctaaagc atggaaagat
     6421 gttatcgcct ctccacagta tcaggcgtta actgaagaac agaaagcaca ggctcaagcg
     6481 caatattttg atgaggttgt tgcccctaag gctggtgaca aatgggctga agcaagagat
     6541 cagttttatg cagcataccc tccgcctcag cagcagaaag aagaaccatc attgatgcaa
     6601 caagctggcg attggctcac tggtggtcaa agtgcagggc aaattgcaga acaggctggt
     6661 cgtggtctgg taaatatacc atttgacgta ttgcagggtg gcgcaagtct gattaatgca
     6721 atcagccagg ggcttggtgg ccccaaggtt ttggatgatg tttatcgccc tgtcgatcga
     6781 ccgacagacc cctacgctca agctggagaa acaattgg
//
LOCUS       DAZUFB010000178         6733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZUFB010000178 DAZUFB010000000
VERSION     DAZUFB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.178"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..879
                     /gene="exuR"
                     /locus_tag="ACHY3M_004728"
     CDS             103..879
                     /gene="exuR"
                     /locus_tag="ACHY3M_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFS4319487.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1119..1202
                     /gene="yqiM"
                     /locus_tag="ACHY3M_004729"
     CDS             1119..1202
                     /gene="yqiM"
                     /locus_tag="ACHY3M_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFS4319488.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1224..1886
                     /gene="yqjA"
                     /locus_tag="ACHY3M_004730"
     CDS             1224..1886
                     /gene="yqjA"
                     /locus_tag="ACHY3M_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFS4319489.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1890..2273
                     /gene="mzrA"
                     /locus_tag="ACHY3M_004731"
     CDS             1890..2273
                     /gene="mzrA"
                     /locus_tag="ACHY3M_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFS4319490.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2420..2788
                     /gene="yqjC"
                     /locus_tag="ACHY3M_004732"
     CDS             2420..2788
                     /gene="yqjC"
                     /locus_tag="ACHY3M_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFS4319491.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2826..3131
                     /gene="yqjD"
                     /locus_tag="ACHY3M_004733"
     CDS             2826..3131
                     /gene="yqjD"
                     /locus_tag="ACHY3M_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFS4319492.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3134..3538
                     /gene="yqjE"
                     /locus_tag="ACHY3M_004734"
     CDS             3134..3538
                     /gene="yqjE"
                     /locus_tag="ACHY3M_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4319493.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3528..3827
                     /gene="yqjK"
                     /locus_tag="ACHY3M_004735"
     CDS             3528..3827
                     /gene="yqjK"
                     /locus_tag="ACHY3M_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFS4319494.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4013..4405
                     /gene="yqjF"
                     /locus_tag="ACHY3M_004736"
     CDS             4013..4405
                     /gene="yqjF"
                     /locus_tag="ACHY3M_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS4319495.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4475..5461
                     /gene="yqjG"
                     /locus_tag="ACHY3M_004737"
     CDS             4475..5461
                     /gene="yqjG"
                     /locus_tag="ACHY3M_004737"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFS4319496.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5754..6119
                     /gene="yhaH"
                     /locus_tag="ACHY3M_004738"
     CDS             5754..6119
                     /gene="yhaH"
                     /locus_tag="ACHY3M_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4319497.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6361..6717
                     /locus_tag="ACHY3M_004739"
     CDS             6361..6717
                     /locus_tag="ACHY3M_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4319498.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT         1704 a         1627 c         1653 g         1749 t
ORIGIN      
        1 tcaatacatt aattcaatta ccaaatttat atatgaccaa tggcataccc atgaataaag
       61 tggtatgata acttcgtccg cattccactc cgggagacgc atatggaaat cactgaacca
      121 cgccgtttgt atcaacaact tgccgctgac ctgaaagagc gcatcgaaca gggcgtctat
      181 ctggtaggtg ataaactgcc tgcagaacgc tttattgccg atgaaaagaa cgtcagccgt
      241 acggtagttc gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa
      301 ggttcaggta ttcatgtggt ttccaaccag ccgcgccatc aacaggcggc tgacaataat
      361 atggaattcg ccaattacgg tccgtttgag ttgcttcagg ctcgccagct catcgaaagt
      421 aatattgccg agttcgcggc aactcaggta acgaaacaag acatcatgaa actgatggcc
      481 attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga tttacagttc
      541 cacattcagg tagccctggc gacgcagaac tccgccctgg cggccatcgt tgaaaaaatg
      601 tggacccaac gtagccataa cccgtactgg aaaaaactgc acgaacacat tgattcccgt
      661 accgtcgata actggtgtga tgaccacgat caaatcctca aggcgctgat tcgcaaagat
      721 cctcatgctg ctaagctggc aatgtggcag cacctggaaa acaccaagat catgttattc
      781 aacgaaacca gcgacgactt cgagttcaat gccgaccgct atctgttcgc cgaaaacccg
      841 gtagtgcatc tcgatacggc caccagcggc agtaaatgaa atacctgtcc gacaggcgct
      901 gcgacgcgcc tgtttgtctc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat
      961 cctctcgcca ccctcccctg cattcagcaa aatcagactc cacggacatg taattttgat
     1021 aacgaacaac gttgaccttt gttacaatta gattcaattt gaatttatgt ttttgaatgc
     1081 tttcttatct cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca
     1141 gaacatacca aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt
     1201 aaccgattca ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg
     1261 cgcaggattt tgaaaccctg gccaatccat cgatgattgg catgttgtat tttgtcttgt
     1321 ttgtaatttt gttccttgaa aacggcttgc ttccagcggc ctttttaccg ggcgacagtt
     1381 tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat gggttacccg caaacgattc
     1441 tgctgctgac cgttgccgcc agcctcggct gctgggtcag ctatattcag gggcgatggc
     1501 tgggcaatac ccgcaccgta caaaactggc tatctcattt acccgcgcat tatcatcaac
     1561 gcgcacacca tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg
     1621 cgtttgtcag aacactgctg ccgacgattg ccgggttatc agggctgaat aacgcgcgct
     1681 ttcagttttt caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt
     1741 acatgctcgg caaaacgccg gtatttttaa agtacgagga ccagctgatg tcatgcctga
     1801 tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtgttatgga
     1861 aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc
     1921 tagcctggtc cggcactgtt ttacttctgg tcggcacgct gttgctggcc tggtccgcgg
     1981 ttcgccagca agagtctacg ctggcgattc gtgccgttca tcaaggcaca acgatgccag
     2041 acggtttttc gatctggcat caccttgacg ctcatggcat tcctttcaaa agtatcaccc
     2101 ccaaaaacga cactctgtta attacatttg actccagcga ccagagcgcc gccgcaaaag
     2161 cggtcctcga cagaacattg ccccatggct acatcattgc gcagcaggac aataacagtc
     2221 aggctatgca gtggctgacc cggttacggg ataactctca tcgcttcgga taacttcctg
     2281 ttatccgaaa catatcactc actttggtga tttcaccgta actgtctatg attaatgagg
     2341 cggtggttaa cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat
     2401 cacaatggaa ggttcaagaa tgaaataccg catcgcttta gctgtttctc tctttgctct
     2461 tagtgccggt agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga
     2521 gatcagctat gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct
     2581 gagtgaagtc cgggccaact gttcagatag ccagctgcgt gccgatcatc acaagaaaat
     2641 cgcaaagcag aaagatgagg tggcggaacg ccagcaagat ttagccgagg cgaagcaaaa
     2701 aggcgatgcc gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct
     2761 gaaaaagctg gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga
     2821 aaactatgtc gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg
     2881 atacgctgga agaggtgctt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga
     2941 ttcgtagcaa agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg
     3001 atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc tgatgagtat gtgcgtgaaa
     3061 atccgtggac cggcgtgggc attggcgctg caatcggtgt agtgctcggc gttctgctgt
     3121 cgcgtcgtta attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg
     3181 catcgggcag cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc
     3241 ggtggtggag ctggaagagg aaaaagctaa tctctttcaa cttttactga tgctgggcct
     3301 gacgatgctt ttcgctgcat ttggtcttat gagcctgatg gtgctaatta tttgggcggt
     3361 tgatccgcaa tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc
     3421 actaattggc ggtatctgga cgttacgtaa atcgcgtaaa tccaccttgc tgcgccatac
     3481 acgccatgag ctggcaaacg atcggcaact gctcgaggag gagtcccgtg agcagtaaag
     3541 tcgaacgtga acgacgtaag gcgcaactgc ttagccagat ccagcaacaa cggctggatc
     3601 tttccgccag tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata
     3661 tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag cgtgatggcg atctggacga
     3721 ttcgccatcc taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct
     3781 ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg cggttaactt ccctctggcc
     3841 ggagcaattc cggccttatc cctcaaattt cttgaagatt tttgacagtt ttccttgcta
     3901 acaatcatca ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc
     3961 gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa
     4021 attagaagat gttggtgtac tggtagcgcg cattttaatg ccgattctgt ttattaccgc
     4081 aggctgggga aaaatcaccg cttactctgg tacccaacaa tatatggaag caatgggtgt
     4141 cccgggtttt atgctgccgc tggtgattct gcttgagttc ggtggtggtc tggcaatcct
     4201 gttcggtttc ctgactcgca ccacagccct gtttactgcg ggctttacgc tgctgacggc
     4261 atttttattt cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct
     4321 gacaatttct ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga
     4381 ccgcctgctg aataaaaagt ggtaagcacg cgctatactt aacgataaaa aacgaggagg
     4441 aagctcctcg tttttgctat tggaggagag aaaaatgggg caactgattg acggcgtctg
     4501 gcatgacacc tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc
     4561 atttcgtaac tggctcactg ccgatggcgc acctggcccc actggcacag gcggttttat
     4621 cgcagagaaa gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac
     4681 gctgatcatg cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc
     4741 gctgatgctg gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagatac
     4801 gctctatcaa catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg
     4861 acgagttact gttcccgtgc tgtgggacaa aaagaaccac accatcgtca gcaacgaatc
     4921 agcagaaatc atccgcatgt tcaataccgc gtttgatgcg ctgggcgcaa aagcgggtga
     4981 ttactaccca ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac
     5041 cgttaacaac ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaggc
     5101 ggtggcgaaa gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta
     5161 cctgaccggc aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtccgttt
     5221 tgatccagtg tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attaccttaa
     5281 tctgtatggc ttcctgcgtg atatctacca gatgccggga attgccgaaa cagtcaattt
     5341 cgatcacatc cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat
     5401 ttcaattggt ccatggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta
     5461 aaaaaaggca ccttacgagg tgcctctgat aattagctaa gtgcatgttg ttcatgtcgg
     5521 atgcggcgtg aacgccttat caaacctaca aaacatttat attcaatatg ttgcaatgac
     5581 cgcgcaggcc agataagcgt agcgcatctg gcgattttgc gcttgtcatc agtcttagcg
     5641 cgccttcaca gcactttatt aattccttga acgaatattt actgccattt cattcacgtc
     5701 tattcttaat ttgctgcttt aaaaacaagt gattgagcaa aattgaggca aaaatggact
     5761 ggtatctgaa agtactaaaa aattatgtcg gtttccgggg tcgtgcgcgg cgcaaagagt
     5821 actggatgtt tattctggtc aacatcatct ttacgttcgt gctggggctg ctggataaaa
     5881 tgttaggctg gcaacgcgct ggtggcgaag gcatcctgac gacaatctac ggtattctgg
     5941 tgtttttacc gtggtgggca gttcagttcc gccgcctgca cgacaccgac cgctcggcgt
     6001 ggtgggcact gttgttctta atcccgttta tcggctggct tattatcatc gtctttaact
     6061 gccaggcggg tacgccaggc gaaaaccgct ttggaccaga tccgaaactg gaaccttaaa
     6121 acaaatctgc tcgccggatg tctgactcat ccggctttta tcatcatgtt gatgaatgaa
     6181 taatttacta atgtttattt aaaatatttc aatgtcgtta tataaattac ctataaaaaa
     6241 caaccatgta tttatcttaa atatcaaaac ctttataaca tccgaaaaag aaagatacga
     6301 atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag ggatgttttt
     6361 atgcagtggt atttagccgt attaaaaaat tatgttggtt tctctggtcg cgcacgtcgt
     6421 aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat caatgtcatt
     6481 caattgattt taggtttaga gtttccattc ctttctctca tttacctggc agcaacaatc
     6541 attccggtca ttgctctgtg tgttcgtcgt ctgcacgata ctgaccgttc aggtgcgtgg
     6601 gcgcttctct atctggtacc gatcattggc tggttagttc tgtttgtatt tgcctgttta
     6661 gaaggcaatt ctggcagcaa tcgttacgga aacgatccta agtttggttc aaattaatct
     6721 tagaattggg gcg
//
LOCUS       DAZUFB010000179         6723 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZUFB010000179 DAZUFB010000000
VERSION     DAZUFB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.179"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..144
                     /locus_tag="ACHY3M_004740"
     CDS             <1..144
                     /locus_tag="ACHY3M_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFS4319499.1"
                     /translation="KKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
     gene            851..1039
                     /locus_tag="ACHY3M_004741"
     CDS             851..1039
                     /locus_tag="ACHY3M_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319500.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAK"
     gene            1214..1537
                     /locus_tag="ACHY3M_004742"
     CDS             1214..1537
                     /locus_tag="ACHY3M_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4319501.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPS
                     ISPVLLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKG
                     ADDDR"
     gene            1746..2180
                     /locus_tag="ACHY3M_004743"
                     /pseudo
     CDS             1746..2180
                     /locus_tag="ACHY3M_004743"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2215..2712
                     /locus_tag="ACHY3M_004744"
     CDS             2215..2712
                     /locus_tag="ACHY3M_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFS4319502.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSES
                     EKRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQSQQ
                     ILDNYTTIREQKDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGRKNGI
                     RLVQE"
     gene            2719..2934
                     /locus_tag="ACHY3M_004745"
     CDS             2719..2934
                     /locus_tag="ACHY3M_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFS4319503.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQREN
                     AALSERVTGLSRQVQSLSEQLRRLSQG"
     gene            complement(3073..3366)
                     /locus_tag="ACHY3M_004746"
     CDS             complement(3073..3366)
                     /locus_tag="ACHY3M_004746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319504.1"
                     /translation="MAINFTDVVNQLTSDAFATLTAARNGLAASRDLYHFQMAIHEFG
                     EERVVQECASVLKERYRCSYAEAAAEAAHRVKGFLELSAGENTFRQVRENLQR"
     gene            complement(3789..4034)
                     /locus_tag="ACHY3M_004747"
     CDS             complement(3789..4034)
                     /locus_tag="ACHY3M_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319505.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            4298..6391
                     /gene="cda"
                     /locus_tag="ACHY3M_004748"
     CDS             4298..6391
                     /gene="cda"
                     /locus_tag="ACHY3M_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFS4319506.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            6388..6651
                     /locus_tag="ACHY3M_004749"
     CDS             6388..6651
                     /locus_tag="ACHY3M_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFS4319507.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
BASE COUNT         1658 a         1539 c         1911 g         1615 t
ORIGIN      
        1 aaaaaaaaga tagggggatc tatgattctc gttcttgctg ttttgtgtct gactgcttgc
       61 caagctaact atgtaaggga tgttcagggt ggaacaattg ctccatcatc atcttcgaaa
      121 ctaatcgggg tggcggttca gtgaaaaata ttaaaggatc ttcttgagat cctttttttc
      181 tgcgcgtaat ctgctgcctg taaacgaaaa aacccgcctt gtggcgggtc cttccgtccg
      241 atcagaagtc ttcgacctct tctaatcggc ggtggtaact ggcttggagg agcacagtca
      301 ccaaaactgt cttttcagtt tagccttaac tggttcataa cttcaagagc aactcctcta
      361 aatcagttac caatggctgc tgccagtggc gttaagtcgt gtcttttccg ggttggcccc
      421 aagagaacag ttaccggaga aggtgcagca gtcgggctga acggggggtt cctgcataca
      481 gcccagcccg gagcgaactg cctaagtcgc cccgagatac cagaagacgt gagctatgag
      541 aaagcgccac gtgtcccgca agggagaaag gcggaaaagg tatccggtaa tcgatatggc
      601 gggaacgtgg gggcgcagga gggagcgatt caccggaaac ggtgatgatc tttaagccac
      661 gtcgggtttc gccaccgctg gcggatttca tggttgagcc tcacggctcc cacagatgca
      721 ccgaaaaagt gtctgttttg tgaagtccgt caggggggcg gagcctatgg aaaaagcgcc
      781 tccggcgcgg cctttttcgg ggttttcgtt tcttgctcac atgttctttc cggcgttatc
      841 ccctgattct gtggataaca ctatcaccgc ttttgagtgg gcagataccg ctcgccgcag
      901 ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgagca
      961 ttttctcctt acgcatctgt gcggcatttc acacccggca tggtgcactt ttcatacaat
     1021 cctcactgat gccaaatgat taagccagta tacactccgc tagcgctacg tgactggttc
     1081 agggctgcgc cccgaaaccc gctaaaagcc actgacgcgc ctgcggcttg tccaacaccg
     1141 cgccgaccgg gaaagaattt cccgcccgtc ccggcgttat caggaggccg gattggcaga
     1201 caaacgcagc aaaatgctca cgatgtgggt aacggaggac gaacaccgcc gtcttctgga
     1261 acgctgcgac ggtaagcagc tcgcagcctg gatgcggcag acgtgtctgg acgagaaacc
     1321 ggctcgggct ggcaaacttc cgtcgatctc gccggtgctg cttcgtcagc ttgccggcat
     1381 gggtaacaac ctcaaccaga ttgcccggca ggttaacgcc ggtggtggca gcgggcacga
     1441 ccgtgtgcag gttgtcgccg cgctgatggc cattgatgcc ggactcgaac ggctgcggca
     1501 tgccgtgctg gaaaaggggg cagacgatga tcgttaagtt tcacccgcga gggcgcggcg
     1561 gtggcgccgg tccggtggat tacctgctgg ggaaagaccg gcagcgcgac ggcgcaaccg
     1621 tcctgcaggg aaagccggaa gaagtccggg agcttatcga cgcctcgccc tacgccaaaa
     1681 agtacacctc cggcgtcctg tcttttgccg aacaggactt accgcccggc cagcgtgaaa
     1741 agctgatggc gagtttcgag aaggttctga tgcccggact cgacaaagac cagtacagcg
     1801 tgctgtgggt tgagcaccag gacaaggggc ggctggagct gaacttcctc atcccgaaca
     1861 cggagctgct gaccggcaaa cggctccagc cgtattacga ccgcgccgac cgtccgcgca
     1921 tagacgcctg gcagaccatc gtgaacggca ggctggggct gcacgacccg aacgcgccgg
     1981 agaaccggcg tgctctggtc acgccgtccg cgctgccgga agcgaagcag gaagccgccc
     2041 gggcgattac gcgggggtta cttgccttag cctcgtccgg ggagctgaaa acccgtcagg
     2101 acgtcactga ggcgctggaa agcgctggtt ttgaggtggt gcgcaccacg aaaagcagca
     2161 tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc atttatgaac
     2221 agtcttttaa cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagagtacc
     2281 ggcgagatgc tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc
     2341 gaaagcgaga agagaatcag cgccgccatc ctcgaccacg accggaagct gtcctcagcc
     2401 atgagccagc gcacgaaagg gatgctgcgc atggtcagcc agacgtggct gaccatcgtc
     2461 ctggtgtccg cgctgctgat cgcctcgagc gctggcattc tgtggtggca gagtcagcag
     2521 atactcgaca attacacgac catccgggag cagaaggatg cgctggagaa gctgaacgcg
     2581 cggacgtggg gcgtgaggta tcaggaagac aaccaggggc gttttctggt gctgccggaa
     2641 ggtgtgaaag cggacacgaa ctggacgttc gacaacggca ggaagaacgg catcagactg
     2701 gtgcaggagt agagcagcat gacggagctg gaaaaacagt tgctgagcgc attagagcag
     2761 ctacagcagg actactcgaa aaggctggac gagtgggaga acgccttcgc ggaatggcgg
     2821 acgatgtctg gtcttatgca acgggagaac gcggcgctga gcgagcgcgt cacgggcttg
     2881 agcaggcagg tgcagagttt gagcgagcag ctgcgccggt tgtcgcaagg ctgaatgata
     2941 tcgaggcaca ccgggagcag gagtacgcag cgcaacacca gaaagcgctg gagcaggagc
     3001 gaccgcagcg acagagaacg tacgatggcc ctactcttta ataacaaaag ccgcccaaat
     3061 ggacggcttt tgttatctct gtaggttttc tcgtacctgg cgaaatgtat tttctccagc
     3121 actaagttca aggaaccctt taactctgtg ggctgcttca gctgctgctt cggcataaga
     3181 gcagcggtaa cgttctttta gtacgctggc gcactcctga acaactcttt cttccccaaa
     3241 ttcgtgaata gccatttgaa agtgatagag atcacgagat gctgccaaac catttcttgc
     3301 tgcagttaaa gttgcaaaag catcagatgt taattggtta acaacgtcag tgaagttaat
     3361 agccatttta ctctccagat cgctttagtt cttttttgga agactagtgt actgcttagt
     3421 caaaatgaac gctgctaatt aataaccaaa atgttgtcat taccgccttt aagtggcgca
     3481 cagtggcgca tgaggactgt gtcccgacac cctgccaacg cctcacgggc gcacagtaaa
     3541 ctgcgcaccc gttcaccgtt atccggtgag tttggtgccc gaaacccccc tttgatggca
     3601 gcattcccgc gaaactgccc gccattcact cccgtgcata gccatgcagc cgcgcagaaa
     3661 tcatcagggg cgtttttcag ggttatggac agggaaaggt gccggaaacg acccgctcgt
     3721 ctcagaatgg cgctgagggc ccctgcgggg gtgcgggcaa taaggggtta tgttaaataa
     3781 cccttagctt agcggcggcg cagtggttgc caggtgaagg tgtcatccgg gtcctgtccg
     3841 gcagtcagtg tggcttctcg atttttgctg gccgttcgtt catcaagaat ggctttaagt
     3901 atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt tgtccttttt cgtcagcccc
     3961 atggcacgag cttcagcggc aatggcttct gccagtcggc gctcttcggc ttcggtcagt
     4021 ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg tcataacagt ttaactgttg
     4081 gctctctcct tatgctaatc agccttcttt catgcctgtt gccattgagt ttaagaatga
     4141 ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc cagtgggttg acaggattta
     4201 cgaggtgacg taacttttta ctgtacataa aaccagtggt tttatgtaca gtttgttgtt
     4261 tttaacgcat tgttttaaaa gtcaaagagg tgtttttatg agtgattacg aaggtagtgg
     4321 tccgacagaa ggtattgatt atgggcactc gatggtcgtg tggccgtcaa caggactgat
     4381 ttcgggtggt gatgtgaaac caggaggctc atcaggtatc gctccatcca tgcctccggg
     4441 atggggggat tacagcccac agggtatcgc acttgtacaa agtgttcttt ttccgggaat
     4501 tattcgccgg attattctgg ataaggaact tgaagaggga gactggtcgg gatggtctgt
     4561 cagtgtgcat agcccttggg gaaacgagaa agtttccgct gcacgaacag ttcttgagaa
     4621 tggtttacgt ggtggtttgc cagaaccgtc tcgcccggct gctgtttctt ttgcccgtct
     4681 ggagcctgct tccggaaatg agcaaaaaat tattcgtctt atggttacac agcaactgga
     4741 acaggtaacg gatattccgg tcagccagtt accagcagcg ggtaataatg taccggtgaa
     4801 atatcgtctg atggacctta tgcagaacgg tacgcaatat atggctatta tcggaggtat
     4861 tccgatgaca gtgccggtag ttgatgccgt tccagttccg gaccggagtc gtccgggaac
     4921 aaatattaaa gatgtttaca gtgcccctgt atcaccaaat ctaccggacc tggtattaag
     4981 tgtgggtcag atgaatactc cggttctgtc taatcccgaa attcaggaag aaggagttat
     5041 tgctgagaca ggtaattatg ttgaggctgg ttatacgatg tccagtaata atcatgatgt
     5101 cattgttcgt tttcctgaag gcagtggtgt ttctccgtta tatatttcaa ctgtagagat
     5161 tctggacagt aatggtctga gtcagcgtca ggaagccgaa aataaagcaa aggatgattt
     5221 cagagtcaag aaagaagaag cggtagcccg ggctgaagct gaaaaagcaa aagccgagtt
     5281 atttagtaaa gcaggtgtga accagcctcc tgtatataca caggaaatga tggaaagggc
     5341 taattcagta atgaatgagc agggagctct tgttctgaat aacactgcca gttctgtaca
     5401 actggcgatg accggaactg gtgtctggac tgctgctggt gatattgcag gcaacataag
     5461 caagtttttc agtaatgctc tggagaaagt aaccataccc gaagtgagtc ccctgcttat
     5521 gcggatttct cttggtgctc tgtggtttca ttcagaagaa gctggagcag gaagtgatat
     5581 cgtgccgggg cgaaatctgg aggcgatgtt ttcgctgagt gctcagatgt tggctggaca
     5641 gggcgtggtc attgaacctg gtgcgacgag tgtaaatctg cctgttcgtg gacaattgat
     5701 aaacagtaac gggcaattag ctctggattt actgaaaaca gggaatgaaa gtatcccagc
     5761 tgcggttcct gttcttaatg ctgtccgtga tacagcaaca ggactggata aaatcacgtt
     5821 accagcagta gtaggcgcac cttcccggac gattctggtt aatccggtac cacaaccttc
     5881 ggtgccaaca gatacaggta atcatcaacc cgttccggtt acaccagtgc atacaggaac
     5941 ggaagtaaaa ccggtcgaaa tgccagtaac gacgattact cctgtttctg atgttggtgg
     6001 actacgagat tttatttact ggcgtcctga tgctgctggg actggtgttg aagctgttta
     6061 tgtgatgctt aatgatcctc tggattcagg gcgattttcg cgtaaacaac ttgataaaaa
     6121 atataaacat gctggtgatt ttggtattag tgatacaaaa aagaatcgtg aaactcttac
     6181 taaattcagg gatgctattg aggagcattt atcggataag gatacagtag agaaaggaac
     6241 ataccgaaga gaaaaaggtt caaaagttta ttttaatcct aatacgatga atgtggttat
     6301 aattaagtca aatggtgagt tcttatctgg gtggaaaata aatccagatg cggataatgg
     6361 tcgaatttat ttagagacag gtgaactatg aacaagatgg caatgatcga tttggcgaaa
     6421 ttatttttag cttcgaaaat tacagcaatt gagttttcag agcgaatttg tgttgaacgg
     6481 agaagattgt atggtgttaa ggatttgtct ccgaatatat taaattgtgg ggaagagttg
     6541 tttatggctg ctgagcgatt tgagcctgat gcagataggg ctgattatga aattgatgat
     6601 aatggactta aggttgaggt ccgatctatc ttggaaaaat ttaaattata atcatgagtg
     6661 attttaatat ctggcattta agtataaatg tcagatattc actcatgaca aggagatgtc
     6721 atg
//
LOCUS       DAZUFB010000180         6683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZUFB010000180 DAZUFB010000000
VERSION     DAZUFB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.180"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1218
                     /locus_tag="ACHY3M_004750"
     CDS             <1..1218
                     /locus_tag="ACHY3M_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS4319508.1"
                     /translation="NNMTVNDDANDVAVTESYKPIWNWKISQYNVFDIKFETMIKPQF
                     ADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNI
                     EDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQ
                     TGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTY
                     AGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAII
                     NKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNG
                     NGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNG
                     LMPLIRRRTMLQL"
     gene            1408..1521
                     /gene="yhiY"
                     /locus_tag="ACHY3M_004751"
     CDS             1408..1521
                     /gene="yhiY"
                     /locus_tag="ACHY3M_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFS4319509.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1584..2651
                     /gene="yhiI"
                     /locus_tag="ACHY3M_004752"
     CDS             1584..2651
                     /gene="yhiI"
                     /locus_tag="ACHY3M_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4319510.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2648..5383
                     /gene="rbbA"
                     /locus_tag="ACHY3M_004753"
     CDS             2648..5383
                     /gene="rbbA"
                     /locus_tag="ACHY3M_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFS4319511.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFSVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5383..6507
                     /gene="yhhJ"
                     /locus_tag="ACHY3M_004754"
     CDS             5383..6507
                     /gene="yhhJ"
                     /locus_tag="ACHY3M_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4319512.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1578 a         1654 c         1852 g         1599 t
ORIGIN      
        1 aataatatga cagtaaatga tgatgctaat gatgttgcag ttactgagtc gtacaaaccc
       61 atctggaact ggaaaatatc gcaatacaat gtatttgata ttaaattcga aacaatgata
      121 aaaccccagt tcgctgacag aacctatttc tccaattgtt cacctgttga tcccacaagt
      181 accagaccaa cctattttgg cgataccgat ggctcagtcg gtgcagtttt gtacgcgctt
      241 tttgcaacgg gacatttggg aatcatggct gaaggagaaa atttccttag ccagttgctt
      301 aatatagaag atgaagttct caacgtattg ctaagggaga actttaatga gcaacttgac
      361 actaatgtta atataataat cagtatcctg aacagaagag acattgtttt agaaagtctt
      421 cagccttatt tggttattaa taaagacgca gtcacgccat gtacgtttct gggcgaccag
      481 actggcgatc gatttagtaa tatctgtggc gataaattta tcatcgattt attaaaacgc
      541 ataatgagta tcaatgaaaa cgttcatgta ttagctggaa atcatgaaac gaattgcaat
      601 ggtaactata tgcagaactt cacgcgcatg aaaccacttg atgaggatac ctacgctggg
      661 ataaaagatt atcctgtatg tttctatggc cccaaataca aaataatggc aaatcatcat
      721 gggataactt ttgatgatca gcgaaaacgt tacattatag gaccgataac agtctctagt
      781 gatgaaatga ccaatgctct cgatccggtc gaattagcgg caattatcaa taaaaagcac
      841 catgctgtaa ttaatggcaa aaagtttaaa aatagccggg ctatctcttg tcgatccttt
      901 aatcgttatt tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgc
      961 tcacaaatgt taggtataaa tcaagttgtt gcacataacg gtaatggtgg aagagaatgt
     1021 ataggtgaaa cggggacagt tttaggtctt aacgcccgtg acagtaaaca tgctggaaga
     1081 atgttcagca tgcataattg ccaaattaac cctggtgcag ggcctgagat aaccactccc
     1141 tggaagtctt accagcatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc
     1201 acaatgttac agctctgaac catagacaac atgaatatta atgttactat ctggttgaga
     1261 ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgcg
     1321 atccaacagg atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc
     1381 gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag
     1441 ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc
     1501 ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt
     1561 acagggatta tggcgggccg attatggata agagtaagcg ccatctggcg tggtgggttg
     1621 tcggggcact ggcggtggct gccgtcgtgg cgtggtggct gttgcgcccg gcgggtgtgc
     1681 cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca
     1741 aaattgccgg gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggtg
     1801 aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc
     1861 aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa
     1921 gcgaaactcg cgccgcgcag tcgctggtta atcaacgcca ggcagaactg gactccgtag
     1981 caaaacgtca tatgcgttcc cgttcactgg cccaacgagg ggctatttct gcgcaacagc
     2041 tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg gcaaaagctc
     2101 aggtttctgc ttctaaagcc gctatagagg cggcacgtac caatatcatt caggcgcaaa
     2161 cccgcgtcga agcggcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg
     2221 aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc
     2281 tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct
     2341 tcctgccaac cgaacaggcg ggcacgctga aactgggtgg tgaagcccgg ctgatcctcg
     2401 atgccgcgcc agatctgcgt attccggcga ccatcagttt tgtcgccagt gtcgcccagt
     2461 tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag
     2521 cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc ggtttaccgg
     2581 gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt
     2641 tgccgcaatg acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt
     2701 gagccagcat tatggaaaaa ccgttgcgct gaacaatatt actctcgata ttccggcccg
     2761 ctgtatggtc gggctgattg gtccggatgg cgtcgggaag tcgagcttat tgtcgttgat
     2821 ttccggtgcc cgcgtcattg agcagggcaa tgtgatggtg ctgggcggcg atatgcgcga
     2881 cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa
     2941 aaatctctac cacaccttgt cggtgtatga aaacgtcgat tttttcgctc gcctgttcgg
     3001 tcacgacaaa gcggagcggg aagtgcgaat taatgagctg ctgaccagca ccgggttagc
     3061 accgtttcgc gatcgtccgg cagggaaact ctccggcggg atgaagcaaa aactggggct
     3121 gtgctgtgcg ttaatccacg acccggaact gttgattctt gatgagccaa caacgggggt
     3181 tgacccgctc tcccgcgccc agttctggga tctgatcgac agtattcgcc agcggcagag
     3241 caatatgagc gtgctggttg ccaccgccta tatggaagag gccgaacgct tcgactggtt
     3301 ggtggcgatg aatgccggag aagtgctggc aaccggcagc gccgaagagt tgcggcagca
     3361 aacgcaaagc gccacgctgg aagaagcatt tataaatctg ttaccgcaag cacaacgcca
     3421 ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aacgcagaga ttgccatcga
     3481 agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg tcaatttccg
     3541 cattccacgc ggggagattt ttggattcct tggttcgaac ggctgcggta aatccaccac
     3601 catgaaaatg cttaccggac tgctgcccgc cagcgaaggt gaggcgtggc tgttcgggca
     3661 accggttgat ccaaaagata tcgatacccg ccgtcgggta ggctatatgt cgcaggcgtt
     3721 ttcgctctat aacgaactca ccgtgcggca aaaccttgag ttacatgccc gtttgtttca
     3781 catcccggaa gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt ttaagctcaa
     3841 cgacgttgaa gatgttctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct
     3901 ggcggtggcg gtgattcatc gcccggagat gttaatcctc gatgaaccta cttctggcgt
     3961 cgatccggtg gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa
     4021 agtgactatc ttcatctcca cccactttat gaacgaagcg gaacgttgcg accgcatctc
     4081 actgatgcac gccggaaaag tgcttgccag cggtacaccg caggaactgg ttgagaaacg
     4141 cggagccgcc agtctggaag aggcatttat cgcctatttg caggaagcgg cagggcagag
     4201 caacgaagcc gaagcgccgc ccgtggtaca cgacatcacc cacgcgccgc gtcagggatt
     4261 tagcctgcgc cgtctgttta gctacagccg ccgcgaagca ctggaactgc gccgcgatcc
     4321 ggtccgttcg acgctggcgc tgatgggaac ggtgatcctg atgctgataa tgggttacgg
     4381 catcagtatg gatgtggaaa acctgcgctt ttcggtgctc gaccgcgacc agaccgtcag
     4441 tagccaggcg tggacgctca atctctccgg ttcccgttac tttatcgaac agccgccgct
     4501 caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg tggcaattga
     4561 gatccctccc aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg
     4621 gattgacgga gcgatgccca gtcgtgccga aacggtaaaa ggttacgtgc aggcaatgca
     4681 ccagagctgg ttacaggatg tggcgagccg acaatccaca cccgccagcc aaagcgggct
     4741 gatgaacatt gagacgcgtt atcgctataa cccggacgta aaaagcctgc cagcgattgt
     4801 tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt
     4861 ggtgcgggaa aaagagcttg ggtcgattat caacctttac gtgaccccca ccacgcgtag
     4921 tgaatttttg cttggtaaac agttgccata catcgcgctg gggatgctga actttttcct
     4981 gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct
     5041 caccctggcg gcgctgctgt atatcatcat tgccaccgga atggggctgc tgatctccac
     5101 ctttatgaaa agccagattg ccgccatttt cggaacggcg attatcacgt tgatcccggc
     5161 gacacagttt tccgggatga tcgatccggt agcttcgctg gaagggcctg gacgttggat
     5221 cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcgggacgt tctcgaaagc
     5281 gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca tcccgctggt
     5341 gatgggctta agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat
     5401 atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc gatgctgacg
     5461 ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg
     5521 ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc
     5581 gttaacagct tctatcgtcc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg
     5641 gatgccggac tggacgccgg acgctatacc ttcgcgataa atattccgcc taattttcag
     5701 cgtgatgtcc tcgccggacg ccagccagat attcaggtga acgtcgatgc cacgcgcatg
     5761 agccaagcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc
     5821 tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc
     5881 tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcaatcat caacaacatt
     5941 accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg
     6001 gtggaacact tgttggtgat gccgataacg ccgtttgaga tcatgatggc aaaggtctgg
     6061 tcgatggggc tggtggtgct ggtggtgtcg ggattatcac tggtgctgat ggtgaaaggc
     6121 gtgctgggtg taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt
     6181 ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa
     6241 ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggatccacg
     6301 ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac
     6361 tttgttagcc tcgcgcaggc catcctctac cggggtgccg gattcgaaat cgtctggccg
     6421 cagttcctga cgctgatggc aattggcggc gcgtttttca ccattgcact gctgcgattc
     6481 aggaagacga ttgggacaat ggcgtaaata caattgcccg atgcgtaagc ttatagggac
     6541 tacgcatcgg tgcaatttat tgaattttag ttgtttttag gccggataag gcgtttatga
     6601 actgaccccc aaaacctgga cagtttcagt taaccggctt tttgatactc agtcctgtat
     6661 tcagggcaag attacgaaag ccc
//
LOCUS       DAZUFB010000181         6475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZUFB010000181 DAZUFB010000000
VERSION     DAZUFB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.181"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..241)
                     /locus_tag="ACHY3M_004755"
     CDS             complement(<1..241)
                     /locus_tag="ACHY3M_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319513.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQ"
     gene            complement(648..1775)
                     /locus_tag="ACHY3M_004756"
     CDS             complement(648..1775)
                     /locus_tag="ACHY3M_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4319514.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLDTSFCHIFDFYTEENLTNYL"
     gene            complement(1858..2346)
                     /locus_tag="ACHY3M_004757"
     CDS             complement(1858..2346)
                     /locus_tag="ACHY3M_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFS4319515.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(2437..3393)
                     /locus_tag="ACHY3M_004758"
     CDS             complement(2437..3393)
                     /locus_tag="ACHY3M_004758"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFS4319516.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAQNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(3412..4449)
                     /locus_tag="ACHY3M_004759"
     CDS             complement(3412..4449)
                     /locus_tag="ACHY3M_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS4319517.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            4722..5954
                     /locus_tag="ACHY3M_004760"
     CDS             4722..5954
                     /locus_tag="ACHY3M_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4319518.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            5957..>6475
                     /locus_tag="ACHY3M_004761"
     CDS             5957..>6475
                     /locus_tag="ACHY3M_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4319519.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVD"
BASE COUNT         1937 a         1275 c         1193 g         2070 t
ORIGIN      
        1 tttgtgaaag tatgagttca ggagattttg cttcatattc tttagtttca ccatcgacaa
       61 gaaagatata accgttcttg taaagattat caatagatga gtatacagac gaacgagaaa
      121 gtgaaatttc ttttgctatc ttgtaaccac tgctacgacc gtttttcaac agactgatgt
      181 aaacctgtgc atcggtgcga gtgaaaccaa aatttatcaa tttatttacc aaatgtatca
      241 tagacgcgct cctgaaatga aaatgaaatc ttaccaattt taacattatt aattaacatt
      301 ttcgaaaaaa ttgctgagct aaaatgtgga tttatcgtaa ttcatcctca agctggttac
      361 aggtctacag tacgtaagca ttgtgaactg cgaatctgaa tgccctccaa ctatggtcag
      421 aagacatgct ctgacctaaa tagtaggctc actgagtaag cggtgttatc aacggtttat
      481 tcatatatgc tcttttttat ggatggaatt ttgtaactca attaccttga taagaatcag
      541 ttcgtggagt taatgacatt taccaataga cagttcgagc agtaagaagc cttatggaat
      601 tgtaaggttt aataccgtta ttcaacctgg ggttattgtc atatcaatca caaataatta
      661 gttagatttt cttcagtgta aaaatcaaat atatgacaga agcttgtatc aaggttgaaa
      721 tacaaatttt taccaatatc ggctgtagta attgattgat cgttgacttt tgcaataaac
      781 tctttgttgt taacggtaaa gtaaagatat ttttcactgc ccatattttc aataatactt
      841 aatgcaccag tgactgtatt catttctttg caattcaaac tcaaagagat atgttctgga
      901 cgaataccaa aacaaacggg tttatctata tacgctttga gtactgattg tttattctca
      961 ggtatgtaca accgaatatt tttggcaatg tcaatataga gtttaccgtc ttcctcacgt
     1021 acaaccgcat ctatcaaatt cattgacggg gaaccaataa agccagcaac gaatctgtta
     1081 gcagggtggt tatacagatt aactggtgtg tcgacctgca ttattcgccc ctgattaagc
     1141 acacaaatac gatcaccgag ggtcaatgct tcaatttgat cgtgtgtaac ataaatcatg
     1201 gttgctggat gaccctcatc cttgagagac tgatgcaact gagcaatttt gactcgcatt
     1261 gaaacacgaa gttttgcatc aaggttagat agtggctcat caaataagaa aacatctggc
     1321 ttacggacaa ttgcacggcc taccgcgaca cgctgacgct gaccacccga catttctttc
     1381 ggttttcggt aaagtaaatc gctaatttca agtttttctg caacttcttc gacaattttc
     1441 ttaatctctt cttttggcct tttttgcatt tttaaaccaa aagccatatt gtcaaataca
     1501 gtcttatggg gatacagcgc atagttctgg aacaccattg caatgctgcg atctttaggc
     1561 ggaagggtat taacaaggcg gtcgctaatg taaatttcac cacctgatac gtcttcaaga
     1621 cctgcaatca tgcgtaatgt agttgatttg gcacacccag atggtccaac aaaaaccatg
     1681 aattcaccat cgtttatatc gagatcaatg ccgtgaacag ctttgaatcc attgtcataa
     1741 attttttcaa ctttgcgtag aattaccgcg gccataatct gctctcatta acaagaattt
     1801 tggaatactg atactccact attcaacgaa tgttgaatag tggagtgcta aaaataacta
     1861 tttacatggt gctaccatgg cggctattgc ctttttatca ggaccaattt gaatcgtcca
     1921 aacatacttt ccatcggcag gaataacgac ttttgtattt ttatccatgc ctcctttctt
     1981 gagtgtttta gcctgattta atggcaattc aagacctgtt acagtgaact gcgggttcca
     2041 gttcatagtc gcaaattgca ttgttaccgc acctgctttt tcagaataaa taagctgata
     2101 aataccatct cccttataat gcattttacg ctctggcttc attaaccaat agccttccgg
     2161 gagggttccg gaaatgtaaa gacccgcagc aattggccct ctttcattta catcagggag
     2221 gttacattct gaatagcgag gatccgcttt cacgacacct tcctgactgc cagagggtga
     2281 attgccatca acgcatcctg aaagaagacc agtcaacaca gccgcaataa gcaatttatt
     2341 tttcatttaa actctcaata aaagtagtat agcttagtta agctatttct atgtcagtgt
     2401 taatgttaat gtatataaat aatacttctc aacgactcac tgtttatata caccatgctg
     2461 tttagttagt ttattaagga cagcgagatt tttgttgtat tctcgcgtat tttgggcagg
     2521 gtaaccaaaa aggtcactct tttcaggaca agaatggctt acacctgatt tagccttaat
     2581 taccgaataa ctaccaattg atatgtgacc ggcgattccg acttgaccat gtgtaataac
     2641 atggtcgcct aaaactacgt gtcctgaaaa accgcattgt gaaacaatca ggcatttatt
     2701 accgattatg cagtcatgtc caatctgcac ttgattatca atacgagtgc cttggcctat
     2761 aatcgtatct ccaagtgtcc ctcggtcaat ggtgttgtta caaccgattt caacatcatc
     2821 acctatgatt actctaccga tgctatcaac tcggacatat gaatcacgtt catccgacat
     2881 atattcaaaa ctataattac cgatggagtt attagaatct atgataacgt cattgccaat
     2941 aatagtacct tctttaatca ctgtattcgc atgaatggca acattatcac ctatggtcac
     3001 acagttcata attttaacac caggcataaa atggcagttc atacctatct ggcagtgttt
     3061 accaatgtat acatcaggta tcgttgaagt gttttcttgc tcaaacaaac gatacttatg
     3121 taccttaaaa tatcttaaaa ctttgttaag attattcacg ttcaggtaat cgactacaat
     3181 ctttgcctta gcattagaac taatgatctc ttccggtccg ataattatat ccgcaccagt
     3241 cgttggtata tgcaacaaat ctccaggtgt cataacaatc gagagtccac caactaaagg
     3301 actatggatt ggccgaatgg tatcaaccct gagtgatgga tcaccatata aagttccttg
     3361 catcaacgtt ttaatatcta gtgcgctgaa cataggtttc cctctgttta atcaaaagcg
     3421 atagaaagca tgaacataaa tattatcctg attgagcaca ttatattttt cttcatctct
     3481 tggatcataa aagtttttgt gctctttttc ccaggcatgt ttatattcaa tcttggctat
     3541 ccagtcgtca cctatttttt gttcataacc tagagtggct tttcgaattg ttttgaaata
     3601 ggttctgttt gcattgtatt gcaaactatc tgcgttttct gttttcccgt gtccgaagcg
     3661 ggtgcgtaga tacagattac ctgcgttttc aaaagcatat ttgataattg gctcgtagta
     3721 agtttcggta taaatactac gatcatttat tttaccgtga ttaaattcat cgttatgctt
     3781 tttctggtag aaaaattcaa taccaaactc ccatgcacca aaacgtttat agggctgaaa
     3841 tagtatactt tgaccttctt cttcgcgtac gccccaggag ctcttatcat ttcgactgta
     3901 taagtattca acgtttgaat aaaaacctgc gttccagtca gagttaaagt acgtcaacgt
     3961 agggcgaaaa ctgttgtcaa cagttacagt atgcaagcct gtaacataag ggctatctat
     4021 atttccgtcg gtatacatca ggtcgtatac cagactgaag tcgaaaccgt taccaatagt
     4081 gaacgttttg tttagtgata tatagttttg cagacttgtt tcattttcgt gatagccttt
     4141 ttcttcttgc cggcgatcga tagacttaag gccatagatt gctgcaaatt gccaattagg
     4201 ttggctgaaa taggcctgga aaatctcgtg tgttttagta tattccttta ctgttttacc
     4261 taaatagtta tcagtgcttt tggtctcata ttcaaatgta gtaccaacat gtccattaaa
     4321 gcccgcttgt tcttctgacc agtcagtgtc ggaggtaaag ccgcgatcgt ttgccgcaaa
     4381 ggctcctgtt gagcctaaaa ggactgttgc aattgaagcc gataagacat tcttttttat
     4441 taaaaccata aatgagttcc ttccaaaata tcaatttaag atatgatttc tgttattcca
     4501 tatagtcgta cctcatgtac tcctaaacac tctgcggcaa taaaatattt ttttcctcaa
     4561 caaattactg caaatgagat atgcatcact atttgcagtt attcatcatt aaaatcttat
     4621 ttgacagttg attggccttg atttgtgttg ataatggcgt gaatgcttat gtaggcgtac
     4681 agcatgtact acttaaagat attattcacg aggtaaatat aatgagactt tcaaatatcg
     4741 tcacagttat aattcttgca atttccagta cgctaacacc ccaggcgatg gcagaaaaat
     4801 tgattcctga aactgatgct gagttattaa tctggtctga tgccacaagc gcggaatata
     4861 tgaaatatgc agcaacggaa ttcaataaag attttggcta caaggtcaag tttacatttc
     4921 gcaatatagc gccaatggat gcagcatcaa gaattatgca ggatgggggt acgactcgtg
     4981 tagctgatgt agctgaaatt gaacatgata ccctggggcg gttagtcgtt gctggcgggg
     5041 ttatggaaaa catggtctca gctgagcgga ttaaaaaaac atttattcca ggcgcagtat
     5101 cggcagctac atataataac atcagctatg gttttcctgt aagtttcgca acgctagcgc
     5161 ttttctataa taaggatttg ttaaacaccg caccaaaaac attcgaagaa atcaatactt
     5221 tcagtgaaaa gtttaataat tcatccgagc ataaatatgc tctgctatgg gatgtacaaa
     5281 attattatgt ttcacgtatg tttattacct tgtatggtgc caacgaattc ggtaaaaccg
     5341 gtaacgatcc taaagctcta ggcatcgctt catctgaagc gaagaaaggg ctagagacaa
     5401 tgaaacgctt aaagaaagcg aatccttcta atcctcttga tatgggtaat ccacaagttc
     5461 taagaggtct gtttaatgaa ggaaaagttg ctgctgtaat cgacggacct tggtccatac
     5521 aaggttacat tgacagcgga atcaattttg gcgtgacacg catcccaaca ttagatggtc
     5581 atcagcctcg cacattttca acagtacgac tggccgttgt aagctcattt accgaatatc
     5641 ctcatgccgc tgaattattc gctgactacc tgacaacgga taagatgttg atgaagcgtt
     5701 atgaaatgac gaatctcatc ccgccaatcg attcacttat gaacaaaatt agccagacag
     5761 gtagtgaagc tataaaagca attattgctc aagctaatta ttctgacgca atgccatcaa
     5821 taccggaaat gtcttatcta tggtctccaa tgactaatgc tattttggct acctgggttg
     5881 agaataaaac accggatgaa gttttaaatc atgcacaaac aattattgaa gaacaacttt
     5941 cgcttcagga gtaattgtga atacacaaca actgaaaatg aagagtgcac ctgtgctacc
     6001 tatttcctgt ttgattatgg gcgggactca gttatcacgt cattattatg tgaaaggcgg
     6061 gatattcttt gcaatacaag tatgcttttt gctttatctt tccgatattg tgcatacatt
     6121 aataggtttg ttcacattag gtgatgtcgc gcaaattcga aaagggttga ctgttattca
     6181 gggtgacaat tctatattta tgcttgtaga gggcgtgata gcgaccatta ttgttggctt
     6241 attcgccact atttacatcc ttaatattaa agatgcgcgg aactcatctt attgccatct
     6301 gacttttaaa caacagttat ataagctcta tgaggataaa tttgcattta tagtcctaac
     6361 gcccgcattt ttagcgagta tagcattcat tgttttacct attgtaatta cagttcttgt
     6421 atcgtttaca aactatgctg cgccaaatca catcccacca aagaacctgg ttgat
//
LOCUS       DAZUFB010000182         6379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZUFB010000182 DAZUFB010000000
VERSION     DAZUFB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.182"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..72)
                     /locus_tag="ACHY3M_004762"
     CDS             complement(<1..72)
                     /locus_tag="ACHY3M_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319520.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRV"
     gene            complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHY3M_004763"
     CDS             complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHY3M_004763"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFS4319521.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHY3M_004764"
     CDS             complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHY3M_004764"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFS4319522.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHY3M_004765"
     CDS             complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHY3M_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFS4319523.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4171..6204
                     /gene="betT"
                     /locus_tag="ACHY3M_004766"
     CDS             4171..6204
                     /gene="betT"
                     /locus_tag="ACHY3M_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFS4319524.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1318 a         1747 c         1744 g         1570 t
ORIGIN      
        1 aacacgaacc tttttcttca gataaaataa aatctcaatc agatcgatat cctgtttgat
       61 ttgttcacgc ataatatatc cagaaaataa aatctgtcgc agataaggtt gtattaatag
      121 tctgtatcag gaatgttcgg gttaaatatc agcaaaaagc cagcatcatg gatactggat
      181 atgaagcatg agagttacct cagtgtttat ataaggattc ggtccccctc tctggaacgg
      241 taactctcaa tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc
      301 accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca
      361 cgaatcatat ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg
      421 ataatctgcg gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg
      481 ccttcgccgt caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg
      541 aaggcggttt cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc
      601 gtgccggggc tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg
      661 cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta
      721 aacagaatcg ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt
      781 ggcgagcgca ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg
      841 ttatagttaa tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca
      901 cggctgcgaa taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca
      961 aacagccact ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa
     1021 accggttctt tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg
     1081 acgccgggta attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg
     1141 acgccggagc gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct
     1201 ttgttggccg ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc
     1261 gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatagcgt gaatacgaat ggtcaggtta
     1321 gggcgcgatt tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc
     1381 gtgacggtgc gatccatcgg accaaaacct tcctgctgat aaccgttgag atcgtccgtg
     1441 cgcgggtagc ccgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg
     1501 ggtttggagg tagtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca
     1561 tcgcgagtct cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga
     1621 ccgggttctt gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg
     1681 ttgatcagcg acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc
     1741 ataaacggtt caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac
     1801 gccagcgctg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca
     1861 agcagcagca cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct
     1921 gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga
     1981 ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat
     2041 cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg
     2101 ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg
     2161 cgcgcggttc aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc
     2221 gcggcgaatc acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat
     2281 ctcttcgcgc acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca
     2341 tgcgccgtta tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc
     2401 ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag
     2461 cgggccgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc
     2521 cagaattttc tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccgtt
     2581 ggtacacacc tgaccggagc tgaagaagtt cgccatcatg gcgatatcgg cggcgagatc
     2641 gagatccgca tcgtcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc
     2701 tttcagggaa gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt
     2761 aaatgacact ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgccccc
     2821 ccccggcaac acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag
     2881 ctttaacgcg gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc
     2941 cagcgccggg gcggatttcc acagggcaat ctggatcggg tagttccatg cgccaatccc
     3001 cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat
     3061 ctggctgcct tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt
     3121 aacgatatcg acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt
     3181 tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg
     3241 ctccatagcg gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg
     3301 atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt
     3361 aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg
     3421 ctgttctgcc attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa
     3481 taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cggtttgccg ctcagtgccg
     3541 cgcgcagcca taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt
     3601 ggcgcggtaa ctcgcggcga aattcgctca ccagattcga caataagcgg cggctactga
     3661 cctgctgtaa acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg
     3721 ctttcatcgc cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta
     3781 atcgctgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca
     3841 gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt
     3901 ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt
     3961 gcatgcccac ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg
     4021 actgcatccc caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa
     4081 agcggttatt gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt
     4141 aaatggtaac aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag
     4201 gacaaaatca atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg
     4261 acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt
     4321 tctaaaacct tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc
     4381 tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa
     4441 ttcagcctgc tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg
     4501 ttcttctccg tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag
     4561 acgattgagg ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc
     4621 tggtcgatgt atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg
     4681 ccgctcacca tccgctcggc gctgtacccg atcttcggta aacggattaa cgggccgata
     4741 gggcactcag tggatattgc agcggtgatc ggcaccatct tcggtattgc cactacgctc
     4801 ggtatcggtg tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg
     4861 atggcggcaa aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc
     4921 tccggtgtcg ataaggggat tcgtgtgtta tcggaactta acgttgcgct ggcgctggga
     4981 ttgatcctgt tcgtattgtt tatgggcgac acctcgttcc tgcttaatgc gctggtgctg
     5041 aatgttggcg actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac
     5101 cgtccagttg agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg
     5161 tcgccgtttg tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc
     5221 gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc
     5281 aatagcgcgc tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc
     5341 catccggagc gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc
     5401 tccgtcgcca ccattactgg cctgctgttt tatgtgacct cggcggactc cggtgcgctg
     5461 gtgctgggga atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg
     5521 cgcgtcttct ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg
     5581 atatccgcgc tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc
     5641 ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc
     5701 aaccgcgata ccgcaccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt
     5761 ctctcgcgcc tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc
     5821 tgttacccgg caatggaaga agtggcgcag gagctgcggt tgcgcggcgc atacgtggag
     5881 ctaaaaagcc tgccgccgga agagggacaa cagttggggc atctggattt gttggtgcat
     5941 atgggcgaag aacaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc
     6001 tttacctacc gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta
     6061 gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt
     6121 cttgaccagt acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat
     6181 agcgtgatgt tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg
     6241 gcgcgggaat cattgactcg ctgtaaaaaa ttatgcatat attatatatg attgtcatgt
     6301 catatcctgc aattcctctt gtatcacttc atgattttat attttattaa ttaacgattt
     6361 tattttgtat tagctgaat
//
LOCUS       DAZUFB010000183         6371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZUFB010000183 DAZUFB010000000
VERSION     DAZUFB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.183"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..648
                     /locus_tag="ACHY3M_004767"
     CDS             <1..648
                     /locus_tag="ACHY3M_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFS4319525.1"
                     /translation="SPKDASAWRAGKADYAKMATHATQTRLKNVLNKGDLTPEAVNTI
                     VYGQYGSDIARLYGKLDQKGKDMLRAAYISKIADKVGDSPQKMMTELGKLQKQANGQV
                     FKTVFGGKNGKEIEGMLSILDATKRASEANVVTKTGMTLAPLVRVIGNLKTGGALLAG
                     ETGIGLMSRVYESPMARNALLRLANTKAGTPAYERALSNAANAIRPLLATEATQQ"
     gene            complement(915..1556)
                     /locus_tag="ACHY3M_004768"
     CDS             complement(915..1556)
                     /locus_tag="ACHY3M_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319526.1"
                     /translation="MNFDDQFPSRVSLARQSRGMTQAQLSKLAGVVQRQIAAYEGGEA
                     KPRLRVLQALANALGTTAEWLALGEGQGPGTKNVMPDVLVKQIPILKLDEVMHYLNTG
                     EHSSSRFHPAIYNVGDSAFALTIEGEAMTTSSGISFPRGSVVTFSPLVKAKSKDYVIA
                     SLDKEQILSFKQVYIGEIETNLVSLNPMYPNILVRNEDVSILATAVYLEIPLL"
     gene            1656..1829
                     /locus_tag="ACHY3M_004769"
     CDS             1656..1829
                     /locus_tag="ACHY3M_004769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319527.1"
                     /translation="MTEKISSIKPRQVRFTEKVDSHIRESAKRCHRSIQAEIAYRMEL
                     LMKLEAKGDVVIQ"
     gene            1903..2529
                     /locus_tag="ACHY3M_004770"
     CDS             1903..2529
                     /locus_tag="ACHY3M_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFS4319528.1"
                     /translation="MNIVAKSDYNFHGVELVPTRDMHGVWFTSSNIASALKYANSRAV
                     TMIYNKYSDEFSTGMTQVLEVSTSGNYRKKVRVFSLRGAHLIAMFARTPVAKEFRRWV
                     LDILDRQAECSPIAKQFTDEELVNLCYLQLWMEKSQQMCKHIYPGMKQIGSELSGRIY
                     DIAYETRYMSEETKKSLLREMKNLDTNNFVVKNAQPMLAKLRGEEWIH"
     gene            2618..3346
                     /locus_tag="ACHY3M_004771"
     CDS             2618..3346
                     /locus_tag="ACHY3M_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319529.1"
                     /translation="MTFKNVANIGSVVTDKTIDSQYLLEMVNHARRQRGEKEVRNNDF
                     IARIKDELEGEHYEIFVVQKSNKTTSEKVVMSIKQALRVAARESKAVRRSLVDQLESM
                     QEAHIKSGKSSSGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVG
                     FNAIPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQ
                     VEAFRYNAEGVKALQHLIHGSNVA"
     gene            3346..3567
                     /locus_tag="ACHY3M_004772"
     CDS             3346..3567
                     /locus_tag="ACHY3M_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319530.1"
                     /translation="MAKKRYGIMPPRIKGRARVKGDAGRYHILGVLWHERALILSRPH
                     GYIEKVSIDRVEILPLTPEEEETYGLFDN"
     gene            complement(3582..3869)
                     /locus_tag="ACHY3M_004773"
     CDS             complement(3582..3869)
                     /locus_tag="ACHY3M_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4319531.1"
                     /translation="MPFHPFGEALMMYSKYDEAQFHLRLTHELHAKIKQRAKMNNRSI
                     NSEIVATMEESLSKPSPVSGYRDEEERLASLISERVKEVAADILRKEKTRD"
     gene            3972..5909
                     /locus_tag="ACHY3M_004774"
     CDS             3972..5909
                     /locus_tag="ACHY3M_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFS4319532.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGSQQFY
                     FPNVLKYDPDQLRQELAGPNGYLLIPSMDQHIKIQQWREEGDIRGWGAIDGEFNDAAV
                     SAALDSESPSVKLGGVGFVSKLRSPINHKSNKVMHSGSLNFQFDGGTQQEKSGILMAN
                     ISNAKVIDVDITGTLDGGIRGYGGSNIVIDGVNVHDIGISTLSGECGIGIWFGDYANY
                     DVQTDGLLIQNCNIKNIGGVGIQRGDGILVYNAKNFKVRYNTIITTNRMAIAAGSDTR
                     QFEIHGNYIGDTLLAGIDIEPDEGYTASNFKIYNNNIIGFAARYFTQGSGVGQTFGID
                     THANTSYGKVYKNTLSAGQYGTEAFHIGNHADEIEVTDNNLIGGAVVIPLFIKTYDGS
                     GSKHIKINRNRAKGTCKSFADVAMSEDVYISENVFSGNSSNDSYFLRASTIAGLNVDY
                     NRSSNTTNFIKSGDAGNTSSVKVTNNNASTLSDGIDMLTSGSMTGFIASGNTILCPAS
                     NKGISLEVAGSGSISDISLRGNIIYNATTKIYVSPAATGWDMLTKNTRFDLSGVQNGT
                     QLFELSRNRVTQFLNNAWYDG"
     gene            complement(5950..>6371)
                     /locus_tag="ACHY3M_004775"
     CDS             complement(5950..>6371)
                     /locus_tag="ACHY3M_004775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4319533.1"
                     /translation="YSNTKTNNKLLNKAIKMLAPIVAVISVWGMLSPSMWMGEMQWAI
                     PCFGLVTSLSLLEKSGVSFEFPSLVKIGAMSFSIYLIHPIIIELLSQKYFVVFWQDGF
                     TKFSVIILITVFAARIMYETIEIPSQKLARKLISKIR"
BASE COUNT         1975 a         1278 c         1399 g         1719 t
ORIGIN      
        1 agcccaaaag atgcttcagc atggagagcg ggaaaagctg attatgcaaa aatggcaaca
       61 catgcaactc aaacgcgcct taaaaacgtt ctaaacaaag gagatttaac tcccgaggct
      121 gtaaatacca ttgtgtatgg acaatatggg tcagatatag ctcgattgta cgggaaactc
      181 gatcaaaaag gtaaagacat gctaagggcg gcatatatca gcaaaatagc tgacaaggta
      241 ggtgacagcc ctcagaaaat gatgaccgag cttggcaagc tgcaaaaaca agcaaatggt
      301 caggtgttta aaactgtatt tggtgggaag aacggaaaag agatagaggg gatgttatct
      361 attctcgatg ctaccaaaag agcatctgag gctaatgttg tgacgaagac tggcatgaca
      421 ctcgcgcctt tggtaagggt tattggtaac ctaaaaaccg gaggcgcgct attggctggg
      481 gaaacaggga ttggccttat gtcgagggtt tatgaaagcc ctatggccag gaatgcgctc
      541 ttacgtctgg caaacactaa agcaggaacg ccagcctatg aaagagcatt aagtaacgct
      601 gcaaatgcca tcagaccgct gcttgccact gaggcaacac agcagtgact aaatgccatg
      661 gatggttatt tccttagcac atgaaacaat gtttgcttta attccaccca tacaattatg
      721 accactatag acagacaaaa gaaactgaat gcattggcat cacgatcgaa accttctccg
      781 gcactaaatc cgtaaaaggt cataaaaaat ataaaaattg cgcactttgc aacgtttaca
      841 aattttttct tcacactaac ctccttagtt ttgagcagga taccagatga taatgtgtcg
      901 cttcctaaag gatatcaaag caacgggatt tcaaggtaaa ctgcggttgc caaaatacta
      961 acatcttcat ttctaacaag aatattagga tacattgggt ttagggatac taggtttgtc
     1021 tctatttcac caatgtaaac ctgtttgaag ctcaatattt gctctttatc caatgatgct
     1081 attacataat ctttgctttt agcttttact agagggctga acgtgacaac cgatcctctg
     1141 ggaaagctaa tacctgaact tgtagtcata gcttcacctt caatagtcaa tgcaaatgca
     1201 gagtcaccaa cattgtatat tgccgggtga aatctagacg atgaatgttc acctgtgttt
     1261 aggtaatgca taacttcatc tagtttgagg attggtattt gctttaccag aacgtcaggc
     1321 atgacgtttt ttgttcccgg accctgacct tcacctagag ctaaccactc agctgtagta
     1381 cctaatgcgt tggctaatgc ctgcaatacc cgaagccgtg gtttagcctc accaccctcg
     1441 tatgcagcta tttgacgttg aacaacacca gctaattttg acaactgcgc ctgcgtcata
     1501 ccccttgact gtcttgccag agatactctt gatgggaatt gatcgtcaaa attcattagt
     1561 tcaccataaa aaattcattg actcatgctg agtgtgagtg aataatcaaa ctacaaaaag
     1621 tgagattatg agttttttaa aaacaggagt gcataatgac tgaaaagata tcttccatca
     1681 agccgcgtca ggttcgtttt acagaaaagg ttgattcaca tatccgcgaa tcagcaaaaa
     1741 gatgccatag gtcaattcag gcagagatag cttatcgaat ggagttattg atgaaacttg
     1801 aggcaaaggg cgatgttgtc atccaataaa aatagtgaag cccggcagtg cgcgaacaca
     1861 aaccgggcct ctatgtcagt aaccgtatgc aaggaaacta acatgaatat tgtagcaaaa
     1921 tcagattaca acttccacgg agttgagttg gtgcccaccc gtgatatgca tggtgtttgg
     1981 tttacgtcat ctaatattgc atctgcactt aaatatgcaa atagccgtgc agtaacaatg
     2041 atttataaca agtatagcga tgagtttagc accggaatga ctcaggtact cgaagtgagt
     2101 acctcaggaa attatcgcaa aaaagtgcga gttttctcac tacgcggcgc ccacctaatc
     2161 gcgatgtttg ctcgcactcc ggtagccaaa gagttccgcc gctgggtgct ggatattttg
     2221 gatcggcagg cagaatgctc accgattgca aaacagttta ctgacgaaga gctggttaat
     2281 ctctgctact tacaattgtg gatggagaag agtcaacaaa tgtgcaaaca catctaccca
     2341 ggaatgaagc aaattggttc tgagctttct ggaaggattt acgatattgc atatgagact
     2401 cgctacatgt cagaagaaac caagaaatca cttcttcgtg aaatgaagaa tcttgatacc
     2461 aataattttg tcgtaaagaa cgctcagcca atgctggcaa aactgcgcgg cgaggaatgg
     2521 attcattgat tggtgcgccg gacggcgcaa aaagaaaacc gccagtgtgc tgctggcggc
     2581 ctatgtcaca cccttactac cacataagga atgcctaatg acttttaaga atgtagcaaa
     2641 catcggatcc gttgtcacgg ataaaaccat tgatagccag tacctgttag agatggtcaa
     2701 tcatgctcgt agacagcgcg gggaaaaaga agtccgcaat aacgacttta ttgcacgcat
     2761 taaggatgaa cttgaaggtg agcactacga aattttcgta gttcaaaaat caaacaagac
     2821 aacttctgaa aaagttgtta tgtcaattaa gcaagccctt cgagtggctg ctcgtgaatc
     2881 taaagctgtt cgccgttcac ttgtagacca acttgaaagt atgcaagaag cgcacattaa
     2941 aagcggtaaa tcgtcgagtg gacttgttga gtatcgccag gcgcgaacat tgaaaatgac
     3001 ggttgaagct gttaccaatc tgttcgattt gatgccaaat cttgcgccgg aagcaaagca
     3061 gactgcggca gcaagcataa tcaacccgct cgttggtttt aatgcaatac ctcttccggc
     3121 aatagaagag cattactact cagcagggga ggttgcagag cagcttggag taacggccaa
     3181 caagattggt cgcattgcta acgcaaacaa cctcaaaact gagcagtacg ggaagttctt
     3241 cttggataaa tctgcgcatt caagcaaaca ggtggaagca ttccgctaca atgcggaggg
     3301 tgttaaagca ctacaacacc tgattcatgg gagcaatgtt gcataatggc aaagaaaaga
     3361 tatggcatta tgccgccaag aatcaaagga agagccaggg taaaaggaga tgctggaagg
     3421 tatcacattc ttggagttct gtggcatgag agagctttaa ttttaagtag acctcatggg
     3481 tacattgaaa aggtatctat agatagagta gagattcttc cccttacacc tgaagaagaa
     3541 gaaacttacg gactttttga taactaacca attaaacccg cttaatcgcg ggttttttct
     3601 tttctaagaa tatcagccgc aacttctttt actcgttccg agattaatga ggcaagcctc
     3661 tcttcttcat cacgataccc gcttacaggt gatggtttgg agagcgattc ttccatcgta
     3721 gccacaattt cggaattgat agacctgtta ttcatttttg cacgttgctt aatcttggcg
     3781 tgcaactcgt gcgtaagcct caagtggaac tgcgcctcat cgtatttgct gtacatcatc
     3841 aatgcctcac caaatgggtg gaatggcatc gtaaaaccta ctgtacaaat caacaatcgt
     3901 accgtttcgg tatgcaacaa ataccaaccg tagccatgct gcggcgattc cttgtatctg
     3961 gagcaaatta aatgacagac attacagcca atgtgatcgt atcgatgcct tcgcaactct
     4021 tcactatggc tcgttctttt aaagccgtag ccaatggcaa aatttatatc ggtaaaattg
     4081 acactgaccc ggtaaatcct gaaaaccaga ttcaggttta tgtggagaat gaagacggtt
     4141 ctcacgttcc tgtttcgcaa ccaataatca ttaacgctgc tggatatccg gtatataacg
     4201 gacagattgc caaattcgta accgtgcaag gccattctat ggctgtttat gatgcgtatg
     4261 gttcccagca gttctatttt cctaatgtgc tgaagtatga ccctgaccaa ttaagacaag
     4321 agctagcggg tccaaatgga tatcttctaa tcccatcgat ggatcagcat attaaaattc
     4381 agcagtggag agaagagggt gacattcgcg gatggggtgc tattgatgga gagttcaacg
     4441 atgctgctgt atccgctgca ttagactctg aatctcctag cgttaaactt ggtggcgtcg
     4501 gattcgtttc aaaacttcgc tcacctatta accacaaaag taataaagtg atgcatagcg
     4561 ggtcattaaa ctttcaattt gatggcggaa ctcagcaaga gaaatccggg atacttatgg
     4621 ctaacatctc caacgccaaa gtgattgatg ttgatataac aggcaccctg gatggaggaa
     4681 ttcgtgggta cggtggcagc aatatcgtta tagacggcgt gaatgttcac gatattggaa
     4741 tttcaacgct atccggtgag tgcggcatag gaatatggtt cggtgattat gcaaactacg
     4801 atgtacagac ggatgggctg ttaattcaaa attgtaatat caaaaatatt ggaggtgtag
     4861 ggatacagcg tggggatggc atcctggttt ataacgcgaa aaatttcaag gtaaggtata
     4921 acaccatcat tacaaccaat aggatggcta ttgcagctgg aagtgatact aggcagtttg
     4981 agattcatgg aaactacata ggtgatacgc tattagctgg tatagatatt gagcctgacg
     5041 aaggttacac tgcttcaaat ttcaagatat acaataacaa tattatcgga tttgctgcac
     5101 gatacttcac tcaaggatct ggtgttggtc aaacctttgg tatagatact catgctaata
     5161 cctcatatgg caaagtgtat aaaaacacat tgtctgcagg acagtatgga acagaagcat
     5221 ttcatatagg aaaccatgcg gatgagattg aagttacgga taacaacctg attggcggtg
     5281 ctgttgtaat cccattattc atcaagacat atgatggtag tggtagtaaa cacatcaaaa
     5341 taaaccgcaa cagggcaaaa gggacgtgta agtcatttgc agatgtagcc atgtctgaag
     5401 atgtttatat ttctgaaaat gtattctctg gcaatagttc taatgatagt tatttcctga
     5461 gagcgtcaac aatagcaggg cttaatgttg actacaaccg gtcaagcaac acaaccaatt
     5521 tcatcaagtc tggtgacgct ggaaacacgt caagcgtgaa ggtcactaat aataacgctt
     5581 ctaccctttc agatggtata gacatgctaa cgtcgggttc catgactggt tttattgctt
     5641 ctggcaacac tattttatgt ccagcatcca acaaaggaat ttcactggaa gtagcaggtt
     5701 caggttctat ttcagacatt agtctccgtg gcaacataat ttataatgct acaaccaaaa
     5761 tatatgtatc accagctgcc acgggatggg atatgttaac caaaaatacc aggtttgatc
     5821 tgtctggagt tcaaaatggc actcagctat ttgaactatc aagaaataga gttacacagt
     5881 ttcttaacaa tgcttggtat gatggctagg cgacctgcga taccacaaat ttaatggtat
     5941 cgcagttttt catcttattt ttgatatgag ctttctggct agtttttgtg atggaatctc
     6001 aattgtttca tacattattc ttgccgcaaa aactgtaatc aaaatgatga ctgagaactt
     6061 agtaaatcca tcctgccaaa aaacaacaaa atatttttgg cttaataatt cgataattat
     6121 tgggtgtatt agatatattg agaatgacat tgctccaatt tttacaagac ttggaaattc
     6181 aaaagaaacc ccagacttct ccagtagtga caaagaggtt acaagaccaa agcaaggtat
     6241 cgcccactgc atttcaccca tccacattga aggtgatagc atcccccaga ctgatattac
     6301 ggcaactatc ggagctaaca ttttaattgc tttgtttaaa agtttgttgt tggtttttgt
     6361 attgctataa a
//
LOCUS       DAZUFB010000184         6106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZUFB010000184 DAZUFB010000000
VERSION     DAZUFB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.184"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(115..549)
                     /gene="merR"
                     /locus_tag="ACHY3M_004776"
     CDS             complement(115..549)
                     /gene="merR"
                     /locus_tag="ACHY3M_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS4319534.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            621..971
                     /locus_tag="ACHY3M_004777"
     CDS             621..971
                     /locus_tag="ACHY3M_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFS4319535.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            985..1260
                     /gene="merP"
                     /locus_tag="ACHY3M_004778"
     CDS             985..1260
                     /gene="merP"
                     /locus_tag="ACHY3M_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFS4319536.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1296..1718
                     /gene="merC"
                     /locus_tag="ACHY3M_004779"
     CDS             1296..1718
                     /gene="merC"
                     /locus_tag="ACHY3M_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFS4319537.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1770..3464
                     /gene="merA"
                     /locus_tag="ACHY3M_004780"
     CDS             1770..3464
                     /gene="merA"
                     /locus_tag="ACHY3M_004780"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS4319538.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3482..3844
                     /gene="merD"
                     /locus_tag="ACHY3M_004781"
     CDS             3482..3844
                     /gene="merD"
                     /locus_tag="ACHY3M_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFS4319539.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3841..4077
                     /gene="merE"
                     /locus_tag="ACHY3M_004782"
     CDS             3841..4077
                     /gene="merE"
                     /locus_tag="ACHY3M_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFS4319540.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4074..4721
                     /locus_tag="ACHY3M_004783"
                     /pseudo
     CDS             4074..4721
                     /locus_tag="ACHY3M_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4856..>6106
                     /locus_tag="ACHY3M_004784"
     CDS             4856..>6106
                     /locus_tag="ACHY3M_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4319541.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYAD"
BASE COUNT         1092 a         1968 c         1942 g         1104 t
ORIGIN      
        1 cggcctcacc ccaaaaatgg cagcgcctac ggcgctgact ctttcgttga ccaggcgcaa
       61 gcgcctggaa atgctcaatt agggggcacc tcagaaaacg gaaaataaag cacgctaagg
      121 catagctgac cttgccaggc ctgcttcgcc ctgtagtgac gcgatcaacg ggcaggaaac
      181 attccccttt cgtgcatggc aggcgcacac gagttcagac agcacggttt ccatgcgcgc
      241 caagtcggcc atcttctcgc gcacgtcctt gagcttgtgt tcggccaggc tgctggcctc
      301 ctcgcagtgg gtgccatcgt cgagccgcaa cagctcggca atctcgtcca gactgaaccc
      361 cagccgctgt gccgatttca cgaatttcac ccgaaccacg tccgcctccc catagcggcg
      421 gatgctgccg taaggcttgt ccggttcccg caacaggccc ttgcgctgat agaagcggat
      481 tgtctccacg ttgaccccgg ccgccttggc aaaaacgcca atggtcaggt tttccaaatt
      541 attttccata tcgcttgact ccgtacatga gtacggaagt aaggttacgc tatccaatcc
      601 aaattcaaaa gggccaacgt atgtctgaac cacaaaacgg gcgcggtgcg ctcttcgccg
      661 gcgggctggc cgccattctt gcatcgacct gctgcctggg gccgctagta ctggtcgccc
      721 tgggcttctc cggtgcttgg atcggcaacc tgacggtgct ggaaccctat cgaccgttgt
      781 tcatcggcgc ggcgctagtg gcgctgttct tcgcctggaa gcggatttac cggcccgtgc
      841 aggcatgcaa gccaggtgag gtctgcgcga ttccgcaggt gcgcgccacc tacaagctga
      901 ttttctggat cgtggccgtg ctggtcctgg tcgcgcttgg atttccctat gtcgttccat
      961 ttttctatta accaggagtt catcatgaag aaactgtttg cctcccttgc cctcgccgcc
     1021 gctgttgccc cggtgtgggc cgctacccag accgtcacgc tagcggttcc cggcatgact
     1081 tgcgccgcct gcccgatcac agtcaagaaa gcgctctcca aggtcgaagg cgtgagcaag
     1141 gtcgatgtgg gcttcgagaa gcgcgaggcc gtcgtcactt ttgacgacac caaggccagc
     1201 gtacagaagc tgaccaaggc caccgcagac gccggctatc cgtccagcgt caagcagtga
     1261 gccagcaagc caacgacaac agcgagagcc gcttcatggg actgatgaca cgcattgccg
     1321 ataaaaccgg cgcgctcggc agcgtcgttt ccgcgatggg ctgcgccgcc tgctttccag
     1381 ccctcgccag cttcggcgcg gccatcgggc tgggcttctt gagccagtac gagggactgt
     1441 tcatcagccg cctgctgccg ctgtttgccg cgctggcctt cctggcgaac gcgctgggtt
     1501 ggttcagtca tcggcaatgg ctgcgcagtc tgctcggcat gatcggcccg gccatcgtgt
     1561 ttgcggccac ggtctggctg ctcggcaact ggtggacggc gaacctgatg tacgtcggcc
     1621 tggccttgat gattggggtg tcgatctggg acttcgtgtc gccggcgcat cgccgttgcg
     1681 gaccggacgg ctgcgaactc cccgccaagc gcttgtgaaa gacggctgac cgtgcgacac
     1741 ggcggcccac acgaataagg aacgatggta tgagcactct caaaatcacc ggcatgactt
     1801 gcgactcgtg cgcagtgcat gtcaaggacg ccctggagaa agtgcccggc gtgcaatcag
     1861 cggatgtctc ctacgccaag ggcagcgcca agctcgccat tgaggtcggc acgtcacccg
     1921 acgcgctgac ggccgctgta gctggactcg gttatcgggc cacgctggcc gatgccccct
     1981 cagtttcgac gccgggcgga ttgctcgaca agatgcgcga tctgctgggc agaaacgaca
     2041 agacgggtag cagcggcgca ttgcatatcg ccgtcatcgg cagcggcggg gccgcgatgg
     2101 cagcggcgct gaaggccgtc gagcaaggcg cacgtgtcac gctgatcgag cgcggcacca
     2161 tcggcggcac ctgcgtcaat gtcggttgtg tgccgtccaa gatcatgatc cgcgccgccc
     2221 atatcgccca tctgcgccgg gaaagcccgt tcgatggcgg catcgccgct accacgccga
     2281 ccatccagcg cacggcgctg ctggcccagc agcaggcccg cgtcgatgaa ctgcgccacg
     2341 ccaagtacga aggcatcttg gagggcaatc cggcgatcac tgtgctgcac ggctccgccc
     2401 gctttaagga caatcgcaac ctgatcgtgc aactcaacga cggcggcgag cgcgtggtgg
     2461 cattcgaccg ctgcctgatc gccaccggcg cgagcccggc cgtgccgccg attcccggcc
     2521 tgaaagacac tccgtactgg acttccactg aagcgctggt cagcgagacg attcctaagc
     2581 gcctggccgt gattggctca tcagtggtgg cgctggagct ggcgcaggcg ttcgcccgac
     2641 tcggagcgaa ggtgacgatc ctggctcgca gcacgctgtt cttccgcgaa gacccagcta
     2701 taggcgaagc cgtcacggcc gcattccgca tggagggcat cgaggtgagg gaacacaccc
     2761 aggccagcca ggtcgcgtat atcaatggtg aaggggacgg cgaattcgtg ctcaccacgg
     2821 cgcacggcga actgcgcgcc gacaagctgc tggtcgccac cggccgcgcg cccaacacac
     2881 gcaagctggc actggatgcg acgggcgtca cgctcacccc gcaaggcgct atcgtcatcg
     2941 accccggcat gcgtacaagc gtggaacaca tctacgccgc aggcgactgc accgaccagc
     3001 cgcagttcgt ctatgtggcg gcagcggccg gcactcgcgc cgcgatcaac atgaccggcg
     3061 gtgacgcggc cctgaacctg accgcgatgc cggccgtggt gttcaccgac ccgcaagtgg
     3121 cgaccgtagg ctacagcgag gcggaagcgc accatgacgg catcaaaact gatagtcgca
     3181 cgctaacgct ggacaacgtg ccgcgcgcgc tcgccaactt cgacacgcgc ggcttcatca
     3241 aactggtggt tgaagaaggc agcggacgac tgatcggcgt gcaggcagtg gccccggaag
     3301 cgggcgaact gatccagacg gccgcactgg cgattcgcaa ccggatgacg gtgcaggaac
     3361 tggccgacca gttgttcccc tacctgacga tggtcgaagg gttgaagctc gcggcgcaga
     3421 ccttcaacaa ggatgtgaag cagctttcct gctgcgccgg gtgaggacaa ggaggtgtgc
     3481 gatgagcgcc tacacggtat cgcaactggc ccataacgct ggggtgagcg tacatatcgt
     3541 gcgcgactac ctggtgcgcg gcttgttacg gccggtggcc tgcaccacgg gcggctacgg
     3601 cgtgttcgac gatgcggcct tgcaacggct gtgcttcgtg cgcgcggcct tcgaggcggg
     3661 tatcggcctg gatgccctgg cgcggctgtg ccgtgcgctc gacgcagcgg acggcgcaca
     3721 agccgcagcg cagcttgccg tgctgcgcca gttggtcgag cggcggcgcg cggcgttggc
     3781 ccatctggac gcgcaactgg cctccatgcc agccgagcgg gcgcacgagg aggcattgcc
     3841 gtgaacgccc ctgacaaact gccgcccgag acgcgccaac ccgtttccgg ctacctgtgg
     3901 ggtgcgctgg ccgtgttgac ctgcccctgc catctgccga ttctcgccgc cgtgctggcc
     3961 gggacgaccg ccggtgcctt ccttggcgag cattggggtg ttgccgcgct cgcgctgacc
     4021 ggcttgttcg ttctggccgt aacgcggctg ctgcgcgcct tccggggcgg atcatgacga
     4081 gttcgcagcc cgccggatgg acggcggccg agttggcgca ggcggcggcg cgcggacagc
     4141 ttgacctgca ttaccagccg ctggtcgatc tgcgcgatca ccggatcgct ggcgcggaag
     4201 cgttgatgcg ctggcggcat ccgaggcttg gcctgttgcc gcccggccag ttcctgccgc
     4261 tggccgagtc gttcggcctg atgccggaaa taggcgcgtg ggtgctgggc gaggcctgtc
     4321 gccagatgca caagtggcaa ggaccggcat ggcaaccgtt ccgtcttgcc atcaatgtgt
     4381 ccgccagcca ggttgggcca acgttcgacg acgaggtaaa gcgggtgctg gccgatatgg
     4441 ccctgcccgc cgagcttctg gagatcgaac tgaccgaatc ggtcgcattc ggcaatccag
     4501 ccctgttcgc cagtttcgac gccttgcgcg ccatcggcgt gcgcttcgcc gccgacgact
     4561 tcggcaccgg ctattcctgc ctgcaacatc tgaaatgctg ccccatcacc acattgaaaa
     4621 tcgaccaatc ctttgtcgcc aggctcccgg atgatgcccg tgaccaaact atcgtgcggg
     4681 cggtgatcca gctcgcgcac gggctgggca tggatgtcat tttcagaaga cgactgcacc
     4741 agttgattgg gcgtaatggc tgttgtgcag ccagctcctg acagttcaat atcagaagtg
     4801 atctgcacca atctcgacta tgctcaatac tcgtgtgcac caaagcgagg tgagcatggc
     4861 gacggacacc ccacggattc cagaacaagg cgtggccact ctgcctgatg aggcttggga
     4921 gcgtgcgcgc cgtcgtgcgg agatcatcag tccgttggcg cagtcggaga cggtcgggca
     4981 cgaagcggcc gatatggcgg ctcaggcgct gggcttgtct cggcgccagg tatacgttct
     5041 gatccggcgt gcccggcaag gcagcggcct cgtgacggat ctggtgcccg gccagtccgg
     5101 tggaggtaaa ggtaaggggc gcttgccgga accggtcgag cgcgtcatcc acgagctact
     5161 gcaaaagcgg ttcctgacca agcagaagcg cagcctagcg gcctttcacc gcgaagtcac
     5221 tcaggtgtgc aaggctcaaa aactgcgagt gccggcgcgc aataccgtgg ccttacggat
     5281 cgctagcctt gacccgcgca aggtcatccg ccggcgggaa ggccaggatg ccgctcgtga
     5341 cctacaaggt gtgggcggcg agcctcctgc cgtgaccgcg ccgctggagc aggtgcagat
     5401 agaccatacg gtcatcgacc tgatcgtggt cgatgaccgc gaccggcaac ctattggccg
     5461 cccgtacctg accctcgcca tcgacgtgtt cacccgctgc gtgctcggca tggtcgtcac
     5521 gctggaagcg ccgtctgccg tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa
     5581 gcgcccttgg ctggaaggac tgaacgtgga aatggattgg cagatgagcg gcaagccctt
     5641 gctgctctac ctagacaacg cggccgagtt caagagcgag gccctgcgcc ggggttgcga
     5701 gcagcatggc atccggctgg actatcgccc gctgggacag ccgcactatg gcggcatcgt
     5761 ggaacggatc atcggcacgg cgatgcagat gattcacgac gaactgccgg gaacgacctt
     5821 ctccaaccct gaccagcgcg gcgactacga ttccgaaaac aaggccgccc tgacgctgcg
     5881 cgagctagag cgctggctca cattggcggt cggcacctac cacggttcgg tgcacaacgg
     5941 cctgctccaa ccgccggccg cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc
     6001 cgtcgtcaca cgcgctactt cgttcctggt cgattttctg ccgatcctcc ggcgcacgct
     6061 gacccgcacc ggctttgtca tcgaccacat ccactactac gccgat
//
LOCUS       DAZUFB010000185         6074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZUFB010000185 DAZUFB010000000
VERSION     DAZUFB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.185"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..205)
                     /locus_tag="ACHY3M_004785"
     CDS             complement(62..205)
                     /locus_tag="ACHY3M_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFS4319542.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGV
                     ETQ"
     gene            complement(267..527)
                     /locus_tag="ACHY3M_004786"
     CDS             complement(267..527)
                     /locus_tag="ACHY3M_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4319543.1"
                     /translation="MEIKNSISDYTEAEFLQLVTVIYNADTASEEEQVKLVSEFERLT
                     EHPDGSDLIYYPRDDREDSPEGIVKEIKEWRSANGKSGFKQG"
     gene            complement(530..2260)
                     /locus_tag="ACHY3M_004787"
     CDS             complement(530..2260)
                     /locus_tag="ACHY3M_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFS4319544.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISV
                     SGEALSAAIADIFAALKGPFKFSAWGIALYGILPAEIAKDDPNMMSKIVTSLPAETVT
                     DAQVSSLPLDQATVSVTKRVTDVVKDERQHIAVVSGIPMSVPVVDAKPTNTPGVFSTS
                     FPGVPSLTVSTVNDAPVSTSLPVGVTEDKDRTAFPAGFTFGGGSHEAIIRFPADSGQK
                     PVYVSVTDVLTPEQIKQRQDEENRRQQEWDDTHPVEVAERNYEQARAELNRANEDVAR
                     NQERQAQAEQVYNSRKSELDAANKTFADAIAEIKQFERFAHDPMAGGHRMWQMAGLKA
                     QRAQTDVNNKQAAFDAAAKDKSDADAALGAALERRNQKENKEKDAKDKLDKESKRNKP
                     GKATGKGKPVGDKWLDDAGKDSGVPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPE
                     LSKNLSPSNKSGVSKGYSPFTPKGQQVGGRKVYELHHDNPISQGGEVYDMDNIRVTTP
                     KRHIDIHRGQ"
     gene            complement(2481..3014)
                     /locus_tag="ACHY3M_004788"
     CDS             complement(2481..3014)
                     /locus_tag="ACHY3M_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319545.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVT
                     LEDKLAEKPVTGKSRKN"
     gene            complement(3017..3943)
                     /locus_tag="ACHY3M_004789"
     CDS             complement(3017..3943)
                     /locus_tag="ACHY3M_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFS4319546.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFSIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPDRGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDGVSRQERTEPERVKSPSRGFSMGF"
     gene            4119..4517
                     /locus_tag="ACHY3M_004790"
     CDS             4119..4517
                     /locus_tag="ACHY3M_004790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319547.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQTQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQELKELKYDLDTSPTPDA"
     gene            complement(4721..5635)
                     /locus_tag="ACHY3M_004791"
     CDS             complement(4721..5635)
                     /locus_tag="ACHY3M_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4319548.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSV
                     KALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(5834..>6074)
                     /locus_tag="ACHY3M_004792"
     CDS             complement(5834..>6074)
                     /locus_tag="ACHY3M_004792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319549.1"
                     /translation="KYHKNSELEKGAKIITGLNSIFQTRKLVILTCSPFGTAEIDRAS
                     LSCIVMQRAKNDSDAFFWFSLVFPCLFAFFAPESD"
BASE COUNT         1480 a         1681 c         1326 g         1587 t
ORIGIN      
        1 aaaaaaaagc gcgtcaggat cagcctgaca cgctttgttc ttgtcaaatg aggattgctg
       61 gttactgcgt ttccactccg gtcagttcag cagttgatga cggtgataca gtcccgccct
      121 gaacatcccg gatataattt gcctgacatg cagcaagaat aatgactgca agaagcaata
      181 aaataatccc tgttattttt ttcataacga ctccttgttg tgaaagaatg ccagtcattc
      241 atccttaaac aaccggcatt catatttcag ccctgtttaa atcctgactt accgttagcg
      301 gatcgccatt ctttaatctc tttaacaatc ccttcaggac tatcttccct gtcatctcga
      361 ggataataaa tcaggtctga accatctggg tgctcagtta atcgttcaaa ctcactcacc
      421 aatttcactt gttcttcttc actagcggta tctgcattat aaattacagt aaccaactgt
      481 aaaaactcgg cctctgtata atcactgata ctatttttta tttccatctt tactgtcccc
      541 tgtgaatatc aatatgacgt ttaggcgttg tcactctgat attatccata tcatagacct
      601 cacctccctg actaatagga ttgtcgtgat gaagttcata tacctttctc cctccaacct
      661 gctgcccctt tggagtaaat ggagagtaac cctttgaaac accagattta ttactcggac
      721 ttagattttt actaagttca ggatcttttg agacttcttc ccagaatttc ttccgaaaat
      781 catcaaagct tttgaactct ttatcgcgta acttatcagc aatacgatct ggaatcggta
      841 cacctgaatc tttacccgca tcatccagcc atttatcacc aactggttta cctttacctg
      901 tcgccttccc tggcttattc cgtttactct ccttatctaa tttatcctta gcgtcctttt
      961 ctttattttc cttctgatta cggcgttcca gcgcagcacc taacgcagca tcagcatcag
     1021 acttgtcttt tgcagcagca tcaaatgcag cttgcttatt atttacatcc gtctgtgccc
     1081 gctgagcttt aagcccagcc atttgccaca ttctgtggcc gccagccatt ggatcatggg
     1141 caaatcgctc aaattgtttt atttcagcta ttgcatcagc aaaggtttta tttgccgcat
     1201 caagttcact tttacgagaa ttatagacct gctcagcctg agcctgtcgt tcctgatttc
     1261 tggcaacatc ttcatttgcc cgattcagtt cggcgcgagc ctgttcataa tttcgttcag
     1321 ctacttcaac cggatgcgta tcatcccatt cctgctgacg acgattttct tcatcctgac
     1381 gctgttttat ctgctcaggt gtcagaacgt ccgttaccga cacatacacc ggtttctgac
     1441 cactgtctgc cgggaagcgg attatcgcct catgagagcc accaccgaag gtaaagcctg
     1501 ccggaaaagc tgtccggtct ttatcctccg taactccgac gggaagcgag gttgatacag
     1561 gcgcatcgtt gacagtactg acagtcaggg aaggaacacc tggaaatgat gtgctaaata
     1621 cacccggagt gtttgtcggt tttgcatcca caacaggtac actcattggt atacctgaaa
     1681 caacggcaat atgctgtcgt tcgtccttca caacatctgt cacacgtttc gtcacgctga
     1741 cagttgcctg atccagtggc agagaactga cctgtgcatc tgtcaccgtt tctgccggta
     1801 atgacgtcac gatttttgac atcatgttcg ggtcatcttt tgctatttca gcaggaagaa
     1861 tgccgtaaag cgcaataccc caggcactga atttaaacgg gcctttcagg gctgcaaaga
     1921 tatccgctat tgccgcagat aaagcctcac cagatacgga aataccaaga gttccggcac
     1981 caggagcagc caaagcagga aaaccaaatg ccataggtgc tgctacggat gaattgctgc
     2041 caccaccgga attactattc cccccaccat tgccatggcc ggagccacca ccccagtgaa
     2101 caccactacc ggaaccgcca ccccatgggt tattttccga actccatcca gaaccgtcag
     2161 aagcaccgcc atttccacca agccccgtag ggccgccatt aatgttacca cctgtgttat
     2221 gtgcgccact gttgtggtca cgtccatctc caccgctcat aaaaactccc tcttttaagc
     2281 gttaaaacaa ttgattacat agttaatact gtacataaaa ccactggttt tatgtacaga
     2341 tcaaaaagta ctcgccatta ttttttctgt caagtcaggg agggatattt tttggcagtt
     2401 cggaggagtc actcatgaaa acggtgaacg gaacgcgaaa tcctttcgcg ggtcgtgagg
     2461 cgttggacaa gcaaagcgcg tcagtttttc cggctcttac ccgtgacagg tttctccgcc
     2521 agtttatctt ccagtgtcac aactttctga tacaccgccg ctgtggcgtt gtagttcctg
     2581 tcgagcattg ttttcattcc ccatccgagc acacagaaca taaccagaaa agccactgac
     2641 acccctccca gccatttcca gctataccct ttaagggtgt tgtattcctg cacgatattg
     2701 ctgtgaattg tcgcaatatc cctgttcagg gcgtccgcgt ttgccttcat gtttgcaact
     2761 gactcttcca gccgctgaaa atcctggctg atgcgctcac ctatcagccc tgctacgtcc
     2821 agcttctgca cctcgtttct gacttcatcc ctgacctgtt ttgccacacc gtcagaaata
     2881 tcccgatatc cgcgaagtgt ttcccttgcc acagccagag aatcagcaac agctttctgt
     2941 tgctccattg cggtatgttg cagatgctcc accgataaca ggagcgtgag taatttttct
     3001 tctgtgctgg ccatgttcag aatcccatag aaaatccacg tgacggtgat ttaacccgtt
     3061 caggttcagt tcgctcctgt ctgctcactc cgtctctcat gtcaaagcca tgctcccgcg
     3121 caacatcatg ttgcagcctt ctgaccagtt catgttgctt aaccggagtg tccagccatt
     3181 ccctgatgtc atgcccgacc gttcgcgcct gttcaagatg gcgggaatag tccggttcag
     3241 accgtttttg catgatgtca tgacgctggg catcatcaag ccgtccggca gcttcaaaac
     3301 ccagatgtac ttcgggacgt cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga
     3361 agcccctttc aaagccatgc tttctcatat gttcgttgtg cttcacctcc cagcgttttc
     3421 gcaattcccg caactgctct ttttgctgag tgagagtcaa gcttccggaa tcctttttcg
     3481 caccaccccg gtcgggatac ctggcgttat agcgccggaa atactgttcc ggtgtacggt
     3541 caataccatc attcacacgc tggctcatca tgatatgcgc atgaggctgt tcgccaccgt
     3601 caatactcgc tttcggattg tgaatggaaa aactccaggc gtgccgttcg ccggcttcct
     3661 gacgtacaaa atcctgcacc agttcaaggc gctgttccgg tgtcagttca cggggcagcg
     3721 cgatttccaa ttcccgataa gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc
     3781 agaaatggga gggattatct cgcgcccatt ccggcatgtt cccgtgctcc gtgtactcca
     3841 ggtctttccg tttcgcatac tcccctttgc gctcgatgta atcggcgtgg tttgcagcct
     3901 gtcccttgcc tccaaatttg acgctgaggt gataggttgc cacggtttca gccctccgca
     3961 ggagcgcact gtttcttgac gaaggagaga aacatataag tgcgcttcct tctaatttta
     4021 cgaattagca cggaagatac cccctgcacc caccgaccgc aagcgatata ctgcacgacc
     4081 acaacacacc gcaaaaaaca ccagggagca caacgaacat gcgcatcagc aacatcgaat
     4141 ggctgaagaa acggatcgga ttcatcagga agctcggaga acagaccgca cgacagagac
     4201 agattattga cctgctcgac aacgaagcag gactcacaga acaggaacgc aaactcctcc
     4261 acgtactggc aacagcggaa aaaaacgact tacagacaca ggagagcgaa cgaaaacagg
     4321 cggtccaaaa acgaatcgag ggaaaaaaac aacgacgaga acgcaatcac cggctctttc
     4381 tcgcggctgg gctactgata gaagcaggac tggtagacac aaaaacaggc gagctatgct
     4441 ataaaaaaga caggatatta caggaactta aggaacttaa gtacgatctg gatacatccc
     4501 caaccccaga cgcgtaagtg cctccatccg tgttccgatt ccctggtgct tcctcgctca
     4561 ttcgctcgct acgctcggtc atgagactgc ggcgagcact accagcgcaa tcatgcggaa
     4621 aacagcacca ttgcagggct gagcggggga aaacggcaaa aaacctgctg ctcaaaaaga
     4681 cgccaaaaag gcgctgttat ctgatatagg cttatttggt ctactctacg agacctcgtt
     4741 ttttcagttg gtaataccag gcgcgactga tccctaatgc ctcccacggt ttcagcgtgc
     4801 gtgcagatgt agccactgtg ctgcgcttag acttagaacc gcccttgcga ccgcgtgcag
     4861 cctgaatttc tggtgtgtgc gtatcagcca catactgtgc gaaagtttcc ggcgtgaagt
     4921 tcctgtgcgt gtatttcgca atgctcctgc caatagcccg acattcaggc ggtgaaagcg
     4981 gaacgggaag cgatgcgttg tacatttcga cacgctgaat cacggcatca agccactgtg
     5041 agaattcagg ccagccctga cgaatcgccc tgtaagccca tttgcgcgtc atttcgaaca
     5101 ggtggcagtt tcgccccatc ccgtaatgtt tatcgatgct acggcgcgct gaggcgctca
     5161 aatcgagata atcagccagt tcatcgaggg tataggcttc ctctcgccac tccatcacct
     5221 gccattccag gtggaacgga tttttacaaa tcagtccgct gtagttcaca tcagcacaaa
     5281 gtttctcaca cagcgaacgt tcaatcgcag cggcgtattt cagcgcctta accgagctgt
     5341 ccggcgcggt tctcacggcg atattgagcg cgtagagcag gtgagcatga ccgttcacag
     5401 gattttttac ggtgatgttc ggcgcgggag cgttccggtc gctccagtca atcgctgccc
     5461 cctcgcggtc aacgtcaaaa accagccaga actgcgcatg aggctggttc tgctgtatgt
     5521 accgcgcaag aagcgcacgc cctttgccgg agatgcgaag accaaaagcg agatcatccg
     5581 tgtgataggg acggtggggt aaattttcgt cgaagtattg aagcgcggcg ctcatgtgtt
     5641 acgattctct ccgggttatg cgacctacac gccctctgta tttcatgttg aatcttgccg
     5701 gatctggaca tgatttcagg ggcgtttgtt tttcagtcgc ttacactaca cccaacggca
     5761 aatcctgtcg aaccttcccg aaaccccgat ccatttacca taactcccgt tgtacgcccc
     5821 ccttaaaacg ccctcagtcg ctctctggcg caaaaaacgc aaaaagacaa ggaaacacca
     5881 ggctaaacca gaaaaatgcg tcagaatcgt ttttagcgcg ttgcataact atgcacgaaa
     5941 gcgatgcgcg atcgatttcg gctgttccga agggcgaaca agtcaaaatg acaagttttc
     6001 tcgtctggaa tatggagttc aacccggtga tgattttagc gcccttctcc agctcagaat
     6061 ttttgtgata cttc
//
LOCUS       DAZUFB010000186         6000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZUFB010000186 DAZUFB010000000
VERSION     DAZUFB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.186"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..84
                     /locus_tag="ACHY3M_004793"
     CDS             4..84
                     /locus_tag="ACHY3M_004793"
                     /inference="COORDINATES: protein motif:HMM:NF022024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319550.1"
                     /translation="MKKPQLLARIERLEQHGQTVIPGLTN"
     gene            312..554
                     /locus_tag="ACHY3M_004794"
     CDS             312..554
                     /locus_tag="ACHY3M_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFS4319551.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            634..1059
                     /locus_tag="ACHY3M_004795"
     CDS             634..1059
                     /locus_tag="ACHY3M_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFS4319552.1"
                     /translation="MATEKKNVGRPSDYLPEVADDICALLASGESLVKVCKRPGMPAK
                     ATVFRWLSEHEEFRDKYAKATEARADSIFEEIFEIADTAIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            1056..2471
                     /locus_tag="ACHY3M_004796"
     CDS             1056..2471
                     /locus_tag="ACHY3M_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFS4319553.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            2473..4671
                     /locus_tag="ACHY3M_004797"
     CDS             2473..4671
                     /locus_tag="ACHY3M_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4319554.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEDQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLKN
                     KNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQASIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKPTQQQPQQ"
     gene            4762..5655
                     /locus_tag="ACHY3M_004798"
     CDS             4762..5655
                     /locus_tag="ACHY3M_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFS4319555.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFNEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLGKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            5674..>6000
                     /locus_tag="ACHY3M_004799"
     CDS             5674..>6000
                     /locus_tag="ACHY3M_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFS4319556.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAG"
BASE COUNT         1694 a         1421 c         1606 g         1279 t
ORIGIN      
        1 aaaattaaga agcctcagct actggcgaga attgaacgcc ttgaacagca cgggcaaacc
       61 gtaatccccg gactcaccaa ttaacggcag tacagcgaaa caacccaagc cagtaagtgg
      121 ggaaataaca ctggcagcca ctgaaagatg aacctccagc cttatggcaa aaaagattct
      181 ttgtggtggc ggactgatgg aaagacatcg gttattgcag aggccattca atgagtggtc
      241 tcgacaatgg cttataccct acacgggata acttaactga tatccctttt aacggataaa
      301 cggagccaac aatggcagag attattccca tgactgaaga acagaaattc cagttagaga
      361 tttacaaact ggtcatgaac cagaacgcag ccgcagaaga agcatttcag ttcattggca
      421 ctgacgagct gaagcttgag ttattcaaaa ttcacttcca gtcaggcggc gctaattcgg
      481 atatcacgac ccgcactatc gaagcggtgc gtaaatcgaa ggaagcttta gacctgttca
      541 ccaccggagc ataaacatgg caactcaagg tttcgacaac ccatccaaat tccgcgatga
      601 atgggataag caagcagaag ggaaataatc aatatggcga ctgagaaaaa gaatgtcggt
      661 cgcccttcgg attacctgcc ggaggtggct gatgatatct gtgcgctgct tgcctccggg
      721 gaaagtctgg ttaaggtttg caagcgcccc ggcatgccag caaaggctac tgtatttcgc
      781 tggctgtcag agcatgaaga atttagagac aagtacgcga aggcaactga ggcacgagct
      841 gattctattt tcgaagagat attcgaaatt gctgacactg cgattccaga tgctgctgag
      901 gtggcaaagg caagacttcg cgttgatacc cgcaaatggg cgctggccag aatgaatccc
      961 cgtaagtatg gcgacaaggt aactaatgag cttgtcggca aagacggcgg cgcaatccag
     1021 attgaaacat caccgatgag cactctgttc ggaaaatgac ctcgattaat cctatctttg
     1081 aaccgttcat tgaggcgcat cgctacaaag tcgccaaagg cggtcgaggt agcggtaagt
     1141 catgggcaat tgctaggctg cttgttgaag cggcgcgtcg tcagccagtg cgtattctct
     1201 gcgctcgtga actgcaaaac agtatcagcg attcggtaat ccggttgctt gaagatacca
     1261 tcgagcgtga agggtattcg gccgagtttg aaattcagcg ttcaatgatt cgtcatctcg
     1321 gaacgaatgc tgaattcatg ttctacggca taaaaaacaa cccgactaag attaaatcgc
     1381 tcgaaggcat tgatatctgc tgggtggaag aagcggaagc ggtaacgaag gaatcatggg
     1441 atatcctgat accgaccatc cgtaagccgt tctctgaaat atgggtgagc tttaacccga
     1501 aaaacatcct cgacgatacc tatcagcgat tcgttgtaaa tcctcccgat gatatttgcc
     1561 tgctgacggt gaactacacc gacaacccgc actttcctga agttctccgt ctggagatgg
     1621 aagagtgcaa acgcagaaat ccgacactgt atcgtcacat ctggcttggt gagccagtga
     1681 gcgcaagtga tatggcaatc atcaaacgtg aatggcttga agccgcaacc gatgcgcaca
     1741 agaaactcgg atggaaggcg aaaggcgctg ttgtctctgc gcatgacccg tcagatacag
     1801 gaccggatgc taaaggttat gcatcgcgtc acggttcggt ggttaagcgc attgccgaag
     1861 gtctgctgat ggacatcaac gagggtgctg actgggctac ttcgctggcg attgaagacg
     1921 gcgctgatca ttacctgtgg gatggcgatg gtgtcggtgc agggctacgc agacagacaa
     1981 cggaagcgtt ctccggcaag aaaatcaccg ccacgatgtt caagggcagc gaatcgccat
     2041 tcgatgaaga cgcgccgtat caggccggag catgggctga tgaagtcgta cagggtgaca
     2101 acgttcgcac tattggcgat gttttccgca ataagcgagc gcaattctat tacgcactgg
     2161 ctgataggct gtatctgaca tatcgggcgg ttgttcacgg tgagtatgca gaccccgacg
     2221 acatgctgag tttcgacaaa gaagcgatag gcgagaagat gctggagaag ctgtttgcag
     2281 aactgacgca gattcagcgc aaattcaata acaacgggaa gcttgagcta atgactaagg
     2341 tcgaaatgaa gcagaagctc ggtattccat ctcctaacct ggctgatgcg ctgatgatgt
     2401 gtatgcattg cccggagtcg gctgcgcaac ccgactattc cagttactca attccttgtg
     2461 gtgtaggttg atatggcaga aaaaaagatg actgactggc atcgcaaggt gctgtgcaac
     2521 tttgataatg cctggtcagc aacgcaggat atgcgtgagc agattattga ggctcaacgt
     2581 ttcgtccggg tatccggcgc acagtgggaa ggcagcacaa acgctggtta ctcatttgat
     2641 gaaggcaggt ttgagcatta cccgcgcttt gaactgaata agattgcccg tgaatgtgat
     2701 cgcatcattg gcgagtatcg acagaatcgc atcagcgtta aattcaggcc gaaggacgat
     2761 aaggcatcgg aagcgttagc cgaaaagatg aatggtaaat tccgcgctga ctatcaggaa
     2821 acatccggtg gtgaagcgtg tgataacgca tttgatgatg ctgtaacggg cggattcggt
     2881 tgtttccgca tgtgtgccga ttacgaagat gaaatggatc cgagtaacga gcaacgccgt
     2941 ataagccttc tccctgttta cgacccagcg acatgcgtct tcttcgatca ggacagcaag
     3001 caatatgacc gctctgatgc tatgtgggct atggaaatgt tctccatgac gcctaaagcg
     3061 ttcgaagctg aataccctga ttccatcgcg gcaagccttt ctcgtgatga cactggtact
     3121 cagtatgact ggtcaacgcc cgatgccatc tatgttggac gctactacga agttcgcata
     3181 gagaaggtga agctcacagc atggcgtaac cctgttagcg gagaaacggc aatctatgat
     3241 gaagatcaaa tcaaagatat tgtcgacgag ctgaccgatg gcgcattcga actgattggt
     3301 gagcggacag tgaagaaacg ccgagtttat tgcggtcttc tgtctggcgc tgaatggctg
     3361 gaagaaccga agcgtattcc gggcgaacat attcctctca tcccggtata tgggcgtcgc
     3421 tcatttgttg ataatcagga gcgaatcgaa ggtcacgcag caaaagcgat ggatgcacag
     3481 cgtcttgaga acctgatggt ttccatgatt gcagataacg ctactcaggc tggcggtgat
     3541 ggcattcctg tagttgatgt tgacatgatt cctggtcctc tcgccactca ttgggcggag
     3601 cgcaacaaaa agcgcccggc gttcctgccg atggtcagtc tgaaaaacaa aaacggagat
     3661 attactgcgc aggctcaggt cagcagttat acacctccga cacaaatgcc tcctgctctt
     3721 gccgggctat tgcagtacac cggaacggct attcagcaaa ttacaggtgc gtcgcagctt
     3781 gagaacatgc cgagcaacgt cgccaccgat accgttgata gcatctttaa ccggatggac
     3841 acgcagtcct atatctacat ggacaatatg gctaaatcca tgcgccgcgc tggcgtcgtg
     3901 tggctttcta tggctcgtga agtctatggc agtgatacgc cgatgcgtat cgttaatgag
     3961 gacggcagcg atgacgtggc gctgatgact ggtgaagtgg ttgaccgtca gacagggcag
     4021 gttatcgcgc ttaacgacct ttcgcagggt aactatgaag tgactgtcga tgtcggtcag
     4081 tcgttcgcta ctcgccgtga cgcaacggtt aagtcgttac tttccatgct ggcacttatc
     4141 ccaccaggaa cgccgaagca cgaccttgta tcgtcgatga ttctcgacaa tatggacggc
     4201 gaagggatgg acgaccttaa agaatacaac cgcaatcagt tgcttctgtc tggagttatc
     4261 aagccgagaa cgccagaaga gcagcaaatg gttgagcagg cgaaacaaca acaggccagt
     4321 cagccagatc cggctatggt tgctgcgcaa ggtcagcttc ttgctggtca ggctgaattg
     4381 cagaaagcgc agaacgaaca ggcatccatt caggttaaag cattccaggc acagactgat
     4441 gctcaggttg cagcggcaaa tgttgtgaaa atcctcgcat ctgccgatag ccagcagaaa
     4501 tctgatatcc gcgaggctct gaaactgcta ggacagttcc agcaacagca aggagacaat
     4561 gcccgtgctg atgcagagct tgtcctgaaa agtcaggcac agggccatgc gcagcgcatg
     4621 gacatcagca gtatcctgca aaaaccaact cagcaacaac cacagcagta attaacccat
     4681 aacgtgcaat ggctgtcttt atgaggcctg gcaccctatt gccttccgat gggctgaaca
     4741 tcgagtaaac aggggtaaca aatggaccag atggcagaaa acacaccaga agttgaaatc
     4801 gaaaccgacg cgtcagagca gattcctgat gatgtcgaac tggctgaaga agtcgaaaca
     4861 gaagatggca gtgagtcctc cggcaatgat gcagaggaag ctactgaaac tgatgacgac
     4921 gaatcagaac aggaattcta ctttggtgac gaaaagctgg attcgccaac cagcgaagat
     4981 ggcgcagagc atggactggt aaaacacctg cgcaagacga ttaaagagaa agaccgcgag
     5041 ctgaaagagc tgatgcgtca gtctcagaaa cccgtcgagc agcagccggt aatcactcaa
     5101 ccaccgcgaa tgccaaaact ggatgatgag gacatcggtt tcaatgaaga aatctaccag
     5161 caacgcatgg ctaagtgggc agaggataac ggcaagtacc agcaacagga gatggctcgc
     5221 aagcagaagg agcaggagct tcaggctgcc tatcaagagc gattatccaa atatcagcaa
     5281 cgtgttaagg ctctcaaagt tcctggctat caggaagcag aacaggccgt actcgaggaa
     5341 atccccatcg agacacaaaa cgcgatcctg tttgagtcag agaagccgga aatcgttgtt
     5401 ctggcactcg gtcgcaacgc tgaactgcgc aagcaactgg cagaagctac caaccccgta
     5461 gcaattggtc gtctgctgga acgtatcgaa tcgaaggcca gaatcatgcc aaaagcaaaa
     5521 accacggcag ccacaacccc gacagttaag gggagcaacg gcgcagtaat caacaacctc
     5581 ggcaaattga aagccaaggc gctggaaact ggtgactgga cgccgtattt cgccgctaaa
     5641 aaggcaaaaa aataacctat cggagcatta agcatggcta accaattagc aaaagacctt
     5701 gaaatcatgt tcgaaaacta cgttgaaggc tttgaggccg cctgcgtagt ttcccgtaac
     5761 gctaaaaaat tccgtcccgg tgatacagca atgcagcgag caggtgatgt tctgtatcgt
     5821 ccgcagcatt accacatgaa tattgaggaa ggcctagacc tcagcggcaa aacgccaaca
     5881 gcactggttc agcgccttgt tccttctgtg ttcaaggagc cgaaaaacat tctgtacact
     5941 ctggatgcgc gtgaaatgcg tgacccggaa cataaaactg aagctggtcg agccgcaggt
//
LOCUS       DAZUFB010000187         5953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZUFB010000187 DAZUFB010000000
VERSION     DAZUFB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.187"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1036
                     /locus_tag="ACHY3M_004800"
     CDS             <1..1036
                     /locus_tag="ACHY3M_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFS4319557.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     PVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQG
                     PTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTIN
                     EMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKN
                     IEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGG
                     MNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYN
                     EMVDDYNSLR"
     gene            complement(1086..2834)
                     /locus_tag="ACHY3M_004801"
     CDS             complement(1086..2834)
                     /locus_tag="ACHY3M_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFS4319558.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2834..3904)
                     /locus_tag="ACHY3M_004802"
     CDS             complement(2834..3904)
                     /locus_tag="ACHY3M_004802"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4319559.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            complement(3894..5345)
                     /locus_tag="ACHY3M_004803"
     CDS             complement(3894..5345)
                     /locus_tag="ACHY3M_004803"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFS4319560.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIIGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5356..5802)
                     /locus_tag="ACHY3M_004804"
     CDS             complement(5356..5802)
                     /locus_tag="ACHY3M_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFS4319561.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
BASE COUNT         1546 a         1508 c         1517 g         1382 t
ORIGIN      
        1 aaggaattta ttatcttccg ctattatagt cgccatcatg gccctcggtc tgacgggttg
       61 tgatgataaa aaagccgaaa cagaaacgcc cccgcctgcc aatagtcaac ctgccgcacc
      121 agctcctgaa gctcccgttg caaaagcaga agctaaacct gaaacacctg cgcaaccggt
      181 ggtcgatgaa caagcggttt tcgacgaaaa aatggatgtc tatatcaagt gctataacaa
      241 gttacagatc cctgtacaga gcagtctggc gcgttatgct gactggctga aagattttaa
      301 acaggggcct accggtgaag agcgtactgt ttatggcatc tacggcatta gtgaatccaa
      361 cctcgctgag tgtgaaaaag gcgtaaaaag tgctgtggcg ttaacgcctg cgctgcaacc
      421 aattgatggc gtggcggtga gttatatcga tgctgccgtg gcgttgggta ataccattaa
      481 cgaaatggat aaatattaca cccaggaaaa ttataaagac gatgcgtttg cgaaagggaa
      541 aacgctgcac cagacattct taaaaaatct ggaagccttt gaacctgtag cagaatctta
      601 tcatgcggcg attcaggaaa ttaacgataa gcgccagctt gcggaactga aaaatattga
      661 agaaagagaa gggaaaacat tccactacta ctctctggca gtcatgattt cagcgaaaca
      721 aattaataac ctgatatcgc aagagaagtt tgatgttgat gcggcaatga agaaagtgtc
      781 tgaactggaa acgctagtgg cgcaggccaa agaagcggat aaaggcggca tgaatttctc
      841 gtttattaat tcggcaggcc agtatcaact cgaggctaaa aaatacgttc gccgcatcag
      901 agataaagtc ccgtactctg actgggataa agagcaactt caggacgcaa actcaagctg
      961 gatggtcgaa gactctttcc cgagagcatt acgcgagtac aacgaaatgg ttgatgacta
     1021 taatagcctg cgttaaacgt ttttgatgat aaatgcagtg tcggatgcgg cgcatccgac
     1081 acagttcacc ccttgtgaag taactccata aatgcctcat atcctgttaa cccggcaata
     1141 atctccgccg gatgctgaca caatatgctt gccagatagc taaatatttt tagctcatga
     1201 cgcgcatcgg cggcggcgca ggcgatcaag acatggttaa tgacttcgtt attcacctca
     1261 actggttggg cgagggtaat aaaaaatcca cgaaaacgtc gctcctgttc gctccagcaa
     1321 tgtgggatgg cgaggcgatt aacaatcagg ttttcacctt cgccttcgcg ggcgatgatg
     1381 cgttgtgctt catcggcggt aatatggtgt tgtgcgacta attgcgcaca aatttgccgg
     1441 gtaatatgtt gccagctttc accgcgtaca ttcgaataat gaaaacttcc gggagcagag
     1501 aaaaaacgct ccggctgttg gcgaataaat gccgtcgcca gaaaatgttt tatttgttcg
     1561 ataccggcag ccgtaatgat attttttacg gtgatgtaat tatttaccgc gtcatccagt
     1621 aaataatggc tgttgttaat gatcaataac ggctcaatct cttcgcgaat ggcaacaagt
     1681 tcgcttaagc tacgggcaat aatcacccga caatgtaaaa catcgcgctc aatggcgagc
     1741 tgattaatag tggcaatcgc gttctggtcc gagagcaaaa tgatcggctg gcgttcgttt
     1801 tgatgccgtt ccagcgcaca ggcaaaatac aaaccgatca gatcgctgtc gaaaagggga
     1861 atatcgagct gttcacgcag tagcgtaata aagtgcagac tcatatcaaa ggccgcaggc
     1921 caggcggctt tcaggttatt catgctgctt tggcggtgct cagcaatcca caccggtgcg
     1981 gcaaggcaac gtaataaatg cccggtaatg ttctcaacca gctgcgtatc aataatattc
     2041 aatgacgcga tgcccggcac cctttgcagc aaccgctgca cattatcggc agaaattatc
     2101 tgcgcttgct gatgctgttt ctctatcaaa cctgtcaggg ttcttacagc gttatcacca
     2161 agaaacagac cgctatgttc aacgatttgt tttatctcgt caccgggata ttgcggccac
     2221 tcatcggtca gttgattacg cagggcgtaa atcgccagaa tcaggctgga gagataatca
     2281 ccctgcatga gcggccagcg gtgttggttg tcgcaggccg tggataaatg ctgaaggttg
     2341 tctcgtgtta cgcccgccag cggaattaaa aacggatctt tgcgcagcaa gttcgccagc
     2401 aatataacgc gtttttcttc tgtctcatca atgaaatggc ccaaaccggg gcgactggcc
     2461 aggcagcaag tgcgttcata acgctgtttt aaccggggca gacgctctgc gacccacgtt
     2521 tccggcaaat taagcgccga ggcgagttgc gcacggggag taaaggtatt cagtaataac
     2581 agtgccagca gccgatcgtc gttatcgtgc ttttgcagca actgaaaaaa gctgcggcgc
     2641 tcgaagattt ccagctgata gcccgcacta ccactggcag aaatgcgggc tttgccgtta
     2701 agggtaaagt tgagatagtc aatatcacgc agtatggtcc tgccagaaac gccagtctgt
     2761 tgcgccagct cgccaggcgt gcgcggctgt tgctccagca gatcgacaat ctttaactgg
     2821 cgttcgttta gcattaatcc acctgacgga actggctaaa cgcgctgact ttttccgcgg
     2881 ttaaggtggt cagaaaaccc cgtatcagcg tgagcagagc atcgtaatcg gcttttgaaa
     2941 tcatgccgct gttggcgtgc agataacgcg ttggcaaaca cagcgcgaca accggacgcc
     3001 caccgcccat cacgttgtag cgcccgccat ccgtcgcacc ggttttcatg gtggaaaatt
     3061 gcagcggtaa atcattctgc gtggcacagc tttttaacgc tgctaccagt ttctggttgg
     3121 ggaagtagcg cttgtcaaac agcatcaacc ccggcccgtt gcccagtttc agcggatatt
     3181 taatgttatc aatgcctgga acatcgcccg cgacagcggt gtccagcacg atcaccacat
     3241 ccggtttaat gtgttcagcc gaggtttgcg ccccgcgtag cccaacttct tcttccacac
     3301 tgccaacgcc atacagcgta atttcgggat tattcaccgt ctgtagtagc tcagccatca
     3361 ttgcgcagcc gatgcggtta tccaacgcct tgccgaccac tttatcttcg ccccagcagg
     3421 caaaattggc ttccgggcta ataaaatcgc ccatcgccac accgcgtttt tccacctcat
     3481 cgcgactgtt tgcgccaata tcaatgaaca tctcatcaaa tgactgcggc tgttgctttt
     3541 gcttttccgt taacgcatga ggcgcgacgg aaccaatcac accagggatt ttcactccct
     3601 tgtgtgtgcg tatggttacc cggtggttga gcatcgactg attccaccag ccaccaatgg
     3661 tggtgaaacg cagaaaaccg ctctcgtcga tgtgggtgac cataaagccg acttcatcca
     3721 tatgcccgac aacggcaact ttcggccctt tatttccctt acgggcgaca aagctgccca
     3781 gcccatcaaa ggtgatttca ttcacgcaag gttccagcgt gtttatcaga atgtcgcgaa
     3841 cttcctgttc atcgccgcta acggcgctgg cttcgcacaa ctgttgcagt aactcaatgt
     3901 tcatggacaa gctcctgttg cgcggctttc gctttgcggc gtaaccacaa tcctttcagg
     3961 acgataatga atgaaatgtt cagcgcgagg ccaatcgcca gcaccagata aaacgaacct
     4021 accggtgaca tcaggccgat cagcgggtca aagataccca aaccaggtgc cagacgtttg
     4081 attccgaaag cgatcaccag cacgccggta ataccgcccg ccagcgtatt ggcggtaatc
     4141 atcggcaacg gagctgccag agcgtaagga atagccggtt cggttgcgac tgtggcaccg
     4201 accacaatgg cactactggc agcctctttt tcctgtcggg taaacagttt gggcgcaatg
     4261 aacgtcgcaa gacccgccgc gacgggcggc atcagcgcga caacgccgat gatggcgtac
     4321 cagtcataaa tgtgtttttc cagcagtgag aagcagaaga accaggcggt tttattgatg
     4381 gggccgccca tatcgaacgc cagcatcgca ccgaccagaa acgctgccgc aaacttcatt
     4441 gatggaggaa tggtattcag gaagtggagc agcccgccca tgaggtcgga catcaccggc
     4501 ccgataacgt agtaagtgag cacgccaaaa accagcagag taacgaatgg gataagcatg
     4561 gagccgagca gcggttgtaa tgctttgcca agacggactt tacggaacca gaagacgaaa
     4621 tagccaatcg ccagccccag caccaccgcg ccgaggaagc ccgcgcctga ctgggtgccg
     4681 agcagggctt tatcgttcgc cagatagcaa accagaaatg ccggagcgaa agcgggttta
     4741 tcggcaatcg acgaggcaat ataagcgccc atgatgggga tcataaaggt gaagccgagg
     4801 taaccgatgg attcaactac ccaggtaaac gacggcgcac ctttcgacat atcggtatat
     4861 ggcaaaccga actgcaccag catgttggca agcgccacca gaataccgcc gccgatgaca
     4921 aatggcagcg ccgcagagac gcccgccatc aggtggctca tcacgctgcc gctttgcatt
     4981 tcctgtttgc cgagcttcac gccgctatct gcggcaaaaa gctgcgagtt tgttggtaat
     5041 tcgctgaaaa tctggtcgat atttttcagc gcctgggaga tggcaatctc ataaactttc
     5101 ttcccggcaa accgcgcccg gtcatcaccg ctcaggccac gcccggtagc tagaatgacg
     5161 taatcggcag cggcaatctc ttcgctggat aagcggtttt caacgccgct ggacccttga
     5221 gtttccactt ttatggtatg gccgagagaa cgggctttct gttccagggc ttccgccacc
     5281 atgtaggtgt gagcgatccc ggcggggcag ttggtgatcg cgacaatacg taaggatgac
     5341 tccatcgcca cttccttatg agctgagggt ttgatttaac aaagcaagta cctggtcggc
     5401 gtcgccctgt cgcagttggt tgataaaatc ctgatggata attttgcgac acagtgtgcc
     5461 gatgattttg acctgatcct cttcgccact ttgcggcacg ccgaggcaga tccagcaatt
     5521 gacgtcttcg ccgtcgctgg ctttccagtc aatggcctgc gctttgcggg cgaataacac
     5581 gaacggttgt ttaacgcagg cgcttttacc gtgcggcacg gcgatgccgg aaccaaatcc
     5641 agtagagtgc attttttcgc gcagcagcag ggtttgcagg aactggtgtg agtcggtgat
     5701 aaaaccgttt tgtaacgcca ttgtcgccaa ctgcttcaga atggaataag cgccattgcc
     5761 ctgaatattc aggtcaatac agcttgcagt aagagctgcc attaatcgga ctccataaaa
     5821 tatattttaa tcaattgtaa tggtggagcg attaagtgat gagaggtgat atgtcggtaa
     5881 acaatgacaa tttgagacag agtgaaaggc cagatttgcg gagtaatgca cataatggtt
     5941 atttaaataa acc
//
LOCUS       DAZUFB010000188         5916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZUFB010000188 DAZUFB010000000
VERSION     DAZUFB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.188"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..1232
                     /gene="yiaY"
                     /locus_tag="ACHY3M_004805"
     CDS             81..1232
                     /gene="yiaY"
                     /locus_tag="ACHY3M_004805"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFS4319562.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1397..2935
                     /gene="aldB"
                     /locus_tag="ACHY3M_004806"
     CDS             1397..2935
                     /gene="aldB"
                     /locus_tag="ACHY3M_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFS4319563.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3154..3357)
                     /locus_tag="ACHY3M_004807"
     CDS             complement(3154..3357)
                     /locus_tag="ACHY3M_004807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319564.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3480..3803
                     /locus_tag="ACHY3M_004808"
     CDS             3480..3803
                     /locus_tag="ACHY3M_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS4319565.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3809..4945
                     /locus_tag="ACHY3M_004809"
     CDS             3809..4945
                     /locus_tag="ACHY3M_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4319566.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKGTRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYLSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4942..>5916)
                     /locus_tag="ACHY3M_004810"
     CDS             complement(4942..>5916)
                     /locus_tag="ACHY3M_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4319567.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1460 a         1412 c         1545 g         1499 t
ORIGIN      
        1 aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc cacccttttt
       61 tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct gtgaatgtca
      121 tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga tttacccgta
      181 ccttaattgt cactgacagt atgttaacga aattaggtat ggcgggtgat gtgcaaaaag
      241 cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct aaccccacca
      301 cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat agcgtgatct
      361 ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg gcagccaatg
      421 gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag ctgccgatga
      481 tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc atcatcactg
      541 acgaagcgcg tcatatcaaa atggcgattg ttgataaaca tgtcactccg ctgctttctg
      601 tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca acgggtatgg
      661 atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg atcactgacg
      721 cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc gttgaagatg
      781 gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttcctcgcc ggtatggcgt
      841 tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc ggtttctaca
      901 acctgccaca cggtgtatgt aacgccgttt tgctgccgca tgttcaggta ttcaacagca
      961 aagtcgccgc cgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac gtgacaggta
     1021 aaaacgatgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg gcgaagaaag
     1081 tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc gcggttctgg
     1141 cgactaatgc cctgaaagat gcctgtggtt ttactaaccc gatccaggca actcacgaag
     1201 aaattgtggc gatttatcgc gcagcgatgt aatcatcatt tccacaacgg ctggcaaatt
     1261 gtcagccgct ttttcaacta tctctgtaac ccttgcccgt aaattcgtga tagctgtcgt
     1321 aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt atacctcaca
     1381 ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc ccggcgagta
     1441 tggtttcccc ctcaagttaa aagcccgcta tgacaacttt attggcggcg aatgggtagc
     1501 ccctgccgac ggcgagtatt accagaatct gacgccggtg accgggcagc tgctgtgcga
     1561 agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc acaaagtgaa
     1621 agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta agattgccga
     1681 tcgaatggaa caaaaccttg agctgttagc gacggctgaa acctgggata acggcaaacc
     1741 cattcgcgaa accagtgctg ccgacgtgcc gctggcgatt gaccattttc gctatttcgc
     1801 ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa ccgtggccta
     1861 tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact tcccgctgct
     1921 gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg tgctgaaacc
     1981 cgcacgtctt accccgcttt ctgtactgct gctaatggaa atcgtcggtg atttactgcc
     2041 gccgggcgtg gtgaacgtgg tcaacggcgc aggtggggaa attggcgaat atctggcgac
     2101 ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc aacaaattat
     2161 gcaatacgcc acgcaaaaca ttattccggt gacgctggag ctgggcggca aatcgccaaa
     2221 tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag cgctggaagg
     2281 ctttgcactg tttgccttta accagggtga agtttgcacc tgtccgagtc gtgccttagt
     2341 gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg aaagcattcg
     2401 cagcggtaac ccactcgaca gcgtgacgca aatgggcgcg caggtttctc acgggcaact
     2461 ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgcagacg tgctcaccgg
     2521 cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg aaccgacgat
     2581 tctgtttggt cagaacaata tgcgggtgtt ccaggaggag atttttggcc cggtgctggc
     2641 ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata cgcaatatgg
     2701 cctgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg ggcgcggcat
     2761 acaggctggg cgcgtgtgga ccaactgcta tcacgcttac ccggcacatg ctgcgtttgg
     2821 tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg agcattacca
     2881 gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggctgt tctgatataa
     2941 gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct tatcatgcct
     3001 acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat aaacaaagcg
     3061 cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact acgccaacag
     3121 aagtgcgttt ctggggcgtg ttttgccagg agtttactct cgcgtttcga tggtgagcgg
     3181 gcaaaccagg ccgtaaatca ccattggcgt tccgaggcca atcagcaata aacggtagat
     3241 aattgaaggt ttaatacgct ttatctgtac tgtggatgtt ttactggagt tattcattag
     3301 cttggtcaat agttccatta tttcaatatg ataaacctcg acctgttgaa aagccacgca
     3361 gcacacttca gaaagtatat aaaaaacaat atgccgttat ataaataagg aaattgcact
     3421 gcatcatggg atttcataat atgcgttaat tgttcttttt tatattctct gagggaaata
     3481 tgtttctgga ttattttgca ctgggagtgc ttatttttgt atttctggtg atattctatg
     3541 ggattattat tttacatgat attccctacc tgattgctaa aaaacgtaat catcctcatg
     3601 ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt atctggccat
     3661 ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg caaaaccatg
     3721 actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag ctcgccgaca
     3781 tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga cctatgtggc
     3841 ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga ccattcccac
     3901 agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga actataacca
     3961 tccgtatacc ttcaaagcgc aaaaagcggt tatttctatt cctgttgtcc cacaggtgac
     4021 aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag gtgaggtgct
     4081 atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg cggatatcgt
     4141 taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg cggcgaatac
     4201 tcagcaggca aagggcacgc gggataaatt cgctaaagag tataagcgtt acgcacgtgg
     4261 cagccaggcg aaagtaaacc cgttttcaga acgcgatatc gatgtggcgc ggcaaaatta
     4321 tctggcgcag gaagcctccg tgaagtcatc ggcggcggaa caaaaacaga tccagagcca
     4381 gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag cacagctcgc
     4441 ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg gttatgtgac
     4501 ccaggtactg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc cggtgatggt
     4561 gtttataccc gatcagaaac gacaaatcgt ggcgcagttc cgtcagaact ccttgctgcg
     4621 actggctcct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta aggtattcag
     4681 cggtaagctg gcagccatta gtccagccgt tcccggcgga gcttatctgt cgaccggcac
     4741 cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca ttgaactgga
     4801 tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg cggtttactc
     4861 tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga ccagctgggt
     4921 gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag gccgcagttg
     4981 ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca catactggac
     5041 ccgccagggc tggtattccc gtttacggaa gatagccacc agatccagcc accattcata
     5101 gggttgctga aagcagggca ccagcgtgcc gtcttgcagg tcgctctgca cacttttgtc
     5161 tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgcagatt gcgtgttgtc
     5221 gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg cgacgttgaa
     5281 gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg agtgatgttc
     5341 gagttcgtca ggacggctaa tgggatgttt ctcaagatag cgctggctgg cataagcggt
     5401 tacggcatat tgggtaataa aactggcaac cagcgattca tcttttggtg gagcgtagct
     5461 gattaaaaca tcacaatcat caggaaattc gacgccttca taaaaggcat tacgctcaag
     5521 attgcaggtt tttagcgata aggtgatatc accgatatct ttaattttat cgattacatg
     5581 tttggataaa taggtaatta tgccggttgg ggcgtagatg gtgacccgac cacgtttctc
     5641 atgcttataa tctgcaataa aattattaag ttgctcgttt ctgtccagca tgtcgttgat
     5701 gtacggtaac agcgcggtgc caaaaggtgt gagcatcagc tgccgggtgg ttcggtcaaa
     5761 aacttttaaa cccacttttg attcaaaatc agcaagatat ttgctgacgt tggcctgtgc
     5821 gatgccaagt acagtcgcgg catggctgat attttcactg gcagcgatta ccgagataat
     5881 ttttaactcc cggtacttaa gttgtaattt tgtcat
//
LOCUS       DAZUFB010000189         5885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZUFB010000189 DAZUFB010000000
VERSION     DAZUFB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.189"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            351..869
                     /locus_tag="ACHY3M_004811"
     CDS             351..869
                     /locus_tag="ACHY3M_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS4319568.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1079..3220
                     /locus_tag="ACHY3M_004812"
     CDS             1079..3220
                     /locus_tag="ACHY3M_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFS4319569.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3296..>5885
                     /locus_tag="ACHY3M_004813"
     CDS             3296..>5885
                     /locus_tag="ACHY3M_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS4319570.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     AYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTRKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPA
                     GQLQSQH"
BASE COUNT         1378 a         1529 c         1859 g         1119 t
ORIGIN      
        1 tgattaagtg ggtattttta aggaatatct caaaaattat caaaatgata attcagaaag
       61 gataaataca atatttattc atgattcagc tttggccatt ttatggaggc aatacggact
      121 ttgaacagtt gggtatggtt atccagatat atattattaa gttattgctg aataagagat
      181 ttaatgattt taaaaatagt ttttacgaca accgtaaatt ctagctcggt ttgtatgtaa
      241 aaaatactga aaaatgatgc ggttccgtga agtgagctgc atctgcctgt ctgggattaa
      301 cggcaggtca ttgtaagtag taattaacca cgttaacaag gagtttacaa atgccaacac
      361 cgtgttatat ctccatcact ggtcagaccc agggaaatat tactgcaggt gcatttaccg
      421 ctgattctgt cggcaacatc tatgtgcagg ggcacgaaga cgagatgctg gtgcaggaat
      481 tcctgcataa cgttactgtc ccgaccgatc ctcagtccgg tcagcctgcg ggtcagcgtg
      541 ctcacaagcc tttcattttt accgtggcgc tgaacaaagc ggttccgctg atgtacaacg
      601 cactggcctc cggcgaaatg ctgccaacca ctgaacttca ctggtggcgt acctctgtgg
      661 aaggcaaaca ggagcattat ttcactaccc gcctgactga ttcgacgatt gtcgatatga
      721 aactgcatat gccgcattgc caggacccgg cgaaacgcga gttcacccag ttgctcgaag
      781 tttcactggc ctaccgcaaa attgaatggg aacacgtcaa atctggcacc tccggtgccg
      841 acgactggcg cgcaccgctg gaagcataag ttaagccaac agcatccggc tatcaggccg
      901 gatgctgttt ttgtattacg ccgtagaaag aaaggttgtc agtaaggggt tacgttcacg
      961 ggagatggcg gctgccggaa acttcggcgg tagcgtgcct gaacgcaagc gtaacactgt
     1021 actgtcacag ccgctttaac agcgaactgt ctgtttttcc ttaaccccgg aggttgctat
     1081 gtcaaccgga ttacgtttca cgctggaagt ggacggcctg ccaccggatg cttttgcggt
     1141 ggtctccttt catctgaacc agtcactctc ttcgcttttt tccctcgatc tctctctggt
     1201 cagccagcag tttctctccc ttgaattcca gcagatcctc gacaaaatgg cctacctgac
     1261 gatatggcag ggcgatgacg tacagcgccg ggtgaaaggt atggtgacct ggtttgaact
     1321 gggggagaac gacaaaaacc agatgctgta cagcatgaag gtgtgcccac cgctgtggcg
     1381 cacagggctg cgccagaact tccgtatctt ccagaatgag gacatcgaaa gcatcctcgg
     1441 cacgatattg caggaaaacg gggtgaccga gtggagcccg ctgttcagcg agccacatcc
     1501 ttcccgtgag ttttgtgtcc agtacggtga gactgattac gatttcctgt gccggatggc
     1561 ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa aaaagtaccg accagagcct
     1621 ggtcctgtgc gataccgtgc gttatctgcc ggagtccttt gagatcccct ggaatccgaa
     1681 cacccgtacc gaggtgagca ccctctgcat cagccagttt cgctacagcg cacaaatccg
     1741 cccttcttcc gtggtgacca aagactacac ctttaaacga cccggctggg cagggcgttt
     1801 tgatcaggaa ggccagtacc aggattacca gcgcacacag tatgaagtgt atgactaccc
     1861 cggacgtttc aagggtgccc acgggcagaa ctttgcccgc tggcagatgg atggctggcg
     1921 caacaacgca gaagtggcgc gcggaacaag ccgttcgccg gagatatggc cgggacggcg
     1981 aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg tggtggcaag
     2041 tgaactgcac ggcgaacagc cgcaggcggt accgggacgc aggggttcag gcaccacgct
     2101 ggataaccac tttgcggtaa tcccggcaga ccggacatgg cggccacagc cgttgctgaa
     2161 accgctggtg gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt
     2221 ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa
     2281 ccaggaaagt tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gctttggtaa
     2341 cctggcgata ccgcgtgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga
     2401 ccagccgatc attatggggc gtacctacca ccaggaaaac cgcacccccg gcagcctgcc
     2461 ggggacgaag acgcagatga cgatccgctc gaaaacctat aagggcagcg ggtttaatga
     2521 actgaagttt gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat
     2581 gaacaccgag gtgctgaata accgcaccac tgatgtgata aacaaccatg ccgaaaaaat
     2641 aggtaacaac caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca
     2701 gatacagacg gttggtgtca accaggtgga aacggtgggc agtaaccaga ttatcaaagt
     2761 gggatcaaac caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggctta
     2821 ccagacgacg gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt
     2881 agggctgcat aaatcactga tggtgggaat gggctacagc gtcaatgtgg ggaataacgt
     2941 caccttctcg gtgggcaaga caatgaagga aaacaccgga caaacagcag tttattctgc
     3001 cggtgagcat cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag
     3061 catatttctc aatggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc
     3121 gccagtgatt aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa
     3181 agatttaccc cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa
     3241 acggaactgg tgcagtgacc agataaacca tattaaaagc agaaggaatt gattcatggg
     3301 cggaaaaccg gcggcgcggc agggtgatat gacccgcaag ggactggata ttgtgcaggg
     3361 ttcagcgggg gtgctgatag gtgcgccgac gggagtggcc tgttcggtat gtccgggagg
     3421 gattacctat gctaacccgg tgaacccgct gctgggtgcg aaggtgctgc cgggcgagac
     3481 ggaccttgcg ctgcccggcc cgctgccgtt tattctttcc cgcgcctaca gcagctaccg
     3541 gaccagaaca cccgcgccgg tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat
     3601 ccgcttacag atacgcgatg aaggcctgat actcaacgac aacggcggca ggagcattca
     3661 ctttgagccg ctgtttcccg gcgagataag ctacagccgc agcgagtcac tctggctggc
     3721 gcggggcggg gtggcggagc agcacagttc gcagccgcta agcgcactct ggcaggtgct
     3781 gccggaagat gttcgcctga gtccgcatgt gtacctggcg acaaacagcc ttcaggggcc
     3841 gtggtggata ctcagctggc cggagcgggt gccgggggcg gacgaggtgc tgccgccgga
     3901 gccgcccgca taccgggtgc tgacgggcgt ggtggacggc ttcgggcgga cgctgacttt
     3961 ccaccgggcg gcggagggtg acgtggcggg cgcggtgacg ggggtgacgg acggcgcggg
     4021 ccgctgtttt cacctggtgc tgaccacaca ggcgcagcgg gcggaagcct tccgtaaaca
     4081 gcgcgccacg tctttatctt cccctgccgg tccccgttct gcttcctctt cttctgcttt
     4141 ccccgacacg ctgcccgccg gtacagaata cggtgccgat aacggtatcc ggctggaggc
     4201 ggtgtggctg acacacgacc cggcataccc ggatgaacag ccgactgcgc cgctggcgcg
     4261 ctacacgtac acggccagcg gagaactgcg ggcggtgtat gaccgcagcg ggacgcaggt
     4321 tcgcgggttt acttatgatg cggagcacgc cgggcggatg gtggcacacc attatgcggg
     4381 gaggccggag agccgctacc ggtatgatga taccggtcgg gtgacggagc aggtcaaccc
     4441 ggaggggctg gactaccgct ttgagtacgg gcaggaccgt gtgaccatca cggacagcct
     4501 gaaccggcgg gaggtgctgt acacggaggg cgagggcggc ctgaagcggg tggtgaagaa
     4561 ggaacatgcg gacgggagca ttacccgcag cgagtatgat gaggcgggga ggctgaaggc
     4621 acagacggat gcggcggggc ggcggacgga gtacagcctg catatggcgt cgggtgcggt
     4681 gacggcggtg acggggccgg acggcaggac ggtacggtat ggctataaca gccagcggca
     4741 ggtgacgtcg gtgacgtacc cggacgggct tcgcagcagc cgggagtatg atgagaaggg
     4801 aaggctggcg gcggagacct cacgcagcgg tgagacgacg agttacagct atgatgaccc
     4861 ggcgagtgag ctgccgacag ggatacagga cgcgacgggc agtacaaaac agatggcatg
     4921 gagccgttac ggtcaactgc tggcctttac ggactgctcg gggtacacga cgcggtatga
     4981 gtacgaccgg tacggtcaac aaatcgccgt tcaccgggag gaaggcatca gcacttacag
     5041 cagttataac ccgcgtgggc aactggtcag tcagaaggat gcgcaggggc gtgaaacccg
     5101 ttatgagtac agcgccgcag gagacctgac agcaacggta tcaccggacg gtaaacgcag
     5161 caccatcgca tatgataaac gcgggcgtcc ggtgagtgtg acggaaggcg gtctgacgcg
     5221 cagcatgggg tatgacgctg ccgggcgcat caccgtgctg accaacgaga acggcagcca
     5281 gtccacgttc cggtatgacc cggttgacag gctgactgaa cagcgcggtt ttgacggccg
     5341 gacgcaacgt taccactatg acctgaccag aaaactcaca cagagtgaag acgaggggct
     5401 tatcactctc tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga
     5461 cccggcagag cagtggcagt atgatgagca cgggtggtta accaccctca gccataccag
     5521 tgaaggccac cgcgtgtcgg tccactacgg gtacgacgat aagggccgcc tgacgggcga
     5581 acggcagacg gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca
     5641 cgcctacaac gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga
     5701 gtggctgacc tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt
     5761 cgagtatacg cgggacaggc tgcaccgtga gacggtgcgc agcttcggca gcagggcagg
     5821 cagtaatgcc gcatatgaac tgaccagcac atacaccccc gcaggccagt tacagagcca
     5881 gcacc
//
LOCUS       DAZUFB010000190         5769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZUFB010000190 DAZUFB010000000
VERSION     DAZUFB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.190"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..302)
                     /gene="escG"
                     /locus_tag="ACHY3M_004814"
     CDS             complement(24..302)
                     /gene="escG"
                     /locus_tag="ACHY3M_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFS4319571.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALENCMDDETSQLLYSLVHENGSGWVRGF"
     gene            complement(308..529)
                     /gene="escF"
                     /locus_tag="ACHY3M_004815"
     CDS             complement(308..529)
                     /gene="escF"
                     /locus_tag="ACHY3M_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFS4319572.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            complement(564..971)
                     /gene="cesD2"
                     /locus_tag="ACHY3M_004816"
     CDS             complement(564..971)
                     /gene="cesD2"
                     /locus_tag="ACHY3M_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFS4319573.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFIMNVNS"
     gene            complement(978..1940)
                     /gene="espB"
                     /locus_tag="ACHY3M_004817"
     CDS             complement(978..1940)
                     /gene="espB"
                     /locus_tag="ACHY3M_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFS4319574.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTGSASSTTTSTSSISSSLLTDGRV
                     DISRLLLEIQKLLREMVTTLQDYLQKQLAQSYDIQLAVFESQNKAISEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDAVQQAASTSAKSISTASEASTKALAKASESIA
                     DAADDAASVQQTIAAAAKAASRTSGVTDDVAASAQKASQVAEEAAGAAQELAEKAGLL
                     SRFTAAAGRISGSTSFIVVTSLAEGTKTLPTTISESVKSNHDISEQRAKSVENLQASN
                     LENYKQDVRRTQDDISSRLRDMTTTARDLTDLINRLGQSARLAG"
     gene            complement(1961..3103)
                     /gene="sctE"
                     /locus_tag="ACHY3M_004818"
     CDS             complement(1961..3103)
                     /gene="sctE"
                     /locus_tag="ACHY3M_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFS4319575.1"
                     /translation="MLNVNSDIQSVMSGVSAATTTSGINQPGGSSALDLQLVKSAAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGDVMSRTSGTEPQSSTQSTTSG
                     YPYLSQVNKVDPQTMMMMATLLSLDASAQKVASMKNSNEIYKDGQNQALENKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAVAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLMSKVGDVAGKVGSNIVKAVTT
                     LADTLVENVASKISAVANGLTTSSRSIGTTVLNNDAANHNLLSQISSFVVENLTRQSE
                     SLSQSAKAELDKAALEVQNQANYIQSASKLISDSARVNSRIVSGRV"
     gene            complement(3116..3694)
                     /gene="espA"
                     /locus_tag="ACHY3M_004819"
     CDS             complement(3116..3694)
                     /gene="espA"
                     /locus_tag="ACHY3M_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFS4319576.1"
                     /translation="MDTLNTASVASTNASTSTSLTYDLGSMSKDQVVELFNKLGVFQA
                     ALLMFSYMYQAQSDLSIAKFADMNEASKASTTAQKMANLVDAKIAEVQSSSDKNAKAQ
                     LPKEVLDYINDPRNDVTISGITDLNAALGAGDLQTVKAAISAQANNLTTTVNNSQLEI
                     QQMSNTLNLLTSARSDMQSLQFRTISSISLGK"
     gene            complement(3754..4809)
                     /gene="sepL"
                     /locus_tag="ACHY3M_004820"
     CDS             complement(3754..4809)
                     /gene="sepL"
                     /locus_tag="ACHY3M_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFS4319577.1"
                     /translation="MANGIEFNQNTASIFNSNSLDFELESQQLTQKSSSNSNSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLELLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEVKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRIIVFLSLDNYTDIIAISIKKDKDVI
                     LNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDNEQRD
                     NTLLMIGKVIDYKEDVM"
     gene            4952..>5769
                     /gene="sctD"
                     /locus_tag="ACHY3M_004821"
     CDS             4952..>5769
                     /gene="sctD"
                     /locus_tag="ACHY3M_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFS4319578.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPIEFWIDGQPTPYEADQPLPIGKVIDIAGCGFIIGDIDHALP
                     LSDMPERFSARNQRKKRLILAGVIGAAIALSGAIGSYVLLSPKAELPTFTRADVYQQL
                     KENKLYAITLVWHGKNIALYGRCESTTDLTPFFNYLKENNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHN"
BASE COUNT         1687 a         1299 c         1053 g         1730 t
ORIGIN      
        1 aaattatttt aaatcaatat aaattaaaat cctcgtaccc agccactacc attctcatgg
       61 accaaactgt acaataactg actcgtctca tcatccatac agttctccaa agcctttacc
      121 gcatcatttt ttctatttaa tccgaataag ataataaatt ctaaatgtgc gatatctttt
      181 ttatcaggta taatttgcgg aaatacattc aggatagctt ccgcatattt gggaagctta
      241 tgatttgccg cggctacagc tgcccacacc agtatcttat tggcagaaac atcattaacc
      301 ataaaaatta aaagctacgg ttggaaatgg ttgagaccag atcttttatc gttttcagca
      361 ttccactttc tacgttaaca taagcagaat attgctgaac cgcaaactgc aactctaaca
      421 ttttttcagg gtcattaacc aaatcggtgc tattaagtaa ctctggcaca gaatcgctca
      481 gtgttttacc tacctcaccc atttgttgag taatttcaga taaattcatt aaatctcctt
      541 aatagttaat aaaataaaaa tccctaacta tttacattca ttatgaacca aattccgtct
      601 tctgtttctt gctgcacggc accctttctg atgaaataat taaacaactc ttcgcgcttt
      661 tgacgttctg aatctgaacc aatcttagat ttcatcgcac tgattttctt aatacccgaa
      721 aaatggtgat acaactcttc gagcaaacga gtcagtgata aaacagggcc gttaagccct
      781 gtttggttac gtgcttcaaa agagcacaaa atcagttctt gttcatccag tctgtacgct
      841 aatgagaaaa aaggtgtctc aatccgccat ccgataaaaa aggcactgcc acaaagaaac
      901 tccttctgta ttgtgtaccc tttttgttca agatagtgat taagcacttc atcattaaac
      961 gtatcgacca tgatcaatta cccagctaag cgagccgatt gccccaggcg attaataagg
     1021 tcggtaaggt cacgggctgt cgttgtcata tcacgcagac ggctagagat atcatcctgc
     1081 gttctgcgaa catcctgttt gtagttctct aaattcgacg cctggagatt ttctacagat
     1141 ttagcacgct gctcactgat atcatgattg gacttgacag attcagatat cgttgttggc
     1201 aatgttttcg taccttcagc aaggctggta acaacaataa atgacgtaga accggaaatc
     1261 cttccagcag cagcagtaaa gcgacttaat aatcctgcct tctctgctaa ttcttgagca
     1321 gcaccagcag cctcttcagc tacttgagaa gctttctgag cagaagcagc cacatcatca
     1381 gtgacaccag atgtacgact ggccgctttt gcagctgccg cgatagtttg ctgcactgag
     1441 gctgcatcat ctgctgcatc tgcaatactt tcggaagcct tcgccagtgc tttagttgaa
     1501 gcttcagagg ctgtactgat agacttcgca gaagtagaag cggcttgctg aacggcatca
     1561 ctcgctcctt tcgttgcgct attaatggca gcaaaagagc ctaaaatccc caggacagat
     1621 gagatagcac caccaatcag agcagctgtc gcaccggctt ttttctcact aatagcttta
     1681 ttttggctct caaaaacggc tagttggatg tcatagcttt gcgccaattg cttctgaaga
     1741 taatcttgca atgtggtgac catctcacgc aacagttttt gaatctccag cagaagccta
     1801 gaaatatcca ctctgccatc agtaagtaaa gatgaactga ttgacgaggt tgatgtcgtt
     1861 gttgaactgg cgctgccagt cgtgctgctc aaaacagaat taactgcgat tgccgcattg
     1921 ttattatcga tagtattcat tataaacgcc tctttaacaa ttaaactcga ccgctgacaa
     1981 tacggctatt cactcgcgca gaatcagaaa ttagcttaga agcactctgt atataattcg
     2041 cctggttttg cacctcgagt gcggctttat ccagctctgc cttcgcactc tgacttaagc
     2101 tttcactttg tcgagttaaa ttttccacaa caaaagaaga aatctgtgat aacagattat
     2161 gattcgctgc gtcattattc agcacagttg taccaataga gcgcgaagaa gtggttaaac
     2221 catttgccac agccgaaatt ttcgatgcaa cgttttcgac aagagtgtcg gccaacgtag
     2281 tgaccgcttt tacgatgttt gaacccactt tgccagccac atcgccaact ttagacataa
     2341 gtgaagacac cccgccaatg ccggctgtca gaatacctgc ggcaagagat agaccgccaa
     2401 aagcttgcgc tgctgttttt aacgcctgcg gagccttatc gcccataaca tcaaccgcag
     2461 tttgcagcgc cattgctgtt gcactaacag caacaacagc ccagagagct gggttaaaaa
     2521 tagctgcaac agcagttacc gcaaccccta accaaccaaa gacctgacca acaattttac
     2581 ttttttgtgc tttctcttcg gctttctgtt gttcttcaag ctgtttttta aattccagag
     2641 ttttattttc cagcgcttga ttttgtccat ccttgtagat ctcgttggag ttcttcatac
     2701 tcgcaacttt ctgcgctgag gcatccaggg ataacagagt ggccatcatc atcattgtct
     2761 gagggtcaac cttattcacc tgagaaagat aaggatagcc agaggttgta ctttgtgtac
     2821 tactctgtgg ttctgtccca ctagtgcggc tcataacatc accactaatg ccgccaaaaa
     2881 gtttgctcaa aacatcttca gcaacagagg gcgtcactaa tgagtggcct gccggcggcg
     2941 ttggtaaagc ggtactttct gtccaacttg ctgatggggc agcggattta accagctgta
     3001 aatccagcgc ggaagaccct ccaggttgat taatacctga agtagtcgta gcggcactga
     3061 ccccagacat cacagactgg atatcgctat ttacattaag catagttatc tccggttatt
     3121 taccaaggga tattgatgat atagttctga attgcagtga ctgcatatca gaacgtgcac
     3181 tcgttaatag gtttaacgta tttgacattt gctgtatttc aagctggcta ttattcaccg
     3241 tcgttgtcaa attattcgct tgagctgaaa tagctgcttt caccgtttgc aaatcaccag
     3301 cgcctaatgc agcatttaga tcggtaatac cacttattgt aacgtcattg cgaggatcat
     3361 ttatatagtc aagcacttct ttaggaagtt gagccttcgc attcttgtcg gagctactct
     3421 gaacctcagc aattttagca tccacaagat tcgccatttt ttgggcagtg gttgacgcct
     3481 tagatgcctc attcatatca gcaaactttg caatcgacag atcgctttgt gcctgataca
     3541 tataggaaaa catgagaagc gcagcctgaa aaacaccgag tttattgaat agttcaacaa
     3601 cctgatcttt cgacatgctc cctaaatcat aggtcagcga tgtcgaagta ctcgcattcg
     3661 tactagcaac tgatgctgta tttaatgtat ccatgtatat acctcttaat aatctttttt
     3721 agcatgacaa aaactattaa aaaaacaatt aaatcacata acatcctcct tataatcaat
     3781 tactttgcca atcattaata atgtattatc cctctgctcg ttatcaataa aacaagcatc
     3841 gggtaatgat gcaaaaaagt ctttcaataa atggaaataa taaacatgtt tatcttcgac
     3901 tatcgatacc cgagaagggc tttccagtag ccagtcttct gttagccata catgttcaat
     3961 aatagataac acttcattta aaatgacatc tttatctttc ttaatggaga ttgcaataat
     4021 atctgtatag ttatcaagcg ataaaaaaac gattatccgg cataaatcgc taataattga
     4081 agatgttttt accttgtctt caaaagaaga catataagaa agatcgaacg atagcgccct
     4141 aataataacc tttaagtgct tctcacgctc tggaagatcc ttgacctcca taaaccagtc
     4201 aagtaaagag gctccgtttt cagagtcctc gtccttagcc ttttcataaa gcttcttgat
     4261 cttctggata ccttcctgat ttaactcacc cacccctaac caggaaagca gagccaactc
     4321 ccaatcatct tcgcctaata attctaataa taatttggca tatttctttt ttaattcaat
     4381 cttaacaaac tttgattgca atagagaaga tatgagcgcg acaatttccc cttttggcat
     4441 attaccaaac atagccaagt taggatcatg ctgttccagc agcccactgt tctttaatga
     4501 tagttctttt agtttatcgc tatcggtcag agtaagcagc tcttgcatat cattgagcag
     4561 cttctccgtt gtcgtacctt cctcttcttt cctggcgcta cctttgcgat accctaggct
     4621 tagcccttca atcgtttcag aaagcgacga actggtaatc atcgctaatt cattttgtaa
     4681 atttaataat ggagaattgc tattagaaga gcttttttgc gttaattgtt gagattccaa
     4741 ttcaaaatct aatgaattag aattgaaaat agatgcggtg ttttgattaa attcaatacc
     4801 attagccatt ggaaactcac gtaaacatta attatgaata ttccattaaa tagtactgta
     4861 caacagcttc ctttctactc tattcttagt cagtgtaagt tcaccatatt ttttctcatt
     4921 gttcaaccac caggacgaat aaaatttaaa aatgttatcc tcatataaaa taaaacttct
     4981 caatggtgca atgcgtaatc gggaattaca acttccgatg ggaaacctaa cgataggcac
     5041 agaagataac gatattgttt actttcccct tgaacaaggc ctcaatcagt ttttacttga
     5101 tatacgtgag gaaggtgttt tccttttatc tccaattgaa ttttggattg atggacagcc
     5161 cactccctat gaagcagatc aaccattacc tatcggaaag gtcatcgata tagcggggtg
     5221 tggcttcatc attggtgata ttgaccacgc cctgccattg tctgatatgc cagaaaggtt
     5281 ctctgccagg aatcaacgca aaaagcgtct tatcctggca ggcgtgattg gtgccgctat
     5341 tgcgttgtct ggagccatcg gcagttacgt tttgctttcc ccaaaagctg aactcccaac
     5401 gtttaccaga gctgatgtat atcagcagct aaaagaaaac aaactttatg caatcaccct
     5461 ggtatggcat gggaaaaata tcgcgcttta tgggcgttgc gaatcaacca cggatttaac
     5521 tccatttttc aattacctga aagaaaataa tattttttat tacaataata tcatttgcaa
     5581 taaccaaatc atatccgcaa ttaatgatat tttaacggaa tacggttata aagatatcat
     5641 cattacaaaa ggtaataagc ctggattctt tctgttatcg ggatacattc ccccttcacc
     5701 caaatggagc gaagttgaaa atctgttact gaatacgccg ggagttgctg gatgggaaat
     5761 acacaacaa
//
LOCUS       DAZUFB010000191         5720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZUFB010000191 DAZUFB010000000
VERSION     DAZUFB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.191"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            233..1165
                     /gene="rfaD"
                     /locus_tag="ACHY3M_004822"
     CDS             233..1165
                     /gene="rfaD"
                     /locus_tag="ACHY3M_004822"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFS4319579.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1175..2221
                     /gene="rfaF"
                     /locus_tag="ACHY3M_004823"
     CDS             1175..2221
                     /gene="rfaF"
                     /locus_tag="ACHY3M_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFS4319580.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2225..3217
                     /gene="rfaC"
                     /locus_tag="ACHY3M_004824"
     CDS             2225..3217
                     /gene="rfaC"
                     /locus_tag="ACHY3M_004824"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFS4319581.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3214..4422
                     /gene="rfaL"
                     /locus_tag="ACHY3M_004825"
     CDS             3214..4422
                     /gene="rfaL"
                     /locus_tag="ACHY3M_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFS4319582.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4458..5600)
                     /locus_tag="ACHY3M_004826"
     CDS             complement(4458..5600)
                     /locus_tag="ACHY3M_004826"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFS4319583.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5609..>5720)
                     /locus_tag="ACHY3M_004827"
     CDS             complement(5609..>5720)
                     /locus_tag="ACHY3M_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFS4319584.1"
                     /translation="KAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1579 a         1253 c         1276 g         1612 t
ORIGIN      
        1 ccattcaaat gcttccgtta tttatcatac atcggaatat tgatactaaa gcactattca
       61 catgcaaaac caacatccgc catgaaggac tagctaaaac ccaaactagt ttgttgcaat
      121 tagcatcctt gcacctctat gtaaagggct gaagggattc ggatgtgatg gtatgattac
      181 agacattcgt gtctgagatt gtctctgact ccataattcg aaggttacag ttatgatcat
      241 cgttaccggc ggcgcgggct ttatcggcag caacatcgtt aaagccctga atgataaagg
      301 catcaccgat attctggtgg tggacaacct gaaagacggc accaagtttg tgaacctggt
      361 ggatctgaat atcgcagact atatggataa ggaagacttc ctgatccaga ttatggctgg
      421 cgaagagttc ggcgatgtcg aagcgatttt ccacgaaggc gcgtgctctt ccaccaccga
      481 gtgggacggc aagtatatga tggataacaa ctatcaatac tccaaggagc tgctgcacta
      541 ctgcctggag cgtgaaatcc cgttcctgta cgcttcttcc gcagccacct acggcggacg
      601 cacctccgac tttattgaat cccgcgagta cgaaaaaccg ttgaacgtct acggttactc
      661 aaaattcctg tttgatgaat atgttcgcca aatcctgccg gaagcgaact cgcagattgt
      721 tggcttccgc tatttcaacg tttatggacc gcgtgaaggc cataaaggca gcatggcgag
      781 cgtcgctttc catctcaaca cccagcttaa taacggtgaa tcaccgaagc tgttcgaagg
      841 tagcgagaac tttaaacgcg acttcgtcta tgttggcgat gtggcagatg taaacctgtg
      901 gttcctggaa aatggcgttt ccggcatctt caacctcggc accggtcgtg cggaatcctt
      961 ccaggctgta gctgatgcta cgctggctta tcacaagaaa ggccagatcg aatacattcc
     1021 gttcccggat aagctgaaag gccgctacca ggcgttcact caggcagatc tgacaaatct
     1081 gcgcgcggcg ggttacgaca aaccgttcaa aaccgttgct gaaggtgtaa cggaatacat
     1141 ggcctggctg aatcgcgacg cataagagct ctgcatgaaa atactggtga tcggcccgtc
     1201 ttgggttggc gacatgatga tgtcgcaaag tctctatcgc acgctccagg cgcgctatcc
     1261 ccaggcgata atcgatgtga tggcaccggc atggtgccgt ccattattat cgcggatgcc
     1321 ggaagttaac gaagctatcc ctatgcctct cggtcacgga gcgctggaaa tcggcgaacg
     1381 ccgcaaactg ggtcatagcc tgcgtgaaaa gcgctacgac cgcgcctacg tcttacctaa
     1441 ctccttcaaa tccgcattag tgcctttctt cgcgggtatt cctcatcgca ccggctggcg
     1501 cggcgagatg cgctacggtt tactcaacga tgtacgcgtg ctcgataaag aagcctggcc
     1561 gctaatggtg gaacgctata tagcgctggc ctatgacaaa ggcattatgc gcacagcaca
     1621 agatctgccg cagccattgt tatggccgca gttgcaggtg agcgaaggtg aaaaatcata
     1681 tacctgtaat caattttcgc tttcatcaga acgtccgatg attggttttt gcccgggtgc
     1741 ggagtttggt ccggcaaaac gctggccaca ctaccactat gcggagctgg caaagcagct
     1801 gattgatgaa ggttatcagg tggttctgtt tggctcggcg aaagatcatg aagcaggcaa
     1861 tgagattctt gccgctttaa ataccgagca gcaggcatgg tgtcggaacc tggcggggga
     1921 aacacagctt gatcaagcgg ttatcctgat tgcagcctgt aaagccattg tcactaacga
     1981 ttctggcctg atgcacgttg cggcggcgct caatcgtccg ctggttgccc tgtatggtcc
     2041 aagtagcccg gacttcacac cgccgctatc ccataaagcg cgcgtgatcc gtctgattac
     2101 cggctatcac aaagtgcgta aaggtgacgc tgcggagggt tatcaccaga gcttgatcga
     2161 cattactccc cagcgcgtac tggaagaact caacgcgcta ttgttacaag aggaagcctg
     2221 acggatgcgg gttttgatcg ttaaaacatc gtcgatgggc gatgttctcc atacgttgcc
     2281 cgcactcact gatgcccagc aggcaatccc agggattaag tttgactggg tggtggaaga
     2341 agggttcgca cagattcctt cctggcacgc tgccgttgag cgagttattc ctgtggcaat
     2401 acgtcgctgg cgtaaagcct ggttctcggc ccccataaaa gctgaacgca aagcgtttcg
     2461 tgaagcgcta caagcagaga actatgacgc agttatcgac gctcaggggc tggtaaaaag
     2521 cgcggcactg gtgacacgtc tggcgcatgg cgtaaagcat ggattggact ggcaaaccgc
     2581 tcgcgaacct ttagccagcc tgttttacaa ttgtaagcat catattgcaa aacagcagca
     2641 cgccgtagaa cgcacccgcg aactgtttgc caaaagtttg ggctatagca aaccgcaaac
     2701 ccagggcgat tatgctatcg cacagcattt tctgacgaac ctgcctacag atgctggcga
     2761 atatgccgta tttcttcatg cgacgacccg tgatgataaa cactggccgg aagaacactg
     2821 gcgagaattg attggtttac tggctgattc aggaatacgg attaaacttc cgtggggcgc
     2881 gccgcatgag gaagaacggg cgaaacgact ggcggaagga tttgcttatg ttgaagtatt
     2941 gccgaagatg agtctggaag gcgttgcccg cgtgctggcc ggggctaaat ttgtagtgtc
     3001 ggtggatacg gggttaagcc atttaacggc ggcactggat agacccaata tcacggttta
     3061 tggaccaacc gatccgggat taattggtgg gtatgggaag aatcaagtag agtgtcgttc
     3121 tacaagtatg tctcttgcag atttgccagc tcaaaccgtt tttcaaaatt taaatcttga
     3181 aataataacc aataagttga catcggagat aagatgacct caacattatt tttctctctc
     3241 gagaaaaaaa actggatagc gtactggaac agagctctcg tattcttatt cattaccacc
     3301 tattttttgg gtgggattac aaggtataaa catcttattg ttattcttat gacaataacg
     3361 acaatcgtct atctctgcaa acggccaaaa cactatctct cactatttaa aacatttctt
     3421 tttggtagtg ttgccatatt aactattgct gcattgctgt cacttcttca atcccctgat
     3481 gcaggtgcta gcatgaagga aattttcaaa gctattattg agaatacttt actatgcaca
     3541 atagcaatac cgatcatatt gagagacgag aaaagagaag atgtcgaaaa aatcgttttc
     3601 ttctcattta ttagtgcgtt gggcttacgc tgtttttctg aattgattac ctattataag
     3661 gactatcaac aagggataat gccattcgca gattatagac accgtagcat ttctgactcg
     3721 atggtctttt tattccctgc attgttaaat ctctggctta tcaaatcagc aaaataccgc
     3781 atttcttttg tggttctaag cgttattttt atttttctga tattaggaac tttatccaga
     3841 ggggcctggc tttccgtgtt agtcattgga ttaatatgga ttctgatgtt taaacaatgg
     3901 aagttactat tagtaggagt aatggttgcc atcattgcat tgtcggttat tttcacacat
     3961 aaggagatga ccgcaaagct aacgtataaa cttcaacaaa ctaatagttc ttatcgctat
     4021 gcaaatggta ctcaaggcag cgcactcgat ctaatattag aaaaccctgt tattggttat
     4081 ggttacggta acgttgcata taaagatgtc tataataaac gtgtcattga ttatccagaa
     4141 tggaccttta gacaatcaat agggccacat aattttgcgc tattcatctg gtttggcact
     4201 ggtttattag ggctggtaag tcttatgatg ctatactgtg caatattgaa agagtgtata
     4261 aaaaatggcg tcaagaataa atatcgctca ccatataatg catattatat aatcttacta
     4321 tcttttatag gttattttgt tatccgtgga aacgtagaac aaattgaacc aaatttatta
     4381 ggcgtttacg ccggcttatt attagcgatg aaaaacaagt aaaaaataaa aaaggctgca
     4441 taatgcggcc tttttattta ttcaaaccaa ttatgaataa cctcttcgaa tctttgagtt
     4501 acgccatccc aactgtagtg ttcaaacaca aagtcctgac cttgctttgc aactgctgtt
     4561 aattcaggat tcgctaacgt ttttaaaata tcgctgctta tggagtctgc agtcataggt
     4621 tcttttaaat ggaatcccgt agtattttct ttgacaaatt ccgtcatccc cccacgcgta
     4681 ctcactaaaa caggcttacc agcacccata gcttcaattg ccaccatgca aaatggttcc
     4741 tggaactggg aaggaatgac gactaaatct gccagtgggt aataacaatg catcttttca
     4801 ggcggtatgc ttcctaacat aatacaacga tccttcaaac gttttgcaat ttccctgaca
     4861 ttgcgttgat atgcaccttt gtcactttta ctcatttctg tataatcacc aatcacaact
     4921 aattttaaat ttttgtgagc agcggccaat ttttcgaatg attgcatcaa cagcaatatg
     4981 cctttgtcag ggacgatacg cccagcataa aaaataattt tctcttcagg ggagatatta
     5041 agttcgcttc tagtgatcgg ctgaaaatta gattgatagg tttctaaatc aattccattt
     5101 ggtacaatct caatatctgc atttgctaaa taagattggt aatacttttt caagtataaa
     5161 ctaggcacta tcattttaac attttgctca agcaattttg gttcaaatgc attatgcata
     5221 tgtataacca ctcttgcctg aggagctcgc ttacgaattt gagtgtatag cttcatgcta
     5281 ttatgaacta caataacact ttctttcgtt ataggaaaat catgtgcaat attcaatatt
     5341 ctctgtgaat atggaagagg atcaagtctt gtccattttt gaaataatcg cttatatatg
     5401 cgactgaatc caaccctatg aatactacaa ttatcattaa caaaggtgta atttgagtat
     5461 cctggatttt tgatacaaac aatacgattt ggaaaattgg tacgctgagc aacctgatac
     5521 atccacgttt caacagcagc cgctccgcga ggaggaattg aaaaaatagg agtaaccgta
     5581 aatattattt tatcaaccat ctcgtatatt aacctttcat aacattatat ttaattgctg
     5641 taaaaacacc atcgagaaat tttttctggt aaagcaagtg tttatatttt tctttgtact
     5701 catgtttttt aacagctttt
//
LOCUS       DAZUFB010000192         5681 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZUFB010000192 DAZUFB010000000
VERSION     DAZUFB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.192"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..227
                     /locus_tag="ACHY3M_004828"
     CDS             <1..227
                     /locus_tag="ACHY3M_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4319585.1"
                     /translation="NSPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGD
                     FQYAQCFGAPIVCEATHWMPLPEPPKEVRQ"
     gene            complement(433..732)
                     /locus_tag="ACHY3M_004829"
     CDS             complement(433..732)
                     /locus_tag="ACHY3M_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4319586.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGIYHGGQSYETDFL"
     gene            complement(738..995)
                     /locus_tag="ACHY3M_004830"
     CDS             complement(738..995)
                     /locus_tag="ACHY3M_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS4319587.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            1131..1409
                     /locus_tag="ACHY3M_004831"
     CDS             1131..1409
                     /locus_tag="ACHY3M_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319588.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFYRRHWIISVRLPYSECPPRPSRTFQQRIWR"
     gene            1411..2460
                     /locus_tag="ACHY3M_004832"
     CDS             1411..2460
                     /locus_tag="ACHY3M_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319589.1"
                     /translation="MRVLLRPVLVPELGMVVLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            2473..2847
                     /locus_tag="ACHY3M_004833"
     CDS             2473..2847
                     /locus_tag="ACHY3M_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319590.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2844..3665
                     /locus_tag="ACHY3M_004834"
     CDS             2844..3665
                     /locus_tag="ACHY3M_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS4319591.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3892..4089
                     /locus_tag="ACHY3M_004835"
     CDS             3892..4089
                     /locus_tag="ACHY3M_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4319592.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            4240..5298
                     /locus_tag="ACHY3M_004836"
     CDS             4240..5298
                     /locus_tag="ACHY3M_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS4319593.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWLLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            5344..5414
                     /locus_tag="ACHY3M_004837"
     tRNA            5344..5414
                     /locus_tag="ACHY3M_004837"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5372..5374,aa:Met,seq:cat)
     gene            complement(5518..5652)
                     /locus_tag="ACHY3M_004838"
                     /pseudo
     CDS             complement(5518..5652)
                     /locus_tag="ACHY3M_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1415 a         1312 c         1599 g         1355 t
ORIGIN      
        1 gcaattctcc ggtaactccg gatggttgga taagctgtag tgagcgaatg ccgccagttg
       61 gtgttgatgt acaactgttt gtgtccagca cgggtgaaca gtttacaggt tttaacctgg
      121 ataatacggg tgacttccag tatgcgcagt gtttcggtgc gcccatcgtg tgtgaagcca
      181 cacactggat gccgctacca gaaccgccga aggaggtgcg ccaatgagct ggcctgaagc
      241 cttctcaaat gttggtattg caatggcggt ggcgctggtt gtgtattcga tttgccgctg
      301 gggataattt gctgaaaaaa gatcccgaca cacacatgag tcggggatct ttgatttata
      361 tagcctacga atccgccagt aagagagggg gcggacggtt tattctaata ctggaattat
      421 gtggggaaaa gtttataaga aatcggtttc ataactttgt ccgccatgat aaatacctgc
      481 gataaagact ttattgccat caacggcaaa agcaataatc gttctgtggc ggaagtgagt
      541 tacccgcatt ccctgtcgaa tatcatcgcg tttattgccc cgatgcggga atgtggaaaa
      601 cccatcaaga taatcaagaa gtgcattggc ataattgtca gcaatgacgc tccctgcttt
      661 ctccgttata tatctgtgca ggttgattat ctgctgttcg gcctcaggag taatgatgac
      721 ttcatatgtc atgcagatta cttcccggct cgaatcgcgg cgcgaacctg tgaaatggag
      781 cgtccgttgt ttgggttttc gcggatagaa tcgagagatg gggcggctga atgcgttaac
      841 cacgcttcga ttgctttatc gcgctcattc agtgcgcgaa gcccttcacg aatgacctca
      901 ctttctgaag cataggcacc ggaagccaca cgggcgcgca ccatgtcagc catctcgtta
      961 gttaatgtaa tgctgaattg ttgggttgta cgcatggtaa acctcccgga gtaggataga
     1021 acactattcg atgatagcac actgcctgtt gacgacaaca gaaatcagat acaatattgc
     1081 cgcacgccag cctgaacaac tggcacctgc tgcgccagca gagacaaccg atggcgcacg
     1141 ataccagatt ttacaattcg gataactccg ccgcccctgc cagcaggcat gggcggcgtt
     1201 ctcatgcatt caaatctgac tggttccagc atgacccatg cactgaagaa caggccgaat
     1261 ggctgatcca gtgctaccgc agacgtggtt acgagtttca gaaagatctc agtttctatc
     1321 gtcgtcactg gataatctct gtcaggctcc cttattccga atgcccaccg cgtccgtccc
     1381 gcacattcca gcagcgcatc tggaggtaac gtgcgggtat tacttcgacc tgttctggtg
     1441 ccggaactcg ggatggtggt ccttaagccg ggccgtgaat caatgtcagc attccataac
     1501 ggcagaatat tggtggagcc ggaaccgaaa aacatgcgcg gtctgccgtc cggagtcgtt
     1561 cccgccgttc gccagccgct ggcagaggat aaaacattac tgccattttt cagcgatgag
     1621 cgggttattc gtgctgctgg tggtgctggt gcactgtctg actggttatt acgtcacgtg
     1681 aaatcctgcc agtggccaca cggcgattat catcacagcg aaacggtcat tcaccgttac
     1741 ggtaccggcg cgatggtgtt gtgctggcac tgcgacaacc agctgcgcga ccagacatca
     1801 gaatcactcg atcaacttgc tcagcagaat ctggttgcct ggatgattga tgtcatccgt
     1861 cacgcaataa gcggtacgca ggagagggag ttatcgctgg ccgaattatc ctggtgggcg
     1921 gcctgcaatc aggtggtgga tgcactacct gaggcagtag cgcgtcgttc gctgggatta
     1981 ccggcggaaa aaatccgctc cgtataccgt gagagtgaca tcgtaccggg agaacagaca
     2041 gccatcagca tactgaagca gcgcacaaaa aatattgcgc tgccacttca cgtccaccag
     2101 caacaaaatc caccacagaa aaaaacggtt gtcagtatcg ccgttgatcc ggagtctcct
     2161 gaatcgttca tgaggcggcc taaacgtcgc cgttgggtta atgagaaata cacgcgctgg
     2221 gtaaagacac agccgtgtgc gtgttgtggt aagccagctg acgatccgca tcacctgatt
     2281 ggtcatggtc aggggggagt gggaacaaag gcccacgata ttttcacgct accgttgtgc
     2341 cgggagcacc acaacgaact tcatgcagac ccgctgaagt ttgagaaaaa gtacggctct
     2401 caggttgagt taatttttcg ttttcttgat cacgcctttg cgactggcgt gctcgggtaa
     2461 aagaggtgac tgatgctcat agatttggtt ttaccttacc cgccgacggt gaacacttac
     2521 tggcgacgcc gtggcagcac atattttgta tcaaaagccg gggagcgtta tcgccgggca
     2581 gtggcgctta ttgttcgcca gcagcggctg aaattaagcc tgtccggacg gctggcaata
     2641 aaaattattg cagaaccacc ggataagcgc cgccgtgacc tggacaatat tctgaaagca
     2701 ccgctggatg cgctgacgca cgcggggttg ttaatggacg atgagcagtt tgatgaaatc
     2761 aatattgtac gtggaaagcc agtatctggt ggacgtctgg gggtgaagat ttaccccata
     2821 atgcttgaag ggcaggttaa aaaatgaaac tggaagattt accgaaatac tactccccaa
     2881 aatcccctgg cctgaccgat gcatcggcct caacgtcaaa agatgcgctg agtatcactg
     2941 atgtgatggc cgcgcagggc atgacacaga atcgggctga gatggggttt tctgcgttcc
     3001 ttgggaaaat gggcattagt atgaatgaca gagagcgggc aacagaattg ctgacagaat
     3061 atgcactcag tcggtgtgat cgcgtggcgg cgttaagaaa actcccggca gaaataaaac
     3121 cggcagtgat gcgtattatg gcttcgtatg cgtttgaaga ttatgcccgt agcgcggcga
     3181 gcaaaaaaca gtgcccctgc tgtcacggaa aaaaatttat tgaaagcgag gtttttacaa
     3241 acaagatcca gtatccggat ggtaagccgc cagtgtgggc aaagtgcaca aaaggcgtgt
     3301 atccgtctta ctgggaggaa tggaaaaaag tcagggaggt ggtaaaagtt gcctgtccgg
     3361 agtgtggagg gaagggagag gtatccactg cctgtaaaga ttgtcgtggg cgcggtgttg
     3421 ccattcatcg tgaagagtcg gaaaaacagg gtgtgccggt tttcagaaac tgtcagcgtt
     3481 gtggtgggcg tggctatgaa agattacctt caacggaggc atttaatgcc atatgtaatg
     3541 taaccgatgc catatctctt gatacatgga aaaaaacagt taaacgtttt tacgatacgc
     3601 tggtggtgca gtttgatatt gaagaagcat gggcagaaca acaactgaaa aaggtgacca
     3661 gatagctttg ttgatttttc ccgaatctgt ggtaaatttg ccctaacgat gggcgtttta
     3721 tgcctgacgt tagaagattt tttacacccc gccgcctggc gggtttttta tgactgaaat
     3781 cgcgtcagta cagtaaacgc gctggtggcg gtgaataccg gtctttcagc ttgctggctt
     3841 ttttgacaag agttattggt gtgtcacgtt aaccggaaaa gggaaaaaag catgctaaaa
     3901 cagcaggata tgacagaaac cgccagagca gtgtttaatg aattaagcgt caccgaaccg
     3961 gcgacagtcg gggagattgc gcagaatact tacctttcac gcgaacgctg ccagttaata
     4021 ctgacccagc ttgttatggc gggtctggca gactatcagt tcggttgtta cagacgcctt
     4081 cagtcctgaa ggctttttta tttgtggtaa atgggcggct ggtgggtgtt aggggcactc
     4141 accagccatc tgctcatgcg tctggatcac aagcaaacct caggcccact gctttgcgca
     4201 aaagcagaat gagcctatca gagacaggct taatgatcca tgtttaacac tgtaaaaata
     4261 tccagttgtg agttgatcaa cgctgactgc ctggaattta tccggtcgtt acccgaaaat
     4321 tctgttgacc tgatagtcac ggacccgccg tactttaaag tgaagcctga gggctgggat
     4381 aaccagtgga agggcgacga tgattacctg aagtggctgg accagtgtct ggcgcagttc
     4441 tggcgggtgc tgaaacctgc cggaagtctt tacctgttct gtggccatcg cctggcatct
     4501 gacattgaaa tcatgatgcg tgaacgcttc agtgtgctga accatattat ctgggcgaag
     4561 ccgtccggac gctggaacgg gtgcaacaag gaaagcctgc gggcgtattt ccccgccaca
     4621 gagcgcattc tgttcgcgga acattatcag gggccgtatc gtccgaaaga tgccgggtat
     4681 gaggcgaagg gcagggcact gaaacagcat gtgatggctc cgctgatttc ttactttcgt
     4741 gatgcgcgcg ctgtcctggg gataacggca aaacagattg cagatgccac aggaaagaaa
     4801 aacatggtgt cgcactggtt cagtgccggt cagtggctgc tgccgaacga aagcgattat
     4861 ctgaaattac aggcactgtt tgcccgggtg gcagaagaga agcatcagcg cggtgaactg
     4921 gaaaagcccc accaccagct gctggagacg tatacttcac tgaaccggca gtatgcggaa
     4981 ctgcagagtg aatataaaca tctgcggcgg tattttggcg tgacggcgca ggtgccgtac
     5041 acggatgtgt ggacgcataa accggtgcag tactatcccg ggaaacatcc gtgcgaaaaa
     5101 ccggcagaaa tgctgcagca gataatcagt gcgagcagtc gtccgggtga cctggttgca
     5161 gatttcttca tggggtcggg ttcgacagtc aaagccgcga tggcgctggg gcgtcgtgca
     5221 actggcgttg agctggagac tgaacgtttt gagcagacgg tcagggaagt acaggattta
     5281 gtcagtcaga acggatgata ttgcaggatt agttacgtac cgttattatc ctgcgcccgg
     5341 ccctttagct cagtggtgag agcgagcgac gcataatcgc caggtcgctg gttcgaatcc
     5401 agtacaacgc gccacactta ttttccctgg ctcgcttttg cgggcctttt tttaaatgtc
     5461 tcacaattca ggcggttgac tgttgtctgg tttgcgggga gtttgttaaa agaaactggc
     5521 atggtgaatc cccctgtgcg gaggggcaat cagcaaccag gtatatgggg taatcgcgga
     5581 ttcaggtgct ggtactgaat tcaccgggag gcacccggca ccatgcaaga aaaagaatgt
     5641 gcatgcaaac atgcccctct ccggaggggc ttttttatgg g
//
LOCUS       DAZUFB010000193         5569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZUFB010000193 DAZUFB010000000
VERSION     DAZUFB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.193"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(644..727)
                     /locus_tag="ACHY3M_004839"
     CDS             complement(644..727)
                     /locus_tag="ACHY3M_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4319594.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHD"
     gene            complement(1347..1538)
                     /locus_tag="ACHY3M_004840"
     CDS             complement(1347..1538)
                     /locus_tag="ACHY3M_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFS4319595.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            1606..1929
                     /locus_tag="ACHY3M_004841"
     CDS             1606..1929
                     /locus_tag="ACHY3M_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4319596.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            2138..2572
                     /locus_tag="ACHY3M_004842"
                     /pseudo
     CDS             2138..2572
                     /locus_tag="ACHY3M_004842"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2607..3167
                     /locus_tag="ACHY3M_004843"
     CDS             2607..3167
                     /locus_tag="ACHY3M_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFS4319597.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            3174..3407
                     /locus_tag="ACHY3M_004844"
     CDS             3174..3407
                     /locus_tag="ACHY3M_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFS4319598.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
     gene            complement(3887..4075)
                     /locus_tag="ACHY3M_004845"
     CDS             complement(3887..4075)
                     /locus_tag="ACHY3M_004845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319599.1"
                     /translation="MYTYEWAFFDKNGDKLPSAVTTEQRTYAAGDVKNYNGKNYKIVH
                     RIIDRVTKDSDLNIAVEV"
     gene            4509..5021
                     /locus_tag="ACHY3M_004846"
     CDS             4509..5021
                     /locus_tag="ACHY3M_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319600.1"
                     /translation="MSEKAKRSGRRTLATKRENLIAGLWGDELEELNIWSRHEHDGFT
                     TLPRTLTYINRILDFLGGSGSPLSQTYLALWCRVFDEGFVEIRDKEAFAYESGFSGQR
                     AVTTWSGRMKKLKELGFILTKPGTSGEFQYVIILNPFPVIKNIYENKEKDERYNALVG
                     RMQEVGAKWE"
     gene            5026..>5569
                     /locus_tag="ACHY3M_004847"
     CDS             5026..>5569
                     /locus_tag="ACHY3M_004847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319601.1"
                     /translation="MANYFPDLVISEVNYDFSHLDPFVMVVKSDMARKELKVHVRFTN
                     HCFTKGYDAFSHPAGEPIIPDHSGNKRTFCPIRYRLSLSLPAIISSLVNPQEKVFQTA
                     ARRNWAYSITIDDPAGPYHLFFEIRKASRAERHLQDLNVVVESAYHEEKGYGPPDLLG
                     RIGFVALCGRVYEGKPLATKR"
BASE COUNT         1435 a         1227 c         1534 g         1373 t
ORIGIN      
        1 aaaaaaaaag cacccaagag gtgccttttt tttatctttg aatcgttccg gacaccacta
       61 gcagttgctt cggtccgcct tgcgcgaaca caggatcgtt tcttatcctg ctgggtagcg
      121 ctcaatctca tccggtcagt cgctacgacg tgatcaatat aacaaaccct atatggggat
      181 tcagctcaaa ctacagagcg cagcattgga gcacttcgag cacctatcag tacagcaaac
      241 gcatttaagt gtttgatttt attatgcgct ttgttttatt ttttacatct caatgattga
      301 gtcggttgta taaaaatata tatattttat gtctgttatt ttgtttttct aaggagagag
      361 ccatgttgaa gaggcgctta ttctcttgag gagattacgg ttcctcttga gttcgtcttc
      421 tgctgcgcgt aatcttctgc tctgtaaacg aaaaaaccac cctggcaggt ggtttttcga
      481 aggttagtta atcctggcag attctctaac cgtggtaaca gtcttgtgcg agacatgtca
      541 ccaaatctgt ccttccagtg tagccgtagt aaggccacca cttcaagaac tctcgataca
      601 tctctcgcac atcctgttta ccaatggccg ttgccaatgg cgattagtcg tgtctttccg
      661 ggttggactc aagacgatag ttaccggaaa gggcgcagcg gtcggactga acggggggtt
      721 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagagtc aggcgtggaa
      781 tgagaaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
      841 agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct gtcgggtttc
      901 gccgccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
      961 aaaacggcaa cgccgcatcc ttgctccccg tccttcctga tttgagagcc atctctttat
     1021 cccgccattc gtgagccggt aacgcccgcc gcagtctcac gaccggagcg tagcgaccga
     1081 gtgagcgagg aggcgtcatt tcatcctcac ctgtatattg cacttacgcc ctgtagccac
     1141 atttttccct ttccacatga agcactttcc tgaatcccta cttcgattta ccatattaag
     1201 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcta
     1261 aaacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctca caggccagct
     1321 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcccc gaagcgtgcg
     1381 aggcagctgc ggtaaagctc gtcagcgtgg tcgtgaagcg cttcgcagat gtcagcctgg
     1441 tcgtcggcgt ccagttcatt cagtttttcc agcagggtca gcgtctggct tctgatgaag
     1501 cgggccatgt taagggcggt tttttcctgt ttagtcacag ttctgtttct ccatgtgtgg
     1561 ggagtctgtc ggaggttttt aatgataccg gtgaaacgcg aaaggatgct cacgatacgg
     1621 gtcacggatg aggaacatgc gcggctgctg gcacgttgtg agggaaaaca acttgcgtca
     1681 tggatgcgta aggtctgcct gggtgcaccg ccatcgaaaa cgtcaggtct tccgacactg
     1741 gcaccgccgc tgttgcgcca gttcgcttcg gtcggcaata acctgaatca gatagcccgg
     1801 aagataaaca gcggacactg gtcggggcat gaccgggttc atgtggtggc tgcactgatg
     1861 gccatcggac gtgaactgag tgagctgagg gatgaggtcc gtaaacaggg agaacgggat
     1921 gatagttaag tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct
     1981 gggccgggag cgtaaccggg aaggggcgag aattctgcgg ggtgatcccg aagaggtgcg
     2041 ggaactcatc gatgccacgc cgtttgcgaa aaaatacacc tcgggggttc tgtcttttgc
     2101 agagcagaca ctgccaccgg gagagcgtga aagggtgatg gagagctttg agcgggtact
     2161 gatgcccggt cttgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg
     2221 gcgtcttgag ctgaattttg tcatcccgaa tatggagctg gtgagcggaa aacggctcca
     2281 gccctattac gaccgggcgg accgtccgcg aatcaatgcc tggcagacac tggtgaatca
     2341 ccattacggg ctgcacgacc cgaacgcacc ggagaacagg cggacacttg tcaccccgaa
     2401 taatctgccg aaagcgaaac aggaggccgc agaagcgatt acgaggggac tggaggctct
     2461 ttaccatgct ggagcgctaa aaacgcgtca ggatgtcact gaggcgctta cagcggcagg
     2521 ttttgaggtg gtgaggacaa ccaggagcag tatcagtatc gccgacccgg agggcgggcg
     2581 taatctcagg ctgaaagggg cgctgtatga gcaatctttt acaaatgggg acagacttcg
     2641 agaagaaact gaaagagcgt gccgtctcta cagagaacat gctgaacagc gagttcagga
     2701 agctcgaagc gtctgtagac agggaactga acttaaacgg gcagaaaatc agagacgcta
     2761 tcagcgcaca cacagcagcg gtgaaagaac aactggagac gctgaacagt gccgtgaacg
     2821 cgcagttcag cgccacagag aacgaacttg cccggcggca ggaagagctg ctctggaagc
     2881 tggtcaaagg gcggatactg tacccgtcgc tgacggcgct ttgcgtcacg gtcgggattt
     2941 ttctggcgtc atgggggctg attcagtggc aggaaagccg tattgcggcg aacatcctgg
     3001 ctatcaggga ccaggaagag acgctggcga aactccggga aaaaacatgg ggtgtgacgt
     3061 atcacgaggg caggaacggg aaatttctgg tgttgccgtc cggggtgaag ggggaaaaca
     3121 actggacggt ggagaagaag aacgcagtaa gactggtcag ggagtaagtg agtatgacag
     3181 agctggaaat gcacttactg aacgccttag agcaattgca gcaggactat acgcagcggc
     3241 tgaaagactg ggagagcgcc ttcacggatt tgcagcatat gttcgagctg acacgacagg
     3301 agaacgggca actgagggaa cagtgcgacg gcttgagtca gcaggtgcag agattagccg
     3361 ggcaggtgga ccgcttgagt cggttattta tcacgaacag cagataaggg atgaacagga
     3421 aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga
     3481 agcacgggta tggcgtggcc cgtcaatggg gatgtgacgg tgagttgttc cgggaggttg
     3541 ccagatggta ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg tgtcgttttt
     3601 gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgcgatgt
     3661 ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc
     3721 aaaaccagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc
     3781 gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc
     3841 gctgaacgca tttcagcggt gcgggcaagg gatgttatgg taaatatcaa acttcaacag
     3901 caatgttgag gtcactatct ttagtcactc gatcaatgat cctgtgaaca attttatagt
     3961 ttttgccatt gtagtttttc acatcgcccg cggcatacgt tcgctgttca gttgttactg
     4021 ccgagggaag tttatcgccg ttcttatcaa agaaagccca ttcataagta tacatgtatc
     4081 atcctttaag ttgttgttat ttatattaat tataccacaa tcttttttcc cgtttcattt
     4141 atttcaatgc agattcatga gcagttcgct gctgggattt tcaatcaagc aagccagtaa
     4201 tttctaatca tctccctata gaaaatgagt tagctgcccc cttgcatccc cattcagcac
     4261 agcgagcaca agtcagcaca ttcagcacat tcagcacagc gagtacagta aaccaattta
     4321 acttactgaa tttattagcg cctgtgttgc atatattaca tctcttaagt tgaagtgtac
     4381 tatgtcatgc aatgcggtga gctgtttatg cggagcttaa acagatggtg acgtgtaatt
     4441 taacatacaa ctcattgaat ttaaaaggta tttttaggta tcattgtttg tgttgacgga
     4501 ggatgcaaat gtctgaaaag gccaaacgct caggaagaag aaccctggct acaaaaagag
     4561 aaaacctaat tgcaggatta tggggggatg agcttgaaga gttaaatata tggagccgtc
     4621 atgaacatga tggattcact acattaccta gaaccctcac gtatataaac agaatactgg
     4681 attttctagg gggatctggc tctccattat ctcaaaccta ccttgctctt tggtgcagag
     4741 tttttgatga gggatttgtc gaaattcggg ataaagaagc ttttgcgtat gagtcaggat
     4801 ttagcggtca gagagctgtt actacatggt ctgggaggat gaaaaaacta aaagaacttg
     4861 gttttatttt aaccaagcct ggcacgtcgg gagagtttca atatgtgatc atattaaatc
     4921 cgttcccggt aattaaaaac atttacgaga acaaagagaa agatgaacgt tataacgcac
     4981 tagttgggcg tatgcaggag gtcggagcta aatgggaatg attctatggc taactatttt
     5041 ccggatttag ttatatcaga agttaattat gattttagtc acttagatcc attcgtaatg
     5101 gtggtgaaaa gtgatatggc tagaaaggag ttgaaagtac acgttagatt cacaaaccat
     5161 tgttttacta agggatacga tgcttttagc catcctgcag gggagccgat tattcctgac
     5221 catagcggaa acaaaagaac tttttgccca ataagatata ggctctcgtt gagtctgcct
     5281 gcaatcatca gcagcttggt gaatccgcaa gaaaaggttt ttcaaacagc agcaaggcga
     5341 aactgggctt actcaataac gattgatgat cctgcagggc cgtatcattt gttttttgaa
     5401 atacgaaaag cctctcgtgc tgaaaggcat ctacaggatt tgaatgttgt tgttgaaagt
     5461 gcgtatcacg aagaaaaggg gtatggacca cctgatcttc ttggtcgaat aggatttgtg
     5521 gctctttgtg gaagagttta tgagggtaaa ccgctggcta caaaaagat
//
LOCUS       DAZUFB010000194         5530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZUFB010000194 DAZUFB010000000
VERSION     DAZUFB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.194"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..121
                     /gene="hokD"
                     /locus_tag="ACHY3M_004848"
     CDS             <1..121
                     /gene="hokD"
                     /locus_tag="ACHY3M_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4319602.1"
                     /translation="VICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(163..342)
                     /locus_tag="ACHY3M_004849"
     CDS             complement(163..342)
                     /locus_tag="ACHY3M_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319603.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            289..561
                     /locus_tag="ACHY3M_004850"
     CDS             289..561
                     /locus_tag="ACHY3M_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319604.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            563..1618
                     /locus_tag="ACHY3M_004851"
     CDS             563..1618
                     /locus_tag="ACHY3M_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319605.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            1619..1984
                     /locus_tag="ACHY3M_004852"
     CDS             1619..1984
                     /locus_tag="ACHY3M_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319606.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            1993..2523
                     /locus_tag="ACHY3M_004853"
     CDS             1993..2523
                     /locus_tag="ACHY3M_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4319607.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            2765..2962
                     /locus_tag="ACHY3M_004854"
     CDS             2765..2962
                     /locus_tag="ACHY3M_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4319608.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3113..4171
                     /locus_tag="ACHY3M_004855"
     CDS             3113..4171
                     /locus_tag="ACHY3M_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS4319609.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWLLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKTAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            4212..4287
                     /locus_tag="ACHY3M_004856"
     tRNA            4212..4287
                     /locus_tag="ACHY3M_004856"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4245..4247,aa:Ile,seq:cat)
     gene            4368..4444
                     /locus_tag="ACHY3M_004857"
     tRNA            4368..4444
                     /locus_tag="ACHY3M_004857"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4402..4404,aa:Arg,seq:tct)
     gene            4968..>5530
                     /locus_tag="ACHY3M_004858"
     CDS             4968..>5530
                     /locus_tag="ACHY3M_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4319610.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTF"
BASE COUNT         1396 a         1306 c         1550 g         1278 t
ORIGIN      
        1 cgtcatctgt ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt
       61 acgaatccga accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta
      121 agagaccagg cgagggagaa atccctcgcc acctctgatg agtcaggcat cctcaacgca
      181 cccgcactta acccgcttcg gcgggttttg ttttttcctg gcattctggt ttacaattcg
      241 cacgtcagcc tgaacacctg acacctgctg cgccagcaga gaaaacagat ggtgcacaaa
      301 accaaatttc acaattctga taccgacctt gccatccggc atgggcggcg ttcacacgca
      361 tttaaaaccg actggtacca acacccacca tgtactgaag aacaggccga atggctaatt
      421 cataactacc gcagacgcgg atacgagatt aagaaagccc tcagcctcga ttatcgtcac
      481 tggataatct atgtcaggct cccttattcc gaacgcccac cgcgtccgtc ccgcacattc
      541 cagcaacgca tctggaggta acgtgcgggt attacttcga cctgttccgg taccggaact
      601 tgggctggtg gtgctaaaac cgggccgtga atccatgcag gtatttcata accctcgagt
      661 tctggtggag ccggaaccga aaagcatgcg cggtctgccg tccggagtcg ttcctgctgt
      721 tcgccagccg ctggcggagg ataaatcatt actgccattt ttcagcgatg agcgggtgat
      781 tcgtgctgct ggtggtgctg gtgcattgtc tgactggctg ttacgccaca ttaaatcctg
      841 ccagtggcct catggtgact accatcacag tgaaaccgtc atacatcgtt acggtaccgg
      901 cgcgatggtg ttgtgctggc actgcgacaa ccagctgcgt aaccagactt ccgaatcact
      961 cgggcagctt gctcaccaaa acctgtcagc atggatgatt gatgtcatcc gccacgcaat
     1021 gaatggcacg caggagcggg aattatcgct ggctgaatta tcctggtggg caaccataaa
     1081 taacgtagcg gacgcactac cggagacggt gttacgtcgt tcactgggat tacgcgcgga
     1141 aaaaattcgc tcagtatacc gcgagagcga catcgtgccg ggagagcaga ccgccaccag
     1201 catgctgaag cagcgcacaa aaaatattgc gctactgcct cacgcccacc agcaaaaccc
     1261 gccacaggaa aagacggtgg taagcattgc cgttgatccg gagtcaccgg ctcagtatct
     1321 ccagcgccag aaaccacgac gggaagagat gcctgtatac acgcgttggg taaaaacgca
     1381 gaaatgcatg acgtgtggta atcaggcaga tgatccgcat cacatcattg gtcatggact
     1441 gggagggatg ggaacaaagg ctgatgattt gtttgttatt ccgctgtgcc gtaaatgcca
     1501 tagcgaacta cacgccgggg taaaagattt tgaagaaaaa cacggcagcc agctgttgtt
     1561 gctgattcgt tttttaatgc acgcgagaaa ttcgggtgtc ctgaagtgga aagcatgaat
     1621 gactgaacgc atagaatttg ttttgcctta cccgccaacg gtgaacactt actggcgacg
     1681 tcgtggcagc acatattttg tatcaaaagc cggtgagcgt tatcgccgtg atgtggcgct
     1741 tattgttcgc cagcagcggc tgaaattaaa cctgtccgga aggctggcga taaagattat
     1801 tgcagagcca ccggataaac gtcgtcgtga cctggacaat atcctgaaag caccactgga
     1861 tgcgctgacg catgccggac ttctcataga cgacgagcag tttgatgaaa tcaatattgt
     1921 gcgcggtcag cgcgttcctg ggggacggct gggcgtgaag atttacaaaa ttgagagtga
     1981 gtgagcgtaa atatgatata tccggaaatt acaggcaaaa gcggcgaaca tttacgcctg
     2041 aacacgctgg aagcagtctg gatccagggg aaattacgga tgtgggggcg gtggtcgtat
     2101 atcggtgggg gtaaatccgg aaatatgttt aaccggttac tggtttcgaa aaagctgacg
     2161 aaaacagcag ttaatgaggt tttacgcagc atgaagaaat ccgggctgga aaaaccggaa
     2221 cttgaggcat tttttcggga tatgaccaga gggaagcaga agagctggtt gtcacattgt
     2281 acagacacag aggcgttgat tattgatcgc gttatcagtg aggtgcttgg ggaatatccc
     2341 gggctaatca atattctccg gcaaaggtac gaaggacggg gaatgagtaa gagaaaaatg
     2401 gcagaatgtt taaatcgtac tcacccggaa tggtgtttca gcacatgtga gaaacgtatt
     2461 gcaggttggt tagccgtggc tgaacacatg ctttatgtac ctatgcacga ttcatttcga
     2521 taaaaaaagc ttgctttttt acgcagaaac agcttgaatt cctgtaagct tcgcaaagct
     2581 gtatcgcgag gcgaaatgca agttttttcg cacaaggaag ccaccggaag gtggtttttt
     2641 tgtgtccgta atatacagca gcgcaataaa ttcgctggtg gttattaata ccgttctttc
     2701 aggttgctgg ctttttcgac aagagttatt ggtgtgtcac gttaaccgga aaagggaaaa
     2761 agacatgctg aaacagcagg atatgacaga aaccgccaga gtggtgttta atgaattaag
     2821 cgttaccgac ccggcgacag tcggggagat tgcgcagaat acttaccttt cacgcgaacg
     2881 ctgccagtta atactgaccc agctggttat ggcgggtctg gcagactatc agtttggttg
     2941 ttacagacgc cttcagtcct gaaggctttt ttatttgtgg taaatgggcg gctggtgggt
     3001 gtaaggggca cccaccagcc atctgctcat gcgttgggtt cacaagcaaa cctcaggccc
     3061 actgctttgc gcaaaagcag aatgagccta tcagagacag gcttaatgat ccatgcttaa
     3121 tactgtaaaa atatccagtt gtgagttaat caacgccgac tgcctggaat ttatgcggtc
     3181 gttacccgaa aattctgttg acctgatagt cacggacccg ccgtacttca aagtgaaacc
     3241 cgagggctgg gataaccagt gggcgggtga tgaagattac ctgaagtggc tggaccagtg
     3301 tcttgcgcag ttctggcggg tgctgaaacc tgccggaagt ctttacctgt tctgtggcca
     3361 tcgtctggca tctgacaccg aaatcatgat gcgtgagcgg tttaacgtgc tgaaccatat
     3421 catctgggca aagccgtccg gacgctggaa cgggtgcaac aaggaaagcc tgcgggcgta
     3481 tttccccgcc acagagcgca ttctgttcgc agagcattat caggggccgt atcgtccgaa
     3541 agatgccggg tatgaggcga agggtagggc actgaaacag catgtgatgg ccccgctgat
     3601 tgcttacttt cgtgatgcgc gcgctgtcct ggggataacg gcaaaacaga ttgcagatgc
     3661 cacaggaaag aaaaacatgg tgtcgcactg gttcagtgcc ggtcagtggc tgctgccgaa
     3721 cgaaagcgat tatctgaaat tacaggcact gtttgcccgg gtggcagaag agaagcatca
     3781 gcggggtgaa ctggaaaagc cccaccacca gctggtggat acgtatgcct ctctgaaccg
     3841 acagtatgcg gagctgcaga gtgaatataa gcatctgcgg cggtatttcg gtgtgacggt
     3901 gcaggtgccg tacaccgatg tgtggacgta taaaccggtg cagtactatc cagggaaaca
     3961 tccgtgcgaa aaaacggcag aaatgttgca gcagataatc agcgcaagca gtcgtccggg
     4021 agacctggtt gcagatttct tcatggggtc ggggtcgaca gtgaaagcag cgatggcgct
     4081 gggacgtcgt gcaactggcg ttgaactgga gactgaacgt tttgagcaga cggtgcggga
     4141 agtacaggat ttaatcattc gtaacggatg agattgcgga gttaatcatg cgtcgttatt
     4201 attcagcaat cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg
     4261 ctggttcaaa tccagcaagg gccaccaacc gccactagct catcaggaaa gaacgtcacc
     4321 ctgtgcgaga ttcggagtcc ccggtggcgg tccattatcg gtattctgcg ttgttagctc
     4381 agccggacag agcaattgcc ttctaagcaa tcggtcactg gttcgaatcc agtacaacgc
     4441 accacaccac acttatctgc cctgactctc ttttgcgggc tttttattac aggaaagaca
     4501 ccggacagtg aaatgttaaa tgcctcacaa ttcaggcagt tgactgttgc ctgacatgct
     4561 aagcgtttgt taaaaaaatc ctgcatgatg aatccccctg agcggcgggg cataatgaca
     4621 gatgtttggt tgcgtattgt ataggcaagt tgcggattct gtctggtcat tgcagaattc
     4681 accgggaggc acccggcatc atgctgtata cagagattag gcatatatcc aggcttctca
     4741 tcgcaggagc ctttttacat gcaaaaaaaa gcccgagtgg gttcgggcaa cagcatgaga
     4801 tacttgcatt gtcattttta tcgtgtggat tttaaccagg gtttataagg ctgcgcaact
     4861 gcgcggcctt tttcgttttg cgggctgcgg ttctcctctt ttgattctcc ttgtggccgg
     4921 accgtggccc gcaactgttg aggaaaatcc cggaaagggg aggaataatg gcatttaaac
     4981 actatgacgt ggtcagggcg gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac
     5041 tgaaagaggg ctggcagccg tttggtagtc cggtggccat aaccccttat accctgatgc
     5101 aggcgattgc agcagaaggt gatgtggtgg tcagtggtgc aactgagccg gattggtact
     5161 acgtcatcgt actggccggg cagtccaatg caatggctta cggggaaggg cttccgcttc
     5221 cggattcata cgatgctccg gatccgcgca ttaaacagct ggcgcgccgc agtacagtta
     5281 cgccgggtgg ggctgcctgc agatataacg atattattcc ggccgaccac tgcctgcatg
     5341 atgtgcagga tatgagtacg ctgaatcatc cgaaggcaga cctgagcaaa gggcagtacg
     5401 gctgtgtcgg ccaggggtta catattgcca aaaaactgct cccgtatatc ccgaataacg
     5461 cggggatcct gctggtacca tgctgtcgtg gtggttcggc atttacccag ggcgcggagg
     5521 ggacattcag
//
LOCUS       DAZUFB010000195         5477 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZUFB010000195 DAZUFB010000000
VERSION     DAZUFB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.195"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            131..1489
                     /gene="mltD"
                     /locus_tag="ACHY3M_004859"
     CDS             131..1489
                     /gene="mltD"
                     /locus_tag="ACHY3M_004859"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFS4319611.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESSALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1537..2307)
                     /locus_tag="ACHY3M_004860"
     CDS             complement(1537..2307)
                     /locus_tag="ACHY3M_004860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4319612.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2385..3185)
                     /locus_tag="ACHY3M_004861"
     CDS             complement(2385..3185)
                     /locus_tag="ACHY3M_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4319613.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGLALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3426..4340
                     /gene="yafC"
                     /locus_tag="ACHY3M_004862"
     CDS             3426..4340
                     /gene="yafC"
                     /locus_tag="ACHY3M_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFS4319614.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4337..5140)
                     /gene="dkgB"
                     /locus_tag="ACHY3M_004863"
     CDS             complement(4337..5140)
                     /gene="dkgB"
                     /locus_tag="ACHY3M_004863"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFS4319615.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5302..5378)
                     /locus_tag="ACHY3M_004864"
     tRNA            complement(5302..5378)
                     /locus_tag="ACHY3M_004864"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5342..5344),aa:Asp,seq:gtc)
BASE COUNT         1434 a         1320 c         1359 g         1364 t
ORIGIN      
        1 tattgcaaca acctgaagag cgttttgcat ggttaaggtc aaagaaagat aggttctgat
       61 aaaactttct tgtcatcggc tccgttcgcc gttatgatcg gtcgtctttt aagcaactat
      121 tgacacacac atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca
      181 gagtaccggc aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc
      241 agcaaagttt acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg
      301 tgacttgtgg gctttcattg gcgacgagct aaagatggga attccggaaa atgaccggat
      361 tcgcgaacag aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg
      421 ggcagagccg tatatgtact ggatagcagg gcaagttaaa aaacgtaaca tgcctatgga
      481 actggtacta ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa
      541 tgccgcaggc atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac
      601 ccgcaattat gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaatatgat
      661 gcagcgtctg aacaagatgt ttgacggcga ctggcttctg accgtagcgg cttataacag
      721 cggcgaaggt cgagtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga
      781 cttctggtcg ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt
      841 gagtgatatt ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag
      901 cagtgctctg gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat
      961 ggcggggatt tccgtcagca agctgaagac attcaacgct ggcgtgaaag gctccacgct
     1021 gggcgcaagt ggtccgcagt acgtgatggt gccaaagaag catgcagatc aactgcgtga
     1081 atctctggct tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatacgcc
     1141 gcttaacagc cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg
     1201 tctcggcgta agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa
     1261 gccaggccaa agtttgacga ttggcgcagg cagtagcgca cagcgactgg caaacaacag
     1321 cgatagcatt acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca
     1381 cggcgtgaac atcaaagatg tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg
     1441 cgataagctg acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata
     1501 ataaaaaggc accgattccc ccggtgcctt ttttatttat gccgctttat gtgcttctac
     1561 catgatgata tcactggtga aagagccgtc attctgcaag gcaaaatacg ttctcacctc
     1621 tgtcgatgcg ctctgttggt aaatgcgaat agcgtctatt aacgcttctg gcgtacgcat
     1681 tctcacgacc catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat
     1741 cagattggct tcattgatta acgtcaacca ctcaccgctg gcgtagtttc gtacgtgaga
     1801 ggtatcgcgt aatgcttcta ccgtctgtaa ccagatgtcg cgcactgggt gacccggaga
     1861 cattacgtcc atcacaatca gcctaccgcc aggtttcaat atccggttca cttcacgcag
     1921 tgctgcacca acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc
     1981 gttgtcggca aatggcagac tttcggcata accctggcgg gtggtgatat ttttcagttg
     2041 ccggacttcg gcagcttgtg ccacgacatc tagcatgtgg gcagataagt catacgccac
     2101 caccgtgctc acgttttgcg cagcgacaaa gctggcatgc cctgctccgc agcccatatc
     2161 aagtacactt gcaccaggat aatcagccag acgcaccgcc aggcgctgta aatctcgtcc
     2221 ggatgcatgt acggtactgg tcagatattc gctggcttga gagctaaact gcttttctac
     2281 atggtcatgg tgggattgta ttgtcattgc gctgtccttt tgttatttga gtataaaagg
     2341 gcagcgccaa acgttggtct gccctgtggc agacctgaca taccttattt atcaggcttg
     2401 ccgggactga attcaacgag tagcggattg tgatcggaag cgcgcgtaac cagtacagaa
     2461 gcttcgctga cgttcagacc acggtagaaa acaaaatcga gcgggcgacc aaacgcccga
     2521 cggcgctgat catcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat
     2581 aacgcgttca tccttctacg gctccaggca ttgaaatctc ccgccataat gaccgggccg
     2641 ctgtggtgag ctatctgatc accaatagga agtaactgct tactatagac atccacgccc
     2701 agactgaagt tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga
     2761 tagaccgtca ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg
     2821 cagcaataca ctggatgcgc cgccgaaagg gtcattacac cggaaggatg ttgtggcagc
     2881 acgaaagcgg gcacctgatc ggcggcaaga tagttagcgg tcgcaaactg tactaactct
     2941 ggcgttgtct gcgcttcctg caataacacc agatgtgcat ctttgccgta gttctttaat
     3001 accgacaacc attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc
     3061 tcttcagtac ttaacggttc tccaggtggt aatgccaggc cgatgctcgc aaaagacccc
     3121 ggcggtaaga tcctttccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt
     3181 cgcacttttg actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac
     3241 tgttataggg attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag
     3301 tgcttacaca ggtcagaatt ttgctcatta ttgagaacat taaatcggat gacatatcac
     3361 gcatcctgat ggtttaatga caaataaatt caaaatatct cttctatttt gcaacaggag
     3421 caaaaatgaa agccacgtcg gaagaactcg ccatttttgt ttcggtcgta gaaagcggca
     3481 gctttagccg ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcagtga
     3541 aaaagctgga gatgaaactt ggcgttagcc tgcttaatcg gaccacgcga caacttagcc
     3601 tgacggaaga aggccagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcag
     3661 cggcagaatc agaaattatg gagacgcgta atacaccgcg tggactgtta cgcatcgatg
     3721 ccgcaactcc agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct
     3781 atccggaagt tactttatcg ctagtctcct ccgaaacgat tattaatttg atcgaaagaa
     3841 aagtggatgt cgcgatacgc gctggtacgt taacggattc cagcttacgt gccaggccgt
     3901 tatttaacag ttatcgaaaa attatcgcct cccccgatta tatttcccgc tacgggaagc
     3961 cagaaacgat cgacgattta aagcaacatg tttgcctggg attcactgaa cccgcttccc
     4021 tcaatacctg gccgatagcc tgtagtgatg gacaattaca tgaggtgaag tacggtttgt
     4081 catccaatag tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt
     4141 tgtccgacta catgatcgac aaagaaatcg ttcgcggaga attggtggag ttaatggcag
     4201 ataaagtgtt gccagtggaa atgccattca gtgcagtcta ttacagcgac cgtgcggtaa
     4261 gtacgcgcat ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag
     4321 gagctgtcag agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt
     4381 cgttgcaatc cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg
     4441 ccttaagatt actttccagg ttttcacgtt tagtagaaga aggaattact gagtaacctt
     4501 cccccatagc ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcgatac
     4561 gagcaataac ctcatctttc aaggctttcc cataagccag cgtcatatag gaagtaatat
     4621 ggatgccgtg ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt
     4681 caatctggtt agtagcgatg ttttcagcgc caataacagc aatcgccttt tccatcaatg
     4741 ggatcgtgaa gttggaaata ccgatctcac gcgtcagccc ttgttttttg gcttccagca
     4801 gcgcctgcat aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg
     4861 tcagatcaac ataatcggta cgcaattttt gcaggctctc tttcagactt gggatcaatt
     4921 tgtctttgct gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca
     4981 cgccactttc tgcaatcgcc tgacctactg cggcttcgtt atcatagatt tgtgcggtat
     5041 caattgcgcg ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt
     5101 tcagacggaa agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct
     5161 attttcgtta actgaggtga ttatgggctt cagactgttg aggaaaaaga gcgtaaaatg
     5221 cagaggattt ttgcgattct ggcaataata gatatccaga cgaaaaaaac ctcgagcgtt
     5281 aactcgaggc ttcttaataa gtggcggaac ggacgggact cgaacccgcg accccctgcg
     5341 tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc
     5401 ggttttatga ccggcttact gcttaatttg atgcctggca gtttatgaat cacttcgtga
     5461 ttcacccttc gggccgt
//
LOCUS       DAZUFB010000196         5465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZUFB010000196 DAZUFB010000000
VERSION     DAZUFB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.196"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(126..284)
                     /locus_tag="ACHY3M_004865"
     CDS             complement(126..284)
                     /locus_tag="ACHY3M_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4319616.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(944..1135)
                     /locus_tag="ACHY3M_004866"
     CDS             complement(944..1135)
                     /locus_tag="ACHY3M_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFS4319617.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGGDGENL"
     gene            1200..1523
                     /locus_tag="ACHY3M_004867"
     CDS             1200..1523
                     /locus_tag="ACHY3M_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4319618.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHG
                     ARDDS"
     gene            1732..2163
                     /locus_tag="ACHY3M_004868"
                     /pseudo
     CDS             1732..2163
                     /locus_tag="ACHY3M_004868"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2201..2719
                     /locus_tag="ACHY3M_004869"
     CDS             2201..2719
                     /locus_tag="ACHY3M_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFS4319619.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGNRKFLVIPQGKSATVI
                     PYQGKDWVQLTE"
     gene            2726..3001
                     /locus_tag="ACHY3M_004870"
     CDS             2726..3001
                     /locus_tag="ACHY3M_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFS4319620.1"
                     /translation="MTELERHLLNALEQLQQNYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSEQTERLSEQTERLSQLYSENWR"
     gene            3334..3945
                     /locus_tag="ACHY3M_004871"
     CDS             3334..3945
                     /locus_tag="ACHY3M_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HFS4319621.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGSMAKEFDVETKGKRTYS
                     MNNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEK
                     NYLQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLNESQVDNEVASKWIKE
                     LVGSYTKSLDLSGGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
     gene            4011..4487
                     /locus_tag="ACHY3M_004872"
     CDS             4011..4487
                     /locus_tag="ACHY3M_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319622.1"
                     /translation="MNDFKLSSSELPEILAALREARECLVSRSINEDPQLYSALFLYE
                     KNEGLLSLSFEREYSIFINSNLECILRKVSDIKDKKQGKLQKIMNEFVRFKYSSGDSC
                     ADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVIFEREFERLLGEKNGDF"
     gene            4474..5277
                     /locus_tag="ACHY3M_004873"
     CDS             4474..5277
                     /locus_tag="ACHY3M_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319623.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKES
                     EHKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQE
                     WRKSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYK
                     RLQDEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIV
                     SLNQSIDRLTNQGQKFIESHLERFHIVDATSSLYITGDEKERLLLKNIN"
BASE COUNT         1534 a         1050 c         1534 g         1347 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tactgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 gtggctgctg ccagtggcgc tttgtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg tggcctttct cccggccttt gccttttgcg cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      661 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gtcggaagct
      721 gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggcgca cttttcatac
      781 aatctgcact gatgccgcat ggttaagcca gtatacactc cgctatcgct acgtgactgg
      841 ttcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg ctcccggcat
      901 ccgctcacag acaagctgtg accgtctccg ggagctgtat gtgtcagagg ttttcaccgt
      961 cacccccgaa gcgtgcgagg cagctgcggt aaagctcgtc ggcgtggtcg tgaagtgatt
     1021 cacaaatatc ggcctgttca tctgcgtcca gttcgttgag cttctccagc agcgttaatg
     1081 tctggcttct tataaagcgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg
     1141 cctccgtgta aggggggatt tctgttcatg gggtaatgat accgatgaaa cgcgagagga
     1201 tgctcacgat acgggttact gatgatgaac atgcacggtt actggaacgc tgtgagggta
     1261 aacagctggc ggtatggatg cggcggatct gcctgggtga gccggttgcc cgttccggga
     1321 agctgccgac actggcaccg ccgttactgc gccagctggc tgccatcggg aataacctga
     1381 atcagacggc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
     1441 tggccgcact gatggccatc gagagagagt tgcgcagctt acggcaggtc gtcagggaac
     1501 atggagcgcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1561 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtgat
     1621 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtcaggt
     1681 gttctgtcgt tcgcggagaa ggagctgccg ccgggagggc gtgaaaaagt gatggcgagc
     1741 tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggtggag
     1801 caccaggaca agggacggct tgagctgaat tttgtcattc cgaatatgga gctacagacc
     1861 ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc cgagaattga tgcctggcag
     1921 acactggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata
     1981 ctgactctct ctgataacct gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc
     2041 gggatagacg ctctttatca tgtcggggag ataaaaggcc gtcaggatgt gattcaggcg
     2101 ctcacggagg cagggattga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac
     2161 ccgaacggcg ggaggaatat caggctgaaa ggagcatttt atgagcaatc ttttacagac
     2221 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     2281 caacgagttc aggaggctcg gcgaatctgt cagcgaggct gtgacatcaa acgagacgaa
     2341 aatcagagac gctatagcct tgttcacaac gtcgacagag gaatcgctga aaaaacaccg
     2401 ggaaggggtg aaagaggcga tgatgcagca ccggaaggac gtgttaaagc tggcagggaa
     2461 tacgggcgtg atgttactgg ggatagtctt tctcctgttt accgcgagtg gcgggacgct
     2521 ctggtatctt ggcgggcgga tacaggcgaa cctggaagaa atcaggatac aggaagagac
     2581 attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg ggaacaggaa
     2641 atttctggtc atcccgcagg ggaaatcggc aacggtgatt ccctatcagg ggaaggactg
     2701 ggtacaactg acggagtgac acagagtgac ggagctggaa agacatttgc tgaacgcatt
     2761 agagcaactg caacagaact atatgcagcg gctgaacgaa tgggagagcg ccttcgtgga
     2821 attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat
     2881 gcagttgagt cagcaggtgc agcacttgag cgagcagaca gaacgcttga gcgagcagac
     2941 agaacgcttg agcgagcaga cagaacgctt gagccagtta tacagcgaga actggagata
     3001 agagaggaac ggctgataca ggagcgcgaa catgcattat ccctggaacg ggagcgtcag
     3061 ccggaaatac aggaacgcac gctggatggt ccttcgctgg gatggtgaaa ccatgaaaaa
     3121 tggcagcttc agtggattaa gaggggttaa tgtggcctgt accatctggt tgcataggta
     3181 ttcatgcggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg tttgttgcca
     3241 tttttacctg tctgctgccg tgatcgtgct gaacgcgttt tagcggtgcg tgtaatgcga
     3301 cgttatggta aatagatgag ttaggcctaa gtcatgacag ataaaacgac cgatattaaa
     3361 atggctttag aaaacatgat cagatttcgt gacactatag agagattggg aggttctatg
     3421 gcgaaagagt ttgatgttga gaccaaagga aaacgtactt attctatgaa taacgatatg
     3481 agtgatttat ataatgtaat atcacgggct gttgaagcgt ctggtgccga tcattcaatt
     3541 aatgaaaaat tgacaaatgt cttgaaaaga gaattagttg attatgtcag cattgcgcat
     3601 ctaaaaacca aattgtctgt attatatgag tttgaaaaga attatttgca gcttatcgca
     3661 gaatataaag aagaaataaa atttgcttcc tctttgcaag aggatttacg taaagaacgt
     3721 gctaaattct tttctgagac attaaaagag gttcatcaaa ctctaaatga atctcaagtt
     3781 gataatgaag tggcatcaaa atggattaaa gaacttgttg gtagttatac caaaagcttg
     3841 gatctaagcg gaggccttgt tgaagagcat acattagata caattgcttg tattcgcgct
     3901 gaggctaaat taaataaacc atctattgag ccgggcaata actaattatg gccgggggta
     3961 gacatataaa tagtttgctt tatgttcttg tggctatatt agaacgtaaa gtgaatgact
     4021 ttaaattgtc ttcgtcggaa cttcctgaga tattggctgc tttacgagag gcaagggaat
     4081 gccttgtatc gcgcagcatc aatgaagatc ctcagttata ttcggcatta tttttatatg
     4141 aaaaaaatga agggttgcta agtctttcat ttgagagaga atactctatt tttattaact
     4201 ccaatttaga gtgcatattg aggaaagttt ctgatataaa agataaaaag cagggtaaac
     4261 tgcaaaaaat aatgaatgaa tttgtgaggt ttaaatattc aagcggcgat tcttgtgcgg
     4321 atcttttgtt tttgagtggt aaacttgaac agatacgtaa aatatatgaa gtctttgata
     4381 gggatagaaa tagacttgaa tttcttaact atatgatgaa gaaagaacgt gttatttttg
     4441 aaagagagtt tgaacggctt ttaggtgaaa aaaatgggga tttttagtca gattggtagc
     4501 gctttgctta attgtattag taagcctgtt gatgcactct gtgaatgggc taaagaacca
     4561 ttgaaagata gggaatatag aagagaggag gcttcaaagg agtctgaaca taagcgcaac
     4621 atggatgtct tgacggccaa gaaacgtgtt gaacatgaac tcagaatgag tgaattgaat
     4681 gctgaatatg agctgagaga aaaagagaaa actcttgaag ttgatctaga ggttcgtcgc
     4741 gttcgcgaaa tagaaaaagc agtagctgag atccaggaat ggagaaagtc acaggaactt
     4801 gagaggatgg agcgaacaac agcagctatt gcacgttaca aggagcaatt aacgaaatta
     4861 aatatagaag tgattaactc tattgggaac atgcagcttg atttaaagga gcgcgcacag
     4921 aaatttgtct atgagaaagc tgtacaatat aagcgcctgc aagatgaagc aactgaagag
     4981 gctatgaatg atcttataag gattgaggaa aagtttggtg ataatgaaag agctaaagat
     5041 attttaattc gtgcagttga tactaagatg gggaatgtta ttgatacgtc aactcggttt
     5101 ttggaagaat taaatagaga tattgttagt ttaaaccaaa gtattgatag gctgactaat
     5161 caaggacaaa aattcattga aagtcatttg gagcgttttc atattgttga tgcgacatct
     5221 tctttatata ttacaggtga tgagaaagaa cgattgttgc tgaaaaatat taattagtat
     5281 tttgtgagta atagcgatca ttatttttaa tggtcgcatt gtttgttctt gcggttgcta
     5341 atcattagtt tttgtcttgt ggttgaaaac tgagcgctat ggctatgacc tgggagtggg
     5401 agtgaaaaga ttcaaggatc ctcttgtgat cctttttttc tgcgcgtaat ctgctgcctg
     5461 taaac
//
LOCUS       DAZUFB010000197         5337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZUFB010000197 DAZUFB010000000
VERSION     DAZUFB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.197"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..324
                     /locus_tag="ACHY3M_004874"
     CDS             <1..324
                     /locus_tag="ACHY3M_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="HFS4319624.1"
                     /translation="YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWP
                     LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLN
                     IKHYQ"
     gene            341..601
                     /locus_tag="ACHY3M_004875"
                     /pseudo
     CDS             341..601
                     /locus_tag="ACHY3M_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(743..1867)
                     /locus_tag="ACHY3M_004876"
     CDS             complement(743..1867)
                     /locus_tag="ACHY3M_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFS4319625.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2597..2794)
                     /locus_tag="ACHY3M_004877"
     CDS             complement(2597..2794)
                     /locus_tag="ACHY3M_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4319626.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2860..3130)
                     /locus_tag="ACHY3M_004878"
                     /pseudo
     CDS             complement(2860..3130)
                     /locus_tag="ACHY3M_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3435..3623
                     /locus_tag="ACHY3M_004879"
     CDS             3435..3623
                     /locus_tag="ACHY3M_004879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319627.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            3945..4139
                     /locus_tag="ACHY3M_004880"
     CDS             3945..4139
                     /locus_tag="ACHY3M_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319628.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4482..4610
                     /locus_tag="ACHY3M_004881"
     CDS             4482..4610
                     /locus_tag="ACHY3M_004881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319629.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4920..5255)
                     /locus_tag="ACHY3M_004882"
     CDS             complement(4920..5255)
                     /locus_tag="ACHY3M_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319630.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1576 a         1210 c         1131 g         1420 t
ORIGIN      
        1 tacgcgtatg acaggatacg gaggacggtt gtggcgcacg tcttcggtga acgcactctg
       61 gccacactgg agcgtcttct gagcctgctg tcggcctttg aggtcgtggt atggatgacg
      121 gatggctggc cgctgtatga atcacgcctg aagggaaagc tgcacgttat cagcaagcgt
      181 tacactcagc gcattgagcg acataatctg aatctgagac aacatctggc aaggctggga
      241 cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat
      301 ctgaacataa aacactatca gtaagttgga gtcattacct gagaataata cctcctgggt
      361 aagtgagcag atactttcag cgcctccgaa agatgtaaat attcgacttg gagtcggttc
      421 tttagaaggt acaaccgtct cacatgttca gcgattgcat cattcgttaa tcgcagctgg
      481 tttggaaagt aacctcactg tctataccgg tggtcacgat tatgcctggt ggcgcggagc
      541 aattattgat gaattagcaa attataattg caggaaggta tcaaataata actctgtgta
      601 aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt attatgggag actatctcat
      661 ttatttagtg atgtccttca tttatgtgat gaaggacatt atattactca taacatttct
      721 cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt gttttttaat tttctcatca
      781 aaacctctgc aatctctgca ggactgggtt gttcagccat ttcagcggct acctgatctg
      841 aacagaagcg tagtgaatca tcataaagaa ggcgatttac caaatcactg gtcagtccat
      901 gcttgtccgg aataatccca cacccacgct tcgcaacaat ttctgcatta acagagcgat
      961 ctgcaccttc gccaaacaca atctgtggta ttccactata cagcgctgtc agggtattac
     1021 ctgcgccacc gtgatgaata aatccatcag ctccattaag gaatacaccc ataggtatcc
     1081 agtcaaccag acgtacattt gatggtagtt ttcgtaaacc agtacgagca tttattgcaa
     1141 gttgcaaaat gatatcagca tcaacttcat ttgcagaatc cataacccat gaaatcagct
     1201 ccagaccatc aaccattggt tttacagtgc caaggctgat aagtaaacgt ttccgatcag
     1261 aatccctgtc ccaccatgtt tcctttacag cacctccgtt ataaggaata tatctcattg
     1321 agataaccgg ttctccagca tttttaagaa tgctcatact tgggggagca acatcaatcc
     1381 atgctaaatc acatagtgga ccgctgactc catgccgcct gtaagcattg cttaaagaac
     1441 ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc aacagccagc atcactgaag
     1501 gaattctata tttagcagca accaatgggc ctgccggatc aagaggggga tagactatta
     1561 aatctggcct ccattttcct gcaaaatcga tgaggttatc tgccatttca tcgctaaaaa
     1621 aagagaaatg accaataata ttactttttt tcctcaactc ttcctgatgg cgatatccag
     1681 cctctgaatc taaacctgga actgcatcaa aaacaaccag tccagcttct gctgctttat
     1741 tcgcgaattt gccagcgcta ctaattacta catcatgtcc aattacacga aaggcctgag
     1801 ccagagaaat caatgggtat aacaaaccgt acagcggtgg gccaataaaa agaatacgtt
     1861 ttcgcattaa aaaagtccgt cttctgctta cctgtttatt tttctatctc taagaaaaca
     1921 taattaatat tatttttatg tgagcttatg cacaaaaata accaagtata ttatcttctg
     1981 gtagagattc aggatacagt ttgtaggagc tttctttttt gaacaatgat ttgcaggaca
     2041 gttatgctaa actagcggaa tcagtatcac aacaataaat gactgatcat acctgaaatt
     2101 tgaattaaca ggcatcatga atgtcaatag tctaagcggt gactacttat atcgcttgca
     2161 taaatactaa acactgacta gaaaagtatt ggataacccc gtcactaaaa gtaacgcttc
     2221 ggttatttgc tcggaatgta ttaacgccaa gattgttagc cgttacgcct tatatcactg
     2281 gcgctgacaa atgtttcttt tctaatttac tgaaccggcg cagacacgca ggaaagcacg
     2341 ccggcaccgg tgtaatgcca gttctaatca ataagaatgg aatgacgaat cagtcaggaa
     2401 gggataacaa cccgctgctg aacggaaggt atcagcagga agatgaacgg gaatatcgcc
     2461 agcaccaagg gcagggccgg gttccacaat accagcgctg gcattttgca cattgccagc
     2521 gtaccggcat tacaacacgt ttcatgctgc tgccccctag gattcctcga ttcgttgcta
     2581 catccactgc tctacttcac ttccactgct ccacttcact tttgtaccag cggctgctgc
     2641 gccccagttt gatgggttta gggaaatggc cttcactgat caacttgtaa aaccatttgt
     2701 cggtcatacc ggtgaaactg gtgatatagt tcatgtcgat cagggaatta tcggcacgca
     2761 gaaatacgct gtcgtggctt gttgtagtca acatggtcag aatgtcctgt gagatttatg
     2821 aagattgtgc gaatgcggga aatctactga gctgtgcttt cagaactggc ctgttacggg
     2881 ataccaagga ttaccggcgg ggtaacgggc ttccggatca tacacaccac gattatcgcg
     2941 gacaaaatca ctgaacgccc atatcacctc tttaagtatg tcttcgcagt ccggtaaatg
     3001 acgatccagc gccacatccc gggtggtaca gctttgatgc gcccggtgac acaaagcccg
     3061 gattgttcca gacatcctga atcaaacgcc ccagattagg ggcgtcgaaa tatgcctctc
     3121 tgaccattat attccggcgt ataggtagca ggtctgaagt gacaatgcgt cacctgacgt
     3181 taaaagtcac ccaagatgac cccagtgggg gcaacaagta taaaaccgtt ttttgattaa
     3241 gtggtcggat atgaaaaggg gaaggtttat gtgaaaaata cctgtgtttt ctttgaaaac
     3301 acacaacagt atcaggttat ttatgtttat acagtgcgtt ccaaactgac ctgaataatt
     3361 acgcaagttg caacaataaa acatttttgc cgtagtgtga ggtactccgg taaattgctt
     3421 cccaggtgca gacaatgact aacgaagaac tacaggcaat cgcattatcc atgcttgagc
     3481 gccagcgcga aaaagtacac cagcaacgag agtctttgct taacccatct ccattcacca
     3541 ggccaaaacg attggagctt gaagcattct tggacaaata cccacggcgg cttaaatcag
     3601 gcaaaaccag accaccaggc tgatagctca cagtttgttc tccgattcaa taaaacggat
     3661 aagaaccatc agcgcggtag cgtaaggcac tacagcgcat tgctttacga gtaataacaa
     3721 tggatttatc accagaagac gcaaagtttt tacgagaact gcaacaccgt atgctcagca
     3781 ttatcgaaca gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat
     3841 ctcctttagt tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt
     3901 tttcattagc acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa
     3961 taaaaaggct ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac
     4021 gtgccgcgct gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata
     4081 atggcaaaaa aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac
     4141 aacaatctgc atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt
     4201 ctttatatcc actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta
     4261 caagcacact agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata
     4321 cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc
     4381 catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc
     4441 ccaatatcct gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag
     4501 agttctcggt acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg
     4561 aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta
     4621 tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt
     4681 ggcaatatcg aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat
     4741 gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct
     4801 aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct
     4861 gcattatgtt aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac
     4921 tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc
     4981 acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct
     5041 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
     5101 cttttaccag tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt
     5161 atagcattgt ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca
     5221 gtccgttttt cataacccac tgtactgata accataatct aatcagtaga aattgagtcg
     5281 aaaataagca ctactccata caggataatt agaggtcagt ttgattattc acaattc
//
LOCUS       DAZUFB010000198         5336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZUFB010000198 DAZUFB010000000
VERSION     DAZUFB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.198"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            238..411
                     /locus_tag="ACHY3M_004883"
     CDS             238..411
                     /locus_tag="ACHY3M_004883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319631.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            412..876
                     /locus_tag="ACHY3M_004884"
                     /pseudo
     CDS             412..876
                     /locus_tag="ACHY3M_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
     gene            972..1532
                     /locus_tag="ACHY3M_004885"
     CDS             972..1532
                     /locus_tag="ACHY3M_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4319632.1"
                     /translation="MISVMILSVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYFSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            1668..2459
                     /locus_tag="ACHY3M_004886"
                     /pseudo
     CDS             1668..2459
                     /locus_tag="ACHY3M_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(2464..3834)
                     /locus_tag="ACHY3M_004887"
                     /pseudo
     CDS             complement(2464..3834)
                     /locus_tag="ACHY3M_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3854..4201)
                     /gene="tnpB"
                     /locus_tag="ACHY3M_004888"
     CDS             complement(3854..4201)
                     /gene="tnpB"
                     /locus_tag="ACHY3M_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4319633.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(4198..4872)
                     /gene="tnpA"
                     /locus_tag="ACHY3M_004889"
                     /pseudo
     CDS             complement(4198..4872)
                     /gene="tnpA"
                     /locus_tag="ACHY3M_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            5126..5336
                     /locus_tag="ACHY3M_004890"
                     /pseudo
     CDS             5126..5336
                     /locus_tag="ACHY3M_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1290 a         1209 c         1447 g         1390 t
ORIGIN      
        1 accgagacgg gcatgtccag actgctcgtt agccagtgaa taacaatcag aaagtaccat
       61 aacagacccg aagaaaatga aatataagag aagatcaacg ggtgaagaaa aagttcaaaa
      121 aatcgctgcc ggggaggaag gaaactaccg gatggaaaga gtccccctaa agcagactga
      181 cagacatcac aaatccccgg gtggggattt gtgtataaga gacaggttac aggtgtggtg
      241 aataagactg caaatgtggc agcccatgcc gtggtgaatg ctgcactggc ggtggtacag
      301 ggaaataacg cccgtgccag tgccgcaggt gctgccactg gagacgtggt ggggatgata
      361 gccacagaga tgtgcggaat atcccctggc actgttacat gtaaaagata aataacgaag
      421 ctacatcctt ctctttatga acggctttat attagttttg ctgacaagat tgagctaaat
      481 ccggccaatg aagaagagca ggttattctt taaaggtttg ataacatgta acacattttg
      541 aatattcagt cagtaagcct taaaaattta cctggttatg ggctgcctga tataacaaag
      601 taaatattcg atggggaatg ccggatgcaa tgaacaatgc attaacgtat cgaaataatg
      661 atgcaggagc aaactcagtc tggtgagtat acattatgcc attgatgcag agtttaaagg
      721 aacagggctg gttcgttatg gaacaacatt tgtgcctgag gaaagggggg ggagtatctg
      781 aggcaacaga agtttgtggg gagttcggtt tattaccatg cttggttttt tgtcagtgtg
      841 ttcaaatcgt caggtgatat atttatagtt ttttgatgat gatttcaaat aatgtttttg
      901 agatgtgtgg tcctttttga ttttcagaaa tcatttatta atgaaggagg gttaaatgaa
      961 aaaaactcgt tataatttca gtaatgatat tatctgttat gagtggttca agactggctg
     1021 ataatcatac gctatcggct ggttatgctc agagtaaggt acaggacttt aagaatatta
     1081 aaggagtgaa tctgcaatat cgatatgagt gggattctcc ggtcagtgtc gtcgggagtt
     1141 tttcgtatat gaaaggtgat tgggcggaca gtcatcgcga tgaagctgac gatttttaca
     1201 gacatcaggc tgatattaaa tatttttcat ttctcgcagg cccagcttat cgtctgaatg
     1261 actatatcag tttttatggt ttggtgggga tatctcatac taaagcgaag ggagattatg
     1321 agtggagaaa cagtgtgggt gctgatgagt ctgatggata tttaagtgag tctgtatcaa
     1381 aaaaatccac tgattttgct tatgcagctg gtgttattat taatccgtgg ggcaatatgt
     1441 ctgtaaatgt tgggtatgag ggaactaaag cagatatata tggaaaacat tcggtcaatg
     1501 gatttactgt tggagttggt tatcgttttt aatcattaga cgggactgga taattgcact
     1561 tgcgtagtaa agtttactaa atgtttgtat tttaatgaag tattcgacat attcccttca
     1621 gacggaatat gtcgacggtt atggttttac tggagcatga gtatgttatg gcgaaactga
     1681 taaatatatc agcgtccact gaggagagaa tgacgccagg ggagaggagg gtggcttcac
     1741 gactggagag ctttctgaat gatgattgct ttgtctggta cgatatcccg gtgggacgga
     1801 aaaaccgaca tccggatttt gtcattattg acccggataa cgggctggta tttctggagg
     1861 tcaaggactg gactgtatcc acattacgta aagcaaatca ggagcaggtt acactggaaa
     1921 ccgacggtct tctgaaatca gaaataaatc cgctggtaca ggttcgtcgt tatgcctgtg
     1981 atacggttaa tgccttacct gctgacccgt gcttacggca gaatgacggt cagtataagg
     2041 ggcggctgaa cctggcctgg gcgtatggtg tggtgtttac ccgcattacc cgtcagcaac
     2101 tgaaggcact gacagggaat aatgagaatg cggtggaaaa gatttttcct tcagcacaga
     2161 caatctgtca ggatgaaatg acgcagtctg ttttacctga ggtattccgg caaaaaattg
     2221 ccgggatgtt taccacgggg ttcagaaccc gggtaacacc ccggatgagg gatattctgc
     2281 gcgcccatct gtttcctgaa gtgaccgtga aacagaactc acaaatcaaa attatggata
     2341 ttcagcagga gattctggcc agaaatattg gtgacgggca tcgtgtcatt catggtgtgg
     2401 ccggttccgg aaaaaccatg attcttttgt tccgttgcct ttacctggca gaaaccacgt
     2461 ccggtagtta cgccgcccca gtgcaactac ccggagggcg ttttcacaca ggttattgtc
     2521 gatctccacc cagccattgc ggcagtattc gttcagagcg tcccattgct tcagcagata
     2581 ggtgaacgct ttcgctgtat ccgagtggcg cgacagcgtg ttcatctgcc cctggagcca
     2641 ctcatacaac gactgcatta gcggtaccgt tctggctttt ctgaccgcca gtcgctcttc
     2701 tgccggactg ccgcggatct cagcctcgat agcgtacagt tccccgatac gacggagcgc
     2761 ttctcccgtt actgtcgttg gatggcggac atgtacatcg tggatcttgc gtcgggcatg
     2821 tgccatacaa gccgcttcgg ttgcctgacc gctttcgtaa agagcattgt aacccgcata
     2881 tgcatcggcc tgcaggatac ctctgtagtc cgccagatgt tgctgtgggt ggatgccttt
     2941 gcggtcggga gagtatgaga accacaccgc tgccggcatg gtcgaaccgg cgttgcgatc
     3001 gtcacgaaca tagacccaca gccgtccggt acgggtttta ccctgacccg gctccagtac
     3061 atttaccggc gtgtcatcgg catgcacttt acccggcatc agcacatagt gcttcagttc
     3121 atcatacagc gggcgaagct gctctcccat gatgtcaacc cagcgcccca tcgtattgcg
     3181 gtgcagctcc acgccctggc gggcatagat ttccgactga cggtacagcg gcagatgctc
     3241 ggcgaactta gccatgatta tgcgggccag cagagccgga ctggcgtaac tgcgctcgat
     3301 gggttttggt ggctgcggag cctgaactat acagtcgcac cggctgcagg cccgttttgg
     3361 gcgaaccgtt tcgattaccc tgaacgcggt gttgatgata tccagttgtt cagagatgct
     3421 ttctcccagc ggtttcagtt tgccgctgca gacggggcat tcggtttctg ccggggagat
     3481 aacctgcctg tcacggggaa gtgttgccgg aagtgctttg cggacgggag agtctgatgt
     3541 tttcggcgct gtctctccgg ccattgaggt gagttgcaac tgcgcctcac caagcctgtt
     3601 ctggagctcg gttatacgcg tttctgcccg tgcgatcttc ttttctatct tctcgcggct
     3661 tttctcgctg ctgcgaccga acaacattct ctgcagttta gcgaccagcg ctctgagtga
     3721 gctgatctcg cgggcatagc cggttatttc accagacaga cggacgataa cagcctgctg
     3781 tgcgatcagc agggccttca gttgctcgat gtcgtcgggg agtgtgttgt tcattcccct
     3841 gttttatcac gggttatatc cggatgccag gccgttctgt ccgtttggga tgttgccatg
     3901 cgatcccctc cagtagcatg gataactgag ctggcgtcag gtgcactttc ccttcccggg
     3961 ttaccggcca gacgaagcgg ccccgttcca ggcgtttggc gaacaggcat aacccgtcac
     4021 gatcggccca cagtattttc accattttgc cactgcggcc ccggaagacg aagatatgcc
     4081 cggagaacgg ggcatctttc agcgtgttct gcaccttcga agccaggccg ttgaagccac
     4141 aacgcatatc tgtgatgcca gcgatgatcc agattctggt accggttggc agcgttatca
     4201 tcgggtacct cctttcattt cgcggattag cgcccgtaac atttccggag tgagagggtc
     4261 aaacagtttt accacacctg atttaagatg cagctcgcac cgtgggacgt ttccgggatc
     4321 actctcaggg cactcatcag gcttgttacg ccagaaggga tttgtaactg gtctggtcgg
     4381 ctctggcgta tcagtcagag ccaccgggac aggcatgcat tcctgtatgt catcatcgct
     4441 cagtaagccg tcctcgtact ggcttttcca tttaaacagc aggttatcat tgataccgtg
     4501 ctctctggcg atccgggcaa caacagcacc gggctgtaat gcctacttag ccagacggac
     4561 cttaaattca cggctgtagc tggctcgccg ttcttttcgc catgtgcctt cgctgatttg
     4621 aggctctgtt aattccttct ttctgttggc ataaaggatg gcgtcaagct gagctaatga
     4681 aactgaatcg ggcaatggcc atgcgatacc ggatgcaata aatcgctgaa aaagcgtatg
     4741 tattgtggaa tgactgagac ctagacgctg agcgatggcc cggatggtca gtttatcttc
     4801 aaatcttaaa cgcagagcat caggcaaata agaacggaag cagggaatat ctttttttgt
     4861 ctgggaattc atcgttcgtg tccatctata tagatgggcg cgattgttgc cagacaggac
     4921 aattttcaca agacgtcgca gatggggcgc ttacacaaca atggcgcgag ggaaagctgc
     4981 tattaccttc ttcagagagc ccccagctac ttcctgtgac tctcgatgca gctgccgaac
     5041 agccagaatc gctcgcagag gacccggaaa ccctcagtat cagctgttgt caacgacgga
     5101 tgactattgc accgttcgtg acagtgtgct gcaagcggat gcgtacgccg ggttcaacga
     5161 gctgtatcgc aatggtggga taacggaagc tgcctgctgg gctcatgccc gccgaaagat
     5221 ccacgatgtg cacgtccgca tcccgtcagc actgacggaa gaagccctgg agcagatcgg
     5281 tcagttgtac gccatagagg cggatataag gggaatgccg gcagagcagc ggcttg
//
LOCUS       DAZUFB010000199         5276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZUFB010000199 DAZUFB010000000
VERSION     DAZUFB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.199"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..431
                     /locus_tag="ACHY3M_004891"
     CDS             6..431
                     /locus_tag="ACHY3M_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4319634.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            428..778
                     /gene="tnpB"
                     /locus_tag="ACHY3M_004892"
     CDS             428..778
                     /gene="tnpB"
                     /locus_tag="ACHY3M_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4319635.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            809..2422
                     /locus_tag="ACHY3M_004893"
     CDS             809..2422
                     /locus_tag="ACHY3M_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc47 family transposase"
                     /protein_id="HFS4319636.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKLHTDDTPVKVLA
                     PGLKKTKTGRIWTYVRDDRNAGSSSPPAVWFAYSSNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGSALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAARQQRSKALLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEVDNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLSGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(2628..2704)
                     /locus_tag="ACHY3M_004894"
     tRNA            complement(2628..2704)
                     /locus_tag="ACHY3M_004894"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2668..2670),aa:Arg,seq:tct)
     gene            complement(2718..2794)
                     /locus_tag="ACHY3M_004895"
     tRNA            complement(2718..2794)
                     /locus_tag="ACHY3M_004895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2758..2760),aa:Arg,seq:tcg)
     gene            complement(2804..2879)
                     /locus_tag="ACHY3M_004896"
     tRNA            complement(2804..2879)
                     /locus_tag="ACHY3M_004896"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2844..2846),aa:Ile,seq:cat)
     gene            complement(2908..3621)
                     /locus_tag="ACHY3M_004897"
     CDS             complement(2908..3621)
                     /locus_tag="ACHY3M_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319637.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(3756..3953)
                     /locus_tag="ACHY3M_004898"
     CDS             complement(3756..3953)
                     /locus_tag="ACHY3M_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4319638.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(4177..4731)
                     /locus_tag="ACHY3M_004899"
     CDS             complement(4177..4731)
                     /locus_tag="ACHY3M_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4319639.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIDKKVIAF"
     gene            complement(4740..5099)
                     /locus_tag="ACHY3M_004900"
     CDS             complement(4740..5099)
                     /locus_tag="ACHY3M_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319640.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(5112..>5276)
                     /locus_tag="ACHY3M_004901"
     CDS             complement(5112..>5276)
                     /locus_tag="ACHY3M_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319641.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT         1388 a         1373 c         1279 g         1236 t
ORIGIN      
        1 taagtatgga attacaggac tggcgaaaag aacctcgtaa aaactattcg aatgaattca
       61 aacttcgtat ggtggaactg gcatcacaac ctggagcttg tgttgcacag attgcacgtg
      121 aaaatggcgt caatgataat gttattttca aatggctcag gctctggcag aacgaagggc
      181 gtgtttcgcg gcgtcttccg gtaacgacct cttctgacac tggcgttgaa ttattacctg
      241 tagaaataac gccggatgag cagaaagaac ctgtggcggc cattgcgccg tctttatcca
      301 cttccactca gaccagagtc agtgccagtt cctgcaaggt ggaattccgt cacggtaaca
      361 tgacgctgga aaatccatcg ccagagctgc tcacggtgtt gatccgtgaa ctgaccggga
      421 ggggaagatg atctcactcc catcaggtac ccgtatctgg ctcgttgccg gcgttaccga
      481 tatgcgtaaa tccttcaacg gactgggaga acaggtacaa catgtgctga atgataatcc
      541 cttctccggt cacctgttta tcttccgtgg ccgacggggt gacaccgtca aaattctttg
      601 ggctgatgct gatggtctgt gcctgttcac caaacgcctg gaggaaggcc agtttatctg
      661 gcctgcggta cgtgacggca aggtatccat tacccgctcg caactggcaa tgctcctcga
      721 taagctggac tggcgtcagc caaaaacatc cagccgtaac tcactgacaa tgttgtaaaa
      781 aactcctgac cgcattataa aaacggtcat gagtcagaaa tacctcattc gcatcgcaga
      841 gctggaaagg ttgctctctg agcaggctga agccctccgt cagaaagacc agcaactgag
      901 tctggttgaa gagacggaag ccttcctgcg ctctgcactg acacgtgccg aagaaaagat
      961 cgaagaagat gaacgggaaa tagaacatct gcgggctcag atagaaaaac tgcgccggat
     1021 gctgttcggt acccgttctg aaaaactgcg tcgtgaagtt gaactggctg aggctctgct
     1081 gaaacaacgt gaacaggaca gcgatcgtta cagtgggcgg gaagacgatc ctcaggttcc
     1141 ccgccagttg cgacagtcgc gccatcgtcg tccgttaccg gcacaccttc cccgtgaaat
     1201 acaccgcctg gagccagaag aaagctgttg cccggagtgt ggcggtgagc tggattatct
     1261 gggagaagtc agcgctgaac agctggaact ggtgagcagt gccctgaaag tgatccgcac
     1321 agaacgggta aaaaaagcct gtacaaaatg tgactgtatt gttgaagcac cggcgccgtc
     1381 ccgcccgata gagcgtggta tcgcgggccc cggattactt gcccgcgtgt taacgggaaa
     1441 atactgcgaa catctgccac tgtatcgtca gagtgaaatc tttgcccgcc agggtgtcga
     1501 actgagccgg gccttactct ccaactgggt tgacgcgtgc tgccagttaa tgacaccggt
     1561 gaatgatgcc ctgtaccgtt atgtgatgaa tacccgcaag cttcacactg acgacacacc
     1621 ggtaaaggta ctggcaccgg gcctgaaaaa gacgaaaaca gggcgcatct ggacgtatgt
     1681 ccgggatgat cgcaatgcgg gttcgtcatc tcctccggcg gtctggttcg cgtactcatc
     1741 gaaccggcag gggaaacacc cggagcaaca cctccgcccc ttccggggta tcctgcaggc
     1801 ggatgcgttc acaggttatg acaggctgtt cagtgcagaa cgtgaaggta gtgcgctgac
     1861 agaagttgcg tgctgggctc atgcccggag aaaaatccac gatgtataca tcagcagcaa
     1921 aagtgcgacg gcagaagaag ccctgaagcg aatcagtgaa ctgtacgcca tcgaggatga
     1981 aatacgggga ttaccagaat cagagcgtct tgcagccagg cagcagcgaa gcaaagcgtt
     2041 actgacgtcg ctgcatgaat ggatggtgga gaagaatggc acgctgtcga aaaaatccag
     2101 actgggcgaa gcgttcagct atgtactgaa tcagtgggac gccctctgtt attacagtga
     2161 tgacggtctg gcggaggtgg acaataacac agcggaaaga gcgcttcgtg cagtctgtct
     2221 cggaaagaaa aattacgtgt tcttcggtag cgatcacggc ggcgagcgtg gagcactgct
     2281 gtacgggctg agcggcacct gccgtctgaa cggtatcgat ccggaagcgt atctgcgcca
     2341 tattctgagc gtactgccgg aatggccctc caaccgtgtt gacgaactcc tgccatggaa
     2401 cgtagtactc accaataaat aagcgtcaat acggtgctcc gttgacgctt acaaacatcg
     2461 tgacgttcct ttgggtgggt gagccaacgc ccgggagcga tctgcccaca gagaaagtca
     2521 cactgaccac tccgtaagct cacccccgaa aggctctgtg gttgatatgc gccgggcgtg
     2581 gcgcagatac aaaaaaggcc cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag
     2641 tggagtctaa ccactgaccg attgcttaga aggcaattgc tctgtccgac tgagctaaca
     2701 acgcatgatg ctgataatgg accgccatcg gggacttgaa ccccgcgcag ccagcttcga
     2761 aggctggcgc tctgtcccga tgagctaata gcggtatgta atatggtggc ccttgctgga
     2821 tttgaaccag cgacctggcg attatgagtc gctcgctctc accactgagc taaaggaccg
     2881 agccaaaaaa taataatcag atgaaaatca ataatcaagc ccttgcctgg atacatatct
     2941 gtctggcggg aagccataat agcggtgaaa tacagaaata aagtaggacc tgcttgaata
     3001 accgcatttt tctgctatag cctgtccata tccatgccgg gaacataaca tatttacagc
     3061 aacacgcatc cgctcttcca gcaacaagcg actgaacatg cccccttcat ttttcagttt
     3121 tgtctttaac aaactctcac tcatatgcaa ctgtagagca atcgcaccaa gcgtccagct
     3181 tgctgatata tctgtctgaa ttatcgccct gactttggca cttatgctgg ataaacatcc
     3241 acttaaaaac aatgacatcc gttcatctgt ttcaaacaga gacaggcagg ccatcataag
     3301 aaacatatcc gtgacctctc cggaaaatcc ctggctggta attaaagccg cagccaacgc
     3361 aggattgttg ggttccagca acaggtaaag cggaatgtca gtcagacgag tcctcgtcag
     3421 cttatgctga ctttccagat attgacttac gacggattcg cttatatcga caattttaac
     3481 tttgccataa tgcataagga aaagctccct gatgcatttg gtggccagaa cgactgagcc
     3541 tggcttaagt gacaacgtat ccttttcaag aaaaatatta attggggagc aaaccatgat
     3601 aactgaacag acaacagcca ttataatttt actttcatta gcaattggtt agctcaatta
     3661 tagccccaaa aggtaaatta tcatcaacac ataagcaaag gactgacagg tgtcgccccc
     3721 ccaccagccg cccattcacc acaaataaaa agccttcagg actgaaggcg tctgtaacaa
     3781 ccgcactgat agtctgccag acccgccata acaagctggg tcagtattaa ctggcagcgt
     3841 tcgcgtgaaa ggtaagtatt ctgcgcaatc tccccgactg tcgccggttc ggtaacgctt
     3901 aattcattaa acaccactct ggcggtttct gtcatatcct gctgttttag catgtctttt
     3961 ttcccttttg gttaacgtga cataccaata actcttgtcc aaaaagccag caagcagaaa
     4021 gaccggtatt cgcaaccacc agcgcattta acgccccgtg ccgtttttca gtcataaaaa
     4081 aacccgcaaa aagcgggctc tttcaaatgt ccatgtctgc tattcgcctc gcggtacagc
     4141 tttgcgaagc gtaccggaat tgaagcagtt tttacgtcaa aaagcaataa cttttttatc
     4201 tataccaaaa gccataacca ttggtttgta caaaataaat tctgccacct ttagccaatg
     4261 ctcaatgcgt ctttcacagg ttcttaaact ccattccgga tgtgcatcat tcagcagttc
     4321 agccattttg cgcttagtca tcccccgccc cacataacgc tgactcagaa cattgagcag
     4381 cccgggataa cctgccagta cttcaccaat aaccctgtcg attattaacg cctctgaatc
     4441 ggtacaatgt gccagccagc ttttttgatt gccgttgatc atatcccgca aaaaagcctc
     4501 aagttcaggt ttgtccagac ccgctttttt catcctccgg agcgcctcgt taattgccgt
     4561 ttttgtcagc tttttagagg tcagtaattg gttgaacata tttcccgtct taccgtcgcc
     4621 aatatacgac caacgccccc acatacgcag tttcccctgg atccagacac tttccagcgt
     4681 tttcaggcgt aaatgctcac cgcttttgcc tgtaatttcc gggtatatca tatttatgct
     4741 cactcacttt caattttgta aatcttcacg cccagccgcc caccagaaac gagctggccg
     4801 cgcacaatat tgatttcatc aaactgctcg tcgtctatga gaagtccggc atgcgtcagc
     4861 gcatccagtg gtgctttcag gatattgtcc aggtcacgac ggcgcttatc cggtggctct
     4921 gcaataatct ttatcgccag ccttccggac aggtttaatt tcagccgctg ctggcgaaca
     4981 ataagtgcca catcccggcg ataacgctca ccggcttttg atacaaaata tgtgctgcca
     5041 cgacgtcgcc agtaggtgtt caccgtcggc gggtaaggca aaacaaactc tatacgcatc
     5101 agtaacctct tttacccgag cacgccggtt gcaaaggcgt gatcaagaaa acgaaaaatt
     5161 aaatcaacct gggaaccatg cttttcttcg aacgccagcg gatccgcatg aagctcgttg
     5221 tgatgctccc gacacagcgg tagcgtgaaa atatcgtggg attttgttcc cattcc
//
LOCUS       DAZUFB010000200         5265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZUFB010000200 DAZUFB010000000
VERSION     DAZUFB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.200"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(149..1168)
                     /gene="rspB"
                     /locus_tag="ACHY3M_004902"
     CDS             complement(149..1168)
                     /gene="rspB"
                     /locus_tag="ACHY3M_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFS4319642.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1180..2394)
                     /gene="rspA"
                     /locus_tag="ACHY3M_004903"
     CDS             complement(1180..2394)
                     /gene="rspA"
                     /locus_tag="ACHY3M_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFS4319643.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2600..2926)
                     /gene="ynfA"
                     /locus_tag="ACHY3M_004904"
     CDS             complement(2600..2926)
                     /gene="ynfA"
                     /locus_tag="ACHY3M_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFS4319644.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3061..3402
                     /gene="ynfB"
                     /locus_tag="ACHY3M_004905"
     CDS             3061..3402
                     /gene="ynfB"
                     /locus_tag="ACHY3M_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFS4319645.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3437..3997
                     /gene="speG"
                     /locus_tag="ACHY3M_004906"
     CDS             3437..3997
                     /gene="speG"
                     /locus_tag="ACHY3M_004906"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFS4319646.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(4000..4710)
                     /gene="ynfC"
                     /locus_tag="ACHY3M_004907"
     CDS             complement(4000..4710)
                     /gene="ynfC"
                     /locus_tag="ACHY3M_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFS4319647.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4818..5123
                     /gene="ynfD"
                     /locus_tag="ACHY3M_004908"
     CDS             4818..5123
                     /gene="ynfD"
                     /locus_tag="ACHY3M_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFS4319648.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1438 a         1333 c         1138 g         1356 t
ORIGIN      
        1 aatccataaa ttcaagcgca gtgcccagcc atcccgatac tgctgctttc accaaatcct
       61 tagtgcttct ttcatgtttt tctattgtca taatggttat ctctaaaaaa gaggtaagat
      121 gcgtactact tactcgccgt tattggtatt attcagaaaa agtgagcaag actttgcagc
      181 aatgtttttg atcctgttca aataaactaa tggcatcagc aacatgctgg aaatcaaacg
      241 tatgggtaat taatttttct ggtttaatta accctttact taaccagtcg ataacaaccg
      301 gaaatttatt tgcatttaag cgtgaagaga aaatagagag ttcttttccg gtaattcctt
      361 gctgaatcac ttcagacggt tcactggaga agcccatcaa tacaatacgt gccgctggag
      421 aagccagcgt tacggcctct ttcaggatag aaggatgaca agccgcatcg ataattaatg
      481 tcggcttgat gcctttttca gcgaaaatct cgccaagcgg tgtctggctg ttattaatcg
      541 cccagtctgc cccgctctct ttcgcttttt ccagtcgttc atcaatgcga tcggcaacaa
      601 tcacattttt aacgttatag acgcctttta atacctgaac gatcgtcagg ccgattggac
      661 cggcaccata aaccagaacg gtatcatttt cagtcggttg accatgaccg gtaacgttag
      721 ccgcaatggt aaaaggttcg atcatcaccg catattgatc ggccactgct tcaggaattt
      781 tccacgcatt ttttgccgga accacggcat attcactgaa accaccgtca gcgtgcacac
      841 ctaatacagc aagtgtcgta caaacgttcg gcttacctat agagcacgga tagcaatgcc
      901 cacagctgac caccggatcg acagcaacgc gttcaccgac tctggcgctt tccacacctt
      961 cacccaccgc atcaatgacg ccaaagaatt catgaccaat gacgcgcgga tatttcgcaa
     1021 aaggattatg cccacggtaa atatggctat ctgaaccaca aattccggca agtttcactt
     1081 ttactcgtac ttcacccgct gacggggtgg gtatttcacg ttcgataatc gacagttgat
     1141 tcggtttttc aattaaaatg cttttcatta ccttactcct taccagttcc acagcgtgcc
     1201 atcttccaga cgtgcgactg gcagataagc aggttcatag ggatatttcg ccgccagctt
     1261 ttcatcgaat tcgatgccaa gacccggttt gtctcccgga tgcatatagc cgttatcgaa
     1321 agtccagttg tgcgggaaga cttcgagcat ttgttcggaa taacccatgt actcctggac
     1381 accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa
     1441 atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat cggcaatccg
     1501 gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg cggatataat cgatgagttg
     1561 ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca ctgcgatggg
     1621 tgtgacggta tgttggcgaa tgagacggaa gcattcctgg ttttccgcag gcgtcgggtc
     1681 ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg cttcaatagg
     1741 cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact tgttacgtac
     1801 cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc
     1861 cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg acatgccgta
     1921 ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc ccagctcctg
     1981 atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat ggcaataaac
     2041 catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt tggcagcttt
     2101 ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg taaccggacc
     2161 gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc
     2221 gcgaccaata agctgcggac aaaggtgatc ctgcaaataa gaggccacgg aaagctcacg
     2281 tccattgagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga tttttaatgt
     2341 gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct tcatgttcga
     2401 ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact accatacaag
     2461 tataaagatc gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat
     2521 ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctct tatcttattg
     2581 ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc aacatgccgc
     2641 aaagcgcaat caacgcaccc gtccagtcat aaagagtcag tttcacgcca tccacaacgc
     2701 gcagccacat caacgccgtg cagacataaa cgccaccata agccgcgtaa acacgcccac
     2761 tcgccgctgg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc cccgccggaa
     2821 gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag catccaataa
     2881 tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt gtctcttatt
     2941 gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacaccc tcctggtacg
     3001 ttttgcgtcc gcagtggatg gtgtagaatc acctttaatc attcatacag ggaatgaatt
     3061 atgaaaatca ctctcagcaa acgaatcggc ctgctcgcta ttctgctgcc ttgcgcactg
     3121 gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc tggcgacagt
     3181 gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac gcgcaatctg
     3241 cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc cgcttttgat
     3301 aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc caatactttg
     3361 cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat tgatttaagg
     3421 aatgtaagga cacgttatgc caagcgccca cagtgttaag ctacgcccgc tggagcgtga
     3481 agatttacgc tatgtacatc aactcgacaa caacgccagt gtgatgcgtt actggtttga
     3541 ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcata ttcacgatca
     3601 gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt
     3661 ggaaattaac catgttcatc gccgcgcaga atttcagata attatctccc cggagtatca
     3721 ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct ttaccgttct
     3781 caatctctat aagctgtatc tgatcgttga taaagagaat gaaaaagcga ttcacattta
     3841 ccgcaagctt ggcttttcgg ttgaaggtga attgatgcac gagttcttta ttaatggtca
     3901 atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggcag agcacaaaac
     3961 accgggtcag actctcctga agccgaccgc acaatagcat taataataat cgatcgtatt
     4021 tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgattagctg
     4081 acagtccacc ggattagcgt gactgtcata ttcacagctc tgttttacat taccaacccg
     4141 ttgattattc agtaaagtaa ccgctgtgta atctaatttt ttgatcggat ccgttgatgg
     4201 cgtggcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca gcggataacc
     4261 ctgatcatca tagcgatatt ccatctgcat ttgtttgctg ctggctttaa tcacgaagcc
     4321 attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct ggcattttcc
     4381 ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg taataattgg cgtccagtac
     4441 cagagcgacc acggtattat tttccagatc cagtaattcg agtgaatcaa aacagccttc
     4501 ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg
     4561 agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag agaaactggc
     4621 catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga ggaatatcgc
     4681 gagtaagcat ggtagtaatt tgtatttcac aacagtcacc agagagtaga gatgattctc
     4741 aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt
     4801 cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg
     4861 cctcacccgt ggtactcgcc gcgccgggat cctgtgagcg catacagagc gatatatcac
     4921 agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg
     4981 accaggttga tcagcctgat tcccaggtcg tcggccattg cgctaatgat acgcataaaa
     5041 ttctctatac ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct acccaggatg
     5101 gcgcgcctgc cgaaccgcag taatacatta tcgactgaac gccggatacg acacatccgg
     5161 cgatttgaac atacaacata atcccacctt attactcata cccttctatt gatatggatt
     5221 aataattctt aacccaaaat gggtagactc cctctattgt tagcg
//
LOCUS       DAZUFB010000201         5154 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZUFB010000201 DAZUFB010000000
VERSION     DAZUFB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.201"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..776)
                     /gene="wzzB"
                     /locus_tag="ACHY3M_004909"
     CDS             complement(<1..776)
                     /gene="wzzB"
                     /locus_tag="ACHY3M_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFS4319649.1"
                     /translation="MSISDNNMSGRSHDPEQIDLIDLLMQLWRGKVTIIICIVVAIAL
                     AVGYLAVAKEKWTSIAIVTQPDAGQLASYNNAMDVLFGANAPEMTDVQAGFIGRFSSA
                     FSALAETLDNQQEPEKLSIDAAVKGQTLPLKVSYTGKTSEEAQKTLAQYIQQVDEGVA
                     KELNADLSMSMETRLADLQKSLAAQEAVAKEQKTLRIAQMTQALKVAQQSNIKLPQVQ
                     QVDQVSQDSMFMLGSEALSSMVENEATRPLTFSDQYYQTR"
     gene            1175..2179
                     /locus_tag="ACHY3M_004910"
                     /pseudo
     CDS             1175..2179
                     /locus_tag="ACHY3M_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
     gene            complement(2238..3404)
                     /gene="ugd"
                     /locus_tag="ACHY3M_004911"
     CDS             complement(2238..3404)
                     /gene="ugd"
                     /locus_tag="ACHY3M_004911"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFS4319650.1"
                     /translation="MKITISGTGYVGLSNGILIAQHHEVVALDIVPTKVEMLNQKKSP
                     IVDKEIEEYLATKQLNFRATTDKYDAYRDADYVIIATPTDYDPKTNYFNTSSVESVIR
                     DVVDINPNAVMVIKSTIPVGFTNLLKERLGIDNIFFSPEFLREGRALYDNLHPSRIVI
                     GERSERAGRFAALLQEGAVKKDIPTLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYASVPNNIIGAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVII
                     YEPVMVEDEFFHSRVVRDLTAFKNEADIIISNRMTSELADVADKVYTRGLFGSD"
     gene            complement(3603..5009)
                     /gene="gndA"
                     /locus_tag="ACHY3M_004912"
     CDS             complement(3603..5009)
                     /gene="gndA"
                     /locus_tag="ACHY3M_004912"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4319651.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCITYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELATT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRIAASKVLSGPQAKLAGDKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENKGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
BASE COUNT         1392 a         1260 c         1147 g         1355 t
ORIGIN      
        1 tgacgcgttt ggtagtactg gtcagaaaac gtcaaaggac gtgtagcttc gttctcaacc
       61 attgaggaaa gagcctcact acccagcata aacatagaat cctgagatac ttgatcaacc
      121 tgctgtacct gcggtagctt aatattagac tgttgcgcaa ccttaagtgc ttgagtcatc
      181 tgcgcaatac gcagggtttt ctgctctttc gccactgcct cctgcgctgc aagagatttt
      241 tgcaaatcag ccagtcttgt ttccatactc attgacaagt cagcattgag ctctttagca
      301 acaccttcat caacttgttg aatatactga gccagcgttt tttgtgcctc ttcagaggtt
      361 ttgccggtat aagaaacctt taacggcaga gtctgacctt taacagcagc atcaattgaa
      421 agtttttccg gttcttgctg gttatccaac gtttcagcca gtgcagaaaa tgccgaacta
      481 aaacgcccga taaaacccgc ctgaacatct gtcatttcgg gtgcgttagc cccaaacagt
      541 acatccatag cattattgta gctggcaagc tggccagcat cgggttgtgt aacgatagct
      601 atcgaggtcc atttctcctt agccaccgcc aaatagccta ctgccaacgc tattgccaca
      661 acaatgcaga taataatcgt taccttccca cgccataact gcatgagtaa atcaatcaaa
      721 tcaatctgtt ccggatcgtg actgcgaccg gacatgttgt tatcacttat agacatagaa
      781 accctagata aggaaagact taaaggggga tcagtttagc tataaattga ggcaatgcca
      841 taggattaat gataaaaaaa tcaaattgtt tggggaattt tgattggtat ttcaatcact
      901 actggggaac gatacgatta aacaaaagtg tcaatatcta aagaacactt tcatgcgcaa
      961 aaaaccatca acaaacaggt ttatgcccgc caatcgttac actttacaat tcattttatt
     1021 gaattcatat cgtgaatact taccgtctga ccagtaaaaa tggtaagcgt aaaaaaatat
     1081 aacaattagc ttttttacca tctaacggta tcattccact tttaatagca cctgttggtg
     1141 cctggctact atttggccga aaagagggat aaacatgaaa tacctggtta cgggtgccgc
     1201 tggtttcatc ggcttccacg ttagcaaacg cctccttgag gctggccatc aagtagtcgg
     1261 tattgataat ctgaacgatt actacgacgt cagccttaaa caagctcgtc tggaattact
     1321 ggcacaacct ggtttccagt ttcataaaat tgatttagcc gaccgcgaag gcatgaccga
     1381 tctcttcgca tccgggcatt tcgaacgtgt gattcatctc gccgcatagg ctggcgtacg
     1441 ctattcactc gaaaacccgc atgcctatgc tgactcaaat ctcacaggat ttctgaacat
     1501 actggaaggg tgtcgccata ataaaattca gcatttactc tatgcttcct ccagttctgt
     1561 ttacggcctg aatcgcaaaa tgcctttctc tacggatgat tccgttgatc accccgtatc
     1621 tctgtatgcg gcaaccaaaa aagccaacga actgatggcc catacttatt cccatttgta
     1681 cggattgccc gcaaccgggt tgcgtttctt taccgtgtac ggcccctggg gtcgtccgga
     1741 tatggcactg tttaagttca ccaaagcaat gctggaaggg aaaagtattg acgtctataa
     1801 ctacggcaaa atgaagcgtg actttaccta tatcgatgat atcgcagaag caatcattcg
     1861 cctgcaggat gtcattccac acgcagacac acaatggacc gttgaaacag gcacgccagc
     1921 agccagcatt gccccctatc gcgtctacaa tatcggcaat agttcaccgg ttgagctcat
     1981 ggactatatc caggctttgg aagatgcgct gggtattgaa gcaaagaaaa acatgctacc
     2041 gctacagcct ggtgatgtgc tggaaaccag cgcggacact aaagcactgt atgaggtaat
     2101 tggttttacg cctgaaacca cggtcaaaga tggggtgaag aactttgtta actggtaccg
     2161 tgatttctac aaagtttgat tgctgatctg attaaaataa aaaggccttt tataaggcct
     2221 tttttatgga aacgagatta atcactacca aacaaaccac gggtatacac tttatcggca
     2281 acatcagcta attctgacgt catgcggtta gaaataataa tatctgcttc atttttgaac
     2341 gcagtaagat cacgcactac ccgagaatgg aaaaactcat cctctaccat gacgggttca
     2401 taaataataa caggtacccc cttggcttta atacgcttca taatgccctg aatagatgat
     2461 gcccggaaat tatcggaacc acttttcatg atcaaacggt acacgccgac caccttaggc
     2521 ttacgcgcaa gaatagaatc tgcaatgaag tccttacgcg tacggttcgc atcaacgatc
     2581 gcgccaataa tgttattcgg tacagacgcg tagttagcca gcaactgctt agtatctttc
     2641 ggcagacagt agccaccata accgaatgac gggttgttgt agtgattacc gatacgcgga
     2701 tccaggcaca caccttcaat aatctgccgt gagttcaagc caagactttc tgcatagcta
     2761 tccagctcgt taaagtaggc cacacgtaac gccagatagg tgttagcgaa cagcttgatg
     2821 gcttcggctt cagtcgagtc agtaaacagc gtcggaatgt cttttttaac cgcaccttcc
     2881 tgcagcaacg cggcaaaacg cccggctcgt tcggaacgtt caccaataac gatacgggac
     2941 ggatgcaagt tgtcgtacag tgcacgacct tcacgcagga attctggcga aaagaatata
     3001 ttgtcgatac ctaaccgttc tttaagtaag tttgtaaagc caaccggaat tgttgattta
     3061 attaccatca ccgcatttgg gttgatgtcg accacatcgc ggatcactga ctcgacgctc
     3121 gaggtgttaa agtagttagt tttcggatcg taatcagtcg gtgtcgcgat gataacgtag
     3181 tccgcgtcgc ggtaagcatc atacttatcg gtcgttgcac ggaaattcag ctgttttgtc
     3241 gccagatatt cttcgatttc cttatcaacg atcggcgatt ttttctgatt cagcatctcg
     3301 accttcgttg gaacgatatc cagagcgacc acttcgtggt gctgggcaat cagaataccg
     3361 ttcgacaaac caacataacc tgttcctgag atagttatct tcattcgctg tgtaacttct
     3421 tccggttgaa catttgaggg tgaacacgct gccaacaacg tggcacatcg gctctgcatt
     3481 gcatcagtat atcgtgattt gctacatcac cggatatcgg acctgactga atcccatttt
     3541 ttggcaataa aaaagcccgg aggagcgctc cgggcctgga tttacagtta gactaaatca
     3601 gattaatcca gccattcggt gtggaacaca ccttctttat cagtgcgttt atacgtgtgc
     3661 gcaccgaagt aatcacgctg tgcctgaatc agattagccg gcagtaccgc agaacggtag
     3721 ctgtcgtagt aggctaccgc tgcagagaag gtcggtaccg gaataccgtt ctgcacagca
     3781 taagccacta catcacgcag cgcttgctga tattcatcag cgatattttt gaagtacgga
     3841 gccagcaaca ggttagcaat gcctttgttt tcagcatacg cgtcagtaat tttctgcagg
     3901 aactgcgcac gaatgatgca gcccgcgcgg aagatcttcg cgatttcgcc gtagttcaga
     3961 tcccagttgt attcgtcaga cgcggcacgc agttgagaga agccttgggc ataagagacg
     4021 attttaccca ggtacagcgc gcgacggact ttctcaacga actctgcttt atcaccagcc
     4081 agtttagcct gcggaccaga cagcacttta gatgccgcaa tgcgctggtc tttcagagaa
     4141 gagatgtagc gagcgaatac ggattcggtg atcagcgaca gcggttcacc cagatccaga
     4201 gagctctggc tggtccattt accggtgcct ttgttcgcag cttcgtccag gatcacatca
     4261 accaggtatt taccctcttc atcttttttg gtgaagatgt ctttggtgat gtcaatcagg
     4321 tagctactta gctcgccttc attccactcg gtaaaagtgg ttgccagctc ttcgttagac
     4381 agattaaggc cgcctttaag cagagaatag gcttcagcaa tcagctgcat atcgccatat
     4441 tcgataccgt tgtgcaccat cttcacgtag tgacccgcac cgtcagcacc gatgtaagtt
     4501 atacatggtt cgccatcttc agcaaccgca gcaatcttgg tcaggatagg cgcaaccagc
     4561 tcatacgctt ctttctggcc acctggcatg atagatgggc ctttcagggc gccctcttca
     4621 ccgccggaca cgccggtacc gatgaagtta aagccttccg cggacagttc acggttacga
     4681 cggatagtgt cctggaagaa ggtgttgcca ccatcaataa tgatgtcgcc tttatccaga
     4741 tacggcttca gggaatcgat agcagcatca gttcccgccc ctgcttttac cattaacagg
     4801 atacgacgtg gggtttcaag agactcgacg aactctttca ccgtgtaata aggaaccagt
     4861 ttcttatccg ggttctcggc aacaacttct tcagttttct cgcgggagcg gttgaagatg
     4921 gagacggtat aaccgcggct ttcgatgttg agcgccaggt tgcgccccat cactgccata
     4981 ccgacgacgc cgatctgttg cttggacatt acatactcct gtcaggtgta gtcaccgcgc
     5041 gacgcatgcg cggcttgaat gtagtctaga tgaagttgag acagattaat actgatatat
     5101 aacaaaatcc agctataagt aaaaatacgg agccagtaaa aaaacagcat agag
//
LOCUS       DAZUFB010000202         5153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZUFB010000202 DAZUFB010000000
VERSION     DAZUFB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.202"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..1243
                     /gene="proB"
                     /locus_tag="ACHY3M_004913"
     CDS             140..1243
                     /gene="proB"
                     /locus_tag="ACHY3M_004913"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4319652.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1255..2508
                     /gene="proA"
                     /locus_tag="ACHY3M_004914"
     CDS             1255..2508
                     /gene="proA"
                     /locus_tag="ACHY3M_004914"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4319653.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCRDQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2623..2698
                     /locus_tag="ACHY3M_004915"
     tRNA            2623..2698
                     /locus_tag="ACHY3M_004915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2656..2658,aa:Thr,seq:cgt)
     gene            2864..4078
                     /locus_tag="ACHY3M_004916"
     CDS             2864..4078
                     /locus_tag="ACHY3M_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4319654.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSDQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDALEKE
                     ENVVHGSFGKRA"
     gene            4221..5102
                     /locus_tag="ACHY3M_004917"
     CDS             4221..5102
                     /locus_tag="ACHY3M_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFS4319655.1"
                     /translation="MVRAKKQDLAWFDISNYEFLNDLTLPDLIQELEWRDFLLRHAKD
                     DTAIFKEEYDIKYERIFSGDPNLTILNEEEEEVEEFIRKVNDEAPSLRNEYDDLPSLS
                     SAMGVSPVTFSELAMYSFSSIDQGFFKRDEEDCYLKANAMLASVTGNLSNCSPNIILV
                     SIDLDDATDDEIITSLTHLLPLWRKELKVPEREHVAQKRIGLKTLQKLISNRVIPIVD
                     LLIWGEKSGKEVSNPMISALVFSDDPKDTQAIKESIKPFALESISERYTRLLQLYVNK
                     DREMSLIKISDLMSRDL"
BASE COUNT         1370 a         1180 c         1361 g         1242 t
ORIGIN      
        1 cgtaaacaac tgccgctagg cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc
       61 agatggttgg caaccgacga cagtcctgct aaaacgttcg tttgatatca tttttcctaa
      121 aattgaatgg cagagaatca tgagtgacag ccagacgctg gtggtaaaac tcggcaccag
      181 tgtgctaaca ggcggatcgc gccgtctgaa ccgtgcccat atcgttgaac ttgttcgcca
      241 gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc
      301 cgccggacgt gagcacctgg gttacccgga actgccagcg actatcgcct cgaaacaact
      361 gctggcagcg gtagggcaga gtcgactgat tcaactgtgg gaacagctgt tttcgattta
      421 tggcattcac gtcgggcaaa tgctgctgac ccgtgctgat atggaagacc gtgaacgctt
      481 cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa
      541 tgagaacgat gctgtcgcta cggcagagat taaagtcggc gataacgata acctttctgc
      601 gctggcggcg attctggcgg gtgccgataa actgttgctg ctgaccgatc aaaaaggttt
      661 gtataccgct gacccgcgca gcaatccgca ggcagaactg attaaagatg tttacggcat
      721 tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca ggcctcggaa ctggcggcat
      781 gagtaccaaa ttgcaggctg ctgatgtggc ttgccgtgcg ggtatcgaca ccattattgc
      841 cgcgggcagc aagccgggcg ttattggtga tgtgatggaa ggcatttccg tcggtacgct
      901 gttccatgcc caggcgactc cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc
      961 ggcgggtgaa atcacggtag atgaaggggc aactgccgcc attcttgaac gcggcagctc
     1021 cctgttgccg aaaggcatta aaagcgtgac tggcaatttc tcgcgtggtg aagtcatccg
     1081 catttgcaac ctcgaaggtc gcgatatcgc ccacggcgtc agtcgttaca acagcgatgc
     1141 attacgccgt attgccgggc accactcgca agaaattgat gcaatactgg gatatgaata
     1201 cggcccggtt gccgttcacc gtgatgacat gattacccgt taaggagcag gctgatgctg
     1261 gaacaaatgg gcattgccgc gaagcaagcc tcgtataaat tagcgcaact ctccagccgc
     1321 gaaaaaaatc gcgtgctgga aaaaatcgcc gatgaactgg aagcacaaag cgaaatcatc
     1381 ctcaacgcta acgcccagga tgttgctgac gcgcgtgcca atggccttgg cgaagcgatg
     1441 cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca ttgccgatga tgtgcgccag
     1501 gtgtgtaacc tcgccgatcc ggtggggcag gtaatcgatg gcagcgtact ggacagcggc
     1561 ctgcgtcttg agcgtcgtcg cgtaccgctg ggggttattg gcgtgattta tgaagcgcgc
     1621 ccgaacgtga cggttgatgt cgcttcgctg tgcctgaaaa ccggtaatgc ggtgatcctg
     1681 cgcggtggca aagaaacgtg tcgcactaac gctgcaacgg tggcggtgat tcaggacgcc
     1741 ctgaaatcct gcggcttacc ggcgggtgcc gtgcaggcga ttgataatcc tgaccgtgcg
     1801 ctggtcagtg aaatgctgcg tatggataaa tacatcgaca tgctgatccc gcgtggtggc
     1861 gctggtttgc ataaactgtg ccgtgaccag tcgacaatcc cggtgatcac aggtggtata
     1921 ggcgtatgcc atatttacgt tgatgaaagt gtagagatcg ctgaagcatt aaaagtgatc
     1981 gtcaacgcga aaactcagcg tccgagcaca tgtaatacgg ttgaaacgtt gctggtgaat
     2041 aaaaacatcg ccgatagctt cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg
     2101 acattacacg cagatgcagc tgcactggcg cagttgcagg caggccctgc gaaggtggtt
     2161 gctgttaaag ccgaagagta tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc
     2221 gtcagcgatc ttgacgatgc catcgcccat attcgtgaac acggcacaca acactccgat
     2281 gcgatcctga cccgcgatat gcgcaacgcc cagcgttttg ttaacgaagt ggattcgtcc
     2341 gctgtttacg ttaacgcctc tacgcgtttt accgacggcg gccagtttgg tctgggtgcg
     2401 gaagtggcgg taagcacaca aaaactccac gcgcgtggcc caatggggct ggaagcactg
     2461 accacttaca agtggatcgg cattggtgat tacaccattc gtgcgtaaat aaaaccgggt
     2521 gatgcaaaag tagccatttg attcacaagg ccattgacgc atcgcccggt tagttttaac
     2581 cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca gggccgatat agctcagttg
     2641 gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt
     2701 caaacatctc cccaagtcta ctaaagtctt tcaaaacccc ttataatccg tgtattaaag
     2761 ccccgttcgt cttttgacgt ctactaaagt ccctcaaaat ctacggtcag atgggggtac
     2821 ttatgggggt atttgctgtt cggtttagtg gaggtacccc caagtgaaac tcaatgcccg
     2881 tcaaatagac acagccaagc caaaagagaa ggcttacaag ctggccgatg gtggcggttt
     2941 gtatctcttg gtaaaaccta atggaggtaa atactggcga cttaaatatc gtgtagctgg
     3001 taaagagaag ctattggcac taggtgtgta tcctgaggtt actctagccg atgctcgggc
     3061 aaaacgtgaa gatgcgaaaa gaggtatcgc tggtggtatc gatccgatgg aagcgaaacg
     3121 agaggaaaag attgcccggg aaacgcagtt aaacaacacc ttcaaagata ttgcccttga
     3181 gtggcacagc agcaaattaa aaaaatggtc tgctggttat gcttcagaca tcctcgaagc
     3241 cttcaacaaa gatgtgttcc cttacattgg caaaaaacca atcgccgaaa tcaaaccact
     3301 tgaacttctg aatgtgctgc ggcgcatcga ggggcgcggt gctacagaaa aagccaaaaa
     3361 agtgaggcag cggtgcgggg aagttttccg ctatgcaata gtcactggtc gcgcagagta
     3421 taaccctgca ccagacctta ctagcgcgat gcaaggccat gaatctaatc attacccttt
     3481 ccttacagct aaagaattac ctgatttttt caaggcattg tccagttact caggaagcgc
     3541 attggttgtt atggcggctc gtctactgat tatcaccggc ttgcggactg gcgaactacg
     3601 tggcgcatta tgggatgaaa ttgatttcaa caaggctatc tgggagatac ccgcttcacg
     3661 tatgaaaatg cggcggcctc atattgtgcc attgtctgat caggctcttt cgcttattgg
     3721 gaagattaga gaaataacag gcaattaccc tcttatgttt cccgggcgca atgatccaag
     3781 gaaaacaatg agtgaggcca gcataaacca agtgtttaag cgcattggct acgctggacg
     3841 tgtaactggt catgggttcc ggcacactat gagcacgatt ttgcatgagc agggctataa
     3901 caccgcgtgg atagaaacgc agctcgctca cgttgataag aactcaattc gtggcacata
     3961 caaccatgcg caatatctgg atggaaggcg ggagatgctt caatggtatg ccgactatat
     4021 ggatgcgctg gagaaggagg agaacgttgt tcatggttcg tttgggaaac gagcttaact
     4081 gtatgaatag accgtgttaa gcaatggtag tagacttatg tagacgaaca aagaataggc
     4141 tatgtctagg ctgatccccg aaaacccgta cacccctacg ggctggcata gccgtcagaa
     4201 ttaggggcgc ggggtggcgt atggtgagag ccaaaaaaca agatttagca tggtttgata
     4261 ttagtaatta tgaattttta aatgatttaa ccttgccgga cttgattcaa gaattagagt
     4321 ggcgcgattt tttattgcgt catgcaaaag atgacactgc tatattcaaa gaagagtacg
     4381 atataaaata tgaaaggatt ttttcgggcg atcctaacct aaccattctc aatgaagaag
     4441 aggaagaggt cgaagaattt attcgcaaag taaatgatga agccccatca ttaagaaatg
     4501 agtacgatga tcttccctca ttatcttcag ctatgggggt tagtccagta acattttcag
     4561 agctggccat gtactctttc tcatcgatag atcaaggctt ttttaaaaga gacgaagagg
     4621 attgctacct caaggcaaac gctatgcttg cgagtgttac tggaaaccta agtaattgtt
     4681 cccctaacat catactcgtt tctatagatt tagatgacgc cactgatgac gaaattatta
     4741 ccagcctgac tcaccttcta cctctatggc gaaaagagtt aaaagtacca gagagagagc
     4801 atgtcgctca gaaaaggatt ggattaaaga cccttcagaa gttgatatct aatagagtga
     4861 tacctattgt tgatctgctg atttggggtg agaagtcagg taaggaagtg agcaatccga
     4921 tgatttctgc cttggttttt agtgatgatc cgaaagatac gcaggctatt aaggaatcaa
     4981 taaagccttt tgcacttgaa tctataagcg agagatacac ccgcttgctt cagctttatg
     5041 taaacaaaga cagagaaatg agcttgataa aaatatctga tttaatgagt agagatttat
     5101 agcaagcaaa atcctacgta aacgttttaa gtaaatttcg cgatttccgc ttt
//
LOCUS       DAZUFB010000203         5110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZUFB010000203 DAZUFB010000000
VERSION     DAZUFB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.203"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..878
                     /locus_tag="ACHY3M_004918"
                     /pseudo
     CDS             1..878
                     /locus_tag="ACHY3M_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
     gene            876..1665
                     /locus_tag="ACHY3M_004919"
                     /pseudo
     CDS             876..1665
                     /locus_tag="ACHY3M_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1693..1782)
                     /locus_tag="ACHY3M_004920"
     tRNA            complement(1693..1782)
                     /locus_tag="ACHY3M_004920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1746..1748),aa:Ser,seq:cga)
     gene            1876..2673
                     /gene="mtfA"
                     /locus_tag="ACHY3M_004921"
     CDS             1876..2673
                     /gene="mtfA"
                     /locus_tag="ACHY3M_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFS4319656.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2774..2849
                     /locus_tag="ACHY3M_004922"
     tRNA            2774..2849
                     /locus_tag="ACHY3M_004922"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2807..2809,aa:Asn,seq:gtt)
     gene            3029..4303
                     /locus_tag="ACHY3M_004923"
     CDS             3029..4303
                     /locus_tag="ACHY3M_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4319657.1"
                     /translation="MSLTDTKVKNARPAEKAVKLTDGFGLYLLVHPNGSKYWQLGYRF
                     DGKQKVFSIGVYPAVSLADARQRRDEAKKLLAQGVDPNAKKLADEKALQEKRDKTCSF
                     RVVARSWFATKTKWSEDYADTVWKRLETYVFPDIGDSNVSDLDTGDLLVPIKNAETLG
                     YLEIAMRIKQYITAILRHAVQQKLMRHNPAYDMEGAVQKPETEHRPALELEEIPLLLE
                     RIDAYKGRGLTTLAIKLNLLIFIRSSELRYARWSEIDFNSKLWVIPEKREAIERVKYS
                     TRGAKMKRQHFVPLCRQALKILKEIRQLTYEEGNEAGLIFTGCYDSFKPMSENTINKA
                     LRKMGYDTTQDICGHGFRTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKH
                     LEQRRLMLQWWADYLDANRDGMVRPFEFAQKQ"
     gene            complement(4373..4624)
                     /locus_tag="ACHY3M_004924"
     CDS             complement(4373..4624)
                     /locus_tag="ACHY3M_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319658.1"
                     /translation="MSKNVKQTSENVASTAAKTLTNPNASAIQKSLAGSALSQRGTSN
                     QTSGQMEHKASSALDNPRSSKLTKQLAASVLAQSNKGRK"
BASE COUNT         1491 a         1130 c         1201 g         1288 t
ORIGIN      
        1 tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga aacattgacg
       61 gcagcatcca tcgctgggca aaaaatgaag ttatcgacaa cggccgcgaa gactggaagc
      121 gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc cagactattt
      181 ttggccttgt ccgtgaacgt ccgatcgaca ttcacaaaga ccctgtggca ctgaacaaat
      241 acattactga atacctgact acaaagggcg tgtttgaaga tgaaggaaca aatcagagcg
      301 caactgacac tctctcgtcg ccagtaccag aaactgatgc agtggaaacg gcaattccgg
      361 acaacgaaaa aaccgaatgc aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt
      421 tctacttcct cttcaccgac aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg
      481 gtctggataa ggcgctgtct gctggggcta ctgaaatcac gaaagaagaa tatttcgccc
      541 gcaaaaacgg tacatactca ggttcacaac aaaatactgg tgcatctgac acgaccgcac
      601 aaccagggtc agtaaaagtt accgctgacg aagtaaacaa aattatgcag gcagccaata
      661 tcagccagcc tgacgccgat gaactgcttg cagtatcacg tggtgaattt gttgaaggga
      721 ttagcgaccc gaatgatccg aaatgggtta aggggatcca gacccgcgat tctgtgaacc
      781 agaaccagca agaaacggaa cagaacgacc agaaagcgga acaaaacagc ccaaatacgc
      841 aacaaaacga gccagaaacg aaacaacctg aaccactaca acatcaaaaa aagttgcttg
      901 ccgtattcgg taaggtgccg gcagattcca taaaaccaga acacatccgt cgatacatgg
      961 acaagagagg ggagcagagt aaaacgcaag ccaaccatga aaaaagcagt atgtcccgcg
     1021 tttacagttg ggggtatgag cgagggtacg tgaagggtaa cccatgtgca ggtgtaagta
     1081 aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag gcagtattaa
     1141 gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt gcagcgaggg
     1201 tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacggaatc tttatccagc
     1261 aagggaaaac aggaaaaaaa cagataaaag catggagtcc acgattacag gcggcgatcg
     1321 aaaaagcaaa acagttacca acatccgcct atgtaatcag taatcaatac ggcaaccgat
     1381 atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcgcgca ggcaaaattt
     1441 caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt tcagactatg
     1501 aaggaagcag tcgggataag caacttttct ctggtcacaa aaccgagggg caagtgctaa
     1561 tctatgacag gaaggttaaa gtttcaccga cacttgatgt cccgttacct gaaaatattc
     1621 caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa tgatgtataa
     1681 gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg cccccactcc
     1741 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     1801 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     1861 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     1921 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac ggaacaggaa
     1981 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     2041 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     2101 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     2161 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     2221 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     2281 gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca taagctggac
     2341 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     2401 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     2461 gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg ttttgccgta
     2521 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     2581 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatcg cactaatgat
     2641 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     2701 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgcgcc
     2761 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     2821 cactggttcg agtccagtca gaggagccaa tttaagggaa gcagacgttc actgacgtct
     2881 gctttctgca tttatatcaa ctggttactc ccttcttcag gttcaccctc gttcgttaaa
     2941 aatcgctcga acccacacta ctttgctggt aaaaatgctg gtaaagctgg ttcgatttgc
     3001 gttttaccag cacgcggagg gaaccgtcat gtcacttact gatactaaag taaaaaatgc
     3061 cagaccagcg gaaaaagccg tcaagctcac tgatggattt ggtctctacc ttcttgtgca
     3121 tcccaatggt tcaaaatact ggcagttagg ctatcgcttc gatggtaaac agaaagtgtt
     3181 ttccattggt gtttaccctg cggtttctct tgctgatgcc agacaacgac gtgatgaagc
     3241 aaaaaagctg cttgctcagg gagtcgatcc taacgctaaa aaactggctg atgaaaaagc
     3301 gctgcaggaa aagcgggata aaacctgttc gttccgtgtc gtcgccagaa gctggtttgc
     3361 caccaaaaca aaatggtcag aagattacgc cgatacggta tggaagcgcc ttgagaccta
     3421 tgtattcccg gatattggcg acagcaacgt ttcagatctg gatacgggtg atctgcttgt
     3481 tccgattaaa aatgcagaaa cactcggcta ccttgaaatt gccatgcgga ttaagcaata
     3541 catcaccgcg atcctacgtc atgccgtcca gcaaaagctt atgcgtcata atccggccta
     3601 tgatatggaa ggcgctgtcc agaaaccaga gacggaacac cgccctgcac tggagctgga
     3661 agagatcccg ctactgcttg aacgtattga tgcctacaaa ggtcgtggac taactacact
     3721 ggcgattaaa ctcaatctgt tgattttcat tcgttccagt gaactccgct atgcacgatg
     3781 gtcagaaatc gacttcaaca gtaagttatg ggtaatacca gaaaagcgtg aagcgattga
     3841 gcgcgtcaaa tattccacgc gtggcgcaaa aatgaaacgc cagcacttcg ttcccctctg
     3901 caggcaggct cttaaaatac tgaaagagat ccgccagctt acctatgaag aaggtaatga
     3961 ggcggggtta atttttacgg gctgttatga ctcgttcaag cccatgagtg aaaacaccat
     4021 caataaagcg ctacgtaaga tgggctatga caccacgcag gacatctgcg gtcatggttt
     4081 ccgcacgctg gcatgtagtg ctctgattga atcagggcta tggtctgaag atgcggtaga
     4141 acttcaaatg agccacaagg aaagcaacag cgttcgtgct gcctataccc ataaggccaa
     4201 acatcttgag cagcgaaggt tgatgctaca gtggtgggca gattatcttg atgcaaaccg
     4261 ggatgggatg gtcaggccgt ttgagtttgc tcagaaacaa taatcgcctt ccagcaagca
     4321 gacaatcgta gtaaataaaa aacgcaggga ggtttactct ccctgtaatt tgtcacttac
     4381 gccctttgtt tgattgtgcc aaaacagagg ctgcaagttg cttcgttaac ttgctggaac
     4441 gtggattatc aagagctgat gatgccttat gctccatttg cccacttgtc tggtttgatg
     4501 tcccacgttg tgaaagcgcg gaaccagcga ggcttttctg gatagctgaa gcgttcggat
     4561 tagtcagcgt ttttgcagcg gtagaagcca cattttcaga agtctgtttt acgtttttac
     4621 tcatgaaacg aatccccaag aagttatgaa tattccttat gcagtacttt catttctaaa
     4681 aaaacactat atgaagtgat aaccatattt tttataagcc ttaattttgc taaaggcaat
     4741 attattgctg tataaaatta cagtaaaagt ttaattgatt gattttaatc ataaaaatat
     4801 tcattcagac aggaacaaga gacgccagaa gctccttatc ttccttaaat ggttgccttc
     4861 tgaaaatcaa gattgagggc cgtgttaaca ttacgcgggt aagatcattg aacatacata
     4921 gctcagcagt tagcagataa ggctgaagca acgtatcaac gagggaacgc ggttgcgctg
     4981 aaggatgatt ggacggcgta ttcttttacc aatatttaac tgctctgttt gaaaacaccg
     5041 ttatgttccc tatctctcat aaaggacaac ttcctgacac cagcttttct ggttgtatta
     5101 aataaatgat
//
LOCUS       DAZUFB010000204         5085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZUFB010000204 DAZUFB010000000
VERSION     DAZUFB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.204"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..895
                     /gene="yneK"
                     /locus_tag="ACHY3M_004925"
                     /pseudo
     CDS             1..895
                     /gene="yneK"
                     /locus_tag="ACHY3M_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1045..2235
                     /gene="ydeA"
                     /locus_tag="ACHY3M_004926"
     CDS             1045..2235
                     /gene="ydeA"
                     /locus_tag="ACHY3M_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFS4319659.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2260..2925)
                     /gene="marC"
                     /locus_tag="ACHY3M_004927"
     CDS             complement(2260..2925)
                     /gene="marC"
                     /locus_tag="ACHY3M_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFS4319660.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3137..3571
                     /gene="marR"
                     /locus_tag="ACHY3M_004928"
     CDS             3137..3571
                     /gene="marR"
                     /locus_tag="ACHY3M_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFS4319661.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3591..3974
                     /gene="marA"
                     /locus_tag="ACHY3M_004929"
     CDS             3591..3974
                     /gene="marA"
                     /locus_tag="ACHY3M_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFS4319662.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4006..4224
                     /gene="marB"
                     /locus_tag="ACHY3M_004930"
     CDS             4006..4224
                     /gene="marB"
                     /locus_tag="ACHY3M_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFS4319663.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4281..>5085)
                     /locus_tag="ACHY3M_004931"
     CDS             complement(4281..>5085)
                     /locus_tag="ACHY3M_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFS4319664.1"
                     /translation="AVHYELVASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDM
                     MMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFS
                     YYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLP
                     LFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDL
                     VSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1333 a         1238 c         1150 g         1364 t
ORIGIN      
        1 ttcggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact tatcccgtta
       61 agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta agccctattt
      121 atttagataa ttttcgccat gatggagaaa ttaatatttt tgcgctccca acccaaagcc
      181 tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctatttc tgtttctgaa
      241 tgattttatt gaacaatttc caatgatcaa ccttggcgtt cccatcaaaa gagcgcatac
      301 accacatatt gaacccctgc ctccggatcg ccataccgct gcagattact tacgccagtt
      361 tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc cccgattatg
      421 gagtaactct gaggttcaca gatggttcgt caataaagat cctaacttga tcaccgccat
      481 tctcgacata acagacggcg aattaaaaga ggatttattg caaagcctga tggattcatt
      541 aggttctaac aaacatgtac tacccgaagt ctgcatctgc tttttatccc ttttagcaga
      601 acaagaatct cctcattttc aggacttttt tctatttttc gccaatatgt tattgcacta
      661 tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc cagcatcatt
      721 aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactgt ttgtaaaaaa
      781 tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt
      841 cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc attaaaccgt
      901 gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga aaacgcgatc
      961 cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt catcactttt
     1021 tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa agtggcgtgg
     1081 ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga atttgtccct
     1141 gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatt
     1201 atgttgacca tttatgcatg ggtagtggcg ctaatgtcat tgccttttat gttaatgacc
     1261 agccaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat tgccagccac
     1321 gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat tggtgtggct
     1381 tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat ggctccggcc
     1441 gggaagcgag cacaggcatt gagtttaatt gccaccggta cagcactggc gatggtctta
     1501 ggtttacctc tcgggcgcat tgtgggccag tatttcggtt ggcgaatgac cttcttcgcg
     1561 attggtattg gggcgcttat cacccttttg tgcctgatta agttacttcc cttactgccc
     1621 agtgagcatt ccggttcact gaaaagcctc ccgctattgt tccgccgccc ggcattgatg
     1681 agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata cagctatatc
     1741 gagccttttg tacaaaacat tgcgggattc agcgccaact ttgccacggc attactgtta
     1801 ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg taatcagtat
     1861 gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg tgtgcctggc attgctgtta
     1921 cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg gatcgcgatg
     1981 atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac
     2041 gtcgcgatgg cgctattctc cgggatattt aatattggaa tcggggcggg tgcgttggta
     2101 ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct
     2161 gcttttgccg cgttaatttg gtcaatcatt atatttcgcc gctggccagt gacactcgaa
     2221 gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg ttttaatgat
     2281 ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga atcccatcag
     2341 acgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc cggagctgcg
     2401 taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg caaccatcag
     2461 tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa taatcatcgc
     2521 tatggttccc ggaccagcgg tacttggcat tgctagcggc acaaaggcaa tattggcact
     2581 gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg
     2641 ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt aagccgcctg caattcgcag
     2701 accgggaatc gaaatgccaa atgtatccat caccagttgc ccggcgtaat acgccaccat
     2761 catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt cggcactgtt
     2821 catgttgcct gccaggccaa gaaataacgc gacagttgtt aatgggttag ctaacggcag
     2881 taacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg gttgttatcc
     2941 tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag tgtcactctt
     3001 tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg
     3061 tcaattcatt tatttgactt atacttgcct gggcaatatt atcccctgca actaattact
     3121 tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta ttccattggg
     3181 tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt atctgtctcc
     3241 gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg cggcgtgtat
     3301 tactccggtt gaactgaaaa aagtgttgtc tgtcgacctt ggagcactga cccgtatgct
     3361 ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg ataagcgcgg
     3421 cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt
     3481 tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag tggcaacact
     3541 tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg atgtccagac
     3601 gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat
     3661 cgccactgtc actggagaaa gtgtcagagc gttcaggtta ctctaaatgg cacctgcaac
     3721 ggatgtttaa aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga
     3781 cggaaatcgc gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat
     3841 atggcttcga gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc
     3901 cgcataaata ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc
     3961 attacaacag ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttca
     4021 tccgcaatag cagctgcgct tattctcttt tccgcgcagg gcgttgcgga acaaaccacg
     4081 cagccagttg ttacttcttg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca
     4141 ccgtttgatt taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc
     4201 tattataatc aacacgctat gtagttattg tcaggctctg atcacagagc ctgacatctc
     4261 cgtaatatct tcctcaagtc tcaaatattt tcgccattgt tggcgatgag atcccgatac
     4321 cagtaaaacg acgctttacg tatccgttcc agcgttccct tcccttcgtt gtctttatcg
     4381 acataaatca ttccgtagcg ctttttcatt tcccctgttc cggcagaaac caggtcaatg
     4441 cagccccacg gggtgtagcc aattaagtca acaccatctt caactacggc ttttttcatt
     4501 tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct
     4561 tgtctctggt caaccgcacc aaatccattt tcgacaataa acagcggcaa ctggaaatga
     4621 tcccagaacc agttgagtga ataacgtagc cctgccggat caatttgcca tccccattcg
     4681 gatgtatcga tataagggtt gctgaccaaa tctcgtggtt caacataatc cagttgcggg
     4741 ttatcgggcg aaaattgtgt cgtaaaagac atgtagtagc taaagccgat aaagtcgaca
     4801 caaccactgg caagaatcgc gttatcttct ggtgtgatat cgaggttgaa tcctttcctg
     4861 gcaaagtaat tcagcatatg ttgcggataa tatccacgag catgaacatc agtaaaccag
     4921 taacgacgat gcattgcttt cgtggccatc atcatatcgt tgggtgcaca cgtcagagga
     4981 tagatgggac acatggcgat catacagcca atgttaaatt caggattgat cgattttcca
     5041 gtctgtaccg ccagggcact ggcaactaac tcgtaatgta ccgcc
//
LOCUS       DAZUFB010000205         4954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZUFB010000205 DAZUFB010000000
VERSION     DAZUFB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.205"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHY3M_004932"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHY3M_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4319665.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1047..1122)
                     /locus_tag="ACHY3M_004933"
     tRNA            complement(1047..1122)
                     /locus_tag="ACHY3M_004933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1087..1089),aa:Thr,seq:ggt)
     gene            complement(1129..1203)
                     /locus_tag="ACHY3M_004934"
     tRNA            complement(1129..1203)
                     /locus_tag="ACHY3M_004934"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Gly,seq:tcc)
     gene            complement(1320..1404)
                     /locus_tag="ACHY3M_004935"
     tRNA            complement(1320..1404)
                     /locus_tag="ACHY3M_004935"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1368..1370),aa:Tyr,seq:gta)
     gene            complement(1413..1488)
                     /locus_tag="ACHY3M_004936"
     tRNA            complement(1413..1488)
                     /locus_tag="ACHY3M_004936"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Thr,seq:tgt)
     gene            1850..2800
                     /gene="coaA"
                     /locus_tag="ACHY3M_004937"
     CDS             1850..2800
                     /gene="coaA"
                     /locus_tag="ACHY3M_004937"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4319666.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2829..3794)
                     /gene="birA"
                     /locus_tag="ACHY3M_004938"
     CDS             complement(2829..3794)
                     /gene="birA"
                     /locus_tag="ACHY3M_004938"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFS4319667.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3791..4819)
                     /gene="murB"
                     /locus_tag="ACHY3M_004939"
     CDS             complement(3791..4819)
                     /gene="murB"
                     /locus_tag="ACHY3M_004939"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4319668.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1256 a         1261 c         1125 g         1312 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatgtaag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtca tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcgtattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcgcg agcagcaccg ccgtaggttt tagccagtac ggtagtgatt
      841 gcagcggtca gcgttgtttt accatggtca acgtggccga tagtaccgac gttaacgtgc
      901 ggttttgtac gttcaaactt ttctttagac atcgattgtc cctctaagac acggataaat
      961 cggtgatatc accacatcaa ccaggcaaca tgcccgactt gttgaatgca ataaacagaa
     1021 ggaaaaaaca gggaggagaa aagaagtggt gctgataggc agattcgaac tgccgacctc
     1081 acccttacca agggtgcgct ctaccaactg agctatatca gcacatcttg gagcgggcag
     1141 cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc
     1201 cgcatcctgg aactcggcta cctgattttc attctgcact gaatatcgag agaagctctc
     1261 tttattcgag ccggtaagcg aacttatcgt ctcgggctac gccatcgcgt ggccgaaatt
     1321 ggtggtgggg gaaggattcg aaccttcgaa gtctgtgacg gcagatttac agtctgctcc
     1381 ctttggccgc tcgggaaccc caccggactt gatggtgccg actaccggaa tcgaactggt
     1441 gacctactga ttacaagtca gttgctctac ctactgagct aagtcggcat caagtagcgc
     1501 gcattctatg gagacatgcg agttcatgca actaaaaaat tgcataattt gttttattgg
     1561 tcacatttta tgcgacacga tgaagaaaca gccgaacgga gggcagatta gcacactttt
     1621 tcaacatcat tgtgctcaac aatgcgctcc tgctaaacca taattctttt tatcagatgg
     1681 aatatctgtc acattgcttt tcaacgatag cttcctggca gagatttttt cttattattc
     1741 ctccccatct ggtgttaccc tcctgcccat taacccattc aacagaactg tgacgcgcca
     1801 tggcaaatat cgctttgccg atagagctat gaccgccaga aacatgctta tgagtataaa
     1861 agagcaaacg ttaatgacgc cttacctaca gtttgaccgc aaccagtggg cagctctgcg
     1921 tgattccgta cctatgacgt tatcggaaga tgagatcgcc cgtctcaaag gtattaatga
     1981 agatctctcg ttagaagaag ttgccgagat ctatttacct ttgtcacgtt tgctgaactt
     2041 ctatataagc tcgaatctgc gccgtcaggc agttctggaa cagtttcttg gtactaacgg
     2101 gcaacgcatt ccttacatta tcagtattgc tggcagtgtc gcggtgggga aaagcacaac
     2161 cgcccgtgta ttgcaggcgc tattaagccg ttggccggaa catcgtcgtg ttgaactgat
     2221 cactacagat ggcttccttc accctaatca ggttctgaaa gaacgtggtc tgatgaagaa
     2281 gaaaggcttc ccggaatcgt atgatatgca tcgcctggtg aagtttgttt ccgatctcaa
     2341 atccggcgtg ccaaacgtta cagcccctgt ttactcgcat cttatttatg atgtgatccc
     2401 ggatggagat aaaacggttg ttcagcctga tattttaatt cttgaagggt taaatgtctt
     2461 acagagcggg atggattatc cacacgatcc acatcatgta tttgtttctg attttgtcga
     2521 tttttcgata tatgttgatg caccggaaga cttacttcag acgtggtata tcaaccgttt
     2581 tctgaaattc cgcgaagggg cttttaccga cccggattcc tattttcata actacgcgaa
     2641 attaactaaa gaagaagcga ttaagactgc catgacattg tggaaagaga tcaactggct
     2701 gaacttaaag caaaatattc tacctactcg tgagcgcgcc agtttaatcc tgacgaaaag
     2761 tgctaatcat gcggtagaag aggtcagact acgcaaataa tttgcagggg agcgaatact
     2821 cccctttctt atttttctgc actacgcagg gatatttcac cgcccatcca gggttttatt
     2881 attccatcct gctcaagtaa taaagccccc tgtttatcta ttccgcgtga aatgccaaat
     2941 atttctttat caccaatgat aagtttcact gggcgattaa taaaattatc cagcttttcc
     3001 cagcgcgaca gataaggtgc caatccttct tgttcgaaga gttccaacgc agcacgtaat
     3061 tcacgtatta gcatggccgc caacgtatta cgatcgagat tgatccccgc ttcctgcagc
     3121 gtgatccacc cctgattaac gacactctct tcaacacggc gcattgccat gttgatcccg
     3181 gctccaatga ctatttgcgc cgcatcgcca gttttgccag tcagctccac aagaatgcct
     3241 gccagcttgc gatcctgcag atagaggtca ttaggccatt tgacacgaac tttatctgct
     3301 cccagcttgc gtaatacttc cgccatcacg ataccgataa ccagacttaa accaatcgcc
     3361 gccgccgggc cttgttccag acgccagaac atcgacaaat ataagtttgc gccaaaaggc
     3421 gaaaaccatt tccgcccccg gcgaccacgg ccagcctgct ggtattctgc aacacaggca
     3481 tcgcccgatt taagctctcc gatacgatca agaaggtact gattcgtgga gtcaataact
     3541 ggcagcacgg ctacactacc gccatccagc tgacccaata tctgttcagc attaagtaac
     3601 tggatgggct caggcaggct gtatccttta cccggaacgg taaagacatc aacgccccag
     3661 tcacgcagtg tctgaatgtg tttattaata gccgcccggc tcattcccag cgtttcaccc
     3721 aactgctcgc cagagtgaaa ttcaccgttc gctaacaggg caatcaattt cagtggcacg
     3781 gtgttatcct tcatgaaatt gtctccactg cgctcacttc acctgatgca ccaataaagc
     3841 ggacttcagg ctcaagccag acattaaatt tttcacccac tttttgtctt acatggtgtg
     3901 ccagttgcac cacatcttcg ctttttgcat tgtcttcatt aatgagaact aacgcctgtt
     3961 gacggtgcac cgcagcccca cccatttgca tcccttttag ctggcactga tcaataagcc
     4021 aacctgctgc cagttttact gaaccacccg cctgggggta atttggtgct gttggaaatt
     4081 gcgccagtaa tgctttagcc gtttcggcag atacaacagg gtttttgaag aaactaccgg
     4141 cattgccatt cacttttgga tcagggagtt tggtggtgcg catatgacac accgcattaa
     4201 atacttgctg tggcgttact gttgtaggat ccagacgagt taagtcacca tacgttagta
     4261 caggttgcca ctcttttggc agacgcagac ctacggctac aatggcgaag cgatcctggt
     4321 attcatgttt aaaaatactg tcgcgatagc caaaacggca ctctttggca gttaagcgca
     4381 cttgcttgcc tgtcgccagt tcaacacaat caacataagc gcaaactcgc tgtaattcta
     4441 cgccataagc accaatattc tggataggtg atgagccgac acaaccagga attaatgcca
     4501 gattttccag accaggcata ccttcctgca aagtgtattt taccaggcga tgccagtttt
     4561 ctccggctcc tacatgtaaa taccacgcat caggttcatc atgaatttcg atacctttga
     4621 tccggttgat gatcaccgtg ccgcgatagt cttccagaaa aagtacatta cttccttcac
     4681 ccagaataag aacgggttgt ccttctgcgg ttgcatactg ccaggcattg agtaattgtt
     4741 gttcgtcttc ggcacataca atgtgctgag cattatgatc aatgccaaat gtgttccagg
     4801 gttttaagga gtggttcata gctgctttcc tgatgcaaaa acgaggctag tttaccgtat
     4861 ctgtgggggg atggcttgta gatatgacga caggaagagt ttgtagaaac gcaaaaaggc
     4921 catccgtcag gatggccttc tgcttaattt gatg
//
LOCUS       DAZUFB010000206         4821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZUFB010000206 DAZUFB010000000
VERSION     DAZUFB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.206"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2515)
                     /gene="fimD"
                     /locus_tag="ACHY3M_004940"
     CDS             complement(<1..2515)
                     /gene="fimD"
                     /locus_tag="ACHY3M_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFS4319669.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNRSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMP"
     gene            complement(2582..3307)
                     /gene="fimC"
                     /locus_tag="ACHY3M_004941"
     CDS             complement(2582..3307)
                     /gene="fimC"
                     /locus_tag="ACHY3M_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS4319670.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADDVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(3344..3883)
                     /locus_tag="ACHY3M_004942"
     CDS             complement(3344..3883)
                     /locus_tag="ACHY3M_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4319671.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFIIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(3948..4496)
                     /gene="fimA"
                     /locus_tag="ACHY3M_004943"
     CDS             complement(3948..4496)
                     /gene="fimA"
                     /locus_tag="ACHY3M_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4319672.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         1224 a         1224 c         1076 g         1297 t
ORIGIN      
        1 aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta ctctggctac
       61 tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtc agcgtcatga
      121 gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca cgagtgggaa
      181 caacgttagc aaccgcgtta tctaaatcga cgttatcagc cagggtattg gtatccagcg
      241 ccactctatt ttcccgatat tcagtggcat aaggcagcac ggcataacca cgccagtcgg
      301 tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct ttaacaagca
      361 ccactgtttc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc aataccccac
      421 cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg atattggcat
      481 tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt acttccgcta tttccatcgc
      541 ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc agcaacgtac
      601 cgtatacacc agccagattg gtcatccgac cattgagatc gtgtgacatg ctgtagctgg
      661 cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg aaaggaatat
      721 tgacgttaag cgctaacatc tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat
      781 agctgagcgt ccagttgata tcttcgaatg cagtatttaa accagcctgg aattgctcat
      841 cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt gttgatgtgc
      901 gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa gcgaggttgt
      961 aatagtcggt gaatttcggc ttaacctgaa taactccgtc ctgtgtttcg atgttgtagc
     1021 cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc gaataacggt
     1081 aacccactaa ctgaatattc gtgcctgact cattgagcga tttgttatag agaaaacgca
     1141 ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca
     1201 cagacagagc gcccagtgcc cccatgtttt tcccgatacc gaaattaaaa gcacgataac
     1261 gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg tggagtaatg
     1321 tactctggaa aaagcggggt ttttcctgct gcgcatttcc actacggtat tctcctgccg
     1381 taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg
     1441 taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca ccactattac
     1501 ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta ttataaatgt
     1561 catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg tggatcaccg
     1621 gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc
     1681 gaaagttaat accatcgaaa atatcgccct gagtataacc atcacccagc gtcagccggg
     1741 aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat ttatttttgc
     1801 tacctgatga tctgtcgcta cggttataac tccaggtggt attgtcgcgt aaacgccacg
     1861 caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta cccccaatcc
     1921 gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta ataccgggat
     1981 cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc tgagggatcg
     2041 tcaggttgag tcgctgctga ccaacatcta aatgcgcagt agcgtcatgg atcatggagg
     2101 ttaatggcac gcaggcatca tccgccagca gattcatacc ggagacagaa gccgtattca
     2161 gccccatact ggcaagttgc gcgcgtgtca ggcagggaac aatcccttgt tcgctgtcgc
     2221 ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag atatcgacac
     2281 gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatca gccacagcct
     2341 ggggatcatc cgctaaaaag cgcggattaa aatagagttc ggcagatgac aaaggtgtct
     2401 gtgcggcaaa agcacaggcg acaaagagcc ggacaaaaaa accagccaaa cgatgcttac
     2461 gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa attcagatat gacattattc
     2521 ctccggttta aaatcacccg gcagtcaatt ctttttattc aggcgaaaaa ttccccctgc
     2581 gttattccat tacgcccgtc attttggggg taagtgcgcc ataatcattt attgttcggt
     2641 aagtaatatt gcttcccgca tcagaaggca atttaaccgt gctttcgccc attggaggca
     2701 ccaaggcatt ttcaagaacc cgggctccgg cattcaactc tgttaccgtc aggtaatagg
     2761 gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg aaatcttaat ttttctgcgg
     2821 cctgatcggg tggcaacgct aatttagccg gacgatagta cagtttaatg cggctgataa
     2881 ttgcgagctg tagcgtattc tcagtcaatt ttgatttatc cattgacgga atcgctttaa
     2941 cgttcatcca gaataaactt tcccggtcct gtggcaattg gttatttgtt gcatcaagaa
     3001 tacgcaaggt attctctttt tttcccttca tcgcaaacag aggaggcgtc acgataaaac
     3061 gaccatcctt tacatcatcg gcattttcca cccatgattg aattaaatag gtactatttt
     3121 catcattatt tgtcacggca agttgcactt gtttttgccc tgccggataa attacgcgag
     3181 tcgcacctaa ggccactccc gcttcagcgc gtccggcaac catcattgcc atgaacatca
     3241 ggatacctgc cagcaagcag aatgttattt cctgcgattt ccttacattg acgtttttat
     3301 tactcactgt cctgttcctg ttatcacatt atctgctgaa caattactga taggttaaag
     3361 agaaccaggc ctgggcattg gcgatgccgc cagtaacccg acgcccggta gcacgatatt
     3421 tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg tttccagttt gctggaggac
     3481 gattaatcgg tacgaggttt ccttcatcat caaacaacgc tacgccaata ttggtggcta
     3541 tccctggccc ctctcccacg gaaagcacat ccggattttt accatccgcg acaccgtgaa
     3601 acgccacacc tacacgttcg ctcaccaccg tgctacattc ccgtaaatga ataataaaag
     3661 gcaccggtgc gctatcttcc cctaccgcat gaaaccggtt actgctgatt tgccccatat
     3721 tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt ttccgcaata atgacgccct
     3781 gaaattgcat atttccgccg ggcaacgtgg tattccattt atttccggcc agagcaaaca
     3841 ttggcagcaa cgacgctaat agaaataatc tcttcctttt catcgttatt tttatcgcac
     3901 aagggtgggc atccctgccc gtaatgacgt ccctgaacct gggtaggtta ttgatactga
     3961 accttgaagg tcgcatccgc attagcagca cccggggttg cggccccggt tgcaaaataa
     4021 cgcgcctgga acggaatggt attggttccg ttattcaggg ttgtttctga actaaatgtc
     4081 gcaccgtcca gcgccagcgc agcacccgtt ctgtccagga tctgcacacc aacgtttgtt
     4141 gcgctacccg cagcagaact ctgcagagcc agtacatcag tatgcgtagc atcaattgcc
     4201 gtacctaaga aggcaacagc ggcttttgtg gcaacagtgg tatcgcaatc attcagctga
     4261 atgttaaaac caacggcaga gctggttgct ccagcctgct tcaggctagc ggtacgaacc
     4321 tggcccaact gaacggtttg atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca
     4381 acttcccctt taaagtgaac ggtcccacca tttaccgtcg tagtatcggc cagagccgct
     4441 gcggaactga gggacagagc cgacagaaca acgattgcca gagttttaat tttcatgctg
     4501 ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta cactttgttt
     4561 tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga aacatttggg
     4621 gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta ttcgttggct
     4681 taatattcta ttgttatctt tatttataga tttttatatt gcatgaggtg gtttttggag
     4741 agaagaatga ggaagatgcg tcgagccaca gaaacgttag ctttacatat agcggaggtg
     4801 atgtgaaatt aattcacaag a
//
LOCUS       DAZUFB010000207         4781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZUFB010000207 DAZUFB010000000
VERSION     DAZUFB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.207"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..165)
                     /locus_tag="ACHY3M_004944"
     CDS             complement(<1..165)
                     /locus_tag="ACHY3M_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319673.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDE"
     gene            complement(158..412)
                     /locus_tag="ACHY3M_004945"
     CDS             complement(158..412)
                     /locus_tag="ACHY3M_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319674.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(409..831)
                     /locus_tag="ACHY3M_004946"
     CDS             complement(409..831)
                     /locus_tag="ACHY3M_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS4319675.1"
                     /translation="MAKVFTQEEQEKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(872..1834)
                     /locus_tag="ACHY3M_004947"
     CDS             complement(872..1834)
                     /locus_tag="ACHY3M_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319676.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(1857..2282)
                     /locus_tag="ACHY3M_004948"
     CDS             complement(1857..2282)
                     /locus_tag="ACHY3M_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4319677.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(2279..2581)
                     /locus_tag="ACHY3M_004949"
     CDS             complement(2279..2581)
                     /locus_tag="ACHY3M_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4319678.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSSSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            2679..3050
                     /locus_tag="ACHY3M_004950"
     CDS             2679..3050
                     /locus_tag="ACHY3M_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319679.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            3071..3262
                     /locus_tag="ACHY3M_004951"
     CDS             3071..3262
                     /locus_tag="ACHY3M_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFS4319680.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            3264..3542
                     /locus_tag="ACHY3M_004952"
     CDS             3264..3542
                     /locus_tag="ACHY3M_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4319681.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            3895..4196
                     /locus_tag="ACHY3M_004953"
                     /pseudo
     CDS             3895..4196
                     /locus_tag="ACHY3M_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4268..4486
                     /gene="ydfC"
                     /locus_tag="ACHY3M_004954"
     CDS             4268..4486
                     /gene="ydfC"
                     /locus_tag="ACHY3M_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFS4319682.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT         1264 a         1232 c         1066 g         1219 t
ORIGIN      
        1 ttcatctctg aagcagagga cgtattgaag attattttcc tccatccagg cgttaaactc
       61 ttccgttgaa atatattccc gaccgtcaca gaatttttca tattcaggat gcgtccagca
      121 gccatattca tcacgtacta ctggtatttc tttaatttca ttcatttctg ttctcccacg
      181 ttttcagact ttcaccacag aacggacaaa atgaaacccg aactggtaat ttagaaaatt
      241 caccggaacg caacatcaca aaatcaggac cgcgagttaa actctcattc cagattttgt
      301 atatcagcag accttttcgc atcgtgtatt cagcatcatg ctcaagggac tttgccagtg
      361 ctgcacatgg ttctatcttg ttgccattaa cctggcattt tgattcactc accgcaccac
      421 ctcctcaaaa ttcccctgat aaaacgccag tacgcgctgc ataacttcgc tcttccggca
      481 ctcgagacag attatgttca gacgcctgtc gtagcgacgt atttctccgt caggtaatga
      541 ccagataagg tccggatcaa ccgcagatgg tttcttcagc tttgcccttg agagcttttt
      601 acgggcattt tgccagtcct tacgcgcctg ttcagacggg aataacccgt aaccagagtt
      661 gtatacatcg ccactggcaa ccagctctct ggccagaacg ctcatcagat atcttgttgc
      721 cccagtttta gtttccagtt gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac
      781 aacctgccct ttaattttct cctgctcttc ttgtgtaaaa acttttgcca caagccctcc
      841 tgaaaattac ctcatgacca gaaattaaca cttaccccct gaagcccggc ggaatttcag
      901 tgtccggttc agaaatgtga ttcacgcaac gctgcgcagg cgaacgcccc aggcggataa
      961 ccagttcatc ccatttttcc cggagttttg ccggactcat gatgtttttt acccagaacg
     1021 aatcccgctg gagacgccca aacatttcac aaatttgtct gtgagttctg ccatccagca
     1081 tccgcattgt gcgaacgtca ttggcccatg ctgtccagtt gggttctttc ggtctagtga
     1141 tctcgccatc atcgctggcc gcctgctcgt aaagactcac gattcgtccc cagatccact
     1201 gtgcgcacac caaatcttcc tgacttcccc actggcgttt tttcgcactg aacacaaccg
     1261 cgtcagggtg tcgggttaaa aaatcctgtt cagccgtctg cgggtccggt tgcgaagcgt
     1321 ccggacaaga agatctttta tctgacggat caggttttaa tactgacgga tcggggtcaa
     1381 tcatcgcccc cctaatcggc agttttttat caacagttga tccatcaaaa tttgacgggt
     1441 caaccgttga ggggtcaata tttgacgggt caactgttaa cgggtcattt tttgccgggc
     1501 taatttttct tttcggttta tatgactcac gcgccgccgc agcagctgct tcgagttttt
     1561 ccacattaag ccgatagata ttgcttacat tacgcccacc gaccttacgc tcttccttcg
     1621 tcagccagcc ctctttcgcc agttctgcaa tagccgattt cactgtggat tcacttcttg
     1681 caccgatctg acgccggata gtttcaatgg caggccatga cacgccctcg tcattgctgt
     1741 agtctgcaag acgggccata accgccaccc tggataagat catgccggtg aaggcgcacc
     1801 cttcccagac aagaccatga agcttgctgc tcataaaacc cccgaacacc gtgcttttag
     1861 tgcatcacca cagcattccc tgccgggccg ccgcgattca tctggtcata caaaacaacc
     1921 gctgacgcaa caaaatcatc gacatccttc accagccgat ccctccgttc gacgatctca
     1981 cggtaatatt cagaactgtg gctgcgcata cgggccacca gcaaaggcgg catcgccttt
     2041 tcgatcgccg gtaacagagc ctgcattttt tcaacagcat caggggtgtc tttatccagc
     2101 caacggaaaa ttttctgggt attacgggcc agggcttccg gatggctgtc gtcatacagt
     2161 tccgggaacg tcattcccag ctcgaaatac gctttggtaa ttttcgcagc cggtactttt
     2221 tcgccgtccg gatgcgccca gacattcatc gccatgcgga tgtgttcatg cttgattttc
     2281 atgaatcaac tccatcagat aagcatgcac tacaatcacc ttcagcatga actacatgtg
     2341 tttgccccaa acgaatgccg ctcgcatact caggccaaat aagctcccaa tcatggggtc
     2401 gtagctccgc cctacttact tggccttccg tcgcagattc gatcataagg gcgcgggttg
     2461 gagatatagc tgttcgtcca gacgccattt gcgataagta agatgacgat acaccaagtc
     2521 tggccgcgaa tttcttagca tcaccaaccc ttaatgattt aataaactct tttaatgtca
     2581 taccttcctc ggtttagtgt ttttttgcga gtttagtgtt taataaacca ttaagtcaag
     2641 tatttgcttg tttagtgatt actaaagata attaccacat gcaaaaaaaa gaaattcgcc
     2701 gtttacgtct caaggagtgg tttaaagata aaactctgcc acccaaagag aagagctacc
     2761 tatctcaact aatgagtggg agagcctcgt ttggagaaaa agctgccaga agaatagagc
     2821 aaacatacgg gatgccggaa gggtatctgg atgcggaata cgcagaacaa ccgggggttt
     2881 ctccaccaca tgcagggtta acgtctaatc aactggaatt attgcagatt ttttcagcct
     2941 tccctgagga tgagcaacgc cagataatca gcgagttaaa gcagaaaaaa gaatcaatgg
     3001 aagatctcat agcgagatgg attgcggcgc aaaaatgccg ccgcgcctga gttataaaac
     3061 cggaggaaac atgaatagag ccctttcacc aatggtttct gaatttgaaa ccattgaaca
     3121 agaaaacagt tacaacgaat ggctgcgtgc gaaagtagca acgagccttg cagatccgcg
     3181 cccagcaatt ccccatgacg aagttgagcg cagaatggca gaacgctttg ctaagatgcg
     3241 caaggaacgg agcaagcagt aaaatgttac ccgtgttatg gcttgaaagc gcagataccg
     3301 acctagatga tataactagt tatattgctc gtttcgacat agatgcggct gaacgcttat
     3361 ggcagcgatt aaggggttgt gtgctgccgt tatccgaaca tccgtattta tacccaccaa
     3421 gcgacagagt acctggcttg cgtgagattg tagcccaccc taactatata attctatacc
     3481 gcgtaacaac atcaagcgtt gaagtagtaa acgtgatcca cgcaagacgc cagtttccct
     3541 aactttcact accaatagaa acataacaac cgcaacgact ttatcaaaag cgttgtgttt
     3601 gttatgtccc gcggtttagt ttttacttga cttaagttta atgtttatta aactaaaaat
     3661 accaacccac cccgccccac agaacgcagg gaaatacttc gagttacccg gcagtggtca
     3721 ggggttaagt agccagcccg aggcgtatga acatgacggc agggttcaac tttaataact
     3781 atgcagcagg tttttgttcc gctaccccgg cgttaagggg aaacagaggg tttctcagtg
     3841 ggcgaagtca aacatcagaa tggaaggcat cccgggatcg gcaaagaagc agcaatggcg
     3901 ctttatattg acatcagcgc cattgcagga caggtaagag ttatcagagc ggtaactaag
     3961 cggtatgcgc ctttacttca gaaagtctct ggtgagtgca ccgaagatat tgtcaacgat
     4021 ttcgtcatcg aactgcgagg actcatcttc agtttacaag gtgaaccaca atttttgcag
     4081 atggctcccg cgaaactgtc agagccctgc ggcttaaagg atgtgtcaaa gacttcgcca
     4141 ccacattctg ggcaagaaaa cttgattgta ttcataacca atttcctctc gagtaacaga
     4201 cccctcagag gataccacct cgcctgacgt ggttaaaagc aggcaacgct aaccacaagg
     4261 agccgacatg cagaaacgag aacccgtcat catcgcgcca gactataccg atgatgaact
     4321 ttatgagtgg atgcaccaga aaattaatgc agcgcaggat ctgaaatggg ccaatgaagc
     4381 cagggctaag caggctgaaa atctgtccgc tctggagcag gatatcacca atctggaaaa
     4441 agcagcggca ttaagcattg ccagaatgat tacatacccg cgttaatagc taaccaacga
     4501 agctaaggtt ggtaattaag gagttctcca cgggtgaggt ggagtgcgtg cgccggacac
     4561 gggtgagcat acggcactga cagtttactg aaaggatatt tccctgaaaa gtcagaccat
     4621 aacgcgaaat ggcgcggcgg taagtatgga ggggttattc cttcccccgt tgaggacacc
     4681 gggttgtcag gttgaccata cgcttaagtg acaaccccgc tgcaacgccc tctgttatca
     4741 attttctggt gacgtttggc ggtatcagtt ttactccgtg a
//
LOCUS       DAZUFB010000208         4772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZUFB010000208 DAZUFB010000000
VERSION     DAZUFB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.209"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..90
                     /locus_tag="ACHY3M_004955"
     CDS             <1..90
                     /locus_tag="ACHY3M_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4319683.1"
                     /translation="DDGGSCHLWLGRGVPPAVNRRRWKGDVWP"
     gene            81..659
                     /locus_tag="ACHY3M_004956"
     CDS             81..659
                     /locus_tag="ACHY3M_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319684.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            656..1051
                     /locus_tag="ACHY3M_004957"
     CDS             656..1051
                     /locus_tag="ACHY3M_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4319685.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1059..1799
                     /locus_tag="ACHY3M_004958"
     CDS             1059..1799
                     /locus_tag="ACHY3M_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319686.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            1815..2237
                     /locus_tag="ACHY3M_004959"
     CDS             1815..2237
                     /locus_tag="ACHY3M_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4319687.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2219..2653
                     /locus_tag="ACHY3M_004960"
     CDS             2219..2653
                     /locus_tag="ACHY3M_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4319688.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2646..>4772
                     /locus_tag="ACHY3M_004961"
     CDS             2646..>4772
                     /locus_tag="ACHY3M_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4319689.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQECLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIG"
BASE COUNT         1117 a         1185 c         1555 g          915 t
ORIGIN      
        1 gatgatggcg gaagctgtca tctctggctt gggcggggcg taccgcctgc cgttaaccgt
       61 cgccgctgga agggggatgt atggccataa aaggtcttga gcaggccgtt gaaaacctca
      121 gccgtatcag cagaacggcg gtgcccggtg ccgccgcaat ggccattaac cgcgttgctt
      181 catccgcgat atcgcagtcg gtgtcacagg ttgcccgtga gacaaaggta cgccggaaac
      241 tggtaaagga aagggccagg ctgaaaaggg ccacggtcaa aaatccgcag gccagaatca
      301 aggttaaccg gggggatttg cccgtaatca agctgggtaa tgcgcgggtt gtcctttcgc
      361 gccgcaggcg tcgtaaaaag gggcagcgtt catccctgaa aggtggcggc agcgtgcttg
      421 tggtgggtaa ccgtcgtatt cccggcgcgt ttattcagca actgaaaaat ggccggtggc
      481 atgttatgca gcgtgtggcc gggaaaaacc gttaccccat tgatgtggtg aaaatcccga
      541 tggcggtgcc gctgaccacg gcgtttaaac aaaatattga gcggatacgg cgtgaacgtc
      601 ttccgaaaga gctgggctat gcgctgcagc atcaactgag gatggtaata aagcgatgaa
      661 acatactgaa ctccgtgcag ccgtactgga tgcactggag aagcatgaca ccggggcgac
      721 gttttttgat ggtcgccccg ctgtttttga tgaggcggat tttccggcag ttgccgttta
      781 tctcaccggc gctgaataca cgggcgaagc gctggacagc gatacctggc aggcggagct
      841 gcatatcgaa gttttcctgc ctgctcaggt gccggattca gagctggatg cgtggatgga
      901 gtcccggatt tatccggtga tgagcgatat cccggcactg tcagagttga tcaccagtat
      961 ggtggccagt ggctatgact accgacgcga cgatgatgcg ggcctgtgga gttcagccga
     1021 tctgacttat gtcattacct atgaaatgtg aggacgctat gcctgtacca aatcctgtaa
     1081 tgccggtgaa aggtgccggg accaccctgt gggtttataa ggggagcggt gacccttatg
     1141 cgaatccgct ttcagacgtt gactggtcgc gtctggcaaa agttaaagac ctgacgcccg
     1201 gcgaactgac cgctgagtcc tatgacgaca gctatctcga tgatgaagat gcggactgga
     1261 ctgcgaccgg gcaggggcag aaatcagccg gagataccag cttcacgctg gcgtggatgc
     1321 ccggagagca ggggcagcag gcgctgctgg cgtggtttaa tgaaggtgat acccgtgcct
     1381 ataaaatccg cttcccgaac ggcacggtcg atgtgttccg tggctgggtc agcagtatcg
     1441 gtaaagcggt gacggcgaag gaagtgatca cccgcacggt gaaggtcacc aatgtgggac
     1501 gcccgtcgat ggcagaagat cgcagcacgg tcacagcggc aaccggcatg accgtgacgc
     1561 ctgccagcac ctcggtggtg aaagggcaga gcaccacgct gaccgtggca ttccagccgg
     1621 aaggcgcaac cgacaagagc ttccgtgcgg tgtctgcgga taaaacaaaa gccaccgtgt
     1681 cggtcagtgg tatgaccatc accgtgaaag gtgttgctgc aggcaaggtc aacattccgg
     1741 tcgtatccgg taatggtgaa cttgctgcgg ttgcagaaat cactgtcacc gacagttaat
     1801 ccggagagtc agcgatgttc ctgaaaaccg aatcatttga acataacggc gtgaccgtca
     1861 cgctttctga actttcagcc ctgcagcgta ttgagcatct cgccctgatg aaacggcagg
     1921 cagaacaggc ggagtcagac agcaaccgga agtttacagt ggaagacgcc atcagaaccg
     1981 gtgcttttgt ggtggcgatg tccctgtggc ataaccatcc gcagaagaca aagcagcctt
     2041 ccatgaatga agccgttaaa cagattgagc aggaagtgct taccacctgg cccacagagg
     2101 caatttctca tgctgaaaac gtggtgtacc ggctgtccgg tatgtatgag tttgtggtga
     2161 atgatgcccc tgaacaggca gaggacgccg ggcctgcaga gcctgtttct gcgggaaagt
     2221 gttcgacggt gagctgagtt ttgccctgaa actggcgcgc gagatggggc gacccgactg
     2281 gcgtgccatg cttgccggga tgtcatccac ggagtatgcc gactggcacc gcttttacag
     2341 tacccattat tttcatgatg ttctgctgga tatgcacttt tccgggctga cgtacaccgt
     2401 gctcagcctg tttttcagcg atccggatat gcatccgctg gatttcagtc tgctgaaccg
     2461 gcgtgaggct gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg cagggcttgc
     2521 cggaggcgtc cgctttggcc cggacgggaa tgaagttatc cccgcttccc cggatgtggc
     2581 ggacatgacg gaggatgacg taatgctgat gacagtatca gaagggatcg caggaggagt
     2641 ccggtatggc tgaaccggta ggcgatctgg tcgttgattt aagtctggat gcggccagat
     2701 ttgacgagca gatggccaga gtcaggcgtc atttttccgg tacggaaagt gatgcgaaaa
     2761 aaacagcggc agtcgttgaa cagtcgctga gccgacaggc gctggctgca cagaaagcgg
     2821 ggatttccgt cgggcagtat aaagccgcca tgcgtatgct gcctgcacag ttcaccgacg
     2881 tggccacgca gcttgcaggc gggcaaagtc cgtggctgat cctgctgcaa cagggggggc
     2941 aggtgaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc ggtgcgatca
     3001 ccctgccgat ggtgggggcc acctcgctgg cggtggcgac cggtgcgctg gcgtatgcct
     3061 ggtatcaggg caactcaacc ctgtccgatt tcaacaaaac gctggtcctt tccggcaatc
     3121 aggcgggact gacggcagat cgtatgctgg tcctgtccag agccgggcag gcggcagggc
     3181 tgacgtttaa ccagaccagc gagtcactca gcgcactggt taaggcgggg gtaagcggtg
     3241 aggctcagat tgcgtccatc agccagagtg tggcgcgttt ctcctctgca tccggcgtgg
     3301 aggtggacaa ggtcgctgaa gccttcggga agctgaccac agacccgacg tcggggctga
     3361 cggcgatggc acgccagttc cataacgtga cggcggagca gattgcgtat gttgctcagt
     3421 tgcagcgttc cggcgatgaa gccggggcat tgcaggcggc gaacgaggcc gcaacgaaag
     3481 ggtttgatga ccagacccgc cgcctgaaag agaacatggg cacgctggag acctgggcag
     3541 acaggactgc acgggcattc aaatccatgt gggatgcggt gctggatatt ggtcgtcctg
     3601 ataccgcgca ggagatgctg attaaggcag aggctgcgtt taagaaagca gacgacatct
     3661 ggaatctgcg caaggatgat tattttgtta acgatgaagc gcgggcgcgt tactgggatg
     3721 atcgtgaaaa ggcccgtctt gcgcttgaag ccgcccgaaa gaaggctgag cagcagactc
     3781 aacaggacaa aaatgcgcag cagcagagcg ataccgaagc gtcacggctg aaatataccg
     3841 aagaggcgca gaaggcttac gaacggctgc agacgccgct ggagaaatat accgcccgtc
     3901 aggaagaact gaacaaggca ctgaaagacg ggaaaatcct gcaggcggat tacaacacgc
     3961 tgatggcggc ggcgaaaaag gattatgaag cgacgctgaa aaagccgaaa cagtccggcg
     4021 tgaaggtgtc tgcgggcgat cgtcaggaag acagtgctca tgctgccctg ctgacgcttc
     4081 aggcagaact ccggacgctg gagaagcatg ccggagcaaa tgagaaaatc agccagcagc
     4141 gccgggattt gtggaaggcg gagagtcagt tcgcggtact ggaggaggcg gcgcaacgtc
     4201 gccagctgtc tgcacaggag aaatccctgc tggcgcataa agatgagacg ctggagtaca
     4261 aacgccagct ggctgcactt ggcgacaagg ttacgtatca ggagtgcctg aacgcgctgg
     4321 cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggccgcc attgatgcga
     4381 aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag cgcctgaagg
     4441 aacagtatgg cgataatccg ctggcgctga ataacgtcat gtcagagcag aaaaagacct
     4501 gggcggctga agaccagctt cgcgggagct ggatggcagg cctgaagtcc ggctggagtg
     4561 agtgggaaga gagcgccacg gacagtatgt cgcaggtaaa aagtgcagcc acgcagacct
     4621 ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgagcag aactggcgca
     4681 gcttcacccg ttccgtgctg tccatgatga cagaaattct gcttaagcag gcaatggtgg
     4741 ggattgtcgg gagtatcggc agcgccattg gc
//
LOCUS       DAZUFB010000209         4765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZUFB010000209 DAZUFB010000000
VERSION     DAZUFB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.210"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(8..439)
                     /locus_tag="ACHY3M_004962"
     CDS             complement(8..439)
                     /locus_tag="ACHY3M_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS4319690.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(609..685)
                     /locus_tag="ACHY3M_004963"
     tRNA            complement(609..685)
                     /locus_tag="ACHY3M_004963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Arg,seq:tct)
     gene            complement(786..861)
                     /locus_tag="ACHY3M_004964"
     tRNA            complement(786..861)
                     /locus_tag="ACHY3M_004964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Ile,seq:cat)
     gene            complement(1001..1555)
                     /locus_tag="ACHY3M_004965"
     CDS             complement(1001..1555)
                     /locus_tag="ACHY3M_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4319691.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1552..1842)
                     /locus_tag="ACHY3M_004966"
     CDS             complement(1552..1842)
                     /locus_tag="ACHY3M_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS4319692.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(1842..2441)
                     /locus_tag="ACHY3M_004967"
     CDS             complement(1842..2441)
                     /locus_tag="ACHY3M_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS4319693.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
     gene            2941..4332
                     /locus_tag="ACHY3M_004968"
     CDS             2941..4332
                     /locus_tag="ACHY3M_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4319694.1"
                     /translation="MTENTTPTADFSPSKRFFVSMLTRDIDLNDAILDLLDNCVDGAL
                     RTIKDTKKTSKPYEGFYAKLTINKDVFIIEDNCGGIPKSFREYAFKMGRPHQKEEENE
                     GTVGVYGIGMKRAIFKMGRDCSIQSNNPDGAFTVDITPDWIDGDGWKIPMHESDYDNK
                     NPTGTTIEIKKLHSNVAQKFNESTYLTDLFLQIKHSLSFIIQKGFKIELNGVVVEHNP
                     INIITDHSKIEPYIYKAKIDDVDIDLVVGFYKNLEDDNDDVLEKRSSDDAGWTVICND
                     RVVLYCDKTHLTGWGFANVPRFHTQFIAISGVVRFTSKNPEKLPITTTKRGVDLSSTL
                     YNDVRNKMIEGMMHFIRFTNQWKGEHLEEGKKLLKSAQSHEAQSLFEITPQSTPEDKK
                     NNWSNPNRNKNEWRYTPKLPTPVKKSSTVRIIFTREKEDVKILSKYFFGHEDASASDV
                     GMQCFDTVIEEVK"
     gene            4332..>4765
                     /locus_tag="ACHY3M_004969"
     CDS             4332..>4765
                     /locus_tag="ACHY3M_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4319695.1"
                     /translation="MSGQYIPYHLRHNKSIDREIFLESLNLLSKRLNIQEYTYIGFGG
                     PMLEDFRIMHNRIALSDMISLEEQESTHIRQKYNLPYNCIDCKLISAHDFILDYSFSK
                     PSITWLDYASPKKIQTDLDDIHLLSTKVSSFDILKVTFPINP"
BASE COUNT         1401 a         1097 c          959 g         1308 t
ORIGIN      
        1 tttaccgtca caggtgattc tccagacgaa gacccagaac accggcaatc tcttccagca
       61 ccttgcgctc ttccggctca atttcgccgt ctgcctccgc aatggccacc gccacatcca
      121 gcacatcttc cgcttcacgc gtatcgtgtt tcacatcctc aatctcgcgt aacgcggcac
      181 gacgaccaat tttaaaattg gtatccagct gaccgataat agttgcgcta atcgcattaa
      241 tttccgaggt aaacgcagac aacgcaggct gattacgtaa gacctgttcg atcttcgctt
      301 tctcggatgc ctcgcattca ccatctgcat aggccaccag gtatgcagcg ttaatcaccg
      361 cctgtgccag atcgcgtttc tcaaactttt taatttccgc tgccgctctg cgggttttct
      421 ttttgaagat tccaaacatc gtgacgttcc tttgggtggg tgagccaacg cccgggagcg
      481 atctgcccac agagaaagtc acactgacca ctccgtaagc tcacccccga aaggctctgt
      541 ggttgatatg cgccgggcgt ggcgcagata caaaaaaggc ccgccgaagc gagcctggaa
      601 aataagtgtg gcgcgttgta gtggagtcga accactgacc gattgcttag aaggcaattg
      661 ctctgtccgg ctgagctaac aacgcagaat accgataatg gaccgccatc ggggacccgc
      721 ccccgcacca acaaccctgt tatggtgtcg tctgctcttc ctgataagct aatggcggtt
      781 tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc
      841 tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta attccgcaat
      901 aaaaaacccg ctcaatggcg ggttctggta aagttcatgc gtttggttcg cctcgcgata
      961 cagctttgcg aagcttaccg gaattgaagc agtttttacg tcaaaaagca ataacttttt
     1021 tctctatacc aaaagccata accattggtt tgtacaaaat aaattctgcc acctttagcc
     1081 aatgctcaat gcgtctttca caggttctta aactccattc cggatgtgca tcattcagca
     1141 gttcagccat tttgcgctta gtcatccccc gccccacata acgctgactc agaacattga
     1201 gcagcccggg ataacctgcc agtacttcac caataaccct gtcgattatt aacgcctctg
     1261 aatcggtaca atgcaccagc cagctttttt gcttgccgtt gatcatatcc cgcaaaaaag
     1321 cctcaagttt aggtttgtcc agacctgctt ttttcatcct ccggagcgcc tcgttaattg
     1381 ccgtttttgt cagcttttta gaggtcagca actggttgaa catatttccc gtcttaccgc
     1441 cgccaatata cgaccagcgc ccccacatac gtagtttccc ctgaatccag acactttcca
     1501 gcgtggtgag acgaaggtgc tctccgcttt ttcctgtgtt cgttgggtaa atcataaata
     1561 accgtccttt ctccagatct cttgtgtgcg aaaaacacct tctgcatgca tcaggcgcaa
     1621 ttcttctttg gtgtaatcgc tggtttttac tctcccgtcg attaaatcgt ggcatgagtt
     1681 acaggcaatc gctgcctgca tatcgtgtgg ctttatcgct gttccgcacg ttcccgccag
     1741 cctgtaatgc gccagcacag aggtttcggg attgtgattg cagtagccag ggattctgat
     1801 ctggcatatc tggcctttag ccgctttacg taaattcacc attacgcaaa ctccagtagt
     1861 tgtgcggcca cattttcaac ttcctcctga gaggagaatt tacggaacag aatccagttc
     1921 cacagcacat tcagtacaga tttataaacc tgctgaaact cgacttcgtc catattcgca
     1981 aaagcgatgg attttgcccg acgcccacgg ctaccgtccg gataaatatg ctcggtgtaa
     2041 aatccggcct gaatggttac ccactcgcgg aaagcctcaa acgactttag caacgccgta
     2101 tcccgggttc tacgagtcgc aacggtgtta aggtattgct ctgcggcatc actcagggct
     2161 ggcgtgtgtt cccgaccaac tgattcgcac aggtaatcaa cgaaaccaga cagcagttct
     2221 cgttcgcgag gcgtgatcgc cccaccgacc ggagtccagt aatcgaatcc cagttgcagg
     2281 agtttgaaaa aacgcttgtg gaatgcgtag ttacgcacac gcttaaaatc tgcgtgtatc
     2341 cactcaccta ttttgatttg atgcagaaaa tcgcaactct ccggcgtcgc cgggagaagt
     2401 aaaccagaag aagtttgttt gaccagttgt atatgcgcca tttctcaatc tctcgatggc
     2461 gcagtgcagc agatgccagt tgttcaagct agcacgcaaa ttgtaaacca gaatgccagg
     2521 aaaaaacaaa acccgccgaa gctggttgtg tgcggttgcg ttgaagtctc ctagccaaag
     2581 agtagcggtg accactcccc gccgataatc ttagttgttt aatttcgtaa gctaactagg
     2641 ctaaaagctc tctgttgtct gcctgttgta caattaacaa aatgaacaac gaactagata
     2701 taccaacgcc agaatggtgc aacaaatccc aaacaacaca gcaatgcaac aataacatat
     2761 gttattttcc tctcacggga ttcagtcctg aattctcata aagctaatct ttttttaaaa
     2821 acatactgtt agatcacatg cactttcttt cttaacatcg ttgacttcgc atgcgaagcg
     2881 tttaaaatca acaatttgca cttaaggtat ttattttccc aaacacatag agagtaaatt
     2941 atgactgaga acacgactcc aacagcagac tttagtccta gtaaaagatt ttttgtaagt
     3001 atgctaactc gagatatcga cttaaacgat gccatacttg atctgcttga taactgcgtc
     3061 gatggggctt tacgtacaat aaaagacaca aaaaaaactt caaaaccata tgaaggattt
     3121 tatgcgaaac ttacgattaa taaagatgtc tttattatag aagacaattg cggaggtatc
     3181 cctaaaagct tccgagaata tgcttttaaa atgggacgac cccatcaaaa agaagaagaa
     3241 aatgaaggca ccgttggcgt ctatggtatt ggtatgaaaa gagccatttt taaaatgggt
     3301 agagattgct caatacaatc gaataatccc gatggcgcat tcactgtaga tataacacct
     3361 gattggatcg atggtgatgg ttggaaaata ccgatgcacg agagtgacta tgataacaaa
     3421 aatccaacag gcacaactat agagataaaa aaactccatt ctaatgtggc acaaaaattt
     3481 aatgagagca catatcttac tgatctcttt ttacagataa aacactcatt gtcatttatt
     3541 attcaaaaag gatttaaaat agaattaaat ggagttgttg ttgaacataa tccgattaac
     3601 atcataactg atcattctaa aatagaacca tacatatata aagctaaaat agacgatgtt
     3661 gatatcgatt tagttgttgg cttctataaa aatttagaag atgacaatga tgatgtttta
     3721 gaaaaaagga gttctgatga tgctggatgg actgtcattt gcaatgaccg cgttgttcta
     3781 tattgtgata agacacatct cacaggttgg ggctttgcta atgtaccaag gtttcatacc
     3841 caatttatag caatttctgg ggttgttcgc tttaccagta aaaacccaga aaaattacca
     3901 ataacaacaa cgaaacgagg tgtcgatctt agttctacat tgtacaatga tgtacgaaat
     3961 aaaatgattg aagggatgat gcattttatt cgattcacca atcagtggaa aggtgagcat
     4021 cttgaagaag ggaaaaaatt acttaagtca gctcagtcac atgaagctca atctttattt
     4081 gaaattaccc cacaatcaac tccagaagat aaaaaaaata actggtccaa ccctaataga
     4141 aacaaaaatg agtggcgata tacacctaag ctgcctaccc cagtaaaaaa aagctctact
     4201 gtacgaataa tcttcaccag agagaaagaa gatgttaaaa ttttatctaa atatttcttt
     4261 ggacatgaag atgctagcgc ttctgatgtg gggatgcaat gctttgatac ggtgattgaa
     4321 gaggtgaagt gatgtctgga caatacattc cctatcactt aaggcataat aagtctattg
     4381 atagagagat ttttctcgaa agcctaaacc tcttaagcaa aagactaaat attcaagaat
     4441 atacatatat aggttttggt ggtccgatgc ttgaagattt tagaatcatg cataatcgta
     4501 tcgctctttc tgatatgatt tctctcgaag aacaagagtc tactcacatc agacaaaaat
     4561 ataatttgcc ttataattgt attgattgta agctaatatc agctcatgac tttattcttg
     4621 attatagttt ttctaaacca tcaataacat ggttagacta tgcctccccg aaaaaaatac
     4681 aaacggatct agatgatata cacttactaa gtactaaagt aagctctttt gatattttga
     4741 aggtcacctt ccccataaat cctag
//
LOCUS       DAZUFB010000210         4671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZUFB010000210 DAZUFB010000000
VERSION     DAZUFB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.211"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..789
                     /gene="paa"
                     /locus_tag="ACHY3M_004970"
     CDS             31..789
                     /gene="paa"
                     /locus_tag="ACHY3M_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFS4319696.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1125..1280
                     /gene="hokD"
                     /locus_tag="ACHY3M_004971"
     CDS             1125..1280
                     /gene="hokD"
                     /locus_tag="ACHY3M_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4319697.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1501..1758
                     /locus_tag="ACHY3M_004972"
     CDS             1501..1758
                     /locus_tag="ACHY3M_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319698.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1828..2106
                     /locus_tag="ACHY3M_004973"
     CDS             1828..2106
                     /locus_tag="ACHY3M_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319699.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGDEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2108..3163
                     /locus_tag="ACHY3M_004974"
     CDS             2108..3163
                     /locus_tag="ACHY3M_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319700.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3164..3529
                     /locus_tag="ACHY3M_004975"
     CDS             3164..3529
                     /locus_tag="ACHY3M_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319701.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3526..4215
                     /locus_tag="ACHY3M_004976"
     CDS             3526..4215
                     /locus_tag="ACHY3M_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4319702.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4411..4486
                     /locus_tag="ACHY3M_004977"
     tRNA            4411..4486
                     /locus_tag="ACHY3M_004977"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4444..4446,aa:Ile,seq:cat)
     gene            4577..4650
                     /locus_tag="ACHY3M_004978"
     tRNA            4577..4650
                     /locus_tag="ACHY3M_004978"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4611..4613,aa:Arg,seq:tct)
BASE COUNT         1248 a         1055 c         1244 g         1124 t
ORIGIN      
        1 tttcatgata accaataaaa ggagtttttt atgaggaaca taatggcagg ttttttaata
       61 ttcctgtctt ctgctgctta tgctgatatc aatctgtatg gtcctggtgg cccgcataca
      121 gccttgcttg atgcagccaa actttatgcc gaaaaaacag gtattatagt gaacgttcat
      181 tacggcccac agaacaaatg gaatgaagat gccaaaaaaa atgcagatat cttgtttggc
      241 gcatcagaac aatctgctct ggctatcatt cgggaccata aagacagctt cagtgaaaaa
      301 gatattcagc ctctttatct gcgaaaaagt attttactgg taaagaaagg taatcctaaa
      361 aatatccgga gtattgacga cctgaccaga cctgggattg gcgtaattgt taatgatggt
      421 ggtggtacca gtaatacatc aggcactggc gtctgggaag atattgccgg acgtaaaggg
      481 aatatagaaa ctgtcgccgc aatccgaaaa aatattattt tatatgcgcc caatagcgga
      541 actgcacgta aggctcttga gaatcagcct ggagcagatg tctggataac ctgggctgac
      601 tgggcagcca gtaatccaga aattggtgat gtcgtggaaa tagcgccaga ctacgtgata
      661 tggcgtgata tgaacattac agtacgtcag gatgcaaatg atgaaacccg tcgatttgca
      721 gaatggctac aaaccgatga agcggcgcct gcattcaaaa aatatggctg gaccaggaaa
      781 ggcacttgac atcctcgtcc ttcaggacgt ggattctttt tccggatgcc gcgccagcgg
      841 catgtagggg cagctcacaa aacggaaaaa attgtacgct aagcctcgcc aggtgaactg
      901 aattcattcc gatatgggaa ttcccatatc gggcgaaaac ggtttgctgt aacggcagag
      961 ttaagtagga ttgctgcggg tgcttgaggc tatctgcctc gggcatgaac accaacggca
     1021 gatagataaa agccccaccc gactataaat cgaagtgagg cccctatatg ctcgtcacat
     1081 atagattgcc tcttacggac cgaaaggtca aggagaagca ggctatgaag cagcaaaagg
     1141 cgatgttaat cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga
     1201 ggaaagacct ctgcgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcacag
     1261 cttacgaacc tgaggagtaa gagaccaggc gggggagaaa tccctcgcca cctctgatgt
     1321 gtcaggcatc ctcaacgcac ccgcacttaa cccgcttcgg cgggtttttg tttttatttt
     1381 caacgcgttt gaagttccgg acggcgccgg aatagaatca aaaatactta agtagcgcgc
     1441 agggagaaga gggatggacc ccgaacaggg gagtgctatt tatctggaag gattctgttg
     1501 atgagaatcg aagaattacg tgaaattttt agtgaagatg gcctctatac tgtgcgcgtt
     1561 gagaagggcg ctattgtcag ccactgccgt attaaatgtt tacagtctca acaaaggaag
     1621 agtggagctg cgttaattca ttttgtggat gggcttgtga cggatggttt tattttgcgt
     1681 gcaaatgaat ttgtcacatc gttgccgtct ctgaaagaag ctgggattaa ggctggtttt
     1741 tctgcttttg aagattagtg aattcatcta caattcagcg cagggctgaa cccctgttga
     1801 gtaacactgt gccaccggag aaagccgatg gcgcaaaatt ccagactaca caattctgat
     1861 aattcagccg tctttgccag caggcacggg cggcgttctc atgcattcaa atctgactgg
     1921 ttccggcacg ccccatgcac tgaagaacag gccgaatggc tgattcagaa ctaccgcaga
     1981 cgtggggatg agtttaggaa agccctcagc ctcgattatc gtcactggat aatctacgtc
     2041 aggctgccgt actccgagcg cccaccgcgt ccgtcccgca cattccagca acgcatctgg
     2101 aggtaacgtg cgggtattac ttcgacctgt tccggtaccg gaacttgggt tggtggtcct
     2161 taaaccaggc cgtgaatcca tgcaggtatt ccataaccct cgagtgcttg tggagccgga
     2221 accgaaaagc atgcgcggtc tgccgtccgg catcgtccct gccgttcgcc agccgctggc
     2281 ggaggataaa tcattactgc cgtttttcag caatgagcgt gtgattcgtg ctgttggtgg
     2341 cgctggtgca ctgtctgact ggctgttgcg tcatgtcaaa tcctgccagt ggcctcatgg
     2401 tgactatcat cacagtgaaa ccgttattca ccgttatggt accggcgcaa tggtgttgtg
     2461 ctggcactgc gacaaccagc tgcgtgacca gacatccgaa tcactcgagc aacttgctca
     2521 tcaaaacctg tcagcatgga tgattgacgt catcggtcac gcaataagcg gtacgcagga
     2581 gcgtgaatta tctctggctg aattatcctg gtgggcggtc cgcaatcagg tggcggacgc
     2641 gctaccggaa gcggtattac gtcgttcgct ggggttgcgt gcggaaaaaa tccgctcaat
     2701 gtaccgtgaa agcgacatcg taccgggaga gcagaccgcc accatcatac tgaagcagcg
     2761 cacaaaaaat cttgcgccgc tgcctcacgc ccaccagcaa aacccgccac aggaaaagac
     2821 ggtggtcagc attgccgttg atccggagtc accggctcag tatctccagc gccagaaacc
     2881 acaacgggaa gagatgcctg tatacacgcg ctgggtaaaa acgcagaaat gcatgacgtg
     2941 cggtaatcag gcagatgatc cgcatcacat cattggtcat ggactgggag ggatgggaac
     3001 aaaggctgat gatttgtttg ttattccgct gtgccgtaaa tgtcataacg aactgcacgc
     3061 tggggtaaaa gattttgaag aaaaacacgg cagccagctg ttgttgctga ttcgtttttt
     3121 aatgcacgcg agaaattcgg gtgtcctgaa gtggaaagca tgaatgactg aacggataga
     3181 atttgttttg ccttacccgc cgacggtgaa tacctactgg cgacgtcatg gcaatacgta
     3241 tttcatctcg gaagccggaa agcgttatcg ccgtgatgtg gcgctaattg ttcgccagca
     3301 gcggctgaaa ttaaacctgt ccggaaggct ggtgataaag attattgcag agccaccgga
     3361 taagcgccgt cgtgacctgg acaatatcct gaaagcacca ctggatgtgc tgacgcatgc
     3421 cggacttctc atagacgacg agcagtttga tgaaatcaat attgtgcgcg gtcagctcgt
     3481 tcctggtggg cggctgggga taaaaatcac agaactggag tgcgcatgaa taaccactat
     3541 ttacagtttg tgcgtgagca gctcattatc gccaccgctg atttgagtgg ggcaacaaaa
     3601 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
     3661 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
     3721 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
     3781 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
     3841 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
     3901 tggaatgaat ttaatactca atccggtacc agaaaaattg ctgggaaaac gcaggaacgc
     3961 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
     4021 ggttatgaat atcaggagct ggcattactg gcgggagtga caactaaaaa ctggtccaaa
     4081 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
     4141 ttattatttg taatgagaac acgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
     4201 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
     4261 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt ttgttgtgcc acttatctcg
     4321 gatagacatg gtgaatgcgc tggtggagga gataagggtg atttttgtat gcttgcaaca
     4381 ttgatttcgt aacgttatta tcctgcgccc ggccctttag ctcagtggtg agagcgagcg
     4441 actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccaaccg ccactagcca
     4501 tcaggaaaga gcgtcaaccc tttaagttga gtgtgcgagg ttcgagtccc cggtggtggt
     4561 ccattatcgg tattctgcgt tgttagctca gcaggacaga gcaattgcct tctaagcaat
     4621 cggtcactgg ttcgactcca gtacaacgcg ctacacttat tttcccggct c
//
LOCUS       DAZUFB010000211         4651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZUFB010000211 DAZUFB010000000
VERSION     DAZUFB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.212"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..772)
                     /locus_tag="ACHY3M_004979"
     CDS             complement(<1..772)
                     /locus_tag="ACHY3M_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFS4319703.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVL"
     gene            1187..1942
                     /gene="actS"
                     /locus_tag="ACHY3M_004980"
     CDS             1187..1942
                     /gene="actS"
                     /locus_tag="ACHY3M_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFS4319704.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2021..2094
                     /locus_tag="ACHY3M_004981"
     tRNA            2021..2094
                     /locus_tag="ACHY3M_004981"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2053..2055,aa:Gly,seq:ccc)
     gene            2328..3473
                     /locus_tag="ACHY3M_004982"
     CDS             2328..3473
                     /locus_tag="ACHY3M_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFS4319705.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKELSRF"
     gene            3758..3943
                     /locus_tag="ACHY3M_004983"
     CDS             3758..3943
                     /locus_tag="ACHY3M_004983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319706.1"
                     /translation="MIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASM
                     TLMLKEMSNLINDEGIK"
     gene            3956..4075
                     /locus_tag="ACHY3M_004984"
                     /pseudo
     CDS             3956..4075
                     /locus_tag="ACHY3M_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            4035..4651
                     /locus_tag="ACHY3M_004985"
                     /pseudo
     CDS             4035..4651
                     /locus_tag="ACHY3M_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         1402 a          955 c         1079 g         1215 t
ORIGIN      
        1 ccagtacatc ctgtttatta ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc
       61 atgaccaggg aatatccagc gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg
      121 tgctggaaac gatggtaatt cgcgagtcat cctccatcca cgccagcgat gttacgcttt
      181 ccatgtgaca atgttgaata acgggggtct gatagtgccc ctgtacctgg tagtcggcgg
      241 catcgattgt ttgttggaca ttacccgtcg acatcgtgct ttgtttcagt aaattgccac
      301 cgttatggat tggtgcagcg tcttctgcca gcgccgcttc tggcgtggtg ataacgggta
      361 attcttgcca ctcaatgctg accaattgcg ccgctttttc tgccgtgagt tcatcgcggg
      421 ccacgacgat ggcaacggcg tcaccatgat gacgaacatg gcgagttagc agtgcgcgat
      481 cggcggtatc gcgcttgttt tcgtcaagtg tccaggcatg cccagcagta gcaaatggaa
      541 tatcaggcac atcttcccag gtaaaaatcg ccagtacgcc cggcaaactt ctggcttgtt
      601 catcattaat acttacggca taaccatgtg cgatagggct acgtacatat ttcgcataac
      661 acatgcccgc cataacataa tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg
      721 catcgacgcg catgcatgat tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc
      781 cctcgatttg aacatttggt tcttatggca aagatttagt ctaagcaggc aagatttacg
      841 ccagaaacga cgataagcgt taggggaggt aggaagtgtg atgacgttaa aaaaacacag
      901 atatgatacg aaacaggact ctgaataggg gcagtgatac gtatcaatat gagtggcgat
      961 aaaatcaatg tgataaattg ggattattac gcatcaggat gggaaatagg gtaaaaagtc
     1021 aggaagttcg atacctctac agcgaatatt ttgctgatga tacttccctt tgccaaatga
     1081 aattaacctc tttctctatt accacgtttt tccagaagca agagattggg tatcaagagg
     1141 ctggctgcta tgataaggcg tcttgttttt taagcgagga aagattttga gtgcgggacg
     1201 cctgaataaa aaatctctgg gtatcgtgat gttgttatcg gttggactgc ttttggcggg
     1261 ctgttcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa
     1321 acggggggat acgctatatc gtatttcgcg caccacggga accagcgtaa aagaactggc
     1381 gcgactgaac ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg
     1441 tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc
     1501 atcggttaca ccgtcatcag cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg
     1561 ttggttatgg ccaacgacag ggaaagttat catgccgtat tcgacagcag atggcggcaa
     1621 taaagggatt gatatctcag ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa
     1681 ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga ttaaacacag
     1741 tgaagattac attacggctt acgcccataa tgacacgatg ctggtaaata atgggcaaag
     1801 cgtgaaggct gggcaaaaaa tcgccactat ggggagcacg gatgcggcat ctgttcgcct
     1861 gcatttccag attcgttacc gtgcaacggc aattgatccg ctacgttact tgccgccgca
     1921 gggcagcaag ccaaaatgct gatggcgaat taatcagcag tcagcagcgc ggcacttgct
     1981 aaggagagcg taaggtttat aatgccttac gcatctcgaa gcgggcgtag ttcaatggta
     2041 gaacgagagc ttcccaagct ctatacgagg gttcgattcc cttcgcccgc tccaatttat
     2101 cttcacctga tcaatctgcc atttttgctg tatctgattt tctatattat cttaattcaa
     2161 tcgcttcaga cttttcgcaa tcgccgtaat acttccccat tttgttgtaa ataaaaagcg
     2221 tttacgcaat gcaatccttt tgtaacaacc caggtaaaac accttatttt caagttgtta
     2281 aataagtggt tatctacgct tttaacaaac gattgatgga atattttatg aaagggaaat
     2341 ctgcattaac tcttctgctc gctggtatat tttcgtgcgg cacatgccag gcgacagggg
     2401 cagaagtgac gagtgaatct gtatttaaca ttcttaattc cacgggggca gcaacagata
     2461 aaagttattt atcgttaaat ccagataagt atcctaatta tcgtcttttg attcactcag
     2521 ccaaattaca aaatgaaata aagagccact ataccaaaga cgaaattcaa ggattgctaa
     2581 cattaacaga aaacacacga aagttaacgt tgacggagaa accctgggga acgtttattc
     2641 ttgcttctac tttcgaagat gacaaaacag cggcagaaac ccactatgat gctgtctggc
     2701 tgagggatag tttatggggc tatatggcat tagtatcaga tcagggaaat agtgtagccg
     2761 caaaaaaggt tctgctcacc ctgtgggatt atatgtctac gcccgaccag attaagcgga
     2821 tgcaggacgt cattagcaac cccaaacgac tggatggagt tcccgggcaa atgaatgttg
     2881 tgcatatccg atttgacagc aactcccccg tgatggcaga tgtgcaggaa gaaggcaagc
     2941 cgcagctgtg gaaccataaa caaaatgatg cgttaggtct ttatcttgat ctccttatac
     3001 aagcaatcga cactgataca attaacgctg aagattggca aaaaggtgac cgattaaaat
     3061 cggttgcgct gctgattgct tatctggata aagccaattt ctatgttatg gaagattccg
     3121 gagcatggga agaagatgcc cgattaaaca catcttccgt tgcactggtg acctctggac
     3181 tggaacgttt gtcgaactta ctctcgaaga aagactctgt ttttgtctct gatttactac
     3241 gcgaagctaa agccaatgaa ctggatgaac ctctttcaac aacgaggttg aaccacctca
     3301 tagataaagg ttatgaaaga ataacgttac agcttgattt agggggagaa tccccaggtt
     3361 atctggaaaa agataaacat tacagagaag ccgatgcggc tttacttaat gttatttatc
     3421 ctgcaaatct tgcaaagata aacaccagaa gaaaagagct aagcaggttc taaaaatagt
     3481 taaaaaacta gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc
     3541 aaatttttgg ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaagaga
     3601 aaagagcttc attccatcaa cagaagcaga atggtttttt gactcatggt atgcaaaatc
     3661 agcagcaatt ctttataaag aatcaagaaa ggaagagtat ttgaatgact cagtgcaatt
     3721 tatgaataga tcattggccc aaataacagg cgagaatatg attggtgcaa atggcaggag
     3781 tgtacctgaa atggcacttc ccgaaagcta taactatatc cacaagtcgg gaactttgca
     3841 tgaagcgcca agccctatca ttccgctaaa ttggtcgaaa gcttcaatga ctctcatgtt
     3901 gaaagagatg tccaacctta tcaatgatga gggaattaaa tagttttcat aatgagttac
     3961 agcacaacgt tgggtattga ctgagcaaga tgaacgacaa ggacaacgac atcagcctta
     4021 tgaggagcaa gaaaatgaaa gcaaattcga aaacgatcag aaggatgaaa gttaatgttc
     4081 ggtctgagga aagtaaatcg aaacactcat acgtttgaaa ccacttttca aaattggaaa
     4141 gaaaatggcg aagatgttgc attattaatg cccgaattta gtgctaaatg gttaccgata
     4201 gcagaagaga gtggcagctg gtctggatgg gtattgcttg gagaaatatt tcccttaatt
     4261 tcatctgagc tcgctgggat ggctttaatg ccagaaacgg aaaggttaat aggggaatgg
     4321 ctcagtttat ccagttcccc gttaaatcta aagcatcctg aactaaaata taatcgtttg
     4381 cacgtaggta aagtatttga tggagtattg agccctgctc aaccactaat aagaatatga
     4441 acaggggaat tgaatctttg gttagataaa gtcacagtct gccaatacgg aaacgctcca
     4501 gtgttagaca aaaaatcatt atattggtcc attcattttg taattggatt tagtaaaaca
     4561 tgttacagaa gtcttgttga tattgaagtt ggtgatgttt tattaatctc aaataatttg
     4621 gcttatgcgg ttatttataa tacaaaaatt t
//
LOCUS       DAZUFB010000212         4638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZUFB010000212 DAZUFB010000000
VERSION     DAZUFB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.213"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..69)
                     /locus_tag="ACHY3M_004986"
     CDS             complement(<1..69)
                     /locus_tag="ACHY3M_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319707.1"
                     /translation="MQQLSFLPGEMTTRERSLILRAL"
     gene            complement(85..570)
                     /locus_tag="ACHY3M_004987"
     CDS             complement(85..570)
                     /locus_tag="ACHY3M_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4319708.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLISQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
     gene            complement(662..1480)
                     /locus_tag="ACHY3M_004988"
     CDS             complement(662..1480)
                     /locus_tag="ACHY3M_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS4319709.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     SYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGLSGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(1570..2400)
                     /locus_tag="ACHY3M_004989"
     CDS             complement(1570..2400)
                     /locus_tag="ACHY3M_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319710.1"
                     /translation="MRCDPDAGDNENGYGSCPFLGSIYDADPVFKKDLDDALKSAGLT
                     GLTGKQGSMNGPDSGLIPVVVGDEKWLLGSVCERGNCGDHYLKILYIPSEHVIAGFYY
                     NSGEENMFGDVGDAEARLLRRDVPDVTPVQQAPALPVTHTDGAPADTIWTFKGDSSKT
                     LWQVCGGQSSSCAIIANTKYYVAVLNRKSATGCAFGDFYVAARDTATWRQYDTGTCSA
                     DAYIRKGSINNGQYLSVDIGINGVLVKQFPIGYWSMQKEFSGNRRPSWSKVKEKNQQH
                     "
     gene            complement(2574..2984)
                     /locus_tag="ACHY3M_004990"
     CDS             complement(2574..2984)
                     /locus_tag="ACHY3M_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319711.1"
                     /translation="MKAFILSLIVSGLLLSASVNATEVKLPRTVIDVKDGSEQMCGSE
                     DIKDGICSLIGVSDDGKYALLSSPGGSACYDPYIIVVNLATSKGKMMDYNRHATCSGE
                     ATAKFVRSSDKDHLTVVIYDPSIKKNVGLNTIDF"
     gene            complement(3101..3934)
                     /locus_tag="ACHY3M_004991"
     CDS             complement(3101..3934)
                     /locus_tag="ACHY3M_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFS4319712.1"
                     /translation="MNIEKSRLISEAAPHLNASLSTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGVAYRK
                     IKQSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYLRQ
                     LKARISAANNFKPMCDALNMARAEQGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVVH
                     V"
     gene            complement(3947..4378)
                     /locus_tag="ACHY3M_004992"
     CDS             complement(3947..4378)
                     /locus_tag="ACHY3M_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319713.1"
                     /translation="MNKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHINHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            complement(4378..4575)
                     /locus_tag="ACHY3M_004993"
     CDS             complement(4378..4575)
                     /locus_tag="ACHY3M_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4319714.1"
                     /translation="MSQSFIRLSEVQRRTGYSKAWIYRLIKEQRFPQSVKIGSRSIAF
                     IESEIEEWINQRIAESRGEVA"
BASE COUNT         1121 a         1161 c         1134 g         1222 t
ORIGIN      
        1 cagggcccgc agaatgaggc tgcgctctct ggttgtcatt tctccgggca gaaaggaaag
       61 ctgctgcatt gttccggccc cctttcagtc gatgatgcgc ataatggcgt tgcattccgg
      121 atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat aacactccgt
      181 acgacaggca tgatggctgt acgtcatcag acaggcggca ataccggcgg cttccgggct
      241 catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttctc catcatcttc
      301 atccggttct ggagccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc
      361 accacagtag tcttcgcaaa gacggtccat ccagccgaag atacggggct ccagggtcac
      421 ccactgtgaa atgaggccaa aatgttgcgg ccagaagctg atgcgctgtt catcagggac
      481 gagagtagca accagctgag gtgggttatt ccctgatgca gcggttacgg aaacagaagg
      541 tgtggtggaa ttatgcgtaa cggttgtcat gagattattc cttataaaaa gtaaatgaat
      601 ggaagaatgc ccgggggaag ggacagccgt gagtcaggac tgcgctttca gggaaaaggc
      661 atcagcgcat gctctccagc agcgtttctg ccatcaccca caatgcccgg ttgagcttaa
      721 tgtcggtatc gatgctgtga atggcacggg tgtggatacg ttttcctttt gcactgcgac
      781 cggaaagccc gcctttcagc atattctcct ggatggtctg ataagcactc cacaggtcct
      841 taccgtaatc ctccctgcgt cgtggtgtca gaatgtcggc ggtggtgacg ggctgatgtt
      901 cgtcaccata acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca
      961 ggaccagcga ctgcatggca tcacgcttct cctctatccg gtcaaacaca cccaccacct
     1021 cgtaagcccc ttcaatgact ctgtccacca catttccccg gtgcggaaca cgcacttccc
     1081 ccagagactg accacagacg cagccgttct ggcagacgaa tctgaagtaa cccggcagca
     1141 tctggtagct ggaggtaccg tcatgggagt tgagcagaat aatttcaggg acatgttgtc
     1201 cgtttatctc cccgtcacgg cgcagacgca gcatgtgttt ggtatacccc cggcggcccg
     1261 ggtcacgtac gcgggtctgg caggcgaaga atggctgaaa gccttcccgc tgcaggcttt
     1321 ccagcactgt gatggtgggg atgtacgaat accgttcact gcgggaggta tgccggtctt
     1381 ccccaaaaat gctgggaaca tagtgcatca gttcttcatg ggtcagtggg cggtcacggc
     1441 gtatctgatt aatccgtccg aagcgactgg ctaatcgcat aattaactcc ttatctgata
     1501 atgaaataaa agcaaaaagg ccatgtcccc ggaggaacat ggcctgatgg aatatattat
     1561 tgctgatgat taatgttgct gatttttctc tttaactttt gaccatgagg ggcgtcggtt
     1621 gccggaaaat tccttctgca ttgaccagta accaatcggg aactgcttca ccagcacccc
     1681 gttgatacca atatcgacgg acagatactg accgttgttg atgctgccct tgcggatgta
     1741 cgcatccgca ctgcaggtgc cggtatcata ctgccgccat gttgcggtat cgcgggcggc
     1801 aacataaaaa tcaccgaaag cacagcccgt tgctgactta cgattcagaa cggcaacgta
     1861 atacttagtg ttggctataa tggcgcaact gctgctctga ccgccgcaga cctgccataa
     1921 cgttttgctg ctgtcaccct taaaggtcca gatagtgtct gccggtgcgc cgtctgtatg
     1981 tgttactggc aatgccggag cctgctgcac tggtgtaacg tcgggtacgt cgcggcgtaa
     2041 tagtcgtgct tcagcatccc cgacatcgcc aaacatgttt tcctctccgc tgttgtaata
     2101 aaaaccggca ataacatgtt ctgaggggat atacagtatt ttcagataat ggtcaccgca
     2161 attaccccgc tcacagacag accccagcag ccacttctcg tcaccaacga ccaccggaat
     2221 taatccactg tcagggccgt tcatggagcc ctgttttccc gttaacccgg tgagtcctgc
     2281 tgacttcagc gcatcatcaa ggtctttctt gaataccggg tcggcatcgt agatactgcc
     2341 cagaaagggg caggaaccgt atccgttttc attatcacca gcatcaggat cacagcgcat
     2401 tgcatctgct gccgtttttg cgacaccggc agcgtaaact ccggtggaaa taaaacaaag
     2461 cgaggatatc agtattatct gttttaattt acgtaataca agaaacatga ttatttttac
     2521 tccatcctgt ttatataaaa gaaacgggct ccgtgaagag ccctgtaaaa aatttaaaag
     2581 tcaatggtat taagaccaac attctttttg atggagggat cgtaaataac aacggtaaga
     2641 tggtctttgt ctgaagaacg gacaaactta gcggtggcct caccactgca tgttgcatga
     2701 cggttataat ccatcatttt acctttgctg gtagcaagat tgacaacgat gatataagga
     2761 tcataacagg ccgaacctcc cggactgctc agcaaggcat atttgccatc atcagagaca
     2821 ccaatgaggc tgcatatccc gtcttttata tcctcacttc cgcacatctg ctcacttcca
     2881 tctttcacat caatcacagt acggggtaat ttaacctctg tagcattaac tgatgcactc
     2941 aacaacaaac ctgaaactat taatgacaat ataaacgctt tcatatactc atcctgttta
     3001 tcattacggg ggatatattc cttttattct gctttcgaca gtaacgagtg ctccgtgggc
     3061 atccttgtag cgtttttgag gttgtggtgt tcctgtcggc ttaaacatgg accacctcca
     3121 gacgcttagc gagccagcgt tgcgacaggc gggtgagttc tcctttgcgc tgctcatatt
     3181 ccatgcccat atcaattaac gtgatattgg tgctttcgag atatgagagg tgctcaagtt
     3241 gttctgcgtt catatggtcg cgaggttcgc cagaatagcc atttatccgc gcccactgct
     3301 tagcagttag cccaccaaga acgatacgag aaatcatatt gctctcgttt gtgtagtggt
     3361 gttgctgcgt cgttttcccc tgctcggcac gggccatatt cagcgcatcg cacattggct
     3421 taaagttgtt tgcggcactg atacgggctt ttagctggcg aagatagcgg gcggcgattt
     3481 caggaacgct acgctgtaat tcttcctcgc actggatgaa ataacggcgg atagcgcgac
     3541 cctgatcatt gcgttcgatc attgccagtt ctttcgccat tccgacactt aacagatagt
     3601 ctttgccggg tctgccagac tgcttaatct ttctgtaagc cacgccattc gggcttttcc
     3661 ccaaatttgg ggaaatagtt ttatggactg agtaatcgtg cccaatggtg aagtcatatt
     3721 cagagatgcg atcagtgatc catgtagaga agtcttttcc cacgcccaac gctttgtgta
     3781 acgcttttgc gctaacaata ttggtttcac gcccaccgat atgaccagga ataaccggga
     3841 caattgcggc aaattcatta ccgttaattg tgctcagaga ggcgttcaga tgaggggcgg
     3901 cctcagaaat taatctgctt ttttcgatgt tcattttcag tctccgttaa gcggcgttaa
     3961 acgcgtccgg gtagagtttc agaatgtcgg caatttcctg ctgagaaagt ccgttgtgat
     4021 catttatgtt cgcaagatgg ttaatatgag tgatcacttt cagaacatca ccacggcagg
     4081 agaaacggta gcgaaggtgt gttccgatac catcaggatt cttttcgtcg atacgctcca
     4141 gagtgattcc aagctggcgt tctaactctg ttgcatagtt acgaccagaa gacagacggc
     4201 agtagcgaag aatgtcattt tctgtccatc cctctatacc agtacgcagc atatagactc
     4261 tggcacgatg ctttttcggt gtgcgctttg gtgcttgaag ggattttgta aggggcgtaa
     4321 catcagatac gcgaatatct gaattagccg cctgtaatgg gcggtttttc ttattcatta
     4381 cgctacctcg ccacgcgact cagcgatgcg ctgattaatc cattcttcaa tttcactttc
     4441 aataaacgca attgatcggc ttccaatctt tacagattga gggaatcgct gttctttgat
     4501 gagccgataa atccacgctt tgctgtaacc agtacggcgt tgaacctcag aaagacgaat
     4561 aaatgattga gacatgttta ctcctatatt ctgtcatctt ctttaatgga catcagtaga
     4621 cagaaaggag attaaaca
//
LOCUS       DAZUFB010000213         4526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZUFB010000213 DAZUFB010000000
VERSION     DAZUFB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.214"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..537)
                     /locus_tag="ACHY3M_004994"
     CDS             complement(<1..537)
                     /locus_tag="ACHY3M_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS4319715.1"
                     /translation="MKQQVSFKFRLKPDGQQECQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTETEWLIDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEISTPVHPSASM"
     gene            637..3624
                     /locus_tag="ACHY3M_004995"
     CDS             637..3624
                     /locus_tag="ACHY3M_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319716.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTTVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMSVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEVRAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDKNASALWHELGHHLEYSNPGLLEKAQSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNSGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            3614..3934
                     /locus_tag="ACHY3M_004996"
     CDS             3614..3934
                     /locus_tag="ACHY3M_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319717.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDEDDDDDEEVFSDETVN
                     QLLY"
     gene            complement(4138..4425)
                     /locus_tag="ACHY3M_004997"
     CDS             complement(4138..4425)
                     /locus_tag="ACHY3M_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319718.1"
                     /translation="MEKLNNFLDKISTPNTRAGHTITRIAKLSDAGVSDDVIALQLSS
                     NSANGFRYTADEIKAYKKLHNDVKTKVGITSKQTKALIKDQELTFVALPQN"
BASE COUNT         1188 a          973 c         1168 g         1197 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagtgga tatttcactt tctgtctgaa tactgatgta
       61 ccacttaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 attccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc gaaatgcatc attctgtccc cgctttttga atcggggaaa
      241 agcagcccga ttctggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggagaat ctataagcca ttcggtttca gtgtcttttt tccactcaac
      361 caaccaggaa gccattttcg tgtaagggat gtatttttta ccggcctcat gattctcatt
      421 ctgaagcgcc agagcacggt tgaaaacgaa acgacaagct ccggcaaagc gcctcatttg
      481 acactcctgt tgaccatctg gttttaaccg gaatttgaat gacacctgtt gtttcatgtc
      541 actattttaa gcaaaaatag ccgggaggaa aactgtgcct tatatcccca ctatgaggag
      601 cgtagggggc ggcacattgg ataaggcgta acagggatga gcttaagcga tcaggtggta
      661 atggccacca gcatagaaac gctgatcgag ctgctaaaga acctgcccga ttatgggcgg
      721 gtttcgtatg tggtgacagc gaagggagac gaggtaaaaa cagcgtttga tatcgtcgat
      781 gcctcagctc ttttggtatc caatactctg gatgggaaaa ttaatccaga ctatccccag
      841 gaacttcagc cgcgcgaccg gacccgcgca tccagccttc ttcaggtcaa ccagatatcc
      901 aaggatttgc gtcctgccca gcttaccgat tccggtttat ccagccatgg cgcgccaata
      961 attggtgagg acaatgccgt tgagtcaggt aatgggcgga ccatggggat cattaaagcc
     1021 tatcaggacg gtaatgcgga tcggtatcgg gagtacctga ttgaacatgc gaccgaattc
     1081 ggcatacggc ctgaaaaggt tgaatcaatg acggctccgg tactggtgcg ccgccggtta
     1141 acgacggttg accgtgttca gtttgccaag gactcaaata tttctgatct ccaggaaatg
     1201 gcagccagtg aaaaggcttt tgttgatgcc gacagcataa caccggcgat gatggcgctt
     1261 tttaacccgt cagaaagcgg agatctgctt agccgcagta atgacgcgtt tattcgcgga
     1321 tttatgacgc aagttggtgc cacacaggcg gctggccttg taactgaaga tgggcgacca
     1381 acacggcaac ttgttgaccg tatacaaaac gcgatctttg ccaaggcata taaggatgcg
     1441 cgcctggtaa ggatggttgc agaagaacct gatccggata tgcgtaatgt tctgacggcg
     1501 cttaatgcgg cagccaatga ttttgtccag atgcaggctt tatcaggaga agcgcacaag
     1561 caggctgtga caactattgt tgatggcatt gagacagcgg atagcctcga taaaaaggcg
     1621 ctggcggcat tgaaagatgc ggtagacctg gtaaggcaat cgaaggagtc aggccagcat
     1681 attaccgatg ttattgctca gggggatatg ttcagcgaaa cggccccgga ggtgaaagca
     1741 ctcgcgttgt tcatcgtcgc gaataaccgt agcgcgaagc gtatggccac cgcctttaag
     1801 ttgatggcgc aacgtatcaa tgatgagtta cagcaccagg gccaggcgct cggggatatg
     1861 tttggcggcg gcgatgtgtc gttacaggat atccttcgcc aagtatctca ggaactggaa
     1921 aacgaaggta tgcaagggat atctggcggt cttttcgagt cagcttctgg cggtagttac
     1981 aacggtgttg ctccatatac cagcttgcta ttacatcggg catccggcat caaagacatt
     2041 attcatctga tcaggctgct ttcccgtaca gatccccagg atgaacagct tgttcaagtg
     2101 cttgcgcatt ttgttcgaat gtctgttgcc gacgtgaaaa aatggtgccg attattcggt
     2161 atcagcaatt cgttacttcg cggcttgtta aatcacgcat cttcccttgg gcgcgatggc
     2221 tttgacgaga tagcgcaggc gataaaaaac ggagatatgc caccagctat tgactggttt
     2281 tccattcgcc caaccagggt gaaagcattc cttagcgcgg cgcattcggc atcaccattg
     2341 gcagaaatgg ttcagaggtt atcgctcata ttcacagacc ataccgcgtt gggtgatctg
     2401 actctggacg agatgaagga agcctccatt cagtgggccg atcaacaaaa tgaggttaac
     2461 tcagacttct tgccagcatt caggaaggcc gttagtaaag cggatgatgc ccgtggaatt
     2521 ctgaaggcat ttaaggcatt gcaaagtcgt gttaataaac atgtcggtga tatcgatggg
     2581 gtaacggcgg aaggccggga tatccttaaa gagcacggca taacgccaga gtttattgat
     2641 gagatcagga ctgatatgca gcgtgaggtc gtatcgtccc tgcaaatcgt agccagagcg
     2701 ttggcggatg ctaatccgaa gagtgcggcc attgttaacc gggttattgg tgatattgaa
     2761 gcatcggagg gcatgggggc gctgaaactc ttcctttcgc gagcgtttaa tcctaacggc
     2821 aatattctcc ctggcattat tggtgaggct aaaaagtatg tcagtgaaga agaacttgag
     2881 cagcttgacc aactacttaa gcgattctca tataacccgc agacacgctg gcaaatgaat
     2941 cagcgaagta tgggttcggt ccacgagaaa gtgttatctg ccatgaacag tgcgatcgca
     3001 aactcatccg tatctgaaga aaaagctctt gagtgggccg actcttttat cacagaagaa
     3061 gtggaagaag tccgcgctgg acagaatggt gggatagacc tgcgcaagga acttgctgat
     3121 atttatcgcc tgaccggcgg gaaaatatcg accttatcaa aggtagttca ccaccaggga
     3181 agggcatatg caaatctaaa tggtgttgtt gctgtcaatt tgaacgataa aaatgcaagt
     3241 gcactgtggc acgagctggg tcatcatctt gagtacagta accctggttt gttagagaaa
     3301 gcccagtcat tcctgaaggc caatgttgaa ggggataagc catcattcgt caatatcggt
     3361 gggcgtggca agcctgaatg gtgcttcaga tctcgattga gtaatattta tatggcgaag
     3421 gtatacccgc cagcctcagt aagtaattcc gggaaaattc ggcagaaatc accgactatt
     3481 tccaaaacgt cagcaacgga agtattctct atggctcttc agttgtatca tgacaaagag
     3541 gccgctgccg catcactgat gaatggtgac ggattgctgg aactgttatt aggtgtggca
     3601 aaggagctaa ataatgcaga ttaaaatcgc agcgccatta ggcggagatg ccattatcga
     3661 atttgatgat aatgaagaag tttccgggcg tttgagcatt atctccggtg acattaccga
     3721 ggacatgatc gctgaagcca tagctggggc aaatcccaat agctatatgg gattcgttaa
     3781 cacccttgat gctcccgcaa gtgatgttct ccgaacgctg catctttacg ctggctggtt
     3841 tgttgattgg ccagcagtag atggtggcga tgaggatgat gatgacgatg aagaggtttt
     3901 tagtgatgaa acagtaaacc aactcttgta ttgacaggaa ttggcataga attgattgtt
     3961 caaatttcat ccatcacata gacgacttca tgttatttgc ctgataatta tggccttaat
     4021 aacccatgta tatcttttgg gttatattct tatctttaac aaggttataa gagcgacaat
     4081 gataaattta tcactatcgc tcttataact ctttgttagc tatagcttta aacaaattta
     4141 gttttgaggc aatgcaacaa aagtcagctc ttgatctttg attaatgctt ttgtttgttt
     4201 ggatgtaatt cccactttag tttttacatc attgtggagc ttcttatacg cttttatttc
     4261 atctgctgta tatcggaagc catttgcact gttactggac agttgaagtg cgattacgtc
     4321 atcactgaca cccgcatcgc ttagcttagc tatgcgggta atagtgtggc ctgcgcgagt
     4381 gtttggggtg gatattttgt ctaagaaatt gttcagtttt tccattgttt acaacctctt
     4441 acttgagttt attgccagta gaattgccac tattcttttg cctcaacgaa tcacaatgta
     4501 ctggaataag aaactatgag caatac
//
LOCUS       DAZUFB010000214         4485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZUFB010000214 DAZUFB010000000
VERSION     DAZUFB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.215"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            208..1167
                     /locus_tag="ACHY3M_004998"
     CDS             208..1167
                     /locus_tag="ACHY3M_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFS4319719.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(1311..1382)
                     /locus_tag="ACHY3M_004999"
                     /pseudo
     CDS             complement(1311..1382)
                     /locus_tag="ACHY3M_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(1379..1567)
                     /locus_tag="ACHY3M_005000"
     CDS             complement(1379..1567)
                     /locus_tag="ACHY3M_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFS4319720.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1564..1926)
                     /locus_tag="ACHY3M_005001"
     CDS             complement(1564..1926)
                     /locus_tag="ACHY3M_005001"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319721.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRIIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(1923..2213)
                     /locus_tag="ACHY3M_005002"
     CDS             complement(1923..2213)
                     /locus_tag="ACHY3M_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS4319722.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSDCHAEIDRRTRILDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(2206..2418)
                     /locus_tag="ACHY3M_005003"
     CDS             complement(2206..2418)
                     /locus_tag="ACHY3M_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319723.1"
                     /translation="MSDYLKWYLCHRWLIKFAVKDWMTADANKLKQRKDYYYARMKEN
                     YCSIRTRIFIKKDLQSILQLRGKVNG"
     gene            complement(2411..2587)
                     /locus_tag="ACHY3M_005004"
     CDS             complement(2411..2587)
                     /locus_tag="ACHY3M_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFS4319724.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELM
                     SDPNSSMYEEEDDE"
     gene            complement(2587..2946)
                     /locus_tag="ACHY3M_005005"
     CDS             complement(2587..2946)
                     /locus_tag="ACHY3M_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HFS4319725.1"
                     /translation="MDYSQLSDFEINVAVFEAIHNGSPDYKEGENGDMVFVSFEGDIV
                     NGDAVEVEVERGSFNPCANPADSWPIIEKYRISIINLDEDEWGARGVAYCKSKRAIHE
                     NPLRAAMIVFLMMQRIQ"
     gene            complement(2949..3125)
                     /locus_tag="ACHY3M_005006"
     CDS             complement(2949..3125)
                     /locus_tag="ACHY3M_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS4319726.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(3122..3532)
                     /locus_tag="ACHY3M_005007"
     CDS             complement(3122..3532)
                     /locus_tag="ACHY3M_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFS4319727.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVFPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGHGVRFTDRRYE"
     gene            complement(3504..3866)
                     /locus_tag="ACHY3M_005008"
     CDS             complement(3504..3866)
                     /locus_tag="ACHY3M_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319728.1"
                     /translation="MDESRKQFEESWLRRGGESSDLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIELDIDWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIR
                     AAGIKEKNFDEANNLPPN"
     gene            complement(3884..4090)
                     /locus_tag="ACHY3M_005009"
     CDS             complement(3884..4090)
                     /locus_tag="ACHY3M_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319729.1"
                     /translation="MAELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SIFLWVIIGVMPVAVAKMAWSFVS"
     gene            complement(4163..4453)
                     /locus_tag="ACHY3M_005010"
     CDS             complement(4163..4453)
                     /locus_tag="ACHY3M_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319730.1"
                     /translation="MTGKEIILEYLKTHEQFSPHELALITGIPNNRIAQAARHMVKQG
                     HLSVVERKWKTVIYAKRKVKKEPIKRNPDGTGWGCANPMTAFINRALMEVRQ"
BASE COUNT         1192 a         1072 c          903 g         1318 t
ORIGIN      
        1 ataaaaatta taaaaaacat aaaacaccac gcaaacacac ttaataaaaa caccgttaca
       61 ttaaaagata ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata
      121 aaacatcata ttgattacgc accttgtatt acaaactcat gtatgtaaaa tacgcgcacc
      181 attcaaaaaa aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt
      241 gctctgtcat ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat
      301 gatgatgcag ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac
      361 agagatgatt actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc
      421 caattatctc tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac
      481 aacacagctg taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg
      541 aactcccttg cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc
      601 cccgacgcag gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa
      661 tatcatgcat gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta
      721 tccatgacac caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct
      781 gctgttactg gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat
      841 gataaaacct tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat
      901 atgtccgata caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc
      961 aatcgcaact atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta
     1021 aacaaaactg ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc
     1081 acactagcat ttaataaagt aacatcagaa ttcaagacag gggatgacta ttcatttata
     1141 aatggagcaa tcaccttctt tttttaactg aattgaattc aatcaaaata acataagtcc
     1201 aacaaaaaca taaagtgcga aatgaatgcc agctccattt atttcgcact ataaaagatt
     1261 aaaagttgca ataaaataat aaaatgactc agttacgaaa accaataaac tgtggccagt
     1321 agtgagtcgc tcatcatcgg gctttttggc gaatgaaatt tagctacgct ttcgagtctc
     1381 atcgtcttcc cctcttgccc tgtttgacca tcaggacgcc gttaactatt acatgacgct
     1441 cgcctttgct gtctcggttg tacttgagca ctgttcctct tgcgcaggaa agcatcctcg
     1501 ccacttcggt ctgattgcct cgtgtctgga taagaagctc tggtatcgtt tgaattgtgg
     1561 cgttcatgcg ttctccagtt cggtgatttt tattccaagc cgtccgcctg gtactttcac
     1621 accacgaatt atgcgaatgt catcgaattg ctcgtcgtct tccgcaaatc cggcatggat
     1681 aagggagtcg agtaaaccct tcaggatgtt atctaggtcg cggcggcggg agtctggaac
     1741 gtctgcgatt actttgatgc ggagtcgtga tttggtgaaa atatctaact tgagttggtg
     1801 gatgatttgc tgaacgtctt ttcggtattt ctggccttta tcgctgatgt agtattggct
     1861 tccccgtctt cgccagtagg tattcaccga cggcgggtat ggaagcacaa actgatattc
     1921 gttcatgact taatcttccc ctccttcagc agtatcgcct gcgtcctgat cacgccttcg
     1981 aggtggtaaa gtctggcgtc tttgttgtcg agaatccttg tgcgtcggtc gatctccgcg
     2041 tggcagtcac tacaagccca tgcgccgatc aggtcgtcag gcttcatccc cgttccacaa
     2101 attccagcca tccggtaatg tgccagaact gtagtttcag gattgccatt gcatacgccg
     2161 taaatacgta cctggcattc tctgccgcgc gcttctttgc gtaggttagc catttacctt
     2221 ccctcgcaat tgaagaattg actgaaggtc ttttttaata aatatgcgag tgcgaattga
     2281 gcagtagttt tccttcattc tggcgtagta atagtccttt ctttgcttaa gtttgttggc
     2341 atccgctgtc atccagtctt ttacagcaaa cttaattagc cagcggtggc agagatacca
     2401 tttcaggtaa tcactcatcg tcttcttcct cgtacattga gctattcgga tcgctcatca
     2461 gttctgcaca gcagtgctca cacacgtgaa cttccagcac atgcagcttc tgaccgcagt
     2521 tagcgcacgt taaagctcgc tcgacgcttt ctttctggta ttgaagggat tgggatgggc
     2581 taagcattat tggattctct gcatcatgag aaagacaatc atggcggcgc gaaggggatt
     2641 ttcatgtata gctcgcttag atttacagta ggccacaccg cgtgcacccc actcgtcttc
     2701 atcgagattg ataatgctaa tcctgtattt ttcaataatc ggccatgagt ctgctgggtt
     2761 tgcgcatggg ttaaaggatc cgcgctcaac ttctacttca actgcgtctc cgtttacaat
     2821 gtctccctca aatgagacaa acaccatatc gccattctca ccttctttgt aatccggcga
     2881 tccgttatga atggcttcga ataccgccac gttaatttca aaatcactta actgtgaata
     2941 atccattgtc atttcctcgc acgatgtctt agccaccgga tatcccacag gtgagccgtg
     3001 tagttgaagg tttttacgtc agattctttt gggattggct tgcgtttatt tctggagcgt
     3061 ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg tgatacttcg tcgctgtcgt
     3121 ctcattcgta cctcctgtcg gtaaatctga caccatgacc aatagcccag gctgttgtgt
     3181 actcgataag acttgccata cgcttcacac tcatctgcgc gctgctttcg cgaatgttga
     3241 cgtattcgcc ttcaagcccg ggaaaaacat cagcttcctg ttttgttgcc actgcatgac
     3301 cgctgatcaa caaaaccttc cattgttctg gttttaacca tttgccgcac cattgaacct
     3361 gacgagcgat atccgccagc atcgcgtgaa attttgcgtt ctggtcaagg ttgcgcttgt
     3421 agtcagtaat gcggatggtg actggcttgt ctttatcgag tggtgttgcg aggatggcat
     3481 ttattgcagc ttgctgttgt tgcttagttc ggaggaagat tgtttgcttc atcgaaattc
     3541 ttctctttaa ttccagcggc tctgatagct ttcattactg caattaccgt tttgtctctc
     3601 ccatcctcat aacccatcgc ataagcacct tcttcaccat ctttccaaaa gtcgtcattc
     3661 gattcgggcc agtcaatatc cagttcaata gctgctcgcg atgcctgcca cgtttgccag
     3721 tggccttgaa catcgtccat cacgtattga ccaccaatat caccactgcc aatttcatgg
     3781 tgattttcag ggtaacggat aaggtctgag gattcgcctc cacgtcgcaa ccaactttct
     3841 tcaaactgct ttcttgattc gtccatcgat acttaccctc agttcaactc acaaaactcc
     3901 acgccatttt tgctacagcg acaggcataa caccgataat cacccacagg aaaatgctac
     3961 cgaaaagcac acccaccagg tctttacctt cgcctaccaa ccggacaaaa ctgctggcaa
     4021 caacaatgaa cgtcgccacc atccacatag caccgagaat cctcaatgca gagaaaatta
     4081 actcagccac gatttaccct cccccaaata aaaaggcctg cgattaccag caggcctgtt
     4141 attagctcag tgatgtagat ggtcattgcc ttacctccat aagcgcccta ttaataaacg
     4201 ccgtcattgg atttgcacat ccccaccccg taccatctgg atttctttta attggctcct
     4261 tcttcacttt gcgttttgca taaataaccg tcttccactt acgctcaaca acactcaaat
     4321 gtccttgttt caccatatgc cttgctgctt gagcgattct gttatttggt attccggtga
     4381 tcagtgctaa ttcatgtggg gagaattgtt catgagtttt cagatattcc aggatgattt
     4441 cttttccagt cacgatctgc tcctgtaact atcccatgta aacgc
//
LOCUS       DAZUFB010000215         4424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZUFB010000215 DAZUFB010000000
VERSION     DAZUFB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.216"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..813
                     /gene="nanC"
                     /locus_tag="ACHY3M_005011"
     CDS             97..813
                     /gene="nanC"
                     /locus_tag="ACHY3M_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFS4319731.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            833..1939
                     /gene="nanM"
                     /locus_tag="ACHY3M_005012"
     CDS             833..1939
                     /gene="nanM"
                     /locus_tag="ACHY3M_005012"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFS4319732.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGVSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2004..2984
                     /gene="nanS"
                     /locus_tag="ACHY3M_005013"
     CDS             2004..2984
                     /gene="nanS"
                     /locus_tag="ACHY3M_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS4319733.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(2992..3096)
                     /locus_tag="ACHY3M_005014"
                     /pseudo
     CDS             complement(2992..3096)
                     /locus_tag="ACHY3M_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3196..4068
                     /locus_tag="ACHY3M_005015"
     CDS             3196..4068
                     /locus_tag="ACHY3M_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFS4319734.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVEKLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            complement(4179..>4424)
                     /locus_tag="ACHY3M_005016"
     CDS             complement(4179..>4424)
                     /locus_tag="ACHY3M_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319735.1"
                     /translation="NPASYNDLINGLSEDFKKRTVMLVDQIPEKQKSRYHINEDALIT
                     LPSGEIVAISNQWGIVNIELLIEFVRQNGFVVEKAEQ"
BASE COUNT         1305 a          946 c         1026 g         1147 t
ORIGIN      
        1 actataagcc tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt
       61 gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct
      121 ggcgtattat tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta
      181 cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga
      241 tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat
      301 aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc gattaaactt
      361 gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc
      421 tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc
      481 tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat
      541 aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca
      601 tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca taagaaatgg
      661 gcgacggaaa atgcatttgt tctacaatac catatgacgc ccgatattac gccatacata
      721 gaatatgact acctggaccg tcagggtgtt tacaacggca gagataattt atcggaaaac
      781 agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa
      841 aacaataacg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc
      901 tgaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat
      961 tggtttaggt agcgcaggta cggcatggta caagctggat acacaggcca aagataaaaa
     1021 atggacagcg ttagctgcat tccctggtgg accaagagat caagcaacct cggcatttat
     1081 tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaat agcaagggct tgactcaggt
     1141 atttaatgac gtacacaaat acaaccccaa aaccaatagt tgggttaaat tgatgtcgca
     1201 tgcaccgatg ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac
     1261 tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa
     1321 agattcagca actatagaca agatcaatgc ccattatttt gacaaaaaag cagaagatta
     1381 tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg
     1441 cgaatctccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg
     1501 gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt
     1561 caccggtaat aatttaaaat ggaataagct tgctcccgtc tcatcaccag atggcgtcgc
     1621 tggcggtttt gcgggggtaa gcaatgattc tcttatattt gccggagggg ccggattcaa
     1681 aggttcacga gaaaattacc agaacggaaa gaactatgcg catgaaggcc tgaaaaaatc
     1741 atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc
     1801 gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtctattga ttattggcgg
     1861 tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atctctgtga aggataataa
     1921 agtcacagta caaaattaac gcttcagggc cccggtaaag gggccctgct atcaatttgt
     1981 catttcaatt acgcgaaatt tatatgaacg caataatatc gcccgattat tactatgttc
     2041 ttaccgttgc tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca
     2101 gggaagatgc gcctcatccc agaattaaac aattagcgag atttgcgcat acgcatcccg
     2161 gaggcccgtc atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc
     2221 aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg
     2281 ttggccaggc actgcatatt gcacggaaat tactaccctt tattcctgat aatgcagggg
     2341 ttctcatcgt tccatgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat
     2401 attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctgt
     2461 accaggattt agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc
     2521 tcggcgtatg ctggatgcaa ggagaatttg acttaatgac cagtgactac gcgtcacacc
     2581 ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc
     2641 agcttaataa tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag
     2701 aaaatttccc gcattcgtat gaagctattt atggcaatta tcaaaataat gttttagcca
     2761 atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg
     2821 aagatccgga cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact
     2881 ggacgacggc actgcgaagc agtcatttca gcacggcagc ccgtcggggg gttatttctg
     2941 acaggtttgt agaagcaatt ttgcagtttt ggcgcgaaaa gtgagcgttc attaattgga
     3001 tttattcgca tccatcaacg cttttaactc ctccggcgta aacggtggtt tgcgccggtg
     3061 cagcatcgca tggcaattag ggtacacagg cagcaaaaaa acaaacgaga tcaatgtaat
     3121 atatcttcac ttgcaggctt gaacagcaga aaatttgcga gcatgatcgc aattatttca
     3181 ctgccaggga tatcaatggc ttccagtaaa tcgctacagc aggcaattgc caatataaaa
     3241 atctggcata agggtgaaca gcgcgcaccg cataaaccat tgttattgct atacgtatta
     3301 gcgggatacc tgaatggaca tccacgcctt ttcgattatg gatcggaaat ctacgaacct
     3361 ctgcacagtt tactggaacg ttttgggcca cagcgttcac agtaccgacc tgatatgccg
     3421 ttctggcgat tacaaggcga cggattctgg caattacaca atgccgagct ttgctcaaca
     3481 gcagggagca gccgacaacc gccagtgaag gaactgaacg aataccacgt tgcaggcggt
     3541 ttcgatgaac agcactacgc cctggtaacc ggcaataaaa aactcatcaa tactctggcg
     3601 caacagatac tcgaagcgca cttcaccgag agtattcagg aagaaattgc tgacgagctg
     3661 ggttttgatc tccagcaaat ccgtaaacag cgcgacccgt tattccgcaa aaatgtatta
     3721 cgcgcttata actaccagtg cgcgatatgt ggctttaata tgcgccacga cgacaccacc
     3781 gtcgcgttgg aagcggcgca tataaaatgg aaacagcaag gaggtccttg tgaaattcct
     3841 aatggcctgg cgctttgcgc aattcaccat aaagcctttg ataaaggatc gattggcctg
     3901 gatgaaaata tgcgggtact ggtatcagat gcggtaaacg gcggagggat cgttgagaaa
     3961 ctattctggg attttgacgg gaaaactatt gcgttaccac aggtgcgtaa aaattaccct
     4021 tttgaggggt ttgtagagtg gcatcggaaa gaggttttta gggggtgagg ttgataatac
     4081 cgcgccccca taatcttgcc atctcgtgca ttgagatgtt aaaagctaca aatgttctcc
     4141 ctatttttta atgtagacat ttatagcaat atcaaaattt attgttcagc tttttcaaca
     4201 acaaaaccat tttgccgtac aaactcaatc aacagttcaa tattaacaat gccccattga
     4261 ttagaaatag caacaatttc accagaaggg agtgtaatca acgcatcttc attgatatga
     4321 tagcgactct tttgcttctc tggtatctga tcgacaagca taactgtacg ttttttaaag
     4381 tcttcactta atccatttat taaatcatta taactagcag gatt
//
LOCUS       DAZUFB010000216         4331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZUFB010000216 DAZUFB010000000
VERSION     DAZUFB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.217"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..867
                     /locus_tag="ACHY3M_005017"
                     /pseudo
     CDS             1..867
                     /locus_tag="ACHY3M_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1386..>4331
                     /gene="efa1"
                     /locus_tag="ACHY3M_005018"
     CDS             1386..>4331
                     /gene="efa1"
                     /locus_tag="ACHY3M_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFS4319736.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAITDIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFFLENMIKLQDNFFNYCIV
                     KGVTEINDELRINYLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFGENRISI
                     KDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSK
                     QVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIKKI
                     LTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDA
                     VIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFR
                     QDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGT
                     GKYDWLTPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSTLKTENLPELFLTKIDT
                     DTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDI
                     AKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHKKNDNDLSMSIKGFLASNPHTKIN
                     ILYSNKTEHN"
BASE COUNT         1540 a          788 c          834 g         1169 t
ORIGIN      
        1 tgcgcccgtt cctccgtcag gcgctggatt aactggttca cgctgtcgag tgttgaaggt
       61 ctgaaatcat tacccgcagg acgttcccgc cgctggccgt ttgagcatat ctgcacattg
      121 tttcgtgaac tgataaaaca ctctcccgga tattttggct atcagcgttc acgctggagt
      181 acagaactgc tggcaataaa aatcaacgag ataaccgagt gccagttaca tgccggaacc
      241 gttcgccgct ggttgccatt agtcgggctt gtgtggcgca gggctgcgcc aaccctgcgt
      301 acccgtaacc tgcataaagc agcaattcac aaagcactgg acgaatgtag tgcggagcat
      361 ccggtctttt atgaagatga agtgtatatc tatcttaatc ccaaaaccgg tgcggactgg
      421 caacttcgca gacagcaaaa acatgtggta acaccggggc agaatgaaaa atactatctg
      481 gctggtgctt tacatagtgg aacgggcaaa gtcagctatg tgggtggcaa cagcaaaagc
      541 tcagccttat ttatcagtct gttgaaacat ctgaaattga cataccgacg ggataaacct
      601 atcacgctca tagtggacaa ctacatcatc cataaaagtc gggaaacaca gcgctggctg
      661 aaagaaaatc cgaagttcag agtgatttat cagccagttt actcgccgtg ggtgaatcat
      721 gttgagcggt tatagcaggc gcttcacgat acgataacgc gcaatcatca gtgccgttca
      781 atgtggtaac tgcttaaaaa tgttcattat tttatggaaa ccgtgagccc attccccggt
      841 ggtaaatatg ggctattaaa agtgtagcgg tattcggcgc agctatttag gatggtcagg
      901 tcgcagaagt tctgtgccac tacagtgcgt gtactaaaca aaatgacgag ggcacgaatg
      961 tcataatacg tgcgtattac ctgacagtag ctaccaactg agggggctta ttccaaacct
     1021 gatttattca acaaagctgg acttatcctt actctgaatt attttccaga catatgcatc
     1081 atgtactggc tcgcttggtt caggaaatac aggagcaaat ctacagctat tagatttatt
     1141 cctatttgta tgcaaagtcg cattgcataa tgcggagcta tgttacagat acgcctgacg
     1201 tgcgtagtaa aattataaag caacatatat atacaaggag aaaccgagaa aaataaatga
     1261 gtcgtatgtt gggtagcatc gagataaata tttttttatc acattagcac cttggttaag
     1321 ggtgttaata ataatattct ctcacttatt aaaacataaa taatttttta agcggaaatg
     1381 taattatgag actgccagag aaagttcttt ttcctcctgt cactagtggc ctgtcagggc
     1441 aggaaaaaca aaaaaaaccg aagagcatta ccggatttca ggaaaattat caacgcaata
     1501 tcaggccaat caaaacagca tcagaagccc gactacgctt ctttgataaa atggtttcga
     1561 aagaaaactc tctggaagat gttgtttctt taggtgaaat gattcagaag gaaatttatg
     1621 ggcatgaaca aagaacattt tcaccagttc atcatacagg taactggaaa tcatcattgt
     1681 tacacaacgc gctccttggt ctggcaaatg tttataatgg cttacgggaa acagaatacc
     1741 ctaacacttt caacagagat ggtataaaaa gtactaactc ttttagagat aacttattga
     1801 caaaaacaag aactcccaga gataattttg aggaaggaat aaaacatcct gaacatgcaa
     1861 caataccata tgacaacgac aatgaaagta ataaattgct aaaagcagga aagatagctg
     1921 gtaacaataa cgagctgttg atggaaataa aaaaggaatc ccaaagcgac catcaaatcc
     1981 ccctgtcaga taagttcctg aaaaggaaaa aacgatctcc tgtagctgaa gataaagttc
     2041 aaaactcgtt aacaccagaa aattttgttc agaaaatttc acttagtgat gagcttaaaa
     2101 caaaatatgc aaatgaaatt atagagataa aaagaataat gggagaatac aatcttttac
     2161 cggataaaaa cagtcgtaat ggtctaaaac ttctacagaa gcaagctgat ttactaaaaa
     2221 taatcatgga ggatacctct gttacagaaa atacctttaa aaacatagag atggctataa
     2281 cagatataaa aagggagtac tattcgcata cagttgatat tgagaaaaat attcatgcca
     2341 tatgggttgc gggttcccca cctgaaagca tttcggacta tattaagact tttctcaaaa
     2401 cctacaaaga gtttacttac tatctttggg ttgatgaaaa agcatttgga gccgcgaaat
     2461 ttaccagtgt cttaaaacaa attgcatttg atttagcatg cagaactata cagcagaata
     2521 ctccacaaaa aaatattgat tttattaatc tatataatga aataaggaag aaatacaaca
     2581 acaacccatc gggacaacaa gagtacttaa acaaactcag agagctttat gctacttatc
     2641 aaaaaatatc cacccctctg aaacacatgt ttaattcatt ttttttagaa aacatgatta
     2701 aacttcagga taatttcttc aactattgca ttgtcaaagg tgttacagag attaatgatg
     2761 agttacgaat aaactacctt aaaaatgtaa taaaactgtc agacgatgac attggtaatt
     2821 atcagaaaac aattaacgac aataaagata gagtaaaaaa actaattctt gatttacaga
     2881 aacaatttgg tgaaaaccgc atttcaatta aagatgttaa ctctttaacc tctctttcaa
     2941 aatcagaaaa taatcacaat tatcaaactg aaatgttgct tcgatggaac tatcctgccg
     3001 cctcagacct gctcaggatg tatatcctta aagagcatgg tggtatttat acagacacag
     3061 atatgatgcc tgcatactct aaacaagtaa tttttaaaat tatgatgcag acaaacggag
     3121 ataatcgttt cttggaagat ttgaaactac gtcgtgcaat atctgatggt gtattaagat
     3181 atgttaataa ccaaaatatt gatgaagtta actataatga aatcagtgat gcagataaaa
     3241 acattatcaa gaagatatta acagaaatat ctaaaatgcc agaagatagt atttttacta
     3301 agatcaatac aaggattcct cgagacacaa tgcccatcct tcgtcgttat cacctatggc
     3361 ctgatggatg gaatattcgt gggctcaatg gattcatgct atcacataaa ggtagtgaag
     3421 tgattgatgc tgttatcgca ggccagaatc aggcttacag ggaactaaga agaataagag
     3481 ataatattca tagtgaaata tacttcaaac aaactgatga attgtcctca cttccagata
     3541 cagacaaaat tggagggatt ctggtaaaaa aatacctttc aggaagtctt ttttcaaaat
     3601 tcagacaaga tactattatc ccggaggcat tgagcaccct ccagatatca ggtcctgact
     3661 tgattcaaag aaagatgttg caatttttca ggagtagagg ggtgttaggt gaagagttca
     3721 ttaatgaaag aaaactgagt gataaagctt atattggtgt ctacaaaaca actggcacag
     3781 ggaaatatga ctggttaacc cctgaatcga tcggcgttaa tgatgtcacg cccgcagatg
     3841 aaagtacctg gtgtatagga aaaggccggt gtgttgatga cttcctgttc aaagatgttt
     3901 caacactaaa aacagaaaat cttccagaat tattcttaac aaaaatagat actgatacgt
     3961 ttttctctca gtggtcaacc aaaaccaaga aagatctgca aaaaaaaata caagacctta
     4021 ctgtacgtta taatgagcta attgactcgt cgactatcga ctttaaaaat ctatatgaaa
     4081 tagatcaaat gctccatatg attatgctag agatgaatga tgatatagcc aaaaggtcat
     4141 tgttttcatt gcaagttcaa atagccgaaa aaattcggag gatgaccatt cctgtagaca
     4201 atataattaa catctatcct gatctacata aaaaaaatga caatgatctg agtatgtcca
     4261 taaaaggctt tcttgcgagt aatccacata caaaaataaa tattctttat agcaataaga
     4321 ctgagcataa t
//
LOCUS       DAZUFB010000217         4323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZUFB010000217 DAZUFB010000000
VERSION     DAZUFB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.218"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(267..1916)
                     /gene="pgi"
                     /locus_tag="ACHY3M_005019"
     CDS             complement(267..1916)
                     /gene="pgi"
                     /locus_tag="ACHY3M_005019"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4319737.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2153..2349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2441..3790
                     /gene="lysC"
                     /locus_tag="ACHY3M_005020"
     CDS             2441..3790
                     /gene="lysC"
                     /locus_tag="ACHY3M_005020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFS4319738.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3935..>4323
                     /locus_tag="ACHY3M_005021"
     CDS             3935..>4323
                     /locus_tag="ACHY3M_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4319739.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAK"
BASE COUNT         1037 a         1079 c         1099 g         1108 t
ORIGIN      
        1 acgattaaaa atctatttta ggattaacct catggtatga tttccgttaa atcacagaca
       61 aggggattta tctgataaaa aaatgccgac ataacgtcgg cattgttatt aaggttattt
      121 aatcaatcgt gctatgtatt aattgccgaa tacgatgtac atatcggcat cgacctgtag
      181 gcctgataag acgcgaccgc gtcgcatcag gcatcggttg ccggatgcgg cgtgaacgcc
      241 ttatccggcc tacatatcga cgatgattaa ccgcgccacg ctttatagcg gttaatcaga
      301 ccattggtcg aactatcgtg gctgctgatt tctttgccat ctttcagctc tggcagaata
      361 cggttcgcca gctgtttacc cagctccacg ccccactggt cgaaggtgaa gatgttcaga
      421 atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc caggctgaac
      481 ggagtgattt cacgcagcag gatggagttg gtcgggcggt taccttcgaa cactttgaac
      541 ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc ctgctcaacc
      601 acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt agacagcagt
      661 ttctggtgat gatcagagag cgggttatgg gtgatagccg gagcgatgaa atcgcacggt
      721 accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt agtgcctggt
      781 tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg gtcaacatac
      841 ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg catatactgg
      901 tcatacggca gaatcgcctc agtttccgca ccaaagaaat tgttgtacca gatgccaatc
      961 agcgccagca gtacaggcag gtttttctcg gcaggcgtgg tggagaaatg cttgtccatc
     1021 gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag aacaatcgac
     1081 aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa ctcgaacatg
     1141 ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag cgccgcaaag
     1201 tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg cgcgctatgg
     1261 gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa cagcgtggtt
     1321 tccgggttta cttttttcag cacttcagcg atgtgagtcc catcgacgtt agaaacaaag
     1381 tgcatgttca ggtgattttt gtacggacgc agtgcttcgg tcaccatgta cgggccgagg
     1441 tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt ataacccttc
     1501 cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac cgcgttgact
     1561 tccggcatta cgtctttgcc atcaaccaaa atcggggtat tgctacggtt acgcagcgct
     1621 acgtgcagca cagcgcggtt ttcagtgcgg ttgatcttct cgccagagaa catcgactta
     1681 atcgcgcccg ccagatcgca ctctttcgcc agatcttgta atttcgccag cgtctcttca
     1741 gtgatgcggt ttttggagta atccaccagc atctgatcgc cgaaggttgc ggagaactta
     1801 gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt catttcatcg
     1861 aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt tttcattagc
     1921 aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag tcacagaaaa
     1981 atgtgatggt tttagtgccg ttagcgtaat gttgagtgta aacccttagc gcagtgaagc
     2041 atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg accccgtcgt
     2101 tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag cgcaggccag
     2161 aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata acacctgagg
     2221 gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta caggggctga
     2281 atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg gtggagcact
     2341 tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt
     2401 gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc
     2461 caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg ctgatattgt
     2521 gctttctgat gccaacgtgc gtttagttgt cctctcggct tctgctggta tcactaatct
     2581 gctggtcgct ttagctgaag gactggaacc tggcgagcga ttcgaaaaac tcgacgctat
     2641 ccgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga
     2701 gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc tggcaacgtc
     2761 tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt
     2821 tgagatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta aagtgatgcg
     2881 taccaacgac cgatttggtc gtgcagagcc agatatagcc gcgctggcgg aactggccgc
     2941 gctgcagctg ctcccacgtc tcaatgaagg cttagtgatc acccagggat ttatcggtag
     3001 cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata cggcagcctt
     3061 gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc cgggcatcta
     3121 caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga
     3181 agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc
     3241 agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg caggtggtac
     3301 gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa
     3361 tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct tcctcgcgga
     3421 agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca cgtcagaagt
     3481 gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata cgttgctgac
     3541 gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaagtagaag aaggtctggc
     3601 gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca aagaggtatt
     3661 cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca gccataacct
     3721 gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg cagaagctgc attttaattt
     3781 gtttgagtaa tatttcccta ctggcgtata acgctcaccg attgttatac gccatcctca
     3841 ctgaaaacat agattaaata atatctgaaa agcaaaatca ctcctgtaga acagtattat
     3901 aatcaccgaa aaatatcatt taacaggagt gttaatggct actttaatgg aaaaagatgc
     3961 acttttaaac ggagccagtc agtgcatcgc gtttttatca aatatcattg ataattgctc
     4021 agtttcgtcg catcaggatt caggcgatgc tctcaagcga ttagtcagct atcgcgatta
     4081 tctttattct acccccgctg aacttgttga ttttacacaa ggtaagattc tccttcagca
     4141 ggtaagaact caatatcagc atgaattcaa caatactacg cattcagaaa ataaagcatc
     4201 ttttgattct atttggcagc gtctgacaaa tcatgaagtc acaccgcaac agcatcccat
     4261 tggttttgtt ttaggtgggc aacccggagc aggtaaatca tctctgattg aattagccaa
     4321 aag
//
LOCUS       DAZUFB010000218         4289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZUFB010000218 DAZUFB010000000
VERSION     DAZUFB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.219"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            430..1443
                     /gene="nleC"
                     /locus_tag="ACHY3M_005022"
     CDS             430..1443
                     /gene="nleC"
                     /locus_tag="ACHY3M_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFS4319740.1"
                     /translation="MYKTILSINMKIPVLQPSFNFFAPAGYSAAVAPNRADNAYADYV
                     LDIGKQTPLSAADLSNVYENVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNKEVYIGSIKYRNEYERNEESPVKVDDIQSLTCTELYEYDVGKEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVTNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1564..1647)
                     /locus_tag="ACHY3M_005023"
                     /pseudo
     CDS             complement(1564..1647)
                     /locus_tag="ACHY3M_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            1732..1806
                     /locus_tag="ACHY3M_005024"
                     /pseudo
     CDS             1732..1806
                     /locus_tag="ACHY3M_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(2678..3208)
                     /gene="yodB"
                     /locus_tag="ACHY3M_005025"
     CDS             complement(2678..3208)
                     /gene="yodB"
                     /locus_tag="ACHY3M_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS4319741.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(3551..4201)
                     /gene="zinT"
                     /locus_tag="ACHY3M_005026"
     CDS             complement(3551..4201)
                     /gene="zinT"
                     /locus_tag="ACHY3M_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFS4319742.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1229 a          833 c          948 g         1279 t
ORIGIN      
        1 gaaaatattg gcggaatgaa atagccgaca gcttcacaaa aaccggagtc cggctccggt
       61 tttttgtgtt gcaatgtccg ggggatattt gttaagtaaa tgcattttca tgcactgcgg
      121 ttttagctga tatattaaca agccatgcaa agggaaattt tactgatatt tttgagcagt
      181 attatccatt agcatggcgt tgatgtctgt tgctacgtga tgccaatata tattgcaaat
      241 cacagagcag gcagctcatt aaacttggta agccttgcta cgataaggaa ggtttaccat
      301 tgtggtgtca ttagctcaca tgtgtatggg agctttaaac gttcctgtta ctcgttggaa
      361 cacctgcctt gcagggataa aagctatgct gtccgttatc gtctgggcta gtgaattggt
      421 ggcactgaaa tatataaaac catattaagt atcaatatga aaattcccgt tctccagcct
      481 agtttcaact tttttgcccc tgctggatac tctgctgccg ttgccccaaa tcgtgcggac
      541 aatgcctatg cggattacgt tttggatata ggcaagcaaa caccactttc cgcggcagat
      601 ttaagcaacg tatacgaaaa tgtcattcgc gccgtccatg acagccgtag caggctcatc
      661 gatcagcata cggtcgatat gattggcaac actgtacttg atgctttgag tcgatcacag
      721 acctttcgtg atgccgtaag ctatggcatt cataataagg aagtatacat tggtagcatt
      781 aaatacagaa acgaatacga acgtaacgaa gaatcccctg tcaaagttga tgatattcaa
      841 tcattaacct gtaccgaatt atatgaatac gatgtcggga aagaaccaat tctccccatt
      901 tgcgaggcag gagaaaacga gaacgaagag ccttatgtca gttttagtgt tgcgccagat
      961 actgattctt atgagatgcc atcgtggcag gaaggactga ttcacgagat tattcatcat
     1021 gttactgggg ccagcgatcc atctggagat agtaatatag agctaggacc caccgagatt
     1081 ctcgcacgtc gtgtcgctca agagttggga tggagtgttc ccgacttcaa aggatatgca
     1141 gagccagaac gagaagctca tcttagacta cgtaacctga atgcccttcg acaggctgcc
     1201 atgaggcatg aaaagaatga gagggctttc ttcgaaagac tgggtacgat cagtgaccga
     1261 tatgaggcga gtcctgattt cacagagtat tccgctgtga ctaacatagg atacggattt
     1321 atccagcaac atgattttcc aggggtggct atcgacgaga atttacagga tgtaaatcag
     1381 atccaactgt atcatggtgc tccttatatc tttacatttg gggatgtgga cagacacaat
     1441 tagcgctgat tcgtctttac agttacatag ggttactact catagatatt aacggagttg
     1501 atgatgggca atcgtgcaac attgtattgt atgtaaaggc agagccccga attttactgt
     1561 tgtaagtggg cctggcggga atggtatcgc cagcatcatt ggcttgggtc cagtaaaaag
     1621 agacgcagaa agatgcacaa taggcactac gcgtcatgca tggattaggt cgctcatcat
     1681 ttacacagta ctgctaaaaa attattaagg aaagcgcggc acttcgtatg taagaacgtg
     1741 tcgcggttgg ctggtgaact ttcgatagtg ctagtattgg atgatttcca gccgttaccg
     1801 attttatata acttcagtgg aattattcaa cacacaggca aaacggaata agctgagaaa
     1861 aaaagcgtgc tttcccaatc tggggtaaac cacgtctgaa tgacacctga aaacaggtgg
     1921 tggcctcaag aaagcttgga atattcttct ttaatgttat gtaatttatt gatttttcgt
     1981 gtacgatttt aagacattta ttccaagaaa aatttttaac tctttgattt ttcgactctg
     2041 tatcatcggt ctcgaaaacc ggagtagggg caactctacc gggggttcaa atccccctct
     2101 ctccgccact ttatcaatga cttatctccc gacttcccgc cttgcttttc ctaaacagaa
     2161 caatcgtaga atattcttga agggttagat cgtcactgtt ttctgttcga tactgtgaca
     2221 ttcagcactt gattcgctat ggatctgaca ggaagctttc gagcgaaaat gtgcagttat
     2281 tcagtcgttt tcttatcgct caccattatt cttttagaca ttcatcctat gaaactgccg
     2341 caaagttggt ggtgggaact gaagttgcgt agagaagggg tcaataattg agtcaaaaaa
     2401 ggaacgcggc cgtcagatta aagccgcggg acaaagtcca taatttccaa gagttgttga
     2461 atttcccagc ttttttgaaa gcctttgtat catactaaca agtaaaaaag gtttaaatac
     2521 caaaattaac ctgtattttt gataagtctt accattgtac gatttacaca gtttctgctc
     2581 ctccttgttg atagtggaaa gggtgcattg tgcacttgcg taaacattta tgacgcgttg
     2641 atgtctatgg ttcacatttc cttgtcaggg gatcgactta tttgcggcga ggcatcattc
     2701 gtaacagagt attatctttc tgaatgtagt gatgaaagag tgctgcgcca gcgtgagctg
     2761 cgattaaaaa gtaacctata tttgcccagg tttcgtgaat attttttatc agtgctttaa
     2821 tttcgctgtt tggggtaaca aagggagaca cattgaaacc aaggaaactc cacgattttc
     2881 cactgtaagc cattaaagca atccccagca gaggaagtgc aaggaaggtt atataaagca
     2941 tgatgtgcat tagcgaagcc gctttcattt gccaggcagg tggcggtggt acaatagaag
     3001 gatcactata acggtgtttt ataatcaggc gtgaaaacat taacacccaa acgaatatac
     3061 ccgcattgta atgtgtttct cgcatcagca gataagtact actaccttta ggaaaccagc
     3121 cacggagttc catcgcggca taggttattg caacgaaaag cagcgttatc caatgtaaat
     3181 aaatttgagt ttttgagaat cggttcattt ttgtagctcc tgtaattttt acagtcaagc
     3241 tatcccacga accttaaaaa atccttatac agatgtggtt ttaagaattt gttatttaaa
     3301 aaattgatag tgcacattgt aaccagccaa tataagcaat ggcgtgagca caggtatctt
     3361 tctctgttgg ccgtattgtg tatggaatcc gttattggtt aactcaatgc gggttagctt
     3421 attaatatac agacacgtca tagtgatgag cgtgtctgta gctagcttgc gttcagtggc
     3481 gtttgtggat ggaccagtgg cagttatgat gacttactaa agcggcatcg aggcgttatc
     3541 atgagaatac tcaatgagac atcatttcct cgaccacttc ttcgctactc aactgatatg
     3601 gataatacgt tggccagttt tccatttcat ttaataaaga ttgctgtgaa tcattaccca
     3661 taaaaatgtg aaaatgagat gattttcgtg gtgcaattat atgatcgcta aattgtatat
     3721 atttaggggc tttgctttca ggatctttac attcgaataa gtaacgaacg cctttcttgc
     3781 ctgatttata ggtgagtatt ttgtatccat cgtaatcata tttacaggat gttgtttcat
     3841 tatttctatg gaattcaaca atgccgtcct caatgccaat catctcgata tctgttgcat
     3901 aacctttgtg ataataatct ttaatttcag caaatgtttt agttttatct gcatccgctt
     3961 tcttctgaaa gacggggtca agtttgccac tttgcagtaa aggataaacg gattgccaga
     4021 ctccatccca gtcactgagc gttcggtttt gtacattggc atcatcaaaa acaccattag
     4081 cagctttttg ttcgacctct gttaagggtt tgccgtgtga gtgatgacca tgcgaaaagg
     4141 caggagcgct aacaataaag acacctaaag caacagccag tttgtgaaga cgaatcgcca
     4201 aaatagtttc ctccagagtc attgtatatg atgtgtaatg ttatattgta acatatggca
     4261 aaatgcaaca actcttagga gatgagagc
//
LOCUS       DAZUFB010000219         4277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZUFB010000219 DAZUFB010000000
VERSION     DAZUFB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.220"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..739)
                     /locus_tag="ACHY3M_005027"
     CDS             complement(<1..739)
                     /locus_tag="ACHY3M_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS4319743.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAY"
     gene            complement(739..1542)
                     /locus_tag="ACHY3M_005028"
                     /note="aph(3'')-Ib"
     CDS             complement(739..1542)
                     /locus_tag="ACHY3M_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFS4319744.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1603..2418)
                     /gene="sul2"
                     /locus_tag="ACHY3M_005029"
     CDS             complement(1603..2418)
                     /gene="sul2"
                     /locus_tag="ACHY3M_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFS4319745.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(2726..3577)
                     /gene="repC"
                     /locus_tag="ACHY3M_005030"
     CDS             complement(2726..3577)
                     /gene="repC"
                     /locus_tag="ACHY3M_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFS4319746.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            complement(3564..>4277)
                     /locus_tag="ACHY3M_005031"
     CDS             complement(3564..>4277)
                     /locus_tag="ACHY3M_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFS4319747.1"
                     /translation="KSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHR
                     LHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRR
                     FHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVL
                     VDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVL
                     KPAVLERQRKSKGVPRGEA"
BASE COUNT          895 a         1306 c         1250 g          826 t
ORIGIN      
        1 cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca cgagagaatg
       61 cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg tcagccggat
      121 cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg ggatctatca
      181 ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca tgtagcccac
      241 gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg acgtagtcag
      301 tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca aagcgatccc
      361 ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc ttcgccatta
      421 gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg tgagagagca
      481 ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc aaccggactg
      541 ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca gcaatgtctt
      601 ctataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa actttccaaa
      661 cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa tgagcaggaa
      721 aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat agaaggcaag
      781 gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga aggcgcgctc
      841 tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg ccaaatcggc
      901 atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt gaagagtttt
      961 agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca tatcggtgcg
     1021 ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat cgagctgcgg
     1081 cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg acaccacatc
     1141 aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat caaccgatag
     1201 gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca aatccgctcc
     1261 agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg caccctcctg
     1321 ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga gccaaatgag
     1381 gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt tcgcgaaggc
     1441 atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga caggcaacca
     1501 gtcagaatgc gattcaccaa aaaaaatatt agttcgattc aatggaggtt ccttcagttt
     1561 tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa ttcttgcggt
     1621 ttcttttagc gccgccaata ccgccagccc gtcgcgcaag gggcgcggct cgtgtgtgcg
     1681 gatgaagtca gctccacctg cggcggcggc aagctctgca gcgagtgtcg cggccccgac
     1741 atcccccgga ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa cagacagaag
     1801 caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg agagcgaggt
     1861 ttcgggagca gcccccagaa aaaaccccat gccgggatca aggacaaggc ggttgcgttt
     1921 gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa tgtgatccat
     1981 gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg aatgcataac
     2041 gacgagtttg gcagatgatt tcgccaattg cggatagaac gcagcgtctg gaaaaccgcg
     2101 aatatcattg agataggcca caccacgcga caaggcatag gcttgcgtcg cgggttgata
     2161 actgtcgagc gagacgggaa tgccatctgc cttgagcgcg tccagcaccg gcgcgatacg
     2221 ctcgatttct gtgtcggacg aaacaggcgc ggcgtcgggg ttgctggatg ccggaccgag
     2281 gtcgatcaca tctgccccct cggccatcag cttacgcgcc tgcgcaatgg ctgcgtctgg
     2341 cgccagatac cggcctccat cggagaaact gtccgaggtt atgttgacga tgccgaaaat
     2401 gatgagcgat ttattcatgg gggcttctat aataataata atcgagcatg agtctcatac
     2461 ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg atccattagc
     2521 tgcaggagca gaatagcata catctggaag caaagccagg aaagcggcct atggagctgt
     2581 gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg agcatggtat
     2641 ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag ataaaaatgt
     2701 cttgtttaca atagagtggg gggggtcagc ctgccgcctt gggccgggtg atgtcgtact
     2761 tgcccgccgc gaactcggtt accgtccagc ccagcgcgac cagctccggc aacgcctcgc
     2821 gcacccgctg gcggcgcttg cgcatggtcg aaccactggc ctctgacggc cagacatagc
     2881 cgcacaaggt atctatggaa gccttgccgg ttttgccggg gtcgatccag ccacacagcc
     2941 gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc
     3001 gcacatgctg gccgccaccc atgacggcct gcgcgatcaa ggggttcagg gccacgtaca
     3061 ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc
     3121 ggccattctg ggcgatgatg gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct
     3181 tgagcgcccc accactatcg acctctgccc cgatttcctt tgccagcgcc cgatagctac
     3241 ctttgaccac catggcatca gcggtgacgg cctcccactt gggttccagg aacagccgga
     3301 gctgccgtcc gccttcggtc ttgggttccg ggccaagcac taggccatta ggcccagcca
     3361 tggccaccag cccttgcagg atgcgcagat catcagcgcc cagcggctcc gggccgctga
     3421 actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct
     3481 cgccccgctt gagggcacgg aacaggccgg gggccagaca gtgcgccggg tcgtgccgga
     3541 cgtggctgag gctgtgcttg ttcttaggct tcaccacggg gcaccccctt gctcttgcgc
     3601 tgcctctcca gcacggcggg cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca
     3661 gcgaacggtg cgccatagtt ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg
     3721 tcgtcgtcca caccccattc ctcggcctcg gcgctggtca tgctcgacag gtaggactgc
     3781 cagcggatgt tatcgaccag taccgagctg ccccggctgg cctgctgctg gtcgcctgcg
     3841 cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca cgatagagca cccggtatcg
     3901 gcggcgatgg cctccatgcg accgatgacc tgggccatgg ggccgctggc gttttcttcc
     3961 tcgatgtgga accggcgcag cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc
     4021 ttgaggccgt cgaaccactc cggggccatg atgttgggca ggctgccgat cagcggctgg
     4081 atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg
     4141 tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg gcaggtagat caccgggccg
     4201 gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg caatctgtgc ggccagttgc
     4261 agggccagca tggattt
//
LOCUS       DAZUFB010000220         4253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZUFB010000220 DAZUFB010000000
VERSION     DAZUFB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.221"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(230..565)
                     /locus_tag="ACHY3M_005032"
                     /pseudo
     CDS             complement(230..565)
                     /locus_tag="ACHY3M_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            495..824
                     /locus_tag="ACHY3M_005033"
     CDS             495..824
                     /locus_tag="ACHY3M_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4319748.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            821..1264
                     /locus_tag="ACHY3M_005034"
     CDS             821..1264
                     /locus_tag="ACHY3M_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4319749.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            1251..1853
                     /locus_tag="ACHY3M_005035"
     CDS             1251..1853
                     /locus_tag="ACHY3M_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HFS4319750.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            1855..3774
                     /locus_tag="ACHY3M_005036"
     CDS             1855..3774
                     /locus_tag="ACHY3M_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319751.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQSGARAIGGAEIGRARNVNGLSVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLVDQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGGHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAYPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            3771..4136
                     /locus_tag="ACHY3M_005037"
     CDS             3771..4136
                     /locus_tag="ACHY3M_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HFS4319752.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGVSGGSSDEEKQPSEHDGVPKLIADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(4176..>4253)
                     /locus_tag="ACHY3M_005038"
     CDS             complement(4176..>4253)
                     /locus_tag="ACHY3M_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319753.1"
                     /translation="LACGGMVQSGRPLKQEPTEMIQATA"
BASE COUNT         1158 a          942 c         1130 g         1023 t
ORIGIN      
        1 gtcattatta atttatggct tgccatttct atacatatct gttaaaaatt ttgacttgtt
       61 tgttgaaaat agatttaatt gattggttta ttttttacat gacgctcttt aatatagctt
      121 caggtatata taagcacaat gcattatttc cagaggaaat taacaaaatt gttagctctt
      181 atttgttgag cttatagata ttaattaagg cccgattccg ggcctttcct cattccggct
      241 tttcgggaaa cgttactgga aggatggagg tatccgtgga ttcgactttc tgcgcataga
      301 gaatccactc ggttaatttt tgtttaccct gaacggaaat aatgcctagc cgtaactgtg
      361 agtcccagag ctgagtttta tccctgacga gctgtagcag gctttgcttt tctttttccg
      421 cttgttgtct ttgcatgacc ggttgagaag ttactttgca taccattacc tcctgacaac
      481 gtaggaggga acttgtgctt gacacacagg aattagctcc agttgctatt gcgctcctgc
      541 tttcagtaat tggtgggata ggcacgttcc tgatggatgt ccgagacggt cgccagtctg
      601 gcaatttgtt gggattggtt acggagatct ttgttgcagt gacagctggc gcggtggcgt
      661 acctattggg gcaacacgag ggctgggagt tatcaattac gtacttaatg gtaacgatag
      721 ccagcaataa cggtcatgag gtgatttcag ggatgaaacg agtgaatatc gatagcattc
      781 tgaatgttct tacaagtttg gtgaaaaagg gaggtgggaa atgattggct ggggtgtatg
      841 cgctcttgcg ttagccttag ccgatcgcta tttgctaaaa cgcaaggaca tcacgcattt
      901 agaacttggt gatgtggaaa ttaaaccggg tttcatccgg gtgccgttca aataccggtc
      961 taaattcccg tttttgcgtg gcgcaacggt cagatattgg atccgcgatg ttcagaagcc
     1021 aacgacagtg attgaaggcg aacaacgttg tctgacgtcg gctgaacagg gcgaaaacag
     1081 tgaatggttg tacataccca ctgaatatat gggtaaagga gaacggctgt ggcatttcaa
     1141 cgtcatggtt acgcatggcg actcgttcat taacccgttg tatcggattt tccctgttac
     1201 tcagcaaatc cgcagaagtt acgtaataaa tctcgcacag gatgtgtcag atgacgaaaa
     1261 ataagtatgc aacggtcgat tttgaccagg ttaatgaaaa ggggctgaaa tcccttatcg
     1321 cggcgatcaa taaaaccggt gttacggtaa ttgaggttga ctccagcaac cgcgcaacaa
     1381 cgaaagatgg cgttaaagtt aaaaccgcaa agctggttct taacgacgga caaattcttg
     1441 ccatacaggt aaacgatact ggcgatatat cgtctgtgag gctgaatgga aaagctattc
     1501 ctaacgctca atcgccggat atcaagacgc ttggtaccgt catggggcag gcggcccgca
     1561 aaaactccgc aaaattccag aaatcactga tcgccaaagc gaaacgtgtt gccaatccgg
     1621 tagacaagaa accggcagta aaatccaact ttcagcgcct gcaagaggca aaacagcgga
     1681 atgctcaggt ggttgccgct tataaatcag cgcagaactc ggtgtctttc aatcaacagc
     1741 agatcactga tttgcgggcg aagctggata aggagacagg ccgactcaat aacgaaaagg
     1801 cccgaaatgg cgaactcaaa cgccgtctta agcaactgaa agcaggaaat taacatggaa
     1861 cagttcaata tcaataaagg ggtgacgatc aagcctgggc ttgacgtgct tcccccgcca
     1921 gtgactgatg atgaatatcg cgcattaatg gccggtgagg accgctatct gatgacggaa
     1981 tccaacaccc tggaggaaat cgaggctacg ttcttctatg acacgccgat ccactggtgt
     2041 gctacggatt tactggaggc gattagttct actcgtttgc agttacaccg gaccatgcag
     2101 gcatttgtcc gggcattgaa ccagaagctg aatggtaccg gaatctctgc ggggagtgat
     2161 aaaacggggg atgtggccca gagcggcgcg cgcgcgatcg gcggagctga aattggtcgg
     2221 gcacgtaacg ttaacgggtt gtcggtcctg ccagccatta ttccgctcag tgatggtcag
     2281 actatcagca ttttgtttca tagcccgaca gcggaaaacc ggatcaccaa tagcgatacg
     2341 ctggttgctt tccagttctt actgaataaa aaagacgtta ctcacaccgt tgctccgatg
     2401 agtggacgtg atatgacgct ggcgcaggtc accatgaaac ttgccaacct tgcagagaaa
     2461 aactcggcaa aatttcagcg tgcgcagaag aagaaaaaag cccttgttga tgaaataacc
     2521 caactacagg ctgacagtga ccagaaagag gatgccatga gcgacctcgt ggatcaggtg
     2581 gcagcggtag aagggcagaa ggtagatctg gagcagaaaa ttaacgctgt tgcatcggaa
     2641 gcggattctc tttatgaaga gaatgagcgt ttgcagacgg agattgatca gctcaatcga
     2701 actggtgggc gcgaaaccat tgctcctgcg gggatgactg gtggacactc tcgcgcgatg
     2761 acggatcgcc ttgcaagtat caaaaatcgt atgcatatga acggggaagt gacgctcagt
     2821 aatggtgcat caatgaagca attcattgag gacggtgaag ggtatatcca gttaaccgat
     2881 tcggatggca gcgtgtacat gatcaaggct aaatccatac agggtgtgga catggcagat
     2941 gcgatcggca agctgtttaa agcctataaa gcgggtaatg tatcggaata cctggtccaa
     3001 ccagaagaac ataaaccgga aaacgtcgaa cctgaaccag cggaggatac cggtagctct
     3061 tcgcctgaac cagaagtctc tgtaggtgca tatcgatatg ccctgcaaat gcgtccggcg
     3121 gcccctggcg caatacctga aggtaacaaa gcaattctgc cgcgccctga tgaaggtgac
     3181 ccgtattatg aatatgcacg ctacggcatt gctacttacg ataccccgct ttctgatcag
     3241 caaatgagtg agtacgacct gaagttattg cctcgcgagg attctttcga cttcctggcg
     3301 aagacactta ctaatggtcc gtttggcaaa tatgcacaaa aagctctgga gctggccacc
     3361 agctcaccag acgagttccg cgtaatgctg aaaactcagt ttcaaaaaac tttccccaat
     3421 attgcgtatc cggggggcgc tggcaccgag aaaatggtgc agagcatgat caatgcattg
     3481 caggccgaag tcggtgagat tactcagcca gaacctgccc cggcacagcc tgatgaaacg
     3541 gttagcgaag cagatgcaga ggctaataaa gccattgaat atctcaataa cgtgatggat
     3601 atgcaaagca ctgacatggc ggagatccgt aacgcccggg gtaatgtccg ggaagcgatt
     3661 gcagcccttc aggctgccgg gcgttttgag gaaaacgaag agctggttaa tggcgctgct
     3721 cgccacctgg ctgatctgct ggtagcaatc cagaaagcgg gggtagcggc atgacactat
     3781 cagctattga gttaatggat ctcagcgata agttggatgc tctgatgtcc aaagcggcta
     3841 ccgcgagtgg catggagttg ctggatatca gcgatgaaat tgaccagatc atgcaacaga
     3901 tggggtacgg cgtgtccggc ggcagtagtg acgaggaaaa acaaccttcg gaacatgatg
     3961 gtgtgccaaa actgattgct gatttcctgg ctgataaatt cgtcgatcag agcactgatg
     4021 catttatcgg tacgttacag gacttgagtc aatatgttgg cacatacatc gacctggacc
     4081 aagttaaaca gcacacggcg gcatggatag ccgccaacat taaagaggca gcgtgacatc
     4141 ctccacgccc ttcggggcgt ggattcctgc tacgttcagg ctgtcgcctg aatcatttcg
     4201 gtgggttcct gcttcaacgg gcggcctgac tgcaccatcc ctccacaggc aag
//
LOCUS       DAZUFB010000221         4176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZUFB010000221 DAZUFB010000000
VERSION     DAZUFB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.222"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..226)
                     /locus_tag="ACHY3M_005039"
     CDS             complement(<1..226)
                     /locus_tag="ACHY3M_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFS4319754.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAK"
     gene            complement(223..519)
                     /locus_tag="ACHY3M_005040"
     CDS             complement(223..519)
                     /locus_tag="ACHY3M_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319755.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            complement(509..706)
                     /locus_tag="ACHY3M_005041"
     CDS             complement(509..706)
                     /locus_tag="ACHY3M_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319756.1"
                     /translation="MNSLPAGWARPLMAKKHHFFKTGDNISICRRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            complement(699..1031)
                     /locus_tag="ACHY3M_005042"
     CDS             complement(699..1031)
                     /locus_tag="ACHY3M_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319757.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIQMILS
                     SMEDKANE"
     gene            complement(1070..1255)
                     /locus_tag="ACHY3M_005043"
     CDS             complement(1070..1255)
                     /locus_tag="ACHY3M_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319758.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            complement(1252..1803)
                     /locus_tag="ACHY3M_005044"
     CDS             complement(1252..1803)
                     /locus_tag="ACHY3M_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFS4319759.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            complement(1807..2322)
                     /locus_tag="ACHY3M_005045"
     CDS             complement(1807..2322)
                     /locus_tag="ACHY3M_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319760.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(2322..2855)
                     /locus_tag="ACHY3M_005046"
     CDS             complement(2322..2855)
                     /locus_tag="ACHY3M_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319761.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            complement(2859..3401)
                     /locus_tag="ACHY3M_005047"
     CDS             complement(2859..3401)
                     /locus_tag="ACHY3M_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319762.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            complement(3499..4029)
                     /locus_tag="ACHY3M_005048"
     CDS             complement(3499..4029)
                     /locus_tag="ACHY3M_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS4319763.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            complement(4041..>4176)
                     /locus_tag="ACHY3M_005049"
     CDS             complement(4041..>4176)
                     /locus_tag="ACHY3M_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319764.1"
                     /translation="SILQYHSPELIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ
                     "
BASE COUNT         1083 a         1107 c          882 g         1104 t
ORIGIN      
        1 ttttggcggt caccgtaccc ggttttaagg cccggttaac cgggttctga cgcactttaa
       61 aaccgcgttt tttgaaaaca tccagcacgc gggataactc atccggtgac atatcccggc
      121 agctggtttt gccggtggct gccagcagcg cggatgtata agtctcatca tccagggcaa
      181 gctcgcgctt tgccacatga atggcggata tcagttttcc tctcataccg tatttacctt
      241 tttacggaca tggaaagcct tttctgcctc atcccaccgg gcatggtgtg ttggtggaat
      301 aatcattatg tgccagccat ctctggttgt tatccggcag aaaaattcac cgctatataa
      361 cacaatggcc tgctgaatga gcgcatcagc cgcccgttca accggatgtc ttttatcaaa
      421 aaagcagatg atttttcgac ataaccaccg ggtaataaca aatgcgccga aaaaggatgt
      481 gatatataag caccagaaca acagagaact attgtccatt atctttctcc ttattcagtc
      541 ttctgcgaca ttcggcacag tcatcagggc tttcaaatgt atccggctcg cgattatgag
      601 ccagatataa ccaccgccta catatactga tattatcgcc agttttaaaa aaatgatgct
      661 tcttagccat cagagggcgc gcccatccag cgggtagact attcatttgc tttatcctcc
      721 atactggaca aaatcatctg tattaatgtc gggcgtggag atatacgcat ttcattaagc
      781 gtctgaaggg cagcaacagc tgcctgttta tcggtgcctt tggcaatttc cgcaagagaa
      841 tgcacaatcc ataacggact taacgccacg actgaatcca gaatcatgtc atccataata
      901 atttctgaca catcccgctt atcacattcc ttatccataa aagaaacgat tttttccaca
      961 tcttccggtt tcatgcctcc ggcgataaga aatttgcgat actcataaga cgatcttaat
     1021 tcaatactca tattcatctc ctgtaactct gacaaggacg tgatttttct catttatgtt
     1081 ttccagtaat acggatatat atgtcgataa gaagattcat aataaacagg aagagcgaca
     1141 tcctgataaa gaaccaaata tctggcatca tggacaggtt tcctgtaatc gcaaatataa
     1201 aaccaacccc caacaccgga acgaaaattg cgaaaaatac aagtctcttt atcatggcgt
     1261 taactcccgt aatacagcgt cctcggcagt ccggcgatgg tgataaataa aattcaccgt
     1321 actctcggat aattcaaatt tctcgcctat ttcccggaag gtaagacgtc gtggtgattc
     1381 agcatcccgt aattcacgaa taagatgaac atcatcgtca ggtatacggg tgaagggcag
     1441 caattctccg tattttttca tgcttacgcc aatatatcgc gcccgattgt ggacagaatc
     1501 ccggtctcgc cccagatatt ctcctatttg cttaccggtc atcgttctgg cattttttcg
     1561 gataaattta tcttcatcat gtttaaatct tggacgtttg catttgagca actccgggta
     1621 agtaccgcgt aataaaacaa tccggttatg tacaccccag aaacttcttt ttaccctgac
     1681 agcaatatcc ttaaccgggg tggatggata aagcgcaatc agcagcgcat cttcttcagg
     1741 tgtccacgca cggacatgag caggtgcctg accttttcgc cccatgggtt gtaaaatcat
     1801 catacatcac cccctgactc gcttttcagc agcataaaac tcatcagaca gaatttcggt
     1861 cattttctga tttgcgggtt ttacgccgca ctcaaggtaa atcacatcgt caacgcagaa
     1921 ccacttcaca gggccaaaca tgattgccga gaaatcaata cccagccaga acaacagtgc
     1981 atcggttctg gcatacgtga cgggcgaata ttcacgccac agactgttaa gttcgtcaga
     2041 ggcaacgcgc agggctttgg gaatacgtga tgtgcgcggt gtgcagctcc agccattcga
     2101 cgcagtcggt tttcgccata agtcgcgatg aaaaggatat ttgtcatcca taaagcgcag
     2161 ccctttaaaa caaaagccac tgataccgga tacaaatacc gaccggcacg caacattcag
     2221 cacggcctca aggcgttttg cctcatcctt aactttctgg cagtcctgct gatatttttg
     2281 ccacgcggcc agcgcggaag ggtttgatgt tttaaagaac attacgccac ctccgcagat
     2341 atcccggcag gcgcggccgt ccggtcaaca atcaggtaac gcagaacgcg cgaggtgata
     2401 ttccagccgc ttaaaccgca gataatgaca cccaggcgta tgtcgatata cgccatcaca
     2461 aactggggca cgccttcttt catggcctgc tcatcaactt cagccaccac ataaacggtt
     2521 tcacaggtca gaacgccctg agcaaattcc cacgccattg acggaatatc atgcccctca
     2581 atccacggca gggattttct gaagctgcac cactgaacat catcagccgc accttcctgg
     2641 ctgcatttgc gacgaaccgg ctgcaccggg cggggacgcg gaatatcata aaaaccgtta
     2701 cattccgtca gcaccccggc atcgaccgca tcacgcagaa aataaaccat cgaggatggc
     2761 ggcatattca ttttttcagc cagaatgccg caggtcagac gcccgtaaat actcagccag
     2821 tttttaaccc cttcaagcac ttttgcatca atcatgaatt attcctccgt cagcgccaca
     2881 tattcagaat gtttaatctc ctggcattct tcaggaatac ccccttttat ttccctgcca
     2941 taagcgccta ctttaaacac cacgcacagg gcatcacggg agagcgacgg ggtccaccag
     3001 gagtccatgt taaacatgga ggggattctc attaccccca gcttttcaca gagccagaca
     3061 aggaaatcag gacgctttct taactcgcgt tcagcggctt caatcattgc ggcaatcgcc
     3121 ggtccttcag ggcatgactc atcaggtctg gcaataaagc acggtccgtc cccgataaaa
     3181 gccacatcat cgcgcttacg ccagcccgca ggcagagcac cagaaaaata aaactcactg
     3241 attacgtcca tggcaaacgg ctttaagcgc aatccggcag cgcctgtatt tttcagtaat
     3301 gcccgaagaa tactgttgcg tagcggtaaa acagtctgtt gatatttggt gaaatattta
     3361 tttgtctgca cacggtctaa tttgaaataa aaatatgcca tatatcctgt tccttatttt
     3421 cagtttacag gcgcaggcag tcccctgacg cgagcgccat acttaaaacg atgtgatatt
     3481 taaagattta atgcggtgtt atttattcag tccggcgtcc tgttcaaaag gctcgacata
     3541 aaaactttca gcgcctttat tcaccttaat accggcaatg cctttcactg catccggctc
     3601 cgccaggacc gcttccttgt tcacttcctc tttcgtgcgg atgaatcgct caagccccat
     3661 acggcgcagc atttcaatca caccttccac atcacggctg acgctgcatg atggatttcc
     3721 cagccgccat gacactgttc cggtggtcag attggctgtt ttggttttgc cgccgttcgt
     3781 cagctcatcg cggttggttt tgcaccagtc ctgaatcccc ttaaaaagca ctttgatttc
     3841 ttttttaagg ttttcaatct gcggcgtata acgggcggtg atttctgcca cttcatcatt
     3901 catcgctgtt tccaggcgca gcgcctctcg ctgaatatcg cccagggtgc ggatatcgcg
     3961 gctgacctct tcacgggtct gcggtgccgc ctctgctgcg gcctttaatt ttgtaacgcg
     4021 ttttgccatt ttgtttttcc ttattgcagt gtgcctgatg ctttatgcac gcggtggcgt
     4081 ttaatgatgg catccgcccc aaaagcctgt acagatgcct gaaattccgc tgagagaaac
     4141 tgaataagtt caggactgtg atactggaga atactt
//
LOCUS       DAZUFB010000222         4135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZUFB010000222 DAZUFB010000000
VERSION     DAZUFB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.223"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1916
                     /locus_tag="ACHY3M_005050"
     CDS             <1..1916
                     /locus_tag="ACHY3M_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS4319765.1"
                     /translation="ADCLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGT
                     AEPSDWDNAETHTRPSGNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPR
                     IMQLARRSTVTPGGKACQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLH
                     IAKKLLPFIPADAGILLVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDL
                     ISRTKAALAKNPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCT
                     GGSAAGVPWICGDTTYFWKQKNEQAYQAIYDGYKNKTDKNIHFVPLMTDENGANVPTN
                     NPAEDPDIESIGYYGSTWRNSAATWTSTDRASHFSSWARRGIISDRLATAVLTHAGRT
                     TVKADVPSSETEAPVPSPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDA
                     GGTGGKAMKLTKETGKSSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALY
                     WPVSSLPQGVTLTGDAGNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNE
                     WHTLAFRFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLI
                     DSIVVEVKKAVTE"
     gene            2063..2245
                     /locus_tag="ACHY3M_005051"
     CDS             2063..2245
                     /locus_tag="ACHY3M_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS4319766.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            2281..2526
                     /locus_tag="ACHY3M_005052"
     CDS             2281..2526
                     /locus_tag="ACHY3M_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS4319767.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHSPAENSTENEPQAVEQPEPVQPGTDGTMM"
     gene            complement(2565..3022)
                     /locus_tag="ACHY3M_005053"
                     /pseudo
     CDS             complement(2565..3022)
                     /locus_tag="ACHY3M_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3137..3337
                     /locus_tag="ACHY3M_005054"
     CDS             3137..3337
                     /locus_tag="ACHY3M_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4319768.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            3291..4028
                     /locus_tag="ACHY3M_005055"
     CDS             3291..4028
                     /locus_tag="ACHY3M_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFS4319769.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTRNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT         1148 a          935 c         1055 g          997 t
ORIGIN      
        1 ttgctgactg tctcatcagt tttaatacac gggattgcct gtgggtaagg ccagtatccg
       61 gtgatccgag tgtggaaatt accggagcgg gtattggtgc ggtcattccg ttaagtgctg
      121 atgttgccgg tactgctgaa ccgtcagact gggataatgc tgaaacacat acccgtccgt
      181 caggaaatga aactgcttcc agctcccctt cctggtatta cgtggtggtt cttgctggtc
      241 agtcaaacgg tatggcttac ggtgaaggtc tgccactgcc ggagacatat gaccgccccg
      301 aaccgcgtat tatgcagtta gcccgtcgca gtacagtcac gccgggaggt aaagcctgtc
      361 agtataacga catcattctg gccgaccact gtctgcatga tgtgcaggat atgagtggaa
      421 aaaatcaccc gaaagcggat gtggctaaag ggcaatatgg caccgtgggt caggggcttc
      481 acattgccaa aaaactgctg ccgtttattc cggcagatgc ggggattctg ctggttccct
      541 gctgccgtgg tggttctgca ttcacggcgg gtgctgacgg cacgtacagt gacagcacgg
      601 gtgcttcaga ggactcagcc cgctggggtg tggataagcc gctgtataag gacttaatca
      661 gccggacaaa agcggcactg gcgaagaacc cgaaaaaccg cctgcttgct gtggtgtgga
      721 tgcagggcga gtttgatatt gatgcgaagc cgacggagca ttctgcgctg tttctggcga
      781 tggtggaaaa attccgcgca gacctggctg aacaggcgga acagtgtacc ggtggcagtg
      841 ctgctggcgt tccgtggatt tgtggtgata ccacgtattt ctggaaacag aaaaacgaac
      901 aggcatacca ggcaatctat gacggctaca aaaataaaac agataaaaac attcatttcg
      961 tcccattaat gacagatgaa aatggcgcga atgtacccac caacaatccg gcagaagacc
     1021 cggatattga atccattggt tattacggct caacgtggcg taacagtgcc gccacctgga
     1081 catctacgga ccgggccagc catttcagtt catgggcacg tcgtgggatt atttccgacc
     1141 gtctggccac ggcagttctg actcatgcgg ggcgaaccac agtaaaagcc gatgttccat
     1201 cttctgaaac tgaagcacca gtgccgtcac cttcagaaac tgaagcagta accacaacac
     1261 tgctgtctta ccgtgtcagt gagtcagaag gaaatctgaa agcccagggc tgggagccgg
     1321 ctggcggtaa agctgaaatt atcagtgatg caggaggcac aggtggtaag gcaatgaaac
     1381 tgaccaagga aacaggtaaa tcatcctggt atctggatca tgatgccggt actggtgctg
     1441 aactgctgaa aaatggtggt ttaatcagtt gtcgttttaa agtaccgggc gacctggtgg
     1501 caaaccagta tgtcatggcg ctttactggc cggtttcctc tctgccgcag ggtgtcaccc
     1561 tgacagggga tgcagggaat aatctgctgg catcgttcta catccagaca gacgcaaaag
     1621 acctgaatgt gatgtaccac aatgcaaaag tggcaacaaa taaccagaaa ctgggaacct
     1681 ttggtgcatt tgataatgaa tggcatacgc tggccttccg ttttgcagga aataacagcc
     1741 ttcaggttat tccggttatt gatggtcagg atggtgccgc gtttaccctg acgcagtcac
     1801 cagtaggaac tttcccggtg gacaaactcc gcgtaacgga tatcacaaaa aatgcaactt
     1861 acccggtgct gattgacagc attgtggtgg aagtgaaaaa agcggtaaca gaataaaaaa
     1921 atcccgccgg tcatgatatg gctggcggga gctacctaac ccataaagga aaataaaaat
     1981 actcccgaag gagtggttat cagtcgcttc gatgttttta cccgataacg aattatgtag
     2041 tcaatatcaa aggtaaggtt atatgacatt cgttcataca atgctgcttt atttctgcgc
     2101 agtggtcagt gcgttatatc tggtgagcgg cggttataag gtcatcagaa attatattcg
     2161 ccgtaaaatt gatgatgcgg cagctgaaaa actcagtaaa acagcacagg caccgtcatc
     2221 cccaaacgac ccgaccccgc tctgataacg gggcaatata cctgcaaaag gagaatattc
     2281 atgccagaga ttaaaggcac ggttactgaa gagcttgtca aacaggcact ttattctgaa
     2341 gaagtgaacc gcgtgctgaa ggcgcaggtt cgcaaggatt ttgaggcaca aatcgacgca
     2401 tatgttgatg aagtgctggc ccgaatggtt ggccattctc cggctgaaaa cagcacggag
     2461 aatgagcctc aagccgtaga gcaaccagag ccggttcagc ccggaactga cggtactatg
     2521 atgtaacaat accgaccggc aggtttccct gccgggtgaa tttgttaatg cacgttttgt
     2581 gtactttccc tccggggaga acaggcagga catatcagca tcctgaaaca ggacgcattt
     2641 tggggtttgt atgtcttgcc attttgagat cgagaacggc gatagatgag ctgtaaggga
     2701 gtcttacagg cagggcaaac aggctcttta tccatgataa agatctcctt tttatgcatc
     2761 tctgctttac aggagaccgg ggaatatcat aggatacgac acgagctcga agtaaagcat
     2821 tgatgcgaat ttgttccagg tccagatagc tgtaactatc tggacctccc tttatgtagt
     2881 atcctgttga tttgaaacat actacatata cgcgcgatcc taccttcact gtataaaaag
     2941 tcaataaaat gatcatttta tgatcgtttt caccacctaa gtcttcgttt tctttaagta
     3001 aataatcacc gccctgaatc attgttgatg acttttcaat ctatttgttg tatcaattaa
     3061 acaaatcgaa tcgatcgttt ttgtcgatcg attgcatgag aaaggcatca acaacaaagc
     3121 aggaggttat gcggccatgt atcgaaacat acggtgccga cactgcaata agctactggc
     3181 acgggccagt ttcagttatc tggaagtaaa atgtccgcgc tgtaaaaccc tcaatcaaat
     3241 cacatctccg agcgccacag agcaccccac atacacaagg aaatcctgtc gtggggaaca
     3301 gaaagcaagt cacatcccgt atcatcagca cgcctgaact tattcgctat aacgacaata
     3361 tcgttggtta tggctcccgt gagttgcggg ttgagacaat aagttgctgg ctggccagac
     3421 tggtcatcgt caataaacat tacagccacc gtttcgtaaa taactcctac cttcatctgg
     3481 gcatattttc agaacgggag ctggttggcg taatgcaatg gggctatgcc cttaatccaa
     3541 acagtggtgc acgcgtcgta acgggcacgc ggaaccgtga atatatggag cttaaccggt
     3601 tatggctgca tgactgtatg ccgagaaact ccgaatcaag ggcaatcagt tacgcgctca
     3661 agctaatcag gcagctttat ccgcaggtac agtgggttca gtcgtttgca gatgagcgtt
     3721 gtggctgtct tggtgtcgtg tatcaggcaa gcaattttga ttatgtaggc agccacgaat
     3781 caacattcta tgaacttgat ggcgagtggt atcacgagat ttgcaggaat gccattaaac
     3841 gaggcggaca gcgaggtgaa catctgaggg ctaatatcga cagagccagt gttcataaat
     3901 tccgtcagtt tcgttacatc cggtttctga acaagagggc cagaaaacgc ctcaacacaa
     3961 agctattcaa agtccagcct tacccaaaac cacaaacagt taaaactggt ttaaaagaga
     4021 gtgagtgaac gcttaaaaac tcattctaaa acccaaaaga aaagcgccga tttagtatca
     4081 aataagttgc gtatcggcgt ttttcagcgg tatcaaatga aatgcaaatc ggtat
//
LOCUS       DAZUFB010000223         4117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZUFB010000223 DAZUFB010000000
VERSION     DAZUFB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.224"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1128
                     /locus_tag="ACHY3M_005056"
                     /pseudo
     CDS             1..1128
                     /locus_tag="ACHY3M_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1984..2355
                     /gene="ler"
                     /locus_tag="ACHY3M_005057"
     CDS             1984..2355
                     /gene="ler"
                     /locus_tag="ACHY3M_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFS4319770.1"
                     /translation="MNMETNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            2370..2588
                     /gene="escE"
                     /locus_tag="ACHY3M_005058"
     CDS             2370..2588
                     /gene="escE"
                     /locus_tag="ACHY3M_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFS4319771.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            2601..2915
                     /gene="cesAB"
                     /locus_tag="ACHY3M_005059"
     CDS             2601..2915
                     /gene="cesAB"
                     /locus_tag="ACHY3M_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFS4319772.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMNQRNRNNRK
                     IV"
     gene            2912..3511
                     /locus_tag="ACHY3M_005060"
     CDS             2912..3511
                     /locus_tag="ACHY3M_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFS4319773.1"
                     /translation="MTIFNKIDYNYYKLINYPIMMVHDEWLGDLTGVNQVSFRRLRET
                     SSTRNQLNKILRQEIQDKISGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYI
                     FSRLYRETLIREIGYQNVKQLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADN
                     PLWTIVKYLLPKSGEIKPTHINDLFLNRLNKILLPYETL"
     gene            3498..>4117
                     /gene="espL"
                     /locus_tag="ACHY3M_005061"
     CDS             3498..>4117
                     /gene="espL"
                     /locus_tag="ACHY3M_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFS4319774.1"
                     /translation="MKLYEFKNIKIDLVLTEDIIPEDKLQEIIQSDDIIKLARKKTYE
                     HLLRARRKSKELKTESRKKIARKMIVMRERIRKNNKIKLDKEVNQSIKWVKDIQAIEL
                     VLMQDIMNKVHLSLTNALHSLDTSSRINWDDLLNEVVRETLSNNNIVGAIKITKNPDI
                     KLDPGEANNIQLINDANTPHNKIIIENEYIRITLDPLEQISILLNS"
BASE COUNT         1405 a          701 c          914 g         1097 t
ORIGIN      
        1 tgttgcccgg caacacggtg tggcagccag ccagttattt ctctggcgca agcaatacca
       61 ggagggaagt cttactgctg tggccgcagg agagcaggtc gttcctgcct ctgaacttgc
      121 tgctgccatg aagcagatta aagaactcca gcgcctgctc ggaaaaaaaa cgatggaaaa
      181 tgaactcctt aaagaagccg ttgaatatgg gcgtgcaaaa aagtggatag cgcacgcgcc
      241 cttattgccc ggggatgagg agtaaacgtc gtcagtcgtt gtcgccgggt gtcgcatgag
      301 cagttgcaca tcattctcag acgaagtgat gactggaagg atggccgccg cagtcttcac
      361 actgatgata cggatgtgct tctccggata catcatatca tcggagagtt gcccacgtat
      421 ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg tatgtctgcg
      481 atcaatgcca aacaagttta ccggatcatg cgccagaatg cgctgttgct tgagcgaaaa
      541 cccgctgtac cgccatcgaa acaggcacat accggcagag tggccgtgaa agaaagtaat
      601 cagcgatggt gctctgacgg ggtcgagttc cgctgtgata acggagagag actgcgtgtc
      661 acattcgcgc tggactgctg tgaccgtgag gcactgcact gggcagtcac tacgggcggc
      721 ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg cttcggcaac
      781 gagcttccgg cgtctccagt agagtggctg acggataatg gttcatgcta ccggcctaat
      841 gaaacacggc agtttgcccg gatgttgggg cttgaaccga agaacacggc ggtgcggagt
      901 ccggagagta acggcatagc agagagcttc gtgaaaacga taaagcgtga ctacatcagt
      961 gtcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc attcgagcat
     1021 tataacgaaa ggcatccgca tagtgcactg ggttatcgct cgccacggga atatctacgg
     1081 cagcaagcca gtaatgggtt aagtgataac aggtgtctgg aaatataggg gcaaatccag
     1141 cgacaaagaa cagggttaat gaatctgtaa ccagtcccac cttagcgcta tgcacttttg
     1201 aatcacagat cttgctaatc gtttgtccga ctttaacaga gttaatgacg gcgatgatct
     1261 gatgccaagt aaaacggact tgatgtatgg cagatcctct ttcgccgaat ggatatgggc
     1321 aatacttctt aatacgaatg gtacggttat gcgggaagag actaacgcgg ttacttgttc
     1381 agctatttgt cccttgttcc tttttataat gcacccgttc caggttagtg ctggctgtag
     1441 cttatgtccg ggagacagct aatagatata tatactcgtc atacttcaag ttgcatgtgc
     1501 tgcgactgcg ttcgcttacc ccaatcactt acttatgtaa gctcctgggg attcactcgc
     1561 ttgccgcctt cctgtaactc gaattaagta gagtatagtg aaacggttca gcttggtttt
     1621 tattctgttt tatttgttta tgcaatgaga tctatcttat aaagagaaac gcttaattaa
     1681 atggaaatgc aattattaaa gtcgtttgcc aacgagattg tttttcttct atatcattga
     1741 ttttaaatgg attttaaaaa tatatgattt ttttgttgac atttaatgat aatgtatttt
     1801 acacattaga aaacagagaa taataacatt ttaaggtggt tgtttgatga aatagatgtg
     1861 tcctaatttg atagatgaac gttatctcac ataatttata tcatttgatt aattgttgtc
     1921 cttcctgata aggataaggt cgctaatagc ttaaaatatt aaagcatgcg gagattattt
     1981 attatgaata tggaaactaa ttcacataca acaagtccat acattcagct tatagagcaa
     2041 attgcagttc tacagcagga agcaaagcga ctgcgagagc aggaagttca aagtgtaatt
     2101 gagtcgattc agaagcagat tacttattac aatataacct tacaagagct gggatatact
     2161 aatgtgcctg atgatggact cgctcgccgg aactcatcga aaggtgttta ctatcgcaat
     2221 gaagaagggc agacctggtc gggcgtaggc cgacagccac gctggcttaa agaagcactg
     2281 ttgaatggaa tgaagaaaga agattttctt gtgaaggaca ctgaggaaga aataataccg
     2341 ctgaaaaata tttaacataa aataattaaa tgataacgat aactgagctg gaagacgaaa
     2401 taataaaaaa taaagaagcc gcaaatgttt ttattgaaaa aataaacgac aaaaagaacg
     2461 aaatccatga aaaaatgaaa caccctttgg ataaagttac ctacaatgaa gcaaaggaac
     2521 tgctcattgc gtgtgatgcg gcaattagga ctatagaaat aatgcgaatc aggattaata
     2581 ataaatagag gatgagtatt gtgagccaaa caagaaataa agaactatta gataaaaaaa
     2641 taagatcaga aattgaggcg ataaaaaaaa taatcgccga atttgatgtc gtaaaagaaa
     2701 gcgttaatga gttaagtgaa aaagcaaaaa cagatcctca ggcggcagaa aaattaaaca
     2761 aattaattga ggggtacaca tatggtgaag agcgaaaatt atatgattct gccctaagta
     2821 agattgaaaa actaatagag acactgagtc cagcaagatc taaaagccaa tcaacaatga
     2881 atcaacggaa tagaaataat agaaaaatag tatgacaatt tttaataaaa tagactataa
     2941 ctactataaa ttaattaatt atccaatcat gatggttcat gatgaatggc taggtgattt
     3001 aaccggtgtc aatcaggtca gttttcggcg tttaagagaa actagttcta ctcgtaatca
     3061 actcaataag atattgcgtc aggaaataca agataaaatt tctggtgtgg aattatctga
     3121 cataaataag gaaggttttt tatatcagtc aattgggaaa ataagattgc ttgctctaag
     3181 tagtgcatta tttgacattc aatgccctga ttatattttc tcacgattat atcgagaaac
     3241 tctgattaga gagataggat atcaaaatgt gaagcagtta tcattctatt ggcagggagg
     3301 gcaatgtaaa ccagaatatg gtgaagaacg gttttgtgct gagttaataa aatacggtgc
     3361 aggaaatttg gagtggctat ttgctgataa tcctttatgg accatagtca aatatctttt
     3421 accgaaaagt ggagaaataa aaccaactca tattaatgat ttatttctta acagacttaa
     3481 taaaatttta ttgccttatg aaactttatg aatttaaaaa tataaaaatt gatttagtgc
     3541 ttacagaaga tatcatacca gaggataagt tacaggaaat tattcaatct gatgatatta
     3601 tcaaactggc tcgaaaaaaa acatatgaac atttactgag ggcgaggagg aaatcgaaag
     3661 aattaaagac agaatccaga aaaaaaattg ccagaaaaat gattgtaatg agagaaagga
     3721 ttcgaaaaaa taataaaata aaattagata aagaggttaa ccaatcgata aaatgggtta
     3781 aagatatcca ggctatcgag ttggtcttaa tgcaggacat aatgaataag gttcatttgt
     3841 cactgactaa tgcattacat tctttagata catcatcaag aattaattgg gatgatttac
     3901 ttaatgaagt tgtaagggaa actctatcta ataacaatat tgtcggagct attaagatca
     3961 ctaaaaaccc agacattaaa cttgatcctg gcgaggccaa caatatacag cttattaatg
     4021 atgcaaacac accgcataat aaaattataa tagaaaatga atatattaga ataactttag
     4081 acccattaga gcaaataagt attttattga attcatt
//
LOCUS       DAZUFB010000224         4113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZUFB010000224 DAZUFB010000000
VERSION     DAZUFB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.225"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..1728
                     /gene="dpiB"
                     /locus_tag="ACHY3M_005062"
     CDS             70..1728
                     /gene="dpiB"
                     /locus_tag="ACHY3M_005062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFS4319775.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1697..2377
                     /gene="dpiA"
                     /locus_tag="ACHY3M_005063"
                     /gene_synonym="citB"
     CDS             1697..2377
                     /gene="dpiA"
                     /locus_tag="ACHY3M_005063"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFS4319776.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="ACHY3M_005064"
     CDS             complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="ACHY3M_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS4319777.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1133 a          959 c         1051 g          970 t
ORIGIN      
        1 ctatgtattt tgattcaaca ttttaattac atccgtcaaa gaggctcggg acaacccgca
       61 aggaaaacaa tgttgcagct taacgagaat aaacagtttg catttttcca aagactggca
      121 tttccgctgc gtatcttttt gctgattctg gtgttctcaa tatttgtcat tgcagccctg
      181 gcgcaatatt ttacggccag ttttgaggac tatttaacgc ttcatgtacg cgacatggca
      241 atgaatcagg cgaaaattat tgcctccaat gacagtgtca tctctgcggt gaaaacgcgt
      301 gactacaaac ggctggcgac catcgctaac aaattacaaa gagataccga ttttgattat
      361 gtggtgattg gggaccggca ctcgatccgc ctttaccatc ctaatccgga gaaaattggt
      421 tatcctatgc agttcaccaa acagggcgcg ctggagaaag gggagagcta cttcattacc
      481 gggaaagggt caatggggat ggcgatgcgc gccaaaacgc caatctttga tgacgatgga
      541 aaagtcatcg gcgtggtgtc gattggctac ctggtgagta aaatcgatag ctggcgggct
      601 gagtttttat taccgatggc aggcgtgttt gtcgtactgt tagggattct gatgttgctg
      661 tcgtggttcc tggcagcgca tatccgtcgg caaatgatgg gcatggagcc aaagcaaatc
      721 gcacgcgtgg tccgtcagca agaggcgctg tttagttcgg tttatgaagg gctgattgcg
      781 gtggatccgc atggttacat taccgccatc aatcgtaacg caagaaagat gctggggctg
      841 agctctcccg gacggcaatg gttgggtaaa cccattgctg aagtggtcag gcccgccgat
      901 ttctttaccg aacagattga tgaaaaacgt caggatgtgg tggctaactt taacggtctg
      961 agcgttattg ccaatcggga agctattcgt tcaggtgatg atttgctggg ggccattatc
     1021 agctttcgta gtaaagacga aatttccacc ctcaatgcgc aactgacgca aataaaacaa
     1081 tacgtcgaga gccttcgtac attgcgacac gagcatctca attggatgtc gacgctcaat
     1141 ggtctgttgc agatgaaaga gtatgatcgc gtgctggcga tggtgcaggg ggagtctcag
     1201 gcccagcaac agcttattga cagcctgcgt gaggcgtttg ccgatcgcca ggtggcgggg
     1261 ctgctttttg gtaaagtgca gcgcgcccgg gaactggggc taaaaatgat cattgtcccc
     1321 ggtagccagc tttcgcaact gccgccagga ctggatagca ccgagtttgc agccattgtg
     1381 ggcaatttac ttgataacgc cttcgaagcc agcctgcgta gcgatgaagg aaacaagatc
     1441 gttgaattat tcctcagcga tgaaggcgat gatgtggtga ttgaagtcgc cgatcagggc
     1501 tgcggcgttc cagagtctct acgagacaaa atatttgagc agggggtcag tacgcgtgct
     1561 gacgagcccg gtgaacatgg cattgggttg tacttgattg ccagctacgt aacgcgctgc
     1621 ggtggtgtta tcactctcga agataatgat ccctgcggta ccttgttttc aatctatatt
     1681 ccgaaagtga aacctaatga cagctccatt aaccctattg atcgttgagg acgaaacgcc
     1741 gctggcagag atgcatgcgg aatatattcg tcacattccc ggattcagtc agatattact
     1801 ggcgggaaac ctggcgcagg cccgaatgat gatcgagcgt tttaagccgg ggctaatctt
     1861 gctcgataac tatcttcctg acggcagagg gattaattta ttgcatgaac tggtgcaggc
     1921 gcattatccc ggcgacgtgg tgtttaccac tgcagccagc gatatggaaa cggtgtctga
     1981 agccgtacgt tgtggtgtat ttgattatct cattaagccc attgcctatg aacggctggg
     2041 gcaaacgcta acccgtttcc gccagcgtaa acatatgctg gaaagtattg atagcgccag
     2101 ccagaagcaa attgatgaga tgtttaatgc ctatgctcgc ggtgaaccta aagacgaact
     2161 gccgaccggc attgatccct tgacgctaaa cgcggtgcga aaactgttta aagagcctgg
     2221 tgtgcaacat acggcagaaa cggtggcgca ggcactgacc atcagtcgca ccactgccag
     2281 gcgttatctt gaatattgcg ccagccgcca tctgattatt gctgaaattg ttcacggcaa
     2341 agttggcaga ccgcaacgca tataccacag tgggtgacat gataaagccg gaggaaactt
     2401 gcctccggca ttactattta cttgcctgtg accgctgctg ccgttcctgg caccatcagc
     2461 tctgtagcaa cgataacaat caccaaacca acaagcaccg gtaccgaggt gcgttttacg
     2521 acttcaaacg gcgagatctt cgccatcccg gcaaccgcaa cgactacgcc agaaacgggc
     2581 gaaagggtac ggcccaggtt tgacgcctgc agcatcggga tagtcaaata cgccgggtta
     2641 atgccggaag agtgcgccag tttcgggatc atctcaacaa acgcataaaa cggcgcattg
     2701 cctgaaccgg tcgtgactgc cgccagcatg gtcagaatca ccaataccag catcaggatg
     2761 atactcgccg aaccaaacga ggtagcgata gaaatcagac tttgaataaa gccaatggtg
     2821 ctaagcccct gagcgaatac cccagcggca accagcagca tcaccacgtt agcaaacgca
     2881 tctgccattc cgcgataagc cacttccaga ccagagaaaa ctttctgggt attaaagctg
     2941 cggaggaact ccagaatgga ggcaatcagc atacaaatca ccagaatagt gatgatgtgt
     3001 aattgcggac cccatttacc gtcaaaaatc agtacaccga tgatcggcgt gaacggcaaa
     3061 atggcataaa acgcaggagc agtggtggtg atttcactga catctaacat ttcatgagag
     3121 atgtgctctt ttttatccag ataacgttgc cagaagaagt gggcgatcgc catgccgata
     3181 attgcagcaa ttgagatagg cagcgtcgtt ttgaaggcga agtcaatcag cgacatttcg
     3241 gaagcttgcg ccgccagcac cacatcccct gaagtcggtg cgagaataat cgccgccggg
     3301 gaggcacaaa tggcagcagc tgcgccacga ctgataccaa cgtttaccat caccggaaat
     3361 agggttgcca tcagcaaaac acccagaccg gttgcggaag agacggccag agacatcaga
     3421 caggcgacaa aataggcggc aatcatcagc aggtaagggg agttaatata ctgcaatggt
     3481 tttgacgcca gcttgaccac catatcattc gcgccgatat gggtcatgta agcggcaaat
     3541 ccacacagca tcataatcat catgccgagg tctccgccgc ggctcattag taatatttta
     3601 acgtattcaa cgatatccgt ggcgctgtag cctgttgaag cctggctgga cggtaacact
     3661 ttgtgcccca taatggcact gataatcagc aataacaggc caccgacaaa taacacacca
     3721 gtggcggaat accctttaat gatgtagcga gctacaccca caataaccac aaccccaata
     3781 aggagttcaa tgaatgtcag cataattttt cctgtctcca ggccccaaag taaataataa
     3841 aaaattccta aagcttaagg aaaaaatatg cccaataaat tggcgatgaa tgctgattaa
     3901 aatcaagaaa aactgccatt aagacattga agttgctgtt tttatacata gataacaaaa
     3961 cgcatattta gcggttggca attatcacat aataatgtgt gtaaattgat ggtggattga
     4021 tgcaaatttg ttaattgcaa tggtgttagc tgcggctgtg cgctcaaaaa ataatctaac
     4081 atgagcatag gttgacgatt tatattatat act
//
LOCUS       DAZUFB010000225         4110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZUFB010000225 DAZUFB010000000
VERSION     DAZUFB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.226"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..786)
                     /locus_tag="ACHY3M_005065"
     CDS             complement(<1..786)
                     /locus_tag="ACHY3M_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFS4319778.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVDLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYN"
     gene            1101..2389
                     /gene="shiA"
                     /locus_tag="ACHY3M_005066"
                     /pseudo
     CDS             1101..2389
                     /gene="shiA"
                     /locus_tag="ACHY3M_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
     gene            2491..3945
                     /gene="amn"
                     /locus_tag="ACHY3M_005067"
     CDS             2491..3945
                     /gene="amn"
                     /locus_tag="ACHY3M_005067"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFS4319779.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          989 a          983 c          936 g         1202 t
ORIGIN      
        1 attataacaa tccactaata ccctggcttt atcttcccct tttggtccat gaacgatcac
       61 tattggtata tctgtttcac tttgaatttt tgctattaga ttttctgcaa tcgataatga
      121 aaatgtacgt tcctgcgagc taccttctaa attaagcgca atgtaagatc ctaacgatcg
      181 catttcctcg cgcacctcat cgagtacatc ctcacttagt ggcaattcat atattggcct
      241 gactgctgga aaacccgcct cacgcatcat aaatgcccat gtcatgggta cgggagcccg
      301 gagattctga tccatccggg acgcgttctt gcacaaaggg gagtagcact tcatggttaa
      361 atcaacaacc tgaaaattcg tttttgcttt caactgactg ataaatatca tcgttttcag
      421 gttcttttta cgcatcgcct ctatacaaag atccggcgta ccgtattgct gtgttatgtt
      481 ctttgctaaa tcttttattt cttttaatgt tgcgtgatcc tgcatagtca ttgtgactaa
      541 tgttaattta gtcttttcaa gtttaagtgc attaaacact tctaaattaa ttgtcgacgt
      601 aacaattaaa agatgcttaa ttttatgcaa ttcaagcgcc cgaataacag gaaagatggc
      661 catagcatcg ccaatctggt cgggaatatg gatgacaaca aagtctgttt tttcaatatt
      721 gaaattataa gctttataat cgtagtaact aaatgcaata cgtctcaaca atgatgctaa
      781 aaacatacct aacctcgcct ccctactggt tataatacaa tgcagtctat cagactcatc
      841 agggtgccat tttgtgcata tgcggacttt tatgtttcat atctctaacc tgtgggtcct
      901 ctgcttaatc cttaaacaac accagcaact cctgcgcttt catcttccat cgaatttttc
      961 atgttgccgc taatcagcca taaaaacatt tgcagatgcg ctctgtcgag gtagtctcat
     1021 aaggttcgtt tatagatcga cggcaatgtg agttaccttt tccatactaa ttataaaaag
     1081 acagtacaaa caggatcatt atggactcca cgctcatctc cactcgtccc gatgaaggga
     1141 cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag cttcgctggt gccgtcgtcg
     1201 actggtatga ttttttactc tatggcatca ccgccgcact ggtgtttaat cgcgagtttt
     1261 tcccgcaagt aagcccggcg atgggaacgc tcgccgcatt tgccaccttt ggcgtcggat
     1321 tccttttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga ctgggacgta
     1381 agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg attggtattc
     1441 ttccttcatt ctcgaccatt gggtggtggg cacctatttt gctggtgaca ctgcgtgcca
     1501 ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg
     1561 caccgaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac ggtgtaggtt
     1621 tactgctttc aaccggactg gtttcattga tcagtatgat gacgactgac gaacagtttt
     1681 taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg ggagcattgt
     1741 gggtgcgcaa tggcatggag gagtccgcgg aatttgaaca acagcaacat tatcaagctg
     1801 ccgcgaaaaa acgcatcccg gtgctttcct gaagattatt gcgctacgac tgtgcgaatt
     1861 gctgacgatg tacatcgtta ctgcctttgc acttaattat tcaacccaga atatggggtt
     1921 accgcgcgaa cttttcctca atattggttt gctggtaggt ggattaagct gcttgacaat
     1981 tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata tcacaggtgc
     2041 gttaatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac aatctatttt
     2101 ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg tggtgtgtgt
     2161 gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg gtgctggagt
     2221 cggttatcag gtcgccagtg tggttggcgg tggatttaca ccttttattg ccgctgcact
     2281 catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg ctggatgtct
     2341 gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat agtctggcga
     2401 agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata ctatcggatg
     2461 tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg tctgacccca
     2521 gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc attacgcaac
     2581 gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt
     2641 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaaccaa tccccccaaa
     2701 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
     2761 actctctttc gttcgtatct caatgaacaa cttacgttgc tgtatcagga ttatggtgcg
     2821 catatctcgg tgcaaccctc gcagcatgaa atcccctatc cttatgtcat cgatggctct
     2881 gaattgacac ttgatcgctc aatgagcgct gggttaactc gctacttccc gacaacagaa
     2941 ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga attctccccg
     3001 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc
     3061 ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac acgttatgtg
     3121 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
     3181 ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga agaagccatt
     3241 tccgaccttg catggaaaaa acatcagatg ccagcatggc atttaattac cgccgatggt
     3301 cagggtatta ctctagtgaa tattggcgtg ggaccgtcaa atgctaaaac catctgcgat
     3361 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
     3421 gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga tgaccacgtt
     3481 cttgatgcgg ttctgccgcc cgatattcct attccgagca ttgctgaagt gcaacgtgcg
     3541 ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa acagcggcta
     3601 cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta ctcagcttct
     3661 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     3721 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc agataaaccg
     3781 ttgcatggcg agattaaact tcccggccag gctaaccgtt tttatgaagg cgctatttcc
     3841 gaacatctgc aaattggcat tcgggcgatc gatttgctgc gcgcagaagg cgaccgactg
     3901 cattcgcgta aattacgaac ctttaatgag ccgccgttcc gataataagt tctgaacctt
     3961 cttatcagaa atagatgtga aggagtaagt aagacacctg gcaaatagcc tgcaataatc
     4021 gtgggctgtt tgcttccttg ggcggatacg tgttttatta tcgtcttaat gatttccaca
     4081 tattaaaagc aagtatgctt tcaaaacaca
//
LOCUS       DAZUFB010000226         4066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZUFB010000226 DAZUFB010000000
VERSION     DAZUFB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.227"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..752
                     /locus_tag="ACHY3M_005068"
     CDS             144..752
                     /locus_tag="ACHY3M_005068"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFS4319780.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            850..2241
                     /gene="selA"
                     /locus_tag="ACHY3M_005069"
     CDS             850..2241
                     /gene="selA"
                     /locus_tag="ACHY3M_005069"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFS4319781.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2238..>4066
                     /gene="selB"
                     /locus_tag="ACHY3M_005070"
     CDS             2238..>4066
                     /gene="selB"
                     /locus_tag="ACHY3M_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFS4319782.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          910 a         1027 c         1204 g          925 t
ORIGIN      
        1 atcagataga aacaattagg aaaatattta tatcaccatg ataaatatgt gatttatttg
       61 gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt
      121 tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac
      181 gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc
      241 cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg
      301 tgaccgaaga gggcgaatgt tggtttgatt cgccgatcat cgctgaatat attgaattaa
      361 tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa
      421 ttgaggcact ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc
      481 gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca
      541 gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc
      601 tggcgactat cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc gttgcgccag
      661 gctggtgtgt cgatcgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa
      721 gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc
      781 acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc
      841 aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc
      901 ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg
      961 gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg
     1021 cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaagcgcag
     1081 agcgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga
     1141 gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc
     1201 ctcgagtacg atctggacga cgctggacgc ggacatcgcg atcgtgcgct ggcgcagttg
     1261 ctgtgccgca ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg
     1321 ttattgatgt tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaactg
     1381 gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta
     1441 cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt attgtcaggc ggtgaatgaa
     1501 aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa
     1561 gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact
     1621 gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg
     1681 ccgcaggagt tgattgcggc gggcgtcagt ctggtgagtt tctccggcga caagttgtta
     1741 ggcgggccgc aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc
     1801 cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg
     1861 ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt
     1921 acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggcccc ccttgccgca
     1981 cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt
     2041 tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc
     2101 ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt
     2161 cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt
     2221 ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa
     2281 ccttattgca ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg
     2341 gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctggtt
     2401 ttatcgacgt tcccggtcat gaaaaatttc tttccaacat gctggcgggc gttggtggta
     2461 tcgatcacgc gctgttggtg gtggcgtgcg atgacggtgt gatggcacag acccgtgagc
     2521 atctggcgat tttgcagctg accggtaacc cgatgctgac agtggtgctg accaaagccg
     2581 atcgcgtgga cgaagcgcgt gttgatgagg ttgaacgtca ggtaaaggag gtgctgcggg
     2641 aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cgaggaattg
     2701 atgccctgcg cgagcatctg cttcagttac cagaacgtga acacgccagc caacatagtt
     2761 tccgcctcgc gatcgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta
     2821 cggcgttaag cggggaagtg aaggtaggcg attcactatg gctaactggt gtaaataaac
     2881 cgatgcgtgt acgtgcgctg catgcacaaa accagccaac agaaaccgcc catgccgggc
     2941 agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact
     3001 ggctgcttgc cgatgcgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc
     3061 atacgccgct gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg
     3121 gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc
     3181 tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg
     3241 cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat
     3301 ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac
     3361 gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc
     3421 gcgaattgct gcaacagcct ggttatattc aggctggtta tagcttgttg aatgcgccgg
     3481 ttgccgcccg ctggcagcgg aaaattctcg acacattagc gacttatcat gagcaacatc
     3541 gcgatgaacc tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg
     3601 aagcgctggt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc
     3661 acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct
     3721 ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag
     3781 agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa
     3841 taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca
     3901 atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc
     3961 gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct
     4021 ttacgcgtcg tcgtggaaat gatcatttat tgcgcgacgc attatt
//
LOCUS       DAZUFB010000227         4031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZUFB010000227 DAZUFB010000000
VERSION     DAZUFB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.228"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..701
                     /locus_tag="ACHY3M_005071"
     CDS             <1..701
                     /locus_tag="ACHY3M_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4319783.1"
                     /translation="LWTFIWAICATIFTFSFGFILALALAKKNIRFAKVWRLIFILPY
                     AIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAVCVWVGAPYF
                     MLLIAGALTNISRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFG
                     AIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNFQQYHIASVISIIIFIFLSVIAIW
                     QFRRMKSFKDDVGM"
     gene            704..1537
                     /locus_tag="ACHY3M_005072"
     CDS             704..1537
                     /locus_tag="ACHY3M_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4319784.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVSFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            1557..3356
                     /locus_tag="ACHY3M_005073"
     CDS             1557..3356
                     /locus_tag="ACHY3M_005073"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4319785.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEELGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            3378..>4031
                     /locus_tag="ACHY3M_005074"
     CDS             3378..>4031
                     /locus_tag="ACHY3M_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4319786.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTD
                     "
BASE COUNT         1156 a          683 c          853 g         1339 t
ORIGIN      
        1 ctctctggac atttatttgg gcaatttgcg cgacgatttt taccttcagt tttggcttta
       61 ttctggcttt agcactggct aaaaaaaata tacgtttcgc aaaagtctgg cgattgatat
      121 ttatactgcc atatgccata cccacatttg taacgttact tattttccgt cttcttttaa
      181 atggaattgg tccggttaac tcgtttctga acagtatcgg aatgaattca gttgattttc
      241 tgtcggttcc attaaatgca aaaataacag taattgccgt ttgtgtttgg gtaggggcac
      301 cgtattttat gctattgatc gcgggtgcat taacaaacat atcccgagat ttgtatgagg
      361 ctagtgaggt cgatggcgca actaaatggc aacagtttaa ggaaattaca ttgccaatgg
      421 tgttacatca ggttgcgcca actctggtaa tgacatttgc atataatttc aataattttg
      481 gtgcaattta tcttctgact gaaggtggtc cgactaatcc ggaatacaga ttcgctgggc
      541 ataccgatat ttttattacc tggatttata aactcacatt gaatttccag caatatcata
      601 tagcttctgt aatctccatc ataatattta tcttcttatc ggttatagca atatggcaat
      661 tcagacggat gaaatcattt aaagatgatg tagggatgtg acaatggata aatttttacg
      721 taaaatatca acgctcttag tttatctttt tttaatctgc aactccattc ttgtgcttgg
      781 gccagtgata tggacgataa tggcatcttt taaaaaaggg aataatctat ttagttcaac
      841 atttagtggc atcgagttta catttgacca ctacataacg ttgttcacgg atacgccata
      901 tatgcagtgg tatttgaaca ctttcattct tgctaccgct aacatgttaa tttcactgtt
      961 cgttgtgacg atgaccgcct acgttttttc aaggtatcga tttcgtggaa agcgtaatac
     1021 tttaatgagt attcttgtat tgcaaatgtt tcctgcattt ttatcaatga ctgcaattta
     1081 tatcctgcta tcaaaggcca atttgataga cacatatact gggttgcttc tggtttatgt
     1141 aaccggttca ttgcccttca tgacgtggtt ggtaaaaggt tattttgatg ctattccaac
     1201 ttctttggat gaggccgcta aaatagatgg agcagggcat ctgactattt ttattcagat
     1261 aatattgcca ttggctaagc cgattctcgt tttcgttgga ttagtttcgt tcactggacc
     1321 atggatggac ttcatcttgc caacgttggt gcttcgtagt gaggataaaa tgaccctggc
     1381 gattggaatt tttagttgga ttgcttctaa ttcagcagag aattatacgc tttttgctgc
     1441 tggggcgctt ttagttgcag tacctatcac attgttattt gtattcacac agaagcatat
     1501 aactactgga cttgttagcg gtgccgttaa ggaataattt ttagggatta gtatttatga
     1561 ttacagttag ttcgttaaaa cactcagcaa aaagtgaaga tagctatgcc tatgataacg
     1621 aaactttgca tgtacggtta cgcacattaa gaggagaagt tgataaagtt attctgtgga
     1681 taggcgatcc atataattgg gctgaaggtg gtcttgacgg cggtaacatg gccgggacag
     1741 aagcgttcgg ctggattggc ggcaacgaga tcgagatgga acaagaagct gtcaccgaat
     1801 ttcatgatca ctggtttgca gtgttcaaac ctcaaaaacg ccgttgccgt tacggtttta
     1861 tactttttgg gaaagaggga gagaagtttc tttttggtga gaaaaggtgt gttgatatca
     1921 gttcaccgga ctgcgaagaa cgagagttaa gccgcttaaa taactttttt tgcttccctt
     1981 atcttaataa aattgacgtt ctaaataccc cctcctgggt aaaaaatacg gtatggtacc
     2041 aaatttttcc agaccgtttc tgtaacggca gacctgaaat atccccagaa ggggtagaac
     2101 cgtggggcag tgcaccgact tcattcaact ttatgggtgg ggatctgtgg ggggttattg
     2161 ataaactcga ttatctggaa gaactgggta ttaatgggat ttatttttgt cctattttta
     2221 cctcagcttc caaccataaa tatgacacca tcgatcattt tagtgttgat ccacatttag
     2281 gtgggaatat tgctttccat actttgattg aagaagcgca taagcgtgga ataaaaatca
     2341 tgttggatgc agtattcaat catttaggcg ctgactctcc aatatggctt gacgttgtac
     2401 gcaatggtgc caattcacgt tacgccgatt ggttctggat tcataaattt cctgtttatc
     2461 ccgatactcc aaaaagtgaa tgggatttta aaaactttaa ctatgaaacc tttggcaatg
     2521 taattgaaat gcccaagcta aatactgaga atgaagagtg ccgtgaatac ttacttagta
     2581 tagttcgcta ttggacgcaa aattttgata ttgatggttg gcgtctggat gttgcgaatg
     2641 aagtggatca tcatttctgg cgagatttca gaaaggtgat taaagatatc aaacctgaat
     2701 gttatatttt gggtgagata tggcatgagg gcaccccctg gttgcgtggt gatcagtttg
     2761 attcattaat gaattatccc ttaacatatg ggattattga ttattttgcg ttacaggata
     2821 caacaaaaca agaatttatg acatccgtta cacgttctta tctttgttat cctaaaaata
     2881 taactgaagt tatgtttaat cttctggata gtcatgacac cgcgcgtatt ttaagcgttt
     2941 gtttgaatga taaacgaaaa gttaaactcg cctatttatt tatgttaact caagcgggct
     3001 ctccttgtat ctactacggt agcgaaatag gaattgatgg ttttaaatca atgacgcttg
     3061 agaataatag aaaatgtatg atatgggatg aaaacaaaca agatcttgaa ttacggcaat
     3121 ttatacgttg gttaatacgt ctacgtaaaa aacatccaca atggtgtgtg gcatcaatac
     3181 aatggaaaga tgttgaacat cctacggtga tcgcttatca acgcgataac atcactttct
     3241 tcttaaataa tagtgaagat actgccaact ttatttatga cggaagaagt atggagatct
     3301 ccggtttcag ttatgaaatt gaaggtttac ctgctgctga tctttatgat ttttaaattt
     3361 caattaggat gtttgttatg ataattgatg cacacactca tattggtgac tttgtgaaaa
     3421 tccgtatgcc agaagatgtt tttctggcat ctctggacaa gtataatatt gattttgcat
     3481 tatgttcttg tggatctgca gtagaagttg accatgacca gaacccgatt ccggatgaag
     3541 atcaagttac acagcatgat aataatgaac gtatgctcag acttgttcgt caacattcaa
     3601 agcgaattgg cgcgtttatg tggattaagc ctcgtctgga aagttgtgat caagattttg
     3661 aagatatgat tgccagcaat cgagatatca tttatggcat caaagttcac ccatatcatt
     3721 caaaaatggc tttcaattca gataaagttc aggaatatat acgccttgct caaaaatatg
     3781 atctcccggt agttagccat acttctaatg attatgaatc cagcccacag ttggtttatg
     3841 agatggcact taaatatcca aatgtgaatt ttgttatgtg tcatatgggg ctcgcgacag
     3901 ataatcagga agcgatcgaa ttgattgcaa aacttcctaa tctctatggc gatactgcgt
     3961 gggttagggc ggatatggtt taccagtcga taaaaatctg tggtagtgat aagattttat
     4021 ttggtaccga t
//
LOCUS       DAZUFB010000228         4022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZUFB010000228 DAZUFB010000000
VERSION     DAZUFB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.229"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2553)
                     /locus_tag="ACHY3M_005075"
     CDS             complement(<1..2553)
                     /locus_tag="ACHY3M_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFS4319787.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIR"
     gene            complement(2645..3145)
                     /locus_tag="ACHY3M_005076"
     CDS             complement(2645..3145)
                     /locus_tag="ACHY3M_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFS4319788.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3175..3891)
                     /locus_tag="ACHY3M_005077"
     CDS             complement(3175..3891)
                     /locus_tag="ACHY3M_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4319789.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
BASE COUNT         1105 a          939 c          815 g         1163 t
ORIGIN      
        1 acgaatgctg gcgcgtttct ttacaacact cagtggacac ttaaggaatt gctgcccgga
       61 aagcagatag agctcttttt cgctgccgtt aatttcaagt gtaaatccgc catcttcagc
      121 taccagtaac atggcgctgt tcagccccac aatcgggaat tttttcaccg ctgccggcag
      181 taataaacga ccgatatagt tgtagttaaa gctggcagac acttcgcgat agcgcagctt
      241 gccaggcaac aacattaatt cacgattccc tgcaccgtta atgatgtttg ttgtcccctg
      301 ctgattgccc tggctactgt cgttaacctc aacaaaacca gactgaaatc caggataagg
      361 gaacaacaca ctgtcacttt gaccaagctg caaagttcgg ctgcctgcca cccgcgcttc
      421 gagttgcaga tcctgactct cttcatcctg ttcagagaag cctttcacat ccaccagtac
      481 cgcacccagc aaaccgtctg taccactggc tcccaaatta acgccatagc gactgacagc
      541 cagcgtagag ctataagtgc ccgtaaaggc cgaaagatga tcatgattct tacggtcata
      601 actatcagat acggtagcgt agacatttcc gtattgccca tcccaacgcc cgttaaccgc
      661 gctgtttatg gtatcggtgt tcaggcctcc gacggttacg ccaagttctt tatggctgaa
      721 tgaatcgtta taaaaggtat gcgataactg ccatgacgtt tgatcgccat cctgttcgct
      781 ataacgataa tccgtagaca catttgttga atggctgttg ttattgctat ccatcgttgg
      841 cgtgtcagat aacgaaaacg ataagtacaa gccgttgtcg tcgttatcgc caccgttgtc
      901 attacgatgg aaaacaccga ctgacgtact caaaatccag tcacgcatca caaaagcgcg
      961 ggaagcgcta agttgtacag tttcagagat actttgcgct gttgtctggc gaagatagtt
     1021 attttcaaag ctctgctgtt tatcccaacg caaactatca tcagtgttat tttgtgtgcg
     1081 ggtataaccc agcatcatat tccagccgac aaaattctgt gaaaccgtcg cggaaatact
     1141 ttcataacag cttaaggcgt tatagctctc tctgctgtcg gttgtacaag catcgccgtc
     1201 agagttggtc cgataaaagc ccaacgtcgg ccatcccgga ttactccagt tggcctgttg
     1261 catatcacct ttgccgccgt catcgttacg gaacacgctg gcgctgattg caagattccc
     1321 caccccgcct aaatctgccg tccagttatt acctaactcg aaagcactca catcatcggc
     1381 attcgccagc cctgcgtaga gctcatattg cggatgtaat ggcaggcgta ctcctagctg
     1441 ataagctgaa ctttcatcat cagaaacctg tgatgtagtt ttacctgcct gtaagaacca
     1501 ttgcgcattt ccgtcagtca gaccgccggt tttggtaaac ggcacaagct cggtgcgggt
     1561 gagttggtta ttttcgtaga cttttaatgc cacgctgtag ctgcctggcg gaaaggaact
     1621 ggtatcaata aattgcgaac cactattgag ataaaacgat cccaaaagtt gctcattacg
     1681 ataagcgtca acacgagaat tgcgcgaaag cagaaccatt actggggttc cttgctggct
     1741 ttgcgcctgg tttaaatagc tcagtgtcga cccgatacgc atcccatcaa ttgcaccgag
     1801 tggcagaaag ttaaaggtaa agttcccgcc ttgtgcatta aatagtgtgc ggttgtccat
     1861 gcgacccgcc tgcacataat aacgacgtaa aaaatcataa cgataataga gatcgctgat
     1921 gtcagcatta ctgtcactga catcatctgc atcataaccg ttgaaattcc agtgtgcacc
     1981 aatataacta ttttctgtta ttcccagcgc accgtttccc tgaatagaca acgattggta
     2041 atcatcctgt gccagaacat tgatgtcttg ctgatgtata aaagcattaa cagtattttt
     2101 gtctggattt aaatataatg acttcgcatc gaaagctgaa ttccattgcg gattaataaa
     2161 caacgttgcc gaactttctt catcattata gattatttcc agcgtatcgg tattcagaaa
     2221 accacaccct gagtcagtac gtacataacc gcaagccaat tcaccgtggc gagctagcgg
     2281 ctgcgataat ttttctttca ctatatgcgc gattttctga tcgtctacat ccagttccag
     2341 cttgttatat agacttgctg gatcaagaaa tgttacggta tcaagattaa ccattgcata
     2401 atatttaccg agacttttcc ctgaaaaata aacctcgata tactcctgct gtccctgagc
     2461 aataacctca aaccctgcgg gaattttggt taatgtcgcc tctgccgggc tgagactcgc
     2521 caggatcaaa agagccagta attttttatc catggcaatc ctgaaatcca taacaaatac
     2581 taaaacagtt aaaataaggg agggctaccc ctccctttgt tacgtgttaa ttctgttatc
     2641 tggattagat atcctgagac agataaatgc ttactacacc acggtagatg cctgtctcca
     2701 gaacaccttt ggtggtttga gcaaacatca ggtttttagc cagagaaccg ttagttaacg
     2761 catcggaagc aaagattttg gttgcggtaa aagtggttgc cgcatcagct ttaatttctt
     2821 caccgcccca ggttactgtc agcggaacaa tcttgctagc atccaggctt tgaaccagtt
     2881 gcgccgggga aactaactgc atttttacat ctttggtaac gtcgttagac cagattttgg
     2941 tcatcaactg gtaagattgc agaccctgcc ccggcagata ttgcatttct actgctttcg
     3001 gcaacgcggt gttatcggtc tgtgtcatat ccagagcggc atcaacgttg gccgttacag
     3061 taatatcctt ttgtactgcc aatgcggagc ctgcaacaga aaacatcgct gcgaccagaa
     3121 gagattttgc aaataccttt ttcattacta ttccttcgtt tattacaatg ataattaatg
     3181 gaccgccagt tcaaactcgc cattatcttt ggtgatccag ttattatatt tgacaacgat
     3241 tttgtcgtag ccagatatgc tttcaagatt tctttcctga ccagggaaga tgtttttatt
     3301 tacggtctgt atcttacaat tttctttgct attattttta caataagcga tcgttaaaat
     3361 ctttaagcgt tgattccctg cattaactaa tttttgggca gcatttactt ctaacttagc
     3421 aataggttgc tgaggtggaa cactaacaac aatcccccaa attaagttaa ctgttaactg
     3481 catagagaga tcttttttat ctgtggcaac gtcatcaata ctgggaaccg cttcaaattt
     3541 aacgcgataa tttttctctt tttgctctgg ttccatcgca acaaaacgta cggttttgct
     3601 actaccggct ggtaaagcga atttaggtgg cataacaacc agatgatttg catcaccgga
     3661 tttaatctca acttcatttt cctgaggcgt cgatggatta tcgatacgga atactgtcgc
     3721 cttgatgtac tgaacttcat tacttttaga tattacgcga acgttgcctt caccctgact
     3781 attaatggaa acagccatag gataaacagt catatttgac tgtgccaaaa aaggagcgaa
     3841 cacgaataaa agcggcgtaa gccgtgataa gagattaatt ttcgccacca tataaatttg
     3901 atcttacaat attgtttcca aaaagttggt gtattagaga tgtagattta ttgtaagtca
     3961 atataaaaaa gcatttttag ttaatattaa atttaaaggc tgggaattaa cttactgaaa
     4021 tt
//
LOCUS       DAZUFB010000229         4015 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZUFB010000229 DAZUFB010000000
VERSION     DAZUFB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.230"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1593)
                     /locus_tag="ACHY3M_005078"
     CDS             complement(<1..1593)
                     /locus_tag="ACHY3M_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4319790.1"
                     /translation="MYLTDNIIKLSNKIGLRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     CNQLLKSLYYDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDN
                     TLITFSNEEIIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNR"
     gene            complement(1630..3498)
                     /locus_tag="ACHY3M_005079"
     CDS             complement(1630..3498)
                     /locus_tag="ACHY3M_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFS4319791.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIAPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIGGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            complement(3587..>4015)
                     /locus_tag="ACHY3M_005080"
     CDS             complement(3587..>4015)
                     /locus_tag="ACHY3M_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319792.1"
                     /translation="AECEIYLNTDFDLHFLKNEICEALNRHVRVIVFSFNKLITPDPR
                     VELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEHIHSD
                     IYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT         1236 a          783 c          743 g         1253 t
ORIGIN      
        1 tcgattatgg gctttagttg ctgactctga tgtaaagcac ccttgatgag cctcgaagta
       61 aagttcaccc gaaataatgg gcaaggattc gcgaaaggcg tcggcgtgag tgaagaactt
      121 atcaacacgg ctgaaatcaa catgaggtaa gccatcaata ttgcgtatgc gtgttaatcg
      181 ttctatatgt tcctcaccgg ggccagcgcc gccatcaccg acaccataca ccataagcgc
      241 atagggacag atttcttttt cattatagtt ttgttcaata tattctaaag agcgagcgcg
      301 ggctcggcta tcatacgtat cttcaggaaa gttatgcatc agaatttctg agccatccag
      361 gccttgccag cggaaaaggt aattattaaa tttattaatc ttgttttgtg agagcttttg
      421 cgatgcaata tagttaatgc ctgatttttt gagaatttgt ggaagcgtgg cggggaatcc
      481 aaagacatcc ggttgccaga cataatttat cttctttgaa aaattctgca gtgtgaatcg
      541 tgtcccatgc atgatttgcc ttatcagcga ttcaccactt accagattta aatcgcattc
      601 gacccaaaag caaccttgta actcaaagcg gcctgcggcg atttgttttt ttaacttttc
      661 gaagaaatag ggataatctt tttttatcca gtgaaaaagt tggtactgac tagcgccaaa
      721 aatgtaatca ggatattttt caatgtttgt cagggcggtg gcaaatgttc ttattgcttt
      781 tctgcgtcct tctcttaaag gccacatcca cgctatatct aaatgggcat gaccagtagc
      841 cataatcctg aactctgaag gattattgtt tttactaatt atggattgga ttgcaatttg
      901 acatttaatt atttcttcat tgctaaaggt aattaacgta ttatcaatat attcgagaat
      961 tcttatgatt tcgtcagtac gctcgcttct tttggtgata attttttctg ggataatacc
     1021 tttaggatgt atggattcaa acttctgacc atcattaatc cagtcgaaaa gcacttcaac
     1081 atcataataa agggatttca gtagttgatt acatgtcgcg atatgaacat cagtaataat
     1141 ccctccatta gtgacatagc caaataaatc gttgcacgca gcatcaatcc aaaactcgac
     1201 ctcgtcattt tcactaacga gagaaaggtc aaccacccat ttacctggtt tatctggcgg
     1261 tacgccataa gatgatgctt tattcgtgat gccgcagatg ggtgaaccat tcttatccac
     1321 aataagacct tcgccaccga tatcaacgag agcgacgagt ttgtgtctaa atttatctgc
     1381 tggacgttta ccataaactc gcatccaggc acagtcaaac agttgacccc agtctgagcc
     1441 aatggtgagt ttctggtaag cgccgctacg gcgataaaca aaaggctgcg gttcttttgt
     1501 tatccatgct tcaatcgaca tttcagagac aacagtataa acacgcagac ctattttatt
     1561 cgacagtttg ataatattat cagtaaggta catagaaatc cctttaatta attataatac
     1621 tcgaaaatat taactctcta tattggagaa agttactttg cgaagaatgg tttgaccaaa
     1681 tggtataagt tttctgacta ttttagtctt gtttaaataa aaacagcctc caatgtattg
     1741 tttgccctct ggtgaggttg aatatgaaaa agattccata tcagcataag agaactggat
     1801 agattcatca gctctactac agggtatgta tcctgagtca aaatatttcc cgttcagcag
     1861 agtttttttg attaaaatat cgctctcaag ctcaattgca tataaaacgg gacctctgga
     1921 aatataagtg tcaccacata agtcagtatt aaactgtata tcagctataa atttaatgtc
     1981 aatcacatca tgatgttgcc atgttctatc taaaataata agtgagtttt gagtatcatt
     2041 tttagtaaaa gtaacaccat taataatcat catttttgcc cagccggggt aacggaaact
     2101 gagagcaaaa cgaacagatg actcaggctc aatgagacat tttacgctta aatcatgagg
     2161 gtattcagtt aattgtgtaa ttttaattgc aacattatta aatgtgccat aaaactcaca
     2221 cgggccataa aataatgcag taaaaccatt gttattttct tgaaacatat tttcgacaaa
     2281 agccggtatg gttcttgctg tattcggtac gcagcagaca gcagcctcct catgagtagg
     2341 ggagtattta tatctggtat taaactgatt actgtccgga tgtttccttt catttgcttc
     2401 ataacaatta tcagtcttac agtacattat cgcgtgaagt atgggatgct gcatgccaaa
     2461 actagcatta tagaatagcc actcagcatc atcggccaga ccaatattag tggtcagttt
     2521 tattaacata acataagtgc taaatagttc atgaacagaa caatattcat agcctgtata
     2581 agtggcatta gctgtgcgtc ctgtaatcca ctcatcacca atgggagcgc cagatggtgt
     2641 aagataatat ttcaactggg ataaaaaaat atctaatgca gtttgatact gcggccgttg
     2701 gctcgctgcg atggctaatg ccctaatatg ctcatacgta tgtacgccat gacctttaag
     2761 aaggtaacca ggggctatga catttttata ttgcagatct tgctccgaca cgcatcctgc
     2821 ggaataatct tcatacagcc acaagcaata tgagagatat tcaatgttat tggttatttc
     2881 ataaaggcgg tagaaaacat ctgttattgt taatccgtgg ctatgtccag caaaaccctg
     2941 ttccacaacg aaaggatgtg acgtttcttt agggtagcca gccataatgc attgtgcggc
     3001 ttgggtaaga gaagttaata aactctcatt acgggtggac tcataacatc caagcacaaa
     3061 acgcaaggca gtagcttgtg cccagaactc tccattttct gctttacagt taaattttaa
     3121 tgatgaatca taaattccaa tatagccatc atcatcttgc gtatcgataa gttccaaaca
     3181 taaatcgctc gctttttcct gccattcttt attatctaac aagtaggcac tgcggcaata
     3241 tccatccttc caattcgatt gagtttcact atcccaccaa taatattgca tcatgttatc
     3301 atcgggatta ttctgttttt taataacgcc atcttcagca gatacagtgc gtggagttat
     3361 acgatctctt ccatatattt tatgctccgt aataagatgg ggaactaatt tatccagttc
     3421 tcctatatga ccgttcatat accattccat cttattcttt aaccaaccag tgggctttat
     3481 atcgttaaaa aaaatcataa atataccttt gcataaaaaa gtttagacgt tataaaagac
     3541 aaatagattg tgatcgccta cagacgatca caatgaaagg aaaatattaa cggtttttgc
     3601 tatcactcaa atgtttttca tcctgcacca tatcattgcc agattcatgc aaagtattta
     3661 ttaatgcatt ggcatctgga tattgttgct gatacttggt tagatatata tcactatgga
     3721 tatgttcggc aattatattg ataagcaagc gattattcga gtataacccc agtgattcag
     3781 ctcgttgtga aaaaaccatt actgtcttca tatcaacaac aagcataaaa cggtgagaag
     3841 ggtaatattc ttcttcatgg ttaaagcggt aatacagttc tacacgtggg tcgggagtaa
     3901 taagtttatt aaatgaaaaa acaataactc gaacatgtcg attcagagcc tcacaaattt
     3961 catttttcaa aaaatgtaag tcaaaatccg tgttgaggta aatttcacac tctgc
//
LOCUS       DAZUFB010000230         3860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZUFB010000230 DAZUFB010000000
VERSION     DAZUFB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.234"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..366
                     /gene="cesT"
                     /locus_tag="ACHY3M_005081"
     CDS             <1..366
                     /gene="cesT"
                     /locus_tag="ACHY3M_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFS4319793.1"
                     /translation="IYYISLSDASDEYMMIYGVCGKFPTDNPNFALEILNANLWFAEN
                     GGPYLCYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENE
                     HMKIEEISSSDNKHYYAGR"
     gene            424..3231
                     /gene="eae"
                     /locus_tag="ACHY3M_005082"
     CDS             424..3231
                     /gene="eae"
                     /locus_tag="ACHY3M_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFS4319794.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNVAQDRLFYTLKTGETVSSISKSQGISLSVIWSLNKHLYSSESEML
                     KAAPGQQIILPLKKLSVEYGALPVLGSAPVVAAGGVAGHTNKMTKMSPDATQSNMTDD
                     KALNYTAQQAASLGSQLQSRSLHGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQLRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADGTEAITYTATVKKNGVTQANVPVSFNIVSGTATLGANSATTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFVEQTKASITEIKADKTTAVANGNDAVTYTV
                     KVMKEGQPVQGHSVAFTTNFGMFNGKSQTQNATTGSDGRATITLTSSSAGKATVSATV
                     SGGNDVKAPEVTFFDGLKIDNKVDILGKNVTGDLPNIWLQYGQFKLKVSGGNGTYSWH
                     SENTNIATVDESGKVTLKGKGTAVINVTSGDKQTVSYTIKAPNYMIRVGNKASYANAM
                     SFCGNLLPSSQTVLSNVYNSWGPANGYDHYRSMQSITAWITQTEADKISGVSTTYDLI
                     AQNPHKDVTLNAPNVYAVCVE"
     gene            complement(3520..>3860)
                     /gene="escD"
                     /locus_tag="ACHY3M_005083"
     CDS             complement(3520..>3860)
                     /gene="escD"
                     /locus_tag="ACHY3M_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFS4319795.1"
                     /translation="NIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQNIQ
                     PYISADIFPGKILRISGTMTNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISRP
                     DEYIHIPLSY"
BASE COUNT         1142 a          742 c          856 g         1120 t
ORIGIN      
        1 atttattata tttcgttatc tgatgccagt gacgaatata tgatgattta tggcgtctgt
       61 gggaaattcc cgacagataa ccctaacttc gctcttgaga ttttgaatgc aaacttatgg
      121 tttgcagaga atggtgggcc atacctgtgt tacgagtcag gagcacaatc gttgttgtta
      181 gcgttacgtt tcccgctcga tgatgctacc cctgaaaaac ttgagaatga aatagaagtc
      241 gttgttaagt caatggaaaa cctgtatttg gtattacata atcagggaat aacattagaa
      301 aacgaacata tgaagataga ggaaattagc tcaagcgata ataaacatta ttatgccgga
      361 agataaaatc tatttattaa tctaatttac tactcattct aactcattgt ggggacccca
      421 aacatgatta ctcatggttt ttatgcccgg acccggcaca agcataagct aaaaaaaaca
      481 tttattatgc ttagcgctgg tttaggattg tttttttatg ttaaccagaa ctcatttgca
      541 aacggtgaaa attattttaa attgagttca gattcaaaac tgttaactca aaatgttgct
      601 caggatcgcc ttttttatac gttgaaaaca ggtgaaactg tttccagtat ttctaaatca
      661 caaggtatca gtttatccgt aatttggtca ctgaataaac atttatacag ttccgaaagc
      721 gaaatgctga aggctgcgcc tggccagcag atcattttgc cactcaaaaa actgtctgtt
      781 gaatatggtg ccttacctgt cttaggttcg gcacctgttg ttgctgcagg tggtgtcgct
      841 gggcatacaa ataaaatgac taaaatgtcc ccggacgcga ctcaaagcaa catgactgat
      901 gacaaggctc taaattatac ggcacaacag gccgcgagcc ttggtagcca gcttcagtcg
      961 cgctctctgc acggcgatta cgcgaaagat accgctcttg gtatcgcggg taaccaggct
     1021 tcgtcacagt tgcaggcctg gttacaacat tatggaacgg cagaggttaa tctgcagagt
     1081 ggtaataact ttgacggtag ttcactggat ttcttattac cgttctatga ttccgaaaaa
     1141 atgctggcat ttggtcaggt cggagcgcgt tacattgact cccgctttac ggcaaattta
     1201 ggtgcgggtc agcgtttttt ccttcctgaa aacatgttgg gctataacgt cttcattgat
     1261 caggattttt ctggtgataa tacccgttta ggtattggtg gcgaatactg gcgagactat
     1321 ttcaaaagta gcgttaacgg ctatttccgc atgagcggct ggcatgagtc atacaataag
     1381 aaagactatg atgagcgccc agcaaatggc ttcgatatcc gctttaatgg ctatctacca
     1441 tcatacccgg cattaggcgc caagctgatg tatgagcagt attatggtga taatgttgct
     1501 ttgtttaatt ccgataagct gcagtcgaat cctggtgcgg cgaccgttgg tgtaaactat
     1561 actccgattc ctctggtgac gatggggatc gattaccgtc atggtacggg taatgaaaat
     1621 gatcttcttt actcaatgca gcttcgttat cagtttgata aaccgtggtc tcagcaaatt
     1681 gagccacagt atgttaacga gttaagaaca ttatcaggca gccgttacga tctggttcag
     1741 cgtaataaca atattattct ggagtacaag aagcaggata ttctttctct gaatattccg
     1801 catgatatta atggtactga acacagtacg cagaagattc aattgatcgt taagagcaaa
     1861 tacggtctgg atcgtatcgt ctgggatgat agtgcattac gtagccaggg cggtcagatt
     1921 cagcatagcg gaagccaaag cgcacaagat taccaggcta ttttgccggc ttatgtgcaa
     1981 ggcggtagca atatttataa agtgacggct cgtgcctatg accgtaatgg caatagctct
     2041 aacaatgtac agctcactat taccgttctg tcgaatggtc aggtggtcga ccaggttggg
     2101 gtaacggact ttacggctga taagacttcg gctaaagcgg atggcaccga ggcgattact
     2161 tataccgcga cggtgaaaaa gaatggggta actcaggcta atgtccctgt ttcatttaat
     2221 attgtttcag gaactgcaac tcttggggca aatagtgcca caacggatgc taacggtaag
     2281 gcaactgtaa cgttgaagtc gagtacgcca gggcaggtag tcgtgtctgc taaaaccgcg
     2341 gagatgactt cagcacttaa tgccagtgcg gttatatttg ttgagcaaac caaggccagt
     2401 attactgaga ttaaggctga taagacaact gcagtagcaa atggtaatga tgctgttaca
     2461 tacactgtta aagtgatgaa agagggtcag ccagtgcagg gacactccgt tgcattcaca
     2521 acaaactttg ggatgttcaa cggtaagtct cagacgcaaa atgcgaccac gggaagtgat
     2581 ggtcgtgcga cgataacact gacttccagt tccgcaggta aagcgactgt tagtgcgact
     2641 gttagtggtg ggaatgatgt taaagcacct gaggttacat tttttgatgg actgaaaatt
     2701 gacaacaagg ttgatattct tggtaagaac gttactggtg acttacctaa tatctggttg
     2761 caatatggtc agtttaaact gaaggtaagc ggtggtaatg gtacatattc atggcattca
     2821 gagaatacca atattgcgac tgttgatgaa tcagggaaag taaccttgaa aggaaaaggt
     2881 actgcagtaa ttaatgttac atctggtgat aagcaaacag taagctacac tattaaagct
     2941 ccgaattata tgataagagt gggtaataaa gccagttatg caaatgctat gtccttttgt
     3001 ggaaatttat taccatcctc acagacggta ttatcaaacg tttataattc atgggggcct
     3061 gcaaacggat atgaccatta tcgttctatg cagtcaataa cagcttggat tacacaaact
     3121 gaagctgata aaatatcagg agtatcaact acttatgact taatagcaca aaaccctcat
     3181 aaggatgtta cgctaaacgc tccaaatgtc tatgcagttt gtgtagaata atttaaaatc
     3241 ctttccccgg ctaaaaatat attattttag ctggggaatt attgttgttt ctttattatc
     3301 ttcttcttaa taagtaacgt cctagaatca acatctcctg ttggttaata agttaatctc
     3361 aaagaaacat attcacgttt cctcatgtag cgactcgact actttcaacg agccaacctc
     3421 gctaatacca aagctgaatc acatcaagca acaactatgc tcacaacatc catacaataa
     3481 ataaaaaaac cctccgaaga gggggaagat gacaagacgt taatacgata gcggaatatg
     3541 tatatattcg tctgggcggg agatgttgat accatcttta ggatcaatcg cgtctatgac
     3601 atagcctcct ttcagaatgc taccaattcc caggcttgta ccgttatcaa gagcaattgt
     3661 tggattcgtc atcgtgccgc tgattctgag tatttttcca ggaaagatat ctgctgaaat
     3721 gtacggttga atattctgaa atagtatctt tacgttacta tttttgttca ttgcatttat
     3781 aatggcaagg atcttactct cgttctgttg agaaacttcc cccgcaacaa tgatgacatc
     3841 attctttttg aaaatattga
//
LOCUS       DAZUFB010000231         3854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZUFB010000231 DAZUFB010000000
VERSION     DAZUFB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.235"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHY3M_005084"
     CDS             <1..249
                     /locus_tag="ACHY3M_005084"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFS4319796.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGRGVRRAA"
     gene            246..428
                     /locus_tag="ACHY3M_005085"
     CDS             246..428
                     /locus_tag="ACHY3M_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS4319797.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            425..595
                     /locus_tag="ACHY3M_005086"
     CDS             425..595
                     /locus_tag="ACHY3M_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFS4319798.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCSDCCAELMSD
                     PNSSMYEEEDDE"
     gene            588..806
                     /locus_tag="ACHY3M_005087"
     CDS             588..806
                     /locus_tag="ACHY3M_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319799.1"
                     /translation="MSDSFSSSDYLYLVLVPVAEVFRSRFPEGSAPFNAISTYSKCRV
                     KFTGKRLEREWQQFCKKHDLKNDPELEY"
     gene            807..1097
                     /locus_tag="ACHY3M_005088"
     CDS             807..1097
                     /locus_tag="ACHY3M_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS4319800.1"
                     /translation="MANLRKEARGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDTRLYHLEGVIRTQAILLKEGKIKS"
     gene            1094..1456
                     /locus_tag="ACHY3M_005089"
     CDS             1094..1456
                     /locus_tag="ACHY3M_005089"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319801.1"
                     /translation="MNEYQFVLPYPPSLNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            1453..1641
                     /locus_tag="ACHY3M_005090"
     CDS             1453..1641
                     /locus_tag="ACHY3M_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFS4319802.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            1638..2261
                     /locus_tag="ACHY3M_005091"
     CDS             1638..2261
                     /locus_tag="ACHY3M_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS4319803.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDISKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            2696..3019
                     /locus_tag="ACHY3M_005092"
     CDS             2696..3019
                     /locus_tag="ACHY3M_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFS4319804.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3003..3479
                     /locus_tag="ACHY3M_005093"
     CDS             3003..3479
                     /locus_tag="ACHY3M_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFS4319805.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLISKFKEAGGTVREIEV"
     gene            3566..3793
                     /locus_tag="ACHY3M_005094"
                     /pseudo
     CDS             3566..3793
                     /locus_tag="ACHY3M_005094"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            3786..>3854
                     /locus_tag="ACHY3M_005095"
     CDS             3786..>3854
                     /locus_tag="ACHY3M_005095"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4319806.1"
                     /translation="MEKAQDLQQMLNSIITVSEVEST"
BASE COUNT         1119 a          903 c          982 g          850 t
ORIGIN      
        1 atgcttgtgc cagaggatat gtcagtcgga tggttcagca aggctctgga gagtgtcgac
       61 gaagttcgca ttatcactga tggacggatt aattttatcg aaccatcgac agggctggag
      121 aagaagggaa acagtaaagg ctccatgctg ctgatttggc gaccgttcat cagtcctcga
      181 cggatgttta ctaccgtatc caaagcggca ttgatggcga tcgggcgggg cgtcaggagg
      241 gcggcatgag acgacagcga cgaagtatca ccgacatcat ctgcgaaaac tgcaaatacc
      301 ttccaacgaa gcgatccaga aataaacgca agccaatccc aaaagaatct gacgtaaaaa
      361 ccttcaacta cacggctcac ctgtgggata tccggtggct aagacatcgt gcgaggaata
      421 caaggtgatt gacccaaatc gaagttacga acaagaaagc gtcgagcgag ctttaacgtg
      481 cgctaactgc ggtcagaagc tgcatgtgct ggaagttcac gtgtgctccg attgctgcgc
      541 agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg agtgattcgt
      601 ttagtagctc tgattatttg tatttggttt tagtacctgt ggcagaagta ttccgctctc
      661 gatttcctga aggctcagca ccatttaatg ccattagcac ttactcaaaa tgcagagtga
      721 aatttacagg taagaggctg gaaagagagt ggcagcaatt ctgcaaaaaa catgacctaa
      781 aaaatgaccc tgaactggag tattaaatgg ctaatctacg caaagaagcg cgcggcagag
      841 aatgccaggt acgtatttac ggcatatgca atggcaatcc tgaaactaca gttctggcac
      901 attaccggat ggctggaatt tgcggaacgg gaatgaagcc tgacgacctg atcggcgcat
      961 gggcttgtag cgcgtgtcac gatgaaatcg accgacgcac ccataacctc gacaacaaag
     1021 acaccagact ttaccacctc gaaggcgtta tcaggacgca ggcgatactg ctgaaggagg
     1081 ggaagattaa gtcatgaacg aatatcagtt tgtgcttcca tacccgccgt cgctgaacac
     1141 ctactggcga agacggggaa gccaatacta catcagcgat aaaggccaga aataccgaaa
     1201 agacgttcag caaatcatcc gccaacttaa gttagacatt ttcaccaaat cacgactccg
     1261 catcaaagtc atcgcagacg ttccagactc ccgccgccgc gacctcgaca acatcctgaa
     1321 aggtttactc gactccctta tccacgccgg atttgcggaa gacgacgagc aattcgatga
     1381 cattcgcgta attcgtggtg tgaaagtacc aggcggacgg cttggaataa aaatcaccga
     1441 actggagaac gtatgaacgc cacaattcaa acgataccag agcttcttat acagacacga
     1501 ggcaatcaga ccgaagtggc gaggatgctt tcctgcgcaa gaggaacggt gctcaagtac
     1561 aaccgagaca gcaaaggcga acgtcacgta atagttaacg gcgtcctgat ggtcaaacag
     1621 ggcaagagag gaagacgatg agactcgaaa gcgtagctaa atttcattcg ccaaaaagcc
     1681 cgatgatgag cgactcacca cgggctacgg cttctgactc tctttccggt actgatgtga
     1741 tggctgctat ggggatggcg caatcacaag ccggattcgg aatggctgca ttctgtggta
     1801 agcacgaact cagccagaat gacaaacaaa aggctatcaa ctatctgatg caatttgcac
     1861 acaaggtatc ggggaaatac cgtggtgtgg caaagcttga aggaaatact aaggcaaagg
     1921 tactgcaagt gctcgcaaca ttcgcttatg cggattattg ccgtagtgcc gcgacgccgg
     1981 gcgcaagatg cagagattgc cacggtacag gccgtgcggt tgatatatca aaaacagagc
     2041 tgtgggggag agttgtcgag aaagagtgcg gaagatgcaa aggcgtcggc tattcaagga
     2101 tgccagcaag cgcagcatat cgcgctgtga cgatgctaat cccaaacctt acccaaccca
     2161 cctggtcacg cactgttaag ccgctgtatg acgctctggt ggtgcaatgc cacaaagaag
     2221 agtcaatcgc agacaacatt ttgaatgcgg tcacacgtta gcagcatgat tgccacggat
     2281 ggcaacaaat taacggcata atattgactt tttgaataaa gttgggtaaa tttgacatca
     2341 acgatggata aatgcactcg ttaaataaag ccctgagtta atagctcggg gctttttgcg
     2401 ttttaatcac gacctttctg aaagcacatc aaaccaaata ccagacagac aaaaataatc
     2461 accttatccg ctgtggctac ggtgcggtgt gctttgcata aaagaaaacc agcgcaatgg
     2521 ctggcttcgt gaaagcgggt ggcaagaggt tgcgctaaca acctcctgcc gttttgcccg
     2581 tgcatatcgg tcacgaacaa atctgattac taaacacagt agcctggatt tgttctatca
     2641 gtaatcgacc ttattcctaa ttaaatagag caaatcccct tattgggggt aagacatgaa
     2701 gatgccagaa aaacatgacc tgttagccgc cattctcgcg gcaaaggaac aaggcatcgg
     2761 ggcaatcctt gcgtttgcaa tggcgtacct tcgcggcaga tataatggcg gtgcgtttac
     2821 aaaaacagta atcgacgcaa cgatgtgtgc cattatcgcc tggttcattc gtgaccttct
     2881 cgacttcgcc ggactaagta gcaatctcgc ttatataacg agcgtgttca ttggctacat
     2941 cggtactgac tcgattggtt cgcttatcaa acgcttcgct gctaaaaaag ccggagtaga
     3001 agatggtgga aatcaataat caacgtaagg cgttcctcga tatgctggcg tggtcagagg
     3061 ggactgataa cggacgtcag aaaaccagaa atcatggtta tgacgtcatt gtaggcggag
     3121 agctattcac tgattactca gatcaccctc gcaaacttgt cacgctaaac cccaaactca
     3181 aatcaacagc tgcaggccgt taccagcttc tttcccgttg gtgggatgcc tatcgtaagc
     3241 agcttggcct gaaagatttc tctccgaaaa gccaggacgc tgtggcattg caacagatta
     3301 aagagcgtgg cgctttgccg atgattgatc gcggtgatat ccgtcaggca atcgaccgtt
     3361 gcagcaatat ctgggcttca ttgccgggag ctggttatgg tcagttcgag cataaggctg
     3421 acagtctgat ttcaaaattc aaagaagcag gcggaacggt cagagagatt gaggtatgag
     3481 cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc tgtcatgggc
     3541 tgttaatcat taccgtgata acgccatgac ctacaaagag cagcgcgata aagccatatc
     3601 catcatcgct gatatgcaga agcgtcaacg tgatgtagca gaacttgacg ccagatacac
     3661 aaaggagctt gctgatgcta acgcgactat cgaaagtctc cgtgctgatg tttctgctgg
     3721 tcgtaagcgc ctgcaagtcg ccgccacctg tgcaaagtca acgaccggag ccagcagcat
     3781 gggcgatgga gaaagcccaa gacttacagc agatgctgaa ctcaattatt accgtctccg
     3841 aagtggaatc gaca
//
LOCUS       DAZUFB010000232         3791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZUFB010000232 DAZUFB010000000
VERSION     DAZUFB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.236"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..360
                     /locus_tag="ACHY3M_005096"
     CDS             <1..360
                     /locus_tag="ACHY3M_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFS4319807.1"
                     /translation="IFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKSSPRD
                     ELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLMTLLS
                     DAMFAAEMKGMLDGTGD"
     gene            344..1675
                     /locus_tag="ACHY3M_005097"
     CDS             344..1675
                     /locus_tag="ACHY3M_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFS4319808.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKAARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDAPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            1797..2270
                     /locus_tag="ACHY3M_005098"
     CDS             1797..2270
                     /locus_tag="ACHY3M_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS4319809.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            2447..3571
                     /locus_tag="ACHY3M_005099"
     CDS             2447..3571
                     /locus_tag="ACHY3M_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFS4319810.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAARLLGKTEAEFLKMKEEMQ"
     gene            3571..>3791
                     /locus_tag="ACHY3M_005100"
     CDS             3571..>3791
                     /locus_tag="ACHY3M_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFS4319811.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYG"
BASE COUNT          894 a         1020 c         1117 g          760 t
ORIGIN      
        1 atcttccgtg tccgtcagag tggcaatgaa cctgctcaga cagacaaaga gatgccaccg
       61 gaaaaacagg agaagacaga gcagacagcg ctggctgcca gactgccgga agcgaagagc
      121 agcccccgag atgaactgga cgacatgggg gacgcggtgc ctgcccgccg gttacaggag
      181 gccatcgacc cggtactgga gccggtcatt gaagccatca gaacgcgggg gctggcggag
      241 gcactggcag acctgcctgc cctttaccgt gaaatggatg attcccgcct gatgacgctg
      301 ctcagtgatg ccatgtttgc tgcggaaatg aaggggatgc tggatggcac aggggattga
      361 tttaggttat gccgccacgc ttccctcaaa agaggcggtg gcatacttcc gcgccaaagg
      421 ggcgcatatc agctggaact ggttcgaaac agacgcggat gttcatgccc gttcattcac
      481 ggcggcaaaa gcggcacgcc tggacgtact gaccacacta caggcggaag tgcagcgtgc
      541 tgttgatgag gggatttcac agaaagcatt tatccgcaca ctgacacccc gcctgcaaaa
      601 gctgggatgg tggggaaagc agattgtggt ggacagcgcc ggtaacgcag aagaagtgca
      661 actgggcagt ccccgccgtc tggcgctgat ttacaacgtg aacacccgcg tggcttacaa
      721 tgccgggcgt tacacgcaga tgatgaacaa cacggacacg catccgttct ggcagtatgt
      781 ggcggtgatg gacagccgca cccgcccggc gcattccgcc cttaacgggc tggtattccg
      841 ctatgacgct ccgttctgga atacacacta cccacccaat ggctggaact gccgctgccg
      901 tgtccggcca ttgtctcagg cccgtctgga tgcgatgggg ctgtctgttt catccggtca
      961 ggatcatctt tccacccgca atgttgaggc tggcgtggat aagcagaccg gagaagtcag
     1021 agaaatgccg gtgaccacat attcagatgg caccagaacc atgacaccgg atgtgggctg
     1081 gtcatataac cccggctcgg cggcgttcgg cacagaccag gcgttgatcc gtaaactgat
     1141 cgaggtgaaa agcccggcgc tgcgggaaat ggtggtgcag gagatgaaca acagcccgga
     1201 gcggcagctg gcgttccgca tctgggcaaa aaacatcatg aaaacccggc gaggcggtaa
     1261 cgatatccgc acgctgggct ttatgaccga aagtattgcg caggcggtgg aaagccggac
     1321 gggaacgcca ccggcccgcc tgctggcgat gagcggtaaa aatgtgctcc atgcggacag
     1381 catgaaacat cagaatgacg gtatcgccct gacgccggag gatttcggtc gattaccggc
     1441 aatgctggca aagccaaaag cggtgctgtg ggacaaacgc cacaacaatc tgatgtatat
     1501 cgtggaaagt aaggatagca gtgtgcagat tgccgttaat gcgccgtaca gcctgaagcg
     1561 tcagccggat aaactggatg tgattgtgaa tgcttaccgg gttatcaata tggataagct
     1621 gaaatcggat atccggggtg gaatgcttga ggtactggaa ggagatattg attaacggtc
     1681 agtggcgggg gttgaacccg catacatgat ccacccggag gcaggcaccg actttaccag
     1741 ttaagcgtac actgaccgcc tgtttatttt atattcatcc tttatgcgga ggcaatatgt
     1801 catccattga tgccgctgtc gttgttgacg ttacgcgtct ccagcgggtc tttgcccggc
     1861 ttcagtttgt gggtggaggg aaagacctgg cccgcagtgt ggcggcgagt cttctgtcat
     1921 cgtcagaaat ggcgtttgaa caggaaaaag agccggacgg cgaacgctgg catgactggt
     1981 cagatcctta ccgcaagtgg cgtacccgta agggatacat gcccggcaaa atcctgacgc
     2041 tgaacggcga tctggcccga cgcctgacca cggattatgg cgatacctgg gcgctgattg
     2101 gatcaaatga gccttatgcg gccattcatc agtggggtgg cctgccaggt atgccgcctg
     2161 gaccggcggc cattggtgca cgtccgtata tgggctttga tcaggtggca gagcaggaga
     2221 tcatggacga aatcagaaaa cgctttaaaa aggccacaga aacgccttaa ttgtttaagg
     2281 tatgcaaatg cattacctta cccccttcag gcgcgtgtcg tgattttcta acctgtattt
     2341 aacgggcttt aaaatctgcg tgaagcgcct ttgtattttt cccctggaag cacttcctga
     2401 aatcccgcaa aacccgccct gaaaattccc ggcttatgct gccggaatga agacgaaaaa
     2461 cacgcccaaa cttgcttatg ccattctgaa tgccatcagc ctgtccgcag acggggacgg
     2521 tgactggtgt cagatcatgc cagccggtcg ggtaaaggcc cgtgacggtc gcccggaaaa
     2581 accggccgag ggctggctga tcaaccatgc agccgttgag cgcatggtct cccgggttgt
     2641 ggcgctcaat cagccggtga aaattgacta caaccaccag accctgatta aggggcatca
     2701 ggcaccggct gccggttttg tgatggcctc accggagaat tttcgcttca gtgaagagcg
     2761 tggcttcgag gtgcgcccga agtggaaccc tccggccctt gaacatctgc gtaataacga
     2821 attcccctgg ttttcaccgg tgattggcta tgacgagaac accggcgaac ctgtcgaact
     2881 tcggatgctg gctattaccg gtgaccccgg tctgaccggc atgaatcctg tcgccgcgct
     2941 gtcggcggat gacctttata acgccttaaa ccctccttta aaggatacct ccatgaatga
     3001 gcaattacgc cagttgctga cggcgctcgg tctgaccgtg gctgacggtg acgaatttac
     3061 gccggagctg ggcacggcgg cgctgtctgc cctcaccggg atcaaaaccc gtgcggatgc
     3121 acacgacaac ctgaaaacac aggtcgccag tttgtcggca gagctggaaa ccgcaaaagg
     3181 cacaccaact ggcggcacta ttgatctgac gaaatatgtg cccgttgaga cgtataacgc
     3241 cctgcgtacc gaatatgtcg cgctgtcggc acagcacggc agcaccacgc tggagcagtt
     3301 gctggataag gccgagtccg aaggacgcat tttcaaaagc gaacgcggat acatggaagg
     3361 actcggacaa cagattggtg ttgcggcact ttctgcacag cttgacgccc gtcagccggt
     3421 tgcggctctg actactcttc agaccgacac cgtaaccgtg ccggataaaa agaccgccac
     3481 cgctgcgctg tcggctgaag atatcgccgc cgcccgtctg ctgggtaaaa ccgaagccga
     3541 gttcctgaaa atgaaagagg aaatgcaata atgcctaccc cgattacacc ggcgatgatc
     3601 accgccctga tgacgggcta ccgttctgat tttcaggccg ggatgtccat ggctccgtcg
     3661 cagtaccaga aaattgcgat gaccgtgccg tccacgtcga aatccaacac ctacggctgg
     3721 ctggggcagt tcccgcagtt ccgtgagtgg atcggctccc gcgtcatcga gaagatgaaa
     3781 gcctatggct a
//
LOCUS       DAZUFB010000233         3735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZUFB010000233 DAZUFB010000000
VERSION     DAZUFB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.237"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(806..1408)
                     /locus_tag="ACHY3M_005101"
     CDS             complement(806..1408)
                     /locus_tag="ACHY3M_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFS4319812.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            1762..2001
                     /locus_tag="ACHY3M_005102"
                     /pseudo
     CDS             1762..2001
                     /locus_tag="ACHY3M_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2036..>3735)
                     /locus_tag="ACHY3M_005103"
     CDS             complement(2036..>3735)
                     /locus_tag="ACHY3M_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFS4319813.1"
                     /translation="DNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKN
                     SLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEA
                     QLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLL
                     EVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWL
                     QAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPK
                     YGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGF
                     TDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDE
                     ILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRR
                     RVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLE
                     RAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          829 a          910 c         1042 g          954 t
ORIGIN      
        1 catttcttac tttatgtcat ccatatctgt ggctatcatg tcattcgttt tatatgtcat
       61 aatcagaaga agaataggaa aagatttctt ttcagaaata aaaatgaaaa ggttaaaaaa
      121 agaagatatc atggggttag agcacgtaat ataggggcga ctggttctta taagggaccg
      181 tctagttatc gcggtagggt gtactcccgc gctatacggt gttttcagac cgcccgttga
      241 agcaggaacc agacgctgag caatcagccg tcacccacta atgggaatcc ccctcctgag
      301 tcacgaagtg cgaaggtggg ggaggatgtc aagtgtgcag tatcaaaaaa ctgtatcact
      361 ggtataatcc agtaaagagc atattgagaa cttattatgt cgtcacgcaa ctctgtacat
      421 gattttataa tgctgctccc ggtgctactg ttggtcgggt gggtagtttt tgtgataatt
      481 ctcatgtttc gggatgataa atgtgacaaa acttaccgtg aaggttcgtt tcccgaattt
      541 atttgtctcc attctgatcg ctcgtcactt cgagaaaccg attctgagaa actcattgtc
      601 aactatacag aaatacaggt atacaatcag gagaataggt aaaatccata atactatagc
      661 taaaaaaata tatacgggga tcaggcgtct gtatgcccgt tcatgtttat tcaggatgaa
      721 taaaatcttc tccgcttctg aatcacacat aacattctct tcccattttt tctgatacgt
      781 aatgtccacc tgttatcagg agtgtttatt gcccagaaat attctcctgc tgtgcctgca
      841 tcctgagcca cttttgttct aatgtcgcca gtctgcgacc ggcactctgt gcttcagagt
      901 ctgagacctg ccctttgggg tgaccatcca ggccataacg tgggacgccg gcgacagctg
      961 tccgctgcag atatacgcgg cgggatgtga tgcaggacac tgcacagagc cattcgtcca
     1021 tggtcagttc tgtctccggg tgttctgcca gaaatgctga aatatcattg tttatgccaa
     1081 ttttcagggg aataaatcca ccaccttcag cacgaagatg aggaaacagg gcctttattt
     1141 tttccacgca ctcgtgagta tactcacggg tccagtggga gggacgtgga aaacggtgtc
     1201 ttttcttttt cttcttcctg gctggcgctt cggggatatg tgtttcgacg gcagatgaat
     1261 ttattccggc gggggctgat tctgccgtct gttcagtaaa tacaggtgcg ataatatctg
     1321 ttttttcgga taaggacggt ggcgaaaaag tacgacgttt tttcaccaca atgacgggag
     1381 ttttcctttc ttcctgattt tttcccataa atacctcggc tggctggttt tactctttaa
     1441 taatatcaca attcataaat tagtaaattt cccatcaggt gatttttcat ggctaataag
     1501 tccgttctct gagtatttat actcgaggta gtaacagtta tggcgtgaca gtcgaattga
     1561 tattctactg aaccctgaca gaaacatggt tgatatattc tctcacttta atgttgtcat
     1621 gatataaata aaaacaattc atttgaaagg atatcagaag aagatatcgg ggcgcacagt
     1681 tcatctctgc ctttgtttca tggggtgttc tttgtatatt ttacttactg tttaagcatg
     1741 tacataagga gtgctttcat tatgccaatg atacttgtaa agaagaatcc ccggggcaaa
     1801 gtgattcggg agcttagcag tgaggaagag actgcagtaa agacggtctg tggactgaaa
     1861 aagccagcaa ccatggcact acataacctg gccaatgata ttctccggga aatgcgtgag
     1921 tatgatgcat ggttacagtg tgactgtatt ccgggagatt cgccggccat gaattttgct
     1981 gctcttaaaa acaatacggg taggggtcgt ctcagaaaac ggaaaataaa gcacgctaag
     2041 ccggttgcag aggccgtagc ggcctgaact tccccgcgcc gatcttggcg ctgctgcgcc
     2101 ataggtaatc accggtcagg ttgatgtgct cccagccgag tggcgacagg tactgcaata
     2161 gcgagtcatc gacggcatga ccattgccgc gcaacgcatg cgccgcacgc tccaggtaga
     2221 ccgtgttcca cagcacgatg gccgccgtca ccaggttgag gccgctggcc cggtagcgct
     2281 gctgctcgaa actgcggtca cggatttcac caaggcggtt gaagaacacg gcacgggcca
     2341 gcgcattgcg cgcctcgccc ttgttcagcc cggcatgcac gcggcggcgt agctcgacgc
     2401 tttgcagcca gtcgaggatg aacagcgtgc gctcgatgcg gcccagctcg cgcagcgcga
     2461 cggccaagcc gttctggcgc gggtagctgc cgagtttcct gagcatcagc gaggccgtca
     2521 ccgtgccctg cttgatcgag gtggccagcc gcaggatttc gtcccaatgg gcgcggacgt
     2581 gcttgatgtt gagcgtgccg ccgatcatcg gcttgagcgc gtcataggcg gcatcgccct
     2641 tcgggatgta gagcttggtg tcgcccaggt cgcggatgcg cggcgcgaag cggaagccca
     2701 agaggtgcat cagggcgaag acgtgatcgg tgaagcccgc cgtgtcggtg tagtgctcct
     2761 cgatccgcag gtcggattcg tggtacagca ggccgtcgag cacgtaggtt gagtcgcgca
     2821 ggccgacatt gaccaccttg gtgtggaatg gcgcgtattg gtcggagatg tgggtgtaga
     2881 aagtccgtcc tgggctgctg ccatattttg ggttgatgtg ccccgtgctc tttgccttgc
     2941 tagcggttcg gaaattctgt ccgtccgatg atgatgtggt gccatcgccc cagtgcccgg
     3001 caaagggatg ccgaaactga gcgttgacca gttcagccaa cgctgtcgag tacgtttcgt
     3061 cgcgggtatg ccaggcttgc agccaagcga gcttcgcgta ggtcgtgccg gggcaggact
     3121 cggccatctt ggtcaggccc aggttgatcg cgtcggccag gatcgtggtc aacaacaggt
     3181 tcttgtcctt ggccagatcg cccgatttca agtgcgtgaa gtgccgggtg aagcccgtcc
     3241 actcatcgac ttcgagcagc agttcggtga tcttgacgtg cggcaggacc atggctgtct
     3301 ggtctatcag cgcctgcgcg gtgtcgggca ccgccgcatc cagcggcgtg atcttcaagc
     3361 ccgactcggt gatgatggca tccggcaggt cgttggctgc cgccatgcgg ttgacggtgg
     3421 caagttgtgc ttccagcagc gtcagccgct catgcagata ttgttcgcag tcggtggcca
     3481 cggccagcgg caattcgctg gactgcttga ggctggtgaa cttctcgggc ggtaccaggt
     3541 agtcctcgaa gtccttgaac tggcgtgaac cctgcaccca gatgtcgccc gagcgcaggg
     3601 agttcttcaa ctcggacagc gcgcacagtt cgtagtagcg ccggtcgatg ccggcgtcgg
     3661 tcatcaccag tttctgccag cgcggcttga tgaagccggt cggtgcatcg gctggcagct
     3721 tgcgggcgtt gtcgg
//
LOCUS       DAZUFB010000234         3693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZUFB010000234 DAZUFB010000000
VERSION     DAZUFB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.238"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            270..1640
                     /gene="glmU"
                     /locus_tag="ACHY3M_005104"
     CDS             270..1640
                     /gene="glmU"
                     /locus_tag="ACHY3M_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4319814.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            1802..3631
                     /gene="glmS"
                     /locus_tag="ACHY3M_005105"
     CDS             1802..3631
                     /gene="glmS"
                     /locus_tag="ACHY3M_005105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4319815.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT          910 a          905 c         1009 g          869 t
ORIGIN      
        1 atagatcctg ctgatataaa acacccctgt tttcccgttt attcattgat cgaaataaga
       61 gcaaaaacat ccacctgacg cttaaatgaa ggtactgcct taattttctg cagacaaaag
      121 gcgtgacgat ggtcgaaaat ggcactttcg tcagcgggga taatccgtta ttgaacaatt
      181 tatcctctgt ccatttcacg atgaaaaaaa tgtagttttt tcaaggtgaa gcggtttaaa
      241 ttcgttctca aattacagtc aggacgcgta tgttgaataa tgctatgagc gtagtgatcc
      301 ttgccgcagg caaaggcacg cgcatgtatt ccgatcttcc gaaagtgctg catacccttg
      361 ccgggaaagc gatggttcag catgtcattg atgctgcgaa tgaattaggt gcagcgcacg
      421 ttcacctggt gtacggtcac ggcggcgatc tgcttaaaca ggcgctgaaa gacgacaacc
      481 ttaactgggt gcttcaggca gagcagctgg gtacgggtca tgcaatgcag caggccgcac
      541 ctttctttgc cgatgatgaa gacattttaa tgctctacgg cgacgtgccg ctgatctctg
      601 tcgaaacact ccagcgcctg cgtgatgcta aaccgcaggg tggcattggt ctgctgacgg
      661 tgaaactgga tgatccgacc ggttatggac gtatcacccg tgaaaatggc aaagttaccg
      721 gcattgttga gcacaaagat gccaccgacg agcagcgtca gattcaggag atcaacaccg
      781 gcattctgat cgctaacggc gcagatatga aacgctggct ggcgaagctg accaacaata
      841 atgctcaggg cgaatactac atcaccgaca ttattgcgct ggcgtatcag gaagggcgtg
      901 aaatcgtcgc cgttcatccg caacgtttaa gcgaagtaga aggtgtgaat aaccgcctgc
      961 aactctcccg tctggagcgc gtttaccagt ccgaacaggc tgaaaaactg ctgttagcag
     1021 gcgttatgct gcgcgatccg gcgcgttttg atttgcgcgg tacgcttact cacgggcgcg
     1081 atgttgaaat tgatactaac gttatcatcg agggcaacgt gactctcggt catcgcgtga
     1141 aaatcggcac cggttgcgtg attaaaaaca gcgtgattgg tgatgattgc gaaatcagtc
     1201 cgtataccgt tgtggaagac gcgaatctgg cagcggcctg tactattggc ccgtttgccc
     1261 gactgcgtcc tggtgctgag ttgctggaag gtgcacacgt cggtaacttt gttgagatga
     1321 aaaaagcgcg tctgggtaaa ggctcgaaag ctggtcatct gacttacctg ggcgatgcgg
     1381 aaattggcga taacgttaac atcggcgcgg gaaccattac ctgcaactac gatggtgcga
     1441 ataaatttaa gaccattatc ggcgacgatg tgtttgtcgg ttccgacact cagctggtgg
     1501 ccccggtaac agtaggcaaa ggcgcgacca ttgctgcggg tacaactgtg acgcgtaatg
     1561 tcggcgaaaa tgcattagct atcagccgtg tgccgcagac tcagaaagaa ggctggcgtc
     1621 gtccggtaaa gaaaaagtga ttctggccgg gtaatccggt cacatgggat gaggagataa
     1681 cataatctcc ctcccacaag cagtaactat aaaaataacc ccactctcta caaggctcgg
     1741 ggcgcccgaa aaaacgggca tacaggttga ccgacaacga tataaatcgg aatcaaaaac
     1801 tatgtgtgga attgttggcg cgatcgcgca acgtgatgta gcagaaatcc ttcttgaagg
     1861 tttacgtcgt ctggaatacc gcggatatga ctctgccggt ctggccgttg ttgatgcgga
     1921 aggtcatatg acccgcctgc gtcgcctcgg taaagtccag atgctggctc aggcagcgga
     1981 agaacatcct ctgcatggcg gtacgggtat tgctcatact cgctgggcga cccacggtga
     2041 accttcagaa gtgaatgcgc atccgcatgt ttctgaacac attgtggtgg tgcataacgg
     2101 catcatcgaa aaccatgaac cgctgcgcga agagttaaaa gcgcgtggct ataccttcgt
     2161 ttctgaaaca gacaccgaag taattgccca tctggtgaac tgggagctga aacagggcgg
     2221 tactctgcgt gaggctgttc tgcgtgctat cccgcaactg cgtggtgcgt acggtacagt
     2281 gatcatggac tcccgtcacc cggataccct gctggcggca cgttctggta gtccgctggt
     2341 gattggtctg ggtatgggcg aaaactttat cgcttctgac cagctggcgc tgctgccggt
     2401 gacccgtcgc tttatcttcc ttgaagaggg cgatattgcg gaaattactc gccgttcggt
     2461 aaacatcttc gataaaactg gcgcggaagt aaaacgtcag gatatcgaat ccaatctgca
     2521 atatgacgcg ggcgataaag gtatttaccg tcactacatg cagaaagaga tctacgaaca
     2581 gccgaacgcg atcaaaaaca cccttaccgg gcgcatcagc cacggtcagg ttgatttaag
     2641 cgagctggga ccgaacgccg acgaactgct gtcgaaggtt gagcatattc agatcctcgc
     2701 ctgtggtact tcttataact ccggtatggt ttcccgttac tggtttgaat cgctggcagg
     2761 tattccgtgc gacgtcgaaa tcgcctctga attccgctat cgcaaatctg ccgtgcgtcg
     2821 taacagcctg atgatcacct tgtcacagtc tggcgaaacc gcggataccc tggcgggcct
     2881 gcgtctgtcg aaagagctgg gttaccttgg ttcgctggca atctgtaacg ttccgggttc
     2941 ttctctggtg cgcgaatccg atctggcgct gatgaccaac gcgggtacgg aaatcggcgt
     3001 ggcatccact aaagcattca ccactcagtt aactgtgctg ttgatgctgg tggcgaagct
     3061 gtctcgcctg aaaggtctgg atgcctccat tgaacatgac attgtgcatg gtctgcaggc
     3121 gctgccgagc cgtattgagc agatgctgtc tcaggacaaa cgcattgaag ctctggcaga
     3181 agatttctct gacaaacatc acgcgctgtt cctgggccgt ggcgatcagt acccaatcgc
     3241 gctggaaggc gcattgaagc tgaaagagat ctcttacatt cacgctgaag cctacgctgc
     3301 aggcgagctg aaacacggcc cgctggcgct gattgacgcg gatatgccgg ttatcgtcgt
     3361 tgcaccgaac aacgaactgt tggaaaaact gaaatccaac attgaagaag ttcgcgcgcg
     3421 tggcggtcag ttgtatgtct tcgccgatca ggatgcgggt tttgtaagta gcgataacat
     3481 gcacatcatc gagatgccgc atgtggaaga ggtgattgca ccgatcttct acaccgttcc
     3541 gctgcagctg ctggcttacc atgtcgcgct gatcaaaggc accgacgttg accagccgcg
     3601 taacctggca aaatcggtta cggttgagta atcactatca tagataaaaa agggctgccg
     3661 accggtggcc cttttttata actgacatca ata
//
LOCUS       DAZUFB010000235         3588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZUFB010000235 DAZUFB010000000
VERSION     DAZUFB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.239"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..109
                     /locus_tag="ACHY3M_005106"
                     /pseudo
     CDS             1..109
                     /locus_tag="ACHY3M_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
     gene            111..404
                     /locus_tag="ACHY3M_005107"
     CDS             111..404
                     /locus_tag="ACHY3M_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFS4319816.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(465..1585)
                     /locus_tag="ACHY3M_005108"
                     /pseudo
     CDS             complement(465..1585)
                     /locus_tag="ACHY3M_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1594..2361)
                     /gene="sctT"
                     /locus_tag="ACHY3M_005109"
     CDS             complement(1594..2361)
                     /gene="sctT"
                     /locus_tag="ACHY3M_005109"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFS4319817.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2363..2623)
                     /locus_tag="ACHY3M_005110"
     CDS             complement(2363..2623)
                     /locus_tag="ACHY3M_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFS4319818.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2633..3298)
                     /locus_tag="ACHY3M_005111"
     CDS             complement(2633..3298)
                     /locus_tag="ACHY3M_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFS4319819.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3288..>3588)
                     /locus_tag="ACHY3M_005112"
     CDS             complement(3288..>3588)
                     /locus_tag="ACHY3M_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFS4319820.1"
                     /translation="DFDIKKNESKIYDENDEQMINSFEELPVKIEFVLGKKIINLYEI
                     DDLCAKRIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT         1219 a          711 c          567 g         1091 t
ORIGIN      
        1 aagagcccta aataaactta acataaaagg tactgtttag ttgtttatag cactctgtga
       61 atgggataaa cattatttta aactccaatc ttgcgtaaga gaaagttgat atgtcattag
      121 taggatataa tagtaacatc ataataacca tcgcgctgat atcgttgctc tctgcatgca
      181 ccagagaaaa ttcattaaag gagaatcaaa ataaaagaag tggcgaatcc caagcgatag
      241 tagatgcaag acagttcttt agcgaacacc cggaatatat atcatcagca gaactggagc
      301 aaaagctata tcgcgaattt gcacaggtta ctacagttcc agcttataaa gagatgagta
      361 tgtatcaatt gcttatcatt tcacaagatc gtttaactca ttaaataagg atctacacgc
      421 tttttgcgtg tagattctta taaatttctg actacgacag atgtttaatt ttttaaatta
      481 tcgtctttat tctcgatttt tgtgtcttca ccctcaatga atttatcttc tgaagagagc
      541 ggttcatctt gaacaggttg cccagcattc tccacatctt caagccacag cagaagacgt
      601 aatatttcat ctatattttc gaagctaaca taatcgtagc gacgatgggt agcataattt
      661 tttcgtgcta attttttatc tgtgataatt ggtattccga tttcctttgc atatttcctt
      721 acgcctaagg ccacctcatt agttactctt acagaaatca atggaatagg tgaaagatgt
      781 ggcttaaagt atatccctat tgcaatgtga gtagggttgg aaatcatcaa acgactatta
      841 ctgacatcag acttcaattg ctcagaaaga atttcctgat gcgctctctg cgtttagact
      901 taatttctgg atttccttct tgctccttgt attctctttt aacttcttgt ttatccattt
      961 tcatatcttt cataaataaa aaatattcag cgataaaatc aaaaattaag acaattatca
     1021 tactgccgag acaatacagt atgagaagga atagcatctc tccccaatct gataataaag
     1081 atatgatatc tccatcaaga gttttaaaaa gcagtgattt atgattactc caaaatactt
     1141 tgatcgccaa tgcaaaaaaa acaatataaa gaattgcttt aacaaattct tttacggttt
     1201 ttaagccaaa tatacgcttt aagccattta ctggattcaa tgaatcaaac ttaaatttaa
     1261 cagcttcagt tgctaattgc attttacttt gaaaccagga gacaagtatt gttgtgaaca
     1321 tgcaaaccag caaaaaagga atgttcgttt tcaaccaacc aacccccata gctttaaaat
     1381 agtcccaaat atctggttta gcgttatgat caaggatgta ttcaagaatc gacataatgt
     1441 gatgcacatc aaaaacatat ccgagatata aagtacccac aagcattatt acagagatgg
     1501 ttaagtctct actttataga aattgccctt ttttagaagc atcctggagt ttcttttgtg
     1561 ttggcttttc agttttattg gccatgaaac actctatttc tcaatgtata gttgcaattg
     1621 ttctgggaaa aaggataacg gcattactcg ttcagccagt attggggcaa aataaataat
     1681 taatattaaa aatgccagcg cacttttgac agtcagtgaa atggcaaaag catttagttg
     1741 tgaagcataa cgtgccagca atcccaggac agcctcaccg cccagcataa cggcaatcac
     1801 ggggcttgcg taaacgatag tatgagtcat tatgttatta atatatgaga ataaaggctc
     1861 taatttagga aaattaaaat tgcctgaagg ccataaatta taactttgcc aaagagtttc
     1921 cagaataaag ttcattccac ctttagtaag ataaaccgca gcagagaaaa ggttaaataa
     1981 tcttgctaat tccgaggtat cgacacctgt agccggatcc aaagtactac ttaatgttgc
     2041 accgcgttgg ttatcgataa agctacctat agcaaggaaa atccaaaacg gggatgctaa
     2101 taagcatgca ataaacagcc ctatgataat ttctctggca gcgaacaaag caatatcgat
     2161 ttcatttaag ttcaataatt catagtggta atgctgaact aatgcacatg ataccactat
     2221 tattattggg tgacgaacta ctgttggtat attcccactg gcgaaaaatg gtaacaaata
     2281 gaaagttggt gccaatcggc aaaaaccgag cgctgtagca gccagcagac tatgcaactg
     2341 atagagtatc gcctccccca tgctatcctc tcgcgaatgc taaattcagc atttcgatac
     2401 caaatgaata taatttttca ccataccacc cgctcattaa aaaaaagcaa atacttacgc
     2461 acaataattt cacgccaaat ggcaacgtct gttcttgcaa ctgcgttacc gtttgaaaaa
     2521 gccctaccaa taaaccaaca aatgtcgcaa ctgcaatagg tcctgcagac ataactaaaa
     2581 tcagatacag cgccctattc cccgcaaaaa caatatcgtc catatttttt cctcagggat
     2641 taatggatag gtcaaaatat tgtaaaatga gcccttttga taacattgtc caaccgtcca
     2701 tcgcgacaaa caaaattaat tttatcggtg tagatatggt taccggactc atcatcatca
     2761 tacctaacgt taacagaacg ctggatataa ccagatccac tacgacaaag ggtaaataga
     2821 tataaaaacc tataataaat gcacttttta tctcactcaa tgcatatgcg ggcagtaacg
     2881 agaaaattga aatattatca tcatcgatta tctcttcatt gtcttcagaa ctattcacct
     2941 tctgaatctt ttcaaaaaag ctaaccaact caggttcaga atattttatc aaatatgatt
     3001 tataaccact catgcctgtc tcaacaaaat taacaaccga tgctacatta ttgaatgaaa
     3061 gattctcatt ctgactgtta ttatatattt ccttcccaac cggcatcatt acgaacattg
     3121 acaatagcaa agcaacccca ttaagtgtca tatttgatgg cacttgctga agtcccagcg
     3181 cgtttcggac aataacaaaa acaatcgaga atttaataaa acatgtcccg gatgctatta
     3241 taaacggcaa tagtgtaaat aatgataata tcgctatcag cgaaatgcta tttgacatta
     3301 ttattccctg ataaccaaga atgaatttct acgcccaatt tatcatccac ttcaacaagt
     3361 tcgccatagc cagttagcgc accatttaca cgtatctcta tatttttctc agattcagga
     3421 agcagagata ttattctttt tgcacagaga tcgtctattt cataaagatt aattattttc
     3481 ttaccaagaa caaactcaat ttttacaggc aactcttcaa aactattaat catctgttcg
     3541 tcattttcat cataaatctt actctcgttt tttttgatat caaaatca
//
LOCUS       DAZUFB010000236         3487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZUFB010000236 DAZUFB010000000
VERSION     DAZUFB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.240"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHY3M_005113"
     CDS             <1..249
                     /locus_tag="ACHY3M_005113"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFS4319821.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGLGVRRAA"
     gene            246..428
                     /locus_tag="ACHY3M_005114"
     CDS             246..428
                     /locus_tag="ACHY3M_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS4319822.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            703..1407
                     /locus_tag="ACHY3M_005115"
     CDS             703..1407
                     /locus_tag="ACHY3M_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFS4319823.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGAEVKNIQNNYARN
                     AERFIEGKHFFKVAGDALKNLRVALNYSQNLQPSLRGLQISPKARSLILWTERGAARH
                     AKMLETDQAWEVFEKLEDCYFSQCKKNTGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WVRLKNKELPPSLHRY"
     gene            1482..2207
                     /locus_tag="ACHY3M_005116"
     CDS             1482..2207
                     /locus_tag="ACHY3M_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4319824.1"
                     /translation="MNELINSSIIKMTSIEIAELVGSRPDNVKISIERLAKSGVIQLP
                     ALQVFEKKINNLGLRRSVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGAT
                     AKIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGI
                     GPNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITG
                     RGQQWLTRKLLDNGMLKVTGEAA"
     gene            2207..2812
                     /locus_tag="ACHY3M_005117"
     CDS             2207..2812
                     /locus_tag="ACHY3M_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFS4319825.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            2809..3003
                     /locus_tag="ACHY3M_005118"
     CDS             2809..3003
                     /locus_tag="ACHY3M_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFS4319826.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            2996..3430
                     /locus_tag="ACHY3M_005119"
     CDS             2996..3430
                     /locus_tag="ACHY3M_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS4319827.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGGTFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT         1030 a          781 c          903 g          773 t
ORIGIN      
        1 atgcttgtgc cagaggatat gtctgtcgga tggttcagca aggctctgga gagtgttgac
       61 gaagttcgca tcatcactga tggacggatt aattttatcg aaccatcgac agggctggag
      121 aagaagggaa acagcaaagg ctcaatgttg ctgatttggc gaccgttcat cagtcctcga
      181 cggatgttta ctaccgtatc caaagcggca ttgatggcga tcgggctggg cgtcaggagg
      241 gctgcatgag gcgacagcga cgaagtatca ccgacatcat ctgcgaaaac tgcaaatacc
      301 ttccaacgaa acgctccaga aataaacgca agccaatccc aaaagaatct gacgtaaaaa
      361 ccttcaatta cacggctcac ctgtgggata tccggtggct aagacatcgt gcgaggaaaa
      421 caaggtgatt gacgtgatga tttattcggg gctatattcc tcacgcgcca gcaaaatctg
      481 gcgtcgggat tggcgtcctg gatagagacc gtgacagata cacgccgcga gcgtgttttt
      541 tattgtcgta tgcacgcgca catctgaatt atggtgggct gtgtgggggc ggagagatcc
      601 gcgccggtcg gtttcccggt tacgccaacc ctgcacagtt caccaccaga agattggcgt
      661 cgtcggtggt gagttattaa gaaaccactc gagggcgtca ttatgacaac tcaaatctct
      721 gttgaaactc tctccccgat cacccataac cagattcctg ttattaccac cgaacttttg
      781 gcgcagttgt acggtgctga agttaagaac attcaaaata actacgccag aaatgctgag
      841 cgctttattg aaggaaagca ttttttcaaa gtggctggcg atgccctgaa aaatttgcgg
      901 gtagctttaa actactcaca aaatttgcaa ccatctttaa gagggttaca aatttccccg
      961 aaagcccgct ccctcatcct ctggacagaa cgaggcgcag cccgtcatgc aaaaatgctc
     1021 gaaaccgatc aggcgtggga ggtgttcgaa aaactggaag actgctattt cagccagtgc
     1081 aagaaaaata ctggcaaaca agagaagagc accaacgagc tttccgcaaa agaagcaaac
     1141 agccttgtat ggttatggga ttatgctaac cgctcacagg cattattccg cgaactgtat
     1201 ccggcgctaa aacaaattca atcgaactat tccggcagat gctacgacta cggtcatgag
     1261 ttttcgtatg ttatcggaat ggcgagagat gttttaatca accacacacg agatgttgat
     1321 attaatgagc cagacggacc aacgaacctt tccgcatggg taaggcttaa gaacaaagag
     1381 ttgccgcctt cactgcatcg ctactgacaa ttgacaactt aacaaaccca gcttcggctg
     1441 ggttttttat tactgaattt tcaatgtgag aggacatgac aatgaatgag ctgataaata
     1501 gcagtatcat caaaatgaca agcattgaaa tcgctgagtt agtgggaagt cgtccagata
     1561 acgtaaaaat atcaatagag cgcctggcta aatctggagt tattcaactt cctgcattgc
     1621 aggttttcga aaaaaaaatc aataacttag ggctgcgccg tagtgtcgag gcttacgtct
     1681 tcgaaggcga acaaggtaag cgcgatagca ttattgtcgt tgcccagttg tcgccggaat
     1741 tcaccgctcg ccttgttgac cgctggcgag aactcgaagg ggcaaccgcg aaaataccac
     1801 aaaccttttc tgaggcattg cgccttgcgg ccgaccttga agaccagaag gctgaactgg
     1861 agaaacagct tgctctcgca gcacctaaag ttgagtttgc cgatcgagtt ggcgaggcca
     1921 gcggaatttt gattggaaac tttgcaaagg ttgttggtat tggtccaaac aaacggtttg
     1981 cgtggatgcg cgatcacaaa atccttattg cttcaggtgc ccggcgcaat gtgccaatgc
     2041 aggaatatat ggatcgcggc tatttcacag tgaaagaaac agcggtcaat acaaatcacg
     2101 gaatacagat atcgttcacc acaaaaatca ccgggcgtgg tcaacagtgg ctgacaagaa
     2161 agctgctaga taacggaatg cttaaagtaa caggggaggc tgcttaatgg ctaaaccagc
     2221 gcgaaggaaa tgcaaaatct gtaaggaatg gtttcacccg gcattctcaa atcagtggtg
     2281 gtgcagcccg gaacacggaa ctaaattagc actcgaacga cgaaataaag aacgcgaaaa
     2341 ggcggaaaaa acagcagaga agaaacgacg acgagaggag cagaaacaga aagataaaat
     2401 taagattcga aaactcgcct taaagccccg cagttactgg attaaacaag cccaacaagc
     2461 cgtaaacgcc ttcatcagag aaagagaccg cgacttacca tgtatctcgt gcggaacgct
     2521 cacgtctgct cagtgggatg ccggacatta ccggacaact gctgcggcac ctcaactccg
     2581 atttgatgaa cgcaatattc acaagcaatg cgtggtgtgc aaccagcaca aaagcggaaa
     2641 tctcgttccg tatcgcgtcg aactgattaa tcgcatcggg caggaagcag tagacgaaat
     2701 cgaatcaaac cataaccgcc atcgctggac tgtcgaagag tgcagggcca tcaaggcgaa
     2761 gtatcagcag aaacttaaag acctgcgaaa cagcagaagt gaggccgcat gacgttcaca
     2821 gtaaaaacca ttcctgacat gctcgttgag gcatatgaaa atcagaccga ggtagccaga
     2881 atactgaact gtagtcgcaa cacggtcaga aaatacactg gcgataaaga aggaaaaaga
     2941 cacgctattg tcaacggtgt tcttatggtt catcgcggat ggggtaaaga tactgatgcg
     3001 tgatatccgg caggttcttg agtgctgggg ggcatgggcg gcaaataacc atgaggatgt
     3061 gacctggtca cccattgccg ccggatttaa gggactgatc cccgaaaaag taaaatcacg
     3121 cccgcagtgc tgtgacgatg atgcgatggt gatatgcggg tgtatagccc gcctttaccg
     3181 gaacaatcgc gatctgcatg acttgctggt tgattattac gtgctggggg ggacgttcat
     3241 ggcgctggca cggaaacatg ggtgctctga cacctgtata ggtaaacgcc ttcacaaagc
     3301 ggaggggatt gttgaaggca tgctgatgat gctgggagtg aggcttgaga tggatcggta
     3361 tgttgagcgt gaattgccgg gagggagaac ctctgtattt tatcagcgaa aaaatagttt
     3421 acgatcgtaa aaatctgcat atcatgataa gagtggttac attgccacgc tgcttaaccc
     3481 gccgatg
//
LOCUS       DAZUFB010000237         3373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZUFB010000237 DAZUFB010000000
VERSION     DAZUFB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.241"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..500)
                     /locus_tag="ACHY3M_005120"
     CDS             complement(<1..500)
                     /locus_tag="ACHY3M_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4319828.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFGAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSG"
     gene            complement(650..1087)
                     /locus_tag="ACHY3M_005121"
     CDS             complement(650..1087)
                     /locus_tag="ACHY3M_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4319829.1"
                     /translation="MSRVTAIISALVICIILCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGFTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1084..1581)
                     /gene="rrrD"
                     /locus_tag="ACHY3M_005122"
     CDS             complement(1084..1581)
                     /gene="rrrD"
                     /locus_tag="ACHY3M_005122"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFS4319830.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1581..1796)
                     /locus_tag="ACHY3M_005123"
     CDS             complement(1581..1796)
                     /locus_tag="ACHY3M_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4319831.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1939..2337
                     /locus_tag="ACHY3M_005124"
     CDS             1939..2337
                     /locus_tag="ACHY3M_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319832.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(2418..2576)
                     /locus_tag="ACHY3M_005125"
     CDS             complement(2418..2576)
                     /locus_tag="ACHY3M_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFS4319833.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(2662..3373)
                     /locus_tag="ACHY3M_005126"
                     /pseudo
     CDS             complement(2662..3373)
                     /locus_tag="ACHY3M_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          985 a          841 c          656 g          891 t
ORIGIN      
        1 cctgacatgc tggcgacatc cttctctttc atgtactcca gcattacggc gttacgttct
       61 gcttcataac tttcgctgta cttacggagt tctgctgaca tccagttaaa cgcattgata
      121 taggcttctt taacagcatc ggcttttgcc ccgttaaatc ccataaccag cataacgaag
      181 ccactaaagt ccatgcggta gtaaatctgt ttcttgtcaa aaatccctaa gtcattgatt
      241 ttctcgacgg ccccaaaatg ggttgtcgag aattcctcag aacatctcaa acatttaata
      301 gccctaatca catgctgatg gcgtttacca aatgccctgg caatctggaa ggtgtcagtt
      361 accggttgac cttctgctgc ggtaactaac tggcgaaagt cgaagtcatg attcgcaatt
      421 aattcattca tggcgttgcc tgcttctttg aaatgaacct ttgccgcaca ggaaaccagc
      481 ccaccgaggc tcgccagcac taactggtat cctcaaaggc ccattccaaa gggtcaggtt
      541 cggtgtttat tgtgcgctgc ggtgcgcggt gaaataccgg tacaaaaatg ccccgcatct
      601 gcgaggcatt ttcctgaaag tcacttgtta aatttcagtg aaattaaaat tattttaagc
      661 actgcgtcct gatgtattcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac
      721 ctcggagacg gtaataattg agttcagcat ctgctgtaaa tcctgggctt tctccatcgc
      781 ccatgctgct ggctccggtc gttgactttg cacaggtggc ggagacttgc aggcgcttac
      841 gaccagcaga aacatcggca cggagacttt cgatagtcgc gttagcatca gcaagttcct
      901 ttgtatatct tgcgtcgagt tctgctacat cacgttgacg cttctgcata tcagcgatga
      961 tggatgtggc tttatcgcgc tgctctttgt aggcgatggc gttatcacgg taatgattaa
     1021 cagcccatga caggcagagg atgatgcaaa taaccagagc ggagataatc gcggttaccc
     1081 tgctcattgc tgcccccaca aacagacttc acgctcaatc tcacgacgag tcatcaggcc
     1141 tttccattgc ttaccgccag cgtatgtcca gcgacgtagc tggtcacatg cgcctttgat
     1201 atcgccctgg tttattttgc gaagaagcgt cgatgttctg aaattaccag cgcccacgtt
     1261 gtaaacgaac gagtaaagag cgccgcgcgt tgtttccggt atatcgactt tgatgtacgg
     1321 gttaatttgt ctggcgaccg tggcaaggtc tttattcagg agggctttgc attctgcttc
     1381 ggtatacgtt ttaccgggaa tgatgtcttt tccggtgtgt ccgtgacata cagtccatac
     1441 gccaacgata tcttcgtatg gtatgtagct gacaccttcc aggccatcgt caccactcgg
     1501 accagtgatg agcacagacg ctatggcaac agccccacca ccaatagcag cagcaacagc
     1561 cttgcgtaat gatggcgaca ttattcacct ctcgcagcct tacgcttatc ttctttaatc
     1621 ttgaaataaa ggtttgtcag atacgtcagc aagccaaaca ccaggctacc caatacgcct
     1681 attgccaccc actgggatgg ggagactttg tccagcaact gcagtagcca gtatcccgtc
     1741 cccaccgctg acgtggtgta tgacacacct gttgttattt tttccatctg gtacataccc
     1801 cgtctcccgt tatccggaag ctgacaacaa taaaaagcca ccagttaatt cctgatggcc
     1861 ctgatgcata aacgtcataa tacctgactg ttatgattga caataatgat aatgtttata
     1921 tagaaaggtt cccgatgtgt gttacatatc atttctccac ggggaatatc cccacgccag
     1981 cgcagactct tttacccgtt ctcttctgcg ctggctcttt tttattatgc tgctgcattt
     2041 acctctggca ccatgctttc tatctcaaca caatacgtgg tacttcttgt aaccaatatc
     2101 ataacgatta atcgacatag aatttctccc gtgtacagga acagagttaa aaagccggaa
     2161 ccggaatcaa atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggccgcgc
     2221 catgcgcagc cagaactcac aaggaaaatg atagaaggaa ataacattag tgatgtacgc
     2281 atggcgcctc ccgctaagtt ctgcaatgat caaacagaac tcgctacgtg cccttaaaac
     2341 tcgatcattt agcccctcca aggaggattc accatgcggt tgatttttta ataaacagta
     2401 aacaaaaaag tcaagaatta ttcattctgt tctttcatca tcggccacag caataccaca
     2461 atgccgcaga ccagagcgcc atcagtcagt accaacatta tcctgctggt gaaatccatc
     2521 atcaccatca ctaaaagcag gatcacaaca gcaagcagac acagtttata aaacaatgtt
     2581 cagaaaacgc attcagcatg cctaaggttc tattcctacg aatagccaac ttgcaactta
     2641 aaatattatt tatgcagcca attaaattct ggtccttaca atatcaacct gaagattctt
     2701 atcttgtgct gattgataaa tgacaaacct tttactacct gcattgaaag aagtagacaa
     2761 aaccagacaa ttatcataac gagcaagaac ataataccaa ccatcattat aattaatcat
     2821 ttcatattct ttcttaaact gtggtttgta atatcctgtc agaaatgaaa aaagccagaa
     2881 atatgccaga aatatgccac aaaagcaatc atcacaatct caaaaaaatg tttttttata
     2941 aatggcttat catagaagca tgataccgat aaaaatcgcc cataagatct tatcgaaatt
     3001 gtaaccgcca gcgcaatcgc tgctgacagt agcaaaagag gtacctgaat cttctgtctc
     3061 aatatagaaa actcaataat tgccggcaca aacaataatt ccacagcaaa ataaaggcga
     3121 aatacattta gctcttgcat agaatgtttt cttttcactg cgaaaaagaa tacaacacca
     3181 ataccccaac cgataagaaa tatagcaatg acgataactg caaaaaataa acttctggca
     3241 acatcatcaa cacctgcacc tacaatccac catgggaagc cgtagtaaaa agaagtaccc
     3301 catccataga aataagcact cccccatcca aggcatccca tgtaggcaat aaaaagtgaa
     3361 gaactcctga gca
//
LOCUS       DAZUFB010000238         3367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZUFB010000238 DAZUFB010000000
VERSION     DAZUFB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.242"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1058)
                     /gene="escV"
                     /locus_tag="ACHY3M_005127"
     CDS             complement(<1..1058)
                     /gene="escV"
                     /locus_tag="ACHY3M_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFS4319834.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEP"
     gene            complement(1055..1408)
                     /gene="cesL"
                     /locus_tag="ACHY3M_005128"
     CDS             complement(1055..1408)
                     /gene="cesL"
                     /locus_tag="ACHY3M_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFS4319835.1"
                     /translation="MNLLVKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDNN
                     VFVTFALEYNENAFFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTF
                     QQHVQIIERVRRMTS"
     gene            1593..1889
                     /locus_tag="ACHY3M_005129"
     CDS             1593..1889
                     /locus_tag="ACHY3M_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFS4319836.1"
                     /translation="MEAANLSPSGTVLPLAATINGNNLVDEKTGVMQPEGETSRNVRI
                     LAGVLISVGTLAAIGTGIASLCVEDSSQRLGLGIAAGVLGGVTTVAGGLAMRYA"
     gene            1972..2349
                     /gene="sctI"
                     /locus_tag="ACHY3M_005130"
     CDS             1972..2349
                     /gene="sctI"
                     /locus_tag="ACHY3M_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFS4319837.1"
                     /translation="MNNINQSKNINIQLNKAFQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            2352..2924
                     /gene="escJ"
                     /locus_tag="ACHY3M_005131"
     CDS             2352..2924
                     /gene="escJ"
                     /locus_tag="ACHY3M_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFS4319838.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            2930..>3367
                     /gene="sepD"
                     /locus_tag="ACHY3M_005132"
     CDS             2930..>3367
                     /gene="sepD"
                     /locus_tag="ACHY3M_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFS4319839.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSKEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQSSSSDTMMPPTHLFSD"
BASE COUNT         1176 a          670 c          621 g          900 t
ORIGIN      
        1 aacggctcgg cgcctggtga gatgttttgg ctattagaca aatcagctcc catagcatcc
       61 ttattattgc cagttccaaa tgttcttttc ttctgtaatt tccaagctat ccccaacagg
      121 caaaccgcca gaaataagaa gaccaatgta ggaaagcccg gaataatcgc cattacgagc
      181 attagtgcgg cactgattaa aaaagggcga ctattaacag aaacctgttg aacaatatca
      241 cctgcaaggt tttcttcttt ttcgctttca ccgggcacac gagtaacaac cacgcccgcg
      301 gtgacagaga taataagtgc agggatctgg gcaaccaaag catcaccgac agacaataca
      361 gaaaacaggc taagtgcctc actaaatggc atatcaaatt gccacatacc aatgagcacg
      421 ccaccaaaca ggttaactaa aacaataatg ataccggcaa tagcgtcccc ttttacaaac
      481 ttcatcgccc cgtccattga accataaaac tggctttcct tttgcactcg ggaacgaaga
      541 acttttgcct caagcgggtc aataacgcct gcgcgcatat caccatcaat actcatttgt
      601 tttcctggca taccatcaag ggaaaaacga gcacttactt cagcaacacg ctcggcccct
      661 tttgtaataa ccataaattg aacaatggtg ataatagtaa aaataaccag gccaacaaca
      721 atattaccgc caacaacaaa gttaccgaag gagtaaataa tgtcacccgc atcgtgatgc
      781 agcagaataa gtctggttgt actaacactg agcgacaggc gcatcaacgt cgtaatcaac
      841 aggattgtgg ggaaagaagt tagttcaaga ggatttttta tatagattga gagcattaat
      901 aaaagtaaag ctgtcgaaat attaatcgcg ataatgatat ccaccacaac tgttggtagt
      961 ggaataatca tcatcattac agccataaag aagaagagag ccagaataag atcgtgatat
     1021 gactctgctt ttttaaatat atttaagagt ttattcatga tgtcatcctg cgaacgcgct
     1081 caataatctg cacatgttgt tgaaaagtat tcattagaaa aaaaacatcg atattggtta
     1141 acaaacaact taagcacaat ttattatcgt agacaaaaat atgttcaatc actccctgcg
     1201 tacgctcagg agcaagggca ctaaagaaag agaaaaatgc gttttcgtta tactctaaag
     1261 caaacgtaac aaaaacatta ttatcaatta tttcaataaa aaaagaaaac ccattaaaat
     1321 caatttcaac acggttatca aataaataaa aatcatttcc caacaatctt aaaaactctt
     1381 caacgtttct tttaactaaa agattcatct gcaggctctg aagtaagtat acgtgtgaat
     1441 attattgctt gaaaaagcgt attggataat atatacagta tatgtacttt atgtacattg
     1501 caactattta aataatataa tagttgccta tgggataatt tggttattta taaccaagta
     1561 aatgtctaaa ttagacaaaa ggagaaagat caatggaagc agcaaattta agtccttctg
     1621 gtacagtatt accactggca gccacaatca atggaaataa tctagtggat gagaaaacag
     1681 gagtgatgca accagaaggt gaaacaagtc gtaacgttag aatcctagct ggagttttga
     1741 taagtgtagg tactctagcc gctatcggta ctggtatcgc atcactgtgt gttgaagact
     1801 cttcacaaag attaggctta ggaatcgcag ccggtgtatt aggtggcgta actactgttg
     1861 ccggcggatt agcgatgaga tatgcctaat catcgcatcg cataaaaaaa tagagaggta
     1921 atggatgcat tatgccattg cctctctcat gaaaagagac taacagtaaa tatgaataat
     1981 attaatcaat caaaaaacat taatattcag ttaaataaag cattccaaac aaatgtcgtt
     2041 gatgagcata tcccgttagc atcaaccccc tctgcagccg gagcggctca gtttcttgat
     2101 caattactcc ctaaaacggc gggagtgtct tccccagaac aagtgttgat tgaagaaatt
     2161 aaaaagcgac atcttgcaac aatgaacacc gatctcagtt tcgatgcact atctgcaggt
     2221 gggctctcgc ccgaagatgt actcacttta caaaagaatg tgctcaacgc aaacgtcaat
     2281 gttgatgtcg tatctaagtt agcaagcctc ctttcaacat cggttacaaa attagtttcg
     2341 atgcaataaa aatgaaaaaa cacattaaaa accttttttt attggctgct atttgtttga
     2401 cagtggcatg taaagagcaa ttgtatacag gattaacaga aaaagaagct aatcagatgc
     2461 aagcactgtt gctatccaat gatgtcaatg tttccaaaga aatggacaaa agtggaaata
     2521 tgacactcag tgtggaaaaa gaggattttg ttcgcgcaat aacgatttta aataataacg
     2581 gcttcccgaa aaagaaattt gcagatatcg aggttatttt cccacccagc cagttagtgg
     2641 catcgccatc acaagaaaat gcaaaaatca actatctcaa agaacaagac atcgaaaggt
     2701 tgctaagtaa aatacccggt gttatcgatt gcagcgtttc attaaatgtt aacaataacg
     2761 aaagtcaacc atcctcagca gccgttttag tcatcagctc gccagaggtt aacttagcac
     2821 catcggtcat tcagataaaa aacctggtta agaatagtgt cgacgatctc aaattagaaa
     2881 atattagtgt tgttatcaag tcatcctcag ggcaggacgg gtaatatgta tgaacaataa
     2941 taatggcata gcaaagaatg attgtgactg gctaacggcc cttgattttg taaaggatgt
     3001 caacggttca ccaacgcatc tcacttttta tatctaccaa aaaaatgctt tcttgcacga
     3061 ttttggaaac tactgggttc tatatatcga gcttagtggt gattttcgtc aggttccaac
     3121 agatacattt atcagactct gcaatatcct ggccgttagt aaagaatata aacaaatggg
     3181 tattttcctg tctaataaga agtggtatct ttgccaaatt tttcataaag ataataatca
     3241 tcgcgcaaac atgtcaaaag caataatgca acatactctc gcctcgttgc tcgacaagca
     3301 atttgataaa ctagagcaat catcttcatc agatacaatg atgccgccaa cacacttgtt
     3361 ttctgat
//
LOCUS       DAZUFB010000239         3254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZUFB010000239 DAZUFB010000000
VERSION     DAZUFB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.243"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(112..639)
                     /gene="mobB"
                     /locus_tag="ACHY3M_005133"
     CDS             complement(112..639)
                     /gene="mobB"
                     /locus_tag="ACHY3M_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFS4319840.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(621..1205)
                     /gene="mobA"
                     /locus_tag="ACHY3M_005134"
     CDS             complement(621..1205)
                     /gene="mobA"
                     /locus_tag="ACHY3M_005134"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFS4319841.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1275..1544
                     /locus_tag="ACHY3M_005135"
     CDS             1275..1544
                     /locus_tag="ACHY3M_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFS4319842.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1621..2607
                     /gene="srkA"
                     /locus_tag="ACHY3M_005136"
     CDS             1621..2607
                     /gene="srkA"
                     /locus_tag="ACHY3M_005136"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFS4319843.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2624..3250
                     /gene="dsbA"
                     /locus_tag="ACHY3M_005137"
     CDS             2624..3250
                     /gene="dsbA"
                     /locus_tag="ACHY3M_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFS4319844.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          825 a          783 c          792 g          854 t
ORIGIN      
        1 tgccaggcat caaattaagc agaaggccat ccgtcaggat ggcctttttg cattggcgca
       61 gaaaaaaatg ccggatgcga cgctggcgcg tcttatccag cctactcttg cttatccgtt
      121 ctgcttttgc atccactcca ccacaaaatc agccagcccc tcaacatcat taatatccag
      181 taatgcgaca tcaagattaa gcggcacatc actggctaca gcaataacat gcctgtctat
      241 cactaattct tcaggtcgat gtccggctcc atcgcgaaac agcacaatct ttgcgatctc
      301 ttcatgctta aacccttcga ccagaatcaa atccagcttt gaggtatcca ttcgacttgc
      361 gagaaactgt agatccagct cttcttcgtc tggtgtttcc gtcatcaagg cccatcgttg
      421 ctggctggca acgatggttt gtgccgcgcc agccttgcgc agctcatagc tatctttgcc
      481 tggcttatca acatccatat catggtgcgt atgcttaatc agccctggac ggatccctct
      541 ggcgcataat gccgggatca gttgtttcaa aagcgtagtt tttccggtac cactccacgc
      601 ggcaaaggcg agtaacggta tcatcgtttt tcctgccatc gggcaagctc ctctggcgta
      661 ttcacgttaa taaatgcatc tttattatcg ctgaaatcaa ccgcatgacc gccagccaga
      721 cgcataaatg ccattacccg gcgttctcct gcttgcagat attccagtaa taaaggctca
      781 attgcgcggt ttaccagagc aatagtcggg tgatcgcgtt caccgtcgtg gacccacacg
      841 acaggcgcat ctttgcgctg atgattaagc cgggctgcta aatcctcggg aatataagga
      901 gtatcgcacg gacaaaacaa aaaccactcg cctgcttcct gctgcattac tgaaagcatt
      961 cctgccagag ggcctgggta atccgccagt gaatcttcaa tcactttcag accgcttgcc
     1021 tgatagattt cctgatggcg attggcatta accacgacgt gagagagctg cgtcataagc
     1081 gcgtcagcga catgttgcca taatggtttg ccgtttaatt caagcaatcc tttatctacg
     1141 ccgcccattc gtctggcttt accgcctgcc agcacaacgc ctgttatcgt cgtcatcaga
     1201 ttcaccgata tcgcctcttt tattgtggga ttgaccctgc taacgtgtct gtctcaagag
     1261 taaggagcat tactatgaaa tgtaaacgtc tgaatgaagt tattgaactc ctccagccag
     1321 cctggcaaaa agagccagac cttaacctgt tgcaattttt gcagaaactg gcgaaagagt
     1381 caggttttga cggcgaactg gcggatttga cggatgacat tctgatctat cacctgaaaa
     1441 tgcgtgattc cgcgaaagat gcggtgatcc cgggtttgca gaaagattat gaagaagact
     1501 tcaaaacggc gctgttacgc gctcgcggcg taattaaaga gtaaaagctt gtaagcggtg
     1561 ccaccaaaat catcgtgaaa tgatatcctt catcattcgt aatgttttcc ggatgatggg
     1621 atgaacaaca gcgcttttac tttccagaca ctacacccgg ataccatcat ggatgctcta
     1681 tttgagcaag ggatccgggt ggattccggt cttaccccgc ttaacagcta tgaaaaccgt
     1741 gtctatcaat ttcaggacga agatcgtcga cgttttgtcg tcaaatttta tcgccctgaa
     1801 cgttggacag ccgatcaaat cctcgaagaa catcaatttg cgttgcagtt ggtaaatgat
     1861 gaagttccgg tcgcagcacc tgtggccttt aacggtcaga ctttattgaa tcatcaggga
     1921 ttttatttcg ctgtttttcc aagcgtcggt ggtcgccagt tcgaagctga taatatcgat
     1981 cagatggaag cggttgggcg ttatttaggg cgtatgcacc agacggggcg caaacagctt
     2041 tttatccatc gcccgaccat cggtctgaat gaatatctca ttgagccacg caagctgttt
     2101 gaggacgcta cactgatacc ttccgggttg aaagcggcat tcctgaaagc gacagatgag
     2161 ctgatcgccg ccgttacagc gcactggcgg gaagatttca ccgttctgcg gctacatgga
     2221 gactgccacg ccgggaatat tctctggcgc gatggtccaa tgtttgttga tctggatgat
     2281 gcacgtaatg gtccagctat tcaggatttg tggatgttgc tcaatggcga taaagccgag
     2341 cagcggatgc aactggaaac tattattgaa gcttatgaag aatttagcga gttcgacacc
     2401 gctgaaatcg gactgattga acctttacgc gccatgcgtt tggtttatta tcttgcctgg
     2461 ctaatgcggc gttgggctga tcccgcgttc ccgaaaaatt tcccgtggtt aaccggggaa
     2521 gattactggc tgcgacaaac ggcgactttt atagaacagg caaaagttct acaagaaccc
     2581 cctttacaat taacacctat gtattaatcg gagagagtag atcatgaaaa agatttggct
     2641 ggcgctggct ggtttagttt tagcgtttag cgcatcggcg gcgcagtatg aagatggtaa
     2701 acagtacact accctggaaa aaccggtagc tggcgcgccg caagtgctgg agtttttctc
     2761 tttcttctgt ccgcactgct atcagtttga agaagttctg catatttctg ataatgtgaa
     2821 gaaaaaactg ccggaaggcg tgaagatgac taaataccac gtcaacttca tgggtggtga
     2881 cctgggcaaa gatctgactc aggcatgggc tgtggcgatg gcgctgggcg tggaagacaa
     2941 agtcactgtt ccgctgtttg aaggcgtaca gaaaacccag accattcgtt cagcatctga
     3001 tatccgcgat gtatttatca acgcaggtat taaaggtgaa gagtacgacg cggcgtggaa
     3061 cagcttcgtg gtcaaatctc tggtcgctca gcaggaaaaa gctgcagctg acgtgcaatt
     3121 gcgtggcgtt ccggcgatgt ttgttaacgg taaatatcag ctgaatccgc agggtatgga
     3181 taccagcaat atggatgttt ttgttcagca gtatgctgat acagtgaaat atctgtccga
     3241 gaaaaaataa ttca
//
LOCUS       DAZUFB010000240         3204 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZUFB010000240 DAZUFB010000000
VERSION     DAZUFB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.244"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..401)
                     /locus_tag="ACHY3M_005138"
     CDS             complement(36..401)
                     /locus_tag="ACHY3M_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319845.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPGVLAGEMCTEISSELLPVIREAICRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
     gene            complement(413..565)
                     /locus_tag="ACHY3M_005139"
     CDS             complement(413..565)
                     /locus_tag="ACHY3M_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS4319846.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            complement(758..1165)
                     /locus_tag="ACHY3M_005140"
     CDS             complement(758..1165)
                     /locus_tag="ACHY3M_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319847.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            1243..1470
                     /locus_tag="ACHY3M_005141"
     CDS             1243..1470
                     /locus_tag="ACHY3M_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4319848.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            1454..1975
                     /locus_tag="ACHY3M_005142"
     CDS             1454..1975
                     /locus_tag="ACHY3M_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4319849.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITESFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            1956..2921
                     /locus_tag="ACHY3M_005143"
     CDS             1956..2921
                     /locus_tag="ACHY3M_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319850.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GNELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            2962..3204
                     /locus_tag="ACHY3M_005144"
                     /pseudo
     CDS             2962..3204
                     /locus_tag="ACHY3M_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
BASE COUNT          878 a          676 c          828 g          822 t
ORIGIN      
        1 ggaccgctat atcaaaaggt tagaggacgc agggatcaaa tccccagtga agtcaccatt
       61 gtcctgagaa cttgcaggac gtcatgatcg taacttccat ccaaaccgcg acggcaaatt
      121 gcttctcgta ttaccggaag cagttcgctg gaaatctcgg tgcacatttc acctgctaat
      181 acgcctggct caagtgagaa tattggtgaa cttacggtct tggtctcgac agtttcagag
      241 tcagtgccaa cattataaag ctcaacgaaa gcggtcttga ttttccgggc cagatctttt
      301 gctggctcgc ttgcaatatc tttcccgatt tctcgcagca cagaatgcaa tgtatgagct
      361 gctgttttct gtacatcaga cggtaaatct ttaaattcca tcgtcagcct catcagtcag
      421 tgtttctggc taaccagcaa cgcgcgccag attcggtttt aaacgttttg cttttggtat
      481 atgtcatcgc ggtgaacgta ccgtcctggt tggggaacac gccacatacc agagattcgc
      541 tgttgccaag atcgatagta tccatgctga cctcatttcc ccttaacgcc ggggtagcgg
      601 aactgtttgc tgagaacacc gtgcggtgtg ttgatggagg taatttagtt ttctcattat
      661 ttttcgtcaa gattttttga tgagaaaact caagtattgg cgcaagataa agccaataca
      721 ttgaaatgta aggctttaaa attttgtgaa gggatgatta ttgatgcttg ttgcgtttgc
      781 gagcctctaa tagctcggtg aacaggcgat taaaattctc aacgcgggcg cggagttcgc
      841 tgagttgtgc ttgttgctct gatttaggaa gtgcgcgata caaacgcagc atctctaact
      901 catcttccga taagtctaag gcactatcga gtgaaacagg gggagctggt gttttgtcct
      961 cgtcgccaaa cagtatccag gttggtgaac attgcaatac ctcggcgagg cgatgcaaat
     1021 tttgcccacg cggggctgta tggtcacttt cccataatga aattgatgag ccagatacgc
     1081 cagcagcttt gcttaaaccg ttttgactta agcctacctg cttacgtctt tctctaatgc
     1141 gttgacctaa agttttctcg ttcatattta gatatcttaa taacccttga cttgagattc
     1201 cttgagtgat tagctttgag aaaactcaat attggaggtg cgatgtttaa atcagacgta
     1261 attaattttt acggtacgaa agccaaagta gcgaaagctg ctggcgttga cccatctgct
     1321 gtttctcaat ggcaagagct ggttcctgaa ggtcgcgcga tgcgtctaca ggaggcatct
     1381 ggcggcgagc ttctgtatga tcccaaggtt tatgacgaat atcgtaagac gaagcgggcg
     1441 gggcggttga acaatgaaaa tcactcctga acaggctcgt gaggctctgg atgcctggat
     1501 atgtcgacca ggaatgacac aggagcaggc gacgatatta atcactgaat cattctgggc
     1561 tttgaaagag cgcccgaaca tcgatgttca gcgtgtcaca gatgaaggtg gcgcggttga
     1621 tcagcgagcg cttggcgtta atcgagtgaa gatattcgaa cgctggaagg ctatcgacac
     1681 tagggataag cgtgaaaagt tcacggcgct agtgcctgca attatggagg ctatccggat
     1741 taatgatttc aggttgtatc gtgaaattag tgacggaaaa agcatcacgt acatgatcgc
     1801 cgggttaaac aaagaatatg gcgatgtggt ggagtccgga ctgctttttg ctgatcctgc
     1861 cgtagtggat cgtgaaactg acgaacttat agaaaaagca attgctttca aacttgcgta
     1921 tcgacagcaa taccaacaaa aagctggatg gaattatgag ccttcttttt gctgaacgcc
     1981 cactggttat aaacacacag cttgcgatga agattggctt aaatgaagcc attgtgttgc
     2041 agcagttgca ttactggttg agagatacca attccggcat ggaatgtgat ggtgttcgct
     2101 ggatttacaa cacaacggaa caatggctgg aacagttccc attctggtca gagtcaacgt
     2161 taaagcgcgc gtttgcaagt ctgaaaacgc tggggctttt gcgttgtgaa aagctcaata
     2221 aatcaaagcg cgatatgacc aatttctaca cgatcaacta tgggaacgag cttttagatg
     2281 gtggcaaatt gaacgaatcc atcggttcaa aatgcgccgc tccatcaggt caaaatgaca
     2341 cgatggaaga ggtcaaaatg aaacgctcca ttggttcaaa acgacccaat gtcatcgggt
     2401 caaaatggcc tgatgatctt acagagaata caacagagat tactacagag aataaaaaca
     2461 cttttcgtcc ggaagcttcg caaccggacc cgcagaccgc tgaacaggat tttttaatcc
     2521 ggaaccccga cgcggttgtg tttagtgcga aaaaacgcca gtggggtagc agggaggatt
     2581 tggcgtgtgc gcagtggatt tgggggcgga tcgtgaacct ttacgaacag gctgccagcg
     2641 acgatggaga gatcatgcga ccaaaagagc ctaactggac agcctgggcc aatgacgtgc
     2701 gcacaatgcg gatgctggat ggcagaagcc acagacaaat ttgcgaaatg tttggtcggg
     2761 tacagcgaga tccattctgg gtaaaaaaca tcatgagccc gtcaaaactc cgcgaaaaat
     2821 gggacgaact ggtcatccgc ttgggacgtt cacctgtaca gcgttgtgtg aatcatattt
     2881 ctgaaccgga tacagaaatt ccgcctggtt tcaggggata agtgttgatt tcaggtcatg
     2941 aggtaatttt aaggaggact tgtggcaaaa gtttttacac aggaagagcg ggaaaaaaat
     3001 taaagagcag gtggtcgaac ttgtgcgcca gagcggtcgc gagacattac gacagttgga
     3061 agctaaaaca ggtgcgacaa gatatctgat gagcattctt gccagagagc tggttgccag
     3121 tggttatgta tacaactccg gctacgggtt attcccgtct gaacaggcac gaaaggactg
     3181 gcaaaatgcc cgcaaaaaac tctc
//
LOCUS       DAZUFB010000241         3169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZUFB010000241 DAZUFB010000000
VERSION     DAZUFB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.245"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            169..501
                     /gene="hdeA"
                     /locus_tag="ACHY3M_005145"
     CDS             169..501
                     /gene="hdeA"
                     /locus_tag="ACHY3M_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFS4319851.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            617..943
                     /gene="hdeB"
                     /locus_tag="ACHY3M_005146"
     CDS             617..943
                     /gene="hdeB"
                     /locus_tag="ACHY3M_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFS4319852.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1007..1654
                     /locus_tag="ACHY3M_005147"
     CDS             1007..1654
                     /locus_tag="ACHY3M_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4319853.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1696..2226)
                     /locus_tag="ACHY3M_005148"
     CDS             complement(1696..2226)
                     /locus_tag="ACHY3M_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS4319854.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2382..2948)
                     /gene="slp"
                     /locus_tag="ACHY3M_005149"
     CDS             complement(2382..2948)
                     /gene="slp"
                     /locus_tag="ACHY3M_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFS4319855.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          927 a          622 c          682 g          938 t
ORIGIN      
        1 aatttcctaa ttgcagtatc tgatgcatct gtaactcatt gtattgaaat aaaaatatct
       61 gattttgata ttttccatca acatgacata tacagaaaac caggttataa cctcagtgtc
      121 gaaattgatt cgtgacggct ctttcacttt atagttgagg atattacgat gaaaaaagta
      181 ttaggcgtta ttcttggtgg tctgcttctt ctgccagttg tgagcaatgc agcggatgcg
      241 caaaaagcag ctgataacaa aaaaccggtc aactcctgga cctgtgaaga tttcctggct
      301 gtggacgaat ccttccagcc aactgcagtt ggttttgctg aagcgctgaa caacaaagat
      361 aaaccagaag atgcggtttt agatgttcag ggtattgcaa ccgtaacccc agctatcgtt
      421 caggcttgta ctcaggataa acaagccaac tttaaagata aagttaaagg cgaatgggac
      481 aaaattaaga aagatatgta attccgggaa tgcgttacat cgtacttcct tgcatattga
      541 acaggccgga atatcttctt taaaagcagc tattcctcct gttcatatat aatctctata
      601 ttgaatgggt tacaaaatga atatttcatc tctccgtaaa gcgtttattt ttatgggcgc
      661 tgtagcggct ttgtcactgg tgaacgcaca atctgcgctg gcagccaatg aatccgctaa
      721 agatatgacc tgccaggaat ttattgatct gaatccaaaa gcaatgaccc cggttgcatg
      781 gtggatgctg catgaagaaa cagtatataa aggtggcgat accgttactt taaatgaaac
      841 cgatctcact caaattccta aagtgatcga atactgtaag aaaaacccgc agaaaaattt
      901 gtataccttc aaaaatcaag catctaatga cttgccgaat taatgaggta caagtaaaaa
      961 ggagtagcaa gttgagccat cttgctgctc ctttttgcat ttttatatga cagcagaatt
     1021 tattatacgt cttatactcg cggcaattgc ctgtggcgct attggcatgg aaaggcaaat
     1081 gcgcggcaaa ggagcagggt tacgcacaca tgtattaatt ggcatgggaa gcgccctgtt
     1141 tatgattgtt tcgaaatatg gttttgctga cgtgctgtct ttagatcacg tcggactcga
     1201 ccccagccgt attgctgctc aggtggtgac gggcgtcggg tttatcggtg caggtaacat
     1261 tctcgttcgt aaccaaaata ttgtaggtct gacgacggca gcggatatct gggtgaccgc
     1321 cgccataggt atggttattg gcagcggtat gtacgaactg ggtatttatg gttcagtgat
     1381 gaccttgttg gtgctggaag tcttccatca attaaccttc cgcctgatga acaaaaatta
     1441 ccatttacag ctgacattag tgaacggtaa tacggtatca atgctcgact ggtttaagca
     1501 gcaaaaaata aaaacggatt tagtttcgtt gcaggaaaat gaagatcatg aagtggtcgc
     1561 cattgatatc cagcttcatg ccacaacgtc gatagaagac ctgttgcgat tattaaaagg
     1621 tatggcaggg gtgaaaggcg tttcaattag ctaatctgtg tggcagcgta gccagactca
     1681 ccgtaagcct gaaattcaca ccagataatc aatatgctga tggcgtacca gctcattgat
     1741 ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg cagtagaacg tttttagcga
     1801 tattttcagt ttctccagaa tatcttcagt tgtcatttga ttcgcaatgc agctcagaac
     1861 ctgatgttgc ttgcgcgaaa gtgacacatt cgcttcaggc acagagcagt aactgccatt
     1921 gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga acaaaggtca ccggtacttt
     1981 ccccagacag cgtttaatat taaaggggga gaaaacaatg acatggacag gttttaatcg
     2041 ttcaatttga gtgagaaaat tagaatgaaa tacattattc attaatgtat caataatgac
     2101 gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt
     2161 acctttactc agaatgtttt tcatcgcggt gaagaacatc gtatccctgg taattataag
     2221 aaacataacc gactcctggt gcggacatta actttataat cagtttgcca aaggtaatta
     2281 acaggacagg gcttataata ataatagtta gcttactgta tattaatttt atgtaataca
     2341 tgatgcagat gctgagaaag catctgcatc tttcggtggt gttatttgac cagctcaggt
     2401 gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc cggccagaat
     2461 gcaccatagc cgtaatccca caggttatag gtggtattaa ccacttctct caaatgccac
     2521 acctggatgc cctgcatatt cacttccagg aagttatacg ggactttatt gataaagcct
     2581 ggttgttcac cctgaatggt accgaggatg gtaacaaagt gattacgata gttcactgga
     2641 tcaaggaagc cgctttgtct ggcgagcagt cggccctgat agttggcttc aatatcaggc
     2701 ttcgcatagc tatccaacgg taatacagcg atttctaaca acgtatccgt tttgccattg
     2761 ataacgttga taaccttccc accaaagcgc gcttgttgac caacatataa ccccggctgg
     2821 ttatgaacag caacaaaact tttttgaata tcaggttggt tattgccttt gatattttgc
     2881 ggaattgaac tacatgcggc aagcaaaaat gaaaggctga ggatgagtgc accttttgtc
     2941 atgttcatgt tactatcctt atcaacaaat attaaagcca taatagatat ttctttatag
     3001 ttttcatctg attctgaggt gatatgcctg atagtaacgt aaacctgtct gatgtttata
     3061 tcccttttgt ttagttcaat tccattgatt ttggggcgtg gcatgagtat ccatcgcccc
     3121 ataatataat cctttgtttt aaaattacat aaaatctatt tttattcaa
//
LOCUS       DAZUFB010000242         3100 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZUFB010000242 DAZUFB010000000
VERSION     DAZUFB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.246"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1330)
                     /gene="rfbK"
                     /locus_tag="ACHY3M_005150"
                     /gene_synonym="manB"
     CDS             complement(<1..1330)
                     /gene="rfbK"
                     /locus_tag="ACHY3M_005150"
                     /gene_synonym="manB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O111 family phosphomannomutase RfbK"
                     /protein_id="HFS4319856.1"
                     /translation="MNKITCFKAYDIRGRLGAELNDEIAYRIGRAYGEFFKPQTVVVG
                     GDARLTSESLKKSLSNGLCDAGVNVLDLGMCGTEEIYFSTWYLGIDGGIEVTASHNPI
                     DYNGMKLVTKGARPISSDTGLKDIQQLVESNNFEELNLEKKGNITKYSTRDAYINHLM
                     GYANLQKIKKIKIVVNSGNGAAGPVIDAIEECFLRNNIPIQFVKINNTPDGNFPHGIP
                     NPLLPECREDTSSAVIRHSADFGIAFDGDFDRCFFFDENGQFIEGYYIVGLLAEVFLG
                     KYPNAKIIHDPRLIWNTIDIVESHGGIPIMTKTGHAYIKQRMREEDAVYGGEMSAHHY
                     FKDFAYCDSGMIPWILICELLSLTNKKLGELVCGCINDWPASGEINCTLDNPQNEIDK
                     LFNRYKDSALAVDYTDGLTMEFSDWRFNVRCSNTEPVVRLNVESRNNAILM"
     gene            complement(1336..2787)
                     /locus_tag="ACHY3M_005151"
     CDS             complement(1336..2787)
                     /locus_tag="ACHY3M_005151"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFS4319857.1"
                     /translation="MSQCLYPVIIAGGTGSRLWPLSRVLYPKQFLNLVGDSTMLQTTI
                     TRLDGIECENPIVICNEDHRFIVAEQLRQIGKLTKNIILEPKGRNTAPAIALAAFIAQ
                     KNNPNDDPLLLVLAADHSINNEKAFRESIIKAMPYATSGKLVTFGIIPDTANTGYGYI
                     KRSSSADPNKEFPAYNVAEFVEKPDVKTAQEYISSGNYYWNSGMFLFRASKYLDELRK
                     FRPDIYHSCECATATANIDMDFVRINEAEFINCPEESIDYAVMEKTKDAVVLPIDIGW
                     NDVGSWSSLWDISQKDCHGNVCHGDVLNHDGENSFIYSESSLVATVGVSNLVIVQTKD
                     AVLVADRDKVQNVKNIVDDLKKRKRAEYYMHRAVFRPWGKFDAIDQGDRYRVKKIIVK
                     PGEGLDLRMHHHRAEHWIVVSGTAKVSLGSEVKLLVSNESIYIPQGAKYSLENPGVIP
                     LHLIEVSSGDYLESDDIVRFTDRYNSKQFLKRD"
     gene            complement(2821..>3100)
                     /gene="wbdI"
                     /locus_tag="ACHY3M_005152"
     CDS             complement(2821..>3100)
                     /gene="wbdI"
                     /locus_tag="ACHY3M_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-sugar hydrolase WbdI"
                     /protein_id="HFS4319858.1"
                     /translation="IKNAFKRISSMELGKEYGISGSVFNGVWEHFYDDGFFSEGEATH
                     YIVLCYTLKVLKSELNLPDDQHREYLWLTKHQINAKQDVHNYSKNYFL"
BASE COUNT          954 a          656 c          459 g         1031 t
ORIGIN      
        1 gcataagaat agcattattc ctagattcta cattcaatcg tactacaggt tctgtatttg
       61 agcatctaac attaaaacgc caatcagaga actccatagt taatccatca gtgtaatcaa
      121 cagctaaggc actatctttg taacgattaa ataatttatc tatttcattt tgcggattgt
      181 ctagtgtaca gtttatttct ccacttgccg gccagtcgtt tatacaacca caaaccagtt
      241 cacctaattt tttatttgtc agactcaaaa gttcacaaat taaaatccaa ggaatcattc
      301 cactatcgca gtatgcaaaa tctttaaaat aatgatgcgc actcatttcg ccgccatata
      361 cggcatcctc ttcacgcatt ctttgcttaa tgtaagcatg accggtttta gtcattatag
      421 gtataccacc atgactttct acgatatcaa tagtattcca tataaggcga ggatcatgaa
      481 tgatttttgc gtttggatat ttccctaaaa aaacttccgc taataaacca acaatgtagt
      541 atccttcaat aaattgtcca ttttcatcaa agaaaaaaca cctatcaaaa tcaccatcaa
      601 atgcaatacc aaaatcagca ctatgtctta taaccgcact gctggtatct tctctgcact
      661 caggtagtaa tggattaggg ataccatgtg gaaaattacc atcgggtgta ttatttattt
      721 ttacaaactg aatcggaata ttgttccgta aaaagcattc ctcaatagca tcaataacag
      781 gaccagctgc accattccca gaattcacaa ctattttgat tttttttatt ttttgcagat
      841 tagcatagcc catcaaatga tttatgtagg catctcgggt ggaatatttg gtaatattcc
      901 cttttttttc taggttgagc tcttcaaaat tattactctc tactaattgt tgtatatctt
      961 tgagacctgt gtcactgctg attggtcgag cacctttggt tactaatttc attccattat
     1021 aatcaattgg attatggctt gcagttacct cgattccacc atcaattcct aaataccaag
     1081 tggaaaaata tatctcttca gtaccacaca ttccaagatc taagacattt acgcctgcat
     1141 cacatagccc atttgagagt gatttcttta aactctcact tgttaagcga gcatctcctc
     1201 ccacaactac agtttgaggt ttaaaaaact caccataagc gcgaccaatt ctatatgcta
     1261 tttcatcatt caattcagca ccaagacgcc cacgtatatc atatgctttg aagcaagtta
     1321 ttttattcat atttatcaat ctcgctttag gaattgttta ctgttatatc tgtcagtaaa
     1381 acgcactata tcatctgatt caaggtaatc accagaactt acttcaatta gatgcaaagg
     1441 tattacgcct ggattctcaa gactatattt tgctccctga gggatatata tagactcatt
     1501 agaaactaat agtttaactt cactacctag tgaaacttta gcagtaccgg atacaacaat
     1561 ccaatgctct gccctatgat gatgcatcct taaatctaac ccttctcctg gtttaactat
     1621 tatttttttt actctatatc tatcgccttg gtctattgca tcgaatttac cccaagggcg
     1681 aaaaactgca cgatgcatgt agtattcagc acgttttctc ttttttagat cgtcaactat
     1741 gtttttaaca ttttggactt tatcacggtc cgcaaccagt acagcatcct tggtttggac
     1801 aattactaaa ttacttactc cgactgtcgc aaccagactt gactcagagt aaataaaact
     1861 attttctcca tcatgattga gcacatcccc atggcacaca ttaccatggc aatccttttg
     1921 gcttatatcc caaagtgatg accaagaacc cacgtcattc cagccaatat ctatcggaag
     1981 aactacagcg tcttttgttt tttccatcac agcataatcg atagactctt caggacaatt
     2041 aataaactca gcctcgttaa ttcggacaaa gtccatatct atatttgctg tagcggttgc
     2101 acattcacag ctatgataaa tatctggtct aaatttccgt agttcatcaa gatatttact
     2161 ggcgcgaaat aaaaacattc cgctattcca gtaataattc ccactcgaaa tatattcctg
     2221 tgctgtttta acatctggtt tttctacaaa ctccgcaaca ttatatgctg ggaattcttt
     2281 attaggatca gctgaagaac ttctcttaat atatccataa ccagtatttg ccgtgtccgg
     2341 aataattcca aatgttacta acttcccaga agttgcatac ggcatagctt ttattattga
     2401 ctctcgaaat gctttttcat tatttataga gtggtctgcc gcaagtacta ataataaagg
     2461 gtcgtcatta ggattattct tctgagcgat aaaagcagct aaagctatgg caggtgcagt
     2521 attacggcct ttcggctcaa gtataatatt cttggttagc ttaccaatct gtcgtaattg
     2581 ctctgctaca ataaatcggt gatcttcatt gcagataaca attggatttt cgcattcgat
     2641 gccatccaaa cgcgtaattg ttgtttgcaa cattgtagaa tccccaacta aatttaaaaa
     2701 ttgtttaggg tataagactc gagacaacgg ccatagacgg cttccggttc ctccggcaat
     2761 aattacaggg taaagacatt gagacataca attctctcgc atattaattt ttaataaaaa
     2821 ttacaaaaaa taattttttg aatagttatg aacatcttgt ttagcattta tttggtgttt
     2881 agttagccaa aggtattcac gatgttgatc atctgggaga ttcaattcac ttttaagaac
     2941 tttcagtgtg taacaaagca ctatataatg tgttgcctcg ccttcagaaa aaaaaccatc
     3001 atcatagaaa tgttcccata caccattaaa aacacttcct gaaataccat actctttacc
     3061 taattccata gatgatattc ttttaaaagc atttttaata
//
LOCUS       DAZUFB010000243         3028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZUFB010000243 DAZUFB010000000
VERSION     DAZUFB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.247"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(166..242)
                     /locus_tag="ACHY3M_005153"
     tRNA            complement(166..242)
                     /locus_tag="ACHY3M_005153"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Asp,seq:gtc)
     gene            complement(375..1106)
                     /gene="dnaQ"
                     /locus_tag="ACHY3M_005154"
     CDS             complement(375..1106)
                     /gene="dnaQ"
                     /locus_tag="ACHY3M_005154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFS4319859.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1171..1638
                     /gene="rnhA"
                     /locus_tag="ACHY3M_005155"
     CDS             1171..1638
                     /gene="rnhA"
                     /locus_tag="ACHY3M_005155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFS4319860.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1635..2357)
                     /locus_tag="ACHY3M_005156"
     CDS             complement(1635..2357)
                     /locus_tag="ACHY3M_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4319861.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2391..>3028
                     /gene="gloB"
                     /locus_tag="ACHY3M_005157"
     CDS             2391..>3028
                     /gene="gloB"
                     /locus_tag="ACHY3M_005157"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFS4319862.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQ"
BASE COUNT          831 a          724 c          703 g          770 t
ORIGIN      
        1 attcgatagt atgattaaat attaatgata tggtctacca tatgtgatta acacatttac
       61 agcataacaa aaccatagac cgaagaaata gcgggattta aatgcaaaaa aaaccgcaaa
      121 attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg gaacggacgg
      181 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
      241 ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac
      301 ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct atttttagcg
      361 ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc caccagatca
      421 agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt
      481 gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt ctctccttcc
      541 atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc
      601 tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc gtagcgggca
      661 cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac cgcaaggcta
      721 tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg
      781 tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata
      841 tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg
      901 gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca
      961 tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc
     1021 ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg
     1081 atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag
     1141 ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat
     1201 ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga
     1261 cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat ggagttgatg
     1321 gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac
     1381 agccagtatg tccgccaagg tatcacccag tggatccata actggaaaaa acgtggctgg
     1441 aaaacagcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca
     1501 ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac
     1561 gaacgctgtg atgaactggc ccgtgctgcg gcaatgaatc ccacactgga agatacaggc
     1621 tatcaagtcg aagtttaagc ctgtggttta cgatattgcc gggtggctcc aaccgcctgg
     1681 cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg aatagtccgt
     1741 ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgtgcatt caatagtttt
     1801 ccaccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac ttcaaaattc
     1861 aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt atagggcgag
     1921 gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg attgaagcca
     1981 ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa taaacgatgc
     2041 ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca
     2101 aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc agaaacattc
     2161 acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta
     2221 agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg
     2281 cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac agtttgaggg
     2341 actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct atgaatctta
     2401 acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct
     2461 gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact
     2521 ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc ggcgtaaaag
     2581 aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg
     2641 gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg
     2701 taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat
     2761 tttgcggcga cacgctgttt tctggtgggt gtggtaggtt gtttgaaggg acagcatcac
     2821 aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg gtatgttgtg
     2881 ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt
     2941 ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac
     3001 ccgtaattct gaaaaatgag cggcaaat
//
LOCUS       DAZUFB010000244         2997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZUFB010000244 DAZUFB010000000
VERSION     DAZUFB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.248"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..541)
                     /gene="ybfC"
                     /locus_tag="ACHY3M_005158"
     CDS             complement(<1..541)
                     /gene="ybfC"
                     /locus_tag="ACHY3M_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFS4319863.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIAEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQ"
     gene            complement(538..2058)
                     /locus_tag="ACHY3M_005159"
                     /pseudo
     CDS             complement(538..2058)
                     /locus_tag="ACHY3M_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(2089..2415)
                     /gene="ybfB"
                     /locus_tag="ACHY3M_005160"
     CDS             complement(2089..2415)
                     /gene="ybfB"
                     /locus_tag="ACHY3M_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFS4319864.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(2415..>2997)
                     /locus_tag="ACHY3M_005161"
     CDS             complement(2415..>2997)
                     /locus_tag="ACHY3M_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS4319865.1"
                     /translation="NRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLE
                     TLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLW
                     GPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKN
                     CQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          735 a          753 c          641 g          868 t
ORIGIN      
        1 gctgtatctt tattaaacat tcgtaagact catttttgtt cgggagatgt gctgtcattc
       61 gagcataagt tggtttctca tctttaaaat attttaccat gattgaatta tcaaggactg
      121 ccaacaactg aactggatca ctgctctttt tttgatagat acccgctctt atataacttc
      181 taaaccgatc gccgagaaac gccgtttttt ctgctgtatt tatcaactcc tgcaacgttc
      241 ctttgatggc atgattttca ttaaggcgta gtgacccgac gcgaattaag agtctcgccc
      301 cgcttggtgt gaatttatta tttttacatg tacccagttc tgtttgccaa taatactcag
      361 cgatgtcctc attctgatac catctccaca tggctttatt atttaagaca ggaaacgcgg
      421 aaaaaatagt acccttagcg aaaatagtaa aacaatcatg atctttagct tgtatttcgc
      481 aatcagctat aacaccaaaa ctaacaaata tcatcagcaa aaagaacaaa actcttttca
      541 ttttcttatt cctctcgatg ttctctgttt catcttagct aaaatttcac ccaaatcatc
      601 tatccctgct gttgctggcc catcgggttt attaatgtct gggaataaat agggttctgc
      661 acaagtatat cctggtggta attgagttgt tggccaattt gtctgtggca ggtaatcact
      721 tgctgtcttt gtatccatcc ctggatactg ggaatcatcc tgtggcgtac aagtcagatt
      781 tggttttacc cacttcctgc aatacagtgt gtagtcgtaa gggcttaatg ctctgaagaa
      841 tgttttgaaa acattaggag cactactggc ctggtcaata ggatatggca gtcttaattg
      901 tttcgctatg tccggcaatt tacttggatc gggcgtaggc ggtttgttgg gtctcgctcc
      961 atatccgcca ttatttaagt gtccatattt tgatgccagt cccattgaat ctatatactg
     1021 aaccggattc agcggatact gataaaaatt ccatcccccc ttcagcccaa tcggatcctg
     1081 agtgatatac ctcccctgca gcgggtcata atagcggtgg cggttgtaat acaggccgga
     1141 ctcctcatca tactgctgcc ccggcaggcg gataagctgc tgcagctgat gcgggttctc
     1201 ttcattcagc aggttgcccc attcatcgta ttctgcgcac cattctgttg ccccttccgt
     1261 gctgacaagc gccagcggca ggccgcgatg gtcgcagtgg tacaggtgga tttttcgcgc
     1321 cggcgtgtac accgggtcca tctggttttg catctgctcc accgtcaggc cgcacgatgc
     1381 cagccagcgg cggctttcct cactcacccg gtcagccagg atttcacttt ccagccggtc
     1441 gagcatctgc accagcaccg gcgggaacac cacactgcca ccgtcttcgc cgccggactg
     1501 ctgaagggta tccgccaggc tgcggcgctg cgttttcgcc tgctcaccgg tggcggtttc
     1561 aaccctgatg agtggcgtga agctccccgg ctgataaatc gtctggatgc gggttctgtc
     1621 gttctgtatt gtggtcaggc ggtcgccgtc ccagccgtac caggtcactt gcggtttccg
     1681 tgacagcgac atccagcccg tcaggtcccg ttcacgtcgc cacacccgtt ttgccaccct
     1741 gcggcccagc gggtcgtaaa gatagcggct ttcgaccagc ggctctgcat attgtgtccg
     1801 cgtgtagtgc accagccggt gctgactgtc gtaatggtac cggtgggtgc gctcatcatc
     1861 cgtgcggata accccttccg ggatgaggtc ggttttctcc gtcagcctgc cgtgacggtc
     1921 ataccggtaa agatagtgcg cgtcacgggc gatacggtta tccggccaca tgctgagggc
     1981 gctgtccggg tacagctccg ggtcgggcag gcggttacct gccgggtctg tggcatacgg
     2041 gatatcaatg cccaggttac atataataat aagagcaatt aacttaattt atttgtttat
     2101 aatgcgctta cttaatacaa accattgaat ataccctcct gacagaaaat aaagccatat
     2161 tattatcgcc ataatatact tactatcaat ccttacgcca ataatgtctg atacagtccc
     2221 tataaatatt agagcaataa agaatactat gcctgttgga aaacatatta ccatcattcc
     2281 ataagacatg agactaatta actcgctcac atcacgagtg gagtctaata gtgaaagtct
     2341 atgcatggaa aagaatagta ttaaaagcga taaagccagc catatagctc tgaataaaaa
     2401 aataatatat ttcattacat cactccggtg aatacaaatc taataaaacg tccaatgtta
     2461 tttccggagc acttcatctt acatgtttct aaagaatcat tagaccagtc ttggcagttt
     2521 ttaaatagcc ctaaatttat aggtgtgaat atatcaggca atacagaata acgcggcctt
     2581 acctctttaa acttcccttt taaacagttc tccacacaac tattatcggt ttcaacgagg
     2641 tcacaacggc cagcagcttc ttttgtatca ttacttgctt tacctggccc ccatagacca
     2701 tctccttttg attctccccg ttggtcttgg ccaccacaag tatagtcccc tttaccatct
     2761 gggacacaaa gatattgatg atagaacgga ttcccccata tatctggacc gcttctttca
     2821 ccagttccgc ccattgaatg cagtggctta atgcatttta gtgtttctaa tcctaatgga
     2881 tctatatttg agatcggatt caacggatac tgataaaaat tccatccccc cttcagccca
     2941 atcggatcct gagtgatata ccgcccctgc agcgggtcat aatagcggtg gcggttg
//
LOCUS       DAZUFB010000245         2978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZUFB010000245 DAZUFB010000000
VERSION     DAZUFB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.249"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..438)
                     /locus_tag="ACHY3M_005162"
     CDS             complement(13..438)
                     /locus_tag="ACHY3M_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4319866.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..703)
                     /locus_tag="ACHY3M_005163"
     CDS             complement(422..703)
                     /locus_tag="ACHY3M_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFS4319867.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            803..1222
                     /locus_tag="ACHY3M_005164"
     CDS             803..1222
                     /locus_tag="ACHY3M_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319868.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1488..1640
                     /locus_tag="ACHY3M_005165"
     CDS             1488..1640
                     /locus_tag="ACHY3M_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS4319869.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNSD"
     gene            1652..2290
                     /locus_tag="ACHY3M_005166"
     CDS             1652..2290
                     /locus_tag="ACHY3M_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319870.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2291..2500)
                     /locus_tag="ACHY3M_005167"
     CDS             complement(2291..2500)
                     /locus_tag="ACHY3M_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319871.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(2799..>2978)
                     /locus_tag="ACHY3M_005168"
     CDS             complement(2799..>2978)
                     /locus_tag="ACHY3M_005168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319872.1"
                     /translation="SRYARKRPLVLTVPRALSRIHRITKFTLKRAAEQPRSKTDTAKR
                     HQKIDNRGRCSGVVT"
BASE COUNT          864 a          666 c          670 g          778 t
ORIGIN      
        1 ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa ctgcatcagg
      241 ggtgtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tacagttcag gaaacgtcat acccaactca aaataagcct gggttattcc
      361 agctgctgga actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcaagctcc tagtaagtgg ttgtgttaac gttttggtat
      481 cttccagctc gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt
      541 caccattact ggctcgctca ataaggacac ataacgatgc ccctaacatt tgacctttac
      601 tcaatgcctt tcttagataa ccgatgctag taccacactc acatgcaaac atacgctgtt
      661 catctgacga aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa
      721 cacagcaaga atacccacag gtaaacaaaa gtcaataccc ttgggttgtt taccttgcgg
      781 taatcgcatc tattatttac ctatggacaa atatgaattt agacgacaac aactcatcaa
      841 aattcgtgat gagaaatgcg atggtaaagc ggttaacgtg gccagaaaga tcggtcgcga
      901 gccttcttat gtatcaagaa tgttgtaccc agaggggaaa aaaggaaaaa aacggatcgc
      961 tgatgatatg gtggagatta tagaagagtc ctttgggtta ccccggggat ggatggatgg
     1021 tatcgtttca tcatcaacga acacagcctc caattatgaa acaagggttc taacgccacg
     1081 acaacgtatt tttttagatc tcttagacga actgccagaa agtgaagcgg ataacttatt
     1141 aaaaactctt gaagagaaaa aacagtatta caatatgatc tacgaagaaa tccgtaaaaa
     1201 gaaagcacaa aacgcatcat aactcaccaa acaaccagtc accagttaag acacatcaaa
     1261 aaattaccca tgggtattta ctttttaaat acccatgggt atccttcctt tcataccaac
     1321 ccacccagtc ccacagaacg cagggcaata cttcgagtta ccaggcagtg gtcagggggt
     1381 aagtagccag cccgaggcgt aagaacatga cggcagggtt caactttaat aactatgcag
     1441 caggtttttg ttccgctacc ccggcgttaa ggggaaatga ggtcagcatg gatactatcg
     1501 atcttggcaa cagcgaatct ctggtatgcg gcgtgtttcc caaccaggac ggtacgttta
     1561 ccgcgatgac gtataccaaa agtaaaacgt ttaaaaccga agctggcgcg catcgctggt
     1621 tagcaagaaa ctccgactga tgaggttgac gatggaattt aaagatttac ctccttcaat
     1681 ccaggagatt gcagcacaca cacttcgtca tcgtctgaac gaacttgaat tggaatcggt
     1741 aacgaaaaaa gacactgata atatggctcg taatgtgcgc gatgcgttta ccggattgta
     1801 tttctgtgcg tctataaata aacacgactc agagagtgtg gcaaataaaa ttgcagaaac
     1861 gacagcgcaa aacatcaata cgaaaccaac ggaagaagaa attgatcagt ttgctcatga
     1921 tgctggttta aaaaacaaga aagaaaaatc gccatatgcg gggaacatgt ttgtttatga
     1981 caatctcatc agaattcgtg gcgaaattcc ggcggaatac ctggcaagag tccatcaggc
     2041 attgcttaaa aatttggaaa cagaattatt tgatggcaac actaacggtt tcttcatggt
     2101 atcaggcctt gataaagact gggatgcaga aaaacgctgg aatgttgcta catggttatt
     2161 cagtaacaga gccgctgcac tggaagcttc ggcatgtatt tgcggcctgt tcttaacaga
     2221 ccacaaatat aatctggatg tgtacagtta tatttacgct gaacacggtc cactctggat
     2281 tgactggtaa ttataaggaa acaccagcag ggccgcggcg accaacagaa cgattaaaat
     2341 caataatgcc attataaagg acattattta atttatcgtc gaatgctgat tctgtgagcc
     2401 tcaactctga atgagttttt aataaccctg attgcctgag ttgatttacc aggcattcaa
     2461 tctgtttttc aataagcgga tttctttttt tgtttggcat tttatcctcc attgaggttc
     2521 tgggttaaaa atggagacca acacgctgtc acgtgtggtc gtgcgccgga cacggataag
     2581 aatccggtac tgacagttta ctgaaaggat atttccctga aaagtcaggg cataacgcga
     2641 aagcgtacgg cgaagctctt tcccttagaa ggcttgtcgt tagatttctt cgaacgtgcg
     2701 cttccggttg tggcactccg cgaaatggcg cggcggtaag tatggcgggg ttattccttc
     2761 cccgttgagg acaccgggtt gtcaggttga ccatacgctt aagtgacaac cccgctgcaa
     2821 cgccctctgt tatcaatttt ctggtgacgt ttggcggtat cagttttact ccgtggctgc
     2881 tctgccgccc tttttaaagt gaattttgtg atgcggtgaa tgcggctaag cgcacgcgga
     2941 acagttaaaa caagcggtct tttacgggcg taacggga
//
LOCUS       DAZUFB010000246         2955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZUFB010000246 DAZUFB010000000
VERSION     DAZUFB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.250"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHY3M_005169"
     CDS             <1..783
                     /locus_tag="ACHY3M_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFS4319873.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARPEQSHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            790..1536
                     /locus_tag="ACHY3M_005170"
     CDS             790..1536
                     /locus_tag="ACHY3M_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4319874.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLVEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            1558..2274
                     /locus_tag="ACHY3M_005171"
     CDS             1558..2274
                     /locus_tag="ACHY3M_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4319875.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2307..2588
                     /locus_tag="ACHY3M_005172"
     CDS             2307..2588
                     /locus_tag="ACHY3M_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4319876.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2585..2812
                     /locus_tag="ACHY3M_005173"
     CDS             2585..2812
                     /locus_tag="ACHY3M_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319877.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2805..>2955
                     /locus_tag="ACHY3M_005174"
     CDS             2805..>2955
                     /locus_tag="ACHY3M_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319878.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVL"
BASE COUNT          801 a          647 c          915 g          592 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc ctgcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcgc gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggccgga gcaatcacac
      361 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg ggcagcctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatactgg atggtgtgag gcgttacgca
      601 agatacctgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
      781 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
      841 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgtt
      901 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
      961 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     1021 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     1081 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     1141 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     1201 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     1261 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     1321 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     1381 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gcacgcttct tggtgagagg
     1441 attatggacc gcatgaccat gaacggtggt cggtgggtga cgtttaactg ggatagctgg
     1501 cgtccaaatg tcagcaatca gagggttgtg aagtaatttt tgttggagga cgttttaatg
     1561 gaaactgtat ttgacgcact gaaagcactg aaaaaagcct cttcacaggt agtggcatcg
     1621 cgccttggaa tcagccgcga agatgctgtc aacgaactgt ggaaactgaa gcgccgtggt
     1681 gaagcggata acaagggttc gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg
     1741 tcaccggtac cgaaagtgac agcgcaaatg ctgactgagg cgattgaaca tcatggccca
     1801 caaacggcgg atgagctggc cctgatgttc gggattacct cccgccgggc gaattcatca
     1861 ctggccatgg caatcagcaa agggcgtctg attcgcgtga atcagggcgg taaatttcgg
     1921 tactgcatac cgggcgctga tttaccggca gagccgaaag ccgcatccat agcggaaacg
     1981 gatggtaaag cctttcctca gccagcaggt gttgcgttac cagtcgggga agcggaaaca
     2041 caggaagaaa taaaaacgga aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc
     2101 agaaagcatc cggatggtct gattttacca tcgctgcatg tggctaaccg cgagctgcgc
     2161 cgggcaaaag gtcaggttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
     2221 aagtgccggg atattctccg ggatattacc gccaccagag aacagcagcg gtgagtgggt
     2281 ggaagacgtg gtgccgggcg gaaatcatga tactccggca gtgtgcggga acgatgaagg
     2341 taaaaagcgt tggcgcactt atcggacgaa ctgaagcggc agtgagaacg aaggcacggg
     2401 agctgggcat cagcatgatg ttacgtggtg attttcaccc gtcggcaaaa tattctcagc
     2461 gtgatattga gctggcgcgg caactgcatc agagaggcat gcaaagaagg gaaattgcca
     2521 gaaaattagg catgccgctg cgcatagtga ataactacgt ttatttcgac aggagggtgt
     2581 ctgcgtgaaa atcctgtatc aggattacgg cccggtgggg caggtggtta tcagcagtac
     2641 tgtaatggag tttcggaagc ataaccgtgt ggtggatgct gtgctgttaa cctgtccggg
     2701 gatatcggcg agtcgtgcag gtgtgtttat tatgaagacg aaattatatg gcagtaaggc
     2761 gtggataaag aaggcgtatc gtgtagcgtt gcaggaggtt aacagtgagt aaaattaaag
     2821 aaatgccggt agttcgtgac ggatatggct actggacaca tcctgaatat gaaaaattct
     2881 gtgatggtcg ggaatatatt tcaacggaag agtttaacgc ctggatggag gaaaataatc
     2941 ttcaatacgt cctct
//
LOCUS       DAZUFB010000247         2951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZUFB010000247 DAZUFB010000000
VERSION     DAZUFB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.251"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1083
                     /locus_tag="ACHY3M_005175"
     CDS             <1..1083
                     /locus_tag="ACHY3M_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFS4319879.1"
                     /translation="AINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGSRR
                     GLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGARPK
                     MGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATNDA
                     DDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLAQIVSESRRIKREKGCVGMI
                     LVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRPLP
                     SDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANGAI
                     YDTDQQSAEMRRREREEPQSKKKGGF"
     gene            1083..1352
                     /locus_tag="ACHY3M_005176"
     CDS             1083..1352
                     /locus_tag="ACHY3M_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319880.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEREMKQ"
     gene            1422..1700
                     /locus_tag="ACHY3M_005177"
     CDS             1422..1700
                     /locus_tag="ACHY3M_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319881.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            1833..2048
                     /locus_tag="ACHY3M_005178"
     CDS             1833..2048
                     /locus_tag="ACHY3M_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319882.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDFK"
     gene            2059..2295
                     /locus_tag="ACHY3M_005179"
     CDS             2059..2295
                     /locus_tag="ACHY3M_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFS4319883.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            2252..2698
                     /locus_tag="ACHY3M_005180"
     CDS             2252..2698
                     /locus_tag="ACHY3M_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFS4319884.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            2695..>2951
                     /locus_tag="ACHY3M_005181"
     CDS             2695..>2951
                     /locus_tag="ACHY3M_005181"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFS4319885.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQC"
BASE COUNT          847 a          650 c          805 g          649 t
ORIGIN      
        1 gccatcaacc gcatgactga agcgacggaa ttgctctatt cccgcaacgg aatgactgcg
       61 acgcagaagt acgaagctat tcaggcgatt ttcacgcaac tgacagacca tgcaaaaacc
      121 ggatcgcgtc gcggccttcg ctcatttggc gaggtcatgg aagactgggt tagcgacctt
      181 gagaagcgat ttgacccatc aggcgaacaa cggggaatga gcacagggat cccatcgctg
      241 gacaggatgc tgtcaccgaa aggtctggtg aaaggctctc tgtttgtcat tggcgctcgc
      301 cctaagatgg ggaaaacgac gctatacagc cagatggcaa tcaattgcgc agtgcatgag
      361 aaaaagcccg ctctgatgtt cagccttgaa atgccaggtg atcagatact ggaaaaactg
      421 gtagggcaga agtctggtgt taacccgaat attttttacc ttccggcgac aaatgacgcc
      481 gatgacggct atcagggtga ttacgatggt gacttcaaca gggcgatcga aacagccaat
      541 cgcttgagtg aaatcgacat gctttacatc gacgacacgc cgggattatc tctggctcaa
      601 atcgtcagcg aaagccgtcg aatcaagcga gaaaaaggat gtgttggcat gattctggtc
      661 gattacctga cactaatgac cgctgagaag gccgatcgca acgaccttgc ttacggcatg
      721 atcaccaaag gactgaagaa ccttgccaaa gagcttgatt gcgttgttgt gcttctgacg
      781 caacttaacc gcgcactgga aagccgaacc aataaacgcc cattaccaag tgactcacga
      841 gatacagggc agattgaaca ggattgcgat tattgggtgg ggatccatcg tgaaggtgct
      901 tttgatgaca gtgttccacc tggtgaaacc gaactaatcc ttcgtctcaa tcgtcatggc
      961 aataccggca cggtgtattg cattcaggca aatggcgcta tttatgacac agaccaacag
     1021 tctgctgaaa tgcgccgacg tgaacgcgag gaaccgcagt ccaagaagaa aggaggattc
     1081 tgatgaataa aaaacaatta gccattctcg aaaaggcatg ggatgcgcaa atatcatacg
     1141 ctttgaaaga acaggcacta ccaataatcc agaccaaatc gaaaatagcc aggcagttat
     1201 gcgatggcgg attcctgaac gaagttgaga ttacgcgcca gatggtaacg ttcaaagggt
     1261 atgagataaa tcatcatggt atagcggcgt attgctccca tcttcctgat gacgttgaca
     1321 ttgatgaaat ggaaagggag atgaagcaat gaccatctac atcactgagt taataacagg
     1381 cctgctgtaa tcgcaggcct ttttatttgg gggagaggga agtgaacgat agctaccgac
     1441 agtttgaaaa ctggtggtca aaagacaaaa gccagttcac gggagacgat gaattaaaag
     1501 agtttgcctg ggtgatatgg caggcatcgc gctctgctat tgaactggat atcgactggc
     1561 ccgaatcgaa tgacgacctt tggaaagatg gtgaagaagg tgcttatgcg atgggttatg
     1621 aggatgggcg tgacaaaacg gtaattgcag taatgaaagc catcagggcc gcaggaatca
     1681 aagaaaagaa tttcgattaa gcaaatatca cttcaataaa tcgcttttaa ggcatcacaa
     1741 tcgctctgtg gtgaggtaag cacgtgcaag gtataccgat aagcagcgag aatgaaaaat
     1801 gcgtcagaat gcgtttgagg aggttttaag aaatgagtac gatagctgag cttgtcaggg
     1861 ctaattttcg tgaagagttg gtgcgttggt atcggtatcg ttcatcgtcc agtttgccgc
     1921 ttgatgagtt gtatgagcat tcacctgccg cacgacgcta tccgcgtgac cgtgttcttc
     1981 gacggttgtt caaactcaac aatgagtttc agcgcaacag aattatccgg agtctggatt
     2041 ttaagtgaag gagtgagcat gagcgaccta tcattaaccc agccaaagct aaaagaatgt
     2101 ccgttttgcg gcggtaatgc tcgtctgtgg gttgaggccg gaataaatat tgatgtgtgg
     2161 ggctatgcag aatgtgacct ctgtgaagcc agggtggcat gggcaccatc agttgctgcg
     2221 gctactgaaa aatggaaccg gagagcagga gatgaagcaa acctttctgc ttcgcaacga
     2281 agcaatcaga aataacgcca tagacgccat tctctcacta cccatcgacg acaagtcacc
     2341 ccacgaagtc cacgttaaag aacccaagcg cagcaaagcg cagaatgacc gcatgtggcc
     2401 gatgctgaac gatgtttcgc gtcaggtgct atggcatggt caacggctgg cgccggaaga
     2461 ctggaaagac ctgttcactg ccctgtggct taagaccaaa aaactggagc aaagaagtgc
     2521 gcctggtatc gacggtggcg ttgtcatgct tggcgtgcgt accagcaaaa tgcgaaaggc
     2581 cagcatgact gagcttatcg aaatcatgtt ctggttcggc tcagagcgca acgtgcggtg
     2641 gagtgatgac tcccggcgag agtatgaatg gtcacaacga aaagggaagg ctgcatgact
     2701 atcaaatcaa atacgccagc acacgacaag gactgctggc aaacgccgct ctggcttttt
     2761 gatgcgctgg atattgagtt tggattctgg ctggattcag ctgcgagcga caaaaacgct
     2821 ctgtgcgctc actggctaac tgaggctgac gacgcgctaa attctgagtg gataagccac
     2881 ggtgcaatct ggaataaccc accgtacagc aatatcaggc cgtgggtgga aaaagccgct
     2941 gagcagtgca t
//
LOCUS       DAZUFB010000248         2802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZUFB010000248 DAZUFB010000000
VERSION     DAZUFB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.252"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..1360)
                     /gene="pilV"
                     /locus_tag="ACHY3M_005182"
                     /pseudo
     CDS             complement(62..1360)
                     /gene="pilV"
                     /locus_tag="ACHY3M_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
     gene            1375..2571
                     /locus_tag="ACHY3M_005183"
                     /pseudo
     CDS             1375..2571
                     /locus_tag="ACHY3M_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
BASE COUNT          844 a          663 c          563 g          732 t
ORIGIN      
        1 aggatagaat actcctttaa tctcgccata cttgtcaaat attaattttc agtaagtagc
       61 attacctaca tgcatataac cttctgaaat tactataaca atgtgtattc ctaccaccac
      121 tacaacgctc ctcattatat tctgcaataa gggttccaga tatataacca aactgaggac
      181 tatacgttga tcccggacag gcacatgttc cagtatcact attcggtata acgcatttat
      241 aaccagagat ttgatctgtt atcgttataa aaataccttt caggccatca ccacttgctg
      301 tctgccattt accgttaaca cacgataaca ctcgacctgt actgtctcgc cccaccagac
      361 cattaggcgt acatttcgca tttaatgttg cctgaacatt caaatggaga tattcacctg
      421 ctttgatcct gcctgttgat tcaattgtcc cgttagatat tatcgtgcct gcggtaagat
      481 tacctgaagc ataaatatta cctgcattgt tcatgtaaac tttaactgtt ttcccggttc
      541 ctacaccaac agtccctgac gcatacaaat cataggtgcg aacaccgcca gcccagccag
      601 caggcaaatc gtttggatta agtccgttcg tcgcgatacg actaccaaac ttcgccagac
      661 ctgttgttga tagcgtatta agataggtat cagattttac tttcaggttg ttgttgatat
      721 ttacaggacc attaagggtt gaggttcctc ccacagtcag agctgatgta acagacgcat
      781 tacccgttgt cttaagtgcg ccggttgttg ttgtccctga tgccgtaatg tctttgatgt
      841 ttttgatact cttcccacca agattcagat catccgtcat cggcaacgtt ccatcacggc
      901 gcagataaac agaatacatt gaagagttat accccacgcg gtaagccagc aatccaccac
      961 ccgtgatatt gctgtagcta ttagagttct cactccactg accaccatag ccggacgcta
     1021 tcttggtgtt tctgctcatc ccaccatcaa caccagccgc ctgaaccgct ttacctaaca
     1081 ggtcataacg aatacgacca ccttcttccc acgggctggc ggttgttata agaccattga
     1141 tgacataatc aggtgctgtt cctgaacgct ttaacacaat cttgtaggat gctttttgcg
     1201 cgttagttcc tgtatagccg acaggcaaca gaccttcatt aattaacgtc tggtaagtga
     1261 tttcacaacc agcagccgaa catgttcttg gccccggatc gctactttga ttgttggatg
     1321 atgacagtgt ggaaattttg tcatagcgaa tactgatata gtgaaccgcc ccgggtcgct
     1381 atccccgtag aactcagcca tgatgacaag aaaaaattgg ttgccgaata ctgccagaag
     1441 aactttgttg ataaaggaat gattgctgat atcgcattcc acgatttaga cagtaagaat
     1501 ccacatgctc acgtcatgtt aactctaaaa actattggtc ccgaaggctt cggtaaaaaa
     1561 gacagaagct ggaatgataa gaagataatg atccaatggc gtgagtcatg ggccacaatg
     1621 tcaaacagct accttgaagc cgcaggcagt gaagaacgta tcgatcaccg ttctctcagg
     1681 acgcaatgtg ctgatgccct tgcccaagca gaagaagcat tcagcgctga agagaaagca
     1741 ttctggcttg ccaaagcaac ggaaacaaac cgcccggcaa tgcaacgtgt tcatcgtgct
     1801 aaatggaatg acaaagagtc tcaggaacag cgagctactg aacaagctca acgcgatcat
     1861 caaattgagg aagcaaagaa ggtctacacc acattcagtg agctaccact ggaaatcgtt
     1921 gttgatgtca gaagctttac gattacacat cttgctgacc ccgaagagat tgttcttcca
     1981 gactacccgg caacagtaga acagcagtcg attgtggcaa cagcaactac aagcagaaga
     2041 ccacctgtaa aatcataccg tgatcccaat aaggtcagta aagtaaatgt ttcagggaag
     2101 aaatcccctg ttctggttgc gcctgaacca actgcatcca ctaaattgaa aaccccatat
     2161 cttcaaaaca ccagagcgat gaaccgcgct cctgtgagga gtaggaaaca gaccaagccc
     2221 cgccagaatg gtttgtttaa gcgtttcaca ctactcatcg tggacttctt gaaagagaga
     2281 tttgtatggg ccaaaaggaa acccgatacc actgatgctg accatgataa gcgtatcgca
     2341 gagaactatg tctatgatga agtgttgggt gtcaatgttc cgcgctctga gtttgaaaaa
     2401 cgtaccaaat ttaacaatgg tgactataaa ttaacatctg atgagatcaa gcgtttccca
     2461 agccgtccta atcaggaaca gccggaagta aatagaagct tagatctcac accttcaatg
     2521 ccaccagaac acatgaggca aggaagcgcg ccaaaactga gtccaccgta atacatgact
     2581 cctataatgc tgtagaataa tagttaatgc tgttcatttc ttatcatgat aaaaaagaga
     2641 taaaattata tggaaatcaa tgagtggttt gacaaaataa acaaccttcc attagagaaa
     2701 agcaaatggt gttccttggg gactttttat cgagcattca ttgaacggtc gaacttactc
     2761 actgatgagt tattccccca cataagtatc tcattagaaa ag
//
LOCUS       DAZUFB010000249         2773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZUFB010000249 DAZUFB010000000
VERSION     DAZUFB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.253"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..539)
                     /locus_tag="ACHY3M_005184"
     CDS             complement(<1..539)
                     /locus_tag="ACHY3M_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4319886.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSE"
     gene            complement(550..816)
                     /locus_tag="ACHY3M_005185"
     CDS             complement(550..816)
                     /locus_tag="ACHY3M_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319887.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            973..1584
                     /locus_tag="ACHY3M_005186"
     CDS             973..1584
                     /locus_tag="ACHY3M_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4319888.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            2002..2529
                     /gene="sodC"
                     /locus_tag="ACHY3M_005187"
     CDS             2002..2529
                     /gene="sodC"
                     /locus_tag="ACHY3M_005187"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS4319889.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          741 a          664 c          621 g          747 t
ORIGIN      
        1 atttcactca acgcacggct gtttcccttt ttggcgttcg ccgtctcaac cattgccaca
       61 atgcgctcgt ccagttgcga acgtgccagg ttgtcaacga taaaccggat agctttcgca
      121 cagctgaaac cgggttgttg cgcgactttc atcacttcgc tgacgatcgc aattcgtgca
      181 tcagccacct ggcgctggtt ttcagtcagg gcattaaggc gttcaaccat cagttgtggt
      241 gatccgcgat atgcctccac cgcatcaacc acggcaggcg agcgtctggc ctttgtcacc
      301 accggagccg gtgattcatc agctttttgc gtcatcagtt gaagggcata acgttcacgc
      361 aatacttgtt gcgcgccaga aggcaaacaa tcgatattaa attcgattgc ttttgtcccc
      421 ttacgtttgc gtaacatggc tgcatttccc gcagctgctt ttgtgagtcg atggcgaatg
      481 ttatgttcca tcattggtaa atcaggaaga ccaacacatt cttttgctgt tacccacata
      541 catcccccat caagccactt ctctggcgtc cacctggtat ctgctgggcc agattacagc
      601 aggttcaacc cctatttttt cggcgatggc ggcttcgtat ttacggcact ggcggtaaaa
      661 cacattgcgc attgatccgc tttttaatcc taactccctt tccagttcag ggaggtctgt
      721 gcctttgttt tcaagggctg catataccgc tttagcagac cagtcacacc cctctcgaac
      781 aaacaaatta ttcgatgctt gtctggatac cctcattgtg tgatcctctc atgtttacct
      841 gaaccgataa tcaaaaccgg ttatcgattt aggtaaatca taatcgcaaa ataattcgat
      901 atcaacggtt ttttgcgagc atatcgaaat taaatgcgat gtcgataatt tctttgtttt
      961 atagggggtt atatggataa ggcagaaaaa accgtatcca gtgcggaacg gtttattgat
     1021 atggaaggaa taaaccgatt ttcagaacgc ttagctatgg ctatgggaaa catgacaaac
     1081 gtcgaactgg cgaccctttg tggactatct gaagccacag taagaaatta caggaagggg
     1141 aaaaacttcc ctcctttgga taaactggcc ttaatagcta aagcctgtaa ttgttcactc
     1201 gaatggttgg ccacaggcaa gaacgaagat gaatgcggaa aaatcgaaaa caattgcgat
     1261 tcaggttcgg gtattgatca ggcgcttagc tctctaagcc cccaagagaa gcaattaatc
     1321 cttacattca tcaggcgaga aggtactaac aaccttgtca ggctggcagc aatgaataca
     1381 tctacaatca gccaggatgc ggtagaaagc atcattgatg ccttgccatt acgtcccgta
     1441 ctaaaaaatg caataaagat aggaatggcg ggaagtgaag aaacagacaa agagattttg
     1501 cgcgttattg agaagtacaa ctcgagcaat aacgccaccc aggaatcagg ggcagtgaca
     1561 gtaaagaaaa atgttgttgg ttaagcgcca tcgctatcat gaggtggtgc tgtaaataca
     1621 tatagagatc atatgactca tcccaagata atcacgctat tgaatagtaa ttcatgatga
     1681 ttatcatact gattatagtt agatcgatta tgtgagcatt ttggcagttt gaagatgttt
     1741 tgaagatgtt ttgaagaaga ttttaagggt aaattagcag tatcaaatgt gtcgccgttt
     1801 tttgatcttt tgactatttg ttgagcagtc ggtatcaaaa agagttttcc gtccgaagta
     1861 tacatcacct gttttatttc agcatcttac aggtgatttc acctcctttc actaaaaccc
     1921 ttgtcggtat caaatgattc acctggttac ataccctgta accagcaagc tcagtctgtt
     1981 aacggaatta atgagggttt tatgaaatgt aaaatcattg ctgccattgc catgctgaca
     2041 gcagcatcat gcggatacgc agcagaacag gaagtcccaa tgaaccttgt cagtgctgac
     2101 ggaaaagaag tcagcattgg aaaaataacc attcaggaga ccccctacgg tctgctgttc
     2161 acaccagccc ttcactctct gtctgaaggc attcatggtt ttcatgtgca cgaaaaagga
     2221 aattgcgccc cggcactgaa agacggaaaa ccggtcgcag cattatcggc tggcggtcac
     2281 tttgacccga aaaacaccgg caaacatctt ggcccctggt ctccggatgg acacctgggc
     2341 gacctccctg cgctgttcgt gacgcatgac ggaaaagcga actacccggt cctggccccg
     2401 agactgaact cattaaaaga gattaaaggg cgttctctca tgcttcatgc tggcggtgat
     2461 aaccatcatg accatccgga gcccctgggc ggtggtggtg cgagaatggc ctgcggcatc
     2521 attcaataat cagtcaggta aggggcgggc cccttacctt tattcctcag gacgataaat
     2581 cctttctccc tgaaaagaac ggcacatcct ccctctctga gttaatgttt ttgtcgtgac
     2641 ataagaataa ttccttacac tcaatcttcg taacgctccc gcagttcctg tccgtgagca
     2701 ctgcgggatt ttttcgcttt tatgcctgcc gcccgataac cacgaccttt ccgcccccgc
     2761 cttcatcacg ggt
//
LOCUS       DAZUFB010000250         2771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZUFB010000250 DAZUFB010000000
VERSION     DAZUFB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.254"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..485
                     /locus_tag="ACHY3M_005188"
     CDS             <1..485
                     /locus_tag="ACHY3M_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4319890.1"
                     /translation="PDFNYIYQNYGDDFVTHTAKFSGETVERAIERAIVSKLNIVVEG
                     TFRNAATPLQTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAGNIARKVDKN
                     HHDIVTGLLAENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLIQNEISGNTQ
                     "
     gene            complement(521..1312)
                     /locus_tag="ACHY3M_005189"
     CDS             complement(521..1312)
                     /locus_tag="ACHY3M_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4319891.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1318..2445)
                     /locus_tag="ACHY3M_005190"
     CDS             complement(1318..2445)
                     /locus_tag="ACHY3M_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFS4319892.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMVN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            complement(2569..>2771)
                     /locus_tag="ACHY3M_005191"
     CDS             complement(2569..>2771)
                     /locus_tag="ACHY3M_005191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319893.1"
                     /translation="SQFWHASRNISYALKLNYKGGQTPSAHQERPGCFPGVFRQPLTP
                     IMGSIPTFIPEMNTGFNLLNKK"
BASE COUNT          714 a          761 c          605 g          691 t
ORIGIN      
        1 accctgattt taattatatc taccaaaatt atggggatga ttttgttacc catacagcga
       61 aattttccgg cgaaacggtc gaacgtgcaa ttgagagagc gatagtcagt aaactcaaca
      121 ttgttgtcga aggaacattt cgtaatgccg cgacgccgtt acaaacatta aaaaaactca
      181 aagatgcagg ctaccaaaca gaagtcatga taaaaacaac ctcagcggca ttaagctggg
      241 aaagcaccaa tgaacggtat agtaaggaca aagaggcggg aaatattgcc cgaaaagtgg
      301 ataaaaacca ccatgatatc gtcaccggtt tgctggcaga aaatgcccgc aaggtgtttg
      361 cttcgaacct gtcagataag tttgcggtgt attcgcgtga gaagatgata tttagttcac
      421 aagcggcaac gaatgacgat attgcaacgc ttatacagaa tgaaatcagc gggaatacac
      481 aataataata agagctgatg gcgtgaaata acgccgggct ttatgcccgg cgcattaccg
      541 tctggtacat taagctatcc agttccttaa cgtccccttc tctgctatcc ggtaacagcg
      601 gtggaatttc cccacgcgcc agttccgcac gaataaacgc atgtaacaat ggacggcgcg
      661 gaccatattc cgcttctcct gcgcgctgtt tacgttccag taattcgtct atttctgcac
      721 gtaacggcgc atcaagttcg cttcccgcca gtaactcagc aaagcgcatt ggcggtacgc
      781 ctttacccgc ttcgacccag cgcacggcca gcagcggacg cagcacgtaa aagtattttt
      841 tcagtcgaac ttcgtcgccc tgcaaatatc cggggaagtt tttctgcgcc atcgaatagt
      901 aatgccagcg cgcacgcagc ggagaaaacc acattgggac cattgcctta aacgccgtta
      961 tcgtttcttc atcttgttga taaaccaccg gagaatccag ccattctatc agcgtcgggt
     1021 tcgcgccttt caataatccc agcgccttac gccactccca accggaaaca tctaactcgt
     1081 cgtcgatggg cagctcaata acatcgcgcg gtgactctac tcgcagatac cattccagcg
     1141 ggtgaacata caaaaaccgt acatcgtagt cgctgtcggg cgaggcaaat ccccagccgc
     1201 ggctccctga ttcacaggcg tacagtactt tcacatcata ccgccgttca atttctttta
     1261 actgctgcga taccctttct cgcatcgcag cactgactcc atttaatggc atgttactca
     1321 ccctttgata ttaaccaacc catgcagaac ctgatccaga ccgccaaata cagagatgtt
     1381 atcgatccgt tcagccactc tctccagcgt ttccagttct tttaagcgta acgctaccgg
     1441 gttgtttacc atgactttag cggtattgag taacgaacgc gtcgctgccg tttcttcacg
     1501 tcggcgaatc acattggcct gcgcggattt ctccgcctca accagctgcg caaggatatt
     1561 tttcatatcg ccaggcagga cgatatcttt cacccctaac gaggcaatct ccataccaaa
     1621 tggcaacatg cgcgatttca cctgttcgct gacaacgtca tcaatgacct gtttatcttc
     1681 cagcaattca tccaacgtac gcgtacctac cgcttcacgc agggcaaatt gtaattcccg
     1741 atacaaatga tcaatcggct tcgttaactg gctaaatgcc aataacacgt cgctgtagcg
     1801 ccagttagcg gcgaggttaa tccgcagatt gaccttatct ttcgttaaga tttcttgccc
     1861 gctgacttcc agtacctgca agcgggtgtc aaccacttca gcgtcaacca gatggttaat
     1921 tttccagtat gcggtcagcc ccggcggtaa cagcgcctgc gtttcaccat cgattttcag
     1981 cacgcctaca tgccaggccg gaacctgcac cgttagtatt gcatcgcggc ctttaactgc
     2041 ccctttgcgc cgtggttgca ataccgcatt catcacctcc gtttgtacct ggacttgctg
     2101 cgtattcatc cgcaccagtg tcaaatcatc ctctacacgc cagtacaaac ggcgcgttga
     2161 tggcggcagg atctcctgca aaataccgtc catatacaac gccccggctt caatctccga
     2221 caagtctgcc accaggcaat atttctccac ccaatcaggc tgaaagcggc gcaaataatc
     2281 cgccagcacg tcgggaactt cgctgccatc gagcgtaatc agcaagactt ctggcgtatt
     2341 taaccagggt aaaagatgct cgcctgcatg tagcactttg tagtaatcgc cgtttctgga
     2401 gagtaatgcc agctgatctt ttcttacgct tattttttta atcattatct attccttgct
     2461 taacagccgt cgcggaagca gacacgaagc gacaggggaa ttcccttttg cctgttaccc
     2521 ggcatcttta tcggcgacgt tatcggggat cgctgactgc gcaatccctc actttttatt
     2581 taataagtta aaaccagtat tcatttcagg aatgaatgtg ggaatcgaac ccataattgg
     2641 cgtcaaaggc tgacggaaca cgcccggaaa acagcctggc cgttcctggt gtgccgacgg
     2701 ggtttgaccc cctttgtaat tcagtttcaa cgcgtacgag atattgcgag aagcgtgcca
     2761 gaattgagaa a
//
LOCUS       DAZUFB010000251         2749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZUFB010000251 DAZUFB010000000
VERSION     DAZUFB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.255"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..1008
                     /gene="ompT"
                     /locus_tag="ACHY3M_005192"
     CDS             55..1008
                     /gene="ompT"
                     /locus_tag="ACHY3M_005192"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFS4319894.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKLPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1195..2679
                     /locus_tag="ACHY3M_005193"
     CDS             1195..2679
                     /locus_tag="ACHY3M_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4319895.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          881 a          585 c          558 g          725 t
ORIGIN      
        1 ttagatataa aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg
       61 gcgaaacttc tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc
      121 gagactatat cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc
      181 ggaaaaacaa aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc
      241 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
      301 cagatatcta ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc
      361 gatcaggact ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct
      421 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
      481 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      541 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      601 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaactgcc ctacattggc
      661 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
      721 tgggtggaag catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc
      781 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca
      841 cctaacgcaa aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
      901 acttcacttt atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata
      961 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca
     1021 gattttctcc ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc
     1081 aatatccact aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa
     1141 cacaagacct caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga
     1201 aaaataatta ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc
     1261 cagcagatac agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa
     1321 aaaccgtctc ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca
     1381 ggcggagatg cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg
     1441 gcaaaaagca agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc
     1501 ataaatcctt atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt
     1561 attggatttg atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca
     1621 gatacagaga ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc
     1681 tgtatccata ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac
     1741 gatattgatc gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg
     1801 tatggaactc aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc
     1861 gttcttgatg gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa
     1921 caggcacaga gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa
     1981 agttgcccgc tttcttcaga cagagataag gcaataactc agttccatga gctattatca
     2041 aaattacatc acaaaccttt attagacagt aagagagaaa atatatcttc agatgaactc
     2101 atatcattaa caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc
     2161 atacgccagt tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg
     2221 atagcctcag aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat
     2281 gagcaattaa caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg
     2341 gctattaact ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata
     2401 catagcgacc tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt
     2461 atagcacaca aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa
     2521 ttttccagcc cgttactaac aataaatggt gatgggcata cattagctct caccggagtt
     2581 aatttatgtg tagataaagc agttgtacat cacctgatca ctccacaaaa aatagaaaat
     2641 atatactgcc caggaaattc tgaagcagaa atacaataat ttcaaaatat tccgctattt
     2701 gctcccaatg caaaacatat attgcattgg gagcatattc atatttttg
//
LOCUS       DAZUFB010000252         2710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZUFB010000252 DAZUFB010000000
VERSION     DAZUFB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.256"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            437..1384
                     /gene="stxA1a"
                     /locus_tag="ACHY3M_005194"
     CDS             437..1384
                     /gene="stxA1a"
                     /locus_tag="ACHY3M_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFS4319896.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1394..1663
                     /gene="stxB1a"
                     /locus_tag="ACHY3M_005195"
     CDS             1394..1663
                     /gene="stxB1a"
                     /locus_tag="ACHY3M_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFS4319897.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2068..2148
                     /locus_tag="ACHY3M_005196"
                     /pseudo
     CDS             2068..2148
                     /locus_tag="ACHY3M_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            2447..>2710
                     /locus_tag="ACHY3M_005197"
     CDS             2447..>2710
                     /locus_tag="ACHY3M_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFS4319898.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAA"
BASE COUNT          738 a          507 c          678 g          787 t
ORIGIN      
        1 gtacccagaa tcctgtgagc tatacggaaa gtacacagaa aggaaggtgc gaccacaatt
       61 aataacaaaa tcttaaaaat tgcacatggc actattagtt ttctaaatat tgtgtatttt
      121 ttgtattgca ggatgaccct gtaacgaagt ttgcgtaaca gcattttgct ctacgagttt
      181 gccagcctcc cccagtggct ggctttttta tgtccgtaac atcctgtgta tcaataaatg
      241 ttgttgtcta cgtacgtcaa gtagtcgcat gagatctgac cagatatgtt aaggttgcag
      301 ctctctttga atataattat cattttcatt acgttattgt tacgtttatc cggtgcgccg
      361 taaaacgccg tccttcaggg ggtggaggat gtcaagaata tagttatcgt atggtgctca
      421 aggagtattg tgtaatatga aaataattat ttttagagtg ctaacttttt tctttgttat
      481 cttttcagtt aatgtggttg cgaaggaatt taccttagac ttctcgactg caaagacgta
      541 tgtagattcg ctgaatgtca ttcgctctgc aataggtact ccattacaga ctatttcatc
      601 aggaggtacg tctttactga tgattgatag tggcacaggg gataatttgt ttgcagttga
      661 tgtcagaggg atagatccag aggaagggcg gtttaataat ctacggctta ttgttgaacg
      721 aaataattta tatgtgacag gatttgttaa caggacaaat aatgtttttt atcgctttgc
      781 tgatttttca catgttacct ttccaggtac aacagcggtt acattgtctg gtgacagtag
      841 ctataccacg ttacagcgtg ttgcagggat cagtcgtacg gggatgcaga taaatcgcca
      901 ttcgttgact acttcttatc tggatttaat gtcgcatagt ggaacctcac tgacgcagtc
      961 tgtggcaaga gcgatgttac ggtttgttac tgtgacagct gaagctttac gttttcggca
     1021 aatacagagg ggatttcgta caacactgga tgatctcagt gggcgttctt atgtaatgac
     1081 tgctgaagat gttgatctta cattgaactg gggaaggttg agtagtgtcc tgcctgatta
     1141 tcatggacaa gactctgttc gtgtaggaag aatttctttt ggaagcatta atgcaattct
     1201 gggaagcgtg gcattaatac tgaattgtca tcatcatgca tcgcgagttg ccagaatggc
     1261 atctgatgag tttccttcta tgtgtccggc agatggaaga gtccgtggga ttacgcacaa
     1321 taaaatattg tgggattcat ccactctggg ggcaattctg atgcgcagaa ctattagcag
     1381 ttgagggggt aaaatgaaaa aaacattatt aatagctgca tcgctttcat ttttttcagc
     1441 aagtgcgctg gcgacgcctg attgtgtaac tggaaaggtg gagtatacaa aatataatga
     1501 tgacgatacc tttacagtta aagtgggtga taaagaatta tttaccaaca gatggaatct
     1561 tcagtctctt cttctcagtg cgcaaattac ggggatgact gtaaccatta aaactaatgc
     1621 ctgtcataat ggagggggat tcagcgaagt tatttttcgt tgacttagaa tagctcagtg
     1681 aaaatagcag gcggagattc ataaatgtta aatacatctc aattcagtca gttgttgccg
     1741 gtctgataat agatgtgtta gaaaatttct gcatggtgaa tccccctgtg cggaggggcg
     1801 actggtgaac ggtatgatct ctttgatgat cgtaagcgag aatacgcggg tttggtggca
     1861 ccaggccgaa ctcaccggga ggcacccggc accatgcaat ggcacatagc gccactctcc
     1921 agcccctctc cggaggggct ttcttatgga caaaaaaagc ccgcgcaggg agacgctggc
     1981 ggcaaggaat aaacaacaaa acgtgaagta atatttcagc tggcgaataa tatccgacag
     2041 taatcactct gcgcaatagc gcggcctatt cgctcaaaca actgttacgg tcaggtatcc
     2101 cgtcgcgacc aggagagtgc gctggcgtgc tggggaattg acagataagc agaatatttt
     2161 gctgaaaaat gcggtttgct cacacggacg gataacacga aatcctgcga actgacaaaa
     2221 actaagtgaa taaaagtaaa aaccccgttt gttggctgca agcggggttt tgtgtttcct
     2281 gactctggaa aagtcaaagg agaaagtgtg tttgatttta gcaaactgat tcgggagatt
     2341 cgagtgatgg ctgaaaaatt atctacctgg aagttcattc ttatctggct ggtgtttgtg
     2401 attatggtct ccggttattt catcggtcag atacgctggt ggtgaaatga accgcgtact
     2461 gtgtgtggtc atcattgccc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca
     2521 ttaccgtgat aacgccatca cctacaaagc gcagcgcgat aaaaaagcca gagagctgga
     2581 gctggcaaac gcaaccatta ctgatatgca ggtgcgccag cgcgatgttg ctgcgctcga
     2641 tgcaaaatac tcaagggaat tagctgatgc gagagctgaa aatgaaactc tgcgtgctga
     2701 tgttgccgct
//
LOCUS       DAZUFB010000253         2674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZUFB010000253 DAZUFB010000000
VERSION     DAZUFB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.257"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..253
                     /locus_tag="ACHY3M_005198"
     CDS             35..253
                     /locus_tag="ACHY3M_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFS4319899.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            255..812
                     /locus_tag="ACHY3M_005199"
     CDS             255..812
                     /locus_tag="ACHY3M_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4319900.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1103..1258
                     /locus_tag="ACHY3M_005200"
     CDS             1103..1258
                     /locus_tag="ACHY3M_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4319901.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1378..1722
                     /locus_tag="ACHY3M_005201"
     CDS             1378..1722
                     /locus_tag="ACHY3M_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFS4319902.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1844..2116
                     /locus_tag="ACHY3M_005202"
     CDS             1844..2116
                     /locus_tag="ACHY3M_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319903.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2118..>2674
                     /locus_tag="ACHY3M_005203"
     CDS             2118..>2674
                     /locus_tag="ACHY3M_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319904.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          700 a          607 c          728 g          639 t
ORIGIN      
        1 aacatcagga ggccgcccga aagggcggta agaaatgaaa cattatttag aaaaaaatta
       61 cccacgaaag agcagaacaa cagagtttct gtttttcatt ctgtttatag tgttgatgat
      121 accgatatcc ccgctattac tggtctggat aattggaagg acatttgaac cagttattga
      181 gctatatacc gatgtgacat gggaatcatt cagcgcactg cacaataaaa ttaatccgta
      241 taaggaaaac tgatatgagc actattacca gagaacgcgc ggagattaaa tcatacatca
      301 caggcttcct gagcgactcg gcgcacgata acaagtcttc agacagcctg ctggctaatg
      361 tgtttcgtat cgcgctggca tcactggaag cagagccgat agcaatggta gtgcctgatg
      421 aaatggattt gcttacctgc catctcgacg gtgtaactaa aacatatgct gatggctgga
      481 acgcctgccg cgtcgccatg cttcaggccg gaaactttcg ggaaaataag aattcgtcaa
      541 ccaacaattt tcgggaaatc tcggaaacgt caaccagatc tccgataact ctggatggct
      601 ggataagctg tactgagcga atgcctgaaa agagccagaa cgtgcttatt tcgatgaata
      661 tcgatagcga ggctgggcca ttaatatatt ccgcacgcta tctcggaggc acgttccggc
      721 gcggaggtat agcagttagt ccgggtaatg atcttaggca agcaacccac tggatgtcgc
      781 taccagaacc gccgcaggag gtgaatcaat gacctggcct gaagcattca caacggtagg
      841 aattgcgatg gcggtggcgc tggtggagta ttcgatttgc cgctggggat aaaaacggtt
      901 tgcgggaaaa ggatagttaa gtagaattgc agcgggtgct tgaggctatc tgcctcgggc
      961 atgaacacca acggcagata gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc
     1021 ttaacattaa tctgaggctc aatccatgct gaacacatgt aggttagcct cttacgtgcc
     1081 gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt
     1141 catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct gcgaggtgcg
     1201 aatccgaacc ggtcagacgg aggtcgctgt cttcgtagac tacgaatctg agaagtaaga
     1261 gaccaggcgg gggagtaatc tcccgccacc tctgatgtgt caggcatcct caacgcaccc
     1321 gcgctttacc atactgaaaa tgctgtttga atgttcatct ctgaaagagg actatgaatg
     1381 aaaaaggtat tgattgcagc acttatttcc ggtgtgtctt ttggcgcttt tgcacagcag
     1441 ggtggtttcc aggggccaga agcagagcgt tcaacagtag cgcaggcaaa agaactgaag
     1501 gatgatgcat gggttatcct tgaagggagc atcgttaaaa aagtgggtga tgaacgttat
     1561 gagtttcgtg acaatagcgg gacaattgtc acggatattg atgacagcgt atgggccggg
     1621 cagaatgttt ctccgaaaga caaagtaaga attgagggtg aaattgataa agacctgagc
     1681 agtgttgaag ttgatgtaaa ggcactgaaa ttattaaagt aaccgcccct gcttgttaag
     1741 cccgtcttac tgacgggttt tctgtttgta cattccggcg tattgcctta caattcgcgc
     1801 agtcagcctg aacaactgac acctgctgtc accggagaat ccgatgacac aacacataaa
     1861 atcccacaat tctgaagccg acccggaaat taagcagggg aggcgttttc gtgcgcctca
     1921 gtatggctgg tttcactatc tgttctgtac gatcgatgag gcagatatgc ttcaagaggc
     1981 gtatctgcgt cgcggtgtcc gtgtggagcg gagtctgaac gctgatcgtc tgacctggac
     2041 cgtttctgta tatcttcctg ttcgtgcaca tctgccacgg acacatgcct gctaccgtca
     2101 gcgcgtctgg aggtaatgtg cgggtattac ttcgacctgt tctggttccg gaactcgggc
     2161 tggtggtcct taagcccggt cgtgaatcat tgccagtttt tcatcgcggc agggtgctgg
     2221 tggagccgga accgaaaaac atgcgggcgc tgccatctgg agcggttcct gctgttcgcc
     2281 agccgctggc ggaagataaa tcactgctgc catttttcag cgatgagcgg gtgattcgtg
     2341 cagctggcgg cgctggtgca ctgtctgact ggttattacg tcacgtgaaa tcctgccagt
     2401 ggccacacgg cgattatcat cacagcgaaa ccgttattca cagttacggt gctggcgcaa
     2461 tggtgttgtg ctggcactgc gacaaccagc tgcgcgacca gacctccgaa tcacttgagc
     2521 aacttactca acaaaatctg acagcctgga tgattgacgt catacgccat gtaatgaatg
     2581 gcacgcagga gcgggaatta tcgctggctg aattatcctg gtgggcagtc tgcaatcagg
     2641 tggtggacgc attacctgag gcagtatcgc gtcg
//
LOCUS       DAZUFB010000254         2630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZUFB010000254 DAZUFB010000000
VERSION     DAZUFB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.258"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            427..570
                     /locus_tag="ACHY3M_005204"
     CDS             427..570
                     /locus_tag="ACHY3M_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFS4319905.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            583..792
                     /gene="cspE"
                     /locus_tag="ACHY3M_005205"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             583..792
                     /gene="cspE"
                     /locus_tag="ACHY3M_005205"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS4319906.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            958..1767
                     /gene="rlmA"
                     /locus_tag="ACHY3M_005206"
     CDS             958..1767
                     /gene="rlmA"
                     /locus_tag="ACHY3M_005206"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFS4319907.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1764..2330)
                     /gene="mntP"
                     /locus_tag="ACHY3M_005207"
     CDS             complement(1764..2330)
                     /gene="mntP"
                     /locus_tag="ACHY3M_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFS4319908.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          699 a          614 c          625 g          692 t
ORIGIN      
        1 gattttttgc tcattcatca atttttctta ttttaaattt tcaatccctt tggggattga
       61 cttctcttta gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa
      121 ctttttattc attgcatagc aaaaaatgtg atattgcacg cactatgtaa taacttctcc
      181 cactggcctg gaacaactga acttattgaa ctatgttaga aaatacgcca gtttaagtat
      241 ctgcctgaac tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt
      301 gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa
      361 tggcttgcca taattaacgt tgtatgtgat aacagatttc gggttaaacg aggtacagtt
      421 ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta aacacgttga cgttgaatac
      481 cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg
      541 ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt
      601 caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc
      661 aaagatgtgt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa
      721 ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta
      781 acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa
      841 gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt
      901 tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt atcctgccgg agttgtcatg
      961 tcgttttcct gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt
     1021 ccccagcgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag
     1081 cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tacaagcacg ccgcgcattc
     1141 ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg
     1201 cttgatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac
     1261 gcatttgccg atgtgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg
     1321 ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt
     1381 ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca
     1441 gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg
     1501 cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat
     1561 gcagaacaac tggaaggttt tacattacag cagagtgatg agttgtgtta tccgatgcgt
     1621 cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag
     1681 ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat
     1741 attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg
     1801 atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata
     1861 aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat
     1921 gcggtcgcga taatgttgac ctgcaggaaa gcaagaccaa cacccacagc catggcatcc
     1981 aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct
     2041 tcatcttcat catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc
     2101 agcagcacaa acgcaatcca gtggttccat tcaaggacaa accggctggc taacatgccc
     2161 attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc
     2221 aatgcttcag aaaatttcgg tttatggagg gtggcacctt taccgattga tgcagcaaat
     2281 gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat gacaatgtcc
     2341 tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca
     2401 agccgcgatg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
     2461 gactgaaagt attcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
     2521 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
     2581 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg
//
LOCUS       DAZUFB010000255         2563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZUFB010000255 DAZUFB010000000
VERSION     DAZUFB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.259"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..252)
                     /gene="escO"
                     /locus_tag="ACHY3M_005208"
     CDS             complement(<1..252)
                     /gene="escO"
                     /locus_tag="ACHY3M_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFS4319909.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSISKEKRSLLDSQKRTKE"
     gene            complement(312..1652)
                     /gene="escN"
                     /locus_tag="ACHY3M_005209"
     CDS             complement(312..1652)
                     /gene="escN"
                     /locus_tag="ACHY3M_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFS4319910.1"
                     /translation="MISEHDSVLERYPRIQKVLNSTVPALSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYNN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQSFIQQSTKDISSYEKTIENLFKVVA"
     gene            complement(1636..>2563)
                     /gene="escV"
                     /locus_tag="ACHY3M_005210"
     CDS             complement(1636..>2563)
                     /gene="escV"
                     /locus_tag="ACHY3M_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFS4319911.1"
                     /translation="QQIEVMRWNFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYK
                     DTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNEANQQLAHLSGMDVYATTNDKI
                     TFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEE
                     NISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVRGTLLNVWLIGSD
                     IENELRESIRQTSSGSYLNISPERSEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMI
                     EGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          732 a          614 c          467 g          750 t
ORIGIN      
        1 ctcttttgtt cgtttttgtg aatctagcaa tgaacgtttc tccttcgaaa tggaggattt
       61 tttctcatac aatcgctgct tttgctcatc aaatgcattg tttttaatct gatatttata
      121 caacgacact ttatccaaat tcaccagcga tgcacaatac gcctgcttag aggcaatcga
      181 ctctttaatg gattgctcgc agtcatcgag tttaccatcc acctctttta tctgagagtt
      241 aatcttagcc attgattctc tcaatcggtt cgctctgctt ttacgaatag ataaaattct
      301 gtccaacata ctcaggcaac cactttgaat aggttttcaa tcgttttttc gtaactactg
      361 atatctttgg tgctttgctg tataaagctc tgaattaatt tcctattttt tattgcctta
      421 tccgcttcag gatcttgccc catcgtgtac tcaccaatac gaataagcag ctcaacattg
      481 ttataagtcg caataagctt tttgcattct gccgccgcac gcaaatgctc cgatgtaaca
      541 acgttatgca taacacgact ggcacttaaa ccaatatcaa ttgcagggaa atggttttcc
      601 tctgcaagtt ctcgggtaag tacgatatgc ccatcaagaa tagagcggac ttcatcgcca
      661 ataggatcat taacattatc gctttctaac aacaccgtat aaatcgcggt tattgaacct
      721 tttggcgcag gccccgctcg ctctaataat ttaggtaacg aggaaaaaac actcggaggg
      781 aatccccccc ttacatcagg ttctcccgat gcaagcccaa catcacgcgc ggctcgggca
      841 taacgagtta cagaatccat cattaataat acatttttac cctgatcgcg gaagtactct
      901 gcaatagtcg tcgccgtaaa tgcggctttc atcctttcca gtgcggggcg gtctgacgtt
      961 gtgacgacca gcacacattt agaaagcgtg gattgaggta aaagcgcgag gaattcattt
     1021 acttcgcgac cacgttcgcc gataagagca aggacaataa tatctgcgga tgcgccatta
     1081 caaatcatcc ccaaaagcgt acttttacca acgcctgaac cggcaaaaat accgatacgc
     1141 tgcccaatac cgcaagttag taacccatca attgccctga cgccaagtat aaatggctga
     1201 tcaataacct gccttaataa aggatctggt ggttcagcat acaagctgcg ttcaaacgga
     1261 agatagggag cagcaatatt actctccatt ggtctgccaa tgccatcaac aagccgccca
     1321 agtagtgcgt ctccaacacg aatcttgaac tcatccccct gataacttaa ccactgacca
     1381 cagtacatgc cggaaacatg ctcaaaaggc aacaggaaaa cctcatcttc atcaatagcg
     1441 atcacctcgg ctaaacgttg actgggttct atcttgtaaa aggcgccaat acgcgctttt
     1501 ggtaaacgcg ccttaataat cgtcccgccg atattggtaa ttttaccttc atatctggta
     1561 gacgaattta atgataatgc cggcacagtg ctattgagca ctttctgaat acgtgggtat
     1621 ctttccaata cagaatcatg ctctgaaatc atttaccgtt cctaatactt taagttcgat
     1681 attattccca acctcctgaa aagagagcac gggtactgac gggaacgaac cttcaatcat
     1741 tttcttcaca tagcgcctga tatctaaagc agtcagaatg acgccgttcc ccgttggatt
     1801 catgatattt tttaagaagc caattatctg ctcacttcgc tccggtgaga tattcagata
     1861 cgagcctgat gacgtttgtc tgatcgactc tcgtagctca ttttcaatat cagagccaat
     1921 aagccaaacg ttcagaagtg taccgcgaac gctatagcga cctaaaatat gccgacgcag
     1981 ggcgatacga acgtattcgc acaagataac cacatctttt tcttttgttg accaaaaaat
     2041 aagcgtttca aagatagttc tcagatccct aattgagata ttttcctcta cgagacgttg
     2101 gagaatgtca acaattttgc tcaaaccaag ctggcgctgc agctctttca caagctcgtt
     2161 atattttctc tccatgatgt ccatcaaata acgcgtttct tgtacgccga tgaactcttt
     2221 ggcattagac aatacaagtt tctttagcaa aaacgttatc ttatcatttg ttgttgcata
     2281 aacatccata cctgaaaggt gagctagttg ttggttagcc tcattcgttt tatatacgat
     2341 agagttagtc gaaagctttt cctgaacaaa ttcgaatgag atctctgcat gcccagcctc
     2401 aaaatagaca gtatcatcta taagagtatc tttgtatatt gactcttgat agagcaaaaa
     2461 ttctattgcg ctatcattat ttttaaccgg attgacaata atcttaggca atggaattcc
     2521 actttcctca aagaaattcc accgcatgac ctcaatttgc tgt
//
LOCUS       DAZUFB010000256         2479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZUFB010000256 DAZUFB010000000
VERSION     DAZUFB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.260"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..106)
                     /gene="gpU"
                     /locus_tag="ACHY3M_005211"
     CDS             complement(<1..106)
                     /gene="gpU"
                     /locus_tag="ACHY3M_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFS4319912.1"
                     /translation="MKHREIRAAVLSALKDNISERVSWFDGRPVFIDEQ"
     gene            complement(119..742)
                     /locus_tag="ACHY3M_005212"
     CDS             complement(119..742)
                     /locus_tag="ACHY3M_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319913.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(745..1026)
                     /locus_tag="ACHY3M_005213"
     CDS             complement(745..1026)
                     /locus_tag="ACHY3M_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS4319914.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1019..1345)
                     /locus_tag="ACHY3M_005214"
     CDS             complement(1019..1345)
                     /locus_tag="ACHY3M_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFS4319915.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1433..>2479)
                     /locus_tag="ACHY3M_005215"
     CDS             complement(1433..>2479)
                     /locus_tag="ACHY3M_005215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFS4319916.1"
                     /translation="NFVGDGIRQAMLARAGFENVEKDNAYNGMTLREWARMSLTERGI
                     GVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFK
                     TAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALATYGEIFSITRQAIINDDLNML
                     VDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQ
                     MMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVI
                     AEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGV
                     APLDWRGLVRSSVA"
BASE COUNT          512 a          796 c          610 g          561 t
ORIGIN      
        1 cctgttcatc aataaaaacc gggcgaccgt caaaccagct caccctctca gaaatattgt
       61 ctttcagggc agacagaact gccgcccgta tttcacggtg tttcatacac cctcccagtc
      121 attttctttt cagcaccagg cgtaactgat gcgtcatggc tttcatcatc tgcgccggta
      181 atttttcccg gtacatccgg tcccgttcac gttcaaaggt ttctgccagc ggtccggcag
      241 tcgtaatctt caccacttcg atcggcagac ggtggcgttt cggcctccct ttgctgtccg
      301 cgccggtgga tgatgatgcc cacggcatac gctgcatcac atgccagcgt ccgttagcca
      361 gccgggtgat aaaggcgtcc gggatccgtc ttttccccac aatcagcaca ctgccgcccc
      421 ctttcagggc cgcacgctgt cctttctttc tccgttttct gcgggaaagt cgaacgcggg
      481 cctcccccag tttgatggcg ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct
      541 tatccggtcg tgccggactt aaccggatgc gggcacggat aagacgacgg ggaacggcca
      601 gctccctggc aactgaagag gccgttttcg caataatggc ccccgccact cggttcagtg
      661 tcgtggcaga ggcccgggga acggcacggc gatcaattgc atccagattt ttcatggcct
      721 gcgccagacc ttttattgcc atactcattc ctgttcgaca aaaatccggg gtttaccgtt
      781 gtacgtgtca taacgggtca ccgtcagtgt gcgccccgca aacacaacaa catcatgacg
      841 ggccggacgg taccgggctg aaaacaccac cagtgacaac tggctgcccg aaagcgcccc
      901 catctccgcg gactcttcct ccggcatcac gtcgtacacg acgccgttaa tctccgcctg
      961 tttgcccatc acccgaacgg tcgccacgtc catccggcaa cacattcgcg taaacagatc
     1021 agacattgat ttttaccgcc acagtggcgc tgtttgcagg ggcattttcc caggctaccc
     1081 ccgctgccac cgcaccgtct gcagccagct gcacaacccc gtccttcaga taaaccaccg
     1141 cgccggactg aatgtcgtca gcagactgtt tgggcagcag gaacacgcct tcggcaaaac
     1201 cgtcaccggc ctcaccggca ggaatatcgg taatggccac ggccaccata ctgccgacca
     1261 ccaccgcagc accgctcagg atggtctgat ctccggcatt caccagttca atggtggtac
     1321 cgtcctgtac aaaatttttc gccataatgc tgtttctccg gacagcccct gtggggctgt
     1381 ttttcaggca taaaaaaagc cctttcgggc agtgattgtg ataacgcggt tatcaggcca
     1441 ccgacgaacg caccagcccg cgccagtcaa gtggtgccac tccggcatca atacggattt
     1501 ttgtggcaat gccgtcagtg gtgaaacctt cctgctgatc aatgtatggc gtgtccacac
     1561 catccagcca ggccacttca atggtgtcag tgccctgtgc cgccgccaga taccaggttt
     1621 tcgggtctgc cgcatcaaga cgcgcttctg caatcacctc agcaaagttc tggatagggt
     1681 taatgacacc ggcgtttgca tccgcccctt tcacactggc cgatttgatg gtctggttcg
     1741 ccaccgtctc cagtgccacc ggtaccagca taaaggccgg acggatattc agggcacgat
     1801 cgccttcttt ctgcaggcgc atcatctgac gggccgcatc cagtccggaa acggagatcc
     1861 ccccggtggc aatatttttg tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg
     1921 ggttatccgt cagcaccttg tagaccaggt caccaatcgt tgccttcgcc gcacgcccca
     1981 tcttcatcgg cacgtccacc agcatattca gatcatcatt gataatggcc tggcgggtga
     2041 tggagaaaat ctccccgtaa gtggccagtg caatggtctc cttgcgatct gaggtggtga
     2101 tgtatttata ctccgccccc tcacgaacct ggcgcagaga accaaaaccg cccatcccca
     2161 cgcgatacgc tgttttgaag tctgacaggc gtcccttacg ggtccacttc tggaaggttt
     2221 cttctgattc ctcccagccc tggatcagcc ccttgttcga cacatccagc agaatattgc
     2281 caaaatcaga ggtgctgtgc gtcagcgcca gcccgaccat ctgcatgggg ttataactgg
     2341 ccaccccaat accgcgctcc gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt
     2401 tataggcgtt atccttctcg acattttcaa atccggcacg ggccagcatc gcctggcgga
     2461 tcccgtcccc cacaaaatt
//
LOCUS       DAZUFB010000257         2471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZUFB010000257 DAZUFB010000000
VERSION     DAZUFB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.261"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..185)
                     /locus_tag="ACHY3M_005216"
     CDS             complement(<1..185)
                     /locus_tag="ACHY3M_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4319917.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRL"
     gene            complement(267..1088)
                     /locus_tag="ACHY3M_005217"
     CDS             complement(267..1088)
                     /locus_tag="ACHY3M_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS4319918.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPSITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(1309..1719)
                     /locus_tag="ACHY3M_005218"
     CDS             complement(1309..1719)
                     /locus_tag="ACHY3M_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319919.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            complement(1735..2418)
                     /locus_tag="ACHY3M_005219"
     CDS             complement(1735..2418)
                     /locus_tag="ACHY3M_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319920.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
BASE COUNT          601 a          605 c          604 g          661 t
ORIGIN      
        1 agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg aatgaggcca
       61 aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgaggctggc aaccaactga
      121 ggcggattat tctctggtgt tgtagctgaa taaattgtgg gggtattctg agaaacggtt
      181 ttcatgagat aactccttaa ataacaaata aatgtgtgtt tttcaggaac gaaagctgaa
      241 tgattccctc actcagggaa acggcatcag cgcaggctct ccagcatcgt ttctgccatc
      301 acccacaatg cgcgattgag cttaatgtcg gtatcaatgc tgtgaatggc ccgggtatgg
      361 atacgttttc cttttgcact gcgaccggaa atcccgcctt tcagcatatt ctcctggatg
      421 gtctgatatg cgctccacag gtccttaccg taatcctccc ggcgtcgcgg cgtcagaatg
      481 tcggcggtgg tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      541 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttctcctct
      601 atccggtcaa acacgcccac cacctcgtaa gccccttcga taactttctc cactacattt
      661 ccccggtgtg gaacacgcac ttcccccaga gactggccac agacgcaccc gttctggcag
      721 acgaacctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      781 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      841 tgttttgtgt atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaacggc
      901 tgaaagcctt cccgctgcag attttccagg acggtaatgg agggaatgta cgcataccgt
      961 tcactgcggg aggtgtgccg gtcttcccca aaaatactgg gtacatggcg tatcagttct
     1021 tcatgtgtca gcggacggtc acggcgtatc tggtttgcag cataaccaaa acgactggct
     1081 aatcgcatac tttgctcctt atcggtggtt aagatttact ggtgcaataa atgaaaaaag
     1141 ccgtgtctcc cggagaagac gcggcctgac gggtgaaatg aataaggttt attgtctgag
     1201 aggcctttca ctggcgagcc acgcattaag cggctttccg gtaacaatgg ctggggctgt
     1261 atttaccttt gatattaata atgtgcagag aaacgcatgg tttactcctt agattttaag
     1321 tttgtcctgg gctacaccga caggttttcc ggatgtgtcg atgactccta ccgtcggtac
     1381 gccatttttg atttcgaaga acggtttgtt gctacctgag cacagttgat tgtccccgta
     1441 ttcaatcatt tgtgctttcc cggttttcag gttaactacc gagacagtgt agtcgtagca
     1501 ccaggcacca ccacgttcaa gcacgaaaac atagctgtta tctttatagt tgaactgatt
     1561 ctttgtctgg caattcatac cgtcaatttc ggtacaggaa aacgcttctg tagtattaac
     1621 gtatttgatg ttatttggca gttttacttg tttcgcagta gcgaagacag agaacagaat
     1681 agttacagca acgagtaaag ttttgataaa agttttcatg atagactcct taaattagtt
     1741 aaccgcagct acgtagtttt tcaccagata cgcgaattca tgcacgccaa tttcaccgaa
     1801 ccaaaccagg taggccgggt catctaaacc aaagtaacgg tagccaacaa cttcatgggt
     1861 ttttgggtta tagagtgtta cgataaagtc ccagccgcag gtattggctt tgcaaccgtc
     1921 gccctggaac cagactgtac cgttaatgtt tactggcgtg atacctccac cagggccatc
     1981 catatagccg cctttaccga acatgccgct cagtccgacc ggttttaatg ctttgtcgat
     2041 agcggctttg taggtagcgt caccttcgta caactcgtac gttgatggac acgctccagg
     2101 ttgttcagcc caggcttccg ggttacaact aagagcttgt tttacggtag ccgcttctgc
     2161 gttaaagtta aagcccgtga acagaaccgc cagaaataac ataagggtat gtaaacgttt
     2221 cattgtaaat attcttccgt tgtaaatggc gctggaaaat ttctcatcag tgaaaccttc
     2281 cggaagtttc ccccccttgt atgatgtata tcgccagtat tttatttcat cccggctgta
     2341 atttttatat tccccggcac gtgcaataac gcggaccaga gatataaggc tcattaataa
     2401 ctttacaggt aatttcataa ttaaatttcc agtgagtgaa ttgacaggtg aggggtaaaa
     2461 ctttctctca g
//
LOCUS       DAZUFB010000258         2471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZUFB010000258 DAZUFB010000000
VERSION     DAZUFB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.262"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..570
                     /locus_tag="ACHY3M_005220"
     CDS             <1..570
                     /locus_tag="ACHY3M_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HFS4319921.1"
                     /translation="LDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAEN
                     TQVEAVKGGRSGAMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYW
                     LPPSLPGWAIPASDNKPSPENTNQKSPSVDCEQILKDFSDYAATETDKKKLIERYQRD
                     WQLMAGNEEAQAKCVQVMNIRVNELKQEA"
     gene            571..1083
                     /gene="ssb"
                     /locus_tag="ACHY3M_005221"
     CDS             571..1083
                     /gene="ssb"
                     /locus_tag="ACHY3M_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4319922.1"
                     /translation="MGRGVNRVIIIGRLGQDPEVRYSPSGTAFANLTVATSEQWRDKK
                     TGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEVI
                     VGVGGAMQMLGGKQGSNEQSSPQRNNGQQQRQQSQQHGNYGEPPMNFDDEIPFAPVTL
                     PFPRHAIHAI"
     gene            1097..1390
                     /locus_tag="ACHY3M_005222"
     CDS             1097..1390
                     /locus_tag="ACHY3M_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFS4319923.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYARIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            1401..1568
                     /locus_tag="ACHY3M_005223"
     CDS             1401..1568
                     /locus_tag="ACHY3M_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFS4319924.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKTMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGKQ"
     gene            1565..1999
                     /locus_tag="ACHY3M_005224"
                     /pseudo
     CDS             1565..1999
                     /locus_tag="ACHY3M_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            2166..2344
                     /locus_tag="ACHY3M_005225"
                     /pseudo
     CDS             2166..2344
                     /locus_tag="ACHY3M_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          759 a          534 c          637 g          541 t
ORIGIN      
        1 ctggacgatg tttgcggcaa agcaggatgg cgcaatgaat accgcgatat tcccaacaac
       61 ggcggcgttg aatgcggaat atcaataaag attgattccg aatgggtaac caaatgggat
      121 gctgctgaaa acacgcaggt agaagccgtc aaaggtggtc gttcaggtgc aatgaagcgt
      181 gctgccgttc agtggggaat cggtcggtat ctgtataacc ttgaggaagg tttcgcacaa
      241 acatctctcg ataaaaagca ggggtggcac agggcaaaac tgaaggatgg aacaggattt
      301 tactggctcc ctccatcact tccgggatgg gcaatcccag catcagataa caaaccatca
      361 ccagaaaata ccaaccagaa atctccatcg gttgactgcg aacaaatcct gaaagacttc
      421 agcgattatg cagcgacaga aactgacaag aaaaaactca tcgagcgtta tcagcgtgac
      481 tggcaattaa tggctggcaa cgaggaggcg caggctaaat gcgttcaggt gatgaacatc
      541 agagttaacg aactaaaaca ggaggcataa atgggacgtg gagttaatcg cgtaatcatt
      601 atcggtagat taggccaaga tcctgaggtg cgctattcac catcaggaac ggcatttgca
      661 aaccttacag ttgctacgtc agagcaatgg cgtgataaga aaactggaga gcaaaaggag
      721 cagacggagt ggcaccgcgt ggtaatgagc ggaaaactgg cagaaattgc cagcgaatat
      781 ctgcgaaaag gctctgaggt ttatcttgaa ggtaaattgc ggacaagaaa atggcaggat
      841 caaagcggac aggataggtt cactacagaa gttatcgtgg gcgttggtgg agccatgcaa
      901 atgcttggtg gcaagcaagg aagcaatgaa cagtcttcac ctcagcgaaa taacggccag
      961 caacaaagac agcaatctca gcagcatggg aattacggcg aaccacctat gaacttcgac
     1021 gatgaaatcc cctttgcacc agtaactctc cccttccctc gtcacgctat tcacgcaatt
     1081 taatcaggag aaaatcatgc cagcgcctca gtatggtgcg gatgacccgc gccgctgttc
     1141 cggcaattcc gtatcggagg tgctggataa attcagaaaa aactacgccc ggataatgtc
     1201 gctaccgcag gaaacgaaag aggaaaagga atttcgccac tgtatatggc ttgcagagaa
     1261 agaagagcgc gagcgaattt accagacatc aatccgacca ttccgcaaag ccacatatac
     1321 ccacttccct gaaattgacc cgcgcctgcg taattaccgc tcacgctatg gcgctatcag
     1381 taatgactga ggaatttacc atgagaggac ttgcatacaa tcccggcatt cttccggcag
     1441 aaatgattat tcgccaacgc gtaaagacaa tgccatcgag agaggaattg cttaagagaa
     1501 attcttttcc gtcagtgaat caaaacaaat atctgaatgc gatgtggcgt aaaggaggca
     1561 agcagtgagc gaaattaatt atcaggcact gcgtgaaaag gcagagaaag caactaaagg
     1621 aagctacatc gtagggcata catctgttaa ccagcacggc aatttaacag gagtttttgt
     1681 ttgccaaaaa tggaaaggag aacccggtgg tgtgattgcg gaatgtcatg ttaactgcct
     1741 ggttgaaaca gatgttcagg cttatgcaaa cgctgaattt attgctgcct ttaatccaaa
     1801 tgttgcgctg gcgcttctgg atgaacggga aagaaaccag caatacatca aacgccgcga
     1861 ccaggagaac gaggatattg cgcttacggt tgggaagctg cgcgttgagc ttgaagcagc
     1921 aaaatcaaaa ctcaacgagc agcgcgagta ttacgaggga gttatctctg atgggtgcaa
     1981 gcgtattgct gaactggaag cgcgggaagt tcaattaccg actcgctacg accttcgata
     2041 tggacacccg ataaatgcag atgagcgaca agtcatgata cctaaagaaa atggcagttg
     2101 gctttacctg attgacctag aacacgcatt acgcgtcgct gacattcgca tcaaaggtga
     2161 gtgatatggc gttaacacac cgcgaactct gtcagattgc gtacaagttc cttaagcgca
     2221 acggtttcaa ggtttgtttt catgaccgct ttatagctgt aaccagtacc ggagaacagc
     2281 cagatgctat gggattcaga aattcagcat catgcctgat agaggcgaaa tgttctcgtg
     2341 ctgacttgtt ggcagataga aaaaagcgtt ttcgtaaaaa tccgtctctt ggaatgggcg
     2401 actggcgatt ctttattagt gagccgggaa ttatttcaat tgaggattta ccacctggct
     2461 ggggattact t
//
LOCUS       DAZUFB010000259         2452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZUFB010000259 DAZUFB010000000
VERSION     DAZUFB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.263"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(226..600)
                     /locus_tag="ACHY3M_005226"
     CDS             complement(226..600)
                     /locus_tag="ACHY3M_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319925.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(612..764)
                     /locus_tag="ACHY3M_005227"
     CDS             complement(612..764)
                     /locus_tag="ACHY3M_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS4319926.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            complement(1037..1753)
                     /locus_tag="ACHY3M_005228"
     CDS             complement(1037..1753)
                     /locus_tag="ACHY3M_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4319927.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKADDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTEIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1803..2018
                     /locus_tag="ACHY3M_005229"
     CDS             1803..2018
                     /locus_tag="ACHY3M_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4319928.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            2015..2440
                     /locus_tag="ACHY3M_005230"
     CDS             2015..2440
                     /locus_tag="ACHY3M_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4319929.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
BASE COUNT          646 a          541 c          565 g          700 t
ORIGIN      
        1 aggaataacc ccgccatact taccgccgcg ccatttcgcg gattgccaca accggaagcg
       61 cacgatcgaa ttaaatttaa cgacgaccta tacagagaga ctaacttcgc cgggcgcttt
      121 cgtgttatgc cctgactttt cagggatata tccttttcag taaactgtca gtgccggatt
      181 cttatccgtg tccggcgcac gcactctacc tcacctgtga ataaattaat gattaattga
      241 tattttgttg tttgattcaa ctttcccatc ggatgtgtga tgctttaaat cacaggaatt
      301 aatactgctc gctgtaaaat gattttcaag gggagctatt cgaatccctt tctttttcat
      361 taacaagcca aatcctttat taatgatgtc cattaattcc aggaagtatt tttcatgtaa
      421 atcctggtta tcagagagct gcttctcttc gtacagaccg ataaaggcac gacgcacgtt
      481 accggatata gtatcgatgg tttctttttc tacggtactc aggtcaagag tcgccagttg
      541 agagcgaacc acattcgatg ccatttcctg gaatggtact ggtaaatctt taaattccat
      601 cgtcaacctc atcagtcagt gtttctggtt aaccagcgac gcgcgccagc ttcagtttta
      661 aacgttttgc ttctggtata cgtcatcgcg gtaaacgtgc cgtcctggtt gggaaacacg
      721 ccgtacacca gagattcgtt gttgccaaga tcgatagtat ccatgctgac ctcatttccc
      781 cttaacgctg gggtagcgga actaaaaacc tgctgcgctg ttatacaaag tgttcccgcc
      841 gtcatgttca tacgcctcgg gctggctact taccccctga ccactgcttg gtaactcgaa
      901 gtattgcccg gcgttctgtg gggcggggtg ggttggtatg ttgttaaggt aacaagagtt
      961 accttttggg ttaatacaat gttgcaaaag gtacatttga gggcatgaaa aacccgcaat
     1021 gaatgcgggt tctgactcag tctaagtatt gatgtatttg tgaaacttta cctttaatgg
     1081 tgtaaccacc attcagttcg atgggtttgt aaagcggatt cagtgacaac agatagatgt
     1141 ttggtccgtc aatcgcaact ttttttagtg ttacgtttgg cgttccttcc aattggatta
     1201 agattatttt tcccaccagt tctctaatgt tacttgagca tggtgtgatc agcacggtag
     1261 atccgtcggg gatggttggg aggccgttag agtttgtcat cgcatctccc tcaacatgca
     1321 ataaaaaaga gttttcagcg gtttttgtca tgacatcaac ccaattctta ataccaggaa
     1381 tctcggttac tggacaactc atatcccaat aaccagcctg ttcccacgtt aaaacgggca
     1441 accgggcgat gttgtcacta atgtaagggt actgattcag acgcagatca tcggctttat
     1501 cgtgaccgtc ctttccataa agaatccatt caggagattt ggaaagcaat tttgacagta
     1561 gatacaaatt ctcaccgtca ggttttgaag agccattttc ccattttgtt acggatacac
     1621 gagatatgcc gattgctttc gcaacctgct gttgggttaa tccaacgtct tttcgacgat
     1681 tccgaatacg ttcgctgata gtgtttttca tgtaaccaat gttactacca agtgatgttg
     1741 ctatggttga cattgttatg taactattgt tacccttcta ctcgaaataa caggagagtt
     1801 ttatgttcaa agatgatgtt ctgcgctatt tcaaaaaaaa gcgactagta gctgaggctc
     1861 ttggaatttc acatgtggct gttgtgcggt ggaaagcagt tattcccaaa cttcgcgcaa
     1921 tggaactgga tgaaattact aacggtgaat tgaaatacaa cccagaactt tacaagaagc
     1981 aggatagcac ctcaaacgaa ggaaagaatg attcatgaaa atcaagcatg aacacatccg
     2041 catggcgatg aatgtctggg cgcatccgga cggcgaaaaa gtaccggctg cgaaaattac
     2101 caaagcgtat ttcgagctgg gaatgacgtt cccggaactg tatgacgaca gccatccgga
     2161 agccctagcc cgtaataccc agaaaatttt ccgctgggta gagaaagaca cccctgatgc
     2221 agttgaaaaa attcaggcgt tgttaccagc gatcgaaaag gcaatgccac ctttgctggt
     2281 ggccagaatg cgcagccaca gttcagctta ttttcgggag ctggtggaga cgcgggagcg
     2341 actggtgaga gacgctgatg attttgtagc agtggcaatc gccggtttca atcagatgaa
     2401 ccgtggtggc ccggcaggaa atgctgtggc agttcattga ctgacaatag cc
//
LOCUS       DAZUFB010000260         2434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZUFB010000260 DAZUFB010000000
VERSION     DAZUFB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.264"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..339)
                     /gene="espW"
                     /locus_tag="ACHY3M_005231"
     CDS             complement(<1..339)
                     /gene="espW"
                     /locus_tag="ACHY3M_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFS4319930.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVR"
     gene            complement(418..1068)
                     /locus_tag="ACHY3M_005232"
     CDS             complement(418..1068)
                     /locus_tag="ACHY3M_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFS4319931.1"
                     /translation="MPGILNFSNGSVLPENELEALRHIARNNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            1251..1841
                     /gene="espM2"
                     /locus_tag="ACHY3M_005233"
     CDS             1251..1841
                     /gene="espM2"
                     /locus_tag="ACHY3M_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFS4319932.1"
                     /translation="MPMNTTGMSFSSFGISCHKENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYYFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            2075..2227
                     /locus_tag="ACHY3M_005234"
                     /pseudo
     CDS             2075..2227
                     /locus_tag="ACHY3M_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2343..>2434)
                     /locus_tag="ACHY3M_005235"
     CDS             complement(2343..>2434)
                     /locus_tag="ACHY3M_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4319933.1"
                     /translation="RTADKDSAKTFVQETLKDTWKSADEWFVH"
BASE COUNT          739 a          441 c          520 g          734 t
ORIGIN      
        1 tcgcacatta tttaactttt ttaaaaacaa agagtcaatg tctattcggg agcgtttatc
       61 atcactgttt atggcatagt attgggctcc gctcctgctt cccattcctg gggtccctgc
      121 aataataaat gagtgacttc ttgtatgtct gccattgagt agagcaaggg gagttttagc
      181 tgtagccaac catacaggaa cggattttaa agatatacca tattctttat gaacaattct
      241 tctggagacg gggttcgtca ttgttgtttc atttgaaagt tgttgatgtt cactgaacag
      301 atggtaacat gatgatacaa ctgatgatat tttgggcata aatagtctcc tcgctatatg
      361 tttgttatat ggtgattaac ttattgaaaa tatacttctt atattgtgct tttattttta
      421 aatactgttt tgttgaagtg ggtatatggg taatgcgtaa aaacattgtt ttgttatatt
      481 aaaatgacac ttgtctttgc ttataatcat ttctggagca aaggggtcac ggctgagggg
      541 gtggagagcg ttacgcagga taagttcagt caatgcttcc tgatcatata atgcacatat
      601 ttctgaattt cgagcatttc tgacaaaaac acctgtctca ggaatatgta gcgttatcgg
      661 acaattaaga tgttcctcag tacaggaaaa gtcttgtaaa ctaacgggaa atgcattcga
      721 atttattcga tctagtagtg tttttatgac gctttcctgc acaagcgggg cagcttgcct
      781 ctgctccaga tataagctgt atgcctgcag aaaagtgtta cctccgttaa gttgtcttgt
      841 caggcggtct gcgagatgat gagcctctct tgccccaaga tggtcccaaa gtccaccaag
      901 aataggctca acactaaaac catccataaa ctggatataa tgaagcctca tattacggcc
      961 tcctatagta atagtgtcat tctggttgtt tcttgctata tgtcgtaaag cctccagctc
     1021 attttccggc aatacacttc cattagaaaa atttaatatg cctggcatat tattatcctt
     1081 tttaatccat attgtgaact atattggtaa caagaatcaa cataccgctc atgatatgtg
     1141 aataaaacaa tacaattttt aatagaatat tattgcatgc atgttttttc tggctacaat
     1201 aaacgcaagt atgcaatatt gtaaacactt ttttataaaa aggcattata atgccgatga
     1261 atactacagg tatgtctttc agctcttttg gtataagttg ccataaagag aactccttca
     1321 ggaactcttt ccgagggaag aatgatgagg tcgtaaaatg ttcaattgga gaacggacta
     1381 ttagcttttc tgttcgcaaa tttagcggca atatactgga aacggtgaga aggcagagta
     1441 ctaaagatat tgatgagtgg ataaaagatg aacggatagt atatccctca agggtgatca
     1501 accaggagat tgataattac tattttcaaa aaaatgcaaa aatttccact gaagagaggc
     1561 aaagagtctt ttttcttgtg agccaggaga atcaactaac ccttgatgtt aaggcagcac
     1621 aaagttctat taatcatgta ataatgggga gtgcttcttt tggcaagaaa atggatgctc
     1681 tttgtgatgg tatgagccgg gatgtaaaaa atcgtacatc agataccata gcaaacttgc
     1741 ttgcggacaa gttttaccag aaacatatcg attcggatat tgatattgta aaactacgaa
     1801 atgatatccc agattatttg atgcgtgcta tacagggatg aggctagagg agaggactgc
     1861 ttcagcatca tcggcttcgg accagtaaaa gagacgcaga aaatgcacaa cggcaccaca
     1921 cgccatgcat ggattcagat tactcatggc tcaccagtac agctataaat cgtaaagaaa
     1981 actacagtac gttatacaca gaaacgtacc gtgattcatt atttattgta caggttcatc
     2041 gcagcgacaa tattttgtga tgtttgcgtg gtgcgcccca cttcaggatg ctgcagatct
     2101 ggaaattgca acggaggaag aaacctcatt gctggaagcc tagaaaaagt atcgggtgtt
     2161 gctgaaccgt gttgatacat caactgcacc tgatattgag tggccggaag aaccagacac
     2221 aatgtaagcg aaaaagaaaa accgcagaca cgacgtatgc aggacgtgct gcggttggct
     2281 ggtgaacttt cgatagtgcg agtattgaat gatttccagc cgttaccgat tttacgtgtt
     2341 aattagtgaa caaaccactc gtcagcagac ttccaggtat ctttcagagt ctcctgaaca
     2401 aaagttttag ctgaatcttt atcggcggtg cgcg
//
LOCUS       DAZUFB010000261         2412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZUFB010000261 DAZUFB010000000
VERSION     DAZUFB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.265"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1193
                     /gene="escC"
                     /locus_tag="ACHY3M_005236"
     CDS             <1..1193
                     /gene="escC"
                     /locus_tag="ACHY3M_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFS4319934.1"
                     /translation="DIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            1207..1662
                     /gene="cesD"
                     /locus_tag="ACHY3M_005237"
     CDS             1207..1662
                     /gene="cesD"
                     /locus_tag="ACHY3M_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFS4319935.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2046..>2412)
                     /gene="grlA"
                     /locus_tag="ACHY3M_005238"
     CDS             complement(2046..>2412)
                     /gene="grlA"
                     /locus_tag="ACHY3M_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFS4319936.1"
                     /translation="KNYDGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRASFIIT
                     YISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGSTGPKRKTYRVGNG
                     IVGQSNIWNEMILRRKKES"
BASE COUNT          796 a          465 c          436 g          715 t
ORIGIN      
        1 tggatattga ctcactgcta aaatatctga gtgatacaat ctcagtaaac aaaaactctt
       61 gcaatgtgag aaaaattacg acatttaact cgattgaagt tcggggtgtt cctgaatgta
      121 taaaatatat tacatcttta tcagaaagtc ttgataaaga ggcacagtct aaagccaaaa
      181 ataaagatgt tgttaaggtc tttaaactga actatgcgtc agcgacagat ataacataca
      241 aataccgtga ccaaaatgtt gtcgtcccag gtgttgtctc cattctgaag acaatggcaa
      301 gtaatggttc gcttcccagt accggtaaag gtgccgtcga gcgaagcggt aacctgtttg
      361 ataacagtgt aacgatttcc gctgatccac gtcttaatgc agttgttgta aaagaccgcg
      421 aaataacgat ggatatctat cagcaattaa tttctgagct tgatattgaa caaaggcaaa
      481 ttgaaatttc tgtctcaatt attgatgttg atgcaaacga tcttcagcag ctcggcgtaa
      541 actggtccgg tacgttaaat gcaggacaag gtacgattgc ctttaatagc tctaccgcac
      601 aggctaatat cagctcttct gtgataagta atgccagtaa ttttatgatt agggtgaatg
      661 cgttacaaca gaactcgaaa gctaaaatac tctctcagcc gtcaattata accttaaata
      721 atatgcaggc gattcttgat aaaaacgtaa cgttttatac aaaagtctcc ggagaaaagg
      781 tggcgtctct ggaaagtatt acaagcggaa cattgctgag agtgacacca cgtatacttg
      841 atgacagctc gaatagtctt accggaaaac gtcgggaacg agttcgtttg ttacttgata
      901 ttcaggatgg caatcaatcg actaaccaaa gcaatgctca ggatgccagc agcaccttac
      961 ctgaagtaca aaattcagaa atgacaacag aagcgacatt aagtgcagga gaaagtttat
     1021 tacttggcgg atttatccaa gataaagaaa gttcaagtaa agatggtata ccgctgttaa
     1081 gcgacattcc tgttattggt agtttattct ccagtacggt aaaacaaaaa catagtgttg
     1141 ttcgactctt tttaattaaa gcaaccccga taaaatctgc atctagcgaa taataaatga
     1201 ggctttatga gcaggaaatt tagctctcta gaggatattt atgatttcta ccaggatggt
     1261 ggcacattag cgtcattaac aaatctgaca caacaagatc tcaatgacct tcattcttat
     1321 gcctatacag catatcaatc tggtgatgtt ataaccgcaa gaaatctatt ccatttgctc
     1381 acatatctgg aacactggaa ttatgactac accttatctc tgggcttatg ccatcagcgt
     1441 ttatcaaatc atgaagatgc acaactgtgt ttcgcacgct gtgcaacttt agttatgcaa
     1501 gatcccaggg catcttatta ttctggaatt agctacttac tcgtcggaaa taagaaaatg
     1561 gccaagaaag cctttaaggc ttgtttaatg tggtgtaatg aaaaagaaaa atacactaca
     1621 tataaagaaa atattaaaaa attgttaggt aatacagagt aaaaactaac atcagctata
     1681 ccaatcccca ggctgactgg ggattttcta tcggaaataa taaagctgct atagtgaagt
     1741 gctcccaaag ttgagcaccg accactggta gatttatcta caggtgtggg gttattccat
     1801 taactaggat tttatgccct gaactatctt ctgctgtatt taatatcatt tccgtttatg
     1861 aaacaatcag tatatcttaa cttagcatta gaaatctgat ctttccaatc atctatagct
     1921 cagagtatat ttgtcaggaa atgaccttca aactttagcc gagaaaaagg cttaccctgg
     1981 aaaacaaaac ccttaaatat agctttattt ttattcttct ataaaatata ctcaaaaaat
     2041 tacgtctaac tctccttttt cctcctcaag atcatttcgt tccagatatt agactgtccc
     2101 acaataccat taccaactcg gtaggttttt ctttttggtc cggttgatcc aggactttct
     2161 atcggaaccg cctcaaggtt aacatcataa atgaaaatct caaggcgctt ataatgcaaa
     2221 ttaccataac taacatatct gacacgaaat gaaatttttt cttttcttct cgatatataa
     2281 gttataataa atgatgctct cctcaggttt ataccgaatg cttcggcaat atcattgcga
     2341 gaaatccatt tctcctgcaa tttacaccaa agagaaacca agatatacag gggttcacca
     2401 tcgtaattct tt
//
LOCUS       DAZUFB010000262         2372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZUFB010000262 DAZUFB010000000
VERSION     DAZUFB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.266"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..557)
                     /locus_tag="ACHY3M_005239"
     CDS             complement(<1..557)
                     /locus_tag="ACHY3M_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319937.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
     gene            complement(577..837)
                     /locus_tag="ACHY3M_005240"
     CDS             complement(577..837)
                     /locus_tag="ACHY3M_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319938.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTF"
     gene            complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHY3M_005241"
     CDS             complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHY3M_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4319939.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1406..1510)
                     /locus_tag="ACHY3M_005242"
     CDS             complement(1406..1510)
                     /locus_tag="ACHY3M_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319940.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            complement(1626..>2372)
                     /locus_tag="ACHY3M_005243"
     CDS             complement(1626..>2372)
                     /locus_tag="ACHY3M_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4319941.1"
                     /translation="VAKIIAHYPLGVDVGKQKFVQAIRELPDFGGYLFAAPPAPIVPE
                     EMYWQDAPVEGSSKAAAYATGWNDCREAMFQSGNFRENKDSSTNNFRKIPEASTSSPV
                     TPALLPGGFTIEEAKELHEDLARSHISKALSGEKMKKKDRDADLRWIHGVIVQAAWFV
                     KASLEQNALSGNYPVTPDSWISCSERMPDTKTAVLVARDFGRKGDWRMKWATYIPGHP
                     DANDGWIIPGASWIPSHWMPLPEPPQEVRQ"
BASE COUNT          559 a          653 c          574 g          586 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcatggatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
      541 cgaagtaata cccgcacatt acctccagat ccgttgctag aatgtgcggg acggacgcgg
      601 tggccgttcg gagtaaggga gcctgacgga gattatccag tgacggtagt cgaggctaag
      661 ggctttttta acctcgcatc cgcgcctgcg gtaacactga atgagccatt cggcctgttc
      721 ttcagtgcat gggggatgct ggtaccagtc agatttgaat gcgtgaaaac accgtccgcg
      781 cctgctggca aagacggcag aatcatcaga attgtataat ttggtatcgt gcgccatcgg
      841 ttgtctctgc tggcgcagca ggtgccagtt gttcaggctg gcgtgcgaat tgtaaaccag
      901 aatgccagga aaaaacaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
      961 tgattcatca gaggtggcga gggatttctc ccccgccggg tctcttactc ctcaggttcg
     1021 taagctgtga agacagcgac ctccgtctgg ccggttcgga ctcgtacctc gcagaggtct
     1081 ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgatcag ggcgattaac
     1141 atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac
     1201 ctacgtttgt gaagcataga ttgggcctca gattaatgtt aagcgtcttg caggacgcgt
     1261 aatgttaact ggggcttttc tctgtctgcc ttacggtggc atgcccgagg cagacagcct
     1321 caagcacccg cagcaattct acttaactct cgctttaccg caaaccgttt ttacccgata
     1381 tgggaattcc catatcgtaa tgaattcagt tccctagtcg atccatcaaa aacacaacca
     1441 ggcagtaaac gcccacaaca gcaacaacag ccagcgcacc ttccattgcc agtgatatat
     1501 catccgacat attccctcct ttggtgttaa tcccggcgaa cgtttttacc ctcaccgaca
     1561 aataacatat actaaaaaag cgatagccat agcaacgcct gtaattgcaa atgcttcagg
     1621 ccagttcatt ggcgcacctc ctgcggcggt tctggtagag gcatccagtg tgatggtatc
     1681 cacgacgcac caggtattat ccacccatca ttagcgtcag gatgccccgg gatgtaagtc
     1741 gcccatttca ttcgccagtc acctttcctg ccaaaatccc tggcaacaag aacggctgtt
     1801 ttggtatccg gcattcgctc actacagctt atccaactat ccggagttac cggatagttg
     1861 cccgatagtg cattctgctc cagtgatgct tttacaaacc acgctgcctg aactataacg
     1921 ccatgaatcc agcgcaaatc agcatcgcga tctttctttt tcatcttttc gccacttaag
     1981 gccttgctta tgtggctgcg tgccaggtct tcatgtaatt ccttcgcctc ctcaatggtg
     2041 aaaccaccag gcagaagagc cggagttacc ggagagctgg ttgacgcttc cgggattttc
     2101 cgaaaattat tggttgacga atctttattt tcccgaaagt ttccggactg aaacatggct
     2161 tcgcggcaat cgttccagcc tgtagcgtat gcagccgctt tgctgctgcc ttcaactggc
     2221 gcatcctgcc aatacatttc ttccggcact atcggcgctg gaggggcggc aaatagatat
     2281 ccgccaaagt caggaagctc tctaatggcc tgtacaaatt tttgtttgcc tacgtcaacc
     2341 cctaatgggt aatgagctat aatctttgcc ac
//
LOCUS       DAZUFB010000263         2341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZUFB010000263 DAZUFB010000000
VERSION     DAZUFB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.267"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..887)
                     /gene="nleH1-2"
                     /locus_tag="ACHY3M_005244"
     CDS             complement(<1..887)
                     /gene="nleH1-2"
                     /locus_tag="ACHY3M_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFS4319942.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVLYDRMRNEFNPIDISSYNVSD
                     ISWSEHQVMQSYHGGKLDL"
     gene            complement(994..1065)
                     /locus_tag="ACHY3M_005245"
                     /pseudo
     CDS             complement(994..1065)
                     /locus_tag="ACHY3M_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            1059..1735
                     /locus_tag="ACHY3M_005246"
                     /pseudo
     CDS             1059..1735
                     /locus_tag="ACHY3M_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1739..2118)
                     /locus_tag="ACHY3M_005247"
                     /pseudo
     CDS             complement(1739..2118)
                     /locus_tag="ACHY3M_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
     gene            complement(2231..>2341)
                     /locus_tag="ACHY3M_005248"
     CDS             complement(2231..>2341)
                     /locus_tag="ACHY3M_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4319943.1"
                     /translation="TQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          698 a          567 c          445 g          631 t
ORIGIN      
        1 ataagatcca gctttcctcc gtgataagat tgcatgactt gatgttcact ccatgaaata
       61 tcagaaacat tataagatga tatatctatt ggattaaatt catttctcat acgatcatat
      121 aaaacatttg tttctgttgt atcaacaaaa agaattcctt ttttctccag tctgtcaaac
      181 atatcgtaaa tagcctgttc agcttgtgct ggtaatgatg gaatatccaa aagagattcc
      241 ccatttattt tattcattct aataccaata acatttccat tatcattata tattttctct
      301 gcactcccgg aaccataata ctgattgaaa caacgaactt cgcttgtcac ctcttcatgg
      361 ctttgagata tagtaaacat cttcaacact tttgttgtat cttccatatc ttcatacacg
      421 acagcattac cacctttacc aataacatta cctggcacgg gtgatttatt gttgctaacc
      481 ctcggcaact ctgattgagt ataatccgct ggaggtaact ccggctgcgc ataatccact
      541 ggaggtaaat caggtctgtt cgaatgaaca gagcctcttt ctatagtcgt gctcactggc
      601 gatgtattca gcatagcctc aatttttctg ctaatctctc ccttaggcca tcccaacctg
      661 tgcaacagat tagtaaaaca accactatga ctttctcttg taacgcaaaa cttattatca
      721 gtgaccacaa cacgatatgt tctgttgcca accgttactt gcgtcccgct atcagagtga
      781 acagcagcat cccttacaga ggataaaaca cgattatcag gcgaagtcag gtttctggtt
      841 aaagaattcc atgaacatcc caaatttata gaagagggcg ataacataca tttcaacctt
      901 caaaataaac ctatctaatt attcctaaca gacatccccc atacatgaca acaaaaaccg
      961 gagccggact ccggtttttg tgaagctatc gggttacttc atttcgccaa tattttccca
     1021 cttcccgtca gcacgcagga tttgcagcgg tcttactaca cactgacccg aggacgaaaa
     1081 aagagttcgg gctgggaaga gacagaagag tagcaatttc agaagcattc acaccaacat
     1141 tgagcttttc agcgaaagtg ggcacgaatc attgctggac agggttaaag gcactgactc
     1201 aatcactctt catacgtggc ttgatcgata tgaaacaatc ctcagctaga gggggagcaa
     1261 gccgaaaaca ttactcaact acgccagcaa aatcagggca attcgaagaa aattgccgga
     1321 caaaccgctc actgacatat caacgaaaga ggtggcagca atgctaaaca cctacgtcgc
     1381 agaaggtaaa gcggtttccg caagagtaat caggtcaacc cttgttgacg tttttcgagg
     1441 ggcaatagcc gaggggcatg tggcaacgaa tccagtaaca acaacccgtg cagcaaagtc
     1501 agaagtaagg cgctcaaggc tgacagctaa tgagtatgtc gctatttacc atgctgctga
     1561 gcacctcccc atctggctga ggctgtcaat ggatttagct gtcgttacag ggcagagagt
     1621 gggcgatttg tgcagaatga agtggtcaga cataaacgat ggtcatcttc acattggaca
     1681 gagtaaaaca ggagccaaaa ttgccattcc gctggctcta accattgacg cactcgactt
     1741 cgggtctctc tagtgtaatt ggacactgaa tagactctgg cgggcactgg aaatttcctg
     1801 aactaacagg aaacttacat tgagatattt tgtcctgaat actctcttgc gattgtgctt
     1861 cacctgagtc cgaaagcata ctgagcatcc tatcgcgaag agcttcaggg ccgttattaa
     1921 acccataaag gaataactca gtaagcaatc ccccactact gccgctggat agaaaacgcc
     1981 catcggtttc attaaataca atgctgacag tctcattacc taatgtaaac tcccctcgac
     2041 cgctagatgc tacttgagct cgaaggacat tcaatccatt aaaaatgagc ttgatctaat
     2101 ctccgaattt aacggcatta taaaactccc tattttaatt taaactccag acttaaatag
     2161 ctttaacaaa catctgcctt acatgacaac aaaaaccgga gccggactcc ggttttgtga
     2221 agctgtcggg ttacttcatc ccgccaatat tttcccacct cccgtcagca cgcaggattt
     2281 gcagcggtct taccacgcac tgtatctgct ttttatccgc atccagtatc accacctgcg
     2341 t
//
LOCUS       DAZUFB010000264         2307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZUFB010000264 DAZUFB010000000
VERSION     DAZUFB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.268"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            181..603
                     /locus_tag="ACHY3M_005249"
     CDS             181..603
                     /locus_tag="ACHY3M_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFS4319944.1"
                     /translation="MGKNEHVLVNQSTGEVTGTNVVTYKKVDSEEFVKLFSRNIALTF
                     DLTSSGIKSLNVLIWSVQNTAIQKDVVALDQLAFDDFMKENPNLKMTLRTFQRGLSEL
                     EASKIIAKTMRKGFYYINPNFVFNGDRIAFTTLIERDC"
     gene            679..963
                     /locus_tag="ACHY3M_005250"
     CDS             679..963
                     /locus_tag="ACHY3M_005250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFS4319945.1"
                     /translation="MTKHDAIRISQLHQIFYDDEGLIDSEKNVTILYGIGFTIDEIAY
                     FGNTTPGTVQGQLFNARAKLSCASASSLRPMILLRLLLLLIFEQDRLNGL"
     gene            1066..1569
                     /gene="ddp1"
                     /locus_tag="ACHY3M_005251"
     CDS             1066..1569
                     /gene="ddp1"
                     /locus_tag="ACHY3M_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HFS4319946.1"
                     /translation="MKQIKFRLTDEEYNVAHVNAISNGVNSVNAFAKRKVLDVSSTPS
                     KVIVSNEPSVPVKTCLYPHELELIKRNAALHGMSVSREIAIRLRKSVLKNEVCLYPEE
                     VQVLRKLSTTINRVGRNIHFIISGDRFCTVNDPDFRNEIKELTALCETVDKTIEQLVF
                     SVNNRFG"
BASE COUNT          700 a          451 c          519 g          637 t
ORIGIN      
        1 aaaaaaaaaa tgtcgcttag aggctcgctt atcggattga caacgacaaa aaacgcgctt
       61 attttgtcgt tagtggcgaa atcatgagag gtttttatgt cggtggtaag gtacaaagag
      121 aaccctttta taaaagattt agcgttaact atttcctcta aaagagtcat gatctcccct
      181 atggggaaga acgaacatgt gctcgttaat caatcgaccg gtgaggtgac ggggactaat
      241 gttgttactt ataaaaaagt tgatagtgaa gaattcgtta agttatttag ccgtaatata
      301 gcattaactt ttgatcttac ttcctcaggt attaaatcgt taaatgtttt aatttggagt
      361 gtgcaaaaca cggcaattca aaaagatgtc gttgcacttg atcaattagc ttttgatgat
      421 ttcatgaagg aaaatccaaa tttaaaaatg acattgagaa cgtttcaacg aggattatct
      481 gaacttgaag ctagtaagat aattgcaaaa acaatgagaa agggatttta ttatataaac
      541 cctaactttg tttttaatgg tgatcgaata gcgtttacaa ctcttattga gagggattgt
      601 taatatatac cgtcgcaggc gaacaaaagt ggtgttttgt tcgcctgcta atcacaacaa
      661 cctgcaagag aggttattat gactaagcat gatgctatac gtatttctca acttcatcaa
      721 atcttttacg acgatgaagg attaattgat tcggaaaaga acgtgaccat tttatatggt
      781 ataggattta ctattgatga aatagcttat tttgggaata caactccggg aacagttcaa
      841 gggcagctat ttaatgcaag agctaaattg agttgtgctt cagcttcatc tttaaggccg
      901 atgatcttat tgagattgct tttactccta atctttgagc aagaccgctt aaatgggctg
      961 tgatcaatgc aggatagaca atcatggtca aatgggtacc atttcccatg attgtccaaa
     1021 aacgtggttt ttgcctgtgc ctgccgcagg ctcttaaggg gtactatgaa gcaaattaaa
     1081 ttcagattga ccgacgagga atacaacgtt gctcatgtta atgctatttc aaatggcgtt
     1141 aatagtgtaa atgcttttgc aaagaggaag gttttagatg ttagctcaac accttcaaag
     1201 gttatagtat cgaatgaacc atctgtacca gtaaaaacat gcttataccc acatgaactg
     1261 gagttaataa agcgtaatgc tgcattacac ggaatgagtg ttagccgaga aatagcgata
     1321 cgcttacgta aatcagtttt gaaaaacgaa gtttgtcttt atcccgaaga agtgcaggtg
     1381 cttagaaagc tttcaactac gattaataga gttggtcgaa atattcattt tataattagc
     1441 ggcgatcgtt tttgcacagt taacgatcct gatttcagaa atgagataaa ggaacttaca
     1501 gcattatgcg aaacagtcga taaaacaata gagcaattag tttttagtgt taataatcgt
     1561 tttgggtaaa aacgtattat caagaaccga cagtaggtca ccgggcaaac gttgcagaat
     1621 tgcgtcagag acgtcatttt gcggcgtttg ccctatcctg catcggagtt aaaccactaa
     1681 gatttttatt ccccgttctt cggaacgacc gagccgccac ggttccggca gcgccttgag
     1741 cgcaaccgga aaccgtctgg ggagaccccc agacccccat tgctgtaaca ccgacggtct
     1801 ccgaccacct accaaccgcg tggctgcctg taccgtgcaa atgggctttg cagggggctg
     1861 acgcggttcc tggcgacctg aaagcccctg cttaggggta tcggcgcgcc tgacctcctg
     1921 agagcctctg taagcgtttt tcgcgccttc catgccctga cgggcattta aaacccgttt
     1981 cgtgcatctg agagcttctg agagcgtttg aagagctatc tggcgactca tccccaaaag
     2041 aacaacagcg gaatcaccga taacatgcga gaggtggcac cgctcgccgc agccccagcg
     2101 accgaacgtt agtgagcgag tgggcgagga agcggaagag aatcgtctgg tcaaaaaagc
     2161 acctacgctc ggtgtagaat aagaactaag aagggaattt cttgtaaccc gcatgaatgc
     2221 tagatcgtat ttgcacgatc acgcgttcca aacgacagga tggcacgtct ataacgacaa
     2281 tttaatttcg cgataaacga caaaaat
//
LOCUS       DAZUFB010000265         2296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZUFB010000265 DAZUFB010000000
VERSION     DAZUFB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.269"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..130)
                     /locus_tag="ACHY3M_005252"
     CDS             complement(<1..130)
                     /locus_tag="ACHY3M_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319947.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLI"
     gene            complement(417..1235)
                     /locus_tag="ACHY3M_005253"
     CDS             complement(417..1235)
                     /locus_tag="ACHY3M_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS4319948.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1387..1758)
                     /locus_tag="ACHY3M_005254"
     CDS             complement(1387..1758)
                     /locus_tag="ACHY3M_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS4319949.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1748..2119)
                     /locus_tag="ACHY3M_005255"
     CDS             complement(1748..2119)
                     /locus_tag="ACHY3M_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4319950.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2132..>2296)
                     /locus_tag="ACHY3M_005256"
     CDS             complement(2132..>2296)
                     /locus_tag="ACHY3M_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4319951.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          762 a          576 c          393 g          565 t
ORIGIN      
        1 gtattaactg gcagcgttcg cgtgaaaggt aagtattctg cgcaatctcc ccgactgtcg
       61 ccggttcggt gacgcttaat tcatcaaaca caactctggc ggtttctgtc atatcctgct
      121 gtttcagcat gtctttttac cctttccggt taacgtgaca caccaataac tcttgccgaa
      181 aaagccagca agctgaaaga cctgtattaa taactaccag cacatttaac gcactgcgct
      241 actttgcggg cacaaaaaac ccgctcagag gcggggtcaa gctatgcggc gaaataacca
      301 ctcttaacag catacctgat tttttacgta cgtaaatgct ttgccgtgca tatttttcat
      361 gcaaatgtca cgtcctgcta tttttcagtc ttataaattt aaaaccatag aaaaaatcaa
      421 ttatgtttta aaaatggata ggtaaagaat aacaagtgac acagattcaa accaaaatgg
      481 aaaagggtgg caacccacaa acgcccactc cacatccatg acaatccata cacaacacca
      541 gataacgtgg caaataatac aagtaaagca ccacctgaat agtgataaaa accaaacaac
      601 aaagccgcca caattaatgc aaccaatgga gacgttactt ctgatagccg tgattgaata
      661 taccctctaa ataatgattc ctctgccaga gacacaaaaa acaaattagc caatataaac
      721 tctggcaacc actcaggaaa atgaatctct ggctttaatc caccaaaaaa aacagccaaa
      781 aacaggataa gaggaacaga gagcgacaga gccccccact tccacaaaga cacttctgat
      841 tttacttctt ttttaaacaa agaagatgta cacaggacta acaaaaatgg caccagcgct
      901 ttatcaaaat taaaatacat tgtataggga gtactttgag ggccaacagt aacagaattt
      961 agcactacag gattgtgaaa ccctggccat agatggaaag ataatgctat ggctgataaa
     1021 actatgccaa cttcatatat agatttagcc caggcgttat atttccagtt gaacttcaaa
     1081 ataataaaaa aaacgattgt agcaacaaaa aataacaccg accaatcaat aatatcatta
     1141 agcacagcca ggacaacaga caccgtcaac aatgaaaaag ccactacctt atgccaactg
     1201 agagttgaca gtgacagtac taacacaatc cacataagca ctccttttat ttaatgatga
     1261 agattgatta tcaatatttt caattcacca ggcaacattt tatctacctt ccacaataca
     1321 tgaccatcaa aaaataaaca tgttaattat aaacacagaa aacataaccc tcatcactat
     1381 atatccctac cgcatatcca tatctaaccg gacattcaga gccataagca ttccttcaat
     1441 aataccttcc gctttataaa gccttttacc aataagccca tcagaacatc tatgcttacg
     1501 tgcaagagcc ataaatgtca ttccacctac gtaataatcc accaacaaat cgtgcaaata
     1561 ctgattattc ttgtttaatc gtgccataca accacaaatt atcatggcgt catcatcaga
     1621 acactgagga cgtgatttca ctttagtcgg gattaatcct ttaaaaccag cagctatcga
     1681 gggccatgtt acatcttcat gattatttgc tgcccatgcc ccccatcgct caagaaccat
     1741 ctggatatca cgcgccatcg ttaccacctc tgatttcgta aatcttcacg cccagccgtc
     1801 cacctggcac aggctgaccg cgcacaatat tgatttcatc aaactgctcg tcatcaatga
     1861 gcacttccgc atgcgtcagc gcatccagcg gtgctttcag aatgttgtcc aggtcacggc
     1921 ggcgcttatc cggtggttct gcaataattt ttattgccag ccgtccggac aggcttaatt
     1981 tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgctca ccggcttttg
     2041 atacaaaata tgtgctgcca cgacgtcgcc agtaggtgtt caccgtcggc gggtaaggca
     2101 aaacaaactc tatacgcatc agtaacctct tttacccgag cacgccggtt gcaaaagcgt
     2161 gatcaagaaa acgaaaaatt aaatcaacct gggaaccatg cttttcttcg aacgccagcg
     2221 gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa atatcgtggg
     2281 attttgttcc cattcc
//
LOCUS       DAZUFB010000266         2274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZUFB010000266 DAZUFB010000000
VERSION     DAZUFB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.270"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..482)
                     /locus_tag="ACHY3M_005257"
     CDS             complement(<1..482)
                     /locus_tag="ACHY3M_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4319952.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHVSTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
     gene            complement(553..>2274)
                     /locus_tag="ACHY3M_005258"
     CDS             complement(553..>2274)
                     /locus_tag="ACHY3M_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4319953.1"
                     /translation="PEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQ
                     VLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRA
                     VNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKR
                     IADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDD
                     ASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLED
                     QSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLA
                     ADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLT
                     AKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGN
                     TETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKN
                     GVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKA
                     ASGISIR"
BASE COUNT          485 a          666 c          543 g          580 t
ORIGIN      
        1 ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc
       61 gttttcccct tgttgtcagt tacgcggaga taatccccgg agaaagtcga cacacggctg
      121 taagccactc ccgccatcgc atacgcgctg aaccattcat tcacgcgcac agacggcccc
      181 gccattacgc tgaaccagcg gttacgcacg gaatcttcat gccagcgggt atcgctgtaa
      241 cgggtaatct ggcgattcct gtctcctgca tagctgaatg acgtcaccag ccccagcgtg
      301 tccgtgaatt cataacggta tttcacgtta atcccgttaa gattatcgct accgggagcg
      361 ttcgtcgaga catgaagata ccccgcgctc agcgtggcct gctgctcaga cgcccatgca
      421 ggcgcaccgg atacggtcag acaaatggct gcggacaaaa tggcggcata aagtttacgc
      481 ataattacct ctcgcttttc tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg
      541 gttataaata tttcaacgaa tactgatgcc ggaagcagct tttttggtca caatcaccgt
      601 acagtcggtg atattgcctg ccccctgatt gcctttctgg aaaatcttaa actccagagt
      661 gacgctacca ccaccactag gcatatcaat aactgcactg taactaccgg gaatggcccc
      721 tttagtttct ctggatgcga ttaatacgcc gtttttgcga acttcaaaac cataccccgt
      781 gtatcgcgtg cctcctgggt tatttccgct ccccggatcg tcatacgcta taccgttaaa
      841 aataatgggc ggaataataa tctggcggtc aaagttatga tcatcgctga tggtgactgt
      901 aaccgtaccg tttggtgttt ccgtgttacc ccacgtaccg acttttttcg ggaaggcttt
      961 tgatacagct ttaacgaaat cccctctgac ctgggtcgcc tccagcatgc ccttaatcgt
     1021 acagttctgg ttaatcgtga cattgttgag cgctcctgag ttcgcattca cactgccact
     1081 gatatccgca tttttcgccg tcagtcgccc gtctggtgtc agggaaaatg ccggaggatt
     1141 accgccgctg gtaatggtgg gagccgtcag atatttcagg aacacttcat tcatgaatat
     1201 ctgatcgccc tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc
     1261 aatcctgtct gctgccagca gcacctgact ctgcatgccg tcgggggtgt tctcaatacc
     1321 ggcaccgata cccgcaatat aaaggcgtcc gtcctgcatc tgctgcagct tcactgccca
     1381 catgctgttc aggttattat ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc
     1441 actctggtct tccagtttct tattgacggt ctgtgttatt tcattgctga catccgttat
     1501 ggacgtcctg atttcagtca ggtcaggcgc aagctgaccg ttatcaatct gcgtccacag
     1561 ctcctgagcc agatgggttt tccctatctc gcctttgaaa aaatccagat agcctgatgc
     1621 atcatcactc ggctgaccga cagcctctac aaatgccgat ttgccaacgg tgttcacact
     1681 gcggatataa aagtaataat catggcccgg tttgatattg atactggcag ctatccagta
     1741 cagcgccgtg ccaagatagc gggctgtggt ttcaacctgc ctgatatcag caatccgctt
     1801 ttccgagaac cagaactcaa actgtaccgt cggatcataa accgcaagat gcggcgtggc
     1861 ggttatctga aaatagcccg gtgtcagctc aatccgagac ggcgctgccg gtgcggcaat
     1921 ccggaacgat accgatgccg gatcgccctg ctgtccccac gcatttaccg cccggactgt
     1981 caacctgtag ttccccggcg ccagttgcgt gaagcggtat gtggtttctg tcgtcctggc
     2041 cgtgctgacc agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa gcaggaagct
     2101 cacgcccttc accaccttcg gcgtgtccca gcgcgccagc acctgatatt ccccgctgtc
     2161 tgcggtgact tcggcggtca ggtgctgcac cgctggcggc gtgacaccat tcaccgtgcc
     2221 gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg gcttcttttt ccgg
//
LOCUS       DAZUFB010000267         2267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZUFB010000267 DAZUFB010000000
VERSION     DAZUFB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.271"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..609)
                     /gene="ymfL"
                     /locus_tag="ACHY3M_005259"
     CDS             complement(58..609)
                     /gene="ymfL"
                     /locus_tag="ACHY3M_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFS4319954.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPELEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(602..862)
                     /locus_tag="ACHY3M_005260"
     CDS             complement(602..862)
                     /locus_tag="ACHY3M_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4319955.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNTATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEISRG"
     gene            960..1652
                     /locus_tag="ACHY3M_005261"
     CDS             960..1652
                     /locus_tag="ACHY3M_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4319956.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDLKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
BASE COUNT          615 a          544 c          523 g          585 t
ORIGIN      
        1 gttcagcgac gctgaccacc agctgttgcg acaaacggtt atttgccgtt aaactgttca
       61 tgcgttagtt tctccacaga cacaaaacgc cacgacgccc ggagctgcac actcgcgggc
      121 gtcactcttt tctggagcgc aaaagatttt gtagaccagt gctgcatgct cctggagctt
      181 cgaaattgac agatacaact catcattaat tgctgtctgc tcgtgtggct ccactacccc
      241 atcttcgatt gccgaacgaa tctgctttga gtaactcccg atctgttcga tgacttccag
      301 caggcgctgg tttatatcgg cgttctctac ttcctcaagt tcaggaagcg atacaaacac
      361 cccaccagca gactgtgcga cagcatccgc aatgtagtga gtgccagccg cgcgctgtaa
      421 aatcattgcc catcccagcg ggaaaatctg atcgccatct gcacgaaggc ggttgaataa
      481 agcgttctct gttacatcca gccactcagc agcttcagcg taaccccccg gcaacgccgc
      541 gatagttttt ctgacagctt tcacgtacca ctcaggctgt ttttccactt tccagtgatg
      601 cttacccacg gcttatctcc tgttcctgtg gtttaaaccc attctggttt tggctagatt
      661 gaaaacgtgc cggataaaga atctgcattt cgctgatttc acccttaaaa aaattggcca
      721 gacgttctgc aagatcgata gatggaattt gttccagtct ttcaatacga ctcagcgtcg
      781 ctgtattgac ctgaacgccc gcagcaacat gctgcaaagt aaatccgtgc gccttacgca
      841 cattccgtaa tggtgattgc atataacctc cacatattgc gtgatgagca tattatttca
      901 cgcaaatatt ttgcgcaagt tgatttgctt aacgcgcaat aaagaaatgt aataaacgca
      961 tgaacatagg aaatcgagtc agacaacttc gccaggcgaa gaacatgaaa atcgccgatc
     1021 tcgctgaagc aataggagtg gatgcggcga atatctcgcg cctcgaaaca ggtaagcaga
     1081 aacaattcac tgaacaagcc ctgagtaata ttgccaggag cttaggtgtt gatattgctg
     1141 atctctttac ctcagacctc aaaagtaata ctgtatgtaa aaacagtatt agtgaggatg
     1201 ttgcgcaggt gaaggatgta ttccgtattg aaatgctgga tgtcagtgcc agtgcgggaa
     1261 atggccttat ccagggcggt gatgtcattg atgtgattca tgccattgaa tacagaactg
     1321 ataatgctgt atcgatgttt ggtggacgac cagcaaatca cattaaagtt atcaacgttc
     1381 gtggggacag tatgtgtcca accattgagc caggagatct catcttcgtt gatatcagta
     1441 tcaatcagtt tgatggggat ggtatatatg tatttggttt tgatgataaa atttacgtca
     1501 aacgactgca aatgatacct gataaactgc tggtaatttc tgataatcag atttaccgcg
     1561 aatggggaat taccagcgaa aacgaacatc ggtttatggt ctttggaaag gtcttaatca
     1621 gtcagtcaca aacccttaag cgacacaatt aaccccctac ctcaacatca attagccacc
     1681 agaaggtggc ttttcattac ccaccaaatt gcatatctcg caataaaaac acttgcatga
     1741 tgcgcaactt cattttatct ttctttccag acctacaaac aaggtactaa caaaatttgg
     1801 ttgtaacacg gcgtatggca catgcgtcgt tagcggtctg gggacgttaa aggggacaat
     1861 ccactccttg ctcgggcaaa caaaccaggt agccggaatg tgcaagtcaa tgaggatgct
     1921 gataagacgc ctaaccagcg tggcgattcg gtttgacgcc tgggaagaga ccagggtgca
     1981 acgatgaggg catttatgga accgcgacaa agtgtggtgc cgtaactggc taagtgctct
     2041 cagcgttgtg gtaatccgcg aaatggcgcg gcggtaagta tggcggggtt attccttccc
     2101 cgttgaggac accgggttgt caggttgacc atacgcttaa gtgacaagcc caccacaaca
     2161 gccactgctt tggcggtacc agtttgtaca cttgcttccg gctggtaccg ctctttttac
     2221 aaaacagaga agagcatcac cggacgacgg gctcataacc caatcca
//
LOCUS       DAZUFB010000268         2229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZUFB010000268 DAZUFB010000000
VERSION     DAZUFB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.272"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..338)
                     /locus_tag="ACHY3M_005262"
     CDS             complement(<1..338)
                     /locus_tag="ACHY3M_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319957.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTI"
     gene            complement(600..2207)
                     /locus_tag="ACHY3M_005263"
     CDS             complement(600..2207)
                     /locus_tag="ACHY3M_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS4319958.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTRD
                     MILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSRI
                     NKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCHYDSERKVLTSHHGI
                     IRDEENACYCLGALQVPFKQMKNPTDPEELANIFNKKHKEHMNDPLLHLIRSNIMKPT
                     AVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKYGHHGVGVDQNQQFDNGVM
                     GLNSTKKVLDKNNKIIYSSGLSCFQSH"
BASE COUNT          694 a          413 c          351 g          771 t
ORIGIN      
        1 ttaattgtca caataaactg ataattatta tccctacgta gaatatgagc gcgcatgccc
       61 gatagcatac tatatttttc tgaatgattc gtttgtatgg taaatgaaat tttgtcatct
      121 cgaatagatt gttggaaacg ttctatttgc caatcatcaa ttttttccac aacttcaccg
      181 agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat atacccaaac
      241 agttctttag tgaagttagt aacatgctct tgcaccatat catttgttgc gattgtcgtt
      301 gtactgtcgt tagtgcctgc cacagttcca attttcatct tttacctcat ataattcacc
      361 gcgaggaatg catagttaac atttaatctt atgacataca cctcatgttc gatggaaaaa
      421 taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt cttatctcat
      481 agcagagaaa gtccctaaag attgtagagg catcatcagt acatacctct acaatcgatt
      541 aagaaaacct atgaaatact ggcaggatta aaaaggcata ccaccgtatt catggagttt
      601 taatgagatt gaaaacaact aagccctgag gaataaatta ttttgttatt tttatcaagt
      661 actttcttcg tggaatttag ccccataaca ccattatcaa attgttgatt ctgatctacc
      721 ccgaccccat gatggccata tttttgtcta atgtatatgc tgggatcttc acatttgagt
      781 gtctctccac atatgaagat acgttcaggt tttggtctga aatcagtagt gttattaata
      841 taatcagcat atacagctgt gggtttcata atattgcttc ggattagatg taatagagga
      901 tcattcatat gttctttatg ttttttatta aatatatttg ctaattcctc aggatcagta
      961 ggatttttca tctgtttaaa tggaacctgc aaagcaccga ggcaataaca tgcattttct
     1021 tcatctctta ttattccgtg atgactggtt aaaactttcc tttctgagtc ataatgacaa
     1081 acatcgagcg ttttaattaa gttatacgta tcaccggcag agagtttatg atttgctagt
     1141 cgtgcagtat agttaccatt aaaattgatt tcatgatttc cagccagaac gacgacattt
     1201 ttattaatac ggctatcttt attactctcc atatttcgca tagaatttag caaagtaagg
     1261 atatatttat caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata
     1321 caaggggtta tcgcatcgtc ctttaaaaaa acatgtttgt gcaactcatt caatatcatg
     1381 tctctcgtat ttaataactc gtagagcact ttattatttt tatgcttgta tgcagatgaa
     1441 gccatgttct cgtgatttaa taattcacat aataaactcc aacccttttc cctgatgcct
     1501 atatgccctg aaacgagcaa tgcataaagt acagcgccta ctcttccatc tgtatcgcca
     1561 aaataagccg tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt
     1621 ttagaaaaca cagatttaac atagccagca aacttatcct taggaaacgt ctctttagag
     1681 acatcccaac gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt
     1741 atgatagaaa gcccattaac tttataagtt atgtctgatt tatctccaga ggaattaata
     1801 aaataataac cgcccttaca ctcaacagta acggtaatgg gagacgataa ttttaacaca
     1861 tggttattgt ctggtataat tgtaaagata aacgtgtcag tagtattgtc ggcgaatcta
     1921 ttatattctt gaatatctat agaaggttca ctattgtaaa accatacttt ctccatctga
     1981 tgactacgat catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca
     2041 aaatcgttat ctatttgcga atcattatcc agtttatcaa tttcaactat ctttgaacga
     2101 tgtgttttat caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct
     2161 ttatggtttg aagggacgta cttaaatata ttgtcaattg ctttcataaa taacctgcga
     2221 tatttttga
//
LOCUS       DAZUFB010000269         2211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZUFB010000269 DAZUFB010000000
VERSION     DAZUFB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.273"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1784
                     /gene="espN"
                     /locus_tag="ACHY3M_005264"
     CDS             <1..1784
                     /gene="espN"
                     /locus_tag="ACHY3M_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFS4319959.1"
                     /translation="LNARYDLIRDILRYSLNIRQGIKHDDVNRIAENIIKKYGITEGM
                     NPKPRNARISKELLLLAVDRQIEWAKKHFITKDALENVVSKCDLSSIFNVNKVLQNTI
                     LEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLS
                     GLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHS
                     AIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHP
                     SNNANFYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGG
                     FYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADG
                     TFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVP
                     TPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAP
                     DEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDK
                     TAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          734 a          327 c          462 g          688 t
ORIGIN      
        1 ttcttaatgc cagatatgat cttattagag atattcttcg atattcttta aatatacgac
       61 agggaattaa acatgatgat gttaatagaa tagcggaaaa tataataaaa aagtatggta
      121 taactgaggg tatgaatcct aaacctagga atgccagaat atctaaagaa ttgcttttat
      181 tagctgttga tagacagatt gagtgggcga aaaaacattt tataacaaaa gatgcattgg
      241 aaaatgttgt gtcaaaatgt gatttatcat ctatctttaa tgttaataaa gtgcttcaga
      301 atactattct tgagtttgtc catgaaatta ataatatatc atctgctcgc tggatgtcaa
      361 aatcagaaaa gaataataaa caaaaagagg caatagaaaa gttcaaaaaa gaagtatccc
      421 atatgaatgg cgggcagcag tttatttggg ggtttgataa ggttattcaa gaaggcttaa
      481 gtggattgat tgagttaagt atcgatatta atgatagtac aaatcatcgt gataagtctt
      541 ctctttctcc tgatgggaga gctgtgttac attttttagg tacaatttgg aatatggcga
      601 tgggagctgt acctggttat aatgcattgt ctggtgtaag tagcatttta catagtgcta
      661 tagtaaaaga atctagcaat atctgtgatt atattcaggg ggctgtacgt attggaatgg
      721 actttgttcc aggcactcgc tctgacttac atagccgttc gctgcagata aaatatgagg
      781 ctttgaagca tatagaaaaa aacattaatg ataatattat ttatcatccg agtaataatg
      841 ctaatttcta ttctgtaatt gagtcaattg atggtaatga ctttatatat aacgaaaaac
      901 aatctaaaat attagaaatg aaacaggatc gtggggggaa tagatatagt gcagtagatc
      961 ttaactcttc taagtatggg tattatgaga aagttggcgg tggtttttat agatatatag
     1021 aatcctttaa ccccatatct tcagagacac caaataaaat agtctacaag ggggaatcag
     1081 tagatttaac taaggagcca aattcggaat tatattcagg taggtattct ataaataaca
     1141 aacaggttaa tgtttatttc tttcgtgacg ctgatggtac attttataaa tcagaaggtc
     1201 ttcatggtgg gggagttatt agatacatag ataaaccgta ttctcagtta agagaaggag
     1261 atattgggta tgatgaggat ttgttggata tatacgatga ttctccggtg cttgaagaca
     1321 cgttgcctgc tttatcttct gaaatagtac caactccaga acatagtatt aaacaaattt
     1381 attcgaaaat taaggagggg cacatagaac tgtccgattc agacatcata ttgtgtcgcg
     1441 gcacaaccgg tattcaagct gaaaatatcg ttgaatataa aactgctgga gggcttcctg
     1501 attcaaatcc aaatgtaaaa gcaccagatg aatatatggc acaacagcag gtacgtattg
     1561 gaagaatatt gcctgaatac acatcggatc ttagcgttgc tgatcggttt agtcgtgagc
     1621 attatctaat agttgttaaa gtaaaggcaa aatatatcac acgaggaagt gttacagaga
     1681 gtggttgggt tatagataag accgcacctg ttgaaccact tgcgataatt gatagaactt
     1741 ttggtatgaa ggaaaatatc tcaatggtaa atgcatcgaa atagtttttt tacaatctat
     1801 gtcctgcctc ctctggtaaa aacgatgcta catcttggag atgttgcacg gcagtacggt
     1861 gttgaccgat atatagtgaa gtacacatcg gtcaacgaat taccattgtc agcaatatca
     1921 tcctgataaa ctcagtactc gtgagccgct tgatgacggg cggaatagcc caaaaggtaa
     1981 acgatccgca tggcagtccg tcggaaagct gctgcttcat acgaccgctt aaaccgtcag
     2041 ttagtgtcag tatcgctgaa gatcagcttc ttttgctgat ttactctgtt tttacctgcc
     2101 ctgatgaaat actctttaat cataacattg attatatcga aaatatattt tttgctatca
     2161 ttaagaatat ttatatgtgg aaccggagta ttttggacgg tagtctttcc a
//
LOCUS       DAZUFB010000270         2202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZUFB010000270 DAZUFB010000000
VERSION     DAZUFB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.274"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..155
                     /locus_tag="ACHY3M_005265"
     CDS             <1..155
                     /locus_tag="ACHY3M_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFS4319960.1"
                     /translation="NYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGS
                     NWTFQE"
     gene            169..409
                     /locus_tag="ACHY3M_005266"
                     /pseudo
     CDS             169..409
                     /locus_tag="ACHY3M_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            429..760
                     /gene="sctI"
                     /locus_tag="ACHY3M_005267"
                     /pseudo
     CDS             429..760
                     /gene="sctI"
                     /locus_tag="ACHY3M_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            757..1491
                     /locus_tag="ACHY3M_005268"
     CDS             757..1491
                     /locus_tag="ACHY3M_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFS4319961.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            1507..2088
                     /locus_tag="ACHY3M_005269"
     CDS             1507..2088
                     /locus_tag="ACHY3M_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFS4319962.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          743 a          364 c          421 g          674 t
ORIGIN      
        1 ataactatgt tgacgaatat tataaacaat ggggtaaaca atatgtaaga tttaatgtga
       61 atttgaaaaa tcaggacaca aataatagtt catttagcta cggagataac cgattcgaga
      121 agtctcaagg tagcaactgg acatttcagg aataattgag gaggacttat ggcagattgg
      181 aatggttata ttatggatat cagcaatcaa tttgatcagg gcgttgatga tctgaaccag
      241 caagttgaaa aagcgttgga ggatttagcc aaccaatccc tccgacacga aatttcttgc
      301 cgaatatcaa agtgcattag ctgagtatac attatatcga aatgcgcaat ctaacgttgt
      361 aaaagcgtat aaagatcttg attcagcaat catacaaaac ttccgctaaa ctggataagg
      421 attttattat gtcagtaagc aatatgccac ctattgatag agctgagcaa tcaactgctc
      481 atgaaataca gcaagcgaaa gtgattgatt taaatgaccg ggttctcaat cttgacaacc
      541 cggatgataa aatgatctcc gcttttgcca actatgctgt acaaactgaa aattggcagc
      601 aaaatgcgtt acaagcatta aggtctgata aagagggggt aactcctgaa aaattattag
      661 tcttgcagga tcatgtgctt aattataatg tcgaggtttc attggtggaa cattagcaag
      721 aaagattgtc gctgcagttg agacgttgac ccgctcatga aatatatatc attattattg
      781 tttatcctgc tgttgtgcgg ctgcaagcag caggaattat tgaaccatct tgatcaacag
      841 caagctaatg atgtccttgc tgttttacaa agacataata ttaatgctga aaagaaggat
      901 caaggcaaaa caggattttc aatatttgtc gaaccaacgg attttgcttc tgcggtggat
      961 tggctaaaaa tctacaatct accgggtaag cctgatattc aaatatcgca gatgttccct
     1021 gcagatgcac tcgtttcatc tccacgagcg gaaaaagcaa ggctatattc tgctattgag
     1081 cagcggttag agcaatctct caaaattatg gatggcatta tttcttcgag agtacatgtt
     1141 agttacgatg ttgataatgg cgatagtggt aaaactgctt taccaattca tatttctgta
     1201 cttgcagtat atgaaaaaga tatcaaccca gaaataaaaa taaatgatat caagagattt
     1261 attgttaata gctctgcaag cgtacaatat gaaaatattt ctgttgtatt gtcaaaacga
     1321 agagacatta ttgagcaagc accaacatat gaaattagcg aacctgtttt tgcttatgat
     1381 aaggctatgc cggtttctat cttacttgca ttaatatccg tagcgacttg ctggctgtta
     1441 tggaagtata gggccattct aactaattta gtcaggttga aaattaagta gacatatgag
     1501 agcgttatga atcttgccct aagaaagatt atatatgatc caattagtta tattcatcct
     1561 caacgagtaa gcttgaataa tacacctatt aacaatccgg tgcttagatc tattaccaat
     1621 gaaatgattg ttctgcagta caatctttca gttgaacatt ttaatctgaa tagttcactt
     1681 atttactaca taaacaattg gaatttattt ccattgttct gtttattcag tggttatcac
     1741 ttttatcgag aaaggtttgc agaacgcgga tttttttata aggttcctgc tgtgctaagg
     1801 gattatttat cagctatacc ggtgaaaata aatgaaaagg cacgatacaa acccggcatc
     1861 gctagctatc acaatatcat cacatgtggt ttttcaacgt tgtcacccta tataaggcaa
     1921 caaccattgg caatgcagca acgatttaat ttattatttc ctgattttgt tgatcacata
     1981 caattgccgt taccacttgc atcaactctt ttagagagaa ttacatttta tgcgaaaaaa
     2041 aatagagatg agcttgataa aatctcctgc aaatggtgtt gtgattaaaa gaaaaatttc
     2101 tgatggattg aaataaatag tttctcttaa agaaagtatc cttcttgaaa ccagcgcaaa
     2161 aattcagagc attgaggtaa agagagaaga aaaatttatt ca
//
LOCUS       DAZUFB010000271         2200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZUFB010000271 DAZUFB010000000
VERSION     DAZUFB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.275"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..229
                     /locus_tag="ACHY3M_005270"
     CDS             23..229
                     /locus_tag="ACHY3M_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4319963.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(485..757)
                     /locus_tag="ACHY3M_005271"
     CDS             complement(485..757)
                     /locus_tag="ACHY3M_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319964.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            917..1450
                     /locus_tag="ACHY3M_005272"
     CDS             917..1450
                     /locus_tag="ACHY3M_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4319965.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1671..1784
                     /locus_tag="ACHY3M_005273"
     CDS             1671..1784
                     /locus_tag="ACHY3M_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319966.1"
                     /translation="MKGIEVETPASLDLIRAAAFAIRIVAIAVLVWAIRWW"
     gene            1786..2139
                     /locus_tag="ACHY3M_005274"
                     /pseudo
     CDS             1786..2139
                     /locus_tag="ACHY3M_005274"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2096..2191
                     /locus_tag="ACHY3M_005275"
     CDS             2096..2191
                     /locus_tag="ACHY3M_005275"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4319967.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSENG"
BASE COUNT          571 a          452 c          608 g          569 t
ORIGIN      
        1 cggtgacgga gacatacatc agatggaaaa aatcacaacg ggtgtgtcat acaccacgtc
       61 agcggtgggg acgggatact ggttactgca gctgctggac aaagtctctc cgtcccagtg
      121 ggtggcgata ggtgtgctgg ggagtctgct gtttggcctg ctgacgtatc tgactaacct
      181 gtatttcaaa atcagagagg accgtcgtaa ggtggcgcgg ggagagtagt cgatgaataa
      241 acaatacgaa ctggttgtaa aatgaatatt tctaactgaa aaaacgttcc atgaggtaag
      301 aaaaggtcac aggcaatcaa taacaggacg tgatgaaaga cccttgcatt tgtgcgcttt
      361 ctctttagat agcagcagat actgaaaatc tgagttgtcg gggagtcagg gatacagctg
      421 tgcaagagtt ggtcattgtg attccattga aatcctgtat gccatgaagg gcaggatttt
      481 atggctacct gagctttggt gatagtaagt tgaaaattcg cattttttgc tgacatgcgt
      541 aacgagaatc ccataaacag ggaggactta attcttcatt aacccatgcg ttgatattat
      601 gtttcagccg ttgaagcatc agcggtgtta atgttgtggt aataatatcc agcgttttat
      661 gtgagatctt accgtaaggg tctgcaagaa tgctgcttgt tgcttcgtta ttatctgcca
      721 tcagaagaag taactctgat ttaacgtttt ctgtcattag ttgtaaaaat cttctgcgca
      781 aactttcttt actgttcatt tatatggctt catttgttgt aatctgctgc gtctcaaggg
      841 atatgtttat gagagcgacc atgagtgttt gattatatac ctaacatatc aagggattag
      901 aaatcgataa atccccatga acgaaaaaat aaaatacggc ctgtcggctg ccgttctggc
      961 gctgattggt gcaggtgctt ctgcgcctga aatcctcgac cagtttctgg atgaaaagga
     1021 aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct gccgtggtgc
     1081 cattctggtg gatggtaagc ctgttattcc tggcatgaag ctgtcaaagg aaaaatgcga
     1141 ccgggttaat gccatcgaac gtgacaaggc gctggcatgg gtggagaaaa acatccgggt
     1201 gccgctgacc gaaccccaga aagcggggat cgcgtcattc tgtccgtaca acattggccc
     1261 cggtaagtgc ttcccgtcga cgttttataa acgaattaat gcaggcgatc gaaaaggtgc
     1321 ctgtgaggcg attcgctggt ggattaagga cggtggcaga gactgccgta tccgttcaaa
     1381 taattgctac ggtcaggtct cacggcgtga ccaggagagc gcgctggcgt gctgggacat
     1441 cgacagatag cagaatattt tcctgaaaaa tgacgttggc caacgcgggt ggataacacg
     1501 aaatcctgaa aactggtaaa acctaagtga ataaaagtaa aaaacccgtt tgttggcagc
     1561 aagcggggtt ttgtgttttc tgaccttgag taaggcaagg gagaaattat gggtagggag
     1621 gtactttccc tgtgaggaag tataaaagat tctttctgag gttgtccatt atgaaaggca
     1681 ttgaagtgga gacgccagcc agtctggatt taataagagc ggcagctttt gccattcgta
     1741 ttgtggccat tgctgttctg gtctgggcaa tccgttggtg gtgatatgaa ccgtgttctg
     1801 tgcgtggtta tcattgtcct gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat
     1861 taccgtgatc acgccatcat ctacaaagag cagcgcgata aaaaagccag tgagctggag
     1921 ctggcgaacg cgacaattac tgatatgcag atacgccagc gtgatgtcgc tgcacttgat
     1981 gccagatact cgagggaatt agccgatgcg agagctgaaa atgaaactct gcgtgctgat
     2041 gttgccgctg gtcgtaagcg cctgcggatc aacgccacct gctccggtac cgtgcgtgaa
     2101 gccaccggca cctccggcgt ggataatgca accggccccc gactggcaga caccgctgaa
     2161 cgggattatt tcatcctcag agaacggctg atggcaatgc
//
LOCUS       DAZUFB010000272         2192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZUFB010000272 DAZUFB010000000
VERSION     DAZUFB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.276"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHY3M_005276"
     CDS             complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHY3M_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFS4319968.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTAVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSY"
     gene            1215..1433
                     /gene="tdcR"
                     /locus_tag="ACHY3M_005277"
     CDS             1215..1433
                     /gene="tdcR"
                     /locus_tag="ACHY3M_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFS4319969.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            1689..>2192
                     /gene="yhaB"
                     /locus_tag="ACHY3M_005278"
     CDS             1689..>2192
                     /gene="yhaB"
                     /locus_tag="ACHY3M_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFS4319970.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILF"
BASE COUNT          707 a          445 c          402 g          638 t
ORIGIN      
        1 ataagatgaa tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata
       61 atttttcgac cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt
      121 aataaattga tttgaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc
      181 agcattgaga acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat
      241 actgatgcca tttctttgta acgtagtaag aagctcgctg tagtacccca tattggtttg
      301 tggcaacacc cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg
      361 ggacttactg gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg
      421 aagcttcatt tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc
      481 cggtaagaac gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc
      541 tttgaacttg ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa
      601 tgagacttcc accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat
      661 ttcacgggta atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac
      721 agcggtattt ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat
      781 tttactgacg gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact
      841 tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat
      901 aacgacctac gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg
      961 atatgtgcga ccactcacaa attaactttc aatactttcc atggtatcgt tacgcaaatt
     1021 aaataccttt aaatatcaac aagttaaagc ataaaaatca gcataaaccc tgatttttgt
     1081 caaaaaaaat gacggggata accatatttt atggcgataa catcattcgt tatgtggtta
     1141 atataaaaaa ggggctgaga ccatatttca agcaattacc ggataattac caggcaaaat
     1201 ttgagttaaa tttaatgagc aagttctcaa attttattat aaataaacca ttttcagcga
     1261 taaatactgc ggcacgtcac attttttcac gctatttgtt ggagaacaaa catttatttt
     1321 atcaatattt taaaatttcg aatacaggta ttgatcatct cgaacaattg attaacgtca
     1381 actttttctc ttctgacagg acgtcatttt gtgaatgcaa tcgttttcca taaattcttc
     1441 tcccctcata ggcgacgaat agcattttgt gttgaggatc acaaaacgaa taattgctgg
     1501 tcgccgcgat aaggtcagac aaagacaaca agggaaattt tcacagagct tttgatcgtc
     1561 gtaggccaca gaatgttgct tcgtttactg taacgccggg taaatgagcg ttttttgata
     1621 gtgcgaaaga accctgcgag gaaaataagc atctattatt gtttgtcatg acgacaaggc
     1681 aggcatcaat gaaaggcttc ccaattgcac atatttttca cccttcaatc ccgccaatgc
     1741 acgcagtggt taacaatcac aatagaaata ttgattattg gacggtaaaa agaaagtttg
     1801 cagaaattgt ctccaccaat gacgttaata aaatttacag tataagtaat gaactgcgga
     1861 gagtattatc tgcaattact gcattgaatt tctatcaagg cgatgttcct tctgtaatga
     1921 tccgaatcca accggaaaat atgagtccat tcatcataga tatttctaca ggggaacatg
     1981 atgattatat catacaaaca ttagatgtag gcacttttgc accttttggc gaacaatgta
     2041 cttgctcagc cgtcaataaa aaagagctgg aatgtattaa ggagacgatt tctaagtatt
     2101 gtgcaaaatt cacccgaaaa gaagccatct taacacccct tgtgcacttt aataaaacca
     2161 gcattacttc agattgttgg caaattctct tt
//
LOCUS       DAZUFB010000273         2175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZUFB010000273 DAZUFB010000000
VERSION     DAZUFB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.277"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHY3M_005279"
     CDS             <1..783
                     /locus_tag="ACHY3M_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFS4319971.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARQEQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            824..1246
                     /locus_tag="ACHY3M_005280"
     CDS             824..1246
                     /locus_tag="ACHY3M_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS4319972.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNTRKKLSRAKLKKPAVVDPDLIWSLPDG
                     EIRRYDRHQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            1243..1539
                     /locus_tag="ACHY3M_005281"
     CDS             1243..1539
                     /locus_tag="ACHY3M_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS4319973.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAERLGHAVIYQEVAQ"
     gene            1536..1997
                     /locus_tag="ACHY3M_005282"
     CDS             1536..1997
                     /locus_tag="ACHY3M_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFS4319974.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRR"
     gene            1975..>2175
                     /locus_tag="ACHY3M_005283"
     CDS             1975..>2175
                     /locus_tag="ACHY3M_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319975.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWM"
BASE COUNT          603 a          471 c          652 g          449 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc cggcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcga gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggcagga gcaatcacac
      361 caacttcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg gtcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggagtacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatgctgg atggcgtgag gcgttacgca
      601 agatacttgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttggaccgg accggaattt tgagaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataacgaaa ttccgccggg cttcaggggg
      781 taagtgttaa tttctggtca tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac
      841 acaagaagag cgggaaaaaa ttaaagggca ggttcttgaa ctcgtacgcc agagtgggcg
      901 cgagacgtta cgacaactgg aagctaaaac tggggcaaca agatatctga tgagcgttct
      961 ggccagagag ctggttgcca gtggcgatgt atacaactct ggttacgggt tattcccgtc
     1021 tgaacaggcg cgtaaggact ggcaaaatac ccgtaaaaag ctctcaaggg caaagctgaa
     1081 gaaacctgca gtggttgatc cggaccttat ctggtcgtta ccagacggcg aaatacgccg
     1141 ctacgacagg caccagaaca taatttgtcg tgagagcagg aggagcgaag ttatgcagcg
     1201 tgtgctggcg ttctatcggg gaaactttca ggaggtgatg gagtgagggt gagagtttat
     1261 attgccggtc caatgacggg atatgaaaat ttcaaccgtg aggcatttca caaggtggaa
     1321 gaggaactga aacgggaagg gcataccgtc ttaaacccgg cagtacttcc ggacgggctg
     1381 acacagccgc actacatgga tatttgcatg gcaatgattc gttgtgtgga tgcgatttac
     1441 atgctgaatg gctggcagcg gtcagcgggc gctaaggcag agctggcact ggcggagaga
     1501 ctggggcatg cggtgattta tcaggaggtg gctcaatgag agaggttaac tatgaggcgc
     1561 ttcgtgaggc agcacaaaac tatcagtcga cgctggcgtg gtatcaggct accccggaca
     1621 gcccaaatgc tgaacgggat tgtgatgcgg ctcttgctgc gtttaagcgt catatccgtc
     1681 atcgggaagc ggatattatc gctggtttgc tggatggact ggaagaagca aaatcacaac
     1741 tcaacgagca gcgtgagtat tacgaaggcg ttatctctga tgggagcaag cgtattgctg
     1801 aactggatgc gcgggaagtt caattaccga ctcgctacga ccttcgatat ggacacccga
     1861 taaatgcaga tgagcgacat gtcatgatac ctaaagaaaa tggcagttgg ctttacctga
     1921 ttgacctaga acacgcatta cgcgtcgctg gcattcgcat caaaggagag gagcatggaa
     1981 ataaaaccag aagatgagtt aagcaatatc gttttatttc cggtaaaaga ggatgaccct
     2041 cgtaatcagg ttaattttct ttatgagcca tcggaaagac catattgtca tcacgcctct
     2101 gtccgggttg acgaaaaaga gcgtcaggtc cgctgtaaaa tctgcggtgc agttgtggag
     2161 ccatttgact ggatg
//
LOCUS       DAZUFB010000274         2171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZUFB010000274 DAZUFB010000000
VERSION     DAZUFB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.278"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..469)
                     /locus_tag="ACHY3M_005284"
     CDS             complement(<1..469)
                     /locus_tag="ACHY3M_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4319976.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPT"
     gene            complement(469..810)
                     /locus_tag="ACHY3M_005285"
     CDS             complement(469..810)
                     /locus_tag="ACHY3M_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4319977.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(803..>2171)
                     /locus_tag="ACHY3M_005286"
     CDS             complement(803..>2171)
                     /locus_tag="ACHY3M_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4319978.1"
                     /translation="KQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDSYQE
                     ARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELA
                     KLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEA
                     ELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLS
                     ERLKDQAAFYAESDAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNIT
                     EMLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGA
                     VANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGV
                     RAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDG
                     GMLSG"
BASE COUNT          412 a          752 c          491 g          516 t
ORIGIN      
        1 cggtcggtgt agccagcaca aacgaggccg tcatggctgt cagctgcgac atctgctcca
       61 ccacccagcg gtcactcagc tcctgctccg ggtccgcttc cggattgccc gcaacgaaat
      121 tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac caccgtggcc cccaccagac
      181 tctgcaggtc ctccgccatc ccggtgacaa ggccaaacag attggacacc gtcagcgacg
      241 ggcgggcact gctgccccgg ccgttcatct caaagccgct gccgtcaatc gggtatgcct
      301 catacttacg cccctgccag gtgaccggct cccctttttc attcagctca ttacagaaaa
      361 aataccgctc accaccctgt accgtcaggt cgatttccca gagtaccacc cgcggtgact
      421 gctctgactt aaccgactcg ttcagacttt cttcgcgaat atcctgcatc agttcaccac
      481 ctgctcaatc gtacaactga aatcactgta cctggcgtta tctgtgacgc tccactcccg
      541 gcacaccacc ctcaccgtcc ggttatgttt cggcggtcgc cacaaaaagg cacggtaacc
      601 accatgccag gataaaaatt catccagcca gcgccgggtt ggttcatccg tcacccggaa
      661 caccgcctga aacgtcttca gtctggcatt aagtcccgtc ggtcggcgct gttcataacc
      721 gtcaccaaac cgaaccctca ccaccgacgg tttctcactc acctgcatcc cttcacgcgg
      781 gaccagatgc agcgttttta tctcagccac tcagcattcc tccgtcacgt cgcatggaca
      841 gcatcaccgc ctgcacccgc tggtcaatca gctgcacaag actgcctgcc gcctccggcc
      901 ctatctggcc attagtcccg tcattctgaa tggcgatatg gtagaccggg gaatacacca
      961 gacccgcact gccgttcata ctgcccaccg cgcgtacgcc cagcgagcca tccgccgccc
     1021 gggtcagggg cataatagct tcaggtccgg cttcccccat cagcccggcc ccttttgcaa
     1081 acgcaaagta cgtgggcgta tccacaatac tgttgctgta cgcactcagg tttgccgagg
     1141 tatacacgcc gccttttgca ttggccaccg ccccgcccag ccagtcacca atgctgccga
     1201 gaaatcctcc cgcaccggac ataccgtttg ccgccgtctt aattccgttg acaatcgcgg
     1261 cattcataag aacttttgag atttcctgca gtacggatga ggcccagttg cgccattcca
     1321 ctttgtttcc gttcagcatc tccgtgatgt tattcaccat ccctgagata ccctccgtcg
     1381 caagctgtgc tgcctgtgaa gcgtaatcgg atgcattgtc cacccagtta ctgagcccct
     1441 cctgcaagcc tttctgccag tccgcacgct gcgcatccga ttcggcataa aaggctgcct
     1501 ggtccttaag gcgttcgctc agatactgcg cgttctgtgc ccgtgcctgt ctgtaaaaat
     1561 cctcactgat atccccggtc tgatactgag actgaaggtc cgcatccttc tggcggaagc
     1621 tgtcgcggat ctgctgcaac tcccgcatgc gttccctggc tcgttctccc tgcccgtatc
     1681 ccagcagttc ggcttcattt gatgcacgcg cagccacatt atcattcttc agggtctctt
     1741 cccgggatcg caactgttcc cggatttttt gctggtcaat cagggccgcg ttacgcagca
     1801 gttcctgctt ctgcatctcc gtcagggttt tcagttcgcc ctgcgcagtc tggtacttca
     1861 gcttcgccag ctctgtattc tgacccgcca gtgccagttg ctctttctgc tgcttcagta
     1921 gccgggaaaa actgtcttcc gctttttccg tctctgattt tccaccccgg gatttaggtt
     1981 tgttcgcctc gttattacgc caggcttcca gagcattact gatataacgc tgtctcgcct
     2041 cctgatacga atcccccaca aaaccaaggt catccgccgc ataccccagc cggacacgct
     2101 ctttttcctc ccccttcagt cgggacaggg ccagctcacg ttctgttttt gtcagggcgc
     2161 tctgctgttt a
//
LOCUS       DAZUFB010000275         2165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZUFB010000275 DAZUFB010000000
VERSION     DAZUFB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.279"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..555
                     /locus_tag="ACHY3M_005287"
     CDS             <1..555
                     /locus_tag="ACHY3M_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4319979.1"
                     /translation="AGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRA
                     NSSLAMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPV
                     QETTTQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRVKGQVQKWERVC
                     AALRELNKCRDILRDITATREQQR"
     gene            588..869
                     /locus_tag="ACHY3M_005288"
     CDS             588..869
                     /locus_tag="ACHY3M_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4319980.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLRAVNNYVYFDRRVQA"
     gene            866..1162
                     /locus_tag="ACHY3M_005289"
     CDS             866..1162
                     /locus_tag="ACHY3M_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS4319981.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            1159..1620
                     /locus_tag="ACHY3M_005290"
     CDS             1159..1620
                     /locus_tag="ACHY3M_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4319982.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERQVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRR"
     gene            1598..1954
                     /locus_tag="ACHY3M_005291"
     CDS             1598..1954
                     /locus_tag="ACHY3M_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319983.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGSVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRASKSRTE"
BASE COUNT          626 a          460 c          631 g          448 t
ORIGIN      
        1 gcaggtgaaa gtgaaccagt gtcaccggta ccgaaagtga cagcgcaaat gctgactgag
       61 gcgattgaac aacatggccc acaaacggcg gatgagctgg cactgatgtt cgggattacc
      121 tcccgccggg cgaattcatc gctggccatg gcaatcagca aagggcgtct gattcgcgtg
      181 aatcagggcg gtaaatttcg ttactgcata ccgggcgctg atttaccggc agagccggaa
      241 gctgcatccg tagcggaaac cgatggtaaa gcctttcctc agccagcagg tgttgcgtta
      301 ccagtccagg aaacgacgac acaggaagaa atgaaaacag aaatcgtgga agacattgtg
      361 aagttacagc catcggtcac cgaaacgaaa gcagatgacc tgattctacc atcgctgcat
      421 gtggctaacc gcgagctgcg ccgggtaaaa ggtcaggttc agaagtggga gcgagtctgc
      481 gccgcgctgc gggagctgaa caagtgccgg gatattctcc gggatattac cgccaccaga
      541 gaacagcagc ggtgagtggg tggaagacgt ggtgccgggc tgaaattctg atactccggc
      601 agtgcgcggg aacaatgaag gtaaaaagca ttggcgcgct tatcggacga actgaagcgg
      661 cagtgagaac gaaggcacgg gagctgggca tcagcatgat gttacgtggt gattttcacc
      721 cgtcggcaaa atattcacag cgtgatattg agctggcgcg gcaactgcat cagcgcggtg
      781 taccccgacg ggaaattgcc gaaaagttcg gaatgaagtt gcgtgcagtg aataactacg
      841 tttatttcga caggagggtt caggcgtgag ggtgagaatt tatatcgccg gtccaatgac
      901 gggatatgaa aatttcaacc gcgaggcatt tcacagggcg gaagaagcac tgaaacggga
      961 agggcatacc gtcttaaacc cggcagtact tccggacggg ctgacacagc cgcactacat
     1021 ggatatttgc atggcaatga ttcgttgtgt ggatgcgatt tacatgctga atggctggca
     1081 gcggtcagcg ggcgctaagg cagagctggc actggcggag aaactggggc atgcggtgat
     1141 ttatcaggag gtggctcaat gagagaggtt aactatgagg cgcttcgtga ggcagcacaa
     1201 aactatcagt cgacgctggc gtggtatcag gctatcccgg acagcccaaa tgctgaacgg
     1261 gattgtgatg cggctcttgc tgcgtttaag cgtcacatcc gtcatcggga agcggatatt
     1321 atcgctgatt tgctggatgg actggaagaa gcaaaatcac aactcaacga gcaacgtgag
     1381 tattacgaag gtgttatctc ggatgggagt aagcgcattg ctgaactgga agcgcgggaa
     1441 gttcaattac cgactcgcta cgaccttcga tatggacacc cgataaatgc agatgagcga
     1501 caagtcatga tacctaaaga aaatggcagt tggctttacc tgattgacct agaacacgca
     1561 ttacgcgtcg ctggcattcg catcaaagga gaggagcatg gaaataaaac cagaagatga
     1621 gttaagtaat attgttttat ttccggtaaa agaggatgac ccacgtaatc aggttaattt
     1681 tctttatgag ccatcggaaa gaccatactg ccatcacgcc tctgtccggg ttgacgaaaa
     1741 agagcgtcag gtccgctgta aaatctgcgg ttcagttgtg gagccgtttg actggatgct
     1801 ctctgtggcg aaaagagaaa ccagactggc agatgatgta aagctattgc gccaggagga
     1861 acaggaaagg cgaaaaaata tagaaaagtt aattcagatt gagcgtaacg cgaaagcgcg
     1921 gatacgcagg gcgtcaaaat ccagaactga ataattaaat tcagcactgt taaaaattta
     1981 atccttaacc ggagggattc ctgcaccctc agaacataag gagaccgcct gaaagggtgg
     2041 taatgaaaaa tgactgaatt aaccaaagag caattaatca aggaagccaa attaaaaata
     2101 gcggttgcga actgttaccc taattcagag atggccaggg tagagggtga gctattcaaa
     2161 attgc
//
LOCUS       DAZUFB010000276         2152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZUFB010000276 DAZUFB010000000
VERSION     DAZUFB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.280"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..879
                     /locus_tag="ACHY3M_005292"
                     /pseudo
     CDS             1..879
                     /locus_tag="ACHY3M_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            962..1288
                     /locus_tag="ACHY3M_005293"
                     /pseudo
     CDS             962..1288
                     /locus_tag="ACHY3M_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1429..>2152)
                     /locus_tag="ACHY3M_005294"
     CDS             complement(1429..>2152)
                     /locus_tag="ACHY3M_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS4319984.1"
                     /translation="PQEHSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLFKIT
                     KSGDIMDRAPLPFISDAETIEHIQGNIFAAVDENTSELFFFTVTKDMHISFRNKIQLE
                     KFNKKNRGFEGLAWKADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEELNDIRI
                     NDISGLAFNNESLMILSDESRNLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVT
                     LSDESIYVASEPDILAKFVPNK"
BASE COUNT          608 a          432 c          521 g          591 t
ORIGIN      
        1 ttcgcccgtt catctgtcgg gcgctggatt aactggttca cacagtcggg tgttgatgga
       61 ctgaaatcat tacccgccgg gcgtgcccgc cgctggtcat ttgaacatat ctgtacactg
      121 ttacgtgagc tggtaaaaca ttctcctggc gactttggct accagcgttc acgctgaagc
      181 acagaactgt tggcaataaa aatcaatgag ataaccggat gtcagttaca tgccggaacc
      241 gtgcgccgct ggttgtcgtc tgcggggctg gtgtggcgca gagcagcgcc aactctgcgt
      301 atccgtgacc cgcataaaga tgaaaagatg gtggcaattc ataaagcact ggacgaatgc
      361 agcccagagc atccggtatt ttatgaagat gaagtggata tccaccctaa tcccaaaatc
      421 ggtgcgggct ggcagcttcg cggacagcaa aaacacgtgg tgacgccggg tcagaatgaa
      481 aaatattatc tggctggcgc gttacacagc ggaacgggaa aagtgagtta tgtgggtggc
      541 aacagcaaga gctccacttt atttatcagt ctgttgaaac acctgaaatc gacataccga
      601 cggtctaaaa ctatcatgct catcgtggac aactacatca tccacaaaag ccgggaaaca
      661 cagcgctggc tgaagcagaa tccgaagttc agggtgattt atcagctggt ttactctcca
      721 tgggtgaatc acgttgaacg gctatggcag gcgcttcacg acacaataac gcacaaccat
      781 cagtgccgtt caatgtggta gttgctgaaa aagattcacc attttatggg aaccgtcagc
      841 ccattccccg gaggtaatta tggacaggca aaagtgtagc ggtattaggc acagctattt
      901 agtattaaga ctatcactta tttaagtgat attggttgtc tggaaataag ggggcaagaa
      961 caagtggctg acggataatg gttcatgcta ccgggcgaat gaaacacgtc agttcgccag
     1021 gatgttggga cttgaaccga agaacacggc agtgcggagt ccggagagta gcggaatagc
     1081 agagagcttc gtgaaaacga taaagcgtga ttacataagt atcatgccca aaccagacgg
     1141 gttaacggca gcaaagaacc ttgcagaggc gttcgagcat tataacgaat ggcatccgca
     1201 tagtgcactg ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt
     1261 aagtgataac agatgtctgg aaatataggg gcaaatccac tggttgataa tagctgatgt
     1321 ccttttcagc tattaatgtg tttattttag cgtaaaatta tactcgtatt tttataactt
     1381 tatgagattg ccagatttgg tcgataaata ataatgccag tgacagactt acttattagg
     1441 gacaaatttc gcgaggatat caggttcact ggcaacataa atgctttcat cagacagagt
     1501 tacaattcct tcaggttggg gcaaatcact ggtcagagaa tgatttcctt ttgtcaggtc
     1561 aagcatctca atgaaagaca tctctgtcag gttaaacttg agtaaattac gcgattcatc
     1621 ggacagaatc atcagacttt cattattaaa ggccaggccg gatatatcgt taatacgtat
     1681 atcatttagt tcctctggaa tagttgcttg ctttacactc agcaggtctg gagaaagttg
     1741 atatataaat attttacttg ggcgtctttc ttttgcgaca aacagcatcc gatcatctgc
     1801 tttccatgcc agcccttcaa atcctctatt ctttttattg aatttttcta actgaatttt
     1861 atttcgaaag gagatatgca tatcttttgt aactgtaaaa aagaatagtt cagaagtgtt
     1921 ttcgtctact gcagcaaaaa tgttcccttg tatgtgttca attgtttcag catcagagat
     1981 aaaaggcagc ggggctctgt ccatgatgtc gccacttttg gttattttaa atagctgtgc
     2041 agggctattc tgggtggcat acaggaaatc atcctcttta ttataagtaa gagatgataa
     2101 gttcaccgta tttggtaatt gttttataaa tgaaagtgag tgttcttgag gt
//
LOCUS       DAZUFB010000277         2137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZUFB010000277 DAZUFB010000000
VERSION     DAZUFB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.281"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHY3M_005295"
     CDS             <1..224
                     /locus_tag="ACHY3M_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4319985.1"
                     /translation="DLSNWQFYSTNWEDVQEIAIPTLTIKEKINLDSKLPDKEAYQAA
                     AEEFSLNENERDNYYKYYRLYPNFQRIMV"
     gene            complement(192..1343)
                     /locus_tag="ACHY3M_005296"
     CDS             complement(192..1343)
                     /locus_tag="ACHY3M_005296"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4319986.1"
                     /translation="MNVIDLFSGVGGLSLGAARAGFDVKMAVEIDQHAINTHAINFPR
                     SLHVQEDVSLLNAEIIKGFFKNDMPIDGIIGGPPCQGFSSIGKGNPDDSRNQLYMHFY
                     RLVSELQPLFFLAENVPGIMQEKYSGIRNKAFNLVSGDYDILDPIKVKASDYGAPTIR
                     TRYFFIGVKKSLKLDISDEVFMPKMIDPVTVKDALYGLPDIIDANWQSDSESWRTIKK
                     DRKGGFYEKLWGQIPRNVGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGETDK
                     ISRSTRLDPNGFCPTLRAGTARDKGSFQAVRPIHPYHPRVITPREAARLQGFPDWFRF
                     HVTKWHSFRQIGNSVSPIVAEYILKGLYNLLNKRVQPEYLNHNSLEVRV"
     gene            complement(1937..2041)
                     /locus_tag="ACHY3M_005297"
                     /pseudo
     CDS             complement(1937..2041)
                     /locus_tag="ACHY3M_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          638 a          483 c          359 g          657 t
ORIGIN      
        1 gcgacctttc taactggcaa ttttattcaa caaattggga agacgtgcaa gaaatagcaa
       61 taccaacact aacaattaaa gaaaagataa atttagatag taaactgcct gataaagaag
      121 catatcaggc agccgctgaa gagttctctt taaacgaaaa tgaacgggat aactattata
      181 aatactatag gttataccct aacttccaaa gaattatggt ttaaatattc gggctgtact
      241 cttttattta ataaattgta cagccccttt aatatatatt cagcaactat tggtgacacg
      301 ctatttccta tttgtctgaa actatgccat ttagttacat gaaagcgaaa ccaatcaggg
      361 aagccttgta atctagcagc ttctcttggt gtaatcactc ttggatgata agggtggatc
      421 ggcctgacgg cttgaaagct tcctttatct ctagcggttc ccgcccttaa agtcgggcaa
      481 aaaccatttg gatctaatct tgtagatctt gaaattttat cagtttcccc aaaagataag
      541 gaagcataac gctcttggac gattttgcta tgtaatgttc cggtacatcc agatatgata
      601 ttatttttta acttagcgat cgattctgta tcaccaacat tccgagggat ctgcccccat
      661 aatttttcat aaaatccccc ttttcgatct tttttaattg ttcgccaact ctcagagtct
      721 gattgccaat tggcatcgat aatatctggt aatccataca aagcatcttt tacagtaacc
      781 ggatcaatca ttttaggcat aaatacttca tctgaaatat caagcttcaa tgattttttt
      841 acaccgataa aaaaatatct agttcgaata gttggagcac cataatcaga tgcttttacc
      901 ttgatgggat caagaatatc ataatcaccg ctaaccaaat taaatgcttt atttctaatg
      961 ccagaatatt tctcttgcat aatacctgga acattttctg ccaaaaagaa taatggctgt
     1021 aattctgata ctaaacggta gaaatgcatg taaagctgat tcctgctatc atcaggattc
     1081 cccttgccta ttgaactaaa tccttggcac ggaggaccgc caataatacc atctatgggc
     1141 atatcgtttt taaaaaaacc cttaattatt tctgcattta gtaaagaaac atcttcttgg
     1201 acgtgcaaac ttcttggaaa attaattgcg tgagtattaa tagcatgttg atcaatttca
     1261 actgccattt taacatcaaa tccagcacgt gcagcaccaa gacttagacc tccaacgcca
     1321 gaaaacaaat ctattacatt catatagttg caacgtctct tattagtttc tatgtattat
     1381 aacacaactg ttcttgatgt tttaacccca taagataacc tcataaaggc tatcccttga
     1441 aaataccgga tcgcataaat ttaatctttc aaaactactt gtctattaat cgagcaattt
     1501 aaggttgttt tagtcatgtc gataccgaca tagttaacta aacccatttc atcgtccaac
     1561 aaatgggggt aatacacttt gatttatagt cgggtgggtt tttctctatc tgcctttggt
     1621 gttcatgccc aaggcagata gcctcaagca cccgcagcaa ttctacttaa ctatcctttt
     1681 cccgcaaatc gtttttatcc ccagcggcaa atcgaataca caaccagcgc caccgccatc
     1741 gcaattccta ccgttgtgaa tgcttcaggc caggtcatcg tgaaacatcc tccacgctta
     1801 taagtccgct tcgctccagg tagtccatca ctttatccgg caatttgcag cccggtttcg
     1861 ctttcttcag ttgactaacc aattgtttaa ccagcattgt caattcgcga acttgtttcc
     1921 cgggctcccc tttgccctga agcagggcag cgcggcaagc gttccatccc tcagcatatg
     1981 tttcagttac accatcgaga tggcatgtaa gcaaatccat ttcttccggc actacgggcg
     2041 ctggaaaaac ggcatagggt ggcgtccatt ttggcgcttt atctccagcc gaacgctgct
     2101 accagtcatc cggtttgtat tcataaaaat caccaac
//
LOCUS       DAZUFB010000278         2112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZUFB010000278 DAZUFB010000000
VERSION     DAZUFB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.282"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1715
                     /locus_tag="ACHY3M_005298"
     CDS             3..1715
                     /locus_tag="ACHY3M_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFS4319987.1"
                     /translation="MLSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQRSVA
                     DKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTARYKQ
                     FDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPYDWD
                     IKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFLIDT
                     SGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAI
                     DSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKK
                     QRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITV
                     AKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLN
                     GQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSED
                     MRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVT
                     DISQ"
     gene            complement(1786..>2112)
                     /locus_tag="ACHY3M_005299"
     CDS             complement(1786..>2112)
                     /locus_tag="ACHY3M_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFS4319988.1"
                     /translation="PEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTL
                     LKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRA
                     SGKRVS"
BASE COUNT          607 a          461 c          514 g          530 t
ORIGIN      
        1 tgattttgtc aggatgcggg ccggaatctg agaataagga aagtcagcaa caacagccca
       61 gtacccccac agaccagcaa gtgcttgccg cgcaacaagc tgcaataaaa gaggccgagc
      121 aaagatccgt cgccgataaa gccactgcag acgcgaaagc aaaagcctta gcccagcaag
      181 aagcgcaaca atattcagac aaacaggctt tgcaggggcg cttacaggcg gctccaaaat
      241 atcaacatgc agccagagaa aaagcagcgt cccaaatcgc aaatccagga accgctcgct
      301 acaagcagtt cgatgataat ccggttaagc aggtagcgca aaacccgttg gcgacgttta
      361 gccttgacgt cgacaccggc agttatgcga atgtacgccg ttttctcaat catgggctgt
      421 tacctccgcc agacgctgtg cgggtggagg agatagtcaa ttatttcccg tatgactggg
      481 atatcaaaga caaacaatct attccggcca ctaagccaat acctttcgct atgcgctacg
      541 aactggcacc tgcgccgtgg aatgaacagc taacattgct gaaaattgat atcctggcga
      601 aagatcataa aagtgaagag ttaccagctt ctaatctggt ctttcttatc gatacttctg
      661 gttcaatgat ttctgatgaa cgtttgccac ttatccagtc ttcgttgaaa ttattggtca
      721 aagaacttcg tgagcaggat aacattgcca tcgtgaccta cgctggcgac tcccgtattg
      781 cgttgccttc tatctccggt agtcacaagg cggaaattaa tgccgcaatt gattcgctgg
      841 atgccgaagg cagtaccaat ggcggtgccg ggctggaact ggcttatcag caggcggcga
      901 aggggtttat taagggcggc atcaatcgca ttttattagc cactgacggt gactttaacg
      961 ttggcattga cgatccaaaa tcgattgaat caatggtcaa aaaacagcgg gagtctggtg
     1021 ttactctgtc gacgtttggc gtggggaata gcaattacaa cgaggcaatg atggtgcgaa
     1081 ttgccgatgt tggtaacggc aactacagct acattgatac cctctctgaa gcgcagaaag
     1141 tattgaatag tgaaatgcgg cagacgttga ttaccgtagc aaaagatgtc aaagcgcaaa
     1201 ttgagtttaa ccccgcgtgg gtaacggaat accgtcagat tggttatgaa aagcgccaac
     1261 ttcgggcgga agattttaat aacgacaacg ttgatgcggg ggatattggc gcaggcaaac
     1321 atataacgtt gttattcgaa ttaacgctga acgggcaaaa agcatcaatt gataagttac
     1381 gctatgcccc ggataacaaa tcagtgaaat cggacaaaac gaaagaactg gcctggttaa
     1441 agattcgctg gaaatacccg cagggaaaag aaagtcagtt agttgaattc ccgctggggc
     1501 caacaataaa cgccccctct gaagatatgc gtttttgcgc agcagtagct gcatatgggc
     1561 aaaagttacg cggttctgaa tacctgaaca atacctcctg gcagcagatc aaacagtggg
     1621 ctcagcaggc aaaaggggaa gatccacagg gttacagggc ggaatttatt cgcctgattg
     1681 aactggcgga tggtgtgact gacatcagtc agtgatgact gtttagcaaa ctatgttcga
     1741 ccagtcagca tatttgctga ctggtcgaat tgattaacaa tgatgttaac tcactctttt
     1801 gccggatgct ctatttttgg gttgtgagtg ttttatttct aattgtgtat aagaagcatt
     1861 gattcgtgca tcgccatatt tatctaaata acaatttctg tttaccagtc gatgttgaaa
     1921 atcaatttcg tactgctctt ctgctgaata ttttttaaat ctgtcagcat agttttttaa
     1981 taacgtatag ggggcgctgt cagaattgct ttccctttgt tcaatcactt cctgggctgc
     2041 catgcacaca aacgcaccgc aactttggct taagtattgc tgaagatcgg catgtaaaaa
     2101 tattatctct gg
//
LOCUS       DAZUFB010000279         2099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZUFB010000279 DAZUFB010000000
VERSION     DAZUFB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.283"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..230)
                     /locus_tag="ACHY3M_005300"
     CDS             complement(<1..230)
                     /locus_tag="ACHY3M_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFS4319989.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATAD"
     gene            complement(273..509)
                     /gene="ycgZ"
                     /locus_tag="ACHY3M_005301"
     CDS             complement(273..509)
                     /gene="ycgZ"
                     /locus_tag="ACHY3M_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFS4319990.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            823..2034
                     /gene="bluF"
                     /locus_tag="ACHY3M_005302"
     CDS             823..2034
                     /gene="bluF"
                     /locus_tag="ACHY3M_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFS4319991.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          613 a          435 c          416 g          635 t
ORIGIN      
        1 ttgtcagctg tcgctgttct tcggtttggc cctcctgaat cactattgct aaaatcacga
       61 attatgctgt caattaaatc gcgtcggaaa gcatcttcct cagccgattt cattacgagt
      121 aattgcttaa taagagtctg caatgaaaaa tttatcttca tgcgcagtat ctggatcacc
      181 gcctggccag taatttcata ttttatacgt tcattatcag atgtcttcat aacgacttca
      241 tagctaacct gttaaataat caggctgttg cattattcaa aaagcaaccc aattagtgca
      301 ttataatgtt tctgttcctc ttccccagtc gcgtgttcca gtcggcaaag aagtttggca
      361 caaagcgact ttcgactcag attacgtcca tctttcaaaa tctcagtcac aatttccccg
      421 agagtttcct gctgagtagg caagttagct tttgcaaagt aacgagtgat tgctcccgca
      481 gaatctaaag tcactgaatt ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg
      541 ttaagtgagg taacaatagc aaacttgtac gaaatatgtg tacagaatga taaattaatt
      601 ttttgcaatt agtgctaatg catataaaac aatgtgttat gataaaacat attgcatcat
      661 ttaactgaca atctatacaa gatttttcgc caactagtgt tcaacttttt ccatttttta
      721 tcaatgtaaa gaaacggtta tgttttggta ttaaaagaaa gacacaagaa gatatgtctg
      781 ttaccgtctt actctcgcct cacccattac cctggattgt taatgcttac cacccttatt
      841 tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata
      901 gcaaatcgca ggaacatgca gtctgacgta acagggatct tactgtttaa tggttctcat
      961 tttttccagc ttctggaagg tccggaagaa caggttaaaa tgatatatcg ggctatatgc
     1021 caggatccac ggcactataa tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt
     1081 tttggcaaag cgggaatgga attatttgat ttgcgcctgc acgagcgaga tgacgtttta
     1141 caggccgtat tcgacaaagg cacatcaaaa tttcagctaa cttatgatga cagagcgcta
     1201 caattttttc gtacttttgt ccttgcaacc gaacaatcaa cctatttcga gatccctgcc
     1261 gaagactcct ggctttttat cgctgacgga tctgataaag aacttgattc ctgtgccctt
     1321 tcaccaacta taaacgacca ctttgccttt catcctattg tcgatccctt atcgcggcgg
     1381 ataatcgctt ttgaagccat tgtgcaaaaa aatgaagata gcccatcagc catagcggtt
     1441 gggcagcgta aagacgggga aatctacaca gcggatctca aaagtaaggc gcttgcattc
     1501 acgatggcac acgcacttga gctcggtgat aaaatgattt caatcaatct attacctatg
     1561 accctggtta acgaacctga cgcagtctct tttttactta atgaaataaa ggccaatgct
     1621 ctggtgcctg aacaaatcat cgttgaattt actgaaagtg aagtcatatc tcggtttgat
     1681 gagtttgccg aagcgattaa atcgctaaag gctgctggta tcagtgtagc aattgatcat
     1741 tttggcgcag gttttgctgg tttgttactc ctgtcacgct tccagcctga cagaattaaa
     1801 atcagtcagg aattgattac caatgttcat aaaagtgggc cacggcaggc aattattcag
     1861 gcgatcataa aatgctgtac atcacttgaa attcaagtca gtgctatggg cgtggcaaca
     1921 ccagaagagt ggatgtggct tgaatccgca ggaattgaga tgtttcaggg agatctgttt
     1981 gctaaagcta aattgaatgg tatcccttcc atagcgtggc cagagaaaaa ataattttca
     2041 gcacattctt tcacatgatt tcagtaaatc atcgtgaaaa tgaatacgtt acgtttaaa
//
LOCUS       DAZUFB010000280         2084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZUFB010000280 DAZUFB010000000
VERSION     DAZUFB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.284"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..560)
                     /locus_tag="ACHY3M_005303"
                     /pseudo
     CDS             complement(40..560)
                     /locus_tag="ACHY3M_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            604..684
                     /locus_tag="ACHY3M_005304"
                     /pseudo
     CDS             604..684
                     /locus_tag="ACHY3M_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            843..1417
                     /locus_tag="ACHY3M_005305"
                     /pseudo
     CDS             843..1417
                     /locus_tag="ACHY3M_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            1618..1998
                     /locus_tag="ACHY3M_005306"
     CDS             1618..1998
                     /locus_tag="ACHY3M_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319992.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
BASE COUNT          604 a          380 c          471 g          629 t
ORIGIN      
        1 gattcaggaa aaccggttca gcatagccta ggctgaactt ttgaggatat caacgaacgg
       61 aatttttgaa catttatgag tagatcgttg ttgaagggct tcaatgagca tgtcaagttc
      121 atcaactgct ggtctacgat tacgacggtt tgatttacca atcaaagcaa gtttaagtag
      181 atatggacga gcacttttcg ccgggtttga tgtgtaatta attccgtata taggtttggc
      241 tgcatccaga acactgccaa gataactaac atcgtgactg actgttgctg gacctgcacc
      301 agcgttgttt ctcagcctgc aatgttcaat tacgtcattt tctgtcagtt cagatagttt
      361 gatcgcggag atgtcactat ccataagcag ttctagcaca tatcttttag tacggtctgc
      421 tttacctccg gcatttggtc atttaaatat ttgtgtagta agtcacggac tgtaagtccg
      481 tcaacagcat ttgatgatga aatgccatat agatctaata ccatcacttt ctgtgtgccc
      541 catgttttag catgagcatg aacgcggcgg ctatctcacg ggtgatcatc cctttggcat
      601 actgaaaagt atcgggtgtt actgaaccgt gttgatacat caactgcact ggatattgag
      661 tggccgataa taccgacgtt ataacctata aatgccaatc aggctgaatg ttgaaaggat
      721 agaatgaaca gcccgacaca caaaaaacgg agtccggctc cggtttttgt gttgccatat
      781 aaggcagatg tttgttacag ctatttaagt ctggagttca aattaaaata gggagttttg
      841 ttatgccatt aacctcagat attggatcac gttcatttaa tcttgggctg gaggtttttc
      901 gtgcccgaat tgcagccaat gggcgcggag atatacagta ggtggtgaaa ctgtcagtat
      961 tgtgtatgat gctactaatg ggcgcttttc atccagtggc ggtaatgacg gattgctttc
     1021 tgagttattg attttgggat ttaataatgg tcctcgagcc cttagtgaga gaatgctaag
     1081 tatgctttcg gactcaggtg aagcacaatc gcaagagagt attcaggaca aaatatctca
     1141 atgtaagttt cctgttagtt caggaaattt ccagtgcccg ccagagtcta ttcagtgtcc
     1201 aattacacta gagagacccg aagaaggagt gtttgtcaaa aattcagata gttcagcagt
     1261 atgcacttta tttgatgttg atgcactttc tcgcgtggtt aatgacggtt cagttcaccc
     1321 tctgacacga gctccaataa ccccatcaat gattgttaaa ccagaagagt gtaaatatga
     1381 ccctgcaagg ggaagcttta ttataaaaga tagttaagat gtttcaaatg aaacaatatt
     1441 aacttccgat aatttatata aaaacaccac aggcattcgg ggcctgtggt tggtaaaaca
     1501 atataatacg cgagttattt ttcatgagct ggagagaaaa caatcaaggt aagtagtatt
     1561 atttcacaaa ataccgggca ctttctggtg ccgcatcgtt cagagcttcg gtgtataatg
     1621 aatgaaatac gtagtaaccc tgtaaaattt ctggaagagc atttaattct taatacacag
     1681 cgctatgccc atcatgatga gacatcttta attacagtta atattacaag agaggatggc
     1741 ttgttaagac taaaagaaac ggaatatgat atggctggta gtgattatat tctgtttaca
     1801 gctatgcgtg atactgatag tccggagcgc tttgatgcac ctgcagttgt aagcttgagt
     1861 aatcagtgta taagcctccg gcgaaatgat atttctcaaa ctatccagca aagttcaccg
     1921 ttatggctaa ctaatcagca aagcggctgt agtgttctta ttgttcgcca tggattatca
     1981 gaatctggag aacagtagta ttcaatggta catatgtgcc caggggatag taacgatttc
     2041 gtcgatgagt ttacgctaat aatcaggaga ttttgttgga gatg
//
LOCUS       DAZUFB010000281         2078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZUFB010000281 DAZUFB010000000
VERSION     DAZUFB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.285"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..289
                     /locus_tag="ACHY3M_005307"
     CDS             <1..289
                     /locus_tag="ACHY3M_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFS4319993.1"
                     /translation="TVTVALEIGGYPSLLLPDVAISLDRTEGATLEFYEAEAKKQAKQ
                     FFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPGIITGSLKTPPLA"
     gene            340..873
                     /locus_tag="ACHY3M_005308"
     CDS             340..873
                     /locus_tag="ACHY3M_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4319994.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GTGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1144..1713
                     /locus_tag="ACHY3M_005309"
     CDS             1144..1713
                     /locus_tag="ACHY3M_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS4319995.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            1713..1859
                     /locus_tag="ACHY3M_005310"
     CDS             1713..1859
                     /locus_tag="ACHY3M_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4319996.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            1867..>2078
                     /locus_tag="ACHY3M_005311"
     CDS             1867..>2078
                     /locus_tag="ACHY3M_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFS4319997.1"
                     /translation="MNRVLCVVIIVLAVGCGALWLATNHYRDNALTYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSR"
BASE COUNT          551 a          444 c          579 g          504 t
ORIGIN      
        1 tactgttact gtggcactgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc
       61 gattagtctt gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa
      121 gcaggcgaag cagtttttca tggatgttgc tgccgggtta tgtgaagggg atggtccgtt
      181 gccggaaaag cggcccatca ttttagaggc gcaggatgtg ttgataacct acagaggaaa
      241 actaccggga ataattacgg gttctctgaa gactccaccg ctggcctgaa gacttaacat
      301 atccagggat ttgaaatcga taaatcctga taaatatcca tgaacgcaaa aatcagatac
      361 ggcctgtcgg ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc
      421 gaccagtttc tggatgaaaa ggaaggtaac cacaccacag cataccgtga tggtacgggt
      481 atctggacca tctgccgcgg tgccatcctg gtggatggta aacctgtcgt cccgggcatg
      541 aagttgtcga aggaaaaatg cgaccaggtt aacgccattg agcgtgataa ggcgctggca
      601 tgggtggaga aaaacatcaa agtgccgctg accgaacccc agaaagcggg gatcgcgtca
      661 ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt
      721 aatgcaggtg atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa ggacggtggc
      781 agggactgcc gtattcgctc aaataactgt tacggtcagg tatcccgtcg tgaccaggag
      841 agcgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgaggt
      901 ttgcttacat ggattgataa cacgaaatcc tgcaaattgg caaaatgtaa gtgaataaag
      961 tcaaaacagt tgtttaacac tcaggcaccg taatgatgcc tttgtcattt ctgcgcatct
     1021 cacgcgcatc tcacaacaca gaacctttca ggatgaccct tgaggatacc ggtttggctg
     1081 tcggtgcctt tctgtgggct ggattcctgt gagacaaggt tcatcactaa aaggaaataa
     1141 ccgatgaata tgatggccgt gccgtttcac ggcaactctc tttatgtagt taaccataat
     1201 ggcgaaccat acgttcccat gaaacctgtc gttgcgggga tggggctggc ctggcaatca
     1261 cagttggcta agttaagaca gcgttttgcg tcaactataa cggaaatcgt tatggttgct
     1321 gaggatggga aacaacgcaa tatggtgtcc atgccacttc gaaaacttgc cggctggcta
     1381 caaaccatta atcccaacaa agtaaaaccc gaaatccgcg ataaggtcat ccggtatcag
     1441 gaagagtgcg acgatgttct ttacgagtac tggacgaagg gttttgtcgt taatccccgt
     1501 aaaatgagcg tgatggaaga actcaaccag gcttgtgctg acatgaaacg ggataaaaac
     1561 attgccagtg tgtttgctac cgggctgaat gagtggaaac aggttaaagc cgcgcatgta
     1621 tcaaaaatcc gtacgctggt aaatgaagcg aatatgctga ttgattttgt cctggctgat
     1681 acaggcaaag ggaaaataac aaaggcggat tgatggggtg gctaatgata tcagataaac
     1741 tcataacgct ggtgaagagc ctctgtgtac ttgtcggcat ttcattttta gtcatgctgg
     1801 ttgccatttt cttttccacc gcctggcgag tcctgacgtt atcgggactg gtggggtgaa
     1861 agagagatga accgtgttct gtgtgtggtg attattgtcc tggcggttgg ctgtggtgcg
     1921 ctgtggctgg caacaaacca ttaccgtgac aacgcgctca cctacaaagc gcagcgcgat
     1981 aaaaaagcca gagagctgga acaggcgaat gccaccatta ctgacatgca ggtgcgccag
     2041 cgtgatgttg ctgcgctcga tgcaaaatac tcgaggga
//
LOCUS       DAZUFB010000282         2022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZUFB010000282 DAZUFB010000000
VERSION     DAZUFB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.290"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHY3M_005312"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHY3M_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4319998.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHY3M_005313"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHY3M_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4319999.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRRRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..>2022)
                     /locus_tag="ACHY3M_005314"
     CDS             complement(1944..>2022)
                     /locus_tag="ACHY3M_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4320000.1"
                     /translation="VYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          552 a          559 c          450 g          461 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccggcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga cc
//
LOCUS       DAZUFB010000283         1960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZUFB010000283 DAZUFB010000000
VERSION     DAZUFB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.291"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(295..918)
                     /locus_tag="ACHY3M_005315"
     CDS             complement(295..918)
                     /locus_tag="ACHY3M_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS4320001.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(915..1580)
                     /locus_tag="ACHY3M_005316"
     CDS             complement(915..1580)
                     /locus_tag="ACHY3M_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFS4320002.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(1577..>1960)
                     /locus_tag="ACHY3M_005317"
     CDS             complement(1577..>1960)
                     /locus_tag="ACHY3M_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFS4320003.1"
                     /translation="RSYWIKQAQQAVNAFIRERDRDLPCISCGTLTSAQWDAGHYRTT
                     AAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEAVEEIESNHNRYRWTV
                     EECRAIKAEYQQKLKKLRNSRSEVA"
BASE COUNT          461 a          496 c          426 g          577 t
ORIGIN      
        1 cgcaccatcc ttcataacca ccccaataca agatgataac attggcttac aactcataac
       61 aaaagcaatt caatgccgtc aagaggttac aggctaaaaa aactctatta cattgcagtc
      121 agcatgttta ctacacaaat acaattcaga gcataaaaac tactcggcgg caggttattg
      181 agactcatca atgacatgta aaaaacgccc attattggtg tcaagtttcc ccaaagttat
      241 tcaaaaagtc aatattatgc cgttaatatg ttgccatccg tggcaatcat ggcgctaacg
      301 tgtgatcgca ttcaaaatgt tgtctgcgat tgactcttcc ttgtggcatt gcacaaccag
      361 agcgtcatac agcggcttaa cagtgcgtga ccaggtgggt tgggtaaggt ttgggattag
      421 catcgtcaca gcgcgatatg cggcgcttgc tggcattctt gaataaccga cgcctttaca
      481 tcttccgcac tctttctcag caactatccc ccactgctct gttttggcta tatcaaccgc
      541 acggcctgta ccgtggcaat ctctgcatct tgcgcccggc gtagcggcac tacggcaata
      601 atccgcataa gcgaatgttg cgagcacttg cagtaccttt gccttagtat ttccttcgag
      661 ctttgccaca ccacggtatt tccccgatac cttgtgtgca aattgcatca gatagttgat
      721 agccttttgt ttgtcgttct ggctgagttc atgcttaccg cagaatgcag ccattccgaa
      781 tccggcttgt gattgcgcca tccccatagc agccatcaca tcagtaccag aaagagagtc
      841 agaagccgta gcccgtggtg agtcgctcat catcgggctt tttggcgaat gaaatttagc
      901 tacgctttcg agtctcatgc gccttctccc tgtacctgaa tcaatgtgag gtttccgcag
      961 aacactgcgc cagtatcgat atacatctgg ttggcaaatt tgagtggttt cactgctggc
     1021 gtatgaccaa agatgaacgt gtccgcgcct ttgatttctt tcacgatccc gtcttgtgag
     1081 ttgctgattc gttcgcggtt ccagattacc tgctgatgat caactggctt tccaaactcg
     1141 tatttatcac aaggataatc ggcgtggcag atgacatatt tttttccttt actcaccagt
     1201 tcgatgatta acggaagttc atctgcttta tgggcaagag ctttagccag aatttctttg
     1261 tcgtaatcga gattaaagaa ccagccaccg ccattaagca gccagtgatt gacgtttcca
     1321 cgctctgata agccatcaat catcatttgc tcatggtttc cacgtacagc tctgaaccag
     1381 gggaatgtga ttaattccag gcattctacg ttctctgtac cgcgatcaac caaatcgccc
     1441 accgagataa gcaggtcttt tttggtgtcg aatcctatcg tctccagttt tttcatcagg
     1501 ttcgtgtagc atccgtgcag atcgccaact acccaaatat ttcggtattt gctgccatca
     1561 attctttcgt agatattcat gcaacctcac ttctgctgtt tcgcagtttt ttaagtttct
     1621 gttgatactc cgccttgatg gccctgcact cttcgacagt ccagcgatag cggttatggt
     1681 ttgattcgat ttcctctact gcttcctgcc cgatgcggct aatcagttcg acgcgatacg
     1741 gaacgagatt tccgcttttg tgctggttgc acaccacgca ttgcttgtga atattgcgtt
     1801 catcaaatcg gagttgaggt gccgcagcag ttgtccggta atgtccggca tcccactgag
     1861 cagacgtgag cgttccgcac gagatacatg gtaagtcgcg gtctctttct ctgatgaagg
     1921 cgtttacggc ttgttgggct tgtttaatcc agtaactgcg
//
LOCUS       DAZUFB010000284         1953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZUFB010000284 DAZUFB010000000
VERSION     DAZUFB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.292"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..888)
                     /gene="yhaJ"
                     /locus_tag="ACHY3M_005318"
     CDS             complement(<1..888)
                     /gene="yhaJ"
                     /locus_tag="ACHY3M_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFS4320004.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFS"
     gene            993..1694
                     /locus_tag="ACHY3M_005319"
     CDS             993..1694
                     /locus_tag="ACHY3M_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFS4320005.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1717..1881
                     /gene="yhaL"
                     /locus_tag="ACHY3M_005320"
     CDS             1717..1881
                     /gene="yhaL"
                     /locus_tag="ACHY3M_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFS4320006.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
BASE COUNT          473 a          542 c          502 g          436 t
ORIGIN      
        1 gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
       61 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
      121 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
      181 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
      241 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
      301 tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
      361 ggcaacgtag acgttcatta acgtatagag cttgcgcgag ttgatctccg acgaggaacg
      421 aaaatgcata tccggcgcga taacaatatc cgcccgcccc tgctccagtc gttcccacgc
      481 ccccgccagc acttcggtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
      541 gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt
      601 ttcccaaccg cgcgcgaggg cttccgcatc ggtagtcagt ttatctgcgg cttccagcaa
      661 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatgacccga
      721 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
      781 ggaaggcacg cgtcccagct catccgccgc cgccgcaaaa ctgccccggc gatcgatcgc
      841 atccataacc cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt
      901 caggaaattt gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta
      961 ccataaaagg aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt
     1021 gtggacaagc agactacgga tggttgcagg cccggtatac tttttccttt ggacactact
     1081 tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct taaccaggaa gtgctggccc
     1141 caggtgccgc ctttcagccg cgaacctatc ccaaagtcga tattttaaat gtgattctgg
     1201 atggagaagc agagtatcgc gacagcgaag gcaatcatgt tcaggccagc gccggtgagg
     1261 cgttgctgct ctctacccag ccgggtgtca gctacagcga acacaatctc agcaaagaca
     1321 aaccgttaac acgaatgcag ctttggctgg acgcctgccc gcagcgagag aatccgctga
     1381 ttcaaaaact ggcgcttaat atgggcaagc agcaattaat cgcctcgcca gagggtacga
     1441 tgggaagcct gcaattacgc cagcaagtgt ggctgcacca tatcgtgctc gacaaaggcg
     1501 aaagtgcgaa tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat
     1561 ttcatgcgct tacgcatcat gaagagaaag cggcgctgac ctgcggtgat ggggcgttta
     1621 ttcgtgacga ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag
     1681 atttgcctgt ctagttgttt ttacagggag atgatgatga gtaaaaaatc ggccaaaaag
     1741 cgccagccag tgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa aaattttggc
     1801 tatgaagaga tgttgagcga gctggaagcc atcgtcgcgg atgctgaaac gcgtttagcc
     1861 gaggatgaag ctaccgcgta aagacaggtt gccggatgcg gcgaaaacgc cttatccggc
     1921 ctacaaaatc caacaacatt aacgaattaa aca
//
LOCUS       DAZUFB010000285         1951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZUFB010000285 DAZUFB010000000
VERSION     DAZUFB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.293"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..141
                     /locus_tag="ACHY3M_005321"
     CDS             <1..141
                     /locus_tag="ACHY3M_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320007.1"
                     /translation="VMCEGLFHGKKFCEACGTGIYCRATCTKYVYRYQPDLYQDCGGA
                     LT"
     gene            329..762
                     /locus_tag="ACHY3M_005322"
                     /pseudo
     CDS             329..762
                     /locus_tag="ACHY3M_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            844..1044
                     /locus_tag="ACHY3M_005323"
                     /pseudo
     CDS             844..1044
                     /locus_tag="ACHY3M_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1305..1781
                     /locus_tag="ACHY3M_005324"
     CDS             1305..1781
                     /locus_tag="ACHY3M_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320008.1"
                     /translation="MRRFVQQAVDHWPNLLAFHFTFHSTEGNIYGQQIHAFCISFYQQ
                     IHKRITARTHTTSPSSPLVLHWLLEQHGGAVIRCVLLLSQTSICHPRASVTVDEECSK
                     LVDLLQQIWPVISTGGQCQVERCFQVARGDTSEQYVALKTDALSFAVSDHKSSGAE"
BASE COUNT          474 a          462 c          501 g          514 t
ORIGIN      
        1 gtgatgtgcg aggggctttt tcatgggaag aagttctgtg aggcctgtgg tacggggatt
       61 tattgcagag caacatgtac gaagtatgtt tacaggtacc agccagattt gtaccaggac
      121 tgcggaggtg cactcactta atccgaaatg cccgctctgc tcagggcgtt caggctgact
      181 atcaatggca tacattttat ccggtgtttc gcacccacac cgtgcaacga ctcgacaccg
      241 gatatccgca gtcccgctta cctgaataaa acccggggga ttgattgtgt actgtggtgt
      301 gaaatcgcac cgtgatcgct gattcgccga atccgcactg tggtgtcaat caaccacacg
      361 cgagagtcgt ctcaggcttc gcctgcctca ggacgatttc tgcatagcta attcccgggg
      421 caacatttat agaggcgctg tgcgctctga tggtcgccac ttctgacccg gaccatgctc
      481 ctgatatgtc ggaatataat gtgatgttag gcatcagaga ggaatatttc gatgccccta
      541 atctggggcg tttgattctg gatgtctgga gcaatccggg ctttgtgtca ccggacgaat
      601 caaagcagta ccacccggga tgtggcgctg gatcgtcatt caccggactg tgaagacata
      661 cttaaagagc tgatatgggc gttcagtgat ttcgcccgcg atcatcgtgg tgtgtgtgat
      721 ccggaagccc gttacctcgc cggtaatcct tggtatccgt agcaggccag ctctgaaagc
      781 acagctcagc agattccccg cattcgcaca accttcataa atctcacaga ggacattgtg
      841 gccatgttga ccacaacaag ccacgacagc gtattgctgc gttccgatga tcccctgctc
      901 gacatgaact acatcaccag tttcaccggc atgaccgaca aatagtttta taaactgatc
      961 agtgaaggct attttcaaac tgggcggtac aaaagcgaag tggagcagtg gatgcagcag
     1021 cgaatagagg gagcgtgggg gtagcaggat gaaacgtgtt gtgatgccgg tacgctggta
     1081 ttgtgcaaaa tgccgacgct actattgtgg aaatcagccc tgtccctggt gctggcgata
     1141 ctccctgcag acattctccg gtcagccaat aggttgttgc tctttcctga tcgattcgtc
     1201 attccattct ttttgtttat aatgggcatt acactggtgt tggcgtgttt acctgcgtgt
     1261 ctgcaccggt tcagtaaaat tagaaaagtt cagagtcggg gaaaatccgg cgttttgttc
     1321 agcaggcggt ggatcactgg ccaaatctgt tggcatttca cttcacgttc cattcgacag
     1381 aaggaaacat ctacgggcaa cagattcatg cattctgcat ttccttttac caacagatac
     1441 acaaacgtat tactgcacgt actcacacga ccagtccatc atcgccgttg gtgttacact
     1501 ggctgctgga acaacatgga ggagcagtaa tacgatgcgt attgctcctc agccagacga
     1561 gtatttgtca tccgcgagcc agcgtcacgg ttgatgaaga atgttcgaaa ctggtggatt
     1621 tattgcaaca gatctggcca gtgataagca ctggcggaca atgccaagtg gaaaggtgtt
     1681 ttcaggttgc ccggggtgat acatcagagc aatacgttgc gttaaaaaca gatgcgttgt
     1741 cgtttgctgt gtcggatcac aaatcatccg gtgcagagta gcattctggc tgtattctgg
     1801 taaatcagcg tattatcgat atattatcgt actatcagaa accgtatcgt ctgttgatgg
     1861 ctagactggc ccccctgaat cttcagacaa ccaatatcac ttatttaagt gatagtctta
     1921 ctactagttt ttagactagt cattggagaa c
//
LOCUS       DAZUFB010000286         1949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZUFB010000286 DAZUFB010000000
VERSION     DAZUFB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.294"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..322
                     /gene="escP"
                     /locus_tag="ACHY3M_005325"
     CDS             <1..322
                     /gene="escP"
                     /locus_tag="ACHY3M_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HFS4320009.1"
                     /translation="FNHIKKRSPDTLFIRMASLWKRLFYSSGRRRRYFEEGEQSFSIL
                     CGRLRGIVLTIKCSNGIIYLSITVSPNNRNHVFLYHKKDYVFDKLKEIFPDETIEFTI
                     EYEN"
     gene            285..1202
                     /gene="escQ"
                     /locus_tag="ACHY3M_005326"
     CDS             285..1202
                     /gene="escQ"
                     /locus_tag="ACHY3M_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFS4320010.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSEGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGKNRLNVIILETTVQSLQYLINDNWQFVPHS
                     NTLFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWLVINNPLATVKLSDNNL
                     SITDVQAADPLCTISSETVMNRIYCAIGTINVDIHMLRNVQKDDIIDSTGYHLLGGCQ
                     LVRNNITIAYGSIIKINEDFYFTISVVCD"
     gene            1233..1742
                     /locus_tag="ACHY3M_005327"
     CDS             1233..1742
                     /locus_tag="ACHY3M_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HFS4320011.1"
                     /translation="MSSSLSGITFATSLTSNQASWSKLTRTLHISNDDTVDELQLKAR
                     MQQRHHRVFPDIDDYSVINFRGKEYAVRFIKENDNYVYKVHKITQESGCFSKIFSFLY
                     GGVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     FNSLSGVMG"
BASE COUNT          593 a          314 c          411 g          631 t
ORIGIN      
        1 ttttaatcat ataaaaaaaa gatcgcctga tacgttgttc atacgaatgg cttctttatg
       61 gaagagattg ttttactcct cgggccgacg acgacgctac tttgaagaag gggagcagtc
      121 gtttagtatt ctttgtggaa gattacgagg aattgtttta acaattaaat gtagtaacgg
      181 aatcatttat ctgtctatta cagtaagccc caataataga aatcatgttt ttttatatca
      241 taaaaaagac tatgttttcg ataagttaaa agaaatcttt cctgatgaaa ccattgagtt
      301 cacaattgaa tatgaaaatt aacgattttt atttgccatt gttgccagtc atcggaaccg
      361 gtcgcctcta tattacgtcc gaaggtcatg cgtgccatgc atattttcgt gaagtcagcg
      421 gtcatggtat tcgtttcacg ttgacatact cgggctatga ggggcgtttt tggatatcag
      481 aagaacagtt cattcagtgg tgccaggagt tattcccgta tagcgaaagc cggctgatac
      541 cggaagatac gataaaactc atgatcttat gggtaatgca gactgcctta ccggaaggtg
      601 atgtgtcagt tgatgatgtt caatttacga tgctaaataa agatgtttat ccggtaatag
      661 aaaataataa tggaaaaaac agacttaatg taattatact cgaaacaacg gtacaaagtt
      721 tacaatatct gattaatgat aattggcaat ttgttccaca ttctaatacg cttttttttg
      781 atggctatat cgtacctggg tggactgatt atccggttac agagttgaga gttggtgata
      841 gtctgagact ctatcatgtt gatgatagtc aagagagaag gtgttggctt gttattaata
      901 accctttggc aaccgtaaag ttgagcgata ataacttatc tattacggat gttcaggccg
      961 cagatccgtt atgtactatc agcagtgaga ctgtaatgaa tcgcatttat tgtgcgattg
     1021 gcacaataaa tgttgatata catatgttgc gtaatgtcca aaaagatgac attatcgatt
     1081 caacgggata tcatcttttg ggtgggtgcc agttagtacg gaataatatc acaatagcat
     1141 acggaagtat tataaaaatc aacgaagatt tttattttac tattagcgta gtatgtgatt
     1201 aatcaggagt gcgttatata gggaggtgta tgatgtcgtc atcattatca ggtataacat
     1261 tcgcgacttc tttgacaagc aatcaagcgt catggagtaa gttgacccga actcttcata
     1321 tatcgaatga tgatacagta gatgaactgc aacttaaagc acgcatgcag cagcgtcacc
     1381 accgggtttt tccagacatt gatgactatt cagttatcaa ttttcgggga aaagagtatg
     1441 ctgtccgctt cattaaagag aatgataatt atgtatacaa agtacataag ataactcagg
     1501 aaagtggatg tttctcaaag atatttagct ttttgtatgg aggcgttacg aaggccctgg
     1561 aaagtaaatt aaatgagaga cacattacgc cactttcttc aacctggttt cctcgcactg
     1621 cactggaagg ctttttaact gaaagagggc tttcttcttt gttacgtcgc gtacagtcta
     1681 cagaaggtgt gaaccctcca gagaatacta cattcaactc tttatcaggc gtgatgggtt
     1741 aagtattatt ttatgttatt ttcattttct ccccagaatg ttttctgttc tggggcgatg
     1801 tttcattatt tttttgaagt gtttcaagga tagagcactg cagttttttg tgaaatatga
     1861 actgaatgga tgctaccatc cagttcatat tataaatttc cgaagtaaag agcaaacaaa
     1921 tcaaagtgaa agtgatttta ttatttctt
//
LOCUS       DAZUFB010000287         1930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZUFB010000287 DAZUFB010000000
VERSION     DAZUFB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.295"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1767)
                     /gene="nleL"
                     /locus_tag="ACHY3M_005328"
     CDS             complement(<1..1767)
                     /gene="nleL"
                     /locus_tag="ACHY3M_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFS4320012.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLYKDGEVQNTFGYSLSNLFS"
     gene            complement(1787..1876)
                     /locus_tag="ACHY3M_005329"
     CDS             complement(1787..1876)
                     /locus_tag="ACHY3M_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320013.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
BASE COUNT          651 a          400 c          283 g          596 t
ORIGIN      
        1 agaaaaaaga ttgctcaatg aatacccaaa tgtattttga acttctccat ccttatataa
       61 aaaaaaggac atccaattag ttgatgtatt aggagataac atatcaacca tatgactcaa
      121 agaacacatc attgccatac catcatcctg tgatgataac aaaacccagt tataagccaa
      181 atcggaatca tcatcccagt caggcttccc agagccatcg cacaatccaa aatcactctg
      241 ctgtgtgaca ggataaactt tttcattttt taaatatact tcatagagtt ccctaaatcg
      301 ttcctgcatt ccatcacgcc gtgaaccatt cattacaatt tgtataaagg aactattata
      361 agataccatc gcacctggat gtcgctcaaa atacatccct gcctgcaata aactaccatt
      421 ctccatatac atcattattg aattattaaa ccaagacatt atagtatcaa aaaagtcatc
      481 acatataggc tcgagccatt gctgtatatt tgcgttgttc aaataaggat gaatactaaa
      541 tgaatcagcc aaagacatct taacataggg ccggaacgcc gctaattcgt caagagatat
      601 ttttttgata agttcttcag cggcccttac tttatcatgg ttatacttat catcagatgt
      661 attcatagtc aataaaattg atttatttga ttttgtatag ttatccaaca cagataatat
      721 attttcctgt atgtcagata gatctatgta acaccctcct agattaatgg tattactttt
      781 ttcactaaag agctccttta catcacctaa gatagcacca cttaaaacca ttccaggaat
      841 tataatagct cctgttaaat cagcctcgtt caaaattgga ggatattgta tatatgaaga
      901 tcctttaaat gatgcatttt taagattgga attgctgaaa ttagagtttt caaacaaagc
      961 cctaataata caattagata aattagcata acaaaaatta cattcattca aaatcgagtt
     1021 tttaaaagaa cagttttcca atacagcctg agacaaatca ctcatcctga gatctgtatc
     1081 atcaagcact gctccagaaa aatttactga tgataaattc agccctacaa gacttagacc
     1141 tgacaagtca catccagagt aattcaattc ttcagcagac tcttcacccg ttcggttagc
     1201 tgacaaccaa attaagtcag cagtaagctc agctttgctc aggcaggcac ggccttgact
     1261 gacatcatat gcaaggaatt gacattgcga ttcatttaat gaattacagc catttaaata
     1321 tacggaattt ctgagtgcct caggaaagga accttctata tgtttcaaag aactacaatg
     1381 gcataaagat aaattaatga cattttcagg tatgctgcaa tttataacct ctaatgatga
     1441 gcatccaacc atacttaatg aagataagtt ggggggtaag cagtttattg atttaagctc
     1501 tgtacatcca ttcaaaacca gttctttcag agaacctgga agcaagtctg gtaatgttgt
     1561 tattggctca ctgattgata aagtctctcc attagtactt ataacatcaa gtatttttga
     1621 tgcaacctca tgacgatttt cgctaaattc tccctcagcg caccactttt cgagggcaac
     1681 ttcaacatcc tcgttagatg gtgaatctac aggactttca aaagatatta ctccagaatt
     1741 tacagagata tttgtagtgg gcagcattta tctcactcct ttttacttag tccactaaag
     1801 taaaattaga aaacacaact gccgggacaa ctttctgtcc ctgtacccac aaataagatg
     1861 tcagatttat cggcattaat ttaactccct attgaattat ctcctctttt ttatctaaca
     1921 aatattcccc
//
LOCUS       DAZUFB010000288         1929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZUFB010000288 DAZUFB010000000
VERSION     DAZUFB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.296"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /gene="yfdP"
                     /locus_tag="ACHY3M_005330"
     CDS             <1..183
                     /gene="yfdP"
                     /locus_tag="ACHY3M_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFS4320014.1"
                     /translation="IKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQGQL
                     LTLAGEPDGKLRAAGH"
     gene            249..1073
                     /locus_tag="ACHY3M_005331"
     CDS             249..1073
                     /locus_tag="ACHY3M_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFS4320015.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLTEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            1201..1737
                     /gene="yfdR"
                     /locus_tag="ACHY3M_005332"
     CDS             1201..1737
                     /gene="yfdR"
                     /locus_tag="ACHY3M_005332"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFS4320016.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            1728..>1929
                     /locus_tag="ACHY3M_005333"
     CDS             1728..>1929
                     /locus_tag="ACHY3M_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFS4320017.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPL"
BASE COUNT          527 a          436 c          443 g          523 t
ORIGIN      
        1 atcaaacaca aactctcata tgttcgcccg actaaccgcg ggaaaatttc cgaagaagac
       61 accaccgaaa cgccgatgta tgtcaatcgc ggtggtcgcc tgactattct gcaggaagac
      121 cagggacaat tactgactct tgccggtgaa cctgacggaa aactccgcgc agcaggtcat
      181 taatatcgtt cttaattaac tgattattta tctcatcact gaatatcttt atatagtgag
      241 gacttattat gtctcagaac ttagacgcaa ccgcaattaa tcaaatccat gcccttattt
      301 ctgctcaggg tgttaatgaa attatcagta agattggtgc cgatgctgtg gcattgcctg
      361 agaatttccg cattcatgat ctggaaaaat ttaatttaaa tcgcttccgt ttccgtggtg
      421 cgctttccac tgccagcatc gatgacttta cccgttattc taaagatctt gcagatgaag
      481 gcacccgctg ctttattgat gctgataata tgcgtgccgt cagtgtgctt aacctgggta
      541 ctattgatga accaggtcac gcagataaca ccgccactct caaactgaaa aagacagcac
      601 cgttctctgc tctgttgtct gttaatggcg agcgtaactc ccagaaatca ctgacagaat
      661 ggattgaaga ctgggccgac taccttgtgg gctttgatgc taatggtgac accattcagg
      721 caacaaaagc ggctgcggcg gtccgtaaaa tcacgattga agcaaaccag accgctgatt
      781 ttgaagataa tgacttcagc ggcaaacgct ccctgatgga gtctgtcgaa gcgaagacca
      841 aagacattat gccagtggca tttgaattta aatgcattcc gtttgaaggt ctgaaagaac
      901 gtccgtttaa attacgcctc agcattatca ctggcgatcg tccggtactg gttctgcgca
      961 ttattcagct ggaggcggtg caggaagaaa tggctaacga atttcgtgat ctgcttgttg
     1021 agaaattcaa ggacagcaaa gtagaaacct ttattggtac tttcaccgcc tgatttcatt
     1081 actgcaaatg cccctgaggg ggcatttatg gaaacgtaat ttactcaata atcgccggat
     1141 ggtgagggat tctttttacc agaattcagc gcggtgcagc gcatatacgt ggagaacaaa
     1201 atgtcattta ttaaaacttt ttccgggaag catttttatt atgacaggat aaataaagac
     1261 gacatcgata ttaacgatat cgcggtttcc ctttcaaata tctgtcgctt tgccggtcat
     1321 ctttcgcact tctacagcgt cgcccaacat gcggttcttt gcagccagct ggtaccgcag
     1381 gaatttgctt ttgaagcatt aatgcatgat gcaacagaag cgtattgcca ggacatcccc
     1441 gctccactga aacgccttct tcctgactat aaacggatgg aagaaaaaat tgacgccgta
     1501 atccgtgaga aatacgggtt acccccagtt atgagtacgc ccgtgaaata tgccgatctt
     1561 atcatgctgg caaccgaacg ccgcgatctc gggcttgatg atggctcttt ctggcctgta
     1621 ctggaaggca tcccggcaac agagatgttc aacgtgattc cactggcacc tagccatgcc
     1681 tacgggatgt ttatggaacg ttttaacgag ttatcggagt tacacaaatg cgcatgaatg
     1741 ttttcgaaat ggaagggttt cttcgcggga aatgtgtacc gcgagatctg aaagtgaatg
     1801 aaacaaatgc tgagtatctg gtgcgtaaat tcgatgaagt acgtgctgag gctcgcaacg
     1861 agggtattaa ctataccgca agccgtcttg ctgctgcttt caatcacgga tttatcaata
     1921 agcctttgg
//
LOCUS       DAZUFB010000289         1897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZUFB010000289 DAZUFB010000000
VERSION     DAZUFB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.297"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..455)
                     /locus_tag="ACHY3M_005334"
     CDS             complement(<1..455)
                     /locus_tag="ACHY3M_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS4320018.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSA"
     gene            complement(607..1206)
                     /locus_tag="ACHY3M_005335"
     CDS             complement(607..1206)
                     /locus_tag="ACHY3M_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4320019.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1274..>1897)
                     /locus_tag="ACHY3M_005336"
     CDS             complement(1274..>1897)
                     /locus_tag="ACHY3M_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320020.1"
                     /translation="MNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTVKNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          444 a          557 c          436 g          460 t
ORIGIN      
        1 gctgaagtgt cagcatttgc agcgctctct tctgcctgac tggctgatat gccggcattc
       61 ctcgctgacg tctccgcctc tccggcattc ttcttcgcct cctcagcgtg acgcgccgct
      121 tcttccacca tcagttcaaa acgacgcagt gcctccggcc ggacgtcatc ctccgacatg
      181 gcaccgagaa aatcattcag cgtccccggt tgagaatctt catacacggt gatggtcccg
      241 gcatgtgacg gaggaaatcc ctccaccaac agaatgacgc tgtactgacc gtactcaaca
      301 tccatgctgt aacgtccggc ttcatccgga ttttcagagg ccaccgtgtt caccaccacc
      361 gtgctgctgg ttcgtctggc cttcagcaca atggtgcagt tctgtactgg ttttcctgtg
      421 ccatctttaa gcacgccaga aatttttact gtcatacttt tccaccaata aaaaaagccc
      481 gcagcagtga cgccacgggc ttcaggacag tgtaacttta cgtttcctca aacgcagttc
      541 accccataag gtggatgaac ctgcgtatca taacaatatt tacagaagat aaatcggcgt
      601 ctgttgtcag aaacggtatc cgataccaac aataaatgca tccgatcgcc agtcgccact
      661 accggaacct tcataagcaa ggtcaatggt cacggattcg gtcgggttaa actgcacgcc
      721 agccccccac gccagagacg tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc
      781 gtgcgttttc cccttgttgt cagttacgcg gagataatcc ccggagaaag tcgacacacg
      841 gctgtaagcc acacccgcca tcgcatacgc gctgaaccat tcattcacgc gtacagacgg
      901 ccccgccatc acgctgaacc agcggttacg cacggaatct tcatgccagc gggtatcgct
      961 gtagcgcgtt ttttgctcat cctcagcatt ggcataactg aaggacgtaa tcagccccag
     1021 cgcgtccata aactcataac ggtatttcac gttaatcccg ttcagatcat cactaccggg
     1081 aacgttcgtc gaggcatgga gataccccgc gctcagcgtg gactgatgtt ctgctgcact
     1141 cgctggcgta gcagcggcga cctgccagac tactgcggac aaaataacag cacataattt
     1201 acgcataatt acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtatc
     1261 tgggttataa aattcagcta atcgtgatgc ctgcagtggc tttcttcatc accacaacca
     1321 gcaaatcgct gatacttgct gtgggatacc agttatttac cagccatgct gacaccgaaa
     1381 actccagtgt catgtgaccg tgaccggcag gcatatcaat aacaccactg taaatcagcg
     1441 tattatccag cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc
     1501 atgaggagta aatatcagta tgctctttct catgtttagc gccgctgaat gccaccgccg
     1561 gaataacaat ctgccggtca aacggctgat cgtcataaac cctgacggta atggttcctg
     1621 atggccaccg ctccggtgca cgggagtccc gggggaaagc tttgcccact gttttaacga
     1681 gatcgccttc aatctggttc gcggacaatt ttcccagaac ccgacagttc tcgttaatcg
     1741 tgacgttgtt gagcgtcccg gagttcgcat tcacgttacc gctgatatcg gcatttttca
     1801 ccgtcagccg cccgtccggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg
     1861 tgggagccgt cagatatttc aggaacactt cattcat
//
LOCUS       DAZUFB010000290         1871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZUFB010000290 DAZUFB010000000
VERSION     DAZUFB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.298"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..322
                     /locus_tag="ACHY3M_005337"
     CDS             <1..322
                     /locus_tag="ACHY3M_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFS4320021.1"
                     /translation="TDKEMPPEKQEKTEQTALAARLPEAKSSPRDELDDMGDAVPARR
                     LQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLMTLLSDAMFAAEMKGMLD
                     GTGD"
     gene            306..1637
                     /locus_tag="ACHY3M_005338"
     CDS             306..1637
                     /locus_tag="ACHY3M_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFS4320022.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALLRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(1639..1718)
                     /locus_tag="ACHY3M_005339"
     tRNA            complement(1639..1718)
                     /locus_tag="ACHY3M_005339"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1759..>1871
                     /locus_tag="ACHY3M_005340"
     CDS             1759..>1871
                     /locus_tag="ACHY3M_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS4320023.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAS"
BASE COUNT          449 a          486 c          574 g          362 t
ORIGIN      
        1 gacagacaaa gagatgccac cggaaaaaca ggagaagaca gagcagacag cgctggctgc
       61 cagactgccg gaagcgaaaa gcagcccccg agatgaactg gacgacatgg gggatgcggt
      121 gcctgcccgc cggttacagg aggccatcga cccggtactg gagccggtca ttgatgccat
      181 cagaacgcgg gggctggcgg aggcactggc agacctgcct gccctttacc gtgaaatgga
      241 tgattcccgc ctgatgacgc tgctcagtga tgccatgttt gctgcggaaa tgaaggggat
      301 gctggatggc acaggggatt gatttaggtt atgccgccac gcttccctca aaagaggcgg
      361 tggcatactt ccgcgccaaa ggggcgcata tcagctggaa ctggttcgaa acagacgcgg
      421 atgttcatgc ccgttcgttc acggcggcaa aagtggcacg cctggacgta ctgaccacac
      481 tacaggcgga agtgcaacgg gccattgatg aggggatttc acagaaagca tttatccgca
      541 cactgacccc ccgcctgcaa aagctgggat ggtggggaaa gcagattgtg gtggacagcg
      601 ccggtaacgc agaagaagtg cagctgggca gtccccgccg tctggcgctg atttacaacg
      661 tgaacacccg cgtggcttac aatgccgggc gttacacgca gatgatgaac aacacggaca
      721 cgcatccgtt ctggcagtat gtggcggtga tggacagccg cacccgcccg gcgcattccg
      781 cccttaacgg tctggtattc cgctatgacg atccgttctg gaatacacac tacccaccca
      841 atggctggaa ctgccgctgc cgtgtccggc cattgtctca gacccgtctg gatgcaatgg
      901 ggttatccgt ttcatccggt caggatcatc tttccacccg caatgttgag gctggcgtgg
      961 ataagcagac cggagaagtc agagaaatgc cggtgaccac atattcagat ggcaccagaa
     1021 ccatgacacc ggatgtgggc tggtcatata accccggttc ggcggcgttc ggcacagacc
     1081 aggcgctgct ccgcaagttg atcgaggtga aaagcccggc gttacgggaa atggtggttc
     1141 aggagatgaa caacagcccg gagcggcagc tggcgttccg catctgggca aaaaacatca
     1201 tgaaaacccg gcgaggcggt aacgatatcc gcacgctggg ctttatgacc gaaagcattg
     1261 cgcaggcggt ggaaagccgg acgggaacgc caccggcccg cctgctggcg atgagcggta
     1321 aaaatgtgct ccatgcggac agcatgaaac atcagaatga cggtatcgcc ctgacaccgg
     1381 aggatttcgg gcgcttaccg gcaatgctgg caaggccaaa agcggtactg tgggacaaac
     1441 ggcacaacaa tctgatgtat atcgtggaaa atcaggatgg cagtgtgcag attgccgtta
     1501 atgcaccgta cagcctgaag cgtcagccgg ataaactgga tgtgattgtt aatgcttacc
     1561 gggttatcaa tatggataag ctgaaatcgg atatccgggg cggaatgctt gaggtactgg
     1621 aaggagatat tgattaacgg tcagtggcgg gggtcgaacc cgcatacatg atccacccgg
     1681 aggcaggcac cgactttacc agttaagcgt acactgaccg cctgcttatt ttatattcat
     1741 attttatacg gaggcaatat gtcatccatt gatgccgccg tcgttgttga cgttacgcgt
     1801 ctccagcggg tctttgcccg gcttcagttt gtgggtggcg gaaaagacct ggcccgcagt
     1861 gtggcgtcca g
//
LOCUS       DAZUFB010000291         1801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZUFB010000291 DAZUFB010000000
VERSION     DAZUFB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.299"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..>1801
                     /gene="tir"
                     /locus_tag="ACHY3M_005341"
     CDS             150..>1801
                     /gene="tir"
                     /locus_tag="ACHY3M_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFS4320024.1"
                     /translation="MPIGNLGNNVNSNNLIPPAPPLPSQTDGASRGGAGQLINSTGAL
                     GSRLLFSPLRNSIADSVDSRDIPGLPVHPSRLATATSEICLLGGFEVLHDKGPLDTLN
                     KQIGASAFRIEQQSDGSYAAIGEKNGVEVSVILNSQELQSLQAIDIEDKGRFVFTGGR
                     GGGGHSMVTPASDIAEARAKILAKLDPNNHGGSQARNVDTRSVGVGSASGMDDSVVSE
                     TRTSSTASSVRSDPKFWVSIGAIAAGLAGLAATGITQALALTPEPDDPTTTDPEQAAS
                     AAESATRDQLTQEAFKNPENQKVSIDEIGNSIPSGELKDDVVAKIEEQAKEAGEAARQ
                     QAVESNAQAQQRYDTQYARRQEELELSSGIGYSLSSALIVGGGIGAGVTTALHRRNQP
                     AEQTTTTTTHTVVQQQTGGNTPAQGGTDAIRAEDTSLNRRDSQRSTASTHWSDTSSAV
                     VNPYAEVGEARNSSPARQAEEHIYDEVAADPNYSVIQNFSGNNQVTGRLMGTPGQGIQ
                     STYAILTNNSAGLRLGMGGLTGSGGSAVNTANAAPTPGPGRFV"
BASE COUNT          513 a          365 c          473 g          450 t
ORIGIN      
        1 aaatacagac atgcatttgg ggggcattat tttgcttgca tcaaaaatta gactgtgatt
       61 tatttggttt atgctcagct gttttatcgg ccgcataccg ttacgtcgat aaaggataat
      121 gtcaaatttc taaataaaag gatatatgta tgcctattgg taaccttggt aataatgtaa
      181 atagcaataa tttaattccg cctgcgccgc cactaccttc acaaacagac ggcgcgtcac
      241 ggggaggagc gggtcaacta attaactcta caggagcatt aggatctcgt ttattgtttt
      301 ctcccctgag aaattctata gctgattctg tcgattccag agatattcca ggacttcctg
      361 tacacccatc gaggcttgct actgctacat cagagatatg cttgcttgga ggatttgaag
      421 ttctccatga taagggacca cttgatactc tcaataagca aattggagcc tctgcatttc
      481 gtattgaaca gcagtcagat ggttcttatg ccgctattgg agaaaaaaat ggtgtagagg
      541 ttagcgttat attaaattct caagaattgc aaagcttgca agctatcgat attgaggata
      601 aaggccgatt tgtttttacc gggggacgtg gtggtggtgg gcattccatg gtcactcctg
      661 catcagatat cgcagaagct cgtgcgaaaa tactggccaa attagaccca aacaatcatg
      721 ggggaagtca agccaggaac gttgatacgc gttctgttgg tgttggaagt gcttcgggaa
      781 tggatgatag cgttgttagc gaaactcgta cttcatcaac agcttccagc gttcgttcag
      841 atcctaaatt ctgggtttct atcggcgcaa ttgctgctgg tttagcgggg ctggcggcta
      901 ctggtattac acaggcgttg gctttgacac cggaaccgga tgatcctaca accaccgatc
      961 ctgagcaggc tgcaagtgct gcagaaagtg cgacaagaga tcagttaacg caagaagcat
     1021 tcaagaatcc tgagaaccag aaagttagca ttgatgagat cggaaattct attccgtctg
     1081 gggaattaaa agatgatgtt gttgctaaaa tagaagaaca agctaaagag gcgggtgagg
     1141 cggccagaca gcaggctgtt gaaagcaatg cacaggcgca gcagcgatat gatactcagt
     1201 atgccagacg tcaggaggaa ttagagcttt catcgggtat tggttacagc ctcagcagtg
     1261 cattgattgt tggtggggga attggtgctg gtgtaacgac tgcgcttcat agacgaaatc
     1321 agccggcaga acaaacaacg acaacaacaa cacatacggt agtgcagcag cagaccggag
     1381 ggaatacccc agcacaaggt ggcactgatg ccataagagc ggaagacaca tctctgaata
     1441 gacgtgattc gcagaggagt acggcatcga cacactggtc agatacttct agcgcagtgg
     1501 ttaatccata tgctgaagtt ggggaggctc ggaatagttc accggctcgt caggcagaag
     1561 agcatattta cgatgaggtc gctgcagatc ctaattatag cgtcattcaa aatttctcag
     1621 ggaataacca agttaccgga aggttaatgg gaactccagg gcaaggtatc caaagtactt
     1681 atgcgattct gacaaacaac agcgctggat tgcgtttagg tatgggtgga ttaacgggga
     1741 gtggcgggag cgcagtaaat actgcaaatg ccgcaccaac gccgggacca ggacgtttcg
     1801 t
//
LOCUS       DAZUFB010000292         1795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZUFB010000292 DAZUFB010000000
VERSION     DAZUFB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.300"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(183..293)
                     /locus_tag="ACHY3M_005342"
     CDS             complement(183..293)
                     /locus_tag="ACHY3M_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320025.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            complement(346..750)
                     /locus_tag="ACHY3M_005343"
     CDS             complement(346..750)
                     /locus_tag="ACHY3M_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFS4320026.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(971..1702)
                     /gene="bluR"
                     /locus_tag="ACHY3M_005344"
     CDS             complement(971..1702)
                     /gene="bluR"
                     /locus_tag="ACHY3M_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFS4320027.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          502 a          376 c          374 g          543 t
ORIGIN      
        1 taaggtttca atttttgagt tatataggaa aggtttaacc tgttcctggc taaaatacat
       61 ataactggat gatgactaaa ccaaaacaca tgtgcgttaa gtattgaaac gggcgtatgg
      121 cacacccgtt gtttttataa atatattaac cgttataaaa taacgtatag aaagtcaagt
      181 gatcacattt caaatatcaa tttgatagta ttggcatggt gattatttat gggtagcaat
      241 aaaaggacag tatttatcat ccatagggat agtctctgta cttttattcc cattatgcta
      301 atgccttact gaattatgaa gcatttctta agcatccaac tttagctaga ttaatggttt
      361 attattttct acatcttcaa tatataaaag cgtattatca atggcgtagt aactgcgttt
      421 gttatgatta acatcagtaa cccaccggaa aacgcccgcg cctgctagtg ttgaacagta
      481 ttcccgaaat gtagattttc cgcaaatatg aagcaatgcg gcctctttta ttttagcagg
      541 gttcttggtc gtactaactt ttaacaggtt cctggttcct cttaataaca aaaccgtgtc
      601 atcgtgagta ataattctga tgttatccgt agccagataa taaatgtaat gtgcaatacg
      661 gtgatgtttt aattctgaat aaaaccagga gaagttttgc tcttttctca cttgctcaaa
      721 catcttttga aaaacaacga cctgatccat caggataata acctcttgtt agttgtgaga
      781 ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg tctgtccttt ggctgtgata
      841 tgtacagagt gtgatagagg gaatatctga attctcccgg tgagcatttt gcaacggacc
      901 agctccggta caaacgctgt tgtgggttca gattataaca ttctgtctaa ggggcgggat
      961 aaaggtgaaa ttagggggca tgaaagatga ctttataacc ttgctcaccc caatgttgta
     1021 aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca gacaatcaat gtttgcgtcg
     1081 ggaacagttc cgggcgcggc gattcaatgg gatcagcaag aacagaaatg tgccatcctg
     1141 acaatgataa acgccatgct tccagccaca atcgtgctct ctcttcaaca ttccacgcca
     1201 tcagaatggc ttctttaccg ggcttacggc gcatttcgaa aagcgaggtt gctgcgtatt
     1261 caattaatgc gccgtcaaac atactgctca taatgcggga ggtgttgtga tcaagcacga
     1321 gacgctggcg aacaggaagg taaacatgat taatcaattg atctactggg tactctcgac
     1381 ccagtgaaat aattctcgcg cgtagtttgg caggattagc catgcgaaga attgacatca
     1441 tctcttcttg caggcggctc cagtcatctt ccgtatcctg gctggtggtt tccagtaatg
     1501 ctttaacttt gcctacaggg acgccattac ttatccaacg cttgatctct tcgatgcgtt
     1561 gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact gcgctgtggt tttaacaaac
     1621 cgtagcggcg ttgccaggcc cggagagtga caggattaat cccgcaacgt tcagcaacat
     1681 caccaatgct gtaataagcc acaattcctc cttgcggtca caaatctccg tcgcctgtac
     1741 acgacccaat aatactttgt acaatatacg ctaaaattgt acaaagtata aataa
//
LOCUS       DAZUFB010000293         1790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZUFB010000293 DAZUFB010000000
VERSION     DAZUFB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.301"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..812
                     /locus_tag="ACHY3M_005345"
     CDS             120..812
                     /locus_tag="ACHY3M_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4320028.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            835..>1790
                     /locus_tag="ACHY3M_005346"
     CDS             835..>1790
                     /locus_tag="ACHY3M_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4320029.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVT"
BASE COUNT          422 a          437 c          442 g          489 t
ORIGIN      
        1 tttttcagca aaagccccgt taatgtgcca taaaacaagc aatttgccgt ttttgcaacc
       61 gtaatcacac ttcctggtgg catactaagg aggaataaaa agaaaaagga agattagtaa
      121 tgcctttaag cgcacaacag ttagctgcac agaaaaacct ttcgtatgtt ctggctgaga
      181 agctggcgca acgcatctta aaaggtgaat atgaacccgg caccattttg cctggtgaga
      241 ttgagctggg cgagcaattt ggagtgagtc gtacagcggt acgcgaagcg gtcaaaacgt
      301 taacggcaaa agggatggtt ttaccgcgac cgcgaattgg tacccgggtc atgccacaat
      361 caaactggaa ttttcttgat caggaattgc ttacctggtg gatgacagaa gagaactttc
      421 atcaggtcat cgatcacttt ctggttatgc gtatctgtct ggagccgcaa gcttgcctac
      481 tggctgcaac ggttggcacc gcggaacaga aggcgcatct caatacgtta atggccgaaa
      541 tggcggcatt aaaagagaac tttcgccgtg aacgctggat cgaagtcgat atggcctggc
      601 atgagcatat ctatgaaatg agcgccaatc cgtttttgac ctcattcgcc tcgctattcc
      661 attcggttta tcacacttac ttcacgtcaa ttaccagcga cacagtgata aagctggatc
      721 tgcaccaggc gattgtcgat gcgattgtcc aaagcgatgg cgacgcggca tttaaagctt
      781 gtcaggcgct gctacgctca cctgataagt gacaacccga taataggaca ttgaatgagc
      841 gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg cggcgatggc cttcttcatg
      901 caggcacttg atgccactat tctgaatacc gccttacccg caatcgctca tagccttaat
      961 cgttctcctc tcgcgatgca atcagccatc atcagttata cgctgacggt ggcgatgctt
     1021 attccggtaa gcggatggct agccgatcgc ttcggtacgc gtcgcatttt tacccttgcc
     1081 gtgagtctgt tcacattggg ttctctggcc tgcgcacttt ctaattcgct accacagctg
     1141 gttgtcttcc gggttattca ggggataggc ggcgcaatga tgatgcctgt tgctcggctg
     1201 gccttactgc gcgcttatcc tcgtaatgaa cttcttccag tattgaattt tgtcgccatg
     1261 ccgggtctgg tggggccaat tttaggcccc gttcttggcg gcgtgctggt cacctgggca
     1321 acctggcact ggatattttt aatcaatatc cccataggta ttgcgggcct tctttacgcg
     1381 cgcaaacata tgcccaattt caccaccgca cgacgcagat tcgatatcac tggctttttg
     1441 ctgtttggcc tcagccttgt tctcttctca agcggaatag agctattcgg ggaaaagatt
     1501 gtcgccagct ggattgcctt gacggtaatt gtcaccagca tcgggttact gcttctctat
     1561 attctccatg cgcgacacac gccaaaccca ttaatttcat tagatttatt taaaacccgc
     1621 actttctcga tcggtatcgt aggcaatatt gcaacccgtc tggggaccgg ttgtgtaccg
     1681 ttccttatgc cattgatgtt acaggtagga tttggttatc aggcgtttat tgccggctgt
     1741 atgatggcac cgacagcgtt aggttccatt attgcaaaat cgatggttac
//
LOCUS       DAZUFB010000294         1786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZUFB010000294 DAZUFB010000000
VERSION     DAZUFB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.302"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..1354)
                     /locus_tag="ACHY3M_005347"
     CDS             complement(167..1354)
                     /locus_tag="ACHY3M_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFS4320030.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKICFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1354..1719)
                     /locus_tag="ACHY3M_005348"
     CDS             complement(1354..1719)
                     /locus_tag="ACHY3M_005348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320031.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
BASE COUNT          423 a          525 c          422 g          416 t
ORIGIN      
        1 aaaaagatta aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga
       61 gtgcaccgcc gtcaattaaa tggatcttat cgattgatac tgcatatcac tgatggtttt
      121 ttaccccatt cccctgaaaa tggacgcaac ttctccgatg cggctctcag ccgcacaccc
      181 gctgttgcgc cagtggctca tgcatcagga ccagctctgt cagacggtag ccccgcttca
      241 gccccgtaaa acgcatctga cctccgcaca ggatgcactt cagcgggtca accttcagta
      301 accgctgata catccctctc cacctgattt gcatcgccgt ctttctcacc gtctccgtta
      361 tgacgtacac aacatcttcc agtaaccggc gcttcaccgg actcaggaaa ccgtagtacc
      421 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccata aactcatcac
      481 cactcatcac caggtattct tcccgttttg tccggtgact gttgtaacgc agaccaattt
      541 catcctgacc agcatagtgc tccagacggc tcatcggcac cggcggtttt ttcaggtaag
      601 agccgaagta caccgccaca tgtgtggcgt tatccatcac ccgggatacg ttgacattcc
      661 agccccgccg gtaatgactg tccaggaagc gattccagtc ccgtttactg cttccttctg
      721 ctgccagcgc atccggtatc accaggtccg gatatttccg tgacagtaac cgcgttatcc
      781 ggtaacgcca catactcatc accttacggg cgtaaaaatg aaggtttttc caggtgtggt
      841 ctgatgtcac gccgccggtc gttgtcgaca ggtgaatgtg cggatgccac tgctggtcac
      901 gtccccatgt gtggatgacc gtgaatatcc ccggcaccac atctgcctgg cggcagattt
      961 cctgtatcac atccgcagca atgcggctca tctctgccag taaccaccgg ttgtggaaca
     1021 ccagggacca gtactggcag ggaagtgtga acacaatatg ctgccacgga cagtcgggaa
     1081 ccagactcag cagatactgt atccactgtg cgccagcctt cactccgcag tgcgggcagg
     1141 agcgactttt acaccggaag cagatctttt ttatgtggct gcagtccgga gatgaacagc
     1201 accactgtgt gtaccccatc agcgtggtcc cgcaggccat gatttttgtc acagactcaa
     1261 tgactaccgg acgcactgaa ccttccggtt gtttctccag ccagttaagc cagcggtttc
     1321 cctgctgaaa aatgtcggca aaacggggaa gcatcagaag ggcgggggga ctccgtccgg
     1381 ccagtgaacc gtgccacact ccgggcagta catgccgccg gcgctgatac cggcaagaat
     1441 ggtcgcaaac tcccgctccg tgcagcgggc tatttcagga tacccttcgt catcaacacg
     1501 tacaaaccag aagaccagct ttttgtttcc ggcatccaca aagaagggaa tattcaggtc
     1561 tgcgcagcac tcaacggcat cgtcaaaatt atcaaagcgc agagcttctg cgtcttcttc
     1621 gtcaaagaag tcatcgtcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgagg
     1681 ggcaggtttt cgtttacggg gtttagctga acgggacata taactacctg aaagacaatg
     1741 atattgtctg tttttataac ggtaatttca ggccatgaca agccgc
//
LOCUS       DAZUFB010000295         1773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZUFB010000295 DAZUFB010000000
VERSION     DAZUFB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.303"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..782
                     /locus_tag="ACHY3M_005349"
     CDS             <1..782
                     /locus_tag="ACHY3M_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4320032.1"
                     /translation="YQSIIKGLDRLFEGINLDSAFSLSEEERSYFEEKAKNLNALAQL
                     GISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKIS
                     GYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNII
                     NNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLY
                     LCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            784..>1773
                     /locus_tag="ACHY3M_005350"
     CDS             784..>1773
                     /locus_tag="ACHY3M_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFS4320033.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSP"
BASE COUNT          609 a          273 c          337 g          554 t
ORIGIN      
        1 aatatcaatc aataataaaa ggtctcgata gattatttga aggtataaac ttagattcag
       61 cattctcatt atctgaagaa gaacgctcat attttgaaga aaaagctaaa aatttaaacg
      121 cgcttgcaca gttaggtatt agtgttgaga taatatctca tgaacttgaa gaaatggatt
      181 ctatggtaac cagaggacta aactctcttc ctacttctgt aaaagaacac cctggttttt
      241 cattggcgtt aaatgctcac agatcgctta ctcaacaaat acgtttctta tcacctttga
      301 aaatatcagg ttatcaatcc aggcagagaa taactggaaa aaatatcatg gattatgtcc
      361 tgaagttctt tggggagcgt ttcgaacggc aacgaataac tattgaattt agtgaagagt
      421 ttaagcaaat cgcaataaca gatataccat caaggatcta tcctgttttt actaatatta
      481 tcaacaatgc aatgtattgg gtcagtctgt caaataatag gctcataaag attggttttg
      541 tgaattcttt ggttatcata gcaaattctg gtccggcaat tgataccgat gatatcccgc
      601 gactatttga actattttat agcaaaagag caaatggaca tggggtaggt ctgtatctat
      661 gtcgagaaaa ccttgctgtt gcacatcata aaatatggta ttcagaacct gatgaaggcg
      721 ataactattt aataaaagat ggcgctaatt ttgtgatcca gttcaatgga gtggagttct
      781 aatatgacag tggcaaatta taattctctt gtccagaaaa ctttctgcga aaatgcaatt
      841 cgttccgttg tcatgattga tgacgatttt ctgacgtatt ctgaatcaat cagggcgttg
      901 aataacgaag ttgatttaga ctacaacaaa attgactcat ctaaacgagc cgctactctt
      961 gagagctttt ttcaatctaa aaatatgatt tgtgatgttg acaatggttc tgttaatttc
     1021 gatgtggatc ggattagaaa atcagatctt attattgtag attatcatct tgataataat
     1081 gcacctgata aaacacttaa actattacaa gatttgaaag actccgatca tttaaatatg
     1141 attgtaatat atactagaga gaatttagaa acggtttgga tgcagatatc atcgactctc
     1201 aaaggtgctc tggatatcaa cagcttgatc attgactacg ataatgaaga tgtccaaagt
     1261 tattgggaag acgttgtatt accgaactta aatgataatg gtaataaagc tctcacaaga
     1321 gatgaaacaa tagcctatat taaagacagt aagccttgta gaagaattaa aagattaata
     1381 catgatgatg ctgtgttgga ggatcaaaag gataaaaact tcattgcaaa aatgattgca
     1441 gaatatgctg tgtctagaaa tgcaattatt tctagcaaca catctggcaa tgtcattcgg
     1501 ggtgatgaaa gcggagtaaa atggattcaa tgtggtaata tctttgtctc cctatttcat
     1561 aaggttcaag atgatcatga aaacgatgga gataggattt ggcaaactct caatgattct
     1621 ctcattgaat ggaaaccatc ttattatcag ttaataaaat ctgaaattca gaatgcaatc
     1681 gaagctgagg ctttatcttt tgtaaatcat ttggctaacg atcattacgg tcaagctgcg
     1741 tggttaaatg agatattaaa atcagactcg cct
//
LOCUS       DAZUFB010000296         1765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZUFB010000296 DAZUFB010000000
VERSION     DAZUFB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.304"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..981
                     /locus_tag="ACHY3M_005351"
     CDS             <1..981
                     /locus_tag="ACHY3M_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320034.1"
                     /translation="HRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTR
                     EDADEEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTW
                     CEEHLKPLTEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYA
                     QQEQVMMYILTRVPALTGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMP
                     KLKAEFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAV
                     ALCMANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            981..>1765
                     /locus_tag="ACHY3M_005352"
     CDS             981..>1765
                     /locus_tag="ACHY3M_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFS4320035.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRT"
BASE COUNT          375 a          475 c          533 g          382 t
ORIGIN      
        1 caccgcatta ccctggatga tgccattgcg gacgggctgt accgtcgtat ctgttatgtc
       61 accggccgcg aatggtcacc ggaaagcgag cagaagtggc gtgatgatct ctacaaaaac
      121 gccccgaccc gtgaggatgc cgacgaggaa tacggctgta tcccgaaaaa atccggcggt
      181 gcctatattc ctcacgcgct tattgaaatg gcgatgatcc gcgacatccc gattctgacg
      241 tttgaagccc cggacgactt catcagccgt gccgcatggc tgcgtgaatc ggaagtttta
      301 acctggtgtg aagaacattt aaaaccgctt acagagaaat taaatccccg ttcccgcttc
      361 agcttcgggg aagattttgc ccgcaccggt gacctgtcct gctttgtgct gctggaaatc
      421 accgaatccc tggcaaaacg cgaggtgttt cgcgtggagc tgcgtaacct gccatatgcc
      481 cagcaggagc aggtgatgat gtacatcctg acccgcgttc cggcgctgac tggcgcggcg
      541 ttcgatgcca ccggtaacgg cggctatctg gccgaagccg cgctgctggc ctttggcccg
      601 gacatcatcg actgcgtgat gttatcgccg aagtggtacg gcgagtggat gccaaaactg
      661 aaggccgagt ttgaagatca gaatatcctc gtcgcccgcc atcagaccac gcttgatgat
      721 ttgcgccatg tgaaggtggt gaacggtatc ccgcaaatcg acaaggggcg cacgaaggac
      781 cagaacgcaa cggctgtgaa cgcccgccgc catggtgatt ttgccgtggc gctctgtatg
      841 gcaaaccggg cgtcatacat ggagggtttc atcctggatg aatcagcctg tcaggcgctg
      901 ccggagcggt cgcgggccat ggagggcggt tatcgtgatg acgatgaggc atatcatgaa
      961 tttgatcggg ggtgctggta gtgggacgca taattgatct tgacggaaaa cctttctcct
     1021 ttgacccgga gatgcagagt gccgtgctgg atattccgca gattgccagc cgttatattg
     1081 aacatccggc ctcgggtatc accccgaacc gggcggcgca gtgccttcgc ggggctgaac
     1141 gtggcgatct gattgcccag tccgatctgg ccgctgacat tgaagaaaag gatacccacc
     1201 ttttcgcaga gctgggcaag cgacgtcttg ccattcaggg cgtgccctgg agtattgagc
     1261 cgccaccgaa cgccagcgcg aatgagaaaa aggacgcgga aatgctcgac gaatatcttc
     1321 attccgccga ctggtttgat gccatgctgt ttgatgccac cgatgccatc ctgaagggct
     1381 attcctgtat ggagattgag cacgggatgc tcggtaaaat gcacatcatc cgcgccatcc
     1441 gctggcgtga cagcgggcat ttctgcctta acccggatga tttgagcgaa ctgcggctgc
     1501 gtgacggcag ccatgccggg gtggcgtttc agccctttgg ctggatagtg catcagtcac
     1561 gttcacgcac cggttacggc ggcgcaacgg ggcttgtcag gacgcttatc tggccgttca
     1621 ttttcaaaaa ctattccgtg cgtgatctgg ctgaatttct ggaggtgtac ggcctgccga
     1681 tgaaggtcgg gaaatacccg tccggggcaa caccggagca gaaaagcgcc ctgatgcggg
     1741 cggtgatgga tatcggtcga cgtac
//
LOCUS       DAZUFB010000297         1765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZUFB010000297 DAZUFB010000000
VERSION     DAZUFB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.305"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1086
                     /locus_tag="ACHY3M_005353"
     CDS             <1..1086
                     /locus_tag="ACHY3M_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4320036.1"
                     /translation="FVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADK
                     MAEMKTSITETRNEITQRVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNG
                     IPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPTFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLS
                     ANQIEGDLVKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNS
                     PNQQSYWYSTCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGA
                     NNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            1155..>1765
                     /locus_tag="ACHY3M_005354"
     CDS             1155..>1765
                     /locus_tag="ACHY3M_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4320037.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGIG"
BASE COUNT          438 a          422 c          525 g          380 t
ORIGIN      
        1 tttgtggaag ccagtggccg ggccagcaat gatgcagaag ggtatctggg gctgtttcgg
       61 gaaaaaatag gaaaactgca tctggctcag gggctgtggg agctgataga caacagccag
      121 cttgcggata agatggcgga gatgaagacc tccatcaccg aaacccgcaa tgaaatcaca
      181 cagagggtca gtaaaacgct ggaggaccag agcgccacca tacagcagat acagcgcgtg
      241 cagaaggaca caaatgatga ccttgctgca ctttacatgc tgaaggtaca gaaaacaaaa
      301 aatggcatac cctatgttgc cggtattgga gcggggattg aggatactga tggccagccc
      361 ctgagcaaca tactgctgct ggctgaccgt atcgcgatga taaatccgga gagcggcaac
      421 agcacgccgt tatttgtggc gcaggggaat cagctgttca tgaacgacgt gttcctgaag
      481 cgactgtttg cggtgagtat cacgtcatcc ggcaaccccc cgacgttttc cctgacgccg
      541 gaagggaagc tgacagccag gaacgcggat atcagcggag caattaccgc gaataccggc
      601 acgctcaata atgtcaccat taacgagaac tgtgtcatca gagggaaact gtctgcaaac
      661 cagattgaag gcgacctggt gaagacggtg gggaaagcct ttccccggaa taacagttat
      721 gccagcggga cggtaaccgt cacagtttac gatgaccagg gcttcgaccg gcagatcatc
      781 attcccccgg tgctgtttcg cgggacgaaa caccagaatt tcaacagccc gaatcagcag
      841 tcgtactggt attccacctg taagctgcag gtgctgaaga acggggttga gattttccat
      901 gaaccggcaa cggatgtcag ccgggtgttc tcatcggtga tagatatgcc ggcagggcgg
      961 ggtcatgtca ccctgacgtt taatgtgtcg tcggccggtg cgaacaactg gacgccgaca
     1021 acgtacatca gtgatttact ggttgtggtc atgaaaaaat ccacggcagg gatcagtatc
     1081 agctgacggt ttattaaccc ggacgggcac ctcaggaggt gcctttttta ttgactgaaa
     1141 acaaagaggt aatcatgcgg catttatacg caacgatatt attgtttact accctgctgg
     1201 caggaattgc ctttcctgca caggctgaaa gcggacacgg tgcattttcc gtgggatatg
     1261 ctcaggttca cccgggcggc gtaccggcat tgtccggtac cggtgcgcgt gcaggtgatt
     1321 taaaagggat taatgtgaaa taccgttatg agttcacgga tcacctgggc ggcattgtcg
     1381 cgctgagtta tgcatcggtg aagaaaagtg acacgatgaa gacgggtgaa aataccttcc
     1441 attatgaaag cctgcgcggt cgttatgtca gtctgatggc cggccctgtc tggcagctca
     1501 gtgagcgggt cagtctctat ggcatggccg ggatggcgta cacccgctgg tctgacagtg
     1561 ttcaggatta ccggcgtgat gaagtgaaac cggggtatgt gaaggagacc accaccgcca
     1621 gtgatggcca tactgcgcgt catctgtcgc cggcctggaa tgccgggatt cagttcagtc
     1681 ccgtagagac ggtggttatt gaccttgctt atgaaggttc cgccagtggc gactggcgca
     1741 ctgacggctt catcgtgggt atcgg
//
LOCUS       DAZUFB010000298         1729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZUFB010000298 DAZUFB010000000
VERSION     DAZUFB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.306"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..534)
                     /locus_tag="ACHY3M_005355"
     CDS             complement(91..534)
                     /locus_tag="ACHY3M_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4320038.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            691..1620
                     /gene="metA"
                     /locus_tag="ACHY3M_005356"
     CDS             691..1620
                     /gene="metA"
                     /locus_tag="ACHY3M_005356"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFS4320039.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          416 a          479 c          389 g          445 t
ORIGIN      
        1 gtcacgatgg cctttttgcg ttggtgaaaa aaatgccggg tgcgacgctg gcgcgtcttc
       61 tccagcctgg gcaggcacgg caggggcggc ttattccccc acatacgcca gattcagcaa
      121 cggatacggt ttccccaaat cgtccacctc agagcgtccc gtaaccttaa aacccacctt
      181 cttatagaac ccaaccgcct gctcattttg ctcattaacg ttggttgtca gttccggtgc
      241 catcgagagc gcatgctcca ccagcatccg acccacgccg cagccgcgca catcaggatc
      301 gataaacagc gcatccatat gctgcccact taacaacata aacccaaccg gctgatcccg
      361 ttcattaacc gcgacccaca acggtgcttc cggcagaaag gaacgaacca gctcttccag
      421 ctcggcccga tactctgctg ataaaaaatc gtgggtggca tcgacagatc gacaccaaat
      481 cgcaacgagt tcctcccctt cctcatgctg tgagcggcga atactaataa ccattttctc
      541 tccttttcgt cattcttata ttctaacgta gccttttcct tgaaactttc tcaccttcaa
      601 catgcaggct cgacattggc aaattttctg gttatcttca gctatctgga tgtctaaacg
      661 tataagcgta tgtagtgagg taatcaggtt atgcctattc gtgtgccgga cgagctaccc
      721 gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga caacttctcg tgcgtctggt
      781 caggaaattc gtccacttaa ggttctaatc cttaacctga tgccgaagaa gattgaaact
      841 gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc aggtcgatat tcagctgttg
      901 cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc atctgaacaa cttctactgt
      961 aactttgaag atattcagga acagaacttt gacggtctga ttgtaaccgg tgcgccgctg
     1021 ggcctggtgg agtttaatga tgtcgcttac tggccgcaga tcaaacaggt gctggagtgg
     1081 tcgaaagatc acgtcacctc gacgctgttt gtctgctggg cggtacaggc cgcgctcaat
     1141 atcctctacg gcattcctaa gcaaactcgc accgacaaac tctctggcgt ttacgagcat
     1201 catattctcc atcctcatgc gcttctgacg cgtggctttg atgattcatt cctggcacca
     1261 cattcgcgct atgctgactt tccggcagcg ttgattcgtg attacaccga tctggaaatt
     1321 ctggcagaga cggaagaagg ggatgcatat ctgtttgcca gcaaagataa gcgcattgcc
     1381 tttgtgacgg gccatcccga atatgatgcg caaacgctgg cgcaggaata tttccgcgat
     1441 gtggaagccg gactaggccc ggaagtaccg tataactatt tcccgcacaa tgatccgcaa
     1501 aatacaccgc gagcgagctg gcgtagtcac ggtaatttgc tgtttaccaa ctggctcaac
     1561 tattacgtct atcagatcac gccatacgat ctacggcaca tgaatccaac gctggattaa
     1621 tcttctgtga tagtcgatcg ttaagcgatt cagcacctta cctcaggcac cttcgggtgc
     1681 cttttttatt tccgaaacgc acctcaggag gtgaataaat tttattcat
//
LOCUS       DAZUFB010000299         1725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZUFB010000299 DAZUFB010000000
VERSION     DAZUFB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.308"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..671
                     /locus_tag="ACHY3M_005357"
     CDS             <1..671
                     /locus_tag="ACHY3M_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4320040.1"
                     /translation="STPEGEWYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRD
                     AYHLAGIDMPDFEREDDWWRNGQNLYLGNMEATGFYRISLPSAQPGDILLCCFGASVA
                     NHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHVSAFTGIYNDLAAA
                     SACM"
     gene            668..1249
                     /locus_tag="ACHY3M_005358"
     CDS             668..1249
                     /locus_tag="ACHY3M_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4320041.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
BASE COUNT          393 a          413 c          544 g          375 t
ORIGIN      
        1 taagcacgcc ggagggggag tggtatatcc cttgtgtgaa tatttctgca gagccggagg
       61 cgtattttcg tatcgcaccg gaagactggc tgcgggcaga gatgcagggg gagattgtgg
      121 cactggtcca cagtcatccc ggtgggctgc cctggctgag cgaggctgac cggcggctgc
      181 agataaaaag cgcactgccc tggtggctgg tctgccgggg tgacattcac aaattccgct
      241 gtgtgccaca tctgacagga cggcgctttg agcacggggt gacggactgt tacacgctgt
      301 tccgggatgc ctaccatctg gcgggaattg atatgccgga ttttgagcgt gaggatgact
      361 ggtggcgcaa cggtcagaac ctgtacctgg gcaatatgga ggcgactggt ttttacagga
      421 tttccctgcc ttccgcacag cctggcgata tcctgctgtg ctgctttggc gcatcggtgg
      481 ccaatcatgc cgccatatac tgcggcaacg gtgagctgct tcaccatctg cctgaacaac
      541 tgagtaaacg ggagaggtat tccgaaaaat ggcaacgacg aacgcattca gcctggcgtc
      601 accgccactg gcacgtatct gccttcacgg ggatttacaa cgatttggcc gccgcctcag
      661 cctgtatgtg aacacggcag cggaagccat ccgtgccctg tcgatgcaga tgccgggatt
      721 ccgccgtcag atgaacgaag gctggtacca gatacgtatt cgcggtgagg acacggcacc
      781 ggaggcggtg tacgcccgtc ttcacgaaca gctgggtgag ggaacggtca tccatattgt
      841 gccgcgactg gccggagccg gaaagggcgg actgcagatt gtgctggggg cggcagccat
      901 cgtgggctct ttcttcactg ccggggcatc aatggcgtta tggggttcag ccctggcagc
      961 cggtggtttt tctgccacca cgatgctgtt ttcacttgga gccagcatga ttctgggcgg
     1021 tgtggcccag atgctggccc cgaaggcaaa aacaccggat taccgcgcaa cggataacgg
     1081 cagacagaac acgtactttt cctcgctgga taacatgatt gcccagggga acccgatgcc
     1141 ggtgccttac ggggaaatgc tggttggctc ccgccgtata tcccaggaca tcagcacccg
     1201 tgatgaaggc gggggcggaa cggtcgtggt tgtcgggcga cagggataaa acataaaaaa
     1261 atcccgcagt gatcgcggag ctgcggggac agacaaatga agatcaatgt taaggagttg
     1321 tttttgttac tcgggcaaaa aaacactaac gcagcgaaat tataagcgcc acagtcagtg
     1381 tgtgaaaatg tgaagatatt cagaattttt atgccattac cggttttaac caacaggatt
     1441 atcggtgggc atgaaagaaa accccggtat ctgctgatac cggggtttct ctttagcatg
     1501 gcagaaatgt gtttcatgct tttcgggcga aggatatccg acttctgtac ggaatggcaa
     1561 gtggcggtta atttattcag gggaaggctg tatgggaaaa ggtggcggta aggcacacac
     1621 gcctcgtgag gcgaaggata atctcaaatc cacgcagatg atgagtgtga ttgatgcgat
     1681 tggtgaggga ccgatagaag gtccggtgaa gggactgcag agtat
//
LOCUS       DAZUFB010000300         1717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZUFB010000300 DAZUFB010000000
VERSION     DAZUFB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.309"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..345)
                     /locus_tag="ACHY3M_005359"
     CDS             complement(25..345)
                     /locus_tag="ACHY3M_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4320042.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            453..626
                     /locus_tag="ACHY3M_005360"
     CDS             453..626
                     /locus_tag="ACHY3M_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4320043.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            697..1619
                     /locus_tag="ACHY3M_005361"
                     /pseudo
     CDS             697..1619
                     /locus_tag="ACHY3M_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
BASE COUNT          480 a          381 c          383 g          473 t
ORIGIN      
        1 cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt ctctcaactt
       61 ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa cctgggcttg
      121 aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt tatctgctat
      181 ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa tctctgagtt
      241 catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc cgtcaggaaa
      301 acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa atctctcaaa
      361 aaaaatcatg gtgccatatt gccatacgat ttcaatagtg gcattatggc cttcatggcg
      421 tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc actgagcgca
      481 aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt cgccgcctgg
      541 cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca aaaggtgtta
      601 gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc aaccattgag
      661 gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc aattgcagat
      721 cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat taacaatgag
      781 ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc tgctcaattt
      841 gttaagttaa aacagaggtt tgtcaagggt atttcggaaa tcgaaatacc ttctgctggc
      901 ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct ttcaagcatt
      961 caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca ggaagagtgt
     1021 gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg caaagctaaa
     1081 aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca actcgtcatg
     1141 agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac catgtggtcg
     1201 tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca gtttaccgaa
     1261 gctctgtcct tgctgctcgc gttccgcttg aaggagagtt aatcggcaaa caagagaaga
     1321 gcaccaacga gctttccgca aaagaagcaa acagccttgt atggctatgg gattatgcca
     1381 accgctcaca ggcattattc cgcgaattgt atccagcgct aaagcaaatt caatcgaact
     1441 attccggcag atgttacaac tacggtcatg agttctcgta tgttattgga atggcgagag
     1501 acgttttaat caatcacaca cgagatgttg atattaatga gccagacgga ccaacgaatc
     1561 tttccgcatg ggtaaggctt aagaacaaag aattacctcc ttcactacat cactactaac
     1621 agattgccaa cgaaatgacc cagcttcggc tgggtttttt atcaggagtt ctcatgctct
     1681 atagcaatat attggcgcac gcccggcgat gtgcgcc
//
LOCUS       DAZUFB010000301         1716 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZUFB010000301 DAZUFB010000000
VERSION     DAZUFB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.310"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..128)
                     /locus_tag="ACHY3M_005362"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="ACHY3M_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(231..1357)
                     /locus_tag="ACHY3M_005363"
                     /pseudo
     CDS             complement(231..1357)
                     /locus_tag="ACHY3M_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            1439..1624
                     /locus_tag="ACHY3M_005364"
     CDS             1439..1624
                     /locus_tag="ACHY3M_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320044.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            complement(1539..>1716)
                     /gene="ydcD"
                     /locus_tag="ACHY3M_005365"
     CDS             complement(1539..>1716)
                     /gene="ydcD"
                     /locus_tag="ACHY3M_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFS4320045.1"
                     /translation="NISKLENMEFCATKQRVYFSDKNKKASYKIINYGDYYDVDYYDN
                     NLKNEVFDWIGKWS"
BASE COUNT          475 a          380 c          234 g          627 t
ORIGIN      
        1 ctttctgcac cagaaataac ggcaaaaata gtcaatagta ggatgcctga caatttatgc
       61 tctactttcc aggcttatct gtaatcggga ataatagaaa tatgttccat caatttttta
      121 agttccattt gttcacctta tttatgtaag gagtatttga tcatgtataa gcaataaaaa
      181 acagcttcag gtaatgagga atatctcaat ttttaaacat aaaatgcgaa ttatttagta
      241 caaaaagtga acttctgatg atctttccct gtgtttaacg cagccttctt tcttttcctc
      301 ttcaccctta atgtctttgc aatcccttaa taaattcagt gccatcttct ttattccaga
      361 tattatttag gctgcgtttc ctcttcttat ccggctggca tcttcattca tttttacatc
      421 taacacccaa tgaagactgt gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc
      481 tttagcatcc atatcctttg atgaaatata atatcggatg cttacacctt ctgctgattt
      541 atcttctttc ttctgcctga atgacaatgc tacacaaagc ttttttaatc ccttccattc
      601 gaattcaaaa tcacaaaagt tcaggcgtta cgttactgac aatatgcaaa cgtgtttctt
      661 ttcttccatg acttatctcc tgcgtactaa acgaatcgcc tttataatta gaaaacacat
      721 ttacaggaaa tttttcctcg aatgcatgat gtaatttccc ctgattgcct tttactgcca
      781 gaagataatc tgctttttta tctttgatct tcgaagcgat atctttctga cagcccatag
      841 catcaatggt tatcaaattt ttctttaaat acagtaggtt aagcaactct ggaatggctg
      901 taatctcatt acttttggct tccgttttca cctgccccag tacaacacca ttttcgttcg
      961 agaatgcact caccatatgg attgctcctt ttctttttcc cttatcaaag gagcctctta
     1021 tggtctttcc atctattgct ataatttcac catcagtgat ttcatggcac tcctgcatcc
     1081 attcaataaa catcttttca aaggccaaac tgtcaatgtt actcacaacg cgtgcaatgg
     1141 tgtcatcgac cggaatgcca ttatcaaaat caccatattt ctttagccat tcaagtcttt
     1201 catgtccaaa atcttcaatt tcctgccact catcggcacc tgcaattact gcacatacgg
     1261 tgagaaacaa aatagcagat aatttatgtt taacctttcc ttgttgtcgt atatcaggga
     1321 tcactgaaat ataatcaagc aaactttgaa tactcacctg gcgtcctttt ttatcttttt
     1381 agttattcca aaggttaaat tttgatcacc tgtaggcgtt aaagccaaga tgaaaatcgt
     1441 gatttacatc acaattttca tgatcttgcc ctgccccgtc ccttgctctc atgtctttct
     1501 gtatgtattt tatatttttc tctttctggt gttgttcatt atgaccattt accaatccag
     1561 tcaaaaactt catttttcaa attgttatca taataatcaa catcgtaata gtctccataa
     1621 ttaattattt tataactagc cttcttgttt ttatcactaa aatacacccg ttgttttgta
     1681 gcgcagaact ccatattctc cagtttacta atgttt
//
LOCUS       DAZUFB010000302         1706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZUFB010000302 DAZUFB010000000
VERSION     DAZUFB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.311"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..854
                     /gene="queE"
                     /locus_tag="ACHY3M_005366"
     CDS             183..854
                     /gene="queE"
                     /locus_tag="ACHY3M_005366"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFS4320046.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          439 a          377 c          415 g          475 t
ORIGIN      
        1 gttttgacat gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt
       61 taattattaa cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata
      121 agacatgatt gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg
      181 ctatgcagta cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg
      241 gcgtccccgc catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca
      301 ccaaacacac ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga
      361 agaccaaaga gagtgataag tggggggcgg cgagcagtga agatttgctg gctgtcattg
      421 gtcggcaggg atataccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg
      481 atttgctgcc actgactgac ctgctcgaaa agaacggttt tagctgccag atcgaaacca
      541 gtggaactca tgaagtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga
      601 acatgcgcgg cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc
      661 atccagtggg gcgcgtacgt gatattgaag cactggatga actactggca acgctgaccg
      721 atgataaacc gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt
      781 tgtgcattga aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc
      841 taaatattgc ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt
      901 ctactcattt atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta
      961 atcattagca ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg
     1021 cggtaaagtt tggtagattt tagtttgtat agagttattt taaatattta cctttttaat
     1081 caatggatta agtgctcttt aacataatgg atgtgttgtt tgtgtgatac tataaagttg
     1141 gtagattgtg actggcttaa aaaatcatta attaataata ggttatgttt agagtgttcc
     1201 ccgcgccagc ggggataaac cgcaagatct gccgcgagtg gtttcacccg gcaggtgttc
     1261 cccgcgccag cggggataaa ccggatattt gggcgtaata ccgcccggat agtgtgtgtt
     1321 ccccgcgcca gcggggataa accggtgagc cattacagcg tgtacaggcg gtcacggtgt
     1381 tccccgcgcc agcggggata aaccgccgct aatgcgcgta tctcatggaa ggttggagtg
     1441 ttccccgcgc cagcggggat aaaccgttct tgcgggtgtt gcaaatattc ttcacgtaga
     1501 gctccccgcg ccagcgggga taaaccggag cctgacgaga ctactgaggc cgttctgtcg
     1561 agttccccgc gccagcgggg ataaaccacc atataacccg ttatctcttt ctcaagtttt
     1621 tatattagca gtacttgtaa taagcaacat atccacgtaa cacctcatgt tcaaaatagt
     1681 tctccatgcc agagaggttc acaatt
//
LOCUS       DAZUFB010000303         1702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZUFB010000303 DAZUFB010000000
VERSION     DAZUFB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.312"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..520
                     /locus_tag="ACHY3M_005367"
     CDS             <1..520
                     /locus_tag="ACHY3M_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFS4320047.1"
                     /translation="ATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAI
                     SVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRI
                     EAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNE
                     IITSAIDKLKRS"
     gene            548..1506
                     /locus_tag="ACHY3M_005368"
                     /pseudo
     CDS             548..1506
                     /locus_tag="ACHY3M_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1518..>1702
                     /locus_tag="ACHY3M_005369"
     CDS             1518..>1702
                     /locus_tag="ACHY3M_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFS4320048.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFR"
BASE COUNT          531 a          318 c          405 g          448 t
ORIGIN      
        1 agctacggca atcgagtcaa cggctcagcg atgcaagaaa aacccaattt attcacctaa
       61 aatagtagaa caatctcaaa ttactgcttt tgataaatcg actaatgtca gacgtgctac
      121 agcatttgct atttctgtta tcaatgataa agcaacaatt ccactattga ttaatctgtt
      181 aaaagatcca aatggagatg tcagaaactg ggccgcattt gcaataaata tcaataaata
      241 tgataatagt gatattaggg attgttttgt ggagatgctt caggataaaa atgaggaagt
      301 ccgtattgaa gcaataatcg gactttccta cagaaaagat aaaagggttt tatctgtttt
      361 atgcgatgag ttaaaaaaaa atactgttta tgatgatatc attgaggctg cgggtgaatt
      421 aggtgataaa acgctacttc ctgttttaga tactatgttg tacaagtttg atgacaatga
      481 aattataact tccgctattg ataagctgaa gcgttcatag cgtgagttgc ctatgcacag
      541 tgggggattc ccgccggcac ggtgcagatg ctcgaccggc tggaaagtga aatcctggct
      601 gaccgggtga gtgaggaaag ccgccgctgg ctggcatcgt gcggcctgac cgtggagcag
      661 atgaaaaacc agatggaccc agtgtacacg ccggcgcgaa aaatccacct gtaccactgc
      721 gaccatcgcg gcctgccgct ggcgctcatc agcacggaag gggcaacagc gtggtgcgca
      781 gaatatgatg aatggggcaa cctgctgagt gatgagaacc cgcatcatct gcagcagctc
      841 attcgcctgc cgggtcagca gtatgatgag gagtccggcc tgtattacaa ccgccaccgc
      901 tattatgacc cgctgcaggg gcggtatatc actcaggatc cgattgggct gaagggggga
      961 tggaattttt atcagtatcc gttgaatccg gtcataaatg tagatccgca aggtttggtt
     1021 gatataaatt tataccccga aagtgatctt atccattctg tagctgatga gattaatatc
     1081 ccaggcgttt tcacaatcgg ggggcatggt acccccacat ctattgaatc cgcaacgcgc
     1141 agtatcatga cagctaaaga tctagcatat ctaattaaat ttgatgggaa ttataaagat
     1201 gggatgacag tttggttatt ttcttgtaat acaggtaaag gacaaaattc atttgctagc
     1261 caattagcta aagagttaca tacaaatgta ataggacctg acacgctatg gacgtggtgg
     1321 gggcgaggaa ctaatggtaa gttaaaaatg gatacagtgc taacagcacc aacgaacctt
     1381 aattcaaata aggatctaat ggctataaca acaaaagacc ttggtaattg gataacatat
     1441 gggccatctg ggcaccccat ttctaatatg caaggtacgc cagaaaaacc cagtgatata
     1501 agataggttg tagatgtatg aaagcatgct tgttactatt tttttatttt tctcttattg
     1561 gtcaattgca tggtgctgat gtgaaaataa aagaaaacga aagtgtgatg ggatctacag
     1621 caatgaccta tgatttaagt gaagaaaagc tgatgaaact gaagtataaa tcacaacatg
     1681 gtgattcaga ggcatcattt cg
//
LOCUS       DAZUFB010000304         1698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZUFB010000304 DAZUFB010000000
VERSION     DAZUFB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.313"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..268)
                     /locus_tag="ACHY3M_005370"
     CDS             complement(<1..268)
                     /locus_tag="ACHY3M_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis family protein"
                     /protein_id="HFS4320049.1"
                     /translation="MVLTVKKILAFGYSKVLPPVIEQFVNPICIFIITPLILNHLGKQ
                     SYGNWILLITIVSFSQLICGGCSAWIAKIIAEQRILSDLSKKNAL"
     gene            complement(307..1473)
                     /locus_tag="ACHY3M_005371"
     CDS             complement(307..1473)
                     /locus_tag="ACHY3M_005371"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS4320050.1"
                     /translation="MITYPLASNTWDEYEYAAIQSVIDSKMFTMGKKVELYEKNFADL
                     FGSKYAVMVSSGSTANLLMIAALFFTNKPKLKRGDEIIVPAVSWSTTYYPLQQYGLKV
                     KFVDINKETLNIDIDSLKNAISDKTKAILTVNLLGNPNDFAKINEVINNRDIILLEDN
                     CESMGAVFQNKQAGTFGVMGTFSSFYSHHIATMEGGCVVTDDEELYHVLLCLRAHGWT
                     RNLPKENMVTGTKSDDIFEESFKFVLPGYNVRPLEMSGAIGIEQLKKLPGFISTRRSN
                     AQYFVDKFKDHPFLDIQKEVGESSWFGFSFVIKEGAAIERKSLVNNLISAGIECRPIV
                     TGNFLKNERVLSYFDYSVHDTVANAEYIDKNGFFVGNHQIPLFNEIDYLRKVLK"
     gene            complement(1470..>1698)
                     /locus_tag="ACHY3M_005372"
     CDS             complement(1470..>1698)
                     /locus_tag="ACHY3M_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320051.1"
                     /translation="NAGLGYDYSINDYYKIIAEEIGYTGSFSHDLTKPTGMKRKLVDI
                     SLLNKIGWSSHFELRDGIRKTYNYYLENQNK"
BASE COUNT          548 a          333 c          242 g          575 t
ORIGIN      
        1 gtaaagcatt tttttttgat aaatcactaa gaattctctg ttctgcaatg atttttgcaa
       61 tccatgcgga acatcctcca catattaact gagaaaaaga tacaatagta attaataaaa
      121 tccaattacc atagctttgc ttacccaggt ggttgagtat tagtggtgtg ataatgaaga
      181 tgcaaattgg attgacaaac tgttcaataa ccggtggtag tactttagaa tagccaaacg
      241 ctaaaatttt tttcactgtt aataccatct taaaggcact ctattcgaaa tagagtgcct
      301 cgttagttat tttaatactt ttcgtagata atctatttca ttaaacaaag gtatctggtg
      361 gtttccgaca aaaaaaccat tcttatctat atattcggca tttgctaccg tatcatgtac
      421 agagtaatca aaataactca aaacacgttc atttttgaga aaattcccag taacaattgg
      481 tcggcattca atgcctgctg agatcagatt atttactaaa ctcttcctct caatagcagc
      541 tccctccttt ataacgaagg aaaaaccaaa ccagctactt tcaccaactt ctttttgtat
      601 atcaaggaat ggatgatctt taaatttatc tacaaaatat tgtgcattgg aacgtctggt
      661 ggatataaaa cctggtaact ttttaagttg ctctatccca atagcaccac tcatttcaag
      721 tgggcgaaca ttgtatcctg gtaaaacaaa cttaaacgac tcttcgaaaa tatcatcact
      781 cttagtacct gtaaccatat tctcttttgg taaatttctt gtccaaccat gagctcgaag
      841 gcacaacaat acatgataca gctcttcatc atcagtaact acgcagcccc cttccattgt
      901 agctatatga tgagagtaaa aagaactaaa ggtacccata actccgaatg tgcctgcctg
      961 cttattttga aagaccgcgc ccatcgactc acagttatct tctagtaaga taatatccct
     1021 attatttatt acctcattta tttttgcaaa atcattagga ttacctaata aatttactgt
     1081 caatattgct tttgttttat ctgaaatagc atttttcaaa ctatcgatat caatatttaa
     1141 agtttcttta ttgatatcga caaacttcac ctttaagcca tactgttgca gagggtaata
     1201 tgtcgtagac catgacactg caggtactat tatttcatca cctcttttaa gttttggttt
     1261 attagtgaag aaaagggcag caatcattaa cagattagct gtagaaccag agctaaccat
     1321 tacggcatat ttgctaccaa acaaatcagc aaaatttttc tcatataact caaccttttt
     1381 acccatggta aacatttttg agtcaattac tgactgtatt gctgcatact catattcatc
     1441 ccaagtatta ctagcaagtg ggtatgtaat catttatttt gattctccaa gtaataatta
     1501 taggtctttc tgatgccatc tctgagttca aagtgacttg accaaccaat tttattaagc
     1561 aatgaaatat ctactagctt ccgtttcatt cctgttggtt ttgttaaatc atgagaaaaa
     1621 ctcccagtat aaccaatttc ttctgcaatt atcttataat agtcattaat tgaataatcg
     1681 taacctaaac cagcattt
//
LOCUS       DAZUFB010000305         1688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZUFB010000305 DAZUFB010000000
VERSION     DAZUFB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.314"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..88
                     /locus_tag="ACHY3M_005373"
     CDS             <1..88
                     /locus_tag="ACHY3M_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320052.1"
                     /translation="HNALDDARYQAKYVSVIWQKLIPSQADS"
     gene            161..412
                     /locus_tag="ACHY3M_005374"
     CDS             161..412
                     /locus_tag="ACHY3M_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS4320053.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            447..>1688
                     /locus_tag="ACHY3M_005375"
     CDS             447..>1688
                     /locus_tag="ACHY3M_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFS4320054.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKENEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLKEYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEF"
BASE COUNT          551 a          336 c          391 g          410 t
ORIGIN      
        1 ccataatgca cttgatgacg cccgttacca ggcaaaatac gtttcagtta tctggcaaaa
       61 actgatcccg agtcaggctg attcttaatg ttcaactgtc gccggttgtg actggtattc
      121 tgcaaccggc gctcgtctga tgtaagagat aaagaaatcg atgagcgaag taatcatgat
      181 tgtctctccc ggcaaatggg tatccgaaga gcagttaatt gcgctgaaag gaataaaaaa
      241 aggtacgtta aaaaaggccc gggaaaaatc gtttatggaa ggaagggaat ataagcatgt
      301 cgctcatgac ggtatgccat gggataacag tccatgcttt tacaacctgg aagaaattga
      361 tcgctggatt gagcgccagg catcagcgag accaagacgt catcttactt gactaaaagc
      421 cacactaact aatgagagaa gttgaaatga aatatccgac aggcgtggaa aaccatggag
      481 ggaaattacg tatctggttt gtttataaag gcgtaagagt cagggaaaat ctgggggttc
      541 ctgacacagc aaaaaacagg cgcgttgcag gtgaactacg ctcctctgtt tgttacgcaa
      601 taaaaactgg tgttttcgac tatgcaaaac agtttccctc ctcacgcaat ctggaaaaat
      661 ttggtgaggc ccgacaagat ttaaccataa aagaactggc tgaaaaattt ctggcactga
      721 aagaaaatga agtcgccaaa acatcactca acacataccg tgccgtcatc aaaaatatcc
      781 tgagcataat cggtgaaaaa aatcttgcct catcgattaa taaagaaaaa ttactggagg
      841 ttcgtaaaga gctactgact ggataccaga tccccaaaag taactatatt gttacacaac
      901 cagggagatc ggctgtaact gtaaataatt acatgacaaa tcttaacgcc gtgttccagt
      961 ttggtgttga taacggttac ctggcagata atccgtttaa ggggatctcg ccattaaagg
     1021 aatcaagaac cattccggat cctctttcgc gggaggaatt tatccgtctt atcgatgcgt
     1081 gcagaaatca gcaagcaaaa aatttatggt gtgtttctgt ttatactggc gttcgccctg
     1141 gtgagctgtg tgcacttgga tgggaggaca tagatctgaa aaatggaaca atgatgatca
     1201 ggagaaattt agcaaaagac cgtttcacgg taccaaaaac acaggcggga accaatcggg
     1261 tcattcatct tattaagcca gcaatcgacg ctctccggag tcagatgaca ttaacgagac
     1321 tgagtaaaga gcatatcatt gatgttcacc tcaaagagta tggcagaaca gaaaaacaaa
     1381 aatgcacctt tgtttttcaa cctgaagtgt cagcgagagt aaaaaattat ggtgaccatt
     1441 ttaccgttga ctcaataagg cagatgtggg acgcagcgat aaaacgtgcc ggactccgcc
     1501 atcgaaaatc atatcagtcg agacatactt atgcctgctg gtcgctgaca gctggtgcta
     1561 acccggcatt tatagcaaac cagatgggcc atgcagatgc gcaaatggta tttcaggtat
     1621 acggaaaatg gatgtctgaa aacaataatg cacaggtagc tttgttaaat acacagttaa
     1681 gcgagttt
//
LOCUS       DAZUFB010000306         1674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZUFB010000306 DAZUFB010000000
VERSION     DAZUFB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.315"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..319)
                     /gene="gadE"
                     /locus_tag="ACHY3M_005376"
     CDS             complement(<1..319)
                     /gene="gadE"
                     /locus_tag="ACHY3M_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFS4320055.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKH"
     gene            complement(1118..>1674)
                     /gene="hdeD"
                     /locus_tag="ACHY3M_005377"
     CDS             complement(1118..>1674)
                     /gene="hdeD"
                     /locus_tag="ACHY3M_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFS4320056.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          544 a          353 c          306 g          471 t
ORIGIN      
        1 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
       61 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
      121 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
      181 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
      241 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
      301 tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct
      361 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
      421 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
      481 cgcactccat atattgatcc taagtaagat tcctggttgt tatcaatttg taaatatcaa
      541 acaaaaagtt aacgtttgcg gattactggc caagaataag ttatcaataa ccagtttgtg
      601 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
      661 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
      721 gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
      781 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
      841 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
      901 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
      961 aaaaaattag atgaaattca gtaggttgaa atgatcacta gcaggtaatt atttcaatga
     1021 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     1081 catcaagtga ctatgatcct ggtgacaacc gggataatta ttgctgctta acgaacaaac
     1141 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     1201 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     1261 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     1321 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     1381 tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     1441 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     1501 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     1561 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     1621 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gcct
//
LOCUS       DAZUFB010000307         1662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZUFB010000307 DAZUFB010000000
VERSION     DAZUFB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.316"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..>1662)
                     /locus_tag="ACHY3M_005378"
     CDS             complement(30..>1662)
                     /locus_tag="ACHY3M_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS4320057.1"
                     /translation="NSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLA
                     PDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTD
                     LIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRR
                     ERDLTGWMSLSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGER
                     EKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGL
                     TVEQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENP
                     YYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPII
                     RMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLS
                     DICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVD
                     VDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          326 a          528 c          399 g          409 t
ORIGIN      
        1 aaatatatat tttttcatat ttcctcctgt taattgaccc agcattcttg ccacgttcta
       61 caagccagag tcataactga agcgtcaaca cctgcaccga taccaacaga ttctgtcgat
      121 gattctggat tatcaacatc cactacataa gtatacgcaa aatgcccccc aactccgccg
      181 ccaaccgtta cacctactga attgctgact cccgatgtag gtaaagactt ggtctcagaa
      241 tatgtgatag ccgcatttat cccaccacca attcctgcat gtccgcatgc tgtagcataa
      301 atacaaatat cagataacat ttcacctttc tttttagcaa acgcaacgcc aagtcctgca
      361 tgaccaccta atgatattag atctccactg ccagtaatat caattgatga tgttccatat
      421 gaatcatcat gccaaacatc atataataat tgatataaat tatacaaacc caaaggatcc
      481 atccttatta tcggattcag tgggtaatta tacaggttcc atccccccgc cagcccaatg
      541 gggtcttgtg taatatacct cccctgtagc gggtcatagt accggttccg gttatagtac
      601 agccctgatt cctcatcatg ctgctgcccc ggcagacggt atggctggtg caggtaatac
      661 gggttctctt cattaagctg gttgccccat tcatcatact ccccgcgcca cgccgtattg
      721 ccgtcttcgc tgatgagcgc cagcggcagg ccccggtggt cgcagtggta aaaatgaact
      781 tttcgcgccg gtgtgtattc cggctccacc tgtctggcca gttgctccac cgtcagcccg
      841 cactgcgcaa gccacgcccg gctttcactg ctcacgcggt ctgcccggat ttcttcctcc
      901 agcctgtcca gcagccgcac cagttcagcc ggaaatacca cgccgtgccc gtcctcgctc
      961 ccttcctgct gcagcttctc cgccaggctg cggcgctgcg ctttctcccg ctcgccgttg
     1021 tctgtttcga tgcggatgag cggcgcgaag ctccccggct gatataccgt ctggatacgc
     1081 gtggtgtcgg tctgtaccgt cgtcagccgg tcgccgtccc acccgtacca cgtcatctcc
     1141 ggtttacgcg acagcgacat ccaccccgtc aggtcacgct cccgccgcca tacccgtttc
     1201 gccattcgcc gtcccagcgg gtcgtagagg tagcggctct cgaccagtgg ctcgccatgc
     1261 tgtatccgcg tgtagaacac cagacggtgc tggctgtcgt agtggtagtg gtgggtccgc
     1321 tcgtcgtccg tccggataac acccgccggg ataaggtccg tcttctccgt cagtctgccg
     1381 tattcatcat agtggtaaac atagtgcgca tcctctgcga tgcggttatc cggccacgcc
     1441 gtgagggtac tgtccgggtg cagctccggg tccggcaggc ggtttcccgc cgggtcggtg
     1501 gcatacggga tgcggatgtc caggtccggt gcgagggtac gcacgctctc cagcctgccc
     1561 gtggcgctgt agccgtattc ccgcgtctgt cgcgggccgc tgatgcgcac caggtcgccg
     1621 ttgtcattcc acccgtagtc acggtcatac accaggctgt tc
//
LOCUS       DAZUFB010000308         1621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZUFB010000308 DAZUFB010000000
VERSION     DAZUFB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.317"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..332
                     /gene="ygeI"
                     /locus_tag="ACHY3M_005379"
     CDS             114..332
                     /gene="ygeI"
                     /locus_tag="ACHY3M_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFS4320058.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            415..917
                     /locus_tag="ACHY3M_005380"
                     /pseudo
     CDS             415..917
                     /locus_tag="ACHY3M_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(936..1568)
                     /locus_tag="ACHY3M_005381"
                     /pseudo
     CDS             complement(936..1568)
                     /locus_tag="ACHY3M_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          565 a          305 c          244 g          507 t
ORIGIN      
        1 acagagataa aaatatctaa gatattcacc ttattgcaag atatttaaaa tgctctagag
       61 ttaaaagtat gatgtaaaga ccattggcct agacaattaa aaaagggagc tttatgacta
      121 atccaatcgg tatcaataat ttatctcaaa gttcaaatat agcgaatgca acgggtgatg
      181 aagtcgtatc attagataaa cacatcaaca ccagtgctac tgatacagat caaatacaag
      241 catttatagt ttcaacatgg atggcgcctt ttcaaaatga tatgtattca gaagataatc
      301 ctatctcacc ttactataaa attgaatggt agacgattga ccaaataaat cataacgaga
      361 tacttttata atagtatctc gtccctttca ttcaatcctc acacatgaaa aaatatgcgc
      421 tcaccaaaag ttaaattctt aaccatcttc acgttttgta tatttattac aaaaatgtct
      481 ttcgccagca actcttgctc taatgaagca ggaacaatgt tcagaatcga accaaattta
      541 attaaagcta tcgctcttgt tgaaagcaat ctaaaaaagg atagcatagg caagaacaga
      601 gataagaata acaatataaa gagtttagat tattggttaa tgcagataaa ccagatgcat
      661 attcccctgt taaaaaaacg tggaataata aaagatgaac gagatttgtt agataaccct
      721 tgtctgaaca ttaaaatagg aaccgagatt ttatataacc atttttcacg ttgtggagta
      781 acgtggcaat gcctggggac ttataatgcg ggttttgcaa tggataacca aaaaaagaga
      841 cagcaatatg ccccaaaata tacattgtat ataccaggct taatgaacta gacaatcgta
      901 aggcttcagc aaaatgagcc taaagcctct tttttttata tagtgcaaac acccatacgt
      961 aaagctgttt tgatcaactc gataccactg taaacatcta atttcttcat gatattgagt
     1021 ctatgagttt cgattgtttt tctgcttaac tgcaagaaat tagcgatttc tttgtttgtt
     1081 ttaccatcag ctatgagttg taatatttct gactcacgat ttgtaagttg attatcagag
     1141 gaatacctgg aagatatttt tccacaatcc atatgtacac tatcaaaata agtgtaacca
     1201 ttactaatcg aatcaatagc acgatttagt ttcgccgggt gactggtttt ccatacgcat
     1261 cctttcgcac ctgcttcaag caattttatt gaatcaatat acggcatctt tgctacaaat
     1321 gtaataatat gagcatctat cttatgcgat ttaacccatt ttacgagttc ggaccccatc
     1381 attccacctg caaattctcc taaaaaaaat atttaggctg tagatttgtt acactccgct
     1441 atagctttct tcagatcttt atagccccca acaacctcat ataaatcagg ataatgtccg
     1501 agaaatccaa ttatcccatc aatcataaac ggctgctgat ctgaaactac aattttaatt
     1561 tttcccatat attcatcctg agtgtaaatg ctttaacgca ttcatttata atcatccctt
     1621 c
//
LOCUS       DAZUFB010000309         1601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZUFB010000309 DAZUFB010000000
VERSION     DAZUFB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.318"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..170)
                     /locus_tag="ACHY3M_005382"
                     /pseudo
     CDS             complement(42..170)
                     /locus_tag="ACHY3M_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(201..1363)
                     /locus_tag="ACHY3M_005383"
     CDS             complement(join(201..1102,1102..1363))
                     /locus_tag="ACHY3M_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFS4320059.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWWRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          362 a          434 c          382 g          423 t
ORIGIN      
        1 ttgatttaga gacactggct ttctgaatcg ccacggataa tctagacact tccgagccgt
       61 tgataatact ggttttcata ttctgtcggt gacatctgtt cgctagtacc atgccgacgc
      121 ttactgttat aaaacatttc gatgtaatca aaaatatcac tgcgggcttc tgatcctacc
      181 cacgtaatat ggacacaggc ctaagcgagg ttcttgtttt caaattgttc cggactgagg
      241 ccgccacacc aactgtgccg ccgccaccga ttgtaatcac attcgatata attaaatacc
      301 gttgcccgca ttatttcccg gctgataaag tgttctccat ggatacattc cactttcagc
      361 gaatgaaaga agctttccac gcaggcatta tcgtagcagc aaccttttgc gctcatactt
      421 ccacgcagat tatgccgctt cagttgcgcc tgataatctg ctgaacagta ctggcctcca
      481 cggtccgtgt gaacgataac gttccggggc ctcttacgcc accacagcgc catctgcagg
      541 gcatcgcagg ccagttgcgc cgtcatgcgt ggcgacattg accagccaat aacggcacgt
      601 gaccacaggt caatgaccac tgccagatac agccagcctt catctgtacg taagtacgtg
      661 atgtctcctg cccacttctg gttcgggcca ctggcgtaaa aatcctgctc caacagattt
      721 tctgacacag gcaggccgtg tgcgcggtag ctgaccgggc tgaacttccg ggaggccttt
      781 gccctcagtc cctgacggcg caggcttgcc gccacggttt ttacgttaaa ggggtaaccc
      841 tgagcacgca gttcatccgt caggcgtggg gcaccgtaac gctgttttga ccgggtaaaa
      901 gccgcgagga caacgctgtc gcagtgttgg cggaactgct gacgcgtgct tatccttgtc
      961 cgccgctgac accacgtata ccagccgctg cgggccaccc ggagcacgcg gcacattgct
     1021 ttgatgctga actcagcctg atgtttttca ataaagacat acttcatttc aggcgcttcg
     1081 cgaagtatgt cgcggccttt tggaggatag ccagctcttc atcccgttct gccagctggc
     1141 gtttgagacg tgcaatctcg gtagacatct ccagttcacg ttcagaagac gtctgctgat
     1201 tttgctgttt actgcgccag ttgtagagct gtgattcata caggctgagt tcacgggctg
     1261 cggcagtaac accgatgcgt tcagcaagct tcagggcttc actgcgaaat tcaggcgaat
     1321 gctgtttacg gggtttttta ctggttgata ctgtttttgt catgtgagtc acctctgact
     1381 gagagtttac tcacttagcc gcgtgtccac tattgctggg taagatcata tctccccggc
     1441 agccttcagg aaaatattat cagataactg cgtaaaaaaa gaacaaatgg ttgtgtccgc
     1501 tattgccagt acacctgagt ttacagcctt tcagattatc acaatcaata gcgaacagac
     1561 ttttgagcta tgtgcgcttt taacaaaacg cccctttctc c
//
LOCUS       DAZUFB010000310         1586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZUFB010000310 DAZUFB010000000
VERSION     DAZUFB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.319"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHY3M_005384"
     CDS             <1..80
                     /locus_tag="ACHY3M_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320060.1"
                     /translation="GWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            386..964
                     /locus_tag="ACHY3M_005385"
     CDS             386..964
                     /locus_tag="ACHY3M_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFS4320061.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(924..>1586)
                     /locus_tag="ACHY3M_005386"
     CDS             complement(924..>1586)
                     /locus_tag="ACHY3M_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFS4320062.1"
                     /translation="ITHYINHLGIPLSNIDIIIDLRDELTEDKINSGELYTLAMGLIN
                     NLAHLNEYRKVILSGGSFPTDLSDISVGLYSQPRIEWILWQSLMNRKELARNVIYSDY
                     GVQHPDFNRLSTRFPSVSASVRYSGDNDFWVFRGRVANRFGYEQYGKHSEDILAHREY
                     SGPTFCAGDRDIEYYANEYQAYKANPSGNYKFGSPEVWRRIGQNHHITKVVEQLATLY
                     GL"
BASE COUNT          495 a          308 c          325 g          458 t
ORIGIN      
        1 atggatggtt tattggttca atacatgaca ccgaagatgg cccggtttgt gtatggctga
       61 gaaataaggc cgaagcataa aggctataaa ccgactaaca actaaatact gaagatttaa
      121 atcagaaacg atttttatta aacccttaac cggagggatt ctgcaccctc agaacatcag
      181 gaggccgtct gaaagggcgg aacagataat gcttacgttg aaacatttta tcgacatacc
      241 aacatggtta gccgtcattg cttttgttaa aatacacatc cacttttctg tgcaatgttt
      301 aaccactggt catatcaaat ggcattcatg cgaaccatga tatagaatca tggcttgaga
      361 gagtcgatga aagcgcaact atggtatgag agacattgat gtaagaaagg ctgtgcatgc
      421 caagattctg agagatcatc ataaagatcc tgacacccta atcattgatg agtttacgat
      481 gaatctaggg gctagcagag ctgatatagc agtgatcaat gggcttatac atggttatga
      541 gttgaagagc aagagtgata acttgctcag attaccagcg caggtgcaac attactcatc
      601 agtgatggat aaagtaactt tggttgtctc tgattgccat ctttatgatg ctttaagcat
      661 agttccatca tggtggggga taaagcaagt tacgcaaggt gcacggcaag gtatccattt
      721 aaaaacaatt cgaactagca agttgaatcc acaagtggac aaactttcct taacaatgct
      781 tctttggaaa gatgaattgc tttccctatt aagtgatgta ggggagctac agaatttgaa
      841 aaataaacct aaacgcgtct tatggtcaaa actcgccaat agtatggatg ttggcgagct
      901 tcgtgaagct gttcgagtta aacttaaagc ccgtaaagag tggcgagttg ctcaacaacc
      961 ttagttatgt gatggttttg cccaatccta cgccatacct ctgggctacc aaatttatag
     1021 ttaccagagg gattggcttt gtaggcttga tactcgtttg cataatattc tatgtctcta
     1081 tctcccgcac agaatgtagg ccctgaatat tctcgatgag caagaatatc ctcactatgt
     1141 ttaccatatt gttcataacc aaagcgatta gctactcttc ctcgaaatac ccaaaagtca
     1201 ttatctccag agtatctgac gctggcagat acgctaggga atcgcgtcga aagcctatta
     1261 aagtcggggt gctgtactcc ataatcacta taaatcacat ttctggcaag ttcttttcta
     1321 ttcattaaac tctgccataa aatccactcg attcgaggtt gagaatatag accaacagaa
     1381 atatcactga gatctgtagg aaatgaaccc ccagaaagaa tcacttttct gtattcattg
     1441 aggtgcgcca gattgtttat caatcccatt gccaaagtat atagttcgcc ggaattaatt
     1501 ttatcctcgg ttaactcatc tcttaagtca ataattatat caatatttga taaaggaatt
     1561 cccagatgat taatgtaatg cgttat
//
LOCUS       DAZUFB010000311         1568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZUFB010000311 DAZUFB010000000
VERSION     DAZUFB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.320"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1568)
                     /locus_tag="ACHY3M_005387"
     CDS             complement(<1..>1568)
                     /locus_tag="ACHY3M_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4320063.1"
                     /translation="TVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVVADDGSERLV
                     STARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGY
                     FQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHD
                     YYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAP
                     DLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKD
                     GRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFL
                     KRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKM
                     PANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKH
                     EREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLV
                     NNWYPTASISDLLVV"
BASE COUNT          340 a          469 c          367 g          392 t
ORIGIN      
        1 acaaccagca aatcgctgat acttgctgtg ggataccagt tattcaccag ccatgctgat
       61 accgaaaact ccagcgtcat gtgaccgtga ccggcaggca tatcaataac gccactgtaa
      121 atcagcgtat tatccagcgc ggtacggtta taaatttcag caccgttttt ccgcactatc
      181 agacggcatg aggagtaaat atcagtatgc tctctctcat gcttagcgcc actgaatgcc
      241 accgccggaa taacaatctg ccggtcaaac ggctgatcgt cataaaccct gacggtaatg
      301 gtccctgatg gccaccgctc cggtgcccgg gagtcccgcg gaaaagcctt acccactgtt
      361 ttgactatat cgccttcaat ctggttggct ggcattttcc ccttaatctg acagttctca
      421 ttaattgtga cattgttgag cgtcccggcg ttcgcattca cactgccact gatatctgca
      481 tttttagcgg tcagctttcc gtccggtgtc agggaaaatg ccggaggatt accgccgctg
      541 gtaatggtgg gagccgtcag gcgtttcagg aacacgtcgt tcatgaatat ctgatcgccc
      601 tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct
      661 gccgccagca gcacctgact ctgcattcct gctggcgtat tctcaatacc ggcaccgata
      721 cccgcaatat aaaggcgtcc gtccttcatc tgttgcagct tcacagccca catgctgttc
      781 agattattat ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc actctggtct
      841 tccagtttct tattgacggt ctgcgtgatt tcattactga catccgtgat ggacgttctg
      901 atttccgcca ggtcaggcgc aagctgaccg ttatcaatct gagtccacaa ctcctgagcc
      961 agatgggttt tccctatctc tcctttgaaa aaatccagat agccggatgc atcatcactc
     1021 ggctggccaa cagcctccac aaatgccgat ttgccaacgg tgttcacact gcggatataa
     1081 aagtaataat catggcccgg tttgatattg atactggcgg ctatccagta cagccccgtg
     1141 ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg taatccgcgt ttccgaaaac
     1201 cagaactcaa actgtaccgt cgggtcatac accgcaagac gcgggaccgc tgttatctga
     1261 aaatagcccg gtgtcagctc aatctgtgac ggcgctgccg gtgcggcaat ccggaacgat
     1321 accgacgccg gatcgccctg ctgcccccac gcatttaccg cccggactgt cagcctgtag
     1381 ttccccggcg ccagttgcgt gaagcggtat gtggtttccg tcgtccgggc cgtgctgacc
     1441 agccgctcac tgccgtcatc cgcgaccacg gtcaggcgaa gcataaagct cacgcccttc
     1501 accaccttcg gcgtgtccca tttcgcctgc gccagatact gaccgtcagc tgcgctcacc
     1561 tccaccgt
//
LOCUS       DAZUFB010000312         1568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZUFB010000312 DAZUFB010000000
VERSION     DAZUFB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.321"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(151..804)
                     /gene="ribB"
                     /locus_tag="ACHY3M_005388"
     CDS             complement(151..804)
                     /gene="ribB"
                     /locus_tag="ACHY3M_005388"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFS4320064.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(908..1056)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1178..1468
                     /gene="ubiK"
                     /locus_tag="ACHY3M_005389"
     CDS             1178..1468
                     /gene="ubiK"
                     /locus_tag="ACHY3M_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFS4320065.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          446 a          404 c          348 g          370 t
ORIGIN      
        1 tgatcataat gaaaaagaat gcctgatatt aatgaaattt tctcatagaa agagaagcgg
       61 aattaacatc ttgcatagca gggcaaaata aaaaccgaag ccagctacaa cttcggtttc
      121 ttgattaagg cagtaattaa gcagcggttt tcagctggct ttacgctcat gtgcctgacg
      181 gtatgccacc aggtcttcaa tagtcacgag cgccatattg tgtttattgg caaactcaat
      241 gcactctggt gcacgcgcca tcgtgccatc gtcattagtc aactcacaca gcacaccagc
      301 cggtttaaag cctgccagcg tcatcaaatc aatagtcgct tcagtatgac cgccacgcgt
      361 cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg cccgggcgat tcagatcaga
      421 aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta attcgatcag cggcagaaac
      481 accggtagtc acaccttcag ccgcttcaat ggtcacggta aaaccggtgc cataggcgct
      541 ggtgttattt tctaccatca ttggcagatc aagttgttta cggcgatctt cagtgatgca
      601 caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc atctgctcaa cggtcatggt
      661 ttctgccggg aagatcatat cgccttcgtt ttcacggtct tcatcatcaa gcaccattac
      721 accgcgtcct tcacgcagcg cagccagtgc attttcaaca cgttcgaaag gcgtaccaaa
      781 agaggaaagt agcgtctgat tcatggtaaa aaaacctcac taaaattatg gttaccagaa
      841 tcagggcagt cttaggagtg gcggcatata gccaaaataa cgtgagcggg tccatgcccg
      901 acagaatcgt tactctctcc catccggact ctaaccgtcg gccccggaat tacaccggat
      961 ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct ttcaactgag ttgatttacc
     1021 gccggtgggg aatttcgccc cgccctgaga ataagcggat tcactataac gctaatgatt
     1081 agcggcagca acgcatagct tcacataatt ctggtttatg acttaccctt atcgcactac
     1141 aatggcactc aacacctatc agtacaggga aactacaatg attgacccga aaaaaattga
     1201 gcaaatcgct cgccaggttc acgaatcaat gcctaaagga atcagggagt tcggggaaga
     1261 tgtggagaaa aaaatccgcc aaaccctaca agctcagctg acgcgcctcg accttgtaag
     1321 ccgtgaagag ttcgacgtcc aaacgcaagt cctgttacgg actcgtgaga aactggcgct
     1381 gcttgaacaa agaatgagcg aactggaaaa tcgcagtact gagattaaaa agcagccaga
     1441 tccagagact ctccccccaa cgctgtaaac atttgtcttt aatgcactgt aaattatgaa
     1501 ttaatcctct aatttacagt gctttctatt accttatcgc ttaattccat tcagaatatc
     1561 atcacaaa
//
LOCUS       DAZUFB010000313         1556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZUFB010000313 DAZUFB010000000
VERSION     DAZUFB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.322"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            842..1444
                     /gene="fimB"
                     /locus_tag="ACHY3M_005390"
     CDS             842..1444
                     /gene="fimB"
                     /locus_tag="ACHY3M_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFS4320066.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKSWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          467 a          265 c          340 g          484 t
ORIGIN      
        1 aagtataaac aaggaaagat ttgcaagcta actcagtgag cgtggtgaaa atcagtgttt
       61 acccgccatc aggctgagca taattctcat catgaaatat gtttcctggt ttgtggcttg
      121 taactggtca cttctgaagt cgatctggag aggcttgttg atgttggtgt tttcaggatg
      181 atgtttcact tagtttgttt gccgtatcgc ccggcgaatg gctgtgattg aggaaggttt
      241 aagtcgtagt gaccaaagct atatttacca acgaatgtag atgaaaaaat catctcctgc
      301 gttccccata tctccaggat aaaaaggaat gtaacaatct catggcgtaa gctgacgaat
      361 cagccaggaa taatcgctag ggacctaaga attagcatga taatagccac taagaaatta
      421 ctgcgctcca tgaaatagcc attttgtggc aaatggagtt gactaataat gtcatatgtg
      481 agacggctag ttgaacgaat attaaatttt gctgaatttt ttatgttgat tttacttgtt
      541 acagaacata tcacatgata tatagataag attagttgca ttaatgatga gggttattat
      601 tagattcgta tccgattgat aaatatataa aggtacatag catgcaagag catggcgttt
      661 gtatggcaac gttattataa ttaactgttg ctactccatt taagttcact cagaagaact
      721 ggtccactta cgttagttat taagcaaacg ttcgctttta taaacataat caggataaaa
      781 atgttggatt attgctaacc cagcacagct agtgcgcgtc tgtaattata agggaaaaac
      841 gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg aaattgaatc
      901 actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct
      961 ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga
     1021 tcttaaggca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgcatcc
     1081 gctattgaac aaagaagttc aggctttaaa aagctggttg agtatccgta cttcgtaccc
     1141 gcatgctgag agcgagtggg tatttttatc tcgtaaggga aatccgcttt ctcggcaaca
     1201 gttttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc
     1261 gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact
     1321 tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa
     1381 tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc acgctgtttt
     1441 atagagatag ttgagatacc agggatggtg ttaaaaacta cattattctt acgatatatc
     1501 aaaaatgatt aaccatttat taattgatgt tattgtaagt gtgattatta attttc
//
LOCUS       DAZUFB010000314         1551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZUFB010000314 DAZUFB010000000
VERSION     DAZUFB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.323"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            312..638
                     /locus_tag="ACHY3M_005391"
     CDS             312..638
                     /locus_tag="ACHY3M_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFS4320067.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(770..970)
                     /locus_tag="ACHY3M_005392"
     CDS             complement(770..970)
                     /locus_tag="ACHY3M_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4320068.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            1012..1377
                     /locus_tag="ACHY3M_005393"
     CDS             1012..1377
                     /locus_tag="ACHY3M_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4320069.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          365 a          331 c          425 g          430 t
ORIGIN      
        1 gtattccctc attaacagag accgcagccc gacagggaga ctcctctgcg cgagtgtgcg
       61 gggagaatca aaaacgatac gcaccggggt ttaccgcgtt aacggagcgc ggcgttgtcc
      121 cctcatggtc gctggttcgg tgcgatggtg gaagaaaccg gacgatgtgt tacctcgcaa
      181 gacctgttat gtcatgtgtc tgatttgtga tttaagtcgg ataattgtcg ttgccattaa
      241 gcagaggatt gatggccgac agggtggcat tgttagaata agacttattc ttatctgtgc
      301 cgggaatgaa aatgaaaaga aatcttccgt taattatttt gttgtcttct ctggttatgg
      361 gctgtacgca acataaaaca gatatgcccc gacagttggt taaggcatta ccacaatatc
      421 cggcctatgc agcggcaaat tatataaagg gacgggttga tgtgaagttt gatattggtg
      481 ctgatggtac tgtcacccga attgagttta tccgttcaga gccgcaccat ctgtttgatg
      541 agcaggttgt aaaggcgatg gcaaaatggc gatttgagaa ggacaggccg cgtaaaggcg
      601 tgaagaaaac gtttatcttt agtccttctg caccctgatt atttcatcag aaattaatta
      661 tcactctgtt gttattctgt acattccggc tgggtaagtc ttgttccgcc gggtatgaag
      721 atgaaatatt gttggaggac agtgggtacc tgctcctgta accgaacgtt catttctcgt
      781 tatttgtcat gctggccgga cgcagatgcg ttgcatctgt tgccagcctt ctcctgcagg
      841 cttcaataac ccacgctgaa aagttaccgg aacctttatg ttcaagggcg atattgatct
      901 gttcaatcat gtgattgggg aaacggatat tgcgggttgt ggttctgcgg gtccggtttt
      961 tcgatgacat atttatttcc tttactgatt gccatatgac ggggatttta catggctgag
     1021 cttcgtacac tccagagcag aatcaaaaca ctgaataccc gacgggtgaa tattctgaag
     1081 ggtgaacagc gtcgtgtcag tggcagtgca cgtgtttccc tcaagcgtca tatctggctc
     1141 agggatgccg ggcagtgctg tctctgtggt cgtgtggttg acctctgtga cagtgaactc
     1201 gatcaccgaa ttgcacttca gttcggtggt ggtaatgagg agacgaatct ctggacgctc
     1261 tgtaccgaat gccatcgaca aaagtctgct cgtgaagcgg cgggtggtat gccggacccg
     1321 acgctgccgg aggtgtccgg aggtagtggc agagcggacg acatcatcgg actgtaaccc
     1381 gagcgggggg gtatcatccg gcgtaaaaaa cgatcgctcc ggacaccgcg ccccctctca
     1441 cgcagagaaa aaattcccgt ttcagggcag ttaacatgtt aactggctgt ccgggcattt
     1501 ttgcggtttt tatctttatt attcagtttg ttgtgcgaaa aaaatgttaa c
//
LOCUS       DAZUFB010000315         1548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZUFB010000315 DAZUFB010000000
VERSION     DAZUFB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.324"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..764
                     /locus_tag="ACHY3M_005394"
     CDS             <1..764
                     /locus_tag="ACHY3M_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320070.1"
                     /translation="RDIPILTFEAPDDFISRAAWLRESEVLTWCEEYLKPLLEALNPR
                     SRFSFGEDFARIGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQVMMYILTRVPA
                     LVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKAEFEDQNILVAR
                     HQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCMANRASYMEGFI
                     LDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            764..>1548
                     /locus_tag="ACHY3M_005395"
     CDS             764..>1548
                     /locus_tag="ACHY3M_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFS4320071.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRT"
BASE COUNT          315 a          419 c          474 g          340 t
ORIGIN      
        1 tccgcgacat cccgattctg acgtttgaag ccccggacga cttcatcagc cgtgccgcat
       61 ggctgcgtga atcggaggtt ttaacctggt gtgaagaata tttaaaaccg cttttagagg
      121 cgttaaatcc ccgttcccgc ttcagcttcg gggaagactt tgcccgcatc ggtgacctct
      181 cctgctttgt gctgctggaa atcactgaat ccctggcaaa acgcgaggtg tttcgcgtgg
      241 agctgcgcaa cctgccgtat gcccagcagg agcaggtgat gatgtacatc ctgacccgcg
      301 ttccggcgct ggtcggtgcg gcgttcgacg ccaccggtaa cggcggctat ctggccgaag
      361 ccgcgctgct ggcctttggc ccggacatca tcgactgcgt gatgttatcg ccgaagtggt
      421 acggcgagtg gatgccaaaa ctgaaggccg agtttgagga tcagaatatc ctcgtcgccc
      481 gccatcagac cacgcttgat gatttgcgcc atgtgaaggt ggtgaacggt atcccgcaaa
      541 ttgacaaggg gcgtacgaag gaccagaacg caacggctgt gaacgcccgc cgccatggcg
      601 attttgccgt ggcgctctgt atggcaaacc gggcgtcata catggagggc ttcatcctgg
      661 atgaatcagc ctgtcaggcg ctgccggagc ggtcgcgggc catggagggc ggttatcgtg
      721 atgacgatga ggcatatcat gaatttgatc gggggtgctg gtagtgggac gtataattga
      781 tcttgacgga aaacctttct cctttgaccc ggagatgcag agcgccgcgc tggatattcc
      841 gcagattgcc agccgttata ttgaacatcc ggcctcgggt atcaccccga accgggcggc
      901 acagtgcctt cgcggggctg aacgtggcga tctgattgcc cagtccgatc tggccgctga
      961 cattgaagaa aaggataccc acctttttgc agagctgggc aagcgacgtc ttgccattca
     1021 gggcgtgccc tggagtattg agccgccccc gaacgccagc gcgaatgaga aaaaggacgc
     1081 ggaaatgctc gacgaatatc tgcattccgc cgactggttt gatgccatgc tgtttgatgc
     1141 caccgatgcc atcctgaaag gctattcctg catggagatt gagcacggga tgctcggtaa
     1201 aatgcacatc atccgcgcca tccgctggcg tgacagcggg catttctgcc ttaacccgga
     1261 tgatttgagc gaactgcggc tgcgtgacgg cagccatgcc ggggtggcgt ttcagccctt
     1321 tggctggata gtgcatcagt cacgttcacg caccggctac ggtggtgcga cggggcttgt
     1381 cagaacgctt atctggccgt tcattttcaa aaactattcc gtgcgcgatc tggctgaatt
     1441 tctggaggtg tacggcctgc cgatgaaggt cggtaaatac ccctccgggg caacaccgga
     1501 gcagaaaagc gccctgatgc gggcggtgat ggatatcggt cgacgtac
//
LOCUS       DAZUFB010000316         1542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZUFB010000316 DAZUFB010000000
VERSION     DAZUFB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.325"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1103)
                     /locus_tag="ACHY3M_005396"
     CDS             complement(<1..1103)
                     /locus_tag="ACHY3M_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFS4320072.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGAL"
     gene            complement(1153..1455)
                     /locus_tag="ACHY3M_005397"
                     /pseudo
     CDS             complement(1153..1455)
                     /locus_tag="ACHY3M_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          317 a          505 c          362 g          358 t
ORIGIN      
        1 ctgagtgccc ccagtgtatg atcacctatc catgcccatg cgtcagaagt ccaggtttta
       61 acatcgtccc agattttttc caccggcgtg gccgctttat caagttgctc cagacgtgca
      121 ttcatgacat ccgcaaacag ggacatcgcc tccgtcaccg cagcctgttt acctttcgtg
      181 cgctcaagct catcaatatg gcgtaactgg gaaacgctca ggaagttata ctgctgattc
      241 agggaggcca gcgccttcac cggatctgct gcaatccctt caaaggcttt ttccaccttc
      301 ccggcatcgt cccccaccgt ctgcagccat ctctgagagg tttcccccat gatccgtagc
      361 tgcccggcgg tatatttccc gctttctgcc agacgggcca gattttctgc cgcctgtctg
      421 ataccaccac cggcttcatc gctgatcacc ccggccattt tccacaattc tgccgttgtg
      481 gtggcagccg cccctccggt caggatcagt gaacgcaata aggcccgatc agcctgctct
      541 gcctgccagg cggcggcagc aagcgcggcc agtacggcaa ccccgccacc tgccgccaca
      601 cgggccaccg acataaatcg tcccagctca ccggcattcc gggcattttc agccagtgca
      661 tttgccgtat ctgacagcga ctcctctgat gattcagagg catccctgat cccgagaagt
      721 tcctccttca gcagggtaag caggctgagc ggtccaccga atgaatcgct gatctgcccc
      781 ccctgctgca gcatgataag gaagggattc tgaccaccgg caagctgagt gacaatatcc
      841 gtgaactgtg cgggcagtgt gcgcatggca gccttatact gtccgactga tatcccggct
      901 ttttgtgcag ccagcgcctg tcggctcaga ctctgctcag cagcatcagc ctgttttctg
      961 aaagactgac tgactttccc ggacatcaaa tcagcaagcg cactggtttc acccagctct
     1021 ttttttaccc gcgctgcctc ttcagaaaaa cgggcagaat ccagtgtcag cacggctgtc
     1081 agatcggcaa aattacctgc catcgtggtc acctcctgaa atgtcctctg ataccatcaa
     1141 taactgtcac aacctccttc cctcctcccc gaaacggact ccaccggcga ggcccgccgc
     1201 cttctgcatc agtacatcat tttcgtccgg cgtctccgcc tgtccttcct ctgcctccgg
     1261 agcgaacagg ctgaaatccg ccggatgcat atccggatcg caaaaaaaca ggctgagtac
     1321 ggcgtacatc agcccggaaa aatgcatatc cagctgggta tcctgaaaat aatgcgtgca
     1381 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
     1441 gtcggctctc cccatctcac gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
     1501 ttttttgatg ttaccggctc agtcgcttct gttttcccgg tt
//
LOCUS       DAZUFB010000317         1539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZUFB010000317 DAZUFB010000000
VERSION     DAZUFB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.326"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..1536
                     /gene="ygeH"
                     /locus_tag="ACHY3M_005398"
     CDS             160..1536
                     /gene="ygeH"
                     /locus_tag="ACHY3M_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFS4320073.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFPPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYNETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          555 a          267 c          263 g          454 t
ORIGIN      
        1 agattacatc actgaattcc taagataaat tagtgaacac aaccatagat atgtcgaatg
       61 taatttcctg tagcgttaat gacacataaa tatgaatagc cataatttct attgcttaag
      121 caggatgcaa gaaaccaatt ttttcataga ggttaactaa tggacttaga aaataaattc
      181 tcatatcatt ttcttgaggg attaacgctc acggaagatg gaattcttac tcaaggaaat
      241 gagcaagttt atattccaca gaaagagtta ggtgtattaa tagtattact tgaatccgct
      301 ggtcatgttg tactgaaaga tatgatcatc gaatcagtat ggaaaaatat tattgttagt
      361 gacgagtccc tgacaagatg tatctattct ttgcgctgca tttttgaaaa aattggctat
      421 gatcgttgca tagaaacaat ctaccggaaa ggttatcgtt tcagcgggca ggttttcaaa
      481 actaaaatta atgaagataa tacttcagac tattccatag ctatattccc tttcactact
      541 tcgttgaata cactggatcc attaatactt aatcaggaat tagtgcaaat catttcaaat
      601 aaaaaaatcg atggtctcta tacctatccg atggctgcga caaatttttg taatgatcac
      661 atatctcaaa attcattctt gagcagattc aagccagatt atttcgttac aggaagaata
      721 aaccagaata atgcagtgaa cactttatac attgagttga tcgacgctaa aaaccttttc
      781 ctcatcgcca gtaatcatct ccctgttgat gaactacata atacatcaca atttattata
      841 gataatatcc ttcaaacggt acataaacca gaacgatctg taagattagc taagcaggac
      901 caaggatata agaatcatta tttatcagat gaaatgttag ccggaaagaa agaactttac
      961 gacttccccc ctgaaagcat ttacagggcc atgactatat ttgatagatt acaaaataaa
     1021 agtgatatac agacgctaaa aacagaatgt tattgccttc tagcggaatg ccatatgtct
     1081 ttggcacttc atggaaaaag tgaacttgaa cttgctgctc aaaaagcatt agagctttta
     1141 gattatgtat cagacataac cactgtcgat ggaaaaattt tagctattat gggactgata
     1201 actggtctgt ctggacaagc aaaagtatct catatcttat ttgaacaggc taagatacac
     1261 ccaactgata tagcctctct ctactactat agggcactag tccactttca taatgaaaaa
     1321 attgaagagg caaggatttg tatagacaaa tcactacaac tcgaacccag aagacgaaaa
     1381 gcagttgtga taaaagaatg tgtagatatg tatgtgccta acccgctcaa aaacaacatt
     1441 aaactctact ataatgaaac tgagagtgaa agccatcgag ttataattga caacattttg
     1501 aaattaaagc agctgacgag aatttgtatg cgataaatt
//
LOCUS       DAZUFB010000318         1531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZUFB010000318 DAZUFB010000000
VERSION     DAZUFB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.327"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..752)
                     /locus_tag="ACHY3M_005399"
     CDS             complement(<1..752)
                     /locus_tag="ACHY3M_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS4320074.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
     gene            760..1185
                     /gene="tnpA"
                     /locus_tag="ACHY3M_005400"
                     /pseudo
     CDS             760..1185
                     /gene="tnpA"
                     /locus_tag="ACHY3M_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          402 a          370 c          337 g          422 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcttcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga
      241 gttgatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctgttcc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 acatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttctg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgacgg ggcatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccgac ctcatgattc tcattctgac gtgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacgct cctgttgacc accgggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggccaaa tgatactttg gtctgtgaaa
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gataccgacg tcagattttt gaccatgacg cgacagaaaa actacgcact
      901 tacttttcaa atgtatgtgc tgattttgaa gctgaactgg ttgaaatgga tggcgaacca
      961 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac
     1021 agcctcaaag gcgtatcggg taggtgactg cgacgagatc gaccagatat tgcagtcagg
     1081 tattactaca aaggcgtttt gtggagtgct ggctattttg ccagtagctg cggaggtgcg
     1141 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtca ggtggaaaac
     1201 cgcgccttat atccctgccc tgaaggacgg ggttttacgg cgcaccggat aagcctggag
     1261 attatctaca ccagcatgga catcattgta ctatgccccg tacatgcccc atcacatcct
     1321 caacatcaaa gtattagagc cttgcaaacc attatgcgtc tcagtttgtc tcatctatct
     1381 tacgcaaaaa aaatagaact aatatcaagc ggcgctatat tgcgtattaa ataaaaccac
     1441 ctcaattaca ttaataaatc caaacaaaca tcatgcaaat aattcccatg gttatttcta
     1501 ggctatttaa attaaataaa taaattatga g
//
LOCUS       DAZUFB010000319         1519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZUFB010000319 DAZUFB010000000
VERSION     DAZUFB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.328"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..>1519
                     /locus_tag="ACHY3M_005401"
     CDS             156..>1519
                     /locus_tag="ACHY3M_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFS4320075.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVA"
BASE COUNT          422 a          288 c          401 g          408 t
ORIGIN      
        1 atcaattcga aagtattagc aaattagcca aattgacacc ttcccacctc caactaacac
       61 tcaatacaga cctctgacaa tttaataagt ttcattttta atgtctgacg actcatctct
      121 ttcgtggctg tattgtttgt atatggagcg tttatatgaa tagaatctat cgcgtgatat
      181 ggaattgcac tctacaggta tttcaggcct gctcggaatt aactcgcagg gcaggtaaaa
      241 catcgacggt taatttgcgt aaatcctctg gactgacaac gaaattcagt agattgacgc
      301 tgggtgtttt gctggcacta agcggttcag cgtctggtgc aagtctggaa gttgataatg
      361 atcagattac caatattgat actgatgttg cttatgatgc ctacctggtt ggctggtatg
      421 gcactggagt gcttaatatt ttggctggcg gtaatgcctc cttaaccact attactacca
      481 gcgtcattgg cgctaatgag gactcagagg gcaccgttaa tgttttgggt ggcacctggc
      541 gattgtatga tagcggaaat aatgcaaggc ctttaaatgt gggtcaatcc ggaacgggga
      601 cgctgaatat taaacagaag ggtcacgtcg atggaggcta tttaagatta ggttcttcga
      661 caggaggcgt cgggacggtc aatgttgagg gagaggactc tgttctgacg accgaattat
      721 tcgaaatagg gagttacggt acgggctcat taaatattac ggataagggt tacgtcacga
      781 gttcaatcgt cgccatttta ggctatcagg cgggcagtaa tggccaggtt gtcgttgaaa
      841 agggtggcga gtggctaata aaaaataacg attcctcaat tgaatttcaa attggtaatc
      901 aaggaactgg ggaggcgact attcgcgagg gtggactgat tacggctgaa aatacgatta
      961 tcggtggcaa tgccaccggt gtcggaaccc tgaatgtgca ggatcaagac tctgtcatca
     1021 cggtacgcag actctataat ggatatttcg gtaatggcac actcaatatt tccaataatg
     1081 gactaattaa taacaaagaa tattcattag tgggcgttca ggacggttcc cacggtgtcg
     1141 tcaacgtgac cgataaaggg cattggaatt tcctcggaac gggcgaagct ttccgctata
     1201 tctatatcgg tgatgctggc gacggtgaac ttaatgtctc gcgtgaaggt aaagtagatt
     1261 cgggaattat cactgcgggg atgaaagaaa caggcacagg caacattact gttaaggata
     1321 agaactccgt tatcactaat ctcggaacta atcttggtta tgacggccac ggcgaaatga
     1381 atatcagtaa tgaagggctt gttgtcagca acggaggaag ctcactcggt tatggagaaa
     1441 ccggcgtcgg gaatgtcagc atcaccacgg gggggatgtg ggaggtcaat aagaatgtct
     1501 ataccaccat tggtgttgc
//
LOCUS       DAZUFB010000320         1471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZUFB010000320 DAZUFB010000000
VERSION     DAZUFB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.329"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..221)
                     /locus_tag="ACHY3M_005402"
     CDS             complement(<1..221)
                     /locus_tag="ACHY3M_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFS4320076.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GMSFFVDIVGLVNTTIDSLNRPFLYAIRE"
     gene            complement(214..990)
                     /gene="escT"
                     /locus_tag="ACHY3M_005403"
     CDS             complement(214..990)
                     /gene="escT"
                     /locus_tag="ACHY3M_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFS4320077.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            complement(990..1259)
                     /gene="escS"
                     /locus_tag="ACHY3M_005404"
     CDS             complement(990..1259)
                     /gene="escS"
                     /locus_tag="ACHY3M_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFS4320078.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(1259..>1471)
                     /gene="escR"
                     /locus_tag="ACHY3M_005405"
     CDS             complement(1259..>1471)
                     /gene="escR"
                     /locus_tag="ACHY3M_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFS4320079.1"
                     /translation="FTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          541 a          272 c          242 g          416 t
ORIGIN      
        1 atttctcgaa tggcatacaa aaacggtcta tttagcgagt ctattgtcgt attaactaac
       61 ccaactatat caacaaaaaa actcatgcca tacaaagaaa aaaatgaaaa taagattaat
      121 gactgcactg cagccattac ctcttcactt tttgttacat cgcccttctt ttttagatcc
      181 cttagttttt tgggtgtggg cttttctgtt ttttcactca ttaatcatgc tcggtaacga
      241 tctatcaatt atgtcagaat gaatatttgc cgtaataaaa tcaggaaaaa catgaataac
      301 taatagcaat ataaagattg ctatcaaact ttttactggc agtgataatg tgaatacgtt
      361 tagctgaggt gctgttttgt taagaaaccc aaaccccata tcacataaga atataccgat
      421 aatcatggga actgaaaatg ataacatcag tttaataaat gaatcccata gagaaaacaa
      481 aaaatcaatt aaagcacgat tgaatgcaat atcggcttgt aatggtaata tctcgtagga
      541 taaatatagc ttatctagga tgctttgtat cccaccatga ataacaaaga tcacagagat
      601 aaattggtac aaaataacgc cagtgataga agatgaatca cttatggacg ggttaaaaat
      661 tgaagatatt gttgaacctc ttagagtatc aataatctgc ccagccgcat ctattgccca
      721 aaaaagaata gtaaatgata acccaatgaa aaaaccaata cttatttctt ttagcgctat
      781 tcctgtcaac tgaaagattc cagatggcaa tttttccgaa aatgtatagt tctctactat
      841 gatcggaagc gtaaacgcaa tcataattga gttgcgaata aagttactga gtaaaacacc
      901 tgttgaaaat attggtagga taataaacat ccccagcggc ctgagtatac aataaaatga
      961 tgatactatg accgtcatta tctcattcat tagccgttca ccttcggaat catttcaaat
     1021 attatcgacg agaagttgat gattgttgtt cccatccagt gataagtcag ggcaagcgta
     1081 gcaaacactg ctattatttt tagcaaaaaa ggcaatgttt gatcctgtaa ctgcgttata
     1141 gcctggacca gactaataat aataccgata acagaagccg ctatgactgt aggcaatgag
     1201 aggataaata ttatccagaa cgtttgcaca cataattgaa caaaatatcc agtatccatc
     1261 aattcaccac caacagaaat tcgaataatt tttgccagcc tccaacaaga atgaataaca
     1321 agatcttaaa aggaatcgaa attgttactg gcgataccat catcataccc aaggccaata
     1381 agatattgga aatgatcaaa tctatcgcaa taaagggtaa atagagcaaa aaacctatct
     1441 tgaatgcagc ttcaagctgc cccatcgtga a
//
LOCUS       DAZUFB010000321         1457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZUFB010000321 DAZUFB010000000
VERSION     DAZUFB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.331"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..>1457
                     /gene="nleA"
                     /locus_tag="ACHY3M_005406"
     CDS             143..>1457
                     /gene="nleA"
                     /locus_tag="ACHY3M_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFS4320080.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTSTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFDIIQET"
BASE COUNT          469 a          312 c          301 g          375 t
ORIGIN      
        1 caacttaatt ttgagatcta agtcacatca atcaagcatt caaacagcta tatattaaag
       61 ctgtccacat cggatatgtg acactaataa tatcaatgga ttgatattat taatggatat
      121 aaacatgcaa taaggattta tcatgaacat tcaaccgatc gtaacatccg gaatcaccac
      181 acaaaacaat cgacatcatc acgcagaaca aacgtcccct acacaaatac cgcaatccga
      241 attacctaat ggatgcgaaa cgggatttgt tgttcatatc ccagaggata tgcagcgaca
      301 tgcaccggaa tgcggtgaaa caacagctct actgagcttg ataaaagatg aaggtctgct
      361 ctctgggctg gataaatatc ttgcacctca tcttgaagaa ggctctgcag gaaaaaaagc
      421 attggatatg tttggtttat tcaatgtctc tcagatggca ttagaaatac ccagcaccgt
      481 tccgggtatc tctggtaaat atggtgtcca gctaaacatt gtaaaaccag atattcatcc
      541 tacatcaggt aattattttt tacagatatt ccctttgcat gatgaaatag gtattaattt
      601 taaagacctt cctggtccat taaaaaatgc attaagcaac agcaatatac caaccactgt
      661 atcgactgct gcatccacta ttgcatcagc cactacttcg acggtaacca ccgcgtcaaa
      721 agacccaata ccatggtttg gattaacagc tcaagtagtt cgtaatcatg gtgtggaact
      781 tcctatagtc aaaactgaaa atggatggaa gcttgttgga gaaactcctc ttactcctga
      841 tggccccaaa gcaaattata ctgaagagtg ggtgatcaga ccgggagaag cagattttaa
      901 atatggtgca tctccactac aggcaactct agggctggag tttggcgcac atttcaagtg
      961 ggatttagat aaccctaata ctaaatatgc cgttcttacc aatgctgccg caaatgcgct
     1021 tggtgctgta gggggatttg cagtatccag atttactagt acagatccaa tgttaagtcc
     1081 tcatatcggt gcaatggttg ggcaagcagc ggggcatgcc atacagtata atacccccgg
     1141 attaaagcca gacactattt tatggtgggc aggtacgact ctggggctgg cagatttaaa
     1201 caaagccgag acaggtgaag gcagattcac tgactatcct cgtatatggt ggcacgcaag
     1261 agaaggtgcc atcttcccta ataaagaaga tattgcccgt gcaacagggg ctgatatacg
     1321 agctatggaa gaaggtatac ccgttggaca gcgacatcca aatccagagg atgtggtcat
     1381 cgatatcgaa agcaatggct caccacatca taacccatca aattatgttg atacctttga
     1441 tataatccaa gaaacaa
//
LOCUS       DAZUFB010000322         1450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZUFB010000322 DAZUFB010000000
VERSION     DAZUFB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.332"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1450
                     /gene="rhsD"
                     /locus_tag="ACHY3M_005407"
     CDS             <1..>1450
                     /gene="rhsD"
                     /locus_tag="ACHY3M_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFS4320081.1"
                     /translation="EMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVL
                     HTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDIT
                     TPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHS
                     ELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYR
                     HYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGL
                     TRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAY"
BASE COUNT          338 a          405 c          501 g          206 t
ORIGIN      
        1 ggagatgcgc taccgctacg acgatgcggg gcgggtggtg gagcaactta acccggcagg
       61 cctgagttac cgctaccagt atgagcagga tcgcatcacc gtcacggaca gcctgaaccg
      121 gcgtgaggtg ctgcatacag aaggcggggc cgggctgaag cgggtggtga aaaaagaact
      181 ggcggacggc agcgtcacgc acagcgggta tgacgcggca ggaaggctca cggcgcagac
      241 ggacgcagcg ggacggcgga cagagtacgg tctgaatgtg gtgtcaggcg atatcacgga
      301 catcaccacg ccggacgggc gggagacgaa attttactat aacgacggga accagctgac
      361 ggcggtggtg tccccggacg ggctggagag ccgccgggaa tatgatgaac cgggcaggct
      421 ggtatcggag acatcgcgca gcggggagac agtacgctac cgctacgatg acgcgcacag
      481 tgagttaccg gcgacgacaa cggatgcgac gggcagcacc cggcagatga cgtggagccg
      541 ctacgggcag ttgctggcgt tcactgactg ctcgggctac cagacccgct atgaatacga
      601 ccgcttcggc cagatgacgg cggttcaccg cgaggaaggt atcagccttt accgccacta
      661 tgacaaccgt ggccggttaa cctcggtgaa agacgcacag ggccgtgaaa cgcagtatga
      721 gtacaacgcc gcaggcgacc tgactgccgt tatcaccccg gacggcaacc ggagcgagac
      781 acagtacgat gcgtggggaa aggcggtcag caccacgcag ggcgggctga cgcgcagtat
      841 ggagtacgat gctgccggac gtgtcatcag tctgactaac gagaacggca gccacagcga
      901 cttcagctac gatgcgctgg accggttggt acagcagggc ggctttgacg ggcggacgca
      961 acgttatcat tatgacctga ccggaaaact cacgcagagt gaagatgagg ggcttgtcac
     1021 cctctggtac tacgatgaat cggaccgtat cactcaccgc acggtgaacg gcgaaccggc
     1081 agagcagtgg cagtatgatg accacggctg gctgacagac atcagccacc tgagcgaagg
     1141 ccaccgtgtc gctgtccact acggctacga cgataagggc cgcctgaccg gcgaacggca
     1201 gacggtggag aacccggaga cgggggaact gctgtggcag catgagacaa cacacgccta
     1261 caacgaacag gggctggcaa accgcgtcac gccggacagc ctgccgccgg tggagtggct
     1321 gacctacggc agcggttatc tggcgggcat gaagcttggc gacacgccgc tgctggagta
     1381 cacgcgcgac cgcatgcacc gggagacggt gcgcagcttt ggcagcatgg caggcagtaa
     1441 tgccgcatat
//
LOCUS       DAZUFB010000323         1436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZUFB010000323 DAZUFB010000000
VERSION     DAZUFB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.333"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..87
                     /locus_tag="ACHY3M_005408"
     CDS             <1..87
                     /locus_tag="ACHY3M_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320082.1"
                     /translation="EARKDGWRISKDKTRAFAPGHKVLRINK"
     gene            84..209
                     /locus_tag="ACHY3M_005409"
                     /pseudo
     CDS             84..209
                     /locus_tag="ACHY3M_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            619..807
                     /locus_tag="ACHY3M_005410"
     CDS             619..807
                     /locus_tag="ACHY3M_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320083.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            809..987
                     /locus_tag="ACHY3M_005411"
                     /pseudo
     CDS             809..987
                     /locus_tag="ACHY3M_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1321..>1436
                     /locus_tag="ACHY3M_005412"
     CDS             1321..>1436
                     /locus_tag="ACHY3M_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320084.1"
                     /translation="MITITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIA"
BASE COUNT          410 a          301 c          392 g          333 t
ORIGIN      
        1 gaggcccgta aagacgggtg gcgaataagt aaagacaaaa cacgtgcttt tgcgcccggg
       61 cataaagttt tgaggattaa caaatgacca ctattaccaa agaacgtatc gaattattca
      121 ttaaaaatcc gcttgataac gggcttaccc gtggcgaaca aatggaactg gcacgaattg
      181 cgctggcatc actggaagcc gaaccggttg cagtaaacga cgacatggct tacgcattcc
      241 atcacgcatt gtcagattca tcgctaggcg ctgatgaagt agaggaaatt aaggccggtt
      301 tgcgtgctgc ctttgccaac gtcactatcc aaccagagcc ggtagtgccg gatgaaatcg
      361 atccagacga tagcaatacg tttgattatg ttgatggctg gaacgcctgc cgcgctgcca
      421 tgcttcaggg taagggaggt gagtaatgcg tgtggcattt ttcggcttgt taccgtaccc
      481 gactcgtttt tgggcttctg cgctaattgc aaagccacat gtcctgatgg ctgacaacat
      541 catcccggca ccaaagcgcc gccataccgg tattgcagcg gcacgacgag cagcaaagag
      601 acgcaggaga gcaaagcgat gaaaaaccgt aaagcaaaga ttctgttagt tcgtagaaac
      661 gctcctggcg tctggcagtg ggtgagactc agcaaccgac ggatggggtt gatgaaatat
      721 tacgggatga tggattgtgg tttttgcaaa aagcccagcg cggcgcaaaa ccgctggaaa
      781 aaccacttgc gcactaaagg agagtgatat ggcgttaaca caccgcgaac tctgtcagat
      841 tgcgtataag ttccttaagc gcaacgggtt caaggtttgt tttcatgacc gctttatagc
      901 tgtaaccagt accggagaac agccagatgc tatgggattc agaaattcag catcatgcct
      961 gatagaggcg aagtgttctc gtgctgactt gttggcagat agaaaaaagc gttttcgtaa
     1021 aaatccatct cttggaatgg gcgactggcg attctttatt agtgagccgg gaattatttc
     1081 agttgaggat ttaccacctg gctggggatt acttcacgtt gttaacggaa gagtacggaa
     1141 agtacatggg tggcccaagg gtaattgctg ttggggtaat cctgacgata agccatttac
     1201 tggaaataag caggttgaat gcgattacat gttatctgca ttaaggcgca tggagttgag
     1261 agggcacctt aatgaaatat atgacggtgt aattgttaat aagaaagaag gaaacgcggc
     1321 atgatcacta ttaccaaaga gcgactgctg acaatcaagc agtggcgcga aacatacgga
     1381 ccgggtagca acgttgtact gccagcagaa gaagcggaag aactggcacg aattgc
//
LOCUS       DAZUFB010000324         1435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZUFB010000324 DAZUFB010000000
VERSION     DAZUFB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.334"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..576
                     /locus_tag="ACHY3M_005413"
     CDS             13..576
                     /locus_tag="ACHY3M_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4320085.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREVAAIPQPDENNPEMPPPAVIS
                     HGIEYMEDGLPDPVKAMGQILVENLIIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNQNEESG"
     gene            573..>1435
                     /locus_tag="ACHY3M_005414"
     CDS             573..>1435
                     /locus_tag="ACHY3M_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4320086.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAAWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEA"
BASE COUNT          351 a          366 c          435 g          283 t
ORIGIN      
        1 cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg
       61 aaaaacaaaa cagcgtcagc cattgccgcc ggttattcgg cgaagaccgc cagggttaaa
      121 ggctcgcagc tggcaaaaga tccggaggtg ctcgcgttta tagcccgtaa acagtgcgaa
      181 acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagca ggaggataaa
      241 ccccgtcgcc gtgaggtggc tgcaatacca cagccggacg aaaacaatcc ggagatgcct
      301 ccgcccgcgg tgatatctca tggtattgaa tatatggagg atggtcttcc cgatccggtg
      361 aaagctatgg ggcagatcct ggtggaaaac ctgataattg acccgaaact ggcactggat
      421 gcggcctggc gactggcgca gttcacacac cataaaaaag gcgatgccgg taaaaaatcg
      481 gcaaaaggtg atgccgcgaa aaaagcggct aaccgttttg cggtgccacc acctccccga
      541 ctggtggtga ataaccagaa tgaggaaagc ggatgatacc tgtatggagc acggcatgtc
      601 cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggatccg atttatccgg
      661 acgaggccgc acatgccctg gcgattttta aacaactgcg gattgtggat gcacctggta
      721 gcccgacatt cggggagtcc tgtgcagcgt gggtgtttga cctggtggcg gccctgtttg
      781 gctcctatga tgcgcagacc ggtgtacgcc atatcaagga agtgtttatc cttatcccca
      841 agaaaaactc gaagtccacg ctggccgcgg ggatcatgat gacggcgctg ttactgaact
      901 ggcggcaggc ggcgggttac acgattctgg ccccgactgt ggaggtggcg gccaacgcct
      961 tcaaccctgc ccgggatatg gtacgacgtg acgatgatct ggatgacctc tgccaggtac
     1021 agacccatat ccggaccatc acccacaggg tgacagacac caccctgaag gtggtggcag
     1081 ccgatccgaa tacggtgtcc ggtatcaagt ccgtggggac actgattgat gaactgtggc
     1141 tgtttggcaa gcagtacaaa gcggaagaca tgctacgtga agccatcggc gggcttgcct
     1201 cccgtccgga aggatttgtg gtgtacacaa ccacccagtc gaatgaaccg cctgccgggg
     1261 tgttcagaca gaaactgcag tacgcccggg atgtccgtga cggcaaaatt catgatccgc
     1321 actttctgcc ggtgatattt gaacaccctc ctgaaatggt ggaaagcggg gctcacctgc
     1381 tgatggaaaa cctcgccatg gtcaatccga atctcggtta ttcggtggat gaggc
//
LOCUS       DAZUFB010000325         1425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZUFB010000325 DAZUFB010000000
VERSION     DAZUFB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.335"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1425
                     /locus_tag="ACHY3M_005415"
     CDS             <1..>1425
                     /locus_tag="ACHY3M_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFS4320087.1"
                     /translation="AFNKAQNAAERLLLLAPGKRQEIKAEDINWLPEFLAQYRQSNGR
                     PMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQKGRVTGSEYRQL
                     EGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVIDGGTRFVVGWSLD
                     LAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGILPRLGINHPTGIA
                     GNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRSAFTALQQGKRLN
                     ARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPVKPNGKHYSPAEFRKKRLAEED
                     TEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGRKVLISYDIHDAE
                     RITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAEEKIRDAEDEVVN
                     VLEHKPQEPWLENIYRPVGNTVTVQ"
BASE COUNT          358 a          364 c          420 g          283 t
ORIGIN      
        1 gcctttaaca aggcgcagaa cgccgctgaa cgcctgcttt tactggcacc gggtaagcgc
       61 caggaaataa aagccgaaga tattaactgg ctgcccgaat ttctggcgca gtatcgccag
      121 tcaaacggcc gaccaatgac cgaggcttac gaggattttg tcgctgaatg gcagcaccgg
      181 cacgctgatg agccttatat gctcgatatc atgccgtctt atgacaccat tcgccgcgca
      241 atgaagaaac tgccggaagt ggtgaaacaa aaaggccggg tgaccggcag tgaataccgc
      301 cagcttgagg gattcacgcg ccgcgactgg tcaaaaatgc cggtgaatta tgtctggatt
      361 ggtgacggtc acggcatgaa gctgaaatgc gcacacccgg ttcacggtcg gccatttgca
      421 ccggaagtga cctttgttat cgacggtggc acgcgctttg tggtgggctg gagccttgac
      481 ctggctgaaa atgttttcgc cgtagccggt gccatacagc acggcattcg ccatcacggc
      541 aaaccgtttc tgtattactc ggataacggc tccggggaaa ccgccgacat cctggataag
      601 gaggttgtgg ggatactgcc gcgactgggg attaatcacc cgaccgggat tgccggtaac
      661 ccgcagggac ggggcattat cgaacggctt aaccgcacat taccgatgcg catagcccgt
      721 aaataccgca cctatttcgg gaaaggtgca gatcgcgaga cgttacgcaa aaccaaccgc
      781 gatttacgct cggcatttac cgccctgcaa cagggcaaac ggctgaacgc ccggcaacag
      841 tcagcgatgc gtgatttacc gtcctgggct gaactgattg atgccattcg tgatggtgtt
      901 gagtggtaca acaaccggcc gcacgatgaa ttaccggtga agccgaacgg caagcattac
      961 agcccggcgg agttcagaaa aaaacgcctg gcggaagagg acacggaaat tgaatggctg
     1021 tccgatgttg agctgcggga tatgttccgg ccgatggtgg aacgccctgt aagacgctgt
     1081 gaaatacgct ggctgaataa tatttactac gcgcccgagc tgcgtgacga gcatggccgt
     1141 aaggtgctta tcagctatga cattcatgat gccgaacgaa ttaccgtacg tcgcctggat
     1201 ggcagcgtga tttgcgaggc ggtatgggac ggtaataaac gcgaagcctt cccggttagc
     1261 gcggaatact acaaacagca gcaacgcctt aaaggcatgc gtaaacgcgc agaggaaaaa
     1321 atccgtgatg ccgaggatga ggttgtcaac gtgctggagc acaagccgca ggagccttgg
     1381 ctggaaaaca tataccgccc tgtgggtaat acggtgaccg ttcag
//
LOCUS       DAZUFB010000326         1423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZUFB010000326 DAZUFB010000000
VERSION     DAZUFB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.336"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1395
                     /locus_tag="ACHY3M_005416"
     CDS             <1..1395
                     /locus_tag="ACHY3M_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFS4320088.1"
                     /translation="GDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDFVAAGQAARLMC
                     PHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFWMEGPAAAFQTW
                     EQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSELLEQRAEPFPR
                     RSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRHSLRCSPNGESL
                     PVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAGVTDNAYRFWRR
                     CRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVALWLVQTDAFKDR
                     VNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGANEAFDLLVYAD
                     ALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEKRRRRKKKLRTE
                     RAEDNPWITSGGWL"
BASE COUNT          317 a          347 c          473 g          286 t
ORIGIN      
        1 ggagaccgcc gccggtggta ctggccgtgt ccgcactgcg gggaatattt tcagccggtg
       61 atggataaca tgaccggata ccggaataac ccggattttg tggctgccgg tcaggctgcc
      121 cgtctgatgt gtccgcattg tcgcgggctg attgcccctg agcagaaacg cgaactgaat
      181 aaccagggga tctggcttcg tgaaggtgaa cgggcggcgg cggacggcag tatcaccgga
      241 acgccacgaa actcccggat tgcgtcattt tggatggagg ggccagctgc ggcgtttcag
      301 acctgggaac aactgatttt taaactgctg gcggcagaag aagagtatga gcgaaccggc
      361 agtgaagaga ccctgaaagc ggtggtgaac accgatatcg gacgacccta tctgccccgt
      421 tcagccacgg aacagcgtaa aagtgaactg cttgaacagc gtgccgagcc gtttccccgg
      481 cgatctgtgc cggatggtgt gcgttttatt gaggcaacgg ttgacgtaca gggcggtaaa
      541 aatcgccgtt ttgttgtgca gatcaccgga tacggagagc agggggaacg ctggattgtt
      601 gatcgctaca acatccggca ttcactgcgc tgcagtccca acggtgaaag tctgccggtt
      661 gatccggcgg catatccgga ggactgggat ttgttgctga cggatgtgtt ccataaaaca
      721 tggccgctgg cttctgatcc ggatgtgcgc atgcgtctga tggccatggc ggtggatacg
      781 ggaggggaag ccggggtgac agataacgcc tatcgtttct ggcgtcgttg ccggagtgac
      841 ggactgggca acagggtgtt tctgttcaag ggggatggac ttcgccgtga caggctgatt
      901 aaccgaacct tcccggataa taccggcaga agtgcccgcc gtgccagagc cagtggcgat
      961 gtcgcgctgt ggctggttca gacggatgcg tttaaggatc gtgtaaataa tgccctgtgg
     1021 cgtgacacac cagggccgaa ctatatccac tttcccgact ggctggggcg gtggttttac
     1081 gatgagctga cctatgaaga gcgcggcagt gacggaaaat ggcgaaaacc gggcaggggc
     1141 gctaacgaag cgtttgacct gctggtttat gcggatgcgc ttgccgttct gcatggttac
     1201 gaaaagatcc gctggccctc cgcaccggac tgggcacagc gggaaacgtg gctcgtcttc
     1261 ccgcaggagc gttctggtga aacggtatcc ccggaactga cggccggggc agaaaaacgc
     1321 cgtcgccgga agaaaaaact gcggacggag cgtgcggaag ataatccatg gataacatca
     1381 ggaggctggt tgtgagcaca gaagaagcca gagaaatgat aca
//
LOCUS       DAZUFB010000327         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZUFB010000327 DAZUFB010000000
VERSION     DAZUFB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.337"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..103
                     /locus_tag="ACHY3M_005417"
     CDS             <1..103
                     /locus_tag="ACHY3M_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4320089.1"
                     /translation="DGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            153..>1417
                     /locus_tag="ACHY3M_005418"
     CDS             153..>1417
                     /locus_tag="ACHY3M_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFS4320090.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALT"
BASE COUNT          316 a          395 c          434 g          272 t
ORIGIN      
        1 tgatggcaaa gtgttcctta cgcaggcgca gctggcgatg ctgctggaag gtatcgactg
       61 gcgacagcct aagcggctgc tgacctccct gaccatgctg taaatctctt tatcctggtt
      121 gtcacagaat aagcccggta aaatacgggc ttatgaacga catctcttct gacgacatct
      181 tcctgctgaa acagcgcctg gccgaacagg aagcgctgat ccacgccctg caggaaaagc
      241 tgagcaaccg ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga
      301 tgaacttcgg cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc
      361 tgaaccggct tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac
      421 agcgtccgtt gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg
      481 aaaagcgact gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc
      541 tgggcgagga taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga
      601 cggtacggga aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt
      661 cgcggcccat cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga
      721 agtatgcaga gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg
      781 agctgaggcg ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccgc
      841 tggaagaggc gcttcatggc tatgtcatga ctgacggcaa actccatgcc gatgataccc
      901 cggtccaggt actgctgccg ggtaataaga agacgaagac cgggcggttg tgggcgtatg
      961 ttcgtgatga ccgcaatgca gggtcagcgt tggcacctgc agtgtggttc gcttacagcc
     1021 cggacagaaa aggcatccat ccgcagactc atcttgcctg cttcagcggt gtgctgcaag
     1081 cggatgcgta cgccgggttc aacgagctgt atcgcaatgg tgggataacg gaagctgcct
     1141 gctgggctca tgcccgccga aagatccacg atgtgcacgt ccgcatcccg tcagcactga
     1201 cggaagaagc cctggagcag atcggtcagt tgtacgccat agaggcggat ataaggggaa
     1261 tgccggcaga gcagcggctt gctgaacgtc agcgaaaaac gaaaccgttg ttgaaatccc
     1321 tggaaagctg gttgcgtgaa aagatgaaga ccctgtcgcg acactcagag ttggcgaagg
     1381 cgttcgcgta cgcacttaac cagtggccgg cactgac
//
LOCUS       DAZUFB010000328         1398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZUFB010000328 DAZUFB010000000
VERSION     DAZUFB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.338"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..878)
                     /locus_tag="ACHY3M_005419"
     CDS             complement(18..878)
                     /locus_tag="ACHY3M_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFS4320091.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1061..>1398)
                     /locus_tag="ACHY3M_005420"
     CDS             complement(1061..>1398)
                     /locus_tag="ACHY3M_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4320092.1"
                     /translation="DMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAE
                     RRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVY
                     KILEDERAS"
BASE COUNT          350 a          362 c          312 g          374 t
ORIGIN      
        1 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
       61 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      241 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      301 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
      361 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      421 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      481 gcagcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      541 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      601 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc accacatagc
      661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      841 aagggaataa gggcgacacg aaaatgttga atactcatac tcttcctttt tcaatattat
      901 tgaagcattt accagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1021 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     1081 caagaatttt ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac
     1141 cagtgccctt ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa
     1201 atttgattcc tttcagcttt gcttcctgtc ggccctcatt cgtgcgttct aggatcctcc
     1261 ggcgttcagc ctgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt
     1321 cggtactgat cccgtcatca atgaaccgga ctgccacgcc ctgagcgtca aattccttta
     1381 tcagttggat catatcgg
//
LOCUS       DAZUFB010000329         1395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZUFB010000329 DAZUFB010000000
VERSION     DAZUFB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.339"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..370)
                     /locus_tag="ACHY3M_005421"
     CDS             complement(<1..370)
                     /locus_tag="ACHY3M_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320093.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLL"
     gene            complement(409..>1395)
                     /locus_tag="ACHY3M_005422"
     CDS             complement(409..>1395)
                     /locus_tag="ACHY3M_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4320094.1"
                     /translation="TRGGSYGVDPQIHNNDKAKALKSATEHTLQAVARKWLDEKVKTS
                     GISQDHAADIWRSLERNVFPGLGNVPINEIRPKLLKQHLDPIEQRGVLETLRRIISRL
                     NEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPALPPSELPRFMESLTNASIRLETR
                     MLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPADFMKMKKLHKVPLSKEALRILEL
                     MKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMGFGGELVAHGMRSIARTAAEESGK
                     FRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLMQWWSDYVQAQRSNALVA"
BASE COUNT          385 a          296 c          285 g          429 t
ORIGIN      
        1 ataatagagt tgtgctgtat ataaagccag agttagttaa taactttaaa tttaatgctt
       61 caggattttc actgagaaag tgaaaggcat aaactaagta tacagctaaa gtagccgttc
      121 caattgttgg tccaatatcg ccttgatcat gaggttcaag gtttacattg aacttgaaga
      181 ttagccattc aaatccccaa gtaaaaaaaa caactaatat catggctgcg aataatttcg
      241 cgatgttatc tgctgatgga ttcatcagaa cagattgttt ctgaaaaaat tcacaaagtg
      301 agagtccgaa aaaaataaaa aaaagtggtc tggaactaaa tttttcaaaa actttgaata
      361 gggtttccat tttctttacc ttcaggatta tattagctat tctgatactt aggctaccag
      421 agcatttgat ctttgagctt gaacgtaatc actccaccat tgcatcaaac tctgtcgctc
      481 tatcagatat tctgcacgat tgtatgctgc gataatttca tcttttttcg agtgggcaag
      541 cgctgcctca agaacttcag ctctgaattt accagactcc tctgccgctg ttcgtgcaat
      601 agaacgcata ccgtgagcta caagctcgcc tccgaacccc attcggatga tagctgcgtt
      661 ggctgtttgt tcatgcatat gattaagagg cgcttttatg ctggggaaaa cccattctct
      721 atgcccactt attgatttca ttaattcaag gatgcgcaaa gcttctttac tcaaaggaac
      781 tttgtgaagc tttttcattt tcatgaaatc agcaggaatg ttccaaatgc tgttggttgt
      841 atcaatatca gaccaccttg cgcgaacggc ttcacccgga cgaacccatg tcaacaattg
      901 ccattcaatt agcatacgtg tttccaaccg gattgacgca ttcgtcaaag attccataaa
      961 ccttggcaat tcgcttgggg gaagggcagg catattttgc ttttttggtt tactgaatct
     1021 ttgaccaagg ttgtcagccg ggttgaactc aataagttct tcagtagctg cccaccggaa
     1081 gatttcattc agacgtgaaa tgatacggcg tagagtttcc aatacgcctc gttgctcaat
     1141 aggatcaagg tgttgtttta agagcttagg tcggatctca ttgataggga cattacccag
     1201 accgggaaag acatttctct ctaagctgcg ccagatgtct gctgcatggt cttgtgagat
     1261 acctgatgtc tttaccttct catctaacca tttccgcgct acggcttgga gagtgtgctc
     1321 agtagcactc tttaatgcct tcgccttatc gttgttatgg atttggggat caacaccata
     1381 tgaaccgccc cgggt
//
LOCUS       DAZUFB010000330         1384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZUFB010000330 DAZUFB010000000
VERSION     DAZUFB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.340"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1384
                     /locus_tag="ACHY3M_005423"
     rRNA            <1..>1384
                     /locus_tag="ACHY3M_005423"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          347 a          313 c          428 g          296 t
ORIGIN      
        1 atgttggccg ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc
       61 cggaaaacta aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc
      121 tgcttccagg aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca
      181 ggtggtcagg tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa
      241 atggtgccgt aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg
      301 agctgaaatc agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt
      361 gcaaacacga aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga
      421 tggggtcagc gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata
      481 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa
      541 tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag
      601 tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat
      661 tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga
      721 gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgttgaccc gtaatccggg
      781 ttgcggacag tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga
      841 ggagcacgaa ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc
      901 cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt
      961 ggttctgaat ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg
     1021 ataccgccca agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca
     1081 tcctggggct gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag
     1141 ctgggtttag aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg
     1201 aggggggctg ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt
     1261 catgccaatg gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa
     1321 gcacgaaact tgccccgaga tgagttctcc ctgactcctt gagagtcctg aaggaacgtt
     1381 gaag
//
LOCUS       DAZUFB010000331         1383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZUFB010000331 DAZUFB010000000
VERSION     DAZUFB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.341"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            392..922
                     /locus_tag="ACHY3M_005424"
     CDS             392..922
                     /locus_tag="ACHY3M_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4320095.1"
                     /translation="MKKILLVAGAALALAGCGEKGDFEKAINTKIGQDKYCFSLDNNN
                     TSFPIRLAKPLLDSTGTGTNSVILDGFIEQGLMVFEQGYDSNVLGITEEGKKAKVWST
                     TDGACVGRRAVDEIKEWTEPGNGNQKVVRVTYTWKLVDVPGWIDKKAFASVKGMNEPA
                     DGAINLVKTSNGWRAN"
     gene            933..1208
                     /locus_tag="ACHY3M_005425"
     CDS             933..1208
                     /locus_tag="ACHY3M_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320096.1"
                     /translation="MENNIIKCPFCFKESQRGVHVCTGCLATVLYGAYPKWYAAVVLL
                     LAFGLSIIIGMSTGMAGATISLPIIAVVGFIAGKAIFSDNVVFRRRM"
BASE COUNT          405 a          249 c          329 g          400 t
ORIGIN      
        1 gtttccttgt tgatgacttc catatccacc tcaccagatc ctactgaacg acctacgcgt
       61 agcatatcat tttgtgaaca gatctcaacg aaatgatcga tataaaagat cgataatgag
      121 ttattgatag attgcagcga ttaaatatgc gatatcgatc ggtaattaat tgctataatc
      181 aatcgaatat ctatttatga tagataaaag ctatcataag gttgtttttg atcgttttaa
      241 acgatatgac gagacgctaa aagaattgaa tcctatcaat tggttaagcg gtggttttgc
      301 catgtgatat cagccggatc ccgcaacaac tctagccctg accggattag gtcaaaattt
      361 aggcactttg attatcagga tggataagca gatgaagaag attttactcg ttgctggcgc
      421 agctctggca ctggcgggat gtggtgagaa aggtgatttt gagaaagcga ttaataccaa
      481 gattgggcag gacaaatact gcttctcgct ggataacaat aacaccagct tccccatccg
      541 tcttgccaag ccactacttg attcgactgg cacagggacg aattcagtga tcctcgacgg
      601 ttttattgag cagggcctga tggtttttga acagggctat gactcgaatg tattagggat
      661 aacagaggaa ggcaaaaaag ccaaagtctg gagcacaaca gacggtgcct gtgttggtcg
      721 tcgtgctgtt gacgagatta aggaatggac tgagccgggc aacggtaatc agaaagtagt
      781 tcgcgtgact tatacatgga agctggttga cgttccgggc tggattgaca aaaaagcctt
      841 cgccagcgtt aaaggaatga atgaacctgc tgatggtgca atcaatctcg ttaaaaccag
      901 taatggctgg agagccaatt aaggaaacca atatggaaaa caatatcatc aaatgcccgt
      961 tctgtttcaa agagagccag cgtggggttc acgtctgtac aggctgttta gcgacggtgt
     1021 tatatggtgc ctacccgaag tggtatgctg ctgttgtttt gttattggct tttggtctat
     1081 ctattattat tggtatgtca acaggcatgg cgggggctac tatcagccta ccaataattg
     1141 cggttgttgg gtttatagct ggtaaggcga tattctctga taatgttgtg tttaggcgaa
     1201 gaatgtagct agcagctata aataataaac cactaccaac ccgcattgtg gtggtggttt
     1261 attatcttat tccctgaaat aaaactcaca tccttattga atgttgttga aatattctga
     1321 aattaagtcg gagtttttaa aatatgagtc tagaacctga aataataaaa actcgttatt
     1381 gtt
//
LOCUS       DAZUFB010000332         1357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZUFB010000332 DAZUFB010000000
VERSION     DAZUFB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.342"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            188..1333
                     /gene="espG"
                     /locus_tag="ACHY3M_005426"
     CDS             188..1333
                     /gene="espG"
                     /locus_tag="ACHY3M_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFS4320097.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSLG
                     LWNPTYSRSERQSFQELLTVLEPVSPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKIFVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEIDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT"
BASE COUNT          434 a          286 c          250 g          387 t
ORIGIN      
        1 acgcatcact tgcaatcaat tatccgtcat tagattaata ttttcactta tttttcagaa
       61 ggtaaaaata actttctatg cgtttaaaat acccgctata aatatcttgt atattattga
      121 cactatatga tgctatatga tacttgttgc caaattgttc attacaaacc agataggaga
      181 atctctcatg ataaatggac ttaataatga ctccgcatct ttagttttag atgctgcaat
      241 gaaagttaat tctgggttta aaaaaagctg ggatgagatg tcatgcgctg aaaagttact
      301 taaagtactt agtcttggtt tatggaatcc aacgtacagt cgtagtgaaa gacaatcatt
      361 tcaagagttg ttaaccgttt tagagcctgt atctccactt cccaatgaat taggcagagt
      421 atctgctcgt ttttcagatg gctcatcctt aagaatttcc gtcactaaca gcgaaagtat
      481 tgaagccgag attcgcacac cagataatga aaagattttt gtgctcctag agtcaaacga
      541 acaaaatagg ttattacaat ctttacccat cgatcgccac atgccataca ttcaggttca
      601 tcgtgcctta tctgagattg acctgactga tactacctca atgcgcaatc tacttggttt
      661 tacgtcaaaa ctatcaacaa ccttgattcc gcataatgct caaacagatc cgctttccgg
      721 gcctacacca ttcagctcta tctttatgga tacatgtcga ggactcggca atgcaaagct
      781 ttcactcaat ggtgttgata tacctgcaaa tgcacaaatg ttgcttcgcg atgcactagg
      841 acttaaagac acacactcat caccttcccg gaatgttata gatcatggta tttctcgcca
      901 tgatgcagag caaatagcaa gagaaagcag cggcagcgat aatcagaaag ctgaagttgt
      961 ggaattttta tgccatccag aagcagcaac ggccatatgc tcggctttct atcaatcttt
     1021 caatgtgcca gccttaacgt tgacacatga aaggatctct aaagccagtg aatacaatgc
     1081 ggaaagatcg ttagatacac ctaacgcttg cattaacata agtatctctc aatcatcaga
     1141 tggaaacatt tatgttacca gccataccgg ggttctgata atggcgccag aagaccgccc
     1201 caacgagatg ggcatgttga cgaacaggac ttcttatgaa gtgccgcaag gtgtgaaatg
     1261 tacaatcgat gaaatggtaa gagcgctaca accaaggtat gctgcatctg aaacgtactt
     1321 acaaaacact taaaataaaa ctttattaat caaaacc
//
LOCUS       DAZUFB010000333         1356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZUFB010000333 DAZUFB010000000
VERSION     DAZUFB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.343"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..173
                     /locus_tag="ACHY3M_005427"
     CDS             <1..173
                     /locus_tag="ACHY3M_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFS4320098.1"
                     /translation="MKSKVDAHPYYDGLGKGIKRHFNYSSLHNYNAFCDFIEFKHENI
                     IPNTSMYTSSSW"
     gene            222..896
                     /gene="nleE"
                     /locus_tag="ACHY3M_005428"
     CDS             222..896
                     /gene="nleE"
                     /locus_tag="ACHY3M_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFS4320099.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKHINN
                     KLSKIE"
     gene            1271..>1356
                     /locus_tag="ACHY3M_005429"
     CDS             1271..>1356
                     /locus_tag="ACHY3M_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320100.1"
                     /translation="MMQKAIYKTHDKNYARRLTAMLMLHRSAR"
BASE COUNT          474 a          236 c          265 g          381 t
ORIGIN      
        1 ttatgaagag taaagttgac gctcatccat attatgatgg tctaggaaag ggtatcaagc
       61 ggcattttaa ctattcatcg ttacacaatt ataatgcttt ttgtgatttt attgaattta
      121 agcatgaaaa tattataccg aataccagta tgtataccag cagttcatgg taagtcttcc
      181 ggttgtggtt gaaaaatatc gttctttagg tatagattca gatgattaat cctgttacta
      241 atactcaggg cgtgtcacct ataaatacta aatatgctga acatgtggtg aaaaatattt
      301 acccggaaat taaacatgat tactttaatg aatcacccaa tatatatgat aagaagtata
      361 tatccggtat aaccagagga gtagctgaac taaaacagga agaatttgtt aacgagaaag
      421 ccagacggtt ttcttatatg aagactatgt attctgtatg tccagaagcg tttgaaccta
      481 tttccagaaa tgaagccagt acaccggaag gaagctggct aacagttata tccggaaaac
      541 gcccaatggg gcagttttct gtagatagtt tatacaatcc tgatttacat gcattatgtg
      601 agcttccgga catttgttgt aagatcttcc ctaaagaaaa taatgatttt ttatacatag
      661 ttgttgtgta cagaaatgac agccctctag gagaacaacg ggcaaataga tttatagaat
      721 tatataatat aaaaagagat atcatgcagg aattaaatta tgagttacca gagttaaagg
      781 cagtaaaatc tgaaatgatt atcgcacgtg aaatgggaga aatctttagc tacatgcctg
      841 gggaaataga cagttatatg aaacacataa ataataaact ttctaaaatt gagtagagaa
      901 gctcagccac ttgtgtaggg gggctaaaat taattctatt tcattatatg agaacaccaa
      961 taagaataat aatatttgat cacaactgaa gatgcttttt acaatgaaga attattgtta
     1021 aaaaacgctt tgttgaatca accgaactgt tgcttagttg caggatcaca tcacgtattt
     1081 tcctgaaaac gcaggctgct tagtagcaaa gcagaagttc aaaatcacca actaaatagc
     1141 tgcgccgaat agtagatcaa tgaaagggaa ctcagcccgg attgtgcgat ctgatcaatc
     1201 gccaaaaatc cccccctaac cggactgagc gatgccgatc atagcaccaa ttccccgcga
     1261 cgaatgacgc ctgatgcaaa aagcaatcta taaaactcac gataaaaatt atgcccgcag
     1321 gctgaccgcc atgctgatgc tgcaccggag cgctcg
//
LOCUS       DAZUFB010000334         1347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZUFB010000334 DAZUFB010000000
VERSION     DAZUFB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.344"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1127
                     /locus_tag="ACHY3M_005430"
     CDS             3..1127
                     /locus_tag="ACHY3M_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFS4320101.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            1127..>1347
                     /locus_tag="ACHY3M_005431"
     CDS             1127..>1347
                     /locus_tag="ACHY3M_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFS4320102.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYG"
BASE COUNT          309 a          392 c          389 g          257 t
ORIGIN      
        1 gaatgaagac gaaaaacacg cccaaacttg cttatgccat tctgaatgcc atcagcctgt
       61 ccgcagacgg ggacggtgac tggtgtcaga tcatgccagc cggtcgggta aaggcccgtg
      121 acggtcgccc ggaaaaaccg gccgagggct ggctgatcaa ccatgcagcc gttgagcgca
      181 tggtctcccg tgttgtggcg ctcaatcagc cggtgaaaat tgactacaac caccagaccc
      241 tgattgaggg acatccggca ccggctgccg gttttgtgat ggcctcaccg gagaattttc
      301 gcttcagtga agagcgaggt ttcgaggtgc gcccgaagtg gaaccctccg gcccttgaac
      361 atctgcgtaa taacgaattc ccctggtttt caccggtgat tggctatgac gagagcaccg
      421 gcgaacctgt cgaacttcgg atgctggcta ttaccggtga ccccggtctg accggcatga
      481 atcctgtcgc cgcactgtcg gcggatgacc tttataacgc cttaaaccct cctttaaagg
      541 atacctccat gaatgagcaa ttacgccagt tgctgacggc gctcggtctg accgtggctg
      601 acggtgacga atttacgccg gagctgggca cggcggcgct gtctgccctc accgggatca
      661 aaacccgtgc ggatgcacac gacaacctga aaacacaggt cgccagtctg tcggcagagc
      721 tggaaaccgc aaaaggcaca ccggctggcg gcagtattga tctgacgaaa tacgtgcccg
      781 ttgaaacgta taacgccctg cgtaccgaat acgttgcgct gtcggcgcag cacggcagca
      841 ccacgctgga gcaggtgctg gacaaggctg aatccgaagg gcgcatcttc aaaagcgaac
      901 gcggctatct ggaacaactg ggcgggcaga ttggcgttgc ggcactttct gcacagcttg
      961 acgcccgcca gccggttgcg gccctgaccg gccttcagac cgacaccgtg accgtgccgg
     1021 ataaaaagac tgccaccgct gtgctgtcgg ctgaagatat cgcggccgcc aaactgctgg
     1081 gtaaaaccga agccgagttc ctgaaaatga aagaggaaat gcaataatgc ctaccccgat
     1141 tacaccggcg atgatcaccg ccctgatgac gggctaccgt tctgattttc aggccgggat
     1201 gtccatggcc ccgtcgcagt acaaaaaaat tgcgatgacc gtaccgtcca cgtcgaaatc
     1261 caacacctac ggctggctgg ggcagttccc gcagttccgt gagtggatcg gctcccgcgt
     1321 catcgagaag atgaaggcct atggcta
//
LOCUS       DAZUFB010000335         1344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZUFB010000335 DAZUFB010000000
VERSION     DAZUFB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.345"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..340
                     /locus_tag="ACHY3M_005432"
     CDS             <1..340
                     /locus_tag="ACHY3M_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320103.1"
                     /translation="CNRPSFVVSGDDGKITISENGKVTLPSHQHSETLIEFAIDYLKN
                     NKKQGLMQRIGRCMGYLQVAAEIEALASGADKDAIVREALLRNFNTPPFKKVPAYWLH
                     PGLTYLKVRI"
     gene            377..910
                     /locus_tag="ACHY3M_005433"
     CDS             377..910
                     /locus_tag="ACHY3M_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4320104.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1070..1207
                     /locus_tag="ACHY3M_005434"
     CDS             1070..1207
                     /locus_tag="ACHY3M_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320105.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            1209..>1344
                     /locus_tag="ACHY3M_005435"
     CDS             1209..>1344
                     /locus_tag="ACHY3M_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4320106.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKVSEL
                     E"
BASE COUNT          344 a          288 c          387 g          325 t
ORIGIN      
        1 gtgtaaccgg ccatcctttg ttgtatccgg tgatgacggg aaaatcacca tatcagaaaa
       61 tggaaaagta acactgccat cgcaccagca cagtgagacg ctcattgaat ttgccattga
      121 ttacctgaag aacaataaaa aacaggggct gatgcagcgc attggtcgtt gcatggggta
      181 tctgcaggta gccgctgaga ttgaagcgct ggccagtggt gctgataagg atgcaattgt
      241 gcgggaggct cttcttcgta attttaatac tccacccttt aaaaaagtgc cggcttactg
      301 gcttcatccc ggactgactt atctgaaagt gcgtatttag tgggcctggg acagcggctg
      361 aatatttaat atatccatga acgcaaaaat cagatacggc ctgtcggctg ccgttctggc
      421 gctgattggt gcaggggcgt ctgcgcctga aatcctcgac cagtttctgg atgaaaagga
      481 aggtaaccac accacggcat accgtgatgg tgcgggtatc tggaccatct gccgtggagc
      541 cacccgggtg gatggtaagc ctgttattcc tggcatgaag ctgtcgaagg ggaaatgcga
      601 ccgggttaac gccattgagc gtgataaggc gctggcatgg gtggagaaaa acatcaaagt
      661 gccactgact gaaccccaga aagcggggat cgcgtcattc tgcccgtata acattggccc
      721 cggtaagtgt ttcccgtcga cgttttataa acgaattaat gcaggcgatc gcaggggagc
      781 gtgtgaagcg attcgctggt ggattaagga cggaggcaga gactgccgta tccgctcaaa
      841 taattgctat ggtcaggtat cccgtcgtga ccaggagagc gcgctggcgt gctggggtat
      901 cgacagataa gcagaatatt ttgctgaaaa atgacgttgg ccaacgcgga cggataacac
      961 gaaatcctgc gaactggcaa aacctgagtg aataaaagta aaaaccccgt ttgttagcag
     1021 caggtggggt tttgtgtttc ctgactccgg aaaagtcaaa ggagaaagtg tgtttgattt
     1081 tagcaaactg attcgggaga ttcgagtgat ggctgaaaaa ttatccacct ggaagttcat
     1141 tcttatctgg ctggtgtttg tgattatggc ctccggttat ttcatcggtc agatacgctg
     1201 gtggtgaaat gaaccgcgta ctgtgcgtgg tcatcattgc cctgctggtg gcctgtggtg
     1261 cgcttagtct ggggctgaat cattaccgtg ataacgccat aacctacaaa gcgcagcgcg
     1321 ataaaaaagt cagtgagctg gagc
//
LOCUS       DAZUFB010000336         1342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZUFB010000336 DAZUFB010000000
VERSION     DAZUFB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.346"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..479)
                     /locus_tag="ACHY3M_005436"
     CDS             complement(<1..479)
                     /locus_tag="ACHY3M_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFS4320107.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKNIKLRVNSTIMA"
     gene            complement(1114..>1342)
                     /locus_tag="ACHY3M_005437"
     CDS             complement(1114..>1342)
                     /locus_tag="ACHY3M_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFS4320108.1"
                     /translation="RFLERSASQGNVTAQFNYGVFLSDTNPTLSEYYNLNRAIYWMEF
                     AVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          396 a          272 c          215 g          459 t
ORIGIN      
        1 atggccataa tagtactatt aactctcaat tttatatttt tatcatcact acaaatatta
       61 actgaattta tatcaatgat ttttttattc ttatctttta ttttatttac tgtttcaatg
      121 caatcagcgg caagttggag tggatttaga tttttcagat atggtcttat gatctttccc
      181 ttacttaata aatagcatgt ataggattcg gaaagtccag catctatttt gtacctcatt
      241 atattagata aggatgtgag aaattccttg ttatcattcg cagccatata tgccagtccg
      301 agttcagcct ctgcggcctt acaggcaaaa tagcttagtg aacacgcttt tcgtccctct
      361 ttagtactgg ctgatattag tgcccataat attgctttgt ctgccaaatc taactctctc
      421 tgaaattgtc ttgcagtaac tgcactcgca gaaatagaga aaaaaataca attgttaata
      481 accttgccat attttttagt ccgtaactgt taatgtgcta tctggctctg aaaatattaa
      541 ctcatttaat ttttgcatgt cattagataa accagttata caaccctcgg aacagaatcg
      601 aggtcctaaa gacggaccaa aagcgtttaa acaactatgt attgaaaatc cattgcgatt
      661 ataagttcct gtattaactg acggtactaa tcgtatgccg tcaggttttc tattgcttgc
      721 tcccgggtca ttaacattct aactccatag gccttgaggt attggccatc tatttataaa
      781 tgtgtacaac ggggattatt tttacattgc atattattac cagaagcgac tggaatacta
      841 agcacatcat aaaaattatc atcacatggt atatctgaaa cacacgaaag catacctaaa
      901 gttatactgt ataaacaggt ggctaatccc aaggggatcg attccagaaa tcgggttcaa
      961 cggatactga taaaaattcc acctaccttc cagcccaatc ggagcctgag tgttataccg
     1021 cccctgctgc ggttaatgtt accgataata gttatagtac agccctgatt catcatcata
     1081 ctactgctgc ggcagtctga tgaactattg cttttacgga ttctccttat tcttccttct
     1141 atccattcgc tttagctttt tgagctcttg aagtttgctc tttgcatcaa tattaccgtt
     1201 atttacagcg aattccatcc aataaatagc tctattcaaa ttataatatt ctgataatgt
     1261 tggatttgta tctgataaaa agaccccata attaaactgc gctgtaacat tcccctgaga
     1321 tgctgatctt tccaagaatc gc
//
LOCUS       DAZUFB010000337         1304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZUFB010000337 DAZUFB010000000
VERSION     DAZUFB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.347"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..712
                     /locus_tag="ACHY3M_005438"
     CDS             <1..712
                     /locus_tag="ACHY3M_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4320109.1"
                     /translation="KFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAV
                     VARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQPTGA
                     VWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDIY
                     LADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRR
                     TAAVAVITGVNYGSASGG"
     gene            757..978
                     /locus_tag="ACHY3M_005439"
     CDS             757..978
                     /locus_tag="ACHY3M_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320110.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            924..>1304
                     /locus_tag="ACHY3M_005440"
     CDS             924..>1304
                     /locus_tag="ACHY3M_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320111.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRN"
BASE COUNT          268 a          338 c          425 g          273 t
ORIGIN      
        1 gaagtttgat tttgaatcca tcaccttcag tcatgcgaaa gtgtcggcca ttgcggtact
       61 gacggaagaa ttgatccgtt tttccagtcc ggctgctgat gcactggtcc gtaatgcgct
      121 ggcggaagcg gtagtggcgc gtctggatac agactttgtg gacccgaaaa aagccgcggt
      181 ggcggatgtc tccccggcgt ccatcaccca tgatgtgaag ggcacggcat caaccggtaa
      241 cccggatgcg gatgccgagg ccgcgtttgg ccagtttgtg acggcaaatc tgcagcccac
      301 tggtgctgtc tggctgatgt ccagcaccaa tgccctggcg ctgtccatgc gtaaaaatgc
      361 gctgggtcag aaggaatacc cggacatgac cctgcttggc ggcaccttcc agggcctgcc
      421 ggtgattgtc tcccagtacg tgggtgacca gctggtgctg gtgaatgcgc cggatattta
      481 tctggctgat gacggcggtg tggcggtgga tatgtcccgt gaagcgtcac tggagatgca
      541 gtctgaaccg accggcgaca gtaccacgcc gtccccggtg gagctggttt ccatgttcca
      601 gacaggcagc gtggccatcc gtgcggagcg ctggatcaac tggcgtcgtc gccgtactgc
      661 ggcggtggcg gtgatcaccg gagtgaacta cggcagtgcg tccggcggct gagtctgatg
      721 aggaggacgg gaggcgtgcg cctcccgtaa caggttatgg caaagatccg atatctgcag
      781 ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg cacagccgtg tgcggaggtg
      841 ctggttcgcc tggggaaggc ggagtacatc acagtgcgac gtccggcagg tcagaaaaag
      901 aaacgtgatg cggagcatgg cgaatgtgga accttttgcg gcgaacccga aaaaaccaga
      961 aatcaggacg tgatgtaaaa gaggtgggct ggaccagcct gtttcaggcg gtggctgagc
     1021 cttttgccgg cgcctggcag cagggcgtga aagccgatcc ggaaagtgtc ctctcctttc
     1081 atgcggtgtt ttcttgcatt tcgctgatat cccaggatat cgccaaaatg cgactgcgcc
     1141 tgatgcagac cgatacacag gggatccgcc gtgaaaaacg gcagggggat attgcccgtc
     1201 tctgtcgtcg tcccaatgca cagcagaatc gtatccagtt ttttgaactg tggctgaacg
     1261 ccaaactgcg tcacggcaat acggtggtgc tgaaaatccg taac
//
LOCUS       DAZUFB010000338         1303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZUFB010000338 DAZUFB010000000
VERSION     DAZUFB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.348"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..282)
                     /locus_tag="ACHY3M_005441"
     CDS             complement(<1..282)
                     /locus_tag="ACHY3M_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFS4320112.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVN"
     gene            complement(355..930)
                     /locus_tag="ACHY3M_005442"
     CDS             complement(355..930)
                     /locus_tag="ACHY3M_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFS4320113.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(1043..>1303)
                     /locus_tag="ACHY3M_005443"
     CDS             complement(1043..>1303)
                     /locus_tag="ACHY3M_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4320114.1"
                     /translation="LRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENGIIP
                     EQDRVITQVVILDADKKQIQCMVRPLQILRADGRWENIGGMK"
BASE COUNT          379 a          344 c          224 g          356 t
ORIGIN      
        1 attaacacta cttaaaaaat cgacacctcc atttaattgc ctttccagag ccacagccct
       61 tctctccatt cgatgctcac gccccaatag cctatccagt aagcctcctc tcaccggttc
      121 aactgaaaat ccatccagta gctggacata gtgaacagaa tattggccac taccaatgga
      181 tattcttatt ccactttcaa gtctggcagc atcctgcaaa gactgtactc ctgcaggaga
      241 taattgagat atacttggga tacttaaggt ggtaacaggc ataaaaacct cattcagtaa
      301 ttcatatcag tttgcaggat gtttcattaa ataaaataaa gggtaatgtt catttcaatt
      361 accctttata acgaagtttc ctctggtatc gtcataaatg cattcttcat gttttacaat
      421 tattgatgcc gttattggtt cccgggtcag tgggtggggt aagccttcac caaccaaacg
      481 agaaaatgca gcggcatcaa ataaagtaca tacatctgaa ccatctgaat tcttcacaaa
      541 aatacctttt tcaggctgct ccagtgtaat tggacattga atagcctcaa gcgggcactg
      601 aagtctctct ggacaaacag aaaacttaca ttgagatatt ttgttctgaa tactctcttg
      661 cgattgtgct tcacctgagt ccgaaagcat acttagcatt ctctcaccaa gggctcgagg
      721 accactatta aatcccaaaa gcaataactc agaaagcaat ccgccattac cgccactgga
      781 tgaaaagcgc ccattagtag aatcatacac aatactgaca gtttcaccac cgactgtaat
      841 atctccgcgc ccattggcta caattcgggc acgaacaacc tccaccccaa gattaaatga
      901 atgtgatcta atatctgagg ttaatggcat aacaaagctc cctattttaa tttgaactcc
      961 agacttaaat agctgtaaca aacatccccc atacgtgaca acaaaaacca gagcaggact
     1021 ccggtttttg tgaacccgtc ggctatttca tcccgccaat attttcccac ctcccgtcag
     1081 cacgtaggat ttgcagcggt cttaccatgc actgtatctg ctttttatcc gcatccagta
     1141 tcaccacctg tgtgattacc ctgtcctgct ccggaataat gccattctca tctgactcca
     1201 ggatgtctgc cggccccagt cgcagttgta ctgtaagcga ctgcccgtgt tcacagtcat
     1261 catgctttcc gcaaccacac agacgctcca taagctttct caa
//
LOCUS       DAZUFB010000339         1268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZUFB010000339 DAZUFB010000000
VERSION     DAZUFB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.349"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..1247
                     /locus_tag="ACHY3M_005444"
     CDS             join(34..325,325..1247)
                     /locus_tag="ACHY3M_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFS4320115.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    315..431
                     /locus_tag="ACHY3M_005444"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          315 a          300 c          380 g          273 t
ORIGIN      
        1 gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatactcg tttttccccc
       61 gaagtccgtc agcgggcgat tcgtatggtt ctggaaagtc aggatgaata tgactcacag
      121 tgggcggcaa tttgttccat tgccccaaag attggctgta cgccggagac tctgcgtgtc
      181 tgggttcgcc agcatgagcg ggataccggg ggcggtgatg gtgggctcac cagcgctgaa
      241 cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc
      301 cttcgccagg cttccgctta ttttgcgaag gcggagttcg accgcctctg gaaaaaatga
      361 tgccactgct ggataagctg cgtgagcagt acggggtcgg accggtatgc agcgaactgc
      421 atattgcccc gtcaacgtat taccattgtc agcaacagcg acatcatccg gataaacgca
      481 gtgcccgtgc gcagcacgac gactggctga agagagagat acagcgcgta tacgatgaaa
      541 atcatcaggt gtacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg
      601 tggccagatg tacagtggca cgtctcatgg cggttatggg acttgccggt gttctccggg
      661 gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc
      721 gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc tgattttact tacgtcagca
      781 catggcaggg cttcgtctat gtggcgttta tcattgatgt gtttgccgga tacatcgtgg
      841 ggtggcgggt ctcatcgtct atggaaacga cattcgtgct ggatgcgctg gagcaggcgt
      901 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      961 cactggccta tacggagcga ctaaaagaag ccggattact ggcatcaaca gggagtacag
     1021 gcgactcgta tgacaacgcg atggctgaga gcatcaatgg tctttacaaa gcggaggtaa
     1081 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccacacta acgtgggtgg
     1141 actggtataa caatcgacga ttgctgggaa ggctgggcca tactcctccg gcagaagcag
     1201 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
     1261 acactctc
//
LOCUS       DAZUFB010000340         1264 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZUFB010000340 DAZUFB010000000
VERSION     DAZUFB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.350"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..454)
                     /locus_tag="ACHY3M_005445"
     CDS             complement(95..454)
                     /locus_tag="ACHY3M_005445"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4320116.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1140..>1264
                     /locus_tag="ACHY3M_005446"
     CDS             1140..>1264
                     /locus_tag="ACHY3M_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS4320117.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIP"
BASE COUNT          371 a          253 c          249 g          391 t
ORIGIN      
        1 agatactgac atttttctga ggataagagg tgaatacctc ttatccttaa gtaaatatta
       61 accaggtaac tgtgaataat ttagatgttg ggcgttatga aagtaataaa actccccata
      121 taatgatagc aactaaaatc gtaatcaaca ctgcggctga acccatatct tttgctctgc
      181 ctgataattc gtgatgttct gaaccaattc tgtcaacaac tgcttcaatt gcactgttaa
      241 ttatttcaac aataattacc atcattgccg aaccaatgag taatactcga gtaacagagt
      301 caacatccag ccaaaaggat attatcagtg ctgacagaac cagaatgcac tcctgacgaa
      361 aggctgcttc atagcgccat gccgcacata agcctttcca tgaataaccc gctgctttaa
      421 taattctggt aacaccagtg gtcgtattag ccataaatac ctcttattta attatatgtc
      481 tgaatatgca ttataaaata tcagttaaca ttaacaggat tttgtaataa gccacattta
      541 ttctttcggg aattaaaaaa cataacgaaa ttttgcctca ttctgcattt cattgaaata
      601 gtatgtttct catcgaaagc aagtttgtca atataaattt gtaacagaaa tgactttccg
      661 gggataaaca aaactgttca tggcaatggc tttctctgtc gccaacatgg atacattgtt
      721 ccggataaag cactgatgtc attgactgac gcagactatc aggccatctg atgctttctt
      781 ttccttcacc ggtattgatg atatgcctta tgaaggggga cttattcctg taacccaatc
      841 acaggcactg tgattactgg tttatgattg atgctgattt gagcagatat gcacagcagt
      901 tgtggattca ggggatattt tctgccagat attcatacca gattgatgac ctctttgctc
      961 agatgctctt cgttctgagc cagccaggat tttgtggcca catacattgt tcgccgatgg
     1021 ggaatcgaga caggcgattg gctgtgatgc gattttgccg ctaactgagg atgagtccgc
     1081 ccctcccggg gaattcctgt aagttaagcc gtacctgttg attagcacta aactgggtaa
     1141 tgaatttatc tgccagtatg aatgatgccg gggacggttt tgaactcctg atgtcgggta
     1201 atatgcagga ttttaatatt tgttgtcgtc gggataatca ttatcctgaa cataaactga
     1261 tacc
//
LOCUS       DAZUFB010000341         1263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZUFB010000341 DAZUFB010000000
VERSION     DAZUFB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.351"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1263
                     /locus_tag="ACHY3M_005447"
                     /pseudo
     CDS             1..1263
                     /locus_tag="ACHY3M_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          290 a          307 c          413 g          253 t
ORIGIN      
        1 ggcctacaag attggcgttg gccacccgcc ttccagtgac aacgtggagg cgctggagca
       61 gcagtattat tcccagtgcc tgcagacgct gattgagtcc attgaactgt tactggatga
      121 ggcgctggaa acgggggaaa acgagagtac ggagtttgac gtcaccacgc tgctgagaat
      181 ggacagcgaa cggcgcatga aaacgctggg tgagtcggtg aaaaatacgc ttctcacgcc
      241 caatgaggcc cgtaaacgtg agaacctgcc gccccttgcc ggcggtgatg cactgtatct
      301 tcagcagcag aactacagtc tggaggcgct gtcccgtcgt gatgcccgtg aagatccgtt
      361 tgcgtcgtcc gggaaaagag tttcagccca actgcctgac ggcgcatctg acggtaataa
      421 ggcaatcagt gaaacagagc atggtgcggt gaaagcgatg ttgaggggga tactgcgaaa
      481 atgacggaac gggaactgtc cattattcgt gcactgggag aagaattcgc cacggtgctg
      541 gcggatttac agcgcacatt tgaggagaag atggccgcgc aggcacaagc gtttgaagag
      601 aaactggctt ccctgtctgt ggtattacgg aagtgcgtga cgggcgatga tatgcgtccg
      661 atgcttgagc agatggtgaa ggaggcggta agccatatcc ctgttccgcg tgacggtcgt
      721 gactacgatc cggaagtact gcagaaggcg gtgaatgatg cggtcgcaaa tattccacag
      781 ccggcggacg gtaaaagtct caccccggat gatgtgcgtc cgatgcttga acagatggtg
      841 aaggaggcgg taagccatat tcctgttccg cgtgatggtc gtgactacga tccggatgtt
      901 ctgcagaagg cggttctgga tgcggtgagt gccctgccgg ctccgcagga cgggcgtgat
      961 gccacggcac tggaaatact ccccgccatt gacgatcaaa aatcctttcc ccggggcacg
     1021 tatgccacac accagggcgg actctggcgg gcgtatgaaa aaacgcacgg gatgcgggga
     1081 tgggaatgcc tggttgacgg ggtggcggat attgacgtca gtatgacggg ggagcggttg
     1141 ttctctgtgg tgatccggca cagcagtggc cagcgtacgg aaaaaacatt ttccctgccg
     1201 gtgatgctct accgcggtgt gttcagagcc ggtgaaacct accaccccgg cgatacggtg
     1261 acg
//
LOCUS       DAZUFB010000342         1262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZUFB010000342 DAZUFB010000000
VERSION     DAZUFB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.352"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHY3M_005448"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHY3M_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFS4320118.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT          323 a          316 c          318 g          305 t
ORIGIN      
        1 cgtcccggcc atatctccgg tgagcggctc attccccgcg cggtttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca gaaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctcccccagt
      661 tcaaaccagg tcaccacgcc tttcacctgg cgctgtaatt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcgt ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaatct
     1081 caacctcttg tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa
     1141 acgcaaagtt aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt
     1201 aacattatta aaatgaatga ttgcataaga aatatatgct accgctccac cctgataaca
     1261 tg
//
LOCUS       DAZUFB010000343         1243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZUFB010000343 DAZUFB010000000
VERSION     DAZUFB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.353"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(330..608)
                     /locus_tag="ACHY3M_005449"
     CDS             complement(330..608)
                     /locus_tag="ACHY3M_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFS4320119.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            1036..1182
                     /locus_tag="ACHY3M_005450"
     CDS             1036..1182
                     /locus_tag="ACHY3M_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320120.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          369 a          218 c          243 g          413 t
ORIGIN      
        1 aatctattga tgttaatggt tttttattgt ttgttgttcg gctttttata ctaatttgag
       61 cgaaacaaaa agataaaaag ttgtttttcg tgtctttaag tgataccaga tggtattgcg
      121 ctaaatgtac caatgggtat accaagagca ctaaaaacaa aggggcaacc actttgtaac
      181 cccttgatta tttgcagaag cgcagaaaat cgaactctgg aaccctttcg gggcatcctt
      241 tttcaagaat gaagaaaaaa ataactaaaa aatcaagttg ttaggaagtt attcatggct
      301 agtgtaccaa ctcttttctg ggctaatagc tatgatttgt ttgtattatt tgtctctgtc
      361 tctattattt ttgacatcca tgcgacttct gatattgata atctcagttg tggagttctg
      421 tctgcgcttc cgggaggacg catatcaaaa ctacaggatc ttctgttaaa cactgtaaag
      481 ccatctacat tgcatgttgt tgagtctaag atacaatttt tagtagatga tttatctgct
      541 ataggcctag atatttcagg ttgatgccaa gtgtgtcctg aaaaaatact ttttattgaa
      601 aatggcattt ttatcctcac attatgtctt tctcttatag tgcaatttaa tgtatttaga
      661 aacattaaac tatgaatcta aaaaggctac gcagagtact gttacacttt aacaaatcat
      721 atttaccact ctggaaatgg gatggctggt tcataaaata atgagtcttt ttcaacggtt
      781 gttacattgg tgtactgatg attatgcctt gtcgctaaga gctaccataa ccgtacagcg
      841 tgattcatcc atggcgaata aatcgactga taaatccccc tgagtttcag ctcttgctgt
      901 tggaccttgc ccgtttcgct atggaacgcg taccagctag agaatattat tcgttctcct
      961 ccaatgttac gcaagttttg taaacggtaa ccaatcgtga ttagtcctgc atgtaacgga
     1021 catcaagctg tctggttgat ttttattgcc agcagttcga gagagcagac aactgcgtcc
     1081 gatggaaaaa tgggcacgac agggtattca ggcaacctcg ctgaggaggc ttatggtaca
     1141 gattgtaatg tgaagtataa aacgttaaaa attccatttt agaaactaaa tagctgtaat
     1201 ataagttcga tctaatctac tattttgaca gacagattgt atc
//
LOCUS       DAZUFB010000344         1231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZUFB010000344 DAZUFB010000000
VERSION     DAZUFB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.354"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            630..818
                     /gene="dicB"
                     /locus_tag="ACHY3M_005451"
                     /gene_synonym="ftsT"
     CDS             630..818
                     /gene="dicB"
                     /locus_tag="ACHY3M_005451"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4320121.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            815..1006
                     /gene="ydfD"
                     /locus_tag="ACHY3M_005452"
     CDS             815..1006
                     /gene="ydfD"
                     /locus_tag="ACHY3M_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFS4320122.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            1100..>1231
                     /locus_tag="ACHY3M_005453"
     CDS             1100..>1231
                     /locus_tag="ACHY3M_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4320123.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     "
BASE COUNT          338 a          261 c          287 g          345 t
ORIGIN      
        1 agcggtaaat tttgtcgaaa tcatctttcc cctctggttt attcagagga ttacaggacc
       61 cgtaagtagc ggcatacatt tcaaccgttt tatcaaaaat ataagagaca aattcttctt
      121 tagtcatata gatttccttc ttggttattc ggaataagaa ggataccacc tcgcctgacg
      181 tggttaaaag caggcacaca acacgaaagc gcacggcgaa gttcgtctca ctgtacggtg
      241 tcgttaaatt taattcgacc gtgcgcttcc ggttgtggca acccgcgaaa tggcgcggcg
      301 gtaagtatgg cggggttatt ccttccccgt tgaggacacc gggttgtcag gttgaccata
      361 cgcttaagtg acaaccccgc tgcaacgccc tctgttatca attttctggt gacgtttggc
      421 ggtatcagtt ttactccgtg actgctctgc cgccgttttt aaagtgaatt ttgtgatgcg
      481 gtgaatgcgg ctaagcgcac gcggaacagt taaaaccaaa aacagtgtta tgggtggatt
      541 ctctgtatcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc aggcactgca
      601 tcacaaaatt cattgttgag gacgcgataa tgaaaacgtt attaccaaac gttaatacgt
      661 ctgaaggttg ttttgaaatt ggtgtcacta tcagtaaccc tgtatttact gaagatgcca
      721 ttaacaagag aaaacaagaa cgggagctat taaataaaat atgcattgtt tcaatgctgg
      781 ctcgtttacg tctgatgcca aaaggatgtg cacaatgaat tcagcatttg cgcttgttct
      841 gacagttttt cttgtttccg gagagccagt tgatattgca gtcagtgttc acaggacaat
      901 gcaggagtgt atgactgcag caaccgaaca gaaaattccc ggtaactgtt acccggtcga
      961 taaagttatt caccaggata atatcgaaat cccggcaggt ctttaaaaca gttccgtaat
     1021 aaatatccgg tttcattctt atatgccagc aatggcaggg atttgttcat ccttaaatct
     1081 gtcatgaggt taaaacaaaa tgagtaaagt ctttatttgc gccgctattc ctgacgaact
     1141 ggcaacaagg gaagaaggcg ctgtggctgt agccacagcc attgaagctg gcgacgaacg
     1201 ccgtgctcga gcaaaatttc actggcagtt c
//
LOCUS       DAZUFB010000345         1226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZUFB010000345 DAZUFB010000000
VERSION     DAZUFB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.355"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..295
                     /gene="ykfM"
                     /locus_tag="ACHY3M_005454"
     CDS             <1..295
                     /gene="ykfM"
                     /locus_tag="ACHY3M_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFS4320124.1"
                     /translation="SFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLLIAIV
                     IAPLIAPFVTGCVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(432..1170)
                     /locus_tag="ACHY3M_005455"
                     /pseudo
     CDS             complement(432..1170)
                     /locus_tag="ACHY3M_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          345 a          244 c          223 g          414 t
ORIGIN      
        1 ttccttttta aatattcatg taattttact tacgataata aaatcattct taatcaaaaa
       61 aacgaaggta gtcaatttcg ccacagacat aacaatacag ctaactctta tatttttact
      121 aatagctatc gtaatagcac ctttaatagc tccttttgtc acaggatgtg tcaatgctaa
      181 ttatcacccc tgcggtaata atacggggat tttcccagga gcaatctata ttaaaaatgg
      241 catgaaatgt aataatgaat atatttctcg caaggaagat tcagcagtta aataattaaa
      301 tattctaaaa caaacagttt ttaactccgg attccatgtc ctttcttgca ctgtctttgt
      361 caagaaaaat ttcttatgca aagaattttg tattattgcc gccagaaatt cctggcggca
      421 gatataattt attattgata gcggttaacc aaatctttta ccatcgcttt ataggtcttt
      481 tgcttaagtt gcatgatttg ctgctctgtc atatcagtcg tactggcttc attcttgtcg
      541 ggcaatggtg gaagcacttt atattcataa ttcaccggcg accaggtagc gcagggagat
      601 agcgtcggtg agtttttcat ttttatagtc ggaccatact gttgatttca gcgcagagtc
      661 atattttcct gtctgcaacg tatcctggaa aacaataatc gctttctgat gcccttttgc
      721 agccacaaaa cgcagtgcct gcatccagtt catattctca gcactggcaa catcctcgtt
      781 ctcattttta tctttgtttt tgttacaagt cagaggcttt tgcctgtccg ggataccaga
      841 gaatgtggaa acagtataat attttcgcat ctcctcctgc ataatggttt ccggtgcgcg
      901 gttgccggat tgaacaagta ttacaggaga attaagggga acgcggaact catctccatc
      961 aagcgcagct tgaatatcct cttcggatac ggccagagtg gtttcattac cgaataaatc
     1021 tttgtctgtc agttgctggg gagtgttttt attaatatta ctttgtgtga cttgagcttt
     1081 tttcttcttt tcatcaatag aagattcaga ggcacatcct gcaagcagcg agaataacac
     1141 acatatgcaa caaagctttt ttgaattcat aattggacac tccctcgcct gtcataatca
     1201 tggtaatgtt tagctaacga ttttta
//
LOCUS       DAZUFB010000346         1212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZUFB010000346 DAZUFB010000000
VERSION     DAZUFB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.356"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1212
                     /locus_tag="ACHY3M_005456"
     CDS             <1..>1212
                     /locus_tag="ACHY3M_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320125.1"
                     /translation="RLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNENAAARASNEAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAESDVQRADWQKGLQE
                     GFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWAASILQEISKVLM
                     NAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPT
                     YFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDG
                     TNGQIGPEAAG"
BASE COUNT          312 a          272 c          410 g          218 t
ORIGIN      
        1 ccgactgggg tatgcggcgg atgacctcgg ttttgtgggt gatccgtatc aggaggcgag
       61 acaacgttat atcagtaatg ccctggaagc ctggcgcaat aacgaggcga ataaacccaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaaa gagcaactgg cactggccgg tcagaacacg gagctggcga agctgaagta
      241 ccagacagcg ctgggtgaac tgaaaaccct gtcggagata cagaagcagg agctgctgcg
      301 taacgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 cctgaagaat gagaatgcgg ctgcgcgtgc atcaaatgaa gccgaactgc tggggtacgg
      421 gcagggagaa cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggcg ctggcgcaga atgcacagta tctgagtgaa cgtctgaaag agcaggaagc
      601 cttttatgcc gaatcggatg tgcagcgtgc ggactggcag aaagggctgc aggagggatt
      661 cagtaactgg gtggataatg cgtccgatta cgcctcacag gcagcacagc ttgcgacgga
      721 gggtatctca gggatggtga ataacatcac ggagatgctg aacggaaata aagtggaatg
      781 gcgcagctgg gctgcatcaa tcctgcagga aatatcaaaa gttcttatga atgccgcgat
      841 tgtcaacgga attaagacgg cggcaaacgg tatgtccggt gcgggaggat ttctcggcag
      901 cattggtgac tggctgggcg gggcggtggc caatgcaaaa ggcggcgtgt atacctcggc
      961 aaacctgagt gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa
     1021 aggggccggg ctgatggggg aggccggacc tgaagccatt atgcctctga cgcgggcggc
     1081 ggatggctcg ctgggtgtgc gagcggtggg cagtatgaac ggtagtgcgg gtctggtgta
     1141 ttccccggtc taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga
     1201 agctgcgggc ag
//
LOCUS       DAZUFB010000347         1212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZUFB010000347 DAZUFB010000000
VERSION     DAZUFB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.357"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1212
                     /locus_tag="ACHY3M_005457"
     CDS             <1..>1212
                     /locus_tag="ACHY3M_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320126.1"
                     /translation="RLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKAQLALAGQNTELAKLKYQTAQGELKTLTEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQETFYAESDAQRADWQKGLQE
                     GFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLM
                     NAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPT
                     YFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDG
                     TNGQIGPEAAG"
BASE COUNT          307 a          276 c          411 g          218 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgaccttgg ttttgtgggg gattcgtatc aggaggcgag
       61 acagcgttat atcagtaatg ctctggaagc ctggcgtaat aacgaggcga acaaacctaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaag gcgcaactgg cactggccgg tcagaataca gagctggcga agctgaagta
      241 ccagactgcg cagggcgaac tgaaaaccct gacggagata cagaagcagg agctgctgcg
      301 caatgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 actgaagaat gagaatgcgg ctgcgcgtgc gtcgaatgat gctgaactgc tggggtacgg
      421 gcagggggag cgagccagag aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggct ctggcacaga acgcgcagta tctgagtgaa cgccttaaag accaggaaac
      601 tttttatgcc gaatcggatg cgcagcgtgc ggactggcag aaagggctgc aggagggatt
      661 cagtaactgg gtggataatg cgtccgatta cgcctcacag gcagcacagc ttgcgacgga
      721 gggtatctca gggatggtga ataacatcac ggagatgctg aacggaaaca aagtggaatg
      781 gcgcagctgg gcctcatccg tactgcagga aatatcaaaa gttcttatga atgcggccat
      841 tgtcaacgga attaagacgg cggcaaacgg tatgtccggt gcgggaggat ttctcggcag
      901 cattggtgac tggctgggcg gagcggtggc caatgcaaaa ggcggcgtgt atacctcggc
      961 aaacctgagt gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa
     1021 aggggccggg ctgatggggg aggccggacc tgaagccatt atgcccctga cccgggcggc
     1081 ggatggctcg ctgggtgtgc gcgcggtggg cagtatgaac ggcagtgcgg gtctggtgta
     1141 ttccccggtc taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga
     1201 ggcggcaggc ag
//
LOCUS       DAZUFB010000348         1208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZUFB010000348 DAZUFB010000000
VERSION     DAZUFB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.358"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            116..>1208
                     /gene="gmd"
                     /locus_tag="ACHY3M_005458"
     CDS             116..>1208
                     /gene="gmd"
                     /locus_tag="ACHY3M_005458"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFS4320127.1"
                     /translation="MNKVALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDSHLANPKLFLHYGDLTDTSNLTRILKEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRILGLEKKTKFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMFACNGILFNHESPRRGETFVTRKITRGIANIAQGLEKCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQETPEDFVIATGIQYSVREFVTMAAEQVGIELAF
                     EGEGVNEKGVVVSVNGTDAKAVNPGDVIISVDPRYFRPAEVETLLGDPTNAHKKLGWS
                     PEITLREMVKEMVSSDLAIAKKNVLLKANN"
BASE COUNT          384 a          197 c          262 g          365 t
ORIGIN      
        1 actttgatgc ttttgaaaaa aataatagac tagcatcaat aataaaatca aataatgatt
       61 tttgacttga gcagaaatta tttatatttc aatctgaaaa ataaaggctg ttattatgaa
      121 taaagtggca ttaattactg gtatcactgg gcaagatggc tcctatttgg cagaattatt
      181 gttagaaaaa ggttatgaag ttcatggtat taaacgccgt gcatcttcat ttaatactga
      241 gcgagtggat cacatctatc aggattcaca tttagctaat cctaaacttt ttctacacta
      301 tggcgatttg acagatactt ccaatctgac ccgtatttta aaagaagttc aaccagatga
      361 agtttacaat ttgggggcga tgagccatgt agcggtatca tttgagtcac cagaatacac
      421 tgctgatgtt gatgcgatag gaacattgcg tcttcttgaa gctatcagga tattggggct
      481 ggaaaaaaag acaaaatttt atcaggcttc aacttcagag ctttatggtt tggttcaaga
      541 aattccacaa aaagagacta cgccatttta tccacgttcg ccttatgctg ttgcaaaatt
      601 atatgcctat tggatcactg ttaattatcg tgagtcttat ggtatgtttg cctgcaatgg
      661 tattctcttt aaccacgaat cacctcgccg tggcgagacc tttgttactc gtaaaataac
      721 acgcgggata gcaaatattg ctcaaggtct tgagaaatgc ttatacttgg gaaatatgga
      781 ttctctgcgt gattggggac atgctaagga ttatgtcaaa atgcaatgga tgatgctgca
      841 gcaagaaact ccagaagatt ttgtaattgc tacaggaatt caatattctg tccgtgagtt
      901 tgtcacaatg gcggcagagc aagtaggcat agagttagca tttgaaggtg agggagtaaa
      961 tgaaaaaggt gttgttgttt cggtcaatgg cactgatgct aaagctgtaa acccgggcga
     1021 tgtaattata tctgtagatc caaggtattt taggcctgca gaagttgaaa ccttgcttgg
     1081 cgatcctact aatgcgcata aaaaattagg atggagccct gaaattacat tgcgtgaaat
     1141 ggtaaaagaa atggtttcca gcgatttagc aatagcgaaa aagaacgtct tgctgaaagc
     1201 taataaca
//
LOCUS       DAZUFB010000349         1205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZUFB010000349 DAZUFB010000000
VERSION     DAZUFB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.359"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..494
                     /locus_tag="ACHY3M_005459"
     CDS             3..494
                     /locus_tag="ACHY3M_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="simA domain protein"
                     /protein_id="HFS4320128.1"
                     /translation="MRKLLLPLLFMAGTVNAASSVKEICTDYTKYLGHVYGFAVSQDE
                     SMRKKLLSDMKRLKLSEAMVQQELYKVATNENAKYQYSRLLNPDANEINRSSFDYMVK
                     ACETAPDFAIPSWGVLVASNAVNKEDVGRNGIDSIRNAPGMRHQNVQGTLEERARGPG
                     VAP"
     gene            583..1089
                     /locus_tag="ACHY3M_005460"
     CDS             583..1089
                     /locus_tag="ACHY3M_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320129.1"
                     /translation="MKLYKSLYSFLLISSFLPLSAMAGSTAWTVGGEQGWREISATND
                     DGYTINFSCDAGAREGSEDHIAGRNLYVSGGKENADFSTRDTISRKADVITLIVGPDS
                     FNIGTQNTAPNRREWYSFWKSASATKEKNMDVYIGSRRITSFSLDGISSIYKEAKNDG
                     CLKQDDGE"
BASE COUNT          397 a          210 c          271 g          327 t
ORIGIN      
        1 taatgagaaa actattacta ccgttattat ttatggctgg gactgttaat gcagcatcaa
       61 gcgtaaagga gatttgtacc gattatacga aataccttgg gcacgtttac ggctttgctg
      121 tcagtcaaga cgaatccatg cgcaagaagt tactgtcaga tatgaaacgc cttaaacttt
      181 ctgaagcgat ggtgcagcaa gaactgtata aagtcgcaac caacgaaaat gctaaatatc
      241 aatattctcg cctattaaat cccgacgcaa acgagatcaa tcgaagctct ttcgattata
      301 tggtaaaggc atgcgaaacc gctcctgatt ttgctatccc tagctggggg gtgctggtgg
      361 cgagcaatgc cgttaataaa gaagatgttg gaagaaatgg cattgactca atcagaaatg
      421 ccccaggaat gcgccatcaa aacgtgcagg gtacgcttga agagcgggcc agggggccgg
      481 gtgtagctcc ataatttaat gaataatatt aaattctctg ggcatagtgg acctaacaat
      541 atggactatg cctataatat ctaaaaccaa aaggataaaa atatgaaatt gtataagtca
      601 ttgtatagct ttttattaat atcctctttt ctgccattat cagcaatggc aggttctacc
      661 gcttggacgg taggaggtga gcaaggatgg agagaaatct ctgcaaccaa tgacgatggt
      721 tatacaatta acttttcttg tgatgctgga gcaagggaag gttccgaaga tcatatcgcc
      781 ggaagaaatt tgtatgtaag cggagggaaa gagaatgctg atttttctac acgcgacaca
      841 atttctcgta aagctgatgt aattacctta attgttggtc cggatagctt taatattggc
      901 acgcaaaaca ctgctccaaa tcgtagggaa tggtattctt tttggaagtc agcatcagcc
      961 acaaaagaaa aaaacatgga tgtatatatc ggatcgcgtc gaattacatc attctctctt
     1021 gatggaattt caagtattta caaggaagct aaaaatgatg ggtgtttaaa gcaagatgac
     1081 ggtgaataaa gtgaaggata ttaaaaaact tgcacttggc tcagtaagcg atctctataa
     1141 tagagattca aatattactt ggcgagataa tttaagtaaa atcaaaatgc caggatatac
     1201 atcgg
//
LOCUS       DAZUFB010000350         1185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZUFB010000350 DAZUFB010000000
VERSION     DAZUFB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.360"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..494
                     /locus_tag="ACHY3M_005461"
     CDS             <1..494
                     /locus_tag="ACHY3M_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4320130.1"
                     /translation="AAGYSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQ
                     KKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYMEDGLPDPVKAMGRLLVEN
                     INTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNE
                     GNG"
     gene            491..>1185
                     /locus_tag="ACHY3M_005462"
     CDS             491..>1185
                     /locus_tag="ACHY3M_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4320131.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVF"
BASE COUNT          270 a          303 c          374 g          238 t
ORIGIN      
        1 ttgccgctgg ttattcggcg aagaccgcca gggttaaagg ctcgcagctg gcaaaagatc
       61 cggaggtgct tgcgtttata gcccgtaaac aatgcgagac ggtggaggtg gatgaggttc
      121 ctgtttaccg gcagaaaaaa tcagagcagg aggataaacc ccgtcgccgt gaggcggctg
      181 caataccaca gccggacgaa aacaatccgg agatgccacc gtccgcggtg atgtctcctg
      241 gtattgaata tatggaggat ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg
      301 tggagaacat taataccgac cccaggctgg cgctggatgc ggcttataag ctggcgcagt
      361 tcacgcacca taaaaaaggg gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa
      421 aagcggctaa ccgttttgcg gtgccaccac caccccgcct ggtggtgaat aatgataatg
      481 agggcaacgg atgatacctg tgtggagcac ggcctgcccg gactgggcag agcgcctgaa
      541 aaaggggctg tcgattattc cggctccgat ttatccggac caggctgcac atgcactggc
      601 gatttttaaa caactgcgaa ttgtggatgc accgggtagc ccgacattcg gggagtcctg
      661 tgcaccgtgg gtgtttgacc tggtggcggc cctgtttggc tcctacgatg cgcagaccgg
      721 tgttcgccat atcaaggaag tgtttatcct tatccccaag aaaaactcga agtccacgct
      781 ggctgcgggg atcatgatga cggcgctgtt actgaactgg cggcaggcgg cgggctacac
      841 cattctggcc ccgaccgtgg aggtggcggc taacgccttc aaccctgcca gggatatggt
      901 acgacgggac gatgatctgg atgacctctg tcaggtgcag acacatatcc ggaccatcac
      961 ccatcgggtg acggacacca ccctgaaggt ggtggcagcc gatccgaata cggtgtccgg
     1021 tatcaagtcc gtggggactc tgattgatga actgtggctg tttggcaagc agtacaaggc
     1081 ggaagacatg ctacgtgaag ccatcggcgg gcttgcctcc cgtccggaag ggtttgtggt
     1141 gtatacgacc acccagtcga atgaaccgcc cgccggggtg ttcag
//
LOCUS       DAZUFB010000351         1174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZUFB010000351 DAZUFB010000000
VERSION     DAZUFB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.361"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..575)
                     /locus_tag="ACHY3M_005463"
                     /pseudo
     CDS             complement(1..575)
                     /locus_tag="ACHY3M_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            833..>1174
                     /locus_tag="ACHY3M_005464"
     CDS             833..>1174
                     /locus_tag="ACHY3M_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS4320132.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTYFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQ"
BASE COUNT          403 a          218 c          165 g          388 t
ORIGIN      
        1 accatataaa acttcataat tcctgttttt gtttacaaga attttatcga tatgtctcag
       61 ctgcggatca ttactggcat taacaatata aaaagcaatg aaaatgatag caaaaagaaa
      121 aaaagcatta catacttaaa cagcttactg ctaaagaaat gttgttttgt agaaataatg
      181 ttattttcaa taatgcctgg caatgaatac tgccaaactt catttttttc ttttacagca
      241 aatttgagac ctaaaacttc tatttgctga ttcaatgtta acgttctatc ttcaggttct
      301 tgtgctgtta attctctgaa tgttactacc agatcagggt cggtagtaat tattatctca
      361 aaactacctg taagctcgcc ataaggaacg actattgtgt tttctggtaa ctcagaaaaa
      421 gcatcacttc ttcctaacga aacatcatta ccaatgataa caagtagacg cccattaagt
      481 atttcatact cgcatccatt caatggtccg ctcaacaaac gaatggcata agattccaat
      541 aagtcttgtg ataagaattt atcattattt tccatgaata ccacccgcta attccataag
      601 ctctagttta gagtagtgta acaagacgga aatttcatga ccaaattatg atgacacaga
      661 ataaaaaaat tataattccg agaactattt taaatgcctc tatgcataac tttatttaca
      721 atttaaggta taaaccctat tccctcaatg ttaagcaaat ataacaatta ctcttatttt
      781 aaattttaca cgtaatattt accctaaatg attttagagt acaggtgggc atatgcaagt
      841 cttttccagt gatgtctact tcactgtagg cactaatgca ttattagcat cccaaaaaga
      901 atattacagc gatttagttg cgttggttga tcttggacat tcgttcgttg ttattgatga
      961 gcatcaacat cgaaatctca aaccaaatac agaacctgta aacatccttt tgagcaacaa
     1021 tttcatcagg atcaataaga atattacatt atctgattta acgtatttcc tggtttcaaa
     1081 tcttcataca caaaatgtgt attccacaca agaaccactt acccatgatg agatagatat
     1141 cctgcggtta tgtgtttctt actcgttaaa acaa
//
LOCUS       DAZUFB010000352         1171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZUFB010000352 DAZUFB010000000
VERSION     DAZUFB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.362"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..335
                     /locus_tag="ACHY3M_005465"
     CDS             114..335
                     /locus_tag="ACHY3M_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4320133.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAKAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            415..709
                     /locus_tag="ACHY3M_005466"
                     /pseudo
     CDS             415..709
                     /locus_tag="ACHY3M_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            912..1171
                     /locus_tag="ACHY3M_005467"
                     /pseudo
     CDS             912..1171
                     /locus_tag="ACHY3M_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248356.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
BASE COUNT          297 a          320 c          270 g          284 t
ORIGIN      
        1 ggtggcaacc aggttttctc ctttgccgaa catggtctgc tttaacccgt caccgtcacc
       61 accacatcac acctgttttc acttttatct tctgtcttca gaggtatcac accatgaaaa
      121 tcatcacccg tggtgaagcc atgcgtattc accaacagca tcctgcatcc cgtctttttc
      181 cgttctgtac cggcaaatac cgctggcacg gcagtgctaa ggcgtatacc ggtcgtgaag
      241 tgcagaatat tcccggtgtg ctggccgtgt ttgcagaacg ccgtaaggac agttttggcc
      301 cgtatgtccg gctgatgagc gtcactctga actgagtcag taagggcatt ctgatgagca
      361 gaattatcga cattacctga ccgttcttaa ccgattttct gtgaggattt tatcgtgtca
      421 gacacgctcc atgaaacaaa ttatcccgac gacaatcacg accgcccctg gtgggggctg
      481 ccctgcaccg ttcgtgacag tgacaccccg ttttggcgcc cgtctggtgc aggagggcaa
      541 tcagttgcat taccttgcag accgcgccgg tatcagaggc cggttcagcg atgcggatgc
      601 ataccatctg gaccaagcct ttccactgct gatgaaacaa ctggaactca tgttcaccag
      661 cggtgaactg aatccccgtc atcagcagac cgtcacgctg tatgcgaaaa ggatgatcgg
      721 tgaatccccc cttcggttcg cactggtaca tttgccagcg atgaaaaagc taggactgca
      781 ttatgccata ccgaccgaca taacaatacg gagcagggca taaaccaaga tacgctggct
      841 gccatacgac agtgacgtac cgcgaaaaac ccgtccgcaa ttaatttctt ttattcgcag
      901 aataaccgac gatgagaatt ttacctatat tccgaaaacg tgtggcgaag tcctctgtaa
      961 ccgtctgaca gcccctagtt acatacctgc aggacaaaca ctacagcctg actcttaacg
     1021 atacgcagcg cattgacgac actgttatga aggtacatat tgaaaacggg attcccctta
     1081 gccgatacag tcaacttcct agtagataag tacgaactga ttcgtatcga tcacagagtg
     1141 ttgagcaggc gggagcaatc acattatctt c
//
LOCUS       DAZUFB010000353         1154 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZUFB010000353 DAZUFB010000000
VERSION     DAZUFB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.363"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..619
                     /locus_tag="ACHY3M_005468"
     CDS             <1..619
                     /locus_tag="ACHY3M_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFS4320134.1"
                     /translation="ASGGLGKIPQDTLSGIRTLAFNGGAQVQMGSTIVTIRSVFLGFF
                     MGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASPPRPSVMQTEL
                     VLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVCTLYDKESLKH
                     LVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(879..1098)
                     /locus_tag="ACHY3M_005469"
                     /pseudo
     CDS             complement(879..1098)
                     /locus_tag="ACHY3M_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          333 a          207 c          271 g          343 t
ORIGIN      
        1 agcgagtggg ggactgggga aaataccgca ggatactctt tctggcatac gaacactggc
       61 ttttaacgga ggagctcagg ttcagatggg gagcactatt gttacaatac gttctgtctt
      121 tttaggcttc tttatgggaa gtgtaagccc tgaaggatta tcagagagag ctttacgaac
      181 tgcattgaat aatgttaatc gtcttgaacg cgaccttaat ggagggctat ccggccgcca
      241 gattttagca agtataccca gcccgtatgc ttctccacct cgtccttctg taatgcaaac
      301 agagttggtg ttagagcaaa tagaaaaatg ctcatttaat gttaactcgt catctctgag
      361 ggcgactgaa gaggcattaa catgcccgat tacattatgc atccctgagc atggtgtgat
      421 tatgagaaat gctggagatt cagatgtatg tactttatat gataaagaat cattaaaaca
      481 tcttgttaac acaagttcac ctcatcctct gagcagagaa aaaataacag agtcaatgat
      541 tgttaaggaa aactcgtgct attttgattt cacatcagga agtattaaaa atgttcgtgg
      601 tgagataact agactataag atgttagaac atattgactg ataagtgtaa ttcacagaca
      661 tcttttcagt tttttctgtt aattttctaa ttgatgggct gtatacaggg ataaaaggag
      721 agggtattgt ctggacattt ctgttagcat aattggaaat gtgcgtgaaa aaaagcataa
      781 cagacatcat gtgttatgta tgtattcaga gtgcccataa ttcaccagtg cagggataaa
      841 ttgtaaaaaa atcgcagtac gtcgtatgca gaacggtgcg gcgtacgcag ttccagtgga
      901 ccgtcgcctg tgataacggt ctttgtggaa taaccgttgc gggagttagc tcctgatctg
      961 gactgatttt tctcattccc aagatggtgt gtcatctctg cattgagagc ggcttcaacg
     1021 ctgagctttt tcagcaaccg atcaaactga ctgaggtctt ctgagggggc tgaggttttt
     1081 ggccagttcg ttagcaatac accagaaaaa ttctaaacga gcctgtacat agatttgtgt
     1141 aattgcctga tttt
//
LOCUS       DAZUFB010000354         1131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZUFB010000354 DAZUFB010000000
VERSION     DAZUFB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.364"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            426..1131
                     /locus_tag="ACHY3M_005470"
                     /pseudo
     CDS             426..1131
                     /locus_tag="ACHY3M_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          418 a          219 c          165 g          329 t
ORIGIN      
        1 accacgtaat catgcttctc tgacatttta tcggagaagc aatgactctt tgtctcagta
       61 ccctcctcct ttttaaaagg cagatttcag tcgctaatac aacacatttt taaatacact
      121 ttaacctggt tcattattac acatctaaaa catcacaaaa atattacttc acacactatc
      181 ggagaacaaa ataacttttt aaaatgagcg ctttttgata gttgttaacg tttcccttta
      241 cgatactatc actgcctttt tttatacaca aatattaccg ggtggaaatg aaagggaatt
      301 taaaggtaat tatatcagga agatataacg cattatattt attgtgtgta gaagaaaatc
      361 cattcctgtt ctaattatcc aatttaaaca tcttagcata aaacaaatga tgaataagga
      421 attttatgtc aattgacttt accccaggta taataaatac atatcacggc gatatttata
      481 actgcacaac aaataccgat aatgctaaaa cgccagacac accgaagtgg ccttgcgata
      541 attgggaaga acaacaaccc attaattcca cattctctgg agaaggatat atctctgatc
      601 aatatgattt agcgcaacac caacttcaac aaattaatgc gtgccacacg aacactacat
      661 atacaaatgc agactactca aaagttgtgg ctcaacttgt aagtcttatt actaacattg
      721 aaacgataag ttcgacataa cttacgcaac agacacaatc aatacttaat cagattaata
      781 acattagata tgaaaaaaac aaaagtgctg aatgtcgaat cattgttgtc gctaatccta
      841 aacccgacaa ggcaatcata actaaaatta gcgtagaaga gggaataccc ataacattca
      901 gtgtccaaac catgttttca gatactaatt ttattgctga acaacgagct gacttaccaa
      961 ctaatataaa agatattcaa agcctgtatc aaaaaatgac gaagttatat attgagcaca
     1021 gtgaaaataa aaacaggatg aaagtctttg ccggaaccaa ctttattgat ttcaatatga
     1081 caggacagaa cctgtccggg ttcgttctaa ccttgtcaag gttttatttc g
//
LOCUS       DAZUFB010000355         1130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZUFB010000355 DAZUFB010000000
VERSION     DAZUFB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.365"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(227..916)
                     /locus_tag="ACHY3M_005471"
     CDS             complement(227..916)
                     /locus_tag="ACHY3M_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4320135.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(931..1053)
                     /locus_tag="ACHY3M_005472"
     CDS             complement(931..1053)
                     /locus_tag="ACHY3M_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFS4320136.1"
                     /translation="MMSESYISELLRCRWGLVCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          313 a          292 c          205 g          320 t
ORIGIN      
        1 cgcaccatcc ttcataacca ccccaataca agatgataac attggcttac aactcataac
       61 aaaagcaatt caatgccgtc aagaggttac aggctaaaaa aactctatta catagcagcc
      121 agcatgttta ccgtacaagt acaactcagg gcataaaaaa aacccactcg gcagcgggtt
      181 tatacatttt ttacaacata ccaaatttgc atgaagtata tggcttttaa tccagttttg
      241 caatattttg ctgtaaaaat gctgcctttt gttttgaacg tgttctcgtc acaagcaata
      301 aagcatcact atcaagctgt agaaaaatgt gcttcattgc aacccagcgt tcagtaaatg
      361 tctcggacca gttttttgtt gtcactccca ccaatgatgc cagtgtctgg tattcatagg
      421 cctcacgccc tgcaagttcg ctcttcacat cctgtgcagc cagccagatt aacgtcttca
      481 ggcgatccag tgtcttacct gcaatttttc tgttacttaa caaatcttta aactcgctcc
      541 atgcccattg cgttatggtg acctgatgcc cccatcgaac gctttcgctg taacaccaaa
      601 gcaaccatgc tttctgatgt tcatcgagag acaaaaccgc gcggcgccat gaagaggttg
      661 agaattcaac cgggctgacc aaagcaatgg atgaaccttt tgcgtacgac tgcttaccgg
      721 aagtcggcgt attatccagc gtaatcatct tgccagttac cacatccaga atgcgcggct
      781 tctttcgttt gtatgtacca gtatcaaatt gtgcatgctc ctgccaggct tcgagctggc
      841 ctttcgttgc tccgttcaag tcagcagtag ctgccataag ttgctcacga acatactgta
      901 aatattgggt attcatgcag taaatccttt ctatattttg gcataattct tcaacattcg
      961 gtaatcgttc aaaaccgaat cggggaaacg acataagcac acgagccccc agcgacagcg
     1021 aaggagttct gatatataag actcagacat cattcattcc ccggttctcc aatatctgtt
     1081 tcactcatca tccataacta cctgtaattg cccccccctt tttttgtaac
//
LOCUS       DAZUFB010000356         1127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZUFB010000356 DAZUFB010000000
VERSION     DAZUFB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.366"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..735)
                     /locus_tag="ACHY3M_005473"
     CDS             complement(<1..735)
                     /locus_tag="ACHY3M_005473"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4320137.1"
                     /translation="MDLQKLDKYTCNGNLDAPLVSIIIATYNSELDIAKCLQSVTNQS
                     YKNIEIIIMDGGSSDKTLDIAKSFKDDRIKIVSEKDRGIYDAWNKAVDLSIGDWVAFI
                     GSDDVYYHTDAIASLMKGVMVSNGAPVVYGRTAHEGPDRNISGFSGSEWYNLTGFKFN
                     YYKCNLPLPIMSAIYSRDFFRNERFDIKLKIVADADWFLRCFIKWSKEKSPYFINDTT
                     PIVRMGYGGVSTDISSQVKTTLESFIV"
     gene            complement(713..>1127)
                     /gene="wzy"
                     /locus_tag="ACHY3M_005474"
     CDS             complement(713..>1127)
                     /gene="wzy"
                     /locus_tag="ACHY3M_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O111 family O-antigen polymerase"
                     /protein_id="HFS4320138.1"
                     /translation="EPTASNIERTGMIYYLVSQLGDYIFHGMGTLNFLNNGGQYKTLY
                     GLPSLIPNDPHDFLLRFFISIGVIGALVYHSIFFVFFRRISFLLYERNAPFIVVSCLL
                     LLQVVLIYTLNPFDAFNRLICGLTVGVVYGFAKIR"
BASE COUNT          409 a          225 c          153 g          340 t
ORIGIN      
        1 tacaatgaaa ctttctagcg tagttttaac ttgagaagaa atatcagtcg aaaccccacc
       61 atatcccatt ctaacaatag gggtcgtgtc attaataaaa taaggtgact tctctttact
      121 ccatttgatg aaacatctca gaaaccaatc agcgtcagca acaattttta atttaatatc
      181 aaaacgttcg tttctgaaga aatcacgaga atatattgcg ctcataatgg gcaatggtaa
      241 attacatttg taataattaa acttaaatcc tgttaggttg taccattcac tgcctgaaaa
      301 tccagatatg ttcctatcgg gaccttcgtg cgctgtcctc ccataaacca caggggcgcc
      361 attagatacc ataaccccct tcatcaatga agcaattgca tctgtatgat agtaaacatc
      421 atctgaacca ataaatgcta cccaatcacc aatggataaa tcaactgctt tattccaggc
      481 atcataaatt ccacgatctt tctctgaaac tatttttatt cggtcgtctt taaacgattt
      541 tgcaatatca agcgttttat cagaagatcc tccatccatt attatgattt caatattctt
      601 ataagattga ttagttaccg attgcaaaca cttagctata tcaagttcag aattataagt
      661 tgcaatgatt attgaaacaa gtggagcgtc taaatttcca ttacaggtat acttatctaa
      721 tttttgcaaa tccataaaca actccaactg taagcccgca aatcaatcga ttaaaagcat
      781 caaaagggtt taatgtataa attaacacaa cttgtaacag taacaaacaa cttacaacaa
      841 tgaaaggagc atttctctca tataataaga aagatattct cctaaaaaaa acaaaaaata
      901 tagaatgata aaccaatgct cctatcacac caatacttat aaagaaccgt aataaaaaat
      961 catgagggtc attaggaatt aatgatggaa gtccatataa cgtcttatat tgtccgccgt
     1021 tatttaagaa atttaatgtc cccataccat ggaatatata atcaccaagc tgtgaaacca
     1081 aataatatat catccccgta cgttcaatat tacttgctgt aggttca
//
LOCUS       DAZUFB010000357         1126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZUFB010000357 DAZUFB010000000
VERSION     DAZUFB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.367"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..285)
                     /locus_tag="ACHY3M_005475"
                     /pseudo
     CDS             complement(73..285)
                     /locus_tag="ACHY3M_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            435..1103
                     /locus_tag="ACHY3M_005476"
     CDS             435..1103
                     /locus_tag="ACHY3M_005476"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4320139.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          345 a          244 c          213 g          324 t
ORIGIN      
        1 tcgccgaaaa ctgatattac tttaatcaaa agttgacgtg ccagattcga agtaaattta
       61 taaaatatca aatcaatata cattttgcac cttcgagata gtaatgactg ttactgtaac
      121 atgagttaca aacagcttca tacccagagc tactgatgta tcaactcgat tcaacaatac
      181 ccgatattta ttccatgcct ccagcaacga tctttcttcc tccgttgcga tttccagatc
      241 tacagcatcc tgcagtggcg caatatactc actgaattcc tggatgtaga actgtgtggt
      301 gacggtcttc cagccattcg gctcctgttg tatcgaagca taccaggcta tttcaatatc
      361 gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat ttaattcaca
      421 aataaaacta taacatggtg aaattaatga aaaaaaacac agatgatggg gctaaaattt
      481 acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca aatcggcttg
      541 catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat ttaggtaaca
      601 accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct gagagtagtc
      661 tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca agggctgggg
      721 aatcaaaaat taaacataaa attagccatg atgtttttga accttatccc gcggcgttac
      781 atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct ggaaatatat
      841 ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgacgat ggaactctat
      901 acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa aaactgatgc
      961 gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa ggcttaacac
     1021 atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt
     1081 tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttg
//
LOCUS       DAZUFB010000358         1124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZUFB010000358 DAZUFB010000000
VERSION     DAZUFB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.368"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..312
                     /locus_tag="ACHY3M_005477"
     CDS             <1..312
                     /locus_tag="ACHY3M_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320140.1"
                     /translation="LSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSFTSA
                     HTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAMARI
                     K"
     gene            652..>1124
                     /locus_tag="ACHY3M_005478"
     CDS             652..>1124
                     /locus_tag="ACHY3M_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS4320141.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGV"
BASE COUNT          282 a          271 c          300 g          271 t
ORIGIN      
        1 ctgagtgacg gttctttatg ggatcgccag gcttttccgg acaccacaat ccttgagatc
       61 cgtccccgga aaaggctgaa atatgcaggt gatggtggca acagcggcgg tctgctcagt
      121 tttacatcgg cacataccga cgcctggcgt cagcagggct atgaagacac gatgctggcg
      181 atggagcata tccggaaacc gctggcagca cgtcaggcac tgacccggtc agaggcggta
      241 ttgcagaaaa gcctggatat aacggaagag gcagatttgg cactgagaaa cgcgatggcc
      301 cggattaaat aaagacgccc caaagaaacg ccaccgcctg tggtggtgtt tttcctgtct
      361 ggtgttattg aaaatatttt cagatatata ttatctgggt gatggttaag atgaatttat
      421 ttaattttgg gggaagatac aaaaacaacc agtgaacata tttccggcgg gttatccgca
      481 atgctgaagg tcagttgcac tggcgatgcc ggaatacagc ttaatacgtg gttcgccagt
      541 gaagggcttc tcagacaata aacctcattc atttcaccca tcaggccgcg tcttctccgg
      601 gagacgcggc tttttcattt atatcgctta atcattcata aggagcaaat catgagatta
      661 gccagtcgtt ttggttatgc gaaccagata cgtcgtgatc gtccgctgac acacgaagaa
      721 ctgatgcgcc atgtaccgag tatttttggg gaaaaccgac atacctctcg cagtgaacac
      781 tatgcgtaca ttcccactat caccgtcctg gaaaatctgc agcaggaagg ctttcagccg
      841 ttctttgcct gccagacccg cgtgcgcgac cagagccgcc gggaatacac aaaacacatg
      901 ctgcgtctgc ggcgggccgg acagataacc ggtcagcatg tccctgaaat tattctgctc
      961 aactcccatg acggttcatc cagctaccag atgttacccg gatattttcg tgccatttgt
     1021 accaatggac tggtctgcgg tcagtctctg ggagaattgc gtgttccaca ccggggaaat
     1081 gtggtggaca gagtcattga aggggcttac gaggtggtgg gcgt
//
LOCUS       DAZUFB010000359         1107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZUFB010000359 DAZUFB010000000
VERSION     DAZUFB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.370"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..570)
                     /locus_tag="ACHY3M_005479"
     CDS             complement(<1..570)
                     /locus_tag="ACHY3M_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4320142.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLYIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSAD"
     gene            839..955
                     /locus_tag="ACHY3M_005480"
                     /pseudo
     CDS             839..955
                     /locus_tag="ACHY3M_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          262 a          309 c          287 g          249 t
ORIGIN      
        1 gtccgcactg aatgtcccct cagcgccctg ggtgaatgca gaaccaccac gacagcatgg
       61 taccagcagg atccccgcgt tattcgggat atacggaagc agttttttgg caatatataa
      121 gccctggccg acacagccgt actgcccttt gctcaggtct gccttcggat gattcagcgt
      181 actcatatcc tgcacatcat gcaggcagtg gtcagccgga atgatgtcgt tatacgtaca
      241 actctctcca cccggagtta ccgtgctgcg gcgcgccagc tgtttaatgc gcggatccgg
      301 agcatcgtaa gaatccggta acggaagccc ttcaccgtag gccatgccgt tggactgtcc
      361 ggcaagcaca accacgtaga accagtccgg ctcagatgaa gggccgacct gtggatctcc
      421 ttcaatagcc accgcctgca tcagtgtgta cggcgtaatg gcaaccggtc cgccgtatgg
      481 ctgccagccc tctttcagtt tgtgtgtcag cttttccgca aggtctgacg gcgacgccgc
      541 cctgacaaca tcatagtgtt taaatgccat gaatcctccc ggccgggata atattgtgag
      601 taaaatgagg agcgggctga agtccggaag ttacaggaca atggcagaag agagacaaca
      661 gcccgcaata cgaaaaaggc cgcgctattg cgcagagtga ttactgtcgg atattattcg
      721 ccagctgaaa tattacttca cgttttgttg tttattcctt gccgcccgcg tctccctgcg
      781 cgggcttttt ttgtccataa gaaagcccct ccggagaggg gctggagagt ggcgctatgt
      841 gccattgcat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
      901 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
      961 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gagacattta
     1021 aaaaaaaggc ccgcaaaagc gagccaggga aaataagtgt ggcgcgttgt actggattcg
     1081 aaccagtgac cgattgctta gaaggca
//
LOCUS       DAZUFB010000360         1104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZUFB010000360 DAZUFB010000000
VERSION     DAZUFB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.371"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..952)
                     /locus_tag="ACHY3M_005481"
     CDS             complement(137..952)
                     /locus_tag="ACHY3M_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFS4320143.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          323 a          254 c          229 g          298 t
ORIGIN      
        1 atttatgagt aaaggattat gtccacgata agcacctggg tcgattcctg ggaggcggcc
       61 atgagggtag ggaagcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
      121 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      181 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      241 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      301 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      361 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
      421 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
      481 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
      541 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      601 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
      661 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      721 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      781 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
      841 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
      901 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
      961 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1021 gtgcaatgta acatcagagg gcactgttgc aaatagtcgg tggtgataaa cttatcatcc
     1081 ccttttgctg atggagctgc acat
//
LOCUS       DAZUFB010000361         1104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZUFB010000361 DAZUFB010000000
VERSION     DAZUFB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.372"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHY3M_005482"
     CDS             complement(<1..191)
                     /locus_tag="ACHY3M_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone"
                     /protein_id="HFS4320144.1"
                     /translation="MNEQFCKDLAHTFGITLEEQTDALAFHDNDGHEWILECAPQSEI
                     LFFYCYLSNNEPDQINKIL"
     gene            456..1067
                     /locus_tag="ACHY3M_005483"
     CDS             456..1067
                     /locus_tag="ACHY3M_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFS4320145.1"
                     /translation="MFSPTAMVGRALAQAVTQTLRPAVTKAATQAGMGANGMRFAAMQ
                     SNLMINHGKLTNQLLQAVAKQTGSSDTQQWFKQEQITFLSRTVNKTVDDYCMSNNSVI
                     NKETKFRIFKAVESAIQQPLDMNCAQSSIGHFLQSNKYFNQKVDEQCGKGVDPITRFN
                     TQTKMIELVSREIFEQNFNTAKVSDIKALTQRAIAENVQDTRL"
BASE COUNT          344 a          185 c          239 g          336 t
ORIGIN      
        1 tttaatattt tattgatttg atctggttcg ttgtttgata ggtaacaata aaaaaacaaa
       61 atttctgatt gaggggcgca ctcgagtatc cactcatgac catcgttatc gtgaaaagcg
      121 agggcatcag tttgctcttc cagtgttatc ccaaacgtat gtgcaagatc tttgcaaaat
      181 tgttcattca tattgtagtc ctgtcattct tataacgctg atgtgtttat aggtaattga
      241 tttgtgagct cttacacaaa tagcaaggag aaacaatata acatgtaagt aataatgttg
      301 catacttatg atagcatatg ataaatgtag tgcaaccacc cataaatatt gctggaggag
      361 aattaaagtt ataaaataat gttatagctt tatttattca ttttgtttta taggtataaa
      421 tgttattttt tatgtgattt ataaaggttt attgcatgtt tagtccaacg gcaatggtag
      481 gtagggcgtt agctcaggcg gttacacaaa ctcttagacc agcagtaacc aaggctgcga
      541 cgcaagcggg catgggggcc aatgggatga ggttcgcagc tatgcagtcg aaccttatga
      601 ttaatcatgg caaactgact aaccagctac tacaggccgt agccaaacaa acgggtagta
      661 gcgataccca gcaatggttc aagcaggagc agattacttt tttatccaga acagtaaaca
      721 aaactgtgga tgactattgc atgagtaata attcggtaat taataaggag actaaatttc
      781 gcatttttaa agcggttgaa agcgcgatac agcagcctct cgatatgaac tgtgcacaat
      841 catcaattgg tcactttttg caatccaata aatattttaa tcagaaagtt gatgagcagt
      901 gtggtaaagg cgtggatccc attactcgtt ttaacactca gactaagatg atagaactgg
      961 tctcacgcga aatctttgaa cagaatttta acaccgcgaa agtcagtgat atcaaggctc
     1021 taactcagag ggcgattgca gaaaatgtgc aggatactcg gctgtagggg agggacttac
     1081 tgctctgtta tttgtttgtt cttg
//
LOCUS       DAZUFB010000362         1101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZUFB010000362 DAZUFB010000000
VERSION     DAZUFB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.373"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..291
                     /locus_tag="ACHY3M_005484"
     CDS             <1..291
                     /locus_tag="ACHY3M_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4320146.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            294..875
                     /locus_tag="ACHY3M_005485"
     CDS             294..875
                     /locus_tag="ACHY3M_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFS4320147.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            875..>1101
                     /locus_tag="ACHY3M_005486"
     CDS             875..>1101
                     /locus_tag="ACHY3M_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320148.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEA"
BASE COUNT          251 a          297 c          340 g          213 t
ORIGIN      
        1 atgagtgatt tcattactga agatcagcgt ctggtcattc tgcggtcgct ggcagattac
       61 aacggtgaac tgggtgaatc cgtgctccag gactgtctgg atgattacgg ccaccgcgtg
      121 tcccgcgaca ccgttcacac ccacattgcc tggcttgccg agcagggact ggtgcgcaaa
      181 cgcgttctga ttaacggtta tttcatcgcg gaactgaccg gccgggggca ggatgtggcg
      241 gaaggccgcg ccagcgttcc gggtgtgaag aaaccacgcg caagggggta acgatggaca
      301 agccgacgcg tggccgcgtg cgcaaggtgg atttgctccc cgacagcatc cgtaagccac
      361 tgctggaaat gctgcgtgaa aaacgtctga cgcaggtcca gatacgcgag gaaatcaacc
      421 gtctgatccg tgaggcgggc ctgccggaag agcagcagct ttcaccggcg gccatcagcc
      481 gggaagcctc ccgtaatgag ctgattgccc gtaacctgcg tgatttacgc gagcagacaa
      541 aagccatgat ggccgaactg ggggacaagc caaccggtga aaccactgca ttgatcctgg
      601 agatgtcccg cgccctgatg taccgccgtc tgcgtgccgc gaccgaatcc ctgaacagtg
      661 acagcgacgt ggatatgcgt cttattaagg acatcctcct gtcagcacag cgtgcggaaa
      721 gtgccgccga gcgcagcatt aagcgtgaaa aagaaatcag ggctgcattt gctgaagaga
      781 tggcaaacgc cgtcaccgac gagctgcgcg gcgtggacgg gatgagtgaa cagcttgagt
      841 cccgtattaa ggggattctg ttgggtaaag tctgatgaat aacgccgaag aactctcccc
      901 cttactgacg aataccgtct ccacccgcaa aattgatctg gccggtgaaa aagccctgct
      961 gggcgtggat gtgccggaca gccttgattt gcccggtgat atgccggtat ttctggatta
     1021 tcaggcccgc tggtttgagg atgaaagcga ggtctgcatc gcggaaaaat cccgccgcac
     1081 cgggcttacc tgggcggaag c
//
LOCUS       DAZUFB010000363         1090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZUFB010000363 DAZUFB010000000
VERSION     DAZUFB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.374"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..369)
                     /locus_tag="ACHY3M_005487"
     CDS             complement(<1..369)
                     /locus_tag="ACHY3M_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFS4320149.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNG"
     gene            550..702
                     /locus_tag="ACHY3M_005488"
                     /pseudo
     CDS             550..702
                     /locus_tag="ACHY3M_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(727..1089)
                     /locus_tag="ACHY3M_005489"
     CDS             complement(727..1089)
                     /locus_tag="ACHY3M_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320150.1"
                     /translation="MFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALSFILVINN
                     TYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILF
                     TTIMLSAIPSAKMSLSAH"
BASE COUNT          353 a          188 c          206 g          343 t
ORIGIN      
        1 gccatttcca tatctaaaat gttcaatcag atcttcgata agtgttttat attgtccaaa
       61 aaaggaaagc atttttgcta gttctttcat ttctgcaaat aaaatatcag tgcattcacg
      121 ttgtgatatt tttgttccgg aacttgaggc actaaaagca acattaatag aacctgggcc
      181 agggaaatgg tatttaataa gtctgtaagg atcaactttt gcagagatat cgcttaggcc
      241 gagtgagaga aagtcttgtt ctaccatatc accatattgc atatcatcag cagaaaaatc
      301 atcgaataaa ctaatagttt cgaaaagtgt acagggaata ttaattggcg gaagtaatat
      361 gttattcatt tgcatttgcc ttggttatat ttaagctcaa tatcaattcg agaacaaatg
      421 ctagggtaaa aaaatattta aaaaagaagt aaacttcaca ttttttcatt acatctctga
      481 agagaacctc gttaatgatt ggtcttgctc attgttaata acaaaatgtt gaatttaccg
      541 attaggaaac tttttcctac gcggattgcc tccagctatg gcatgaactt tgagtttatg
      601 ccagaactga agtggggctt cggctaccct ggcgcgatta tctttatgat cctcgcgggc
      661 ctgacaccgt atctgtactt taagcgtaag aactggctgt aactatacga tgtcgctgtg
      721 agtaagttag tgtgctgaca gcgacatttt agccgaggga atcgcagata acataattgt
      781 tgtgaataat atccatgcca cgttgagttg gacgcttaat gactcacatc tcaccccttc
      841 aattgctgca gagataatag aaaaaagtgt caagcccaat gaaatcaata caaagtagat
      901 tgccttacat ccagtatctc ttattctttt tgctccaata ttgatattta cataggtatt
      961 attaataacc agaataaaac tcaaagccag gttgatatca ttcaaagtta ttaatattga
     1021 ccatgatgtt aatgtgtaga ttatgaactg aaagcgtgat aaacgctcaa gcctggatgg
     1081 gtagaatata
//
LOCUS       DAZUFB010000364         1082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZUFB010000364 DAZUFB010000000
VERSION     DAZUFB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.375"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..843
                     /locus_tag="ACHY3M_005490"
     CDS             <1..843
                     /locus_tag="ACHY3M_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS4320151.1"
                     /translation="AIPRVGQEVIVDFLNGDPDQPIIMGRTYHHENRTPGSLPGTKTQ
                     MTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNN
                     QMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMN
                     TSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELC
                     CGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPD
                     MREY"
BASE COUNT          296 a          249 c          321 g          216 t
ORIGIN      
        1 gcgataccgc gtgtgggtca ggaggtgatt gtggacttcc tcaacggcga cccggaccag
       61 ccgattatta tggggcgtac ctaccaccat gaaaaccgca cacccggcag cctgccgggg
      121 acgaagacgc agatgacgat ccgctcgaaa acctataagg gcagcgggtt taatgaactg
      181 aagtttgacg atgcgacagg gaaagaacag gtctacatcc acgcgcagaa gaacatgaac
      241 accgaggtgc tgaataaccg caccacggat gtgataaaca accatgctga gaccattggc
      301 aacaatcaga tgattgcggt taccaacaat cagatacaga cggtgggcgt taaccagata
      361 gagacggtgg gcagtaacca gatcattaac gtgggttctg ttcaggtgga aacgattgga
      421 cttgttcgtg cgctgaccgt gggcgtggcg taccagacga cggtaggtgg cattatgaac
      481 acttcggtgg ctctgatgca gtcctcgcag atcggtttgc ataaatcgtt gagggtcggg
      541 ctgggttatg acgtcaaagt cggaaataac gttaccttca ccgttggtaa aacgaaaaag
      601 gatgataccg ggcagaccgc gatttactcc gccggtgagc atctggagct ctgctgtggt
      661 aaggcaaggc tggtgctgac gaaggacgga caaatttttc tcaacggcac aaaaattcat
      721 ttgcagggta aggagcaggt taatggtgac tcactgttga ttaactggaa ctgtgcagcc
      781 tcgaaatccc caccgaagac ccctgatgaa aagcaggata cgccggatat gagagagtac
      841 tgatcccgta cctgttcgga attcagtaac aaaccagcat taaaaataaa cagaagaagg
      901 aatttgaatc atgagcggaa aaccagcggc gcgtcaggga gatatgactc agtatggcgg
      961 tcccattgtc cagggttcgg caggtgtaag aattggcgcg ccaactggcg tggcgtgctc
     1021 ggtgtgtccg ggcgggatga cctcgggcaa cccggtgaac ccgctgctcg gtgcaaaagt
     1081 ac
//
LOCUS       DAZUFB010000365         1068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZUFB010000365 DAZUFB010000000
VERSION     DAZUFB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.376"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..215
                     /locus_tag="ACHY3M_005491"
     CDS             <1..215
                     /locus_tag="ACHY3M_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4320152.1"
                     /translation="REHVQSLTAQLRLGPADILESDENGIIPEQDRVITQVVILDTDK
                     KLIQCVVRPLQILRADGTWENIGGMK"
     gene            321..>1068
                     /gene="nleH"
                     /locus_tag="ACHY3M_005492"
     CDS             321..>1068
                     /gene="nleH"
                     /locus_tag="ACHY3M_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFS4320153.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDR"
BASE COUNT          312 a          184 c          281 g          291 t
ORIGIN      
        1 gccgtgaaca cgtgcagtcg cttacagcac aactgcgact ggggccggca gacatcctgg
       61 agtcagatga gaatggcatt attccggagc aggacagggt aatcacacag gtggtgatac
      121 tggatacaga taaaaagctg atacagtgtg tggtaagacc gctgcaaatc ctgcgtgctg
      181 acgggacgtg ggaaaatatt ggcgggatga agtaacccga cagcttcaca aaaccggagt
      241 ccggctccgg tttttgttgt catgtcatgg tgatgtttgt taagaaagta aagattgatt
      301 cattttgaag gttgaaatgt atgctatcac catcttctgt aaatttgggg tgttcatgga
      361 attctttaac cagaaacctg acttctcctg acagtcgtat tttatcctct gtaagggatg
      421 cagctgctag ctcggataat ggggcgcaag taaaggtggg caacagaaca tatcgtgttg
      481 ttgtcactga taataagttt tgcgttacaa gagaaagtca tagtggttgc tttactaatc
      541 tgttgcacag gctgggatgg cctaagggag agattagcag gaaaattgag gtgatgctga
      601 attcatcgcc agtgaatagg gctatggaaa gaggtgctgt tcattcgaat agacctgatt
      661 tacctcctgt tgattatgca ccgccagagt taccgagtgt ggactataac agcttgcctg
      721 tgcctggtaa tgttattggc aaagggggta acgctgtagt atatgaagat gctgaggatg
      781 caacaaaagt cctgaagatg tttactacat ctcaaagcaa tgaagaggta acaaacgaag
      841 ttcgttgctt caaccaatat tatggtgccg ggagtgcaga aaaaatatat ggcgataatg
      901 gcgatattat tggtattaga atggataaaa taaatggaga atcgctttta aatatttcgt
      961 ccttgccggc acaggctgag catgccattt atgatatgtt tgacaggctt gagcaaaaag
     1021 gaattctttt tatcgataca acagagacaa atgtcttata tgatcgca
//
LOCUS       DAZUFB010000366         1065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZUFB010000366 DAZUFB010000000
VERSION     DAZUFB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.377"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..165
                     /locus_tag="ACHY3M_005493"
     CDS             <1..165
                     /locus_tag="ACHY3M_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320154.1"
                     /translation="GRNPDEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTEQQR
                     QQATDSQHEE"
     gene            155..>1065
                     /locus_tag="ACHY3M_005494"
     CDS             155..>1065
                     /locus_tag="ACHY3M_005494"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4320155.1"
                     /translation="MKNKSWFRMQAGGQGEADIYIYDEIGFWGVTAKQFVSDMNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNHGAAITVYVDGVAASMASLIAMAGDTVIMPE
                     NAFMMIHKPWGISGGDAEKMRTYAERLDKLESVMVPVYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNMITQPYNSAPRDTTVT
                     IPAPAVTEPSPVTAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPA"
BASE COUNT          279 a          263 c          304 g          219 t
ORIGIN      
        1 gggcgcaatc cggatgaggt caaacgtcgc cgcaaggctg aaattgatga aaacagcaga
       61 ctggggctgg tctttgatac tgaccccgtc aacgacaaag gaggcaacag tgccggaact
      121 gaacaacagc gtcagcaggc caccgacagc cagcatgaag aataaatcct ggttcaggat
      181 gcaggcgggt ggtcaggggg aggcggatat ttatatttat gacgagattg gtttctgggg
      241 agttaccgcg aagcagtttg tcagcgatat gaatgccctg ggtgatatca cccacattaa
      301 tctccacatc aactcaccgg gtggcgatgt ctttgaaggc atcgccattt ttaatgccct
      361 gaaaaatcac ggtgcggcca ttaccgtgta tgtggatggc gttgccgcct cgatggcatc
      421 cctgattgcg atggccggtg acacggtcat tatgccggaa aatgccttca tgatgatcca
      481 taaaccctgg gggatcagcg gtggtgatgc ggagaaaatg cgcacttatg ccgaacgtct
      541 ggacaaactt gagtcggtta tggtgccggt atatgcgcag aaaaccggaa aaactaccga
      601 tgaaattgcc gccatgctgg cggatgagac ctggatgtcc ggtgccgagt gtctggcaca
      661 cggatttgca gaccaggtga cgccagccgt taaggcaatg gcatgtattc agtcaaaacg
      721 tacagaggaa tttaaaaaga tgccggaatc catccgaaat atgatcacgc agccatacaa
      781 cagtgccccg cgtgatacca cagtgacaat ccctgcaccg gcggtaacag aaccatcacc
      841 ggtaacggca gtgtctgatg aggcgaccat tcgcgcccgc gttatggcag aacagaaagc
      901 ccgcatgtca ggcattaacg atctgtttgc catgttcggc ggtcgctatc agacgcttca
      961 ggcacagtgc gtggctgatc ctgactgttc gctggaaatg gcccgtgaac gtctgctgaa
     1021 tgaaatgggc aaggagtcct cgccgaccaa caaaaataca ccggc
//
LOCUS       DAZUFB010000367         1065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZUFB010000367 DAZUFB010000000
VERSION     DAZUFB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.378"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..165
                     /locus_tag="ACHY3M_005495"
     CDS             <1..165
                     /locus_tag="ACHY3M_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320156.1"
                     /translation="GRNPDEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQY
                     QRDTESQHEE"
     gene            110..>1065
                     /locus_tag="ACHY3M_005496"
     CDS             110..>1065
                     /locus_tag="ACHY3M_005496"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4320157.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPA"
BASE COUNT          275 a          260 c          303 g          227 t
ORIGIN      
        1 gggcgcaatc cggatgaggt caaacgtcgc cgcaaggctg aaattgatga aaacagcaga
       61 ctggggctgg tctttgatac tgaccccgtc aacgacaaag gaggcaacag tgccggaact
      121 gaacgacagt atcagcgcga caccgaaagc cagcatgaag aataaatcct ggttcaggat
      181 gcaagctggg gggccgggtg acgcggatat ttatatttat gacgagattg gtttctgggg
      241 agttaccgcg aagcagtttg tcagcgaact gaatgcactg ggtgatatca cccacattaa
      301 tctccatatc aattcaccgg gtggcgatgt ctttgaaggc atcgccattt ttaatgccct
      361 gaaaaatcag ggggcgacca ttaccgtgta tgtggatggc gttgccgcct cgatggcatc
      421 tgtgattgcg atggccggtg atacggtcat tatgccggaa aatgccttca tgatgatcca
      481 taagccatgg ggattcagtg gcggggatgc tgaggatatg cgcagttatg ccgatttgct
      541 ggataaagtc gaatcggtac tgttgccagc ctatgcgcag aaaaccggaa aaaccaccga
      601 tgaaattgcc gccatgctgg cggatgaaac ctggatgtcc ggtgccgaat gtctggcaca
      661 cggatttgct gaccaggtga cacccgctgt tgaggcaatg gcatgtattc agtcaaaacg
      721 tacagaggaa tttaaaaaga tgccggaatc catccgaaac atgattactc cgccacgcaa
      781 cagtgccccg cgtgatacca cagtgacaat ccctgcaccg gcggtaacag aaccatcacc
      841 ggtaccggca gtgtctgatg aggcgaccat tcgcgcccgc gttatggctg agcagaaagc
      901 ccgcatgtca ggcattaacg atctgtttgc catgttcggc ggtcgctatc agacgcttca
      961 ggcacagtgc gtggctgatc ctgactgttc gctggaaatg gcccgtgaac gtctgctgaa
     1021 tgaaatgggc aaggagtcct cgccgaccaa caaaaataca ccggc
//
LOCUS       DAZUFB010000368         1064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZUFB010000368 DAZUFB010000000
VERSION     DAZUFB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.379"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1064)
                     /locus_tag="ACHY3M_005497"
     CDS             complement(<1..>1064)
                     /locus_tag="ACHY3M_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4320158.1"
                     /translation="SRMGSEYYRTLQNGGRQAAAAARDQRRALAELNSQLTEIRGSAV
                     GMAGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFSSSQKVLMDISQRTGTAFS
                     DNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASTAEAGSVITQFSQALAQG
                     VLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLEVLR
                     DEYAAMPETVSDGITKVENAFMAWVGGANEASGVTKTLSGVLNGVAGQIDNVATAVGA
                     LVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFRGTQIATARARAAVYRAQQ
                     AVAAARGTEMQIAAEARLAAT"
BASE COUNT          220 a          368 c          261 g          215 t
ORIGIN      
        1 gtggccgcca gacgggcttc tgcagcaatc tgcatctccg tcccgcgggc tgccgccacg
       61 gcctgctgag cacggtacac ggctgctctt gcccgcgccg tggcaatctg cgttcccctg
      121 aactgtgctt ccgccagtgc aacttcatta cgtgcagccg tcacaagtcc tgccgtggca
      181 gacatcgctc cggaggccat attgccaaag taccgggcaa ccccgacggc aaccagtgcc
      241 cccacggctg ttgccacatt atcaatctgt ccggcaacac cgttcagcac gccggagagc
      301 gttttcgtca ccccgctggc ctcattcgca ccacccaccc aggccataaa ggcgttttcc
      361 acctttgtga tcccgtcaga gaccgtttcc ggcatggccg cgtattcatc acgcaatacc
      421 tccagctggc tgattaacgc aggaacgact ttatccgccg tcagttgacc atcgtccgcc
      481 atcgccttca gatctttacg ggccacgccc atacccgcag ccagtgcacg tacgatccgg
      541 tcaccacttt cattgaccga attaaattcc tcaccgcgca acacaccctg tgccagcgcc
      601 tggctgaact gggtgatcac cgaacccgcc tcagccgtac tggcaccgga gattttcagc
      661 cccgtggaaa tggcctccgt caccttcagc acatcatcag cactgtaacc atattcacgc
      721 attgaggctg ccgagcgggc aaacagggcc gcattatccg aaaaagcggt acctgtccgc
      781 tgactgatat ccatcagcac tttctgtgat gacgaaaatt catcagatga ctgcgacgcc
      841 tgtttcagac gggcattcac ggaactccac tcatcagcca gtgaaatcag gtgtccggtg
      901 gcaaaggcac cggcaaatgc gccagccatt ccgacagcag aaccgcgaat ttccgtcaac
      961 tggctgttca gttctgccag ggcacgtcgc tgatcccggg ctgccgcagc ggcctgacgc
     1021 ccgccattct gcagggtccg gtaatattca ctgcccatac ggga
//
LOCUS       DAZUFB010000369         1035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZUFB010000369 DAZUFB010000000
VERSION     DAZUFB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.380"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..795)
                     /locus_tag="ACHY3M_005498"
                     /pseudo
     CDS             complement(1..795)
                     /locus_tag="ACHY3M_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          320 a          200 c          206 g          309 t
ORIGIN      
        1 ttacagaacc tgaagtcact gtggaatcgg taactgagat attgttatcc caagtatgag
       61 attcagtagt gtaagtattg tttacggtat taccctgagt ctgataccag caacgcggga
      121 gttgttagaa atattgatgt tactttcgac atcaagattc actgccagcc caaggccaac
      181 accaccgttt gcaaacaaac cggcgtattc ttcaccttca ccagctgggg tatggaaggt
      241 ttcgttagta tatttggtaa cgtcaccgtc cagatatgaa tcagtgaagt ataaaccttc
      301 ataatcgtca tcaattgtag aattagcaat atttaaagtg aatacatcac catcatgcca
      361 attatcatca attacacgat cagtaccgta ttcagagaac tgatagacat aatgaccatc
      421 actgctttca cccataggca gcattgaagt gattgaacca tgaatcaggg agttgctgat
      481 gttcaactgg tttgttattc catcagtaaa gtcatgattc aggtaatatg tagagatagt
      541 cccgttaaca gtgtcaccat taaaggtggt atcgaaatca ccgtttgtgt tgttatagta
      601 gacaaagcca ctataattca catagttacc atctacagta cccggtgcat ccatattgct
      661 ccagtcaaga gcggtagttg gattgtcgta ataagtccag gtgtcataag tagttgcatt
      721 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
      781 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
      841 caatgcatag aaaacatatt atgtttcgcg ctaatgatta tgctgcgtta cagacgaatg
      901 gttcaattat tctttcccct aagtccgaat gtggacatat ataacctatg gagaaaaaca
      961 cgcatcaaat tgataaaagg gcgatatttc aacatgttgc agataatgtt atttttgata
     1021 catacgcgtt ataaa
//
LOCUS       DAZUFB010000370         1032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZUFB010000370 DAZUFB010000000
VERSION     DAZUFB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.381"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..588
                     /locus_tag="ACHY3M_005499"
     CDS             <1..588
                     /locus_tag="ACHY3M_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFS4320159.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            560..943
                     /gene="yhhH"
                     /locus_tag="ACHY3M_005500"
     CDS             560..943
                     /gene="yhhH"
                     /locus_tag="ACHY3M_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFS4320160.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          372 a          150 c          231 g          279 t
ORIGIN      
        1 tatatcactc aggatccgat tggactgaag gggggatgga acctgtatgg atatcaattg
       61 aatccgatat cagacatcga ccccctgggt ttatctatgt gggaggatgc aaaatcgggg
      121 gcatgtacta atggtctttg cggcacacta tccgctatga taggtccaga taaatttgat
      181 tctatagata gcaccgcata tgacgcctta aataaaataa atagccaatc tatttgcgaa
      241 gataaagagt tcgctggttt aatatgtaag gataatagtg gcagatattt ctcaacagca
      301 cctaaccgag gagaaagaaa aggatcatat ccattcaata gcccttgccc taatggtact
      361 gagaaagtat cagcttatca tactcatggt gcagatagtc atggagaata ttgggacgaa
      421 atattttcag gtaaagatga gaaaatagtt aaaagtaaag ataacaatat caagtcattt
      481 tatttaggta cgcccagtgg taattttaaa gcaatagata accacgggaa ggaaataaca
      541 aacagaaaag gattacctaa tgtctgcaga gttcatggta atatgtaaaa aaatattgtt
      601 taggaactgt gtcattgtat ctttgtttgt ttttacatac aacacatggg cgcagtgtaa
      661 taataatatt aaaataatgc gcaagtatga aagtgaaggt aaatataccg ttagaaattt
      721 ggttaaaaat aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg
      781 acaggatgcc gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt
      841 tgttgaaggt accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg
      901 caaaaatgat gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca
      961 aacatcgtgt gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa
     1021 aagatagcct tt
//
LOCUS       DAZUFB010000371         1028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZUFB010000371 DAZUFB010000000
VERSION     DAZUFB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.382"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..257
                     /locus_tag="ACHY3M_005501"
     CDS             <1..257
                     /locus_tag="ACHY3M_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFS4320161.1"
                     /translation="RVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACE
                     AIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            531..>1028
                     /locus_tag="ACHY3M_005502"
     CDS             531..>1028
                     /locus_tag="ACHY3M_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4320162.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRL"
BASE COUNT          288 a          215 c          277 g          248 t
ORIGIN      
        1 tcagagtgcc actgaccgaa ccccagaaag cggggattgc gtcattctgt ccgtacaaca
       61 ttggccccgg taagtgtttc ccgtcgacgt tttataaacg aattaatgca ggagatcgaa
      121 aaggtgcctg cgaagcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtattc
      181 gctcaaataa ctgttatggt caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct
      241 ggggaatcga cagataagca gaatattttg ctgaaaaatg acgttggcca gcgcgggcgg
      301 ataacacgaa atcctgcgaa ctggcaaaat gtaagtgaat aaagttaggc agattatttc
      361 acgcagaggc accgtaatgg tgcctttgtc atttctgcgc ttcgcacaag cgtaaataaa
      421 ccaaagaacc tttcaggatg agccctggtg gataaccggc agtggtctgg ttaaccctct
      481 ttgggctggt tattcctgtg cgcagggttc atcactaaaa ggaatcaacc atgaaagaga
      541 tgatttctgt cgatcatgaa atatccatga gtagtctgga ttttctgaat aacattatta
      601 atccagcccg ggcagaagcc ggagaagtcc ctcatgaacc gcgtaagttt cttgcaaaaa
      661 ttgaggatga gctagagctt gatggaaccg gaaaaaaatt ccggttaaac aataaccaga
      721 caagaacggc atactatgat ctggattttg accagatgat gctcgttggc atgagggagt
      781 caaaggccgt tcgtcgttct gtgctggcaa gactcaaagc gatgcatggt attcagatcc
      841 cccggacttt acctgaggcg ttgcgatttg cggcaaaact ggctgaacag aaagcagtgc
      901 tggaaaatca actggcaata gcagcgccga aggctgaatt tgttgataac tatgttgaag
      961 catctggtct gatgggattc cgggaagttg ctaagttact cggtatcaaa gaaaccgatt
     1021 tccggctg
//
LOCUS       DAZUFB010000372         1007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZUFB010000372 DAZUFB010000000
VERSION     DAZUFB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.383"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(221..910)
                     /locus_tag="ACHY3M_005503"
     CDS             complement(221..910)
                     /locus_tag="ACHY3M_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4320163.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFATSSWRRTLLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWNEFKTQSGTRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(907..>1007)
                     /locus_tag="ACHY3M_005504"
     CDS             complement(907..>1007)
                     /locus_tag="ACHY3M_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320164.1"
                     /translation="RFNEQFDEINIVRGKPVSGGRLEIKITEVGCA"
BASE COUNT          249 a          281 c          191 g          286 t
ORIGIN      
        1 gcgtttctca aactttcctt tttccggtta acgtgacaca ccaataactc ttgtcgaaaa
       61 agccagcaag ctgaaagacc ggtattcacc gccgccagcg cgtttactgt actgacgcga
      121 ttttcggaca taaaaaaaac gcctggcggc agtttttttc ttactttgcc atcgcgtaca
      181 aaataggcaa aatatcagat ttatacgaaa catacgcgat ttaattgact tttgcaatat
      241 ctcgtcgtga aaaggtcgct ttttgttgcg ctcttatttt cacggagcaa atcaaggatt
      301 ctctatcgag gcgcttaaaa atatcgcaca tctcacgcca gtagttcgca taattatggc
      361 tccagttatc aggcttaact ccacacagtc tggcaagctc ctgtctctgg tagacctcac
      421 acccggtaac ccatcctctg acatcctgtg ccgccagcca gatcaacttc ttcacacgct
      481 ccagcgtttt cactgcaatt tttctggtac cagactgagt tttaaattca ttccacaccc
      541 actgcgttat cgcgatctga tgctcccagc aaatgtttcc gccataacac cacaacaacc
      601 acgccttctg atgttcttcc agttccagaa gggtacgccg ccacgatgat gttgcaaact
      661 caacaggact gaccagggca attgatgagc ctttcgccag tgattgttta cccgggattg
      721 ggggattatc ccgcgtgatc atttttccgg ttacctcatc gcggtaacga atttttttgc
      781 gtctgtaacg ccctgtatcg aacagggcat tttcctgcca ggcttccagt tgaccttttg
      841 tcgccccact gagatccgca gtggcaatca tgagttgctc acgaacaaac tgtaaatact
      901 ggttattcat gcacacccca cctctgtaat ttttatctcc agccgtccac cagatactgg
      961 cttgccacgt acaatattga tttcatcaaa ctgctcattg aaccgcc
//
LOCUS       DAZUFB010000373         1005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZUFB010000373 DAZUFB010000000
VERSION     DAZUFB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.384"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1005
                     /locus_tag="ACHY3M_005505"
     CDS             <1..>1005
                     /locus_tag="ACHY3M_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4320165.1"
                     /translation="RENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAV
                     QHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRF
                     SGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVY
                     DPTVQFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVG
                     KSAFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNV
                     SNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGI
                     "
BASE COUNT          247 a          261 c          299 g          198 t
ORIGIN      
        1 tccgggaaaa cacggacggc acctttgcca tcacggcggt gcagcacgta ccggaaaaag
       61 aagccatcgt ggataacggt gcccgctttg agccgcagtc aggttccctg aacagcgtca
      121 tcccaccggc agtacagcac ctgacggtgg aggtgagtgc agctgacggc cagtatctgg
      181 cgcaggctaa atgggacacg ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga
      241 ccagtggtaa gggaacggat gccagactgg tgaccaccgc catcaccgca gacacggagc
      301 accgtttcag cggcctgccg ctcggggaat acaccctgac ggtgcgggcg ataaacagct
      361 atggccagca gggtgaacct gccaccacca ccttccggat tgccgcaccg gcagcaccgt
      421 cgcggattga gctgacgccg ggctattttc agataaccgc cacgccgcat cttgccgttt
      481 atgacccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg gatatcaggc
      541 aggttgaaac cgcagcccgc tatcttggct cggcgctgta ctggatagct gccagtatca
      601 atatcaaacc gggccatgat tattattttt atatccgcag tgtgaatact gttggcaaat
      661 cggcattcgt ggaggctgtc ggtcgggcga gcgatgatgc ggaaggttac ctggattttt
      721 tcaaaggaga aatcgggaaa acacatctgg cccaggagct gtggacgcag attgataacg
      781 gtcagcttgc gccggacctg gctgaaatca ggacgtccat tacgaatgtc agcaatgaaa
      841 tcacgcagac cgtcaataaa aaactggaaa atcagagtgc ggcaatccag cagatacaga
      901 aagttcaggt tgatacaaat aataacctga acagcatgtg ggccgtgaaa ctgcagcaga
      961 tgcaggacgg acgcctttat attgcgggta tcggtgccgg tattg
//
LOCUS       DAZUFB010000374          997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZUFB010000374 DAZUFB010000000
VERSION     DAZUFB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.385"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..576
                     /locus_tag="ACHY3M_005506"
     CDS             13..576
                     /locus_tag="ACHY3M_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4320166.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEESG"
     gene            573..>997
                     /locus_tag="ACHY3M_005507"
     CDS             573..>997
                     /locus_tag="ACHY3M_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4320167.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAAWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDD"
BASE COUNT          241 a          251 c          314 g          191 t
ORIGIN      
        1 cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg
       61 aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg cgaagacagc cagggttaaa
      121 ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta tagcccgtaa acagtgcgag
      181 acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagcc ggaggataaa
      241 ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaacaaatcc ggagatgcca
      301 ccgcccgtgg tgatatctcc tggtattgag tatatggagg acggtcttcc cgatccggtg
      361 aaagcgatgg ggcgtcttct ggtggagaac attaataccg accccaggct ggcgctggat
      421 gcggcttata agctggcgca attcacgcac cacaaaaaag gggatgccgg taaaaaatcg
      481 gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg cggtgccacc accaccccgc
      541 ctggtggtga ataatgataa tgaggaaagc ggatgatacc tgtgtggagc acggcatgtc
      601 cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggctccg atttatccgg
      661 accaggctgc acatgcactg gcgattttta aacaactgcg gattgtggat gcaccgggta
      721 gcccgacatt cggggagtcc tgtgcagcgt gggtgtttga cctggtggcg gccctgtttg
      781 gctcctacga tgcgcagacc ggtgtacgcc atatcaagga agtttttatc cttatcccca
      841 agaaaaactc gaagtccacg ctggccgcgg ggatcatgat gacggcgctg ttactgaact
      901 ggcggcaggc ggcgggctac accattctgg ccccgaccgt ggaggtggcg gctaacgcct
      961 tcaaccctgc cagggatatg gtacgacggg acgatga
//
LOCUS       DAZUFB010000375          993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZUFB010000375 DAZUFB010000000
VERSION     DAZUFB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.386"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..122
                     /locus_tag="ACHY3M_005508"
     CDS             48..122
                     /locus_tag="ACHY3M_005508"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320168.1"
                     /translation="MKSSHFTAQVEGAYRLIRNPSVSP"
     gene            297..>993
                     /locus_tag="ACHY3M_005509"
     CDS             297..>993
                     /locus_tag="ACHY3M_005509"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4320169.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVK"
BASE COUNT          249 a          263 c          291 g          190 t
ORIGIN      
        1 ttgtgtcact gacctcttct ctcgctcagc atgcaggtct ttctattgtg aaatcatccc
       61 acttcactgc tcaggttgaa ggtgcgtacc gcctgatacg taacccgtcg gtgtccccgt
      121 aagccattgc cgaagccgga tttactgcca ctgcaagggc atgtgaggca catccgctgc
      181 tcctcgcgct ggaagatacc accaccatta acttcggtca cagtacggca tttgatgacc
      241 tggggaatac caccactaac ccgaagacgc acgggctgct ggctcactct gtcctgatgt
      301 acgcccccga cagcgccctg ccagtcgatc ttatcgaaca gcagtgctgg agtcgtgcca
      361 cggacaccta cggtgttaag caccagcgaa aagaacgtcc ctgtgaagaa aaagaaagtt
      421 accgctggca gcaggcttcg gagcgcatgg ccgaacggct gggggagata cagaaacagg
      481 taatcactgt ctgtgaccgg gaagcggata tctggcatta cctgcattat aaagtcagcc
      541 gcgggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg ccaggcccgg
      601 atgctcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg ccaggcgctc
      661 tcgctcacgt atgtctgttg cggggagcag gagaggacgg agcctgctgg tatccgctga
      721 catcagaaaa agtggcgagt gccgccgatg cgcgacgtat cgtcagccat tacgagcaac
      781 gctggctgat agaggaatac cacaaagcgt ggaaaagtgg tggtacatgt gtggaatcgc
      841 tgcgaatgca gacccgggat aaccttgagc gcatggtggt tatcgcggtg cgggtgctgg
      901 gcctgcggca gggaggtatc agtgaagaaa cacaaaatga cagctgtgag aaaatactga
      961 cgccgacaga gtggaaacta ctgtgggtta agc
//
LOCUS       DAZUFB010000376          974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZUFB010000376 DAZUFB010000000
VERSION     DAZUFB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.387"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..633
                     /locus_tag="ACHY3M_005510"
     CDS             <1..633
                     /locus_tag="ACHY3M_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320170.1"
                     /translation="SIGSTPSVEVQSIHAGFDWDSGKVLIFPSQPLTTLTPEQITDIT
                     DSVRKGQSWHAYQEYKKHQEQLEKLSIELDAAKQRIAELEGNRTALAVENELARKAVQ
                     AFCDVVGDNTEVIAEVVGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVDAAIEAAK
                     NLVAQEYEYKDFKAAQSDCCMHPGSDLVGKVEMTEWLVDFAAQLRKGGNQ"
     gene            615..974
                     /locus_tag="ACHY3M_005511"
                     /pseudo
     CDS             615..974
                     /locus_tag="ACHY3M_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT          269 a          197 c          285 g          223 t
ORIGIN      
        1 agcattggtt caacaccttc agttgaggtt cagtcaattc acgctggttt tgattgggat
       61 tccgggaaag tgcttatttt cccatcacag ccactgacca cgctaacacc agaacagatt
      121 actgatatca ctgatagtgt gcgcaaaggt cagtcttggc acgcatatca ggaatacaag
      181 aagcatcaag agcagttgga aaaattgtcg attgaactgg atgccgcaaa acagcgcatt
      241 gcagagctgg agggtaatcg cacggcgctg gcagtggaga atgagctggc tcgtaaggca
      301 gttcaggcat tctgcgatgt tgttggcgac aacaccgagg ttatcgctga ggtggttggg
      361 cgagatggcg ttctggttat tttggaggcc atgaaggcaa caggaaatat gccagccacc
      421 gatgctttcc tggctgaagt gcgggcgcag ggcgtggatg ctgctataga agctgcaaaa
      481 aatctggtgg cccaagaata tgagtataag gatttcaaag cggcgcagag tgattgctgt
      541 atgcaccctg gttcagacct ggtagggaag gttgaaatga ctgagtggtt agttgacttt
      601 gctgcccagc ttcgcaaagg aggcaaccag tgagcgaaat taattaccag gcactgcgtg
      661 aggcggcgga acgtgcaatt ccagcaatgg aacgcctgtt aatgttgcca gctgatgatg
      721 atttgttaag tgaacaggaa cttaaagatt acggtgtgga tattgatgcg ctcaacgcct
      781 tcaaatttct ggccggacca gaaaccgtgc tggcactact ggatgaacgg gaaaggaacc
      841 agcaatacat caaatcccgc gaccaggaga acgaggatat tgcgcttacg gttgggaagc
      901 tgagagttga gcttgaggag acaaaatcaa aactcaacga gcagcgtgag tattacgaag
      961 gtgttatctc ggat
//
LOCUS       DAZUFB010000377          971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZUFB010000377 DAZUFB010000000
VERSION     DAZUFB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.388"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            327..923
                     /gene="fimE"
                     /locus_tag="ACHY3M_005512"
     CDS             327..923
                     /gene="fimE"
                     /locus_tag="ACHY3M_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFS4320171.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          281 a          176 c          205 g          309 t
ORIGIN      
        1 atggtttatg ttcttcaaaa taaataaaca aaatcattca taaatttaca catcacttaa
       61 actctcctgt ttccgcactt ttttctttat tttttaagca actggaagtt aatccactgc
      121 aatctattgt tatattgaat caaatcaatg aaaatagatg ttgtcacatc agtgatattt
      181 tatttttgta tgatatttaa tgtaattgac tgatagccac atcactccgt gtgtggttat
      241 ctttttatct attgggctaa ttttgaccga ttgaggtttc ctataggtat tcattcaaat
      301 atatctcagt taggagtact actattgtga gtaaacgtcg ttatcttacc ggtaaagaag
      361 ttcaggccat gatgcaggcg gtttgttacg gggcaacggg agccagagat tattgtctta
      421 ttctgttggc atatcggcat gggatgcgta ttagtgaact gcttgatctg cattatcagg
      481 accttgacct taatgaaggt agaataaata ttcgccgact gaagaacgga ttttctaccg
      541 ttcacccgtt acgttttgat gagcgtgaag ccgtggaacg atggacccag gaacgtgcta
      601 actggaaagg cgctgaccgg actgacgcta tatttatttc tcgccgcggg agtcggcttt
      661 ctcgccagca ggcctatcgc attattcgcg atgccggtat tgaagctgga accgtaacgc
      721 agactcatcc tcatatgtta aggcatgctt gcggttatga actggcggag cgtggtgcag
      781 atactcgttt aattcaggat tatctcgggc atcgaaatat tcgccatact gtgcgttata
      841 ccgccagtaa tgctgctcgt tttgccggat tatgggaaag aaataatctc ataaacgaaa
      901 aattaaaaag agaagaggtt tgatttaact tattgataat aaagttaaaa aacaaataaa
      961 tacaagacaa t
//
LOCUS       DAZUFB010000378          957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZUFB010000378 DAZUFB010000000
VERSION     DAZUFB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.389"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>957
                     /locus_tag="ACHY3M_005513"
     CDS             <1..>957
                     /locus_tag="ACHY3M_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFS4320172.1"
                     /translation="EHLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASAL
                     IGHLRRQSVILPALNAVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNG
                     KTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQM
                     TPADLAKFEPQRRYATLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQAS
                     GKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFD
                     FLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVLDAIEVLR"
BASE COUNT          188 a          329 c          284 g          156 t
ORIGIN      
        1 gcgagcacct gagcgagctg caaaccgtgt tcggtttccg gcccttcacc atgagccatt
       61 accggcaggc cgtccagatg ctgaccgagc tggcgatgca aaccgacaaa ggcatcgtgc
      121 tggccagcgc cttgatcggg cacctgcggc ggcagtcggt cattctgccc gccctcaacg
      181 ccgtcgagcg ggcgagtgcc gaggcgatca cccgtgctaa ccggcgcatc tacgacgcct
      241 tggccgaacc actggcggac gcgcatcgcc gccgcctcga cgatctgctc aagcgccggg
      301 acaacggcaa gacgacctgg ttggcttggt tgcgccagtc tccggccaag ccaaattcgc
      361 ggcatatgct ggaacacatc gaacgcctca aggcatggca ggcactcgat ctgcctaccg
      421 gcatcgagcg gctggttcac cagaaccgcc tgctcaagat tgcccgcgag ggcggccaga
      481 tgacacccgc cgacctggcc aaattcgagc cgcaacggcg ctacgccact ctcgtggcgc
      541 tggccaccga gggcatggcc accgtcaccg acgaaatcat cgacctgcac gaccgcatcc
      601 tgggtaagct gtttaacgct gccaagaata agcatcagca gcagttccag gcgtcaggca
      661 aggccatcaa cgccaaggta cgtctgtacg ggcgcatcgg tcaggcgctg atcgacgcca
      721 agcaatcagg ccgcgatgcg tttgccgcca tcgaggccgt catgtcctgg gattcctttg
      781 ccgagagcgt caccgaggcg cagaagctcg cgcaacccga tgacttcgat ttcctgcatc
      841 gcatcggcga gagctacgcc accctgcgcc gctatgcacc ggaattcctt gccgtgctca
      901 agctgcgggc cgcgcccgcc gccaaaaacg tgcttgatgc cattgaggtg ctgcgcg
//
LOCUS       DAZUFB010000379          954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZUFB010000379 DAZUFB010000000
VERSION     DAZUFB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.390"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..423
                     /locus_tag="ACHY3M_005514"
     CDS             <1..423
                     /locus_tag="ACHY3M_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFS4320173.1"
                     /translation="YAPELRDEHGRKVLISYDIHDAERITVRRLDGSVICEAVWDGNK
                     REAFPVSAEYYKQQQRLKGMRKRAEEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNT
                     VAVQQPAVDDEPDEEYERNFQRGLQLLEAKLKENDPLA"
     gene            494..>954
                     /locus_tag="ACHY3M_005515"
     CDS             494..>954
                     /locus_tag="ACHY3M_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4320174.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKG"
BASE COUNT          256 a          229 c          277 g          192 t
ORIGIN      
        1 tacgcgcccg agctgcgtga tgagcatggc cgcaaggtgc ttatcagcta tgacattcat
       61 gatgccgaac gaattaccgt acgtcgcctg gatggcagcg tgatttgcga ggcggtatgg
      121 gacggtaata aacgcgaagc cttcccggtt agcgcggaat actacaaaca gcagcaacgc
      181 cttaaaggca tgcgtaaacg cgcagaggaa aaaatccgtg atgccgagga tgaggttgtc
      241 aacgtgctgg agcacaagcc gcaggagccc tggctggaaa acatctatcg cccggtaggt
      301 aatacggtgg ccgttcagca acctgccgtt gatgatgagc ctgatgaaga atacgagcgc
      361 aatttccagc ggggattgca gttgctcgaa gcgaaattaa aagaaaatga cccgctggcc
      421 tgaaataaaa aataacccga gcggcgactc aggttatttg attaaacaag gtatcaaatg
      481 agaggttaat aatatgactg atattaacga tgtaatcaag accattgatg aacttattga
      541 tggcggcgta ctgacgcagt atgccatcgc cagagaggcg ggaatttccg acggcacatt
      601 atcggctttc cgcaagggga aatataaagg cgataacgcc gctgtggctg cttccctgcg
      661 ttcctggtat gagaactgga ataaacaaag cgcactgccg gaaccgccgc agtttgtgga
      721 aacgcagaca gttcaggagc tgcgcgcact gtttcaggcg gttcgcctga tgggctgtat
      781 taacgtcatt gtgggcgtgc cgggggtagg taaaacggcc accgcccgta attactgcca
      841 ggagcaacca aacacctgga tgatcaccct gtcacccgcg cactccagcg tcacggagtg
      901 tctgctggag ctggccgatg cgctggggat tgattacacc cgcgcgaaca aagg
//
LOCUS       DAZUFB010000380          946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZUFB010000380 DAZUFB010000000
VERSION     DAZUFB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.391"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>946
                     /locus_tag="ACHY3M_005516"
     CDS             <1..>946
                     /locus_tag="ACHY3M_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320175.1"
                     /translation="KGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEA
                     NKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLTEIQ
                     KQELLRNATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMREL
                     QQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAESDVQRA
                     DWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVL
                     QEISKVLMNAAIVNGIRTAANSMSGAGGFLGSIGDWLGG"
BASE COUNT          264 a          206 c          314 g          162 t
ORIGIN      
        1 aaaggggaag aaaaagagcg tgtccgactg gggtatgcag cggatgacct cggttttgtg
       61 ggtgatccgt atcaggaggc gagacaacgt tatatcagta atgccctgga agcctggcgc
      121 aataacgagg cgaataaacc caaatcccgg ggtggaaaat cagagacgga aaaagcggaa
      181 gacagttttt cccggctgct gaagcagcag aaagagcaac tggcactggc cggtcagaat
      241 acggagctgg cgaagctgaa gtaccagaca gcgctgggtg agctgaaaac cctgacggag
      301 atacagaagc aggaactgct gcgcaatgcg accctgattg accagcaaaa aatccgggaa
      361 cagttgcgat cccgggaaga gacactgaag aatgagaatg cggctgcgcg tgcgtcgaat
      421 gatgctgaac tgctggggta cgggcagggg gaacgagcca gagaacgcat gcgggagttg
      481 cagcagatcc gcgacagctt ccgccagaag gatgcggacc ttcagtctca gtatcagacc
      541 ggggatatca gtgaggattt ttacagacag gcacgggcac agaacgcgca gtatctgagc
      601 gaacgcctta aggaccaggc agccttttat gccgaatcgg atgtgcagcg tgcggactgg
      661 cagaaagggc tgcaggaggg attcagtaac tgggtggata atgcgtccga ttacgcctca
      721 caggcagcac agcttgcgac ggagggtatc tcagggatgg tgaataacat cacggagatg
      781 ctgaacggaa ataaagtgga atggcgcagc tgggcctcat cagtgctaca ggaaatatca
      841 aaagttctta tgaatgccgc gattgtcaac gggatcagga cggcggcaaa cagtatgtcc
      901 ggtgcaggag gatttctcgg cagcattggt gactggctgg gcgggg
//
LOCUS       DAZUFB010000381          930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZUFB010000381 DAZUFB010000000
VERSION     DAZUFB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.392"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(478..>930)
                     /locus_tag="ACHY3M_005517"
     CDS             complement(478..>930)
                     /locus_tag="ACHY3M_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFS4320176.1"
                     /translation="KSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKD
                     MPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSV
                     YYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          339 a          205 c          114 g          272 t
ORIGIN      
        1 tctgaatatc aattcataaa atatgattct ccacgatttg cccagaaata acgaacataa
       61 ttttcgttgt aattaaaatc atatttcatc tatagtgacc attcacagca aaacaaaatc
      121 aattatcaca ttcaaaaaaa tactgacaac aaagatactc ttcagacaaa gttgtcatat
      181 gtaaattttc ccttaaaacg ttgctcaaga aaaatataaa aatcatatgc cctaatcaac
      241 atcctcaagc actacgatat atgcagtatc ctattcatat attaccacca tatcagacaa
      301 atcgaatcac aacgagttct atcgtactct ttcatcttcg acactgtaaa ttttaagaac
      361 attcggctca tgccactaaa ccaagacata ataacaacgg agcaataaat gttcccactg
      421 aacatgtgca aacctaaaaa gacagtgtta caataatatt catccatggt ctctaaatca
      481 acgccacgca acaggataat acaatgaaaa tatttcagga aaagattctt ttgcatgctc
      541 cgttagcata tcagtcagca cagcggtaca agtaaaaaca ttatttcttc cttgtagcct
      601 gtctaaaata tcattgtaat aataaactga gccaaatacc tgaggatctg ttttataagc
      661 ttgttcaatc aatccatttg catatctccg tagaatctca ggagaatcat actctgtacc
      721 aaacatatca ctcgatgagt atttacagaa aaccccccct aaacaaaata atatttcagc
      781 ctgttttctt agtggcatat ctttcaatac atggctcccc ataatcatat ctacatgctg
      841 agcgttcagt tcccaaccat caagaaaggg attccagaca caggcaagtt tcctttgctg
      901 atcatcagca atcattttat aatctgattt
//
LOCUS       DAZUFB010000382          929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZUFB010000382 DAZUFB010000000
VERSION     DAZUFB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.393"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>929
                     /locus_tag="ACHY3M_005518"
     CDS             <1..>929
                     /locus_tag="ACHY3M_005518"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS4320177.1"
                     /translation="VSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGY
                     SRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDIL
                     YRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINN
                     NSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINTYDNTIRYT
                     DYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLIT
                     WMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFS"
BASE COUNT          314 a          152 c          176 g          287 t
ORIGIN      
        1 tgtttctcct ggcgagtttc gtcagactgg aacggatgca tcccgcacaa taaatgaaaa
       61 acaaaagccg gtaattatgt ttttagttgt cggggagaca gcaagaagtc aaaactatgc
      121 tctgaatgga tattctcgtg gtaccaatga ttttacaaag aaatataatg aacttatatc
      181 ctttcataat gtgcagtcgt gtggcacatc cacagccata tctgttccat gcatgttttc
      241 agatatgaag cgaaaagagt tcaattcccg caaagcggta aactcggaaa atgtactgga
      301 tattctgtac agaacggggg taaatttact ctggattgaa aatgacgggg gatgtaaagg
      361 tgtatgtaag cgaatcccta caattaatat tgagccatca aattcagata atacattatg
      421 caagaaaaac tcatgttatg atgaggttat gctaaaaaat attgatgagt atataaataa
      481 taactcagag gataaactta tcgtatttca tttgatgggt agtcatggtc ctacctacta
      541 tctccgttat cctgagtcac ataaatattt taaaccaaca tgtgatcgta gtgatatcga
      601 aaattgtacg catgagcaat tgattaatac ctatgataat acaattcgct atactgatta
      661 tataatatca aaattaattg acaagcttat cgaatataaa gatgagtatg atactgtttt
      721 attatatgtc tctgaccatg gagaatctct gggagaaaat ggattatact tacatggaac
      781 accatataat gttgctccag ctgagcaaac gcatgtgcct cttattacct ggatgtctcc
      841 ggggttcgtt tcctctaaaa aaattgattt gaattgtttg tcagaacatg ctctaaacag
      901 aacagtaagt catgataata tattttcat
//
LOCUS       DAZUFB010000383          926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZUFB010000383 DAZUFB010000000
VERSION     DAZUFB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.394"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>926
                     /locus_tag="ACHY3M_005519"
     CDS             <1..>926
                     /locus_tag="ACHY3M_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4320178.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMA
                     DDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTK
                     TLSGALNGVAGNIDNVAAAAGVLVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARTAAQ
                     NALNSTTAVGSRLMTGALGLVGGVPGLVMLGAA"
BASE COUNT          195 a          221 c          329 g          181 t
ORIGIN      
        1 cagcggacag gcacggcatt ttcagataat gcggccctgt ttgcccgctc ggcagcctca
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggca gaggcgggct cggtgatcac ccagttcagt
      181 caggcgctgg cgcagggtgt attgcgtggt gaggagttta attcggtcaa tgaaagtggt
      241 gaccggatcg tacgtgcact ggctgcgggt atgggcgtgg cccgtaaaga tctgaaggca
      301 atggcggatg acggaaaact gacagcggat aaagtggtcc ccgcgttaat cagccaactg
      361 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccagtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacgaaa
      481 acgctctccg gtgcgctgaa cggtgttgcc ggcaatattg ataatgtggc cgcggcagca
      541 ggtgtgctgg ttgctgtcgg ggttgcacgt tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cacggcgcgg gcaagggcag ccgtgtaccg tgctcagcag
      721 gccgtggcgg cagcccgcgg gacggagatg cagattgcag cagaggcccg tctggcggcc
      781 acacaggaac gcctgaacag aaatattgct gccagaaccg ccgcccagaa tgcgctgaac
      841 agtacaacgg cggtgggctc acgtctgatg actggtgcgc tgggactggt tggaggcgta
      901 cccggactgg tgatgctggg agctgc
//
LOCUS       DAZUFB010000384          912 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZUFB010000384 DAZUFB010000000
VERSION     DAZUFB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.395"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..684
                     /locus_tag="ACHY3M_005520"
     CDS             31..684
                     /locus_tag="ACHY3M_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFS4320179.1"
                     /translation="MPIIKNCLSSISNILRNEKTSYSLIKTEQTGKLLNRKITTTPTP
                     AKLLSYRNADLIKENYITEKVLSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAK
                     VIDWYNPQKNAFGIMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKK
                     YGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(697..>912)
                     /locus_tag="ACHY3M_005521"
     CDS             complement(697..>912)
                     /locus_tag="ACHY3M_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320180.1"
                     /translation="IQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQGE
                     FDFYRQIYPVTGGEHKVEALMWTHLPA"
BASE COUNT          349 a          182 c          146 g          235 t
ORIGIN      
        1 gttcatccat atgaagacaa ggaatttatt atgccaatca taaagaactg cttatcatca
       61 attagtaaca tattacgcaa cgaaaaaaca tcatattctc taataaaaac agagcaaaca
      121 ggaaaactgt taaacagaaa aataaccacc actcccacac cagcgaaact attatcatat
      181 cgaaatgcag atttaataaa agaaaactac attacagaaa aagttttatc tattttcaat
      241 ataaaacggg atttcgttgc tgtaagaatc caaagtaatc agtttactga tttaaaaaac
      301 aagacaattc agggtcataa agacactgtc gcaaaagtca tagactggta taacccacaa
      361 aaaaacgcat ttggtatcat gatgggcaca ccaagacgtt ctgctgatat agcaaaggaa
      421 gaatccagaa acgctctaaa tttcatgata atggaaaagg atacatttaa tgaaaagata
      481 cttaacagca atgctaattt acaaaaaaaa tacggcacaa cagaaaatag tagttgggta
      541 tcggcttctg ttggttctct tctggacaaa ggagccaaag tatatccaga tatatcatgc
      601 tctctgagac tgggagaacc attcattatt acacttccag aaacagtcag gctggatgtt
      661 aatatccacc cactcaaaaa atgatcctga acgtttttat gcaggcaaat gcgtccacat
      721 caaggcttca accttgtgct ctccccctgt cacaggataa atttgcctgt aaaaatcaaa
      781 ctctccctgc tcattaccaa taccaatcag ttgaatgcag ttgccatcta caataaattt
      841 ttccgctgac gtaaccaaaa taaaggcttc cggatgttca ttaggaaagg tgttagcaag
      901 agtacactga at
//
LOCUS       DAZUFB010000385          899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZUFB010000385 DAZUFB010000000
VERSION     DAZUFB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.396"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHY3M_005522"
     CDS             complement(<1..242)
                     /locus_tag="ACHY3M_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320181.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFEL"
BASE COUNT          286 a          200 c          188 g          225 t
ORIGIN      
        1 agttcaaacc aggtcaccac gcctttcacc cggcgctgta cttcatcgcc ctgccatatc
       61 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagcgaa gagagtgact ggttcagatg aaaggaaacc
      181 accgcaaaag catccggcgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct ccgtgaaaat gaataataat ccgcacatat tgcatgtaca acatgtcggg
      301 cggatgagat aagcgagtcg acatctggcg ttaccgggga atatcagaaa aatgtcatgg
      361 ttatgtgatg atttagtctg ggattttact tcagagaaac gggtgtctgt ttagtcaaca
      421 aaataatcac aaacgcaacg gatgggttct cgacgttatt cacgacaggc tcgtacatta
      481 cagcgagtaa taaaaaacct gatatgcaaa gtcaaaccaa tattcataat gtctttttat
      541 tcacaacaat aaataattca ataacaatca ttgaaatgaa tgatggtttg tataaggaat
      601 atacgctacc gccccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
      661 cactagctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatata
      721 ataagcaaat agcatagaaa ataatgacaa ctcccccgag gggaccattt ccaaagcact
      781 gcaccaccac ctggtgccgg atgatcttgc ttcagtggta agagtacctg atgcattgtt
      841 cccaaaatgg tgttttcccc tgcccctcca ataatgtgta gctctcactt gataatcta
//
LOCUS       DAZUFB010000386          898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZUFB010000386 DAZUFB010000000
VERSION     DAZUFB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.397"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..387
                     /locus_tag="ACHY3M_005523"
     CDS             112..387
                     /locus_tag="ACHY3M_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFS4320182.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(467..580)
                     /locus_tag="ACHY3M_005524"
                     /pseudo
     CDS             complement(467..580)
                     /locus_tag="ACHY3M_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(615..>898)
                     /locus_tag="ACHY3M_005525"
     CDS             complement(615..>898)
                     /locus_tag="ACHY3M_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4320183.1"
                     /translation="CHADKILCFSLFGCCLYYDKSIIYTTNAIESLNSVIRHAIKKRK
                     VFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT          314 a          202 c          161 g          221 t
ORIGIN      
        1 tggaaagact atcgtccaaa atactctggt tccacatata aatattctta atgataccaa
       61 cttataacgg aatagcataa aaacactttt catggagcaa aggagaaaac aatgccattt
      121 tcaatcaaaa acagattttc aagttcacaa gtacattacc cggaaatatc cggtcccata
      181 aaagacaagc cagcgtcaaa gaactgcata cttacatcaa caacatgtaa tgtagatagc
      241 tatacagtgt accaaaaaaa agcctgtagt tttgacatgc gcccacccgg cgcaggagaa
      301 agaaccccaa aactaaaact ctcagttact gagatgacat ggctatctaa aactatagaa
      361 acagagatac acaacacaaa agaatagcaa ccacttacca gagaacacaa aaagccacag
      421 acccgaaaca cctgactgca agcaaccacc tccacaggag gtggttttac catgaacttc
      481 ctgtgtactg acttgtgttc ataataatat ttttgtgttt aaactcaata aagtcacaaa
      541 aatgattgta atcatgcaat gtagtaaaat taaaatattt gaccctgtac acgattctgt
      601 gtaaatgcct tttctcagaa gtgaccgtcc aggcggtcac cgaactcgat aataaagcgg
      661 ctcattgcca tacgccagtc ccgcagtggc atcgtccatt tctgtgaagc cgcctggatt
      721 gccagccaca cgaccttttt cactgagtca tccgtcggga acaccttgcg ttttttgatg
      781 gcatgccgga ttacgctgtt cagcgactcg atggcgttgg ttgtgtagat gatggattta
      841 tcgtaataaa gacaacagcc aaataatgaa aaacataaaa ttttatctgc atggcaaa
//
LOCUS       DAZUFB010000387          888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZUFB010000387 DAZUFB010000000
VERSION     DAZUFB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.398"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..859
                     /locus_tag="ACHY3M_005526"
     CDS             <1..859
                     /locus_tag="ACHY3M_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFS4320184.1"
                     /translation="GAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLAL
                     PENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIK
                     GYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIY
                     IKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHH
                     KLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPP
                     MKNIMDHTK"
BASE COUNT          346 a          157 c          143 g          242 t
ORIGIN      
        1 aggcgctttt ttatcggcta ttcatcacca gatcattgaa aaccaactgg atacagaaac
       61 aataactatt agttatgaat ttatcctctg tcaacatctt atcgcaaata ccgagtttag
      121 ctatttggca ctccctgaaa attataaccg tttgttttta ccaaattcaa aaaaccaaac
      181 aaataatcgt ttcaaaacgt taaacagcaa agctattggc aggctacttg ctgctggtgg
      241 cgtatacaat gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc
      301 aataaaaggc tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc
      361 atctattctt ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt
      421 aggcaaactt agaggtcaac aaaaacttct tgatggtata gacataatag aaataatata
      481 cattaagaga ccttcaaaag acttagctaa cttacgaaag gagtttaata aaactgtaag
      541 aaaaaatttt cttatcaaac ttgcaaaaac ctccgaagca tctggaagat tcaacgccga
      601 agacctttta agaatgagaa agggcaatgt tcctctaaat tataatgttc accataaact
      661 atctctagat gatggtggta ctaatgattt cgaaaattta gtattaatcg aaaacgaacc
      721 atatcataaa gtttttacta acatgcaatc acgaatagct aagggaatat tagtaggtga
      781 aagcaaaatc actccctggg ccattccatc tggctcaatt tatcctccca tgaaaaatat
      841 tatggaccac acaaaatgat gactaaaacc caaataaata aattaata
//
LOCUS       DAZUFB010000388          886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZUFB010000388 DAZUFB010000000
VERSION     DAZUFB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.399"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..675
                     /gene="rfaY"
                     /locus_tag="ACHY3M_005527"
     CDS             <1..675
                     /gene="rfaY"
                     /locus_tag="ACHY3M_005527"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFS4320185.1"
                     /translation="VFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARG
                     PLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLN
                     FAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEK
                     GLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVK
                     LGLKSK"
     gene            700..>886
                     /locus_tag="ACHY3M_005528"
     CDS             700..>886
                     /locus_tag="ACHY3M_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4320186.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHI"
BASE COUNT          327 a          123 c          157 g          279 t
ORIGIN      
        1 gttttcataa aagataatga tcccttttat gagcaagttc taaatgactt tttaacttgc
       61 agggttaaaa cattaaaagt ttttcgttcg attgatgata ctaaagtcat tcttattgac
      121 actgctcgtg gacctcttgt acttaaagtt tatgcgccaa agcataaaat gaccgaacgt
      181 ttcttaaaat cttgcataaa aaaagattat tatgaaaatt taatttatca aaccgatcgg
      241 gtacgtggtg aaggcatcca atcaattaat gactattttt tgctagctga acgtaaaaca
      301 ttaaatttcg ctcattatta tattatgctc attgagtata ttgaaggtgt cgggttaaat
      361 gagtatttgg aaatttctga agatctgaag gatcaattat cagaatcgat aaaagagttg
      421 catcagcatg gaatggtgtc tggcgatcct cataaaggaa attttatagt atccgaaaaa
      481 ggtttaagac taattgacct ttcaggtaaa aaaacaaccg ctgttttaaa agcaaaagat
      541 cgcattgatc ttgaacggca ctataatata aaaaatgaat tgaaagactt tggctataca
      601 tatcttatat tcaaaaagaa aattaaaaaa gtaatcaggg atgtaaaagt caaactagga
      661 ttgaagagca aataatgatt atgatgaagg gtaattaaat tggattttaa acatcttact
      721 caatttaaag atataattga actggacaag cgccccgtta aacttgatga acgggaaacg
      781 tttaatgtct catggggtat tgatgagaac taccaggttg gggctgcgat ttcaattgct
      841 tcaattcttg aaaataataa acaaaacaaa tttacctttc acataa
//
LOCUS       DAZUFB010000389          883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZUFB010000389 DAZUFB010000000
VERSION     DAZUFB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.400"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..481)
                     /locus_tag="ACHY3M_005529"
     CDS             complement(<1..481)
                     /locus_tag="ACHY3M_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4320187.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGKAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     "
     gene            complement(481..822)
                     /locus_tag="ACHY3M_005530"
     CDS             complement(481..822)
                     /locus_tag="ACHY3M_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4320188.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRSTGLNPQL
                     KTFQAVFRVTDEATRCWLDEFLSWHGGYRAFLWRPPKHNRMVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
BASE COUNT          184 a          326 c          187 g          186 t
ORIGIN      
        1 ccccgtccgt ctccgtcggt gttgccagca caaacgaggc tgtcatggcc gtcagctctg
       61 acatctgctc caccacccag cggtcgctca gctcctgctc cgggtccgct tccggattgc
      121 ccgccacaaa attcaccgca tccagaaaac gggcatacac ccggcggcgg accaccgtgg
      181 cccccaccag gctctgcagg tcctccgcca tcccggtgac cagaccaaac agattggaca
      241 ccgtcagcga cggtctggca ctgctgccct tcccgttcat ctcaaaaccg ctgccgtcaa
      301 tcgggtatac ctgatattgc cgcccctgcc aggtgaccgc cttccctttt tcattcagct
      361 cattacagaa aaaataccgc tcaccaccct gtaccgtcag gtcgatttcc cagagtacca
      421 cccgcggtga ctgctctgac ttaaccgact cgttcagact ttcttcgcga atatcctgca
      481 tcagttcacc acctgctcta tcgtacagct gaaatcactg taccggacgt tatccgtgac
      541 gctccactcc cggcatacca ccctgaccat ccggttatgt ttcggcggtc gccacaaaaa
      601 ggcacggtaa ccaccatgcc acgataaaaa ttcatccagc cagcaccggg tcgcctcatc
      661 cgtcacccgg aataccgcct gaaatgtctt cagttgagga ttcagccctg tggagcggcg
      721 ctgttcataa ccgtcaccaa accgtaccct cgccaccgac ggtttctcac tcacctgcat
      781 cccttcacgc gggaccagat gcagcgtttt tatctcagcc actcagcatt cctccgtcac
      841 gtcgcatgga caacatcacc gcctgcaccc gctggtcaat cag
//
LOCUS       DAZUFB010000390          881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZUFB010000390 DAZUFB010000000
VERSION     DAZUFB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.401"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHY3M_005531"
     CDS             complement(<1..191)
                     /locus_tag="ACHY3M_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFS4320189.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKL"
     gene            complement(166..732)
                     /locus_tag="ACHY3M_005532"
     CDS             complement(166..732)
                     /locus_tag="ACHY3M_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4320190.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          221 a          212 c          142 g          306 t
ORIGIN      
        1 agtttatctt tctgtttctg ctcctctcgt cgtagtttct tctctgctgc tttttccgct
       61 ttttcgcgtt ctttacttcg tcgttcgagt gctatcttgg ttccacaaat ctcattacac
      121 caatattgat tttgatattt tggtataaac cattcattgc aacatttaca tttccttcga
      181 tagattcgca taagtgctcc tttcgttgcc ggaaaaatca ccgtaatact tatctcgggc
      241 ttcttcagca actagtaccg ctaactccag atcatcaaag catccgaagt gtttactctt
      301 gccatggaat cctagcctaa cattccattt tttctgtcgt ttgtgccaag taacccctct
      361 gcaacctgat ttgctattct ttcggatcct tatatttctt gaattttcta ttggcaggca
      421 ttctcttaaa ttttctggcc tattgtcggt cctaattcca ttaacgtggt caatttgacc
      481 agcaggccaa cgattatgag ttatgtaaaa aactaagacg tgagttttat atctacgccc
      541 atctatcatg atcattgaat aaccgttgga atcaaaagtt ccagcaacac tatttaatgc
      601 tatccttccc tgagtgggaa ctttccatct aaataccccg gtagatttat cgaaacttag
      661 taactcaaat atccttttaa cagttaaatc ttctctttta cggttacatc gtcttcgcgc
      721 tggtttagcc atcgccttct tcctccgtaa tggttttctg aatttggcca cctgaacaga
      781 gctcaccaaa gctatggatg tcggtatttc cacaatacca agatggcgaa aataactgca
      841 tgataagcct cagggaaaag ggaagacact acccccgata a
//
LOCUS       DAZUFB010000391          877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZUFB010000391 DAZUFB010000000
VERSION     DAZUFB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.402"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>877
                     /locus_tag="ACHY3M_005533"
     CDS             <1..>877
                     /locus_tag="ACHY3M_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFS4320191.1"
                     /translation="ATGRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTAW
                     PDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQ
                     HGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTD
                     TTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGL
                     LDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLA
                     LISEDGNTAWSAEYDE"
BASE COUNT          199 a          250 c          305 g          123 t
ORIGIN      
        1 gccacgggca ggctggagag cgtgcgcacc ctcgcaccgg gcctggacat ccgcatcccg
       61 tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctacaccc ggacagcacg
      121 ctcacggcgt ggccggataa ccgcatcgca gaggatgcgc actatgttta ccactatgat
      181 gaatacggca gactgacgga gaagacggac cgcatcccga cgggtgtgat acggacggac
      241 gacgagcgga cccaccacta ccactacgac agccagcacc gcctggtgtt ccacacgcgg
      301 atacagcatg gcgagccact ggtcgagagc cgctacctct acgacccgct gggacggcga
      361 atggcgaaac gggtatggcg gcgggagcgt gacctgacgg ggtggatgtc gctgtcgcgt
      421 aaaccggagg agacatggta cggctgggac ggcgacaggc tgacgacggt acagaccgac
      481 accacgcgta tccagacggt ataccagccg gggagcttcg cgccgctcat ccgcatcgaa
      541 acagacaacg gtgaacggga gaaagcgcag tgccgcagcc tggcggagaa gatacagcag
      601 gaagggagcg aagacggtca cggtgtggtg ttcccggctg aactggtggg gctgctggac
      661 aggctggagg gggaaatccg ggcaaactgc gtgagcagtg aaagccgcca gtggcttgcg
      721 cagtgcgggc tgacggtgga gcggctggcg gcacagatag agccggttta tttgcctgag
      781 cgaaaaatac atctttatca ctgtgaccac cggggcctgc cgctggcgct tatcagtgaa
      841 gacggcaata cggcgtggag cgcggaatat gatgaat
//
LOCUS       DAZUFB010000392          871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZUFB010000392 DAZUFB010000000
VERSION     DAZUFB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.403"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..871
                     /locus_tag="ACHY3M_005534"
                     /pseudo
     CDS             16..871
                     /locus_tag="ACHY3M_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          225 a          229 c          239 g          178 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga t
//
LOCUS       DAZUFB010000393          848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZUFB010000393 DAZUFB010000000
VERSION     DAZUFB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.404"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>848
                     /locus_tag="ACHY3M_005535"
     CDS             <1..>848
                     /locus_tag="ACHY3M_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320192.1"
                     /translation="YFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTIN
                     CVVEIENIVFGGYIYTLNNGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSGKTE
                     SVHRTINKLNYEKLPFGFIIAAFAILKIAFKIKFSKNHVNIRALLNDINYFMTYQGES
                     INLISLDHEYPESCLQNDTNTYLLGRVIFLYNSMIYKFINCQEHETNNIHSAMINNLL
                     QEVDIALGKINDIIDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVG
                     MITFLI"
BASE COUNT          308 a          152 c          126 g          262 t
ORIGIN      
        1 attactttcc taatgagaac tcaccatttt atacactgaa aattgacagt gcattatcgt
       61 gggatgccat tgacgcaata cgagactata gtatttctga tatttatctt tatcctgaac
      121 aaaaaacaat aaattgtgtc gttgagattg aaaacattgt ctttggcggt tacatttata
      181 cattgaacaa cggcgtcaca ttacaaaaca tagaaaactc tttaaaagat tcttcatgcc
      241 attatgtctt aaatggctat acagaatttg ttaactgttt gagacaactt acttcaggaa
      301 agactgaaag tgttcatcgc accatcaata aactgaacta tgagaaatta ccttttggat
      361 ttatcattgc cgcgtttgct atactaaaga tagcatttaa aataaaattc agtaaaaatc
      421 atgtaaatat ccgagcatta ttaaatgaca tcaattattt tatgacttat cagggcgagt
      481 ccattaacct tatttcactg gatcacgaat acccagagtc ctgtcttcaa aatgacacaa
      541 acacatattt attaggaaga gtaatatttc tgtataactc aatgatttat aagttcataa
      601 actgtcagga acatgaaacc aataacattc actcagctat gataaataac ctattacagg
      661 aagttgatat agcccttggt aaaataaatg acattataga cagcagaaac atatcagccc
      721 cccatgaact ggcaaatatt cttacccgcg aaaaaatact tacaacacgg gaaaaaaaag
      781 gaaacctgat aagcctgttt gatggattca ctttattcca ttgtgttgga atgataacct
      841 ttcttatc
//
LOCUS       DAZUFB010000394          845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZUFB010000394 DAZUFB010000000
VERSION     DAZUFB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.405"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..257)
                     /locus_tag="ACHY3M_005536"
     CDS             complement(<1..257)
                     /locus_tag="ACHY3M_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFS4320193.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFIS"
BASE COUNT          264 a          149 c          174 g          258 t
ORIGIN      
        1 gaaataaaat gctcttcaat taataatcca tcaatataaa caccttgcat atctgagcaa
       61 gattttgaga ctttctttcc gaactctata agagtctcgt tataactctt taaggcaata
      121 cctgaatcag ggtcaattgg cactggttca atttcgagta atggcaaaat ttttgatttc
      181 ttttcaatgg atagctgcga taaagctgat aactcagaac gtttcgcttt cagaatagga
      241 atatatgaaa ttgtcatgtt aataccttat acagaatcaa ttaacaatta atggttcttc
      301 ggacattgaa atttatatcg caaaaattgg caaactctcc tcgactactc acttgtgggt
      361 atcctggcgt aaaaggaatg acttggcaat acccggtgtg atcataagtc attgaaaatg
      421 atcattttta tcagtctttc ttccatgatg aatgctaatg ccatttgatt tgttgaggtg
      481 aaaactgtta aaaatcaaaa cgatgtaatt gaaatgaacg ttcggtagca ttcacgcttt
      541 aaatgtttct tttgtgctga ttggatgaat ttgggtcact tctgatgaga gatgttgcag
      601 gaaaagaagt tggcattgat ctattggata gttagaattg ctgcgggtgc ttgaggctat
      661 ctgcctcagg catgaacacc aaaaggcaga tagagaaaag ccccagttaa cattacgcgt
      721 ccggcaagac gcttaacatt aatctgaggc caatttcatg ctttgcacat gtaggttagc
      781 ctcttacgtg ccgaaaggca aggagaagca ggctatgaag cagcaaaagg cgatgttaat
      841 cgccc
//
LOCUS       DAZUFB010000395          835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZUFB010000395 DAZUFB010000000
VERSION     DAZUFB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.406"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>835
                     /locus_tag="ACHY3M_005537"
     CDS             <1..>835
                     /locus_tag="ACHY3M_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS4320194.1"
                     /translation="QTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSA
                     TGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSR
                     YGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETR
                     YEYNIAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENG
                     SHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRT
                     VK"
BASE COUNT          206 a          252 c          241 g          136 t
ORIGIN      
        1 cagacggatg ccgcaggcag gacaacagag tacagcccgg atgtggtgac gggactcatc
       61 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacaaccag
      121 ttaacgtcag ccaccgggcc tgacgggctg gaattgcgcc gggaatatga tgaatccggc
      181 cgtctgattc aggaaactgc ccctgacggc gatatcaccc gctaccgtta tgataatcca
      241 cacagtgact taccctgcgc aacggaagat gccaccggca gccggaaaac catgacgtgg
      301 agccgttacg gccagttgct gaccttcact gactgttccg gttatgtaac ccgctatgac
      361 catgaccgtt ttggtcaggt gacggcggtg caccgcgagg aaggcctgag ccagtaccac
      421 gcatacgaca gccgtggaca gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg
      481 tatgaataca acatcgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      541 gggacacagt acgatgcgtg gggaaaggcc atctgtacca cgcagggcgg gctgacgcgc
      601 agtatggaat acgatgctgc cggacgggtc atccgcctga ccagtgaaaa cggcagccac
      661 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      721 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      781 gtcacccact ggtactatga cgaagcagac cgcctcactc accgcaccgt gaagg
//
LOCUS       DAZUFB010000396          835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZUFB010000396 DAZUFB010000000
VERSION     DAZUFB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.407"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..192
                     /locus_tag="ACHY3M_005538"
     CDS             <1..192
                     /locus_tag="ACHY3M_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320195.1"
                     /translation="EASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGSASGG"
     gene            237..458
                     /locus_tag="ACHY3M_005539"
     CDS             237..458
                     /locus_tag="ACHY3M_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320196.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            404..>835
                     /locus_tag="ACHY3M_005540"
     CDS             404..>835
                     /locus_tag="ACHY3M_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320197.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVE"
BASE COUNT          176 a          217 c          283 g          159 t
ORIGIN      
        1 gaggcatcac tggaaatgca gtctgagccg ggcggcgaca gtaccacgcc gtccccggtg
       61 gagctggttt ccatgttcca gacaggcagc gtggccatcc gtgcggagcg ctggatcaac
      121 tggcgtcgtc gccgtactgc ggcggtggcg gtgatcaccg gagtgaacta cggcagtgcg
      181 tccggcggct gagtctgata aggaggacgg gaggcgtgcg cctcccgtaa caggttatgg
      241 caaagatccg atatctgcag ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg
      301 cacagccgtg tgcggaggtg ctggttcgcc tggggaaggc ggagtacatc acggtgcgac
      361 gtccggcagg tcagaaaaag aaacgtgatg cggagcatgg cgaatgtgga accttttacg
      421 gcgaacccga aaaaaccaga aatcaggacg tgacgtaaga gaggcgggct ggaccagcct
      481 gtttcaggcg gtggctgagc ccttttccgg cgcctggcag cagggcgtga aagccgatcc
      541 tgaagccgtc ctctcctttc atgcggtgtt tgcatgtatt tcgctgatat cccaggatat
      601 cgccaaaatg cggctgcgtc ttatgcagac ggatgcgcat gggatacgca gggaaacgcg
      661 ccggggggat attgcccgcc tctgtcgtcg tcccaacgcc cagcagaacc gcatccagtt
      721 ttttgaactg tggctgaacg ccaaactgcg tcatggcaat acggtggtgc tgaaaatccg
      781 taatgcccgg gggcagatca aagaactgcg tattctggac tggagccggg ttgaa
//
LOCUS       DAZUFB010000397          830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZUFB010000397 DAZUFB010000000
VERSION     DAZUFB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.408"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(536..>830)
                     /locus_tag="ACHY3M_005541"
     CDS             complement(536..>830)
                     /locus_tag="ACHY3M_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4320198.1"
                     /translation="NLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDD
                     VRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          261 a          168 c          141 g          260 t
ORIGIN      
        1 gcaatagaaa cagttcagat aaagataggg atctacttca caatttaaat gagaaactaa
       61 aacttacatc ttgaaataat ctcattgatt ggatgaatat ttatagtgca gtgacatcat
      121 ttttaaataa tagttcaaat aaaagggctc acgatgaaaa aattaacagt ggcaatttct
      181 gctgtagttg catcttaacg tataaaccag atttaataac aacgaactag atttaataac
      241 aacgatatcc gtacctatcc ggatatctaa tgtcataagc atccctttaa ttatcccctc
      301 aacctcttgt aactttccca ctcttgaact ccacatagtc caaacgccgg atgcaaaaaa
      361 atcacacgtg catttctgaa attgtgatgg aggacgatag tatcaattat atgtgcttat
      421 aaacgtcccc gattagcgca gaagtcaccg gattgttcag gctccaatag gttgagtatg
      481 ccgtatttat tttccaaaat aaaaatcgat gctgttgctc cttcataaaa tacttttact
      541 ctctggacgc ttttcttagc tctcttggtg ttatcttaaa acgattatgg aacctttcag
      601 taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg ggttttactg
      661 tcgtttgtat agcagacaac gcattattta acctcacgtc gtccaatata ctttgaaaac
      721 ttactccctc gcttgccaga cggcgatgca atgtggaaac agaaatgtag agatatctcg
      781 ctaccttgtt tgctgtccat tttgttccag gttcggatag cattaggtta
//
LOCUS       DAZUFB010000398          827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZUFB010000398 DAZUFB010000000
VERSION     DAZUFB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.409"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            468..683
                     /locus_tag="ACHY3M_005542"
     CDS             468..683
                     /locus_tag="ACHY3M_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4320199.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAAKGE"
     gene            683..>827
                     /locus_tag="ACHY3M_005543"
     CDS             683..>827
                     /locus_tag="ACHY3M_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4320200.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGV"
BASE COUNT          218 a          160 c          220 g          229 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc
      661 gtaaggctgc gaaaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat
      721 tggtggtggg gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg
      781 tctggaaggt gtcagctaca taccatacaa agatatcgtt ggcgtat
//
LOCUS       DAZUFB010000399          817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZUFB010000399 DAZUFB010000000
VERSION     DAZUFB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.410"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..817
                     /locus_tag="ACHY3M_005544"
                     /pseudo
     CDS             1..817
                     /locus_tag="ACHY3M_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          213 a          196 c          253 g          155 t
ORIGIN      
        1 acggaagaaa atgcgaaaaa actgaagagc aactgggaca gcgggtatac aggcgaaaat
       61 gcggggaaaa cggccattct gagcaacggg gcaaaataca accccacgac gttttcacct
      121 gtggatgcgc agacggtgga acaactgaag atgaccgctg aaattgtctg ttcggtgttc
      181 cgtgtcccgg cctacaagat tggcgtggga caaccgcctt ccagtgacaa cgtggaggcg
      241 ctggagcagc agtattattc ccagtgcctg cagacgctga ttgagtccat tgaactgtta
      301 ctgtatgagg cgctggaaac aggggaaaac gagagcacgg agtttgacgt caccacgctg
      361 ctgagaatgg acagcgaacg tcgcatgaaa acgctgggtg aatcggtgaa aaatacgctt
      421 ctcacgccca atgaggcccg taaacgtgag aacctgccgc cccttgccgg cggtgatgca
      481 ctgtatcttc agcagcagaa ctacagtctg gaggcgctgt cccgtcgtga tgcccgtgag
      541 gatccgttcg cgtcttccgg gaaaacgtcg tcaggcacac gccaggaggg cgcgtctgac
      601 ggtaataagg ccataagcga aacagagcgt gatgcggtga aggcgatgtt cagggggata
      661 ctgcgaaaat gacagagcgt gaactgtcca ttatccgtgc gctgggtgaa gaattctcca
      721 cggtgctggc ggatttacag cgcacatttg aggggaagat ggcctcgcag gcacaagcgt
      781 ttgaagagaa actggcttcc ctgtcggcgg tattaca
//
LOCUS       DAZUFB010000400          816 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZUFB010000400 DAZUFB010000000
VERSION     DAZUFB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.411"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..105)
                     /gene="gpU"
                     /locus_tag="ACHY3M_005545"
     CDS             complement(<1..105)
                     /gene="gpU"
                     /locus_tag="ACHY3M_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFS4320201.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQ"
     gene            complement(118..741)
                     /locus_tag="ACHY3M_005546"
     CDS             complement(118..741)
                     /locus_tag="ACHY3M_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4320202.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     TEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(744..>816)
                     /locus_tag="ACHY3M_005547"
     CDS             complement(744..>816)
                     /locus_tag="ACHY3M_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS4320203.1"
                     /translation="GRTLTVTRYDTYNGKPRIFVEQE"
BASE COUNT          155 a          255 c          208 g          198 t
ORIGIN      
        1 ctgttcatca ataaaaaccg ggcgaccgtc aaaccagctc accctctcag aaatattttc
       61 tttcagggca gacagaactg ccgcccgtat ttcacggtgt ttcatacacc ctcccagtca
      121 ttttcttttc agcaccagac gtaactgatg cgtcatggct ttcatcatct gcgccggtaa
      181 tttttcccgg tacatccggt cccgttcacg ttcaaaggtt tctgccagcg gtccggcagt
      241 cggaatcttc accacttcag tcggcagacg gtggcgtttc ggcctccctt tgctgtcagc
      301 gccggtggac gatgatgccc acggcatacg ctgcatcaca tgccagcgtc cgttagccag
      361 ccgggtgata aaggcatccg ggatccgtct tttccccaca atcagcacac tgccaccgcc
      421 tttcgtgacc gaacgctcgc ctttctttcg tcgctttctg cgggaaagcc gcacgctggc
      481 cgtccccagt tttatggcgg gcagattacc ggtgttgatg atgaccttcg cataaaccct
      541 gtctgcgctg gcccgttgca ggcggatacg ctcacggatg agacggcgcg gaaccgccag
      601 ctccctggca accgaagagg ccgttttcgc gatgatggac tccgccacac ggttaagtgt
      661 cgtggcggca gcccggggaa cggcacggcg gtcaattgca tccagatttt tcatggcctg
      721 cgccagacct tttattgcca tactcattcc tgttcgacaa aaatccgggg tttaccgttg
      781 tacgtgtcat aacgggtcac cgtcagtgtg cggccc
//
LOCUS       DAZUFB010000401          805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZUFB010000401 DAZUFB010000000
VERSION     DAZUFB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.412"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..786
                     /gene="cif"
                     /locus_tag="ACHY3M_005548"
     CDS             <1..786
                     /gene="cif"
                     /locus_tag="ACHY3M_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFS4320204.1"
                     /translation="LTGAISVNTEAVLSPMQHTSALHVRDFASLCSQNLKANVLLNSD
                     DHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGRENGIVEPSCGVTANAI
                     MKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVNDGALGHAYIVDIPKG
                     ENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNYFSCMSQNKTDLEQI
                     ATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTLR"
BASE COUNT          252 a          145 c          174 g          234 t
ORIGIN      
        1 ctgacagggg ccatatctgt aaatactgaa gctgtattat ctcccatgca acacacttca
       61 gccttacatg taagagattt tgcttccctg tgctcacaga acctcaaagc taatgtattg
      121 ctaaatagtg atgaccacga agtacctata catcagaaaa atcctgctgc aataatgcaa
      181 aatatcgact ctaacatcaa acagatggca acagactggg ggatgtcgat tgaggaggtt
      241 gaggttatta tagggcgaga gaacggcatt gtggaaccct cctgcggagt taccgctaat
      301 gctattatga aactatttct ggacaaggat ggcttcagtt actgctttga aaatgaacag
      361 acactatcgc tcgagcagct tcaggagcgc ctgtcctgta tgcctgaatg taagagcttt
      421 gtattacgtg ttaatgatgg tgcgcttggt catgcttaca ttgtcgatat tcccaaagga
      481 gaaaactctt gtcgtcctgc attcttgtat cagtcagatt taggagaggg cgtcaccaga
      541 aagttaagat ttgaggactg gatgacgcat aaagcattga ctccgatttt gctggatgat
      601 atttgtaatt acttctcctg catgtctcaa aataagacag atttggagca gattgcaacg
      661 ttatttgata ttgatggaaa tgttaaaatg ttacgaaagg aaaatattca atatcaaaag
      721 catgacaatt ttagtttcca gttgtttgag tatgacaccg ataatattga aaaaacattg
      781 agataataaa atcactatgt agtta
//
LOCUS       DAZUFB010000402          796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZUFB010000402 DAZUFB010000000
VERSION     DAZUFB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.413"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..243
                     /locus_tag="ACHY3M_005549"
     CDS             <1..243
                     /locus_tag="ACHY3M_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320205.1"
                     /translation="PDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQ
                     TGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
     gene            288..509
                     /locus_tag="ACHY3M_005550"
     CDS             288..509
                     /locus_tag="ACHY3M_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320206.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            455..>796
                     /locus_tag="ACHY3M_005551"
     CDS             455..>796
                     /locus_tag="ACHY3M_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320207.1"
                     /translation="MWNLLRRTRKNQKSGRDVREVGWRSLFQAVAEPFAGAWQQGVKA
                     DPETVLSFHAVFSCISLISQDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQN
                     RIQFFELWLNSK"
BASE COUNT          169 a          200 c          269 g          158 t
ORIGIN      
        1 ccggatattt atctggcgga tgacggcggc gtggcagtgg atatgtcccg tgaagcatca
       61 ctggaaatgc agtctgagcc gaccggcgac agtaccacgc cgtccccggt ggagctggtt
      121 tccatgttcc agacaggcag cgtggccatc cgtgcggagc gctggatcaa ctggcgtcgt
      181 cgccgtaccg cggcggtggc ggtgatcacc ggagtgaact acggcagtgc gtccggcggc
      241 tgagtctgat gaggagggcg ggaggcgcga gcttcccgca gtaacagatg gcaaaaatcc
      301 agtatctgca gggcacgcat gatgcccggg ccggggatat ccgtgatgtg gcacagccgt
      361 gtgcggaggt gctggttcgc ctggggaagg cggagtacat cacagcgcga cgtccggcag
      421 gtcagaaaaa gaaacgtgat gcggagcatg gcgaatgtgg aaccttttgc ggcgaacccg
      481 aaaaaaccag aaatcaggac gtgacgtaag agaagtgggc tggcggtccc tgtttcaggc
      541 ggtggctgag ccttttgccg gtgcctggca gcagggggtg aaagccgatc cggaaaccgt
      601 tttgtccttt cacgcggtgt tttcatgcat ttcgctgata tcccaggata ttgccaaaat
      661 gcggcttcgc ctgatgcaga ctgatgtaca gggaatacgc cgggaaaagc ggcagggaga
      721 tactgcccgt ctctgtcgtc gtcctaatgc gcagcagaat cgcatccagt tttttgaact
      781 gtggctgaat tccaaa
//
LOCUS       DAZUFB010000403          795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZUFB010000403 DAZUFB010000000
VERSION     DAZUFB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.414"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..549
                     /gene="escU"
                     /locus_tag="ACHY3M_005552"
     CDS             <1..549
                     /gene="escU"
                     /locus_tag="ACHY3M_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFS4320208.1"
                     /translation="FANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKH
                     EGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTH
                     IAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARSLYKNIHKGQ
                     YITEDFFEPVAQLIRIAIDLDY"
     gene            complement(546..>795)
                     /gene="etgA"
                     /locus_tag="ACHY3M_005553"
     CDS             complement(546..>795)
                     /gene="etgA"
                     /locus_tag="ACHY3M_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFS4320209.1"
                     /translation="LDLLSKFNISEDDLLNNACINISVAGYILASNIKSRGNTWDAVG
                     AYNAGYFNTPNAVELRRQYAMKIYKTYNKLKNNEQIID"
BASE COUNT          254 a          135 c          140 g          266 t
ORIGIN      
        1 tttgctaatt tcacaggact gaacgcatat caagctcttg tcgttgttgc cttttttgtt
       61 tttcttttat ggaaaggcgt gctattcgga tatctactct tttcagtatt tgatttctgg
      121 ttccagaagc atgagggact gaagaaaatg aaaatgagta aagatgaggt gaaacgagaa
      181 gccaaggata ctgatggtaa ccctgaaatt aaaggggagc gccgtcgcct tcattccgag
      241 atacaaagtg gaagtttggc taataacatc aaaaaatcaa ccgttattgt taaaaacccg
      301 actcacattg cgatttgcct atactataaa cttggggaga ctccattacc tttagttatt
      361 gaaacaggaa aagatgccaa agctctacag atcattaaac tggctgaact ctatgatatt
      421 ccagtgattg aagatattcc tttagcaaga agtctctata agaatataca taaaggacaa
      481 tatataacag aagacttttt tgaacctgtg gcacaattga ttcgtattgc gatagacctt
      541 gattattaat cgataatttg ctcattattc tttaatttat tatatgtttt atatattttc
      601 attgcgtatt gccttctgag ttctactgca tttggtgtat taaagtaacc tgcattatac
      661 gcaccaactg catcccatgt attccctctg gattttatgt tagaggctaa gatataacca
      721 gcaacagata tgtttataca ggcattattc aataaatcat cttcagatat attaaatttg
      781 cttaataaat ctaaa
//
LOCUS       DAZUFB010000404          792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZUFB010000404 DAZUFB010000000
VERSION     DAZUFB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.415"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>792
                     /locus_tag="ACHY3M_005554"
     CDS             33..>792
                     /locus_tag="ACHY3M_005554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFS4320210.1"
                     /translation="MAIGSLAEAANQKGDFAQNAAKNAGVNLAAQGVLSAAAKGIGRG
                     ITAVRGEISPADQQLLKRAAAADVPVMTSDVVPPKTKLGNQLQGYSEGVIAGTGPMRA
                     AQQDARTKLVNRFTEKYGDYDPSVVVDSLKSGVAREKSLAKSKLNNLSGRMVGKPVDT
                     SGAIRAIDGAVNELGKLKGVSDTQTISALNDYKNAIQEITNGDDAFELLDKLRTQFRI
                     DVKGDRTVLPSMSQTMVDRVYNSLTNSLSKSIAKG"
BASE COUNT          249 a          169 c          197 g          177 t
ORIGIN      
        1 atttagttcc aggagttgga acggcaggaa gcatggctat tggatcactg gcagaggccg
       61 caaatcagaa aggtgatttc gcacaaaatg cagctaaaaa tgccggagtt aaccttgccg
      121 ctcagggtgt tctttccgca gcagcaaagg gaatagggcg tggaattact gctgttcgtg
      181 gcgaaatatc accagcagat cagcaattgc tcaagcgtgc cgctgcggca gatgtaccag
      241 ttatgacatc ggatgtagtt cctccaaaaa caaaacttgg caatcaactg cagggttact
      301 cagaaggagt catagctggt actggaccaa tgagagccgc acagcaggat gccagaacca
      361 agcttgttaa tcgcttcacc gaaaaatacg gcgactacga tccatctgta gtcgttgata
      421 gtctaaagtc aggcgttgca agggaaaaat cgttagccaa gtcaaaacta aacaacctgt
      481 caggaagaat ggttggaaag ccagttgata caagtggcgc cataagagcc atcgacggag
      541 cagtaaacga acttgggaaa cttaaaggtg tttctgacac ccagaccatt tctgcgctta
      601 atgattataa gaatgccatt caggagataa caaatggaga tgatgccttt gagttacttg
      661 ataagctgag aactcagttc cgcattgacg taaaaggcga tcgtacagtt ctgccatcaa
      721 tgtctcaaac aatggtcgac agggtctata actcgctaac caatagcctt agtaaatcta
      781 tagcgaaagg ac
//
LOCUS       DAZUFB010000405          791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZUFB010000405 DAZUFB010000000
VERSION     DAZUFB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.416"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..767
                     /locus_tag="ACHY3M_005555"
     CDS             51..767
                     /locus_tag="ACHY3M_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4320211.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKVIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          262 a          136 c          168 g          225 t
ORIGIN      
        1 tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta
       61 aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg
      121 caaaattcgg tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa
      181 acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta
      241 ttgataaagc aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt
      301 atgaaggctt tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta
      361 accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg
      421 caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggatcagacc
      481 ctgaccatat ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag
      541 aagaaggtaa cattgttatt tataccgaac ctactgacct tcataaagta atcgcggctc
      601 taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg
      661 aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag
      721 atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tattttaaag
      781 aaatctgtct t
//
LOCUS       DAZUFB010000406          786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZUFB010000406 DAZUFB010000000
VERSION     DAZUFB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.417"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..439
                     /locus_tag="ACHY3M_005556"
     CDS             <1..439
                     /locus_tag="ACHY3M_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4320212.1"
                     /translation="LGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALW
                     GSALAAGGFSATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNM
                     IAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
BASE COUNT          193 a          169 c          256 g          168 t
ORIGIN      
        1 gctgggtgag ggaacggtca tccacattgt gccgcgactg gccggggccg gaaagggtgg
       61 actgcagatt gtgctggggg cggcagccat cgtgggctct ttcttcactg ccggggcatc
      121 gatggcgtta tggggttcag ccctggcagc cggtggtttt tctgccacca cgatgctgtt
      181 ttcactgggt gccagcatga tactgggtgg tgtggcacag atgctggccc cgaagccaaa
      241 aacaccggaa tacagggcaa cggataacgg taaacagaac acgtactttt cgtcgctgga
      301 taacatgatt gcccagggga acccgatgcc ggtgccttac ggtgaaatgc tggttggctc
      361 acggcgaatc tcccaggaca tcagtacccg tgatgaaggc ggtgacggga aggtggtggt
      421 tatcgggcgg caggggtaaa gcataaaaaa atcccgcagt gtatggaggc tgcgggaaca
      481 gaaaatgaag attaaccaca gggagttttg tttttattgg cccgaaaaaa ctgtaacgcc
      541 cgggaatgat atctgccacg ggggcgtaca gaaaatgtga agaaattcag aaattttatt
      601 ccgtcatgac acaggcaccc tccgggatgc ctgttgtttt tgtgcgtaac agttatcaca
      661 gtaaagggtg agacaatggg caaaggtggt ggcaaggcgc acacgccggt tgaggcaaag
      721 gacaatctta agtccacgca gatgatgagc gtgattgatg cgattggtga agggccgatt
      781 gaaggt
//
LOCUS       DAZUFB010000407          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZUFB010000407 DAZUFB010000000
VERSION     DAZUFB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.418"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..69)
                     /locus_tag="ACHY3M_005557"
     CDS             complement(<1..69)
                     /locus_tag="ACHY3M_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320213.1"
                     /translation="MQQLSFLPGEMTPGERSLILRAL"
     gene            complement(85..570)
                     /locus_tag="ACHY3M_005558"
     CDS             complement(85..570)
                     /locus_tag="ACHY3M_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4320214.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(661..>785)
                     /locus_tag="ACHY3M_005559"
     CDS             complement(661..>785)
                     /locus_tag="ACHY3M_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFS4320215.1"
                     /translation="SGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          185 a          202 c          225 g          173 t
ORIGIN      
        1 cagggcccgc agaatgagac tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag
       61 ctgctgcatt gtccctgcct ccattcagtc aatgatgcgc ataatggcgc tgcattccgg
      121 atgctgcagg gcgtaatccc gcagccggta atagtggacc gtcatggcat aacactccgt
      181 acgacaggca tgatgactgt acgtcatcag acaggcagca ataccggcgg cttccgggct
      241 catttcagcg cggttgccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc
      301 atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc
      361 accgcagtag tcttcgcaaa gacggtccat ccagccgaag acacggggct ccagggtcac
      421 ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt catcagggac
      481 gggagtggca accagctgag gtgggttatt ccctgatgca gtggttacgg aaacagaagg
      541 tgtggtggaa ttatgcgaaa cggttgtcat gaggttattc cttataaaaa gtaaatgaat
      601 ggaagaagcc ccggggcagg aacagatgtg agtcaggact gcactttcag tgaaacggca
      661 tcagcgcatg ctctccagca gcgtttctgc catcacccac aatgcgcgat tgagcttaat
      721 gtcggtatca atgctgtgaa tggcccgggt atggatacgt tttccttttg cactgcgacc
      781 ggaaa
//
LOCUS       DAZUFB010000408          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZUFB010000408 DAZUFB010000000
VERSION     DAZUFB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.419"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..785
                     /locus_tag="ACHY3M_005560"
                     /pseudo
     CDS             1..785
                     /locus_tag="ACHY3M_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          132 a          241 c          276 g          136 t
ORIGIN      
        1 tacgcgatga tgcactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
       61 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgtggcggta
      121 aggcagcgca gccggacggc cacacgctgg cacggctgtg ggcgtcgctg ccaccggata
      181 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
      241 tggggtggtc tgagcgggtg ccgggtgctg aggacttact gccagcgccg ctgccgccgt
      301 accgggtgct taccgggatg gcggaccgct tcgggcggac gctggcgtac cggcgtgagg
      361 ccgccggtga cctggccggg gaaatcaccg gcgtgaggcc gccggtgacc tggccgggga
      421 aatcaccggc gtgacggacg gtgccgggcg ggagttccgt ctggtactga ccacacaggc
      481 acagcgggcg gaagaggccc gtaaaccgca caccgcttcg ttatcttccc ctgacagtcc
      541 ccgccctctc tcagctcctt ccttccccga cacactgccc ggtacggaat acggcgctga
      601 cagcggtatc cgtctttcgg cggtgtggct gatgcacgac ccggaatacc cggataattt
      661 acctgccgcg ccactggtgt gctacgactg gacgccacgc ggtgagctgg cggcggtgta
      721 tgaccgcagc ggtacgcaga tgcgccactt tacttacgat gataaatacc ggggccggat
      781 ggtgg
//
LOCUS       DAZUFB010000409          782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZUFB010000409 DAZUFB010000000
VERSION     DAZUFB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.420"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>782
                     /locus_tag="ACHY3M_005561"
     CDS             <1..>782
                     /locus_tag="ACHY3M_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4320216.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASGVTK
                     TLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAAT"
BASE COUNT          158 a          191 c          280 g          153 t
ORIGIN      
        1 cagcggacag gcacggcatt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgccggtgat gtactgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggct cggtgatcac ccagttcagt
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgtgc ccgcgttaat cagccagctg
      361 gggacattgc gtgatgaata tgccgccatg ccagaaacgg tctctgacgg gatcacaaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcatgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggtggcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cacggcgcgg gcaagagcag ccgtgtaccg tgctcagcag
      721 gccgtggcgg cagcccgcgg gacggagatg cagattgctg cagaggcccg tctggcggcc
      781 ac
//
LOCUS       DAZUFB010000410          780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZUFB010000410 DAZUFB010000000
VERSION     DAZUFB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.421"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>780
                     /locus_tag="ACHY3M_005562"
     CDS             <1..>780
                     /locus_tag="ACHY3M_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFS4320217.1"
                     /translation="YQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALF
                     TAMLAQFRADLSVFNAQCHGGSAADVPWICGDTTYYWKNTYGTQYNTIYGAYKNRESE
                     GVYFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARR
                     SIIPDRLATAILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSADTSVRTISLLPAAGE
                     AAAQGWSIKDGGIQLSDGVFKITKQSNKTWSLTHPVDDAIT"
BASE COUNT          192 a          212 c          217 g          159 t
ORIGIN      
        1 tatatcagga cctgattgcg cgcactaaag ctgcattaca gaagaacccg aaaaatgtgt
       61 tgctggcggt gtgctggatg cagggagagt ttgacatgag cgccgccacc cacgcacagc
      121 aacctgcgct gtttacagcc atgctggcac agtttcgtgc tgacctctcc gtgtttaacg
      181 cgcagtgcca tggtggcagt gctgcagatg tgccgtggat ttgtggtgac acgacgtatt
      241 actggaaaaa tacctacggc acccagtaca acaccattta cggggcgtac aaaaacaggg
      301 agagtgaggg cgtttatttt gtgcccttca tgacagacgg taacggcgtc aataccgcca
      361 ctaacgcgcc ggcagaagat ccggatattc cggcatcagg atattacggt gcggcatcga
      421 gaacgaatgg aaaccaggta tcatcaaacc gcccgacaca tttcagttca tgggcgcgca
      481 ggagcattat tccggatcgt ctggcaaccg ctattctgaa cgcagccggg cgcacctcag
      541 ccttcatcag tggtaaggca ccggaaatca aaccctcgcc cggcggcaac acgccatcgg
      601 gtccgtctgc agatacgtcc gttcgcacaa tctccctgct gccggcagcc ggagaggctg
      661 ctgcgcaggg ctggagcatt aaggatggcg gaattcagtt gtcagatggt gtatttaaga
      721 tcaccaagca gagcaataaa acctggtccc tgacgcatcc ggtggatgac gcaattaccc
//
LOCUS       DAZUFB010000411          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZUFB010000411 DAZUFB010000000
VERSION     DAZUFB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.422"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(189..731)
                     /gene="fimA"
                     /locus_tag="ACHY3M_005563"
     CDS             complement(189..731)
                     /gene="fimA"
                     /locus_tag="ACHY3M_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4320218.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          231 a          155 c          153 g          214 t
ORIGIN      
        1 aaaataagaa atataaagaa atgattgctt tggtttctta caacacatca ttcccttttc
       61 atttaataaa gcggcatgat acaaagtgaa catgatttcg atttacattg ccaccatacc
      121 gcttcagaca accggtacat tactttgtta taagacgtga tatataattc aaaacaacgt
      181 ggttttgatt attcgtatct cataataaaa gtcgcgtcag catttgcttg ccctgctgat
      241 gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc
      301 aagttctggt tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca
      361 agtatttcaa taccgacacc cgttgctgtt gttgtattgg tactggatgc aatggccaat
      421 aaattatcat tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca
      481 acaggatcgc agtcgttcaa ctgaatggtg aatggaatta atgcagaggt attaccaaca
      541 gcattgaaaa tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta
      601 ttcactgaac aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca
      661 acggcagcat aactacccgt agcggcgaat agtgcggcag ccagcgcaga cgaaataaat
      721 cttaatttca tatatattcc ttcaatctca ttt
//
LOCUS       DAZUFB010000412          752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZUFB010000412 DAZUFB010000000
VERSION     DAZUFB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.423"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>752
                     /locus_tag="ACHY3M_005564"
     CDS             <1..>752
                     /locus_tag="ACHY3M_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320219.1"
                     /translation="AELQKNAIIPSYKEHMAILQKQLRISRHAQEQLAKLSTQALISD
                     SILKQFSHRPNLAEIGNEYRKHIDILQKQALAFFPRDANKYIQMLRKQAEIAMPSRNQ
                     FEMLTKQAGLNNMQAMLNELRQNVSHKIDLNNEITESLKQYTLAQKASLHQIMEQSLS
                     SIAQAYVEGAIETSHNESDVQDKELNKSSSTFIDSFKSLPHPLQFIIMWLLTEVVLGA
                     IADYAKEQILSQIHKTESYSVSLYEDAPISKQ"
BASE COUNT          277 a          143 c          130 g          202 t
ORIGIN      
        1 tggcagaact gcaaaaaaac gcaatcattc catcgtataa agaacatatg gcaatcttgc
       61 aaaaacagtt aagaatatcg cgccatgctc aagaacaact tgcaaagttg agtacacaag
      121 ccttaattag tgattctatt ctaaaacagt tttcgcatag accgaatttg gcagaaatag
      181 gcaacgagta tcgtaaacat attgatattt tacagaaaca ggctctggct tttttcccgc
      241 gtgatgccaa taaatacatt caaatgctaa ggaaacaggc agaaattgct atgccgtcac
      301 gaaaccagtt cgagatgctt actaagcaag ctggtttaaa taacatgcaa gcaatgctta
      361 atgaattgcg acaaaatgta agtcataaga tagatttaaa taatgaaata acagaatcat
      421 taaaacaata tacccttgcg caaaaggctt cattacatca aataatggag caaagtcttt
      481 catcaattgc acaggcttat gtcgaaggtg ctattgaaac atctcacaac gaaagtgatg
      541 ttcaagacaa agaattaaat aaatccagtt cgacgttcat tgattcattt aagtcgctcc
      601 ctcacccgct tcagtttatt attatgtggc ttttgacaga agtggtgctt ggcgctattg
      661 ctgattatgc aaaagaacaa atcttatcgc aaatacacaa gacagaatca tattctgtat
      721 ccctatatga ggatgcacca atatcaaaac aa
//
LOCUS       DAZUFB010000413          742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZUFB010000413 DAZUFB010000000
VERSION     DAZUFB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.424"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..121)
                     /gene="ydcD"
                     /locus_tag="ACHY3M_005565"
     CDS             complement(<1..121)
                     /gene="ydcD"
                     /locus_tag="ACHY3M_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFS4320220.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKT"
     gene            complement(105..>742)
                     /locus_tag="ACHY3M_005566"
     CDS             complement(105..>742)
                     /locus_tag="ACHY3M_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS4320221.1"
                     /translation="HQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGW
                     NMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDA
                     IVGGAHNGAADAMRHCYLMCRMTKTFGSIIADVIGKNHEAAGDRQGQPAKERIMDLKN
                     NTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          209 a          185 c          130 g          218 t
ORIGIN      
        1 gtgttttatg tccataaaat acgatgtcag aatttgaatt gttatcaaac ccaaggtaaa
       61 caacaaataa acaaagggta attaaaaaaa tgacaagttt gcctctaata atttgaagca
      121 tctgaactcc cttgctttgc ttttattgga ttatctgctt ttataccatc taacccgaag
      181 agttgcccag tgttatattt ttcaatacat gcatcgctac atttggcaga aaaatcgcca
      241 caagcaatac cgacagtgtt atttttaaga tccatgattc tttctttagc tggctgacct
      301 tgtctatccc ctgccgcctc atgattttta cctatcacgt cagctattat tgatccaaat
      361 gtcttagtca ttcgacacat caagtaacaa tgtctcattg catcggcggc cccattatga
      421 gctccgccaa caatagcatc catatcataa ccgaactggt aagcactgtt agtaatgctg
      481 tttgcagcaa tcaatccagg tacaccagat acggcataaa ttagtccacc tgatgtcata
      541 ttctcaatcg catctaaccc cattgggtct atcacttgta tgggattcaa cggatactga
      601 tacatattcc atccacctct caacccaatc gggtccgggg tgatataccg cccctgcaac
      661 ggatcgtagt accggtgacg gttatagtac agccccgact ccttatcata ctgctgcccc
      721 ggcagccggt acggctggtg ca
//
LOCUS       DAZUFB010000414          738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZUFB010000414 DAZUFB010000000
VERSION     DAZUFB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.425"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>738
                     /locus_tag="ACHY3M_005567"
     CDS             <1..>738
                     /locus_tag="ACHY3M_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS4320222.1"
                     /translation="RDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQP
                     DGHTLARLWGALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRE
                     LTGLADRFGRTLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSS
                     SDSSRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAG
                     ELLAVYDRSNTQVRAFTYDAQHPGRMV"
BASE COUNT          120 a          230 c          266 g          122 t
ORIGIN      
        1 tacgtgatga cggactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
       61 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgcggtggta
      121 aggcagcaca gccggacggc catacgctgg cgcggctgtg gggggcgctg ccgccggata
      181 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
      241 tcggctggtc agagcgggtg ccgggtgctg aggacgtgct gccagcgccg ctgccgccgt
      301 accgggagct gacggggctg gcggaccgct tcgggcggac gctgacgtac cggcgtgagg
      361 ccgccggtga cctgaccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc
      421 gtctggtgct gaccacgcag gcgcagcgtg cggaagaggc ccgcacctct tcgctatctt
      481 cttctgacag ttcccgccct ctctcagcct cagcgttccc cgacacactg cccggtaccg
      541 aatacggccc cgacaggggt atccgccttt cggcggtgtg gctgatgcac gacccggcat
      601 acccggagag cctgcccgct gcgccactgg tgcggtacac atatacggaa gccggtgaac
      661 tgctggcggt atatgaccgc agcaatacgc aggtgcgcgc tttcacgtat gacgcgcagc
      721 acccgggccg gatggtgg
//
LOCUS       DAZUFB010000415          732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZUFB010000415 DAZUFB010000000
VERSION     DAZUFB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.426"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..>732)
                     /locus_tag="ACHY3M_005568"
     CDS             complement(32..>732)
                     /locus_tag="ACHY3M_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFS4320223.1"
                     /translation="PFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHST
                     IYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEH
                     RQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGD
                     PLGEMRLVSRVFEM"
BASE COUNT          163 a          198 c          190 g          181 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gt
//
LOCUS       DAZUFB010000416          727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZUFB010000416 DAZUFB010000000
VERSION     DAZUFB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.427"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..592
                     /locus_tag="ACHY3M_005569"
                     /pseudo
     CDS             1..592
                     /locus_tag="ACHY3M_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT          202 a          171 c          204 g          150 t
ORIGIN      
        1 gaatcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgcat tccgctaccc
       61 gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac ttggctggat
      121 gcgctaccgg aatagccgtc aggtcacggg agttgtgaaa aatgtcactg tcagccagtc
      181 ctgcggtaag tggtacatca gtattcagac agaaagtgaa gtatccactc cggttcaccc
      241 ttcagcatca atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
      301 cacagtcttt gagcctgtaa acagttttca gaaaaaccag aagacgctgg cgagacttca
      361 gcgacagtta agccgcaggg tcaaattcag caacaactgg cagaagcaga aacgcaaaat
      421 acagcgactg cattcccgta tcgcaaatat ccgcagggac taccttcata aagtcacaac
      481 gaccgtcagc aaaaaccacg caatgatagt cattgaggat ttgaaggtca gcaacatgtc
      541 aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa agcagccccg
      601 gttgggtagt tatgtgggat ggctggttca gacatcagga tatggctgag ggttacctgg
      661 tgatgaagtc tcttgatcag gagatctgat caagagacag gtcaaagatg ggggacaacg
      721 tctggtg
//
LOCUS       DAZUFB010000417          716 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZUFB010000417 DAZUFB010000000
VERSION     DAZUFB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.428"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..332
                     /locus_tag="ACHY3M_005570"
     CDS             <1..332
                     /locus_tag="ACHY3M_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320224.1"
                     /translation="PSFVVSGDAGKITISENGKVTPPSHQHSEVLIEFAIDYLKNNKK
                     QGLMKCIGRCMGYLQIAAEIEALASGADKDAVVREALLREFDNPPFKKVPAYWFHPGL
                     TYLKGRI"
     gene            375..>716
                     /locus_tag="ACHY3M_005571"
     CDS             375..>716
                     /locus_tag="ACHY3M_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFS4320225.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIG"
BASE COUNT          187 a          162 c          197 g          170 t
ORIGIN      
        1 ggccatcctt tgttgtatcc ggtgatgctg ggaaaataac catctcagaa aatggaaaag
       61 taacaccgcc atcgcaccag catagtgagg tgctcattga atttgccatt gattacctga
      121 agaacaataa aaagcagggg ctgatgaagt gcattggtcg ttgcatggga tatctgcaga
      181 tagctgctga gattgaagcg ctggccagtg gtgcggacaa ggatgcagtt gtgcgggagg
      241 ctcttcttcg tgagtttgac aacccgccct ttaaaaaagt gccggcttac tggtttcatc
      301 caggactgac ttatcttaaa ggacgtatat aagctggctc gttatctgtt gccgataaat
      361 cctgataaat atccatgaac accaaaatca aatacggcct gtcggctgcc gttctggcgc
      421 tgattgccgc tggtgcgcct gcgcctgaca ttctcgacca gtttctggat gaaaaggaag
      481 gtaaccacac cacggcatac cgtgatggtg cgggtatctg gaccatctgc cgaggtgcca
      541 tcatggtgga tggtaagcct gtgattcctg gcatgaagct gtcgaaggaa aaatgcgacc
      601 aggttaacgc cattgaacgt gataaggcgc tggcatgggt ggagaaaaac atcagagtgc
      661 cactgaccga accccagaaa gcggggattg cgtcattctg tccgtacaac attggc
//
LOCUS       DAZUFB010000418          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZUFB010000418 DAZUFB010000000
VERSION     DAZUFB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.429"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..259
                     /gene="rrrD"
                     /locus_tag="ACHY3M_005572"
     CDS             <1..259
                     /gene="rrrD"
                     /locus_tag="ACHY3M_005572"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFS4320226.1"
                     /translation="TVARQINPYINVDIPETTRGALYSFVYNVGAGNFRTSTLLRKIN
                     QGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            476..661
                     /locus_tag="ACHY3M_005573"
     CDS             476..661
                     /locus_tag="ACHY3M_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4320227.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWVMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          186 a          185 c          191 g          150 t
ORIGIN      
        1 cacggtcgcc agacaaatta acccgtacat caacgtcgat ataccggaaa caacgcgcgg
       61 cgctctttac tcgttcgttt acaacgtggg cgctggcaat ttcagaacat cgacgcttct
      121 tcgcaaaata aaccagggcg atatcaaagg cgcatgtgat cagctacggc gctggacata
      181 cgctggcggt aagcaatgga aagggctgat gactcgccgc gagattgagc gtgaagtctg
      241 tttgtggggg caacaatgag cagggtaacc gttattatct ccgctctggt tatctgcatt
      301 atcgcctgcc tgtcatgggc tgttaatcat taccgcgata acgccattac ctacaaagcg
      361 caacgcgata aaaaagccag tgagctgaag ctggcgaacg tgacaattac tgatatgcag
      421 gtacgccagc gtgatgtcgc tgcacttgat gccagatact cgagggaatt agccgatgcg
      481 agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg cctgcgcatc
      541 aacgccaact gtccaggctc cttgcgtaaa gcccccatca cctccggcgt gggtaatgca
      601 accggtcccc gactggcaga agccgctgaa cgggattatt tcatcctcag agaacggctg
      661 atggcaatgc agaagcaact ggaaggagca caggaatata tccgtaccca gt
//
LOCUS       DAZUFB010000419          699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZUFB010000419 DAZUFB010000000
VERSION     DAZUFB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.430"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(74..>699)
                     /locus_tag="ACHY3M_005574"
     CDS             complement(74..>699)
                     /locus_tag="ACHY3M_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320228.1"
                     /translation="SGVAALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSKNFEDGHI
                     STGTIFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLELFNASQSKALP
                     FAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSN
                     MDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          238 a          135 c          114 g          212 t
ORIGIN      
        1 aacaacatca ttgtgatgat aaatataaaa taggcatggc gaaaaaaaaa tcaccacgcc
       61 taaaatataa taattatggt agcatcattg atacataatc cacaccaatc cttgagctat
      121 actgagacgc tatagcctga tatctttctg cataaccagt tctcagttga gatttaagtt
      181 tgagtcggac aggaacattt tgcacgttgc catccatatt acttaaaaac acggcagaaa
      241 taatattttt ttcttcgcca tcaactgttg ttccatgatt caacaccacc atataatcaa
      301 caacaggaag cgttttatcc ccttcgaaaa tagagagata ttttctttga tttttatgca
      361 ttacatatat atatttcgaa tgttcagcaa atggcaatgc tttactctga ctggcgttaa
      421 aaagctccag aactttaatg agcctgtgcg gacttaatct tacatggtga gggtcgttac
      481 cctgagtagg aaccaaatca catgccgcag atacacataa ataccatttg ttcgactctg
      541 tatcaaagaa aatagtgcca gtagaaatat gaccatcttc aaaattcttt gaagacaaat
      601 tcatatttaa agcatgatac atttcgtgat aagtatcatt atttgatggc agatccattt
      661 ttgaagagca atattggagc aatgcagcaa ctccgctgt
//
LOCUS       DAZUFB010000420          695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZUFB010000420 DAZUFB010000000
VERSION     DAZUFB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.431"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..676
                     /locus_tag="ACHY3M_005575"
     CDS             134..676
                     /locus_tag="ACHY3M_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4320229.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT          209 a          126 c          151 g          209 t
ORIGIN      
        1 atctataaat taaagatttg tcacttattg gatttagtat gcttgattta aatggcggtc
       61 aattattgtt attattaagt ctaatgtcat ttaaaggcat tatcatattt acgaagagat
      121 caggatgatg acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg tatgtgcaac
      181 gtccagcgtt atggcagctg atgataatgc tatcacggat ggcaaagtaa catttaatgg
      241 taaagttatt gctccagctt gtaccctggt agctgcgacg aaagattcag tagtcacatt
      301 gccaaatgtc agcgccacca agttgcaaac taatggagca gtctctggcg ttaaaactga
      361 tgtaccaatt gccttagaag gctgtgatgt tactgtaact aaaaatgcga cgtttacctt
      421 tagtggtacg gctgacggcg tgcaacctac agcatttgcc aaccaggcta ccaccgatgc
      481 agcgacgaat gttgctctac aaatgtatct gccagatggt tctacatcgg taactcccgg
      541 taccgaaacg tcgaatattc agttagcgga ttctgctgag caaacagtga cctttaaagt
      601 tgattatatc gctacgggga aagcgacttc tggtaatgtg aatgcggtaa caaatttcca
      661 tattaactat tattaataga actcattaat tgttt
//
LOCUS       DAZUFB010000421          692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZUFB010000421 DAZUFB010000000
VERSION     DAZUFB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.432"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..245
                     /locus_tag="ACHY3M_005576"
     CDS             <1..245
                     /locus_tag="ACHY3M_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4320230.1"
                     /translation="VNTGALLTMSVSVNGGTPPYKYAWKKDGQPVEGQTTDTFSKANA
                     QSGDKGAYTCMVTDSAEQPQSITSDACTVTVNGAGG"
     gene            251..625
                     /locus_tag="ACHY3M_005577"
     CDS             251..625
                     /locus_tag="ACHY3M_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS4320231.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
BASE COUNT          155 a          163 c          229 g          145 t
ORIGIN      
        1 ccgtgaatac aggtgcgctg ctgacaatgt cagtcagtgt caacggggga acgccgcctt
       61 ataaatacgc ctggaagaag gatggtcagc cggtcgaggg acagactact gacactttca
      121 gtaaagccaa tgcgcagtca ggtgataagg gggcttatac ctgcatggta acggattctg
      181 cagaacagcc gcagagcatt acctctgatg cgtgtacggt aacggttaat ggtgcgggcg
      241 gataaggctt atggcaaaag atctgaaaac actggcgctg gccagactgt cggggttccg
      301 tcataaaacg gtgaaggtgc cggaatggag aaatgtcagc gtggtgctgc gggagccttc
      361 ggcagaggcc tggtatctgt ggcaggaagt gctcaatggt gatggagagg atgacgatac
      421 cctgtcggtg gtggcgaaaa cccgccgtaa cctggaagcg gatgtgacgc tgttctgcga
      481 tgtcctgtgt gatacggatc tgcagcgggt gttcgctccg gacgaccgtg agcaggtgct
      541 ggccgtctat ggtccggtac atgcccggtt gctgcgtcag gcactggaac tgatcgctga
      601 tgcagagtcg gccagaaaaa agtagcccgc ccggaaattc gctttctgat gcgacttgcg
      661 ctccgtctgg ggcgcacctt atccgaactg cg
//
LOCUS       DAZUFB010000422          691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZUFB010000422 DAZUFB010000000
VERSION     DAZUFB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.433"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(179..271)
                     /locus_tag="ACHY3M_005578"
                     /pseudo
     CDS             complement(179..271)
                     /locus_tag="ACHY3M_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
     gene            313..>691
                     /locus_tag="ACHY3M_005579"
     CDS             313..>691
                     /locus_tag="ACHY3M_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4320232.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPD
                     PTLPEVMAGQTISSDCDPPRGGII"
BASE COUNT          157 a          146 c          208 g          180 t
ORIGIN      
        1 gatataaagg gacaggagaa cagaatacgt ccggaaacgc gcggtcaaaa aaatgctaaa
       61 aaatatctga gagagttatt gcctgttacc ataagaaaaa gcgactttag tggtcgcttt
      121 ttgtgtcata tataagtcgt ttaagtaaac ctgtctgaac aggtgctctg gtcgtgttct
      181 ggtcaagagc gatgttaatt tgttcaatca tctggtttgg aaatcggatg ttgcgggttg
      241 ttgttctgcg ggtccggttt ttcggtgaca ttttctttcc tctggtgaca agctatatgg
      301 cgaggatttt acatggccgt gcttcgtacg ttaccgggca gaatcaaaac tctgaatacc
      361 cggcgggtga atgtcctgaa gggtgaacag cgtcgggtca gtggcagtgc ccgggtttcc
      421 ctcaagcgtc gtatctggct gagggatgcc ggacagtgct gtctctgtgg gcgtgtggtt
      481 gacctttgtg acagtgaact cgatcaccgc attgcacttc agttcggtgg tggtaatgag
      541 gagacgaatc tctggacgct ctgtaccgaa tgccatcgcc agaagtcagt cagtgaaacg
      601 gcgagtggta tgccggaccc gacgctgccg gaggtcatgg cagggcagac gatatcatcg
      661 gactgtgacc cgccccgggg ggggatcatc c
//
LOCUS       DAZUFB010000423          685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZUFB010000423 DAZUFB010000000
VERSION     DAZUFB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.434"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>685
                     /locus_tag="ACHY3M_005580"
     rRNA            <1..>685
                     /locus_tag="ACHY3M_005580"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          184 a          144 c          220 g          137 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg
      601 ctgatgcgtt gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggcat
      661 gctggaggta tcagaagtgc gaatg
//
LOCUS       DAZUFB010000424          661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZUFB010000424 DAZUFB010000000
VERSION     DAZUFB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.435"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..>661)
                     /locus_tag="ACHY3M_005581"
     CDS             complement(52..>661)
                     /locus_tag="ACHY3M_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4320233.1"
                     /translation="QGGERYFFCNELNEKGEPVTWQGREYQAYPIEGSGFEMNGKGSS
                     ARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELT
                     DRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVA
                     DEFDKPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          138 a          235 c          155 g          133 t
ORIGIN      
        1 cacaccgccg ggcatgcgcc agaatcgctg attctgtctg tgtcattgga tttactgcga
       61 aagtttgtta atggaaagga aaccgccaaa attagccacc atgccgcgca tctcacaccc
      121 gcgcatgcac ttgctgcatc tgtccttacg gatatcggtg gtgggtttat cgaactcatc
      181 cgccaccgca ggaccgttat acccgcattc atctccccgg taatcccaca tacaggtgtt
      241 cgccagcatg atgcgaccgg gaaacagcgc tccgtccgtc tccgtcggtg tggccagcac
      301 aaacgaggcc gtcatggccg tcagctctga catctgctcc accacccacc ggtcagtcag
      361 ctcctgctcc gggtcggcct ccggattgcc tgccacaaag ttcaccgcat ccagaaaacg
      421 cgcatacacc cggcggcgga ccaccgtggc acccaccagg ctctgcaaat cctccgccat
      481 cccggtgaca agaccgaaca gattggacac cgtcagcgac gggcgggcac tgctgccctt
      541 tccgttcatc tcaaagccgc tgccctcaat cgggtacgcc tgatattcac gcccctgcca
      601 ggtcaccggc tccccttttt cattcagctc attgcagaaa aaataccgct caccgccctg
      661 c
//
LOCUS       DAZUFB010000425          661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZUFB010000425 DAZUFB010000000
VERSION     DAZUFB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.436"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..555)
                     /locus_tag="ACHY3M_005582"
     CDS             complement(<1..555)
                     /locus_tag="ACHY3M_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4320234.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDTLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPIESVAIDIAYEGSGSGD"
BASE COUNT          150 a          198 c          165 g          148 t
ORIGIN      
        1 gtcgccactg ccggagcctt cataagcaat atcaatggcc acggattcga tcgggttaaa
       61 ctgcacgcca gctccccacg ccagagacgt gttgctgtgg cgaccgtcat cacttccggt
      121 cagcacatcg tgcgttttcc ccttgttgtc agttacgcgg agataatccc cggagaaagt
      181 cgacacacgg ctgtaagcca cgcccgccat cgcatacgcg ctgaaccatt cattcacgcg
      241 cacagagggc cccgccatca cgctgaacca gcggttacgc acggaatctt catgccagcg
      301 ggtatcgctg taacgggtaa gctggcgatt cctgtctcct gcatagctga atgacgtcac
      361 cagccccagt gtgtccgtaa actcataacg gtatttcacg ttaatcccgt taagagtatc
      421 gctaccggga gcgctcgtcc gggcatgaag ataccccgcg ctcagcgtgg cctgctgctc
      481 agacgcccat gcaggcgcgc cggatacggc cagacagatg gctgcggaca aaatggcggc
      541 ataaagttta cgcataatta cctctcgctt ttctgcaata aaaaaaggcg tcatttctga
      601 cgcccgttct gggttataaa attcagctga tactgatacc tgctgtggat tttttcatca
      661 c
//
LOCUS       DAZUFB010000426          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZUFB010000426 DAZUFB010000000
VERSION     DAZUFB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.437"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..584)
                     /locus_tag="ACHY3M_005583"
     CDS             complement(<1..584)
                     /locus_tag="ACHY3M_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4320235.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFD"
     gene            complement(584..>658)
                     /locus_tag="ACHY3M_005584"
     CDS             complement(584..>658)
                     /locus_tag="ACHY3M_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4320236.1"
                     /translation="CREWSVTDNARYSDFSCTIEQVVN"
BASE COUNT          138 a          242 c          144 g          134 t
ORIGIN      
        1 ttatcgaact catccgccac agccccgccc gtgtaaccac actcatcaga gcggtaggtc
       61 cacatacagg tattcgccag cataatgcga ccgggaaaca gcgctccgtc cgtctccgtc
      121 ggtgtcgcca gcacaaacga ggccgtcatg gccgtcagct ctgacatctg ctccaccacc
      181 cagcggtcgc tcagctcctg ctccgggtcc gcttccggat tgcctgccac aaaattcacc
      241 gcatccagaa agcgggcata cacccggcgg cggaccaccg tggcccccac caggctctgc
      301 aggtcctccg ccatcccggt gaccagacca aacagattgg acaccgtcag cgacggtctg
      361 gcactgctgc ccttcccgtt catctcaaaa ccgctgccgt caatcgggta tacctgatat
      421 tgccgcccct gccaggtgac cggctcccct ttttcattca gctcattaca gaaaaaatac
      481 cgctcaccgc cctgcaccgt caggtcaatt tcccagagca ccacccgcgg tgactgctct
      541 gatttaaccg actcgttcag gctttcttcg tgaatatcct gcatcagttc accacctgct
      601 ctatcgtgca actgaaatca ctgtaccggg cattatccgt gacactccac tcacggca
//
LOCUS       DAZUFB010000427          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZUFB010000427 DAZUFB010000000
VERSION     DAZUFB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.438"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..584)
                     /locus_tag="ACHY3M_005585"
     CDS             complement(<1..584)
                     /locus_tag="ACHY3M_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4320237.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFD"
     gene            complement(584..>658)
                     /locus_tag="ACHY3M_005586"
     CDS             complement(584..>658)
                     /locus_tag="ACHY3M_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4320238.1"
                     /translation="CREWSVTDNARYSDFSCTIEQVVN"
BASE COUNT          139 a          241 c          148 g          130 t
ORIGIN      
        1 ttgtcgaact catccgccac agccccgccc gtgtaaccac actcatcaga gcggtaggtc
       61 cacatacagg tattcgccag catgatgcga ccgggaaaca gcgccccatc cgtctcggtc
      121 ggtgtcgcca gcacaaacga ggccgtcatg gctgtcagct gcgacatctg ctccaccacc
      181 cagcggtcac tcagctcctg ctccgggtcc gcctccggat tgcccgcaac gaaattcacc
      241 gcatccagaa aacgggcata cacccggcgg cggaccaccg tggcccccac cagactctgc
      301 aggtcctccg ccatcccggt gacaaggcca aacagattgg acaccgtcag tgacgggcgg
      361 gcactgctgc ccttcccgtt catctcaaaa ccgctgccgt caatcgggta tgcctgatat
      421 tgccgcccct gccaggtgac cgcctcccct ttttcattca gctcattaca gaaaaaatac
      481 cgctcaccac cctgcaccgt caggtcgatt tcccagagta ccacccgcgg tgactgctct
      541 gatttaaccg actcgttcag actttcttca tgaatgtcct gcatcagttc accacctgct
      601 caatcgtaca actgaaatca ctgtaccggg cgttatccgt gacgctccac tcccggca
//
LOCUS       DAZUFB010000428          652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZUFB010000428 DAZUFB010000000
VERSION     DAZUFB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.439"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..605
                     /locus_tag="ACHY3M_005587"
     CDS             <1..605
                     /locus_tag="ACHY3M_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFS4320239.1"
                     /translation="NEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQD
                     PIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKALTK
                     WWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDGKKT
                     DRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
BASE COUNT          213 a          135 c          154 g          150 t
ORIGIN      
        1 tgaatgaaga gaacccgcat cagctgcagc agcttatccg cctgccgggg cagcagtatg
       61 atgaggagtc cggcctgtat tacaaccgcc accgctatta tgacccgctg caggggcggt
      121 atatcactca ggatccgatt gggctgaagg ggggatggaa tttttatcag tatccgttga
      181 atccagttac gaatacagat cctctggggt tagaagtttt tcctagacca ttccccttgc
      241 caattccatg gcccaaaagc cctgcacagc agcaagcaga tgataatgct gcaaaagcat
      301 tgacaaaatg gtggaacgat acagcatcac aaagaatatt tgactctcta atattgaata
      361 atccgggact agcattagat ataacaatga tagcttctcg tggaaatgtt gcagacacag
      421 ggataactga tcgtgtcaat gacataataa atgacagatt ctggagtgat gggaaaaaaa
      481 ccgacagatg tgacgtactt caggaactaa ttgattgtgg tgatattagt gctaaagatg
      541 caaaaagcac acagaaagcc tggaattgtc gtcactccag acagtcaaac gataaaaaaa
      601 gatagccctt gtggaggttc ctgcaatgtc aaatacatac cagaaaagaa ag
//
LOCUS       DAZUFB010000429          651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZUFB010000429 DAZUFB010000000
VERSION     DAZUFB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.440"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>651
                     /locus_tag="ACHY3M_005588"
     CDS             <1..>651
                     /locus_tag="ACHY3M_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4320240.1"
                     /translation="VDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSI
                     RENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQY
                     LAQAKWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLAPGSYRLTVR
                     AVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPKLAVYDPTVQFEFWFSE"
BASE COUNT          129 a          188 c          218 g          116 t
ORIGIN      
        1 gtggacatca ccgcacaccc cgcgccggac cggatacagg tcagtaccct gccggatggc
       61 gtggagacat acggtgtgtg gggactctcc ctgccgtcac tgcgtcgtcg cctgttccgc
      121 tgtgtttcca tccgggaaaa cacggacggt acctttgcca tcacggcggt gcagcacgta
      181 ccggaaaagg aagccatcgt ggataacggg gccagctttg agccgcagtc aggcaccctg
      241 aacagcgtca tccctccggc agtgcagcac ctgacggtgg aggtgagcgc agctgacggt
      301 cagtatctgg cacaggcgaa atgggacacg ccgaaggtgg tgaagggcgt gagctttatg
      361 cttcgcctga ccgtggccgc ggatgacggc agtgagcggc tggtcagcac ggcccggacg
      421 acggaaacca cataccgctt cacgcaactg gcgccgggga gctacaggct gacagtccgg
      481 gcggtaaatg cgtgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgccgca
      541 ccggcagcgc cgtcacagat tgagctgaca ccgggctatt ttcagataac ggcggtcccg
      601 aaactggctg tatatgaccc gacggtacag tttgaattct ggttttcgga g
//
LOCUS       DAZUFB010000430          647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZUFB010000430 DAZUFB010000000
VERSION     DAZUFB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.441"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..528
                     /gene="ygeG"
                     /locus_tag="ACHY3M_005589"
     CDS             58..528
                     /gene="ygeG"
                     /locus_tag="ACHY3M_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4320241.1"
                     /translation="MFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAFDYH
                     EKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPYDDY
                     SVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          241 a          104 c          107 g          195 t
ORIGIN      
        1 aatttggcat cacgagacac atcacagagg aatattatga gcacagaaac aatttaaata
       61 ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg cattatcgaa aggagaaaat
      121 ctggcattac tacatggttt aactcctgat atccttgata gaatatatgc atatgcattc
      181 gactaccatg aaaagggtaa tataacggac gcagaaattt attataaatt tttgtgcatt
      241 tatgcgttcg aaaatcatga gtatctaaaa gattttgcat cagtatgtca gcccaaaaag
      301 aaatatcaac aagcatatga cctttacaaa ctaagttaca attactcccc gtatgatgac
      361 tattcagtta tttatcgtat gggtcaatgt cagattgggg ctaaaaatat cgataacgca
      421 atgcaatgtt tctatcacat tattaacaat tgtgaggatg atagtgttaa gagtaaagcg
      481 caggcatata ttgaactctt aaacgataat tcagaagata atggctaagc attacaatca
      541 tttggttaaa tttgctggtt atagtgcagt taaccactat aaccagcacc tcttatgttt
      601 tcttaaatca gcatcttaaa acagttaaga gcatactata aatcatt
//
LOCUS       DAZUFB010000431          647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZUFB010000431 DAZUFB010000000
VERSION     DAZUFB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.442"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..546
                     /locus_tag="ACHY3M_005590"
     CDS             <1..546
                     /locus_tag="ACHY3M_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFS4320242.1"
                     /translation="AFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPG
                     DEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTG
                     QTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMES
                     PSKDATPGNVYAQADYKIAYE"
BASE COUNT          204 a          136 c          142 g          165 t
ORIGIN      
        1 gcttttaaaa agtcacttct tgttgctggc gtagcaatga ttctttccaa taacgtattt
       61 gctgacgaag gtcacggcat tgttaaattt aaaggtgaag ttatctctgc tccatgttca
      121 attaaaccag gtgacgaaga cttaactgta aatctgggtg aagtagcaga taccgttctg
      181 aaaagcgacc agaaatcttt ggctgagcct ttcactattc acctgcaaga ctgcatgttg
      241 agccagggtg gaaccactta ttcaaaagcg aaggttactt tcaccacagc gaatactatg
      301 acgggtcaga ctgacctgct gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc
      361 gttggcgtac gtattctgga tagccagagc ggcgaagtga ctttaggtac tccggtagta
      421 attacattca acaatactaa cagctatcag gaactgaact tcaaagctcg tatggaatcc
      481 ccaagcaaag atgctacccc gggcaacgtt tacgctcagg ctgactataa gattgcttac
      541 gagtaatcac attaggccgg gttcgcccgg ccacatcatt aacggattaa tgataagtgg
      601 aacagatgta taaaaaatta aaattaacca caataagcga attgatt
//
LOCUS       DAZUFB010000432          644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZUFB010000432 DAZUFB010000000
VERSION     DAZUFB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.443"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            436..642
                     /locus_tag="ACHY3M_005591"
     CDS             436..642
                     /locus_tag="ACHY3M_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4320243.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKAVRGE"
BASE COUNT          164 a          126 c          178 g          176 t
ORIGIN      
        1 gccagcgact gacctgaaag aagacgctgg ctaaaaggcc ttgtatgttt gtagagactt
       61 atttttcaca gacagcaatg atgcctgtca atatattatc aatatgcgga ttgtttcagt
      121 tacagatgct ttattaagga aaaaaacagc cagcactgac tttcggtggg gaggtgctgg
      181 ctcaaaagga tagttggatt tcacatgata cttatgcctg gcggtatatt ttctgacaga
      241 cagtgacgga tgttgtcaag atattgtgtc atttataacc tgaaccaggg gggccggaat
      301 gttatctggc atttttagca gagcctgaat gccataatca cggctcccgg agttggccgt
      361 cagtgggtga cactggcggc tttttgtttt cctttacttt cattttctgt cggcggtgac
      421 ggagacatac atcagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggta
      481 gggacgggat actggttact gcaactgctg gacaaagtct ctccgtccca gtgggtggca
      541 ataggtgtac tggggagtct gctgtttggc ctgctgacgt atctgactaa cctgtatttc
      601 aaaatcagag aggaccggcg taaggcggtg cggggagagt aaag
//
LOCUS       DAZUFB010000433          643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZUFB010000433 DAZUFB010000000
VERSION     DAZUFB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.444"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..586
                     /gene="espW"
                     /locus_tag="ACHY3M_005592"
     CDS             <1..586
                     /gene="espW"
                     /locus_tag="ACHY3M_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFS4320244.1"
                     /translation="QIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFE
                     NISSDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRL
                     YSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATF
                     LRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          258 a          110 c          108 g          167 t
ORIGIN      
        1 acaaattgca gatgaagtaa aatcacttac atatttgatt ccttcagaaa aaaaagagat
       61 gtggatatat aaaaacaatg gaaaagataa tgcaaaacca aacttaggag agcgagacgt
      121 aagaatgttc gaaaatatta gttctgatga tacagataag ataacaggaa ggaaattttc
      181 agagttaggt gagtatcttt attcaggaaa tgtaataaaa ctcagtcagt tatcaattcg
      241 ttacttaccc aatatcagct caatctcatt aatagagaca aaacagagtt tgttgctaca
      301 tcgattgtat tcagatgaag tacttcagag aaatggaacg cttatcccga caccactaca
      361 tgaagaaaaa tcaattccag ctgacaatat aaaaacaatg ctcaacaaca taccgactta
      421 caaaatgtta ccgccattca cagaaacaca aggtaattgt tcttctggcg cagccacgtt
      481 tttacgcaaa tcaggcgccg aagaaaaaga tattcttgca tgtagccccc gaaattatgg
      541 gctgcatcat aacataaaaa catgggaccc cttggttaga aattaaggat ccaaatatta
      601 tatatttcat ataaagcaag aaaaaaatat tgtctgaata att
//
LOCUS       DAZUFB010000434          632 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZUFB010000434 DAZUFB010000000
VERSION     DAZUFB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.445"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..204)
                     /locus_tag="ACHY3M_005593"
     CDS             complement(<1..204)
                     /locus_tag="ACHY3M_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4320245.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVN"
BASE COUNT          194 a          158 c           88 g          192 t
ORIGIN      
        1 gttgaccgct tgttttattt cggtcaggtt caccggtgaa accggagtcc ggcgcgcctt
       61 acgcaaacac tctgctcgtt tctgtgccgc cacttttctt ttctggtcat cacttagctg
      121 taccatcact tttgcccatc gttcagctgc tctccggtac agtccttttt tctccagaca
      181 ttctgccacg tgatcatgta gcataagtga cctccgatta tctacagact gccatcctga
      241 atttaccttc ccttaatgaa ataacaataa aaaacaaacc acgcaaaaac aataaaacaa
      301 cacacaaaaa aaactaaata ataaacaaaa ataatcacct tattttatta ttttttgagg
      361 gggcaattac tgaacaaaaa acgctgacta tatactcaaa accaaacaac tattctgcca
      421 atcaggtatc atggcaacac acggaattac cgtgtttttg ccttctctgc ccatacaata
      481 cgggcatata cttcattctc tattgtaata tttctatcca tgtgccccac tccatttacc
      541 tgtaaataat attcaaaata tttatcacag aaatcgtttt tggccatgaa ctgagcacac
      601 tataaagtcc ggaactgact ctttgttaaa tt
//
LOCUS       DAZUFB010000435          627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZUFB010000435 DAZUFB010000000
VERSION     DAZUFB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.446"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>627)
                     /gene="wzx"
                     /locus_tag="ACHY3M_005594"
     CDS             complement(<1..>627)
                     /gene="wzx"
                     /locus_tag="ACHY3M_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O111 family O-antigen flippase"
                     /protein_id="HFS4320246.1"
                     /translation="FFILSICFFDVARNNSSFLFAIIICGFFQEVDNLFSSALKGFEK
                     FNVSCFFEVITRVLWASIVIYGIYGNALLYFTCLAFTIKGMLKYILVCLNITGCFINP
                     NFNRVGIVNLLNESKWMFLQLTGGVSLSLFDRLVIPLILSVSKLASYVPCLQLAQLMF
                     TLSASANQILLPMFARMKASNTFPSNCFFKILLVSLISVLPCLALFFFG"
BASE COUNT          273 a          102 c           92 g          160 t
ORIGIN      
        1 accaaaaaag aataacgcaa gacaaggcaa aacagaaatt agtgatacaa gcagaatttt
       61 aaaaaaacaa ttagagggaa atgtgttaga tgctttcatt ctagcaaaca ttggtagtaa
      121 tatttgattt gcagacgcag aaagagtgaa catcaattga gctagttgaa ggcaagggac
      181 ataagaagcc agtttactga cagataaaat caatggtatt acgagcctat caaacaaact
      241 aagtgagacg ccaccagtta attgaagaaa catccatttt gactcattta acaaattaac
      301 aatcccaact ctattaaaat taggattgat gaaacaaccg gtaatattca gacatacaag
      361 aatatatttt agcatacctt taatggtaaa ggctaaacat gtaaaatata agagtgcatt
      421 tccgtaaatg ccatatatta ctatagaagc ccagagcact cttgtaatta cttcaaaaaa
      481 acatgataca ttaaattttt caaaaccttt tagcgcacta ctaaataaat tatcaacttc
      541 ctgaaaaaaa ccacaaataa taatcgcgaa taagaatgaa gaattattcc tcgcaacatc
      601 gaagaaacaa atacttaata taaaaaa
//
LOCUS       DAZUFB010000436          621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZUFB010000436 DAZUFB010000000
VERSION     DAZUFB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.447"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..140)
                     /locus_tag="ACHY3M_005595"
     CDS             complement(<1..140)
                     /locus_tag="ACHY3M_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320247.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..546)
                     /locus_tag="ACHY3M_005596"
     CDS             complement(121..546)
                     /locus_tag="ACHY3M_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4320248.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          139 a          203 c          139 g          140 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
      601 gtcgcttctg ctttcccggt t
//
LOCUS       DAZUFB010000437          620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZUFB010000437 DAZUFB010000000
VERSION     DAZUFB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.448"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..>620)
                     /locus_tag="ACHY3M_005597"
     CDS             complement(14..>620)
                     /locus_tag="ACHY3M_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4320249.1"
                     /translation="KGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASG
                     YFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEK
                     YNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSK
                     EDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF"
BASE COUNT          193 a          120 c           91 g          216 t
ORIGIN      
        1 taaccttaaa tctttagaag catttttctt tataatactt taaataatta aaaatgcctg
       61 ccatataatg cttttgttta aatttatgtt ttgcacaata ccgatactga ttgctattta
      121 caggtttaag taaatcttct ttactccacg gagaagctgc tttggcgatc aaaaaactac
      181 gtgacaccgg atagtcagcc cactcatgcc agggttttgt aggtcccaca tagtgtataa
      241 acacggtgtc atcattgact ggattatcaa ctttgtcttt taattcatag ttaatactat
      301 atcgggtatt atatttctcc gaaataaact tcactttacc attcaataat acattcagta
      361 catcttgatc aaggtgggtg attttactta cccaatctgg gtcacgcaac atttcaatag
      421 cttttgacga aatgttattt agattccact catcaatatt tatcagtaaa aaaccagcat
      481 tgaaatatcc agaagctaac tgtggtgtag ttaataccga ggctcgattt tgccaccatt
      541 caacatctct ttcagctaca actgcggcaa tttcattagt agaaaattga taatctaaga
      601 gttctttaat actacccttg
//
LOCUS       DAZUFB010000438          620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZUFB010000438 DAZUFB010000000
VERSION     DAZUFB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.449"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..313
                     /locus_tag="ACHY3M_005598"
     CDS             35..313
                     /locus_tag="ACHY3M_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFS4320250.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARMLTKEKAPKNGEFFEA"
     gene            348..494
                     /locus_tag="ACHY3M_005599"
     CDS             348..494
                     /locus_tag="ACHY3M_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFS4320251.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            487..>620
                     /locus_tag="ACHY3M_005600"
     CDS             487..>620
                     /locus_tag="ACHY3M_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFS4320252.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     A"
BASE COUNT          208 a          128 c          153 g          131 t
ORIGIN      
        1 ttcaatcaat tgttatctaa ggaaatactt acatatgcaa cttacaagta ctcgcaagaa
       61 agcgaatgca attacaagca acatcctgaa ccgaattgct gtacgtggcc agcgaaaggt
      121 tgctgacgcg ttaggaatta atgaatcgca aatttcgcga tggaaagaca gcttcatccc
      181 aaaaatggga atgcttctgg ctgttcttga atggggtgtt gaagacgagg agttggcgga
      241 actggctaag aaagtagcca gaatgctgac aaaagaaaaa gccccgaaga acggcgaatt
      301 cttcgaggcc tgatgtagaa agactggatc aatccacagg agtaattatg acaaaacaac
      361 tcagtcctta ccaggacaaa attcacaaac acatactacg tgatcgcttc ctgtccagct
      421 tcaagcagcc tggtcgattc cgggctgagt tggaaaaagt gaagctgatg cagaaggaga
      481 aaggtcatga gtaacatatc taatctagcc gaagccagag aggccagaag gctacaacaa
      541 ccgcatcaaa gcagcggtaa ggggtatgcc ttgctgcacc gtaaaattat ggatgtgccg
      601 ttttacaagg acgcagaagc
//
LOCUS       DAZUFB010000439          611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZUFB010000439 DAZUFB010000000
VERSION     DAZUFB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.450"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>611
                     /locus_tag="ACHY3M_005601"
     CDS             <1..>611
                     /locus_tag="ACHY3M_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320253.1"
                     /translation="EAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGR
                     QSDTKEIAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALG
                     LYIRKGDGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNG
                     QLRAVAAGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQD"
BASE COUNT          149 a          144 c          186 g          132 t
ORIGIN      
        1 gaggcgatgg agccggattt tgttcccatg cgcactgatg aatccgccgg actgaatcag
       61 ggggtttttc ttgatgagtg gggaagaccg aaaaaatatc tggtttataa aaattatccg
      121 gtcagcggcc ggcagagtga tacgaaagaa atcgctgccg gaaaaatgat ccacctgaag
      181 ttcactcgtc gtctgcatca gacgcgaggc tcatccatgt tatcgggggt gctgatgcgg
      241 atcagtgccc ttaaggagta tgaggatgcg gaactgacag cggcgcgtat tgctgcggcg
      301 ctgggactgt atatccgtaa aggtgacgga caggactatg aagatccggg gatcaaagag
      361 accgagcggg aagtccatat caccccgggt attatttatg acgatttgcg caagggcgag
      421 gatatcggca tggtcaaatc tgaccgtccc aatcccaacc ttgaaacttt ccgcaacggc
      481 cagttgcgtg cagtggcagc aggcagtcgt ctgagttttt ccagtgcggc gcgtaactat
      541 aacggcacct acagcgccca gcggcaggag ctggtcgagt ccacggatgg ttacctgatc
      601 ctgcaggact g
//
LOCUS       DAZUFB010000440          610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZUFB010000440 DAZUFB010000000
VERSION     DAZUFB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.451"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..>610
                     /locus_tag="ACHY3M_005602"
     CDS             87..>610
                     /locus_tag="ACHY3M_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS4320254.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTT
                     SQFLASVILDNLPPR"
BASE COUNT          152 a          151 c          192 g          115 t
ORIGIN      
        1 gtaacaaaca gcgttctgat gatatcgcgc aatagctgtg caatatccta tcactgcgat
       61 taataatacc aattgagagg aacattatgg gcaaaggtgg cggcagggcg cacacgcctc
      121 gagaggcgaa ggataatctc aaatccacgc agatgatgag tgtgattgat gcgattggtg
      181 agggaccgat agaaggcccg gtgaaggggc tgcagagtat cctggtgaac aaaaccccgc
      241 tgacggacac ggacggtaat cccgtgatac acggtgtgac tgcggtctgg cgtgccgggg
      301 agcaggagca gacaccaccg gaaggctttg agtcctccgg agctgaaacc ggactgggcg
      361 tggaagtgac gaaggcaaag ccggtgacgc gcaccattac gtccgcgaac attgaccgcc
      421 tgcgggttac cttcggggtg cagtcactgg tggagaccac ctcaaagggt gaccgtaacc
      481 cgacgtctgt ccgcctgctg attcagcttg agcgtggtgg taaatggatg acggaaaagg
      541 atgtcaccat taacggcaag accacctcgc agttcctggc gtcggtgatt ctggataatc
      601 tgccgccccg
//
LOCUS       DAZUFB010000441          609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZUFB010000441 DAZUFB010000000
VERSION     DAZUFB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.452"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..321
                     /locus_tag="ACHY3M_005603"
     CDS             <1..321
                     /locus_tag="ACHY3M_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4320255.1"
                     /translation="SLRLKGKPESYVVPLAFVKNPEKTLTVNTGALLTMSVSVNGGTP
                     PYKHAWKKDGQPVEGQTTDTFSKANAQSGDKGAYTCEVTDSAEQPQSITSDACTVTVN
                     GAGG"
     gene            327..>609
                     /locus_tag="ACHY3M_005604"
     CDS             327..>609
                     /locus_tag="ACHY3M_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS4320256.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDRE"
BASE COUNT          146 a          134 c          202 g          127 t
ORIGIN      
        1 tcacttcgcc tgaagggtaa accggagtcc tatgtggtac cgctggcgtt tgtgaaaaat
       61 ccggagaaga cacttaccgt gaataccggt gcgctgctga caatgtcagt cagtgtcaac
      121 gggggaacgc cgccttataa acacgcctgg aagaaggatg gtcagccggt agagggacag
      181 actactgaca ctttcagtaa agccaatgcg cagtcaggtg ataagggggc ttatacctgc
      241 gaggtaacgg attctgcaga acagccgcag agcattacct ctgatgcgtg tacggtaacg
      301 gttaatggtg cgggcggata aggcttatgg caaaagatct gaaaacactg gcgctggcca
      361 gactgtcggg gttccgtcat aaaacggtga aggtgccgga atggggaaat gtcagcgtgg
      421 tgttgcggga gccttcggca gaggcctggt atctgtggca ggaagtgctc aatggtgatg
      481 gagaggatga cgataccctg tcggtggtgg cgaaaacccg ccgtaacctg gaagcggatg
      541 tgacgctgtt ctgcgatgtc ctgtgtgata cggatctgca gcgggtgttc actccggacg
      601 accgtgagc
//
LOCUS       DAZUFB010000442          607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZUFB010000442 DAZUFB010000000
VERSION     DAZUFB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.453"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..607
                     /locus_tag="ACHY3M_005605"
                     /pseudo
     CDS             1..607
                     /locus_tag="ACHY3M_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          191 a          101 c          162 g          153 t
ORIGIN      
        1 tgcaccagcc gtaccggctg ccggggcagc agtatgataa ggagtcgggg ctgtactaca
       61 accggaaccg gtactacgat ccgttgcagg ggcggtatat cactcaggac ccgatagggc
      121 tggagggggg gatggagtct gtatgcgtac ccgctgaatc cggtgaatgg tattgatcca
      181 ttagggttaa gtcccgcaga tgtagcgcta ataagaagaa aagatcaact aaaccatcaa
      241 agagcatggg atatattatc tgatacttat gaagatatga agagattaaa tttaggtggg
      301 actgatcaat ttttccattg tatggcattt tgtcgagtgt ctaaattaaa tgacgctggt
      361 gttagccgat cggcgaaagg gctgggttat gaaaaagaga ttagagatta cgggttaaat
      421 ctgttcggta tgtacggcag aaaagtaaag ctatcccatt ctgaaatgat tgaagataat
      481 aaaaaagact tggctgtaaa tgaccatggg ttgacatgtc catcaacaac agattgctca
      541 gatagatgta gtgattatat taatccagag cataaaaaaa cgataaaggc tttacaagat
      601 gctggct
//
LOCUS       DAZUFB010000443          604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZUFB010000443 DAZUFB010000000
VERSION     DAZUFB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.454"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>604
                     /locus_tag="ACHY3M_005606"
     CDS             <1..>604
                     /locus_tag="ACHY3M_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS4320257.1"
                     /translation="DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGP
                     RGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKAVKA
                     AMDVANEAKTKAEEAAAGGGVPGPKGDKGDTGPAGPAGPKGDKGERGDTGPVGATGER
                     GPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGA"
BASE COUNT          155 a          142 c          243 g           64 t
ORIGIN      
        1 gtgatccggg aggtcctgca ggcccgcaag gtcctaaagg cgaacgagga gaagccggac
       61 cacagggacc gatgggagca cgaggtgagc gtggggagac tggcccccga ggtgaacctg
      121 gtcctgcagg tccgagaggc gaacgaggag agaccggacc tcagggacct cgtggagagc
      181 caggtccggc aggcagcgct gcaaatgtgg ctgatgcaac gacggcacag aagggaattg
      241 tgcagttaag cagcgcaacg gacagtgatg atgaaacgaa ggctgccacc ccgaaagcgg
      301 tgaaagcggc aatggatgtg gcaaatgaag cgaaaacaaa ggcagaagag gctgcagcag
      361 gaggtggtgt tcccggtccg aaaggagata aaggggacac ggggccagca ggtccggctg
      421 ggccgaaggg tgataaggga gagcgcggtg acaccggccc tgtcggggca accggcgaac
      481 ggggaccggc aggtgatgct ggtccggcag gcccgcaggg gccgaaaggt gacaggggag
      541 agcggggaga gaccggtctg acgggaaatg caggtccaca gggtccaaag ggagataccg
      601 gtgc
//
LOCUS       DAZUFB010000444          598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZUFB010000444 DAZUFB010000000
VERSION     DAZUFB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.455"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..138
                     /locus_tag="ACHY3M_005607"
     CDS             <1..138
                     /locus_tag="ACHY3M_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320258.1"
                     /translation="VTFNGQQLTLESLSQIRAGRQEWERRLAAMVSRRRGKPGFKLAR
                     F"
     gene            138..>598
                     /locus_tag="ACHY3M_005608"
     CDS             138..>598
                     /locus_tag="ACHY3M_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4320259.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGA"
BASE COUNT          127 a          133 c          203 g          135 t
ORIGIN      
        1 gtcaccttca acgggcagca actgacgctg gaaagccttt ctcagatccg cgccggacgt
       61 caggagtggg aacgcaggct tgccgcgatg gtgagccgca ggcggggaaa accgggattt
      121 aaactggcga ggttttaatg gcaattattg atgatgtgat cggcgtgttt tcccccgggt
      181 ggaaagcagc cagactgcgt tcaagggcgt taatcatggc ctatgaggcg gtgaaaccga
      241 cccggacaca taaagcccgg cgggaaaatc gctctgctga tcagctcagt aaatacggtg
      301 cggtttccct gcgggagcag gcccgttttc tggatatcaa tcatgacctg gtgattggtg
      361 tgtttgacaa gctggaagag cgggtgattg gtgccagggg aattattgtg gagcctcagc
      421 cattacgaaa aaacggggaa atggcggctg agctggctgc ggatatccgc cgtttgtggg
      481 ctgaatggtc cgtgagtccg gatgtgacag ggcagtatac ccgtcctgtg cttgaacgtt
      541 tactgctgcg gacctggctg cgggatggtg aagtgtttgc gcagatggtc agtggtgc
//
LOCUS       DAZUFB010000445          597 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZUFB010000445 DAZUFB010000000
VERSION     DAZUFB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.456"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..569)
                     /locus_tag="ACHY3M_005609"
     CDS             complement(<1..569)
                     /locus_tag="ACHY3M_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS4320260.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAA"
BASE COUNT          133 a          199 c          159 g          106 t
ORIGIN      
        1 atggcggcat gattggccac cgatgcgcca aagcagcaca gcaggatatc gcccgcctgt
       61 gcagaggaca ggggcacccg gtaaaaacca gtcgccgcca tattgtccag gtaaaggttc
      121 tgaccgttgc gccaccagtc atcctcacgc tcaaaatccg gcagcgttat ccccgccaga
      181 tggtatgcat cccggaacag ggtgtaacag tccgtcaccc cgtgctcaaa acgacgcccg
      241 gtcaggtgcg gcacacagcg gaatttatga atttcccccc ggcagaccag ccaccagggc
      301 agggcactct ttatctgcag ccgccggtcg gcctcgctca gccagggcag accaccaggg
      361 tggctgtgaa ccagcgccac aatctccccc tgcatctctg cctgcagcca gtcttccggc
      421 gcaatacgaa aatacgcctc cggctctgcg gagatattca cgcagggctg gtaccgttcg
      481 ccctccgggg tgcctatcac gaagccgcac gactccgcag gcgcacaccg ccgggcatgc
      541 accagaatcg ctgattcagt ctgtgtcata aaacaggatt tactgcgaaa gtttatt
//
LOCUS       DAZUFB010000446          593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZUFB010000446 DAZUFB010000000
VERSION     DAZUFB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.457"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..483)
                     /locus_tag="ACHY3M_005610"
                     /pseudo
     CDS             complement(1..483)
                     /locus_tag="ACHY3M_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          153 c          145 g          154 t
ORIGIN      
        1 tgcgtttcct gatggcatgc cggatcacac tgttcagcga ctcgatggcg ttcgttgtgt
       61 agatcacttt gcggatatct gccggataag cgaaaaacgt cgccaagtta gtccagtttg
      121 acagctagca ccggcttatc tgcggatagc ggcagtccca ggccgcagca aacgcttcca
      181 gtgcctgctg gcctgcctct tctgtgggag gctaatcgct ttcaggtcgc gagtgacagc
      241 tttgtattcc ttccatgaca cgaagcgcat gctgttgcgc accatatgca cgatgcataa
      301 ctggatgcgg gcctccggga attctttcag gccatacaca caggcgatga ggatatcgtt
      361 cagaccgcgg tttttcagtt cagtcagcac attgaaccag aaattcgccc cttcattttc
      421 ggccagccgc atccccagga gctctttctg acctgcgata ttgatgccca gtgctaggaa
      481 catttttatg ataaaaaatt agtgcaagaa gacaaagaat cgccttgcgc taatgctctg
      541 aaacaggtca ctaatatcat ctaaatagtt gactcatagt gacagcatgt gtt
//
LOCUS       DAZUFB010000447          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZUFB010000447 DAZUFB010000000
VERSION     DAZUFB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.458"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..242
                     /locus_tag="ACHY3M_005611"
     CDS             57..242
                     /locus_tag="ACHY3M_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFS4320261.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            483..>588
                     /locus_tag="ACHY3M_005612"
     CDS             483..>588
                     /locus_tag="ACHY3M_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFS4320262.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYS"
BASE COUNT          190 a          104 c          109 g          185 t
ORIGIN      
        1 gaacagatga ttgatatttt tgggccggag aaacgcagac aggctttgtt gaataaatcg
       61 agcttttgct gagtttaagg atcagatcac tcattttcct gcaaaagctc attgggcgaa
      121 acaatctgac tcagagaacc cggattaaac gtctggcccg cagaaccatc tatttctcac
      181 gcactgtagt acgtaataaa aaagtcatcg gtacttttat caaaaaacat atattctatt
      241 aattattgcc atcacctttt tcttattgac aacgttactc tgctgattgt taaaatctaa
      301 cctgtattca gggttacgat tctatacaat agaacaggga ggggaaagga tgtcatcgtt
      361 aatgagactt tttgttaatg tcaacaatgc tatgaattta ttttagaaaa acaattaagc
      421 tgacgtagtg ttccgtctaa atgacggggc gatattaaca tgattagaac aagaggaatt
      481 ttatgttatc ttcattaaat gtccttcaat ccagcttcag aggaaagaca gctttatcaa
      541 atagtacact tctccagaaa gtttcttttg ctggaaaaga atattctc
//
LOCUS       DAZUFB010000448          587 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZUFB010000448 DAZUFB010000000
VERSION     DAZUFB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.459"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..385)
                     /locus_tag="ACHY3M_005613"
     CDS             complement(<1..385)
                     /locus_tag="ACHY3M_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFS4320263.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHS"
     gene            complement(376..587)
                     /locus_tag="ACHY3M_005614"
                     /pseudo
     CDS             complement(376..587)
                     /locus_tag="ACHY3M_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          180 a          102 c           82 g          223 t
ORIGIN      
        1 gagaatgaac ttctaatgat gtgtgagatt tattctcttt ctcaagttga agaaaaaggt
       61 tatatataac gcttccccaa gattcatttt caacaaaata cacatcaccc ttaatattat
      121 ttgtcccctt gttttcctga attacaatag ctttgcttaa taattgaggg gatgaaaatg
      181 tttcgtagtc tatctttcct gaattactac aaaaaaactc actccaatat gatgctatat
      241 gtcgacacca tatgatttcc ccattgcaat cactaacctt tccatttaga tttatactgg
      301 catctctctt ttttgataaa taagggggat tgttttttga aatatattca acataatttg
      361 atgccgattt gtttattatt ggcatatgtt tgtatccttt ctataaattt tccgcctgac
      421 agtggaaata atatattcta ctgatttttg tagtttaaac gatttatgaa gaattttact
      481 gttgtacagt accagatact cagccagttt cagattgaac atattcagct attcaaagag
      541 caattcttca ttaaactcaa taaattgttc cctcagtgtt cggttaa
//
LOCUS       DAZUFB010000449          580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZUFB010000449 DAZUFB010000000
VERSION     DAZUFB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.460"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHY3M_005615"
     CDS             complement(<1..71)
                     /locus_tag="ACHY3M_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320264.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..418)
                     /locus_tag="ACHY3M_005616"
     CDS             complement(68..418)
                     /locus_tag="ACHY3M_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4320265.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(428..>580)
                     /locus_tag="ACHY3M_005617"
     CDS             complement(428..>580)
                     /locus_tag="ACHY3M_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS4320266.1"
                     /translation="ALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT          126 a          205 c          132 g          117 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcacct cccagccgac agagaatttc cagacgtccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg ccacggatat
      301 ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca gcattacggg
      361 ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat attgccattc
      421 cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggac acagaaaacg
      481 gcagttcccc ctgcacgagg tcttcccgct cagcaagatc cggattccgg tacagcatcc
      541 cggtcagtcg catggcagcc cccttcatcc gggttaatgc
//
LOCUS       DAZUFB010000450          579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZUFB010000450 DAZUFB010000000
VERSION     DAZUFB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.461"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..518
                     /gene="espL2"
                     /locus_tag="ACHY3M_005618"
     CDS             <1..518
                     /gene="espL2"
                     /locus_tag="ACHY3M_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFS4320267.1"
                     /translation="KAYCDVLGNSNLTRGEIIRMLEARNYDGAPGLLLAYQNGDINTI
                     QSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGKLFKKLDTSPY
                     KMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLSEFSGK
                     HFLDVYNYYSN"
BASE COUNT          217 a           79 c          107 g          176 t
ORIGIN      
        1 taaaagcata ttgtgacgta cttggcaata gtaatttgac aagaggtgag ataatcagga
       61 tgcttgaggc gagaaattat gatggtgcac caggattact tcttgcgtat caaaatggag
      121 atattaatac gattcagtct ttttttgact cactaataat gttagatata tcaaaggatt
      181 ttatcgaaga gttattgact gcaaaacatt atgattttac ggggttatca ttagcgatca
      241 gtcatagaca tgatcatgtt gtcaaattat acggaaagtt atttaaaaaa cttgatacca
      301 gtccatataa aatgtcaatt atcctagcat tagcaattga ttgcgagaga aataatgcaa
      361 acataattat tgatagtgag tacaagtcaa ataaggcagt aaaggaatat gtagaaattt
      421 taaaagaatt caatatatgc ccagaaaagg tagcagaata cctaagcgag ttcagcggaa
      481 aacatttcct cgatgtatat aattattatt ccaattgaaa aaaaacataa agtatctatc
      541 acggcactct cagttgagta tcacaggcac atgtatttt
//
LOCUS       DAZUFB010000451          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZUFB010000451 DAZUFB010000000
VERSION     DAZUFB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.462"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..576
                     /locus_tag="ACHY3M_005619"
                     /pseudo
     CDS             1..576
                     /locus_tag="ACHY3M_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          145 a          141 c          179 g          111 t
ORIGIN      
        1 ggcctacaag attggcgttg gccacccgcc ttccagtgac aacgtggagg cgctggagca
       61 gcagtattat tcccagtgtc tgcagacgct gattgagtcc attgagctgt tactggatga
      121 ggcgctggaa acgggggaaa acgagagcac ggagtttgac gtcaccacgc tgctgagaat
      181 ggacagcgaa cggcgcatga aaacactggg tgaatcggtg aaaaatacgc ttctcacgcc
      241 caatgaggcc cgtaaacgtg agaacctgcc gccccttgcc ggcggtgatg cactgtatct
      301 tcagcagcag aactacagtc tggaggcgct gtcccgtcgt gatgcccgtg aggacccgtt
      361 cgcgtcggcc gggaaaacag tttcagcaca actgcctgac ggcgcatctg acggtaataa
      421 ggcaatcagt gaaacagagc atgatgcagt gaaagcgatg ttcaggggga tactgagaaa
      481 atgacggaac gggaactgtc cattattcgt gcactgggcg aagaattctc cacggtgctg
      541 gcggatttac agcgcacatt tgaggggaag atggcc
//
LOCUS       DAZUFB010000452          574 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZUFB010000452 DAZUFB010000000
VERSION     DAZUFB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.463"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..207)
                     /gene="grlR"
                     /locus_tag="ACHY3M_005620"
     CDS             complement(<1..207)
                     /gene="grlR"
                     /locus_tag="ACHY3M_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFS4320268.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQ"
     gene            403..>574
                     /gene="etgA"
                     /locus_tag="ACHY3M_005621"
     CDS             403..>574
                     /gene="etgA"
                     /locus_tag="ACHY3M_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFS4320269.1"
                     /translation="MKKIILSIILISNYCNASDCFEITGKAYNIDPLILKAIAWNESK
                     CKSGIKSKTNKNG"
BASE COUNT          208 a          109 c           77 g          180 t
ORIGIN      
        1 ttgttcaatg tttagcaccg agggaatttc gtaattatat cgatggacat gaagtatgat
       61 gtcctcatct tcagagagaa ccccctgata cacagaagca atatcgccgc cagtgatcat
      121 atttccattt ttaatcagta taccttcccc acaggagtct tcattgctaa taaatataat
      181 gctatagatg ccatccttca taatcataac tcactcagtt tcatactaac ctcactcctt
      241 tcaatttgtt ctatccataa gcaatatcaa gaataatgga gacctttctt tttctccaga
      301 ttgcaatgta atatatcatt aaatatactc atgtttcaac ctgctgtttc cctggttaac
      361 atatgtaatt tctgattaat aatattgagt cacaacgtaa aaatgaaaaa aataatactg
      421 agtatcattc tcatttcaaa ttactgtaat gcttccgatt gttttgaaat tacaggaaaa
      481 gcatataaca tagatccatt aatattaaaa gcaattgcat ggaatgaatc aaaatgcaaa
      541 agcggaataa aaagcaaaac aaacaaaaac ggaa
//
LOCUS       DAZUFB010000453          563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZUFB010000453 DAZUFB010000000
VERSION     DAZUFB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.464"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(382..489)
                     /locus_tag="ACHY3M_005622"
     CDS             complement(382..489)
                     /locus_tag="ACHY3M_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4320270.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
BASE COUNT          123 a          153 c          136 g          151 t
ORIGIN      
        1 tgttctggtt caagattagc ccccgttctg ttgtcaggtt aaacctctca acgtgcgggg
       61 gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca ttgcgcctca
      121 ccggataacg ccggcttgat gtggatacta cgtctcaatt catcttcact tcatccctga
      181 aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc gtttcatgag
      241 ggctctggat gatgcttcca gctcgggttg ccaatgttta ctggcggaag agataaagac
      301 aaaaaaggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg ccatcacggc
      361 agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa tcgctgcggt
      421 aataattccc gccaggatcg atgctgccag gtcgtgccag aaaatcatgg caaactgcgc
      481 gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct tgaattgttg
      541 atggttcaag attagccccc gtt
//
LOCUS       DAZUFB010000454          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZUFB010000454 DAZUFB010000000
VERSION     DAZUFB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.465"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..556
                     /gene="espX1"
                     /locus_tag="ACHY3M_005623"
                     /pseudo
     CDS             1..556
                     /gene="espX1"
                     /locus_tag="ACHY3M_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          212 a           98 c           77 g          169 t
ORIGIN      
        1 tataaaacag agttcttaat tttgtttgat gactttttta gaagccataa cttgcctaaa
       61 ccatctccag tccttcatca tttttttcaa tatactcatc tgagagatgc atatttttat
      121 agatgtaaac tcatagaaca tacagttcaa ttttattttt ttaaacacaa aggtatcaca
      181 ttacttagat tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag
      241 atataccaag aatgccatga aaaattcgtt aattttttaa aggcaaactt caaccaagaa
      301 atatacccac agctatatac tcctgaaata ttttatgagg cctgcagaaa tctccagtca
      361 ttttatgatc atcaggaaac gtcacaaaac gcaaaataca gtgcaataga caagaaaaaa
      421 gtcatcttaa ccaggaaatc aaaaagctta ttaaaaaaaa tattttccct gagctataca
      481 atgagcaatg caataaaatc ccctcctcat cgatagatga taaccaggga ataatgtggc
      541 agaactttaa aactag
//
LOCUS       DAZUFB010000455          553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZUFB010000455 DAZUFB010000000
VERSION     DAZUFB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.466"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..>553
                     /locus_tag="ACHY3M_005624"
     CDS             24..>553
                     /locus_tag="ACHY3M_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4320271.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALT"
BASE COUNT          115 a          145 c          163 g          130 t
ORIGIN      
        1 gactgttgtg atgaggccga tatgtgaatc aggtgaacga gagccatagc cgcgcatccg
       61 atatctggcg cgaagtggcc tcgctgtttc gcccacccag ccggttacca gtagcggaag
      121 ccatcaggcg ttatatgcgg gttccacggg gagccaatac ttccggtccg tgggagtcat
      181 cgctgacgcc ctatatgatt gaccccatta atacattatc agcccgtgaa tatgacgcgg
      241 tggtgtttgt gggacctgcg cgaaccggga aaaccgaagg gctgattgat ggctggattg
      301 tgtacggcat catctgtgat ccggcggata tgctggtggt gcagatgact gagacgaagg
      361 cgcgtgagca ttccagaacg cgtctttcca ggacgtttcg ccacagtccg gaggtcagca
      421 agcgcctcag tccttcccgt aatgacaaca acgtccacga taaaatgttt cttgacggct
      481 ccttcctgaa gattggctgg ccgtcgatca ccgtcttttc ctcttcggat taccgtcgtg
      541 tggcgctgac gga
//
LOCUS       DAZUFB010000456          546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZUFB010000456 DAZUFB010000000
VERSION     DAZUFB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.467"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>546
                     /locus_tag="ACHY3M_005625"
     CDS             <1..>546
                     /locus_tag="ACHY3M_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4320272.1"
                     /translation="NEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVER
                     EGPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDT
                     AEDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGI
                     ETRDSVNQNQQESEQNDQKAEQ"
BASE COUNT          188 a          121 c          134 g          103 t
ORIGIN      
        1 aatgaagaaa cagacctggg cactgttgat gttctccagt catcagaaac acaaactgat
       61 gcagtggaaa ctgaggtatc tgatatccca aaaaatgaaa ccgcgccgga agctgaacca
      121 tctgtagagc gtgaggggcc gttctatttc ctcttcgcag ataaggacgg agaaaaatac
      181 ggtcgcgcaa acaaactttc tggtctggat aaggcactgg ctgctggcgc cactgaaatc
      241 acaaaagaag aatattttgc ccgaaaaaat ggcacataca cgggcttacc gcaaaatgta
      301 gataccgctg aagattcaga acaaccagag ccgataaaag ttaccgctga cgaagtaaac
      361 aaaattatgc aggcagccaa tatcagccag cctgacgccg ataagttgct tgctgcatca
      421 cgtggtgaat ttgttgaagg gattagcgac ccgaatgatc cgaaatgggt aaaggggatt
      481 gaaacccgcg attctgtaaa ccagaaccag caagaatcgg aacagaacga ccagaaagcg
      541 gaacaa
//
LOCUS       DAZUFB010000457          545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZUFB010000457 DAZUFB010000000
VERSION     DAZUFB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.468"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>545
                     /locus_tag="ACHY3M_005626"
     CDS             <1..>545
                     /locus_tag="ACHY3M_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFS4320273.1"
                     /translation="ILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQ
                     YLLRKFKKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTSKPAIPMHASNTV
                     PADYQQITTDKEYNNIISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRI
                     RALNDKRKNQIRTFWRKAGVIT"
BASE COUNT          188 a          123 c          113 g          121 t
ORIGIN      
        1 atcctcaaag caaagcatac gcctgagtat gtaatgactg acgcaggaga aattctggta
       61 ggcagaggga agctacttgg cggtagaaac tctctggcgt ttgaaacagg actcaaacca
      121 gatcgcgttc agtacctgct tagaaagttc aaaaaactcg gcatgattga ctgggtttca
      181 cacggtaaat tctcagtttt ctcggtagag aaatatgacg attatcagtc aaattttgta
      241 ccagcagatt accagcaaat taccacctca aagccagcaa taccaatgca tgcaagcaat
      301 actgtaccag cagattacca gcaaattacc acagataaag aatataataa tattatctct
      361 aatactgacg tattagagag tgccacagca gacaaaaagt ctgacaagaa aaaaccttcc
      421 gtcagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc gccaagaatc
      481 cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa agccggagtg
      541 ataac
//
LOCUS       DAZUFB010000458          543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZUFB010000458 DAZUFB010000000
VERSION     DAZUFB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.469"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..504)
                     /locus_tag="ACHY3M_005627"
     CDS             complement(<1..504)
                     /locus_tag="ACHY3M_005627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4320274.1"
                     /translation="MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNN
                     PALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTD
                     ISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAV
                     AEKCGYSS"
BASE COUNT          159 a          130 c          102 g          152 t
ORIGIN      
        1 gcttgaataa ccgcattttt ctgctacagc ctgtccatat ccatgccggg aacataacat
       61 attcacagca acacgcatcc gctcttccag caacaagcga ctgaacatgt gcccttcatt
      121 tttcagtttt atctttaaca aactctcact catatgcagg cgtaacgcaa tcgcaccaag
      181 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatactgga
      241 taaacatcca cttaaaaata ataatatccg ttcatctgat tcaaacagcg acaggcatgc
      301 catcataaga aacatatccg tggtatctcc ggaaaatctc tggctggtaa ttaaagcctc
      361 agccaacgca gggttgttgg gttccagtga cagataaagc ggaacgtcag tcagatgagt
      421 tcttgtcagc ttatgctgaa tttccagata ttgacttact atggaatggt ttatatcgaa
      481 aattttaact ttgccataat gcataaggaa aagcgccctg atgcatttgg tggccagaac
      541 gac
//
LOCUS       DAZUFB010000459          527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZUFB010000459 DAZUFB010000000
VERSION     DAZUFB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.470"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..331
                     /locus_tag="ACHY3M_005628"
     CDS             92..331
                     /locus_tag="ACHY3M_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4320275.1"
                     /translation="MCGCGKHDDRENGELLTAQLRLGPADILESDENGIIPEQARVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          151 a           88 c          169 g          119 t
ORIGIN      
        1 tggcaggatg ttaagggact ggatgaagtg ggcagtgata caggcagaac aggagaatga
       61 catgaacata ctaaaaaaac ttatgcagcg tctgtgcggt tgcggaaagc atgatgaccg
      121 tgaaaacggg gagttactta cagcacagct gcgactggga ccggcagaca ttctggagtc
      181 agatgagaat ggcattatcc cggagcaggc cagggtaatc acgcaggtgg tgatactgga
      241 tgcggataaa aagcagatac agtgcgtggt aagaccgctg caaattctgc gtgctgacgg
      301 gacgtgggaa aatattggcg ggatgaaata gtcgacagct tcacaaaaac cggagtccgg
      361 ctccggtttt tgttgtcatg tccggtggat gtttgttatg actccctgtg tttggaatga
      421 atatttaata acaggtgtct ggaaatatag gggcaaatct actggatagg ctattggggc
      481 gtgagaatcg aatggagaga agggctgtgg ctctggaaag gcaatta
//
LOCUS       DAZUFB010000460          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZUFB010000460 DAZUFB010000000
VERSION     DAZUFB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.471"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /locus_tag="ACHY3M_005629"
     CDS             <1..162
                     /locus_tag="ACHY3M_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFS4320276.1"
                     /translation="FLILFIVLMIPISPLILVWIIGKIIEPVIELYNDVVWASFNTLH
                     NKINPYKEN"
     gene            164..427
                     /locus_tag="ACHY3M_005630"
     CDS             164..427
                     /locus_tag="ACHY3M_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320277.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            438..>524
                     /locus_tag="ACHY3M_005631"
     CDS             438..>524
                     /locus_tag="ACHY3M_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320278.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMEL"
BASE COUNT          164 a           95 c          114 g          151 t
ORIGIN      
        1 tttctcattc tgtttatcgt gttgatgata ccgatatccc cgttaatcct agtctggata
       61 atcggaaaaa taattgagcc agttattgaa ttgtataacg acgtggtatg ggcgtcattc
      121 aacacactgc acaataaaat taatccgtat aaggaaaact gatatggcaa ctttgacaaa
      181 aaaagaacgg gcatggttga acgaattaca ggaagttctt gatcgctgtc catcaccgaa
      241 aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc tacgccgcat
      301 ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg ttgctgttca
      361 tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg aaagcactgc
      421 gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg ttcattaaaa
      481 atccgcttga aaacgggctt acccgtggtg aacaaatgga actg
//
LOCUS       DAZUFB010000461          515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZUFB010000461 DAZUFB010000000
VERSION     DAZUFB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.472"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..436
                     /locus_tag="ACHY3M_005632"
     CDS             <1..436
                     /locus_tag="ACHY3M_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4320279.1"
                     /translation="LGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALW
                     GAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNM
                     IAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          117 a          118 c          180 g          100 t
ORIGIN      
        1 tctgggtgag ggggcggtca tccacattgt gccgcgactg gccggggccg gaaagggcgg
       61 actgcagatt gtgctggggg cagcagccat cgtgggctct ttcttcaccg ccggcgcaac
      121 gatggcgttg tggggcgcag ccctgagtgc cggagggctg actgccacca cgatgctgtt
      181 ctcactgggt gccagcatga tactgggcgg tgtggcccag atgctggcac cgaaggcaaa
      241 aacaccggag tacagggcga cggataacgg taaacagaac acgtattttt cgtcactgga
      301 taacatgatt gcccagggga acccgatgcc ggtgccttat ggtgaaatgc tggttggttc
      361 acgacggata tcccaggaca tcagcacccg tgatgagggc ggagacggga aagtggtggt
      421 tatcgggcgg ggatgaaaat aaaaaaatcc cgcagagtta gcggagctgc gggagagaac
      481 gatgaagatt aacgttatgg agttattttt caggc
//
LOCUS       DAZUFB010000462          512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZUFB010000462 DAZUFB010000000
VERSION     DAZUFB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.473"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /locus_tag="ACHY3M_005633"
     CDS             complement(<1..79)
                     /locus_tag="ACHY3M_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4320280.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDN"
     gene            complement(91..222)
                     /locus_tag="ACHY3M_005634"
     CDS             complement(91..222)
                     /locus_tag="ACHY3M_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320281.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            complement(317..>512)
                     /locus_tag="ACHY3M_005635"
     CDS             complement(317..>512)
                     /locus_tag="ACHY3M_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4320282.1"
                     /translation="LLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKF
                     TPKGVQWIAGLLAAWRATAA"
BASE COUNT          154 a          148 c          100 g          110 t
ORIGIN      
        1 cgttatcgcg gtaatgattc agccccagac taagcgcacc acagaccacc agcagaataa
       61 cggtaaacgc ggaaagcatt cggtttatgc tcaccccacc agccctgccg aagtcaacgc
      121 catccaggtt atggaaagaa aaagaacaac cagcattagt gaaaatgaaa taccgacgat
      181 tacacaaagg cccttcgcca gcattatgag tttgtctgac atctttaccc cttaatagca
      241 gtaattaacc gggcaaccac ccataaaaac ggaatcagcc agaccagcaa aaatttccag
      301 tccgttttta tcatcttcat gctgcggtag ctctccatgc agcaagcaga ccagcaatcc
      361 actgaacacc ttttggggtg aatttaacct gcgtaaaagc atgaccattg cccgcctcgc
      421 ccgttttcac gctaaaccgc cccgcatcca ggtgatgcga gtaaggcgtc atttttccag
      481 cgaggcgata cattattccg ttctccaaca aa
//
LOCUS       DAZUFB010000463          499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZUFB010000463 DAZUFB010000000
VERSION     DAZUFB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.474"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>499
                     /locus_tag="ACHY3M_005636"
     CDS             <1..>499
                     /locus_tag="ACHY3M_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320283.1"
                     /translation="ILICNDKDSQTIVDKRIGAHFWIPVPRKLLTTLQALIDLELITN
                     ISFRIDNITDYIPFFEDMEVGSPLKLNVADLPSLSKFYSIDNYANSFWVHHDSRERSI
                     TFEELRDDFQMVNDDVITQVIHLEYFNLNNAFYIQHLDHELISYTLEQYEKKLSDSKI
                     KGYKKT"
BASE COUNT          183 a           85 c           74 g          157 t
ORIGIN      
        1 acattttaat atgtaatgat aaagatagtc aaacaatagt agacaaacga ataggtgccc
       61 atttttggat acctgtccca cgaaaactat taactacact tcaggcttta atagacttag
      121 agcttatcac taacatttca tttagaatag ataacataac cgactacatt ccattttttg
      181 aagacatgga ggttggttca cctctaaagt tgaatgtggc agatctcccc tcactttcaa
      241 aattttattc tattgataac tacgcgaata gcttttgggt tcatcatgat tcaagagaga
      301 gaagtatcac ttttgaagaa ttaagagatg actttcagat ggtaaatgat gatgtcatta
      361 cacaggttat tcaccttgag tatttcaacc ttaataatgc attttatata caacatttag
      421 accatgaact catatcctat acgttagaac aatatgagaa aaaattatca gattccaaaa
      481 tcaaaggcta taaaaaaac
//
LOCUS       DAZUFB010000464          498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZUFB010000464 DAZUFB010000000
VERSION     DAZUFB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.475"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..197)
                     /gene="yrhD"
                     /locus_tag="ACHY3M_005637"
                     /pseudo
     CDS             complement(42..197)
                     /gene="yrhD"
                     /locus_tag="ACHY3M_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
     gene            complement(292..>498)
                     /gene="yrhA"
                     /locus_tag="ACHY3M_005638"
     CDS             complement(292..>498)
                     /gene="yrhA"
                     /locus_tag="ACHY3M_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFS4320284.1"
                     /translation="NEFYRNNDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNI
                     GTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          161 a           79 c           73 g          185 t
ORIGIN      
        1 ttatgatttc tttttcagaa cgtcgaacgg gattttctta tttaagatat cagaatctta
       61 tgattcgggt aaaattctaa ataaatcaac atgtcattaa aatcatcata gccattagct
      121 atttcggcta aaatagagac tacatgtctt cggtccatct cacttaagga gtgtagttac
      181 gttgtaagtt tttccatagc ttgcactgct aaatttcgaa caaggaattt tctgctggta
      241 atctctaaaa agatggcatg gtttacaatg atttttgttt ccttttgatt attatgaaca
      301 actgtccatg atttcgttta agaatgaaga gaaatcacta aacgaactga atatattttc
      361 tgtgccaata ttatctctaa tttcaaaaaa gttactttta atgtcataag taaatattga
      421 aatgctatca tccccgatca ctaaccgttc ttgtagatca gggtttatga aatcatcatt
      481 atttctataa aattcatt
//
LOCUS       DAZUFB010000465          487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZUFB010000465 DAZUFB010000000
VERSION     DAZUFB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.476"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>487
                     /locus_tag="ACHY3M_005639"
     CDS             <1..>487
                     /locus_tag="ACHY3M_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320285.1"
                     /translation="LDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNY
                     VKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLS
                     NEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQLL
                     YL"
BASE COUNT          176 a           88 c           80 g          143 t
ORIGIN      
        1 actcgacatc aggaacacat atcgcccgga taatgagatg gcattttact atcatattca
       61 ttcttttctc actcagatac cggataatga ggatgatata tatcatgctg caaggacata
      121 tctgcgaaat tacgttaagt tatgtttatc cggatacacg ccagcgaatg cgcatttcaa
      181 agatatcttt gatggcgtat atgaattcat tcgtaatatt cgcaaaaaca gtacaccagg
      241 aaaaacaaaa cttatcgcaa ctatcaacac atgcaaagaa acctgtaaac atctgcttta
      301 tttaagtaat gaagacaagg aaaaaataat ttctgactta gataaagttc aggttgcatg
      361 ttattatctc actatattac tggctttcga aagacgaact tcattaacaa gcaccctggc
      421 aactttatat aaaatgctga taagcgaaag agaagtttca gaatatgaat gccagttatt
      481 atattta
//
LOCUS       DAZUFB010000466          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZUFB010000466 DAZUFB010000000
VERSION     DAZUFB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.477"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..257
                     /locus_tag="ACHY3M_005640"
     CDS             <1..257
                     /locus_tag="ACHY3M_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS4320286.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT          125 a          117 c          133 g          110 t
ORIGIN      
        1 gtggcggtca ggtgcttgct gttccgccag cgtacacaag ccagcgttgc gcgtgctgtg
       61 gtcatacagc gaaagaaaat cgcctgtcac aaagtcaatt cagatgccag gtatgtggat
      121 atacagcgaa cgccgatgta aacggcgctc gtaacatttt agcggcgggg cacgccgttc
      181 ttgcctgtgg agagatggtg cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga
      241 ttcaggcgac agcctgaacg tagcagggat cctcgtcatt cagggcgtgg aggatgtcaa
      301 gacacggaaa acgaacatga tgtttgtggt tggtaattat cactgatcgt tgatggcgca
      361 atttctgtgc catccgcaaa aaaatcctcc gtgatgctcg tcacaaatct tgccctcccg
      421 atgttgtgct atgcttttta tcagcgacta acccgctaat gcaaaacaga taagtgagga
      481 aacaa
//
LOCUS       DAZUFB010000467          484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZUFB010000467 DAZUFB010000000
VERSION     DAZUFB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.478"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..484
                     /locus_tag="ACHY3M_005641"
                     /pseudo
     CDS             174..484
                     /locus_tag="ACHY3M_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          148 a          107 c           98 g          131 t
ORIGIN      
        1 cactcttgac gcccgtggaa tatattagag aacaaaaatt tcaatatgtg gtggacgcat
       61 acagaggatt gacagcattc ccatactaat tgttaagatt caaattgaag tatatctatc
      121 tgtcattttg caaatgatat gcggtagctt tacctaacta tcatgaacat ttcgaaaaat
      181 catccacaaa gccccgaaga ataaagcact atcgcccgat aaagacctgt aagctcagtg
      241 gcatggatac tattgagctc agaatggcga gctgtaccgt tatttcatta ccatgctcga
      301 cgaacacagc aactactctc tggccgtact atcgcttaaa agtgatatca tcaggcattt
      361 cttctgctgt gtagcccagt tgttccttgt tggtatcaac cagatcatca ctgatgatgg
      421 gcaaagagtt ctcgggaaaa ttcgacaaaa cgctacagga agccaccgtc gggttcgtat
      481 ggac
//
LOCUS       DAZUFB010000468          484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZUFB010000468 DAZUFB010000000
VERSION     DAZUFB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.479"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..167
                     /locus_tag="ACHY3M_005642"
     CDS             <1..167
                     /locus_tag="ACHY3M_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320287.1"
                     /translation="ERSKRYPQRFALKTTPPVGNVQGTGEIEITIQTNGLRRRVKATR
                     ISGCTVYWEV"
     gene            169..450
                     /locus_tag="ACHY3M_005643"
     CDS             169..450
                     /locus_tag="ACHY3M_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFS4320288.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
BASE COUNT          122 a          108 c          153 g          101 t
ORIGIN      
        1 tggaacgcag taagcgctat ccgcaacgtt ttgcactgaa aaccacgccg ccggtaggca
       61 acgtgcaggg aaccggtgaa attgaaatca ccatacagac caacggcctg cgccgccgtg
      121 tgaaggccac ccgcatcagc ggctgcacgg tttactggga ggtgtgaggt gaaaaaaaat
      181 ctcattgcat gggcgtggtc gagtggtctt attgagtttg gttacgtcct gccggaaggt
      241 gcattgccga tagttgccgg aaagcctgcc acggtacggc atgtgattga ggttatggcg
      301 cgtcatggac gtgatgaaca ggagcagtta ctggttccgg ggataccgga agcggtgacg
      361 gaggaagaag cctttaatgc catgattcgg ttctgccgtg aggtcagacg ccgggtcagt
      421 tatccaaaca gaacgaggac cagaggatga gtaaagtcgt acgcattatt ttcgaataca
      481 agga
//
LOCUS       DAZUFB010000469          469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZUFB010000469 DAZUFB010000000
VERSION     DAZUFB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.480"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>469)
                     /gene="espL2"
                     /locus_tag="ACHY3M_005644"
     CDS             complement(<1..>469)
                     /gene="espL2"
                     /locus_tag="ACHY3M_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFS4320289.1"
                     /translation="LGIKIKNDKENKFVINFYDPNQTATHKRVFFCTNNICDIINLTA
                     YDFLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLPDNILQGVVINFAMGAGLR
                     EIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQNGHDNVIDEYGTL"
BASE COUNT          149 a           80 c           62 g          178 t
ORIGIN      
        1 attaaagtac catattcatc aattacatta tcatgtccat tttgtagagc taaaagcaac
       61 ccaggaacat tattaacatt ttttgattca cacaagattt tcatctgaga ttttgtaaga
      121 tctgtaaatc gagtatcgtt atataccttt tttattatct ctcttagtcc tgctcccata
      181 gcaaaattaa taacaacacc ttggagaata ttatcaggta atttttttat aaacacatta
      241 ttgttgtcat tcgattttgt tttatccaca aaaagtgaca gtgtgtcttc ttttagccca
      301 taacatttca gacattgctc actaagaaaa tcatatgctg tcaggtttat tatatcacaa
      361 atgttattag tgcaaaaaaa tactcgttta tgcgtcgcgg tctgattggg atcatagaaa
      421 ttaataacaa acttattttc cttgtcgttt tttattttaa tgcccagag
//
LOCUS       DAZUFB010000470          466 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZUFB010000470 DAZUFB010000000
VERSION     DAZUFB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.481"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(106..387)
                     /locus_tag="ACHY3M_005645"
                     /pseudo
     CDS             complement(106..387)
                     /locus_tag="ACHY3M_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          123 a          146 c          109 g           88 t
ORIGIN      
        1 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
       61 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg accccaaaca ggaaacgcag
      121 ctgaaacggg aagctcaaca cccactgacg catgggttgt tcaggcagta cttcatcaac
      181 cagcaaggcg gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg
      241 cttacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
      301 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
      361 caattccttt ccctgctctg ccataagggg tctgacgctc agtggaacga aaactcacgt
      421 taagggattt tggtcatgag attatcaaaa aggatcttca cctaga
//
LOCUS       DAZUFB010000471          462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZUFB010000471 DAZUFB010000000
VERSION     DAZUFB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.482"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..460)
                     /locus_tag="ACHY3M_005646"
     CDS             complement(<1..460)
                     /locus_tag="ACHY3M_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320290.1"
                     /translation="MSSDLVRRKMNDIPESPTGNNLEALAPGIEKLKQTSIEMVTLLN
                     TLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTINEP
                     LKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRNVDL"
BASE COUNT          138 a           79 c           84 g          161 t
ORIGIN      
        1 ctaaatcgac attacgtaag tccagagagg atagatcaac gatatctcca gttccacaac
       61 taaagattac gttcaacctc ctgatttcat tgtaaatata tttcaatgga caattaacat
      121 tgatactgcc tgtggatatt ttctggttct ttaatggttc attaatggta agttcgcagg
      181 tattgtccga tcgttgaata atttgggcgt tataacctaa aaaatccaga cgaagagacg
      241 agtcattata aagaattaca ttttgtaagt acgctaattc tttttgaaaa tcaccagtga
      301 taatgcattt tccaccaggt tgtaacgtgt taagtaaagt gaccatttca atagaggtct
      361 gttttagttt ttctatacct ggtgctagtg cttcgaggtt atttcctgtt gggctttcag
      421 gaatgtcatt catttttcga cggactaaat cactactaat at
//
LOCUS       DAZUFB010000472          461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZUFB010000472 DAZUFB010000000
VERSION     DAZUFB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.483"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            116..406
                     /locus_tag="ACHY3M_005647"
     CDS             116..406
                     /locus_tag="ACHY3M_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFS4320291.1"
                     /translation="MNIDTTITIDTLLNTGLALLGWLYIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
BASE COUNT          143 a           99 c          120 g           99 t
ORIGIN      
        1 tgtcagcgaa agaagagttg gctaatgaag cgcatccgat tgatggcctg tctggtgaat
       61 atgcggagaa atcccttgaa gaatgggcgg aacagattcg caaaggaagc agccagtgaa
      121 tatcgacacg acaataacga tcgatacgct cctaaatacc ggtctggcac ttctcggttg
      181 gctttacatc atgtcccgca catggcgatg gctgggttcc attttcctaa aacagtggaa
      241 aaaacggcgc aaacaggaac tacgccagaa ggcattagaa gcgttctatg acgcatttga
      301 gcttagccgc attgaaccag gtacaacagc caggatagcg acaaaaggcg acctgatgat
      361 agtgatgttc cgacaggaga gagcagagaa aggggaatca gcatgaaatt ttccaaattt
      421 tctgagttgg tgaatcgtat tttgtccaac aaccacagcc a
//
LOCUS       DAZUFB010000473          458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZUFB010000473 DAZUFB010000000
VERSION     DAZUFB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.484"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..>458
                     /locus_tag="ACHY3M_005648"
     CDS             217..>458
                     /locus_tag="ACHY3M_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS4320292.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          150 a           95 c           76 g          137 t
ORIGIN      
        1 cacagaatgc gcataaaaat aacagcataa aacaccttac caccacccaa gaatttcata
       61 ttgcattgtt tttcaatgaa aaaatattat tcgcgtaata tctcacgata aataacatta
      121 ggattttgtt atttaaacac gagtcctttg cacttgctta ctttatcgat aaatcctact
      181 tttttaatgc gatccaatca ttttaaggag tttaaaatgg ataagaagca agtaacggat
      241 ttaaggtcgg aactactcga ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa
      301 tcaaaacgtt ttccgctgca cgaaatgcgc gacgatgtcg cattccagat tatcaatgac
      361 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
      421 gacgaaaatg tccacaaatt gatggattta tccattaa
//
LOCUS       DAZUFB010000474          458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZUFB010000474 DAZUFB010000000
VERSION     DAZUFB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.485"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>458
                     /locus_tag="ACHY3M_005649"
     CDS             <1..>458
                     /locus_tag="ACHY3M_005649"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4320293.1"
                     /translation="DLNEFSYKEPPKEKITFIFIARLLREKGIFEFIEAAKFVKTTYP
                     SSEFVILGGFESNNPFSLQKNEIESLRKEHDLIYPGHVENVQDWLEKSSVFVLPTSYR
                     EGVPRVIQEAMAIGRPVITTNVPGCRDIINDGINGFLIPPFEINLLAEKM"
BASE COUNT          161 a           58 c           92 g          147 t
ORIGIN      
        1 gatcttaatg agttttcata taaagagcca ccgaaagaga aaattacctt tatttttata
       61 gcaaggttat taagagagaa agggatattt gagtttattg aagccgcaaa gttcgttaag
      121 acaacttatc caagttctga atttgtaatt ttaggaggtt ttgagagtaa taatcctttc
      181 tcattacaaa aaaatgaaat tgaatcgcta agaaaagaac atgatcttat ttatcctggt
      241 catgtggaaa atgttcaaga ttggttagag aaaagttctg tttttgtttt acctacatca
      301 tatcgagaag gcgtaccaag ggtgatccaa gaagctatgg ctattggtag acctgtaata
      361 acaactaatg tacctgggtg tagggatata ataaatgatg ggatcaatgg ctttttgata
      421 cctccatttg aaattaattt actggcagaa aaaatgaa
//
LOCUS       DAZUFB010000475          454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZUFB010000475 DAZUFB010000000
VERSION     DAZUFB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.486"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>454
                     /locus_tag="ACHY3M_005650"
     CDS             <1..>454
                     /locus_tag="ACHY3M_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4320294.1"
                     /translation="MNVNFDKLSPEVQNAVLVKFGTCENITVDMAIDAQELLQEDVAT
                     FDGHIVEALMKTPEINAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAER
                     KETGKYTSVVDLASARVNQQHTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          145 a          104 c          112 g           93 t
ORIGIN      
        1 cgatgaacgt caattttgac aagctgtccc ctgaagtaca gaacgcggtc ctggttaagt
       61 tcggtacatg tgaaaacatc accgttgata tggcgattga cgcgcaggaa ttactgcagg
      121 aagacgttgc tacctttgac gggcatatcg ttgaagcact gatgaaaacg cctgaaatta
      181 acgctatgta tccggaacgc aaactgttcg ctatcggatg ggttaaacac aaatgtaatc
      241 cgggtgccaa atggcccgaa attcaggctg aattacgtaa ctggaaaaaa cggcaggacg
      301 cagagcgcaa agagactgga aaatacacgt ctgttgttga tctcgccagc gccagagtca
      361 atcaacagca cactgaaaac tcagcaggaa aaatcaaccc agtcactgcc gccatttgtc
      421 gcgaatacaa gcagacatgg aaaacgctgg atga
//
LOCUS       DAZUFB010000476          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZUFB010000476 DAZUFB010000000
VERSION     DAZUFB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.487"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>451)
                     /locus_tag="ACHY3M_005651"
     CDS             complement(<1..>451)
                     /locus_tag="ACHY3M_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320295.1"
                     /translation="AESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDK
                     VEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPA
                     ALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSA"
BASE COUNT           69 a          160 c          110 g          112 t
ORIGIN      
        1 gcacttttaa cctgcgaaaa actgtccgtc gcactttccg cccactcgcc ccagccggac
       61 ttcagcccgg ccatccagct tccacgaagc tgctcctccg cagaccaggt gttcttcagt
      121 gccgatgtgg ccttcgccag cgcagccgga ttatcaccgt acacgtcccg aagacgctgc
      181 gcttcagact cccgctgcgc ctgacggtca gtgagaccgc gggcttttgc gctgatggcg
      241 gcctgcttcg cgctctgctg ctcttcaaac cgcaccgcct gctgtgccag ctcattcagg
      301 cgtttctggt attcaacctt gtcgcccagc tcagccagct ggcgtttgta ctccagcgtc
      361 tcgtctttat gcgccagcag ggatttttcc tgctcagata actgtcgttt cgtggcagcc
      421 tctttcagga ccgcatactg actttccgcc t
//
LOCUS       DAZUFB010000477          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZUFB010000477 DAZUFB010000000
VERSION     DAZUFB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.488"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..204)
                     /locus_tag="ACHY3M_005652"
     CDS             complement(<1..204)
                     /locus_tag="ACHY3M_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4320296.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVN"
BASE COUNT          150 a          112 c           67 g          122 t
ORIGIN      
        1 gttgaccgct tgttttattt cggtcaggtt caccggtgaa accggagtcc ggcgcgcctt
       61 acgcaaacac tctgctcgtt tctgtgccgc cacttttctt ttctggtcat cacttagctg
      121 taccatcact tttgcccatc gttcagctgc tctccggtac agtccttttt tctccagaca
      181 ttctgccagg tgatcatgta gcataagtaa cctccgttta tctacagact gccatcctga
      241 attaaccgtc cttaataaga caacaataaa aaactaatta tataaaaaca ataaaaataa
      301 tatacaaaaa aaaactaaac aacaaagaaa agcaaatcat atctggcatt ttaattgaaa
      361 gagcaattac cgaacaaaag acgctgacta tatactcaaa accaaacaac aattctgcca
      421 atcaggtatc atggcaacac acggaattac c
//
LOCUS       DAZUFB010000478          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZUFB010000478 DAZUFB010000000
VERSION     DAZUFB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.489"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>451
                     /locus_tag="ACHY3M_005653"
     CDS             <1..>451
                     /locus_tag="ACHY3M_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS4320297.1"
                     /translation="YDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTSVHTTAANLD
                     IRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPE
                     GGIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA"
BASE COUNT          107 a          156 c          122 g           66 t
ORIGIN      
        1 tatgaccgcg attacacctg gaacgacaac ggcgaactca tccgcatcag cagcccgcgc
       61 cagacccgga gttacagcta cagcaccacc ggcaggctga ccagcgttca caccaccgca
      121 gcgaatctgg atatccgcat cccgtatgcc acagacccgg caggtaaccg cctgcccgac
      181 ccggagctgc acccggacag caccctcagc atgtggccgg ataaccgtat cgcccgtgac
      241 gcgcactatc tttaccggta tgaccgtcac ggcaggctga cggagaaaac cgacctcatc
      301 ccggaagggg gtatccgtac ggatgatgag cgcacccacc ggtaccatta cgacagtcag
      361 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
      421 ctttacgacc cgctgggccg cagggtggca a
//
LOCUS       DAZUFB010000479          441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZUFB010000479 DAZUFB010000000
VERSION     DAZUFB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.490"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..367
                     /locus_tag="ACHY3M_005654"
     CDS             2..367
                     /locus_tag="ACHY3M_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4320298.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT           96 a          121 c          134 g           90 t
ORIGIN      
        1 catggctgtg cttcgtacgt taccgggcag aatcaaaact ctgaacaccc ggcgggtgaa
       61 tattctgaag ggtgaacagc gtcgtgtcag tggcagtgcc cgggtttccc tcaaacgtcg
      121 tatctggcgg agggatgccg gacactgttg tctctgtaga cgcgtggttg acctctgtga
      181 cagtgaactc gatcaccgca ttgcacttca gttcggtggt ggtaatgagg agacgaacct
      241 ctggaccctc tgtactgaat gccatcgcca gaagtcagcg agtgaagcgg cgagtggtat
      301 gcctgatccg acgttgcctg agcttcctga tggcacgctc agggccgacg gaatcactgg
      361 cctgtgacca gacccggggg ggatcatccg gcgaaaaaaa acgatcgccc cggacaccgc
      421 cccccgtctc atgcagagaa a
//
LOCUS       DAZUFB010000480          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZUFB010000480 DAZUFB010000000
VERSION     DAZUFB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.491"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..308)
                     /locus_tag="ACHY3M_005655"
     CDS             complement(<1..308)
                     /locus_tag="ACHY3M_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFS4320299.1"
                     /translation="MLNGISNAVSTIGLHLRGVASRVGSAGVSGFSVAPQTVRLTPVR
                     FHSPFSPGSSNINARTTFNMSHMSQMSQMSRMSQMATSFTPTRPAPPPPTSGQASGSA
                     G"
BASE COUNT          135 a           93 c           88 g          124 t
ORIGIN      
        1 ccggcagacc cggatgcctg tccacttgtc ggtggcggtg gtgccgggcg agtaggagta
       61 aatgaagtcg ccatctgact catacgactc atctgactca tctgactcat atgactcata
      121 ttaaaagtcg ttctcgcatt aatattcgac gaacctggag aaaaagggga atgaaatctc
      181 accggagtaa gacgcacagt ctgaggggct acagaaaatc cagatacccc cgcagagccc
      241 actcgacttg cgacacctct aagatgcagc cctattgtag aaacagcgtt actaattcca
      301 ttaagcataa tttatgtctc tttatatcta attaattggg gttattatta attcatatta
      361 aaactataac tcattaaatt aaatgcatat ggaaatatga atacttttgt acagctgtat
      421 ttatcattct gatatgatga
//
LOCUS       DAZUFB010000481          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZUFB010000481 DAZUFB010000000
VERSION     DAZUFB010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.492"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>440
                     /locus_tag="ACHY3M_005656"
     CDS             <1..>440
                     /locus_tag="ACHY3M_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320300.1"
                     /translation="RVKSLQEKIAGYQYVLANPGWTTGDGFMINHLTSVKTVTEGLAQ
                     ATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQH
                     TEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTER"
BASE COUNT          113 a          105 c          138 g           84 t
ORIGIN      
        1 cgggtgaaat ccctgcagga aaaaatcgca ggatatcagt atgttctggc gaacccgggc
       61 tggacgaccg gtgacggatt catgataaac catctgacct cggtgaagac cgtaacggaa
      121 gggcttgctc aggcaacaga gcagcttgcc gttgagcagt cccgtctggc acagatgcag
      181 gaaaaagcgc agtccattca ggatgtgctt gccgggctgg aagaccgtcg tgtggcgtta
      241 attcgtcagc aggcggcaga gcagaataag gtgtaccagt ccatgctggt tatgaacggt
      301 cagcatacgg aattcaaccg tctgctgggg ctgggtaatg aactgcttca gcagcggcag
      361 ggactggtga atgtgccgtt acggctgcca caggccactc tggatgataa acagcagagt
      421 gccctgacaa aaacagagcg
//
LOCUS       DAZUFB010000482          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZUFB010000482 DAZUFB010000000
VERSION     DAZUFB010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.493"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            10..>440
                     /locus_tag="ACHY3M_005657"
     CDS             10..>440
                     /locus_tag="ACHY3M_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320301.1"
                     /translation="MLFTSFSGSLVSRIPGAVHITLLRHISTINGFDNLMAQPLLSDE
                     PLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGVGTTKNAVFQHIQS
                     LPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNM"
BASE COUNT           89 a          110 c          123 g          118 t
ORIGIN      
        1 tttttagtca tcctgtttac ctctttctca gggagtttag tctccaggat tcccggggcg
       61 gttcatatta cgcttcttcg ccatatatcg acaatcaacg ggtttgacaa tctgatggct
      121 cagccgttgt tgtcagatga gcccctgaca gcgctgatgg atcattatct tgatactgat
      181 gctctggcag acgggctacc gctgtatgtg tcgctgtacc ccacagaagg gggcatgcag
      241 gatattattg actgcattcg tgctgaactg ggtgtcggaa ccacgaaaaa cgccgttttt
      301 cagcatatcc agagcctgcc ccgcggacag cagaaagagg ctctgcttgc gtcagccgcg
      361 ctgcccctgc tgttccgtcc ccgtgaggtt caggggacaa tgttcggtga tggtggtatg
      421 ggaggatggc gaaatatgca
//
LOCUS       DAZUFB010000483          438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZUFB010000483 DAZUFB010000000
VERSION     DAZUFB010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.494"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..436)
                     /gene="nleF"
                     /locus_tag="ACHY3M_005658"
                     /pseudo
     CDS             complement(1..436)
                     /gene="nleF"
                     /locus_tag="ACHY3M_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
BASE COUNT          133 a          103 c           58 g          144 t
ORIGIN      
        1 tgatgcggac atagcagatt ataaacttta catttcgaat cttctttgcc ttgtctactt
       61 tcctcaattt ttactttcaa tgaatgcaga aaaaaagatc cctgatataa ctatccctgt
      121 cctctatcgt cattcccgcc ctcagcgctc cgacaacctc atcagtattt tcatttctca
      181 cctcaaatac actatttctc ttatccatca gcccacttac aaaagcactt agctcttgaa
      241 aaatttcctc aagctcatta ttatttctgg cacatgaata tatagagacc ataacatcaa
      301 gtttatcttg taattcagga gaaagaaagt gattatgatt aagctcactt actgattccg
      361 gagtcgaagg gttacctctt cctgatacat acatccatga ataaagattt gctgaagaac
      421 cacttgttgg taacatat
//
LOCUS       DAZUFB010000484          432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZUFB010000484 DAZUFB010000000
VERSION     DAZUFB010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.495"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>432
                     /locus_tag="ACHY3M_005659"
     CDS             <1..>432
                     /locus_tag="ACHY3M_005659"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000707060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4320302.1"
                     /translation="GRLVEAKDYPNLLNAINHLILSKTSNCNDFILLIAGDGALRNKL
                     LDLVCQLNLVDKVFFLGQRSDIKELMCAADLFVLSSEWEGFGLVVAEAMACERPVVAT
                     DSGGVKEVVGPHNDVIPVSNHILLAEKIAETLKIDDNARKI"
BASE COUNT          131 a           56 c           97 g          148 t
ORIGIN      
        1 taggaagact tgttgaagca aaagactatc cgaacttatt aaatgcaata aatcatttga
       61 ttctttcaaa aacatcaaat tgtaatgatt ttattttgct tattgctggc gatggcgcat
      121 taagaaataa attattggat ttggtttgtc aattgaatct tgtggataaa gttttcttct
      181 tggggcaaag aagtgatatt aaagaattaa tgtgtgctgc agatcttttt gttttgagtt
      241 ctgagtggga aggttttggt ctcgttgttg cagaagctat ggcgtgtgaa cgtcccgttg
      301 ttgctaccga ttctggtgga gttaaagaag tcgttggacc tcataatgat gttatccctg
      361 tcagtaatca tattctgttg gcagagaaaa tcgctgagac acttaaaata gatgataacg
      421 caagaaaaat aa
//
LOCUS       DAZUFB010000485          431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZUFB010000485 DAZUFB010000000
VERSION     DAZUFB010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.496"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..424
                     /locus_tag="ACHY3M_005660"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHY3M_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          117 a           83 c          136 g           95 t
ORIGIN      
        1 atttttggta aaaaaagccc gcagagcggc aacggaaatt aaaaagtttg aaaaacgcga
       61 tctggcacag gtggtgatta acgccgccta cctggtggcc tatgcagatg gtgaatgtga
      121 ggcttcctag aaagcgaaga tcgaacaggt actgcgtaat cagcctgcgc tgtacgcgtt
      181 tacgtcagaa attaatgcga ttagcgcaac cattatcggt cagctggata cgaactttaa
      241 aactggtcgt cgtgcggcgt tacgtgagat cgaggatgtg aaacacgata cgcgtgaagc
      301 ggaagatgtg ctggatgtgg cggtggccat tgcggaggca gacggcgaaa ttgagccgga
      361 agagcgcaag gtgctggaag agattgccgg tgttctgggt cttcgtctgg agaattacct
      421 gtgacggtaa a
//
LOCUS       DAZUFB010000486          431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZUFB010000486 DAZUFB010000000
VERSION     DAZUFB010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.497"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>431)
                     /locus_tag="ACHY3M_005661"
     CDS             complement(<1..>431)
                     /locus_tag="ACHY3M_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFS4320303.1"
                     /translation="LCRMAAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNP
                     NTRTEVSTLCISQFRYSAQIRSSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVY
                     DYPGRFKGAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT           78 a          124 c          127 g          102 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcgactc attccccgcg cggtttctgc attgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cccccttgaa acgtccgggg
      121 tagtcataca cttcatactg tgtgcgctgg taatcctggt gctggccttc ctgatcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagagcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca g
//
LOCUS       DAZUFB010000487          428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZUFB010000487 DAZUFB010000000
VERSION     DAZUFB010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.498"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>428)
                     /locus_tag="ACHY3M_005662"
     CDS             complement(<1..>428)
                     /locus_tag="ACHY3M_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4320304.1"
                     /translation="GRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWL
                     GWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKT"
BASE COUNT           87 a          171 c           89 g           81 t
ORIGIN      
        1 gttttgatgg ccccgcccag cttccagccc tggcttatcc ccaccacact ctcatcgggg
       61 atcccggctt ccgccagtga atccagaatc tgccccacac ctgacgggtc aataccgata
      121 tgatccagta actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca
      181 tccccgaccc gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat
      241 gcctcactct tccgtctgac caccgcggtt tcatgcaccc aggcatggcc ccagcccagc
      301 cattcgcggg tttccctgtc acggccagtc acgtacattc ccagcagatc atccaggccc
      361 ccgccgtcaa tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg
      421 cgcctgcc
//
LOCUS       DAZUFB010000488          405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZUFB010000488 DAZUFB010000000
VERSION     DAZUFB010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.499"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>405
                     /locus_tag="ACHY3M_005663"
     rRNA            <1..>405
                     /locus_tag="ACHY3M_005663"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          106 a           97 c          124 g           78 t
ORIGIN      
        1 gcggtccggc cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg
       61 acgtcaagtc atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca
      121 aagagaagcg acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg
      181 agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg
      241 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa
      301 agaagtaggt agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt
      361 gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg atcac
//
LOCUS       DAZUFB010000489          389 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZUFB010000489 DAZUFB010000000
VERSION     DAZUFB010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.500"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>389)
                     /locus_tag="ACHY3M_005664"
     CDS             complement(<1..>389)
                     /locus_tag="ACHY3M_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS4320305.1"
                     /translation="VDNGASFEPLSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDT
                     PRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAVPATIELTPGYFQIT"
BASE COUNT           70 a          129 c          110 g           80 t
ORIGIN      
        1 gttatctgaa aatagcccgg cgtcagctca atcgtggcgg gtaccgcagg tgcattaatc
       61 ctgaacgtgg tggtggccgg ttccccctgc tggccataac tgttaatcgc cctgactgtc
      121 agggtgtatt ccccgagcgg cagaccactg aaacgatgct ctgtatccgc agtgatggcg
      181 gtggtcacca gacggctgtc ttctccgctt ccgctggtca ggcgcagact gaagcgcaca
      241 cccttcacca cccgcggcgt gtcccatttc gcctgcgcca gatactgacc gtcagccgcg
      301 ctcacctcca ccgtcaggtg ctgcacagcc ggcgggatga cgctgttcag cgaaccggac
      361 agtggctcaa agctggcccc gttatccac
//
LOCUS       DAZUFB010000490          388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZUFB010000490 DAZUFB010000000
VERSION     DAZUFB010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.501"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>388)
                     /locus_tag="ACHY3M_005665"
     CDS             complement(<1..>388)
                     /locus_tag="ACHY3M_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4320306.1"
                     /translation="LTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFG
                     GGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNL
                     YRLMRGYAEGGYVGGAGSPAQMRRAEG"
BASE COUNT           84 a          149 c           87 g           68 t
ORIGIN      
        1 ccttccgccc gccgcatctg cgccggactt ccggcaccac ccacataacc accttccgca
       61 tagccgcgca tcagacggta aagattcccc acacctatcc ggctggttgc ctctttcgtg
      121 aaaacaaact ccccgcggtg aactatcccc gcaggctcat atttgccgcc cgtccccgta
      181 aatcctccgg tcgcgaaatg gaagttcgcc gccgcagcct caatagccgt ccccgaggaa
      241 gcagatgcac caccaccgaa agcaccgcca atggcgctgc cgatacgccc gacaatgccc
      301 accatggcct gtttaagcag gatttctgtc atcatggaca gcaccgaacg ggtgaatccc
      361 cgccagtctg cctctgcacc ggtcagca
//
LOCUS       DAZUFB010000491          383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZUFB010000491 DAZUFB010000000
VERSION     DAZUFB010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.502"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..220
                     /locus_tag="ACHY3M_005666"
     CDS             <1..220
                     /locus_tag="ACHY3M_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4320307.1"
                     /translation="PVMKAGAERSWLSGGRGKKNKKIPQSCGTEGALRRLSVRNYSYV
                     TTKNINAEKLLAPQSVCKNVKIFRSFIQ"
BASE COUNT          120 a           61 c          114 g           88 t
ORIGIN      
        1 acccgtgatg aaggcggggg cggaaaggtc gtggttatcg ggcggcaggg gtaaaaagaa
       61 taaaaaaatc ccgcagagtt gcggaactga gggagcgtta cgaagattaa gtgtaaggaa
      121 ttattcttat gtcacgacaa aaaacattaa cgcagagaaa ttattagcac cacagtcagt
      181 ttgtaaaaat gtgaagatat tcagaagttt tattcagtga tgatacaggc atcctccggg
      241 atgcctgttg tttttgtgcg taacagttat cacagtaaag ggtgagacaa tgggcaaagg
      301 tggtggcaag gcgcacacgc cggttgaggc aaaggacaat cttaagtcca cgcagatgat
      361 gagcgtgatt gatgcgattg gtg
//
LOCUS       DAZUFB010000492          380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZUFB010000492 DAZUFB010000000
VERSION     DAZUFB010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.503"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          120 a           63 c           79 g          118 t
ORIGIN      
        1 gtgaaggcct ccgcaagtgt tcgtctggct tcacatggca tcttcttctt agaaaaatat
       61 cgacatattt tgtgacacga attgcaaatc tggttttgtt gtatggattg cgtgattttt
      121 gatctggtat aacaggtata aaggtgcacc aagatagtca atgagacagg gcatctcgca
      181 atctatggca aacatcactt cagttctttc tcatcgggtg atgaaaacgc acttcagtct
      241 gaaaggaata tgaaaatgag atcaacagac attctatttt atgactctgg gtaaaatgga
      301 ttgagtaagt gatatagctt acgaacattc aaatcaatta aacatcagaa gagattttat
      361 actcaggtat ttaatctgga
//
LOCUS       DAZUFB010000493          375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZUFB010000493 DAZUFB010000000
VERSION     DAZUFB010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.504"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..367
                     /locus_tag="ACHY3M_005667"
     CDS             2..367
                     /locus_tag="ACHY3M_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4320308.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCHCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT           76 a           95 c          119 g           85 t
ORIGIN      
        1 catggctgtg cttcgtacgt taccgggcag aatcaaaact ctgaacaccc ggcgggtgaa
       61 tattctgaag ggtgaacagc gtcgtgtcag tggcagtgca cgtgtttccc tcaagcgtca
      121 tatctggctc agggatgccg ggcagtgctg tcactgtggt cgtgtggttg acctctgtga
      181 cagtgaactc gatcaccgaa ttgcacttca gttcggtggt ggtaatgagg agacgaatct
      241 ctggaccctc tgtactgaat gccatcgaca aaagtctgct cgtgaagcgg cgggtggtat
      301 gccggacccg acgctgccgg aggtgtccgg aggtcatggc agggcagacg atatcatcgg
      361 actgtgaccc gcccc
//
LOCUS       DAZUFB010000494          375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZUFB010000494 DAZUFB010000000
VERSION     DAZUFB010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.505"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>375
                     /locus_tag="ACHY3M_005668"
     CDS             <1..>375
                     /locus_tag="ACHY3M_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFS4320309.1"
                     /translation="LDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQ
                     YFKLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQ
                     AIETLSAIGLSYEKYKTANNVYF"
BASE COUNT          134 a           61 c           72 g          108 t
ORIGIN      
        1 ttggatgaag cagtctgggt caaacaagca ctggaaaaaa atcaaccagg aaagccagta
       61 cgggtgataa atcctgatga tgaatcgata cgaatttttt catggcttgc tgataatttc
      121 cctgatttac aatattttaa acttcaacta ttagatgcca gtaatctaag attgacagtg
      181 agtaagcaac ggaacgctat cacacagcaa ctaatcgata atcttattaa aggattactg
      241 caaactatgc catatgccag caatataagt attgcggtat tagatgataa tgtattggaa
      301 agtcaggcta ttgaaacgtt gtcagcgata ggtctttctt atgaaaaata taaaacagct
      361 aacaatgtgt atttc
//
LOCUS       DAZUFB010000495          375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZUFB010000495 DAZUFB010000000
VERSION     DAZUFB010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.506"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..374)
                     /locus_tag="ACHY3M_005669"
     CDS             complement(<1..374)
                     /locus_tag="ACHY3M_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4320310.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGT"
BASE COUNT           89 a          103 c           95 g           88 t
ORIGIN      
        1 ttggtaccgg atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg
       61 gacggaaact gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg
      121 ttctgggcct gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga
      181 ccgttgatgt tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctga
      241 ccggcggtga actggacttc cttgatggtg cagtccaggc gcagatattc cgccttatcc
      301 atagtttcag cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg tgagcgttca
      361 tacaaagcag acatt
//
LOCUS       DAZUFB010000496          371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZUFB010000496 DAZUFB010000000
VERSION     DAZUFB010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.507"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..314)
                     /locus_tag="ACHY3M_005670"
     CDS             complement(<1..314)
                     /locus_tag="ACHY3M_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFS4320311.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWILLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYG"
BASE COUNT          109 a           57 c           64 g          141 t
ORIGIN      
        1 ccatatgggc atatttttat aatgcaacta ttatgcaaac atttatttgt tattttgctt
       61 tctcctggag gacactcttg actgcttttg agtagaatcc ataaatcctt gttgaatggt
      121 gcgatgttat aaatagtaat aggatattct ttatctttaa ggataattcc agatttaacc
      181 ggtgtaaata tactgccagg agggagaaat atagtagatt gataccagat gatcattttc
      241 atattacccc atatggctga aaaagatatg ccgcatgaag gttgaattat cgtgtcaatt
      301 actatccact tcatttgtta tgtcttatcc cacggtattt aatatagttc atttggatgt
      361 tcatttcttt a
//
LOCUS       DAZUFB010000497          361 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZUFB010000497 DAZUFB010000000
VERSION     DAZUFB010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.508"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..>361
                     /locus_tag="ACHY3M_005671"
     CDS             21..>361
                     /locus_tag="ACHY3M_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFS4320312.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVL"
BASE COUNT           98 a           79 c           95 g           89 t
ORIGIN      
        1 cataattttc agggggagtt atggatcgtg aattaaaaaa tctgcatctg aatatttccc
       61 aactggccgc attatccggt gctcatcgac agactgttgc ggctcgggta aaaaacataa
      121 gcccagccgg tggtcatgag agcaatctca aactgtaccg actgacagat atccttgccg
      181 agctgatgaa agctcctctg cctgtagata acgaggaaat ggatcctcat gcgcgtaaag
      241 catggtacca gtcagaacgt gaccgactga aatttgagca ggaaactggt cagcttgtgc
      301 cagtcagtga tgtcaggcgg tccttttctg tcgtggtgaa agcgatagtt caggtactgg
      361 a
//
LOCUS       DAZUFB010000498          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZUFB010000498 DAZUFB010000000
VERSION     DAZUFB010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.509"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..341
                     /locus_tag="ACHY3M_005672"
                     /pseudo
     CDS             222..341
                     /locus_tag="ACHY3M_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           84 a          105 c           67 g          103 t
ORIGIN      
        1 tctcacatag gatcagatct cgtctgatcg ttccccctgc gctcactacc atggctcttt
       61 accacagcag ccggggttgt ttgggaccga cttccgacag cagatcccgg tgggcctgcc
      121 accatctctt cacaagctta tgcttattac atgcaaaacg tcccgttttg catctttgtg
      181 cctacggcac accgatgatc tttccctgtg atctttccct gaatctgccc ctgagaacct
      241 gaatcaaacg tctgactcgc agaaccatct gtttctcgcg ctctgtagaa cttcatgaaa
      301 aagtcatcgg tgcttttatc gaaaaataca tgttctacta attgaagtca tcatcaaaa
//
LOCUS       DAZUFB010000499          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZUFB010000499 DAZUFB010000000
VERSION     DAZUFB010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.510"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHY3M_005673"
     CDS             <1..>356
                     /locus_tag="ACHY3M_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS4320313.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZUFB010000500          351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZUFB010000500 DAZUFB010000000
VERSION     DAZUFB010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.511"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..>351)
                     /locus_tag="ACHY3M_005674"
     CDS             complement(44..>351)
                     /locus_tag="ACHY3M_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320314.1"
                     /translation="TRFKDKYLEVESYFVDKSIWNVHITFHADKRFVFFTDQFIAEFS
                     PQEFVDNCEQYLINNHCFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
BASE COUNT          126 a           61 c           53 g          111 t
ORIGIN      
        1 tacaattaga ttatattcaa atcattaaac ttgagccagg gagctataag tcctcagggc
       61 ttgccaaaac agaattattc atatccaaag aatgtgtttc aaacattttt tcaactaaat
      121 aatgtctggc ttgttcgcag atttcgttaa ccttgtcagg ggagaaacaa tgattattaa
      181 ttaaatattg ctcacaatta tccacaaatt cctgcggact aaattcagca ataaattgat
      241 cggtaaaaaa cacaaaacgc ttatcagcat gaaatgtaat atggacattc catattgatt
      301 tgtcgacaaa atatgactct acttcaagat acttatcttt gaatctggta g
//
LOCUS       DAZUFB010000501          347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZUFB010000501 DAZUFB010000000
VERSION     DAZUFB010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.512"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..182)
                     /gene="hokA"
                     /locus_tag="ACHY3M_005675"
     CDS             complement(30..182)
                     /gene="hokA"
                     /locus_tag="ACHY3M_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFS4320315.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT           97 a           82 c           68 g          100 t
ORIGIN      
        1 aggcgggaat aacttcccgc cgaagactct tactctttta aattgcaggc taaaaatgcc
       61 gccagctcat aactcccctg tttaatatgc aattcacaca gtgaatctct tatcatccag
      121 gtgaaaaata aaagcgtgaa acaaatcact attaaagaaa gtaatctata tttctgcggc
      181 attcccagcc ccctgtgttg atttccaacg agtaagaggc taaccttatg gtgttcaggc
      241 atagggtagc ctcactttga tttatagtca ggtggggctt ttctctgtct gcctttcggt
      301 gaatacctga gacaaacagt ctcaagcacc cgtggctatt ctagctt
//
LOCUS       DAZUFB010000502          344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZUFB010000502 DAZUFB010000000
VERSION     DAZUFB010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.513"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>344
                     /locus_tag="ACHY3M_005676"
     rRNA            <1..>344
                     /locus_tag="ACHY3M_005676"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           85 a           75 c          119 g           65 t
ORIGIN      
        1 ctgatactgg caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa
       61 tgcgtagaga tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc
      121 tcaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa
      181 cgatgtcgac ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc
      241 gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac
      301 aagcggtgga gcatgtggtt taattcgatg caacgcgaag aacc
//
LOCUS       DAZUFB010000503          344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZUFB010000503 DAZUFB010000000
VERSION     DAZUFB010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.514"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..309
                     /locus_tag="ACHY3M_005677"
     CDS             <1..309
                     /locus_tag="ACHY3M_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4320316.1"
                     /translation="GPLPENVTWLSPCGEYQKWNGTSWVKDAEAEKLFRIREAEETKN
                     SLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ
                     "
BASE COUNT          106 a           63 c          103 g           72 t
ORIGIN      
        1 ggtccattac cggaaaatgt tacctggttg tcgccgtgtg gagagtatca gaagtggaac
       61 ggcacatcct gggtgaaaga tgcagaagca gaaaaactgt ttcggataag ggaggcggaa
      121 gaaacaaaaa acagcctgat gcaggtagcc agcgagcata ttgcgccact tcaggatgcc
      181 gtagatttgg atattgcaac ggaggaagag gcatcgttac tggctgcatg gaagacatac
      241 cgggtattgt tgaatcgtgt taatacagcg gtagcagcgg atgttgagtg gccagtcgcc
      301 ccacaataaa gagaaaaagc catcgattga aatatagatg gctt
//
LOCUS       DAZUFB010000504          342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZUFB010000504 DAZUFB010000000
VERSION     DAZUFB010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.515"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHY3M_005678"
     CDS             complement(<1..242)
                     /locus_tag="ACHY3M_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS4320317.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          104 a           69 c           74 g           95 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta ag
//
LOCUS       DAZUFB010000505          336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZUFB010000505 DAZUFB010000000
VERSION     DAZUFB010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.516"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>336)
                     /locus_tag="ACHY3M_005679"
     CDS             complement(<1..>336)
                     /locus_tag="ACHY3M_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFS4320318.1"
                     /translation="CILFICSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNIS
                     VALWFLFTGILPCGLLLLVNIRYPETWIKGIIYRLISMFASLLIIFAIAFFFYKDYAS
                     VGRNNSSLN"
BASE COUNT          134 a           59 c           46 g           97 t
ORIGIN      
        1 tatttaatga agagttgttt ctgccaactg atgcataatc cttataaaag aaaaacgcaa
       61 tcgcaaatat aatcaagaga gatgcaaaca ttgatattaa acgatatata atacccttta
      121 tccatgtctc aggataacgg atatttacta acaacaatag tccacatgga agtatacccg
      181 taaataaaaa ccacagtgca acagatatat tgaaatatga tgtcatttca cctgcatttg
      241 tttcaaaaat attctgcatc attgttttat caaactgcac accatatttc atggtggcat
      301 atgtaacaat ggaactacag ataaacagaa tacaaa
//
LOCUS       DAZUFB010000506          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZUFB010000506 DAZUFB010000000
VERSION     DAZUFB010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.517"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..234
                     /locus_tag="ACHY3M_005680"
     CDS             <1..234
                     /locus_tag="ACHY3M_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320319.1"
                     /translation="PIAWECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDK
                     LPREYRNGWPLAYSDYAEGWNDCREAMLQGDKS"
     gene            231..>335
                     /locus_tag="ACHY3M_005681"
     CDS             231..>335
                     /locus_tag="ACHY3M_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320320.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIM"
BASE COUNT           98 a           76 c           85 g           76 t
ORIGIN      
        1 ccgatagcgt gggaatgcgg tgaaaacata atcctgttta accctgacac agttgaagca
       61 tacgcaaaac gtgcggaaat atcacctaaa ccactattct ccgcgccgcc agcgctggta
      121 gtgcctgata agttgccgcg tgaatacaga aacggttggc ctcttgcgta tagtgattat
      181 gctgaaggct ggaacgactg ccgcgaagcc atgcttcagg gagataaatc atgattaatc
      241 gtatcaagct ggagcacatc ctcgaatatg ccaggcagca gaggcatatt ggtcagcatt
      301 gtaaaattcc accaggagat atggttgaaa tcatg
//
LOCUS       DAZUFB010000507          314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZUFB010000507 DAZUFB010000000
VERSION     DAZUFB010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.518"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHY3M_005682"
     CDS             <1..>314
                     /locus_tag="ACHY3M_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4320321.1"
                     /translation="CIIHAAGLVGGIHANISRPFDFLEKNLQMGLNLVSVAKKLGIKK
                     VLNLGSSCMYPKNFEEAIPEKALLTGELEETNEGYAIAKIAVAKACEYISRENSNYFY
                     KT"
BASE COUNT          111 a           45 c           67 g           91 t
ORIGIN      
        1 actgtattat acatgcagcg ggattagttg gaggcattca tgcaaatata agcaggccgt
       61 ttgattttct ggaaaaaaat ttgcagatgg gtttaaattt agtttccgtc gcaaaaaaac
      121 taggtatcaa gaaagtgctt aacttgggta gttcatgcat gtaccccaaa aactttgaag
      181 aggctattcc tgagaaagct ctgttaactg gtgagctaga agaaactaat gagggatatg
      241 ctattgcgaa aattgctgta gcaaaagcat gcgaatatat atcaagagaa aactctaatt
      301 atttttataa aaca
//
LOCUS       DAZUFB010000508          308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZUFB010000508 DAZUFB010000000
VERSION     DAZUFB010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.519"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>308)
                     /locus_tag="ACHY3M_005683"
     CDS             complement(<1..>308)
                     /locus_tag="ACHY3M_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4320322.1"
                     /translation="LKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSL
                     NELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLT
                     "
BASE COUNT          111 a           69 c           38 g           90 t
ORIGIN      
        1 ccgttagtct ttgttttaac cattcccgta aattaatata cattacacca gagttaaaat
       61 agctaccgtt aaaatcctca ttacacaaac gctcagcgct tttactttgc atagcatcta
      121 catcaattac cactgcccca tattcatctt taaactctaa tgctattaac tcgttcaatg
      181 aacccttaca gacgatatca gcatcaagat ataataatga atccaatcgc gcagaaaaat
      241 aatcaaatga aagcaaacga taataaatag atactggcca gatatttgat tgaggtagcg
      301 ccttcaat
//
LOCUS       DAZUFB010000509          303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZUFB010000509 DAZUFB010000000
VERSION     DAZUFB010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.520"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..>303
                     /locus_tag="ACHY3M_005684"
     CDS             38..>303
                     /locus_tag="ACHY3M_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFS4320323.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITG"
BASE COUNT           84 a           59 c           90 g           70 t
ORIGIN      
        1 tcgtgagtaa aaagattcgg tatcagaaga ggtgagtatg gctaacgcct ggctcagatt
       61 atggcatgac atgccaaatg accctaagtg gcgaacaatt gccagggtgt cagggcagcc
      121 aattgcaaca gtgatggcag tgtatatcca cctcctggtg agcgcgtcac gaaatgtcac
      181 gcgaggtcac attgatgtca cgacagaaga tttggcaagt gcgctcgacg tgacagaaga
      241 ggtaattgat tcaattttgc agacgatgca ggggcgggta cttgatggtg atttaatcac
      301 tgg
//
LOCUS       DAZUFB010000510          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZUFB010000510 DAZUFB010000000
VERSION     DAZUFB010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.521"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>302)
                     /locus_tag="ACHY3M_005685"
     CDS             complement(<1..>302)
                     /locus_tag="ACHY3M_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFS4320324.1"
                     /translation="SDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSL
                     KTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVA"
BASE COUNT           58 a           76 c           72 g           96 t
ORIGIN      
        1 gccacggttt tacagtaaaa atggtatctg ttctctgact cttccggcgt cagcccaccg
       61 ttataatggt gaggtctgac gctgttgtag tagttcaata tgtaatcatt aatttgctgc
      121 cgggcctcgt ccttgcctgc gtaaccattc gttggcaccc attctgtttt cagactgcgg
      181 aagaagcgtt ccatggggct gttatcccag cagtttcccc gccgactgac gctttgattt
      241 attctgcaac gccagagaag ttgttgatat ttaaggcctg tatactggct tccctggtcg
      301 ct
//
LOCUS       DAZUFB010000511          300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZUFB010000511 DAZUFB010000000
VERSION     DAZUFB010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.522"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..213)
                     /locus_tag="ACHY3M_005686"
     CDS             complement(25..213)
                     /locus_tag="ACHY3M_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320325.1"
                     /translation="MVNLPPELGSPDVWAGGGIGLIAGLLLRYFIVRVFIVFRLFGRE
                     WSFDLKDRTRTDNHTDPR"
BASE COUNT           85 a           72 c           68 g           75 t
ORIGIN      
        1 cgtacagggg gttttgaagg gggtttaacg ggggtctgtg tggttgtctg ttctggtcct
       61 gtccttgagg tcaaacgacc attcacgacc aaacaatctg aacacgataa acactctgac
      121 gatgaaatat cgaagtagca gacctgcaat aagtccaata cctccaccag cccatacatc
      181 aggtgacccc agctcaggag ggagatttac cattagtttt ccttgtaaga cgctctcaat
      241 cgcattctga cacaaaaaag tacctgacct tatgagttga cctgaagagg ccatttaact
//
LOCUS       DAZUFB010000512          295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZUFB010000512 DAZUFB010000000
VERSION     DAZUFB010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.523"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           98 a           62 c           47 g           88 t
ORIGIN      
        1 tattttctga aactctcaga ataatttcaa gattatatat tagccatttt gatgagtaat
       61 ttagattatt ggtgaacaga gtggtgctct ctgctcgcta attccgcaag cctgacaaaa
      121 ccgaagtgaa taaaaatgca atataacaga ataaaagaaa acacccctca ctggtgcttt
      181 ctgctgttaa tgtacatttt ctggcataaa aaatgctgta aatgacccgg tcacgacata
      241 caccgaattc tctacgctta tagcgttacc tcacccccaa actttcatta gtgaa
//
LOCUS       DAZUFB010000513          288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZUFB010000513 DAZUFB010000000
VERSION     DAZUFB010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.524"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..267
                     /locus_tag="ACHY3M_005687"
     CDS             40..267
                     /locus_tag="ACHY3M_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4320326.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT           97 a           66 c           55 g           70 t
ORIGIN      
        1 aaaatcctcg ccatatagct tgtcaccaga ggaaagaaaa tgtcatcgaa aaaccggccc
       61 cgcagaacaa caacccgcaa catccgattt ccaaaccaga tgattgaaca aattaacatc
      121 gctcttgacc agaaaggttc agaaaatttt tctgcgtggg tcattgaatc ttgccgccgg
      181 gagctggcag cagacataaa atatgcccgt cagttgacta taaaaaagaa tgatacacag
      241 tatgctctgc gatggctgtt catataacta tttctttata ttgctgaa
//
LOCUS       DAZUFB010000514          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZUFB010000514 DAZUFB010000000
VERSION     DAZUFB010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.525"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>287
                     /locus_tag="ACHY3M_005688"
     CDS             <1..>287
                     /locus_tag="ACHY3M_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320327.1"
                     /translation="IQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYG
                     MGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDV"
BASE COUNT           67 a           76 c           95 g           49 t
ORIGIN      
        1 attcaggtgc cgtcaaacta tgacccggaa aaacgcacgt acagcggcat ctgggacggc
       61 agcctgaaac cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac
      121 ccgcgctacg ggatgggaaa acgtctgggg gcggcggatg tggacaaatg ggcgctgtat
      181 gccattgcgc agtactgcga ccagacggtg ccggatggtt tcgggggcac agagccgcgg
      241 atgaccttta atgcatacct gtcacaacag cgtaaggcgt gggacgt
//
LOCUS       DAZUFB010000515          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZUFB010000515 DAZUFB010000000
VERSION     DAZUFB010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.denovo.526"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          106 a           53 c           43 g           85 t
ORIGIN      
        1 taatacttgc aaatattcca ccgccatcaa atgctcttgt gtgtggattt accccaatcg
       61 atccaaataa ataataattg ccaaatataa ttagtgatga caggatcaat gaacatatta
      121 ccgctctata tttttggctt aatgacattg atacaagaaa gcaaaaataa aagaacaact
      181 cataactaag tgtccacgca actaagagta tactataccc gtaatatgga cctgcggcgt
      241 gcgagtttaa tggtataagt attaatgatt ttactacatc cattaat
//
LOCUS       DAZUFB010000516         4778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.208, whole genome shotgun sequence.
ACCESSION   DAZUFB010000516 DAZUFB010000000
VERSION     DAZUFB010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.208"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..556
                     /gene="prfH"
                     /locus_tag="ACHY3M_005689"
     CDS             <1..556
                     /gene="prfH"
                     /locus_tag="ACHY3M_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFS4320328.1"
                     /translation="KALDRLIKEAARQDVAVTVLETETGHYSDTLRSALISLDGDNAW
                     ALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQ
                     HVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSLR
                     RMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(613..2070)
                     /gene="pepD"
                     /locus_tag="ACHY3M_005690"
     CDS             complement(613..2070)
                     /gene="pepD"
                     /locus_tag="ACHY3M_005690"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS4320329.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2331..2789
                     /gene="gpt"
                     /locus_tag="ACHY3M_005691"
     CDS             2331..2789
                     /gene="gpt"
                     /locus_tag="ACHY3M_005691"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4320330.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2881..4125
                     /gene="frsA"
                     /locus_tag="ACHY3M_005692"
     CDS             2881..4125
                     /gene="frsA"
                     /locus_tag="ACHY3M_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS4320331.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4183..4584
                     /gene="crl"
                     /locus_tag="ACHY3M_005693"
     CDS             4183..4584
                     /gene="crl"
                     /locus_tag="ACHY3M_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS4320332.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4623..>4778)
                     /locus_tag="ACHY3M_005694"
     CDS             complement(4623..>4778)
                     /locus_tag="ACHY3M_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS4320333.1"
                     /translation="DLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVA
                     VGMTYQF"
BASE COUNT         1165 a         1171 c         1253 g         1189 t
ORIGIN      
        1 aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct
       61 ggaaacagaa acgggccact actctgacac gctgcgttcg gcgctgattt ctctggatgg
      121 cgataacgca tgggcattaa gcgaaagctg gtgtggcact attcagtgga tttgtccgag
      181 tccgtatcgg cctcatcatg ggcgcaaaaa ctggtttctg ggcattgggc gttttaccgc
      241 tgatgagcag gaacaatcgg atgcaatccg ttatgagacg ttgcgttcgt cggggccggg
      301 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
      361 tagcgtgaag gttcagtcag agcgtagtca gcatgctaac aagcggctgg cacgattgct
      421 gattgcctgg aagctggagc agcagcaaca ggaaaatagc gcggtgctga aatcgctgcg
      481 gcgaatgttc catcaccaga ttgaacgtgg caacccgaga cggacattta cagggatggc
      541 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
      601 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gcgtccagta
      661 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
      721 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
      781 cgcgtggata atctggatgt tcggtgtttt gttgaacaga cgctgatagg tttcacgtac
      841 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
      901 ggttttcgcg ccagctagtt tacccagcga atccagcatg ctcaccacgt agtctttacc
      961 gctgtcgatc agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac
     1021 cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac
     1081 accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
     1141 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag
     1201 ctcgtttttc aggatctcct gataggtatt caccagagat ttcaggacgt cgactttatc
     1261 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc
     1321 gttgaaatcg ataaggcgca gatccagttc ttccgcatga cccgccagga agcgcaccag
     1381 tagtttgttg gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag
     1441 accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg
     1501 caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc
     1561 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
     1621 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt
     1681 ttcgtcagcc agaaccgcca gcgcagacgc cataccaatg ccgttatctg cacccagcgt
     1741 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
     1801 gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac
     1861 cggtttacga ttttccatac ctgcggtagc aggtttacga atcaggatat tacctacctg
     1921 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
     1981 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
     2041 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
     2101 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
     2161 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     2221 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     2281 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     2341 aatacatcgt cacttgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     2401 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg
     2461 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     2521 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     2581 tcatcgttat tgatgacctg gtggataccg gtggtactgc tgttgcgatt cgtgaaatgt
     2641 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     2701 actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg
     2761 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     2821 ttctttttaa cttcaggcag gttacaatag tttccagtaa gtattctgga ggctgcatcc
     2881 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     2941 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggctcttgat
     3001 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggt
     3061 attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga tgctgaacgt
     3121 accgacgatg atttatacga tactgtgatt ggctaccgtg gtggcaactg gatttatgag
     3181 tgggccaccc aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc
     3241 agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg
     3301 aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta tgaagaggcc
     3361 gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc
     3421 atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt attaatgtgt
     3481 ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg
     3541 cgcggcattg cgatgctgac tattgatatg ccatcggtgg gcttttcttc aaaatggaag
     3601 ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg
     3661 tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg
     3721 cgtctggcat atcttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt
     3781 cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt
     3841 ctggcgagtc gtttggggat gcatgatgct tccgatgacg cgttacgcgt ggagctgaat
     3901 cgctattcat taaaagtgca aggattgctg gggcgtcgct gcccaacgcc aatgttatca
     3961 ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca
     4021 tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa ttttgacaaa
     4081 ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt tgctaaattt
     4141 tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt accgagtgga
     4201 cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt
     4261 aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg
     4321 gcaccggaag tgcgtgagtt ctggggctgg tggatggagc ttgaagcgca ggaatcccgt
     4381 tttacctaca gttaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg
     4441 gtaaaagaca ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag
     4501 ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcggatga ttttcgtgac
     4561 gagccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaataat
     4621 cattaaaact gataggtcat gccaaccgcg acaatatcat cattattaat attcaatttg
     4681 ttatcgctat ccagttggtt gattttataa tcaacaaacg ctgacatatt tttgttgaaa
     4741 taatatgtag ccccgacatc gatataattg accagatc
//
LOCUS       DAZUFB010000517         3899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.231, whole genome shotgun sequence.
ACCESSION   DAZUFB010000517 DAZUFB010000000
VERSION     DAZUFB010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.231"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /gene="frdB"
                     /locus_tag="ACHY3M_005695"
     CDS             <1..276
                     /gene="frdB"
                     /locus_tag="ACHY3M_005695"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS4320334.1"
                     /translation="LCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLN
                     SQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            287..682
                     /gene="frdC"
                     /locus_tag="ACHY3M_005696"
     CDS             287..682
                     /gene="frdC"
                     /locus_tag="ACHY3M_005696"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS4320335.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            693..1052
                     /gene="frdD"
                     /locus_tag="ACHY3M_005697"
     CDS             693..1052
                     /gene="frdD"
                     /locus_tag="ACHY3M_005697"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS4320336.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1115..2248
                     /gene="ampC"
                     /locus_tag="ACHY3M_005698"
     CDS             1115..2248
                     /gene="ampC"
                     /locus_tag="ACHY3M_005698"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS4320337.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            2337..2870
                     /gene="blc"
                     /locus_tag="ACHY3M_005699"
     CDS             2337..2870
                     /gene="blc"
                     /locus_tag="ACHY3M_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS4320338.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2867..3184)
                     /gene="sugE"
                     /locus_tag="ACHY3M_005700"
     CDS             complement(2867..3184)
                     /gene="sugE"
                     /locus_tag="ACHY3M_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS4320339.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3366..3512)
                     /gene="ecnB"
                     /locus_tag="ACHY3M_005701"
     CDS             complement(3366..3512)
                     /gene="ecnB"
                     /locus_tag="ACHY3M_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS4320340.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3623..3748)
                     /gene="ecnA"
                     /locus_tag="ACHY3M_005702"
     CDS             complement(3623..3748)
                     /gene="ecnA"
                     /locus_tag="ACHY3M_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS4320341.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3800..>3899)
                     /locus_tag="ACHY3M_005703"
     CDS             complement(3800..>3899)
                     /locus_tag="ACHY3M_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4320342.1"
                     /translation="STGAVVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT          982 a          996 c         1000 g          921 t
ORIGIN      
        1 ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag agttcatcgg tccggctgcc
       61 attacgctgg cgcatcgtta taacgaagat agccgcgacc acggtaagaa ggagcgtatg
      121 gcgcagttga acagccagaa cggcgtatgg agctgtactt tcgtgggcta ttgctccgaa
      181 gtctgcccga aacacgtcga tccggctgcg gccattcagc agggcaaagt agaaagttcg
      241 aaagactttc ttatcgcgac cctgaaacca cgctaaggag tgcaacatga cgactaaacg
      301 taaaccgtat gtacggccaa tgacgtccac ctggtggaaa aaattgccgt tttatcgctt
      361 ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg ttcagcattg aactgatttt
      421 cgggctgttt gccctgaaaa atggcccgga agcctgggcg ggattcgtcg actttttaca
      481 aaacccggtt atcgtgatca ttaacctgat cactctggcg gcagctctgc tgcacaccaa
      541 aacctggttt gaactggcac cgaaagcggc caatatcatt gtaaaagacg aaaaaatggg
      601 accagagcca attatcaaaa gtctctgggc ggtaactgtg gttgccacca tcgtaatcct
      661 gtttgttgcc ctgtactggt aaggagcctg agatgattaa tccaaatcca aagcgttctg
      721 acgaaccggt attctggggc ctcttcgggg ccggtggtat gtggagcgca attattgcgc
      781 cggtgatgat cctgctggtg ggtattctgc tgccactggg gctgtttccg ggtgatgcgc
      841 tgagctacga gcgcgttctg gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc
      901 tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc
      961 tgaaaattca cgtgcctgcg ggcaaatggg ttttctacgg tctggctgct atcctgacag
     1021 ttgtcacgct gattggtatc gttacaatct aacgtatcgc caatgtaaat ccggcccgcc
     1081 tatggcgggc cgttttgtat ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt
     1141 attaattacc gcctcttgct ccacatttgc tgcccctcaa caaatcaacg atattgtgca
     1201 tcgcacaatt accccgctta tagagcaaca aaagatcccc ggtatggcgg tggcggtaat
     1261 ttatcagggt aaaccttatt actttacctg gggctatgcg gacatcgcca aaaagcagcc
     1321 cgtcacacag caaacgttgt ttgagttagg ttcggtcagc aaaacattta cgggcgtgct
     1381 tggtggcgac gctattgctc gaggggaaat caagttaagc gatcccacaa caaaatactg
     1441 gcctgaactt accgctaaac agtggaatgg gatcacacta ttacatctcg caacctacac
     1501 tgctggcggc ctgccattgc aggtgccgga tgaggtgaaa tcctcaagcg acttgctgcg
     1561 cttctatcaa aactggcagc ctgcatgggc gccaggaaca caacgtctgt atgccaactc
     1621 cagtatcggt ttgttcggcg cactggctgt gaagccgtct ggtttgagtt ttgagcaggc
     1681 gatgcaaaca cgtgtcttcc agccactcaa actcaaccat acgtggatta atgtaccgcc
     1741 cgcagaagaa aagaattacg cctggggata tcgcgaaggc aaggcagttc atgtttcgcc
     1801 aggggcgtta gatgctgaaa cttatggtgt gaagtcgacc attgaagata tggcctgctg
     1861 ggtacgaagc aatatgaatc cccgtgatat caacgacaaa acacttcagc aagggataca
     1921 actggcacaa tctcgctact ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat
     1981 gctggactgg ccggtaaatc ctgacagcat cattaacggc agtggcaata aaattgcact
     2041 ggcagcacac cccgtaaaag cgattacgcc cccaactcct gcagtacgcg catcatgggt
     2101 acataaaaca ggggcaaccg gcggatttgg tagctatgtc gcgtttattc cagaaaaaga
     2161 gctgggtata gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc
     2221 ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt cggacctttt
     2281 ctgcgctttt ccttgctgtc atctacactt agaaaaaaac cagtaaggaa acatttatgc
     2341 gcctgctccc tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc tgcagttctc
     2401 ctacgccgcc gcgtggcgta acagtagtga ataatttcga cgccaaacgt tatctcggta
     2461 cctggtatga gattgcccgt tttgatcacc gttttgaacg tggactggaa aaagtcaccg
     2521 caacatacag cctgcgtgat gacggcggcc tgaatgtcat taataaaggc tataaccctg
     2581 acagaggaat gtggcagcag agtgaaggga aagcgtactt taccggcgca ccaactcgcg
     2641 ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg ttataacgtt attgcactcg
     2701 atcgggaata ccgccatgcg ctggtttgcg gcccggaccg cgactacctg tggatactct
     2761 cccgcacgcc aaccatttct gacgaagtga aacaggagat gctggcagtc gcgacccggg
     2821 aagggtttga tgtcagtaag tttatttggg tacagcagcc cggtagttag tgagtgctga
     2881 gtttcagacc aataatcccc aatacgatta acgccagact cgccaggcgc atcgggttag
     2941 cggactcacc gagcagcaca atgccggtga tggccgcgcc gactgcgcca atacccgtcc
     3001 acacggcata agccgtccct actggtaacg atttcatcgc ccaggcaagt agcgccatac
     3061 tgacaatcat cgccgtcacg gtaataacac tcggcgtcaa acgactaaag ccgtgggtat
     3121 atttcaggcc aacggcccat accacttcca gcagaccagc aataactaag ataatccagg
     3181 acatatcagg ctccggagaa ttggggccgt ccccggtgaa aagaggcgtt tgcaggtcgt
     3241 cctgcaaagc tgatggttga acggatattt tgccaggtgg caggggaatt tcaactatta
     3301 ccttagtgac tatcgcagaa aaaacaaacg gcacgacata cctgtcgtac cgttattgct
     3361 tattgctatt gctgcgcttt cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg
     3421 tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag caccagaaca
     3481 gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt aatgttattt
     3541 atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgacgg aaatctgatg
     3601 atttttcgga agagaagaaa aattagctgg cagcgcgaga gatggagttg ccgagatgtt
     3661 tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt gagcagcgtg ctggcaagca
     3721 aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc
     3781 aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag cgggtatcca
     3841 ctttgatgac ttcgccgatt tgtacgaaca gcggaacctt aaccacagcg ccagtagac
//
LOCUS       DAZUFB010000518         3887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.232, whole genome shotgun sequence.
ACCESSION   DAZUFB010000518 DAZUFB010000000
VERSION     DAZUFB010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.232"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..283
                     /locus_tag="ACHY3M_005704"
     CDS             <1..283
                     /locus_tag="ACHY3M_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320343.1"
                     /translation="MGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQR
                     RFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            270..1121
                     /gene="repC"
                     /locus_tag="ACHY3M_005705"
     CDS             270..1121
                     /gene="repC"
                     /locus_tag="ACHY3M_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFS4320344.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            1429..2244
                     /gene="sul2"
                     /locus_tag="ACHY3M_005706"
     CDS             1429..2244
                     /gene="sul2"
                     /locus_tag="ACHY3M_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFS4320345.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2305..3108
                     /locus_tag="ACHY3M_005707"
                     /note="aph(3'')-Ib"
     CDS             2305..3108
                     /locus_tag="ACHY3M_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFS4320346.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3108..3875
                     /locus_tag="ACHY3M_005708"
     CDS             3108..3875
                     /locus_tag="ACHY3M_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS4320347.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGGDAANLLI"
BASE COUNT          760 a         1096 c         1184 g          847 t
ORIGIN      
        1 gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg tcgataacat
       61 ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat ggggtgtgga
      121 cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact atggcgcacc
      181 gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg ccgtgctgga
      241 gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag cacagcctca
      301 gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt gccctcaagc
      361 ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga
      421 tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg caagggctgg
      481 tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc gaaggcggac
      541 ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgat gccatggtgg
      601 tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat agtggtgggg
      661 cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc atcgcccaga
      721 atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac gaggcggacg
      781 ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt ggcggccagc
      841 atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc cgcctgctgc
      901 accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc atagatacct
      961 tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc
     1021 gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc gagttcgcgg
     1081 cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca ctctattgta
     1141 aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt ggtaatacca
     1201 tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc tactgagcgc
     1261 tgccgcacag ctccataggc cgctttcctg gctttgcttc cagatgtatg ctattctgct
     1321 cctgcagcta atggatcacc gcaaacaggt tactcgcctg gggattccct ttcgacccga
     1381 gcatccgtat gagactcatg ctcgattatt attattatag aagcccccat gaataaatcg
     1441 ctcatcattt tcggcatcgt caacataacc tcggacagtt tctccgatgg aggccggtat
     1501 ctggcgccag acgcagccat tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg
     1561 atcgacctcg gtccggcatc cagcaacccc gacgccgcgc ctgtttcgtc cgacacagaa
     1621 atcgagcgta tcgcgccggt gctggacgcg ctcaaggcag atggcattcc cgtctcgctc
     1681 gacagttatc aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat
     1741 gatattcgcg gttttccaga cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa
     1801 ctcgtcgtta tgcattcggt gcaagacggg caggcagatc ggcgcgaggc acccgctggc
     1861 gacatcatgg atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc
     1921 ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg ggttttttct gggggctgct
     1981 cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg
     2041 ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg
     2101 ggggatgtcg gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac
     2161 ttcatccgca cacacgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgcta
     2221 aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata ttcgcgcttc
     2281 atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg tgaatcgcat
     2341 tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac
     2401 gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt
     2461 gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat caactggcag
     2521 gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc ggcggctgat
     2581 ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt
     2641 cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc
     2701 gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga cgaggacaag
     2761 agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt gcggctcgac
     2821 caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa cttcatggtg
     2881 gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg aacagcagat
     2941 cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc agcgccagat
     3001 gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc
     3061 gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg ttcatgccgc
     3121 ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac accttttcca
     3181 gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag ggattgaaac
     3241 ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg cgcaatggga
     3301 ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg
     3361 agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc gaaattgcag
     3421 cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct gcccttctcc
     3481 cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt
     3541 gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc aatgcctcgg
     3601 aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc agtcgcggct
     3661 ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt
     3721 tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt gcacagatgg
     3781 cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg
     3841 cttatggggg tgatgctgcc aacttactga tttagtgtat gatggtg
//
LOCUS       DAZUFB010000519         3887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.233, whole genome shotgun sequence.
ACCESSION   DAZUFB010000519 DAZUFB010000000
VERSION     DAZUFB010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.233"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..283
                     /locus_tag="ACHY3M_005709"
     CDS             <1..283
                     /locus_tag="ACHY3M_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320348.1"
                     /translation="MGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQR
                     RFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            270..1121
                     /gene="repC"
                     /locus_tag="ACHY3M_005710"
     CDS             270..1121
                     /gene="repC"
                     /locus_tag="ACHY3M_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFS4320349.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            1429..2244
                     /gene="sul2"
                     /locus_tag="ACHY3M_005711"
     CDS             1429..2244
                     /gene="sul2"
                     /locus_tag="ACHY3M_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFS4320350.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTPEPRPLRDGLAVLAALKETARIR"
     gene            2305..3108
                     /locus_tag="ACHY3M_005712"
                     /note="aph(3'')-Ib"
     CDS             2305..3108
                     /locus_tag="ACHY3M_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFS4320351.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3108..3875
                     /locus_tag="ACHY3M_005713"
     CDS             3108..3875
                     /locus_tag="ACHY3M_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS4320352.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGGDAANLLI"
BASE COUNT          759 a         1097 c         1184 g          847 t
ORIGIN      
        1 gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg tcgataacat
       61 ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat ggggtgtgga
      121 cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact atggcgcacc
      181 gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg ccgtgctgga
      241 gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag cacagcctca
      301 gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt gccctcaagc
      361 ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga
      421 tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg caagggctgg
      481 tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc gaaggcggac
      541 ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgat gccatggtgg
      601 tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat agtggtgggg
      661 cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc atcgcccaga
      721 atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac gaggcggacg
      781 ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt ggcggccagc
      841 atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc cgcctgctgc
      901 accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc atagatacct
      961 tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc
     1021 gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc gagttcgcgg
     1081 cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca ctctattgta
     1141 aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt ggtaatacca
     1201 tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc tactgagcgc
     1261 tgccgcacag ctccataggc cgctttcctg gctttgcttc cagatgtatg ctattctgct
     1321 cctgcagcta atggatcacc gcaaacaggt tactcgcctg gggattccct ttcgacccga
     1381 gcatccgtat gagactcatg ctcgattatt attattatag aagcccccat gaataaatcg
     1441 ctcatcattt tcggcatcgt caacataacc tcggacagtt tctccgatgg aggccggtat
     1501 ctggcgccag acgcagccat tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg
     1561 atcgacctcg gtccggcatc cagcaacccc gacgccgcgc ctgtttcgtc cgacacagaa
     1621 atcgagcgta tcgcgccggt gctggacgcg ctcaaggcag atggcattcc cgtctcgctc
     1681 gacagttatc aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat
     1741 gatattcgcg gttttccaga cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa
     1801 ctcgtcgtta tgcattcggt gcaagacggg caggcagatc ggcgcgaggc acccgctggc
     1861 gacatcatgg atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc
     1921 ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg ggttttttct gggggctgct
     1981 cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg
     2041 ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg
     2101 ggggatgtcg gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac
     2161 ttcatccgca cacccgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgcta
     2221 aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata ttcgcgcttc
     2281 atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg tgaatcgcat
     2341 tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac
     2401 gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt
     2461 gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat caactggcag
     2521 gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc ggcggctgat
     2581 ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt
     2641 cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc
     2701 gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga cgaggacaag
     2761 agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt gcggctcgac
     2821 caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa cttcatggtg
     2881 gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg aacagcagat
     2941 cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc agcgccagat
     3001 gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc
     3061 gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg ttcatgccgc
     3121 ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac accttttcca
     3181 gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag ggattgaaac
     3241 ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg cgcaatggga
     3301 ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg
     3361 agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc gaaattgcag
     3421 cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct gcccttctcc
     3481 cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt
     3541 gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc aatgcctcgg
     3601 aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc agtcgcggct
     3661 ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt
     3721 tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt gcacagatgg
     3781 cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg
     3841 cttatggggg tgatgctgcc aacttactga tttagtgtat gatggtg
//
LOCUS       DAZUFB010000520         2069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.286, whole genome shotgun sequence.
ACCESSION   DAZUFB010000520 DAZUFB010000000
VERSION     DAZUFB010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.286"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005714"
     CDS             complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFS4320353.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRR"
     gene            713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005715"
     CDS             713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFS4320354.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPGLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWGIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1944..>2069)
                     /locus_tag="ACHY3M_005716"
     CDS             complement(1944..>2069)
                     /locus_tag="ACHY3M_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS4320355.1"
                     /translation="FLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          336 a          660 c          642 g          431 t
ORIGIN      
        1 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
       61 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
      121 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
      181 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
      241 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
      301 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
      361 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
      421 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
      481 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
      541 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
      601 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
      661 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
      721 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
      781 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
      841 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
      901 gggcgcgctg tcggatcgtt tcgggcggcg gccgggcttg ctcgtctcgc tggccggcgc
      961 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
     1021 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
     1081 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     1141 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     1201 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     1261 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     1321 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     1381 acttgtcgga caggtgccgg ccgcgctttg gggcattttc ggcgaggatc gctttcactg
     1441 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     1501 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     1561 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     1621 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     1681 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     1741 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     1801 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     1861 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     1921 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     1981 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     2041 tgaagcgcac tcagcgtctg atccaagaa
//
LOCUS       DAZUFB010000521         2069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.287, whole genome shotgun sequence.
ACCESSION   DAZUFB010000521 DAZUFB010000000
VERSION     DAZUFB010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.287"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005717"
     CDS             complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFS4320356.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRR"
     gene            713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005718"
     CDS             713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFS4320357.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPGLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1944..>2069)
                     /locus_tag="ACHY3M_005719"
     CDS             complement(1944..>2069)
                     /locus_tag="ACHY3M_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS4320358.1"
                     /translation="FLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          336 a          660 c          641 g          432 t
ORIGIN      
        1 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
       61 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
      121 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
      181 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
      241 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
      301 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
      361 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
      421 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
      481 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
      541 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
      601 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
      661 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
      721 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
      781 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
      841 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
      901 gggcgcgctg tcggatcgtt tcgggcggcg gccgggcttg ctcgtctcgc tggccggcgc
      961 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
     1021 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
     1081 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     1141 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     1201 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     1261 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     1321 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     1381 acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg
     1441 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     1501 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     1561 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     1621 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     1681 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     1741 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     1801 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     1861 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     1921 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     1981 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     2041 tgaagcgcac tcagcgtctg atccaagaa
//
LOCUS       DAZUFB010000522         2069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.288, whole genome shotgun sequence.
ACCESSION   DAZUFB010000522 DAZUFB010000000
VERSION     DAZUFB010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.288"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005720"
     CDS             complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFS4320359.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRR"
     gene            713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005721"
     CDS             713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFS4320360.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWGIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1944..>2069)
                     /locus_tag="ACHY3M_005722"
     CDS             complement(1944..>2069)
                     /locus_tag="ACHY3M_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS4320361.1"
                     /translation="FLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          336 a          660 c          641 g          432 t
ORIGIN      
        1 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
       61 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
      121 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
      181 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
      241 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
      301 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
      361 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
      421 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
      481 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
      541 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
      601 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
      661 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
      721 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
      781 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
      841 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
      901 gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc
      961 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
     1021 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
     1081 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     1141 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     1201 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     1261 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     1321 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     1381 acttgtcgga caggtgccgg ccgcgctttg gggcattttc ggcgaggatc gctttcactg
     1441 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     1501 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     1561 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     1621 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     1681 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     1741 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     1801 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     1861 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     1921 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     1981 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     2041 tgaagcgcac tcagcgtctg atccaagaa
//
LOCUS       DAZUFB010000523         2069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.289, whole genome shotgun sequence.
ACCESSION   DAZUFB010000523 DAZUFB010000000
VERSION     DAZUFB010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.289"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005723"
     CDS             complement(<1..607)
                     /gene="tetR(A)"
                     /locus_tag="ACHY3M_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFS4320362.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRR"
     gene            713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005724"
     CDS             713..1912
                     /gene="tet(A)"
                     /locus_tag="ACHY3M_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFS4320363.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1944..>2069)
                     /locus_tag="ACHY3M_005725"
     CDS             complement(1944..>2069)
                     /locus_tag="ACHY3M_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS4320364.1"
                     /translation="FLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          336 a          660 c          640 g          433 t
ORIGIN      
        1 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
       61 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
      121 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
      181 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
      241 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
      301 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
      361 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
      421 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
      481 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
      541 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
      601 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
      661 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
      721 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
      781 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
      841 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
      901 gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc
      961 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
     1021 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
     1081 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     1141 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     1201 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     1261 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     1321 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     1381 acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg
     1441 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     1501 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     1561 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     1621 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     1681 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     1741 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     1801 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     1861 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     1921 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     1981 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     2041 tgaagcgcac tcagcgtctg atccaagaa
//
LOCUS       DAZUFB010000524         1729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.307, whole genome shotgun sequence.
ACCESSION   DAZUFB010000524 DAZUFB010000000
VERSION     DAZUFB010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.307"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHY3M_005726"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHY3M_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFS4320365.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHY3M_005727"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHY3M_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFS4320366.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            complement(1546..>1729)
                     /locus_tag="ACHY3M_005728"
     CDS             complement(1546..>1729)
                     /locus_tag="ACHY3M_005728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4320367.1"
                     /translation="CAIAWCRVPPGEFGLVPPNPRILAYDHQRDHTVHQSPLRTGGFT
                     MQKFSNTSIIRPATTD"
BASE COUNT          461 a          302 c          425 g          541 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgat
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgacttag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
     1561 cggtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
     1621 cgactggtga acggtatgat ctctttgatg atcgtaagcg agaatacgcg ggtttggtgg
     1681 caccaggccg aactcaccgg gaggcacccg gcaccatgca atggcacat
//
LOCUS       DAZUFB010000525         1470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.330, whole genome shotgun sequence.
ACCESSION   DAZUFB010000525 DAZUFB010000000
VERSION     DAZUFB010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.330"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..379
                     /locus_tag="ACHY3M_005729"
     CDS             <1..379
                     /locus_tag="ACHY3M_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4320368.1"
                     /translation="MISLSPPTICNSAADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGAT
                     EIAHQLSIARSTVYKILEDERAS"
     gene            562..1422
                     /locus_tag="ACHY3M_005730"
     CDS             562..1422
                     /locus_tag="ACHY3M_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFS4320369.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          397 a          326 c          372 g          375 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gccgatatga tccaactgat
       61 aaaggaattt gacgctcagg gcgtggcagt ccggttcatt gatgacggga tcagtaccga
      121 cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg ctgaacgccg
      181 gaggatccta gaacgcacga atgagggccg acaggaagca aagctgaaag gaatcaaatt
      241 tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga agggcactgg
      301 tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata aaattcttga
      361 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctggta aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      781 ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc
      961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1081 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1441 tcatatatac tttagattga tttaaaactt
//
LOCUS       DAZUFB010000526         1119 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1702S44868
            SAMN43251272-rid23067753.guided.369, whole genome shotgun sequence.
ACCESSION   DAZUFB010000526 DAZUFB010000000
VERSION     DAZUFB010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251272
            Sequence Read Archive: SRR30292748
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349631.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:41:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 126 of 268
            Pseudo Genes (incomplete)         :: 129 of 268
            Pseudo Genes (internal stop)      :: 61 of 268
            Pseudo Genes (multiple problems)  :: 43 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251272-rid23067753.guided.369"
                     /serotype="O111:H 8"
                     /isolate="1702S44868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2017-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..942
                     /locus_tag="ACHY3M_005731"
     CDS             127..942
                     /locus_tag="ACHY3M_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFS4320370.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          298 a          231 c          258 g          332 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc tctgatgtta cattgcacaa
       61 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      121 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
      181 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      241 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      301 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      361 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      421 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      481 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      541 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      601 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      661 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
      721 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
      781 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
      841 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      901 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
      961 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcttccct accctcatgg
     1021 ccgcctccca ggaatcgacc caggtgctta tcgtggacat aatcctttac tcataaattg
     1081 gcactgttgc aaagttagcg atgaggcagc cttttgtct
//
